LOCUS       DAOPCO010000001        67367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_491_length_67367_cov_5.920742, whole genome shotgun sequence.
ACCESSION   DAOPCO010000001 DAOPCO010000000
VERSION     DAOPCO010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 67367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67367
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_491_length_67367_cov_5.920742"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLS90_00005"
     CDS             complement(<1..295)
                     /locus_tag="PLS90_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00814.1"
                     /translation="MQPEFTSRELHRVEFETLVTHAEAALRARDFARATPLLREAVRR
                     APFRQDLRDKLADSLQANLAGGGRRTPPATPARRPTAPPAPPAPETQRPPIPQP"
     gene            complement(612..1400)
                     /locus_tag="PLS90_00010"
     CDS             complement(612..1400)
                     /locus_tag="PLS90_00010"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPK00815.1"
                     /translation="MHSGNGQHPVLRACGRTDVGLKRQANEDSIKLDENLRLFAIADG
                     MGGHAAGEIASRTAIETLHEFVAHAAINRDFTWPFGIDSKFSLGENVLLTGIHLANRR
                     VCRLAGENETYSGMGTTVAAIYAPERVIHIGHVGDSRVYRLRGDQLQLLTDDHSWVNE
                     QLKRNIITEDEARNHRWRNVITRALGNRQDVEVDLKSVDMQCGDLFLICSDGLTGMVD
                     DQTLHQVLSTHPQEDLQALCGRLVELANEAGGQDNISVILVRVE"
     gene            complement(1699..2745)
                     /locus_tag="PLS90_00015"
     CDS             complement(1699..2745)
                     /locus_tag="PLS90_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00816.1"
                     /translation="MAQLLPRQRVLRILDQQPVDRFPAQLNYRRALRPHLAALLGVPA
                     DRLDDALFHHFILVSAERSFPADSLIYEDEWGVYWYQREGHQLDHTPPLAHIETLPAY
                     DWPDLEHPNRYLHARARIAANAGRRFVLARLGLGLFERCWSLRGFENTLRDLMLNQAF
                     IRDLLEHIVGVQLGAAQRLIELGIDGLYLRDNFAAAFGMVLSPDAWRLLFKPRLAQLI
                     TPFKAAGKHVFFQCEGDFSAILPDLLELGVDAIGPCDPIAGGLDQLLDRIEGRIAYFG
                     GLDIDRITRADSDESVRRLVRETSALFGRSGTGWIPAFSRPLDLEFPLERLALVLDTL
                     DDSTRIPSGVISSR"
     gene            3050..4615
                     /locus_tag="PLS90_00020"
     CDS             3050..4615
                     /locus_tag="PLS90_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK00817.1"
                     /translation="MNRENALERLQSEAAPWDLVVIGGGATGLGCALDAATRGYRVAL
                     LEQADFAKGTSSRSTKLIHGGVRYLQRGELGLVREALRERGRLLRNAPHLVRPLQFLV
                     PCYHHWELPYYRAGLLAYDLLAAGRGVGRSRWRGAAACGALAPGLRREGLRGGMTFWD
                     AQFDDARLAVALARSAARAGATVLNHAAVIGFTRRAGRLGGVAARDQLTGREFEVAAR
                     GVINAAGIFSDQVRALEEPGAEPRLAPSQGIHLVLPREFLLGATAILVPRTEDGRVVF
                     CIPWEGRVLIGTTDTAVQEIALEPRPRAGEVDYLLEHAGRYLERRPRREEVLSCFAGL
                     RPLVRTRAAETARLSREHEIRVSRGGLVTIAGGKWTTYRAMAEEAVDRAAEVAGLARR
                     RSQTGDLRLVGGCEPGGADDALARYGDERAALAELQREEAEWAEPLAAGLPCLKAEIV
                     WAARHEMACTVEDVLARRTRLLFLDARAAAEAAPVAARLLARELGHDAAWQAREVREF
                     TALARRYDPRAEW"
     gene            complement(4661..5848)
                     /locus_tag="PLS90_00025"
     CDS             complement(4661..5848)
                     /locus_tag="PLS90_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HPK00818.1"
                     /translation="MAMRERYGLMNRQGKGRVLDEFCAMMACHRKHAIRALNAVAPGP
                     SRRSGPTVRYGACERQVLKAVWLAADQPCGKRLVAALPLWLPAYGRRERLSAVTRKRL
                     LTMSAATADRLLAPERVRLKPKGLCGTRPGSLLKTQIPIRGQSWDEQRPGYLEADTVA
                     HCGPSLAGDFVWSLTFTDIATGWTANRAVWNRGAVGVLEQLAEVETVLPFTILGFDCD
                     NGSEFLNHHLWAYLRQRKEPVDFTRSRPYHKNDQAYVEQKNYTHVRQLLGYERLGWHE
                     LVEPINVLYRLCDQFRNAFLPVMKLASKSREGTKIKKRYDPPQTPLARILDSPHTAQN
                     RKRLLREEISRLDPFDLKDQIETQLRQVFSLKRRLESGNESMLRYSARPEPGQVPLGN
                     VFP"
     gene            6255..7043
                     /locus_tag="PLS90_00030"
     CDS             6255..7043
                     /locus_tag="PLS90_00030"
                     /inference="COORDINATES: protein motif:HMM:NF025724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HPK00819.1"
                     /translation="MRPRLLALFALLLLPLAGCGRVHYRYYVRLDDRPLRMDLPLDLV
                     YAHPRIEAIKDRTLEVHVLSPLDSVMASTAKMTKPDDWETYYETWRRDFENVLADKIW
                     RTAIFSDVGTPSDPVPLEKPDYQLRVAVTEWNEGNRWLRWILGFGAGRTRVQWEGKLV
                     NAANGEELLLFADAREHPGGPYMIGVSFHPPKGNALIAQDLAWASRDLANALREATGI
                     DREPLGTNSFHRKYRYDNWQSQEIPRPTEEIRPPSLRVRVGGGT"
     gene            7043..9127
                     /locus_tag="PLS90_00035"
     CDS             7043..9127
                     /locus_tag="PLS90_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00820.1"
                     /translation="MDPWAPGLAARAAWQLRPESCLGAAAVAGCLLLLGWPLLPRPAR
                     SWWEQWLLRAAAGCVALPILVLVAGQVRGLLFTLPWRWLGLAGWLAFAATGIARLRAS
                     RRTAPALPGAGAPPPSLFRLLALAGWICVGHWHLQMLWPALSPPLSYDVLEYHLALVT
                     HGFATGRFEPIPGILYTRQPVATEALFTLAAAAEGWARGAAPGLLNWLLFLMATLLFA
                     PLARRLRLPAPVRPWLWLAVLAHPLVFKLQLDNFTDWTGALLMLAGLLLATGRRGRAW
                     AFPLGLCAGGAVAAKWTNAGTVALPLLAVVVATARGKPTCGRELAGFGAGAAAALAPW
                     LIWLGRTVGNPFAPFLAGLFPTDAWSAERLQFLLATHQPFSPLDVDYWTNLVRKLAVE
                     LDAWPMIAVALVLSAGAVALRRRRGARWLADRSARTAAALLLGLVFSLLLWGQLRHAA
                     DRFLAPVIVLSALLLGDALRVVQRAAGPRRGTFVMVSSAVILAALALWPLRLQLATLR
                     ALAMPAVAMGRVSLPEYLHNGLGATAAMFAAVDRLPADARLIAIHEARGYYFRRPVAL
                     ASVFDRTPIRDALRGADSAESIRRRLADAGYTHVLVNEFEQARLLAMHPPLRLEDDPR
                     RRRLVERDDQTGLVRTFPAEAEFAVDPPAPAEKQAYLEFLAALRARPVWIEAPPAGTP
                     AIWIADLRPTLP"
     gene            9252..10406
                     /gene="dnaJ"
                     /locus_tag="PLS90_00040"
     CDS             9252..10406
                     /gene="dnaJ"
                     /locus_tag="PLS90_00040"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPK00821.1"
                     /translation="MATGSKDYYKTLGVERSASPDEIKKAYRKLALKYHPDRNPGDPE
                     AEERFKEVSEAYEVLSDPQKRRMYDAHGYEGVRGAFRHGGFTWDDFHHASEFQDIFGD
                     LFGNLFGFGSMGGGRSRRGRPRGRDLRLRLDIDLEDVLRGKTSEITLRRLESCLKCGG
                     SGAQPGTRPETCQRCGGHGQLRVTQGFFSLTTTCDVCGGSGQVVRNPCPECGGQGRVK
                     EKVTLKVEVPRGVETGTQLRMLGEGEAGPPGGERGDLYIVLNVRPHKRFVREGFDLHC
                     ELPISFPQAALGDTISVETPWGAQELKIPAGTQPGDRFRLHNLGVPQRDHDDAMRGDL
                     YVHAKLVVPTRLTDRQRELLRDYAAASGDEKLQEDRGFFDRFKQSLGLDD"
     gene            complement(10431..11480)
                     /locus_tag="PLS90_00045"
     CDS             complement(10431..11480)
                     /locus_tag="PLS90_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK00822.1"
                     /translation="MPKVSRKAVTLSTLARELGLSTATVSYVVNGQVRQQKISPATAE
                     RVLRAAAEAGYVPNRLAHGLHIRRSGVVGVIVENFRHGWADRVLEGLQEVLDQYSFTV
                     FSALHRGDNKREQREVQSLLERRADAILTFPLPGSRSLYERIVRHGPPLLFLGDAFED
                     FPQVSYVAWDASAGVRRAVEHLAGGGRKKIGYLAVRRDSIVHRERHDAFHEALREHDL
                     PVNPAWIGEGKAESQAGLQPLVQRLIRRMFEQSRDRPDALIVASDLMALHALALLRYI
                     GLRVPEDVAVIGMGDSTSGGLPGIELTTISEPCEEIGRVAGELALHLIEDKQARPMVR
                     FVPGDRLVVRRTTAG"
     gene            12076..12747
                     /locus_tag="PLS90_00050"
     CDS             12076..12747
                     /locus_tag="PLS90_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00823.1"
                     /translation="MRRKLGWMTLMACWLLLGGLPTAEAQPGEREGRFDRMDQNSDGV
                     VTAEEFGGPAERFARLDADGDGKLTREEMRSSFQRQRGEGWDPGRWRDMMNERMKETL
                     GASDEEWAVLQPKIEKINELRMPMATGMMFGGFGMRGRGGDGDGNRGPRAGFGGEPAP
                     EAAALQEALDNQNTSAESLQEKLDAFRKAAADREAKLKEAREELRELVTPRQEALLVL
                     NGTLD"
     gene            12758..14482
                     /locus_tag="PLS90_00055"
     CDS             12758..14482
                     /locus_tag="PLS90_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPK00824.1"
                     /translation="MAELSEQTSPLMTAAGPDAMLERMVRARQLSAEDAAAFRRQHAN
                     GALDEAAVLRWLAEEYGLEYSDLSHATPDRQLLSRFPARHLLKEEVLPLRRVGDVVEV
                     ATSRLFAVEGLDMLRTLTGERLRPVLAPSAAIARETKKWLGVGADTLDTLEEQASIQV
                     IDEEDDTNVDLDAAAQDASIIRFVNQVLSDAISLRATDIHLEPFEDEMRIRYRIDGIL
                     QDVPLPGSIKRFQPAIVSRVKILSHLDIAEKRLPQDGRIKVMLERQEVDVRVSLIPML
                     HGEGVVMRLLKKDMALRGLAELKMGAREAAGFARLLGLPHGIVLVTGPTGSGKSTTLY
                     AALNEINDAERKIITIEDPVEYHIRGINQIQVNEKSGLSFARGLRSILRHDPDVILIG
                     EIRDAETARIAVQASLTGHLVFSTLHTNDAASAPTRLIDMGVEAYLVAASLEGVMAQR
                     LVRVLCPACKAVDDSAPTTAYKQQIGLPEETPLYRAVGCEACRRTGFQGRRAIFELLL
                     LDERIRELILTTRSVARLREAAREAGMLTLVEDGRRLLADGVTSVDEILRVTRDETRT
                     NGAAAKEG"
     gene            14490..15719
                     /locus_tag="PLS90_00060"
     CDS             14490..15719
                     /locus_tag="PLS90_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK00825.1"
                     /translation="MPAFSYRAQRSEGGIAEGHLTAETRQEALRQLAGMGLRPVRLES
                     AGETAAERAGAPLGWTLGRRGVSPRALENFTRQLSSLLTAGVPLSRALRILAREGSSS
                     AAAERWRAIHDLVVDGNGLADAMARHPDVFPRIYVAMVRSGETGGFLDVVLNQVADFQ
                     ARERELRGKVLSALTYPLVLMLVAVAVLIFLMTFFIPRFQVMFEGFGAALPALTRAII
                     AASHFTTRWGLLIAVALALAYYGLRLWLATAEGRRRWQQAVLRVPAVGELVSRLAMTR
                     FCRMLGTLIGAGVPLIAALRVARESLGNQVLVDAVSDAIERVQRGEALAASLRDCPRL
                     FPPTAIEMIAVAEESGQLDRELIRLSNETEKELDGRLRTAVSLTEPILLLLMAAFIGA
                     IFIGMVLPILSIQDYLS"
     gene            15832..16257
                     /gene="gspG"
                     /locus_tag="PLS90_00065"
     CDS             15832..16257
                     /gene="gspG"
                     /locus_tag="PLS90_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPK00826.1"
                     /translation="MKLRGRPTGFTLIELLVVLMIIATLAAIVVPKFAGRSRQAQETA
                     AQAQIANISTALDAFEVDNGYYPKGDDGLDELLEKPKDAKNWRGPYLKQGIPLDPWQN
                     PYIYEYPGKHNEDGYDLMSMGPDGRVGGGDDITNWSTKD"
     gene            16261..16773
                     /locus_tag="PLS90_00070"
     CDS             16261..16773
                     /locus_tag="PLS90_00070"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HPK00827.1"
                     /translation="MERRAPERPDRGFSLLELVVVLALLTVVASLSAPRLAGFMQGRT
                     VREEARRMLSMLEWARGEAIARGERVVAWFDPATSTYGVRLDTPAGGAEQPLRRYALD
                     DDLTLHGESAAANESGQLEVLFWPDGAIDPLSARRIECWEKDAPSLAIALDEPGFNFR
                     VEDPRDAPAS"
     gene            16757..17158
                     /locus_tag="PLS90_00075"
     CDS             16757..17158
                     /locus_tag="PLS90_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK00828.1"
                     /translation="MRPQADRPRGFTFIEILAAMLFMALVVPVAMQGLLLANQVTQRA
                     TRMRQAGALAERLLNEAIVTESWRDGDLAGAFERPWEGYDWRITSQPWPEDTMRLVTV
                     EVGYQVQDQRFVERLSTLASEFAEEDAGLSE"
     gene            17158..18006
                     /locus_tag="PLS90_00080"
     CDS             17158..18006
                     /locus_tag="PLS90_00080"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HPK00829.1"
                     /translation="MRGIVINISRRGLTLLELLVALAIFASLLTALFSAYWGGLRLRE
                     KAWTSMADDARRDHVGLLLRSDLRHLAAPVGVLAGPLVGESQGNALQRADRLSFTSTT
                     GLVSDAAPWGELQQVEYFLEYPLDSGQAEGFDFIRRTRRDLLATTISEDDIPGPEWRL
                     LHGVRSLMIQYFDGEAWVDSWDSTTVENENPEAIRLWIEFVEAEAGAPPRPPIDLLVQ
                     VLSQPRASATASGAAAPAGGAGGGAGGGATPETPAGSGRAGGSREGGAREGGPPNGGA
                     RGERAT"
     gene            18003..19367
                     /locus_tag="PLS90_00085"
     CDS             18003..19367
                     /locus_tag="PLS90_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HPK00830.1"
                     /translation="MNATSPLRTPSAGRRGSLLIIVLWVALGLVTLALYFGGGARLAY
                     QASANALAGRQAEQAIEGAIRYALFVLDYQVEEGRRPLLEEGSYQPSAPLGEAAFWFI
                     GRGAADDLTQTEPAWGLVDEASKLNLNTATIEMLEALPNMTADLAAAIVDWRDADEEL
                     SPGGAESTDYETLDFAYSAKNGEFESIEELRLVLGVDEMVLWGEDRNANGILDPNEND
                     GEASWPPDNADGRLDAGLAEYVTVWTRQPNTDPDGEPRLNLRGDSVQQDLRQMLVEQL
                     DESKANDVMARLGGRLGQIESPLEFLIESGLTPEEFARVEPYLTVGDGDYRVGLVNVC
                     VAPAAVLACLPGMDESQAEALVAARRDLDPAALESIAWVAAVLEEEVAREVGPWITAE
                     SWQFSLDIAAVGANGRGLRREWVVVDNEDQARVIYRRDRSRMGAPFDLSAARAQLKTI
                     TTIN"
     gene            19420..20826
                     /locus_tag="PLS90_00090"
     CDS             19420..20826
                     /locus_tag="PLS90_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00831.1"
                     /translation="MAKRITALVVIELTRERLRAVEVRPGDRDATAGPRVDEEFRLNP
                     LSDEADLVGRELRSRLHAAGIRARAAIFCPPLDQLLVQTIDLDGVAPEDLESLIALEA
                     ERAFPFPSDELIAALSRFTNAAGSARGCLAVLPVAAADQLAAIARHAGLRVVGIRPAL
                     PVTLAARSGGAEAALRLTEHELQFAVAAPDGIALLRAFQSMRPTLARDAAEADALAGE
                     LRREFRLTLRRVQTELGAAPRRIHLSGAADDCRRFMAWYRREFADSPELVYDDSASST
                     EHSILAALAATGRAALAGERPGFDYWRPAEGRWQTGLRRWRARGVIWRAAAVALALGA
                     IAVAAMAWHVWSLRRLERRWAAIAPRVERLAAMQEGLRARRAWLSAEPEQLALLHTLT
                     GAFPETGSVWARTLVIRNSEQTVQCVGLARSNRDCLAVFEALRQTAGVQDLQVVQVEG
                     GDPIQFTLSYHWQPEGAR"
     gene            20823..21371
                     /locus_tag="PLS90_00095"
     CDS             20823..21371
                     /locus_tag="PLS90_00095"
                     /inference="COORDINATES: protein motif:HMM:NF022201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HPK00832.1"
                     /translation="MSRIPRTNLLLIVGAVCIAAFLGDRLILTPQLAAWRSRAQRIEQ
                     LERDLANGEYLIEREEAIEEQWAEAMDWALPADRAQAEAELLQALNRWAGDAALRLSD
                     LKPRWREDEQLGEQLEVRLNAQGDLEDISRFLYALETSPLSLRVEGVELRARDDRGRE
                     ITLDLRISGLTRSAEPRPEAAS"
     gene            21368..21985
                     /locus_tag="PLS90_00100"
     CDS             21368..21985
                     /locus_tag="PLS90_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00833.1"
                     /translation="MRRLFVLLIACLAAPPAVFLRAQSDSDERNAEGEPPGFEAYALI
                     LERNIFDPQRRPRAPERPEEAPRDAPPPPPPRVESIELLGTWITEQQALLFTEANRDE
                     YRGTLDVGSRLGEFRIAAVEPGFAVLEGDGERLTWPVGYRLERREDEPLWRLAGRASG
                     VARDRPTTATTSSSRSSASSSSGGEAAGDMIQRMRERRLREQGAR"
     gene            21988..23466
                     /locus_tag="PLS90_00105"
     CDS             21988..23466
                     /locus_tag="PLS90_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPK00834.1"
                     /translation="MNTIHLAILSLAVWAAAAAPRAQQPLPIDVEPPAPVEELRLNFK
                     GVDLDTVLDYLSRAAGFIVVKEVDVAGTVDVISQQPLSPDEALEVLNTVLHGKGYAAI
                     RSGRILTIVDRDDARARDIPVRQGNNPDAIERSDQMVTQIIPVRYAKATELVEDVQPL
                     LPEWATLTANESSNALVLTDTQTGVRRIAEIIRALDQSISEISAVRVFNLRYADAKEV
                     ATMIQTLYQQQQAGANEGSRRREVFQRFRGPWGGGGGGGGDADQEQAASAALQAGLKV
                     VAVADERTNSIIVSAPEDLIDTIESLVAAIDRSTDILTEVRVFPLRYSNAEQMVEVIE
                     GIFAEESENQNQPQQRRFRGPGGFWGNWGRGGGGGGGGGGNNTQEQSERRVAENTVLA
                     VADTRTNSVVVSAVSAVMDQVEQVIAELDRDPARNKQVFVYSVQNASPEDVAILLQEM
                     FGGGGGSSLRRSTNQNTGGANPNPTPSLGGGGGLGGNMNRNR"
     gene            23507..24622
                     /locus_tag="PLS90_00110"
     CDS             23507..24622
                     /locus_tag="PLS90_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00835.1"
                     /translation="MNARLGTWIGCALALLAAGGGAWAQEMGAPPPAFPPPDFAMPAA
                     EAPPPRDTLGTELADDVIVQYDPETDSIIVITDQATHQQIQQVIETLDRPVPQVLIKV
                     LFLEVTHSNDLDLGVEAAYIDNDENDAADSVMTDFGVDAAALGGFYRILDEDLRVTMR
                     ALASVGKLEVLSRPSVLARNNETATITIGQEVPFIRNTRITDNGQTINTIEYEDIGII
                     LQVTPHITPERLVEMEVLPEISTLTADTVPISETVNARVIAKRSAQTRVVVADGKTVV
                     IGGLMEDQETESVNKVPILGDIPLLGAFFRRTVKEKTKTELLIFLTPYVVDGQDALDR
                     LTVAEHQRTEITPHALTEPGVRAKYLEGLPPRIETAP"
     gene            24623..26215
                     /locus_tag="PLS90_00115"
     CDS             24623..26215
                     /locus_tag="PLS90_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00836.1"
                     /translation="MDRLYRNPPGWIVYSTLALCWLLIVVWLAFEHARVRRMEREALL
                     NRARDISNTIALVTRAGVPGLIFQSRLEEALEELVDTTELEAATLLNAAGEVVASAGG
                     RELAPLEQLPQRAVRWQTDSVVVVNLVDFGRRMEDDGTTLPATIVLPRREDWPTTGFR
                     ERFNDDAATRGEIGRFSRRRSRDRDADSERIGPPPRPQDEGEPGDRAGPGDRGRDRDF
                     FRRGSIRPPWMSEERYRELIATRGLHGFILELSTDALREQLRRDRWLRVTLALGALLA
                     VGGLGLGWRGFERATRLRMRLLRAQEMNEHLREMNLAAAGLAHETRNPLNIVRGLAQM
                     IEKRPDLPAETAGQLRRIAEEVDQITFRLNEFIKFSRPPEPRIGAVALLDLCRNVIEN
                     LRHDLDEKQATIDLAGEPVQVAADEALLRQVVFNLLLNAVQAIDTGGRIRVGIEPAAR
                     DAVVLTVADSGPGIPPELHESVFRPYYTTRADGAGLGLAVVRQIVLAHQWEVAIAPAD
                     LGGAAFRISGLRLVRPPSQNAA"
     gene            26212..27558
                     /locus_tag="PLS90_00120"
     CDS             26212..27558
                     /locus_tag="PLS90_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK00837.1"
                     /translation="MTQDAAPADTPPTLPPLLVVDDDPGQRDLLQSYLTSCGFRVATA
                     ADGEQALRHVAREPVAMIVSDVRMAGMDGLTMLHRLRQSRPDLPVLLVTAYADIRDAV
                     EAIRDGAVNYLEKPIDLDELLASVRGALKLGEATPREDPLATTALGEEIVAESRPMRD
                     ALREALIVAPTRSTVLLTGRSGVGKTLVATVIHRLSPRARQPLTLYNCAAAPAEEHEA
                     ALFGGAQPDHSLFREARGGSLLIEEIQALSPVAQTRLLHWLDPAAAGPDQDERPRLIA
                     TASRDLEAAVQAGAFAEALFYRLTSFEIYLPPLCQRPDDILPLAALFQRGATHRRARF
                     SPATMTCLQTYSWPGNVRELKNAMERAALLSGGDVILPAHLPPRIRAAAEPAGPGAET
                     AGSATMEDVERSIILQTLREQNFNRSETARRLGISRRSLLYKLRRYQEQGFQIDPD"
     gene            complement(27584..28291)
                     /locus_tag="PLS90_00125"
     CDS             complement(27584..28291)
                     /locus_tag="PLS90_00125"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPK00838.1"
                     /translation="MRDRLAVFSFAAVLALVFPSLPAAQSDKGSSSPSLLDRLYPDRQ
                     TAEPVIDLSGLDTADTQVPSSSAPAAETAAPPGPARLILRRPAPSAQPTEDRPSTATA
                     TLAADEPTTAQAPVGLAPAELAAMLKATPMPRRLPPPAIDLGTLAEAATTDEDRPTTA
                     TRTADAPPPAPVRVNVASQQELARNLRIDPRRARLIIQFRETYGPFRGPDDLEQVTGI
                     TDGMVRAWETAGLLRFD"
     regulatory      28661..28790
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            29165..30445
                     /locus_tag="PLS90_00130"
     CDS             29165..30445
                     /locus_tag="PLS90_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HPK00839.1"
                     /translation="MSQLLRIRSVTALILFAVCTFTARGEWSRPHAGAGNNPLQPIPA
                     QLTNVNAAPLWGAVSLNLPGGATPTILPPYVFHPVHDAATTRVTVVALNAETGGVFWE
                     SDPLDVDNTINYLSATSTAVDPETRALYFASGNTVYRLDADTGSIVWATELTAANTTP
                     GLSSYEIINSSPQLGNGMVFIETFGGFVIANKQLVALDQTDGSIEWFVNDVGPGTSTP
                     AYVESGADKRVYSVGDLVVRCYDADDGSLIWDSLSASPSWATSYSISCSPVVEAGRLF
                     VGSLDFFNDETALYCADAATGALLWQETAPTTDCPPLVIGDRVFVYGGLTKGTLASFA
                     VADGTPGFSKEIFTGYVFRDYPAAAEDAIYLCGGGDLVVLDPADGSELSRKSAGFFGP
                     VCVDSAGGVYAHNTGALEAYGITVPVELSEFTMN"
     gene            complement(30562..30786)
                     /locus_tag="PLS90_00135"
     CDS             complement(30562..30786)
                     /locus_tag="PLS90_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00840.1"
                     /translation="MERVIRILPAGASLFVNGAGSLNGAGMGAATRQHGSAHPSIATV
                     RREAMTVFRKIRNPKNEARNNDNKRNVRKA"
     gene            30773..32011
                     /locus_tag="PLS90_00140"
     CDS             30773..32011
                     /locus_tag="PLS90_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00841.1"
                     /translation="MTRSTSGQIVWGDAPRVSLVYWEGREDAVLPHRSWFRQWDPHAG
                     WGARERIEQSFGEGGVEVGGRHPAMVALDDGSFVAVWNDYRHGLHPAYITNIEIYAQR
                     RPADGSFASSATLEQRLSDTPDNGYLPRITRLLDGRLAVAWYDFHYNNNEASELALRI
                     GAADGDFTQSLAAAPIRLTDLADRAAGDEGKSFIMPSPAPATTDTLALCWVTLAELSS
                     GLPDTSSPASLYFGLYDLTAGAWSERGRLLANRVRGYFDPPKLIRDPASGDLWLLYVD
                     SASHPSEEVYLARRPAGAGTFEDPVRITDDGNRSRYPDAAIDADGRLHLVWVDDPAGS
                     GNSHVRYAVYDPELAALTPPVDLTPEHPREWERPAIALEASGHAFVVWEENINGLQGH
                     LWFTTNWTPNAAAAAWLTAE"
     gene            complement(32187..32816)
                     /gene="purN"
                     /locus_tag="PLS90_00145"
     CDS             complement(32187..32816)
                     /gene="purN"
                     /locus_tag="PLS90_00145"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPK00842.1"
                     /translation="MLNIGVLASHGGSNMQAIIDRIEAGELEACIVMVISNNSGSGAL
                     ERARRHGLPWRHLSSRTHPDRDALDAAIRDALREAGAQLVILAGYMRPIGPKTLAAFS
                     GRILNIHPALLPDFGGAGMYGIAPHIAVLQAGARESGATVHLVTAEYDTGPVLRQKRV
                     PVLPGDTPETLQQRVLAVEHTLYSDVIADIIAGRITIEGLEGTGGAEGR"
     gene            complement(32826..34379)
                     /locus_tag="PLS90_00150"
     CDS             complement(32826..34379)
                     /locus_tag="PLS90_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00843.1"
                     /translation="MTRGRALEAEPPAAPPPAAPAHAASFARDLRLAGAAVLALLLLT
                     QLYEVWAGVDFRDLDDLMRTLTARRWAVRPFFMTDGYWLPLPAAMRGAWLRLFSPWAL
                     QYPALTLFLMRLVTLGGLWGTWRLLADVMLTLGGGRLAVWIFSTFYVFNLGAFHLAAS
                     NYSEPDAGLWMAAALAVVLRALTAGGFTRRRLALAGAALAAACLTRYEPWLSGAALGC
                     AVVLPTARTSRPLDRWAGALILIPPGAAIAVWLGVSWLRLGDPFQFLRHAGDYAGSIP
                     PVHPNAAIALTFLQINAVWMPLAAVLCGAELVRGRRRPAVRCYALLLLVSAAWAVHRQ
                     SLFPLSIRFFWDVALLTAAPAALAAERLARRLPRRTARLAVGTALVLGMLNHVGAAWR
                     QWPYIPPGLRDLARGIHARTAGPILVRDHADWRQVPALQTFRVCIVTERLILDEWLTE
                     EGRVTPEQIRAHGIGYILARRRPQALADGPPLMEDEESGWALWRCGGDGGGGGEENDN
                     VNGNDNSEN"
     gene            complement(34376..35008)
                     /gene="ruvC"
                     /locus_tag="PLS90_00155"
     CDS             complement(34376..35008)
                     /gene="ruvC"
                     /locus_tag="PLS90_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPK00844.1"
                     /translation="MPHPRPEPPTLRVLGVDPGLADVGYGLIDWQGTRRRGSLVAYGV
                     IQTPPGTPLAQRLDTIYQSLAELIETHRPDAVAVEQLYFARNVKTAMVVAHGRAACIL
                     ATARRRIELFEYTPLQIKQALTGHGRAGKAQVQMMVRAVLGLETIPRPDHAADALAAA
                     LCHAHSLMFRDKVDQAARRAAAAAADSSGDGQDPRKLLLARARRNGRKRR"
     gene            complement(35090..35839)
                     /locus_tag="PLS90_00160"
     CDS             complement(35090..35839)
                     /locus_tag="PLS90_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK00845.1"
                     /translation="MSGHSKWHSIRHKKAVVDAKRGAMFTRLIKEIAVAARMGGSDID
                     GNPRLRTAVTAARDANMPKENIERAIKKGAGELEGVSYEDFTLEGYGQAGVAILVEGT
                     TDNKNRTIPEIRAIFNKGGGSLGAQGCVAWMFTKRGVILIPTQGQSEETVMEAALEAG
                     AEDLENEGEYYRILTEPNQMEDVRAAIETKGIKVESSSLRNIPSTTVKVEGEDAPKLL
                     RLLEALEEHDDVTSVSANEEIDDAVLEQYAQ"
     gene            complement(36097..>36446)
                     /locus_tag="PLS90_00165"
     CDS             complement(36097..>36446)
                     /locus_tag="PLS90_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00846.1"
                     /translation="DEPYVDPDAVETGLVVGGAGPQAPPAARGPAGSFGGTERGVVSG
                     AAAGRSRAEAAGPAGPTDAVAAGDQARRERRQADRPGRRDRERRRAGTPGGPPLDGRN
                     EASPTQPGTETRF"
     assembly_gap    36447..36546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<36547..38759)
                     /locus_tag="PLS90_00170"
     CDS             complement(<36547..38759)
                     /locus_tag="PLS90_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPK00847.1"
                     /translation="MPLQLDRTSRIVPGARRRRAGRGLARAAAGFLGWGLALLLAGVA
                     PAQPAEGEAEPEPAPAPTATPAPPEIRIGPDEAPEVLYEAGSTPGAPVNVLESGGVAL
                     DEGEAIAVTAPASPRTTLVVRDKPINDVFNEIREKTGVRIEARGQAQGQTLSLAVRDT
                     PVEQILESIAQERGWRWVKFPDGSYQIMDQQTYVQEVQAGQVIRRVFDLKYINARELA
                     TVLEPMINREIGSIAADERSNKLIVAEMPDRMTFIESIINEYDTQLFTRVFEIRNAST
                     EEVADRLAEIKSTSAIIHVDPVNHLIIVEDTFEKIKIMEQLVELLDRDQEIFIYNLTN
                     IGLDGEIADEIVSDFIEPIVSEDALLRFQVSTGRLILKDVKAVHDKVYDILLQLDRPR
                     KQVLIEGEVLSVGMSNDLRYGLEWEYSPELIRVLGVDEDDEVNLDEETFPRVSSGSSG
                     LSVLELTDHVRAQLNAALTDSRSKILLRPRLIIANHELGTFEVTRNEPVLQTFFSNNI
                     NSDFRTRSQTTIPSGLIVDITPHISNRGLVELEINFENSTPIIVEDLGDGTRGVGTAE
                     ESTETILIIPDGETRVIGGLISRDRNEGSSGIPVLSQIPYLGALFGNKTKRDTLRNLM
                     FFITPHIIEERPKNDLIVEPVNELARQAMAESMVQAVAPPEDLNEVPPEIQEYLRQIR
                     PTAIPLEDAPTTMPIETQPIAIPDFETGGEELGAALLRDEPYVDPDAVETGLVVGG"
     gene            complement(38798..39313)
                     /locus_tag="PLS90_00175"
     CDS             complement(38798..39313)
                     /locus_tag="PLS90_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00848.1"
                     /translation="MNQRILMVINLLLVAASGGFIFYLATVEYSKPVELRDISAYLNE
                     GDETDFESPASIPSTPETPYDPLIRSPMFGALFTPTPTPTPTPTPAPEPPNLLEAVFS
                     WQISSMDENQVEFIDNRPESGEVFTMRVGETRRATDKDGQELDVKLLTINLDEFKVLL
                     GYEGQENWVAY"
     gene            complement(39310..41016)
                     /locus_tag="PLS90_00180"
     CDS             complement(39310..41016)
                     /locus_tag="PLS90_00180"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK00849.1"
                     /translation="MPVKIELLDDETLGVSFPYERGTVASIKRLQNRRWNSRDSRWEV
                     HLSHLAELLPLLGLKPGDVSEEIRGRFRRDWQRVSAAVELGPVHGRIAGSGLPLRAID
                     EATSFPIAGHQYSPRFKQGRWDGRKHLFDIRRGTFPTGLWSRVRGCLEAHGVQYTLAG
                     APRAEPGAPLACPGAPTPLREYQQRALERALAGGRGIIQIATGGGKTLLAAHLIRRIG
                     RPAFFFVHTLDLLHQTRHVLERELGVEIGILGDGQANLRPVTVATIQTATRAFEGRAA
                     PRRRRAAPDDEEARPEERELQVDEATRQQIRDALERAEVAIFDECHHVPAATFYTLAM
                     RTGRAAWRFGLSATPWRDDDSDMLLEAALGERLAVVNCSELIEQGYLVAPRIHMTPVG
                     GVRAAPGARVEYQEWYRRAIVENQQRNRVIAARARQWVADGRSVLVLVGQIAHGELLR
                     QLLPEAQFIHGLLEGEARQRLLQQLERKLHPLLIATTLADEGLDVPSLDAVILAGGGK
                     SPTRAYQRIGRALRPAAGKDEALIQDFLDEAPYLRQHSAARLALYRQEPAFRICMETR
                     RP"
     gene            41159..41656
                     /locus_tag="PLS90_00185"
     CDS             41159..41656
                     /locus_tag="PLS90_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPK00850.1"
                     /translation="MDNGQAGPREDRLPIHAVLDNLRSAFNVGSIFRTSDAGRIAALH
                     LCGMTPVPPHPRLLRTSLGAEQHVPWSHEPDPVAACRALRARGIMLVAIETQPGAAAH
                     TRFDWPRPVALVFGHEVRGIRPDVLGCCDAAVRIPMLGHKNTLNVATAYGVVLYEILR
                     QWRIV"
     gene            41765..42415
                     /locus_tag="PLS90_00190"
     CDS             41765..42415
                     /locus_tag="PLS90_00190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK00851.1"
                     /translation="MRKRRIILAYHNPVIKFYCLVRFIIIRINILEKFSEPLRQFTSG
                     HVLDVGCGFGLFAIYNATRHPGLTIHGVDYNRRRIAEALAVSRRLRVDNVRFRVGDVR
                     RRDPEVRHFKVIYMLDILHHIPVVSKYRLLRKCHRLLEDDGLLVIKDIDTGNRLKVAF
                     TWLLDKLMGPVDAVSYQSSLKLQRMLEYCGFEVTRRELIDDILPYPHMVLLCRKRA"
     gene            42518..43849
                     /locus_tag="PLS90_00195"
     CDS             42518..43849
                     /locus_tag="PLS90_00195"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPK00852.1"
                     /translation="MGAARAPIVVVLADAAERDAVRYGLDRLRELECGFDVALVPAAA
                     DPRLAMDLARQAEEEGRCVVLAGGERLGWLPDVLAAACRLPVVAIGPPGAAPAGSQAA
                     WVREPVPAALMAARIAAVADPALARRLGEDAAAGPRPSAPPLEPAAPASPRAEPWDED
                     ASAEDEDEAWKRGWRTARPHPEEPAEEGEERVEIRPVDWNRPLKGSVVGGAHRPSREE
                     EAPPAPPPPRRLGRIAIEPESPELDVIEEVVDCLLEGGVVALPTDTVYGLAVDATNPA
                     AVERLFELKGRGREKPIVLMVDSPKLLGQVARNLTVAVRRLMEAFWPGPLTLILQKRE
                     GNFTHISPGPTIGVRLPDHSTPLTIIQALARPIACTSANPSGAAEARSADEVEDYFGD
                     ELSAILDAGPLAESLPSTVLDVTAEPFQIRRLGALGREQLAAVVGDVLEGD"
     gene            44045..44452
                     /locus_tag="PLS90_00200"
     CDS             44045..44452
                     /locus_tag="PLS90_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00853.1"
                     /translation="MRPLCVLTLALAAGLALTGCVSWPGGTDLAAVASRSGMSNVTTG
                     DGTFENYTATGHYTGMDVGIAVGIPFLTKLFEIFPMRSNEELLGDVAQTAAGDGADAM
                     INVTPAASVFTGFPFGILGIYVDRAEGTGIRSR"
     gene            44575..45342
                     /gene="trmD"
                     /locus_tag="PLS90_00205"
     CDS             44575..45342
                     /gene="trmD"
                     /locus_tag="PLS90_00205"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPK00854.1"
                     /translation="MRIDVITLFPEFFGEPLRTSVVGRAVAAGRLAVHLHNPRDWTTD
                     KHRCTDDAPYGGGGGMVMLAEPLLRAIRGVRGAAEAESVLRPPVIHLSPQGERLTQPL
                     VRELASLAGIILLCGNYEGIDERVIELEVDREISIGDYVLTGGELPALVVINAMARHV
                     EGVLGNPSSAERDSFEDGLLDFPHYTRPETVEGLAVPPVLLSGHHARIEAWRRQRQLE
                     RTARRRPDLLARAALTDEERRWLAQQGLWPPKGDAPC"
     gene            45336..45899
                     /locus_tag="PLS90_00210"
     CDS             45336..45899
                     /locus_tag="PLS90_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPK00855.1"
                     /translation="MLMVALVHYPTINKEGRVVTTSVTNFDIHDIARASTTFGVARYY
                     LVTPVELQQQFVRRIVHHWLEGSGAEYNPTRKEALTAVEIAPDLAAVGEAIERDFGRA
                     ATWVATSARRYGHTISCAALRRRLRDEPDQPVCLIFGTGSGLHPQILEEADLILEPIQ
                     GPTAYNHLSVRSAVSIYLDRLLAVRGD"
     gene            46039..47145
                     /locus_tag="PLS90_00215"
     CDS             46039..47145
                     /locus_tag="PLS90_00215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK00856.1"
                     /translation="MNSLMKTAALALGLALWSPVLMAQPAQEGPQPPAAAAPAEASKV
                     VLKVNGQEITAAQFNRVLQQMRAQPSPPMAQQEPPTMEEAQAELERQARERLIGQALF
                     TQALEQKSPTIPDAEVDARLSQFEQEVAASGMQIDDMLEQAGKTREDLRQELRQDLAV
                     QALVEEHMGDQDPSEEELRQLFDQVADQLNRPEQIQTRQIFVAFTDDGTTPTEERKQT
                     ARQRADEAFKRLKAGEDFVKVFDELSTPMPAAGEGGEFPFHARGELPPPLDDAAWALK
                     PGELSEVLESEYGFHIIQLEEHREAAPASFEEHRDALARHQLQQNIMINVPLTALELR
                     KTAKIEMLDETTGEFVPAPAAAEPTAEGEPVNEN"
     gene            47302..48300
                     /locus_tag="PLS90_00220"
     CDS             47302..48300
                     /locus_tag="PLS90_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPK00857.1"
                     /translation="MAWPERWFPFVLLAVLGAASVEAGEDAGTSATLLPAAPPAVELD
                     VKYNISPEELNQILAAIPAAPRAEPRRPRRLLVFSRAAGFYHDAIPHGAQLFAHLGAR
                     TGAFAAVVTDDPAVFEPDSLSRFDAVLMNNTTGEIFRPGNYAELDDAARAAADRTAAR
                     YRTSLAQFVARGGGLAGIHAATDTLYEWPEYGEMIGGYFAGHPWNEEVGVTVVEPDHP
                     LTAAFAGDFTIADEIYQFREPYSRGKVRVLLALDPARTDMTRPGINRDDLDFPISWIR
                     NYGAGRVFYGSLGHRRVIFWHPAVVQFYLDGIQFVLGDLDADADPVPGQSEPSEHP"
     gene            48325..50247
                     /locus_tag="PLS90_00225"
     CDS             48325..50247
                     /locus_tag="PLS90_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00858.1"
                     /translation="MRLPFFPLFLFVVPLVLLSCARLEEPTHYPTLSEPVRAVLLQLP
                     EADVQQRVDLRIAPELEQQLAALRTEQIRELIDYIVIGDVNQDLRPRSAIHALVLLAR
                     PPAPALRRAQLAGAIGDAVADGRVSRVTREFLVGQLRFLGGREAVDVLARALQDGELA
                     EPAIVALLEMEEGLIGEHERLETLQTAYRRSHGSRRATLLRALGRLGDRSLLREFRQD
                     AESTNIEIREAALDAIAAAGDPADGSRLLHALARATPATRLQAENRYVIFIDRIGRDD
                     PEAGINLSREFFRRAYLDQRGQAAAGALRVLADLQGPDALDQIGAALQSHDPDLRAAA
                     LEIAARMPGQDVTRYWVEIVNNLEEAELRATILHLLAARGDRLALACVERSQRDPDPA
                     VRQAAAHAASRLHGETAIALVDQAGATEIPITQPDLKQTLSARLQTEPDPERRTVVLL
                     DELARATQPGEKGTILGLLAREGGDAALSAVRGALIDPDPAVREAATRALAEWPDARP
                     TPDLLAVARGEDEERLRLIALRGAVRLVDRDARLPAASKLAWYDEGMRIAWRAEERHQ
                     ILASMQQVKDLAALRRTAQWLDDPATATQAATAALRIAAELSPSERAEARQTLERIAR
                     MEDPELRREALRLQGR"
     gene            50627..52684
                     /locus_tag="PLS90_00230"
     CDS             50627..52684
                     /locus_tag="PLS90_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPK00859.1"
                     /translation="MAEHTREADEKIPSNWNQKPHPAAPDDEEMPASMGGSALFAEEE
                     DDYLDPDEVPAAASEEMDAMIDQYLDQMTGALSQGQLLTVPVVAIKSDHVLVDIGEKS
                     EGIIHIREFPLRGNVPDVQLGQEIEVVVKGYDAESGLVNLSYQEARRRKALEEVNAAL
                     EQGRPIKGTVTRTVKGGLILDIGTTAFLPASQIDLRRVENFEEWIGRELEALVIEHVP
                     EKRRIIVSRRRLLEREREQMRQQAMSRLEVGSEVDVVVKRIVDFGVFVDLGGVDGLIP
                     RSEVSWQRNARPEDYFNVGDTIRVKVIEIDQETGKITLSRRLAMADPWETAVAKYPAG
                     SEIRGTVVSLTNYGAFVRLEEGLDGMIHISDMAWDAAGHKPGDYVQAGQEVSAQVLNV
                     DREGRRISLGLKQMTRDPWEDVVERFPRGTHIKGPVTGLTKYGAFVELEPGIEGMIHV
                     SDFSWDKRVSQPKDVVHRGEEIEAVVLDIDRERRRISLGVKQLSESPLALFKARHKVG
                     DIVEGEVTGITEFGAFVRLADGVDGFLHVSQIDRDRVEKVADHIKVGDTIRTKIVKID
                     SGTGKISLSRRQMMKEQEKKVIQSYLKRSGREESINPMGELLEDINLLDDLPSEPESP
                     QPREEAPEVPIERAQRVDRAPEENENQLADGLPEAPGSAGAPPAAGPEEEPRPTPEDN
                     AQG"
     gene            52940..53737
                     /locus_tag="PLS90_00235"
     CDS             52940..53737
                     /locus_tag="PLS90_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPK00860.1"
                     /translation="MPEPKPAPGRNLTSRLQPGRCQVGTILSLDSPEVAEIMSHCGFD
                     WFWIDMEHSALSCSDTQRLIMAIGARCPAIVRIPENDSAWFKRVLDTGCDGVLVPMVN
                     SAEAARKAVDACRYPPLGKRSIGVSRAHGYGMKFREYVDASYDSIDLIIQCEHHEAVA
                     NIESILEVEGISAILIGPYDLSGSFNRLGDVKHEEVQQAIDMARKACKAKGMPVGIFC
                     MDVKTALRLIPDGYDFIGVSLDSVILGQAARAMSEALHGEGNAWRES"
     gene            53869..55263
                     /locus_tag="PLS90_00240"
     CDS             53869..55263
                     /locus_tag="PLS90_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK00861.1"
                     /translation="MSKPTILVIDDETNLLRFFEYNIRGLGYDVVVGASGADFQRMIG
                     EREYATILLDLMLPDASGLDLLEQARKIHPELPVIMITAYGTIDRAVQAIKLGAYEFL
                     PKPIDLDKLNTIIRNAVEQYLLRREVRTLRRQLEPPREFQSMVGTSPAMLEIYNMIET
                     VAATTATVMVTGESGTGKELVARGLHNLSSRAGREFVAINCAAIPRDLLENELFGHEK
                     GSFTGATERYKGCFERADKGTLFLDEICEMDLGLQAKLLRLIQEQQFYRVGGTEPVQV
                     DVRILTATNQNPAEAVRAGRFREDLYYRLNVVPINLPPLRERREDIGQIANKYLLEFS
                     KAYGRRFQGFDLEALAALERYGWPGNVRELRNVVEQLVVLNEGERVTASMLPARILEA
                     RPAPVEPGQAAPAGGERATLTSESIRPFWQVERDQIQSALDLCGGNVQEVARRLEISP
                     ATLYRKIEKYGLVK"
     gene            55272..58034
                     /gene="glnD"
                     /locus_tag="PLS90_00245"
     CDS             55272..58034
                     /gene="glnD"
                     /locus_tag="PLS90_00245"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HPK00862.1"
                     /translation="MEPRPRRTPQPTPPVVGDYLSAEDSHEHFLKQGRQFLQDLQRWL
                     VANNHLHRSMAIIQAFTRAHDRLIQAAYQHACRQWSEETGLMPPRVAVLAIGGYGRRT
                     LMLRSDIDLMVLMAEHTGDNERFVKSLMLTLIDLKLNLGYATRTQADCLAALGNDLDS
                     ATAMTESRLLAGHRPLFNEFMAALAAALQGRWRRWFIRSVHQQMLERRRKYESTVYML
                     EPNLKEGNGGLRDAHTVMWILFALTGSLKLRQLIALARFTEEDFRRLREALTMLRVIR
                     NEIHITAESKTDQLIFTCQPLIAERLGYQSTDIRSAEEVFMADYYRHARTVERLTHRA
                     VRVLLHSDRSVWDGVVGSLKRRRLDRYHFVQDNVLFIDDAHQDYLEQEPRRILELFER
                     AAKWGYRLSDRTLDRITRAAAGLGPGFRLDPANHAAFMRLLRGPFNVHRALADMHETG
                     VLAKFLPEFERLRYMVRIDHYHHYTVDEHTFRALEEAERLQRMGSGSGDHVAEVAASI
                     KRWDLLNLALLLHDIGKGYGRGHALRGGHIAQRVGDRMGLKRADTDLVRFLVLSHLKL
                     THASQRRDLSDPAVARALAEEIGSLERLKMLYVHTVCDLRAVSPDAWNDWKGQLLAEC
                     YARTAEMLAGERRAAGGAQPELPRLKREILALLLDGQARKAGARRAGDPELERDLDEF
                     LGHVSQRYIRNMSPRQIGRHFLLRRQLQGEQRVAWELDPRPEGGYNEILVAAHDTPGL
                     FSNICGALAAKGISIWSAEIFSTNDGYALNQFRVTDLDNRPLAGGLRLDRLRDDLDDV
                     LQERKTIFELIAKHRTRRRRKAVAKAVYPTSVRFDNDSSQHHTVIEIRSTDRPGLLFW
                     ITRALNASRLDIHRSIIATEAYGVVDVFYVTDLEYNKIHDRHAQEKIRATILDHVEQA
                     AEMFAG"
     gene            58890..59453
                     /gene="infC"
                     /locus_tag="PLS90_00250"
     CDS             58890..59453
                     /gene="infC"
                     /locus_tag="PLS90_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011712060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPK00863.1"
                     /translation="MRTNTRTNEEIRVKEVRLIDEAGKQLGIMPIDKALERAQAKALD
                     LVEVAPDAQPPVCRIMDFTKYLYEQKRKQKLARKKTARVDTKEVKLRPNIDPHDFGIK
                     LNHSREFLEKGNKVKFTIRYRPREMRHYEIGLKVMDRLVEELKELASVESTTRNQSGL
                     RMQTMILAPRKEAIARRDHEEGEAAEN"
     gene            59517..59711
                     /gene="rpmI"
                     /locus_tag="PLS90_00255"
     CDS             59517..59711
                     /gene="rpmI"
                     /locus_tag="PLS90_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPK00864.1"
                     /translation="MPKIKTKGGVKKRLKPTGGGHIKRKRCNHGHILTKKSAKRKRRL
                     RQTTLVHEADERRMKKLLHI"
     gene            59881..60246
                     /gene="rplT"
                     /locus_tag="PLS90_00260"
     CDS             59881..60246
                     /gene="rplT"
                     /locus_tag="PLS90_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPK00865.1"
                     /translation="MPRVKNSVATRRRRKKILKLAKGAQGSRSKLFKNAKETVKRGMN
                     YAYRDRRVRKREFRALWIARISAGVRAHGMKYSEFMHGMKLAGIELDRKVLADLAMND
                     AEAFGAIVSQVRNSLAQAA"
     gene            60246..61268
                     /locus_tag="PLS90_00265"
     CDS             60246..61268
                     /locus_tag="PLS90_00265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010497043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPK00866.1"
                     /translation="MMAATGVETLRERLRELGEQARRQIEAAADAQALEALRVQYLGK
                     KSEFSGLLKALGQLGAEDRKLVGMVANEVKTELGDLITRRREAFDEAERQRRLAEERL
                     DVTLPGRPVPRGHLHPVTQGIREICGIFVQMGFEVATGPDIEDDYHNFEALNFPPNHP
                     ARDMQDTFYMREGGLLRTHTSPVQIRTMRERKPPLAVVAPGHTYRCDADSTHLPMFTQ
                     IEGFIVDENIRMSDLKGVLQRLIHAYFGADRTYRMRPSFFPFTEPSAEVDIRWENAAG
                     EQVWFEVLGAGMIHPAVLENVGYDPQRVSGFAFGLGVERFVMLKHGIKNIKHLYENDL
                     RFLRQF"
     gene            61373..63754
                     /gene="pheT"
                     /locus_tag="PLS90_00270"
     CDS             61373..63754
                     /gene="pheT"
                     /locus_tag="PLS90_00270"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPK00867.1"
                     /translation="MKISVRWLRQFLNVELSIAELKETMEMAGHEVEGAWDLGLESGK
                     LVVARVLDVQLHPNADRLRVCQVDAGTGQPLSVVCGAPNVTAGMNVVCALPGARLPDG
                     NTIKKSKIRGQLSEGMLCSARELEMGTDHSGIMPLPEDLEIGQPYDYIIDIKVTPNRP
                     DCLSILGMARDIGAALGKKVFPPTPRFKETLEHIDSFVRLSVKDRGACPRYTCRLIRG
                     VKVGESPLWLKRALEACGMRSINNVVDVTNYVLMELGHPLHAFDFDRLHNSEIQVRRA
                     EACERLVLIDDREITLDPEDLVIADADRPLALAGVMGGRDSEVGPGTTTILLESAYFD
                     PATIRRTARKYNFQTESSYRFERGTDRARLTLSLNRATQLIHELAGGEVIKNMLDAQT
                     TITDPGPIVLDVRRVNRILGLELTSTEIADYLVNLGCEIRRGDKESLVVSIPSHRVDI
                     SRDIDLIEEVARLHNYNEIPATMPKLIPTIRPCDRLGELATRLSDQLVALGFCEVLQF
                     SFISEADAAAGGFNIERLPRLANPLTVDQALMRPSLATGLLRVIAANQKVGEASQLLF
                     EIGKAWREDARAADPAGERHELALAAAGPVPTHWGAPAREYDFYDLKGVVENLFSWIN
                     GPAPSFARDAGNPLLHPGRSARIEWDGRPVGVLGQVHPRAVAQFDLRGDAVLALIDLS
                     LLVSLLGESVVQFKPIPKFPGSLRDLALVVDEQLPAAEILSLIHEAGRPLVDEVQVFD
                     LYSGSHVPAGKKSLALRLRLLSPEKTLTEQEITETIERIVRRLEQQFGATLRT"
     gene            63839..64741
                     /locus_tag="PLS90_00275"
     CDS             63839..64741
                     /locus_tag="PLS90_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00868.1"
                     /translation="MPSFSPRIMRRRLEAFQRGVHRLIAERGLNTQEEIQAFLEQHST
                     DGSLERMIDELDLMPEEEALELVSEAMECEDLDEMLDLLDAALEADPECIEALCLLAR
                     INCEEDSEALVDALEDIVKLGEEKLGAKMFEECRGQFWGLLETRPYMRARQELITALA
                     ICGRFEEAAAHGEAMLELNEDDNQGIRSELLVHYLLLGNLEGARALLKRYEEVTAVFA
                     WAHVFERFLAGNLEEARSAMTEARRVNPYVKEYLTGKAALPDLLPAYSTPGDRSEAIG
                     CAAMLGRVLLQRPELHTWLVNSLK"
     gene            64791..66377
                     /gene="gltX"
                     /locus_tag="PLS90_00280"
     CDS             64791..66377
                     /gene="gltX"
                     /locus_tag="PLS90_00280"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007111126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPK00869.1"
                     /translation="MHHPTIRTRIAPSPTGDPHIGTAYIALFNYVFARRHGGRFLLRI
                     EDTDRVRSTPESERAILAALRWIGLAWDEGPDVGGPHGPYRQSERTEIYRAHCERLID
                     SEHAYYCFATPEQLDAYRRERLQAGKATGYDGSFGMWTRAEARARIAAGDPHVIRLRA
                     PAEGECVIHDRLRGAIRIPWENVDHQVLLKADGHPTYHLASVVDDHLMEITHVIRGEE
                     WINSAPKHKLLYEAFGWEMPELIHMPLLRNPDRSKLSKRKNPTSIDYYRQAGYLPEAL
                     LNYLGLMAWTYPPAPGETEGPEMFSLEEMIAAFDIDRVSLGGPIFDPRKLNWLNGRYL
                     REKHDPAALLARLRDWMLNDGVWLRALPLVQPRAEKLSDVVPMTAFLFADRPAYDPHS
                     LIQKKLDGDAAARLLRIVLWELERVTPWNKETTKAAISRIAQIEGLKLRELMPVLFVA
                     LTGAPASLPLFDSMEILGAAMSRRRLVYALEALGSIGHELSSRQLKAIEQEYREKYLE
                     QGKGETQAAGDGADGSGAEE"
     gene            66446..>67367
                     /locus_tag="PLS90_00285"
     CDS             66446..>67367
                     /locus_tag="PLS90_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HPK00870.1"
                     /translation="MAATHLHFDCTFGISGDMTVGALVDLGVALEAIADRLRDLPIGP
                     FHLRAEPLLRQGIRGTRVHVEVAEDEKPHRHLRHVLDILGAASLSAAARRRAELAYRR
                     LAEAEAHVHGSTADKIHFHEVGAKDAIVDVAAAMVAVELLGAESFSAGPIYVGSGTIQ
                     CQHGVMPVPAPATAELLRALPHAAGPFTGEMVTPTGAAILRALLEDAGEPPIGGQEVE
                     SPARVVQRIGYGAGTRELPGASNYLRVLLCESPRAAAGGEEGDALPLHRHQIVVLETE
                     IDDMAPETAGYVMERLLAREALDVQFSPVQM"
BASE COUNT        10910 a        21956 c        23863 g        10538 t
ORIGIN      
        1 cgggttgcgg aatgggggga cgttgggttt ccggcgcggg cggggcgggg ggcgcggtcg
       61 ggcggcgggc cggcgtagcg ggcggggtgc gtcggccgcc gccggccaga ttggcctgga
      121 gcgaatcggc cagcttgtcg cgcaaatcct ggcggaaggg ggcgcggcgg acggcttcgc
      181 gcaacagggg cgtggcgcgg gcaaaatcgc gggcgcgcag cgcggcctcg gcgtgcgtca
      241 ccagcgtttc gaattcgacc cgatgcagct cgcgcgaggt gaactccggt tgcacgtgac
      301 tcggtcgctc cgtgatgaga tggcgaggcg gcccaccgcc gatccaggcg ccgccaggcg
      361 ccctggcaac accagacttc cacgatagcg ggttggttgg ggaaatgtca tgggaatccg
      421 ggcggaacga ggcggaacgg gtggacgagg gggacgggag gaaaactgac cgaccatgga
      481 gaacgagagc cgaggacgag agccggaagc cgggagccga attttcgggt gggtctttgg
      541 gggcgcttac gaggacgaaa gacgagagac gagggacgtg aattcgatta tgggacgaaa
      601 tttgcggggg attattccac gcggacgagg atgacggaaa tgttatcctg gccgccggct
      661 tcgttggcga gctcgacgag ccggccgcat aaagcctgca aatcttcctg ggggtgcgtg
      721 gagaggacct gatgcagggt ctgatcgtcg accatgccgg tcaggccgtc ggagcagatg
      781 aggaacaggt cgccgcattg catgtcgacg cttttgagat cgacctcgac atcctggcga
      841 ttgcccagag cgcgggtgat gacgttgcgc cagcggtggt tgcgagcctc atcctcggtg
      901 atgatgttgc gtttgagctg ttcgttgacc caggaatggt cgtcggtgag gagctgcaac
      961 tgatcgccgc ggaggcgata gacgcgcgaa tcgccgacat ggccgatgtg gatcacgcgc
     1021 tcgggggcgt agatcgcggc gacggtggtg cccatgccgc tgtaggtctc gttctcgccg
     1081 gccagccggc agacgcgccg attggcgagg tggatgccgg tcagcaggac gttttcgccg
     1141 aggctgaact tgctgtcgat gccgaagggc caggtgaaat cgcggttgat ggcggcgtgg
     1201 gcgacgaatt cgtggagggt ttcgatggcg gtgcggctgg cgatttcgcc ggcggcgtgc
     1261 ccgcccatgc cgtcggcgat ggcgaacagg cgcaggtttt cgtcgagctt gatactgtct
     1321 tcgttcgcct ggcgtttgag gccgacatcg gtgcgcccgc aggcgcggag gacggggtgc
     1381 tgaccattgc cggaatgcat gaaaacgaga ttcgagaggg cctcgcaggg gtgatccgaa
     1441 ccggtggaag ccggcccccg ccatggcggg cattcctgcg ctattggagc atagggcatg
     1501 caggcgggca agaaaattac cccccgggcg cggcgggggt ggcgggcggg gtttggggcg
     1561 ccgatcgggg ggctgatctg ggagccgatg ggacgcatgg ggcaggggaa ggattcagtt
     1621 ttcgagagcc gggagccgag tgtttgagcg gccgtttggg gggaggacga gggacaaagt
     1681 cgaggacgag ggacgagttt atcgggacga gatgacaccg ctggggatgc gggtggaatc
     1741 gtcgagcgta tcgagcacga gggcgagtcg ttcgagcggg aattcgaggt cgagcgggcg
     1801 gctgaaggcc gggatccagc cggtgcccga gcggccgaag agggcggagg tctcgcgcac
     1861 gagccgccgg acggactcat cggagtcggc gcgggtaatg cgatcgatgt cgagcccgcc
     1921 gaagtaggcg atccgccctt cgatgcggtc gaggagttgg tccagtccgc cggcgatcgg
     1981 atcgcagggg ccgatggcgt cgacgccgag ttcgaggagg tcggggagga tggcgctgaa
     2041 atcgccctcg cactggaaaa agacatgctt gccggcggcc ttgaacgggg tgataagctg
     2101 ggcgaggcgc ggcttgaaca gcaggcgcca ggcgtcgggc gagaggacca tgccgaaggc
     2161 ggcggcgaaa ttgtcgcgca gatagaggcc gtcgatgccc agttcgatca ggcgctgggc
     2221 ggcgccgagt tgaacgccga cgatgtgctc gagcaggtcg cggatgaacg cctgattgag
     2281 catcaggtcg cgcagggtgt tttcgaaacc gcgcagcgac cagcagcgtt cgaagaggcc
     2341 gaggccgagg cgggcgagga caaagcggcg gccggcgttg gccgcgatgc gcgcgcgggc
     2401 gtgcaggtag cgattgggat gttccaggtc gggccagtcg taggcgggca gggtctcgat
     2461 gtgggcgagg gggggcgtat gatcgagctg gtggccctcg cgttgatacc agtagacgcc
     2521 ccattcgtcc tcgtaaatga gcgagtcggc ggggaaggag cgttcggcgg aaacgaggat
     2581 gaaatggtgg aagagggcat cgtcgaggcg atcggcgggg acgccgagga gggcggcgag
     2641 gtggggacgg agggcgcggc ggtaattgag ctgagcgggg aagcggtcga cgggttgctg
     2701 gtcgaggatc cgcaggaccc gctggcgagg caggagctga gccatgggga gacacccgac
     2761 gggaggatgg cggcggcggg ccgagaacct tgttgaaaat ttaacttaaa aacctttttt
     2821 gaagcaagga ctttcatgac tacaatgcac gcatggaccg cgaaagggca tggcagcctg
     2881 acgtggacgg gacgagggta acagacgagg gtaacatcgg tttgcagaga tggcggatta
     2941 tcgagagccg ggagccgaat cttggggcgg gccttcgggg gcgatggcga tggcgaggac
     3001 gaggacgggg ggcggttgtt aaggaggggg gggaatgaag gaggggttca tgaaccgtga
     3061 gaacgccctg gaacggctgc agagcgaagc ggcgccgtgg gatctggtgg tgatcggggg
     3121 cggcgcgacg gggctggggt gcgcattgga cgcggcaacg cgcggctatc gcgtggcgct
     3181 gctcgagcag gccgatttcg cgaagggcac gtcgagccga tcgaccaagc tgatccacgg
     3241 cggggtgcgc tatctgcagc ggggcgagct cgggctggtg cgggaggcgc tgcgcgagcg
     3301 cgggcggctg ctgcgcaatg cgccgcatct ggtgcggccg ctccagtttc tcgtgccgtg
     3361 ttaccaccac tgggaactgc cgtattaccg ggcggggctg ctggcgtatg atctgctcgc
     3421 ggcagggcgc ggcgtcggcc ggtcgcgctg gcggggggcg gctgcgtgcg gcgcgctggc
     3481 cccgggcttg cgccgggagg ggctgcgggg gggcatgacg ttctgggacg cgcaattcga
     3541 cgacgcgcgg ctggccgtcg cgctggcgcg cagcgcggcg cgagcggggg cgacggtgct
     3601 gaatcacgcg gccgtgatcg ggttcacgcg gcgggcggga cgcctgggcg gggtcgcggc
     3661 gcgcgatcaa ctgacggggc gcgaattcga ggtcgcggcg cgcggcgtga tcaatgcggc
     3721 cgggattttt tcggatcagg tgcgggcgct ggaggaaccg ggcgcggagc cgcggctcgc
     3781 ccccagccag ggaattcacc tggtgctgcc gcgcgaattt ctgctgggcg cgacggcgat
     3841 tctggttccg cgcaccgagg acgggcgcgt tgtgttctgc attccgtggg agggacgcgt
     3901 gctgatcggg acgacggaca ccgcggtgca ggagatcgcg ttggagccgc gcccgcgcgc
     3961 gggggaggtc gactatctgc tggagcacgc ggggcggtat ctggaacggc ggccgcggcg
     4021 cgaggaagtg ttgagctgtt tcgcggggct gcggccgctg gtgcgcacgc gcgctgcgga
     4081 gacggcgcgg ctctcgcgcg agcacgaaat ccgggtgtcg cgcgggggtc tggtgacgat
     4141 cgccgggggg aaatggacga cctaccgggc gatggcggag gaggcggtcg atcgcgcggc
     4201 cgaagtggcg ggcctggcgc ggcggcggtc gcagacgggc gacctgcggt tggtcggcgg
     4261 gtgcgagccg ggcggggccg acgatgcgct ggcgcgttac ggggatgagc gggcggcatt
     4321 ggcggagctg cagcgcgaag aggccgaatg ggcggaaccg ctggcggcgg ggttgccgtg
     4381 tttgaaggcg gagatcgtct gggccgcgcg ccacgagatg gcatgcacgg tggaggacgt
     4441 gctcgcgcgg cggacgcgat tgctgtttct ggatgcgcga gcagcggcgg aggcggcgcc
     4501 ggtcgccgcg cgcctgctgg cgcgcgagtt gggccacgac gcggcctggc aggcccgcga
     4561 agtgcgggag ttcaccgcgc tggcgcggcg ttatgatccg cgggcggagt ggtgagaggt
     4621 tattaatccg ggggcgggga gcgttcaccg gcgtgttgac tcatgggaaa acgttaccga
     4681 gcggaacctg tccaggttcc gggcgggcgc tgtatcggag cattgactca ttgccgctct
     4741 caaggcggcg tttgaggctg aagacctgcc tcagctgcgt ctcaatttga tccttcaggt
     4801 cgaagggatc aagccgtgat atttcttccc ttaatagcct ctttctgttt tgggctgtat
     4861 gcggactatc caggatgcgc gcaaggggtg tctggggcgg gtcgtagcgc ttcttgatct
     4921 tggttccctc gcgggatttg gatgccagtt tcatcaccgg caagaaggca ttgcgaaact
     4981 gatcgcacag gcggtagagc acgttgatcg gctcgaccaa ctcgtgccat ccaagccgct
     5041 catagccgag cagttggcgc acatgcgtgt agtttttctg ttccacatag gcctgatcgt
     5101 tcttgtgata ggggcggctg cgcgtgaagt ccaccggttc ctttctctgg cgcaggtagg
     5161 cccacaaatg gtggttcagg aactccgagc cgttgtcgca gtcgaagccc aggatcgtaa
     5221 agggaagcac cgtctcgacc tcggcaagct gctccaggac gccgacggct cccctgttcc
     5281 aaaccgcccg attggccgtc cacccggtcg caatgtcagt aaacgtaagg ctccagacga
     5341 agtcaccggc caggcttggc ccacagtgcg ccaccgtatc ggcctccaga taccccggcc
     5401 gctgctcgtc ccacgattgc ccgcggatcg ggatctgggt cttgagcaga ctgcccggcc
     5461 gcgtcccgca aagacccttg ggcttcagcc tgacccgctc gggcgccagc agccgatccg
     5521 ccgtggcggc gctcatcgtg agcagtcgct tgcgggtcac cgcgctgagc cgctcgcgcc
     5581 gcccgtaagc gggcagccac agcggcaaag ccgctaccaa ccgtttgcca cagggctgat
     5641 cagccgccag ccacaccgcc ttaagcacct ggcgctcgca agcgccgtag cgcacggtcg
     5701 ggcccgagcg acgcgacgga ccgggtgcca cagcgttcag cgcccggatc gcatgcttgc
     5761 gatggcaagc catcatcgcg caaaactcgt ccagcacacg ccctttgccc tgccggttca
     5821 tcagcccata gcgctcgcgc atcgccatca catactctct tctggcctcc ggacccatat
     5881 ccacttcccc cgctttcctg ggtaacatcg aatatgagtc aacgaccttc tcagtccgcc
     5941 cgcaaccccc tttccccggt aacctttaat atgagtcaat tcgcccccca aaacctcccg
     6001 gcaatttggc ttgggatctg cttcacgccg tggcagagtg gaccgcccgg caattttgcg
     6061 tcgaccgcga ccgccaatcg cgacatatgg tcctgtcgat gccggacgac gcccacccgc
     6121 gccccaacgc gccatccacg acgcccgccg cgggcggtcg ccgttcgaac ggcccgaccg
     6181 gcgcccctgg cccgactccg cgcgcggcgt cgacccccga cgccgccgac atcccccaag
     6241 gatacccgcc ccgcatgagg ccccgtctgc tcgctctgtt cgcgctcctg ctcctgccgc
     6301 tggccggttg tggccgcgtc cactatcgct attacgtccg cctggacgac cgcccgctgc
     6361 ggatggatct gccgctcgac ctcgtctacg cccatccgcg gattgaggcg atcaaggacc
     6421 ggacgctcga agtccacgtc ctgtcgcccc tcgactccgt gatggccagc acggcgaaaa
     6481 tgaccaaacc cgatgactgg gaaacctact acgagacctg gcggcgcgac ttcgagaacg
     6541 tcctggccga taagatctgg cgcacggcca tcttcagcga tgtcggcacc ccctccgacc
     6601 ccgtccccct tgagaagccc gattaccaat tgcgcgtcgc cgtcactgaa tggaacgaag
     6661 ggaatcgctg gctgcgctgg attctgggct ttggcgccgg ccgcacccgc gtccagtggg
     6721 aaggcaagct ggtcaacgcc gccaacggcg aggaactgct tctcttcgcc gacgcccgcg
     6781 aacaccccgg cggaccgtat atgatcggcg tcagcttcca tccccccaaa ggcaacgccc
     6841 tgatcgcgca ggacctggcc tgggcctcgc gggacctcgc caacgcgctg cgcgaggcga
     6901 ccggcatcga ccgcgagccg ctgggaacca acagcttcca tcgcaaatac cgctacgata
     6961 actggcagag ccaggaaatc ccgcgcccca ccgaagagat caggccgcca tcgctgcgcg
     7021 tgcgcgttgg tggggggacg taatggaccc atgggcgccg ggcctcgccg cccgcgcggc
     7081 atggcagttg cgccccgagt cctgcctggg ggcggcggcc gtcgccggct gcctgctgct
     7141 gctgggctgg ccgctcctgc cccggccggc gcgatcgtgg tgggagcaat ggctgctgcg
     7201 cgcggcggcc ggctgcgtcg ccctcccgat cctcgtgctc gtggcggggc aggtccgcgg
     7261 cctgctcttc accctcccct ggcgctggtt ggggttggcc ggctggctgg cgttcgcggc
     7321 gaccggcatc gcccggctgc gcgcgtcgcg ccggaccgcg cccgcgctcc caggcgccgg
     7381 cgcaccgccc ccctcgctct tccggcttct cgcgctcgcc gggtggatct gcgtcggcca
     7441 ctggcacctg cagatgctct ggccggcgct cagcccgccg ctcagttacg atgtgctcga
     7501 ataccatctg gcgctggtga cacatggctt cgccaccggc cggtttgagc cgatccccgg
     7561 catcctctac acgcgccagc cggtggcgac cgaggcgctc ttcacgcttg ccgccgcggc
     7621 cgaaggctgg gcccgcggcg ccgcccccgg ccttctgaac tggctgctct tcctcatggc
     7681 gacgctgctc tttgcccctc tggcccggcg tctgcggctc cccgcccctg tccggccgtg
     7741 gctctggctc gccgtcctcg ctcacccgct cgtctttaaa ctccagctcg ataacttcac
     7801 cgactggacc ggcgcgctgc tcatgctggc ggggctgctc cttgccaccg ggcggcgcgg
     7861 ccgcgcctgg gccttcccgc tcggcctgtg cgcgggcggc gccgtcgccg ccaaatggac
     7921 gaatgccgga acggtggcgc ttccgctcct ggcggtggtc gtggcgaccg cccgcgggaa
     7981 acccacctgc gggcgcgagc tggccggctt cggcgccggc gccgccgccg cgctcgcccc
     8041 ctggttgatc tggctgggcc ggaccgtcgg caatccgttt gccccctttc tcgccggtct
     8101 gtttccaacc gacgcctggt ccgccgagcg gctccagttc ctcctggcca cccatcagcc
     8161 gttttctccc ctggatgttg attactggac gaatctggtc cgcaagctgg cggtggaact
     8221 ggacgcctgg ccgatgatcg ccgtcgccct ggtgctgtcg gccggcgcgg tggccctgcg
     8281 ccggcgccgc ggcgcgcgct ggctcgccga ccgctccgcg cgaaccgccg cggcgctgct
     8341 ccttggcttg gtcttcagcc tgttgctgtg gggtcaactc cgccacgccg ccgatcgatt
     8401 cctcgccccc gtcatcgtgc tgtcggcgtt gttgctgggc gacgccctgc gcgtggttca
     8461 gcgcgcggcc ggtccccggc gcgggacatt cgtcatggtt tcctccgcag tcatccttgc
     8521 cgcgctcgcg ctctggccgc tgcggctgca actggcgacg ctgcgggcgc tcgccatgcc
     8581 ggccgtggcc atgggccgcg tgtccctgcc cgagtacctg cacaatggac ttggcgcgac
     8641 ggccgcgatg ttcgccgccg tcgaccgcct gccggccgac gcgcggctga tcgccatcca
     8701 cgaagcccgc ggctattact tccgccgtcc cgttgcgctg gcgtcggtct tcgatcggac
     8761 gccgatccgc gatgcgctgc gcggcgccga ttcggccgag tcgatccgcc ggcggctggc
     8821 cgacgccggc tatactcacg tgctggtcaa cgaattcgag caggcccggt tgctcgcgat
     8881 gcacccgccg ctgcggctgg aggacgatcc gcggcgccgg cggcttgtcg agcgcgacga
     8941 ccagacgggg ctcgtccgca cgttccccgc cgaggcggaa ttcgccgtcg atccccccgc
     9001 gccggccgag aagcaggcct atctggagtt tctcgccgcg ctgcgcgcgc ggccggtctg
     9061 gatcgaggcg ccccccgcgg gaacccccgc catctggatc gccgatttgc gcccgaccct
     9121 gccttgaggc atgccggccc ctgtgctaaa acagggcggc gcggtctccg gcctcccgtt
     9181 ccggaccgtc caaaccgacg cgcgaacaga tttccattgc ccgcggggtt tcattcggaa
     9241 tcaatatgag catggcgacc ggcagcaagg actactacaa gacgctgggg gtggagcgct
     9301 cggcttcgcc cgacgagatc aaaaaggcct accgcaaact cgcgctcaag tatcatcccg
     9361 accgcaaccc gggcgacccc gaggccgagg agcgcttcaa agaggtttcg gaggcctacg
     9421 aggttctgag cgatccccag aaacggcgaa tgtacgacgc ccacggctac gagggcgtcc
     9481 gcggcgcgtt ccgccacggc ggcttcacct gggacgactt ccaccacgcc tccgaattcc
     9541 aggatatctt cggcgacctg ttcggcaatc tgttcgggtt cggctccatg gggggcggcc
     9601 gttcgcgccg cggccggccg cgcgggcgcg acttgcgcct ccggctggat atcgacctgg
     9661 aggacgtcct gcgcggcaag accagcgaga tcaccctccg gcgcctcgaa tcctgcctca
     9721 agtgcggcgg cagcggcgct cagcccggca cgcgacccga aacctgccag cgctgcggcg
     9781 gccacggcca actgcgcgtc acccaagggt tcttcagcct caccaccacc tgcgacgtct
     9841 gcggcggcag cggccaggtc gtgcgcaatc cctgccccga atgcggcggc cagggccgcg
     9901 tcaaggaaaa ggtcacgctc aaggtcgaag tgccgcgcgg cgtggaaacc ggcacgcagc
     9961 ttcgcatgct cggcgaaggc gaagccggcc cccccggcgg cgaacgcggc gacctctaca
    10021 tcgtcctgaa cgtccgaccc cacaagcgct tcgtccgcga gggcttcgac ctgcactgcg
    10081 agctgccgat cagtttcccc caggccgcgc tcggcgacac gatctccgtg gaaaccccct
    10141 ggggcgcgca ggaactgaag atccccgcgg gaacccagcc gggcgaccgc ttccgcctgc
    10201 acaacctcgg cgttccccag cgcgatcacg acgacgccat gcgcggcgat ctgtatgtcc
    10261 acgccaagct ggtcgtgccg acccggctga ccgaccgcca gcgcgagctg ctgcgcgact
    10321 atgccgccgc ctcgggcgac gagaagttgc aggaagaccg cggattcttc gaccggttca
    10381 aacaaagcct cggcctggac gactgaacct cgccggccgg atgacgcccc tcagccggcg
    10441 gtggtgcggc gcacgaccag ccggtccccc ggcacgaacc gcaccatcgg ccgcgcctgc
    10501 ttgtcttcga tcaggtgcag cgccagttct cccgccaccc ggccgatctc ctcgcacggt
    10561 tcgctgatcg tcgtcagctc gatccccggc aaaccacccg acgtcgagtc gcccatcccg
    10621 atcacggcca cgtcctcggg aacccgcagg ccgatgtagc gcagcagcgc cagcgcatgg
    10681 agcgccatca ggtccgatgc gacgatcagg gcatcgggcc ggtccctgga ctgctcgaac
    10741 atccggcgga tcagccgctg cacgagcggc tggagccccg cctgcgactc ggccttgccc
    10801 tcgccgatcc aggctgggtt gaccggcaga tcgtgttcgc gcagcgcctc gtggaacgcg
    10861 tcgtggcgct cgcggtggac gatcgagtcg cgccgcaccg ccagatagcc gatcttcttc
    10921 cgcccgccgc cggccagatg ctccaccgcg cggcgcacgc cggcgctggc gtcccaggcc
    10981 acatagctga cctgcgggaa gtcctcaaag gcgtcgccca ggaacagcag cggcggcccg
    11041 tgacgcacga tccgctcata caggctgcgg ctgccgggca gcggaaaggt caggatggcg
    11101 tcggcccgcc gttcgagcag ggattgcacc tcgcgctgtt cgcgcttgtt gtcgccgcga
    11161 tgcagcgcgg aaaaaaccgt gaagctgtat tggtccagaa cctcctgcag accctccagc
    11221 acgcggtcgg cccagccgtg gcggaagttt tccacaatga cgccgacgac ccccgaccgg
    11281 cggatgtgca ggccgtgcgc caggcgattg gggacgtagc cggcttccgc cgccgcccgc
    11341 agcacccgct ccgccgtcgc cggcgagatc ttctgctggc gcacctggcc gttgaccacg
    11401 tacgaaacgg tcgccgtcga cagccccagc tcccgggcca gcgtggaaag ggtgacggcc
    11461 ttgcgtgaaa ccttgggcat gctgcgcgcc gccgcgcccc ggcgggcccc ccgtgaatga
    11521 tccgcgcgag acgatccgcc cctgcgggac cgttcgcccg aatgaaacca ataagtcttg
    11581 ttccggctgc ttttgcaagt caaaactgac ggaattggcc agcccttatt taccaagtgg
    11641 gtggagatcg aaatttacgg ttgcaggccg gcgccggcga cccgcgcgcc gcgttcgcga
    11701 gaacggacgc cggacgcatt ccaccgagac ctcgctactg aaccacccga cctcgaaacg
    11761 acgagcgatt ttcttgaaaa gtggcgcgaa agatgaaggg gtggaaggaa cgcgggggtt
    11821 cgcgggggat tttcatcgat caccgtacgt gcgcgtcgct caacgtacaa cgagcgtacg
    11881 ctgcggtgcg ccccggggaa cgcctgtgca agccttgcac aggccagggg ccgcccagcg
    11941 gcaaatcatg cgcgccatcc ggtgcgcaat cccaaaaaaa tgaaatatcc gcggctggaa
    12001 ccggatttgc tctgcctggg tgacgtggcg ccttgatccc gccacgcaca tctgactgca
    12061 agggagagat gcaccatgag acgaaagctc gggtggatga cactgatggc ctgttggctg
    12121 ctcctgggcg gactgccgac ggcggaggct cagccgggcg agcgtgaagg ccggttcgac
    12181 cggatggacc agaacagcga cggcgtggtg accgcggagg agttcggcgg accggccgaa
    12241 cggttcgcgc ggctcgacgc cgacggcgac ggcaagctca cgcgcgagga gatgcgctcc
    12301 agcttccagc ggcagcgcgg cgaaggctgg gaccccggcc ggtggcgcga catgatgaac
    12361 gagcggatga aggagacgct gggcgcgtcg gatgaggaat gggccgtcct gcagccgaag
    12421 atcgagaaga tcaacgaact gcggatgccg atggccaccg gaatgatgtt cggcggattc
    12481 ggcatgcggg gtcgcggcgg cgacggcgac ggcaaccgcg ggccgcgcgc cggtttcggc
    12541 ggcgagccgg ctcccgaggc cgcggcgctg caggaagcgc tcgacaacca gaacacgtcg
    12601 gccgaatccc tgcaggagaa gctcgatgcc ttccgcaagg cggcggccga tcgcgaggcc
    12661 aagctgaagg aggcccgcga ggagttgcgc gagttggtca cgccgcgcca ggaagcgctt
    12721 ctcgtcctca acggaacgct ggattgatgg cggagccatg gcggaactga gcgagcaaac
    12781 atcacccctg atgacggcgg ccgggcccga cgccatgctg gagcgcatgg tgcgggcccg
    12841 gcagctcagc gccgaggatg ccgcggcctt ccggcgccag cacgccaacg gcgccttgga
    12901 tgaagcggcg gtgctgcgct ggctggccga ggagtacggg ctggagtaca gcgacctgag
    12961 ccacgcgacc ccggaccgcc aactcctctc gcggttcccg gcgcgccacc tgctcaagga
    13021 ggaagtcctg ccgctgcgcc gcgtcggcga cgtggtcgaa gtggccacct cgcggctgtt
    13081 cgccgtcgag gggctggaca tgctgcggac gctgacgggc gagcggctgc ggccggtgct
    13141 ggcgccgtcg gcggcgatcg cgcgcgaaac gaagaagtgg ctcggcgtcg gcgccgacac
    13201 gctcgacacg ctcgaggagc aggcctcgat ccaggtgatc gacgaggagg acgacaccaa
    13261 tgtcgacctg gatgcggcgg cgcaggacgc ctcgatcatc cgcttcgtca atcaggttct
    13321 gagcgacgcc atctcgctgc gcgccaccga catccatctg gagccgttcg aggacgagat
    13381 gcggatccgt taccggatcg acggcatcct gcaggatgtg ccgctgcccg gctcgatcaa
    13441 gcgctttcag ccggcgatcg tctcgcgggt gaagatcctg agtcacctcg acatcgcgga
    13501 gaaacgcctg ccgcaggacg gccggatcaa ggtcatgctg gagcgccagg aggtcgatgt
    13561 gcgcgtttcg ctgatcccga tgctccacgg cgaaggggtc gtgatgcgtc tgttgaagaa
    13621 ggacatggcg ctgcggggcc tggcggaact gaagatgggc gcgcgcgagg cggccggctt
    13681 cgcgcgcctg ctcggtctgc cgcacgggat cgtgctggtg acggggccga ccggcagcgg
    13741 gaagtcgacg acgctctacg cggcgctcaa cgaaatcaac gacgccgagc gcaagatcat
    13801 cacgatcgag gaccccgtgg agtatcacat ccgcgggatc aatcagatcc aggtcaacga
    13861 gaagagcggg ttgtcattcg cgcgcgggct gcgctcgatc ctgcgccacg acccggacgt
    13921 gatcctgatc ggcgagatcc gtgatgcgga gaccgcgcgg atcgcggtgc aggcatcgct
    13981 gaccggccac ctggtctttt cgacgctgca taccaacgac gcggcgagcg cgccgacacg
    14041 cctgatcgac atgggagtcg aggcgtatct ggtggcggcg tcgctggagg gggtgatggc
    14101 gcagcggctg gtgcgcgtgc tctgcccggc ctgcaaggcg gtcgacgact cggcgccgac
    14161 aacggcctac aagcagcaga tcggtctgcc ggaggaaacg cccctgtacc gagcggtggg
    14221 ctgcgaggcg tgccgccgga cggggttcca gggccggcgg gcgatttttg aactgctcct
    14281 gctcgacgag cggatccgcg agctgattct gacgacgcgc tcggtggcgc gcctgcgcga
    14341 ggcggcgcgc gaggccggca tgctgacgct ggtggaggac ggccggcgcc tgctggccga
    14401 cggcgtgacg tcggtcgatg aaatcctgcg cgtgacgcgc gacgaaacgc ggaccaacgg
    14461 cgccgcggcg aaggagggct gaccgcgcca tgcccgcatt cagctaccgc gcccagcgct
    14521 ccgagggggg gattgccgag gggcatctga cggccgaaac gcgccaggag gcgctgcgtc
    14581 aactggcggg gatggggctg cgtccggtcc ggctggagtc ggccggcgag accgcggccg
    14641 agcgcgccgg cgcgccgctc gggtggacgc ttggccggcg aggggtctcg ccgcgggcgc
    14701 tggagaattt cacccgccag ttgtccagcc tgctgacggc gggcgtcccg ctcagccgcg
    14761 cgctgcggat tctcgcgcgg gaggggtcat cctcggcggc cgccgaacgc tggcgcgcga
    14821 tccacgacct ggtggtcgac ggcaacggcc tggccgacgc catggcgcgc catcccgacg
    14881 tgttcccccg gatttacgtg gcgatggtcc gctcgggcga aaccggcggc tttctcgacg
    14941 tggtactcaa tcaggtcgcc gattttcagg cgcgcgagcg ggaattgcgc ggcaaggtcc
    15001 tctcggcgct gacctacccg ctggtgctga tgctcgtcgc ggtcgcagtg ctgatcttcc
    15061 tgatgacgtt tttcatcccg cggtttcagg tgatgttcga gggattcggc gcggccctgc
    15121 cggcactgac gcgcgccatc atcgcggcga gccatttcac gacgcgctgg ggcctgctga
    15181 tcgccgtggc gctggcgctg gcgtattacg gcctgcgcct gtggctggcg acggccgagg
    15241 gccggcggcg ctggcaacag gccgtgctgc gtgtgccggc cgtcggcgag ctcgtctcgc
    15301 gcctggcgat gacccgcttc tgccggatgc tggggacgct gatcggagcg ggggtgccgc
    15361 tgattgcggc gctgcgcgtc gcccgcgagt cgctgggcaa ccaggtgctc gtcgatgccg
    15421 tcagcgacgc gatcgagcgc gtgcaacggg gcgaggcgct cgccgcgagc ctgcgcgatt
    15481 gcccgcggct cttcccgccg accgccatcg agatgatcgc ggtggcggag gaatcgggcc
    15541 agttggatcg cgaactgatc cgcctgtcga acgagacgga gaaggaactg gacgggcggc
    15601 tgcgcacggc cgtctcgctg accgaaccga tcctgctcct gctgatggcg gcgttcatcg
    15661 gcgcgatttt catcggcatg gtcctgccga ttctgtcgat tcaggattac ctgagctgag
    15721 caagatccgg atggcgccag atggctttta tgtcctttat gtcccttacg tcccttcacg
    15781 aactctcgcg cgcgggacga cattctttcg aacgaggagg ttgagatgat aatgaaactc
    15841 aggggtcggc cgacgggttt cacgctgatt gaattgctcg tggtgctgat gatcatcgcg
    15901 acgctcgcgg cgatcgtcgt gccgaaattc gccgggcgca gccgccaggc ccaggaaacc
    15961 gccgcccagg cgcagatcgc caacatctcg accgcgctgg acgccttcga ggtggataac
    16021 ggctactatc cgaagggcga cgacggcctg gacgaactgc tggagaagcc gaaggacgcc
    16081 aaaaactggc gcggcccgta tctgaaacag ggaatccccc tcgatccgtg gcagaacccg
    16141 tacatttatg aatatcccgg caagcacaat gaagacggct acgacctgat gagcatgggc
    16201 cccgatggcc gcgtgggcgg cggcgacgac atcaccaact ggagcacgaa ggactgagac
    16261 gtggagcggc gcgccccaga acgccccgac cggggctttt cgctcctgga gttggtggtg
    16321 gtgctggcgc tgctgacggt ggtggccagt ctgtcggcgc cgcggctggc cggcttcatg
    16381 cagggccgga ccgtgcgcga ggaagcgcgg cggatgctga gcatgctgga gtgggcgcgc
    16441 ggcgaggcga tcgcgcgcgg cgagcgggtc gtcgcctggt tcgatccggc gacgtcgact
    16501 tacggcgtcc ggctcgatac ccccgccggc ggggcggagc agccgctgcg ccggtatgcg
    16561 ctggacgacg atttgacgct gcacggcgag tccgcggccg cgaacgaatc gggccaactg
    16621 gaggtcctgt tctggcccga tggcgcgatc gatcccctga gcgcccgccg gattgagtgc
    16681 tgggagaaag acgcgccgtc gctcgcgatc gcgctggatg agcccggctt caacttccgc
    16741 gtggaggatc cgcgcgatgc gcccgcaagc tgaccgcccc cgcggcttca cctttatcga
    16801 gatcctcgcg gcgatgctgt tcatggcgct cgtcgtgccg gtggcgatgc agggcctgct
    16861 gctggcgaac caggtgacgc agcgcgcgac gcggatgcgc caggccggcg cgctggccga
    16921 gcgactcctc aacgaggcga tcgtcacgga atcgtggcgc gacggcgacc tggccggcgc
    16981 gtttgagcgt ccctgggaag gttatgactg gcgcatcacg agccagccgt ggcccgaaga
    17041 caccatgcgc ctcgtgacag tggaggtggg ctaccaggtt caggatcagc ggtttgtcga
    17101 gcggctgagc acgctggcga gcgagttcgc cgaggaagac gcggggttgt cggagtaatg
    17161 cgcggtattg tcattaacat ttcgcggcgc gggttgacgc tgctggagct gctggtggcg
    17221 ctggcgattt tcgcctcgct gctgacggcg ctcttctcgg cctactgggg ggggctgcgg
    17281 ctgcgcgaga aggcctggac cagcatggcc gacgacgccc ggcgcgatca tgtcggcctg
    17341 ctgctgcgga gcgacctgcg ccatctggcg gcgccggtgg gcgtgctcgc cggtccgctc
    17401 gttggcgaga gccaggggaa cgccttgcag cgcgccgatc ggctgagttt cacgtccacg
    17461 accgggctgg tgtcggacgc ggcgccctgg ggcgagctgc aacaggtcga gtatttcctc
    17521 gaatatccat tggattcggg gcaggccgag ggttttgact tcatccggcg gacgcggcgc
    17581 gacctgctcg ccacgacgat ctcggaggac gacatccccg gtcccgagtg gcgtctgctt
    17641 cacggcgtgc gcagcctgat gatccagtat ttcgacggcg aggcctgggt cgattcgtgg
    17701 gactcgacga cggtggagaa tgagaatccc gaggcgatcc ggttgtggat tgagttcgtc
    17761 gaagcggagg cgggtgcgcc gccgcggccg ccgatcgatt tgctggtcca ggtgctgagc
    17821 cagccgcgcg cgtccgcgac cgcgagtggc gcggccgcgc cggcgggtgg cgcgggcgga
    17881 ggcgcgggcg ggggtgcgac ccccgagacg cccgcgggca gcggtagagc gggcgggagc
    17941 cgtgaaggcg gcgcccgcga aggcggccca cccaacggcg gagcccgcgg ggagcgagcg
    18001 acatgaacgc gacttccccc ctgcgcaccc catcggctgg ccgccgcggc agtctgctca
    18061 tcattgtcct ctgggttgcc ctcggcctgg tcacgctggc gctctacttc ggcggcggcg
    18121 cgcggctggc ctatcaggcg agcgccaacg cgctggccgg ccggcaggcc gagcaggcga
    18181 tcgagggggc gatccgttac gcgctctttg tcctggatta ccaggtggag gagggccgcc
    18241 ggcccctgtt ggaggagggg agctatcagc ccagcgcgcc gctgggcgag gccgcgttct
    18301 ggttcatcgg gcgcggcgcg gcggatgatc tgacccagac cgagccggcc tggggactgg
    18361 tcgacgaagc aagcaagctg aatctgaata cggcgacaat tgagatgctc gaggccctgc
    18421 cgaacatgac ggccgatctg gcggcggcga tcgtcgactg gcgcgacgcc gatgaggagc
    18481 tgagcccggg cggggccgaa agcaccgatt acgagacgct cgatttcgcc tactcggcca
    18541 agaacggcga gttcgaaagc atcgaggaac tgcgcctcgt cctcggcgtc gacgagatgg
    18601 tcctctgggg cgaggaccgc aatgccaacg gcatcctgga tcccaatgaa aatgacggcg
    18661 aggcaagctg gccgcccgat aacgccgacg gccggctcga cgcgggcctg gcggaatacg
    18721 tgacggtctg gacgcgccag cccaacacgg atcccgacgg cgagccgcgc ctgaatctgc
    18781 ggggcgactc ggtgcagcag gatctgcggc aaatgctggt cgagcagttg gatgaatcga
    18841 aggccaacga cgtgatggcg cggctcggcg gtcggttggg ccagatcgaa agcccgctcg
    18901 aattcctgat cgaaagcggc ttgacgccgg aagagttcgc ccgggtggaa ccctatctga
    18961 ccgtcggcga cggcgactac cgcgtcgggc tggtcaacgt ctgcgtcgcc ccggcggcgg
    19021 tgctggcctg cctgccgggg atggacgagt cgcaggccga agcgctggtc gccgcgcggc
    19081 gggatctgga ccccgcggcg ctggagtcga tcgcctgggt ggcggcggtg ctcgaagagg
    19141 aagtcgcgcg cgaggtcggg ccgtggatca ctgccgagtc gtggcagttc agtctcgaca
    19201 tcgccgccgt gggcgcgaac ggccgcggcc tgcggcgcga gtgggtggtc gtggacaacg
    19261 aggatcaggc gcgcgtcatc taccggcgcg accgcagtcg gatgggagcg ccgttcgacc
    19321 tcagcgccgc ccgcgctcaa ttgaaaacga tcacgacgat caactgaacg gacggacacg
    19381 cgcgcgccct cgcggcgcaa agctggatga aaggctgaca tggcaaagcg aatcacagcg
    19441 ctggtggtga ttgaactgac gcgggagcga ttgcgggcgg ttgaagtgcg gccgggcgac
    19501 cgggacgcga ccgccggccc ccgcgtcgac gaggaattcc gcctgaaccc gctcagcgac
    19561 gaagccgatc tggtcgggcg ggaactccgc tcccgcctgc atgccgccgg gattcgcgcc
    19621 cgcgcggcga tcttctgccc gccgctggat cagttgctcg tccagacgat cgacctggac
    19681 ggcgtcgcgc ccgaggatct ggaatcgctg attgcgctcg aagcggagcg ggcctttccg
    19741 ttccccagcg acgagctgat cgccgcgctc tcgcggttca cgaatgcggc ggggtcggcg
    19801 cgcggctgcc tggccgtctt gccggtggcg gcggcggacc agcttgcggc gatcgcgcgc
    19861 catgccgggt tgcgcgtcgt cgggattcgg ccggccctcc cggtgacgct cgccgcgcgc
    19921 tccggcggcg ccgaagccgc gctgcgtctg accgagcacg agctgcagtt cgccgtggcg
    19981 gctcccgatg gcatcgccct gctgcgcgcg ttccagagca tgcggccgac gctcgcgcgc
    20041 gacgcggccg aggccgacgc cctcgccggg gagctccggc gcgagttccg gctgacgctg
    20101 cgccgcgtcc agaccgaact gggcgccgcg ccgcgacgga tccatctgtc gggcgccgcc
    20161 gacgactgcc gccggttcat ggcatggtat cggcgcgaat tcgccgattc ccccgaactg
    20221 gtttacgacg actcggcttc ctcgacggag cattcgatcc tcgcggccct ggcggcgacg
    20281 ggccgcgcgg cgctggcggg cgaacgcccc ggcttcgact actggcggcc ggccgagggg
    20341 cgctggcaaa ccggcttgcg ccgctggcgc gcgcgcggcg tgatctggcg cgccgccgcc
    20401 gttgccctgg cgctgggcgc gatcgccgtc gcggcgatgg cctggcacgt ctggagcctg
    20461 cgacggctcg agcggcgctg ggcggccatc gcgccgcgcg tcgaacgcct ggcggcgatg
    20521 caggaggggc tccgtgcgcg ccgcgcctgg ctctccgcgg agcccgagca gctcgctctg
    20581 ctccatacgc tgaccggcgc gttccccgaa accggcagcg tctgggcgcg caccctggtc
    20641 atccgcaaca gcgagcagac ggtgcagtgc gtcggcctgg cgcgcagcaa tcgcgattgc
    20701 ctcgccgtgt tcgaagcgct ccgccagacc gcgggagtcc aggacttgca ggtggtccag
    20761 gtggaagggg gcgacccaat ccagttcacg ctgagctatc attggcagcc ggagggggcg
    20821 cgatgagccg gatcccgcgg acgaacctgc tgttgatcgt cggcgcggtg tgcatcgccg
    20881 cgttcctcgg cgaccggctg atcctgacgc cgcagcttgc cgcgtggcgc agccgcgccc
    20941 agcggatcga acaactggaa cgcgacctgg ccaacggcga gtacctgatc gaacgggaag
    21001 aagcgatcga ggagcaatgg gccgaggcga tggattgggc gctgcccgcc gaccgcgccc
    21061 aggccgaggc cgagctgctg caagccctca atcgctgggc gggcgacgcc gccttgcggc
    21121 tgagcgacct gaaaccgcgc tggcgggagg acgagcaatt gggcgagcaa ctggaggtcc
    21181 ggctgaacgc gcagggggac ctggaggata tcagccgatt tctctatgcg ctggagacgt
    21241 cgccgctgag cctgcgcgtc gaaggggtcg agctgcgcgc gcgcgacgac cgcggccggg
    21301 agatcacgct cgacctgcgc atctcggggc tgacgcggtc ggcggagccc cgcccggagg
    21361 cggcctcatg agacgcctgt tcgtgttgct catcgcctgc ctggccgccc cgccggcggt
    21421 tttcctccgc gcgcaatcgg attcggatga acgcaacgca gagggtgaac cgccgggttt
    21481 cgaggcgtat gcgctgatcc tcgaacggaa catcttcgat ccgcagcgcc ggccgcgcgc
    21541 gcccgagagg ccggaagagg cgccgcgcga cgcgcctccg ccccccccgc cgcgggtcga
    21601 gtcgatcgag ctgctgggaa cgtggattac ggagcaacag gcgctgctct ttaccgaagc
    21661 caaccgcgac gaataccgcg gcacgctgga tgtgggaagc cggttgggcg agttccgcat
    21721 cgccgcggtg gagccgggct tcgccgtgct cgagggcgac ggggagcggc tgacgtggcc
    21781 ggtgggctac cggctggagc ggcgcgagga cgaaccgctc tggcggctgg cgggcagggc
    21841 gtccggcgtc gcgcgcgacc ggccgacgac cgcgaccacc tcctcgagcc gatcctcggc
    21901 gagcagttca agcggcggtg aagccgccgg cgatatgatc caacgcatgc gtgaacgacg
    21961 cctgcgggaa cagggagcac gatgatcatg aacaccatcc acctcgcaat cctgagcctc
    22021 gccgtgtggg cggccgcggc ggccccgcgt gcgcagcaac cgttgccgat cgacgtcgag
    22081 ccgccggccc cggtcgagga actgcgcctc aatttcaagg gggttgacct cgataccgtg
    22141 ctggattacc tgagccgcgc cgccgggttc atcgtcgtca aggaagtgga cgtggcgggc
    22201 accgtggacg tcatcagcca gcagccgctc agtcccgatg aagcgctcga ggtgctcaac
    22261 acggtgctcc atgggaaagg ttacgcggcg atccgcagcg gccggatcct gaccatcgtc
    22321 gaccgcgacg acgcgcgcgc gcgcgacatc cccgtccgcc agggcaacaa tcccgacgcg
    22381 atcgagcgca gcgaccagat ggtgacgcag atcatcccgg tccgctacgc gaaggcgacc
    22441 gaactggtcg aggacgtgca gccgctgctc ccggaatggg cgacgctgac ggcgaacgaa
    22501 agcagcaatg ccctcgtcct gaccgatacc cagaccggcg tgcggcggat cgcggaaatc
    22561 atccgcgcgc tcgatcagtc gatctcggag atcagcgccg tgcgcgtgtt caacctgcgc
    22621 tatgccgacg ccaaggaagt cgcgacgatg atccagacgc tctatcaaca gcagcaggca
    22681 ggcgcgaacg aaggctcgcg gcggcgcgaa gtattccagc gctttcgcgg accatggggc
    22741 ggcggcggcg gaggcggcgg cgatgccgat caggagcagg ccgccagcgc cgcgctgcag
    22801 gccggcttga aggtggtcgc ggtcgccgac gagcgcacca attcgatcat tgtcagcgcg
    22861 ccggaggatc tgatcgacac gattgagtcg ctcgtcgccg cgatcgaccg ttcgaccgat
    22921 attctgactg aggtgcgcgt cttcccgctg cgctattcca atgccgaaca gatggtcgag
    22981 gtgatcgagg ggatcttcgc cgaggaaagc gagaatcaga atcaaccgca gcagcgccgg
    23041 ttccgcggtc cgggcggctt ctggggcaac tgggggcgcg gcggtggcgg tggcggtggc
    23101 ggcggcggca ataacaccca ggagcagagc gagcgccggg tggccgagaa caccgtcctg
    23161 gcggtggccg atacgcgcac caattcggtg gtcgtcagcg ccgtgagcgc cgtcatggat
    23221 caggtcgaac aggtgatcgc cgagctggac cgcgacccgg cgcgcaacaa gcaggtcttc
    23281 gtctattcgg tccagaacgc cagcccggag gacgtggcga tcctgctgca ggagatgttc
    23341 ggcggcggcg ggggatcctc cctccggcgc agcaccaacc agaacacggg cggcgccaat
    23401 ccgaatccga ccccgagcct gggcggcggc ggcggactcg gtggaaacat gaatcgcaac
    23461 cgctaggacg gcccgtcgga atcaatcgga aaagagagag cacgcgatga atgcaaggct
    23521 tggaacatgg atcggatgcg cgctggcgct gctggcggcg ggaggcggcg cgtgggcgca
    23581 ggagatgggt gcgccgcccc ccgccttccc gccgccggat ttcgccatgc cggcggccga
    23641 ggccccgccc ccgcgcgaca cgctcggcac cgaactggcc gacgatgtga tcgtccagta
    23701 cgatcccgag accgactcga tcatcgtgat caccgaccag gctacccatc agcagatcca
    23761 gcaggtgatc gaaacgctcg accggccggt gccgcaggtc ttgatcaagg tgctgttcct
    23821 cgaggtgacg cacagcaacg acctcgatct gggcgtcgaa gccgcctata tcgacaacga
    23881 cgagaacgac gcggccgatt ccgtgatgac cgatttcggc gtcgacgccg ccgcgctcgg
    23941 cgggttctac cgcatcctcg atgaggatct gcgcgtcacg atgcgggcgc tggccagcgt
    24001 cggcaaactc gaggtgctgt cgcggccctc ggtcctcgcg cgcaacaacg aaacggccac
    24061 catcaccatc gggcaggaag tcccgttcat tcgcaacacc cggatcaccg acaacggcca
    24121 gacgatcaat accattgaat atgaagatat tgggattatc ctgcaggtga cgccgcacat
    24181 cacgccggaa cggctggtcg agatggaagt gctgccggaa atctcgaccc tgacggcgga
    24241 cacggtgccg atttcggaaa cggtcaacgc gcgtgtgatc gccaagcgct cggcgcagac
    24301 ccgcgtcgtc gtcgccgacg gcaagacggt ggtgatcggc gggctgatgg aagatcagga
    24361 gaccgagtcg gtcaacaagg tgccgattct cggggacatc ccgctgttgg gggcattttt
    24421 ccggcgcacc gtgaaggaaa agaccaagac cgagttgctg atcttcctga ccccctacgt
    24481 ggtggacggc caggatgcgc tcgaccgact gaccgtggcg gagcatcaac ggacggaaat
    24541 caccccgcac gcgctgaccg agccgggcgt gcgcgccaag tacctggaag gcctgccgcc
    24601 gagaatcgaa acggcgccct gaatggatcg actctatcgc aatccccccg ggtggatcgt
    24661 ctactcgacg ctggcgctct gctggctgct gatcgtcgtg tggctggctt tcgagcatgc
    24721 gcgggtgcgg cggatggagc gcgaggcgct gctcaaccgc gcgcgcgaca tctccaatac
    24781 gattgcgctc gtgacgcgcg cgggggtccc cggcctgatc tttcaatccc ggctggagga
    24841 ggcgctcgag gaactcgtcg acacgacgga gctggaggcg gcgacgctgc tcaacgcggc
    24901 gggcgaggtg gtcgccagcg cgggggggcg cgagctggcg ccgctggagc agcttccgca
    24961 acgcgccgtc cgctggcaga ccgactcggt cgtcgtcgtt aacctggtcg atttcggccg
    25021 ccggatggag gatgacggga cgacgctgcc ggcaacgatc gtgctgccgc gccgcgagga
    25081 ctggccgacg accggtttcc gcgagcgttt taacgacgat gcggcgacgc gcggcgaaat
    25141 cggccgcttc tcgcgccgac gctcccgcga tcgcgacgcc gacagcgagc gcatcggccc
    25201 gccgccgcgc ccccaggacg agggagagcc gggcgaccgc gcggggcccg gcgaccgcgg
    25261 acgcgaccgc gacttcttcc gccgtggctc gattcgcccc ccctggatga gcgaggaacg
    25321 ctaccgcgag ctgatcgcga cccgcgggct ccatggtttc atcctggagc tttcgaccga
    25381 tgcgctgcgc gagcagttgc gccgcgaccg ctggctgcgc gtgaccctgg ccctcggtgc
    25441 gctgttggcc gtcggcgggt tgggactggg ctggcgcgga ttcgaacgcg cgacgcggct
    25501 gcgcatgcgc ctgctgcgcg cccaggagat gaacgaacac ttgcgcgaaa tgaacctggc
    25561 ggcggcgggg ctggcgcacg agacgcgcaa cccgctcaac atcgtgcgcg gcctggcgca
    25621 gatgatcgag aagcgccccg acctgcccgc cgagacggcc ggccagttgc gccggatcgc
    25681 cgaggaggtc gatcagatca ccttccggct caatgagttc atcaagtttt cccggccgcc
    25741 cgagccgcga atcggcgccg tcgcgctgct ggacctctgc cgcaacgtga tcgagaacct
    25801 gcgccacgat ctggacgaga aacaggcgac gatcgatctg gcgggcgagc cggtccaggt
    25861 ggccgccgat gaggcgctgc tgcgccaggt cgtgttcaac ctgctgctca acgcggtgca
    25921 ggcgatcgac acgggcggac ggatccgcgt cggcatcgag ccggccgccc gcgatgccgt
    25981 cgtcctcacc gtcgccgaca gcggacccgg catccccccc gaactgcatg agtcggtctt
    26041 ccgcccgtac tacacgacgc gggccgacgg tgcgggcctg ggcctggcgg ttgtccggca
    26101 gatcgttctg gcccaccagt gggaggtcgc catcgccccc gccgacctgg gcggcgcggc
    26161 gttccggatc tcggggttgc gtctggtccg gcctccgtct cagaatgcgg catgacgcag
    26221 gacgccgccc ccgccgacac tccccccacg cttcccccgc tgttggtcgt ggatgacgat
    26281 ccgggccagc gcgatctgct gcaaagctac ctgacttcgt gcggatttcg cgtggcgacc
    26341 gccgccgacg gcgaacaggc gttgcgccat gtggcgcgcg agccggtcgc gatgatcgtc
    26401 agcgacgtgc gcatggcggg gatggacggg ctgacgatgc tgcaccggtt gcggcagtcg
    26461 cggccggacc tgcccgtcct gctggtaacg gcgtacgccg atatccgcga cgcggtcgaa
    26521 gcgatccgcg acggcgcggt caattacctg gaaaagccga tcgatctgga tgagctgctg
    26581 gcgtcggtgc gcggggcgct caagctgggc gaagcgaccc cgcgcgagga tccgctggcg
    26641 acgacggcgc tgggcgagga aatcgtcgcg gagagccgcc cgatgcgcga cgcgttgcgc
    26701 gaggcgctga tcgtcgcgcc gacgcgctcg accgtcctgc tgaccgggcg cagcggcgtc
    26761 ggcaagacgc tcgtggcgac ggtgatccac cggctgagcc cgcgggcccg ccagccgctg
    26821 acgctctaca actgcgcggc ggcgcccgcc gaggaacacg aggcggcgct gttcggcggc
    26881 gcgcagcccg accactcgct ctttcgcgag gcgcgcggcg gctcgctcct gatcgaggag
    26941 attcaggcgc tgtcgcccgt ggcgcagacg cgactgctcc actggctcga tccggcggcc
    27001 gcggggcccg accaggacga acgcccgcgc ctgatcgcga cggcgtcgcg cgatctggag
    27061 gcggcggtcc aggccggcgc gtttgccgag gccctgttct atcgcctcac gtcgttcgaa
    27121 atctacctgc cgccgctgtg ccagcggccg gacgacattc tgccgctggc ggcgctgttc
    27181 cagcgcggcg ccacccaccg gcgggcgcgc ttctcgcccg ccacgatgac ctgcctgcag
    27241 acctactcct ggccgggcaa cgtgcgcgag ctgaaaaacg cgatggaacg ggccgcgctg
    27301 ctctcaggcg gcgacgtgat cctgccggcg cacctgcccc cgcggattcg tgccgcggcg
    27361 gaacctgccg gcccgggcgc cgagaccgcc ggctcggcga cgatggaaga cgtcgagcgt
    27421 tcgatcatcc tgcagacgct gcgcgagcag aacttcaacc gctccgaaac ggcgcgccgg
    27481 ctcggcatca gccgccgcag tctgctctac aagctgcggc gctatcagga acagggattt
    27541 cagatcgatc cggactgacg gcgcggggcg gcgcggcgtc cggtcagtcg aaacgcagca
    27601 gccccgccgt ctcccaggcg cgcaccatgc cgtcggtgat cccggtgacc tgctccaggt
    27661 cgtcgggccc gcggaacggg ccgtaggttt cgcggaactg gatgatcaac cgggcgcggc
    27721 gcgggtcgat gcgcaggttc cgcgccagtt cctgctgcga ggcgacgttg acccgcaccg
    27781 gcgccggcgg gggggcatcg gccgtccgcg tggcggtcgt cgggcggtcc tcatcggtcg
    27841 tcgcggcttc ggccagcgtg cccaggtcga tcgccggcgg cgggagccgg cgcggcatcg
    27901 gcgtggcttt cagcatcgcc gccagctcgg ccggcgcgag accgaccggc gcttgcgcgg
    27961 tcgtcggctc atcggcggcc agcgtggcgg tggccgtcga ggggcggtcc tccgtgggct
    28021 gggcgctggg cgccggccgg cgcaggatca gccgcgccgg acccggcggc gccgccgttt
    28081 cggcggcggg agcggagctg gacggaactt gcgtgtcggc ggtgtccagg ccgctcagat
    28141 cgatgaccgg ttcggcggtc tggcggtcgg ggtagagtcg atcgagcagc gacggggagg
    28201 acgacccctt gtcggattga gcggccggca gagatggaaa gacgagagca agaacggcgg
    28261 cgaaagagaa aacggcaaga cgatcacgca cggagggggc atccttctga cctttggcgg
    28321 gacaacgcca tctgctgaga ttatcggcag ccgcgccggg aagggcaagc ggccggttga
    28381 gccttcgccc tcacgctgaa gcaatgatgt agactccatg ctctgtctgt ccctcgtgtc
    28441 ctttacgtcc cgtttgtccc ttccgtccct tccgaaatcc agatttccac ccaggtggcg
    28501 gttcggccga tccaattaag actcgctgga ccggaaaaga ggcttgcaac tgacaccgac
    28561 cctctgctaa aagtctagcc gacatcgaga gcgcgttgct cgcggatgcg gacgccaggg
    28621 gcgaggctgg attccaaccc cagcactcgt cggcgttcga ggaagatggt gagaatccat
    28681 cacgggcacg ccgctgtgac cggggacgac cgccgcccga tcagccactg attcttccac
    28741 cgaagaattg ggaaggcgcg gccagtagga cgatccggga gtcagaagac ccatccgcga
    28801 gtaccatcgc acctcttctt cgtgacttgg gaaggtggag cgtcaaacca gacggcctcg
    28861 ggcgaccggg gctgttcgcg gcccgcttcc ccggcgggct ttttcacatc cgccggggtc
    28921 ctcgccgccc atctcatccg gatcttcccc gcgccccgcg cggggcgggt gtctccgctg
    28981 gtctgatccc tcaccccaag cctccgccgg cggggcgggc atttgtccgc gccgtcggcc
    29041 caggccgcga atccccgcgc gctatctcga tcagaccagg tccgcgcggc tcccttggac
    29101 ccgcgggccg gcgctcgcgc ccgccgggcg cggcgctcgc cgacacctga ccggagccct
    29161 gaccatgtct cagcttctcc gcatccgatc cgtcacggcg ctgatcctct tcgccgtctg
    29221 cacgttcacc gcgcgcggcg agtggtcgcg tccgcacgcc ggcgcgggca acaacccgct
    29281 ccagccgatc cccgcccagc tgaccaacgt gaacgccgcc ccgctgtggg gggccgtttc
    29341 gctcaatctg ccgggcggcg cgacgccgac catcctcccc ccctacgtct tccacccggt
    29401 ccacgacgcg gcgacgacgc gcgtcaccgt cgtggcgctc aatgccgaga ccggcggcgt
    29461 cttctgggag agcgatccgc tggacgtcga caacacgatc aattacctct cggcgacgtc
    29521 gacggccgtc gacccggaaa cacgcgccct ctacttcgcc tccggcaata cggtctaccg
    29581 gctcgacgcc gacacgggga gcatcgtctg ggccacggaa ctgaccgcgg cgaacacgac
    29641 gccggggctc agcagctacg aaatcatcaa cagctcgccg caactgggca acgggatggt
    29701 cttcatcgaa acgttcggcg gcttcgtcat cgccaacaag cagcttgtcg cgctcgatca
    29761 aaccgacggc tcgatcgagt ggttcgtcaa cgacgtcggg ccggggacgt cgacgccggc
    29821 ctacgtcgag tcgggcgcgg ataagcgcgt ctattcggtg ggcgacctgg tggtgcgctg
    29881 ctacgacgcc gacgacggct cgctgatctg ggacagcctg agcgcgtcgc cgtcgtgggc
    29941 tacgtcgtat tcgatctcgt gcagcccggt ggttgaagcg ggccggctgt tcgtcggcag
    30001 cctcgatttc ttcaacgacg agacggcgct ctactgcgcc gacgcggcca ccggcgcgct
    30061 cctgtggcag gaaacggcgc cgacgaccga ttgccccccg ctggtgatcg gcgaccgcgt
    30121 cttcgtctac ggcggcctga ccaagggcac cctcgcgtcg ttcgcggtcg cggatgggac
    30181 tcccggcttc agcaaggaga ttttcaccgg ctacgtcttc cgcgattatc cggcggccgc
    30241 cgaggatgcc atctatctgt gcggcggggg cgacctggtg gtgctcgatc ccgccgacgg
    30301 gagcgagctg agccgcaagt ccgccggctt cttcggcccg gtctgcgtcg attcggcggg
    30361 cggcgtttat gcccacaata ccggcgcgct cgaggcctac gggattactg tgccggtcga
    30421 gctgagtgaa ttcacaatga attaatggaa gcagccaatg aaatttgaca tccgaaatcc
    30481 gcaatccgaa acaaactcca attctccaat tcggaatgag aacgggaatg gccgtcgggc
    30541 cccattggct gccgtcaacc gctatgcttt tcgaacattt cgtttattgt cattgtttcg
    30601 tgcttcgttc ttcggatttc gaatttttcg gaaaacggtc atcgcctccc gcctgaccgt
    30661 ggcgatgctg gggtgggcgc tgccgtgctg cctcgtggcg gcgcccatcc cggctccgtt
    30721 cagcgatccg gcgccgttca cgaacaacga cgcgccggcg ggtaaaatgc gaatgacgcg
    30781 ttccaccagc gggcaaatcg tctggggcga cgccccgcgc gtctcgctcg tctactggga
    30841 ggggcgcgag gacgccgtcc tgccacaccg ctcgtggttc cggcagtggg acccccatgc
    30901 cggctggggc gcgcgcgagc ggatcgagca atcgttcggc gaaggcggcg tggaggtcgg
    30961 cggccgccac ccggcgatgg tcgccctcga cgacgggtcg ttcgtcgccg tctggaatga
    31021 ttaccgccac gggctccatc cggcgtatat taccaatatt gagatttacg cgcagcgccg
    31081 cccggccgac ggctcgttcg caagctcggc cacgctcgaa cagcgcctct ccgacacgcc
    31141 cgacaacggc tacctgccgc ggatcacgcg cctgctcgac ggccggctgg cggtcgcctg
    31201 gtacgatttt cattataata acaatgaagc ctcggagctg gcgctcagga tcggcgcggc
    31261 cgacggcgat ttcacccagt cgcttgccgc cgcgccaatc cggctgacgg atctggccga
    31321 ccgcgccgcc ggcgacgagg gcaagagctt catcatgccc tcgccggccc ccgcgacgac
    31381 cgacacgctc gccctctgct gggtcacgct ggcggagctg tcgagcgggc tccccgacac
    31441 ctcctcgccc gccagtctgt atttcgggct gtatgacctg acggcgggcg catggagcga
    31501 gcggggacgg ctgctggcga accgggtgcg gggctatttc gatccgccca agttgatccg
    31561 ggaccccgcc tccggcgatc tgtggctgct ctacgtcgac agcgccagcc accccagcga
    31621 ggaggtttac ctcgcgcgcc ggcccgccgg cgccgggacg ttcgaagacc ccgtgcggat
    31681 caccgacgac ggcaaccgct cgcggtatcc cgatgccgcg atcgatgccg acggccgcct
    31741 gcatctggtc tgggtggacg atcccgccgg cagcggcaat tcgcacgtgc gctacgccgt
    31801 ttacgatccg gaattggccg cgctgacgcc gccggtcgat ctgacgccgg agcatccccg
    31861 cgaatgggag cgcccggcga tcgcgctcga ggcatcgggc cacgcattcg tcgtctggga
    31921 ggaaaatatc aacggcctgc agggccatct ctggtttacg acgaattgga ccccgaacgc
    31981 cgccgccgcg gcctggctga cggctgaatg aaaagctttc tgccacgacc cagcgcggct
    32041 gcgcccgcaa taaacgaacc cggaccgata ctcgcaaagc ttccaaggca cataatcgat
    32101 ccgatagccc attcgtaatc ggtgttggta tcgggagcgc tatcgctatc gagttcgatc
    32161 gcgcgcccgg ctttccatcg cttccattat ctcccttccg ctccccccgt cccttccaga
    32221 ccctcgatcg tgatccggcc ggcgatgatg tccgcgatga cgtcgctgta aagcgtgtgc
    32281 tcgaccgcca gaacgcgctg ctggagcgtc tcgggcgtgt cgcccggcag gacgggcacg
    32341 cgcttctggc gcaggaccgg ccccgtgtcg tattcggcgg tgacaagatg cacggtcgcg
    32401 ccgctctcgc gcgcgccggc ctggagcacg gcgatgtgcg gcgcgatgcc gtacatcccg
    32461 gcgccgccga agtcgggcag cagcgccgga tggatattca ggatccggcc ggaaaacgcc
    32521 gccagcgtct tcggcccgat cggtcgcatg tagccggcca gaatgaccag ttgcgcgccg
    32581 gcttcccgca gcgcgtcgcg gatggcggcg tcgagggcgt ccctgtccgg atgcgtgcgg
    32641 ctgctcaggt ggcgccatgg cagaccgtgc cggcgggcgc gttcgagcgc ccccgagccg
    32701 gagttgttcg agatcaccat cacgatgcac gcctcgagtt ccccggcttc gatccggtcg
    32761 atgatcgcct gcatgttgga gccgccgtgg gaggcaagca cgccgatgtt caacatgatc
    32821 gcgcctcagt tctcggaatt gtcattgcca ttaacattgt cattttcctc gccgccgccg
    32881 ccgccgtcgc ctccgcagcg ccacagcgcc cagcccgact cctcatcctc catcagcggc
    32941 gggccgtccg caagcgcttg gggacggcgc cgggccagaa tgtagccgat gccgtgggcg
    33001 cggatctgct ccggcgtgac gcgaccctct tccgtcagcc actcgtcgag gatcagccgc
    33061 tcggtcacga tgcagacgcg gaacgtctgc agcgcgggga cctgccgcca gtcggcgtgg
    33121 tcgcgcacga ggatcggtcc ggccgtgcgg gcgtggatcc cgcgcgccag atccctcaac
    33181 cccggcggga tgtagggcca ttggcgccac gcggcgccga cgtgattcag catgccgagc
    33241 acgagcgccg tccccacggc cagccgggcc gttcgccgcg ggagccggcg cgcgagccgt
    33301 tcggcggcga gggccgccgg cgccgccgtc agcagcgcaa catcccagaa gaagcggatc
    33361 gacagcggga acaagctctg gcggtggacg gcccacgccg cgctgaccag cagcagcagc
    33421 gcgtaacagc ggacggcggg gcgccggcgc ccccggacca gttccgcgcc gcacagcacg
    33481 gcggcgaggg gcatccagac ggcgttgatc tggaggaacg tcaacgcgat cgctgcgttc
    33541 ggatgaaccg gcgggatcga acctgcgtag tcgccggcgt gacgcagaaa ttggaagggg
    33601 tcccccagcc gcagccatga gacgccaagc cacaccgcga tcgccgcccc cggcggaatc
    33661 aggatcagcg cgccggccca gcggtcgagc ggccggctcg tgcgcgcggt cggcagcacg
    33721 acggcgcaac cgagcgcggc gccgctcagc cagggttcgt agcgcgtcag gcacgccgcg
    33781 gccagcgcgg cgccggccag cgcgagccgg cgccgggtga agccgcccgc cgtcagcgcg
    33841 cggaggacca ccgccagtgc cgccgccatc cacaagcctg cgtcgggctc gctgtagttg
    33901 gaggcggcca gatgaaaggc gccgaggttg aacacgtaaa acgtgctgaa aatccacacc
    33961 gccaggcggc cgccgcccag cgtgagcatc acgtccgcca gcaggcgcca cgtcccccac
    34021 aggccgccga gcgtcaccag ccgcatgagg aacaacgtca gcgcgggata ttgcagcgcc
    34081 cacgggctga acagccgcag ccatgcgccg cgcatcgccg ccggcagcgg cagccagtag
    34141 ccgtcggtca tgaagaacgg gcggacggcc caccgccgcg cggtcagcgt ccgcatcagg
    34201 tcgtccaggt cgcggaaatc caccccagcc caaacttcgt aaagctgcgt cagcagcaac
    34261 agggccagga cggccgcacc ggcgaggcgc aaatcacgcg cgaacgacgc ggcgtgcgcc
    34321 ggcgcggcgg gcgggggcgc cgccggcggt tcggcctcca gggcgcggcc gcgcgtcatc
    34381 ggcgcttgcg tccgttgcgt cgcgcccgcg ccagcagcag tttgcggggg tcctgcccgt
    34441 cgccggagga gtcggccgcg gccgcggcgg cgcgccgggc cgcctggtcg accttgtcgc
    34501 ggaacatcag cgaatgggca tggcacaacg cggccgccag cgcgtcggcg gcatggtcgg
    34561 gccgcgggat cgtctccagc ccgagcacgg cgcggaccat catctgcacc tgcgccttgc
    34621 cggcccggcc gtgaccggtc agcgcctgct tgatctgcag cggcgtgtat tcaaacaact
    34681 cgatgcggcg gcgcgccgtc gccaggatgc acgccgcccg cccgtgggcg accaccatcg
    34741 ccgtcttcac attgcgggcg aagtaaagct gctcgacggc gacggcgtcg ggccggtgcg
    34801 tttcgatcag ctcggccagc gactgataaa tcgtgtccag ccgctgggcc agtggggtcc
    34861 cgggcggggt ttgaatcacg ccgtaggcga cgagcgaacc gcgccggcgg gtcccctgcc
    34921 agtcgatcag gccgtagccg acgtcggcca gccccggatc gacccccagc acgcgcaggg
    34981 tcggtggctc tggtcggggg tgcggcatgg aaacgcaaac gcccggcgcg cgccgggtag
    35041 ggtgtcccgc gacggggtcg aagcgcccgg acccggctgg gccgcgcgcc tactgcgcgt
    35101 attgctccag caccgcgtcg tcgatttcct cgttggccga aacgctcgtc acgtcgtcgt
    35161 gctcctcgag cgcttcgagc aggcgcaaca gcttgggggc atcctccccc tccaccttga
    35221 ccgtcgtgct ggggatgttg cgcagcgacg acgactcgac cttgatcccc ttggtctcga
    35281 tcgccgcgcg cacgtcctcc atctggttcg gctcggtcag gatccggtag tattctcctt
    35341 cattctccag gtcctcggcc cccgcctcca gtgccgcctc catcaccgtc tcctcgctct
    35401 ggccctgggt cgggatcagg atcacgccgc gcttggtgaa catccaggcg acgcacccct
    35461 gcgcgcccag gctgccgccc cccttgttga agatcgcgcg gatctcgggg atcgtccggt
    35521 tcttgttgtc ggtcgtgcct tccaccagga tcgcgacgcc ggcctggccg tagccttcca
    35581 gggtgaaatc ctcgtaggag accccttcga gctccccggc ccccttcttg atcgcccgtt
    35641 cgatgttttc cttgggcata ttggcgtcgc gggcggcggt gacggccgtg cgcagccggg
    35701 ggttgccgtc gatgtcgctc ccccccatcc gcgccgccac ggcgatttcc ttgatcaggc
    35761 gcgtgaacat cgcgccccgc ttggcatcca ccaccgcctt tttgtggcgg atcgaatgcc
    35821 atttagaatg gccggacata cctggtctac cttcaacttg ttgagtgtca cgcgcgggcg
    35881 cggaccgctg gcccgtcccc cgccggctct tgagcgtgcg caaggcgcct tgtcgctatt
    35941 agacagaatc aacgggcttc aatgcaagcg cggttgaaac ggacgccggg cgacggggat
    36001 cgcctctccc catcgcagcc ctgtcagtat cgggatcggt atcgcccgcg cccccactcc
    36061 tccgccatcc agcgacccat ccatcggcgg acgacttcag aaccgggttt cagtgccggg
    36121 ttgcgtcggg ctggcttcgt tgcggccgtc gagcggcggg ccgccgggcg tcccggcgcg
    36181 gcggcgctcg cgatcacgcc ggcccggccg atcggcctgg cgccgttcgc gccgtgcctg
    36241 gtcgccggcg gcgacggcgt cggtcggtcc cgccggaccg gccgcctcgg cgcgggagcg
    36301 gccggccgct gcgccggaga ccacgccgcg ctcggtcccg ccgaaactgc ccgccgggcc
    36361 gcgcgcggca gggggcgcct gcggccccgc gccgccgacg accagccccg tctcgaccgc
    36421 gtcgggatcg acatacggct cgtcgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36541 nnnnnnccgc cgacgaccag ccccgtctcg accgcgtcgg gatcgacata cggctcgtcg
    36601 cgcagcagcg cggcgcccaa ctcctcgccg ccggtttcaa aatccgggat cgcgatcggc
    36661 tgggtctcga tcggcatcgt cgtcggcgcg tcctccagcg ggatcgccgt cggccggatc
    36721 tggcgcaggt attcctgaat ttccggcgga acctcgttca ggtcctcggg cggcgcaacg
    36781 gcctggacca tcgattcggc catcgcctgg cgcgccagtt cgttgaccgg ttcgacgatc
    36841 aggtcgttct tcggccgttc ctcgatgatg tgcggcgtga tgaaaaacat caggttgcgc
    36901 agcgtgtcgc gcttggtctt gttgccgaac agcgccccca ggtaggggat ctgcgacagc
    36961 acggggatgc cgctgctccc ttcgttgcgg tcgcggctga tcagcccgcc gatcacccgc
    37021 gtttcgccgt cggggatgat caggatcgtc tcggtgcttt cctcggcggt gccgacgcct
    37081 cgcgtcccgt cgcccagatc ctcgacgatg atcggcgtcg aattctcgaa attgatctcc
    37141 agctcaacca gcccgcggtt gctgatgtgc ggcgtgatgt cgacgatcag tccgctggga
    37201 atcgtcgtct gactgcgcgt gcggaaatcg ctgttgatgt tgttggaaaa gaaggtctgc
    37261 aggaccggct cgttgcgcgt cacctcgaac gtccccagct cgtggttggc gatgatcagc
    37321 cgcggccgca gcaggatttt cgagcgtgaa tcggtcagcg ccgcgttgag ctgagcccgc
    37381 acatggtcgg tcagttccag caccgacagg ccgctgctcc cgctgctgac acgcgggaaa
    37441 gtttcctcat cgaggttgac ctcgtcatcc tcgtcgacgc ccagcacccg gatcagctcg
    37501 ggcgagtact cccactccaa cccgtagcgc aggtcgttgc tcatcccgac cgagagaact
    37561 tcgccctcga tcagcacctg tttgcgcggc cggtccagtt gcagcaggat gtcgtacacc
    37621 ttgtcatgga cggccttgac gtccttcagg atcagccggc cggtggagac ctgaaaccgc
    37681 agcagggcgt cttcgctcac gatcggttcg atgaaatcgc tgacgatctc gtcggcgatc
    37741 tcgccgtcca gaccgatgtt cgtcagatta tagatgaaga tctcctggtc gcggtcgagc
    37801 agttcgacca gttgttccat gatcttgatc ttctcgaacg tgtcctcgac gatgatcagg
    37861 tggttgaccg ggtcgacatg gatgatcgcg gaggtcgact tgatttcggc gaggcggtcg
    37921 gcgacctcct cggtgctggc gttgcggatc tcgaagacgc gggtgaaaag ctgcgtgtcg
    37981 tattcgttga tgatgctctc gatgaacgtc atccggtcgg gcatctcggc gacgatcagc
    38041 ttgttggagc gctcgtcggc ggcgatcgag ccgatctcgc gattgatcat cggttcgagc
    38101 accgtcgcca gctcgcgcgc gttgatgtat ttcaaatcga agacgcggcg gatgacctgc
    38161 ccggcctgca cctcctggac gtaggtctgc tggtccatga tctggtagct gccgtcgggg
    38221 aacttgaccc agcgccagcc gcgttcctgc gcgatcgatt cgaggatctg ctcgaccggc
    38281 gtgtcgcgca ccgccagcga gagggtctgg ccctgggcct gcccgcgagc ctcgatccgc
    38341 acccccgttt tttcgcggat ttcgttgaac acgtcgttga ttggcttgtc gcggacgacg
    38401 agcgtcgtcc gcgggctggc cggcgccgtc accgcgatcg cttcgccttc atcgagcgcc
    38461 accccgccgc tctcgaggac attgacgggc gcgcccggcg tcgagccggc ttcatacaga
    38521 acctcggggg cctcgtccgg gccaatccgg atctccggcg gggcgggcgt ggccgtcggc
    38581 gccggcgccg gttccggctc cgcttcccct tcggccggct gcgccggcgc cacgccggcc
    38641 agcagcagcg ccagccccca gccgaggaat ccggcggcgg cgcgcgccaa cccgcgcccg
    38701 gcgcggcggc gccgcgcacc ggggacgatg cgcgatgtac gatccagttg aagcggcaaa
    38761 ggcggcgttc ccttccgtgg gcggacaggc tctggcatca gtaggccacc cagttttcct
    38821 gaccttcata gccgagcagg accttaaatt catccagatt tatcgtcagc agcttgacgt
    38881 cgagctcctg gccgtccttg tcggtggcgc ggcgcgtctc ccccacgcgc atcgtgaaga
    38941 cctcgcccga ctccggccga ttgtcgatga actcgacctg attctcgtcc atgctcgaga
    39001 tctgccagga aaacaccgct tcgagcaggt tgggcggctc cggcgcgggc gtcggcgtcg
    39061 gtgtcggggt gggcgtcggc gtgaacagtg cgccgaacat cggcgaacgg atcagcggat
    39121 cgtacggcgt ttccggcgtc gacgggatgc tcgccggact ttcaaagtcg gtttcatccc
    39181 cctcgttcag gtaggccgag atgtcgcgca gctcgaccgg cttgctgtac tcgaccgtcg
    39241 ccaggtagaa aatgaagccg ccgctggcgg ccacgagcag cagattgatc accatcagga
    39301 tgcgctggtt catgggcggc gggtttccat gcagatccga aacgccggct cctggcggta
    39361 gagggcgagg cgcgcggcgc tgtgttgcct cagatacggc gcttcatcga gaaaatcctg
    39421 gatcagcgcc tcgtctttgc cggcggcggg gcgcagcgcg cggccgatcc gctggtaggc
    39481 gcgcgtcggg ctcttgcccc cgcccgccag aatcacggcg tccagcgacg ggacgtccaa
    39541 cccctcgtcg gccagcgtcg tggcgatcag cagggggtgc aatttccgtt ccagttgctg
    39601 gagcagacgc tgccgggcct ccccttccag caatccgtgg atgaattgag cctccggcag
    39661 caactggcgc aggagttcac catgcgcgat ctgaccgacc agcaccagca ccgaccgccc
    39721 gtcggccacc cactggcggg cccgagccgc gatcacgcga ttgcgttgct ggttttccac
    39781 gatggcccgc cgataccact cctggtactc gacgcgtgcg ccgggggccg cgcgcacccc
    39841 cccgaccggc gtcatgtgga tccgcggcgc gacgagatac ccctgctcga tcagctccga
    39901 gcagttgacg acggcgagcc gctcgcccag cgcggcctcc agcagcatgt cgctgtcgtc
    39961 gtcgcgccag ggggtggccg aaaggccgaa gcgccacgcg gcgcgcccgg tgcgcatcgc
    40021 cagcgtgtaa aacgtcgccg ccggaacgtg gtggcactcg tcgaaaatgg cgacttcggc
    40081 gcgttcgagg gcgtcgcgga tctgctggcg cgtcgcctcg tcgacctgca gctcccgttc
    40141 ctcggggcgg gcctcttcgt cgtcgggcgc cgcgcggcgc cggcgcggcg ccgcccgccc
    40201 ctcgaaggcg cgcgtcgccg tctggatcgt cgccaccgtc accgggcgca ggttggcctg
    40261 gccgtcgccc aggatgccga tctcaacgcc cagttcgcgc tccagcacgt ggcgcgtctg
    40321 gtgcagcagg tcgagcgtgt ggacgaagaa gaaggcgggc cgcccgatgc ggcggatgag
    40381 gtgcgcggcc agcagcgtct tgccgccgcc ggtggcgatc tggatgatcc cgcgcccccc
    40441 cgccagcgcg cgttcgagcg cccgctgctg gtattcgcgc agcggcgtcg gggcgccggg
    40501 gcaggcgagg ggggcgcccg gttcggcgcg cggcgcgccc gccagggtat attggacacc
    40561 gtgggcctcc aggcagccgc ggacgcggga ccacagcccc gtgggaaatg tcccgcggcg
    40621 gatgtcgaac aggtgcttgc ggccgtccca gcggccctgt ttgaatcggg gggaatactg
    40681 gtggccggcg atgggaaagg aagtggcctc atcgatcgcg cgcagcggca ggccgctgcc
    40741 ggcgatgcgc ccgtggacgg gcccgagctc gaccgcggcg ctcacccgct gccagtcccg
    40801 ccggaagcgg ccgcgaatct cctcgctgac gtcccccggc ttcagcccga gcagcgggag
    40861 caactcggcc aggtgcgaca gatgaacctc ccagcggctg tcgcggctgt tccagcgccg
    40921 gttctgcagg cgcttgatcg aggcgacggt gccgcgctcg tagggaaacg acacgccgag
    40981 cgtctcgtcg tcgagcagtt cgattttgac cggcatgccg tcgctgtcac cccgccgcgg
    41041 attgcgccac gccccccgcc ggtgttatgc ccgaattggc ggggggcttc aagcccgccg
    41101 tctcgcgagc ggcggagcgg cggggtgcct gcgatacgat gtgaaagcga ggcgttcgat
    41161 ggacaacggg caagcagggc cgcgcgaaga ccggctgccg atccacgcgg tgctggacaa
    41221 cctgcgcagc gcgttcaacg tcggatcgat cttccgcacg tcggacgccg gccggatcgc
    41281 cgcgctccac ctctgcggga tgacgccggt cccgccgcat ccccggctgc tgcgcacctc
    41341 gctcggcgcg gagcagcatg tcccctggag ccacgagccc gatcccgtgg cggcctgccg
    41401 cgcgttgcgg gcgcggggga tcatgctggt ggcgattgag acgcagcccg gcgcggcggc
    41461 gcacacgcgg ttcgactggc cgcggccggt ggcgctggtc ttcggccacg aggtgcgcgg
    41521 catccggccc gacgtactcg gctgctgcga cgccgccgtg cggatcccga tgctgggcca
    41581 caagaacacg ctcaacgtcg cgacggccta cggcgtggtg ctctacgaga tcctgcgcca
    41641 atggcgcatc gtctgacccg ccggcgcgcc gcgccgcggc cgcaaaagag cgttgcataa
    41701 cggcggcggg gacgcacaat gagcgcgagt cacaccggcg gacagcttcc gtggagaccg
    41761 ggtcgtgaga aaacggcgca tcatcctcgc gtatcacaac ccggtgatca aattctattg
    41821 cctggtccgg ttcatcatta tccggatcaa tatcctggag aaattctccg agccgctgcg
    41881 ccagttcacc agcgggcatg tgctcgacgt cggctgcgga ttcggcctgt tcgcgatcta
    41941 caatgcgacg cggcaccccg gcctgacgat ccatggcgtc gactacaacc gccgccggat
    42001 cgccgaggcg ctcgccgtca gccgccgcct gcgcgtggac aatgtccggt tccgcgtcgg
    42061 cgacgtgcgc cggcgcgacc cggaggtccg ccatttcaag gtgatttaca tgctcgacat
    42121 cctgcaccac atccccgtcg tctccaaata ccggctgctg cgcaaatgcc accggctgct
    42181 ggaggacgac ggtctgctgg tgatcaagga catcgacacc ggcaaccggc tgaaggtggc
    42241 gttcacgtgg ctgctggaca agctgatggg cccggtcgac gccgtgagct accagtcgtc
    42301 gctcaagctg cagcggatgc tggagtattg cggattcgag gtgacgcggc gcgagttgat
    42361 cgatgatatt ctgccctacc cgcacatggt gctgctgtgc cggaagcgag cctgaagatc
    42421 gtattagccc gcgggccgtg ttttcgcttg aatgccgcgg cggcgggggg tgtgatgcgg
    42481 tagagcggaa tcacgccggg gaaaggagcc gggactcatg ggcgctgcga gagctccgat
    42541 cgtggtggtg ctggccgacg cggccgagcg cgatgcggtc cggtacgggc tggaccgcct
    42601 gcgcgagctg gaatgcggct tcgacgtggc gctcgttccg gccgccgccg atccgcggct
    42661 ggccatggac ctggcgcgcc aggccgagga ggaaggccgc tgcgtcgtcc tggccggcgg
    42721 ggagcgcctg ggctggctgc ccgacgtgct cgccgccgcg tgccggctgc cggtcgtcgc
    42781 catcggtccg ccgggagctg cccccgccgg cagtcaggcg gcgtgggtgc gcgaaccggt
    42841 gccggccgcg ctgatggcgg cgcgcatcgc cgccgtcgcg gaccccgcgc tggcgcgccg
    42901 gctgggggag gacgcggccg ccggtccccg cccgtccgcg ccgccgctcg agccggccgc
    42961 gcccgcgtcg ccgcgcgccg agccgtggga cgaggacgct tcggccgaag acgaggacga
    43021 ggcctggaag cggggctggc gcacggcgcg gccgcatccg gaggagccgg ctgaagaggg
    43081 cgaagagcgc gttgaaatcc ggccggtcga ttggaaccgg ccgttaaagg ggtcggtggt
    43141 gggcggggcg caccgccctt cgcgggaaga ggaagcgccg ccggcgccgc cccccccgcg
    43201 ccggctgggg cggatcgcga tcgagccgga gtcgcccgag ctcgacgtga ttgaggaagt
    43261 cgtcgattgt ctcctcgagg ggggtgtcgt ggcgctcccc accgacacgg tctatggcct
    43321 ggcggtggat gccaccaacc ccgcggcggt ggagcgcctg ttcgaactca agggccgcgg
    43381 ccgcgaaaaa ccgatcgtcc tgatggtgga ctcccccaag ttgctcggtc aggtcgcccg
    43441 caacctgacg gtggcggtgc gccggctgat ggaggccttc tggcccggtc cgttgacgct
    43501 gattctgcag aagcgcgagg ggaacttcac gcatatcagt ccggggccga cgatcggcgt
    43561 gcggctgccg gaccactcca cgccgctgac gatcatccag gcgctggcgc ggccgatcgc
    43621 ctgcacgtcg gccaacccct cgggcgcggc cgaggcgcgc tccgccgatg aagtggagga
    43681 ctatttcggc gacgaactga gtgccatcct ggacgccggt ccgctggctg agtcgctccc
    43741 ctccacggtg ctggacgtga cggcggaacc gtttcagatt cggcggctgg gggcgttggg
    43801 ccgggagcag ttggccgccg tcgtgggcga tgtcctggag ggggattgag gcggggcgaa
    43861 cggcggcgaa cctcggttgg cgccgcgaac aatgcttgcg tcttggaggg gtttaagtgg
    43921 actatttgcg ggcaacttag cgtcgttcgt gggccggccg gccggcattc ccctgaggaa
    43981 tccgcttacg gtcggtcgac agccttgcgg cgcttggcaa ttctccacag aaggagcgcg
    44041 gactatgcgc ccattgtgcg tcctgacgct ggccctggcg gccggcctcg ccctgaccgg
    44101 ctgcgtctcg tggcccggcg gcaccgacct ggccgcggtc gccagccgaa gcgggatgag
    44161 caacgtcacc acgggggacg gcacgttcga gaattacacg gccaccggtc attacaccgg
    44221 catggacgtg ggcatcgccg tggggattcc gttcctgacc aagctgtttg aaattttccc
    44281 catgcgcagc aacgaggaac tcctcggcga cgtggctcag accgcggccg gcgacggcgc
    44341 cgacgcgatg atcaacgtga cgccggccgc cagtgtcttc accggcttcc cgttcggcat
    44401 cctcgggatt tacgtcgacc gcgccgaggg cacgggcatc cgctcgcgct gatctccggc
    44461 acggctggcg ctgctgtcac tggaccgccc cattgcgtcg gggcggtctt ttctttgacg
    44521 aattcttgga caagccgccg ggggatcgcg ctaagctgcg gtcggtgacg tgccatgcgg
    44581 attgacgtga tcaccctctt tcccgagttc ttcggcgagc cgttgcggac gagcgtcgtc
    44641 ggccgggcgg tcgcggccgg acgcctcgcc gttcacctgc acaacccgcg cgactggacg
    44701 accgacaagc accgctgcac cgacgacgcg ccctacggcg gcgggggcgg gatggtgatg
    44761 ctggccgaac cgctgctgcg cgcgatccgc ggggtgcgcg gcgccgccga agcggagtcg
    44821 gttctccgcc cgccggtcat ccacctgtcg ccccagggcg aacgcctgac gcagccgctg
    44881 gtccgcgagc tggcgtcgct cgcggggatc atcctgctgt gcggcaacta cgaggggatc
    44941 gatgagcggg tgatcgagct ggaagtcgac cgggagattt cgattggcga ctacgtcctg
    45001 acgggcggcg agttgcccgc gctggtggtc atcaacgcga tggcccgcca cgtggagggg
    45061 gtgctgggca atccgagctc ggccgagcgc gattcgttcg aggatgggct gctcgatttc
    45121 ccgcactaca cgcggcccga aacggtggag gggctggcgg tcccgccggt cctgctgtcg
    45181 gggcatcatg cgcggatcga ggcgtggcgg cggcaacggc aactggagcg caccgcccgg
    45241 cggcggcccg acctgctcgc gcgcgcggcg ctgaccgacg aggaacggcg ctggctggcc
    45301 caacagggac tctggccgcc gaagggggac gccccatgct gatggttgcg ctggtgcact
    45361 acccgacgat caacaaggaa gggcgggtgg tgacgacctc ggtgacgaat ttcgacatcc
    45421 acgacatcgc gcgcgcgtcg acgacattcg gcgtcgcccg ctactacctg gtaacgccgg
    45481 tggaattgca gcagcagttc gtccggcgga tcgtgcacca ttggctggag ggctcggggg
    45541 cggagtataa cccgacgcgc aaggaagcgc tgacggccgt ggagatcgcg ccggacctgg
    45601 cggccgtggg cgaggcgatt gagcgcgact tcggccgcgc cgcgacctgg gtggcgactt
    45661 ccgcccggcg ctacggccac acgatcagtt gcgcggcgct gcgccgccgc ctgcgcgacg
    45721 agccggacca accggtctgc ctgatcttcg gcaccggttc gggcctgcat ccgcagatcc
    45781 tcgaggaagc tgatctgatc ctggagccga tccaggggcc gacggcgtac aaccacctgt
    45841 cggtccgctc ggcggtctcg atttatctgg accggctgct cgccgtgcgg ggggattgag
    45901 cctcgcgcgg cgcccgcggc gcggcgacgc gatgatcccc gttttgggaa ttcattggca
    45961 tgtcatgcgc ttgttggagg atgatgcggc ttcggattcc acatgaaccc gcgacaatcc
    46021 acgaaagcga tcactcccat gaacagcctg atgaagacgg cggcgctggc gctcggcctg
    46081 gcgctctgga gcccggtgtt gatggcgcag ccggcgcagg aagggcccca gccgcccgcg
    46141 gcggccgcgc cggccgaggc gtccaaggtg gtgttgaagg tgaacggtca ggagatcacg
    46201 gcggcgcagt tcaatcgagt cctccagcag atgcgcgcgc agcccagccc gccgatggcg
    46261 cagcaggagc cgccgacgat ggaggaggcg caggccgagc tggagcgtca ggcgcgcgag
    46321 cgcctgatcg gccaggccct gttcacgcag gcgctggagc agaagtcgcc gacgattccg
    46381 gatgcggaag tggatgcgcg gctgagccag ttcgagcagg aagtggcggc gtcgggcatg
    46441 cagatcgatg acatgctgga gcaggccggg aagacccgcg aggatctgcg ccaggagctc
    46501 cgccaggacc tggcggtgca ggcgctggtc gaggagcata tgggcgatca ggatcccagc
    46561 gaggaggagt tgcgccagtt gttcgaccag gtggcggacc aattgaaccg gcccgaacag
    46621 atccagacgc gccagatctt cgtggccttc acggatgacg gcacgacgcc gaccgaggaa
    46681 cgcaagcaga cggcccgcca gcgggcggat gaggcgttca aacggctgaa ggcgggggag
    46741 gacttcgtta aggtcttcga cgagctgtcg acgccgatgc cggcggcggg cgaaggcgga
    46801 gaattcccgt tccacgcgcg cggcgagttg ccgccgccgc tggacgacgc cgcgtgggcg
    46861 ctcaagccgg gcgaactgtc cgaggtgctg gagtcggaat acggcttcca catcatccag
    46921 ctcgaggagc accgcgaggc ggcgcccgcc tcgttcgagg aacatcgcga tgcgctggcg
    46981 cgacatcaac tgcagcagaa catcatgatc aacgtcccct tgacggcgct tgagctgcgc
    47041 aagacggcca aaatcgagat gctggacgag acgacgggcg agttcgtgcc ggcgccggcc
    47101 gccgcggagc cgaccgcgga aggtgagccg gtcaacgaaa actgaccccg aagacctgac
    47161 gcggcggggc gcaaggcaga acaggcaggt tcagaatcgg gatcggtatc gatatcgcta
    47221 tccgtctcgg gagccatcgc gaggacgagg acgagggacg tgctctcgat cgagggagtg
    47281 tgaccggggg aggacgggcc gatggcttgg ccggaacgct ggtttccatt cgtgttgctg
    47341 gcggtattgg gagcggcgag cgttgaggcg ggtgaggatg ccgggacatc cgcgacgctt
    47401 ctgccggccg cgccgcccgc cgtggaactg gacgtgaaat acaatatttc gccggaggag
    47461 ctgaaccaga tcctggcggc gattccggcg gcgccgcggg ccgaaccgcg gcggccgcgg
    47521 cgcttgctcg tgttttcgcg cgcggccggc ttttaccatg acgcaatccc tcacggggcg
    47581 cagctcttcg cccatctggg cgcgcgcacc ggcgcgttcg cggcggtcgt gaccgatgat
    47641 ccggcggtgt tcgagccgga ttccctgagc cgattcgacg ccgtgctgat gaacaacacg
    47701 accggcgaga ttttccggcc ggggaactat gcggagctgg acgatgccgc gcgcgccgca
    47761 gccgaccgga ccgcggcgcg ctatcgcacc agcctggcac agttcgtggc gcgggggggc
    47821 ggtctggctg gcattcacgc cgccaccgac acgctctacg aatggccgga atacggcgag
    47881 atgatcggcg gttacttcgc cggccatccg tggaacgagg aggtcggggt cacggtggtc
    47941 gagccggacc atccgctgac ggcggcgttc gcgggggact tcacgatcgc cgacgagatc
    48001 tatcagttcc gcgagcccta ctcgcgcggg aaggtgcgcg tgctcctcgc gctggacccg
    48061 gcgcggaccg acatgacccg gcccggcatc aaccgggacg atcttgattt tcccatcagt
    48121 tggattcgga attacggcgc gggtcgtgta ttctatggct cgttgggtca tcgccgggta
    48181 attttctggc atccggcggt cgttcagttt tatctggatg gcatccaatt tgtgttgggt
    48241 gaccttgacg ccgacgcgga ccccgtgccg ggccagtccg agccctcgga gcacccctga
    48301 gcgacaaccc ggatgaacct ctcgatgcga ctcccgttct tcccgctctt cctgttcgtg
    48361 gtcccgctgg ttctgctcag ttgcgcccgt ctggaggaac cgacccatta tccgacgctg
    48421 tcggagccgg tgcgcgcggt gctgttgcag ttgcccgagg ccgatgtcca gcagcgggtg
    48481 gacctgcgca tcgcgccgga gttggagcag cagctggccg cgctgcggac cgaacagatc
    48541 cgcgaactga tcgactacat cgtcattggg gatgtgaacc aggacctccg gccgcggtcg
    48601 gcgatccatg cgctggtgct gctggcgcgg ccgccggcgc cggcgctccg tcgggcacaa
    48661 ctggccggcg ccatcggcga cgcggtggcc gatggccgcg tgtcccgcgt gacgcgcgaa
    48721 ttcctggtcg gccagttgcg tttcctcggg ggcagggaag cggtcgatgt gctcgcccgc
    48781 gccctgcagg acggggagct ggcggaaccg gccatcgtcg ccctgctgga gatggaggag
    48841 gggctcatcg gcgagcatga gcggctcgag acgctccaga cggcgtatcg gcggagccat
    48901 gggtcgcggc gcgcgacgct gttgcgggcg ctgggacggc tgggcgatcg gtcgctgctg
    48961 cgcgaatttc gccaggatgc ggagtcaacg aacattgaaa tccgggaagc ggcgctcgat
    49021 gcgatcgccg cggcgggcga cccggccgat gggtcgcggc tgctgcacgc gctggcgcgc
    49081 gccacgcccg ccacgcgcct gcaggccgag aaccgctacg tgattttcat cgatcgcatc
    49141 ggccgggacg atccggaggc ggggatcaac ctgtcgcgcg agttcttccg ccgggcgtat
    49201 ctggaccagc gcgggcaggc ggccgccggc gcgctgcgcg tgctggccga tctgcagggc
    49261 cccgacgcgc tggaccagat cggcgcggcg ctccagagcc acgatccgga tctgcgcgcc
    49321 gcggcgcttg aaatcgccgc gcggatgccc ggccaggatg tgacgcgcta ctgggtcgag
    49381 atcgtgaaca acctggagga ggcggagttg cgcgccacga tcctgcacct gttggcggca
    49441 cggggcgacc ggctggcgct ggcgtgcgtc gagcgctccc agcgcgaccc cgacccggcg
    49501 gtgcgccagg ccgcggccca tgcggcgtcg cgactgcacg gcgagacggc catcgccctg
    49561 gtcgatcagg ccggcgcgac cgagattccg atcacgcagc cggatctgaa acagacgctg
    49621 agcgcgcggc tccagacgga gcccgacccc gagcggcgaa ccgtggtgct gttggacgaa
    49681 ctggcccgcg ccacgcagcc aggggaaaag gggacgatcc tgggcctgct ggcgcgggag
    49741 ggaggcgacg ccgcgctgtc ggcggtgcgg ggcgcgctga tcgatccgga cccggcggtg
    49801 cgcgaggcgg ccacccgggc gctggcggag tggcccgatg cccggccgac gcccgatctg
    49861 ctggcggttg cgcgcggcga ggacgaggag cggctgcgcc tgatcgcgct gcgcggggcg
    49921 gtgcggctcg tcgaccggga tgcccggctg cccgcggcga gcaaactggc gtggtacgac
    49981 gaagggatgc ggatcgcgtg gcgtgccgag gagcgccacc agattttggc gagcatgcag
    50041 caggtgaagg acctggcggc gctgcgacgg acggcgcagt ggctggacga tccggcgacg
    50101 gcgacgcagg cggccaccgc ggcgctgcgc atcgcggcgg agctttcgcc gagcgagcgg
    50161 gccgaggcgc gccagaccct ggagcggatc gcccggatgg aggatcccga gctccggcgc
    50221 gaggcgctcc ggcttcaggg ccgctagcgt cgggacggct ggctcatcgc ctctatccgt
    50281 ctcttccgtc cttgatgtcc cttctcgaac attcattctc gtacgaagcg aggaccagag
    50341 tccaatcggg agcgggaggc aaagtagccg ggggccggtc ggggaggcgg attcgttatt
    50401 gcgcaacggg ggaaatgggg gttcaataaa cggcttgcgg ccgcggcgcc ggcggtcaat
    50461 ggggattgac acgtaccggc ctctgtggga aaattgctgc aaaccgcgaa cggtcatgat
    50521 ttgccgatct gccgctgagc taactcctga aaatggaagc tcgcaggacg tgacaagggg
    50581 ccggttccgc gaccgcgtaa cctgatcgag gtgaggtgcc gtacgcatgg cggagcacac
    50641 ccgcgaagcc gacgaaaaga tcccttcgaa ttggaatcag aaaccgcatc cggcggcccc
    50701 cgacgacgag gagatgcccg cctcgatggg aggatccgcc ctgttcgccg aggaggagga
    50761 cgactacctg gacccggacg aggtgccggc ggcggccagt gaagagatgg acgcgatgat
    50821 cgatcagtac ctcgatcaga tgaccggggc gctgagccag gggcaactgt tgaccgttcc
    50881 ggtcgtcgcg atcaaatcgg atcacgtcct ggtggacatc ggtgagaagt ccgaggggat
    50941 catccacatt cgcgagttcc cgctgcgggg caacgtcccc gacgtgcaac tgggtcaaga
    51001 aatcgaagtc gtcgtcaagg gctacgacgc cgaatcgggg ctggtcaacc tgtcgtatca
    51061 ggaagcgcgc cggcgcaagg cgctggagga agtcaacgcc gcgctggagc agggccgccc
    51121 gatcaagggg accgtgaccc ggacggtgaa gggggggctg attctcgaca tcgggacgac
    51181 ggcgttcctg ccggcctcgc agatcgatct gcggcgggtg gagaatttcg aggaatggat
    51241 cggccgcgaa ctggaagcgc tggtgatcga gcatgtcccc gagaaacggc ggattatcgt
    51301 ttcgcggcgc cggctgctgg agcgcgagcg cgagcagatg cgccagcagg cgatgagccg
    51361 gttggaagtc ggcagcgaag tcgatgtcgt cgtcaagcgg atcgtcgatt tcggcgtgtt
    51421 cgtcgatctg ggcggcgtcg acgggctgat tccccgcagt gaagtttcct ggcaacgcaa
    51481 cgcccggccc gaggactact tcaacgtcgg cgacacgatc cgcgtcaagg tgatcgagat
    51541 cgaccaggag acgggcaaga tcacgctgtc gcggcggctg gcgatggccg atccgtggga
    51601 aacggcggtg gcgaaatatc cggcggggag cgagatccgg ggcacggtcg tctcgctgac
    51661 caattacggc gccttcgtcc ggctggagga aggcctggac gggatgatcc atatctcgga
    51721 catggcgtgg gacgcggccg gccacaagcc gggcgactac gtccaggcgg gccaggaagt
    51781 ctccgcgcag gtgctcaacg tcgaccgcga aggccggcgg atttcactcg ggctgaagca
    51841 gatgacgcgc gacccgtggg aggacgtggt cgagcgcttc cccaggggga cgcacatcaa
    51901 gggcccagtg acggggctga ccaagtatgg cgcgttcgtc gagctggagc ccggcatcga
    51961 ggggatgatt cacgtttcgg atttctcgtg ggacaagcgg gtcagtcagc ccaaggatgt
    52021 cgtgcaccgg ggcgaggaga tcgaggccgt ggtgctcgac atcgaccgcg aacggcggcg
    52081 gatttcgctc ggcgtcaaac aactgagtga aagcccgctg gcgctgttca aggcgcgcca
    52141 caaggtgggc gacattgtcg agggtgaagt gacggggatc accgagtttg gcgcgttcgt
    52201 gcggctggcc gatggcgtcg acggtttcct gcacgtctcg cagattgatc gcgaccgcgt
    52261 ggagaaagtt gccgatcaca tcaaggtcgg cgacacgatc cggaccaaga tcgtgaagat
    52321 cgacagcggc acgggcaaga tctcgctcag ccggcgccag atgatgaagg agcaggagaa
    52381 aaaggtcatc cagtcctacc tgaaacggtc cgggcgcgag gagagcatca acccgatggg
    52441 cgagctgctc gaggacatca acctgctgga cgatctgccc tccgagccgg agagtccgca
    52501 gccgcgggag gaagcgcccg aggtgccgat cgagcgggcg cagcgggttg atcgcgcgcc
    52561 cgaagaaaat gagaatcagt tggccgatgg gctgccggag gctccggggt ccgccggcgc
    52621 cccgcccgcg gcagggcccg aagaagagcc gcgcccgacg ccggaggata atgcgcaggg
    52681 gtgatgaccc gcgccccggc gtgtcgcgcg ccgggcgccg ggcgccgggc gccgggcgat
    52741 aaaactcgac aaatccgacg gattttccgc tcggccgggt cgctgccacc gcgccgctgc
    52801 cgacccgttc gcaagccttc gctccgcttc tgcgattttc attgttcccc ctcgacttgc
    52861 ttggtaaaag accggagccc gggccggcgc ccgtcgcggt cgggcttgcc catccgcctg
    52921 cccgccaagg agcccagcca tgcctgaacc gaagcccgcg ccggggcgca acctgacgtc
    52981 gcgattgcag ccgggacgct gccaggtcgg gacgatcctg agcctcgaca gtccggaagt
    53041 ggccgaaatc atgtcgcatt gcggcttcga ctggttctgg atcgacatgg agcactccgc
    53101 gctgagctgc agcgacaccc agcggctgat catggcgatc ggcgcgcggt gtccggccat
    53161 cgtgcgcatc ccggaaaacg attcggcctg gttcaagcgg gtgctcgaca ccgggtgcga
    53221 cggcgtgctg gtgccgatgg tgaacagcgc ggaggccgcc cggaaggcgg tcgatgcgtg
    53281 ccgttacccg ccgttgggca agcgcagcat cggggtcagc cgggcacacg gctacggcat
    53341 gaagttccgg gaatacgtgg acgcctcgta cgactccatc gacctgatca tccagtgcga
    53401 gcaccatgag gcggtggcga atatcgaatc gattctggag gtcgagggga tctcggcaat
    53461 tctgatcggg ccgtacgatc tgtcgggcag cttcaaccgg ctgggcgacg tcaagcacga
    53521 ggaagtgcag caggcgatcg acatggcgcg caaggcctgc aaggccaagg ggatgccggt
    53581 cggcattttc tgcatggacg tgaaaacggc gctcaggctg atccccgatg gttacgattt
    53641 catcggcgtc tcgctcgaca gcgtcatcct ggggcaggcc gcgcgcgcga tgagcgaggc
    53701 gctccacggg gaaggcaacg cctggcgcga atcctgaacc gcccggctgg atcgggccat
    53761 tggcctcagc cccctcctcc gtctcttcca tggagcggat gggcggacgc ccgccggtta
    53821 tgttaaggtg gacttggcgc cgatgtcggg tggaaggaat ggacgagggt gagcaagccg
    53881 acgattctgg tcatcgacga cgaaaccaat ctgctgcggt tttttgagta caacatccgc
    53941 ggattgggct acgacgtggt cgtcggggcc tcgggcgccg atttccagcg catgatcggc
    54001 gaacgggaat acgcgacgat cctgctcgat ctgatgctgc ccgacgccag cggcctggac
    54061 ctgctcgagc aggcgcgcaa aatccatccc gagctgcccg tgatcatgat cacggcctac
    54121 ggcacgatcg accgggcggt gcaggcgatc aagctggggg cgtatgaatt tctccccaaa
    54181 cccatcgatc tggacaagct gaacacgatc atccgcaacg cggtggagca gtatctgctg
    54241 cgccgcgagg tgcggacgct gcgccggcaa ctggagccgc cgcgcgaatt ccagtcgatg
    54301 gtcggcacca gcccggccat gctggaaatc tacaacatga tcgagaccgt ggcggcgacg
    54361 acggcgacgg tgatggtgac cggcgagagc ggcaccggca aggaactggt cgcgcgcggg
    54421 ctgcacaacc tctcctcgcg cgccggccgg gagttcgtcg cgatcaactg cgcggcgatc
    54481 ccccgcgacc tgctggaaaa cgagctgttc ggccatgaga agggctcatt cacgggcgcg
    54541 accgaacgct acaaggggtg tttcgagcgg gccgacaagg ggacgctctt tctggacgag
    54601 atctgcgaga tggacctggg actgcaggcc aagctgctgc ggctgatcca ggaacagcag
    54661 ttctaccgcg tcggcggcac cgagccggtt caggtcgatg tgcggattct gacggcgaca
    54721 aaccagaatc cggccgaggc ggtgcgggcg gggcggttcc gcgaggatct gtattaccgg
    54781 ctcaacgtgg tgccgatcaa cctgccgccg ctgcgcgagc ggcgcgagga tatcggccag
    54841 atcgccaaca aatacctgct ggagttctcc aaggcgtacg gccggcggtt tcagggcttc
    54901 gacctggagg cgctggcggc gcttgaacgc tatggttggc ccggcaatgt gcgcgagctg
    54961 cgcaacgtcg tcgagcaact ggtcgtgttg aatgaggggg agcgcgtgac ggcgtcgatg
    55021 ctgccggcgc ggattctgga agcgcggccg gcgccggtgg agccggggca agccgccccg
    55081 gcggggggcg agcgcgcgac gctgacctcc gaatcgatcc gcccgttctg gcaggttgag
    55141 cgcgaccaga tccagtcggc gctggatctg tgcggcggca acgtgcagga ggtggcgcgg
    55201 cggctggaga tttcgccggc gacgctttac cgcaagatcg aaaaatacgg cctcgtcaaa
    55261 tgagcacgcg gatggaaccg cgcccgcgac gcaccccgca gcccaccccg ccggtcgtcg
    55321 gcgactatct gtcggccgaa gactcgcacg agcattttct gaaacagggc cgccagttcc
    55381 tgcaggactt gcagcgctgg ctcgtggcca acaaccatct gcatcgctcg atggcgatca
    55441 ttcaggcgtt tacgcgggcc catgatcggc tgatccaggc ggcctaccag catgcctgcc
    55501 gccagtggag cgaggagacg gggctgatgc ccccgcgcgt ggcggtgctg gcgatcggcg
    55561 gctacggccg ccgcacgctg atgttgcgct ccgacatcga tctgatggtg ctgatggccg
    55621 agcacaccgg cgacaacgaa cggttcgtca aaagcctgat gctgacactg atcgatctga
    55681 agctgaacct gggctacgcc acgcggacgc aggcggactg cctggccgcg ctgggcaacg
    55741 acctggattc ggccacggcg atgaccgaaa gccgtctgct ggcggggcat cggccgctgt
    55801 tcaacgagtt catggcggcg ctggcggcgg cgctgcaggg gcgctggcgg cgctggttca
    55861 tccgctcggt ccatcagcag atgctcgaac gccgccgcaa atacgagtcg acggtctaca
    55921 tgctggagcc gaacctgaag gagggcaacg gcggactgcg cgatgcgcat acggtgatgt
    55981 ggatcctgtt cgcgctgacc gggtcgctca agctgcgtca gctcatcgcg ctggcgcgct
    56041 tcaccgagga ggacttcagg cgcctgcgcg aggcgctgac gatgctccgc gtcatccgca
    56101 atgaaattca tattaccgcc gagagcaaga ccgatcagtt gatctttacc tgccagccgc
    56161 tgattgccga gcggctgggc taccagtcga ccgacatccg ctcggccgaa gaagttttca
    56221 tggccgatta ctaccgtcat gcgcgcaccg tcgagcgcct gacgcatcgc gccgtccggg
    56281 tgctgctgca cagcgaccgt tcggtgtggg acggcgtggt cggctcgctc aagcggcggc
    56341 ggctggaccg gtatcatttc gtccaggaca acgtcctgtt catcgacgac gcgcaccagg
    56401 attatctgga acaggagccg cggcggattc tggagctgtt tgagcgggcg gcgaaatggg
    56461 gctaccggct gagcgaccgc acgctcgacc gcatcacgcg ggcggcggcg ggattggggc
    56521 cgggcttccg gctcgacccg gcgaatcacg cggcgttcat gcgcctgctg cgcggcccgt
    56581 tcaacgtcca ccgcgcgctg gccgacatgc atgagaccgg tgtgctcgcg aagttcctgc
    56641 ccgagttcga acgcctgcgc tacatggtcc gcatcgatca ttaccatcac tacaccgtcg
    56701 atgagcacac gttccgggcg ttggaggagg ccgagcggct gcagcgcatg gggagcggct
    56761 cgggggatca cgtggccgag gtcgccgcgt cgatcaagcg ctgggacctg ctcaatctgg
    56821 ccctgctgct gcacgatatc ggcaaggggt acggccgggg tcacgcgctg cgcggcgggc
    56881 acatcgcgca gcgggtcggc gaccggatgg gcctgaagcg cgccgatacc gatctggtgc
    56941 ggttcctcgt gctgtcgcat ctgaagttga cgcacgcctc gcagcggcgc gatctgtccg
    57001 atccggcggt ggcgcgcgcg ctcgccgagg agatcggctc gctcgagcgg ctcaagatgc
    57061 tctacgtcca taccgtctgc gatctgcggg cggtcagccc cgatgcgtgg aacgactgga
    57121 aggggcaact gctggccgaa tgctatgcgc ggacggcgga gatgctggcc ggcgagcggc
    57181 gcgcggccgg gggcgcacag ccggaactcc cccggctgaa gcgcgagatt ctggccctgc
    57241 tgctcgatgg ccaggccagg aaagcgggcg cgcggcgcgc cggcgacccg gagctggagc
    57301 gggacctgga cgagtttctg gggcatgtgt cccagcgcta tattcgcaac atgtcgcccc
    57361 gccagatcgg gcgccatttc ctgctccgcc ggcagttgca gggcgaacag cgcgtggcgt
    57421 gggagctcga cccgcggccc gaagggggct acaacgagat tctggtcgcc gcccacgaca
    57481 cccccggcct gttcagcaac atctgcggcg cgctggcggc caaggggatc agcatctggt
    57541 cggccgagat cttctcgacc aacgacggct atgcgctcaa tcagttccgg gtgacggacc
    57601 tggacaaccg gccgctggcg ggagggctgc ggctggaccg gctgcgcgac gacctggacg
    57661 acgtcctgca ggaacggaag actatcttcg aactgatcgc gaagcatcgc acgcggcggc
    57721 ggcgcaaggc ggtggccaag gcggtctatc ccaccagcgt ccggttcgac aacgattcct
    57781 cgcagcacca tacggtgatc gaaatccgct cgaccgaccg cccggggctg ctgttctgga
    57841 tcacacgggc gctcaacgcc agccggctcg acatccaccg ctcgatcatc gcgacggagg
    57901 cctacggcgt ggtggacgtg ttctacgtga ccgacctgga atacaacaag atccacgacc
    57961 ggcacgcgca ggagaagatc cgggcgacga tcctggatca cgtggagcag gcggcggaaa
    58021 tgtttgccgg ataaacccga tcggggtcgt ccctcgtgat cggtatcggg atcggtattg
    58081 ctatcgaacg acggtaagcc cttgtcgaga gccgaggacg agggccgaga gccgagagcc
    58141 gaattttgga gaaggccttt gggggcgatt acgagttgct cacggatctg cgcttcgcaa
    58201 cgagggatga aatggggggt gaacgatcca ggccttgccg gatccttcag cagggcagtc
    58261 ccatcgggga cggcagactt cagcctgctc cttcagtggc gggaaccggg atacccatcg
    58321 tgaccgagtc ccgtagggac gacagcaccg tttcaagtga tctcgcgatt agcggataag
    58381 ttttcgagtg aagataaggg atgtgggacg agcggggaga tcagcaactc gcaccaagtc
    58441 gcccgaagcg cactttccct gcttgaccgc tggacgccgg tcggatataa ggagaggcta
    58501 cgcttgacat tcccgggtgg aagatcaggc gatggctccc gcggccgctg ggcggcgggg
    58561 gcgcaccaag cagaaggcat cccgccttct cacccccagc gctccggcga cggagttgtg
    58621 cggtgggttc cagggcgaag cgaaccattg cgttggtctt cacccacagg ccggaatcac
    58681 gagcgcgcgg cgcgccgacg cgccgggatt cgggcatgga cggtccaggc ggccggggcg
    58741 ggattgtgag gcatttcccg tcgcggctgt tttgtttttg agccgccgca tccgccacgg
    58801 gggatgcgcg acgggatgca gcgagcgaac acgcgagcaa ttgagaacgt gactccccgc
    58861 cgcgacgggg tgaaaaggag gtaggcgcca tcaggaccaa tacgcgcacc aatgaggaga
    58921 tccgggtcaa ggaggtccgc ctgatcgacg aggcgggcaa gcagctgggg atcatgccga
    58981 tcgacaaggc gctggagcgg gcgcaggcca aggcgctcga cctggtcgag gtggcgcccg
    59041 atgcccagcc gccggtctgc cggatcatgg acttcacgaa gtatctgtat gagcagaagc
    59101 gcaaacagaa actggcgcgc aagaagacgg cgcgcgtcga cacgaaggaa gtcaagctgc
    59161 gtccgaacat cgacccgcac gatttcggga tcaagctgaa tcattcgcgc gagttcctgg
    59221 agaagggcaa caaggtcaag ttcacgatcc gctaccggcc gcgcgaaatg cgtcactacg
    59281 agatcgggct gaaggtgatg gaccggctgg tggaggagct gaaggaactg gcgagcgtcg
    59341 aaagcacgac gcgcaaccag agcggcctgc gcatgcagac gatgattctg gcgccgcgga
    59401 aggaagcgat cgcgcggcgc gatcatgagg aaggcgaagc ggccgagaat tgaacgagcc
    59461 gaccggcgga cgcgccgccc gggcatcaac cgtagacggg gagagagagc agcatcatgc
    59521 cgaagatcaa gaccaagggt ggcgtcaaga aacgcctcaa gccgacgggc ggcggccaca
    59581 tcaagcgcaa gcgctgcaac cacggccaca tcctgaccaa gaagagcgcc aagcgcaaac
    59641 ggcgcctgcg ccagacgacg ctggtgcatg aggccgacga gcgccggatg aagaagctgc
    59701 tgcacatcta gggggaagca acgcctccga cggtgacggc cgcgatcagc ccgccccgcc
    59761 gcccgcgcgg cgggaccgtc cagagccact ttgagagtcc ccggggggga cggcggaagc
    59821 gaaccggatc ggccggcggg ccgccggcgc gcatccgcac catagaacgg agtaagactc
    59881 atgcctcgcg taaaaaacag cgtcgcgacg cggcgccgcc gcaagaagat tctcaaattg
    59941 gccaaggggg cgcaggggag tcgttccaag ctgttcaaga atgccaagga gacggtcaag
    60001 cgggggatga actacgccta ccgcgaccgg cgcgtgcgca agcgcgaatt ccgtgcgctg
    60061 tggatcgcgc ggatcagcgc gggggtgcgc gcccacggca tgaagtacag cgaattcatg
    60121 cacgggatga agctggcggg gattgagctg gaccgcaagg tgctggccga cctggcgatg
    60181 aacgacgccg aggcgttcgg cgccatcgtc agccaggtcc gcaattcact cgcacaggcc
    60241 gcctgatgat ggctgcgacc ggcgtggaga cgttgcgcga acgactgcgc gaactgggcg
    60301 agcaggcgcg ccgccagatc gaggcggccg ccgacgccca ggcgctggag gcgctgcgcg
    60361 tgcagtacct cggcaagaag agcgagttct ccgggctgct caaggccctg gggcaactcg
    60421 gcgccgagga ccgcaagctg gtggggatgg tcgccaacga ggtgaaaacc gagctgggcg
    60481 acctgatcac gcggcggcgc gaggcgttcg acgaggccga gcggcagcgg cggctcgccg
    60541 aggagcggct ggacgtgacg ctgccgggcc ggccggtccc gcgcgggcat ctgcacccgg
    60601 tcacgcaggg gatccgcgag atctgcggga ttttcgtcca gatgggcttc gaggtggcca
    60661 ccggccccga catcgaggac gactaccata acttcgaggc gctgaacttc ccgcccaatc
    60721 acccggcgcg cgacatgcag gatacgttct acatgcggga gggcgggctg ctgcggacgc
    60781 atacctcgcc ggtccagatc cgcacgatgc gcgagcgcaa gccgccgctg gccgtggtcg
    60841 cgccggggca tacctaccgc tgcgacgccg attcgacgca cctgccgatg ttcacgcaga
    60901 tcgaaggctt catcgtcgat gagaatatcc ggatgagcga tctgaaggga gtcctgcagc
    60961 ggctgatcca cgcctacttc ggcgcggacc ggacctaccg gatgcggccc tcgttcttcc
    61021 cgttcaccga gccgagcgcc gaagtggaca tccggtggga aaacgcggcg ggcgaacagg
    61081 tctggttcga ggtgctgggc gccggcatga ttcatcccgc cgtgctggag aacgtcggct
    61141 acgatcccca gcgggtcagc gggttcgcct tcggcctggg cgtcgagcgg ttcgtcatgc
    61201 tcaagcacgg tatcaagaac atcaagcacc tctacgaaaa cgacctgcgc tttctgcggc
    61261 aattctgatc gaagcggcgt tgcgcccggc ggcgcgggac gcggcttcag cgggccccgc
    61321 cggaacggat gccttcgccc gcaactgaga agggaatcgg ggaagaacac cgatgaagat
    61381 ttcggttcgc tggctgagac aattcctcaa tgtcgaactc tccatcgccg agctgaagga
    61441 gacgatggag atggcgggcc atgaggtcga aggggcatgg gatctcggcc tggaatcggg
    61501 caagctggtg gtcgcgcggg tgctcgacgt ccaactgcat cccaacgccg atcggctgcg
    61561 ggtgtgccag gtggatgcgg ggacgggcca gccgctgtcg gtcgtctgcg gggcgcccaa
    61621 tgtgacggcc gggatgaatg tcgtctgcgc gctgcccggg gcccggctgc ccgacggcaa
    61681 cacgatcaag aagagcaaga tccgcggcca actgagcgag ggaatgctct gctcggcgcg
    61741 cgaattggaa atggggaccg atcactccgg catcatgccg ttgcccgagg atctggagat
    61801 cggccagcca tacgactaca ttatcgacat caaggtgacc cccaaccggc cggactgcct
    61861 gagcatcctg gggatggcgc gcgacatcgg ggcggcgctg ggcaagaaag tcttcccgcc
    61921 gacgccgcgg ttcaaggaga cgctggagca catcgacagc ttcgtgcggc tgagcgtgaa
    61981 ggaccgcggc gcctgcccgc gctacacctg tcggctgatc cgcggcgtca aggtcggcga
    62041 gagcccgctg tggctcaaac gcgcgctcga agcgtgcggg atgcgttcga tcaacaatgt
    62101 cgtcgacgtc acgaactacg tgctgatgga gctgggccat ccgctgcatg cgtttgattt
    62161 cgaccggctg cacaacagcg agatccaggt gcggcgcgcg gaggcctgcg agcggctcgt
    62221 cctgatcgac gaccgcgaga tcacgctcga tccggaggat ctggtgatcg ccgacgccga
    62281 ccggccgctg gcgctggcgg gcgtgatggg cgggcgcgac agcgaagtgg ggccgggcac
    62341 gacgacgatc ctgctggagt cggcctattt tgaccccgcg acgatccgcc ggacggcgcg
    62401 caaatacaat ttccagaccg aatcgtcgta tcgcttcgag cgcggaaccg accgcgcgcg
    62461 gctgacactg tcgctcaatc gcgcgaccca gctcattcac gaactcgccg ggggcgaggt
    62521 gatcaagaac atgctggacg cgcagacgac catcaccgac cccggcccga tcgtcctgga
    62581 tgtccggcgg gtgaaccgga tcctcgggct ggaactgacc agtaccgaga tcgcggacta
    62641 tctggtcaat ctgggctgcg aaatccggcg cggcgacaag gaatcgctcg tggtgtcaat
    62701 cccgagccac cgggtcgaca tttcgcgcga catcgatctg atcgaagagg tcgcccggct
    62761 tcacaactac aacgagatcc cggcgacgat gcccaaactg atcccgacga tccggccgtg
    62821 tgaccggttg ggcgagctcg ccacccggct gagcgatcag cttgtcgcgc tgggcttctg
    62881 cgaggtgctg caattcagct tcatctcgga ggccgacgcc gcggcgggcg gcttcaatat
    62941 cgagcggctg ccgcgcctcg ccaacccgct gaccgtcgac caggcgctga tgcggccgtc
    63001 gctggccacc ggcctgctgc gcgtgatcgc cgccaaccag aaggtcggcg aggcctccca
    63061 gctgctgttc gagatcggaa aggcgtggcg cgaggatgcg cgcgcggccg accccgccgg
    63121 cgagcgccac gaactggccc tggcggcggc gggaccggtc ccgacgcact ggggcgcccc
    63181 ggcgcgcgag tatgatttct acgacctgaa gggggtcgtc gagaatctct tttcctggat
    63241 caacggcccc gcgccctcct tcgcgcgcga cgccggcaac ccgctgctgc atccgggccg
    63301 ctcggcgcgg atcgaatggg acggtcggcc ggtcggcgtg ctggggcagg tgcacccgcg
    63361 cgctgtggcc cagttcgatc tgcgcggcga cgccgtcctg gcgctgatcg atctgagtct
    63421 gctggtgagc ctcctcggcg aatcggtggt ccagttcaag ccgatcccga aattccccgg
    63481 cagtcttcgc gatctggcgc tggtggtgga tgagcaactg ccggcggcgg aaattttgag
    63541 tctgattcac gaggccggac ggccgctggt ggatgaggtg caggtcttcg acctctacag
    63601 cggcagtcat gtccccgcgg gcaagaagag cctggctctg cggctgcggc tgctctcgcc
    63661 ggagaaaacc ctgaccgagc aagagatcac cgaaaccatc gaacggatcg tccggcggct
    63721 tgagcagcag tttggcgcga cgttgcgcac ctgaaaccgg caatcgccac cgcccatgga
    63781 agggggcagc gtcatggccc gccgaaaaaa aaccgagccc gaaccggaac cggaactgat
    63841 gcccagcttc tctccgcgga tcatgcgcag acgactggag gcgttccagc gcggagtcca
    63901 tcggctgatc gccgagcgcg gcttgaacac ccaggaggag atccaggcct tcctggaaca
    63961 gcactcgacc gacggctcac tggaacggat gatcgacgag ctcgatctga tgcccgagga
    64021 ggaagcgctg gagctggttt ccgaggcgat ggagtgcgag gatctggacg agatgctgga
    64081 cctgttggat gcggcgctgg aagccgatcc ggagtgcatc gaggcgcttt gtctgctggc
    64141 gcggatcaat tgcgaggaag actcggaggc gcttgtcgat gcgctggaag acatcgtcaa
    64201 gctgggggag gaaaaactgg gggccaaaat gttcgaagag tgccgcggcc agttctgggg
    64261 tctgttggaa acgcgtccct acatgcgcgc ccggcaggaa ctgatcacgg cgctggcgat
    64321 ctgcggacgg ttcgaagagg ccgcggcgca tggcgaggcg atgctcgagc tcaacgagga
    64381 cgacaatcag ggcatccgct ctgagctgct ggtgcactac ctgctgctgg ggaatctgga
    64441 aggggcgcgc gcgctgttga agcgctacga ggaagtgacc gcggtcttcg cctgggccca
    64501 cgtgttcgag cgcttcctgg cgggaaacct cgaggaggcg cggtccgcca tgaccgaggc
    64561 tcgccgcgtc aatccctacg ttaaggaata cctgacgggg aaagccgcat tgcccgacct
    64621 gctgcccgcc tatagcactc cgggcgatcg cagcgaggcg atcggctgcg cggcgatgct
    64681 gggccgcgtg ctgctgcagc gtcccgagct ccacacctgg ctcgtcaact ccctgaagta
    64741 acgcggcggc ggcgtcccct gtcggacgcg ccggcgaatt caacctgctc atgcaccacc
    64801 ccaccatccg cacccgcatc gcgccctctc ccaccggcga tccgcacatc ggcaccgcct
    64861 atatcgcgct gttcaattac gttttcgccc ggcgtcacgg cgggcggttt ctgttgcgaa
    64921 tcgaggatac cgaccgcgtc cgctccacgc cggagtccga acgggccatc ctggcggcgc
    64981 tgcgctggat cggcctggcc tgggacgaag gccccgacgt cggcgggccg cacggcccct
    65041 atcgccagag cgaacggacc gaaatctacc gcgcccactg cgagcggctg atcgacagcg
    65101 agcatgccta ttactgtttc gcgacgcccg aacagctcga cgcttaccgg cgcgagcgcc
    65161 tgcaggccgg caaggcgaca ggctacgacg gatcgttcgg gatgtggacg cgggccgagg
    65221 cgcgcgcgcg catcgccgcg ggcgatccgc atgtgatccg gctgagggcc ccggccgagg
    65281 gagagtgcgt gatccacgac cgcctgcgcg gcgcgatccg catcccgtgg gaaaacgtcg
    65341 atcatcaggt cctgctcaag gccgacggcc atccgaccta ccacctggcc agcgtcgtcg
    65401 acgaccatct gatggagatc acccacgtca tccgcggcga ggaatggatc aattccgccc
    65461 ccaagcacaa gttgctctac gaggcgttcg gctgggagat gcccgagctg atccacatgc
    65521 cgctgctgcg caaccccgac cgcagcaagc tgtccaaacg caaaaacccg acgagcatcg
    65581 actactaccg ccaggcgggc tacctgcccg aggcgctgct gaactacctg gggctgatgg
    65641 cgtggacgta tccgcccgcg ccgggggaga ccgaggggcc ggagatgttc agcctggagg
    65701 aaatgatcgc ggcgttcgac atcgaccggg tgagcctggg cggaccgatt ttcgatcccc
    65761 ggaagctcaa ctggctgaac gggcgctacc tgcgcgagaa gcatgacccg gcggcgctgc
    65821 tggcgcgact gcgcgactgg atgctgaacg acggggtttg gctgcgcgcg ctgccgctgg
    65881 tgcagccgcg cgcggagaaa ctgagcgacg tcgtgccgat gaccgcgttc ctgtttgccg
    65941 accggccggc gtatgacccg cacagcttga tccagaaaaa actggacggc gacgcggcgg
    66001 cgcggctgtt gcgcattgtc ctgtgggaac tggaacgggt gacgccgtgg aacaaggaga
    66061 cgaccaaggc ggcgatcagc cggattgccc agatcgaggg actcaagctg cgcgagctga
    66121 tgccggtgct gttcgtcgcg ctgactggcg cgccggcctc gctgccgctc ttcgacagca
    66181 tggagattct cggcgccgcg atgagccgcc ggcgcctggt ttacgcgctc gaggcgctcg
    66241 gctcgatcgg ccatgaactg agcagcaggc agctcaaggc gatcgagcag gagtatcgcg
    66301 agaaatatct ggagcagggc aagggcgaga cgcaggccgc cggcgacgga gcggacggat
    66361 caggggcgga agaatagacg acggggaatc tcgagagccg aggacggaga cgagcgacgt
    66421 attcttgatt taaggaaggc gcgcgatggc ggccacgcat ctgcattttg actgcacgtt
    66481 cgggatcagc ggcgacatga ccgtcggcgc gctggtcgat ctgggcgtcg cgctcgaggc
    66541 gatcgccgac cggctgcgcg acctgccgat cggcccgttt cacttgcgcg cggaaccgct
    66601 gctgcgccag gggattcgcg gcacgcgcgt ccatgtcgag gtcgccgagg acgaaaagcc
    66661 gcaccggcac ctgcgccacg tgctggatat cctcggagcg gcctcgctgt cggcggcggc
    66721 gcgccggcgg gccgaactgg cctatcgccg gctggccgag gccgaggccc acgtgcatgg
    66781 ctccacggcc gacaagatcc acttccacga ggtgggcgcc aaggacgcga tcgtcgacgt
    66841 cgccgccgcg atggtggcgg tcgagctgct cggcgccgag tcgttctccg ccggaccgat
    66901 ctacgtcggt tcggggacga ttcagtgcca acatggcgtc atgccggtgc cggcgccggc
    66961 cacggcggag ttgctgcgcg cgctgccgca tgccgccggg ccgtttaccg gcgagatggt
    67021 gacgccgacg ggggccgcga tcctgcgcgc gctgctggag gacgcgggcg agccgccgat
    67081 cggcggacag gaggtggaat cgccggcgcg ggtggttcag cggatcggct acggcgcggg
    67141 gacgcgggaa ctgccggggg cttccaatta cctgcgcgtg ctgctgtgcg aatcgccgcg
    67201 cgccgccgcc gggggcgagg agggcgacgc cctgccgctg caccgccacc agattgtggt
    67261 gctggagacc gaaatcgacg acatggcccc cgagacggcc ggctacgtga tggagcggct
    67321 gctggcgcgc gaggcgctgg acgttcagtt ttcacccgtc cagatga
//
LOCUS       DAOPCO010000002        57248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_636_length_57248_cov_5.638802, whole genome shotgun sequence.
ACCESSION   DAOPCO010000002 DAOPCO010000000
VERSION     DAOPCO010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 57248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57248
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_636_length_57248_cov_5.638802"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1009)
                     /locus_tag="PLS90_00290"
     CDS             complement(17..1009)
                     /locus_tag="PLS90_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK00871.1"
                     /translation="MSKVKVALIGAGGMANTVHYPSLTTMDDVEITGLCDLNEQRLKE
                     TAAKFKIGKTFTDWRKMLDETKPAAVYVLMPPHHLFDIAMEALERGHHLFIEKPPAVT
                     TFQADCLARMAEKKKLISGVGFQRRYHPLVRRCWEEVKKKGPVHQVNSCFYKDLPPQD
                     KHPYYGGAIDILHCDAIHAVDSLRYYAGLSEVKAVASEVRTIDSWYAVSFNSLVYFEN
                     DCVGILQANWRTGRRFFKFELHAPDASAYLDIDAEGVVWTDNGAEPTLKLTNTEAAQS
                     QEFHVFQGFFAQSRAFIDAVASGKPLHNDLRDSVKSMELVDLIYQNAINKKCCR"
     gene            1376..2113
                     /locus_tag="PLS90_00295"
     CDS             1376..2113
                     /locus_tag="PLS90_00295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPK00872.1"
                     /translation="MKRDRRAADFRRLVTLGESTTAGGWASHRGRCWASLLEQKINLF
                     QTLPVQLFNSGLGANLVTREAPAYPHSGRPAADERLERDVIRHEPDLLILAYGLNDAR
                     GGTSLPLFIATLASLINRVRERLDPLIVIVGSFYACRFRYDDPNWEHADLIGLRQFSD
                     ASRGVAEDHDCLFVEMISAFDGADWLMHYDGVHANDLGHQVIADRIFGVLAANCTCLA
                     TRTKALEPQIAPWRDESTLRTPILPHA"
     gene            complement(2286..3851)
                     /locus_tag="PLS90_00300"
     CDS             complement(2286..3851)
                     /locus_tag="PLS90_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPK00873.1"
                     /translation="MPFVDSSLGFWVVLIVIVIVLLFSTIAFLASRYRRCPSDRILVI
                     FGKVGTGQSARCIHGGGTFVWPLIQDYMYLSLVPMTISIPLKNALSLQNIRIDVPSTF
                     TVGVSTQPEIMNNAAERLLTLRRQEIEDMAREIIFGQLRLTVASLTIEQINQDRESFL
                     EAIRKNVEPELNKIGLYLINVNITDITDESTYIESIGKKAAAEAINQAKIDVAVQDKI
                     GSIGEAEAVREREIRVAQNVAEADKGRKAAEADQRVFVQQQETTATIGEAEANREKEI
                     RVAENIAAADKGRKAAEADRRVFVQQQEADAVKGENTAKANIAAYNAELAIKQAEATR
                     RGEVARREAEVEIQKAQARAEQERLTAEEVVRQEIERRKIEIAAEAEAEKRRREARGD
                     ADAILARYQAEAAGTRQVLQAKAEGYAALVKSCGGDANAAATLLMIEKLEEIVARQVE
                     AISKLKIDKITVWDSGGGNGRGGSSTAGFVSSLVGSLPPLQEVARMAGVELPQFLGKM
                     KDVDGGETDVAQG"
     gene            complement(3891..4472)
                     /locus_tag="PLS90_00305"
     CDS             complement(3891..4472)
                     /locus_tag="PLS90_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00874.1"
                     /translation="MVDLFWISLSGVDRVFLICTLIGGLLFIARGVLGLFGLGDHGAD
                     VGVDLSMADLTEGADLSFKVLTLQGITGFFLIFGLTGLALHRGSRVGELASILGALVA
                     GAAMVLLLAKLTQMMQGLHSSGNIDMRQAAGQSALVYLTIPPRGTGQIQISIQGRSRI
                     VDAMSKEDEPIKTGERVEVVEVVNSGLMLVKRA"
     gene            complement(4569..5240)
                     /gene="rsmG"
                     /locus_tag="PLS90_00310"
     CDS             complement(4569..5240)
                     /gene="rsmG"
                     /locus_tag="PLS90_00310"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPK00875.1"
                     /translation="MATLVELILAENTRQNLTADKTPELFWPRHIEDAVRAAAALHAR
                     VAALAAPRILDIGAGGGIPGLVWSLLWPSAKVVLLDSRRKRTAFLERAARELNLPCVD
                     VLTGRAEELAHDARHREAYDLATARAVAPLPVLLELALPFAAVGGAFAAIKSADQTME
                     LAAAAGVPERLGARAAGELDYRRSDGKACRVLIYQKRGPTPSRFPRNGAQLARRPLGP
                     AMDSA"
     gene            complement(5429..6331)
                     /gene="nadC"
                     /locus_tag="PLS90_00315"
     CDS             complement(5429..6331)
                     /gene="nadC"
                     /locus_tag="PLS90_00315"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPK00876.1"
                     /translation="MAPNHETVNRAQPQLQLTPELTRLLRAALKEDLGPGDVTTERTV
                     APRVRGRGELIAKQAGVLCGAPVAAAVWRLADRGVKIQWHCRDGERVRAGDRVATLEG
                     RMRGLLVGERLALNFLQRLSGIATLTCRFHDAAGGANGPLICDTRKTTPLWRALERYA
                     VRVGGGMNHRFGLFDMVLIKENHARAAGGLREAIRAVKAPGAQDGRKRLKVMAEARDA
                     EEVAICCAEGVDLILLDNFTPARARAVIARFRSCGIPFEISGGVTLKNVAAYARAGAD
                     RISIGALTHSAPALDLSLQLYPVE"
     gene            complement(6315..7115)
                     /gene="panB"
                     /locus_tag="PLS90_00320"
     CDS             complement(6315..7115)
                     /gene="panB"
                     /locus_tag="PLS90_00320"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPK00877.1"
                     /translation="MRVTHFAAMKREARRIAVATAYDAITAAWCERAGIDLILVGDSL
                     GNTALGYETTIPVRLEAMLHHAAAVARAARRAFLVGDMPFMTCRVSTEQTLVNCARMI
                     QEGGMEAVKLEGGSEVAPMVERLVTAGIPVMGHIGLLPQSVHQRGGYRVQGRDQQTAR
                     QLRDSARDLELAGAFSIVLEGIPAPLAAEITRSIQIPTIGIGAGPDCDGQVLVLSDLL
                     GMIDGPRPKFVKSYATLHDQAAAALAAYADDVRAGRFPTAEHSYGAEP"
     gene            complement(7352..8269)
                     /locus_tag="PLS90_00325"
     CDS             complement(7352..8269)
                     /locus_tag="PLS90_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPK00878.1"
                     /translation="MAREYHEHPTDLMSDARLWWAVAVNLLLTVAQVIGGGLAGSLAL
                     VADALHNLNDAASLGIALAARRISRRPPDRLRTFGYRRAQIIGALINLTALILVALYL
                     VFEAVLRWFERNPIDGWIVVWLAGLALAVDLATALLTYTMSRTSLNIKAAFIHNLSDA
                     LASVGVMLAGTLILLYEWYWTDLAVTLLISAYILWQSVGMLKETIHILMQGTPSDVRA
                     EEIAAALETVAAVRELHHVHVWQIDEHHRSFEGHVVIGEASLPEMECIKRDLRAILAE
                     RFQIQHSTLEVEIAEQLNGIACARREHAH"
     gene            complement(8354..11455)
                     /locus_tag="PLS90_00330"
     CDS             complement(8354..11455)
                     /locus_tag="PLS90_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008098670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HPK00879.1"
                     /translation="MLKYIQTILATRWLMLALGVLVVAGGWFSYERLPVDALPDVTPA
                     LVQVFTLTEGLAPEEVEKFVTYPVESVMNGLPGVDHIRSVSNFGLSVVNIYFEDGTDL
                     YFARQLVGERLGEAREQIPEGFGEPRMGPITTGMGQILFYRLEDATGRYSPTEMRTIQ
                     DWIVKYNLQTVKGVTEVLSLGGQVKQYQILIRPADLLRYGLTIHEVVESVEANNSNVG
                     AQFIIKNSEQYIVRSVGLAEDLDDLESIVLKTVDGTPVYLRQVADVVIGGEPRQGLAT
                     ADGRGEAVVGMVLKLIGTNTSEVIARVKARLDEINRILPAGVRIIAYYDQATLVENVV
                     NTVTTALVQGIVLVVIILLAFMGGLRPSLVVALSIPFSILFAFMLMKLLGISANLMSL
                     GGLAIAIGMMVDGTIVMVENVDRVLRESDPDESRLGVVTRACAEVGRPIFFAIGIIVL
                     VFLPLFTLQGVEGKTFRPLAYTISLAMFGSLLFAMLLAPVFAYCFMRPPRRARRDHKP
                     AGENWIVRLLLYPYRPVVGLFVRGRVLAVLLAVGIFALGLAIFPLLGSEFLPRLNEGD
                     LLIRATMAPSISLEEAGRTITRFEQDLRARFPEVRQVVSRVGRGEVGAHADPVNNAEI
                     FVELRPRAEWTSAATPEALMQMMSEAFEDYPGAQFNFTQPIAAAVDELLTGTKAELAI
                     KLFGPDMEVLAAKADEIAAVIGEVPGAADVQKDQVTGTPQVRIRMRYDQLERYGLNVR
                     DVQEVIRAAIGGAEAGQIFEGVRRFDITVWYHPEHRASIEAIRRILIPGPGDLRIPLE
                     DVAHVEEIVGPRQITRENNQRFITVQTNVRGRDIGSFVEQGRAAIAARVDLPPGYLVT
                     WGGQFELQQQANRRLAIVVPVTLLLVFLLLFSSFNSIRNALLILLNIPLALVGGVVGL
                     WLAGLNLSVPASVGFIALFGIALENGMVLVTCLNQLVREGLPMAEACVRGACLRLRPV
                     LMTAATTALGLIPLLFSSGTGSEVQRPLATVVIGGLVSSTILTLLVLPALYKWFSIPP
                     QRG"
     gene            complement(11497..12843)
                     /locus_tag="PLS90_00335"
     CDS             complement(11497..12843)
                     /locus_tag="PLS90_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK00880.1"
                     /translation="MRSKALIAALAAMLAIGVAAGIRSGTLARISIGFSAQAQEDAHH
                     DHADHEHERRSAAETGHVHEAAHTHEEHAHDAHGHDEHDHDAHGHGAHEAVHAVSFTP
                     EQLERLGIQVATATSGTLEAYAEFPGEIRLNEDALTHVAPRVAGIVREVNATLGDAVR
                     QGDVMAVLESRDLADLKAEYLAARERVALRELTYEREIALFDKQLSPEQEYLDARQAV
                     REASIELNAAGQKLRALGFSEDYLQALSNRPDAQLTRYTLIAPADGVVIEKHITRGEV
                     LDEASQPFTIADLSTVWVDLEVYQKDLPRIREGQQVVISAGEATAPAVGQIGYIGPIV
                     GEQTRTALARVVLPNPARRWRAGMFVTGRVAVDAVPAPIVVPKTAIQTMEDAQVVFVR
                     GAEGFAPAPVLPGREDQDEVEIREGLRAGQQYAATGAFTLKAELAKGSFDAHGHAH"
     gene            complement(12840..14282)
                     /locus_tag="PLS90_00340"
     CDS             complement(12840..14282)
                     /locus_tag="PLS90_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK00881.1"
                     /translation="MRLPLWLGLLAAIAPVAGCLRLAPAPYTASRPAPCPPGQGSAET
                     FARVSLPEPAAEYQTLPIEAPTGVLTLADAINRALDRHPELAALEQEVAAREAEVLQA
                     DLPPNPELEAEIEDLLGGTSGAEPEQQDLSGFTGAEATLTLSQTIERAGKRLKRRHLA
                     ERQADAAAWDIEARRLELLTRVQAAFVELLAAQEKVNLARQLAEIATQAHETVARRVE
                     AGKISPIEEKQSRVAWEVQRTDLAAAEAELDAARRRLAALWDAEPVFGEAVGDLERLP
                     PLPAAADLETLLADNPEVARWATEMAVRRAALELAEAEARPDPTLRVGARYFNETENH
                     ALILGFSIPLPLFDRNQGQIAAARHRLEQAEAEQAAARRRLREQLIELHGRAAGARDE
                     AERLRTVVLPDAQAVYESLLEGFWLGEYDLINVLDAQQRVFELRGRYLDQLTEFQQAA
                     AELEGLIGAPLATVAADSAGANDRIEETKP"
     gene            complement(14383..14838)
                     /locus_tag="PLS90_00345"
     CDS             complement(14383..14838)
                     /locus_tag="PLS90_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00882.1"
                     /translation="MLSRLRHARFIWLLPLLYLFGVGGAAHGFVLCLDEDHLAVELSH
                     QPERFCAPHCAPGPAAADERARVITGADPHEGCLDLLLIGGHAQPVPVKIERKGCEAP
                     APPVLASVDMPRREAGLFTGQRGPTAIDSSAPRCAPLALQLLRTVILII"
     gene            complement(14907..15542)
                     /gene="folE"
                     /locus_tag="PLS90_00350"
     CDS             complement(14907..15542)
                     /gene="folE"
                     /locus_tag="PLS90_00350"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPK00883.1"
                     /translation="MPKKHPQPQPIERVGLDEDLADEQANPRIAEATRMLLTEIGEDP
                     RRQGLARTPERVARAWEYLTQGYRTDLHTIVNGAIFDEKYDEMVAVKSIQFFSLCEHH
                     LLPFYGKCHVAYIPDGKVVGISKIPRIVDMFARRLQIQERLTAQIAESLHEVLSPKGV
                     GVVMEAYHMCMMMRGVEKQDSLTTTSEMLGVFKTSPQTRAEFLSLMGIKIA"
     gene            complement(15601..16680)
                     /locus_tag="PLS90_00355"
     CDS             complement(15601..16680)
                     /locus_tag="PLS90_00355"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HPK00884.1"
                     /translation="MTLSPVFRSERAVRLLEEHGIWGRLSSAAAQQAEGVQWVAAELG
                     ELISERHLAPPADDRPLTPGDGPLSFMQEYFFLTLFLSLFESLGISPDRFPFYARLNF
                     CIKGTITAADNLFDGQSKSLLPLREVEGACFGSILQLMCFERLLRRVGDRAVNDGLFS
                     ATAFAEVQRGLLDRMAEIGLLEGSEEAGVSEIPTPRQMIDHVHRVRGGMLFGLAFVAP
                     AILERGAIGERVAAAEPAIAQLGTAFQIVDDLTDFEFDLGRRSHNLLVAQIHHHGSDA
                     ERARLARLWEAREAGDGLVEEHFADSARTVLELAREEARASFATLAQLGFWFPPDLAD
                     EVVHAIVGIEGVARMESLTTARGNG"
     gene            complement(16886..>18403)
                     /locus_tag="PLS90_00360"
     CDS             complement(16886..>18403)
                     /locus_tag="PLS90_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00885.1"
                     /translation="AAESPEAAADATVAGEEPPTVEEAGDGQLIAMLTPTPGPEPATL
                     LPTPAPPAAPLLPPVPAQGRPWQGILITVVVVVSLIFIALVWMVVRRGGEEEELVLEG
                     DGTLTPEEQTGADPITAALAAQRSGSVDALEDQIYPLGETPPDSLAAEASDILPPVES
                     EFEIELEPMRTELPRDEVSAAPEPIDAVSEVEDAFVLPLPRESAIEPAAEEEPHPTEG
                     IEPLILHPDQLPATGSDEEDRIPPDAVISLEDEEVAPDAQREPTARYEQTDLLEVDAG
                     EAAPTPSSTPKAEPPAAEPDAPWQSDDLLEYDADAAEALAAEDGPEADQTLAGGLLDE
                     GEGEEDEVSAFEVTHTSWQTELEDTQSLGEVIDGEQRPQPGDIFHSDETVEIHLERMP
                     DAPPAADETAFNLERDRETLLPADELVELAPSQAETVAPTPEPAAPPVTAARSEAEEL
                     FQQECRKGLEEFRQARWSEAVHHLSIAAALRPEAQEVKEKLRIARRNKKQQEQGV"
     assembly_gap    18404..18503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18504..19134)
                     /locus_tag="PLS90_00365"
     CDS             complement(<18504..19134)
                     /locus_tag="PLS90_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00886.1"
                     /translation="MLLSVALAGMRVAVAQPTEPMDAATGLLPLPAVQYEDQSSTPVV
                     IDAPGPQPLSAAEAEAFQEEIRRAFVMQQYQRVLDLGEQLQNGPGLPQSADFYMAASR
                     MRLKERRDAEEGRSPFRRLSTQRPALEIPESSPESAEPGGELPATDEESAAAGAPDEP
                     AAESGAASAPDAVPPRAILRTQPPGAPSPAEAAEALADAIETATAAESPE"
     gene            19656..20627
                     /gene="cysK"
                     /locus_tag="PLS90_00370"
     CDS             19656..20627
                     /gene="cysK"
                     /locus_tag="PLS90_00370"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007363414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPK00887.1"
                     /translation="MARLFESIVDTIGHTPLVKLRRVAEEAGARADVYLKLEFFNPLG
                     SVKDRIGAAMIEAAEQAGKIEPGKTVLIEPTSGNTGIALAFVAAAKGYPLILTMPESM
                     SIERRKLLKILGARLVLTEGSKGMKGAIEVAEDLHRKIPNSFIPQQFANPANPDIHRR
                     TTAQEIWEDTDGRVDVVVSGVGTGGTVTGVGEVLKQKKPSVHIVALEPVTSQVLAGGE
                     PGPHRLQGLGAGFVPEVLNRGVIDEIIAVTEEDAARMNREITRKEGIPIGISSGAAAW
                     AAVEIAKRPEMAGKLIVAVMPSASERYLSAWIYNDIDLESDSIDDLL"
     gene            20643..21926
                     /locus_tag="PLS90_00375"
     CDS             20643..21926
                     /locus_tag="PLS90_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00888.1"
                     /translation="MIFFLHLLWRHVRQYPTRALAAVVVLTAASTIMLTLLGLGVALR
                     FRLGDYLTELFPEEQLRLEARRANLGYIALENNPITPEKVAELKSLPGVNQVWSVEPL
                     RFPIYATGSILGQELASDAVVHGVPRALVADSLPPDAPWRMPDDPDAPCPVIVSSYFL
                     DLYNLGLARSSGLPALSPSALVGRDFTFVMGESVLGLGLASDVTPVQVRAKVVGLSKQ
                     PGMLGLAIPADIARAYNHKFAPGTPETYAQVVVQTARDADRQAILARAGEMGLQLAGG
                     ELLGEQLKFGVRLTGWAVIALALAIFALGMLTFYLLFTMIFHARRPDLIALRTLGLTP
                     AQAVGLALGEVILLAATAVALALAATAGLAAWAGATMARWTEHLSWLPEDLLRPQPGW
                     LLAGAALILGVTLLLATPMLRWVVTVRPAAVIRDR"
     gene            21983..22717
                     /locus_tag="PLS90_00380"
     CDS             21983..22717
                     /locus_tag="PLS90_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPK00889.1"
                     /translation="MCGRFAVYSTIEELAELIREALPDLEIEQARGFVPSYNIAPSAA
                     IVTIGRSRPEHPHRMALLKWGLVPSFAKDPTRMAPINVRSETVLTKPMFRRPIERQRC
                     LVPANGFFEWKRAGSRKQPYFIRRADGRPLLMAGIYDVWRGGEGGAPLMTCAILTTDS
                     NDWMKPIHHRMPVMVAPEAAARWCDPQRPAADVLPLLTPSPADALEAYPVDARMNSPR
                     HDDPACIAPLPPAPDVPGDKVDPEQR"
     gene            complement(22739..23074)
                     /locus_tag="PLS90_00385"
     CDS             complement(22739..23074)
                     /locus_tag="PLS90_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00890.1"
                     /translation="MSSWRDKAIELLEPSVFDGDPLGAGADYVPDLIECYLQTGRTAR
                     AAEAMNVLLRPGAGTRARVWRAVEAYRRIDPFAADRLWERIRDVMAAPVVQLPLPPAP
                     GEVGTAAAD"
     gene            23297..24520
                     /locus_tag="PLS90_00390"
     CDS             23297..24520
                     /locus_tag="PLS90_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK00891.1"
                     /translation="MTALLRRFTQTPSAAPPADPRLHVALDVRFWSMSGIGTYVRELL
                     AAYARLRLPLVLTLIGPEGLRDRIAPGLEIRRWIHFEPPVYSAASFFRYPALPGVDLL
                     HYPHYNLPLTRARHKLVTFYDLFHLRYGSLPKRRYQDFFLTRLRWSRAHIVVPSEKTG
                     HDLEVAGLRGRRVSCIPLGPGQSPPERPATPPEDLLTLTGAPPRRRWLLTTGIDQPHK
                     NIDFLISALSLYYQRRPDAPPLVWCGLSAEDRDRRARQIPAHMRQHVVLEPFIDAERL
                     ESLMAGALALVFPSLDERFGFPPLEAMTRGVPVLCARREPMSTILGNAPLYFEPNESA
                     SLWRAIDRLLAPGTVRQELIDRGRHRAAQYSWDLTAWDTARLYYEVAGREVTVEGEET
                     PEPDDAAAVEQPRQA"
     gene            complement(24604..25899)
                     /gene="purB"
                     /locus_tag="PLS90_00395"
     CDS             complement(24604..25899)
                     /gene="purB"
                     /locus_tag="PLS90_00395"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006323587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPK00892.1"
                     /translation="MIERYALPEMRALWSDQTKFEIWLQVELLACEAWAQQGAIPAEA
                     LAQIKAKAAFTLERIDAIEAEVHHDVIAFTTALAEQIGEAARFVHMGLTSTDVVDTAQ
                     SVRMARSFDLILEQLERVIAVLARRAVEFKHQPIMGRTHGIHAEPTSLGLKFALWHQE
                     MLRNRRRLLAAKETVAVGKISGAVGTYAHTGPAIERYVCEKLGLRPAPIATQVLQRDR
                     HAEALAAVAVCGATIEKIATEIRNLQRTDIYELEEPFRKGQKGSSAMPHKRNPIGCEN
                     LTGLARVLRGNLVVALENVALWHERDISHSSAERIILPDSLTLLHYMLHRLAGILEGA
                     QAKPDRMAENLNKTGGLIYSQRILLALIDKGISREDAYLIVQRNAMRTWEDRVPLIEH
                     LLADAEVAARFTREELERLMNPNDFLKYVDEIYRQCGLE"
     gene            complement(25918..28065)
                     /gene="lpxK"
                     /locus_tag="PLS90_00400"
     CDS             complement(25918..28065)
                     /gene="lpxK"
                     /locus_tag="PLS90_00400"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPK00893.1"
                     /translation="MSRVIVMLPTYNEAENIEPLLDALLALEPRVEILVVDDDSPDKT
                     WLLVQRRAAEEPRLHLLYRKTDRGRGKAGVAGMRKALELGADAVIEMDADWSHDPRYI
                     PRMIAAAERTGAAIVIGSRRVDGGGEAGRHPLRAIITWAANAYIRLLLEFPVRDTTTG
                     YRYYTRACLEAMPWEAIRAEGPEILQELMLAADARGFRMVEIPIRFVDRRAGRSNFNL
                     RIMLRSLGSIVRLWLRPGALVPEGSDLARRRRQRRRRRLRAAGRGVRRFAGRLAAAVR
                     PAAGSRAAGAGADPRDSSPPAPAATVPIRPLAFPWPRRRKAQDELIRVDARVLQRRRL
                     IDAPRLRAGLDTLEPAARWLARPAGYLYGLGRSVHRAARIWRPGGRKALPVPVICVGN
                     LTVGGTGKTPFVRLMAATLSAMGRRPAIVSRGYGARVHLNGPVVVSDGTRVYSTPSEA
                     GDEPRLLAEQCSGVPVIIHPDRAAAGRLAIQQFDPDVILLDDGFQHDRLARDLDLVLW
                     DLRDDPDRTPLLPAGRLRERPGALRRADAIILTHAEYLAPEEREGRRQQTVAALKRHA
                     PAVPIFEAETVVAGFRRLSGRVGPHAEPPAEADGPWPWTGRPVILVSALARPQGLESL
                     ARQTGGRVVRHFAYFDHHEYGPADVDAWRRLRDEEQQAGREAPLILTTAKDGVKLQHL
                     PMFGLPVIVVEIEMRVLDAPRWNAFLHARLNPH"
     gene            28458..29660
                     /locus_tag="PLS90_00405"
     CDS             28458..29660
                     /locus_tag="PLS90_00405"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HPK00894.1"
                     /translation="MIVGVPRETLADETRVALTPAVLPMLAKAGLSVALEAGAGDRAG
                     FPDAAWEAAGARVVAGRDELFREADIIAQVLALGANPEAGRADLARMRPGQLIVGMHD
                     PLGRPEAARELAARGVTALALELMPRITRAQPMDVLSSMATVAGYKAVLLAAAALPRM
                     FPMLTTAAGTIKPARVFVIGAGVAGLQAIATARRLGAVVRGYDVRPAAREQVESLGAV
                     FVELPLTTADAEGQGGYARAMDEDFYRRQRELMAKEVTASDVVVTTAAIPGKPAPRLI
                     TREMVEQMAPGSVIVDLAAPGGGNCELTRPGEEIAHQGVRIIGPLNLPATVPHDASMM
                     YGRNVATCLAHLVRQGELKLDDDDPIVRETIIARDGRVVHPRVLEALGEAPPAQPARQ
                     TPAAPKEG"
     gene            29665..29946
                     /locus_tag="PLS90_00410"
     CDS             29665..29946
                     /locus_tag="PLS90_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPK00895.1"
                     /translation="MEGFIAALTIFVLAIFVGFEVITKVPPTLHTPLMSGSNAISGIT
                     LIGALIAAGMGHTWLTTLLGVLAIILATINVVGGFLVTHRMLAMFRRKR"
     gene            29949..31337
                     /locus_tag="PLS90_00415"
     CDS             29949..31337
                     /locus_tag="PLS90_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPK00896.1"
                     /translation="MSATWQNLAYLIAAVLFILGLKGLAHPRSAPRGNLLGALGMLLA
                     IVATLAGGGIIGWGWIFVGLAIGGVIGLVLALKVQMTAMPQMVALLNGFGGAASFLVA
                     ATALALQAEVPAGFTLQFTIATAASGVVGSVTFWGSLVAFWKLQGLPGENWQGFPGVQ
                     LVNIVLVLLCVALGWIIVAQPPAIGVYWLLVLVAAVLGVLLVVPIGGADMPVVIALLN
                     SYSGVAAAATGFVLSNSVLIISGSLVGASGLILTAIMCKAMNRSLPNVLFAKFGGESS
                     APKADDIYEGKVKATTAEELAMMLEDARRVVFIPGYGMAVAQAQYAVRDLANLLESRG
                     INVEYAIHPVAGRMPGHMNVLLAEANVPYEKLREMDDINPEMAQVDVAIVLGANDVVN
                     PLARADGNSPIAGMPIIDADKARQVVVVKRSLSPGYAGIPNPLFAADNTVMFFNDGNK
                     AVQEIIAAVKAT"
     gene            complement(31407..31483)
                     /locus_tag="PLS90_00420"
     tRNA            complement(31407..31483)
                     /locus_tag="PLS90_00420"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31447..31449),aa:Arg,seq:ccg)
     gene            complement(31609..31794)
                     /locus_tag="PLS90_00425"
     CDS             complement(31609..31794)
                     /locus_tag="PLS90_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016948965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HPK00897.1"
                     /translation="MQIDAEFLCAWCGEPNPTTVDASAGARQAYTEDCQVCCRPNRLT
                     VTLSADGDEAWIDADQE"
     gene            32067..33662
                     /gene="serA"
                     /locus_tag="PLS90_00430"
     CDS             32067..33662
                     /gene="serA"
                     /locus_tag="PLS90_00430"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPK00898.1"
                     /translation="MAMRILISDKMSSEGLANFEGHADFETVYKPDITMDALAEEIGQ
                     YDALVIRSRTRVTRETLARPGKLKIIGRAGAGVDNVDIDAATERGIIVMNTPGANTIS
                     TAEHTIAMMMTLARRTAYADRTMKEGQWAKKDIVGVELSGKTLGVIGLGKIGRVVAER
                     MRAFEMRVLTFDPFLTHDQAHKLGIEPADVDTICREADFITIHCPLNNETRNLINAER
                     LALMKPTALLINCARGGIVNEDDLYEALAAGRIAGAALDVFTQEPLPADHKLRTLPNI
                     VLTPHLAASTNEAQEKVSRDIAVQIREALAGGVVRNAVNAPTVDPKTYEAIGPAMDLC
                     LRIGRFCSQYAQAPVKELDITYSGAKAKHPPTPLTTELVRGFLQYSFSEPVNAVNALH
                     IARQLGIRIIETRIDESDDVYAGLVTVRTTDEKGEVNTLSGALNHARQPRFVIFNEKR
                     IDAIPEGHMLIIENVDVPGIIGSVGTCLGEHKINIASMNWGRVAPGGDALTVINTDQP
                     IPNHVLECLRALPNVLGARHVVI"
     gene            complement(33791..37483)
                     /locus_tag="PLS90_00435"
     CDS             complement(33791..37483)
                     /locus_tag="PLS90_00435"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPK00899.1"
                     /translation="MADAPAPRPDHAATLTALDESGPDSEQPAGAAAPSDESPDGLPL
                     KPYPERAIPALLSPESENTLLTARRSSFVYVLILGSGACGLIYEIVWTRMLTLVFGVS
                     VYAITSVLCAFMLGLALGSWVLGRLADRVSRPLRLYGALEITIGATALAFPWLIDQIE
                     PLFVWINRQIGEDFLLFSIARFAIILPLMLLPTFFMGGTLPVVYRFFARGGLAVAREL
                     SFLYAANTFGAVLGTFLAGFILVEVLGMQGTILMAAAINLLIGAGAILLDRRPSRPTP
                     QSEPRQPSLHETGTHVAAWDLSAGRRQLVLGAIGLSGFCSMGMQVLWNRMLVFHVHNS
                     TYAFSAILMVFLIGLSGGSWLLGWIAPRLRRPLTLFAVLEGLIGLWCILTLLLNGKLT
                     WFSRLLAGELPWDNWYEALALIFIQSAALLLIPTTLMGMTLPLATRLIMVGAESAGRK
                     VGNAYAANTVGAVAGAFAIGFILLSTIGVRESAILLGGLLAAVSAVLLLAGHPPRSGW
                     RVCAAAGCVLLVIVAAVGIPRDVLMRVFAERLGKVVFYKEEVTDTLMVVDMYDSTGTP
                     RPEQRWLVFNDGRGSAGHLTRQDNAFNGHLPMLLHADPKVAAVICIGAANTIAAMAAH
                     KSLERLDAVDLSAGIAEASTWFESNEGILAPPRDPRIHLHVDDGRSFLLGSRYDYDVI
                     QIEPPELHSAGVVFLYTEEFYRIARSRLKPGGILSMWINAFMLGREELRQTITAFHRV
                     FPNGAVWQTPVFGHFIFLGTEEPLHVDLAGMYERIQGDPRLAENLERNNLRDPASLLA
                     LLLMRPETIARYCRGAQTITDDRTRIDFTNPRSALSGYGLIHLHSAIGFDIFYQRSDA
                     VTRPLRTGGWAEISNLVRNADSILPLIDWTGLSEEERDRATARIDAVMDLRRAVASEF
                     AEGMIPNSPFAIEHHWLGSMLAPDPDPDVVYTLTPRLRGEFAGAPFELNELGLRDEEL
                     ESLKRKEFRRILVVGDEVTFGAGVEAADGYVARTERMLNQSVGADARPVQLINAGIPG
                     YTGIQKAALAGRLARELDVEGVIIQHRLFDEAPIPLLAHSDFLTRRDLFGAVLSDVFT
                     TYLGTPAGLRRGLDPLPFSTGVGGQPSSDRQRADLGDLDRMAQAYAELKAECAAADVG
                     LAIFVAPDGQHGGGERDPALNRLADLGLPVWDAAEMPDDSAGDPPSPNTTRSRDRSED
                     PCLDIEGHARLAESLAPPMRSWLEAL"
     gene            37876..39818
                     /gene="dxs"
                     /locus_tag="PLS90_00440"
                     /pseudo
     CDS             37876..39818
                     /gene="dxs"
                     /locus_tag="PLS90_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            complement(39831..41087)
                     /locus_tag="PLS90_00445"
     CDS             complement(39831..41087)
                     /locus_tag="PLS90_00445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPK00900.1"
                     /translation="MPHDSTQLLLVTDSDPLARNVEAGLRRVNAGRRLGVCRRRELAQ
                     HVHDKLQAILIDPGERIAGAETYGTAGVPAGIPVVRFGYLADVLASAADRTRGPFDWL
                     PREFYLAGDPAAVDPRPEDLLLARAIRHAIERHKVDQLLHRSVERLHQTERNFRNLLE
                     RSADAMLVIGEDRRVLFANAAAGQLFKRDAVQLIGQPFEHPLEANRSLEFHTRRHDGV
                     EMVLEVVAAPTSWDEQPAWLATLRETTARTLAVSMLLHEASRDALTGLWNRRILLERI
                     EQEIGRAERGGHALSVAILDLDHFKRVNDTWGHGTGDDVLQTMARILERCCRKADIAA
                     RWGGEEFALLMPETAREGALIVAERLRTETARTPMKTRKGEGFRMTASLGVAQWRAGE
                     DAAALMARADTQLYRAKESGRNLVCA"
     gene            complement(41153..42919)
                     /locus_tag="PLS90_00450"
     CDS             complement(41153..42919)
                     /locus_tag="PLS90_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HPK00901.1"
                     /translation="MQSRRLIRFGAAWLLAAWVIPGVMAAPPPERLHYQGRLTNSGGA
                     PITTAQTLYFSLWRDGSAGGATTANSGTEFYRESATVTPDGNGIFEHQVGSGTPEGGL
                     GSLDPAKFNTPEPVYLQVAVGTLGNVLLPRTRIVSVGYAFVAGTALSAPFAGAAQVTS
                     VTDVTGGLTVVGDLLRVSGSGLSDAVVLVGGKRARVKSQSASEIVCQVPAGIPRGLNA
                     ITVVEDDDLAPSHTGHYVYVTRLVVGVIATTSGADRICVINPETGALLANIDPSPDID
                     TDDGPIQFDFAHEGALALVPVAGNSSTSIVGIDLTLPTPSVSDTADYGSNILSVAVSP
                     DYEAIVLSDFGASNLPRITLNEDFPPYSGSLLGGSSNLGEPSGGNTISPRGATWIGNS
                     LLLVLSTNRDSILGYRRRPGTTDFDDDNAELVLNETPIGTDPLTIRSTPDQSRVVVTF
                     DDGDLAAFYVTPNGVTAVVTPPAAIGGADALHAAIAPDGRTVMVGNAAAAPDGEIRVF
                     DLTGDNLTLTGRFIYADYENEDDGVIQSIAIDPVDGDLAAVTLDDGRIRIYERVGGSL
                     GFLRQVNLGINNTSTLDIRFQP"
     gene            complement(42997..43443)
                     /locus_tag="PLS90_00455"
     CDS             complement(42997..43443)
                     /locus_tag="PLS90_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00902.1"
                     /translation="MRCLFHRTSKRTGPGARSGWRLPAVALALAVALVPTRAAADAAT
                     IATMTGGGAGHTGGGVRLHGALFGYGLPGDHAGRSASASHRIGVGFYPPLAAQIGSNA
                     ADPAWLELTRRGNRVWTEPAAPRQPLRSAEAIVLVRLDPDRPAAVP"
     gene            43694..44896
                     /locus_tag="PLS90_00460"
     CDS             43694..44896
                     /locus_tag="PLS90_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPK00903.1"
                     /translation="MPQANADHPCPDADLWILAGQSNMQGCGQFTPERAAQPDMAEHP
                     RIFAFDSLYEWVVAREPLHHLYAAVDPVHVNLMRQSPYTDLTTLDDLVAGGRASTARA
                     AGPGMFFARHLLAHLPGDRKIALLPVAHGGTTLAQWSPDLPGAPGTTLYSAMLKRLEA
                     SGLRPRGVLWYQGESDAAEDSSASTYAARFKAFVERLRRDTGVPDLPILTVQIGRVAA
                     WERADCWERVREEQRLAAREIPHCYLTHAIEGELCDPIHLSVEAQSVLGRRLANIALT
                     EVYRVGDFGRGIELDSVQVERDPLGGRFLIRYQGVTGALRAVGRPSGFFVRSTQAGLT
                     SIAPWRVELNAAGPGTVLVHAPIGSPEEQAAIGYGEGLDPLCNIFDEADMPLPCFGPL
                     SFDCPPAL"
     gene            44995..45426
                     /locus_tag="PLS90_00465"
     CDS             44995..45426
                     /locus_tag="PLS90_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00904.1"
                     /translation="MTSCVSRAIRRMLLLACLGGVLTAVACTRVHSGAPIYSATVSAQ
                     YDEEGKVGLTRRQALQSAVREARNHILNKALELRLSNGQVLGDLAVVDPFVRAVLEDT
                     VRAARITDRAYSEDGLVTVTVSMELEPLYKLIEEYPQHALQ"
     gene            complement(45462..47144)
                     /locus_tag="PLS90_00470"
     CDS             complement(45462..47144)
                     /locus_tag="PLS90_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00905.1"
                     /translation="MNDGSLDAPGGPATRRAAALSALLIFVAALAAFEPALFNSFAHD
                     DAEQVGAWPLPAGAGDWLRVPLQPWWPLDRLKHEWRPLTRLTILAQKAAAGETTWPYY
                     ALNLLLHGAVSVLLLGAARACGMPRRAALAGALIFAAHPIHAEAVHQVVGRAETLAAG
                     WMLLGLWLHARRPGSAHALWQQPLCYALALGSKDHAILYPVYLGLAAAARGWGGTHPA
                     GGSRGIIGRRRALLTLALLAVGGTYLVLRARITGGLAAAPGEIPFHENPLARATFAAR
                     WPAVLGIFGYAASRLVYPLGLSPDYSAYSLPIERGWGWGWTWIGLLILAGLVGGALRC
                     ARQGSGRGAAWLVLGGLAAWGLTSNAAFPIGVITAERLWYWPSAAGCLGLGWLAAAGL
                     GRAERRLPRLFGYGLALIVVLLCAASWHYAGAWRSQAAYAEWTVARFPASWRGHVNLA
                     REAYQTREFERGAAEARIATRLLPGEEMGWVWLGLNATFLPARRAEAEAALRRALELK
                     PALASVHRNLGNLYQLQGREAEAAEQFRIYLDSPEAADREAIQERLRSLEGG"
     gene            47267..48667
                     /locus_tag="PLS90_00475"
     CDS             47267..48667
                     /locus_tag="PLS90_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPK00906.1"
                     /translation="MNTKQMAKLGIPFGPPMREAVHAVAAAAQAGLKKKSDLAAAVAA
                     VVARPEDYAADGLFGALARELLAARAAPPPFNDRPEPAPWRQWGTDLDPLSIGQMQQA
                     CRLPVAVAGALMPDAHVGYGLPIGGVLATRNAVIPYAVGVDIACRMKLTVLDLPPAAL
                     DGQTDRLKNALLAETKFGVGAAFRDRRQHPVMDEDWSFTRLARELKDKAWAQLGTSGS
                     GNHFVEFGILTLPQADLGLDAGTYLALLSHSGSRGAGAQFATHYSRRAMELHPELPGE
                     LRHLAWLDLDREEGQEYWRAMELMGRYAAANHELIHRRVARHLGARVLLDLENHHNFA
                     WRERHGEADVIVHRKGATPAGAGVLGIIPGSMAAPGYIVRGRGAAEALNSAAHGAGRR
                     MSRKQAVQSFTWSDVRRYLAERNVTLLSAGLDEVPMAYKDIDAVMAAQRDLVTPVARF
                     DPRLVRMAPAGERPED"
     gene            complement(48748..49557)
                     /locus_tag="PLS90_00480"
     CDS             complement(48748..49557)
                     /locus_tag="PLS90_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPK00907.1"
                     /translation="MGKRAARVMDVMAAVERIAPAALAAEWDNVGLQVGRRRDPVRRV
                     MTCLELTPPTLSEARRLKADVIVAHHPLLFKPLKALELDSPSGLLVAGLVRSGMALIA
                     AHTNLDAAAWSTNHALAERLALEVERPLFPPASGEVGGLGLVARPRRPVRFATFIEHV
                     RKSLNLRFVRVSGPERDRVERLALCTGAGGSLLGAAAGQAEIFLTGEIKYHEAIEAHA
                     RGLTVIEVGHFESEVLIARPLAERLARDPVLAAAGVRCHAAGEDLQPFRYA"
     gene            49837..50073
                     /locus_tag="PLS90_00485"
     CDS             49837..50073
                     /locus_tag="PLS90_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00908.1"
                     /translation="MGYHDGEAQYPLEVFLISGDAEHEAFLEADMSMNTEGRGTPVHD
                     LAGLESVHDDLRARTLVRLQYDRFSPGIARRRGH"
     gene            50241..50873
                     /locus_tag="PLS90_00490"
     CDS             50241..50873
                     /locus_tag="PLS90_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK00909.1"
                     /translation="MKRRAVILLAIAGVVLAGGWWFARPGRVRNSPPPPGPIVAFGDS
                     LTAGTGAPRGTSYPDELARLIGRPVLNLGVPGETIADAARRVDDQLLALRPGIVIVLL
                     GGNDVLRRQNLDESFDELERLVRRVQEAGAMVVVVGLRGVTPVGGLGARYRRLARQTQ
                     ALYVPNILGGVFSKRELMADGVHPNAAGYRVIAQRIADALRPHLGDFNEP"
     gene            complement(51073..53229)
                     /gene="glgP"
                     /locus_tag="PLS90_00495"
     CDS             complement(51073..53229)
                     /gene="glgP"
                     /locus_tag="PLS90_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPK00910.1"
                     /translation="MTPVANPSLETHETALRLAQGIRKLAYNIWWTWNPRAQDLFKAL
                     SEEAWITSNHNAIAVIKALSTDELVATLYSKKIQTLARRVIDEFESYLEDENTWGAKH
                     CPGFEQHPVAYFSAEFGMHESLPVYSGGLGILSGDHIKSASDLGIPFIGITLFYRNGY
                     FQQHINGEGWQEESYPALDPHHLPVELVTDANGQPVVCKIRLTQSDITYHAWRLQVGR
                     CTLYMLDTLRPENDLHWREITARVYGGDQTTRVCQELMMGVGGVRLLRALGIDPGVYH
                     MNEGHSAFLLLELMREHVKAGVPLEKARELVREKAVFTTHTPVPAGHDRFSADLLHHL
                     IPHWPEDLGLTMEQFMDLGRVRPGDTHEPFCMTVLALRHARHANAVSELNGRVSREMW
                     RELYGATRQEDVPIGHITNAVHVQGWMNRVTYEFWEQNLGAEWRKHLKQADFWEKVAD
                     PRVLSDEAIWSLRFRLKRQMIEAIRVRLRDQMLRIGTPVSRHYQHVLMPDALTIGFSR
                     RFATYKRATLLFGDIDRAAALLNDPRRPVQVIFAGKAHPRDDNGKQLIQKLWQIANDP
                     RFLGKVIFLENYDIQVARYLISGCDVWLNNPRRPLEASGTSGMKIAVHGGLNLSILDG
                     WWREAYDGTNGFAIGEDRHAPDVEQQDAEDMQNLYRVLEQELIPMYYDRDGMKIPRQW
                     IRRIRRAMVTLIPRFNTDRMVAEYAEKYYGAIPSEE"
     gene            53652..55727
                     /locus_tag="PLS90_00500"
     CDS             53652..55727
                     /locus_tag="PLS90_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK00911.1"
                     /translation="MIVRFFKRNSILIGFSLIYLLLVVLAMVELDRQYYQQRKRTYIT
                     ERYLVTSINQEIGGENLGDLEQVALEALRTNNPQAMQRLREQIWKILRADNFVYRIAI
                     RPGDDQEVEGQPLNFIVDLPSQKEIEEKIAAHNSFANSLFLRDFSGHLSTPIVAGGRQ
                     FGRLYAFYTSPIGNPEIEEITRTYRWIALWIWLLITVLAVGLARSLLLPLRNVMSSLE
                     ASTPQRTHFIPRPRTRLESLYNRMALDAVIARMQGQLREEIARRPQMTGWEVVHFIVE
                     AFRDQVGLALVACLEMVAEGPGAIRATGQRVIAGPRDWAKHQPELVQLIDDAMPRDGR
                     IQASLLLDEIGLRGEGLVHLVADPERTGIRYLLALALERHLDHAAHGSLQNMLERLTE
                     LIEAGLQTLTLRNRLLVQERGRANISLSRNLGHDLTNIIATSKLELMALDRLLKGGQL
                     PDDDRRRQILVESLHGLLRSVKFMQETVNLYRSYAFLQHPVLEVQDGNQLIGESMELF
                     EISISAKVRLEQELAPDAPRCVVDPRLIKLALFNLFSNALEAIRKKDPERTAAGCIRV
                     VTRRSAEGGLCVAVEDTGTGILNERNEPAQPHEIEKIFELGYTSFRLGGSHGEGLGLN
                     WVRTIVQDLHGGSIFAENIEGAGARFVIVFPPVEQAPDLENTEMHAARYMRQIQGLVE
                     EQEQSARHG"
     gene            complement(55718..>57248)
                     /locus_tag="PLS90_00505"
     CDS             complement(55718..>57248)
                     /locus_tag="PLS90_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00912.1"
                     /translation="AVVAVLFGLALALGEFSPLFAAFYRFVPGFDRFRVPARLMLFVV
                     GGQALAAALALDAMLRVGSPAAIRRRLAGGAAVALALVWLSFALRDQVWASFPESPVA
                     LGLGQPGVDEAYAAETRHAGWMGLRVSVALAAALALLWRVAGRRDAASAPRRLGPALG
                     LPALAALELALLALPYLKDVYFTRYEATYYPRTPLVQKLTELDQGGRILWLDNVHAWM
                     FDQNQPEVFPNRLLMYGLDEARGYDPVNARWIGVWSNLLAGFPPEQNPRGFMLIPVIA
                     RPAWLTLMGVDTILTYGAGAAIDLSGTPGLELADRLEFPEGDLELWRNTWFRGMAFAA
                     PMPRLVNQRDEEDWITPLVHSAGLATPAADPATTATAGIDPMATIVTTPGVLWLGERL
                     TPEIDASYRVEPLDDPAEAAPNRFGYRVDFPRPALLCLAQSAWPGWGVRLDGAPAPVQ
                     RLSGVFLVTAVPAGSHEVIFEFLPPGLLPGRWISILAAVILAYGLIRSRLKGSRPHGQ
                     P"
BASE COUNT         9269 a        20003 c        18887 g         8989 t
ORIGIN      
        1 cgggatcgtc cgtcgcctac cggcagcact tcttattgat ggcgttctgg tagatcagat
       61 cgaccagttc catgctctta accgaatcgc gcagatcatt gtgcagcggc ttgcccgagg
      121 ccacggcgtc gatgaacgcg cggctctggg cgaaaaagcc ctggaacaca tggaattcct
      181 gcgattgcgc cgcctcggtg ttggtcagct tgagcgtcgg ctccgcgccg ttgtcggtcc
      241 agaccacgcc ttccgcatcg atgtccagat aggccgaggc gtcgggcgcg tgcagctcga
      301 acttgaaaaa tcggcggccc gtgcgccagt tggcctgcag gatgccgacg cagtcattct
      361 cgaaatagac cagcgaattg aagctgacgg cataccagct atcgatcgtc cgcacttcgg
      421 aagcgaccgc cttgacttcg gaaaggcccg cgtagtaacg cagcgagtcg accgcatgga
      481 tcgcatcgca atgcaggata tcgatcgcgc cgccgtagta cgggtgtttg tcctgcggcg
      541 ggaggtcctt gtagaaacag gagttaacct gatgcaccgg accctttttc ttcacttcct
      601 cccagcagcg ccggacgagc gggtgatagc gccgctggaa gccgacgccc gagatcagct
      661 ttttcttctc ggccatccgc gccaggcagt cggcctggaa cgtcgtgacc gcgggcggtt
      721 tctcgatgaa caggtggtgt ccgcgctcga gcgcctccat cgcgatgtcg aacagatgat
      781 gggggggcat caggacgtaa accgccgccg gcttcgtctc gtcgagcatc ttgcgccaat
      841 cggtgaacgt cttgccgatc ttgaacttgg cggccgtttc cttcagtcgt tgctcattca
      901 agtcgcacaa acctgtgatt tcgacgtcat ccatcgtcgt gagcgacgga taatggacgg
      961 tgttcgccat cccccccgcg ccaatcaacg caaccttgac cttgctcatc gcctcaattc
     1021 ctcctgcggt tcagcttgac tggcagccaa gataaaggcg gggcgcggaa aaatccagcg
     1081 cgaagtatat gcttggttca ctctctatcc cgttctggtt accaggcctg catgccgttg
     1141 ggcgaacagc cgcgcgaacc cggcgcggca ggggaccggc gccctcgccg gtcccggtct
     1201 gaaaccggcc cattcaggcg tgcaacgccg aacctccttg acgcgccgcc gcgcgatccg
     1261 ctatcaaagg tgacgccagg taaccgaaag gtgaaaccag gggaaacgcg gcggcggtcc
     1321 gaggccggac gcccgtgccg ccaacctccg tccccatcat ccgggagaaa gctcaatgaa
     1381 acgagatcgg cgcgcggcgg acttccgccg cctggtgacg ctgggcgaaa gcacgaccgc
     1441 cggcgggtgg gccagccatc gcggccgctg ctgggccagc ctgctcgagc agaaaatcaa
     1501 cctgttccag acgctccccg tgcaactgtt caactccgga ctgggcgcca atctggtcac
     1561 gcgcgaagcg cccgcctacc cgcacagcgg ccggcccgcc gccgacgagc gtctcgagcg
     1621 cgacgtgatc cggcatgaac ccgatctgct catcctggcc tacggattga acgatgcccg
     1681 gggcggcacg tcgctgccgc tgttcatcgc aacgctcgcg tcgctgatca accgcgttcg
     1741 cgagcggctc gaccccctga tcgtaatcgt cggctcgttc tatgcctgcc ggttccgcta
     1801 cgatgatccc aactgggagc atgccgacct gatcggcttg cggcaattca gcgacgcgtc
     1861 gcgcggcgtc gccgaggacc acgactgtct gttcgtcgag atgatttccg ccttcgatgg
     1921 cgccgactgg ctgatgcact acgacggcgt ccatgccaat gacctcggcc accaggtcat
     1981 tgccgatcgc attttcggcg tgctggccgc caactgcacg tgcctcgcga cccggacgaa
     2041 ggccttggaa ccgcagatcg ccccctggcg cgacgaatcg acgctgcgga cgccgatcct
     2101 tccgcacgcc tgacccctac gtgatcgagt cgagatgtcc agccgtcgga agggacataa
     2161 gggacaaaca caaggaacaa acgggaggtg tggagactgc attcatgtcg cccagggcgt
     2221 cgtgaaggga gactcaggaa aaacgctcag atcctccgct gcatgcgctc tcggcgcgcg
     2281 gcgggtcagc cctgcgccac gtcggtctcg ccgccgtcaa cgtccttcat cttgccaagg
     2341 aattgcggca gttcgacgcc ggccatgcgc gcgacttcct gcagcggggg caggctgccg
     2401 accaggctgg agacaaaccc cgccgtgctg gaaccgccgc gcccgttgcc gccgcccgaa
     2461 tcccacaccg tgatcttgtc gatcttcagc ttgctgatcg cctcaacctg ccgcgcgacg
     2521 atctcctcca gcttttcgat catcagcagc gtcgcggcgg cgttcgcatc gccgccgcag
     2581 ctcttgacca gcgccgcgta gccctcggcc ttggcttgga gcacctggcg cgtaccggcc
     2641 gcctccgcct ggtagcgggc caggatggcg tcggcgtcgc cgcgcgcctc gcgccggcgt
     2701 ttttcggcct cggcttcggc cgcgatctcg atcttgcgcc gctcgatttc ctggcgcacc
     2761 acctcttcgg ccgtcagccg ctcctgctcg gcgcgggcct gcgccttctg gatctcgact
     2821 tcggcctcgc ggcgcgccac ttcgccgcgt cgcgtcgcct cggcctgctt gatcgccagt
     2881 tcggcgttgt aggccgcgat gttcgccttg gcggtgttct cgcccttgac ggcatcggcc
     2941 tcctgctgct ggacgaaaac gcggcggtcg gcctcggccg ccttgcgccc cttatccgcc
     3001 gcggcgatgt tttccgccac gcggatttcc ttctcgcggt tggcctcggc ctcgccgatc
     3061 gtcgccgtcg tctcctgctg ctggacgaag acgcgctgat cggcctcggc cgccttgcgc
     3121 cccttgtccg cctcggccac gttctgcgcc acgcggatct cgcgctcgcg caccgcctcg
     3181 gcttcgccga tcgagccgat cttgtcctgc accgcgacgt cgatcttcgc ctggttgatc
     3241 gcctcggccg ccgccttctt gccgatgctt tcgatatagg tcgattcgtc ggtaatgtcg
     3301 gtgatgttga cgttgatcag atagagcccg atcttgttca gctccggctc cacgttcttg
     3361 cgaatcgctt cgaggaaact ttcccgatcc tggttgatct gctctatcgt cagcgaggcg
     3421 acggtcaggc gcaactggcc gaagatgatc tcccgcgcca tgtcctcgat ttcctgccgg
     3481 cgcagcgtca gcaggcgttc cgccgcgttg ttcatgatct cgggctgtgt gctgacgccg
     3541 accgtgaacg tgctcggcac gtcgatccgg atgttctgca gcgatagcgc gttcttcagc
     3601 gggatgctga tcgtcatcgg caccaggctc agatacatgt aatcctggat cagcggccag
     3661 acgaatgtgc cgccgccgtg gatgcagcgc gccgattgcc ccgtgccgac cttgccgaaa
     3721 atcaccagga tccggtcgga cggacagcgc cggtaacgcg aagccaggaa cgcgatcgtc
     3781 gagaacagca gcacgatcac gatcacgatc agcacgaccc agaaccccag gctggagtca
     3841 acgaatggca tcttgaattc cccctgcgaa ggtttgatcg aaccagaaag tcaggcgcgc
     3901 ttgaccagca tcaggccgct gttgacgacc tcgacgactt cgacgcgctc gccggttttg
     3961 atcggctcat cttccttgct catcgcgtcg acgatccggc tgcgcccctg gatgctgatc
     4021 tggatctggc cggtccccct gggcggaatc gtcaggtaga ccagggccga ctggccggcg
     4081 gcctggcgca tgtcgatatt gccgctcgag tgcagcccct gcatcatctg ggtcagcttg
     4141 gccagcagca ggaccatcgc ggcgccggcc accagcgcgc cgaggatcga ggccagttcg
     4201 ccgacgcggc tgccgcggtg cagcgccagg cccgtcaagc cgaagatcag aaaaaagccg
     4261 gtgatcccct gcagcgtcag caccttgaag ctcaggtccg ccccttcggt cagatcggcc
     4321 atggaaaggt cgacgccgac gtcggcgccg tggtcgccaa ggccgaacag gcccagcacc
     4381 ccccgcgcaa tgaaaagcag cccgccgatc agggtgcaga tcagaaagac ccgatcgacg
     4441 ccgctcaggc tgatccagaa caggtccacc atgggatcgg ggacaccttc ctttcaagca
     4501 accggcaagc ggccaataca acgcggacaa gatcgtctgg aaccatcatc ggcgggcggg
     4561 cgggggagtc aagccgaatc catggccggc cccagcggac ggcgggcaag ctgcgcgccg
     4621 ttgcggggaa agcggctggg agtgggaccg cgcttttgat aaatcaggac ccggcaggct
     4681 tttccgtccg agcggcggta gtcgagttct ccggccgcgc gggcgccgag acgttcggga
     4741 acccccgcgg ccgctgcgag ttccatcgtc tgatcggccg acttgatggc ggcgaacgcg
     4801 cctcccacgg ccgcgaacgg cagcgccagc tcgagcagca cgggcagcgg cgcgacggcg
     4861 cgcgccgtcg ccaggtcgta tgcctcgcgg tgccgcgcgt cgtgggccag ttcctcggcg
     4921 cggccggtca gaacgtcgac gcaggggagg ttgagttcgc gggcggcgcg ctcgagaaac
     4981 gccgtccgct tccggcgtga atcgagcagc acgaccttcg ccgacggcca gagcagcgac
     5041 cagacgagcc cggggatgcc gccgccggcg ccgatatcca ggatgcgggg agcggccagc
     5101 gccgcgacgc gcgcgtgcag cgccgccgcc gcccgcacgg catcctcgat atgccgcggc
     5161 cagaagagct cgggtgtctt gtccgcggta aggttctggc gggtgttctc cgcaaggatc
     5221 agttcgacga gagtggccaa gcggtcgagc gggtgggccc gcgtggggcg agccggcgcg
     5281 aatggttcca gaagcggggc aatggttgac tgaggggtgg tcatgaagaa accgggctca
     5341 agcgaccgtt ccaccccgag gatcgaccgg cggctgatcc tgaaagccgt cggatccgac
     5401 cgatccgacg ggggacgata cgccgcgctt attccactgg atacaactgc agcgagagat
     5461 ccagtgcggg cgccgaatgc gtcagcgcgc cgatcgaaat ccggtcggcg ccggcccggg
     5521 cgtaggccgc gacgtttttc agcgtcacgc cgccggagat ctcgaacggg atgccgcacg
     5581 agcggaagcg cgcgatgacc gcgcgcgccc gcgccggcgt gaaattatcc agcaggatca
     5641 ggtcgacccc ttcggcgcag cagatcgcaa cttcctcggc gtcgcgcgcc tcggccatca
     5701 ccttcagcct tttccgtccg tcttgcgcgc cgggcgcctt caccgcgcgg atcgcctcgc
     5761 gcaagccgcc ggccgcgcgc gcgtggttct ccttgatcag caccatgtcg aacaggccga
     5821 agcggtgatt catgccgccg ccgacgcgga cggcgtagcg ctccagcgcg cgccacagcg
     5881 gcgtcgtctt gcgcgtgtcg cagatgagcg ggccgttggc gccccccgcc gcgtcgtgga
     5941 accggcaggt cagcgtcgca atgcccgaga gccgctgcag aaaattcaat gccagccgtt
     6001 cgcccaccag cagcccgcgc atccggccct caagcgtggc gacgcggtcg cccgcccgca
     6061 cgcgctcgcc gtcgcggcaa tgccattgga ttttcacgcc gcgatcggcc agacgccaga
     6121 cggccgcggc gaccggcgcg ccgcacagca cgccggcctg cttggcgatc agctcgccgc
     6181 gcccgcgcac gcgcggcgcc acggtgcgct cggtcgtcac gtcgcccggg ccgagatcct
     6241 ccttcagcgc cgcgcgcagc aggcgggtca gctccggcgt aagctgaagc tgcggctgcg
     6301 cgcgattgac cgtctcatgg ttcggcgcca tagctgtgct ccgcggtcgg gaatcggccg
     6361 gcgcgcacat cgtcggcata cgcggcgagc gcggcggcgg cctggtcgtg gagggtcgca
     6421 tacgatttga cgaacttcgg cctcggaccg tcgatcatcc cgagcaggtc ggaaaggaca
     6481 agcacctggc cgtcgcagtc gggccccgcg ccgatgccga tcgtcgggat ctggatcgaa
     6541 cgggtgatct cggcggccag cggggcgggg atcccttcca gcacgatgga aaacgcgccg
     6601 gccagctcca ggtcgcgggc gctgtcgcgc agttgccggg cggtctgctg gtcgcgaccc
     6661 tgcacgcggt agccgccgcg ctggtgcacc gattgcggca gcaggccgat gtggcccatg
     6721 accgggatgc cggcggtgac gaggcgctcg accatcggcg cgacttcgct gcccccctcc
     6781 agcttgacgg cctccatgcc gccctcctgg atcatgcggg cgcagttgac aagcgtctgc
     6841 tcggtcgaga cgcggcaggt catgaacggc atgtcgccga caaggaaggc gcgacgcgcg
     6901 gcgcgcgcga ccgcggcggc atgatgcagc atcgcctcca ggcggaccgg gatcgttgtt
     6961 tcgtaaccca gtgcggtgtt gccgagcgaa tcgccgacca ggatcaggtc gatgccggcg
     7021 cgctcgcacc aggcggcggt aatggcgtcg taggccgtgg cgacggcaat ccgccgcgcc
     7081 tcgcgtttca tcgccgcgaa atgcgtgacg cgcagcttga caggatcagc cgggggcggg
     7141 gcgtgcgtgg acacggaacc ttccttgagc tgggtgagca aaggatgcga cgagctgagc
     7201 catcggcgcg gccgatgcgg ccaatgcggt gcgcggcgcg cgattcccac ccgggtgaat
     7261 ccgcggccgc aacgcgccga tcttccattc atggcaccgg cccgggttga tgtccatagg
     7321 attcagattt cctttcccgt gcggacccca ttcaatgggc gtgctcgcgc cgcgcgcagg
     7381 cgatgccgtt gagctgctcg gcgatctcca cttccagggt tgaatgctgg atctgaaacc
     7441 gctcggccag aatcgcgcgc aggtcgcgct tgatgcactc catctcgggc agcgaggcct
     7501 cgccgatcac cacgtgcccc tcgaacgacc ggtggtgctc gtcgatctgc cagacatgca
     7561 cgtgatgcag ctcgcggacc gccgccaccg tctccagcgc cgcggcgatt tcctccgccc
     7621 gcacgtcgga cggcgtcccc tgcatcagga tgtgaatcgt ctccttcagc attccgacgc
     7681 tctgccacag gatgtaggcc gagatcagca gcgtgacggc aagatcggtc cagtaccact
     7741 catacaacag gatcagcgtg ccggcgagca tcacgccgac cgaggcgagc gcgtcggaca
     7801 ggttatggat gaacgccgcc ttgatgttga ggctcgtccg gctcatcgtg taggtcaaca
     7861 gcgccgtggc caggtccaca gccagcgcca ggccggccag ccagacgacg atccagccgt
     7921 cgatcgggtt gcgctcgaac cagcgcagaa ccgcttcgaa cacgaggtag agggcgacga
     7981 gaatcagcgc cgtcaggttg atcagcgccc cgatgatctg cgcccggcgg taaccgaacg
     8041 tgcgcagccg gtcgggcggc cggcgcgaga tccggcgcgc cgccagcgcg atcccgagcg
     8101 aggccgcgtc gttcaggttg tgcagcgcgt cggcgaccag cgccaggctg ccggccagcc
     8161 cgccgccgat cacctgcgcg accgtcaaca gcaggttcac ggcgaccgcc caccacagcc
     8221 gcgcgtcgct catcaagtca gtcgggtgtt catggtattc ccgtgccaaa tccgtccctc
     8281 gtcctcactc ccagatcgtc cctcgtctct cgtcctcgtc ctcggctctc aaggattgct
     8341 cgatcacccc attctacccc cgctgcggcg ggatgctgaa ccacttgtag agcgccggca
     8401 gcacgagcag cgtcaggatc gtcgacgaga ccaggccgcc gatgacgacg gtggccagcg
     8461 ggcgctgcac ctcgctcccc gtgccgctgg agaacagcag cgggatcagg cccagcgccg
     8521 tcgtcgcggc ggtcatcagc acgggacgca gccgcaggca ggccccgcgc acgcacgcct
     8581 cggccatcgg cagcccctcg cgcacaagct ggttcaggca ggtgaccagc accatgccgt
     8641 tttcgagcgc gatgccgaac agcgcgatga acccgaccga cgcgggcacc gagaggttga
     8701 gcccggccag ccagagcccg accacgccgc cgaccagcgc cagcggaata tttaatagaa
     8761 tcagcagcgc attgcgaatg gaattgaagc tgctgaagag cagcaggaac accagcagca
     8821 gcgtgacggg aacgacgatc gcgagccggc ggttggcctg ctgctgcagc tcgaactggc
     8881 cgccccaggt aacaaggtag ccggggggca gatcgacgcg cgcggcgatg gcggcccgcc
     8941 cttgctcgac gaacgagccg atatcgcgcc cgcgcacgtt ggtctggacc gtgatgaagc
     9001 gctggttgtt ttcgcgcgtg atctggcgcg gaccgacgat ctcctcgacg tgcgccacgt
     9061 cttccagcgg gatgcgcagg tcgcccggcc cggggatcag gatgcggcgg atcgcctcga
     9121 tcgaggcgcg gtgctcgggg tgataccaga cggtgatatc aaaccggcgg accccctcga
     9181 aaatctggcc cgcctcggcg ccgccgatcg ccgcgcggat cacctcctgc acgtcgcgca
     9241 cgttgaggcc gtagcgttcg agctggtcgt agcgcatgcg gatgcgcacc tgcggcgttc
     9301 ccgtcacctg gtccttctgg acgtcggcgg cgccggggac ctcgccgatc acggcggcga
     9361 tctcgtcggc cttcgccgcc agcacctcca tgtcggggcc gaacagcttg atcgccagtt
     9421 ccgccttggt cccggtcagc agctcatcga cggcggcggc gatcggctgg gtgaagttga
     9481 actgcgcgcc ggggtagtcc tcgaacgcct cgctcatcat ctgcatcagg gcctcgggcg
     9541 tcgccgcgct ggtccattcg gcgcgcggcc gcagttcaac gaaaatctcg gcgttgttga
     9601 ccggatcggc gtgcgcgccg acctcgccgc ggccgacgcg cgagacgacc tgcctgacct
     9661 cggggaatcg cgcgcgcagg tcctgttcga agcgggtaat cgtccggccg gcctcctcga
     9721 gcgagatcga cggcgccatc gtcgcgcgga tcagcaggtc cccctcgttc agccgcggca
     9781 ggaattccga accgagcaac gggaagatcg ccagcccgag cgcgaagatt cccaccgcca
     9841 gcagcacggc cagcacgcgc ccgcgcacga agaggccgac caccggacga tagggataga
     9901 gcagcagccg gacgatccag ttctcgccgg cgggcttgtg gtcgcggcgg gcgcggcgcg
     9961 gcgggcgcat gaaacaatac gcgaacaccg gcgccagcag catggcgaag agcagcgaac
    10021 cgaacatcgc cagcgagatc gtgtaggcga gtggacgaaa cgtcttgccc tcgaccccct
    10081 gcagcgtaaa gagcggcagg aacaccagca cgatgatgcc aatggcgaag aaaatcggcc
    10141 ggccgacctc ggcgcacgcg cgcgtcacca cgcccagccg cgactcgtcg ggatcgctct
    10201 cgcgcaggac gcggtcaacg ttctcgacca tcacgatcgt gccgtcaacc atcatgccga
    10261 tcgcaatcgc caggccgccg agcgacatca ggttcgccga aatgcccagc agcttcatca
    10321 acatgaacgc aaacaggatc gagaatggaa tcgagagtgc gacgaccagg ctcggccgca
    10381 gcccgcccat gaacgccagt agaatgatga cgaccagcac gatcccctgc accagcgccg
    10441 tcgtgacggt gttgacaacg ttctcgacca gcgtggcctg gtcatagtag gcgatgatgc
    10501 gcacgcccgc ggggaggatt cggttgattt cgtcgagccg cgccttgacg cgcgcgatga
    10561 cctcggaggt attcgtgccg atcaatttga ggaccatgcc gacgaccgct tcgccccggc
    10621 cgtcggccgt cgccagcccc tggcgcggct cgccgccgat cacgacatcg gcgacctggc
    10681 gcagataaac gggcgtgccg tcgaccgtct tgagcacgat tgattccaaa tcgtcgaggt
    10741 cctcggccaa gccgaccgag cgcacgatgt attgctcgga attcttaatg ataaactggg
    10801 cgccgacatt ggaattattc gcctcgaccg attccaccac ttcgtggatc gtgagcccgt
    10861 agcgcagcag gtccgccggc cggatcagaa tctgatactg cttgacctgg ccgcccagcg
    10921 agagaacctc ggtgacgccc ttgaccgtct gcaggttgta tttcacgatc cagtcctgga
    10981 tcgtgcgcat ttcggtcggg ctgtagcggc cggtggcgtc ctccagccgg tagaacagga
    11041 tctggcccat gccggtcgtg atcgggccca tgcgcggctc gccgaagccc tcggggatct
    11101 gctcgcgcgc ttcgccaagc cgttcgccga caagctgccg cgcgaaataa agatcggtcc
    11161 cgtcctcaaa gtaaatattc accaccgaca ggccgaaatt ggagaccgag cggatgtgat
    11221 cgacgcccgg cagaccgttc atcacgctct cgaccggata cgtcacgaat ttctccactt
    11281 cctcgggggc cagcccctcg gtcagcgtga agacctgcac cagcgccggc gtcacgtcgg
    11341 gcagcgcgtc gaccggcagg cgctcatagc tgaaccatcc ccccgcgacg accagcacgc
    11401 cgagcgccag catcagccag cgcgtggcca gaatggtttg tatgtacttc agcatcaact
    11461 cgtttctttc cttattccgc ggtcgatcac gtgaactcag tgcgcgtggc cgtgggcgtc
    11521 gaagcttccc ttcgccagtt ccgccttcag cgtgaacgcg ccggtggcgg cgtattgctg
    11581 accggcgcga agtccctcgc ggatttcaac ttcgtcctga tcctcgcggc cgggcagaac
    11641 gggcgccggc gcgaagccct ccgccccgcg cacgaacacc acctgcgcgt cctccatcgt
    11701 ctggatggcg gtcttgggaa ccacaatcgg cgcggggacg gcgtcgacgg cgacgcgccc
    11761 ggtcacgaac atgccggcgc gccagcgccg cgcgggattg ggcaggacga cgcgcgccag
    11821 cgccgtgcgc gtctgttcgc cgacgatcgg gccgatgtag ccgatctggc cgacggccgg
    11881 cgccgtcgcc tcgccggcgg agatcactac ttgctgcccc tcgcgaatcc gcggcaggtc
    11941 cttctggtag acctccagat cgacccagac ggtcgacaga tccgcgatcg taaacggctg
    12001 gctcgcttcg tcgagcactt caccccgcgt gatgtgtttc tcaatgacga cgccgtcggc
    12061 gggggcgatc aaagtgtaac gggtcagttg cgcgtcgggc cggttggaaa gcgcctggag
    12121 ataatcctcg gaaaagccga gcgcgcggag tttctggccg gcggcgttca gttcaatgct
    12181 cgcctcgcgc accgcctggc gcgcgtcaag atattcctgc tcggggctga gttgcttgtc
    12241 aaagagggct atttcgcgct cgtaggtcag ttcacgcagc gcgacgcgtt cgcgcgctgc
    12301 cagatattcg gccttcagat ccgccagatc gcggctttcc agcacggcca tcacgtcgcc
    12361 ctgccggacc gcgtcgccca gcgtggcgtt cacttcgcgg acgatgccgg ccacgcgcgg
    12421 cgcaacgtga gtcagggcgt cttcgttcag gcggatctcg ccggggaact cagcgtaggc
    12481 ttcgagcgtg ccgctggtcg ccgtcgcgac ctggatgccg agccgctcga gctgctccgg
    12541 cgtgaacgac acggcatgaa ccgcctcatg cgcgccgtgg ccatgcgcat cgtgatcatg
    12601 ctcgtcgtgg ccgtgcgcat cgtgcgcatg ttcctcgtgc gtatgtgcag cctcgtggac
    12661 gtggccggtc tccgccgccg aacgcctctc gtgctcgtgg tccgcatggt cgtgatgcgc
    12721 atcctcctgc gcctgcgcgg aaaagccaat ggaaatgcgc gccagcgtcc ccgagcggat
    12781 gccggcggcg acgccgatgg ccagcatcgc ggcaagcgcc gcaatcaacg ccttgcttct
    12841 catggtttgg tctcctcgat cctgtcgttc gcgcctgcgg aatcggccgc gacggtcgcc
    12901 agcggcgccc cgatcaagcc ctccagttcc gccgcggcct gctgaaattc ggtcagttgg
    12961 tccaggtaac gtccgcgcag ttcaaagacc cgttgctggg cgtcgagcac gttgatcagg
    13021 tcgtattcgc cgagccagaa cccttcgagc agggattcat acaccgcctg cgcgtcggga
    13081 aggaccacgg ttcgcagccg ctcagcctcg tcgcgcgcgc cggccgcgcg cccatgcagc
    13141 tcaatcagtt gctcgcgcag ccggcggcgg gcggccgcct gttcggcctc ggcctgttcc
    13201 aatcggtggc gcgcggcggc gatctggccc tgattgcggt cgaacagcgg caggggaatg
    13261 gaaaaaccta atatcaatgc gtgattttcg gtttcattaa aatagcgggc gccgacgcgg
    13321 agcgtcggat cgggccgcgc ctcggcctcg gccagttcga gcgcggcgcg gcgaaccgcc
    13381 atctcggtcg cccagcgggc aacttcgggg ttgtccgcca gcagcgtctc cagatccgcc
    13441 gcggcgggaa ggggcggcag gcgttccaga tcgccgacgg cctcgccgaa cacaggctcg
    13501 gcgtcccaca gcgcggcgag acgccggcgg gcagcatcca gttcggcttc ggccgccgcc
    13561 aggtcggtgc gctggacttc ccacgccacg cgcgactgct tctcctcgat cggcgaaatc
    13621 ttgccggctt cgacgcgccg cgcgacggtc tcgtgggcct gcgtcgcgat ctccgccaat
    13681 tgccgggcca gattgacctt ctcctgcgcg gcgagcagct cgacgaacgc cgcctgcacc
    13741 cgcgtgagga gctccagacg ccgggcctca atgtcccagg ccgcggcgtc ggcctgccgc
    13801 tcggccagat ggcggcgctt gagccgcttg ccggcccgtt cgatggtctg gctcagcgtg
    13861 agggtggcct cggcgccggt gaaaccgctc aggtcctgct gctccggctc cgcgcccgac
    13921 gtgccgccca ggagatcttc gatctccgct tcaagctccg gattgggtgg caggtcggct
    13981 tgcaatacct cggcctcgcg cgccgcgact tcctgctcca gcgcggccag ctccgggtgc
    14041 cgatccagcg cccggttgat cgcgtcggcc agcgtcaaca cccccgtcgg cgcttcgatc
    14101 ggcagcgtct gatactcggc cgccggttcc ggcaagctca cgcgcgcgaa ggtctcggcg
    14161 ctcccctgcc ctggcgggca cggcgccggc cgactggccg tgtagggtgc aggtgcgagt
    14221 cggaggcatc cggccaccgg cgcgatggcg gccagcaggc ccagccagag aggaagacgc
    14281 atcgtcgagc tccgcaaagg actgaagcgg ccgggcgggg aaccgtggcc gcggctcggc
    14341 gcccggcagc gcgggcgccc gcgattcagc cgacgcgggg tctcaaatga tgaggatgac
    14401 ggttcggagt aattgcaggg caagcggggc gcagcgcggc gcgctggagt cgatggcggt
    14461 cgggccgcgc tgccccgtga acagcccggc ttcgcggcgc ggcatgtcca cggaggccag
    14521 aaccggcggc gccggcgctt cgcagccttt ccgctcgatc ttgaccggga ccggctgggc
    14581 atggccgccg atcaggagca gatccaggca gccctcgtgc gggtccgcgc cggtgatgac
    14641 gcgcgcacgc tcatccgcgg cggcgggacc gggcgcgcaa tgcggcgcgc agaaccgttc
    14701 cggttggtgg gagagctcga cggccagatg atcctcgtcc aggcagagga cgaagccatg
    14761 ggccgcgccg ccgacgccga acagatagag cagcggcagc aaccagatga aacgggcgtg
    14821 gcgcaagcgg gacaacacgg cggatttata gcaaaacgtg acgtttttgt cgagtgatcc
    14881 gcggcggcat cccgcacgat cgccaatcac gcgatcttga tccccatcag actcaagaac
    14941 tcggcgcgcg tctgggggct cgtcttgaac acccccagca tttcgctcgt cgtcgtcagg
    15001 ctgtcctgtt tctccacgcc gcgcatcatc atgcacatgt gatacgcctc catcacgacc
    15061 ccgaccccct tggggctcaa gacctcgtgc aggctctcgg caatctgggc ggtcaggcgt
    15121 tcctggatct gcagccggcg ggcgaacatg tcgacgatgc gcggtatttt cgagatgccg
    15181 acgaccttgc cgtcggggat gtaggcgacg tggcatttgc cgtagaacgg cagcaggtgg
    15241 tgctcgcaga gcgagaaaaa ctggatgctc ttgacggcga ccatctcgtc gtatttctcg
    15301 tcgaagatcg cgccgttgac gatcgtgtgc aggtcggtcc ggtagccctg cgtcagatat
    15361 tcccaggcgc gggcgacgcg ttcgggcgtg cgcgccaacc cctggcgccg gggatcctcg
    15421 ccgatctccg tcagcagcat gcgcgtggcc tcggcgatcc gcgggttggc ctgctcgtcg
    15481 gccaggtctt cgtccagccc cacgcgttcg attggttgcg gctggggatg tttcttgggc
    15541 atggtttggg ggctttccaa gcagtagtgg gcggatgggg tcaaacggaa tgggaggaag
    15601 tcagccgttg ccgcgcgcgg tcgtcagcga ctccatccgg gcgacccctt cgatgccgac
    15661 gatggcatgg acgacttcgt cggccagatc gggtggaaac cagaaaccga gttgggccag
    15721 cgtggcgaaa ctggcgcggg cttcctcgcg cgccagttcg agcaccgtgc gggccgaatc
    15781 ggcgaaatgc tcctcaacca ggccgtcgcc cgcttcgcgc gcctcccaca gccgcgccag
    15841 gcgggcgcgc tccgcgtcgg agccatgatg atggatctga gcgacgagca ggttgtggct
    15901 gcggcgaccc aggtcgaact caaaatcggt caggtcgtcg acaatctgga aggcggtgcc
    15961 gagctgggcg atggcgggtt cggccgccgc cacgcgctcg ccgatcgcgc cgcgttccag
    16021 aatcgccggc gcgacaaacg ccaggccgaa cagcatcccg ccgcggaccc gatgcacgtg
    16081 atcgatcatc tggcgcggcg tggggatctc gctcacgccg gcctcctccg agccctcgag
    16141 caggccgatc tcggccatgc gatcgaggag gcctcgctgg acttcggcga acgcggtggc
    16201 ggagaacagg ccgtcgttga cggcgcggtc gccgacgcgc cgcagcagcc gctcgaagca
    16261 catgagctgc aggatcgagc cgaagcaggc cccttccacc tcgcgcagcg gcagcaggct
    16321 tttgctctgg ccgtcgaaca gattatccgc cgccgtgatc gtccccttga tgcagaaatt
    16381 gagccgcgcg taaaacggga aacgatccgg cgagatgccc agcgactcaa acagcgacag
    16441 aaagagcgtt agaaaaaagt attcctgcat gaaactgagc ggaccgtcgc cgggtgtcag
    16501 cggccggtcg tcggcgggcg gcgccaggtg acgctcgctg atcaactccc ccagttcggc
    16561 ggcaacccat tggacgccct cggcctgctg cgcggcggcc gaactgagcc gaccccagat
    16621 gccatgctcc tccagcaggc gcacggcgcg ctcggatcga aaaaccgggg atagcgtcat
    16681 cctgccaagc ctcgttgcgg gtgaatccgg ccgggatcga ccgcgaaggg caacagtata
    16741 gcggaaagca gccgttgatc gcaaacaggg aggcagccgc cggataaaag cccaaacccg
    16801 acccgcggct caatccagcc aaaggcgcgc gatcctcggt cgcctggacg gatcccggcg
    16861 accggcgact cgccgactcc gaggattaca cgccctgttc ctgttgcttc ttattgcgcc
    16921 gcgcgatccg gagcttttcc ttgacctcct gcgcctcggg gcgcaacgcc gccgcgatcg
    16981 acagatggtg caccgcctcc gaccagcgcg cctggcggaa ttcctccagg cctttgcggc
    17041 actcctgctg gaagagttcc tcggcctcgc tgcgcgccgc ggtcacgggg ggggccgccg
    17101 gttcgggggt cggggcgacc gtctcggcct ggctcggcgc cagctctacc agttcatcgg
    17161 ccggcagcag cgtctcgcgg tcccgttcca gattgaacgc cgtctcatcg gccgccgggg
    17221 gggcatcagg catccgctcc agatggatct cgacggtctc atcggaatgg aaaatgtcgc
    17281 ccggctgggg gcgttgctcg ccgtcgatga cctcgcccag cgactgggtg tcctccagtt
    17341 cggtctgcca cgaggtgtgg gtcacttcga acgccgagac ctcatcttcc tcgccctccc
    17401 cctcgtctag cagtccgccg gccagcgtct ggtcggcctc gggcccatcc tcggccgcga
    17461 gcgcctcggc ggcatcggcg tcgtattcga gcagatcgtc ggactgccag ggggcgtccg
    17521 gctccgcggc gggcggttcg gccttcggcg tcgacgaggg agtcggtgcg gcttcgcccg
    17581 catctacctc cagcaggtcg gtctgttcat aacgggccgt cggctcgcgt tgcgcgtccg
    17641 gcgcgacctc ttcatcctcc agcgaaatca cggcgtcggg cgggatgcgg tcctcttcgt
    17701 ccgatccggt cgcgggcaac tggtcgggat gcaggataag cggttcgatg ccctcggtcg
    17761 ggtgcggctc ctcctcggcc gccggctcaa tcgcggattc gcgcggcagc ggcagaacga
    17821 acgcatcctc cacctcgctg acggcatcga tcggctccgg cgccgcggat acctcgtcgc
    17881 gcggcaactc cgtccgcatc ggctccagct cgatctcaaa ttcggattct acggggggca
    17941 ggatgtcgga ggcctcggcc gcgagcgaat cgggcggggt ttcgccgagc ggataaatct
    18001 gatcctccag cgcgtcaacc gatccggagc gctgcgccgc caacgccgcc gtgatcggat
    18061 cggcgccggt ctgttcctcc ggcgtcagcg tgccgtcccc ctccagcacc agttcctctt
    18121 cctcgccgcc gcggcggacg accatccaga ccagcgcgat gaagatcagc gagacgacaa
    18181 cgacaactgt gatcaggatc ccctgccatg ggcggccctg ggcgggcacg gggggcagca
    18241 gcggagcggc cggcggggcg ggcgtcggca gcagggtcgc cggttccggg ccgggcgtcg
    18301 gcgtcagcat cgcaatcagt tggccgtcgc ccgcttcctc gacggtggga ggttcctcgc
    18361 cggcgaccgt ggcgtcggcc gcagcctcgg gcgactcggc cgcnnnnnnn nnnnnnnnnn
    18421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18481 nnnnnnnnnn nnnnnnnnnn nnncctcggg cgactcggcc gccgtcgccg tttcgatcgc
    18541 atcggccagc gcttcggccg cctcggccgg cgacggcgcc cccggcggct gcgtccgcag
    18601 gatcgcgcgg ggcgggaccg cgtcgggcgc cgacgccgcg ccggactccg ccgcgggttc
    18661 atcgggcgcg ccggcggccg cggattcctc atccgttgcc gggagctcgc ccccgggttc
    18721 ggccgattcc ggcgacgatt ccgggatctc gagcgcgggg cgttgggtgc tcagccggcg
    18781 gaacgggctg cgcccctcct cggcgtcgcg ccgttccttc aggcgcatcc gcgatgccgc
    18841 catgtagaaa tccgccgatt gcggcaagcc ggggccgttc tggagctgct cacccaggtc
    18901 cagcacccgc tggtactgct gcatgacgaa ggcgcggcgg atctcctcct ggaacgcctc
    18961 ggcctccgcc gcggagagcg gctgggggcc cggcgcgtcg atcaccaccg gcgtcgagct
    19021 ctggtcctca tactgcaccg ccggcagcgg cagcagcccc gtcgcggcgt ccatcggttc
    19081 ggttggctga gcgacggcca cacgcatgcc cgccagcgcc acggacagca acacaatccg
    19141 cgccgtcgcg ccggcaaccg ctgcggccgc cgctcggcgg gatgaaacca tgggacggcg
    19201 gatgcgcatg ggaaggcccc ctgaagggat gcggcaggtg agacgaaacc cgatcggacg
    19261 gggctgcaaa aacacggtgc agccaaaaac acgaacaact tacctcttat tgtgagcaat
    19321 cccgacgtgg acgtccacga aaaagcccgg ccgcggcccc aagcccgggt cttctggcgg
    19381 ggccggccag cggcccgcgc ggcgggtgcg cgccgcccgc cagacgcttg aaccggcctg
    19441 ttttatcctt ccgtttagcc ctttctcggt ctaacatgaa tcggaatctg ccgcaggtcc
    19501 tcccatccgg tggatgcgca tcgccccgat gcggaggcag ggcgtcggaa gggccgtgcg
    19561 ccgcgcggtc gcacggcgtc ggtcgccgcg gccgcgggat cgtccccgcc cgatcccctc
    19621 ggatcttgct tcattggctg gaaggagtcc cattcatggc gcgcctgttc gaaagcatcg
    19681 tcgatacgat cggccacact ccgttggtca agctgcgccg cgtggccgag gaggccggcg
    19741 cgcgggccga cgtctatctg aagctggaat tcttcaatcc gctcggttcg gtgaaggacc
    19801 gcatcggcgc tgcgatgatc gaggcggccg aacaagccgg caagatcgag ccgggcaaga
    19861 ccgtcctgat cgaaccgacg agcggcaaca ccggcatcgc gctggccttc gtcgccgccg
    19921 ccaagggcta cccgctgatc ctgacgatgc ccgagtcgat gagcatcgaa cggcgcaagc
    19981 tgctcaagat cctcggggcg cgcctcgtgc tgaccgaggg ctcgaagggc atgaagggcg
    20041 ccatcgaagt tgccgaggat ctgcaccgca aaatccccaa cagcttcatc ccccagcaat
    20101 tcgccaaccc cgccaacccc gacatccacc gccggacgac cgcccaggag atctgggagg
    20161 acaccgacgg tcgcgtcgac gtcgtcgtct caggcgtcgg cacggggggc accgtcaccg
    20221 gcgtcggcga ggtgctcaag cagaagaagc cgtcggtgca catcgtcgcg ctcgagccgg
    20281 tcacgtcgca ggtgctggcc ggcggcgagc ccggtccaca ccgtctgcag ggcctcggcg
    20341 cgggcttcgt ccccgaggtg ctcaaccgcg gcgtgatcga cgagatcatc gccgtaaccg
    20401 aggaagacgc cgcgcgcatg aaccgcgaga tcacgcgcaa ggaggggatc ccgatcggca
    20461 tcagttcggg cgccgccgcc tgggccgccg tcgagatcgc caaacgcccc gaaatggcgg
    20521 gcaagctgat cgtcgccgtc atgccctcgg cctccgaacg ctacctctcg gcgtggatct
    20581 acaacgatat cgaccttgaa agcgacagca ttgacgatct gctgtaatcg cgtcccgcgc
    20641 ccgtgatctt tttcctgcat ctgctctggc ggcacgtccg tcagtacccc acgcgggcgc
    20701 tggcggccgt ggtcgtcctg acggcggcct ccaccatcat gttgaccctg ctcggcctgg
    20761 gcgtggcgtt gcgcttccgg ctgggggact atctgaccga gctttttccg gaagagcagc
    20821 tccgcctgga ggcgcgccgc gccaatctgg gctacatcgc cctggagaat aacccgatta
    20881 cgccggagaa ggtggccgag ctcaaatcgc tgccgggcgt caatcaggtc tggtcggtcg
    20941 aaccgctccg cttcccgatc tacgccaccg gcagcatcct cggtcaggag ttggcatcgg
    21001 atgccgttgt gcatggcgtg ccgcgcgcgc tggtggccga ttcgcttccg cccgacgccc
    21061 cctggcggat gcccgacgat cccgacgccc cctgcccggt catcgtgtcc agttactttc
    21121 tggatcttta caatctgggg cttgcgcgct ccagcggtct gccggcgctg agcccctcgg
    21181 ccctggtcgg gcgcgatttc acgttcgtga tgggcgaatc ggtcctcggg ctgggcctgg
    21241 catcggacgt gacgccggtt caggtcaggg cgaaggtcgt cggcctctcc aaacagcccg
    21301 ggatgctcgg cctggcgatt cccgccgaca tcgcgcgggc ctacaaccac aagttcgccc
    21361 ccggcacccc ggaaacctat gcccaggtcg tcgtacaaac ggctcgcgac gccgaccgcc
    21421 aggcgatcct cgcccgtgcc ggcgagatgg ggctgcaact ggccggcggc gagctgctcg
    21481 gcgaacaact caagttcggc gtgcgcctga ccggctgggc cgtcatcgcg ctggcgctgg
    21541 cgatcttcgc cctcggcatg ctgacgttct acctcctgtt cacgatgatt ttccacgcgc
    21601 ggcgcccgga cctgatcgcc ctgcgcacgc tgggcctgac ccccgcgcag gccgtcggcc
    21661 tggccctggg cgaggtgatt ctgctcgcgg cgaccgccgt cgcgctggcg ctggccgcca
    21721 cggccggcct cgccgcctgg gccggggcca cgatggcgcg ctggaccgag cacctgagct
    21781 ggctgccgga ggacttgctg cgcccccagc ccggctggct gctggccggg gcggcgctga
    21841 tcctgggcgt cacgctcctg ctggcgacgc cgatgctgcg ctgggtggtg acggtccgcc
    21901 ccgcggccgt catccgcgat cgctagcggg aactcccacc cagccgactg gccgcaacgc
    21961 cgcggacgcg gaggaaaccc ggatgtgcgg acgctttgcc gtctactcga cgattgagga
    22021 actggccgag ctgattcggg aggcgctgcc cgatctggaa atcgagcagg cgagggggtt
    22081 cgttccgtcg tacaacatcg cgccctcggc cgcgatcgtg acgatcggcc ggagccggcc
    22141 ggagcatccg caccggatgg cgctgctcaa atgggggctg gtgccgagct tcgcgaagga
    22201 cccgacccgc atggcgccga tcaacgtgcg gtcggagacg gtgctgacga aaccgatgtt
    22261 ccgccggccg atcgagcgcc agcgctgcct ggtgccggcc aacgggttct ttgaatggaa
    22321 acgcgcgggg agccgcaagc aaccctattt catccggcgc gccgacggcc gaccgctgct
    22381 gatggcggga atctacgacg tgtggagggg cggcgagggc ggcgcgccgt tgatgacgtg
    22441 tgcaattctg acaaccgaca gcaatgattg gatgaaaccc attcatcacc gcatgccggt
    22501 gatggttgcg ccggaggccg cggcgcgctg gtgcgacccg cagcgccccg ccgccgacgt
    22561 cctccccctg ctgacaccct cgcccgccga cgcgctcgaa gcctatccgg tcgacgcccg
    22621 gatgaacagc ccgcgccacg acgacccggc gtgcatcgcg ccgctgccgc ccgcgcccga
    22681 cgtccccggc gacaaggtgg acccggagca gaggtgatcg aggctgtgca gtccggcctc
    22741 agtcagccgc cgccgttcca acttcgcccg gcgccggcgg gagggggagc tggacgaccg
    22801 gcgcggccat cacgtcgcgg atgcgttccc aaagccggtc ggcggcgaac gggtcgatcc
    22861 gccgataggc ctcaacggcc cgccagacgc gcgcccgggt ccccgcgccc ggtcgcagca
    22921 gaacgttcat cgcctcggcg gcgcgcgcgg tccggccggt ctgcaaatag cattcgatca
    22981 agtcgggaac gtaatcggcg ccggcgccga gcgggtcgcc gtcgaacaca gacggttcga
    23041 gcagctcaat tgccttgtcc cgccaactcg acatcgaccg atagtcggaa ggggaccgaa
    23101 agccgcgccg acgaccgctt cgcggcctct gccgcgccgc aggcaaatcc ccccaagcag
    23161 cgaggccgcg tcggcggttt agagccagct tcatctaccg cgtaagaagg gtcgcattcg
    23221 gcgggcggtc ggcgttagga tggagccgcc ggcgttccgg ttccctgatc tcccgctgcc
    23281 ccggcgcctg tcagccatga ccgcactgct tcgacgtttc acgcaaacgc cgtcggccgc
    23341 tccacccgcg gacccgcggc tccacgtcgc cctggacgtc cggttctggt cgatgagcgg
    23401 catcggcacg tatgtgcgcg agctgctggc ggcctacgcg cggctgcggc tgccgctggt
    23461 gctgacgctg atcgggccgg aggggttgcg cgaccggatc gcgccggggc tggagatccg
    23521 ccgctggatc cacttcgagc cgccggttta cagcgcggcg agcttcttcc gctatcccgc
    23581 gctgccaggg gtcgacctgc tgcactaccc gcattacaac ctgccgctga cccgcgcgcg
    23641 ccacaagctg gtcacgttct acgacctgtt ccacctgcgc tacgggtcgc tgcccaagcg
    23701 gcgctaccag gactttttcc tgacgcggct gcgctggagc cgggcccaca tcgtcgtgcc
    23761 gagcgagaag accggtcacg atctggaggt ggccgggttg cgcggccggc gggtgagctg
    23821 catcccgctg gggccgggcc agagtccgcc ggagcggccg gcgacgccgc cggaggacct
    23881 gctgacgctg accggcgcgc cgccgcgccg ccgctggctg ctgacgaccg gcatcgatca
    23941 gccgcacaag aacatcgatt tcctgatctc ggcgctgagc ctgtattatc agcgccggcc
    24001 cgacgcgccc ccgctggtgt ggtgcgggtt gagcgcggag gatcgcgacc ggcgggcgcg
    24061 ccagatcccg gcgcacatgc gccagcacgt cgtgctcgag ccgttcatcg acgccgagcg
    24121 gctggagagc ctgatggcgg gggcgctggc gctggtcttt ccgtcgctcg acgagcgctt
    24181 cggcttcccg ccgctggagg cgatgacccg cggcgtgccg gtcctctgcg cgcggcgcga
    24241 gccgatgagc acaatcctgg gcaatgcccc gctctacttc gagccgaatg aatcggccag
    24301 cctgtggcgc gcgatcgacc ggctgctggc ccccggcacg gtgcgccagg aactgatcga
    24361 tcgcgggcgg caccgcgcgg cgcagtacag ttgggacctg acggcgtggg acaccgcgcg
    24421 gctgtattac gaggtggccg gccgcgaagt gaccgtggaa ggcgaagaga cccctgaacc
    24481 cgacgacgcg gccgcggtgg aacagccgcg gcaggcgtaa tccccgccct tcctcctgca
    24541 atcgcaatcg ctatcgggat cggtatcgcg atcgtaccgg cgttcccccg ccacccccgc
    24601 gcctcactcc aacccgcact ggcgataaat ctcatcgacg tatttcagga agtcgttggg
    24661 attcatcagg cgttccagct cttcgcgcgt gaagcgggcg gcgacctcgg cgtctgccag
    24721 cagatgttcg atcagcggga cgcggtcctc ccaggtccgc atcgcattgc gctggacgat
    24781 caggtaggcg tcctcgcgcg aaatgccctt gtcgatcagc gccagcagga tgcgctggct
    24841 gtagatcagg ccgccggtct tgttcaggtt ttcggccatg cggtcgggct tggcctgcgc
    24901 cccctccagg atgcccgcca gccggtgcag catgtagtgc agcagcgtga gcgagtcggg
    24961 caggatgatg cgttcggcgg aggaatggct gatgtcgcgt tcgtgccaga gcgcgacatt
    25021 ttcgagcgcg acgaccaggt tgccgcgcag cacgcgggcc aggcccgtca ggttttcgca
    25081 gccgatcggg ttgcgcttgt gcggcatcgc cgagctgccc ttctggccct tgcggaacgg
    25141 ctcctccagc tcatagatgt cggtgcgctg caggttgcgg atttcggtgg cgatcttttc
    25201 gatcgtggcg ccgcagacgg ccaccgccgc cagcgcctcg gcgtgccggt cgcgctggag
    25261 cacctgcgtg gcgatcggcg ccggccgcag ccccagcttc tcgcagacgt agcgttcgat
    25321 cgcgggcccg gtgtgggcgt aggtgccgac ggcgccgctg atcttgccga cggcgaccgt
    25381 ctccttcgcg gccagcagcc ggcgccggtt gcgcagcatc tcctggtgcc agagcgcgaa
    25441 cttgagcccc aggctggtcg gctcggcgtg gatcccgtgc gtgcgcccca tgatcggctg
    25501 gtgtttgaac tcgacggcgc ggcgggccag cacggcgatc acccgctcga gctgctccag
    25561 gatcaggtcg aagctgcgcg ccatgcggac gctctgcgcc gtatcgacga cgtcggtcga
    25621 ggtcagcccc atgtggacga aacgggccgc ttcgccgatc tgttcggcca gggcggtcgt
    25681 gaacgcaatg acatcgtgat ggacttcggc ctcgattgca tcgatccgtt cgagcgtgaa
    25741 ggcggctttc gccttgatct gcgcgagcgc ctcggcgggg atcgctccct gctgggccca
    25801 cgcctcgcag gcgagcagct cgacctgcag ccagatctca aacttggtct ggtcgctcca
    25861 cagcgcccgc atttcgggca gggcgtaacg ctcaatcatc ctgtcgggtc tccagtctca
    25921 atgcgggttc aaccgggcat gcaggaacgc gttccaacgg ggggcgtcga gcacgcgcat
    25981 ctcgatttcg acgacgatga ccggcaggcc gaacatcggc agatgctgca gcttgacgcc
    26041 gtccttggcg gtggtcagga tcagcggcgc ctcgcgcccg gcctgttgtt cctcgtcgcg
    26101 caggcggcgc cacgcatcca cgtcggcggg gccatactcg tgatgatcga aataggcgaa
    26161 gtggcgcacc acgcgaccgc cggtctggcg cgccagcgac tccagcccct gcggccgggc
    26221 cagggcgctg accaggatca ccggccggcc ggtccacggc caggggccgt cggcttcggc
    26281 cggcggctcg gcgtgcgggc cgacgcgccc gctcaggcga cggaagccgg cgacgactgt
    26341 ttcggcctcg aaaatcggaa cggcgggggc atggcgcttg agcgcggcca ccgtttgttg
    26401 gcggcgcccc tcgcgctcct ccggcgccag gtactcggcg tgggtcagaa tgattgcatc
    26461 ggcgcggcgc aacgcgccgg gccgctcgcg cagccgcccg gccggcaaga gcggcgtgcg
    26521 gtcggggtcg tcgcgcagat cccacagcac cagatcgagg tcgcgcgcca ggcggtcgtg
    26581 ttggaagccg tcgtcgagca ggatcacgtc gggatcgaac tgctggatgg cgagccggcc
    26641 ggccgccgcc cgatcgggat ggatgatcac cggcacgccg ctgcattgct cggcgagcag
    26701 ccgcggttca tcgcccgcct cgctgggcgt ggaatagacg cgcgtgccgt cgctgacgac
    26761 gaccgggccg ttgaggtgaa cccgcgcgcc atagccgcgg ctgacgatcg ccgggcggcg
    26821 ccccatcgcg ctgagcgtcg ccgccatcaa ccggacgaac ggggtcttgc cggtgccgcc
    26881 gacggtcagg ttgccgacgc agatcaccgg cacggggagc gccttgcggc cgccggggcg
    26941 ccagatgcgc gccgcgcgat ggacgctgcg gccgagccca tagagatatc cagccggccg
    27001 ggccagccag cgcgcggccg gctccagcgt gtcgagcccc gcccgcaggc ggggggcgtc
    27061 gatcagccgc cggcgctgca gcacccgggc gtcgacgcgg atgagctcat cctgcgcctt
    27121 gcgccgtcgc ggccagggaa acgccagagg acggatgggg acggtcgcgg cgggggcggg
    27181 gggcgatgag tccctcggat cggcgccggc gccggccgcg cgcgacccgg cagccggccg
    27241 aacggccgcc gccagccggc cggcgaagcg ccgcacgccc cgcccggcgg cacggagccg
    27301 gcgtcggcgt cgttgccgcc gcctgcgcgc cagatcggag ccctcgggca ccagcgcgcc
    27361 gggacgcagc cagagccgga cgatcgagcc cagcgagcgc agcatgatgc gcagattgaa
    27421 attggagcgg ccggcgcgcc ggtcgacgaa ccggatcggg atttcgacca tgcgaaaccc
    27481 gcgggcatcg gccgccagca tcagctcctg caggatctcc ggcccctcgg cgcggatcgc
    27541 ctcccacggc atcgcctcca ggcaggcgcg ggtgtagtag cggtagccgg tcgtcgtgtc
    27601 gcggacgggg aactccagca gcaggcggat ataggcattg gcggcccagg tgatgatcgc
    27661 gcgcagcggg tggcggccgg cctcgccgcc gccgtcgacc cggcgcgagc caatgacgat
    27721 ggcggcgccg gtgcgctcgg ccgccgcgat catgcgcggg atgtagcgcg ggtcgtgcga
    27781 ccagtccgca tccatttcga tcacggcatc ggcgcccagt tcgagggcct tgcgcatgcc
    27841 ggcgacgccg gccttgccgc ggccgcggtc ggtcttgcgg tagagcaggt gcaggcgggg
    27901 ctcctcggcg gcgcggcgct ggacgagcag ccaggtcttg tcgggcgaat cgtcgtcgac
    27961 gaccaggatt tcgacccgcg gttcgagggc gagcagcgcg tccagcagcg gctcgatgtt
    28021 ttccgcctcg ttgtaggtgg ggagcatgac gatgacgcgg gacatgggca atcgggaacg
    28081 gagaaatcga gccgggcgga ggccgcaacg cgccgaccac tctaaaagac ccgcgccgat
    28141 tcttcaatcg ccgcggggag tcgcctggct tgccgaccgc ggagaagtga gtatcggaat
    28201 cggtatcgac tcccacggtc ggttccaggg gccaacgtca tgggcttcgg ctccgccgcg
    28261 ccgcgcggga tgcggttgaa taacgcgacg gctccgtata gaatcccggc tgcgaatcac
    28321 tgagctgccg cgggggccgc cgcaacgcaa cgctcgccca cccggcgcgc cgcgtttccc
    28381 gcgggccggc gcatccgatc cacgcgccgg acgggcacgc cgcccgacga gcggcaaacg
    28441 gccatcagga gcggacgatg attgtcgggg ttccccggga gactctggcc gacgaaacgc
    28501 gcgtggccct gacgcccgcc gtgctgccga tgctggccaa ggcgggcctg agcgtggcgc
    28561 tcgaggccgg cgccggcgac cgggcgggtt ttccggacgc ggcgtgggaa gccgccggcg
    28621 cgcgcgtggt cgccggccgc gacgaactat tccgcgaggc cgacatcatt gcgcaggtgc
    28681 tggcgcttgg cgccaacccg gaagcgggcc gcgccgacct ggcgcggatg cggccggggc
    28741 aactgattgt cggcatgcat gatccgctgg gccggcccga ggcggcgcgc gagctggcgg
    28801 cgcgcggcgt gacggcgctg gcgctggagc tgatgccgcg catcacgcgg gcgcagccga
    28861 tggacgtcct cagctcgatg gcgaccgtcg ccggctacaa ggcggtgctg ctggcggcgg
    28921 cggcgctgcc gcgcatgttc ccgatgctga cgacggcggc cggcacgatc aagccggcgc
    28981 gcgtgttcgt gatcggcgcc ggcgtggccg gcctgcaggc gatcgccacg gcgcggcggc
    29041 tgggggcggt cgtgcgcggt tacgacgtgc gccccgccgc ccgcgaacag gtcgaaagcc
    29101 tcggggcggt cttcgtggaa ctcccgctga cgacggccga tgccgagggc cagggtggct
    29161 acgccagggc gatggacgag gatttctacc gccgccagcg cgagctgatg gcgaaggaag
    29221 tgaccgccag cgacgtggtg gtgacgacgg ccgcgatccc cggcaaaccg gcgccgcggc
    29281 tgatcacgcg cgagatggtc gaacagatgg cgcccggctc ggtgatcgtc gatctggcgg
    29341 cgcccggcgg cggcaactgc gagctgaccc ggccgggcga agaaatcgcc caccagggcg
    29401 tgcggatcat cggcccgctg aacctgccgg cgacggtccc ccacgacgcc agcatgatgt
    29461 acggccgcaa tgtcgccacc tgcctggcgc acctggtccg gcagggcgaa ctgaagctcg
    29521 acgatgacga cccgatcgtg cgcgagacga ttatcgcgcg cgacggccgc gtcgtgcatc
    29581 cgcgcgtact ggaggcgctg ggcgaggcgc cccccgcgca accggccagg cagacccccg
    29641 ccgcgccgaa ggagggctga tccgatggaa ggcttcatcg ccgcgctgac gatcttcgtg
    29701 ctggcgattt tcgtcggctt tgaagtcatc accaaggtcc cgccgacgct gcacacgccg
    29761 ctgatgtcgg ggtccaacgc gatctcgggc atcacgctga tcggcgcgct catcgccgcc
    29821 ggcatgggtc acacctggct gacgacgctg ctcggcgtgc tggcgatcat tctggcgacg
    29881 atcaatgtcg tcggcggatt cctcgtcacc caccgcatgc tggcgatgtt ccggaggaaa
    29941 cgctgacctt gagcgcaacc tggcaaaatc tcgcctatct gatcgcggcg gtcctgttca
    30001 tcctggggtt gaaggggctg gcgcacccgc gcagcgcccc gcgcggcaac ctgctgggcg
    30061 ccctggggat gctgctggcg atcgtcgcca cgctggccgg cggcggcatc atcggttggg
    30121 gctggatctt cgtcggcctg gcgatcggcg gggtgatcgg cctcgtcctg gccctcaagg
    30181 tgcaaatgac ggcgatgccc cagatggtgg cgctgctcaa cggtttcggc ggcgcggcgt
    30241 cgttcctggt ggccgccacg gcgctggcgc tgcaggccga agtccccgcc ggcttcacgc
    30301 tgcaattcac gatcgccacg gccgcctcgg gcgtcgtcgg ttcggtgacg ttctggggct
    30361 cgctcgtcgc gttctggaag ctgcagggcc tgccgggcga aaactggcag ggttttcccg
    30421 gcgttcaact ggtgaatatc gtgctggtgc tgctctgcgt ggcgctgggc tggatcatcg
    30481 tcgcccagcc gccggcgatc ggggtctact ggctgctcgt gctggtggcc gccgtgctgg
    30541 gcgtgctgct ggtcgtgccg atcggcggcg ccgacatgcc ggtcgtgatt gccctgctca
    30601 actcgtactc gggcgtggcg gcggccgcga ccggcttcgt gctctctaac agcgtgctga
    30661 ttatctccgg ttcgctggtg ggggcgtcgg gcctgatcct gacggcgatc atgtgcaagg
    30721 cgatgaaccg ttcgctgccc aacgtgctgt tcgcgaagtt cggcggcgaa tcgagcgccc
    30781 ccaaagccga cgacatctac gagggcaagg tcaaggcgac gacggccgag gaactggcga
    30841 tgatgctgga ggatgcccgc cgcgtggtgt tcatccccgg ctacggcatg gcggtcgcgc
    30901 aggcgcagta tgccgtgcgt gacctggcca atctgctcga gagccgcggc atcaacgtcg
    30961 agtatgcgat ccatcccgtc gccgggcgca tgccgggcca catgaacgtg ctgctggccg
    31021 aggccaacgt cccctacgaa aaactgcgcg aaatggacga catcaacccc gagatggcgc
    31081 aggtggacgt cgcgatcgtg ctgggggcga acgacgtggt caatccgctg gcgcgcgccg
    31141 acggcaacag ccccatcgcc ggcatgccaa tcatcgacgc cgacaaggcg cgccaggtcg
    31201 tcgtggtcaa gcgcagcctg agccccggct atgccggcat ccccaacccg ctcttcgcgg
    31261 ccgacaacac cgtgatgttc ttcaacgacg gcaacaaggc ggttcaggaa atcatcgcgg
    31321 cggtaaaagc gacctaggtg ctaaggctga ccccacgaaa aaggccaccg cgaaggtggc
    31381 cgatgttgcg taagtccttt attttctggt acgcccgaca ggattcgaac ctgtgacctc
    31441 gtgctccgga ggcacgcgct ctatccagct gagctacggg cgcacagcag acgaatccct
    31501 atcgtagcag ccccccggtc cgagtcaagc aattcggtcg aacccgcgaa gcgaggcgcg
    31561 ccgcctcaat ggcgtccccg ccccgggagc gcgtgacgcg cgccctgtct attcctgatc
    31621 cgcgtcgatc caggcttcat cgccgtcggc cgagagggtg acggtcagcc ggttggggcg
    31681 gcagcagacc tggcaatcct cggtgtaggc ctggcgcgcg ccggcgctgg cgtcgacggt
    31741 cgtcgggttg ggctcgccgc accacgcgca aaggaattcg gcgtcgatct gcatgacgcc
    31801 attgtgccgc aaacccgccc gccgcgccat gcgcgatctc gagggcgtgg ggggtagggc
    31861 gggattcgat tccgatcccg atcccgatcc cgatcccgat accgataccg atcctgatct
    31921 ccggacccgc aagccttgca tctggcccgg ccgcgcgata cggtatccat ttgcggcaag
    31981 gggggaaacc ctgggcgcgc ccctcgccgc agccacggcc gggcggcttg cccggctccc
    32041 cgtcaacttt ggaggccgtt caatccatgg cgatgcgtat tctgatcagc gacaaaatgt
    32101 cgagcgaggg cctggccaac ttcgaaggcc acgctgattt tgagacggta tacaagcccg
    32161 acatcacgat ggacgcgctc gccgaggaga tcggccagta tgacgcgctg gtgatccgca
    32221 gccggacccg cgtgacgcgc gaaacgctcg cccggccggg caagctcaag atcatcggcc
    32281 gcgccggcgc gggggtcgac aacgtcgaca tcgacgccgc caccgagcgc ggcatcatcg
    32341 tgatgaacac gccgggggcc aacacgatct cgacggccga acacacgatc gcgatgatga
    32401 tgacgctcgc ccggcggacc gcctacgccg accgcacgat gaaggagggc cagtgggcca
    32461 agaaggacat cgtcggcgtc gagctgagcg gcaagacgct cggcgtcatc ggcctgggca
    32521 agatcggccg cgtcgtcgcc gaacgcatgc gcgccttcga gatgcgcgtc ctgacgtttg
    32581 acccgttcct cacgcacgac caggcgcaca agctggggat cgaaccggcc gacgtcgaca
    32641 cgatctgccg cgaggccgac ttcatcacga tccactgccc gcttaacaat gagacgcgca
    32701 acctgatcaa cgccgagcgg ctggcgttga tgaagccgac cgcgctgttg atcaactgcg
    32761 cgcgcggcgg catcgtcaac gaggacgacc tgtacgaggc gctggcggcg gggcggatcg
    32821 ccggagccgc gctcgacgtc ttcacgcagg aaccgctccc cgccgatcac aagctgcgca
    32881 cgctccccaa catcgtgctg acgccgcacc tggccgcctc gaccaacgag gcgcaggaga
    32941 aggtctcgcg cgatatcgcg gtgcagatcc gcgaggcgct cgccggcggc gtcgtgcgca
    33001 acgccgtcaa cgcccccacg gtcgacccga agacctacga ggcgatcggc ccggcgatgg
    33061 acctctgcct gcgcatcggc cggttctgct cgcaatacgc ccaggcgccc gtcaaggaac
    33121 tcgacatcac ctacagcggc gccaaggcca agcacccgcc gacgccgctg acgaccgagc
    33181 tcgtccgcgg attcctgcag tacagcttca gcgagccggt caacgccgtc aacgcgctgc
    33241 acatcgcccg ccagttgggg atccggatca tcgagacccg catcgacgag tccgacgacg
    33301 tctacgccgg gctggtgacg gtgcgcacga ccgacgagaa gggcgaggtg aacacgctca
    33361 gcggcgcgct gaaccacgcc cgccagccgc gcttcgtgat tttcaatgaa aagcggatcg
    33421 acgccatccc cgaaggccac atgctgatca tcgaaaacgt cgacgtcccc ggcattatcg
    33481 gttcggtggg cacctgcctg ggcgagcaca agatcaacat cgcctcgatg aactggggcc
    33541 gcgtcgcgcc gggcggcgac gcgctgaccg tgatcaacac cgaccagccg attccgaacc
    33601 acgtcctgga atgcctgcgc gcgctgccca acgtcctcgg cgcgcggcat gtggtgattt
    33661 agcttgaccg ggctggaggg ccgacgccaa gtcgtgggca tcgaactgct gaatctttcc
    33721 cgccgcacgc ccagactgga tctgcaaaag cttgcgtttt tgaccgcctg acgctccgct
    33781 ccggccgctc ctacaaggcc tcgagccacg accgcatcgg cggcgcgagg gattccgcca
    33841 gccgcgcatg cccttcgatg tcgaggcatg gatcttccga tcggtcccgc gaccgcgtcg
    33901 tgttgggtga gggcggatct cccgccgaat cgtccggcat ttcggccgca tcccacaccg
    33961 gcaggccgag atccgccaac cgattcaggg cggggtcccg ctctccgccg ccgtgttgcc
    34021 catccggtgc gacaaagatc gcaagtccca cgtccgccgc ggcgcattcc gctttcagct
    34081 cggcataggc ttgcgccatg cggtccagat cgcccaaatc cgccctttgc cgatccgaag
    34141 acggctgccc gcccactccg gtggaaaacg gcagcggatc aaggccgcga cgaagccccg
    34201 ccggtgtgcc gagatacgtc gtgaagacgt ccgacaggac cgcgccaaac aaatcgcgcc
    34261 gtgtcagaaa atccgaatgc gccaacagcg gaatcggcgc ttcatcgaaa agccggtgct
    34321 ggatgatgac gccctccacg tccaactcgc gcgccagccg gccggccagc gcggctttct
    34381 ggatccccgt gtatccgggt atgcccgcgt tgatgagttg aaccggccgc gcgtccgcgc
    34441 ccacgctctg gttcagcatc ctttcggtcc gtgccacata gccgtcggcg gcctcgacgc
    34501 cggcgccgaa cgtcacttca tcccccacca ccaggatgcg ccggaattcc ttccgcttca
    34561 gggactccag ctcctcatcc cgcaagccga gttcattcaa ttcaaagggc gcgcccgcga
    34621 actcgccccg gagccgcggc gtcagcgtat agaccacatc cggatcggga tcgggcgcga
    34681 gcatgctccc cagccagtga tgttcaatgg caaagggtga attcggaatc atgccttcgg
    34741 caaactcgct cgcgacggcc cgccgcagat ccatcacggc atcgattcgg gccgtcgccc
    34801 ggtcgcgctc ctcctcgctc agtcccgtcc aatcgatcag cgggaggatg ctgtcggcgt
    34861 tccggaccag gttgctgatt tccgcccagc cccccgtccg cagcggccgg gtcacggcgt
    34921 cggaccgctg atagaagata tcgaatccga tggcggagtg cagatgaatc agaccatatc
    34981 cggacagggc gctgcgggga ttcgtgaaat cgatgcgggt ccgatcgtcg gtgatcgtct
    35041 gcgcgccgcg gcaatagcgc gcaatcgtct cgggccgcat caacagcagc gccagcagac
    35101 tggccgggtc gcgcagattg ttgcgctcca gattttccgc cagccgcggg tcgccctgaa
    35161 tgcgttcata catcccggcc agatcgacat gcagcggttc ctcggtcccc agaaagatga
    35221 agtgcccgaa caccggggtc tgccagacgg cgccgttggg aaagacccgg tgaaacgccg
    35281 tgatcgtctg gcgcaattcc tcgcgcccca gcatgaaggc gttgatccac atcgaaagga
    35341 tgccgcccgg cttcagccgg ctccgggcga tccgatagaa ttcctcggtg taaagaaaga
    35401 ccaccccggc gctgtgcagc tcgggcggct cgatctgaat cacgtcgtag tcgtagcgcg
    35461 agccgagcag gaagctgcgg ccgtcgtcga cgtggaggtg gatgcgcggg tcccgcggcg
    35521 gcgcgaggat cccttcgttg ctctcgaacc aggtcgatgc ctcggcgatg ccggcgctca
    35581 gatcgaccgc atccaggcgt tccaggctct tgtgcgcggc catcgcggcg atcgtgttgg
    35641 cggcgccgat acagatgacg gcggcgacct tcgggtcggc atgcagcaac atcggcaggt
    35701 ggccgttgaa cgcattgtcc tgccgcgtca aatgccccgc cgagccccgg ccgtcgttga
    35761 acaccagcca gcgctgctcc ggccggggcg tccccgtcga atcatacata tcgacgacca
    35821 tgagggtgtc ggtcacttct tccttgtaga acaccacctt gcccaggcgt tcggcgaaaa
    35881 cccgcatcag cacatcccgc ggaatgccga cggccgcaac gatcacgagc aggacgcaac
    35941 ccgcggccgc gcagacccgc cagcccgacc gtggcggatg cccggcgagc aggagcacgg
    36001 ccgaaacggc cgcgaggagg ccgcccagca gaatcgccga ctcgcgcacg ccgatggtgg
    36061 atagcaggat gaagccgatc gcgaacgccc ccgcgaccgc gccgaccgta ttggcggcgt
    36121 aggcgttgcc gaccttccgc ccggcgctct ccgcgccgac catgatcagc cgcgtcgcga
    36181 gcgggagcgt catccccatc agcgtggtcg ggatcagcag cagcgcggcg ctctgaatga
    36241 aaatcaacgc cagcgcctcg taccagttgt cccatggcag ttcgcccgcc agcagccggg
    36301 aaaaccacgt cagcttcccg ttgagcagca gcgtcaggat gcaccacagg ccgatcagcc
    36361 cctccagcac cgcgaacagc gtcaggggac gccgcaggcg cggcgcgatc catcccagca
    36421 gccagctccc gccggacagg ccgatcagaa acaccatcag aatcgcggag aaggcatagg
    36481 tggaattatg cacatgaaac accagcatcc ggttccagag gacctgcatg cccatcgagc
    36541 agaatccgga caggccgatc gccccgagca cgagctgacg ccggccggcg ctcagatccc
    36601 acgccgcgac gtgcgtcccg gtttcgtgga gactgggttg gcgcggctcg gactgcggcg
    36661 tcggccgcga ggggcgccgg tcgagcagga tcgcgccggc gccgatcagc aggttgatcg
    36721 ccgccgccat gaggatcgtc ccctgcatgc ccagcacctc gaccaggatg aagccggcca
    36781 ggaacgtgcc gaggaccgcg ccgaacgtgt tcgcggcata gaggaagctc agttcgcgcg
    36841 ccaccgccag cccgccccgc gcgaagaagc gatagaccac cggcagcgtg ccccccatga
    36901 aaaacgtcgg cagcagcatc agcggcagaa tgatggcgaa gcgcgcgatc gagaacagca
    36961 gaaaatcttc gccgatctgg cgattgatcc acacgaacag gggttcgatc tgatcgatca
    37021 accagggaaa agccagcgcc gtcgcgccga tcgtgatctc cagtgcgccg tagagtcgca
    37081 agggtctgga tacccggtcg gcgagccgac cgaggaccca gcttcccagc gccagtccga
    37141 gcatgaaggc gcacagtacg gacgtaatgg catagacact gacgccaaag accagcgtca
    37201 gcatccgcgt ccagacgatc tcgtagatca ggccgcacgc gccggaaccc agtatgagga
    37261 cataaacgaa cgatgaacga cgcgcagtga ggagggtgtt ttctgattca ggggagagaa
    37321 gagcaggaat ggcccgctcg ggatacggct tgagagggag accatctgga gattcgtcgg
    37381 atggggccgc ggcgcccgct ggttgttcgg aatcgggtcc ggattcgtcc aatgccgtga
    37441 gggtggcagc gtgatccgga cgcggcgccg gtgcgtcggc cattcccccc tcatccaaag
    37501 ccgaaccctt ccggcgcccc ccgttcacat tgagcacccc cccccgattt tttcgacaga
    37561 caaaagaccg tcgtcccact tcctgtcttg agataacaat taaccgatta ctgattaacc
    37621 gtcaaccgcc gagtgagcgc ccgtccgagt ggatggcgat ggcgcacgcc cctcgccgcc
    37681 gggaaggcgg ccgattcgaa acggcgtcga tcgcgatagc gataccgatc ccgatcccga
    37741 taccgatccc gatgcagatg cagattgcga tgccgaggaa aaagtatcga ctgtacggcg
    37801 attgtgctaa tagtgtcagg ttgagggccg ccgaacggct cgaccgatat cgcccgggat
    37861 ggtagacgac gcacgatggc gcagtggctc gaacgcattc aggaaccgcg cgacatacgg
    37921 ggactcgcgc tggacgactt gcggcagatc gccgaggaaa tccgcgaaac catgctgcgc
    37981 gtcacggcgg tcaacggcgg tcatctggct tcctcgatgg gcgccgtcga gctgatcctc
    38041 gcgctgcact acgtctacga cacgccgcgc gaccggctcg tcttcgacgt cggccaccag
    38101 gcctacgccc acaagctctg accggccgct acgaccggtt ccacacgatc cgccgcgagg
    38161 ggggcctctc cgggttcctc aagcgcagcg aatcgcccta cgacacgttc ggcgcgggcc
    38221 acgcctccac ttcgatctcc gccgccgccg gcatggcgat cgcccgcgac cagcgcggcg
    38281 aggactacaa ggtcgtctgc atcaccggcg acggggcgat gtcgggcggc atctgctacg
    38341 aggcgctcaa ccacgccggc atgctcgaga ccgatctgct ggtgatcctc aacgacaacg
    38401 aaatgtcgat ttcgcgcaac gtcggcggcc tcgcgcgcac gttcaaccgg atcgtgacga
    38461 cccacttcta caacgagaag cgccacgaca tcatggagct gatccgccgc ctgcccgccg
    38521 gcgagcggtt tctgcgcatg accaaccgca tcgaggaatc ggtcaagggc ctgatcctcc
    38581 ccggcctgtt cttcgaggag ctcggcttcc gctacctcgg cccggtggat ggccacgacc
    38641 tggaggggat gatcgaaatc ctgcgcaaga tccggacgtt ccgcgggccg atcctgctcc
    38701 acacgatcac gcgcaaaggc aaggggcggc cctacgccga ggccaatccg attctgtggc
    38761 acagcccgcc gcgcaatttc gacgccgaca gcggcgaagc ccccgtcgtc cagcccggtc
    38821 cgcccagcta cacgaatctg ttcgtcgatt cgctcaagcg ggaggcgcgc cgcgacccgc
    38881 gcatcgtcgc gatcaccgcc gcgatgctcg aggggaccgg cctggtccag ttcgaggagg
    38941 aattccccga gcgcacgttc gacgtcggca tcgccgagga gcacggcgtg atctgcgccg
    39001 ccggcatggc ttgcgacggc ctgcgcccgt tcgtctgcat ctactccacg ttcctgcagc
    39061 gcgccttcga tccgatcatc cacgacgtct gcattcagaa tctgccggtc gtcttcgcgc
    39121 tcgaccgcgg cggcctggtc ggcgacgacg gccccacgca tcacggcgtg ttcgacctct
    39181 cctacctgcg catggtcccc aacatggtcg taatggcgcc gatggatggc gaggagctgc
    39241 gccacatgac gcacacggcg gcgctgtacg aagccgggcc gatcgcgctg cgcttcccgc
    39301 gcggctcggc cgagggcgac atcagcctcg atgccccggt ccagcagctg ccgctgggca
    39361 agggcgtctg gctgcatgac gaacccgacg gcgcgccggt ctgcctggtc ggcatcgggc
    39421 tgatggcggc gcacgcgcgc aaggcggccg acctgctcgc cgcccgcggc atccgcgccg
    39481 gcgtgatcaa tgcccggttc gtcaagccgc tcgaccgcga gctgctgacc gacgcggcgc
    39541 ggcgctacga ctgcctggtg acgatcgagg acaacgtgct cgccggcggg ttcggctcgg
    39601 ccgtgatgga gttgctcgcc gccgacggcg tcgacgtccg cgtgctgacg ctcggcatcc
    39661 ccgaccggtt catcgagcac gccaaacccg acagcctgca tcgccagctc ggactcgatc
    39721 cggcggggat cgccgcgcgc gtcgaggcgt tcctggcggc gcggccgcgc gtccaggagc
    39781 tcgccatcga acggcgcgcc gaattcggcg gccgctgagc ccgcgccccg tcaggcgcaa
    39841 accaggtttc gccccgactc cttcgcccgg taaagctgcg tatcggcgcg cgccatcagg
    39901 gcggccgcat cctcgcccgc gcgccattgc gccacgccca ggctcgccgt catccggaat
    39961 ccctcgccct tgcgcgtttt catcggcgtg cgcgccgttt cggtccgcag ccgctcggcg
    40021 acgatcagcg ctccctcgcg cgccgtttcg ggcatcagca gcgcaaactc ctcgcccccc
    40081 cagcgcgcgg cgatgtccgc cttgcggcag cagcgctcca ggatccgcgc catcgtctgc
    40141 agcacgtcgt cgcccgtgcc gtggccccag gtgtcgttca cgcgcttgaa gtggtccagg
    40201 tccaggatcg ccaccgagag cgcgtgaccg ccccgctcgg cgcggccgat ctcctgttcg
    40261 atccgttcga gcaggatccg ccggttccac aggcccgtca gcgcgtcgcg cgaggcttca
    40321 tggagcagca tcgagacggc cagcgtccgc gcggtcgtct cgcgcagcgt cgccagccag
    40381 gcgggctgct catcccagct cgtcggcgcc gccaccacct ccagcaccat ctcgacgccg
    40441 tcatgccggc gcgtgtgaaa ctccaggctg cgattggcct ccagcggatg ttcgaacggc
    40501 tggccgataa gctgcaccgc gtcgcgcttg aacagctggc cggcggccgc gttggcgaac
    40561 agcacgcgcc ggtcctcgcc gatcaccagc atcgcgtcgg cgctgcgctc gagcagattg
    40621 cggaaattcc tctcggtctg gtgcagccgc tccacgctgc gatgcagcaa ctgatcgacc
    40681 ttgtggcgct caatggcgtg gcggatcgcc cgcgccagca gcagatcctc cgggcgcggg
    40741 tcgaccgccg ccggatcgcc ggcgagatag aattcccgcg gcagccagtc gaacggtccc
    40801 cgcgtccggt ccgccgccga cgccagcacg tcggccagat aaccgaaccg cacgacggga
    40861 atcccggccg gcacaccggc ggtgccatac gtttccgccc cggcgatcct ttcgccggga
    40921 tcgatcagaa tcgcctgcag cttgtcgtgg acatgctgcg ccagctcacg ccggcggcag
    40981 acacccagcc ggcggccggc attcacccgc cgcagcccgg cctcgacatt gcgcgccaac
    41041 gggtcgctgt cggtcaccag cagaagctga gtggaatcgt gtggcacgcc cgcgagcccc
    41101 cgccgccgat tgagggatcg ggtccccctg atccgtatcg gccgcccggc cgtcagggct
    41161 gaaagcggat gtccaaagtg gacgtgttgt tgatccccag gttcacctgg cgcaggaagc
    41221 ccagcgagcc gccgacgcgc tcgtagattc ggatgcggcc gtcgtcgagc gtcacggcgg
    41281 ccaggtcgcc atcgaccgga tcgatcgcga tgctttgaat cacgccgtca tcctcgttct
    41341 cgtagtcggc gtagatgaat cggccggtga gggtcaggtt gtcgccggtc agatcgaaga
    41401 cgcggatttc gccgtcgggc gcggcggcgg cgttgccgac catcaccgtc cggccgtcgg
    41461 gcgcgatcgc cgcatgcagc gcatcggccc cgccgatcgc cgcggggggc gtgacgacgg
    41521 cggtcacgcc gttgggcgtc acgtaaaacg ccgccagatc gccgtcgtcg aacgtcacga
    41581 ccacgcgcga ctggtcgggc gtggaccgga tcgtcagcgg atcggtcccg atcggcgtct
    41641 cgttcaacac gagctcggcg ttgtcgtcgt cgaagtccgt cgtgccgggc cgccgccggt
    41701 aacccaggat gctgtcgcga ttggtcgaga gcacgagcag cagcgaattg ccgatccatg
    41761 tcgcaccgcg cggagaaatg gtgttgccgc cgctcggctc gcccaggttg ctggagccgc
    41821 ccagcagcga acccgaataa gggggaaagt cctcgttgag tgtgatccgg ggcaggttgc
    41881 tggctccgaa gtcactcaag acgatcgcct cgtaatccgg actgacagct acggacagga
    41941 tatttgagcc atagtcggcg gtgtcggaga ccgagggcgt gggcagggtg agatctatgc
    42001 caacgatgct ggtgctgctg ttgccggcga ccggcaccag cgccagcgcg ccttcatgcg
    42061 cgaagtcaaa ctggatcggc ccgtcgtcgg tgtcgatgtc cgggctggga tcgatgttgg
    42121 ccagcagcgc cccggtctcg ggattgatca cgcagatccg gtccgctccg ctcgtcgtcg
    42181 cgatcacgcc gaccacgagc cgcgtcacat acacataatg gcccgtgtgg gacggcgcca
    42241 gatcatcgtc ctcgaccacc gtgatcgcgt tcagcccccg cggaatgccc gccggcacct
    42301 ggcagacgat ctcgctggcc gactggctct tgacccgcgc ccgcttgccc cccaccagca
    42361 cgacggcgtc gctcaggccc gagccgctga cgcgcagcag atcgcccacg accgtcaggc
    42421 cgccggtcac gtcggtgacg ctggtgacct gcgccgcgcc ggcgaacggc gccgacagcg
    42481 ccgtccccgc cacgaaggca tagcccacgc tgacaatccg cgtccgcggc agcagcacat
    42541 tgcccagggt ccccaccgcc acctgcagat agaccggctc cggcgtgttg aatttggccg
    42601 ggtcgagcga gcccaggccg ccctccggcg tgccgctgcc gacctgatgt tcgaagatgc
    42661 cgttgccgtc gggcgtcacg gtcgcgcttt cgcgataaaa ctccgtgccg gaattggccg
    42721 tcgtggcgcc tcccgccgag ccgtcgcgcc acagcgagaa atagagcgtc tgcgccgtcg
    42781 tgatcggcgc gcccccgctg ttggtcagcc gcccctggta gtgcagccgt tcgggcggcg
    42841 gcgccgccat cacgccgggg atcacccacg cggccagcag ccacgccgcg ccaaatcgga
    42901 tcagtcgcct gctttgcatc gtccgccgcc gtttcttccg tggaaattcg gtatcgggat
    42961 cgagatcggt atcggcatcg ctctcgcctc cgccccctac gggaccgccg ccggtcgatc
    43021 cggatcgagc cggacgagga cgatggcctc ggccgagcgg agcggctggc gcggcgccgc
    43081 cggctcagtc caaacccggt tcccccgccg cgtcagctcc agccaggccg ggtcggcggc
    43141 gttgcttccg atctgagccg ccagcggtgg ataaaacccc accccgatcc ggtgcgacgc
    43201 cgacgccgat cgccccgcgt ggtcgccggg gagtccatag ccgaacagcg cgccgtggag
    43261 gcgcacgccg ccgccggtat ggcccgcgcc gccgcccgtc atcgtcgcga tcgtcgccgc
    43321 gtcggccgcc gcccgcgtcg ggacgagcgc aaccgccagc gccagtgcga cggctggcag
    43381 ccgccacccc gaccgagcgc ctggcccggt ccgcttgctc gtccgatgga agaggcacct
    43441 catctcgcgc acctttcgca acgcatccgc ctggccgtcg acgcctcagt ctagacccgc
    43501 gccccggcgc agacaacgaa aacgacgcgc ggattcgggg tttcgcctcc gcgccgcccg
    43561 atcgcgcttg caaacagccg gggccattgc caaattcatc tcgggcgatg gttcccccgt
    43621 tcaaatcgtt ccggatgacc cttcctgaac gcccgcgact actcgccaat tcaccccagg
    43681 agagacaaag actgtgcctc aggcaaacgc cgatcacccg tgccccgatg ccgacctgtg
    43741 gattctggcc ggccagagca acatgcaggg ctgcggccag ttcacgcccg aacgcgccgc
    43801 ccagcccgac atggccgagc atccccggat tttcgccttc gactcgctct atgaatgggt
    43861 cgtcgcccgc gaaccgctcc accatctcta cgcggccgtc gatccggtgc atgtcaacct
    43921 gatgcgccaa tcgccctata ccgacctgac cacgctggac gatctcgtgg cgggcgggcg
    43981 cgccagcacc gcccgggccg ccggccccgg gatgttcttc gccaggcacc tgctggcgca
    44041 tctgcccggc gaccgcaaga tcgcgctgct gccggtggcg cacggcggca cgacgctggc
    44101 ccagtggtcg cccgacctgc cgggcgcgcc cggaaccacc ctctattcgg ccatgctcaa
    44161 gcggctggag gccagcggac tgcggccgcg cggcgtgctc tggtatcagg gcgagtccga
    44221 cgcggccgag gactcatcgg cctcgactta cgccgcgcgg ttcaaagcct ttgtcgaacg
    44281 cctgcgccgc gataccggcg tgcccgatct ccccatcctg acggtccaga tcggccgcgt
    44341 cgccgcctgg gaacgcgccg attgctggga acgggtgcgc gaggagcaac gcctcgccgc
    44401 gcgcgaaatt ccccactgct atctgacgca cgccatcgag ggcgagctgt gcgatccgat
    44461 ccacctgtcg gtcgaggcgc agagcgtcct gggtcgccgc ctggcgaaca tcgccctgac
    44521 cgaagtctat cgcgtcggcg atttcggccg cgggatcgag ctggattcag ttcaggtcga
    44581 acgcgatccg ctcggcgggc ggttcctgat ccgctaccag ggcgtcaccg gcgcgctccg
    44641 ggcggtcggc cggccgtcgg ggttctttgt gcgctcgacc caggcggggc tgacgagcat
    44701 cgccccctgg cgcgtcgagc tcaatgcggc gggacccggc acggtcctcg tgcatgcgcc
    44761 gatcggctcg cccgaagagc aggccgcgat cggctacggc gagggcctcg accccctctg
    44821 caacatcttc gatgaagccg acatgccgct gccgtgcttc gggccgcttt cattcgattg
    44881 cccgccggca ctctagccga cgcgttcctc tcgcctggct cggcgcgatt gcctttggcc
    44941 cccacggatg ctagattgcc ccctcagatt ccaacggcaa gggaagggtc cgacatgacg
    45001 agctgcgtat cgcgggcgat tcgccgaatg ctcctgctgg cgtgcctggg cggcgtcctg
    45061 acggcggttg cgtgcacccg cgtccatagc ggcgcaccga tctactccgc caccgtgtcc
    45121 gcgcagtatg acgaggaggg caaggtcgga ctgacgcggc gccaggcgct gcagtcggcc
    45181 gttcgcgaag cgcgcaatca tattttgaac aaggcactcg agctgcgcct gagcaatggt
    45241 caagtgctcg gcgatctggc cgtcgtcgat ccgtttgtcc gcgccgtgct ggaggatacc
    45301 gttcgcgccg cccgcatcac cgaccgcgcc tacagcgagg acggcctcgt caccgtcacc
    45361 gtcagcatgg aactggaacc gctctacaag ctgatcgagg aatatccgca acacgccctg
    45421 cagtaacgct cctccccagg acatacgtcc ctcgtcctga cttatccccc ctccaggcta
    45481 cgcagccgct cctggatcgc ctcccgatct gcggcttcgg ggctgtccag atagattcgg
    45541 aattgctcgg cggcttcggc ctcgcggccc tggagttgat acagattccc cagattccgg
    45601 tggacggacg cgagggcggg cttcagctcc agcgcgcggc gcagcgccgc ctccgcctcg
    45661 gcccgccgcg cgggcagaaa cgtcgcattc aacccgagcc agacccagcc catctcctcc
    45721 ccgggcagca gccgcgtcgc gatgcgcgcc tccgccgcgc cgcgctcaaa ctcccgcgtc
    45781 tgatacgcct cgcgcgccag attcacatgc ccgcgccagc ttgcggggaa gcgcgccacg
    45841 gtccactcgg cgtaggccgc ctggctgcgc cacgcgccgg cgtaatgcca gctcgcggcg
    45901 cacagcaaca ccacgatcag cgccaggccg tagccgaaca gccgcggcag ccgccgctcg
    45961 gctcgaccca atcccgccgc cgccagccat ccgagcccga gacacccggc cgccgagggc
    46021 cagtaccaca gccgctcggc ggtgatgacg ccgatcggaa aggccgcgtt cgaggtcagc
    46081 ccccaggccg ccaggccgcc gagcacgagc cacgcggcgc cccggccgct cccttgccgc
    46141 gcgcagcgga gcgctccgcc gacgaggccg gccaggatga gcaaaccgat ccacgtccag
    46201 ccccagcccc agccgcgctc gatcggcagc gaataggcgg agtaatccgg cgacaacccc
    46261 aggggataga ccaggcgcga cgccgcgtaa ccgaaaatac ccaggacggc cggccaacgc
    46321 gccgcgaatg tcgcccgcgc cagcggattt tcgtggaatg ggatctcgcc cggcgccgcg
    46381 gccaatccgc cggtgatccg ggcgcgcagc accagatagg ttccacccac cgccagcaac
    46441 gccagcgtca gaagcgcccg ccgccggccg atgatcccgc gcgagccgcc ggccggatgc
    46501 gttccgcccc agccgcgggc cgctgccgcg agccccagat acactgggta aaggatcgca
    46561 tgatccttgg acccgagcgc cagcgcatag cacagcggct gttgccagag cgcgtgcgcc
    46621 gaccccggcc gccgggcatg aagccacagg cccagcagca tccacccggc ggcgagcgtt
    46681 tcggcgcggc cgaccacctg atggaccgcc tcggcgtgga tcggatgcgc cgcgaagatc
    46741 agcgccccgg ccagcgccgc ccgccgcggc atgccgcagg cccgcgcggc gcccagcagc
    46801 agcaccgata ccgcgccgtg cagcagcagg ttgagcgcgt aatacggcca agtcgtctcg
    46861 cccgccgccg ccttctgcgc caggatcgtc aggcgggtca gcggacgcca ctcatgcttc
    46921 agccggtcga ggggccacca cggctgcagc ggcacgcgca gccagtcgcc cgcgccggcc
    46981 ggcagcggcc acgcgccgac ctgttcggca tcgtcatgcg cgaacgagtt gaacagcgcc
    47041 ggctcgaacg ccgccaatgc cgcgacaaag atcagcaggg cgcttaacgc cgccgcgcgc
    47101 cgcgttgccg gaccgccagg tgcgtcgagt gagccatcgt tcatgggcac gtttgtggca
    47161 catgcccgcc cgatcctcaa gcggcgatcc ggtgaaccgc gtccgcgcgc ccggctgtgc
    47221 tatcattttc attggacgaa ccgcaccgcg aagaccggac cgcgcgatga acacgaagca
    47281 gatggcaaaa ctcgggatcc cgttcggccc gccgatgcgc gaggcggtgc acgcagtcgc
    47341 ggccgccgcg caggccggcc tgaaaaagaa gagcgacctg gccgcggccg tcgcggcggt
    47401 cgtcgcgcga ccggaggact atgcggccga tgggttgttc ggcgcgctgg cgcgcgaact
    47461 gctcgccgcc cgggcggcgc cgccgccgtt caacgaccga cccgagccgg ccccctggcg
    47521 ccaatggggg accgacctgg accccctatc gatcggccag atgcagcagg cctgccggtt
    47581 gccggtggcg gtcgccggcg cgctgatgcc cgacgcccac gtcggctacg gcctgcccat
    47641 cggcggcgtg ctggcgacgc gcaacgccgt catcccctac gccgtcggcg tcgacattgc
    47701 ctgccggatg aagctgacgg tcctcgatct gccccctgca gcgctggatg ggcagaccga
    47761 ccggctgaag aacgccttgc tcgcggaaac caaattcggc gtgggcgcgg cgttccgtga
    47821 ccgccgccag caccccgtca tggatgagga ctggagcttc acccggctgg cccgcgagtt
    47881 gaaggacaag gcgtgggcgc aactgggcac cagcggcagc ggcaatcatt ttgttgagtt
    47941 cggcatcctg acgctccccc aggccgacct cggcctcgac gccggaacct atctggcgct
    48001 gctctcgcac agcggcagcc gcggcgccgg tgcgcaattc gccacgcact acagccgccg
    48061 cgcgatggag ctccatccgg aactacccgg cgaactgcgc catctggcct ggctcgactt
    48121 ggaccgcgag gagggacagg aatactggcg cgcgatggaa ctgatggggc gctacgccgc
    48181 cgccaatcac gaactgatcc atcgccgcgt cgcccgccac ctgggggcgc gcgtcctgct
    48241 ggacctggaa aatcaccaca acttcgcctg gcgcgagcgc cacggcgaag cggacgtcat
    48301 cgtccaccgc aagggggcga cgccggcggg cgcgggcgtg ctggggatca tccccggttc
    48361 gatggccgcg ccgggctaca tcgtgcgcgg ccgcggcgcg gccgaagccc tgaactcggc
    48421 cgcccacggc gccggccgcc gcatgagtcg caagcaggcc gtccaaagct ttacctggag
    48481 cgacgtgagg cgctacctcg ctgaacgcaa tgtaactctg ctgtcggcag ggctcgatga
    48541 agtccccatg gcctacaagg acatcgatgc ggtgatggcc gcgcagcgcg atctggtcac
    48601 gccggtggcg cgcttcgatc cccggctggt ccggatggcg cccgccggcg aacggcccga
    48661 ggactgatcc ggtcctacgt ccaactccgt ctctcgtccc tcgtcctcgt cctcgcttct
    48721 cgcagcctcc gccgcgaggt agcgggttca cgcgtaacgg aacggctgca agtcctcgcc
    48781 ggcggcatgg cagcggacgc cggccgccgc gagcacgggg tcgcgcgcga gccgctcggc
    48841 cagcggccgc gcaatcagga cttccgattc gaaatggccg acctcgatca ccgtcaggcc
    48901 gcgggcatgt gcctcgatcg cctcgtggta tttgatttcg ccggtcagga aaatctcggc
    48961 ctggccggcg gccgcgccga gcagggaacc gccggccccc gtgcacagcg ccaggcgttc
    49021 gacgcggtcg cgctccggcc cggagacccg cacgaatctg agattcaagc tcttacgaac
    49081 atgttcgata aatgttgcga agcggaccgg ccgccgcggg cgcgctacca ggcccagccc
    49141 gcccacctcg ccgcttgccg gcgggaacaa cgggcgctcc acctccagcg ccaggcgttc
    49201 ggccagcgcg tggttcgtgc tccaggcggc cgcgtccagg ttggtgtgcg ccgcgatcag
    49261 cgccatgccc gaccgcacca gcccggccac cagtaggccg ctcggggaat cgagctcaag
    49321 cgctttgagc ggcttgaaga ggagcggatg atgcgccacg atcacgtcgg ccttgagcct
    49381 tcgcgcctcc gacagcgtgg gcggcgtcag ctcgaggcac gtcatcaccc gccgcaccgg
    49441 atcgcgccgc cgccccacct gcaggccgac gttgtcccac tcggcggcca aagccgccgg
    49501 cgcgatccgt tcgaccgccg ccatcacgtc catcacccgc gccgcgcgtt tgcccatccg
    49561 cacccttccc ccgaattcgt cgctcgtctc tcgtcctcgt gatcgacccc ggagctacat
    49621 tatggcaccc tcccgcgtcc gcgctacctt cattgcatct ctggtccgtt gtgagggttt
    49681 tcggccctgc tccgttattc cgttgacgac gccggtccga tagcatatcc aatggagaga
    49741 gcgattcggc ctcgggggta cccccgggag tgaacagcga tcaagccgga ccgcgtcggc
    49801 tccgttaaag ggagcaggaa aggacccgcg cacctgatgg gttatcacga cggcgaggca
    49861 cagtacccct tggaagtgtt cctgatctcc ggcgacgcgg aacacgaggc cttcctggag
    49921 gccgacatga gcatgaacac ggaggggcgc ggcacgcccg tgcacgacct ggccggtctt
    49981 gagtcggtcc atgacgacct gcgcgcgcgg acgctcgtcc ggctgcaata tgaccggttc
    50041 tcacccggca tcgcgcgccg ccgcggccat taagccccta catctgaaat tcgtccctcg
    50101 tccccgtcct tcgccctcgt gatggccgca aaggcgtatc ccgggaattt tctcccacgt
    50161 catgagccat ccgttcacga ggaacgcgtg atcggccctc gacgcaccat tccgctgtcc
    50221 actttgctgg gaaccgccgc atgaagcgtc gcgccgtgat cctgctggcc atcgccggcg
    50281 tggtgctggc cggcgggtgg tggttcgcgc gacccggtcg agttcgcaac agcccgccgc
    50341 cccccggccc gatcgtcgcc ttcggcgaca gcctgacggc gggaaccggc gcgccgcgcg
    50401 gcacgagcta tcccgatgaa ctggcccgcc tgatcgggcg cccggtgctc aacctgggcg
    50461 tcccgggcga gacgatcgcc gacgcggccc gccgggtcga tgatcaactg ctcgcgctcc
    50521 ggcccggcat cgtgatcgtc ctgctcggcg gtaacgacgt gctccgccgg cagaacctgg
    50581 acgagtcgtt cgacgagttg gaacggctgg tccgccgcgt gcaggaagcc ggcgcgatgg
    50641 tcgtcgtcgt cgggctgcgc ggcgtgacgc cggtcggcgg cctcggcgcc cgctatcgcc
    50701 gcctggcgcg ccagacgcag gcgctgtatg tccccaacat cctgggcggc gtcttcagca
    50761 agcgcgaact gatggccgat ggcgtgcatc ccaacgccgc cggctaccgg gtgatcgcgc
    50821 aacgcatcgc cgatgcgctc cggccgcatc tgggcgattt taacgagccc tgaaaaggcg
    50881 aatcacgagt cgctgaagag cccgggctca cagccaggga cacaaagggc gggatttcga
    50941 gagtcccgag gacgaaggac gagagccgat agccgagttc atggcctgag gcgggtcgct
    51001 ggtggcgatt acgagggcga ggacgaggac gagggacgat gcgcctccaa tcgtccctcc
    51061 gctacaaaac ggtcactctt cgctggggat cgccccgtaa tatttctcgg cgtactcggc
    51121 aaccatccgg tcggtgttga atcgcgggat cagcgtcacc atcgcccggc gaatccgccg
    51181 aatccattgg cgcggaatct tcatcccgtc gcggtcgtaa tacatgggga tcagctcctg
    51241 ctccagcacg cggtagaggt tctgcatgtc ctcggcgtcc tgctgttcga cgtcgggcgc
    51301 atggcgatcc tcgccgatgg cgaagccgtt ggtgccgtca tacgcctcgc gccaccagcc
    51361 gtccaggatg ctcaggttca ggccgccatg gacggcgatt ttcatgccgc tggtgccgct
    51421 cgcctccagc ggccgccgcg ggttgttcag ccagacgtcg caccccgaga tcaggtagcg
    51481 cgccacctgg atgtcgtagt tctcgaggaa gatcaccttg cccagaaacc gcgggtcatt
    51541 ggcgatttgc cacagtttct ggatcaactg cttgccgttg tcgtcgcgcg ggtgcgcctt
    51601 gccggcgaag atcacctgca ccggccggcg cgggtcgttc agcagcgccg cggcgcggtc
    51661 gatgtcgccg aacagcagcg tcgcgcgctt gtaggtggcg aaccgccgcg aaaacccgat
    51721 cgtcagcgca tcgggcatca gcacgtgctg gtaatggcgc gagactggcg tgccgatgcg
    51781 cagcatctga tcgcgcagcc gcacgcggat cgcctcgatc atctggcgct tgagccggaa
    51841 acgcagcgac cagatcgctt cgtcgctcag caccctggga tcggccacct tctcccagaa
    51901 gtcggcctgc ttgaggtgtt tccgccattc cgcccccagg ttctgttccc agaactcata
    51961 cgtcacgcgg ttcatccagc cctgcacatg gacggcgttg gtgatgtgac cgatgggcac
    52021 gtcctcctgc cgcgtggcgc cgtagagctc gcgccacatc tcgcggctga cccggccgtt
    52081 cagctcgctg acggcgttgg cgtggcgcgc atggcgcagc gccagcaccg tcatgcagaa
    52141 cggttcatgc gtgtcgcccg gccgcacccg gcccagatcc atgaactgct ccattgtcag
    52201 ccccagatcc tcgggccaat ggggaatcag atggtgcagc agatcggcgc tgaagcggtc
    52261 gtggccggcc ggcaccggcg tatgcgtggt gaagaccgcc ttctcgcgca ccagttcgcg
    52321 cgccttctcc agcggcacgc cggccttgac gtgctcgcgc atcagctcca gcagcagaaa
    52381 cgcggagtgc ccctcgttca tatggtacac gcccggatcg atccccaacg cgcgcagcag
    52441 gcgcaccccg ccgacgccca tcatcaattc ctggcagacg cgcgtggtct ggtcgccgcc
    52501 gtagacgcgc gcggtgatct cgcgccagtg caggtcgttt tcgggccgca gcgtgtccag
    52561 catgtagagc gtgcagcgcc ccacctgcag ccgccaggcg tgataagtaa tgtccgactg
    52621 cgtcagccgg atcttgcaaa cgaccggctg gccgttggcg tcggtgacga gctccaccgg
    52681 cagatgatgc gggtcgagcg ccggatacga ttcctcctgc cacccctcgc cgttgatgtg
    52741 ctgctgaaag tagccgttgc ggtagaacag cgtgatgccg atgaacggga tgccgaggtc
    52801 cgaggctgat ttgatgtgat cgcccgagag gatgcccagg ccgcccgagt agaccggcag
    52861 gctctcgtgc atgccgaatt ccgcgctgaa ataggcgacc gggtgctgct cgaagccggg
    52921 gcagtgcttc gccccccacg tgttctcgtc ctccagatag ctctcgaatt cgtcgatcac
    52981 ccggcgcgcc agcgtctgga ttttcttgct gtagagcgtc gccaccagtt catcggtgct
    53041 cagcgccttg atcaccgcga tcgcattgtg attcgacgtg atccaggctt cctccgacag
    53101 cgccttgaac aggtcctgcg cccggggatt ccaggtccac cagatgttgt aagccagctt
    53161 gcggatgccc tgggcgaggc gcagcgcggt ttcatgggtt tccagcgacg ggttggcgac
    53221 aggcgtcacg ttcgggtcct ttccagatgc cccgcgcgga gcggccgcgc gggcggaggg
    53281 gtcggtctgg gcatggcttc catcacccca cgcgtgacgg gagttggagt ccccgccagt
    53341 ctacgaaagc ggatgaacgc aatccaaagt ttttccgagc ggggcaggac ttatgagagg
    53401 accgtcgcac agcgtagggc gtgcagtggt tgcgatgcgt tctccgcatg cctggttcga
    53461 tcccgatagc gatcccgata ccgaccccga ttcaccgggc ttttcgccga aacaggttgc
    53521 aatgcccacg gtcgcgggca gaatgacggg gtgccgcgga accgccgcaa ccaacccgac
    53581 ccgcgaatcg cttcggcggc gggccgccgg ccgatcgcct cgccatcgcg cgcgcaccgg
    53641 gacacgcccc gatgatcgtt cgttttttca agcgcaatag tatcctgatc ggtttctcac
    53701 tgatctacct gctgctcgtc gtcctggcga tggtcgagct ggaccgccag tactaccagc
    53761 agcgcaagcg cacctacatc accgaacgct acttggtcac gtcgatcaat caggaaatcg
    53821 gcggcgagaa cctcggcgat ctggaacagg tggcgctcga ggcgctgcgg acgaacaacc
    53881 cgcaggcgat gcagcgcctg cgcgaacaga tctggaagat cctgcgcgcc gacaactttg
    53941 tctaccgcat cgcgatccgc cccggcgacg atcaggaggt cgagggccag ccgctcaatt
    54001 tcatcgtcga tctcccctcg cagaaggaga tcgaggagaa gatcgcggcg cacaattcgt
    54061 tcgccaattc gcttttcctg cgcgacttct ccggccatct ctccacgccg atcgtcgccg
    54121 gcgggcgcca gttcggccgc ctctatgcgt tctacacctc gcccatcggc aacccggaga
    54181 tcgaggaaat cacgcggacc taccgctgga tcgcgctctg gatctggctg ctgatcaccg
    54241 tgctggcggt cggcctggcg cgcagcctgc tcctgccgct gcgcaacgtg atgagctcgc
    54301 tggaggcttc gacgccgcag cgcacgcact tcattccgcg tccgcgcacg cggctcgagt
    54361 cactctataa ccggatggcg ctcgatgcgg tcatcgcgcg catgcaggga cagttgcgcg
    54421 aggagatcgc ccgccggccg caaatgaccg gctgggaagt cgttcacttc atcgtcgaag
    54481 ccttccgcga ccaggtcggg ctggcccttg tcgcctgcct cgagatggtc gccgaggggc
    54541 ccggcgcgat ccgcgccacc ggccagcgcg tgatcgccgg cccgcgcgac tgggccaagc
    54601 atcagcccga actggtccag ttgatcgacg acgcgatgcc ccgcgacgga cggatccagg
    54661 cctcgctgct cctggatgag atcggcctgc gcggcgaggg actggtccat ctggtcgccg
    54721 atcccgagcg gaccggcatc cgctatctgc tcgccctcgc cctggagcgc catctggatc
    54781 atgccgcgca cggctcgctg caaaacatgc tcgagcggct gaccgagctg atcgaggcgg
    54841 gcctgcagac gctcacgctg cgcaaccgcc tgctggtgca ggagcgcggg cgcgccaaca
    54901 tcagtctctc gcgcaacctg ggccacgacc tgaccaacat catcgccacc agcaaactgg
    54961 aactgatggc gctcgaccgc ctgctcaagg ggggccaact gccggacgac gaccggcgcc
    55021 gccagatcct ggtcgaatcg ctccatggcc tgctccgctc ggtcaagttc atgcaggaaa
    55081 cggtcaacct ctaccggtcc tacgccttcc tgcagcaccc ggtcctcgag gtgcaggacg
    55141 gcaaccaact gatcggcgaa tcgatggagc tgttcgaaat ctcgatctcg gccaaggtgc
    55201 gcctggaaca ggagctggcc cccgacgcgc cgcgctgcgt cgtcgatccc cgcctgatca
    55261 agctggcgct gttcaatctc ttttccaatg cgctggaagc gatccgcaag aaggaccccg
    55321 agcgcaccgc ggccggctgc atccgcgtcg tcacgcgccg atcggccgag ggcggactct
    55381 gcgtcgccgt cgaggatacc ggcaccggga tcctcaacga acgcaatgag ccggcccagc
    55441 cgcacgagat cgaaaagatc ttcgagctcg gctataccag cttccggctg ggcggatcgc
    55501 acggcgaagg cctgggcctg aactgggtgc gcacgatcgt ccaggacctc cacggcggtt
    55561 cgatcttcgc cgaaaacatc gagggggcgg gcgcccgctt cgtcattgtc tttccccccg
    55621 tcgaacaggc ccccgacctg gaaaacaccg aaatgcacgc cgcgcgctat atgcgtcaga
    55681 tccaggggct cgtcgaggaa caggagcaat cggcccgtca cggttgaccg tgcgggcggc
    55741 tccctttcaa ccggctccga atcaacccgt aggcgaggat cacggccgcg agaatcgaga
    55801 tccagcggcc cggcagcagc ccgggcggca ggaactcgaa gatgacttcg tgcgacccgg
    55861 cgggcacggc cgtgaccaga aagacgcccg acagccgctg cacgggcgcc ggcgcgccgt
    55921 cgagccgcac cccccaaccg ggccaggcgc tttgcgccag gcagagcagc gccggccgcg
    55981 gaaagtcgac ccggtagccg aaccgattgg gcgcggcctc cgccggatcg tcgagcggct
    56041 cgacgcgata gctcgcatcg atttccggcg tcagccgctc gcccagccag agcacgccgg
    56101 gcgtggtcac gatcgtggcc atcgggtcaa tccccgcggt cgccgtcgtc gccggatcgg
    56161 cggcgggggt ggccagcccg gccgaatgga cgagcggcgt gatccagtcc tcctcgtcgc
    56221 gctggttgac gaggcgcggc atcggcgcgg cgaaggccat cccgcgaaac cacgtgttgc
    56281 gccacagctc cagatccccc tccggaaatt caagccggtc ggccagttcc agtcccggcg
    56341 tccccgacag atcgatcgcg gcgccggcgc cgtaggtaag aatcgtatcc acccccatca
    56401 gcgtcagcca ggccgggcgc gcaatgaccg ggatgagcat gaacccgcgc ggattctgct
    56461 cgggcgggaa ccccgccagc aggttggacc agacgccgat ccagcgcgca ttgaccggat
    56521 catagccgcg ggcctcgtcc aggccataca tcagcaggcg gttggggaag acctcgggct
    56581 ggttttgatc gaacatccac gcgtggacat tgtcgagcca caggatgcgt cccccctgat
    56641 ccagctcagt cagcttctgg accagcggcg tccgcgggta atacgtcgcc tcgtagcggg
    56701 tgaagtagac gtccttgaga tacggcaggg ccagcagcgc cagttccagc gccgcgagcg
    56761 ccggcaggcc gagggcgggc ccgagccggc gcggggcgga tgccgcgtcc cgccggccgg
    56821 cgacccgcca gagcagcgcc agcgcggcgg ccagcgccac gcttacgcgc agccccatcc
    56881 agccggcatg gcgcgtctcg gccgcgtagg cctcatcaac gcccggctgc cccaggccga
    56941 gcgcgaccgg ggattcggga aagctggccc agacctgatc gcgcagggcg aagctgagcc
    57001 agaccagcgc cagggccacg gccgcgcccc ccgcgagccg ccgccggatc gcggccgggc
    57061 ttcccacccg cagcatcgca tcgagcgcca gcgcggcggc cagcgcctga ccgccgacga
    57121 caaacagcat cagccgcgcc ggcacgcgaa agcggtcgaa tcccggaacg aagcgataaa
    57181 acgcggcgaa cagcggcgag aattcgccga gcgccagcgc cagcccgaac aggacggcca
    57241 ccacggcc
//
LOCUS       DAOPCO010000003        56166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_659_length_56166_cov_5.881431, whole genome shotgun sequence.
ACCESSION   DAOPCO010000003 DAOPCO010000000
VERSION     DAOPCO010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 56166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56166
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_56166_cov_5.881431"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="PLS90_00510"
     CDS             complement(<1..1591)
                     /locus_tag="PLS90_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK00913.1"
                     /translation="MSVRAQSRRLILLSALVLAAIGGTAAAQGPASKISGQILYHKQI
                     KESLQEQGVSPSSQEAALLEQGIAPGPLGAEECALYVERPLTQEEKAELAARGITVHQ
                     TYVPPVPDKHPYGFYLSTVAYDALDEVQADERIVLLTSTARLDQPMNDLGLALMNVDD
                     VHNGIGVTARDGTGVNIAVADSSLDTTHPDIPAPVEAYDMTDGTDVGTWGTDVSSTVS
                     DHGTHVTGSALGRGTQSGGQYKGAAPGANLYFYKIGNDFTASASIADEVEAIVRAGEV
                     GCDIFTMSYGGVGSFNDGSEEMEQAIDAAVAGLNPGQVKPMVVFMSAGNEANKKHHAS
                     VSVAPGATSSTFGLTINNSAGVGTYTTALGIRVVWIDDTPGDFNISLNCTNLTGTEDF
                     LLGTSDSSVRGTESRNYGLFPEIAAGATKSYTFELTNSAGSGSTPLVHLFALTSSGLV
                     TFDSPDESYTIGSPGLADDAIAVGSWTSRKAWTDYKGDGWFYNSLNEGTRSEFSSLGP
                     RVDGLMKPDVMSTGAAIISCRD"
     gene            1915..5202
                     /locus_tag="PLS90_00515"
     CDS             1915..5202
                     /locus_tag="PLS90_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK00914.1"
                     /translation="MILLELHRWGGSLRRILLTAWSLALLAACGPPPPALSENEPIAI
                     AEYEPQQTLAGETFAEVPGGLSTVTIRAQGATSDTIVRLNGTALASRVDAYGIIKAFI
                     PAEHLQQPGRLALSLYDEPTSRVSEPVYLEVEGIVVERYGPTEITAGVDFNPQPSGNS
                     GIWLQARNAARGTVLLANGEPLKTAVTDSGLVTAELPPRYYDAPGTLTLTLVDRDADL
                     RSADLHIPILGVRVVDYGPRRTDPGVAFNPQPDGASALWFNLAAPLWTAVVSWDGREL
                     QTSVGADGVVSALVPAELIARPGFHRLQIVDSAGGMKSEPMVFQVGDVAPAAATPVPL
                     APALDSIQIYVKRDGMVRLAGFDLASRGLDLQATRGDQLNLHLRGEPVPIVVEGLSPS
                     GTLAPDSTILFWGEFPRGEQTLHDLHAAENVYLLRWDGGRPPVRYTMSDPLSLPAPDE
                     PILTSCPRTAHLEEDREANFLQHYKGFPTDRVMWATFMAPPREGHRLTLGTLPDLAEG
                     AAPSAIRIMLWGNSRLGIHPDHRWRLALNGHDLGEAVWDDRTHVVHGAPIPERAFSRH
                     EPMMAAGSEVELTRDNNLELRNMQAGETIDSISIDWVEIDYEAVLRPIDDRLTYQFTT
                     GTLAQAVSIENAFSGAPRIFSIDEGIELALPAGSPPDAIRYRYEVKPDSAVTRPRFVA
                     VGPRGYLAPERIESGFASDLKSAQPPEYLVITHARFVEALQPLIEHRRRQGLSTLLVD
                     VQDIYDEYSDSLFSPEAIRRFLDDLFVKEGQGAGDLQYVFLVGDANYDYHNVQKTVPN
                     LVPTYHIESERSVGVPIFALDEYYVYGPEGTTPRVAIGRLPASRPAQVTDYVEKLVAF
                     EDANREEAVAAMEGPRRALLIAGTGFANYCDQVAEKLGEPWEITRVYAERDVARNEAL
                     RGQIVAALNGGCDLVYFAGHGSYWEWRTGAEDMASQTDMLTNAHVRELTNAGRYPLIF
                     SATCYSSLFDSPGYAHPTNNEWVDSGIGTYLVEAPGRGGIACIGHVGESLVLASHKFI
                     LRMIESMREQNVVRLGDAFLAAKRESVGVPYLGITLIGDPATWLGVRFSQGVGPAEPA
                     PAEPAGA"
     gene            complement(5230..6558)
                     /locus_tag="PLS90_00520"
     CDS             complement(5230..6558)
                     /locus_tag="PLS90_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK00915.1"
                     /translation="MILPVRFLHLFRSLRHRNFRLFAIGHGLSLIGTWMQMTAVAWLV
                     WRLDHSAMLLGVVGFAARIPTFLLAPIGGVLADRFNQQRLIIATQALSLVQALALAGL
                     MFSGHIQVWHVIVLSLVLGVIQAVDIPTRQAFLIHMIDDPHDLHNAIGLNSSMVNGAR
                     LVGPSVAGVLIALWGEAVCFLINGLSYFAVIASLLLMRVPGRQRVAGGPALLAHLAEG
                     FRYAFGFAPIRALLLLLALVSLVGSSYGQLLPIFAERILGGDSRTQGFLISGAAVGAL
                     IGALHLASRPSVRGLGRVIAWSPAVMGGGLIVLGLSSWFWLTLAAMPVIGLGMMLQMA
                     STNTVLQTIVDDDKRGRVMSFYSMSFMGMVPLGSLLAGAVAQLVGARAMVICGGACCI
                     LGALGFATRLPDLRRLIRPIYIQKGIIPSPAEPAGLGVAEAPAPAPTQSS"
     gene            6813..7238
                     /locus_tag="PLS90_00525"
     CDS             6813..7238
                     /locus_tag="PLS90_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00916.1"
                     /translation="MRQSTGLAVMVGLVIFSYLAVVSIIPVHRSRQFNQRERITQRLM
                     RIEMAKQEVAMQRDLRPGARLTMEELVAGSQYLDQAPASPSGTYEPGALGELPRFVPH
                     EGEPIVPEPVSPRQPAPAQPGDAGEDDSAAPGESADGNA"
     gene            7238..8497
                     /locus_tag="PLS90_00530"
     CDS             7238..8497
                     /locus_tag="PLS90_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK00917.1"
                     /translation="MASAPTVAPALKPAGLSRAPARRSGEAVVGPRIGFDAASVVGPR
                     TGIGHATAELLTALAEIWPENWPVARVWVNSRRHALPEHDPWMSARVYDVKRTTLPGR
                     LILRGWQYLGWPPAEIWTGPIDLLHSPASYIPAVRAARRVVTVHDIYFKHAPTLVDTY
                     GGKYFYQTFERGLPEVDQIIAVSEFTRAELLKHYRVSPEKVSVVPHAVNLNRFREEPA
                     PDDNEHLRQLGIEKPYLLCVATIEPRKNLVTLLEAYARARRIMEAGGQKLPRLVITGQ
                     PAWGIKPFEARLREAKLQDLVTPTGYVPDAALPALYRQALGFVFPSLYEGFGLPVLEA
                     MACGCPTLISRAGSLPEIAGDAAHYFNPRESGSIARELVTFLADPMHRSALRDAGLRR
                     ARQFTWAATARGTLNVYRRVLGLTECA"
     gene            8606..9709
                     /locus_tag="PLS90_00535"
     CDS             8606..9709
                     /locus_tag="PLS90_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00918.1"
                     /translation="MPMAWAEAGNHILLLEGSPYPNHFMRGDARWGPSLHGQLRRVHP
                     RLHVGAMPPAFPRMLKGGLMGNALRALHRPLDQRRIRRYLRVLGWEPEIVVLMQEPVR
                     YDLLELFPDALRVYYCNDLYGYGHGTANEFREEAECCRRVDVVFTTSDILRRRLLPHN
                     AKTFHIPHAVDLAWWESNRNRHPAEMERIRRPRALFTGALTARIDFALLEEVAGACPH
                     WQFVLIGPYNLAEKEARRLGALGNCHLFGAKPHEEIPGYLALADVLLFPYAGKATAQH
                     CGLPLKFYEYAISGQPILSTDFAQLELPNPDLVHVARSPQQWVDFLTHFERHREQQAV
                     LTRQRRELAERNTYQARIEQQRGILSHLDRASR"
     gene            9706..10689
                     /locus_tag="PLS90_00540"
     CDS             9706..10689
                     /locus_tag="PLS90_00540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00919.1"
                     /translation="MRRVRKPLVSVITPAYNAAQFIAETIASVRTQSHDHWEMLITDD
                     GSSDATVEIVERIAVEDPRVKLLRLARHTGLAAKARNHSMQMASGDYLAFLDADDVWE
                     PRKLEQQAAYLESHPEVDAVCSWYYVFGDPARVQRYREMMWRFETTEVTVDQILMQSL
                     FTSTVMMRARCCEELGGMDESPALSSGQDYEYFVRLVARYRVHRLCEELSGYRLAPAG
                     GSLSTKGQRLERRRQREEAILEALAGKQVLTPALLRRRRAIMHYNLARDCLFELDQPY
                     RGFLWRAVRTWAAPPKAYVMFLLSFLPARQLRCVLTKLLALRQSRLFTGRK"
     gene            complement(10707..11771)
                     /locus_tag="PLS90_00545"
     CDS             complement(10707..11771)
                     /locus_tag="PLS90_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00920.1"
                     /translation="MDNPRVSIIIPVKNRAHLMPALVDSLVHQSLLSWEALVVDDGSE
                     ADELLKISQLCSRDARIRLIRREAGKSGAGACRNLGFRHARGEIIMFIDSDDCISTPD
                     CLADRVEFLGQRGNLDFCVSPVEIFESVPGDLARLHNIVTAETDLIRFLKRDIPWQTA
                     GPTWRRKTLDRLGPWNEDLPSWQDWEFHTRALLAGLSYERFPGGRAYWRLPSAAGSIG
                     AAARSVEHLKQVESLLIETAQRIDKNHSMDRKTRRALAFSFFFTAVEFIALDRPTDAH
                     LLWRQFKQVSHAPQWEIAALELYSRVFKWPGISGACRCLLHLLLPPTDIPQYSTTFRR
                     CGIEGTSTWNAAGDDSSHRL"
     gene            complement(11777..12694)
                     /locus_tag="PLS90_00550"
     CDS             complement(11777..12694)
                     /locus_tag="PLS90_00550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK00921.1"
                     /translation="MKTTDTISIAICTRNRAALLKPCLDALTIALASRPTPVIVVDNA
                     STDETRAIVEGCGSLNIQYVLEPKVGLSRARNTALALCRTEYLVYLDDDAKPLPFWMD
                     AIEEGIARWHPPFFGGPYRPYYLGGKPRWFADSYGSMHYEKQEGPLPPNGFLSGGNMG
                     WRVDLIRSLGGFPEHLGMIDTRIGVGEETYLQLALKERGNNGHFFPRMEILHFMAPEK
                     MSLSYRARRMWQSGLQARATNLGRSRARLRTLLYSMVFVPIHLAGMLFRDRTVDPCWQ
                     GYVYRRILPHLTYWGEFWARLSGQRSKVD"
     gene            complement(12681..14309)
                     /locus_tag="PLS90_00555"
     CDS             complement(12681..14309)
                     /locus_tag="PLS90_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00922.1"
                     /translation="MKPIALYLPFGPVHLRNLLILSRHMPEWDFVICHSTRTESLAAC
                     PAGNGFSWLNLDDIDSYLIAAGQRIKVAIFSKVFPDKEIFLLLAALIRHKLPAIAIEE
                     VNQHAMTEGRINNYIVPIDHLFLASPYEADALTGLGVPAEVVEVTGWPFHSQLLDDGI
                     DHRSETRARLGIQGDEEVITLALSLLSDGAGAVASTETQRVRHDLITTVEAARLPGQR
                     TVVKLHPREDFDAGVRSVKSCSPRVIVAPPDLSIHHVLEISAACINRGNSQVILEALL
                     RSIPVLLVPLGLKTIFDGTNSKEVLSGSEDLRNALLRLRRGIRPDYSAVLANHSPIAP
                     DRAIARVVHRIEEIACEPLTRHPDMLVGILLWAASLQYQDVTESLVPHLSQDSRSDLL
                     QSVKALLRECATHHDIKTILNNVNIGLLSQAVLAIWLRQLSKYGANREDLALLSSIQD
                     FPSDTNSYRYLQHYLLAAELLASGGYRDRAEVILEKVKEYPGLSLRWPDLWRSLCWQM
                     GDYTPRNGYNWMRRKWGRGFRKIRMQRRVLNEDN"
     gene            complement(14306..15292)
                     /locus_tag="PLS90_00560"
     CDS             complement(14306..15292)
                     /locus_tag="PLS90_00560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPK00923.1"
                     /translation="MKLFWANDTRHVCHVGCLAVTDAHDRMIWEAGAVVIQRHYVGQG
                     KEIWNGDENQAIAAALKSRWGRAIFECDAVVVNGEGTIHHGNGNHLLAFLGAAQRLGK
                     RTLLINSLLEDLNAFHDVIRNLDYLVVREPRSAAEARRLGRDACIAPDSIVAAAFLEQ
                     ASADFGGQVVIGDAHPSRADILEVLDRLKRDHPSWDLHHEDRKLDWPHAVANLKTASI
                     YITGRHHGVYLAGLAGIPFVPLPSNSHKIEALIEWSGCDIPVCSSLDQVREAIRLAPF
                     KRDEFARFHHFLTRNRPLPRLPPIDLPLRRERPSLQLRAYWAWATFKNHWLR"
     gene            complement(15289..16638)
                     /locus_tag="PLS90_00565"
     CDS             complement(15289..16638)
                     /locus_tag="PLS90_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK00924.1"
                     /translation="MPDNAEAPAIVRRAETTPGAEAATPWAVRMRDIGKAYRLGETRA
                     LKDNFREELTSLLRRGFGLARRGRGDKGTFWALRDINLEIARGETVGIIGRNGAGKST
                     LLKILSRITAPTTGEMRYRGRLASLLEVGTGFHRELTGRENIYLNGAILGMRRAEIAQ
                     QFDAIVEFAGIEQFLDTPVKFYSSGMYVRLAFSVAAHLRTDIMVVDEVLAVGDAQFQK
                     KCLGKMENAAHDGRTILFVSHNMGAIQNLCRTSIYLDAGAVRAWGRTSEVFDQYIRDT
                     LGGAENGGRVDLTIRNPPTQGPARLLYCELRNRDNELTSVFRYREPLRVTFGLEVLEP
                     LPSLSFHLGVDRLDGTRVYTSDSEENDLHPGTMLPGTYIISFQPRGMHLMPGDYKVSK
                     LMARQGRQSLDLVMDVTRFHVTNVPLEGPPPAVGTGIICGSAHWEVAPTQCPAEKST"
     gene            complement(16640..17497)
                     /locus_tag="PLS90_00570"
     CDS             complement(16640..17497)
                     /locus_tag="PLS90_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK00925.1"
                     /translation="MNPQETVISSDPRTRLIQAFRDLVEYRELLGAFILRNLKLRYKQ
                     TALGIIWVVLQPLITGVVFTAIYGLIRGSFDGAAALLFFMAGLAPWTSFQQGTQMAAT
                     SLEQNANLIAKIYFPRMIVPASQVLSSLLDFLFAFAMLAAIAFLAGAFTASLLLWLIP
                     LLIIQLATAVGLGLFLSILNAQYRDVKYAITFVLQLGMFITVWLPLEAMGRGEFHSVD
                     WGWAVPYLYQVLSLNPMAAVVETYRAVIERSPINSMLLLKAAVMSALVLFFGARFFAA
                     RERRLVDVL"
     gene            complement(17817..19334)
                     /locus_tag="PLS90_00575"
     CDS             complement(17817..19334)
                     /locus_tag="PLS90_00575"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPK00926.1"
                     /translation="MHNLAALLEETRLAPAARVPADLSIEAVTDDSRRCGPGVLFVAI
                     AGGRADGHQFLAQAAAAGAPAALVSSPRAKAPAGLHLIQVENTRLAYARLCQKLAGDP
                     SAGMLAACVTGTNGKTTVTYMLEAILLAAGLKTGVLGTVNYRWGDHVELEPAPQTTPS
                     PAELAEYLRRMRAEGVQALVMETSSHALDQHRVDGIQFRAAGFTNLTQDHLDYHKTMD
                     EYRAAKERLFTEVLPAAGGGVAVLNLDDPAGRHYAERVRQTAPGVQVFSFSFHRPDAD
                     LLVDTVHVTRHGQYVRARLRGRPFYLQLRLHGLYNVQNALVAAGMALGLGLDHDAIAQ
                     GLAATRGAPGRFELVEAGQPFPVIVDYAHTPDSLQQTLLNACAFRTGRLIAVFGAGGD
                     RDPGKRPQMGRAAARLADHIIVTNDNPRTEEPKAIAEMILDGIKEAAFGKTRWQVILD
                     RASAIREAIRLATPADVVVIAGKGHENYQDAMGRKTHFDDREQAAAAVAALRRAK"
     gene            complement(19436..21649)
                     /locus_tag="PLS90_00580"
     CDS             complement(19436..21649)
                     /locus_tag="PLS90_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPK00927.1"
                     /translation="MLRKTYRFRLLLLWCSTFFVFSAILVRLYHVQVIDNEKYLVRAA
                     EQQHKRVTLTPRRGDILDRNGSILATSYFTDTIVLDTRKLRRGKGDEAPLDIPPGLID
                     DLAEVLGRPRSQIQQYFDKPYRYYVYRKAPEEVSQAMAMIDERYDLPPNTIIFEKQSK
                     RYYPKAHLAAHILGFTKIDDAGDNIGLAGVELQYDEYLKGSYQETQIPVNSLRTGLAP
                     IADEVIESTYGHNLILTIDEQIQMFTQRALRRRVGELQARSGVAIVMDVRTGGILALA
                     NCPDFDPNHFSETDDDQRLNRALTDPFEVGSVMKILTTTLLLDGGLLTPSENVNTGSG
                     AVFLHGRWIRDVHAIGGWVPLRHAFAESSNVAMALLSERIEQVAYYQGLRRFGLGSSL
                     GIDLPGEGSGILRPVEQWTAMSRASLAIGYETSLTPLQAVSALAAIGNNGWRMAPHVV
                     QAVVSRDGRTIREFPGEPVERVANPETCANMLELMEAVVLEGTGKGRAEVPGFRVGGK
                     TGTTVKHYRSDSGRKLYIASFAGLFPIDEPRVAIFVTVDEPRGAKYGGEVSAPVFREI
                     ALKVAHILGIPPSDPAEYEAYRRKEEGFVDPALRVAEDGDSSPSAEDNGVLAVDADSG
                     GMPAALPVEPGRPVLPAVDLALAESLLPATGDEDAEAERPRMPDCLGLTMVEAWERLA
                     EAGIEARMIGSGLAVRQDPAPGRRLPASRAAMVVFAQNTDVAQKASAGEDAFQAQ"
     gene            complement(22522..22992)
                     /locus_tag="PLS90_00585"
     CDS             complement(22522..22992)
                     /locus_tag="PLS90_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPK00928.1"
                     /translation="MAGYAQRLDPGDNVRLVPFSALMRVLFALGLLLLFALGHLHLRF
                     MLDGVRRDAIRLQTTHDQLTSENNALRGELEALKQPERLFEFARAELGMMPYGEERVE
                     IRMPEEIYTRYAMARTKAGGPAQPPLSPTAIQAALWLERLGERVGWAPAAQAGE"
     gene            complement(23075..24049)
                     /gene="rsmH"
                     /locus_tag="PLS90_00590"
     CDS             complement(23075..24049)
                     /gene="rsmH"
                     /locus_tag="PLS90_00590"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPK00929.1"
                     /translation="MSEPVDHEPVLQEEVLQTLDPAPGKTVFDLTLGLGGHSRLILAR
                     LGEEGLLVGVERDPQMLAEARRRLEAAGCPRSRYRLLLANHEDLAELIRHDPQLADVP
                     APDAVLLDLGPSTPQLLDPDRGLSWTSPSLDMRMNPRAGGPTAAEIVNSWPPKELARI
                     FREYADERWAGPIAQRIAAERVKGPIESGRRLGEIVAAAIPRKAWPPRIHPATRVFLA
                     LRIEVNGEYRSLQRVLPAAFEALKPGGRLVVITFHGGEDRIVKQFMREKSTAELPPWP
                     LPQGAAQAPARLLTRKPLTPREDELRHNPRSRSSKLRAIEKLGDETAG"
     gene            complement(24057..24584)
                     /locus_tag="PLS90_00595"
     CDS             complement(24057..24584)
                     /locus_tag="PLS90_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00930.1"
                     /translation="METRFLGNEQMTVDGKGRVGIPARFMAVLRAVCPEQTEQVGVMI
                     TPDESIRVMPANYFEQEIERLSQLDDQVDDERMVLNLATSTAELLPLDKQNRIKLNPL
                     MMEFCSIDRQVVFVGSLKYMQVFDVNKWRQMVNAGLKVYGKATTAIAARKAPAAPVNQ
                     FVLNVPGAGPNAPGA"
     gene            25163..26167
                     /locus_tag="PLS90_00600"
     CDS             25163..26167
                     /locus_tag="PLS90_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK00931.1"
                     /translation="MVEPAAVGNSAVPETGAPVMIEVSNLTKLYGLTRAVDNISFTVG
                     KGEILGFLGPNGAGKTTTMRVLTCYTPASAGTARIGGYDVSRQSEQARSILGYLPENA
                     PLYQDMLVDDYLRFMAEVKRYPGSRRRAIVEEAMEECGLTDVRKRIIRNLSKGYRQRV
                     GLAQALLGDPQVLVLDEPTVGLDPRQIAEIRQLIKGMAGRRTVILSTHILPEVSMTCQ
                     KVIIINQGRIEAQGTPESLVSALEAANVVMVTVDGPAAAVQELISRVPGVKSVAQERM
                     LGGSTAVYRVEAAPQGDPRPELAQAIIGGGYRLFELQSSGYSLEDIFLRIISRQKEAA
                     "
     gene            26167..26958
                     /locus_tag="PLS90_00605"
     CDS             26167..26958
                     /locus_tag="PLS90_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK00932.1"
                     /translation="MNFLPIYRRELKTYFQSPSTYIVMALLFLVLGMIYSQIMVLFSN
                     MSAQAGMPNMFGQTPPEPNVTEMVIRSLFQIMIALILFTVPLLSMRLVAEEKSRGTFE
                     LLVTCPISDWAILMGKYLALVTVGVIVILLSGLFPVITWWAGRGSEVVIEWPIVLSCW
                     LGLLLIFAAYAAFGVMASSFSENQITAGVITLIGLIMWNMLASFQIESFPRIQQIFTE
                     LAAEPHTTNFVKGLLTTKDVAFYFLASFLFLFIASKTLDARRWRV"
     gene            26962..28614
                     /locus_tag="PLS90_00610"
     CDS             26962..28614
                     /locus_tag="PLS90_00610"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPK00933.1"
                     /translation="MHDKTLKIWFRTLPVIGVLFVIAGFGWAIRQAAVDPVVYTLVGI
                     GLALFLTLFAKAELANLKYYLHVVVYSLLVFILCAIVYLAIRQYDKQADLTEQNLYTL
                     SDQSRQVLDQLKEPVEIVFFFSTDQPFRDIMRLYGDASDQIQFKVIDPIKDPVAAMRY
                     GENIRTGEVVIRKVPEKGEGTPAPAAETKQKRISATEFGQGFTQMGVQAENALTNAII
                     EVTRAETIKLYFLEGHGEVSYDEAPMPQPGQRSSVASLSALRKFLEDPGMETARLNLI
                     TSGLVPEDATMVVIAGPAGDLLPPEISALRDYLTQRYGKLMVLLDVPQENFALRLPNL
                     LGLLEEYGVSSPDEVIIDLANPFSPVQPIVQDYAEDHPITRDLRQAMMLSISRPVVSR
                     MQGGGQWQVTELMRTSPSSFSKDLQEVVANERITPPPRSEMQPQTLAVAVSPRTPPQM
                     PNMPPLEEPEGARLVVFGTSEFVQNSTLTQSNVAVNLMLNTINWLAEQEDVIAIPPRQ
                     LEGTPIILTPQQLRVIFVLAVILLPGALFFGGVSYSIARRRR"
     gene            28833..30626
                     /locus_tag="PLS90_00615"
     CDS             28833..30626
                     /locus_tag="PLS90_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPK00934.1"
                     /translation="MSWKRTVIVAVIAVLAGAWYVWDQKRIEEKEAVETVASRLVTQA
                     KEAFQSVELERAAAGDVQLVREDETWRLTEPVATRADETAVNSLIDAIDSSRKENTFE
                     VADGNFEAFGLAEPEIKVQVQAGEGQITAFELGNRTTDDSAVYARMAGGKEVFTVPLN
                     LETQLSKSFTDLRDKRVLPANLNEATTVTLQFEMTTLTAEKSDGKWRLREPIAQPADS
                     AKMSEILRNWNNARASDFIDSDTLNLADFGLEPPALRAIAYVADEATTAGRLLAMRIG
                     DTPTTGAAKRWAMAEGEGGIFQVPATLLTQLQPDLRDLRSKEIFSMTSAEVGKAIFNV
                     NGQVLALERDSADVWHLADDPDAKLDQGTVGAKVSSLTNLQVKEYLELPPDPAETGLD
                     SPNLIATLVTRDGATSQTVITGRKAGDADYVYARRGDDPEIFGVDWTRPGDFFLTRDD
                     VIDRSIFDFDQSLVQTLRIRDGERELTLKRTDSGAWNVFRGEEDDRIGQVEGSYVTPL
                     LYAVTGLDWDLQFDAGDSEVQQYDLITPSRELTMLDEEDVELGRLGQGESSDIYVHLV
                     RGGNAFFAVGMDDYASVSDSLQSLLDQIELK"
     gene            complement(30676..32124)
                     /locus_tag="PLS90_00620"
     CDS             complement(30676..32124)
                     /locus_tag="PLS90_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPK00935.1"
                     /translation="MIKQSVMWGKLGRVLRSGAPIIESIEVIAEESDDPVLRDAFFRI
                     ARGARDGQMISDTLSEMGEIFPRSLVVMIRAGENSGLLEEVLLQIPEAVREGRIGLSE
                     EEQPASEAELARQPEARAATESLLRRALARGAAAVHLTPAADAGAVHLRIDGVLVEES
                     RLEPEIYRAVLARLKKMADVDLMNAPPLIEGHFRLLLDREPVDLRLSGLRSSAGEAMV
                     VRLFRPERDLLRLDQILIEDADRRRFARWIERPQGLILVTGPGGSGRSTLLHALAHHL
                     LAAGGRKLVTVESPVQLRFDGAVQLEALPSGEPLPARIHAALRLEPDVLLIDAPEEPD
                     ALRMALRAAAAGRLVVALMHSPGSVEALRLLLDIGLPWWQLREDLIGIVSQRLVRRLC
                     PACRRPPAGAHGPSAECDVCRGAGYQGRRAVFEWLEPDAGVWDAARRPLEEAELRRAC
                     QAGGMVMLAEAARRLAAAGETDEAEINRAVRG"
     gene            complement(32223..33314)
                     /locus_tag="PLS90_00625"
     CDS             complement(32223..33314)
                     /locus_tag="PLS90_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HPK00936.1"
                     /translation="MGDRRYAVSLKRRAILKSGLALLAAGAVGRAAMENDALTSATAS
                     IPPEPALPTGWIITVEGPIPAAEAGIMLPHEHVMVDFIGADQVDSSRYDADEVFSAVR
                     PHLERVQALGARTICECTPAWIGRDPELLRRLAAATGLRLVTNTGYYGAGANKYLPAH
                     ALTESADQLAARWIAEWRDGIGQTGIRPGFIKIGVDAGPLSDVHRKLVAAAARAQRAT
                     GLTIAAHTGDGIAALEELDVLRAEAAAIERFIWVHAQSEPNPDRLLEAARAGAWVELD
                     GIGPDTLDQHLTLMLALREAGLLGRVLLSHDAGWYTVGEPGGGAYRGHDTLFTDFIPL
                     LRERGFSQPEIDQLIIHNPAKAFAVERLS"
     gene            complement(33685..34509)
                     /locus_tag="PLS90_00630"
     CDS             complement(33685..34509)
                     /locus_tag="PLS90_00630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPK00937.1"
                     /translation="MNNTLFEQSLAALRRGLPEPETADLAIVTGSGLSRLVEIGEDEE
                     SISFSELPAVGQGAVAGHGGRWHIVRLGGRRAHVLRMRRHFYEGVAQSQVIAGVRLLA
                     RLGVSRLILTNAAGGLSPRLRVGDLMRVTDHINGQHRNPLIGPNDERNGPRFPDMSEP
                     YDREMGELIDETAREQGIDLKRGVYVALAGPTYETRSEARLWRTLGADAVGMSTVPEV
                     IAARHAGMRVAAISLITNSHVRPQGPTTHEEVLEVGERAGRRLLRLLQALIPRLPP"
     gene            complement(34640..35455)
                     /locus_tag="PLS90_00635"
     CDS             complement(34640..35455)
                     /locus_tag="PLS90_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK00938.1"
                     /translation="MTGSIALKRTGNYITDTLRHLGDFFHFTAKTFLLCFRPIRYRLV
                     MAHANQLGMNALPLAFTTALFVGMVIVLQTGHELAMFNAKQYAAAGAAKGLTQVMIPV
                     FTALVIGARTAASIAAELGTMRVTEQIDAMEVLDVNPMRYLVVPRLLAVMIMLPVMTV
                     YADVVGLAGGWAMGVFALGVPANQYLQITLDFMTLGDVISGLIMTFFFAAAMAICGCY
                     YGYTAGGGAEGVGRATTRAVVFTMMWLILLVYFLTSWILYLRGTIFAPRLLVG"
     gene            35918..>36800
                     /locus_tag="PLS90_00640"
     CDS             35918..>36800
                     /locus_tag="PLS90_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00939.1"
                     /translation="MATQQDAGALTNPFRPVRASLADLLGDEYIEAVCAGRAWLAGED
                     LDALREEARRPVDFLPAAFIERQHELLGHTGQSVPAAVLTSSPAGRSTDRFNEATKTH
                     VAPLTGFGLFRLAESGRLHLITKSEHYHALLGHSFPGYRLIDRARALGIPNATHNNTR
                     GYITRLLEERLVAAANGTPGADADKLDLNRVLNLETGSLAVEAALKMILARFHRIQGD
                     ADPPAYEGRIPVVLVLGNENGGLEANYHGTTLLTQTMRGMWASLLDRLQAAGIFEVRA
                     IRPNRLEDVEAAFAECEG"
     assembly_gap    36801..36810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <36811..37519
                     /locus_tag="PLS90_00645"
     CDS             <36811..37519
                     /locus_tag="PLS90_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK00940.1"
                     /translation="GGARKIAAFFHEIVMMNYGAVLLEREFLRRTYELCRAHDVPTIV
                     DEIQSCMWSPEFFLFREYGLRPTMAAVGKGFPGGEYPASRILFNASMDGRMPQFAALV
                     TNGQEELAALAYLITMHWAAANADVTRAIGEYYEERLREFAERHADRVKGINGLRHMC
                     GLEFDSLAAAADFAASMVRMGFDISAQTYKVDCPPVVLTKLPLIVTRAAVDFILARME
                     QAITESAAPRTPRSAHA"
     gene            37543..38673
                     /gene="ald"
                     /locus_tag="PLS90_00650"
     CDS             37543..38673
                     /gene="ald"
                     /locus_tag="PLS90_00650"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPK00941.1"
                     /translation="MLIGIPREIKVKENRVACTPGGVRLLAGAGHRVLVERGAGEGSG
                     FSDEQYGQAGAEVVASAAEAWSAELVIKVKEPVPAEYQYLRPGLVLFTYLHLAAAPEL
                     TQALLDSGTTGIAYETVAVGGRLPLLEPMSEIAGRMATIVGAYYLSKAQGGKGVLLGG
                     VPGVLPGNVLVIGGGTAGINAGRMAMGLGAAVTILEVDLVKMRFLDITLQSSAHTLFS
                     NEQNLMDTLPTVDLLIGAVLRPGARAPRLIRREMLDVMQPGSVFVDIAVDQGGCAEST
                     RPTTHDDPVYVEHGITHYCVANMPGAYARTATQALTNATIPYAAKIANLGVERALRQM
                     ADLRQGLNTWGGGVIHPAVAEATGHPCQANPFDHPDGVPAAH"
     gene            38670..39443
                     /locus_tag="PLS90_00655"
     CDS             38670..39443
                     /locus_tag="PLS90_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPK00942.1"
                     /translation="MSPGIESHPRHRRILELLARDGEVSVQQLSALFAVTPMTIRRDL
                     EALEREGRLTRTHGGAVYSQRAVIEFAFLERGRERQAEKQAIAREAARLVEPGMRLVL
                     DTGTTSLAVAHRLTGTPRLTVLTTSLAIASALHAHENIELILLGGNVRRSSPDLTGPL
                     TEENLARFRVDIAFVGCDGADATGAYTTDMAVARVTRAMIGCARRVVLVADSSKFGRE
                     AFVRYAAWENFQQVITDGGLTSRARKWLKKARVELRCAS"
     gene            39482..41473
                     /locus_tag="PLS90_00660"
     CDS             39482..41473
                     /locus_tag="PLS90_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPK00943.1"
                     /translation="MWRARRARADAGRPPNDPALDQFMVSRHSKRKSLARLALYPLLA
                     APRLFGAGIGLDLEPLPEPAPPPAVTLPAETEPGAPEAAAPPAINLDELYEAGPDTPE
                     LGAAPAATPGPVRPPISLDLLEELALADAGWRLIRVGDAPTSVVIHGSWDAVRSGELT
                     PADFRPLSPPDLDAGLGLATVYGPRTAGWFVAGGRRLHVKSTDASIGFRNYSYESLAR
                     LGWAVGVARGVQPALRIVAAGQPVSAMNLAEFTRRAVARAPATDAWLIAGQVHLPRAV
                     GRALRLAPVGGHAMNGDDSPENPARSFFGPVSEPDIWALLVIVAVRPASGQPAAAALR
                     EKVFGARGEADDGDLLIRAHYGVFLPPAPAIDPDAPFQRIWDQSVRSPQFPRIASVRQ
                     LLPDSTIDAAHVSVWAVGQVLPYDLYQEPKLVEVTALDPTIQTEQMWATGATLVNEPI
                     YERPRLLLRREVAERLVRINARLKEQGYRLKLYDGYRPLSVTQRLWKLHPDPRYLASP
                     TVGSRHNRGAAVDITLTDLAGNELEMPSKYLTFDITSHRTWEGMTPTARANMNLLTQA
                     MRSEGFTTINEEWWHYDAPGWRDYPVLDVPLWPDEPARWVAEGNHDPAAAAEIAEKAS
                     RGDTSVSAPETGREDGGSEPADGSDGGEDARRRTPWAAN"
     gene            41571..42602
                     /locus_tag="PLS90_00665"
     CDS             41571..42602
                     /locus_tag="PLS90_00665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00944.1"
                     /translation="MSADTPQPEITVLIAAHNEAERIEGCLHAVLEQDYPMERVEIVL
                     VDDRSTDDTAARARRLGIERLRVLRIDEAPANLTARQAALDRGLIEARGEIVLITDAG
                     ARVPREWIRELTGHMGYRDAAVTAPVVFAGGGHFFTSIQTLDAVLTLTYYRWAARNHW
                     HTGVLGANLALRREAYLEVGGFPAIGFAPDENIALCQALRARGWSIRYLDSPTVCNPA
                     CTRLPELMLRWRRKTRNGPAGLTLVTLGLILSNLALLGAALVLGGGWWVIVLLRYALG
                     VVAIAGVVMQYGALNVAMAMVLFEPLMTLLGTWVYLSNLLVPRWRWAGVWYDRRGPLR
                     RGSLSHPAA"
     gene            42626..43798
                     /locus_tag="PLS90_00670"
     CDS             42626..43798
                     /locus_tag="PLS90_00670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK00945.1"
                     /translation="MRLLLIIYEYPPVGGGAGKSARGLARALTRMGHEATILTSRFEG
                     LPAEEVVAGVRVLRIPVLRRHLNYAAPWEVFSFAVAGMMRIGWLKWKVRPQVVLSYFT
                     IPGGPIAWLYRWRNGVPFVSLLRGQDVPGYPDTPRWLGWLMAPAARFIWRRSLFVTAN
                     SESLAELARRTMPGLEIPVVANAVDVERYQPAPGERGGPPLRILYLGRLREFKGIQDL
                     IAGFARLRESFSGPSELTIAGFGPHRVNLELLTSRLGLKDCVSFTGRLEEPEAVAHLQ
                     RAEIVVNPSYGEGMPNAVLEAMACGAAVILSDIPAHRELAREGEEALFCAPRDAASLA
                     AALQRLAEDPDLRRRLGERARERMVRDFSYEKRAEQLLALIAARTGGEPLPDERGG"
     gene            43872..44423
                     /gene="rfbC"
                     /locus_tag="PLS90_00675"
     CDS             43872..44423
                     /gene="rfbC"
                     /locus_tag="PLS90_00675"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HPK00946.1"
                     /translation="MQLERTPIDGLLILRPKVFQDHRGFFLESYNEGRFRELGIELPW
                     VQDNHARSVKNTVRGLHFQRGAGQDKLLRCVRGRVWDVAVDLRPGSRTFGEWFGMELS
                     EDNFAMLLIPAGFAHGYAVLSDVGEVIYKCSRVYDPALEDGVRWDDPRIGVQWPVAEP
                     ILSERDRTAQTLDEYLARHASGA"
     gene            44430..45314
                     /locus_tag="PLS90_00680"
     CDS             44430..45314
                     /locus_tag="PLS90_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK00947.1"
                     /translation="MIPAAAISARQPSVLSYLNPWRLARDLLAHWELIRQFTAREVLG
                     RYKGSYLGLFWSVATPLLMLGVYTFVFTVILTGGKWGNLAPRDASPAAEAHPADFLLN
                     MLCGLILFNIFAETISRAPTLVLSNINYVKRVVFPLHILPISVLLGNLIYGLTSLVLL
                     LAALLYFTHGVPWTIVLFPLILIALLMLCTGLAWFLASLGVFFRDIQHAVIVVVQLLF
                     FMTPIFYPAEMIPERYRWVLMLNPLAMLVTDARRVMIEGVLPVWSRYGIVLAISFVVM
                     LAGYAWFMRTRRWFADVI"
     gene            45339..46487
                     /gene="wecB"
                     /locus_tag="PLS90_00685"
     CDS             45339..46487
                     /gene="wecB"
                     /locus_tag="PLS90_00685"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPK00948.1"
                     /translation="MSVKRIAIVCGTRPEAIKLAPVYLELARRRDRFEPLLWATAQHR
                     QMLDQVLETFGLRADRDFNLMRPGQTLTMITTAVLEALEPALAADQPDLLMVQGDTTT
                     AFAGALAAFYARTAVAHVEAGLRTETKFAPWPEEMNRRLASRLTDLHFPPTERARANL
                     LAENVPADRIWVTGNTVIDALAIVVEQVRARRPEFPPDFPLDRLANGRRMVLITGHRR
                     ENFGPGFESLCRAIAELARRNRDIEFIYPVHLNPNVREPVGRILKGIDNVHLTEPLQY
                     QPFVWAMDRCHFLLSDSGGVQEEAPHLGKPVLVMRETTERPEALEAGTSRLVGTDFET
                     ILREATRLLEDEAAYLAMSRASNPFGDGRAAVRIAEVLERHLGVLAPR"
     gene            46484..47374
                     /gene="rfbD"
                     /locus_tag="PLS90_00690"
     CDS             46484..47374
                     /gene="rfbD"
                     /locus_tag="PLS90_00690"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPK00949.1"
                     /translation="MTGKRAEILVIGHRGMLGQDLMARLGARAAGADREECDVTRRDA
                     VEALLDELKPATIINCAAYTAVDQAESEPELARSLNADAVENLGLAARARRIHLITIS
                     TDYVFSGEGEAPWPEDAPASAFAPRSIYGQTKLEGEQRLRRIGGEFCVVRTQWLYGAG
                     GRNFVDTMAALSAEKPVLRVVNDQLGAPTWTRDLADALTLLAERRATGFFHVANSGFA
                     SWYDVTRHIVQRLGRACRVEPCTSEEFPRPARRPHNSRLAQGKFAALAGSAPRPWPAA
                     LDAYLSERHGRNTDGNPTFS"
     gene            47890..48228
                     /locus_tag="PLS90_00695"
     CDS             47890..48228
                     /locus_tag="PLS90_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPK00950.1"
                     /translation="MSKVECIIKPFKLEEVKEALTHFGISGLTVYEVRGFGRQRGHKE
                     IYRGTEYKVDFIPKLKIELLVTADQVDDVVDVVVNAAKTGAIGDGKVYVTPIESLVRI
                     RTGEKDIDAL"
     gene            48284..49618
                     /gene="amt"
                     /locus_tag="PLS90_00700"
     CDS             48284..49618
                     /gene="amt"
                     /locus_tag="PLS90_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013044300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPK00951.1"
                     /translation="MKSNGMKGLTGRLFRSRALLGAAALLLMAGAAWAQEEAGPSAEQ
                     FSLNNLLLLISGALVFWMHAGFACVESGLTQAKNTVNILSKNVIIVCLGGVSYFIIGF
                     GLMYPGEFNHWIGFGGSGFGPVSPEDYGDYTIFTDFFFQAVFAATAATIISGAVAERI
                     KFASFIVVALLFVTFIYPIAGSWVWGGGWLADLGFKDFAGSTVVHSIGGWAALTGAVI
                     LGPRRGKYTADGIRPIVGHSMPLATIGVFILFLGWFGFNGGSVLAMDSGAVSMVYVTT
                     FLAGCAGGIGAMLASWIVLRKPDITMILNGILAGLVGITAGADTIAPWPSLAVGAIAG
                     VIVVFSVVTLDRIKVDDPVGAISVHLVCGIWGTLAVGIFSFNPDHGFLTQLIGIVAIG
                     VFVLVTSTLAWLALKHSPLGLRVSEEEEVNGLDTYEHGLEAYPEFKSTTGIL"
     gene            complement(49931..51847)
                     /locus_tag="PLS90_00705"
     CDS             complement(49931..51847)
                     /locus_tag="PLS90_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00952.1"
                     /translation="MSPLSKILSWLPRRRDGGRLRLPSTAPLMRQLRRVDWRRLGGQF
                     LRYLPGAMILLALIFALMTLDFPAAMWERWLPLPLTVVGVALMIWRVGAPDFDLRNLA
                     WLLLILLPALVRAILSIRGPLPGMGIGEALELAVMATVAVGLWFLARLRPWQAWWFWL
                     GALAIAWLVGRSMHQRLPTASPWVVATRWSVVLGLGAAAVISALRAISRRPAGHGIDA
                     VHSTPDPLRIITRPIRIGLHLAMVLWGGSVGWNHFISQQRLPVDRALMLEAGIRAQEA
                     LWMQSVLFGWGHGTAGRLGEVLAVPNPVMIPDWGGLFGLLAIGGLIGLTLLLVWIAFL
                     ILHREPRVARPPGPTGRMAEVAAPVALFVAGLQLTGGARSAVVLLVIAGWSALALADV
                     PTRMRTHSAAGLNRRLALALVGFIGVILSLLLFKPTHGQVILRQTAGADLPSAVMRGR
                     LLAAQRANPFIPEIPRALAVSYRKTMEESPGWSETNYLNVVRSYRRAIALDPYETQYP
                     LELARFQLLCRREEEALETVQAALARMPNSIELIDWLYNSALAQNRTSLAYNMQVDGL
                     ALAPGERRWWVRRYELTHAQGQIPLAAQALAVALTANPEEPALVTALWNSRHARPENP
                     AIPVTLPDGIEEEG"
     gene            complement(51993..53312)
                     /gene="lysA"
                     /locus_tag="PLS90_00710"
     CDS             complement(51993..53312)
                     /gene="lysA"
                     /locus_tag="PLS90_00710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPK00953.1"
                     /translation="MTAPERDPRSQALKLDGVPVATLARKFGTPLYVYSHSTLVANYD
                     RIRQAFAEIDPLIAFSTKSNTNGTILRILLERGAGLDIVSGGELERALRAGADPARII
                     YAGVGKTREEIAAALNVRILAFNVESPAEAEAINEVASGFGRKAPVAVRVNPDVAADT
                     HHYISTGRKETKFGIPYHDVRRLCRRLARMPHLRLIGVHAHIGSQITQTEPFVKALER
                     VVQLTHQLRRDGLSIDLVNLGGGFGIGYEESTQPIDLEELARRMMPMLRELRAQVIFE
                     PGRSIVGPAGFLLTRVLYIKEAQTKCFAIVDGAMNDLLRPSLYSAYHRILLDGRPGRG
                     RRRVYDVVGPICETGDFLGKDRPFPPLKPDDLLLVCDAGAYGMTMASNYNSRPRPAEV
                     LVRQDKAYLIRHRETIDQIIGCEEIPPFLNGSGAEQRSARSPRKVRA"
     gene            complement(53447..54334)
                     /gene="dapA"
                     /locus_tag="PLS90_00715"
     CDS             complement(53447..54334)
                     /gene="dapA"
                     /locus_tag="PLS90_00715"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPK00954.1"
                     /translation="MQPSASLQLQGSIVAIATPFRDGKVDEAAFRRLVEFQIENGTSG
                     IVPCGTTGESATLSHEEHEQLVRLTVEIVAGRVPVIAGTGSNSTREALRLTESARADG
                     ADAALLITPYYNKPTQEGLYRHYRKIAETVDLPLIVYNCPGRTGVSIAPETVARLAPL
                     KNIVAVKDATASLDWTSEVCGACDLTVLSGDDSATLAQIAVGARGVISVLANVAPGPM
                     AELVAHALADRWDQARTLHHRYFRLMKTLFIETSPIPVKAALEMMGLIGPELRAPLCE
                     ISEASRAKLREAMRQVGLL"
     gene            complement(54563..54985)
                     /locus_tag="PLS90_00720"
     CDS             complement(54563..54985)
                     /locus_tag="PLS90_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00955.1"
                     /translation="MARRDVPPPYPRDKEELIRDFTVAGRLRDIRDSVRLRDQLMPRW
                     WEAGVGVLQFGAALGLLASLVQYAGLREDPMGHFIVFWFILTLLSLVLGFEFLILRLH
                     QMRRAHVVALRLIEKLDERIAALEGDAREQEGAQETSR"
     gene            55253..56113
                     /locus_tag="PLS90_00725"
     CDS             55253..56113
                     /locus_tag="PLS90_00725"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK00956.1"
                     /translation="MASQPVKPAHSNPLLAAARLLALVGLTFFLYPFYYLHYRLTPRD
                     RRYPISARWVHAWAVMACAVFGIRVRRHGPPPPPGALLAANHVSYADILALSSAIPCL
                     FTPKSEIKSWPLAGFFVRTTGHPFVTRARGRDIIKTTRSIERRLRAGTHVVVFLEGTT
                     TGGDRLLPFKPALLGAAESIQAPVVPVALRWSSRHPRVAVQRDIAYWGRHVIGPHVWR
                     LVGLRGTQVDITFHEPMRLDAGERRRLLADRLRRRIAATLDLPLALPETNPPQPASEG
                     KETDERAGET"
BASE COUNT         9548 a        18042 c        19227 g         9339 t
ORIGIN      
        1 tgtcgcggca ggagatgatc gccgcgccgg tggacatcac atcgggcttc atcagaccgt
       61 cgacgcgcgg ccccaggctg ctgaactcag accgcgtgcc ctcgttcaac gagttgtaaa
      121 accagccgtc gcccttgtaa tccgtccacg ctttgcggct cgtccacgac ccgacggcga
      181 tggcgtcgtc ggccagcccg ggactgccga tcgtatagga ctcatcgggg ctgtcgaacg
      241 tgaccaggcc gctggaggtc agtgcgaaca gatgaaccag cggcgtgctg ccggaacccg
      301 cgctgttggt caactcaaaa gtataggact tcgtcgcgcc ggcggcgatc tcggggaaca
      361 gaccgtaatt gcgactttcg gtcccgcgca cactgctgtc ggaggtgccg aggagaaaat
      421 cttcggttcc cgtcagattc gtgcaattca ggctgatgtt gaaatcgccg ggggtgtcat
      481 cgatccagac cacccggatc cccagagccg tggtgtaggt gccgacgccg gccgaattgt
      541 tgatcgtcag gccgaacgtc gacgacgtcg cgccgggcgc gacgctgacc gacgcatgat
      601 gcttcttgtt ggcttcattg ccggccgaca tgaagacaac catcggcttg acctggccag
      661 ggttgaggcc ggcgacggcg gcgtcgattg cctgctccat ctcctcggag ccgtcgttga
      721 acgagccaac accgccgtaa ctcatggtga aaatgtcgca gcccacctcg cccgcgcgaa
      781 cgatcgcctc gacttcatcg gcaatgctgg cgctggcggt aaagtcgttg ccgattttgt
      841 aaaaatagag attggcgccg ggcgcggcgc ccttgtactg cccgccggac tgcgtgccgc
      901 gtcccagggc cgaaccggtc acgtgggtgc cgtggtcgct cacggtgctg ctgacgtcgg
      961 tgccccaggt gccgacgtcg gtgccgtcgg tcatgtcgta cgcttccacc ggtgcgggaa
     1021 tatcggggtg agtcgtgtcc aggctgctgt cggcgacggc gatgttgacg cccgtgccgt
     1081 cgcgcgccgt gacgccgatg ccgttatgca cgtcgtccac gttcatcaac gccaggccga
     1141 ggtcgttcat cggttgatcc agccgcgccg tcgaggtcag cagaacgatc cgctcgtcgg
     1201 cctgcacctc gtccaaggcg tcgtaggcga ccgtcgacag ataaaagcca taggggtgct
     1261 tatccggcac ggggggaacg taggtctggt gaaccgtgat gccccgcgcg gcgagttccg
     1321 ccttctcctc ctgggtcagc gggcgctcga cgtagagcgc gcactcctcg gcgccgagcg
     1381 ggccgggcgc aatgccttgc tcgagcagcg ccgcttcctg ggaactgggc gaaacgcctt
     1441 gctcctgcaa cgactccttg atctgcttgt gatacaggat ctgcccgctg attttggaag
     1501 cgggcccctg agccgcggcc gtcccgccga tggcggccag caccagggcg gacagcagaa
     1561 tcaggcggcg ggattgcgcg cgcacggaca tggaagcatc ctcctttgga tggaagggga
     1621 gaccgttgca ggatgttgca ggcgggccag ttctgccgcc tgacgcttac tccccggcga
     1681 ccgagccaag cgaggagaag gacggaccaa attaatagcg gacaaacttt acatcaaggt
     1741 gaacattata tgcccgaccc gcgggagagg caaccacccg ggtgcttttt tctttttttt
     1801 cccgatggcg gttcccctag aatgccccat gcgctccttc ccgccgtcgt ttcgcgctgg
     1861 cagcccggcc gaggcgcgcc atccggccgc gcggcctcat atgaaagtgc cgtcatgatt
     1921 ctattggagt tgcatcgctg gggcggatcg ttacggcgga ttttgctgac cgcgtggagc
     1981 cttgcgctgc tggccgcttg cgggccgccc ccgcccgccc tgagcgagaa cgaaccgatc
     2041 gccattgcgg agtatgagcc gcagcagacg ctggccggcg agacgttcgc cgaggtcccc
     2101 ggcggcctgt cgacggttac gatccgcgcg cagggggcga cctccgacac catcgtccgg
     2161 ctcaacggga cggcgctggc cagccgggtc gacgcctacg gcattatcaa ggcgttcatc
     2221 ccggcggagc acctccagca gccgggccgg ctggcgctga gcctctacga tgaaccgacc
     2281 agccgcgtca gcgagccggt ctatctggag gtcgagggga ttgtcgtgga acgttacggc
     2341 ccgacggaga tcaccgcggg ggtcgatttc aacccgcagc cgtcgggcaa ctcgggcatc
     2401 tggctgcagg cgcgcaacgc cgcgcgcggc acggtgctgc tggccaatgg cgagccgctg
     2461 aagacggccg tgaccgacag cggcctggtg acggcggaat tgccgccccg ctattatgat
     2521 gccccgggca cgctgacgct gacgctggtc gaccgcgacg ccgatctgcg cagcgccgac
     2581 ctgcacattc ccatccttgg ggtgcgcgtg gtcgactacg gcccgcggcg gacggacccc
     2641 ggcgtcgcgt tcaatccgca gcccgacggc gcctcggccc tgtggttcaa tctggccgcg
     2701 ccgctgtgga cggcggtcgt cagctgggac ggtcgcgaac tgcagacgtc ggttggggcc
     2761 gacggggtcg tctcggcgct cgtgccggcc gagctgatcg cccgaccggg cttccatcgg
     2821 ctccagatcg tcgactcggc ggggggaatg aagagcgagc cgatggtgtt tcaggtgggc
     2881 gacgtcgcgc cggccgccgc gacccccgtg ccgctggcgc ccgcgctcga ttccatccag
     2941 atctatgtga agcgcgacgg catggtccgg ctggcgggtt ttgatctggc cagccgcggc
     3001 ctcgacctgc aggccacccg cggcgaccag ctcaacctgc atctgcgcgg ggagcccgtt
     3061 cccattgtgg ttgaggggct gagcccgagc gggacgctgg cgcccgacag cacgatcctc
     3121 ttctggggcg agtttccccg cggcgagcag accctgcatg acctccacgc cgccgaaaat
     3181 gtctacctac tgcgctggga tggcggccgg ccgccggtgc gctacacgat gagcgacccg
     3241 ctgagcttgc ccgcgccgga cgagccgatc ctgaccagct gcccccgcac cgcgcatctc
     3301 gaagaagatc gcgaggccaa cttcctgcaa cactacaagg gcttcccgac cgaccgggtg
     3361 atgtgggcaa ccttcatggc gcccccgcgc gaagggcacc ggttgacgct gggcacgctg
     3421 ccggacctgg ccgagggggc cgcccccagc gccatccgca tcatgctgtg gggcaacagc
     3481 cggctgggga tccaccccga tcaccgctgg cgactggcgc tcaacggcca cgacctgggc
     3541 gaagcggtgt gggacgatcg cacgcacgtg gtccacggcg ctccgattcc cgagcgagca
     3601 tttagccggc acgaaccgat gatggcagcc ggttccgaag tcgagctgac gcgggacaac
     3661 aacctcgaac tgcgaaacat gcaggcgggc gagacgatcg actcgatctc gatcgattgg
     3721 gttgagatcg attacgaggc ggtcctccgc ccgatcgacg accggttgac ctatcagttc
     3781 accactggga cgctggcgca ggccgtctcg atcgagaacg cgttcagcgg cgctccacgg
     3841 atcttcagca tcgatgaggg gatcgagctg gcgctgcccg ccggatcgcc ccccgatgcc
     3901 attcgttatc gctatgaagt gaaaccggat tccgccgtga cccgcccgag gttcgtcgcg
     3961 gtcgggccgc ggggctacct ggcgccggag cggatcgagt cgggcttcgc ctccgatctg
     4021 aagtcggccc agccgcccga gtatctggtc atcacgcacg cgcgcttcgt ggaagcgctt
     4081 cagccgctca tcgagcaccg caggcgccag gggttgagca cgctgctggt ggatgtgcag
     4141 gacatctacg atgaatacag cgacagtctg ttttcgcccg aagcgatccg ccggttcctg
     4201 gacgacctgt tcgtaaagga aggccagggg gcgggcgacc tgcaatacgt cttcctggtc
     4261 ggcgacgcga actacgacta tcacaacgtg cagaagaccg ttcccaatct ggtgccgacc
     4321 tatcacatcg agagcgagcg gtcggtcggc gtgccgatct tcgccctgga cgagtactac
     4381 gtctacgggc cggaggggac gacgccgcgg gtggcgatcg gccggctgcc ggccagccgc
     4441 cccgcccagg tgaccgacta tgtcgagaag ctggtggcgt ttgaggacgc caatcgggaa
     4501 gaagcggtcg ccgcgatgga gggtccccgc cgcgccctgc tgatcgccgg gaccgggttc
     4561 gcgaactact gcgatcaggt ggccgaaaaa ctgggcgagc cgtgggagat cacgcgcgtc
     4621 tacgccgagc gggacgtcgc gcgcaatgag gcgctgcgcg ggcaaatcgt cgccgcgctc
     4681 aatggcggct gcgacctggt ttacttcgcc ggccacggca gctactggga gtggcggacc
     4741 ggggccgagg atatggcgtc gcagacggac atgctgacca acgcgcatgt gcgcgagctg
     4801 accaacgccg gccgctatcc gctgattttc tccgccacct gctattcgtc gctgttcgac
     4861 tcgccggggt atgcgcaccc gacaaacaac gagtgggtcg attcagggat cggcacgtac
     4921 ctggtggagg cgcccggccg cggcggcatc gcctgcatcg gccacgtggg cgaatcgctc
     4981 gtgctggcct cgcacaagtt catcctgcgg atgatcgagt cgatgcggga gcagaacgta
     5041 gtccggctgg gcgatgcctt tctggccgcc aagcgcgagt cggtgggggt gccgtacctg
     5101 gggatcacgc tgatcggcga ccccgccacc tggctcggcg tccgctttag ccagggagtc
     5161 gggccggcgg aacccgcgcc ggccgagccg gctggagcct gatttttttc acgaaagctg
     5221 agagcgcgct cagctcgatt gagtcggcgc cggggccggc gcttcggcca cgccgaggcc
     5281 ggccggttcc gccgggctgg ggatgatccc cttctggata tagatcgggc ggatcagccg
     5341 gcgcagatcg ggcagacggg tggcgaagcc gagcgcgccc aggatgcagc atgccccgcc
     5401 gcagatgacc atcgcgcggg cgccgaccag ttgcgccacc gcccccgcca gcaggctccc
     5461 cagcggcacc atgcccatga acgacatcga atagaagctc atcacgcggc cacgcttgtc
     5521 gtcgtcgacg atcgtctgca gcacggtgtt ggtcgaggcc atctggagca tcatccccag
     5581 gccgatgacc ggcatcgcgg ccagcgtgag ccagaaccag ctcgacaggc cgaggacgat
     5641 caggccgccg cccatcacgg cgggcgacca ggcgatgacg cggcccaggc cgcgcacgct
     5701 ggggcggctg gccaggtgca gcgcgccgat gagcgcgccg accgccgccc cggagatcag
     5761 aaacccctgc gtgcgcgaat cgccgcccag aatccgctcg gcgaagatcg gcagcagttg
     5821 gccgtaggaa ctgccgacca ggctgaccag cgccagcagc aacagcaggg cgcggatcgg
     5881 cgcgaagccg aaggcgtaac gaaacccctc ggccaggtgg gccagcagcg ccgggccgcc
     5941 cgcaacccgc tgacgaccgg gcacccgcat cagcagcagg ctggcgatga cggcaaaata
     6001 gctcaggcca ttgatcagga agcagaccgc ctcgccccac agcgcgatga gcacgccggc
     6061 cacggagggc ccgacgagcc gggcgccgtt gaccatcgat gagttgagcc cgatggcgtt
     6121 gtgcaggtcg tgcggatcat cgatcatatg gatcaggaac gcctggcggg tcgggatgtc
     6181 gaccgcctgg atgacgccca gcaccagcga caggacgatg acgtgccaaa cctggatgtg
     6241 gccgctgaac atcaacccgg ccagggcgag ggcctggacc agcgacaggg cctgtgtcgc
     6301 gatgatcagc cgctgctggt tgaaccggtc ggccaggacg ccgccgatgg gggcgagcag
     6361 gaaggtgggg atgcgggcgg cgaagccgac aacgcccagg agcatggcgc tgtgatcgag
     6421 ccgccagacc agccaggcga ccgccgtcat ctgcatccag gtgccgatga gcgaaagccc
     6481 atggccgatc gcaaagagcc ggaaattgcg gtggcggagc gaccggaaaa gatgcaggaa
     6541 tcggacgggc aggatcatgg gatcaggggc atggggagat ggcgctcgcg ccgggggtgc
     6601 ggaccggccg gcccggcgcg atggggatta tcccacaacg agcgcccgcg aggaaatgca
     6661 ggaagcccgc ggcggcgcgc ctcgacgcgc cgagggctga tcgcggcggc ttttccttta
     6721 ctgatcgccc cctgggcctt catgatggat gggtgttaaa ggttgcccga gcgaggggaa
     6781 tcgccatgtc cgttcatgca gttggatccg cggtgcgcca gtccacggga ctggccgtga
     6841 tggtcggcct ggtgatcttc agctacttgg ccgtcgtttc gatcatcccg gtccatcgga
     6901 gccggcagtt caaccagcgc gaacgcatca cgcagcggct gatgcgaatc gaaatggcca
     6961 agcaggaggt cgcgatgcag cgcgacctgc ggccgggagc gcgcctgacg atggaggagc
     7021 tggtcgcggg aagccagtac ctggaccagg cgccggccag cccgtcgggg acctatgagc
     7081 cgggggcgct gggcgagctg ccccggttcg tgccgcacga gggcgaaccg atcgtccccg
     7141 agccggtcag cccgcgccag ccggcgccgg cccagcccgg cgacgcgggc gaggacgact
     7201 cggccgcgcc cggcgaatcc gcggacggga acgcctgatg gcctcggcgc cgacggttgc
     7261 ccccgccctc aagcccgccg ggctgtcgcg cgctcccgcg cggcggagcg gtgaagccgt
     7321 cgtcggcccg cggatcgggt tcgacgcggc ctcagtggtc gggccgcgca cgggcatcgg
     7381 ccatgccacg gccgagctgc tgacggcgct ggccgagatc tggccggaaa actggccggt
     7441 ggcgcgcgtg tgggtcaact cgcgccgcca cgcgctcccc gagcacgacc cctggatgtc
     7501 ggccagggtt tatgacgtca agcggacgac gctcccgggg cggctgatcc tgcgcggctg
     7561 gcagtatctg ggctggcccc cggccgagat ctggaccggc ccgatcgacc tgctgcactc
     7621 gccggcgagt tacatcccgg cggtgcgcgc ggcgcgccgc gttgtgaccg tccacgatat
     7681 ctacttcaaa cacgcgccga cgctggtcga cacctacggc gggaaatatt tctaccagac
     7741 gttcgaacgc gggctgcccg aagtcgacca gatcatcgcc gtctcggagt tcacgcgcgc
     7801 cgagctgctc aagcattacc gcgtttcgcc ggagaaggtg agcgtggtgc cgcacgcggt
     7861 gaacctgaac cggttccgcg aagagccggc cccggacgac aacgagcacc tgcgccaact
     7921 ggggatcgaa aagccgtacc tgctgtgcgt ggcgacgatc gagccgcgca agaacctggt
     7981 gacgctgctc gaggcctacg cccgcgcgcg gcggatcatg gaagcgggcg gccagaagct
     8041 gccgcggctg gtgatcacgg gccagccggc gtgggggatc aaaccgtttg aggcgcggct
     8101 gcgcgaagcg aagctgcagg atctggtgac gccgacgggc tacgtgcccg acgcggcgct
     8161 gccggcgctc taccggcagg cgctggggtt cgtctttccg tcgctctacg agggattcgg
     8221 gctgccggtg ctggaggcga tggcgtgcgg ctgcccgacg ctgatctcgc gggcggggtc
     8281 gctgccggag atcgccggcg acgccgcgca ctacttcaac ccgcgggaat cggggtcgat
     8341 cgcacgcgag ctggtgacgt tcctggccga tccgatgcac cgctcggcgc tgcgcgacgc
     8401 cggcctgcgc cgcgcccgcc agttcacctg ggccgccacc gcgcgcggga cgctcaacgt
     8461 ctaccgccgc gtgctggggc tgaccgaatg cgcgtgagcg aagttgaatc cctcgccggc
     8521 ctctcccacg cccctcctcc gcaacaatcg catgctggtt gtgacggacg gcgtgtggga
     8581 gggacgtcaa ggatttcgac accggatgcc gatggcctgg gcggaagcag gcaatcacat
     8641 tctcctgctg gaaggttcgc cgtatccgaa ccatttcatg cgcggcgatg cccgctgggg
     8701 cccgtcgttg cacgggcagc ttcgccgggt ccacccccgc ctgcacgtcg gcgcgatgcc
     8761 gcccgccttt ccccggatgc tgaagggggg acttatgggg aatgccctgc gggcgctgca
     8821 tcggccgctg gatcagcggc gcatccggcg atacctgcgc gtgctgggct gggagccgga
     8881 gatcgtggtg ttgatgcagg agccggtgcg ctacgatctg ctggagctct tccccgacgc
     8941 gctgcgggtt tattattgca acgatcttta cggctacggt cacggcactg cgaatgaatt
     9001 ccgcgaagag gcggaatgtt gccggcgggt ggatgtggtt ttcaccacct ccgatatcct
     9061 gcggcggcgg cttctgccgc acaatgccaa gacgttccac attccgcatg ccgttgacct
     9121 ggcctggtgg gagagcaacc ggaaccgcca tcccgccgag atggaacgaa tccgccgtcc
     9181 ccgcgcgctc tttaccggcg cgttgacggc tcgaattgat ttcgcgctgc tggaggaagt
     9241 cgccggggcc tgcccccatt ggcaattcgt cctgattggt ccgtacaatc tagccgagaa
     9301 ggaggcgcgg cgcctggggg cgttagggaa ttgtcatctg tttggggcaa agccccacga
     9361 ggagatcccg ggatacctgg ccctcgccga cgtgctgctg tttccctatg ccggcaaggc
     9421 cacggcgcag cattgcggtt tgccattgaa attttacgag tatgccatca gcggccagcc
     9481 gatcctgagc accgatttcg cgcaactcga attgccgaat ccggatctgg ttcacgtggc
     9541 ccggtcgccg caacagtggg tggattttct gactcatttc gagcggcacc gggagcagca
     9601 agccgttctg acacgccagc gcagggagct cgccgagcgc aacacctacc aggcgcgaat
     9661 cgagcagcag cggggcattc tcagccatct tgatcgggcc tcgcgatgag acgcgtccga
     9721 aaaccgctcg tctctgttat cactcccgcg tataacgccg cgcaattcat cgcggagacc
     9781 attgcgtcgg tgcggacgca aagccacgac cattgggaaa tgctgatcac cgatgatgga
     9841 tcgagcgatg cgaccgtcga gatcgtggag cggatcgccg tggaagaccc gcgcgtcaag
     9901 ctgctgcgcc tcgctcggca cacggggctg gccgccaaag ctcgcaatca ttccatgcag
     9961 atggcctcgg gcgactatct tgcgttcctg gatgccgacg acgtctggga acccaggaaa
    10021 ctggagcaac aggccgccta ccttgaaagc caccccgagg tcgatgcggt ttgctcatgg
    10081 tattacgtat tcggcgatcc cgcccgcgtg caacgctacc gcgaaatgat gtggcggttt
    10141 gagacgacgg aagtgacggt cgaccagatt ctgatgcagt cgcttttcac cagcacggtg
    10201 atgatgcggg cgcggtgctg tgaagagctg ggcgggatgg acgagtcgcc ggcgctcagt
    10261 tcagggcagg attatgagta ttttgtccgg ctcgtcgcgc ggtaccgcgt gcaccggctt
    10321 tgcgaggaat tgagcggcta ccgactcgcg ccggcgggcg gatccttgtc gacgaagggc
    10381 cagcggctcg agcgccggcg gcagcgcgag gaggcgattc tggaagcact ggccggcaag
    10441 caagtgctga cccccgcgct gctgcgtcgg cggcgggcca ttatgcacta taatctggcg
    10501 cgggactgcc tgttcgaact ggatcagccg tatcgcggct ttctctggcg ggccgttcgc
    10561 acatgggcgg ccccgcccaa agcttatgtc atgtttctat tatcttttct gccggcgcgc
    10621 caactgcgct gcgtattgac taagttgctt gcgcttcggc aatcgcgctt gttcaccggc
    10681 cggaagtgag attgaataag cacgaactac agccggtggg aagagtcatc accggctgcg
    10741 ttccatgtgc tggttccttc gatgccacaa cgccgaaaag tcgtggaata ttgagggata
    10801 tccgtcggcg gaaggagtaa atgcaataag cagcgacagg cgccgctgat cccgggccat
    10861 ttgaataccc ggctgtatag ttcgagagcg gcaatctccc actgcggcgc gtggctgacc
    10921 tgcttgaatt gccgccaaag cagatgtgcg tccgtcggac ggtctaatgc aataaactcc
    10981 acggcggtga agaagaacga gaaggccaac gcgcgccggg tcttccgatc cattgaatga
    11041 ttcttatcta tcctttgggc ggtttcgatc aacaatgatt cgacttgctt gagatgctcc
    11101 acggagcggg ccgccgcgcc gatactgccg gctgccgaag gaagcctcca ataagctcgt
    11161 ccgccgggga atcgctcgta ggacaggccc gccagcagag cacgcgtatg aaactcccaa
    11221 tcctgccaac ttggcaaatc ctcattccat gggcccagcc ggtcgagagt cttccggcgc
    11281 caggtgggac cggcggtctg ccatggaatg tcgcgcttga gaaatcggat caggtccgtt
    11341 tcggcagtaa caatattgtg aaggcgggca agatcacccg gaacgctttc gaagatttca
    11401 acgggagaaa cgcagaaatc aaggttgccg cgctggccga gaaactcaac gcgatcggcc
    11461 agacagtctg gcgtggaaat acaatcatcc gaatcaataa acataatgat ctcaccgcga
    11521 gcatggcgga aaccaaggtt ccggcaggcg ccggcgcccg atttccccgc ctcacgccta
    11581 attaacctga ttcgcgcatc acgggaacat aattgtgaga tcttcaataa ctcatcggcc
    11641 tcgcttccat cgtcaacgac gagtgcctcc caggaaagaa gggactgatg gacaaggcta
    11701 tcgacaaggg ccggcattag atgcgcacgg tttttaacgg ggatgatgat cgataccctg
    11761 ggattatcca tgtcagctag tcaaccttcg atcgttgccc gctaaggcgc gcccagaact
    11821 ccccccaata ggtcaggtgg ggcaatattc tacggtaaac gtatccctgc cagcatggat
    11881 cgaccgtccg atcgcggaac agcatgccgg ccaggtggat cggcacgaac accatggaat
    11941 agagtaacgt ccgcaaacga gcgcgactgc ggcccagatt cgtggcacgg gcctgaaggc
    12001 cggactgcca catgcgccgc gcgcgatagg agagactcat tttctccggc gccatgaaat
    12061 gcagaatctc catgcgcgga aaaaagtgcc cgttattgcc tcgttccttg agggccagtt
    12121 gaagatacgt ttcctcgccc acgccgatgc gcgtgtcgat catgcccagg tgctccggaa
    12181 agccgcccag ggaccgaatc agatcaaccc gccaacccat gttgccgccc gacagaaaac
    12241 cattgggcgg caacggtccc tcctgctttt cataatgcat gctgccatag gaatcagcaa
    12301 accagcgtgg cttgccaccc agataatagg gccggtaggg cccgccaaaa aaggggggat
    12361 gccatcgggc gataccctcc tcgatcgcat ccatccaaaa cggcaaaggt ttggcgtcat
    12421 cgtcgaggta gacgagatat tccgtgcggc aaagggcaag cgcggtgttt cgggcgcgac
    12481 tgagacctac tttgggctcc aacacatatt gaatattcag ggacccacat ccctccacta
    12541 tcgcacgtgt ctcatccgtt gatgcattgt cgacaacaat gacaggtgtc gggcgtgaag
    12601 ccagagcaat cgtcaaggcg tccaggcatg gcttcaacag ggccgcgcgg tttcttgtgc
    12661 agatcgcaat ggatattgtg tcagttgtct tcattcagaa ctcgacgttg catacggatt
    12721 tttctgaatc ctcggcccca tttccttcgc atccagttgt atccgtttcg cggcgtgtag
    12781 tcgcccatct gccagcacag cgagcgccac aggtcgggcc agcgcaggga aagaccaggg
    12841 tattccttca ccttttccag gatcacctcg gcccggtcgc gatatccgcc ggaagcgagg
    12901 agttcagcag ccaagagata atgctgcaag tagcgatagc tgttggtgtc gctggggaag
    12961 tcttgaatcg aggacagcag cgcgaggtct tctcgattgg ctccatattt cgacagttga
    13021 cggagccaga tcgccagcac ggcctgggaa agcaatccga tattcacatt gttcaaaatt
    13081 gtctttatat cgtgatgtgt cgcgcattcg cgtaacagcg ctttaacgga ttgtagcaaa
    13141 tctgaccggc tgtcctggga caggtgtggc acaaggctct ccgtcacatc ttggtattgc
    13201 agactcgccg cccacagaag gatgcccaca agcatgtcgg gatgtcgcgt gaggggttcg
    13261 caggcgatct cctcgatgcg atgaaccact cgcgcgattg cccgatccgg cgcgatggga
    13321 ctgtggttgg cgagaaccgc gctgtaatcg gggcggatcc cccgccggag tcgcaggagg
    13381 gcatttcgca gatcttcgct cccggataaa acttccttgg aattggtgcc gtcaaatatg
    13441 gttttcagtc cgaggggcac cagcaagacg gggattgagc gaagcaaagc ctcaaggata
    13501 acttgtgaat tgccacggtt gatacaggct gccgagattt ccaggacgtg gtggatgctg
    13561 agatcgggcg gggccacaat tacccgcggg ctgcacgact tcactgaccg gacgccggcg
    13621 tcgaagtcct cgcggggatg aagcttgact accgtgcgtt gcccgggaag cctggctgct
    13681 tccacggtgg tgatcaggtc gtgccgaaca cgctgggttt ccgtgctcgc aacggcgccc
    13741 gccccgtcgg aaagaagaga gagcgcaagc gtgatgactt cttcatcgcc ttggatcccc
    13801 agtcgcgccc gggtctctga ccgatgatcg atcccgtcat caagcaattg ggagtgaaag
    13861 ggccagcccg tgacctccac cacctcggcc ggcacgccca gtcccgtcaa ggcgtcggct
    13921 tcgtagggtg atgcgagaaa gagatgatcg atgggtacga tataattatt aatccttccc
    13981 tcggtcattg catgctggtt gacctcctca attgcaatgg cgggcagctt gtggcgaatg
    14041 agcgcggcca ggagaaggaa tatctccttg tcggggaaca ctttcgagaa gatggccacc
    14101 ttgatccgct gtcccgccgc aataagatag gagtcgatgt catccagatt gagccatgaa
    14161 aagccgttgc ccgcgggaca tgcggcaagg gattccgtgc gtgtcgagtg gcagatgacg
    14221 aagtcccact ccggcatatg ccgagacaag atgagaaggt tgcgcagatg cacgggacca
    14281 aacgggaggt aaagcgcaat gggcttcatc gtagccaatg atttttgaac gtggcccagg
    14341 cccagtaagc ccgaagttgg agtgaaggcc gttcacggcg taacggaaga tcgatgggcg
    14401 gaagtcgagg cagcggccga ttgcgggtca ggaagtggtg aaatcgggca aattcatcgc
    14461 gcttgaaggg tgcaagccgg atcgcctcgc gcacctgatc cagactggag caaacaggaa
    14521 tatcacagcc ggaccattcg atgagcgctt caatcttgtg agaattcgag ggcaagggga
    14581 cgaaggggat gccggcaagc cccgccagat aaacgccgtg gtgcctgccc gtaatataaa
    14641 tcgatgccgt tttcagattc gccacggcgt ggggccaatc caacttccga tcctcgtggt
    14701 ggaggtccca gctcgggtga tcgcgtttaa gtcgatccag aacttcaagg atatccgccc
    14761 gagaggggtg agcgtccccg atgacgactt gaccaccgaa gtcggcggat gcctgctcca
    14821 ggaatgccgc tgcaacgatc gaatcgggtg caatgcacgc atcgcggccg agccggcgag
    14881 cttcagcggc actcctcggt tcccgaacga ctagatagtc gagatttcga atcacatcgt
    14941 ggaaggcatt cagatcctcc agcagactgt tgatcagcag agttcgcttg ccgagccttt
    15001 gtgcggcgcc gagaaaagca agcagatgat tgccattgcc gtgatggatg gttccctcgc
    15061 cattgacaac aacggcatcg cattcgaaga tcgccctgcc ccatcgactt ttcaaggcag
    15121 ccgcaatcgc ttgattttcg tcgccgttcc aaatttcctt gccctgcccg acgtagtggc
    15181 gttggatcac gacagcgccg gcctcccaaa tcatgcgatc atgggcgtcg gtcaccgcca
    15241 gacatccgac gtgacataca tgccgcgtgt catttgccca gaagagcttc acgtggactt
    15301 ctccgccggg cattgagtcg gcgccacctc ccagtgggcg gagccgcaaa tgatcccggt
    15361 gccgaccgcc ggcggcgggc cctcgagggg tacgttcgta acatggaagc gagtgacatc
    15421 catcacgagg tccagggatt gacgtccttg gcgcgccatc agctttgaga ccttgtaatc
    15481 gccgggcatc aaatgcatgc ctcgcggttg gaatgagatg atgtatgtcc cgggaagcat
    15541 cgtgcctgga tgcagatcat tctcttcgct gtcactggtg tagacccgcg tgccgtccag
    15601 ccgatcgacc ccaagatgaa acgacagaga ggggaggggc tccaaaacct ccaagccaaa
    15661 agttactcgc aaaggttcac gatagcgaaa aaccgatgtc agttcgttat cccgattccg
    15721 aagctcgcag tagagcagcc tggccggccc ctgcgtggga gggttgcgga ttgtcaggtc
    15781 aacgcgtcct ccattttctg ctccgcccag ggtatcacgg atgtactgat cgaaaacttc
    15841 ggacgttctg ccccaggcac ggaccgcccc ggcgtcaagg taaatacttg tccggcagag
    15901 attctggatg gcgcccatat tgtggctgac gaacagaatg gtgcgaccgt catgagcggc
    15961 attttccatt ttgcccaggc acttcttttg aaactgcgcg tcgccgacgg ccaggacctc
    16021 gtccacgacc atgatgtcgg tgcgcaggtg ggccgccacc gaaaaggcga ggcgaacata
    16081 catccccgag ctgtagaact tgacgggcgt atcgaggaat tgttcgatcc cggcaaattc
    16141 aacgatggcg tcgaactgtt gggcaatctc ggcgcggcgc atgccgagga tggcgccgtt
    16201 gagatagatg ttttcgcggc cggtcagctc gcggtggaaa ccggtgccga cctccagcag
    16261 gctggccagt cgcccgcggt agcgcatttc gccggtcgtg ggcgcggtaa tccggctgag
    16321 gattttgagc agggtcgact tgcccgcgcc gttgcggcca atgatgccga cggtttcgcc
    16381 gcgggcgatc tccagattga tgtcgcggag cgcccagaaa gtccccttat cgccgcgacc
    16441 gcgtcgagcc aggccaaagc cgcggcgcag caggctcgtc agctcttcgc gaaaattatc
    16501 cttgagcgcc cgcgtctccc ccaggcgata ggccttgccg atatcccgca tgcgcaccgc
    16561 ccacggggtg gccgcttcgg cgccgggggt ggtttcggcg cgcctgacga tggcgggtgc
    16621 ttctgcgttg tccggcatgt cagaggacat ccacgagtcg gcgttctcga gccgcaaaga
    16681 agcgggcgcc gaagaaaagc accagggccg acatcacggc ggccttcagc agaagcatcg
    16741 agttgatcgg actgcgctcg atcaccgcgc gataggtttc gacaaccgcc gccatcggat
    16801 tgagcgaaag gacctgatac aggtaaggga cggcccaccc ccagtcaacc gagtggaatt
    16861 cgccgcgccc catggcttcc agcggcagcc agacggtgat gaacatgccc aactgcagca
    16921 cgaacgtgat cgcatatttc acatcccggt actgggcgtt caggatcgac agaaagagtc
    16981 ccaggccgac cgccgtggcg agttgaatga tcagcagcgg gatgagccac agcaagagcg
    17041 aggccgtgaa ggcgcccgcc agaaaggcaa tggccgccaa catcgcaaaa gcgaacagaa
    17101 aatcgagcag cgaagagagg acttgggatg ccggcacgat catgcgcggg aagtagattt
    17161 tcgcgatcag attggcattc tgctccaggc tggtggcggc catctgcgtg ccctgttgaa
    17221 aactcgtcca cggcgccagc ccggccatga aaaacagaag cgccgcggcg ccatcgaacg
    17281 agcctcgaat caagccatag atcgccgtga agacaacgcc cgtgatcaaa ggctgaagga
    17341 cgacccagat aattcccaag gcagtttgtt tatagcgtag tttgagatta cgaaggatga
    17401 aggccccaag cagctcgcga tactcgacaa gatcgcgaaa tgcctggatg agccgggtcc
    17461 gcggatcaga gcttataacg gtttcttgcg gattcatcgg ggcgtggggc gaggtttcct
    17521 aagcgattgg accgggcggc cccgcccctg gtcgtcgccg gaggcgccga acgggccagg
    17581 gcaggcaccg cagccaacct cagcttacgt caatccttgc agggtcatca atggtagacg
    17641 gcccaccaag cccataggcg cacagtgccg cgggctcctc gctgccgacg cgttcaacgc
    17701 ccgacccgcg aagcgccgtg cgtcgcctcg tcgtgttagt gagccgcggt ggctgccgcc
    17761 ggcgcggtaa tccccccctg ccgcgacatc cactctccgg cgcatgggcc ggcttttcac
    17821 ttcgcgcggc gcagcgcggc gacggcggcg gcggcctgtt cccggtcgtc gaagtgcgtc
    17881 ttgcgcccca tcgcgtcctg gtagttctcg tgccccttgc cggcgatgac gaccacgtcg
    17941 gccggcgtgg ccaggcggat cgcctcgcgg atggcgctgg cgcggtccag gatcacctgc
    18001 cagcgggtct tgccgaaggc ggcttcctta atcccatcca ggatcatctc ggcgatggcc
    18061 ttgggctcct cggtgcgcgg attgtcgttg gtcacgatga tgtggtcggc gagccgggcg
    18121 gcggcgcgtc ccatctgggg ccgcttgccg gggtcgcggt cgccccccgc gccgaaaacg
    18181 gcgatcagcc gccccgtccg gaaggcgcag gcattgagca gcgtctgctg cagcgaatcg
    18241 ggggtgtggg cataatcgac gatcacgggg aacggctggc cggcctcgac caattcaaac
    18301 cgccccgggg cgccgcgtgt cgcggccaga ccctgggcga tcgcgtcgtg atccagcccc
    18361 aggccaagcg ccatcccggc cgccaccagc gcgttctgca cattatacaa cccgtgcaac
    18421 cggagctgga ggtagaacgg ccgcccccgc aggcgcgccc ggacgtactg gccatggcgg
    18481 gtcacatgga ccgtatcgac aagcaggtcg gcgtcggggc ggtggaaact gaaggagaaa
    18541 acttgaacgc ccggtgcggt ctggcgcacg cgctcggcat agtgccgccc cgccggatcg
    18601 tccagattca gcaccgccac accgccgccc gccgccggca gcacctcggt gaacaatcgc
    18661 tccttcgcgg cgcggtattc gtccatcgtc ttgtggtagt ccaggtggtc ctgcgtcagg
    18721 ttggtgaacc cggcggcgcg gaactggatg ccgtccaccc ggtgctggtc aagggcatgg
    18781 gaactggttt ccatgacgag cgcctggacg ccctcggccc gcatccggcg caggtattcg
    18841 gccagttccg ccggcgaggg ggtcgtctga ggggcgggct cgagctccac atgatcgccc
    18901 cagcggtagt tgacggtgcc gaggacgccg gttttcaacc ccgccgcgag caggatggcc
    18961 tccagcatgt aggtgacggt cgtcttgcca ttggtgccgg tcacgcaggc ggccagcatg
    19021 ccggcgctgg gatcgcccgc cagtttctgg cagagccggg cataggccag gcgggtgttt
    19081 tcgacctgga tcaggtgcag cccggccggc gccttcgccc ggggagagct gacgagcgcg
    19141 gcaggtgcgc cggccgcggc ggcttgcgcc agaaactggt ggccgtcggc ccggccgccg
    19201 gcgatggcga caaagagcac ccccgggccg cagcggcgcg agtcatcggt caccgcctca
    19261 atcgacaggt cggccggcac gcgcgcggcc ggcgcgagcc gggtctcttc aagcaatgcg
    19321 gccaggttgt gcatgggaag gcttgttgtg gtcctcggcg gggaatggat ttccgcccgc
    19381 ccaaccgatt cggcggcgct cgcctgtggc tccggaaccg accgggagag cggccctatt
    19441 gtgcctgaaa ggcgtcctcg cccgcactgg ctttctgggc cacgtcggta ttctgggcaa
    19501 acaccaccat cgccgcgcgg ctcgccggga ggcggcggcc cggggctgga tcctggcgca
    19561 cggccagccc cgagccgatc atgcgcgcct cgatgccggc ttcggccagc cgctcccagg
    19621 cttccaccat ggtcagcccc aggcaatcgg gcatgcgcgg ccgttcagcc tcggcgtctt
    19681 cgtcgccggt tgcgggcagc aggctctcgg ccagcgccag atccaccgcc ggcagaaccg
    19741 ggcggcccgg ctcgaccggc agcgcggcgg gcatgccgcc cgaatcggcg tcgaccgcca
    19801 gcacgccatt atcctcggcc gacggcgagg agtcgccgtc ctcagcgacc cgcagcgccg
    19861 gatcgacaaa cccctcctcc ttgcggcgat aggcttcata ctcggcgggg tcggaggggg
    19921 ggatgccgag gatatgggcg accttcagcg cgatctcgcg gaagaccggc gcggagacct
    19981 cgccgccgta tttggcgccg cgcggctcgt ccacggtgac gaaaatcgcc acgcgcggct
    20041 cgtcgatcgg aaacagcccg gcgaacgagg cgatgtagag cttgcggccc gaatccgacc
    20101 ggtagtgctt gacggtcgtg ccggttttgc cgccgacgcg gaagccgggc acctcggcgc
    20161 ggcccttgcc ggtcccctcc agcacgaccg cctccatcag ctccagcatg ttggcacagg
    20221 tctcggggtt ggccacgcgt tcgaccggtt cgccggggaa ctcgcggatc gtgcgcccgt
    20281 cgcgcgagac gaccgcctgc accacgtggg gggccattcg ccagccgttg ttgccgatcg
    20341 ccgccagcgc cgagaccgcc tggagcggcg tcagtgaggt ttcgtagcca atcgccagcg
    20401 aggcgcgcga catcgccgtc cattgctcga ccggccgcag gatgccgctc ccctcgcccg
    20461 gcaggtcgat tcccagcgag ctgcccagtc caaaccggcg caggccctgg tagtaggcca
    20521 cctgctcgat ccgctcgctc aacagcgcca tcgcgacgtt gctcgattcg gcgaaggcat
    20581 ggcgcaacgg gacccagccg ccgatcgcat gcacgtcgcg gatccaccgg ccatgcagga
    20641 aaaccgcccc cgagccggtg ttgacgttct cgctgggcgt cagcaggccg ccatccagca
    20701 acaacgtcgt cgtcaggatt ttcatcaccg agccgacctc gaacgggtca gtcagcgccc
    20761 ggttcagccg ctgatcgtcg tcggtctcgg agaagtggtt ggggtcgaaa tcggggcagt
    20821 tggccagggc gaggatgccg ccggtgcgca cgtccatcac gatggcgacg ccgctgcgcg
    20881 cctgcagctc gccgacccgc cgccgcagcg cccgctgcgt aaacatctgg atctgctcgt
    20941 cgatcgtcag aatcagattg tggccgtagg tcgattcaat cacctcgtcg gcgatcggcg
    21001 ccaggccggt ccgcagcgaa ttgaccggta tctgcgtctc ctgataagac cccttcaggt
    21061 actcgtcata ctgcagttcg acgccggcca ggccgatgtt gtccccggcg tcgtcgatct
    21121 tcgtgaagcc caggatgtgg gcggccaggt gcgccttggg gtaatagcgc ttgctctgtt
    21181 tctcaaaaat gatcgtattg ggcggcaggt catagcgctc gtcgatcatc gccatcgcct
    21241 ggctgacctc ttcgggcgcc ttgcggtaga cgtagtagcg atacggcttg tcgaaatact
    21301 gctggatctg gctgcgcggg cggcccagca cctcggccag atcgtcgatc aggcccggcg
    21361 ggatgtccag gggagcctcg tcgcccttgc cgcggcgcag cttgcgtgta tccagcacga
    21421 tcgtatcagt gaagtacgag gtggccagga tgctgccgtt gcggtcgagg atgtcgccgc
    21481 gccggggcgt cagcgtcacc cgtttgtgct gctgctcggc ggcccggacc agatatttct
    21541 cgttgtcgat gacctggacg tgatagaggc ggaccaggat ggcggagaag acgaagaacg
    21601 tgctgcacca gagcagcagc agccggaagc ggtaggtctt acggagcatg gggggcctcc
    21661 aggcgggcta tgtccaatcc cgggaacggt ccgcaagccc gatgaggaac cggccgatga
    21721 agcgctcagg gggtgcggcc ggcgaccgcc ccccgttcgt tttggtcggc cgatgcgggg
    21781 gacgaggggg cccgcggccc gccaaataca atttgacaac ccggaagggg ctggataccg
    21841 tcgatctcag gttcagtggc gagtcggccc gattccgggc aaaaaaccta ccatctcgtt
    21901 ccggcgccga acggcgcggg aactgtgcgc caggcggggg gaacgtcgtg agcggtcgga
    21961 tgccgcgacg ttgcccccct tctgtttttg ggccggcgag gcccgcaaca gtgcctggcg
    22021 tctccgcact cgtccgccct gcgccgcccg cgcttggtcg ggaagggagg tcttgaacag
    22081 gcaacgcggc tgggagcggc aacatggacc gacgtcacgg ggagagcgat cgagcagcgg
    22141 caacggagca gccggagatc cacgggagca gaacagcggg aacgggatcg atcgcctggg
    22201 tcggcacccg gtgcgggaat acggtccggt cagggggaac gcgcacggga aacagctgcg
    22261 ggagatgaac agcgggagcg ggacgcgcgc ggccacggac ggcctgagtg ggagaaactc
    22321 gaagccggcg gccgcctggg cggcgccggt gaaatctgtc atcgcgccct ggtcggcgcg
    22381 gggccatcct ccatcgttcg tcctggctcg aaaattcgtt gccggggctc ggttcccggc
    22441 tttcgaaaat ctcctgattt gcgcgaaggg atgagtcacg cctgagcggc cctccccgcg
    22501 ttcttcaagt cctgatcgcc ctcattcccc ggcctgggcc gcgggcgccc agccgacgcg
    22561 ttctcccagc cgctccagcc agagggcggc ctggatcgcc gtcggcgaga gcggcggttg
    22621 cgccgggccg ccggccttgg tccgcgccat cgcgtagcgc gtgtagatct cctcgggcat
    22681 ccggatctcg acgcgctcct cgccgtaggg catcatcccc agttcggcgc gggcaaattc
    22741 gaacagccgc tcgggctgtt tcagcgcctc cagttcaccg cgcagggcgt tgttctcgct
    22801 cgtcagctgg tcatgcgtcg tctgcaggcg gatcgcgtca cgccgcaccc cgtcgagcat
    22861 gaagcgcagg tgcaggtggc ccaacgcgaa cagcagcagc agccccagcg caaacaggac
    22921 gcgcatcaac gcggagaacg gcaccagccg cacattgtcg ccgggatcga gtcgctgcgc
    22981 gtatccagcc aatgctccgt tctcctttcc cgtccgaaaa tcccgtcgct cgtcttcgtg
    23041 atcgccgctg cggcgtcaat gcgtgggccc gaaatcagcc cgccgtctcg tccccgagtt
    23101 tctcgatcgc ccgcagtttc gacgatcggc ttctggggtt gtgccgcagt tcgtcctcgc
    23161 gcggcgtcag cggcttcctc gtcagcagcc gggccggcgc ctgggccgcg ccttgcggca
    23221 acggccacgg cgggagctcg gcggtcgact tctcccgcat gaactgtttg acgatccggt
    23281 cttcacctcc gtggaacgtg atcacgacca gccggccgcc cggcttgagg gcctcgaagg
    23341 ccgccggcag cacgcgctgg agcgagcgat attcgccgtt gacctcgatc cgcagcgcca
    23401 ggaaaacccg tgtggccggg tgaatcctgg gcggccaggc cttgcgcggg atcgcggccg
    23461 cgacaatctc gcccaaccgc cggccgctct cgatcggccc cttgacccgc tcggccgcga
    23521 tccgctgggc gattggcccg gcccagcgct cgtcggcata ctcgcggaag atccgcgcca
    23581 gttccttcgg cggccaggag ttgacgatct cggcggcggt cggcccgccc gcccgcgggt
    23641 tcattcgcat gtcgagcgag gggctcgtcc aggacaggcc gcggtcgggg tcgagcaact
    23701 ggggcgtgga ggggccgagg tcgagcagca cggcatcggg agcgggaaca tcggccagtt
    23761 gcggatcgtg ccggatcagt tcggccagat cctcatggtt cgccaggagc aggcgatagc
    23821 gggagcgggg gcaaccggcg gcctcgaggc gccgccgggc ttcggcaagc atctgcggat
    23881 cgcgttccac accgaccagc aacccctcct cgcccagtcg cgcgagaatc agtcggctat
    23941 gtccgcccag ccccagcgtc aggtcaaaca ccgttttgcc gggggccggg tcgagggtct
    24001 ggagcacctc ctcctgcaag accggttcgt gatcgaccgg ctcgctcatc gtcggctcag
    24061 gcgccggggg cgttcgggcc cgcgccgggc acgttcagaa caaactggtt gacgggggcg
    24121 gccggcgcct tgcgggccgc gatcgccgtc gtcgccttgc cgtaaacctt caatccggcg
    24181 ttcaccatct ggcgccactt gttgacgtcg aagacctgca tgtacttcag gctgccgaca
    24241 aagacgacct gccggtcaat cgagcagaac tccatcatca gcgggttgag cttgatccgg
    24301 ttctgcttgt ccagcggcag cagctcggcg gtcgaggtcg ccaggttcag caccatccgc
    24361 tcgtcgtcga cctggtcgtc gagctggctg agccgctcga tctcctgctc gaagtagttg
    24421 gccggcatca cgcggatcga ttcgtcgggc gtgatcatga cgccgacctg ctcggtctgc
    24481 tccgggcaga cggcgcgcag gacggccatg aaacgcgcgg ggatgcccac ccgtcccttg
    24541 ccgtcgacgg tcatctgctc gttgccgagg aaccgggtct ccatcgggac ctcattcagg
    24601 gccgggaagg gaaccgactg ggccgggttc cccaggagtg tccaggatta gggcggattc
    24661 cccaaaattc aggtcctgtt ccacatccag ccctactttt ccccacgttt gtacacacac
    24721 tcccccgggc ggtcaagcac ttattttgga cctgccagcc cgagtcggat cggatgagga
    24781 atccgcccat gttcacgtgc gacggtggag gaagggccgg gccatcctgc gccagggatt
    24841 gcgtcagaat gacgcagaat tgtggccggt cggctccagg ttgctatcgc gatgcccctc
    24901 gcgtcggcgg atcggatctg gaggcgggtg gccgactcac ggcgccgcga gccggttccc
    24961 gtgtcgcgcg catggcgctt ccggggcggg ccggcccacc gtcgcggccc gagctcaggt
    25021 ccgttgcgtg gccgatggcg gagtgcggcc gacccgtggc acgcatgttg caccggcctt
    25081 gacaacccgg agggatgtgt tatctatcgg agcgattccg ccccaggttg tggcaacccg
    25141 cttaggatgg aggtagcggc agatggtcga gccagcagcg gtcggcaaca gcgccgttcc
    25201 cgaaaccggc gcgccggtca tgatcgaggt cagtaatctg accaagctct atggcctgac
    25261 ccgcgcggtg gacaatatct cgttcacggt cggcaagggc gagatcctcg gctttctcgg
    25321 cccaaacggg gcgggcaaga cgacaaccat gcgggtgctg acgtgctaca cgccggcgtc
    25381 ggcggggacg gcgcgcatcg gcggctacga cgtctcgcgc cagtccgaac aggcgcgctc
    25441 gatcctgggc tacctgccgg aaaacgctcc gctgtaccag gacatgctgg tggatgacta
    25501 tctgcggttc atggccgagg tgaagcgcta tccgggcagc cggcggcgcg ccatcgttga
    25561 ggaagcgatg gaggaatgcg gcctgaccga cgtgcgcaag cggatcatcc gcaacctgtc
    25621 gaagggttac cgccagcggg tggggctggc gcaggcgctg ctgggcgatc cacaggtgct
    25681 ggtgctggac gaaccgacgg tagggctgga cccgcggcag atcgccgaga tccgccagct
    25741 catcaagggg atggccggac ggcggacggt gatcctgtcg acgcacatcc tgccggaagt
    25801 ctcgatgacc tgccagaagg tgatcatcat caaccagggt cggattgaag cgcaggggac
    25861 gcccgaaagc ctggtctcgg cactggaagc ggccaatgtg gtgatggtga cggtcgacgg
    25921 cccggcggcg gcggtgcagg aactgatcag ccgggtgccg ggggtcaaga gcgtcgcgca
    25981 ggagcgcatg ctggggggct caacggcggt ctaccgcgtc gaggccgcgc cgcaggggga
    26041 cccgcggccc gagctggccc aggcgatcat cggcggcggc taccggctgt ttgagctgca
    26101 gtccagcggc tattcgctgg aggacatttt cctgcggatc atctcgcggc agaaggaggc
    26161 ggcctgatga acttcctgcc gatctaccgg cgcgaactga agacctactt ccagtcgccc
    26221 agcacctaca tcgtgatggc gctgctgttc ctggtgctgg ggatgatcta ttcgcagatc
    26281 atggtcctgt tcagcaacat gtcggcgcag gccggcatgc cgaacatgtt cggccagacg
    26341 ccgcccgagc cgaacgtgac ggagatggtc atccgcagtc tgtttcagat catgatcgcg
    26401 ctgatcctgt tcacggtgcc gctgctgtcg atgcggctgg tggccgagga aaagagccgc
    26461 gggacattcg agctgctggt cacgtgcccg atcagcgact gggcgattct gatgggcaag
    26521 tatctggcgc tggtgacggt cggggtgatc gtgatcctgc tcagcggcct gtttccggtg
    26581 attacctggt gggcggggcg cggcagcgaa gtggtgatcg aatggccgat cgtgctgtcg
    26641 tgctggctcg gcctgctgct catattcgcg gcctacgccg cgttcggcgt gatggcctcg
    26701 tccttttccg aaaaccagat cacggcgggg gtgatcacgc tcatcgggct gatcatgtgg
    26761 aacatgctgg cgagtttcca gatcgagagt tttccccgga tccagcagat tttcaccgaa
    26821 ctggcggccg agccgcacac gacgaatttc gtcaagggac tgctgaccac caaggacgtg
    26881 gcgttctact tcctggcttc cttcctgttc ctgttcatag ccagcaagac gctcgacgcg
    26941 cggcgctgga gggtgtgatc gatgcatgac aagacgttga agatctggtt tcgaacgctg
    27001 ccggtcatcg gcgtcctgtt cgtgatcgcc ggtttcggct gggcgatccg gcaggcggcc
    27061 gtcgacccgg tcgtctatac gctggtcggc atcggcctgg cgctgttcct gacgcttttc
    27121 gccaaggcgg agctggccaa cctgaagtat tacctccacg tcgtcgttta ctcactactg
    27181 gtcttcatcc tctgcgcgat cgtctatctg gcaatccggc agtacgacaa gcaggccgac
    27241 ctgaccgaac agaatctcta tacgctctcc gaccagtcgc gccaggtgct ggatcaactg
    27301 aaggaaccag tcgagatcgt cttcttcttc agcaccgatc agccgttccg cgacatcatg
    27361 cgcctgtatg gggacgcctc ggaccagatc cagttcaagg tcatcgatcc catcaaggat
    27421 ccggtggcgg cgatgcgcta cggcgaaaat attcgcaccg gcgaggtagt gatccgcaag
    27481 gttcccgaga aaggcgaggg gacgcccgcg ccggcggcgg agaccaaaca gaagcggatt
    27541 tcggcgactg agtttggcca gggtttcacg cagatgggcg tgcaggccga gaatgccctg
    27601 accaatgcga tcatcgaagt gacgcgcgcc gagacgatca aactgtattt cctggagggc
    27661 cacggcgagg tgtcgtatga cgaggcgccg atgccgcagc ccggccagcg ctccagcgtc
    27721 gcctcgctct cggcgctgcg caagttcctg gaagacccgg gaatggagac ggcgcggctt
    27781 aacctgatca cgagcggcct ggtgccggag gatgcgacga tggtcgtgat cgccgggccg
    27841 gcgggcgacc tgctgccgcc ggagatctcg gcgctgcgcg actacctgac acagcgctac
    27901 ggcaaactga tggtgctgct cgatgttccg caggagaatt tcgcgctgcg cctgcccaac
    27961 ctgctggggc tgctggagga gtacggcgtc agctcgcccg acgaggtaat catcgatctg
    28021 gccaatccgt tcagtccggt gcagccgatt gtgcaggatt atgccgagga tcatccgatc
    28081 acgcgggacc tgcgccaggc gatgatgctt tcgatctcgc ggccggtcgt cagcaggatg
    28141 caggggggcg ggcagtggca ggtgaccgag ctgatgcgga cgtcgcccag ttcgttctcc
    28201 aaggacttgc aggaggtggt ggccaacgag cggatcacgc ccccgccgcg cagcgagatg
    28261 cagccgcaga cgctggcggt ggcggtcagt ccgcggacgc cgccgcagat gcccaacatg
    28321 ccgccgctgg aggagcccga gggcgcccgc ctggtggtgt tcggcacgag cgagttcgtg
    28381 cagaattcga cgctgacgca gtccaacgtg gccgtcaacc tgatgctcaa cacgatcaac
    28441 tggctggccg agcaggagga tgtgattgcg atcccgccgc gccagctgga ggggacgccg
    28501 atcatcctga cgccgcagca actgcgggtg atcttcgtgc tggccgtcat cctgttgccc
    28561 ggcgcgttgt tcttcggcgg cgtgagctac tcgatcgccc ggcggcggcg gtaggcggcc
    28621 agcgagccga gacggcccgg cgggttttcg agagccgggg acgagttgct catgaaccgg
    28681 cggttctcaa cgaggaatga aaagggaggg caacgccact ccggagcgtg gcgctcccag
    28741 gtttcggccg gtaatgaacg gacgagggat gcgtggttct cgcgaagggg aacgctccgc
    28801 gcccgttccc tgaattcatg gagagtgcat cgatgagctg gaaacggact gtcattgtcg
    28861 ccgtgatcgc cgtgctggcg ggggcgtggt atgtctggga tcagaagcgg atcgaggaga
    28921 aggaggcggt cgaaacggtc gcctcgcggc tggtcaccca ggcgaaggag gcgtttcagt
    28981 cggtggaact ggaacgcgcc gcagccggcg acgtgcaact cgtgcgcgag gacgagacct
    29041 ggcgcctgac ggaaccggtg gcgacgcgcg ccgatgagac ggccgtcaac agcctgatcg
    29101 acgccatcga ttcgtccagg aaggaaaata cctttgaagt ggccgatgga aatttcgagg
    29161 ccttcggcct ggcggagccg gaaatcaagg ttcaggttca ggccggcgaa gggcagatca
    29221 ccgcgtttga actcggcaac cggacgaccg acgacagcgc ggtgtatgcg cgcatggccg
    29281 gcggcaagga ggtcttcacc gtcccgctca acctggaaac gcagctctcc aagtcgttca
    29341 ccgacctgcg cgacaagcgc gtgctgccgg ccaacctgaa cgaagcgacg acggtgaccc
    29401 tgcagttcga gatgacgacg ctgacggccg agaaatccga tgggaagtgg aggctgcgcg
    29461 agccgatcgc gcaaccggcc gattcggcga agatgagcga gatcctgcgc aactggaaca
    29521 atgcgcgggc cagcgacttc atcgacagcg atacgctgaa cctggcggat ttcggcctgg
    29581 agcccccggc gctgcgcgcg atcgcctatg tcgccgacga ggcgacgacg gccggccgcc
    29641 tgctcgccat gcgcatcggc gacaccccca ccaccggcgc ggccaagcgc tgggcgatgg
    29701 ccgagggcga agggggaatc ttccaggttc ccgccacgct gctgacccag ttgcagcccg
    29761 acctgcgcga cctgcgctcc aaggaaatct tctcgatgac cagtgccgag gtgggcaagg
    29821 cgatcttcaa cgtgaacggg caggtgctgg cgctggagcg ggactcggcc gacgtctggc
    29881 atctggccga tgatccggac gccaagctgg atcaggggac cgtcggcgcc aaggtgagca
    29941 gtctgaccaa cctgcaggtg aaggaatacc tggaacttcc gcccgatccg gccgagacgg
    30001 ggctggacag ccccaacctg atcgcgacgc tggtcacgcg cgacggcgcc acgtcgcaga
    30061 cggtgatcac gggccgcaag gccggcgacg cggactatgt ctatgcgcgc cgcggcgacg
    30121 atccggagat cttcggcgtg gactggaccc gtccgggcga cttcttcctg acgcgcgacg
    30181 acgtgatcga caggagcatc ttcgatttcg atcagtccct ggtgcagacc ctccggatcc
    30241 gcgacggcga gcgcgagctg acgctgaagc ggaccgacag cggcgcatgg aatgtcttcc
    30301 gcggcgagga ggacgaccgg atcggccagg tggaggggag ctacgtcacg ccgctgctct
    30361 atgcggtcac cgggctggat tgggacttgc agttcgacgc cggagactcc gaggtgcagc
    30421 agtatgatct gattaccccc tcgcgcgagc tgacgatgct cgacgaggaa gatgtcgagc
    30481 tgggccggct cgggcagggc gaatcgagcg atatctacgt gcatctggtc cgcggcggca
    30541 atgcgttctt cgccgtcggg atggacgatt acgcgtcggt cagtgattcc ttgcagtcgc
    30601 tgctggatca gatcgagctg aagtaaaccc ggggccggct tgcgccgcga gcgagaggat
    30661 tctggaccac ggcggtcagc cgcggacggc gcggttaatc tcggcctcat cggtttcgcc
    30721 ggccgccgcg aggcggcgtg ccgcctcggc cagcatgacc atcccaccgg cctggcaggc
    30781 ccggcgcagc tcggcctctt ccagcggacg gcgcgccgca tcccacaccc cggcatccgg
    30841 ttccagccac tcaaagacgg cgcgacgccc ctgatagccg gcgccgcggc agacgtcgca
    30901 ttccgccgac gggccatgcg cgccggcagg cggtcggcgg cacgcggggc agagccggcg
    30961 caccagccgc tggctgacga tgccgatcag atcctcgcgg agttgccacc agggcaggcc
    31021 gatgtcgagc agcagccgga gcgcttcgac cgacccgggg gagtgcatca gagcgacgac
    31081 gagccggccg gcggccgcgg cgcgcagcgc catgcgcaac gcgtcgggct cctcgggcgc
    31141 atcgatcagc agcacgtcgg gttcgagccg cagcgcggcg tggatgcgcg cggggagcgg
    31201 ctcgccgctg gggagcgcct cgagctggac ggcgccgtcg aagcgaagct gcaccggcga
    31261 ctcaacagtc acgagcttgc gaccgcccgc ggccaggaga tgatgcgcga gggcatgcag
    31321 cagcgtgctg cggccgctgc cgccgggacc ggtgaccagg atcagcccct ggggccgctc
    31381 gatccagcgg gcgaaccgcc gccggtcggc gtcctcaatc aggatctgat ccaatcggag
    31441 cagatcgcgc tcggggcgga acaggcgcac gaccatcgcc tcccccgcgc tggagcgcag
    31501 cccgctgagg cgcagatcga ccggctcgcg gtcgaggagc agccggaagt gtccctcgat
    31561 cagcggcggc gcattcatca aatcgacatc ggccatcttc ttcaggcgag ccaggacggc
    31621 gcggtagatt tcgggttcca agcgcgattc ctcgaccagc accccgtcga tgcgcagatg
    31681 gacggcgccg gcgtcggcgg cgggcgtcag atgaacagcc gcggcgccgc gggcgagcgc
    31741 ccgccgcagc agcgactcgg tcgccgcgcg tgcctcgggc tggcgcgcca gctccgcctc
    31801 cgaggccggc tgttcctcct cgctcaggcc gatgcgcccc tcgcgcacgg cttcggggat
    31861 ctgcagcagc acctcctcaa gcaggccgct gttttcgccg gcgcggatca tcacgaccag
    31921 cgaccgcggg aaaatctcgc ccatttcgct cagcgtgtcg ctgatcatct ggccgtcgcg
    31981 ggcgccgcgc gcgatgcgga aaaacgcgtc gcgcagaacc ggatcgtccg attcttcggc
    32041 aatgacttca atcgattcga tgatcggcgc gccgctgcgc agcacgcggc ccagcttgcc
    32101 ccacatgacg gactgtttga tcatctgttc gcggcgcatg gtcggccttt gtcgaatcca
    32161 gcgactgccc accattttcc acaggcgcgc gtcaagcgga agcgcgaaga aggcggctcg
    32221 aatcagctta atcgctccac ggcgaaggcc ttcgccggat tgtggatgat cagttggtcg
    32281 atctccggct gactgaaccc gcgctccctc agcaacggga tgaaatccgt gaacagcgtg
    32341 tcgtggccgc gataggcccc gccgcccggt tcgccgacgg tgtaccagcc ggcgtcgtgg
    32401 gagagcagca cacgccccag caggccggcc tcgcgcaatg cgagcatcag ggtgagatgt
    32461 tggtccaggg tgtcggggcc gatgccgtcc agctcgaccc atgcgccggc gcgcgccgct
    32521 tccagcaggc ggtcggggtt cggctcgctc tgggcatgaa cccagatgaa gcgctcgatg
    32581 gcggccgctt cggcgcgcag cacgtcgagt tcctccagcg cggcgatgcc gtcgcccgtg
    32641 tgggcggcga tggtcagacc cgttgcccgt tgcgcgcggg cggcggccgc cacgagcttg
    32701 cgatggacgt cggaaagcgg cccggcgtcg acgccgatct tgatgaagcc gggccggatg
    32761 ccggtctggc cgatcccgtc gcgccattcg gcgatccagc gcgcggccag ttgatcggcc
    32821 gattcagtca gcgcatgggc cggcagatat ttgttcgccc cggcgccgta gtaaccggtg
    32881 ttggtcacga gtcgcaggcc ggtcgccgcc gcgagccggc gcagcagttc cgggtcgcgc
    32941 ccgatccagg cgggggtgca ttcgcagatc gtgcgcgccc ccagggcctg cacgcgctcc
    33001 agatgcggcc ggaccgcgga aaagacttcg tcggcgtcgt agcgcgacga atcgacctga
    33061 tcggcgccga tgaaatcaac catcacgtgt tcgtgcggga gcatgatgcc cgcttccgcg
    33121 gcggggatcg gtccctcgac ggtgatgatc caaccggtcg ggagggccgg ctctggcggg
    33181 atcgaggcgg tggcgctggt cagggcatcg ttttccattg cggcgcgccc gaccgcgccg
    33241 gccgcgagca gggccaggcc cgatttgagg atggccctgc gtttcagtga aacggcgtac
    33301 cggcggtctc ccatgacaat gcctccctca ctttggaatc gtccctcgtc ctcgtgctca
    33361 ctcgagaatt tgtccgctat tctcgggaac gcttggatcg gttctgccgt cccaacggga
    33421 ctctgtcacg atgggatccc ggttcccgcc agtaaactgg cgggctgaag tctggcgtcc
    33481 ctgacgggac tgccctgctg aagaatccgg caaggccaag gctgtttgaa tcggccgttc
    33541 ctccccattt tcatccctcg ctgtgaaccg caggttcatg agcaaacttg tgatcccccc
    33601 ccaaaggcct acccaaaatt cggctctcgg cgctcgaaaa ttcgtgaatg aattcgatac
    33661 cgatcccgat accgatcgcg aatatcaggg agggaggcgg gggatgaggg cttgcagcag
    33721 ccgcagcaac cgccggccgg cccgctcgcc gacttccagc acctcctcat gagttgtcgg
    33781 cccctgcggc cggacgtgcg aattcgtgat cagcgagatc gccgccacgc gcattccggc
    33841 gtggcgcgcg gcgatgactt ccggaaccgt ggacatgccg acggcgtcgg cgccaagcgt
    33901 ccgccacaac cgcgcctcgg accgcgtttc atacgtcggt cccgccagcg ccacgtagac
    33961 cccccgcttc aggtcgatgc cctgttcgcg cgcggtctcg tcgatcagct cgcccatctc
    34021 gcggtcgtag ggttcgctca tgtcgggaaa gcgcgggccg ttgcgttcat cgttcggtcc
    34081 gatcagcggg ttgcggtgct ggccgttgat gtggtcggtg acgcgcatca gatcgccgac
    34141 gcgcaggcgg ggcgagagtc cgccggccgc gttggtcaga atcagccgcg agacgccgag
    34201 ccgcgccagc agccggacgc cggcgatcac ctgcgattgc gcgacgccct cgtagaaatg
    34261 ccgccgcatc cgcaacacat gcgcgcgccg gccgccgagc cgcacgatat gccaccgtcc
    34321 gccgtggccc gccacggccc cctggccgac ggcgggcagt tcggaaaagg aaatgctctc
    34381 ctcatcctcg ccgatctcga ccagccgcga cagccccgac ccagtgacaa tggcgaggtc
    34441 cgccgtttcc ggctcgggca gcccgcggcg cagcgcggcc aggctctgtt cgaacagcgt
    34501 gttgttcatg cggatccccg cggcgggacg cccgatatga gagagcgcgc gcaccgccgc
    34561 tcgcgcggac gcgggcgtgg aatgtcaggc tcccaaactt tccttccgtc gcgcggttcc
    34621 cagtgggcct ctccgccgat caacccacca gcagccgcgg ggcgaagatc gtgccgcgca
    34681 gatacaggat ccagctcgtc aggaagtaga ccagcaggat cagccacatc atcgtgaaga
    34741 cgacggcgcg cgtcgtggcg cggccgaccc cctcggcgcc gcctccggcg gtatagccgt
    34801 aatagcagcc gcaaatcgcc atcgccgcgg cgaagaagaa cgtcatgatc aggccgctga
    34861 tcacgtcgcc cagcgtcatg aagtccagcg tgatctgaag gtactgattg gccgggacgc
    34921 cgagcgcgaa gacccccatg gcccaccccc cggccagccc gacgacgtcg gcgtagaccg
    34981 tcatcaccgg cagcatgatc atcacggcca gcagccgcgg cacgaccaga tagcgcatcg
    35041 ggttgacgtc gagcacttcc atcgcgtcga tctgttcggt cacgcgcatc gtgcccagtt
    35101 cggcggcgat cgaggcggcg gtgcgcgccc cgatcaccag cgccgtgaag acggggatca
    35161 tcacctgggt caggcccttg gcggcgccgg cggcggcata ctgtttggcg ttgaacatcg
    35221 ccagctcgtg tcccgtttgc aggacgatca ccatgccgac gaacagcgcg gtggtgaagg
    35281 ccagcggcag agcgttcatt ccgagctgat tggcatgcgc catgacgagc cgataccgga
    35341 tggggcgaaa gcacaggagg aaggtcttgg cggtaaagtg gaagaaatcg ccgaggtgcc
    35401 gcagcgtgtc ggtgatgtaa ttgccggtgc gcttcagcgc gatcgagccc gtcatctctc
    35461 gtccctcact ccaaaatcgt ctctcgctcg aaaatctgtc tccgatcccg ggatcgcctg
    35521 gatcggttct gtcgtcccta cgggactcgg tcgcgatggt gatcccggtt cccgccactg
    35581 acgtggcggg ctgaagtctg ccgttcctga cgggactgcc ctgctgttag atccggcgag
    35641 gcctggatca ttcgaatcgg ccgttcaccc tccccatatt catccctcgt tgtgaacggc
    35701 aggttcatga gcaactcgtc cccgtcctcg tgattgtgcc ccagagcccg tcccacgcat
    35761 taaacaccat cggcgcggcc gccgacagcg ggaagatggt tgggcggcgc ggcgcggcgc
    35821 gccgcgggcc gcggaaagcg cgaagattga agttgacggc gcggcccgcg gtgattatcg
    35881 ttacgaacaa atatgaacat aaaagaacag ggacgccatg gccacgcaac aggacgcagg
    35941 agcactcaca aacccattcc ggccggtccg agcctcgctg gcggacctcc tgggagatga
    36001 atacatcgag gcggtctgcg cgggccgggc ctggctggcg ggcgaggacc tcgacgcgct
    36061 acgggaggaa gcccggcgcc cggtggattt cctgccggcg gcgttcatcg agcgccagca
    36121 tgaactgctg ggccacaccg gccagagcgt tcccgccgcc gtgctgacct cgagtccggc
    36181 cggaagatcg accgaccggt tcaatgaggc gaccaagacg cacgtcgcgc cgctgacggg
    36241 gttcggtctg ttccggctgg ccgaaagcgg ccggctccac ctgatcacga agagcgagca
    36301 ttaccacgcc ctgttggggc attcgtttcc gggttaccgg ctgatcgacc gcgcccgggc
    36361 gctggggatc cccaatgcga cgcacaacaa cacgcgcggg tatatcacgc ggttgctgga
    36421 ggagcggctg gtggccgcgg ccaacgggac acccggcgcg gatgcggaca agttggatct
    36481 gaaccgcgtg ctgaacctgg agacgggaag cctggcggtg gaagcggcgc tcaagatgat
    36541 cctggcccgg ttccaccgga tccagggcga cgccgacccg ccggcgtatg aaggccgcat
    36601 tccggtcgtg ttggtgctgg gcaatgagaa cgggggtctg gaggccaatt accacggcac
    36661 gacgctgctg acgcagacga tgcgcgggat gtgggccagc ctgctggacc ggctgcaggc
    36721 ggcggggatt ttcgaggtcc gggcgatccg ccccaaccgg ctggaggacg tggaagcggc
    36781 gttcgccgaa tgcgagggcg nnnnnnnnnn gggcggcgcg cggaaaatcg ccgccttttt
    36841 ccatgaaatc gtgatgatga actacggcgc ggtgctgctg gagcgcgagt ttctgcggcg
    36901 gacatatgaa ctgtgccgcg cccacgatgt cccgacgatc gtcgatgaga tccagtcctg
    36961 catgtggtcg ccggagtttt tcctgttccg cgaatacggg ctgcggccga cgatggcggc
    37021 cgtcggcaag ggcttccctg gcggcgaata ccccgcgtcg cgcattctgt tcaatgcgag
    37081 catggacggg cggatgccgc agttcgcggc gctcgtgacg aacgggcagg aggagctggc
    37141 cgcgctggcc tacctgatca cgatgcactg ggcggcggcc aacgccgacg tgacgcgggc
    37201 catcggcgag tattacgagg agcggctgcg cgaattcgcc gagcgccacg ccgaccgggt
    37261 gaagggcatc aacgggcttc gccacatgtg cggactggag ttcgattcgc tggcggccgc
    37321 ggcggatttc gccgcgagca tggtccggat gggcttcgac atcagcgcgc aaacctacaa
    37381 ggtggactgc ccgccggtgg tgctgaccaa gctgccgctg atcgtgacgc gcgcggcggt
    37441 ggatttcatc ctggcccgga tggagcaggc aatcaccgaa tcggccgcgc cgcggacacc
    37501 gcggagcgct cacgcctgag tcacggaacg ggggatcgcc acgtgctgat cggcattccg
    37561 cgggagatca aggtcaagga gaaccgggtg gcctgcacac cgggaggcgt gcggctgctg
    37621 gcgggggccg gccaccgggt gctggtggaa cgcggcgccg gcgaagggag cggcttcagc
    37681 gacgagcagt atgggcaggc gggagccgag gtcgtggcga gcgcggcgga ggcttggtcg
    37741 gccgaactgg tgatcaaggt caaggagccg gtccccgcgg aatatcagta tctgcgcccc
    37801 gggctcgtcc tgttcacgta tctgcatctg gcggcggcgc ccgagttgac ccaggcgctg
    37861 ctcgacagcg ggacgaccgg catcgcctac gaaacggttg ccgtgggcgg gcggttgccg
    37921 ctgctggagc cgatgagtga aatcgccggc cggatggcga cgatcgtcgg cgcctattac
    37981 ctgagcaagg cccagggggg caagggggtg ctgctgggcg gggtgccggg ggtgttgccg
    38041 ggcaatgtgc tggtgatcgg cggcgggacg gcggggatca acgccggccg gatggcgatg
    38101 ggcctgggcg cggcggtgac gatcctggag gtcgacctgg tcaagatgcg gtttctggac
    38161 atcacgctgc agagctcggc gcatacgctg ttttcgaacg agcagaatct gatggacacg
    38221 ctgccgacgg tggatctgct gatcggcgcg gtgctgcggc cgggcgcgcg ggcgccccgc
    38281 ctgatccggc gcgagatgct cgacgtgatg cagccggggt cggtgttcgt cgacatcgcc
    38341 gtggaccagg gtggttgcgc cgaatcgacg cggccgacga cgcatgacga tccggtctac
    38401 gtggagcacg ggatcacgca ttactgcgtg gcgaacatgc cgggggcgta cgcccgcacg
    38461 gcgacgcaag cgctgaccaa cgcgacgatt ccgtacgcag cgaagatcgc caacctgggc
    38521 gtggagcggg cgctgcgcca gatggcggac ctgcgtcagg ggctgaacac ctggggcggc
    38581 ggcgtgattc atccggcggt ggccgaagcg accggtcacc cgtgtcaagc caatccattt
    38641 gatcatcccg acggagtacc ggccgcgcat tgagccctgg catcgaatcg cacccgcgcc
    38701 atcgccggat cctggagctg ctcgcccgcg acggcgaagt gagcgtgcaa cagttgagcg
    38761 cgctgttcgc ggtgacgccg atgacgatcc ggcgcgacct ggaggcgctg gagcgcgagg
    38821 ggcggctgac gcggacgcac gggggggccg tctattcgca gcgcgcggtg attgaattcg
    38881 ccttcctcga gcgcgggcgc gagcgccagg ccgagaagca ggccatcgcg cgcgaggcgg
    38941 cccggctggt cgaacctggc atgcgtctgg tgctcgatac cggcacgacc tcgctcgcgg
    39001 tggcccaccg gctcacgggg acgccgcgcc tgacggtgct gacaacgtcg ctggcgatcg
    39061 cctcggccct gcacgcgcac gaaaacatcg aattgatcct gctggggggg aacgtgcgac
    39121 gcagcagccc cgacctgacg ggcccgctca ccgaggagaa cctggcgcgg tttcgcgtgg
    39181 acatcgcgtt tgtcggatgt gacggcgccg acgcgacggg cgcctacacg accgacatgg
    39241 cggtggcgcg cgtgacccgg gcgatgatcg gctgcgcgcg gcgggtcgtg ctggtggccg
    39301 acagcagcaa gttcggacgg gaagctttcg tgcgttacgc ggcctgggag aacttccagc
    39361 aggtcatcac cgacggcggc ctgaccagcc gcgcgcggaa atggctcaag aaggcgcggg
    39421 tggaattgcg ctgcgcgagc tgaccatgag cgatggcggg gggcgggggg attctgttac
    39481 catgtggcgc gcgcgtcgcg cccgcgccga tgccggtcgc ccgccgaacg atcccgcgct
    39541 ggatcaattc atggtttcac gccactccaa aagaaagtcc ctggcgcggc tggcgttgta
    39601 cccactgctg gcggcgccgc ggctgttcgg cgccgggatc gggctggatc tggaaccgtt
    39661 gccggaaccg gccccgccgc cggcggtgac gctgcccgcg gaaacggaac cgggcgcgcc
    39721 cgaggcggcc gccccgcccg cgatcaatct ggatgaactg tatgaggcgg gtccggacac
    39781 gcccgaactg ggcgcggcgc ccgcggcgac gccgggtccg gtccgcccgc cgatctcact
    39841 cgacctgctg gaggaactgg cgctggccga tgccggctgg cggctgatcc gtgtcggcga
    39901 cgcgccgacc tcggtcgtga tccatggaag ctgggacgcc gtgcggtcgg gcgaactgac
    39961 gccggccgat ttccgcccgt tgagtccgcc ggacctggac gccggcctgg gcctggcgac
    40021 ggtctacggc ccgcggaccg ccggctggtt cgtcgccggt ggccgccgcc tgcacgtgaa
    40081 aagtaccgac gccagcatcg gtttccgcaa ctactcctac gagtcgctgg cgcggctggg
    40141 ctgggcggtc ggcgtcgccc ggggggtcca accggcgctg cgaatcgtcg ccgccggcca
    40201 gccggtctcg gcgatgaacc tggcggagtt cacgcggcgg gccgtggcgc gtgcgccggc
    40261 gaccgatgcc tggctgatcg ccggccaggt tcacctgccg cgggcggttg ggcgggcgct
    40321 gcggctggcg ccggtgggcg gccatgcaat gaacggggat gactcgccgg aaaacccggc
    40381 acgatccttt ttcgggccgg tgagcgagcc cgacatctgg gcgctgctgg tgatcgtggc
    40441 ggtgcgtccg gcgagcgggc agcctgcggc ggcggcgttg cgcgagaagg tttttggcgc
    40501 ccgcggcgag gcggatgacg gggatctgct gatccgcgcg cattacgggg tgttcctgcc
    40561 gccggcgccg gcgatcgatc cggatgcgcc gtttcagcgg atctgggacc agtcggtgcg
    40621 cagcccgcaa tttccgcgga ttgcatccgt ccgccagctg ctgcccgaca gcacgatcga
    40681 tgcggcgcat gtgtcggtct gggccgtggg tcaggtgctg ccgtatgacc tttatcagga
    40741 accgaagttg gtcgaggtga cggcgctgga cccgacgatc cagaccgagc agatgtgggc
    40801 gacgggggcg acgctggtca acgagccgat ctatgagcgg ccgcgactgc tgctgcgccg
    40861 cgaagtggcc gaacggctgg tgcggatcaa tgcccggctg aaggaacagg gctaccggtt
    40921 gaagctgtat gacggctatc gtccgctgtc ggtgacgcag cggctgtgga aactgcatcc
    40981 cgacccgcgg tacctggcgt cgccgacggt cggctcgcgg cacaaccgcg gcgcggccgt
    41041 cgacatcacg ctgaccgatc tggcgggcaa cgaactggag atgccctcaa aatacctgac
    41101 cttcgacatc acgtcgcacc ggacatggga agggatgacg ccgacggcgc gcgccaacat
    41161 gaacctgctg acccaggcga tgcggtcgga gggcttcacg acgatcaacg aggagtggtg
    41221 gcactacgac gcgccgggct ggcgggatta cccggtgctg gatgtgccgc tgtggcccga
    41281 tgagccggcg cggtgggttg ccgaggggaa ccatgatccg gcggccgcgg cggagatcgc
    41341 cgaaaaggcg agccgcggcg acacgtcggt atccgccccg gagaccgggc gggaggacgg
    41401 cgggagcgaa ccggccgatg ggagcgacgg tggcgaggac gcgcggcgga ggacgccgtg
    41461 ggcggcgaat tgacccgggg cggattgcct tgagttcact ccgtcttgac gggccgcgcg
    41521 gggggtggct actctttgga tgactgccaa cttgcggaaa gaacctcccg gtgagtgcgg
    41581 acacgcccca acccgaaatc acggtgctga tcgcggcgca caacgaggcc gaacggatcg
    41641 agggatgcct gcacgcggtg ctggaacagg actacccgat ggagcgggtg gaaatcgtgc
    41701 tggtggatga tcgcagcacc gacgacacgg cggcgcgggc acggcgactg gggattgagc
    41761 ggctgcgcgt gttgcgcatc gatgaggcgc cggccaatct gacggcgcgc caggcggcgc
    41821 tcgatcgcgg cctgatcgag gcgcgcggcg agatcgtgct gatcaccgac gctggggccc
    41881 gcgtcccgcg cgaatggatt cgcgaactga ccggccacat gggctatcgc gacgcggcgg
    41941 tcacggcccc ggtcgtgttc gccggcggcg gccatttttt cacctccatc cagacgctcg
    42001 acgccgtcct gacgctgacc tactaccgct gggcggcgcg caaccactgg cacaccggcg
    42061 tgctgggggc gaacctggcg ctgcggcgtg aggcctacct ggaagtgggg gggtttcccg
    42121 caatcggctt cgccccggat gagaacatcg cgctgtgcca ggcgctgcgc gcccggggct
    42181 ggtcgatccg ctacctggat tcgccgaccg tatgcaaccc ggcgtgcacg cggctgcccg
    42241 agctgatgct gcgctggcgg cgcaagacgc gcaatggccc ggccgggctg acgctggtca
    42301 cgctcgggct gatcctgagc aatctggcgc tgctgggcgc ggccctggtg ctcggcgggg
    42361 gctggtgggt gatcgtgctg ctgcgctacg cactgggggt tgtcgcgatc gcgggggtcg
    42421 tcatgcaata cggcgcgctg aacgttgcga tggcgatggt cctgttcgag ccgttgatga
    42481 cgctgctggg gacgtgggtc tatctgagca atctgctggt gccccgctgg cgctgggccg
    42541 gggtgtggta tgaccgtcgc ggaccgcttc ggcgcggctc gctgtcgcac ccggcagcct
    42601 gacggggggg cgctcgaatc gatccatgcg gctgttgctg atcatctatg agtatccacc
    42661 ggtgggcggc ggggcgggca agagcgcccg cggcctggcg cgcgcgctga cccgcatggg
    42721 tcacgaggcg acgatcctga cctcgcgctt cgaggggctg ccggccgagg aggtggtggc
    42781 gggggtgcgc gtgctgcgca tcccggtctt gcggcggcac ctgaactacg ccgcgccgtg
    42841 ggaggtgttt tccttcgcgg tggccgggat gatgcggatc ggctggctca aatggaaggt
    42901 gcggccgcag gtcgtgctgt cgtactttac gatcccgggc gggccgatcg cctggctcta
    42961 ccgctggcgc aacggcgtcc ccttcgtgag tctgctgcgg gggcaggatg tgccgggtta
    43021 ccccgacacg ccgcgctggc tgggctggct gatggcgccg gcggcgcgct tcatctggcg
    43081 gcgatcgctg ttcgtgacgg ccaacagcga gagcctggcc gagctggccc gccggacgat
    43141 gcccggcttg gaaatccctg tcgtggccaa cgcggtggac gtggaacgct accagccggc
    43201 gccgggcgag cggggcgggc cgccgctgcg catcctgtac ctgggacggc tgcgcgagtt
    43261 caaggggatt caggatctga tcgcggggtt tgcgcgcctg cgcgagtcgt tcagcggacc
    43321 cagcgagctg acgatcgccg gattcgggcc gcaccgggtg aatctggagt tgctgacctc
    43381 gcgcctgggg ttgaaggatt gcgtcagctt cacgggtcgg ctggaggaac cggaagccgt
    43441 ggcgcatctg cagcgggccg agatcgtggt caacccctcc tatggcgagg ggatgcccaa
    43501 tgcggtgctg gaggcgatgg cctgcggcgc ggcggtgatc ctgagcgaca tccccgccca
    43561 tcgggagctg gcgcgggagg gggaggaagc gctgttctgc gcgccgcgcg acgcggccag
    43621 cctggcggcc gcgctgcagc ggctggcgga ggatccggac ttgcggcgcc ggctcggcga
    43681 gcgggcccgc gagcggatgg tccgggactt ctcctatgag aaacgggccg agcagctcct
    43741 cgcgctgatc gcggcgcgca ccggcgggga gccgctcccg gacgagcgcg gcggttgaat
    43801 gttggcgccg gcctgtgaca ccttgaacgc ggcgcggcca gccctgcgac gccgtgcgct
    43861 cgggagacct catgcagctt gagcgaaccc ccatcgacgg tctgttgatc ctgcggccaa
    43921 aggtctttca ggatcaccgc gggttttttc tcgaaagcta taatgaaggg cgattccgcg
    43981 aactgggcat tgaactcccg tgggtgcagg acaaccacgc gcggtcggtc aaaaacacgg
    44041 tgcgcggtct gcattttcag cgcggtgcgg gtcaggacaa gctgctgcgc tgcgtccgcg
    44101 gccgcgtctg ggatgtcgcg gtggacctgc ggcccggctc gcggacgttc ggcgagtggt
    44161 tcggcatgga actgagcgag gacaatttcg cgatgctgtt gatcccggcc gggttcgcgc
    44221 acggctacgc cgtcctgagc gacgtcggcg aggtgatcta caagtgctcg cgcgtatacg
    44281 atcctgcgtt ggaagacggc gttcgctggg acgatccgcg gatcggcgtc cagtggcccg
    44341 ttgcggagcc gattctgtcc gagcgcgacc ggacggcgca gacgctcgat gaatacctgg
    44401 cccggcacgc gagcggggcc tgagccgcca tgatccccgc cgccgccatc tccgcccgtc
    44461 agccgtcggt gctgagctat ctgaatccgt ggcgcctggc gcgcgacctg ctcgcccact
    44521 gggagctgat ccggcaattc acggcgcgcg aggtgctcgg ccgttacaag ggctcgtatc
    44581 tcggcctgtt ctggtcggtg gcgacgccgc tgctgatgct gggggtctat acgttcgtgt
    44641 tcacggtgat cctgacgggg ggaaagtggg gcaatctggc gccgcgcgac gcgtcgcccg
    44701 cggcggaggc gcatcccgcc gattttctcc tcaacatgct gtgcggactg atcctgttca
    44761 atatcttcgc cgagacgatc tcgcgcgcgc cgacgctggt cctgagcaat atcaattacg
    44821 tcaagcgcgt cgtgtttccc ttgcatattc tgccgatcag tgtgctgctg ggcaatctga
    44881 tctatgggct gaccagcctc gtgctgctgc tggccgcgct gctctatttc acccatggcg
    44941 tcccgtggac gatcgtgctg tttccgctga tcctgatcgc gctgctgatg ctctgcactg
    45001 gcctggcctg gtttctggcc tcgctgggcg tctttttccg ggatattcag cacgccgtga
    45061 tcgttgtggt gcagcttctg ttcttcatga cgccgatctt ctatccggcc gaaatgatcc
    45121 cggagcggta tcgctgggtg ctgatgctga atccgctggc gatgctcgtc accgatgcgc
    45181 ggcgcgtgat gatcgagggg gtgctgcccg tctggagccg ctacgggatc gtgctggcca
    45241 tatcgttcgt ggtgatgctg gcgggctatg cgtggttcat gcggacgcgt cgctggttcg
    45301 cggacgtgat ttgaggaaag gcgggagtca ggagcgagat gagcgtcaag cgcatcgcca
    45361 ttgtctgcgg tacccggccc gaggcgatca agctggcgcc ggtctacctg gaactggcgc
    45421 ggcggcgcga ccgcttcgag ccgctgctgt gggcgacggc gcagcaccgc cagatgctcg
    45481 atcaggtgct ggaaaccttc ggcctgcggg ccgaccgcga cttcaacctg atgcggccgg
    45541 ggcagacgct gacgatgatc accacggccg tgctcgaggc gctcgagccg gcgctggcgg
    45601 ccgaccagcc tgatctgctg atggtgcagg gcgatacgac gacggcgttc gcgggggcgc
    45661 tggcggcgtt ttacgcccgc acggcggtag cgcacgtcga ggccgggctg cgcaccgaga
    45721 cgaaattcgc cccctggccc gaggagatga atcggcgcct cgcctcgcgg ctgaccgatt
    45781 tgcattttcc ccccaccgag cgggcgcggg ccaacctgct ggccgagaac gtccccgccg
    45841 accggatctg ggtgacgggc aatacggtga tcgacgcgct ggcgatcgtg gtggagcagg
    45901 tgcgggcgcg gcgaccggag ttcccgccgg attttccgct cgaccggctc gccaacggcc
    45961 gccgcatggt gctgatcacc ggccaccgcc gcgagaattt cggtcccgga ttcgaatcgc
    46021 tctgccgcgc catcgccgag ctggcccgcc gcaaccgcga catcgagttc atttatccgg
    46081 tgcatctcaa tcccaacgtg cgtgagccgg tcggccggat cctcaagggg atcgacaatg
    46141 tccacctgac cgagccgctg cagtatcagc cgttcgtctg ggcgatggac cgctgccact
    46201 tcctcctgtc cgattcgggc ggggtgcagg aggaggcgcc gcacctgggc aagccggtgc
    46261 tggtgatgcg cgagacgacc gaacgccccg aggcgctgga ggccgggacg tcgcggctgg
    46321 tggggaccga ctttgagacg atcctgcgcg aagcgacgcg cctgctggag gatgaggcgg
    46381 cctatctggc gatgagccgc gcgagcaatc cgttcggcga cggccgggcg gcggtgcgca
    46441 tcgccgaggt gctcgaacgc cacctggggg tgctggcacc gcgatgaccg gcaagcgcgc
    46501 cgagatcctg gtgatcggtc accgggggat gctcggccag gatctgatgg cgcggctggg
    46561 tgcgcgcgcg gcgggcgccg accgcgagga gtgcgacgtg acccggcgcg acgccgtgga
    46621 agcgctgctg gacgagctca agccggcgac gatcatcaac tgcgcggcct atacggcggt
    46681 ggatcaggcc gagagcgagc cggagctggc gcggtcgctc aacgccgatg ccgtcgagaa
    46741 cctgggcctg gcggcgcgcg cgcggcgcat ccatctgatc acgatcagca ccgactacgt
    46801 cttcagcggc gagggggagg ccccctggcc ggaggacgcg ccggcgtcgg ccttcgcgcc
    46861 gcgctcgatc tatggccaga ccaaactgga aggcgaacag cggctgcggc ggatcggcgg
    46921 cgagttctgc gtcgtgcgca cgcaatggct ctacggcgcc ggcgggcgca acttcgtcga
    46981 tacgatggcg gcgctgagcg ccgagaaacc ggtgctgcgc gtggtgaacg atcagctcgg
    47041 cgccccgacg tggacgcgcg acctggccga cgcgctgact ctgctggccg agcggcgcgc
    47101 gaccggcttc ttccacgtcg ccaacagcgg ttttgcctcg tggtacgacg tgacgcggca
    47161 catcgtgcag cggctggggc gcgcctgccg cgtggagccg tgcacgagcg aggagtttcc
    47221 gcgcccggcg cggcggccgc acaactcgcg gctggcgcag ggaaaattcg cggcgctggc
    47281 gggatcggcg ccgcgcccct ggccggcggc gctggatgcc tacctgagcg aacggcatgg
    47341 tcggaacacg gacgggaatc ctacattttc gtagcccctt gcccgtcgcc ccacgccgag
    47401 gtagtccctc ggccgaaggc tgacccatcc ttccgttgct gctgattcgg cgaataatcc
    47461 taaaaaatca gggctttcag cgcagcgcgg gccggcccgc tgaggaggcg cgcgccatgg
    47521 cacagccttt gcagtagggg gcccgccaag agtgcatcgg gagagcgact ctccgggcgc
    47581 ctcgctgaaa gagtcctgta tagccggtgg agcgggcgga aaaacgcgcg gcccacccga
    47641 cggggaaatc atatccacag ccgcggggtc gagtgcttcg gcaaaatgca gccgcggcgg
    47701 aagggagaag tgcatcctgg atcgacggaa ccacgggacc gctaatgcta atggaaatta
    47761 ccgaatacat tcaggatcac aaatggcacg aaagaactgg aattgattac gacaacgagg
    47821 gacgagcgac gagcgacgta ttctgagtga gggacgacat tcctggacga aaggggctga
    47881 gacatgaagt tgagcaaggt cgaatgcatc atcaagccgt tcaaactgga ggaggtcaag
    47941 gaggcgctga cgcatttcgg catcagcggc ctgacggttt acgaggtgcg cgggttcggc
    48001 cggcagcgcg ggcacaagga aatctatcgc ggaacggaat acaaggtgga tttcatcccg
    48061 aagctgaaga tcgaactgct ggtgacggcc gatcaggtcg acgacgtggt cgacgtggtc
    48121 gtcaatgcgg cgaagacggg cgccatcggc gacggcaagg tctacgtcac gccgatcgag
    48181 tcgctggtgc gcatccgtac cggcgaaaag gatatcgatg cgctgtagct cgccgccggc
    48241 gccgcctaca cgttgaattc caatgataaa aggaataaag ccaatgaaat caaacggaat
    48301 gaagggactg acgggaagac tgttccggtc gcgcgcgctg ctcggggccg cggcgctgct
    48361 gctgatggcg ggagcggcct gggcgcagga agaggccggg ccgtcggcgg agcagttcag
    48421 tctcaacaat ctgctgctgt tgatctccgg agcgctcgtc ttctggatgc acgccggttt
    48481 cgcctgcgtc gagtcgggtc tgacgcaggc caagaatacc gtcaacattc tctccaagaa
    48541 cgtcatcatc gtctgcctgg gcggcgtcag ctatttcatc atcggcttcg gcctgatgta
    48601 tccgggcgag ttcaatcact ggatcggctt cggcggatcg gggttcggtc ccgtctcgcc
    48661 cgaagattac ggtgattaca cgatcttcac ggatttcttt ttccaggcgg tgttcgcggc
    48721 gacggccgcc acgatcatct ccggcgccgt cgccgagcgg atcaagttcg cctcgttcat
    48781 cgtcgtggcg ctcctcttcg tgacgttcat ctatcccatc gccggctcgt gggtctgggg
    48841 cggcggctgg ctggcggacc tcggcttcaa ggatttcgcc ggctccaccg tggtgcattc
    48901 gatcggcggc tgggcggcgc tcaccggcgc cgtaatcctc ggcccgcgcc gcggcaaata
    48961 caccgccgac ggcatccgtc cgatcgtcgg ccactcgatg ccgctggcca cgatcggcgt
    49021 cttcattctg ttccttggct ggttcgggtt taacggcggc tcggtcctgg cgatggattc
    49081 tggggcggtc tccatggttt atgtcacgac gtttctggcc ggttgcgccg gcgggatcgg
    49141 cgcaatgctc gcctcgtgga tcgtcctgcg caagccggat atcacgatga tcctcaacgg
    49201 catcctggcc ggcctggtcg gcatcaccgc cggcgccgac acgatcgcgc cctggccctc
    49261 gctcgcggtc ggcgcgatcg ccggcgtgat cgttgtcttc tcggtggtca cgctcgatcg
    49321 gatcaaggtc gacgacccgg tcggcgccat ctccgtgcat ctggtctgcg gcatctgggg
    49381 cacgctcgcc gtcggcatct tctcgttcaa ccccgaccat ggcttcctca cgcagctgat
    49441 cggcatcgtc gccatcggcg tgttcgtgct ggtcacgagc acgctggcct ggctggcgct
    49501 gaagcactcg ccgctcggcc tgcgggtcag cgaagaggaa gaggtcaacg gcctggacac
    49561 ctacgagcat ggcctcgaag cctaccccga gttcaagtcc accacaggca tcctctgagg
    49621 cttcactccc ggcctcagcg atgcctctcc ccccgggtta tgcgtgactc ccgtaagcgc
    49681 atgacccgct ctccccccgg aagccttcgc ggcttccacc atcgggtggc ccgggttacc
    49741 tctcctggcc cgggccaccc cctccccccc tccaaatcaa gttcgtccct cgtcctcgtg
    49801 atcggcccct cccgtgagat gtgagatcgg agaagggaca tcaaggacgc aaaggacagc
    49861 agggacgaat cggaagcccc ccaatgcggt tccaatgtgg accgtttggc gtccctcctc
    49921 gtccgcgcgt tcagccttcc tcttcgatgc cgtcgggaag cgtgacggga atggcgggat
    49981 tctccggccg cgcatggcga ctgttccaca atgcggtaac cagcgcgggt tcctcgggat
    50041 tcgcggtcag ggcaacggcg agcgcctgcg cggcaagcgg aatctggccc tgcgcgtgcg
    50101 tcaactcgta gcgccggacc caccagcgcc gctcgccggg cgccagcgcc aggccgtcga
    50161 cctgcatgtt gtaggccagc gacgtgcgat tctgggccag cgcgctgttg tagagccagt
    50221 cgatcagctc gatcgagttg ggcatgcgcg ccagcgcggc ctgcacggtc tcgagcgcct
    50281 cctcctcgcg ccggcaaagg agttgaaacc gcgccagttc aagtgggtac tgcgtctcgt
    50341 atggatccag cgcgatcgcc cggcggtagg accggacgac attgaggtag ttggtctcgc
    50401 tccagccggg cgactcctcc atcgtcttgc ggtagctgac ggccagcgcc cgcgggattt
    50461 ccggaatgaa cgggttggcg cgctgagccg ccagcagccg gccacgcatg acggcgctgg
    50521 gcaggtcggc gcccgcggtc tgccgcagga tcacctggcc atgcgtcggc ttgaacagca
    50581 ggagcgagag gatcacgccg atgaagccca ccagcgccag cgcgaggcgc cggttcagcc
    50641 cggcggccga atgcgtccgc atgcgggtgg gcacatcggc cagggccagc gcgctccagc
    50701 cggcgatcac cagcagcacg acggctgaac gcgccccgcc ggtcaactgc aagccggcga
    50761 caaacagcgc caccggcgcc gccacttccg ccatccgacc ggtgggaccc ggcggccggg
    50821 ccacgcgcgg ctcgcgatgc aggattagga atgcgatcca gaccagcagc agcgtcaggc
    50881 cgatcagccc gccgatcgcc agcaggccga acagcccgcc ccagtcgggg atcatgacgg
    50941 ggttggggac ggcgagcacc tcgcccagcc ggccggcggt gccatggccc cagccgaaca
    51001 gcaccgattg catccagagc gcctcctgcg cgcggatgcc ggcctcgagc atcagcgcgc
    51061 ggtcgacagg caggcgctgc tggctgatga aatggttcca gccgaccgag ccgccccaca
    51121 ggaccatggc caggtgcaag ccgatgcgga tcggccgggt gatgatgcgt aacggatcgg
    51181 gggtggaatg aaccgcgtcg atgccgtgcc cggccggccg ccgggaaatt gcgcgcagtg
    51241 cgctgatcac cgccgccgcg cccaacccca gcacgaccga ccagcgcgtg gccacgaccc
    51301 atggcgaggc ggtgggcagc cgctggtgca tcgaccgccc gaccagccag gcgatggcga
    51361 gcgcccccag ccagaaccac caggcctgcc acggccggag ccgcgccaga aaccacaggc
    51421 cgaccgccac ggtggccatc acggccagtt cgagcgcctc gccgatcccc atgcccggca
    51481 gcggaccgcg gatcgagagg atcgcccgga cgagcgcggg cagcaggatg agcagcagcc
    51541 atgccaggtt gcgcagatcg aaatcgggcg cgccgacgcg ccagatcatc agcgccacgc
    51601 cgacgacggt gagcggcagc ggcagccagc gctcccacat cgcggccggg aaatcgagcg
    51661 tcatcagcgc aaagatcagc gccagcagga tcatcgcgcc gggcagatag cgcagaaact
    51721 ggccgcccag ccgccgccag tcaacccggc gcaactggcg catcagcggc gccgtggagg
    51781 gcaggcgcag gcggcccccg tcgcgacgac gcggaagcca gctcaggatc ttggacaggg
    51841 gactcattga aatccagcga ccgctccacc acggactgcc gcggccgaag catgcgccgc
    51901 ccggcccacg cccgacttta ccccacgccg ggcagggcgg gcatcaggaa accggcgggc
    51961 ggcggagggc cgtccgcgcc ggcggcgcga cctcaggctc tgactttacg cggcgaacgg
    52021 gccgagcgct gctccgcgcc ggacccattg aggaacgggg ggatttcctc gcagccgatg
    52081 atctggtcaa tcgtctcgcg atgacggatc aggtaggcct tgtcctggcg cacgaggacc
    52141 tcggccggtc gcggccgcga attgtagttc gaggccatcg tcatgccgta ggccccggcg
    52201 tcgcacacca gcagcagatc gtcgggcttg agcggcggaa aggggcgatc cttgccgagg
    52261 aagtcgcccg tctcgcagat cggcccgacg acgtcgtaga cgcggcgccg gccgcgcccg
    52321 gggcgaccgt cgagcagaat ccggtgatac gccgagtaga gcgagggccg cagcaggtcg
    52381 ttcatcgcgc catcgacgat cgcgaagcac ttcgtctgcg cttctttaat atacaggaca
    52441 cgggtcagca ggaagccggc ggggccgacg atcgaacggc ccggctcgaa aatcacctgc
    52501 gcccgcagct cgcgcagcat cggcatcatc cgccgcgcca gctcctccag gtcgatcggc
    52561 tgggtcgact cctcgtagcc aatcccgaac ccgcctccca gattaaccag gtcgatcgac
    52621 aggccgtcgc ggcgcaactg atgggtcagt tgcacgaccc gctccagcgc cttgacgaac
    52681 ggctcggtct gcgtgatctg cgagccgatg tgcgcatgta cgccgatcag ccgcagatgc
    52741 ggcatccggg ccagccgccg gcagaggcgg cgcacgtcgt gatacgggat cccgaacttg
    52801 gtctccttgc ggccggtgct gatgtaatga tgcgtatcgg ccgcgacatc gggattgacc
    52861 cggaccgcca cgggggcctt gcgcccgaag ccgctggcga cttcattgat cgcctcggcc
    52921 tcggccggcg actccacatt gaaggcgaga atccggacgt tgagcgcggc ggcgatctcc
    52981 tcgcgcgtct tgccgacgcc ggcatagatg atccgggcgg ggtcggcccc cgcgcgcagc
    53041 gcacgctcca gttccccccc cgaaacgatg tccaggccgg ccccgcgctc cagcagaatc
    53101 cgcagaatcg tcccgttggt gttggacttg gtcgaaaagg cgatcagcgg atcgatctcg
    53161 gcgaacgcct gccggatgcg gtcgtagttc gccaccagcg tggagtggct gtaaacatag
    53221 agcggggtgc cgaatttgcg cgcgagcgtg gcgacgggga cgccgtccag cttcagggcc
    53281 tgcgagcgcg ggtctcgttc gggggcggtc attggggggc gattgctcct tcgtggctgg
    53341 atgcggcggg cctgcacgct cagggccatc gtgggtccgc gccgcgcgtg ctggcgggtt
    53401 cccggcgcgg gccgggcgct ccgccgcgga acggaccgaa ccgtgcctac agcaaaccga
    53461 cctggcgcat cgcctcgcgc agtttggcgc ggctggcttc ggaaatctcg cacagcggcg
    53521 cgcgcagctc cggcccgatc aggcccatca tttccagcgc cgccttgacc ggaatcgggc
    53581 tggtttcgat gaacagcgtc ttcatcagcc ggaaatatcg gtggtgcagc gtgcgggcct
    53641 gatcccagcg gtcggccagc gcatgggcca cgagctcggc catcggcccc ggcgcgacgt
    53701 tggccagcac cgagatcact ccgcgcgcgc cgacggcgat ctgggccagc gtcgccgaat
    53761 cgtcgcccga caggacggtc aggtcgcacg ccccgcacac ctcgctcgtc cagtccaggc
    53821 tggccgtggc gtccttgacc gcgacgatgt tcttgagcgg cgccaggcgc gccaccgttt
    53881 ccggcgcgat cgacaccccc gtgcggccgg gacagttata gacaatcagc gggagatcca
    53941 ccgtctcggc gattttgcga tagtggcggt agagcccttc ctgcgtcggc ttgttgtaat
    54001 acggcgtgat cagcagggcg gcatcggcgc cgtcggcgcg ggccgattcg gtcagccgca
    54061 gcgcctcgcg cgtcgaattg gaaccggtgc cggcgatcac cggcacgcgg ccggccacga
    54121 tctcaaccgt cagccgcacg agctgctcgt gctcctcgtg gctcagcgtg gccgattccc
    54181 cggtcgtgcc gcacgggacg atgccgctcg tgccgttctc gatctggaat tcgaccagcc
    54241 ggcggaaggc ggcttcatcg accttcccgt cgcggaacgg cgtggcaatg gcaacgatgg
    54301 aaccttgcag ttgcagcgag gcggagggct gcatgaaagc atccttttga tcggggcgga
    54361 acgacccgcg cgcggccggc tcaggtcgtc tccggcatgc atccgtggca ttgaggccgc
    54421 cgccgccacc cccggcggcc cggccaaagc aacattaagc ggaaggcacg cggggaagtc
    54481 aagcggcagc gcccgcgaca acgcgagggc agcgcagacg ccagtactat gagatccgtg
    54541 aaagggggga attcgaccgc gcctatcttg acgtctcttg cgctccctcc tgctcccgcg
    54601 cgtcgccctc cagggcggcg atccgctcgt ccagcttctc gatcaggcgc agggcgacga
    54661 cgtgggcgcg gcgcatttga tgcagtcgca ggatcagaaa ctcaaacccc aggacgagcg
    54721 acagcagcgt caggatgaac cagaagacga tgaagtggcc catcgggtcc tcgcgcaggc
    54781 cggcatactg caccagcgag gcgagcaggc cgagcgccgc gccgaactgc agcaccccga
    54841 cgcccgcctc ccaccagcgc ggcatgagct ggtcgcgaag ccgaaccgaa tcgcggatgt
    54901 cgcgcagccg gccggcgacg gtgaaatcac ggatcagttc ttccttgtct cgcggatagg
    54961 ggggcggaac gtcgcgacgc gccatggccg ttcacctcca gggtcgggaa gccgttcggg
    55021 cgattcagga tagcaaagcc ggccggcggg gggaacggcg gggccgcgcg gcgcgtgcga
    55081 aggccgggcg gcgtcatgag ggcgcggggg gctggccggc ggcggtggaa acaattgcga
    55141 tgaatgccgt ttagctgtga acatttttgc tgccctcccc tcgccctcgc ggtatcatgc
    55201 cctgcccgtc gtcattggcc acgcatctcc gttccgacac ccctccgtgc ccatggcgag
    55261 tcaacccgtc aagcctgccc attccaatcc gctgctggcc gccgcgcgcc tgctggccct
    55321 ggtcggcctg acgttcttcc tctatccgtt ttactacctg cattaccggc tgacgccccg
    55381 ggatcggcgc tacccgatct cggctcgctg ggtccatgcc tgggcggtga tggcgtgcgc
    55441 cgtcttcggg atccgcgtgc ggcgccacgg cccgccgccg ccgccggggg cgctgctggc
    55501 agcgaatcat gtctcctatg ccgacatcct cgcgctctcc tcggcgatcc cgtgtctgtt
    55561 cacgccgaag tcggagatca agagctggcc gctggcgggg ttcttcgtgc gcacgaccgg
    55621 ccacccgttc gtcacgcggg cgcgcggccg cgatatcatc aagacgacgc gctcgatcga
    55681 gcggcgcctg cgcgccggca cccacgtcgt cgtctttctg gaggggacca cgaccggcgg
    55741 cgaccgtctg ctgccgttca agccggcgct gctgggcgcc gccgagagca ttcaggcccc
    55801 cgtcgtcccg gttgcgctgc gctggtccag ccgccacccg cgcgtcgcgg ttcagcgcga
    55861 catcgcctat tggggccgcc acgtgatcgg cccgcatgtg tggcggctgg tcggcctgcg
    55921 cgggacgcag gtcgacatca cgttccacga gccgatgcgg ctggacgccg gcgagcgccg
    55981 ccggctgctg gccgatcggc tgcggcggcg gatcgccgcg acgctcgacc tgccgctggc
    56041 actccccgag acgaatcccc cccagcccgc gagcgaaggg aaagaaacgg acgaacgcgc
    56101 gggagagaca tgagaaaaac gaggatgaaa gacgagcgac gatctcggac cggaccgtcc
    56161 cggtct
//
LOCUS       DAOPCO010000004        46333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_918_length_46333_cov_4.891849, whole genome shotgun sequence.
ACCESSION   DAOPCO010000004 DAOPCO010000000
VERSION     DAOPCO010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 46333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46333
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_918_length_46333_cov_4.891849"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1154)
                     /locus_tag="PLS90_00730"
     CDS             complement(69..1154)
                     /locus_tag="PLS90_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00957.1"
                     /translation="MPSAAVALSHRERVRAVYLGQPVDRIPLLYECPMDVTATKLLAP
                     PPTGDEVADEFAFAECFDNSAIGVGIKLACETLSQDEGHRVYRYETGLVVREQYRPVF
                     NRHVLKVPIESPGDALQFQFPEVHAPGRYDAEELRRRIAGYHAAGYFVEGWCIGGWGS
                     VYGYLARFDDALRWMLTEPEAAHALFDMLTRFSVESARVLLDCGVDAIFVGSDLGSGR
                     GLIFAPPLFDEYVSPWLKAVADLAHERGAWVHLHSHGHIQDLMDGIVAAGVDMINPVG
                     PSDYNDLALFKARWGDRIVLHGGISTRMQFMSEEEIRSHVREVMAVGRRGGRFIPRTE
                     SGIPPMRREQVRAYLDTLSEERAKGYA"
     gene            complement(1199..1696)
                     /locus_tag="PLS90_00735"
     CDS             complement(1199..1696)
                     /locus_tag="PLS90_00735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPK00958.1"
                     /translation="MTKPPSHPSPSAATLQVQAQYDTDCQYCRLMRDGTGPLERWLMP
                     LIETDTSIIYLHRDQFWPGRCVVALREHATELFQLDPAVRGRHVEEMIQTARAIDAAF
                     HPDKLNYECLGNLVEHIHWHLIPRYTWDFLWRRTPWDEPHEPRLLSAAEYAATADAIR
                     RQLAL"
     gene            complement(1761..2843)
                     /locus_tag="PLS90_00740"
     CDS             complement(1761..2843)
                     /locus_tag="PLS90_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00959.1"
                     /translation="MMIPVARVGDSTRIVEQALSFEPPERMPVFETIWPEWAAKWRAE
                     RGLDQSADVLDYFPLDLAVCAALEAPCVTHRRVLEDDGDAVVADDGWGRRTLTRRSGF
                     FCHVIDRDLKEKSDLDRLTFDPVNLDVRFETFDREVAGARAAGKPVFVKIGGLYNRCA
                     MLRGEAEFLMDLVSDPEFARALIERLAEHELQLGLEVLRRADAYAQGVWIFDDMCNQR
                     SPMFSPAIFESMFLPVYQSIIGRLKAVGARRVFLHSDGNLGPLLDLVVEAGFNGINPV
                     ERRTGLIVEELLPRYFGRLSFIGGVCNSVILPRGTEREIRRHVETLIDAGRLGGLVMG
                     THSIAPDIPVANYELYRSIVKAYRRH"
     gene            3191..5113
                     /locus_tag="PLS90_00745"
     CDS             3191..5113
                     /locus_tag="PLS90_00745"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HPK00960.1"
                     /translation="MLKTVCHSLLIPFLCIQLALPVWGAQWVWQNPLPQGHTLSSVQL
                     LDDSHGWAAGRDGVLLHTLDGGATWNVILTDGDLAFEDFVFLDSSNGWAITGIPYRLL
                     RTTDGGRSWTTVPTVDNPPPLDDLHFHDGQHGWIASETQVLRTTDAGLTWHAHETHAS
                     GTLAAIAFPDPLNGWAIANHFPNGASILHTTDGGLSWSPQTVAVSLDSTRLSALHFVD
                     AMHGWAVGLGGQILHTANGGADWSPQSSGLGIPLSDVFFIDSMVGWIVTSNWTLEHGY
                     QVLHTTNGGADWSPQATGAEQATNLLALHFANATDGLLVGESGILFNTINGGADWVAR
                     SSGPNDDFLDVDFSDSQSGIAVGTGNYLYLTENGGNSWQNVPIPPEDSKEIARLTHVW
                     KLLSNYFAGLVGATKKVIKVEDKNKGKLEAGDLTFIYLDPGVAGDIEALYGVDDLTWW
                     IATNAGEIRRTTNGGESWDPQASGVERSLNGFHFHDGLIGWVVGEQGTILKTTDGGDN
                     WFPQPSGTASNLNAVHFFDTKRGFAVGDGGRYLTTDNGGDKWEIGSLSVFHNLHDIAF
                     ADALHGWIVGSSTRALYTDDGGQTWTSQPTGASGLIWAVDAPDPDHAWIVGAGGAILH
                     YDGATDSSAAHWQLLR"
     gene            complement(5193..5762)
                     /locus_tag="PLS90_00750"
     CDS             complement(5193..5762)
                     /locus_tag="PLS90_00750"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK00961.1"
                     /translation="MPMPPTLPLIEWPKVFASGESYDQWITNKEYAENCKKMDEDRRG
                     LRLAAPVEGALKALPKTVHVLAIAEAWCGDVVRHVPVLQRMAEATDRVQVRYISRQQH
                     PDVFARFLTNGGEAIPKFVFLSQDFVECGNWGPMPWACRLWIARGKAAGDGAKARERV
                     GALYAADPNRTEVARELLELMEIAATEQV"
     gene            complement(5853..6650)
                     /locus_tag="PLS90_00755"
     CDS             complement(5853..6650)
                     /locus_tag="PLS90_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK00962.1"
                     /translation="MQLRIYHSFWGMTGSTQSKIEAAAMAGYDGIESALAPDLPIDEY
                     RGVLSDHKMDFIAAMSAVDGDELARQVESLLPLKPTRMRLHAGRDCMSREEGKRYFAR
                     VLALQEEIGMPILIETHRGRMFYSPWDALYYMKEFPALRVNADFSHWVNVCERLPDDQ
                     EEAMALACERSLHIHARVGYEEGPQVPDPAAPEAARYLEWHLKQWDRIRKHHEERGEE
                     LLTVTPEFGPPPYLHTLPYTNVPVADLWSVCLWMANQLRERWKDIAG"
     gene            complement(6890..7525)
                     /locus_tag="PLS90_00760"
     CDS             complement(6890..7525)
                     /locus_tag="PLS90_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPK00963.1"
                     /translation="MPVFRSGKGQAPAWCELEQFDIVRLQPGQTHSFTREGRKEKLIA
                     AKGKCKVRIGREEKMLEHGTIVDLTEPGLNFEVTEVAEPVILTRMAGHWGDQVGGSGL
                     FSVVNTDKTDNPGGPVSYEKTTTFDAHYHDCDEYWILLEGRGEAVSEGKHYQVGPGDC
                     VATGRGFLHDFPKVEEPVLAVYFETTIEGEKRLGHLWPHTHGTPRPVAERV"
     gene            complement(7646..9721)
                     /locus_tag="PLS90_00765"
     CDS             complement(7646..9721)
                     /locus_tag="PLS90_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPK00964.1"
                     /translation="MSPVSTADLPIVIYAVSFWIWFFVWAPQRAWVTGLLHDDLPVWL
                     RQQSFKLLRLLMLPVFIYFAWGLTDYSQTLQGVDWAELLNQTLQGTYVGLFAGAAVLG
                     VVLWGFPLLIARTRVRKGIEPGEWSTAALTFYRWWALGILLVIGTGFTEDIAELEGLI
                     AVPLDAATATAYALGVSLLYFLGWFLPSVWLARLVEAGEPRAVDMQPKVRWLGLLGLL
                     MYCIQPRSKSPAVGRKGEGHKLCPSCMRPIDDIGRYESLQFENCPHCGEVIPPVFSIR
                     DYIVHYAHMVLEAQEFKAGKRKAAKKAEKQESDMVQRMLRAILTMGVRERGTDLHLIS
                     EGGQFLVRCRTDGILWTMVELPEELLRLVVSTIKVQCNLDIAERRKPQDGSFKTMADD
                     RKLDVRVNTSPAGDGETASMRLLYRHQVLGSLESLGMSKHAHGLLVDAIQRPHGMILV
                     TGPTGSGKSTTLYNSLGTIADGRRNIITLEDPIEFHIEGLTQMQVNAAKNFDFATGLR
                     SILRQDPDVIMVGEIRDGETAKMTVDAAMTGHLVFSTLHTIDTTTTIGRLLDLGVDPH
                     RHAEALLMVIAQRLVRRICHTCEEEIEIDADYFAKLGIPNAPAKIKARRGRGCERCHE
                     SGFYEREGIYEVLRPDGTIRQLIGNRAPSYEIRRAARALGMRTLLEDGLVKVVMGRTT
                     VEEVLRVTS"
     gene            9973..10914
                     /locus_tag="PLS90_00770"
     CDS             9973..10914
                     /locus_tag="PLS90_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00965.1"
                     /translation="MLPRILTPLGALLIVMLTGCARQQIIFVNAQHNRLGEPRLTVHQ
                     PGHYRVSVRRMANAPPWQDLVLRNDGRDPVDEIRLWGLGGADLLEVARPAGAEPPSPD
                     PLAEPAPRSVLKPFRLMPGERFVCGEREIDGDMHLQGAFLWAMGGDNTRILSAADTLR
                     NARSGRTTPIVLDPSSGEAYVQWTFSSPLPHLGGMLSWEVDPPGARPEVWISVDRGVW
                     GRLPNPRGGHQWAQPVDLTSSIEGYHEFSLRLLFAPDSVGVESEDGTITLRKIYLERP
                     FGGRGELREWRPGLNELEFAFRAHGNAQLTVELLNAP"
     gene            10937..12289
                     /gene="aroA"
                     /locus_tag="PLS90_00775"
     CDS             10937..12289
                     /gene="aroA"
                     /locus_tag="PLS90_00775"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPK00966.1"
                     /translation="MLPSRYTAVIHPSGPLGGYVQAPPSKNYTTRLLLGAALAEGRSL
                     IRRPATNDDARALVACLRALGAQIEETGPDLTVRGFASRPRNPGRINPGNAGAVLRLL
                     LAVACLVEGEVEFVTDHTDSLGRRPNEDLLAALRQLGADAAGQGSDGRLPIRIAGGPQ
                     RVRGGAVKVSGARSSQFLSALLFLAPHLTGDTTIEVTGADATAPPVLVSRPLIDQTLD
                     AMRRFGLRCDPDPSGFRFLVPGGQVGRAGDHAVNGDWPSAAALMAAVAVAGGMSTIHG
                     LQDDAQGERRAERLLAAMGCTFVRPEEHALVIHSKGNLQAADLDGDLATDAVLALIGA
                     ACLADGTSRFSNIGNLRLKECDRIREPLEELARLGVRARHGEDWIEIDGNPDGYDGGV
                     DVDSRGDHRVAQMLAIVGLRCRNGLRITRAESVSKSYPDFFADLQRMGVKLDLVPASD
                     "
     gene            12393..13520
                     /locus_tag="PLS90_00780"
     CDS             12393..13520
                     /locus_tag="PLS90_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00967.1"
                     /translation="MSDHPAGSLGAPPIGAGGLQSGWPAYAIPAALFLSGAAIFFLLR
                     PFGVPGGDSELLTSPQSMALYWRDSLVYFMREPLAHQIALQFHLAAGDLALGMALSGA
                     FCGGLFLVLLWEFCRSPIVWAAVLGSCLTWVFVGHLELYAPVAVTLLLYFMLLVRALQ
                     PGSGVTPFHAALALALAYLTHKLALFFAPALLCLAFERVAGRWRIRPWPAGAWERALV
                     AIIAMCLIDMLPALADMAGVHHILYITFNEHFLDMITPLTPSMARRLEARSETGMFFL
                     FTFGTPLHLAHFFGFILASAPLGLVLLARHWRRIRSAAALPLIVAAVIGILWILLWHP
                     RCGWRDWDLFSMGTLPLNLLGGGAAAGWFDADAANPPHPAH"
     gene            complement(13569..13811)
                     /locus_tag="PLS90_00785"
     CDS             complement(13569..13811)
                     /locus_tag="PLS90_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00968.1"
                     /translation="MSQPEEFQITIEPDGRIILDGRGLHETSYRRILELLAATVGPVQ
                     ELQRAPGEPPATALHRPAVKSEAALDTDELRLERGD"
     gene            complement(13909..15075)
                     /locus_tag="PLS90_00790"
     CDS             complement(13909..15075)
                     /locus_tag="PLS90_00790"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00969.1"
                     /translation="MRARCKERSLLFGCAAIGLSLLFGLLSVEIGFRIATGDRYRDLT
                     LNYFPPGSVIYDEVLGWNYRPGYAATWHYFEYAFDVRIDERGLRVDDDPPAPATAPRI
                     LFLGDSFTFGVGVDAGQTFVEQTEARLRAAGLVTECLNAGRVGGGTRAARQRLEQLID
                     ATSPTLVIHAFFPENDLADDWLGAAPDAARFTNHGGEPYVNGEPLAAARTTPTGPLFR
                     NSAFYRWLRMRIPGSINLGENPYQGMWFDERLIVPPDWVPAAEALTRAEWAAMQARCE
                     ARGAAFAVVLIPSDYDVDDRLWGRIVKRYGVARGHREEGYDRELPFAWAAQAGRDLGI
                     PALNLRPLARAAHARRGEPLYFQHDRHFTAAGHGLAAEAIAGFIQDAGLLPGGA"
     gene            complement(15162..17099)
                     /gene="ftsH"
                     /locus_tag="PLS90_00795"
     CDS             complement(15162..17099)
                     /gene="ftsH"
                     /locus_tag="PLS90_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPK00970.1"
                     /translation="MKGLSSVTLWIVIFMVFVLLFMHYNKKVTPTDLSLTQWIKQAEA
                     GNIIKAVDEGNVILSEIRDPNNSDAAPITYKTRYLAGQEGEVLQKALELNVPYDTDPS
                     NPYISQILISILLPILVIFILWMLLMRQIQGGGNKAMSFGKSKAKLVKEGEIKVTFKD
                     VAGVDEARDELGEIVEYLKDPKKFSRLGGRIPKGVLLVGPPGTGKTLLARAIAGEAGV
                     PFFSISGSDFVEMFVGVGASRVRDLFEQAKKAKPCLIFIDEIDAVGRSRFAGIGGGHD
                     EREQTLNQLLVEMDGFQENEGVILIAATNRPDVLDPALQRPGRFDRQVIVDLPDIKGR
                     EKILQVHARKVKMEVGVDLTRIAKGTPGFSGADLANLINEAALLAARRNKETVGYLEF
                     EEAKDRVMMGPERRSMVITDREKLVTAYHEAGHAIIAHFTPGTDPVHKITIVPRGRAL
                     GVTWILPTEDRRTMSRQQLMAQLRHLMGGRAAEIIQFGDYTTGASNDIERATGLAQRM
                     VCQFGMSDRLGPRAFGDHDGQVFLGRELGRDRNYSEETATIIDEEIKKLIDDAHDFAM
                     DLLREKSELLDRVSEALIERETLDETEFRMLVEGKTLPPMERATPPPPPPAMPKAAAE
                     DREREKRPHHPLAGPEAQPSV"
     gene            complement(17096..>17313)
                     /locus_tag="PLS90_00800"
     CDS             complement(17096..>17313)
                     /locus_tag="PLS90_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00971.1"
                     /translation="RGTVMLIQKAAEAALMPVCSVANRGFSCWPVFGSSASMRLDLAA
                     AETDAWLFIPVLLVESRSPISLSNRMQ"
     assembly_gap    17314..17413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17663..18499
                     /locus_tag="PLS90_00805"
     CDS             17663..18499
                     /locus_tag="PLS90_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPK00972.1"
                     /translation="MTSSSPRIVMACLSAMALISSVAGCASGGASGERAQFQSAAPAS
                     PVQPVPAAAAGKASPAAPAPLDAMTSATTGDQTITTTPLVLLYVAAMQEDPAFLDQVL
                     KSGIDINYVDPAFGMTAFHFAVGEGQFEVAQRLLDHGADINSRSVPSHATPLIDAVNL
                     NQTETIRWLLARGADPNVRDGTVGVTPMLSAAMDNQVDVLQLLLEHGADPNMMNGQGV
                     PPLHLAVSMGHLESVELLLAHGAEPNLPTGEAGVKPLTVALRHPERPEIIRLLKEAGA
                     TE"
     gene            complement(18462..19820)
                     /locus_tag="PLS90_00810"
     CDS             complement(18462..19820)
                     /locus_tag="PLS90_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00973.1"
                     /translation="MANTGGSQLHLPDNLSYECIRCGRSCGEFWEIPVSAEKAEEIRA
                     RPVAALAAARDPNNPVVESPWTPGRHVMRMTDGTCTLNTRAGLCALHAAFGETAKPNI
                     CRSFPFRFLETPRGDYVGLSFACTAVLNRLGPAVADQGDALRDLQRWTFSRHRIEEPP
                     GLTNLIPLSWEQYEAIERDLADLLDPALGPIDVRLVAQAAYMQLLVRFLREAREQTGQ
                     MTAGSEGQDKLLPAFRRRMRGEAGEPWAIPLRLARRRGASPLLRRMFLGFAHALRNTY
                     GERRGRLRSYVMVMNTYFRHAAGRGAVQLPTLPAATDWRRLREIRFDPRRPELDELLT
                     RYFRHRLFRKDLLQAETLHFGHCLQLMHWGLIHWYSACFANVAGAAEVDYEHLTEGLR
                     NVEKYYVYHSTFDRLFATYPLLRNFLDWIFIHPLYAYSMGYTEERAVGATPSPPPLSG
                     GG"
     gene            20132..21160
                     /locus_tag="PLS90_00815"
     CDS             20132..21160
                     /locus_tag="PLS90_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00974.1"
                     /translation="MAKQPAWHQALAAARGGDLDPARRLHAELPDDPLNDYFFGRCLA
                     EAGVELDRASELLRRAVTAEPKNPLFNQTLALALIRRQLPADRAEAIDLLTRFGLPHE
                     LDLLGQLALTLEAQTRPWPAHSPAAEIPWPNGLPSPQLDPAPPPDPTPQPIDQPAEQD
                     NLSPSPGTEGRRNSTPLARGKAPAATDLKRLERLLVSHQPLDALQLVGAVLDRGREDA
                     YLHLGGGVAAELAGDPLRARAHLTRALELDPRQMLARAWLGRIYWRSGWWDLAAAVWR
                     SLPVEGPYDHGRHYLLALALDGKSDRKAALAAMATALKEFFYDTFHFSIQDAWRRWRD
                     QAAAPGQG"
     gene            21347..22360
                     /locus_tag="PLS90_00820"
     CDS             21347..22360
                     /locus_tag="PLS90_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00975.1"
                     /translation="MRLTMRRLLSLTLLSLGLPFSGLAAPGDDLARQGNELYHQERYD
                     EAVAAYRQALEKAPDSARIQYNLGSALAQQGQYADAEQVLRQTATRLGSGVQRDAYYN
                     LGVTVAKSADALGQGGGGGAGSSLHQQMAPQMEIARLEEALAAFREAMIVDPRDDAAR
                     YNYEVTLRRIEKLRQQAQQQQQDQSGRQQSDQRNQQQQSGEGDQSQDGQNNQDDTSQN
                     DQGGQQQQEQQEQSQDQTPPDQPEPQQGEANDEQNDESQERQQEGLEGAAPTPQPTPP
                     SERQAEQAGAAAGEERPRELTPEQMDAERLLNLLSEEQPEQFKRLFQFRGQTRPSRQG
                     KDW"
     gene            22360..24231
                     /locus_tag="PLS90_00825"
     CDS             22360..24231
                     /locus_tag="PLS90_00825"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPK00976.1"
                     /translation="MRPLRRLPLLFVIWLALAAPPAPRAQGEPTLHAEVNRQVVGVGQ
                     PFTYTLRIEAGAAGINAKPSIPDFGDLRILGGPSQSSSTSIFNGRVSISQSYTWTLAA
                     PREGTYIIGPSRMRGAGGLIESEAITITVHEQVEPRVVLPRGLPSDQILSARSAVGNT
                     EVDQYLNGRLFLRPQISNTRPYVGEPVIVTYTLYNGARISLGQNTRAIMPDDFQGVVH
                     EVLYSHDHLDYRPEMIDGQRFDVAPLYGMALIPTRPGVVEVGGYSIETRVNLPRGRGG
                     FRDPFDDPMLDFFGGRGEPVQVPTRPIRLEVQPLPDAGQPDNFTGTVGDFTIAASLDR
                     TEANENELVTLSVLLDGRGAIELASAPRFNSEAFELVGETESVNKLNTAEQLGGSKTF
                     EFVLRPRATGRLEVPSIQYPIFDPYRGEYRILRTEPQEITIAAAPNAAQPQTPRSRTN
                     RDEPGEPQLHWIKPITRLETKPAAPLVESAAVWGAQLSALALLLVAWRHDRRRARRDP
                     ARIRREAAPREFERRVQALRREPRDVRAEDLAGRLEQAVRHYVADLHGISADGLTRPE
                     IESRLHAQGLSAERTAKVMELLDACAAVHYAPAPSSREQVMAWADELGGLLRGGAAR"
     gene            24240..25040
                     /locus_tag="PLS90_00830"
     CDS             24240..25040
                     /locus_tag="PLS90_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK00977.1"
                     /translation="MPTLVLLVAAAFAMAQDEDVARAAVVPQTQSENPLAATARDVLA
                     QAEHAFQAGDYERAAELYQSLLEQAPRDPRLLYNLGTAHARAGETGLAVWRYMQALQL
                     DPRDPELRANLRILAPNLKDDLAIAPVPPLNWAYWKLTGNEWTKLAALFTVVALLLGA
                     AAFLRPRMTRGRYALRLVGWGAAVAALLLWAPALLHYQREELSSEAVVVARETWARTG
                     PSENEIETFGPLDHRIVQVIDLTHEGWVKIGLGGGRVGYVQRDAIRFI"
     gene            complement(25137..25222)
                     /locus_tag="PLS90_00835"
     tRNA            complement(25137..25222)
                     /locus_tag="PLS90_00835"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25186..25188),aa:Tyr,seq:gta)
     gene            complement(25324..25400)
                     /locus_tag="PLS90_00840"
     tRNA            complement(25324..25400)
                     /locus_tag="PLS90_00840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25364..25366),aa:Thr,seq:tgt)
     gene            complement(25606..25908)
                     /locus_tag="PLS90_00845"
     CDS             complement(25606..25908)
                     /locus_tag="PLS90_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00978.1"
                     /translation="MAFLAQELHRFDPSREGLTIQTLTQFVESLIPQSRGRSGHESAW
                     NPFRNETSSGFERSGAERERKKPPQPAARRVAVDAEGRNTASADAAYFRIDRDGPA"
     gene            25789..27198
                     /gene="dnaA"
                     /locus_tag="PLS90_00850"
     CDS             25789..27198
                     /gene="dnaA"
                     /locus_tag="PLS90_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPK00979.1"
                     /translation="MTRTASALWDQALDELRERLDRESFTTWIEPVQLLRQEGHVVQI
                     GVPTLFYRNWLANNFHEPIQDCLSRLLRADVQLEYEIMNEVDEEEAPLAPGGLSQTEQ
                     RAMEVFRRGRRERDGAQPTDLITCPLNANYTFETFVVGESNQFAHAAAQAVADPESRA
                     YNPLFIYGGTGLGKTHLMQAIGQKLQTYGQHFNVLYVSSEAFMNAFIEAVAQKKMVEF
                     RNCFRNVDLLLVDDVQFFSGAEQTQTEFFHTFNVLYDAGKKIVISSDHPPKELHELEE
                     RLRSRFEWGLIVDIQPPDLETRVAILRRKARMMNIELPPEVTIYIAERIRSNLRKLEG
                     SLKRLSAHSTLTRQPITMEMARTMLGPFLVGEEPRKISIEKVQMAVCNYFNITLHEMT
                     GPNRARKFAMPRQIAAYLCRELTDQSFPEIARKFGGRDHSSIIHSHRKIQKDMAVQLS
                     LQNLIKYLTKTIKEEPAGH"
     gene            complement(27499..28980)
                     /locus_tag="PLS90_00855"
     CDS             complement(27499..28980)
                     /locus_tag="PLS90_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK00980.1"
                     /translation="MLPGPARLFRPARYSVILLLSLAALISAQEADEPAPKTGTAEAR
                     FGLTFEPVQFMPPPVHHLTFENGIMFYHRPERTIPLVTVLVGIRAGSVNDPPGKVGLA
                     SLTAEVLRTGGSAALDGDALDVALESRGAQLSAESDEEMTWIRLSVLPEDAEWGLGVL
                     RDLLTAPAFPQPRLEEAAGRFAVALRQRLDVPMNVAQALFPQLVYGKGNPWGWTATEA
                     TLAAITPADLHAFHERFYTAGNLRAAVIGDLDREEAERLMRSTLGQLPAEPAPGIDLP
                     AVEPVQSSRVYIVPRDVTQNVIYFGHEGIGRFDERKFAAKVLNSVLAGGFTSRLFKEV
                     RSNRGLAYSVFGRLGEGTVAGLYFNVAMTRVEATSETLGLMLDINEALTQAPPSAEET
                     QLAIQSDVNAFVFFFDTSEQIVRQAMILDEFGYPEDYLETYVDRLKGVESEQVRQAAA
                     ANLHLDRLVVLIVGKVDEDFRRELERIGPITEISDEELRAEWL"
     gene            complement(29111..29632)
                     /locus_tag="PLS90_00860"
     CDS             complement(29111..29632)
                     /locus_tag="PLS90_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HPK00981.1"
                     /translation="MIELAFAFLSLLGFFVLIVVGLFGGTMTERRHLRRLAEAEQALR
                     PMLCTDLRRFPGRAPGAVGGTLVVGQVVIASDYLKTFLSKWRLIFGGEIRSFRGMMTR
                     ARREAVVRMLSEARARGFDAVCNVRFSTADIGGMTTSEKANVMVEVVAYGTAYHARPD
                     ERIHAGIVPEVVA"
     gene            complement(29719..30021)
                     /locus_tag="PLS90_00865"
                     /pseudo
     CDS             complement(29719..30021)
                     /locus_tag="PLS90_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
     gene            30291..31256
                     /locus_tag="PLS90_00870"
     CDS             30291..31256
                     /locus_tag="PLS90_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPK00982.1"
                     /translation="METAPLSSLFRTLGDPLRLRLLHILSVEELTVSEIVRVLELPQS
                     TVSRHLKALRERGLLADRPVGSATFYRASLAAELGNGDTLLRDTLATMLRETPLPPAD
                     RARLEQVLALREAEGGEFFDRMAHRWDALREACFGPTFHFEAFLHLLPAPWRVADLGT
                     GTGYLLPALGRRFRKVIGVDRSASMLELAGRRVAATGLTNIELRVGELEGRLPLRAGE
                     IDLAVAMILLHHLHDVAAALRSIHRALKPGGRLLLVEILPHENEAFRARMADRRPGID
                     PDQMRRWLTEAGMREIERWDYPFDAHPEHELAPLPGLYGMTAVKG"
     gene            31358..32785
                     /gene="ahcY"
                     /locus_tag="PLS90_00875"
     CDS             31358..32785
                     /gene="ahcY"
                     /locus_tag="PLS90_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012969559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPK00983.1"
                     /translation="MTEIPPYKVKDLALARWGREEIRMAEHEMPGLMATRREYGPRKP
                     LAGARITGSLHMTIQTAVLIETLLELGAEVRWASCNIFSTQDHAAAAIAERGVPVFAY
                     KGESLQEYWQYTKWALSFEQSGPTLIVDDGGDATLLIHRGADRELEFEQTGRKPAVCT
                     DNEEIEIVDRLLNDTLDENPRLWRTMAEGIVGVSEETTTGVHRLYEMERAGTLRFPAI
                     NVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKKAVVLGYGDVGKGCAQAFHGMG
                     ARVYVTEIDPICALQACMAGFEVVTMDEACKWGDIFVTTTGNVGVISRRHMDQMKHEA
                     IVCNIGHFDSEIDVASLTRDKSLKWHEIKPQVDQIEWPDGKRITLLAKGRLVNLGCAT
                     GHPSFVMSNSFTNQTIAQIELWTERASGKYGKKVYVLPKQLDEKVALLHLDQLGAKLT
                     RLTEQQAKYIGVPVDGPFKPDHYRY"
     gene            complement(32787..33035)
                     /locus_tag="PLS90_00880"
     CDS             complement(32787..33035)
                     /locus_tag="PLS90_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00984.1"
                     /translation="MTFTIEYEQEDDGRWLGSHASHDDQDLRERTWSLPFLLIDHFVC
                     DEICHSKRKPSSRACPTTVKKSPPVSVVPADLHCLQLR"
     gene            complement(33234..34097)
                     /locus_tag="PLS90_00885"
     CDS             complement(33234..34097)
                     /locus_tag="PLS90_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPK00985.1"
                     /translation="MKSVFVAYDGSPSAEAALRYACHLASLFEGKIYAGYVVELTNDP
                     AVTTGMMTASMEYLAAMPPSRSPEEIEAERQERLAHAQRLFERVQAICQEWPVPFETM
                     VRTGYAEEEILARGAMVDLLAIGKGSPEPEDPERRRLSRLARAIVRSAPQPVLVAAEA
                     YHPPRDIIILDNGDERALHALAFGARLAGLAGLPLRIVTAAHDDADAQWINERARRYL
                     ADHQLEFTFDAIPARFEPADEIIQHLAQYPDALVLMGAFGDSRVKTLIAGSTTCAIMK
                     ALAHPILFFRY"
     gene            34263..35894
                     /gene="dacB"
                     /locus_tag="PLS90_00890"
     CDS             34263..35894
                     /gene="dacB"
                     /locus_tag="PLS90_00890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPK00986.1"
                     /translation="MRRFIPRSPARRCPPALHIALALFALLALPGVPRAQPTAPGTTY
                     ELLGERLQRRFEMMDFGGGRLAALVTDARLPIVAYARDEHQPLAAGSLIQILTAAAAL
                     DWLGPEFQFKTELRMTGNRAGRTLNGDLIVIGDGDPTIGSVHRRPPPKRKRPGFLLLR
                     GAARLVPNEAAACELFDDWTAQLRQRGIRRVEGRVIGDAARFDGEGAAPGWPMRQRGS
                     ARLPTISALNFNDNCLDFFWHASNRIGMLADFSLFPRLPGYNHVSNNVRVQMVSPAER
                     RYERVEVGNLIAAIGSLSPASVAHDRAAIASPPEFFAVALQRRMEARGIAFGGEPAAA
                     GNLSPGELSPAGELLATHLSPPLPEILADMLRHDAALHAECILKTVGLAESQAETGGS
                     FAAGVRAVQTMLRRQRVGGSPPTLLDGSGRSTLNRVSPAHLVRTMQAMLTGPQGQVFE
                     RLLPRAGDAGVLEGHFQGRGDAAAAEPVDAKPPVWAIPAAGEGYQALAGWAEARGGRR
                     FLFAFMVEGSALPATVLRDNLDRLVLEITRLPTQP"
     gene            35891..37681
                     /locus_tag="PLS90_00895"
     CDS             35891..37681
                     /locus_tag="PLS90_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK00987.1"
                     /translation="MSPANAAPSRPPRAGFRGRARRTLYWLLPVAVYLGCALHLPRNQ
                     GRDLVPTPDAVEYAMLSQRLARLQPPLIDTGLQLYPSRYPLAFPLALAPFNWLAGFDL
                     TRLHWAATCYGLLSVLLMARVGAWLLGSRTAGGLAALFWALHPALLEASAVVMSETAI
                     VAAWLALLQVGRPWLEPGGPAPGLARGIAAGLLAGWLTVAKAPFAYWALVFAILVWDR
                     ALARRRFAAAIGLTLAGLLCAFGDALYRHWAFGDWRLNGYQFWHPVIYSEFRRTFNPR
                     YALEGYVGYGGGIGNLVYYARHFLGLTGAYYGRYAAPVAAAALACLLWPRRRGRPWGR
                     VVLILAGWAAVGALFLGTYFFLEYRLGLLWLPLIDGLAAWGLMRAWTWSRLRRGPFLR
                     WRAHRWAQVLAIFAAAILIRSAARHAYPVMHPPPGQVRASQVDSARRLLAHVPADAWL
                     FSGWEAGVVNRIRPRPGPTAGLYSWIVDAPVMNGHLAKALEFDLRPVGITPLGPAGRE
                     ELPAAWRREPAVLLIAAGAGDAAHWTISPAERERLFHRPAYILAQAPPWFPSTAEFLN
                     KVVLPTLARELHLEEVARHGDFVLYRARAE"
     gene            complement(38142..39161)
                     /gene="meaB"
                     /locus_tag="PLS90_00900"
     CDS             complement(38142..39161)
                     /gene="meaB"
                     /locus_tag="PLS90_00900"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPK00988.1"
                     /translation="MPDSAPSRPPVRRTLSLEDYRDGIRRGDRAILGRAITLVESSRQ
                     DHQYLAQELLSAILPDTGNSHRIGITGVPGAGKSTFIESFGGMLTGKGHRVAVLAIDP
                     TSSVTGGSILGDKTRMVELAADPRAFIRPSPSAGTLGGVTRKTRETMLLCEAAGFDVI
                     LVETVGVGQSETVVYDMVDFFFVLMIAGAGDQLQGIKRGVLELADMLAVNKADGDNQL
                     KARQAALEYRRAFHLMRPLSPHWTPPVVTCSGLKRQGLEEIWELIREHRRKLTAAGEF
                     EGRRRRQRIQWMWDMVNDQLGIALRRHPSVQSRLPAIEQAVLHGTLSPTLAARHLLEA
                     FGLSR"
     gene            complement(39558..41756)
                     /gene="scpA"
                     /locus_tag="PLS90_00905"
     CDS             complement(39558..41756)
                     /gene="scpA"
                     /locus_tag="PLS90_00905"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPK00989.1"
                     /translation="MSPLPDFTKVELSEPTGGGDLARWQREAEQLAGRRLEDVTWTTP
                     EQIDVKPLYTSSDLDGVTHLDTMPGFPPFLRGPYATMYVMRPWTVRQYAGFSTAEESN
                     AFYRRNLLAGQKGLSIAFDLATHRGYDSDHPRVMGDVGMAGVAVDSIEDMRILFDGIP
                     LDQMSVSMTMNGAVLPVMALYIVAAEEQGVALKQLSGTIQNDILKEFMVRNTYIYPPE
                     PSMRIIADIFAFTSQHMPRFNSISISGYHMQEAGATADLELAYTLADGVEYVRTGIKA
                     GLDVDAFAPRLSFFWAIGMNFFMEVAKLRAARLLWARLMKQFNPNNEKSMSLRTHSQT
                     SGWSLTAQDVFNNVIRTCIEAMAATQGHTQSLHTNSLDEALALPTDFSARIARNTQLF
                     LQQESGTTSVIDPWGGSYYVEKLTADLAARAWSHIQEIEELGGMAKAINAGVPKMRIE
                     EAAARTQARIDSGRQTIVGVNKFRLDQEERIEILKVENSAVREAQIARLKKLRAERNQ
                     AAVEHALDALTQAAAEGTGNLLELAVQAARARATVGEISSALEKVYGRHQAAIQSIQG
                     VYSGELGEEEGTVSRIRQLVSSFIEQEGRRPRILIAKMGQDGHDRGQKVVSTAFADLG
                     FDVDVGPLFQTPEETARQAVENDVHIVAVSSLAAGHLTLVPQLRAELAKQGREDIMIV
                     VGGVVPPQDYEALYQAGAAAIFGPGTVIGEAAVELLEKLSGHLGFALPAK"
     gene            complement(41753..43903)
                     /locus_tag="PLS90_00910"
     CDS             complement(41753..43903)
                     /locus_tag="PLS90_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009097271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPK00990.1"
                     /translation="MSNPSLTEQFPPVSYEAWRRVVEQDLAGASFEKKLVTQTYEGVS
                     IQPIYTRRDWPVEGDRSGLPGQPPFTRGARPVNRRPLALDVAGWDIRQEHRHPSLAVT
                     NQAVLADLERGATSVQFRLDAAARAGLDGDDPAAGDLAGQDGVMIYSSADLGRALDQV
                     LLEIAGIGLDAGAQFLPAAGLLVALLRERGLDPARARVAFNADPLAALAEEGRLPTRL
                     DEALDRLAELAAWTSRHMPSSRSVCVGTSCYHRAGATAVQDLAFSMATGVAYLRAMLG
                     FGLTLPAALNQLLFSYSVGCNQFLAIAKLRAARRLWARVAEACGAEPRDRAMALHVRN
                     ADRIMTVRDPWVNMLRTTVCCFAGAAAGAESITITPFDSQVGLPDDFSRRIARNTQVI
                     LMEESHLGQVMDPGGGSWYIERLTEDLAAAAWKLFQEIEARGGMGEAIVSGWVKEQIE
                     AAYQPREKNLARRKDAITGVSEFPNLGERSLEPREPDRAALREEARQRLEGLRRRGNV
                     GRALQALEAQVRVREGEPGELMETIIADAQAGATIGELAQSLDGGHEAVTIDALVFHT
                     FSEPYEALRAASDEYAARAGHRPSAFLVNLGPVAQHTARAGYARNFLEAGGFEVIDGE
                     GCDDAAAAGAAFAASGAGLAVICSSDAVYQQLAAPVAGELKRRGARRIVLAGRPGENE
                     ASWREAGIDTFIHIGCDVLGTLRSLLKEQGVEVE"
     gene            44361..>46333
                     /locus_tag="PLS90_00915"
     CDS             44361..>46333
                     /locus_tag="PLS90_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HPK00991.1"
                     /translation="MDMNKLTQKTQEALAAAQTLAIEHGHQAVDVEHLLAALLDQPEG
                     IIPRILERADCDVDIIRKNLDAELDRMPRVSGPGVEPGQVYVTQDLNRVLVRAEEEAR
                     RLRDDYISVEHVLLAMLDSAKARPAGRVLQKSGVKHDAVLRAMAEVRGNQRVTSNNPE
                     ATYEALTKYGRDLVEEARRGKLDPVIGRDEEIRRAIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAHRIVRNDVPDGLRNKTVFSLDMGALLAGAKFRGEFEERLKAVLAEVQKAEG
                     RIILFIDELHTIVGAGKAEGAIDAGNILKPMLARGELHCIGATTLDEYRKYIEKDPAL
                     ERRFQPVLIEEPSVEDTISILRGLRERFEVHHGVRIQDSALVSAAILSHRYISDRFLP
                     DKAIDLIDEAGAMLRTEIDSMPSELDQVSRRVMQLEIEQAALRKEKDEASRERLAALE
                     KELAELRDEQSRLNAQWLAEKESIGKLRELREQIEQTRAAVERAERAYDLNKAAELKY
                     GKLRDLEQQLAALEKQQHDKLGAGTLLREEVTEEEIAEIVARWTGIPVTRLMEGEREK
                     LLKLDEILHRRVIGQDEAVRAVADAVLRGRSGLKDPRRPIGSFIFLGPTGVGKTELAK
                     ALAQALFDTEENLVRIDMSEYMEKHSVSRLIGAP"
BASE COUNT         7783 a        16595 c        14104 g         7751 t
ORIGIN      
        1 ggcatcgacc ggggcgacga ggtgatcgtc cccgaccagg cggcgcgcga cgccgacccg
       61 gccaaccctc aggcgtagcc cttcgcccgt tcctcgctca gcgtgtcgag ataggcccgt
      121 acctgctcgc gccgcatcgg cggaatgccg ctctcggtgc gcgggatgaa ccgccctccc
      181 cggcgaccca cggccatcac ctcgcgcacg tgcgaccgga tttcctcttc actcatgaac
      241 tgcatccgcg tgctgatccc cccgtgcagc acgatccggt cgccccagcg cgccttgaac
      301 agggccagat cgttgtaatc cgacggcccg accggattga tcatgtccac gccggccgcc
      361 acgatcccat ccatcaagtc ctgaatgtgc ccgtggctgt gcagatgaac ccaggcgccg
      421 cgctcatgcg ccagatcggc gaccgccttc aaccaggggg aaacgtattc gtcaaacagc
      481 gggggagcga agatcagtcc tcgcccgctg cccagatccg agccgacgaa gatggcgtcg
      541 acgccgcaat cgagcagcac gcgcgccgat tcgaccgaaa accgcgtcag catgtcgaac
      601 agcgcgtgcg ccgcctccgg ctcggtcagc atccagcgca gcgcgtcgtc gaatcgcgcc
      661 agatagccat agaccgatcc ccagccgccg atgcaccacc cttcgacaaa atagccggcc
      721 gcgtgatacc ccgcgatccg gcgccgcagt tcctcggcgt cataccgccc gggggcatgc
      781 acctcgggaa actgaaactg caacgcgtcg cccggcgatt cgatcggaac cttgagcacg
      841 tgccggttga aaaccggccg atattgctcg cgcacgacca gccccgtctc ataccgatag
      901 acccggtgac cctcatcctg gctgagcgtt tcacaggcca gcttgatccc cacgccgatc
      961 gcggaattgt cgaagcattc ggcgaaggcg aattcgtccg ccacctcgtc gcccgtcggc
     1021 ggcggcgcca gcagcttcgt cgccgtcacg tccatcgggc actcatagag cagcgggatg
     1081 cgatcgaccg gctgaccgag gtaaacggcg cggacacgct cgcggtgcga cagggcgacg
     1141 gcggcggacg gcatcctgcg actcctcccg accttcaacg atggcttcaa tgactgcgtt
     1201 acaacgccag ctgccgacgg atggcatcgg ccgtcgcggc gtattcggca gcgctgagca
     1261 gccgcggttc atgcggttca tcccacggcg tccgccgcca cagaaaatcc cacgtgtagc
     1321 gcggaatcag atgccaatgg atatgctcca ccaggttgcc caggcattcg taattcagct
     1381 tgtcgggatg gaacgcggcg tcgatcgccc gcgccgtctg gatcatttcc tcgacgtggc
     1441 gtccccgcac ggccggatcg agctggaaca actccgttgc gtgctcgcgc agcgccacga
     1501 cacagcgtcc cggccagaac tgatcgcggt gcagatagat gatcgacgtg tcggtctcga
     1561 tcagcggcat cagccagcgt tccagcggtc ccgttccgtc gcgcatcaac cggcagtact
     1621 ggcagtccgt gtcatactgg gcctgcacct gcagcgtcgc cgccgacggg ctcggatgcg
     1681 atgggggttt tgtcatgttt ctccgtcctg ttcgatttcc gtggtgcgtg caatcccggc
     1741 cgccgtgtac acccgtccgc tcaatgcctc cgatacgcct tgacgatcga ccggtacagc
     1801 tcatagttcg cgaccggaat atccggcgcg atcgaatgcg tgcccatcac caggccgccc
     1861 aggcgccccg cgtcgatcaa ggtctcgacg tgacgccgaa tttcccgctc ggttccacgc
     1921 gggaggatca cgctgttgca gaccccgccg atgaacgaga gccggccgaa atatcggggc
     1981 agcaattcct cgacgatcag ccccgtccgg cgctcgaccg gattgatgcc attgaatccc
     2041 gcctccacga cgaggtcgag caacggcccc agattgccgt cgctgtgcag gaagacgcgc
     2101 cgcgccccga ccgccttcaa ccggccgata atcgactggt agaccggcag gaacatcgac
     2161 tcgaaaatcg ccggcgaaaa catcgggctg cgctggttgc acatgtcgtc gaagatccac
     2221 accccctgcg catacgcgtc ggcgcgccgc agcacctcca gccccaactg caactcatgc
     2281 tcggcgagcc gctcgatcag cgcgcgcgcg aactccggat ccgacaccag atccatcaga
     2341 aattccgctt cgccccgcag cattgcacag cgattgtaga ggccgccgat cttcacgaag
     2401 accggcttgc ccgccgcgcg cgcgccggcc acctcgcggt cgaatgtctc gaaccggaca
     2461 tccagattca ccgggtcgaa cgtcagccgg tccagatcgg acttttcctt cagatcacgg
     2521 tcgatgacat ggcagaagaa gccgctgcga cgcgtcagcg tccgccgtcc ccacccgtca
     2581 tccgcgacga cggcgtcgcc gtcgtcctcc agcacccgtc ggtgggtcac gcagggcgcc
     2641 tccagcgcgg cgcatacggc caggtcgagc ggaaaatagt ccagcacatc cgccgattgg
     2701 tccagcccgc gctccgcccg ccatttcgcc gcccactccg gccagatcgt ctcgaacacc
     2761 ggcatgcgct cgggaggttc aaacgacaac gcctgttcga cgatccgcgt cgagtcgcca
     2821 acccgcgcaa cgggtatcat cataccgcgg cctgtgctca tccccccatc ctccccccac
     2881 tcagaatcgt ctctcgtcct cggctctcga aaaccgtccg gttccctgcg cccaacccac
     2941 tgcgtatccg tcgatcatgg ggcacagacg gcctgattgc aataggccca aacgctcccg
     3001 gcgaccccaa tcgccagtct gcgccgcgtt tttttctggc caaatcctga cgatgaactc
     3061 ataatccaat tctcatccct gcacggccaa gttgcccgtt acatatcgtt tgccggccaa
     3121 ctatccgctt cgcttcgctc gtgccgtccc tttcttcgat tattcggtct cccccacgga
     3181 agaacgagcg ttgctcaaga ccgtctgcca cagccttctg atcccctttc tgtgcattca
     3241 actggcgctg cccgtctggg gcgcgcagtg ggtctggcag aatccactgc cgcagggtca
     3301 caccctctcg tcggtccagt tgctcgatga ttcccatggc tgggccgccg gccgcgacgg
     3361 cgtcctgctc cataccctgg acggcggcgc cacctggaac gtcatcctga ccgatggcga
     3421 cctcgcgttt gaagacttcg tcttcctcga ctcaagcaac ggctgggcga tcaccggtat
     3481 cccctatcgc ctgctgcgca ccaccgacgg gggccgctcc tggaccacgg tcccgacggt
     3541 cgataatccg cctccgctcg atgacctgca ctttcatgat ggccagcatg gctggatcgc
     3601 ctccgaaacg caggttttac gcacgaccga cgccggcctg acctggcacg cccacgagac
     3661 ccacgccagc ggcacgctgg ccgcgatcgc gttccccgat cccctgaacg gctgggccat
     3721 tgccaatcat ttccccaacg gcgcgtccat cctgcacacg accgacggcg gcctgagctg
     3781 gtcgccgcaa accgtcgccg tcagcttgga cagcactcgc ctcagtgccc tccactttgt
     3841 cgatgcgatg cacggctggg ccgtcggcct gggcggccag atcctgcata ccgccaacgg
     3901 cggcgccgat tggtcgcccc agtcgagcgg cctcggcatc cccctgagtg atgtattttt
     3961 cattgattcg atggtcggct ggatcgtcac cagcaactgg acgctggagc acggctatca
     4021 ggtcctgcac acgaccaacg gcggggccga ctggtcgccc caggccaccg gcgccgagca
     4081 agccaccaat ctgctcgcgc tgcatttcgc caacgcgacc gatggcctgc ttgtcggcga
     4141 atccggcatc ctgttcaaca ccatcaatgg cggcgccgac tgggtcgctc gctcctccgg
     4201 cccgaatgat gacttcctcg acgttgactt ttccgattcc cagagcggca tcgccgtcgg
     4261 caccggcaat tacctctatc tgaccgaaaa cggcggcaat tcctggcaga acgtcccgat
     4321 cccgccggag gattcaaagg agatcgcgcg gctcacccat gtgtggaagc tcctgagcaa
     4381 ttactttgcc ggtctggtgg gcgcgaccaa gaaagtgatc aaggtggaag acaagaacaa
     4441 gggcaaactc gaggccggcg acctgacgtt catttacctc gatccgggcg ttgcgggcga
     4501 tatcgaagcc ctgtatgggg tcgacgacct gacttggtgg atcgcgacca acgccggtga
     4561 aatcaggcgg accaccaatg gcggtgaatc atgggatccc caggcgtccg gcgtcgagcg
     4621 aagcctgaat ggtttccatt tccacgacgg gctgatcggc tgggtcgtcg gcgaacaggg
     4681 cacgatcctc aaaaccaccg atggcggcga caactggttc cctcagccca gcggcaccgc
     4741 ctccaatctc aacgccgtcc atttcttcga taccaagcgc ggcttcgccg tcggcgacgg
     4801 cggtcgttac ctcacgaccg acaacggggg cgacaaatgg gagatcggtt cgctgagcgt
     4861 cttccacaac ctgcacgaca tcgcgttcgc cgatgccctg catggctgga tcgtcggctc
     4921 ctccactcgc gccctctata ccgacgacgg cggccagacc tggacgtccc agcccaccgg
     4981 cgccagcggc ctcatctggg ccgtcgacgc ccccgatccc gaccatgcct ggatcgtcgg
     5041 cgccggcggc gccatcctgc actacgacgg agcgaccgat tcctccgccg cccattggca
     5101 gctgctccgc tgaaaaaaaa gcttcgaaat ccgccggaac cgtccgggcg gacggatccg
     5161 acagatttcg aagccgaatc ctcgccgggc gcctagactt gctcggtcgc cgcgatttcc
     5221 atcagctcca gcagctcgcg cgcgacttcg gttcggttcg ggtcggccgc atacagtgcg
     5281 ccgacccgct cccgggcttt ggcgccgtcg cccgcggctt tcccccgcgc gatccataac
     5341 cggcaggccc acggcatcgg accccagttg ccgcattcaa cgaagtcctg gctcaggaag
     5401 acgaatttgg ggatcgcctc gccgccgttg gtcagaaacc gcgcaaagac gtcgggatgt
     5461 tgctgccgcg agatgtaccg gacctgcaca cggtcggtcg cttccgccat ccgctgcagc
     5521 accggcacat gccgcaccac atcgccgcac cacgcctcgg cgatggcgag cacatgcacc
     5581 gtcttgggca acgccttcag cgccccctcc accggcgccg ccagacgcag cccccggcgg
     5641 tcctcatcca tcttcttgca gttctcggcg tattccttgt tcgtaatcca ctggtcgtag
     5701 ctttcgccgc tggcgaagac cttgggccat tcgatcagcg gcagtgtcgg cggcatcggc
     5761 atcggttttc tctcctttcc agagggcggt cgttgaatcg ccccaggtgg agcgcggcgc
     5821 aaccccgcgt ccgcggctcc caaatcccgc cgttaccccg cgatgtcctt ccagcgttcc
     5881 cgcagttgat tggccatcca caggcaaaca ctccacaagt cggccaccgg cacattggtg
     5941 tagggcagcg tgtgcagata cggcggcggg ccgaactccg gcgtcacggt caacagctct
     6001 tcgccccgct cctcatgatg cttgcggatc cggtcccact gcttcagatg ccactccagg
     6061 taacgcgccg cctccggcgc ggccgggtcg ggcacctgcg ggccttcttc gtaacccacg
     6121 cgcgcatgga tgtgcagcga gcgttcgcag gccagcgcca tggcctcttc ctggtcgtcg
     6181 ggcagccgtt cgcagacgtt gacccagtga ctgaagtcgg cgttgacgcg cagcgcgggg
     6241 aactccttca tatagtacag cgcgtcccac ggcgaataga acatccgccc gcggtgcgtc
     6301 tcgatcagga tcggcatgcc gatctcctcc tgcagcgcca gcacccgcgc gaagtagcgt
     6361 ttgccctcct cgcggctcat gcaatcgcgc cccgcgtgca gccgcatccg cgtcggtttg
     6421 agcggcagca acgattcaac ctggcgcgcc agttcatccc cgtcgacggc cgacatcgcc
     6481 gcgatgaaat ccatcttgtg atcggacagt accccgcggt actcgtcgat cggcaggtcg
     6541 ggcgccagcg ccgattcgat cccgtcgtag ccggccatcg ccgccgcttc gatcttcgat
     6601 tgggtcgagc cggtcatccc ccagaacgaa tggtagattc gcaattgcat cgtcagattc
     6661 tctcctcgaa atagatgacg ctcaggtaga gtttcggccc catcaataca ggttgccgcc
     6721 gcgaagtcaa tcaatctctc cgccgcggcc ccccgcgccg ccggcatggg gcttctcttg
     6781 ccgcgaattg agccgtgccc agcgcatcca actcgcaaat ccgtcttatc cgtcggagcg
     6841 aagattgtcg gcggaatccg cttccgccgg cccctgccgg ctctgccgct tacacccgct
     6901 cggcgacggg ccgcggcgtc ccgtgcgtgt gcggccagag gtgccccagc cgcttttccc
     6961 cttcgatcgt cgtctcaaaa tagactgcca gcaccggctc ctcgaccttc ggaaaatcgt
     7021 gcagaaagcc ccggccggtg gcgacgcagt cgcccggacc cacctggtag tgcttgcctt
     7081 ccgaaacggc ttcgccccgc ccctccagca ggatccagta ttcgtcgcag tcgtggtagt
     7141 gggcgtcgaa cgtggtggtc ttttcgtaac tgaccggccc gcccgggttg tcggtcttgt
     7201 cggtgttcac cacgctgaac aggccggagc cccccacctg gtcgccccaa tggcccgcca
     7261 tgcgcgtcag aatcaccggc tcggccacct cggtgacttc gaaattcagg cctggctccg
     7321 tcagatcaac gatcgtgccg tgttcgagca tcttctcttc acggccgatc cgcactttgc
     7381 atttgccctt ggcggcgatc agtttctcct tgcgcccttc gcgagtaaag ctgtgggtct
     7441 ggcccggctg gagccggacg atgtcgaatt gctccagctc gcaccacgcc ggcgcctgac
     7501 cttttccgct tctgaagact ggcatggctc atgactcctt gctgagggtg atgccgatcg
     7561 ccggcagggc gcagtcggca ttctcccgca ggatagagag ccgcccgggc gaaatccatg
     7621 gggatttgcc ccatcacccc caccctcaac tcgtcacgcg cagcacttcc tccacggtcg
     7681 tccggcccat gaccaccttg accagaccgt cctccagcag ggtgcgcatg cccagtgcgc
     7741 gcgccgcgcg ccggatctca taggaaggcg cacggttgcc gatcaactgc cggatcgtcc
     7801 cgtccggccg cagcacctcg tagatccctt cgcgttcata gaaccccgac tcatggcagc
     7861 gctcgcagcc gcgcccgcgc ctggccttga tcttcgccgg cgcattgggg atgccaagct
     7921 tcgcgaagta gtccgcatcg atttcaatct cctcctcgca cgtgtggcag atccgccgca
     7981 cgagccgttg cgcgataacc atcagcagcg cctccgcatg gcggtgcggg tccaccccca
     8041 gatccagcag tcggccgatc gtcgtcgtcg tgtcgatcgt gtgcagcgtg gaaaagacga
     8101 ggtggccggt catcgcggcg tcgaccgtca tcttcgcggt ctcgccgtcg cggatctcgc
     8161 ccaccataat cacatcgggg tcctggcgca aaattgagcg caagcccgtg gcgaaatcga
     8221 aattcttcgc cgcgttgacc tgcatctgcg tcagcccctc gatatggaac tcgatggggt
     8281 cctccagcgt gatgatgttg cgccggccat cggcgatcgt gccgagcgaa ttgtagagcg
     8341 tcgtcgactt gcccgatccc gtcggcccgg tcaccaggat catcccgtgg gggcgctgga
     8401 tcgcgtcgac cagcagcccg tgcgcgtgct tcgacatgcc gaggctctcc agcgaaccga
     8461 gcacctggtg gcgatacagc agccgcatcg acgccgtttc gccatccccg gccgggctcg
     8521 tgttgacgcg cacgtcgagc ttccggtcgt cggccatcgt tttgaaactg ccgtcctggg
     8581 gcttgcgccg ctccgcgatg tccagattgc actggacctt gatcgttgaa accaccagcc
     8641 gcagcaactc ctcgggcagc tcgaccatcg tccagaggat cccgtcggtg cggcagcgga
     8701 cgaggaattg ccccccctcc gaaatcagat gcaggtcggt cccgcgctcg cgcacgccca
     8761 tcgtcaggat ggcgcgcagc atccgctgca ccatgtcgct ttcctgcttt tcggcctttt
     8821 tggccgcctt gcgcttgccc gccttgaact cctgggcctc cagcaccatg tgcgcgtagt
     8881 gcacgatgta gtcgcggatc gaaaagacgg gggggatcac ctcgccgcaa tgcgggcagt
     8941 tctcgaactg cagcgattcg tagcgcccga tgtcgtcgat cggccgcatg cacgaagggc
     9001 acagcttatg cccttcgccc ttacgaccga ccgcgggcga tttcgaccgc ggctgaatgc
     9061 aatacatcag cagccccagc agaccgagcc agcgtacctt gggctgcatg tcgacggcgc
     9121 gcggctcccc cgcctcgacc agccgcgcca gccacacgct cggcagaaac cagcccagga
     9181 aatagagcag actgactccc agggcatacg cggtcgccgt cgccgcatcg agcggcactg
     9241 cgatcagccc ctccagttcc gcaatgtcct cggtgaaccc ggtgccgatc actagcagaa
     9301 tgccgagcgc ccaccagcgg taaaacgtca gcgcggcggt ggaccactcg cccggctcaa
     9361 tccccttgcg cacgcgtgtc cgcgcgatca gcagcggaaa gccccacagc acaacgccga
     9421 ggaccgccgc gccggcgaac agcccgacgt aggtcccctg gagcgtctgg ttcaataatt
     9481 cggcccagtc gaccccctgc aacgtctggc tgtagtcggt caggccccac gcgaagtaga
     9541 tgaacaccgg cagcatcagc aaccgcagca acttgaagct ttgctggcgc agccagacgg
     9601 gcaggtcgtc gtggagcagg ccggtcaccc aggcgcgctg cggcgcccag acaaaaaacc
     9661 agatccagaa gctaacggcg tagatgacga tgggtagatc ggcggtggaa acgggcgaca
     9721 tcggcgcggg agattccctc ggaaggctgg cgtcggcagc ctgggcttct caacctcgcg
     9781 gacggccggg aagcggggcc gggcgcttgc atcgttggga catttaagcc agaatacgga
     9841 ccttgtcgca agcgcgatca gccggtttgc gattggcaat ggtcggttta ggccgcccgc
     9901 gcgcccgacc ccgtcgagcc ggtccgggcg tcgtccagcg gcctgaatcc tgggatgggg
     9961 gtgccgttag aggtgttgcc gcggattctg acgccgctgg gcgcgctgct tatcgtcatg
    10021 ctgacgggct gcgcccgcca acagatcatc ttcgtcaacg cccagcataa ccggttgggc
    10081 gaaccgcgac tcaccgtcca ccagccgggc cattaccgcg tatcggtgcg gcgcatggcc
    10141 aacgcacccc cctggcagga tctcgtcctg cgcaacgacg ggcgggaccc cgtcgatgaa
    10201 atccggctct ggggcctggg cggcgccgac ctgctggaag tcgccaggcc ggccggcgcc
    10261 gaacccccgt cacccgatcc gctggccgaa cccgcgccgc gctcggtcct caagccgttc
    10321 cggctcatgc cgggcgagcg cttcgtctgc ggcgagcgcg aaatcgacgg cgacatgcat
    10381 cttcagggcg ccttcctctg ggcgatgggg ggcgataaca cccggatcct ttcggccgcc
    10441 gataccctgc gcaacgcccg ctcgggccgc acgacgccca tcgtgctgga tccctcctcg
    10501 ggcgaagctt atgtgcagtg gactttttcc tcgcccctgc cgcacctggg cgggatgctg
    10561 agctgggagg tcgatccgcc cggcgcgcgc cccgaagtct ggatcagcgt tgaccgcggc
    10621 gtctggggcc gcctgcccaa tccgcgcggc ggccatcagt gggcacaacc ggtcgatctg
    10681 accagttcca tcgagggcta tcatgagttc agtctacgtc tgctctttgc ccccgattcg
    10741 gtcggcgtcg agtcggagga tggaacgatc acgctgcgca agatttacct tgagcgcccg
    10801 tttggcggtc ggggtgaact gcgcgaatgg cgccccggcc tgaacgaact cgaattcgcg
    10861 tttcgcgccc acggcaacgc ccagttgacg gtcgagctgc tgaacgcgcc gtaacgcaac
    10921 acctggatcg cctgccatgc tgccctctcg ctataccgcc gtcattcatc cttccggtcc
    10981 gctcgggggc tacgtgcagg cgcccccttc caaaaactac acgacgcgcc tgctgctggg
    11041 ggcggcactg gccgaagggc gcagcctcat ccggcggccc gccaccaacg acgatgcccg
    11101 cgcgctcgtc gcctgcctgc gcgcgctcgg cgcccagatt gaggagaccg gacccgacct
    11161 gaccgtgcgc ggcttcgcca gccgcccgcg caatcccggc cggattaacc ccggcaatgc
    11221 cggcgccgtg ctgcgcctgc tcctcgccgt cgcctgtctg gtcgagggcg aggttgagtt
    11281 cgtcaccgac cacaccgact cgctcggccg tcgacccaat gaggacctgc tggcggcgtt
    11341 gcgccagctc ggcgccgacg ccgccggcca gggcagcgac ggtcgcctgc cgattcggat
    11401 tgccggtggt ccccaacgcg tccgcggcgg cgccgtcaag gtcagcggcg cgcgcagttc
    11461 gcagttcctc agcgcgctgc tctttctcgc gccgcacctg accggcgaca cgaccatcga
    11521 ggtcactggc gccgacgcga cggcgccccc cgtcctcgtt tcgcggccgc tcatcgatca
    11581 gacgctcgat gcgatgcgcc ggttcggcct tcgctgcgac ccggatccct cgggatttcg
    11641 ctttctcgtc ccgggcggcc aggtgggacg cgccggcgac cacgccgtca acggcgattg
    11701 gccgtcggcg gcggcgctga tggccgcggt tgccgtcgcc ggcggcatgt ccacgatcca
    11761 cggtttacag gacgatgcgc agggcgagcg ccgcgccgag cgcctcctcg ccgcgatggg
    11821 ctgcaccttt gtccgtcccg aggagcacgc cctggtgatt cactccaagg ggaacttgca
    11881 ggccgccgac ctggacggcg acctggcgac cgacgccgtc ctcgcgctca tcggcgccgc
    11941 ctgcctggcc gacgggacga gccggttcag caacatcggc aacctgcgcc tgaaggagtg
    12001 cgaccggatc cgcgagccgc tggaagagtt ggcccggctc ggcgttcgcg cccgccacgg
    12061 cgaggattgg atcgaaatcg acggcaaccc cgacggctat gacggcggtg tcgacgtcga
    12121 ttcgcgcggc gaccatcgcg tcgcgcagat gctcgcgatc gtcggcctcc gctgccgcaa
    12181 cggcctgcgc atcacccgcg ccgaatccgt cagcaagtcc taccccgatt tcttcgccga
    12241 tttgcagcgc atgggcgtca aactggacct cgtcccggct tcagattagg cttagcccga
    12301 ctccatcctc atcctcgtga tcgactctta aaggcgcccc ccattcgcgg acccatgagc
    12361 aacttgaccc cctgtagaaa ggtcgcccgc gcatgtcgga ccatcccgcc ggttcgttag
    12421 gcgccccacc catcggcgct ggcgggttgc agtccggctg gcccgcgtat gccatcccgg
    12481 cggcgctgtt cctgagcggc gccgcgatct tcttcctttt gcggccgttc ggcgtgccgg
    12541 ggggcgacag cgagctcctc acctcgcccc agtccatggc gctctactgg cgcgattcgc
    12601 tggtctactt catgcgcgaa ccgctggcgc accagatcgc gctccagttc catctggcgg
    12661 ccggcgacct ggcgctcggc atggcgctga gcggcgcctt ctgcggcggg ctgttcctgg
    12721 tcctgctgtg ggagttctgc cggtcaccga ttgtctgggc cgccgtgctg ggcagttgcc
    12781 tgacgtgggt ctttgtcggc cacctcgaac tttacgcgcc cgtcgctgta acgctcctgc
    12841 tctatttcat gctcctcgtc cgcgcgctgc aacccggctc gggagtgacg ccgttccatg
    12901 ccgcgctcgc cctggcgctg gcctacctga cgcacaagct ggcgctgttc ttcgccccgg
    12961 cgctgctgtg cctggcattc gagcgcgtcg ccggccgatg gcgcatccgg ccatggcccg
    13021 ccggcgcgtg ggaacgggcg ctggtggcga tcatcgcgat gtgcctgatc gacatgctgc
    13081 cggcgctggc cgacatggcc ggcgtccatc atatcctcta catcacgttc aacgagcact
    13141 tcctcgacat gatcacgccg ctgacgccgt cgatggcgcg ccggctcgaa gcgcgcagcg
    13201 aaaccggcat gttcttcctc ttcaccttcg gcacgccgct gcatctggcc cattttttcg
    13261 gcttcatcct cgccagcgcc ccgctcggcc tcgtcctgct ggcgcgccac tggcgccgga
    13321 tccgttccgc cgccgcgctg ccgctcatcg tcgccgccgt catcggcatc ctctggatcc
    13381 tgctctggca tccgcgctgc ggctggcgcg attgggatct gttcagcatg gggaccctgc
    13441 cgctgaacct gctgggcgga ggcgccgccg ccggctggtt cgacgccgac gccgcgaatc
    13501 ctcctcatcc cgcgcactga gatccgtccc tcgtcctcgt cttcggccct cgaatcggcg
    13561 ccgtctccct aatccccgcg ctccagccgc aattcgtcgg tatccagcgc cgcttcggac
    13621 ttcaccgcgg gccgatgcag cgccgtggcg ggcggttcgc ccggcgcgcg ctgcagttcc
    13681 tggaccgggc cgaccgttgc cgcgagcaac tccagaatcc gccgatacga cgtttcgtgc
    13741 aggccgcgac cgtccagaat gatccggcca tccggttcaa tcgtgatctg gaattcctcc
    13801 ggttgcgaca tgccgagact ccgcaaaatg cccccgaggc ggcgcttgcc gtatgctctc
    13861 acagacccgt ggcaggggca aaaggttgca caagaccggg cacgcgcctc acgcgccgcc
    13921 cggcaacagg cccgcatcct ggataaagcc ggcgatggct tccgcggcaa gcccatgccc
    13981 cgcggcggta aaatgccgat catgctgaaa ataaagcggt tcccctcgcc gcgcatgggc
    14041 cgcgcgtgcc agcggccgca ggttgagcgc cggaattccc agatcgcgac cggcctgcgc
    14101 ggcccaggcg aaagggagtt cccggtcata accttcttca cggtgtccgc gcgcaacgcc
    14161 gtaacgcttg acgatgcgcc cccacagccg gtcgtcgacg tcgtagtccg atggaatcag
    14221 gacgacggcg aacgcggcgc cgcgcgcctc gcagcgggcc tgcatcgccg cccattcggc
    14281 ccgcgtcagc gcctcggccg ccggcaccca gtccggcgga acgatgagcc gctcgtcgaa
    14341 ccacatgccc tgatacggat tctcccccag gttgatcgaa cctggaatcc gcatgcgcag
    14401 ccagcggtag aaggcgctgt tccggaagag cggaccggtg ggggtcgtcc gcgcggcggc
    14461 cagcggttca ccgtttacgt agggttcccc cccgtgattc gtgaaccggg cggcatcggg
    14521 cgccgcgccg agccaatcgt ccgccagatc gttctccggg aaaaaggcgt ggatcacaag
    14581 cgtgggcgac gtggcgtcga tcagttgttc caggcgttgg cgtgccgccc gcgtgccccc
    14641 gccgacccgg cccgcgttga ggcattcggt cacgagaccc gcggcccgca gccgcgcctc
    14701 ggtctgttcc acgaacgtct ggccggcgtc gaccccgacg ccgaacgtaa aggaatcgcc
    14761 gaggaacaga atgcgcggcg ccgtcgccgg cgcgggcgga tcgtcatcga cccgcagacc
    14821 ccgctcgtcg atccgcacgt cgaacgcata ctcgaaatag tgccacgtcg ccgcataccc
    14881 cggccggtaa ttccagccca gcacctcgtc gtaaatgacc gaaccgggcg ggaaataatt
    14941 gagcgtcaga tcgcggtagc gatcaccggt ggcgatccga aagccaatct cgactgaaag
    15001 gagtccgaat aagagcgaaa ggccgatcgc ggcgcagccg aagagaaggc ttctttcctt
    15061 gcagcgcgcg cgcatcggcg tcttcgcgtc tctgcgtatg tgaattgaat attccgtaga
    15121 tttcggcgcc accgccctgc ccgcgcttca gccacggtgg ttcagaccga cggctgcgcc
    15181 tcgggaccgg ccagcggatg atgcgggcgc ttttcgcgtt cgcggtcctc ggcggctgcc
    15241 ttcggcatcg cggggggcgg cggcggcggc gtcgcccgtt ccatcggcgg cagcgttttg
    15301 ccctccacca gcatccggaa ctcggtctcg tccagcgtct cccgctcgat cagcgcctcc
    15361 gacacccggt ccagcaactc gctcttctcg cgcagcagat ccatcgcgaa atcgtgggcg
    15421 tcgtcgatca gcttcttgat ctcctcgtcg atgatcgtcg ccgtttcctc gctgtagttg
    15481 cgatcgcgcc ccagttcgcg acccaggaag acctgcccat cgtggtcgcc gaacgcacgc
    15541 gggccgagcc ggtcgctcat gccgaattgg caaaccatgc gctgggcgag accggtggcg
    15601 cgctcgatgt cgttggaggc cccggtcgtg taatcgccga actggatgat ctcggcggcg
    15661 cggcccccca tcaggtggcg caactgcgcc atcagttgct gccggctcat cgtgcggcgg
    15721 tcttcggtcg gcaggatcca cgtgaccccc agcgcccgcc cgcgcggcac gatcgtgatc
    15781 ttgtgcaccg gatcagtgcc cggcgtgaag tgcgcgatga tcgcgtggcc cgcctcgtga
    15841 tacgccgtca ccagcttctc gcggtcggtg atcaccatgc tgcgccgctc gggacccatc
    15901 atcacccggt ccttggcttc ctcgaactcc aggtagccga ccgtctcctt gttgcggcgg
    15961 gcggccagca gcgccgcctc gttgatcaga ttggccaggt cggcccccga gaatcccggc
    16021 gtccccttcg cgatgcgcgt caggtcgacc ccgacttcca tcttcacctt gcgcgcatga
    16081 acctgcagaa tcttctcccg ccccttgata tcgggaaggt ccacgatcac ctgccggtcg
    16141 aaacggccgg gccgctgcag cgccgggtcg agcacgtcgg gccggttcgt cgccgcgatc
    16201 aggatcacgc cttcgttctc ctggaagccg tccatctcga ccagcaactg gttgagcgtc
    16261 tgctcgcgct cgtcgtggcc gccgccgatg ccggcgaagc gcgaccgccc caccgcgtcg
    16321 atctcgtcga tgaaaatcag gcatggcttg gccttcttgg cctgctcgaa caggtcgcgc
    16381 acgcggctgg cgccgacgcc gacgaacatc tcgacaaaat ccgaaccgga aatggagaag
    16441 aacgggacgc cggcctcgcc cgcgatcgcc cgcgccagca gcgtcttgcc cgtcccggga
    16501 ggcccgacga gcagcacccc cttgggtatc cggcccccca accggctgaa tttcttggga
    16561 tctttcagat actcgacaat ctcgcccagt tcgtcgcgcg cctcatccac cccggcgacg
    16621 tccttgaacg tcaccttgat ctccccctcc ttgaccagct tggccttgct cttgccgaag
    16681 ctcatcgcct tgttcccgcc cccctggatc tggcgcatca gcaacatcca caggatgaag
    16741 atcaccagga tcggcagcag gatcgagatc aggatctggg agatgtaggg gttggaggga
    16801 tcggtgtcgt agggaacgtt cagctcaagg gccttctgca ggacctcgcc ctcctggccc
    16861 gccagatagc gcgtcttgta ggtgatcgga gcggcgtcgg aattgttggg atcccgaatc
    16921 tcgctcagga tcacattgcc ctcgtcgacg gccttgatga tgttgccggc ctcggcctgc
    16981 ttgatccatt gcgtcagcga cagatcggtc ggcgtgactt tcttgttgta gtgcatgaac
    17041 aacagcacga agaccatgaa aatcacgatc cagagggtga cggagctgag acctttcatt
    17101 gcatcctgtt actcaaggag atcgggctgc gggattcgac gaggaggacc ggaatgaaaa
    17161 gccaggcgtc agtttcggcg gccgccaaat ccagcctcat gctagcagaa gagccaaaga
    17221 cgggccaaca gctaaatccc cggttggcga ccgagcaaac cggcatcagg gccgcttcgg
    17281 ccgccttctg aatcaacatc accgtccctc gtcnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17401 nnnnnnnnnn nnnaccggca tcagggccgc ttcggccgcc ttctgaatca acatcaccgt
    17461 ccctcgtctc tcgtcctcgt ctctcgtcct cgcccccaag ggcccgcccc agaattcggc
    17521 tctcggctct cgaaatgaag ttccatctca ctccctcgtt gtccaaaccg ccgcgccggt
    17581 cgcgcgccgg gttgatttcc cgtcggttct gctgataatt ggaaccccag tgggattctc
    17641 gtccggctca aggagtgtaa tcatgacgtc gagctcgccg cggatcgtga tggcgtgcct
    17701 gtcggcaatg gcgttgatct cgtcggtcgc ggggtgcgcc tccggcggcg cgtcggggga
    17761 acgggcccag ttccagtcgg ccgcgccggc ttcccccgtg cagcccgtcc ccgcggccgc
    17821 cgccggcaag gcgagtccgg ccgccccggc gccgctcgac gcgatgacca gcgcgacgac
    17881 cggtgaccag accatcacca ccacgccgct ggtgctgctt tacgtcgccg cgatgcagga
    17941 ggacccggcc ttcctcgacc aggtgctcaa gagcggcatc gacatcaatt acgtcgatcc
    18001 ggcgtttggg atgaccgcct tccatttcgc cgtgggagag ggccagtttg aggtcgccca
    18061 gcgtctgctg gatcacggcg ccgacatcaa ttcccgctcc gtgcccagcc acgccacgcc
    18121 gttgatcgac gccgtcaatc tcaaccagac cgagacgatc cgctggctgc tcgcgcgcgg
    18181 cgccgacccc aacgtccgcg atgggaccgt cggcgtcacg ccgatgctca gcgcggcgat
    18241 ggacaatcag gtcgatgtct tgcaattgct gctggagcac ggcgccgacc ccaacatgat
    18301 gaacgggcag ggcgtgcccc ccctccacct ggccgtttcg atgggccacc tggaatcggt
    18361 cgagctgctg ctggcgcatg gcgcggaacc caacctgccc acgggcgagg ccggcgtcaa
    18421 gccgttgacc gtcgcgctgc gccacccgga acgccccgaa atcatccgcc tcctgaaaga
    18481 ggcgggggcg acggagtagc cccgaccgct ctttcctcag tgtaacccat cgaatacgca
    18541 taaagcggat ggatgaaaat ccagtccaga aaattgcgca acagcgggta ggtggcgaac
    18601 aggcgatcaa acgtcgagtg atagacgtag tatttctcga cgttgcgcag cccttcggtc
    18661 aggtgttcat aatcgacctc ggccgcgccg gcgacgttgg cgaaacacgc gctgtaccag
    18721 tggatcagcc cccagtgcat aagctgcagg caatggccga agtggagcgt ctcggcctgc
    18781 agcaggtctt tgcgaaacag ccgatggcgg aagtagcgcg tcagcagttc atccagctcg
    18841 ggtcggcgtg ggtcgaaccg gatttcgcgc aggcgccgcc agtcggtcgc cgccggcaac
    18901 gtggggagct ggaccgcgcc gcggccggcg gcgtgccgaa aataggtatt catcaccatc
    18961 acgtagctgc gcagccggcc gcgccgttcg ccatacgtat tgcgcagcgc atgggcgaaa
    19021 cccaggaaca tgcggcgcaa cagcgggctg gcgccgcggc gccgcgccag ccgcaacgga
    19081 atcgcccacg gctcgcccgc ctcaccacgc atccgccggc gaaaggcggg caacaacttg
    19141 tcctgccctt cgcttccggc cgtcatctgg ccggtctgct cgcgcgcctc gcgcagaaac
    19201 cgcaccagca gctgcatata ggccgcctga gccaccagcc gcacgtcgat cggcccgagt
    19261 gccgggtcga gcagatcggc caggtcccgc tcaatcgcct catactgctc ccaggagagc
    19321 gggatgaggt tggtcagacc gggcggctcc tcgatccggt gccggctgaa agtccagcgt
    19381 tgcaggtcgc gcagcgcgtc gccctggtcg gcgacggcgg gccccaggcg gttcagcacg
    19441 gccgtgcagg cgaacgagag gccgacgtag tcgccgcgcg gtgtctccag aaaccgaaac
    19501 gggaagctgc ggcagatgtt gggtttggcc gtttcgccga aggcggcgtg cagcgcgcat
    19561 agaccggcgc gggtgttcag cgtgcacgtg ccgtccgtca tccgcatcac gtgacggccg
    19621 ggcgtccacg ggctttcgac caccggattg ttcggatcgc gcgccgccgc cagcgccgca
    19681 acgggacgcg cgcggatctc ctcggctttc tccgccgaga ccggaatctc ccagaactcg
    19741 ccgcacgacc gcccgcaacg aatgcattcg taactcaggt tgtcgggcag gtgaagctgg
    19801 gagccgccgg tgttggccat ggagaaccct tgcggggtat gaaggcccgc cgaaccgggc
    19861 cgcggaaccg tccgcccgca acggcgaacg gcggaaccag tctatcacaa accggttgcg
    19921 cgaagggagc ggggcacggc atgctcgaag cagcccgaca ccgcgaacgg acgcggacgc
    19981 gagacgcttt tcagggtgag ggacaatggt ttcccacgaa cacggaaacc ttgaatcgaa
    20041 ttaaaatcct gtatgagatc tttttcgtgg gtcttccatg acccttatgc cctttgcgtc
    20101 ccgtatgtcc tttccctgac ggaatggcgg catggcaaaa caacccgcat ggcatcaggc
    20161 tctggcggcg gcgcgcggcg gcgacctgga cccggcgcgg cgcctgcacg ccgagctccc
    20221 cgacgacccg ctgaatgact acttcttcgg ccggtgcctg gccgaggccg gcgtggaact
    20281 ggaccgggcg tccgagctcc tgcgccgcgc cgtgacggcc gaaccgaaga acccgctgtt
    20341 caaccagacg ctggcactcg cgctcatccg ccggcaactg ccggccgacc gcgccgaggc
    20401 catcgacctg ctcacgcgat tcggtctgcc acatgaactc gacctgctcg ggcaactggc
    20461 gctgacactg gaggcccaga cccggccctg gccggcgcat tcccccgcgg ccgaaatccc
    20521 ctggcccaac ggcctcccct cgccccagct cgacccggcc ccgccgcccg atcccacgcc
    20581 ccagcccatc gaccaacccg cggaacagga caatttgtca ccttcgcctg ggacagaagg
    20641 acgccgcaat tcaacgcccc tcgcccgcgg aaaggctccc gcggccaccg atctgaagcg
    20701 cctggaacgg ctcctcgtca gccatcagcc gcttgacgcc ttgcaattgg tcggcgcggt
    20761 cctggaccgg ggacgtgagg acgcctacct gcacctgggt ggcggtgtcg cggctgaact
    20821 ggcgggcgac ccgctccgcg cccgcgccca cctgacccga gcgctggagc tggacccgcg
    20881 ccagatgctg gcgcgcgcct ggctcgggcg gatttactgg cgctcgggct ggtgggatct
    20941 ggctgctgcg gtctggcgca gcctgcccgt ggaagggcct tacgaccacg gccggcacta
    21001 tctcctggcg ctggcgctcg acggcaaaag cgatcgcaag gcggcgctgg cggcgatggc
    21061 gacggccctc aaggagttct tctacgacac gtttcacttc tccatccagg acgcctggcg
    21121 ccgctggcgc gaccaggcgg ccgcgccagg gcagggatga acctcgtgcc cggctgctgg
    21181 tccaaaacct gctttttcaa ggctcgacgg agccgacagc atccggcccg ggccgttggt
    21241 cgcactcccg ccggatgcca tctccgaaac caatgaaatc agcgtcgagt tgtccgttga
    21301 gtcccttcag ctcccttgtg ccccgctcca cgattcaagg cggatcatgc gattgaccat
    21361 gcgccggctt ctctccctca cgctcctctc gctggggctc ccgttcagcg gcctcgcggc
    21421 gccgggcgac gatctggccc gccagggcaa cgaactctat caccaggagc gttatgacga
    21481 ggcagtggcg gcttaccgtc aggccctgga gaaggccccc gactcggcgc ggattcaata
    21541 caatctgggc tcggccctgg cgcaacaggg ccagtatgcc gacgcggaac aggtgctgcg
    21601 tcagacggcg acccgcctgg gcagcggcgt tcagcgcgac gcctactaca acctgggcgt
    21661 aacggttgcg aaaagcgccg atgccctcgg tcagggtggc ggcgggggcg ccggctcctc
    21721 cctgcatcag cagatggccc cgcagatgga gatcgcgcgc ctggaagaag ccctggccgc
    21781 cttccgcgag gcgatgatcg tcgacccgcg cgacgacgcc gcgcggtaca actacgaggt
    21841 cacgctgcgc cggatcgaaa agctgcgcca gcaggcccag caacagcagc aggatcagtc
    21901 cggccggcaa cagagcgacc agcggaacca gcagcagcaa tctggcgaag gcgaccagag
    21961 ccaggacggg caaaacaacc aagacgacac gagccagaac gaccagggcg gccaacagca
    22021 gcaagagcaa caagagcaga gccaggacca gacgccgccg gatcaaccgg agccgcaaca
    22081 gggcgaagcc aacgacgaac agaacgacga aagccaggaa cgccagcagg agggtctgga
    22141 gggcgccgcg ccgacccctc aaccgacgcc gccgagcgag cgccaggccg agcaggctgg
    22201 cgcggcggcg ggcgaggagc ggccacgcga actgacgccg gaacagatgg atgccgaacg
    22261 cctgctcaat ctgttgagtg aggagcagcc cgaacagttc aaacggctgt tccagttccg
    22321 cgggcaaacc cgtccctcgc gccagggaaa ggactggtga tgcgccccct ccgccggctc
    22381 ccgctcctct tcgtcatctg gctggcgctg gccgcgccgc cggccccgcg cgcgcagggc
    22441 gagccgacgc tgcatgccga ggtcaaccgc caggttgtcg gcgtggggca gccgttcacc
    22501 tacacgctgc gcatcgaggc cggcgccgcg gggatcaacg ccaagccgtc catccccgat
    22561 ttcggcgatc tgcgcatcct cggcggcccg agccagagct catcgaccag catcttcaac
    22621 ggccgggtct cgatcagtca gtcctacacc tggacgttgg ccgcgccgcg cgagggcacc
    22681 tacatcatcg gtccctctcg catgcgcggc gccggcggcc tcatcgaatc cgaagcgatc
    22741 acgatcaccg tccacgaaca ggtcgaaccg cgcgtcgtct tgccgcgcgg cctgccgtcc
    22801 gatcagatcc tctcggcccg ctcggccgtc ggcaatacgg aagtcgatca gtatctgaac
    22861 ggccgactct tcctgcgccc ccagatctcc aacacgcggc cctacgtcgg cgagccggtg
    22921 atcgtcacgt acacgctcta caacggcgcg cggatcagcc tgggccagaa cacgcgcgcg
    22981 atcatgcccg acgatttcca gggcgttgtc cacgaggtgc tctacagcca cgatcatctc
    23041 gattaccgac ccgaaatgat cgacgggcag cgtttcgatg tcgcgccgct ctatggcatg
    23101 gcgctcatcc ccacccggcc gggcgtcgtc gaggtgggcg gctattcaat cgagacgcgg
    23161 gtcaatctgc cgcgcggtcg cggcggcttc cgcgatccgt tcgacgatcc gatgctcgat
    23221 tttttcggcg gccgggggga accggtgcag gtgccgacgc ggccgatccg gctggaagtc
    23281 cagccgctgc ccgacgccgg ccagcccgac aatttcaccg gcacggtcgg cgatttcacc
    23341 atcgcggcct cgctcgatcg caccgaggcc aatgaaaacg agctcgtcac gctgagcgtc
    23401 ctgctcgacg ggcgcggcgc catcgagctg gcgtcggccc cccgcttcaa ctccgaagcc
    23461 ttcgagctcg tcggcgaaac ggagagcgtc aacaagctca acaccgccga acagctcggc
    23521 ggcagcaaga ccttcgaatt cgtcctgcgc ccccgcgcga cgggtcggct ggaggttccg
    23581 tcgatccagt atcctatctt cgatccgtat cgcggcgagt accggattct gcgcaccgag
    23641 ccccaggaga tcacgatcgc ggcggcgccc aacgccgcgc aaccccagac tccgcgatcg
    23701 cggaccaatc gggacgaacc gggcgagccg caactgcatt ggatcaagcc gatcacgcgc
    23761 ctggagacaa aaccggcggc gccgctggtt gagtcggcgg ccgtctgggg cgcgcagttg
    23821 agcgccctgg cgctgctcct cgtagcgtgg cgccacgacc gcaggcgcgc gcggcgcgac
    23881 cccgcccgga ttcgccgcga ggccgctccg cgcgagttcg agcggcgggt tcaggcgctg
    23941 cgccgcgaac cgcgcgatgt gcgcgccgag gacctcgcgg gccgactgga gcaggccgtc
    24001 cgccattacg tcgccgatct gcatgggatc tccgccgatg gcttgacccg gcccgagatc
    24061 gagagtcggc tgcacgccca ggggctgtcg gccgaacgca ccgccaaggt catggaactg
    24121 ctcgacgcct gcgccgccgt ccattacgcc cccgccccca gcagccgcga gcaggtgatg
    24181 gcctgggccg acgaactggg cggactgctc cggggaggcg ccgcccgatg agaggactcg
    24241 tgcccaccct ggtcttgctc gtggcggctg cgttcgccat ggcgcaggac gaagacgtcg
    24301 cccgcgcagc cgtcgttccc cagacgcagt cggaaaaccc cttggcggcc accgcgcgcg
    24361 acgtgctcgc ccaggccgaa cacgcgttcc aggcgggcga ttacgagcgc gcggcggaac
    24421 tctatcagtc attgctggag caggccccgc gcgatcccag gctgctttac aatctgggca
    24481 ccgcgcatgc tcgtgcgggg gaaaccggcc ttgccgtctg gcgctacatg caggcgctgc
    24541 agctcgaccc ccgcgacccg gagttgcgcg ccaacctgag gatcctggct cctaacctca
    24601 aggacgacct ggcgatcgcc cccgttcccc cgcttaactg ggcctactgg aagttgaccg
    24661 gcaacgagtg gacgaagctt gcggcgctgt tcaccgtcgt cgctctgctt cttggagcgg
    24721 cggcgttcct gcgcccgcgg atgacgcgcg gacggtatgc cctgcgcctc gtcggttggg
    24781 gcgcggccgt cgccgccctc ctgctctggg ccccggcgct gctgcactat cagcgcgagg
    24841 agctctcctc cgaggcggtc gtcgtcgccc gcgagacctg ggcgcgcacc ggcccgagcg
    24901 aaaatgagat cgaaaccttc ggaccgctgg atcaccgcat cgtgcaagtc atcgatttga
    24961 cgcacgaggg ctgggtcaag atcggcctgg gcggcggccg ggtcggctat gtgcaacgcg
    25021 acgcgatccg gtttatttag ggaaccgaag aaggattcga gacgccacat ccctcttaag
    25081 caatcatcgc catgaccgcg acttgcgcgc cgcggtcatg gcgatgattc gaagactggt
    25141 ggagggagtt ggattcgaac caacgtaggc gtacgccaac gggtttacag cccgtcccct
    25201 ttagccactc gggcatccct ccacacggtg gccgtcgtat tgtagcgtac ggcggcgcgg
    25261 aatcaagcag cggatgatcc cacgacgcga accccaccgg gccgtcaaat cgcgccgcgg
    25321 aactggagct gatggtggga ctcgaaccca caaccgctcg cttacaaggc gagtgctctg
    25381 ccagattgag ctacatcagc ggcaaagccg gttagaacga tcggacatgc gaaaatgccc
    25441 gtaaaaacac tctattatcg cgctaactgg gctcaaatca agctcttttg ccgccgaaat
    25501 ttgcccttga atttgtccgc caaacagggt tttttagagg gcacctgagc cgcaaacggt
    25561 tggggtcctc ggcatgaatt ttcatcggtg cctcctttac ctgcttcatg ccggaccatc
    25621 gcggtcgatc ctgaaataag cggcatcggc cgatgcggtg ttgcggcctt ccgcgtcgac
    25681 ggcgacgcgc cgcgccgcgg gttggggcgg tttctttctc tcgcgctcgg ctccagacct
    25741 ctcgaacccg gacgaggttt cattcctgaa aggattccac gcgctctcat gaccagaacg
    25801 gcctctgctc tgtgggatca ggctctcgac gaattgcgtg agcgtctgga tcgtgagtcc
    25861 ttcacgactt ggatcgaacc ggtgcaactc ctgcgccagg aaggccatgt cgtgcagatc
    25921 ggcgtgccca ccctcttcta tcgcaactgg ctggccaaca atttccatga accgatccag
    25981 gattgcctga gccgtctgct gcgcgccgac gtgcagctcg agtacgagat catgaacgaa
    26041 gtggacgagg aggaagcgcc gctcgcgccg ggcgggttgt cgcagaccga gcagcgggcg
    26101 atggaggtct tccgccgcgg ccggcgcgag cgcgacggcg cccagccgac cgacctgatc
    26161 acctgccccc tcaacgccaa ctacaccttc gagacgttcg tcgtgggcga gtccaaccag
    26221 ttcgcccatg ccgccgccca ggccgtcgcc gatcccgaat cgcgcgccta caacccgctg
    26281 ttcatctacg gcggaacggg actgggcaag acgcacctga tgcaggccat cggccagaag
    26341 ctgcaaacct acggccagca cttcaacgtg ctgtatgttt cctccgaggc cttcatgaac
    26401 gcgttcatcg aagccgtcgc ccagaagaag atggtcgagt tccgcaactg cttccgcaat
    26461 gtcgacctgt tgctcgtcga cgacgtccag ttcttctccg gcgccgagca gacgcagacg
    26521 gagtttttcc ataccttcaa cgtcctctac gacgccggca agaagatcgt gatctcgtcg
    26581 gaccacccgc ccaaggaact ccacgagctg gaggagcggt tgcgttcgcg cttcgaatgg
    26641 ggcctgatcg tcgacatcca gccgcccgac ctggagacgc gcgtggcgat cctgcggcgc
    26701 aaggcgcgaa tgatgaacat cgaactgccc ccggaggtca ccatctacat tgccgaacgg
    26761 atccgctcca atctgcgcaa gctggaaggc tcactcaagc gcctctcggc gcactcgacg
    26821 ctgacgcgcc aaccgatcac gatggagatg gcgcgcacca tgctcggccc gttcctggtc
    26881 ggcgaggagc cgcgcaagat ctccatcgaa aaagtgcaga tggccgtctg caactacttc
    26941 aacatcacgc tgcacgagat gaccggtccc aaccgcgcgc gcaagtttgc catgccgcgc
    27001 cagatcgcgg cctacctctg ccgcgagctg accgaccagt cgttccccga gatcgcccgc
    27061 aagttcggcg gccgcgacca cagctccatc attcactccc accgcaaaat tcagaaggac
    27121 atggccgtcc aactgagcct gcagaacctg atcaaatacc tgaccaagac gatcaaggaa
    27181 gaaccggccg gccattgatc ccccggccct gcaaccagcc ggttccaagc tcttagtttg
    27241 cggatcgcgt actactatct tacaagctac atgcaagtgg gggccgtccg agatgccgac
    27301 ccatgaggag acggttcgat ttctccgcaa gttccgcaaa ttcacaacag atcagcaacg
    27361 tcaattcctg ttgcaggagt cgaaagttgg gaagcatcca agacgaacga ggcgtctttg
    27421 attcgcccaa atcgggctgc cctcaggcca cgccccggac ccgctcggta acccgtaacc
    27481 agtacgcccc gggcaccgct acagccactc ggccctcagc tcctcgtccg aaatctcggt
    27541 gatcgggccg attcgctcca gttcgcgccg gaaatcctcg tccaccttgc cgacgatgag
    27601 caccacgagg cggtccaggt gcaggttcgc cgcggcggct tgccgcacct gttcgctctc
    27661 gacccctttc agccggtcca cgtaagtctc cagataatct tccggatagc cgaactcgtc
    27721 gaggatcatg gcctgacgca cgatctgttc ggacgtatcg aagaaaaaga cgaaggcatt
    27781 gacgtcggac tggatggcga gctgcgtctc ctcggcgctg gggggcgcct gcgtcagcgc
    27841 ctcattgatg tcgagcatca ggcccagcgt ttcgctggtg gcctcgacgc gcgtcatggc
    27901 gacgttgaaa tacagccccg cgaccgtccc ctcgcccagc cgcccgaaca cggaataggc
    27961 cagaccgcgg ttggagcgca cctccttgaa caaacgcgac gtaaaccctc ccgccagaac
    28021 gctgttcagg accttcgccg cgaacttgcg ttcgtcgaag cggccgatcc cctcgtgccc
    28081 gaagtagatc acgttctgcg tcacgtcgcg cgggacgatg taaacccgcg acgactgcac
    28141 gggttcgacc gccggcagat cgattcccgg cgccggctcg gcgggcagtt ggcccagcgt
    28201 cgaccgcatc agccgttcgg cctcctcgcg gtccagatcg ccgatgaccg ccgcccgcag
    28261 attgccggcc gtgtagaagc gctcgtggaa cgcatgcaga tccgccggcg taatggccgc
    28321 gagcgtcgcc tcggtcgccg tccagcccca gggattcccc ttgccgtaga cgagctgcgg
    28381 gaacagcgct tgcgccacat tcatcggcac atcgagccgc tggcgcagcg ccaccgcaaa
    28441 ccggccggcg gcttcctcca gcctgggctg gggaaaggcc ggcgccgtca gcaggtcgcg
    28501 cagcaccccc agcccccatt cggcgtcctc gggcaacacc gacagccgga tccaggtcat
    28561 ctcctcgtcc gactcggcgc tcagctgcgc gccgcgcgat tccagcgcca catcgagcgc
    28621 gtcgccatcg agcgcggccg agccccccgt ccgcagcacc tcggccgtca gactggccag
    28681 accgaccttc cccggcggat cgttcaccga cccggcgcgg atccccacca gcacggtcac
    28741 cagcgggatc gtccgctccg gccgatggta gaacatgatg ccgttctcga aggtcaagtg
    28801 gtggaccggc ggcggcatga actgcacggg ctcgaaggtg agtccgaacc gcgcctcggc
    28861 cgtgccggtt ttcggcgcgg gctcgtccgc ctcctgggcg gagatcaacg ccgcaagcga
    28921 aagcagcagg atcacggaat aacgggcagg tcgaaacagg cgcgccgggc cgggcagcat
    28981 cgtgcatccc tccttcatgg gccgattcgg ttcgctcctg catcatacac gttcgccgcg
    29041 ccggaatcgc gcaagaatcc cactgaagac gcgcttgaac cccaatgacg tttgtcgggc
    29101 gtgggctcgg tcaggcgacg acttccggca cgatccccgc gtggattctc tcgtccggac
    29161 gcgcatgata cgccgtgccg taggccacga cctccaccat gacgttggct ttctcgctgg
    29221 tggtcatgcc gccaatatcg gcggtggaga acctcacgtt gcagacggcg tcgaacccgc
    29281 gggcgcgcgc ttcgctcagc attcgcacga ctgcctcgcg ccgggcgcgc gtcatcatgc
    29341 cgcgaaaact gcggatttcc ccgccgaaga tcagccgcca cttcgaaaga aaggtcttga
    29401 ggtaatcact cgcgatgacc acctgcccca ccaccagcgt cccgccgaca gcgcccggcg
    29461 cgcggcccgg gaagcgccgc aggtcggtgc acagcatcgg ccgcagggct tgctcggcct
    29521 cggccagccg acgcaggtgc cggcgctcgg tcatcgtccc gccgaacagg ccgaccacga
    29581 tcagcacgaa aaaccccagc agcgacagga aggcgaaggc cagctcgatc atcggttcga
    29641 ctccggcccg tggccctcgt accacgcctg gccttcgggc gcgccgccgg cgatcggccc
    29701 ggcctcgggc acggcaatga cggccgtgcc gtaggcgtaa atctccgccg ccccctgcgt
    29761 gacggcgctc gtgctgaagc gcacgttgag gatcgcgttg gcgcccagct gctgggcctg
    29821 cgccatcatc cgggcgacgg cctcctcacg cgcttcggtc aacagttcgg tataaccgcg
    29881 cagctccccg ccgaccaggt tcttcagccc ggcggcaaag tcgcgcccga tgtgtttggc
    29941 gcgcaccgtg ttgccctgca ccagcccgag cacctgctgg atcaccatgc cggggatctc
    30001 ctcggtattg accaggatca tcggatgact gttcacggat cggccctcgc tctcccgcgg
    30061 gattccagct cctccgtatt attcgccgcg gcgggaaaaa gaccagcagg aagaaacctg
    30121 gtcgccaggg aggcccccca tctcttttgt ccctcacctc tatgtccctc acctctatgt
    30181 ccctcacgtc ctctgtcctt tatgtccctt cacgaaccac gaacgacaaa gaacgaaaat
    30241 agccttgacc cccacccgca acgactatat cctgtcatcc ggatataagg atggaaaccg
    30301 cgcccctcag ctccctcttc cgcacgttgg gcgatccgct tcgcctccgc ctgctgcata
    30361 tcctctcggt ggaagagctg acggtttcgg agatcgtccg cgtcctggag cttccgcaat
    30421 cgaccgtcag ccgccacctg aaggccctgc gcgagcgcgg cctcctggcc gaccgtcccg
    30481 tcggttcggc cacgttctat cgcgccagcc tcgccgccga actgggcaac ggcgacacct
    30541 tgctgcgcga tacgctcgcc accatgctcc gcgagacgcc cctccccccc gccgaccgcg
    30601 cacgcctgga gcaagtgctc gctctgcgtg aggcggaggg cggcgaattc ttcgaccgca
    30661 tggcgcaccg ctgggatgcc ctgcgcgagg cctgtttcgg cccgacgttc catttcgagg
    30721 cgttcctcca cctgctgccc gcgccgtggc gcgtggccga cctcggcacc ggcaccggtt
    30781 acctgctgcc ggcactcggg cgacggttcc gcaaggtgat cggcgtcgac cgcagcgctt
    30841 cgatgctcga gctggccgga cgccgcgtgg ccgcaaccgg tctgaccaac atcgagctgc
    30901 gcgtcggcga gctggaaggc aggctgccgc tccgcgcggg tgaaatcgac ctggcggtcg
    30961 cgatgattct gctgcatcac ctgcacgacg tcgcggcggc gctgcgcagc attcaccgcg
    31021 ccctgaagcc cggcggccgg ctcctgctcg tcgagatcct gccgcacgag aatgaagcct
    31081 tccgcgcccg catggccgac cgccgccccg gcatcgaccc cgaccagatg cggcgttggc
    31141 tgaccgaggc cggcatgcgc gaaatcgagc gctgggacta ccccttcgac gcgcatcccg
    31201 agcacgaact ggcgccgctg cccggactct acgggatgac ggcggtgaaa ggctgacgac
    31261 tcccgccagc ctcccacggg gacggcgcaa cccgtccacc ccaatacgta tccagatcga
    31321 atccatctga aaggatgacc cgccgatggc aacgacgatg actgagattc ccccctacaa
    31381 ggtgaaggac ctggcgcttg cccgctgggg ccgcgaggaa atccgcatgg ccgagcacga
    31441 gatgcccggt ctgatggcga cgcggcgcga atacggcccg cgcaagccgt tggccggcgc
    31501 ccgcatcacc ggctcgctgc acatgacgat ccagaccgcc gtcctgatcg agacgctgct
    31561 cgagctgggc gccgaggtcc gctgggccag ctgcaacatc ttttcgacgc aggaccatgc
    31621 ggcggcggcg atcgccgagc gcggcgtgcc cgtcttcgcc tacaagggcg aatcgctcca
    31681 ggaatactgg caatatacca agtgggcact cagcttcgaa cagagcggcc cgacgctgat
    31741 cgtcgacgat ggcggcgacg ccacgctgct gatccaccgt ggcgccgacc gcgaactcga
    31801 attcgagcag accggccgca aaccggccgt ctgcaccgac aacgaggaaa tcgaaatcgt
    31861 cgaccgcctg ctcaacgaca cgctcgacga aaacccgcgc ctgtggcgca cgatggccga
    31921 gggcatcgtc ggcgtcagcg aggagacgac caccggcgtc catcgcctct acgagatgga
    31981 gcgggccggc acgctgcgct tcccggcgat caacgtcaac gactcggtca ccaagagcaa
    32041 gttcgacaac ctctacggct gccgcgagtc gctgatcgac ggcatcaagc gcgcgaccga
    32101 cgtgatgatc gccggcaaga aggccgtcgt gctcggctac ggcgatgtcg gcaagggctg
    32161 cgcgcaggcc ttccacggca tgggcgcgcg cgtctacgtc accgagatcg atccaatctg
    32221 cgcgctgcag gcctgcatgg ccggcttcga ggtcgtcacg atggacgaag cctgcaagtg
    32281 gggcgacatc ttcgtgacga cgaccggcaa cgtcggcgtc atctcgcgcc ggcacatgga
    32341 ccagatgaag cacgaggcaa tcgtctgcaa catcggccac ttcgacagcg aaatcgacgt
    32401 cgcctcgctg acccgcgaca agagcctcaa gtggcacgag atcaagccgc aggtcgacca
    32461 gatcgaatgg cccgacggca agcgcatcac gctgctggcc aagggccggc tggtgaacct
    32521 gggctgcgcg acgggccacc ccagcttcgt catgagcaac agcttcacca accagacgat
    32581 cgcgcagatc gagctgtgga ctgagcgcgc gagcggcaag tacgggaaga aggtctacgt
    32641 cctgccgaaa cagctcgatg agaaagtcgc gctgctgcac ctggatcagc tcggggccaa
    32701 gctgacccgg ctgaccgaac agcaggccaa atacatcggc gtcccggtcg acggcccgtt
    32761 caagcccgat cattaccggt attaattcac cttaattgaa ggcaatgaag gtccgccggc
    32821 acaacggata ccggcggact ttttttgaca gtcgttgggc aggcgcgaga gctcggcttt
    32881 cgcttcgagt ggcaaatctc gtcgcacacg aagtgatcga tgagcagaaa aggtaaactc
    32941 caggtccttt cgcgtaaatc ctggtcgtcg tgcgatgcgt gagagcccag ccagcggcca
    33001 tcatcctcct gctcgtattc gatggtgaat gtcatccggt tcttccttgt cacgatctcc
    33061 agcgcgggac gagactccat tccgtgactt cactatagat tggcgccacg gagccgcaat
    33121 ccaatgaggg gtagcgcgct ctcctgatct ttacaggcac agccgtgcgc gacacctgcc
    33181 tcatgattag cgcccgatac gctcgaccga tcttctccgc aaacgcctcg cgactaatac
    33241 ctgaaaaaca ggatcggatg cgccagcgcc ttcatgatcg cgcacgtcgt gctgccggcg
    33301 atcagggtct tgacccgcga gtcgccgaac gcccccatca gtaccagcgc gtcggggtat
    33361 tgcgccaggt gctggatgat ctcgtcggcc ggctcgaagc gggcggggat ggcatcgaac
    33421 gtgaactcaa gctgatgatc ggccaggtag cggcgcgccc gctcgttgat ccactgcgcg
    33481 tcggcgtcgt cgtgcgccgc ggtcacgatg cgcagcggca gcccggccag ccccgccagc
    33541 cgggccccga acgccagcgc gtgcagcgcc cgctcgtccc cgttatccag aatgatgatg
    33601 tcgcgcggcg gatgataggc ctcggcggcg acgagcaccg gctgcggcgc cgagcgcacg
    33661 atcgcccgcg ccagccggct cagccggcgc cgctccggat cctccggctc gggcgatccc
    33721 ttgccgatcg ccagcaggtc caccatcgcg ccccgcgcca ggatctcttc ctccgcatac
    33781 cccgtccgca ccattgtctc gaacggcacc ggccactcct ggcagatcgc ctgcacgcgc
    33841 tcgaacagcc tctgcgcgtg ggccagccgc tcctgccgtt cggcttcgat ctcctcgggc
    33901 gaacgcgacg gcggcatcgc cgccagatat tccatcgaag ccgtcatcat ccccgtcgtg
    33961 acggccgggt cgttcgtcaa ctcaaccaca tatccggcat aaatcttccc ctcgaagagc
    34021 gaagccagat ggcaggcgta gcgcagggcc gcctcggccg atggcgatcc atcgtatgcg
    34081 acgaagacgc tcttcatctt ccgccctcac ccccgcctgc ggatcatggt gtcagtatac
    34141 atcaacctgc ggcaaacggg aaccagttga caaccttttc tccgggtgcc gatcatagcc
    34201 gatgattacg cgcgccgcaa ctcgcggccg acccgcgcgg ctggaatcgc cacggagaga
    34261 tcatgcgccg tttcatccca cgatcacccg caaggcgttg cccgccggcg ctgcacatcg
    34321 cgctggcgct cttcgccctg ctggccctgc ccggcgttcc gcgggcccaa cccaccgctc
    34381 ccggcacgac ctacgagttg ctcggcgaac gcctccagcg ccggttcgaa atgatggatt
    34441 tcggcggggg ccggctcgcc gcgctcgtca ccgacgcgcg cctgccgatc gtcgcctatg
    34501 cccgcgatga acaccagccg ctcgcggcgg gcagcttgat ccagattctg acggcggccg
    34561 ccgccctcga ttggctcggc ccggaatttc agttcaaaac tgaactgcgc atgactggaa
    34621 accgcgcggg acggacactc aacggcgacc tgatcgtcat cggcgacggc gatcccacca
    34681 tcggttccgt ccaccgcagg ccgccgccga agcgcaaacg gcccggtttt ctgctgctgc
    34741 ggggcgcggc gcggctcgtg ccgaacgagg cggccgcctg cgagctcttc gatgactgga
    34801 cggctcaact caggcagcgc ggcatccgcc gggtcgaggg ccgcgtcatc ggcgacgcgg
    34861 cgcggttcga cggcgagggc gcggcgcccg gctggccgat gcggcagcgc ggctcggccc
    34921 gtctgccgac gatcagcgcc ctcaacttca atgacaactg cctcgacttc ttctggcatg
    34981 cgagcaaccg catcggcatg ctcgccgatt tctcgctctt cccgcggctg ccgggctaca
    35041 accacgtgtc gaacaatgtc cgcgtccaga tggtctcgcc cgccgagcgg cgctatgaac
    35101 gggtcgaggt gggcaatctg atcgcggcga tcggctcgtt gtcgcccgca tccgtggcgc
    35161 acgaccgcgc ggcgatcgcc tcgccgcccg aattctttgc ggtcgccctg cagcggcgca
    35221 tggaggcccg ggggatcgcg ttcggcggcg aacccgccgc ggcgggcaac ctttcgcccg
    35281 gcgaactgtc ccccgccggg gaactgctcg ccacgcacct ctcccctccc ctgcccgaga
    35341 tcctcgccga catgctgcgg cacgacgccg cgcttcatgc cgaatgcatc ctcaagacgg
    35401 ttggcctggc cgaatctcag gccgagaccg gaggatcgtt cgccgccggc gtccgcgccg
    35461 tgcagacgat gctgcgccgc cagcgcgtcg gcggctcgcc gcccacgctc ctcgatggct
    35521 cgggacgctc cacgctcaat cgcgtcagcc ccgcccatct ggtccgcacc atgcaggcga
    35581 tgctgaccgg cccgcagggc caggtcttcg aacgcctgct gccgcgcgcc ggcgacgcgg
    35641 gcgtcctcga gggccacttc cagggccggg gcgatgccgc ggcggccgag ccggtcgacg
    35701 cgaaaccgcc cgtttgggca atccccgccg cgggagaagg ctatcaggcg ctggcgggct
    35761 gggccgaggc gcgcggcggc cggcgcttcc tcttcgcctt catggtggaa ggttcggctc
    35821 tgcccgcgac ggtcttgcgc gacaacctgg accggctggt tctggagatc acgcgcctgc
    35881 ccacgcagcc gtgagtccgg ccaacgccgc ccccagccgg ccgccacgcg ccggtttccg
    35941 cggccgggcg cgccgaacgc tctactggct cctgcccgtc gccgtctatc tgggctgcgc
    36001 tctgcatctg ccgcgcaatc agggccggga tctggtgccc acgcccgacg ccgtcgagta
    36061 cgcgatgctc tcgcagcgtc tggcgcgcct ccagccgccg ctcatcgaca ccgggcttca
    36121 gctttatccc tcccgctatc cgctcgcgtt tccgctggcc ctcgcgccat tcaactggct
    36181 ggcggggttc gacctgaccc gcctgcactg ggcggcgacc tgctacggcc tgctctccgt
    36241 gctgctgatg gcgcgggtcg gcgcgtggct gctcggttcg cggaccgcgg gcggcctggc
    36301 ggccctcttc tgggcgctgc atccggccct gctcgaagct tccgcggtcg tcatgagcga
    36361 gacggcgatc gtcgccgcat ggctggcgct gctgcaggtg gggcggccgt ggcttgaacc
    36421 cggcggaccc gcgcccggcc tcgcgcgcgg catcgccgcc ggcctgctgg ccggctggtt
    36481 gaccgtggcc aaggcgccgt tcgcctactg ggccctggtt ttcgcgattc tggtctggga
    36541 tcgcgcgctg gcccgccgcc gcttcgccgc ggcgatcggg ctcaccttgg cgggcctgct
    36601 ctgcgccttc ggcgatgcgc tctatcgtca ctgggccttc ggcgactggc gcctcaacgg
    36661 ctatcagttc tggcacccgg tgatctattc ggagtttcgg cgcacgttca acccgcgcta
    36721 cgcgctggag ggctacgtcg gttacggcgg cggcatcggc aacctggtct actacgcgcg
    36781 ccatttcctg gggctgaccg gcgcctacta cggccgctat gccgcgccgg tcgccgccgc
    36841 ggccttggcg tgcctgctct ggccgcgacg ccgcggacgg ccctggggcc gcgtcgtcct
    36901 catcctggcc ggctgggcgg ccgtgggcgc cctgtttctc gggacgtatt tcttcctcga
    36961 atatcggctc ggcctgttgt ggctcccgct catcgacggc ctcgcggcct ggggcctgat
    37021 gcgcgcctgg acctggagcc gcctgcgccg cggccccttc ctgcgctggc gcgcccatcg
    37081 ctgggcgcag gtgctggcga tcttcgccgc cgcgatcctc atccggagcg ccgcccgcca
    37141 tgcctatccg gtcatgcatc cgccgcccgg ccaggtgcgc gcctcgcagg tcgacagcgc
    37201 ccgccgcctg ctggcgcacg tgccggccga cgcctggctc ttctcgggct gggaggccgg
    37261 cgtcgtgaat cgaatccgac cccggccggg ccctaccgcg ggtctctata gctggatcgt
    37321 cgacgccccg gtgatgaacg gccacctggc caaggctttg gaatttgatc tccgtccggt
    37381 gggcatcacg ccgctggggc ccgccggccg agaagagctc cccgccgcgt ggcggcgcga
    37441 gccggccgtg ctgctcatcg cggcgggcgc cggcgacgcc gcccactgga cgatctcgcc
    37501 cgccgagcgc gagcgactgt ttcaccggcc ggcctacatc cttgcgcagg cgcccccctg
    37561 gttcccctcc accgccgagt ttctgaacaa ggtcgttcta cccacgctgg cgcgagagct
    37621 gcacctggag gaagtggccc ggcacggaga cttcgtctta taccgcgcgc gcgccgaata
    37681 ggaagaacca tgcggaaggt atgaattcga taccgattca tgaaaccggc ggatcacaac
    37741 gagatgggga gtgctcggcc gattcgaacg acccggtcct tgccggatcc ttcaacaggg
    37801 cagtcccgtc agggacggca gacttcagcc cgctagttca ggattccctc ttcaatgctg
    37861 gcggttccaa ttagctaacc cggtaagtcc gcgaagcgga cgacggggaa taaagcgcag
    37921 gatgacggcc gcaggccgga accctgcgtc gccgcccctg acgattcaag cccctggcag
    37981 gggcggccgg agccggtttg aatttgcgga atggcaagtg cttcagtggc gggaaaccgg
    38041 gatacccaaa gcgacaacgt cccgttggga cgggagcacc gatccaaccg atcctgaaaa
    38101 tgaaggacat attttcgagt gagcctgtat gaccggcccg gtcaccgact caagccgaac
    38161 gcctccagca gatgccgcgc cgccagcgtg gggctcagcg tcccgtgcag caccgcctgt
    38221 tcgatcgccg gcaaacgcga ttgcaccgat gggtggcgac gcagcgcgat ccccaactga
    38281 tcgttcacca tgtcccacat ccactggatc cgctggcgtc ggcgccgccc ctcaaactcg
    38341 ccggcggccg tcagcttgcg ccggtgttcc ctgatcagct cccagatctc ttccagtccc
    38401 tgccgcttca gcccgctgca cgtgaccacc ggcggcgtcc agtgcgggct cagcggccgc
    38461 atcagatgaa acgcgcgccg atactccaac gcagcctggc gcgccttgag ctggttgtcg
    38521 ccgtcggcct tgttgaccgc cagcatgtcg gccagctcca gcacgccgcg cttgatcccc
    38581 tggagttgat cgcccgcccc ggcgatcatc agcacgaaaa agaaatcgac catgtcgtag
    38641 accaccgtct cggactggcc gaccccgacc gtctccacca ggatcacgtc gaatccggcc
    38701 gcctcgcaca gcagcatcgt ctcgcgcgtc ttgcgcgtga cgccccccag cgtcccggcg
    38761 ctgggcgagg ggcggatgaa cgcgcgcgga tcggcggcca gctccaccat ccgcgtcttg
    38821 tcccccagaa tgctcccccc cgtcacgctg ctcgtcggat cgatcgccag cacggcgacg
    38881 cggtgcccct tgcccgtcag catcccgccg aacgattcga tgaacgtgct cttgccggcg
    38941 ccgggaaccc ccgtgatccc gatccgatgg gaattgccgg tgtcggggag gatcgcggag
    39001 agcagttcct gcgccaggta ctggtggtcc tggcgactgc tctcgacgag cgtgatcgcg
    39061 cggcccagga tcgcgcggtc gccccggcgg atcccgtcgc ggtaatcttc cagactcagc
    39121 gtgcgccgaa cggggggacg ggaaggggca gaatcaggca tgaagatcga ctgacaattg
    39181 aattgtaagg gaaccgaaca ttgacctcaa atcccgttcg ccctccctga ataatccgcc
    39241 tctcgtcctt acttgaaaat acgtcctcgt cctcgctccc gaaggcctgc ccaatcttta
    39301 tcacctcgtt gtgaaccgca ggctcatgag cgactcgcaa tcgcccccga aaggcccgcc
    39361 cgaaattccg ctctcggctc tcgtccctcg tcctcggctc tcgaaaaccg gccgctttct
    39421 ccttcccggc cgccactgac tcacctgcgt ctgggggtgc gcagcggaac ctggcgacgt
    39481 gcacgcgcca accccgcgtc cccgcagggg gcgaacccaa gccggcaaat ggcgttgccg
    39541 cgcgcctcgt cacctcgcta cttggcgggc agcgcgaaac ccaggtggcc gctcaatttt
    39601 tccagcaact ccacggcggc ctcgccgatc accgtccccg gcccgaagat cgccgccgcg
    39661 cccgcctgat acagcgcctc gtaatcctgc ggcggcacga cgccgccgac gacgatcatg
    39721 atgtcctcgc gcccctgttt ggccagttcc gcgcgcagtt gcggcaccag cgtcaggtgc
    39781 cccgccgcca gcgaactgac cgcgacgata tgcacgtcgt tctccacggc ctggcgcgcc
    39841 gtctcctccg gcgtctggaa cagcggaccg acgtccacgt cgaagccaag gtccgcaaag
    39901 gccgtcgaaa ccaccttctg gccgcggtcg tggccgtcct ggcccatctt ggcgatcagg
    39961 atccgtggac ggcgaccctc ctgctcgatg aacgagctga ccagttgccg gatgcgactc
    40021 accgtccctt cctcctcgcc cagctcgccg ctgtagacgc cctggatgga ctggatcgca
    40081 gcctgatggc ggccgtaaac tttctccagc gcgctgctga tctcaccgac ggtcgcgcgg
    40141 gcgcgcgccg cctgcaccgc cagctccagc aggttgccgg tcccctcggc cgccgcctgc
    40201 gtcagcgcgt cgagcgcgtg ctccacggcg gcctgattgc gctcggcgcg cagcttcttc
    40261 aaccgcgcga tctgcgcttc ccgcacggct gagttctcca ccttcaaaat ttctatccgc
    40321 tcctcctggt ccaggcggaa cttgttgacc ccgacgatcg tctggcgccc cgaatcaatc
    40381 cgcgcctgcg tgcgcgccgc cgcctcttcg atgcgcatct tcggcacgcc ggcgttgatc
    40441 gccttggcca tcccgcccag ttcctcgatc tcctgaatgt ggctccaggc gcgcgccgcc
    40501 agatcggccg tcagcttctc cacgtaatag ctgccgcccc acgggtcgat cacgctggtc
    40561 gtcccgcttt cctgctgcag gaagagctgc gtgttgcggg cgatgcgcgc gctgaaatcg
    40621 gtcggcagcg ccagcgcttc gtccagcgag ttggtgtgca gcgactgcgt atggccctgc
    40681 gtcgcggcca tcgcttcgat gcacgtgcgg atcacgttgt tgaagacgtc ctgcgccgtc
    40741 aggctccagc ccgaggtctg gctgtgcgta cgcagcgaca ttgacttctc gttgttcgga
    40801 ttgaactgct tcatcagccg cgcccacagc agccgcgccg cccgcagctt ggccacctcc
    40861 atgaagaagt tcatcccgat cgcccagaag aacgagagtc gcggcgcgaa cgcatcgacg
    40921 tccagcccgg ccttgattcc cgtccgcaca tactccaccc cgtcggccag cgtgtaggcc
    40981 agctccagat ccgccgtcgc ccccgcctcc tgcatgtgat agccggagat cgaaatgctg
    41041 ttgaaccgcg gcatatgctg cgaggtgaac gcaaagatgt cggcgatgat gcgcatcgag
    41101 ggctcgggcg gataaatgta ggtgttgcgc accatgaatt ccttgaggat gtcgttctgg
    41161 atcgtcccgg agagctgctt gagcgccacc ccctgctcct cggcggcgac gatatagagc
    41221 gccatcaccg gcagcaccgc cccgttcatc gtcatcgaga ccgacatctg gtccagcggg
    41281 atcccgtcga acagaatgcg catgtcctcg atcgaatcga cggcgacccc cgccatcccg
    41341 acatccccca tcacgcgcgg gtggtccgag tcgtaaccgc gatgcgtggc cagatcgaac
    41401 gcgatcgaca gccccttctg ccccgccagc agattgcgcc ggtagaaggc gttgctctct
    41461 tcggcggtcg aaaaccccgc atactgccgc accgtccacg gccgcatgac atacatcgtg
    41521 gcgtagggcc cgcgcaggaa cggcggaaag cccggcatcg tatccagatg cgtcaccccg
    41581 tccaggtcgg acgaggtgta gagcggcttg acgtcgatct gctcgggcgt tgtccaggtc
    41641 acatcctcca gccgccgccc ggcgagctgt tccgcctcgc gttgccagcg cgccagatcc
    41701 cctccgccgg tcggctcgct caactctacc ttggtgaaat ccggaagcgg gctcattcga
    41761 cttcaactcc ttgttccttg agcagcgacc gcagcgtgcc cagaacgtcg caaccgatgt
    41821 gaatgaacgt atcgatgccg gcttcgcgcc agcttgcctc gttttcgccg ggccggccgg
    41881 ccagcacgat ccgccgcgcc ccgcgccgtt tcagctcgcc ggcgacgggc gccgccaact
    41941 gctgatagac ggcgtcggac gaacagatga cggcaaggcc ggcgccgctc gccgcaaacg
    42001 ccgccccggc cgccgcggcg tcgtcgcagc cctcgccgtc gatcacctcg aacccgccgg
    42061 cctccaggaa attgcgggcg tagcccgcgc gcgcggtatg ctgcgccacc gggcccaggt
    42121 tgaccaggaa cgccgacggc cggtggccgg cgcgcgcggc atactcgtcg ctggcggcgc
    42181 gcagcgcttc atacggctcg ctgaacgtgt gaaacaccag cgcgtcgatc gtcaccgctt
    42241 cgtgcccgcc gtcgagcgac tgggccagtt caccgatcgt cgccccggcc tgcgcatcag
    42301 cgatgatcgt ctccatcaac tcgcccggtt ctccctcgcg cacacgcacc tgggcctcga
    42361 gcgcctgcag cgcccggccg acattgccac gccggcgcag cccctccagc cgctggcgcg
    42421 cttcctcccg cagcgccgcg cggtccggct cgcgcggttc cagcgagcgc tcgcccagat
    42481 tgggaaattc gctcacgccg gtgatcgcgt ccttgcgccg cgccaggttc ttctcccgcg
    42541 gttgatacgc cgcttcaatt tgctccttca cccagcccga gacaatggcc tcacccatcc
    42601 cgccccgtgc ttcaatctcc tggaacagct tccacgcggc ggccgccaga tcctcggtca
    42661 gccgctcgat gtaccacgag ccgccgcccg gatccatcac ctgccccaga tgcgattcct
    42721 ccatcaggat cacctgggtg ttgcgcgcga tccgacggct gaagtcatcg ggcaggccca
    42781 cctggctgtc gaacggggta atcgtgatgc tttcggcgcc cgccgccgcc ccggcgaaac
    42841 agcagaccgt cgtccgcagc atgttgaccc acgggtcgcg cacggtcatg atccggtcgg
    42901 cgttgcgcac gtgcagcgcc attgcccggt cccgcggttc ggcgccgcag gcctcggcca
    42961 cccgcgccca cagccgccgc gccgcccgca gcttggcgat cgcaagaaac tgattgcagc
    43021 ccacgctgta cgaaaacagc agttgattca gcgccgccgg caacgtcagg ccgaagccca
    43081 gcatcgcgcg cagatacgcc acgccggtcg ccatcgagaa ggccaggtcc tgaacggccg
    43141 tcgcccctgc ccggtgataa cacgacgtgc cgacgcagac gctgcggctc gacggcatgt
    43201 gacgcgaggt ccacgccgcc agctcggcca gacggtccag cgcttcgtcc aggcgcgtgg
    43261 gcaggcggcc ctcctccgcc agcgccgcca gcggatcggc gttgaaggcg acccgcgccc
    43321 gcgcggggtc cagcccccgc tcgcgcagga gggcgacgag caacccggcc gccggcagaa
    43381 actgcgcccc ggcatccagg ccgattcccg cgatctccag cagcacctga tccagtgccc
    43441 ggcccaggtc ggcgctggag taaatcatca cgccatcctg gccggccagg tccccggcgg
    43501 cggggtcgtc gccgtccaat ccggcgcgcg ccgccgcatc cagcctgaac tgaaccgacg
    43561 tcgccccgcg ctccagatcg gccagcacgg cctgattggt cacggcgagc gagggatggc
    43621 ggtgctcctg gcggatgtcc caccccgcga cgtccagcgc gagcggacgg cgattgaccg
    43681 ggcgcgcacc gcgcgtgaac ggcggctgac ccggcaagcc ggaccggtcg ccctcgaccg
    43741 gccagtcccg gcgcgtataa atcggctgaa tcgacacgcc ttcatacgtc tgcgtgacca
    43801 gctttttctc gaacgaggcg ccggcgaggt cctgttcgac cacgcggcgc catgcctcat
    43861 aactgaccgg tggaaattgc tccgtaaggc ttggattgga caccgtggaa ctcctgccag
    43921 ataatcacca actcggaccc tagcgtcccg aacaccattc tagcgtccgc atccggggag
    43981 tcaaacccgt ttcgtccccg tctgttgtca gtctctcgtc tctcgtcttc ctttctcctc
    44041 ccccgtcctc gtcttcgtgc tcgtccccaa cggcacaacc aagaatttgg cccccggctc
    44101 ccctcgtcct cggctctcaa aaacccgatg tctgccaact cgcgatgaac cgccgaccca
    44161 ggcaccgctt ccaaagagcg taacattccg ctccgccgtc ttccacaatg cctcattatg
    44221 ctacacaacg tgacgctccc aggctccgcg catctcttac aaaccattta ttttcaacat
    44281 attacaaatt gcgtcccgct ggcctttctt ttgctctccc accggtctag attgggatgc
    44341 agggagctgt tgcttagatc atggacatga acaaactgac tcagaagact caggaagcgc
    44401 tcgccgccgc gcagaccctg gccatcgaac acggccatca ggccgtcgat gtcgaacacc
    44461 tgctcgccgc gctgctcgac cagcccgaag gcatcatccc acgcattctg gagcgcgccg
    44521 actgcgacgt cgacatcatc cgaaaaaacc tcgacgcgga actggaccgt atgccgcgcg
    44581 tcagcggccc cggcgtcgaa ccgggccagg tctacgtgac gcaggacctc aaccgcgtcc
    44641 tcgtccgcgc cgaggaggaa gcccgccgcc tgcgcgacga ctacatcagc gtcgagcacg
    44701 tcctgctggc gatgctcgat tcagccaagg cgcgtccggc cggccgcgtc ctgcagaaat
    44761 ccggcgtcaa acatgacgcg gtcctgcgcg ccatggccga ggtccgcggc aaccagcgcg
    44821 tcaccagcaa caaccccgaa gcaacctatg aggcattgac caagtacggc cgcgatctgg
    44881 tcgaggaggc gcgccgcggc aagctcgacc ccgtcatcgg ccgcgacgag gagatccgcc
    44941 gcgccatccg catcctctcg cgccgcacca agaacaaccc ggtgctgatc ggcgagcccg
    45001 gcgtcggcaa gaccgccatc gtcgagggcc tcgcccaccg catcgtgcgc aacgacgtcc
    45061 ccgacggcct gcgcaacaag accgtctttt cgctcgacat gggcgcgctg ctggccggcg
    45121 ccaaattccg cggcgagttc gaggaacggc tcaaggccgt cctcgccgag gtccagaagg
    45181 ccgagggccg catcatcctg ttcatcgatg agctccatac gattgtcggc gccggcaagg
    45241 ccgagggcgc catcgacgcc ggcaacatcc tcaagccgat gctggcgcgc ggcgaactgc
    45301 actgcatcgg cgccaccacc ctcgatgaat accgcaaata catcgagaag gaccccgccc
    45361 tggaacgccg cttccagccg gtcctgatcg aggagccctc ggtcgaggat acgatctcga
    45421 tcctgcgtgg cctgcgcgaa cgcttcgagg tccaccacgg cgtccggatt caggacagcg
    45481 cactggtctc ggccgcgatc ctctcgcatc gctacatctc ggaccgcttc ctgcccgaca
    45541 aggcgatcga tctgatcgac gaggcgggcg cgatgctgcg caccgagatc gactcgatgc
    45601 ccagcgaact ggatcaggtc agccgccgcg tcatgcagct cgaaatcgag caggcggcgc
    45661 tgcgcaagga aaaggacgaa gcctcgcgcg agcggctggc cgcgctcgaa aaggagctgg
    45721 ccgagctgcg cgacgaacag tcgcgactca atgcccagtg gctggccgag aaggagtcga
    45781 tcggcaaact gcgcgaactg cgcgagcaga tcgaacagac gcgcgccgcc gtcgagcgcg
    45841 ccgaacgcgc ctatgacctg aacaaggcgg ccgagctgaa atacggcaag ctgcgcgacc
    45901 tcgaacagca acttgccgcg ctcgaaaaac agcagcacga caagctcggc gccggcacgc
    45961 tcctgcgcga ggaagtgacc gaggaggaaa tcgccgaaat tgtcgcccgc tggaccggca
    46021 tccccgtgac ccgcctgatg gaaggcgagc gcgaaaaact gctcaagctc gatgaaatcc
    46081 tgcaccggcg cgtcatcggc caggatgagg ccgtgcgcgc cgttgccgac gccgtcctgc
    46141 gcggccgctc cggcctgaag gatccgcgcc ggccgattgg ctcgttcatc ttcctcggcc
    46201 cgaccggcgt cggcaaaacc gagctggcca aggccctggc ccaggcgctg ttcgacaccg
    46261 aggagaacct ggtccgcatc gacatgtcgg aatacatgga gaagcactcg gtttcgcgcc
    46321 tgatcggcgc gcc
//
LOCUS       DAOPCO010000005        46032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_929_length_46032_cov_5.284969, whole genome shotgun sequence.
ACCESSION   DAOPCO010000005 DAOPCO010000000
VERSION     DAOPCO010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 46032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46032
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_929_length_46032_cov_5.284969"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PLS90_00920"
     CDS             <1..1445
                     /locus_tag="PLS90_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPK00992.1"
                     /translation="AAFRICRGTSCFLSRGSETIHIHSSHALAHAIACAGYCDRAPAF
                     LDPDGEVHTASRSRPRHALPDLRVMGREPLLLARLLRGDHARLDQARAAGVYAALARA
                     LERPPAEVLRAVEESGIRGRGGAGFPTGTKWRACAEAPGPLRWVVANGDEGDPGSFID
                     RVLLEHDPHAVLEGMLLCGYAVGARRGIVYIRSEYPSAREVMRRAVADARAAGLIGRD
                     VLGSGFTFEIDVISGHGSYVCGEETAMLNAIEGRRGEVRLRPPFPAQTGLFGCPTVIN
                     NVETLANIPVIVERGADAYRRFGTPQSAGTKTICLNNAFARPGIVEVEFGVSLAEVID
                     EAGGGRNGDALEMILMGGPMGSLLPADEWDVPICYGAMRERGLKLGHGGLVGIPRGTD
                     ARRLLEHLLEFMVAESCGKCVPCRLGSRRGRTLVAQLGANGESRAEFTRLLRVIEAGS
                     LCAFGQHMVDPMRRLVELKGDEIFAGACAR"
     gene            1442..4243
                     /gene="fdhF"
                     /locus_tag="PLS90_00925"
     CDS             1442..4243
                     /gene="fdhF"
                     /locus_tag="PLS90_00925"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPK00993.1"
                     /translation="MSEATASIDGRTVAVRDGQTILQAARQAGIQIPTLCYLEGLRPE
                     GGCRVCLVQLDGSPRPVAACHTPLRPGMRVATRNEELFALRRDILSLYLQDGKLNAYH
                     RDHRDSEFTRLLEDHGLAAPRLDPARAGARLDQSHPYMRFQPALCINCRRCVAACDEV
                     QGQFVYSMAERGPFTHLIYGRDGHFLHSPCVACGACVDRCPTGAISDRDRLRGEAPER
                     VVESVCGYCGVGCRIAVAARDSRVLSITGVAAAAVNRGHLCVKGRYAHAYHHSPERLT
                     QPLLRTDDGFKPIRWEEALRVLVDRLRGVRERHGAQALGVFTSSRSTNEATYLLQKLF
                     RTVIGTNNVDCCARVCHSSTALALQQVTGTGAATAAYEDIDRCGCILLAGANPTEAHP
                     VVGARLKQAVLRGARLIVADPRRIELADYADVHLQLKPGANVALFNALAWVLFDEGLV
                     DAAYAAERLEGFAELQQFLARHALTDFTSTAGVEPQQVRAAARILGAGGPVLFVHGLG
                     LSELTQGTASVKALCNLGMLTGSIGRPGGGMLPLRGQNNVQGSADMGSMPNQLTGYQP
                     VGDPEVRRHVESIWGELPPAEPGLTIPEMLTAAVHGRIRALWIQGEDIAQSDPFQQHV
                     RNALSKLEFLVVQELFMTETARYAHLVLPAAGALEQDGTFTNGERRIQRVRAAVPPPG
                     EARPDWLVARDVARAFGRDWDYPHPGAVMDEIARVAPRLFGGVRYDRLGADGLQWPCP
                     SVDHPGTPQVHQAAFLRGRGQLSVTEPLANPEHDVAGFPYTLITGRVLNHYNVGTMTR
                     RTPQSELTDADRLEIHPDDAAREALKDNDRVRICSTWGCTHVTVRLSPRVSPGTFFLP
                     FHFPETATNQLIGPHADPDSKCPQYKVTAVRLSPPVEEPRGEIAAEAVAAPDRPSNGD
                     AHPGPAAREAERNLAGART"
     gene            complement(4393..4818)
                     /gene="raiA"
                     /locus_tag="PLS90_00930"
     CDS             complement(4393..4818)
                     /gene="raiA"
                     /locus_tag="PLS90_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPK00994.1"
                     /translation="MPLQLTGRHMNISRTQKAYIDKKVNRLRRLCPKIDEMHFTLTRE
                     KLHYKADVTFRAGAIAAQANSTASQPLEAIDLLVDKLEAQITKTKARWGNKKALGRER
                     QRAGEAAVLEETLATDAALDDEDEEEALEDEEEPQRVYA"
     gene            complement(5005..5775)
                     /gene="lptB"
                     /locus_tag="PLS90_00935"
     CDS             complement(5005..5775)
                     /gene="lptB"
                     /locus_tag="PLS90_00935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPK00995.1"
                     /translation="MVTRHRLVTHNLVKAYGGRRVVDHVSIPLEQGEIVGLLGPNGAG
                     KTTTFNMVVGIVKPDEGTITLDGKDLSRMPLYKRARAGLGYLTQETSIFRKLTVEENL
                     MSILEMTKLSRQQQRARLELLFDELSIGHLAKNYAYTLSGGERRRVEIARALAIEPII
                     MLLDEPFSGVDPKSVEELQEIIQQMRDRGLGILITDHSVRETLSVTDRSYIIHEGKIL
                     VQGAAEELVNNPEVRRLYLGERFYMEVPGQAREPAGKA"
     gene            6199..7236
                     /locus_tag="PLS90_00940"
     CDS             6199..7236
                     /locus_tag="PLS90_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK00996.1"
                     /translation="MMRNMDSWARDVRTQTERQAMPIMTYPGLPLVGLNVMDIVTSGE
                     QQARCIAALAGAYRSLAALTLMDLSVEAEAFGCPIQYSADEVPTVTGAILHDLEQVEA
                     LPLPSPETGRTAAYLEAARRAAALIENRPLFGGMIGPYSLAVRLRDMSRLMLDLMLQP
                     ERVHALLERVTRFLIAYARGFRAAGAHGLVIAEPAAGLLSPAHCDEFSSAYVRRIVAA
                     VQDEEFLVVLHNCGRTRELVPSLVSTGAGMLHFGNAVDMLDILPQVPRDVLAAGNIDP
                     SSVFRHGKPEQVRSATLALLRRTADYPNFVLSSGCDVPPGTPLQNIDAFYGALDEFNG
                     RRGEATLADIG"
     gene            7343..8299
                     /locus_tag="PLS90_00945"
     CDS             7343..8299
                     /locus_tag="PLS90_00945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00997.1"
                     /translation="MLLSVIINTYERPESLSRCLQALTRQKEADPFEVVVVDDGGRLD
                     LKAIERVYQDKLDLRLRRIEHAGRAAARNAGIRWARGERILFLGDDVIVRPGCLARHR
                     LERDPAVARVGPFPLAEPPRSPALARWAEPNPQHLIADPTNAGFLFCSTGNLSIDRER
                     IVALGGFDERFARYGWEDIDLGLRFEREGGRIVFDPEARAVHDHRWRGRAALWRRERE
                     AGYTAFQFWSKWQALAPGAIRSMKFWDDAAALRPGPPWRRELGDWLVALTEKIAPRSA
                     VHARLVERMIFAARLEGVHEAWRDAQAGAAWAGARTGVGAEA"
     gene            8296..9429
                     /locus_tag="PLS90_00950"
     CDS             8296..9429
                     /locus_tag="PLS90_00950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK00998.1"
                     /translation="MSPEADTRSAGGARCTVGLLTWNGGDAAEPCVRSVLAQTERPLP
                     IIWIDNASTDGTVERLRRAFPALPEPIVNARNIGFTGGHNQAAARCATPYYLALNQDV
                     VLAPEYVERVCAWLDEAPDLALASGLILRGAGPPGEQAPTAPGPDDRIASAGIVWPRL
                     RFPFELALGWPVEEVLRTRRWVPGVNGAAMILRLAACRAAAIEPQPDPAGGEPLPGIL
                     PPDFFAYHEEFDLALKLARMGWRAGVEGRAYAWHGRFSSGGLRRRSIRARALLNHWLL
                     TLRHDDWPTILKELPYLLKGELQFWLPRYLMYPGAFLEAVGGLARGAAAARRFHRRFT
                     AHAAAADRPDRLAEYRRESLRQLRQWKPRRTVAEKLRRTGGSF"
     gene            complement(9506..10765)
                     /gene="clpX"
                     /locus_tag="PLS90_00955"
     CDS             complement(9506..10765)
                     /gene="clpX"
                     /locus_tag="PLS90_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPK00999.1"
                     /translation="MKLRCSFCGKDQHSVRTLFKGLTQKESMMTVYICDECVGQCHER
                     LKRDQILQAEEAAFETLKTLPSPSEIKSAIDQYVISQEKTKKTVSVAVYNHYKRLMHN
                     LASQDIELEKSNILMVGPTGSGKTLIAQTLARILEVPFAIADATTLTQAGYVGEDVEN
                     ILLRLLQNAGGDKEKAERGIVYIDEIDKIARTTQNVSITRDVSGEGVQQALLKILEGT
                     VANVPPTGGRKHPHQEYIQIDTRNILFICGGAFSGLDKIIEQRVQQKTMGFGAEVRSR
                     KDYRIGEILEQVNYYDLLKFGMIPEFLGRFPIVCTLDQLDEPELIQILTTPRNSIIKQ
                     YQKLFEMDSVELHFSDEALHEIAHKAMELRTGARGLRAILEEIMLDIMFSLPEKAEEY
                     DECHIDGGVIRGEKEPLFIQSEKRESA"
     gene            11411..13297
                     /locus_tag="PLS90_00960"
     CDS             11411..13297
                     /locus_tag="PLS90_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01000.1"
                     /translation="MSATSPTVYRSLWFWMRGHRLRYGAAVLALLLSVYFSFQVPLVI
                     ADVIDAIFREGAADREPSRFVKLLLRVVGSDGLRRHLWLPGLLMVGLMLVAGGFSYLR
                     GLWIAQASESIIRAMRERIYDHLQHLPVAFHAAADTGDLVQRSTSDVETVRNFLGGQV
                     TELGRGIIMLIVLIPIMLTINWVMTLYALSLTPLIILASLFFFSRIKAAFKEQDEAEA
                     AMTTVLQENLTGIRVVRAFARQEHEIAKFQGKNAAYRDLQYRVVCLMAWYWPSSDLMC
                     LAQTGFVLVVGARFVQHDALSVGQLFFYMACLGMLLWGPLRNMGRVLVDMGKAQVAIG
                     RIEEILREPRESGPARPMALPTEMRGEIEFDRVSFAYQDGQPVLHEVSFRVEPGQTFA
                     ILGPTGSGKSTLVSLLLRFHDPAAGAIRIDGVDIGQAERKAVRARIGVVLQEPFLYSK
                     TLRDNIRFGHSEAPRERIEAAAIAACVHDNILDFEKGYETEIGERGVTLSGGQRQRVA
                     LARAIVKDPPILILDDALSAVDTHTERLILRALADRHGRKTTILIAHRLSTLMHADRI
                     LVLDHGRVIQTGSHAELVNQEGLYRRLWSIQTSLQQDLAGDPPAPAAEARAALAQAEA
                     PTHV"
     gene            13290..15206
                     /locus_tag="PLS90_00965"
     CDS             13290..15206
                     /locus_tag="PLS90_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01001.1"
                     /translation="MSETVFREEEFNKRLDPRLWWRILQFARPYRPLVGGLMTIALLV
                     GLTESLYNLLVKRVIDGLVEFGPDAPLFSYALQYAAITITLVVCVFIFIRFAGKLSAF
                     ISHDIRRAGFARLQELSFSFYDHRPVGWLMARMTSDCERLSSVLAWGLLDLTWGTAFM
                     SAIAVILFYLNWKLALIVFTVVPVLAWISLHFQRVILRSSRLVRKINSRLTGSFNEGI
                     SAVRTTKTLVREEGSLREFASLAGEMRRASIRNAVQSALYFPLVITIGSAGAGLAIWF
                     GGRDVLAGVISLGTLVAFVSYSRDFFMPVQELARLFTNLQMAQASAERIIGLVDTEPE
                     IRDSAEVRTSIARAGAQPPVAGRAIDGLPDRIETIEFRGVSFAYNVRKPMGADDASAG
                     EREVIKVHPVLDDFNFQVQAGQTIALVGPTGGGKSTIISLLCRFYEPTAGEILINGID
                     YRRRSLHWLQSNLGIVLQSPHLFSGTIRENIAYGRLEASDEEIVRAAQLVNAHEFIMA
                     MPGGYGAQVGEGGVNLSTGQKQLISFARAILADPQVFVMDEATSSIDTETERLIQAGI
                     ERVLSNRTSFVIAHRLSTIRRADCILVIEKGRIAEQGTHEQLLAARGHYHDLYTHQFT
                     REAEEQVLADPLLA"
     gene            15444..16679
                     /locus_tag="PLS90_00970"
     CDS             15444..16679
                     /locus_tag="PLS90_00970"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPK01002.1"
                     /translation="MGLIVQKFGGSSVADVDKMKLVAGRVIAECDRGNQVVVVVSAMG
                     KTTDNLLSLIAQVTDRPNPREVDMVLATGEQISIAILALVLQTMGRKAISMTGPQVGI
                     ITDPFHRKARIRDINTARLREKLDEGHIVIVAGFQGLTQSGQITTLGRGGSDTTAVAL
                     AAALHADRCDIYTDVDGVYTTDPRIVPDARKLATITFDEMLELASLGAKVLHNRSVEL
                     ARNHNVPLQVLSTFSQNPGTLVVKEYTNMEDIVVSGVAYNRDEYKITILGVPDQPGMA
                     AKLFSKIGEANVNIDIIVQNISHRGLSDISFTVGKEDFQNARDVAEQFVKEYGAEGIR
                     TDDAVGKVSIVGVGMRSHGGIAATMFGALAKENINIQMISTSEIKISVVIARADVDRA
                     VQAIHREFELQSEPKLTAR"
     gene            complement(16684..18483)
                     /gene="ptsP"
                     /locus_tag="PLS90_00975"
     CDS             complement(16684..18483)
                     /gene="ptsP"
                     /locus_tag="PLS90_00975"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPK01003.1"
                     /translation="MTPTAPKPASRTYQGIAVSPGIYIGKIHVYDPVQTVVPHHLVSA
                     RAIENEKKHLREALAKAKEELQRLRERVRDTLDEAHASIFDPQLMILEDPAVVDSAVE
                     RIEKNQENASRAFMTIIRQFVDKFSSVGDPYLASRNSDILDVGNRVCQHLAQVQPESS
                     SALQFDSEVIVLARDLSPSDTAQMDQLHVKGFATELGGPTSHTAILAKALEIPAVVGL
                     GPFLAGLKTGTQTIIDGYEGTITIDPSPREIARARQRRKKHLVHERYLQKLKHLPAET
                     IDGYRVDLAANVELPIEIPHVLAHGAEGIGLFRTEFQYLENDRLPGEEELFEVYRKVV
                     EVMDPHPVIFRTIDLGGDKFLGALQTGRELNPFLGLRAIRLCLAHPEVFRTQLRAILR
                     ASAHGSARIMFPMISNVAEVRQAREMLDEVAADLRRERIPFDEEIQIGVMIEIPSAAI
                     TADQLAREVDFFSIGTNDLIQYTLAVDRGNEQVAHLYEPFHPAILRLIRNTIDAAQRE
                     GIWVGLCGEMSANPLCALLLLGMGIDELSMGALAIPEIKRLIRQVELTDARRLVEEVY
                     SLRTAEEIREHVLQAYRGLQRKRARAGGMLRAI"
     gene            complement(18588..18875)
                     /locus_tag="PLS90_00980"
     CDS             complement(18588..18875)
                     /locus_tag="PLS90_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPK01004.1"
                     /translation="MEQTQTGHRATVTIPNSLGLHLRPASQLVKIASKYEKCDVFLTK
                     DGQSINAKSIMGVIMLAAEQGSELILEANGEGSEEAIRELVALIESGFGET"
     gene            complement(19211..20572)
                     /locus_tag="PLS90_00985"
     CDS             complement(19211..20572)
                     /locus_tag="PLS90_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK01005.1"
                     /translation="MVSFITDRLRQLSPSGRQTLPAHFVLALSSAPQLMMVTYAPFLM
                     TKTFQTDYWVPVLAATFHAGNILAVVFSNRVQRSDKLGWVLWPNCIAFCLSFLLLPVR
                     PSMDAYFAVIVMLMQLSRAPVVSALSALIKTNYRPAIRSWVMSMLVSAQMLAIGAYSF
                     LAGQALEHSEQWLGPLYAVAGGIGLVGLWNFSRLRLRKEAAAAARPEPGTTRVSLWEQ
                     FCILGRDRGFRNFEVSFMLFGAGNLALATIFPLYLKAEFNASYEQAATALQTLPTLIT
                     FLMLPVWGMWLDRNNPLVMRFRINVLLMLVPIIYYFSRSMPAIYGGVLVTGLMQGGGM
                     LIWILGVNYYARSRDIALYMGLHQSLTGMRGVTMPFIAYTLGSWLGYRQTMLVWAAMM
                     GAGALILQREIGRERRLGILRNFEEREAQHQQADAREADAGKSRPVSPDEEASGDDAP
                     FGR"
     gene            20993..21658
                     /locus_tag="PLS90_00990"
     CDS             20993..21658
                     /locus_tag="PLS90_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005986880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2959 domain-containing protein"
                     /protein_id="HPK01006.1"
                     /translation="MFQPPHRFIGAILLVVALGGAAACQRAYYSVMERFGVPKREILV
                     NRVVEARDAQEEARERFQSALERFQTVLGFDGGELEDKYNALNAELKRSESRASAVHD
                     RIDAVRDVAGALFAEWEDELDQYENADLRRRSERQLKETRDRYQQLITAMERAESRIE
                     PVLKPLRDQVLFLKHNLNARAIASLQEDLGEIQTDVDALIRELERAIAEADAFIQQME
                     TAE"
     gene            21845..22759
                     /locus_tag="PLS90_00995"
     CDS             21845..22759
                     /locus_tag="PLS90_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01007.1"
                     /translation="MRWATYHGATPWLLLVVLALLCNVVGMIHLIGLRLVSLRKLNLT
                     GEPQLFGLRRAVLNDLLLTELTPHDFAVGYWGQQLNGRAVRRSRVILGVSQFVTGSVF
                     LGVLLLAPPDGGREVWLECISVAAAIVGWQAGVARYEPWSSLPRLTRRVQQQRRLFGT
                     TQRERITSLLASEAAIYIALLAVGLFVLPHVLLVLAGILVGAKLHVLLTHPLGPPAWA
                     VAGELLGMREGWRRAKSAPRLLQRIEWDFHYIFYFHRWGEDEVAAREAPGSAPAPAAR
                     AESVPDRYRAPKSPIRADAQTPPEKSEA"
     gene            complement(22782..24554)
                     /locus_tag="PLS90_01000"
     CDS             complement(22782..24554)
                     /locus_tag="PLS90_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012165466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HPK01008.1"
                     /translation="MTDLSAVQVWTYVLVTVTFMLYLYIGWWARVRESSGFYVAGRGV
                     PAIANGAATAADWMSAASFISMAGLISVMGYDGSIYLMGWTGGYVLLALLLAPYLRKF
                     GKYTVPDFVGDRYYSDTARVVAVIAALFISMTYVAGQMRGVGIVFSRFLEVPINVGVV
                     IGMVIVAFFAVLGGMKGITWTQVAQYGVLILAFLIPSSALALKLTGQPIPQLAFVFSD
                     LTEKLNQIQVDLGFKEYTEPFRDKSLLDVLCITVALMVGTAGLPHVIVRFYTVPNVRA
                     ARWSAGWALLFIALLYTTAPALATFARYNLLASLNGKTIEEIEQLEWVASWEKTQLLK
                     YVDKNGDGRIQIAPGDAFLRTDGDLNLAQPNPATANEVYFSADIIVLSTPEVARLPAW
                     VIALVAAGGLAAALSTASGLLLVISSSIAHDIYYRLIRPTASEGQRVMVGRICVVLAV
                     IVAGYFGINPMGFVAQVVAFAFGLAAASFFPIIILGIFDSRTNRTGAIWGMIVGLSFV
                     VIYITGTQAHKIFGTDQPWFGNWFFGISAEGIGAVGMVLNVVVTLVVSRLTAAPPRAI
                     REMVESIRHPDHPAPALILDEESE"
     gene            complement(24600..24932)
                     /locus_tag="PLS90_01005"
     CDS             complement(24600..24932)
                     /locus_tag="PLS90_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HPK01009.1"
                     /translation="MANPRSETSTPSPNDKHRHLLDPYWRANQRLIWTLLAIWALVSL
                     GAGILFVRQLNAIQLGGLPLGFFFAQQGSIYVFVLLIFFYAWRMDRLDHKFRLDVDAD
                     PTPPPQAH"
     gene            complement(25151..27514)
                     /locus_tag="PLS90_01010"
     CDS             complement(25151..27514)
                     /locus_tag="PLS90_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPK01010.1"
                     /translation="MSEFRRASARRRCPPRLPFRALLFGWTLAIMTAAAASAQGEGAR
                     VDAVRIDGLAGYRPEQILPRLQTQPGQVYDPVEMREDIEELGKIFRTVNVQTETTPGG
                     GLDVVFTVSEFPRFRELQVVGNQKLKTERIERLVGMERGSLLDAATLDRIRRALRNEY
                     KALGMPQARVTLNLIDQPAEQTGDGAPIPQADLQIVLDEGRQVLVDDVLIEGNDAFSD
                     FRLKGMLQTKGSVLIVKNYYDEETFAEDLDRLRDFYAAHGYFDAVVQRGMFVERTGGK
                     IPVVSPVIQVNEGVRYTFGDARVRGARLFSHAEVAAPFEPLKGELFDARRFAKALDEL
                     SALYYNHGLLTTEITPEYQYDTENQVLNMVIDVDEKSRIYVGRVRVERPPLAEDEEPS
                     WFRGWYDRMAPPVKNSVILREVLLEPGEVYNKRLERETVRRLSRWGIFENVNARNEAT
                     AEPGVHDLVLSLRESTTGSISGGVGFGDAAGAFVYAAFNERNVGGRADVFGFNGRLGT
                     RESELSITYLDRHFKETRDSMAYRLFYESMGRPGYRANVAGFHTEWAHPLEASDWTMY
                     VRGRLEGVDLNEREHDDDLEDELDRSYAVLTGRLKFIEDTREPVGERPREGYIQAAGA
                     EIGYAGGPLVKLEAERDQYRPVRERMTWRLAMMAGLMPFSADLVPIHERYFLGGNTDM
                     RGFAYRGAGYFDGDDDDIPVGGGAKILVKNELHYPIVDPISGLLFVDVGTLGDSPVSW
                     QVPRVSLGTGLRFDMRNVQVGLDLAAPIVSHGDDETRFFHFSLQSAF"
     gene            27898..29370
                     /locus_tag="PLS90_01015"
     CDS             27898..29370
                     /locus_tag="PLS90_01015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01011.1"
                     /translation="MARLLVAGIGPLPPEQPGRLFAPGLRIWGIARELARAGHAVRLV
                     RGRFGDGAAGEAALFDLEPAAGAPRGPAELPPMRTRRVAEGGWPQLLAHEATQFNATA
                     AVGSTDVMNHALAATGLPLPIWMDYFGDPMAERQMLALAHGSDQGLADQWALVAPALA
                     RADRLSGCSREQCAALRGQLAAVGRLGRLTATEPLVHEIPPWIEPVGDYVGGARPAER
                     LLRGVVIPEDSFLVVQTGGFNTWLDVKSLFSTLERVMADEPRLHFAATGGPIAGHHES
                     GFEWFQAAVGASPHADRFHLLGWRPLADVPRLLAEADLAINLDLPCIEGRHGTRNRLL
                     DWLLAELPVISTPGCELAEELGQAGYLLLTPHGAADRAAAAIRKVMAGAEEFRQAATR
                     GAHYLREVHAPERCLAPLLAWAAEPKPASDLHLWRRGTAAPSALHAAAVDAAALACRL
                     AAEQAQRLRLEQELAELKGSRLIQWALRLRRGGPSRPRAW"
     gene            29492..31093
                     /gene="pilM"
                     /locus_tag="PLS90_01020"
     CDS             29492..31093
                     /gene="pilM"
                     /locus_tag="PLS90_01020"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPK01012.1"
                     /translation="MPRPICGIYLTNEQVNALCLRPTRKGLALTGADAAPLPEGDDDA
                     LGSALKPLVKSLGLAGAEAHICLPKQQAILRDVTLPSTQPDELSQMVRYEAERHIPFH
                     ADRHCVGYHVMKSRGIEGSEVLIAAVDGPVVERALSGVAEAGLRVRGVTLSSVALMNS
                     LLHVRREWMEGKTIALLCLGLDTVDLALVHQNNLIYARSVVMDLRSVLKSWLGLDPAT
                     PDREVEIEKLATACRMIDCLDLEAQYSESKSVAREDHVALVRGWLNRLVQELQRTYDF
                     ARREMKCPQITAVVLTGEGALLRNIERYLYVNLNVEIQTLNPVGELTRSLKKKMPFDG
                     LEFVIPCGAALTDEMTGGYRIDLTPREFYRRMARRQTLRQLSLTGVLAAITLVLGVLS
                     LFHLREIQARELKDYQDANRQLRPAVNELEEKRTKLGIIQGFLNPRNSALNVLLTINR
                     TPGIASRVRLTRVVFEKGETVTIEGTAKTVPDITTFAGDLRQSGQFASVTIPRQEPRE
                     LRNLGETVWNFSIEAPLLSDAGGDR"
     gene            31090..31674
                     /locus_tag="PLS90_01025"
     CDS             31090..31674
                     /locus_tag="PLS90_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01013.1"
                     /translation="MNRRERILLIVTVLIVAPLLIYHFFLADWLEGMAGQAGGDLSVE
                     RARFASNIATLADRRQIETAYSRIDFSQSQPLPGQNATEHFLNELYGLLTEEFQIMNP
                     RIEKARPQAIPDVPEYYFVELEVTVEGSPEEMLRLLVNMENRGVLIKSLTIDTRSGGG
                     FGRDDELVNLTVEVARLVKHTEESMKMMRGFGSF"
     gene            31900..32229
                     /gene="trxA"
                     /locus_tag="PLS90_01030"
     CDS             31900..32229
                     /gene="trxA"
                     /locus_tag="PLS90_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HPK01014.1"
                     /translation="MASDKVLHLTDESFDTTVNNPDVPVLVDFWADWCGPCRRIAPMI
                     DELADEYAGRLTVAKVNIDEHQGAATQCGVQSIPTLILFKGGQIAERIVGAVPKAILT
                     EAIDKIL"
     gene            32314..32922
                     /gene="pdxH"
                     /locus_tag="PLS90_01035"
     CDS             32314..32922
                     /gene="pdxH"
                     /locus_tag="PLS90_01035"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HPK01015.1"
                     /translation="MSLPPLLEHDADPDPIAQFSRWYAEVEAAGQAEPTVMTLATATP
                     EGVPSARMVLLKHAGPDGFDFYTNYDSRKAAELAANPRVALVFWWERLRRQVRVEGTV
                     VRLPSAASAAYFATRPRGSRIGAWASPQSRVLAGRAELERRVAEIEQRFAGQDVPLPP
                     FWGGYRVHPWIIEFWQERGDRLHDRLRYRREGDGWVMERLAP"
     gene            complement(32960..33979)
                     /gene="galT"
                     /locus_tag="PLS90_01040"
     CDS             complement(32960..33979)
                     /gene="galT"
                     /locus_tag="PLS90_01040"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPK01016.1"
                     /translation="MPEFRKDPVTGRWVIIATERAKRPAAFPVKRQPTHSNEECPFCP
                     RREHMTPPEVLAYRQPGTDHNEAGWWLRVVPNKFPALVSGEPVKRSGDGMYDRINGMG
                     MHEVVIETPRHDEHVGDMEVKMVEEIIWAYRDRTVEMRKDPRLEYVMIFKNHLREAGA
                     SIDHPHSQIIATPIVPKRVQEEIDGAQRYFDYKERCVFCDMIDQEKKDQMRLVAENEL
                     FIAFLPYASRFPFECSIMPKEHTSFFHDIQKNEVTEFAKVLKETMSRLKNVLDDPAFN
                     WILHTTPLRETENGYYHWHMEIIPKLSQPAGFEWGTGFYINPFPPEDAARILKLDGAA
                     EQAAG"
     gene            complement(34458..36497)
                     /gene="tkt"
                     /locus_tag="PLS90_01045"
     CDS             complement(34458..36497)
                     /gene="tkt"
                     /locus_tag="PLS90_01045"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPK01017.1"
                     /translation="MTTPSASTRLAPLTSQDKQILQKVADTVRVLSAEAVEKAASGHP
                     GLPMGCAELGAYLYAYALRHNPRNPGWMGRDRFVLSAGHGSMFLYSLLHLSGYALSLD
                     ELKNFRQLGSRTPGHPEHEAHAPGVETTTGPLGQGVAAATGMAIAQKWLRARFGAELF
                     SAKIVALAGDGCMMEGITSEAGSLAGHLGLENLIIIYDSNDICLDGPLAEAMSEDTAR
                     RYEAYGFNVLRINGHDFDEIKKAFATAWDENTRPTLIIAKTIIGKGAPNKQGTSASHG
                     AKLGAEELAATKKALGWPDEAFHIPTEVREFFKSRQAQFAAYEKAWNEKLAALMQDPE
                     KAKLWETCANGELPADYADAIWNLDVAPNKATRAQGQQLIAKVAELAPFFVTGSADLS
                     CSDLTNIKSSQFVTRADWTQRNIKFGVREFAMAAANYGMWLHGMVQPLCGTFFTFSDY
                     MRNAIRVAALMKARVFFQFTHDSIFLGEDGPTHQPIEQLAALRAMPNLTVFRPCDENE
                     LKAMWIEAWKCDGPVAFILSRQGIESQGEATRSRAREGVARGGYVLKGEPGGRCDVLI
                     AATGSEVGLAMRAAALLEQQGRSVRVVSMPCWERFEAQDAAYREAVLGGEVGLRVSLE
                     AAATLGWHKYIGADGLAIGIDQFGMSAPEKALAEHFGFTPEKVVERITAALPVGA"
     gene            complement(36664..36990)
                     /locus_tag="PLS90_01050"
     CDS             complement(36664..36990)
                     /locus_tag="PLS90_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01018.1"
                     /translation="MNATIFMLLPFLVVGMFVFVIGAIILVLVNRRKAMQRAWTGQIT
                     RKYTSKDRRSAKHGRDLPHYYIYYRSDGGEEGRLAVGEAIFHSLQVGDRVVKEPGKYP
                     AKAPAA"
     assembly_gap    37065..37164
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            37478..37960
                     /locus_tag="PLS90_01055"
     CDS             37478..37960
                     /locus_tag="PLS90_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK01019.1"
                     /translation="MSIISRTAQYALRSMTLLAIQHDRVTVKDLAQEVGAPQPYLARI
                     IMLLADKGLVDARRGPGGGLRLARPAEEIRLIDIVSLFDGSKLFEECALGLPGCREGE
                     HPCPIHDQWGCIREQIRTWWHDTTLAQFKPEQTLECMRERVKHLPVETPYSRGNGRWR
                     "
     gene            38202..40070
                     /gene="dnaG"
                     /locus_tag="PLS90_01060"
     CDS             38202..40070
                     /gene="dnaG"
                     /locus_tag="PLS90_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPK01020.1"
                     /translation="MADRLEITPAQFAQQVKDRLALDQLIAEYVPSLRRSGNSLKGLC
                     PFHKEKTPSFNVHVELGFYHCFGCGAHGDAIKFVQEIEKIDFMLALEQLARRAGLQMP
                     RFGRGEGPARPEREEQLARLKAACAWAEAFFIEQLEQHPRGAVAREYLRGRGLTDEEI
                     ARYRLGYAPDGLDVLLREGERRGWSGETLAEAGLASRREQGGFLDRFRDRVIFPIADR
                     YGAVVAFAGRLLDSEREAPKYINSAETPLFSKSRLLYGMALARDAIRESRQVILLEGY
                     MDWIALHRRGLANALAGMGTALTEDQARLIRRIADRATLIYDGDEPGQKAAFRGTELL
                     LRQGLEVRIVTLPAEHDPDTFLEAEGTQALRERIAEAPAAVSYFTARIGERANLSRPE
                     GKADAVQQIAPLLLALGDPAIREGYLEQAAARFGLRYETLEAALRRRAPRRVGPAAEA
                     GQVDAGAAPGEPGGDWPVTEQILLFSLLHFEARWELIQQIDAEWFTHEELRGLYQRIY
                     EAHRDVREGADPPTDWFALCHTPAEEALLSRILLLPQTHFRGDVADFSHTLADAIQLQ
                     VLKLKRNWTQRKRRELARDLQMILDEHPLGRGQLAAIDELTRRQLQETAEFFRRNE"
     gene            40457..42208
                     /gene="rpoD"
                     /locus_tag="PLS90_01065"
     CDS             40457..42208
                     /gene="rpoD"
                     /locus_tag="PLS90_01065"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPK01021.1"
                     /translation="MAVQEKNPFEAIIELGKEKGHLTFDEINERLPEDITTPELEVLF
                     EELDQLEIPVLEEEAEEESEAPAREETFERDEYQDEQAAADLEAAKIDDPVRLYLMEM
                     GKVPLLTRDEEVSLAKQIEHGRHMITRAIARASVTADEIRSIHERLENNLYNLNDLLR
                     ANVDEANADERAKVVRRTLDQLDGVLSIYNQIDQDLEAIEDAALSGEDPAALEKAVEE
                     RREAIYQKLLEIDLNYAVIEQIAAKIRTVFTRIDEANQEIQSVIVKTMISEEELRRIV
                     KRTKRNSPEAKALPRKYGFTLDDAIKMDKLVRNAQKTIKRMEKEVGNSYEELQEIIRQ
                     IDVGEKEAHEAKMKVVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMRAVDKFEYQ
                     RGYKFSTYATWWIRQAVTRAIADQARTIRIPVHMIETINKLSRISRYLVQELGREPTP
                     EEIAAKMDMPVEKIRRVFKIAQQPISLETPIGEDGDSHFGDFIEDQDATSPVTATAYR
                     LMQEKIEEVLGTLTEREEKVLRLRFGIGNNDFPRTLEEVGTIFNVTRERVRQIETKAL
                     NKLRHPSRRKRLIEFME"
     gene            complement(42291..42695)
                     /locus_tag="PLS90_01070"
     CDS             complement(42291..42695)
                     /locus_tag="PLS90_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01022.1"
                     /translation="MLRWLFFWFRPARLLWPVACLALTAAAWLFVNYDTLQRYLSERE
                     RLEHYRSLVAEEERLRDEMIGERAALQAGGFPAEKAIRERFIMVKPGEQILFIEPPAG
                     KDEADAAGGDSKPSAPGKERVDPAEPVLPETD"
     gene            43105..44820
                     /locus_tag="PLS90_01075"
     CDS             43105..44820
                     /locus_tag="PLS90_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01023.1"
                     /translation="MNSAPPHSSLREAIPNRRQLALDLTLLAAMLAVGLYLRLANLTL
                     VQHFQGDQGRDYIVVWDWIRAGRWPLLGPYRGVAGDFAIGPGYFYLIAPLMVLTGFHP
                     VSGPATIACMNALMVVLAWRWVLLSTGSRLAALFVPFTILFSAVWTVTDRYNWNPNTL
                     PLATMLMAALIFGLRRRPVPCLTGYVMLCALLPHLHTTTLLMIAVSLPIVLWVLLRER
                     PDWARDRGHRRWAWIALAAVWLALLYTPPLIYEIGPRTNLKGYIARSIPPSPPPEGPP
                     LARAHDALDRLGRWTMHHNWPNRWPVHYPRNVLMLASILAGAAAMLAWSVGHRRARRA
                     DGEWAVLYLLLQLGGMLYVAFQKGDNFEQYYANQILAIPVLLLGWLLGMLLSGMARQL
                     GPAGRRRAAVVWASRLLAFAILVACGRWTVTQLPDAIGVHRGKVWYEFQFVRTQEIAR
                     AIINAARGRPYSFQYFGPGGVRDHFEYLLRYMGNGPRNDPGDGRRIRARDLGSALFIL
                     TQNEANAWRPWFESAATPATPSPLRIHDANIYEIPRSAMPLELRWVEVVERDGRIIIR
                     WRERD"
     gene            45031..>46032
                     /locus_tag="PLS90_01080"
     CDS             45031..>46032
                     /locus_tag="PLS90_01080"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK01024.1"
                     /translation="MTSAHTTTGRPLRERVIALIDRGVSLPPLSPAAAQLIRILPQPI
                     DRIDMRKLVELVQEDPTLSAHVLRLANSPFWGIRQSVSTVGLAVSLIGLQEVVGTLNY
                     HLLRQLTPNVKRSAHFSPDAFWLHSWRGATAARLLGRPDLFVTCLPGQLYMAGLLHDI
                     GKLILVLHFGEEWEACADEAATSGRPLHEVEQERMGVDHAEVGARQLEVWGLPDLIRV
                     AVDWHHRPGELPEGPAREMASLIQFANAIAHACTAHEVGTVPVCPISETWLGMHKDHP
                     LSDPVKREGIVHEVMAALLARERHLDDGEATNAAEEEPARPAPIAAPRPVELAPARQW
                     "
BASE COUNT         7540 a        14995 c        15658 g         7739 t
ORIGIN      
        1 cggccgcctt ccgcatctgc cgcggcacct cctgttttct ctcgcgcggc agcgaaacga
       61 tccacattca ctcctcgcat gcgctcgccc acgcgatcgc ctgcgccggt tactgcgacc
      121 gggccccggc cttcctcgac cccgatggcg aggtccatac cgcctcgcgg agccgcccgc
      181 gccatgccct gcccgacctc cgggtgatgg gccgcgagcc gctgctgctg gcgcggctgc
      241 tgcggggcga tcacgcgcgg ctggaccagg cgcgggccgc gggcgtctac gctgcgcttg
      301 cccgcgcgct ggagcggccg ccggccgagg tgttgcgagc cgttgaggaa tcgggaatcc
      361 gcggccgcgg gggcgccggc ttcccgaccg ggaccaagtg gcgcgcctgc gccgaggccc
      421 ccgggccgct gcgctgggtc gtggccaacg gcgacgaggg ggaccccggc tcgttcatcg
      481 accgcgtcct gctggagcac gacccgcacg ccgtcctgga ggggatgctg ctgtgcggct
      541 acgccgtcgg ggcgcggcgc ggcatcgttt atatccgctc ggagtatccc tccgcccgcg
      601 aggtgatgcg gcgggcggtc gccgacgcgc gcgccgccgg cctgatcggg cgcgatgtgc
      661 tggggagcgg tttcaccttc gagatcgacg tgatcagcgg ccacggcagc tatgtctgcg
      721 gcgaggagac ggcgatgctc aacgccatcg agggtcgccg cggggaagtc cggctgcgcc
      781 cgccgtttcc ggcgcagacc ggcctgttcg gctgcccgac ggtgatcaac aatgttgaaa
      841 cgctggccaa catccccgtc atcgtcgagc gcggagcgga cgcctatcgc cggttcggca
      901 cgccgcagag cgcgggcacc aaaacgatct gcctgaacaa tgcctttgcc cggccgggga
      961 tcgtcgaagt cgaattcggc gtctcgctgg ccgaggtgat cgacgaggcg gggggcggcc
     1021 gcaacggcga tgcgcttgag atgatcctga tgggcggtcc gatgggcagt ctgctcccgg
     1081 ccgacgagtg ggacgtgccg atctgctacg gcgcgatgcg cgagcgcggc ctcaagctgg
     1141 gccacggcgg cctggtcggc attccgcggg ggaccgacgc gcgccggctg ctggaacacc
     1201 tgctggaatt catggtggcc gagtcgtgcg gcaagtgcgt tccctgccgg ctcggctcgc
     1261 ggcgcggccg gacgcttgtg gcccagctcg gggccaacgg cgaatcccgc gccgagttca
     1321 cgcggctgct gcgcgtcatc gaggcgggca gtctgtgcgc cttcggccag catatggtcg
     1381 atcccatgcg ccgcctggtg gaactgaagg gcgacgagat tttcgcgggg gcgtgcgcgc
     1441 gatgagcgag gcgacagcaa gcatcgacgg ccggaccgtg gcggtgcgcg acggccagac
     1501 gatcctccag gcggcgcgcc aggcggggat ccagatcccc acgctctgct acctggaggg
     1561 gctgcggccc gaggggggct gccgggtctg cctggtgcaa ctggacggct cgccgcggcc
     1621 ggtggccgcg tgccacacgc ccctgcgccc cggcatgcgc gtcgcgaccc gcaatgagga
     1681 gctgttcgcg ctgcgccgcg acatcctcag cctctacctg caggacggca agctgaacgc
     1741 ctaccaccgc gaccaccgcg attcggaatt cacgcgtctg ttggaggacc acggcctggc
     1801 ggcgccgcgg ctcgaccccg cccgcgccgg cgcccggctc gaccagagcc acccctacat
     1861 gcggttccag cccgcgcttt gcatcaactg ccgccgctgc gtcgccgcct gcgacgaggt
     1921 ccaggggcag ttcgtctatt cgatggccga gcgcgggccg ttcacgcacc tgatctatgg
     1981 gcgcgacggg catttcctgc acagcccgtg cgtcgcgtgc ggggcgtgcg tcgatcgctg
     2041 cccgaccggg gcgatcagcg accgcgatcg cctgcggggg gaagcgcccg agcgggtcgt
     2101 cgagagcgtc tgcggctact gcggcgtcgg ctgccggatc gccgtggccg cgcgcgacag
     2161 ccgcgtgctg tcgatcaccg gcgtcgccgc ggcggccgtc aatcgtggtc acctctgcgt
     2221 caagggccgc tacgcgcacg cctatcatca ctcgccggag cgcctgaccc agccgctgct
     2281 gcggaccgac gacgggttca aaccgattcg ttgggaggag gcgctgcggg tgctggtcga
     2341 ccggctgcgg ggggtgcgcg aacgacacgg cgcccaggcg ctgggggtct ttacttcgtc
     2401 gcgctcgacc aacgaggcca cctacctgct gcagaagctc ttccgcaccg tgatcggcac
     2461 gaacaacgtg gactgctgcg cgcgcgtctg ccactcctcc acggcgctgg cgctccagca
     2521 ggtcacgggc accggcgcgg cgaccgccgc gtatgaggac atcgaccgct gcggctgcat
     2581 cctgctggcg ggggccaatc cgaccgaggc gcatccggtc gtgggcgccc ggctcaagca
     2641 ggccgtcctg cgcggcgcgc ggctgatcgt ggccgacccc cgccgcatcg agctggccga
     2701 ctatgccgac gtccacctgc aactgaagcc gggcgccaac gtcgccctgt tcaacgcgct
     2761 cgcctgggtg ctgttcgatg aaggcctggt cgacgcagcg tatgccgctg agcggctgga
     2821 gggtttcgcc gaactgcagc agttcctcgc gcgccatgcg ctgaccgact tcacatcgac
     2881 ggcgggcgtc gagccgcaac aggtccgtgc cgccgcccgc atcctcggcg ccggcggccc
     2941 ggtgctgttc gttcatgggc tgggcctctc cgaactgacg cagggcaccg cgtcggtcaa
     3001 ggcgctctgc aatctgggga tgctgaccgg cagcatcggc cggcccggcg gcgggatgct
     3061 gccgctgcgc ggccagaaca acgtgcaggg cagcgccgac atgggcagca tgcccaacca
     3121 actgaccggc taccagccgg tgggcgatcc tgaagtgcgc cgccacgtcg agtcgatctg
     3181 gggcgagctg ccccccgcgg agcccggcct gacgatcccg gagatgctga ccgccgccgt
     3241 gcacggccgg atccgcgccc tctggattca gggggaggat atcgcccaga gcgatccgtt
     3301 ccagcagcat gtccgcaacg cgctgtcgaa gctggagttt ctggtggtgc aggaactgtt
     3361 catgaccgag acggcgcgct acgcgcatct ggtgctgccc gccgccgggg cgctcgaaca
     3421 ggacgggaca ttcaccaacg gcgagcggcg gatccagcgc gtgcgcgcgg cagtgccgcc
     3481 gccgggtgag gcgcggcccg actggctggt tgcgcgcgac gtcgcgcgcg ccttcggccg
     3541 tgactgggat tacccgcatc cgggcgccgt gatggacgag atcgcgcgcg tcgccccgcg
     3601 gctgttcggc ggcgtccgct acgaccggct cggcgccgac ggcctgcaat ggccctgccc
     3661 cagcgtcgac catcccggca cgccgcaggt ccaccaggcc gccttcctgc gcgggcgcgg
     3721 ccagctctcg gtcaccgagc cgctggccaa ccccgaacac gacgtcgccg gcttccccta
     3781 cacgctgatc accggccgcg tgctcaacca ttacaacgtg gggacgatga cgcggcgcac
     3841 gccgcagagc gagctgaccg acgccgaccg gctggagatt catcccgacg acgccgcgcg
     3901 cgaggccctg aaggacaacg accgcgtccg gatctgcagc acgtggggct gcacgcacgt
     3961 gacggtccgc ctctcgccgc gggtcagccc cggcacgttc ttcctgccgt tccactttcc
     4021 cgagacggcc accaatcagc tcatcggccc gcacgccgac cccgattcca agtgcccgca
     4081 atacaaggtg accgccgtcc ggctgagccc gccggtggag gaaccgcgcg gcgaaatcgc
     4141 ggccgaagcc gttgcggccc ccgatcggcc atccaatggg gatgcacacc ccggccccgc
     4201 cgcgcgcgaa gcggaacgga acctggcggg cgccagaacc tgagcctgaa gaatgatttt
     4261 atcaagatcc gacggatccg acggatctga cggatttcct ggcggcgttc ccgcgcaaac
     4321 aaaaacccgc cgcgaccggg ccaacgggcg gggtcgcggc gggcaagtgc gttcaatccc
     4381 tcgggggagc gatcaggcgt agacgcgctg cggctcctcc tcgtcttcga gggcttcttc
     4441 ttcatcctcg tcgtcgagcg cggcgtcggt ggcgagggtt tcctccagca cggcggcctc
     4501 gccggcgcgc tggcgttcgc ggcccagcgc cttcttgttg ccccagcgcg ccttggtctt
     4561 ggtaatctgc gcttcgagct tgtcgaccag caggtcgatg gcttcaagcg gctgcgacgc
     4621 ggtggagttg gcctgggcgg cgatcgcgcc ggcgcggaac gtcacgtcgg ccttgtagtg
     4681 cagtttctcg cgggtcaggg tgaaatgcat ctcgtcgatt ttcgggcaga gccggcgcag
     4741 gcgattgacc ttcttgtcga tgtaggcctt ctgcgtccgg ctgatgttca tgtggcgtcc
     4801 tgtgagctgt agcggcatcc gtgaactcct ttcgctccaa gggggagctg actggcgggg
     4861 ggatgcgcct cccatccgtg gtcgggggac cgaaggttcc aggagggacg ggagggaagg
     4921 tccatgcttg gcgaacatcc cgtaaatcgg atcggcaggg gtttcctcct tctatgagtg
     4981 acgttcgcgg ggggcgacac ccgctcaggc tttgccggcc ggctcgcgcg cctggcccgg
     5041 cacctccatg tagaatcgct cgccaaggta cagccggcgg acctcggggt tgttgacgag
     5101 ttcctcggcc gccccctgca ccagaatctt gccttcgtgg atgatgtacg agcggtcggt
     5161 caccgagagc gtttcgcgca cgctgtggtc ggtgatcagg atccccagac cgcgatcgcg
     5221 catctgctgg atgatttcct gcagttcctc caccgatttg gggtcgacgc ccgagaacgg
     5281 ctcgtccagc agcatgatga tcggttcgat agccagcgcg cgggcgatct cgacgcggcg
     5341 ccgctcgccg cccgacagcg tgtaggcata gttcttcgcc agatggccaa tcgacagttc
     5401 atcgaagagc agctccaggc gggcgcgctg ctgctgccgg ctcaacttcg tcatctcgag
     5461 gatcgacatc aggttctcct cgaccgtcaa cttgcggaag atcgaggttt cctgcgtcag
     5521 atagcccagg cccgcgcgcg cgcgcttgta gagcggcatg cgcgagagat ccttgccgtc
     5581 gagcgtgatc gtcccttcgt cgggcttgac gatgccgacg accatattga acgtcgtcgt
     5641 cttgccggcg ccgttggggc cgagcagacc gacaatttcg ccttgttcga gggggatgga
     5701 gacgtgatcc acaacgcggc ggccgccgta agccttgacc aggttgtgtg tgacgagccg
     5761 atgccgcgta accaagaggg tgtgctccac gagaggtaag atcagtcaga acagggcaac
     5821 gaaccgccgc gcgctcagcg tcatggccat caactcgact atcgcccagg gcggcgggcc
     5881 gtgtcaagca agctccgtcc cttgaacatc gccgttcgtc ctcacgctac gacccgtccc
     5941 tcgtctcccg tcctcgcttc cgaaccgctg cccagtttgc cgctcattct tgaactcgtg
     6001 gtcggcccgg aagccgaggc ctgatcttgg agacaggccg gccaagctat tgattcggtc
     6061 atttgtatct ttttactgct tgtgaaaaga atcacaatgt gctaatgtct ggggaaagcc
     6121 gctaacctgg cccccgcggc ccgggtcgcc ggcctcacca cctcacttcg cctcgcgctc
     6181 tgaaaggcgg accctccgat gatgcgcaac atggattcct gggccaggga cgtgcgaacc
     6241 cagaccgaac gccaggccat gccgatcatg acctaccccg gcctgccgct ggtcggcctg
     6301 aacgtgatgg acattgtgac gagcggggag cagcaggccc gctgcatcgc ggcgctggcg
     6361 ggcgcctacc gctcgctcgc cgcgctgacg ctgatggacc tgtcggtgga agccgaggcc
     6421 ttcggctgcc cgatccagta ttcggcggat gaggtgccga cggtgaccgg cgcgatcctg
     6481 catgacctgg agcaggtcga ggccctgccg ctcccgtcgc cggaaacggg acgcacggcg
     6541 gcctatctgg aggcggcgcg gcgggccgcc gcgctgatcg agaaccggcc gctgttcggg
     6601 gggatgatcg ggccgtactc gctggcggtg cgcctgcgcg acatgagccg gttgatgctg
     6661 gacctgatgc tgcagcccga acgcgtgcac gcgctgctgg agcgcgtgac gcggtttctg
     6721 atcgcgtatg cgcgcgggtt ccgcgcggcg ggggcccatg gcctggtgat cgccgagccg
     6781 gcggcgggtc tgctctcgcc ggcgcactgc gacgaattct cctcagctta cgtcaggcgg
     6841 atcgtggcgg cggtgcagga tgaggaattt ctggtcgtgc tgcacaactg cggccggacg
     6901 cgcgagctcg tgccgtcgct tgtctcgacc ggggcgggga tgctccactt cggcaacgcc
     6961 gtcgacatgc tcgacattct gccgcaggtt ccgcgcgacg tgctcgcggc ggggaacatc
     7021 gacccctcgt cggtcttccg ccacggcaag cccgagcagg tgcggtcggc gacgctggcg
     7081 ctgctgcggc ggacggccga ttatcccaac ttcgtcctct cgtcgggctg tgatgtcccg
     7141 ccggggacgc cgctgcaaaa catcgacgcg ttttacggcg cgctggacga gtttaacggc
     7201 cggcggggtg aagcgacgtt ggccgacatc ggctgagcga aggcctttgg gagcgatcac
     7261 gaggacgagg aacgagaacg agggacgaat ctgtgcgtgg ggggttgacg cccatgcggg
     7321 gggggccgca tcatgagccg tcatgttgct gtcggtcatc atcaatacct acgagcggcc
     7381 cgaatcgctc tcgcggtgcc tgcaggcgct gacgcgccag aaggaggccg acccgtttga
     7441 agtcgtcgtc gtggacgacg gcggccggct ggacctcaag gccatcgagc gggtctacca
     7501 ggacaagctg gacctgcggc tgcggcgaat cgaacatgcc ggacgcgccg cggcgcgcaa
     7561 cgccggcatc cgctgggcgc gcggcgagcg catcctgttc ctgggcgacg acgtgatcgt
     7621 gcggccaggc tgcctggcgc gccaccggct ggaacgtgac ccggcggtgg cgcgcgtcgg
     7681 gccgttcccg ctggccgagc cgccgcgctc gccggcgctg gcgcgctggg cggaacccaa
     7741 tccgcagcac ctgatcgcgg atcccactaa tgcgggcttc ctgttctgct ccacgggcaa
     7801 cctgtcaatc gatcgggagc ggatcgtcgc gcttgggggc ttcgacgagc gtttcgcgcg
     7861 ctacgggtgg gaggacatcg acctgggctt gcggttcgag cgcgaggggg ggcggatcgt
     7921 gttcgacccg gaggcgcgcg cggtgcacga ccaccgctgg cgcgggcggg cggcgctgtg
     7981 gcggcgcgag cgcgaggcgg gttacaccgc gtttcagttc tggagcaagt ggcaggcgct
     8041 cgcgccgggg gcgatccgct cgatgaaatt ctgggatgac gcggcggcgc tgcggccggg
     8101 tccgccgtgg cgccgcgagc tgggcgactg gctggtcgcg ctgactgaaa aaatcgctcc
     8161 gcgctcggcg gtccacgcgc ggctcgtcga gcggatgatc ttcgcggcgc ggctggaggg
     8221 ggtccacgaa gcatggcgcg acgcccaggc tggcgcggca tgggcgggcg cccggaccgg
     8281 cgtgggagcg gaagcgtgag cccggaggcc gacacccgtt ccgccggcgg ggcgcggtgc
     8341 acggtcggcc tgctgacctg gaacggcggc gacgcggccg agccgtgcgt gcgcagcgtg
     8401 ctggcgcaga ccgagcgccc gctgccgatt atctggatcg acaacgcatc gaccgacgga
     8461 acggtcgaac ggctgcggcg agcgtttccg gcgcttcccg aaccgatcgt caacgcgcgc
     8521 aacatcggct tcacgggcgg gcacaaccag gcggcggccc gttgtgcgac gccgtattac
     8581 ctggccctga accaggacgt cgtgctggcg ccggaatacg tcgagcgggt ctgcgcctgg
     8641 ctggacgagg cgccggacct ggcgctcgcc tcggggttga tcctgcgggg cgccggtccg
     8701 ccgggcgagc aggcgccgac ggcgccgggc ccggatgacc ggatcgcctc ggcggggatc
     8761 gtctggccgc ggctgcgatt tccgttcgag ctggcgttgg gatggccggt ggaggaggtc
     8821 ctgcgaacgc ggcgctgggt cccgggcgtc aatggggccg cgatgatcct gcggctggcg
     8881 gcgtgccgcg ccgcggcgat cgagccgcag cccgaccccg ccggggggga gccgctgccg
     8941 gggatcctgc cgcccgattt tttcgcctac cacgaggagt tcgacctggc gctcaagctg
     9001 gcccggatgg gctggcgcgc gggggtcgaa gggcgggcgt acgcctggca cggccggttc
     9061 agttcggggg ggctgcggcg gcggtcgatc cgggcccgcg cgctgctcaa ccactggctg
     9121 ttgacgctgc gccacgacga ctggccgacg atcctgaagg aattgccgta tctgctcaag
     9181 ggggagctgc aattctggtt gccgcgctat ctcatgtatc ccggggcgtt cctggaggcc
     9241 gtgggcggac tggcgcgtgg ggcggcggcg gcgcggcgct tccatcgccg gttcacggcg
     9301 catgcggcgg cggcggaccg gccggatcgg cttgcggaat atcgccggga aagcttgcgg
     9361 caactgcgcc agtggaaacc gcgccggacc gttgcggaaa agctgcgccg gaccggcgga
     9421 tcgttttgag attgcttcca ccgcgctcct cgcggggctg tccagacagc tcccggcctg
     9481 ggcgcaggcg gattggcagg gcgggtcagg ccgattcgcg tttttcggac tggatgaaca
     9541 acggttcctt ctcgccgcgg atgacgccgc cgtcgatgtg gcactcgtcg tattcctcgg
     9601 ctttttcggg gagcgaaaac atgatgtcga gcatgatctc ctcgaggatg gcgcgcaggc
     9661 cgcgggcccc cgtgcgcagt tccatcgcct tgtgggcaat ctcgtgcagc gcctcgtcgg
     9721 agaagtgcag ctcgaccgag tccatctcga aaagtttctg atactgcttg atgatcgagt
     9781 tgcgcggcgt cgtcagaatc tggatcagtt ccggttcatc aagctggtcc agcgtgcaga
     9841 cgatcgggaa gcggccgagg aactcgggga tcatgccgaa cttgagcagg tcgtagtagt
     9901 tgacctgctc aaggatctcg ccgatgcgat aatccttacg gctgcggacc tcggcgccga
     9961 aacccatcgt tttctgctgg acgcgctgtt cgatgatttt gtccagcccg ctgaacgcgc
    10021 cgccgcagat aaacagaatg ttgcgcgtgt cgatctggat gtattcctga tgcgggtgct
    10081 tgcggccgcc ggtcggcggc acgttggcga cggtcccttc caggatcttg agcagggcct
    10141 gctggacccc ctcgcccgag acgtcgcggg tgatcgaaac gttttgcgtc gtgcgggcga
    10201 tcttgtcgat ctcgtcgatg tagacaatgc cgcgttcggc tttctccttg tcgccgccgg
    10261 cgttctgtag caggcgcagc aggatgttct cgacatcctc gcccacgtag ccggcctggg
    10321 tcagcgtcgt ggcgtcggcg atggcgaagg gaacctcgag gatgcgggcg agggtctgcg
    10381 cgatgagcgt cttgcccgag ccggtgggcc cgaccatcag gatgttgctc ttctccagct
    10441 cgatatcctg gctggccagg ttgtgcatca ggcgcttgta atggttatag acggcgacgg
    10501 agacggtctt cttggttttt tcctggctga tgacgtattg gtcgatcgcg cttttgattt
    10561 cggaggggga agggagagtc ttgagggttt cgaacgccgc ttcctcggcc tgcaggatct
    10621 gatcgcgctt gaggcgttcg tggcactggc cgacgcactc gtcgcagatg taaaccgtca
    10681 tcatgctctc cttctgggtc agacccttga agagcgtgcg gacggaatgc tggtccttgc
    10741 cgcagaacga gcaacgaagc ttcatggttg ataccaccga tgggtgaact caggcggtat
    10801 ccgcgggaac ccgcctgccg tggcctttat cctaatctaa agcggggacc ggctgcaatc
    10861 cacgggtgcc atttttttgg tggtcgaggt tgccgggcgg cgggggtggg ggcggcgggt
    10921 agcctgatgc ccgctgggga tagaatggcg ggttttcgag agccgaggac gagggacgag
    10981 agccgggagc cgaattccgg gagggccttc gggggtctgg aagccggtct ttgcaacgct
    11041 gagctggaat ccgcttacga tccagctacc gatgcccagg ccgacagcga gaccgggaat
    11101 tcacaaacat cggcttgatt ccgtgccgtg gggtggtatg ctgggagcaa cgtttgtgcg
    11161 cggaaaggtc ggcgccgcga tgcgcttgaa ttcaccaatt cccctccgcc ttcctgtcgt
    11221 tgcggcgatg gtgagcgact gacgccggaa gatctcttcc tcccgccggc ccgctccccc
    11281 tgacaggcag cgggcctccc tgttttcacc ccatcattca ggtccgctgc ttgttatccg
    11341 gctcgcgcgc ccgcgactga ttcgcggttc tccatcccgt cctgatgggt gcaggtcaag
    11401 gacgcctccg atgagtgcca cttcgccgac ggtctatcgc tcgctgtggt tctggatgcg
    11461 cggccaccgt ctgcgctacg gcgcggccgt gctggccctg ctgctgtccg tttatttttc
    11521 gtttcaggtc ccgctggtca tcgccgacgt gatcgacgcg atcttcaggg agggggcggc
    11581 cgaccgcgag ccgagtcggt tcgtgaaact cctgctgcgc gtcgtggggt cggacggcct
    11641 gcgccggcac ctctggctgc ccgggctgct gatggtcggg ctgatgctcg tcgccggggg
    11701 gttcagctac ctgcgcggcc tgtggatcgc ccaggccagc gaatcaatca tccgggcgat
    11761 gcgcgagcgg atctacgatc accttcagca tctgcccgtc gcgttccatg ccgccgccga
    11821 cacgggcgac ctggtccagc gctccacctc cgacgtcgaa accgtgcgca actttctggg
    11881 cgggcaggtc accgaactgg ggcgcggcat catcatgctg atcgtgctga tcccgatcat
    11941 gctcacgatc aactgggtga tgacgctata tgcgctctcg ctgacgccgc tgattatcct
    12001 ggcctcgctg ttcttcttca gccgcatcaa ggcggcattc aaggagcagg acgaagcgga
    12061 ggccgcgatg acgaccgtcc tgcaggagaa cctgaccggg attcgcgtcg tccgcgcgtt
    12121 tgcccgccag gagcatgaga tcgccaagtt ccaggggaag aatgccgcct accgcgacct
    12181 gcagtaccgc gtggtgtgcc tgatggcgtg gtactggccg tcgtcggacc tgatgtgcct
    12241 ggcgcagacg gggtttgtgc tggtcgtcgg cgcgcgcttc gtccagcacg atgcgctctc
    12301 ggtgggtcag ctcttcttct acatggcctg cctgggcatg ctgctgtggg gcccgctgcg
    12361 caacatgggc cgggttctgg tcgacatggg caaagcgcag gtcgcgatcg ggcggatcga
    12421 ggaaatcctg cgcgaaccgc gcgaatcggg gccggcgcgg ccgatggcgt tgccgaccga
    12481 aatgcgcggc gagatcgagt tcgaccgcgt ctcgttcgcg tatcaggatg ggcagccggt
    12541 gctgcacgag gtctcgttcc gggtcgagcc gggccagacg tttgccattc tcgggcccac
    12601 gggatcgggc aagtcgacgc tcgtcagcct gttgctgcgg ttccacgatc cagcggcggg
    12661 ggcgatccgc atcgatggcg tcgatatcgg ccaggccgag cgcaaggccg tgcgcgcgcg
    12721 gatcggcgtc gtcctgcagg agccgttcct ctattcgaag acgctgcgcg acaacatccg
    12781 cttcggccac agcgaggcgc cgcgcgaacg gatcgaggcg gccgcgatcg ccgcctgcgt
    12841 gcacgacaac atcctcgatt tcgagaaggg ctacgagacc gagatcggcg agcgcggcgt
    12901 gacgctgtcg ggcggccagc gccagcgcgt cgcgctggcc cgcgcgatcg tcaaggatcc
    12961 gccgatcctg atcctggacg acgcgctgag cgcggtggac acccacaccg aacggctgat
    13021 cctgcgggcg ctggccgacc gccatgggcg gaagaccacg atcctcatcg cgcaccgcct
    13081 ctcgacgctg atgcacgccg accggatcct cgtgctggat cacggccgcg tgattcaaac
    13141 cggttcgcac gccgaactgg tcaaccagga gggactctac cgtcggttgt ggagcatcca
    13201 gacgtcgctg cagcaggatc tcgccggcga cccgcccgcc ccggccgccg aggcgcgggc
    13261 cgctcttgca caagcggagg cgccgacgca tgtctgagac cgtgttccgc gaagaggaat
    13321 tcaacaaacg gctcgatccg cggctctggt ggcggattct gcagttcgcc cgaccgtacc
    13381 ggccgctggt gggcgggctg atgacgatcg cgctgctcgt cggcctcacc gaatcgctct
    13441 acaatctgct ggtcaagcgc gtgatcgacg gtctggtgga gttcggcccc gacgcccccc
    13501 tgttcagtta cgcgctgcag tacgccgcca tcacgatcac gctggtggtc tgcgttttca
    13561 tctttatccg tttcgccggc aagctttccg ctttcatcag ccacgacatc cgccgggccg
    13621 gtttcgcccg cctgcaggag ctctccttct cattctacga tcatcggccg gtcggctggt
    13681 tgatggcccg tatgacgtcg gactgcgagc ggctttccag cgtgctggcg tggggcctgc
    13741 tcgatctgac ctggggcacg gcgttcatgt ccgccatcgc ggtgatcctg ttctatctga
    13801 attggaagct ggcgctgatc gtgtttacgg tcgtgccggt gctggcctgg atcagcctgc
    13861 atttccagcg cgtcatcctg cgcagctcgc gcctggtgcg gaagatcaac tcgcgcctga
    13921 ccgggtcgtt caatgagggg atcagcgcgg tgcgcacaac caagacgctg gtgcgggagg
    13981 aggggagcct gcgcgagttt gcaagcctgg ccggcgagat gcggcgcgcg tcgatccgca
    14041 atgccgtcca gtcggcgctc tatttcccgc tggtcatcac gatcggcagc gcgggggccg
    14101 ggctggcgat ctggttcggc ggacgcgacg tgctggccgg cgtcatctcg ctcgggacgc
    14161 tggtggcgtt cgtcagctac tcacgcgatt tcttcatgcc ggtgcaggaa ctggcgcgcc
    14221 tgttcaccaa cctgcagatg gcgcaggcct cggccgaacg gatcatcggc ctggtcgata
    14281 cggagccgga aattcgcgac agcgccgagg tgcgcacgtc gatcgcccgc gccggcgctc
    14341 agccgccggt cgccggccgc gcgatcgacg gcttgcccga ccgcatcgag acgatcgaat
    14401 tccgcggcgt ctcgttcgcc tataacgtac gcaagccgat gggggccgac gacgcctcgg
    14461 ccggcgaacg ggaggtcatc aaggttcacc cggtgctgga tgacttcaac ttccaggtgc
    14521 aggcggggca gacgatcgcg ctggtcggtc cgaccggcgg cggcaagtcg acgatcatca
    14581 gcctgctgtg ccggttttat gagccgaccg ccggcgagat cctgatcaac gggatcgatt
    14641 accgccgccg cagcctgcac tggctgcaat ccaatctggg gatcgtcctg caatcaccac
    14701 atctgttcag cgggacgatc cgcgagaaca tcgcctatgg ccggctggag gccagcgacg
    14761 aggagatcgt gcgggcggcc cagctggtca acgcccacga gttcatcatg gcgatgcccg
    14821 ggggctacgg ggcacaggtc ggcgaagggg gcgtcaacct ctccaccggc cagaagcagc
    14881 tcatctcgtt cgcgcgcgcg atcctggccg atccgcaggt gttcgtgatg gacgaggcca
    14941 cgtcgtcgat cgacaccgaa accgagcggt tgatccaggc cgggatcgaa cgggtgctga
    15001 gcaaccgcac gagcttcgtc atcgcgcacc ggctctccac gatccgccga gccgactgca
    15061 ttctggtgat cgaaaaaggc cggatcgccg agcagggaac gcacgagcaa ctgctcgcgg
    15121 cgcgcggaca ttatcatgat ctttacaccc atcagttcac ccgcgaggcc gaggaacagg
    15181 tgctggccga tccgctgttg gcctgatccg ggggaaggcg cgcaggggga gccgtccgtt
    15241 ctcgccggcc gctccggccg ccccgttcag ggccttggtg ggtccggttg atcgagtcga
    15301 aaccggccgg tttgggccgg caacaaagga ttgcttgaaa cgccgcgcag gcgcggtaga
    15361 atgttgcccg tttatccgac gctcacggcc ggctgagcca cggcgcgacg tcgcccatcc
    15421 cccccaccga ggtatttccg ttcgtgggcc tgatcgttca gaaattcggc ggctcgtcgg
    15481 tcgccgacgt cgacaagatg aaactggtcg ccggccgcgt catcgccgaa tgcgaccgcg
    15541 gcaatcaggt ggtcgtcgtc gtctccgcga tgggcaagac gaccgacaac ctgctcagcc
    15601 tgatcgcgca ggtgaccgac cgccccaacc cccgtgaagt ggacatggtc ctggccaccg
    15661 gcgagcagat ctcgatcgcg atcctggcgc tggtcctgca gacgatgggg cgcaaggcga
    15721 tttcgatgac cggcccgcag gtggggatca tcaccgaccc gtttcatcgc aaggcgcgca
    15781 tccgcgacat caacacggcg cggctccggg agaaactcga cgaggggcac atcgtcatcg
    15841 tcgccggctt tcaggggctg acccagtccg ggcagatcac gacgctgggg cgcggcgggt
    15901 ccgacacgac cgccgtggcg ctggccgccg cgctgcacgc cgaccgctgc gacatctata
    15961 ccgacgtcga cggggtctac acgaccgacc cgcggatcgt ccccgacgcg cgcaagctcg
    16021 ccacgatcac gttcgatgag atgctcgagc tggcgagcct gggcgccaag gtcctgcaca
    16081 accggtcggt cgaactggcc cgcaatcaca acgtcccgtt gcaggtgctg agcaccttct
    16141 cccagaaccc cggcacgctg gtcgtcaagg agtatacgaa catggaagac atcgtcgtca
    16201 gcggcgtcgc ctacaaccgc gacgaataca agatcacgat tctcggcgtg ccggaccagc
    16261 ccggcatggc ggccaagctg ttcagcaaga tcggcgaggc gaacgtcaat atcgacatca
    16321 tcgtgcagaa catcagccac cggggtctga gcgatatctc gttcacggtc ggcaaggagg
    16381 atttccagaa cgcgcgcgac gtcgccgagc agttcgtgaa ggaatacggc gccgagggga
    16441 tccgcaccga cgacgcagtg ggcaaggtgt cgatcgtggg ggtggggatg cgcagtcacg
    16501 gcggcattgc cgcgacgatg ttcggcgcgc tggccaagga aaacatcaac atccagatga
    16561 tctcgacctc ggagatcaag atttcggtcg tcatcgcccg cgccgacgtc gatcgcgccg
    16621 tgcaggcgat ccatcgcgaa ttcgaactgc agagcgaacc gaaactgacc gcgcgttgat
    16681 gccctagatc gcgcgcagca tgccgccggc gcgcgcgcgt ttgcgttgca ggccgcggta
    16741 ggcctgcagc acatgctcac ggatttcttc cgccgtccgc agcgaataga cttcctcgac
    16801 cagtcgccgc gcgtcggtca actcgacctg gcggatcagg cgcttaatct ccggaatcgc
    16861 cagcgcgccc attgacagct cgtcgatccc catcccgagc agcagcagcg cgcagagcgg
    16921 gttggccgac atctcaccgc acaggccgac ccagattccc tcgcgctgcg ccgcgtcgat
    16981 cgtgttgcgg atcagccgca ggatcgccgg gtggaagggt tcatacaggt gcgccacctg
    17041 ttcgttgccg cggtccacgg ccagcgtgta ctggatcaga tcgttggtgc cgatggagaa
    17101 gaaatcgacc tcgcgggcca gttggtccgc cgtgatcgcc gccgagggga tttcgatcat
    17161 cacgccgatc tggatttcct cgtcgaacgg gatccgctcg cggcgcaggt cggcggccac
    17221 ctcgtcgagc atctcgcgcg cctggcgcac ctcggcgacg ttggagatca tcgggaacat
    17281 gatgcgcgcg ctgccatggg ccgaggcgcg caggatcgcg cgcaactgcg tccggaaaac
    17341 ctccggatgc gccaggcaga gccgaatcgc ccgcaggccg aggaacggat tgagctcccg
    17401 ccccgtctgc agcgccccca ggaatttgtc cccgcccagg tcgatcgtcc ggaagatcac
    17461 cggatgcggg tccatcacct ccacgacctt gcggtagacc tcgaagagtt cctcctcgcc
    17521 cggcaggcgg tcgttttcca gatattgaaa ctcggtccgg aagagcccga tcccctcggc
    17581 gccgtgggcc agaacgtggg ggatctcgat cggcagctcg acgttcgccg ccagatccac
    17641 ccggtagccg tcaatcgtct cggcggggag gtgcttgagc ttctgcaggt agcgttcgtg
    17701 gacgaggtgc ttcttgcgcc gctggcgggc gcgcgcgatc tcgcgcggcg acgggtcgat
    17761 cgtgatcgtc ccctcatagc cgtcgataat cgtctgcgtg ccggtcttca acccggcgag
    17821 gaacggcccc agcccgacga cggccgggat ttccagcgcc ttggccagga tcgccgtgtg
    17881 cgacgtcggc ccgcccagct ccgtggcgaa ccccttgacg tgcaactggt ccatctgcgc
    17941 cgtgtccgac gggctcaggt cgcgcgccag cacgatcacc tcgctgtcga actgcagggc
    18001 gctggagctt tccggctgca cctgcgcgag gtgctggcag acccggttgc cgacatccag
    18061 aatgtcggaa ttgcgcgagg ccaggtaggg gtcgccgacc gaggagaact tgtcgacgaa
    18121 ctggcggatg atcgtcatga acgcgcgcga cgcgttctcc tggttcttct cgatgcgctc
    18181 gaccgcgctg tcgacgacgg cgggatcttc caggatcatc aactgcgggt cgaagatcga
    18241 ggcgtgcgct tcgtccagcg tgtcgcgcac gcgttcgcgc aagcgctgca gttcctcctt
    18301 ggccttggcc agcgcctcgc gcagatgctt cttttcattt tcgatcgcgc gggccgacac
    18361 caggtggtgc ggcaccaccg tctgcacggg atcgtagaca tggatcttcc cgatgtagat
    18421 cccgggcgag accgcgattc cctggtaggt gcgtgaggcg ggcttgggtg ccgtgggggt
    18481 catgaaggga gcattatagg gataaacgga caaaaagaaa agccggaccg cgcaggcggt
    18541 ggggggaggg agacgagttc aaacgggggc gcgccggcgg cggcgcctca ggtctcaccg
    18601 aagccgctct cgatcagcgc aaccagttcg cggatggctt cttcggaccc ttccccattg
    18661 gcttccagga tcaactcgct gccctgctcg gccgcgagca tgatcacgcc catgatgctc
    18721 ttggcgttga tcgactggcc gtccttggtc aggaagacgt cgcacttttc gtatttgctg
    18781 gcaattttga ccagttgcga agctggccgc aggtgcagcc ccagcgaatt ggggatggtc
    18841 acggtggctc gatgaccggt ctgtgtttgc tccatcatga acgcgctcgg tgctaggttt
    18901 ggacccctgc ggcggctgat ctcggcgatg gcgcgggggt cacgctgcgc gggttcggcc
    18961 tggtcaaggg ggaggaaaag atgactggcg aaggcgtagc cgcggatccc ccggaccgcg
    19021 ggcacaacat ccttccgtta ggatagaatc catcgtccga aagtcaaggg ctcattggga
    19081 acggcgggcg gccggccgac gggcagaccg gtcgcgatgc ggcgccatga acgggttaac
    19141 tcaaataaat acagtgcttc gtgttttgcg aaggaagcca tcaggcgcac cggggggcgc
    19201 cgcgccggtc tcagcggccg aacggcgcgt cgtccccgga tgcctcctca tcgggcgaaa
    19261 ccggacgaga tttcccggcg tccgcctcgc gcgcgtcggc ctgctgatgc tgcgcctcac
    19321 gctcctcgaa attccgtaga attcccaatc gtcgctcgcg cccgatctcg cgctgcagga
    19381 tcagcgcgcc cgcccccatc atcgccgccc acaccagcat cgtctggcga taacccagcc
    19441 agctccccag cgtgtaggca atgaagggca tcgtcacccc ccgcatcccg gtcagcgact
    19501 ggtgcagccc catgtaaagg gcgatgtcgc gcgagcgggc gtagtaattc acccccagaa
    19561 tccagatcag catgccgccg ccctgcatca gcccggtcac cagcacgccg ccgtagatcg
    19621 ccggcatcga ccgcgagaag taatagatga tcggcacgag catcagcagc acgttgatcc
    19681 ggaatcgcat caccagcggg ttgttccggt cgagccacat cccccagacc gggagcatga
    19741 ggaaggtaat cagcgtcggc aaggtctgca gggcggtggc ggcctgctcg taactggcgt
    19801 tgaactcggc tttcaaatag agcgggaaga tcgtcgcgag cgccagattg cctgctccga
    19861 agagcatgaa cgagacctcg aaattacgga aaccccggtc gcgcccgagg atgcagaact
    19921 gctcccaaag ggagacgcgg gtcgtccctg gctcgggccg ggcggccgcg gccgcctcct
    19981 tgcgcaaacg cagccggctg aaattccaca gcccgaccaa tccgatgccc cccgccaccg
    20041 catacagcgg tccgagccac tgctcggagt gctcgagcgc ctgccccgcc agaaacgaat
    20101 aggctccgat cgcgagcatc tgcgcggaca ccagcatcga catgacccag gagcggatcg
    20161 ccggccggta gttcgtcttg atcagggccg agagcgcgct gaccaccggc gcccggctca
    20221 actgcatcag catcacgatc acggcgaaat aggcgtccat cgacgggcgg acgggcagca
    20281 gcagaaacga caggcagaac gcgatgcagt tgggccagag cacccagccc aatttgtcgg
    20341 agcgctgcac ccgattggaa aagacgaccg ccaggatgtt gccggcatgg aacgtcgccg
    20401 ccagcaccgg gacccagtaa tcggtctgga acgtcttggt catcaggaac ggggcgtagg
    20461 tcaccatcat cagttgcggc gcgctggaca gggccaggac gaagtgcgcc ggcaacgtct
    20521 ggcgccccga cggcgagagc tggcgcagac gatctgtgat gaaggaaacc aagttgatgc
    20581 gccctgggga cgccggttcc atcccccacg gtgacgagtc ggtttggccg tgattaaacc
    20641 atgccgcccg cccgattgtc aaaagccggc gggcgggttg gcggggcggt cgaaaccgcc
    20701 gtgcgggggc ggcgagggac gccatgacgc acagggagtg gggaattcca atggggagtc
    20761 tttgtcaatt gccaatacca agcatcgttc gagcttcttg aattaaattc gatattgttt
    20821 cgaattccga gattcgtatt tcgaattttc gtcctccccc tcttgctgtc ctgctccatt
    20881 ctttccaaac ttcttcgccg atttccgccg ttcgcgctat catggcgcgg tcgggcatcc
    20941 acgttgcgga ccctgccgcc cgcaaatcca gattaccccg ggggaaaccc acatgttcca
    21001 gcctccgcac cgcttcatcg gggccatcct gctcgtcgtc gcgctcggcg gcgcggcggc
    21061 ctgccagcgc gcctactaca gcgtgatgga acgcttcggc gtccccaagc gggagattct
    21121 ggtcaatcgc gtcgtcgagg cgcgcgacgc gcaggaggaa gctcgggagc ggtttcagtc
    21181 cgcgctggag cgattccaga cggtcctggg ctttgacggc ggcgagctgg aggataaata
    21241 caacgcgctc aacgccgagc tcaaacgcag cgaatcgcgc gcgagcgcgg tgcatgatcg
    21301 gatcgatgcg gtgcgcgacg tggccggcgc cctgttcgcc gagtgggagg atgaactgga
    21361 ccaatatgag aacgccgacc tgcgccgccg cagcgaacgc cagttgaagg agacccgcga
    21421 ccgctatcag caattgatca cggcgatgga gcgggccgaa tcgcggatcg agcccgttct
    21481 caagccgctg cgcgatcagg tcctgttcct caagcacaac ctcaacgcgc gcgcgatcgc
    21541 ctcgctgcag gaggacctgg gcgagattca gaccgacgtc gacgcgctga tccgcgaact
    21601 ggagcgcgcg atcgccgagg ccgacgcgtt catccagcag atggagacgg ccgagtaaag
    21661 gtcatcgccg cggggtcggc gggcgaacgc agcgccctgg ggagaacgcg aacgatgacg
    21721 cgccgcgcgt cctgggaact cgacccccgc gatctgccgc cgcgccagct acgcgcgcga
    21781 gccgtcgctt ggcaaccccc ccgagaatgc cttttaccgc ctcagcacac tgcagcgcga
    21841 gcgcgtgcgc tgggcgacgt atcatggcgc gaccccctgg ctcctgctgg tggtcctcgc
    21901 cctcctctgc aatgtggtgg ggatgattca cttgatcggc ctgcggcttg tctcgctgcg
    21961 caaattgaat ctgaccggcg agccgcaact gttcgggctg cgccgcgccg tgctgaacga
    22021 cctgctgctg accgagctga ccccgcacga tttcgcggtc ggctactggg gccagcaact
    22081 gaacggtcgg gcggtccggc ggagccgggt catcctcggc gtttcgcagt ttgtcacggg
    22141 atcggtgttt ctcggcgtat tgctgctggc gccgcccgac ggcggccgcg aggtctggct
    22201 ggaatgcatc agcgtcgccg cggcaatcgt cgggtggcag gccggcgtcg cgcggtatga
    22261 accgtggagc agcctgccgc gcctgacccg ccgcgtgcag cagcagcggc ggctgttcgg
    22321 cacgacgcag cgcgagcgga tcaccagcct gctcgcctcg gaagcggcga tctacattgc
    22381 gctgctggcg gtcgggctgt tcgtcctgcc gcacgtgctg ctggtgctgg ccgggatcct
    22441 ggtcggcgcc aaactgcatg tgctgctgac ccacccgctc ggaccgccgg cgtgggcggt
    22501 tgccggcgag ctgctcggga tgcgcgaggg ttggcgccgg gccaagtcgg cgccgcggct
    22561 gctgcaacgc atcgaatggg attttcatta cattttctat tttcatcggt ggggcgagga
    22621 cgaggtcgcc gcccgcgagg cgccggggtc cgcgcccgcc cccgccgcgc gcgccgaatc
    22681 agttcccgac cgctaccgcg cgccaaaatc gccgattcgc gccgacgccc agactccgcc
    22741 cgaaaaatcc gaagcctgaa tgaggaaggg gtccgcccgc gctactcgct ctcttcgtcg
    22801 aggatcagcg ccggcgcggg atgatccgga tggcgaatcg attcgaccat ctcgcggatc
    22861 gcccgcggcg gggcggcggt cagtcgcgac acgacgagtg tcaccaccac gttgagcacc
    22921 atgccgactg cgccgatgcc ctcggccgag attccgaaga accagttgcc gaaccatggc
    22981 tggtccgtgc cgaagatctt gtgcgcctgc gtcccggtga tgtagatcac cacgaacgag
    23041 agcccgacga tcatccccca gatggccccg gtccggttcg tgcgcgaatc gaagatgccc
    23101 agaatgatga tcgggaagaa gctggccgcc gccaggccga acgcgaatgc gacgacctgc
    23161 gccacgaatc ccatcggatt gatgccgaag tagccggcga cgatgaccgc caggacgacg
    23221 cagatgcggc cgaccatcac gcgctgcccc tcacttgccg tcggccggat cagtcggtag
    23281 tagatgtcgt gtgcgatcga gctggagatg accagcagca ggccggaggc ggtcgagagg
    23341 gccgcggcca ggccgccggc cgcgaccagc gcgatgaccc aggcgggcag ccgcgcgact
    23401 tccggcgtcg acaggacgat gatatcggcc gagaagtaaa cttcattggc cgtggccgga
    23461 ttcggctgcg cgaggttcag atcgccgtcg gtgcgcagga aggcgtcgcc cggcgcgatc
    23521 tgaatccgcc cgtcgccgtt cttgtcgacg tatttcagga gctgcgtctt ttcccagctc
    23581 gccacccact cgagctgctc gatctcctcg atcgtcttgc cgttgagcga ggccagcagg
    23641 ttataacggg caaacgtggc gagggccggc gccgtcgtgt agagcagcgc gatgaagagc
    23701 agcgcccagc cggcgctcca acgcgccgcc cggacgttgg gcaccgtgta gaaccgcacg
    23761 atgacgtgcg gcagcccggc cgtgccgacc atcagcgcca ccgtgatgca caacacgtct
    23821 agcagcgact tgtcgcggaa cggttcggtg tattccttga aaccgagatc cacctggatc
    23881 tgattgagtt tttcggtcag atcgctgaag acgaacgcga gctgcggaat cggctggccg
    23941 gtcagtttga gcgccagcgc cgaggaggga atcaggaacg cgaggatcag cacgccgtat
    24001 tgcgccacct gcgtccaggt gatccccttc atgccgccga gcacggcgaa gaacgcgacg
    24061 atgaccatgc cgatcacgac gccgacattg atcgggactt cgaggaaccg gctgaagacg
    24121 atgccgacgc cgcgcatctg accggcaaca taggtcatcg agatgaacag cgccgcaatc
    24181 accgccacca cgcgcgcggt atcggagtaa tagcggtcgc cgacaaaatc ggggacggta
    24241 tacttgccga atttgcgcag atagggcgcc agcagcaggg ccagcaggac gtagccgccg
    24301 gtccagccca tcagatagat cgagccgtcg tagcccatga ccgaaatcag gccggccatc
    24361 gagataaacg aggcggcgct catccagtcg gccgccgtgg cggcgccgtt ggcgatggcc
    24421 ggcacgccgc ggccggcgac gtagaagccc gaactctcgc gcacccgcgc ccaccagcca
    24481 atgtagaggt ataacatgaa ggtgacggtg acgaggacgt aggtccacac ctgaacggcg
    24541 gaaaggtcgg tcatggcact cccctggatg gcgtcgggcg gcggtccggc cccggctggt
    24601 cagtgtgcct ggggcggcgg cgtgggatcg gcgtccacat cgaggcggaa cttgtgatcg
    24661 agccggtcca tccgccaggc gtaaaagaag atgagcagga cgaagacgta gattgagccc
    24721 tgctgggcga agaagaaccc aagcggcagg ccgccgagct ggatcgcatt aagctgacgg
    24781 acgaacagga tgccggcgcc gagcgagacg agggcccaga tcgccagcag cgtccagatc
    24841 aggcgctgat tggcgcgcca gtaggggtca agcaggtgac ggtgcttgtc gttgggcgac
    24901 ggggtggagg tctcggatcg aggattggcc atgcagatcg ctcctggcgc atccggcggg
    24961 cggcgcgtga actgggcgag gtggatcagg tcgtgcggtt gccgggccgg ggcgcgtgag
    25021 atcagagcgg acgggccggg gaccgcggcg cctggaaatg attccccata ttgaggtcag
    25081 gattctagcc cgcccgcccg aaaaggcaac gagtttcttg ggtatccgcg aactaacggc
    25141 aggttgagat ctagaatgcg ctctgcaggc tgaaatggaa gaagcgcgtc tcgtcgtcgc
    25201 cgtggctgac gatcggcgcc gccagatcaa gccccacctg gacattgcgc atgtcgaagc
    25261 gcagaccggt gccgagcgac acgcgcggca cctgccaact gaccggactg tcgcccagcg
    25321 tcccgacatc cacaaagagc agccccgaaa tcggatcgac gatcgggtaa tgcagttcgt
    25381 ttttcacgag gattttggcc ccgccgccga ccgggatgtc gtcgtcgtcg ccgtcgaaat
    25441 acccggcgcc gcggtaggca aagccgcgca tgtcggtgtt gccgcccagg aaatagcgtt
    25501 cgtgaatcgg gacgaggtcg gccgaaaacg gcatcaagcc ggccatcatc gccaggcgcc
    25561 acgtcatccg ctcgcggacg gggcgatatt gatcgcgctc ggcttcgagc ttgaccagcg
    25621 gtccgccggc atagccgatc tcggcgccgg ccgcctggat gtagccctca cgcgggcgct
    25681 cgccgaccgg ctcgcgcgta tcctcgatga atttgagccg ccccgtcagc accgcgtacg
    25741 atcgatccag ctcgtcctcc aggtcgtcgt cgtgctcgcg ctcgttcagg tcgacgccct
    25801 cgagccggcc gcggacatac atcgtccagt cgctcgcttc gagcgggtgc gcccattcgg
    25861 tgtgaaaccc ggcgacgttg gcgcgatagc ccggccggcc catgctctcg tagaacagcc
    25921 ggtaggccat cgagtcgcgc gtttccttga aatggcggtc cagataggtg atcgacagtt
    25981 cgctttcgcg cgtgccgagc cggccgttga agccgaagac gtcggcgcgg ccgccgacgt
    26041 tgcgctcgtt gaacgccgcg taaacgaacg cgccggcggc atcgccgaat ccgacgccgc
    26101 cgctgatcga gccggtggtc gactcgcgca ggctgagcac caggtcgtgg acgcccggtt
    26161 cggcggtcgc ctcattgcgc gcgttgacgt tctcgaagat cccccagcgg ctcagccggc
    26221 ggaccgtttc gcgttcgagg cgcttgttgt aaacctcgcc cggctccagc aggacttccc
    26281 gcaggatgac cgagtttttc accgggggcg ccatccggtc gtaccagccg cggaaccagc
    26341 ttggttcctc gtcctcggcc aacgggggac gctcgacccg gacgcggccg acgtaaatcc
    26401 gcgacttctc gtcgacgtcg atcaccatgt tcaggacctg gttttcagtg tcgtactgat
    26461 attcgggggt gatctcggtc gtcagcaggc cgtggttgta gtacaacgcg ctcagttcat
    26521 cgagcgcctt ggcgaaccgg cgggcatcga acagttcgcc cttgagcggc tcgaacgggg
    26581 cggcgacctc ggcatggctg aacaggcgcg cgccgcggac gcgcgcgtcg ccgaacgtgt
    26641 agcgcacgcc ctcgttgacc tgaatcacgg gcgagacgac cggaatcttg ccgccggtgc
    26701 gttcgacgaa catcccgcgc tgcacgaccg catcaaagta accgtgcgcg gcgtaaaaat
    26761 cgcgcagccg gtccagatcc tccgcgaacg tttcctcatc atagtaattc ttgacgatca
    26821 ggaccgagcc cttggtctgg agcatcccct tgaggcggaa gtcgctgaag gcgtcattgc
    26881 cctcgatcag cacgtcgtcg acgaggacct ggcgcccctc gtcgaggacg atctgcagat
    26941 ccgcctgcgg gatcggcgcg ccgtcgccgg tctgctcggc cggctggtcg atcagattga
    27001 gcgtgacgcg cgcctgcggc atgccgagcg ccttgtattc gttgcgcaac gcgcggcgaa
    27061 tgcgatcgag cgtcgcggcg tcgagcaggc tgccccgctc catcccgacc agccgttcaa
    27121 tccgttcggt cttaagcttc tgattgccga cgacttgcag ttcgcggaag cgggggaatt
    27181 cgctgacggt aaagacgacg tccagaccac cgccgggcgt cgtctcggtc tggacattga
    27241 ccgtgcgaaa gatcttgccc agttcctcga tgtcctcgcg catctcgacc ggatcgtaaa
    27301 cctggccggg ctgagtctgg aggcggggga gaatctgctc ggggcggtag ccggccaggc
    27361 cgtcgatccg caccgcgtcg acgcgcgcgc cctcgccctg cgccgaggcg gcggccgcgg
    27421 tcatgatcgc gagcgtccag ccgaacaaca gcgcccggaa gggcaggcgg ggagggcagc
    27481 ggcggcgggc gctcgcgcgg cggaactcag acatgcaagg ccactttcct caagcgttgg
    27541 aaccggccag gaacggcggt cccgggcatg cggcgcacaa gccaaatggt ttacttgccc
    27601 ccgccggtgc ttgcaaggga aaacgccgac aacgctgcgc tgatcgagcc cctggcggtc
    27661 ataaaatcgg gataacgcaa cgcatggtgg ggatgacgaa acgcacgatt ggacacggag
    27721 cgggaatggc gagcgacgcc tgactgccaa cttcttcctg cttcccctgc ggcgccttct
    27781 ctccctaaat tccttccgtt cctcgtccca gcctcaatcg gcttgccaac ggtccccgcc
    27841 gtgcaataat cggaggacct gaccgcgctc cggcaatccg ccccctggga accgcgcatg
    27901 gctcgtttgc ttgtcgccgg catcggcccg ttgccccccg aacaacccgg ccggctcttt
    27961 gctccggggc tgaggatctg gggcattgcg cgggaactgg cgcgcgccgg ccacgccgtc
    28021 cggctggtgc ggggccggtt cggcgatggc gccgccggcg aggccgcgct gttcgacctg
    28081 gagcccgccg ccggcgcgcc ccgcggcccg gcggaactcc cgccgatgcg cacccgtcgc
    28141 gtcgccgaag ggggctggcc gcaactgctg gcccacgagg cgacgcagtt caatgcgacg
    28201 gccgccgtcg gttccaccga cgtcatgaac catgccctgg cggccaccgg cttgccgctg
    28261 ccgatctgga tggattactt cggcgatccg atggccgaac gccagatgct ggcgctggca
    28321 cacgggtcgg atcaagggct ggccgatcaa tgggcgctgg tcgcgccggc gctggcgcgg
    28381 gccgatcgtc tgtcgggctg cagccgcgag cagtgcgcgg cgctgcgcgg ccagcttgcc
    28441 gccgtcgggc gtctggggcg gctcaccgcc accgaaccgc tcgtgcacga gattcccccc
    28501 tggatcgagc ccgtcggcga ttacgtgggg ggcgcgcggc cggcggagcg cctgttgcgc
    28561 ggcgtcgtca tccccgaaga ttcgttcctc gtcgtgcaga ccggcggctt caacacctgg
    28621 ctggatgtca agtcgttgtt ctccaccctg gaacgggtca tggcggacga gccgcggctg
    28681 cattttgccg ccaccggcgg gccgatcgcc ggccaccatg agtcggggtt tgagtggttc
    28741 caggcggccg tcggggccag cccccatgcc gaccggtttc atctgctggg gtggcggccg
    28801 ctggccgacg tgccgcggct gctggccgag gccgacctgg cgatcaatct cgacctgccc
    28861 tgcattgaag gccgccacgg cacgcgtaac cgcctgctgg actggctgct ggcggaactg
    28921 ccggtcatct ccacgcccgg gtgtgagctg gctgaggaac tgggccaggc gggctacctg
    28981 ctgctgacgc cccatggggc ggcggaccgg gcggcggcgg cgatccgcaa ggtgatggcc
    29041 ggcgcggagg agtttcgcca ggccgcgacg cgcggcgcgc actacctgcg cgaggtccac
    29101 gccccggagc gctgcctggc gccgctgctg gcgtgggcgg ccgagcccaa accggcatcc
    29161 gatttgcatc tgtggcggcg ggggaccgcc gcgccctcgg cgttgcacgc cgccgccgtc
    29221 gatgccgccg cgctggcttg ccggctcgcg gccgaacagg cgcagcggct ccggctcgaa
    29281 caagagctgg ccgaactgaa gggttcgcgg ctgatccagt gggcgctgcg cctgcgccgc
    29341 ggcggcccgt cccgcccccg ggcgtggtag cccccagggg cgccgtctat tgtgaaattg
    29401 ccggttgcgg tatcgcggaa tgccgttgcc ggatcggcgg gacccggccc atccggatca
    29461 atcaaacact ggcagacaaa ggaaaagccc cttgcctcgc ccgatttgcg gcatctacct
    29521 cacgaacgaa caggtcaatg cgctgtgcct gcgcccgacg cgcaagggtc tggccttgac
    29581 gggcgcggat gccgctcccc tgcccgaggg ggatgacgac gcgctgggat ccgcgctcaa
    29641 gccgctggtc aagtcgctgg ggctcgccgg cgccgaggcc catatctgcc tccccaagca
    29701 gcaggcgatc ctgcgcgacg tgacgctccc ctcgacgcag cccgacgaac tgtcgcagat
    29761 ggtgcgctac gaggcggagc gccacatccc gttccacgcc gaccgccatt gcgtcggcta
    29821 tcacgtgatg aaatcgcgcg gcatcgaggg ctccgaggtg ctgatcgccg ccgtcgacgg
    29881 gccggtggtc gaacgggccc tgtccggcgt ggccgaggcc ggcctgcgtg tgcgcggcgt
    29941 cacgctctcc tcggtcgcgc tgatgaacag cctgctccac gtccggcgcg agtggatgga
    30001 gggcaagacg atcgcgctgc tgtgcctggg actcgacacg gtcgacctgg ccctcgtgca
    30061 ccagaacaac ctgatctacg cccgctcggt cgtgatggat ctgcgcagcg tgctgaaatc
    30121 atggctgggg ctggacccgg cgacgcccga ccgcgaggtt gagattgaga aactggccac
    30181 cgcctgccgg atgatcgact gcctggacct ggaagcgcag tacagcgaga gcaagtcggt
    30241 cgcccgcgag gaccatgtcg cgctggtccg gggctggctg aaccgcctcg tgcaggaact
    30301 gcagcggacc tacgatttcg cccgccgcga gatgaaatgc ccgcagatca ccgccgtcgt
    30361 gctgaccggc gagggcgcgc tattgcgcaa tatcgagcgc tacctctacg tcaacctcaa
    30421 cgtcgagatc cagacgctca atccggtggg ggaactgacg cgcagcctga agaagaagat
    30481 gccgttcgac gggctggagt tcgtgatccc gtgcggggcc gccctgaccg acgagatgac
    30541 cggcggctac cggatcgacc tgaccccgcg ggagttctac cggcggatgg cacgccgcca
    30601 gacgctgcgc cagctcagcc tgaccggcgt tctggcggcg atcacgctcg tcctcggcgt
    30661 gctcagtctg ttccatctgc gcgagattca ggcccgcgag ctgaaggact accaggacgc
    30721 caaccgccaa ttgcgccccg cggtcaacga gctggaggaa aaacggacga agctggggat
    30781 catccagggg ttcctcaacc cgcgcaattc cgcgctcaac gtgctgctga cgattaaccg
    30841 cacgccgggg atcgcctcgc gcgtccggct gacgcgggtg gtatttgaaa agggcgagac
    30901 cgtcacgatc gaagggacgg ccaagacggt ccccgacatc acgacgtttg ccggcgatct
    30961 gcgccagtcc ggccagttcg cgtcggtgac gatcccgcgg caggaaccgc gcgagttgcg
    31021 caacctgggc gagaccgtct ggaatttctc gattgaagcg ccgttgctgt ccgatgccgg
    31081 aggcgaccga tgaatcgccg cgaacggatc ctgctcatcg tcacagtgct gatcgtcgcg
    31141 ccgctgctga tctatcattt cttcctggcc gattggctgg aggggatggc gggccaggcc
    31201 gggggggatc tgtcggtcga acgcgcccgg ttcgcctcga acatcgccac gctggccgac
    31261 cggcggcaga tcgagaccgc ctacagccgg attgacttca gccagtccca gccgctgccc
    31321 ggtcagaatg cgaccgaaca cttcctcaat gaactctacg gcctgctgac cgaggaattt
    31381 cagatcatga atccccggat tgaaaaggcg cggccgcagg cgattccgga tgtgccggag
    31441 tactatttcg tggagctgga agtcaccgtc gaggggagcc cggaggagat gctgcgcctg
    31501 ctggtcaaca tggaaaaccg gggggtgctg atcaagagcc tgacgatcga cacgcggagc
    31561 ggcggaggtt tcgggcgcga cgacgagctg gtcaacctga cggttgaagt cgcccggctg
    31621 gtcaagcata ccgaggagtc gatgaagatg atgcggggtt tcgggtcctt ttgatcgggg
    31681 ctggcatcga cgggatgatc gccatggact cgatggacga gggacaagag ggacgtccat
    31741 tgactggatg atggccgggc tgggcatgcc ggcttcccgc acagccttga cgcttccggg
    31801 ccgggcgcgc taatctcaac ttgtatgtca tctggaccgg agcgctcgtt cggcggcccc
    31861 cctccgccct ctccggccac aaccccagga gctgtcaaca tggcgagcga taaggttctg
    31921 cacctgaccg atgaatcctt cgacacgacc gtaaacaacc ccgatgtccc cgtgctggtt
    31981 gatttctggg ccgactggtg cggaccgtgc cgccggatcg cccccatgat cgacgaactg
    32041 gccgacgaat acgccggccg gctgaccgtc gccaaggtca atatcgatga gcatcagggc
    32101 gcggccacgc agtgcggcgt ccagtccatc cccacgctga tcctgttcaa gggggggcag
    32161 atcgccgagc ggatcgtcgg cgccgtgccc aaggcgatct tgaccgaggc aatcgacaag
    32221 atcctctgac gcccggcgga ccctcaccag tctggagcgg aggggtgcgc gacgcccctc
    32281 ttctcatctt caacgccgtg gcctgcgcgc ttcatgtcac tgccgcccct gctcgaacac
    32341 gacgccgatc ccgatcccat cgcccagttc agccgctggt atgccgaagt ggaggccgcc
    32401 ggccaggccg aaccgaccgt gatgacgctg gccacggcca cgcccgaggg cgtgccgtcg
    32461 gcccggatgg tgctgctgaa gcacgccggc cccgatggtt ttgacttcta taccaattat
    32521 gacagtcgca aagcggcgga gctggcggcc aacccgcgcg tggcgcttgt cttctggtgg
    32581 gaacgcctgc ggcgccaggt ccgggttgaa gggacggtcg ttcggctgcc gtcggcggca
    32641 tcggccgctt acttcgcgac ccggccgcgc ggcagccgga tcggcgcgtg ggcttcgccg
    32701 cagagccgcg tcctggccgg ccgggccgag ttggagcggc gcgtcgccga gatcgagcaa
    32761 cgcttcgccg gacaggatgt gccccttccc ccgttctggg gcggctaccg ggtccatcca
    32821 tggatcatcg aattctggca ggagcgcggc gaccgcctgc atgaccgcct gcgctaccgc
    32881 cgcgaggggg acggctgggt gatggaacgg ctggcgccgt agcgccacgg ggggggaggg
    32941 acgcgacgtc cccgccgtgc tatcccgccg cctgctccgc cgcgccgtcg agtttcagaa
    33001 tccgcgccgc atcctcgggc gggaatgggt tgatgtagaa ccctgttccc cactcgaagc
    33061 cggcgggctg gctgagtttc ggaatgattt ccatgtgcca gtggtagtag ccgttctcgg
    33121 tctcgcgcag cggcgtcgtg tgcaggatcc agttgaacgc gggatcgtcg aggacattct
    33181 tcagccgcga catcgtctcc ttgagcacct tggcgaattc cgtcacttcg ttcttctgaa
    33241 tgtcgtggaa gaacgaggtg tgctccttcg gcatgatgct gcattcgaac ggaaaccgcg
    33301 aggcgtaggg caggaaggcg atgaacagtt cgttttcggc caccaggcgc atctgatcct
    33361 ttttttcctg gtcgatcatg tcgcagaaga cgcagcgctc cttgtagtcg aaatagcgct
    33421 gcgcgccgtc gatttcctcc tgcacgcgct tggggacgat cggcgtggcg atgatctgcg
    33481 aatggggatg atcgatcgag gcgcccgcct cgcgcaggtg attcttaaag atcatcacgt
    33541 attccagccg cgggtccttg cgcatctcga ccgtccggtc gcggtaggcc cagatgatct
    33601 cttcgaccat cttgacttcc atgtcgccga catgttcatc gtggcggggg gtttcgatca
    33661 cgacctcgtg catgcccatg ccgttgatcc ggtcgtacat gccgtcgccc gagcgcttga
    33721 ccggctcgcc gctgaccagc gccgggaact tgttgggcac cacgcgcagc caccagccgg
    33781 cctcgttgtg gtcggtgccc ggctggcggt aggccagcac ttcgggcggc gtcatgtgtt
    33841 cccgccgcgg acagaaggga cattcttcat tcgaatgggt cggttggcgt ttgacgggga
    33901 aggcggcggg gcgcttggcg cgctcggtgg cgatgatcac ccaccggccc gtgacagggt
    33961 ctttgcggaa ttcaggcatg gtccgttcgg ctttccggaa aacacaagtt gcacgggaag
    34021 cgggcgccgg ttggggctga cagcgcatcc cgcgaggccg ggagggtgcc ctgaaacaat
    34081 tattaaagag gggccggcca ggggtgtcaa cacggatgaa cactcacgac cgggggcgcg
    34141 cggttggcct cgggagcttt cggggaagga acacggaagg gaccgaagaa acggaaagcc
    34201 ggagctgaat tcctgggagg gccgccgggg ccatcggcgc cggcagaaat aaaccggcca
    34261 cgccacggtg gccggtctgc atccggcaga agagggagga tcgtcagctg tcgggccgtc
    34321 cgcgccggcc gcccgttttt agtgcattcc tccggcgggg gaacggctcc atcgacagcc
    34381 aacgattgga ccgcgcggcg gcgtgcacgg tttggccgga acggccgccc cgctcctaca
    34441 gcggcgtcac gaacggctca ggcccccacc ggcaacgccg ccgtgatgcg ttcaacgact
    34501 ttctccggcg tgaagccgaa gtgctcggcc agcgccttct ccggcgccga catgccgaac
    34561 tgatcgatgc cgatcgccag gccgtccgcg ccgatatact tgtgccagcc gagcgtcgcg
    34621 gccgcctcca ggctgacccg caggccgacc tcgcccccca gcaccgcttc acgataggcc
    34681 gcgtcctgcg cctcgaaccg ttcccagcac ggcatcgaga caacgcgcac gctgcgcccc
    34741 tgttgctcca gcaacgcggc ggcccgcatc gccagcccga cctccgaccc ggtcgccgcg
    34801 atcagcacgt cgcagcgccc gcccggctcg cccttgagca catagccgcc gcgggcaacg
    34861 ccctcgcgcg cccggctgcg cgtcgcctcg ccctggctct cgatcccctg gcggctcagg
    34921 atgaacgcca ccggcccgtc gcacttccag gcctcgatcc acattgcctt gagctcgttc
    34981 tcgtcgcacg gccggaagac cgtcagattc ggcatcgcgc gcagcgcggc cagctgctcg
    35041 atcggctggt gcgtcggccc gtcctccccc aggaagatcg aatcgtgcgt gaactggaag
    35101 aagacccggg ccttcatcag cgccgccacg cggatggcat tgcgcatgta atcggagaac
    35161 gtgaagaacg tcccgcagag cggttggacc atgccgtgga gccacatgcc gtagttggcc
    35221 gccgccatgg cgaattcgcg cacgccgaac ttgatgttgc gctgcgtcca gtccgcgcgc
    35281 gtcacgaact ggctggactt gatgttggtc aggtccgaac aggacagatc ggccgacccg
    35341 gtcacgaaaa acggcgccag ctcggcgact ttggcgatca actgctggcc ctgggcgcgc
    35401 gtcgccttgt tcggcgcgac gtcgaggttc cagatcgcgt ccgcatagtc ggccggcagt
    35461 tcgccgttcg cgcacgtctc ccacagcttc gctttttcgg gatcctgcat cagcgcggcg
    35521 agcttctcgt tccaggcttt ttcataagcg gcaaactgcg cctggcggct cttgaagaac
    35581 tcgcggacct cggtcgggat gtggaacgcc tcgtcgggcc agcccagggc cttcttggtg
    35641 gcggccagtt cctcggcgcc cagtttcgcg ccgtggctgg cgctcgtccc ctgcttgttg
    35701 ggcgccccct tgccgatgat cgtcttggcg atgatcagcg tcggccgggt gttttcgtcc
    35761 caggcggtgg cgaacgcctt cttgatctca tcgaagtcgt ggccgttgat ccgcagcacg
    35821 ttgaagccgt aggcctcgta gcggcgggcg gtgtcctcgc tcatcgcttc ggccagcggg
    35881 ccgtccaggc agatgtcgtt ggagtcgtag atgatgatga gattctcgag gccgaggtgg
    35941 ccggcgaggc tcccggcctc gctggtgatc ccttccatca tgcagccatc gccggccagg
    36001 gcgacgattt tggcgctgaa cagctcggcg ccgaagcgcg cgcgcagcca cttctgggcg
    36061 atcgccatgc cggtcgccgc cgccacgccc tggccgagcg gtccggtggt cgtctccacc
    36121 cccggcgcat gcgcctcgtg ctcgggatgg cccggcgtgc gcgagccgag ctggcggaaa
    36181 ttcttcagct cgtcgaggga caacgcgtag cccgacaggt ggagcagcga atagaggaac
    36241 atcgagccat ggccggccga caggacgaat cggtcgcgcc ccatccaccc cgggttcctg
    36301 ggattgtggc gcagggcata ggcataaagg taagcgccga gctccgcgca gcccatcggc
    36361 agcccgggat gccccgaggc cgccttctca acagcctcgg ccgaaaggac gcggaccgtg
    36421 tcggccacct tctggaggat ctgtttgtcc tggctggtca agggcgcaag acgcgtcgaa
    36481 gcggatgggg tggtcatgat gctcaactgg ctcctttgga agtcggcggg cacaacccgc
    36541 gttgggcggg aatcgcgtgt cccgcccggc gtcggtgggg tccggtcgtt gccgacgcgg
    36601 acccaaatat cccaagctat cccgtgaagg aaggagagtc aacacggcaa gccgaaccgg
    36661 gcgtcaggcc gcgggggctt tagccggata cttgcccggc tccttgacca cccggtcgcc
    36721 gacctgcagg gaatgaaaga tcgcctcgcc gacggcgagc cggccctcct cgccgccgtc
    36781 gctgcggtaa tagatatagt agtgcgggag atcgcggccg tgtttcgcgc tgcggcgatc
    36841 cttgctggtg tatttccgcg tgatctgccc cgtccacgcc cgctgcatcg ccttgcggcg
    36901 gttgaccagc accaggatga tcgccccgat gacgaacacg aacatcccga cgacaagaaa
    36961 tggaagcagc atgaagatgg tggcgttcat cgggggagtg cacctcggtc ggaggtatca
    37021 tagcagggcg gtagcccgtc gcgaagcgct caaattcgcg atgcnnnnnn nnnnnnnnnn
    37081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37141 nnnnnnnnnn nnnnnnnnnn nnnncggagg tatcatagca gggcggtagc ccgtcgcgaa
    37201 gcgctcaaat tcgcgatgcc cccaaatggg gaccgggggg ccgagcgaac cgctcagggg
    37261 cgccgattgc tctctggttg gttttggcgg aaatttcggt tgacacgcca gcgattaatg
    37321 cgatatatag atcgcaatat gtggatgtcg cctggctaac cgctccgttc tttccctccc
    37381 cgacatccac ggcggactgg attccgccct ccgccgacca cccgcccgag cgctcgttgg
    37441 cccgacggtt gaaccaccca tcgagaggcg cctgatcatg agtatcatct cgcgcaccgc
    37501 gcaatacgcc ctccgctcga tgaccttgct ggccattcaa cacgatcgcg tcacggtcaa
    37561 ggatctggcc caggaggtcg gcgcgcccca gccctacctg gcgcgcatta tcatgttgct
    37621 ggccgacaag gggctggtcg acgcgcgccg cgggccgggc ggcggcctcc ggctggctcg
    37681 acccgccgag gagattcgcc tgatcgacat cgtctcgctg ttcgacggct cgaagctgtt
    37741 cgaagaatgc gccctgggtc tgcccggatg ccgggagggc gaacacccgt gtccgatcca
    37801 tgaccagtgg ggctgcatcc gcgaacagat ccgcacctgg tggcatgata ccacgctggc
    37861 ccagttcaag ccggagcaga cgctggagtg catgcgcgag cgcgtcaagc accttccggt
    37921 ggaaaccccc tattcgcggg gcaatggccg ctggcgctga cggcgactgg atttcttcca
    37981 catcagggga cgggggcaag ggcccgccgg cgaccgtgcc ggtgggccct tttgcatttt
    38041 gaatacgagg aatttcgaga gccgagagcc gattgttcga taagggacgg gcggttgggc
    38101 ccccaaaaaa aatccttcat ctgtgatata atgaaaagtg ctgccattct ggtagcatcg
    38161 tgaccctgcc taccccgcgc atctgatcgg accgagcgcc gatggccgac cgactcgaga
    38221 tcaccccggc gcagttcgcc cagcaggtca aggaccggct ggcgctcgac cagttgatcg
    38281 ccgaatacgt cccctcgctg aggcggtcgg gcaacagcct gaaaggcctt tgcccgttcc
    38341 acaaggagaa gacgccgtca ttcaacgtcc atgtcgagct ggggttctac cactgtttcg
    38401 gctgcggggc gcacggcgac gcgatcaagt tcgtccagga aatcgagaag atcgacttca
    38461 tgctggcgct cgagcagctc gcgcggcggg cggggctgca gatgccgcgg ttcggccggg
    38521 gggaggggcc ggcgcggccc gagcgggagg aacagctggc gcggctgaag gcagcctgcg
    38581 cctgggccga ggcgttcttc atcgagcaac tggagcagca tccgcgcggc gccgtcgcgc
    38641 gcgaatacct gcgcgggcgg gggctgacgg acgaggagat cgcgcgctac cggctgggct
    38701 acgcgcccga cggactggac gtcctgctgc gcgaagggga acggcgcggc tggagcggcg
    38761 agacgctggc cgaggcgggg ctggcgagcc ggcgcgagca gggcgggttc ctcgaccggt
    38821 tccgcgaccg cgtgatcttc ccgatcgccg accgctacgg ggcggtggtg gcgttcgccg
    38881 gccggctgct cgacagcgag cgggaagcgc cgaagtatat caactcggcc gaaacgccgc
    38941 tgttcagcaa gagccggttg ctgtacggga tggcgctggc gcgcgacgcg atccgcgaat
    39001 cgcgccaggt gattctgctc gaaggctaca tggactggat cgcgctgcac cggcgcgggc
    39061 tggccaacgc gctggcggga atgggaacgg cgctgacgga ggatcaggcg cggctgatcc
    39121 gccggatcgc cgaccgggcc acactgatct acgacggcga cgaaccgggg cagaaggcgg
    39181 ccttccgggg gacggagctg ctgctgcgcc agggcttgga agtgcggatc gtgacgctgc
    39241 cggccgagca tgatcccgat accttcctgg aggccgaggg gacgcaggcg ctgcgcgaac
    39301 gaatcgcgga agcgccggcg gcggtcagtt atttcacggc tcggatcggc gagcgggcca
    39361 atttgtcgcg cccggagggg aaagccgacg cggtccagca gatcgccccg ctgctgctgg
    39421 cgctgggcga cccggcgatt cgcgaagggt atctggagca ggcggcggcg cgcttcgggc
    39481 tgcgctacga gacgctggaa gcggcgttgc ggcggcgggc gcccaggcgc gtcgggccgg
    39541 ccgccgaggc cggccaggtg gacgcgggtg ccgcgccggg ggaaccgggc ggggactggc
    39601 cggtcacgga acagatattg ctcttttcgc tgctgcattt tgaggcacgg tgggagctga
    39661 tccagcagat cgatgccgaa tggttcaccc acgaagagct gaggggcttg tatcaaagga
    39721 tttatgaagc gcatcgcgac gtgcgcgagg gggcggatcc gccgacggac tggtttgcgc
    39781 tgtgccatac gcccgccgag gaagcactgc tgagtcgcat tttgttgctg cctcagacac
    39841 atttccgcgg ggatgtggct gatttcagcc acacgctggc cgacgccatc cagcttcagg
    39901 tcttgaaatt gaaacgcaac tggacccaac gcaagcgacg ggagctggcg cgcgatctgc
    39961 agatgattct ggacgaacac cccctggggc ggggccaact ggcggcgatc gacgagttga
    40021 cgcgccgcca gttgcaggag acggccgaat ttttccggcg caacgaataa acgcgacgaa
    40081 cctttggttc ggctgatgcg tctaatggag ggaggagcga cggcggcggc agaccgccgg
    40141 ctcgccttga ttccttcgcg gggtcgaaac accggcagca ggccacgggc gggatcagcc
    40201 agggtgctgg gcgccgatcg cctcgccggg gcgttttcga cttgacccgg gggtttgcgt
    40261 caaacgcccc cgaagcgcag ggggagaatc aaggggtggg caggccgatg actgagaatc
    40321 cgcttggcag cagccatcgc ggaggagtaa attatggtgt ttaccgtgcc ttcgacggtg
    40381 cggacgatcc ggggcgaggc gccacaccga cccgaatccg ccgccgaaga gcttgaggag
    40441 gttccgcaac acgcttatgg cggttcagga aaaaaatccc tttgaggcga tcatcgaact
    40501 cggcaaggaa aaggggcatc tgacgttcga cgagattaac gaacggctgc ccgaggacat
    40561 caccacgccc gagctcgagg tcctgtttga ggaactcgat cagcttgaaa tccccgttct
    40621 ggaggaagag gccgaggaag agagcgaggc gccggcgcgg gaggaaacct tcgagcgcga
    40681 cgagtatcag gacgaacagg ccgcggcgga cctggaagcg gccaagatcg atgacccggt
    40741 gcggctctat ctgatggaaa tgggcaaggt gcccctgctc acccgcgatg aggaggtgag
    40801 cctcgccaag cagatcgagc acggccggca catgatcacc cgcgcgatcg cgcgcgcctc
    40861 ggtgaccgcc gacgagatcc gcagcatcca cgagcggctg gagaacaatc tttacaacct
    40921 gaacgacctg ctgcgggcga acgtggacga ggccaacgcc gacgagcgcg ccaaggtggt
    40981 gcgccgcacg ctggaccagc ttgacggcgt cctctcgatc tacaaccaga tcgatcagga
    41041 tctggaggcg atcgaggacg cggcgctgag cggcgaggat ccggcggcgc tggaaaaggc
    41101 ggtcgaggag cggcgcgagg cgatctacca gaagctgctg gagatcgatc tgaattacgc
    41161 ggtgatcgag cagattgcgg ccaagatccg cacggtcttc acgcggatcg acgaggcgaa
    41221 ccaggaaatc cagtcggtca tcgtcaagac gatgatcagc gaggaagagc tgcgccggat
    41281 cgtcaagcgg accaagcgca actcgcccga ggccaaggcg ctgccgcgca agtatggctt
    41341 cacgctggac gacgcaatca agatggacaa gctggtgcgc aacgcccaga aaaccatcaa
    41401 acgcatggag aaggaagtcg gcaacagcta cgaggaactg caggagatca tccggcagat
    41461 cgacgtgggg gagaaggaag cgcacgaggc gaagatgaaa gtggtcgagg cgaacctgcg
    41521 cctcgtcgtt tcgatcgcga agaaatacac caaccgcggc ttgcagttcc tggatttgat
    41581 tcaggaaggc aacatcggcc tgatgcgggc ggtggacaag ttcgagtatc agcgcggcta
    41641 taagttctcg acgtatgcca cctggtggat ccgccaggcg gtgacgcggg cgatcgccga
    41701 ccaggcgcgc acgatccgca tcccggtgca catgatcgaa acgatcaaca agctgagccg
    41761 gatcagccgc tacctggtgc aggaactggg ccgcgaaccg acgccggagg agatcgcggc
    41821 gaagatggac atgccggtgg agaagatccg ccgcgtgttc aagatcgcgc agcagccgat
    41881 cagtctcgaa accccgatcg gcgaggatgg cgacagccat ttcggcgatt tcatcgagga
    41941 ccaggacgcc accagccccg tgacggcgac ggcctaccgg ctgatgcagg agaagatcga
    42001 agaagtgctg gggacgctga ccgagcgcga ggaaaaggtc ctgcggctgc ggttcgggat
    42061 cggcaacaat gacttcccgc gcacgctgga ggaagtcggg acgatcttca acgtgacgcg
    42121 cgagcgcgtg cgccagatcg agacgaaagc cctgaacaag ctgcgccacc ccagccggcg
    42181 caagcggctg atcgaattca tggaatagcc cggctcaaga agcgagaagc gtgaaggccc
    42241 ccggctccgg ttggatcggg ggcctttgcc gtttgcgtgt cgcggggttc ttagtcggtt
    42301 tccggcagga cgggttcggc cggatccacg cgttccttcc ccggcgccga cggcttgcta
    42361 tccccgccgg ccgcatcagc ctcgtctttg cccgccgggg gctcgatgaa caggatctgc
    42421 tcgcccggct tgaccatgat gaatcgctcg cgaatcgcct tttccgccgg gaagcccccc
    42481 gcctgcaacg ccgcgcgttc gccgatcatc tcgtcgcgca gccgctcctc ctccgccacc
    42541 aggctgcggt agtgttcgag ccgttcgcgt tcggagagat agcgctgcag ggtgtcgtaa
    42601 ttgacgaaca gccatgccgc ggccgtcagg gcgaggcagg ccaccggcca gagcaaccgg
    42661 gcgggtcgaa accagaagaa gagccaacgc agcatctggc gtggaaccgg tcgatcggag
    42721 tcggcgggtc ggccagtggc cataacccgg tgcgtcggca cgcacggcaa cgggaggaaa
    42781 accccaatcc acgtctgcgg ccattatgca tactccggat tggattgtct tgtccggaga
    42841 tatgatgccg ttgcgcgtcc tcgtcctcgg tcgttctgca tgatctcgcg gaagagctgc
    42901 aaggaacgta aaggacataa ggagcataag aaagacagat cggccggtaa gcggaggacc
    42961 ccttgcgtgc ctatgatgat gttcgtgatc cggcgcttcc ctgagcggct ccgcggcaaa
    43021 tcgattgcgt ctgcgccggc ggcccctaga atgtgacgcg ccgccaggtc cgcgcccact
    43081 ccacccagcc ccgaggcgcc cgccttgaac tccgccccgc cgcattcgtc gctccgcgag
    43141 gccattccca accgccggca actggcgctc gatctgacgc tgctggcggc gatgctcgcc
    43201 gtcggtctct atctgcgcct cgccaacctg acgctggtcc agcattttca gggggatcag
    43261 ggccgggatt acatcgtggt ctgggactgg attcgcgcgg gccgctggcc gctgctgggg
    43321 ccgtatcgcg gcgttgccgg cgacttcgcc atcggcccgg gctactttta cctgatcgcg
    43381 ccgctgatgg tcctgaccgg cttccacccg gtgtccggac ccgccacgat tgcctgcatg
    43441 aacgcgctga tggtcgtgct ggcctggcgc tgggtgctgc tgtccacggg cagccgtctg
    43501 gccgccctgt ttgttccgtt caccattctc ttttcggcgg tctggacggt caccgaccgt
    43561 tacaactgga accccaacac gctgccgctg gcgacgatgc ttatggccgc gctgatcttc
    43621 gggctgcgcc gccggcccgt gccgtgcctg accggttacg tcatgctgtg cgccctgctg
    43681 ccgcacctgc atacgacgac gttgctgatg atcgccgttt ccctgccgat cgtgctgtgg
    43741 gtgctgctgc gcgaacggcc cgactgggcg cgcgaccgcg gccaccgacg ctgggcgtgg
    43801 atcgcactcg ccgccgtctg gctggcgctg ctctacacgc cgccgctgat ctacgagatc
    43861 ggcccgcgca ccaacctgaa gggttacatc gcgcgctcga tcccgccctc gccgccgccg
    43921 gaagggccgc cgctggcccg cgcgcatgat gcgctcgacc ggctgggccg ctggacgatg
    43981 caccacaact ggcccaatcg ctggccggtc cattaccccc gcaacgtgct gatgctggcg
    44041 tcgatcctgg ccggcgcggc cgcgatgctg gcgtggtcgg tggggcatcg ccgcgcccgc
    44101 cgcgccgacg gcgaatgggc cgtgctgtac ctcctgctgc aattgggggg catgctctac
    44161 gtcgcctttc agaagggcga caacttcgag cagtattacg ccaatcagat cctggccatt
    44221 ccggtcctgc tgctcggttg gctgctgggg atgctgttga gcggcatggc ccgccaactc
    44281 gggccggccg gccggcggcg ggccgcggtc gtctgggcgt cgcggctgct ggcgttcgcg
    44341 atcctggtcg cctgcggtcg ctggacggtg acgcaactgc ccgacgcgat cggcgtccac
    44401 cggggcaagg tgtggtacga gtttcaattc gtcaggacgc aggagatcgc gcgggcgatc
    44461 atcaacgccg cccggggcag gccctacagt tttcagtatt tcgggccggg cggcgtacgg
    44521 gatcatttcg aatacctgct gcgctacatg ggcaacggcc cgcgcaacga tccgggcgac
    44581 ggccgccgca tccgcgctcg cgacctgggc agcgccctgt tcatcctgac gcagaacgaa
    44641 gccaacgcgt ggcgcccctg gttcgagagc gcggcgaccc ccgccacgcc ctccccgctg
    44701 cgaattcatg acgcgaacat ttatgagatc ccgcgcagcg cgatgccgct cgagctgcgc
    44761 tgggtggagg tcgtcgagcg cgacggacgg atcatcatcc gctggcggga gcgggattga
    44821 gtggtcgccg gaccgggccc ggtgctgatc atcatggccc cgagggagtc agcttaagtc
    44881 caccgctgcg gggtcccggg agagcgatgg ggctcatggc gcgggcggtg cggccgataa
    44941 gaccagtggg gtggtgcggc cgcgtcgcgg cgcccgccca cttgatccgc gcgccgacgc
    45001 cgtcggccgc cgcggctgga gcggcttctg gtgacctcgg cccacaccac gacgggacgc
    45061 ccgctgcgcg agcgcgtgat cgcactgatc gaccgcggcg tcagccttcc gccgctctcg
    45121 ccggcggccg cccagctcat ccgcatcctt ccccagccga tcgatcgcat cgacatgcgc
    45181 aagctggtcg agctggtgca ggaggacccg acgctgtcgg cgcatgtcct gcggctggcc
    45241 aattcgccgt tctgggggat ccgtcagagc gtctcgacgg tcggcctggc ggtctcgctg
    45301 atcggcctgc aagaggtcgt cggcacgctc aattaccatc tgctgcgcca gctgacgccc
    45361 aacgtcaagc gctcggccca cttttcgccc gacgccttct ggctgcattc ctggcgcggg
    45421 gcgaccgctg cccggctcct cggccgaccc gatctgttcg tcacctgcct gcccggccaa
    45481 ctctacatgg ccggcctgct gcacgatatc ggcaagctga tcctggtgct gcacttcggc
    45541 gaggaatggg aagcgtgcgc cgacgaggcc gcgaccagcg ggcgaccgct acacgaggtc
    45601 gaacaggagc gcatgggcgt ggatcatgcc gaggtcggcg cgcgccagct cgaagtctgg
    45661 ggcctgcccg acctgattcg cgtcgccgtc gactggcatc atcgtcccgg cgagctgccc
    45721 gaaggccccg cgcgcgagat ggccagcctg attcagttcg ccaatgccat cgcgcacgcc
    45781 tgcacggcgc acgaagtggg cacggtcccg gtgtgcccga tctcggaaac ctggctcggg
    45841 atgcacaagg atcatccgct gtcggatccg gtcaagcgcg aagggattgt ccacgaggtg
    45901 atggcggcgc tgctggcacg cgaacgccac ctggatgacg gcgaggcaac gaacgcggcg
    45961 gaggaggagc ctgcccggcc ggcgcccatt gccgcgccgc gcccggttga gcttgccccg
    46021 gcgcgccaat gg
//
LOCUS       DAOPCO010000006        44751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_972_length_44751_cov_5.054188, whole genome shotgun sequence.
ACCESSION   DAOPCO010000006 DAOPCO010000000
VERSION     DAOPCO010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 44751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44751
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_972_length_44751_cov_5.054188"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PLS90_01085"
     CDS             complement(<1..1571)
                     /locus_tag="PLS90_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPK01025.1"
                     /translation="MPTAMEKRLGELLIEDQIITEDDLKIAMHKQKETGLSLGRILIN
                     MGRASEWEVAATLGKQLNVPFVTLSHYEIDPEVLESIPRDLVFRYKIIPVDKTGDTLT
                     IALADPSNIYLLDELKLLTHCQIIPVISFESDIQEAIGKYYKKDQEENFHEALKDITD
                     QELEVLEEERQAVQAEEDDDLSLEADDAPVVQLVNLMIAEALKMGASDIHVEPYERSL
                     RLRYRIDGVLQEMPPPPKKFQNAIISRIKILSDLDIAEKRLPQDGRFKVRQQNKSVDF
                     RVSCCPVAHGEKVVIRVLDQSNLQVRLTDLGFEAPVLRKFESEIRKPWGMVLVTGPTG
                     SGKSTTLYSALSTINDPSKNISTIEDPIEYQLQGINQVAARPDIGLTFAAGLRSFLRQ
                     DPDIIMVGEIRDLETAEIAVKAALTGHLVLSTIHTNDAPSTVNRLTNMGVEPFLVTAS
                     LNMIVAQRLVRRICNECKEEYTPSPELLDAIRIPAKANQPFYQGAGCQVCNGTGYKWM
                     LGGRSTISAGCQVCNG"
     gene            complement(2034..3299)
                     /locus_tag="PLS90_01090"
     CDS             complement(2034..3299)
                     /locus_tag="PLS90_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01026.1"
                     /translation="MALPPSGTLVICRPFAPCATPALPAGLHFALTTAAQGRNERFSL
                     REAAESFERAVVEQSLGEELAEAGVRLQLAAIPDFVRRVEPRGAHPRLLILVSNDTHA
                     RFMIPAAEGLGDVLFLIPPRGLRDEGAAETLQSEGFAFVELTPGVEELVRAFGPRLML
                     CGNDWSEEFQHARRLCRRLGIPSVALQEGPQDWEAEWGGRNPRKYLNADIVLAQGAVT
                     LRHIRPRCFAVTGNPKVDRFEPIALPERPRILINCNFTYGQYEEARALWMHDVLGACR
                     ELGLDYFISQHPRDPSSWEGEPVVRSAAERLAAQIAEASLVVTRFSNLPYEALAAGRP
                     VIYYNPHGEPMKTFREDRCASLGYATRPGQLRRLLRRHQLRPEMDREGTLAFLRRHCG
                     SMRGRAQENLVSLLRAIPQGAAEAGRIRL"
     gene            complement(3303..3932)
                     /locus_tag="PLS90_01095"
     CDS             complement(3303..3932)
                     /locus_tag="PLS90_01095"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPK01027.1"
                     /translation="MTRRIHDISRTIASGGFVYPGDPPIALEPLSTIGVDGARCNMRM
                     LHMNSHTLTHLDAPRHFVAAGATLDQLPVERFIGPALVVAAPRGVIEPEDLPGDELRG
                     RNILFQTAAADQYETGRLDEPDGGLSGAAAEELVRRGVNLVGIDTPSIDRFGADDFPA
                     HTALLGAGVLILEGIDLRAVGAGWYTLIALPLKLIDADGSPCRAVLVSD"
     gene            4543..7347
                     /locus_tag="PLS90_01100"
     CDS             4543..7347
                     /locus_tag="PLS90_01100"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPK01028.1"
                     /translation="MCGFVFLSAPLPALGPNVLVNGGFENPYPDDVYAPDERPEGWSL
                     EDGNDDQFLRGREYVWIDGVLMPQETVKEGRRCIKIQGTWHGDETGPRIYHDKLAVAR
                     GLDNDAYYLLQFWYRTSGIERADFFVRVNHYTNYPENPQTRPSSFAESENDLLPAEDW
                     TPVSFLIRPIQEDQAPYAYLPDKYPNFRGTDALRIMFGLNNTSGCVYIDDVMLRKMDQ
                     NGSEAHMIPLNSNQVDPPAVDGLKPVFPPTDFYTIQRDERGVWWIVRPSGDPVWIIGM
                     NNPEDPGSRNPAMSQWIQQHFAPGTYGDDVVRRLKAWNFTATIENDTPGSARNWKAEQ
                     RYMDFSGDSWVSKSWRMREADGGYWGDDGAYLADPYNPDWQARARQEVARDLTASRLD
                     DPEMLGYYTDNCLPCKNLHLGIWSTHCSQVFLNWLATRYPTPQACAQAWSSKYKTFNF
                     SSWNHVYDARADVRIHGFDDSPALIRDLKDFELKVWKDYFRFICTIIREREAAVFGAD
                     PSGMPLKKHLIFTNRFPWNGYPDAGQEGVERMMDAIRELNQERPGFYFDALAANIYPG
                     GMHLPDQKSRQNMLFLERMSQRCGLPVFVSEFAIAGNDVGFPPSVRWRDRTVPTQADR
                     ARAYRNLMFTWSRLNFMLGGMWYQWYDGMYDNGGPSKAGFTGIDPADPKYDGRNSGFV
                     DSQNREYTVFVQQVAEVNAVLRDTSRSNAVSVADFPWDTSGGQAPPPPREPRLIHHSL
                     SGKTYLTWGHTDDRDIWNYVIHYSPRPNCDYTRLAWLGYVTSFEIPADKPGYYRVAIS
                     DSQRNEGPWTEAFHTSQATPLSDLTILSPASGEDVAFGEPIDIRWLPPAPTQQVLVQI
                     QHLVDGQWQVLQQSEWMDNLGEHREWIWQELPDGTQLRAVVTGGNWDGSSFTAASAPF
                     TFDNPKTEQPRTAARHWMYY"
     gene            complement(7341..8345)
                     /locus_tag="PLS90_01105"
     CDS             complement(7341..8345)
                     /locus_tag="PLS90_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK01029.1"
                     /translation="MGAIRWPPRSTLRGGLLPANRPLRLAAVLALSYLAVCALYIRVS
                     TTLAARFAESLVELERLEQLKGLAFIVVSSLVFFGIAYYLLRRISLGELEIERNRQAL
                     LTAGRRALAGALASSIAHDMNNLLMVTDVDSLELARGALQGPETPEQVGARVNGCVRD
                     LMKLSRRLMTIGSDRSQYRPRGLDLAACVRDTLEFARIHKRVRRCALEYAGPEVLGFV
                     SDPVALQQMLLNLLLNAADAMGGEGRILVELKERAPGVVLRVHDSGPGIPPDKRQAIF
                     DAFYTSKESGAGLGLLSVRSCAELHGGRIHVGESARLGGALFEIELPPCQAVGIEQDQ
                     "
     gene            9534..10415
                     /gene="fdhD"
                     /locus_tag="PLS90_01110"
     CDS             9534..10415
                     /gene="fdhD"
                     /locus_tag="PLS90_01110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPK01030.1"
                     /translation="MNTRPRGALRIQASRSESVVDTLAVEAPLQVNINGRPWTTTMRT
                     PGDDPFLVRGMLFSEGLLTDPAAELGWQPFDDPESGHIAAVDLTVAESALNQPFEDRR
                     AQMSVSSCGLCGIRQWRDLFPGDFSPRLNGQIQLAADLIPAAMADLHHGMRAFGESGG
                     CHGAAAYTARGRRLALFEDIGRHNAVDKVIGSLLSRDQLHLAVLLAVSGRISYEIVFK
                     AFRARLPVLAAVSAPSSMAVEMADAFGLTVLGFCRDTRLTVYSHPERIKAQAQPRSRT
                     ARRRAAAPAPAGRRDQP"
     gene            10412..12697
                     /locus_tag="PLS90_01115"
     CDS             10412..12697
                     /locus_tag="PLS90_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HPK01031.1"
                     /translation="MKPIRKAWDPKVWASLRPFGIGLQRPNNYWELIRALLENRDRIP
                     FAWRILTKGVCDGCALGTTGMSDWTLPGTHLCNVRLRLLRLNTAPAFNPRLLRDLKGL
                     AGKRSAELRDLGRLPAPMRRRRGAPGFERISWEEALATIAAKIRGLASPDRIGFYLTS
                     RGMPNESYYGAQKAARAIGTNNIDNAARICHSPSTTALKRSTGVAATTCSYADWLVSD
                     LIVFIGSNVANNQPVATKYIHYARKNGARVAVVNSYREPGMERYWIPSIPESSLFGTR
                     ISDRFFLINQGSDVAFLNGALKHLLANDWIDHDFIREHTHGFDALRESVERMSWEEIE
                     AQSGVPRLEIQAFGNMLAEARRGVLVWSMGVTQHETGVANVQAIINLGLARGWLGREG
                     CGLMPIRGHSGVQGGAEMGCYSTVFPGGRSINAENAAWLAEQWGFPVPDRPGLTAPEM
                     IEAAHRGELDVLFSSGGNFLEVLPEPDYVEEALRRIPLRVHMDIVLTSQMLIDSAEET
                     ILLPAMTRYEIPGGVTETSTERRVIFSPEIDGPRVPQARSEWDVLLELARRVRPELVD
                     RLRVAGTPGLREEIARVIPAYDGIQHLRRQGDQFQYGGEHLCPGGICPTPDGRARFFP
                     VELPDARLPEGVFVVASRRGKQFNSLIHEEKDMITGLGREAVLINPADARKLGLQEGE
                     RVEVRNGKGKLTGRVRLAPIKPGNLQVHWPEGNVLLDHHRRSCEAGVPDYYARVTLHR
                     LNGQAAPRPQPVPAERGVPAP"
     gene            complement(12766..13443)
                     /locus_tag="PLS90_01120"
     CDS             complement(12766..13443)
                     /locus_tag="PLS90_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK01032.1"
                     /translation="MEPKGARGAESGPIRVVIVDDHPLVRHGLGHLIAAEEDMEVVGD
                     ASGSTDALRLVREQKPNCVVIDLSLQEGTGIELIKQIKALDKNIRMLVFSMHDEVLFA
                     ERVLRAGAMGYVNKQVSTEKVVDGIRHIMKGKIFLSEEMSERLLIGMTGREVVGETPV
                     DNLSNRELEIFQLIGQGLSTRKIAEQLHLSSKTVESHRESIKKKLNLSSNLEMIRLAV
                     QFVLEQG"
     gene            complement(13433..15907)
                     /locus_tag="PLS90_01125"
     CDS             complement(13433..15907)
                     /locus_tag="PLS90_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01033.1"
                     /translation="MSQQPSPPEELGTGERDALRQRIQELELRNAGYQQELENLCTAH
                     RHLTEFVNRSDLMALSFDLEWRLLAFTPAAGRLLRLGPGDQGLPLGDLLSRPVGGRLL
                     AGLDHRAPAAVETIVGPVEDRWYSRQVRRFGSTETDQPAAMLIFSDVSALQRRQGDLL
                     ADNETLMSRSDRRAMLLGLQHQIAIAANESETLVELLGLALDRVGRALKWPLGHVYQL
                     DYDTPLLRSSRVWYTDGNRRDPAIEEYRRITAESLITLDEGLIGRVRETGQPLWMDDI
                     RAEKNFSRRIPEGFPYPAVIGFPIAIGRHVVAVIEFYNDAPFRPGQRLLETMDNIGLV
                     LGRAYERDRTRRQLRESRQFIENIADTAPFMVYVYDLIERRNVYVNSQITQMLGYSPG
                     FFAMQGSRLIESVVHPDDQTRVSFSGQELRRMLEGERVDTEFRVRHADGGWRWLRRRA
                     VAFMRTEEGRLRSILAAVQDVTDWKMAEDGLRESEMRFRAIFEQSPLAITLATTDGRF
                     VRVNAAAERMFGYPHEELLGLTFLDLTYPDDREAGVGKYRQLAQGEIPSFSVEKRYRR
                     KDGRIIWGNVFVKLIRDQHGEPRFTLGMVKDITERKQAEEELRHLQKEILELSVREQR
                     RLGQELHDGLGQQLTGLGLIARTLARKLQARGLPEADTMEELVEGLAQSLSQVRGMSK
                     GLLPVEIDAAEFVKALRDALGIIEKRAGIRCKLHTDGAVRLEDNGIATNLFKICQESL
                     INAVKHARPRNIEIEISHEQEELIVRISDDGTGMPEAKDLILGGGLNIMRYRANEIGA
                     QLEIRPAKPTGTVITLRLKGRHFDGA"
     gene            16395..18221
                     /locus_tag="PLS90_01130"
     CDS             16395..18221
                     /locus_tag="PLS90_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK01034.1"
                     /translation="MDTPLPRPEYPRPQFIRQQWLCLNGAWQFEIDPGDSGLERGLLE
                     RPLARQILLPFCPESPLSGIGHTDHLGAVWYRRELRIPPEWADARVLLHFQAIDYDAT
                     VWVNGRELRRHRGGFTPITCDLTGVAAPGDPVTIVVRARDDHRALKPKGKQSHLYRNM
                     GCHYTRTTGIWQTVWMEPVHPGSALLRPRITPDVANGCFLLEQPIDRPAAGLRLRATL
                     SDDQGVLLTREVPADHDFSPRLILPVPQPRRRLWSPADPFLYDLRLELLDAAGAVIDQ
                     AASYAGLRGVTLDGNAIRINGDVVFQRLVLDQGYYPDGILTAPSDAALARDIELAQAA
                     GFNGARLHQKVFEERFLYHADRLGYLVWGEFPDWGCHDGQRHEENQRPDAAYITQWLE
                     VLARDYSHPAIIGWCPLNETAEPIIDKLTALDDVTRGMFLAAKAMDQTRPVLDASGYS
                     HRVPEADVYDSHDYTQDPAEFAERHAHTAAGRPFVNEPGGRPASIPWRGQPCFVSEFG
                     GIWWNPDATPGEHSWGYGDAPRTIGEFYARFRGLCEALLRNPALFGYCYTQLTDVYQE
                     QNGIYTFDRRCKFDLDRIRAIQQLPAAIEQAHRPTRPDDSSS"
     gene            complement(18306..19751)
                     /locus_tag="PLS90_01135"
     CDS             complement(18306..19751)
                     /locus_tag="PLS90_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPK01035.1"
                     /translation="MASMRAGLDFALLKPDWLWLLRVWGLVLLALHVKSAAAQKAFPG
                     AEGAGADTRGGRGGQVLFVTNLNNAGPGSFREAVETHGPRTVIFHVSGIIDLSSSLTI
                     DKPYLTVAGQTAPGDGICFRGNQITIRTHDVILRHLRFRPGDIGGAVPQDWPGDALAL
                     EGARNVIVDHCSFSWAVDECVQIYAGGPDDEDTTHITFQWNIISEPLRDSTHAEGPHG
                     ANLLIRTDGGDVSIHHNLLAHGDTRNPRVGSDTDDPTHRTRLDFRNNIIYNWGDVNGA
                     GGGRREWADINYIGNWVQTGPSTTDDVDIIFKSGVGANHPTSDNYRIYQVDNVLDGLD
                     IGWNAFVDRYTQLTMPVEMPITITQAPGEARDLVLAEAGATLPRRDPIDERIINNVVA
                     GTGEIIDSQDEAGGWPIYGAIAPPPDSDLDGMPDHWETENQLNAADPSDSTEDADGDG
                     YTNLEEFLNGTDPQRDEHNDTGVGRKTWMNY"
     gene            complement(19963..20460)
                     /locus_tag="PLS90_01140"
     CDS             complement(19963..20460)
                     /locus_tag="PLS90_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPK01036.1"
                     /translation="MRSGDGEYSDQNAGRGAGGERRFRLSPAPLERDGLRAALADPRA
                     GGFVAFEGWVRDHNAGREVVRLEYEAYGALAEREGERILEAARERFEILDARCVHRVG
                     ALGIGDLAVWVGVSAVHRGPAFAACQFIIDEVKHRLPIWKKEFYPEGDSGWVNCEHCA
                     RAPHH"
     gene            complement(20467..20724)
                     /gene="moaD"
                     /locus_tag="PLS90_01145"
     CDS             complement(20467..20724)
                     /gene="moaD"
                     /locus_tag="PLS90_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HPK01037.1"
                     /translation="MPATQSLNVEVQYFAILREQRGLAREQLATGAATAGELYRDLAA
                     RHALALGPEHLRVAINDEFSAWDAPLAEGDRIVFIPPVAGG"
     gene            complement(20730..21779)
                     /gene="moaA"
                     /locus_tag="PLS90_01150"
     CDS             complement(20730..21779)
                     /gene="moaA"
                     /locus_tag="PLS90_01150"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPK01038.1"
                     /translation="MQADRPLTPAALQPVRPPLIDTFGRRHTYLRVSITERCNLRCLY
                     CMPAAGIPLKPREDLLTFEEIIRLVRVFARLGIDKVRLTGGEPLVRRGVEGLVRELAA
                     IAGIGTVAMSTNGVLLAEKAAELRAAGLSRLNISLDTLRPERFERIALRRNFEQVMGG
                     IEAALAAGFAPLKLNCVVMRGINDDELTDFVEFARTRPIQMRLIEFMPFHGNAWSGDK
                     LVSYGEMRTALERDYRLEPAAGEDGASNVAREFTVTDRRGRRLAGSVGFITSMTRDFC
                     GACSRLRLTADGSIKSCLLHPAEASLRDALRAGAGDEELERRIRRAVSLKPYGHEPMG
                     AIDAERNRSMTAIGG"
     gene            22381..24012
                     /locus_tag="PLS90_01155"
     CDS             22381..24012
                     /locus_tag="PLS90_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01039.1"
                     /translation="MMSSVRFRLTCALLLLATVASAETRKMVAFPAATFTFGAPVVNT
                     NGGVFVATSDVNIYFQDGSSAEPNRIFGAGDSVAGGTFLSLNNFRPISPIFGGDGVLA
                     LGSYELEDSGSQQILIALDADGHLLGQVLASDAAFSEINNPVIGLPAAVHLHRTGKAG
                     VNDLGIFGAVGPQPAPPDEPFQLFHVGFIEGNFTPMLVDTPYFPPQSGANGFNNSFFR
                     YQGPLLGGTNGASDLFQLTGPIDPSIDLPAPEGLNPDDVAAFGLLIRPEVEGVAAWSR
                     ELLRRVNDADDPGTPPDAEPIFTITFNFEAFNLTRHTVIFAGNVQQTAPEMGGEEQAG
                     GLASDTLDTTDENSDCDCSQADLDGDGDVDRKDVRIARKCYLEGGGDQCKDVNGNGVA
                     SELRDWECVQYCATQEGGGNKDLINPGSAVLLLRQGQPLYFEPSLTLQGFNAVRGNGI
                     GAGAFLVSVSGPQGFSRALVGIDADASAPKVLVKTGDDLGDGPITDIQNLSNINELGQ
                     VAFTASVSGAPGPIVYRTDIVALPENAADRWEVYF"
     gene            complement(24298..25356)
                     /locus_tag="PLS90_01160"
     CDS             complement(24298..25356)
                     /locus_tag="PLS90_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK01040.1"
                     /translation="MTQSTPPAAEQGGAGLEGRLEALGALFEEALERFVAARSAQPIE
                     NLHEGLRYALGLDLPAGPGRGKRVRPLLCLLTAEALGADPRRALPFALAIELMHNFAL
                     VHDDMEDGDVMRRGRDAAWVRYGAAHGINLGDYLLVQMMAALAEGEPDGLDDATRVRL
                     LRLLTSALDHTHVGQALDINYRHRRSLSEEEYLRIVREKTGYYLAAPIQGGAIVAGAS
                     QRVIETIGEMAQYLGPLFQIIDDIIDLTDGKGRESVGSDIREGKRSYLVAYAAQHGAP
                     AQRERLFDILDKPREATTRADIDEAFAIFEALGAVAAGRDYCRRLHEDARRHLERLPR
                     ELAEPLGAVIEMLVSRKQ"
     gene            complement(25353..26222)
                     /locus_tag="PLS90_01165"
     CDS             complement(25353..26222)
                     /locus_tag="PLS90_01165"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014436836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPK01041.1"
                     /translation="MAYDITLTQAALLGVVEGLTEYLPVSSTGHLIIVSALLGIPASD
                     GAKAFEVVIQAGAVAAVAAIYLPSLRAMLAGLLGRDGGGRALLGQLVTAFLPAAVVGV
                     VANDFIKAKLFSPGPVVAALAVGGALMIAVERVRSRRAGGAAAARQAGLPMEAMTLRA
                     ALLIGVAQCLAMWPGTSRSMVTIVAALLLGYSPRAAAEFSFLLALPTLGAATCHDLLG
                     EGGAILEAAGPAGLALGFLTSLVVAWAAVKGFVAYLTRHGLSPFGWYRVALAVLLLGL
                     TLAGIIQWEELMQ"
     gene            complement(26745..27320)
                     /locus_tag="PLS90_01170"
     CDS             complement(26745..27320)
                     /locus_tag="PLS90_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK01042.1"
                     /translation="MRHRPLHRAWSVRGLTYGEMLIVLAVFSVLAVIFVFSSKHAMVK
                     TRLSATIQTQRNFSSEFEAFRAYTGRLPGETTWVRELSQPAGGGHYVPDDPFVPDGAE
                     ERKFEFYKDIGPATAWVLVSVGPDGDSDLKEAIRQIRSEDHPTLVSAESAGFPMGGPL
                     TARELRELVERYSYDPTNGTISDGDVIYVSY"
     gene            complement(27900..30173)
                     /locus_tag="PLS90_01175"
     CDS             complement(27900..30173)
                     /locus_tag="PLS90_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01043.1"
                     /translation="MAEHRTAEEWLEILRGVTRERLARECRERIVTQDLKTAAQRAAA
                     LLQRARGERELEDHARFLGRALGELHRDVDRSRKVIAALGRAGGQAGQGAAALAASFE
                     EFVAQGGPRQFEATSQAWYQQLTEAVLSLRDCLPGPNEAGEPTEEQIARFRDELLRIL
                     KAGAHQRDPDDFIDALSIVVEYCPKDPSPFEGHAGLGDRMFLQLGPRAKLTAVRTMVQ
                     LGELDNLRGAILKFADTADAAGRAKLLTGIMGGMRHADFFPWLKRGFEKTSSDLEESW
                     FIDALGRINEPRAAEILLERLEKVVKRLGEKGAARRAELLITALGRVARLRGIDPDRR
                     NQVIQKVLATVERGDRALVFKATAEMFSVRLQELDPQLLRWAAQHAVDAMWGPPLDVQ
                     GTRISVNGWREPMVETLRRLGRNALDEILEAGRKHAAHYSAAMAALANALQTIGDKRS
                     VPLLEAMIRTALFHDEAGPRSQVLEEKVLDAASGELVELNRDDLIHTLIYTLLESGGE
                     AGIQAALDLADQMQAGQIRIPGQQTAALLMDTKMKHGGLGRVSRRAEVLELDEKELKH
                     ALSQAKGGMLVKKPTQIQALATLGRTRRPEAAEVLLELFGDKDPVIANAAQTALAQFF
                     QPLPEEAEFTRTIEALLGQPRQLKGARLERLLDFIRRTLPKRPPYNRLYERQIEILIE
                     DAELAHRLRGAALATDLPASPAGGEEGDGGAGEKPGASALPAAPISELDKKRAYLQAR
                     KAWIAGGKKGDEPLPPE"
     gene            30351..31538
                     /locus_tag="PLS90_01180"
     CDS             30351..31538
                     /locus_tag="PLS90_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPK01044.1"
                     /translation="MPAPPPHGLIYLDHSATTPIDPEIAARMRDADARFPGNPSSIHA
                     AGRAARLCLEEARSALAGSLGMPAESVIFTGSGSEANNLAIAGTLLEHAGRPAHLIVS
                     AIEHHSVLHCARALAARHDGVTLTEIACGADGRIDLDAIADAIRPATRLISLMLVNNE
                     TGVIQPVAEAAHRARDRGIRLHVDAVQALGRLPLDLPALGADLLTLAAHKARGPRGVG
                     ALLVRPGCGLAPLIHGGTQENYRRAGTENLAGAVGMAEAVRRAVAATDAHRRHLARLE
                     RIFLDTLTGQAAAGPRFELNGCATAKVPGILNLAFEGVRQDDLVVGMDLAGVAISAGS
                     ACSSGVIEPSHVLAAMNLPPWRRDGGVRISFGSTNTEQEARQAAARLAELARRLATST
                     AHG"
     gene            31649..32269
                     /locus_tag="PLS90_01185"
     CDS             31649..32269
                     /locus_tag="PLS90_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01045.1"
                     /translation="MARYLLCFHHQTDPAQALPWQEPLEQFPCSLLRTKTLHEAKTYC
                     RAYPLVMALVRFHERTPELVDAVRDLAAAIPEGKRLALIGLSDQPLAAADRAELCAAG
                     LCDFLAAEDPPAIVLWRLEILSLLADLDQFAKARMDVGELAKKTRERLHDMSQPLTAV
                     QGRLQLLAAKATPEDPNAEYYREMVRLMAAATRQIAEMQQLHRAFS"
     gene            32694..37331
                     /locus_tag="PLS90_01190"
     CDS             32694..37331
                     /locus_tag="PLS90_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPK01046.1"
                     /translation="MSAQASEPTPAPPSRAQAAPRPPRRRWPRILALLALILVILALA
                     LVLYAQSSHALRHVYLPLINKNLNGELSAQGGSASLRGRLHLRQPVIRDLGGNAIFEA
                     RELVVNVAPLSLLRRNEAPRVNELRLASPRIVVQVEPDGHLNWEKLTKGAEPVVEPQH
                     EPDDEPDRLPAVEIRKIEISDLDLTYRDRDGLEGRLEKTSLNIANLAPGETAAINLDL
                     NGSVRQPDAGIDQRTRLTLNGEVTQDDDRDGLGWELNGELRSEGASPAAPEPVTVQMT
                     AATAGNYSLTGDWTMRFEIAADTPEGSVGRFTGDAQWARTAGSRQAHVEFSQISREFL
                     NPLLAAAGMQLEQAALDGRIDVEGSADALAFDSTINAANLSLRVGAGKPTPRVDVAFR
                     QAGSLGFEPRQVNWREMLLTVDEGGRRVVEVTLDQPLVLDLARAQADAGGALAGETAQ
                     VQLNLNDFSLDELGPWLAALGSAPSDQVPSGTINADLTLRVAQLGGRIDVTGAVRGGA
                     LRGGPLGDLPFDLNADLAAELTNQEQLAIRQADINLRQEDAELAHAAVSGQLNLTAQD
                     GQLALVAELPRFARAMRSLGYLPAQSPLSESGPLRADVDLALLGPGAFRLTGDVQGDN
                     LQVTNLGGGPLNLAARLNAERDAKGLIQFEQTRLVVSEGAAALATMTLAGGINPTEQA
                     GQVSFQADLPQAVRAARMLGVLPADSPLAGSGPVTADLKIAFSGKQRLQVEGMVAADD
                     LLLAAGSGGPLDLNARLKGTRDPEGLLQFEDTRLTLSEGEAQLASVRVTGGVNPAAFT
                     GQVEIEAEAPRLLTALQRAGLIDPSATDGVGDGKLTAAQSINLRQDGTIHVTGRAAAE
                     GMSVSLRGSPPLELGARLQNDASLNPRTRELSLAPLRLELQQPGGQPGVIEARGDWSL
                     AAAGGGERRMELTITDVDLAPWLRLTRPMRVDDLPPIPLNARETLIADAQGAVQILGE
                     LHIGLPSSAESAALDVTVRNELAQVGDQIQRLIVELTSRANGQPADSARIEGAGTLGD
                     APDLQLTANIERFNANPYLALADRLSGDAEAPSGGTASAKTTGAGEASSTAAAPPNLT
                     LHADFTIARAEAREAVLTDASGRVEMADGVARIFLDEGQVNGGPIRGDALLAMAGPEP
                     RYEWNMAVEEARVESLVDSFAPRLKGRLTGTLDASSAGSARGSGEALKRQLQGEATFS
                     IADGQIAGLELLELLARETRIPSFESFKFFSFEGDVHLRQGRAELDGVRIVGPSHALA
                     IEGFYGLDGQLDLSIQPAVAADLIPLETKNEYLQALLAPGETGLTPLPLKFGIVGSTG
                     NYSVRPEVALPASREQLGNTLRGIVGGALQDRLAGRGDPDDGEAGGEKRESLLPELPE
                     LPGLGGLTGRRDARRAIRAGDPSEPAPADETTGPAPASDEAPQETGAAETEPTPAPPE
                     PSGDAEAPAQSRDEGDKAPAETPPPAPTPEPEMTPAPTPEPTPAPAAEPSPQPVPTAE
                     PEVTPGPQAQPTPQPEAEPGPEPTPQPTPAEAPDEPAPPPAPENDDAATTG"
     assembly_gap    38075..38174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(38431..38694)
                     /locus_tag="PLS90_01195"
     CDS             complement(38431..38694)
                     /locus_tag="PLS90_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPK01047.1"
                     /translation="MADFQLQIYTQVRKVFEGRVESIIVPAETGYLGVLAHHAPLLAT
                     LGDGKLTVRDHMGERYYHVTGGFLEVRDNVATLLVDSLEDIAA"
     gene            complement(38767..40188)
                     /gene="atpD"
                     /locus_tag="PLS90_01200"
     CDS             complement(38767..40188)
                     /gene="atpD"
                     /locus_tag="PLS90_01200"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPK01048.1"
                     /translation="MNIGKIKQVIGPTVDVEFHSDRLPDIYNSIHIENKHSGKRLVVE
                     VAQHVGNNTVRCVALGPTDGLVRGMEAVDTGAPIKVPVGDQVLGHIFNLLGDPLDGRG
                     ELPHPEQRWPIHRPAPELTDTLPATQILETGIKVIDLLAPYAKGGKIGLFGGAGVGKT
                     VIIMELINNIAKQHGGYSVFGGVGERTREGNDLWLEMNESGVVSHEAGKSKAALVFGQ
                     MNEPPGSRLRVALSALTMTEYFRDEMGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAV
                     GYQPTLATEMGALQERITSTNKGSITSVQAVYVPADDLTDPAPANTFAHLDATTVLSR
                     QIAELGIYPAVDPLDSTSRILDPMVVGAEAYQVARAVQNTLQRYKDLQDIIAILGMDE
                     LSEDDKLTVARARRIQRFLSQPFHVAEAFTGRPGAYVKREDTVRGFKMIVDGELDELP
                     EQAFYMAGGIEEVMANAERMAKV"
     assembly_gap    40315..40414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(41007..41900)
                     /gene="atpG"
                     /locus_tag="PLS90_01205"
     CDS             complement(41007..41900)
                     /gene="atpG"
                     /locus_tag="PLS90_01205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPK01049.1"
                     /translation="MAESLKVLRRRVRSIKNIKQITRAMEMVSAAKLRRAQATLMAAR
                     PYAAKLQELLAHVASSSVIEEHPLFQPREGNRKTLVLFTADRGLAGSFNTNLIKKTEE
                     LLRGDPAAKWELVLVGRRGRDYFQRRSWPVARSWIGLVGQADDGLARELANYLLERFL
                     RDETDQILLVFSSFISTVVYRPTVAQYLPLTPEALGIRLDEAGQAEARQEVDYILDPS
                     ADIVFDSLLPRYLSSKIYITLAEMATSEHSARMISMNNATKNCNDLQDSLQLRLNNAR
                     QAAITKELLDIVGGAEALKTG"
     gene            complement(41905..43572)
                     /gene="atpA"
                     /locus_tag="PLS90_01210"
     CDS             complement(41905..43572)
                     /gene="atpA"
                     /locus_tag="PLS90_01210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPK01050.1"
                     /translation="MALRPDEITEILERELTGYQAGIDVENVGTILKVGDAIATVYGL
                     HDAMAGELLEFPGGVMGMVLNLEESSVGVALFDPGIGIKEGDTVKRTGRITSVPVGDA
                     MIGRVVNPIGQPIDDKGPIPTDSYRALEVIAPGVVERQPVKQPLMTGLKAIDAMIPIG
                     RGQRELIIGDRQTGKTSIAVDAIINQQGKDVICIYVAIGQKASSVAGVLKTFRDHGAM
                     DYTIVVAAMADDPATMLYVAPYAGCAIAEDFMWKGKHVLVVYDDLSKQAAAYRQLSLL
                     LRRPPGREAYPGDVFYAHSRLLERACKLEEKYCIVRKGETADASKGGVDGKVYRGTEG
                     GEEAARKALEAMADKEQLEVRMDPTTGGSITALPIIETQAGDVSAYIPTNVISITDGQ
                     IYLEPDLFYSGQRPAVNVGLSVSRVGGNAQTKAMKKVGGQLRLDLAQYRELAAFAQFG
                     SDLDRATQQQLTRGARVQEVLKQGLNQPQAQSYQICILYAATQGHLDDLPLEALARFE
                     QELNEYIHEQYHDIHHDLDSKGVLGAELEEKLKRAVLEFKQKFEAKG"
     gene            complement(43788..44363)
                     /gene="atpH"
                     /locus_tag="PLS90_01215"
     CDS             complement(43788..44363)
                     /gene="atpH"
                     /locus_tag="PLS90_01215"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPK01051.1"
                     /translation="MIRQRVYNPVSRSYATALFNAAQRQGTLQRLLEECRVLREIAGR
                     TPQLRVFFENPRVPTEDKQALIDKVFGGRLSPLLIKLFHLLIERDRSLLLDEILDLFR
                     ELVEEAEGIQHAIVETARELNFQEKVRLKSSLEKFTGSKLRIDYAVDPSLLGGIVFRY
                     QDTLIDSSLRAGLREIRRRLAGATVLRDEAA"
     gene            complement(44360..>44751)
                     /locus_tag="PLS90_01220"
     CDS             complement(44360..>44751)
                     /locus_tag="PLS90_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPK01052.1"
                     /translation="LDERQRKIEEGFADIERRQQEAQAALDEYRQRLEGIEQEARQKI
                     QEAIAEGRRVAAELTENAREEARQITERAQRNIELQIGQARLELRDEVVQLVMDASER
                     LLREKLDEQTDQRLVAAFIDDLERQRA"
BASE COUNT         7242 a        16962 c        12734 g         7613 t
ORIGIN      
        1 ccgttgcaga cctggcaccc ggctgaaatc gtagaccgtc cccccaacat ccacttgtac
       61 cctgtgccgt tgcagacctg gcacccggcc ccctgataga acggctggtt cgccttggcc
      121 ggaatcctga tcgcatcgag cagttcgggc gacggcgtat actcctcctt gcattcgttg
      181 cagatgcgcc gcaccagccg ctgcgccacg atcatgttga gcgacgcggt caccaggaac
      241 ggctccaccc ccatgttcgt caggcggttg accgtgctcg gcgcgtcgtt ggtgtgaatc
      301 gtcgacagga ccaggtggcc cgtcagcgcc gccttgacgg cgatctccgc cgtctccagg
      361 tcacgaatct cgccgaccat gatgatgtcg gggtcctgac gcaggaaact gcgcagcccg
      421 gccgcgaacg tcagcccgat atcgggccgc gccgccacct ggttgatccc ctgcaactga
      481 tactcgatcg ggtcctcgat cgtgctgatg ttcttgctcg ggtcattgat cgtcgacagc
      541 gccgagtaga gcgtcgtcga tttacccgaa ccggtcggcc cggtcaccag caccatcccc
      601 cacggtttgc ggatctcgct ctcgaacttg cgcagcaccg gcgcctcaaa ccccagatcc
      661 gtcaggcgca cctgcagatt gctctggtcc agcacacgga tcacgacctt ctcgccgtgc
      721 gcgaccgggc aacagctcac acgaaagtcc accgacttgt tctgctggcg caccttgaac
      781 cggccgtcct gcggcagccg cttctccgcg atgtccagat ccgacagaat cttgatgcgc
      841 gagatgatcg cattctgaaa cttcttgggc ggcggcggca tctcctgcag cacgccgtcg
      901 atccggtagc gcagccgcag gctgcgctcg tacggctcca cgtggatgtc gctggcgccc
      961 atcttcagcg cttcggcgat catcaggttg acgagctgaa ccaccggggc gtcgtccgcc
     1021 tccaggctca ggtcgtcgtc ctcctccgcc tgcaccgcct ggcgctcttc ctcaaggacc
     1081 tccaactcct ggtcggtgat gtccttcagc gcctcgtgga aattctcttc ctggtccttc
     1141 ttgtaatact tgccgatcgc ttcctggatg tccgactcaa agctgatcac cgggatgatc
     1201 tggcaatgcg tcagcagctt cagttcatcg agcagataaa tgttggacgg atcagccagc
     1261 gcgatcgtca gcgtgtcgcc tgtcttgtcc accggaatga tcttgtaccg gaagaccagg
     1321 tcgcgcggga tgctttccag aacttcggga tcgatttcgt aatgcgacag cgtgacgaag
     1381 ggaacgttca gttgcttgcc gagcgtcgcc gccacctccc actccgaggc ccggcccatg
     1441 ttgatcagga tccggcccag actcaggccg gtctccttct gcttgtgcat ggcgatcttc
     1501 agatcatctt ccgtgatgat ctgatcctcg atcaacaact caccgaggcg tttctccatt
     1561 gcggttggca tggcggctcg cgatgctccc caactgcgcg ccgcccgctc cggatggccg
     1621 cgcaatccca ggcaaaatcg acctcctcgc cctggcgaaa ggtctcactt cattcaacgg
     1681 acgatcgcag ccagaacatt agttggctca aggaggaatg tgtgcggcct gccccctcac
     1741 gatagagaca taagggtagg aaccgacata aaggaaatct ccctccgacc cgccgccacc
     1801 ggtccaacga cccgctcttc tccgcatccc ggccgcaagg tccctggcct tggccatttc
     1861 gactccgaca tcccgacagc gaggcccctg gagcttgagt atttcgacgc tcggcctccc
     1921 gtcgcgatgc cagacagtag ccggtggtca agcgcagcga caccactggt cacccgatgt
     1981 ccccaccccc gcatcccggc gggatgcggg aaaactccga atttccccct gcctcacagc
     2041 ctgatccgcc cggcctccgc cgccccctgc ggaatcgccc gcagcaggct caccaggttc
     2101 tcctgcgccc gcccgcgcat cgatccgcag tgccggcgca ggaacgcgag cgtcccctcg
     2161 cgatccatct cgggccgtaa ctgatggcgc ctcagcaatc tgcgcagttg gccgggccgc
     2221 gtcgcatatc ccagcgacgc gcagcggtcc tccctgaagg tcttcatcgg ctccccgtgc
     2281 gggttgtaat agatcaccgg gcgtcccgcc gccagcgcct cgtacggcag gttgctgaac
     2341 cgcgtcacga ccaggcttgc ctccgcgatc tgcgccgcca gccgttcggc cgccgaccgc
     2401 accaccggtt ccccctccca gctcgaagga tcccgcgggt gctgcgaaat gaaatagtcc
     2461 agccccaact cccggcacgc ccccagcaca tcgtgcatcc acagcgcccg ggcttcctca
     2521 tattgtccgt acgtgaagtt gcagttgatc aggatccggg gccgctccgg cagcgcgatc
     2581 ggctcgaacc ggtcaacctt cggattgccc gtcacggcga agcaccgcgg ccggatgtgg
     2641 cgcagcgtca ccgccccctg cgccagcacg atgtcggcgt tcaggtactt gcgcggattc
     2701 cggccccccc actccgcttc ccagtcctgc ggtccctcct gcagcgcgac gctcggaatc
     2761 cccagccgcc ggcagagccg ccgggcgtgc tggaactcct ccgaccagtc gttgccgcac
     2821 agcatcaacc gcgggccgaa ggcgcgcacc agttcctcga ccccgggcgt cagctccacg
     2881 aacgcgaatc cctcggactg gagcgtctcg gccgccccct cgtccctcag cccccgcggc
     2941 gggatcagaa acagcacgtc gcccagtccc tcggccgccg gaatcatgaa tcgcgcatgc
     3001 gtatcattgg agacgaggat cagcagccgc ggatgcgccc cgcgcggctc gaccctccgc
     3061 acgaagtccg gaatcgccgc cagctggagc cggactcccg cctccgccag ttcctcgccc
     3121 agcgactgtt ccaccacggc gcgctcgaac gactcggccg cctcgcgcag cgaaaaccgc
     3181 tcgttccgcc cctgcgcggc cgtcgtcaac gcgaagtgca gcccggcggg cagcgccggc
     3241 gtcgcgcacg gcgcaaacgg acggcagatc accagggttc cggatggagg cagcgccatg
     3301 gatcagtccg acaccagcac ggcgcggcac ggcgatccat cggcgtcgat cagcttgagc
     3361 ggcaacgcga tcagcgtata ccagcccgcg ccgacggcgc gcagatcgat cccctccagg
     3421 atgaggacgc cggccccgag cagcgccgtg tgcgccggga aatcgtccgc cccgaaccgg
     3481 tcgatgctgg gcgtatcgat cccgaccaga ttgacccccc ggcggaccag ttcctcggcc
     3541 gccgcgcccg acaggccccc gtcgggttcg tccagccggc ccgtctcata ctgatccgca
     3601 gcggccgtct gaaacaggat gttccgcccg cgcagttcgt cgcccggcag gtcctccggt
     3661 tcgatcaccc cccgcggcgc cgcaacgacc agcgccggcc cgatgaaccg ctccaccggc
     3721 agctggtcca gcgtcgcgcc ggccgcgacg aaatggcgcg gcgcgtccag atgcgtcagc
     3781 gtgtgcgagt tcatgtggag catccgcata ttgcagcggg ccccgtcgac gccgatcgtg
     3841 gagagcggtt cgagcgcaat cggcgggtcg cccgggtaaa caaacccgcc gctcgcgatc
     3901 gtgcgggaga tgtcgtgaat ccgccgggtc atcggccttg cctccggacg cgcctgcaag
     3961 cggtcgacaa actatagggc ctcccccgcc catatgcaaa gcccggccct tcatccagac
     4021 ttcggctccc ggctctcgtc cctcgtcccc ggctctcgaa aaccccggtt tttcttcctt
     4081 tggggttcat tcgtgtcttt cgtgatccat ggcccttgcg acaccccctg tccacaaatg
     4141 gagacaggcc caactcaaat aaaatgaaag ctatgtcctc aaaatggggc catctcgggc
     4201 cttccgcccg ccggatcgcc ccaccggccg ataaattatc cgtgagcccg cgtggagtgg
     4261 acggaagaac agggaagaaa cgacaaaaat tcacgggaac gacgtcgccc gcaaccgctc
     4321 aggctgcatc cggcgcttcc cctgttttcc ctgatgtcgt tgatgtcccc tttctccctt
     4381 ttatcccttc acgatttttc tccgccgtca aatttggggg gttaacacgt cgcccaaacg
     4441 atgttacgat ccgagtagct gagcatggtc catctggagt ttacgcatct gagattattc
     4501 aacttggttc caacgtcagc cgtttcccgg cgcctcctgg cgttgtgcgg cttcgttttc
     4561 ctcagcgccc cgctccccgc cctgggcccc aacgtgctgg tcaacggcgg atttgagaat
     4621 ccctaccccg acgatgttta tgcccccgat gagcgccccg agggctggag cctcgaggat
     4681 ggcaacgacg accagtttct ccgcggccgc gaatacgtct ggatcgatgg cgttctcatg
     4741 ccccaggaaa cggtcaagga ggggcgccgc tgcatcaaaa tccaggggac ctggcacggc
     4801 gacgagaccg gaccgcgcat ctaccacgac aaactcgccg tcgcccgcgg actcgacaac
     4861 gacgcctact acctcctcca gttctggtat cgcacctccg gcatcgaacg cgccgatttc
     4921 ttcgttcgcg tcaatcatta caccaactac cccgaaaacc cgcagacccg cccctcctct
     4981 ttcgccgaat ccgaaaacga cctcctcccg gccgaggatt ggacccccgt ctcattcctg
     5041 atccgcccga ttcaggaaga tcaggccccc tacgcctacc tccccgacaa gtaccccaat
     5101 ttccgcggca ccgatgcgct gcgcatcatg ttcggcctga ataacaccag cggctgcgtc
     5161 tacatcgatg acgtgatgct gcgcaagatg gatcagaacg gcagcgaagc ccacatgatc
     5221 ccgctcaatt ccaaccaggt cgatcccccc gcggtcgacg gcctcaagcc ggtcttcccc
     5281 cccaccgatt tctacacgat ccagcgcgac gaacgcggcg tctggtggat cgtccgcccc
     5341 tcgggcgatc cggtctggat catcggcatg aataatcccg aggatcccgg cagccgcaac
     5401 cccgccatgt cgcaatggat tcagcagcac ttcgcgccgg gcacctatgg cgacgacgtc
     5461 gtccgccgcc tcaaggcctg gaatttcacc gccaccatcg aaaacgacac ccccggctcc
     5521 gcccgcaact ggaaggccga gcagcgctac atggacttca gcggcgacag ctgggtcagc
     5581 aagagctggc gcatgcgcga ggccgacggc ggctattggg gcgacgacgg cgcctatctg
     5641 gccgatccct acaaccccga ctggcaggcg cgcgcccgcc aggaagtggc cagggatctg
     5701 accgccagcc gactcgacga ccccgaaatg ctcggctact acaccgacaa ctgcctgccc
     5761 tgcaagaacc tccacctggg catctggtcg actcactgca gtcaggtgtt cctcaactgg
     5821 ctggcgacgc gctatcccac cccgcaggcg tgcgcccagg cctggtcatc caaatacaag
     5881 acgttcaatt tctcctcctg gaaccatgtt tatgacgccc gcgccgacgt gcgaatccac
     5941 ggcttcgacg attcccccgc cctcatccgc gatctgaagg atttcgaact gaaggtctgg
     6001 aaggattact tccgcttcat ctgcaccatc atccgtgaac gcgaggcggc ggtcttcggc
     6061 gccgatccct ccggcatgcc cctcaagaag cacctgatct tcaccaaccg cttcccctgg
     6121 aacggctatc ccgacgccgg ccaggaaggc gtcgagcgca tgatggacgc catccgcgaa
     6181 ctcaatcagg agcgccccgg cttctacttc gatgccctgg cggccaacat ctatcccggt
     6241 ggaatgcacc tgcccgacca gaaaagccgt cagaacatgc tctttctgga aaggatgagc
     6301 cagcgctgcg gtctgccggt ctttgtctcg gagttcgcca tcgccggcaa cgacgtcggc
     6361 ttcccccctt ccgtccgctg gcgcgaccgc accgtcccca cccaggccga tcgcgcccgc
     6421 gcctaccgca acctgatgtt tacctggagc cgcctgaact tcatgctcgg cggcatgtgg
     6481 taccagtggt acgacggcat gtacgacaac ggcgggccga gcaaggccgg cttcaccggc
     6541 atcgatccgg ccgatccgaa atacgacggc cgcaacagcg gcttcgtcga cagccagaac
     6601 cgcgaataca ccgtcttcgt ccagcaggtc gccgaggtca atgcggtcct ccgggatacc
     6661 tcccgctcca atgccgtctc cgttgccgac ttcccctggg acaccagcgg cggccaggcg
     6721 cccccgcccc cccgcgaacc acgcctcatc caccattccc tcagcggcaa gacctatctg
     6781 acctggggcc acaccgacga tcgcgacatc tggaactacg tcatccacta cagcccccgg
     6841 cccaactgcg actacacccg cctggcttgg ctcggctacg tgaccagctt cgaaatcccc
     6901 gccgacaaac ccggctacta ccgcgttgcg atctccgaca gccagcgcaa cgagggcccc
     6961 tggaccgaag ccttccacac ttcgcaggcg acccccctga gcgacctgac catcctgtcg
     7021 cccgccagcg gcgaggatgt cgccttcggc gaaccgatcg acatccgctg gctccccccg
     7081 gcgccgaccc aacaggtcct cgtccagatc cagcacctcg tcgacggtca gtggcaggtc
     7141 ttgcagcagt ccgagtggat ggacaacctg ggcgagcacc gcgagtggat ctggcaggaa
     7201 ctgcccgacg gcacccagct ccgcgcggtc gtcaccggcg gcaactggga cggctccagc
     7261 ttcaccgccg ccagcgcccc gttcactttc gacaacccga agaccgagca gccgcgcacc
     7321 gccgcgcgcc actggatgta ttactgatcc tgctcgatcc cgaccgcctg gcacggcggc
     7381 aactcgatct cgaacagcgc gccccccagc cgcgcggatt cgcccacgtg aatccggccg
     7441 ccatggagct cggcgcacga ccgcaccgac aacagcccca gcccggcccc gctttccttc
     7501 gatgtgtaga acgcgtcgaa gatcgcctgg cgcttgtccg gcgggatccc cggcccgctg
     7561 tcatgcaccc gcagcaccac ccccggcgcg cgctccttca gctcaaccag gatccgaccc
     7621 tcgcctccca tcgcgtccgc cgcgttcaac agcagattca gcagcatctg ctgcaacgcc
     7681 accggatcgg aaacgaaccc cagcacctct ggccccgcgt actccagcgc gcagcgcctc
     7741 acccgcttgt ggatccgcgc gaactccagc gtatcgcgca cacacgccgc cagatccagc
     7801 ccccgcggac ggtactgcga ccggtcgctg ccgatcgtca tcagccggcg ggagagcttc
     7861 atcagatcgc gcacgcaccc gttgacccgc gctccgacct gctcgggcgt ctccggcccc
     7921 tggagcgcgc cccgcgccag ctccagactg tcgacgtcgg tcaccatcag cagattgttc
     7981 atgtcgtgcg cgatcgacga ggccagcgcc ccggccagcg cgcgccggcc agccgtcagc
     8041 agcgcctgcc ggttgcgctc gatttccagc tcccccagcg aaatgcgccg cagcaggtaa
     8101 tacgcgatcc cgaagaacac caggctggag acgacgatga acgccaaacc cttgagctgc
     8161 tccaggcgct ccagttccac cagcgattcg gcgaaccgcg cggccagcgt cgtcgaaacg
     8221 cgaatgtaca gcgcgcagac cgccagatac gacaacgcca gcaccgccgc cagccgcagc
     8281 ggccgattgg ccggcaacag cccgccgcgc aacgtcgaac ggggaggcca tcggatcgct
     8341 cccatcgcct ggttggacct gcctttctcc tgaccggctt gcgggcggct ttccgccgca
     8401 agccccttcg cgccacttcg gaccgcttgt tacttcacgg ctcgcgggga ttttactcat
     8461 cgttccgttc gtgcgcaata cgatacctga ctccttttta tccagaaaac cgggtccgcg
     8521 tcagtctgcg ccgctccgcg tctttgcgtc ctcggcgtga aatcatcgcc cccaacggtc
     8581 cgcccgaaaa ttcggctctc ggctctcgtc cctcgtcctc ggctctcgaa aaccggccat
     8641 accattcccc cccttgtgca cctccggttc atggcaacgc gtccctcgtc ttggcgggtg
     8701 attttttatc tcagtctgcg ccgctccgcg tctttgcgtc ctcggcgtga gatcatcgct
     8761 cccaacggtc cgcccgaaaa ttcggctctc ggctctcgtc cctcgtcctc ggctctcgaa
     8821 aaccggccat accattcccc ctcttgtgca cctccggttc atggcaacgc gtccctcgtc
     8881 atggcgggtg attttttatc tcagtctgcg ccgctccgcg tctttgcgtc ctcagcgtga
     8941 gatcatcgcc cccagcggtc cgcccgaaaa ttcggctctc ggctcacgtc cctcgtcctc
     9001 ggctctcgaa aaccggccat accattcccc cccttgtgca cctccggttc atggcaacgc
     9061 gtccctcgtc ttggcgggtg attttttatc tcagtctgcg ccgctccgcg tctttgcgtc
     9121 ctcggcgtga gatcatcgct cccaacggtc cgcccgaaaa attcggctct cggctctcga
     9181 aaaccggcca taccattcct ccccttgtat acctccggtt catggcaacg cgtccctcgt
     9241 catggcgggt gatttttcat ctcagtctgc gccgctccgc gtctttgcgt cctcggcgtg
     9301 agatcatcgc ccccaacggt ccgcccgaaa attcggctct cggctctcgt ccctcgtcct
     9361 cggctctcga aaaccggcca taccattccc cctcttgggc acctccggtt catggcaacg
     9421 cgtccctcgt catggcgggt gattttttat cgatgaataa ctgatttgcg tgtaataata
     9481 tggggctgac accggattgt cagcccatcc actccatctt ccgctggtcg agcatgaaca
     9541 cccgcccgcg cggcgccctg cgcatccaag cctctcgctc cgaatcggtc gtcgacacgc
     9601 tggccgtcga agcccccctg caggtcaaca tcaacggccg gccctggacc accaccatgc
     9661 gcacccccgg cgatgacccg ttcctcgtcc gcggcatgct cttcagcgag ggactcctca
     9721 ccgaccccgc cgccgaactg ggatggcagc cgttcgatga tccggaatcg ggccacatcg
     9781 ccgccgtcga tctgacggtc gccgagtcgg cgctcaacca gccgttcgag gaccgccgcg
     9841 cgcagatgtc ggtcagttcc tgcggcctgt gcggcatccg ccagtggcgc gacctgttcc
     9901 ccggcgactt ctcaccccgc cttaacggcc agattcagct cgccgccgat ctcattcccg
     9961 ccgccatggc cgacctgcac cacggcatgc gcgcgttcgg cgagtcgggc ggctgccacg
    10021 gcgccgccgc ttacaccgcc cgcggccgcc gcctggctct gttcgaggac atcggccggc
    10081 acaacgccgt cgacaaggtc atcggttctt tgctctcccg cgaccagctt cacctggccg
    10141 tcctcctcgc cgtcagcggt cgcatctcct acgagatcgt cttcaaggcc ttccgcgccc
    10201 gcctgccggt cctcgccgcc gtctccgccc ccagctccat ggccgtcgag atggccgacg
    10261 cctttggcct gaccgtcctg ggtttctgtc gcgatacccg gctgaccgtt tactcccatc
    10321 ccgaacggat caaggcacag gcccaaccgc ggagtcgcac agcgcgccgg cgagccgccg
    10381 cccccgctcc cgccggccgg agggaccaac catgaaaccc attcgaaaag catgggaccc
    10441 gaaggtctgg gccagtctgc gtccgttcgg catcgggctg caacgcccca ataactactg
    10501 ggaactgatc cgcgcgctgc tcgaaaatcg cgaccggatc cccttcgcct ggcgcatcct
    10561 caccaagggg gtctgcgacg gctgcgccct gggcaccacc ggcatgagcg actggaccct
    10621 gcccggcacg catctgtgca acgtccgcct gcggctgctg cgcctgaaca cggctccggc
    10681 gttcaatccc cgcctgctgc gcgatctgaa ggggctggcc ggcaagcgca gcgccgaact
    10741 gcgcgacctc ggccggctgc cggcgccgat gcgccgccgc cgcggcgccc ccggctttga
    10801 acgcatctcc tgggaggaag ccctcgccac catcgccgcc aagatccgcg gcctggcctc
    10861 gcccgaccgc atcgggttct acctgaccag ccgcggcatg cccaacgagt cctactacgg
    10921 cgcgcagaag gccgcccgcg ccatcggcac caacaacatc gacaacgccg cccgcatctg
    10981 ccactccccc agcaccaccg cgctcaaacg ttcaaccggc gtcgccgcca ccacctgctc
    11041 ctacgccgac tggcttgtct ccgatctcat cgttttcatc ggctccaacg tcgccaacaa
    11101 ccagcccgtc gccaccaaat acatccacta cgcccgcaag aacggcgcgc gcgtcgccgt
    11161 cgtcaacagc tatcgcgagc ccgggatgga gcgctactgg atcccctcca tccccgaaag
    11221 ctcgctcttc ggcacgcgca tcagcgatcg cttcttcctg atcaaccagg gctccgacgt
    11281 cgccttcctc aacggcgcgc tcaagcatct gctcgccaac gactggatcg atcacgactt
    11341 catccgtgag cacacgcatg ggttcgacgc cctccgcgaa tcggtcgaac gcatgagctg
    11401 ggaggagatc gaggcgcagt ccggggtgcc ccgcttggaa atccaggcct tcggcaacat
    11461 gctcgccgaa gcccgccgcg gcgtcctggt ctggagcatg ggcgtcactc agcacgaaac
    11521 gggcgtcgcc aacgtccagg cgatcatcaa cctggggctc gcccgcggct ggctcggccg
    11581 cgagggctgc ggcctgatgc cgatccgcgg ccattcgggc gtccaggggg gcgccgagat
    11641 gggctgctac tccacggtct ttccgggcgg gcggtcgatc aacgccgaaa atgccgcctg
    11701 gctcgccgag caatggggct tccccgtccc cgaccggccc ggcctgaccg cccccgaaat
    11761 gatcgaggcc gcccaccgcg gcgagctcga tgtcctcttc tcctccggcg gcaatttcct
    11821 cgaggtcctc cccgaacccg actatgtcga ggaagccctc cggcgcattc cgctgcgcgt
    11881 ccacatggac atcgtcctca cctcgcagat gctgatcgac agcgccgagg aaacgatcct
    11941 gctgccggcg atgacgcgct acgaaatccc cggcggcgtc accgaaacct ccaccgaacg
    12001 ccgcgtcatc ttcagccccg aaattgacgg gccgcgcgtc ccccaggcgc gctcggaatg
    12061 ggacgtcctg ctcgagctgg cccggcgcgt ccgccccgag ctcgtcgacc gcctccgcgt
    12121 cgccggcacg cccggcctgc gcgaggaaat cgcccgcgtc atccccgcct acgacggcat
    12181 ccagcatctg cgccgccagg gtgaccagtt ccagtatggc ggcgagcacc tctgccccgg
    12241 cggcatctgc cccacgcccg atggccgcgc ccggttcttc cccgtcgaac tccccgatgc
    12301 gcgcttgccc gagggcgtgt tcgtcgtcgc ctcgcgccgc ggcaagcagt tcaactcgtt
    12361 gatccacgaa gagaaggaca tgatcaccgg cctggggcgc gaggccgtgc tgatcaatcc
    12421 ggccgacgcg cgcaagctcg gcctgcagga aggcgaacgc gttgaggtcc gcaacggcaa
    12481 gggcaaactc accggccgcg tccggctggc cccgatcaag ccgggcaatc tgcaggtcca
    12541 ctggcccgag ggcaacgtcc tgctcgacca ccaccggcgt tcctgcgaag ccggcgtccc
    12601 ggattactac gcgcgcgtga cgctgcaccg cctcaacggc caggccgcgc cgcgcccgca
    12661 acccgtcccg gccgagcgcg gcgtcccggc cccctgaggc tggcattcga ttttcaataa
    12721 aaaccgtcgg atcattccac tcgccgagtg cgttcccccg ccggctcacc cctgctccag
    12781 cacaaactgc accgccagcc ggatcatctc caggttgctg ctcaggttga gcttcttctt
    12841 aatgctctcg cgatgcgatt ccaccgtctt gctgctcagg tggagctgtt cggcgatctt
    12901 gcgcgtcgat aggccctggc cgatgagctg gaaaatctcc agttcgcggt tggacagatt
    12961 gtctaccggc gtttccccca ccacctcccg accggtcatc ccgatcagca gccgttcgga
    13021 catctcctcg ctcaggaaga tcttcccctt catgatgtgc cggatgccgt cgaccacctt
    13081 ctccgtcgag acctgcttgt tgacgtaacc catcgccccg gcgcgcagca cccgctcggc
    13141 gaacagcact tcgtcgtgca tcgaaaagac cagcatccgg atgttcttgt ccagcgcctt
    13201 gatctgcttg atcagttcaa tcccggtccc ttcctgcagc gacaggtcga tcacgacgca
    13261 gttgggcttc tgctcgcgca ccagccgcag cgcgtcggtc gagccgctcg cgtcgccgac
    13321 gacctccatg tcctcctccg cggcgatcag gtggcccagg ccatggcgca cgagcggatg
    13381 gtcgtccacg atcaccaccc ggatcgggcc gctctcggcc cctctggccc ccttaggctc
    13441 catcgaagtg tctgcctttc aggcgcagcg taatgaccgt gccggtcggc ttggccggtc
    13501 ggatttccag ttgcgctcca atctcgttgg cgcgatagcg catgatgttc aatcctccgc
    13561 ccagaatcaa atcctttgcc tcgggcatcc ccgtcccgtc gtcgctgatc cgcacgatca
    13621 gctcctcctg ttcgtggctg atctcgatct cgatgttgcg cggccgcgcg tgtttcactg
    13681 cgttgatcag cgactcctgg cagatcttga acaggttcgt cgcaatcccg ttgtcctcca
    13741 gccgcacggc cccgtcggtg tgcagcttgc accggatccc ggcccgcttt tcaatgatcc
    13801 ccagcgcgtc gcgcagcgcc ttcacaaact ccgccgcgtc gatctccacc gggagcaggc
    13861 ccttcgacat cccgcgcacc tggctgagcg actgcgccag accctcgacc aactcctcca
    13921 tcgtgtcggc ttcgggcaac ccccgcgctt gcagcttgcg tgccaacgtg cgcgcgatca
    13981 gccccagccc cgtcaactgc tgccccagcc cgtcgtgcag ctcctggccc aggcgccgct
    14041 gctcccggac cgacagctcc agaatctcct tctgcaaatg gcgtagttcc tcctccgcct
    14101 gcttgcgctc ggtgatgtcc ttgaccatcc ccagcgtgaa gcgcggctcg ccatgctggt
    14161 cgcggatcag cttcacgaac acgttgcccc agatgatccg gccgtccttg cgccggtagc
    14221 gcttctccac gctgaacgac gggatctcgc cctgcgcgag ctgccggtat ttccccaccc
    14281 ccgcctcccg atcgtccgga tacgtcagat ccagaaacgt cagccccagc agttcctcgt
    14341 gcgggtagcc gaacatccgt tcggccgccg cgttgacgcg gacgaagcgc ccgtcggtcg
    14401 tggccagcgt gatcgccagc ggcgattgct cgaagatcgc gcggaaccgc atctcgcttt
    14461 cccgcagccc gtcctccgcc atcttccagt cggtcacgtc ctgcacggcc gccaggatgc
    14521 tgcgcaggcg cccctcctcg gtccgcatga acgccacggc ccgccggcgc agccagcgcc
    14581 accccccgtc ggcgtgccgc acgcgaaact ccgtgtccac gcgctccccc tccagcatcc
    14641 gccgcagttc ctgccccgag aacgacaccc gcgtctggtc gtccggatgc acgacacttt
    14701 cgatcagccg gctgccctgc atcgcgaaaa accccggcga gtatcccagc atctgcgtga
    14761 tctgcgaatt cacatacacg ttgcgccgct cgatcaggtc gtagacgtaa accatgaacg
    14821 gcgccgtatc ggcgatgttc tcaatgaact ggcgactctc gcgcaactgc cgccgtgtcc
    14881 ggtcccgctc gtaggcgcgc cccagcacca gcccgatgtt gtccatcgtc tccagcagac
    14941 gctggccggg gcggaacggc gcgtcattgt aaaactcgat caccgccacc acatggcggc
    15001 cgatggcgat cgggaaaccg atcaccgcgg ggtaggggaa accctccggg atccggcgcg
    15061 agaagttctt ctcggcgcgg atatcgtcca tccacagcgg ctggcccgtt tcccggaccc
    15121 gcccgatcag cccctcgtcc agcgtgatca ggctttcggc cgtgatccgc cggtactcct
    15181 cgatcgccgg atcccgccga tttccatccg tataccagac ccgactcgag cgcagcagcg
    15241 gcgtgtcata atccagttga tacacatgcc ccagcggcca cttcagcgcc cggcccaccc
    15301 gatccagcgc cagccccagc agctcgacca gcgtctcgct ctcgttggcc gcgatcgcga
    15361 tctgatgctg caagcccagc agcatcgccc gccggtcgct gcgcgacatc agcgtctcgt
    15421 tgtcggccag caggtccccc tgccgccgct gcagcgcgct gacgtcgctg aaaatcagca
    15481 tcgccgccgg ctgatcggtc tccgttgatc cgaaccgccg cacctgccgg ctgtaccaac
    15541 ggtcttccac cggcccgacg atcgtctcga ccgccgccgg cgcccgatgg tccaggcccg
    15601 ccaacagccg gccgccgacg ggacggctga gcagatcgcc caacgggagc ccctgatccc
    15661 ccggacccag ccgcagcagt cggccggcgg ccggcgtgaa ggccagcagc cgccactcca
    15721 gatcgaacga cagcgccatc agatcgctgc ggttgacgaa ttcggtcaga tggcgatgcg
    15781 ccgtgcacag gttctccagc tcctgctgat agccggcatt ccggagctcc agttcctgga
    15841 tccgctgccg gagcgcgtcc cgttccccgg ttccgagctc ctcaggcggc gaaggctgtt
    15901 gcgacatatc tctgcccttt ggatgcctgc cggcgggaaa ccgcgcggtc ccgtgccctg
    15961 cgccgggtca acctcaccaa ctcacatcga tcgttctcgc caaggggaaa cattatacat
    16021 cctgactcgg accgatgaag cacgaaaacc gggaacgcca ctctcccgag gggccttgcc
    16081 ttcctccaga atcgtcctcg tcttcgctct caaaggccct cccagaattc ggctctcgtc
    16141 cctcgtcttc ggctcccgaa tcccatcttc accccagcac gccgcccctc tcctccgccc
    16201 ttcagcccct tcgtgtcttt cctcatcccc cactcttgat cccccgccat cgccgtttgc
    16261 attccgccgc cggatgcgct atcagttcac caccttcttt gtggaggctg atcccggcct
    16321 ccacgcactt gctcgctcgg tccatctctg ccggttcacg cctgtttccg agcccggtcc
    16381 gaaggtcgag ttggatggat acacctctcc cccgtcccga atatccgcgt cctcaattca
    16441 ttcgtcagca atggttgtgc ctgaatggcg cctggcagtt cgaaatcgac ccgggcgact
    16501 ccggcctcga gcgcggcctg ctcgagcggc ccctcgcccg ccagatcctc ctcccattct
    16561 gccccgagtc ccccctctcg ggcatcggcc acaccgatca tctgggcgcc gtctggtatc
    16621 gccgtgagct ccggattccc cccgaatggg ccgacgcccg cgtcctgctg catttccagg
    16681 cgattgacta cgacgccacg gtctgggtca acggccgcga gctccgccgc caccggggcg
    16741 gctttacccc catcacctgc gacctgaccg gcgtcgccgc accgggcgac cccgtcacga
    16801 tcgtcgtccg cgcccgcgac gaccaccgcg ccctcaagcc caagggcaag cagagccatc
    16861 tctatcgcaa catgggctgc cattacaccc gcaccaccgg catctggcag acggtctgga
    16921 tggaaccggt gcaccccggt tcggcgctcc tccgtccgcg catcaccccc gacgtggcca
    16981 acggctgctt cctgctggag cagccgatcg accggccggc cgccggcctg cgcctgcgcg
    17041 cgacgctcag cgacgaccag ggcgtgctgc tcacccgcga ggtgccggcc gatcacgatt
    17101 tcagcccgcg cctgatcctg ccggtccccc aaccgcgccg ccgtctctgg tcgcccgccg
    17161 atccgttcct ctatgacctg cgccttgagc tgctcgacgc ggccggcgcc gtcatcgacc
    17221 aggccgccag ctacgccggg ctgcgcggcg tgacgctcga cggcaacgcg atccggatca
    17281 atggcgacgt cgtcttccag cgcctggtcc tcgatcaggg ctactacccc gacgggatcc
    17341 tgaccgcccc ttccgatgcg gcgctcgccc gcgatatcga gctggcccag gccgccgggt
    17401 tcaacggcgc ccgcctccac cagaaggtct tcgaggaacg cttcctctat cacgccgacc
    17461 ggctcggcta tctggtgtgg ggcgaattcc ccgactgggg gtgccacgat ggccagcgcc
    17521 acgaggaaaa ccagcgcccc gacgccgcct acatcaccca atggctcgag gtcctcgccc
    17581 gcgactactc ccatcccgcg atcatcggct ggtgcccgct caacgaaacc gccgaaccga
    17641 tcatcgacaa gctgaccgcc ctcgacgatg tgacccgcgg catgttcctc gccgccaagg
    17701 ccatggacca gacccggccg gtcctggacg cctccggcta ttcccaccgc gtccccgagg
    17761 cggacgtcta cgacagccat gactacacgc aggacccggc ggagtttgcc gagcgccacg
    17821 cgcacaccgc cgcgggccgg ccgttcgtca acgagccggg cggccggccc gcttccatcc
    17881 cctggcgcgg ccagccctgt ttcgtcagcg aattcggcgg catctggtgg aatcccgacg
    17941 cgacacccgg cgagcactcc tggggctacg gcgacgcgcc ccgcacgatc ggggagttct
    18001 acgcccggtt ccgcggcctc tgcgaggccc tgctccgcaa cccggccctg ttcggctact
    18061 gctacacgca gttgaccgac gtttatcagg aacagaacgg gatctacacc ttcgaccgcc
    18121 gctgcaagtt cgatctggac cggatccgcg cgatccagca actaccggcc gccatcgaac
    18181 aggcccaccg cccgaccagg cctgacgatt cttcatcttg agtgcccgcg cccgcgggtt
    18241 tttgctggcc tcttcgccgt cttgggaagt tggtctcagg tctatgctcc ccgcaatcat
    18301 gcaattcaat agttcatcca ggtcttccgc cccacgccag tatcgttgtg ttcatcgcgc
    18361 tgaggatcag tcccgtttag aaactcctcc agattggtat agccatcgcc atccgcgtct
    18421 tccgtgctgt cgctgggatc agcggcgttt agctgatttt ccgtttccca atgatctggc
    18481 atcccatcca ggtctgaatc tgggggcgga gcgatggcgc cataaatcgg ccagccccct
    18541 gcctcgtcct gtgaatcaat aatctcaccc gtgccagcta cgacgttatt tatgatccgc
    18601 tcatcaatcg ggtcgcgccg cggaagcgtt gccccggcct ccgcgagaac cagatcgcgt
    18661 gcctcacccg gagcctgggt tatcgtgatc ggcatttcga ctggcatggt gagttgggta
    18721 taccgatcca caaaggcatt ccagccaata tccaacccat caagcacatt gtccacttga
    18781 tagattctgt aattatcgga ggtcggatga ttggcgccta ccccggactt gaaaatgata
    18841 tccacatcat ccgtcgtgga tggaccagtc tgaacccaat tccctatata attgatatct
    18901 gcccattcgc gtcggccgcc acctgcccca ttcacgtcgc cccagttgta aataatatta
    18961 tttcggaaat cgaggcgagt acggtgagtg ggatcatccg tgtcgctgcc aactcgtgga
    19021 ttgcgcgtat cgccgtgagc cagtagatta tgatgaatgg agacgtcccc gccgtcggtt
    19081 cgaatcagca ggtttgcccc gtggggacct tccgcatggg tcgagtcccg tagcggctct
    19141 gaaataatat tccattggaa agtgatgtgg gtcgtatctt catcgtccgg accgcccgca
    19201 tagatctgca cacactcgtc caccgcccag gagaacgaac aatgatcgac gatgacattt
    19261 cgtgctccct ccagcgcgag tgcatcgccg ggccagtcct gcggcaccgc gcccccaatg
    19321 tcgcctggac gaaagcgcag atgacgcagt atgacatcgt gcgtccggat cgtgatttgg
    19381 ttcccgcgaa agcatatacc gtcgccgggc gccgtttggc cggctacggt caggtagggt
    19441 ttgtcgatcg tgaggctgct gctcaggtcg ataatgccgg agacatggaa gataacggtg
    19501 cgcgggccgt gggtttcaac ggcttcacgg aagctaccgg gacccgcgtt gttcaaattt
    19561 gtaacaaata atacctgccc tccccgtcct ccccgggtgt cagcccctgc cccttccgca
    19621 ccgggaaagg ccttctgggc ggccgcggac ttgacgtgca gggcgagcag aacgagtccc
    19681 catacacgga gcagccatag ccagtccggt ttcagcaaag caaagtcaag ccccgcgcgc
    19741 attgatgcca tacccctaaa cccggatgat ctttcgcgtt aagtagccca gcgggcagcc
    19801 tgttggggta gtcccgaaag aatggaacat ttcagtccgc cactgcaggg tacggatgcg
    19861 gcgggttaaa atgccggcgg ttttctgctt ctatctggtt tccgcccgcc gcgcgcgctt
    19921 ccgggcccat tcgtgtcgtt cgtgatccag aattcttttc tcttaatgat gcggggcgcg
    19981 ggcgcagtgc tcgcaattga cccagcccga gtccccttcc ggatagaatt ccttcttcca
    20041 gatcggcagg cggtgcttca cttcgtcgat gatgaactgg cacgccgcaa aggccggccc
    20101 gcgatgtacc gcgctgacgc cgacccacac cgccaggtcg ccgatcccga gcgcgccgac
    20161 gcgatggacg caccgcgcgt cgagaatctc aaaccgttcg cgcgccgcct cgaggatccg
    20221 ctccccctcg cgttcggcca gcgcgccgta ggcctcatac tccagccgca ccacctcgcg
    20281 cccggcgtta tgatcgcgga cccacccctc gaacgcgaca aagccgcccg cgcgcgggtc
    20341 ggcgagcgcc gcccgcagcc cgtcgcgctc caggggcgcc ggactcagcc gaaaccggcg
    20401 ttccccgccc gccccgcggc ccgcgttctg atccgaatac tcgccatcgc cggaccgcat
    20461 gccgcctcac ccccccgcca cgggcgggat gaagacgatc cggtcgccct cggccagcgg
    20521 cgcgtcccac gcgctgaact cgtcgttgat cgcgacgcgc agatgctccg gccccagtgc
    20581 cagcgcgtgc cgggccgcca ggtcgcggta aagctcgccc gccgtcgcgg ccccggtggc
    20641 gagctgttcc cgcgccaggc cgcgctgctc gcgtaggatc gcgaaatact gcacttccac
    20701 gttcaggctc tgcgtcgccg gcatgatcct caccctccga tcgccgtcat gctgcggttc
    20761 cgctccgcat cgatcgcgcc catcggctcg tggccatacg gcttgagcga cacggcccgg
    20821 cgaatccgcc gttccagttc ctcgtcgccc gcccccgccc gcagcgcatc gcgcaggctc
    20881 gcctcggccg ggtgcagcag gcacgacttg atgctgccgt cggcggtcag ccgcaggcgg
    20941 ctgcacgccc cgcagaagtc gcgcgtcatc gacgtgatga accccaccga acccgccagg
    21001 cggcggcccc gtcgatccgt cacggtgaac tcgcgcgcga cgttgctcgc cccgtcctcc
    21061 cccgccgccg gttccagccg atagtcgcgc tccagcgccg tgcgcatttc gccatagctg
    21121 accagcttgt cgcccgacca cgcattgcca tggaacggca tgaactcaat caagcgcatc
    21181 tggatcggcc gcgtccgcgc gaattccaca aaatcggtca gttcgtcgtc attgatgccg
    21241 cgcatcacga cgcagttgag cttgagtggg gcgaatccgg ccgccagcgc ggcttcgatc
    21301 ccgcccatca cctgctcgaa attccgccgc agcgcgatcc gctcgaaccg ctccggccgc
    21361 aatgtgtcca ggctgatgtt caatcggctg agccccgccg cgcgcagctc ggccgccttc
    21421 tcggccagca gcacgccgtt ggtcgacatc gccaccgtcc cgatgccggc gatcgcggcc
    21481 agctcccgca cgagcccttc caccccccgc cgcaccagcg gctcgccccc cgtcagccgc
    21541 accttgtcga tcccgagccg ggcgaaaacc cgcaccagcc ggatgatttc ctcgaacgtg
    21601 agcagatcct cgcgcggctt gagcgggatc ccggccgccg gcatgcagta caggcagcgc
    21661 agattgcagc gttccgtgat cgatacgcgc aggtaggtgt ggcgccggcc gaacgtgtcg
    21721 atcagcggcg gtcggacggg ttgcagcgcg gcgggagtca ggggtcgatc ggcctgcacg
    21781 gaaaaaaaga cctcgacatt gattttacgg accccatccc gcccctgtca agccgctgca
    21841 cccccctgca cgattcgtcc ctctcccctc gtcccccctc ccttgtcctc gcccccatcg
    21901 gcccaaccca tagttcggct tccggctctc gtccctcgtc ctcggctcgc gaaaaccgga
    21961 cagattaacc ctctccgtgc aagtcgcaat ccaccggtgt gggaccggcg cccgcgccgg
    22021 tcatgcccct ggtaacctca cgaatcaccg acgcacgcca acccgccagc ccgcgcccga
    22081 gcgcttttcc ctgcccccct cgggggccct gtcattcgca ggggcctcgg cccggtgact
    22141 gatcgcgata aatgtttccc aaccgttttc acgctttccc tcgcaaccca tccaatgtca
    22201 ccccccgcct ccctcttcat ttcgagtccc ttcatgcctt tcgtgatcag cgtgccccgg
    22261 cgcttcttca ttgcgtcgcc gaacgcgacc ggagataatc gacatccaac tttggttttc
    22321 tcacccccgg cggccgcttc ccgcccccag gctcggccgc cgcgcggaaa ggtgcaattc
    22381 atgatgagct ctgttcgctt ccgtctcacc tgtgccctct tgcttctggc caccgtggct
    22441 tccgccgaga cccgcaaaat ggtggccttt cccgccgcca cattcacctt cggcgccccc
    22501 gtcgtcaaca ccaacggcgg ggtgttcgtc gcgacctccg atgtcaacat ctacttccag
    22561 gatggcagct cggccgagcc caaccggatc ttcggcgcgg gcgattccgt cgccggcggc
    22621 accttcctca gcctcaacaa tttccgcccc atctcgccca tcttcggcgg cgatggcgtc
    22681 ctcgccctcg gctcctatga attggaggac tcgggctccc aacagatcct gatcgccctg
    22741 gatgccgatg gccatctgct cggccaggtc ctcgcctccg atgccgcctt ctccgagatc
    22801 aacaatccgg tcatcggcct gcccgcggcg gtccatctgc accgcaccgg caaggccggc
    22861 gtcaacgacc tgggcatttt cggcgcggtc ggaccacaac ccgccccgcc cgatgagccg
    22921 tttcaactct tccatgtcgg ctttatcgaa ggcaacttca cccccatgct cgtggacacc
    22981 ccgtatttcc ccccgcaatc gggtgccaac ggcttcaaca attcattctt ccgctaccag
    23041 gggccgctgc tgggcgggac caacggcgcc agcgacctct tccaactgac cggaccgatc
    23101 gatcccagta tcgatctgcc cgctcccgag ggcctgaatc cagacgatgt ggccgctttc
    23161 ggcttgctca tcaggccgga ggtcgaaggc gtggccgcct ggtcgcgcga gcttctgcgc
    23221 cgcgtcaatg atgcggacga tccgggcacg ccgcccgatg cggaaccgat cttcacgatc
    23281 accttcaatt tcgaggcttt caacttgact cgccacaccg tcattttcgc cggcaatgtg
    23341 cagcagaccg cccccgagat gggcggcgag gagcaggccg gcggcctcgc cagcgacacg
    23401 ctcgatacca ccgacgaaaa ctcggactgc gattgctccc aggccgatct cgatggcgac
    23461 ggcgacgtcg accgcaagga cgtccgcatc gcccgcaagt gctacctgga ggggggcggc
    23521 gaccaatgca aggacgtcaa cggcaacggc gtcgccagcg agttacgcga ctgggagtgc
    23581 gtccagtact gcgccaccca ggagggcggc ggcaacaagg acctgatcaa ccccggcagc
    23641 gcggtgttgc tgctgcgcca gggccagccg ctctacttcg agccgtcgct cacgctccag
    23701 ggcttcaacg ccgtccgcgg caacggcatc ggcgccggcg cgttcctcgt ttcggtttcc
    23761 ggcccgcagg gcttctcccg cgcgcttgtc gggatcgatg ccgacgcctc cgcccccaag
    23821 gtcctggtca agaccggcga tgatctgggc gacggcccca tcaccgacat ccagaatctc
    23881 agcaatatca acgaactggg acaggttgcc ttcacggcga gcgtctccgg cgcccccggc
    23941 ccgatcgtct accggaccga tatcgtcgcc ctgcccgaaa acgccgccga ccgctgggaa
    24001 gtctatttct gatcagccgt cagcgaagcg aaacagcggc acgcaaccgg cgcattcctg
    24061 cttcaaaatc cgtcggatca caccgatccg acggatttcc gcctcccccg tccacccttc
    24121 aatctcaaat cgtcccttgt cctcgtcgtc gcgatcttcc ccaacggccc acccaaaatt
    24181 ccgctcccgg ctctcgttgc tcgtcctcgg ctctcgaata cccgaccacg gggcagtccc
    24241 cgcccccccc gtccactctg tccgcttcgt cctgcctctc cccgtcgccc cgcgccctca
    24301 ttgcttgcgc gaaaccagca tctcaatcac cgcccccagc ggctcggcca gttcgcgcgg
    24361 cagacgctcc agatgccgcc gcgcgtcttc gtgcagccgc cggcagtagt cccgccccgc
    24421 cgccacggcg cccagcgcct cgaagatcgc gaacgcctcg tcgatgtccg cccgcgtcgt
    24481 cgcctcccgc ggtttgtcca ggatatcgaa cagccgttcg cgctgcgccg gcgccccgtg
    24541 ctgcgccgcg tacgcgacca gatacgaccg cttcccttcg cggatgtccg atccgaccga
    24601 ttccctccct ttgccgtcgg tcaggtcgat gatgtcgtcg atgatctgaa acagcggccc
    24661 gagatactgc gccatctcgc cgatcgtctc gatcacccgc tggctcgccc cggcgacgat
    24721 cgcgcccccc tggatcggcg ccgccaggta gtagcccgtc ttttcccgca cgatccgcag
    24781 gtattcctcc tcgctcaggc tgcgccggtg gcggtagttg atgtcgagcg cctggccgac
    24841 atgcgtatga tcgagcgccg acgtcaacag ccgcagcagc cggacgcgcg tcgcgtcgtc
    24901 cagcccgtcc ggctccccct cggccagcgc cgccatcatc tgcaccagca ggtaatcgcc
    24961 caggttgatc ccgtgcgccg cgccgtagcg cacccaggcg gcgtcgcggc cgcgacgcat
    25021 cacgtcgccg tcctccatgt cgtcgtgcac cagcgcgaaa ttgtgcatca actcgatcgc
    25081 cagcgcgaac ggcagcgccc gccgcggatc cgctcccagc gcctccgccg tcagcaggca
    25141 cagcagcggc cggacccgct tcccccggcc cggtccggcc ggcaggtcca gccccaacgc
    25201 atagcgcaat ccctcatgca ggttctcgat cggttgcgcg gatcgtgcgg cgacgaaccg
    25261 ctccagggct tcctcgaaca gggcgcccag cgcctccagg cgcccctcga gcccggcacc
    25321 cccttgctcc gcggcgggcg gcgtcgactg cgtcattgca tcagctcttc ccactggatg
    25381 atgcctgcca gggtcaggcc cagcaggaga accgccagcg cgacgcgata ccagccgaac
    25441 gggctcagtc cgtgccgcgt cagataggcg acgaatccct tgacggcggc ccacgccacc
    25501 accagcgacg tcagaaaccc gagcgccaga ccggccggcc cggccgcctc caggatggcg
    25561 cccccctcgc ccagcagatc atggcacgtg gccgcgccga gcgtcggcag cgcgagcaga
    25621 aaactgaatt ccgccgccgc gcgcggtgaa tatcccagca gcaacgccgc cacgatcgtc
    25681 accatcgagc ggctcgtccc cggccacatc gccaggcact gcgccacgcc gatcagcaac
    25741 gccgcgcgca gcgtcatcgc ctccatcggc aggccggcct ggcgcgcggc cgccgcgccg
    25801 ccggcccggc gcgaccgcac ccgctccacc gcgatcatca gcgccccccc caccgccagc
    25861 gccgccacca ccgggcccgg gctgaacagt ttcgccttga tgaagtcgtt ggccacaacc
    25921 cccaccaccg cggctggcag aaacgccgtc acaagctgcc ccagcagcgc ccgtcccccg
    25981 ccgtcccggc cgagcagccc ggccagcatc gcccgcaggc tcggcaggta aatcgccgcg
    26041 accgccgcca ccgcccccgc ttggatcacc acctcgaacg ccttggcgcc gtcgctggcc
    26101 gggatcccca gcaacgccga gacaatgatc agatgcccgg tcgacgagac gggcaggtat
    26161 tccgtcaacc cttccacgac gcccagcagc gcggcctgtg tcagcgtgat gtcgtaagcc
    26221 atgggcgggg tggaggggtc ggcaccgggg gatcacgtcg cggcgacgcc gcaagccgac
    26281 ccactatgcg gcagaaacac cacgcgcgca accttccgat ggctccgatt cccccgcccc
    26341 ccacaccgac gaatcgctat cggtatcggt atcgccatcg tcttcgcttt ggtcctcgcg
    26401 caagaatcgt cctcgtcctc gtgatcgccc cagcggcccg ccccgatttt tctccttcgt
    26461 tgcggaccat cggttcatga acactcgtca tcggtggttc acaatgaggg atgaaaatgg
    26521 ggagggaacg accggttcga acgcgccagg acttgccgga tcgttcaaca gagccgtccc
    26581 gccagagacg gcagacttcg gcgggaacca ggtcacccat accgacggcg tcccgcaggg
    26641 acggcagaac cgctccaggc gatcccggga atcggagaca aattcttgag tgcaaacgaa
    26701 cggcgagcaa acgacagacg agagcagagg accggcagcc gacctcaata gctgacgtaa
    26761 atcacgtcgc catcgctgat cgtgccgttg gtggggtcgt aactgtagcg ctcaacgagt
    26821 tcgcgcagct cgcgcgcggt cagcggtccc cccatcggga accccgccga ctcggccgag
    26881 accagcgtgg gatgatcctc gctgcggatc tggcggatgg cctctttcag atcgctgtcg
    26941 ccgtcggggc cgacgctcac cagcacccac gcggtcgccg ggccgatatc cttgtaaaac
    27001 tcgaatttgc gctcctcggc gccgtcgggc acgaacgggt cgtcgggaac ataatggccg
    27061 cccccggccg gctggctcag ctcgcggacc cacgtggtct cgcccggcag ccggccggtg
    27121 taggcgcgaa acgcctcgaa ctcactggag aaattgcgct gggtctgaat cgtcgccgac
    27181 aggcgcgtct tgaccatcgc atgtttcgag gaaaatacga agatgacggc gagcacagaa
    27241 aatacggcca gaacgatcag catctcaccg taggtgaggc cgcgaaccga ccacgcgcga
    27301 tgcaaggggc gatgccgcat gacgatccgt ccgtctgagg ggggacgacc ctcccgcaca
    27361 acaccgatta tgctgaggac cggatgaacc cgccacaaaa aaagtggcaa gaaacgcgcc
    27421 catccggcca aacggcccgg gttacttgcg aatacttccg ccgtgaagga tagcccatca
    27481 ccggcgtcgc gtcaagtcgc caactctctc gacacgcgcc cccacccgtc tgttccacca
    27541 cccccacaaa aatccgtccc tcgtctctcg tcctcgcctc caacggccta accccagact
    27601 cggctcccgg ctctcggccc ctcgtcctcg gctctcgaaa tcccctgttc aattcctcgc
    27661 ccttccccac ggctcagcga ttctgaggct taatcacgat tgtcccagag gaccaacgca
    27721 ggcgtatttg ccttatttgc ccaagtcaat ccgccgagcg gacgtccgga ttcaagcgca
    27781 gcgtggaggc cgcagcccgg aaccctgcgt cgccgccccc caaagcccca aactcctgca
    27841 aggggaggcg ggagattctt acgcgtcatt gatgtccctc ccaccaatcc agcgaaagct
    27901 cattccggcg gcagcggctc gtcccctttc ttgccccccg cgatccaggc cttgcgcgcc
    27961 tgcaggtagg cgcgtttctt atccagttcg ctgatcggcg cggccggcag cgcactcgcg
    28021 cccggcttct ccccggcgcc cccatcgccc tcctcccccc cggcggggct cgccggcagg
    28081 tcggtcgcca gcgccgcccc ccgcagccga tgcgccagtt ccgcgtcttc gatcaggatc
    28141 tcgatctgcc gctcataaag ccggttgtag ggcggacgct tcggcagcgt ccggcggatg
    28201 aaatccagca gccgctccag tcgcgccccc ttcaactgcc gcggttgacc cagcaacgcc
    28261 tcgatcgtcc gcgtaaactc cgcttcctcg ggcagcggct gaaaaaactg cgccagcgcc
    28321 gtctgcgccg cgttggcgat caccgggtcc ttgtcgccga acagctccag cagcacttcg
    28381 gccgcctcgg gccgccgcgt ccggcccagc gtcgccagcg cctggatctg cgtcggcttc
    28441 ttgaccagca tccccccctt cgcctgcgac agcgcatgct tcagttcctt ctcgtccagc
    28501 tcgagcacct cggcgcggcg cgacacccgc cccagcccgc cgtgtttcat cttggtgtcc
    28561 atcagcagcg cggcggtctg ctgacccggg atgcggatct gccccgcctg catctgatcg
    28621 gccaggtcga gcgccgcctg gatccccgcc tccccccccg attcgagcag cgtgtagatc
    28681 agcgtgtgga tcaggtcgtc gcgattgagc tcgaccagtt cgccgctggc ggcgtcgagc
    28741 accttctcct ccagcacctg cgaccgcggg cccgcttcgt catgaaacag cgcggtgcgg
    28801 atcatcgcct ccagcagcgg gaccgagcgc ttgtcgccga tcgtctgcag cgcattggcc
    28861 agcgccgcca tcgccgcgct gtagtgcgcc gcatgcttgc gaccggcctc gaggatctcg
    28921 tccagcgcgt tgcggcccag ccggcgcagc gtctcgacca tcggctcgcg ccagccattg
    28981 accgagatgc gcgtcccctg aacgtccagc ggcggtcccc acatcgcgtc gacggcgtgt
    29041 tgcgcggccc agcgcagcag ttgcggatcc agttcctgca gccgcaccga gaacatctcg
    29101 gccgtcgctt tgaacaccag cgcccggtcg ccccgctcaa ccgtcgccag caccttctgg
    29161 atcacctggt tgcgccggtc gggatcgatc ccgcgcaggc gggcgacgcg acccagcgcc
    29221 gtgatcagca gttcggcgcg ccgcgccgcc cccttctcgc ccagccgctt gaccaccttc
    29281 tccagccgct ccagcaggat ctccgccgcc cgcggttcat tgatccggcc cagcgcgtcg
    29341 atgaaccagc tctcctccaa gtccgaactc gtcttctcga atccccgctt gagccagggg
    29401 aagaagtcgg cgtgccgcat cccgcccatg atgcccgtca gcagcttggc gcgtccggcg
    29461 gcgtcggccg tatcggcgaa tttcaaaatc gccccccgca gattgtccag ttcccccagt
    29521 tgcaccatcg tccgcaccgc cgtcagtttg gcgcgcggcc ccagttgcag gaacatccgg
    29581 tcgcccagcc cggcgtgtcc ctcgaacggc gacggatcct tggggcaata ctccacgacg
    29641 atcgacagcg catcgatgaa gtcgtccgga tcgcgctgat gcgcgccggc cttcaggatc
    29701 cgcagcagct cgtcgcgaaa ccgggcgatt tgttcctcgg tcggctcgcc cgcctcgttc
    29761 gggcccggca ggcagtcgcg cagcgacagc accgcctcgg tcagttgctg gtaccacgcc
    29821 tggctcgtcg cttcaaactg gcgcggcccg ccctgcgcga cgaattcctc gaacgaggcg
    29881 gcgagggcag cggcgccctg accggcctga ccccccgccc ggcccagtgc ggcgatcacc
    29941 ttgcgcgagc ggtcgacgtc gcggtggagc tcgcccagcg cccggccgag aaaccgcgcg
    30001 tggtcctcca gttcgcgctc gccccgcgcg cgctgcagca gcgccgccgc ccgctgcgcc
    30061 gccgtcttca gatcctgcgt cacgatccgc tcgcggcatt cgcgcgccag ccgctcgcgc
    30121 gtcacccccc gcaggatctc cagccactct tccgccgtgc gatgttccgc catgaacttc
    30181 actccctgcg ccgtgccgcc gattgggaac cccgatctta gccgattcgc acggataatg
    30241 ccgaaggaag attgcccccc atcccatcct caccctgctc gtctccggct ctcgaaaacc
    30301 ggccgttttg tcccttcgtt gtgcagcccc cggccgtgta gaatcgcacc atgcccgccc
    30361 cgccgcccca tggcctgatc tacctcgatc actccgcgac gacgcccatc gaccccgaaa
    30421 tcgccgcccg gatgcgcgac gcggatgccc ggttccccgg caacccctcc agcatccacg
    30481 ccgccgggcg cgccgcccgc ctctgcctgg aggaagcccg ttcggcgctg gccggctccc
    30541 tcggcatgcc ggccgaatcg gtgatcttca ccggctcggg ctccgaggcg aacaacctgg
    30601 cgatcgccgg cacgctgctc gagcacgccg gccgccccgc gcatctcatc gtctcggcca
    30661 tcgagcatca cagcgtcctc cactgcgccc gcgcgctcgc ggcgcgccac gacggggtca
    30721 ccctgaccga aatcgcctgc ggcgccgacg gacgcatcga tctggacgca attgccgacg
    30781 ccatccgccc cgcaacccgc ctgatcagcc tgatgctcgt gaataacgag accggcgtga
    30841 tccagccggt ggccgaggcg gcgcatcggg cgcgcgaccg cggcatccgc ctgcacgtcg
    30901 acgccgtcca ggccctcggc cgcctcccgc tcgatttgcc cgcgctgggg gccgacctgt
    30961 tgacgctggc ggcccacaag gcgcgcgggc cgcgcggggt gggcgccctg ctggtccgcc
    31021 ccggatgcgg cctcgcgccg ctgatccacg gcggcacgca ggagaactac cgccgggcgg
    31081 gcaccgagaa cctggccggc gcggtcggga tggccgaggc cgtgcgccgc gccgtcgccg
    31141 cgaccgacgc gcatcgccgg cacctggcgc gcctcgaacg gatcttcctc gacacgctga
    31201 ccggccaggc cgccgccgga ccgcgattcg aattaaacgg ctgcgcgact gcgaaggtcc
    31261 ccggcatcct caacctcgcc ttcgaaggcg tccgccagga tgatctggtc gtcggcatgg
    31321 atctggccgg cgtcgcgatt tcggccggct cggcctgctc ctccggcgtg atcgaaccct
    31381 cgcacgtcct ggcggccatg aacctccccc cctggcgccg cgatggcggc gtgcggattt
    31441 ccttcggttc caccaacacc gagcaggaag cgcggcaagc cgccgcccgg ctcgcggagc
    31501 tggcccgccg gctcgcgact tccaccgccc atggctgacg atcccccccc gctgacggcc
    31561 cccgcgcggc gaatcccttt gcattcgccc cgccggcctg ctaacccaat cgcatggatt
    31621 cggcggcgtc actcagagaa ggatcctcat ggcccgttat ctcctctgct tccatcacca
    31681 gaccgacccc gcgcaggcgt tgccgtggca ggagccgctc gagcagttcc cctgttcctt
    31741 gctgcgcacc aaaacgctgc acgaagccaa gacctactgc cgtgcctacc cgctggtcat
    31801 ggcgctcgtc cggttccatg agcggacgcc cgagctcgtc gacgccgtcc gcgatctggc
    31861 ggcggccatc cccgagggca aacgcctggc gctgatcggc ttgtccgatc agccgctggc
    31921 ggccgccgat cgggccgaac tctgcgccgc ggggctctgc gatttcctgg ccgccgagga
    31981 tccccccgcc atcgtcctct ggcgcctgga gatcctctcg ttgctggccg acctggacca
    32041 gttcgccaag gcgcgcatgg acgtcggcga actggcgaaa aaaacacgcg agcggctcca
    32101 cgacatgagc cagcccctca ccgccgtcca gggacgcctc cagttgctcg ccgccaaggc
    32161 cacccccgag gaccccaacg cggagtatta ccgcgagatg gtccgcctga tggccgccgc
    32221 gacgcgccag atcgccgaga tgcaacagct ccaccgcgct ttttcctgat cccccagaga
    32281 gccagtgagc tgctgtgaac aaccgcttcg aacgactgcg accttgccgg actcggttca
    32341 cggccagtcc cgtctgggac ggcagaaccc gcaacgcaat tcgtccctca cttaacaatc
    32401 gtccctcgtc cgcgtgatcg tcctcaaagc cccgccccag attcggctct cggctctcgt
    32461 ctctcgtccg cggctctcga atactgaccg catctttcct tctccccccc tcgctcctcc
    32521 cgccgccctt cctctcgccg ccctttccct cccatggacg ctgcgtcagg gccgtgctac
    32581 gattctcagc acatacccgg ctctcccccg gctctccggc tgaaaaacat tcgaaccgaa
    32641 accatccggc tgacgactcc gagacgaatc cgaacggaaa gcgagatcgt ggcatgtccg
    32701 cccaggcttc cgagccgacc cccgccccgc cgtcgcgggc tcaggccgcg ccccgccccc
    32761 cgcgccgccg ctggccccgc atcctcgcgc tgctcgcgct gatcctcgtc atcctggcgc
    32821 tcgcgctggt gctttacgcc caatcctcgc acgcgctgcg ccatgtctat ctgccgctga
    32881 tcaacaagaa tctgaacggc gaattgagcg cgcaaggggg ttccgcgtcg ttgcgcggcc
    32941 ggctccatct gcgccagccc gtcatccgtg acctgggcgg gaacgcgatc ttcgaggccc
    33001 gcgaactggt ggtcaacgtc gcgccgctct cgctgctgcg gcgcaacgaa gccccccgcg
    33061 tcaacgagct gcggctcgcc tccccgcgca tcgtcgtcca ggtcgaaccc gacggccacc
    33121 tcaactggga gaaactgacc aagggcgccg aaccggtcgt cgaaccccag cacgaacccg
    33181 acgacgagcc ggacagactc ccggcggtcg agatccgcaa gatcgaaatc agcgacctgg
    33241 atctgaccta ccgcgaccgc gacggcctgg agggccggct ggaaaagacc agcctcaata
    33301 ttgccaactt ggcgccgggc gaaaccgccg ccatcaatct cgatctgaac ggctcggtcc
    33361 gccagcccga cgccggcatc gaccagcgga cccgccttac gctcaacggc gaggtcaccc
    33421 aggacgacga ccgcgacggc ctcggctggg agctcaacgg cgaactccgc agcgaaggcg
    33481 cttcccccgc cgcgcccgag cccgtcaccg tccagatgac cgccgccacc gccggcaact
    33541 actcgctgac gggcgactgg accatgcgct tcgagatcgc ggccgacacg ccggaaggct
    33601 ccgtcggccg gttcaccggc gatgcgcaat gggcgcgcac ggccggctcg cgccaggcac
    33661 acgtcgaatt cagccagatc agccgcgaat tcctcaaccc cctgctcgcc gccgccggca
    33721 tgcagttgga acaggccgcc ctcgacgggc gcatcgacgt ggagggttcc gccgacgccc
    33781 tggcgttcga ttcgacgatc aacgccgcca acctcagcct gcgcgtcggc gccggcaagc
    33841 cgaccccgcg cgtcgacgtc gccttccgcc aggccggctc gctcggcttc gagccgcgcc
    33901 aggtcaactg gcgcgaaatg ctgctgactg tcgatgaggg cgggcggcgc gtcgtcgaag
    33961 tcacgctcga tcagcccctc gtcctcgacc tggcgcgagc gcaggccgat gccggcggcg
    34021 ccctggccgg cgagaccgcg caggtccagc tcaacctcaa cgatttcagc ctcgacgagc
    34081 tgggaccctg gctcgccgcc ctgggctcgg ccccctccga ccaggtcccc agcggcacga
    34141 tcaacgccga tctgaccctc cgggtggcgc agcttggcgg ccggatcgac gtgaccggcg
    34201 ctgtccgcgg cggcgccctg cgcggcggtc cgctcggcga cttgccgttc gacctgaacg
    34261 ccgatctcgc ggccgaactg accaaccagg aacaactggc catccgccag gccgacatca
    34321 acttgcgcca ggaggacgcc gagctggctc acgcggccgt cagcggccag ctcaacctga
    34381 ccgcgcagga cggccagctt gccctggtgg ccgagctgcc gcggttcgcc cgcgcgatgc
    34441 gctcactcgg ctatctcccc gcccagtccc cgctgagcga atcgggtccg ctgcgcgccg
    34501 acgtcgacct ggcgctgctc ggcccgggcg ccttccgcct gaccggcgac gtccagggcg
    34561 acaacctgca ggtgaccaac ctcggcggcg gaccgctgaa cctggcggcc cgcctcaacg
    34621 ccgagcgcga cgcgaagggc ctcatccagt ttgagcagac gcgcctggtc gtcagcgagg
    34681 gcgccgccgc gctggcgacc atgacgctcg ccggcggcat caacccgacc gagcaggccg
    34741 gccaggtctc gttccaggcc gacctgccgc aggcggtccg cgccgcgcgg atgctcggcg
    34801 tcctgcccgc cgattccccg ctcgccggct ccggtccggt cacggccgac ctgaagatcg
    34861 ccttcagcgg caagcagcgc ctgcaagtcg agggaatggt cgcggccgac gacctgctac
    34921 tggcggctgg gagcggcggc ccgctggacc tgaacgcccg gctcaaaggc acgcgcgacc
    34981 ccgaaggcct gctccagttc gaggatacgc gcctgacgct cagcgagggc gaggcgcaac
    35041 tggccagcgt gcgcgttacc ggcggcgtca atcccgccgc gttcaccggt caggtcgaga
    35101 tcgaggccga ggcgccccgc ctgctcaccg cgctccagcg ggcgggcctc atcgacccct
    35161 ccgccaccga cggcgtcggc gacggcaagc tgaccgccgc ccagagcatc aacctgcgtc
    35221 aggacggcac gattcacgtg acgggccgcg ccgccgccga aggcatgtcc gtctcgctgc
    35281 gcggcagccc gccgctggaa ctcggcgcgc gcctccagaa cgacgcctcg ctcaacccgc
    35341 gcacccgcga actctccctc gctccgctcc ggctggagct gcaacagccc ggcggccagc
    35401 ccggcgtcat cgaggcccgc ggcgactgga gcctcgccgc ggcgggcggc ggcgaacgcc
    35461 gcatggaatt gacgatcacc gacgtcgacc tggccccctg gctgcgcctg acccgtccga
    35521 tgcgcgtcga tgacctgccc cccatcccgc tcaacgcccg tgaaacgctg atcgccgatg
    35581 cgcaaggcgc cgtccagatc ctgggcgagc tccacatcgg actcccatcc tctgcggaga
    35641 gtgccgcact tgacgtcacc gtgcgcaacg aactggctca ggtcggcgac cagatccagc
    35701 ggctgattgt ggagctgacg tcgcgggcca acggccagcc cgccgattcg gcgcgcatcg
    35761 agggcgccgg cacgctcggc gacgcccccg atctccaatt gaccgccaac atcgaacggt
    35821 tcaacgccaa cccctatctc gcgctggccg accgcctcag cggcgacgcg gaggcgccct
    35881 ccggcgggac cgcttcggca aagacgaccg gcgccggcga ggcgtcgagc accgccgccg
    35941 ctccgcccaa cctgacgctc cacgccgatt tcacgatcgc tcgcgccgag gcccgcgagg
    36001 ccgtcctgac cgacgccagc ggacgcgtcg aaatggccga cggcgtcgcc cgcattttcc
    36061 tcgacgaagg ccaggtcaac ggcggcccga tccgcggcga cgccctcctc gccatggccg
    36121 gtcccgaacc gcgttacgag tggaacatgg ccgtcgagga ggcccgcgtg gagagcctgg
    36181 tcgattcctt cgccccccgg ctgaagggcc ggctcaccgg aacgcttgac gccagcagtg
    36241 ccggctcggc ccgcgggtca ggggaggcgc tgaaacgcca gctccagggc gaggcgacgt
    36301 tttcgatcgc cgacggccag atcgccggcc tcgaactcct cgagctgctc gcccgcgaaa
    36361 cccgcattcc cagtttcgag tcgttcaaat tcttcagttt cgagggcgac gtgcacctgc
    36421 gccagggccg cgccgaactc gatggcgtcc gcatcgtcgg cccgagccac gccctcgcca
    36481 tcgagggctt ctacggcctc gatggccagt tggacctctc catccagccg gccgtcgccg
    36541 ccgacctgat cccccttgaa acgaagaacg agtacctgca ggccctgctc gcgccgggcg
    36601 aaaccggact gacccccctg cccctcaagt tcggcatcgt cggctcgacc ggcaactaca
    36661 gcgtccggcc ggaggtcgcc ctcccggcca gcagggaaca gctgggcaac acgctgcgcg
    36721 gcatcgtcgg cggtgcgctc caggaccgcc tcgccggccg cggcgacccc gacgacggcg
    36781 aggccggcgg ggagaagcgc gaatccctcc tgcccgagtt gccggagctc cccggcctcg
    36841 gcggcctgac cggccggcgg gatgctcgcc gcgcgattcg cgccggggat ccgtccgagc
    36901 ccgcccccgc cgacgagacg accgggccgg cgcccgcgtc cgatgaagcg ccgcaggaga
    36961 ccggggcggc cgaaacggaa ccaacccccg cgccccccga gccgtccggt gatgccgagg
    37021 cgcccgcgca gtcgcgggac gagggtgata aggcgcctgc agagacaccg ccgcccgcgc
    37081 ccacgcccga accggaaatg acgcccgcgc ccacgcccga accgaccccc gcgccggccg
    37141 ccgagccttc gcctcaaccg gtccccaccg ccgaacccga ggttaccccc ggaccccaag
    37201 cgcagccgac gccgcaaccg gaagctgaac ccgggccgga gccgacgccg cagcccaccc
    37261 ccgccgaagc tccggacgag cccgccccgc cccccgctcc ggaaaacgac gatgccgcca
    37321 cgacgggctg accgcgcgac gctccaattt accgtgtcgg cctaccccct cgccgttgca
    37381 tcccgcagcg atgccaccgg tagggttcca ccccctcgcc gacccgtgcc cgcatcccgc
    37441 agggatccct tggagttggg cctttcgctc ggtcacgtgc agggatgctc gaggtggttt
    37501 tgcatcccgc agggatgcca gaaggtagcc ggtggttgag cgcagcgata ccaccggtca
    37561 cgatcgccgt cctccccgca tcccggcagg gatgccagat gccgcctcga agagggggaa
    37621 tctgtatcgg agaatcatac cagccccggg cggagcgcag cgcaacctgg ggacagccca
    37681 tgccacccca tcccccaaac ccctgcaaag ggtgcccgga gatgctggcg ccgcctgcgc
    37741 aggtatcgat cattcccgtc agaaaccacc ctccggcttt cgaaagccga ggacgagggt
    37801 cgagggccgg gagccgaatt tcgaggagac cttcggggcc gattaccagt cgcttcacaa
    37861 caagggagga agatgcaaaa ccgtaatcaa ccggtccaaa cggcaacagc cccgccggac
    37921 cgaccagccg gtcagtcccg tcagggacgg cagacttcag cccattactt cagtggcggg
    37981 aaagaatgga aaaccgtaat caaccggtcc aaacggcctc agccccgccg gaccgaccag
    38041 ccggtcagtc ccgtcaggga cggcagactt cagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    38101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38161 nnnnnnnnnn nnnngaccga ccagccggtc agtcccgtca gggacggcag acttcagccc
    38221 gccacttcag tggcgggaaa gaatggaaaa ccgtaatcaa ccggtccaaa cggcttcagc
    38281 cccgccagac cgaccagccg gtcagtcccg tcagggacgg cagacttcag cccgccactt
    38341 cagtggcggg aaagaggtga aggaaggaac gaagagtccc gcagggacgg cagaaccgct
    38401 cgacgttgcc agagaccaaa ccaggggggc ctaggccgcg atatcctcga gcgagtcgac
    38461 cagcagcgtg gcgacgttgt cgcgtacctc gaggaacccc ccggtcacat ggtagtaacg
    38521 ctcccccatg tggtcgcgga cggtcagctt gccgtccccc agcgtcgcga gcagcggcgc
    38581 gtgatgggcc agaacgccaa ggtaccccgt ctcggccggc acgatgatcg actcgacccg
    38641 gccttcaaaa accttgcgca cctgcgtgta gatctgaagt tgaaaatcgg ccatggtatg
    38701 attctcttat tcgaactctg ctggagagcc gaagcctgcc catccccggc atccgggacg
    38761 gttcatttac accttcgcca tccgttcggc gttggccatc acttcctcga tgccgccggc
    38821 catgtagaac gcctgctccg gcaattcgtc cagttcgccg tcgacgatca tcttgaagcc
    38881 ccgcacggta tcctcgcgct tcacatacgc ccccggccgg ccggtgaacg cctcggcgac
    38941 gtggaacggc tggctcagga agcgctggat ccgccgcgcc cgcgccaccg tcagcttgtc
    39001 gtcctcgctc agttcgtcca tgcccaggat cgcgatgatg tcctgcaggt ccttgtagcg
    39061 ctgcagcgtg ttctgcaccg cccgtgccac ctgataggcc tcggccccga ccaccatcgg
    39121 gtccagaatg cggctggtcg aatcgagcgg atcgacggcc gggtagatcc ccagctcggc
    39181 gatctggcgg ctgagcaccg tcgtcgcgtc caggtgcgcg aacgtgttgg ccggcgccgg
    39241 gtccgtcagg tcgtccgccg gcacgtaaac cgcctgcacc gacgtgatcg accccttgtt
    39301 cgtcgacgtg atccgttcct gcagcgcgcc catctcggtc gcgagcgtcg gctggtagcc
    39361 caccgccgac ggcatccggc ccagcagcgc cgacacttcg gatcccgcct gcgtgaagcg
    39421 gaagatgttg tcgatgaaca gcagcacgtc ctgccccatc tcgtcgcgga aatactccgt
    39481 catcgtcagc gccgaaagcg ccacccgcag ccgcgagccc ggcggttcgt tcatctgccc
    39541 gaacaccagc gccgccttgc tcttgcccgc ttcgtggctg accacccccg actcgttcat
    39601 ctccagccac aggtcgttcc cctcgcgcgt ccgctcgccg accccgccga acaccgagta
    39661 gccgccgtgc tgcttggcga tgttgttgat cagctccatg atgatgaccg tcttgccgac
    39721 gccggcgccg ccgaacagac cgatcttgcc ccccttggca tacggggcga gcaggtcgat
    39781 caccttgatc ccggtctcga ggatctgcgt cgccggcagc gtgtcggtca gctccggggc
    39841 ggggcggtgg atcggccagc gctgctcggg gtgcggcagc tcgccgcggc cgtccagcgg
    39901 atcgcccagc aggttgaaga tgtggcccag cacctggtcg ccgaccggca ccttgatcgg
    39961 cgcccccgta tcgaccgcct ccattccccg caccaggcca tcggtcggcc ccagcgcgac
    40021 gcagcgcacc gtgttgttgc cgacgtgctg cgccacttcc accaccagcc gcttgccgct
    40081 gtgcttgttc tcgatgtgaa tcgagttgta gatgtccggg agccggtccg aatggaattc
    40141 gacgtccacg gtcggtccga tgacctgctt gatcttgccg atgttcatcg cttgcctctt
    40201 gttggtttgc tattccgcct tcagtgcatt ccgaattcga tcgatcaatg gctgaacttg
    40261 tcggcgcgcc gccttgactt catcgctgct gatgctttcc tcaaacaggg taggnnnnnn
    40321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagcgct ttgctcatcg gctgcatgag
    40441 cggtcccttg cgaccgggtc aggccacctt cctttcaaat ttatagatcg gaacctcaac
    40501 ccggcggtcc ggggccgcgt gcgaatgcag gagacgatca agctccgccc caatcgctgg
    40561 gtggatgaac cattgcccac atttcgtgca gactcccgtg ggcgtatcct cgacaataat
    40621 cagtttttca ccagttcgaa gttctcgcgt ctggagctgc tcctcgatct ttccaccgca
    40681 ggtgtgacag ctcccgtagt ccctcttcat agttctgtcc ctcacgccag aatcgtctct
    40741 cgtcctcgtc ctcgtgatcg ctcccaaagg cctgcccgat tttatcccct cgttgtcaac
    40801 cgcaggttcg tgagcgactc ctcagcggtt tctctcttgc atgcgcaatg tcatcgtaat
    40861 ccagcccgtc cttcattctc tcttcatcag cgtgaagcga aagaatgaat tccttccgtg
    40921 ctccctgacg ctgaatttcg aataagtcct tctcaaacga ctcttcgctc atcgcgccgt
    40981 cgcccgcagg tcacttgggt tcccggctac cccgtcttca gcgcctccgc gccgccgacg
    41041 atgtcgagca gctccttcgt gattgccgcc tgccgcgcgt tgttcaggcg cagttgcagg
    41101 ctgtcctgca gatcgttgca gttcttcgtc gcgttgttca tcgagatcat ccgcgcgctg
    41161 tgctcgctcg tcgccatctc cgccagcgtg atgtagatct tcgaactcag gtagcgcggc
    41221 agcagcgaat cgaacacgat gtccgccgac ggatccagaa tgtagtcgac ctcctgccgg
    41281 gcttcggcct gccccgcctc gtccagccgg atccccagcg cctcgggcgt cagcggcagg
    41341 tattgcgcca ccgtcggccg gtagaccacc gtcgaaatga acgacgaaaa gaccagcagg
    41401 atctgatcgg tttcgtcccg cagaaaacgc tcgagcaggt agttggccag ctcccgcgcc
    41461 agcccgtcgt ccgcctggcc gaccagcccg atccagctcc gggccaccgg ccagctccgc
    41521 cgctgaaagt aatcgcgccc ccgccgcccg accagcacca gttcccactt cgccgccggg
    41581 tccccgcgca gcagttcctc ggtcttcttg atcaggttcg tgttgaacga gccggccaga
    41641 ccccggtcgg ccgtgaacag caccagcgtc ttgcggttgc cctcgcgcgg ctggaacagc
    41701 ggatgctcct cgatgaccga gctgctcgcc acatgcgcca gcagttcctg cagcttcgcc
    41761 gcatacggcc gcgccgccat cagcgtcgcc tgcgcccgcc gcagcttggc tgccgagacc
    41821 atctccatcg cgcgcgtgat ctgcttgatg ttcttgatcg agcggacccg ccggcgcagg
    41881 actttgagcg actctgccat cgactcagcc cttcgcctcg aatttctgct tgaactccag
    41941 caccgcccgc ttgagcttct cctccagctc cgctcccagc acccccttcg aatccaggtc
    42001 atgatggatg tcgtggtact gctcgtggat gtactcgttc agctcctgct cgaatcgcgc
    42061 cagcgcctcc agcggcaggt cgtccaggtg cccctgcgtc gccgcataca ggatgcagat
    42121 ctgatacgac tgcgcctgcg gctggttgag cccctgcttg agcacttcct gcacgcgcgc
    42181 cccgcgcgtg agctgctgct gcgtcgcgcg gtccaggtcc gagccgaact gcgcgaacgc
    42241 cgccagctcg cgatactgcg ccaggtccag ccgcagctgg ccgcccacct tcttcatcgc
    42301 cttcgtctgc gcgttgccgc ccacgcggct gaccgacaga ccgacgttga ccgccggccg
    42361 ctggcccgag tagaacagat ccggctccag atagatctgc ccgtcggtga tcgaaatcac
    42421 gttggtcggg atgtaggccg agacgtcgcc cgcctgcgtc tcgatgatcg gcagcgccgt
    42481 gatcgagccc cccgtcgtcg gatccatccg cacctcaagc tgctccttgt cggccatcgc
    42541 ctccagcgcc ttgcgcgcgg cttcctcccc cccctccgtg ccgcgataga ccttgccgtc
    42601 gactcccccc ttgctcgcgt cggccgtctc gcccttgcgc acgatgcaat acttctcctc
    42661 cagcttgcag gcgcgctcca gcagccggct gtgggcgtag aacacgtccc ccgggtaggc
    42721 ctcgcggccc ggcggccggc gcagcagcag cgacagctgg cgatacgccg ccgcctgctt
    42781 gctcaggtcg tcgtagacca ccagcacgtg cttgcccttc cacatgaaat cctcggcgat
    42841 cgcgcagccg gcatacggcg ccacatagag catcgtcgcc gggtcatccg ccatcgccgc
    42901 cacgacgatc gtgtagtcca tcgccccgtg gtcgcggaac gtcttgagca cccccgccac
    42961 cgacgacgcc ttctgcccga tcgccacata gatgcaaatg acgtccttgc cctgctggtt
    43021 gatgatcgca tcgacggcga tcgaggtctt gcccgtctga cggtcgccga tgatcagctc
    43081 gcgctggccg cggccgatcg ggatcatcgc gtcgatcgcc ttcagccccg tcatcagcgg
    43141 ctgcttcacc ggctggcgct cgaccacgcc cggcgcgatc acctccagcg cacggtagct
    43201 gtcggtcggg atcggcccct tgtcgtcgat cggctggccg atcggattga ccacccgccc
    43261 gatcatcgcg tcgcccaccg gcaccgacgt gatccggccc gtccgcttga ccgtgtcccc
    43321 ttccttgatt ccgatccccg gatcgaacag cgcgacgccg accgacgatt cctccaggtt
    43381 caggaccatc cccatcacgc cgccggggaa ttccagcaac tcccccgcca tcgcgtcgtg
    43441 cagaccatat accgtggcga tcgcgtcgcc caccttcagg atcgtgccga cgttctccac
    43501 gtcgatcccg gcctgatagc cggtcagctc gcgttccaga atttcggtga tttcgtcagg
    43561 ccgcagcgcc atgagctctc tccgctatgg ggtgtgtgtc tctcaatgtc gtccctcgtc
    43621 tctcgtcctc actcaaaaac ccgtcccgca tcctcgtgat cgctcccaac ggcccgcccc
    43681 gattttttcc cctcgttgtg aaccacaggt tcttcagcat cgcgtaatcg caatgacggg
    43741 ctgttcggag ccgggacgtc ggttgtcttc cttcccgcct gtcctgatca ggccgcctcg
    43801 tcccgcagca cggtcgcgcc ggccagccgc cggcggattt cccgcagccc cgcccgcagg
    43861 ctgctgtcga tcagcgtgtc ctggtagcgg aagacgatcc cgcccagcag cgacggatcg
    43921 accgcgtagt cgatgcgcag cttgctgccg gtgaacttct ccagcgacga cttcagccgc
    43981 accttctcct ggaagttcag ctcgcgcgcc gtctcgacga tcgcatgctg aatcccttcg
    44041 gcctcctcca ccagctcgcg gaacaggtcc aggatctcgt cgagcagcag gctccggtcg
    44101 cgctcgatga gcaggtggaa cagcttgatc agcagcggcg agagccggcc gccgaaaacc
    44161 ttgtcgatca gcgcctgctt gtcctcggtc gggacgcgcg ggttttcgaa aaagacgcgc
    44221 agttgcggcg tgcggccggc gatctcccgc aacacgcggc actcctccag caggcgttgc
    44281 agcgtcccct ggcgctgggc cgcattgaac agcgccgtgg catacgagcg cgagaccgga
    44341 ttgtagacgc gttgccggat catgcccgct ggcgctccag gtcatcaatg aaggccgcca
    44401 ccagccgctg gtcggtctgt tcgtccagct tctcgcgcag cagccgttcg gaggcgtcca
    44461 tcacgagctg gacgacttcg tcgcgcagct cgagccgggc ctggccgatc tgaagttcga
    44521 tgttgcgctg cgcccgctcg gtgatctgcc gcgcttcctc gcgcgcgttc tcggtcagct
    44581 ccgctgccac ccgccgcccc tccgcaatcg cttcctggat cttctggcgc gcctcctgtt
    44641 cgataccctc cagccgctgg cgatactcat ccagcgccgc ctgggcctcc tgctggcgac
    44701 gctcgatgtc ggcgaagccc tcctcgatct tgcgctggcg ctcgtcgagc a
//
LOCUS       DAOPCO010000007        43320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1014_length_43320_cov_6.161216, whole genome shotgun sequence.
ACCESSION   DAOPCO010000007 DAOPCO010000000
VERSION     DAOPCO010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 43320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43320
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1014_length_43320_cov_6.161216"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLS90_01225"
     CDS             <1..262
                     /locus_tag="PLS90_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HPK01053.1"
                     /translation="ILGVSVHSQPLRGANIAVRKAGIARVLAAAPIRVGAPVNIADTS
                     GRVKEVDEDTGAKVNCLGFAETAADNENDLVEVFIALHERTI"
     gene            314..1255
                     /locus_tag="PLS90_01230"
     CDS             314..1255
                     /locus_tag="PLS90_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01054.1"
                     /translation="MPEVHEVHIDAALTNLSVAYRNPAFIADDLAPVVPVRKQSDRYF
                     VLDPDREAFRSSDDRRAPGAEAHEVGFALSSESYYCEDHALASVIPDEERENADPVIQ
                     PNIDRTEFLRVKIDLNKELELAGLLTDPTLITQNETLADETQWSDPDSDPLAAVEAAK
                     ATVMAAVQALPNVLVLPYPVYAQLRLHPKVVERVVHGSVGAVDAELLAQIFDVERVLV
                     ARAFRNTAAPGQPPEMRYVWGNDALLAYVPARPALKQVALAYTFAWTGAPGALSGHRV
                     ELWREEKRKADVVRVQRYYDQRVIAPDAAFLWKEAVA"
     gene            1337..1762
                     /locus_tag="PLS90_01235"
     CDS             1337..1762
                     /locus_tag="PLS90_01235"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HPK01055.1"
                     /translation="MAYATLNELADRLGPELLLLVADDDGDGAADEPVIEAALTDAAG
                     EIDRALAGRYATPVADPPDLLRRWCIDLAIERLFVRHRDALPAEHAALAALARRALAA
                     IADGLAGLAGARPLARDHAAAGTHGGEGRVFDAESLADF"
     gene            1781..2386
                     /locus_tag="PLS90_01240"
     CDS             1781..2386
                     /locus_tag="PLS90_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01056.1"
                     /translation="MTAGIFTTGRAAETLLPAAVEPGSIHIAVAAHEFAPGDLLFAAD
                     DAGNHAQWLGRVSTRGPDSLNVTRPPSLALAPGMVLFRPRAGFLLDQGSVQLDSIAVD
                     PGIAVELTRDGAPHAAVLAEPATRVRLVLAGVSPRRAADFVLWLGEHAGWGLSAFTLV
                     GADAQPVALQLSGEPLEQSARGGSRCRLALPAILLAKDAVR"
     gene            2383..4155
                     /locus_tag="PLS90_01245"
     CDS             2383..4155
                     /locus_tag="PLS90_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01057.1"
                     /translation="MRPAAPLVVERSAHPRQRPVWLLEIETPGGAWRGASRPVSVGAA
                     PWPCRLGRVQFRERLPETGPPGGWIATDAEVDVIETPTTAGGLRAAAAPAALLGARAD
                     LALAWLDPEAPPDFSLNPGDRVTLLRGVVVEFQVQPASLRVRLADPLHARASRPVGRL
                     LHAALAGGEGPAVGRPLPWIFGRASRAELLPLRTGVATRLAADLAADATIVAVESLDG
                     FPPAGYAQVGGELILYHAVSTGGQTLGHAGSPVTREGVHAWTAGTEVRLRPPEGFVFL
                     AADHPCHAVTMVFGDDRPAPAGDWTADDWDLGGVAAQAVRLSRWPTDPAGWFARRICA
                     AVEGLELPDGTLMENPADVIQCLLTHPRLLGLDASRLDLASFAGEQARLAAAGYRYAR
                     RLTGAETLGALIAQAAAESALLVGGEPIRLLRLEPTSRTEDAELHLHDASVVDPRGRR
                     GRATSHLRIAPAGDAGLTPTVIELIGKAADGLQPASFSLPAGAAPAGAIPKRISFHWL
                     SFEAAGAVAEVAEVIGARLAAPRIAIDRIAAPRALLSSTGAAVLVTDAALGLDALVGW
                     IEDVTCVDFEVYVRIHAVGTAAAT"
     gene            4201..4755
                     /locus_tag="PLS90_01250"
     CDS             4201..4755
                     /locus_tag="PLS90_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01058.1"
                     /translation="MGWLEEFRAETAPVIAEPFFRCLAEAIAVVESAHGEQIIAGPEG
                     FNEIGYKALANHPHAVRETLEAGPRGELKRAQASFRLFRNRREQAQALLWLLRSSQFY
                     ESARLLFILCFYAAYAPGRRAGAVALLRVFNEIAAGGAHRGVRPFTLLTPEIDAGARA
                     LNHAAARRAVRLFGELTAARRVDD"
     gene            4773..5087
                     /locus_tag="PLS90_01255"
     CDS             4773..5087
                     /locus_tag="PLS90_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01059.1"
                     /translation="MDDQLLRQLNQRLDEHHAHLRERLDDLRREMQTLYGTIVARLDA
                     HETYHRQCEHRWGLPRLAQRHPFRLALIAFSVAWLMAAAAPESLQWLTRLALDWLRSL
                     RG"
     gene            complement(5231..6385)
                     /gene="cfa"
                     /locus_tag="PLS90_01260"
     CDS             complement(5231..6385)
                     /gene="cfa"
                     /locus_tag="PLS90_01260"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HPK01060.1"
                     /translation="MLTGELTQAPAGRIPRGRALIDAILERADVRIDGGRPWDIQVHD
                     PRFFNRVLAEGSIGLGESYMDRWWDCAALDELIARILRLDLSRRVQRNLRNAATVLWA
                     RVANRQTRTRSREVARRHYDLGNDFYAAMLDPYMQYSCGYWKEAENLAAAQEAKLELI
                     GRKLALKAGERLLDIGCGWGGLLRFAAERFGVEAVGVTISERQAELARARCAGLPVEI
                     RLQDYRDLREPFDKIVSVGMFEHVGHRNYRLFFEVARRCLKREGLLLLHTIGGNRSES
                     CIDPWIDRYIFPNAMLPSARQIATAAEGLFVMEDWHNFGPYYDRTLMAWFDNFNDNWP
                     RFREQYGDRFHRMWEFYLKSCAGGFRARVNQLWQIVYSPCGVEGGYTPVR"
     gene            complement(6730..7983)
                     /locus_tag="PLS90_01265"
     CDS             complement(6730..7983)
                     /locus_tag="PLS90_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK01061.1"
                     /translation="MIVSPHNEFIRQEFERGFQRWYAERRGGAPAVEFEWQDRGGTYQ
                     MVEAIAGAYQALDDKDDGMGVDLVWGGGIEAMEQLKAAGATTAAALPQPVLDSLPAEI
                     AGVRLYDPDGHWYGAVLSSFGIIYNKEVLRTLGVAAPATWSDLSNPALFGHIVLADPS
                     KSGSARVAFEVILQKHGWAEGWALLMEIAGNTREFTDSASNVPSEVAQGSAAAGMSID
                     FYAFSQIESTGAEMLGYVSPVNATALTPDPICMLRGGPNPAAAEAFMEFVLTKPGQSL
                     WLLKPGTPGGPAEHGLWRMPILPAAYEHPADQRLIDFNPYASETTFNLDMDLSTRRSP
                     LLGPLFMAAFLRNSELLRQAWRKVMTLPADAPLRRQFNAVPFSEEEALDLARRYQASP
                     REASQLEIDWQRRFRARYEQVLNAE"
     gene            complement(8257..9330)
                     /locus_tag="PLS90_01270"
     CDS             complement(8257..9330)
                     /locus_tag="PLS90_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01062.1"
                     /translation="MQPVRVENLVKQYGALRALDGVSLAVEAGELFFLLGPSGCGKTT
                     LLRAIAGFVPPDAGRIEIGGRDATRMPPQARNTGMVFQSYALWPHLTVRENVAFGLRL
                     QALPRAERDARVDEALAVVRIADQAGKKPNQLSGGQQQRVALARALVIRPACLLLDEP
                     LSNLDAKLRAEMRGEIRRICKASGLTAIYVTHDQKEALAIADRIAVMRAGQIEQLGPP
                     RRLYHHPVNRFVADFLGHTNFLRGRVTGAAAGDRVVVATPVGPLHARADGAPPAGEVL
                     LGIRPEALSRRHAAEVNVFEARVTAVSYLGEVTQYDLATEGGEPRALAALELNAGEEV
                     LAEGARVRWHVAPEHVMLLPAED"
     gene            complement(9595..10623)
                     /locus_tag="PLS90_01275"
     CDS             complement(9595..10623)
                     /locus_tag="PLS90_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HPK01063.1"
                     /translation="MENVLFLACIEPDGSLGKTARETLAIARQAAAELGAEFCAGLIG
                     EHCEPGSRTAAAAGCAALYLVEGSAFGISRYATDSAAAEAIARAARPTLVFAPAVSRV
                     SRFIAGLAQRLGGRVDTQITGLAVHDGQLVVDRWFYRQRIEATLTRTTRPWLLTVAPG
                     TMPAFDGDAAPTRIERIAVDLPESLQRTRVEGVQAPPGAGQTVRPDAELLFVAGAGWT
                     KPQRDGQSHVEQAAQLILGFLDRTQASLGGSKSLVDVGGEGQPVLPFLTHLNQVGQTG
                     ATPRHPKGLATCCHGEEPHVVGWRFINERRAINLDPNCGWARGKADVLYVADAFAVMR
                     KVNELLGG"
     gene            complement(10685..11491)
                     /locus_tag="PLS90_01280"
     CDS             complement(10685..11491)
                     /locus_tag="PLS90_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPK01064.1"
                     /translation="MSGYHVVVCGSVVPDPLHPLEPVTGPNGPELKNEMILPAVFDPW
                     AAHALYEAAHLVQSVPGSRLWLVSIGPRAKLQQVMMTIGQKVVLQLVALDGPAGGFVD
                     AAATAEALADAIAAIDGLDRARLVLCGGWASASRGAGAVLQMVGEHLGIVDQFQGVDE
                     LAVQPDGALRVVERADGGLNQVSLCAGPPVLLGWATGNRPEPPNNPQIGMQNMRGVMP
                     ALQKARPAQLGMDGLRYCCAQAPRRRRETRIVKDMPVDEIAREIVAWIRG"
     gene            complement(11692..13560)
                     /locus_tag="PLS90_01285"
     CDS             complement(11692..13560)
                     /locus_tag="PLS90_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPK01065.1"
                     /translation="MNALASPTPAVERQTMEVDIVCVGFGPAMGGFLTTLANKLSDPR
                     TAAALESRAMPGLPLQVVCYERADDIGFGVSGVATRGRAIRASLPDLDLNQLGMAAPV
                     TNEKLVYLLDPHGASRRPRLMRLADRALRRLPRVRRDWAFELPYIPPFLRKDDGFVMS
                     LGQFNQWVGARLMGTGAVQIWPATPVAAALTAEGRVHGVRLIDQGVDRRGRPEPGYLP
                     GMDIVSRLTVVGDGPVGAVGQQLDRELGLPPDHERNDWAVGMKMVVDLPESCALEPGT
                     VIHTLGYPEPELFGFLYVMPDRVASLGIFVPSWFDSPVRTSYRYLQHWMRHPYLWRHL
                     EGGRLRSWGAKSLQESGRRGEPWLAGDGFARIGEGSGSTNVLTGSGVDEAWATGCQLA
                     EAVAELWRAGKECTRANLEAAYVARRRRSWVDEESRIAERARDGFQRGFIPGLLGMAL
                     TGLTRGRLAWPGRSVPPHEGIAALEEFHAGRIPPDRIARIRRECRASGRPLHDALMDA
                     AGWPPVEYDGRLLVSHQDALLLGGKVQAPAGYADHVVFPFPELCAECGAPVCVEICSG
                     QAITPNPGGGAPLFDREKCVHCGACLWNCSQSFPEDPHRGLLVFRAGAGGLHSAEN"
     gene            complement(13741..15942)
                     /locus_tag="PLS90_01290"
     CDS             complement(13741..15942)
                     /locus_tag="PLS90_01290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPK01066.1"
                     /translation="MDQMLTLPGDDIRQLMWRFSDRFDYQMLIQATRAVARGPVARLV
                     AEGARNTHEWTEAKRGLLSAFDDAGITAAGIDPEFGGYLAGPKNFVLAMLAFELAWVD
                     AGAATCSLAGDLALAPIHQRGTPEQKAAYMSAVVPGAARRQRGAFALTEPIPFVGVDT
                     GVLGGKMRIHDWPADVEPTLEVEKRGRFITNMGFATFVTAAVESDDPRIKGSCMVILE
                     ETDPGLFDRGLPTRKLVHQLSSTRDPILNLRVPASRIIGGYTVRDGVLIPNYTHGEVI
                     EAVFRRTRVTVALMTAAKLLSAIEPIIRYHRTRFRGGDAGEPGSVRHEMGLQQKEDAL
                     HRLIDVWATGESAASLGFEAARLFDQFDPLEARKLELFAARGLTGRHQLKEMRRLNEA
                     ALELIELEGLPEAERDAARFDQLQQDELVQFARLDALTNVLCPAAKLWNTGHGANMMR
                     EAVSLMGGYGITEDCPGFLGQKWMDAQLEATYEGPEAVQRRQLTVTMTNEVFLAQFRQ
                     WIGELRRIGSQTPDSGACGLATAMQMWLWTLNHLRRARDAEGQLLYQSRRQGVTFPLA
                     DALCWLLATRSLILDVEELKARAGENPVLAENAAGIARFYTDLCHVQAARACGEVGRI
                     CAELVYGYNAHPAWDAASCDACYDMGELDEMESVMPGIGGTAVAYADVKVKGEVHPPK
                     AGPCVRFDGMNRFLRLRLRMDGCLTGSRLAKDRLAHNLARIMIPEALDYPR"
     gene            complement(16548..16976)
                     /locus_tag="PLS90_01295"
     CDS             complement(16548..16976)
                     /locus_tag="PLS90_01295"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK01067.1"
                     /translation="MSARVPIHRRRRLQAGVNLTPMLDVIFNLIFFFILATTIREETF
                     EMSIQLPESSTVTAAAGETIPHVAVSAAGDIFYGDRRVVEEELELEMRALAGEGQRAV
                     LVRGDTAVNLGRVIEVFDICRRAGLVEAQLETRPPAPTSP"
     gene            complement(16973..17923)
                     /locus_tag="PLS90_01300"
     CDS             complement(16973..17923)
                     /locus_tag="PLS90_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK01068.1"
                     /translation="METLLIKYVINGGPVMFLLVPCSLLMLGAVIQGLIRLRSGRVLP
                     GWVTRQAESVKSPDMRRRFVIGLSESRAPLARAVWLTLRLHNVAQTRRPPQQELLISM
                     EEASAEVGDEMRDGLALLATIYTIAPLLGLLGTILGMMNTFYVFAQSTERSVKLLSIG
                     IQEALVTTLWGLGIAIPAYVAAQWLERIIADYERRRLPEAVSRVLEAIYGPPRDEEEP
                     AATPRPVPAPVVAAGPARPVVSPPEPRAPARPAAEPRPATRPGAPSPAAARPAAQPGG
                     IAARPAAVPGQARPVARPAAPAQPATGPAPAAPRPDEAGR"
     gene            complement(18069..19340)
                     /locus_tag="PLS90_01305"
     CDS             complement(18069..19340)
                     /locus_tag="PLS90_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01069.1"
                     /translation="MKPLPVREKEYRQGLYKLERTAIIPFKWVILVVTLVLWVWIIKR
                     PPSTIVFMLFVLYFLFNSAQTYFFYFSEVANHQIRPLVLFSYLVDVVFVSMLIYLDLA
                     TTSFGVETHHDFYVLYFLLVMRGFALFKTIAETIFVNLLISILYFITFYVPGYDLDAN
                     FAVSLVLIWLVILMSWFIVMIITRQKTELLEANDRLLRADNLARVGELAAGVAHEINN
                     PIGIIAATTDYLKRVLPADSEAAEEIEAIHREAMRCKRIVQEMLTYANPRPSGTTPLD
                     LKELNEEVLRFVFPRRQTEKLLIVTEYTPEPTIVSVDPNQLKQALLNLYLNARQAIPE
                     GRVGRIVIRILNQPRAGRARIEIEDNGIGIPEDDLDNIFEPFFTRKAHGTGLGLAVTQ
                     RIIENSGGSISVRPAPEQGTVFMVDLPLAER"
     gene            complement(19337..20215)
                     /locus_tag="PLS90_01310"
     CDS             complement(19337..20215)
                     /locus_tag="PLS90_01310"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HPK01070.1"
                     /translation="MTSPADTASAPAPAAGPLVLVTRPDGENRALGDAIRAAGFDSRL
                     TPVIQFADCPAADDPALADRIRRLDRARGWLILPSPTAIGRLAQALRRRGVDPRELAG
                     LRLAVVGAGSNRRLREHWGREADFVPTLPTAAELAAGLPAEPRQPILIVGSRQSRPEV
                     PRGLRRRGRLVESIVLYETLPCPEGLSALREALGESARQARPPVLVVASPSAVEAIFG
                     FFGAAARLPRAGWITIGPTTHARLLRRTAELEEPPPTVQSESPAPEAIVQAIRRIQGR
                     LGSRAALPPRAGDPRP"
     gene            complement(20212..21168)
                     /gene="hemC"
                     /locus_tag="PLS90_01315"
     CDS             complement(20212..21168)
                     /gene="hemC"
                     /locus_tag="PLS90_01315"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPK01071.1"
                     /translation="MSPPALKTHLRIGSRGSELARWQADYIAERLRALGCTVEIILIK
                     TRGDLDQRTAFGGMNAKGIFTKEIEDALLAGTIDLAVHSLKDLPTQLPPGLELVAVPE
                     RESPLDGWVSRHGTALADLPPGARVATGSLRRQAQILALRPDLEVVPIRGNVHTRLQK
                     IRDGLADATLLAQAGVHRLGLSDVLTSVFSPDQLTPAMGQGALGIEARAGEFTDIWPQ
                     LEHIPTRLAAEAERAFIGAIGGGCKTPAGVLAQPREGGGWRVTAVLASPDGRHLLRRG
                     AVCDHDDELGPLALALARSMLAEASPEIRATLSPAEPPAGAP"
     gene            complement(21410..21892)
                     /locus_tag="PLS90_01320"
     CDS             complement(21410..21892)
                     /locus_tag="PLS90_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HPK01072.1"
                     /translation="MFNISKLFGRGEDEPARDLENEPPAPAPAPAATPTDETGPAPGP
                     DSAAASAPADPYHADTEALREAIIQVIRTVYDPEIPVNIYDLGLIYNIDVDPTGFCHI
                     RMTLTAPNCPAAGYLPGEVEQKAALVEGIRGVTVDVVWEPQWNQSMMSDAARLELGIY
                     "
     gene            complement(21899..22348)
                     /locus_tag="PLS90_01325"
     CDS             complement(21899..22348)
                     /locus_tag="PLS90_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HPK01073.1"
                     /translation="MADLDDLYREMILDHSRNPRNFRSNEAAAGIAHGNNPLCGDRVS
                     VFVDLDGDSIRDISFQGSGCAICTASASLMTQALKGATRRRAQFLFENVHHMLTDGKL
                     PHPEVELGKLHVLEGVREFPIRVKCATLPWHTFHSALTRGSEIVSTE"
     gene            complement(22348..23622)
                     /locus_tag="PLS90_01330"
     CDS             complement(22348..23622)
                     /locus_tag="PLS90_01330"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPK01074.1"
                     /translation="MSSAVPLDALRDTHAPRVEPLDVERVRQDFPILQRKIRGKPLVY
                     LDNAASSQRPRVVIEAIRKLYEQDYANIHRGVHTLSQVATEAYENSRDKVRQFIGAAD
                     RREIVFTRGTTESINLVANSFARPRLQPGDEILITAMEHHANIVPWQLVCEQTGARLR
                     VAPISDEGILRMEEFARLLTPRTRLVAAVHVSNALGTINPIRRMIELAHAAGIPILID
                     GAQAAPHLRVNVQQLDCDFYAFSGHKMCGPTGIGVLYGKLAHLEAMPPWQGGGDMINV
                     VTFEKTTYNDPPQRFEAGTPNIEGGIALAPAIDYLLGLGMDRIAAWENELLAYATQCA
                     ADIPGLRIIGTAPCKASVLSFVLDCAHPHDIGTILDQEGVAIRTGHHCAQPVMQRFNV
                     PATARASFSFYNTREEVDALFRGLRKVLEVFG"
     gene            complement(23619..24968)
                     /gene="sufD"
                     /locus_tag="PLS90_01335"
     CDS             complement(23619..24968)
                     /gene="sufD"
                     /locus_tag="PLS90_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPK01075.1"
                     /translation="MATNPPGLTGQDYFLQATALAAQGRGQEPAWLGELRRAGADAFA
                     RLGFPTRRMEGWRFTSLDPLLDQAFAPAPAADGERAIADKIQPYLLGDLPAHRLVFVN
                     GRLLPALSDIAGLPAGVVLTSLREALRERPEEVRSRLGRLAPHAEHAFIALNLALHED
                     GVYLHVPRGRQIDAPIHLIFFSSGEPAFHAHIRNLLVLDAGARATLIESYAGAEDHAC
                     FNTSVAETWLEPDAWFDHYKLQEETRETIHVATHHVHQARASTFATHTISIGGGLIRN
                     EINPVLDGEGIESTLNGLYILDGAQHLDNHTRFDHARPNCRSTQLYKGILDDRASAVF
                     TGRILVREDAQKTDAFQQNRGLLLSPQASLNTQPQLEIFADDVRCTHGATIGQLDPEA
                     LFYLTTRGIDLQAARGLLTYAFANEIIEQIRIEPIHHRLEREIIRRFRGEEQGGVAA"
     gene            complement(25014..25772)
                     /gene="sufC"
                     /locus_tag="PLS90_01340"
     CDS             complement(25014..25772)
                     /gene="sufC"
                     /locus_tag="PLS90_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014702905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPK01076.1"
                     /translation="MALLEIRNLHASVDGKQILKGINLTVNGGEVHAIMGPNGSGKST
                     LANVLAGREVFNVTQGEVFYDGRNLLEMDAAERACAGIFLAFQYPVEIPGVGNLYFLR
                     TAYNAIRRYRGEEEIDAADFLKLVREKAALLEMDERLLNRSLNEGFSGGEKKRNEIFQ
                     MAVLAPRLAILDETDSGLDIDALRIVADGVNSQRGPDRGVIVVTHYQRLLNHIVPDFV
                     HVLADGRVVRSGGKELALELEDKGYAWVEQPAGV"
     gene            complement(25783..26829)
                     /locus_tag="PLS90_01345"
     CDS             complement(25783..26829)
                     /locus_tag="PLS90_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPK01077.1"
                     /translation="MPVQWLGIALWLGFILFILLMLALDLGVFHRHDRVIRVREALGW
                     SAFWICLALIFNVLLYFYWDDLHPFGSWGAQYTPREAALMFLAGYVVEKALSVDNLFV
                     ILMIFSYFRTPRVYQHRVLYYGILGALVFRFIFIMLGAVILHRFFWMTIVFGLFLIYS
                     GVKMMLVKDHEIEPEKNPVIRLFKRLVPVTQDYRGKHFFTRIDARLFATPLFVSLLVV
                     EVSDIIFAVDSIPAIFAITHDPFLVFTSNAFAILGLRSLYFALDGMVQLFRFLSYGLS
                     LILIFIGGKMLYNYVEQEVMTDWGHFPIGVSLGIIGILLAGSVAASLLIPAPKPASLA
                     PEPEKQRSLRTESE"
     gene            complement(26922..28379)
                     /gene="sufB"
                     /locus_tag="PLS90_01350"
     CDS             complement(26922..28379)
                     /gene="sufB"
                     /locus_tag="PLS90_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPK01078.1"
                     /translation="MSEATTSTTILDELTRRQYQHGWATEIEADSLPPGLDEAVVRAI
                     SARKHEPEWLTEWRLGAYRHWTGMQEPHWPNVHYPKIDYQAISYYSAPKKKSDAPKSL
                     DEVDPKLLETYEKLGIPLHERELLAGVAVDAVFDSVSVATTFKEKLGELGIIFCSFSD
                     AVRDHPELVRQYLGSVVPRTDNFYAALNSAVFSDGSFVYVPRGVRCPMELSTYFRINA
                     QNTGQFERTLIIADEGAYVSYLEGCTAPQRDENQLHAAVVELIALEGAQIKYSTVQNW
                     YPGDEEGRGGIYNFVTKRGLCKGAHSKISWTQVETGSAITWKYPSCILQGDHSVGEFY
                     SVALTNNRQQADTGTKMIHIGRDTRSTIVSKGISAGRGQNTYRGLVQIHKKAENARNY
                     SQCDSMLIGDRCGAHTFPYIDVRNPTGQVEHEASTSKIGEDQIFYLNQRGISTEDAVS
                     MIVNGFCKEVFRELPMEFAVEAQKFLGVSLEGSVG"
     gene            complement(28414..28896)
                     /locus_tag="PLS90_01355"
     CDS             complement(28414..28896)
                     /locus_tag="PLS90_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HPK01079.1"
                     /translation="MLRLTKAADYGIILLTYMGWKSAEPHNARDLSRAAHLPLPMVSK
                     ILKSLARAGILESQRGTKGGYRLARPPARISVAEIITALEGPIALTECVGEVESNCSV
                     ERLCPIRVSWHRINHAIRDALAEITLDEMMGPMFPRPADRTAAAGAPTLSLTAAAEAD
                     "
     gene            29163..29669
                     /locus_tag="PLS90_01360"
     CDS             29163..29669
                     /locus_tag="PLS90_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01080.1"
                     /translation="MVDSTGKSLWKVGAKFGVNGAHWGDYDGDGTDEMVVGMNGMGGL
                     QAWSADGQKLWSVRLGNVWDQAIVPASEGHPARILATEAGGAVRVYDAQGNPLAPLRP
                     RGAYYAGMSACRTTGGAVQIAAQSDETTVFFRRSGQDRVDGHRAGRLRRVAGEQPGRG
                     RCGRRWSA"
     gene            29684..29851
                     /locus_tag="PLS90_01365"
     CDS             29684..29851
                     /locus_tag="PLS90_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01081.1"
                     /translation="MARDGWWWHRRRASNFTALAHAADVEGFAIASRAPEPGVLVVLA
                     ESMLSACTLAR"
     gene            complement(29939..30310)
                     /gene="mscL"
                     /locus_tag="PLS90_01370"
     CDS             complement(29939..30310)
                     /gene="mscL"
                     /locus_tag="PLS90_01370"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPK01082.1"
                     /translation="MGFIQEFKEFAVKGNAIDMAVGIIIGAAFNKIVNSLVNDIVMPP
                     LGYLAGRVEFKDLGYVIRPAMTDPTGAELPEVAIRYGQFINTIIEFLIISFTVFIVVK
                     GMNRLIRKREAEAAVEEAKAS"
     gene            complement(30747..31877)
                     /gene="mutY"
                     /locus_tag="PLS90_01375"
     CDS             complement(30747..31877)
                     /gene="mutY"
                     /locus_tag="PLS90_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPK01083.1"
                     /translation="MDAPPTAVPPEAVAAIRQRILRWFDEEHRDLPWRSRRSEYGTVV
                     SEFMLQQTQVATVVPYFNAFLEQFPSFEALAEASEEDVLRAWSGLGYYRRARMLKRVA
                     ESVVRRHGGKLPRTREELAELPGFGEYTVGAVGSIALGLPLPLVDGNVRRVIGRLFAL
                     EEDLTRAVGRKKLWALSRALVDPVRPGDFNEGLMELGATVCVPREPLCLVCPLFESCA
                     ARASGLPEAYPRPTPGMRIRQVREVAVVLVRKGRVLALQRGETGAFAGMWELPRLDSR
                     EIGIPENLTPDTVLFELARLRIGQAAPEFLGSTRSTFTHHVITTELYRVELAAERGPV
                     RRQHHVAHRWLVPRSLHELPASRSQHKLFELVVESFKSGGAD"
     gene            32022..33089
                     /locus_tag="PLS90_01380"
     CDS             32022..33089
                     /locus_tag="PLS90_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01084.1"
                     /translation="MIERDTGDVNRTIPAAAADDPPARLEDHRVLPRLLAELLAGREL
                     HGGLDRDDLREMSKRAERPNIWGLPLHPIQRPWPGELDLNGRMGPDGPLAIGPRPELP
                     IGVFKSFQRQLRQTTYVFVRGWVGGASADPLAVAGIFPQEAAHLAAGLQELLWPMEPA
                     SEAAWASRPAHYTFLAWPDAIRGRDGQPRVEMLIGHWPGEGVGLIVGCDDPRALEEVA
                     RDAARRRWSARRDVELIRGALDCGGEVARLVRDATETGGALIEEPLVVERGRVSPLWR
                     NPLLPADRLSRADWRAAALDPWALPFGVCDNQEGHLPLLQPEYDEATRRAAAGGWVVV
                     PRRAAPPPGPSRPMAYRLREA"
     gene            complement(33521..34618)
                     /locus_tag="PLS90_01385"
     CDS             complement(33521..34618)
                     /locus_tag="PLS90_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01085.1"
                     /translation="MFPDPEATLGAAAATRDRLAEGELLLEVNDLRTHFITRDATRRA
                     VDGVSFRIRQGRTLGMVGESGCGKSVTSLSILRLVPSPPGRIVGGEIRFKGRNLLTRT
                     EKQMRRIRGNEISMIFQEPMTALNPVFTVGSQVAEVFRVHRGLSRRDAFDEAVRMLQQ
                     VRIPDAERRAREYPHQLSGGMRQRVMIAMALGCAPDLLIADEPTTALDVTVQAQILNL
                     MEDLQRKTGTSILMITHDLGVIAETSDEVAVMYAGQIVEFAATEELFASPLHPYTQGL
                     MRSVPRLEWVRAGQSIEPIPGNVPDPAHFPPGCRFHPRCPFAAPVCVAQTPELEESAG
                     GHSVRCLRWREILNNPKSARASASVAHEQGS"
     gene            34823..35389
                     /gene="efp"
                     /locus_tag="PLS90_01390"
     CDS             34823..35389
                     /gene="efp"
                     /locus_tag="PLS90_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPK01086.1"
                     /translation="MVAIDANDARPGTKLLIDGELYNVVERAHHKPGKGGAFVKFKLK
                     GLRTGKVIDQTVRAGTTMEQADIINQTMQYVYQENDQFIFMDLQTYEQMPVPASVVGD
                     AAQYLIENTDVQVTLYEGQVIGLQLPPKMDFTVIETIDDAVRGNTATNVTKDAKIETG
                     LIVQVPMFVKTGEKIRISTEDGKYIERA"
     gene            complement(35507..36796)
                     /locus_tag="PLS90_01395"
     CDS             complement(35507..36796)
                     /locus_tag="PLS90_01395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK01087.1"
                     /translation="MLVVVVLTAAGGWLRAREIERRGPLAPPDQKLDFDAWYYYVSAG
                     QALLEGRGLTPAYNERDPPPVYLVPPPLQALFTAAVYAVRGAFDPAALVWTQLGLSLL
                     AGGLVYWLLARWHGVAAALCGVAIWMLYPEFIYWVATPMTEANYLVLLLLVLALLAAW
                     CDRRATGWALAAAAALGIANLQRPNGLGWGLAVAAFALWRLRGDERRWLQATIFAAAP
                     FLVLLPWLARNVLVTGDPVLVSSNGGVLLYAVNHPGYDPLVWPDYGVYPAAEWHAQPI
                     LDRFNSAEFDGRRTYYQLSKLYAREVRAYVLAEPLHFLRNYVIKLWQRFALVPRVLAE
                     VDPWLGERTAALLYHGLTLLGLAGLIGRRREWRNPRHVLLAISFGWFAAFAALMHWLG
                     SGRMTITLRLFLMLFAAVFLGACLNGLTQKLASRPSR"
     gene            complement(36922..37218)
                     /locus_tag="PLS90_01400"
     CDS             complement(36922..37218)
                     /locus_tag="PLS90_01400"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HPK01088.1"
                     /translation="MAHELEFIVLDAAALAEALKSLPGWESDGQAIKKQYEFPSFMEG
                     IQFVNRVAETAERFNHHPDIIINYKKVTVRFWTHKKNAVTRADVLVAGEVERVA"
     gene            37380..40232
                     /locus_tag="PLS90_01405"
     CDS             37380..40232
                     /locus_tag="PLS90_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01089.1"
                     /translation="MAGPIRHHPAMSRALPAWAPWLGLVALSLLFYWPLALGRVPMGA
                     DNTLFYAPYYVFRWDDGLPLWNPCLLAGTPLFNDLQAALLYPLRWPFYFLADWRAYYG
                     LFNYLHYLVAFAGGMGFLRAAGIAPWPALAGAMLFAAGGHMAGRIINPTIFHASCWLP
                     WILWAGAGVGARHRWLTTLAWAMVLTIASPHLMFYGVVAMAAVWIPLAAGPLRRARGW
                     RAVAGDAAARVGHFGLAFALAAPTWIPGVLRARDSIRSTTSAMDNLANSLGWGEIPRA
                     LLGGTGGAVYPEFIDKSLYIGPVALALIVGWAARRAAWRDPRTWSGAALVLLGIGFAL
                     GRHIGIQFVMPWVPGFNLLAGPARALVLAAAGLGLLLALALADLWREGDPAAHGRRRR
                     VGVLALTVGLVCIVVFTWRAWGRAASEGSAPFAEDWLAAWLRAPKAVPPELQPWLEAA
                     VALPAAGIVLLLPAMRRRAAACLMAVLLAGLLQHFVPRVAPPYETAEDRRVPESIAWL
                     TRQHEAAARAGRPFRVAGYDPLRLHDTEMDSRFRAATLMPNLATLYGLEDIHGFNPLI
                     TLDYLELFLRTAGRSPINDPYRNLTIDRPDPRLFQILNLRYLAGHPYERRLTSTPQTL
                     RPQAATQPVVDWEDRGTSEPIVAWLFVSLLDGAGADPATLPIGAEVARLLVEAEEGRF
                     AFPVRNGVETAWLHAPSRPELLRRDDFSATVNDLWSTHIAAPALDDRLATANYRGRIE
                     FGRPLHVRRLAWELAQPGLVFHLAGQAYRIAPPPEEEEPWRLAYGAHSDWAPIFEFRA
                     ATPRFALWEPAAAQPAPSTAPPRPLPGVAEGDGVALLERGNHTLRLRVEAPRPALLVL
                     REIWTPGWSAEVNGTAAEVIRVHEVLRGVPVPAGRSDVTLVYRPASFYVTLLCALPFH
                     LVGLGVAARDLRRWRARSGGRGNEGAGWMGRKGWEK"
     gene            complement(40584..41036)
                     /locus_tag="PLS90_01410"
     CDS             complement(40584..41036)
                     /locus_tag="PLS90_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01090.1"
                     /translation="MVTTPEANMHILLVEDNPGDIRLVREALAMARLQCQLHVATDGD
                     EAIDFLRRRGRHAQAPRPHLVLLDLNLPGRHGREVLREAKLNPELRSIPVIVLTTSQS
                     EDDVVYAYDLYANCYIAKPSNLDEFMGIVKMIDNFWFSMVILPHGEGT"
     gene            complement(41042..42211)
                     /locus_tag="PLS90_01415"
     CDS             complement(41042..42211)
                     /locus_tag="PLS90_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01091.1"
                     /translation="MDPHMLHHTEYMAHGFCLLWERPLVWLHVVADSFIALSYYSIPL
                     MLLWLARRRTDLVYHWMFLMFAAFIFACGTTHVLSIVTLWKPWYWLDGLVKAATAAIS
                     FTTAVLLLPVVPRTIRIPGTRQLTQANEQLQQKIAELNQTQAQLQRYAIELERSNQEL
                     GEFAYVASHDLQEPLRMVTSYLQLLEARYRNQLSDEADEFIHFAVDGASRMQGLINDL
                     LAYSRVTTRARAFSPVEMNEVLRRAQHNMQVAIAETGARIDADDLPRVNGDSTQLVQL
                     LQNLLSNAIKFRGKQPPRVELRARREDDHWRFSLRDNGIGFDARYAEQAFMIFKRLHS
                     NREIPGTGIGLAICRKIVERHGGRIWVESVPGEGTCFHFTLPAITVKPFDPGDSE"
     gene            42715..43221
                     /gene="purE"
                     /locus_tag="PLS90_01420"
     CDS             42715..43221
                     /gene="purE"
                     /locus_tag="PLS90_01420"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007507480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPK01092.1"
                     /translation="MNAPSAPLVGIVMGSRSDWPTMEHAAAILTQLGVPHEVSIVSAH
                     RTPDRLFQYAESAAGRGLEAIIAGAGGAAHLPGMLAAKTVLPVFGVPIQSRALNGLDS
                     LLSIVQMPRGIPVATFAIGEAGAANAALHAAATLATHHPSIREALEAYRRRQTQEVLD
                     HLDPRESS"
BASE COUNT         6951 a        14150 c        15418 g         6801 t
ORIGIN      
        1 catcctgggc gtctcggtgc acagccagcc gctgcggggg gcgaacatcg ccgtgcgcaa
       61 ggccggcatc gcgcgcgtcc tggcggcggc gccgatccgc gtcggtgcgc ccgtcaacat
      121 cgccgacacc agcggccgcg tgaaagaggt cgatgaagac accggggcga aggtgaactg
      181 cctggggttc gccgagaccg ccgccgacaa cgagaacgac ctcgtcgagg tcttcatcgc
      241 gctgcacgaa cgcactattt gagcgcccgc cgcgctcaat ccctcacctc tctgaagcaa
      301 aggagtgacc gccatgcccg aagtgcacga agtccacatc gacgcggcgc tgaccaatct
      361 ctcggtcgcc taccgcaacc ccgccttcat cgccgacgac ctggcgccgg tcgtgccggt
      421 ccgcaagcag tccgaccggt atttcgtcct cgacccggac cgcgaggcgt tccgctcgag
      481 cgacgaccgg cgcgctcccg gcgccgaagc ccacgaggtc ggcttcgccc tctccagcga
      541 aagctactac tgcgaggatc acgcgctggc ttcggtgatc cccgacgagg aacgggagaa
      601 cgccgatccg gtgatccagc ccaacatcga ccggaccgag ttcctgcgcg tcaagatcga
      661 cctcaacaag gagctcgaac tggccggcct gctgaccgac cccacgctga tcacgcagaa
      721 cgaaacgctc gccgacgaga cgcagtggtc ggaccccgac tccgatccgc tggcggccgt
      781 ggaggcggcc aaggcgaccg tgatggccgc cgtgcaggcc ctgcccaacg tgctcgtgct
      841 gccgtacccg gtctacgcgc agctgcggct gcacccgaag gtggtcgagc gggtggttca
      901 tgggtcggtc ggcgcggtcg acgcggagct gctggcgcag atcttcgacg tcgagcgcgt
      961 cctggtggcg cgggccttcc gcaacaccgc cgcccccggc cagccgccgg aaatgcgcta
     1021 cgtctggggc aacgacgccc tgctggccta tgtccccgcg cggccggcgc tcaagcaggt
     1081 cgccctggcc tacaccttcg cctggaccgg cgcgcccggc gcgctcagcg gccaccgcgt
     1141 cgagctgtgg cgcgaggaaa agcgcaaggc cgacgtcgtc cgcgtgcagc gctattacga
     1201 tcagcgcgtc atcgcgcccg acgcggcgtt cctgtggaag gaggccgttg cctgatgccg
     1261 caggccccgg ggtttgcccg ccggaccccg gggcttccct cccgtccgaa agtccaatga
     1321 aaaaggggaa tgcgagatgg cttacgcaac gttgaacgag ctggccgacc ggctggggcc
     1381 ggaactgttg ctgctggtgg ccgacgacga cggcgacggc gccgcggatg aaccggtcat
     1441 cgaggcggcg ctgaccgacg cggcgggcga gatcgatcgc gcgctcgccg ggcgctacgc
     1501 cacgcccgtg gccgatccgc ccgacctgct ccgccgctgg tgcatcgacc tggcgatcga
     1561 acggctcttt gtgcgccacc gcgacgccct gccggccgag catgccgcgc tggcggcgct
     1621 ggcgcggcgg gcgctggcgg ccatcgccga cggcctcgcc ggcctggccg gggcgcgccc
     1681 gctcgcgcgc gaccatgccg ccgccggcac gcacggcggc gaggggcggg tcttcgatgc
     1741 cgaatcgctc gccgatttct aaccccgaaa ggaacaggcc atgaccgccg ggattttcac
     1801 gaccggccgc gcggcggaaa cccttctgcc ggcggccgtg gaaccgggct cgattcacat
     1861 cgcggtggcc gcgcacgaat tcgcgcccgg cgacctgctg ttcgccgccg atgacgccgg
     1921 gaaccacgcc cagtggctcg ggcgggtttc cacccgcggg ccggattccc tgaacgtcac
     1981 gcggccgccg tcgctcgcgc tcgcgccggg gatggtcctc ttccggcccc gcgccggctt
     2041 tctgctggat caggggtcgg tccagctcga ttcgatcgcc gtcgatccgg gaatcgctgt
     2101 ggaattgacc cgcgatggcg caccgcacgc ggccgtcctg gccgagccgg cgactcgggt
     2161 ccgcctggtc ctcgccggcg tgtcgccccg cagggccgcc gatttcgtcc tctggctggg
     2221 cgagcacgcc ggctgggggc tttccgcgtt cacgctggtc ggcgccgacg cgcagcccgt
     2281 cgcgctgcag ctttccggcg agccgctgga gcaatcggcg cgcggcggca gccgctgccg
     2341 gctggcgctg ccggcgatcc tgctcgcgaa ggatgccgtg cgatgagacc cgccgcgcct
     2401 cttgtagttg aacgcagcgc gcacccacgc cagcgcccgg tctggctcct ggaaatcgaa
     2461 acgcccggcg gggcgtggcg cggcgccagc cggccggtgt cggtcggcgc cgcgccgtgg
     2521 ccctgccgcc tgggccgggt tcagttccgc gagcgactgc ccgaaaccgg cccgccgggc
     2581 gggtggattg cgactgatgc ggaagtggac gtgatcgaga ccccgaccac ggccggcggg
     2641 ctgcgcgcgg cggcggcgcc ggcggcgctc cttggggcgc gggccgacct cgcgctggcg
     2701 tggctcgacc cagaggcgcc gccggacttc agcctcaatc cgggcgatcg cgtcacgctg
     2761 ctgcggggcg tcgtcgtcga atttcaagtc cagcccgcca gcctccgcgt gcggctcgcc
     2821 gatccgctgc acgcgcgggc gagccgaccc gtgggccgcc tgctgcacgc cgcgctggcg
     2881 gggggcgagg gccccgccgt cggtcgcccg ctgccgtgga tcttcggccg ggcaagccgg
     2941 gcggaactgc tgccgctgcg gaccggcgtc gccacgcgcc tggcggccga cctggccgcc
     3001 gacgccacga tcgtcgccgt cgagtcgctc gatggtttcc cgcccgcggg ctacgcccag
     3061 gtgggcggcg aactgattct ctatcacgcg gtctcgaccg gcggccaaac gctgggccac
     3121 gccggatcgc ccgtcacccg cgagggggtg catgcctgga ccgccggaac cgaagtgcgg
     3181 ctgcgcccgc ccgaaggctt cgtcttcctg gccgccgacc atccctgcca tgcggtcacg
     3241 atggtgttcg gcgacgaccg gccggcgccg gccggcgact ggaccgccga cgactgggac
     3301 ctgggcggcg tcgcggcgca ggccgtgcgg ctctcgcgct ggccgactga tccggcgggg
     3361 tggtttgctc gccggatctg cgccgccgtc gaggggctgg aacttcccga cggaaccctg
     3421 atggaaaatc ccgccgacgt gatccagtgc ctgctgaccc atccccggct gctggggttg
     3481 gacgcgagcc ggctcgacct ggcgagtttt gccggcgagc aggcgcgcct ggcggcggcg
     3541 gggtatcggt atgcccgccg gctgaccggc gccgagacgc tgggcgcgct gatcgcccag
     3601 gccgcggccg aatccgcctt gttggtcggc ggcgagccga tccggctgct gcggctggag
     3661 ccgacgtccc gcaccgaaga cgccgaactg catctccatg acgcctcggt cgtcgacccg
     3721 cgcggccgac ggggccgggc gacgagtcac ctgcggatcg cgccggcggg cgacgcgggc
     3781 ctgacgccga cggtgatcga gctgatcggg aaggcggcgg acggcctgca accggccagc
     3841 ttctcgctgc cggccggcgc cgccccggcc ggggcaatcc cgaagcggat ttctttccat
     3901 tggctttcgt ttgaagcggc cggcgccgtg gcggaagtcg ccgaggtcat cggcgcgcgg
     3961 ctcgccgcgc cgcgaatcgc catcgaccgg atcgccgcgc cccgcgcgct gctgagctcg
     4021 accggcgccg ccgtccttgt caccgacgcg gcgctcggcc tggatgcgct ggtcggctgg
     4081 atcgaggatg tgacctgcgt cgactttgag gtttacgtgc gcatccacgc ggtcggaacg
     4141 gccgcggcga cctgaaacgc atagccgccg tctgcaatta cacccggaag gagaacgagc
     4201 atgggctggc tggaggaatt ccgcgcggag accgccccgg tcattgccga gccgttcttt
     4261 cgctgtctgg ccgaggcgat cgcggtggtc gaatcggcgc atggcgaaca gatcatcgcc
     4321 gggcccgagg gcttcaatga aatcggctac aaggcgctgg ccaatcatcc ccacgccgtg
     4381 cgcgaaacgc tggaggcggg tccgcgcggc gaactgaaac gcgcccaggc gagctttcgc
     4441 ctcttccgca accggcgcga gcaggcgcag gcgctgctct ggctgctgcg ctcctcgcag
     4501 ttttacgaaa gcgcccggct gctgttcatc ctctgtttct acgcggccta cgcgccgggc
     4561 cggcgggctg gggcggtcgc gctgttgcgc gtcttcaacg agatcgccgc cggcggggcg
     4621 catcgcggcg tgcggccgtt cacgctgctg acgccggaga tagacgcggg agcgcgcgcg
     4681 ctgaaccacg ccgccgcccg ccgcgccgtg cgcctgttcg gcgagctcac cgccgcccgc
     4741 cgcgtcgacg actgatggga aggggaccgg ccgtggacga ccagctcctg cgccagctta
     4801 accagcggct cgacgagcac cacgcacacc tgcgcgagcg gctcgacgac ctgcgccgcg
     4861 agatgcagac gctctacggc acgatcgtcg cccggctcga tgcgcacgaa acttaccatc
     4921 gccagtgtga gcatcgctgg ggcctgccgc ggctggcgca gcgccatccg ttccggctcg
     4981 cgctgatcgc gttcagcgtc gcgtggctca tggccgcggc cgcgcccgaa tcgctgcaat
     5041 ggctcacgcg gctggcgctg gactggctcc ggtcgctgcg ggggtgaggg aacctggtcg
     5101 ctgccgacgc ccggccgcgg attcaaaatc cgtcggatcc gtcggatccg atcgatccga
     5161 cggatgacga gcacgaggcg atcacgagga tgaggacgag ggacgaacag ggtttgttcg
     5221 ccagcagggc tcacctgacg ggcgtatagc ccccctccac gccgcagggc gagtagacga
     5281 tctgccacag ctgattgacg cgcgcgcgga agccgccggc gcagctcttc agataaaatt
     5341 cccacatgcg gtggaagcgg tcgccatact gctcgcggaa gcgcggccaa ttatcattga
     5401 aattgtcgaa ccacgccatc agcgtcctgt cgtaatacgg gccgaagtta tgccagtcct
     5461 ccatcacgaa cagcccctcg gcggccgtcg cgatctgccg cgccgagggc agcatcgcat
     5521 tggggaagat gtagcggtcg atccacggat cgatgcagct ttccgagcga ttgccaccga
     5581 tcgtatggag cagcagcagc ccttcgcgct tgaggcagcg gcgggcgacc tcgaaaaaca
     5641 accggtagtt gcgatgcccg acgtgctcga acatgcccac cgaaacgatt ttgtcgaacg
     5701 gttcacgcag gtcgcgatag tcctgcagcc ggatctcgac gggcagcccg gcgcagcgtg
     5761 cgcgcgccag ctcggcctgg cgttccgaga tcgtcacgcc gacggcctcc acgccgaacc
     5821 gctcggcggc gaagcgcagc agcccgcccc agccgcagcc gatgtccagc agccgttcgc
     5881 ccgccttgag cgccagcttg cggccgatca gctcgagctt ggcctcctgc gccgccgcca
     5941 ggttttccgc ctccttccag tagccgcaac tgtactgcat gtacgggtcg agcatggcgg
     6001 cataaaaatc attgcccagg tcgtaatggc gccgggcgac ctcgcgcgac cgcgtcctgg
     6061 tctggcggtt cgcgacgcgg gcccagagga ccgtcgccgc gttgcgcagg ttgcgctgca
     6121 cgcggcgcga gagatcgagc cgcaggatcc gcgcgatcaa ttcgtccagc gccgcgcagt
     6181 cccaccagcg gtccatgtag gattcgccca ggccgatgga gccctcggcc aggacgcggt
     6241 tgaagaatcg cgggtcgtga acctggatgt cccacggccg gccgccgtcg atgcggacgt
     6301 cggcccgctc cagaatcgca tcgatgagcg cgcgcccgcg cgggatgcgc cccgcggggg
     6361 cctgcgtcaa ttcgcctgtc agcatggtct ctccgcctcc tgcaactgat accgatcggg
     6421 ggcagccggg atcctcgcca aagggatcgg aggaagaagg cgcgccgccg gcgagcgcgt
     6481 cgccgggaag agctcgactc cgcccgatca agagaggctc caattatcag ccgccgccgc
     6541 ccgactctgc aaacaaaaga cgagccagcc aaaccggcga acgacaatca gcttacccac
     6601 ccgggtaatg agagaaacag gccgtaagat ggagatatgc gaaaatggta cggaggccgg
     6661 cgccgtgcag gtcatgggct cacccggcgc gggccaggcc ggcggaaacg caaagggctg
     6721 cggctttcgc tattccgcgt tcagcacttg ctcgtagcgc gcgcggaagc gccgctgcca
     6781 gtcgatttca agctggctcg cttcgcgcgg gctcgcctgg tagcgccgcg ccaggtccag
     6841 cgcctcttcc tccgaaaaag ggacggcgtt gaattgccgc cgcagcggcg cgtcggccgg
     6901 cagcgtcatc accttgcgcc aggcctggcg cagcagctcg ctgttgcgca ggaacgccgc
     6961 catgaacagc ggaccgagca gcggcgagcg gcgcgtggag agatccatgt cgaggttgaa
     7021 cgtcgtctcc gaggcatacg gattgaagtc gatcagccgc tggtcggcgg gatgctcata
     7081 cgccgccggc agaatcggca tccgccagag cccgtgctcg gcgggtccgc ccggcgtgcc
     7141 gggcttgagc agccagagcg actggcccgg cttggtcagg acaaactcca tgaatgcctc
     7201 cgcggcggcc gggttgggtc cgccgcgcag catgcagatc gggtcgggcg tcagcgccgt
     7261 ggcgttgacc gggctgacat agcccagcat ctccgcgccg gtcgattcga tctggctgaa
     7321 ggcatagaaa tcgatcgaca tgccggcggc ggcgctgccc tgcgccactt cgctcggcac
     7381 attggaggcg ctgtcggtga attcgcgcgt gttcccggcg atttccatca gcagggccca
     7441 gccttccgcc cagccgtgtt tctgcagaat gacttcgaac gccacccgcg cgctgcccga
     7501 cttggaggga tcggccagca cgatgtggcc gaacagcgcc gggttgctca gatccgacca
     7561 cgtcgccggc gccgcgacgc ccagcgtccg cagcacctcc ttgttgtaga tgatcccgaa
     7621 gctggagagg acggcgccgt accaatggcc gtcgggatca tacagccgca cgccggcgat
     7681 ctcggccggc aggctgtcga gcaccggctg ggggagcgcg gcggcggtcg tcgcgccggc
     7741 ggccttgagc tgctccatcg cctcgatgcc gcccccccag accaggtcga cgcccatccc
     7801 gtcgtccttg tcgtcgaggg cctgataggc gccggcgatg gcctcgacca tctgatacgt
     7861 cccgccgcga tcctgccatt cgaattcgac ggccggcgcg ccgccgcggc gctcggcgta
     7921 ccagcgctgg aagccgcgtt caaactcctg gcggatgaac tcgttgtgcg gcgagacgat
     7981 caccaggcgg gtcgctgctg ccgtcggccg gggcgtagcc gtgccgcccg tgggcgccgc
     8041 cgcagtcgat gttcccggct cggttcgggg gccgcagccc aggaggatca gggccggcag
     8101 gagcgccgcc agggcgatcc agcggccggc gcgaaaggcg gctccggcgc gtcggcgccg
     8161 agcgtctgtc acaggcttgg aaatcggcat ggggacccct ttccgcgggg ggaatgccgg
     8221 cgattccccc gattgttccg ccgcggcggc gcggcgtcaa tcttcagccg gcaggagcat
     8281 gacatgctcc ggcgcgacgt gccagcggac gcgggcgcct tccgcgagga cttcttcccc
     8341 cgcattgagt tcgagcgccg ccagcgcccg cggctcgccg ccctcggtcg ccaggtcata
     8401 ctgcgtcacc tcgccgaggt aggacacagc agtcacccgc gcctcgaaga cattgacctc
     8461 ggccgcgtgg cggcggctca gcgcctcggg gcggatgccg agcagcacct cgcccgcggg
     8521 cggcgcgccg tcggcgcggg cgtgcagcgg cccgaccggc gtcgcgacga ccacgcgatc
     8581 accggccgcc gcgcccgtca cccgcccgcg cagaaagttg gtgtgaccca gaaaatccgc
     8641 cacgaaccgg ttgaccggat ggtgatacag ccggcgcggg gggccgagct gttcgatctg
     8701 tcccgcgcgc atcaccgcga tgcggtcggc gatcgccagc gcctccttct ggtcgtgcgt
     8761 gacgtaaatc gccgtcagcc cgctcgcctt gcagatgcgg cggatctcgc cgcgcatctc
     8821 ggcgcgcagc ttcgcatcca ggttcgacag cggttcatca agcagcaggc aggccggccg
     8881 gatcaccagc gcacgggcca gcgccacgcg ctgctgctgg ccgcccgaaa gctggttcgg
     8941 tttcttcccg gcctggtcgg cgatgcgcac gaccgccagg gcctcgtcga cgcgcgcgtc
     9001 gcgttcggcg cgcggcaacg cttgcaaccg gagcccgaac gccacgttct cgcgcacggt
     9061 cagatgcggc cacagcgcgt aggactggaa gaccatgccg gtgttgcgcg cctggggggg
     9121 catgcgcgtg gcgtcgcgcc cgccgatttc gatccgcccc gcgtcgggcg ggacgaagcc
     9181 ggcgatcgcg cgcagcagcg tcgtcttgcc gcaacccgac ggccccagta ggaagaacag
     9241 ctcccccgcc tcgaccgcaa gcgagacgcc gtccagcgcc cgcagcgcgc catactgctt
     9301 gacgaggttt tcgacgcgaa ccggctgcac cgcgcacctc cgcgcgccat tccagcagat
     9361 gcccgcgccg ttggaaagga aggtttttat gaaccggggg gttcatgttt cgagggccga
     9421 ggacgaggga cgagggacga gagccgagag ccgaatttgg aaaaggtctt tagggagggc
     9481 gagggacaag ctccgtattc aacacgaaat ccgtcggatc cgaccgatcc gacggatttc
     9541 catgaggcaa tcgttaacga accggccgtc gcgaggcgga ggcggcgcgc gcgttcagcc
     9601 gcccagcagt tcgttcacct tccgcatcac ggcgaaggca tcggcgacat acagcacatc
     9661 ggccttgccg cgcgcccacc cgcagttcgg gtccaggttg atcgcccggc gctcgttgat
     9721 gaaacgccag ccgacgacgt gcggctcctc gccgtggcag caggtggcca agcccttcgg
     9781 gtggcggggg gtcgcgccgg tctggccgac ctggttcagg tgcgtcagga acgggaggac
     9841 cggttgtccc tcgccgccga cgtcgacgag cgacttgctg ccgccgagcg acgcctgcgt
     9901 ccggtcgaga aagccgagaa tcagttgcgc cgcttgctcc acgtgcgact ggccgtcgcg
     9961 ctggggcttc gtccagccgg cgccggcgac gaacagcaac tcggcatcgg ggcggacggt
    10021 ctggccggcg ccgggcggcg cctggacgcc ctcgacgcgg gtccgctgga gcgactcggg
    10081 caggtcgacc gcgatccgtt cgatgcgcgt gggcgccgcg tcgccgtcaa atgccggcat
    10141 cgtgcccggc gcgaccgtca gcagccaggg acgcgtcgtc cgtgtcagcg tcgcctcgat
    10201 ccgctggcga tagaaccagc gatcgacgac aagttggccg tcgtggactg cgagaccggt
    10261 gatctgtgta tcgacgcggc cgcccagacg ctgggccaga ccggcgatga accggctgac
    10321 gcggctgacg gcgggcgcga agacgagcgt cggccgcgcg gcgcgggcaa tcgcctcggc
    10381 cgcggcgctg tcggttgcgt agcgcgaaat gccgaacgcg ctcccctcga cgagatacag
    10441 tgcggcgcaa ccggcggcgg cggcggtccg gctgcccggc tcgcagtgct cgccgatgag
    10501 cccggcgcaa aactccgcgc cgagctccgc ggcggcctgg cgcgcaatcg ccagcgtttc
    10561 acgcgccgtc tttcccagcg acccatcggg ctcgatgcag gccagaaaca aaacgttttc
    10621 cattacttat accctttttc caatacaatt tcatccaggt tcgaggcgga tggctccgcc
    10681 cgactcaacc acgaatccag gcaacaattt cgcgagcaat ttcgtcgacc ggcatgtctt
    10741 tcacgatgcg cgtttcacgg cgccggcgcg gcgcctgcgc gcagcaatac ctcaggccgt
    10801 ccatgccgag ttgcgcgggc cgggccttct gcagggcggg catgacgccg cgcatgtttt
    10861 gcatcccgat ctgcgggttg ttcggcggtt cggggcgatt gcccgtggcc cagccgagca
    10921 ggacgggcgg gccggcgcag agcgaaacct gattcagtcc gccgtcggcg cgttcgacaa
    10981 cgcgcagcgc gccgtcgggc tggaccgcga gctcatcgac gccctgaaac tggtcgacga
    11041 tcccgaggtg ttcgcccacc atctgcagca cggcgccggc gccgcggctc gccgacgccc
    11101 agccgccgca caagaccagc cgggcgcgat ccagcccgtc gatggccgcg atggcgtcgg
    11161 cgagcgcttc ggccgttgcc gccgcgtcca cgaaacctcc ggccggaccg tccagtgcca
    11221 ccagttgcag cacgaccttc tggccgatcg tcatcatcac ctgctgcagt tttgcccggg
    11281 ggccgatgct gaccagccac aggcggctgc cgggaaccga ctggaccagg tgcgccgcct
    11341 cataaagcgc gtgcgcggcc cacgggtcga acaccgccgg caggatcatt tcgtttttca
    11401 attccgggcc gttgggtccc gtcaccggct ccaggggatg caacggatcg ggaacaacgc
    11461 tcccgcagac gacgacatgg tatccactca cgaataagtc tcctgttaag aatctggatc
    11521 attaaaaaca cgaaaaaact cgaaatctgc attcattgaa cgatgcggcg atgcgtcata
    11581 atggactcgg gtcagcgccc tgcaacggcg caacggcaca ttgcctttga gtattaaaaa
    11641 tggaattttc ccgttttctt tcgcgctttt cgtatctttc gtgatccctg cttaattttc
    11701 cgcgctgtgc aggccgccgg cgccggcgcg aaagaccagc aaaccgcggt gcggatcctc
    11761 ggggaaggat tgcgaacagt tccacaggca ggcgccgcag tggacgcatt tctcgcggtc
    11821 aaacagcggc gccccgccgc cggggttggg cgtgatcgcc tggcccgagc agatttccac
    11881 gcagaccggt gcgccgcact ccgcgcacag ttccggaaaa ggaaacacga cgtgatccgc
    11941 atagccggcc ggcgcctgca ccttgccgcc gagcagcagc gcgtcctggt gcgagacgag
    12001 gagccgcccg tcatattcca ccggcggcca gccggcggcg tccatcaagg cgtcgtgcag
    12061 cggccggccc gacgcgcggc actcgcgccg gatgcgggcg atccggtcgg gcgggatgcg
    12121 gccggcgtgg aattcctcca gcgcggcaat cccctcgtgc ggcggcacgg accgcccggg
    12181 ccaggccagc cggccgcggg tcagcccggt cagcgccatg ccgagcaagc cgggaatgaa
    12241 tccgcgctgg aagccgtcgc gcgcgcgttc ggcgatgcgc gattcttcgt cgacccagct
    12301 tcgccgccgg cgcgcgacat acgccgcttc cagattggcg cgcgtgcact ccttgcccgc
    12361 ccgccacagc tcggcgacgg cctcggccag ttggcagccg gtggcccatg cctcatcgac
    12421 gcccgagccg gtgagcacgt tggtcgaccc ggagccctcg ccgatccggg cgaagccgtc
    12481 gcccgcgagc cacggctcgc cgcgccggcc cgattcctgc aagcttttcg ccccccacga
    12541 gcgcagccgg cccccctcca gatgccgcca gaggtacggg tggcgcatcc agtgctgcag
    12601 gtagcggtag gatgtccgca ccggactgtc gaaccacgag gggacaaaga tccccagcga
    12661 cgcgacccgg tccggcataa catagaggaa cccgaacaat tcgggttccg gatagccgag
    12721 cgtgtggatc accgtccccg gttcgagcgc gcaggactcc ggcaggtcga ccaccatttt
    12781 catcccgacg gcccagtcgt tccgctcatg gtccggcggc aggcccagtt cgcggtcgag
    12841 ttgctggccg accgcgccga ccggcccgtc gccgacgacc gtcagccgcg agacaatatc
    12901 catgcccggc aggtagcccg gctcgggccg gccgcgccga tcgaccccct gatcgatcag
    12961 ccgcacgcca tggacccgcc cctcggcggt cagcgccgcg gcgaccggcg tcgccggcca
    13021 gatctggacc gcgcccgtcc ccatcaggcg ggcgccgacc cactggttga actgccccag
    13081 cgacatgacg aacccatcgt ccttgcgcag gaagggggga atataaggca gctcgaacgc
    13141 ccagtcgcgg cgcacccgcg gcagccgccg cagcgcgcga tcggccaggc gcatcaatcg
    13201 cggccggcgg ctcgcgccgt gcggatcgag caggtagacc agtttttcgt tcgtcaccgg
    13261 cgccgccatg ccgagctggt tcaggtccag gtcgggcagg ctggcgcgga tggcgcggcc
    13321 gcgcgtcgcg acgcccgaaa cgccgaagcc gatgtcgtcg gcgcgctcgt agcagaccac
    13381 ctgcagcggc aggccgggca tcgcgcggct ctccagcgcc gccgccgtcc gcgggtcgct
    13441 cagcttgttc gccagcgtcg ttagaaatcc ccccatcgcc gggccgaagc cgacgcaaac
    13501 gatgtcgacc tccatcgtct ggcgttcgac ggcgggggtt gggctggcca aagcgttcat
    13561 gggtgcattc cctcaatccg cgtcattcgt ccttcgtgct cgtgatcgta cgaaaattca
    13621 tgtttcggga gggagggcat tttcatcctt tcagggggac gcagaaaggc gtcgtgagaa
    13681 gctcactcca aaatcgtcgc ttcgtcctcg tgatcgcccc ccaaggcccg ttccgtcccc
    13741 ctaccgcgga taatccaggg cctcggggat catgatccgc gccaggttgt gcgccaggcg
    13801 gtccttggcc aggcgcgaac cggtcaggca gccgtccatc cgcaggcgca gccgcaggaa
    13861 tcggttcatg ccgtcgaagc ggacgcacgg cccggccttc ggcgggtgga cctcgccttt
    13921 caccttcacg tcggcatacg cgacggccgt cccgccgatc cccggcatga cgctctccat
    13981 ttcgtccagc tcgcccatgt cgtaacaggc atcgcagctt gcggcgtccc acgccggatg
    14041 ggcgttgtag ccatagacca gttcggcgca gatgcggccg acttcgccgc aggcgcgcgc
    14101 cgcctggaca tggcacaggt cggtgtagaa tcgcgcgatc ccggcggcgt tctcggccag
    14161 caccggattc tccccggccc tggccttcag ctcctccacg tccaggatca ggctgcgcgt
    14221 ggccagcagc cagcacagcg cgtcggccag cgggaaggtg accccctggc gccggctttg
    14281 atagagcaac tgcccctcgg cgtccctggc ccggcgcagg tgattgagcg tccacagcca
    14341 catctgcatg gccgtggcca ggccacaggc gccggaatcg ggcgtctgcg agccgatccg
    14401 gcgcagctcg ccgatccact ggcggaactg ggccaggaag acctcgttcg tcatcgtgac
    14461 ggtcaactgg cggcgctgga ccgcctccgg cccttcgtag gtcgcctcaa gctgggcgtc
    14521 catccacttc tggccgagga agccggggca atcctccgtg atgccgtagc cgcccatcag
    14581 gctgacggcc tcgcgcatca tgttggcgcc gtggccggtg ttccacagtt tggccgcggg
    14641 gcagagcacg ttggtcagcg cgtcgagccg ggcgaactgg acgagttcgt cctgctggag
    14701 ctggtcgaac cgggcggcgt cgcgttcggc ctccggcagc ccttccagct cgatcagctc
    14761 cagcgccgct tcattgagcc ggcgcatctc cttgagctgg tggcgcccgg tcaggccccg
    14821 ggccgcgaag agctccagct tgcgcgcctc cagcggatcg aactggtcga acaggcgcgc
    14881 ggcctcgaag ccaagcgacg cggccgattc ccccgtcgcc cagacatcga tcagccggtg
    14941 cagcgcgtct tccttctgtt gcagacccat ctcgtggcgg accgatccgg gctcgccggc
    15001 gtcgccgccg cggaatcgcg tccggtggta gcggatgatc ggttcgatcg ccgagagcag
    15061 cttggccgcc gtcatcagcg ccaccgtgac gcgcgtccgg cggaagaccg cctcgatcac
    15121 ctcgccgtgg gtgtagttgg gaatgaggac tccgtcgcgc accgtgtaac cgccgatgat
    15181 ccggctggcc ggcacgcgca ggttgagaat ggggtcgcgc gtcgaggaga gctggtggac
    15241 gagcttgcgc gtcgggaggc cgcggtcgaa caggccgggg tcggtctcct ccagaatcac
    15301 catgcagctc cccttgatgc gcgggtcgtc cgactcgacc gccgcggtga cgaacgtggc
    15361 gaaacccatg ttggtgatga accggccgcg cttttcgact tcaagggttg gctccacatc
    15421 cgccggccag tcatggatcc gcatctttcc gcccaggacg cccgtatcga cgccgacaaa
    15481 cgggatcggc tcggtcagcg cgaacgcgcc gcgctggcgc cgcgccgcgc ccggcacgac
    15541 ggcgctcatg taggccgctt tctgttccgg cgtgccgcgc tggtggatcg gcgcgagcgc
    15601 caggtcgccg gccaggctgc atgtcgccgc gccggcatcg acccacgcca gttcgaacgc
    15661 cagcatcgcc agcacgaagt ttttcggccc ggccaggtag ccgccgaatt ccggatcgat
    15721 gccggcggcg gtgatgccgg cgtcgtcgaa cgccgacagc aggccgcgct tggcctcggt
    15781 ccactcgtgc gtgttgcggg ccccttcggc cacgagtcgc gccaccggcc cgcgggccac
    15841 cgcccgcgtc gcctggatca gcatctggta gtcgaatcga tcgctgaagc gccacatgag
    15901 ctggcggatg tcgtcgccgg gcagagtgag catctgatcc atggtgaggg tccttcggag
    15961 agggtagcgc cggggaagtg gttgaggccg ccttgggggg acgcagcgcg ggggagagca
    16021 agcggccggt cgaacgcgcg ccgccaccgc cacgcgccgg ttgagcgagg cgaagtgact
    16081 tgagcccgca ccgtcgccgg gcgaccatta gagaacgaaa cgcggcgcaa ggcaaggaat
    16141 attaacatat tatgatcctc atatctttta ttcgacgggg agatacgctt ggaattcgag
    16201 gccgaatgga aaattccggg ggcttcgtca gccgaggacg agcgacgaaa accggatttt
    16261 ctggcaaacc gattctccgc gtctcggcgt gagcttcttg ttccggcgag cgagtctgtc
    16321 tgtgaagccg gcttcttctc cggacggaat cagttctcac gcagagacgc ggagacgccg
    16381 aggacagacc gggtgcaggg ggtcagcagt gagctggggg ccaccacggg aatagaagaa
    16441 gatagaggga tttcgagagt cgaggacgag cgacgagagc cgaattctgc ggggggccgc
    16501 tttgggcgag gagcaggacg agagacgagg gacgaattcg ggggaggtca gggggaggtt
    16561 ggcgcggggg ggcgggtttc gagctgggcc tcgaccagac cggcccggcg gcagatgtca
    16621 aagacctcga tcacgcggcc gaggttgacg gccgtgtcgc cgcggacgag gacggcgcgc
    16681 tgcccctcgc cggccagcgc gcgcatttcg agctcgagtt cctcctccac gacgcggcgg
    16741 tcgccgtaga agatgtcgcc ggccgcgctg accgccacgt gcgggatcgt ctcgccggcg
    16801 gccgcggtga ccgtcgagga ctcgggcaac tgaatcgaca tttcgaaggt ttcctcgcga
    16861 atggtcgtcg cgaggatgaa aaagaaaatc agattgaaga tgacgtcgag catcggcgtg
    16921 aggttgacgc cggcctgcag gcggcggcgg cggtggatcg ggacgcgcgc gctcaccggc
    16981 ccgcctcatc gggtcggggc gcggcggggg ccggacccgt cgccggttgc gccggggccg
    17041 ccggtcgcgc gaccggccgg gcctgtccgg gcaccgcggc ggggcgggcc gcaatgccgc
    17101 cgggctgcgc ggcgggacgg gccgcggcgg gggagggcgc gcctggccgc gttgccggtc
    17161 gcggctcggc ggcgggccga gcgggagctc gcggctcggg cggcgatact accggacgcg
    17221 cggggccggc cgcaaccacc ggcgcgggaa ccggtcgcgg cgtcgcggcg ggctcttcct
    17281 cgtcgcgcgg ggggccgtag atcgcttcga gcacgcggga gaccgcttcg ggcagccggc
    17341 gccgttcgta gtcggcgatg atccgttcga gccactgcgc cgcgacatag gccggaatgg
    17401 cgatacccag accccagagg gtggtcacca gcgcctcctg gatgccgatc gagaggagct
    17461 tgaccgagcg ctcggtcgac tgcgcgaaga catagaacgt gttcatcatg cccagaatcg
    17521 tcccgagcag accgagcagc ggcgcgatgg tgtagatcgt ggcgaggagg gcgaggccgt
    17581 cgcgcatctc gtcgccgact tcggccgacg cctcttccat tgaaatcagc agttcctgct
    17641 gcggcggccg gcgcgtctgc gcgacgttgt gcagccgcag ggtcagccag acggcgcggg
    17701 ccagcggcgc gcgcgactcg gacaggccga tcacgaagcg gcggcgcatg tcgggcgact
    17761 tgaccgactc ggcctggcgc gtgacccacc ccggcagcac gcgccccgac cggagccgga
    17821 tcagcccctg gatcaccgcg cccagcatca gcagcgaaca cgggacgagc aggaacatca
    17881 ccggcccgcc gttgatcacg tatttgatca gcagtgtttc catggggttg aaagcgtggg
    17941 cgtgggcaag ggtagcgggc ggcgcgcgtt tcgagccgcg ccagtatcat acgagccgcg
    18001 ccggacgatg accagcgcgg agtggggaga actgcggccc acttccatta acccaccacg
    18061 gccaccgctc agcgttcggc gagggggaga tcgaccatga agacggtgcc ctgctcgggg
    18121 gcggggcgga ccgagatcga gccgccgctg ttctcgatga tgcgttgcgt gacggcgagg
    18181 ccgaggccgg tgccgtgggc cttgcgggtg aagaacggct cgaagatgtt gtccagatcg
    18241 tcctcgggga tgccgatgcc gttatcctcg atttcgatcc gggcgcggcc ggcgcgcggc
    18301 tggttcagga tgcggatgac gatccggccg acgcgccctt ccggaatcgc ctgccgggcg
    18361 ttgaggtaca gattgagcag ggcctgtttc aactgattgg ggtcgacgct gacgatggtc
    18421 ggctcgggcg tgtattccgt gacgatgagg agcttctcgg tctggcggcg cgggaagacg
    18481 aagcgcagga cttcctcgtt gagctccttg aggtcgagcg gcgtcgtccc gctcggccgg
    18541 ggattggcgt aggtcagcat ttcctggacg atgcgtttgc agcgcatcgc ctcgcggtgg
    18601 atcgcctcga tctcctcggc cgcctcgctg tcggcgggca ggacgcgttt gagatagtcg
    18661 gtggtggcgg cgatgatgcc gatcgggttg ttgatctcgt gcgcgacgcc ggcggccagt
    18721 tcgccgacgc gggccaggtt gtcggcgcgc agcagccggt cgttcgcctc gagcaattcg
    18781 gtcttctggc gcgtgatgat catcacgatg aaccacgaca tgaggatgac gagccagatc
    18841 aggaccagcg agacggcgaa attcgcgtcc agatcgtagc cggggacgta aaacgtaatg
    18901 aaatagagaa tcgagatcag caggttgacg aaaatcgttt cggcgatcgt cttgaacagc
    18961 gcgaagccgc gcatgaccag caggaaatag agcacgtaga aatcatggtg cgtttcgacg
    19021 ccgaagctgg tcgtcgccag gtccagatag atcagcatgc tgacaaagac gacgtcgacc
    19081 agatacgaga agaggaccag cggccggatc tgatgattgg cgacttcgct gaaatagaag
    19141 aagtaggtct gcgcggagtt gaacaggaag tagaggacga agagcatgaa gacgatcgtg
    19201 ctgggcgggc gcttgatgat ccacacccag aggaccagcg tgacgacgag gatcacccat
    19261 ttgaacggga taatggcggt ccgttcgagt ttgtagagcc cctggcggta ctctttctcg
    19321 cggacgggga gcggtttcat gggcggggat caccggctcg cggcgggaga gcggcccggg
    19381 agccgagccg gccctggatg cgtcggatcg cctgaacgat cgcctcgggg gcgggcgact
    19441 cggattggac ggtcggcggg ggctcctcca gctcggcggt gcggcgcagc aggcgggcgt
    19501 gcgttgtcgg gccgatggtg atccagccgg cgcgcggcag gcgcgcggcc gcgccgaaaa
    19561 agccgaaaat cgcctcgacg gccgagggcg aggccaccac cagaacgggc ggccgtgcct
    19621 ggcgcgccga ctcgccgagg gcctcgcgca gggcgcttaa cccttcgggg cacggcaatg
    19681 tttcgtacag cacgatggac tcaaccagcc gtccgcgccg gcgcaagccc cggggaactt
    19741 cggggcgcga ctggcgcgag ccgacgatca ggattggctg gcgcggttcg gcgggcaggc
    19801 cggcggcgag ctcggcggcg gtcggcaggg tcggaacgaa gtcggcctcg cggccccagt
    19861 gctcgcgcag ccggcggttg gagccggccc cgacgacggc cagccggagg cccgcgagct
    19921 cgcgcggatc gacgccgcgg cggcgcagcg cctgggcgag tcgtccgatg gccgtcggac
    19981 tggggagaat cagccagccg cgcgcccggt cgagccggcg gatgcggtcg gcgagggcgg
    20041 gatcgtcggc ggccgggcaa tcagcgaatt gaattacggg ggtcaggcgg gagtcgaagc
    20101 cggcggcgcg gattgcatcg ccgagcgccc ggttctcgcc gtcgggacgg gtcacgagca
    20161 caagcgggcc ggcggccggc gcgggggcgg aagccgtgtc ggccggcgag gtcatggcgc
    20221 accggcgggg ggctcggccg ggctgagcgt ggcgcggatt tcgggcgacg cctcggcgag
    20281 catcgaacgc gccagcgcca gcgccaacgg gccgagttcg tcatcgtggt cgcagaccgc
    20341 cccgcgccgc agcagatgac gcccgtcggg cgatgccagc acggcggtga cgcgccagcc
    20401 gcccccctcg cgcggctggg cgaggacgcc ggcgggagtt ttgcagccgc cgccgatggc
    20461 gccgatgaag gcgcgttcgg cctcggcggc cagccgggtc gggatgtgtt ccagttgcgg
    20521 ccagatgtcg gtgaattccc ccgcgcgagc ctcgatgccg agcgcgccct gacccatcgc
    20581 gggcgtgagc tgatcggggg aaaagacgga ggtcaggacg tcgctcaggc cgagccggtg
    20641 cacgccggcc tgggccagca gcgtggcgtc ggcgaggccg tcgcggatct tctggaggcg
    20701 ggtgtggacg ttgccgcgga tcgggaccac ttccaggtcg gggcggaggg ccaggatctg
    20761 ggcctggcgg cgcaggctgc cggtggccac gcgcgcgccg ggcggcagat cggccagcgc
    20821 cgtcccgtgg cggctgaccc agccgtcgag cggcgattca cgctcgggga cggcgaccag
    20881 ttccagaccg ggcggaagtt gcgtgggcag atccttcaga ctgtggaccg ccagatcgat
    20941 tgtgccggcc agcagggcat cctcgatttc cttggtgaag atgcccttgg cattcatccc
    21001 gccgaaggcc gtgcgctggt ccagatcgcc gcgggttttg atcaggatga tctcgacggt
    21061 gcagccgagg gcgcgcaggc gttcggcgat gtaatcggcc tgccagcggg ccagttcgct
    21121 gccgcgggaa ccgatgcgca ggtgcgtttt caaggcgggc ggcgacacgg gcaacctttc
    21181 ggcggcgcgg cgaagtcgtg gcgaacatat ccctcatgat gaaccgaacc ggccaaaaat
    21241 caaggttcgc ctcgcggggg agggggggag cagagggagc gccccgacaa ggtggaacag
    21301 tccctcatgt gacgcgccag ggctcggatt cacaatgagt gggcggaagt gaaagctatc
    21361 gagagccgag gacgagggac gagagccgag agccgaatta ttggaaagct caatagatac
    21421 ccaattccag gcgcgcggcg tcggacatca tggattgatt ccattgcggc tcccagacga
    21481 cgtcgacggt cacgccgcgg atcccctcga cgagggcggc cttctgctcc acttcgccgg
    21541 gcagatagcc ggcggcgggg cagttgggcg cggtcagcgt catccggatg tggcagaagc
    21601 ccgtgggatc gacgtcgatg ttgtagatca ggcccagatc gtagatgttg accgggattt
    21661 cggggtcgta gaccgtccgg atgacctgaa tgatcgcctc gcgcagcgct tcggtgtcgg
    21721 catggtaggg gtcggccggc gccgaggcgg cggccgagtc cggaccgggg gccgggccgg
    21781 tttcgtccgt cggcgtcgcg gcgggcgcgg gggccggcgc ggggggttcg ttttccagat
    21841 cgcgcgccgg ttcgtcctcg ccgcggccga acagcttgct gatgttgaac atgatgcgtt
    21901 actccgtgct gacgatctcg ctgccgcgag tcagtgccga atggaaggta tgccacggca
    21961 gcgtcgcgca cttgacgcgg atcgggaact cgcgcacgcc ctccagcaca tgcagtttgc
    22021 ccagttcgac ttcggggtgg ggcagcttgc cgtcggtcag catgtggtgg acgttctcga
    22081 agaggaactg ggcgcgccga cgcgtcgcgc ccttgagcgc ctgggtcatc agcgaggcgg
    22141 aggcggtgca gatcgcgcag ccgctcccct ggaaggaaat gtcgcggatg gagtcgccgt
    22201 cgaggtcgac gaagaccgaa acgcggtcgc cgcagagcgg gttgttgccg tgggcgatgc
    22261 cggcggcggc ctcgttggac cggaaattcc gcggattgcg cgaatggtcg aggatcatct
    22321 cgcggtagag atcgtccaga tcggccatca gccaaacacc tcgagcacct tgcgcaggcc
    22381 gcggaagagc gcgtcgacct cctcgcgcgt gttgtagaac gagaacgagg cgcgggccgt
    22441 cgccggcacg ttgaaacgct gcatgaccgg ctgcgcgcag tggtgaccgg tgcggatggc
    22501 gaccccttcc tggtccagga tcgttccgat gtcgtgcggg tgcgcgcagt cgaggacgaa
    22561 cgagaggacg ctcgccttgc agggggcggt gccgatgatg cgcaggccgg ggatatcggc
    22621 ggcgcactgg gtggcgtagg ccagcagctc gttctcccag gcggcgatcc ggtccatgcc
    22681 gaggccgagc agataatcga tggcgggggc gagggcgatc cccccctcga tgttgggggt
    22741 gccggcctca aagcgctgcg gcgggtcgtt gtaggtcgtc ttctcgaacg tgacgacgtt
    22801 gatcatgtcg ccgcccccct gccacggggg catggcctcc aggtgcgcca gtttgccgta
    22861 gagcacgccg atgccggtcg ggccgcacat cttgtggccg gagaaggcgt agaagtcgca
    22921 atcgagctgc tggacgttga cccgcagatg cggggcggcc tgcgcgccgt cgatcagaat
    22981 cgggatgccc gcggcatgcg ccagttcgat catccggcgg atcgggttga tcgtccccag
    23041 cgcgttggag acatggacgg ccgccacgag gcgggtccgc ggcgtgagca ggcgggcgaa
    23101 ttcctccatg cgcaggatgc cctcgtcgct gatcggcgcg acgcgcagcc gcgctcccgt
    23161 ctgctcgcag acgagctgcc aggggacgat gttggcgtga tgctccatgg cggtgatcag
    23221 gatttcgtcg ccgggctgga gccgcggccg ggcgaagctg ttggcgacca gattgatcga
    23281 ctcggtcgtt ccgcgcgtga agacgatctc gcgccggtcg gcggcgccga tgaactggcg
    23341 caccttgtcg cggctgttct cgtaggcttc ggtcgccacc tggctgagcg tgtggacgcc
    23401 gcggtggatg ttggcgtaat cctgctcgta gagcttgcgg atcgcctcga tgacgacgcg
    23461 cggccgctgg ctgctggcgg cgttgtcgag gtagaccagc ggcttgccgc ggatcttgcg
    23521 ctgcaggatc gggaagtcct ggcgcacgcg ctcgacatcg agcggctcga cgcggggggc
    23581 atgcgtgtcg cgcaaggcgt ccaggggaac ggctgagctc atgcggcgac acctccctgc
    23641 tcttcgccgc ggaaccggcg gatgatctcg cgttcgagcc gatgatggat cggctcgatc
    23701 cggatctgct cgatgatctc gttggcgaag gcgtaggtga gcaagccgcg cgccgcctga
    23761 aggtcgatgc cgcgcgtcgt cagatagaag agcgcctcgg gatccaactg gccgatcgtg
    23821 gcgccgtgcg tgcagcggac gtcgtcggcg aagatttcga gctgcggctg cgtattgaga
    23881 ctggcctgcg gcgacagcag cagcccccgg ttctgctgaa aagcgtcggt cttctgcgcg
    23941 tcctcgcgca cgaggatgcg gccggtaaag acggcgctgg cccggtcgtc gaggatgccc
    24001 ttgtagagct gggtgctgcg gcagttgggc cgggcgtggt cgaaccgggt gtggttgtcg
    24061 aggtgctgcg cgccatccag gatgtagagg ccgttgagcg tcgattcgat gccctcgcca
    24121 tccagaacgg gattgatttc gttgcggatc aggccgccgc cgatcgaaat cgtgtgggtg
    24181 gcgaaggtgc tggcgcgcgc ctggtggaca tgatgcgtcg cgacgtgaat cgtctcgcgc
    24241 gtctcctcct gcagcttgta atgatcgaac caggcgtcgg gctccagcca ggtttcggcg
    24301 accgacgtgt tgaagcaggc atggtcttcc gcgccggcgt agctttcgat cagcgtggcg
    24361 cgcgcgccgg cgtcgagcac gagcaggttg cggatgtgcg catggaaggc cggctcgccc
    24421 gacgagaaga agatgagatg aatcggggcg tcgatctgcc gcccgcgcgg gacatggagg
    24481 tagacgccgt cctcatgcag cgccagattg agcgcgatga aggcgtgctc ggcgtggggc
    24541 gcgagccggc cgagccgact cctgacctcc tccggccgct cgcgcagcgc ttcgcgcaag
    24601 ctggtcagca cgaccccggc gggcaggccc gcgatgtcgg agagcgccgg cagcaggcgg
    24661 ccgttgacga agaccagccg atgggccggc agatcgccca gcagatacgg ctggattttg
    24721 tcggcgatgg cccgctcgcc gtcggcggcg ggggcggggg cgaaggcctg atccagcagc
    24781 gggtcgagcg acgtgaagcg ccagccttcc atgcggcggg tggggaagcc gaggcgggcg
    24841 aaggcgtcgg cgccggcgcg gcgcagctcg cccagccagg ccggttcctg tccccggccc
    24901 tgggcggcaa gcgccgtcgc ctggaggaag tagtcctggc ctgtcagtcc gggagggttt
    24961 gtcgccatcg gtgtcatgtc ctgcatcgat ccgtcacgca attcaaagtc gggctaaacg
    25021 ccggcgggct gctcgaccca ggcgtagcct ttgtcctcca gttcgagggc gagttccttg
    25081 ccgcccgagc gaacgacgcg gccgtcggcc agcacgtgca cgaagtcggg gacgatatgg
    25141 ttgagcaggc gctggtagtg ggtcacgacg atcacgccgc gatcgggacc gcgctgcgag
    25201 ttgacgccgt cggcgacgat gcgcagcgcg tcgatgtcga ggccggagtc ggtctcgtcg
    25261 agaatcgcga ggcggggggc gagcaccgcc atctggaaga tttcgttgcg ctttttctcg
    25321 ccgcccgaaa agccctcgtt cagcgagcga ttgagcagcc gttcgtccat ttcgagcagc
    25381 gcggcctttt cgcgcaccag cttcaggaag tcggccgcgt cgatctcctc ttcgccgcga
    25441 tagcgccgga tggcgttgta ggccgtgcgc aggaagtaga gattgccgac gccggggatt
    25501 tcgaccgggt attggaaggc gaggaaaatg ccggcgcagg cgcgctcggc ggcgtccatt
    25561 tcgagcaggt tccgcccgtc gtagaacacc tcgccctggg tgacgttgaa gacttcgcgc
    25621 cccgccagga cgttggccag cgtgctcttg cccgagccgt tcggtcccat gatcgcgtgg
    25681 acttcgccgc cgttgaccgt caggttgatc cccttgagga tctgcttgcc atccacgctg
    25741 gcgtgcaggt ttctgatttc aagtaacgcc atatgccgat gtttactccg attcagttcg
    25801 caacgagcgt tgtttctcgg gctcgggcgc gagcgacgcc ggcttcggcg ccgggatcag
    25861 gagcgacgcc gccaccgaac ccgccagcag gatgccgatg atcccgagcg agacgccgat
    25921 cgggaaatgg ccccaatcgg tcataacttc ctgttccacg tagttgtaga gcatcttgcc
    25981 gccgatgaag atcaggatca gcgacaggcc gtagctgagg aagcggaaga gctgcaccat
    26041 gccatccagg gcgaagtaga gcgagcgcag gccgaggatg gcgaacgcgt tcgacgtgaa
    26101 gaccaggaac gggtcgtgcg tgatggcgaa gatcgccggg atcgaatcga cggcgaagat
    26161 gatgtcgctg acctcgacga cgagcaggct gacgaagagc ggcgtcgcga acagccgtgc
    26221 gtcgatgcgc gtgaagaaat gcttgccgcg gtaatcctgc gtcaccggca cgaggcgctt
    26281 gaacaggcgg atgaccgggt tcttctccgg ctcgatctcg tgatccttga ccagcatcat
    26341 cttgacgccc gaatagatca gaaacaggcc gaagacgatc gtcatccaga aaaagcggtg
    26401 caggatgacc gcccccagca tgatgaagat gaagcggaag acgagcgcgc ccaggatccc
    26461 gtagtaaagg acgcggtgct ggtagacccg cggcgtgcgg aagtaactga agatcatcag
    26521 gatgacgaac aggttgtcga cgctgagggc cttctccacg acgtagccgg ccaggaacat
    26581 cagcgccgcc tcgcgggggg tgtactgcgc gccccatgag ccgaacggat gcaggtcgtc
    26641 ccagtagaag tagagcagga cgttgaaaat cagggcgagg cagatccaga aggcgctcca
    26701 tcccagcgcc tcgcgcacgc ggataacgcg atcgtgccgg tgaaacacgc ccagatcgag
    26761 cgcgagcatc aacaggatga agaggatgaa gcctagccat agcgcgattc caagccattg
    26821 aacgggcatc gaacatcggt ctcgggaggg gaatgggggg cgtcgcgggg ttgggcccgg
    26881 acgcgaaatc cccatagcct agcattgtcg gaccccaaac gtcacccaac actcccctcc
    26941 aggctgacgc cgaggaattt ctgcgcctcg acggcgaatt ccatcgggag ctcgcggaac
    27001 acctccttgc agaacccgtt gacgatcatc gagacggcat cttcggtcga gatgccccgc
    27061 tggttcaggt agaagatctg gtcctcgccg atcttggagg tcgaagcctc gtgctcgacc
    27121 tggccggtcg ggttgcgcac gtcgatgtag gggaaggtgt gcgcgccgca gcggtcgccg
    27181 atcagcatcg agtcgcactg cgaatagttg cgggcgttct ccgccttctt gtggatctgg
    27241 accaggccgc ggtaggtgtt ctgcccgcgt ccggccgaga tccccttcga gacgatcgtg
    27301 ctgcgcgtgt cacggccgat atggatcatc ttggtgccgg tgtcggcctg ctggcggttg
    27361 ttcgtcaggg cgaccgaata gaactcgccg accgaatggt cgccctgcag gatgcaactg
    27421 gggtatttcc aggtgatggc cgagccggtt tccacctggg tccaggaaat cttggaatgg
    27481 gcgcccttgc acaggccgcg cttggtcacg aagttgtaga tcccgccgcg cccctcctca
    27541 tcgcccggat accagttctg gacggtggag tatttgatct gcgcgccctc cagcgcaatc
    27601 agttccacga cggccgcgtg caactgattc tcgtcgcgct gaggggcggt gcagccctcc
    27661 aggtagctca cgtaagcccc ttcgtcggcg atgatcagcg tccgctcgaa ctgcccggtg
    27721 ttctgggcat tgatccggaa ataggtcgac agctccatcg gacagcgcac gccgcggggg
    27781 acgtagacga acgagccatc gctgaacacg gctgaattga gggcggcgta gaaattgtcg
    27841 gtccgcggca cgaccgagcc cagatattgc cggaccagct cgggatggtc gcgcaccgcg
    27901 tcggaaaacg agcagaagat gatccccagc tcgcccagct tctccttgaa cgtggtggcc
    27961 accgacacgc tgtcgaagac cgcgtcgacg gcgacgcccg ccagcagctc gcgttcgtgc
    28021 agcggaatgc ccagcttctc gtaggtctcg agcagcttgg ggtccacctc gtccaggctc
    28081 ttgggcgcgt cggacttttt cttcggcgcc gagtaatagg agatcgcctg atagtcgatc
    28141 ttcgggtaat gcacattggg ccagtggggt tcctgcatgc cggtccagtg acggtaggcc
    28201 ccgagccgcc actcggtcag ccattcgggc tcgtgcttgc gggcggaaat cgcgcggacg
    28261 accgcctcgt cgagcccggg ggggagggaa tcggcctcaa tttccgtggc ccagccatgc
    28321 tggtactggc ggcgggtcaa ttcatccagg atggtcgtgc tggtggttgc ttcgctcatc
    28381 gtgcgggggc cttccacggg ctcaggttgg gattcagtcc gcctcggccg cggcggtcag
    28441 ggacagggtt ggcgcgccgg ccgcggcggt gcggtcggcg gggcgcggaa acatcgggcc
    28501 catcatctcg tccagggtga tctcggccag cgcatcgcgg atcgcgtggt tgatccggtg
    28561 ccaactgacg cggatcgggc agagccgctc gacgctgcaa ttggactcga cctcgccgac
    28621 gcactcggtc agcgcgatgg ggccctcgag ggcggtgatg atctcggcca cggagatccg
    28681 ggcggggggc cgggccaggc gatagccccc cttggtgccg cgttgcgatt ccagaatgcc
    28741 ggcgcgggcg agggacttga ggatcttgct gaccatcggc agggggagat gcgcggcgcg
    28801 ggagaggtcg cgggcgttgt gcggctcggc ggacttccag cccatgtagg tcagcaggat
    28861 gatgccgtaa tcggcggctt tggtgaggcg gagcatggtg cggggtcgcc ttatcgagga
    28921 ccaaaccggt ccggaatcca gcaccagtgt agcgcggcgg ggaggctgag gcaaggtgaa
    28981 agcgaaccag ttcggtccga tatcgcggcg ccagccggac ggcgtcgcga tcggcagagc
    29041 gcgggagtgt cgctccgcga agatccccat gcatccgccg aggagaactg atcgagtgcg
    29101 gccgcaatgc agccggcggg ccgcgcctgc tcggcgatgg caactggggc cgcgctgtca
    29161 ccgtggtcga ttccaccggc aagtcgttgt ggaaggttgg cgcgaagttt ggcgtcaacg
    29221 gcgcccactg gggcgactac gacggcgacg gaaccgacga aatggtcgtc ggcatgaatg
    29281 gcatgggcgg attgcaggcg tggtcggccg acggccagaa gctgtggagt gtccggttgg
    29341 gcaacgtctg ggaccaggcg attgttccgg ccagcgaggg acacccggcg cggattctcg
    29401 cgacggaagc gggcggtgcc gtccgagtgt atgacgccca gggaaacccg ctggccccgc
    29461 tgcggcccag aggggcatat tacgcgggga tgtcggcgtg ccggacgaca gggggcgccg
    29521 tgcagatcgc cgcccagagc gacgaaacga ccgtgttttt tcgacgaagc gggcaagatc
    29581 gcgtggacgg ccaccgcgcg ggaagactcc ggcgggtggc gggcgaacag cctggccgcg
    29641 ggcgatgcgg acggcgatgg agcgcctgac tgggcgttca tcgatggctc gggacggttg
    29701 gtggtggcat cgccggcggg cgagcaattt taccgcgctg gcccacgccg ccgatgtgga
    29761 gggcttcgcc atcgcctcac gggcgcccga accgggcgtg ctggtcgtgc tcgccgagtc
    29821 aatgctgagc gcctgcacgc tggcgcggta gcggcggcga ttggcgacgt ccgcccgggg
    29881 atcggcgaga gccgatcgtc tgtgccatcc ctgccgtaaa acccgcccgg tcatgacctc
    29941 agctcgcctt ggcctcctcg accgcggctt ccgcctcccg cttgcgaatc aggcggttca
    30001 ttcctttgac cacgatgaac accgtaaagg agatgatcag gaattcgatg atcgtattga
    30061 tgaactggcc atagcggatg gcgacctcgg gcagttcggc gccggtcgga tcggtcatcg
    30121 ccgggcggat cacatagccc aggtccttga attccacacg gccggcgagg tagcccaggg
    30181 ggggcatcac gatgtcgtta accagcgaat tgacgatctt gttgaacgcc gcgccgatga
    30241 taatgccgac ggccatgtcg atggcattgc ccttgacggc gaactccttg aactcctgaa
    30301 tgaagcccat ggcggtgccc tcctgtgtga gatgaaaaag gattccatcg ccggtcggat
    30361 tttccaacta catattgcag cgtcggcccg ccgggtcgga caggaaattg gggagaggat
    30421 aaaaaaagag ggccgctgcc gagagggagg atgacagcgg cccgatcagg gaacagagtg
    30481 tgcgaaaccg agccacgagg cggcgcctcg acaggctcgg ccgacaaccc acactctgcg
    30541 agagggggtt agccttcggg cgcgatccag tgcaagaatc gggcctttgc gagaagacag
    30601 ggaaaaccga caagaagggg ccggatttag gggaaatcag agtatgggac ggaccgccgg
    30661 gggcgccgat gcctcaaaat gcggcatgcg cacaggtgca cgtttctcaa attgagacaa
    30721 gagaagggaa aactgctccc aggtatttaa tcggccccgc cgctcttgaa cgattccacc
    30781 accagctcga acagcttatg ctgcgaccgg ctcgcgggca gctcgtgcag cgagcgcggc
    30841 acaagccagc ggtgggcgac gtggtgctgg cgccggaccg gcccgcgctc cgccgccagc
    30901 tcgacccgat acagctccgt cgtgatcaca tggtgcgtga aggtggaacg ggtcgacccg
    30961 aggaactccg gcgcggcctg gccgatgcgg agccgagcca gttcaaagag caccgtatcg
    31021 ggcgtcaggt tctcggggat cccaatctcg cggctgtcga gccggggcaa ctcccacatg
    31081 ccggcgaagg cgcccgtttc gccgcgctgg agcgccagca cgcggccctt gcgcaccagc
    31141 acgaccgcca cttcgcgcac ctgccggatg cgcatgccgg gcgtcggccg cggataggcc
    31201 tcgggcagac cgctggcgcg cgccgcgcac gactcaaaca acggacagac cagacagagc
    31261 ggctcgcgcg ggacgcacac cgtggcgccc agttccatca gtccctcgtt gaagtcgccg
    31321 gggcgaaccg ggtcgaccag cgcgcggctc aacgcccaca gttttttccg gcccacggca
    31381 cgcgtgaggt cttcctccag ggcaaacagc cggccgatca cgcggcggac gttgccgtcc
    31441 acgagcggca gcggcagccc cagcgcgatg gacccgaccg ccccgacggt atactcgccg
    31501 aagccgggca actcggccag ttcctcgcgg gtgcgcggca acttgccgcc atggcggcgg
    31561 acgaccgact cggccacccg cttcagcata cgcgcccggc ggtagtagcc cagcccggac
    31621 caggcgcgca ggacgtcctc ctcggaggcc tcggccaggg cctcgaacga gggaaactgc
    31681 tccagaaagg cattgaaata ggggacgacc gtcgcgacct gcgtctgttg cagcatgaat
    31741 tccgagacga cggtgccgta ctccgaacgg cgcgagcgcc agggcagatc gcggtgttcc
    31801 tcgtcgaacc agcgcaggat ccgttgccgg atggcggcaa cggcttcggg gggcacggcg
    31861 gtcggcggcg cgtccatcga tcggggtgga gggctggatt ttgttcccgg catcgcgata
    31921 gagtcgaaat cgactgcggg cggcccgtcc ccaggcagga ggacgggcgc cgcacatcat
    31981 ggcgcaacgc cgccgaaacc gcaaccgggg tgggattggg aatgatcgag cgcgataccg
    32041 gcgacgtgaa ccgaaccatc cccgctgcgg cggccgacga cccgccggcc aggctggagg
    32101 accaccgcgt cctgccgcgt ctgctggcgg aactgctggc cgggagggaa ttgcacggcg
    32161 ggctcgaccg cgacgatctg cgcgagatgt cgaaacgggc cgagcgtccc aacatctggg
    32221 ggttgccgct gcatccgatt cagcggccgt ggcccggcga gttggatctg aacggccgga
    32281 tggggcccga cggcccgctg gcgatcggcc cgcggcccga gctgccgatc ggcgtgttca
    32341 aatcgttcca gcgtcaactg cgccagacaa cctacgtttt cgtccgcggc tgggtggggg
    32401 gcgccagcgc cgatccattg gccgtcgcgg ggatttttcc ccaggaagcg gcgcatctgg
    32461 ccgccggcct gcaggagctg ctctggccga tggagcccgc aagcgaggcg gcgtgggcgt
    32521 cccgacccgc gcattatacc ttcctggcgt ggcccgacgc gattcgcggc cgcgacggcc
    32581 agccccgggt ggagatgctc atcgggcact ggccgggcga gggggttggc ctgatcgtcg
    32641 gctgcgacga tccccgcgcg ctggaggagg tggcgcgcga tgccgcacgg cgacgctggt
    32701 cggcgcgtcg cgatgtcgag ttgatccgcg gagcccttga ctgcggcggg gaagtcgcgc
    32761 ggctggtgcg ggatgccacc gaaacgggag gagccttgat cgaggagccg ctggtcgtgg
    32821 agcggggccg ggtgtcgccg ctgtggcgga atccgctcct gccggccgac cgtctgtcgc
    32881 gggccgattg gcgcgccgcc gcgctcgacc cgtgggccct gccattcgga gtttgcgaca
    32941 atcaggaggg acacctgccg ctcctgcagc cggagtatga cgaggcgacg cggcgcgcgg
    33001 ccgcgggggg gtgggttgtc gttccgcgtc gcgcggcgcc gccgcccgga ccctcccggc
    33061 cgatggccta tcggctccgc gaggcgtgaa ggccggcttc gcggcgccgc cggcgccgcc
    33121 ctgatcgggg aaaaaacagg gttcccactt ccatggcgtg agattcgccc ttttcagagg
    33181 acgaggattt tccgcgatcc ccaggttccg cggtgctcca cctggggcta tgaaaattgc
    33241 gcctcttcga ggcggcatcc ggcatccctg ccggagcggg gaatagggcg ccgggtgacc
    33301 ggtggtgtcg ctgcgctcaa ccaccggcta ctttctggac atccctgccg ggaggcggga
    33361 ggaatgaccg gcgagggcgc cggtccacac cggtggcgat cccacaccgg tggtggcgga
    33421 gcacttggct tccctcatga tcaatcggcg ggacacaacg ggaccatatg gaaggttctc
    33481 gagggtcgag gacgagggac gggcgacgaa cgttggagga tcagctgccc tgttcgtggg
    33541 cgacggaggc ggaggcgcgg gcggatttcg gattattcag gatttcacgc cagcgcaggc
    33601 aacgcacgct gtggccgccg gccgactcct caagctcggg ggtctgggcg acgcagaccg
    33661 gcgccgcgaa cggacagcgc ggatgaaacc ggcagccggg cgggaaatgc gccgggtcgg
    33721 gcacattgcc cggaatcggt tcgatggact ggcccgcgcg gacccattcc aggcgcggca
    33781 ccgaacgcat caggccctgc gtgtaggggt ggagcggcga agcgaacagc tcctcggtgg
    33841 cggcgaattc gacgatctgg ccggcgtaca tcacggcgac ctcgtcgctc gtttcggcga
    33901 tgacgcccag gtcgtgcgtg atcatcagga tcgaagtgcc ggtcttccgc tggaggtcct
    33961 ccatcaggtt gaggatctgc gcctggacgg tcacatcgag ggcggtcgtc ggttcgtcgg
    34021 cgatgagcag atcgggcgcg cagccgagcg ccatggcgat catgacgcgc tggcgcatgc
    34081 cgccgctgag ctggtgcggg tattcgcgcg cgcggcgttc ggcgtcgggg atgcggacct
    34141 gctggagcat gcgcacggcc tcgtcgaagg cgtcgcggcg cgagaggccg cgatggacgc
    34201 ggaagacctc ggcgacctgc gagccgacgg tgaagaccgg attgagcgcg gtcatcggct
    34261 cctggaagat catcgaaatt tcgttgccgc ggatgcggcg catctgtttc tcggtgcggg
    34321 tcagcaggtt gcggcccttg aagcggattt cgcccccgac gatgcggccg gggggcgacg
    34381 gcaccagccg caggatcgag agcgaggtga ccgatttgcc gcaaccgctc tcgccgacca
    34441 tccccagggt gcgcccctgg cggatgcgga aactgacgcc gtcgacggcg cggcgggtgg
    34501 cgtcgcgcgt gatgaagtgg gtccgcaggt cgttgacctc cagcagcagt tcgccttcgg
    34561 ccaggcggtc gcgggtggcc gcggcggcgc ccagcgtggc ctcgggatcg gggaacatct
    34621 cggcgggtgt gggatcgttc atgccctgtc tcgcgcggcg tccggcgggg agatgccatc
    34681 catcatggtc gagccccggt ccgcgcgcaa gggccgagga tcgccgatct ttccgcttgc
    34741 ctccacgccg gggcgtaacg gaaaatgtgg cctttgtgcc cagccgggcg acccacagac
    34801 atgctgcgcc gaggaaccac ccatggttgc gattgacgcc aacgacgccc gtccgggcac
    34861 caaactgctg atcgacggcg aactgtataa cgtcgtggaa cgggcgcacc acaagcccgg
    34921 caaggggggc gccttcgtca agttcaagct caaggggctc cggaccggca aggtgattga
    34981 tcagacggtc cgcgccggca cgaccatgga gcaggccgac atcatcaacc agacgatgca
    35041 atacgtttac caggagaatg atcagttcat tttcatggac ctgcagacct atgagcagat
    35101 gccggtgccg gccagcgtgg tgggcgacgc cgcgcagtac ctgatcgaga acaccgacgt
    35161 gcaggtgacg ctgtatgagg ggcaggtgat cgggctgcaa ttgccgccga agatggactt
    35221 caccgtgatc gaaacgatcg acgacgcggt gcgcggcaat accgcgacga acgtgaccaa
    35281 ggacgcgaag atcgaaacgg gcctgatcgt gcaggtgccg atgttcgtca agaccggcga
    35341 gaagatccgc atttcgaccg aggacggcaa gtacattgag cgcgcctagc cgggcaggcg
    35401 ggatggacac gggatcacga aagccacgaa tgagcacgaa atgagggggg acgaagggaa
    35461 aggatcgcgg gtttcgagag ccgaggacga gggacgagag atggtttcag cgtgagggac
    35521 gcgaggcgag tttttgagta agaccgttca ggcaggcgcc caggaagacg gcggcgaaca
    35581 gcatcaggaa caggcgcagc gtgatggtca tgcgccccga cccgagccag tgcatcaggg
    35641 cggcgaaggc cgcgaaccag ccgaatgaaa tcgcgagcag gacgtggcgc ggattgcgcc
    35701 actcccgccg ccggccgatc aggccggcca gccccagcag tgtcaggcca tgatacagga
    35761 gcgcggcggt ccgctcgcca agccacggat cgacctcggc cagcacccgc ggcacgagcg
    35821 cgaagcgctg ccagagcttg atgacgtagt tgcgcaggaa gtgcagcggc tcggcgagga
    35881 cataggcgcg cacttcgcgg gcgtagagct tcgagagctg atagtacgtg cggcggccgt
    35941 cgaattcggc gctgttgaac cggtccagaa tcggttgcgc gtgccactcc gccgccgggt
    36001 agacgccgta atcgggccag acgagcggat cgtagcccgg gtgattgacc gcatagagca
    36061 ggacgccgcc gttggagctg acgaggaccg gatcgccggt caccagcacg ttgcgcgcga
    36121 gccacggcag caggacgaga aacggcgccg cggcgaaaat cgtcgcctgc agccagcgcc
    36181 gttcgtcgcc gcgcagccgc cagagggcga acgcggccac cgccagcccc cagcccaagc
    36241 cgttggggcg ttgcaggttg gcgatgccga gggcggcggc cgccgccagt gcccagccgg
    36301 tcgcgcgccg gtcgcaccac gccgccagca gcgccagcac gagcagcagc agaacgaggt
    36361 agttcgcctc ggtcatcggc gtggcgaccc agtagatgaa ctcgggatag agcatccaga
    36421 tcgccacgcc gcacagcgcg gcggcgacac cgtgccagcg cgccagcagc cagtaaacca
    36481 ggcctccggc cagcaggctc aacccgagct gggtccagac cagcgcggcg ggatcgaagg
    36541 ccccgcgcac ggcatagacc gcggccgtga agagcgcctg cagcggcggc ggcacgaggt
    36601 aaaccggcgg cgggtcgcgt tcgttgtagg ccggcgtcag gccgcgcccc tccagcagcg
    36661 cctggcccgc cgagacgtag taataccagg cgtcgaaatc gagcttctgg tcgggcgggg
    36721 cgagcgggcc gcgccgctcg atttcgcggg cgcgcagcca gccgccggcg gccgtcagca
    36781 cgacaacgac cagcaaagga cataaaattc ttctcaaggt ccgccgcgcg ggcggcggcg
    36841 gcgggttcga accgggggtg gcggcgggcg cgcccgcggc ttcggcctcc ggctggccgg
    36901 caatgggttg agacgactgg tctatgccac gcgctccact tcgccggcca ccagcacatc
    36961 ggcccgggtg acggcgtttt tcttgtgcgt ccagaagcga acggtaactt tcttgtaatt
    37021 gatgatgatg tcggggtggt ggttgaagcg ctcggcggtt tcagcgacgc gattgacgaa
    37081 ttgaatcccc tccatgaacg aagggaactc gtactgcttc ttgatcgcct ggccgtcgct
    37141 ctcccagccc ggcagactct tgagcgcttc ggcgagcgcg gccgcgtcga gcacgatgaa
    37201 ttccagttcg tgggccatgc cggcgtctcc ttgtgtggtg gtcgatctgt cccattctgg
    37261 catggccggc gtcgccccgc aaccggggtg aggacgcgcg cgtgtctccc ctgcgcccgg
    37321 cccgcacaca atcgtcgatt ccgtgccggg gaaagtttag ggtgttcagg ggcaaagcca
    37381 tggccggtcc gatccgtcat caccctgcga tgagccgcgc gttgccggcg tgggcgccgt
    37441 ggctgggcct cgtcgcgttg agtctcctgt tctactggcc gctggcgctc ggccgcgtgc
    37501 cgatgggggc cgacaatacg ctgttctatg ccccgtacta cgttttccgc tgggacgacg
    37561 gcctgccgct gtggaacccg tgcctgctcg cgggcacgcc gctgttcaac gacctgcagg
    37621 ccgcgttgct ctatccgttg cgctggccgt tttatttcct ggcggactgg cgcgcgtact
    37681 acggcctgtt taattatctg cattacctgg tcgcgttcgc ggggggcatg ggtttcctgc
    37741 gcgcggcggg catcgcgccg tggccggcgc tggccggggc gatgctgttc gccgcgggcg
    37801 gccacatggc cggccggatc atcaacccga cgatcttcca cgccagttgt tggctgccgt
    37861 ggatcctctg ggccggcgcg ggcgtggggg cgcgccaccg ctggctgacg acgctggcgt
    37921 gggcgatggt gctgacgatc gcgtcgccgc acctgatgtt ctacggcgtg gtggcgatgg
    37981 ccgccgtctg gattccgctg gcggcggggc cgctgcgccg ggcgcggggc tggcgggccg
    38041 ttgccggcga cgcggccgcg cgggtcggtc atttcggcct ggccttcgcg ctggccgcgc
    38101 cgacctggat tcccggcgtg ctgcgcgcgc gcgattcgat ccgcagcacg accagcgcga
    38161 tggataatct ggccaactcg ctgggctggg gcgagatccc gcgcgcgctg ctcggcggaa
    38221 cgggcggggc cgtctacccg gagttcatcg acaaatcgct ttacatcgga cccgtcgcgc
    38281 tggcgctgat cgtgggctgg gcggcgcgtc gcgcggcgtg gcgcgatccg cgcacctgga
    38341 gcggcgccgc gctcgtcctg ctcggcatcg ggttcgcgct gggccgacac atcggcatcc
    38401 agttcgtcat gccctgggtc cccgggttca acctgctggc cgggccggcg cgcgcgctgg
    38461 tgctggcggc ggcggggctc ggcctgttgc tggcgctggc gctggccgat ctgtggcgcg
    38521 agggcgaccc ggccgcgcac ggtcgccggc ggcgcgtcgg cgtgctggcg ctgacggtgg
    38581 gcctggtctg catcgtcgtc tttacgtggc gcgcctgggg ccgggccgca tccgaggggt
    38641 ccgcgccgtt cgccgaggac tggctggcgg cgtggctgcg cgcgcccaag gccgtcccgc
    38701 ccgagctcca gccctggctg gaggccgccg tggcgctgcc ggcggcgggg atcgtgctgt
    38761 tgctgccggc gatgcgccgg cgcgccgcgg cgtgcctgat ggccgtgctg ctggcggggt
    38821 tgctgcagca tttcgtcccg cgcgtcgcgc cgccctatga aaccgccgaa gaccggcgcg
    38881 tgcccgaatc gatcgcctgg ctgacccgcc agcatgaggc cgccgcgcgg gcgggaaggc
    38941 cgttccgcgt cgcgggctac gatccgctgc ggctgcacga taccgagatg gacagccggt
    39001 tccgcgccgc aacgctgatg cccaatctgg ccaccctgta tgggctggag gatattcacg
    39061 gcttcaatcc gctgatcacg ctggactatc tggagctgtt cctgcgcacc gcggggcgct
    39121 cgccgatcaa cgatccctat cgcaacctga caatcgaccg ccccgacccg cgcctgttcc
    39181 agatcctcaa cctgcgctac ctggcggggc atccttacga gcgccgcctg accagcacgc
    39241 cccagacgct gcggccccag gcggcgacgc aaccggtggt ggattgggag gaccggggga
    39301 cgagcgagcc gatcgtcgcg tggctgttcg tgtcgctgct cgacggcgcg ggcgccgacc
    39361 cggcgaccct gccgatcggc gcggaagtcg cgcgactgct ggtggaagcc gaggagggcc
    39421 gcttcgcatt cccggtgcgg aatggcgttg aaacggcgtg gctgcatgcg cccagccgac
    39481 ccgaactgct gcggcgcgac gacttcagcg cgaccgtcaa cgatctgtgg tcgacccaca
    39541 tcgccgcgcc cgcgctggac gaccggctgg caacggccaa ttaccggggc cggatcgagt
    39601 ttggccggcc gttgcacgtg cgccggctgg cgtgggagct cgcgcagccg gggctggtgt
    39661 ttcatctggc gggccaagcc tatcgcatcg caccgccgcc ggaggaagaa gaaccgtggc
    39721 ggctggcgta cggcgcgcac tcggactggg cgccgatttt cgaatttcgc gcggcgacgc
    39781 cgcgcttcgc gttgtgggag cctgcggccg cgcagcccgc gccatcaacc gcgccgccgc
    39841 ggccgctgcc gggggtcgcc gagggcgacg gcgtcgcgct gctggaacgg ggcaatcaca
    39901 cactgcgcct gcgcgtcgag gcgccgcggc cggcgctgct cgtgctgcgc gagatctgga
    39961 cccccggctg gtcggccgag gtgaacggga ccgccgccga ggtcatccgg gttcacgaag
    40021 tgttgcgcgg cgtaccggtt cccgccggcc ggagcgacgt gacgctcgtc taccgcccgg
    40081 cgtcgtttta cgtgacgctg ctgtgcgcgc tgccgttcca tttagttggg ttgggcgtgg
    40141 ccgcgcgcga cctgcggcgg tggcgggcgc ggtccggtgg tcggggaaat gagggggcgg
    40201 gctggatggg acggaaggga tgggagaagt gagcaacgca tgctgagctt cgccccctcg
    40261 gggacggggg tcttccgcgt gagcgtcccc aggttccact gcgcttcacc tcgggcgctt
    40321 caaatgcggc cccgccgggg cggctcgcga ggatggcttt tggtgaaatt gaatcagccg
    40381 tgagagaaga cccaattccc acacgggctg ctcgtgaacc tgcggttgac aacgaggggg
    40441 caaaatggcg gtttttcgag agccgagggc gagggacgag cgccgagaac cgattccggg
    40501 gcaggccttt gggggcaatt gcgaggacgc ggacgagcga ccagagatgt attcttgagc
    40561 gagagacgat tcttcagggt ccctcaggtc ccttcgccat ggggcaggat caccatcgag
    40621 aaccagaaat tatcgatcat tttgacgatc cccatgaact catcaaggtt tgacggtttc
    40681 gcgatgtagc aattggcgta gaggtcgtag gcatagacca catcgtcctc ggactgcgag
    40741 gtggtgagca cgatcacggg gatcgaccgc agctcgggat tgagcttggc ctcgcgcagg
    40801 acttcgcggc cgtggcgtcc cggcagattc aggtccagca gaaccaggtg gggccgcggc
    40861 gcctgagcgt ggcggccgcg gcgccgcaga aagtcgatcg cctcgtcgcc gtcggtcgcc
    40921 acgtgcagtt ggcactgaag cctggccatc gcgagggctt cgcgcaccag ccggatatcg
    40981 cccggattat cctccaccag caggatgtgc atgttggcct cgggtgtggt gaccatggag
    41041 tttactcgct atcgcccgga tcgaatggtt tgacggtgat cgccggcagc gtgaagtgga
    41101 agcaggtgcc ctcgccggga accgactcca cccagatgcg gccgccatga cgctcgacga
    41161 tcttgcggca gatggcgagc ccgatgcccg tccctggaat ttcgcggttg gaatggaggc
    41221 gtttgaagat catgaaggcc tgttcggcgt aacgcgcatc gaagccgatg ccgttatcgc
    41281 gcaggctgaa gcgccagtgg tcgtcctcgc ggcgcgcgcg gagttcgacc cgcggcggct
    41341 gcttgccgcg gaacttgatc gcgttggaca gcaggttctg cagcaactgc acgagctggg
    41401 tcgagtcgcc gttgacgcgc gggaggtcgt cggcgtcgat gcgcgcgccg gtttcggcga
    41461 tcgccacctg catgttgtgc tgcgcgcggc gcaggacctc gttcatctcc accgggctga
    41521 aggcccgcgc ccgcgtggtg acccgcgaat aggcgagcag gtcgttgatc agcccctgca
    41581 tccggctggc gccatcgacg gcgaagtgga tgaactcatc ggcctcgtcg ctgagctggt
    41641 tgcgatagcg cgcttcgagc agttgcaggt agctcgtcac catgcgcaag ggttcctgca
    41701 ggtcgtgcga cgccacatag gcgaactcgc ccagttcctg gttgctgcgt tccagttcga
    41761 tcgcgtagcg ctgaagctgc gcctgcgttt gattcagttc ggcgatcttc tgctgcagtt
    41821 gctcattggc ctgcgtcaac tggcgcgtgc cggggatccg gatggtgcgc ggaaccaccg
    41881 gcagcagcag cacggccgtg gtgaacgaga tcgccgcggt ggcggccttg acgaggccgt
    41941 cgagccagta ccacggcttc cacagcgtga cgatgctgag cacatgcgtc gtgccgcagg
    42001 cgaaaatgaa cgcggcgaac atcagaaaca tccagtgata gaccagatcg gtccgccggc
    42061 gcgccaacca cagcagcatc aacgggatgg aataatacga gagcgcgatg aaggaatcgg
    42121 cgacgacgtg cagccagacc aacggccgct cccacagcag gcagaagcca tgggccatgt
    42181 attcggtatg atgcaacatg tgggggtcca tctcgccctc ctttacccgg gcggcgccga
    42241 agagggggcg atcagccggg cgcgcaacac cgttgccgcc gcggacgaac cacctcatcc
    42301 cagcgaaagg cagaccgatc cttcaggaca caaactgact tgcccggaaa tcaagacaat
    42361 caggggacgg attcgaagcc tgatgaaatt cgggaaccac tcggtttaga ttaaatgata
    42421 tccccgtcaa cgcgctagga atactttttc gattcgcccg ggcagcccgg gtgagggacg
    42481 ttgctcaagg cttggctttt gtaccattta catcgagggc gagagccgaa ttatcgaagg
    42541 gggccttcgg aggcgcttac gagaacgagg gccgaagcgg gtgggacgag gggcgcgatc
    42601 ttcgtcgtgc gcaggtttgc gtctcgtcgt tgactgagac ggcgcatccg ataagatgga
    42661 ccttcgccgc gccccgcgcc gcagccgccc gattctctag agaaaccccg cgcaatgaac
    42721 gctccctccg cccccctcgt cggcatcgtg atgggctccc gctcggactg gccgacgatg
    42781 gagcatgccg ccgcgatcct gacgcagctt ggcgttccgc acgaagtgtc gatcgtctcg
    42841 gcgcaccgca cccccgatcg cctgttccag tatgccgaga gcgcggcggg ccgcggattg
    42901 gaggcgatca tcgccggcgc ggggggcgcg gcccatctgc cggggatgct ggccgccaag
    42961 acggtactgc cggtcttcgg cgtgccgatc cagtcgcgcg cgctcaacgg cctggattcg
    43021 ctgctctcga tcgtgcagat gccccggggg atcccggtgg ccaccttcgc catcggcgag
    43081 gccggggcgg ccaacgccgc gctgcatgcc gccgccacgc tggccacgca tcacccctcg
    43141 atccgcgagg cgctcgaggc ctaccgccgc cggcagacgc aggaggtgct cgaccacctc
    43201 gacccgcggg aatcctcatg agcgaatccg cccgcgtgat cggcgtgctg gggggcggtc
    43261 atctcggact gatgctgggc gaagcggcgc gccggctcgg cctcactccg cgttttctgg
//
LOCUS       DAOPCO010000008        38031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1234_length_38031_cov_6.017616, whole genome shotgun sequence.
ACCESSION   DAOPCO010000008 DAOPCO010000000
VERSION     DAOPCO010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 38031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38031
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_38031_cov_6.017616"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLS90_01425"
     CDS             complement(<1..619)
                     /locus_tag="PLS90_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPK01093.1"
                     /translation="MKQKQSRPGETGFSRRGFLKGVGGAVGAAAVVSRVLEAPGLARA
                     DEITREGIRRVPAEGLEITLHVNGQERPVTVRPDQTLLEVLREQLGLTGTKEICDRGA
                     CGGCTVLLDGRSINSCLMLAVDAAGHEVRTVEGLVQDGRLDPVQQAFVDSDACQCGYC
                     IPGFVVRSRALLDEFPNATPQQIRDGLAGNICRCAAYARIFEAVQT"
     gene            complement(819..2192)
                     /locus_tag="PLS90_01430"
     CDS             complement(819..2192)
                     /locus_tag="PLS90_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HPK01094.1"
                     /translation="MPERAAAVHPLLRLTGWWLPFVGAVTPLVVITTLVRPYVGQSAF
                     FEVATMLALFVSALALAVGVWRPPLRPTVPIVLLLLLGVFIGLSLTASQSPLFSLKHL
                     LLPFSGVVYFALIIGHPLRRAILNRLGLGLIAVGGLLAIYGILQYNGIEFLPFSEQVR
                     KNRVIATIGHPNYLASVLGPIVFVTLGYVIERRTPAWILGGLLLVFVILTCILMAQTR
                     GVWLGLVIGFLVLVLVTLRYCLKYRVGIHIIRGFAVSGMLLIGGLTIMLFIFLPRLNE
                     EFDLRERITAVYQIKSRLYYWRAAIDMARAKPWFGQGPAMFDPLFWIYALEQQKSELG
                     AYYRDVIPAISETTPGHTHNEYLQVYAENGTLGLSALVAFLAFFIYFGYFAIMRQRQA
                     DRALQGAAMYGALLMILADAMFAFPWRLPVSLIVFIYVLAWHYDEIHSGADADQVALT
                     ESAGAPA"
     gene            complement(2235..3101)
                     /locus_tag="PLS90_01435"
     CDS             complement(2235..3101)
                     /locus_tag="PLS90_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01095.1"
                     /translation="MPEAPTPPASAVAPEPAPLAGGAARPRTPLPYLVLAIVLMLLAV
                     AAGGWTLLLVSVEYRFLRVAAPTDNVDDRTFLRSYSNMLLGLLKLDPSNGRIRNQYAS
                     VISRLGDRREAVEELNRARLTENLQGTLFFLADMYEKLGDLTRAEQLMADCVIINPTN
                     PSFTPTYLRMLSNQLHPLRALKEQKDLTEEQRRHYRERYEELLGRHGLAARDWAVRAP
                     NDPNSHLFMGNFYVDPLFGVQAYRCYLMGLSRPRWMVLNRNNLFMTPAETMTTINQII
                     RGQYAFPYLDLP"
     gene            complement(3120..5819)
                     /locus_tag="PLS90_01440"
     CDS             complement(3120..5819)
                     /locus_tag="PLS90_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK01096.1"
                     /translation="MGRNNLFKLGRARRAAAARALGWTLLLGCVLLASCQKKRGPVGE
                     VPRQAVRIDCIERGVYRVTGQDLLDSGIDLNAVDAARMTLVKERASVPLLAVGLEDDW
                     FDPQDEIYFFAEGAVRDERPYINIRGEYDPVVESFMLHLYPSEHEPQRYRKASTRAPA
                     KSLPRDYPVRVVEGRIHFERDDVWEFFEAGAWDEQRSDFLFWKKLTYPASDKTESDFT
                     HEFAVPGAVTREPARLRIMLLGLSEVEGIDRRITHHVRVQFNEMAPEEIVWNSRFPHL
                     AELDLPPRSLREGINRLKVSVLPPTEGETDAKQSDVLKIDVVMLDWFQIDYRQHTLAH
                     EDRVEFDVVTPPAGSDAIKCAVDNFSTDQAFAFDLTERTFRDLRGYPSDLRRGQYSVN
                     LDLAPTSRTLVMLTPDKALTPFRLEATTIKGLFDQPSDAQLLIVSHPDFLEALEPFVE
                     WKRSRGLKTELVNAADLFNESLGGVVGPEAIRDYIRHVYESQAEPALKYVLLVGDSTT
                     IAKYQTHLPAYSFLQSGEHTNDNYFANFSDPKGDPQIAVGRFSVRTVEQLNHAIRKTL
                     DYESHKYDGLWRSRFFLIAASDSWAHRDAQRLIDEFARPHYLVNSIRTNKANTLPGEH
                     EKLTQQLAQALNEGSLITAFFGHGGGTVWEVGAATRAEYFRAHLFDQENVATLKNLHK
                     PSLVFALTCYTNDFDNPHVRQTLGETFVNSPGGAVAVIGATGRSSTGLNYMYIRQFMD
                     LLSSGTQTRLGDLFVECKRHYSVPAMNATYVLLGDPSLEFSLNRNLVEVTVEPVGAEG
                     EFALNYGLPPDTPLPVKLQAFLIDENENLVETWTSEVTELSGTLNYNFSGGAWSDTMR
                     MVVYATDGKALHITGGVMLAGIRRNLRIAAGDAPTTATTAAHP"
     gene            6141..8333
                     /locus_tag="PLS90_01445"
     CDS             6141..8333
                     /locus_tag="PLS90_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPK01097.1"
                     /translation="MPEQRPESLGRPRPPQPPATQVKGDAPEDPSQASGYLTQPSSNS
                     SPEEPTLVERDKLASSASAGPSSTGGPTSPPTDPLGSLPQGLRDRYKMLRVLGKGGFA
                     NVYLAQDQVLDQKVAIKILKLGLASDSDRERFLFEARVGAKLRHPNIAAVFDIIQTPD
                     GLQMIMEYYPGGTLSDRLKKKGALNWRVSLDIARQVAQALDYAHKRQFVHRDVKPANI
                     FLSDDGLVRLGDFGIAKQTEHHEYTQTGMIIGTPLYMAPEQATDSRNVDPRTDIYALG
                     LTLYHMLTGVPPRVLDLDLVPRPFRRLIKAATAPDRNERLVSASQFIALLDQIGQEME
                     QETRARVGQTPPGSLPRVGPAGASDTGATPSSASTQSDILSTPTNPPSVPPTTENRTP
                     TLAPVYLEDGTAAETPLKTEVKPEVEPPPVAELDPFAELGGVQPSPPAAPLMHHAQAP
                     PRSAGARHLWIIAAVALIGAGAGAIGVWGLLGGGATSEVADARIVADEPALPPVATAP
                     PAPTGPSAAIDPAPTPSPTEPTPSPAAAAPGVMPGASTPAATNAPTPSAAVAAVQPTP
                     EPTPVGPPTLADAMQQLREENKAIERIFEQFGYLRKDRTSGVLHLPQIQLSLDEQLRE
                     SPDDPRLHLIQCYVWMWRSDMRSAHQSMIEAARLNHERTEPYPFHDAALREALEMEAN
                     EPPLPPPGPPRAPGAAQPRLGMPGPQGGGPPVMGRPGARTPAGPRRPQ"
     gene            complement(8368..8880)
                     /locus_tag="PLS90_01450"
     CDS             complement(8368..8880)
                     /locus_tag="PLS90_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01098.1"
                     /translation="MSNLTDASQSRIILMPAAEAIGPWRTLALALARGGHRLAFADAS
                     QGLDDQLRDLQFEVERAGGISLAGPADDPAWAREAFGALDLVLTPSPGEAAREAERCF
                     KGQPKATGGRNPAPLRRVVLLTPEPGAGEPCVVERTSAGTGAPLWRVSAPAPLPTRAA
                     EQLAERITQP"
     gene            9192..10955
                     /gene="recJ"
                     /locus_tag="PLS90_01455"
     CDS             9192..10955
                     /gene="recJ"
                     /locus_tag="PLS90_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPK01099.1"
                     /translation="MALPLRQMPAPAAAPKKPAPPRWQIRPVDDAVCRNLTQALELPP
                     LVARLLACRSITQPDAAEHYLRPVLAQLHDPFLMKGMEPAAERIVHAIRNGEPICIYG
                     DYDVDGISATAILMTILRELGAEVTYYIPDRFSEGYGVNNEALEQIAGEGARVVITVD
                     CGIRAVDQAELARRRGIDLIITDHHEPGPRIPPAFAVINPRQPGCAYPDKGLAGSGVA
                     FKLAHAVLRKAHPDGAAARELLKSTLDFVALGTVADVVPLLGENRAMVSFGLKLLRET
                     RRPGLLHLFQHAAIDPRKLQTHTIGFGIGPRLNAAGRTDHAMFGLELLLSRDARAADG
                     LAAKLEQFNANRRAIEMDILREAVELIEPRAHERVLVVAREEWHHGVLGIVASRIVQR
                     YYRPTIVIGITDGVGKGSGRSIEGFDLHAALESCGGHLLQFGGHMMAAGLSVAADAVE
                     AFRAAINAHAEQVLPDDLLRPLLWIDAAARTEDLTEEFVNQLELFEPFGQDNPRPVIA
                     LDEVALIEPPRVLKDRHLKLMVAGRDGRPLCAMGWGMADRLHELNADGTVLRLAGAPC
                     LNRWNGRVSVEMELRDFQVVG"
     gene            complement(10965..20105)
                     /locus_tag="PLS90_01460"
     CDS             complement(10965..20105)
                     /locus_tag="PLS90_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK01100.1"
                     /translation="MLEIDSILQSINEQIDGEQLARLIDYHPDKIRSDGKTLKCFCPV
                     HREMAFRSLIFYIKQHNYKCMMKQCELFEGGTFVEFWSIFKRLEPIEAALDLAERLKL
                     DVDVGRVRLLGEEFAIAAREALEQARYDEAHAKIQQALAFDVNNLQLRLLSAQIHEAR
                     GDVEAALAERYGLYEYHLGQHDLDLARTVLDDIRAHYPPSPELFRRRVELAERQGDTD
                     GIQAVLIELSEYQEKQGDIQAAAAALEDALARDTENTDVLDLLARFHHRHGNRKAERQ
                     TLERLAVVFEGQTEARRLLGVLDRLLALNPDDLSVRERIAAAMARSGQTDSARKQWLA
                     IARSREEQALFAEAEQIVARLLVTDPESVDLLEVQARLLNESGRRAEAVRTYRQLAAL
                     ARDVEMDTRVNEYYELARAIDPGDLGLRKDLAEWKLVRGDLEGGLNELFNLADFSLAN
                     GHRAEGMAILTRIATLAPTDLDKRLRIGRCLERNGLADEAFASYSLLVRDLMKQQQFD
                     AAQAICEEVRRLRPGDTETLVLRIETHLALDQKNEAIEACREFARERMVEDDFVAAEA
                     ALERGIRIDRTEKAAKTDLAQLYEQTGRARRAAALWIEIALFERAQGQTDAANGAVRE
                     ALRLDPQSNEARVMLAEGLEAAGEIREAAELWKQILVEQREAGAAPEELHARLLHLLS
                     LAPRDHQVLGELAPLSLRLEGAEAALPYYIRWLEALEAQGLAEEMMSAYGRAVEAYPN
                     QQTWRRRLAAMLMEAGREEEASEHLEKLLEGMEAEGRGDEEQHLTLEQLVALKPERHD
                     LRAQLARSLARQGQAHEAARMFEEIADLRLRDGDLDQGIALMKEALEAAPGNHDLLER
                     TAGLIERAGRMEDALELYERLAELNRRKSERARSIPILEKLLAYQPERLELRAEIAEL
                     YEAEGDIDRALEEYLTLARAFNRQDSSPQRVVELCQRIRGLMPECIEARELLVESWLK
                     QGHTAGARQELEQLGDWALSVNDLQRAEGFYRRIQEIEPGDIGNGERLARLYEASGRD
                     REAQAAYQHVLELYEQAGEGASAIRVLHKLRDLDEENLELRLRLARALLNEPGQRREA
                     AEEWLALIERCGEQAPERLTALLDEALPSFRTDWDWRQRVIRLLSLQPDYEGIEAAWE
                     TLARDALEAREGVIAREAAAEGLRRNPDSLVLRRLRVDANRALSNYDLAIEDLRELAE
                     HAGARNDYEGAAALLREGLELQPRNGALLRMLAEAQLAAGHTAEGRASLTSLIALHEQ
                     EGDFEQAIQRARQLVRISGQSPESRDALAVLLMKAGQTRESVEEWCRLAEELAESGQE
                     EAAMARYLRILEYLPGDVEILRRLADLCYDTGGMVMAMGHYDRLLDAMQASCEPAAVE
                     AEILRILELESGHLALKERLAEFLQQQGRTEESRRVLREIFATYRDERGEHRSALRVL
                     RQLKKLDPSDTQAQREEAELLEATGNAREAAQVWQLLALEHRQRRSLAEAAACADRAA
                     ALLADRPELQLEAAGLYEDLDDIERATECYLRAVAVHEAQGHHGACIAILERLVEINP
                     RRHDLPERLARLLEAEGQTDQAGRVWLSLGQREESAGRLLEARRIYQHLRQLLPDSVE
                     PRRRLARICEREGDRPGAIVQWRELAELCGRRGENEWEAEFLQHLLELDPRDPAALER
                     LATLWRDASRPHALYDVLDRLESLHRAAGRFDLALRTLEEMKGLRPKEPDLQARAVEL
                     LLKTGREEEGARQALELIAVWFEAGEDSRALEMLRRVAEINPNDIERRIMLARMIHDH
                     GRIEAARQEFFLTSTKLFGEMQWEACRHACEAGLELFENDVRLRDLLGRALIKLGRRG
                     EAIESQLHLATLYEERGEAVRAQRVYESILEMQPDHLATLEAMVEWALRHDRPALAVD
                     NLLRLSEAHYLAGDLERATRSMEQIARIDPNRAELMARLAELYLEGDRPEDARRTWIE
                     SARHMLREGRYDAAVEALERCHKHDPDSLEVHIELVHAYAQADRLEDYRREGLRLAGL
                     CRDRGEIDRALEVYRGLTEYDPHDLMAWEHLAELLEAQGLTTEASQAHYTQYELHRAA
                     RRPDRARLCLERALALQPDSLVLLETLGELCLAQGRRAEGLTHLAQAATQLHHDGHHP
                     RAIKLAERILKLEPDNLEIVRLRAHALQAEGDLTGALEGFIQAARGLSETHENDAAIE
                     VLEELLALAPELHDQRELYARVLRRQGRVRESVEQYLKLIASLGPDDDPRKAIRFCRQ
                     ILTDSPDEPAAHACLCDVYARCDKPRQAYQECLWLARHHREQKNLAEAERYVELGLGW
                     FPDDLGLRRELVDILLAAGRTTDASVQLGELARVAEARADAQLTIWAMEKASELEPDN
                     VEHRRRLADQLERGGDAEGARRTRIELIRRMLDLNLMDDARRLAEQVASGLSDEDPRR
                     REIGEIFEAAGLPEVAAYHFHLLARGYATAGKLDQARSLAGRVLELKPRHVGAREILI
                     EVLLGQGERAAAAEQYEKLAEIYSEAGQWEGALRTLEALIELIPDRTRPRLRLAELYE
                     RLQREDALAEQLWRLVELYSGAGRKTEAVECLRRIIEHQPENTQARLRYIEYCREEAS
                     PTEMAEAYQELANLYARKGAVDKAAEMYEQLTALDSRSTEGRDQFIQFLLAQGDIDRA
                     LEQTVALLDLLEEGGRTAEAVRVVERAINYAPQATDLRHRLARYHLSLNRRGVALETF
                     RSLARQYEQAGDVAHLVEVLESIVDIDPLSVEHRQRLAELYHRRGQDEAAVEHYERLA
                     EQYLDGGLSDLAEHTLRRVLALRPGRSDLWERVIAIHQQEGRTDEALADLIMLADLYM
                     EEAQLEQALTTYKRILELDPDNIEVLNLYIDLSLQMGPETVVVDEMLRLADLRARRGE
                     AREAMSIYRHLMELCPHHEEIKERMSATQTLVRDGGALKTDPAGSTLGILGASDPNTV
                     TKLIRNYENILRLNTRNPAARAKLAELMERAGQHEEADRHWLAAAEDYVQKGNLERAL
                     EICGHQIERHPDDPRMREMQSRIMVQRDSMRIIDGALSDELDKL"
     gene            complement(20511..21941)
                     /gene="glnA"
                     /locus_tag="PLS90_01465"
     CDS             complement(20511..21941)
                     /gene="glnA"
                     /locus_tag="PLS90_01465"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPK01101.1"
                     /translation="MEARDCKTPKDVIQVVKEKGIKVVDYKFMDFPGLWQHFSQPAWM
                     LEEETFSDGVGFDGSSIRGWQAIHESDMLLIPDPMTALIDPFCQAPTLSLVCNVVHPG
                     TMEKYTRDPRNIALKAEAYLKSTGIGDTCYIGPEAEFFIFDDVRFDSQPNQSFYLVDS
                     IEGRWNTGRDEGPNLGYKPRYKEGYFPCPPADHFQDLRTEMMLTMIDCGIPIEAQHHE
                     VATGGQAEIDMRFAPLTHQADCLLLFKYIIKNTARKYGKTVTFMPKPLYGDNGSGMHT
                     HISVWKDGKNLFAGKSYAGLSELALHFIGGLIKHAPSILAFAAPSTNSYKRLVPGYEA
                     PVNLAYSQRNRSAACRIPMYSPSEKAKRVEFRPPDPSCNPYLTFSAMLMAGLDGIQNK
                     LDPGDPLDKNLYDLPPEEAKLIKKVPGSLDEALAALEADHDYLLKGDVFTEDVIDEWI
                     SYKTKMEIDAVRMRPHPYEFHLYYDI"
     gene            22877..24559
                     /locus_tag="PLS90_01470"
     CDS             22877..24559
                     /locus_tag="PLS90_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01102.1"
                     /translation="MLLDDLTRLACPACGGALRVDDAHARFADDLPPGVDPAAMAWEG
                     ALACLACHSSYPLLEGVACLARLDSRWLIPLKEMDSRLSLSLRTIEQHPWEAGRAQAY
                     AEQERIVWDEMAALFAAALDELDLQPGVTVLDVGAGEAWTTQEFQRRGARVVALDCDL
                     AQLRYLSFHDIEGSAPSWRDAPYIHPGSGARFFWKDPKPRDRYFTRVFGDIGTLPFPS
                     ASFDIVFCRSVLHHLADHERTLREMARVVKPGGRIVVCAEPIRSILTDEEDCYDQCVQ
                     REEGMNERVWPAARFLDPVRPYADHLLVKVWPNPPARSLRRRLPLIAPLLARRIGPGA
                     RFRGPKLALLNWIDASINLLGRRNDAPLPTVWAGPPSPEDQAALHELVEVFRVRPGEI
                     EAGVEDLPRKREVVGRLRRELLARGPQLPRAIDLARAAVPQLDRGWLDVVTHDGQPGR
                     RMAREAAVTLSARTEAGQPCTTLRVRAARHGPVTARLVIHANGRRALPIELNGPSWVE
                     ARVPLPFTREPVVTLRLRAEHASAGYYGAPGAEGAPPFGAEPVIAVGELALE"
     gene            complement(24587..26113)
                     /locus_tag="PLS90_01475"
     CDS             complement(24587..26113)
                     /locus_tag="PLS90_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01103.1"
                     /translation="MARTKNPQPAARREGEPSRERFEELCERFDHGIALVDSRGILRW
                     CNSVLVDFYLQADSPAAWQGKRAQDLFRLLFQGSDGWSRIRAALRRLHHSPKGRVEIA
                     DLAVTWGGRPQTYVQLVICALKPGAGRSIGWEAWYFYDVTSHRKSELNLQALLQHSTD
                     GIFAVDSECRLRVFNEACERLTGWRAEEVLAGQGLESPLFRCAHPACSRFSDAGESGG
                     FFCARAQESVPRGERPLVSRTGETFWVEVTYAPMYDSQGRIALVLGIMRDVTQRHQLE
                     EQLRVTRQLATLGELASAVAHEIKNPLGIIHSSAEILINPNRPDEQKRQAAEFIRDET
                     RRLDDRIQAFLKFSRPKPPDMKRQSIHKVLTQTIIAYQTLAREGLTIETHYEHNLPHV
                     HIDADQMQQVFLNLIMNADQAMDSRGVIRIRTGMHGDHQIAIEVRDHGPGIREEDAEH
                     LFELFYSTKPRGTGLGLPIVMQIVLAHGGQIEARNCPDGGASFVVTLPAAADGDEAGA
                     "
     gene            26390..26620
                     /locus_tag="PLS90_01480"
     CDS             26390..26620
                     /locus_tag="PLS90_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01104.1"
                     /translation="MLALIRGEVFQAPLATSFSENDWAICRIRLPAGLPTAAAYAIRG
                     HNAAQPDQAGWTPRFTIHARNAAQPRAWPEME"
     gene            complement(26640..29807)
                     /gene="glnE"
                     /locus_tag="PLS90_01485"
     CDS             complement(26640..29807)
                     /gene="glnE"
                     /locus_tag="PLS90_01485"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HPK01105.1"
                     /translation="MSAAPFRSEPADPQALAEPLTEALTAGREEEGRRLLGALGFAEP
                     GDAWRHLDALCAELPEAPRRELLRGLTPALAAAADANQALLDLLRYGEARFGREQRAL
                     LATVPAPTLAILCRLFGFSQFLADLLIRYPQFFEWLEEDPELGQPRTLERCLRQYELA
                     LGPFRRPAARRDAAIRCQRRELLRLGVRRMIGLSDEMQMAGELSELAAATLRVALREA
                     YGPLEERHGTPMDDAEPGAAPTAAGFCVIGMGKLGGGELNFSSDIDVIFVYSAEGTTT
                     GGADGAPAISNHQFFTRLAEAMLSWLTEHGPEGYLYRVDTRLRPEGESGPLTRSLAAC
                     EIYYATQARTWERLALLKARPVAGDERVARGFEALRRPFVFNPLHAEGLVGQVYELKG
                     LIDGEVARRGDAGREIKRGRGGIREIEFLVQTQQLLHGGARPGLDSPSTLEALAALAR
                     EGLMPREEAERLRSDYIFLRTLEHRLQMEQLRQTHRLPDNPRELEALARRCGLTPRPG
                     ATPREQLQAAWRETTERVHAAFAKFFLPQGAEARPAPAPADEAERATRAVLSAEPEGN
                     VLPALQPFELADGSALKTLRRLAGMGRTVYLSVSEQQQFERLLPILLRCLQRAPQPRV
                     ALAHFESFTEACGARAVFYETFAGAQPLLELLLKAFGMGEALAQTLIAHPEFVDYLSD
                     LSLIAGPADPGAMGQRLNRWLQGARDEEAFCQALAYFRRFEYLMASLGELAGLLDYTA
                     YCERLVATAERVLEQALARAAAERGLPPLPAGFAVMALGKLGGRELGFASDLDLIFVW
                     EEPFGAGAGSAAEAAAALAERVLFFLTHVSAEGRAYAIDARLRPEGSGAPLAPHLTSY
                     EEYYAQRAQPWELQTAMKLRWVAGDRALGARLTETVRERVLRRLPELRIGEEIRSMRR
                     RIEDSVRNLPRWVFADFKKGRGGLMDLEFIAQHLQLTHLRDHPALLGLGTRRVLDYAR
                     EQGLLEAGQVDAIARDYIWCRRVELRARLLSGSDKTIIPSGGEKLAALERACRGELLA
                     EGTTLQDEMARRLVRNRRHFEQIVR"
     gene            30069..33191
                     /gene="secA"
                     /locus_tag="PLS90_01490"
     CDS             30069..33191
                     /gene="secA"
                     /locus_tag="PLS90_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPK01106.1"
                     /translation="MLNFIFRWLVGDKRQRNFKQVAPIIAQINDWCERYNSLTDDELK
                     AKTTEFRERLAQGATLDDLLPEAYGAVKQVCRRLVGQSWEAAGIPINWNDVPFDVQLC
                     GAIYLHRGNVCEMATGEGKTLVATMPLYLNALEGKGAHLVTTNDFLARFGKEWVSPVY
                     ERLGMTTGVILSQMTPAERREAYNCDITYGTNNEFGFDYLRDNMALQPEHLVQREHHF
                     AIVDEVDSVLIDEARTPLIISGQVDRSTHQFDRLKPLVVRLVEKQNQLVNSLVAEAER
                     LLEGTDPDKVDDRRYEAGMKLLQARLGSPKHKRFTKIASDGAIRRLIDRVENDFMRDK
                     RMRELEEALFFVVDERGHTIDLTELGRQTISPDNPDLFLLPDLVEEFAQLENQGLSEE
                     ELERKKEELMNRHEERTEELHNISQLLRAYVLYERDVDYVVQDNKVVIVDEFTGRLMT
                     GRRWSDGLHQAVEAKEGVTIEKETQTLATITLQNYFRMYKKLAGMTGTAETEAEEFAH
                     TYKMDVYVIPTNVPIRRLDLNDLIYRTEREKFNALVQEVHRLHEYGLPVLVGTTSVNT
                     SEKLSIMLRRQKISHSVLNAKQHEREAQIIRQAGQLGAVTIATNMAGRGTDIKLGQGV
                     IKCADGAKPPGGKYCVACPNRPPGAEVDPDMPPCGLQILGSERHEARRIDRQLRGRAG
                     RQGDPGSSRFFVSLEDELMRLFASERMAKLMSKGFVEGEAMSHRIATRALESAQKKIE
                     SINFEQRKRTLDYDNVMNKQRERIYGIRREALLGQGSNLERVLEFTGEALADAWEKHC
                     PGNDESEWDVTGFLDWVRRYVPLIDLNNLPPLSVDSVDDYLSEILERCAAVYRRKTEM
                     MGEQMTGALARYVLLTIIDTHWRDHLLAIDELRHGIALRSYGQKDPLTEYQREATLLF
                     EEMMAAVMREIFERIYRVYVVQEPTTGTARLTYLKEEGRQSITDAARSAAAQAAQAAE
                     GQAQQGQTSEGERPARPRTYRREQPKTKPNDPCWCGSGKKYKKCHGNPALQQAPLASQ
                     PAGPTDDDAA"
     gene            33175..34149
                     /locus_tag="PLS90_01495"
     CDS             33175..34149
                     /locus_tag="PLS90_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK01107.1"
                     /translation="MMTPPDPGLTLLHFGDLHLWRIGLDADPRFKRLLGLANLLLRRG
                     RLFPLSVARLVLRRIEHDPADYLCFSGDLTTTSLKAEFEDARRLLLPLLDKWQARFLA
                     IPGNHDRYTVRAAGRRYYETLFAGEHARFPFEVDLDAVWTLIGIDCSVPRRFTSRGRF
                     TREARDALAPRLAEHRRRGRRLIVMGHYPLLYPARVPVQLDHALPERMALFELLAEHR
                     VDAYLHGHKHQRWRLKHEGVTLLNCGSAGMDHPRHRPGYLRIQLTHDGIGAVQSVSLR
                     HRADIVREARREARAHEAAHDHAPPDTGELPAGEWVTRLLEEDGGAVH"
     gene            complement(34180..36078)
                     /locus_tag="PLS90_01500"
     CDS             complement(34180..36078)
                     /locus_tag="PLS90_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01108.1"
                     /translation="MMHCPRFRRFAALAAAALLPALFQPVQADKIVTTQGQSLQGVIV
                     DENVYNIYLDTGGVVVPIPHERVKIVERLDVEDNARQLLERAQEAIQNGDLSTARTLL
                     EQVRRLTVTDEHLLAQRLRLDTELTELERHGGTLAQRRENARDLLRRAQEAYDRIDDD
                     LGNDLLIQSLLIDPGLPEAHELIGRRLTARGKPDLLLAGEYFGQILWPDNLRPDSPIV
                     PLLPQVYRYLIELFLTTKDDVRAPHYAQLVSKLNEAFLLHPEWKNAPGIDAATRELLE
                     RPFDQVLAEQVQINLNAGEYGLAVGKLQGWADPSVSPEMALLIARANTGAGHYDAAIA
                     ALEVATARFPRDVSLTPQLNALKLLAEGARQEQQGNLAEATEFYEKVFANRLQLVEEI
                     GQAVGSRLATIKAPQMDTATGSRGDWRLADVAALVMNFGSDLGQQRRGARTLSQQLPL
                     APWHLKPTLQVNGADLPTTAAIPLAIAQGLSRPLAVRFNEESPFELTFRINLVCSPED
                     YQRLGTSVDREGRFEINPPVQINNITFDLVAFHPTLGILLEEDWSPPDVAEPVKQWRA
                     QQPNFPAADHPVYLYLDSATKLAQFLSNEMPYYLIPEVTILPSRLNLPRMRELLTTPR
                     ATPVGAAP"
     gene            36614..37612
                     /locus_tag="PLS90_01505"
     CDS             36614..37612
                     /locus_tag="PLS90_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPK01109.1"
                     /translation="METTSQSPESARKSCCAPARGEENPALPAAERPVAPAGGPPPSG
                     MVRLPGGSFLMGTDYPEGFAEDGEGPVREITLSPFWIDQTAVTNAQFARFVEPTGYKT
                     DAERYGWSFVFHLLLPPGTLKRVTLRVQGCEWWCRVRGACWRHPEGIGTSIAHRLDHP
                     VVHVSWRDAAAYAAWAGKRLPTEAEFEYAARGGLVQKLYPWGDDLTPGGEHRCNIWQG
                     EFPRINTAEDGYVGTAPVTAYPPNGFGLYQMTGNVWEWCWDEWSRDDHARPEAPRVNP
                     AGPPPSPDHPRKVMRGGSYLCHWTYCNRYRIAARTANTPDSSTGNIGFRCVMDLST"
     gene            complement(37727..>38031)
                     /locus_tag="PLS90_01510"
     CDS             complement(37727..>38031)
                     /locus_tag="PLS90_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyl-tRNA--protein transferase"
                     /protein_id="HPK01110.1"
                     /translation="LDLTARCASSIYACFDPELAPLSPGTLSALREIESARERGIPYY
                     YLGYAIHGNPSMRYKLEFRPNEVFDGAEWRPFRDADGRYRFDPAAFRAGRWAAELG"
BASE COUNT         6044 a        14007 c        11847 g         6133 t
ORIGIN      
        1 ccgtctggac cgcttcgaaa atccgcgcat acgccgcgca gcggcagatg ttgcccgcca
       61 ggccgtcgcg gatctgttgc ggcgttgcat tggggaattc atccagcagc gcgcgcgacc
      121 ggacgacgaa cccggggatg caatagccgc actggcaggc gtcgctgtcg acgaacgcct
      181 gctgcaccgg atccagccgg ccatcctgaa ccagcccctc caccgtgcgg acctcgtgcc
      241 cggctgcgtc gaccgcaagc atcaggcagc tattgatcga gcgcccgtcg agcaggacgg
      301 tgcagccgcc gcaggcgccg cggtcgcaga tctccttcgt cccggtcagg ccgagctgct
      361 cgcgcaggac ctcgagcagc gtctgatcgg gacgcaccgt gaccggccgt tcctggccgt
      421 tgacatgcag cgtgatttcc agaccttcgg cggggacgcg ccgaatgcct tcgcgcgtga
      481 tttcatccgc gcgcgccagg cccggcgcct ccagcacgcg gctgacgacg gccgccgcgc
      541 cgaccgcgcc gccgacgccc ttaagaaatc cgcgacggga gaagccggtc tctcccggtc
      601 gactctgctt ctgcttcatg gggaccgcct cccgcactcg gggatgttgc gtcggctcag
      661 cggccgatgg gcaacgcgcc gccggtcggg cggtgggaat cagatccgga ggcgccgacc
      721 ggccggggag atcgttggtg gagacatcat agtatgccgg cggcgctccg acaagcccgt
      781 tgaccgcacg tcgaatcgcc cgcccgcccg gagttcgttc acgccggcgc gccggccgac
      841 tcggtcaatg caacctggtc ggcatcggcg cccgaatgga tttcgtcgta atgccaggcc
      901 agcacgtaga tgaagacgat cagactgacc ggcagccgcc agggaaaggc aaacattgcg
      961 tcggcgagga tcatcagcag cgccccatac atcgccgcgc cctgcagcgc gcggtcggcc
     1021 tgacgttgcc gcatgatcgc gaagtagccg aagtagatga aaaaggccag gaacgccacc
     1081 agcgcgctca gccccagcgt cccattctcg gcgtagacct gcagatactc gttgtgcgtg
     1141 tggccgggcg tcgtctcgga aatcgccggg atgacgtcgc gatagtacgc ccccagctcc
     1201 gacttctgct gctccagcgc gtagatccag aagagcggat caaacatcgc ggggccctgg
     1261 ccgaaccacg gcttcgcgcg cgccatgtca attgcggcgc gccagtaata cagccggctc
     1321 ttgatctgat agaccgcggt gatgcgctcg cgcaggtcga attcctcgtt cagccgcggc
     1381 aggaaaatga acagcatgat cgtcagtccg ccgatgagca gcatgccgct gaccgcgaat
     1441 ccccgaatga tgtggatccc gacgcggtac ttgaggcagt aacgcagtgt caccagcacc
     1501 aggaccagga acccgatcac cagtcccagc cagacaccgc gcgtctgcgc catcaggatg
     1561 caggtgagga tcacaaacac gagcaacagc ccgccgagga tccacgccgg cgtccgccgc
     1621 tcgatcacgt agcccagcgt gacaaagacg atcggcccca gcaccgaggc aaggtagttc
     1681 ggatggccaa tcgtcgcgat cacgcggttc ttccgcacct gctcgctgaa cggcaggaac
     1741 tcgatgccgt tgtattgcag aatcccgtag atcgccagca gcccgccgac cgcgatcagg
     1801 ccgagaccca gccggttcag gatcgcgcgg cgcagcggat gcccgatgat gagcgcaaaa
     1861 tagacgacgc cgctgaacgg cagcagcagg tgcttcagcg agaagagggg cgactgcgac
     1921 gccgtcaacg acaggccgat aaacaccccc agcaacaata gcaggacgat cggaaccgtc
     1981 ggccgcagcg gcggccgcca gacccccacc gccagcgcca gcgcgctgac aaacagcgcc
     2041 agcatcgtcg ccacctcgaa aaacgccgat tggccgacgt aggggcgcac cagcgtcgtg
     2101 atcacgacca gcggcgtgac cgcgccgacg aacggcagcc accagccggt caggcgcagc
     2161 agaggatgca cggcggcggc ccgctcaggc atggctggcc gctccttcat gacgcatgcc
     2221 ggcgaccggc gggctcatgg cagatccaga tacgggaagg catactgacc tcggatgatc
     2281 tggttgatcg tggtcatggt ctccgccggc gtcatgaaca gattgttccg gttcagcacc
     2341 atccaccgcg ggcgggacaa ccccatcagg tagcagcggt aggcctgcac gccgaacaac
     2401 ggatcgacgt agaagttgcc catgaacaga tgggaattcg ggtcgttcgg ggccctgacg
     2461 gcccagtcgc gggcggccag gccgtggcgc cccagcaact cctcataccg ctcgcgatag
     2521 tgccgccgct gctcctcggt caggtccttc tgctccttca gcgcgcgcag cgggtgaagc
     2581 tggttgctca gcatccgcag ataagtcggc gtgaagctgg ggttggtggg gttgatgatg
     2641 acgcagtcgg ccatcagttg ctcggcccgc gtcaggtcgc ccagtttctc gtacatatcg
     2701 gccagaaaga acagcgtgcc ctgcaggttt tcggtcagcc gcgcgcgatt gagttcctcg
     2761 accgcctcgc gccggtcgcc cagccggctg atcacgctgg cgtactggtt gcggatccgg
     2821 ccattgctcg ggtcaagctt caacaggccg agcagcatgt tgctgtagga gcgcagaaac
     2881 gtccggtcgt cgacgttgtc cgtcggggcc gcaacccgca ggaaacgata ctcgacgctg
     2941 acgagcagca gggtccagcc gcccgcggcg acggccagca gcatcagcac gatcgccagc
     3001 acgaggtacg gcagcggcgt gcgcgggcgc gcggcgcccc ccgcgagcgg cgccggctcg
     3061 ggtgcgactg cggatgctgg cggagtgggc gcttcaggca tgctgcttgg accggcgaat
     3121 cagggatggg cggccgtcgt cgcggtcgtt ggcgcgtcgc cggcggcgat ccgcaggttc
     3181 cggcgaatcc ccgcgagcat gaccccgccg gtgatatgca gcgccttgcc gtcggtcgcg
     3241 tagacgacca tccgcatcgt atcggaccac gccccgcctg aaaagttgta attgagcgtc
     3301 ccggacaatt ccgtcacctc gctcgtccag gtctctacca ggttctcgtt ctcgtcgatc
     3361 aggaaggcct ggagcttgac gggcagcggc gtatcgggcg gcaggccgta gttcagcgcg
     3421 aattcgccct ccgccccgac cggctccacc gtcacctcga ccaggttgcg gttcagcgag
     3481 aactccagcg agggatcgcc cagcagcacg taggtcgcat tcatcgccgg cacgctgtag
     3541 tggcgcttgc attcgacgaa caggtcgccc agccgcgtct gcgtgccgct gctgagcaga
     3601 tccatgaatt ggcggatata catgtaattg aggccggtcg agctgcggcc ggtggcgccg
     3661 atgaccgcaa ccgccccgcc cggcgagttg acgaacgtct cccccagcgt ctgccggacg
     3721 tgcgggttgt cgaaatcatt cgtgtagcac gtcagcgcga acaccagcga cggtttgtgc
     3781 agattcttca gcgtggccac gttttcctgg tcgaacaagt gcgcccggaa gtattcggcg
     3841 cgggtcgccg cgcccacctc ccagacggtg cctcccccgt gcccgaagaa ggcggtgatc
     3901 aggctccctt cgttgagcgc ctgcgccaac tgctgcgtca gcttctcgtg ttcgcccggc
     3961 agcgtattgg ccttgttcgt ccggatgctg ttgaccagat aatgcgggcg ggcgaattcg
     4021 tcgatcagcc gctgggcgtc gcggtgcgcc caactgtcgg aggccgcgat caggaagaac
     4081 cgcgagcgcc acagcccgtc atacttgtgg ctctcgtaat ccagcgtctt gcggatggcg
     4141 tgattcagct gctcgacggt ccggaccgag aaccgcccga cggcgatctg cggatcgccc
     4201 ttcggatcgc tgaaattggc gaagtagttg tcgttggtgt gttcgccgct ctgtagaaac
     4261 gaataggccg gcagatgggt ctgatacttg gcgatcgtcg tggagtcgcc caccagcagg
     4321 acgtacttga gcgccggctc ggcctgcgat tcgtagacgt gccggatgta gtcgcggatc
     4381 gcctcgggcc cgaccacgcc ccccaggctc tcgttgaaca gatcggccgc gttgacgagt
     4441 tcggtcttca gtccgcgcga gcgcttccat tccacgaacg gctccagcgc ctccaggaaa
     4501 tcgggatggc tgacgatcag cagttgcgcg tcggaaggct ggtcgaacag ccccttgatc
     4561 gtcgtggcct ccagccggaa cggcgtcagc gccttgtccg gcgtcagcat gaccagcgtt
     4621 ctggaggtcg gcgcgagatc cagattgacg gaatactgtc cccgccgcag atccgacgga
     4681 tagccgcgca gatcgcggaa cgtccgttcg gtcagatcga aggcgaaggc ctggtcggtg
     4741 ctgaaattat cgaccgcgca cttgatcgcg tccgacccgg ccggcggcgt gaccacatca
     4801 aattcaaccc ggtcctcgtg cgcgagcgtg tgctgccggt aatcgatctg gaaccagtcg
     4861 agcatgacga cgtcgatttt gaggacatcg ctctgcttgg cgtcggtttc cccctccgtc
     4921 gggggcagca cgctgacttt gagccggttg atcccctcgc gcagcgagcg cggcggcagg
     4981 tccagttcgg ccagatgggg aaaccggctg ttccagacga tttcctccgg cgccatctcg
     5041 ttgaattgca cccgcacgtg gtgcgtgatc cgacggtcga tcccctccac ctcggagagt
     5101 cccagcagca tgatccgcag gcgcgccggt tcgcgcgtca cggcgcccgg aacggcgaat
     5161 tcgtgcgtga aatcggactc ggtcttgtcg ctggccggat acgtcagttt cttccagaac
     5221 agaaagtcgc tgcgctgctc gtcccatgca cccgcctcga agaactccca gacgtcgtcg
     5281 cgctcgaaat ggatccgccc ctcgacgacg cgaacgggat aatcgcgcgg caggctcttc
     5341 gccggcgccc gcgtggaggc tttccgatag cgctgcggct cgtgctccga cgggtagagg
     5401 tggagcatga acgactcgac gacgggatca tactcgccgc ggatgttgat atacggccgc
     5461 tcgtcgcgca ccgccccctc ggcaaagaag taaatctcgt cctgcgggtc gaaccagtcg
     5521 tcctccagcc cgacggccag cagcggaacc gacgcgcgtt ccttgaccag cgtcatccgc
     5581 gccgcgtcga cggcgttcag gtcaatcccg ctatccagca gatcctggcc ggtgacccgg
     5641 taaacgcccc gttcgatgca gtcaatccgg accgcctggc gcggcacttc gcccaccgga
     5701 ccgcgtttct tctggcaact cgccagcagg acgcagccga gcagcaacgt ccagcccaac
     5761 gcccttgccg ccgcggcgcg gcgcgcccgg cccagcttga ataagttgtt ccgtcccatg
     5821 aatccgcgat gcagccgacc gattcgcaag agatcgcccc tcgatatgga accccgcgct
     5881 gccccttctc cccttagagt aacgagcccg acgccatggc tgggcttcaa tccggatggt
     5941 ccgattctaa cgcatcccgt cgcgcggacg caagccggaa tcgcccccgg cacggtggaa
     6001 atccggcccc gccgattcgg ccggatccac ccccgcgggc ggcacggccg cgccagacct
     6061 tcgacgttcc gcactcgaac cgcccgcccg cctgtgctac gctgaacggc taacgatgga
     6121 tcccgttcgg aggaagcggc gtgcccgaac aacgcccgga atcgctgggc cgaccccgtc
     6181 ctccccaacc gcctgccacc caggtgaaag gggatgcgcc ggaggaccca tcccaggcct
     6241 ccggctacct gacgcaacct tccagcaatt cctcccccga ggaaccgacg cttgtcgagc
     6301 gcgacaagct ggcttcgagc gcctccgccg gccccagttc aaccggcggc ccgacctccc
     6361 cgcccaccga tccgctcggc agtctgcccc agggcctgcg cgaccgctac aaaatgctgc
     6421 gcgtcctggg caagggcggt ttcgccaatg tctatctcgc tcaggaccag gtcctcgacc
     6481 agaaggtcgc gatcaagatc ctgaagctcg gtctggcctc cgacagcgac cgcgagcgtt
     6541 tcctgttcga ggcgcgcgtc ggcgccaagc tgcgtcaccc caacatcgcc gccgtcttcg
     6601 acatcatcca gacgcccgac ggcctccaga tgatcatgga gtattacccc ggcggcaccc
     6661 tgagcgatcg cctcaaaaag aagggcgcgc tcaactggcg ggtctcgctc gacatcgcgc
     6721 gtcaggtggc tcaggcgctc gattatgccc ataaacgcca gtttgtccat cgcgatgtca
     6781 agcccgccaa tattttcctg tccgacgacg gcctggtccg gctgggcgat ttcggcatcg
     6841 ccaagcagac cgaacaccat gaatacaccc agaccgggat gatcatcggc acgccgctct
     6901 acatggcgcc cgaacaggcc accgattcgc gcaacgtcga cccgcgcacc gatatctacg
     6961 ccctcggcct gacgctctat cacatgctga cgggcgtgcc gccgcgcgtc ctcgatctgg
     7021 atctcgtccc gcgcccgttc cgccggctga tcaaagccgc gacggccccc gaccgcaacg
     7081 agcggctcgt ctcggccagc cagttcatcg ccctgctcga ccagatcgga caggaaatgg
     7141 agcaggaaac gcgggcgcgc gtcggccaga cgccccccgg ttcgttgccg cgcgtcggcc
     7201 cggccggagc ttccgacacc ggcgccacgc cgtccagcgc ctccacgcag tccgatattc
     7261 tgtccacgcc caccaatccc ccctccgtcc cgccgacgac ggagaatcgg acgccgacgc
     7321 tcgcgccggt ttatctggaa gacggcacgg ccgccgagac gcctctgaaa accgaggtca
     7381 agcccgaagt ggagccgccg ccggtcgcgg agctcgaccc gttcgcggaa ctgggcggcg
     7441 tccagccttc tccgcccgcg gcccctctga tgcatcacgc gcaggcaccc cccaggtcgg
     7501 ccggcgcgcg gcatctatgg atcattgcgg cggtggcgct gatcggcgcc ggcgccggcg
     7561 cgattggcgt ctggggcctg ctgggaggcg gagcgacgtc ggaggttgcc gacgcgcgga
     7621 tcgtcgccga cgaaccggca ctgccgccgg tcgcgaccgc cccgccggcc ccgaccggcc
     7681 cgtcggccgc catcgaccct gcgccgacgc cttccccgac tgagccgacc ccttcgccgg
     7741 ccgccgcggc gcccggcgtc atgcccggcg cgtcaacgcc ggcggcaacg aacgctccca
     7801 cgccgtcggc cgccgtcgcg gcggttcagc cgacgcccga gcccacgccg gtcggcccgc
     7861 cgacgctcgc cgatgccatg caacagttgc gcgaggaaaa taaggcgatc gagcggatct
     7921 tcgagcagtt cggctatctg cgcaaggatc gcacctccgg cgtcctccat ctgccgcaaa
     7981 tccagctgag cctcgacgag caattgcgtg aatcacccga tgatccgcgc ctgcacctga
     8041 tccagtgcta tgtctggatg tggcggagcg acatgcgcag cgcccatcaa tcgatgatcg
     8101 aagcggcccg gctcaaccat gaacgcaccg agccgtatcc gttccatgat gcggcgctcc
     8161 gcgaggcgct ggagatggag gcaaatgagc cgccgcttcc cccgccggga ccgccccggg
     8221 cgcccggcgc cgcgcaaccg cgcctgggga tgcccgggcc gcaaggcggc ggcccgcccg
     8281 tcatgggccg ccccggcgcc agaaccccgg ccggccctcg ccgcccacag taagttcaac
     8341 acgtccctcg tcctcgcgat cgacccctca cggttgcgtg atgcgttcgg cgagttgctc
     8401 ggccgcccgc gtcggcagcg gcgccggcgc gctcacccgc cacagcgggg cgcccgtgcc
     8461 ggcgctcgtc cgttcgacga cacacggctc acccgctccg ggctcaggcg tcaacagcac
     8521 gacccgccgc agcggcgccg gattcctccc gccggtcgcc ttgggctgac ccttgaaaca
     8581 gcgttcggcc tcgcgcgcgg cctcgccggg cgacggcgtc agcaccagat ccagcgcgcc
     8641 gaacgcctcg cgcgcccagg ccggatcgtc ggcgggtccg gccagcgaga tcccgccggc
     8701 acgttccact tcgaattgca gatcacgcag ttggtcgtcc aggccttgcg acgcgtcggc
     8761 gaacgccagc cggtgtcccc ctctggcgag cgccagcgcg agcgtgcgcc acggcccaat
     8821 cgcctccgcc gcgggcatca gaatgattcg ggattggctc gcatcagtca ggttgctcat
     8881 ccaaaatccg tccctcgact ctcggccctc gtcctcgtcc tcgtcatcgc ccccaacgac
     8941 ccacccaaga ttcggctctc gactctcggc cctcgtcctc ggctctcgaa aaagctcgcc
     9001 attttgcccc ctcgatacga acctccggtc acgacccgcc cggccgcgtt tgacttcacc
     9061 cggcccgctc gttacactgg ccgtcgccgt aagcggggag ccgcgatcca ccttccggat
     9121 cgctcttctg cctgacgggg ttcaacccgg tcgcgcccgc tcccgtccca cctgctgctc
     9181 gaggtatgcc gatggcgctc ccgcttcgcc agatgccggc gccggccgcc gcgccgaaaa
     9241 aaccggcgcc gccgcgctgg cagatccgcc ccgtcgatga tgccgtctgc cggaacctga
     9301 ctcaagcgct ggaactcccc ccgctcgtcg cgcggttgct ggcctgccgc agcatcaccc
     9361 aacccgacgc cgccgagcat tacctgcgtc ccgtcctcgc ccagctgcac gatccctttc
     9421 tgatgaaggg catggaaccg gccgccgagc ggatcgtcca cgccatccgc aacggcgaac
     9481 cgatctgcat ctacggcgac tatgacgtcg acggaatcag cgcgacggcg atcctgatga
     9541 cgatcctgcg cgagctgggc gccgaggtca cgtactacat ccccgatcgc ttcagcgagg
     9601 gctacggcgt caacaacgaa gcgctcgaac agatcgccgg cgaaggcgcg cgcgtcgtca
     9661 tcaccgtcga ctgcggcatc cgcgccgtcg accaggccga gcttgcgcgc cgccgcggca
     9721 tcgacctgat catcaccgac caccacgagc ccgggccgcg gattccgccg gcgttcgcgg
     9781 tcatcaaccc gcgccagcct ggctgcgctt accccgacaa ggggctcgcc ggttcgggcg
     9841 tcgcgttcaa gctggcgcac gccgtcctgc gcaaggccca tcccgacggc gcggccgcgc
     9901 gcgagctgct caagagcacg ctcgatttcg tcgccctcgg cacggtagcc gacgtggtgc
     9961 cgctgctcgg cgagaatcgc gcgatggtca gcttcggctt gaaactgttg cgcgagacgc
    10021 gacgccccgg cctgctccac ctgttccagc acgccgcgat cgatccgcgc aaactgcaga
    10081 cgcacaccat cgggttcggc atcggaccgc gcctgaacgc cgccggccgg accgatcacg
    10141 caatgttcgg cctggaactg ctgctcagcc gcgacgcccg cgcggccgat ggcctggccg
    10201 ccaaactgga acagttcaac gccaaccgcc gcgcgatcga gatggatatc ctgcgcgagg
    10261 cggtcgagct gatcgaaccg cgcgcgcacg agcgcgtcct ggtggtggcg cgcgaggaat
    10321 ggcatcacgg cgtcctcggc atcgtcgcct cgcgcatcgt gcagcgctac taccggccga
    10381 cgatcgtgat cgggatcacc gacggcgtgg gcaagggctc gggccggtca atcgaaggct
    10441 tcgacctgca cgccgcgctc gaatcctgcg gcgggcacct gctccagttc ggcggtcaca
    10501 tgatggccgc cgggctctcg gtcgcggccg acgccgtcga ggccttccgc gcggcgatca
    10561 acgcgcatgc cgagcaggtg ctgcccgacg atctgctccg tccgctgctc tggatcgatg
    10621 ccgccgcccg caccgaggac ctgacggagg agtttgtcaa tcagctcgag ctgttcgagc
    10681 cgttcggcca ggataatccc aggccggtga ttgccctgga cgaggtggcg ctgatcgagc
    10741 ccccccgcgt cctcaaggat cggcatttga agctgatggt cgccggccgc gacggccgcc
    10801 cgctgtgcgc gatgggctgg ggcatggccg accggctgca cgagctcaac gccgacggga
    10861 cggtgctgcg cctcgccggc gcgccctgcc tcaaccgctg gaacggccgg gtctcggtcg
    10921 aaatggaact gcgcgatttt caggtcgtcg gttgatccgc ggcgctacag cttgtccagt
    10981 tcgtccgaaa gcgcgccgtc gataatgcgc atcgagtcgc gctgcaccat gatccgcgac
    11041 tgcatctcgc gcatccgcgg atcgtcgggg tgccgctcga tctggtgccc gcagatctcg
    11101 agcgcccgct ccaggttccc cttctgcacg taatcctcgg cggcggccag ccagtgccgg
    11161 tcggcctcct cgtgctgccc ggcgcgctcc atcagctcgg ccagcttggc gcgcgccgcc
    11221 gggttgcgcg tgttgagccg caggatgttc tcataattcc ggatcagttt ggtcacggta
    11281 ttcggatcgc tggcgcccag aatccccagc gtgctccccg ccgggtccgt cttgagcgcc
    11341 cccccgtcgc gcaccagtgt ctgcgtcgcc gacatgcgtt ccttgatctc ctcgtggtgc
    11401 gggcacagct ccatcaggtg gcgatagatc gacatcgcct cgcgcgcctc gccccgccgg
    11461 gcgcgcaggt cggccaaccg cagcatctca tccacgacga cggtctcggg acccatctgg
    11521 agcgagagat cgatgtagag gttgagcacc tcgatgttgt ccggatcgag ttccagaatg
    11581 cgcttgtagg tcgtcagcgc ctgctccagt tgcgcttcct ccatgtagag atccgccagc
    11641 atgatcaggt cggccagcgc ctcgtcggtc cgcccttcct gctgatggat cgcaatgacc
    11701 cgttcccaca gatcgctgcg gccggggcgc agcgccagca cgcggcgcag cgtgtgctcg
    11761 gccaggtccg acagcccgcc gtccagatac tgctcggcca gccgctcgta gtgctccacc
    11821 gcggcctcgt cctgcccgcg ccggtgatag agttcggcca gccgctgccg gtgctcgacg
    11881 ctcagcggat cgatgtcgac gatcgattcg agcacctcga ccaggtgcgc cacgtcgccg
    11941 gcctgctcgt actgccgcgc cagcgagcgg aaggtctcca gcgcgacgcc gcggcggttc
    12001 aggctcaggt ggtaccgcgc cagtcgatgg cgcagatcgg tggcctgcgg ggcatagttg
    12061 atcgcgcgct cgacgacgcg cacggcctcg gccgtccggc cgccctcctc cagcagatcc
    12121 agcagggcga cggtctgctc cagcgcgcgg tcgatgtcgc cctgcgccag caggaactgg
    12181 atgaactggt cgcggccttc ggtcgagcgc gaatcgagcg ccgtcagttg ctcgtacatc
    12241 tcggccgcct tgtccaccgc cccctttcgc gcatacaggt tcgccagctc ctgatacgcc
    12301 tcggccattt cggtcggcga ggcctcttcg cggcaatact cgatgtagcg cagccgggcc
    12361 tgcgtgttct cgggctggtg ttcgatgatc cggcgcaggc actcgaccgc ctcggtcttg
    12421 cggccggcgc ctgagtagag ttcaaccagc cgccagagtt gctcggccag cgcatcctcg
    12481 cgctgcaggc gctcgtagag ctccgccagg cgcaggcgcg gccgcgttcg gtctggaatc
    12541 aattcgatca gcgcctcgag cgtgcgcagc gccccttccc attgccccgc ctcggaataa
    12601 atctcggcca gtttctcgta ttgctcggcg gcggcggccc gttcgccctg acccagcagg
    12661 acctcgatca ggatttcgcg cgcccccacg tgacgcggct tgagctccag cacccggccg
    12721 gccagcgacc gcgcctgatc cagtttgccc gccgtcgcat agccgcgcgc cagcaggtgg
    12781 aaatgatagg ccgcgacctc gggcaggccg gcggcctcga aaatctcgcc gatctcgcgc
    12841 cgacggggat cttcatcgct cagaccgctg gcgacctgct cggccagccg ccgcgcgtcg
    12901 tccatcaggt tcaggtccag catgcgccgg atcagctcga tccgcgtccg ccgcgccccc
    12961 tcggcgtctc ccccgcgctc cagttgatcg gccaggcgtc ggcgatgctc gacattgtcc
    13021 ggctccagtt ccgaagcctt ctccatcgcc cagatcgtga gctgcgcatc ggcgcgcgcc
    13081 tcggccacgc gcgccagctc gccgagctgc accgaagcat cggtcgtccg gcccgccgcg
    13141 agcagaatat cgaccagctc gcgccgcagc cccagatcgt cggggaacca gcccagcccc
    13201 agctcgacat agcgctcggc ctcggccagg ttcttctgct cgcggtgatg ccgcgccagc
    13261 cacaggcact cctgatacgc ctggcgcggc ttgtcgcagc gggcgtaaac atcgcacagg
    13321 caggcgtgcg ccgccggctc gtcggggctg tcggtcagga tctgtcggca gaagcggatc
    13381 gccttgcgcg ggtcgtcgtc cgggcccagc gacgcgatca gcttcagata ctgctcgacg
    13441 ctctccctga cccggccctg gcgacgcagg acgcgcgcgt acagctcgcg ctgatcgtgc
    13501 agttcgggcg ccagcgccag cagctcctcc agcacctcga tcgcggcgtc gttctcgtgc
    13561 gtttcgctca ggccgcgcgc cgcctggatg aagccctcca gcgcgccggt cagatcgcct
    13621 tcggcctgca aggcatgcgc gcgcagccgc acgatttcca gattgtccgg ttcaagcttc
    13681 aggatccgct cggccagctt gatcgcgcgc gggtgatgcc cgtcgtgatg caactgagtc
    13741 gccgcctgcg ccaggtgcgt caggccctcg gcgcgccgcc cctgcgccag gcagagctcg
    13801 ccgagcgtct ccagcagcac caggctgtcg ggttgcagcg ccagcgcccg ctccaggcag
    13861 agtcgcgcgc ggtcgggccg ccgcgccgcc cggtgcagct catactgcgt gtaatgcgcc
    13921 tgcgacgcct cggtcgtcag cccctgcgcc tccagcagtt cggccaggtg ctcccacgcc
    13981 atcaggtcgt gcggatcata ctcggtcagc ccgcgataca cttccagggc gcggtcgatc
    14041 tcgccccggt cgcggcacag gcccgccagc cgcagccctt cgcggcgata gtcctcgagc
    14101 cggtcggcct gggcatacgc atgcaccagt tcgatgtgca cttccaggct gtccgggtcg
    14161 tgcttgtggc accgttccag cgcttcgacg gcggcgtcgt aacgcccctc gcgcaacatg
    14221 tgccgcgccg attcaatcca ggtgcgccgc gcatcctccg ggcgatcgcc ctccagatac
    14281 agttcggcca gccgggccat cagctcggcg cggttgggat cgatccgcgc gatctgctcc
    14341 atgctccgcg tcgcgcgttc caggtcgccc gccaggtagt gcgcctcgct cagccgcagc
    14401 aggttatcga ccgccagcgc cggtcggtcg tggcgcaggg cccactcgac catcgcctcc
    14461 agcgtggcca ggtgatccgg ctgcatctcc aggatgctct cataaacgcg ctgcgcgcga
    14521 accgcctcgc cgcgctcctc gtagagcgtg gccaggtgca gttgcgactc gatcgcctcg
    14581 ccgcggcggc ccagcttgat cagcgcgcgc ccgagcaggt cgcgcagccg aacgtcgttc
    14641 tcaaacagct ccagccccgc ctcgcaggca tggcggcacg cctcccactg catctcgccg
    14701 aacagcttgg tcgaggtcag gaagaattcc tggcgggcgg cctcgatccg gccgtgatcg
    14761 tggatcatcc gcgccagcat gatccgccgc tcgatgtcgt tggggttgat ctcggcgacg
    14821 cggcgcagca tctccagcgc gcggctgtcc tcgcccgcct cgaaccagac ggcgatcagc
    14881 tccagggcct ggcgcgcgcc ctcttcctcc cgcccggtct taagcagcag ttccaccgcg
    14941 cgcgcctgca ggtcgggctc cttcggccgc agccccttca tctcctccag cgtgcgcagc
    15001 gccaggtcga accgccccgc cgcccggtgc aggctctcca gccggtccag cacgtcatac
    15061 agcgcatgcg gccggctcgc gtcgcgccag agcgtggcga gccgttccag cgccgccgga
    15121 tcgcgcggat cgagctccag caggtgctgc agaaattcgg cttcccattc gttttcgccg
    15181 cgccggccgc acagttcggc cagctcgcgc cactgcacga tcgcccccgg ccggtcgcct
    15241 tcgcgctcgc agatccgcgc cagccgccgc cgcggctcca cgctgtcggg cagcagttgc
    15301 cgcagatgct ggtagatccg ccgcgcttcg agcagccggc ctgcggactc ttcgcgctgg
    15361 cccaggctga gccacacccg cccggcctgg tcggtctggc cctcagcctc gagcagccgc
    15421 gccagccgtt cgggcaggtc gtggcgccgt gggttgatct ccaccagccg ttccagaatc
    15481 gcgatgcacg cgccgtgatg cccctgcgcc tcgtgcaccg ccacggcgcg caagtaacac
    15541 tccgtcgccc gctcgatgtc gtccagatcc tcatacagcc cggccgcctc gagctgcagt
    15601 tcggggcggt cggccagcag cgccgccgcg cggtcggcgc acgccgccgc ctcggccagg
    15661 ctgcgccgct ggcggtgttc cagcgccagc aactgccaca cctgcgccgc ttcgcgcgcg
    15721 ttgccggtcg cctccagcaa ctcggcctct tcgcgctgcg cctgtgtgtc ggagggatcc
    15781 agcttcttga gctggcgcag gacgcgcaac gcgctgcggt gctcgccgcg ctcgtcgcga
    15841 tacgtggcga aaatctcgcg cagcacgcgg cgcgattcct cggtccggcc ctgctgctgc
    15901 aggaattccg cgagccgctc cttcagcgcc aggtggcccg attccagctc cagaatccgc
    15961 aggatctcgg cttccaccgc cgccggctcg cacgaggcct gcatcgcgtc gagcagccgg
    16021 tcgtagtgcc ccatggccat caccatgccg cccgtgtcgt agcacaggtc ggccagacgg
    16081 cgcaggatct cgacgtcgcc ggggaggtac tccaggatgc gcaggtagcg cgccatcgcc
    16141 gcctcttcct gccccgactc ggcgagttcc tcggccagcc ggcaccattc ctccaccgac
    16201 tcgcgcgtct gccccgcctt catcagcaga acggccagcg cgtcgcgcga ttcgggcgac
    16261 tgcccgctga tgcgcaccag ttggcgcgcg cgctgaatcg cctgctcgaa atccccttcc
    16321 tgttcgtgca gcgcgatcag gctggtcagg ctcgcccggc cctcggccgt gtggcccgcc
    16381 gccaactgcg cctcggccag catccgcagc agcgcgccgt tgcgcggctg cagttcgagg
    16441 ccctcgcgca gcagcgccgc cgccccctca tagtcgttgc gcgcgccggc gtgctcggcc
    16501 agctcgcgca ggtcctcgat cgccaggtcg tagttcgaca gcgcgcggtt ggcatccacg
    16561 cgcagccggc gcagcaccag gctgtccgga ttgcgccgca gcccctcggc cgccgcctcg
    16621 cgcgcgatca ccccctcgcg cgcctccagc gcgtcgcgcg ccagcgtttc ccacgccgcc
    16681 tcgatcccct cgtagtccgg ctgcagcgag agcagccgga tcacccgctg ccgccagtcc
    16741 cagtcggtcc ggaagctggg cagcgcttcg tccagcaggg ccgtcagccg ttccggggcc
    16801 tgctcgccgc agcgctcgat cagcgccagc cactcctcgg ccgcctcgcg ccgctgaccc
    16861 ggttcgttca gcagggcgcg cgccagccgc agccggagct ccaggttttc ctcatccagg
    16921 tcccgcagct tgtgcagcac gcggatcgcg ctggccccct cgcccgcctg ctcatacagc
    16981 tccaagacat gttgatacgc cgcctgcgcc tcgcgatcgc gccccgaagc ctcatacagc
    17041 cgcgccaggc gctcgccgtt gccgatatcg cccggttcga tctcctgaat ccggcggtaa
    17101 aaaccctccg cgcgctgcag atcgttgacc gacaacgccc agtcgcccag ttgctccagt
    17161 tcctggcgcg ccccggccgt gtgcccctgc ttcagccagc tctccaccag cagctcgcgg
    17221 gcctcgatgc actccggcat cagtccgcgg atgcgctggc aaagctccac gacgcgctgg
    17281 ggcgagctat cctggcggtt gaaggcgcgc gccagcgtca aatactcctc cagcgcgcga
    17341 tcgatgtcgc cttcggcctc atacagctcg gcgatctccg cgcgcagttc cagccgttcg
    17401 ggctgatacg ccagcagctt ttccagaatc ggaatgctgc gggcgcgctc cgatttgcgc
    17461 cggttgagct ccgccagccg ttcgtagagt tccagcgcgt cttccatccg cccggcgcgc
    17521 tcaatcagcc ccgccgtccg ctccagcaga tcgtgattgc ccggcgccgc ctccagcgcc
    17581 tccttcatca gcgcgatccc ctggtccaga tcgccgtcgc gcagccgcag gtcggcgatc
    17641 tcctcgaaca tgcgggccgc ttcgtgcgcc tgaccctggc gcgccagcga tcgcgcgagc
    17701 tgcgcccgca ggtcgtggcg ttccggcttg agcgccacca gttgctccaa cgtcaggtgc
    17761 tgctcttcgt cgccgcgccc ctcggcttcc atgccctcca gcagcttttc gagatgctcc
    17821 gaggcttcct cctcgcgccc ggcctccatc agcatcgccg ccagccgccg ccgccacgtc
    17881 tgctggttgg gataggcctc caccgcccgg ccataggccg acatcatctc ctcggccagc
    17941 ccttgggctt cgagggcttc gagccaccgg atgtaatacg gcaacgccgc ctcggccccc
    18001 tccagccgga gcgacagggg cgccagctcg cccagaacct gatggtcgcg cggcgccagg
    18061 ctcagcaggt ggagcagccg cgcgtgcagc tcctcggggg cggcccccgc ctcgcgctgc
    18121 tcgacgagaa tctgtttcca cagctccgcc gcctcgcgaa tctcgccggc cgcctcgagc
    18181 ccctcggcca gcatcacccg cgcttcgttg ctttgcgggt ccagacgcag cgcctcgcgc
    18241 accgcgccat tggccgcgtc ggtctggccc tgggcccgct cgaaaagcgc gatctcgatc
    18301 cacagcgccg ccgcgcgccg cgcgcgcccg gtctgctcgt acaactgcgc caggtcggtc
    18361 ttagcggcct tttccgtccg gtcgatccgg atcccgcgct ccagcgcggc ctcggccgcg
    18421 acaaaatcat cctcgaccat ccgttcgcgc gcgaattccc ggcacgcttc gatcgcctcg
    18481 ttcttctggt ccagcgccaa gtgcgtctcg atccgcagca cgagcgtttc cgtatcgccc
    18541 ggccgcagcc gccgcacctc ctcgcagatc gcctgcgccg cgtcgaactg ctgctgcttc
    18601 atcagatcgc gcaccagcag cgaatagctg gcaaacgcct cgtcggccag gccgttgcgc
    18661 tccaggcacc gcccgatccg cagccgcttg tccagatcgg tcggcgccag cgtcgcgatc
    18721 ctggtcagaa tcgccatccc ctcggcgcgg tggccgttgg cgagcgaaaa atccgccagg
    18781 ttgaacagct cgttgagccc gccctccagg tccccgcgca ccagcttcca ctcggccagg
    18841 tccttgcgca gtcccaggtc gcccgggtcg atcgcccgcg ccagctcgta atactcgttg
    18901 acccgcgtgt ccatctccac gtcgcgggcc agcgcggcca gttgccggta ggtgcggacc
    18961 gcctcggccc gccgtcccga ctcgttcagc agccgggctt gcacctccag caggtcgacg
    19021 ctctcggggt cggtcaccag caggcgcgcc acgatctgtt cggcttcggc gaacagggcc
    19081 tgttcctcgc gcgaacgcgc gatcgccagc cactgcttgc gcgccgagtc ggtctggccg
    19141 ctgcgcgcca tcgccgcggc gatccgttcc cgcaccgaca gatcatcggg attgagcgcc
    19201 agcagccgat ccagaacgcc cagcagccgg cgcgcctcgg tctgcccctc aaagaccacg
    19261 gccagccgct ccagcgtctg gcgctcggcc ttgcggttgc cgtggcgatg gtggaaccgc
    19321 gccagcaggt cgagcacgtc ggtgttttcg gtgtcgcgcg cgagcgcgtc ctccagcgca
    19381 gccgccgccg cctgaatgtc cccctgtttc tcctggtatt cactcagctc gatcagaacc
    19441 gcttgaatgc cgtccgtgtc gccctggcgc tcggccagct ccacgcgccg gcggaacagc
    19501 tcgggcgaag gcgggtagtg cgcgcgaatg tcgtccagca ccgtgcgcgc caggtccaga
    19561 tcatgctggc ccaggtggta ttcataaagg ccatagcgct cggccagggc ggcttcaacg
    19621 tcgccgcgcg cttcatggat ctgcgccgac agcagccgga gctgcaggtt gttcacgtcg
    19681 aacgccagcg cctgctggat cttcgcatgc gcctcgtcgt agcgcgcctg ctcgagcgct
    19741 tcgcgcgcgg caatggcgaa ttcctcgccc agcagccgca cgcgccccac gtcgacatcc
    19801 agcttcagcc gctcggccag gtccagcgcc gcctcgatcg gttcgagccg cttgaagatc
    19861 gaccagaatt caacgaacgt ccccccctca aacagctcgc actgcttcat catgcacttg
    19921 tagttgtgct gcttgatgta aaaaatcagc gaccggaacg ccatctcgcg atggaccggg
    19981 cagaaacact tcaatgtctt gccgtcgctg cggattttgt ccggatgata gtcgatcagg
    20041 cgggcgagtt gctcgccgtc gatctgctca ttgatggact ggaggatgga atctatttca
    20101 agcatcgccc acacacgtcg ccgaccccat aacccgacgg gccggaccac aggtcatctc
    20161 gccagggaca cccctctacc atcctttcgt cccggcgggt gatggggatt aggtcctttt
    20221 tcccagggcg acagttttca gggggcgcgt tcgtcccgac ttgagcccgc cacaaaattc
    20281 aggcggattt tggatcctta tgtccctatc tcgcaaaatc ctaccatccc gtaggattcc
    20341 gggctccctt cttaccgctg cgtagccttc ctcggcccga ggctccactc cctccttttg
    20401 tcccttatgt cccttctgtt cttttccccc gataaaacga agcccgccga ccccatgggc
    20461 cggcgggcaa ctcccctccg acgtgcggat tcgatccgcc gcgacgtcgg tcagatgtcg
    20521 taatacaggt ggaactcata cggatgcggc cgcatccgca cggcgtcgat ctccatcttg
    20581 gtcttatagc tgatccactc gtcgatcacg tcctcggtga agacgtcccc cttcagcagg
    20641 tagtcgtgat ccgcctccag cgccgcgagc gcttcgtcca gactgcccgg taccttcttg
    20701 atcagcttgg cctcctccgg cggcagatcg tacaggttct tgtccagcgg atcgcccgga
    20761 tccagcttgt tctggatccc gtccagcccc gccatcagca tcgcgctgaa cgtcagatac
    20821 gggttgcagg aggggtccgg cggccggaac tcgacgcgct tggccttctc cgacgggctg
    20881 tacatcggga tccggcacgc cgccgaccgg ttgcgctggc tgtaggccag gttcaccggc
    20941 gcctcgtagc ccggcaccag ccgcttgtag gaattggtcg acggggccgc gaacgccaag
    21001 atcgacgggg catgcttgat cagcccgccg atgaagtgca gcgccagctc gctcaacccc
    21061 gcgtagctct tcccggcgaa cagattcttg ccgtccttcc acaccgaaat gtgcgtgtgc
    21121 atccccgaac cattgtcgcc gtagagcggc ttgggcatga aggtgaccgt cttgccgtac
    21181 ttgcgcgccg tgttcttgat gatgtacttg aacagcagca gacaatcggc ctggtgcgtc
    21241 agcggggcga agcgcatgtc gatctccgcc tggccgccgg tcgccacctc gtgatgctgc
    21301 gcctcgatcg ggatcccgca gtcgatcatc gtcagcatca tctcggtgcg cagatcctgg
    21361 aagtgatcgg ccggcgggca cgggaagtag ccttccttat agcgcggctt gtagcccagg
    21421 ttcggcccct cgtcgcggcc ggtgttccag cgcccttcga tcgagtcgac caggtagaac
    21481 gactggttgg gctgcgagtc gaaccgcacg tcgtcaaaga tgaagaattc cgcctccggc
    21541 ccgatgtagc aggtatcgcc gatccccgtc gatttgagat acgcctcggc cttcagcgcg
    21601 atgttgcgcg ggtcgcgcgt atacttttcc atcgtcccgg gatggaccac gttgcagacc
    21661 agcgacagag tcggcgcctg gcaaaacggg tcgatcagcg ccgtcatcgg atcgggaatc
    21721 agcagcatgt ccgattcatg aatcgcctgc cagccccgga tcgacgaccc atcgaagccg
    21781 acgccatcgc tgaacgtctc ttcctccagc atccacgccg gctggctgaa gtgctgccac
    21841 agacctggaa agtccatgaa cttgtaatcg acgaccttga tccccttctc cttgaccacc
    21901 tggatcacgt ccttgggggt cttgcaatct cgggcttcca tacgtgacat tcctcccacg
    21961 gattttgggt acaactgggg ccaccggccg tatcccccga tacgcagcga ccgttggttg
    22021 ccagccggca gatgcaggat tcatgccgga accggattta ccccgatgcg acctcgggcg
    22081 cttgcccccg ccgtgccacg cggacaagcc ccggtccaac cgcggcgacc caccgcgccg
    22141 ccttcggggt gttatccgtt tccgttcaac attcgcatcc gtccttccag tttgcaatga
    22201 tgaagacgcc ccggccccgc ggacggggtg tcgggccatg gctgaccctg aacggccccg
    22261 gccctgccag accgttcacc gtgtcagtcc cgtcagggac ggctgggggg ctgccccttc
    22321 cccggggctg gtcatcggtc accaggccag tcccgtcagg gacggcagac ttcagcttgg
    22381 agacaatgac aagcaattgc ataaaccggc ggttcgatac cggggatgaa atcgaaggcg
    22441 tcaatgacca ggtcgaacgg ccgcgccctt gccggaccga tcaccatgcc ggtccgatca
    22501 ggtgtgtaac cggttcgtac ggccaagatt tcgtctgact tgctccactg ccagtctcgt
    22561 aaggacggca ggactgatcg acaggtcgga cgaccagcgc agggcaccaa gccagtcccg
    22621 tcagggacgg cagacttcag cccgccacgt cagtggcggg aaacgggaag cgaaaaacca
    22681 aagagtcccg tagggacgac agaaccttcc gatcgatcgc aaagctacga ggagcatact
    22741 tgattggcgg gatcaggcaa attgtctgaa tttctttatt cgaatttgtt tcgaatttcg
    22801 aattcggatt tcggatttcg gatttccgat cacgaggacg agcgacgagc gacggattct
    22861 tgagtgaggg ctggacatgc tccttgacga cttgacccgc ctggcgtgcc ccgcctgcgg
    22921 cggcgcgctg cgcgtcgacg acgcccacgc ccgcttcgcc gacgacctcc ctcccggcgt
    22981 cgatcccgcc gcgatggcct gggaaggcgc cctcgcctgc ctcgcctgcc acagctccta
    23041 tccgctcctc gaaggggtcg cctgcctcgc ccggctcgac tcgcgctggc tcatcccgct
    23101 caaggagatg gacagccgcc tctcgctctc gctgcgcacg atcgagcagc atccctggga
    23161 ggcgggccgc gcccaggcct acgccgagca ggagcggatc gtctgggacg agatggccgc
    23221 gctcttcgcc gccgcgctgg acgagctcga tctgcagccc ggcgtcaccg tgctcgacgt
    23281 gggcgccggc gaagcctgga ccacccagga attccagcgc cgcggcgcgc gcgtcgtcgc
    23341 cctggactgc gatctcgccc aactgcgcta cctctcgttt cacgacatcg aagggagcgc
    23401 cccctcctgg cgcgacgccc cctacatcca ccccggctcg ggcgcgcgct ttttctggaa
    23461 ggaccccaag ccgcgcgacc gctatttcac ccgtgtcttc ggcgacatcg gcacgctgcc
    23521 gttcccctcg gccagcttcg atatcgtctt ctgccgttcg gtgctgcatc acctcgccga
    23581 ccacgaacgc acgctgcgcg agatggcgcg cgtggtcaag cccggcggcc gcatcgtcgt
    23641 ctgcgccgag ccgatccgct cgatcctgac cgacgaggag gattgctacg accaatgtgt
    23701 tcagcgcgag gaagggatga acgagcgggt ctggcccgcc gcgcgcttcc tcgatccggt
    23761 ccggccctat gccgatcatc tgctggtcaa ggtgtggccg aatccccccg cgcgcagcct
    23821 gcgccgccgc ctgccgctga tcgccccgct gctggcgcgg cggatcgggc cgggcgcgcg
    23881 ctttcgcgga ccgaagctcg cgctgctcaa ctggatcgac gcctccatca acctgctggg
    23941 tcggcgcaac gacgcgccgc tgccgacggt ctgggccggc ccgccctcgc ccgaggacca
    24001 ggccgccttg cacgaactgg tggaggtgtt ccgcgtgcgg ccgggtgaaa tcgaagccgg
    24061 cgtggaggat ctgccgcgca agcgcgaagt cgtcggccgg ctgcggcgcg agctcctcgc
    24121 gcgcggcccg cagttgcccc gggccatcga cctcgcccgc gccgccgtcc cccagctcga
    24181 ccgcggctgg ttggatgtcg tgacgcacga cggccaaccc ggtcgccgga tggcgcgcga
    24241 ggccgccgtc acgctctcgg cgcgcaccga ggccggccag ccctgcacaa cgctgcgcgt
    24301 tcgcgccgcc cgccacggac cggtcaccgc gcggctggtg atccacgcca acggccggcg
    24361 cgcgctgccg atcgagctga acggacccag ttgggtcgag gcgcgcgtcc cgctcccctt
    24421 cacccgggaa ccggtcgtga cgctgcgcct gcgcgccgaa cacgcctcgg ccggctatta
    24481 cggcgcgccc ggcgccgaag gcgccccgcc cttcggcgcc gaaccggtca tcgccgtcgg
    24541 cgagctcgcg ctcgagtaac ccgtcgaccc cgcccgccgc accgcctcag gcgcccgcct
    24601 cgtccccgtc ggcggccgcc ggcagcgtca ccacgaacga cgcccccccg tccgggcagt
    24661 tgcgggcctc gatctgcccc ccatgcgcca gcacgatctg catcacgatc ggcagcccca
    24721 gccccgtccc ccgcggtttg gtcgaataga acagctcgaa cagatgctcc gcatcctcct
    24781 cgcggattcc cggcccgtga tcgcgcacct cgatcgcgat ctggtggtcg ccgtgcatcc
    24841 cggttcggat gcggatcacg ccgcggctgt ccatcgcctg gtcggcgttc atgatcaggt
    24901 tcaggaacac ctgctgcatc tgatcggcat caatgtggac gtggggcagg ttgtgctcgt
    24961 aatgcgtttc gatcgtcagc ccctcgcgcg ccagcgtctg gtaggcaatg atcgtctgcg
    25021 tcagcacctt gtggatgctc tggcgcttca tgtcgggggg cttggggcgc gagaacttca
    25081 gaaatgcctg aatccggtca tcgagccggc gggtttcgtc ccggatgaac tcggccgcct
    25141 ggcgtttctg ttcgtcgggc cggttcggat tgatcaggat ctccgccgaa ctgtgaatga
    25201 tccccagcgg gttcttgatc tcatgcgcca cggccgacgc cagttcgccc agcgtcgcca
    25261 gctggcgcgt cacacggagc tgctcctcaa gctggtggcg ctgcgtcacg tcgcgcatga
    25321 tccccagcac cagcgcgatt cgcccctgac tgtcgtacat cggggcgtag gtcacctcca
    25381 cccagaaggt ctcgccggtg cgcgagacga gcgggcgctc gccgcgcggg acgctctcct
    25441 gcgcgcgcgc gcaaaaaaac ccgccgctct cgccggcgtc gctgaaccgg ctgcatgccg
    25501 ggtgcgcgca gcggaacagc ggcgactcca gcccctggcc cgccagcacc tcctcggccc
    25561 gccagccggt cagccgctcg cacgcctcat tgaacacgcg caggcggcat tcgctgtcga
    25621 ccgcgaaaat cccgtcggtg gaatgctgca ggagcgcctg caggttcagc tcgctcttgc
    25681 ggtgcgaagt aacatcgtag aaataccagg cctcccagcc gatgctgcgt cccgcccccg
    25741 gtttgagcgc gcagatcacc agttgcacgt aggtctgcgg ccgcccgccc cacgtgaccg
    25801 ccagatcggc gatctcgacc cgccccttgg gcgaatgatg cagccgccgc agcgccgccc
    25861 ggatccgcga ccagccgtcc gacccctgga acagcaaccg gaacaggtcc tgcgcccgct
    25921 tgccctgcca ggccgccggc gagtcggcct gcaggtagaa atcgaccagc accgagttgc
    25981 accagcgcag gatgccgcga ctgtcgacca gcgcgattcc gtgatcgaac cgctcgcaca
    26041 gctcttcaaa tcgttcgcgc gacggttccc cttcgcgtcg cgctgcgggt tgcgggttct
    26101 tcgttcgcgc cacgggcgat ttcatcctgg gggcgaggga ttcggaacgg accggcggga
    26161 taaaccgaac gtaagcgagc tgaccgtcgc cagcggcgga cgtggaatcg ccggccgcac
    26221 cgggcggcga ggcgccgggg acgccgggcg ggggcgcacc gtcggcccgg ccggccagcg
    26281 cgcaatcgcc catcctatcg cgcgccattc cccgtcgcca acctaaagct gccggccctg
    26341 tcagcatcgg tatcggtatc gctatcggcc tcggcttcgc gatcgtctcg tgctcgccct
    26401 gatccgcggc gaggtcttcc aggcgcccct cgcgaccagc ttcagcgaga acgactgggc
    26461 gatctgccgg atccggctgc ccgccgggct ccccaccgcc gccgcttacg cgatccgcgg
    26521 ccacaacgcc gcccagccgg accaggcggg ctggacgccc cgcttcacga tccacgcgcg
    26581 caacgccgcc cagcccaggg cgtggccgga gatggaatga gtaggcgaag ggttcggccc
    26641 taccggacga tctgttcgaa atgccggcgg ttgcgcacca gcctccgcgc catctcgtcc
    26701 tgcagcgtcg tcccctcggc cagcaactcc ccccggcagg cccgctccag cgctgccagc
    26761 ttctcgcccc ccgaggggat gatcgtcttg tcgctgcccg acagcagccg cgcgcgcagc
    26821 tccacccgcc ggcaccagat gtaatcgcgc gcgatcgcgt cgacctgccc ggcttccagc
    26881 agaccctgct cgcgcgcgta atccagcacc cgccgcgtgc cgaggcccag cagcgccgga
    26941 tgatcgcgca ggtgcgtgag ctggaggtgc tgcgcgatga actccagatc catcagcccg
    27001 ccgcggccct tcttgaaatc ggcgaagacc cagcgcggga gattcctgac cgaatcctcg
    27061 atccgccggc gcatcgaccg gatctcctcg ccgatgcgca gctccggcaa tcgtcgcagc
    27121 acgcgctcgc ggaccgtttc ggtcagccgc gcccccagcg cgcgatcgcc cgccacccag
    27181 cgcagcttca tcgccgtctg cagttcccac ggctgggcgc gctgcgcgta gtattcctcg
    27241 tagctcgtca ggtgcggcgc cagcggcgcg ccggaaccct ccggccgcag ccgcgcgtcg
    27301 atcgcatagg cccgcccctc ggccgaaacg tgcgtcagga agaacagcac gcgctcggcg
    27361 agcgcggccg ccgcctccgc cgccgatccg gcgcccgccc cgaacggctc ctcccagacg
    27421 aagatcagat ccaggtccga ggcgaacccc agttcgcgcc cgcccagctt gcccagcgcc
    27481 atgacggcga acccggccgg caacggcggc aacccgcgct ccgccgcggc gcgcgccagc
    27541 gcctgctcca gcacccgctc cgccgtcgcg accagccgct cgcagtaggc ggtgtagtcc
    27601 agcagccccg ccagttcgcc gaggctcgcc atcaggtatt caaaccggcg gaagtaggcc
    27661 agcgcctggc agaacgcctc ctcatcccgg gcgccctgaa gccaccggtt caaccgctgg
    27721 cccatggcgc cgggatccgc cggcccggca atcagcgaca ggtcgctcag gtaatcgacg
    27781 aattcgggat gcgcgatgag cgtctgcgcc agcgcctcgc ccatgccgaa ggccttgagc
    27841 agcaattcca gcagcggctg cgcgccggcg aaggtctcgt agaacaccgc gcgcgcgccg
    27901 cacgcctcgg tgaacgattc gaaatgcgcc agcgccacgc gtggttgcgg cgcgcgctgc
    27961 aggcagcgca gcaggatcgg taacaggcgt tcgaactgct gttgctcgct gacgctcaga
    28021 taaaccgtcc gccccatccc ggccagccgg cgcagcgtct tcagcgcgct gccgtcggcc
    28081 agctcgaacg gctgcagggc cggcagcacg ttgccctcgg gctcggccga gagcaccgcg
    28141 cgcgtcgccc gctcggcctc atcggccggc gccggcgcgg gccgcgcctc cgcgccctgc
    28201 gggagaaaaa acttcgcaaa tgccgcatgg acccgctcgg tggtctcgcg ccacgccgcc
    28261 tgaagctgtt cgcgaggcgt cgcccccggc cgcggcgtca gcccgcagcg gcgggccagc
    28321 gcttccagtt cccttggatt gtccggcaag cgatgcgtct ggcgcagttg ctccatctgc
    28381 aggcggtgct ccagcgtgcg caggaagatg tagtcgcttc gcagccgctc cgcctcctcg
    28441 cgcggcatca ggccctcgcg cgccagcgcc gccagcgcct ccagcgtgct gggtgaatcg
    28501 aggcccggcc gggcgccgcc gtgcaggagc tgctgcgtct gcaccagaaa ttcgatctcg
    28561 cggatccccc cccggccccg cttgatctcg cggccggcat cgccgcgacg cgccacttcg
    28621 ccgtcgatca gccccttcag ttcatacacc tggccgacca gcccctccgc gtgcagcggg
    28681 ttgaacacaa acggccggcg caacgcctca aacccccgcg cgacccgctc gtcgcccgcc
    28741 accggccgcg ccttgagcag cgccagccgt tcccacgtgc gcgcctgcgt cgcgtaatag
    28801 atctcgcacg ccgccagcga ccgcgtcagc gggccgctct ccccctccgg gcgcagccgc
    28861 gtgtcgaccc ggtagaggta gccctcgggg ccgtgctccg tcagccagct cagcatcgcc
    28921 tcggccaggc gcgtgaagaa ctggtggttg gagatcgccg gcgcgccgtc ggcccccccc
    28981 gtcgttgtgc cctcggccga ataaacgaag atgacgtcga tgtcggacga aaaattcagc
    29041 tcgcccccgc ccagtttccc catgccgatc acgcagaagc ccgccgcggt gggcgcggcg
    29101 cccggctcgg cgtcgtccat cggcgtcccg tggcgttcct cgagcgggcc atacgcctcg
    29161 cgcaacgcca ctcgcagcgt ggccgccgcc agttcgctca gctcgcccgc catctgcatt
    29221 tcgtcgctca gcccgatcat ccggcgcacg cccaggcgca acagctcgcg gcgctggcag
    29281 cggatcgccg catcgcgccg cgcggccggc cgccggaacg gccccagcgc cagctcatat
    29341 tggcgcaggc agcgctccag cgtgcgtggc tgccccagct ccggatcctc ttcgagccat
    29401 tcgaaaaact gggggtagcg gatcagcaga tcggccagga attgtgagaa accgaagagg
    29461 cggcagagaa tcgcgagcgt cggcgccggc accgtcgcca gcagcgcgcg ttgttcacgc
    29521 ccgaaccgcg cctcgccgta gcgcagcaga tcgagcagcg cctggttcgc gtcggccgcc
    29581 gccgccagcg ccggcgtcaa tccgcgcagc agttcccggc gcggcgcctc gggcagttcg
    29641 gcgcacagcg cgtccagatg ccgccaggcg tcgcccggct cggcaaaccc gagcgccccc
    29701 agcagccggc gcccctcctc ctcgcgtccc gcggtcaggg cctcggtcag cggctcggcg
    29761 agcgcttgcg ggtcggcggg ttcggatcgg aagggcgcgg cgctcaccgt cggctgctcg
    29821 gctggactcc ccgaaggggc gatgcccgtt cagtttggag gggaacgccc ggcggaaaca
    29881 agcgcaaaga cgcccgccct tctccagcat cgctatcggt atcggtatcg caatcgatcc
    29941 ccatcccgat tccgttgcca cggggggata atcaattgac gctcgcgcga accggttgcg
    30001 ataatcaggg gatttgcggc cccctcccca aggcccgagc gattccgcgc gttcacaggc
    30061 tgtgcttcat gctcaatttc atttttcggt ggctggtcgg cgataaacgg cagcgcaatt
    30121 tcaagcaggt tgcgccgatt attgcccaga tcaacgactg gtgcgagcgc tacaacagcc
    30181 tgaccgacga cgaactcaag gccaaaacga ccgaattccg agagcggctg gcccagggcg
    30241 ccacgctcga cgacctgctg cccgaagcct acggcgccgt caagcaggtc tgccgccggc
    30301 ttgtcggcca gagctgggaa gccgccggca tcccgatcaa ctggaacgat gtccccttcg
    30361 atgtgcagct ctgcggcgcg atctatctgc accgcggcaa cgtctgcgaa atggccaccg
    30421 gcgagggcaa aaccctcgtc gccacgatgc ccctctacct caatgcgctg gaaggcaagg
    30481 gcgcgcacct cgtcacgacc aacgatttcc tcgcccgctt cggcaaggag tgggtctcgc
    30541 ccgtctacga gcggctcggc atgaccaccg gcgtcatcct gagccagatg acccccgccg
    30601 agcgccgcga agcctacaat tgcgacatca cctacggcac gaacaacgaa ttcggcttcg
    30661 actacctgcg cgacaacatg gccctgcagc ccgaacacct ggtgcagcgc gagcatcact
    30721 tcgcgatcgt cgacgaggtc gacagcgtcc tgatcgacga ggcgcgcacc ccgctgatca
    30781 tctccggcca ggtcgaccgc tccacccacc agtttgatcg gctcaagccg ctcgtcgtcc
    30841 gcctggtcga aaagcagaac caactcgtca actccctggt cgccgaggcc gaacgcctgc
    30901 tggaggggac cgacccggac aaagtggacg accggcgcta cgaagccggc atgaagctgt
    30961 tgcaggcccg gctggggtcg cccaagcaca agcgcttcac caagattgcc agcgacggcg
    31021 cgatccgccg gctgatcgac cgggtcgaga acgacttcat gcgcgacaag cggatgcgcg
    31081 aactggagga agcgctcttt ttcgtcgtcg acgaacgcgg ccacacgatc gatctgaccg
    31141 agctcggccg ccagaccatc tcgcccgata atcccgatct gttcctgctg cccgacctgg
    31201 tcgaggaatt cgcccagctc gaaaatcagg ggctctccga ggaggagctc gaacgcaaaa
    31261 aagaagagct gatgaatcgc cacgaggagc gcaccgagga gctgcacaac atctcgcagc
    31321 tcctgcgcgc ctacgtcctc tatgaacgcg acgtcgatta cgtcgtgcag gacaacaagg
    31381 tggtgatcgt cgacgaattc accggccggc tgatgaccgg ccggcgctgg agcgacggcc
    31441 tgcatcaggc cgtcgaggcc aaggaagggg tcacgatcga gaaggagacc cagacgctcg
    31501 ccaccatcac cctgcagaac tacttccgca tgtacaagaa gctggcgggg atgaccggca
    31561 cggccgagac cgaggccgag gaattcgccc acacctacaa gatggacgtg tatgtcatcc
    31621 cgacgaacgt gccgatccgg cggctcgatc tgaatgacct gatctatcgc accgaacgcg
    31681 aaaaattcaa cgcgctggtg caggaagtgc accgcctgca cgaatacgga ctgccggtgc
    31741 tcgtcggcac gacctcggtc aacacgagcg agaagctctc gatcatgctg cggcgccaga
    31801 agatcagcca cagcgtcctg aacgccaagc agcacgagcg cgaggcacag atcatccgcc
    31861 aggccgggca gttgggtgcc gtcacgatcg cgacgaacat ggccggccgc ggcaccgaca
    31921 tcaagctggg ccagggcgtc atcaagtgcg ccgacggcgc caagccgccg gggggtaagt
    31981 actgcgtcgc ctgccccaac cgcccgcccg gcgccgaggt cgaccccgac atgcccccct
    32041 gcggcctgca gatcctcggc tcggagcgcc acgaggcgcg ccgcatcgac cggcagttgc
    32101 gcggccgcgc cggccgccag ggcgatcccg gctcctcgcg cttcttcgtc tcgctcgaag
    32161 acgaactgat gcgcctgttc gcctccgaac ggatggccaa gctgatgtcg aaggggttcg
    32221 tcgaggggga ggcgatgagc catcgcatcg ccacgcgcgc cctcgaatcg gcccagaaaa
    32281 agatcgaatc gatcaacttc gagcagcgca agcgcaccct tgactacgac aacgtcatga
    32341 acaagcagcg cgagcggatc tacggcatcc gccgcgaggc gctgctcggc cagggcagca
    32401 atctcgaacg cgtcctggaa ttcaccggcg aagcgctcgc cgacgcctgg gaaaaacact
    32461 gccccggcaa cgacgaatcg gaatgggacg tcacgggttt cctcgactgg gtccgccgct
    32521 acgtcccgct gatcgacctg aacaacctgc ccccactgag cgtggactcc gtcgacgatt
    32581 acctcagcga aatcctcgaa cgctgcgccg ccgtctaccg ccgcaagacc gagatgatgg
    32641 gcgagcagat gaccggcgcg ctcgcccgct atgtcctgct gacgatcatc gacacgcact
    32701 ggcgcgatca cctgctcgcg atcgacgaat tgcgccacgg catcgccctg cgcagctacg
    32761 gccagaagga cccgctgacc gaatatcagc gcgaggcgac cctgctgttc gaggagatga
    32821 tggccgccgt catgcgcgag atcttcgagc gcatctatcg ggtctatgtc gtccaggagc
    32881 cgacgaccgg cacggcacgg ctgacctacc tgaaggagga gggtcgccag agcatcaccg
    32941 acgccgcccg ctccgccgcg gcgcaggccg cccaggccgc cgaaggccag gcgcaacagg
    33001 ggcagaccag cgagggcgag cggccggcgc ggcccaggac ctaccgccgc gaacagccca
    33061 agaccaaacc caatgacccg tgctggtgcg gcagcggaaa aaaatacaag aaatgccacg
    33121 gcaaccccgc cctgcagcag gcccccctcg ccagccagcc ggccggtccg acggatgatg
    33181 acgccgcctg accccggctt gacgctgctt cacttcggcg atctgcacct gtggcggatc
    33241 ggtctggatg ccgacccgcg attcaaacgc ctgctcggcc tggccaacct gcttctccgc
    33301 cgcggccggc tctttccgct ctccgtcgcg cgccttgtgc tgcgccggat cgagcacgac
    33361 cccgccgatt acctctgctt ctccggcgat ctgacgacca cttcgctcaa ggccgagttc
    33421 gaggacgccc gccgcctcct cctcccgctg ctcgacaagt ggcaggcccg cttcctcgcc
    33481 atccccggca accatgatcg ctacaccgtg cgcgccgccg gccggcgcta ctacgaaacg
    33541 ctcttcgccg gcgagcacgc gcgttttccg ttcgaggtcg acctggacgc cgtctggacg
    33601 ctgatcggca ttgactgctc cgtgccgcgc cggttcacct cgcgcggccg gtttacgcgc
    33661 gaagcccgcg acgccctcgc gccgcgcctg gccgaacacc gccggcgcgg tcggcgcctg
    33721 atcgtcatgg gacactaccc gctgctctac ccggcgcgcg tccccgtcca gctcgaccat
    33781 gccctccccg agcggatggc cctgttcgag ctcctcgccg aacatcgcgt cgacgcctac
    33841 ctgcacggcc acaagcacca gcgctggcgt ctgaagcacg agggcgtcac gctgctcaac
    33901 tgcggttcgg ccggcatgga tcacccccgc catcgcccgg ggtatcttcg catccagctc
    33961 actcacgacg ggatcggcgc ggtccagtcg gtctcgctgc gtcaccgcgc cgacatcgtg
    34021 cgcgaggcgc ggcgcgaggc ccgcgcgcac gaggcggccc acgaccacgc cccccccgac
    34081 acaggcgaac tgcccgccgg cgaatgggtc acgcgactgc ttgaggagga tggcggcgcc
    34141 gtgcattgaa ccgcgcggcc gccgccccac ggcgggtgct cagggcgcgg cgccgaccgg
    34201 cgtcgcgcgc ggcgtcgtca gcaactcgcg catccgcggc aggttcagcc gcgacggcag
    34261 aatcgtgact tcgggaatca gataatacgg catctcgttg ctcaggaact gcgccagctt
    34321 cgtcgccgag tccagataga gataaaccgg atgatcggcg gcggggaaat tgggctgctg
    34381 cgcccgccac tgcttgaccg gctcggccac gtcgggcggc gaccagtcct cctccagcag
    34441 aatccccagc gtcgggtgaa acgccaccag gtcaaatgtg atgttgttga tctgaaccgg
    34501 cggattgatt tcaaaccgcc cttcgcggtc gaccgacgtc cccagccgct ggtaatcctc
    34561 cggactgcag accaggttga tccggaacgt cagttcaaaa ggactttcct cgttgaaccg
    34621 gacggccagc gggcggctca gaccctgcgc gatcgccagc ggaatcgccg ccgtcgtcgg
    34681 cagatcggcc ccattgacct gcagcgtcgg cttcaaatgc cacggcgcca gcggcagttg
    34741 ctggctgagc gtccgcgcgc cgcgccgttg ctggcccaga tccgaaccga agttcatcac
    34801 cagcgccgcc acgtcggcca gccgccagtc gccgcggctg ccggtcgccg tgtccatctg
    34861 cggcgccttg atcgtcgcca ggcgcgatcc gaccgcctgg ccgatctcct cgaccagttg
    34921 caggcgattg gcgaatacct tttcgtaaaa ctccgtcgct tcggccagat tgccctgctg
    34981 ctcctgccgc gctccctccg ccagcagttt gagggcgttg agctgcggcg tcaggctcac
    35041 gtcgcgcggg aaccgggcgg tcgcgacttc cagcgccgcg atcgcggcat cgtaatggcc
    35101 cgccccggtg ttcgcccgcg cgatcagcag agccatctcc ggcgagaccg agggatccgc
    35161 ccacccctgc aatttcccca ccgccagccc gtattccccg gcattgaggt tgatctgcac
    35221 ctgttccgcc agcacttggt cgaacggtcg ttccagcagc tcgcgcgtcg ccgcgtcgat
    35281 ccccggcgca ttcttccatt ccggatgcag caggaacgcc tcgttgagct tcgacaccag
    35341 ttgcgcgtaa tgcggtgcgc ggacgtcatc cttcgtcgtc aggaacagct cgatcaggta
    35401 gcggtagacc tgcggcagca gcggaacgat cgggctgtcg ggccgcaggt tgtcgggcca
    35461 gaggatctgg ccgaaatact cgccggccag cagcaggtcc ggcttgccgc gcgccgtcag
    35521 gcgccggccg atcagctcat gcgcctcggg cagccccgga tcgatcagca gcgactggat
    35581 cagcaggtcg ttgcccaggt cgtcgtcgat ccggtcgtag gcctcctgcg cccgccgcag
    35641 caggtcgcgc gcgttctcac ggcgctgcgc cagcgtcccg ccgtggcgtt ccagttcggt
    35701 caactccgtg tccagccgca gccgctgcgc cagcagatgc tcatcggtca ccgtcagccg
    35761 gcgaacctgt tccagcagcg tccgcgccgt gctcagatcg ccgttctgga tcgcctcctg
    35821 ggcgcgctcg agcagttgcc gtgcgttgtc ctcaacatcc agccgttcga cgatcttgac
    35881 ccgttcgtgc gggatcggca ccaccacccc gcccgtatcc aggtagatgt tgtagacgtt
    35941 ttcgtcgacg atcaccccct gcagactctg gccctgcgtc gtgacgatct tgtcggcttg
    36001 gaccggctgg aacagcgccg gcagcagcgc ggcggccgcc aacgcggcga aacggcgaaa
    36061 acggggacaa tgcatcatgc ctggctttct ggcgcccgct ccggccgggt gcgccggggc
    36121 ggtcggccac cgatgggcgg agggaggaaa cggatttgaa acgagccgat acccgtgccg
    36181 cggaagggca tgcccgttcc ccgccggcgc ggatcgcgcg gtccgatggc atgctggcat
    36241 atcgccccgg tccatgcaag tccgcacttg ggaagactga gcgccacccc cgccccgggc
    36301 tcggccgggc ccgtctcaac ccgcccgggg atcggcctgt gcgaggattt gctcgaagat
    36361 cagaacagcc acccgctgac tgaccgtttg tgtcgcggac cacgcatgcc agcgcgaatc
    36421 cagccgcttg tactccgaaa gatcggtcag tgagtgcaaa ttcatgctat gcgcaaggga
    36481 ccggccgcgc aagtcggccc gtcggcgcat ccaccaggtt tcaactcccc ccgccggtca
    36541 gccgggatcg cgattgcatc ggctccccgc atggccgaca atggcgtcca cccctcaacg
    36601 ggacgggcaa gccgtggaga caacatctca gtcccctgag agtgcgcgca agtcgtgctg
    36661 cgccccggcg cgcggcgagg aaaacccggc gcttccggcc gccgaacgcc ccgtcgcccc
    36721 ggcgggcggg cccccgccct ccggcatggt gcggcttccg ggcggctcgt tcctgatggg
    36781 caccgactat ccggaaggat tcgccgaaga cggcgaagga ccagtccgcg aaatcaccct
    36841 gtcgccgttc tggatcgacc agacggccgt gaccaatgcg cagttcgccc ggttcgtcga
    36901 gccgaccggc tacaagaccg acgccgagcg ctacggctgg tcgttcgtct ttcacctgct
    36961 gttgccgcct ggcacgctca agcgggtcac gctgcgcgtg cagggctgcg agtggtggtg
    37021 ccgggtccgc ggcgcctgct ggcggcaccc cgaggggatc ggcacctcga tcgcccaccg
    37081 gctggaccac cccgtcgtgc acgtctcgtg gcgcgacgcc gccgcctatg ccgcatgggc
    37141 cggcaagcgc ctgccgaccg aggccgaatt cgaatacgcg gcgcggggcg gcctggtcca
    37201 gaagctctat ccgtggggcg acgacctgac ccccggcggc gagcaccgct gcaacatctg
    37261 gcagggggaa ttcccgcgca taaacacggc ggaagacggc tacgtcggca ccgccccggt
    37321 gacggcctat ccgcccaacg gcttcggcct gtatcagatg accggcaacg tctgggagtg
    37381 gtgctgggac gagtggagcc gcgatgacca tgcgcggccg gaggcgccgc gcgtcaaccc
    37441 ggccgggccg ccgccgtctc ccgaccaccc ccgcaaagtc atgcgcggcg ggtcgtatct
    37501 gtgccactgg acctactgca atcggtatcg catcgccgcc cgcacggcca acacgcccga
    37561 cagttccacc ggcaacatcg gtttccgctg cgtaatggat ctctcaacct gattctcaat
    37621 cggtatcggt atcggaatcg ctatcgcaat cggaatcgcg ttgtgggacc ggcgccctcg
    37681 ccggtcgatc ccccggctcc acgtccttca aggccccccc catgccctac cccaactcag
    37741 ccgcccagcg cccagcccga aacgcggccg gatcaaaccg gtagcgccca tccgcatcgc
    37801 ggaacggccg ccactcggcg ccgtcgaaaa cctcattcgg tcggaattcc agcttgtaac
    37861 gcatcgacgg gtttccgtgg atcgcgtagc cgagataata gtacggaatc ccccgctcgc
    37921 gcgcagactc gatctcacgc agcgccgaca gcgtgccggg cgacagcggc gccaactccg
    37981 gatcgaaaca ggcatagatc gacgacgcgc agcgcgcggt caggtcgagc a
//
LOCUS       DAOPCO010000009        36743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1292_length_36743_cov_5.503789, whole genome shotgun sequence.
ACCESSION   DAOPCO010000009 DAOPCO010000000
VERSION     DAOPCO010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 36743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36743
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1292_length_36743_cov_5.503789"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..2257
                     /gene="fusA"
                     /locus_tag="PLS90_01515"
     CDS             179..2257
                     /gene="fusA"
                     /locus_tag="PLS90_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPK01111.1"
                     /translation="MARKFPLDKIRNIGIMAHIDAGKTTTTERILFYTGRSHKLGEVH
                     EGTATMDWMAQEQERGITITSAATQCYWQDHCINIIDTPGHVDFTVEVERSIRVLDGA
                     IALFCAVGGVEPQSETVWRQADRYKTPRLAFVNKMDRVGADFFNCVQMMRDRLNAHPV
                     PIQIPIGAEDQFEGIIDLVEMVARVWPLGADEERGRVFQDVPIPENLRDLANEWREKM
                     LEALAEYDDDFAEAYLEEKAFEPKELHDHLRKATIACHITPVLCGTAFKNKGIQLLLN
                     AVLRYLPSPKDVPAVHGQLPHEPDTVVSREPSEDAPFAALAFKIMSDPHVGRLTYLRV
                     YSGKVKAGSYVYNVTRDQNERLGRLMLMHANDREQIDEVRAGDIVAVIGFKNTTTGDT
                     LCDPEHPIILEAMSFPEPVISIAIEPKTRNDQDKMGSALQKLAEEDPSFHVKVDKETN
                     QTIISGMGELHLDILVDRMKREFKVEANVGAPQVAYRETITKTVEVEGRFVRQSGGRG
                     QFGHCWLRLEPVEPGSGFEFVDEVVGGVIPREYIPAVEKGVIEAMNNGPIAGFPVVDV
                     KVTVYDGSFHPVDSSEMAFLIAGSMAFKEGAKKAAPNLLEPIMKLEVTTPEDYLGAVV
                     GNLQQRRAHIDEMRARGSIQVISASAPLGEMFGYATELRSQTQGRASYSMQFSHYAPA
                     PKSIVEKITS"
     gene            complement(2272..3741)
                     /gene="pepD"
                     /locus_tag="PLS90_01520"
     CDS             complement(2272..3741)
                     /gene="pepD"
                     /locus_tag="PLS90_01520"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HPK01112.1"
                     /translation="MPASSDRVLKHFQAISRIPRPSGREEAVMRMLEDWAGGHGFAVR
                     RDARGNRVIAVPASPGMAARATVVLQGHVDMVCEKTPESAQNFLTDPISLRQERDEDG
                     EEWLSATDTTLGADNGIAVAMMMALAEDPAAIHPPLELLLTVEEETGLIGAGALEPSI
                     VSGRILLNLDSEEEGVFTIGCAGGRSTSIRLPLAREAEPGPGELYALRVGGLRGGHSG
                     VDIHLGRANALKLLAEAWSALAGAAPARLVSVDGGSADNAIPRDARAVVRLVADRLND
                     ARHVIATQQERMRREYGDRDPETTLSLEPVSQEVTGHPLTADCAERLRVLLGELPHGP
                     QTMSREFAEVVETSCNLARVETRPDALRIITSQRSSSREDMQSLTERIHRLARSAGAE
                     AADDGRGYPPWPPRRDAWLNGLCREVWRELSGAEPEINVIHAGLECGVIGDRIAGMEM
                     ISLGPTIRDPHSPRERVSLGSVGRTYDFLVKLLARLAQG"
     gene            complement(3815..4246)
                     /locus_tag="PLS90_01525"
     CDS             complement(3815..4246)
                     /locus_tag="PLS90_01525"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPK01113.1"
                     /translation="MPQPEPVSPADLDRLFAAAREAAVAAYAPYSHFAVGAAVLDDQG
                     RLFAGCNVENVSFGLTICAERHAVGAAVLAGARKLLAAAVYTPTAVPTSPCGACRQVL
                     AEFGPTMTVYLFCDGTAPRRHALRDLLPESFGAGESGFSAG"
     gene            complement(4369..5451)
                     /gene="leuB"
                     /locus_tag="PLS90_01530"
     CDS             complement(4369..5451)
                     /gene="leuB"
                     /locus_tag="PLS90_01530"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPK01114.1"
                     /translation="MTDRVLKVAVLAGDGIGQEVIPETLKVLEAATRGRGVRFEWREC
                     LVGGASIDAHGLPLTDEVLETCRRSDAILLGAAGGPKWDDQPLALRPEKAVLSIRKTF
                     GLFANLRPIWLPECLRHRTPLRPDLLEGGVDMLIVRELNFGLYFGERGRRPGPEGDIE
                     AYDTCVYSEKAVEQVARVAFDAAGRRRKRLMSLDKSNVLETSRLWREVVIRVGADYPD
                     IELRHQLIDSATMVVVQRPTELDVILANNEFGDIISDEASVLGGSLGMIPSAAIGPRP
                     PFFYEPISGSAPDIAGQGIANPIGAILSGALMLRYSFEMEEAAAAIEQAVRRALEAGH
                     RTRDIADRNEKSIGTQEMTRRIIEAL"
     gene            complement(5521..6747)
                     /locus_tag="PLS90_01535"
     CDS             complement(5521..6747)
                     /locus_tag="PLS90_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPK01115.1"
                     /translation="MADPLHLKRLRDSVDKSTGRKDWLLFKLRAKWIDLSTADLSALK
                     LRDYDLSELNMSTAIFRGAELINCDLSDSILSFSDFRRANLRNSCFDRADLRSANLQE
                     AEMVDTCLEGANLSQARLSGCNLVGADLSGSNLTEADLRGAVLKFARLTGAVVDGADV
                     TDADLTGAVLDDEAPAQMLNFQSAIIDDRKYREMRCRLIGEKRPRVIADPDQILRGEQ
                     PAAPTEEEEDRIEHTHQIPLRAVEPDLTTAEGCCAVLEIEPGAGHEEITRAFRRKAKL
                     FHPDKVRHLRPRLQELAGEEFMRLRKAYEFLTRRATTRKIARNIHWPPGMERNRSPYD
                     YSIGEYEALAALNGDNADILYNLAWKYFEAGRMAEAWRGFERVLSINPNDDDAHYNLM
                     IIRLYRELLQIPVELT"
     gene            6921..8051
                     /gene="obgE"
                     /locus_tag="PLS90_01540"
     CDS             6921..8051
                     /gene="obgE"
                     /locus_tag="PLS90_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPK01116.1"
                     /translation="MSQSFVDSVRVYVKGGDGGNGAISFRREKYVPHGGPDGGDGGPG
                     GNVWFVASTELATLIDLKLRPSLKAGAGGHGLGKNMTGRAGDDITVEVPLGTVVLDED
                     GNELADLVEAGQRFLAAAGGAGGAGNQHYATPTNQAPRKAKRGQPGQERSLQLELKLI
                     AQGGLIGLPNAGKSTLLASLTSATPRIAPFPFTTLHPNLGVMEVDVARRVTLADIPGL
                     IEGAWRGAGLGDRFLRHIERTALLIHLVAPPEDCQLLPDLRPEDEAEMAELGRDGVRA
                     AYQLVRRELEAYSEELSRKPEIIVLTKIDLLPERLRQAWLAVLRETGKPVAAISAETG
                     EGLDRLKALLIEQLDRMGMIWDDPRRPHLAAAPPVEPDRSGD"
     gene            8132..9340
                     /gene="proB"
                     /locus_tag="PLS90_01545"
     CDS             8132..9340
                     /gene="proB"
                     /locus_tag="PLS90_01545"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPK01117.1"
                     /translation="MRPTRDVQMIHIDNIQGTGRGIDLRKVVIKLGSAVVAGEGHQLD
                     LVTLGALADSVANLRRQGVDVILVSSGAIGMGRRAFPDFTARTIPDRQALAAIGQVGL
                     MHAYKTLFNARDLRAAQILLTRDDMEDRRRYLNARYTLERLLELGAVPVINENDTVTI
                     DELQFGDNDELSAVVAIKMGADMLIILSIVDGLHEAEADPTDAGARGRKRKGRHAAGR
                     RIDVVESVDDVLGLADRTRSAMGTGGMQSKLKALHMASQAGVHAVIAPGKRAGIVEEI
                     VSGRFPGTYFVPRVGHRLRGKPRWLAFGRRATGRCIVIDAGAVEALRVGKKSLLAAGV
                     RAVRGQFERGDLVEVHDEAGARVAKGLTNYSAEELDRIKGKRTPQIREILGELDYPEV
                     IHRDNLAVVD"
     gene            complement(9423..10379)
                     /locus_tag="PLS90_01550"
     CDS             complement(9423..10379)
                     /locus_tag="PLS90_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01118.1"
                     /translation="MLTTITRPFLAALFGMGLVLPATAMPPAQDLNGSKPLVGTERIV
                     FETGPSGEWHQVEGSSKVDTDRGYAVEREPIRSREPVVLGLPASSTLSDLSEPEKERI
                     IKENEELTTREEIRAREEGLTYDASPPSPANELYYPIDGHITRIMKVEYPGVSEEHIL
                     AEVDTAKSSQGRVELGPASKVAHLNLKEGDSIYVEGRPGRLNGQPVMIAQTVKANGEV
                     IKIEREMRTSLHQVDGSIEGLRTVRYQGQDGDHLIARVDLKGRGMETVDLGPADQLRD
                     LELSEGDLIGLSVYPGRINGDAALIAWKVEKGDRSVEIERKR"
     gene            complement(10442..10996)
                     /locus_tag="PLS90_01555"
     CDS             complement(10442..10996)
                     /locus_tag="PLS90_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01119.1"
                     /translation="MRTMWKAWGLAAILALGAVPAAFAEEGPSGIDIRRDTGTRSDSS
                     GGTRPDRDPSRLDRDSFIVRDRDTHLRSDDPDRRGSRHDEPELGGEGTRAQRYFDRDR
                     DRDDDGARFDRSDRDWERRLRSDHRRGWVEHYEFYTRDYDRYDWYDNDDRWDRDSNWY
                     WEYRTRDRVNVRENAFWNQAATSR"
     gene            complement(11417..12595)
                     /locus_tag="PLS90_01560"
     CDS             complement(11417..12595)
                     /locus_tag="PLS90_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPK01120.1"
                     /translation="MAYRIESLYGVEDAPFRRAMGNLLGPPLVAGNRLTELCNGDQIF
                     PAMLAAIAGARETITFETYIYWSGEIGRRFAEALAERAHAGVRVHVILDWFGARIEST
                     YIDQMERAGVQIVKYHPLRWFNLSRLNYRTHRKLLVIDGRIGFTGGVGIADEWTGDAQ
                     DPDHWRDMHYQLEGPAVAQLQAAFMDNWIKTSDQVLHGDAYFKPAEPAGDACCQVFKS
                     SPNEGSSSVRLMYLFSLAAARRHVRIANSYFVPDEHAQQELVRARERGVAVDIILPGG
                     TIDYEAVRRASRANWGPLLEAGVRIWEYQPTMYHCKVMIVDDCWVSVGSTNLDNRSFR
                     LNDEANLNIHDREFAARQVEIFERDRELSREVSLAEWRRRPLREKALEHFLNLFRSQL
                     "
     gene            complement(12767..13726)
                     /locus_tag="PLS90_01565"
     CDS             complement(12767..13726)
                     /locus_tag="PLS90_01565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK01121.1"
                     /translation="MPPKYYMPEPTRPIQWFRHKYIEKLEPVPEVPKNVQIQTISGCN
                     ASCVFCPNKKTDLQIPFANRMDWDLYRSIIDQISEMGVRRISPYLMNEPMLDPEFPKR
                     VEYITRKKKPWQFSKINSHGGLLTERMAKGILDAGLDRVNFSVQGLDPVIYEDVMKLP
                     LQKTLDNIDRLLELKRAGNYKKPRIRVCMLMTKYIEPQMDKIQEYWGSRGVKININQL
                     ENRGKHKAIKSDEIAVRQLQNYDWCSRMFEQMYILYDGRMVMCCADWEQTGVMGDASK
                     KPLREIWHDEVYTRYRRNFLRGRVQGTICDGCTKDGAGDEYDD"
     gene            14274..16034
                     /locus_tag="PLS90_01570"
     CDS             14274..16034
                     /locus_tag="PLS90_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HPK01122.1"
                     /translation="MFILGIHDGHNAAACLLEDGRIVAALQEERLTRIKNHDVFPARA
                     VHWLLESRGLTLRDIHAVAMNGHHQPAHRDRARLIQDTRRGGDLHPARALRRLARKTP
                     LLALWRRRRRADREAEAHAAGIDPQRLAWIEHHRCHAEAAWWGCPWRDGPVLVLTCDG
                     AGDDLCATVNVANEDGRGGLRRIAQVHESHSPALIYLVVTTLLGMVPNEHEYKLMGMA
                     PYAGEQATEEVARLFERLLEWDPNQPLAWRRRPGVPNAYYSYDYLKAMLDLRRFDAIC
                     GGMQRWVERLLAEWVRRCVAATGLRRVALSGGIFMNVKANQVIAQVPEVDDLFIFPSC
                     GDETNTMGAAWAAYAGMAAPDAPRPEPLGPIYWGAAPAPAEIEAALAAQGAHFRWSRP
                     ADLAAHAAAFLERGEVVARYAGRAEFGARALGNRSILANPARPEVVRRINAMIKSRDF
                     WMPFACSLLAERADDYLVNPKRLRAPYMILTFDTTDRWADIAAGTHPSDRTVRPQIME
                     PAWNPEYHRLLKAFEARTGIGGLLNTSFNLHGYPIVNTPAEALDVMRRSGLNYLILGD
                     LWVEKLSDESRDGGRHAVAP"
     gene            16018..16800
                     /locus_tag="PLS90_01575"
     CDS             16018..16800
                     /locus_tag="PLS90_01575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01123.1"
                     /translation="MPSLREQPSIAAVERLMTRTGAVDITVVIPTLNEAGNIGELVRR
                     ARAMLDGMGVSHEILAIDGGSTDGTCEEAEQAGAVAIREGGHTYGAALRTGFAHARGE
                     FIITMDSDLSHEPEFFEQLWQARRRADIVIASRYVAGGKADMAPFRSVLSRILNFVYT
                     RILRIPVHDISSGFRLYRRAVMERTETTAHDFDVLEEVLIKLYVQGHAVAEVPFHYRP
                     RKHGRSHAKLVRFALAYLKTLWRMARLRYGTAGGRDARERRP"
     gene            16857..18047
                     /locus_tag="PLS90_01580"
     CDS             16857..18047
                     /locus_tag="PLS90_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPK01124.1"
                     /translation="MIVGLAGGVFAGLYWNVLYLLLEGSQRISGWGLLLLMPAAGLAI
                     GWIITRLGDPGEISLIVDNIHFRGGRLDVRNNPSMILSSLLSIASGGSAGPEAPMVQV
                     TGSFGTWFADRLGLRGEALRTLSIAGMAAGFTALFGAPLGGAIFALEILHHQHVVEYF
                     EALLPAVIASCSGYVVFAAVTHLGFGPTWVFPLLTANEIADFGLILLFGFLGAACGWL
                     FVTVFRGIGRTFERIPGPFFLKTGFAGLLLGTIAFVMPLTRFFGHDEVARVVTEPLTV
                     PALGALILLKMIAISVTIAGGWRGGIIIPLFFIGACLGKLAILVLPGQINPALAMVGV
                     MAGINASVTRTPISTAILLAKLTGTGALTPILFASLAGFLLAPRTPLIRAQLKRPPSG
                     SPLP"
     gene            18066..19217
                     /locus_tag="PLS90_01585"
     CDS             18066..19217
                     /locus_tag="PLS90_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK01125.1"
                     /translation="MTAQHVLVTGGAGFIGSHIVDALLERGHRVRVLDSLDPQVHGPG
                     ADGPPAYLNPAVEFVRGDVRDADALDRALDGIEVVFHQAAAVGVAQSMYAIRAYTDTN
                     VMGTATLLELIVGRHRDHVRKMIVASSMSIYGEGKYLDPETNEPVRIVLRDIPQMEAG
                     QWEPVVPGTHRPATPVPCDELKPLMPTSVYAVNKRDQEEMVLTTGLAYGIPAVALRYF
                     NVYGPRQALSNPYTGVAAIFSSALMNGRGPLIFEDGRQSRDFIHVRDIARANVLAMDS
                     DAADYQAINIGTGRPTSILQVARLLLERLHPDRRDDPQLQPRIVGKYRAGDIRHCYAD
                     IARARALLGFEPSITYEQGLDELIEWVRTQRPEDKSEQAYRELADRKLV"
     gene            19304..20380
                     /gene="purM"
                     /locus_tag="PLS90_01590"
     CDS             19304..20380
                     /gene="purM"
                     /locus_tag="PLS90_01590"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPK01126.1"
                     /translation="MAPAASARRTPRPLTYAQAGVDIAAGDELVDFLKAVNPAIGGFS
                     GLFSLPSGLRRPRLVASTDGVGTKLLIAQLAGRHETVGIDLVAMVVNDLVVCGARPLF
                     FLDYFATGRLKQHEARAVLKGVIDGCAQANCPLLGGETAEMPGMYAPGHYDLAGFAVG
                     VVEADRVIDGRAVRSGDVLLGLGSSGLHSNGFSLVRRVLLPGGEPAQTRRALRRRPWR
                     GGPTLADALLEPTRIYVKPMLDLVGRFPIKAAAHITGGGIAGNLVRVLPPGVRAWIDL
                     DAWRPQPIFDLIAREGPVEETEMFRAFNMGIGMILVVAPDVAGRVAARAERLGETCRP
                     IGWIDSAPRRKAPPIVELVRRSQQ"
     gene            complement(20522..22357)
                     /locus_tag="PLS90_01595"
     CDS             complement(20522..22357)
                     /locus_tag="PLS90_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK01127.1"
                     /translation="MSKRRSARSQQKSAKGKPAAPAAATRAAAAPYEPRPWRPPWTAV
                     LGLIAAALALRLVTVWLLYRGNPLFAYTMPGYDQNVYQDWAKEILAGDWLVRKGGLFY
                     YAPIYAYFAGIVYKICGGQNFWALHLIQALIGALTCGLLFRLAGTWLGRVGAWTAGIV
                     WAGLAPWLFYEQGLLHEGLLIMLYAVTLWSARRIELGARAPWALAFLAGSCIFLGTLA
                     RGNALALAPVLAVWLLFARPEGLRRPESWARAGWPAAVALCAGVALMMFLFLLRNRAV
                     TGAWEMGMKNGTVLFYLGNAADAVGYFTETPRFQEVHALAGADMSIYWREWFVDLRNA
                     PGHILLNLFRKAYFFFASPDLADNLSYPLARRLYPTIGLNPLQWVWLVPLGFVGAAVS
                     LRWWRRWSLLLIFAGVFSATLIMILPTGRYRIPLFIPVAIWVGLTAEWCLHQWRTRRY
                     GPLLAAGGAFAALVILLGPWWRPEAAKPRLNDYVNLTGAALDQGHADQARRLLREFER
                     DYPALAHRLPQLSRARFRLARLEGNQAEAVRLADQLWGAAQQAGPGFLTPQVAQEMAE
                     VYIAAGRRERASLIARSLYAGEETRALGQSLLEELGESPGTAAAP"
     gene            22499..23446
                     /locus_tag="PLS90_01600"
     CDS             22499..23446
                     /locus_tag="PLS90_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01128.1"
                     /translation="MLLELDNVTVRYGARKAIDQVSLRLESRALGLLGPNGAGKSTLI
                     RALLGLQPLAGGTARLEGLDIRRQGKRIREIVGYMPEHESYLGSMTVVRFLRFMGELC
                     GLPPGAAMERAHEVLFYVGLGEVRYRKMEGFSYGMQQKVRLAQALIHGPRVLILDEPT
                     NGLDPAARDDMLRLIQDMVRNSGSRLIISSHLLKDIESCCEEVVVLNKGRVTAAGNIA
                     AMKQTDERRFELRIKGPLDPFVAALSELGCQCRLGKREALQVVLPEHLPPRRIFEVAR
                     GQRVQIRHYYARKDTLEDVFLKALEDDAGEREVMADAGL"
     gene            23433..24314
                     /locus_tag="PLS90_01605"
     CDS             23433..24314
                     /locus_tag="PLS90_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK01129.1"
                     /translation="MPVYDLGYRVWEGKRRGPLLRWLAIPRFAYMDLAGRKAAIYVFS
                     MAWMPFLLLAGYIYLLVNYRFLQTLGIPARILFPIDAALFNLLLDLQLFFCFIFAVML
                     SPDLIARDLHHRAIVLYFVKPISRWEYLLGKFAILMSLCLLLTWVQPALLFLIQWAVR
                     PEDPTWRTTLWRADLGVLRAITLYGWTASAGVTLLALALSALVRNPRHVATTLLVFPV
                     GAWMVAGVLAEVLGTAFRVLSPLHAMRELGTWFFGLPTDARAPVWAAWLSWGGLAALC
                     ALILVWRLRRAARGGVA"
     gene            24311..25252
                     /locus_tag="PLS90_01610"
     CDS             24311..25252
                     /locus_tag="PLS90_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01130.1"
                     /translation="MTHGPVTIELRDVSRWYGEVLGVNRVTADLRAGLTGLVGPNGSG
                     KSTLMNLICGLLRPGRGEIRILGQPVWNNPELRRVLGYCTQNDHFYESFSGREFVASL
                     LRLHGHGLDWARRTAADWLERVGLGPHMDRRLRTYSKGMRQRAKIALALAHQPRVLVL
                     DEPFNGLDPVGRREMMQLLQEFAAEQRTIVLSSHILHEIEQMTDNILMMSNGYVVAEG
                     EVGQVRDLLTDHPFQVFIRCDDPRRLGALLLREPGVLSVQVEGERSLTLATRAPDAFY
                     LRLNELILDHGIDVDIVTLADENVQSIYRYLAGREHH"
     gene            25261..26106
                     /locus_tag="PLS90_01615"
     CDS             25261..26106
                     /locus_tag="PLS90_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01131.1"
                     /translation="MATPTPNNRTASALLAFWGAVRLSLARLGRTTSMLAVIFMALLP
                     LLIVVLIAAATWHEPIFHQPAAAQRVYQTILRLVGLHFVAFFIAYILGQAVVRQDLED
                     ETLHYLFLQPMPRWLLFAARFVAFFLLAGAAVVLGMWLCWLAATVPWMRPADLFDLLR
                     EQGRLAGLLREAGVLLLAVLFYGAAMIAVGSFFRSPFFAGLLLGWEAGVYYLPRALKY
                     WTGSHYFHSLLPDGGAERRLFELIGEPAGVGLCLTLIPAASMVLLAIGAAAFHLRECQ
                     YGKTN"
     gene            complement(26131..27276)
                     /locus_tag="PLS90_01620"
     CDS             complement(26131..27276)
                     /locus_tag="PLS90_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01132.1"
                     /translation="MADSSGSSVRPRRIPFALRAGIVLLAILLTFLFIWLLGFVLDDI
                     GDLNPPDYNALMDAATPADLTERQAALGKELQRIDREIARRRERQATLQEGMDNARSL
                     MDQMIKLHQLYLERGEGAPPDQEALLLSQERFLEAQNQFEQINNEIAGLNEAQHAAQD
                     EQNAIAERIEEILQPAREQYEAELTRHRLLVGSLKLAFIVPVLLAAAWLTVRRRASIY
                     RPIYIALCVAAFWKVAGVMWDYFPREFFKYIAIAAALVVTIGLLVRLLRAVARPRPLD
                     LLKRYREGYKSQHCPVCDYPIGRGPLRFAVWTRGGPRALAAGGETPPLEDTPYTCPAC
                     GTPLYERCGACGWLRHSLLPHCESCGARNDGAESVNGTQRVEGTEAT"
     gene            27445..27795
                     /locus_tag="PLS90_01625"
     CDS             27445..27795
                     /locus_tag="PLS90_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01133.1"
                     /translation="MNPPRAAVLPGWHCAACGGQVRKDDRVCSHCGARLVQPAPWHEQ
                     PVWIAVLILTLLGALGIPLIWRSRQLSRPAKWLFTLATILYTLIIILLLVWISILLWE
                     HLQAFQMQMDDLGM"
     gene            27829..28467
                     /locus_tag="PLS90_01630"
     CDS             27829..28467
                     /locus_tag="PLS90_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPK01134.1"
                     /translation="MRLVSLNVARPAEIPWQGRMIRTGIFKAPVEGPRRVSRLRIEGD
                     LHGDLSVHGGLDKAVYGYPREHYAFWRAELGAADDFPFGQFGENLTTEGLLERDACLG
                     DVYRIGGALLQISEPRQPCYKLAYRMGRPGFDARFLASGKLGYYFRVLEEGVLQAGDE
                     IVRVEPDPQRVSMLELASLLYLGGGDKDLLERALAIDSLSPKLRSRLARLER"
     gene            28800..30818
                     /locus_tag="PLS90_01635"
     CDS             28800..30818
                     /locus_tag="PLS90_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HPK01135.1"
                     /translation="MKYLPTSRIPALLLLVGVLTALPVLGPAEPMIEDADNPENNLGY
                     VTPPDLTREVPMIEVINDPVEGYNRAIFSFNNWFAMWVMRPVTKGYRAIAPLQVRLSI
                     RKFGHNLAYPLRLVTTLLQGKFKGAGVETARFLVNTTVGLLGFFDPAGSKLGLRPYDE
                     DFGQTFGKWGSGPGFYFVLPFLGPSSGRDTLAWPFDAVLNPASWAPGLGQFLMVNTLS
                     FSLPTYLRLMQTEMDPYILTRDFAALARKREVLDYRPIETEIEPSPTLQAIFLDVRDP
                     RFPDAGATAQVKIPSTGRELPYSYWLQPTPAPLIFIMGGVGNHRLSESAMAFAEIAYT
                     HGLSAVTLSSAMNWEFMEHAATVSVPGYTPVDAMDVYRAIDAIHQDLQQRHGANITAI
                     GVMGLSLGALHSLYLSAFENERRGGLVPIDRFVAVNPPIDLFYSMRKLDEYYNAPLQW
                     PEAERAARIQNTLFKAVQLTEMDLTPKTDLPFDAIESQYLIGLNFRLILRDIIYTSQR
                     RNNMGVLATPLDTVARQAVYDEISQFSYLEYVDRFVVPYYLSHNELAATADELVSRSN
                     LRWIEDSLAGDEDVRVYLNEDDFIIDREDIPWLRSVLGGRLTVFEGGGHLGNLYRPEV
                     QAELVSSLVELRDNPPRRDAAEDAAPAVDREPVLLPAVSLPAPADAAL"
     gene            complement(31050..31448)
                     /locus_tag="PLS90_01640"
     CDS             complement(31050..31448)
                     /locus_tag="PLS90_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01136.1"
                     /translation="MASQPHARSHRRCIRFGRSAIAGFTLIEIMIVVAILAVILAIAG
                     PTWIRQRGLSQQRVCQENLQKIEGAKEVWALDFKQPPDAVPGWDDLVQPDGSGYLKSE
                     PSCPSSGVYTIGAVSDDTTCSVNEPFDHNE"
     gene            complement(31656..32021)
                     /locus_tag="PLS90_01645"
     CDS             complement(31656..32021)
                     /locus_tag="PLS90_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01137.1"
                     /translation="MKFFRKTSKGFTLVEIMIVVAIIGILIAIAVPGFVRARTQSRNR
                     ACQENLTKIDGAKEQHALENDLAPGAAVAMSDLVTGDPTTSYLKVEPECPESGTYTVG
                     AVGTDPTCSVGGDHALDAI"
     gene            complement(32316..32786)
                     /locus_tag="PLS90_01650"
     CDS             complement(32316..32786)
                     /locus_tag="PLS90_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HPK01138.1"
                     /translation="MRFHTTRFGEIEFPEEVVMTFPEGILGFPDEQRYILLEHDTDGS
                     PFKWLQSVENPALAFIIVDPLFIDAGYHLEIDLDTARMIGTDAVEGCAIMSIVNVPHD
                     APIRMTANLKAPLIVNVENRHGRQIVLGNNAYSLSTPIFQHLVEAEPLPAAAVS"
     gene            complement(32789..33073)
                     /gene="csrA"
                     /locus_tag="PLS90_01655"
     CDS             complement(32789..33073)
                     /gene="csrA"
                     /locus_tag="PLS90_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HPK01139.1"
                     /translation="MLILTRKSGEGIRIGDSITLKILEIRGNQVRVGVDAPRNISVHR
                     EEIYDLIREQNALAALSSPDSSEVLTSIWRDQQSLTQRPRNLSHNGNGKA"
     gene            complement(33446..34216)
                     /locus_tag="PLS90_01660"
     CDS             complement(33446..34216)
                     /locus_tag="PLS90_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPK01140.1"
                     /translation="MTETGTQGAPSPPRLLEFDLSTPDGNLRGMLAIPPGPMRLAELA
                     RSTLPLVDRLVELATRREQRAGRVISCGPGCGACCRQLAPVSPPEAYMLADLLMHLPA
                     ERQARYLERFEAAASRLEAEGLSEALLRAPESDEAARALAARYFLLGIPCPFLEEESC
                     SVHGGRPSVCREYLVTSPAADCADPAANPIRRVGVSVRLSEALARLHAELFGGEPQLV
                     PLSLAISFALEHIPEGRHAWDGVALTRRLLALMSGAQA"
     gene            complement(34244..35707)
                     /locus_tag="PLS90_01665"
     CDS             complement(34244..35707)
                     /locus_tag="PLS90_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01141.1"
                     /translation="MSLKVELTVEKTDLAFDENTRFEVTITNVGRESVMTMNPADCWT
                     LPALRVVSIEQGLDRIYRRDPNLFIEEVPSPLLPGQSLSGTHRLLTFVKFQEPGEYEI
                     SALWEWNGKVGRAFSEPVHVTIRAMRPRHPHTVSMSGGPAAIFNSVWVNVATPPGSLT
                     RSRYDLINKPKTVHLQSLGQIGPSAEPVLSVPINGGASDRQWIAWFEGDELRFGRVDD
                     LTEQLDIKTLPLSPVPKQIVPPLNHQPGVAGLEGRFFLWQGNVEGDISRFQAVHLTTR
                     GDAEPGANVMLGRLRPRWMEAAFPSNGSAQIYFVLPTQGENFLLQAVDWSADGEPSEV
                     KKLAQFDGEFHGGATALAADDTLHGTLLVLEPGPEAARELGLYCWKRSAAGQFEASDI
                     LLVPREPMQKVDRAVVGVSSKGDPALLIRFQGSDWQFYDEQHGLVPLPAEIAQNDLPL
                     ELLFLISGTPVIKYAVENKGFYYLSAAGIPVETPAHG"
     gene            complement(35727..>36743)
                     /locus_tag="PLS90_01670"
     CDS             complement(35727..>36743)
                     /locus_tag="PLS90_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01142.1"
                     /translation="DDQAVAITTGTMVVWRTIRLSRYIFRKDPAMANFPAAIEAAYAG
                     PPYNLNKETIYLRCGLIKQDLTYVGMKQADMTPTGLRDNGPVGAGNWEGPARQYAKAW
                     CEFIIDPNVAIPEEPDAATQRAAFDAGVAYAKNFQGAMGVNWDLDKLFFFDARTPFTI
                     NIREPAEYDALPGVTPAQKIGPIPPAAGNDPRDTMSMVLNEFLNGMLSYYSKKGSLPG
                     LTFIQYPMGCIWDVTSGALRGPTNGPHMTSGVSLHFRGAYLWYGERIYSNQPSFPYNV
                     SSNTCHELGHSLYAVHQPSPFGGGIAARHDAADWCVMSYNFCEGQYCGKTLALLQGLD
                     IAKF"
BASE COUNT         5855 a        12647 c        11956 g         6285 t
ORIGIN      
        1 cccctcggcc gtccctcaac cggcgcccat tttccgcctg attcgccgct cgccattctt
       61 tgctttgcat ggcatccacc cttccggtag aatgctgcgt tttgtgtcca acgggacgaa
      121 accagactgg gcgggccggg gccctggcgc ccccgccgat accgaagggt gatgatggat
      181 ggcacgcaag ttcccactcg ataagatccg caacatcggc atcatggcgc atatcgatgc
      241 cggcaagacg accacgaccg aacgcatcct gttctacacc ggccgctcgc acaaactggg
      301 cgaggtgcac gaggggaccg ctacgatgga ctggatggcg caggaacagg agcggggcat
      361 cacgatcacc tcggccgcca cccagtgcta ctggcaggat cactgcatca acattatcga
      421 cacgccgggg cacgtcgatt tcaccgtcga ggtcgaacgc tcgattcgcg tcctcgacgg
      481 cgcgatcgcg ttgttctgcg ccgtcggcgg cgtcgaaccc cagagcgaaa cggtctggcg
      541 ccaggccgac cgctacaaga ccccccggct ggcgttcgtc aacaagatgg accgcgtcgg
      601 cgctgatttc ttcaactgcg ttcagatgat gcgcgatcgg ctcaacgccc accccgtccc
      661 gatccagatc cccatcggcg ccgaggatca gttcgagggg atcatcgacc tggtggagat
      721 ggtcgcgcgc gtctggccgc tgggcgccga cgaggaacgc ggccgcgtct tccaggacgt
      781 gccgattccg gagaacctgc gcgatctggc caacgagtgg cgcgagaaga tgctcgaagc
      841 gctcgccgaa tacgacgacg acttcgccga ggcctacctg gaggagaagg ccttcgagcc
      901 gaaggaactg cacgaccacc tgcgcaaggc gacgatcgcc tgccacatca ccccggtgct
      961 gtgcggcacg gcgttcaaga acaagggcat ccagttgctg ctcaacgccg tcctgcgcta
     1021 cctgccctcg cccaaggatg tgccggcggt gcatgggcaa ctgccgcacg agcccgacac
     1081 ggtggtgagc cgcgagccga gcgaagacgc gccgttcgcg gcgctggcgt tcaagatcat
     1141 gagcgacccg catgtgggcc ggctcaccta cctgcgcgtc tattcgggca aggtcaaggc
     1201 gggcagctac gtctacaacg tcacgcgcga ccagaacgag cggttgggac ggctgatgct
     1261 gatgcacgcc aacgaccggg aacagatcga cgaggtccgc gccggcgaca tcgtcgccgt
     1321 catcgggttc aagaacacga cgacgggcga cacgctgtgc gatccggaac acccgatcat
     1381 cctggaggcg atgagtttcc ccgagccggt gatctcgatc gcgatcgaac cgaaaacccg
     1441 caacgaccag gacaagatgg gctcggcgct gcagaagctc gccgaggagg acccctcgtt
     1501 ccacgtcaag gtggacaagg agaccaacca gacgatcatc tcgggcatgg gcgagctcca
     1561 tctggacatc ctggtcgacc ggatgaagcg ggaattcaag gtcgaggcca acgtcggcgc
     1621 cccgcaggtg gcctatcgcg aaacgatcac caagacggtc gaggtggagg gccgtttcgt
     1681 gcgccagtcg ggcgggcgcg gccagttcgg ccactgctgg ctgcggctgg agccggtcga
     1741 gccgggcagc ggcttcgaat tcgtcgatga agtggttggc ggcgtcatcc cccgcgagta
     1801 tatcccggcg gtggaaaagg gcgtcatcga ggcgatgaac aacggcccga tcgccggctt
     1861 ccccgtggtc gacgtcaagg tgaccgttta cgacgggtcg ttccacccgg tcgacagctc
     1921 ggagatggcc ttcctgatcg ccggttcgat ggcgttcaag gaaggcgcga aaaaggccgc
     1981 gccgaacctg ctcgaaccga tcatgaagct ggaagtgacg acgccggagg attatctggg
     2041 cgccgtcgtc ggcaacctgc aacagcgccg ggcgcatatc gacgagatgc gcgcccgcgg
     2101 ctcgatccag gtgatctcgg cctccgcgcc gctcggcgag atgttcggct acgccaccga
     2161 gctgcgctcg cagacccagg gccgcgccag ctactcgatg cagttctcgc actacgcgcc
     2221 ggcgcccaaa tcgatcgtcg aaaaaatcac gagctgattg cggcaggcgg tttacccctg
     2281 cgccaggcgg gcaagcagct tcaccagaaa gtcataagtc cgcccgacgg agccgaggct
     2341 gacccgctcg cgcggcgagt gcgggtcgcg gatcgtcggc cccagcgaga tcatctccat
     2401 ccccgcgatg cggtcgccga tcaccccgca ttccagcccg gcgtggatca cgttgatctc
     2461 gggttccgcc ccgctcagct cgcgccacac ctcgcggcag aggccgttga gccacgcatc
     2521 gcgccggggc ggccagggcg gatacccgcg gccgtcatcg gcggcctcgg cgccggcact
     2581 ccgcgccagc cggtggatgc gttcggtcag cgactgcatg tcctcgcgcg acgagctgcg
     2641 ctggctggtg atgatccgca gcgcgtcggg ccgcgtctcg acgcgcgcca ggttgcagga
     2701 ggtttccacc acctcggcga actcgcgcga catcgtttgc ggcccgtgcg gcaactcgcc
     2761 cagcagcacg cgcagccgtt ccgcgcagtc ggccgtcagc ggatgccccg tcacctcctg
     2821 agagacgggc tccaggctga gtgtggtctc cggatcgcgg tcgccgtact cgcgccgcat
     2881 ccgctcctgc tgcgtggcga tcacgtgccg ggcatcgttc agccggtcgg ccacgagccg
     2941 cacgacggcg cgggcatcgc gcgggatggc gttgtcggcc gagccgccat cgacggagac
     3001 cagccgcgcc ggcgccgccc cggccagcgc gctccacgct tcggccagca gcttgagcgc
     3061 attggcgcgc cccagatgga tgtccacgcc cgagtgaccg ccgcgcaggc cgcccacgcg
     3121 cagcgcgtag agctcgcccg gccccggctc ggcctcgcgc gccaacggca gccggatcga
     3181 tgtgctccgc ccccccgcgc aaccgatcgt gaagaccccc tcctcctcgc tgtccaggtt
     3241 gagcaggatg cgcccgctga cgattgacgg ctccagcgcc cccgcgccga tcagtccggt
     3301 ctcctcctcc accgtcagca ggagctcgag cggtggatgg atggcggcgg ggtcctcggc
     3361 cagcgccatc atcatcgcga cggcgatgcc gttgtcggcg ccgagcgtcg tgtcggtcgc
     3421 gctgagccat tcctcgccgt cctcatcgcg ctcctggcgc aggctgatcg gatcggtcag
     3481 gaaattttgc gccgattcgg gcgttttctc gcagaccatg tcgacgtgcc cttgcagcac
     3541 gacggtggcc cgggccgcca tccccggcga ggccggaacg gcgatgaccc ggttgcccct
     3601 ggcgtcgcgg cggaccgcga agccgtgccc accggcccaa tcctccagca tccgcatcac
     3661 cgcttcctcg cggcccgaag ggcgggggat gcggctgatc gcctggaagt gcttgaggac
     3721 gcggtcggat gaagcgggca tcggcgtatt cccatctgga tcggggcgga gctggctggc
     3781 ggttcccgag ggtgttgctg ccggcgtccg ggccctagcc ggcgctgaag ccgctctcgc
     3841 ccgcgccgaa gctctccggt agcaggtcgc gcaaggcatg gcgccgcggc gcggttccgt
     3901 cgcagaacag gtagaccgtc atcgtggggc cgaactcggc cagcacctgg cggcaggcgc
     3961 cgcagggcga cgtcggcacc gccgtcggcg tgtagaccgc cgccgcgagc agcttgcgcg
     4021 cgcccgccag caccgccgcg ccgacggcat ggcgctcggc gcagatcgtc aagccgaacg
     4081 aaacattttc cacgttgcag ccggcgaaga gccgcccctg gtcgtcgagc accgccgccc
     4141 ccaccgcaaa gtgcgagtac ggcgcgtagg ccgccacggc cgcctcacgc gccgccgcaa
     4201 acagacggtc cagatcggcc ggcgagacgg gctcgggctg cggcacggtc gggcgctcct
     4261 caggctggat tgggcgcggc gggttgccaa tccgcttcat tcaatccgaa gcactcgccc
     4321 gcgccaatcg aaaatcgtgg tccgcaggct ttccgcagct ccccccgctc acaaggcttc
     4381 gatgatccgc cgggtcatct cctgggtgcc gatcgatttc tcgttgcggt cggcgatgtc
     4441 gcgggtgcgg tggccggcct cgagcgcccg ccggacggcc tgctcgatcg cggcggccgc
     4501 ctcctccatc tcaaacgaat agcgcagcat cagcgcgccg gagaggatcg cgccgatcgg
     4561 gttggcgatc ccctgcccgg cgatatcggg cgccgaaccg ctgatcggct catagaagaa
     4621 cggcggccgc ggcccgatcg ccgccgacgg gatcatcccc agcgatccgc ccaggaccga
     4681 cgcctcgtcg ctgatgatgt cgccgaattc gttgttcgcc aggatcacgt ccagctcggt
     4741 cggccgctga accaccacca tcgtcgccga atcgatgagc tggtggcgca attcgatgtc
     4801 ggggtagtcc gcgccgacgc ggatgaccac ctcgcgccac agccgcgagg tctccaggac
     4861 gttggacttg tcgagcgaca tcagccgctt gcggcgccgc ccggccgcgt cgaacgccac
     4921 ccgcgccacc tgctcgaccg ccttttccga atagacgcac gtatcgtagg cttcgatgtc
     4981 cccttcgggt ccgggccggc ggccgcgctc gccgaaatac agcccgaaat tcagctcgcg
     5041 aacgatcagc atgtcgacgc ccccctccag cagatcgggg cgcagcggcg tccggtggcg
     5101 caggcattcg ggcagccaga tcggccgcag gttggcaaac agtccgaaag tcttgcggat
     5161 ggagagcacg gccttctccg ggcgcagggc gagcggttga tcgtcccact tgggaccccc
     5221 ggccgccccc agcaggatgg cgtcgctccg gcggcaggtc tccagcacct cgtcggtcag
     5281 cggcagcccg tgcgcgtcga tcgacgcgcc gccgaccagg cactcgcgcc actcgaaccg
     5341 cacgccgcgc ccgcgggtgg ccgcctccag caccttgagc gtctcgggga tgacctcctg
     5401 gccgatcccg tcgccggcca gaacggccac cttgagcaca cgatccgtca tctgggattc
     5461 cctcgcttca ctgtcgattc atgcggcggc aggcacattt cccgcgcgct ccgcttgccc
     5521 tcaggtcaat tccaccggga tctgcagcag ttcgcggtag agccggatga tcatcaaatt
     5581 ataatgcgcg tcatcgtcat tggggttgat gctcagcacg cgctcgaacc cgcgccaggc
     5641 ctcggccatc ctccccgcct caaagtattt ccaggccagg ttgtaaagga tgtcggcgtt
     5701 gtcgccgttg agtgccgcca gcgcctcata ttccccgatc gaatagtcgt agggcgaccg
     5761 gttgcgctcc atgccgggcg gccagtggat gttgcgcgcg atcttgcgtg tcgtcgcccg
     5821 ccgggtcagg aactcgtagg ccttgcgcag ccgcatgaat tcctcgccgg ccagttcctg
     5881 cagccgcgga cgcaggtggc gcaccttgtc ggggtgaaac agcttggcct tgcggcggaa
     5941 ggcgcgcgtg atctcttcgt ggccggcgcc cggttcgatc tccagcaccg cgcagcaccc
     6001 ctccgccgtg gtgaggtcgg gctccaccgc gcgcagcggg atctgatgcg tatgctcgat
     6061 ccggtcctct tcttcttccg tcggggccgc cggctgctct ccgcgcagga tctggtccgg
     6121 atcggcgatg acgcgcggcc gcttctcacc aatcagccgg cagcgcatct cgcgatactt
     6181 gcggtcgtcg atgatcgccg attggaaatt gagcatctgc gccggcgcct catcgtccag
     6241 caccgcgccg gtcagatcgg catcggtcac gtcggccccg tcgacgacgg cgccggtcag
     6301 gcgggcgaat ttcaacaccg cgccccgcag gtcggcctcg gtcaggttcg agccggagag
     6361 gtccgcgccg accaggttac agccgctgag ccgcgcctgc gacagattgg cgccctccag
     6421 gcacgtatcg accatctcgg cctcctgcag gttggccgag cgcaggtcgg cgcggtcgaa
     6481 gcaggaattg cgcaggttgg cccgccggaa atcgctgaaa ctcagaatgc tgtccgacag
     6541 gtcgcagttg atcagctcgg cgccgcgaaa aatcgccgtc gacatgttca gttcgctcag
     6601 gtcgtaatcg cgcagtttca aggcggagag atcggcggtc gacaggtcga tccatttggc
     6661 ccgcagtttg aacaacagcc agtcctttcg gccggtggac ttgtcgacgg aatcgcgcaa
     6721 gcgcttgagg tgcaggggat cagccatgcg tggtcggata cgcgaagaaa tccctcagcc
     6781 ggacatcggt tcatgccgac aacttgcacg cggcggcgcc ctgctggcac aatgaggcca
     6841 gtctgcgccg gcccgtcgcc gcgcgctggc tttcagcgcc ggcggccatg gtcgctggca
     6901 ttctatgcat cgagcgaacg atgagccaat cttttgtcga tagcgtccgc gtttatgtca
     6961 aggggggcga cggcggcaac ggcgcgatct ccttccgccg cgagaaatac gtcccccacg
     7021 gcgggcccga cggcggcgac ggcggccccg gcggcaatgt ctggttcgtc gcttccactg
     7081 aacttgccac cttgattgat ctgaagttac gccccagcct gaaggccggc gcgggcggcc
     7141 acggcctcgg caagaatatg accgggcgcg ccggcgacga catcaccgtg gaggtccccc
     7201 tggggaccgt cgtcctcgat gaggacggca acgaactggc cgacctggtg gaggccggcc
     7261 agcgcttcct ggcggcggcc ggcggcgccg gcggcgccgg caaccagcat tacgccacgc
     7321 cgaccaacca ggccccgcgc aaggccaaac ggggccagcc cggccaggag cgcagcctcc
     7381 aactggaact gaaactgatc gcccagggcg gcctgatcgg cctccctaac gccggcaagt
     7441 ccaccctgct ggccagcctg acgagcgcca ccccgcgcat cgcccccttc cccttcacca
     7501 cgctccatcc gaacctgggc gtgatggagg tcgacgtggc gcgccgcgtg acgctggccg
     7561 acatccccgg cctgatcgaa ggggcctggc gcggcgccgg cctgggcgac cgcttcctgc
     7621 gacacatcga gcggaccgcc ctgctgattc acctggtggc gcccccggag gactgccagc
     7681 tcctgcccga cctgcggccc gaggacgagg cggagatggc cgaactgggc cgcgacggcg
     7741 tgcgcgcggc ctaccagctc gtgcgccgcg aactggaggc ctacagcgag gaactgagcc
     7801 gcaagccgga aatcatcgtt ctgaccaaga tcgacctgct gcccgaacgc ctccgccagg
     7861 cctggctggc cgtcctgcgg gagaccggca agccggtggc ggcgatctcg gccgagaccg
     7921 gcgagggcct ggatcgcctc aaagccctcc tgattgagca actcgatcgc atgggcatga
     7981 tctgggacga tccgcgccgg ccgcatctcg ccgcagcccc gcccgtcgaa cccgatcgaa
     8041 gcggggattg acgacacccc gccgaccggt taagacaggg gacggcgcgc gaccgtcgcc
     8101 cgccccacag gcccgatcga tggccgcccg ggtgagaccg acgcgcgatg tccagatgat
     8161 ccacatcgac aacatacaag ggaccggccg cgggatcgac ctgcgcaagg tggttatcaa
     8221 gctcggttcg gccgtcgtcg ccggcgaagg ccaccagctc gacttggtca cgctgggcgc
     8281 cctggccgat tcggtcgcca atctgcgccg ccagggcgtg gacgtcatcc tcgtctcctc
     8341 cggcgcgatc ggcatgggtc ggcgcgcctt ccccgatttt accgcccgca cgatcccgga
     8401 ccggcaggcc ctggcggcga tcggccaggt cggcctgatg cacgcctaca aaaccctgtt
     8461 caacgcgcgc gatctgcgcg ccgcgcagat cctgctcacc cgcgacgaca tggaggatcg
     8521 ccgccgctat ctgaacgccc gctacacgct ggagcgcctg ctcgaactgg gcgccgtccc
     8581 ggtgatcaat gagaatgaca ccgtcacgat cgacgaattg cagttcggcg ataatgacga
     8641 gctgtcggcc gtcgtcgcga tcaagatggg cgccgacatg ctcatcatcc tctcgatcgt
     8701 cgatgggctg cacgaggccg aagcggatcc caccgacgcc ggcgcgcgcg gccgcaagcg
     8761 caagggccgc cacgccgccg gccggcgcat cgacgtcgtc gaatcagtgg acgacgtgct
     8821 ggggctggcc gaccggacgc ggtcggcgat ggggaccggc ggcatgcaga gcaagctcaa
     8881 ggcgctgcac atggcgagtc aggcgggcgt gcatgccgtg atcgcccccg gcaagcgggc
     8941 cggcatcgtc gaggagatcg tttcggggcg atttcccggc acttacttcg tcccgcgggt
     9001 cgggcaccgg ttgcgcggca agccccgctg gctcgcgttc ggccgccgcg ccaccggacg
     9061 ctgcatcgtc atcgacgccg gcgccgtcga ggccctgcgc gtcggcaaga agagcctgct
     9121 ggcggctggc gtgcgcgccg tccgcggtca gttcgagcgt ggcgatctgg tcgaggtgca
     9181 cgacgaagcg ggcgcgcgcg tggccaaggg cctgaccaac tattccgccg aggaactgga
     9241 ccggatcaaa ggcaaacgca ccccgcagat ccgcgagatc ctcggcgaac tggactaccc
     9301 cgaagtgatc caccgcgaca atctggcggt cgtggattag cggcgccctc gaccggataa
     9361 aaaaatcccg gcgggccttg ccgccgggat ggtaatgagt ccgcctggat tgcaacgggc
     9421 gctcagcgct ttcgttcgat ctccaccgaa cggtcgccct tctccacctt ccaggcgatc
     9481 agggccgcgt cgccattgat ccggcccgga tataccgaca ggccgatcag gtcgccctcg
     9541 gacaactcca ggtcgcgcaa ctggtcggcg ggaccgaggt ccaccgtctc cattccgcgt
     9601 cccttcagat cgacccgggc gatgagatgg tcgccatcct gaccctgata gcggacggtg
     9661 cgcaggccct cgatgctccc atcgacctgg tgcagcgagg tgcgcatctc gcgttcgatc
     9721 ttgatcactt caccgttcgc cttcaccgtc tgggcgatca tcaccggttg gccgttcagc
     9781 cgtcccggac ggccttcgac gtagatcgaa tcgccctctt tcaggttcag atgggcgacc
     9841 ttgctggccg gcccgagttc aacccgcccc tggctgctct tggcggtatc cacttcggcc
     9901 agaatatgct cttccgacac gccaggatac tcgaccttca tgatccgtgt aatgtggccg
     9961 tcgatcggat agtagagctc gttggcgggg ctggggggcg acgcatcgta ggtgagccct
    10021 tcctcgcgcg cgcggatttc ctcgcgcgtg gtcagctctt cattctcttt gatgatccgc
    10081 tccttttcgg gttcgctcag atcggagagc gtggagctgg ccggcaggcc gaggacgacc
    10141 ggctcgcgcg accggatcgg ctcgcgctcc accgcgtacc cacggtcggt gtcgaccttc
    10201 gatgagccct caacctgatg ccactcaccc gacggtcccg tctcgaagac gatccgttcg
    10261 gtgcccacca gcggcttgct gccgttgagg tcttgcgccg gcggcatcgc cgtcgccggc
    10321 agaaccaggc ccattccgaa cagggccgcc aggaagggtc tggtgattgt ggtaagcatg
    10381 attcttacct cctttaacgt cggccagttt caaatggccg aattttcaaa aatgttaata
    10441 attatcggct cgttgcggct tgattccaga atgcgttctc ccgcacgttg acgcggtcac
    10501 gcgttcgata ttcccaatac caattcgaat cgcggtccca gcgatcatcg ttgtcgtacc
    10561 agtcgtaacg atcgtaatcg cgagtataga actcatagtg ttcgacccag ccgcggcgat
    10621 ggtccgaacg caagcggcgt tcccagtcgc gatccgaccg gtcgaagcgc gcgccatcgt
    10681 catcgcggtc tcggtcgcgg tcgaaatagc gttgcgcccg ggtcccctcg ccccccagct
    10741 ccggctcgtc atgccttgac ccgcgccggt ccggatcatc ggatcgaaga tgggtatcgc
    10801 ggtcgcggac gatgaacgag tcgcgatcaa gccggctcgg atcgcggtcg ggccgcgtcc
    10861 cgccgctaga gtcggatcgg gtccccgtgt cgcggcggat atcgatgccg ctcgggcctt
    10921 cctcggcgaa agcggccgga accgcgccca gcgccaggat cgcggccaag ccccatgcct
    10981 tccacatcgt tctcatcgtg ttgtgcctcc tgcaagaatc ggattcacgg tcaggatgac
    11041 gcattggagg gaaattccgg cgttgccttc gccgtccgtc cgcgctgcct gaagaaggga
    11101 gcaattcgcg ggccagccgg cgtcggtgcc atcgccgggc gccggcctcc ccgatcagtc
    11161 gacccaggct gttgtcgtct tgcaaaatcg cgccatcgcg tgtcctataa tcccaatacc
    11221 aattcgattg gcggtataac gggatgtcgt tgccattccg taatcgtcgt tcgtgtccgc
    11281 cttcccatgg gcaaaacccc cgggccaggg gagggcgcaa agctgaaacc cgaatccgtg
    11341 caacggcgcg gattctgccg ttcaacaaaa gcaattcaca ggccatttcg tcagcgccgc
    11401 gcgcggccgg caatgcttat agttgcgacc ggaaaagatt gagaaaatgc tccagcgcct
    11461 tctcgcgcag cggccgccgc cgccattccg ccagactcac ttcgcggctc agctcccggt
    11521 cgcgctcgaa gatctcgacc tgccgcgcgg cgaactcccg atcatgaatg ttgagattcg
    11581 cctcgtcatt cagccggaac gagcggttgt ccagattcgt cgaaccgacc gagacccagc
    11641 agtcatccac gatcatcacc ttgcagtgat acatcgtcgg ctggtattcc cagatgcgga
    11701 cgccggcttc gagcagcggc ccccagttcg cgcgcgacgc gcgccggacg gcctcgtagt
    11761 cgatcgttcc tccgggcaga atgatgtcca cggccacccc ccgctcgcgc gcccggacca
    11821 gttcctgctg ggcgtgctca tccggaacga aatagctgtt tgcaatccgc acgtggcgcc
    11881 gcgccgcggc cagcgagaac aggtacatca gccggacact gctgctcccc tcgttgggcg
    11941 aactcttgaa gacctggcag caggcgtcgc ccgccggttc ggccggcttg aaatacgcgt
    12001 cgccgtggag cacctggtcc gaggtcttga tccagttgtc catgaatgcc gcctggagct
    12061 gggcgaccgc cggcccctcc aattgatagt gcatgtcgcg ccagtggtcg ggatcctgcg
    12121 cgtcgccagt ccattcgtcg gcaatgccga ccccgccggt gaagccgatc cggccgtcga
    12181 tgacgagcag cttgcggtgc gtgcgatagt tcaggcggct caggttgaac cagcgcagtg
    12241 ggtgatactt gacgatctgg acgccggccc gctccatctg gtcgatgtag gtgctctcga
    12301 tcctggcgcc gaaccagtcg aggatcacat ggacgcggac gcccgcgtgg gcgcgttcgg
    12361 cgagtgcctc ggcgaaccgg cggccaattt ccccggacca gtaaatatag gtttcgaacg
    12421 tgatcgtttc gcgcgccccg gcgatcgccg ccagcatcgc gggaaagatc tggtcgccgt
    12481 tgcacagctc cgtcagccgg ttgcctgcca ccagcggcgg ccccagcaga ttgcccatcg
    12541 cgcggcggaa cggggcatcc tcgacgccgt agagcgactc gatccggtag gcgatgtatt
    12601 tctgccgcgt cacggcgccg gccccctgcc gggggctcaa gcaactgcga tgccgaactc
    12661 agccgcggcg cgccccgcgg gtgcgggcga cccgggcgaa atccatcgga tccgtcggat
    12721 atgactttgg atgctgagag gaaggtgagg ctcgccgccg cgccgatcag tcgtcgtatt
    12781 cgtcgccggc gccgtctttg gtgcagccgt cgcagatcgt cccctggacg cggccgcgca
    12841 ggaaattgcg ccgatagcgc gtgtagacct cgtcgtgcca gatctcgcgc agcggcttct
    12901 tggaggcgtc ccccatcacg ccggtctgct cccagtcggc gcagcacatg accatccggc
    12961 cgtcgtagag gatgtacatc tgctcgaaca tccggctgca ccagtcgtag ttctgcaact
    13021 ggcgcacggc gatctcgtcg ctcttgatcg ccttgtgctt gccgcggttc tcaagctgat
    13081 tgatgttgat cttgacgccg cggctgcccc agtactcctg gattttgtcc atctgcggct
    13141 cgatgtactt cgtcatcagc atgcagacgc ggatgcgcgg cttcttgtaa ttgcccgcgc
    13201 gtttgagctc cagcagccgg tcgatgttgt cgagcgtctt ctgcagcggc agcttcatca
    13261 cgtcttcata gatcaccgga tcgagcccct ggaccgaaaa attcacgcgg tccaggcccg
    13321 catccagaat ccccttggcc atccgctcgg tcagcaagcc cccgtgcgag ttgatcttgc
    13381 tgaactgcca cggcttcttt ttgcgggtaa tgtattcgac gcgcttggga aattccgggt
    13441 cgagcatcgg ctcgttcatc agatacggcg aaatccggcg gacccccatc tcgctgatct
    13501 gatcgatgat gctgcggtac agatcccagt ccatccggtt cgcgaacgga atctgcaggt
    13561 cggtcttctt gttggggcag aaaacgcaac tggcattgca gcccgagatc gtctggatct
    13621 ggacgttttt cgggacttcg gggaccggct ccagcttctc gatatatttg tgccggaacc
    13681 actggatcgg gcgggtcggt tccggcatgt agtacttggg cggcatgggc aaaatcctgc
    13741 gagatggact ggaccgggcg gggaggcttc agcgcgatcc gctgactgga gatgacccgc
    13801 ggcggagcca ggcggtttgg gccccggcgc atgatcgggc cccggccgga ttcaaccgcc
    13861 ccgtgggccc ccccttttta ccgtagaacc gttattgagt caactcgttg ggcagggaca
    13921 taaaggacat cagggacatc aaggatggaa acaggccata ttcatccttt cagcgtgatg
    13981 ccgaaggcgt cgtgaaaagc cttctccaaa aatagttcct cgtcctcgtg gttgctccga
    14041 aggtctaccc cgaaattcgg ctctcggctc tcgtcccgcg tcctcggctc tcgaaaaccc
    14101 gcggattcta ttcccgcgtt gtgaaccgca gcttcgtaag caactcgtaa tcttccgctg
    14161 tcgcactcat tccgggcccc ggcttgctct tgttgttgag tttgtgatct actggggtgc
    14221 gctccgaccc gcaccggcgg gcccgggcca tcccaccccg agcggctttt ttcatgttca
    14281 tcctggggat ccacgacggt cataacgcgg cggcctgcct gcttgaggac ggccggatcg
    14341 tcgcggccct gcaggaagaa cgcctgacgc ggatcaagaa tcacgacgtc ttcccggcgc
    14401 gcgcggtcca ctggctgctc gaatcgcgcg gactgacgtt gcgcgacatt cacgccgtcg
    14461 ccatgaacgg ccatcatcag ccggcccacc gcgaccgggc gcggctgatc caggacacgc
    14521 ggcgcggcgg cgacctgcat ccggcgcgtg cgttgcgccg gctcgcgcgc aaaacgccgc
    14581 tgctcgccct ctggcgccgg cgccggcgcg ccgaccgcga ggccgaagcg cacgccgccg
    14641 gcatcgaccc ccagcgcctg gcctggatcg agcaccatcg ctgccacgcc gaggccgcct
    14701 ggtggggctg cccctggcgc gatgggcccg tcctggtgct gacctgcgac ggcgcgggcg
    14761 acgacctgtg cgctaccgtc aacgtcgcca acgaggacgg ccggggcggt ctgcgccgca
    14821 tcgcccaggt gcatgagtcc cattcccccg cgctgatcta cctggtcgtc accacgctgc
    14881 tcggaatggt ccccaacgag cacgaataca agctgatggg catggcgccc tacgccgggg
    14941 aacaggcgac cgaggaggtg gcgcggctct tcgagcgctt gctggagtgg gaccccaacc
    15001 agccgctggc gtggcgccgc cggccgggcg tccccaatgc ctattacagc tacgattacc
    15061 tgaaagcgat gctcgacctg cgccggttcg atgcgatctg cggcgggatg cagcgctggg
    15121 tcgagcggct gctggccgaa tgggtgcgcc gctgcgtcgc ggcgaccggc ctgcgccgcg
    15181 tcgcgctctc gggcgggatc ttcatgaacg tcaaggcgaa ccaggtgatc gcccaggtgc
    15241 ccgaagtcga cgacctgttc atcttcccct cctgcggcga cgaaaccaac acgatgggcg
    15301 cggcctgggc cgcctacgcg gggatggcgg cgcccgacgc gccgcggccc gagccgctgg
    15361 ggccgatcta ctggggcgcc gcccctgcac cggccgagat cgaggcggcg ctcgccgcgc
    15421 agggcgcgca tttccgctgg agccggccgg cggaccttgc cgcgcacgcc gccgcgttcc
    15481 tcgagcgcgg cgaggtggtg gcgcgctacg ccggccgcgc cgaattcggc gcccgcgcgc
    15541 tcggcaaccg ttcgatcctc gccaaccccg cccgccccga ggtcgtccgg cgcatcaacg
    15601 cgatgatcaa gagccgcgat ttctggatgc cgttcgcctg ctcgctgctg gccgagcgcg
    15661 ccgatgatta cctcgtcaac cccaagcgcc tccgcgcccc ctacatgatc ctgacgttcg
    15721 acacgaccga ccgctgggcc gacatcgccg ccggcaccca tccgagcgac cggaccgtcc
    15781 ggcctcagat catggaaccg gcgtggaatc cggagtatca tcgcttgttg aaggcgtttg
    15841 aggcgcgcac cggcatcggc ggcctgctca atacctcctt caatctgcac ggctacccga
    15901 tcgtgaacac gccggccgaa gccctcgacg tgatgcgccg gtcggggttg aactacctga
    15961 tcctggggga cctgtgggtc gaaaagctgt ccgacgaatc gcgcgacgga ggccgccatg
    16021 ccgtcgctcc gtgaacagcc ctcgattgcc gcggtggagc gactgatgac gcggaccggc
    16081 gcggtcgata tcaccgtcgt catcccgacg ctgaacgagg cgggaaatat cggcgaactg
    16141 gtccgccggg cgcgcgcgat gctcgacggg atgggcgtct cgcacgaaat cctggcgatc
    16201 gacggcggtt cgaccgacgg cacctgcgag gaggccgagc aggccggcgc cgtcgccatc
    16261 cgcgagggag gccacaccta cggcgcggcg ctgcggaccg gcttcgccca cgcgcgcggc
    16321 gaattcatca tcacgatgga ctcggacctt tcgcacgagc cggaattctt cgagcagttg
    16381 tggcaggccc gccgccgcgc cgacatcgtc atcgcctcgc gctacgtcgc cggcgggaag
    16441 gccgacatgg cgccgttccg ctccgtcctg agccgcatcc tcaactttgt ctacacgcgg
    16501 attctccgca tcccggtcca tgacatctcc agcggcttcc gactttaccg ccgcgccgtg
    16561 atggaacgga ccgaaacaac ggcccatgat ttcgatgttc tggaggaagt cttgatcaag
    16621 ctctacgtgc aggggcacgc ggtcgccgaa gtcccctttc attaccgccc ccggaagcat
    16681 gggcgcagcc acgccaagct ggtccggttt gcgctcgcct acctgaaaac gctctggcgg
    16741 atggcgcggc tgcgctacgg gacggccggc ggccgcgacg cccgggagcg ccgcccgtga
    16801 cggctcgtga actggtggcc gccggccgca agaagacgcc ccacctggtc ctgtggatga
    16861 tcgtcggcct ggccggcggc gtcttcgccg gcctttactg gaacgttctc tatctgcttc
    16921 tggaaggctc ccagcggatc agcggctggg gcctgctgct gctgatgccg gcggcggggc
    16981 tggcgatcgg ctggatcatc acccggcttg gcgatccggg tgaaatttcc ctcattgtcg
    17041 acaacatcca cttccgcggc gggcggcttg atgtccgcaa caatccgtcg atgatcctct
    17101 cctcgctgct cagcatcgcc agcggcggca gcgccggtcc cgaggcgccg atggtccagg
    17161 tcaccggctc gttcggcacg tggttcgccg atcgccttgg gctgcgcggc gaggcgctgc
    17221 gcaccctctc aatcgcgggg atggcggccg gcttcaccgc gctgttcggc gccccgctcg
    17281 gcggcgcgat cttcgccctc gaaatcctcc accaccagca cgtcgtcgag tatttcgagg
    17341 cgctgctgcc ggcggtcatc gccagttgct cgggctacgt cgttttcgcc gccgtcaccc
    17401 acctcggctt tggccccacc tgggtctttc cattattgac ggccaacgaa atcgccgact
    17461 tcgggctgat cctgctcttc ggcttcctcg gcgccgcctg cggctggctg tttgtcaccg
    17521 tgtttcgcgg catcggccgc acgttcgagc gcatccccgg cccgttcttc ctgaaaaccg
    17581 gctttgccgg cctgctgctg gggacgattg ccttcgtcat gccgctgacc cgcttctttg
    17641 gtcacgacga agtcgcccgc gtcgtcaccg agccgttgac cgtccccgcc ctgggcgcgc
    17701 tcattctttt gaaaatgatc gcgatcagtg tcacgatcgc cggcggctgg cgcggcggca
    17761 tcatcatccc gctgttcttc atcggggcct gcctgggcaa actggcgatc ctggtgctgc
    17821 cggggcagat caaccccgcc ctggccatgg ttggcgtgat ggccggcatc aacgcctcgg
    17881 tcacgcgcac gccgatcagc accgccatcc tgctggccaa gctgaccggc accggcgcgt
    17941 tgacgccgat cctcttcgcc agcctggccg gatttctcct cgcgccgcgg acgccgctga
    18001 tccgcgcgca actcaaacgc cctccctcgg gcagcccttt gccctgaggc aagcgaggcc
    18061 aacggatgac agcccagcac gttctcgtca ccggcggcgc cggtttcatc ggctcgcaca
    18121 tcgtcgatgc cctgctcgaa cgcggccatc gcgtgcgcgt cctggacagc ctcgacccgc
    18181 aggtgcacgg gccgggcgcc gacggacccc ccgcgtatct caacccggcg gtggaattcg
    18241 tccgcggcga cgtgcgcgac gccgacgcgc tcgaccgcgc gctggacggc atcgaggtcg
    18301 tcttccatca ggccgccgcc gtcggcgtcg cccagtcgat gtacgccatc cgcgcctaca
    18361 ccgacaccaa cgtgatgggc acggccacgc tgctcgaact gatcgtcggc cgccaccgcg
    18421 accacgtgcg caagatgatc gtcgccagtt cgatgtcgat ctacggcgag gggaaatacc
    18481 tcgaccccga aacgaacgag ccggtgcgga tcgtcctgcg cgacatcccc cagatggagg
    18541 ctggccagtg ggagccggtc gtcccgggaa cccaccgtcc cgcgacgccg gtcccctgcg
    18601 acgagctgaa gccactcatg ccgacttcgg tttacgccgt caataagcgc gaccaggagg
    18661 aaatggtcct gacgaccggc ctggcctacg gcatccccgc cgtggcgctg cgttatttca
    18721 acgtgtacgg cccgcgccag gcgctgtcca atccctacac cggcgtcgcg gcgattttca
    18781 gttccgcctt gatgaacggc cgcggcccgc tcatcttcga ggacggccgg caatcgcgcg
    18841 atttcatcca cgtccgcgat atcgcccgcg ccaacgtcct cgcgatggat tccgacgcgg
    18901 ccgactatca ggcgatcaac atcggcaccg gccgcccgac ctcaatcctg caggtggcgc
    18961 gcctgctcct cgaacgcctg caccccgacc gccgcgacga tccccagctc cagccgcgca
    19021 tcgtcggcaa ataccgcgcc ggcgatatcc gccattgtta cgccgacatc gcccgcgccc
    19081 gcgcgctgct gggattcgag ccatcgatca cctacgaaca gggactcgat gaactgatcg
    19141 agtgggtgcg cacgcagcgc cccgaggaca agagcgagca ggcctacagg gaactggccg
    19201 accgcaagct ggtgtagggt cttcccgctg cgtcggcctt gcgagccgcc ggggcggacg
    19261 gtagtgtagg gcgagcaaca ttccgccgcc ggaggtttcc cccatggcgc ccgccgcttc
    19321 cgcccgccgc accccccgcc cgctgaccta tgctcaggcc ggggtggata ttgccgccgg
    19381 cgacgaactg gtcgatttcc tcaaggccgt caaccccgcc atcggcgggt tcagcggcct
    19441 gttctcgctt cccagcgggc tgcgccggcc ccggctggtc gcctcgaccg acggcgtcgg
    19501 caccaagctg ctcatcgccc aactcgccgg ccgccacgag acggtcggca tcgacctggt
    19561 ggcgatggtc gtcaatgacc tggtcgtttg cggcgcgcgg cccctgtttt ttctggatta
    19621 tttcgccacg ggccggctca agcagcacga agcgcgcgcc gtgctcaagg gggtgatcga
    19681 cggctgcgcc caggccaact gtccgctgct ggggggtgaa acggccgaaa tgcccggcat
    19741 gtacgccccc ggccactacg acctggccgg gttcgccgtc ggcgtggtcg aggccgaccg
    19801 ggtgatcgac ggccgcgccg tccgaagcgg cgacgtcctc ctcggcctgg gttccagcgg
    19861 cctgcattcc aacggattct cgctggtccg ccgcgtcctg ctgcccggcg gcgaaccggc
    19921 gcaaaccagg cgcgcgctgc gccgccggcc gtggcgaggc ggcccgacgc tcgccgacgc
    19981 gctgctcgag ccgacgcgga tctacgtcaa gccgatgctc gacctcgtcg gccggttccc
    20041 gatcaaggcg gcggcgcaca tcaccggcgg gggcatcgcc ggcaatctgg tccgtgtcct
    20101 gccgcccggc gtccgcgcct ggatcgacct cgatgcctgg cggccgcagc cgatcttcga
    20161 tctgatcgcg cgcgagggcc cggtcgaaga gaccgagatg ttccgcgcgt tcaacatggg
    20221 gatcggcatg atcctggtcg tcgcccccga tgtggctggc cgcgtcgcgg cccgcgccga
    20281 gcggctcggc gaaacatgcc ggccgatcgg ctggatcgac tccgccccgc gccggaaggc
    20341 tcccccgatc gtcgaacttg tgcggaggag ccagcagtag atcgtcaacg gattcgacct
    20401 acccgacggt gtgggatcgg cgccctcgcc ggtcctcccc cgtatcctgt cagggatgcc
    20461 agacgtcgcc ccggtcccga tttgccgaag gaaagatccg acgacgaatc gcgaagcgcc
    20521 cttacggcgc cgccgctgtc cccggcgatt cgcccaattc ctccagcagc gactggccga
    20581 gcgctcgcgt ttcctccccc gcatacaggc tgcgcgcgat cagtgaagcc cgctcccggc
    20641 ggcccgccgc gatgtagacc tcggccattt cctgggcgac ctgcggcgtc aggaagcccg
    20701 gcccagcctg ctgcgccgcc ccccagagct gatccgccag ccgcacggcc tcggcctgat
    20761 tcccctccag gcgcgcgagg cggaaccgcg cccgcgacag ctgcggcagg cggtgcgcca
    20821 gcgccggata atcccgctcg aactcccgca gcagccggcg cgcctggtcc gcgtgaccct
    20881 gatccagcgc ggcgcccgtc agattgacat agtcgttcag ccgcggcttg gcggcttccg
    20941 gacgccacca cggccccagc aaaataacca gcgccgcgaa cgcgccgccg gcggccagca
    21001 gcgggccgta gcgccgcgtc cgccactggt gcagacacca ctccgccgtc agcccgaccc
    21061 agatcgccac cgggatgaag agcggaatgc gatagcgtcc ggtcggcagg atcatgatca
    21121 gcgtcgcgga gaacaccccg gcgaagatca gcaacaggct ccagcggcgc caccaccgca
    21181 gcgagacggc cgccccgaca aagcccagcg gcaccagcca gacccactgc agcgggttca
    21241 ggccgatcgt cgggtagagc cggcgcgcga gcgggtagct caggttgtcc gccaggtcgg
    21301 gcgaggcaaa gaagaagtac gccttgcgga agaggttgag caagatgtgg ccgggggcgt
    21361 tgcgcagatc aacgaaccac tcgcgccagt agatgctcat gtcggcgccc gccagcgcgt
    21421 gcacctcctg aaaccgcggc gtttccgtaa agtagcccac cgcgtcggcc gcattgccca
    21481 ggtagaacag cacggtcccg ttcttcatcc ccatttccca ggcgccggtg acggcgcggt
    21541 tgcgcagtag aaacaggaac atcatcaggg cgacccccgc gcacagcgcg acggcggccg
    21601 gccagcccgc gcgcgcccac gattcaggcc ggcgcaagcc ctccgggcgg gcgaacagca
    21661 gccagacggc gagcaccggc gccagcgcca gcgcgttgcc gcgcgccagc gttcccagaa
    21721 agatgcagct ccccgccaga aacgccagcg cccagggagc gcgcgcgccc agctcaatcc
    21781 ttcgcgccga ccagagggtc acggcgtaga gcatgatcag caggccttcg tgcagcaggc
    21841 cctgctcgta gaacagccac ggcgccagcc cggcccagac gatgccggcc gtccacgcgc
    21901 cgacccggcc gagccaggtg ccggcgagcc ggaacagcaa cccgcaggtc agcgcgccga
    21961 tcagcgcctg gatcagatgg agcgcccaga aattctgccc gccacagatc ttgtagacga
    22021 ttccggcgaa atacgcgtaa atcggcgcgt agtagaacag cccccccttg cgcaccagcc
    22081 agtcccccgc caggatctcc ttcgcccagt cctgatagac gttctggtcg tagcccggca
    22141 tcgtgtaggc aaacagcgga ttgccgcgat agagcaacca gaccgtgacg agccgcagcg
    22201 cgagcgcggc cgcgatcagg cccagcacgg ccgtccacgg cggccgccac ggccgcggtt
    22261 catagggcgc ggcggcggcc cgcgtcgccg ccgccggcgc ggccggtttc cctttggccg
    22321 atttctgttg cgaacgggcg gagcgacgct tactcatggg ggttcatccg gcgcggcggc
    22381 ggggtttttg cgcgattatg actttgcccg gcgcgacttg ccagcccgga tcagacgccg
    22441 ttgccttggt gcgcttcgca tccgcctgga atcctcgctg ccaacgccgg aggccgtcgt
    22501 gctcctcgaa ctcgacaacg tgacggtccg ctacggcgcc cgcaaggcga tcgaccaggt
    22561 cagcctgcgg ctcgaaagcc gggcactggg cctgctgggg ccgaacgggg ccggcaaatc
    22621 gacgctgatc cgcgcgttgc tgggtctgca accgctcgcc ggcggcacgg cccgcctcga
    22681 gggcctggac atccgccgcc agggcaagcg catccgcgag atcgtcggct acatgcccga
    22741 gcacgagagc tacctcggct cgatgaccgt cgtccgcttt ctccggttca tgggcgagct
    22801 gtgcggcctg ccccccggcg cggcgatgga gcgcgcccac gaagtgctgt tctacgtcgg
    22861 cctgggcgaa gtccgctacc gcaagatgga gggcttcagc tacggcatgc agcagaaggt
    22921 gcgcctcgcc caggcgctga tccacggccc ccgcgtgctg atcctcgatg agccgaccaa
    22981 cggcctcgac ccggccgcgc gcgacgacat gctgcgcctg attcaggaca tggtccgcaa
    23041 cagcggctct cgcctgatca tcagttccca tctgctgaag gacatcgagt cctgctgcga
    23101 ggaggtggtc gtcctgaaca agggccgcgt gacggccgcg gggaacatcg ccgcgatgaa
    23161 gcagaccgac gaacggcggt tcgagctgcg gatcaagggt ccgctcgatc cgttcgtcgc
    23221 cgcgctgtcc gagctgggct gccaatgccg gctcggcaag cgcgaggcgt tgcaggtggt
    23281 gctgcccgag cacctgccgc cgcgccgcat ttttgaagtc gcccgcggcc agcgcgttca
    23341 gatccggcac tactacgccc gcaaggatac gctggaggat gtattcctga aagccctgga
    23401 ggacgacgcc ggcgagcggg aggtgatggc cgatgccggt ctatgacctc ggataccgcg
    23461 tgtgggaggg gaaacggcgc gggccgctgc tgcgctggct cgcgatcccc cgctttgcct
    23521 atatggacct ggcggggcgc aaggcggcga tctacgtctt ttcgatggcc tggatgccgt
    23581 tcctgctcct ggccggctac atctatctgc tggtcaatta ccgcttcctg cagacgctcg
    23641 gcatcccggc ccggatcctc tttccgatcg acgcggcgct gtttaatctg ctgctcgacc
    23701 tgcaactctt cttctgtttc atcttcgcgg tcatgctcag cccggacctg atcgcgcgcg
    23761 acctgcatca ccgcgcgatc gtcctgtact tcgtcaagcc gatcagccgt tgggaatacc
    23821 tcctgggcaa attcgcgatc ctgatgtccc tctgcctgct gctgacctgg gtccagccgg
    23881 cgctgctgtt cctcattcaa tgggcggtgc ggcccgagga tccgacctgg cgcacgacgc
    23941 tctggcgcgc cgatttgggc gtgctgcgcg ccattacgct ctacggctgg accgcctcgg
    24001 ccggcgtgac gctcctcgcc ctggcgctgt cggccctcgt gcgcaacccg cgacatgtcg
    24061 ccacgacgct cctggtcttc ccggtcgggg cgtggatggt cgccggagtt ctggcggaag
    24121 tcctcggcac ggcgtttcgc gtcctctcgc cgctccatgc gatgcgcgaa ctggggacct
    24181 ggttcttcgg cttgcccacc gacgcgcggg cgccggtctg ggccgcctgg ctgagctggg
    24241 gcggattggc cgccctctgc gcgctgatcc tcgtctggcg cctgcgccgg gcggcccgcg
    24301 gcggcgtcgc atgacgcacg gccccgtcac gatcgagctg cgcgacgtct cgcgctggta
    24361 cggcgaggtg ctcggcgtca accgcgtcac cgccgatctg cgcgccggcc tgacggggct
    24421 ggtcggtccc aacggctcgg gcaaatccac gctgatgaac ctgatctgcg gcttgctgcg
    24481 ccccggccgc ggcgagatcc gcatcctcgg tcaacccgtc tggaacaatc cggagctgcg
    24541 gcgcgtgctc ggttactgca cgcagaacga tcacttctac gaatccttca gtgggcgcga
    24601 gttcgtcgca tcgttgctgc gcctgcacgg gcacggcctg gactgggccc gccgcaccgc
    24661 cgccgactgg ctcgaacgcg tcggcctggg accgcacatg gaccggcgct tgcgcaccta
    24721 cagcaagggg atgcgccagc gcgcgaagat cgccctggcg ctcgcccacc agccgcgcgt
    24781 cctcgtgctc gatgaaccgt tcaatggcct ggaccccgtc ggccggcgcg aaatgatgca
    24841 actgctccag gagtttgccg ccgagcagcg cacgatcgtc ctctccagcc acatcctgca
    24901 cgaaatcgag cagatgaccg acaacatcct gatgatgagc aacggctatg tcgtggcgga
    24961 gggcgaagtc ggccaggtgc gcgacctgct gaccgaccac ccgttccagg ttttcatccg
    25021 ctgcgacgac ccgcgccggc ttggcgccct cctgctgcgc gaacccggag tgctgagcgt
    25081 ccaggtggag ggggaacgtt cgctgacgct ggcgacccgc gcgccggacg ccttttacct
    25141 gcggctgaac gaactgatcc tcgatcacgg gatcgacgtg gacatcgtca cgctcgccga
    25201 cgaaaacgtc cagtcgatct accgctatct cgcgggccgg gagcaccact gatgccgccg
    25261 atggcgaccc cgacgccgaa taaccgcacg gccagcgcgc tgttggcctt ctggggggca
    25321 gtccgcctgt cgctggcccg gctgggccgc acgacctcca tgctggccgt gatcttcatg
    25381 gcgctgctgc cgctgctgat tgtggtgctg atcgccgcgg cgacctggca cgagccgatt
    25441 ttccatcagc ccgccgccgc gcagcgcgtc tatcagacca tcctgcgcct ggtcggcctc
    25501 catttcgttg cctttttcat tgcctacatc cttggccagg ccgtcgtccg gcaggacctg
    25561 gaggacgaaa cgctgcatta cctcttcctt cagccgatgc cgcgctggct tctgttcgcc
    25621 gcgcgcttcg tcgccttttt cctgctggcc ggcgccgccg tcgtcctcgg catgtggctc
    25681 tgctggctgg cggcgacggt cccgtggatg cgcccggcgg atctgtttga tctgctgcgc
    25741 gagcagggcc ggctggccgg cctgctgcgc gaagcgggcg tcctgctgct ggcggtgctg
    25801 ttctacggcg cggcgatgat cgccgtcggc tcctttttcc gctcgccgtt tttcgcgggg
    25861 ttgctgctgg gttgggaagc cggcgtctat tacctgccgc gcgcgctcaa gtactggacc
    25921 ggctcgcatt atttccattc gctgctgccc gacgggggcg ccgagcgacg gctctttgaa
    25981 ctcatcggcg agccggccgg cgtcggcttg tgcctgacgc tgatcccggc ggcgtcgatg
    26041 gtgctgctcg ccattggcgc cgcggccttc cacctgcgcg aatgccagta cggcaagacc
    26101 aactgacctg ccaaaccacc ttcacctcca tcaggtcgcc tccgtccctt caacccgctg
    26161 cgtgccgttc acgctctccg caccgtcgtt ccgcgcgccg cacgattcgc agtgcggcag
    26221 caggctgtgg cgcagccacc cgcaggcgcc gcagcgctca tacagcggcg tgccgcaggc
    26281 ggggcaggtg tagggcgtgt cctcgagcgg cggggtttcg ccccccgccg ccagcgcccg
    26341 cggcccgccg cgcgtccaga ccgcgaagcg cagcggcccg cggccgatcg ggtagtcgca
    26401 caccgggcag tgctggctct tgtagccctc gcggtagcgc ttgagcaggt cgagcgggcg
    26461 cggccgcgcc accgcgcgca acaggcggac cagcagccca atcgtcacga cgagcgcggc
    26521 ggcgatggcg atgtacttga agaactcccg cgggaagtaa tcccacatca cgcccgcaac
    26581 cttccagaac gcggccacgc acagcgcgat gtagatcgga cgatagatgc tcgcgcgccg
    26641 gcgcacggtc agccaggccg ccgccagcag caccggcacg atgaacgcca gcttgagcga
    26701 cccgaccagc agccggtggc gggtcagttc cgcctcgtat tgctcccgcg ccggctgcag
    26761 gatctcctcg atccgctccg caatcgcgtt ctgctcatcc tgcgcggcgt gctgcgcttc
    26821 gttcagcccg gcgatttcgt tgttgatctg ttcaaactgg ttctgggcct ccaggaaccg
    26881 ctcctggctg agcagcagcg cttcctggtc ggggggcgcg ccctcgcccc gttcaagata
    26941 aagctggtgc agcttgatca tctgatccat cagcgagcgg gcgttgtcca tcccttcctg
    27001 gagcgtcgcc tgccgctcgc gtcggcgggc gatctcgcgg tcgatccgtt gcagctcctt
    27061 gcccagcgcg gcctgccgct cggtcagatc cgcgggcgtg gcggcgtcca tcagggcgtt
    27121 gtagtcgggg ggattcaggt cgccgatgtc gtcgagcacg aagcccaaca gccagatgaa
    27181 gagaaacgtg agcaggatgg cgaggagcac gatgccggcg cgcagggcga acgggatgcg
    27241 gcgcgggcga acggacgaac cggatgagtc ggccatggcg gcagcctccg tgagggatgg
    27301 ccaaggttac cggatgtaat gttcacatat caacagaatt cgcgcgaccg ttttcctcgt
    27361 tgtcccggcg ccggtcgcca tggaaaatat cctcatgcgc cttgacagcc agcttgccgt
    27421 cgtccacgcc tggtttcccc cggcatgaat ccgccgcgcg cggccgtcct gccgggctgg
    27481 cactgcgccg cctgcggcgg gcaggtgcgc aaggacgacc gggtctgctc ccattgcggc
    27541 gcgcggctcg tccagcctgc cccgtggcac gagcaacccg tctggatcgc cgtcctgatc
    27601 ctgacgctgc tgggcgcgct cggcatcccg ctgatctggc gttcgcgcca gttgagccgc
    27661 ccggccaagt ggctctttac gctcgcgaca attctctata cgctgatcat catcctgctc
    27721 ctggtctgga tctcgatcct gctctgggag cacctgcaag ccttccagat gcagatggac
    27781 gacctgggga tgtgaggatc cccggtcccg tccgcgccac cgcccgccat gcgccttgtc
    27841 tcgctcaacg tcgcccgccc cgccgagatc ccctggcaag ggcggatgat tcgcaccggc
    27901 attttcaaag cgccggttga ggggccgcgc cgcgtctcgc gcctgcggat cgagggcgat
    27961 ctccacggcg acctctccgt ccacggcggc ctggacaagg ccgtctacgg ctatccgcgc
    28021 gagcattatg ccttctggcg ggcggagctg ggcgcggcgg acgactttcc cttcggccag
    28081 ttcggcgaaa acctgaccac cgaaggcctg ctggaacgcg acgcctgcct gggcgacgtc
    28141 taccgcatcg gcggcgcgct gctccagatc agtgaaccgc gccagccatg ctacaagctg
    28201 gcttaccgga tgggccgccc cggtttcgac gcccggtttc tcgccagcgg aaagctcggt
    28261 tactatttcc gcgtcctgga ggagggcgtg ttgcaggcgg gcgacgagat cgtccgggtt
    28321 gagccggatc cccagcgcgt cagcatgctg gagctcgcct ccctgctcta cctgggcggc
    28381 ggggacaagg atctgctgga gcgggccctg gcgatcgaca gcctctcgcc caagctgcgg
    28441 tcgcggctgg cacggcttga acggtgagcc cgctcggtgg gcaccgattg ttcggttcga
    28501 tatcgaagat tcgagagccg aggacgagag ccgagagccg agagccgaaa tctgggtcga
    28561 tcatcgccgg tcgaggacga ggacgagaga cgaccgctga ggaacggatt gcacgcgggg
    28621 gatcattccc gccggcaggg ccagccttgg tcgggcgtgg acacgggcgc ggagtctgct
    28681 aagatgagcc atcccctggt ttcgcgtccc ggtcgcggtt cttgaggccg ggcgcgtccc
    28741 ccgcggtctg gcgctcgccg tggggctccg gatgccgcat atctcgctgg agtttccctg
    28801 tgaaatacct gcccacctcg cgcatacccg cgttgctcct gctggttggc gtgctgacgg
    28861 ctctccccgt gctcggcccg gccgagccga tgatcgagga cgccgacaac cccgagaaca
    28921 acctgggcta cgtcacgccg cccgacctga cccgcgaagt cccgatgatc gaagtgatca
    28981 acgatccggt cgagggctac aaccgggcga tcttttcgtt caacaactgg ttcgccatgt
    29041 gggtgatgcg tccggtgacg aaggggtatc gcgcgatcgc accgttgcag gtccgtctca
    29101 gcatccgcaa attcggccac aacctggcct acccgctgcg cctggtgacg acgctgctgc
    29161 agggcaagtt caagggcgcc ggcgtggaga ccgcccgctt tctggtcaac acgacggtcg
    29221 gcctgctcgg gttttttgat cccgccggct ccaagctcgg cctgcggccc tatgacgagg
    29281 attttggcca gacgttcggc aagtggggtt ccggccccgg cttctacttc gtcctgccgt
    29341 tcctcgggcc gagcagcggg cgcgatacgc tcgcctggcc gttcgacgcc gtgctgaatc
    29401 ccgcctcctg ggccccgggc ctgggccagt tcctgatggt caacacgctc agcttctcgc
    29461 tgccgaccta cctgcgcctg atgcagaccg aaatggatcc ctacatcctc acgcgcgact
    29521 tcgcggcgct cgcccgcaaa cgcgaagtgc tcgattatcg cccgattgag acggagatcg
    29581 agccctcgcc gacgctgcag gcgatcttcc tcgacgtgcg cgatccgcgg tttcccgacg
    29641 ccggcgccac cgcccaggtg aaaatccctt ccaccggccg cgaacttccc tattcctatt
    29701 ggctgcagcc gacccccgcc ccgctgatct tcatcatggg cggcgtcggc aatcaccgcc
    29761 tttcggaatc ggccatggcg ttcgccgaaa tcgcctacac gcacgggctg tcagccgtca
    29821 cgctcagcag cgcgatgaac tgggagttca tggagcacgc cgccaccgtc agcgtgccgg
    29881 gctacacgcc ggtcgacgcg atggatgtct atcgcgcgat cgacgcgatc catcaggacc
    29941 tccagcagcg ccatggggcg aacatcaccg ccatcggcgt gatgggactc tcgctcggcg
    30001 cgctgcactc gctgtatctg tccgccttcg aaaacgagcg ccgcggcggg ctcgtgccga
    30061 tcgaccggtt cgtggccgtc aatccgccga tcgacctgtt ctattcgatg cgcaagctgg
    30121 acgaatacta caacgcgcca ctgcaatggc ccgaggccga acgcgcggcg cgcatccaga
    30181 acacgctgtt caaggccgtc cagttgactg agatggacct gacgccgaag accgacctgc
    30241 cgttcgatgc gatcgaatcg caatacctga ttggtttgaa tttccgcctg atcctgcgcg
    30301 acatcatcta caccagccag cggcgcaaca acatgggggt cctcgccacg ccgctcgaca
    30361 cggtcgcgcg ccaggccgtc tatgacgaga tctcgcagtt ttcctatctg gaatacgtcg
    30421 accggttcgt cgtgccgtac tatctgtcgc acaacgaact ggcggcgacg gccgacgagc
    30481 tggtcagccg ctccaacctg cgctggatcg aggattcgct cgccggcgac gaggacgtgc
    30541 gggtctacct gaatgaggat gacttcatca tcgatcggga ggacatcccg tggctgcgct
    30601 cggtgctggg cggccggctg acggtgttcg aggggggcgg gcacctgggc aatctgtatc
    30661 gccccgaagt gcaggccgag ctggtctctt cgctggttga gctgcgcgac aacccgcccc
    30721 ggcgcgatgc ggcggaggac gcggcccccg cggtcgaccg cgagccggtc ttgctgccgg
    30781 cggtcagcct gccggcgccg gcggacgcgg cgctgtaggg cttgtcattc accaaatcgc
    30841 aagctgcttg aggcttccag ttcagtgcag gcggtttgaa cttattgccc ggtccagacc
    30901 gcggagcgga cgaccggaat aaagcgcagg ccgacggccg cagactggaa ccctgcgtag
    30961 ccgtccccga tgtatcaaac ccctggaaag ggtgaccgga gccgtcctga atttgggaat
    31021 gacaagcgct gcaggagagg gacgcgccgc tattcgttgt ggtcgaatgg ttcgttgacc
    31081 gagcaggtcg tgtcgtcgga aacggcgccg atcgtgtaga cgccgctgct ggggcagctc
    31141 ggttcgctct tgaggtagcc ggagccgtcg ggttggacca gatcatccca gccggggacg
    31201 gcgtcggggg gttgcttgaa atcaagcgcc cagacttcct tggcgccttc gattttctga
    31261 agattctcct gacagacgcg ctgctgcgac agaccgcgct gccggatcca ggtcgggccg
    31321 gcgatggcga ggatcaccgc gaggatcgcc acgacgatca tgatttcgat cagcgtgaag
    31381 ccggcgatcg cgctccgccc gaagcggatg catcgtcgat gactgcgggc gtgcggctgg
    31441 ctggccatcg tcgtatccct catgttggcg gggttcgacc gggtcgggcg ccgacccgcg
    31501 agggcggggt tggcggcggc cgccgcggcc ggcgcaaaat caggttgcgg caaaagagaa
    31561 gggcgatagt tgccgggccg tggcccctat cgcccttcat cagcctagct gcttggagag
    31621 gcgtccatcg gttgattcgg cgttcagggg ccggatcaga tggcgtcgag cgcgtggtcg
    31681 ccgccaacgc tgcaggtcgg gtcggtgccg accgcgccga cggtgtaggt gccgctctcg
    31741 gggcactccg gctcgacctt caggtagctg gtcgtcggat cgcccgtgac caggtcgctc
    31801 atcgcgacgg cggcgccggg cgccagatcg ttctccagcg cgtgctgttc cttcgcgccg
    31861 tcgattttcg tcaggttttc ctggcaggca cggttacgcg actgggttct ggcgcgaaca
    31921 aagccgggga ccgcgatcgc gatcaggatg ccgatgatcg ccacgacgat catgatttca
    31981 accagggtga aacccttgct ggtcttgcgg aaaaacttca tgtctccacc tccgtgttat
    32041 ttgtcgagcc cactggatct cggggtctcg tcagctccac cgctctgcct tccgctgtca
    32101 gcaaccgcgg tgccacatct tccaaattct aatgagattt actgcccctc cgaaaaaatt
    32161 gcctgatttc attgtatttc caaggtcttt cccgtttcgt cgccgctccc gcggtcgccg
    32221 ccgtcgtgcc gttcactcgt tgcagcctcc gcgcgggcgg gtaatgggta acccgttacc
    32281 cgcggcgacg ggaatcagct tacccatcgc gcctctcacg aaaccgccgc cgccggcagc
    32341 ggctcggcct caacaagatg ctggaaaatc ggcgtgctca ggctgtaggc gttgttgccc
    32401 aggacaatct ggcgcccgtg ccggttctcg acgttgacga tcagcggcgc cttcaggttg
    32461 gccgtcatcc ggatcggcgc gtcgtgcggc acgttgacga tgctcatgat cgcgcagccc
    32521 tcgacggcgt cggtgccgat catccgggcg gtgtccaggt cgatttcgag atgatacccg
    32581 gcatcgatga acagtggatc gacgatgatg aaggcgagcg ccggattttc caccgactgg
    32641 agccacttga acgggctgcc gtcggtgtcg tgttcgagca ggatgtagcg ctgctcgtcg
    32701 gggaagccca gaatcccctc cggaaaggtc atgacgacct cttcgggaaa ctcgatttca
    32761 ccgaagcgcg tggtgtggaa tcgcatggtt acgccttccc gttgccattg tgggacagat
    32821 tgcgcggacg ctgtgtcagg ctttgctgat cgcgccagat ggaggtgagc acctcggagc
    32881 tgtcggggct ggagagcgcc gccagcgcgt tctgctcgcg gatcagatcg tagatttcct
    32941 cgcggtgcac actgatgttg cgcggcgcgt cgacgcccac gcgcacctgg ttgccgcgaa
    33001 tttcgaggat cttcagggtg atcgagtcac cgatccgaat cccctcgccc gacttccttg
    33061 tcagtatcag catggttcgc aaccttcctg gtccacgcgc tgatgcgcta tgcaacggcc
    33121 gccttttcca gtactctacg gtcaaaaagg tccgaagctt tagcgcatcg ccgaccgact
    33181 tcgtatcggc cgaagggccg ccccggtcgc cggttcctcc ggctgggcgg tccgtctgta
    33241 atctcatcgg accggacgcg gcgagggttt aacgctaatt gacctggcgc ggcggaatcg
    33301 ccaaccgagg ggaaccgacc gaaattatgc agaaacgcga aatcgccggg actcaaagaa
    33361 ggagagacga tcaatgattc tgtggtagtg cggggggctt ggagcgggcc gccggtcgcc
    33421 gttgcgaacg ggcgatggga tccgcttacg cttgggcgcc gctcatcaag gcgagcagcc
    33481 ggcgggtcag ggcgacgccg tcccaggcgt gacgcccctc cgggatatgc tccagcgcga
    33541 agctgatggc cagcgagagg gggacgagct gcggttcgcc gccgaagagc tcggcgtgca
    33601 gccgggccag agcttcggaa aggcggaccg aaacgccgac ccggcggatc gggttggcgg
    33661 ccggatcggc gcaatcggcg gcgggcgagg tgacgagata ctcgcggcag accgacgggc
    33721 gcccgccatg caccgagcag ctctcctcct ccaggaacgg gcagggaatc cccagcagga
    33781 aatagcgcgc ggccagggcg cgcgcggctt cgtccgattc gggcgcgcgc agcagggctt
    33841 cgctcagccc ctcggcctcc agccgcgagg cggccgcctc gaaccgctcc aggtagcgcg
    33901 cctgccgctc ggcggggagg tgcatcagca ggtcggccag catgtaggct tcggggggcg
    33961 agacgggggc cagttgccgg cagcaggcgc cgcagccggg cccgcacgag atcacgcgcc
    34021 ccgcgcgctg ctcgcggcgg gtggccagct cgaccaggcg gtccaccagc ggcagcgtgc
    34081 tgcgcgccag ctcggccagg cgcatcggcc cgggcgggat ggcgagcatg ccgcgcaggt
    34141 tgccatcggg cgtgctcaga tcgaactcga gcagacgcgg cggggatggg gcgccctggg
    34201 ttccggtttc ggtcatcgcg gccggcccgc gtgcatcggg tcctcagcca tgagccgggg
    34261 tttccacggg gatgcccgcc gcgctcaggt agtagaaccc cttgttttcc acggcatact
    34321 tgatgaccgg ggtgcccgag atgaggaaca ggagttccag cgggagatca ttttgcgcga
    34381 tctcggccgg cagcgggacg aggccgtgct gctcgtcgta gaattgccag tcgctcccct
    34441 gaaaccggat gagcagggcg ggatcgccct tggaggagac gccgacgacg gcgcggtcga
    34501 ccttctgcat cggttcgcgc ggcaccagca ggatgtcgct tgcctcgaac tggccggcgg
    34561 cgctgcgctt ccagcaatac aggcccagct cgcgcgccgc ctcggggccc ggttcaagta
    34621 ccaggagggt cccgtggagc gtgtcgtcgg cggccagggc ggtggcgccg ccgtggaatt
    34681 cgccatcgaa ctgcgccagc ttcttcactt cggagggctc gccatcggcc gaccaatcga
    34741 ccgcctgcag gaggaaattc tccccttgcg tcgggagcac gaaataaatc tgcgccgacc
    34801 cgttgctggg gaacgccgct tccatccagc gggggcgcag ccggccgagc atcacgttgg
    34861 cgcccggttc ggcgtcgccc cgggtcgtca gatgcaccgc ctgaaagcgc gagatatccc
    34921 cttcgacgtt gccctgccac aggaagaagc gcccttcgag cccggcgacg cccggctggt
    34981 ggttgagcgg cggaacgatc tgtttgggga ccggactgag cgggagcgtc ttgatgtcga
    35041 gctgctcggt cagatcgtcc acccggccga aacgcagctc atccccctcg aaccaggcga
    35101 tccactggcg atccgaagcc ccgccattga tcgggaccga aaggacgggc tcggccgacg
    35161 ggccgatctg gcccagcgac tgcagatgga cggtcttggg cttgttgatc aagtcataac
    35221 gcgaacgggt gagactgccg ggcggggtgg cgacgttgac ccagacggag ttgaagatcg
    35281 cggccggccc gcccgacatg ctgaccgtgt gggggtgccg gggccgcatc gcgcggatcg
    35341 tgacgtgaac cggttcggaa aaggcccgcc cgactttgcc gttccactcc cacagcgccg
    35401 agatctcata ctcgcccggc tcctggaact tgacgaacgt cagcagacga tgggtgcccg
    35461 aaagcgactg gccgggcagg agcggcgagg ggacctcctc gatgaaaagg ttgggatcgc
    35521 gccggtaaat ccggtccagc ccctgttcga tgctgaccac gcggagggcg gggagcgtcc
    35581 agcagtcggc cggattcatc gtcatgaccg actcgcggcc gacattggtg atcgtgacct
    35641 cgaagcgggt gttttcgtca aaggccagat cggtcttttc gacggtgagt tcaactttga
    35701 ggctcatgac gcctgtgctc cggagactag aatttggcga tatcgagacc ctgcaacagg
    35761 gcgagcgtct tgccgcagta ttggccttcg cagaagttgt agctcatcac gcaccagtcg
    35821 gcggcgtcgt ggcgcgcggc gatgcccccg ccgaacgggg atggctggtg cacggcgtac
    35881 agcgaatgac ccagctcgtg gcacgtattg gacgagacgt tgtatgggaa gctgggctga
    35941 ttgctgtaaa tgcgctcgcc ataccacaga taggcgccgc ggaagtgcag cgacacgccg
    36001 ctggtcatgt gcgggccatt cgtcgggccc cgcagcgcgc cggaagtcac gtcccagatg
    36061 cagcccatcg ggtactgaat gaacgtcaag ccggggagcg atcccttctt gctgtagtag
    36121 gagagcatgc cgttgaggaa ctcgttgagc accatcgaca tcgtgtcgcg cggatcgttg
    36181 cccgcggccg ggggaatcgg cccgatcttc tgggcggggg tcacgccggg gagggcgtcg
    36241 tattcggccg gttcgcggat attgatcgta aacggcgtcc gagcgtcgaa gaagaatagt
    36301 ttgtccaggt cccagttgac gcccatcgcc ccctggaagt tcttggcgta ggccacgccg
    36361 gcgtcgaagg cggcgcgctg ggtcgccgcg tcgggctcct cggggatcgc gacgttggga
    36421 tcgatgatga attcgcacca ggccttggca tactgccggg ccggaccttc ccagttgccg
    36481 gcgccgaccg gcccgttgtc gcgcagtccg gtgggcgtca tatcggcctg cttcatcccc
    36541 acataggtca ggtcctgctt gatgagccca caacgaagat agatcgtctc cttgttcaga
    36601 ttatacggcg ggccggcata cgcggcttcg atggcggccg ggaagttggc catcgccgga
    36661 tccttgcgga agatgtagcg gctcagccgg atcgtgcgcc agacgaccat cgtgccggtg
    36721 gtgatggcga ccgcctgatc gtc
//
LOCUS       DAOPCO010000010        35652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1349_length_35652_cov_5.718291, whole genome shotgun sequence.
ACCESSION   DAOPCO010000010 DAOPCO010000000
VERSION     DAOPCO010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 35652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35652
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1349_length_35652_cov_5.718291"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PLS90_01675"
     CDS             <1..923
                     /locus_tag="PLS90_01675"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPK01143.1"
                     /translation="AVKKEADAALKAHGLIKKKVRFEDEAVDTIIQRYTREAGVRNLE
                     REIATLCRKTARRLIAGGEESAPRITPQLVRALLGPERHHDLQIHDKTEVGVATGLAW
                     TDAGGEILPTETTTMPGKGNLQLTGKLGEVMKESGQAALSYLRSRSGQYGLAPDFHKE
                     LDIHVHIPEGAIPKDGPSAGVTLVTSMYSALTGRPVRQDVAMTGEITLRGKVLKIGGL
                     KEKVIAAHRNHLRTVLIPADNEPELEEIAPEILRELDIRPVRTVDEVLALALADEPAR
                     PDKSGGKPRRGQSGRGAGRRQPARGLGINA"
     gene            complement(983..1540)
                     /locus_tag="PLS90_01680"
     CDS             complement(983..1540)
                     /locus_tag="PLS90_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01144.1"
                     /translation="MIRSNHPLRVVFRRALGFGFDYAGIRDSDMARYLEENVMVEFIH
                     VDRIHRFRRADGRPVEAIADMLVALRESEQARAIPERDRHKYIGDYTLFMTGLFPELL
                     GQIRGDSTDHIVGSLIMSGDVFAWYVAEGRRSYGIAARFGERIFDELARNYVSYADAL
                     RLARNYLDTLKDRSLEGFTDGALAS"
     gene            1877..3181
                     /locus_tag="PLS90_01685"
     CDS             1877..3181
                     /locus_tag="PLS90_01685"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPK01145.1"
                     /translation="MIKPLVTGLIGFGNIGSGVLRAMAERAEELKARLPRPIVLKSIA
                     DKDTTTRRDAPYHKDQLHGDAAVVLEDPEIEAVMELIGGVEPARTFVERALRAGKHVV
                     TANKALLATHGPELLALAAENGVGLLYEAAVGGGIPIIRTLQQGLSANRLTAVRGILN
                     GTCNYILTRMAEAGLPFDQALTEAQEFGYAEPDPTYDIEGYDTAHKIAILASLAFGMD
                     IRFDDVFVHGITDIHPVDIKHARELGYAIKLLGIAQRHASDGSVIVRVHPTLVPLASP
                     LGMVGGVFNSILVEGTPIGPTVYYGRGAGPEATSSAILSDLMALASDTHGFNAARDAR
                     LTVRVGERTLRPIDQLRTNYYIRFSMVDRPGAMAEIGRALAEHNVSIESMIQHRVELA
                     QGMATITIVTHEALEKDVQGAIAAVEASEKSAGKAFVLRVEE"
     gene            3260..4423
                     /locus_tag="PLS90_01690"
     CDS             3260..4423
                     /locus_tag="PLS90_01690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01146.1"
                     /translation="MRITLLANPDNLHVQRWIEYLAGRGHALTLITDPFTRARPAACE
                     IHVIRWNPLTKLLAFRLTPRPLGNDRWKHLHARPLVRRSRPDVVHGFEAFNWGLATAL
                     AGPYPRVLTPWGNDVFEIPGRWPLARRMIGRALRSVDAISTNDETLAPYLAREFGVDP
                     ARVHGFTWGVDPGLFHAELPTEAADWRRRLEIPAGAPVILSPRNFHPYWGSEQICGAI
                     PRVLRQVPAAVFVILKGLGGDAAAFAEARRRLAAEGHGAALRWLAEPLAPREMAALFN
                     LADVFISVPKTDLLAQTILEGMACGCFPVLGDRPAYRKHLRPGENGLLVEDETPQALA
                     AALLVALANDTLRANARHENPRLIAAVENWQLNAQRMEAVYQTAIERFGRSAG"
     gene            complement(4424..7462)
                     /gene="uvrA"
                     /locus_tag="PLS90_01695"
     CDS             complement(4424..7462)
                     /gene="uvrA"
                     /locus_tag="PLS90_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK01147.1"
                     /translation="MSKPERLRNIVVTGAAEHNLKHINVTIPRQTLTVVTGLSGSGKS
                     SLAFDTIYAEGQRRYVESLSSYARQFLDQMHKPHVEHIEGLSPAISIEQKTVSRNPRS
                     TVGTVTEIYDYLRVLYANIGSPHCPVCGRPLQQQTVQEIVDKILDWPEGTKILVMAPV
                     VRGRKGEYKQLFEQAAREGFQRVKIDAEIHLVDEPISLNKRLKHDISIVIDRLVVTPK
                     IRQRLTDSVETALKKADGLALIERLAGPGDQPGEEITFSERMACPVHGPQIVELSPRM
                     FSFNSPFGACPRCNGLGTLPEVDPRRIVPNEALSIAEGAVLPWAGYFRNGAAGRAAEA
                     DIGDDPRPVAKPGRRPAAPARADDEERGEGWGKQYVLALLKHLRINPDKPWRSLTQRQ
                     RDAILFGTGQEPVPVRFVMGTGSRFETNKPFEGLVPRIQRRLRETTSEDILEHLRGYY
                     SDQPCPACAGARLKPGSLAVTIKGRNISEFCGQTVREALAFLDGARWSKRELAIGGQA
                     IKEVRDRLQFMVNVGLDYLSLDRAAGTLSGGEGQRIRLATQIGSQLVGVLYILDEPSI
                     GLHHRDNGRLLDMLKKLRDLGNTLVVVEHDETTIREADYVIDLGPGAGRLGGEVVAAG
                     PPADVERNPRSLTGLYLSGERSIPLPARRRAPDPARRLIVRGAAHHNLRNLDVAFPLG
                     LFVCVTGVSGSGKSTLVNDILYRRLANLLYGASEPAGAHAAIDGLEHLDKVIDVDQSP
                     IGRTPRSNPATYTGIYATIRDLFARTPEARVRGYKAGRFSFNVKGGRCEACRGDGLIK
                     IEMHFLPDVYVECEQCAGRRFNRETLEILYKGKSIADVLEMTCEEALSFFDAVPALRQ
                     RLRTINDVGLGYIHLGQPATTLSGGEAQRVKLSRELGKRATGRTLYILDEPTTGLHFE
                     DVRRLIDVLQRLVSEGNTVIVIEHNMDVIKSADWIIDLGPEGGDEGGLLVAEGPPEAV
                     ANIDASHTGAALREALGLRRQPRPAAGRARKAAASPRRRK"
     gene            complement(7714..8748)
                     /locus_tag="PLS90_01700"
     CDS             complement(7714..8748)
                     /locus_tag="PLS90_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPK01148.1"
                     /translation="MAGQFRFTFGPWNIHEGADPFGPEVRKTVRFNKKLEAYKQNGFD
                     GVQFHDDDAVPDLDALKPDQIERQARALRKTLDKHGLAAEFVAPRLWFDPRTIDGGYT
                     SNNAAQRRYAIDRSKQAIDIANVLGTDLIVLWLAREGTYIREAKDSKLAVERLVAAIN
                     EMLAHDPRIRIAVEPKPNEPMDIAFIPTTGHAVALAHMTDDPSRVGVVIESAHAILAG
                     LDPSDEMGFALANDKLWTVHLNDQNGLKFDQDKTFGSVDLRRALNQVRILDKHGYGRR
                     GEWVGLDVKAMRTQKDKVALKHLTNSRRVFLRLLEISRKLDDNKIAAMIGERDYEELE
                     MYILSALMGK"
     gene            8974..>9920
                     /locus_tag="PLS90_01705"
     CDS             8974..>9920
                     /locus_tag="PLS90_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HPK01149.1"
                     /translation="MSVPPFERYPLLMQPFYSPRPWGGTRLAETLHKDVPRENGPYGE
                     AWELSDHPDGRSTVANGPYAGRLFGDLVREYPRMMCGTERAPERFPLLVKYIDAAEDL
                     SIQVHPSDATAPAGERGKTECWYIMDCAPGAELIYGLQPGVDAERLREAVRANRVEQV
                     VRRIRIRPGDFVYLPPGTVHAVLGGTLLCEVQQASNATYRLWDWDRKPARALHIEESL
                     AVADYSGQPQLKPVAVESLEATREWVTLVRNDYFEVRTARWEAGELSENSLPNPHGLI
                     LNVVAGQGSLKVEGQPPQPLRLGQTWYLPAGLTEWALEAA"
     assembly_gap    9921..10020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10037..11140)
                     /locus_tag="PLS90_01710"
     CDS             complement(10037..11140)
                     /locus_tag="PLS90_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK01150.1"
                     /translation="MATAAHAAFSPVEITPDWEGFVRTIRRERVPDRVHFIELFIDPE
                     VQEAIARRYGLLDGLRRDDPHWDEQFQIRLQRFLGYDYVRATCEGLAMRLKVLNAEDT
                     SALKRAGGRQYVDEHKGPVTNWDEFEKFDWSRVEHITTRQLEYFEKHLPDDMCVVAFG
                     QIGHYAEMLNWLMGYETLCFALFNDRELVRAMFERLDHLYQAAVRTIVQFDRVKVIWG
                     SDDMGFRSGTLISPADLREFVLPGHKALARIAHDAGRPYILHSCGKLSPILPDLVDDV
                     RIDGKHSFEDTIGSVIEFKRKYGHKVAALGGIDVDFLCRSDEAAIRRRVRETLAACQS
                     EGGYCLGSGNSICNYIPVDHYLAMLDEGRKWRG"
     gene            complement(11450..13003)
                     /locus_tag="PLS90_01715"
     CDS             complement(11450..13003)
                     /locus_tag="PLS90_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPK01151.1"
                     /translation="MDTTLRDGEQTEGVSLLTEEKMTIAKLLLESVKVDRIEVASARV
                     SQGEHATLKAICDWARRARLLERIEILGFVDHKASVDWAHSAGCRVINLLTKGSRKHC
                     EMQLRRTLDQHLADIAQTVAYGRSRRMKFNVYLEDWSGGMLGCPDYVLAMLDQLVTLP
                     FERIMLPDTLGLLEPNQVTEFISAIVARYPGVHFDFHAHNDYGLATANSIAGLRAGAR
                     GIHTTINGLGERAGNSPLEEIVVAARDFTPFRCRIDERRLADASHLTQVFTGKRIAWN
                     KPIVGENVFTQTAGIHADGDRKGDLYHSRLTPDRFGRNRSYAMGKLMGKASLDYNLRK
                     LNIELSDDQKKLVLQRVVELADQKNVITSEDLPYIISDVLQSPEERLFEVLDFMVSTN
                     RGLRPVASVMIRYMGQTHQVSANGDGGYDAFMKALRSLESKVLGFRIPTLVDYTVHIP
                     PGGRTDALVETIITWEGGMKTRGVNPDQLVAAITATSNAINIIAQRMREKQPRPKPAR
                     ARSKAPVSG"
     gene            complement(13305..16073)
                     /locus_tag="PLS90_01720"
     CDS             complement(13305..16073)
                     /locus_tag="PLS90_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPK01152.1"
                     /translation="MPVAWEQIEPLLMRVGKPARYVGGEFNQIVKPRARVRACLAFPD
                     LYEIGMSYHGYRLLYERLNRQPGWAAERAFCPWPDYEALLREHGVPLYSLETRRSLAE
                     FHWVGFTLQHEVNFTNILTMLDLSGIPLESKDRTAAFPILIGGGEGAWSPEPISPFLD
                     AFVLGDGEEIVLEITALIERALAADWDRTRILRELARLPGVYVPEFYEFTYHADGTIA
                     GFTRRADAPDADHLPLRIHKRYFDVGGDLGSIKPVVPLLRTVHDRLVIEIRRGCVNGC
                     RFCQAGMVTRPVKERSLEQIVEIARQGIENTGYDEISLLSLSSADYTEIAPLVRRMTD
                     EFGPRGVSVSLPSLRINGFDVKLVDEIARVRKSGFTFAPEAGTTRLREVINKPVDQQR
                     FFEIIEDVFARGWRTVKFYFMIGLPTETQEDLDGIIEICEKAAELGERHHRRQARVNV
                     TLSPFVPKCNTPFQWEAQPPREELERRYQYVRGALRHGPFARRIDVKTSDTGGAFIEA
                     ALARGDRRMARAVRRAWELGARFDGWDECFRLGIWLRAFDEVGLDPTFYANRERPADE
                     ILPFDHLDSNVGRRFLWIDQRRARKQFVVEKCDTGKCAGCAVCNDVTDHTLSTARPDA
                     PPVAPPPVDGPDAPSAAEHDDLLRFNRAMEQVMAERDDEELLQSDAELEEEPPAEPCA
                     AMPGMGGRIPSAPAVQRLRFTYTRLGALRYLSHLDFQKVMGLVLRRAGAPLAYSHGFN
                     PQPKVQFAPPLSLGMGGRRELVDIQFREWLDPADLLARLNAVDLDGLEFLDVEEVPIA
                     APSLEMTLAGGAYRLELTAEPDAPRWADIEERLDAFAAAPEFVISVQKKKGLRQIDLR
                     QSVGSLTLETEPDGKRVFLLMMTFEAGSFVKPHEALGHVLGQPLELGVDVRVDRVELL
                     QRLPASHP"
     gene            16409..17593
                     /gene="mnmA"
                     /locus_tag="PLS90_01725"
     CDS             16409..17593
                     /gene="mnmA"
                     /locus_tag="PLS90_01725"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPK01153.1"
                     /translation="MNETLARLGDWSGRKVVVAMSGGVDSAVAAALLHRAGAEVIGVH
                     MRTWHYKGCEANDGLATCCSPADARDARRVADQFGFPFYAMDFQVNFRQAVIEPFIRD
                     YLDGRTPNPCVQCNNKLKLGSLLAKGGAYGAEAVATGHYGQIVTNERTGRHELRVGGD
                     GEKDQTYYLFGLTQPQLARMLMPVGHLSKPQVRALAEELGLHLHAKRDSVEICFVTDN
                     DYRRFLREEGAVDEARLAGAIVSTRGAVLGRHAGVHCFTIGQRKGLGLAAPRPLYVVD
                     LLPETQTVVVGYEEDLLAEGLEMDGVNWVAREPSREPFRALVKIRYRSPGYAATVEPD
                     PEDLSRARVLFDAPQRAVTPGQAAVAYDAPAGRTVLAGGWIRRRVMKEPAPGESGESG
                     AR"
     gene            17810..18865
                     /locus_tag="PLS90_01730"
     CDS             17810..18865
                     /locus_tag="PLS90_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01154.1"
                     /translation="MSKVFRVGTIGVGYTCNVHAPGWKESPHAELAALADLNEEALNR
                     VADRLEVKKRYTTARELIEDPEIDVVDICTPNMTHAPLAVAAFKAGKHVLCEKPLAPT
                     PAEIRRMIKARDAAGKLLMTAQHMRYLGTARAMKAEIERGVLGRIYHARAWILRRCGV
                     PLGPGFIRRELSGGGACIDIGVHILDLTLWLMGFPRPVTVSGFSRNELSKRPNAFSWG
                     RQPIPESMDVEDFAGGFVRFEGGQTLMIEISWLLHHDTPGEVQRVWLYGTEGGAEWPA
                     NKLIADDLPTRQLIDRQLILHDGGAPHALECIDFARALAQGLPSPVPAEESLYVQAIL
                     DGIYRSAEQGREVKVNL"
     gene            complement(18992..19963)
                     /gene="egtD"
                     /locus_tag="PLS90_01735"
     CDS             complement(18992..19963)
                     /gene="egtD"
                     /locus_tag="PLS90_01735"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HPK01155.1"
                     /translation="MSTALLYAETEPFEDGRLAFLRDVLAGLGQRPRRLDCKYFYDEE
                     GAGLFDAICELPEYYPTRTELAIMRENLDEIAECIGLDTTLIEYGSGNGRKTRLLLRQ
                     LDGRCTYVPLDIARQSLEAATRRLQADFPHLRIHPVCADFTQPCALPEPAFDAARRVV
                     FFPGSTIGNFHPAEAERFLRRVRQTCGRNGGLLIGVDLKKDPGLLVPAYNDARGATAR
                     FNLNLLARINRELGADFDLDRFTHYAFYNPQAGRIEMHLASLASQIVQVGGRSFRFNL
                     GDTIWTESSYKYTIDEFAAMALHAGLRLDQVWLDDDHLFSVHFYRGL"
     gene            complement(19963..21297)
                     /gene="egtB"
                     /locus_tag="PLS90_01740"
     CDS             complement(19963..21297)
                     /gene="egtB"
                     /locus_tag="PLS90_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HPK01156.1"
                     /translation="MMALSNPSPAALHSTALEREALALEYRRVRRFSEELARPLQVED
                     MVIQSMPDVSPTKWHLAHVSWFFETFVLAAAGAEYQPHHPQYGYLFNSYYEALGPRHC
                     RPRRGMLSRPTVAETFAYRRHVDDAMARLLESAGARRLGRIWPIILLGLNHEQQHQEL
                     MLTDVKHVLAQNPLRPAYLAGADSPAASAPPLAWFQFHGGLHSFGYSRPHDLDPLGFE
                     FAYDNEEPVHRRYLESFALASRPVTGGEFIDFIRDGGYNQPAFWLSDGWAAARENQWS
                     APLYWHEEEGEWRQFTLLGDRPVDPHEPVTHVSYYEADAFARWAGARLPTEFEWELAA
                     RPLPLDGNFVESGAFHPRSDGAALDEPSRRLHQMFGDVWEWTASPYAGYPGYQPAPGA
                     LGEYNGKFMCNQMVLRGGSCATAQSHIRPTYRNFFPPAARWQFSGLRLAKDA"
     gene            21825..22520
                     /locus_tag="PLS90_01745"
     CDS             21825..22520
                     /locus_tag="PLS90_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPK01157.1"
                     /translation="MPTPAELLQALESEITCCRKCPRLVAWREQVARVKTRRFADWEY
                     WGRPVPGFGDPAARLLIVGLAPAAHGGNRTGRIFTGDRSGDWLFRALHRAGFANQPTS
                     VHAGDGLRLTDCFIAASVRCAPPENKPTREEFANCHPFLAREMRLLGRVRVLVGLGRI
                     GFDEALRTAAETRGAVWKRKPAFGHGVVHQLAEGLWLVGSFHPSQQNTFTGKLTEPML
                     DGVFATARRLLAG"
     gene            22824..24509
                     /locus_tag="PLS90_01750"
     CDS             22824..24509
                     /locus_tag="PLS90_01750"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HPK01158.1"
                     /translation="MDRSAACIGRDMTRALLLCVLTCLPGWAPRLAAYGDAQNLIPEL
                     RLDQDAINAGEVPFETLFHTGMHFYTNRFTPADHYGEAPDGPRMSHQAFPGRPALRFL
                     RFNGLDAQTCLECHALAGMASRPEAGSPLMQFPRDPGVPGSAGLAANVFVLENFPAST
                     AGFIRNPPHNFGLGYVQRLAEEMSLALMSQRDAALAEAADAGATVTVALSAKGISFGT
                     LAAGPDGQLNLEGLEGVSEDLVIRPFQFKGIASTLRSFVANAMNFHFSVQARELLDRG
                     VIPNDNPNGTLPDDPRDELLEGDVTAVATFVAFVRPPVEMIEPQYALQAMQGRVVFEQ
                     VGCIECHVPELTIDDPNLTIEDPREQLKAYKALRQKGLAAEPPAVPALVNPEPDGSIL
                     PVVLLYEQRRAALAAGKSQPAGLPGYTRNLNLNDGPAETRPRLPHAEDGSIRVPLFSD
                     LKRHKMGADLADTLAQGTDSPGVAVPGDEFVTRPLWGVADTGPWLHDGRALTLTDAIL
                     MHKGPGSEANEVIENFENLEQDAKDLLHKFLLSLRTPPLEAPDGNAAEAWRGY"
     gene            25141..26229
                     /locus_tag="PLS90_01755"
     CDS             25141..26229
                     /locus_tag="PLS90_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01159.1"
                     /translation="MLRSANAKNGFGPYAACLALGAMMMLLAPAAWSQGGFVGLAPPD
                     AMDTVSGMSVGGGGAGNVALDRARGLQQPGMMGMPGPGGGPMPGPMFPGGMFPGDPGQ
                     AYQPPEALEPVIPTISVLVGTRVRDAVTKELLDEPVELKVPETEKGNFYDDGTHGDVM
                     ANDGKFAMVEFADDVLGEGSQRAKEQMVKALVVAEDLTPLEFYGFTIMGTDQRTPQPR
                     EKAWQMIPDPKGGPGFVLAEVETERPVEMPNYREWRNARDAKVKNDWSARFLRNYRVN
                     PDDIRSEFYHLYIPHPPQPPEVRPPVQEGWMPFSDPQSLMRAQSGEFNTGTTGGGYGT
                     PGITGGYPLGQGSGVTGQPIGNASSRYF"
     gene            26394..26855
                     /locus_tag="PLS90_01760"
     CDS             26394..26855
                     /locus_tag="PLS90_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01160.1"
                     /translation="MALNPQDVDEMHETTKKLLELWMRTKLVFLKAFSGEPITQEHEN
                     AYLQLKSEISRLYRVISDKLTPGLMFDGDKMLEMLKNAVTMEHLQRQSPAERSNLISA
                     WHRIYIRMTRTLGALEVMQSGYYPHLHRALLTGKGAGKGKGRWGRSAKKAA"
     gene            27100..30303
                     /locus_tag="PLS90_01765"
     CDS             27100..30303
                     /locus_tag="PLS90_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01161.1"
                     /translation="MKSLNSLRKGLGLAVLGMLLVTLAGCNKPLASFRLRGAEKRVQE
                     AEEKQAQQHTAELLQQTRNAINTTQNQLNQGDAVAAKESSAEAARLSKELLQRTTEAH
                     AIFLRDQANIWIDRARTNQAQQENAELFAQIQENNVEGTEAFGKQKYDKAIQIFGKVV
                     DDVQYLLSALRKKATDGLAEAESLKEELIAEGAPEHAPEFINKIDQQITQIRDNIERE
                     YNYRTALAIRDQARQTKQEGIQQTKKVKSDKQLTEIENLLDEATTLGAETYTYNLFSA
                     ITKEFENLVSQFYEENYDTVLTQAPKLKPQVEELILETKRVAAETKIKEVEGAINSLV
                     AMEARGYLPGRVEQLEALLADAREQYEQEAYVESREISDRALEEEQNILQEFDDLAQQ
                     HITTASDELATAEGVYEKMEHIFLRQIPGPWEGDALALENAKQALKEELRRRVNNARV
                     NLGMAQLQREEKDFDRAIEIARDVASEAEDVRQQTFRVVAHNAILDLSNMLSQYEGQG
                     GRQYAASEMDKAVEMLEQSKQLLATEQYREAVRRTADTKAQIEVLVQELERVAVNRIE
                     SAQQALAQAKADRAEEYEPIAFTQALVELQAAQEALAAEGRHIAIEAAIQAENLAAEA
                     STNALRQWVQELMAEADTLINNAREAEADRYAPEKLDRAFAIRRNLQTLYDQGQYREA
                     VDVGAQTVQQAHEALYAKVIEAENAIAKAKRFEGWEFENARLADAMVSAKYAREMMAE
                     GRFRLAEQHAWNALVKAEEAAVNARRDGFETRMASLAARVEDAQRKGAGYYQTQDLAS
                     LLAEMNRLRMEFDPHDYEDYAQQVDLVEAKLIALMELTPDVLKALVLDMSQRMTELEQ
                     RGAALYMPNKLAEVERKLKYAQIDYQAGKYRPSYQNAKDAWAVLDEIEQNLEEREFDA
                     ALNDLMVELSDQIRAFAPVLDMGANTLLELVIGPQGQARATSILGVRSPTDLRDSITE
                     IGARIRRMQAPRSRETLQQEMLRMMEIAKTAASNFEKMLIMDQYSRDQAREIVQTAFL
                     QMYQARARQQELQRMIEYPNPQFKPRGVERVVSFQDY"
     gene            30520..31092
                     /locus_tag="PLS90_01770"
     CDS             30520..31092
                     /locus_tag="PLS90_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01162.1"
                     /translation="MALSKPAPPRAAGKQKKADVRFKLTKAQKAYVEQLVTLDNRIAV
                     CREYIALWMNFFRFFAEDMTRKEITAAEEKQFFQVMTALTRKHFTFVELMAETFDRGN
                     DIIAVLSTAVSLTHIQNMPENTRGKLELDWHNLFLDMNRTLGRLLRRLPGNMTLSEAL
                     ASVNAKPVAPDPGAARADGKGAKPAAAAGA"
     gene            31160..31693
                     /locus_tag="PLS90_01775"
     CDS             31160..31693
                     /locus_tag="PLS90_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HPK01163.1"
                     /translation="MTLWHDTGARRFGSRDEIEAAVARGESLAGACLEGLDLAGAKLA
                     GADLTGARLSGANLTGAVLADATLTRARLDEVVAPEADFSRIHAEFAVFGLARLAGAR
                     FHAADLRRSNFVGAVAPGADFSQADVYYARFAGADLSQACFTQANVQRAVIRAADIRH
                     ARFEGARGKATFDRNTQ"
     gene            31755..32345
                     /locus_tag="PLS90_01780"
     CDS             31755..32345
                     /locus_tag="PLS90_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK01164.1"
                     /translation="MLLRHFMLDVGPQEANCYLLADLAAKTGILVDAGAFEPAIVALA
                     AEHDLRMAAILLTHLHWDHVDGLPEYLRHWPAARVIAPAALATAEVERTAPGEELEIE
                     PFRFRVLRTSGHTPESVSYYCAEHAICFVGDALFAGSIGGTADEEKFREETGCIRDHL
                     FTLPDATELLPGHGPVTTVAIERAANPFLKPGFGRN"
     gene            32525..34141
                     /locus_tag="PLS90_01785"
     CDS             32525..34141
                     /locus_tag="PLS90_01785"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK01165.1"
                     /translation="MRPAIQDLPTDRPLHRLGSWIALGVWLALLATILIDRMAQPWRP
                     LLAWCGIALLALVDCPSPRREGGALRRRLAVAGLGALLSTILAIDIGMLVDRAPQAIN
                     VWYLRARHELWHDFWTDPSPLLILATWWFAAPFVIDAWRARREPRARTAEALTRRHAL
                     WLLAWAFWIGVAMFASIRPDRAYYRFPQEMGLHAPLFLFWIRRAGAAPDLQRRLARIA
                     PWMMGAALLAGLVVAIAYVTGDWNRQVAIGRAEIWRTELVERQYINGWQWRLNFPLGH
                     HNRMGFFSMLTALLLLMAARRRGGWMRWVAVAAMLVALATLGFSLNRGSFLALVAGLT
                     VMTVLWLGRRAAWLLLLLPAAWLAMSDLQRQQVLSLVDRETYRSPQSTVVTRLDHWRL
                     AARLIRENPVFGIGYSWRHFEEYAEDYAKQVGAPVYEKAHAHNYWLQTAAETGLPGML
                     LYLGWTIGRWSLLLALVRRRRRLPAGERGLIEGWCGVEAAIQVYCLGNLPLRLGLGLL
                     TWGIWAVMWVDLVRLWRAAQDPAGVPSETS"
     gene            34224..34697
                     /gene="smpB"
                     /locus_tag="PLS90_01790"
     CDS             34224..34697
                     /gene="smpB"
                     /locus_tag="PLS90_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPK01166.1"
                     /translation="MARNEADGVKLVCQNRKARHEYHIEETWEAGLELRGTEVKSLRQ
                     GKGSLQEAYAQVKGGEAWLLQFHIPPYEQGNRFNVDPVRPRKLLLHRREIGKLAGAVS
                     RQGYTLVPLRVYFRRGRAKVEIAVAKGKKLYDKREAMKERDTRRDLDRALRDRRV"
     gene            34831..>35652
                     /locus_tag="PLS90_01795"
     CDS             34831..>35652
                     /locus_tag="PLS90_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01167.1"
                     /translation="MQNPPSSTPPPASVSLFRLPHWVGYGLFVAVALALFFTAVHSYR
                     QFVDDYQYNRPRYAAERALDLLAQNRTDEAAIEVQVLMRDRPIYTGLLNQEMSEVYEG
                     PVFRNRVTTFERLTAALLDHDLLLEAEMVGWKALLEFHIASRALEMFVPWELMHAVKV
                     LRDDWSSAFQISKLLAAHGVKNKVRLPNEIRPNPFPVDPAPFADLNRQMPDPLIQGLF
                     DYYQSRLAPRPPEARNLRNRAAAQLANAEKATVIPGVQRQLQAFRHRILLEGDRRQ"
BASE COUNT         5928 a        10647 c        13341 g         5636 t
ORIGIN      
        1 cggccgtcaa gaaagaggcc gacgcagccc tcaaggcgca cggcctgatc aagaagaagg
       61 tccggttcga agacgaggcg gtcgacacga tcatccagcg ctatacgcgc gaggcgggcg
      121 tgcgcaatct ggaacgcgag atcgcgacgt tgtgccgcaa gacggcgcgg cggttgatcg
      181 cggggggcga agagagcgcg ccgcgcatca cgccgcaact ggtccgcgcg ctgctcggac
      241 ccgaacgtca ccacgatctg cagattcatg acaagacgga ggtcggcgtc gccaccgggc
      301 tggcctggac cgatgccggc ggcgagatcc tgccgaccga aacgacgacg atgcccggca
      361 aggggaacct gcaactgacc gggaagctgg gcgaggtgat gaaggagtcg gggcaggccg
      421 cgttgagcta tctgcggtcg cggtcggggc aatacggcct cgcgcccgat ttccacaagg
      481 agctcgatat tcacgtccac atccccgagg gggcgattcc caaggatggt ccgtcggcgg
      541 gggtgacgct cgtcacgtcg atgtactcgg cgctgaccgg gcggcccgtg cgccaggatg
      601 tggcgatgac gggcgagatc acgctgcggg gcaaggtgct taagatcggc gggctgaagg
      661 agaaggttat cgcggcgcac cgcaaccatc tgcgcacggt gctgattccg gcggacaacg
      721 agccggagct ggaggaaatc gcgccggaga ttctgcggga gctcgatatc cggccggtgc
      781 ggacggtgga tgaggtgctg gcgctggcct tggccgacga gccggcgcgt cccgacaaga
      841 gcggcggcaa gccccggcgg gggcagtccg gccggggggc gggacggcgc caaccggcga
      901 gggggctcgg gatcaacgcc tgacgcggcc gggggcctgc ctgatacaag gacaacctag
      961 tcatgccgcc gagcggcggg cttcaggagg ccagggcgcc gtcggtgaag ccttcgagcg
     1021 agcggtcctt caaggtatcg aggtaattgc gcgcgaggcg cagggcgtcg gcgtaggaga
     1081 cgtaattgcg cgccagttcg tcgaagatgc gttcgccgaa ccgggcggcg atcccgtagg
     1141 accggcgccc ctcggcgaca taccaggcaa agacatcgcc cgacatgatg aggctgccga
     1201 cgatgtggtc ggtgctgtcg ccgcggatct ggccgagcag ctcggggaac aggccggtca
     1261 tgaacagcgt gtagtcaccg atgtatttgt ggcggtcgcg ctcggggatg gcccgcgcct
     1321 gctcggattc gcgcaacgcg acgagcatgt cggcgatcgc ttcgacgggg cggccgtcgg
     1381 cgcgccggaa gcggtggatc cggtcgacat ggatgaactc gaccatcacg ttttcctcga
     1441 ggtagcgggc catgtcgctg tcgcggatgc cggcgtagtc gaagccgaag cccagggccc
     1501 ggcggaagac gacgcgaagc gggtgattgg agcggatcat ctggcatctc ccgcgtaaag
     1561 gaaatctgtt gagtatcagt atagtgcaag cgggcggggg gcgaagacga aaaatgcgag
     1621 ggggtggagg aggaagcggc aattcggcgg gtgggacgag agggcgggtt ttcgagagcc
     1681 gaggacgagg gacgagagcc gggagccgaa tcttgcgggg gctttggggg cagggtggag
     1741 atgctggaca atccgcggcg ggcgcggcaa gaatggcggt tccgcgcgcg gcggggggcg
     1801 gtccgagagc cgaccggacc ggcgggggcg ccggtcccac aggggcggca gcagacaggg
     1861 gaaaggcgaa ggaacgatga tcaagccgct ggtgaccgga ctgatcgggt tcggcaatat
     1921 cggatcgggg gtgctgcggg cgatggcgga gcgggccgag gagctcaagg cgcgcctgcc
     1981 ccggccgatc gtgctcaagt cgatcgccga caaggacacc acgacgcggc gcgacgcccc
     2041 gtatcacaag gaccagcttc acggcgacgc ggcggtggtg ctggaggacc cggagatcga
     2101 ggcggtgatg gagctgatcg gcggcgtcga accggcgcgg acgttcgtcg aacgggcgtt
     2161 gcgcgcgggc aagcatgtcg tcaccgcgaa caaggcgctg ctggcgacgc acggcccgga
     2221 gctgctggcg ctggcggcgg aaaacggggt cggcctgctg tatgaagcgg ccgtcggcgg
     2281 cggcatcccg atcatccgca cgctgcagca ggggttgtcg gccaatcggc tgacggcggt
     2341 gcggggcatt ctgaacggca cctgcaatta catcctgacg cggatggcgg aggcggggct
     2401 gccgtttgac caggcgctga ccgaggcgca ggaattcggc tatgccgaac cggacccgac
     2461 gtacgacatc gagggctacg acacggcgca caagatcgcg attctggcct cgctggcatt
     2521 cgggatggac atccggttcg acgatgtgtt tgtgcacggc ataacggaca tccatccggt
     2581 ggacatcaag catgcgcgcg aactgggcta cgcgatcaag ctgctgggga tcgcgcagcg
     2641 ccacgcgagc gacgggtcgg tgatcgtgcg cgtgcacccg acgctggtgc cgctggcgtc
     2701 gccgctgggg atggtggggg gggtgttcaa cagcatcctg gtggaaggga cgccgatcgg
     2761 gccgacggtg tattacgggc ggggagcggg gcccgaggcg acgagttcgg cgatcctgag
     2821 cgacctgatg gcgctggcgt cggacacgca cgggttcaac gccgcgcgcg acgcgcggtt
     2881 gacggtgcgg gtcggcgagc ggacgctgcg gccgatcgac cagttgcgga ccaattatta
     2941 tatccggttc agcatggtgg atcgtcccgg agcgatggcg gagatcgggc gcgcgctggc
     3001 cgagcacaat gtctcgatcg agtcgatgat ccagcaccgg gtggagctgg cgcaggggat
     3061 ggccacgatc acgatcgtga cgcacgaggc gctggagaag gacgttcagg gagcgatcgc
     3121 ggcggtcgag gcgagcgaga agtcggcggg caaggcgttc gtcctgcgcg tcgaggagta
     3181 gcgcgtcgag cgggcggtcc gcggccgacg cgaaatcgaa gcggcggacg ccgcccatcc
     3241 ccggcaccgg cgcggtccga tgcgcatcac gctgctggcc aatcccgaca atctgcacgt
     3301 ccagcgctgg atcgaatacc tcgccggccg cggccacgcg ctgacgctga tcaccgatcc
     3361 gttcacgcgc gcgcgcccgg ccgcgtgcga gatccacgtc atccgctgga atccgttgac
     3421 caagctgctg gcgttccggc tgacgccgcg cccgctgggg aacgaccgct ggaagcatct
     3481 gcacgcgcgg ccgctcgtgc ggcggtcgcg tcccgacgtc gttcacggtt tcgaggcgtt
     3541 caactggggt ctggccacgg cgctggcggg gccgtatccg cgcgtactga cgccgtgggg
     3601 gaatgatgtg tttgagattc cggggcgctg gccgctggcg cggcggatga tcgggcgggc
     3661 gctgcggagc gtcgacgcaa tctcgaccaa cgacgagacg ctcgcgccgt atctggccag
     3721 ggagttcggc gttgatcccg cgcgcgtgca cgggttcacg tggggggtcg acccggggct
     3781 gttccacgcc gagctgccga ccgaagcggc cgactggcgg cggcggctgg agattcccgc
     3841 cggcgcgccg gtgatcctga gcccgcgcaa cttccacccc tactggggga gcgaacagat
     3901 ttgcggggcg attccgcggg tgctgcgcca ggtccccgcg gccgtgttcg tgatcctgaa
     3961 ggggttgggg ggcgacgcgg cggcgttcgc ggaggcgcgc cggcggctcg ccgccgaggg
     4021 tcatggcgcc gcgctgcgct ggctggccga gccgctggcg ccgcgcgaga tggccgccct
     4081 gttcaacctg gccgacgtgt tcatctcggt gccgaagacc gacctgctgg cgcagacgat
     4141 tctggaaggg atggcgtgcg gctgtttccc ggtgctgggc gaccggccgg cgtatcgcaa
     4201 gcacctgcgg ccgggcgaga acgggctgct ggtggaggac gagacgccgc aggcgctggc
     4261 ggcggcgctg ctcgtggcgc tggcgaacga tacgctgcgg gccaacgcgc gccacgagaa
     4321 cccccggctg atcgcggccg tagagaactg gcagctcaac gcgcagcgga tggaggcagt
     4381 ataccagacg gcgatcgagc ggttcggccg gtcggccggg tgattacttg cgccggcggg
     4441 gtgaagcggc cgccttgcgg gcgcggccgg cggccggccg gggctggcgg cgcaatccca
     4501 gggcttcgcg cagcgcggcg ccggtgtggc ttgcgtcgat attggccacc gcctccggcg
     4561 ggccttcggc gaccagcagc ccgccttcat cgcccccttc cgggccgagg tcgatgatcc
     4621 aatcggcgga cttgatcacg tccatgttgt gttcgatgac gatgacggtg ttgccctcgc
     4681 tgacgaggcg ctggaggacg tcgatcagac ggcggacgtc ctcgaagtgc aggccggtcg
     4741 tcggttcatc caggatgtaa agcgtgcggc cggtggcgcg cttgccgagt tcgcggctca
     4801 gcttgacgcg ctgggcctcg ccgcccgaga gggttgtggc gggctggcca aggtggatgt
     4861 agccgaggcc gacgtcgttg atcgtgcgca ggcgctggcg cagggcgggg acggcgtcga
     4921 agaacgagag cgcctcctcg caggtcattt ccaggacgtc ggcgatcgat ttccccttgt
     4981 agaggatctc cagggtttcg cggttgaaac ggcggccggc gcactgctcg cactcgacgt
     5041 agacatcggg caggaagtgc atctcaatct tgatcagccc gtcgccgcgg caggcttcgc
     5101 agcgcccgcc cttcacgttg aatgagaacc ggccggcctt gtagccgcgg acgcgggcct
     5161 cgggcgtgcg cgcgaacagg tcgcggatcg tggcgtagat gccggtatag gtggcggggt
     5221 tggaacgcgg ggtgcggccg atgggcgact gatcgacgtc gatgaccttg tccagatgct
     5281 cgaggccgtc gatggcggca tgggcgccgg ccggctcgga ggcgccgtag aggagattcg
     5341 ccagccgccg atagaggatg tcgttgacca gcgtggactt gccggaaccg gagacgcccg
     5401 tcacgcagac gaacaatccc agggggaagg cgacgtcgag attcctgagg ttatggtgcg
     5461 cggcgccgcg gacgatcaag cggcgcgccg gatcgggcgc gcggcggcgc gcggggagcg
     5521 gaatcgaccg ttcgccggag agatacaggc cggtcagcga gcgcgggttg cgttcgacgt
     5581 cggcgggcgg tccggcagcg acgacttcac cgcccagccg gccggcgccc gggcccagat
     5641 cgatcacata atcggcttcg cggatcgtcg tctcgtcgtg ctccacgacg acgagggtgt
     5701 tgcccaggtc gcgcagtttc ttgagcatgt cgagcaggcg gccgttgtcg cgatggtgca
     5761 ggccgatgga gggctcgtcg aggatgtaga ggacgccgac cagttgcgag ccgatctggg
     5821 tggcgaggcg gatgcgctgg ccctcgccgc cggagagggt gccggcggcg cggtcgagcg
     5881 agaggtaatc caggccgacg ttgaccatga actggaggcg gtcgcggacc tccttgatgg
     5941 cctggccgcc gatcgccagt tcgcgtttgg accagcgcgc gccgtcgagg aaggcgaggg
     6001 cttcgcggac ggtctggccg cagaactcgc tgatgttgcg acccttaatg gtgacggcca
     6061 ggctgccggg cttcaagcgc gcgccggcgc aggccgggca gggctggtcg ctgtagtagc
     6121 cgcgcaggtg ctcgaggata tcctcgctgg tggtttcgcg caggcggcgc tggatgcgcg
     6181 ggacgaggcc ctcgaacggc ttgttggttt cgaagcgcga gccggtgccc atgacgaacc
     6241 tgaccgggac cggctcctgg ccggtgccga acagaatcgc gtcgcgctgg cgctgggtca
     6301 ggctgcgcca cggcttgtcg gggttgatcc tgaggtgctt gagcagggcg aggacgtact
     6361 gcttgcccca gccctcgccg cgttcctcgt cgtcggcgcg ggccggggcg gcggggcggc
     6421 gaccgggttt ggccaccggt cgggggtcgt cgccgatgtc ggcctcggcc gcgcgaccgg
     6481 cggcgccgtt gcggaagtaa ccggcccacg gcagcacggc gccttcggcg attgagagcg
     6541 cctcgttcgg gacgatccgg cgcgggtcga cctcgggcag cgtgcccagg ccgttgcagc
     6601 gcgggcaggc gccgaacggg ctgttgaacg aaaacatgcg cggggagagc tcgacgatct
     6661 gcgggccgtg aaccggacag gccatgcgct cgctgaaggt gatctcctcg ccgggctgat
     6721 cgccgggtcc ggcgaggcgc tcgatgagcg cgaggccgtc ggccttcttc agcgcggttt
     6781 cgaccgagtc ggtcagccgc tggcggatct tcggcgtgac gacaaggcgg tcgatgacga
     6841 tcgagatgtc gtgcttgagg cgcttgttga gcgagatcgg ctcgtcaacc aggtggattt
     6901 cggcgtcgat cttgacgcgc tggaagccct cgcgcgcggc ctgctcgaag agctgcttgt
     6961 attcgccctt gcggccgcgc acgacggggg ccataacgag aattttcgtc ccctcgggcc
     7021 aatccaggat cttgtcgacg atctcctgga cggtctgctg ctggagcggc cggccgcaga
     7081 ccggacagtg cgggctgccg atgttggcat agaggacgcg caggtaatcg tagatttcgg
     7141 tgacggtgcc gacggtcgag cggggattgc ggctgacggt cttctgctcg atcgaaatcg
     7201 ccgggctgag cccctcaata tgctcgacgt gcggtttgtg catctggtcg aggaactggc
     7261 gggcgtagga ggagagcgac tcgacgtagc ggcgctggcc ttcggcgtaa atcgtgtcga
     7321 aggcgagcga gctcttgccc gagccggaca ggccggtcac gacggtcagc gtctggcgcg
     7381 ggatggtgac gttgatgtgt ttgaggttat gctccgcggc gccggtgacg acgatgttgc
     7441 gaaggcgttc gggtttggac atggggacgg gctgattcgc tggcggaata gcgggcgcga
     7501 cgatgccccc gcgcgcgtgg ggcgaaggag aaacatttta gcggggcgga aggaaaagcg
     7561 caagcgcaag cgaagaaata gcgaaaacga aggggagggg ctgacggacg gaagggacat
     7621 cagggacgca aaggacacaa gaaataaaga ctgggtccct gaatcggccg gagatgtcct
     7681 ctccgtcctt cagcagaatg gcgtccagac gttttacttg cccatcaggg cgctaaggat
     7741 gtacatctcc agctcttcgt aatcgcgttc gccgatcatg gcggcgatct tgttgtcgtc
     7801 cagcttgcgc gagatctcca gcaggcgcag gaagacccgg cggctgttgg tcaggtgttt
     7861 cagggcgacc ttgtccttct gggtgcgcat cgctttgacg tccaggccga cccactcgcc
     7921 gcgccggccg tagccgtgct tgtcgagaat ccggacctgg ttgagcgcgc ggcgcagatc
     7981 gaccgagccg aaggtcttgt cctggtcgaa cttcaggccg ttctgatcgt tcaggtggac
     8041 ggtccacagc ttgtcgttgg cgagggcgaa gcccatttcg tcggagggat cgaggccggc
     8101 gaggatggca tgggcgcttt cgatgacgac gccgacgcgg ctcggatcgt cggtcatgtg
     8161 cgcgagggcg acggcatggc ccgtcgtggg gatgaaggcg atatccatcg gctcgttggg
     8221 tttgggctcg acggcgatgc ggatcctggg gtcgtgggcc agcatctcgt tgatcgcggc
     8281 gacgaggcgt tcgacggcga gcttgctgtc cttggcctcg cggatgtagg ttccctcgcg
     8341 ggcgagccag aggacgatca ggtcggtgcc gagaacgttg gcgatgtcga tcgcctgctt
     8401 gctgcggtcg atggcgtagc gccgctgggc ggcgttgttg gaggtgtagc cgccgtcgat
     8461 cgtgcgcgga tcaaaccaca ggcggggggc gacgaattcg gcggccaggc cgtgcttgtc
     8521 gagcgtcttc cggagcgccc gcgcctggcg ttcgatctga tcgggcttga gcgcgtccag
     8581 atcggggacg gcgtcgtcgt catggaactg gacgccgtcg aagccgttct gcttgtaggc
     8641 ttccagtttc ttgttgaagc gcaccgtctt gcggacctcg gggccgaacg ggtcggcgcc
     8701 ttcgtggatg ttccagggtc caaaggtaaa acggaattga ccggccatgg tatcatctcc
     8761 cctcgagtct gccgcggctg aaggcgcggt gagaaatcgt tatcctaaag gaaaaatcgg
     8821 gcgcgcgcca gatcggatcg tcgttgaaac cgggatcggg attgccggtc gaacggccgg
     8881 gcgttcgggc ttcacatcgc cgggcggttt ccgataggct gaggagtggc cccccgcccg
     8941 gccgccgcag cccgccgcga gagaatccgc ccgatgagcg tcccgccgtt cgaacgctac
     9001 ccgctgctga tgcagccgtt ttactcgccg cggccgtggg gggggacgcg gctggccgaa
     9061 acgctgcaca aggacgtgcc gcgggaaaac ggcccctacg gcgaggcgtg ggagctgtcg
     9121 gatcaccccg acggccgctc gacggtcgcg aacggcccct acgccggccg gttgttcggc
     9181 gatctggtcc gcgaataccc gcggatgatg tgcggcacgg agcgggcgcc cgaacgcttt
     9241 ccgctgctgg tcaagtatat cgacgccgcc gaggatttgt cgatccaggt ccatccctcc
     9301 gacgcgacgg cgccggcggg cgagcggggc aaaaccgaat gctggtacat catggactgc
     9361 gcgccgggcg cggagctgat ttacggcctg cagccggggg tcgacgcgga gcgcctgcgc
     9421 gaggccgtcc gcgccaatcg ggttgagcag gtcgtgcggc gcatccggat ccggccgggc
     9481 gacttcgttt atctgccgcc cggcacggtc catgccgtgc tgggggggac gctgctgtgc
     9541 gaggtgcagc aggcatccaa cgccacgtat cggctgtggg actgggatcg caagccggcc
     9601 cgggcgctgc acattgagga gagcctggcc gtcgccgact acagcggcca gccccagttg
     9661 aaacccgtgg cggtggaaag tctggaagcg acgcgcgagt gggtgacgct ggtccggaac
     9721 gattacttcg aggtgcgcac cgcgcgctgg gaggcgggcg agctatcgga gaatagtctg
     9781 cccaaccctc atgggctgat tctgaacgtg gtggcgggcc agggcagcct caaggtcgaa
     9841 ggtcagccgc cgcagccgct gcggctgggg cagacctggt atctgccggc cgggctgacc
     9901 gagtgggcgc tggaggcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 ttgcgatccc gatcgttcag ccgcgccatt tgcggccttc gtcgagcatc gccaggtagt
    10081 ggtcgaccgg gatgtagttg cagatcgagt tgcctgagcc gaggcagtag ccgccctcgg
    10141 actggcaggc ggcgagggtc tcgcgcacgc ggcggcggat cgccgcttcg tcgctccggc
    10201 agaggaagtc gacgtcgatc ccgcccagcg cggcgacctt gtggccgtat tttcgcttga
    10261 attcgatgac gctgccgatg gtgtcctcga aggaatgctt gccgtcgatc cggacgtcgt
    10321 ccacgaggtc gggcaggatg gggctgagct tgccgcagga gtgcaggatg taggggcggc
    10381 cggcgtcgtg ggcgatgcgc gccagcgcct tgtggccggg caggacgaat tcgcgcaggt
    10441 cggcggggct gatcagcgtg ccggagcgga agcccatgtc gtcgctcccc cagatgacct
    10501 tgacgcggtc gaactggacg atggtgcgca cggccgcctg atagagatga tcgagtcgtt
    10561 cgaacatcgc ccgcaccagc tcgcggtcgt tgaacagggc gaagcagagc gtttcgtagc
    10621 ccatcagcca gttgagcatc tcggcgtagt ggccgatctg gccgaaggcg acgacgcaca
    10681 tgtcgtcggg caggtgtttt tcgaagtact cgagctggcg ggtggtgatg tgctccacgc
    10741 gggaccagtc gaacttttcg aactcgtccc agttggtgac cgggcccttg tgttcatcga
    10801 cgtactggcg gccgccggcg cgcttcagag cgctggtgtc ctcggcgttg aggaccttga
    10861 ggcgcatggc caggccctcg caggtggcgc ggacgtagtc gtagccgagg aagcgctgca
    10921 ggcggatctg aaactgctcg tcccagtggg ggtcgtcgcg gcggaggcca tccagcaggc
    10981 cgtagcgccg ggcgatggcc tcctgaacct cggggtcgat aaacagctcg atgaagtgga
    11041 cgcgatcggg gacgcgttcg cggcggatcg tgcggacaaa gccttcccag tcgggggtga
    11101 tctcgacggg ggagaaggcg gcatgcgcgg cggtcgccat gattggcctc gggtgagagc
    11161 aaggggatgg aagcgtcccg gaggggaaga cgcgcgcgac ggattcggaa tcaggatatg
    11221 ctcatcctta ttttccgcaa gggaaaagtg gaacggatcg ggactgccct tgatccttca
    11281 cgggtcggaa agcggagtag ataaagcggg accgagggcg tccggcccgc gttgcgccgg
    11341 acggggccgg gggggaacca cgcgcggggc cggggccgcc gctgtcgacc ctacccctac
    11401 ccctgaccga gcgaatggcc aaactccagg ggcccgccgg tggcggccct caaccggaga
    11461 cgggggcctt gctccgggcg cgcgcgggtt tgggacgcgg ctgcttctcg cgcatgcgct
    11521 gggcgatgat gttgatcgcg ttggaggtgg cggtgatcgc cgcgacgagc tggtcgggat
    11581 tgacgccgcg agtcttcatc ccgccttccc aggtaatgat cgtctcgacg agggcatcgg
    11641 tccggccccc cggggggatg tggacggtgt agtcgacgag cgttggtatc cggaagccga
    11701 ggaccttgct ctcgagcgag cgcagggcct tcatgaaggc atcgtagccg ccgtcgccgt
    11761 tggcgctgac ctgatgggtc tggcccatgt agcggatcat gacgctggcg accgggcgca
    11821 gcccgcgatt ggtcgagacc atgaagtcca ggacctcgaa cagccgttcc tcgggcgact
    11881 gcagaacgtc ggagatgatg tagggcaggt cctcgctcgt gatgacgttc ttctggtcgg
    11941 ccagttcgac cacgcgctgc aagaccagtt ttttctgatc gtcgctcagc tcaatgttca
    12001 gtttgcgcag attgtagtcc aggctggcct tgcccatcag cttgcccatc gcgtagctgc
    12061 ggttgcggcc gaagcggtcg ggggtgaggc ggctgtggta gaggtcgccc ttgcggtcgc
    12121 cgtcggcgtg gatgccggcg gtctgggtga agacgttctc gccgacgatc ggcttgttcc
    12181 aggcgatccg tttgccggtg aagacttggg tcaggtggct ggcgtcggcg aggcggcgtt
    12241 catcgatgcg gcagcggaag ggagtgaagt cgcgcgcggc gacgacgatt tcctccagcg
    12301 gggaattgcc cgcccgttcg cccagcccgt tgatcgtcgt atggatgccg cgcgcgccgg
    12361 cgcgcaggcc cgcgatcgag ttggccgtgg ccaggccata gtcgttgtgg gcgtgaaaat
    12421 cgaaatgaac gccggggtag cgcgcgacga tcgccgagat gaactcggtc acctggttcg
    12481 gctcaagcag gccgagcgta tcgggcagca tgatccgctc gaacgggagc gtcacgagct
    12541 gatcgagcat cgccaggacg tagtccgggc agccgagcat gccgccggac cagtcctcca
    12601 ggtagacatt gaatttcatt cggcggctgc ggccgtaggc gacggtctgg gcgatatcgg
    12661 ccagatgctg gtcgagcgtg cggcgcaact gcatttcgca gtgcttgcgc gagcccttgg
    12721 tgagcaggtt gatgacgcgg cagccggccg aatgggccca gtcgaccgag gccttgtgat
    12781 cgacgaagcc gaggatctcg atgcgctcga gcaggcgggc gcgccgggcc cagtcgcaga
    12841 tcgccttgag cgtggcgtgt tcgccctggc tgacgcgcgc cgacgcgacc tcgatgcgat
    12901 cgaccttgac cgattcgagg agcagtttgg cgatcgtcat cttttcctcg gtcaggagcg
    12961 aaacgccttc ggtctgctcg ccatcgcgca gcgtcgtatc catgatttcg aacgtcggcg
    13021 tgcgcgcagg ccgcggtgca ggctgctttt ttttcctggg tttcgtggtg gtgggcacgg
    13081 tccggatctc ccccgagtgg cttccggcgg cgcatccccc gcggtgcaac ccatccggaa
    13141 gggtagaatc ccaacattat ccgcgcgcgg ggggatcgat gcaacaggca acgcgggggg
    13201 aagaggggcg gttcgaggta cgagggacga ggacgagagc cgagagccga gagccgaaac
    13261 ctggtgagcc gttgggggcg agatacgagg acgagggacg ggattcaggg atgggaggcg
    13321 gggaggcgct ggagtaattc gacgcggtcg acgcggacgt cgacgcccag ctccagcggc
    13381 tggccaagga catggcccag cgcctcgtgc ggtttgacga aacttccggc ctcgaacgtc
    13441 atcatcagca ggaacacgcg cttgccgtcg ggttcggtct ccagcgtaag cgagccgacc
    13501 gactggcgca ggtcgatctg gcgcaggccc ttctttttct gaaccgaaat gacgaactcg
    13561 ggcgcggcgg caaaggcatc caggcgctct tcgatatccg cccagcgcgg cgcgtcgggt
    13621 tccgccgtca gttcgaggcg gtaggcgccg ccggcgagcg tcatctcgag ggagggcgcg
    13681 gcgatcggga cctcctcgac gtccaggaat tccagtccgt ccaggtcgac ggcgttgagg
    13741 cgcgcaagca gatcggccgg atcaagccat tcgcggaact ggatgtcgac cagttcgcgg
    13801 cgtccgccca tgccgagcga gagcgggggg gcgaactgca ccttgggctg cgggttgaag
    13861 ccgtgggagt aggccagcgg ggcgccggcc cgccgcagga ccaggcccat caccttctgg
    13921 aaatccaggt gggagaggta gcgcagggcg cccagccggg tgtaggtgaa gcgcaggcgc
    13981 tggacggcgg gggccgacgg gatgcggccg cccatgcccg gcatggccgc gcagggctcg
    14041 gcgggcggtt cctcctcgag ctccgcatcg gactggagca attcctcatc atcgcgctcg
    14101 gccatcacct gttccatggc gcggttgaag cgcagcaggt cgtcgtgctc ggcggcggac
    14161 ggcgcgtccg ggccgtcgac cggcggcggg gcgacgggcg gggcgtcggg cctggcggtc
    14221 gagagcgtgt ggtcggtgac gtcgttgcag acggcgcagc ccgcgcactt gccggtgtcg
    14281 catttttcga cgacgaactg cttgcgggcg cggcgctgat cgatccagag gaagcggcgg
    14341 ccgacgttgg aatcgaggtg atcgaacggc aggatttcgt cggcgggccg ctcgcgattg
    14401 gcgtagaagg ttgggtcgag gccgacctca tcgaacgcgc gcagccagat ccccagccgg
    14461 aagcactcgt cccagccgtc gaagcgggcg cccagttccc aggcgcggcg gacggcgcgg
    14521 gccatccggc ggtcgccgcg cgcgagcgcc gcttcgatga atgcgccgcc ggtgtcggag
    14581 gtcttgacgt cgatgcgccg ggcgaacggc ccgtgccgca acgcgccgcg cacatactgg
    14641 tagcgccgct ccagttcctc gcgcgggggc tgggcctccc actggaaggg ggtgttgcac
    14701 ttggggacga acggcgaaag cgtcacgttg acgcgggcct ggcgccggtg gtggcgttcg
    14761 cccagttcgg ccgccttttc gcagatctcg atgatgccgt ccaggtcttc ctgcgtttcg
    14821 gtcggcaggc cgatcatgaa gtagaacttg accgtgcgcc agccgcgcgc gaagacgtcc
    14881 tcgatgattt cgaagaaccg ctgctgatcg accggcttgt tgatgacttc gcgcaggcgg
    14941 gtggtgcccg cctcgggggc gaaggtgaag cccgacttgc ggacgcgggc gatctcgtcg
    15001 accagcttga cgtcgaagcc gttgatccgc agcgagggga gcgagaccga gacgccgcgg
    15061 gggccgaatt catcggtcat gcggcggacc agcggcgcga tctcggtata gtcggccgag
    15121 ctgagcgaga ggagcgaaat ctcgtcgtag ccggtgttct cgatgccctg gcgcgcgatc
    15181 tcgacgatct gctcgagcga gcgctccttg accgggcggg tgaccatgcc ggcctggcag
    15241 aaccggcagc cgttgacgca gccgcggcgg atttcgatca cgaggcggtc gtgcaccgtg
    15301 cgcagcagcg ggacaacggg cttgatcgac cccaggtcgc cgcccacgtc gaagtagcgc
    15361 ttgtggatcc gtagcggcag atggtcggcg tcgggggcgt cggcgcgccg ggtgaaaccg
    15421 gcgatcgtcc cgtcggcgtg gtaggtgaac tcgtagaatt ccgggacgta gacgccgggg
    15481 aggcgcgcca gctcgcgcag aatgcgggtg cggtcccagt ccgcggcgag ggcgcgctcg
    15541 atcagcgcgg tgatctccag cacgatttcc tcgccgtcgc ccaggacgaa cgcgtcgagg
    15601 aagggagaaa tcggttcggg ggaccacgct ccctcgccgc cgccgatcag gatcgggaag
    15661 gcggcggtgc ggtccttgct ctccagcggg atgccggaca ggtcgagcat cgtgaggatg
    15721 ttggtgaagt tgacttcgtg ctggagtgtg aagccgaccc agtgaaattc ggcgagcgag
    15781 cggcgtgttt cgagggaata gagcggcacg ccgtgctcgc gcagcagcgc ttcatagtcg
    15841 ggccacgggc agaaggcgcg ttcggcggcc cagcccggct ggcggttgag ccgttcgtag
    15901 agcaggcggt agccgtgata cgacatgccg atctcataca gatcggggaa ggcgaggcag
    15961 gcccggacgc gggcgcgagg cttgacgatc tgattgaact cgccgccgac atagcgcgcc
    16021 ggcttgccga cacgcatcag cagcggctcg atctgttccc acgcgaccgg catgcgattt
    16081 ttcctctggg ccggcttcgg ccggcgcggt aatgcaaacg gattgggcgc gttccgtatt
    16141 ctcgtcgttt tcccggtctg gcgcgccctg ccctcacttt acatggcccg gtgcgcggga
    16201 ggaagcggga agtggcgcgg aaacctggcc gagcgcgacc gcgagccgat caacatagtc
    16261 cagggccacg ggactcgggg cggcgtcgcc cgtgtttcca aagagggatg tttggggtaa
    16321 aatcatgccg gtggccggcg acgccggctg cgaaacctgc gtgcggcgcg tgccgcgtgc
    16381 gttgaagcga aggaaaatgt cggagctgat gaacgagacc ctggcgcggc tgggcgactg
    16441 gagcggacgg aaggtcgtcg tcgcgatgag cggcggggtc gactcggcgg tggcggccgc
    16501 gctgctgcat cgcgcggggg ccgaggtgat cggcgttcac atgcgcacct ggcattacaa
    16561 ggggtgcgag gcgaacgacg ggctggccac gtgttgctcg ccggccgatg cgcgcgacgc
    16621 gcggcgtgtc gccgatcagt tcgggttccc gttctacgcg atggatttcc aggtcaattt
    16681 ccgccaggcg gtgatcgagc cgttcatccg ggactatctg gacggccgga cgcccaatcc
    16741 atgcgtgcag tgcaacaaca agctgaagct ggggagcctg ctggccaagg ggggggccta
    16801 cggcgccgaa gcggtcgcga ccgggcacta cggccagatc gtgacgaacg agcggaccgg
    16861 gcggcatgag ctgcgcgtcg gcggcgacgg cgagaaggac cagacctact acctgttcgg
    16921 cctgacgcaa ccgcagcttg cgcggatgct gatgccggtg ggccacctga gcaagcccca
    16981 ggtgcgcgcg ctggccgagg agctgggtct gcatctgcac gccaagcgcg actcggtcga
    17041 gatctgcttc gtgaccgaca atgactaccg gcggtttctg cgcgaggagg gggcggtcga
    17101 cgaggcgcgg ctggcggggg cgattgtgag cacgcgcggc gcggtgctgg gccgccacgc
    17161 gggcgtgcac tgtttcacga tcggccagcg caaggggcta gggctcgccg cgccgcggcc
    17221 gctgtatgtg gtcgacctgc tgcccgagac gcagacggtc gtggtgggct acgaggagga
    17281 tctgctggcc gagggcctgg agatggacgg ggtgaactgg gtcgcgcgcg agccgtcgcg
    17341 cgagccgttc cgcgcgctcg tgaagatccg ctaccgcagc ccgggttacg cggcgacggt
    17401 cgagccggac ccggaggatc tgtcgcgggc gcgggtgctg tttgatgcgc cgcagcgggc
    17461 cgtcacgccc ggccaggcgg ccgtagccta cgacgcgccg gcgggccgga cggtgctggc
    17521 gggcggctgg atccgccggc gggtgatgaa ggaacccgcg ccgggcgaat ccggtgaatc
    17581 gggcgcccgt tgacctgccg cggctgaatg cctttccgat gaagctttgc atgatgcgaa
    17641 tgacgattga gcaggcattg atcggcggcg ggcgatagcg gttccggttt gttgtcatcc
    17701 cccttcccac gcaattgaaa gcagtggcaa tttcactgcg ggctcgctag aagtggcgcg
    17761 cctgctccgc gcgtcagcgg ggcgtcactc cgcctgaaag agaacgttca tgagcaaggt
    17821 ctttcgagtc ggcacgatcg gcgtcggcta cacgtgcaac gtccatgcgc ccgggtggaa
    17881 ggagagtccg cacgcggaac tggcggcgct ggccgacttg aacgaagagg cgctcaaccg
    17941 cgtggccgac cggctggagg tgaagaagcg ctacacgacc gcgcgcgagc tgatcgaaga
    18001 tcccgagatc gacgtggtgg acatctgcac gccgaacatg acgcacgcgc cgctggcggt
    18061 ggcggcgttc aaggcgggca agcacgtgct gtgtgagaaa ccgctggcgc ccacgccggc
    18121 cgagatccgc aggatgatca aggcgcgcga cgccgccggc aagctgctga tgacggcgca
    18181 gcacatgcgc tacctgggca cggcgcgggc gatgaaggcc gagatcgaac gcggcgtgct
    18241 gggccggatc tatcacgcgc gggcgtggat cctgcgccgc tgcggcgtgc cgctggggcc
    18301 gggcttcatc cgccgggagc tgtcgggggg cggggcgtgc atcgacatcg gcgtgcacat
    18361 cctcgacctg acgctgtggc tgatgggttt tccccggccg gtgacggtca gcgggttttc
    18421 gcgcaacgag ctgagcaagc ggcccaacgc gttcagttgg ggccgccagc cgattcccga
    18481 atcgatggac gtggaggatt tcgccggcgg gttcgtgcgt ttcgaggggg ggcagacgct
    18541 gatgatcgag atcagttggc tgctgcatca cgatacgccg ggcgaggtgc agcgggtgtg
    18601 gctctacggg accgaggggg gcgccgagtg gcccgccaac aagctgatcg cggacgacct
    18661 gccgacgcgg caattgattg atcgccagct gatcctgcac gacggcggcg cgccgcacgc
    18721 gctcgaatgc atcgatttcg cgcgcgcgct ggcgcagggg ctgccctcgc cggtgccggc
    18781 cgaagagtcg ctctacgtgc aggcgattct cgatgggatc taccgcagcg cggagcaggg
    18841 ccgggaggtg aaggtgaact tgtgagaccg gacgacaata taccgggttt cgagagccga
    18901 ggacgaggga cgagagccga gagccgaatt ccgggagggc cttcgggggc gaggacgaga
    18961 gacgaggggt gatattggag tgaggggcgg atcagaggcc gcggtagaag tggacgctga
    19021 acaggtggtc gtcgtcgagc cagacctggt ccaggcgcag accggcatgc agggccatcg
    19081 cggcgaattc gtcgatcgtg tatttatagg aactctcggt ccagatcgtg tcgcccagat
    19141 tgaagcggaa gctgcggccg ccgacctgaa cgatctgtga ggcgaggctg gccaggtgca
    19201 tttcgatccg gccggcctgc gggttgtaaa aggcgtaatg ggtgaagcga tcgaggtcga
    19261 agtccgcgcc gagttcccgg ttgatgcgcg ccagcaggtt caggttgaag cgcgcggtgg
    19321 cgccgcgggc gtcgttgtaa gccgggacga gcaggccggg gtccttcttc aggtcgacgc
    19381 cgatcagcag gccgccgttg cggccgcagg tctggcggac gcggcgcagg aagcgttcgg
    19441 cctcggcggg atggaaattg ccgatggtcg agccggggaa aaagacgacg cgccttgccg
    19501 cgtcgaacgc cggttccggc agcgcgcagg gctgggtaaa gtcggcgcag accgggtgaa
    19561 tccgcagatg ggggaagtcg gcctggagcc ggcgggtggc cgcctccaga ctctggcggg
    19621 cgatgtcgag cgggacgtag gtgcaacggc catcgagttg cctgagcagc aggcgcgtct
    19681 tgcggccatt gccgctgcca tattcgatca gggtcgtgtc cagtccgatg cactcggcga
    19741 tttcgtccag gttttcgcgc atgatcgcca gctcggtgcg ggtggggtaa tactcgggca
    19801 gctcgcagat ggcatcgaac aggccggcgc cttcctcgtc gtaaaagtat ttgcagtcca
    19861 ggcggcgggg gcgctggccg aggccggcga ggacatcgcg gaggaaggcg aggcggccgt
    19921 cttcgaacgg ttcggtctcg gcgtacagaa gggcggtgct catcatgcgt cctttgccag
    19981 ccggaggccg ctgaactgcc agcgcgccgc gggcgggaag aaattgcgat aggtgggccg
    20041 gatgtgggat tgcgccgtgg cgcaggaacc gccgcgcagg accatctgat tgcacatgaa
    20101 cttgccgttg tattcgccga gcgcgcccgg cgcgggctga tagccgggat agccggcgta
    20161 ggggctcgcg gtccactccc agacgtcgcc gaacatctgg tgcagccggc gggagggttc
    20221 gtcgagtgcg gcgccgtccg aacggggatg gaacgcgccc gactcgacga agttgccgtc
    20281 gagcgggagc gggcgcgccg ccagttccca ttcgaattcc gtggggagcc gcgcccccgc
    20341 ccagcgggcg aaagcgtcgg cttcgtaata gctcacgtgc gtgaccggct cgtgcggatc
    20401 gaccggccgg tccccgagca gcgtgaactg gcgccactcg ccctcctcct catgccagta
    20461 gaggggggcg ctccactggt tctcgcgggc ggcggcccag ccgtcggaga gccagaaagc
    20521 gggttggttg tagccgccgt cgcggatgaa gtcgatgaac tcgccgccgg tgaccgggcg
    20581 ggaggccagc gcgaacgatt cgagatagcg ccggtggacc ggctcttcgt tgtcgtaggc
    20641 gaattcgaat ccgagcggat cgaggtcgtg gggccggctg tagccgaaac tgtgcagccc
    20701 gccatggaat tggaaccatg ccagcggcgg cgcggacgcg gccggcgagt cggcccccgc
    20761 gaggtaggcc ggccgcaggg ggttctgcgc caggacatgc ttcacgtcgg tcagcatcag
    20821 ttcctggtgc tgttgttcgt ggttcagacc gagcagaatg atcggccaga tgcggccgag
    20881 ccgtctggcg ccggccgact caagcaggcg cgccatcgcg tcgtcgacgt gacgccgata
    20941 ggcgaaagtc tcggcgacgg tcgggcgcga gagcatgccg cggcgagggc ggcaatggcg
    21001 cgggccgagg gcttcgtaat acgaattgaa cagatagccg tactgcggat ggtgcggctg
    21061 gtactcggcg ccggcggcgg ccagcacgaa tgtttcaaag aaccaactga cgtgcgccag
    21121 atgccatttg gtggggctga cgtcgggcat cgactggatg accatgtcct cgacctggag
    21181 cgggcgggcc agttcctcgc tgaaccgccg gacgcgccgg tattccaggg cgagcgcttc
    21241 gcgttcgagc gcggtggaat gaagggcagc cggcgagggg ttggataagg ccatcatgat
    21301 gactccgcag cgaagcccat gcaccgtgcg gcgcggggag cgcacgggaa atcgagcgaa
    21361 acctgaatta tccgagatat tcctgaaccg gcccaagggg ccgcgggcga gtagactttc
    21421 agtcgcctgg cgtgagacag agcacgtgac gtgccagagt cttgaggcgg ccaggccggc
    21481 cgtggcacgg cgtggatttg ccggccagcc ggaggggtcg atcccatggg cgggagcgcc
    21541 gcgcgcaagg cgccgcgacg gagcgggacg gaacgcctgc cctcatcccc cgcggggcgc
    21601 ggggggtcgc atcccgccgc cgggttatgt aaaacttgca tgtccacggc cggccgccgc
    21661 gcggcgggac ggtctgtgag ttgctcatga accggaggtc acaacgtagg gacaaattgg
    21721 tgggttttcg agagccgagg acgagggacg agagccggga gccgaattct ggggaaggcc
    21781 gtaagggacg agggacgaga gacgatattg gagtgaggtt gcccatgcca acccccgccg
    21841 aactattaca ggcgctggaa agcgagatca cgtgctgccg caaatgcccg cggctggtcg
    21901 cctggcgtga gcaggtagcg cgggtcaaga cgcgccggtt cgccgactgg gagtactggg
    21961 gccggccggt gccgggtttt ggcgaccccg cagcgcggct gctgatcgtg gggctggcgc
    22021 cggccgcgca tggcggcaac cgcacgggac ggatttttac cggcgatcgc agcggcgact
    22081 ggctgtttcg cgcgctgcac cgggcggggt tcgccaatca gccgacgtcg gtgcatgccg
    22141 gcgacggact gcggctgacc gactgcttca ttgcggcgtc ggtgcgctgc gcgccgccgg
    22201 agaacaagcc gacgcgcgag gaattcgcca actgccatcc gttcctggcg cgcgaaatgc
    22261 ggctgctggg gcgcgtgcgc gtgctggtcg ggctggggcg gatcgggttc gacgaggcgc
    22321 tgcgcacggc ggccgagacg cgcggggcgg tctggaagcg caagcccgcg ttcggtcatg
    22381 gggtggtcca tcagctcgcc gagggcctgt ggctggtggg ctcgttccat ccgagccagc
    22441 agaacacgtt cacggggaag ctgaccgagc cgatgttgga cggcgtgttt gcgacggcgc
    22501 gccgcttgtt ggcggggtga ggcacggcgc gggggtgagg ggtttacgac ctggcgatca
    22561 acttttgcgt caagcaagct ggcgggcgtg ccgatacgat gcgcgaagga tagcgcctgt
    22621 cccgcgacgg ggaatctgcg cgaagctgcg gcctgttttt ctgggaaggc cgttgggggc
    22681 gattacgaag acgagggacg acgttggtgg ggggagcaga cgcgcgcctc cgccgttaat
    22741 tcctgatgca tcaggcagga tttccgccta ggattggggg cgttcacccc ttggccagta
    22801 cagtttatct ggagcgaatc atcatggacc gttccgcggc ctgcattgga cgcgatatga
    22861 cccgcgccct gctgttgtgc gtgttgacct gcctgcccgg atgggcgccg cggctggcgg
    22921 cctacggcga cgcgcaaaac ctgatccccg agttgcggct ggatcaggac gcgatcaatg
    22981 ccggcgaagt tcccttcgaa acgctgttcc acacgggaat gcacttctat accaaccggt
    23041 tcacgccggc cgaccactac ggcgaggcgc ccgacgggcc gcgcatgtcg caccaggcgt
    23101 ttcccggccg cccggcgctc cgcttcctgc ggttcaacgg cctggacgcg cagacgtgcc
    23161 tggaatgcca cgcgctggcg gggatggcga gccggcccga agccggctcg ccgctgatgc
    23221 aattcccgcg cgaccccggg gtgccgggct cggcgggcct ggcggccaac gtgttcgtgc
    23281 tggagaattt tccggcctcg accgccgggt ttatccgcaa cccgccgcat aatttcgggc
    23341 tgggctatgt gcagcggctg gccgaggaga tgtcgctggc gctgatgagc cagcgtgacg
    23401 ccgcgctggc cgaggccgcc gacgccggcg cgacggtcac ggtcgcgctg agcgccaagg
    23461 ggatcagctt cggcacgctc gcggccggac ccgacggaca gttgaacctg gaggggctcg
    23521 aaggggtcag cgaagacctg gtgatccggc cgtttcagtt caaggggatc gccagcacgc
    23581 tgcgcagctt cgtcgccaat gcgatgaatt ttcatttcag cgtgcaggca cgggagctgc
    23641 tcgaccgcgg ggtgattccg aacgacaacc ccaacggcac gctgccggac gatccgcgcg
    23701 acgagctgct ggagggggat gtgacggccg tggcgacgtt cgtcgcattc gtccgcccgc
    23761 ctgtcgagat gatcgagccg cagtatgcct tgcaggcgat gcagggtcgc gtcgtctttg
    23821 aacaggtcgg ctgcatcgag tgccacgtgc ccgaactgac gatcgacgat cccaacctga
    23881 cgattgaaga tccgcgcgag cagctcaagg cctacaaggc gctgcgccag aaggggctgg
    23941 ccgccgaacc gcccgccgtg cccgcgctgg tcaacccgga gccggacggt tcgatcctgc
    24001 cggtcgtgct gctgtatgaa cagcgtcgcg ccgcgctggc cgcgggcaag agtcagccgg
    24061 ccggactgcc cggctacacc cgcaacctga acctgaacga cgggccggcg gagacgcggc
    24121 cgcggctgcc gcatgccgag gacggcagca tccgcgtgcc gctattcagc gacctgaagc
    24181 ggcacaagat gggggcggat ctggccgaca cgctggccca ggggaccgat tcgccggggg
    24241 tcgccgttcc cggcgacgaa tttgtcaccc ggccgctgtg gggcgtggcc gacaccgggc
    24301 cgtggctgca cgacgggcgg gcgctgacgt tgaccgacgc catcctgatg cacaagggac
    24361 cggggagcga ggccaacgag gtgatcgaga atttcgaaaa cctggagcag gacgccaagg
    24421 atctgctgca caagtttctg ctatcgctgc gcacgccgcc gctggaagcg cccgacggca
    24481 acgccgccga ggcgtggcgg gggtattagg tctcgttccg ctcaagaatc cgtccgtcga
    24541 ccctcgtcca cgtccacatg atcgtggttg atgcgagggg tcttgccgag agttctgggc
    24601 tacagttatc gtctggcatc cctgccggga cgcgggggat ggggttgccg cggggaccgg
    24661 tggtgtcgct gcgctcgacc accggctgcc atctggcatc ccctgtcggg atgggcggga
    24721 gagaccggcg ggggcgccgg tccgaaaccg gtggtggggc gtcggcaatc ctcataatcc
    24781 atagtcggtc aataacggaa cgatagagcg ggttttcggg agccgaggac gaaggacggg
    24841 agccgggagc cgaattcccg ggcgggccgt tgggggcgag gacgcgggac gaagttgttg
    24901 ttggggattg acaggccgcc acggtttcca gtacaacccg aaagtggggc aaagtgtctg
    24961 gcgttgcatg acttatgccg ccatgacgcg tgagctcccc ggttgttcct cctccggcgg
    25021 gacattgccg gcggggaacg aagcctatgg gtcggaccgc gactccgcca cgccggagcc
    25081 cggaccgcac gatcgttcat gcccccggcg cgcagcgggg cggaaagtgg tggtgtcgcc
    25141 atgttgagat cagccaacgc gaaaaacggc ttcgggccat acgcggcctg tctggccctg
    25201 ggcgcgatga tgatgttgct ggcgcccgcc gcgtggtcgc agggggggtt cgtggggctg
    25261 gccccgcccg atgcgatgga cacggtctcg gggatgtcgg tgggcggcgg cggcgccggc
    25321 aatgtggcgc tggatcgcgc acgggggttg cagcagccgg ggatgatggg gatgcccggg
    25381 ccgggcggcg gtccgatgcc gggcccgatg ttccccggcg ggatgtttcc gggtgatccc
    25441 ggccaggcct accagccccc cgaagcgctc gagccggtga tcccgacgat cagcgtgctg
    25501 gtggggaccc gcgtgcgcga tgccgtgacg aaggaactgc tggatgagcc ggtcgagctg
    25561 aaggtgccgg aaacggaaaa ggggaacttt tacgacgacg gcacgcacgg cgacgtgatg
    25621 gccaacgacg gcaaattcgc gatggtggaa ttcgccgacg acgtgctggg cgaagggagc
    25681 cagcgggcca aggaacagat ggtcaaggcg ctggtggtgg ccgaagatct gacgccgctg
    25741 gagttctatg gctttacgat catggggacc gaccagcgca cgccgcagcc gcgcgagaag
    25801 gcgtggcaga tgataccgga tccgaagggg ggtccggggt ttgtgctggc ggaggtggag
    25861 accgagcgtc cggtggagat gccgaactac cgggaatggc gcaacgcgcg cgacgcgaag
    25921 gtcaaaaatg actggtcggc ccggttcctg cgcaactacc gcgtcaaccc cgacgatatc
    25981 cgcagcgagt tctatcatct gtatattccg cacccgccgc agccgcccga agtgcggccg
    26041 ccggtgcagg aggggtggat gccgttcagc gatccgcagt cgctgatgcg ggcgcagagc
    26101 ggtgagttca acacgggaac gacggggggc ggttatggca cgcccgggat cacgggcggc
    26161 tatccgctgg gccagggatc gggggtcacg ggccagccga tcgggaatgc gtcgagccgg
    26221 tacttctagc agggaaaagg actgaaggca cgttagggac ataagaactg agggcgttcg
    26281 agagggacga ggaacgggag acgaggccag aataaagacc ttgggtcggc cttcgggggc
    26341 cgatcacgag gtcgagggac gagggacgac actagagcgg gggcggaaaa gcgatggcgc
    26401 tgaatcctca agacgtcgac gagatgcacg agacgacgaa gaagctgctc gagctgtgga
    26461 tgcggaccaa gctggtcttc ctcaaggcgt tcagcggcga gccgatcacc caggagcatg
    26521 aaaacgccta cctgcagctc aagagcgaga tttcgcgcct gtaccgggtt atttccgaca
    26581 agctgacgcc cgggctgatg ttcgacggcg acaagatgct cgaaatgctg aaaaacgcgg
    26641 tgacgatgga acacctgcaa cgccagtcgc ccgccgagcg ctcgaacctg atcagcgcct
    26701 ggcaccggat ctatatccgg atgacgcgga cgctcggcgc gctggaggtg atgcagtcgg
    26761 ggtattaccc gcacctgcac cgtgcgctgc tgacgggcaa gggggccggc aagggcaagg
    26821 gccggtgggg ccggtcggcg aaaaaggcgg catgaacggg ggcccgccgc gatcaaatca
    26881 gactggaagt ttatgggctg atgggccaca cttgcggggc catggaaggc ggcgggcggt
    26941 tcattgcgcg cgccgtggct ggctgacgcg cgccgcacgc cggccagatc cccgtccgta
    27001 gcgggcgaga ccatggcgcc ccgggaagcc ccgaagccgg accgacggcg gcgcggagcg
    27061 gccgctttcc cgacctgatg gctgtggagg atgactgttg tgaagagcct gaactcgctg
    27121 cgcaaggggc tggggctggc ggtcctgggc atgctgctgg tgacgctggc cggttgcaac
    27181 aaaccgctgg catccttccg actccggggg gccgagaagc gggtccagga ggccgaggag
    27241 aagcaggcgc agcagcacac ggccgagctg ctgcagcaga cgcgcaacgc gatcaatacg
    27301 acgcagaatc agttgaatca gggcgacgcg gtggcggcca aggagtcgtc ggccgaggcg
    27361 gcgcgcctga gcaaggaact gctgcagcgc acgaccgagg cccacgcgat tttcctgcgg
    27421 gaccaggcga acatctggat cgaccgcgcg cggacgaacc aggcgcagca ggagaacgcc
    27481 gagctgttcg cgcagatcca ggagaacaat gtcgagggga ccgaggcgtt cggcaaacag
    27541 aagtacgaca aggcgatcca gatcttcggc aaggtcgtcg acgacgtgca gtatctgctg
    27601 agcgcgctgc gcaagaaggc gaccgacgga ctggccgagg ccgaatcgct caaggaggag
    27661 ctgattgccg agggggcgcc ggagcacgcg cctgaattta tcaacaagat cgaccagcag
    27721 attacgcaga ttcgcgacaa catcgagcgc gagtacaact accgcacggc cctggcgatc
    27781 cgcgatcagg cgcgtcagac gaagcaggaa gggatccagc agaccaagaa ggtcaagagc
    27841 gacaagcaac tgaccgagat tgagaacctg ctggacgagg cgacgacgct gggggcggag
    27901 acctacacct acaacttgtt cagcgcgatc acgaaggaat tcgagaatct ggtcagccag
    27961 ttttatgagg aaaactacga cacggtgctg acgcaggcgc ccaagctcaa gccgcaggtc
    28021 gaggaactga tcctggagac caagcgggtg gcggccgaga cgaagatcaa ggaagtggaa
    28081 ggggcgatca acagcctggt ggcgatggag gcgcgcggct acctgccggg gcgggtcgag
    28141 cagctcgagg cgctgctggc cgacgcgcgc gagcaatatg agcaggaggc gtatgtcgaa
    28201 tcgcgcgaga tcagcgaccg ggccctggag gaggaacaga acatcctgca ggagttcgac
    28261 gatctggcgc agcagcacat cacgacggct tcggacgagc tggcgacggc cgaaggggtc
    28321 tatgaaaaga tggagcatat cttcctgcgc cagattcccg gcccgtggga aggcgacgcg
    28381 ctggcgctgg agaacgccaa gcaggcgctc aaggaggagc tgcggcggcg tgtgaacaat
    28441 gcgcgggtga atctggggat ggcgcagctt cagcgcgagg agaaggactt cgaccgggcg
    28501 atcgagatcg cgcgcgacgt ggccagcgag gccgaggacg tacgccagca gacgttccgc
    28561 gtcgtcgcgc acaacgcgat cctcgacctg tccaacatgc tctcgcagta cgaggggcag
    28621 gggggccgcc agtatgcggc cagcgagatg gacaaggcgg tcgagatgct ggagcagagc
    28681 aagcaactgc tggcgaccga acagtatcgc gaggcggtgc ggcgcacggc cgacaccaag
    28741 gcgcagatcg aggtgctggt gcaggaactg gaacgggtgg cggtcaaccg gatcgagagc
    28801 gcgcagcagg cgctggcgca ggccaaggcc gaccgggccg aggaatacga gccgattgcc
    28861 ttcacgcagg cgctggtgga gttgcaggcg gcgcaggagg cgctggcagc cgagggtcgc
    28921 cacatcgcga tcgaggcggc gatccaggcg gagaacctgg ccgccgaagc gtcgaccaat
    28981 gcgctgcgcc agtgggtgca ggagctgatg gccgaagccg acacgctgat caacaacgcg
    29041 cgcgaagccg aggccgaccg ctacgcgccg gagaaactgg accgggcctt cgcgatccgg
    29101 cgcaacttgc agacgcttta cgatcagggg cagtatcgcg aggcggtcga tgtcggcgcg
    29161 cagacggtgc agcaggccca cgaggctctg tatgccaagg tgatcgaggc ggagaacgcg
    29221 atcgccaagg cgaagcggtt cgagggatgg gagtttgaaa acgcccgcct ggccgacgcg
    29281 atggtgagcg ccaaatatgc ccgcgagatg atggccgagg ggcgcttccg gctggcggaa
    29341 cagcatgcct ggaacgcgct ggtgaaggcc gaggaagcag cggtcaacgc gcggcgcgac
    29401 ggcttcgaga cgcgcatggc cagcctggcg gcccgcgtcg aagacgccca gcgcaagggc
    29461 gccggctact atcagacgca ggatctggcg tcgctgctgg ccgagatgaa tcgcctgcgg
    29521 atggagttcg atccgcacga ctacgaggat tatgcgcagc aggtcgacct ggtcgaggcc
    29581 aagctgatcg cgctgatgga gctgaccccg gacgtgctga aggcgctggt gctcgacatg
    29641 agccagcgga tgaccgagct ggagcagcgc ggcgcggcgc tctacatgcc gaacaagctg
    29701 gcggaggtcg aacgcaaact gaagtacgcg cagatcgact accaggccgg caagtatcgc
    29761 cccagctacc agaacgcgaa ggacgcgtgg gccgtgctgg atgagatcga gcagaacctg
    29821 gaggagcgcg agttcgatgc ggcgctcaac gacctgatgg tcgagctgtc ggatcagatc
    29881 cgggcgttcg cgccggtgct cgacatgggg gcgaacacgc tgctggagct ggtgatcggg
    29941 ccgcaggggc aggcgcgggc gacgtcaatc ctgggcgtgc gctcgcccac ggatctgcgc
    30001 gattcgatca ccgagatcgg cgcccggatc cgccggatgc aggcgccccg ttcgcgcgag
    30061 acgctgcagc aggagatgct gcgcatgatg gagatcgcca agacggccgc gtcgaatttc
    30121 gagaagatgc tgatcatgga ccaatacagc cgcgaccagg cgcgggaaat cgtccagacc
    30181 gcatttctgc agatgtatca ggcgcgggcg cgccagcagg aactgcagcg gatgatcgag
    30241 tatcccaatc cgcagttcaa accgcgcggc gtcgaacgcg tcgtctcgtt ccaggattac
    30301 tgagccggag caggtgaggg atggattttc tcaagggcgc caacgccgtc tatgcggccg
    30361 aaatcgtgac cgtgctggca attctgacgg tgctgggtta ttacttgtat tcgatcggcg
    30421 tgttcaagaa aaagtagctg atcgttgccg gggggcgcgg cggccactct cgcgggcgcg
    30481 gccgcgcccg ccgttccatt gagcgattga cgagagcgca tggcactttc caaacccgcc
    30541 cctccgcgcg cggccggcaa gcagaagaaa gccgatgtcc gtttcaagct gaccaaggcg
    30601 cagaaggcct acgtcgagca actggtgacg ctcgacaacc ggatcgcggt ctgccgcgaa
    30661 tacatcgcgc tgtggatgaa ctttttccgg tttttcgccg aggacatgac gcgcaaggag
    30721 atcaccgccg ccgaggaaaa gcagttcttt caggtgatga cggcgctgac gcgcaagcac
    30781 ttcacgttcg tcgagctgat ggccgagacg ttcgaccggg gcaacgacat catcgccgtg
    30841 ctgagcaccg ccgtcagcct gacgcacatc cagaacatgc cggagaatac ccgcggcaag
    30901 ctggagctgg actggcacaa tctcttcctg gacatgaacc ggacgctggg ccggttgctg
    30961 cgccgcctgc cgggcaacat gacgctgagc gaggcgctgg cgtcggtcaa cgccaagccc
    31021 gtggcgcccg atccgggtgc ggcccgggcc gacggcaagg gcgccaagcc ggcggccgcc
    31081 gcgggggcat aagccggcgc ggcgcgcggc ccgggcgcaa ttccccatca acagattttc
    31141 ccgtttgcgg aggtccgcga tgacgctgtg gcacgataca ggggcgcggc gcttcggctc
    31201 gcgcgacgaa attgaggcgg cggtcgcccg tggcgaatcg ctggccggcg cgtgcctgga
    31261 ggggctcgac ctggcggggg cgaagctggc cggcgccgac ctgaccggcg cgcggctgag
    31321 cggggccaac ctgaccggcg cggtgctggc cgacgcgacg ctgacgcgcg cccggctgga
    31381 cgaagtcgtc gcgccggaag ccgatttctc gcggatccac gccgaattcg ccgtgttcgg
    31441 gctggcgcgg ctcgccggcg cgcggtttca cgccgccgac ctgcgccgat ccaattttgt
    31501 gggcgcggtc gcccccggcg ccgacttctc ccaggccgac gtctactacg cccggttcgc
    31561 cggcgccgat ctgtcgcagg cctgcttcac gcaggccaac gtgcagcggg cggtgatccg
    31621 ggcggccgat atccgccatg cgcggttcga gggggcgcgc ggcaaggcca cgttcgaccg
    31681 gaacacgcaa tagacgaggc gacggcggtc gattgcgatt gcgataccga taccgatagc
    31741 gatgggttgc gaacatgctg ctgcgtcatt tcatgcttga tgtcgggccg caggaggcca
    31801 actgctacct gctggccgat ctggcggcga agaccgggat cctggtggac gccggggcgt
    31861 tcgaaccggc gatcgtggcg ctcgcggccg agcatgacct gcgcatggcg gcgatcctgc
    31921 tgacgcacct gcattgggat cacgtcgacg ggctgccgga atatctgcgc cactggccgg
    31981 cggcgcgcgt gatcgcgccg gcggcgctgg cgacggccga ggtcgagcgg acggcccctg
    32041 gcgaggagct tgaaatcgaa ccgttccggt ttcgcgtgct gcgaaccagc ggccacacgc
    32101 ccgaatcggt gtcgtattac tgcgccgagc atgcgatctg tttcgtcggc gatgcgctgt
    32161 ttgccgggtc gatcggcggg acagccgacg aggagaaatt ccgcgaggag accggctgca
    32221 tccgcgacca tctgttcacg ctgcccgacg cgaccgagct gctgccgggt cacgggccgg
    32281 tcaccaccgt cgctatcgag cgcgcggcca accccttctt gaagccgggt ttcggtcgga
    32341 attgacgcgc gccgtagtcg aaattcgccc ccttcgggga cgggggtttt ccgtgtcggc
    32401 gtccccaggt tgcgttgcgc tccacctggg gccattcaat taccgccccg ctggggcgac
    32461 tcacgcggcc ggctgttgac ggggatttcg agggaccaga tttcagtgag ggacgaacgc
    32521 tagcgtgagg ccggccatcc aggatttgcc aaccgatcgt cccctgcacc ggctcggctc
    32581 gtggatcgcc cttggcgtct ggctcgcgct gctggcgacg atcctgatcg accggatggc
    32641 gcaaccgtgg cggccgctgc tggcgtggtg cggcatcgcg ctgctggcgc tggtcgactg
    32701 cccctcgccg cggcgcgagg ggggggcgct gcgccggcgg ctggcggtcg ccgggctggg
    32761 ggcgctgctc agcacgatcc ttgccatcga catcggcatg ctggtcgacc gcgcgccgca
    32821 ggcgatcaac gtgtggtatc tgcgcgcgcg ccatgagctg tggcacgatt tctggaccga
    32881 tccgagtccg ctgctgatcc tcgccacgtg gtggttcgcc gccccgttcg tgatcgacgc
    32941 ctggcgggcg cggcgcgaac cgcgcgcgcg gacggccgaa gcgctgacgc ggcgccacgc
    33001 cctctggctg ctggcctggg cgttctggat cggcgtggcg atgttcgcct cgatccggcc
    33061 cgaccgggcg tattaccggt ttccacagga gatggggctg cacgcgccgc tgttcctgtt
    33121 ctggatacgc cgcgcggggg cggcgcccga cctgcagcgg cgactggcgc gcatcgcgcc
    33181 gtggatgatg ggggcggcgc tactggcggg gctggtcgtc gcgatcgctt acgtgaccgg
    33241 cgactggaac cggcaggtgg cgatcgggcg ggcggagatc tggcgcaccg aactggtcga
    33301 gcgccagtat atcaacggct ggcagtggcg gctgaacttc ccgctcggtc atcacaaccg
    33361 gatgggtttt ttctcgatgc tgacggcgct gctcttgttg atggcggccc gccgccgcgg
    33421 cggctggatg cggtgggtcg cggtcgccgc gatgctggtc gccctggcaa cgctcgggtt
    33481 ttcgctcaac cggggatcgt ttctggcgct cgtggcgggt ctgacggtca tgaccgtcct
    33541 gtggctgggg cggcgggcgg cgtggctgct gctgttgctg ccggcggcct ggctggcaat
    33601 gagcgacctg caacgccagc aggttctgtc gctggttgac cgcgaaacct atcggtcgcc
    33661 gcaaagcacc gtggtcacgc ggctggatca ctggcgcctg gcggcgcggc tcatccgcga
    33721 gaacccggtc ttcggcatcg gctacagctg gcggcatttc gaggagtacg ccgaggacta
    33781 tgcgaagcag gtgggcgcgc cggtgtatga aaaggcccac gcccacaact actggctgca
    33841 gacggccgcc gagaccggcc tgccggggat gctgctctat ctggggtgga cgatcgggcg
    33901 gtggagtctg ctgctggcgc ttgtccggcg gcgccgccgg ttgccggccg gcgagcgcgg
    33961 tctcatcgag gggtggtgcg gcgtcgaggc cgcgattcag gtctactgcc tggggaatct
    34021 gccgctgcgg ctcggtctgg ggctgctgac ctgggggatc tgggcggtga tgtgggtcga
    34081 tctggtgcgg ctgtggcgcg ccgcgcagga cccagcgggc gtgccgagcg agacgagcta
    34141 agcgcggcgc atctcccctt tgcctcgccg ccgggattgc gggtatcgta ggggggcgag
    34201 cgaccgaatc cggggagagc cccatggcca ggaacgaagc cgacggcgtc aagctggttt
    34261 gccagaaccg caaggcgcgc cacgagtacc acatcgaaga gacctgggag gccgggctgg
    34321 agctgcgcgg caccgaggtc aagagcctgc gccaggggaa aggaagtctg caggaggcct
    34381 acgcccaggt gaaggggggc gaggcgtggc tcctgcagtt tcacatcccg ccgtatgagc
    34441 agggcaaccg gttcaatgtg gacccggtgc ggccgcgcaa gctgctgctg caccggcgcg
    34501 agatcggcaa gctggccggg gcggtcagcc gccagggata cacgctggtg ccgttgcggg
    34561 tgtatttccg gcgcggccgg gccaaggtgg aaatcgccgt ggccaagggg aaaaagctgt
    34621 acgacaagcg cgaggcgatg aaggaacgcg atacgcggcg cgatctggac cgggcgctgc
    34681 gcgaccggcg cgtttaagtt tttgcctgac gcgccgcgcg gggctatagt ggccgaaact
    34741 gcgtcgggtc cgtccggcgg gggaggggcg cgggccgtcc ccatccgtcg gcgcgcgacc
    34801 gatcggcgcc ggagctcatc caaccagcac atgcagaacc cgccgtcgag caccccaccg
    34861 cccgcgtccg tatccctgtt ccggttgccc cactgggtcg gctacggctt gtttgtggcg
    34921 gttgcgctgg ccctcttttt caccgccgtc cactcctatc gccagttcgt cgatgactat
    34981 cagtacaacc ggccgcgcta cgcggcggaa cgcgcgctcg atctgctggc gcagaaccgg
    35041 acggacgagg ccgcgatcga agtccaggtg ctgatgcgcg accgcccgat ctacaccggc
    35101 ctgctcaacc aggagatgag cgaggtctat gagggaccgg tcttccgcaa ccgcgtgacg
    35161 accttcgagc ggttgaccgc cgcgctgctc gaccatgacc tgctgctcga ggccgagatg
    35221 gtcggctgga aggcgctgct ggagtttcac atcgcgagcc gcgcgctgga gatgttcgtc
    35281 ccctgggaac tgatgcacgc ggtcaaggtg ctgcgcgacg actggtcctc ggcgttccag
    35341 atcagcaagc tgctggcggc gcacggggtc aagaacaagg tgcgcctgcc caacgaaatc
    35401 cggcccaatc ccttcccggt cgatccggcg ccgttcgccg atttgaaccg ccagatgccc
    35461 gatccgctga tccaggggct gttcgactac taccagagcc ggctggcgcc gcggccgccc
    35521 gaggcgcgca acctgcgcaa tcgcgcggcg gcccagctcg ccaacgcgga aaaagccacg
    35581 gttatccccg gcgtgcagcg ccagcttcag gccttccgcc accggattct gctcgagggc
    35641 gatcgccggc ag
//
LOCUS       DAOPCO010000011        33267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1472_length_33267_cov_5.398470, whole genome shotgun sequence.
ACCESSION   DAOPCO010000011 DAOPCO010000000
VERSION     DAOPCO010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 33267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33267
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1472_length_33267_cov_5.398470"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLS90_01800"
     CDS             complement(<1..259)
                     /locus_tag="PLS90_01800"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPK01168.1"
                     /translation="MGLVPMRQILDEAAKGGYGVGAFNVNNMEQIQAIMEAANETHSP
                     VIIQASRGALKYSKMIYLKKLMEAAVEEYPHIPVALHLDHGN"
     gene            complement(852..3488)
                     /gene="ligD"
                     /locus_tag="PLS90_01805"
     CDS             complement(852..3488)
                     /gene="ligD"
                     /locus_tag="PLS90_01805"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HPK01169.1"
                     /translation="MSLKDYHRKRDFKKTTEPRGRTARTATGDRFVVQKHDASRLHYD
                     FRLELDGVLKSWAVPKGPSLDPADKRLAVHVEDHPVDYGDFEGVIPEGYGAGTVLLWD
                     RGRWHPRDKDPRKAYREGHLKFDLEGEKLRGGWMLVRMSGERGDDGRNWLLVKEQDAA
                     ARRGKAGRITDHEPASVETGRDINQVAAAKDRQWRSNRETADPDRKQKELRKQTTGRA
                     ADAAPRTKKAEPPDPARVKGARRGPLPADPRPQLTTLVAQPPRGDGWLHEIKYDGYRI
                     LARLEHGRARLLSRNGKDWTARFEAIATALRELEVDDALLDGEVVVLDEKGVSSFQQL
                     QNFTRQGKQAELCYYVFDLLHLNGYDLRRAGQLERKELVRRLLVRAGRKGRVRFSDHV
                     RGGGDEVLDNACRLGLEGIISKKADAPYAERRTRSWVKLKCGRRQEFVIAGYTDPGGS
                     RAGFGALVLGYYDEGGRLIYCGRVGTGFSVRVLKRMHEDLRRRERRQSPFDRGPKGAD
                     ARGVHYVAPELVAEVAFTEWTDEGILRHPSFQGLREDKQPKDVRRERPAATAADNGRA
                     RAGAPARRNRATESDEDMIAGVTLTNPERVLYPEQGLTKRQIAQFYVDIADWVLPYVV
                     NRPLSIVRCPAGRQKHCFYQKHVNDTVHPAVSGVEVQEKDGPKQYLMITGLEGLVALV
                     QMGVLEIHPWGARADRVDRPDLLVFDLDPDPAVGWEAVVTGARLMHDRLEELGLRSFL
                     KTSGGKGLHVCVPIERRTSWDDVKAFCRALAESITADDPAHYIATMSKARRKGRIFID
                     YLRNARGATSVAAYSTRARPGAPVSAPIRWDELTADLSPSQYTVENLRDRLNALKQDP
                     WDGFFDVRQSITAGMQRKLGMK"
     gene            complement(3513..4382)
                     /locus_tag="PLS90_01810"
     CDS             complement(3513..4382)
                     /locus_tag="PLS90_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HPK01170.1"
                     /translation="MARPIWKGNITFGLVNIPVTLYSAEKRNNLHFNLVDSRNRARVR
                     YERVNEVTGEEVPWSEIVKAFEYDGDDYVLLTDEDFQKAAVENSQAVEIESFVDLEAI
                     QYPYFDKPYYLVPGKKGEKGYVLLRETLKRTGKVGIAKVVIRTRQHLAALVPQGDGLV
                     LDLLRFQQELRDLDEFDLPTGGLKQYKVSDKELKLAEQLVEAMSAEWDPAEYHDDYRD
                     KLMAFIEKKARSGKGAEPLEEEPEEKDEQAEVVDMMELLQRSMKGGAKKEQAGKSARK
                     APAKKAARKRAAG"
     assembly_gap    4513..4612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4613..5072
                     /locus_tag="PLS90_01815"
     CDS             <4613..5072
                     /locus_tag="PLS90_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01171.1"
                     /translation="EAAPLFRGNPEERHRIVAAIGVVAAMSPAAKAALAARTADTLAL
                     AGAMPGTAVAAGGGAAGIGLGAGSLALLLLLAVGGGVFWMRSNAAPAAPAAIVELWGS
                     DTPATRGDNAPGPGGPRSRATPAAIPISPTCRRSPICSTRRATWPAGRRS"
     gene            5069..5773
                     /locus_tag="PLS90_01820"
     CDS             5069..5773
                     /locus_tag="PLS90_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01172.1"
                     /translation="MIDGVDHHGPLAERPLRGQSGRVWGGYCSARSTAASRGNSHHLA
                     IFHGALLAAERDPMLNRGFLDFVSHSRPAAADFPSPYTADTGSYVYTVFSSDPAGPAI
                     VDVRAGRLVARWNFRAGDPRFRILLQLDGSVWIRNDADLPALDRPGGAGDGPSFNLAE
                     LDWERVSADAEQNMNRLDDGGEVPLGPGAAVAADLARVTGMGVVLSRLADGLSSAGVP
                     TSVQLMELRLEGLAAQ"
     gene            6216..7094
                     /locus_tag="PLS90_01825"
     CDS             6216..7094
                     /locus_tag="PLS90_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPK01173.1"
                     /translation="MNRTRWLLCILLAGWLSLSLPDSLPGQQPNRDPGEDLEPEEAAL
                     PDFGEAGLSEPIPIDEAGAPIVEPTEGAEITSATLELLRELETFHREIDTIHVTFDQV
                     RIDPIFEDRIESQGELWFDKPDRFRANYADPRPMTVMVVDDTYYQYVPELEQVEYLTF
                     ESEADRNASLHWLLIGFGLKVDELAQRYAIRSSADDPALRTELGLEAAESKALFAVRP
                     RPAFEEATPFRELKVTIDKDRMLPEKVWYVDYNDAEMNVTMREIELDQRLDEDLFDVR
                     KAIPRGTTHIDMRSMQ"
     gene            7091..7729
                     /gene="lptE"
                     /locus_tag="PLS90_01830"
     CDS             7091..7729
                     /gene="lptE"
                     /locus_tag="PLS90_01830"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPK01174.1"
                     /translation="MSPFPAGPRRIALWLLALGLLPVAGCERVAPVRTLPSWVRGIYV
                     PMFENETFEPGIEQLATKMTQEEFLRDGRLDVVPERDADLVLKAKITEWLSKAGGSSG
                     DKITEDEKITMVVNVGLYEPFEDEPLAVLKPIRISSKFNIDVRSSDFEPEPDRRRALF
                     QALGRRIVFDTITGFPPMVRDLPAGVTLPSIPNPEDIEPDQSLLPRRGPDFD"
     gene            7953..8831
                     /locus_tag="PLS90_01835"
     CDS             7953..8831
                     /locus_tag="PLS90_01835"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HPK01175.1"
                     /translation="MTTPSPSPAPSTPPPAAQSPAAEALVAHDLWHQFQPNWWVLRGV
                     ELTFRAGELVGLVGPNGTGKTTLIRLLAGQAAPSRGQVTLGGRPLSKIGRLELARQIG
                     YLPQNVRSSFRLTAEEVVAQGRYPHQRGLGLLDARDHAVIRRVMDETRTLAFARRQIE
                     DLSGGERQRVLLASVLAQEAPFLMLDEPTSMLDIHHQVEVFQHIRRLARRGLGVLVIT
                     HDLNLAAHYCDRLVMMFEGKVEAEGAPEAVMREAVLRKVYETELIVDRNPVTGSPMVV
                     LLGNREEEREDRRWPC"
     gene            8819..9877
                     /locus_tag="PLS90_01840"
     CDS             8819..9877
                     /locus_tag="PLS90_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPK01176.1"
                     /translation="MALLTARRFLLVLAGYALFALAAMLAAPMFGAEPVNVPRALSQW
                     WETRGAAVRPYEVDILLHLRLPRILLAFLTGGALAVTGAVFQALLRNPLATPYTLGVA
                     SGGSFGAVLAFFLPAYLPWIAFELGPFNYVQCLSFAGSLGAMALIYGLARTGGRISTM
                     ELLLAGVTLGMIFSSLILAVRYFASPHIVVNMDRWLMGGLSVTGYGEVRSVLPFLAVG
                     LGVQLGLARALDQLGLGEELAQSRGVNVARLQALVFVFGSLAVASVVAVAGLIGFVGL
                     IVPHTARRLTGPDHRLLLPASLLAGGGFLVLCDTVARTIIAPTELPVGIITSLLGGPF
                     FIYLLVRSRRGGRMWGVD"
     gene            complement(9914..10426)
                     /locus_tag="PLS90_01845"
     CDS             complement(9914..10426)
                     /locus_tag="PLS90_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01177.1"
                     /translation="MEPWKPTSRQLDLLIALGLSAAYLIATFPWIYHDGFAEHDCYVM
                     GAGVVQATWQGTGLLEPMLYFPVSQPLYYLLFLNWPGPVAENLNGLIPLMNRLSWLAM
                     GVGVGLLYGIIRRVAPPAWAGIGVVAAFTTPTLFEMGTYGHPSSLALHASFLSLASLM
                     SLMSLMSLLI"
     gene            complement(10485..11438)
                     /locus_tag="PLS90_01850"
     CDS             complement(10485..11438)
                     /locus_tag="PLS90_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014259753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPK01178.1"
                     /translation="MRILVLDGYTLNPGDNPWDPVAALGDLEIHDHTPPEQIVERARA
                     AQIILTNKAPLSDETIAQLDALRLICVTATGYNVVDGAAARRRGIPVCNVPVYGTDSV
                     AQFVLALILNHCHQIARHDAAVHAGEWSRSPHFCFWLTPQLELAGKTIGIVGFGRIGR
                     RVGELAHAFGLRVLACSPSPGPEVAYPVRRVALAELFSLADFVTLHCPQTPATTGMVD
                     RALLARMKPGAYLVNTARGGLINEADLAAALAAGRLAGAALDVVSTEPIAPDNPLLRA
                     PNLVITPHMAWASLEARRRLMRTTAENIAAFLRGAPINVVN"
     gene            11652..12101
                     /locus_tag="PLS90_01855"
     CDS             11652..12101
                     /locus_tag="PLS90_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01179.1"
                     /translation="MDRDAFAARLAARLKKVRGKPIDQIPQGDYCYESHIPGGEDNDP
                     ELAACLKDGETLNAAYFVLWVEKHKERNCPYWEATDYGTVKCHYLEEEALGLDEEDYE
                     KALAQFGSEEELARNCTDNFCLADACRSCRLVLEALEAERRRESSEN"
     gene            12510..12586
                     /locus_tag="PLS90_01860"
     tRNA            12510..12586
                     /locus_tag="PLS90_01860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12544..12546,aa:Arg,seq:tct)
     gene            complement(13135..13547)
                     /gene="rnpB"
                     /locus_tag="PLS90_01865"
     ncRNA           complement(13135..13547)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS90_01865"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(13625..15832)
                     /locus_tag="PLS90_01870"
     CDS             complement(13625..15832)
                     /locus_tag="PLS90_01870"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPK01180.1"
                     /translation="MDKLLLPESLTIPSVEEILARMDPRPSDKGVLLIRKAYETAKEA
                     HKDQRRKSGEPYFIHPARVAWHLAGMINDPATIAAGLLHDVVEDCFLTNPAIKAQFGE
                     EVAELVEGVTKISAINFTSETENQVGNLRKMILAMARDVRVLLIKLCDRLHNMQTLEH
                     LPPPRQRAVAQSTLDIYAPLANRLGMTRMRTLLEDLSMRYINPAEFHRLERKLAVRAE
                     RDQRIVEETRRLLSEHLERMGIPAAVHGRRKHIYSIHMKMKRQGLRFEEVRDILAIRL
                     ITDTIAECYEILGIVHSLWKPITGFFKDYIATPKENGYRSIHTTVIGVENEVTEIQIR
                     THEMHQIAEEGIAAHWKYKEVGGSTNGSWGSEEKRLAWLRQLVEWLQDVHDPSEFMRD
                     LKRDVFEASVFCYTPKGDIIEMPRGSTALDLAFRIHSQLGYTCSGARINNRMASIRSE
                     LQTGDVVEIITSKNAHPTPDWLQIARTGRARNKIRHWLKESQHDLFLDRGRRMVLELA
                     RQRIGGAFEEARIDEILQPHLGSFNVAGLEDLFVEVGCGTIKVNSVVARLEQVLRPVE
                     PRRPPKAQRRPGKKKDVVLVEGMAGAVVRMARCCQPLPGEPIVGFVTQGRGISIHRED
                     CAAFEHIRARLGGAEHRVVHVEWGDASRALQKAAIRLVCQDRKGLLSDISTAVTQLNV
                     SIVGSNSSSNIRDNRAIIKLVVMIEDAEQLNRILTRLATVPGVLSLSRVVHDR"
     gene            complement(16171..16707)
                     /gene="def"
                     /locus_tag="PLS90_01875"
     CDS             complement(16171..16707)
                     /gene="def"
                     /locus_tag="PLS90_01875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPK01181.1"
                     /translation="MPLLPIRIYDDPILRETARPIEQVTDRLRALARDMAETMYEARG
                     IGLAANQIGELERIIVVDVDWASRNGRRALPRNPLTLINPEILDDSVEDDVCSEGCLS
                     IPGVEGDVWRPVRIRYRYTDLEGRTHEDEAEGLRARCIQHEIDHLNGVLFIDRMTPEE
                     RARLAGALAQIRRKRSEA"
     gene            complement(16739..17455)
                     /locus_tag="PLS90_01880"
     CDS             complement(16739..17455)
                     /locus_tag="PLS90_01880"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPK01182.1"
                     /translation="MTRPLIGINCDNFRDARGPITGVRDGYCEAVAAAGGLPVLIPPL
                     SGPAETDRLLDRLDGLVLTGGDDVRPERLGVAPAPSIVPMTPARDRSDFLLLEGLLRR
                     ATPTLAICLGFQELNVHLGGTLWQALELEGPPGRIAHRGSGAYHFTRHPVRTAADSRL
                     ARLWGDGEREVPSAHHQALRDLAPGLRAVAWAPDGLIEAVELEPHDGFLIGVQWHPEQ
                     APDDPAHRKLFEALIAAALK"
     gene            18083..18538
                     /locus_tag="PLS90_01885"
     CDS             18083..18538
                     /locus_tag="PLS90_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010342447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPK01183.1"
                     /translation="MASENTVRLHRVLRAAPERVYRAFVEPDALIRWMPPYGFTAQIE
                     QMDVRVGGTFKMSFTNFGTGKRESFGGRYLELKPGELLRYTDQFDNPDLPGEMQVTIT
                     LRPVACGTDLAIVQEGIPPVIPVEFCYLGWQESLLQLANLVEPEIPDGE"
     gene            18707..19150
                     /locus_tag="PLS90_01890"
     CDS             18707..19150
                     /locus_tag="PLS90_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPK01184.1"
                     /translation="MSSATIQQAQDEIIEQFSRLDGWEERYRKLIELGRQLPEIPEEY
                     QIERFRVKGCASQVFLHPEWRAGTVHFDATSDAMIVRGLIALLLRVYSDRRPDEILAT
                     PPDFIRKIGLSDHLSPTRANGLAAMVEQIRLYALAFKQLAERRAG"
     gene            19206..19817
                     /locus_tag="PLS90_01895"
     CDS             19206..19817
                     /locus_tag="PLS90_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01185.1"
                     /translation="MNQTPKPLPMRPWFWSPLLLIGVAALIIWRWASMAQTEREQLLA
                     TVREHQQASRAELAERWGALLDWTPPAAGEPAQPGAAAAIPDAEQALAVTPRHRFSDF
                     DAIYTAFVGDRAVAVLALTPVAGYFNGERLDLARHSDTLIGRKLTAAEREGLRVLIGL
                     LERRLDQFEAPTDRHGWTQNVLRTARQLESLQETGGGGATDGD"
     gene            20296..21651
                     /locus_tag="PLS90_01900"
     CDS             20296..21651
                     /locus_tag="PLS90_01900"
                     /inference="COORDINATES: protein motif:HMM:NF015980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPK01186.1"
                     /translation="MHAMMQRRPVRLLPALLFVFLLAAVETVRAQDPVDIGVVTGHRR
                     DTFPLYVPEFQVQPGSMQQLPENLFAQVVSNDLQLSGFFRPPDNVQFARETHQGDLQR
                     GEIQFAEWYRIGVLYLVRGEYTIVDGELRVEVRVYDTVAGSYIFGNLYPNNRPEQARA
                     LAHQISNDIFKRITGYDGVADTQILYVQQADSLGRTKQIGIMDADGYGMRTLTPGGEL
                     TATPAWGARGTEIYYTTYRDFNPDLEGMILRTQQRWWVSRRNGFNLSPAWNEALQLIA
                     LTLTKDGNSEIYTIDREGRNPRRLTNNPAIDSSPCWSRDNSQLVFTSDRTGSPQIYKM
                     NADGSSQQRLTYQGRYNDAAVWSPAGPERIAYASRVSDGTFQIFTCNPDGSDVRQLTS
                     GGSNNEDPTWSPNGLLLAFTSDRGGRKQIYIMFAADGSNVHRLTNGSPCHSPAWSPMK
                     H"
     gene            21732..22409
                     /locus_tag="PLS90_01905"
     CDS             21732..22409
                     /locus_tag="PLS90_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK01187.1"
                     /translation="MAGLVLICALGGCTPARSRLGAIHQYSTLDGTAEEEATEPERFD
                     AGPGADAATSPTLEPLGEEMRPGSPEHWGREARLAVGREFDPLYFEPGTLELTPAAAR
                     QVIDYGQWLTANPHVWITLAGHAGRQETVEFAYNLGMARALLVRERLISLGVDARRLF
                     VLSYGSEWPAVEAETREAEQLNARVEVTGFVAPLAQPAPPPESIAPGAAPAVQEAPPP
                     LRGHDIP"
     gene            22547..23446
                     /locus_tag="PLS90_01910"
     CDS             22547..23446
                     /locus_tag="PLS90_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HPK01188.1"
                     /translation="MASPTEAPSLWRRIRLTLGMIRFSHSIFALPFALLSAFLAAGGW
                     PARWTLGWIVVACVFARSAAMAFNRLHDERFDRQNPRTRHWALPAGLLSRRFVWCFLV
                     FCAAGFIFSAAMLNPLAFILSPAALAILLGYSTAKRWTAGSHFILGLALGIAPIGAWI
                     GVRGEWGWPPLLLGLGVLFWVAGFDMIYSCQDEAFDRRVGLRSLPARVGRARALALSA
                     LSHALALACFLLLTGLPGLGWGYLLALCVAAALLVYEQSIVSPADLSRLNMAFFTLNG
                     WVSLLVFLGGFCDLVLLGPGPIG"
     gene            23453..24187
                     /locus_tag="PLS90_01915"
     CDS             23453..24187
                     /locus_tag="PLS90_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPK01189.1"
                     /translation="MARRIHIGTSGWQYQHWKARFYPADLPQKKWLEYYAARIDTVEI
                     NSSFYAPPSETTVRNWRARTPAEFVFTWKAGRVLTHRKKLKDAGDMVPDVLARAEELG
                     DKLGPVLIQLPPRWKVNPDRLREFFAAWPGGRRVTLEFRDPSWFTGEVLEILTENRAA
                     LCLYEIGGQTAPEEVTADFVYVRLHGPGRAYQGNYSDAQLGKWADFFRRQRGRKREVF
                     CYFDNDDSAYAVHNALRLREMLHLSH"
     gene            24367..26241
                     /locus_tag="PLS90_01920"
     CDS             24367..26241
                     /locus_tag="PLS90_01920"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HPK01190.1"
                     /translation="MDDMAKATGDEALAHSASVYSNGMIELHAAGNEVVAFQLVFQAD
                     RVERGLDLRASDLRGPATLAADDVFEFFLAHYLSTTDGSYGWFPPGTAGVLPWEGKLW
                     PDPLIPFKDPWTTPAAAVAAPFAIDPARQRNQAVWVDVFIPKGTPAGWYEGQIDCLRA
                     GRPFREIPIRIEVFPFDLPDESHADAWGELYRETGVMFDSGVKFKEHPEADWPIYRRY
                     QQLAHAHRLLATQRQGMGPVPRTPAGRPADRPDDQWGEDWSLFTPYVAPVLDGTLFTE
                     SAGYRGPRAGAGPTFFPAPFVETFYGADSLRAHLEKHKGRIDPELLETWTRNAAAFWR
                     EVQRQGWEDRRFFAYLLDEVDSARDGEGGAKIPMDEITRFHAAMRSIQAALDAGTDGR
                     RRIHLLWTSHADATRWLGTPADLTPFISWWAPNGHALQTDFFPRAAATPGHTVWFYHS
                     GHPAVGNHGINQLGIDLRLWGLLCARYAIDGFFVWSLMSFPERWDAEGFIPYNHPGYR
                     EGENRWGNGTLLYPGSRLTAVGAARAIEGPIPSYRLKALRRGLQDAEYAWLARRAGRG
                     EATDGLLRGLIPAAFSEAPRPRWWRPRPHGLWSRRPEDYAAARLQLARLIQEGADPAD
                     "
     gene            complement(26274..27578)
                     /locus_tag="PLS90_01925"
     CDS             complement(26274..27578)
                     /locus_tag="PLS90_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HPK01191.1"
                     /translation="MVVEALAGNVRGTYMRYDTAVILKRLFLVERMLVVSQAGWLPGI
                     ADFNMKTALPKMLWEDALTADALRERVFELRFPNRMLEIGDDAPLVNLLRFAQAAPSA
                     PAFLMALAKVFKPALRKAFNSYLELADDLGDGPTYRFMRLATEELRGHIEQIEASLAR
                     VFETDPEEGRRSADWVRRLQQLLAEIGGIGLDPPRAGSDLPALERPFTPAETPARDAQ
                     FHNLRFYWPDIIDPTFPYGEGLRLQLRSAVSHFNEVWAVENAGFILHTYADDLGWEFI
                     RDAARWVYDESRHVRMGYERLKTWGFEDRELPLGTYIYDSCRGQDPLYRIGMLFYFES
                     KNIGKKMERKKSFELLEDRMSQHDMDFDWADESIHTSYGKTWLSALLKLRGENPEDYT
                     AIKTECERLVAAVLETAEERERVEVRRVAEHMIERAQQLARR"
     gene            complement(27721..29151)
                     /locus_tag="PLS90_01930"
     CDS             complement(27721..29151)
                     /locus_tag="PLS90_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01192.1"
                     /translation="MAVSTKQGLLESAYRSGEFPVQSRPEERQAAEARQKAYPYRFPR
                     DRRQLGGKFSVQQNAEILSRFFYFERRLAQALGSWTLSIPDFEVKLETGRHIFYHADA
                     AHQLRERLTEQEMTLPKVDAYRNEEIDRFIEEMLSAADAPELLVGVHQVAGRALDLAY
                     AHHCDLTDPITDAPTIRILRRIRMDYEPMLEWAEQAIEAYIEGGVDESRLSVWRWHLQ
                     RLLASIGGVTGTAERAEQPSPLRSAARPYERGTVPLRDSRFTTFRHTGDYDAADGEPR
                     FERGSYESFRLHFIRTQRDEVDAIEAFGTFLWDIRFKDFDAEYALARITWDEARHTEI
                     GHRTMQAMGYNPFELPNRLTSSTCRGEMTGDNAAFAMAEINLFGEVSILKTINGLHDS
                     AKLANDILLAHDSDYIRADERTHVRKGRHILSVMTDLGSRDLERRTRELFTECLVSLG
                     AVTAETLSLMQDGILSREEIEQLVGE"
     gene            complement(29439..30686)
                     /locus_tag="PLS90_01935"
     CDS             complement(29439..30686)
                     /locus_tag="PLS90_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HPK01193.1"
                     /translation="MNSPHACPDPQLDALLVSDEIMEDPYPIYERLREEQPVFWSDAW
                     QAWVISRYDDVEASLKDKDNLSNENRQALLFSALTEAERAEVAPLRHYYAQKDVIGSD
                     PPDHSRMRALVQKAFTPKTVAGLGPRIEALAHELLDHALQSRRFDFIAEVAHPLPVIL
                     IAEMMGAPVKDRHLFKRWSADILAFQGTGVTTADAARISQASLLEMFDYMNELIDSRR
                     VEPRDDLITALANAEEEGQRFSRDELLATCNTILTAGHETTTNLLGNLLHLLLRHRDQ
                     WTALVRDPNLITPAIEEALRYDAPKQRNFRRVKRAHEFLGVPFAEDEMVFQLIGSAQR
                     DPRHYDQPDVFNIQRGKIEHLAFGAGIHFCLGAPLARLEARKVLEIMLARCPDVELEP
                     ETVKWQPRVQFRGPGEMWIRRGA"
     gene            complement(30733..32520)
                     /locus_tag="PLS90_01940"
     CDS             complement(30733..32520)
                     /locus_tag="PLS90_01940"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPK01194.1"
                     /translation="MMTGRKNQIDAMRHGLRLLCAGALLLAVAMPCRAAEENAPMSKL
                     TPEQAAMWDISPLRETPLDVEIYSSKVVDGCQVQDVFFTSQSGPDGPNRVYLGVAFPA
                     DREGPFPVLLDLHGGGGVGTSARALSFAKEFDVFAMAMDWGGKSESDPNPLRTVWRYP
                     KPGTETTTGTRSSAYRTAMGMRRALDFAATQPMVDMSKVVVYGGSWGSYYTTLLAGID
                     ERITDAVALTGAGGWDDSRSIIADGINLLPPALRDEWLRQFDPISYADNVRADVSLVA
                     HANDSFFWFGGVMRHFDRFPGPKRVIITPNSDHGIGTFPEPIATWPFTYSILQHHIEG
                     VPFPEVTRESVREVAPDVFEWETAGPQVEGAHLYFSPGDVNWRSTYWVELPARREGQA
                     WRAELPEHLRGLAGNYFVTVCASDNRCVSSPVMWREGKNPQRDWVNLWPINPLWDVSA
                     GIRAWRQSIYKKETVIEPAGPEGLRVTPGSNDLFLVRTDSTVLLGPRAGQFAGLEIVV
                     NGEGQAGELRVQLLDDDKTRNIPNIYEARVPYGEGETTLVIPWVDFEAPECVREGGGR
                     WPFESLALTGVRPGRKPLTFVSIRLADKE"
     gene            complement(32517..>33267)
                     /locus_tag="PLS90_01945"
     CDS             complement(32517..>33267)
                     /locus_tag="PLS90_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01195.1"
                     /translation="VFIGEYGLPLPPVPQRPHRNRLGLKAVASWGSPINLFWSTYTQL
                     ENDNSALFSLEGEKTEDYYVLADYVAKMHFLRNATRVWLERNPTDQEASWLALDYDRI
                     APHDILRRILDSLEYRFTVTDEEFIESIFASCGMTGSGALTEDLVTSLREGRLTRFEA
                     LCRILDSDEFAGAMGEEEFDAWLAMHLLSDTVEFPDGAPTRSERYLSALDHEAFRDRN
                     IAAAQLNHVTPELRRMYQPEFRASGEAEDAS"
BASE COUNT         5214 a        10631 c        11498 g         5824 t
ORIGIN      
        1 tgttgccgtg atccaggtgc agcgcaacgg ggatgtgggg gtattcctcg acggccgcct
       61 ccatcaactt cttcaggtag atcatcttgc tgtatttcag ggcgccgcgg ctggcctgaa
      121 tgatcacggg gctgtgcgtt tcgttggccg cctccatgat cgcttgaatc tgttccatat
      181 tgttgacgtt gaacgcgccg acgccatagc cgcccttggc ggcttcatcc agaatctgac
      241 gcatcgggac aagacccatg agcgtatctc cttgttggca agatccattc gggtgtgggc
      301 gggggatcgg gcgtcggacg gcttgcggac cgcgcgcttc gttccagacc agccgggttg
      361 gccccaacgg actatcttag catacgcccg gggcgaatca accgcaaagg agcgcgacgt
      421 atgaaaggca acgtatgaaa ggcgatgtgg cgctcgccgc atgttggagc aagttcgtgt
      481 ttcagaaggg agacacggga cgcaagggac gaaagggaca tcaggacggg actggcctgt
      541 tgacggatcc ggcgaggacc tggtcgttcg attcggttgt ttacggctgc catgttcatg
      601 catcgttgtg aaccgcaggt tcatgtgtaa ctcgtaaaga tctccggccg cccctgccag
      661 gggcttggat cgtgggggcg gggacgcagg gttccggcct gcggccgtca ccctgcgctt
      721 tactccggcc gtccgctccg cggactgaac cgatgaacaa gtcggaagcg cctgaatgga
      781 agttggaacc ctgaagcaga tcgcgatttg gggggataac aagcagtcaa gttccggcgc
      841 cggtctcgcg actatttcat tcccagcttc cgctgcatgc cggccgtgat tgactggcgg
      901 acgtcgaaaa agccgtccca cgggtcctgc ttcagtgcgt tcagccggtc gcgcaggttc
      961 tcgacggtgt actggctggg cgaaaggtcc gcggtcagtt cgtcccagcg gatcggtgcg
     1021 gagacgggcg cgccggggcg cgcgcgcgtc gagtaggccg ccacgctggt ggctccgcgc
     1081 gcgttgcgca ggtagtcgat gaagatccgc cccttgcgcc gggccttgct cattgtggcg
     1141 atgtagtgcg ccgggtcgtc cgccgtgatc gactcggcca gcgcgcggca gaacgccttg
     1201 acgtcgtccc acgacgtgcg ccgctcaatc ggcacgcaga cgtgcagccc cttccccccc
     1261 gacgtcttga ggaagctgcg cagcccgagt tcctccagcc ggtcgtgcat cagccgcgcc
     1321 cccgtcacga ccgcctccca ccccaccgcc ggatcgggat ccagatcgaa aacgaggaga
     1381 tcggggcggt cgacgcggtc ggcgcgcgcg ccccacgggt ggatctccag cacgcccatc
     1441 tgcaccagcg cgaccaatcc ctccagcccc gtaatcatca ggtactgctt cgggccatcc
     1501 ttctcttgca cttccacgcc cgagacagcc ggatggaccg tgtcgttgac atgcttctgg
     1561 tagaagcaat gcttctggcg gccggcgggg cagcgcacga tgctcagcgg ccggttgacg
     1621 acatacggca gcacccagtc ggcgatgtcg acgtagaact gcgcgatctg ccgcttggtc
     1681 aacccctgct cgggatacag cacgcgctcg gggttggtca gagtgacgcc ggcgatcatg
     1741 tcttcatcgc tctccgtggc gcggttgcgg cgcgccggtg cgccggcgcg cgcgcggccg
     1801 ttgtccgccg ccgtcgcggc gggccgttcg cggcgaacat ccttcggctg tttgtcctcg
     1861 cgcagaccct ggaatgaggg atggcgcagg atgccctcgt cggtccattc ggtgaacgcc
     1921 acttcggcca ccagctcggg ggccacgtaa tgcacgccgc gcgcatcggc ccccttcggg
     1981 ccgcggtcga acgggctctg cctgcgttcg cgccggcgca ggtcctcatg catccgtttc
     2041 agcacccgga cgctgaaacc cgtgccgacc cggccgcaat agatcagcct gccgccctca
     2101 tcgtagtaac ccagcacgag cgcgccgaag cccgcacgcg agccgcccgg atccgtatag
     2161 ccggcgatga cgaactcctg gcgcctgccg cacttgagct tgacccacga tctggtccgc
     2221 cgttcagcgt acggggcatc ggccttcttg ctgatgatgc cctccagacc gaggcggcag
     2281 gcgttgtcga ggacttcgtc gccgccgccg cgcacatgat cgctgaagcg gacgcggccc
     2341 ttccgcccgg cgcggaccag cagccgccgc acgagctcct tgcgctcgag ttgccccgcg
     2401 cgccgcagat cgtatccatt caggtgcagc aggtcgaaga cgtaatagca cagctcggcc
     2461 tgtttcccct ggcgggtaaa gttctgcagt tgctgaaagc tgctgacgcc cttttcatcg
     2521 aggacgacca cttcgccgtc gagcagcgcg tcgtcgacct cgagctcccg cagcgccgtc
     2581 gcaatcgctt cgaaccgggc ggtccagtcc ttcccgttgc gcgagaggag ccgcgcgcga
     2641 ccgtgctcga ggcgcgccag gatgcggtag ccgtcgtact tgatttcgtg cagccagcca
     2701 tcgccgcgcg gcggctgcgc cacgagcgtc gttagttggg gccgcgggtc ggccgggaga
     2761 gggccgcggc gggccccctt gacgcgcgcc ggatcgggcg gctcggcttt tttcgttcgc
     2821 ggcgcggcgt cggcggcacg gccggtggtc tgcttgcgca gttccttctg cttgcggtcg
     2881 ggatcggccg tctcgcgatt ggaccgccac tggcgatcct tcgccgcggc gacctggttg
     2941 atgtcgcgcc cggtctccac gctcgccggc tcgtgatcgg tgatgcggcc ggccttgccg
     3001 cgccgcgcgg cggcgtcctg ttccttcacc agcagccagt tcctgccgtc atcgccgcgc
     3061 tcgccgctca tccgcacgag catccagccg ccgcgcagct tctcgccctc caggtcgaat
     3121 ttcaaatgcc cctcgcgata tgccttgcgc gggtccttgt cgcgcggatg ccagcggccg
     3181 cgatcccaca gcaggaccgt gcccgcgccg tagccctcgg ggatgacccc ctcgaagtcg
     3241 ccgtagtcga cgggatgatc ctcgacgtgc acggccagcc gcttgtcggc cgggtccagg
     3301 ctggggccct tgggaacggc ccagcttttc aagacgccgt ccagctccaa gcggaaatcg
     3361 tagtgcaggc ggctggcgtc gtgtttctgg acgacgaatc gatcgcccgt cgccgtcctg
     3421 gccgtgcggc cgcgcggctc ggtcgtcttc ttgaagtcgc gtttgcgatg gtaatccttg
     3481 agcgacatgc gcgcctcggt tcccggcccg ggttatccgg ccgcgcgttt gcgggccgct
     3541 tttttcgcgg gggccttccg ggccgacttg ccggcctgct ccttcttcgc gccccccttc
     3601 atgctgcgct gcagcagctc catcatgtcg accacttcgg cctgctcgtc cttctcctcc
     3661 ggttcctcct cgagcggctc ggcccccttg cccgatctgg cctttttttc aatgaacgcc
     3721 atcagtttgt cgcgatagtc atcgtgatat tccgcggggt cccattccgc cgacatcgcc
     3781 tcgaccagtt gctcggccag tttcagttcc ttgtccgaga ccttgtattg cttcaggccg
     3841 ccggtgggca ggtcgaattc atccaagtcg cgcagttcct gctggaagcg cagcaggtcc
     3901 agcaccagcc cgtcgccctg cggcaccagc gccgccaaat gctgacgcgt gcggatcacg
     3961 accttggcga tgccgacctt gcccgtccgc ttgagtgttt cgcgcagcag gacgtaaccc
     4021 ttctcgccct tcttgccggg gacaaggtaa tagggcttgt cgaagtaggg atactggatc
     4081 gcttccagat cgacgaagct ttcgatttcg accgcctgtg aattttcgac cgccgccttt
     4141 tgaaaatctt cgtccgtcag cagcacgtaa tcgtcgccgt catactcgaa agccttcacg
     4201 atctcgctcc acgggacttc ctcgcccgtg acctcgttga cgcgctcata gcggacgcgg
     4261 gcgcggttgc ggctgtcgac caggttgaag tgcagattat tgcgcttttc agccgaatag
     4321 agcgtgaccg ggatattgac caggccgaag gtgatgttgc ccttccagat gggacgcgcc
     4381 atggcactcg ccttccgtca atcgcggatc aggcggcgag tcgtccgaac caggcggaaa
     4441 tcaacggggc aacgggaagc gaaccgcaca actcgccacg tgccctgggt gcagagcaag
     4501 cactgttcca gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgaggccg
     4621 cgccgctgtt ccgcggcaac cccgaggagc gtcaccggat cgtcgccgcg atcggcgtcg
     4681 tggcggcgat gtcgcccgcg gccaaagccg cgcttgccgc ccgcaccgcc gatacgctcg
     4741 cgctggccgg cgccatgccc gggacggcgg tcgccgcggg cggaggcgcc gccgggatcg
     4801 gcctgggcgc ggggtcgctc gccctgctgc tcctgctggc cgtcggcggc ggggtcttct
     4861 ggatgcggtc gaacgcggcg cccgccgcgc cggcggcgat cgtcgagctc tgggggtcgg
     4921 acacgccggc gacgcgcggc gacaatgccc ccggcccggg cggcccgcgt tcacgcgcga
     4981 cgccggcggc tataccgatt tcgcccacct gccgccgcag cccaatctgc tcaacccgac
     5041 gcgcgacctg gccggccggc cggcgctcgt gatcgacggc gtcgatcacc acggcccgct
     5101 tgccgagcgt ccgctgcgcg gccagtcggg ccgggtctgg ggcggctact gcagcgcccg
     5161 ctcgaccgcc gcgtcgcgcg gcaacagcca tcacctggcg attttccatg gcgcgctgct
     5221 ggcggccgag cgcgatccca tgttgaaccg cgggttcctg gatttcgtct cgcacagccg
     5281 gccggccgcc gccgacttcc cctcgcccta taccgccgac accggctcct atgtctatac
     5341 cgttttctct tccgatcccg ccgggccggc gatcgtcgac gtgcgcgccg gacggctcgt
     5401 cgcgcgctgg aacttccggg ccggcgatcc gcgcttccgc attctgcttc agttggacgg
     5461 ttcggtctgg atccgcaacg acgcggatct gccggcgctc gaccggcccg gcggcgccgg
     5521 ggacggtccg tcgttcaacc tggccgagct ggactgggaa cgcgtgtcgg ccgacgccga
     5581 gcaaaacatg aatcggctcg atgacggggg ggaagtgccg ctgggcccgg gcgcagcggt
     5641 cgcagcggat ctggcccggg tcaccggcat gggggtcgtt ctctcgcggc tggccgacgg
     5701 cctgtcgtcc gcgggcgtgc cgaccagcgt gcaactgatg gagctgcggc tggaagggct
     5761 ggcggcccag taagcgaggg tctcactgga aaattcgtcc gctattcgcg ggatcgctgg
     5821 gatcggttct gccgcccccc tacgggactc tgtcgcgatg ggtatcccgg ttcccgccac
     5881 tgacggtgcg ggctgaaatc tgccgtccct gacgggactg ccctgctgtt agatccggca
     5941 aggcctggat cgttcagccc cattttcatc cctcgttgtg aaccgcaggt tcctgtgcca
     6001 ctcataagcc taatcgcctc aatcttaccg ggaggctggc gggcgatagc gagaccgatt
     6061 gcgaaaccga tcccgctcgt gcccgggcgg cagggcggga acaaatgacc gccgcgggcg
     6121 gtcctgtcag gaaagaatgg acggtcggcg tccgttctgc gaaattgctg aggcgcaacg
     6181 ctcgcacggg cggaaccgcc gaaggagccc tgaacgtgaa ccgaacccgc tggttacttt
     6241 gcattttgct ggccggatgg ttgagcctga gtctgcccga ttcgctgccg ggtcagcagc
     6301 ccaatcgcga tccgggcgag gatctggaac cggaagaggc ggcgctgccc gattttggcg
     6361 aggcggggct gagcgagccg attccgatcg atgaggccgg cgcgccgatc gtcgagccga
     6421 cggagggcgc cgaaatcacc agcgcgacgc tggaactgct gcgcgagctg gagacctttc
     6481 accgcgagat cgacacgatc cacgtgacgt tcgaccaggt gcggatcgat ccgatcttcg
     6541 aggaccggat cgaatcgcaa ggtgaactgt ggtttgacaa gcccgaccgg ttccgcgcga
     6601 actacgccga cccgcggccg atgaccgtca tggtcgtcga cgatacctac tatcagtatg
     6661 tcccggaact ggagcaggtc gagtatctga cgttcgaaag cgaagccgac cgcaacgcct
     6721 cgctgcactg gctgctgatc ggattcggcc tgaaggtgga cgagctggcc cagcgctacg
     6781 cgatccggtc ctcggcggac gaccccgcgc tgcggaccga actgggcctg gaggcggcgg
     6841 aaagcaaggc gctgttcgcc gtccggccgc gcccggcgtt cgaggaggca accccgttcc
     6901 gcgagctcaa ggtaactatt gacaaagacc ggatgttgcc cgaaaaggtc tggtacgttg
     6961 actacaacga cgcggagatg aacgtgacga tgcgcgagat cgaactggac caacggctgg
     7021 acgaggacct gttcgacgtg cgcaaggcga ttccgcgcgg gacgacccat atcgacatgc
     7081 ggagcatgca atgagcccct tccctgccgg cccgcggcgc atcgcgctgt ggctcctcgc
     7141 gcttggtctg ctgccggtgg cggggtgcga gcgcgtcgcg ccggtgcgga cgctgccctc
     7201 ctgggtgcgc gggatctacg tgccgatgtt cgagaacgag acgttcgagc ccgggatcga
     7261 acagctggcg acgaagatga cccaggagga gtttctgcgc gacgggcggc tggacgtcgt
     7321 gcccgaacgc gacgcggacc tcgtgctcaa ggcgaagatc accgaatggc tgagcaaggc
     7381 gggcggctcg tcgggcgaca agatcaccga ggatgaaaaa atcacgatgg tggtcaacgt
     7441 cgggctttac gagccgttcg aggatgaacc gctggccgtg ctgaaaccga tccggatctc
     7501 gagcaagttc aacatcgacg tgcggtcgag cgattttgaa cccgagccgg accggcgccg
     7561 cgcgctgttc caggcgctgg ggcggcggat cgttttcgac acgatcaccg gatttccgcc
     7621 gatggtgcgc gacctgccgg ccggcgtgac gctgccgtcg atccccaatc ccgaagacat
     7681 cgaaccggat cagtcgctgc tgccgcggcg cggacccgat ttcgactgag ccggggcgtt
     7741 tgcgtggggg gagcctccgc tggatgcccc ccggcaatgc cggcaaacgc acggtggcgt
     7801 cgggtgcgct gccgccgggt tcggttcgca ttcgggactt cggcggcgtc gctgccgtca
     7861 tctggccttt cccctttcct ttttgatggg tccttggaaa gatcgtcccg gccgccggcc
     7921 cgcgccgatc gggcgggtcg cgccgatcgc gcatgaccac cccgtccccc agccccgctc
     7981 cttccactcc gccgccggcg gcgcaatccc cggcggcgga agcgctcgtt gcgcatgatc
     8041 tgtggcatca gttccagccc aactggtggg tgctgcgggg cgtggagctg acgtttcgag
     8101 cgggggaact ggtggggctg gtcgggccga acggcacagg caagacgacg ctgatccggc
     8161 tgctggcggg tcaggccgcg cccagccgcg gccaggttac gctgggcggg cgcccgctca
     8221 gcaagatcgg ccggctggag ctggcccgcc agatcggcta cctgccgcag aacgtccggt
     8281 cgtcgttccg cctgacggcc gaggaggtgg tggcgcaagg gcgctatccg catcaacgcg
     8341 ggctggggct gctcgatgcg cgggatcatg cggtgatccg ccgcgtgatg gacgagacgc
     8401 ggacgctggc cttcgcgcgg cggcagatcg aggacctgag cggcggcgag cgccagcgcg
     8461 tgctgctggc gtcggtgctg gcccaggagg ccccgttcct gatgctggac gagccgacgt
     8521 cgatgctcga catccatcac caggtcgagg tcttccagca catccgccgg ctggcgcggc
     8581 gcgggctggg cgtgctggtc atcacgcacg acctgaacct ggcggcgcac tactgcgacc
     8641 ggctggtgat gatgttcgag gggaaagtcg aggcggaggg cgcgcccgaa gcggtgatgc
     8701 gcgaagccgt gctgcgcaag gtttacgaaa cggagctgat cgtcgaccgc aacccggtga
     8761 cgggatcgcc gatggtcgtg ctgctgggca accgcgagga ggagcgggag gatcgccgat
     8821 ggccctgctg acggcgcgcc ggtttctgct ggtgctggcc ggctatgcgc tcttcgcgct
     8881 ggcggcgatg ctggcggcgc cgatgttcgg ggccgagccg gtcaacgtgc cgcgcgcgct
     8941 gagccagtgg tgggagacgc gcggggccgc ggtccggccg tatgaagtcg acatcctgct
     9001 gcatctgcgg ctgccgcgga tcctgctggc gtttctgacg gggggggcgc tcgccgtcac
     9061 cggcgcggtg tttcaggcgt tgctgcgcaa tccgctggcg acgccctaca cgctcggggt
     9121 ggccagcggc gggtcgttcg gcgcggtgct ggcgttcttc ctgccggcct acctgccgtg
     9181 gatcgcgttc gagctgggac cgttcaatta tgtgcaatgc ctgtcgttcg ccggctcgct
     9241 gggcgcgatg gcgctgattt acggcctggc ccgcacgggg gggcggatct cgacgatgga
     9301 actgctgctg gcgggggtga cgctggggat gatcttctcg tcgctgattc tggccgtgcg
     9361 ctacttcgcc tcgccgcata tcgtcgtcaa catggaccgc tggctgatgg ggggcctgag
     9421 cgtgacgggc tacggcgaag tgcgctcggt gctgccgttc ctggcggtcg ggctgggcgt
     9481 gcaactgggg ctggcgcgcg cgctcgacca gttggggctg ggggaggaac tggcgcagag
     9541 ccggggggtg aacgtcgccc ggctgcaggc gctggtgttt gtcttcggct cgctggcggt
     9601 ggcgtcggtc gtggcggtgg cggggctgat cggcttcgtc ggcctgattg tgccgcacac
     9661 ggcgcggcgg ctgacggggc ccgatcaccg cctgctgctg ccggcgtcgc tgctggccgg
     9721 gggcgggttc ctcgtgctgt gcgatacggt ggcgcggacg atcatcgcgc cgaccgaact
     9781 gccggtgggg atcatcacgt cgctgctggg cgggccgttc tttatttatc tgctcgtccg
     9841 cagccggcgc ggcggcagaa tgtggggcgt ggactgaggg ggcgttcggg gcttttgtgg
     9901 gagaattggt gcttcagatc agaagagaca taagagacat aagggacatc agggacgcaa
     9961 gggacagaaa ggacgcatgc agggccaagc tggaaggatg gccataggtt cccatctcga
    10021 acagggtcgg agttgtgaag gccgccacga caccgatgcc tgcccatgcc ggcggggcga
    10081 cacgccggat aatgccgtag agcagaccga cgccgacgcc catcgcaagc cacgatagcc
    10141 ggttcattaa cgggatgagc ccgttcagat tttccgcgac agggcctggc cagttcagaa
    10201 atagcagata gtacaacggc tggctgaccg gaaagtacag catcggttcg aggagtcccg
    10261 ttccctgcca tgtcgcctgc acgaccccgg cgcccatgac gtagcagtca tgctcggcga
    10321 agccgtcgtg gtagatccag gggaaggtcg cgatcaggta ggcggcggag aggccgaggg
    10381 cgatcagcag gtcaagttgg cgggaggttg gtttccaggg ttccatctag catcccgcgg
    10441 gtgaggattc ggttccggtc cttgcgtccc gtttgcttcg ccggtcagtt gacgacgttg
    10501 atcggcgcgc cgcgcaggaa ggcggcgatg ttctccgcgg tcgtccgcat caggcggcgg
    10561 cgggcctcga gcgacgccca cgccatgtgc ggcgtgatga ccaggttggg cgcacgcagc
    10621 agcgggttat ccggggcgat cggctcggtc gacacgacat cgagcgcggc gccggcgagc
    10681 cgccccgccg caagcgccgc cgccaggtcg gcctcgttga tcaggccgcc gcgcgcggtg
    10741 ttgaccaggt aggcgcccgg cttcatccgg gcgagcagcg cgcggtcgac catgccggtg
    10801 gtggcggggg tctgcgggca atgcagcgtg acaaagtcgg ccaggctgaa gagctccgcc
    10861 agcgcgacgc ggcggaccgg gtaggcgacc tccgggccgg gcgaggggct gcaggccagg
    10921 acgcgcaggc cgaaggcgtg ggccagctcg ccgacgcgcc ggccgatccg gccgaagccg
    10981 acgatgccga tggtcttgcc cgccagttcg agctgcgggg tcagccagaa gcagaagtgc
    11041 ggcgaccggc tccattcgcc ggcatggacg gcggcgtcgt ggcgcgcgat ctggtggcaa
    11101 tggttcagga tcagcgcgag gacgaactgc gccaccgagt cggtgccgta gaccggcacg
    11161 ttgcagacgg ggatgccgcg gcgccgggcg gccgccccgt cgacgacgtt gtagccggtg
    11221 gcggtgacgc agatcaggcg cagggcgtcc agttgcgcga tcgtctcgtc gctgagcggg
    11281 gccttgttgg tgaggatgat ctgggcggcg cgcgcgcgct cgacgatctg ctccggcggg
    11341 gtgtggtcgt ggatttcgag gtcgcccagc gcggcgaccg ggtcccacgg gttgtcgccc
    11401 gggttgagcg tgtagccatc gagcaccagg atccgcatgc cgttcacctg cgagggattg
    11461 gtcatggaag tcttagcata tcccaccggc cgtgcctagc cgtctggccg gggcggcatg
    11521 gcaaattttg atgtggaatc cttgctatta ataacggtat tattgaaaaa atgaattggc
    11581 agcatatcct ggagggtagc catggtctga attcggcgtc gccggtccca gatcatcccg
    11641 gaggaatgcg catggatcgt gacgcgttcg cagcccgtct ggccgccagg ctcaaaaagg
    11701 ttcggggcaa gcccatcgat caaatccccc agggggatta ctgctacgag tcccatatcc
    11761 ccggtgggga agataacgat ccggaattgg ctgcctgtct gaaggacggc gaaacgttga
    11821 atgccgcgta ttttgttctc tgggtcgaga agcataagga gcggaactgc ccctattggg
    11881 aagcgacgga ctacgggacg gtcaagtgcc actatctgga agaggaagcg cttggattgg
    11941 atgaagaaga ttatgaaaag gctctggccc aatttggctc ggaggaagag ctggcccgga
    12001 attgcaccga caatttctgt ctcgccgatg cgtgccgctc ttgccgactg gtcctggaag
    12061 cactggaagc ggagcgacgg cgggagtcct ccgaaaatta ggccctgggg atccattgaa
    12121 tcggttctgc cgtccctccg ggactcggtc gcgatgggga tcccggttcc caccactgag
    12181 gtagcgggct gaagtctgcc gtccctgacg ggactgcccg attgaaggat ccggcaaggc
    12241 ctgggccgtt cgaatcggcc gtttggcccc ccattttcag ccctcgttgt gaaccctcga
    12301 tttatgagca actcgtacca gcagcgccgc gtcaaggcgg gtcagaagaa accgggcgtg
    12361 cgaatctggc gcgccgtcgc acggacggcc gtcgcgttgg cgcatgcaat gccgcgtcgt
    12421 ccggaaacgc gatacgggcg agccgggaca tctcgggggt tgaccgggcg accgggttga
    12481 cgcacgctgg tacgcggtcc tggccgcgcg cgcccgtagc tcaactggat agagcaccgg
    12541 ccttctaagc cgggggttgc aggttcgagc cctgccgggc gcgccaggcg gaattcctgg
    12601 cattgcgcgt ccttacctta cgcttgcttc gcttccaccg tctccgagtc ccgttcaaaa
    12661 aagttcaaaa tccgagccgc ggagccggcc gcgattggat tggcgtggtt cccagcgagg
    12721 ggctgaaaga cctgcaccgg ctggttggag gcgacctcct cggcctgcgt cacggccgac
    12781 aggcgcggga tatactgcac tgattgcgcc ggatcgcgcg caggcggcct tcgccgcgac
    12841 ggcgcgtttt agtggacacg cttcgagacc aatgatccgc ttgaccccct gctggttgtg
    12901 ccattgaagt tgcacgtcgt cgattcagcc accccccccc gtggtggcgt ccgtcatgac
    12961 gctggtgatc gcgggcggcg gcacatggga taagcctgaa aaggtcgtga ttgaataatc
    13021 gccccgaggg gacagccgac cgggttggcc ttggcgagta ccccttcagc cgctgggacg
    13081 aattcaagta ctgctggccg gctgattgtt catgggggtg gagggcggga aacttggcgg
    13141 agtgggtatg taagccgagt tctgtgcgcg cggcgccggg ccgaagcgta ggcgctgcgc
    13201 gcggcgatca ttcctccagg cccgcgggtc gccccggggc tcaagcagcc tacccggagg
    13261 tcgagcgcgg cgggccgccg catccctccc tatttggcct tgcaccggat ggggtttacc
    13321 tcggccgccg tgtcgccacg gcgccggtgc gctcttaccg caccatttca cccttacctg
    13381 tgagcggcgg cgcgggggcg tcgccgttcc atcggcggtg tcgtttctgt ggcactttcc
    13441 gtcgggtcgc cccgccttgc cgttagcaag catcctgccc tgtggtgctc ggactttcct
    13501 cccgccggcg cgaaatcgcg ccggccggcg atcgccctac ccactccgcg ttgccatgct
    13561 acacccgttg gcctgttggt ggaaggcgat gttgcacggc cgggtgcgtg gacctcaaaa
    13621 aaaatcagcg gtcgtggacg acgcgactga gcgagaggac gcccggcacg gtcgccaggc
    13681 gcgtcaggat ccggttgagc tgttcggcat cctcgatcat cacgaccagc ttgatgatcg
    13741 cgcggttgtc gcgaatgttg gaggaggagt tgctgccgac gatgctgacg ttcaactgcg
    13801 tcacggcggt ggagatgtcg ctgagcagcc ccttgcggtc ctggcagacg aggcgaatgg
    13861 cggccttctg cagggcgcgg ctggcgtcgc cccattcgac atggacgacg cgatgctcgg
    13921 cgccgcccag ccgcgcgcgg atatgttcga aggcggcgca atcctcgcgg tggatcgaga
    13981 tgccgcgccc ctgggtgacg aagccgacga tcggctcgcc gggcagcggc tgacagcagc
    14041 gggccatgcg caccacggcg ccggccattc cctccaccag cacgacgtcc ttcttcttgc
    14101 cgggtcggcg ctgggccttg gggggccggc gcggttcgac cggccgcagc acctgctcga
    14161 ggcgcgccac gacgctgttg accttgatcg tgccgcagcc aacctcgacg aacaggtcct
    14221 cgaggccggc gacgttgaac gagccgaggt ggggctgcag gatttcgtcg atccgggctt
    14281 cctcgaaggc gccgccgatg cgctggcgcg ccagctcgag caccatccgc cgtccgcggt
    14341 ccaggaagag gtcgtgctgg ctctccttga gccagtggcg gatcttgttg cgggcgcggc
    14401 cggtccgcgc gatctgcagc cagtcggggg tcggatgggc gttcttgctg gtgatgatct
    14461 cgacgacgtc gcccgtctgc agctcgctgc ggatcgacgc catccggttg ttgatgcgcg
    14521 cgccggagca ggtgtagccc agttgcgagt ggatccggaa ggcgaggtcg agcgcggtgg
    14581 agccgcgcgg catttcgatg atgtcgccct tgggggtgta gcagaagacg gaggcctcga
    14641 agacgtcgcg cttcagatcg cgcatgaatt cggagggatc gtggacgtcc tgcagccatt
    14701 ccacgagctg gcgcagccag gccaggcgtt tctcctcgct cccccagctc ccgttcgtcg
    14761 agccgccgac ttccttgtat ttccagtgag cggcgatccc ctcctcggcg atctggtgca
    14821 tctcgtgggt gcggatctgg atttcggtga cttcgttttc cacgccgatc acggtcgtgt
    14881 ggatcgagcg gtagccgttc tccttcgggg tggcgatgta atccttgaag aacccggtga
    14941 tcggtttcca gagcgagtgg acgatgccga ggatttcata gcattcggcg atggtgtcgg
    15001 tgatcaggcg gatggcgagg atgtcgcgga cctcctcgaa gcgcagcccc tggcgcttca
    15061 tcttcatgtg gatgctgtag atgtgcttgc gccggccgtg gacggcggcg gggatgccca
    15121 tccgctcgag gtgttcgctc agcagacggc gggtctcctc gacgatgcgc tggtcgcgct
    15181 cggcgcgcac cgccagcttg cgttccaggc gatgaaattc cgccggattg atgtagcgca
    15241 tcgagaggtc ctcgagcagc gtgcgcatgc gcgtcatccc cagccggttg gccagcgggg
    15301 cgtagatgtc gagggtggac tgggcgacgg cccgctggcg cggcggcggc aggtgctcca
    15361 gcgtctgcat gttgtgcagg cggtcgcaca gcttgatcag caggacgcgc acgtcgcgcg
    15421 ccatcgccag gatcatcttg cgcaggttgc cgacctggtt ctcggtttcg gaggtgaaat
    15481 tgatggcgga gattttcgtc acgccctcga ccagctcggc cacttcctcg ccgaactgcg
    15541 ccttgatcgc cggattggtc aggaaacagt cttcgacgac atcgtggagc aggccggcgg
    15601 cgatcgtcgc cgggtcgttg atcatcccgg ccagatgcca ggcgacgcgg gcggggtgga
    15661 tgaaataggg ctcgccggac ttgcggcgct gatccttatg cgcctccttg gccgtctcat
    15721 aggccttgcg aatcagcagg acccccttgt cggacgggcg gggatccatc cgcgccagga
    15781 tttcctcgac gctcgggatg gtcaggctct ccggcagcag cagcttatcc acggagaggc
    15841 gcgacctccg ttcgccgcgc ggcgcggccc ccgggtggtg atggttgaca gccgacgatt
    15901 cccacggaaa accctcgcga ttgcacccga aggtccatcg ggctttgtcc cttcgtggtg
    15961 aagcgccggt tcatgagcga ctggcgagcg gttgttcttc tgggaaacgc catccgggag
    16021 atccgcgaca caagggacat ccagtcaccc cttcggcacg ccgccgcggg cggcgtaagg
    16081 gtttaaccta aaataacacg ttctggcagt cgccgaaaac aggtcaattc gggcgcccgc
    16141 gcgcggcgcc gcccgccggc cgcggccagt tcaggcttcg ctccgcttgc gccggatctg
    16201 ggccagcgcc ccggccagcc gcgcccgctc ttccggcgtc atccggtcga tgaacagcac
    16261 gccgttcagg tggtcgatct cgtgctggat gcagcgggcg cggaggccct cggcctcgtc
    16321 ctcgtgcgtc cggccctcca ggtcggtgta acggtaacgg atgcgcacgg gccgccagac
    16381 atcgccctcg acgcccggga tgctcaggca gccctcgctg cagacgtcgt cctcgaccga
    16441 atcgtcgagg atctcggggt tgatcagcgt cagcggattc ctgggcaggg cgcgccgccc
    16501 gttgcgcgag gcccagtcca cgtcgaccac gatgatgcgc tccagctcgc cgatctggtt
    16561 ggcggcgagg ccgatcccgc gggcttcgta cattgtctcg gccatgtcgc gcgccagcgc
    16621 gcgcaggcgg tcggtcacct gttcgatcgg gcgcgccgtt tcgcgcagga tcgggtcgtc
    16681 gtagatgcgg atcggcagca ggggcatcgc gtgtctccgg cgtgcatgca ctcggagact
    16741 actttagcgc cgccgcaatg agggcttcaa acagtttccg gtgcgccgga tcgtcggggg
    16801 cctgctcggg gtgccactgg acgccgatca ggaagccgtc gtgcggctcc agttccacgg
    16861 cctcgatcag gccgtcgggc gcccacgcca cggcgcgcag gccgggggcg aggtcgcgca
    16921 acgcctggtg gtgcgcgctg gggacttccc gttccccgtc gccccacagc cgcgcgagcc
    16981 gggagtcggc ggcggtccgg acggggtggc gggtgaagtg atacgcgccg ctgccgcggt
    17041 gcgcgatccg gccggggggg ccttcgagct ccagcgcctg ccacaaggtt ccgcccaggt
    17101 ggacgttgag ctcctggaag ccgagacaga tggcgagcgt gggggtggcg cggcgcagca
    17161 ggccctcgag cagtagaaag tccgaccggt cgcgcgcggg cgtcatcggc acgatcgacg
    17221 gggccggcgc cacgccgagc cgttccgggc gcacgtcgtc gccgccggtc aggacgaggc
    17281 cgtcgagccg gtccagcagg cggtcggtct cggcggggcc gctgagcgga gggatcagga
    17341 cgggcagccc gccggcggcg gcgaccgcct cgcagtagcc gtcgcgcaca ccggtgatgg
    17401 ggccgcgcgc atcgcggaaa ttgtcgcaat taatgccgat gagcggccgg gtcatgggag
    17461 gacgattccc ggttgggatt tgcggcgagg cgatgggcct gccgggggga tggcgaaggt
    17521 ccggggcccg cctcgtagcg gcccgatgat agcgtgattc gctggccgtg tctcgcggtg
    17581 aaatatcggg aaacctgggg ggcgttcgga ttggggaagg gacataaagg actgatggga
    17641 cataagggac agataaaacg tttcttccgg ggagaagcgg ggacggggga tgcggaggag
    17701 ttgaaatggt tgttcaggat ggctcgcaga cggctccttc gccgcgattg atcggtcgtg
    17761 caaaaagccg taaactgtgg gccgctgatc agatcgtcgg aatcggaaaa agttcgaggc
    17821 agcattcagc gccgggccac cgggcgtggg cggggacggg tatgttggca gcgtgtctgg
    17881 gggggtgcgg ggtattcgag agccgaggac gagggacgag agccgagagc cgaattttgg
    17941 agagggcgct tgggggtgat tgagttgctc atgaaccgga ggttcacaac gaggggacaa
    18001 gaatggggcg ggccgttggg ggcgaatacg aggacgagag acgaatttcg cgtgattgga
    18061 aaaccaacag aggagaagcc ccatggccag tgaaaatacg gtccggctgc accgcgtcct
    18121 gcgcgcggcg cccgagcgcg tctatcgtgc gttcgtcgaa cccgacgcgt tgatccggtg
    18181 gatgccgccg tacgggttca cggcgcagat cgagcagatg gatgtccgcg tcggcggcac
    18241 gttcaagatg tcgttcacca atttcggcac cggcaagcgc gaatcgttcg gcggccgcta
    18301 tctggagctc aagcccggcg aactgctccg ctacaccgat cagttcgaca atcccgacct
    18361 gccgggcgag atgcaggtga cgatcacgct gcgcccggtg gcctgcggca ccgatctggc
    18421 gatcgttcag gaggggattc ccccagtcat cccggttgaa ttctgctatc tcggctggca
    18481 ggagtcgctg ctccaactgg cgaacctggt ggaaccggag atccccgacg gtgagtgagc
    18541 cgcgggccgc gcgggccggt tgcggggttc cgctggaggg ggcatttcat cgccgtcgcg
    18601 gggcttgagg gctggcccgc gaaccttcaa aatgagtgga ggcgtgcgcc ggtcgcgccg
    18661 gcgccgtttg cagcggcggt tcaacctcca ccgcccggaa ggacccatga gttccgccac
    18721 gatccaacag gcccaggacg agatcatcga gcagttttcg cgcctcgacg ggtgggagga
    18781 gcgttatcgc aaactgatcg aactgggccg ccaactaccc gagatccccg aggagtacca
    18841 gatcgagcgg ttccgcgtca aggggtgcgc ctcgcaggtc ttccttcacc cggaatggcg
    18901 cgccgggacg gtgcacttcg atgccacgag cgatgcgatg atcgtgcgcg gcctgatcgc
    18961 gctgctgctg cgcgtctaca gcgaccgccg tcccgacgag atcctggcca cgccgcccga
    19021 ttttattcgc aagatcggcc tgagcgacca cctgtcgccg acacgggcca acggcctggc
    19081 ggccatggtg gaacagatca gactgtacgc gctcgcgttc aagcagctcg ccgagcgccg
    19141 ggcgggatag ggcccgaatc ggagatgaac ccaaggaaac gccgcgggcc ccggccgcga
    19201 gaatcgtgaa tcagaccccc aagccgttgc ccatgcgccc ctggttctgg tcgccgctgc
    19261 tgctgatcgg cgtcgccgca ctgatcatct ggcgctgggc gagcatggcc cagaccgaac
    19321 gcgagcaact gctggcgacc gtgcgcgagc accagcaggc atcccgggcg gaactggccg
    19381 aacgctgggg cgcgctgctg gactggacgc cgcccgccgc gggcgaaccg gcgcaaccgg
    19441 gggccgccgc ggcgatcccc gacgccgagc aggcgctggc cgtgacgccg cggcaccggt
    19501 tcagcgactt cgatgcgatc tacacggcct ttgtcggcga ccgcgccgtg gcggtgctgg
    19561 cgctgacgcc ggtggccgga tatttcaacg gcgagcggct ggatctggcg cgccattccg
    19621 acacgctgat cggccgcaag ctgaccgccg cggagcgcga gggcctgcgg gtgctgatcg
    19681 ggctgctgga acggcgactg gaccagtttg aggcgccgac cgaccgacat ggctggacgc
    19741 agaacgtgct gcgcacggcc cgccaactgg aaagcctcca ggagacgggc ggcggcgggg
    19801 cgacggacgg cgattagtaa gccggtcatc gcgacgcttc ccattcccga gttcgcctgg
    19861 atcggttctg ccgtccctgc gtgactcttc gttcatgctt cctgccgttg ccgccactga
    19921 agtagcgggc tgaagtctgc cgtccctgac gggactggcg gccctgcccc agaattcggc
    19981 tctcgtcgct tgcccacggc tcgcgaaaaa catgggcaac ttgcgactga aggtggcgcg
    20041 ggccgcgcac ggatcttgcg ttgcgggggc acggcgccat ccttcggccc catggagcgc
    20101 gtatttgaaa gaacttgcgg catcccgagg gtttcgccac gatggaaccg gagcctgacc
    20161 gaccgccgga tgcgccaggt cggccgaatc gcgtcacaaa ctttcgcggc gagagggcgg
    20221 tcctgttgaa gcggcccccc gcgccgccgg ccttgccctc accctcagtt tgatgacgaa
    20281 ggagaccatc gattcatgca cgccatgatg cagcggaggc cggtccgcct gttgccggct
    20341 cttcttttcg tgtttttgct cgccgcggtc gagacggttc gcgcccagga cccggtcgat
    20401 atcggcgtgg tcaccggcca ccgtcgcgac accttcccgc tgtatgtccc ggagtttcag
    20461 gtgcagccgg ggagcatgca gcagttgccc gagaacctgt tcgcgcaggt ggtgagcaac
    20521 gacctgcaac tgagcgggtt cttccggccg ccggacaacg tccagttcgc gcgcgagacg
    20581 caccagggcg acctgcagcg cggcgagatc cagttcgccg aatggtaccg catcggcgtg
    20641 ctctacctgg tgcggggcga gtatacgatc gtcgacggcg agctgcgcgt cgaggtgcgc
    20701 gtttatgaca ccgtggcggg cagctacatc ttcgggaatc tctatccaaa caaccggccg
    20761 gaacaggcgc gagcgctggc gcaccagatc agcaacgaca tcttcaagcg gatcacgggc
    20821 tacgacggcg tggccgatac gcagattctt tacgtgcagc aggccgattc gctggggcgg
    20881 accaagcaga tcggcatcat ggacgccgac ggctacggca tgcgcacatt gacgccgggg
    20941 ggcgagctta ccgccacgcc ggcctggggc gcccgcggca ccgagatcta ttacacgacg
    21001 taccgcgact tcaaccccga tctggagggg atgatcctgc gcacgcagca gcgctggtgg
    21061 gtctcgcgcc gcaacgggtt caacctgtcg ccggcgtgga acgaggcgct gcaactcatc
    21121 gccctgacgt tgaccaagga cggcaactcg gagatctaca cgatcgaccg cgaggggcgc
    21181 aacccgcgcc gcctgacgaa caacccggcg atcgattcct ccccctgctg gagccgggat
    21241 aattcgcagc ttgtcttcac cagcgaccgg accggcagcc cgcagatcta caagatgaac
    21301 gccgacggca gcagccagca gcgcctgacc taccaggggc gctacaacga cgcggccgtg
    21361 tggtcgccgg cgggccccga gcggatcgcc tacgcctcgc gcgtttcgga cggcacgttt
    21421 cagatcttca cctgcaatcc ggatgggtcc gatgtccggc aactgacgtc gggcggctcc
    21481 aataatgagg acccgacgtg gtcgcccaac ggcctgctgc tggcgttcac ctcggaccgc
    21541 ggcgggcgca agcagatcta tatcatgttt gccgccgatg ggagcaacgt ccatcggctg
    21601 accaacggca gtccatgcca ttcgccggca tggtcgccga tgaagcactg agggcgacaa
    21661 ggcggcggtc gatgcgattt caggggcgat cgcccggcag atccagcccg tcacgcttcc
    21721 cggcgacgtg ggtggcgggg ctggtcctga tctgcgcgct gggcggctgc acgccggcgc
    21781 gcagtcggct gggcgccatt catcagtatt cgacgctcga cggcacggcg gaggaagagg
    21841 caaccgagcc ggagcggttc gatgcggggc ccggcgccga cgcggcgacc tcgcccacgc
    21901 tcgaaccgct cggcgaggag atgcggccgg gcagccccga acactggggg cgcgaggcgc
    21961 gactggccgt cggccgcgag ttcgacccgc tctacttcga gccggggacg cttgaactga
    22021 cgccggcggc ggcgcgccag gtgatcgatt atggccagtg gctgacggcc aacccgcacg
    22081 tgtggatcac gctggcgggg cacgccggcc gccaggaaac ggtcgagttt gcgtataacc
    22141 tgggcatggc gcgggcgctg ctggtgcgcg agcggctgat cagcttgggc gtcgacgcgc
    22201 gtcggctgtt cgtcctgagc tacggctcgg agtggcccgc cgtggaggcg gagacgcgcg
    22261 aagccgagca gttgaacgcg cgggtggaag tgacgggttt cgtcgcgccg ctggcgcaac
    22321 cggcgccgcc ccccgaatcg atcgcaccgg gcgcggcccc cgccgtccaa gaggccccgc
    22381 cgccgttgag ggggcacgat attccgtagg ggcgtgttca agagcaaaac cccccatccg
    22441 ccctcggtct cctccccgcc cgccattgcc ttcaggcacg gccatcatta tcttggcttt
    22501 tgcgaagccg agtttggcca ccatccccag cgagagcccc gcacccatgg catccccgac
    22561 cgaagcgccc agcctgtggc ggcgaatccg actgaccctg ggcatgatcc gtttcagcca
    22621 ttcgattttc gccctgccgt tcgcgctgct gtcggcattt ctggcggcgg ggggctggcc
    22681 ggcgcgctgg acgctgggct ggatcgtcgt ggcgtgcgtc ttcgcgcgga gcgcggcgat
    22741 ggcgttcaat cgcctgcatg acgagcggtt cgaccggcaa aacccgcgca cgcgccattg
    22801 ggcactgccg gcgggcctgc tgtcgcggcg gtttgtctgg tgttttctgg tcttctgcgc
    22861 ggccggtttc atcttttccg cggcgatgct caacccgctg gcgttcattc tatcgccggc
    22921 ggcgctggcg attctgctcg gttactcgac cgccaagcgc tggaccgcgg gcagccattt
    22981 cattctgggg ctggcgctgg ggattgcgcc gatcggggcg tggatcggcg tgcgcggcga
    23041 gtggggctgg ccgccactgc tgctcgggct gggcgtcctg ttctgggtcg ccggcttcga
    23101 catgatctat tcctgccagg acgaagcgtt cgatcggcgc gtgggactgc ggtcgctgcc
    23161 ggcccgcgtg ggacgggcgc gggcgctggc cctctcggcg ctgtcgcatg cgctcgcgct
    23221 cgcgtgcttc ctcctcctga ccgggctgcc cggcctcggg tggggctacc tgctggcgct
    23281 gtgcgttgcg gcggcgctgc tcgtctacga gcaatcgatc gtttctcccg cggacctgtc
    23341 gcggctgaac atggcatttt tcacgctaaa cggatgggtc agcctgctcg tcttcctggg
    23401 cgggttctgc gacctcgtgc tgctcgggcc cggtccgatc ggatagttca ccatggcgcg
    23461 gcgcatccac atcggcactt cgggctggca ataccaacat tggaaagcgc gcttctatcc
    23521 cgccgacctg ccccagaaaa agtggcttga atactacgcg gcgcggatcg acacggtgga
    23581 aatcaactcg tcgttctacg cgccgccgag cgagacgacc gtccggaact ggcgcgcgcg
    23641 gacgccggcg gaattcgtgt tcacgtggaa ggccggccgc gtcctgacgc accgcaagaa
    23701 actgaaggac gccggcgaca tggtgccgga cgtgctcgcg cgggccgagg agctcggcga
    23761 caaactgggg ccggtgctga tccagttgcc gccgcgctgg aaggtcaatc ccgaccgcct
    23821 gcgcgagttt tttgcggcct ggcccggcgg gcggcgcgtc acgctcgaat ttcgcgatcc
    23881 atcctggttc accggcgaag tgctggaaat tctaacggaa aacagggccg cgctctgtct
    23941 gtacgaaatc ggcggccaga cggcgccgga ggaagtgacg gcggatttcg tctacgtgcg
    24001 gctgcacggc cccggccggg cgtatcaggg gaattactcg gacgcgcagt tgggcaagtg
    24061 ggccgatttc ttccggcggc agcgcgggcg caagcgcgag gtcttctgct attttgataa
    24121 tgacgacagc gcctatgcgg tccacaatgc tctgcgcctg cgcgagatgc tgcacctgag
    24181 ccattgagtg aggcccgcgg attggctgcg ccgcctgaac gaaatgcccg ctacacatcc
    24241 tgtcggctcg cgaatacggt tgagccgcac ccgcggcgtg catggggact gcggcgttgg
    24301 ctcgccgtcg cttgccttgt tccgttgatc ggcgcggaaa ttcgggccgc gccacgggtc
    24361 tacgtgatgg acgacatggc gaaggcgacg ggggatgagg cgctggcgca ctccgcttcg
    24421 gtttattcca atggaatgat tgagctgcat gccgccggga acgaagtggt ggcgtttcaa
    24481 ctggtgtttc aggcggatcg ggtggaacgc ggcctcgatc tgcgcgcgag cgatttgcgc
    24541 ggaccggcga cactggcggc cgacgacgta ttcgaattct tcctcgcgca ctatctgtcg
    24601 acaaccgacg ggagctacgg ctggtttccg ccgggcacgg ccggcgtctt gccgtgggag
    24661 gggaagctct ggcccgatcc gctgattccg ttcaaggatc cctggacgac gcccgccgcg
    24721 gcggtcgcgg cgccgtttgc gatcgatccg gcgcggcagc ggaaccaggc ggtctgggtc
    24781 gatgtattca tcccgaaggg aacgccggcg ggatggtatg aggggcagat tgactgcctg
    24841 cgcgcggggc ggccattccg ggaaattcca ataagaatcg aggtgtttcc attcgatttg
    24901 ccggacgagt cgcacgccga cgcgtggggt gaactgtacc gggaaacggg cgtcatgttc
    24961 gattccggcg tcaaattcaa ggagcacccc gaggccgact ggcccatcta ccggcgctac
    25021 cagcaactgg cgcacgcgca ccggctgctc gccacgcagc gccaggggat gggccccgtt
    25081 ccgcgcacgc ccgcggggcg gccggcggac cggccagacg accagtgggg cgaggactgg
    25141 tcgctgttca cgccctatgt ggcgccggtt ctggatggga cgctgttcac ggagtcggcg
    25201 ggctatcgcg gtccgcgcgc gggcgccggc ccgactttct ttccggcgcc gttcgtggag
    25261 accttttacg gcgcggattc gctgcgcgcg catctggaaa aacacaaggg ccggatcgat
    25321 ccggagctat tggaaacatg gacccggaat gcggcggcgt tctggcgcga agtgcagcgc
    25381 cagggctggg aggaccggcg cttcttcgcc tacctgcttg atgaggtcga ttcggcgcgc
    25441 gacggcgagg gcggggccaa aataccgatg gacgagatca cgcgctttca cgcggcgatg
    25501 cggtcgatcc aggcggccct ggacgcgggg accgacggac ggcggcggat tcatctgctc
    25561 tggacctcgc acgccgacgc gacgcgctgg ctcggcacgc cggccgacct gacgccgttc
    25621 atttcgtggt gggcgcccaa cgggcacgcc ctgcagaccg attttttccc gcgcgccgcc
    25681 gccacgccgg gccacaccgt ctggttctat cattccggtc acccggccgt tggcaaccac
    25741 ggcatcaatc agttggggat cgacctgcgg ctgtggggcc tgctctgcgc gcgctacgcg
    25801 atcgacggct tcttcgtctg gtcgttgatg tcgttccccg agcgctggga cgccgagggc
    25861 ttcatcccct acaaccatcc cggctatcgc gaaggggaaa atcgctgggg caacgggacg
    25921 ctgctctatc cgggatcgcg gctgacggcg gtcggcgccg cacgcgcgat tgaggggccg
    25981 atccccagct accggttgaa ggcgctgcgg cgcggattgc aggatgcgga gtatgcgtgg
    26041 ctggcgcgcc gggccggccg cggggaagcg accgacgggc tgctgcgcgg gctgattccg
    26101 gcggccttca gcgaagcgcc gcgcccccgc tggtggcggc cccggccgca cgggctgtgg
    26161 tcgaggcgcc cggaggacta cgcggcggcg cggctccaac tggccagatt gattcaggaa
    26221 ggcgccgatc cggctgattg accgggcggg cgccgatggc agctccgcgt cgctcagcgg
    26281 cgcgcgagct gttgggcccg ttcgatcata tgctcggcca cccgccggac ctccacccgc
    26341 tcgcgttcct cggcggtctc cagcaccgcc gccaccagcc gctcgcactc ggtcttgatg
    26401 gcggtgtagt cctccggatt ttcgccgcgc agcttgagca gggcggagag ccaggtcttg
    26461 ccgtagctgg tgtggatcga ctcgtcggcc cagtcaaaat ccatgtcgtg ctggctcatg
    26521 cggtcttcca gcagctcgaa gctcttcttg cgctccatct tcttgccgat gttcttcgac
    26581 tcgaagtaga agagcatgcc gatgcgatag agcggatcct gaccgcggca actgtcgtag
    26641 atgtaagtgc cgagcggcag ctcgcggtct tcaaaacccc acgtcttcag ccgttcgtac
    26701 cccatgcgga catgccggct ttcgtcgtag acccagcgcg cggcatcgcg gatgaactcc
    26761 cagccgagat cgtcggcgta ggtgtggagg atgaagccgg cgttctccac cgcccagacc
    26821 tcgttgaagt ggctgacggc gctcctgagc tgaagccgca atccctcgcc ataggggaac
    26881 gtgggatcga tgatgtcggg ccagtagaag cgcaggttgt ggaactgggc atcgcgcgcg
    26941 ggggtttcgg ccggagtgaa cggccgttcg agcgcgggca ggtccgaccc ggcgcgcggg
    27001 ggatccaggc cgatgccgcc gatctcggcc agcagttgct ggagccggcg gacccagtcg
    27061 gcgctgcgcc gcccttcctc cggatcggtt tcgaagacgc gggccaggct cgcctcgatc
    27121 tgctcgatat ggccgcgcaa ttcctcggtc gccagccgca tgaagcggta ggtcggcccg
    27181 tcgccaagat cgtcggccag ctccagatag gaattgaaag ccttccgcag cgccggcttg
    27241 aacaccttgg ccaacgccat caggaacgcc ggagcggagg gcgccgcctg cgcgaaccgg
    27301 agcagattga ccagcggcgc gtcgtcgcca atctccagca tgcggttcgg gaagcgcagc
    27361 tcgaagacgc gctcgcgcag cgcgtcggcc gtcagcgcgt cctcccagag catcttgggc
    27421 agggcggttt tcatgttgaa gtcggcgatg ccgggcagcc agccggcctg gctcaccacc
    27481 agcatccgct cgaccagaaa cagccgcttc aggatcacgg cggtatcgta gcgcatatag
    27541 gttccccgga cattgccggc caacgcttcg acgaccatgg gtggatcaac ctttctggct
    27601 caagaatgca gaacgcatga agggaacgaa tgaggccaaa ggacacaacg gagttgaggg
    27661 acggagggaa cgacccattg tcccttccgt cgcctcagtc cttcatcttg ccggccggct
    27721 ttactcgccg accagttgtt cgatctcctc gcgcgagagg atgccatcct gcatcaggct
    27781 gagcgtttcg gccgtaaccg cgccgaggct aacgaggcac tcggtgaaca gctcgcgggt
    27841 ccggcgttcc aggtcgcgcg aacccagatc ggtcatgacg ctcaggatgt ggcggccctt
    27901 gcgaacgtgg gtgcgctcgt cggcgcggat gtagtcgctg tcgtgcgcca gcaggatgtc
    27961 gttggccagc ttcgccgagt cgtgcaggcc gttgatcgtc ttaaggatcg agacttcgcc
    28021 gaacaggttg atctcggcca ttgcgaaggc ggcgttatcg ccggtcatct cgccgcgaca
    28081 ggtcgagctg gtcagccggt tgggcagttc aaaggggttg tagcccatcg cctgcatggt
    28141 ccggtggccg atctcggtgt ggcgcgcctc gtcccaggtg atccgcgcca gggcgtattc
    28201 ggcgtcgaaa tccttgaagc ggatgtccca gaggaacgtg ccgaaggctt cgatcgcgtc
    28261 gacctcgtcg cgctgggtgc ggatgaagtg cagccggaac gattcatagc tgccgcgctc
    28321 gaaccgcggc tcgccatcgg ccgcgtcgta atcgccggtg tggcggaagg tcgtgaaccg
    28381 gctgtcgcgc agcggaacgg tcccgcgttc gtagggacgg gcggcggacc gcagcggcga
    28441 gggttgctcg gcgcgctccg cggtgccggt cacgccgccg atgctggcga gcagacgctg
    28501 caggtgccag cgccagacgc tcagccgcga ttcgtcgacg ccgccctcga tataggcttc
    28561 gatcgcctgc tcggcccatt cgagcatcgg ttcgtaatcc atccggatcc ggcgcaggat
    28621 gcggatcgtg ggggcgtcgg tgatcggatc ggtcaggtcg caatgatggg cgtaggcgag
    28681 atcgagggcg cgcccggcca cctgatgcac gccaacgagc aactcggggg cgtcggcggc
    28741 gctgagcatc tcctcgatga agcggtcgat ctcctcatta cgataggcgt cgaccttggg
    28801 cagcgtcatc tcctgctcgg tcaaacgctc gcggagctga tgcgcggcgt cggcgtgata
    28861 gaagatatgc cgtccggtct cgagcttgac ctcgaaatcg gggatcgaca gcgtccacga
    28921 accgagggcc tgggccaggc gccgttcaaa atagaaaaag cgcgacagga tttcggcgtt
    28981 ctgctggacg ctgaacttgc cgccgagctg acggcggtcg cgggggaaac gatagggata
    29041 ggctttctgt cgcgcctcgg ccgcctggcg ttcctcagga cgcgactgaa ctggaaattc
    29101 accggatcga taggcgctct caaggagccc ctgcttcgtc gaaactgcca tgaccacacc
    29161 tcccgttatt gcggtagcac aagacgctgg ctgcgcgatg gctaagactc taaaataaag
    29221 ctgatttcgc tgtcaagtta tagttaacag gtgataattc cgggccgggg ggggcggccg
    29281 caggcggacg caggggggct cccaccgcga tgagagcacc caagttgatc cacggaaatc
    29341 tggggaggaa agggggcgaa atccgtcgga ggggccggct ccggccgctc cgacggattt
    29401 cggaggtcag gatggatggt ttacggatca gccggccgtc aggcgccgcg ccgaatccac
    29461 atctcgccgg ggccgcggaa ctgcacgcgc ggctgccatt tgaccgtctc cggctccagc
    29521 tcgacatcgg ggcagcgcgc cagcatgatc tccaggacct tgcgcgcctc gagccgcgcc
    29581 agcggcgcgc ccaggcagaa atggattccg gccccgaagg cgagatgctc aatcttgccc
    29641 cgctggatat tgaacacgtc gggctggtca tagtgccgcg gatcgcgttg cgccgagccg
    29701 atcaactgga acaccatttc gtcctcggcg aagggcacgc cgaggaactc gtgagcgcgc
    29761 ttgacgcggc ggaagttgcg ctgcttgggg gcgtcgtagc gcagggcttc ctcgatcgcg
    29821 ggggtgatca gattcgggtc gcggacgagc gccgtccact gatcgcggtg gcgcagcagc
    29881 agatggagca gattgcccag cagattggtg gtcgtttcgt ggcccgccgt gaggatcgtg
    29941 ttgcaggtgg ccagcagttc gtcgcgcgag aagcgctggc cttcctcctc ggcattggcc
    30001 agcgccgtga tcaggtcgtc gcgcggctcc acccggcggc tgtcgatcag ttcgttcatg
    30061 tagtcgaaca tttcgagcag gctggcctgc gagatgcgcg cggcgtccgc cgtcgtgacg
    30121 ccggtcccct gaaaggcgag gatatcggcc gaccagcgct tgaacaggtg gcggtccttg
    30181 accggcgcgc ccatcatttc cgcgatcaga atgaccggca gcgggtgggc gacctccgcg
    30241 atgaaatcga agcgccgcga ttgcagcgcg tggtcgagca gctcatgcgc cagggcctcg
    30301 atgcgcggcc ccagccccgc gacggtcttg ggggtaaacg ccttctgcac cagcgcgcgc
    30361 atccgcgagt gatcgggcgg gtcggacccg atgacgtcct tctgcgcgta ataatgacgc
    30421 agcggcgcca cctcggcgcg ttcggcctcg gtcagcgcgc tgaacagcag cgcctggcgg
    30481 ttctcgttgg acaggttgtc cttgtccttg agcgacgctt ccacgtcgtc gtagcggctg
    30541 atgacccagg cctgccaggc gtcgctccag aagacgggct gctcctcgcg caggcgttca
    30601 taaatcggat agggatcctc catgatctcg tcggagacga gcagcgcatc gagctgggga
    30661 tcgggacagg catgcggact attcatcgat cggcctttca agagtcgctg cggcccgcgc
    30721 cggcggcgcg ggtcattcct tgtcggcgag gcggatggag acaaacgtca ggggcttgcg
    30781 gcccggtcgg acgccggtca gcgccagcga ttcaaagggc cagcggccgc ctccctcgcg
    30841 gacgcattcg ggcgcctcga aatccaccca cgggatcacc agcgtcgttt cgccctcgcc
    30901 atagggcacc cgcgcttcgt agatgttggg aatgttgcgc gtcttgtcgt cgtcgagcag
    30961 ttgaacgcgg agttcgcccg cctggccctc gccgttgacg acgatctcca agccggcgaa
    31021 ctggccggcg cgcgggccca acagtaccgt gctgtcggtg cggaccagga acaggtcgtt
    31081 cgaaccgggc gtcacgcgca agccttcggg gccggccggt tcgatcacgg tttccttctt
    31141 gtagatcgac tggcgccagg cgcgaatccc cgccgacacg tcccacagcg gattgatcgg
    31201 ccacaggttc acccagtcgc gctgggggtt cttcccctcg cgccacatca cggggctgga
    31261 gacgcagcgg ttatcgctgg cgcagaccgt gacgaagtag ttcccggcca gcccgcgcag
    31321 gtgctcgggc aactcggcgc gccacgcctg gccctcgcgc cgggcgggga gttccaccca
    31381 gtaggtcgac cgccagttca catcgccggg gctgaaatac agatgggcgc cctcgacctg
    31441 cggcccggcc gtttcccact cgaacacgtc gggggcaacc tcgcggaccg actcgcgggt
    31501 gacctccggg aacgggaccc cctcgatgtg gtgctgcaga atcgaatagg tgaacggcca
    31561 ggtggcgatc ggctcaggga acgtgccgat gccgtgatcg gagttgggcg taatgatcac
    31621 gcgcttggga ccgggaaacc ggtcgaagtg gcgcatgacg ccgccaaacc agaagaacga
    31681 gtcgttggcg tgggccacca gcgacacatc ggcgcggacg ttgtcggcgt aggaaatcgg
    31741 atcgaactgc cgcaaccact cgtcgcgcag cgccgggggc agcaggttga tcccgtcggc
    31801 gatgatcgag cggctgtcgt cccagccccc cgcgccggtc agcgccaccg cgtcggtgat
    31861 ccgctcatcg atgccggcca gcagcgtcgt gtaatagctg ccccacgacc cgccgtagac
    31921 gacgaccttg ctcatatcaa ccatcggctg ggtggcggcg aagtccagcg cgcggcgcat
    31981 ccccatcgcc gtgcggtagg cgctgctgcg ggtccccgtc gtcgtttcgg tgccgggctt
    32041 gggatagcgc cagacggtgc gcaggggatt gggatcgctc tcggatttgc caccccagtc
    32101 catcgccatc gcgaagacat cgaattcctt ggcgaacgac agcgcgcggg ccgacgtgcc
    32161 gacgccgccc cccccatgca gatccaacag caccgggaag ggaccctccc ggtcagccgg
    32221 gaaggccacg cccaggtaga cccggttggg gccatccggg ccggactggc tggtgaagaa
    32281 gacatcctgc acctggcagc cgtcgacgac cttggaggag tagatctcca cgtcgagggg
    32341 cgtctcgcgc aagggcgaga tatcccacat cgcggcctgt tccggcgtca gtttgctcat
    32401 cggagcgttt tcctccgccg cgcggcaggg catcgcaacc gccagcaaca gcgcgccggc
    32461 gcacagcagc cgcaatccat ggcgcatggc atcgatttgg ttctttcgtc cggtcatcat
    32521 gacgcgtcct ccgcctcccc actggctcgg aattcgggct gatacattcg ccgcaattcg
    32581 ggcgtcacgt gattgagttg cgcggccgcg atgttgcggt cgcggaaggc ctcgtgatcc
    32641 agcgccgaga gatagcgctc ggaccgcgtc ggcgcgccgt cggggaattc caccgtgtcc
    32701 gacagcagat gcatggccag ccacgcatcg aattcctcct cgcccatcgc accggcgaac
    32761 tcgtccgaat ccaggatgcg gcacagggcc tcaaaccgag tcagccggcc ttcccgcagc
    32821 gacgttacaa ggtcctccgt caaagcgccg ctccccgtca tgccgcatga agcgaagatg
    32881 ctttcgatga attcctcatc cgtgacggtg aagcggtact ccagcgaatc gaggatgcgc
    32941 cgcagaatgt cgtgcggcgc gatccggtcg taatccagcg ccagccagct ggcttcctgg
    33001 tccgtcggat tgcgttcgag ccagacccgc gtcgcattgc gcaggaagtg catcttggcc
    33061 acgtagtccg ccagaacgta gtagtcctcc gtcttttcgc cctccagact gaagagcgcc
    33121 gagttgtcgt tctccagttg ggtgtaggtc gaccagaaca ggttgatcgg gctgccccag
    33181 cttgccaccg ccttcagccc caggcggttg cggtggggac gctgcggcac cggcggcagc
    33241 gggagcccgt attcgccgat gaacacc
//
LOCUS       DAOPCO010000012        32830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1496_length_32830_cov_5.202075, whole genome shotgun sequence.
ACCESSION   DAOPCO010000012 DAOPCO010000000
VERSION     DAOPCO010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 32830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32830
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_32830_cov_5.202075"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..670)
                     /locus_tag="PLS90_01950"
     CDS             complement(113..670)
                     /locus_tag="PLS90_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01196.1"
                     /translation="MKTALESYVVDNNEYPRALPIYSFLYLYTYCNFLRGYDRTNMRD
                     IGAWELTTPIAYMTDLPLDTFTEHNRYGGGNLNRLGFAYVNFKSMAKNPFKGSFAYPD
                     LPEAPALRLDWDAGAKRQTNYLLICAGPDYESNFPWIVNNGQWTMLQAMTEEPRPSGI
                     AEVARLITYDPTNGTVSTGDIYVAQ"
     gene            complement(667..846)
                     /locus_tag="PLS90_01955"
     CDS             complement(667..846)
                     /locus_tag="PLS90_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01197.1"
                     /translation="METRIGLLQRARRDRLAFTLIELLIVVAIIAILAAIAVPNFLEA
                     QTRSKVARAQADLRW"
     gene            complement(940..1734)
                     /locus_tag="PLS90_01960"
     CDS             complement(940..1734)
                     /locus_tag="PLS90_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01198.1"
                     /translation="MRQFTLGALMLLALLPAARAGTIVWDFDGTETSTEGLPAGAIDG
                     DLLVTEGWLPSPQQALIGIPATDFEVEGGLLTQRFSTLTPGQLPTSNPSHIHSTGIKV
                     GDLRGALVTSGVTPTVLNLANVETEYQYLVISGRFGWASAGGEVGIIIYPVTEEAENW
                     FTISLRITTVQTNITSRQVFDLTQSPRTSTYAGTLRGMLMNLYGMWNFVSGEQVCDPT
                     FMRDKWINEFNPTDTVVELDWIALTSDPDFRLGETVNDASRWNIFE"
     gene            complement(1825..2598)
                     /locus_tag="PLS90_01965"
     CDS             complement(1825..2598)
                     /locus_tag="PLS90_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01199.1"
                     /translation="MMRMVSCLGIMILLALSAAVGQAGTIVWDFDGATAGLEAGAIDG
                     DTHTPEGFRPNPTQFNVGLSASNFNVENGRLIQTYAELPTGEVPTTDASYIHCTGVIV
                     GDLRGVYPGATTPTLLNLTNIETDYPYLMLGGIHEWAPAGGDVGMILFPVTTEAAAWF
                     TNSLRLSNVVTNTYGRQVFDLTTSIRTGSYSGTLQGIMFDFYGMWTAAVDPTFMKAKW
                     ESEFNPATTRVELDWVALTDDPDFRASYVPVELSRFTLE"
     gene            3069..3860
                     /locus_tag="PLS90_01970"
     CDS             3069..3860
                     /locus_tag="PLS90_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPK01200.1"
                     /translation="MNSYQTVEKTFRILELLATNAPLGVTEISIELGIEKSSVSRLLK
                     TLFGLGYVVQSGHRGRYQLGPRLLYLGDCYLRENRLLKEAEPILQELAFATRSSAHLA
                     VRVGEQLMVLAKEPSPERIQVASSIGSPVSPHASALGKVLLAGMDEKERQRFLPEELI
                     PFTEHTIVDRRRLLEALDEVRRNGYALETSEEVMGVGCIAAPVRDSADRWIAALSVSG
                     PLHGTAFRQDARSIELVVQKALALSALMGYQPDPQTRARMELLNR"
     gene            3881..5245
                     /locus_tag="PLS90_01975"
     CDS             3881..5245
                     /locus_tag="PLS90_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPK01201.1"
                     /translation="MQTSTGLRPPRNDKPSAAGEPTAAEIARRAAQCIAGGAVSLNRK
                     VEPELLFVRAAGSRLFDADGRQYIDYHAAFAPHLLGHNHPVINGAVREAMERQWSLMG
                     SGTTPWEVRLAELLCAAVPSLETVQIANTGSEATAHAVRLSRAWTGRNHIILTLGGYN
                     GWHNEVARAVSPSLEQVGPRVSPGEYRFIPLSAGIPESTRSLVHLVNFNDLASVEYVM
                     RRYPVACVMTEPVLQNIGVVPPREGYLQGLRDLCDEHGALLVFDEVKTGFRSALGGFQ
                     SICGVLPDLSLFGKAVANGFPLGVIGGRRDVMSLFGDPDPARRVLIAGTYNAHPFTSA
                     AAIATIEYLRDQPVYAQLEALGARMQAGLERIFAEHGITATVSRIGSAFCVYFCDHVP
                     VDYHDLLLHHDFTFDMRYRRELIDRGIYHFPIACKQSSISAAHTIEDIDQTLEITRDV
                     LREI"
     gene            5433..6611
                     /gene="dgoD"
                     /locus_tag="PLS90_01980"
     CDS             5433..6611
                     /gene="dgoD"
                     /locus_tag="PLS90_01980"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HPK01202.1"
                     /translation="MKITAVTTFVCHARMRNWVFVKVQTDQPGLHGWGEATLEWHTRE
                     VVGAVEDLAELLIGQDPTRIEHLWQTMYRQHFWHGHGIVRSTAISGVDIALWDILGKL
                     HGTPCHRLWGGAVRDYVRTYCHLGGGRMEDFYETAPSDAGRFADLARQAVEEGFTAFK
                     AMAVPETMPIEGRRAVRYAEACVGAMRAAVGEQIDIMIDCHARPSPRMGHLFARALEP
                     FGLYFLEEPCWPEHLKDLAEIQRAVATPVATGERLTGLHAFRDLLELRACSIVQPDIT
                     HCGGLTVARKIMALAEAHQLAAAPHNPQGPVSTAASLELGFASPPYVICETVHADVPW
                     RHDVVTEAHTIEREGRIVRPHQRPGLGIEINEREVAKHPFQPEVLLRTFHADGSVGDW
                     "
     gene            6611..7408
                     /locus_tag="PLS90_01985"
     CDS             6611..7408
                     /locus_tag="PLS90_01985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK01203.1"
                     /translation="MANLAGSTAIISGGLGEIAQAIALAMARAGANVALGDIRAPQDA
                     EPILAQLRASGVRARYDHVDIAEPAAVERWVRDAETDLGVPDLVISNAATCTAASVRS
                     ITPEAWNRELDINLNGALYLSQYAARRLVTANRPGRIVFISSWAAEKAHPDIPAYCVS
                     KAALRMLCKCLALNLAADGILVNELALGFVNAGLSGRAFRLYPEVQARSTAWVPIQHL
                     IEPEEVAAEVLHLCDPAVHHMTGSVVLMDGGLSMTNISHASRGYGKD"
     gene            7415..8848
                     /locus_tag="PLS90_01990"
     CDS             7415..8848
                     /locus_tag="PLS90_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline ceramidase"
                     /protein_id="HPK01204.1"
                     /translation="MAQQPPAVHHAGFHGRIGVARREITPPVGIYARCWAAARHDVAE
                     GIHRPLTLTALALATAEDPTPHVLITLDACSWGAPEAEWGVRGEVIRRLGVDPARVIV
                     ALAHTHSGGPAFLSPSMDGFPGGEHAETFYHGIVQAAGEAAVAARDASIPATLEWEYG
                     RCALAANRDLPLPAGYVCGYNPGIAADDTLLVGRVTDAEGRILATLVNYACHPTTLAW
                     DNRLISPDFPGALRETVEGATAGVPCLYLQGNMGDLAPREQYQGDPALADRHGRRLGY
                     AVLSVLEGMLPAASRLEYQGAQRSGTALGVWAAVPHVHPGDLASLTFDLDLPLKDDFP
                     RDEELERLQATTNDRVEAEKLRRKRTVRRWIGNGASARLSGWVWRLGDALLVGLPTEP
                     YSQLQVELRRRFPCHPVCALSLANGSIGYQPTAAMYGTDVYQVWQTPLARGNLELTLE
                     ECSRRFQELLEGEPARAETARGGSAGF"
     gene            9051..9893
                     /locus_tag="PLS90_01995"
     CDS             9051..9893
                     /locus_tag="PLS90_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK01205.1"
                     /translation="MTAPITPAQIRQFHDDGYLMLPGLFSQEEMDLLLSAARNDRALE
                     ENAVGLTDGTGRVSKLSLWYIPRDDIYGLFSRSARIVDTAEQLLGDTVYHYHSKMMLK
                     EPRTGGAWVWHQDYGYWYEAGYLYPDMLSCLIAIDRASRENGCLQVLKGSHKIGRIAH
                     KLVGDQTGADLERVEQARKVCELVYCEMEPGTALFFHSNLLHCSAANTSDRSRWSLIS
                     CYTAAHNLPYKQVDHPGYEPLVKVPDSAILEAGGRGLSERTEFLSRQKLEGYNRSRAA
                     QANN"
     gene            9922..12105
                     /locus_tag="PLS90_02000"
     CDS             9922..12105
                     /locus_tag="PLS90_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPK01206.1"
                     /translation="MQLQLLDIIIFAGYFIGIIVFAIVVAARSRSRDAASYFLASNQL
                     PWYAVGASFIAANISTEHFIGMVGWSFLYGMSIANWCWLNAVTFSALIWIFLPFYMRG
                     AIATMPEFLERRFNRFCRYAYALLTVIGLVIALLGGVFFAGAKAITVFFPEVSTTAAI
                     IILAIAAGTYTIYGGLLSAVWADLLQYILLMTGGLVAAIYGLYYAGGLDELMQHLPEK
                     FIILYPSTHEMIPWTGLLMGIPSVGLWYSCANQFMVQRCLGARSEWDARMGVVMAGFS
                     QAILPLLIVIPGIAAFYLFHDQLSDGDQSWPFMVRQFLPAGLVGLVLAGLTSAVMSTL
                     SAITNSSATIFTLDLYKNIVRPHADDREIHRVGRISGAAAMFIGVIVAFVMARAEGAT
                     VFGLIQTVFYYLAPPIAAVFLVGIIWWRATPAAATAALTLGFAVYLPLVVFVIFPRIP
                     LLAPYDNFMHHTFGVFLLSVLTIIIVSLFTKPKPREALKGVIWTRSALAVPEGERKRH
                     TGIRSLGLWWTIMVVLTIGLYAYTYSVGSNSEALEAERLAYTTSSGTAPRVQRKTELK
                     NFNLWTGDGQVLFEPQRDGERLTFTLPITEPGRYQLDALVTKGPAYGQYDIEVQGQPA
                     EIQYNITERSGAGHYSVRHLAASTFDARHPAAAAPSGGSASQIESIAGEHVVQRISLG
                     SFAFDNPDIATVSFVARHVEPGHALIGVDLIALTRTGDLPDPTKE"
     gene            12110..13162
                     /locus_tag="PLS90_02005"
     CDS             12110..13162
                     /locus_tag="PLS90_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPK01207.1"
                     /translation="MQGQYFSRVFARTPTRLWINNPTGDDLTRALNVGCIAGTTNPSY
                     CAKLIDQEPEFILPIIDEEVQRHEDDSVAADRVVQAATRRFMDAFRPIHGASGGTLGF
                     VTIQDDPARDHSAERIVEAALVHQRVGPNYMAKVPVTEAGMEAMDELISRRIPVCATE
                     CFGLDQAITMCERYERAVARGGHRPPFFITHITGIFDDELKDFAARHNVQIKPEILNQ
                     AGCLVGRKQYHVLRERGFPAVMLGGGARGLHHFTAFVGGQMHITLNWSTIEELLDADE
                     PGDCRIDDLPADDVVAELCDKLPDFRRAWHEGSLRPEEFESFAPLQRFRNNFLAGYGR
                     LREEVARRRAAAMARP"
     gene            13159..13689
                     /locus_tag="PLS90_02010"
     CDS             13159..13689
                     /locus_tag="PLS90_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK01208.1"
                     /translation="MTRTLPTLWDQIGAHWPANSHDRAWPYGELAEAAHAFLAGHGVN
                     PVGLSHYGIVVADRNAALARLNRLAQEPWRPTTAAWVEAFNVHVMRGDLQGVELEFIE
                     PDGPSFFADFLAGQGERLQHISFRVDGIHEAVARLRDAAVPLVNDQVREGSHGWIAFL
                     RPDPFAPLCLELCQPK"
     gene            13703..14953
                     /locus_tag="PLS90_02015"
     CDS             13703..14953
                     /locus_tag="PLS90_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01209.1"
                     /translation="MNDSPSSAPRSRRQFLGDAAACAFLSGALLPAAGALLAAPAARA
                     QAASSQSDPANKIRIGIVGGGFGASFHWHLDPGCIVHAVSDLRPDRREILKQTYQCDL
                     AYDSLEELILDPRIDAVAIFTGAPDHARHTIAAMEQGKHVICAVPACLTLEEAEALLD
                     ARRRTGRRYMMAETSYYNPHGIAMRELYRADAFGDIFFSSVEYYHPMSDEERRQHWFY
                     DNQRTWRHGFAPMLYPTHATSYLVATTGERLTEVSCRGFLAPAIEGYGVGQNAYDNPF
                     NGQVALFTTDRGHVCRCNVIWTGSIPTAERAELLGTKLSYFMPIDALDQPARELAAGG
                     ARGVALPDYSQRLPEPMRVSSGHANSHPFITHEFIAALLEDREPEIDLHRSLAMTVPG
                     IVANASSRRNGERMTIPDYGGGSV"
     gene            15066..17165
                     /locus_tag="PLS90_02020"
     CDS             15066..17165
                     /locus_tag="PLS90_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HPK01210.1"
                     /translation="MNRNGKLRIGADVGGTFTDIVLMDGDGAVWTRKVPSTPPNYEQG
                     VLDGIAALLKTVPNAAGERVGEVAHGTTVATNAILERRGARTALITTEGFRDVFEIRR
                     LRVPQLFNLFFEKPPTLIERFLRFEVAERVAATGEILKPLDEDSLGLLVDQLRRHQVA
                     SVAVCLLHSYAFPDHELRVGDYLRRHLPGVDVSLSVHVLRERKEYERTATTAVNAYVL
                     PVMRGYLSALKDGLERQRIRATLQIMQSSGGLTPDEDAARRPVYILESGPAAGVLAAR
                     FIARHLGLPRVITFDMGGTTAKASMIEHGGISYSPEYEVGSSLSCSSRLVGGAGELIR
                     VPTIDIAEVGAGGGSIAYLDRAGGLHVGPRSAGALPGPVCYRRGGTAPTLTDANAVLG
                     YIRPGRLAEGDVDVDVEAARRAIHDQIARPLGLDLFEAAEGIHRIANAVTMRALREVS
                     TQRGRDPRQYVLIAFGGSGPIQAAGLARELQVLKVIIPPLPGLFSAAGLLFSDVEHHD
                     IRSCLLSGDSLRADAIEPIRREMQRAMRERFQREGYAPDRVVLSTSADVRYKGQFSEI
                     RVPLDDAEITAQTLEHLRTAFEAEHHQLYGHSAGAESPIEVVSVRLIGSVPAETGRTA
                     LRPAPRAGDSPASRPVWFGARWGAVETPVVDRAAIGEHACGPLLIDEYDSTIVVPPDM
                     TVTLDDQMDIIMEACRG"
     gene            17158..18903
                     /locus_tag="PLS90_02025"
     CDS             17158..18903
                     /locus_tag="PLS90_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HPK01211.1"
                     /translation="MGELDPITREIIKNALATAGDEMAITLYRTAYSTIVRDCLDYST
                     QLTDADGNMIAQGVTIPLHIGSAPFAMKSLFEKFGDDIEPGDLFILNDPFDGGMHTPD
                     IFIVRPIFWENERVAFAIATAHHLDVGGRVAGSSACDNTEIFQEGLRIPWLKLYRRGQ
                     PDASLFALLRANVRVPEMTLGDLQAQIAACMTGERHIHEIIGRYGLATFRECTRWLID
                     YTERILRAEIATWPDGQYSFTDYLDSDGVGGPPVRIQVTLTVKGDELTADFTGSSPQV
                     RGAINSTLSYTASVTAACVRSLLGEELPNTAGMFRPLRIIAPEGTVVNVVMPGASSMR
                     GVTGFRTVDTVFGALAQILPGRVMAAGEGGNTLVIFGGQRENRAPYVYYELLTGDWGG
                     RPDRDGCDALCNPVNVASNIPIEEAECAYPIRIEHYGLARDSGGAGRFRGGLAVQRQW
                     RLLSGEASLTIRSDRRDHPPYGLHGGLPGQPSINILYRDGEQTILPTFSSGSIRAGEQ
                     LYHRMPGGGGWGDPLDRLPEAVAHDVLNDKVSREAARVLYGVAVDEQCRVDEHETTRL
                     REALRQQRTGAAATT"
     gene            18981..20291
                     /locus_tag="PLS90_02030"
     CDS             18981..20291
                     /locus_tag="PLS90_02030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK01212.1"
                     /translation="MSKYDVIIIGAGIMGAATAYELAKKGYKVVVIEKNAQAGTGSTA
                     GSCAIVRAHYSTYQGVAMAYEGFFYWLNWEDYLGVKDPEGMARYMNCGSILLKTKGHN
                     YRKVLEHFRAAGVKHEEWTLEQLRENMPLYNHKAWWPPSRPEDTPDYWDREPTDTIDG
                     AVFTPESGYVDRPDLATRNMIHAAKAHGAELMTGRRVVQIHREGNRVQGVSLDNAERI
                     DAPIVVNAAGPHSFIINRMAGIEEGMRIKTRALRHEVHHVPSPPGFNFEHDGYHTSDG
                     DNGIYFRPCVNNTILVGSEDPKCDPQEWIENPDVFNREVTDAQWAAQVSRLVRRFTNM
                     SMPEKKMGFAELYDVSDDWIPIYDKSDLDGFYLCIGTSGNQFKNGPVVGYCMSELIDR
                     CEKGQDHDADPVIVKAVYTGLELNMQQYSRRREINPESSFSVNG"
     gene            20512..21510
                     /locus_tag="PLS90_02035"
     CDS             20512..21510
                     /locus_tag="PLS90_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01213.1"
                     /translation="MIKCVSACLLSVCFVVSSTWAAAQLIWEKHPEPLIVPGDGNLPG
                     EDTVRSPSIVEMGDGTYRIYYGVGDLNGPGRVGGISMATAPVNDPRNLTQYPGNPIRT
                     GISGDGAFPFVVRLTDTQWRMYIGAGLYTSSDGINWIPYGTVLFPKAPENGIGSCCVI
                     FDGDHWKMYYTSIRLDAGLPDGREVHVALAVSDNGLNFQQIEANPLFPVRKNDNPDGY
                     EHINSKPFVLIDNGRYRMWYNEQTINRPYRIRYAESSDGVNWVRMFGSEGIDVSATGW
                     DAGMIEYPFVIRHGARYRMWYTGNHYGYDGYGLPTGIGLAEARLETRITPRNWLFY"
     gene            complement(21587..23191)
                     /locus_tag="PLS90_02040"
     CDS             complement(21587..23191)
                     /locus_tag="PLS90_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01214.1"
                     /translation="MDKQRILIRRGTILDGTGAAPRDADLLLENGVIAAIGALDGVGE
                     VAETIDAAGSYVAPGFIDIHSHSDFTLLVDPRAVSSISQGVTTEIVGNCGHGCAPIHN
                     PISAIANIYGYQAGYDLFWRSMAEYLESLEQARPALNVATLVPNGNLRQAVCGMSDRP
                     AGREEIARMTRLLEQGLEEGAIGYSTGLEYATEQGCSEEEVQALCRATGRRGGFYATH
                     TRNRPGEAVETIAEAIRAAQATGVSLQISHLASVARLDEQSTWAVEQAIEQVERARAA
                     GLRVHIDMHTRSFGTTNLSSILPPWALQGDQEAIAARLRDPAARDRMRAHPSIITSQA
                     RGDWSRIILFRCRSHPEFSQQSVQDIATRQGKEPFDAIFDLLLDEIDDLHQMMVISFA
                     YQEDDLHLPFGCPYCMAGSDAIALAPDGPLAGEVFHGAYTWAGYFYRTFVREKQWMTP
                     QEAIHRMTGLPAEKVGLKGRGRIEVGARADLAIFDPESFSDRGTTFEPNQLAVGMRHV
                     FVNGVPAVREGKITGLRAGQVIRSVC"
     gene            complement(23261..23734)
                     /locus_tag="PLS90_02045"
     CDS             complement(23261..23734)
                     /locus_tag="PLS90_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPK01215.1"
                     /translation="MTEVYQNIIGTLDREQVDYEVIQHEDVWTCDKMAEYCGCREAEI
                     LKSAVMQDQDGKMWVYVLPGDKKIKANALRRHLGVKSLSFAKKEAAEAACGCEIGCIP
                     PFGHQTALPIIIDEDVFAHERGYLSPGRHDCSIRIPNRFLRALPGARFYRTETAD"
     gene            24015..25406
                     /locus_tag="PLS90_02050"
     CDS             24015..25406
                     /locus_tag="PLS90_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01216.1"
                     /translation="MTPCRISLLGLCLDLYHTMIPEYIPVYQENLERYGAILGQRGML
                     VSRRLCFRRDEVDAAVSDAEAADADVLVIVYLAYAPSMIALPRLVRTHLPLLIWNVQN
                     LAGITPDFSARQRIENHAIQGHQDLCNGLVRARRPFGLASGHNADPRHLAALDDWLAA
                     CRAVSLSRKLRVGLLGRPFPGMGDFGVDETLMALRWGPDVVDIPMADLAAHFEAIAPS
                     AVDAEIADDHARFDIDPAVEPEHHRYSARLALALRRLAAERNLHAFTTNFGSFVNCPS
                     IQTAPFLGINHLIAANHGYAGEGNVTIAALDALLGRLFGQSNFCEMFTPDYANDRLFF
                     YHMAEGNYAMARPAPPPRLIQVPFFPDSCRPYLTPVYRYEPGPSTFVNLTTDAEERFR
                     LIAFEADVTDDGLDEPMTAPYFFIRAAGRVGDLIDAYGMAGGTHHLCRIRGRRSDQLA
                     RIARMLGMDWVRL"
     gene            25403..26905
                     /locus_tag="PLS90_02055"
     CDS             25403..26905
                     /locus_tag="PLS90_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPK01217.1"
                     /translation="MTQSSVIGVDLGSTNVKALLVGARGHIVARAAVPCPVSRDPAGR
                     VTQAPALWLDALDAVVTQLRAAAGSLEPVAALALSCQADTLVACDATGRELAPAISWM
                     DQRAHAESRRLERLRPDWRALTGKPMSPYSPLAKILWLRAHEPDLAAQTRRWCQPADY
                     LAAALTGQWVSDLNSASFSHGFSLERGDWDPALLAELDLEGTLAPLRPSGQVLAPLRD
                     AWRRRLGLPAQTQFVLGGHDQACASLGIQPPGADELDLSAGTAWVLFRPVSSRSAGAS
                     ENLVIYRHAVPDRWALLAAFPGGGVLEAFLDVLGVSHAEAFGPPGGGLLATLAADDLR
                     DDLIVLPFLLGASSLDTDASARAAILGLRSHHRPRNLIAGLMRAVAFEARRYYELFQL
                     PGDPPRRIKMMGGASRGPLWPQLIADACNTTVVVLDEPDASALGAAFLAGCGAGLWSW
                     DGLPAYPHGIPVSPDPARTEAAARAFADYRDALVADRLRRARESPVPSAT"
     gene            complement(26923..27750)
                     /locus_tag="PLS90_02060"
     CDS             complement(26923..27750)
                     /locus_tag="PLS90_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK01218.1"
                     /translation="MLNARDNLKETYDRNGYVFPIRVFPDEEADRYRKCYLEYVQSIR
                     EELAELKPREEYRFFAEAHAYLPWVYEMATSTGVLDAVEAILGPDLMIWDSRWFAKPP
                     RSSSYVSWHQDGVYYGLTPPNVATAWIALTRSGGENGAMQVIPGSHRGPQLPHEDKPS
                     DTNILARGQEISVQIRREEAVTLELKPGEMSIHHVGIIHGSEANRSDAWRIGLAVRFI
                     TPDVVQNAEQSFAMLVRGEDRFGHFTLFDKPAAAEGAAMAEARVRIIDQVYGNLMRK"
     gene            complement(27828..28706)
                     /locus_tag="PLS90_02065"
     CDS             complement(27828..28706)
                     /locus_tag="PLS90_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK01219.1"
                     /translation="MAALQHAPASRAESNGDTPTSAALVGTFGTERDDFEIDGRRAMM
                     LKPIREAPDGSRPWIFCAPVFMGPHPTQPEVNPNLFEHPVDEGSLIAGRPKTPEEATH
                     RKLLQKLLESGFHVGGIEVGESMGNPEGVEQYEKYYREVVRRYDLNPKACLYVTSRGA
                     LMHYNWAAQHPDQVLCIGANQPVIDLALFPGMEEAAKAYGMPLEEFTAVYREHNPIDN
                     LALLAGRSIPIFHIVGEADALLPLEHQIEMRDRYVALGGDLRLLAKPGIPHGLLPELL
                     EDMRMYEFFMEQAGLQ"
     gene            complement(28999..30078)
                     /locus_tag="PLS90_02070"
     CDS             complement(28999..30078)
                     /locus_tag="PLS90_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HPK01220.1"
                     /translation="MFATNGGAPPAEFTTPALLLDLDAAGRNIRAMAEFTNGKVKLRP
                     HAKTHKSPRIARLQMEAGAIGITCATLKEAEVLQAAGIESLLLANQVIEREPIARLIE
                     LARRGEVIVAIDSVENARAIAAAARRAACVVDCLVEVDVGLGRCGVAPGTAVVELARA
                     IAALEGLRYRGIMGYEGGMFLADPREKGRKCRECYRRLALTVELLRENQLPAEIVSSG
                     GTNTFREAAESGMMTEIQPGSYVTMDEHNRRFGLAFEYALKVVATVLSCPEPGRCVIN
                     AGKKAVSCDEGLPVCVDPALDLFKLNEEHGHVRYDGDRARLKPGDRIEIIPSHGCTTI
                     PLHEAYVLCRGGCVVGVEPIAARGH"
     gene            complement(30088..31203)
                     /locus_tag="PLS90_02075"
     CDS             complement(30088..31203)
                     /locus_tag="PLS90_02075"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01221.1"
                     /translation="MDEIPLHHVYTYNAVDRAFWREHLEDWLPRRIVDAHFHFIDPCY
                     QIETITEEMRRQYWVLETNLMPTAEVAEHCVRTTFPGREVQVLAFGFPTLGWDIEASN
                     EYVRRECLRRGWRAMALTRPTWVAEQVAWLLDQPGVIGIKPFYSMIGYSRQLREGHLE
                     ADILDFLPHHQLEVINERRAWVVLHVPKADRLGHPSNQRQIREIRRLYPDARLVIAHL
                     GRCYTRPHAEEGLLPLADDPGLYFDNSAVINPDVHELALRAIGPERIVYGSDNPVFYM
                     RGRRQWRGRTYINRTSHPFHFNTEREPPEVEARYTLYMYEALRGIKTACERLGLGRDA
                     VEAIFHGNAERIVADIESRKPAAASPVHAIAHSTEAG"
     gene            31516..32475
                     /locus_tag="PLS90_02080"
     CDS             31516..32475
                     /locus_tag="PLS90_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPK01222.1"
                     /translation="MPSLLARLRESDDVLIHYAPLHSMLSEDWGKPMEVHLSQWILEH
                     PDRFQDALARSYAAGCDFGSTSTQSSSAWRANLFGLKDQVRRLNYESARLAREVTPPD
                     KYLVGFCSTTLPEFLQPAGTLTPDQVRDGYREQMEALLEGGIDVFMIVGNQPDINLLA
                     IEVARDLAPDIPIISHCVYYHGPRGFFTMMGQTPREGARMLWDAGADVVGASCGLMRK
                     TDDYPTPGETHYYEGATELLGLLRQAGDGPLSIQPNASLSRLAEGKTVYAATPDEMLR
                     EVPNWIDRGARIVGGCCGTNLDHIRAIAPLIRRHNARTPSPRP"
BASE COUNT         5418 a        11589 c         9982 g         5841 t
ORIGIN      
        1 cagtggacga tgctccaggc gatgacggag gagccgcggc cgagcggcat tcccgaggtt
       61 gcccgaccga tcacgtacga tccgaccaac ggcaccgtga gcgcggggaa gcctactgcg
      121 cgacgtagat gtcccccgtg ctcacggtgc cgttggtcgg atcgtacgtg atcagtcggg
      181 caacctcggc aatgccgctc ggccgcggct cctccgtcat cgcctggagc atcgtccact
      241 ggccgttatt gacgatccac gggaagttgc tttcgtaatc cggccccgcg cagatcagca
      301 ggtagttggt ttgcctcttg gcgccggcgt cccaatccag ccgcagcgcc ggcgcctccg
      361 gtagatcggg atacgcgaac gatcctttga atggattctt cgccatcgac ttgaagttga
      421 cgtaggcaaa gcccagccgg ttcagattgc cgcccccgta gcgattatgt tcggtgaacg
      481 tatcgagcgg caggtccgtc atgtaggcga tcggcgtggt caattcccag gcgccgatat
      541 cgcgcatgtt ggtccggtcg tatccgcgca ggaaattgca gtaggtataa agatacagga
      601 aggaatagat cggcagcgcg cgcgggtact cattgttgtc gactacgtac gactccagcg
      661 cggtcttcac cagcgcaagt cggcctgcgc gcgggccacc tttgaacgcg tctgcgcttc
      721 caggaaattc gggacggcga tggcggccag gatcgcaatg atcgcgacaa cgatcagcag
      781 ttcgatcagg gtgaaggcga gtcgatcgcg ccgggcgcgc tgcaggagtc caatccgcgt
      841 ttccatgctg acgtgtctcc tctcgagtgg ggggtgggct cattcatggg tcaagggggc
      901 ggagcgctcg agttcgcctt catcccgcga cccgttgaat cattcaaaga tattccaacg
      961 cgaggcgtcg ttgaccgttt ctcccagccg gaaatcggga tcgctcgtca gggcgatcca
     1021 gtccagctcc acgaccgtat cggtggggtt gaattcattg atccatttgt cgcgcatgaa
     1081 ggtcgggtcg cacacctgtt cgccgctgac gaaattccac atgccgtaca ggttcatcag
     1141 catcccccgc agcgtgccgg cgtaggtgct ggtccgcggc gactgggtca ggtcgaacac
     1201 ctgtcggctg gtgatgttgg tctgcaccgt cgtgatgcgc aggctgatcg tgaaccagtt
     1261 ctcggcttcc tcggtcacgg ggtagatgat tatgccgacc tcgccgccgg ccgaggccca
     1321 gccgaagcgc ccactgatga cgagatattg atattccgtc tcaacgttgg ccaggttcag
     1381 gacggtgggg gtcacgcccg acgtgaccag cgcgccgcgc agatcaccca ctttgatgcc
     1441 ggtcgaatgg atgtggctcg ggttgctcgt cggcaattga cccggcgtca gcgtcgagaa
     1501 ccgctgcgtg agcagccccc cctccacctc aaagtcggtg gcggggatgc cgatcagcgc
     1561 ctgttgtggg gagggcagcc agccttcggt caccagcagg tccccatcga tcgccccggc
     1621 cggcaacccc tcggtggagg tctccgtgcc gtcgaaatcc cagacgatcg tcccggcccg
     1681 tgccgcgggc aacagcgcca gcagcatgag ggcgcccaat gtgaactggc gcattccgtc
     1741 ctcctttctc aaatcaggtg cgggtgcggg ttggcggccg gcgaaccgaa tatcgggccg
     1801 gccgccagat ggacgaatgc gacgtcactc cagggtaaac ctggatagct ccaccgggac
     1861 gtaacttgct cggaagtcgg gatcatcggt cagcgcgacc cagtcgagct ccacccgcgt
     1921 cgtggccggg ttgaactcac tctcccattt cgctttcatg aacgtcggat cgactgccgc
     1981 ggtccacatc ccgtagaagt cgaacatgat gccctgcagc gtgccgctgt acgagccggt
     2041 gcggatcgac gtcgtcaggt cgaacacctg gcgtccgtag gtgttggtca ccacgttgga
     2101 cagacgcagg ctgttggtga accaggccgc cgcttccgtc gtgacgggga acaggatcat
     2161 gccgacatcg ccccccgccg gggcccactc atgaatcccc cccagcatca ggtagggata
     2221 gtcggtttca atgttggtca ggttgagcag cgtcggcgtc gtggcgccgg gatagacgcc
     2281 gcgcaggtcg ccgacgatga ccccggtgca gtggatgtag ctggcatcgg tcgtcgggac
     2341 ctcgccggtg ggcagctcgg cataggtctg gatcagccgc ccgttttcaa cgttgaaatt
     2401 cgaggcggac agccccacgt tgaactgcgt tggattgggc cggaagccct ccggcgtgtg
     2461 tgtgtcgcca tcgatcgcgc ccgcttccag gccggcggtc gctccatcaa aatcccagac
     2521 gatggtcccg gcttgcccga cggcggcact cagcgccagg agaatcatga ttccaagaca
     2581 actgaccatt cgcatcatct tcagtgctcc ttcttgcaag gggtggtcga gccgtcagaa
     2641 catcctggct cattgagccg aacgccatgc agggtcaggg tccggcgatc cgggcggacg
     2701 gtcctccttt cagttcagga tcggggcgac gtcgcgagtc acgaagctac gggcggcgtt
     2761 gccgttcccg acttcgattg agacttcagc aagcccggcg ggatgggccc cgcttacagc
     2821 cccagccggc tccgcccggt gctgtcaatc gccgagtccc tgaaaaaggt cttgtcttgg
     2881 cccagctttt tgtctagtct gttgatgtat atacaccagc gttgctgtat aggcaactgt
     2941 tttttgcgcc ctcagagctc tcggcatgac gatttccact ggtttttttg aggcggtcgg
     3001 cacgaccatc aatcggcgcg attgtcttct ggcgcgcgac gcgaatcatc ctggagcagt
     3061 tctgcggcat gaactcttat cagacagtgg agaaaacctt tcgcattctg gaattactcg
     3121 cgaccaacgc cccgttgggc gtgacggaga tttccattga gctcggcatc gaaaaaagca
     3181 gcgtcagccg cctgctcaag acgctcttcg gcctgggcta cgtcgtccag tcgggtcatc
     3241 gcggccgcta tcagctcggc ccgcgcctgc tctacctggg cgactgttat ctgcgggaga
     3301 accgcctgtt gaaggaagcc gaaccgatcc tccaggagtt ggcgttcgcc acgcgctcca
     3361 gcgcccatct ggcggtgcgg gtcggcgagc aactgatggt cctggccaag gagcccagcc
     3421 ccgaacggat ccaggtcgct tccagcatcg ggtcccccgt ttcgccgcac gcctcggccc
     3481 tcggcaaggt gctgctggcg ggcatggacg agaaggaacg ccagaggttc ctgcctgagg
     3541 agttgatccc cttcaccgag cacacgatcg tcgaccggcg gcggctgctc gaggcgctcg
     3601 acgaggtccg gcgcaatggc tacgcgctgg agaccagtga agaggtgatg ggcgtcggct
     3661 gcatcgccgc gccggtgcgc gacagtgccg atcgctggat cgcggccctg tcggtctccg
     3721 gtccgcttca cggcacggcg ttccgccagg acgcccgcag cattgagctg gtggtccaga
     3781 aggcgctggc gttgtcggcg ctgatgggct accaacccga cccgcaaacg cgggcgcgca
     3841 tggaactgct caacagatga ccagacggga gcaccccatc atgcagacct cgaccgggct
     3901 acggccgccg cgcaacgaca aaccgtccgc cgccggcgag ccgaccgccg ccgaaatcgc
     3961 ccgccgcgcc gcccagtgca tcgccggcgg cgccgtctcc ctcaaccgca aggtggaacc
     4021 cgagctgctc ttcgtgcgcg ccgccggcag ccgcctgttc gatgccgacg gcaggcaata
     4081 catcgactac cacgccgcct tcgcccccca tctgctcggc cacaatcatc ccgtcatcaa
     4141 cggggccgtc cgcgaggcga tggagcgcca atggtcgctg atgggcagcg gcaccacccc
     4201 ctgggaggtc cgcctggccg agctgctctg cgccgccgtc ccctcgctcg agaccgttca
     4261 gatcgccaat accggcagcg aggccacggc ccatgccgtc cggctcagcc gcgcctggac
     4321 cggccgcaac cacatcatcc tgacgctggg cggatacaac ggctggcaca acgaggtcgc
     4381 ccgcgccgtt tcgcccagcc tcgagcaggt cggcccgcgc gtctcccccg gcgagtaccg
     4441 tttcatcccg ctctcggccg gcatccccga gtccacccgc agcctggttc acctcgtcaa
     4501 cttcaatgac ctggcttccg tcgaatacgt catgcggcgc tatcccgtcg cctgcgtgat
     4561 gaccgaaccg gtcctgcaga acatcggcgt tgtcccgccg cgcgagggtt acctccaggg
     4621 gctgcgcgat ctgtgcgacg agcatggcgc gctgctcgtc ttcgacgagg tgaagaccgg
     4681 tttccgttcc gccctcggcg gcttccagtc gatctgcggc gtgctccccg atctctcgct
     4741 gttcggcaag gccgtggcca acggctttcc cttgggcgtg atcggcggtc ggcgcgacgt
     4801 gatgagcctg ttcggcgacc ccgatccggc ccgccgcgtg ctcatcgcgg ggacctacaa
     4861 cgcgcacccg ttcacgtcgg ccgccgccat cgccacgatc gaatacctcc gcgaccagcc
     4921 cgtctatgcc cagttggaag ccctgggcgc ccgcatgcag gccggtctgg aacggatctt
     4981 cgccgaacac ggcatcaccg cgacggtcag tcgcatcggt tcggccttct gcgtttactt
     5041 ttgcgatcat gttccggtgg attatcacga tctgctgctg caccatgatt tcactttcga
     5101 tatgcgttac cggcgcgagc tgatcgaccg cgggatctat cattttccga ttgcctgcaa
     5161 gcagagttcg atcagcgccg cgcacaccat cgaggacatc gaccagacgc tggaaatcac
     5221 gcgcgacgtg ctccgcgaaa tctgaaggag tggcgcatga ggtggattcg ctttggcgag
     5281 ccgggcgatg aacgacccgg cctcccgatc ggtgcatcga tacgcaagga cggttcaggg
     5341 ctttttcgcg gcgcggaccc cggctttctc caggacgacg gccggcgccc tcgggtcgtg
     5401 cgtgcgcgcc accgcgcgaa agcggcccgc gcgtgaaaat cacggccgtc accaccttcg
     5461 tctgtcatgc ccggatgcgc aactgggtct tcgtcaaggt gcagaccgac cagcccggcc
     5521 tccatggctg gggcgaagcc accctggagt ggcatacgcg cgaagtcgtc ggcgccgttg
     5581 aggatctggc cgagctgctc atcggccagg accccacccg catcgagcac ctctggcaga
     5641 cgatgtatcg ccagcatttc tggcatggcc acgggatcgt gcgctccacg gcgatcagcg
     5701 gcgtcgacat cgcgctgtgg gacattctgg gcaaactcca cggcacaccc tgtcaccggc
     5761 tctggggcgg cgccgtccgc gactacgtgc gcacctactg ccatctgggc ggtgggcgca
     5821 tggaggactt ctacgaaaca gcgccttccg atgccggccg cttcgccgac ctcgcccgcc
     5881 aggccgtcga ggagggcttc acggcgttca aggcgatggc cgtccccgag acgatgccga
     5941 tcgaggggcg ccgcgccgtc cgctacgccg aagcctgcgt cggcgcgatg cgcgccgccg
     6001 tcggcgagca gatcgacatc atgatcgact gccacgcccg cccctccccc cgcatggggc
     6061 acctcttcgc ccgcgcgctg gagcccttcg gcctctactt tctggaggag ccgtgctggc
     6121 ccgagcatct gaaggacctg gccgagatcc agcgcgccgt ggcgacaccc gtcgccaccg
     6181 gggagcggct gaccggcctg cacgccttcc gcgatctgct ggagttgcgc gcgtgcagca
     6241 tcgtgcagcc cgacatcact cactgcggcg gtctgaccgt cgcccgcaag atcatggcgc
     6301 tggccgaggc gcatcagctc gcggccgcgc cgcacaaccc ccaggggccc gtcagcacgg
     6361 ccgcctccct tgaactgggg tttgccagcc cgccctatgt gatctgcgag acggtccatg
     6421 ccgatgtccc ctggcgccac gatgtcgtga ccgaagcgca cacgatcgag cgcgagggcc
     6481 gcatcgtccg cccccaccag cggccggggc tcggcattga gatcaacgag cgggaagtcg
     6541 ctaaacaccc cttccagccc gaagtcctgc tgcgcacgtt tcacgccgac ggcagcgtgg
     6601 gagactggta atggccaacc tggccggcag cacggcaatc atcagcggcg gcctgggcga
     6661 gattgcccag gccatcgcgc tggccatggc gcgggccggg gcgaacgtcg ccctgggcga
     6721 catccgcgcc ccccaggacg ccgaaccgat cctggcccaa ttgcgcgcca gcggcgttcg
     6781 cgcccgctat gaccacgtcg atatcgccga accggccgcc gtggagcgct gggtccgcga
     6841 cgccgagacg gacctgggcg tgcccgatct ggtgatctcc aacgcggcga cctgcacggc
     6901 ggccagcgtt cgctcgatca cgccggaggc ctggaatcgc gagctcgaca tcaacctgaa
     6961 cggcgcgctc tacctgagcc agtacgccgc ccgccgcctc gtgaccgcca accgccccgg
     7021 ccggattgtc ttcatcagca gttgggccgc cgaaaaagcc catcccgaca tccccgccta
     7081 ctgcgtcagc aaggccgccc tgcgcatgct ttgcaaatgc ctggccctca acctggccgc
     7141 cgacggcatc ctggtcaacg aactggcgct ggggttcgtc aatgccgggc tctccggacg
     7201 cgctttcaga ctctaccccg aggtgcaggc gcgttccacc gcctgggtcc cgattcagca
     7261 cctgatcgaa ccggaggaag ttgccgccga agtgctgcac ctgtgcgacc cggcggtcca
     7321 ccatatgacc ggcagcgtcg tcctgatgga tggcgggttg tcgatgacca atatcagcca
     7381 cgccagccgc ggctacggaa aggactgacc gaccatggcg cagcaaccgc cggcggtgca
     7441 tcacgccggg ttccatgggc ggatcggcgt cgcgcgccgt gagatcacgc ccccggtggg
     7501 gatctatgcg cgctgctggg ccgccgcgcg ccacgatgtg gccgaaggta ttcaccgccc
     7561 gctgacgctg accgcgctgg cgctcgcaac cgcggaagac cccacccccc acgtgctgat
     7621 cacgctcgac gcctgttcct ggggcgctcc cgaggcggag tggggcgtcc gcggcgaagt
     7681 gatccgccgc ctgggcgtcg acccggcgcg cgtcatcgtc gccctggccc atacccattc
     7741 gggcggcccc gcctttctca gcccgagcat ggacggattc cccggcggcg agcacgcgga
     7801 aaccttctac cacggcattg tccaggcggc gggggaggcc gccgtcgccg cgcgcgacgc
     7861 gagcatcccc gccacgctgg aatgggaata cggccgctgt gcgttggcgg ccaaccgcga
     7921 cctgccgctg ccggccggct acgtctgcgg ctacaacccc gggatcgccg ccgacgacac
     7981 gctgctcgtc ggccgcgtca ccgacgccga ggggcgcatc ctcgccaccc tggtcaacta
     8041 cgcctgccat ccgacgacgc tggcctggga taaccggttg atctcgcccg attttcccgg
     8101 cgcgttgcgc gagaccgtcg aaggcgccac cgccggcgtc ccctgtctgt atctgcaggg
     8161 caatatgggc gacctcgccc cccgcgagca gtatcagggc gatccggcgc tcgccgaccg
     8221 ccatggccgg cggctgggct atgccgtcct ttcggtgctc gaggggatgt tgccggccgc
     8281 gtcccgcctc gaatatcagg gggcgcagcg ctcgggcacg gcgctggggg tgtgggcggc
     8341 cgtgcctcac gttcatcccg gcgatctggc atcgctgact tttgatctcg atctgccgct
     8401 gaaggacgat ttcccccgcg atgaggaact cgaacggctg caggcaacga ccaacgaccg
     8461 ggttgaagcc gaaaaactgc ggcggaaacg gacggtccgc cgctggatcg gcaacggcgc
     8521 ctcggcgcgc ctgagcggat gggtctggcg cctcggcgac gccctgctcg tcggcctgcc
     8581 caccgaacct tactcccagc tgcaggtgga gttgcgccgc cggtttccgt gccatccggt
     8641 ctgcgcgctg agtctggcca atggcagcat cggttaccag cccaccgcgg cgatgtatgg
     8701 aaccgacgtc taccaggtct ggcagacgcc gctggcgcgg ggcaacctgg agctcacgct
     8761 cgaggagtgc agccggcggt tccaggagtt gctggaaggc gagccggcgc gggccgagac
     8821 cgcgcggggc ggttcggcgg ggttctgatc atcggcgccg gcttcacgaa ccggcgccgt
     8881 ctccgatgcg gacgcggttg cgatttgatc cgaaattcga attgacattg gagccctgcc
     8941 caaggcagtc tgatgcatat aaagcatcta tgttgcagac tgagcaacaa cgccccagcc
     9001 ttgctcgcgt ttcattcact ggtttccaag acccgatacg gagtcgcacc atgaccgctc
     9061 ccatcacacc ggcccagatt cgtcagttcc acgacgacgg ctacctgatg ctgcccggcc
     9121 tgttctccca ggaagaaatg gacctgctgc tgtcggccgc ccgcaatgac cgggccctgg
     9181 aggagaacgc cgtcggtctg accgatggga ccggccgcgt cagcaagctc tcgctgtggt
     9241 atatcccgcg cgacgacatc tacggtctgt tcagccgcag cgcgcggatc gtcgacaccg
     9301 ccgagcaatt gctcggcgac accgtatatc actaccactc caagatgatg ctcaaggaac
     9361 cgcggaccgg cggcgcctgg gtctggcatc aggattacgg ttactggtac gaagccggtt
     9421 atctctatcc cgacatgctg agctgcttga tcgcgatcga tcgcgcctcg cgtgagaacg
     9481 gctgcctgca ggttctcaag ggctcccaca agattggacg gattgcgcac aagctggtcg
     9541 gcgatcagac cggcgccgat ctggaacggg tggagcaggc ccggaaagtg tgcgaactgg
     9601 tctactgcga aatggagccc ggaacggctc tgttcttcca cagcaacctg ctgcactgct
     9661 cggcggccaa cacctcggac cggtcgcgct ggtcgctgat ctcgtgctat acggccgccc
     9721 acaacctgcc ctacaaacag gtggatcacc cgggctatga gccgctggtc aaggtgcccg
     9781 attcggcgat tctggaggcg ggggggcgcg gcctgtcgga gcgcaccgaa tttctctcgc
     9841 ggcaaaagct ggagggctac aaccgttcgc gggcggccca ggcgaacaat taacgttgat
     9901 cccgtccgcg cggtctgaac catgcaactg cagcttctcg acattatcat tttcgcgggc
     9961 tatttcattg gaattatcgt gttcgcgatc gtcgtcgcgg cgcgctcccg ctcgcgcgac
    10021 gccgccagct atttcctcgc ctccaatcag cttccgtggt acgccgtcgg cgcctcgttc
    10081 atcgccgcga acatctccac cgagcatttc atcggcatgg tcggctggtc cttcctctac
    10141 gggatgtcga tcgccaactg gtgctggctc aatgccgtca ccttttccgc gctgatctgg
    10201 attttcctgc cgttctacat gcgcggcgcc atcgccacga tgcccgagtt cctcgagcgc
    10261 cgcttcaacc gcttctgccg ctacgcctat gccctgttga ccgtcatcgg cctcgtcatc
    10321 gccctgctgg ggggcgtctt ttttgccggc gccaaggcga tcaccgtctt cttccccgag
    10381 gtgtcgacga ccgcggcgat catcattctg gccatcgccg cgggaaccta caccatctac
    10441 ggcggcctgc tttcggcggt ctgggccgat ctgctgcaat acatcctgct gatgacgggc
    10501 ggcctcgtcg ccgcgattta cggcctgtat tacgccggcg ggctggacga gctgatgcaa
    10561 cacctgcccg agaagttcat catcctctat ccctcgaccc acgaaatgat tccctggacc
    10621 ggcctgctga tgggcatccc ctccgtcggc ctgtggtatt cctgcgccaa ccagttcatg
    10681 gtgcagcgtt gcctgggcgc gcgctcggaa tgggacgcgc gcatgggcgt cgtgatggcc
    10741 gggttcagcc aggccatcct gccgctgctg atcgtcatcc ccggcatcgc cgccttctat
    10801 ctgttccacg atcaactgag cgacggcgac cagtcctggc cgttcatggt gcgccagttc
    10861 ctgccggcgg gcctggtggg gctggtgctg gcgggcctga ccagcgcggt gatgtcgacc
    10921 ctcagcgcga tcaccaattc ctcggcgacg atcttcacgc tcgatctcta taagaacatc
    10981 gtccggccac acgccgacga ccgcgagatc catcgcgttg gccgcatcag cggcgccgcc
    11041 gccatgttca tcggcgtgat cgtcgccttc gtgatggcgc gcgccgaggg cgccaccgtc
    11101 ttcggcctga ttcagaccgt cttctactat ctcgccccgc cgatcgccgc ggtcttcctc
    11161 gtcggcatca tctggtggcg cgccaccccg gcggccgcca ccgccgcctt gactctcggc
    11221 ttcgccgtct atctgccgct cgttgtcttc gtgatcttcc cgcgcatccc gctgctcgcc
    11281 ccctacgaca atttcatgca ccacaccttt ggcgtctttc tcctgtcggt cctgacgatc
    11341 atcattgtct cgctctttac gaagcccaag ccgcgcgaag cgctcaaggg cgtcatctgg
    11401 acgcgcagcg ccctggcggt tcccgaaggc gagcgcaagc ggcacaccgg aatcaggagc
    11461 ctcggcttgt ggtggacgat tatggtcgtg ctgacgatcg gcctttacgc ctacacctac
    11521 tccgtcggca gcaactccga agcgctcgag gccgagcgcc tggcctacac gacgtcgagt
    11581 gggacggcgc cgcgcgtgca gcgcaagacc gaactgaaga atttcaacct ctggacgggc
    11641 gacgggcagg tgctgttcga gccgcagcgc gacggcgagc gcctgacgtt caccttgccg
    11701 atcacggagc cgggccgcta ccagctggat gcgctcgtga ccaagggccc cgcctacggc
    11761 cagtacgata tcgaagtcca gggccagccc gcggaaatcc aatacaatat caccgaacgc
    11821 tctggcgccg gccattactc cgtccgtcac cttgcggcga gcacgttcga tgcgcgtcat
    11881 ccggccgccg cggcgccgtc gggcgggtcc gcttcacaga ttgaaagcat cgccggtgag
    11941 catgtcgtgc agcgcatcag tctgggctcg ttcgcgtttg ataatcccga catcgccacc
    12001 gtttcgttcg tcgcgcgcca tgttgaaccg ggacacgcgc tgatcggcgt cgacctgatt
    12061 gcgctgactc gcacgggtga cctgcccgat ccgacaaagg agtaacacca tgcaggggca
    12121 atatttcagt cgcgtcttcg cccgcacgcc gacgcggctc tggatcaaca atccgaccgg
    12181 cgacgacttg acccgcgcgc tgaacgtcgg ctgcatcgcc ggcaccacga acccctccta
    12241 ctgcgccaag ttgatcgacc aggaacccga attcatcctg ccgatcatcg acgaggaggt
    12301 ccagcgccac gaggatgact cggtggcggc cgatcgggtc gtgcaggccg cgactcgacg
    12361 cttcatggat gcctttcgcc ccatccacgg agccagcggc ggcacgctcg gtttcgtcac
    12421 gatccaggac gatcccgccc gcgatcacag cgccgaacgg atcgtcgagg cggccctcgt
    12481 gcaccagcgg gtcggcccga actacatggc caaggtgccg gtgaccgagg cgggcatgga
    12541 ggcgatggat gaattgatca gccgccgcat cccggtctgc gccaccgagt gtttcggctt
    12601 ggatcaggcg atcacgatgt gcgagcgcta tgagcgcgcc gtcgcccgcg gcgggcatcg
    12661 ccccccgttc ttcatcacgc acatcacggg catcttcgac gacgagctga aggatttcgc
    12721 cgcccgccac aacgtccaga tcaagcctga aatcctcaac caggccggct gcctggtcgg
    12781 ccgcaagcag taccatgtgc tgcgggaacg gggattcccg gccgtgatgc tgggcggcgg
    12841 cgcgcgcggg ctgcatcact tcacggcgtt tgtcggcggc cagatgcaca tcaccctcaa
    12901 ctggagcacg atcgaggaac tgctggacgc cgacgaaccg ggcgattgtc gaatcgacga
    12961 cctgcccgcg gacgacgtcg tggcggagtt gtgcgacaag ctccccgatt tccggcgcgc
    13021 ctggcacgag ggatcgctcc gcccggagga gttcgaatcc ttcgcgccgc tgcagcgctt
    13081 ccgcaacaac ttcctcgccg gctacggacg tctgcgcgag gaagtggcgc gccggcgcgc
    13141 cgcggcgatg gcccgtccat gactcggacg ctccccaccc tctgggacca gatcggcgcg
    13201 cactggcccg cgaactcgca tgaccgcgcc tggccttacg gcgaactcgc cgaggcggct
    13261 catgcgttcc tcgcggggca cggcgtcaac ccggtcgggc tgagccacta cggcatcgtc
    13321 gtcgccgatc gcaacgcggc cctcgcccgc ctgaaccggc tggcgcaaga accctggcgg
    13381 ccgacaaccg cggcctgggt cgaggccttc aatgtccatg tcatgcgcgg cgatctgcaa
    13441 ggcgtggaac tcgagttcat cgagccggac ggccccagtt tcttcgccga tttcctggcc
    13501 gggcaagggg agcgcctgca gcacatctcc ttccgcgtgg acggcatcca cgaggccgtc
    13561 gcgcggttgc gcgatgcggc cgtcccgctc gtcaacgatc aggttcgcga gggttcgcac
    13621 ggctggatcg cgtttctccg gcccgacccg ttcgctcccc tctgcctcga actctgtcag
    13681 ccgaaatagg ggttcctcgc gcatgaacga cagccccagc tccgccccac gttcgcgccg
    13741 acaattcctc ggcgacgccg ccgcctgcgc gttcctgtcg ggcgccctgc tgcccgccgc
    13801 cggggccctg cttgctgctc ccgccgcgcg ggcgcaggcg gcctcctccc agtccgaccc
    13861 ggcgaataag atccgcatcg gcatcgtcgg gggcggcttc ggcgcctcct ttcactggca
    13921 tctcgatccg ggctgcattg ttcacgcggt cagcgacctg cgccccgacc gccgcgagat
    13981 cctgaagcag acctaccagt gcgacctggc gtatgattct ctcgaagagt tgatcctcga
    14041 tccccggatt gacgccgtgg cgatcttcac cggcgccccg gaccacgcgc gccacacgat
    14101 cgccgccatg gagcagggca agcacgtgat ctgcgccgtg ccggcgtgtc tcacactcga
    14161 ggaagccgag gccctgctcg acgcccggcg ccggaccggc cggcgctaca tgatggccga
    14221 gaccagctac tacaatccgc acgggatcgc catgcgcgag ctctatcgcg ccgacgcctt
    14281 cggcgatatc ttcttcagct cggtggaata ttaccatccg atgagcgatg aggagcgccg
    14341 gcagcactgg ttctacgaca accagcgcac gtggcgtcac ggcttcgccc cgatgctcta
    14401 cccgacccac gccacgagct acctggtcgc caccaccggc gagcgactca ccgaggtgtc
    14461 atgccgcggc tttctcgccc ccgcgatcga gggctacggc gttggccaga acgcttatga
    14521 caacccgttc aacgggcaag tggcgctgtt cacgaccgat cgcggccatg tctgccgctg
    14581 caacgtgatc tggaccggat cgatccccac ggccgagcgg gccgagctgc tcggcacgaa
    14641 gctgagctac ttcatgccga tcgacgcgct cgatcaaccg gcgcgcgagc tggcggcggg
    14701 cggggcgcgg ggcgtggcct tgcccgatta cagccagcgg cttcccgagc cgatgcgcgt
    14761 ctcctcgggt catgccaact cccacccctt catcacgcac gaattcatcg cggcattgct
    14821 tgaggatcgg gaacccgaga tcgacctgca tcggtccctg gcgatgactg ttccgggcat
    14881 cgtggccaac gcctcctccc ggcgcaacgg cgaacggatg acgattcccg actacggcgg
    14941 cgggtcggtt tgagcgccgc gaacgcaccc atccggttcg ggacggcccg cgacgcttcg
    15001 ggccggcgga cgcggcgccg cgggcgtctc gatagcgacg gcccatttcc ctggttgagg
    15061 ctggaatgaa tcgcaacggt aaactgcgga tcggcgccga cgtcggcgga acctttaccg
    15121 acatcgtcct gatggatggc gacggcgccg tctggacgcg caaggtcccc tccacgccgc
    15181 ccaactacga acagggcgtg ctggacggca tcgcggcgct gctgaagacc gtgcccaacg
    15241 cggccggcga gcgcgtcggc gaagtcgccc atggcacgac ggtcgccacc aatgcgattc
    15301 tggagcggcg cggcgcccgc acggcgctga tcacgaccga aggctttcgc gacgtcttcg
    15361 agatccggcg cctgcgcgtc ccgcagcttt tcaacctgtt cttcgaaaaa ccgccgacgc
    15421 tgatcgaacg cttcctgcgc ttcgaggtgg ccgagcgcgt cgccgccacg ggcgaaatcc
    15481 tcaagcccct cgacgaggac tccctcggcc tgctggtcga tcagctccgc cggcatcagg
    15541 tggcgtcggt ggccgtctgc ctgctccatt cctacgcctt ccccgaccat gaactgcgcg
    15601 tcggcgacta cctgcgccgg catctgccgg gcgtggacgt ctcgctttcg gtgcacgtcc
    15661 tgcgcgagcg caaggagtac gagcggacgg cgacgacggc ggtcaatgcc tacgtcctgc
    15721 cggtgatgcg gggctacctg tcggcgctga aggacgggct cgagcgccag cggatccggg
    15781 ccacgctgca gatcatgcag tcgtccggcg ggttgacgcc cgacgaggat gccgcgcggc
    15841 gtccggtcta catcctggag tcgggcccgg cggcgggggt cttggccgcg cgcttcatcg
    15901 cgcgtcatct gggcctgccg cgcgtgatca cgttcgacat gggcgggacg acggccaagg
    15961 cctcaatgat cgagcacggc ggcatctcct acagccccga gtatgaggtc ggctccagcc
    16021 tgtcgtgcag cagccggctg gtcggcggcg cgggcgaact gatccgcgtg ccgacgatcg
    16081 acatcgccga agtgggcgcc ggcggcggca gcatcgccta tctggaccgc gccggcggcc
    16141 tgcatgtcgg cccgcgcagc gccggcgccc tgccggggcc cgtctgctac cggcgcggcg
    16201 gcaccgcgcc gacgctgacc gacgccaacg ccgtgctggg ctacatccgg cccggccggc
    16261 tggccgaggg cgacgtcgac gtggacgtcg aagcggcgcg ccgcgcgatc cacgaccaga
    16321 tcgcccgccc cctcgggctc gacctgttcg aggcggcgga ggggatccac cgcatcgcca
    16381 atgccgtcac gatgcgggcg ctgcgcgagg tctcgaccca gcgcggccgc gatccccgtc
    16441 agtatgtgct gatcgccttc ggcggctcgg ggccgatcca ggccgccggc ctggcgcgcg
    16501 aattgcaggt gctcaaggtg atcattcccc cgctgccggg gctgttcagc gccgccggcc
    16561 tcctgttctc cgatgtcgaa caccacgata tcaggagctg cctgctctcg ggcgactcgc
    16621 tccgcgccga cgcgatcgag ccgattcgcc gcgagatgca gcgcgcgatg cgcgaacgct
    16681 ttcaacgcga gggatacgcg cccgaccgcg tcgtgctctc caccagcgcc gatgtgcgtt
    16741 acaaggggca gttctctgaa atccgcgtcc ccctggacga cgccgaaatc accgcccaga
    16801 cgctcgaaca cctgcgcacg gcgtttgagg ctgagcatca tcaactttac ggccacagcg
    16861 ccggcgccga gagtccgatc gaagtcgtgt cggtccgtct gatcggctcg gtccccgccg
    16921 agacggggcg cacggcgctc cgtccggcgc cccgcgccgg cgattccccc gccagccgcc
    16981 cggtctggtt cggcgcccgc tggggcgccg tcgagacccc cgtcgtcgat cgcgccgcga
    17041 tcggcgaaca cgcctgcgga cccctgctca tcgacgaata cgattcaacg atcgtcgtcc
    17101 cgcccgacat gacggtgacg ctggatgatc agatggacat tatcatggag gcctgccgtg
    17161 ggtgagttgg accccatcac gcgggaaatc attaagaatg ccctggccac cgccggcgat
    17221 gagatggcga tcacgctcta ccggaccgcc tactccacga tcgtgcgcga ctgcctggat
    17281 tattcgacgc aactgaccga cgccgacggc aacatgatcg ctcagggcgt gacgatcccg
    17341 ctccacatcg gctcggcccc cttcgcgatg aaatccctgt ttgagaagtt cggcgacgac
    17401 atcgagccgg gcgacctctt cattctgaac gacccgttcg acggcgggat gcacaccccc
    17461 gacatcttca tcgtccgccc catcttctgg gagaacgagc gcgtggcgtt cgccatcgcc
    17521 accgcgcatc acctggatgt cggcggccgc gtggcgggca gctccgcctg cgacaacacc
    17581 gagatctttc aggaaggcct gcgcatcccg tggctcaagc tctatcgccg cggccagccc
    17641 gacgcctcgc tctttgctct cctgcgggcc aatgtgcgcg tccccgaaat gaccctcggc
    17701 gacctgcagg cccagatcgc ggcctgcatg accggcgaac gccacatcca cgaaatcatc
    17761 ggccgctacg gtctggcgac gttccgcgaa tgcacccgct ggctcatcga ctacacggag
    17821 cggattctgc gcgccgagat cgccacctgg cccgacggcc agtacagctt caccgattat
    17881 ctggacagcg acggcgtcgg cgggccgccg gtccgcatcc aggtgacgct gacggtgaag
    17941 ggcgacgagc tgaccgccga cttcaccggc tccagcccgc aggtgcgcgg cgcgatcaat
    18001 agcacgctct cctatacggc ctcggtcacg gccgcctgcg ttcgctcgct gctcggcgag
    18061 gaattgccca acaccgccgg gatgttccgg ccgctgcgga tcatcgcccc cgaagggacc
    18121 gtcgtcaatg tcgtcatgcc cggcgcctcc tcgatgcgcg gcgtcaccgg cttccgcacg
    18181 gtcgacaccg tcttcggcgc cctcgcccag atcctgcccg gtcgggtgat ggccgcgggc
    18241 gaggggggca atacgctcgt cattttcggc ggtcagcgcg agaaccgcgc cccctatgtc
    18301 tactacgagc tgctgaccgg cgactggggc gggcgtcccg accgcgacgg ctgcgatgcc
    18361 ctctgcaatc ccgtcaacgt ggcctccaat atccccatcg aggaagccga gtgcgcctac
    18421 cccatccgca tcgagcatta cggtctggcg cgcgacagcg gcggcgccgg ccgcttccgc
    18481 ggcggcctgg cggtccagcg ccagtggcgg ttgctctccg gcgaggcctc gctcacgatc
    18541 cgctcggacc gccgcgacca cccgccctac ggcctgcacg gcggcctgcc gggacagcct
    18601 tcgatcaata ttctctaccg tgacggcgag cagacgattc tgccgacatt cagctccggc
    18661 tcgatccgcg ccggcgagca gctctatcac cgcatgccgg ggggcggcgg ctggggcgat
    18721 ccgctcgacc ggctgcccga ggcggtcgcc cacgacgtgc tgaacgacaa ggtctcccgc
    18781 gaagccgccc gcgtgctcta cggcgtcgcc gtcgatgaac agtgtcgcgt ggacgaacac
    18841 gaaacaaccc ggctgcgcga ggccctgcgg caacaacgca ccggcgccgc cgcgacaacg
    18901 tgacccgcgc gcgagtttgt aatcgcaatg ccaagtgttg tcattgaatc catggtgtaa
    18961 ttcagttgag gaggacgaaa atgagcaaat acgatgtgat catcatcggc gccggtatta
    19021 tgggcgccgc caccgcctat gagctggcca aaaagggcta caaggtcgtg gtcattgaaa
    19081 agaacgcgca ggccggcacc ggcagcacgg cgggctcctg cgcgatcgtg cgcgcccact
    19141 attccacgta tcagggcgtc gccatggctt acgagggctt tttctattgg cttaactggg
    19201 aggattatct gggtgtgaag gaccccgagg gaatggcccg ctacatgaac tgcggcagta
    19261 tcctgctgaa gaccaaggga cacaattacc gcaaggtgct ggagcatttc cgcgccgcgg
    19321 gcgtcaagca cgaggaatgg acgctggaac agttgcgcga gaacatgccg ctctacaacc
    19381 acaaggcgtg gtggccgccg agccgccccg aagacacccc cgactactgg gaccgcgaac
    19441 cgaccgacac catcgacggc gccgtcttca cgcccgaatc gggatatgtc gaccggcccg
    19501 acctcgccac gcgcaacatg atccacgcgg cgaaagccca cggcgccgag ctgatgaccg
    19561 gccgccgcgt cgtccagatc catcgcgagg gcaatcgcgt gcagggcgtt tcgctcgaca
    19621 atgccgagcg catcgacgcg ccgatcgtcg tcaatgccgc cgggccgcat tcattcatca
    19681 tcaaccggat ggcgggcatc gaggagggga tgcgcatcaa gacccgcgcc ctgcgccacg
    19741 aggtgcatca cgtgccttcg ccgcccggat tcaatttcga gcacgacggc tatcacacct
    19801 ccgacggcga caacggcatt tatttccggc cttgtgttaa caatacgatt ctcgtcggca
    19861 gcgaggatcc gaagtgcgat ccccaggaat ggatcgaaaa cccggacgtg ttcaaccgcg
    19921 aagtcaccga cgcgcagtgg gccgcgcagg tctcccgcct cgtccgccgg ttcaccaata
    19981 tgtcgatgcc tgagaaaaaa atggggttcg ccgagctcta cgacgtctcc gacgactgga
    20041 ttccgattta cgacaagtcc gatctggacg gcttctatct ctgcatcggc acgagcggca
    20101 accagttcaa gaacggaccg gtcgtcggct attgcatgtc ggagttgatt gaccgctgtg
    20161 aaaagggcca ggatcacgac gcggatcccg tcattgtcaa agcggtctac accgggcttg
    20221 aactgaacat gcagcagtac tcgcgccggc gcgaaatcaa tcccgaaagc agcttctccg
    20281 tcaacggctg agcagacttc cgtcggagta gtaacttcga tgcaccaggg agcgggctat
    20341 cgactgacgc gaccaccggc atgggctttt gtatcaggcc gcgctctgta atcggccaca
    20401 cccgcccgtg gaaacaaaac gggcgggtat cttgtcattt caccggaatc caaggcggaa
    20461 agtacatttc ttattgagtg ctgaagcggg gaacacccgg agcgaccaat catgatcaag
    20521 tgcgtttcag cttgcctgct cagcgtgtgc tttgttgtca gttccacctg ggctgcggcc
    20581 caattgatct gggagaagca tcccgagcca ttgattgttc ccggcgacgg caacctgccc
    20641 ggcgaagaca ccgtgcgttc gcccagcatc gttgaaatgg gggatggcac ctatcgcatc
    20701 tactacggcg tcggcgacct caatggcccc ggtcgtgtgg gcggaatcag catggccacg
    20761 gcgccggtga atgatccgcg caatctgacg caatatccgg gaaaccccat tcgtacggga
    20821 atcagcggcg atggcgcttt cccttttgtc gttcgcctca cggatactca gtggcggatg
    20881 tatatcgggg ctggcctgta taccagttcc gatggaatca actggattcc gtacggcacg
    20941 gtgttgtttc ccaaagcgcc agagaacggc atcgggtcct gctgtgtcat tttcgatggc
    21001 gaccattgga aaatgtatta taccagcatt cggctggatg ccggcctgcc ggacggtcgg
    21061 gaggtccatg ttgcactggc tgtgtcggac aatggactca atttccaaca gattgaagcg
    21121 aatccgcttt tcccggtccg caagaatgac aatccggacg gatacgagca tattaacagc
    21181 aagccctttg tgctgatcga caacggtcgc taccggatgt ggtataacga gcagaccatc
    21241 aaccggccgt accgcatccg ctatgccgag tcctcggacg gggtcaattg ggtgcggatg
    21301 ttcggatccg aggggattga tgtttcggcc accggctggg atgcgggcat gattgaatac
    21361 ccgttcgtca ttcgccatgg cgcccgttat cgcatgtggt ataccggcaa ccactacgga
    21421 tacgacggct atggcctgcc caccggaatc ggattggccg aggcacggct ggagacccgt
    21481 atcacgccgc ggaattggtt gttctattga gctttgcctg gccggggctc aggtgcttcg
    21541 acgccacgga acccatcatt caatacgatg cttgcggcgg tattggtcag cacacggagc
    21601 ggatcacctg ccccgcgcgc aagccggtga ttttcccctc gcgcacggcc gggacgccgt
    21661 tgacaaaaac atgacgcatg ccgaccgcca gttgattggg ttcaaatgtc gtgccgcgat
    21721 cgctgaagct ctccggatcg aaaatggcca ggtccgcgcg cgccccgact tcgatccggc
    21781 cgcgcccctt taacccgact ttctccgccg gcagcccggt catccgatgg atcgcttcct
    21841 gcggcgtcat ccattgcttt tcccgcacga atgtccgata gaaatacccc gcccaggtat
    21901 aggcgccgtg gaacacctcc ccggcgagcg gcccgtccgg cgccagcgcg atcgcgtcgg
    21961 accccgccat gcagtacggg cagccgaacg gcaggtgcag gtcgtcttcc tgatacgcaa
    22021 aactgatgac catcatctgg tgcaggtcgt cgatctcgtc cagcagcaga tcaaagatcg
    22081 cgtcgaacgg ttccttgccc tggcgggtcg caatatcctg gacgctctgc tgcgagaatt
    22141 cgggatgcga ccggcagcgg aacaggatga tccggctcca gtcgccgcgc gcctgcgacg
    22201 tgatgatgct cggatgcgcc cgcatccggt cgcgcgctgc cggatcgcgc agccgcgctg
    22261 cgatcgcctc ctgatccccc tgcagcgccc acggcggcag gatgctgctc agattggtgg
    22321 tcccgaacga ccgcgtgtgc atgtcgatgt ggacccgcag tccggcggcg cgcgcgcgct
    22381 cgacctgctc gatcgcctgt tcgaccgccc aggtgctctg ctcgtcgagc cgcgccaccg
    22441 aggcaagatg cgatatctgc agcgaaacgc cggtcgcctg cgccgcgcgg atggcctccg
    22501 cgatcgtctc gaccgcttcg cccggccggt tgcgcgtgtg cgtcgcgtag aacccgccgc
    22561 gccgccccgt cgcccggcac agcgcctgca cttcttcctc cgagcagccc tgctcggtgg
    22621 catactccag accggtggag tagccgatcg ccccctcctc cagcccctgc tcgagcagcc
    22681 tggtcatgcg cgcgatttcc tcgcgacccg ccgggcgatc cgacatgccg cagacggcct
    22741 ggcgcagatt gccgttgggc accagcgtcg ccacgttcaa cgcgggccgc gcctgctcca
    22801 gcgactccag atattcggcc atcgagcgcc agaaaagatc atatcccgct tgatacccgt
    22861 aaatgttggc gatggccgag atcggattat gaatcggcgc gcacccgtgt ccgcagttgc
    22921 cgacaatctc cgtcgtcacc ccctgactga tcgaactgac cgcccgcgga tcgaccagca
    22981 gcgtgaaatc cgaatggctg tggatatcaa tgaagccggg ggccacatac gaccccgcag
    23041 catcgatcgt ctcggcaacc tcccccacgc cgtccagcgc gccaatcgcg gcgattacgc
    23101 cgttctccag cagcagatcg gcgtcgcgag gcgccgcgcc cgtgccatcg aggatcgttc
    23161 cccggcggat caggatgcgt tgtttgtcca cgtggaactc ccccgtcgcg gcccgttctc
    23221 cgctggatcg cgtccgcggc gccaacggaa gccgctcccc tcagtcggcc gtctcggtcc
    23281 ggtaaaatcg ggcgccgggc agcgcgcgca gaaaacggtt cgggatccga atcgagcaat
    23341 catgccggcc ggggctcagg tagcctcgct cgtgcgcgaa gacatcctca tcgatgatga
    23401 tcggcagggc cgtctgatgc ccgaacggcg ggatgcagcc gatctcgcag ccgcaggccg
    23461 cctcggccgc ttccttcttg gcgaaactca ggctcttcac ccccaggtgg cgccgcagcg
    23521 cgttggcctt gatcttcttg tcgccgggca gcacatacac ccacatcttg ccgtcctgat
    23581 cctgcatcac ggcgctcttg agaatttcgg cctcgcggca gccgcagtat tccgccatct
    23641 tgtcgcaggt ccagacgtcc tcgtgctgga tcacctcgta gtccacctgc tcgcggtcca
    23701 gcgtgccaat gatattctga tacacctcag tcatcgcgat gcggtccttt gggggagatg
    23761 gcggcggcgt ggaacggcct ggagcgggcg accagtttgc cagattgggc ggcgttcttc
    23821 caatgaaact cccggccgcg ccgtcctcaa aaccggccgg ctccggccga acgcccacgg
    23881 ccgcttcgcg cccgcccgtc ttggcttgat cttccccccg ccgcaagcta ttttcaccct
    23941 gttgcgtcct gaatcgcgga tcgctcctgg cccgcgtcgc ggcggcttcc aggaagggcg
    24001 ggatcctgcc tccgatgact ccctgccgca tcagcctgct cggtctctgc ctcgacctgt
    24061 atcacacgat gatcccggag tacattccgg tttatcagga gaatctggag cgctacggcg
    24121 cgattctcgg tcaacgcggg atgctggtct cgcggcggct gtgcttccgg cgcgatgagg
    24181 tcgacgcggc cgtctccgac gccgaggccg ccgacgccga tgtccttgtg attgtctatc
    24241 tggcttacgc gccgtcgatg atcgcgctgc cgcgcctcgt gcggacgcac ctccccctgc
    24301 tgatctggaa tgtccagaat ctggccggga tcacccccga cttcagcgcg cgtcaacgca
    24361 ttgaaaatca tgccatccag ggacatcagg atctctgcaa cgggcttgtc cgcgcgcgcc
    24421 ggccgttcgg cctcgcctcg gggcacaacg ccgacccgcg ccatctggcc gcgctcgacg
    24481 actggctcgc ggcctgccgc gcggtttcgc tcagccgcaa gctccgcgtc ggcttgctcg
    24541 gccgtccgtt ccccgggatg ggcgatttcg gcgtcgatga aacgctgatg gccctgcgct
    24601 gggggcccga cgtcgtcgac atcccgatgg ccgatctcgc cgcgcacttc gaggccatcg
    24661 cgccttcggc cgtcgacgcc gaaatcgccg acgatcacgc gcggttcgac atcgaccccg
    24721 ccgtcgagcc cgagcaccat cgctattccg cccgcctggc gctcgccttg cgccgcctcg
    24781 ccgcggagcg gaacctgcac gccttcacca ccaacttcgg ctcgttcgtc aactgcccct
    24841 cgattcaaac cgccccgttc ctcggcatca accacctgat cgccgcgaat cacggctacg
    24901 cgggcgaggg caacgtcacg atcgccgccc tcgacgccct tcttgggcgc ctgttcggcc
    24961 agagcaattt ctgcgaaatg ttcacccccg actacgccaa cgatcggctc ttcttctatc
    25021 acatggccga gggcaactac gccatggcgc gcccggcgcc gccgccgcgg ctgatccagg
    25081 tcccgttttt tccggattcc tgccgcccct acctcacgcc ggtttaccgc tatgagcctg
    25141 gcccctcgac tttcgtgaat ctgacgaccg atgccgagga gcggtttcgc ctgatcgcct
    25201 tcgaagccga cgtgaccgac gacggcctgg atgagccgat gaccgccccg tatttcttca
    25261 tccgcgccgc gggccgcgtc ggcgacctga tcgatgccta cggcatggcg ggcggcaccc
    25321 accatctctg ccgcatccgc gggcgccggt ccgatcagct cgcccgcatc gcccgcatgc
    25381 tcggcatgga ctgggtccgg ttatgacgca gtcttccgtg atcggcgtcg atctcggttc
    25441 cactaacgtg aaggcgctgc tggttggcgc gcgcggccac atcgtcgccc gcgccgccgt
    25501 cccctgcccg gtcagccgcg atcccgcggg ccgcgtcacg caagccccgg cgctctggct
    25561 cgatgccctc gacgccgtcg tcacccaact gcgggctgcc gccggttctc tggaaccggt
    25621 cgccgcgctg gccctctcgt gccaggccga taccctcgtc gcgtgcgacg cgacgggccg
    25681 cgaactggct ccggccatca gttggatgga tcagcgcgcc cacgcggaat cacgccggct
    25741 ggaacggctg cgcccggact ggcgcgccct gaccggcaag ccgatgtcgc cctacagccc
    25801 gctcgcgaaa atcctctggc tgcgcgcgca cgagccggac ctggccgcgc aaacccgccg
    25861 ctggtgccag ccggccgact acctcgccgc cgccttgacc ggccaatggg tcagcgacct
    25921 gaacagcgct tccttctcgc acggtttcag cctggaacgg ggcgattggg atcccgcgct
    25981 cctcgccgaa ctcgatctcg agggaacgct ggccccgctc cggccctcgg gccaggtgct
    26041 ggcgccgctg cgcgatgcct ggcgccgccg gctcggcctc ccggcgcaaa ctcaattcgt
    26101 cctgggcggg cacgatcagg cctgcgcctc gctgggcatt caaccccccg gcgccgatga
    26161 gctggacctc tcggcgggca ccgcctgggt cctcttccgc cctgtatcct cccgctccgc
    26221 cggcgccagc gaaaacctcg tgatctatcg gcatgccgtg cccgaccgtt gggccctgct
    26281 ggcggccttc cccggcggcg gcgtgctcga ggcgttcctc gacgtgctgg gcgtttccca
    26341 cgccgaggcg ttcggcccgc ccggcggcgg gctgctggcc actctcgcgg cggacgacct
    26401 ccgcgacgac ctgatcgtcc tgccgttcct cctgggcgct tcgtccctgg ataccgacgc
    26461 gtcggcgcgc gcggccatcc tcggcctgcg gtcgcatcat cgcccgcgca acctgatcgc
    26521 cgggctgatg cgggccgtcg cctttgaagc ccgccgttat tatgaattat tccagttgcc
    26581 aggtgatccg ccccgccgga tcaagatgat ggggggcgcc agccgcggcc cgttgtggcc
    26641 gcagcttatc gccgacgcct gcaacaccac ggtcgtggtt ctggatgaac cggacgcctc
    26701 cgccctgggc gccgcgttcc tcgcgggatg cggcgccggt ctctggtcct gggacggtct
    26761 gcccgcgtat ccccacggga ttccggtttc acccgatccc gcccgaacgg aagccgcggc
    26821 cagagccttc gcggattacc gggatgcgct ggtcgccgac cgcctgcgcc gtgcgcgcga
    26881 atcgccggtg ccgtccgcga cttgacgccc gatccgtagc cttcatttcc tcatcagatt
    26941 tccatagacc tgatcgatga tccgcacgcg tgcttccgcc atcgccgcgc cctcggcggc
    27001 cgccggcttg tcgaacagcg tgaaatggcc gaaccgatcc tcgccgcgga cgagcatggc
    27061 aaacgactgc tccgcgttct gaacgacgtc cggcgtaatg aaccgcaccg ccaggccaat
    27121 acgccatgcg tcggagcggt tcgcctccga tccatggatg atgccgacat gatggatcga
    27181 catctccccc ggcttcagtt ccagggtcac ggcctcctcc cgccggatct ggacgctgat
    27241 ctcctgcccg cgcgccagaa tgttcgtgtc ggagggcttg tcctcgtgcg gaagctgcgg
    27301 cccgcggtga ctccccggga tcacctgcat cgccccattc tcgcccccgc tgcgtgtcag
    27361 cgcgatccac gccgtcgcca catttggcgg cgtcagcccg taatagaccc catcctgatg
    27421 ccacgagaca tacgacgacg accgcggcgg cttggcaaac cagcgcgagt cccagatcat
    27481 cagatcgggg ccgaggatcg cttccaccgc atccagcacg ccggtcgacg tcgccatttc
    27541 atagacccag ggaagatacg cgtgcgcctc ggcgaagaag cggtattcct cgcgcggctt
    27601 cagttcggcg agttcctctc gaatcgattg cacgtactca aggtaacatt tccggtagcg
    27661 atcggcctct tcatccggga agacccgaat cgggaacacg tagccgttcc ggtcatacgt
    27721 ttcttttaga ttgtcacggg cattcagcaa ggggaggctc cgctgctttt atacattaaa
    27781 ataattccgg tcgaatccat tgggattcat cgccatcgaa tggcccgcta ttgcagtccg
    27841 gcctgttcca tgaagaactc atacatccgc atatcctcca gcagttccgg cagcaggccg
    27901 tgcggaatcc ccggtttggc caataggcgc aggtcgcccc ccagcgcgac ataccggtcc
    27961 cgcatctcaa tttgatgctc cagcggcaat aatgcgtcgg cctcccccac aatatgaaag
    28021 atcgggatgc tccggccggc cagcagcgcc aggttgtcga tcggattatg ttcgcggtaa
    28081 accgccgtga attcctccag cggcatgccg taggccttgg ccgcctcctc cattcccgga
    28141 aagagcgcca gatcgataac cggctgattc gcgccgatgc acaacacctg atcggggtgc
    28201 tgggccgccc aattgtaatg catcagcgcc ccgcggctcg tcacatacag acaggccttt
    28261 ggattcaaat cgtaacgccg cacgacttcc ctgtagtatt tttcatactg ctcgaccccc
    28321 tcgggatttc ccatcgactc gccaacctca atcccaccca cgtggaaccc actctcgagc
    28381 agtttttgca gcaatttgcg gtgggttgcc tcctccggcg tcttcggccg gccagcgatc
    28441 agcgacccct catccacggg atgctcgaac aggttcggat ttacctccgg ttgcgtcggg
    28501 tgcggtccca tgaacaccgg cgcgcagaaa atccacggac gcgaaccatc gggcgcttcg
    28561 cggatcggct tcagcatcat cgcgcggcgc ccatcaatct cgaaatcatc ccgctccgtg
    28621 ccgaacgttc cgaccagcgc ggccgacgtt ggcgtgtctc cattgctctc cgccctcgat
    28681 gccggcgcgt gctgcagcgc cgccatgagc atcagtcccg ccgcgacgag tcggccccgt
    28741 cggcgcctcg tgcccggccg ggtgtgtttc gccgaaccag caagtttccc ggcgaagccc
    28801 agcgctcgca gtgcggccgc ccattctgct cgccgccgcg caggatgcgt atcgccaatc
    28861 acaatgacaa tcgcggagcg aagcatgatc gtcgaacccc ttttaattct aattgaccaa
    28921 attccaacgg cgacgttccg gaccgatcca cgggcgggat gccttcggta ctccattcag
    28981 tgctacaccg tttcaacttc aatgaccccg cgccgcaatc ggctccacac cgacgacgca
    29041 ccccccacgg caaagcacgt aggcctcatg cagcggaatc gtcgtgcagc catggctggg
    29101 gatgatttcg atgcgatcgc ccggcttcag cctggcccga tcgccgtcat aacgcacatg
    29161 cccgtgctct tcgttgagtt tgaacaaatc gagcgcggga tccacgcaaa ccgggagccc
    29221 ctcgtcgcac gagaccgcct tcttgccggc attgatcacg cagcgccccg gttcagggca
    29281 gctcagcacc gtggcgacca ccttcagcgc gtattcaaac gccaacccga accgccgatt
    29341 gtgttcatcc atcgtcacat acgacccggg ttgaatttcc gtcatcatcc cgctctccgc
    29401 cgcctcacgg aacgtgttcg ttccccccga cgacacaatc tcggcgggta gctggttttc
    29461 gcgcagcagt tcaacggtca gcgcaagccg ccgataacac tcgcggcact tgcgcccctt
    29521 ctcccgggga tcggccagaa acatcccccc ttcatacccc atgattccgc gatagcgcag
    29581 gccctccagc gccgcaatcg cccgggccag ctctacgacc gccgtccccg gcgccacgcc
    29641 gcaccggccc agacccacgt cgacctccac caggcagtcg acgacgcacg cggcccgccg
    29701 cgccgcggcc gcgatcgccc gcgcgttctc aacgctgtcg atcgccacga tcacttcccc
    29761 gcgccgcgcc agctcgatca gccgcgcgat cggctcccgt tcgatgacct ggttcgccag
    29821 cagaaggctc tcgatcccgg ccgcctgcag cacctccgct tccttcaacg tcgcgcacgt
    29881 gatgccgatc gcgcccgcct ccatctgcag ccgcgcgatg cgcggcgact tgtgcgtctt
    29941 cgcgtgcgga cgcagcttca ccttcccgtt cgtgaattcg gccatcgcgc ggatgttccg
    30001 ccccgccgcg tccaggtcca gcagcagcgc gggcgtcgtg aattcggccg gcggcgctcc
    30061 gccgttcgtg gcgaacatgc ccgggcgcta tcctgcttcc gtggagtgcg caatcgcatg
    30121 caccgggctc gcggccgccg gcttgcgcga ttcaatatcg gcgacgatcc gctcggcatt
    30181 gccgtgaaag atcgcttcaa ccgcgtcccg ccccagcccg agccgctcgc acgccgtctt
    30241 gatcccccgc agcgcctcat acatgtagag cgtataccgg gcctccacct cgggcggctc
    30301 gcgctcggtg ttgaagtgga acggatggct cgtccggttg atatacgtcc ggccccgcca
    30361 ctgccgccgg ccgcgcatgt agaacaccgg attgtccgag ccgtagacga tccgttcggg
    30421 gccgatcgcc cgcagggcca gctcgtgcac atcggggttg atcaccgccg aattgtcgaa
    30481 gtacaggccg ggatcatccg ccagcggcaa cagcccttcc tcggcgtgcg gcctggtgta
    30541 acatcggccc agatgggcaa tcaccagccg cgcgtccgga tacagccgcc gaatctcgcg
    30601 gatttgccgt tgattgctcg ggtgccccag ccggtccgct ttcggcacgt gcagcaccac
    30661 ccacgcccgg cgctcgttga tcacctcaag ctgatgatgc ggcagaaaat ccaggatgtc
    30721 cgcctcgagg tggccctcgc gcaactggcg gctgtagccg atcatggaat aaaacggctt
    30781 gatcccaatg acgcccggct gatcgagcag ccaggcgacc tgctccgcca cccacgtcgg
    30841 ccgcgtcagc gccatcgcgc gccatccccg ccgcaggcac tcgcgccgca cgtactcgtt
    30901 gctcgcctcg atgtcccagc ccagcgtcgg gaagccgaac gccagcacct gcacctcccg
    30961 gccgggaaac gtggttctga cgcaatgctc ggccacttcg gccgtcggca tcaggttcgt
    31021 ttccagcacc cagtactggc gccgcatctc ctccgtgatg gtttcaattt gataacatgg
    31081 gtcgataaag tgaaagtgcg cgtccacgat ccgccgcggc agccaatcct ccagatgctc
    31141 gcgccagaac gcgcggtcca ccgcgttata cgtgtagacg tggtgcagcg ggatttcgtc
    31201 cattcagggg ctccggcggg cggtgcaaac gaaggtacgg cccctcccct gttttcgcaa
    31261 gcgaaacgtc gccgccgccg gccgccatag cctcggcgct cgccaaccac gcccccgcaa
    31321 tccgtccgat ccctcggatc cgacgaacaa ccaacaaaac cgacctgcat atccgcgatt
    31381 gacaatcaag cccaacactg gcaccctgcc cggtgggttg gacttgaccg ccccgccaat
    31441 cagaccgccc ccctccgccg gaccagcttg cagcggtttc agcctccgcg gagatcctct
    31501 ctcggatccg tcaccatgcc ttccctcctc gctcgtctgc gtgaatcgga tgacgtcctg
    31561 attcattacg ccccgctgca ctcgatgctc agcgaggact ggggcaagcc catggaagtc
    31621 cacctttcgc agtggattct cgagcatccc gaccgctttc aggacgccct cgccaggtcc
    31681 tacgcggccg gctgcgattt cggctctact tccacccagt cttcatccgc ctggcgcgcc
    31741 aacctttttg gcctgaagga ccaggtccgc cgcctcaact acgagtccgc ccggctcgcg
    31801 cgcgaagtca ctccccccga caaatacctc gtcggcttct gttccaccac gctccccgag
    31861 ttcctccagc ccgccggcac gctcaccccg gaccaggtcc gcgacggcta ccgcgagcag
    31921 atggaggccc tgctcgaggg cggcatcgat gtcttcatga tcgtcggcaa ccagcccgac
    31981 atcaatctgc tcgccatcga ggtcgcccgc gacctcgccc ccgacatccc gatcatctcc
    32041 cactgcgtct actatcatgg ccccaggggc ttcttcacga tgatgggcca gacgccccgg
    32101 gagggtgccc gcatgctctg ggacgccggg gccgacgtcg tcggcgccag ctgcggcctg
    32161 atgcgcaaga ccgatgacta ccccaccccc ggcgagaccc actactacga gggcgccacc
    32221 gaactgctcg gcctgctgcg ccaggccggc gacggtccgc tctcgatcca gcccaacgcc
    32281 tcgctctcgc gcctggccga agggaaaacc gtctacgccg ccacgcccga cgagatgctg
    32341 cgcgaagtcc ccaactggat cgaccgcggc gcgcgcatcg tcggcggctg ctgcggcacc
    32401 aatctggatc acatccgcgc catcgccccc ctgatccgcc ggcacaacgc ccgcaccccc
    32461 tccccccgac cctgaacgat cccatcgccg gcggcggggt cttcctcaat gcaaatcggc
    32521 ggcgccgtgt tcctgaaagt tcgccgacat gtcttttcgt gaaccttgaa tttggcggcg
    32581 gcatctcttc gtgcgagctc atcccgaccc cctcaacgcc accctgaaag cataaaaacg
    32641 gcctccctcc atacttcctt tacgtccctt atgtccctct ggggatcatc agcgacgtag
    32701 cacagccgat cattgaggcc cgttcagctt tttatcgcca ggatgtcgct cacctccgcg
    32761 gcgatcgttt ccagtacatc cagaccctcc gccagcgcat cctccccgat gacgtagggc
    32821 ggacagagct
//
LOCUS       DAOPCO010000013        32121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1546_length_32121_cov_5.308146, whole genome shotgun sequence.
ACCESSION   DAOPCO010000013 DAOPCO010000000
VERSION     DAOPCO010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 32121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32121
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1546_length_32121_cov_5.308146"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            267..1199
                     /locus_tag="PLS90_02085"
     CDS             267..1199
                     /locus_tag="PLS90_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01223.1"
                     /translation="MKQKARQRRRGASSGSLRPAHNRAEERGLIDRRREAGQRIRQQG
                     ITDTFHNGENPAMVQARWFISFFVLGVLCAVSVSAGTIVWDFDGPGTSLPAGVVDGDE
                     VDSWRQNPGAADLGLGAFTLESGKLIQTYDSLAEGALPYDGTAGGIHGTGIQVGNFGF
                     DWNGTGGALNLADVVTNYPYFIIGGKFGWSTDTTIGGVFYCVFDGAPWFANNLTFSTI
                     TTNVQGRHVVDMTAITPRQGAYAGELDAILWDVYALWFSGLVSPSEMATQWPAEFDND
                     PQVTYSSIDWIALTDDADFRSDYVPVSISLFELQ"
     gene            1335..2033
                     /locus_tag="PLS90_02090"
     CDS             1335..2033
                     /locus_tag="PLS90_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01224.1"
                     /translation="MKKGFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVARIQADL
                     RSVKTGLEAYRIDGNAYPRALPIYPFQYLYTFCNYLRGYDQVNHREIGAWELTTPIAY
                     ITSLPIDVFTQNNQFSAGDLSRLGFGYVNFESMAREPFRSSFAYPPEVGESPDLRLDW
                     EASSKKKSSYLLLSAGPDYNSNFPYIVNLGQWTMLHAMTDEPRPSGIPEIARPITYDP
                     TNGTVSTGDIYVTQ"
     gene            2150..2941
                     /locus_tag="PLS90_02095"
     CDS             2150..2941
                     /locus_tag="PLS90_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01225.1"
                     /translation="MKMNFSGNRWSAALAWMILLLGLPGGVRAGTIVWDFDTTDSTTI
                     NLEAGAIDGDALAPEWWRYINDGVGSYLIADEFVFEDGWFTLTFSAYEEGSMPFQNHG
                     SGIDVGSVISGGTELGLANVDTDYPYMIWYGELGWSTDNEIGSRFLFNVADRTWFGTQ
                     YNINFTPNERGRHVVDLSSVAQASGQAYGGTLNGFWWDVFGIWRTPTASEALPDFMAE
                     RWINEFDNDPSVTFLTIDWFALTDDPDFRSPGSQPTGALHWSFLN"
     gene            3029..5254
                     /locus_tag="PLS90_02100"
     CDS             3029..5254
                     /locus_tag="PLS90_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01226.1"
                     /translation="MHARRPRVPPRHLACLLVLGALGAMAPARAPGQPATPRQVINIG
                     GIDVALDGKTGGLLSLKDAGGTEFLRASAETAGMLSIDYPVKSFAGLRLEPKRSTASI
                     TREGEATTIAWAPLAASRPGYDLPAGQVLARVTLRPAPDGRSLILRAVVENESETTIG
                     KVLFPDLIGLRPIDAPERMELRMALGAVNPLAGPVFEPHHAPFYAPRMWHAYPAAGQY
                     QMNALRWMDYGSLKGGLSLHERRWLEEPRATILTHRSETDPDALRIAWQHEIHLPPNG
                     TWESAEFWLTPHRGGWAKGIEPYRDYVQRMNPEKNHPRAILEGLGMQTIWMIQTSEPE
                     AARADFTYADLLDVARDAKVHGLNLLVLWGWCTYGHLPMRHRPELGTEEELIEAIRKA
                     GELGVTIAPFINVKNLNDYLAPRYGVEPGNSAAWKFHPEMIPAMAPFFSDEPSGQIDI
                     ETTNTVWQQDVEETLVDWAERGAAHFCWDVFQDYGSMDLIHFIDRVRTAVRRVDPAAS
                     FGAEPYLGSFERATQVVDYTWNWVDYLEAGPYMNVLRWPRINCNVEASPLVVKMAFAD
                     GLFINAMPKRAGRPNGTALISEDAALAGALKEVANLRRQFQDYFTSGTFIGESVLAEP
                     VVEFVRRSNRSEIGGATFVAPAFEYPEIFVRGRLLDDRLLIIVLNLSDRPREVSFASD
                     LALWLPDADRLRVAHYDGRGELIADEELVWDRGSTWKGETGVLQPLEINLFEIAAESA
                     Q"
     gene            5464..7584
                     /locus_tag="PLS90_02105"
     CDS             5464..7584
                     /locus_tag="PLS90_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01227.1"
                     /translation="MEYNALRISLDDAHGSLRALEYPPVGTLLSAGAEAAGLLTVSIP
                     LPEFPGLQLETSGAAARIEAEDDYAAIRWEQLQFNRPELTSRLGGAVTAEARIEPAPD
                     GRSIILTATVHNQTEHPTSQILFPDLRGLRPHGDRELMELRMALGAVNPFAGPVRPEG
                     RSPFYAPVLWREFPAAAQYQMNALRWMDLGSLDGGFSLFERRWIEEPRPNILTHRNEA
                     DGDDLRIAWQHQIALGPGETWTSPPYWLTPHGGGWAKGIEPFREYVESVNPVLPISRP
                     ARVRDGLGFQTLWIMQRQEPNRAAFQFTDLIEIAEDAHAHGIDEVMLWGWNKGRTLPV
                     AINDKLGTVEDLIAAIGRAREIGVNLTMFTNVKNLDDSLAQRYGAVPSSSAAWTYHPE
                     MIPAMKPFFSDEPSGMFDIETTHATWQEDVRQTFREWAERGITSFGWDVFQDYGRMDL
                     ANLIAEIRKIIAPIDPQASFAGEPYLGSFERSNQVLDYTWNWVDYLEATAYMNVLRWP
                     RINCNIERSARVVKMAFAEGLYINAMPKQPNQPNGTRLISEEPELAAALKAVAPLRRQ
                     FLSYFTEGIPIGDSMLAKPVCRFVRQKQDSNLGGATAVLGPFEYPDIFVRAYQRPDRL
                     LLIVLQNESGAGPVSVTSDLALWLPAASEFEVLRYDTRGELLDRRAWRTDGVWEGVTP
                     PLDELEFCFFEIVAASPQSSQAEN"
     gene            7678..8727
                     /locus_tag="PLS90_02110"
     CDS             7678..8727
                     /locus_tag="PLS90_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01228.1"
                     /translation="MFPFARPILAQADDPCAPENSVLAQPVSWNIEEGRRVLAPGQLF
                     GEQDATRVAAGHVLRLGDRFRMYYWGAGNDGFNRICIAESPVDDPNAWEPVGIALERQ
                     PDSEYNSIGPGFPFVLPRDDGPWLMYYCGWGEPPEPGRVNNSTGVALSDDQGLTWRYH
                     ETHPVLPFDQPYDQQGTGSVWVVRSGDVYEMFYTSLSNYYAKPEGVVTGHGDVIPDIG
                     IGYARSTDGLVWEKPLKHLLLTPRRFGALPYEYIVSKPCIIRDGAGFRMWVNTFSDHY
                     RIRSLWSPNGKNWVWNASCAVGELGVGPEGAFDDEQRSYLTVVKDGDVYHGWYTGNQY
                     GLEGIGYLTGRVETQ"
     gene            8798..9802
                     /locus_tag="PLS90_02115"
     CDS             8798..9802
                     /locus_tag="PLS90_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01229.1"
                     /translation="MTPRHTARAKTFLLVVLLCACCAGSQAEEAGWHWRKHPEPVIRP
                     GDGGLPGENTVRAPCVVDLGNGTYRIYFGAGNLGDSNRGGICAAEAPIDNPTRWQVRP
                     EGALVEVDTSGAFPYVVRVQENEWRMYIGANLYLSEDGLNWERYADNPIVEQGESEDH
                     VASCCVLKVGPRWHMYYTRVLLDDSLPGGRRISVGLATSEDGIHFVKHPDNPLLDPRG
                     AGVHGEDYENINSKPYVLPGAGCYHLWYSEHCVQRPYRIRYARSSDGLHWERWAGLEG
                     IDVSPAGWDSQMIEYAFVLPHGDGYRMWYTGNQYGHNGEGPSGIGYAEAAAPQPPDAD
                     "
     gene            10049..12082
                     /locus_tag="PLS90_02120"
     CDS             10049..12082
                     /locus_tag="PLS90_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01230.1"
                     /translation="MDLTINGLDLSLDDETGGITRIASAATGELLLAPGNEASLLSMA
                     FPLPEFAALRLASRHSRAEIRETDDGVTIAWPTLGASRDTFPLPEGAVRAEATLRAAA
                     DGRSLIISCQITNDSTLDARNVIFPDLRGFLPFDGIEKTHLRLPLETIKPFSGPIQDP
                     EKSAFYTGRGWHDYPPRGLYGSNSLRWLDFGSLRGGISVFQRKWGTDDMPTIRTQRTE
                     AEPDRLRILWEHAVRIQPGESWQSGEFWLTPHAGGWAKGIEPFREYVRQVNPAVEAPS
                     QVRDDIGFQTIWMIQTVEKDPARAAFRFEDLVDVAREARDHGLHELCLWGFCNYSAMP
                     ITVRSELGTREEFLSAIAAARELGVNIAPFISIQVIHNDHVERYGVAPGRDDWTYHLE
                     LIPNFRPGYTDFWNGSIVPSSNEIWQSDVKAALGDWIEAGLCSFSWDVLETEYVDGKP
                     SPLLDLIDGIRQTARAKNPQSTFSAESVSPGSLEQMDRIVDYTWNWLDYTEAGPILNV
                     LPGLRVNCNVERSPLVVKKGFCDGLYLNVFPRKLDAPNGTARISEWPELSAALKEVAP
                     LRRQWLPYYTDGVFIGECVLSEPAEVHVAGHVLGDKMLVAVLNDSDTERSYSLSGVPW
                     LWLPADMAYNVRLTDSAGSVLEETVVEPGANWRTTERTMAPLELNFYEWQPAQ"
     gene            12130..12936
                     /locus_tag="PLS90_02125"
     CDS             12130..12936
                     /locus_tag="PLS90_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01231.1"
                     /translation="MRLPRWMLIALIGFIMVPAARAVENIRIHDFDIQLNSDTGGIIS
                     LSHDSVGLICQTAPADAGLLTLSYPFLQFTPMLLGAEYSDVAIESEGETIILRWSALG
                     PSRTHVPLPDGPVSGAVRLAPHADGKSLSLQAEVNNPTSFQVYQVLFPDLRGWRPMPS
                     AEDTRLQLPDQVLTPLVGPVLPPDRDPFYPPYIWKQFAFSGSARWVRYGNEGSGVRIE
                     QTSQPVNPVTQILTHRDEADPSYLRIVWQHSHRIEPGQNWQSGEFLITPY"
     gene            13073..16615
                     /locus_tag="PLS90_02130"
     CDS             13073..16615
                     /locus_tag="PLS90_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01232.1"
                     /translation="MNLNGLEITVDDQTGELQRLNYPPVGTILEAQAKLMAIHDGAGE
                     KGEEQTNPATGVASSVDASDATLATAEARRSDVTLNPREGGLTIGWSEAADAPSGAKD
                     AEKAVQYQIDLSAAPDGRSVILRGEVLNQTNQTLQQVAFPRLMGLRAFDEPQRMELRM
                     ALGAINPFAGDPFSIYYHPNGQYEMNALRWMDYGSLMGGISLFERHWLTEPRPSFFAT
                     RSAGQPDRFDLQWIHRVSVPAGQKWTSHEIWLTPHRGGWAKGIEPFRSYVRQVNPPRQ
                     VPDRVRDDVAFATFWMIEGNESDPLKAYFRYTDLENMALDCLQYGVREVVLWGSVGYG
                     TLPIPYAPMVGTQSQFANGVKAARQHGVNMVPFINVTSARILGKAELDRMLQDREQGF
                     LNGKSNAKAVWEWQPGFWTYHQEFIPAMIQGLIPAETLTLLSLPRGPSYSPEWQDAFR
                     RTLVDWAQKGVASFCYDVFEDDGTMQLTEMVQDIREELLQIDPESSFAGEPVNGSLER
                     AAQVLDYSWNWLDYIEAGPFHNVIRYPRFNCNVKDSVRVAKMGFADGLYLNIHPKRPN
                     QANGSKLISEEPALANALKVLSARREQFLPFFNRGIFIGESVLSAPVCDFVRQHKPSW
                     IGGAVVALGPFEYPEKFVRGHILDNRLLIIAMNNAAEPQAIELTTEPGMWIPAQNEYR
                     LTAYDENGQVLQESIVNANENGTVTLVSPELDPLELAFFVLEAPGRETTGIVWDFDGA
                     VGLTNMLEPGMVDGELALPIGPHDGMVAAGLDGEMDLADNWLTQTYETLSPGLLPQVA
                     HATAYQFGGEGPQATDFLLRNLAESYPYLVFYGDVGWSRNTSLGGVVTAVDPLRSAPL
                     DPATQTGIPSNQLRRHVIDLRVAAPTDWTGDAAQIIIDLFADWGDPARPDFMASEWLD
                     DFDPAPQASYFRCDWLALTNDPDFRLIHVNYPPKSSATPRAIVWDFDGPTSDLELNTV
                     DGDVVIPEGWRRFPINGDPLAGELRIEDGRLVHTFTGLPEGSLPTEIHMTGTAVGNFF
                     FFTPPGTALNLEDVAHNFPYLIFKGRIGWGANKPVGATIYANIDTESWFENRIEIAAI
                     RTNKEERQVLDLRQATQVTGQYDGTLIGLLIDWYALWYEGASPAFMASQWEQEFDSDP
                     EATFLSLDWIALSRNERFRETNNSAPAELWNLLK"
     gene            16694..17827
                     /locus_tag="PLS90_02135"
     CDS             16694..17827
                     /locus_tag="PLS90_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01233.1"
                     /translation="METIWIAENKLGTKLRRNRAFWRGELEEYPLLWFPVEGARPMPA
                     LPEIWPEERMWTDIEYVMESTENSLAGTYYGGDSLPIYCPWFGPDQVAAWLGAEIILK
                     PRENTSWVKPFVKDWEEHPELRIDPENELWKRYLEIYRAAVERGKGNWVTGYPDLHTG
                     IDALSAIRGPDHLSLDLLTNPGPIERAMIQMTRLWKEIVDTITDIILPGGQGTTNWTW
                     GWSDERYLCVGQNDYTCMISREMYDRFCLADNIECCRHVDISLYHLDGVGALHHLDTI
                     LGIEELDCVQWIWGNGKPPGSHWIDLLRQMQAAGKLVQVLYNADLLRSGIDIRHEIEV
                     LCRALDPSRLCIVAYLDDARLADHLVELAREICAAKRPTQIMM"
     gene            17886..19334
                     /locus_tag="PLS90_02140"
     CDS             17886..19334
                     /locus_tag="PLS90_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01234.1"
                     /translation="MKRRQFNAYLAAGLVLSHLPSATRRLIAASDPAAMKGGTGAGGP
                     LFELGGARHPHGQFTHVGLDEAGRLCLVKTDSRYAADGRYTSPVFEVPESQPVVIHAR
                     RRWTGPLRWERHQGNPVLSPSPEGWDQAQVTACSVVRVADRYRLYYGSRGAGIGFAEA
                     PVDEPHAWRKQERPCLTAGPPEAFDGKGVLSPEIVILDDGRWWMYYVGYHPEWEQNGT
                     AVHQLGLAESTDGGITWNRISPQPLLARGPAGSYDGFAVSSNSVWTDGRDWLMWYTGI
                     AQVPYLGSICLARSEDGRSWTRHEVNPVLPFNPHIAHEAFFVAKPHVVKDGNVFRMWY
                     SAVGYGDDCTQPGQYRVCYAESADGVQWERCEVNPVLRPAEAGWDSQMTEYAEVLHVE
                     HGWHMWYCGDGYGHIGYARGYPVAAAEIRARTGSIREPDDSWTPWIEVDAAPGAAGFQ
                     PTGAFMQIEVRLRTDDTAISPRVDFLRVMRDA"
     gene            19366..20490
                     /locus_tag="PLS90_02145"
     CDS             19366..20490
                     /locus_tag="PLS90_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK01235.1"
                     /translation="MITSRQRLLATIAGEPVDRIPVAPFIHSNYVHAVWSSAEVDLVE
                     KTAELYCDFGFDMIHRNCTPAYDDLLLEGENWTCQVELEQLGGNDTNRHYIIRTPEGE
                     LRQTIGTRWISKWECEVAPVDYLIKSEADLALLEKYQPPVGEIDGELIRRAARVVGED
                     GVVAPWINGVFNTVAYSYRPLGEVSLDPLLQPEFYDRMMRHFLGRCVAIARQFIAAGA
                     DFLSIGVNMASGKLFDGSFIEAYVLPYEMEFFAALREAGGATIAHNCGHASRLLPLYA
                     RLGTTIYESLTPPPHGDTRIEEAVRVLGPEVTLMGGLDQIDLLRHGSPAQVEQAVSQI
                     MQAVHSRCRYILGTSDYLLDETPPENLRALARAGHEMGVF"
     gene            complement(20833..21501)
                     /locus_tag="PLS90_02150"
     CDS             complement(20833..21501)
                     /locus_tag="PLS90_02150"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK01236.1"
                     /translation="MATKTSAPRAAKTQRSPRISNRDRILAAGIKLFAEHGPDATSVS
                     MISEASGLNRRMLYHYFGSKEGLYEEVLKAMYDRMNSVDVDLAHMLLPAEELLKKIIQ
                     GYYRFLNENPEFVRILSWENLRHGRTAKAIDVAAVKAPLIKALGVSLQMGARDGRFRS
                     DVDENQLLISCMALSFFFFSNQYTLSMLLGKDIHAPRLLEQRVNHVTQLILEGILIKQ
                     PTPA"
     gene            21765..22595
                     /locus_tag="PLS90_02155"
     CDS             21765..22595
                     /locus_tag="PLS90_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01237.1"
                     /translation="MTPSDTREAGTFCLRFDIDTNFTPWRRNNFCSRFGEDEPRFILR
                     LLDWLRERNCRAHFFAMGFGVIDYGESLRQIAQSEYPLDSHLHTHRVTLLDDYNAVYE
                     EMMTAHHLLASIGARIEGIGATGMYPEGIDGRPDIQELILQKGYRWVSSRWNLTRTLE
                     QMQPYRLPTGLLEIPCAGWSDMTFFHINEQRALAPEYQVERYELSTFTQHVKDMITRA
                     GREKLDYAICLHPAVLALHDPEMTFLEQVTAHARAEGVELVDLRQVHQRHAAVAAAAR
                     "
     gene            22885..24186
                     /locus_tag="PLS90_02160"
     CDS             22885..24186
                     /locus_tag="PLS90_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPK01238.1"
                     /translation="MAPRPDLAPAHGIDAGPGGRRAAALRISATGDPLAFGWWVRRLT
                     LKPGGWYRARVAFRTTDDVDPSLCVLVNLFWGEYPTRLKREEFFSHFSDAGDGWVFAE
                     HLFQMPDGEKQVDLQLHLHSAAAGSVWFANPAIEPAQAPPDRPVTLATTWFRRVDGSS
                     RAGMLKRIASRLDEAGHADADVVLIPECVTGHGVAGKDHRAQIEQDAEPLGGPATRLY
                     QDRARRHRMYVLGWIHERDGKRIFNTTLVIDRKGRIVGTYRKCHLHWPELWCGISPGH
                     ALPVFDTDFARIGIYTCYENWLPLVAQNLAMKGAEILFLPTAGYLVDVFRTRCLDNNC
                     YGVVAGLNSPSCIVASDGTLMAELRNDGLITARANLAVRPTHAYAYRHPNLTYGLPGA
                     DRLLDHSLSRMALDEISALTHGPIVGGPAPRPYVRDPGDER"
     gene            complement(24232..24672)
                     /locus_tag="PLS90_02165"
     CDS             complement(24232..24672)
                     /locus_tag="PLS90_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK01239.1"
                     /translation="MITGIHHVSFCVRDMDRSIAFYRDGLGFEPFSDRTIQGPVPEKV
                     IQLEGAHLRLVHLRGHGIGLELIQYFAPAGCPVAPRPCDVGSAHLCFVVDDIDAEMER
                     LERHGARFLSEAMTVEGGPNAGNRYVYFVDHDHIPMELSQPRKS"
     gene            24763..27450
                     /locus_tag="PLS90_02170"
     CDS             24763..27450
                     /locus_tag="PLS90_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK01240.1"
                     /translation="MWKQAVSAVRCVACIAALALAASSVSAADWPTFKHDRRLTGRTP
                     AKGAVTAEPEILGEYALTGVVTTLMADLNATSTSFQDVALPDEPALAGAQWTEPAPLI
                     DLRGDGVLVPPPGGPETLTGAVDQLVAQVLKDQPGYQSIKWHDYYSEEPTGYMEMLSY
                     EAGAEEPRQVWRSTPLEPGVYETLMLAVDANNDGQKDIALATHYHVAIWDGATGKKIM
                     EVRPPVARNYGSFGYFFVEGNPYPQFFTIVDFQSHFDVYGNDGKELTVLWREDVENPN
                     ILRVRKIVRVGPDPVADINGDGRLELVFNYYDRTGDREWHVMGYDALTGEIVLDLPET
                     FLDGIADITGDGFPEMFCKSTKGQKTKAPGELRIYSAAGGGDPAPIWRRPNSLWVTHD
                     APRRADLVSIGTDNRRTVMLANGPKPGLKSFFVTESAGERQTLRGFRLDEAREALPGL
                     QLELDGVKSLQISAIADVDQDGIDELKLECVGRDPVATAKANGRVVRLAMQETTGLTD
                     PPIVISRNEGPPLVAIPTMTREYVGLTFSEGQFHEQWRLPGWGMPGATFTQIGLTSAD
                     LDGDGAREILAAGKNERGEATVQASDLAGNPLWTQAFAEFDGDPPIWNVGGLTHIFAG
                     RFSDPQRDDVLVSVRRSIMHSDEGHMLDGATGEERWSADRVGIEAPGQGDTGYGFLGN
                     NIAAVDLNADERDEIVGVYPVDLYAFSGDGEQLMGRFLDGWGIYGQHFVHDFLGSGTP
                     QILSAAPGSVALLSSEGEIIWKDGEGEKAGTTWPLALTDMDGDGAMEASGAGFEDGFR
                     VYDGATGEVLHRMAVEKSTNRIVPAAGDLDGDGRDEVLWAVNQTLYAAGLKDGALGIE
                     WKKEFPALVRFPVLADITGDGLVEILVSCHNGRLYVLGN"
     gene            27486..28457
                     /locus_tag="PLS90_02175"
     CDS             27486..28457
                     /locus_tag="PLS90_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01241.1"
                     /translation="MNTWNDVVQPFLSSQALVWGPMVAAHQLRSRGWIRESIGKPMHT
                     VTFTLFGPLIYALGVWRLDLSFDRWYLVPIANAILLILSTLAAVILVPVLARSRQARG
                     TLSIMIPISNQGYTLAGFLTILFISDEAYGYNALLMIPAGFLLPLLWLPLAEHWGRGE
                     HTSIWVNYRRGMFSPMSLPLVGVIIGLLLNMLAVPMPAVAAGGLRWLVFLATANTMFA
                     LGACLHVRHLFGFFRTLRWVFLFKFVLHPALMFGLCLLLQLKGMAAAALFIASIMPAG
                     VMSVSLSSIYRLDLNLGNAGYLWTTLAFMIIVLPILIFLLRLPLFAY"
     gene            complement(28402..30048)
                     /locus_tag="PLS90_02180"
     CDS             complement(28402..30048)
                     /locus_tag="PLS90_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK01242.1"
                     /translation="MLPLRIRVVLQWILLLASAGLLAAEARRTTPRGQSAGGDPAAGP
                     EVISVWIGVIMGRWDEQATIKAVERFNRERDDIQIDLTLIPYTVYLAKLNLAISSGSP
                     PDVVNNAYNVVGNLPKREQLIELIVPIPESMVSPAMRAAWGPSIMRAITAEGLITQFP
                     SRTYLTGGVFVGNREHFELAGVDLRYYLEHGWDYETFKREMQKVQTAVRAKYGPEAYA
                     FGLNLLNVPTLLYECLLPPIVGPEVVERQFLLYDEAAGRYRLDPAITPETLAIPLRFM
                     QDLIHRDKLWGRKFLGLDFGQIVAELCERQELSATFTNTPGYSVSYQMLKQQEYDKGL
                     RERPLRTTNVPVPTPHGEDYVFGVGADGWGVMKQIPFRGDAHTSAALEVARYLASPEV
                     QAIYYVESKGMRLHLWPDAEAVEALTRDVPSVIDNDPWLTYIWELYEGWMKSARTYSN
                     MGGDWPDLKTRRDLLRIISGSSGGSYLFAGEGRQILERVLFNELEPLEAARMLLAGAA
                     ELIDDYYRRHPEAINRGAAAPGLNTRTAATAAERSESAAQ"
     gene            complement(30089..30955)
                     /locus_tag="PLS90_02185"
     CDS             complement(30089..30955)
                     /locus_tag="PLS90_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK01243.1"
                     /translation="MSSIKRDLRSLLGQGLAFSVLLFYTFLALLPLYLMIVSSLIPLG
                     VTFDVNDLQLYPRNPSLNNLFAFNHRVSGYLGRWLINSAIVSTLPVATNILFGVMAGY
                     ALGKMRFPGSRFLFWLMIATMTIPQFVILIPLYEMVWQFNWNDTYTALIVPFMAGIAT
                     VFLSRQFVQTLPGELLESATMDGCGEWGLFWHIILPLSRPLLGVLAIMGFVGAWLEYF
                     WTYLVIDSRAMYTVQMGILGVLGVDGGYAGELDYGEIMAASLVASIPVICVFFAAQRH
                     FVKGITFGGIKG"
     gene            complement(30942..31964)
                     /locus_tag="PLS90_02190"
     CDS             complement(30942..31964)
                     /locus_tag="PLS90_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK01244.1"
                     /translation="MNPVPYLALAMLVIGWATVPPSLCMASYLIRRKRAGGPAAVSAH
                     APTRLFSHWHAAYAFLAVAFGWSTLFLFYPLAKSLIYSFTDKSMDVQSGTQWVGLDNY
                     ARIIADPLWWKSVAVTSIYIAGTIPYSVLMSLFLASLIVSLPAFWQTFFKAALYLPGV
                     ISVVVSAAITKWIFYPGDGFANMLLTRLHLISENLTWFADPNLALPTLIVIGWLSTNG
                     IGVIIYCAALGNIPRSYYEAADIDGATAYHKFLRITWPLAKPSTVYVLITGMIGGFQI
                     FAPALLITGGGPQYTTYFVNYRIYQAFYHENQFGLACAMSVVLMAIIVLIAVINYRFL
                     ATDVEY"
BASE COUNT         5848 a         9915 c        10383 g         5975 t
ORIGIN      
        1 gttgtcgggt ctgatctggc cctgatgtta cgtccgatca aaaatgggca gcggcggcaa
       61 aaaacagttg ctcatgcagc aacactgatg tatatagtcc aatagatcga acacagatac
      121 tgctcgggaa gccaaggttt tcccctcctc acgccaccat gaaaggcaac gaacgccggg
      181 gatgggctgt ttcatcgaaa gcccttcagt tcaatccctg aagtcgtgcc cgggtggccg
      241 caggggtgat ccgggacccg gtcaacatga agcaaaaggc gaggcagcgg cgccgcgggg
      301 catccagcgg gagtttgcgt cccgctcaca atcgcgctga ggaaaggggg ctgatcgatc
      361 gccggcgcga agccgggcag agaattcgcc agcaaggcat tacggacaca ttccataacg
      421 gggagaatcc tgcgatggtt caggcaagat ggtttatctc attttttgtg ttgggtgttc
      481 tttgcgccgt gagcgtttcg gccggcacga tcgtctggga ttttgacggt cccggcacgt
      541 cgcttccggc cggggttgtt gacggcgatg aagttgattc ctggcggcag aatccgggcg
      601 ccgccgatct ggggctgggc gcgttcacgc tcgagagcgg gaagctgatc cagacatatg
      661 actcgctggc cgagggtgcg ctgccttacg atgggacagc aggcgggatc cacggcaccg
      721 ggattcaggt gggcaatttc gggttcgact ggaacgggac gggcggcgcg ctgaacctgg
      781 ccgacgtggt gaccaactat ccctatttca tcatcggcgg caaattcggc tggagcacgg
      841 acaccaccat tggcggcgtc ttctattgcg tgttcgacgg cgcgccgtgg tttgccaaca
      901 acctgacatt ttcgacgatc acgacgaacg tgcaggggcg ccacgtcgtc gacatgacgg
      961 cgatcacgcc gcgccagggc gcctacgccg gcgaactgga cgcgattctg tgggacgtct
     1021 acgcgctgtg gttttccggc ctggtctcgc cgagcgaaat ggccacgcaa tggccggccg
     1081 agttcgacaa cgatccccag gtgacatact cgtcgatcga ctggattgcg ttgaccgacg
     1141 atgccgattt ccggtcggat tacgtgccgg tgtcgatctc gctgtttgaa ctgcaataga
     1201 tccgggggca tggggctaga gtcgagtggg cgctcgccgg agaggaaacc ggcgcgtgcc
     1261 ccggtcgccc gaccgccgtg aagcccagga tggctcctca atacttcgga acagatcgaa
     1321 aggaagcaca acagatgaaa aagggtttta cgctgatcga gctgctgatt gtcgtggcca
     1381 tcatcgcgat cctggcggcg atcgcggtgc cgaacttcct cgaagcgcag acccgctcga
     1441 aggtcgcccg catccaggcg gatttgcgtt cggtcaagac cggactggaa gcctaccgga
     1501 tcgacggcaa tgcctacccg cgcgcgttgc cgatctatcc gttccagtat ctgtatacct
     1561 tctgcaacta cctgcgcggc tacgaccagg tgaatcatcg cgaaatcggg gcctgggagc
     1621 tgacgacgcc gattgcctac atcaccagct tgccgattga cgtcttcacc cagaataacc
     1681 agttcagtgc gggcgatctg agccgcctgg gtttcgggta tgtcaatttc gagtcgatgg
     1741 cgcgggagcc gttccgcagc tccttcgcct acccacccga agtcggcgaa tcgcccgacc
     1801 tgcggctgga ctgggaagca agcagcaaga aaaagtcgag ttatctgctg ctgtcggcgg
     1861 gtcccgatta caacagcaat ttcccgtaca ttgtaaatct gggccagtgg acgatgctcc
     1921 acgcgatgac cgatgagccg cggcccagcg gcatccccga gattgcccgt ccgatcacgt
     1981 acgatccgac caacggcacg gtcagcacgg gggacatcta cgtcacacag taagcaaaac
     2041 cgccccggcc gcccgcgttg acggccgggg cgccacaatc ctcgaatccg cattccccct
     2101 gacgacgctt cggcacggcc ccacactttg acgatcgcga ggtgacctga tgaagatgaa
     2161 ttttagcgga aatcgctgga gcgccgctct ggcctggatg attctcctgc tgggtctgcc
     2221 cggcggcgtg cgcgccggca ccattgtctg ggatttcgac acgaccgatt cgacaacgat
     2281 caacctggag gcaggcgcga tcgacggcga tgcgctggcg cccgaatggt ggcgttatat
     2341 caacgacggc gtcggttcgt atctgatcgc cgacgagttc gttttcgagg atggctggtt
     2401 cacgctgacg ttctcggcct acgaggaggg ttcgatgccg tttcaaaacc acggttcggg
     2461 gattgacgtg ggcagcgtga tcagcggcgg caccgaactg ggcctggcca atgtcgacac
     2521 ggactatccc tatatgatct ggtacggcga gctcggctgg agcacggaca atgaaatcgg
     2581 ctcgcgcttc ctgttcaacg tcgcggaccg gacatggttc ggcacgcaat acaacatcaa
     2641 cttcacgccc aacgagcggg ggcgacacgt cgtggatctg agttcggtcg cgcaggccag
     2701 cggccaggcc tacggcggca cgctcaacgg tttctggtgg gatgtcttcg gcatctggcg
     2761 cacgccgacg gccagcgagg cgctcccgga cttcatggcc gagcgctgga tcaatgagtt
     2821 cgacaacgac cccagcgtga cgttcctgac aatcgactgg tttgccctga ccgatgatcc
     2881 ggacttccgc agcccgggct cgcagccgac cggcgcgttg cattggtcct ttctgaactg
     2941 atcgcagccg cccggccctg gctggtccat ccccgcgcgc aggggtcacg cccgttctgc
     3001 gcgcgggcag tatttcaagg gatcgctgat gcacgctcga cgccccaggg ttccgccgcg
     3061 ccatcttgcc tgcctgctcg tgctcggcgc gctcggcgcg atggcgccgg cgcgcgcgcc
     3121 gggtcaaccg gccacccccc gccaggtcat caacatcggc ggaatcgatg tggcgctgga
     3181 cgggaagacg gggggcctgc tttcactgaa ggacgccggc ggcacggaat ttctgcgggc
     3241 ctcggcggag acggccggca tgctgagcat cgactatccc gtcaagtcgt ttgccggcct
     3301 gcggcttgag ccgaaacgat ccacggcaag catcacgcgg gagggagaag caacgacgat
     3361 cgcgtgggcg ccgctggcgg cgagccgccc cggatacgat ctgcccgccg gccaggtgct
     3421 ggcgcgggtg acgctgcgcc cggctcccga cggccgctcg ctgatcctgc gggccgtcgt
     3481 ggaaaatgag agtgagacga cgatcgggaa ggtgctgttt cccgatctga tcgggctgcg
     3541 gccgatcgat gcgccggagc ggatggaact gcgcatggcg ctgggcgcgg taaatcccct
     3601 ggccgggccg gtcttcgagc cccatcacgc gccgttttac gccccgcgca tgtggcacgc
     3661 ctatccggcc gccggtcagt atcagatgaa tgccctgcgc tggatggatt atgggtcgct
     3721 caaagggggc ctgagcctgc acgaacggcg ctggctggaa gagccgcggg cgacgatcct
     3781 gacccatcgc agtgaaaccg atcccgacgc gttgcgcatt gcatggcagc acgagatcca
     3841 tctgccgccg aacggaacct gggagagcgc ggagttctgg ctgacgccgc atcgcggcgg
     3901 gtgggccaag gggatcgagc cctatcgcga ttatgtccag cggatgaacc cggagaaaaa
     3961 ccatccgcgg gccattctgg agggcctcgg gatgcagacg atctggatga tccagacgtc
     4021 cgagccggag gcggcccgcg ccgacttcac ctatgccgat ctgctcgatg tcgctcgcga
     4081 tgcgaaggtc catgggctga atctgctggt cctgtggggc tggtgcacgt atggccattt
     4141 gccgatgcgg caccgtccgg agctgggaac cgaggaagaa ttgatcgagg cgatccgtaa
     4201 ggcgggggaa ctcggggtca ccatcgcgcc gttcattaat gttaagaatc tcaatgatta
     4261 tctggccccg cgctatggcg tcgagccggg caactcggcc gcgtggaaat ttcatcccga
     4321 gatgatcccc gcgatggcgc cgttcttcag tgacgagccc tccggtcaga tcgacattga
     4381 aacaaccaat acggtctggc agcaggacgt ggaagagacg ctggtcgact gggccgaacg
     4441 gggtgcggcg cacttctgct gggatgtgtt ccaggactac ggcagcatgg acctgattca
     4501 ttttatcgac cgcgtgcgga cggcggtccg ccgggtggat cccgcagcct cgttcggggc
     4561 cgagccgtat ctgggcagct ttgagcgcgc gacgcaggtg gtcgactata cctggaactg
     4621 ggtcgattac cttgaggcgg ggccgtatat gaacgtgctg cgctggccgc gcatcaactg
     4681 caatgtcgaa gcctcgccgc tggtggtgaa aatggcattc gccgacgggc tgttcatcaa
     4741 tgcaatgccg aaacgcgcgg gccggcccaa tggcacggcc ctgatttcgg aggacgcggc
     4801 gctggccggc gcgctgaagg aagtcgcgaa cctgcgccgc cagtttcagg actatttcac
     4861 gtcgggaacg tttattgggg aaagcgtgct ggccgaaccg gtcgtggaat tcgtccggcg
     4921 ttcgaatcgc agcgagatcg gcggcgcgac gtttgtcgca cccgcctttg aatatccgga
     4981 aatcttcgtc cgcgggcgtc tgctcgatga tcgcctgctg attattgtcc tgaatctgtc
     5041 ggaccggccg cgcgaggtgt cgttcgcctc cgatctggcg ctctggctgc cggacgccga
     5101 ccgccttcgc gtcgcgcatt atgacggccg gggcgagctt atagctgacg aagaactggt
     5161 gtgggatcgc ggatcgacct ggaaaggcga aacgggcgtt ctgcagccgc tcgaaatcaa
     5221 tctattcgaa attgccgccg aatccgcgca gtgaaccacc ggactccagt ggtcgggtca
     5281 gcatgtgcca gcggcacggg acgcattaac atgggccgag ccgaatcata ccccggatcg
     5341 accgccccga ttcacgcgcc ccgcatcgcc gggaggcgac ggagcgcggt cgcgcaaggc
     5401 cagctatggc ctctctggct tgcgagcctg atcctacttt ccgctgatcc aagcgccgtt
     5461 gagttggaat acaacgccct gcgcatctcg ctggatgacg cccacggctc gctgcgcgca
     5521 ctggaatatc ctccggtggg aacgctcctg agcgcgggcg cggaagcggc cggtctgttg
     5581 acggtcagca tcccgctgcc ggaattcccc ggactgcaac ttgagacgag cggggcggcc
     5641 gcgcggatcg aggcggagga cgattacgcg gcgatccgtt gggagcaatt gcaattcaat
     5701 cggccggagt tgacatcccg gctgggcggc gccgtgaccg cggaagcgcg gatcgagcct
     5761 gcccccgacg gccggtcgat catcctcacc gcgacggtcc acaaccagac cgagcatcca
     5821 acgtcgcaga tcctgtttcc cgatttgcgc ggactgcggc cgcacgggga tcgcgaactg
     5881 atggaactgc gcatggcgct gggcgcggtc aacccgttcg cgggtcccgt ccggccggag
     5941 gggcgcagcc cgttctatgc gccggtcctg tggcgtgaat tcccggcggc ggcgcaatac
     6001 cagatgaacg cgctgcgctg gatggacctc ggctcgctgg acggcggatt cagcctgttc
     6061 gagcgccgct ggattgagga gccgcgtccg aacattctga cgcatcgcaa cgaagccgat
     6121 ggcgacgacc tgcgcatcgc ctggcagcac cagatcgccc tcgggcccgg tgaaacgtgg
     6181 acgagcccgc cctactggct gaccccgcat ggcggcgggt gggccaaggg aatcgagccg
     6241 tttcgggaat atgtcgagag cgtgaatccc gttctcccga tcagccgccc ggcgcgcgtg
     6301 cgcgatggcc tggggttcca gacgctgtgg atcatgcagc ggcaggaacc gaatcgcgcc
     6361 gcgtttcaat tcacggacct gatcgaaatc gccgaggatg cgcacgcgca cggcatcgat
     6421 gaggtcatgc tctggggctg gaacaagggg cggacgctgc cggttgcgat caacgacaag
     6481 ctggggacgg tcgaggattt gatcgccgcg atcggccgcg cgcgcgaaat cggcgtgaat
     6541 ctgaccatgt tcaccaacgt taaaaatctg gacgacagcc tggcgcagcg ctacggcgcc
     6601 gttccgagca gttccgccgc ctggacgtat catcccgaga tgatccccgc gatgaagccg
     6661 ttcttcagcg atgaaccctc ggggatgttc gacatcgaaa cgacccatgc cacgtggcag
     6721 gaagatgtgc ggcagacgtt ccgcgagtgg gcggaacgcg gcattacttc ctttggctgg
     6781 gacgttttcc aggattatgg ccggatggat ctcgcgaatc taatcgccga aatccgcaag
     6841 atcatcgccc ccattgatcc gcaagccagc tttgccggcg agccgtatct ggggagtttc
     6901 gagcgatcca atcaggtgct ggactacacc tggaactggg tcgactacct ggaagcgacc
     6961 gcgtatatga acgtgctgcg gtggccgcgg atcaactgca acatcgagcg atcggcgcgg
     7021 gtcgtgaaga tggcattcgc ggaaggcctg tacattaatg cgatgccgaa gcaaccgaat
     7081 cagcccaacg ggacgcgact gatcagcgag gagcccgagc tggccgcggc gctgaaggcc
     7141 gtcgcgccgc tgcgccgaca gtttttatcc tatttcaccg agggaattcc cattggcgat
     7201 tcgatgctgg cgaagccggt ctgccgattc gtgcgtcaga agcaggacag caacctgggc
     7261 ggcgcgaccg ccgtgctcgg cccgtttgaa tatccggata tcttcgtgcg tgcttatcag
     7321 cgccctgatc ggctgttgtt gatcgttctg cagaatgaat ccggggcggg ccccgtcagc
     7381 gtcacgtcgg atctggcgct gtggcttccg gcggcgtcgg aattcgaggt gctgcgctac
     7441 gatacgcgcg gtgaattgct cgaccgccgg gcctggcgca ccgacggggt ctgggagggc
     7501 gtcacgccgc cgctggacga actggagttc tgtttctttg agatcgtcgc ggccagcccg
     7561 cagtccagtc aggccgaaaa ctgaaacgag gagactcgca tgaaaccacg tgcttccgcg
     7621 cccggagttt ttgcgttttt caggcttcgg ctgatctggc tgggattcgc ggtgttcatg
     7681 tttccattcg cccggccgat cctggcgcaa gcggatgatc cgtgcgcccc ggaaaacagc
     7741 gttctcgccc aacccgtgag ctggaatatc gaggagggcc ggcgcgtgct ggcgccgggg
     7801 cagcttttcg gcgagcagga cgccacgcgc gtcgccgccg ggcatgtcct tcgcctcggc
     7861 gatcgcttcc ggatgtatta ctggggggcc ggaaacgatg gcttcaaccg catctgcatc
     7921 gccgaaagcc ccgtcgacga tccgaacgcc tgggaaccgg tgggcatcgc gctggaacgg
     7981 caaccggaca gcgaatacaa ctcgatcggc cccggcttcc ccttcgtgct gccgcgcgac
     8041 gacgggccgt ggctgatgta ttactgcggc tggggcgagc cgcccgaacc gggacgggtc
     8101 aataattcaa ccggcgtcgc gctcagcgac gatcagggat tgacctggcg ctatcacgag
     8161 acgcaccccg tactgccgtt cgatcagccc tacgaccagc aggggacggg gagcgtctgg
     8221 gtcgtgcgca gcggggatgt gtatgagatg ttttatacct cgctgagcaa ttactacgcg
     8281 aaaccggaag gggtggtcac gggtcatggc gacgtgatcc ccgacatcgg catcggctat
     8341 gcgcggtcta ccgatgggct cgtctgggag aagccgctga agcatctgct gctgacgccg
     8401 aggcggttcg gggcgctgcc gtatgagtac atcgtctcca agccgtgcat tattcgcgac
     8461 ggcgctggat tcagaatgtg ggtcaacacg ttcagcgatc attatcgcat ccgcagtctg
     8521 tggagcccca atgggaagaa ctgggtctgg aacgcgagct gtgcggtcgg cgaactcggc
     8581 gtggggcccg agggagcgtt cgatgatgag cagcgcagct atcttacggt cgtgaaggac
     8641 ggcgacgttt atcatggctg gtacaccgga aaccagtacg ggctcgaggg aatcgggtat
     8701 ctgaccgggc gcgttgaaac ccaatagaaa ccagcgccgc gactttactt ttattattaa
     8761 taaagcatgc gctccggcga gttgaaaagg gaacatcatg acacctcggc ataccgcgcg
     8821 agcgaaaacg ttcctcctcg ttgtgctgct ctgcgcatgc tgcgccggca gccaggccga
     8881 ggaggccggc tggcactggc ggaagcatcc cgaaccggtc atccgccccg gcgacggcgg
     8941 gcttccgggc gaaaacaccg tgcgcgcccc ctgcgtggtc gatctcggga atggcacata
     9001 ccgcatatac ttcggcgcgg gcaatctggg cgattcgaac cgggggggga tctgcgccgc
     9061 cgaggcgccg atcgacaacc ccacccgctg gcaggtgcgc cccgaggggg cgctcgtcga
     9121 ggtcgatacg tccggggcct tcccctacgt cgtccgtgtc caggaaaatg aatggcgcat
     9181 gtatatcggc gccaatctgt acctgagcga ggatggcttg aactgggaac gctacgcgga
     9241 taatccgatc gtcgagcagg gcgaaagcga agaccacgtc gcctcctgct gcgtgctgaa
     9301 agtcggtccg cgctggcaca tgtactacac gcgcgtgctc ctggacgatt cgctgccggg
     9361 tggacggcgg atcagcgtcg gcctcgccac gtccgaagac gggatccatt tcgtcaaaca
     9421 cccggacaat ccgctgcttg acccgcgcgg cgcgggcgtc cacggggagg actacgagaa
     9481 catcaacagc aagccctacg tcctgcccgg ggcgggatgc taccatctct ggtacagtga
     9541 gcattgcgtc cagcgcccct accgcattcg atacgcgcgt tcgtccgatg ggctgcactg
     9601 ggagcgctgg gcggggcttg aggggatcga tgtatcgccc gcgggatggg actcgcaaat
     9661 gatcgagtat gcctttgtgc tgccgcacgg cgacggctac cggatgtggt acaccgggaa
     9721 tcaatatggt cacaacggcg aggggccgag cggcatcggg tatgccgagg ccgccgcgcc
     9781 gcagccgccg gatgcggatt gattcgcggc atgcggagtg acggcgtttc gagagccgag
     9841 ggcgagggac gagagccgag agccgaattt ttggggttac ctttggggac gatttcgagg
     9901 acgagggacg agggcgaggg acgacgttag actgaggagg agcgggaaca tgccctggat
     9961 caggcgttgg ttcgcaggct cgcgacatcc gcggtgcacc gcggtatttc ttgccgtttg
    10021 cctgctgagt ccgacttggc ttacggccat ggatcttacc atcaacggcc ttgacttgtc
    10081 gctggacgat gaaaccgggg gcatcacgcg catcgcttcg gcggcgacag gtgaactgct
    10141 gctggcgccg ggcaacgaag ccagcctgtt gagcatggcc tttccactgc cggaattcgc
    10201 ggcgctgcgg ttggccagcc ggcattccag ggcggagatc cgcgaaaccg acgacggcgt
    10261 gacgatcgcc tggccgacgc tgggggcgtc gcgcgacacg tttcccctcc ccgagggggc
    10321 ggtccgggcc gaggcgactc tccgcgccgc ggcggatggc cgctccctga ttatttcctg
    10381 tcagatcacg aatgattcga cgctcgacgc ccgcaatgtg attttcccgg atctgcgggg
    10441 atttctcccg ttcgacggca tcgagaagac tcacttgcgc ctcccgctcg aaacgatcaa
    10501 gccgttttcg ggacccattc aggatcccga aaaatccgcg ttctataccg ggcgcggctg
    10561 gcacgattat ccaccccgcg gcctgtacgg ctccaattcg ctgcgctggc tcgattttgg
    10621 cagcctgcgg ggcggaatca gcgtcttcca gcggaaatgg gggaccgacg acatgcccac
    10681 gatccggacc cagcggacgg aggccgaacc cgaccgcttg cggatcctgt gggaacatgc
    10741 cgtccgcatc caaccgggcg aaagctggca gagcggcgaa ttctggctga cgccccacgc
    10801 cggcggctgg gccaagggca tcgaaccctt ccgggaatac gtgcgccagg tgaatcccgc
    10861 cgtcgaggcg ccgtcccagg tgcgcgacga cattgggttt caaacgattt ggatgattca
    10921 gaccgttgag aaagatcccg cccgcgccgc atttcgtttc gaggatctcg tcgatgtcgc
    10981 gcgggaagcc cgcgaccatg ggctgcacga gctttgcctg tggggcttct gcaactattc
    11041 ggcgatgccg attaccgttc gttcggaact ggggacgcgc gaggaattcc tgagcgccat
    11101 cgccgccgcc cgcgagctcg gcgtcaacat cgcgcccttc atcagcattc aggtgattca
    11161 caatgaccac gtggagcgct atggcgtcgc gcccggccgg gatgattgga cgtatcatct
    11221 ggaattgatt ccgaattttc gccccggtta caccgatttc tggaacggct cgatcgtgcc
    11281 gagcagcaac gaaatctggc aaagcgacgt gaaggcggcg ctcggcgact ggatcgaagc
    11341 cgggctctgc tcattcagct gggacgtgct tgaaaccgaa tacgtggacg gcaagccgtc
    11401 gcccttgctc gatctgatcg acgggatccg gcagacggcg cgcgcgaaga atccgcagtc
    11461 cacgttctcc gccgaatccg tttcgccggg aagcctcgaa cagatggacc ggatcgtgga
    11521 ttacacctgg aactggcttg actacaccga ggcggggccg atcctgaacg tgctgccggg
    11581 tctgcgcgtc aactgcaacg tcgagcgctc gccgctggtt gtgaaaaagg gcttttgcga
    11641 tggcctgtat ctcaacgttt ttccgcgcaa gctcgacgcg ccgaacggca cggcacgcat
    11701 cagcgagtgg cccgaactct cggccgcgct caaggaggtg gcgccgctgc gccgacagtg
    11761 gctgccgtat tataccgacg gggtcttcat cggcgaatgc gtcctgagtg aaccggccga
    11821 ggtgcacgtg gccggccatg tcctgggcga taaaatgctg gtcgccgtgc tgaatgattc
    11881 ggacaccgaa cggagttatt cgctgtcggg cgtgccctgg ctctggctgc cggccgacat
    11941 ggcgtataac gttcgtctga ccgattccgc gggcagcgtc ctcgaggaga cggtcgtcga
    12001 gcccggcgca aactggagga ccaccgagcg cacaatggcg ccgctcgagc tgaacttcta
    12061 tgagtggcaa ccggctcaat gagtcggagc gccggggcgc gcgccccggc ccgaatgagg
    12121 aggtgaagca tgcgtttacc gcgatggatg ttgatcgcgt tgattgggtt catcatggtc
    12181 ccggcggccc gggccgtcga aaatatcagg atccatgact tcgacattca gctcaattcc
    12241 gataccggcg gcattatttc attgagtcac gattcggtcg ggctgatctg ccagaccgca
    12301 ccggccgacg ccggcctcct gacgctctcc tacccctttc tgcaattcac gccgatgctg
    12361 ctgggggctg aatactccga tgttgcgatc gagagcgagg gggaaacgat catcctgcga
    12421 tggagcgcgc taggccccag ccgcacgcat gtgccgttgc cggatgggcc ggtgagcggc
    12481 gccgtccgcc tggcgccgca cgccgacggc aaatcccttt cccttcaggc tgaagtcaac
    12541 aatccaacca gtttccaggt ctatcaggtc ctgttccccg atctgcgcgg gtggcggccg
    12601 atgccgtccg ccgaggatac ccgcctgcaa ctgccggacc aggtgctgac gccgcttgtc
    12661 ggccccgtcc tgccgcccga ccgcgacccg ttctaccctc cctatatatg gaagcagttc
    12721 gctttctcgg ggagcgcgcg ctgggtgcgc tatggcaatg agggatcggg cgttcgaatt
    12781 gagcagacga gtcaaccggt gaatcccgtc acgcagatcc tgacccatcg cgacgaagcg
    12841 gacccgagtt acttgcgcat cgtctggcaa cattctcatc ggatcgaacc gggtcagaat
    12901 tggcagagcg gagaatttct gattacgccc tattgaagcg acggactgtg acaagaagga
    12961 gaagtttcta atgggttttc ctcatcctgc gaaggtgagc cggcattcac gcgcattcct
    13021 tgtggtcgcg atcggaattc tattattcat tgaacacgca tttgcggtta cggtgaacct
    13081 gaatggcctc gagatcacgg tggacgacca gacgggtgag ttgcaacggc tgaactatcc
    13141 gccggtgggg accatcctcg aagcgcaagc gaagttgatg gcgattcacg acggcgccgg
    13201 cgaaaagggg gaggagcaga ccaaccccgc caccggcgtt gcatccagcg tcgacgcatc
    13261 ggacgcgaca ctggccacgg cggaagcgcg gcgaagcgac gtgacgctca atccacgcga
    13321 gggcggcttg accatcggct ggagtgaggc cgccgacgcg ccatccgggg ccaaggacgc
    13381 ggagaaggcg gtgcagtatc agatcgatct gagcgccgcg cccgacggtc gttccgtgat
    13441 ccttcgcggg gaagtgctca atcagacgaa tcagacgctg cagcaggtgg cgttcccgcg
    13501 cctgatgggg ctgcgggcct ttgatgaacc gcagcggatg gaattgcgca tggccctggg
    13561 cgcgatcaat cccttcgcgg gcgatccgtt ttccatttat tatcatccca atggccaata
    13621 tgaaatgaat gcgctccgct ggatggatta tggcagcctg atgggcggga tcagtctgtt
    13681 tgaacggcac tggctgaccg aaccgcgccc ctcctttttc gccacgcgga gcgcgggtca
    13741 gcccgatcga ttcgatctcc aatggattca tcgcgtctcg gtgccggccg gccagaaatg
    13801 gaccagccat gagatctggt tgacgcctca ccgcgggggc tgggccaagg ggatcgaacc
    13861 gttccgcagc tatgtccgcc aggtgaatcc gccgcgccag gtgcccgatc gcgtccgcga
    13921 cgatgtggcc ttcgccacgt tctggatgat cgaggggaat gaatcggatc cgttgaaagc
    13981 ctatttccgg tacaccgatc tggagaacat ggcgttggat tgtcttcaat acggcgtccg
    14041 tgaagtcgtc ctctggggga gcgtcggcta cgggactttg ccgattccct acgcccccat
    14101 ggtggggacc cagtcacagt tcgctaacgg cgtcaaggcg gcgcgccagc atggcgtcaa
    14161 catggttccg ttcattaacg tcaccagtgc acgcattctc ggcaaggccg aactggatag
    14221 gatgctgcag gatcgcgagc agggttttct gaacggaaaa agcaatgcca aagcggtgtg
    14281 ggaatggcaa cccggcttct ggacctatca tcaggaattc atcccggcga tgatccaggg
    14341 gttgatcccc gccgagacgc tgacgctgct ttcgctaccg cgcggtccgt cgtattcgcc
    14401 ggagtggcag gatgcgttcc gccggacgct tgtcgactgg gcccagaagg gcgtcgcgtc
    14461 gttctgttac gacgtgtttg aagatgacgg cacgatgcag ttgaccgaga tggtccagga
    14521 tatccgcgag gaattgcttc agatcgaccc cgaatcctcc ttcgcggggg agccggttaa
    14581 tggctcgctg gaacgggcgg cgcaggtact cgactactct tggaactggc tggattacat
    14641 tgaagccggt ccgttccata acgtcatccg ctatccgcgg ttcaattgca atgtgaagga
    14701 ttccgtccgg gtcgcgaaaa tgggttttgc cgacgggctc tatctcaaca tccatccgaa
    14761 gcgccccaat caggccaacg gttcgaagct gatcagcgag gaacccgcgc tcgcgaacgc
    14821 gctcaaggtg ctgtccgcgc gccgcgagca gtttctgccg ttctttaatc ggggcatttt
    14881 cattggcgaa tcggtcttga gcgcgccggt ctgcgacttt gtgcgccagc acaagccgag
    14941 ctggatcggc ggcgccgtcg tcgcgctcgg tccctttgaa tatccggaga agttcgtgcg
    15001 cggccatatc ctcgacaatc gcctgctgat cattgcgatg aacaatgctg ccgaaccgca
    15061 ggcgatcgag ctgacgacgg aacccggcat gtggatcccc gcgcaaaatg aataccggct
    15121 gaccgcctat gacgagaacg gccaggtctt gcaggaatca attgttaatg ccaatgaaaa
    15181 cggaaccgtg acgctcgtta gcccggagct cgacccgctg gagctggcct tcttcgttct
    15241 tgaggcgccc gggcgcgaga cgaccggcat cgtctgggac tttgatgggg cggtgggatt
    15301 aaccaatatg ctcgagccgg ggatggtcga cggagaactc gccttgccga tcggccccca
    15361 cgatggtatg gtcgccgcgg gactggatgg ggaaatggac ctcgccgaca actggttgac
    15421 gcaaacctac gaaacccttt cgccgggtct gcttccgcag gttgcgcacg ccacggccta
    15481 ccagttcggc ggggaagggc cacaagcaac ggatttcctg ttgcgcaatc tggcggaatc
    15541 ctacccgtat ctggtgttct acggcgatgt cggctggtcg cgcaatacct cgctcggcgg
    15601 cgtggtcacc gcggtcgatc cattgcgctc ggcgccgctc gacccggcca ctcagaccgg
    15661 gatcccgtcc aatcaattgc ggcgacatgt gattgacctg cgcgtcgcgg ctcccaccga
    15721 ttggaccggc gacgcggcgc agattatcat tgatctgttc gcggactggg gcgaccccgc
    15781 ccgcccggac ttcatggcga gcgaatggct cgatgacttt gatcccgcgc cgcaggcatc
    15841 ctattttcgc tgtgactggc tggccttgac gaatgacccc gatttccggc tgattcatgt
    15901 taactatccg ccgaagtcca gcgcgacgcc gcgcgcgatt gtctgggatt tcgatggccc
    15961 caccagcgat ctggagctga acaccgtcga tggggatgtc gtgattcccg agggttggcg
    16021 ccggtttccg attaacggcg atccgcttgc gggcgaactg cgcatcgagg acgggaggct
    16081 ggtgcatacc ttcaccggcc ttcccgaggg atcgctgccg acggaaattc acatgacggg
    16141 gacggcggtc ggcaattttt tctttttcac gcctccgggg acggcgctga atcttgagga
    16201 tgtcgcgcat aatttcccgt atctgatctt caaaggccgg atcggctggg gcgcgaacaa
    16261 accggttggc gcgacgatct acgccaacat cgatacggaa tcctggttcg agaatcggat
    16321 tgaaatcgcg gcaatccgca caaataagga agaacgccag gtccttgact tgcgccaggc
    16381 aacacaggtc accggacagt acgacggcac gctgatcggc ctgctgatcg attggtatgc
    16441 cctgtggtac gaaggggctt cgcccgcctt catggcgtcc caatgggagc aggaattcga
    16501 ttccgacccg gaagcgacgt tcctcagtct ggactggatc gcgctgagtc gaaatgaacg
    16561 ttttcgtgag accaacaact ctgctccggc ggaattgtgg aatcttctca aatagatgct
    16621 gagcgaatac caggcgggag gggcaatccc aagccgcccg ttcagcggat gaaaggcccg
    16681 gtcagatttc gacatggaaa caatctggat cgccgagaac aagctgggga ccaagctccg
    16741 ccggaatcgt gccttttggc ggggcgagct ggaggaatac ccgctgctct ggtttcccgt
    16801 tgagggcgcc cgcccgatgc cggcgcttcc ggagatctgg cccgaggaac gaatgtggac
    16861 cgatatcgaa tatgtgatgg aatcgacgga aaacagcctc gccggcacgt attacggcgg
    16921 cgattcgctg ccgatctact gcccgtggtt cggcccggac caggtcgccg cgtggctggg
    16981 ggcggagatc atcctgaaac cccgcgagaa cacgtcgtgg gtcaagccgt ttgtgaagga
    17041 ttgggaggag catccggagc ttcggatcga tcccgaaaac gaattgtgga agcgctacct
    17101 tgagatttac cgggccgccg tcgagcgggg caagggcaat tgggtaacgg gttatccgga
    17161 tcttcacacg ggcatcgatg cgctgagcgc cattcgcggc ccggatcatc tttcgctgga
    17221 tctgctgacg aacccggggc cgatcgagcg cgccatgatt cagatgaccc ggctgtggaa
    17281 ggaaattgtc gataccatca cggatatcat tctgcccggc gggcagggga cgaccaactg
    17341 gacctgggga tggagcgacg agcgttacct gtgcgtcggc cagaatgact acacctgcat
    17401 gatcagccgc gagatgtatg accgcttctg cctggcggac aatatcgaat gctgccgcca
    17461 tgtcgacatt tcgctctatc atctcgacgg cgtcggcgcg ttgcatcatc tggacaccat
    17521 cctcggcatt gaagagctgg attgcgtgca gtggatctgg ggcaacggga agccgcccgg
    17581 ttcgcattgg attgatctgc tgcggcaaat gcaggcggcc ggcaagctgg ttcaggtgct
    17641 gtacaatgcc gacctgctgc gcagtgggat cgacatacgc catgaaatcg aggttctctg
    17701 ccgcgcgctt gatccctcgc ggctgtgcat cgtcgcctat ctcgatgatg cccgcctcgc
    17761 ggatcatctg gtggaactgg cgcgggaaat ctgtgccgcg aagcgtccca cgcaaatcat
    17821 gatgtgatgg gaagaaaagg agcggcgccg cgcgacgcgt cagcccaacc gggatcgagg
    17881 gcaccatgaa acgccgccag ttcaatgcct acctcgcggc ggggcttgtt ctgtctcatc
    17941 tgccgtccgc gacgcgccgg ctgatcgcgg cgtctgatcc agctgccatg aaaggcggga
    18001 ccggcgccgg cggcccattg ttcgagctgg gcggcgcgcg ccatccgcac ggccagttta
    18061 cgcatgtcgg cctggacgaa gcggggcggc tctgtctcgt gaaaacagat tcccgctatg
    18121 ccgcggacgg tcgctatacc agccccgtgt ttgaagtccc cgagtcccaa cccgttgtca
    18181 ttcatgcgcg ccgacgctgg accggcccgt tgaggtggga acggcatcag ggcaaccccg
    18241 tgctgtcgcc ttcgcccgag ggttgggatc aggcccaggt gacggcgtgc agcgtcgtgc
    18301 gcgtcgcgga tcgctatcgg ctgtactatg gctcgcgggg ggccggcatt ggttttgccg
    18361 aggcgcccgt ggatgaaccg cacgcctggc gcaagcagga gcggccgtgc ctgaccgccg
    18421 ggccgccgga ggcgtttgac ggcaagggcg tcctgtcgcc cgaaatcgtc atcctggatg
    18481 acgggcgatg gtggatgtat tacgtcggct atcatcctga atgggagcag aacggcacgg
    18541 cggtgcatca gctcggcctc gcggaaagca cggacggcgg aatcacctgg aaccgcatca
    18601 gcccgcagcc gctgctggcc cgcggacccg ccggcagtta cgatggattt gccgtcagtt
    18661 caaactcggt gtggaccgat ggccgcgact ggctgatgtg gtacacgggg atcgcgcagg
    18721 tcccgtatct tgggagtatt tgcctcgcac gatcggaaga cgggcgatcc tggacgcggc
    18781 atgaggtcaa tcccgtgctg ccgtttaatc cgcacatcgc acacgaggcc ttttttgtcg
    18841 ccaagccgca tgttgtcaag gacggcaacg tcttccgcat gtggtacagc gccgtgggct
    18901 acggcgacga ctgcacgcag cccggtcagt accgcgtctg ctacgccgaa tccgctgatg
    18961 gcgtgcaatg ggaacgctgc gaggtcaatc ccgtgctgcg tcctgccgag gccggctggg
    19021 attcgcagat gacggaatac gccgaagtcc tccatgtcga acacggttgg cacatgtggt
    19081 attgcggcga cggctacggt cacatcggct atgcacgcgg atatcccgtc gccgcggcgg
    19141 agatccgcgc ccgcaccgga tccattcggg aaccggatga ttcgtggacc ccatggatcg
    19201 aggtggatgc cgctccgggc gcggccggat ttcaaccaac gggcgcgttc atgcagattg
    19261 aggtccggct gcgaaccgac gacactgcga tttcgccgcg cgtcgatttt ttgcgggtga
    19321 tgcgggacgc ctgacagccc ggttcactgg tcgaggagca tgcccatgat caccagccgt
    19381 caacgcctcc tggcaacgat tgccggcgag ccggtcgacc gcattcccgt cgccccgttt
    19441 attcactcca attatgttca tgccgtctgg agttccgccg aggtggacct cgtcgagaaa
    19501 accgccgaat tatattgtga ttttggtttc gacatgattc atcgcaattg cacgccggcc
    19561 tatgatgatc tgctgctgga gggggaaaac tggacatgcc aggttgaact ggagcagctc
    19621 ggcggcaacg acacgaatcg tcattacatc attcgcacgc ccgaaggcga gctccgccag
    19681 acgatcggca cgcgctggat ctcgaagtgg gagtgcgaag tcgcgccggt tgattatctc
    19741 atcaagagcg aagccgatct ggccttgctc gaaaagtacc agccgccggt gggcgagatc
    19801 gatggggagc ttatccggcg cgccgcgcgc gtggtcggcg aggacggggt cgtcgcgccg
    19861 tggatcaacg gtgtgttcaa tacggtggcc tattcctatc gcccgctggg cgaggtctcg
    19921 ctggatccgc tgctccagcc ggaattctac gatcggatga tgcgtcattt tctcggccgg
    19981 tgcgtcgcca tcgcgcgtca gttcattgcg gcgggcgccg attttctctc catcggcgtc
    20041 aatatggcat cgggcaagct gttcgacggc tcgttcattg aggcctacgt cctgccgtat
    20101 gagatggaat tctttgccgc gctgcgcgag gccggcgggg cgacgatcgc gcacaattgc
    20161 gggcacgcca gccggctgct gccgctttac gcgcggctcg gcacgacgat ttacgaatcg
    20221 ctgacgccgc cgccgcacgg cgacacgcgt atcgaggagg ccgttcgggt tctcggcccc
    20281 gaggtgacgc tgatgggcgg cctggaccag atcgacctgc tgcgccatgg atccccggcg
    20341 caggtcgagc aggccgtcag ccagatcatg caggccgttc attcccgctg ccgctacatt
    20401 ctcggcacga gcgactacct gctcgacgag acgccgccgg agaatttacg cgcgctggcc
    20461 cgcgccggcc acgagatggg cgtgttctga gtttcccgca gactcgtttg acccggcccc
    20521 agttgtccgg ccaccccttc cagggggttg gacgtctgga tcggcgacgc agggttccgg
    20581 gctgcggccg acaccctgcg ctttattccg gtcgtccgct tcgcgggctg agccaatcaa
    20641 caaattgaaa gcgcctgcgt tggaccgaga agtctggcag ctgtgtttaa tgcatggacg
    20701 gattgcgtct tgggggttgg cgaggttttc ggtggcggga aaggggcttt cgagagccga
    20761 gagctgagtt atcaaaagaa cggccgttca cggcctctct aaattcatca gatgaccgcc
    20821 gcgccggcgg tctcaggccg gcgtgggctg cttgatcaga attccctcca ggatgagctg
    20881 cgtcacgtgg ttgacccgct gctcgagcaa tcgcggcgcg tggatatcct tgcccagcag
    20941 catcgacagc gtgtactggt tggagaagaa gaagaagctc agcgccatgc aactgatcag
    21001 gagctggttc tcatccacgt cgctgcggaa ccggccgtcg cgcgcgccca tctgcagcga
    21061 gacgccgagc gccttgatca gcggggcctt gacggcagcg acgtcgatcg ccttggccgt
    21121 ccgcccgtgg cgcaggttct cccacgacag gatgcggacg aactcgggat tctcattcag
    21181 gaaccggtaa taaccctgga tgatcttctt gagcagttcc tcggccggga ggagcatgtg
    21241 ggccaggtcg acgtcgaccg agttcatccg gtcgtacatc gccttgagga cttcctcgta
    21301 gagcccttcc ttgctgccga aataatgata gagcatgcgc cggttcaggc cggaagcctc
    21361 cgagatcatc gagacggacg tggcgtcggg gccgtgttcg gcgaacagct tgatgccggc
    21421 ggccagaatc cggtcgcggt tcgaaatgcg cgggctgcgc tgtgttttcg cggctcttgg
    21481 cgcggacgtt ttcgtcgcca tcgctccctc cctgctcgat gccgacgggg ccaacgcttc
    21541 ccgccggaat cactctggtg gccgctatcg tagccatttg cgccggacct tgccaccttg
    21601 aaaccacgca atcacggccg gcgcgaccgc actggctcgc cgctcgcccg gcggcgcgct
    21661 ttcggttgac gcgccccggc ggccgccgta tgataaccgc tttgtgaacc atgtgaacca
    21721 ttcatttccc cctcccgggg agcccaaaag gatggaccgc caccgtgaca ccttccgaca
    21781 cgcgcgaagc cggcacgttc tgtctgcgct ttgacatcga tacgaatttc acgccgtggc
    21841 ggcgcaacaa tttctgttcc cgcttcggtg aggacgaacc gcggttcatt ctccggctgc
    21901 tggactggct gcgggagcgc aactgccgcg cccacttctt tgcgatgggc ttcggcgtca
    21961 tcgactacgg cgaatcgctg cgccagatcg cccagtccga atacccgctc gacagccacc
    22021 ttcacacgca ccgcgtcacg ctgctcgacg actacaacgc cgtctacgag gagatgatga
    22081 cggcgcatca tctgctcgcg agcatcggcg cgcggatcga ggggatcggc gcgacgggga
    22141 tgtatcccga ggggatcgac gggcgacccg acatccagga gctgatcctg caaaagggct
    22201 accggtgggt ctccagccgc tggaacctga cgcgcacgct cgaacagatg cagccctaca
    22261 ggctgcccac cggcctgctc gagattccct gcgccgggtg gtccgacatg acgttctttc
    22321 acatcaatga gcagcgagcg ctggcgccgg agtatcaggt cgagcgctac gaactatcca
    22381 cctttaccca gcatgtgaag gacatgatca cgcgcgcggg cagggagaaa ctcgactatg
    22441 cgatctgcct gcaccccgcc gtactcgcgc tgcacgatcc ggaaatgacc tttctggagc
    22501 aggtgacggc gcacgcccgc gccgaggggg tggaactcgt cgacttgcgc caggttcatc
    22561 agcgccatgc ggccgtggcc gcggccgcgc gatgagcgcg gcgaaaggga ctcaagggac
    22621 gcaagggaca taggaagaat cagaagtttg ccggccgaaa agattgcgcc gaggttttcg
    22681 gccgtcgtct tcccgaatag ccgttgactc tacataaacg atcagttacc gttctcttga
    22741 atcgccattc gacccacatc cggaatcgac cgggcccgcc acgccgttgc ggcggcgccc
    22801 cactcccgac gcgattcagc cgccatggca acgttccgac cacgcgtttc cgcccggatt
    22861 cctctgccgc aggggtggcg attcgtggcc ccgcgtcccg atctggcgcc ggcgcacgga
    22921 attgacgccg gtcccggcgg ccggcgcgcg gcggccttgc gcatctcggc caccggcgat
    22981 ccattggctt ttggctggtg ggtccggcga ttgacgctca aacccggcgg atggtaccgc
    23041 gcccgcgtcg ccttccgaac taccgacgac gtggacccca gcctttgcgt cctggtcaat
    23101 ctgttctggg gcgagtatcc gacgcggctc aagcgcgagg aattcttcag tcatttttcg
    23161 gatgcgggcg acggctgggt gtttgccgaa cacctgttcc agatgccgga tggcgagaag
    23221 caggttgacc tgcagttgca tctgcattcg gcggccgccg gttccgtctg gttcgccaat
    23281 ccggccatcg agccggcgca agccccgccc gaccgtcccg tcacgctggc cacgacgtgg
    23341 ttccgccgcg tggacggatc gagccgcgcc ggcatgctca agcggattgc gtcgcggctg
    23401 gatgaggccg gtcatgccga tgccgatgtc gtcctgatcc ccgaatgcgt cacgggacac
    23461 ggcgtcgcgg gcaaggatca tcgcgctcag atcgagcagg atgccgagcc gctcggcggt
    23521 cccgccaccc gcctgtatca ggaccgcgcc cggcgccatc gcatgtatgt gcttggctgg
    23581 attcatgagc gcgacggcaa gcgtattttt aacacgaccc ttgtgattga tcggaaaggg
    23641 cggatcgtcg gcacttatcg caaatgccac ctgcactggc cggagctctg gtgcgggatt
    23701 tcaccgggcc atgcgctgcc ggtatttgac accgatttcg cgcgcatcgg catctacacg
    23761 tgctatgaaa actggctgcc gctcgtcgcg cagaatctgg cgatgaaggg ggccgaaatc
    23821 ctgttcctgc ccaccgccgg gtatctggtc gacgtgttcc gcacgcgctg cctcgacaac
    23881 aattgttacg gcgttgtcgc cggcctgaat tcgccaagct gcattgtcgc cagcgacggc
    23941 acgctgatgg ccgagctccg caacgacggg ctgatcacgg cgcgggcgaa cctggccgtg
    24001 cggccgacgc atgcgtatgc ctaccgtcac cctaatctga cctatgggct gccgggcgcc
    24061 gatcggctgc tcgatcacag cctgagccgc atggctctgg atgaaatctc ggcgctgacg
    24121 cacggtccca tcgtgggcgg ccccgcgccg cgcccctacg tgcgggatcc gggggatgag
    24181 cgctagcccg cgggctgacc gggcggatcg ggcgcgcgcg gttgcctggt attagctttt
    24241 tcgcggttgg cttaattcca ttggaatatg gtcgtgatcc acgaaataga catatcggtt
    24301 cccggcattc ggtcccccct cgacggtcat cgcctcggac aggaacctcg ccccatggcg
    24361 ctccagccgc tccatttccg catcgatatc gtccacaacg aagcagaggt gggcgctccc
    24421 gacgtcgcac gggcggggag caaccgggca gcccgccggc gcaaaatact ggatcagctc
    24481 cagcccgatc ccgtggccgc gcagatgaac cagccgcaga tgcgcccctt cgagttgaat
    24541 caccttctcg gggaccggcc cctggatcgt ccggtcgctg aacggctcaa atcccaaccc
    24601 gtcgcgataa aaggcaatgg atcggtccat atccctgaca caaaaggaga cgtgatgaat
    24661 acccgtaatc atgaggctcc cctcgcccgt tccggcgaat cgaacatcgc tgttcggcgg
    24721 ccgcggcttg ccgcgcttcc gaccatccct aacagaattc gggtgtggaa acaagccgtc
    24781 tcggccgtcc gctgcgttgc ctgcattgcg gcgctcgcgc tggcggcatc ctccgtctcc
    24841 gccgcggact ggccgacgtt caaacacgac cggcggctga cgggcaggac gccggccaag
    24901 ggcgcggtca cggccgagcc ggaaattctc ggcgagtatg cgctgacggg cgtcgtcacg
    24961 acgctgatgg ccgacctcaa tgccacgtcg acctcctttc aggatgtcgc gctgccggac
    25021 gaacctgcgc tggccggcgc tcagtggacc gaacccgcgc cgctcatcga tctgcgcggc
    25081 gacggcgtgc tcgtcccgcc gcccggcgga cccgaaacgc tcaccggcgc cgtcgatcag
    25141 ctcgttgccc aggtcctgaa ggatcagccg ggctaccagt cgatcaagtg gcatgactat
    25201 tactccgagg aaccgaccgg ctacatggag atgctgtcgt atgaagcggg cgcggaggaa
    25261 ccgcggcagg tctggcgttc gacgccgctc gagccgggcg tttatgaaac gctgatgctg
    25321 gcggtcgacg ccaacaacga cggccagaag gatatcgcgc tggcgacgca ttaccacgtg
    25381 gcgatctggg acggggcgac cggcaagaaa atcatggagg tgcggccccc cgtcgcgcgc
    25441 aattacggct ctttcggcta tttctttgtc gaagggaacc cgtatccgca atttttcacg
    25501 atcgtcgact ttcagagcca tttcgacgtg tatggcaatg acggcaagga gctgacggtc
    25561 ctctggcggg aggacgtgga aaatcccaat attctgcgtg tgcggaaaat tgtccgcgtc
    25621 ggacccgatc ccgtggccga catcaatggc gacggccggc tcgaactggt ctttaattat
    25681 tacgaccgca ccggcgaccg ggaatggcac gtgatgggct acgacgcgct gacgggcgag
    25741 atcgtcctcg acctgccgga gacctttctc gacggcatcg ccgacatcac cggggacggg
    25801 ttccccgaaa tgttctgcaa atcaacgaaa gggcagaaga cgaaagcgcc cggcgagctt
    25861 cgcatctaca gcgcagccgg cgggggcgat cccgcaccga tctggcgccg gccgaactcg
    25921 ctctgggtca cgcacgatgc cccgcgccgg gccgatctcg tctccatcgg cacggacaat
    25981 cgccggaccg tcatgctcgc caacggcccc aagcccggtc ttaaatcgtt cttcgtgacc
    26041 gaatcggcgg gggaacgcca gacgctgcgc ggcttccgcc tcgatgaggc gcgcgaggcg
    26101 ctccccggac tccagctcga gctggacggg gtgaaatccc tgcagatctc cgcgattgcc
    26161 gatgtcgatc aggacggcat cgacgagctg aaactggagt gcgtcggccg ggatcccgtg
    26221 gccacggcga aagccaatgg ccgagtggtc cggctcgcga tgcaggaaac cacgggcctg
    26281 accgaccccc cgattgtcat ttcccgcaac gaaggcccgc cgctggtcgc catccccacg
    26341 atgacccgcg agtatgtcgg cctgacgttc tccgagggcc aatttcatga acaatggcgg
    26401 cttcccggct ggggcatgcc gggcgcgacc ttcacgcaaa tcgggctgac ctcggccgac
    26461 ctcgacggcg atggcgcgcg cgagatcctg gccgccggca agaacgaaag gggcgaggcg
    26521 accgtccagg cgtcggatct ggccggcaat ccgctgtgga cccaggcctt cgcggaattt
    26581 gacggcgacc cgccgatctg gaacgtcggc gggctgaccc acattttcgc cggccgtttc
    26641 agcgatccgc aacgcgacga cgtgctcgtc tccgtccgcc gcagcatcat gcacagcgac
    26701 gaaggccaca tgctcgacgg cgcgaccggc gaggaacgct ggagcgccga tcgcgtcggc
    26761 atcgaggccc ccggccaggg cgacacgggc tacgggtttc tcggcaacaa tattgccgcc
    26821 gtcgatctga atgccgatga acgcgatgaa atcgtcggcg tctatcccgt cgatctctac
    26881 gccttctcgg gcgacggcga gcagttgatg ggccgctttc tcgatggctg gggaatctac
    26941 ggccagcatt tcgtccacga tttcctgggt tccggcacgc cgcagatcct ttcggccgcg
    27001 cccggttcgg tcgcgctgct ctccagcgag ggggagatca tctggaagga tggcgagggc
    27061 gagaaggccg gcacgacgtg gccgctcgcg ctgaccgaca tggacggcga cggcgcgatg
    27121 gaggccagcg gcgccggatt cgaggacgga ttccgcgtct atgacggcgc caccggcgag
    27181 gtgctgcacc gcatggcggt cgagaagtcg accaaccgga tcgtcccggc ggcgggcgat
    27241 ctggatggcg acggccgcga cgaggtcctc tgggcggtca accagacgct ctacgccgcc
    27301 ggcctgaagg acggcgcgtt ggggattgaa tggaagaagg agttccccgc cctggtgcgc
    27361 ttcccggtcc tggccgacat caccggcgat gggctggtcg aaatcctcgt ctcgtgccat
    27421 aatggccggc tctatgtcct ggggaactga ccgcctggca cctccgcccg aatgaagtca
    27481 ccgcgttgaa cacttggaat gacgtcgtcc agccttttct cagttcgcag gccctggtct
    27541 gggggccgat ggtcgccgcc catcaactgc ggtcgcgcgg ctggatccgc gaaagcatcg
    27601 gcaagccgat gcataccgtc acgttcacgc tgttcgggcc gctgatctac gctcttggcg
    27661 tctggcggct cgatctctcc ttcgatcgct ggtatctcgt cccgatcgcc aatgcgatcc
    27721 tgctgattct ctccacgctg gcggccgtga ttctggtccc cgtgctcgcc cgttcgcgcc
    27781 aggcgcgcgg gacgctcagc atcatgatcc cgatcagcaa ccagggctac acgctggcgg
    27841 ggtttctgac gattctgttc atttcggacg aagcgtacgg ctataacgcg ctgctgatga
    27901 tcccggccgg tttcctgctg ccgctgctct ggctgccgct ggccgagcac tggggccgcg
    27961 gcgagcacac ctcgatctgg gtcaactatc gccgggggat gttctcgccg atgtcgctgc
    28021 ccctggtggg ggtgatcatc gggcttcttc tgaacatgct ggccgtgccg atgccggcgg
    28081 tcgccgcggg gggcttgcgc tggctcgtgt tcctcgccac ggccaacacg atgttcgctc
    28141 tgggcgcctg tctgcacgtg cgccatctgt tcgggttttt ccgcacgctg cgctgggtct
    28201 ttctgttcaa attcgtgctc catcccgcgc tgatgttcgg gctctgcctg ctgttgcagc
    28261 tcaaggggat ggccgccgcc gcgctgttca ttgccagcat catgcccgcc ggcgtgatgt
    28321 cggtcagtct gtcgagtatc tatcggctcg atctgaacct gggcaatgcg ggttatctct
    28381 ggacgacgct ggcgttcatg atcattgtgc tgccgattct gatctttctg ctgcggttgc
    28441 cgctgttcgc gtattgaggc ccggcgccgc cgcgccgcgg ttgatcgcct cgggatggcg
    28501 gcggtaatag tcgtcgatca gctcggcggc gccggccagg agcatgcgcg cggcctccag
    28561 cggttccagc tcgttgaaca gcacgcgctc caggatctgg cgtccctcgc cggcgaaaag
    28621 gtaactgccg ccggaactgc ccgagatgat tcgcagcagg tcccggcgcg tcttcaagtc
    28681 cggccagtcc ccgcccatgt tggaataagt ccgcgccgac ttcatccatc cctcatacag
    28741 ctcccagatg taagtcagcc aggggtcgtt gtcgatcacg ctcggaacgt cgcgggtcag
    28801 cgcttcgacc gcttccgcat cgggccacag atgcagccgc atccccttgc tctcgacgta
    28861 atagatcgcc tgcacctcgg gcgaggccag atagcgcgcc acctcgaggg cggcgctcgt
    28921 gtgcgcatca ccgcggaacg ggatctgctt catcacgccc cagccatcgg ccccgacgcc
    28981 gaacacgtaa tcttcgccgt ggggcgtcgg caccggcacg ttggtagtcc gcagcgggcg
    29041 ttcgcgcagg cccttgtcgt attcctgctg cttgagcatc tggtagctga cgctgtagcc
    29101 cggcgtgttg gtaaaggtgg cgctcagttc ctgccgctcg cacaattcgg cgacgatctg
    29161 gccgaagtcg agcccgagga atttccgccc ccacagcttg tcgcggtgga tcaggtcctg
    29221 catgaaccgc agggggatgg ccagcgtctc cggcgtgatc gccgggtcga gccggtatcg
    29281 gcccgccgcc tcgtcgtaga gcagaaactg gcgctcgacg acctcggggc ccacgatcgg
    29341 cggcagcagg cattcgtaga gcagtgtcgg gacgttgagc agattcagtc cgaaggcgta
    29401 ggcctcgggc ccgtatttgg cgcgcacggc ggtctgcacc ttctgcattt cgcgcttgaa
    29461 cgtctcgtaa tcccagccgt gctcgaggta atagcgcaga tccacgcccg ccagctcgaa
    29521 gtgctcgcga ttgccgacga agacgccgcc cgtcaggtag gtccgcgagg ggaactgcgt
    29581 gatcagtccc tcggcggtga tcgcgcgcat gatggacggg ccccaggccg cgcgcatggc
    29641 gggggaaacc atcgattccg gaatcgggac aatcagttcg atcagttgct cgcgcttggg
    29701 caggttcccc acgacgttgt aggcattgtt gacaacgtcg ggcgggctcc cgctcgagat
    29761 cgccagattc agcttggcca ggtagaccgt gtaggggatc agcgtcaggt caatctggat
    29821 gtcgtcgcgt tcgcggttga agcgctcgac cgccttgatc gtggcttgct cgtcccagcg
    29881 tcccatgatg acgccaatcc agacgctgat gacctcgggg cctgcggccg ggtcgccgcc
    29941 ggccgactgg ccgcgcgggg tggtccggcg agcttccgcg gcgagcaagc cggcactcgc
    30001 cagcagcagg atccattgca gaacgactcg gatgcgaagg ggaagcatgg atgaatcgcc
    30061 ggcctgttgc atgcatcggc tgagtttttc agcccttgat tccaccaaag gtaatgcctt
    30121 tgacgaagtg ccgctgggcg gcaaagaaga cgcaaatcac ggggattgac gccacgagcg
    30181 acgcggccat gatttcgcca tagtccagtt ccccggcata gccgccgtcg acgccgagga
    30241 ccccgaggat gcccatctgc acggtgtaca tcgcgcgcga gtcgatcacc aggtaggtcc
    30301 agaaatactc cagccacgcg ccgacgaacc ccatgatggc cagcacgccc agcaacggcc
    30361 ggctgagcgg caggatgatg tgccagaaca ggccccactc gccgcagccg tccatcgtcg
    30421 ccgattcgag caactccccg ggcagcgtct gcacgaactg ccgggagagg aagaccgtcg
    30481 cgatgccggc catgaacggg acgatcagcg ccgtgtaggt gtcgttccag ttgaactgcc
    30541 acaccatctc gtagagcggg atcaggatga cgaactgcgg gatggtcatc gtggcgatca
    30601 tcagccagaa gaggaagcgc gacccgggaa accgcatttt gccgagcgca tagcccgcca
    30661 tcacgccgaa caggatgttc gtcgcaaccg gcagggtgga aacgatcgcc gaattgatca
    30721 gccagcggcc gaggtagccg ctgacccgat ggttgaacgc gaacaggttg ttcaggctcg
    30781 gattgcgcgg gtacaactgc aaatcgttga cgtcgaacgt cacgcccagc ggaatcaggc
    30841 tcgagacgat catcaggtac agcggcagca gcgccaggaa cgtgtagaac agcagcaccg
    30901 agaaggcgag gccctgcccc agcaggctgc ggagatcgcg cttaatactc gacatcggtg
    30961 gccaggaacc tgtagttgat gacggcgatc agcacaatga tggccatcag gaccacgctc
    31021 atcgcgcagg ccagcccaaa ctgattctcg tgatagaagg cctggtagat gcggtaattc
    31081 acgaaatagg tggtatactg cgggccgccg ccggtgatca gcagcgcggg ggcgaaaatc
    31141 tggaacccgc cgatcatccc cgtgatcagg acatagaccg tcgagggctt cgcgagcggc
    31201 cacgtgatgc gcaggaactt gtgatacgcc gtggcgccgt cgatatccgc cgcctcgtaa
    31261 tacgagcggg ggatattgcc cagcgccgcg cagtaaatga tcacgccgat gccgttggtc
    31321 gagagccagc cgatgacgat cagcgtcggc agcgccaggt tcggatcggc gaaccatgtc
    31381 aggttttctg agatcaggtg gagccgcgtg aggagcatgt tggcgaagcc gtcgccggga
    31441 tagaaaatcc acttcgtgat cgccgccgaa accaccacgc tgatcacgcc cggcagatac
    31501 agcgcggcct tgaagaaggt ctgccagaat gcagggagcg aaacgatcag cgaggccaga
    31561 aagaggctca tcaggacgct gtaggggatc gtgccggcga tatagatcga ggtgaccgcg
    31621 acgcttttcc accacagcgg gtcggcgatg atccgcgcgt agttgtccag tccgacccac
    31681 tgcgtgccgg actgcacgtc catcgatttg tcggtgaaac tgtagatcag cgatttggcc
    31741 agcggataga acagaaacag cgtgctccag ccgaaggcca ccgcgagaaa cgcataggcc
    31801 gcgtgccagt gcgagaacag tcgcgtcggc gcgtgcgccg agacggccgc cggcccgccg
    31861 gcccgcttgc gccggatgag gtacgacgcc atgcacagcg aaggcggcac cgtcgcccag
    31921 ccgattacga gcatcgccag tgctaaatac ggaactggat tcatcgcctc acaatacgac
    31981 acaacggcgc gtgacgtgaa gccccgaatg gcccggagct tgaagcggcc gcgcttccgc
    32041 cgacccggcg tcccgcctgg cgtggcctgg gccgctcggc cgcccgcgtt tcatcacgcg
    32101 ccgtctgtcc tgtcgggatc g
//
LOCUS       DAOPCO010000014        32030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1556_length_32030_cov_5.545708, whole genome shotgun sequence.
ACCESSION   DAOPCO010000014 DAOPCO010000000
VERSION     DAOPCO010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32030
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1556_length_32030_cov_5.545708"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..394)
                     /gene="ssrA"
                     /locus_tag="PLS90_02195"
     tmRNA           complement(32..394)
                     /gene="ssrA"
                     /locus_tag="PLS90_02195"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            928..2316
                     /locus_tag="PLS90_02200"
     CDS             928..2316
                     /locus_tag="PLS90_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK01245.1"
                     /translation="MPSPRLLIAEDEERLRRLLGMLMANAGYKLTMVADGQEAREQLD
                     AHDFDLIITDIKMPRMDGMQLLAYVREHKPDIPVVVITAYGSIESAVEAMQAGASDYL
                     TKPFEEEKLKLAVERGLRIRKILAENRQLRREVDSRWGLDQIIAHSPAMREAIAMARQ
                     VAQSNANVIVYGESGTGKELITRGIHNAGPRASGPFVAINCAAIPDNLLESELFGHEK
                     GSFTGAIERKPGKFELAAGGTLFLDEIGDLSLHVQAKVLRAIENREIQRVGGTEVLEV
                     DVRFIAATNKDLQAEAAAGRFREDLFFRLNVFPIYLAPLRNRREDILPLVNLFLNRFC
                     AQMGKRVPRIPSETRDRLLADPWEGNVRELQNTIERAVILLQGDVLAPELIRKISGPS
                     LRGAETASTRFELPPEGFSLEEHEQELIRQALQRSRFKKAAAARLLGLSRATLRYRME
                     KYGIPDKPAPPG"
     gene            complement(2345..3418)
                     /locus_tag="PLS90_02205"
     CDS             complement(2345..3418)
                     /locus_tag="PLS90_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK01246.1"
                     /translation="MKSFRSAILLALLVGLCIGGAAPARAGSGTPEDPWIIGMSQCNL
                     AEPWRAQMNKDIADAAAAHPEVRAIFKDAQNDTLVQRQHIEEFVNQGVDVLLVSPKEA
                     VPLTEPVARAYQAGIPVMVLDRRIMGDQYTTFIGADNKLIGQAVGRWIARQTGGTGNI
                     VELKGLMTSTPGQDRHSGFLKGIEGTDLKVIFEADMEWLEPKARQEMESALARFPDID
                     VVYGHNDPAAHGAYLAARAAGRADEMIFVGIDALPHEGKRYVEEGILDASFEYPTGGR
                     EAIATAVEILLGKEAPKEIVLPSRFFTADNVKDNGLWLEEAGLIDMPGEEPSNLEPNQ
                     PVIEERLRIGEEVGEDIKELPTD"
     gene            3896..5878
                     /locus_tag="PLS90_02210"
     CDS             3896..5878
                     /locus_tag="PLS90_02210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPK01247.1"
                     /translation="MARLDWLQRLRPRRFPRRSHARFNRIARRWLFAGLSGLLALYLF
                     TLLFDMEPFLIRTELGLFQWLDGHLGKDSVLDWTVLVGASRFWVYTSLAVVLFAALAQ
                     GWAMRRRDYGQVFGLIFFAAVVTILAEEVGDFVSDHINRDLPWDSGALETIRDRYDAD
                     FLDIPSTGGIVDENMVGWGVMILLLSLRLPRPAWIALALGLVHAGARLTVGAQWLFDE
                     VNSALLSLTLAGGALLGLGRALRWCERNASEQIILLTGFHLAPPEAGHAAVRAELTHN
                     VAEARRLHRLRRHRFWENLTRRSVLPVLNATGRPATLAAQPPGRTIPGFRPSPHVRFL
                     ALEGGELFVIRAMRRWGGWLAPSAKFRRYVQSARCHLYLERLGLPVPRMFWVRETLTT
                     LGLRNLYFLVEEFIDGRPVDRDSQAELRAAVGLLVRLHGFESETWGPVAQAAPQPRSH
                     YLLRALRPDLLYYLRRIEKWADVRFDHDDLERLWKLEEAAALAVLLRRRRPFRLIHGD
                     VHYRNFLYGPARGEVHLIDFTTVAYDLHGWEILKMAAAFGRREAPAAARLWREYFELA
                     SEERWGEFLHEAPLALFRHGLREMAHQRVAPPRAPAALRKASILEWLAGLLAPAPELW
                     GARAAETDWEALLERMRPDLCVAAGVQPPADSSVLP"
     gene            6161..7828
                     /locus_tag="PLS90_02215"
     CDS             6161..7828
                     /locus_tag="PLS90_02215"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HPK01248.1"
                     /translation="MHPENRPPVRTIAREARGNDPRIVLFIALFLALFTMLVVVGQAQ
                     QAEPPSDAAIQRTTTIEQTTITRRDGAPPLELSDLGMKLAIERELARAPGINTATLDI
                     DVSDGIATITGSAPNVIVRDRVERIAATVRGVTAVVNHLTIESADVADPVILANLQRA
                     LGADPALDPGLLQISIDSGIVTLSGSVDSWRERWLAEEAAKNVYGVRGVVNQIQLEYP
                     EGTAGRADDEIQQQIIDRINGSALIDAGLVAVGVRDGQVSLSGIVPSLADKVTAMRLA
                     WVSGVTSVTADELKVDWELYRTQYTPPAAPSDAPVRSDSVREQARDALMLNPYVEQEN
                     VEVVINNGVATLSGTVRSLTAKREAAEAVRHVGGIFEVRNNLMIDPGIEATDAAIAQS
                     VSDALRVDPVVEHVEVDVAVDQGIVTLRGQVDDAFERARAEEVAGRIAGAVAVANELT
                     IAQPEAVATPSAARSDAQIAADIESELFWSALVDPSDVQVQVQDGVATLRGRVDDSRE
                     YGAAIRNAFEGGARSVVSELTTTNGPVGPATHGEPIVYDSPLMSEFR"
     gene            complement(7893..8663)
                     /locus_tag="PLS90_02220"
     CDS             complement(7893..8663)
                     /locus_tag="PLS90_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01249.1"
                     /translation="MAAPGAQSLWSGLREVVAHLTRAAYWTERRDACDLLHLTARQAL
                     LQLRRAARDSDPDVKHWGEQYLTQIRRDLDSDVEELAATLDAALSRDVVDETGATPVA
                     ATGPAAAPPRDARDDGPRDAEDLIRWLEQWTAEQGGPFERREAGAACVLPVGDARRQR
                     IFLDAARADSSGQPVAMFYSLCGEADPDTYQWALEANASLSRGAFAVIQHRERRMLIM
                     RLRRSLRDLRYATLPRKLLYLARKADWAESRLKEQDRH"
     gene            complement(8735..9742)
                     /locus_tag="PLS90_02225"
     CDS             complement(8735..9742)
                     /locus_tag="PLS90_02225"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPK01250.1"
                     /translation="MARIHPTYSGPLVFGGSAGKHLSREVCKRLGLHEGQLEVTRFSD
                     GETGVKICENVRGADCYLIQPTCPPTNENLIELLLTVDAMRRASAARINVIMPYFGYA
                     RQDRKDQGRVALSAKLVANLIATAGVDRVICLDLHTAQIQGFFDIVVDHLYAGPVITK
                     FLQRQEWARDLVVVSPDVGNVKMCRSYAQRLNASLAIIDKRRPAANVCEVMNIIGEVK
                     GRHCLLVDDMIDTAGSVCQAAHALREAGALSVSACATHPVLSGPARQRLQEAPLERIV
                     VTNSIPQRNDQTLAKLKVVNIAPLIAEAIHRIHNYLSVSALFDPNVDDEPEMADEDMT
                     G"
     gene            10049..10876
                     /locus_tag="PLS90_02230"
     CDS             10049..10876
                     /locus_tag="PLS90_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK01251.1"
                     /translation="MMSEPQGERAQAYPPLKIRVLGSGTSMGVPVVGCRCAVCTSSDP
                     RNKRMRSSISVQAGDTWILIDCSIDFRMQLLTWPMPRIDALLVTHTHSDHINGIDDLR
                     CYNYLQRGPIPLYSTEYFLNDLRVRFPYCFNPLQRGGGVPEVDLIGVEPGRPFDCGGV
                     RILPLAIMHGRLSILGFRFGPFAYMTDCSAIPEETLAKLAGVRVVILSALRHTPHPTH
                     FTVEQAVEAARLIGVEQAYFTHIADELDHEATNRKLPPWARLAYDGQLIELAATGGA"
     gene            10876..13563
                     /locus_tag="PLS90_02235"
     CDS             10876..13563
                     /locus_tag="PLS90_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK01252.1"
                     /translation="MGRRRRKPAAGQAGRRAAPAAAAAAPGVEPRPSRRDLPWRNAAR
                     DPWLWGLLALAALLRLPSLGVPSLWFDEIRTLYMARDSILNVQSHFGFWWVTLAFLQL
                     PLSTDIALRLFPALCGVAGVPVSYLIGYALRGHRVARWCAAIVALSVFHLFYSQEARF
                     YAPMYLLTALSWLFTIRLFARSAPAWFFAAAACDVAAYTMHPTQTVYLGLHLAYVIGA
                     LALTRPGHAITREYFMPVRARSEGGGINAASTIRLVVAAATVIGSAWYARRFLSRITQ
                     INFDPEYLIGGLTPRLVGRHVQAFGGQFQLWDLPGGLYAWLYLAMLLVGLCVAWRRWR
                     RDCVFAVMLTGGTFVASRGYEVSIDYAVKYVMFLMPFWAALAAQGFAWTARVGGDRLA
                     ARLPRLAARARLAPTCALVLIMAVASAFACFGYYSRGKIREKAAVRWLRDRLQPADEV
                     MVYGHSALPINFYWRKFGLPADQYHALWWEPNELGGNLFGSMEALRRPGGKVYFIDSW
                     DKDDPPRMAELLAQRGRMLREWPSFMGQPVRVYEIVPRAAPAGWPVQLDPLGPRITRN
                     PSRPYQGEVAGQEALILRDNSTVLYDFTLAEGGPCHLQLQANAGPVQVRIIEVLVDGR
                     LQGMVSFLAQEPAWLTRHVDLGELGPGEHVLTLRYLSRSGRLELEGATVEGVALRSLS
                     IHPGPPPVGDSDLVRLDFMDANYPSLALRADDGTTGDLNPDWQPLNLPDWELVRVPAV
                     DAPTTDGLTDAVLITMPPEFDGFARLLFDRAFPLTEQRVAYFAFDVRTERLYNHSINV
                     GFLFLDESNEPVLQHMAYSHGLFDDMNCTGYLGEEWTEGWVPMAAIDFAPAGAMWFSP
                     FIVLYDNPVRPSRHSGRVWIKDPRLQPIDTAPPVRAAAP"
     gene            13560..14768
                     /locus_tag="PLS90_02240"
     CDS             13560..14768
                     /locus_tag="PLS90_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01253.1"
                     /translation="MTAFLLTFGGFTFAMLAAFLVARGVTGWARALALRCGVISHVDH
                     RSSHTRPTPRLGGLGLAAGFALPALGFLLLLWFIPHRGAAMGYHPAPLLWVGLGWLLM
                     LAVGLVDDARNLPPLVKLALTALAALAPVAGGGVSFTFAQGFFFSTAAWSVALALLSA
                     GWILFFTHGFNFMDGMDGFAANFARVAALGIFGVAFVPCLLSGNMNLLRAEAYLLPIL
                     AMACWGFLHWNRPPARVFMGDGGSLSTGYLLATWTVMGHRGMLGFPVPLPLALAILMP
                     FLFDVLLTLARRLHRGENLLRAHREHLYQRLLRTGLGHGEVLRLNLPRFVGCAAPAVL
                     TASLAAVLNSGWVRGRFGEGPIARIHAPFVLPALHLLALGAALLIMVQYWCMVRRREA
                     RSGAEEAAGG"
     gene            14963..15310
                     /locus_tag="PLS90_02245"
     CDS             14963..15310
                     /locus_tag="PLS90_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01254.1"
                     /translation="MMDQVQQLEERVEAIMRLLDAARQANSRLEQENSELREQLARME
                     ELDSTNHQLRRQVETLENEMEGMASKETQIRDRLRTILERIDTLEHDVNAQIGQPDVF
                     ANSNGQPNEEQAG"
     gene            15334..15654
                     /locus_tag="PLS90_02250"
     CDS             15334..15654
                     /locus_tag="PLS90_02250"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPK01255.1"
                     /translation="MHPITITIADQSFRVLVEEGEEKRYERVQQLADEAYHEILRTGA
                     NAGLRAMAMALFQVAMDLDEARQLLRKAESSRDRLSDLIRRIDRATERSAHAAPPPAP
                     DRAE"
     gene            15780..16787
                     /gene="recA"
                     /locus_tag="PLS90_02255"
     CDS             15780..16787
                     /gene="recA"
                     /locus_tag="PLS90_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPK01256.1"
                     /translation="MLDVALSQIEKNYGKGSIMKFGDWHAENIPAISTGSISLDLALG
                     VGGIPRGRIVEIFGPESSGKTTLALHVVANAQKEGGIAAYIDAEHAIDPIYTRKLGVN
                     LDELVISQPDYGEQALDIAEQLVRSNSVDVIVVDSVAALVPKAEIEGEMGDSFVGLQA
                     RLMSQALRKLTGAVARSKTSLIFINQIREKIGVMFGSPETTTGGRALKFYSSIRLDIR
                     RIASLKDGDQNIGNRVRVKVVKNKCAPPFRQTEFDILFGEGISYEGDLLDLAVAHKVI
                     DKSGAWFSFQNERIGQGRENARQFLKTNPDVTKRVDAIVREHAMPSFGEESETPVSVE
                     D"
     gene            16844..17578
                     /gene="rph"
                     /locus_tag="PLS90_02260"
     CDS             16844..17578
                     /gene="rph"
                     /locus_tag="PLS90_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPK01257.1"
                     /translation="MLRVDGRQPDQMRAVNITPHYMEYAEGSCLIEVGKTRVICTATI
                     ENRVPPHVFGTGRGWVTAEYAMLPRSSQQRIVRDGIRGKVGGRSHEIQRLIGRAIRSV
                     FDLDRFGERTVLIDCDVITADGGTRCASITGALVAVGLAMERLRAEKKLSIQPLKAYL
                     AAVSVGVVEGQPVLDLCYLEDAQAEVDMNVVLTGRREFVEIQGTAETRPFDMATLLEL
                     NRLAGEGVGRLVELQHEILGLDLERS"
     gene            17646..18236
                     /locus_tag="PLS90_02265"
     CDS             17646..18236
                     /locus_tag="PLS90_02265"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HPK01258.1"
                     /translation="MFPVAVVPADRRRLGPLGAMTRACAWALAGCLLLAGCAREPLIR
                     DRRVMVMDFENAIDDPAQSHLSGGLAEMMTSQLVNYPRVAVIERQDVAAYFDKARTDP
                     QRWHELGRKSNIDYFIIGSIARLEDNYVLNARILSVATGQIVSGSSVTRSCAREEDIY
                     PTMQAVTRAIARNIQVLAERFDRLATGGEAAVATAQ"
     gene            18298..18690
                     /locus_tag="PLS90_02270"
     CDS             18298..18690
                     /locus_tag="PLS90_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPK01259.1"
                     /translation="MSRVGQPLAARRAGRLLALLALLALPAALTGCIRSRVFITSEPP
                     GAEVLWRGEPYGATPIEIPYLWYWHYDYALEKPGYERIDVVDRFRTPPWFLMPVDLFM
                     EMLPIPIHDTRERHFVLTPREPTLSQLP"
     gene            18736..19728
                     /locus_tag="PLS90_02275"
     CDS             18736..19728
                     /locus_tag="PLS90_02275"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK01260.1"
                     /translation="MRPRRTVILPPPARLATLVGLVLLAAATAGALPTDAIVARVNGT
                     PILLSELKEAALNQDIPIDALTLDGLRGEKYRLVLTQLVDEELLVQEARQSEIKVDDV
                     EIARLVDAMINQLRVELGGMEALEAFLRKHSLTLDSLRRMLTRRERRGALAARVVARR
                     VNLDAEALADFARRRREAGLPLEEVNLAQILLVCPPALRGTREGEEVHTRALRLAREI
                     GGDTSRFTELAMQHTEDPAGRSRGGYIGWMDPQGLLGPLRDAVAAMDPGEVSAPVATD
                     EGYHVLLLLGRRSTRDLMFAEQFEKQRAELLTQLRQNASIQLFDLDGNPIETIQ"
     gene            complement(19770..20432)
                     /locus_tag="PLS90_02280"
     CDS             complement(19770..20432)
                     /locus_tag="PLS90_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPK01261.1"
                     /translation="MVESQPLNVAVLVGMHPYDAIRFHDLFAAMDGIKPVHQMVENWA
                     FAEPEQKAEYDVVLFYNMHLKMEAELPWASPAVPKAIAELGDRPQGIVVLHHAILAYP
                     DEPVWTDVTGLPDRKFGFYGEQTFPVHIERPDHPIVRGLSDWTMFDETYTMQEPGEGC
                     DILLTTDYNPSMRALAWTRRHKQARVFCLQMGHDGRSWNIPSFKTVLQRGILWSAGKL
                     QG"
     gene            complement(20494..21426)
                     /locus_tag="PLS90_02285"
     CDS             complement(20494..21426)
                     /locus_tag="PLS90_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK01262.1"
                     /translation="MSPAAERPRRRRRHPLLHRLEFAALSGLLGLLGRIPAPLQRAFA
                     RGVGTLVYALLPIRRGVIQRNLARAFPEMSEAQRRRILRELHVHTILFALELAWLSRA
                     PHDAIRRRVIVSPADWERYQALARGNGHLFFCAHQGNWEWTGAWYALTFGRCGVIYKP
                     MHNPYADAWLRRLRERNALRTFSTREAVPRPLLRFLREGGCIGILADQDARHEGVFLP
                     LFGEVASTSTGPASLALRLNLPLQPVASYRQPDGRLLLEMGELIRPDPTAPREAEERR
                     IMSLYNAWVESTIRRAPEQYFWWHNRWKTRPPRG"
     gene            complement(21423..21821)
                     /locus_tag="PLS90_02290"
     CDS             complement(21423..21821)
                     /locus_tag="PLS90_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPK01263.1"
                     /translation="MMEAKILEFIQSRPVAVVGASADRAKYGNIVLRNLRDRGWTVYA
                     INPRAETIEGEPAYPNLAACPQTPGLAVMVTPPEVTRQVLDEAAAAGVGRIWMQPGAG
                     DAAAIARANELGLELIHDACIMVAAARHDR"
     gene            complement(21934..22356)
                     /locus_tag="PLS90_02295"
     CDS             complement(21934..22356)
                     /locus_tag="PLS90_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK01264.1"
                     /translation="MVHSPSSQPLRATVHQLDREQLWLLRGRLTHPVVDAFRNGVLQT
                     LAPAVERVYFDTSQLDHIDSAGLGILMALHRELRQRGGQLILLSPSAQQMNLLRGCRL
                     HLILTLVTGLEAEEHRGRLLRGETVELPHDAFLPPQLP"
     gene            complement(22463..23272)
                     /locus_tag="PLS90_02300"
     CDS             complement(22463..23272)
                     /locus_tag="PLS90_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK01265.1"
                     /translation="MPKEFEIDAYRHGRELLVILRGRLVLKYCQDTKTRLNSLFTPEV
                     DQVYIYLAELSFLDSAGLGVLVGTKMSSNKHRTRLALLSPQKRVEDIFRVSKLDAIFE
                     IRGGSEAELIRGNLQKDEYVVWRDSKEESESAYNLTQIDPQVNRDSAPLNPSPGSLGS
                     QATEPLIGADHDPAARVKQLCGDAVEYIRQGDYDKAVISYQQALDIDPKNLSALNNLG
                     IVYEKRPEWYTRAHEVWRMVLEVSRERSDEKHAARAEKHLESLEKLMHLKQ"
     gene            complement(23310..24941)
                     /locus_tag="PLS90_02305"
     CDS             complement(23310..24941)
                     /locus_tag="PLS90_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01266.1"
                     /translation="MTLGKREQAVIGSVGALVLILILHLAIFGPQAGAVAAAKSEYDT
                     KAATLQSLVMLTPQQKAENTLGTFNEQVGEMLARLREGITDLELEEPEAFTMPTIESI
                     QLDPPPPPGTTPDEISNLKNQELERLIQQRKQEQIDLLLAEVEHLRSFRENPTTRMNF
                     LGPGTRTYPGWNIPLELPDAMQTEALWDAVRRLSERKGTLEYLQPNSTAYFQARRIYD
                     AALAQVGLNVAHADGLDRYGEFVPLFYRLAVAGLILDRLPAERAIMIQQTPLNREMLL
                     DILDINLPDEELKGIKETKLYFAYMTIHNINDLLEKAIANNIEQVDAVYHRGFAYLRE
                     QPMGMGSPADTLPMNAVQLAQIDDEVDQKEPGEEEEMDMSAMGGMFPGMFPGMFPGMP
                     GMPGMPGGEGEGGAVPGGMPGMPGMFPGMPGMFPGMDGSMMGGMADPDAEPYKPKDDE
                     IGYAIPIKIVSTGSNRNTFNYLYNVLAQRPMVELHRLIVQSRPERIQAETTFLVVTKL
                     FDTIDVVRQILTEVEDTTTSPAASAALPAGAPAMP"
     gene            complement(25213..26181)
                     /locus_tag="PLS90_02310"
     CDS             complement(25213..26181)
                     /locus_tag="PLS90_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK01267.1"
                     /translation="MQCPQCHSVNANGALACTMCGAPLGRRLDTDPALPGAGADDDIY
                     VGPSYKLDSRAAHDPNELKRRSALASRFLLLATPLFLALMAGIQFRAEAAAYLRDMLQ
                     LEALTPTQFLLILAGTGIVLNAMLIYYSARAAGGNGLLAAAVALAPPLIPAGYALLAR
                     RRFWMPYVCLLVDGLILAAAWFIFTNYAWLTLVLFLLMIPVIAYHLSGCAAVDISLSL
                     GLLHYLSVAWVCLGPAVLAGWCLVEIGVMDLLANSLADGTHFPSLSEIGRQVYGQIGA
                     NPDRYQMALLVWLGVILLVWIKSVYENLKFPVIHESEQAEVWTTAA"
     assembly_gap    26361..26460
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26725..27729
                     /locus_tag="PLS90_02315"
     CDS             26725..27729
                     /locus_tag="PLS90_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01268.1"
                     /translation="MPLLEVQDLEVAFATDAGPLRAVDGVSFAVDRNRTLGLVGESGC
                     GKSVSALAVLRLIPNPPGRITAGRVLWKERDLLRLPARQLPAIRGAEIAMIFQDPMTS
                     LNPVFTIEQQLGEVLRIRFGQGRRQARARAREMLATVGIADPDRRLQNYPHELSGGMK
                     QRIMIAMALLAEPDLLIADEPTTALDVTIQAQILALIRELQQRTGMAMVLITHDLGVV
                     AGVCDEVAVMYAGRIVEHAPSAAIFENSQHPYTRGLLASIPRKGRRKESRLATIEGVV
                     PSLLEPPPGCRFAERCPRRRELPEADQRRCRQVDPELLPVAGTPNAAACHYPCSGERR
                     "
     gene            27757..28779
                     /locus_tag="PLS90_02320"
     CDS             27757..28779
                     /locus_tag="PLS90_02320"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HPK01269.1"
                     /translation="MTEPAAPPPPLLDVRDLRKHYPVRGGLFATQQATVKAVDGVSLR
                     VRPGETLGLVGESGCGKSTLGRTIPRLEDPTAGEVYFAGRNLAEARGRELFALRRQIQ
                     IIFQDPYASLNPRMTVGEIVREPLAVHRRGRRADQQRRVAELLELVGLSADVSDRYPH
                     EFSGGQRQRVGIARALTLEPKLIIADEPVSALDVSVQSQVLNLLVELQERLGLTYLFI
                     SHDLSVVEYLSDTIAVMYLGKIMEIGPARAVFEAPAHPYTRALIEAIPEPDPRRRVRH
                     APVRGETPSPINPPSGCVFHTRCPYAVEACRGVVPPLEAVDPARRGQADADDLHRAAC
                     IRKDEI"
     gene            complement(29142..30233)
                     /locus_tag="PLS90_02325"
     CDS             complement(29142..30233)
                     /locus_tag="PLS90_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPK01270.1"
                     /translation="MRIGLVGLQGSGKTTIFNALTGQQAQTSAFGGGRGGANQAVTKV
                     PDDRLDRLSALFNPKKHTPATVEFIDLAGVARGEGGAGDGLGDKQIAEIATAEALLVV
                     LRGFADAAGQPPDVTGDLEAINLEMALSDLKKVENRLERMGKSLRGAAGREKELLEIE
                     QAACVAVKGALEEGRPARAAALTPEQAKSLRGFQLLTMKPTMFLINEDEAGWAARKDS
                     PVDLGPASAWPDTYGARLCGEMEMEIARLDEADRAAFMEDLGIREPAAHRVIRLCYEL
                     LGQISFFTVGPDECRAWTVRRGALAPEAAGAIHSDLERGFIRAEVIGWEELLALGSYA
                     EAKKKGKLRLEGKQYEVRDGDVINVLFNV"
     gene            30541..>31232
                     /locus_tag="PLS90_02330"
     CDS             30541..>31232
                     /locus_tag="PLS90_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01271.1"
                     /translation="MKVTYNEQYERHLKTCFIGCGGHACRNIYPTFQYAPVDLAAVCD
                     LDAARAAHMARQFGARAHYTDHREMLDRERPEVVFIVTNYDKDAKPRYPQLACDAMRA
                     GAHVWIEKPPAGCLADVEMMMETSRKTGKWVMCGFKKMFFPANVRAHLLSRQEWFGAI
                     RTITARYPQHLPGPEERDDPWKLVGFLDHIGHPHSLLRLLGGPLESIYVERQRDGAAV
                     SARAGKATINVT"
     assembly_gap    31233..31242
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <31243..31678
                     /locus_tag="PLS90_02335"
     CDS             <31243..31678
                     /locus_tag="PLS90_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01272.1"
                     /translation="TLSANEDAVAPSGAVGTLHLCPGIGGGSPLEHTEIVGEHGNVIV
                     ENNIRVYCYSGGGPEGGYGVAGDFTDTGERGGAQFWEPEFSLGQLYNKGLFLLGYAPE
                     VISFCKHALAGTPPEKADLECIWEMTRIYEAYLQPTGKVIKI"
     gene            31953..32028
                     /locus_tag="PLS90_02340"
     tRNA            31953..32028
                     /locus_tag="PLS90_02340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31986..31988,aa:Gly,seq:gcc)
BASE COUNT         5105 a        10499 c        10988 g         5328 t
ORIGIN      
        1 ggatgttact ggatgtcttg aaaagaaact ttggtggagg cggcgggaat cgaacccgcg
       61 tccgaagtgt ctgccaataa agcgtctaca ggcttagtcg tcgctcagat ctcgcctggc
      121 gggcaggtgg acgaccgacc aaccagccag gccagctcga ttgatcttgc cgggcggccc
      181 tcgagcagcg ggtcacgcgg ccagtccgct gggtgacgcc cattccgaag ccacggacaa
      241 gcctcggatg gacgggtcgc ctagattagg cggccagggc gagttcaggt tcgccagtta
      301 ttgttagctg tcaagtctga ttaacgaggg gactcaacga acctcgacct gcaactctat
      361 cctcagtcca ctccgtcgaa accttaacgc ccccacaatg gcgttcgttt cctgttgtcg
      421 aagaacgggc ggcggggccg aaacgcgggc gccccgccac cacgatggag agtatagcac
      481 ggaggcggcg aaaagggaag ggggcgcgcg gacggcgaca ggcgggaaga aggacacggg
      541 aggggagctg gatcacgaaa ggcgcgaaag gcacaaaagg cacaaaagag tgaaaaccca
      601 agttgcttcc ccgccaagtg aggattgact ttcgagagcc gaggacgaga gccgggagcc
      661 gaatcattga gcagggacgc gcgaggacgg gggacgggcg cctgcggacg gtaagtttcc
      721 tcgcgagtcg ccggcgggat ctgctacaac aggcttggga attggttcgg gccggaatcg
      781 gcgacgggtg cttggaatgc cgccgggcgg acgctttccg ccgccccccg ccgaccggtc
      841 gccgccgccc gttcccggtt caccccgcgt tctccaaccg gccgcgcgcc cgccgcgccg
      901 ccgccccgcg caggagtctg ctccgccgtg cccagccccc gcctgctgat cgccgaggat
      961 gaagagcgcc tgcgccgtct gctgggcatg ttgatggcca acgccggtta caagctgacg
     1021 atggtcgccg acggccagga ggcgcgcgag cagttggatg cgcacgattt cgacctgatc
     1081 atcaccgaca tcaagatgcc gcgcatggac gggatgcagt tgctggccta cgtgcgcgag
     1141 cacaagccgg atatcccggt ggtcgtgatc acggcctacg gctcgatcga atcggccgtc
     1201 gaggcgatgc aggcgggcgc gagcgattac ctgaccaagc cgttcgagga ggaaaagctc
     1261 aagctggcgg tcgaacgcgg gctgcgcatc cgcaagatcc tggccgagaa ccgccaattg
     1321 cgccgcgagg tggacagccg ctgggggctg gaccagatca tcgcccactc gccggcgatg
     1381 cgggaagcga tcgcgatggc gcgccaggtc gcccagagca acgccaatgt gatcgtgtac
     1441 ggcgaaagcg ggacgggcaa ggaactgatc acgcggggga ttcacaacgc cggtccgcgg
     1501 gccagcgggc cgttcgtcgc gatcaattgc gcggccattc ccgacaacct gctcgaaagc
     1561 gagctgttcg gccacgagaa ggggtcgttc accggggcga tcgaacgcaa gccgggcaag
     1621 ttcgagctgg ccgcgggcgg cacattgttc ctcgacgaga tcggcgacct gtcgctgcac
     1681 gtccaggcca aggtcctgcg cgcgatcgag aaccgcgaaa ttcagcgggt cggcggtacc
     1741 gaggtcctcg aggtggacgt gcggttcatt gccgccacga acaaggacct gcaggccgag
     1801 gcggcggcgg gccggttccg cgaggacctg ttcttccggc tcaacgtctt cccgatctat
     1861 ctggcgccgc tgcgcaaccg ccgcgaggac atcctgccgc tggtcaacct gttcctcaat
     1921 cggttctgcg cgcagatggg caagcgcgtg ccgcggatcc cgtccgagac gcgcgatcgc
     1981 ctgctggccg acccctggga aggcaacgtg cgcgagctgc agaacacgat cgagcgggcg
     2041 gtgatcctgc tgcagggcga cgtgctggcg cccgagctga tccgcaagat cagcgggccg
     2101 agcttgcgcg gggcggagac cgcctcgacg cggtttgaat tgccgcccga ggggttctcg
     2161 ctggaggagc atgagcagga gctgatccgc caggcgctgc agcgcagccg gttcaagaag
     2221 gcggccgccg cgcggctgct gggcctgtcg cgcgccacgc tgcgctaccg gatggagaaa
     2281 tacgggattc ccgacaaacc ggcgccgccg ggctgagggc tcgtttctgg cgcgccgggg
     2341 cgactcaatc cgtcggcagt tccttgatgt cctcgccgac ttcctcgccg atgcgcaacc
     2401 gttcctcgat gaccggctga ttcggctcca ggttggacgg ctcctcgccc ggcatgtcga
     2461 tcaggcccgc ttcctccagc cagaggccgt tgtccttgac gttgtcggcc gtgaagaacc
     2521 gcgagggcag cacgatctcc ttgggcgcct ccttgccgag caggatctcg acggccgtgg
     2581 cgatcgcttc ccggccgccg gtgggatact cgaagctggc gtcgagaatc ccttcctcga
     2641 catagcgttt gccttcgtgg ggcagggcat cgatgccgac aaagatcatc tcgtcggcgc
     2701 gtccggccgc acgcgcggcc aggtaggcgc catgcgccgc cgggtcgtta tggccgtaga
     2761 ccacatcgat atcgggaaag cgcgccaggg ccgactccat ttcctggcgg gctttcggct
     2821 cgagccattc catgtcggcc tcaaagatga ccttcagatc ggtcccctca atccccttca
     2881 ggaagccgct gtggcggtcc tggccggggg tgctggtcat caggcccttc agttcgacga
     2941 tgttgcccgt gccgccggtc tggcgggcga tccagcgccc gaccgcttgc ccgatcagct
     3001 tgttgtcggc gccgatgaac gtcgtgtact gatcgcccat gatgcgccgg tcgagcacca
     3061 tcaccggaat gccggcctgg taggcgcggg ccaccggttc agtcagcgga acggcttcct
     3121 tggggctgac gagcagcaca tcgaccccct gattgacgaa ctcctcgatg tgctggcgct
     3181 ggaccagcgt gtcgttctgg gcgtccttga agatcgcccg gacctcggga tgggcggccg
     3241 ccgcgtcggc gatatccttg ttcatctgcg cgcgccaggg ctcggccagg ttgcactggc
     3301 tcatgccgat gatccaggga tcctccgggg tgccggaacc ggcgcgcgcc ggcgccgctc
     3361 ccccgatgca gagaccgacc aggagcgcca gcagaattgc ggatcgaaat gacttcatgg
     3421 ctttacacct cgcgttaacc ggatgagccg gcgcgccgct ccgaggccag cgccggcatg
     3481 ggacggacca tttgttagca ggttccgtgc gcgcggcgaa tccccctccc cgggcgattc
     3541 tccccgacca gcagggctat tttcgggtgc gggcggcgga tttgcaaggc ttcgcatggc
     3601 atcggatcca aagcggtgcc gacgcgttgc gcggcaacgc tcgggtaccg cactccacga
     3661 aagctgcctt gcccccgttc catgccccag atcaagcgct cgcgtggcgg acggtgttct
     3721 tcccggcggc cgtgggtgcg gcccttccgc gaaaaaacgc cggcgcgggg tgattgtgag
     3781 gctgaaacgg gcgggaattg cggatatgat gggggttcat tgcgcgcgcc caggggcgcc
     3841 agacggcgcg cgcccgcccc ttgattcggg acgaaaggcg agttcccccc accccatggc
     3901 ccggttggac tggctccagc gactgcgtcc gcggcgattt ccgcgccggt cgcacgcgcg
     3961 cttcaatcga atcgcgcgcc gctggctctt tgccggtctt tccggtctgc ttgcgctgta
     4021 tctattcacg ttgctgttcg acatggagcc cttcctgatc cggacggaac tgggcctgtt
     4081 ccaatggctg gacgggcatc tggggaaaga cagcgtcctg gattggacag tgctggttgg
     4141 cgcgagccgg ttctgggttt atacgtcgct cgccgtggtg ctgtttgcgg cgttggcgca
     4201 ggggtgggcc atgcgccggc gcgactatgg ccaggtgttc gggctcattt tcttcgccgc
     4261 cgtagtaacg attctcgcgg aggaggtggg cgacttcgtc agcgaccaca tcaaccgcga
     4321 cctgccgtgg gacagcggcg cgctggaaac gatccgcgat cgctacgacg ccgactttct
     4381 ggacatcccc tccaccggcg gcattgtcga cgagaacatg gtgggctggg gggtgatgat
     4441 cctgctgttg tcgctgcggc tgccccggcc ggcgtggatc gccctggcgc tgggcctggt
     4501 gcatgcgggc gcgcggctca ccgtgggggc gcagtggctg ttcgacgagg tgaattccgc
     4561 cttgctgagc ctgacgctgg cggggggcgc gctgctgggc ctggggcgcg cgctgcgctg
     4621 gtgcgaacgc aatgcctccg agcagatcat cctgttgacc ggatttcatc tggcgccgcc
     4681 cgaggcgggc cacgccgccg tccgggcgga attgacgcac aacgtggccg aggcccggcg
     4741 gctccaccgc ctgcgccgcc atcggttctg ggagaacctg acgcggcgat cggtcttgcc
     4801 ggtgctcaac gcgacggggc ggccggccac gctggcggcc caaccgcccg gccgcacgat
     4861 tccggggttc cggcccagcc cgcacgtccg ctttctggcg ctggaggggg gcgagctgtt
     4921 cgtgatccgg gcaatgcgcc gctggggcgg gtggctggcg ccgtcggcca aattccgccg
     4981 ctacgtgcag agcgcccggt gtcacctgta tctggagcgg ctcgggttgc ccgtgccgcg
     5041 gatgttctgg gtgcgcgaga cgctgacgac gctcgggctg cgcaatctgt atttcctggt
     5101 cgaggagttc atcgatggtc ggccggtgga tcgcgacagt caggccgagt tgcgcgcggc
     5161 cgtcgggctg ctggtccggc ttcatgggtt tgagtcggag acgtggggcc cggtggcgca
     5221 ggcagccccg caaccgcggt cgcactatct gctgcgcgcg ctccgcccgg atctgctcta
     5281 ttacctgcgt cggatcgaga aatgggccga cgtccgcttt gatcacgacg acctggagcg
     5341 gctgtggaag ctggaggagg cggcggcgct ggccgtgttg ctgcgccggc gccgcccgtt
     5401 ccggctgatc cacggcgacg tgcattaccg gaacttcctt tacggccccg cgcggggcga
     5461 agtgcacctg atcgatttca cgacggtggc ctacgacttg cacgggtggg aaatcctgaa
     5521 gatggcggcg gcgttcgggc ggcgcgaggc gccggcggcg gcacggctct ggcgggaata
     5581 cttcgaactg gccagcgaag aacgttgggg cgagtttctg cacgaggcgc cgctggcgct
     5641 cttccgccac ggcctgcgcg agatggctca ccagcgcgtc gcgccgccgc gcgccccggc
     5701 cgccctgcgc aaggcatcga tcctggagtg gctcgcgggt ctgctggcgc cggcgcccga
     5761 actgtggggg gcgcgcgcgg cggagaccga ctgggaggcg ctgctcgagc gcatgcggcc
     5821 cgacctgtgc gtcgcggccg gagtccaacc gcccgccgat tcgtcggtgc tcccgtaacg
     5881 gcaacggccg gcgctcagcg ccgggggatt gcctccgcgc ttccaatacc gattccggtt
     5941 tcgattccga taacgatacc gatgccatcg gttggcgttg ccgatggtaa gttgttcacg
     6001 aaatcagcgc gcgacggccg ccgccggacg cctcgctcac gctatcccgc ctgctttcag
     6061 cgctccggga caaccctgaa aacggcctgc cgattgggaa cagctcttgc ctcaagggtg
     6121 gggcaacaga gtcaaggatt accccgaagg agccgtcacc atgcatcccg aaaatcgccc
     6181 ccccgttcga accatcgcgc gcgaagcccg cggcaacgac ccgcggatcg tcctcttcat
     6241 cgccctgttt ctcgccctgt tcaccatgct ggtggtggtg ggtcaggccc agcaggcgga
     6301 accgccgagc gacgcggcca tccaacgcac cacgacgatt gagcagacga caattacccg
     6361 gcgagacggc gcgcccccgc tcgaactgag cgacctgggg atgaagctgg cgatcgaacg
     6421 cgagctggcc cgcgcgccgg ggatcaatac cgcgacgctc gacatcgacg tcagcgacgg
     6481 gatcgccacg atcaccggct cggcgccgaa cgtcatcgtg cgcgaccgcg tggagcggat
     6541 cgccgcgacc gtgcgcggcg tgaccgccgt ggtgaaccac ctgacgatcg agtcggccga
     6601 tgtggccgac ccggtcatcc tggcaaacct ccagcgcgcg ctgggcgccg acccggccct
     6661 cgatccggga ttactccaga tttcgatcga ctcggggatt gtcacgctga gcggatcggt
     6721 cgatagctgg cgggaacgct ggctcgccga ggaggccgcc aagaacgtct acggcgtccg
     6781 gggggtcgtt aatcagattc aactcgaata ccccgagggg accgccgggc gcgccgatga
     6841 cgagatccag caacagatta tcgatcgcat caacggcagc gcgctgatcg acgccggcct
     6901 ggtcgccgtg ggcgtgcgcg atgggcaggt gtcgctgtcg ggaatcgtcc ccagtctggc
     6961 cgataaagtg acggcgatgc ggctggcgtg ggtcagcggc gtgaccagcg tgacggccga
     7021 cgagttgaaa gtcgactggg agctgtaccg gacgcagtac acgccgccgg ccgcgccgag
     7081 cgacgcgccg gtgcgttcgg attcggtgcg cgagcaggcc cgcgatgccc tgatgctgaa
     7141 cccgtacgtg gagcaggaga acgtcgaggt ggtgattaat aacggcgtcg cgacgctctc
     7201 gggaacggtg cggtcgctga ccgcaaagcg cgaagcggcc gaagcggtgc gccatgtggg
     7261 cggcatcttc gaggtccgaa acaacctgat gatcgacccc ggaatcgagg caaccgatgc
     7321 ggcgatcgcc cagagcgtga gcgacgcgct gcgcgtggac ccggtcgtgg aacacgtcga
     7381 ggtggatgtg gcggtcgacc aggggatcgt cacactgcgc ggccaggtcg atgacgcgtt
     7441 cgaacgcgcc cgcgctgagg aggttgccgg ccggatcgcc ggcgccgtgg cggtggcgaa
     7501 tgagctgacg atcgcccagc cggaagcggt cgccacgccg agcgccgcgc ggagcgatgc
     7561 ccagatcgcg gctgatattg aaagcgagct gttctggagc gcgctggtcg acccgagcga
     7621 cgtgcaggtt caggtccagg acggcgtggc gacgttgcgc ggccgggtcg acgacagccg
     7681 tgaatacggc gccgcgattc gcaatgcgtt tgagggcggc gcccgcagcg tcgtcagtga
     7741 gttgacgacg accaacgggc cggtcggccc ggccacccac ggcgagccga tcgtctatga
     7801 ttcgccgttg atgagtgagt tccgctgatt aactggaatc gggatcggta tcgccatcgc
     7861 aagcgaacca agagccgaat tcttgagtca tgtcagtgcc ggtcctgctc tttcagccgc
     7921 gattcggccc agtcggcctt gcgggcgaga tagagcagtt tcctggggag cgtggcgtag
     7981 cgcagatcgc gcagcgaccg gcgcagtcgc atgatcagca tccgccgctc gcgatgctgg
     8041 atgacggcaa aggcgccgcg cgagagcgag gcgttggcct ccagcgccca ctgatacgtg
     8101 tcggggtcgg cttcgccgca gagcgaatag aacatcgcga cgggctgacc ggaactgtcg
     8161 gcgcgggcgg cgtcgaggaa gattcgctgc cggcgggcgt cgccgacggg caggacgcag
     8221 gcggcgccgg cttcacgccg ctcgaacggg ccgccctgtt cggccgtcca ctgctccagc
     8281 cagcggatca ggtcttcggc gtcgcgcggt ccgtcgtcgc gcgcatcgcg cggcggcgcg
     8341 gcggccggcc cggtcgcggc gaccggcgtt gcgcccgttt cgtcgacgac atcgcggctg
     8401 agcgccgcgt caagcgtcgc cgccagttcc tccacgtccg aatcgagatc gcggcggatc
     8461 tgcgtcaggt attgctcgcc ccagtgcttg acgtcggggt cgctgtcgcg ggcggcgcgg
     8521 cgcagttgca gcagcgcctg gcgggcggtc aggtgcagca ggtcgcaggc gtcgcgccgc
     8581 tcggtccagt acgcggcgcg cgtcagatgg gccacgacct cgcgcagccc gctccacaac
     8641 gattgagcgc cgggggcagc catgagacgg ttccctttct cggacccctc ccgtccgccg
     8701 ggactgcatg gcgcgggcgg cggcgtcgcg cggctcagcc ggtcatatcc tcgtcggcca
     8761 tttcgggctc gtcatccacg ttcggatcga acagcgccga caccgagaga tagttgtgaa
     8821 tccggtgaat tgcctcggcg atcaggggag cgatattgac cactttcaac ttggcgagcg
     8881 tctggtcgtt gcgctggggg atcgaattgg tcacgacgat gcgctcgagc ggcgcctcct
     8941 gcaggcgctg gcgcgccggc cccgacagga ccgggtgcgt cgcgcaggcc gaaaccgaca
     9001 gcgcccccgc ctcgcgcagc gcatgcgccg cctggcagac gctgccggcc gtgtcgatca
     9061 tgtcgtcgac cagcaggcag tggcgtccct tgacctcgcc gatgatgttc atcacctcgc
     9121 agacgttggc cgccggccgc cgcttgtcga tgatcgcgag cgaggcgttg agccgctggg
     9181 cataactccg gcacatcttg acgttgccca cgtcgggcga gacgactacc agatcgcgcg
     9241 cccactcctg gcgctgcaga aacttggtga tgacggggcc ggcgtagaga tgatcgacga
     9301 cgatgtcgaa gaacccctgg atctgcgccg tgtggaggtc caggcagatc acgcggtcga
     9361 cgccggcggt ggcgatcagg ttggccacca gtttggccga gagcgccacg cgcccctgat
     9421 ccttgcggtc ctgccgcgcg tagccgaagt agggcatgat gacattgatg cgggcggccg
     9481 aggcgcgccg catcgcatcg accgtcagca gcagctcgat caggttctca ttggtcggcg
     9541 ggcaggtggg ctgaatcagg tagcagtcgg cgccgcggac gttctcgcag atcttgacgc
     9601 cggtctcgcc gtcggagaag cgcgtcacct cgagctggcc ctcgtggagc cccagccgct
     9661 tgcagacttc gcgcgagaga tgcttgccgg ccgagccgcc aaagaccagg ggtccggaat
     9721 acgtggggtg aatgcgggcc atcggaaact ccacctcggt tgcgcaccgg gtcaaaagcc
     9781 caagctacca gccggccggc cattaatcca gcggaagtgc ccgggggcgt cgagggcgcc
     9841 gcgccccgcc cgggggattg caaggcgggc ccgattgtgc caaggttctg ctggggaccc
     9901 ggttgggtcg tttcatcacc aggcccttcg ctcggcgcga gcgtgaacgt atcgggcgcg
     9961 cccgacccgc caacgcgccc atcgccccgc ctgaccggtc cacccccgtt ggaagtccac
    10021 gatcgggaat gcgtcgccgc aggccgagat gatgtctgaa ccgcagggcg aacgcgccca
    10081 ggcttatccc ccgttgaaga tccgcgtgct gggatcgggc acgtcgatgg gcgtgccggt
    10141 cgtcggctgc cgctgcgcgg tgtgcacctc gagcgatccg cgcaacaagc ggatgcgctc
    10201 gagcatctcg gtgcaggccg gcgacacgtg gatcctgatc gattgctcga tcgatttccg
    10261 gatgcagttg ctgacctggc cgatgccgcg catcgatgcg ctgctggtga cgcacacgca
    10321 ttcggatcac atcaacggca tcgacgatct gcgctgctac aattacctgc agcggggccc
    10381 gatcccgctc tactcgaccg agtatttcct caacgatctg cgcgtccggt tcccctactg
    10441 tttcaacccg ctgcagcgcg gcgggggggt gccggaggtc gacctgatcg gggtggagcc
    10501 ggggcggccg ttcgattgcg gcggggtgcg catcctgccg ctggcgatca tgcacggccg
    10561 gctgtcgatc ctcggtttcc gattcggccc gttcgcctac atgaccgatt gcagcgcgat
    10621 ccccgaggag acgctggcga agctggccgg cgtgcgcgtc gtgatcctgt cggcgctgcg
    10681 ccacacgccg cacccgacgc atttcacggt ggagcaggcg gtcgaagcgg cgcggctgat
    10741 cggcgtcgag caggcgtatt ttactcacat cgccgatgaa ctggatcacg aggcgaccaa
    10801 tcgcaaactg ccgccgtggg cccggttggc ctatgacggt caactgatcg agctggccgc
    10861 gacgggcggc gcctgatggg tcggcggcgt cgcaagccgg cggcgggtca ggcgggacgc
    10921 cgcgcggcgc cagcggccgc cgcggccgca ccgggggttg agccgcgccc gtcgcggcgc
    10981 gatctcccct ggcgcaatgc ggcgcgcgac ccgtggctct ggggcctgct ggcgctggcc
    11041 gcgctgctgc gccttccgtc gctcggcgtg cccagcctgt ggtttgacga aatccgcacg
    11101 ctctacatgg cgcgcgacag catcctcaac gtccagagtc acttcggctt ctggtgggtg
    11161 acgctggcgt tcctgcagtt gccgctcagc acggatatcg cgttgcggct gttcccggcg
    11221 ctgtgcggcg tggcgggcgt gccggtcagc tatctgatcg gctacgcgct gcggggccac
    11281 cgggtggccc gctggtgcgc ggcgatcgtc gcgctcagcg tgtttcatct gttctacagc
    11341 caggaggcgc gcttctacgc gccgatgtat ctgctgacgg cgctctcctg gctgttcacg
    11401 atccggctgt tcgcgcggag cgcaccggcc tggtttttcg ccgccgccgc ctgcgacgtg
    11461 gcggcctaca cgatgcaccc gacgcagacg gtttacctgg ggctgcacct ggcctacgtg
    11521 atcggggcgc tggcgctgac gcggccgggg cacgcgatca cgcgggagta tttcatgccc
    11581 gtccgcgcgc ggagcgaggg cggggggatc aacgccgcct cgacgatccg gctggttgtc
    11641 gccgccgcga cggtgatcgg cagcgcctgg tatgcgcggc ggtttctgag ccgcatcacg
    11701 cagatcaact tcgatccgga gtatctgatc ggcgggctga cgccgcgtct cgtggggcgg
    11761 cacgtgcagg cgttcggcgg gcagtttcag ctctgggatc tgcccggcgg gctctacgcc
    11821 tggctctatc tggcgatgct gctcgtgggg ctgtgcgtgg cctggcgccg ctggcgtcgc
    11881 gattgcgtct tcgccgtgat gctgacgggc ggcacgttcg tcgccagccg cggctacgag
    11941 gtttcgatcg actacgccgt caagtatgtg atgttcctga tgccgttctg ggcggcgctg
    12001 gcggcgcagg gcttcgcctg gaccgcgcgc gtcggcggcg accggctcgc ggcccggctg
    12061 ccccgcctcg cggcccgcgc ccggctggcg ccgacctgcg cgctcgtgct gatcatggcg
    12121 gtcgcctcgg cctttgcctg cttcggctac tacagccggg ggaagattcg ggagaaggcg
    12181 gccgtccggt ggctcaggga ccgcttgcag ccggcggacg aggtgatggt ctatggccac
    12241 agcgcgctgc cgatcaattt ttactggcgc aaatttggcc ttcccgccga ccagtatcac
    12301 gcgctgtggt gggaacccaa cgaactgggc ggcaatctgt tcgggtcgat ggaggcgctg
    12361 cgccggccgg gtgggaaggt ctatttcatc gattcgtggg acaaggacga cccgccgcgg
    12421 atggccgaac tgctggcgca gcgggggcga atgctgcgcg agtggccgtc gttcatgggt
    12481 cagccggtcc gggtctatga gattgtgccg cgcgccgccc cggcgggctg gccggttcag
    12541 ctcgacccgc tggggccgcg gatcacccgc aatccctcgc ggccgtacca aggagaggtc
    12601 gccggtcagg aggcgctgat tctgcgcgac aacagcacgg tgctctacga cttcacgctg
    12661 gccgagggcg ggccctgcca cctccagctc caggccaacg ccgggccggt tcaggtccgc
    12721 atcatcgaag tactggtcga cggtcggctg caggggatgg tgtcgtttct cgcgcaagag
    12781 cccgcctggc tgacgcgcca tgtcgacctg ggcgagctgg ggccgggcga gcatgtcctc
    12841 acgctgcgct atctgtcgcg cagcggtcgg ctggagctgg agggggccac ggtggaaggg
    12901 gtcgcgctgc ggagcctctc gattcatccc ggccctccgc ccgttgggga tagcgacctg
    12961 gtccggctgg atttcatgga cgcgaactac ccgagtctgg cgctgcgcgc cgatgacggg
    13021 acgaccggag acttaaaccc ggattggcag cccttgaatc tgcccgattg ggaactggtg
    13081 cgcgttccgg cggtcgatgc gccgacgacc gacggcctga ccgatgccgt tctcatcacc
    13141 atgccgcccg aattcgacgg gttcgcccgg ctgttgttcg accgcgcctt cccgctcacg
    13201 gagcagcgcg tggcgtattt tgccttcgat gtccgcaccg agcggttgta caaccactcg
    13261 atcaacgtgg gcttcctctt tctggatgaa tcgaacgaac cggtactgca acatatggcc
    13321 tattcgcacg ggctctttga cgacatgaac tgcaccggct atctgggcga ggaatggacg
    13381 gagggttggg tgccgatggc ggcgatcgat ttcgccccgg ccggcgccat gtggttctcg
    13441 ccgttcattg tgctgtacga caacccggtc cggcccagcc gccacagcgg acgggtgtgg
    13501 atcaaggatc cccgcctgca gccaatcgac accgccccgc ccgtgcgcgc cgcggcgcca
    13561 tgaccgcgtt tttgctcacg ttcggcggat tcacgttcgc catgctggcg gcgtttctgg
    13621 tggcgcgcgg cgtgaccggc tgggcgcgcg cgctggcgtt gcggtgcggc gtcatcagcc
    13681 acgtcgatca ccgcagctcg cacacgcggc cgacgccgcg gctgggcggc ctgggcctgg
    13741 ccgccggctt cgctctgccg gcgctcggct ttctgctctt gctctggttc attccgcacc
    13801 ggggcgcggc gatgggctat caccccgcgc cactgctgtg ggttgggctg ggctggctgc
    13861 tgatgctggc ggtggggctg gtcgacgacg cgcgcaacct gccgccgctg gtcaaactgg
    13921 cgctgacggc cctggccgcg ttggccccgg tcgcgggcgg gggcgtcagc ttcacgtttg
    13981 cccaggggtt tttcttcagc acggcggcct ggtcggtcgc tctggcgctc ctcagcgctg
    14041 gctggatcct gtttttcacc cacggcttca acttcatgga tgggatggac ggcttcgcgg
    14101 cgaatttcgc gcgcgtggcg gcgctgggaa tcttcggcgt cgcgttcgtc ccctgcctgc
    14161 tgtcgggcaa catgaacctg ctgcgcgccg aggcgtatct gctgccgatc ctggcgatgg
    14221 cctgctgggg ctttctgcat tggaaccgcc cgccggcccg cgtcttcatg ggcgacggcg
    14281 gcagcctctc gaccggctac ctgctggcga catggaccgt gatggggcat cgcgggatgc
    14341 tggggtttcc cgtcccgctg ccgctggcgc tggcgatcct gatgccgttt ctgttcgatg
    14401 tgctgctgac gctggcgcgc cggctgcatc ggggcgagaa cctgctgcgg gcccaccgcg
    14461 agcacctgta tcagcgcctg ctgcggaccg gcctgggcca cggcgaggtg ttgcggctga
    14521 acctgccgcg gttcgtcggc tgcgccgcgc cggccgtgct gacggccagc ctggcggccg
    14581 tcctcaactc ggggtgggtg cgcgggcggt tcggcgaggg gccgatcgcg cggatccatg
    14641 cgccattcgt gctgccggcg ctgcacctgc tggcgctggg cgccgccctg ctgatcatgg
    14701 tgcaatactg gtgcatggtg cggcggcggg aggcgcgctc cggggcggag gaggccgccg
    14761 gcggatgacg aaaaaaaagc gaaagcgcga cccattttcc accttgcatt gaccccggtg
    14821 ctctggctta atgaaccgcg tgatgccgcg tcgggcgcct tcatccgctc atcgttgtct
    14881 tgaatcatcg acgcacgggc cggggtgccc cacgccatcc ggatagccga cctcgccccc
    14941 ggaaaaggtt aattcattga ccatgatgga tcaggttcaa cagcttgagg aacgcgtcga
    15001 ggccatcatg cgcctgcttg acgccgcgcg gcaggccaac tcccgtctgg aacaggaaaa
    15061 cagcgaactg cgcgaacagc tcgcccggat ggaggagctc gattcgacca accaccagct
    15121 tcggcggcag gtcgaaacgc tggagaacga aatggagggg atggccagca aggagacgca
    15181 aatccgcgat cggctgcgca cgattctgga gcggatcgac acgctggaac acgacgtcaa
    15241 cgcgcagatc ggccagcccg acgtcttcgc caactccaac ggccagccca atgaagagca
    15301 ggcgggctga cgccggccgg ccccgggcca cccatgcatc ccatcaccat caccatcgcc
    15361 gaccagagct tccgcgtgct ggtggaggag ggggaggaga agcgttatga gcgcgttcag
    15421 caactggccg acgaggcgta tcacgagatc ctgcgaaccg gcgccaacgc cgggctgcgg
    15481 gcgatggcga tggcgctgtt ccaggtggcg atggacctgg acgaggcgcg ccagttgctg
    15541 cgcaaggccg aatcgagccg cgatcggctg agcgacctga tccggcggat cgaccgggcg
    15601 acggaacgct cggcccacgc cgccccgccg ccggcgcccg accgcgccga ataagccccg
    15661 cccgcgccgg tcgaacccct cggccgccgc caatcgaacg ccgccgcccc ccgtgcatga
    15721 ccggaggaac tcccgtggcc aagaccgccg tcaaatccga aaccaacgac aagctgaaga
    15781 tgctggacgt cgccctgagc cagatcgaga agaactacgg caagggctcg atcatgaaat
    15841 tcggcgactg gcatgcggag aacatcccgg ccatcagcac cggctccatc tcgctggacc
    15901 tggcgctggg cgtcggcggc atcccgcgcg gccggatcgt cgagattttc ggccccgaat
    15961 cctccggcaa gacgaccctc gccctgcacg tcgtcgccaa tgcccagaag gaagggggca
    16021 tcgcggccta catcgacgcc gagcatgcca tcgatccgat ctacacgcgc aagctggggg
    16081 tcaacctcga tgagctggtg atcagccaac ccgactacgg cgagcaggcg ctcgacatcg
    16141 ccgagcagct cgtccgttcc aactcggtcg acgtgatcgt cgtcgattcg gtggcggcgc
    16201 tggtgcccaa ggcggagatc gagggggaga tgggcgacag cttcgtcggc ctgcaggcgc
    16261 ggctgatgag ccaggcgttg cgcaagctga ccggcgccgt cgcgcgctcg aagacctcgc
    16321 tgatcttcat caatcagatc cgcgagaaga tcggcgtgat gttcggcagc cccgaaacga
    16381 cgaccggcgg ccgcgcgctg aagttctaca gctcgattcg gctggatatc cgccgcatcg
    16441 ccagcctgaa ggacggcgac cagaatatcg gcaaccgcgt gcgcgtcaag gtggtcaaga
    16501 acaagtgcgc cccgccgttc cgccagaccg aattcgacat cctgttcggc gaggggatca
    16561 gttatgaagg cgatctgctc gacctggccg tcgcgcacaa ggtgatcgac aagtcgggcg
    16621 cctggttcag tttccagaac gagcgcatcg gccagggccg cgagaacgcg cgtcagttcc
    16681 tcaagaccaa ccccgacgtc accaaacgcg tcgacgcgat cgtccgcgag cacgcgatgc
    16741 cctcgttcgg cgaggagagc gagacgccgg tgagcgtcga ggattaaggc cgcgcccggc
    16801 cgggcccgcc gccgcgactt ccccccgaaa ggttcatgac atcttgctgc gagtcgacgg
    16861 ccgccagccc gatcagatgc gcgcggtgaa catcacgccg cattacatgg agtatgccga
    16921 agggtcctgc ctgatcgagg tgggcaaaac gcgcgtgatc tgcaccgcga cgattgaaaa
    16981 ccgcgtgccg ccgcacgtct tcgggaccgg ccgcggctgg gtcacggccg agtatgcgat
    17041 gctgccgcgc tcctcgcagc agcggatcgt gcgcgatggg atccgcggca aggtcggcgg
    17101 ccgctcgcac gagatccagc gcctgatcgg ccgcgcgatc cgttcggtct tcgatctgga
    17161 tcgattcggg gaacggacgg tgctgatcga ctgcgacgtg atcacggccg acggcggcac
    17221 gcgctgcgcc tcgatcacgg gggcgctggt ggccgtgggc ctggcgatgg agcgcctgcg
    17281 cgccgagaag aagctttcca ttcagccgct caaggcctac ctggcggcgg tcagcgtcgg
    17341 cgtcgtggaa gggcagccgg tgctcgacct gtgctacctg gaggatgccc aggccgaagt
    17401 cgatatgaac gtggtgttga ccggtcggcg cgaatttgtt gaaattcagg gcacggcgga
    17461 aaccaggccg ttcgacatgg cgacgctcct ggagctgaac cgcctggccg gcgaaggcgt
    17521 cgggcgtctc gtcgaacttc agcacgagat cctcggcctg gacctggaac gcagttgaag
    17581 cacggccgcc gccgcgccgg ccgccgcgcg ccgccagtct tccccgcgaa ggaacgcctg
    17641 tttgcatgtt tcccgttgcc gttgttcccg ctgaccgccg gcgcctcggg ccgttgggcg
    17701 caatgacgcg cgcctgcgcc tgggcgctgg ccggctgcct gctgctggcc ggttgcgccc
    17761 gcgagccgct gatccgcgac cggcgcgtga tggtgatgga ttttgaaaat gcgatcgacg
    17821 atccggcgca gagtcacctc tcgggcggtc tggccgagat gatgacctcc cagctcgtca
    17881 attacccgcg cgtcgccgtg atcgagcgcc aggacgtggc agcctacttc gataaggccc
    17941 gcaccgaccc gcaacgctgg cacgaactgg gccgcaaatc gaacatcgat tacttcatca
    18001 tcggctcgat cgcccggctc gaggacaact acgtgctcaa cgcccggatc ctctcggtgg
    18061 cgaccggcca gatcgtcagc gggtcgtcgg tgacccgttc ctgcgcccgc gaggaggata
    18121 tttacccgac gatgcaggcg gtgacgcgcg ccatcgcgcg caacattcag gtgctggccg
    18181 agcgattcga ccggctcgcc accggcggcg aggcggccgt cgccaccgcc caatgacccg
    18241 gcgcgacgcc cgtctccgcc cggtccgatg ccgttctggt ctgaaaggga cgccttgatg
    18301 tcacgtgtgg gtcaaccgct cgctgcccgc cgcgcgggcc gcctgctggc gctgctggcg
    18361 ctgctggcgc tgcccgcggc gctgaccggc tgcatccgtt cgcgggtctt catcacctcc
    18421 gagccgcccg gcgccgaggt gttgtggcgc ggtgagccct acggcgccac gccgatcgaa
    18481 atcccctacc tctggtactg gcattacgat tacgcgctgg agaagcccgg ctacgaacga
    18541 atcgacgtcg tcgaccggtt ccgcacgccc ccctggttcc tgatgcccgt cgatctgttc
    18601 atggagatgc tgccgattcc catccacgac acgcgcgaac ggcacttcgt gctgacgccg
    18661 cgagaaccga cgctctcgca acttccttga ccgacgggga cttttcctgc cgggaattgt
    18721 gactcaaccg cgctgatgcg accccgccga accgtcatcc tcccgccgcc ggcgcgcctg
    18781 gcgacgctcg tcgggttggt cctgctcgcg gcggcgaccg ccggggcgct gccgaccgat
    18841 gccatcgtcg cccgggtcaa cggcacgccg atcctgctca gcgagctgaa ggaggcggcg
    18901 ctgaaccagg atatcccgat cgacgccctg acgctggacg gcctgcgcgg cgaaaaatac
    18961 cggctggtgc tgacgcaact ggtcgatgag gaattgctcg tccaggaagc gcgccagagc
    19021 gaaatcaagg ttgacgacgt ggaaattgcg cggctcgtcg acgcaatgat caaccagttg
    19081 cgcgtggaac tgggcggcat ggaggcgctg gaggcgttcc tgcgcaagca ttcgctgacg
    19141 ctggattcgc tgcggcggat gctgacgcgg cgcgagcggc gcggcgcgct ggccgcccgc
    19201 gtcgtggcgc gccgggtgaa cctggacgcc gaggcgctgg ccgatttcgc gcgccgccgc
    19261 cgcgaggccg gcctgccgtt ggaggaagtc aacctcgccc agatcctgct ggtctgcccg
    19321 ccggcgctgc gcggcacgcg cgaaggcgag gaagtccaca cgcgcgcgct gcgtctggcc
    19381 cgcgagatcg gcggcgacac gagccgcttc accgaactgg cgatgcagca caccgaggac
    19441 ccggcgggcc gctcgcgcgg cggctacatc ggctggatgg acccgcaggg cctgctgggg
    19501 ccgctgcgcg acgccgtcgc ggcgatggac ccgggcgagg tgtcggcccc tgtcgcgacc
    19561 gacgagggct atcatgtcct gctgctgctg gggcggcgtt ccacccgcga cctgatgttc
    19621 gccgagcagt tcgagaagca gcgcgccgaa ctgctgacgc aactgcgcca gaacgcctcg
    19681 atccagctct tcgacctgga cggcaatccg atcgagacga ttcagtagcg tctgaatcct
    19741 tcccggcggc tttcaaacag aaccagcctt catccctgca gttttcccgc cgaccagagg
    19801 attccgcgct gcaggaccgt cttgaagctg gggatgttcc aactgcggcc gtcgtggccc
    19861 atctgcagac agaagacgcg cgcctgtttg tggcggcggg tccaggccag cgcgcgcatg
    19921 ctcgggttgt aatcggtggt cagcaggata tcgcagccct cgccgggttc ctgcatcgtg
    19981 taggtctcgt cgaacatcgt ccagtcgctc agcccccgca cgatcgggtg atcgggccgt
    20041 tcgatgtgca ccgggaaggt ctgctcgccg taaaagccga atttgcggtc gggcaggccc
    20101 gtcacgtcgg tccagaccgg ctcgtcgggg tatgccagaa tcgcatggtg caggacgacg
    20161 atcccctggg gccggtcgcc caattcggcg atggccttgg ggaccgccgg cgacgcccac
    20221 ggcaattcgg cctccatctt gaggtgcatg ttgtagaaca gcacgacgtc gtattcggcc
    20281 ttctgctcgg gctcggcgaa cgcccagttc tccaccatct gatgcaccgg cttgatgccg
    20341 tccattgcgg cgaacagatc gtggaatcga atcgcgtcgt aggggtgcat gccgacgaga
    20401 acggccacat tgagcggttg agattcaacc atggtcagct tctccggatc ttgaaaagct
    20461 cggccgggcg ccggatgagc gccgcggcgc cattcagccg cgcgggggtc gcgtcttcca
    20521 gcggttgtgc caccagaagt actgttccgg cgcgcggcgg atcgttgatt cgacccaggc
    20581 gttgtagagc gacatgatgc gccgctcctc cgcctcgcgc ggcgccgtcg ggtcggggcg
    20641 gatcaattcc cccatctcca gcagcagccg gccgtcgggc tggcggtaac tggccacggg
    20701 ctggagcggc aggttcagcc ggagcgccag cgaggcgggg ccggtcgacg tggaggcgac
    20761 ctcgccgaac aacgggagga agaccccctc gtggcgggcg tcctgatccg ccaggatgcc
    20821 gatgcagccg ccctcgcgca ggaaccggag cagggggcgc gggacggctt cgcgggtcga
    20881 gaaggtccgc agcgcgttgc gttcgcgcag ccggcgcaac cacgcgtcgg cgtacgggtt
    20941 gtgcatcggc ttgtagatca cgccgcagcg gccgaacgtc agcgcatacc aggcgccggt
    21001 ccactcccag ttgccctgat gcgcgcagaa gaacaggtgg ccgttgccgc gcgcgagggc
    21061 ctggtagcgt tcccaatccg cgggcgagac gattacgcgc cggcggattg cgtcgtgcgg
    21121 cgcgcggctg agccacgcca gttcgagggc gaacagtatt gtgtggacgt gcagctcgcg
    21181 gaggatgcgc cggcgctggg cttcactcat ctcggggaac gcgcgggcca gattgcgctg
    21241 gatgacgccg cgccggatcg gcagcagcgc gtagacgagc gtgcccacac cgcgggcgaa
    21301 cgcccgttgc agcggcgccg gaatgcgccc cagcaacccc agcagaccgc tcagtgcggc
    21361 gaactccagg cggtgcaaca gcgggtgccg ccggcggcgg cggggacgct ccgcggcggg
    21421 gctcatcggt cgtgtcgcgc ggccgccacc atgatgcagg cgtcgtggat caattccaga
    21481 ccgagttcat tggcgcgcgc gatcgccgcc gcatcgcccg cccccggctg catccagatc
    21541 cgcccgacgc cggccgccgc tgcctcatcc aatacctggc gggtcacctc cggcggcgtc
    21601 accatcacgg ccagcccggg tgtttgcggg caggcggcca ggttggggta ggccggctcg
    21661 ccctcgatcg tttcggcgcg cgggttgatt gcatagacgg tccagccgcg gtcgcgcaga
    21721 ttgcgcagca cgatattgcc gtatttggcg cgatcggccg acgcgccgac gaccgccacc
    21781 ggccgcgatt ggatgaattc aaggatcttg gcttccatca ttcccccccg cctctcaaat
    21841 cgtctctcgc ccctcgtcct cgatggccgc tgcggtcgat ggcgccgcac gccaaaacca
    21901 ttctacccta gggatcaagg ccaagccgcc attttagggc agctgcggcg gcaggaaggc
    21961 gtcgtggggg agttcgacgg tctcgccccg cagcagtcgg ccgcggtgct cctccgcttc
    22021 caggccggtc accagggtca aaatcaggtg caggcggcaa ccacgcagca gattcatctg
    22081 ctgggccgac ggcgagagca gaatcagctg ccccccgcgc tggcggagct cgcggtgcag
    22141 cgccatcagg atgcccaggc cggcgctgtc gatgtggtcc agttggctgg tgtcgaagta
    22201 aacacgctcg acggcgggcg ccagcgtctg cagcacgccg ttgcggaatg cgtcgacgac
    22261 gggatgtgtc aatcggcccc gcaggagcca gagctgctcg cggtcgagct gatggacggt
    22321 tgcacgcagc ggctgggaac tcggagaatg gaccatgatg agcacaaatc gcaagcaggc
    22381 cggccagggc gcctgtcgaa ccggacgcgc gactttgcgg ggagcgtgcc gccgaccggg
    22441 cgaagaacgc ggggcccgcc gactattgtt tgagatgcat cagcttttcg agcgattcca
    22501 gatgcttttc cgcgcgcgcc gcgtgcttct cgtccgaacg ctcgcggctg acttccagca
    22561 ccatccgcca gacctcatgg gcgcgcgtat accactccgg gcgcttctca tagacgatgc
    22621 ccaggttgtt cagggccgac aggtttttcg gatcaatatc gagggcctgc tgatacgaga
    22681 tgacggcctt gtcgtaatcg ccctggcgaa tgtattcgac ggcatcgccg cagagctgct
    22741 tgacgcgggc ggcggggtcg tggtccgcgc cgatcagcgg ctcggtcgcc tgcgagccga
    22801 gcgagccggg cgacggattg agcggcgccg agtcgcggtt gacctgcgga tcgatctgcg
    22861 tcaggttgta ggccgactcg ctctcctcct tgctgtcgcg ccagacgaca tactcgtcct
    22921 tctgcaggtt gccgcgaatc agctcggctt cggagccgcc gcggatttca aagatcgcgt
    22981 cgagcttgga gacccggaaa atatcctcca cccgcttttg tggcgagagc agcgccagcc
    23041 gcgtccggtg cttgttggag ctcattttcg tgccgaccag gacgcccagg cccgcgctgt
    23101 cgaggaaact cagttccgcc agatagatat agacctgatc gacctcgggc gtgaacaggc
    23161 tgttcaggcg cgtcttggta tcctggcagt atttcagcac cagccggccg cgcaggatca
    23221 ccagcagctc acggccatgg cggtaggcgt cgatctcgaa ttccttgggc atcggcgaag
    23281 gcccttcttc cgtcctgggg gtatgaacgt caaggcatcg cgggcgcccc agccggcaag
    23341 gccgccgacg ccgcggggct ggtggtcgta tcctcgactt ccgtcaaaat ctgacgcacg
    23401 acatctattg tatcgaacaa tttagtgacc acaagaaaag ttgtctcggc ctgaatccgc
    23461 tcggggcgcg actgcacgat cagccggtgc agctcgacca tcgggcgctg agccagcacg
    23521 ttatacaagt aattgaaggt attccggttg ctgccggtcg aaacgatctt gatcggaatg
    23581 gcgtaaccga tttcgtcatc cttcggctta tacggttcgg catccggatc ggccatgccg
    23641 cccatcatcg agccatccat gccggggaac atgccgggca tccccgggaa catgccgggc
    23701 atgccgggca tcccgcccgg aaccgcgccc ccctcgccct cgcccccggg catcccaggc
    23761 ataccgggca tgccggggaa catccccggg aacatgccgg ggaacatgcc gcccattgcc
    23821 gacatgtcca tttcctcttc ctcgcctggt tccttctggt caacttcatc gtcgatctgg
    23881 gcgagctgga cggcgttcat cggcagcgta tcggcgggcg aacccatccc catcggttgc
    23941 tcgcgcagat aggcgaaccc gcggtgatag accgcgtcga cctgctcgat attgttggcg
    24001 atcgccttct ccagcaggtc gttgatgttg tggatcgtca tgtaggcgaa gtagagcttc
    24061 gtctccttga tccccttcag ttcctcgtcg ggcaggttga tgtcgaggat gtcgagcagc
    24121 atctcgcggt tgagcggggt ctgctggatc atgatcgccc gttcggccgg cagccggtcg
    24181 aggatcagcc cggcgacggc gagccgatag aacagcggga cgaactcgcc gtagcggtcc
    24241 aggccgtcgg cgtgggcgac attgagcccg acctgcgcca gggcggcgtc gtaaatccgc
    24301 cgcgcctgaa agtaggccgt ggagttcggc tgcaggtatt ccagcgttcc cttgcgttcc
    24361 gagagccggc ggacggcgtc ccacagcgcc tcggtctgca ttgcgtcggg cagctcaagc
    24421 ggaatattcc agcccggata cgtccgcgtg ccggggccca ggaagttcat ccgcgtcgtc
    24481 gggttctcgc ggaacgagcg caggtgttcg acttcggcca gcagcaggtc gatctgttcc
    24541 tgcttgcgct gctggatcag ccgctccagc tcttgatttt tcaggttgct gatctcatcg
    24601 ggggtggtgc caggcggagg cggcgggtcg agctgaatcg actcgatcgt cggcatcgtg
    24661 aacgcctcgg gttcctccag ttccagatcg gtgatcccct cgcgcagccg cgccagcatc
    24721 tcgccgacct gttcgttgaa cgtccccagc gtgttctccg ccttctgctg cggtgtcagc
    24781 atcaccaggc tctgcagtgt cgcggccttg gtgtcgtatt cggatttggc ggcggcgacg
    24841 gcgccggctt gcggaccgaa gatcgccaga tgcagaatca ggatgagcac cagggctccg
    24901 accgagccga tgacggcctg ttcgcgcttg cccagcgtca tgattcaact cccgcctttc
    24961 acaagggcgc tccccacggt tcacggggat tgacgcaccc ccatgccgtc ggtagcccgg
    25021 ctttctgtat cataggttct cgttgcggcg gttgccaaga gtttctcccc ccgcaactca
    25081 gatcgtcccc tcgtctctcg tcctcgtgat cgagggacgg aagggacata agggacaaga
    25141 gcgacaaggg acgaaaagga gggcggacag ctttcgagaa ccgaagacga gggacgtgag
    25201 ggaggggcgc ggttacgccg ccgtcgtcca cacctccgcc tgctcgctct catgaatcac
    25261 cgggaacttc aggttttcgt agaccgattt gatccagacc agcaggatga cgcccagcca
    25321 caccagtaag gccatttgat accgatcggg attcgcgccg atctgcccat agacctggcg
    25381 gccgatctcg ctcaggctcg ggaagtgcgt gccgtcggcc agcgaattgg ccagcaggtc
    25441 catgaccccg atctcgacca ggcaccatcc ggccagcacc gccggtccaa ggcagaccca
    25501 ggccacgctc agataatgga gcagccccag cgacagcgag atgtcgaccg ccgcgcagcc
    25561 gctcaggtga tacgcgatca ctgggatcat cagcaggaac aggaccagcg tcagccaggc
    25621 gtaattggtg aagatgaacc aggcggcggc cagaatcagt ccgtccacca gcaggcagac
    25681 atacggcatc cagaaccgcc ggcgtgccag cagggcgtag ccggccggga tgagcggagg
    25741 cgccagcgcg accgccgccg ccagcaggcc gttgcctccc gccgcccgcg ccgaataata
    25801 aatcagcatc gcgttgagca cgatccccgt ccccgccagg atcagcagga actgcgtggg
    25861 cgtcagcgcc tccagttgca gcatgtcccg cagataggcg gccgcctcgg cgcggaactg
    25921 aatgccggcc atcagcgcga gaaacagcgg ggtcgccagc agcaggaacc ggctggccag
    25981 ggccgagcgc cgcttcaatt cgttgggatc gtgcgcggcc cgcgaatcga gcttgtagga
    26041 ggggccgacg tagatatcgt catcggcgcc cgcgccgggc agcgccgggt ccgtgtccag
    26101 ccgtcgtccc agcggggcgc cgcacatcgt gcaggcgagc gcgccattgg cgttgacgga
    26161 atgacattgg gggcattgca tgctgcgttc caacccgcgg gtcagcctgg cggcgccgct
    26221 ttgcgaaatc cggcccggcc cgcgaagccg gcgcgcgccg gtccggccgg gaggagcgga
    26281 tacttctcca gctctcttat cggcgaccgc gccgcgggag tttaggtcgc taaccggagg
    26341 ttcagaatga aggaactggt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26461 accgcgccgc gggagtttag gtcgctaacc ggaggttcag aatgaaggaa ctggttcggc
    26521 tcgatagcgg attttcgaga gccgaggacg agggacgaga gccgagagcc gagagccgaa
    26581 ttttgggtag accttcgccg gtggaggacg aagacaaagc cgaggacgag ggacgtgttt
    26641 caagggcatt ccctcaagac ggcggcgggc cggcatgtta gcatgacccg attcggcgcg
    26701 ccgatgccac ggagagaccc gccgttgccc ttgcttgaag tccaggatct ggaggtagcc
    26761 ttcgcgaccg acgccgggcc gctgcgggcg gtggacggcg tgagcttcgc cgtcgaccgc
    26821 aatcggacgc tggggctggt gggcgagtcg ggctgcggca aatcggtctc ggccctcgcc
    26881 gtcctgcggc tgatcccgaa cccgccgggc cggatcacgg ccggccgcgt gctatggaag
    26941 gagcgcgacc tgctccggct cccggcgcgc caactgccgg cgatccgcgg cgccgagatc
    27001 gccatgatct tccaggatcc gatgacgtcg ctcaatccgg tcttcaccat cgagcagcag
    27061 cttggcgagg tcctgcgcat ccgcttcggg cagggccgcc gccaggcgcg cgcgcgggcg
    27121 cgcgagatgc tcgccaccgt cggcatcgcg gaccccgacc gccgcctgca gaattacccg
    27181 cacgagctct ccggcggcat gaagcagcgg atcatgatcg cgatggccct gctggccgaa
    27241 ccggatctgc tgatcgccga cgagccgacg acggcgctcg acgtgacgat ccaggcgcag
    27301 atcctggcgc tgatccgcga actgcagcag cggaccggga tggcgatggt gctgatcacg
    27361 cacgatctgg gcgtcgtcgc cggcgtctgc gacgaggtcg ccgtgatgta tgccggccgc
    27421 atcgtcgagc acgcgccgtc cgcggcgatc ttcgagaaca gccagcatcc ctacacccgc
    27481 ggcctgctgg ccagcatccc gcgcaagggg cggcgcaagg agtcgcggct ggcgacgatc
    27541 gagggcgtcg tgccgtcgct gctggagccg ccgccgggct gccggttcgc cgaacgctgt
    27601 ccccggcggc gcgagctgcc tgaagcggac cagcgccgct gccggcaggt tgatccggaa
    27661 ctgttgccgg tcgccggcac gccgaacgcc gcggcctgcc attacccctg cagcggcgaa
    27721 agacggtaaa ggggtatccg cgccaccatg caaccgatga ccgagcccgc cgcgccgccg
    27781 ccgcccctgc tcgatgtacg cgacctgcgc aagcactacc cggtgcgcgg cgggctgttt
    27841 gcaacgcaac aggcgacggt caaggcggtc gacggcgtca gcctgcgcgt ccgtccgggg
    27901 gaaaccctgg gcctggtcgg cgaatcgggc tgcggcaaat cgacgctggg gcggacgatc
    27961 ccgcggctgg aggacccgac cgccggcgag gtctactttg ccggccgcaa tctggccgag
    28021 gcccgcggcc gcgaactgtt cgcgctgcgc cgccagatcc agatcatctt tcaggatccc
    28081 tatgcatcgc tcaacccgcg gatgaccgtc ggcgagatcg tgcgcgaacc gctggccgtg
    28141 catcgccgcg gccggcgcgc cgaccagcag cgccgtgtgg cggaactgct cgagctggtg
    28201 ggcctgtcgg ccgacgtgag cgaccgctat ccccacgaat tctcgggcgg ccagcgtcag
    28261 cgcgtcggca tcgcgcgggc gctgacgctc gaaccgaagc tgatcatcgc cgacgagccg
    28321 gtctcggcgc tcgatgtctc ggttcagagt caggtgctca acctgctggt cgagctgcag
    28381 gagcggctcg gcctgaccta tctcttcatc agccacgatc tgtcggtcgt cgaatacctc
    28441 agcgatacga tcgccgtcat gtatctgggc aagatcatgg agatcggccc ggcgcgcgcg
    28501 gtgttcgagg cgccggcgca tccctacacg cgcgcgctga tcgaggcgat ccccgagccc
    28561 gatccgcgcc gccgggtgcg gcacgcgccg gtccgcggcg agacgcccag tccgatcaat
    28621 ccgccgtcgg ggtgcgtctt ccacacgcgc tgtccatatg cggtcgaggc gtgccgcggc
    28681 gtcgttccgc cgctcgaagc ggtcgacccg gcccggcgcg gccaggcgga cgccgacgac
    28741 ctccaccgcg ccgcctgcat caggaaggac gagatttgaa ggggttttcc agagcggaaa
    28801 acgaggacga gagccgagac ccgaattcta gagcaggccg ctgggggcga gcacgagtgc
    28861 ccgtgaacct gcggctcaaa acgaaaaaat gggaggccgt gaacgaccga ttcgaataga
    28921 gaagccttgc cggaatgacc gaccggccag tcccgcaggt gacgccagac ttcagcccgc
    28981 tactttagtg gcgggaaacg gaatgccccg agcgacagag tcctgcgtag ggacggcaaa
    29041 acagcccgaa ttgattggaa cagcgaaggg acgaaaattc gaggcgggcc ttcgggggcg
    29101 atcacgagga cgaagacgag ggacgacttt cagagtgagg ctcagacgtt gaacaggacg
    29161 ttgatcacgt cgccatcgcg cacctcgtat tgtttccctt ccagccgcag cttgcccttc
    29221 ttcttggctt cggcatacga gccgagcgcg agcaactcct cccagccgat cacctccgcg
    29281 cgaatgaagc cgcgctccag gtcgctgtgg atcgcgccgg cggcctcggg ggcgagcgcg
    29341 ccgcggcgca ccgtccaggc gcggcattca tccggcccga cggtgaagaa cgagatctgg
    29401 ccgagcaact cgtagcacag ccggatgacc cggtgggccg ccggctcgcg gatgcccagg
    29461 tcctccatga acgcggcgcg gtcggcctcg tccaggcgcg cgatttccat ttccatctcg
    29521 ccgcacagcc gcgccccgta cgtgtcgggc cacgccgacg ccggccccag gtcgacgggc
    29581 gaatccttgc gcgcggccca gcccgcctcg tcctcgttga tcaggaacat cgtcggcttc
    29641 atcgtcagca gttggaagcc gcgcaggctt ttcgcctgct ccggcgtcag ggcggcggcg
    29701 cgggcgggcc gcccttcctc gagcgcgccc ttgaccgcca cgcacgccgc ctgctcgatc
    29761 tccagcagtt ccttctcgcg tccggccgcg ccgcgcaggc tcttgcccat ccgctccagc
    29821 cggttctcca ctttcttcag gtcgctgagc gccatctcca ggttgatcgc ctccaggtcg
    29881 ccggtcacgt cgggcggctg gccggcggca tcggcgaacc cgcggagcac gaccagcagc
    29941 gcctcggcgg tggcgatctc ggcgatctgc ttgtcgccca ggccgtcgcc ggcgcccccc
    30001 tcgccgcgtg cgacgcccgc cagatcaatg aactccacgg tggcgggggt gtgtttcttg
    30061 gggttgaaca gcgcgctgag ccggtcgagc cggtcgtcgg gcaccttggt gaccgcctgg
    30121 ttggcgccgc cgcgcccgcc gccgaacgcc gacgtctggg cctgctgccc ggtcagggcg
    30181 ttgaagatcg tggttttgcc gctgccttgc aggccgacca ggccgatccg catgatggct
    30241 cctccgcggg cggaatcagc cccttatcct acggacgcgg agggggaatg ccaaagcaaa
    30301 agcggatggg acgatgaacg gcggggaagg cagccgaagc cggggtcctt catgtccctt
    30361 atgtccttta cgtccattcg ccggaggaac cgctcatcac gatcacagag cgcaagtact
    30421 gctggatatc catgtcccat ttggcagaat ggggccggat ggcgaatccg gcccatgacg
    30481 ggcggcgggg cggtccgcat cgtccgcccg ccactctccg cggcgaaggg agcggctgac
    30541 gtgaaagtca cctacaacga acaatacgag cgccatttga aaacctgctt catcggctgc
    30601 ggcgggcatg cctgccgcaa catctacccg acgttccagt acgcgccggt cgacctggcg
    30661 gcggtgtgcg acctggacgc ggcgcgcgcg gcccatatgg cgcgccagtt cggcgcgcgt
    30721 gcgcactaca ccgatcaccg cgagatgctc gaccgggagc gccctgaggt cgtctttatt
    30781 gtcaccaact acgataagga tgcaaaaccg aggtatccgc aactggcgtg cgatgcgatg
    30841 cgcgcgggcg cgcacgtctg gatcgaaaaa ccgccggcgg gctgcctggc cgatgttgag
    30901 atgatgatgg aaaccagccg caagaccggc aaatgggtga tgtgcgggtt caagaagatg
    30961 ttcttcccgg ccaacgtgcg ggcgcatctc ctgtcgcgcc aggaatggtt cggtgcgatc
    31021 cgcacgatca cggcgcgcta tccgcagcat ctgccgggcc ccgaggagcg cgacgatccc
    31081 tggaaactgg tcggtttcct ggaccacatc ggccatccgc attcgctgct gcggctgctg
    31141 ggcggaccgc tcgagtcgat ctatgtggag cgccagcgcg acggggcggc cgtctcggcc
    31201 agagcgggga aggcaacgat caacgtcaca agnnnnnnnn nngacgttga gtgccaacga
    31261 agacgcggtc gcgccgagcg gcgccgtcgg cacgctccac ctctgcccgg ggatcggcgg
    31321 gggctcgccc ctggagcaca ccgagatcgt cggcgagcac ggcaacgtga tcgtcgaaaa
    31381 caacatccgc gtctactgct acagcggcgg cgggccggag ggggggtacg gcgtcgcggg
    31441 cgatttcacc gacacgggcg agcgcggcgg cgcgcagttc tgggagcccg agttctcgct
    31501 cggccagctc tacaacaagg gcctgttcct gctcggctat gcgccggaag tcatctcctt
    31561 ctgcaagcat gccctggccg gcacaccgcc cgagaaggcc gatctggaat gcatctggga
    31621 aatgacccgg atctacgagg cctatctgca gccaaccggg aaggtgatca agatttgagc
    31681 ggaaaggatt gaggcgcgat ttcttgagtg ccgggcgggc ggggggtgac gggatcgacg
    31741 tgcgacatgg atctgggctt tcatgaggcg gtgtcggccc agaaatcctc gaagcggccc
    31801 gactagaggc agcagcgggc ggcggtgatg gcgttgaagc ttgggccgaa gggccgatgc
    31861 cggcgtcact ggctcttgac acggcccggc ggggctggtt agatagcggt tccgggtcgc
    31921 ggcgggcgga tgcgccgacg ccccccgggt gagcgggaat agctcagttg gtagagcgcc
    31981 accttgccaa ggtggatgtc gccggttcga gtccggtttc ccgctccatt
//
LOCUS       DAOPCO010000015        31714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1573_length_31714_cov_4.939670, whole genome shotgun sequence.
ACCESSION   DAOPCO010000015 DAOPCO010000000
VERSION     DAOPCO010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 31714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31714
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_31714_cov_4.939670"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..290)
                     /locus_tag="PLS90_02345"
     CDS             complement(6..290)
                     /locus_tag="PLS90_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01273.1"
                     /translation="MVLLTVGWFLAWSAAGRAHDAGWTTPKGQGPGAGKKPGPDEGVS
                     VNFNKETIELRRDPHGEGLVGQTPLIIRVRTNLPEWRLVVLAPAIDGPAD"
     gene            512..1819
                     /locus_tag="PLS90_02350"
     CDS             512..1819
                     /locus_tag="PLS90_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK01274.1"
                     /translation="MTLASSHADLEVVTRRALDLLARDHTATVSQLADLLAIPSPVGQ
                     EGEAQDWVARRLTELELELHQFELDPAEFAHLPGWLPNDRPHRGRPNVIGIWRGSGGG
                     RSLVLNAHIDTVPADPVERWTSDPWTPRFSGGRLYARGAHDDKAGIVEIIAALRALQR
                     VGFQPRGDIIVQSVIEDEPTGNGTLACAARGFNAEAAWVVDGTPLGEAIVNHCGQIQF
                     FITTYGSGGSIVDKVSGYDAIQLAMEVARELEAFARRAHQQPPAPDWPAVANPLHFAV
                     SRIAGGEWFASRPQKCTLECCMAFNPPETLAGMRERIAAALAAFAQSHPWLRDNPPVL
                     RFEGLATEPVRIYREDHPFFRELGHIYGATAGMPLRWLTLNAWCDLRHFSLHGYVPAV
                     LTGPGNGGGDHGPDEYIETDSMLPVMQALAAFTVRWCGQTGDN"
     gene            1819..2829
                     /locus_tag="PLS90_02355"
     CDS             1819..2829
                     /locus_tag="PLS90_02355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01275.1"
                     /translation="MVVIDAHCHLMDTRVPGFDLIAHKWGGKGWGGGVDDLLAQMDEA
                     GIDHAFLLTSTVIDVMAHFLPEKRDDILRCYDHFVNKACYWRDWELHRDRFTIFADSI
                     DPRVPGYVERAEQDLDRGAKGLKMLPSFTDTTIDDPRWDPIFDLMKDRDVPGIVDLTY
                     WYLRYPWFAPSLHGRFRNFEEFAETVHRRVEKSPGVRLEITHFGTPEIFKTPIATARP
                     GDPVDYDSLQGPIDMIRPHPELICGLANYHMVIPADEPFPYPSGLKIVQILKEELGAD
                     RLTFGTDWPYLEHRSQAELIRSLRAAPFLTPDDVDMILGGTAFNMLKGNLSTADEPVS
                     SS"
     gene            complement(2923..4128)
                     /locus_tag="PLS90_02360"
     CDS             complement(2923..4128)
                     /locus_tag="PLS90_02360"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPK01276.1"
                     /translation="MPSRLRGCLACLRKLAGLISPSGANGRGALRAPSSLPPPPNDYN
                     FIHPPDIICGDQEADPDWRYPLSPEEEQRAMEIYRRSMVIVSHTHCVERSDFDEMQRA
                     GITAAVVKLDIDLFNMINGQCSYGLPGEDWFARGTRAFQRINQMEAEPGSNILIVRDA
                     SDLHRAKREGKVGLILGFEGALALNGELKNLRHFYDLGLRELQPWFLVPNQTMVPKPS
                     GQDLSDFGEALIKEMNRMGVVLDLELMQGQGFARAIRLAKEPVYVSHTAVADLDGKQK
                     HDSPYPFDADQMNDATIRAVAERGGPMGLHFLYPNYIRPRHGTERATLTDLMDHMTYI
                     RDLVGIDHVALGVDFFPATGYTLIEGAGRIAQFPNVARAMVRHGFSDEEISRVLGGNM
                     QRIFEAHLN"
     gene            complement(4361..5230)
                     /locus_tag="PLS90_02365"
     CDS             complement(4361..5230)
                     /locus_tag="PLS90_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK01277.1"
                     /translation="MLKETVMVTLRNDSYVEDFVAQGYVILRGLLDPEVVQLARRAMM
                     QLVDQEARKLIGSGLIDDARVDAPFDRRLYLLYREHLDRAPLSFRKELHLPELYPLFF
                     NPELLDRVEELLGPEIRLYPNYTARPKLPAFAGTEVLWHQDGGYTEQIVGGRDGLADL
                     RMVNVWTPLVPATRENGCMQFIPGTHRLGPVEHVQKTEIYLEIAREFLDPLLDQVVDI
                     ELDPGDVVLFHNLLFHQGQPNRSDHIRWSLDWRYQDATQPTLRVKQGHLARSRQRPES
                     VVRNAEHWGRLEY"
     gene            complement(5382..6962)
                     /locus_tag="PLS90_02370"
     CDS             complement(5382..6962)
                     /locus_tag="PLS90_02370"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPK01278.1"
                     /translation="MSEAGLGVLELGVFDHHGHRVPARFHIEDERGQPWTPAPADRDA
                     WRTAGNQRPVILYSDMRECLGRWTREASCSHLAFGEAGIRLRPGRYTVYVQRGFEFAP
                     QNRVVEVAAGRVTHAEFRLNRVLALPTEGWYGGDIHVHIGRRFAEDNELWTRVLMAED
                     LHAINTMSFKHGQPEPEMDQYAYGEAGEHQHSHYAISPGTEFRDNELYGHITLAGLQE
                     RLDPVSVGPSLGLCENYPLYSMVFEEVRRQGGLGGWAHGGISGPLGGTNNYESLPIEA
                     AMGLVSFVEVVQFRNFLGHATWYGLLNAGLRIAAAGGSDFPFGIWLAPWHPSIGSDRT
                     YARVDGEFTYRKWLDAVGRGQTYASSGPSLRLSVNGAGPGDVVNWEGDRQRVVIRAEA
                     RCVLPLECLEIVVNGALVRRIAASGARLSIEYEEAFDLDRSSWFAARCRGQVAPAVFG
                     GQFPWDLMAHTSPIYLLRGGRPVLVEQDITRLADMVRLIRFSYVRQGKFDVESHREHM
                     LSNCAQALRYYEGLLKMS"
     gene            complement(7128..8309)
                     /locus_tag="PLS90_02375"
     CDS             complement(7128..8309)
                     /locus_tag="PLS90_02375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01279.1"
                     /translation="MRIALFALGRQLQPLAGGELRIHHLAREMALRGHAVDLFSFVLR
                     NHPAGEARPTPNLRIRQQHLLPLDLATLLARARIIPLVSLPLWLRGLRRRIAHWTAAT
                     PYDIVQFEFPWFVEVYGAVRGSGRILYSAHNIESHYWAARGAARPSGRWLRRLERWET
                     RAAGRAAGVIACTEEDRAWLAERGGLEPRRTIVIPNGYDARGIQPPTGEERLAARRVL
                     GLAPEEKLILFIGHDTAPNREAAAFLLDMMQARPSGAPWRLGLVGRVGQAVAPAGRHD
                     VLVPGQVTDVRPWLHAADVSVNPMQSGSGSNLKVVEALAAGLPVVSTRFGLRGYDDLR
                     PFVELAELDSFGAAIGRALTRPRTAPVEVLRRYSWEALAERQLAFYEEITKLSPDGEG
                     R"
     gene            8438..9520
                     /locus_tag="PLS90_02380"
     CDS             8438..9520
                     /locus_tag="PLS90_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01280.1"
                     /translation="MPHLPRGSSRQPVRRAVRALSALAAAALFAAACDKSPQVEQVAG
                     ETPPRPAPTPAAEIIATPPPEAPGLALDLSAISPEAARDSAILPDESAPAAIPDAAEP
                     AGGGELLTLRVPPNMPAAITGFWDGKVENVIYVIHKGWNSAIFDIHLFEDLLLPTTSD
                     VRFNNNRKLDETLFRWKVVEPGREWNFYISQESVLQFDMRGHVFVRGNEAVVEGEITN
                     RSPLEWPHGEAQGLMCIRTRHNPDFWDQPGERILYYRTDGTTLTVARAIQSAERDTFP
                     FFATPLHTNERDLLPRISKVNKTGDRRVTVESNPGSSLGGNRLDTMCCIHANVDLAAK
                     PGETVYFQTVVRFEDLATTSTTTTGP"
     gene            9525..11939
                     /locus_tag="PLS90_02385"
     CDS             9525..11939
                     /locus_tag="PLS90_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK01281.1"
                     /translation="MTRPRLQRRLIHPLAGLLLGLIVACGGEEINGRSLQSWIGRLRY
                     APREAIDETRITAELIHRHQRLRLAKRREAARQLAEAARGAGGEPALADDGLIAAVTW
                     ALGDLDPAVRVPAAASLAWNGRQPEAAALVLLDAMRSNGAPEARRDLLAWARGADLAL
                     DRALPRYAASLQGVLMSDVWAFAGDDAATTTTAPAIESLVAQIQSGPREESLAAALEL
                     ARRGVEAEPALPALRAMIQRGDEPQVRAAAWALGRLGHAAATARPLLAHRLKYARTWE
                     TAVVLAWALARLDPLHIETYDVELDTLRFDDPRARLSAAAAFARMPLDVEPGSRYLAA
                     FLETLRDSDSAVRHAAVVVLGRARLANRASRAALIAAAASDDVQLAAAALLALAKAPD
                     PDPETMGRLLEALGDPRPEIRAAAARGLGELVAALSFPNDALVDAARNGGLRPADPSL
                     PAERLPVDLLLRLRAAEPIRAMLTDPDPALRAAAVEAVIALRDHAPATVESLLRLLSD
                     GDAGVRARATAALGLLGSTRSETAAALRERLNDPHPDVRLCAAAGLIWLNQADFPVLE
                     TMRDGLERASLQLAAEATNLPATVEQIPEYLLTEARLELLQPTPLATYACREALLALA
                     WLGQQGAPLGFSIHEFLRSPAARRLDRRLAAAALLRLHPEIAPPDGIPAPPAVGPRAY
                     REQAVLGLGWLGAAGREAAPDLAAIAADEAAPTPLRVAAAASLSRVVAANHAGPASTI
                     GATTLNPLIAMLRTDDPALRMAGAHALGSIGRAARVVAGDLTLLTEDADPRVRSAARN
                     ALERIR"
     gene            11936..13099
                     /locus_tag="PLS90_02390"
     CDS             11936..13099
                     /locus_tag="PLS90_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01282.1"
                     /translation="MNICFFTHIYPSRAGDYKGVFVHDIAAALVRRGHAVHVVTPRRP
                     GGPARETRDGVHVTRYRFLGWARGTQLAELNGYPPLVLGTLVVGGLWRLWRVARREHC
                     ALIHAFWIVPGGFCAALVGLLTNRPVVATAGGSDLNTFARRRLLAPLVRWTLRRIGHM
                     IAVGSELRRIAVEHGLPADRCSVIPWILQLEATAEPEADRVPLHPGPPGKRLLYVGNL
                     IAPKRVDTIIRALALTLREHPDARLVLVGDGPLREKLADLATAHEVAHAVVFAGAQPH
                     ERIVDYLRQADLFVHCSDNEGLPVAISEALMMGLPVVASHVGSIPDLIREGETGYTVA
                     PDDHAGFARHLCAILDDDALRRELSRQARAFALGYLAPDAVVERIEQVYADLL"
     gene            complement(13117..15558)
                     /locus_tag="PLS90_02395"
     CDS             complement(13117..15558)
                     /locus_tag="PLS90_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01283.1"
                     /translation="MPDHSSKTTLAWIGRATSPAGLFWLTFVLILLLGAWLRAYRLDL
                     PPVDYPADRQRTNMNAVHALIKDTQILDMRRAFLELWILPWLTARTRWVAGLFNLELW
                     TLARLWSFGFGLCTVAGAAAAGFLAADPQWGLERRRRLALLFMTALAINPYHVFISRM
                     ISTESATLALQMAALVCFWMAWRRPRGWRWVGLFVLFFMLAGAAKIPSLIWLPGCFVY
                     FMAQRAYDRRLRIGVFIALLLAVGAVFWVYKLNPFTLYEDYSRRYAWFASQAQGWIGS
                     EPWTRTYLTRLALMLTLPGVLLAAVGLLTAPWLFRITMLTFLVLFYGLVNLNTYNFAH
                     AIIPGMALAAWGVDGLVWMAAASGSSAAGSARLRAALAVGLILLVILVLAPFGPDPAR
                     HPQPRADVLRAAEVIRRLAPPGTIVRHDEPEGSLGYLLRQYDDSLSRELDLMTGYYFS
                     FDRFGEQPVRNAARSWVAWASLPGEPRGLLFSREPERQPDAAEFEGFVAVAGVRSDPP
                     LAVEAETFFVPKELYQAAAHEIRVEPGQQLRIGIRWRNPDHARVAGIRWSSSEWRRYI
                     PVPLRAGGFGIQYGGVLCVPPLDQQTAFYTFELPPSLPVGVYYVNYYPLPEVRWTDVE
                     RGFAAFPFTIRCEGAGESQRTMARDFAELYPMLLDDDPRLWSAHRWFRNMRVIGYETI
                     EEGNYLLSCPRSEPGIYRLTLGGEALPVPDARDPDALWPELSIFLPEDRQRAAATITL
                     ASDRPGRFSAEFVAHAPFDSLLIHVPPARGRQMEKPLWTFDFVPEDFGRQLTRLRSAA
                     LELKRPLFSADPNAP"
     gene            15917..17692
                     /locus_tag="PLS90_02400"
     CDS             15917..17692
                     /locus_tag="PLS90_02400"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPK01284.1"
                     /translation="MTRSFFFAFCLGLSATAFAASDVQWTFGDDTMFNYILNSKSSDI
                     LYDGTLPANDPTLNLIIGKRYGVTIQNPVLHPFQVLAKGTTFNTDVVLLSMGGTTGSF
                     ESDSEVDWTDSGGTVEFTLTPALAAAMAPDANHTPGYRCGMHVDMMRGDFIIYGKGME
                     IADPIPGPIPQGPFVIDLEDVVTGLISPLGVTEPDDGSGRLFVWDQNGTVHIIDNGTL
                     LPTPFLDVSSRLVSLGLGGPGGYDERGLLGFAMHPNFAANPLVYTYTSEPASGSADFE
                     LDLPVGVNPNHDSVIAEWQVDAGNPNLIDLSSRRELLRIEEPQFNHNGGALHFDASGH
                     LFISLGDGGAADDQGNGHGEGNAQNIETILGSILRIDPHGNNSANGQYGIPATNPFVG
                     VPGVDEIYAYGLRNPYSFSIDRVTGDLYVGDAGQNDIEEVTVITAPGANLGWNRKEGS
                     FFFDPNGSGPGFVTTEPAAPIPSGLLDPLVEYDHDEGVVVVGGFVYRSSEFPAFDGTY
                     ICGEFSKNDEGRLFYVDGDDTLREFLIEGGGGVGIFVKGFGQDQAGNVYVTGSNETGT
                     GGTSGVVKKIVPVEVPVGLSSFIME"
     gene            complement(18402..19799)
                     /locus_tag="PLS90_02405"
     CDS             complement(18402..19799)
                     /locus_tag="PLS90_02405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK01285.1"
                     /translation="MGSFWKSHFVTLCLLAIDVIALSILWGESWELIRAFDHRFVRPI
                     NPKDLYLQFLPALLLIWVAVLAFFHQYDHRLRISSLNQVSDILKAGFVLLLVTLAFAN
                     LTKRPTEQLANRVIMPFCVGATAYLYASRSLLRIVKDRMRKAGVGLTRAAIIGAGETG
                     RRVALRIQTHPEIGYELVGFIDRRAAELGATIAGIPVIGDGSRLVDLLLQYRIEEVFL
                     AVPNMDQHAALDLVVECEAANVHFKIVKNDLLQVITDRVKIDEIGDFPVILLREGRLT
                     PAGALLKRAMDLVLTVPLFILSLPLMAVIAICIKLDSRGPVLFVHDRVGQDGRIFKLI
                     KFRTMTTGANPYAVAPSAQSDPRVTRFGRFLRKTSLDELPQLWNVLRGDMSLVGPRPE
                     MPFIVEQYTPWQRRRLDVPQGITGLWQIAGRKQLPLHYNLEYDFYYIRNWSLLLDIVI
                     LIRTIPAVLLGKGAF"
     gene            complement(20324..21001)
                     /locus_tag="PLS90_02410"
     CDS             complement(20324..21001)
                     /locus_tag="PLS90_02410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPK01286.1"
                     /translation="MSEKPMPPDSSAPGKVRISRVYTRTGDAGQTRLVGGQVVPKDHP
                     RLNAYGTVDELQVVIALARDALDATRPLAPAELQPLLELLGEHLCYIQNLLFTVSADL
                     ATRLEDRWDGMVVANQEMVGYAERLIDALNRDLPPLKDFVLPGGHPATSSLHLCRVVC
                     RRAEREVEALARAEPIGEYTRILLNRLSDLFFVMARRVGAGLVEAGLAKPEVIWRRDA
                     PPPPMPE"
     gene            21195..22052
                     /locus_tag="PLS90_02415"
     CDS             21195..22052
                     /locus_tag="PLS90_02415"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPK01287.1"
                     /translation="MPRELPTDDWTETIGRRYRRFVDRMQKGELNAAAEEFARLCHWM
                     QKSSLKFSNRMMAAFTEGWLEVFWQCRRWDLVLKVSEDAESYFGDDPEWRFARGEALF
                     YLGRFDEAREILEKLTHEDFDEPMVFYLLACLAERRHNEQEALRLFQTANKLDSKSML
                     VPVPVNEQEVETLYRECLDELPEKIAWHVKELPIFVDAFPSDELIHSMDPPLDPLVLG
                     VFLGEPMGNETTPWPSDQPRILLFHKNIAKIAGDFDALEDELRRTLFHEVGHYLGFDE
                     DQLEEMGLA"
     gene            complement(22137..23378)
                     /locus_tag="PLS90_02420"
     CDS             complement(22137..23378)
                     /locus_tag="PLS90_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPK01288.1"
                     /translation="MAKAEKAAPVGNMVIGQSGGPTVVINQSLVGAVLGARKHKKIIK
                     NVYGARHGVQGILNQDLINLNDIPRAELLRVGLTPSSALGSVRKKVTPEECRRMFDIL
                     RAHDVHYFFYIGGNDSAETANIVREVAEAENYDLSVFHIPKTIDNDLLGTDHCPGYGS
                     AARFVALAFMGDDLDNRALKGIKINIVMGRHAGFLTAAAALGRSRPDSGPHLVYVPEL
                     PFDEQQFVADVKKVYQKYGRCMIAASEGIADKDGVAIATKFVQEVDSHGNKQLSGTGA
                     LGDYLAQLVKEGFAPEKIRVRADTFGYLQRSFCGCYSEIDSREAYQVGEMAVEAAVSG
                     VKSGTISIIRAKGPGYKVSYKLSPLASVAKNTTQLPRRFLNKDGNNISKAFIEYAMPL
                     VGPLPEVGYLEGARVKPKKIG"
     gene            complement(23617..24930)
                     /locus_tag="PLS90_02425"
     CDS             complement(23617..24930)
                     /locus_tag="PLS90_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK01289.1"
                     /translation="MLKPVRDFLARPLVGSLMKVATGNIAGNGLNLIAMLLLSDSLEA
                     GARGMFTSLQAVMLLIASLSDFGLNTTIIKFYRDLTLAGRAAAAEAIMRAAFWLRLAV
                     AGLIALAGVALAEPLAVHWLDAPDAVGLFRLVCLGALGSSLWMYCQAAMQARQRFGWY
                     AGLTTANHALRLLLFIILIALQRMTVPAAVVVLIAVPFVGSFGASLLWPREYWRARIS
                     RTELREQSAAMFHLSKWIFLSTIICSIIMRLDVILLEWLSTSRQVGLFGNANDIAQGF
                     PLITAAVSTVLLPKLASTRRRAEMRRIVGYFLRSVPVLVVGGGMAMALGHWAIPFIRD
                     GEYSASILVFDLLVVGFSISIIVNPLSFFCLAFNRASWLTWMNLAQLGISLAAGWLLI
                     PPLGAMGAGLSVVLVRLFALGYLVLAYRVLLRMAEDEPTAPPAAG"
     gene            complement(24964..25794)
                     /gene="proC"
                     /locus_tag="PLS90_02430"
     CDS             complement(24964..25794)
                     /gene="proC"
                     /locus_tag="PLS90_02430"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPK01290.1"
                     /translation="MSDLQCLFPTLGFLGAGNMATAIIRATVGRGLLAPETVQILDVV
                     PEKTQQLHQELNVQIAADPAALARSVSALVLAVKPQTMDAALASLRDAVNENHLVISI
                     AAGIPAGRIARGLRNRARVVRVMPNTPALIGCGAAGVAAGPGAGERDVAAVTQLFGAM
                     GIAVPVAEEQLDAVTALSGSGPAYVFRVMEILREAGEEMGLARDISHALTLQTVLGAA
                     RLASESGEDPAVLRQRVTSPGGTTEAALGKFDELNLAGVFKAGVHRARDRSRELAGHD
                     "
     gene            complement(25967..26770)
                     /locus_tag="PLS90_02435"
     CDS             complement(25967..26770)
                     /locus_tag="PLS90_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01291.1"
                     /translation="MDKTITRFRVQAGNLLFEVEGPKDTVQKQLDQHRKQIDRILDEQ
                     IRLIRQGEAATVTAPGIEPERRRPGRPKKVAGAAPAGRGRRPGRAPVIVRDSSLKLGP
                     RHLKRLVEFLRGLAGSSKLSKDGTVFAIAYYLTQNVLNSDRFSAGDVSMAYAQLPRMP
                     FAPAADEVDVVQMLRNLAATSIGKMWVSRNPDGTFAVTQRGKEAGASGQVVRPRGRRP
                     GAVLNKATKKGRRGRPKGSKNVKKKAARRGRPPKQAAKRGPGRPRKNAI"
     gene            27185..28597
                     /locus_tag="PLS90_02440"
     CDS             27185..28597
                     /locus_tag="PLS90_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK01292.1"
                     /translation="MPNRRMQDLSPRSALREAWAPLLLLTVVLLAAYILGILWFRKEV
                     RNAAFELARQQAVQSARRIEQSFPVGPGHLRIIDAGDPMIEEVLYELVHQSPNILAAA
                     LVDPEGTVRQLLLNRSNADVRFVERVWRDLGSDARDEESLTRAIQRTLPHVETEFILL
                     TIDRAPGVKAPIGALRIFLDRHVQHQVVDLAAARMVPWLMFVLALLAAAVAGGLLIIR
                     RQRLGTERLRRERDEAERLSFVGTLAAGLAHEIRNPINALAMQLEMLEEDLPGSTRDS
                     LHPRVERIREGLGGLERTVHEFLAYAAPQQPKPARVELAPVVAALREEARAASPGRRP
                     VRFEFHVPAGLCAWCDEQALRQILANLISNAIQAQAERDETRTTPGATGGEAVVRLTG
                     VRRNTVVELTIEDAGPGIDPAVRSQVFECFYTTRSEGTGLGLPIARRLTELLGGELVL
                     AREASALGGARFLLRLPACD"
     gene            28945..>31714
                     /gene="smc"
                     /locus_tag="PLS90_02445"
     CDS             28945..>31714
                     /gene="smc"
                     /locus_tag="PLS90_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPK01293.1"
                     /translation="MFFKRIEMVGFKSFATRTVCDFIPGTTVVVGPNGCGKSNILDAI
                     KWVVGEQTASQLRGKKMQDVIFSGSASFKPLGVAQVTLTIDNSTRILPSDYEEIQITR
                     RLFRSGESEYLLNKTPCRLRDIHNLFLGTGIGKSSYSMLEQGRVGQIIGAKPIERRFL
                     IEEAAGISKYRARKLEALRKLERTDVDLSRLTDLISEVDRQVTSLKRQANKAERYKGL
                     FDELRRGEQELLVLRSASLRTQLKSLNETLERQRDQLTRLRTAFAEKAAAEEQAREQE
                     EELNERLTDENQALFDLKARLTECDHQITRLNDQIGAHARRKEQIQEEFRELDGRAAE
                     VALRVEEAEQLAADSEQRRAEFSTRYDELNREYQELQASATTRTERLEALSSQVNLLR
                     EEVSRADNEIRMAEALIARQNETREETESLLRALESSFADLAARREELAAQVEALQTQ
                     SESLQQSIEQRRTEQTQRQQRLAALIKELEETHKRLHTGRSRLETLHEMRASYEGYYQ
                     GVREVMLAADAGRLGGILGVAPNLIRARGEHELAIEVALATHLQDIVTRTAEDGKQAI
                     EYLKSAGRGRATFLPLDRLQATPLAANLRQVLGRKGVVGLASELVDFDREIDVAVQFL
                     LGTTIVVEDLDVGLDLGRQGFRGRYVSLDGQLINPAGSMTGGRIQATGLMSREREMRD
                     LQAEVDRLDQRQHQLRGEIERVQIEMSTAHSQLEADQARLDALRLEHAGLRKDLEAAQ
                     RGRDDAERALAERRAQIERIDEEVASRRQAIDQWRARAGEARAQFAAAEAELNQTREE
                     ARGQSGHIMELGVQMAEAKAQVDKAHSQLAEAQRQLQTLERDLESVSRQRQARLAEIQ
                     ELQQQDARLHEQIERIRAELQQTSVEYDEMARQLNADQASREALQLRIKELSAEVERI
                     SRDERELDN"
BASE COUNT         5593 a        11292 c         9722 g         5107 t
ORIGIN      
        1 tcccactagt ccgccggccc atcgatcgcc ggcgccagca ccaccagccg ccattccggc
       61 aaattggtcc gcacccggat gatcagcggc gtctggccga ccagaccctc gccgtgcgga
      121 tcgcgccgca gttcaatcgt ttccttgttg aagttgacac tgaccccttc atcgggtccg
      181 ggcttcttgc ccgcccccgg cccctgtcct tttggggttg tccagcccgc gtcgtgggcc
      241 cggcccgcgg cggaccaggc caggaaccat cccaccgtca gcaggaccac aggacaaatt
      301 cgcgaaaaac aggacatcaa gactccccat cacccatgtc tgaggaaacg cgaaggcttc
      361 ctcaaatcac gcgcgccccc gctgtgcgac ggaggccccc aagaatttac tgggaaatcg
      421 ttattctttg ggatattcta ggtttttcgc ccactcttgc atgtattccc atgccgttcg
      481 ccacgcactt ctccatcact ccaggtgcca gatgaccctc gccagttccc acgccgacct
      541 tgaagtcgtc acccgtcgcg cccttgatct gctcgcccgc gatcataccg ccacggtctc
      601 ccagctcgcc gatctgctgg ccatccccag ccccgtcggt caggagggcg aggcccagga
      661 ttgggtcgcc cgccggctga ccgaactcga gctcgaactc catcaattcg aactggaccc
      721 ggccgagttc gcccatctgc ccggctggct ccccaatgat cgcccccacc gcggccgccc
      781 caacgttatc ggcatctggc gcggttccgg cggcggacgt tcgctggtcc tcaatgccca
      841 catcgacacc gtccccgccg acccggttga gcgctggacc tccgacccct ggaccccgcg
      901 cttcagcggc ggccgcctct acgcccgcgg cgcccacgac gacaaggccg gcatcgtcga
      961 aatcatcgcc gccctgcgcg ccctgcaacg cgtcggattc cagccccgcg gcgatatcat
     1021 cgtccagagt gtgattgagg acgaaccgac cggcaacggc acgctggcct gcgccgcccg
     1081 cggcttcaac gccgaggccg cctgggtcgt cgacggcacc ccgctgggcg aggccatcgt
     1141 caatcactgc ggccagatcc agttcttcat caccacttac ggctcgggcg gctccatcgt
     1201 cgacaaggtc agcggctacg acgccatcca gctcgccatg gaggtcgctc gcgaactgga
     1261 ggccttcgcc cgccgcgcgc accagcaacc gccggccccc gactggcccg ccgtcgccaa
     1321 cccgctccat tttgccgtca gtcgcatcgc gggaggcgaa tggttcgcct cacgccccca
     1381 gaagtgcacc ctcgagtgtt gcatggcgtt caatcccccg gaaacgctcg ccggcatgcg
     1441 cgagcgcatc gccgcggcgc tcgcggcctt cgcccagtcc cacccctggc tgcgcgacaa
     1501 cccgccggtc ctccggttcg aaggtctcgc caccgagccc gtccggatct accgcgaaga
     1561 tcaccccttt ttccgtgagc tgggccatat ctacggggcc accgccggca tgcccctgcg
     1621 ctggctcacg ctcaacgcct ggtgcgacct gcgccatttc agccttcacg gctacgtccc
     1681 ggccgtcctc accggtccgg gcaacggcgg cggcgatcac ggccccgacg aatacatcga
     1741 aaccgactcc atgctccccg tgatgcaggc cctggccgct ttcacggttc gctggtgcgg
     1801 ccagacagga gataactagt ggttgtcatt gatgcgcatt gtcacttgat ggatacccgc
     1861 gtccccgggt tcgatctgat cgcccacaag tggggcggca agggctgggg cggcggcgtc
     1921 gacgacctcc tcgcccagat ggatgaggcc gggattgatc acgccttcct gctgacttcg
     1981 accgtcatcg acgtcatggc gcactttctc cctgagaagc gcgacgacat cctgcgctgc
     2041 tacgatcact ttgtgaacaa ggcctgctac tggcgcgact gggagcttca tcgcgatcgc
     2101 ttcacgatct ttgccgacag catcgatccg cgcgtccccg gctacgtcga acgcgccgag
     2161 caggacctcg atcgcggcgc caaggggctc aagatgctcc cctcgttcac cgacaccacg
     2221 atcgacgatc cgcgctggga ccccatcttc gatctgatga aggatcgcga cgtccccggt
     2281 atcgtcgatc tgacctactg gtatctgcgc tacccctggt tcgcccccag cctgcacggc
     2341 cgtttccgca atttcgagga attcgccgag accgtccacc gccgcgtcga aaagtcaccc
     2401 ggggtccgcc tcgaaatcac ccatttcggc acccccgaaa tcttcaaaac ccccatcgcc
     2461 accgcccgac ccggcgatcc cgtcgactac gattccctcc aggggccgat cgacatgatc
     2521 cgcccccacc ccgaactcat ctgcggcctc gccaactatc acatggtcat tccggccgac
     2581 gaaccgttcc cctaccccag cggcctgaaa atcgtccaga tcctcaagga ggaactcggc
     2641 gccgaccggc tgaccttcgg cactgattgg ccctacctgg agcaccgctc ccaggccgag
     2701 ctgatccggt cgctgcgcgc cgcgcccttc ctcacccccg acgatgtcga catgatcctc
     2761 ggcggcaccg ccttcaacat gctcaagggc aatctgagca ccgccgacga gcctgtctcc
     2821 tcatcctgaa cgcatacggc cgactgtcag tccgctatgg aatgcaaccc attgtatgga
     2881 atccgccggg tccgtcggac ccggcggatt gcgttcgccc gctcagttca ggtgcgcctc
     2941 gaagatccgc tgcatgttcc cgcccagcac ccggctgatc tcctcgtccg agaagccatg
     3001 gcgcaccatc gcgcgcgcca cgttcgggaa ctgcgcgatc cgacccgcgc cctcgatcag
     3061 cgtgtagccc gtcgccggga agaaatcgac ccccagcgcc acgtgatcga ttcccaccag
     3121 atcgcggata tacgtcatat gatccatcag atcggtgagc gtcgcccgct ccgtgccgtg
     3181 ccgcggccgg atatagttgg gatacaggaa atgcagcccc atcggacccc cgcgctcggc
     3241 caccgcgcgg atcgtcgcgt cgttcatctg atcggcgtcg aacggatagg gtgaatcatg
     3301 cttctgtttt ccgtccagat ccgccaccgc cgtgtgcgag acgtagaccg gctccttcgc
     3361 cagccggatc gcccgcgcga agccctgccc ctgcatcagc tccagatcaa ggacgacgcc
     3421 catccggttc atttccttga tcaatgcctc gccgaaatcg cttagatcct gaccgcttgg
     3481 tttggggacc atcgtctgat ttgggaccag gaaccacggc tgcaattccc gcagcccgag
     3541 atcgtaaaag tgccgcaggt ttttcagctc cccgttcagc gccagcgccc cttcgaatcc
     3601 cagaatcagg ccgaccttcc cctcgcgctt ggcgcgatgc aggtccgacg cgtcgcgcac
     3661 gatcaggata ttgctgcccg gctccgcctc catctgattg atccgctgga acgcgcgcgt
     3721 tccccgcgcg aaccagtcct cgcccggcaa cccgtaactg cactggccgt tgatcatgtt
     3781 gaataagtcg atgtcgagct tcaccaccgc cgccgtgatc cccgcccgct gcatttcgtc
     3841 gaagtccgac cgctccacgc agtgcgtgtg cgagacaatg accatcgagc ggcgatagat
     3901 ctccatcgcc cgctgctcct cttcgggcga cagcgggtag cgccagtctg gatcggcttc
     3961 ctggtcgccg cagatgatgt ccgggggatg gatgaaattg tagtcgttgg gcggcggcgg
     4021 cagcgaactc ggcgcgcgca gcgcccctcg gccatttgca cccgacggac tgatcagccc
     4081 cgccagtttc cgcaaacagg cgaggcaccc gcgaagtcgg ctcggcatgc tcgggtctcc
     4141 ttcgtgacgg ctgaatctgg cgccgggcat ctcccccgtt gcaccccggg ggaccgcgtc
     4201 cggtcgcctg cgaaatcgtt tactccgcca ctttagaagg tttcccgttc cctgtcaact
     4261 cactcccgcc ggccggattt cgctcccgct cctgcccgga ttggctttcc ggccgagtgc
     4321 gtccagagcg acccggcagg aggccgatga cctccgccca tcaatactca agccgccccc
     4381 aatgctccgc gttccgcacc acgctctcgg gccgctggcg gctgcgggcc aggtgcccct
     4441 gttttacgcg cagcgtcggc tgcgtcgcat cctggtagcg ccagtccaga ctccagcgga
     4501 tgtgatcgga ccgattgggc tgaccctgat gaaacagcag attgtggaac agcacgacat
     4561 cgcccgggtc cagttcaata tccacgacct gatcgagcag cggatcgagg aactcgcgcg
     4621 cgatctccag gtagatctcc gttttctgca catgctccac gggacccagc cggtgcgtcc
     4681 ccgggatgaa ctgcatgcag ccgttctcgc gcgtcgccgg caccagcggc gtccacacgt
     4741 tcaccatgcg caggtcggcc agcccgtcgc gaccaccgac gatctgctcc gtgtagccgc
     4801 cgtcctgatg ccagagcacc tccgtgccgg caaatgcggg cagtttcggc cgcgccgtgt
     4861 aattgggata gagccggatt tccggaccca gcaactcttc gacccggtcg agcaactccg
     4921 gattaaagaa cagcggataa agttccggca gatggagttc cttgcggaac gacaacggcg
     4981 cccggtccag atgctcccgg taaagcagat acagccggcg atcgaacggc gcgtcgaccc
     5041 gcgcgtcatc gatcaagccg ctgccgatca acttccgggc ttcctgatcg accaattgca
     5101 tcatcgcgcg gcgggcgagc tggacaactt ccgggtcgag cagcccgcgc aggatcacat
     5161 agccttgagc aacaaagtct tcgacgtagg aatcatttct taacgtgacc atcactgtct
     5221 ccttcagcat gggcggccgc gtgagctggg ggccgccgcg ggtgattttc agctttggcg
     5281 ccgggatccc gaccagatca gacgtccgat gttttgcgcc gtagccgtcc ccggttcgag
     5341 aaatctccct gtttgtctcc cctcccctat tcttcgacag cttacgacat cttcagcagc
     5401 ccctcataat agcgcagcgc ctgcgcgcaa ttgctcagca tgtgctcccg atgcgactcc
     5461 acatcgaact tcccctgacg cacgtaactg aaccggatca gccggaccat gtccgccagg
     5521 cgcgtgatgt cctgctccac cagaaccggc cgcccgccgc gcagcaggta gatcgggctg
     5581 gtgtgcgcca tcagatccca cgggaactgc ccgccgaaga cggccggcgc cacctggccc
     5641 cggcaacgcg ccgcgaacca actgctgcgg tccagatcaa acgcctcttc atactcgatc
     5701 gacagccggg cgcccgacgc cgcgatccgc cgcaccaacg caccgttgac gacgatctcc
     5761 agacactcca gcggcaggac gcagcgcgcc tcggcccgga tcaccacccg ctggcgatcc
     5821 ccctcccagt tgaccacgtc gcccgggccg gctccattga cgctcaaccg cagcgacggc
     5881 ccgctgctcg cgtaagtctg cccgcgcccg accgcgtcaa gccatttgcg atacgtgaat
     5941 tccccgtcca cccgcgcata cgtccgatcc gagccgatcg acggatgcca cggcgccagc
     6001 cagatgccga acggaaaatc actccctccg gccgccgcaa tccgcagtcc ggcgttgagc
     6061 agaccgtacc acgtcgcatg ccccaggaaa ttgcggaact ggaccacctc gacaaaactg
     6121 accagcccca tcgccgcttc gatcggcagc gattcataat tgttcgtccc ccccagcggg
     6181 ccgctgatcc cgccgtgcgc ccaaccgccc agtcccccct ggcgccgaac ctcctcgaac
     6241 accatcgagt agagcgggta gttctcgcac agacccagcg acggcccgac gctcaccggg
     6301 tcgagccgct cctgcagccc cgccagcgtg atatggccat agagctcgtt gtcgcgaaac
     6361 tccgtccccg gcgaaatcgc gtaatgcgaa tgctgatgct cccccgcctc gccatacgca
     6421 tattgatcca tctccggttc cggctggccg tgcttgaagc tcattgtgtt gatcgcgtgc
     6481 agatcctccg ccatcagcac gcgcgtccac agctcattgt cctcggcgaa ccggcggccg
     6541 atgtgaacgt ggatatcgcc gccataccac ccctcggtcg gcagcgccag cacgcggttc
     6601 agccggaatt cggcgtgggt gacgcgcccg gccgcgacct ccaccacccg gttttgcggg
     6661 gcgaactcga acccccgctg aacgtagacc gtgtagcgcc cggggcgcag ccggatcccc
     6721 gcctcgccga aggccaggtg cgaacaactg gcctcgcgcg tccagcgccc gaggcattcg
     6781 cgcatgtcgc tgtagaggat caccggccgc tggttgcccg ccgtgcgcca ggcgtcgcgg
     6841 tccgccggcg ccggggtcca gggctggccc cgctcatcct caatgtgaaa ccgcgccggg
     6901 acgcggtggc cgtggtggtc gaagacgccc agctccagca cccccaggcc ggcttcactc
     6961 atcgttgtgc acctcccaca aaggatgccg ccgcgggcgg cgggacatcc ggcggcttga
     7021 tgacggccat taaattgttc cgtccgcgtc ccggattgtc cagtcatgtg atgatctgcc
     7081 gactcacccg gagcggggga ccggcccctg ctctcgcaat ccgtcgctta tcttccctcg
     7141 ccgtcgggcg acagcttcgt gatctcttcg tagaaggcaa gttgcctttc ggccagtgct
     7201 tcccaactgt aacgccgcag cacctcaacc ggcgcggtgc gcggccgggt cagcgcccgc
     7261 ccgatcgccg ccccaaacga atccagctcg gccagctcca cgaacgggcg caggtcatca
     7321 taaccgcgca ggccgaaccg ggtcgatacc acgggcaacc cggcggccag cgcctcgact
     7381 accttcagat tgctccccga acccgactgc atcggattga ccgagacatc ggcggcgtgc
     7441 agccacggcc gcacatccgt cacctggccg gggacaagga catcgtgccg gccggccggc
     7501 gccacggcct gaccgacgcg accgacgagg cccaggcgcc atggggcgcc gctcggccgg
     7561 gcttgcatca tgtcgagcag gaaagccgcc gcttcgcggt tgggggcggt atcgtggccg
     7621 atgaacagga tcagtttctc ctcgggcgca aggcccagca cccggcgcgc ggcgagccgc
     7681 tcctccccgg tcggaggctg gatgccgcgg gcatcgtaac cgttcgggat gacaatcgtc
     7741 cggcgcggct cgaggccgcc ccgctccgcc agccaggcgc ggtcttcctc cgtgcaggcg
     7801 atcacccccg ccgcgcggcc ggcggcgcgc gtctcccagc gctccagccg ccgcagccac
     7861 cgtccggagg ggcgcgccgc tcccctcgcc gcccagtagt gggattcgat gttgtgcgcc
     7921 gaatagagga tgcggccgct tccgcggacg gcgccgtaaa cctcgacgaa ccagggaaac
     7981 tcgaactgga cgatgtcgta aggcgtggcg gcggtccagt gcgcgatgcg ccggcgcagc
     8041 ccccgcagcc agagcggcag gctgaccagc ggaatgatcc gcgctcgagc aagcagcgtg
     8101 gccagatcca gcggcagcaa gtgctgctgg cgaatccgca ggttgggcgt cggccgcgcc
     8161 tcccccgccg gatggttgcg caagacaaac gagaagaggt cgacggcatg accgcgcagg
     8221 gccatttcgc gggcgaggtg gtggatgcgc aactcgccgc ccgccagcgg ctgaagctgg
     8281 cgtcccaggg cgaatagcgc gatcctcaac ggcgaaccgg gcggcatggg gcactatgcg
     8341 cccgcggtga ttgaaaatca atccgccatt ggcataagct ggctgctgcc aacgcgcaat
     8401 cgccccgctg gccccatcgg ccggccgact cgagcccatg ccacacctcc cccgcggttc
     8461 atcacgtcaa ccggtgcggc gcgccgtccg cgcgctgagc gccctggccg cggcggccct
     8521 cttcgcggcc gcttgcgaca agagtccgca ggtggagcag gtcgccggcg aaacgcctcc
     8581 ccgccccgcg ccaacgcccg cggctgagat catcgcgaca cccccgcccg aggcgccggg
     8641 tcttgctctg gatctgagcg ctatttctcc cgaggcggcg cgcgattccg ccattcttcc
     8701 cgatgaatcg gcgccagccg cgataccgga tgccgccgaa cccgccggcg gcggcgaact
     8761 gcttaccttg cgcgttcccc ccaacatgcc cgccgcgatc accggcttct gggacggcaa
     8821 ggtcgagaac gtcatctacg tcatccacaa gggatggaac tccgcgatct tcgacatcca
     8881 cctgttcgag gacctgctgc tgccgaccac cagcgacgtc cgcttcaata ataatcgcaa
     8941 actggatgaa acgttgttcc gctggaaggt cgtcgagccc ggtcgcgaat ggaatttcta
     9001 tatctcccag gagagcgttc tgcagttcga catgcgcggc catgtcttcg tccgtgggaa
     9061 tgaggccgtt gttgaaggcg aaatcaccaa tcggagcccc ctggagtggc cccacggcga
     9121 ggcgcagggc ttgatgtgca tccgcacgcg acataatccc gatttctggg atcagcccgg
     9181 cgagcgaatc ctctattacc ggaccgatgg gacgacgttg accgtcgccc gggcgatcca
     9241 gtccgccgag cgtgatacgt ttccgttctt cgcgacgccg cttcacacca acgagcgcga
     9301 cctgctgccg cgcatcagca aggtcaacaa gacgggcgac cggcgcgtga cggtcgagtc
     9361 gaaccccggc agcagcctgg ggggcaatcg tctcgacaca atgtgctgca tccatgccaa
     9421 tgtggacctg gccgccaaac cgggcgagac ggtatatttc cagaccgtcg tccggtttga
     9481 ggacctcgcc acgacctcca cgacaacgac cggaccctga cgacgtgacg cgcccgcggc
     9541 tccagagacg gctcatccac cccctggccg gtctgctgct gggcctgatc gtcgcctgcg
     9601 gcggcgagga gatcaacgga cggtcgctgc agtcctggat cggccgcctg cgctacgcac
     9661 cgcgcgaggc aatcgacgaa acccggatca ccgccgagct gattcaccgt caccagcggc
     9721 tgcgcctggc caagcgacgc gaggcggccc gtcaactggc cgaagcggct cgcggcgccg
     9781 gcggcgagcc ggccctcgcc gacgatggcc tgatcgcggc cgtcacctgg gcgctgggcg
     9841 acctggatcc ggccgtccgt gtcccggccg ccgccagcct cgcctggaac gggcgacagc
     9901 cggaggccgc cgccctggtc ctgctcgacg ccatgcggtc caacggcgcg ccggaggcgc
     9961 ggcgcgatct gctcgcctgg gcgcgcggcg ccgaccttgc cttggatcgc gccctgcccc
    10021 gctatgccgc ctccctccag ggggtgctga tgagcgatgt ctgggccttc gccggcgacg
    10081 acgcggcgac caccacgacg gcgccggcga tcgagtccct cgtcgctcag atccagtccg
    10141 gcccgcgcga ggagagcctg gcggcggcgc tggaactcgc gcggcgaggc gttgaagccg
    10201 agccggctct ccccgcgttg cgggcgatga tccagcgcgg cgacgagccg caggtgcgcg
    10261 cggcggcctg ggcgctgggc cggttgggcc acgccgccgc gaccgcccgg ccgctgctcg
    10321 cccaccggct caagtatgcc cgcacgtggg agaccgccgt cgtgctggcc tgggcgctcg
    10381 cgcggctcga tccgctacac atcgaaacct acgacgtcga gctcgatact ctccggttcg
    10441 atgacccgcg cgcgcggctc tccgccgcgg cggcgttcgc tcgcatgccg ctcgatgtcg
    10501 aacccggctc gcgatacctc gcggcgttcc tggaaacgtt gcgggattcg gacagcgcgg
    10561 tgcggcacgc cgccgtcgtc gtgctggggc gcgcgcgcct ggcgaaccgc gcgagccgcg
    10621 cggcgttgat cgccgccgcc gcgtccgatg atgtccagct tgccgcggcg gcgctgctgg
    10681 ccctggccaa ggcgcccgac cctgatcccg agaccatggg gcggttgctt gaagcgctgg
    10741 gcgatccgcg cccggagatc cgcgccgcgg ccgcgcgcgg gttgggcgaa ctcgtcgccg
    10801 cactctcctt ccccaacgat gcgctggtcg acgccgcaag gaacggcggc ctgcggccgg
    10861 cggatccgtc gctccccgcc gaacggctcc ccgtggatct gctcctgcgc ctgcgcgcgg
    10921 ccgaaccgat ccgcgcgatg ctgaccgatc ccgaccccgc cctgcgcgcg gccgccgtcg
    10981 aggcggtcat cgcgctgcgc gatcatgcgc cggccacggt tgaatcgctc ctccgcctgc
    11041 tgagcgacgg cgatgcgggg gtgcgcgcgc gggcgaccgc ggcgctcggc ttgctgggga
    11101 gcacgcgcag cgaaaccgcc gcggccttgc gcgagcgcct gaacgacccg catccggacg
    11161 ttcggctgtg cgccgcggcc ggtctgatct ggctcaacca ggcggatttc cccgtcctcg
    11221 agacgatgcg cgacggtctc gagcgggcct cgctgcaact tgcggcggaa gcaacaaacc
    11281 tgcccgcaac tgtggagcag atcccggagt acctgctgac cgaggcgcgg ctggaattgc
    11341 tccaaccaac tcccctggcg acgtatgcct gccgcgaggc cctgctcgcg ctggcctggc
    11401 tcggccagca gggcgcgccg ctcggctttt cgattcatga gttcttgcgc agtccggcgg
    11461 cgcgtcgtct ggatcgccgt ctggcggccg cggctttgct ccgtctgcat cccgaaatcg
    11521 ccccgcccga cggaatcccg gccccgcccg cggtcggtcc ccgcgcttac cgcgagcagg
    11581 ccgtgctggg gctgggctgg ctcggggcgg ccggacgcga agcggcgccg gatctggccg
    11641 caatcgcggc tgacgaagcc gcccccacgc ccctgcgggt cgcggccgcc gcgtcgctca
    11701 gccgcgtcgt ggccgcgaac cacgcggggc cggcgtccac gatcggcgcg acgaccctca
    11761 acccgctgat cgccatgctg cgcaccgacg atcccgcatt gcggatggcc ggcgcccatg
    11821 ccctgggttc gatcgggcgg gccgcgcgcg ttgtggcggg cgacctgacg ctcctgaccg
    11881 aggacgccga tccgcgcgtc cgatccgccg cccgcaacgc cctcgagcgc atccgttgaa
    11941 catctgcttc ttcacccaca tctaccccag ccgggcgggc gattataagg gcgtgttcgt
    12001 ccacgacatc gccgccgcgc tcgtccgccg cggccacgcg gtgcacgtgg tcaccccccg
    12061 gcggcccggc ggcccggcgc gcgagacgcg cgacggtgtg cacgtgacgc gctaccgttt
    12121 cctcggctgg gcccggggga cgcaactcgc cgaactgaac ggctaccccc cgctggtgct
    12181 ggggacgctg gtcgtgggcg gcttgtggcg gctctggcgc gtcgcgcggc gcgaacactg
    12241 cgcgctgatc catgcgttct ggatcgtgcc gggggggttc tgcgcggcgc ttgtcggcct
    12301 gctcacgaat cgccccgtcg tcgccacggc cggcggctcg gatctgaaca cgttcgcccg
    12361 ccggcgcctg ctcgccccgc tggtgcgctg gacgctgcgc cgcatcgggc acatgatcgc
    12421 cgtcggttcg gagctgcgcc gcatcgcggt ggagcacggc ctgcccgcgg acaggtgcag
    12481 cgtcatcccc tggatcctgc aactcgaggc gacggcggaa cccgaagccg accgcgtgcc
    12541 gctccatccc ggaccgccgg gcaagcgcct gctctacgtc ggcaacctga ttgcgcccaa
    12601 gcgcgtcgac acgatcatcc gcgccctggc cctgacgctg cgcgagcatc ccgacgcgcg
    12661 gctggtactg gtgggcgacg gtccgctgcg cgagaaactg gcggacctgg ccacggcgca
    12721 cgaggtcgcg cacgccgtcg tgttcgcggg cgcccagccg cacgaacgca tcgtcgatta
    12781 cctgcgacag gccgatctgt tcgtccattg cagcgacaac gaggggctgc ctgtcgcgat
    12841 cagcgaggcg ctgatgatgg gcctgccggt cgtggcctcg catgtcggca gcatccccga
    12901 tctgatccgc gagggcgaga ccggctacac ggtcgcgccg gacgatcatg cggggttcgc
    12961 gcgccacctg tgcgcgatcc tggacgatga tgcgcttcgc cgggagctct cgcgccaggc
    13021 gcgcgccttc gccctgggtt acctcgctcc cgatgccgtc gtcgaacgga ttgaacaggt
    13081 ctacgccgat ctcctttgac tgcaaagcag cccggctcaa ggagcattcg gatcggcgga
    13141 aaacagaggg cgcttcaatt ccagcgcggc cgaacgcagc cgtgtcagtt gccgcccgaa
    13201 atcctccggc acgaagtcga acgtccacag cggcttctcc atctgcctgc cgcgcgcggg
    13261 cggcacgtgg atcagcagcg aatcaaacgg ggcatgcgca acaaactccg cgctgaaccg
    13321 ccccggccga tccgaggcga gcgtaatcgt cgccgcggcg cgttgccggt cctcgggcag
    13381 gaaaatgctc agttccggcc acaacgcgtc cggatcgcgt gcgtcaggca ccggcagcgc
    13441 ttcgccgccc agcgtcagcc ggtagatccc gggctcgctc cgcggacacg acagcaggta
    13501 gttcccttct tcgatcgtct cgtagccgat cacccgcatg ttgcggaacc agcgatgagc
    13561 cgaccacagg cgcgggtcgt cgtcgagcag catggggtaa agctcggcaa agtcgcgcgc
    13621 catcgtccgt tgagactcgc ccgctccttc acaccggatc gtaaacggga aggcggcaaa
    13681 gccgcgctcg acgtcggtcc agcgtacctc cggcagcgga tagtaattga cgtaatacac
    13741 gccgacgggc aggctcggcg gaagctcaaa cgtatagaac gccgtctgct gatccagggg
    13801 aggcacgcag agcacgccgc cgtattggat tccgaatccc cccgcgcgca gcgggaccgg
    13861 gatgtagcgg cgccactcgc tcgacgacca gcggatcccc gccacgcgcg cgtggtccgg
    13921 attgcgccag cggatgccga tccggagctg ctggccgggc tcgacgcgaa tctcatgcgc
    13981 cgcggcctga tatagctcct ttgggacaaa gaaggtctcc gcctccacgg ccagcggcgg
    14041 atcggagcgc acgccggcga cggcgacaaa gccctcgaac tcagcggcgt cgggctggcg
    14101 ttcgggctcg cgcgagaaga gcagtccgcg cggctcgccc ggcaggctcg cccaggccac
    14161 ccagctccgc gcggcgttgc gcaccggctg ctcgccgaac cggtcaaacg agaaataata
    14221 acccgtcatc agatccagct cgcgcgagag cgaatcgtca tactgccgca gcagatagcc
    14281 caacgaccct tccggttcgt cgtgccggac gatcgtcccc ggcggcgcca gccgccggat
    14341 cacctcggcc gcccgcagca catcggcgcg cggttgcgga tgccgggccg gatcgggccc
    14401 aaagggagca agcacaagga tgacgagcag aatcagcccg acggcgagcg ccgcccgcag
    14461 tctggccgac ccggccgccg acgagcccga cgccgcggcc atccagacca ggccatcgac
    14521 gccccatgcc gccagcgcca tgcccgggat gatcgcatgc gcgaaattat aggtgttcag
    14581 gttgaccagc ccgtagaaca gcacgaggaa cgtcagcatg gtgatgcgaa acagccacgg
    14641 cgccgtcagc aggccgaccg ccgccagcag aacgcccggc agcgtcagca tcagcgccag
    14701 ccgcgtcagg taggtgcggg tccacggctc gctcccgatc catccctgag cctggctggc
    14761 gaaccacgcg tagcgccgcg aataatcctc gtaaagcgtg aaggggttta gtttgtagac
    14821 ccagaagacg gcgcccacgg ccagcagcag ggcgatgaac acgccgatcc ggaggcgccg
    14881 atcgtacgcg cgttgcgcca tgaaatacac aaagcagccg ggcagccaga tcaacgaggg
    14941 gatcttcgcc gcccccgcca gcatgaaaaa caggacgaac agcccgaccc agcgccatcc
    15001 gcgcggccgg cgccaggcca tccagaagca gaccagcgcc gccatctgca gggccagcgt
    15061 cgcgctctcg gtcgagatca tccgcgagat gaatacgtga tatggattga tcgccagcgc
    15121 cgtcatgaac agcaacgcca gccggcgccg ccgttccagc ccccactgcg ggtcggcggc
    15181 caggaacccc gcggccgcgg cgcccgccac cgtgcacaag ccaaacccga acgaccagag
    15241 ccgcgccagc gtccacagct ccaggttgaa caaccccgcg acccagcgcg tcctggccgt
    15301 cagccagggc aggatccaca gttccaggaa cgcccgccgc atgtcgagga tctgcgtgtc
    15361 cttgatcagc gcgtggacgg cgttcatatt cgtccgctgg cgatcggcgg ggtaatccac
    15421 cggcggaaga tccagccgat aggcgcgcaa ccatgccccc agcagcagaa tcaggacgaa
    15481 cgtgagccaa aacagaccgg cgggactcgt cgcccgtccg atccacgcaa gagttgtttt
    15541 gctcgagtga tcgggcatcc ggcggcattc acatcggcgg attgacgggc cgggtcgcgg
    15601 tactcccgcg cgcgctgaat caggcggctc cgccgcggcg cggcgccgct cccaatggca
    15661 cgacacgggc ccatcaaatg gcattccgcg gcgaaacgca agcgcgacat ggcccggcgg
    15721 cgccgcgcca gattgagctt ttcagaaaat ccggccgacc gtcaaaaaaa cttgtacagc
    15781 ggtcccgttt ggcgtgcatt accagatgga gagggatgtc ggggcgtgaa tccggattgt
    15841 ttcccccgat tcagccgggt tcacgccctt ctgcctgttg accgggactc atttttcacg
    15901 gaaaggaccc agatccatga cgcgctcttt cttttttgca ttctgcctcg gcttgagcgc
    15961 cacggcgttt gccgcctccg acgtccaatg gaccttcggc gacgacacca tgttcaatta
    16021 catcctgaac agcaaatctt ccgatatcct ctacgatggc acgctccccg ccaatgatcc
    16081 gacgctcaat ctgatcatcg gcaagcgcta cggcgtcacg atccagaacc cggttctcca
    16141 ccccttccag gtgctggcca aggggacgac gtttaacacc gatgtcgtgc tgctctccat
    16201 gggcggaacc accggatcct ttgagtccga ttccgaggtc gactggaccg acagcggggg
    16261 cacggttgag ttcacgctca cgccggcgct ggccgccgcc atggcgcccg atgccaacca
    16321 tacgcccggc tatcgctgcg gcatgcacgt cgacatgatg cgcggcgact ttatcattta
    16381 cggcaaaggg atggaaatcg ccgaccccat ccccggcccg atcccgcagg gacccttcgt
    16441 catcgacctg gaggatgtcg tcaccggcct catctcgccg ctcggcgtca ccgagccgga
    16501 cgacgggtca ggccgcctgt tcgtctggga ccagaacggc acggttcaca tcattgacaa
    16561 cggcacgctg ctgcccaccc ctttcctgga cgtcagctcg cgtctggtgt cgctcgggct
    16621 cggcggtccg ggcggctatg atgaacgcgg actgctcggc tttgccatgc atcccaactt
    16681 cgccgccaat ccgttggtct acacctacac ctccgagccc gcctccggat cggccgattt
    16741 cgaacttgat ctgcccgtcg gcgtcaatcc caaccacgac tcggtgatcg ccgaatggca
    16801 ggtcgacgcc ggcaatccca acctgatcga cctgagcagc cgccgcgaat tgctgcgcat
    16861 cgaggaaccg cagttcaacc ataacggcgg cgccctccat ttcgatgcca gcgggcactt
    16921 gttcatctcg ctgggcgacg gcggcgcggc cgatgatcag ggcaacggtc acggcgaagg
    16981 caacgcccag aacatcgaaa ccatcctggg gtcgatcctg cggattgatc cgcatggcaa
    17041 caactcggcc aacggccagt acggcatccc cgccaccaac ccgttcgtcg gcgtcccggg
    17101 cgtcgatgag atctatgcct acggtctgcg caatccgtac tccttcagca tcgaccgcgt
    17161 caccggcgat ctctacgtcg gcgacgccgg ccagaacgac atcgaggagg tgacggtgat
    17221 caccgccccg ggcgcgaatc tcggctggaa tcgcaaggaa ggctcgtttt tctttgatcc
    17281 caacggcagc ggccccggct tcgtcacgac cgaaccggcc gcgccgatcc cgtcgggcct
    17341 gctcgacccg ctcgtggaat acgaccatga cgagggcgtt gtcgtcgtcg gcggattcgt
    17401 ctaccgcagc agcgaattcc cggccttcga cggcacgtat atctgcggcg aattcagcaa
    17461 gaacgacgag ggacgcctgt tctatgtcga cggcgacgac accctgcgcg aattcctgat
    17521 cgagggcggc ggcggcgtcg gaatcttcgt caagggcttc ggccaggatc aggcgggcaa
    17581 tgtctatgtg accggctcca acgaaaccgg caccggcggc acgtcgggcg tcgtcaagaa
    17641 aatcgtcccg gtcgaagtcc cggtcggcct ttccagcttc atcatggaat aagcttcatt
    17701 cccatcccca ccccacgcca acagagggag gagccttcat ccccggctcc tccctctgtc
    17761 acgtccgacc aactaaaatc gtccctcgtg ctcgtaatcg cccccaaagg cccacctcag
    17821 aatccggctc ccggctctca aacacacact cccaaaaaga aacctcgctg ccctccaacg
    17881 ggagcgaggt cgagccggct ttcaagcgcg ctggaggagt tacaactgaa cgccccgaca
    17941 ccgcacggga gccggcaact cgtagccgat tccggatttt tcaagggaaa accagttgac
    18001 cccgattccg ggaccgagaa cgaaaacgga ctcctccgcg cttcggcgtt cttgcggtca
    18061 ggacgcgcgg agccagaccc tgccggctaa ctggaactgc tgctgtaaca caactcgggg
    18121 aagacatcgc atatcacatc gcagcctgtc agcgtcagga caagcacaac acagaccagc
    18181 atcgtaacca cgattcgcat ggacgatctc cttcttgccg gagcgggcgt gggcttgcgg
    18241 ccgggcagtc gaagccggct gcgaccaggc gtcaacgaag ccgtgcgcgt cggacgctcg
    18301 gccgggcgga acaggttggc aacgctccac cgcccaaact gttgacagct acatcatcta
    18361 ttcgcggcca gtctagccga cgccttcctc cgctgcaatc ctcaaaatgc ccccttgccg
    18421 agcaaaaccg ccgggatcgt ccgaatcagg atgacaatgt ccagcaacag cgaccagttc
    18481 cgaatgtaat aaaagtcgta ttccaggtta tagtgcagcg gtaattgttt tcgcccggcg
    18541 atctgccata gtccggtgat cccctgaggc acgtcgagcc gccgccgctg ccacggcgtg
    18601 tattgctcca cgatgaacgg catctccggg cgcgggccga ccaggctcat gtcgccccgc
    18661 agcacgttcc acaactgcgg caactcgtcc aggctcgtct tgcgcagaaa ccgcccgaac
    18721 cgcgtcacgc gcgggtcgct ctgcgcgctc ggcgccaccg cgtaggggtt cgcgcccgtc
    18781 gtcatcgtcc ggaacttgat cagcttgaag atccgtccat cctggccaac ccggtcatgg
    18841 acaaagagca ccggaccgcg cgaatcgagc ttgatgcaga tcgcgatcac cgccatcagc
    18901 ggcagcgaca ggatgaacag cggcacggtc aggaccaggt ccatcgcccg tttcagcaag
    18961 gccccggcgg gcgtgagccg cccctcgcgc agcaggatca ccggaaaatc accgatctca
    19021 tcgatcttga cccggtcggt gatcacctgg agcagatcgt tcttgacgat cttgaaatgg
    19081 acgttggccg cctcgcattc caccaccaga tcgagcgccg catgctggtc catgttcggc
    19141 acggccagaa acacttcctc gatgcggtat tgcagcagca ggtcgacgag tcggctgccg
    19201 tcgccgatga cggggatgcc ggcgatcgtc gcgccgagct ccgccgcgcg ccggtcgatg
    19261 aacccgacca gttcatagcc gatctcgggg tgggtctgaa tgcgcagcgc gacgcgccgg
    19321 ccggtctccc cggccccgat gatcgccgcg cgcgtcagcc cgacgcccgc cttgcgcatc
    19381 cggtccttca cgatccgcag cagcgagcgg ctcgcgtata aatacgccgt cgcccccacg
    19441 cagaacggca tgatgacccg gttggcgagc tgttcggtcg gccgcttggt caggttggcg
    19501 aaggccagcg tcaccagcag caatacaaaa cccgccttca ggatatcgct gacctggttg
    19561 agcgagctga tgcgcaggcg atgatcatac tggtggaaga acgccagcac ggcgacccag
    19621 atcagcagca acgccggcag gaactgcagg tagaggtcct tggggttgat cggccggacg
    19681 aagcgatggt cgaacgcgcg aatcagctcc cagctctcgc cccagaggat gctgagcgcg
    19741 atgacgtcga tcgccagcag acacagcgtg acaaaatggc ttttccaaaa ggaacccatg
    19801 cagcggagac agccgatatc cggaagactg cggcaagggg cggcggcccg gcgccccccc
    19861 gggagatcaa cattgcctga ggacgggcca ataggcaacg ggcaatgggg gtccgacccc
    19921 ggccggatcg gccggccccg cccgcgccaa aaggcgaccc atttggcacc gcgcttgcaa
    19981 tgcccccgga cgggatccac aacgcaagac tttcataggg aaggcagaac cactcgattc
    20041 acgaggacga ggacgagaga cgggagacgt aattttgcag gacggacgtc tgatacagcg
    20101 aggatggagc cgtggcgccg cgcgatgagg ataaagagga tcacccgcat caattggaaa
    20161 cgggcataac gctgctggcc gtgttgctga tggtcgccga gaccttcatc gccgtgattt
    20221 tcttctgatt tccggcggcg aagcgtgcag ccgtttgcat ccccgggatg cacggcaatg
    20281 caccctcgcg ccctggcggc gggccgcgag gcgtgtcacg accttactcc ggcatcggcg
    20341 gcggcggcgc gtcgcgccgc cagatcacct cgggcttggc caatcccgcc tcaaccagcc
    20401 ccgccccgac ccggcgcgcc atgacaaaaa acaggtcgga gagccgattc aggagaatcc
    20461 gggtatactc gccgatcggc tcggcacgcg ccagtgcctc cacctcgcgc tccgcgcggc
    20521 ggcagaccac ccggcacaag tgcaggctgg acgtggccgg gtggcccccg ggcaggacga
    20581 aatccttcag cggcggcaga tcgcggttca gcgcgtcgat caaccgttcg gcatagccca
    20641 ccatctcctg gttggccaca accatcccgt cccagcggtc ctccagccgg gtggccaggt
    20701 cggcgctgac ggtgaacaac aggttctgga tgtagcacag atgctcgccg agcagctcca
    20761 gcagcggctg gagctcggcg ggggccagcg gccgggtcgc gtcgagcgcg tcgcgcgcca
    20821 gcgcgatcac cacctgcaac tcgtcgaccg tgccatacgc attcagccgg gggtgatcct
    20881 tcggcaccac ctgccccccc accagccggg tctggccggc atcgccggtg cgggtgtaga
    20941 cgcgcgagat gcggactttg ccgggcgcgg aggagtcggg gggcatgggc ttttcggaca
    21001 tcgcggcgga ccattcagcg gggaattgag caggacgcgc gccgattgta ccagtctctg
    21061 gcgccccccg ccaccccgcg tcggcgggtc acccgcggtc gcccggccgg ccctttcacc
    21121 agattgggga agaaaaagcg cccccgatgg tgtataaatg aagtgacgag aaactccgga
    21181 cgacaggacc gacgatgcct cgcgaattgc cgaccgatga ctggaccgag acgattggaa
    21241 gacgctaccg gcgtttcgtc gaccgcatgc agaagggcga gctcaacgcc gcggccgagg
    21301 agttcgcccg gctgtgccac tggatgcaga aaagcagcct gaagttctcc aatcggatga
    21361 tggccgcgtt caccgagggc tggctggagg tcttctggca gtgccggcgc tgggatctcg
    21421 tcctgaaggt ctccgaggac gccgaaagct acttcggcga tgacccggaa tggcggttcg
    21481 cccgcggcga agccctgttc tacctgggcc ggttcgacga ggcgcgcgaa atcctcgaga
    21541 agctcaccca cgaagacttc gacgagccga tggtcttcta cctgctggcc tgcctcgccg
    21601 aacggcgcca caacgagcag gaggccttgc ggctgttcca gaccgccaac aagctggatt
    21661 cgaagagcat gctcgtgccc gtgccggtca acgaacagga ggtcgaaacg ctctaccgcg
    21721 agtgtctgga cgagctgccc gagaagatcg cctggcacgt caaggaactg ccgattttcg
    21781 tggacgcctt cccctccgac gaactgatcc attccatgga tccgccgctc gacccgctcg
    21841 tgttgggcgt tttcctcggc gaaccgatgg gaaacgaaac caccccctgg ccgtcggatc
    21901 agccccgcat cctgctgttc cataaaaaca tcgccaaaat cgcgggggac ttcgacgcgc
    21961 tggaggacga gctgcgccgg acgctgttcc atgaagtcgg ccactacctg ggcttcgacg
    22021 aggaccagct tgaggagatg ggcctggcct gatccgctaa cgcacgaagc aattgaaatg
    22081 tcccgccggg caggattgcg cggcgggacg ttttttccaa ctggccggtt tcgccgtcag
    22141 ccgattttct tcggcttgac cctggcccct tccagatagc ccacctccgg caacggaccc
    22201 accagcggca tcgcatattc gataaacgcc ttgctgatgt tgttgccatc cttgttcagg
    22261 aaccggcgcg gcagttgcgt cgtgttcttg gcgacgctcg ccagcggact cagcttgtag
    22321 ctgaccttgt agccgggacc cttggcgcgg atgatcgaga tcgtgccgct cttgacgccg
    22381 gagaccgccg cctcgaccgc catctcgccc acctgatacg cctcccgcga gtcgatttcc
    22441 gaatagcagc cgcagaacga acgctgcaga taaccgaacg tgtcggcgcg aacgcggatt
    22501 ttttccgggg caaacccttc cttgaccagt tgggcgaggt aatcacccag ggcgccggtg
    22561 cccgagagct gcttgttgcc gtggctgtcg acttcctgaa caaacttcgt cgcgatcgcc
    22621 acgccgtcct tgtcggcaat cccctcgctg gcggcgatca tgcagcggcc gtatttctgg
    22681 tagaccttct tgacgtccgc cacgaactgc tgttcatcga acggcagctc aggcacatag
    22741 accaggtgtg ggccggaatc gggccggctg cgccccagcg cggccgccgc cgtcaggaac
    22801 ccggcatggc gacccatcac gatgttgatc ttgatcccct tcagcgcgcg gttgtccaga
    22861 tcgtcgccca tgaacgccag cgccacgaac cgcgcggccg agccgtagcc cgggcagtgg
    22921 tcggtcccca gcaggtcgtt gtcgatggtc ttgggaatgt gaaacacgct caggtcatag
    22981 ttttccgctt cggccacttc gcggacgatg ttggccgtct cggccgagtc gttgccgccg
    23041 atgtagaaga agtagtggac gtcgtgcgcg cgcaggatgt cgaacatccg gcggcattcc
    23101 tccggcgtca ctttcttgcg caccgagccg agcgccgagg aaggcgtcag gccgacgcgc
    23161 agcagctcgg cccgcgggat atcgtttaga ttgatcaggt cctgattgag gatgccctgc
    23221 accccgtggc gggcgccgta gacattctta atgatcttct tgtgcttgcg ggcgccgagc
    23281 accgccccga ccaggctctg gttgatgacg accgtcggcc cgcccgactg gccgataacc
    23341 atgttgccca cgggggccgc tttttctgcc ttggccatga ctctgccacc tcgtcgattg
    23401 gtctaaccat ccgtctcgcc ggacgggtcg cgtgcaaccg gaccgcccgc gcgcacagat
    23461 tcctcacact gaaacaagcg cccgatcaca gtcaacgaca atatccgcgc cgaattccgt
    23521 gtgaaggccg caggccgaac cctgcgttac gatccgccag caggatcgaa cccgtggaat
    23581 gggtggccgg agagcaggcc gagcaatccg gccttttcag cctgcggcgg gcggcgccgt
    23641 cggttcgtct tccgccatcc gcaacagcac ccgatacgcc agcaccagat accccagcgc
    23701 gaacagccga accagcacca ccgacaggcc cgcgcccatc gcccccagcg gcggaatcaa
    23761 cagccagccg gccgccaggc tgatcccgag ctgcgccaga ttcatccagg tcagccaact
    23821 ggcccgattg aaggccagac agaaaaacga gagcggattg acaataatgg aaatggagaa
    23881 cccgacgacg agcaggtcga acacgagaat gctcgcgctg tattctccat cgcgaatgaa
    23941 cggaatggcc cagtggccca gcgccatggc catccccccg ccgacgacga ggacgggaac
    24001 agaacggagg aaataaccga cgatgcgccg catctcggcg cggcgccgcg tgctggccag
    24061 ctttggcagc agcacggtcg agaccgccgc cgtaatcagc gggaaaccct gcgcaatgtc
    24121 attggcattg ccgaacagac cgacttgccg gctggtcgaa agccattcga gcagaatgac
    24181 atcgagccgc atgatgatcg agcagatgat cgtggagagg aaaatccatt tggaaagatg
    24241 aaacatcgcc gcggactgct cgcgcagttc cgtccggctg attctggcgc gccagtactc
    24301 ccgcggccac aaaagtgacg ccccgaagga cccgacgaac gggacggcga tcagcaccac
    24361 gaccgccgcc gggaccgtca tccgctgcag tgcgatcagg atgatgaaca acagcagccg
    24421 caacgcgtga ttcgccgtcg tcagcccggc gtaccagccg aaccgctggc gcgcctgcat
    24481 cgccgcctgg caatacatcc atagcgaact gcccagcgcg cccagacaga ccaggcggaa
    24541 cagtccgacc gcatcgggcg catccagcca gtggacggcc agcggttccg ccagcgcgac
    24601 gccggccagc gcgatcagcc ccgccacggc cagccgcagc cagaaggccg cccgcatgat
    24661 cgcttcggcc gccgccgcgc ggccggcgag cgtcaaatcg cggtagaatt taatgatcgt
    24721 cgtgttcagg ccgaaatccg acaggctcgc gatcagcagc atgaccgcct gcagcgacgt
    24781 gaacatgccc cgcgcgcccg cctccagcga gtcgctcagc agcagcatcg cgatcaggtt
    24841 gagcccgttg ccggcaatgt tgccggtcgc caccttcatc aggctgccga ccagcgggcg
    24901 ggcgagaaaa tcccggacgg gtttcagcac cggcgcgcac ctgcgggacg gtgtgaacga
    24961 aacttagtca tggccggcca gttcccgcga gcggtcgcgc gcccgatgca cgccggcctt
    25021 gaacactccg gccagattca gctcgtcaaa cttgccgagc gccgcctcgg tcgttccgcc
    25081 gggggaggtc acccgttggc gcagcaccgc cggatcctcg cccgattcgc tggcaagccg
    25141 ggcggcgccc agcacggtct gcagcgtcag tgcatgggaa atgtcacggg ccaaccccat
    25201 ctcctcgccc gcctcgcgca gaatttccat gacccgaaag acgtaggccg gtccgctccc
    25261 gctcaacgcc gtcaccgcgt ccagttgctc ctcggcgacg ggcacggcga tgcccatcgc
    25321 gccgaacagt tgcgtcaccg cggcaacatc ccgttcgccc gcgcccggac cggccgccac
    25381 gcccgccgcg ccgcagccga tcagcgccgg cgtattgggc atcacccgca cgacgcgcgc
    25441 gcggttgcga agcccgcggg cgatccggcc ggcggggatt ccggcggcaa tcgaaatcac
    25501 aagatggttt tcattaacgg catcgcgcaa cgaggccagc gccgcgtcca tcgtctgcgg
    25561 cttgaccgcc aacacgagcg cgctcaccga tcgcgccagc gcagcgggat ccgccgcgat
    25621 ctgaacgttc aattcctggt gcaactgctg tgtcttttcg ggcacgacgt ccaggatctg
    25681 cactgtctcc ggcgccagca ggccgcgccc gacggtggcg cggatgatgg ccgtcgccat
    25741 attgcccgcc cccaggaacc caagcgtcgg gaataagcat tgcaaatcag acatgtattt
    25801 ccccccaagt aaatcgtccc tcgtcgctcg tcctcgtaat cgcccccaac ggcacacccc
    25861 aaatacgtct ccgcgctccc gcaatccccc ccggctccgc cattaacaat agcacggcgg
    25921 cgcggccgtg tatgcgccgc gccgccctgt tctgtctgcc gccgcgtcag atcgcgttct
    25981 tccgcggacg ccccggcccg cgcttggccg cctgctttgg cgggcgaccc cgccgggccg
    26041 ctttcttctt aacattcttg gaaccttttg gtcgtccacg ccgtcccttt ttcgtcgcct
    26101 tgttcaacac agccccggga cgccggccgc gcggacggac cacctgcccc gaagcgccgg
    26161 cctcttttcc acgctgcgtc accgcaaacg ttccatccgg attgcggctg acccacatct
    26221 tgccgatgct cgtcgccgcc agattgcgca gcatctgcac cacgtccacc tcgtcggccg
    26281 ccggcgcgaa cggcattcgc ggcaactggg cgtaggccat gctgacgtcc cccgccgaga
    26341 accggtcgct gttcagcaca ttctgcgtca gataatacgc aatggcgaaa acggttccgt
    26401 ccttgctgag cttggacgac cccgccaaac cccgcaggaa ttccacaagc cgtttcaaat
    26461 ggcgtggacc caatttcagc gagctatcgc gcacgatgac cggcgcgcgg ccggggcgac
    26521 gtccgcggcc cgccggcgcc gcgcccgcca ctttcttcgg acggcccgga cgccggcgct
    26581 ccggctcgat gcccggagcc gtcacggtgg ccgcttcccc ctggcggatc agccgaatct
    26641 gttcgtcaag gatccgatca atctgcttgc gatgctgatc cagttgcttc tggacggtat
    26701 ccttcggccc ttcgacctcg aacaacaggt tgccggcctg gacccggaag cgggtgatcg
    26761 tcttgtccat gaaatgagcc tctctggtgg attctgcggt ggatcattga ccatttgacc
    26821 gaccgcaagg gactgatctt caaaaaccaa tgaagtactg tcaatgccaa aacgccagcc
    26881 attagagaaa atcgtgccgc tctctccgga ctttgcgttt gaaattgaat atttttcggc
    26941 ctgttgatgc tggcgggtga atgcggtccg cggcgttttt accaaggcca aaatccgtcg
    27001 gatcgacaga atccgaccga atccgacgga ttttgcagcg cggaaacctt gatcagccat
    27061 taattgatcg ggcggccgct gcgtcgggtc gaacacctca atccaagatc gatatccttc
    27121 aggaagtatg ctgaatgcac attgcgcgcg tgctatgttt tcctgggacg aggagtcaat
    27181 gccaatgccg aaccggcgca tgcaggatct ttcaccccgg tcggccctgc gcgaggcgtg
    27241 ggcgcccctg ctgctgctga ccgtggtcct cctcgccgcc tatatcctgg gaatcctgtg
    27301 gttccgcaag gaagtccgca atgccgcgtt cgagctggcg cgccagcagg ccgtgcaaag
    27361 cgcgcggcgc attgaacaat cgtttccggt cgggcccggc catctccgga tcatcgacgc
    27421 cggcgatccg atgatcgagg aagtgctcta cgaactggtt catcaaagcc ccaacatcct
    27481 ggcggcggcg ctggtcgacc ccgaggggac cgtccgccaa ttgctgctca atcgctccaa
    27541 cgccgatgtg cggttcgttg agcgcgtctg gcgggatctg ggatccgacg cccgcgacga
    27601 ggagtcgctt acgcgcgcga tccagcggac cctgccccat gtcgaaaccg agttcattct
    27661 gttgacgatc gaccgcgccc ccggcgtcaa ggcccccatc ggcgcgctcc ggatctttct
    27721 cgatcgccac gtccagcacc aggtggtcga tctggcggcg gcgcgcatgg tcccgtggct
    27781 gatgttcgtc ctcgcgctgc tcgccgccgc cgttgccggc ggtctgctca tcatccgccg
    27841 tcagcggctg gggaccgagc ggctgcgccg cgagcgcgac gaagccgaac gactcagctt
    27901 cgtcggcacg ctcgccgccg gcctcgccca cgaaatccgc aatccgatca acgcgctggc
    27961 gatgcagctt gaaatgctcg aggaagatct gcccggctca acgcgcgaca gtctgcatcc
    28021 gcgggtcgaa cgcatccgcg aggggctggg cgggctggag cgcacggtcc atgagttcct
    28081 ggcctacgcc gcgccgcagc aacccaaacc ggcgcgcgtc gagctggccc cggtcgtcgc
    28141 agcgctgcgc gaggaggccc gcgccgcttc ccccgggcgc cggccggttc gctttgagtt
    28201 ccacgtccca gccggcctgt gcgcctggtg tgacgagcag gccctgcgcc agatcctcgc
    28261 caatttgatc agcaacgcga tccaggcgca ggcggagcgc gacgaaaccc ggaccacgcc
    28321 cggggcgacc ggcggcgagg ccgtcgtccg cctgaccggc gttcgccgca atacggtggt
    28381 cgagttgacg atcgaggatg ccgggccggg gatcgacccg gccgtgcgct cgcaggtctt
    28441 cgaatgcttt tacacgacgc gctccgaagg gaccggcctg ggcctgccga tcgcgcgccg
    28501 gctgaccgaa ctgctcggcg gcgaactggt cctcgcccgc gaggcctcgg cgctgggcgg
    28561 cgcccggttc ctcctgcgcc tgccggcctg cgactgaccg gagtcccgcc cgccccgccg
    28621 ttttccccgc ttgacagata ttgtatcgac ggcaataatc cccgccatat cttgacgctg
    28681 gatcgtcgcg atctctgatt ttcattcact ttccgactgg cctatgctac cttctcctca
    28741 ccttacgagc cgcccgcccg ccgcgacggc ggaacccatc cgttcgaagc tgaaggggga
    28801 tcgctggtcg ccgaacattc cgtgcaatcg atgtcaagcc gtcgttcctg ataccgccgc
    28861 tgatccagtc ccgttgctga acgcgtaacg gggtcgccgc gcaccgtgcg ccccaccgcc
    28921 ccattcccgc aggagatccg gtgcatgttc ttcaaacgca tcgaaatggt tggtttcaag
    28981 tcgttcgcga cccgcacggt ttgtgatttc atccccggca ccaccgtcgt cgtcgggccc
    29041 aacggctgcg gcaaatccaa catcctggat gcgatcaagt gggtcgtcgg cgaacagacc
    29101 gcctcgcagc tgcgcggcaa gaagatgcag gacgtcatct tctccggctc cgcctcgttc
    29161 aagccgctgg gcgtcgcgca ggtgacgctg acgatcgata actcgacgcg gatcctccct
    29221 tcggactacg aggaaattca gatcacgcgc cgcctgttcc gctccggcga gagcgagtat
    29281 ctcctcaaca agaccccctg ccggctgcgc gacattcata acctctttct cgggaccggc
    29341 atcggcaagt cctcctactc gatgctcgaa cagggccgcg tcggccagat catcggcgcc
    29401 aagccaatcg agcgtcggtt cctcatcgag gaagcggcgg gcatctccaa ataccgcgcc
    29461 cgcaaactgg aggcgctgcg caagctggaa cgcaccgacg tcgacctgtc gcgcctgacc
    29521 gacctgatct cggaggtcga tcgccaggtg acctcgctca agcgccaggc caacaaggcc
    29581 gaacgctaca aggggctgtt cgacgagctg cgccgcggcg agcaggaact gctcgtcctg
    29641 cgttcggcca gtctgcgcac gcaactgaaa tcgctcaacg agacgctcga gcgccagcgc
    29701 gaccagttga cgcggctgcg caccgcgttc gccgagaagg cggccgccga ggaacaggcc
    29761 cgcgagcagg aggaggagct caacgagcgg ctgaccgacg aaaaccaggc cctgttcgat
    29821 ctgaaggccc gcctgaccga atgcgatcat cagatcacgc gcctcaacga tcagatcggg
    29881 gcccatgccc ggcgcaagga acagatccag gaggaatttc gcgagctgga cggccgcgcc
    29941 gccgaggtgg cgctgcgcgt cgaggaggcc gagcaactgg cggccgactc cgaacagcgg
    30001 cgcgccgagt tctccacccg ctacgacgag ttgaaccgcg aataccagga actgcaggcc
    30061 tcggccacca cgcgcaccga gcggctcgag gcgctcagca gccaggtgaa cctcctgcgc
    30121 gaggaagtct cgcgcgccga taacgaaatc cgcatggccg aggcgttgat cgcgcggcag
    30181 aacgaaacgc gcgaggaaac cgagtcgctg ctgcgcgcgc tcgaatcctc gttcgccgat
    30241 ctggccgcgc gccgcgagga actggctgcc caggtcgagg cgctgcaaac ccagagcgaa
    30301 tcccttcagc agtcgatcga gcagcgccgc acggaacaga cccaacgcca gcagcggctg
    30361 gccgcgctca tcaaggaact ggaggaaacg cacaagcgcc tgcatacggg ccgctcgcgt
    30421 ctcgaaacgc tccatgaaat gcgcgccagc tacgaaggct actaccaggg cgtgcgcgaa
    30481 gtgatgctgg cggccgacgc gggacgcctt ggcggcatcc tcggcgtcgc ccccaatctg
    30541 atccgcgccc gcggcgagca cgagctggcg atcgaggtcg ccctcgccac ccacctgcag
    30601 gatatcgtga cgcgcacggc cgaggacggc aaacaggcga tcgaatacct caagtcggcc
    30661 ggccgcggcc gcgccacgtt ccttccgctc gatcgactgc aggcaacgcc gctggccgcc
    30721 aacctgcgtc aggtgctcgg gcgcaagggc gtggtcgggc tcgccagcga gctggtcgat
    30781 ttcgaccggg agatcgacgt cgccgtccag ttcctgctcg gcaccacgat cgtcgtcgag
    30841 gacctcgacg tggggcttga tctggggcgc cagggcttcc gcggccgcta tgtctcgctc
    30901 gacggccagc tgatcaatcc cgctggctcg atgaccggcg gccggatcca ggccaccggc
    30961 ctgatgtcgc gcgaacgcga aatgcgcgac ctgcaggccg aagtcgatcg cctcgaccag
    31021 cgccagcacc aactgcgggg cgagatcgag cgcgtgcaga ttgaaatgag caccgcccac
    31081 tcgcaactgg aggccgatca ggcgcggctg gacgccctgc ggttggagca cgccggtctg
    31141 cgcaaggacc tggaggcggc ccagcgcggc cgcgacgacg ccgaacgcgc cctggccgaa
    31201 cgccgcgcgc agatcgagcg gatcgatgag gaagtggcgt cgcgccgcca ggcgatcgac
    31261 cagtggcgtg cgcgcgccgg ggaagcccgc gcccaatttg ccgcggccga ggccgaactg
    31321 aatcagaccc gtgaagaagc ccgcggccag agcggccaca tcatggaact cggcgtccag
    31381 atggccgaag ccaaggcgca ggtcgacaag gctcactcgc agttggccga ggcccagcgc
    31441 cagctccaga cgctcgaacg cgacctggag tcggtatcgc gccagcgcca ggcgcgcctg
    31501 gccgagatcc aggagctcca gcagcaggat gcccgcctgc acgagcagat cgaacgcatt
    31561 cgcgccgaac tgcagcagac gagcgtcgaa tacgacgaga tggcgcgcca gctcaatgcc
    31621 gaccaggcct cgcgcgaagc gctccagctc cggatcaagg agctgagcgc cgaggtcgag
    31681 cgcatcagcc gcgacgaacg cgaactggac aacc
//
LOCUS       DAOPCO010000016        31125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1607_length_31125_cov_5.925845, whole genome shotgun sequence.
ACCESSION   DAOPCO010000016 DAOPCO010000000
VERSION     DAOPCO010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 31125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31125
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_31125_cov_5.925845"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..2862)
                     /locus_tag="PLS90_02450"
     CDS             complement(10..2862)
                     /locus_tag="PLS90_02450"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK01294.1"
                     /translation="MGFPCHRLRAARFIARPALRGVISALLILGAADFAAAEADPSAG
                     ATPGAAAPPLVLNRTTFAPLEGAWWKLADMEDVIEFFVSDVYAAGPRPDQDRDAWVAA
                     MREYRELRRRGELEPGKGRYREVIALDFTGDNRAWTRLADSASRAYALASGDQVTVDV
                     EARWIRGNNNLCVAFDYVNEADGAWAWWSGVLVTLTIPQDGAWHKLSAVVEVPAADLT
                     GRFVRPIVGMDAAHDTTPGYVEIREIDFRLDDPDRMRAIRGQIIDPPVQPIDQETYTH
                     PARDWLKGGFTCHFTFMYDLSFYNPEAGEYELDSFLADGEREFGGYDILLLWHAYPRI
                     GLDPRNQLDFYRDMPGGMAGLRELVDRLHERGVRVFINYNPWDVSTQREGKSDEEMLA
                     ELVAELDVDGIFLDTMTGDSPELRRRVEAVRPGIVLAPESSPTIDNLSICSASWAQWL
                     DDDENPPGLEVRKWLQPRHMRWQTRRTSLSHREEIRRAFFNGSGMLVWENIFGAWNRW
                     SARDRQLWRRAAAILHHFQDNFASDEWDPFYPATEVARDAHQISINRWPGRDATVFTL
                     YRKDDPDLDLMRSGLTLPAGLEELPLFSVADEPEMEYFDLWNGRPARIERGGERVTVY
                     GALDKLTGLGCIAAVRREAVDAAFRALLEKQLNEADRLSVAEDARGGIKSVVLPRPVE
                     PIRPVDASQPPEGMVAVPGGEVHLRITHQRRECGCYPDPDIPASRYKRFTIGTPHNGQ
                     IEHDYTEKVAPFWIDEAAVTNAQFKEFLDQTGYRPRHPENFLKHWPNGEMPPELADHP
                     VVYVDLNDARAYAQWAGKRLPTEAEWHLAAQGTDGRKWPWGNEFDEQNPDPTLVNTTG
                     ATLPARSLPAGRSPYGCYQMSGNVYEWTESERDDGHTRFCIIRGGSYYKATGSDWYMD
                     GGPRPCDHHAKFIMMWPGLDRCATIGFRCVTEMRRR"
     gene            complement(2866..4284)
                     /locus_tag="PLS90_02455"
     CDS             complement(2866..4284)
                     /locus_tag="PLS90_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HPK01295.1"
                     /translation="MKLRIAAAAARVATGRSRGVRLAALAGLALLISAQATAEIVATI
                     QWHEERQVIDGFGTSQAITYAPPLYHWPEPQRSEILDLAFSAEKGIGLTILRNTIPCG
                     DPDDPVPGLQPSPGEWNYTDTEQVWLMHEAVQRGVTTLFGAVWSPPAWMKTSGVTRGG
                     SLKPEHYQDFAEYLARYAKEYAEANQVEIGAISIANEPDNDTTNWASCGWSNREIATF
                     LADYLAPTFAARGVTAQVVAPEIANWERTERHMRATYETPAAIERVDIVAGHLYGGDP
                     TTLFEGAVEHGKKIWMTEASLRFPVFPIDGALNWARTIHEGLVHARLNAWIWWVLANW
                     PDEGLIWLRNEPPAPPRYLVSKAFWALGNYSKFIRPGYVRIEATPNPADELYVSAFKD
                     PRGDRFVIVAVNTGALARPVKFEPVGFTTRLARPYLTSRNSNLEPREDVSFASTVTVP
                     ARSVVTYVGYAASITPETELER"
     gene            complement(4308..6830)
                     /locus_tag="PLS90_02460"
     CDS             complement(4308..6830)
                     /locus_tag="PLS90_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01296.1"
                     /translation="MTARLRSWLCGLLLAACVGAAAHAADDTVYPYFPINASAQGFTW
                     YQKYSDEPGIVEQARRMIEIGADAYKFQVGLDVGWQSWRTTYTDLPDLPRNQKNTIVN
                     LVLNEPAYQTVFDLPFKFYVMWAYAMDGWYFTTNITQAELDREYQQLYDFARYLLTTY
                     QGTGKVFLFGQWETDHVLLEGRDAGDPNQEPTPARLDAVRRWLRNRQKAVEDARNSLP
                     DVTGVEVYNYVEVNAIQVVLDHPEVQRMVTAVLPEITMDLVSYSSYNATNLSLQEPER
                     VANHLNCIMSYGGPTFTGAWPYGPPVFIGEFGLPGGYTTTRAACNKQSLKLCAAWGTP
                     INLFWQVYTSTADNSDSALFQNDGTPNRDHEQLKTFNQRMITLKNARRVWCKRNPTPA
                     ETASLAGAFDTRSNVDLLRAAIDGSSGVTTNAEFLSQLFSDLAMADQIGEAWYNSHLA
                     TLNGGGSRYSTLRSILNSAQFLAAVPTNEYLGYLSAKVLNGPASFPNVSRRSQYEWAL
                     NHADFDADNLQYRLTCDVPEALTDKYSLRLAAYRDEPPRVTFEDLKQDADRFGPAYFI
                     NNGEGVQLTLTSGTELLELRAENAVNGTIVGIEYPAEGTIRWDTVKNRYLQAWIPYNG
                     KGDATSVQFSVDGGPFSGEIWTNRIDGLTDAANNTRPALIDVNALAGLTPGVHELRLR
                     LVCFTTEYSPPEDQGRLEVVLDWLRLGMPPEAETEAGVCQFVGPPEGELAANPPVLEW
                     EAPAEADGLQYIVSLSRDPWFRGPTTFTVHDIEGTSYTPDRPLASGTWYWTVMPINSD
                     GVAGICMARGIGGAGDWRAPYDYEFHSFKIAQSHVPGWGDYR"
     gene            complement(6900..9272)
                     /locus_tag="PLS90_02465"
     CDS             complement(6900..9272)
                     /locus_tag="PLS90_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01297.1"
                     /translation="MWVEDFKQHHLGMDHPWEWPVVILGDGAAAFVSDGENARLSLRG
                     KTSFRASVTTARDFECEAAAAPRLQVRFARLSPEARLKIYGLADADGRVPHLLVNHKN
                     TSSTPSAALLTVDLSEKLPAGTRRLGLRLELDRDNRERVPGEVEVVVDWIKIVAGGAA
                     AAAPGAPRPVAPAQQSAASNPPTLTWEAPEGFTGDRYTVSLSRDPYFSAACTTVLDGV
                     PGRSYQPAAPLAPGRWYWTVAATDASGLSGEFMQDPATSEPLSFTVEGDGDESAEFPY
                     FNLRAGSQAFGSIKKFTDEPKLVEQARRMLEMGSDAIKFLLGADADHANYVSVYKDLS
                     DEIRHRSHTLVELIENEPAYKTVFDMPFKYFVMWVNPMDMTLRLGEGEYNEPEMEQEY
                     QQLYDLTRYFMQTYQGTGKVFLIGHWEGDNMLMEDNDLIKGTPREAKLEDMRKWLANR
                     QKAVEDGRNSLPDVTGVEVYNYAELNIITPVLDDGLPRLINAVLPHVAVDLVSYSAYN
                     CMNHTDELPERAWRHFDYILEHAKFTGAWKHGKPVFMGEYGIPMPLVPSRPARNLIAL
                     KSAASWGSPINLYWSVYTQLENGNSALIELDGTKNQEYHDMRDFLDRMHLLKHATRVW
                     LGRNPTELEANYLALEFDQVKRSELLARIVNSHEYRALVSDEEFLDWALAACGIRDEA
                     AAALRGRLEEASAEGQAPRFDLFLTILDSPEFAQAVSDRDFRNYLAAWLSNGSGQYAG
                     RENVRRSELYLDAIDCEEFSRRDHEFAQLNQVTEAIRAKYQPNFAASGRH"
     gene            complement(9428..11869)
                     /locus_tag="PLS90_02470"
     CDS             complement(9428..11869)
                     /locus_tag="PLS90_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01298.1"
                     /translation="MSVARIFWAWLALQLLLPAPGRAGTVWVEDFKQNHLGMDHEWEM
                     PVVLLGQGSGRFESDGEIARLSLCSGTAFRVQVTTARTIPWDAGQSRLLQMRISRLSP
                     EARIKVYALCDADGREPRLVLNHQNASEALSAQVLTVDLQDKIPAETRSLSLRLELDK
                     SNLKADPRLVEVYIDWIKVGLPGAPDQVPGVPRSASTEADADSGDAEDAQWLQWSAPE
                     GYDDNRYTLSISRDPLFGAACTTILDGIEATRHRLDAELGAGVWYWSVSATSTSGLSG
                     DFLLNEATGKPFSITIPESEEKADAVYPHFNVRAGSQGFGSTIKFTDEPKIVEQARFV
                     LAMGSDSYKFLLGADGEDVNYVTVYQDMSDEIRRKSHTLVELIENEPAYRTVLDLPFK
                     YFAMWANAMDMRVTLGRGKYNAPEMESEYRQLYDLTRFLMQTYQGTGKVFLIGHWEGD
                     NVLMGGNDLINGTPSDAMINDMRRWIANRQRAVEDGRNSLPDVTGVEVYNYAEINILT
                     PVLEHNLPRMINAILSEVPVDLISYSAYNCMNHTDELPERAWQHFDYILEHARFTGAW
                     KHGKPVFVGEYCLPITLNPARPVRNLAAIQSAASWGSPINLFWSAYSVHADGHSSLVE
                     FDGEKNQDYWYLADFIARMHALKNATRTWLGRNPTEIEANYLALEFDRIATSDILARV
                     IDSVEYRVMVEDERFLTEILDGCGIAADAAGQLKQELATRLKQGELTRFEALLEILDS
                     AAFAQAVSEDDFASYLAQVMGEESASGDGKRSERFLEAMNSRSFIQMDLDPDLADRIN
                     AELRAKYRPMFSTWN"
     gene            complement(11967..12971)
                     /locus_tag="PLS90_02475"
     CDS             complement(11967..12971)
                     /locus_tag="PLS90_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK01299.1"
                     /translation="MQLQATSGAPLSPKCERVVRALQFQNPDRLPVLYLNRDQHEGDV
                     VVCWNLAFQDETRTTEWGIKFESLNDGTMGQMTGHPIAEWSDLETYKFPELQPEKRRE
                     VLRQFVAEWPDHYRLASLNLSGFTCYCGLRGFENAMTDFAFDFEHANFLLTKIIDFEM
                     ELIRVAAQENMHGIILWDDWGHQDGLLIAPEMWRQWFRPHYERQFRLAHELGLTVWFH
                     CCGKIDAIVQDFHEIGVDVINISQPNVVDLPRVGAGLRGKQAFMLPISYQTVSISGTP
                     EDIQAEAERLFGLLGTEQGGFVGYVEDYKCMGMSETNYQACKLAFNRLKPDTACATAP
                     "
     gene            complement(13040..14113)
                     /locus_tag="PLS90_02480"
     CDS             complement(13040..14113)
                     /locus_tag="PLS90_02480"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK01300.1"
                     /translation="MSKRNNQSAGKQISTQHVADIVGLARSTVVRALRNDPKLKAETI
                     ERVQLVARQLGYKHNHIARSLALGRTNIVGCVIPNVVNPFFADMITEITTHLASKEFS
                     VLLGVTNDSEEIESKYLQTFMSYRVEGILVAAPPQERAADRYLELKDYERPVVMIGEG
                     EPIGIDSVRSDDESGAFDMMCFLKRCGYSRVAFMGDLEVEGNRKWAGRLDGYRRGLLE
                     NNLSYDPGYLVNCPNIADIGGCLDRLLDRPAPPEVIFAGNDRLAIEVIHVLQKRGLDV
                     PDDLAVVGFDNIRASAEMSPQVTTVDLHIVQTVRIAVGRLLERVDAFHAQTELEDAGR
                     YHVRIKPELRVRETTRMRAPAPV"
     gene            14601..15506
                     /locus_tag="PLS90_02485"
     CDS             14601..15506
                     /locus_tag="PLS90_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPK01301.1"
                     /translation="MKLYSLLNENHVLIGEAVQTMEEALRRMVRAFGAAVAPDQVDPL
                     VHSLLAREEQHPTVIDDRVCIPHMRMESLDVFLLGLLVPEHPIPHPNPEQGPVALIFM
                     ILAPQTKNTMMLQTLAAIARLLKSRETKQAILSVRSAPRLLRWIEDSGIDVKKTLMAG
                     DIMSPITHTVRTDTVLARAVDVLVDAPDEGIPVLNEQGKLVGELTSRELLRLGMPNYL
                     GLIANPSMLDSFEPFENFFQHENSMTVREICGRDIVTVSPSAPVVQVAHLMMTRQKRR
                     IYVLDEGQLRGIIFRKTIIERVLHL"
     gene            15542..16111
                     /locus_tag="PLS90_02490"
     CDS             15542..16111
                     /locus_tag="PLS90_02490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HPK01302.1"
                     /translation="MLAGQLYLASDPELVAERLQARRLLHRFNLSAPDESALREATLR
                     ELFGRIGPSFSIEPPFYCDYGYNILADDGLYMNFGCVVLDVCPVHIGAGVMFGPYVQV
                     YAAHHPLDARLRCAGPELGSPVWIEDRVWVGGGAKICPGVRIGRDSVIGAGSVVTRDI
                     PPGVVAAGNPCRVLRDIPAGDLRSPEELL"
     assembly_gap    16175..16274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16392..18353
                     /gene="mutL"
                     /locus_tag="PLS90_02495"
     CDS             16392..18353
                     /gene="mutL"
                     /locus_tag="PLS90_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPK01303.1"
                     /translation="MSDPPVAAAAPATAPIARLPTRLINQIAAGEVVTRPAAALKELV
                     ENSLDAGAGRIEVSVEEDALSFSVRDDGLGMAADDLVLAVERYATSKIRELDDLQRLT
                     TRGFRGEALAAIAAVSRLEIVTRTPDALEGLRLRVSGGQASPPEPCAANPGTTIAVRD
                     LFFNTPARLKFLRAAVAEWGHMLQAFVRQSLTRPDVAFSIRWRRRPYLDLPAGQSIRD
                     RLAQVLPTGAGQDLLSLDLTLHEVRVTGAVTGPRTTRRDRRHQYAFVNGRPIVFRPLM
                     FALEEAYRGLIMTQRYPMAAVMLTLPGELVDVNVHPTKEQVRFRNEALVAGAVHRAVL
                     EALRAADLVPRLQMPPPGAGLKLAAPPMPKDGTRPATRTAAPAPPLRLDQPDLTDASR
                     DGAQPGDQLDFIPGFGLPGESQGTEGTNPPPSPPPPSNTPAGPAPVPYAEPVARDEAS
                     LLERLRRLPHPPRMLGQVAETYLLAEAGAEGMLVIDQHAAHEKILYLRYLRAAVRPDG
                     IQVQPLMIPHSIDLAPEDEPALETLRPALAEAGFELEPFGGRTWLVQAIPVLFDRLDV
                     PAFLRDLIDDVGQGDLPREMRRLRERICARAACRAAVKAGDRLAPEEMQRLLHDLLGT
                     ADALRCPHGRPTLLLLTRDQLDRQFGRLG"
     gene            complement(18436..18663)
                     /locus_tag="PLS90_02500"
     CDS             complement(18436..18663)
                     /locus_tag="PLS90_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01304.1"
                     /translation="MRTWKQLTLASLMLAAPLMLSSCAYYEDDDDGVDKSVQVERKSS
                     TVHTESDGGTVGSSSERHIERHETTVEEYSD"
     gene            18956..20281
                     /locus_tag="PLS90_02505"
     CDS             18956..20281
                     /locus_tag="PLS90_02505"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK01305.1"
                     /translation="MESTSNEPPAGRPRIVILGGGFGGAYAAMYLERALRPDEAEILL
                     IDRNNYFVFTPLLVEAGVGGLEPRHTVVSLRRFARRSRFRMAEVRGVDLARRIVRIQP
                     PDPPQELEIPYDHLVLAMGSVTLLPPIPGLREFGYEMKSLRDAVALRDRMIRMLEVAD
                     GIDDPAERAARLHLVVVGANFSGVEVAGEFHAFMREASRRYPNVHPRECNVTLVELKE
                     RILQALDPDLARFAARNLMQRSIRVLLRTTVAEVHADHVLLSNGERLAAHTVIWCAGI
                     TSNPTLAGVEGLPRDARGFVLCEPDLRACGLPDVWGIGDCAVNPDPQGNAYPATAQHA
                     VQQARVLARNLRRVLRGQPTEPCRIRNRGSLAALGCRTGVARIGPLKLSGWAAWFMWR
                     TVYLLKMPGLARGVRIALDWTLDLFFRREYSALGLHQRPSPPAPNDSDP"
     gene            complement(20536..21165)
                     /locus_tag="PLS90_02510"
     CDS             complement(20536..21165)
                     /locus_tag="PLS90_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPK01306.1"
                     /translation="MNATSRSSHPAQLYAIIPAAGRSRRMGRPKSLIDVRGQPMLARV
                     VEPLVAAGLEGVLIVTNAALAARLGGLGAAVAVNPDPAAEMIDSVRLGMRWWEAARPI
                     GPQDGFVVCPGDQPGLTARDIAACAAAYRRDPARIVIAGHGGWRGHPLIFPAARRDFV
                     YSPACDDGLNQLARRHAADVVTVDLPNPAVIRNVNTPTDLARLITDEPD"
     gene            21254..22243
                     /locus_tag="PLS90_02515"
     CDS             21254..22243
                     /locus_tag="PLS90_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HPK01307.1"
                     /translation="MSDLLPVLDALVAEHAAGRRCVLCAVVGKKGSTPQPPGAALLVR
                     EDRTTVGTLGGGCVEAEVIRRAHKESLATGEARRMAFVLNHDWGWDDGLICGGRMEIG
                     VMPVAPAADVTPYREAIAAARARREAWFPLVVAEEGRTFAYRIRMEAPPTLLIAGAGH
                     VGQALARLATSLDFHVVVVDDREDYASAARFPAPIERVTRSIPAALADWPIDAHTYVV
                     IVTRGHQNDQRALEAVIHRPAAYIGLIGSERKRRLIFDDLIRQGVAPERLDAVHSPIG
                     LPIHAVTVPEIAVSIAAELIQHRRATPPVLVDGPHVIGAADRPPSGAFAGDAG"
     gene            complement(22259..24382)
                     /locus_tag="PLS90_02520"
     CDS             complement(22259..24382)
                     /locus_tag="PLS90_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01308.1"
                     /translation="MQSPVLFQRILLRRLLLPLIILAGAATAARAGVPEGGFSLAADA
                     LPAGWQAGESGIERTEPAAEPAVLLAGEEDWNDYTAETQVTLGGAEVGSEAGLVLQAE
                     SATDYLVFSLAARKSGPYAILRYQNAELPLTGDQAWLEGLDLTQPHTLRADVHGTAVM
                     GYVDGEPVVSFDFLGTPPADCGTWPLWEDDPDQGQVGLMSVGAAARFSGLTVTPLRDF
                     AHIITPQSGRRDASGMMLPRQSYDKVLRDLTAWAARSQEVIDTSLAPESVRHLPPYIL
                     SMFMRSDDQLWMTLNEFAFNHSLYICGAVTHYLYSGNDDILDEARTLADWHIANRSPA
                     DWALPYMPMSAGTYGPDGTIQTVEWGIEIDKSAYMGLAFLRLYAVTDEPRYLDAALKI
                     AATLRPLQKEDGSWPFRVNAETGEVEVDYACSQLFHSWFFEQLAKVSGDPEDLERSRR
                     AFQWVLENPVKTNKWIGLYGDVPSDSATYDQWIAQETAMMLLDQRDEYPEAVEIAEGI
                     LAWIQEHLMVPCGFHEGIPGLIEQATFIVVLTHHQARLAEMYAKLYEATGKPEYRIKA
                     LETANSTTWCQMSDGKVRQGLGYFAIACPILLSFNDQFCRIMSALPETAPAGENHLLQ
                     TTGLLKSVDYGENRLGYHAIGGGEETLVVQGAPTRVATADNAELPRIEEWFSDDTGWW
                     YDDEQKLMKIRREGPSIQIEFAPAE"
     gene            complement(24443..26593)
                     /locus_tag="PLS90_02525"
     CDS             complement(24443..26593)
                     /locus_tag="PLS90_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01309.1"
                     /translation="MKGLPIAITILLLAAAMRPAAAAWVEPERPLPLTADALAAAEGQ
                     AGGWVARGDGIAHTGAAAEPALLLVGDESWSRYTVETRVRFDEPTTGARAGLVFQARD
                     AARYVYFTLERRHGGFFAVLGFQDAAAIERYRGESPLIEEVFTGDVSRHKDLDAGEWH
                     TLRADVEGAEVVCYLDGEPAARYWFMGTPPPFDHFGQVWPADPTHGRVGVIAAGCAAR
                     FEDLSVSPLKGESPLPTPLRGRRDAQGRLLPLRSYDETMRMLNDWMVTSAEVVDPSPA
                     PASLRHLPPYLLTNWLSTDNRFWLLGGEYGFNHSISILGAVQYYYYSGDERVLQFARD
                     LADWHIENRVPADAKVPHLIQSVVNWQEDGTWEGMEWGLEMDKSAYMGYALLRLYAAS
                     GDERYLNGARDLASVLRKYQSEEGNWPFRINALTGEIKEAYSCSQLWYVHFFDKLYEI
                     DGKEEDRERSRKALQWLLDGPVKDNRWFGVYGDVLIGAESHDQWVPIETAIYLIDHRD
                     EFVGAVRIAEDILDYLKRTLIVDYGFHPDVPGLLEQGSFSVVETIQEFRIAELYAKLY
                     EATGREDYKQAAVAAANSTTWCQVSDGKMRQGLWYLAIDSPLVLSYNYQLCRIMGSIP
                     ETAPRDQNHLLHNTSDLTRIAYRDDGVSYATLGPGEETLVVRARPRQVTAGGEPLNRL
                     RSWGFDEDGWWYDEQTGQLRIRHAAPAIEIVLEQ"
     gene            complement(26868..27986)
                     /locus_tag="PLS90_02530"
     CDS             complement(26868..27986)
                     /locus_tag="PLS90_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK01310.1"
                     /translation="MLNPSAALDHPLANLVGVARGALAVATGDEDRPWQPPVMRRRGG
                     MVMSPLILAAGLAGEAEAAARARIAEALAAFIQSKNMRPRAVQIGEQAVYEIEDVPAT
                     AAAPLAGRVALVTGAAGAIGHGLCRGLLEKGCRVAATDLPGDALTATVESFGREFQDN
                     IIGIPMDVTAPEGVRAAFAALALVWGGVDLVIVNAGIAHVAGLDELQLADFQRLERVN
                     VEGTLNTLAEAGRWLKRQGIGGDVILISTKNVFAPGARFGAYSATKAAAHQLARIAAL
                     ELAEHDIRVNMVAPDAVFSAGEVRSGLWATVGPDRMKARGLDEAGLEEYYRSRNLLKA
                     RVTARHVANAVLFFATRQTPTTGATIPVDGGLPDAAPR"
     gene            complement(28009..28305)
                     /locus_tag="PLS90_02535"
     CDS             complement(28009..28305)
                     /locus_tag="PLS90_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPK01311.1"
                     /translation="MLFVLHATDGPDGAERRNAHREAHVAYIAAQDAAGRIRFAGPLK
                     DEAGKSNGALIIFEAEDPDAARAWVAGDPYVAGGVYSAWEVRAVVQAFPKLSVG"
     gene            complement(28320..29231)
                     /gene="lsrF"
                     /locus_tag="PLS90_02540"
     CDS             complement(28320..29231)
                     /gene="lsrF"
                     /locus_tag="PLS90_02540"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HPK01312.1"
                     /translation="MPEADNKQAKQFHPEIPQPTHGFFAKGAGALDWGMQHRLSRIFR
                     PETGKVVMLAIDHGYFQGPTTGLERVDLNIVPLLEQTDALMLTRGILRSTIPPTYPRG
                     IVMRCSGGPSILKELSNEELAVDVEDAIRMNVAAMTLQVFVGGEHETRTIHNMTRLVD
                     AGLRYGIPVMAVTAVGRELVRDAKYLRLACRICAELGAQMVKTYYCDEDFETVTASCP
                     VPIVMAGGKKLPELDALTMAYNAIQGGAAGVDMGRNIFQSDAPMAMLQAIRKIVHEGY
                     TPKEAMEVYAELKNEQAREKREPVGIG"
     gene            complement(29531..30250)
                     /locus_tag="PLS90_02545"
     CDS             complement(29531..30250)
                     /locus_tag="PLS90_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPK01313.1"
                     /translation="MQVPPVAPFADTTALRRQVIDTCFLMRDRLGYFISTWGNMSVRV
                     EEGLLLTPTRVEYEDLTPEDLCVVSLDGRQVKGTRTPTSETEMHRQILRARPDLGALI
                     HSHSTWASTCACAQRAIPVLIDDMAMVVGGEVRCSRYVPAGRHKELAAAVCEALGDEA
                     WAVLIANHGVIAGGRTLEEAVICCQFVEKAARIFIQAEAIGGVRPIPEELWREERHHY
                     LFKYGKTEDLKDVLKAAPAER"
BASE COUNT         5016 a        11031 c         9568 g         5410 t
ORIGIN      
        1 tcctgccgct cacctccgcc gcatctcggt cacgcagcgg aagccgatcg tcgcacagcg
       61 gtccaggccc ggccacatca tgatgaactt cgcatggtgg tcgcacggcc gcgggccgcc
      121 gtccatgtac cagtcgctgc cggtggcctt gtagtaactg ccgccgcgga tgatgcagaa
      181 gcgcgtgtgc ccgtcgtcgc gttcgctctc ggtccactcg taaacgttgc ccgacatctg
      241 atagcagcca tacgggctgc ggccggcggg aagcgagcgc gcgggcagcg tcgccccggt
      301 cgtgttgacg agcgtgggat cggggttctg ttcgtcgaat tcattgcccc acggccactt
      361 gcgcccgtcg gttccctgcg cggccagatg ccactccgcc tcggtcggca gccgcttgcc
      421 ggcccactgg gcgtaggcgc gggcgtcgtt cagatcgaca tacacgacgg ggtgatcggc
      481 caattccggc ggcatctcgc cattcggcca atgcttgagg aaattctcgg gatgccgcgg
      541 ccggtagccg gtctggtcga gaaattcttt gaactgcgcg ttggtgaccg ccgcttcgtc
      601 aatccagaac ggcgcgacct tctccgtgta gtcatgctcg atctggccgt tgtgcggggt
      661 gccgatcgta aaccgcttgt accggctcgc cgggatgtcc ggatcggggt agcagccgca
      721 ctcgcgccgc tggtgcgtga tgcgcagatg aacctcgccg cccggcaccg ccaccatccc
      781 ttcgggaggc tgactggcgt cgaccggccg gatcggctcg acgggccgcg gcagcacgac
      841 cgacttgatg ccgccgcgcg cgtcctccgc caccgacagc cggtccgctt cgttcaattg
      901 cttttccagc agcgcgcgga acgcggcatc gaccgcctcg cgccgcacgg cggcgatgca
      961 gcccagaccc gtcaacttat caagcgcgcc gtagaccgtg acccgctcgc cgccgcgttc
     1021 gatgcgggcc gggcgcccgt tccacaagtc gaaatactcc atctccggct cgtcggcgac
     1081 gctgaacagg gggagttcct ccaaccccgc ggggagcgtc agccccgacc gcatcagatc
     1141 caggtccggg tcgtccttcc ggtagagcgt gaacaccgtg gcgtcgcggc ccggccagcg
     1201 attgatcgag atctggtgcg cgtcgcgcgc gacctccgtg gcggggtaga acgggtccca
     1261 ctcgtcgctg gcgaagttgt cctggaagtg atgcaggatc gccgccgcgc ggcgccacag
     1321 ttgccggtcg cgcgccgacc agcggttcca cgccccgaag atgttctccc acaccagcat
     1381 cccgctgccg ttgaagaacg cgcggcgaat ctcctcgcgg tggctgaggc tcgtgcggcg
     1441 cgtctgccag cgcatgtggc gcggctggag ccatttgcgc acctcgaggc ccggcggatt
     1501 ttcgtcgtcg tccagccact gcgcccacga cgcgctgcag atggaaagat tgtcgatcgt
     1561 cgggctgctc tcgggggcga gcacaatgcc gggccggacg gcctcgaccc gccggcgcag
     1621 ctcgggcgaa tcgcccgtca tcgtgtccag aaaaatccca tccacatcca gttcggccac
     1681 cagttcggcc agcatctcct cgtcggattt cccttcgcgc tgggtcgaga catcccacgg
     1741 attataatta atgaaaaccc ggacgccccg ctcgtgcagc cgatcgacaa gctcgcgcag
     1801 cccggccatc cctccgggca tgtcgcggta gaagtcgagt tgattgcgcg gatccaggcc
     1861 gatccgcgga tacgcgtgcc agagcagcag gatgtcgtag ccgccgaatt cccgctcgcc
     1921 gtcggccagg aacgaatcga gttcgtactc gcccgcctcg gggttataga agctcaggtc
     1981 gtacatgaat gtgaaatggc aggtgaagcc ccccttgagc cagtcgcgcg ccggatgggt
     2041 gtaggtttcc tgatcgatcg gctgaaccgg cgggtcgatg atctggccgc ggatcgcgcg
     2101 catccggtcc ggatcgtcga gccggaaatc aatctcccgg atctcgacat acccgggcgt
     2161 cgtgtcgtgc gcggcgtcca tgccgacgat cgggcgcacg aaccggccgg tcaggtcggc
     2221 ggcgggaacc tccaccacgg cgctcagttt gtgccatgcg ccgtcctgcg ggatggtcag
     2281 cgtgaccagc acgccgctcc accaggccca cgcgccgtcg gcctcgttca cgtaatcgaa
     2341 cgcgacgcac aggttgttgt tgccccggat ccagcgcgct tcgacgtcga ccgtcacctg
     2401 gtcgccggag gcgagggcgt aggcgcgcga cgcactgtcg gcgaggcggg tccaggcgcg
     2461 gttgtcgccg gtgaagtcca gcgcgatcac ctcgcgatag cgccccttgc cgggctccag
     2521 ttcaccccgc cggcgcagtt cgcggtactc gcgcatcgcg gcgacccatg cgtcgcggtc
     2581 ctgatcgggg cggggaccgg cggcgtagac gtcggagacg aagaactcga tcacgtcctc
     2641 catatccgcc agtttccacc aggcgccctc cagcggcgcg aacgtggtcc ggttgagcac
     2701 cagcggcggg gccgcggcgc cgggcgtcgc gcccgcggac ggatcggcct ccgcggcggc
     2761 gaaatccgcc gcgcccagga tcaacagcgc gctgatgacc ccgcgcaatg ccgggcgggc
     2821 gatgaaccgt gcggcgcgaa gacgatgaca ggggaatccc atgggctatc tttccagttc
     2881 ggtttcaggc gtgatcgacg cggcataccc cacgtaagtg acaacggagc gggccggcac
     2941 ggtgacggtc gaggcaaacg agacgtcttc ccgcggctcg agattagaat tgcgcgacgt
     3001 cagatagggc cgcgccagcc gcgtggtaaa accgacgggt tcaaatttca ccgggcgcgc
     3061 cagcgcgccg gtattgaccg cgacgatcac aaaccggtcg ccgcgcgggt ccttgaacgc
     3121 cgagacatac agctcgtcgg ccgggttggg cgtcgcctcg atccgcacat accccgggcg
     3181 aatgaacttg ctgtagttgc ccagcgccca gaacgccttg ctaaccagat accgcggcgg
     3241 cgccgggggt tcgttccgca gccagatcag gccctcgtcg ggccagttcg ccagcaccca
     3301 ccagatccag gcgttgagcc gggcatgcac gagtccctcg tggatcgtcc gcgcccagtt
     3361 cagcgcgcca tcgatcggga acacgggaaa gcgcagcgag gcctcggtca tccagatttt
     3421 cttgccgtgc tccaccgccc cttcgaacag cgtcgtcggg tcgccgccgt aaaggtgacc
     3481 ggcgacgatg tccacgcgct cgatggcggc cggcgtttca tacgtcgccc gcatgtggcg
     3541 ctcggtccgc tcccagttgg cgatctccgg cgccacgacc tgcgccgtga cgccgcgcgc
     3601 cgcgaacgtc ggcgcgagat agtccgccag gaacgtcgcg atttcccggt tcgaccagcc
     3661 gcacgaggcc cagttcgtcg tgtcgttgtc gggctcgttg gcgatcgaga tcgcgccgat
     3721 ctcaacctga ttggcctcgg cgtattcctt cgcgtaacgg gcgagatact ccgcgaaatc
     3781 ctgatagtgt tccggcttga gcgacccgcc gcgcgtcacg ccgctcgttt tcatccaggc
     3841 cggcgggctc cacacggcgc cgaacagcgt cgtcacgccc cgctgcaccg cctcgtgcat
     3901 cagccacacc tgctcggtgt cggtgtaatt ccactccccc gggctcggct gcaggccggg
     3961 caccggatcg tcggggtcgc cgcacggaat ggtgttgcgc aggatcgtca gaccgatccc
     4021 cttctccgcg ctgaaggcca ggtcgaggat ttcgctccgt tgcggctcag gccagtgata
     4081 cagcggcggc gcgtaggtga tggcctggct ggttccaaac ccatcgatca cctgccgctc
     4141 ctcgtgccac tggatcgtgg cgacgatctc ggcggtggcc tgcgcggaaa tcagcagggc
     4201 caggcccgcc agcgccgcaa gccgcacgcc gcgcgatctt cccgtcgcga cccgggcggc
     4261 cgccgcggcg atcctcagtt tcaacttcaa ggcattccct cccatgctca ccggtaatcc
     4321 ccccagccag gcacatggct ttgcgcgatt ttgaacgaat gaaattcata gtcatagggg
     4381 gcgcgccaat cccccgcgcc gccgatcccc cgcgccatgc agatgcccgc gacgccgtcg
     4441 gaattgatcg gcatcacggt ccaataccag gtccccgacg cgagcgggcg atccggcgta
     4501 tagctggtcc cttcgatgtc gtgcaccgtg aacgtcgtcg ggccgcggaa ccacggatcc
     4561 cgcgagaggc tcacaatgta ctgcaggccg tccgcttcgg ccggcgcctc ccactcgagc
     4621 accggcggat tggcggccag ctccccctcc ggcggaccga caaactgaca gacgcccgcc
     4681 tcggtttccg cctcgggcgg catccccagc cgcaaccagt ccaacaccac ctccagccgg
     4741 ccctggtcct cgggcgggct gtattccgtc gtgaagcaga cgagccgcag acgcagctca
     4801 tggacgcccg gcgtcagacc cgccagcgca ttgacgtcga tcagcgccgg acgggtgttg
     4861 ttggccgcgt cggtcaggcc gtcgatccgg ttcgtccaga tctcgccgct gaacgggccg
     4921 ccgtccacgc tgaactgcac cgacgtggca tcccccttgc cgttgtaggg aatccatgcc
     4981 tgcaaatagc ggttcttcac cgtgtcccag cggatcgtcc cctcggctgg atactcgatg
     5041 ccgacgatcg tcccgttgac ggcgttttcg gctcgcagtt cgagcagttc ggttcccgaa
     5101 gtaagcgtga gctgcacgcc ctctccgttg ttgatgaaat aggccggacc gaacctgtcg
     5161 gcgtcctgct tcagatcctc gaacgtcacg cgcggcggtt cgtcgcgata ggcggccagc
     5221 cgcagggaat acttgtcggt caacgcctcc ggcacgtcgc acgtgagccg gtactgcaga
     5281 ttgtccgcgt cgaagtcggc gtggttgagc gcccactcat actgcgaccg gcgcgaaaca
     5341 ttcgggaacg acgcgggccc gttcagcacc ttcgcgctca gatagccgag gtattcattc
     5401 gtcggcaccg ccgccaggaa ttgcgcggaa ttgaggatcg agcggagggt cgagtagcgc
     5461 gaaccgccgc cgttcagggt cgccagatgg ctgttgtacc acgcctcgcc gatctgatcc
     5521 gccatggcca ggtcgctgaa gagctgcgac agaaattcgg cgttggtcgt gaccccgctc
     5581 gatccgtcga tcgccgcgcg caggagatcg acgttggacc gcgtgtcgaa cgccccggcg
     5641 agcgaggcgg tctccgccgg cgtcgggttg cgcttgcacc agacccgccg cgcgttcttc
     5701 agcgtgatca tccgctggtt gaatgtcttc agctgctcgt gatcgcggtt gggcgtgccg
     5761 tcgttctgga acagcgcgga atcgctgttg tccgccgtcg acgtgtagac ctgccagaac
     5821 agattgatcg gcgtgcccca ggcggcgcac agcttgagcg attgcttgtt gcacgccgcc
     5881 cgcgtggtgg tatagccgcc gggcaggccg aattcgccga tgaagactgg cggcccataa
     5941 ggccaggcgc cggtgaacgt cggcccgccg tacgacatga tgcagttcag gtggttcgcc
     6001 acccgttcgg gctcctgcag cgaaagatta gttgcgttgt acgacgagta ggaaaccagg
     6061 tccatcgtga tttcgggcag caccgccgtg accatccgct gcacctcggg atgatccagc
     6121 acgacctgga tcgcgttgac ttccacgtag ttgtagactt cgacgccggt gacgtcgggc
     6181 aacgaattgc gcgcgtcctc cacggccttc tgccggttgc gcagccagcg ccggacggcg
     6241 tcgagccgcg ccggcgtcgg ttcctgattg ggatcgccgg cgtcgcggcc ctccagcagc
     6301 acgtggtcgg tctcccactg gccgaacaga aacaccttgc cggtgccctg atacgtcgtc
     6361 agcagatacc gcgcgaagtc atacagttgc tggtattccc ggtccagctc cgcctgggtg
     6421 atgttcgtcg tgaaatacca gccgtccatg gcgtaggccc acatcacgta gaacttgaac
     6481 ggcagatcga acacggtctg atacgccggt tcgttcagga ccagattcac gatggtgttt
     6541 ttctggttgc gcggcaggtc cggcagatcg gtgtaggtcg tacgccagct ctgccagccg
     6601 acgtcgagcc cgacctggaa cttgtaggcg tcggcgccga tctcgatcat ccgccgcgcc
     6661 tgctccacga tccccggttc atccgagtat ttttgatacc aggtgaaccc ctgggccgac
     6721 gcgttgatag ggaaataggg gtagactgta tcgtccgccg cgtgcgcggc ggcgccaacg
     6781 cacgccgcca gcagcagacc gcaaagccag gatcgcaagc gcgccgtcat cggcttccct
     6841 cccttcccat cctcaaaacg ccggccggcc ggctaaaggt taacccacca cgccggccgt
     6901 cagtgccggc cagacgcggc gaagttcggc tggtacttgg cccggatggc ctcggtcacc
     6961 tgattcagtt gagcgaactc gtggtcccgc cggctgaact cctcgcaatc gatcgcgtcc
     7021 agatacagct ccgaccggcg aacgttttcc cgcccggcgt actggccgga cccgttcgaa
     7081 agccaggcgg caagataatt ccgaaaatcg cggtccgaga cggcctgcgc gaattcgggc
     7141 gaatccagaa tcgtcaggaa caggtcgaac cgcggcgcct gcccctcggc cgacgcttcc
     7201 tccagccgcc cgcgcagcgc ggccgcggcc tcgtcccgaa tgccgcacgc cgccagcgcc
     7261 cagtccagaa actcctcgtc gctgacgagc gcgcggtatt catgcgagtt gacgatgcgc
     7321 gccagcagct cggagcgctt cacctggtcg aattccagcg ccagataatt cgcctccagc
     7381 tcggtcggat tccgccccag ccagacgcgc gtggcatgct tcagcaggtg catccggtcc
     7441 aggaagtccc gcatatcgtg gtattcctgg ttcttcgtcc catccagttc gatcagcgcc
     7501 gaattgccgt tctccaactg ggtgtagacc gaccagtaaa ggttgatcgg gctgccccag
     7561 ctcgcggccg atttgagcgc gatcagattg cgcgccggcc ggctcggcac cagcggcatc
     7621 gggatgccat attcgcccat gaacaccggc ttgccgtgct tccacgcgcc ggtgaacttc
     7681 gcgtgctcca gaatgtaatc gaaatggcgc cacgcgcgct cgggcagctc gtcggtgtga
     7741 ttcatgcagt tatacgccga gtacgagacc agatcgacgg ccacgtgcgg cagcaccgca
     7801 ttgatcagcc gcggcaggcc gtcatccagc accggcgtga tgatgttcag ctcggcatag
     7861 ttgtaaacct cgacgcccgt cacgtcgggc agcgaattgc gcccgtcctc caccgccttc
     7921 tgccggttgg ccagccactt ccgcatgtcc tccagcttgg cctcgcgcgg cgtccccttg
     7981 atcaggtcat tgtcctccat cagcatgttg tccccctccc agtggccgat gaggaagacc
     8041 ttgccggtgc cctgataggt ctgcatgaag tagcgcgtca ggtcgtaaag ctgctggtac
     8101 tcctgctcca tctccggctc gttgtattcg ccctcgccca gccgcagcgt catgtccatc
     8161 gggttgaccc acatgacgaa atacttgaac ggcatgtcga acacggtctt gtaggccggc
     8221 tcgttctcga tcaactcgac gagcgtgtgg ctcctgtgcc ggatttcgtc ggacagatcc
     8281 ttgtagacgg agacgtagtt ggcgtggtcg gcgtcggcgc cgagcagaaa cttgatcgcg
     8341 tcgctcccca tctcgagcat ccggcgcgcc tgttcgacca gcttcggctc gtcggtgaac
     8401 ttcttgatgc tgccgaaggc ctggctgccc gcccggagat tgaaatacgg aaactcggcg
     8461 ctctcgtccc cgtcgccttc aactgtaaac gacaggggtt cgctggtcgc cgggtcctgc
     8521 atgaactcgc cgctcagccc gctggcgtcg gtcgcggcga cggtccagta ccaccggccg
     8581 ggcgccagcg gggcggcggg ctggtagctt ctcccgggga cgccgtccag caccgtcgtg
     8641 caggccgccg agaagtacgg atcgcgtgat aaactgacgg tatagcgatc gccggtgaac
     8701 ccttcgggcg cctcccaggt caacgtcggc ggattgctgg ccgcggactg ctgcgcgggc
     8761 gcgaccggtc gtggagcgcc gggcgccgcc gccgcggccc cgcccgccac gatcttgatc
     8821 cagtcgacaa ccacttccac ctcgccgggg acccgctcgc gattgtcgcg gtccagctcc
     8881 agccgcagtc cgagtcgccg cgtgccggcc gggagtttct cgctcagatc gacggtcaac
     8941 agcgccgcgc tcggcgtgct ggacgtgttc ttgtgattga cgagcagatg cggaacgcgg
     9001 ccatcggcgt cggccagccc gtaaatcttc agccgggcct ccggcgagag ccgcgcaaac
     9061 cggacctgga gccgcggcgc cgccgccgcc tcgcactcga aatcgcgcgc cgtggtcacc
     9121 gaggctcgaa acgaggtctt tccgcgtaga ctcagccgcg cgttctctcc atcggagacg
     9181 aaggcggcag ccccgtcacc caggatgacg accggccact cccaggggtg atccatgccg
     9241 agatgatgct gtttgaagtc ctcaacccac acggcatccg ccctccccat cacgggcagt
     9301 agccattgcc ctatcagaat gaaccaggca atcgaacgaa ctcgcatgag acaacctctc
     9361 cagtgtcgga tgcaggccct gagagtggcc cgtcaaagag cagcgggtgt acccgaggca
     9421 catcaagcta attccatgtc gagaacatcg gccggtactt ggcccgaagc tcggcgttga
     9481 tccgatcggc caggtcggga tccagatcca tctgaatgaa agagcggctg ttcatggcct
     9541 ccaggaagcg ctccgaacgt ttcccatccc ccgaggcgga ttcctcaccc atcacctgcg
     9601 ccaggtagga cgcgaaatca tcctcgctga cggcttgcgc aaacgccgcg gaatccagta
     9661 tttccagcag cgcctcaaag cgcgtgagct cgccttgctt caggcgggtt gccagttcct
     9721 gcttcagttg cccggccgca tccgcggcga tgccacagcc atccagtatt tccgtcagga
     9781 accgctcatc ctcaaccatg acccggtact ctacggagtc aatcacccgg gccagaatat
     9841 cggaggtcgc gattcgatcg aactccaagg ccaggtaatt cgcctcgatt tcggtcggat
     9901 tgcgtccgag ccacgtccgc gtagcattct tcagggcgtg catgcgcgct ataaagtcgg
     9961 ccagatacca gtaatcctgg ttcttctcgc catcgaattc gacgagcgag gagtgaccgt
    10021 ccgcgtggac cgagtacgcc gaccagaaga gattgatcgg gctgccccaa ctcgccgcgg
    10081 attggatggc ggccaggttg cgcacgggac gtgccgggtt gagcgtgatc ggcaggcaat
    10141 actcccccac gaacaccggt ttgccgtgtt tccaggcgcc cgtgaagcgg gcatgctcca
    10201 gaatataatc aaaatgctgc cacgctcttt cgggcagctc atccgtatga ttcatgcagt
    10261 tgtaggcgga gtaggagatc agatcgacag gaacctcgga caggatcgcg ttgatcatgc
    10321 gtgggaggtt gtgctccagc acgggcgtga gtatgttgat ctccgcatag ttatagactt
    10381 cgacgcccgt cacgtcgggc agcgagttcc ggccgtcctc caccgcccgc tggcgattgg
    10441 cgatccagcg gcgcatgtcg ttgatcatcg catcgctcgg cgttccgttg atcagatcgt
    10501 tgccccccat caggacgttg tcgccttccc agtggccgat gagaaagacc ttccccgtcc
    10561 cctgataggt ttgcatcaaa aagcgcgtca ggtcgtatag ctggcgatat tcgctctcca
    10621 tttccggcgc attgtatttt ccccggccca gcgtgactcg catgtccatc gcattggccc
    10681 acatcgcgaa atacttgaac ggcagatcca gaacggtccg gtaggccggc tcattctcga
    10741 tcagctcgac cagcgtgtgg ctcttgcggc gaatctcgtc cgacatgtcc tggtagacgg
    10801 tcacataatt gacgtcctcc ccatcggccc cgagcagaaa cttgtaggag tcgcttccca
    10861 ttgccagcac gaagcgcgct tgctcgacaa tcttcggctc gtcggtgaac ttaatcgtgc
    10921 tgccgaagcc ctgactgccg gcccgcacgt taaaatgcgg atacaccgca tctgccttct
    10981 cttcggattc gggaatggta atggaaaacg gttttccggt cgcctcgttc aacagaaagt
    11041 cacccgataa ccctgacgtt gaggtcgcgc tgacgctcca ataccacaca cccgctccca
    11101 actccgcgtc cagtcgatgt cgcgtcgctt caatcccatc caggatggtc gtgcaggccg
    11161 ccccaaacag cggatcgcgc gaaatcgaca gcgtataacg attgtcgtca tacccttcgg
    11221 gcgccgacca ttgaagccat tgagcgtctt cggcgtcacc agaatccgca tcggcctcgg
    11281 tgctcgccga ccgcggcacg ccaggtacct gatccggcgc gcccggcaat cccaccttga
    11341 tccaatcgat gtagacctcc accaggcgtg gatcggcctt caggttactc ttgtccagtt
    11401 ccagcctcag gctcaggctc cgggtctcag cggggatctt gtcttgaaga tcgaccgtca
    11461 gcacctgcgc cgaaagcgcc tccgacgcat tctgatggtt caggaccagg cgcggctccc
    11521 ttccgtcggc gtcacacagg gcgtagacct tgattcgcgc ctcgggcgac agccggctga
    11581 tcctcatctg gagcagccgg gattgacctg cgtcccaggg gatcgtgcgc gcggtcgtca
    11641 cttggacgcg aaacgctgtc ccgctgcaaa gcgacagccg ggcgatctcc ccgtcggact
    11701 cgaagcggcc gcttccctga ccgagcagga ccaccggcat ttcccattcg tggtccatgc
    11761 cgagatgatt ctgtttgaag tcctcaaccc acacggtacc ggccctgccc ggcgcgggca
    11821 gcagcagttg gagcgccagc catgcccaga atattcgagc aacagacatg atcagccttt
    11881 cgtccgttgg gtttgaacaa tgcacaccgg cccgcgagcg gagccgttcg ctccgctgga
    11941 cgcgggccgg ccggcgcccg atgagcttat ggagcggtcg cgcaggcggt gtcgggcttc
    12001 agccgattga acgccagctt gcaggcctgg tagttcgtct ccgacatccc catgcacttg
    12061 taatcctcga cgtagccgac aaacccgccc tgttcggtgc cgagcagacc gaacagccgc
    12121 tccgcctccg cctggatatc ctcgggagtc ccgctgatcg agacggtctg ataactgatc
    12181 ggcagcatga acgcctgctt gccgcgcaac ccggcgccca cgcgcgggag atcgacgacg
    12241 ttgggctgcg agatgttgat cacatcgacc cctatctcgt ggaagtcctg gacgatcgcg
    12301 tcgatcttgc cgcagcagtg gaaccagacg gtcaggccga gttcgtgcgc cagccggaac
    12361 tgccgttcat aatgcggccg gaaccactgg cgccacatct ccggcgcgat cagcagcccg
    12421 tcctgatgcc cccagtcatc ccacaggatg atgccgtgca tgttctcctg cgcggcgacc
    12481 cggatcagct ccatttcgaa gtcgatgatc ttggtgagca ggaagttggc gtgctcgaaa
    12541 tcgaaggcga agtcggtcat cgcgttttca aacccgcgca gcccgcagta acaggtgaat
    12601 cccgacagat tgaggctggc cagccggtag tggtcgggcc attcggccac gaactggcgc
    12661 agcacctcgc gccgcttctc cggctgcagc tccgggaact tgtaagtctc caggtccgac
    12721 cactccgcga tcggatgccc cgtcatctgg cccatcgtgc cgtcgttgag cgactcgaac
    12781 ttgatgcccc actccgtcgt gcgggtctca tcctggaacg ccaggttcca gcagaccacc
    12841 acgtcgccct cgtgctgatc ccggttcagg tagagcacgg gcagccgatc ggggttctgg
    12901 aattgcaggg cgcgcaccac ccgttcgcat ttcgggctca gcggcgcgcc gctagtggct
    12961 tgaagctgca tgacgcactg actccatgtt gaatcgtggc tcgtttgagc aagtcccgtt
    13021 gcgccggcgc gcccgcggct cagaccggcg ccggcgcccg catccgcgtc gtctcccgca
    13081 cccgtagttc cggtttgatc cgcacgtgat accggccggc gtcctccagt tccgtctgcg
    13141 catggaacgc gtcgacgcgc tccagcagcc ggcccacggc gatccgcacg gtctgcacga
    13201 tgtgcagatc gaccgtcgtc acctgcgggc tcatctccgc cgacgcccgg atgttgtcga
    13261 atcccaccac cgccagatcg tccggcacgt ccaacccccg cttctgcagg acgtggatca
    13321 cctcgatcgc cagccggtca ttgccggcga agatcacctc gggcggcgcc ggccggtcca
    13381 gcaaccgatc caggcagccc ccgatatcgg cgatgttcgg gcaattgacc agatacccgg
    13441 gatcgtagct caggttgttc tccagcagcc cgcggcggta gccatcgagc cgaccggccc
    13501 atttccggtt gccctcgacc tccagatcgc ccatgaacgc cacccggctg tagccgcacc
    13561 gcttcaggaa gcacatcatg tcgaacgcgc ccgactcgtc gtccgagcgc acgctgtcga
    13621 tcccgatcgg ctccccctcg ccaatcatga ccaccgggcg ctcgtaatcc ttcagctcca
    13681 gatagcggtc cgcggctcgc tcctgcggcg gcgccgccac caggatcccc tcgacgcggt
    13741 agctcatgaa cgtctgcagg tatttgctct cgatctcctc cgaatcgttc gtcaccccca
    13801 gcagcacgct gaattccttg ctggccaggt gcgtcgtgat ctccgtgatc atgtcggcga
    13861 agaacggatt gaccacgttg ggaatcacgc agccgacgat attggtccgc cccagcgcca
    13921 gactgcgcgc gatgtggttg tgcttgtagc cgagctggcg cgcgacaagc tggacccgct
    13981 cgatcgtctc ggccttgagt ttgggatcgt tgcgcagcgc gcgcaccact gtggaccggg
    14041 ccagcccgac gatgtccgcc acatgctgcg tgctgatctg tttgccggcg gactggttgt
    14101 tccgtttgct cacctgatcc ccctgaccgg ccgctcgcgc ggtgcacggc cctggctgtc
    14161 ccggtttcgc cgctggactg ctgattcgcc gcgaattgtc tgctctccgt gcccctgtta
    14221 ttaggtgata acatagggtc ttgcttcgga ggagtcaatc tttttctcgc cgccgtttcg
    14281 gcctttatta ggtgataatc cgccccccca aacccccgaa cgccgggtcg gacccgtccc
    14341 agagaagccc ccaagttcgc cggcagttgc acgccgcccc gtcaccgggg gtggccccgc
    14401 cccctcgccg gtccgcccgc atccccgccc agacctccca cagcccgccc ggaattcggc
    14461 tcgcggctcc cgtctctcgt cctcggctct cgaagacccg ccattttttc ccctccgttt
    14521 gaatcggatg agcccgcctt tgaatgtatt tgctccctcc accggcccac cgcatactgt
    14581 ggagcgggag tgaccgggcg atgaagctct acagcctgct caacgaaaac cacgtcctga
    14641 tcggcgaagc ggtccagacg atggaggagg cgctgcgccg gatggtgcgc gccttcggcg
    14701 ccgccgtcgc ccccgaccag gtcgaccccc tcgtgcactc cctcctggcc cgcgaggaac
    14761 agcaccccac cgtcatcgac gaccgcgtct gcatccccca catgcggatg gaaagcctcg
    14821 acgtcttcct gctcggtctg ctggtgcccg agcacccgat cccccacccg aacccggagc
    14881 agggacccgt cgcgctgatc ttcatgatcc tggcgccgca aaccaagaac acgatgatgc
    14941 tgcagacgct ggcggcgatc gcgcggctgc tcaagtcgcg cgaaaccaag caggccatcc
    15001 tcagcgtgcg ctcggcgccg cgcctgctgc gctggatcga ggatagcggc atcgacgtca
    15061 agaagacgct gatggcgggc gacatcatgt cgccgatcac gcacacggtc cgcaccgaca
    15121 cggtcctcgc ccgcgccgtc gacgtcctgg tcgacgcgcc cgacgagggc atcccggtcc
    15181 tgaacgagca gggcaagctg gtcggcgagc tcaccagccg cgaactgctg cggctgggga
    15241 tgcccaatta cctcggcctg atcgccaacc cgtcgatgct cgacagcttc gaaccgttcg
    15301 aaaacttctt ccagcacgag aacagcatga ccgtgcgcga gatctgcggg cgcgatatcg
    15361 tcacggtttc cccttcggcg ccggtcgtgc aggtcgcgca tctgatgatg acccgccaga
    15421 aacggcggat ctatgtgctg gacgagggac agctgcgcgg catcatcttc cgcaagacga
    15481 ttatcgagcg ggtgctgcat ctgtgacgca cgattcgccc ccacgcagcg agaaagagaa
    15541 aatgctcgcc ggacagctct acctggcaag cgatcccgaa ctggtggcgg agcggctcca
    15601 ggcgcgtcgc ttgctccacc ggttcaatct cagcgcgccg gacgaatcgg cgctgcgcga
    15661 ggcgacgctg cgcgagctgt tcggccggat cggcccctcg ttttccatcg aaccgccgtt
    15721 ctattgcgat tacggctaca atatcctcgc cgacgacggg ctctacatga atttcggctg
    15781 cgtcgtcctc gatgtctgcc ccgtccacat cggcgccggc gtgatgttcg gaccctatgt
    15841 ccaggtctat gcggcgcatc acccgctcga tgcccggctg cgctgcgccg ggccggagct
    15901 cggctcgccg gtgtggatcg aggatcgcgt ctgggtcggc ggcggcgcca agatctgccc
    15961 cggcgtccgg atcggccgcg acagcgtgat cggcgccggc agcgtcgtca ctcgcgacat
    16021 ccccccgggc gtcgtcgcgg cgggcaatcc ctgccgcgtc ctgcgcgaca tccccgccgg
    16081 tgacctccgt tccccggaag aactgctgta gtccgtccct cgcccgctct ccagaagcgt
    16141 ccctcgtccc tcctccccct cgtccttcgt cctcnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnncccttg ctctcgggaa aaaccgaccg atcacgaaga cgagggacga
    16321 gagacgcgct tacgccgatg ggcgggactc tccctttgcc ccgacgggcc gggattcgtt
    16381 acgctgttgg catgagcgat cctcccgttg ccgccgccgc gcccgcgacc gccccgattg
    16441 cccggctccc cacgcgcctg atcaaccaga tcgccgccgg cgaggtcgtg acgcgccccg
    16501 ccgccgcgct gaaggaactg gtcgaaaaca gcctggacgc cggggccggc cggatcgaag
    16561 tctccgtcga ggaggatgcc ctcagctttt cggtccgcga cgacggcctc ggcatggccg
    16621 ccgacgacct ggtgctggcg gtcgaacgct acgccacgag caagattcgc gaactggacg
    16681 acctgcagcg gctgacgacg cgcgggtttc gcggcgaggc cctggcggcg atcgccgccg
    16741 tctcgcggct ggagatcgtc acccgcacgc ccgatgccct cgagggcctg cgcctgcgcg
    16801 tcagcggcgg ccaggcgtcg ccccccgagc cgtgcgcggc caatcccggc acgaccatcg
    16861 ccgtgcgcga tctcttcttc aatacgccgg cgcggctcaa atttctccgg gcggccgtcg
    16921 ccgaatgggg acacatgctg caggcattcg tgcgccagag cctgacgcgc cccgacgtcg
    16981 ccttctcgat ccgctggcgc cggcgcccgt atctcgacct gcccgcgggc cagtcgatcc
    17041 gcgaccggct ggcccaggtc ctccccaccg gcgcggggca ggatctgctc tccctcgacc
    17101 tgacgctgca cgaggtgcgc gtgaccggcg cagtcaccgg accgcgcacg acccgccgcg
    17161 accgccggca tcaatacgcc ttcgtcaacg gccggccgat cgtcttccgc ccgttgatgt
    17221 tcgcgctcga agaggcctat cgcgggctga tcatgacgca gcgctacccg atggcggccg
    17281 tgatgctgac gctgccgggc gagctggtcg acgtgaacgt ccatcccacg aaggagcagg
    17341 tccggttccg caacgaggcg ctggtcgcgg gagccgtgca ccgggcggtg ctcgaggcgc
    17401 tgcgcgcggc cgacctcgtc ccgcgcctgc aaatgccccc acccggagcc ggcctgaagc
    17461 tggccgcccc gccgatgccg aaggacggaa cgcgcccggc gacgcggacc gccgcgcccg
    17521 ctcccccgct gcggctcgac cagccggacc tgaccgatgc cagccgcgac ggcgctcagc
    17581 cgggcgacca gctcgacttc atccccgggt ttggcctgcc gggagaaagt caggggaccg
    17641 aaggaactaa tccgccgccg tccccgccgc cgccgtcaaa cacgccggcg ggaccggccc
    17701 ccgtacccta cgccgaaccg gtcgcgcgcg acgaagcctc actgctcgaa cgtcttcgcc
    17761 ggctgcctca tcctccccgc atgctcggtc aggtggccga aacctacctc ctcgccgagg
    17821 cgggcgcgga ggggatgctg gtgatcgacc agcacgccgc ccacgagaag atcctctatc
    17881 tgcgttacct gcgcgcggcg gtgcgccccg acggcatcca ggtgcagccg ctgatgatcc
    17941 cgcattcgat cgacctggcg ccggaggacg agcccgcgct cgaaaccttg cgcccggcgt
    18001 tggcggaggc tggcttcgag ttggaaccgt tcggcggccg cacgtggctc gtccaggcga
    18061 tcccggtcct cttcgaccgg ctcgacgtgc cggcgttcct gcgcgacctg atcgacgacg
    18121 tcggccaggg cgacctgccg cgcgagatgc gacgcctgcg cgagcggatc tgcgcccgcg
    18181 ccgcctgccg cgcggccgtg aaggccggag accgtctggc gcccgaggag atgcagcggc
    18241 tcctgcacga cctgctcgga accgccgacg cgctgcgctg cccgcacggc cggccgacgc
    18301 tgctgctgct gacgcgcgac cagctcgacc gccagttcgg ccggctcggc tgaccccgcg
    18361 ccatcgccgc aatcggtatc gaaatcggta tcgctctcga tatcgaatca atcttcacgc
    18421 cgcgaaccga cgccgttagt ccgagtattc ctcaacggtc gtttcgtgcc gttcgatgtg
    18481 gcgctcactg cttgacccga ccgtgccgcc gtcggattcg gtatgaaccg ttgaggactt
    18541 gcgttcgact tgcacgctct tgtcgacgcc atcgtcatcg tcttcatagt aggcacatga
    18601 cgagagcatc agcggcgccg ccagcatcag actcgccagg gtaagctgtt tccaggtgcg
    18661 catgggggaa tcctccattc cagatgggag accgccggat gcggcggctg gagaggaaag
    18721 gagagccatt cgcatgccac accgcaggaa gttgttgccc cctgaacgcc ctcgccatca
    18781 accgccgaat cacctcagcc ctttgtgtcc ccttgtgtac ctcgtgtccc ttgtctccct
    18841 tccgtccctt caccctcagc gaaatccggc cttcattgga aatacccacc cgaacaagtt
    18901 aaacttgcgg caagcccctg cggaacgcgt aaccaggaac cgggagtcgg attccatgga
    18961 atcgaccagc aacgaaccac cggcgggccg cccgcgcatc gtgatcctcg gcggcggatt
    19021 cggcggcgcc tacgcggcga tgtatctgga acgcgccctg cggccggacg aggcggagat
    19081 cctgctgatc gaccgcaata attacttcgt cttcacgccg ctgttggttg aggccggcgt
    19141 cggcggactc gaaccgcgcc acaccgtcgt ctcgctgcgc cgcttcgcgc gccgctcgcg
    19201 gttccgcatg gccgaggtgc gcggcgtcga cctggcgcgc cggatcgtcc ggattcagcc
    19261 gcccgatccc ccccaggaac tggaaatccc ctacgaccac ctcgtgctgg ccatggggag
    19321 cgtcacgctg ctgccgccga tccccggcct gcgcgagttc ggctatgaaa tgaagagcct
    19381 gcgcgatgcg gtggcgttgc gcgaccgcat gatccggatg ctcgaggtgg ccgacggcat
    19441 cgatgacccc gccgagcgcg cggcccggct ccacctggtc gtcgtcggcg cgaacttctc
    19501 cggcgtcgag gtcgcgggcg aattccatgc cttcatgcgc gaggcctcgc gccgctaccc
    19561 caacgtccat ccccgcgagt gcaacgtcac gctggtggaa ttgaaggaac ggatcctgca
    19621 ggcgctcgac cccgacctcg cgcgcttcgc cgcccggaat ctgatgcagc gttcgatccg
    19681 cgtcctgctg cgcacgaccg tcgccgaggt gcatgcggat cacgtgctgc tgagcaacgg
    19741 cgagcggctc gctgcccata ccgtgatctg gtgcgccggc atcacctcca atccgacgct
    19801 ggccggcgtc gagggcctgc cgcgcgacgc acgggggttc gtcctctgcg agcccgatct
    19861 gcgcgcgtgc ggcctgccgg atgtctgggg catcggcgat tgcgccgtca atcccgatcc
    19921 gcaggggaac gcctatccgg cgacggcgca gcacgccgtg cagcaggcgc gcgtcctcgc
    19981 gcgaaatctg cgccgcgtcc tgcgcggcca gccgaccgag ccctgccgga tccgcaaccg
    20041 cggctcgctc gccgcgctcg gctgccgcac cggcgtcgcg cgcatcgggc cgctcaagct
    20101 ctcgggctgg gccgcctggt tcatgtggcg gacggtttac ctgctgaaga tgcccgggct
    20161 cgcccgcggc gtccgcatcg cgctggactg gacgctggac ctcttcttcc ggcgggaata
    20221 cagcgccctc ggcctgcacc agcgcccctc tccccccgcg cccaatgatt cggatccctg
    20281 acggctcggg cggcgccgcc ttcaggcccc atcattcccc aaatcgtaat ccgtccacgg
    20341 attgcatcat gaccgttaag cttctgattc cgatgcgggc ggtccaatct gttgacggga
    20401 tcagtccgcg gagcggacgg ccggaattaa gcgcagggtg acggccgcag gccggatccc
    20461 tgcgtcgccg tccccaacgc atcgaacccc tgcagggggt ggccggagcc cgggccggcc
    20521 ttccgttgcc ccgcctcagt ccggctcgtc ggtgatcagg cgcgccaggt ccgtcggcgt
    20581 gttcacattc cggatgacgg ccgggttggg cagatccacc gtcacgacgt cggccgcgtg
    20641 ccggcgggcg agctggttga gcccgtcgtc gcacgccggc gagtaaacga aatcgcgccg
    20701 cgcggcggga aagatcagcg ggtgaccgcg ccagccgcca tggccggcaa tcacgatgcg
    20761 cgcggggtcc cgccggtagg ccgccgcgca cgccgcaatg tcccgcgccg tcaggccggg
    20821 ctgatcgccc ggacagacaa caaagccatc ctgggggccg atcggccgcg ccgcttccca
    20881 ccaccgcatc ccgagccgca ccgaatcgat catctccgcc gccggatcgg gattgacggc
    20941 cacggccgcg cccagcccgc cgagccgcgc cgcgagcgcg gcgttcgtca cgatcagcac
    21001 gccctcgagc cccgccgcga caagcggctc cacgacgcgc gccagcatcg gctgcccccg
    21061 cacgtcgatc agcgatttgg gccgccccat ccgccgcgac cggccggccg ccgggatgat
    21121 ggcatagagc tgcgccgggt ggctggagcg gctggtcgcg ttcatgttac gatgatacgc
    21181 gatcgcgacc cgcgccgaaa gcccgccgcc ggccgtcccg tcagcgtcag ccccaaaccc
    21241 gcggaggatg ttcatgagcg atctgcttcc cgtgcttgac gcgctggtgg cggagcacgc
    21301 cgccggccgc cgctgcgtgc tgtgcgccgt cgtcggcaag aaggggtcga cgccgcagcc
    21361 gcccggcgcc gcgctcctgg tgcgcgagga ccgcacgacc gtcgggacgc tgggcggcgg
    21421 ctgcgtcgag gccgaggtca tccgccgcgc ccacaaggaa tcgctcgcga ccggcgaagc
    21481 ccgccggatg gcgttcgtgc tgaaccatga ctggggctgg gatgacggtc tgatctgcgg
    21541 cggccggatg gagatcggcg tgatgccggt cgcgccggcc gccgatgtga cgccgtatcg
    21601 cgaggcgatc gccgccgcgc gcgcccgccg cgaggcctgg tttccgctcg tcgtcgcgga
    21661 ggagggccgg acattcgcct atcgcattcg catggaggcc ccgccgacgc tgctgatcgc
    21721 cggggccggc catgtggggc aggcgctcgc gcggctggcg acgagcctgg atttccatgt
    21781 cgtcgtcgtg gacgaccgcg aggattacgc ctccgccgcg cgcttccccg ctccgatcga
    21841 acgcgtcacg cggagcatcc ccgccgccct ggccgactgg ccgatcgacg cgcacaccta
    21901 cgtcgtgatc gtcacgcgcg gccatcagaa cgaccagcgg gcgctcgagg cggttatcca
    21961 ccgcccggcc gcctacatcg gtctgatcgg cagcgaacgc aagcggcggc tcatcttcga
    22021 cgacctgatc cgccagggcg tcgcgccgga acgactggac gccgtgcaca gcccgatcgg
    22081 cctgccgatc cacgccgtca ccgtgccgga aatcgccgtc agcatcgccg ccgagctgat
    22141 tcagcaccgc cgggcgacgc cccccgtgct ggtcgacggg ccgcatgtca tcggcgccgc
    22201 cgaccgcccg ccgagcgggg ccttcgccgg cgatgccggc tgatgcaacc gcgcgccttt
    22261 actccgccgg tgcgaattct atctgaatcg acggcccctc acgccggatt ttcattagct
    22321 tttgctcgtc gtcgtaccac cagcccgtat cgtcgctgaa ccactcctcg atccgtggga
    22381 gttcggcgtt gtcggcggtc gccacgcgcg tcggcgcgcc ctggacgacc agcgtttcct
    22441 cgcccccgcc gatcgcatgg tatcccaggc ggttttcgcc gtagtccacc gatttgagca
    22501 gaccagtggt ttgcagcagg tggttttcac ccgccggcgc ggtctccggc agcgccgaca
    22561 tgatgcggca gaactggtcg ttgaacgaga gcaggatcgg gcaggcgatg gcgaaatagc
    22621 ccagcccctg ccgcaccttg ccgtccgaca tctggcacca ggtcgtcgag ttggccgtct
    22681 cgagcgcctt gatccgatat tccggtttcc ccgtcgcctc gtacagcttg gcgtacattt
    22741 cggccaggcg cgcctgatgg tgcgtcagca cgacgatgaa cgtcgcctgc tcgatcaggc
    22801 cgggaatgcc ctcatgaaat ccgcacggca ccatcaggtg ctcctgaatc caggccagga
    22861 tgccctcggc gatctccacg gcctcgggat actcgtcgcg ctggtcgagg agcatcatcg
    22921 ccgtttcctg cgcgatccac tgatcatacg tcgccgaatc gctcggcacg tcgccgtaca
    22981 gcccgatcca cttgttcgtt ttcaccggat tctccagaac ccactggaag gcgcgccggc
    23041 tgcgctcgag atcctccgga tcgccgctga ccttggccag ctgttcgaag aaccaggagt
    23101 ggaacaattg gctgcacgcg taatcgacct ccacctcgcc cgtctcggca ttgacgcgga
    23161 acggccagct gccatcttcc ttctgcagcg ggcgcagcgt cgcggcgatc ttcagcgcgg
    23221 cgtccaggta tcgcggctcg tccgtcacgg cgtaaagcct gaggaaggcc agccccatgt
    23281 aggcgctctt gtcgatctcg atcccccatt caacggtctg gatcgttccg tcggggccat
    23341 aggtgcccgc gctcatcggc atgtagggca gggcccagtc ggcgggcgag cggttggcga
    23401 tgtgccagtc ggcgagcgtg cgcgcctcgt ccagaatgtc gtcgttgccc gaataaaggt
    23461 aatgcgtcac cgcgccgcaa atatacagcg aatgattaaa ggcaaattca ttgagcgtca
    23521 tccagagctg atcgtcggaa cgcatgaaca ttgacagaat gtaaggcggc aggtgccgga
    23581 ccgactcggg cgcgagcgac gtatcgatca cttcctgcga ccgggcggcc caggccgtca
    23641 ggtcgcgcag caccttatcg taggactgcc gcggcagcat catgccggat gcatcgcggc
    23701 ggccgctctg cggcgtgatg atgtgcgcga agtcccgcag cggcgtgacc gtcagcccgc
    23761 tgaaccgcgc ggcggcgccc accgacatca ggccgacctg gccctgatcg gggtcgtctt
    23821 cccacagcgg ccaggtgccg caatcggccg gcggcgtgcc gagaaaatcg aagctcacca
    23881 ccggctcgcc gtcgacgtag cccatcaccg ccgtgccgtg aacgtcggcc cgcagcgtgt
    23941 gcggctgcgt caggtcgagc ccctccagcc aggcttggtc gcccgtcagc ggaagctccg
    24001 cgttctggta gcgcaggatc gcgtacggcc cgctcttgcg cgcggcgagc gagaagacca
    24061 ggtagtcggt cgcgctttcg gcctgcagga ccagcccggc ttcggacccc acctcggcgc
    24121 cgcccagcgt cacctgcgtc tccgcggtgt aatcgttcca atcctcctcg ccggccagca
    24181 ggacggccgg ttcggcggcc ggttccgttc gttcgatgcc gctctcgccg gcctgccacc
    24241 cggcgggcag ggcgtcggcg gcgaggctga acccgccctc cggcacgccg gcccgtgccg
    24301 cggtcgcggc gccggccagg atgatcagcg gcagcagaag ccgccgcagc aggatgcgct
    24361 ggaagagcac cggtgattgc atgcgtccgt cactccatgg gatgttgaat caggctgaat
    24421 gaatcgcgcc gataaaagcg attcattgtt ccaatacaat ttcgatggcc ggcgcggcgt
    24481 gccggatccg cagttgcccc gtctgctcgt cataccacca gccgtcctcg tcgaagcccc
    24541 agctccgcag ccggttaagc ggctcgcccc ccgccgtcac ctggcgcggt cgggcgcgca
    24601 ccaccagcgt ttcctctccg ggccccagcg tcgcatacga aacgccgtcg tcccggtagg
    24661 cgatccgcgt caggtcgctc gtgttgtgca gcaggtgatt ctggtcgcgc ggggccgtct
    24721 cggggatgct gcccatgatc cggcagagct ggtagttgta ggacaggacg agcggcgaat
    24781 cgatggcgag gtaccacaga ccctggcgca tcttgccgtc ggaaacctgg caccaggtcg
    24841 tcgaattcgc ggccgcgacc gctgcctgct tgtaatcctc gcggcccgtg gcttcataca
    24901 gtttggcgta cagttcggca atccggaact cctggatcgt ctcgacgacg gagaatgagc
    24961 cctgttcgag cagacccggc acgtcgggat ggaatccgta atcgacgatc aacgtgcgtt
    25021 tgagatagtc gagaatatcc tccgcgatcc gcaccgcccc cacgaattca tcgcgatggt
    25081 cgatcaggta aatcgcggtc tcgatcggca cccattgatc gtgcgattcc gccccgatca
    25141 gcacgtcgcc atagacgccg aaccagcggt tgtccttgac cgggccgtcg agcagccatt
    25201 gcaacgcctt gcggctgcgc tcgcggtctt cctccttgcc atcaatttcg tagagtttat
    25261 cgaaaaaatg gacataccac agttggctgc aggaatacgc ttccttgatc tcgccggtca
    25321 gcgcgttgat ccggaacggc caattgccct cctcggattg atacttgcgc agaaccgacg
    25381 ccaggtcgcg cgcgccgttc agatagcgct cgtctcccga ggccgcatac agcctgagca
    25441 gcgcatagcc catgtaggcc gatttgtcca tctccaggcc ccactccatc ccttcccacg
    25501 tgccgtcttc ctgccagttg acgacgctct ggatcaggtg cgggactttg gcgtcggcgg
    25561 ggacccggtt ttcgatgtgc cagtcggcga gatcccgcgc aaactgcaag acgcgttcat
    25621 cgcccgaata ataataatat tgtacggcgc cgaggatgct gatcgagtgg ttgaagccgt
    25681 attcgccccc cagcagccag aatcgattgt cggtcgaaag ccagttggtg agcagatagg
    25741 gcggcagatg gcggagcgat gccggcgccg gcgacggatc gaccacctcg gccgaggtga
    25801 ccatccagtc gttcagcatc cgcatcgtct catcatacga gcgcaacggg agcagccggc
    25861 cctgcgcgtc gcgccggccg cgcagcggcg tcggcagcgg cgattcgccc ttcagcggcg
    25921 acacactcaa atcttcaaac cgcgccgcgc agccggcggc gatcacaccg acccgcccgt
    25981 gcgtcgggtc tgcgggccac acctggccga aatgatcgaa cggcgggggc gtccccatga
    26041 accaatagcg cgccgccggc tcgccgtcca ggtagcagac cacttcggcg ccctcgacgt
    26101 ccgcccgcag cgtgtgccac tctcccgcat ccagatcctt gtggcgcgag acgtcgcccg
    26161 tgaagacctc ctcgatcagc ggcgattcgc cgcggtagcg ctcgatcgcc gccgcgtcct
    26221 gaaagccgag gacggcaaag aacccgccgt ggcggcgttc cagcgtgaaa tacacatagc
    26281 gcgcggcatc gcgcgcctgg aagaccaggc cggcgcgggc gcccgtcgtc ggctcgtcga
    26341 agcgcacccg cgtctcgacc gtgtagcggc tccagctctc gtcgccgacc agcagcagcg
    26401 cgggctccgc ggcggcgccg gtgtgagcga tgccgtcgcc gcgcgcgacc cacccgccgg
    26461 cttggccctc tgcggccgcg agcgcgtcgg ccgtcagcgg cagcgggcgc tccggttcaa
    26521 cccaggccgc ggccgcgggc cgcatggccg cggcaagcaa cagaatggtg atcgcgatgg
    26581 gcaggccctt catggcggct tgctcctcgt tggactggct ggggcgattc ggactccgcc
    26641 ccccgccagg cggttcacag ccgggcatca ttgggtccag gggcgtgcga aatcgtttac
    26701 tctgggcatc ctagcccctc cccaccgccc ctgtccagcg cggaaaatgc cctctgcccg
    26761 atccgctgac ccgccagcgc acgcggtccg ggccgtaacc cgccggaacg gtcggttccg
    26821 tcggattcac ccgcctcagt cgtggaatgg atttcgaatg ggtgagccta gcgcggcgcg
    26881 gcgtcgggca ggccgccgtc gacggggatc gtggcgcccg tcgtcggcgt ctgccgcgtc
    26941 gcgaaaaaca acacggcgtt ggccacgtgc cgcgccgtca cccgcgcctt cagcagattc
    27001 cgcgaacggt aatattcctc cagacccgct tcatccaggc cgcgggcttt catccggtcg
    27061 gggccgaccg tcgcccacag gcccgaccgc acctcgcccg cgctgaacac tgcgtcgggc
    27121 gccaccatgt tgacgcggat atcgtgctcg gccagttcca acgcggcgat gcgcgcgagc
    27181 tgatgcgccg ccgccttcgt cgcgctgtag gcgccgaacc gcgccccggg ggcgaatacg
    27241 ttcttcgtcg aaatcagaat cacgtcgccg ccgatcccct gccgcttcag ccagcggccc
    27301 gcttccgcca gcgtgttcag cgtcccctcc acgttcacgc gctccagccg ctgaaaatcc
    27361 gcaagctgca gttcatcgag ccccgccacg tgcgcgatcc cggcgttcac aatcaccaga
    27421 tcgacgccgc cccagacgag agcaagcgcc gcgaacgcgg cgcgcacccc ctccggcgcg
    27481 gtcacgtcca tgggaattcc aatgatatta tcctgaaatt cccggccgaa cgattccacc
    27541 gtcgccgtca aggcatcccc gggcaggtcg gttgccgcga cgcggcagcc cttctccagc
    27601 agcccgcggc agagaccgtg gccgatcgcc cccgccgcgc cggtcacgag cgccacccgg
    27661 ccggccagcg gcgctgccgc cgtcgccggc acgtcctcga tttcatacac cgcctgctcg
    27721 ccgatctgca cggcgcgcgg gcgcatattc ttggattgaa tgaacgcggc cagcgcttcg
    27781 gcgattcgcg cgcgggccgc cgcctccgct tcgcccgcga ggccggccgc caggatcagc
    27841 ggcgacatca ccatcccgcc ccgccggcgc atcaccggcg gctgccacgg ccggtcctcg
    27901 tcgcccgtcg caacggccag ggccccgcgc gccacgccga ccagattcgc cagcggatgg
    27961 tccaacgccg ccgatgggtt gagcatggct tccacggaac gctctcgcct aaccaacgga
    28021 aagcttggga aaagcctgca cgacggcgcg cacttcccag gcggaataga caccgcccgc
    28081 gacatacgga tcgcccgcca cccacgcgcg cgccgcgtcg gggtcctcgg cctcgaagat
    28141 gatcagcgcg ccgttggatt tccccgcctc atccttcagc ggcccggcaa agcggatgcg
    28201 cccggccgcg tcctgcgcgg cgatgtacgc cacgtgcgct tcgcgatggg cattgcgccg
    28261 ctccgcgccg tccggtccgt cggttgcatg gagcacaaac agcatgcgaa tcccccccgt
    28321 catccaatgc ccaccggctc gcgcttctcc cgcgcctgct cgttcttcag ttccgcgtag
    28381 acctccatgg cctccttcgg agtgtagcct tcgtgcacga tcttgcggat cgcctgcagc
    28441 atcgccatcg gcgcgtcgct ctggaaaatg ttgcgcccca tgtcgacgcc ggcggcgccc
    28501 ccctgaatgg cgttgtaggc catcgtcaat gcgtccagct ccggcagctt cttgccgccc
    28561 gccatcacga tcggcaccgg gcaactggcc gtgaccgtct cgaaatcctc gtcgcagtag
    28621 taggtcttga ccatctgcgc ccccagctcg gcgcagatgc ggcaggccag ccgcagatac
    28681 ttcgcgtcgc gcaccagctc gcgcccgacc gccgtcaccg ccatcaccgg aatgccgtag
    28741 cgcagccccg catccaccag ccgcgtcatg ttgtggatcg tgcgcgtctc gtgttcgccg
    28801 ccgacgaaca cctgcagcgt catcgcggcg acgttcatcc ggatcgcgtc ctcgacgtcg
    28861 accgccagct cctcgttgga cagctccttc aggatcgacg gaccgccgct gcagcgcatc
    28921 acgatcccgc gcggatacgt cggcggaatc gtcgaacgca ggatcccccg cgtcagcatc
    28981 agcgcgtcgg tctgctcgag cagcggaacg atgttcaggt ccacgcgctc caggccggtc
    29041 gtcggcccct gaaaatagcc gtggtcgatc gcgagcatga cgactttgcc cgtctcgggc
    29101 cggaagatcc gcgacagccg gtgttgcatc ccccaatcga gcgcgcccgc ccccttggcg
    29161 aaaaagccgt gcgtcggctg cgggatttcc ggatggaatt gcttggcttg cttgttgtcg
    29221 gcttcaggca ttggccgtgc tcctcattcg aactgcattc ctcactctaa aacctgtccc
    29281 tcgtccctcg tccctcgtcc ctcgtccctc gtcctcactc aaatatccgt ccctcgtctc
    29341 tcgccctcgt aatcgccccc aaagtccatc ccccaaaatc ggctcccggc tctcgtccct
    29401 cgtcctcggc tctcgtaatg tatctcgtgc atcccggcag ggatgccagt acctgaccgg
    29461 tgtccgttcc gcatcacccc caccggcgtc ttaacccctc gctgagagac gccagcgcgc
    29521 gcgaccgcgc tcagcgctcc gccggcgccg ctttcaacac gtccttcaga tcctccgtct
    29581 tcccgtattt gaacaggtaa tgatgccgtt cctcgcgcca caattcctcg gggatcggcc
    29641 gcacgccgcc gatcgcctcg gcctggatga agatccgcgc cgccttctcg acaaactggc
    29701 agcagatgac cgcctcttcc agcgtccgcc cgccggcgat caccccatgg ttcgcgatca
    29761 gcaccgccca cgcctcgtcg cccagcgcct cgcacacggc cgccgccagt tccttgtgcc
    29821 ggccggccgg cacatagcgc gaacagcgga cctccccccc cacgaccatc gccatgtcgt
    29881 cgatcagcac cgggatggcg cgctgggcgc aggcgcaggt ggaagcccag gtcgaatggc
    29941 tgtgaatcag cgcccccaga tcgggccgcg cccgcaggat ctggcggtgc atttccgtct
    30001 ccgacgtcgg cgtgcgcgtc cccttcacct gccggccgtc gagcgacacg acgcacagat
    30061 cctccggcgt cagatcctcg tattccaccc gcgtgggcgt cagcagcaat ccttcctcca
    30121 cgcgcaccga catgttgccc caggtgctga tgaaatagcc cagccggtcg cgcatcagaa
    30181 agcaggtgtc gatcacctgc cggcgcagcg cggtcgtgtc ggcgaagggg gcgacgggcg
    30241 gaacctgcaa ggcaacacct ctccacgggg ccgtgccgcc atcggccggc agcctcgatg
    30301 attctgagac agcacgggtg ggacatgcaa gcgggttcgg ccggcagcgg ctgtctcgtc
    30361 cttacgtccc ctccctcaca tcgcactcgc cggctctcgg gttccgctcg tccggctcct
    30421 ctggccttgt agaatctccg ccttggcccg ctcgcagatg tcctccatcg ccgccatctg
    30481 ctcctcgctg agcggccgcg ggtgatgcgt cgccagaatc gcgcgggcct tttccgttgc
    30541 gatctcgatg atgtccctgc ccccgcgcgc cagccaactg tcgtacgtgt tgcggtcgaa
    30601 cagttccgag gcccagaagt tccggcggct ttcaatcgcc gtatgcggat gggtcagaaa
    30661 gacgccgccg atccgctcac gtcctcacca cactctggtg cgtcctctga gcatccgaag
    30721 ctcacggtcg ctgccacctt tgccaaatcc tcgtccgtga tctgcaccaa gtgaccgtat
    30781 cgtttagcca tcgtcatcca cttacacccc agcaaccgcg ctaccgctac gagtggcact
    30841 ccctgtatac caagtgagag gcgcaagtat ccctgaaggc tggcacctga tagtcaggga
    30901 ggtccgccac ggcataggcg cgcttgagca cttcgcgcag cttgccgtcc tggcggaaca
    30961 caggttcatc agacgccccg cgcggcaaaa gatcgaacaa cgaaagcaac gggtcgaaca
    31021 taggtatcgt gcgagtcagt ggcttcatcg ccacgctagg cgaaccaccg cacaaccgta
    31081 tcgtccgcgc ctctccagca ccactgcttc gatgtgctcg cgcag
//
LOCUS       DAOPCO010000017        30336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1652_length_30336_cov_4.740134, whole genome shotgun sequence.
ACCESSION   DAOPCO010000017 DAOPCO010000000
VERSION     DAOPCO010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 30336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30336
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1652_length_30336_cov_4.740134"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLS90_02550"
     CDS             <1..493
                     /locus_tag="PLS90_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01314.1"
                     /translation="RWDPFQNAGVKQVWVHLMNNFNDRGTSNEGRLLAVPVNIRTKAR
                     LQVGRVWAEATPDEIDDNRMRLLAEVKNTGETAARGVVVSFYRSAIQTEENELGEVML
                     NQIAGGTSGIATLLWEFDPRRDLVRGTQLPSPTVRVWLKGSSQRISSVPDATDDGSSP
                     LEE"
     gene            complement(566..781)
                     /gene="rpmE"
                     /locus_tag="PLS90_02555"
     CDS             complement(566..781)
                     /gene="rpmE"
                     /locus_tag="PLS90_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPK01315.1"
                     /translation="MKKGIHPEYKLTTIRCACGAEYKVRSTRENQRLEICASCHPFFT
                     GRQKLVDTAGRVEKFNRRYSKFQNAPK"
     gene            complement(1103..2527)
                     /locus_tag="PLS90_02560"
     CDS             complement(1103..2527)
                     /locus_tag="PLS90_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPK01316.1"
                     /translation="MHHASLPPLNLDDLRVRWRERGGYRDIFWIAGPLILSTSSLTIQ
                     QFIDRLFLSWHSEAEFAASLPASWFSFVIIGFFMGTAGYANAFVAQYNGAGRPDRVGA
                     AVWQAVYFSIAAGVIIIAAIPLTSRVLVWADHPPDVLAHELTYFRITCYTGGFITLSS
                     AVSALYTGLGRTWTVLLVNVASTLVNIVLDYAMIFGHWGFPEWGIAGAAWATFVSAAV
                     NSAVYLVLFLNAYNRRRYATGRRWRLEWALLKRLLRFGAPSGGQIMIDILSFAAFTLL
                     VGRLGMTELTATNLAFQINMIAFMPMIGLGIAVTTLVGQHLGENHPERAARATWAAFH
                     VAFCYMGLCALLFVLIPNVFIRWFAGEAPAAEFLQVSRVAVVLLRFVAAYCLFDVMVI
                     IFSSALKGAGDTRFVLLVHLTLAWTIMVIPTWLFIERGWGGVYWAWIFATTFVNVAGL
                     VMLRRFLKNHWVTMRVIETAPAPV"
     gene            complement(2635..3393)
                     /locus_tag="PLS90_02565"
     CDS             complement(2635..3393)
                     /locus_tag="PLS90_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01317.1"
                     /translation="MNRPLALLAALWLLLSTAIPAGVAVAEDSQPATPEAAAAPTESS
                     PKAKKGNPYPLTTCPVSGEPLGAMGDPVVIEYEGREIRFCCPSCEAPFKEDPTSFLIK
                     INEQLIEQQKPTYPLETCVVSGEKLGGMGEPFDYVHDNRLVRFCCRACVAEFQSDPAQ
                     YFEQIDQAVIEAQRADYPLATCIVSGETLGDHGEPVDYVLGTQLIRLCCPACVAELEK
                     NPAKYLKQLRPDEEGTEATEPAEGAETMEGSAGA"
     regulatory      3838..3929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4172..5050
                     /gene="lipA"
                     /locus_tag="PLS90_02570"
     CDS             4172..5050
                     /gene="lipA"
                     /locus_tag="PLS90_02570"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016780334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPK01318.1"
                     /translation="MESARLPHWLKRPLGGGPLYARTRGAVDSGRLHTICEEARCPNR
                     GECWSRGTATFLILGDRCTRRCGFCSVKPGRPRGRVDGDEPRRLAEAVAHMRLGYVVL
                     TSVNRDDLPDGGAAHFRDCIRAIRAAVPGIRIELLTPDFRRCAGEALATLASEAPLVW
                     GHNVETTPRLYAAVRPGSNYQHSLDLLRRVALLPGVAAKSSLMLGLGEREEEVLRVLD
                     DLARVGIERLTLGQYLRPTPAQLPVVEYIHPDRFHRLADEARRRGIRWVMASPFTRSS
                     YFAEAERPGGKAGIHA"
     gene            5047..6150
                     /gene="gcvT"
                     /locus_tag="PLS90_02575"
     CDS             5047..6150
                     /gene="gcvT"
                     /locus_tag="PLS90_02575"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPK01319.1"
                     /translation="MTTPSLLRTPLHAAHIAAGARMAPFAGWEMPLHYGSQIDEHHAT
                     RKACGLFDVSHMGQVRITGCDALKLVQAIVTRDVAHLGDGRSAYCAMCNRAGGLLDDL
                     IVSRLAMGDYLLVVNAGTYEGDLAQMHAVEREFGPFDARIDGCRDDWAMLAVQGPEAP
                     AVLARVLGGGLDPPAMRMISVEWRGQAFWLSTTGYTGERGAELIAPVEMARDLWDALM
                     AAGARPVGLAARDTLRLEQAFNLSGQDFTAQNHPFEVRLGWVVDLDKPDFCGREALRA
                     IRAGVCPRQQCGLLPEGRRIPRHGARVLHQGKQVGVVTSGGFSPTLDRPIALGLLQSQ
                     MVKSEEPLEIDLGRGATVPAVITPLPFLRAQPL"
     gene            6163..6549
                     /gene="gcvH"
                     /locus_tag="PLS90_02580"
     CDS             6163..6549
                     /gene="gcvH"
                     /locus_tag="PLS90_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPK01320.1"
                     /translation="MQPETLRYRATHEWIEASGAERRVGISSFAQEQLGDIVFVELPT
                     DLPRDVAAGDEVLVIESCKATSSVYAPLAGRVTGVNQRLADHPERVNQDPTGEGWLFT
                     LEVAPGADESELLDAEGYRRLCEAEG"
     gene            6602..9502
                     /gene="gcvP"
                     /locus_tag="PLS90_02585"
     CDS             6602..9502
                     /gene="gcvP"
                     /locus_tag="PLS90_02585"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPK01321.1"
                     /translation="MTVAPLPSVSLQPLDRLVERHIGPRAEDIAAMLATLGFESLAEL
                     VAAVIPDSIRTREPLGIGPARGEQEVLAELRAIAGRNRVARSYIGMGYYGCHTPPVIQ
                     RNILENPAWYTQYTPYQSEIAQGRLEALLNFQTMVSDLTALPIANASLLDEATAAWEA
                     AVMCRAIANPGRGESTRNRFLVDRGCHPQTIALLKARADAVGMRIEVGAVDQMELAAD
                     VCGVLVQYPATDGAVEDYAPLTGRVHDAGALVACATDLLALTLLRPPGEWGADIAIGS
                     SQRFGVPMGYGGPHAAFLATREEFKRQLPGRLVGVSRDAAGKPALRLALQTREQHIRR
                     DRATSNICTAQVLLAVMASMYAIYHGPQGLKAIALRTHRFTAALAAGLRRLGHTIDPG
                     PVFDTLRVRLDPEAQARRLSRAAGAGINLRIWHDGSLGVSLDETVTLDDLSVLLWIFA
                     ADGEVPPDAAELVGAADDLIPDALARTSPFLTHPVFNTHHSEHEMLRYIHRLQSRDLS
                     LVHSMIPLGSCTMKLNATAEMLPITWPEIANLHPFCPSEQAEGYGVLVRQLEQWLASI
                     TGFAATSLQPNAGSQGEYAGLMAIRAWHASRGEGRRNVCLIPVSAHGTNPASAVMAGF
                     RVVPVACDDQGNIDLADLQARAEEHAERLGAIMITYPSTHGVFEESVREVCAVIHRHG
                     GQVYLDGANINAQVGLCRPGEYGADVCHLNLHKTFCIPHGGGGPGVGPICVAAHLAPF
                     LPGHPFTAGEGSAVGPVSAAPQGSAGILPIAWMYIALMGDEGLTRASEVALLNANYMA
                     NRLREHYPLLFTNRKGRVAHEFILDLRPLKKSAGIDPEDVAKRLIDYGFHAPTMSWPV
                     AGTLMIEPTESEPRAELDRFCDAMIAIRQEIREIEEGRADRENNLLKNAPHSIQAVAA
                     DEWPHPYSRERAVYPAPWLREAKFWPAVGRVDNAWGDRNVVCTCPPVEAWEG"
     gene            complement(9749..10003)
                     /locus_tag="PLS90_02590"
     CDS             complement(9749..10003)
                     /locus_tag="PLS90_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01322.1"
                     /translation="MEPGVPRTDIQRLYEDKIRQMPPHERVERATRMHELVVSILRQQ
                     LRAKHPELSEREISWKIAERMNWRKKRALELNRQVAEHEP"
     gene            complement(10006..11655)
                     /locus_tag="PLS90_02595"
     CDS             complement(10006..11655)
                     /locus_tag="PLS90_02595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPK01323.1"
                     /translation="MTKFIFITGGVISSLGKGIASASVGTLLKSHGYNITMMKLDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFTGVTMSRLNNVTTGQIYYSVISKE
                     RRGDYLGSTVQVIPHITDEIKGSILRLADQDSYDAILCEIGGTVGDIESQPFLEAIRQ
                     IPYDIGREHCMYIHLVLVPYLAAAGELKTKPSQHSVRALREIGIQADVLLCRTEHPLG
                     ESQRQKIALFCDVRPDMVFEARDADDIYRIPLLYAEQGIDRAIIKRLGLNRTEAQIDR
                     WRSLMQRVDSIPDTLRIGVAGKYVELQDAYKSIYEALRHGAIANNVHLEIDRIDSEQL
                     TAENVAGRLSDLAGILIPGGFGQRGIEGKLLAAQYARENGVPYFGICLGMQCALIEFA
                     RNVCKLEGANSREFDEDTPYPVVDLLATQKTITTMGGTMRLGAYTCRLHEESLAAQLY
                     GTTQISERHRHRYEVNNKYRALFEEHGLRLAGTTLDTKLVEMIEIPGHPFYVGCQFHP
                     EFKSRPLDPHPLFRGFIAAAKARAEAKGELVAAAQPSARGS"
     gene            complement(11751..12047)
                     /locus_tag="PLS90_02600"
     CDS             complement(11751..12047)
                     /locus_tag="PLS90_02600"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPK01324.1"
                     /translation="MLDFRRRLEALEVPLHRRVVYGSRARGDARPDSDDDVLLVLEND
                     NSQSRKSIHHLATEFGLEYDLSIFPVVMTRAQFEKEHVRYNLFMMNVDRDGVAI"
     gene            complement(12083..12835)
                     /gene="kdsB"
                     /locus_tag="PLS90_02605"
     CDS             complement(12083..12835)
                     /gene="kdsB"
                     /locus_tag="PLS90_02605"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPK01325.1"
                     /translation="MGPRIAGVIPARYGATRFPGKPLALLHGRPLIVHVCENVARARM
                     LERLWVATDDERIASAVEEAGFEARMTPSDCASGTDRIAASLRADEEWEILVNIQGDE
                     PRIDPQVIDSVAIALAEAKDCGVATAAVPIRNEADFRSPHVVKVVLTPEGRALYFSRS
                     PLPSPDRLTAAEREAPGFCWGLKHLGLYAFRRAVLSQFAAWLPTPLEQRERLEQLRFL
                     ENGVGIRVVEVEHDSIGVDTPEELALLQTRGA"
     gene            complement(12886..13761)
                     /gene="hisG"
                     /locus_tag="PLS90_02610"
     CDS             complement(12886..13761)
                     /gene="hisG"
                     /locus_tag="PLS90_02610"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPK01326.1"
                     /translation="MAPLIKLGLPKGSLQEATLQLFRKAGFYFSVSGRSYFVDSDDPE
                     IEAVLIRAQEMARYVASGAFDAGLTGHDWVMETGADVHEVCELIYAKVSARPVRWVLA
                     VPENSSIQSVKDLEGKRIATEVVGLTRRYLEQHGVQADVEFSWGATEIKAPQFVDAIV
                     EVTETGSSLRANKLRIVETLLESTTRFIANKQAWQDPQKREKLENMALLLQSAIAAEG
                     KVGLKMNVPKRNLEQVTRILPALTSPTISHLADSDWVAIEIITEEAEVKRIIPALRRA
                     GARGIIEYPLNKVID"
     gene            complement(13778..14962)
                     /locus_tag="PLS90_02615"
     CDS             complement(13778..14962)
                     /locus_tag="PLS90_02615"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPK01327.1"
                     /translation="MASIKTTFTTILILLILVALGVVGVFGYHWWQVRAVQGEIAQAR
                     TLLDTGSPQEAEQILATRMERSKPGQPWVAQIISMRFEAMEAYGDDAAAQRLAALVLS
                     EGQPWVRPPQEAWAQATLRLAQAELARNNIQGAQGYVEAVLAQPASQPVKDEAELAQA
                     RIELAQGKAEEAKARLEALLARVPEGSPVRRGVEHNLGLLNTRMLLSPQPFGEDELYV
                     VAKGDTIAGIAKRFGVSPDLIMRVNGIENPRMLSIGRRLKIPKLDLELVVNKTDNTLT
                     VYNGGKFFKRYDVRTGEYDYQTPPGEYRIQNKKENPAWTDPKTNEHYEPNAPGNQLGS
                     RWMGFSGSLGIHEAIDAESIGTYSSNGCVGLVKQDVEELYDLVRVGTPVRIVGQKSTP
                     SL"
     gene            complement(15687..15899)
                     /gene="rpsU"
                     /locus_tag="PLS90_02620"
     CDS             complement(15687..15899)
                     /gene="rpsU"
                     /locus_tag="PLS90_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPK01328.1"
                     /translation="MSLIFVDNNEPIDKALRRFKKECQKSGILAELRRREYYEKPSVR
                     RRRKHEAAKRKQRRRMLKMLRKMERY"
     gene            complement(16053..17333)
                     /locus_tag="PLS90_02625"
     CDS             complement(16053..17333)
                     /locus_tag="PLS90_02625"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPK01329.1"
                     /translation="MATRPTDIQKELDRRYEAALAAAAGQVTDQFDAPPEVVDAWRRA
                     IRVVRQALAELCWPQPTWGATLRQWRGHCRIQLGDLLPHPGIDTIEGLMSAMGLGRHL
                     RADYVEFTDLHSICRWLRKVYTALRQEQPPLKHPPDRVRLNIPGYSRNLADRWRAERR
                     LATLPRLIRRLRKGTPRSIDPILFGSLAREEAWPRFSDVDLCLIFDDDAITATSSLTR
                     TVRTCRQIAPLLAAADALHHHGPYVLFESDWDAYSESLLPLATLGRAMTMESRPYVQF
                     VRVYGDRHQGLSNLAQARRGILDLLGRPDKLADRYTAKYLVSLVLLLPALVCSARGIP
                     FDKADAFDFLKPLLNDPARQTLEAVEAIRREWRETIQPPAVLLRGCGALAGCVGRQVN
                     RWPSRSLQQRLIKLGGGIRHLADACLELGILELV"
     gene            complement(17394..18023)
                     /locus_tag="PLS90_02630"
     CDS             complement(17394..18023)
                     /locus_tag="PLS90_02630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPK01330.1"
                     /translation="MNWIVRPITGILRSLWRAYLRHETERIPFAARGVHVEIVPRSRF
                     ENPERIRIGNYVYIGHGAAFHGIGGITIGDNISFSPHVQIHTSNHRYEQADALPYDHV
                     SYLKPVRVESHAWIGANVLICPGVTIGEGAVVAMGSVVTRDVPPCTVVGGNPARVIKE
                     RDRARFEKLKAEGRFYMRIKHEPGYRGTVFVGPGVEPGIATDSSREAER"
     gene            complement(18020..18868)
                     /locus_tag="PLS90_02635"
     CDS             complement(18020..18868)
                     /locus_tag="PLS90_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPK01331.1"
                     /translation="MRELPFRLLDRLYMGLSRPFGRELRARALLDWRAAQSPARVEPP
                     EGSLLVLAPHPDDESIGCGGLILLKPDRAERVRIVLMTGGEQGRPDWTETATARTRRE
                     EMEACRRLLGVAQVHSLPGEDQRLSSQPELAAPLRELLDGLLPDAVLLPFFMDAHPDH
                     IATNRIFLEAAHDVLPEGLPVWGYEVWSHCPANVAVDISAVIDQKRAAIAVHASQDRR
                     MNYAEAIAGLNRYRAMQINWRGESDLTHAEAYVRMALREYRRAAEAWFSRTGVPAEPA
                     KSEGAP"
     gene            complement(18865..19740)
                     /locus_tag="PLS90_02640"
     CDS             complement(18865..19740)
                     /locus_tag="PLS90_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HPK01332.1"
                     /translation="MAEAPAQPVSSRPRVVILTSGLDNLPARLLLRECGRRSFRPAAV
                     VVDPIIRRRRAPEPSLLARYGRMLRARMGVYAARKLARGLRRRFERPDNPRLPWLDIL
                     AERQGVPLIQTTNVNRDEGLRRLRETAPEILVMMGCRILRPEALAVAPLALNFHTGLL
                     PDYRGADTLYWALARGDHERIGFTIHRAVEAVDAGEVYYEEAVAPLRGELIEDLFRRC
                     CERAAPHWFDLFAAFARDGKLEGRALDLTRGTRYWGVDWWAVAHAEHAWMKGRQPAWV
                     PENAPPAESAQGPNA"
     gene            complement(20008..20649)
                     /locus_tag="PLS90_02645"
     CDS             complement(20008..20649)
                     /locus_tag="PLS90_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01333.1"
                     /translation="MPAVVRGRSEGRSAAAGFEAARPRDGGATGGSARCAANERGLPA
                     GRTTRRGAGQRSSRTASSASGHSDRVGRRGEDGFAPATAFVLGSFFARVFFADDGLAS
                     GGAGRAAGAACLVVLDTLAAACLAGLSAAWALAALDRLVAGAFAVSVEGVFESFFAAF
                     RADFGAAASLLAALAGGFFFAAASSPAGREAGTESAVWSEGSGRFRAMLQPPR"
     gene            20774..21835
                     /locus_tag="PLS90_02650"
     CDS             20774..21835
                     /locus_tag="PLS90_02650"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HPK01334.1"
                     /translation="MPSASASQIVLMVRDPETLYIHWTLENEDLQRHGLDRRVGRPLL
                     MLRLAAADGPITGGFVQEWVVGIETRDHTLRIPRGERHWSVELGYVDRDGRFRTIARS
                     NRVISPHGQPAELPHEARVTRRAVPDWVTGQPRSPAPAPRHADVRLAAEDSRPTARPP
                     EGLPEWLAEIPPPQPTGPDPSTPTPAATTTADDTAHRPGAHPASQRVQGAQAKDVAPA
                     PVDALGAPLPQVAASQPRLGFAAPAGPSGAPRAEAPALDHVAQASGAGFHGAGQSAAL
                     PVDAFRLSVDADLTVYGATEPDARLTVQGRPITLRPDGTFTLRFSLPEGEQVIDVVAT
                     STDGRHTRTVTLIIRRATL"
     gene            22005..23249
                     /locus_tag="PLS90_02655"
     CDS             22005..23249
                     /locus_tag="PLS90_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK01335.1"
                     /translation="MNSRERVLAAIRHKEPDRVPVDFGAMRSTGITALAHVRLKRHLG
                     LAGPVQVYDLVQQLAQPEDEILDRFGIDAVDLGRAFLTDPAEWRPWTLPDDAPCLVPA
                     YFNPERQENGDWVARDADGDIVARMYHGAFYFNQEIHPLEDVPPDKWADSLPAAMAKV
                     SWAALPSPPYHIPLDDAGLARIAETAKAFHGQTDRAIMVAFGGNLLEWGQFLRGFGNL
                     LFDLMAEPAALERLLDRLVELHLENLRKILDAFGDAVDLIQFGDDLGTGAALQMSPEM
                     YRRFFKPRQKKLFGYVRERGGPHVFLHSCGSIREIIGDLIDAGVEVLNPVQTSAHGMD
                     PAELKREFGRDVTFWGGGCDTAHVLPRGTVQDVRRCVLERLEIFSKGGGYVFNQVHNV
                     TPEVPPENVVAMFEAVREFNGG"
     gene            complement(23273..23563)
                     /locus_tag="PLS90_02660"
     CDS             complement(23273..23563)
                     /locus_tag="PLS90_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HPK01336.1"
                     /translation="MPSWSNKDERMYEHIKESNRERGIKGKRAREIAARTVNKQRREE
                     GRTPNKTTQGTGNPRRSLDERSRDELYNIARDRKIRGRSRMSKGELIQAIRH"
     gene            complement(23786..24259)
                     /locus_tag="PLS90_02665"
     CDS             complement(23786..24259)
                     /locus_tag="PLS90_02665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPK01337.1"
                     /translation="MSEADSSAGSRPAPHISRYRVTYRMTDQMGVVYYGHYLELFEIG
                     RTEMLRDAGFEYRLLERDGYYLPVVHVSADYLSPARYDDLLLIHTWLEHLGKVRFDFR
                     YELCREGDDRALCRGRTRHVLVGADGRPRRLEGTWLERLKPFVAQSEDSGDSSPA"
     gene            complement(24421..24507)
                     /locus_tag="PLS90_02670"
     CDS             complement(24421..24507)
                     /locus_tag="PLS90_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HPK01338.1"
                     /translation="MGSVVKKRRKKIRKHKYRKLRKRMRHKR"
     gene            complement(24724..26169)
                     /gene="dnaB"
                     /locus_tag="PLS90_02675"
     CDS             complement(24724..26169)
                     /gene="dnaB"
                     /locus_tag="PLS90_02675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPK01339.1"
                     /translation="MSDDLLQDSPTLSLPHNLQVERALLGSLILDNRQIDLVLEIFHP
                     EAVSMARQGNAARRRGQNHLEPLFFSQAHQRVFGSICRLYDAGTGIDLTTLAEELNRQ
                     GDLELVGGAPYLASLEDDVFSLTQVPEYARIIMEKWKLRSLIRATQLISEVAASSDAR
                     ADEVIEQAEKRIFEIALDRVQRDFVQIGEPLAEQLIEIENRKKGVAELPGLPTGFRVL
                     DRMTTGLRPSQLIILAARPSMGKTALALNIAAHVAFRRGQTVGIFSLEMSASELITRL
                     MCTEARVSMGRVRGNFGLRRDELDAIHEAAERIEKAPLYIDDASSLSAMELRAKARRL
                     KSRCPELALIIVDYLQLMTGGGARYDNRQQEVSEISRSLKQIARELNIPVIALSQLSR
                     QSEQRQGRGVKDKLPRLSDLRESGAIEQDADIVLFIHRERNMATDEDGSHEPELATLR
                     IGKQRNGPIGDTQLLFRGEFTRFVDLALDSD"
     gene            complement(26378..26992)
                     /gene="rplI"
                     /locus_tag="PLS90_02680"
     CDS             complement(26378..26992)
                     /gene="rplI"
                     /locus_tag="PLS90_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPK01340.1"
                     /translation="MQVILFRSIDKLGMQGDVVNVANGYFRNYLGPRGLAVEASEATL
                     RRLEAKRKKLQLEAEKDRTDAESVAERLAGVRLHFTMKTIDGKKLFGSVTAQNILEQL
                     NEQGYAFERRQITLHEPLKTTGEHDVRIKLVGHIEGHIKVQIDAETTVSESEQTDMDL
                     AAAAQAASDRRRALREQQEAQESGEVAAPAVAEENAAEAEDGNS"
     gene            complement(27067..28005)
                     /locus_tag="PLS90_02685"
     CDS             complement(27067..28005)
                     /locus_tag="PLS90_02685"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPK01341.1"
                     /translation="MLLGAALAAVTVLAIVISAVGLAGGIVGGCLAAAAYWAAFDRAS
                     PPLVGLVLIGVVLATYYVLPELAVLWVMASALAGATGVWLNRAGGREDDYFFLPAAAT
                     LAAFAILVWLGQGGRLDGPLEALRESPTQLLNLIEQENARLIEQRQAPLFNPTQLEQL
                     REYLPRAWVPTLVALWVLALWLTGRVVRRAIGRFQPSRSLLVLFRLSQRYIFVLIAGL
                     FFAILGSLYAWPTAWRIALPLLAVMYVAALVQGIAVLLFTAALRRKMGQPGLAAAMTW
                     GGLILFIGFIRVPIGALLGLMDVWLDFRRLERRGAS"
     gene            complement(28139..28798)
                     /locus_tag="PLS90_02690"
     CDS             complement(28139..28798)
                     /locus_tag="PLS90_02690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPK01342.1"
                     /translation="MSFTTDSGQELAPGALARTNPTQLYLIRHGEPEACYLNCYYGQD
                     DVPLSERGRDQSRRLAERLAAVPFDAVYSSDLRRAGYLAELIAEGRGLPVRRLAVFRE
                     RHMGILQGLDVKLLETAHEHTEIYRQWRLDRVNFRVPEAENFVDLRDRVLPAIEELTQ
                     SFKGMRIALVCHAGPIRVALADALGMPLDNCFRFSLNHCSVSVIEYPHEGTPRVTLIN
                     G"
     gene            complement(28947..29732)
                     /locus_tag="PLS90_02695"
     CDS             complement(28947..29732)
                     /locus_tag="PLS90_02695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01343.1"
                     /translation="MPEPIRSICLTLPAYNEEAAIEPLLIRAEQALRPEPLEWSVIVV
                     DDGSRDATAAIVERLAAARPWLRLVRHDRNRGLGPAILTGIQSALEGPWAADRLIVCM
                     DADMTHPPETIPLMRARADATGADVVIASRYQPGSRQHGVPLFRRTLSFGARLLFRHY
                     LALPGVRDYTCGFRAMRASLLRRALARFGPGGLITRTGFACTDELLVHLAMVGARIEE
                     VPFILRYDLKQGASKMNLGLTIRETLKLLRSHRAQLRAGRDGE"
     gene            complement(29850..>30336)
                     /locus_tag="PLS90_02700"
     CDS             complement(29850..>30336)
                     /locus_tag="PLS90_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01344.1"
                     /translation="RITAKNRLTTETVITTSGPIWVFSTGVPDLAIEAGSLVAEPAFV
                     EQGDPFLVRWTETNPGSSTGDGHWSRVVASPDAEITGSGNDIVLGEAWTNPIGPGTSV
                     PQQIQADSSGLDLGSYTLAVLVDFYDHEKDESNENNIFTADKPLVIIEQNAVGNWHLF
                     Q"
BASE COUNT         5126 a        10103 c         9983 g         5124 t
ORIGIN      
        1 gcgctgggac ccctttcaga atgccggcgt caagcaggtg tgggtccacc tgatgaacaa
       61 cttcaacgac cgcgggacgt cgaacgaagg ccgactgctg gcggtgccgg tcaacatccg
      121 gacgaaggcc aggctgcagg tcggccgcgt gtgggccgag gcgacccccg acgagatcga
      181 cgacaaccgg atgcgcctgc tggccgaagt gaaaaacacg ggcgaaacgg ccgcccgcgg
      241 cgtcgtcgta agcttctacc gcagcgcgat ccagaccgag gaaaacgaac tgggcgaggt
      301 gatgctgaac cagatcgccg ggggcacgag cgggatcgcg acgctcctct gggagttcga
      361 tccgcggcgc gacctggtgc gcggcacaca gttgccctcg ccgaccgtgc gcgtgtggct
      421 caagggctcc agccagcgga tcagcagcgt gcccgatgca acggacgacg gatcgagccc
      481 gctggaggaa tagcggcgga aaaatcaaat cggggcgaag tcgcggtcgg cttcgccccg
      541 gcttgccttg tctcggtggg gggggttact tgggcgcgtt ctggaacttg gagtagcggc
      601 ggttgaattt ctcgacgcgg ccggccgtat cgacgagctt ctggcggccg gtgaagaacg
      661 gatggcagga agcgcagatc tccagccgct ggttctcgcg cgtggagcgg actttgtatt
      721 cggcgccgca ggcgcagcga atcgtcgtca gtttgtactc ggggtgaatg ccctttttca
      781 tcattggtcc tttgctggtc cgattggcgc ggggtctccc gggagcctct gaggccaaga
      841 acgggtttcc ggcgctcagc acaaaccacc atttaatagc caacctaccc ccgcctgtca
      901 aggatctgcg ttgtctccac tgcttccgtg gcggttttgg gccggcgcgg aagagaggca
      961 acgggcatcc ggaccggaac ggaagagggg cttgcacggt tgcgggcata gccgccgcga
     1021 gagcatcgca aaggacattt tcgcttcctt ccgcaccgcc tcgccgactc tccccgccgg
     1081 acggggtttc acaaacctga cgtcaaaccg gcgccggcgc ggtttcgatg acgcgcatcg
     1141 tgacccagtg gtttttcagg aaccggcgca gcatcaccag gccggcgacg ttgacgaacg
     1201 tcgtggcgaa gatccaggcc caatacacgc ccccccagcc ccgttcaata aacagccagg
     1261 tgggaatgac catgatcgtc cacgccagcg tgaggtggac caggagcacg aagcgcgtgt
     1321 cgcccgcccc cttcagcgcg gaggagaaga tgatgaccat cacatcgaac aggcagtagg
     1381 cggcgacgaa ccgcaacagc accaccgcca cgcgactgac ctgaaggaac tcggccgccg
     1441 gcgcctcgcc cgcgaaccag cgaatgaaca cgttgggaat gagcacgaac agcagggcgc
     1501 acagccccat gtagcagaat gcgacgtgga acgccgccca ggtggcgcgc gccgcgcgtt
     1561 cggggtgatt ctcgcccagg tgctggccga cgagcgtcgt gacggcaatc cccaggccga
     1621 tcatcggcat gaacgcgatc atgttgatct gaaaggcgag gttggtggcg gtcagttcgg
     1681 tcatgccgag ccggccgacg aggagcgtga acgcggcgaa actgaggatg tcgatcataa
     1741 tctggccgcc gctgggtgcg ccgaagcgca gcagtcgctt gaggagcgcc cactccaggc
     1801 gccagcgccg gccggtggcg tagcgccgcc ggttgtaggc gttcaggaac aggacgagat
     1861 agacggcgga gttgaccgcc gcggagacga acgtcgccca ggccgcgccg gcaatgcccc
     1921 attcgggaaa gccccagtga ccgaatatca tcgcgtagtc cagcacgatg ttgaccagcg
     1981 tgctggcgac gttgacgagc aggacggtcc aggtgcgtcc caggccggtg tacagggccg
     2041 agacggcgct cgacagcgtg atgaatcccc ccgtgtagca ggtgattctg aaataggtga
     2101 gttcgtgggc caggacatca ggcggatgat cggcccagac caggacgcgg ctggtcagcg
     2161 ggatggccgc aatgatgatg acgccggcgg cgatcgagaa gtagaccgcc tgccagacgg
     2221 ccgcgcccac ccggtcgggc cggcccgcgc cgttgtactg cgcgacgaag gcgttggcgt
     2281 agcccgccgt ccccatgaag aaaccgatga tgacgaagga aaaccagctt gcggggaggg
     2341 acgccgcgaa ctcggcttcg ctgtgccagg agaggaacag gcgatcgatg aactgctgga
     2401 tcgtcagcga actggtgctg aggatcagcg gcccggcgat ccagaagata tcgcgatagc
     2461 ccccccgctc acgccagcga acccggagat cgtcgaggtt gagcgggggc aggctggcgt
     2521 gatgcatggc ggccgcgggg gaactggatg cgcgtcggcc ccgggggcgg acgcgggctc
     2581 atccatcgta gcccgccggc gcggatgccg caatcaccgg tcgcgtgcca ggtcttatgc
     2641 cccggccgac ccttccatcg tctccgcgcc ttccgccggt tccgtcgctt ccgtcccttc
     2701 ctcgtcaggc cgcagttgct tcaggtactt ggcggggttt ttctccagtt cggcgacgca
     2761 cgccgggcag cacagccgga tcagttgcgt gccgagaacg taatccaccg gttcaccgtg
     2821 atcgcccagc gtttcgccgc tgacgatgca agtggcgagg ggataatcgg cccgctgcgc
     2881 ttcgatgacc gcctgatcga tctgctcgaa gtactgagcc ggatcacttt gaaattcagc
     2941 gacgcaggcc cggcagcaga accgcaccag gcggttgtcg tgcacatagt cgaacggttc
     3001 gcccatgccg cccagcttct cgccgctgac gacgcacgtc tccagcggat aggtcggctt
     3061 ctgttgctcg atgagctgct cgttgatctt gatcagaaac gacgtcggat cctccttgaa
     3121 cggcgcctcg cacgaggggc agcagaatcg gatctcgcgc ccttcgtatt cgatcaccac
     3181 cggatcgccc atcgcgccga gcggctcgcc ggagacaggg caggtggtca gcgggtacgg
     3241 gttgcctttt ttcgccttgg gggatgattc cgttggcgcg gcggcggctt cgggcgttgc
     3301 gggctgggaa tcctcggcca cggcgacgcc ggcgggaatc gcggtagaga gcaacagcca
     3361 cagcgcggcc aggagggcaa gcggtcggtt catgggaaac atccttggtt gaattggggc
     3421 gagggcatgt ggacgcgatg ccccctctcc cctcacgcat gcagcgcacc agaacggccg
     3481 ccgaagccgc ccgccccgag caaagcttgg gaagggcatc gacgcttgaa gtgcaacggc
     3541 ttagctcgcc attctgagat cattgctttg gcgcggttct ggattcgtgc cgcgcaattc
     3601 ccagcccggt gatgcagatg ccacaaatgt gatgtcggtt ttcagtcagg cgcctcccgt
     3661 gcgatctctg ggaggcgcga cttcatttac ggatgcggcg ccggatctta aggaaagatc
     3721 tcgctcacgt gctccgaaaa attgacaagc tcctgtcgag tggtagaatg gacgcctcac
     3781 gagcatggct ggtcgccttc cgaaaggagt cagatccaga gaattcagca catctggtgg
     3841 atgttcgcgg gagagtgctc ccttgccgga gccgccgaag aagcaaaacc gcccaggaaa
     3901 ctttcaggct caaggaccgc gaacggacag aactctggag agcggatcgc atggcgccgg
     3961 cagccggcaa gccgggcgat cccaccgacg gggaggccgc gcggccgcga gacggccggc
     4021 ggctgaatct ctcaggtaag cggacagagg ggcggaaacc ggaacacgtt tagctaacgg
     4081 tgttccgatg tgtttccgca tcccgcccgt tccacttgag aggcccatgg acgcgcaggt
     4141 ttcattcagt cccaactccc atcactcgcc catggaatcc gcacgcctgc cgcactggtt
     4201 gaagcggccg ctgggcggcg gaccgctcta cgcccgcacg cgcggggccg tcgattcagg
     4261 ccgactccat acgatctgcg aggaagcgcg ctgtcccaat cggggcgagt gctggagccg
     4321 cggcacggcg acgtttctga tcctcggcga tcgctgcacg cgccgctgcg gcttctgcag
     4381 cgtcaagccg ggccggccgc gcgggcgcgt cgatggcgac gaaccgcgcc ggctggcgga
     4441 agcggtcgcg cacatgcggc tcggctatgt ggtgctcacg tcggtcaatc gcgatgatct
     4501 gcccgacggc ggcgccgcgc atttccgcga ctgcatccgc gcgatccgcg ccgccgttcc
     4561 cggcattcgc atcgaactgc tcacgcccga cttccgacgt tgcgccggcg aagccctcgc
     4621 cacgctggcc tccgaggccc cgctggtctg ggggcacaac gtggagacga cgccgcggct
     4681 ctacgccgct gtccggcccg gctccaatta tcaacactcg ctggatctcc tgcgccgcgt
     4741 cgccctcctg ccgggcgtgg cggcgaaaag ctcgctgatg ctcggcctgg gcgaacggga
     4801 agaggaggtg ctgcgcgtgc tcgacgatct ggcgcgggtc gggatcgaac ggctgacact
     4861 ggggcagtat ctgcggccga cgcccgcgca gttgccggtg gtcgaataca ttcaccccga
     4921 ccggttccac cggctggccg acgaagcccg ccggcgcggc atccggtggg tgatggcgtc
     4981 gccgttcaca cgcagctcat atttcgccga ggccgagcgg cccggcggga aagcggggat
     5041 ccacgcatga ccacgccgag tctcctccgc acgcccctgc acgccgcgca catcgcggcc
     5101 ggggcccgca tggcgccctt cgccggttgg gaaatgccgc tgcactacgg ctcgcagatc
     5161 gacgagcatc acgccacgcg caaggcctgc ggcctgtttg atgtctcgca tatgggccag
     5221 gtgcgcatca ccggttgcga cgcgctgaag ctggtgcagg cgatcgtcac gcgcgacgtg
     5281 gcccatctgg gcgacgggcg atcggcctac tgcgcgatgt gcaaccgcgc gggcgggctg
     5341 ctggacgatc tgatcgtctc gcggctggcg atgggcgatt acctgcttgt ggtcaacgcc
     5401 gggacgtatg agggggacct ggcgcagatg cacgcggtcg agcgcgaatt cggcccgttc
     5461 gatgcccgga tcgacggctg tcgcgatgac tgggcgatgc tggccgttca gggccccgaa
     5521 gcccccgcgg tgctggcgcg cgtgcttggc ggcggcctcg acccgcccgc gatgcggatg
     5581 atttccgtcg aatggcgcgg gcaggccttc tggctctcaa cgacgggcta caccggcgag
     5641 cgtggcgccg aactgatcgc cccggtcgag atggcgcgcg acctgtggga cgctctgatg
     5701 gcggccggcg cgcgacccgt cgggctggcc gcgcgcgaca cgctgcgcct ggaacaggca
     5761 ttcaatctgt cgggacagga ctttaccgcg caaaaccatc cgttcgaggt gcggctgggt
     5821 tgggtcgttg atctggacaa acccgacttc tgcgggcggg aggccttgcg cgcgatccgc
     5881 gccggcgtct gtccgcggca gcagtgcggc ctgctgcccg agggccggag aatcccgcgt
     5941 catggcgcga gggtcctgca ccaggggaaa caggtcggcg tcgtcaccag cggcggcttc
     6001 tccccgacgc tcgaccgccc gatcgcgctg ggcctgctcc agagtcagat ggtcaaatcg
     6061 gaagaaccgc tggaaatcga tctgggtcgg ggcgcgacgg tcccggccgt catcacgccg
     6121 ctgcccttcc tgcgggcgca acccctgtga aaggacccgt tcatgcagcc cgagacgctg
     6181 agataccgcg caacgcacga gtggattgaa gcctccggcg ccgagcgccg cgtcggcatc
     6241 agctcgttcg ctcaggaaca actgggcgac atcgtctttg tggaactgcc gaccgacctg
     6301 ccgcgcgacg tggcggcggg ggacgaagtg ctggtgatcg agtcctgcaa ggccacctcg
     6361 tcggtctacg cgccgctggc cggccgcgtc accggcgtca atcaacggct ggccgaccat
     6421 cccgagcgcg tcaatcagga cccgaccggc gaaggctggc tgttcacgct ggaggtcgcg
     6481 cccggcgccg acgaatccga actgctcgat gccgaaggct accgccgcct ttgcgaggcg
     6541 gaagggtagc gattcgctcc ggtcgcattg ttcccgctgc atcccccgaa ggagttcgtt
     6601 catgacggtc gccccgcttc cctccgtatc gctgcagccg ctggatcggc tggtggaacg
     6661 ccatatcgga ccgcgcgcgg aggatatcgc cgcgatgctg gcgaccctcg gcttcgagtc
     6721 gctggcggaa ctggtggcgg ccgtcatccc cgattcgatc cgcacgcgcg agccgctggg
     6781 gattgggccg gcgcgcggcg aacaggaggt gctggccgaa ctgcgcgcga tcgccggccg
     6841 caaccgcgtc gctcgctcgt atatcggcat ggggtactac ggctgccata cgccgccggt
     6901 gatccagcgc aacatcctcg aaaacccggc gtggtatacg caatacacgc cgtatcaatc
     6961 ggagatcgcg caggggcggc tggaggcgct gctcaacttc cagacgatgg tgagcgatct
     7021 gaccgcgctg cccatcgcga acgcctcgct gctggatgaa gcgacggcgg cgtgggaggc
     7081 ggcggtgatg tgccgcgcga tcgccaaccc cgggcgcggg gagagtaccc gcaaccggtt
     7141 cctggtcgat cgcggatgtc atccgcaaac gatcgcgctg ctgaaggccc gcgccgacgc
     7201 cgtcggcatg cgcatcgagg tgggcgcggt cgaccagatg gaactggcgg cggatgtctg
     7261 cggcgtgctg gtgcaatacc ccgcgaccga tggcgccgtc gaggattacg cgccgctgac
     7321 ggggcgggtt cacgacgccg gcgcgttggt ggcgtgcgcg accgacctgc tcgcgttgac
     7381 gctgctgcgt ccgccgggcg agtggggggc cgacatcgcc atcggctcgt cgcaacgttt
     7441 cggcgtgccg atgggctatg gcggcccgca tgcggcgttt ctggcgacgc gcgaggagtt
     7501 caagcgccag ttgccggggc ggctggtcgg cgtgtcgcgc gatgcagccg gcaaaccggc
     7561 gctgcggctg gcgctccaga cgcgcgagca gcatatccgc cgcgatcggg ccacctccaa
     7621 catctgcacc gcccaggtgc tgctggcggt gatggcgtcg atgtatgcga tctatcacgg
     7681 ccctcagggg ctgaaggcca ttgcgctgcg cacgcatcgg tttaccgccg cgctggccgc
     7741 gggcctgcgt cggctgggcc acacgattga tccgggcccc gtgttcgata cgctgcgggt
     7801 gcggctggat cctgaggcac aggcgcgccg actgagccgc gccgccggcg ccggcatcaa
     7861 tttgcgcatc tggcacgatg ggtcgctcgg ggtttcgctg gatgaaacgg tgacgctcga
     7921 cgacctgtcg gttctgctgt ggattttcgc cgccgacggg gaggtgccgc ccgacgccgc
     7981 cgaattggtc ggcgccgccg acgacctgat ccccgacgcg ctcgcgcgca cgtcgccctt
     8041 cctcacccat ccggtgttca acacgcatca ctccgagcac gagatgctgc gctacattca
     8101 ccgcctgcag tcgcgcgatc tttcgctcgt ccattcgatg atcccgctcg gctcgtgcac
     8161 catgaaactc aacgcgacgg cggagatgct gccgatcacg tggcccgaaa tcgcgaatct
     8221 gcacccgttc tgcccgtcgg aacaggccga gggctacggc gtcctcgtcc ggcaactcga
     8281 gcagtggctt gcctccatca cgggtttcgc ggcgacctcg ctgcaaccca acgccggctc
     8341 gcagggcgaa tacgccggat tgatggcgat tcgcgcgtgg catgcctcgc gcggcgaggg
     8401 gcgccgcaat gtctgcctga tcccggtctc ggcgcacggc accaaccccg ccagcgcggt
     8461 gatggcgggc ttccgggtgg tgccggtcgc ctgcgacgat cagggcaaca ttgacctggc
     8521 tgatctacag gcgcgggccg aggaacatgc cgagcggctc ggcgcgatca tgatcaccta
     8581 cccctcgacc catggggtct tcgaggagag cgtgcgcgag gtgtgcgctg tgattcaccg
     8641 tcacggcggt caggtttacc tggatggcgc gaacatcaat gcgcaggtgg gactctgccg
     8701 gccgggcgag tatggcgccg acgtgtgcca tctgaacctg cacaagacgt tctgcattcc
     8761 gcacggcggc ggcggtcccg gcgtgggacc gatctgcgtc gcggcgcatc tggccccctt
     8821 cctgcccggc catcccttca ccgcgggcga ggggagcgcc gtcggccccg tcagcgcggc
     8881 gccgcagggc agcgccggca tcttgccgat cgcgtggatg tatatcgcgc tgatgggcga
     8941 tgaggggttg acgcgcgcca gcgaagtggc gctcctcaac gccaactaca tggccaaccg
     9001 cctgcgcgag cactatccat tgctgtttac gaaccgcaag gggcgcgtcg cgcacgagtt
     9061 catcctcgat ctgcgtccgc tgaagaagtc agcgggcatc gacccggaag acgtggccaa
     9121 acggctgatc gactatggct ttcacgcgcc gacgatgtcg tggccggtgg ccggcacgct
     9181 gatgatcgaa ccgaccgaga gcgaaccgcg ggcggaactg gaccggttct gcgacgcgat
     9241 gattgcgatc cggcaagaga tccgcgaaat cgaggagggg cgggccgacc gcgagaacaa
     9301 tctcctgaag aacgcgccgc actcgatcca ggccgtcgcc gccgatgagt ggccgcaccc
     9361 ttacagtcgt gagcgggcgg tgtatcccgc gccgtggctg cgcgaggcga agttctggcc
     9421 ggccgtcggc cgcgtcgaca acgcctgggg cgaccgcaac gtcgtctgca cctgcccgcc
     9481 ggtggaagcg tgggaaggat gatattctcg agatcgaagg cctgaaacat gctcccctgg
     9541 gcaaagcctt ccttgaaact gcggagcgag atctccgtgc agaaggaaaa tcttgggacc
     9601 gggcaaccgc ggaacaacca cgtcgatgtc ctgcgtggag cgtggttcgc cgtaaaatgc
     9661 cgagacgata ccgcccctca gatgataggg aatctcggat ttctcgacta cggggatcag
     9721 gcgccgaagc atctattcgt tgtcgggctc atggctcatg ttcagccact tgacggttca
     9781 actccagcgc gcgtttttta cgccagttca tccgttccgc aatcttccac gaaatctccc
     9841 gctccgaaag ttcaggatgt ttcgcgcgca gttgctgccg gaggatactc acgaccagtt
     9901 catgcatccg cgttgctcgc tccactcgct cgtgcggcgg catctggcgg atcttgtctt
     9961 cgtagagccg ctgtatatcg gtgcgcggga cgccgggttc catgatcagc ttcctctcgc
    10021 gctcggttgc gccgccgcca ccagttcccc tttcgcctcc gctcgcgcct tggccgcggc
    10081 gatgaatccg cggaacagcg ggtgcgggtc cagcggccgc gacttgaatt cgggatggaa
    10141 ctggcagccg acgtaaaacg gatggccggg gatctcgatc atctcgacga gcttggtgtc
    10201 gagcgttgtc ccggccagcc gcagcccatg ctcctcgaat agcgcgcggt acttgttgtt
    10261 gacctcgtac cgatgacggt ggcgctcgga gatctgcgtg gtgccgtaga gctgcgccgc
    10321 caggctctcc tcgtgcagcc ggcaggtgta ggcgcccagc cgcatcgtgc cgcccattgt
    10381 cgtgatcgtc ttctgggtcg cgagcagatc gaccacgggg tagggggtgt cctcgtcgaa
    10441 ttcgcggctg ttggccccct ccagcttgca gacgttgcgg gcgaactcga tcagcgcgca
    10501 ctgcatgccc aggcagatgc cgaagtaggg gacgccgttc tcgcgggcat actgggcggc
    10561 gagcagcttg ccctcgatcc cgcgctggcc gaatccgccg gggatcagga tgccggcgag
    10621 gtcgctcagg cgcccggcga cgttctcggc cgtcagttgc tcgctgtcga tccgatcgat
    10681 ctccagatgg acgttgttgg cgatcgcgcc atgacgcagc gcctcgtaga tgctcttgta
    10741 ggcgtcctgc aattcgacgt atttgccggc cacgccgatg cgcagcgtgt cggggatgct
    10801 gtcgacgcgc tgcatcagac tgcgccagcg gtcgatctgg gcctcggtcc ggttcaggcc
    10861 cagccgcttg atgatggcgc ggtcgatccc ctgctcggcg tagagcagcg ggatgcggta
    10921 gatgtcgtcg gcgtcgcgtg cctcaaacac catgtcgggc cggacgtcgc agaacagggc
    10981 gatcttctgg cgctggctct cccccagcgg gtgctcggtg cggcagagca ggacgtcggc
    11041 ctggatgccg atttcgcgca gggcgcgcac gctgtgctgg ctcggtttgg ttttcagttc
    11101 ccccgccgcg gcgaggtacg gcaccagcac caggtgaata tacatgcagt gctcgcggcc
    11161 gatgtcgtag ggaatctggc ggatcgcttc gaggaacggc tgggactcga tgtcgccgac
    11221 ggtgccgccg atctcgcaca ggatcgcgtc atagctgtcc tggtcggcca gccggaggat
    11281 cgagcccttg atctcgtcgg tgatgtgcgg gatgacctgg acggtggagc cgaggtagtc
    11341 gccgcgccgt tccttgctga tcaccgagta gtagatctgg ccggtggtga cgttgttcag
    11401 ccggctcatc gtgacgccgg tgaaccgctc gtagtgcccc aggtcgaggt cggtttccgc
    11461 gccgtcgtcg gtcacgaaga cctccccgtg ctgaaacggg ctcatcgtgc cggggtcgac
    11521 attcaagtaa gggtcaagct tcatcatcgt aatattatag ccgtgagact tcagcaacgt
    11581 gccgaccgac gccgaggcga tgcccttgcc gagcgaactg atcacgccgc cggtgatgaa
    11641 gatgaacttg gtcatgaacg ggtctcctta tgcggtatcg ggagagggga gtggagtaag
    11701 cggatcgagc gaatagccct gggcgggttg gcaatcggac ggctccaaag tcatattgcc
    11761 accccatccc ggtccacgtt catcatgaac aggttgtagc gcacgtgctc cttttcgaac
    11821 tgggcgcggg tcatgacaac cgggaagatc gacagatcgt attccaatcc gaattcggtc
    11881 gccaggtgat ggattgactt gcgcgactga ctgttatcgt tctccagcac cagcagcacg
    11941 tcatcgtcgg aatcgggacg agcgtcgccg cgcgcgcgcg atccatacac caccctccga
    12001 tgcaaaggga cttccagcgc ctccaaccga cggcggaaat ccagcatgat ttgatggtct
    12061 tgatgagaaa gcatcgccgc cctcatgcgc ctctggtctg caggagggcc agttcctcgg
    12121 gtgtgtcgac cccgatcgag tcgtgttcga cctccacgac gcggatgccg acgccattct
    12181 ccagaaaccg cagttgctca agccgctcgc gctgttcgag cggcgtcggc aaccaggcgg
    12241 cgaactggct cagaaccgcg cgccggaacg catacaagcc cagatgtttc agcccccaac
    12301 agaagcctgg ggcttcgcgc tcggcggccg tcagccggtc gggcgagggg agcggcgagc
    12361 gggaaaaata cagcgcgcgc ccctcgggcg tcagcacgac cttgaccacg tgcggcgacc
    12421 ggaaatcggc ttcgttccgg atcgggacgg ccgccgtcgc gacgccgcaa tcctttgctt
    12481 ccgccagcgc aatcgccacg gaatcaatca cctgcggatc gatccgcggt tcgtccccct
    12541 ggatgttgac aagaatctcc cattcctcgt cggctcggag cgaagcggcg atccggtccg
    12601 tccccgacgc acagtcgctc ggggtcatcc gggcctcgaa cccggcctct tcgaccgcgg
    12661 aggcaattct ttcatcatcg gtcgcaaccc aaagccgctc aagcatccgg gcgcgcgcga
    12721 cgttctcgca gacgtggacg atcaggggcc ggccgtgcag cagcgccaac ggtttccccg
    12781 ggaaacgggt ggcgccgtaa cgggcgggta tgactccagc gattctcggt cccatctgca
    12841 agcgtccttc acgccgccct gcgttctgct gagccgcaaa gccttctaat caatgacttt
    12901 attcagcgga tattcgataa tcccgcgagc gccggcccgg cgcagtgccg ggatgatccg
    12961 tttcacctcg gcctcctcgg taatgatttc gatcgccacc cagtccgaat cggccagatg
    13021 cgaaatcgtc ggcgaggtaa gcgccggcag aatccgggtc acctgctcca gattgcgctt
    13081 ggggacgttc atcttcaggc ccactttccc ttccgccgcg atggccgatt gaagcagtaa
    13141 ggccatgttt tccagctttt cgcgtttctg gggatcctgc caggcctgct tattggcaat
    13201 aaaccgcgtg gtgctttcca ggagggtctc gacgatgcgc agcttgttcg cccgcagcga
    13261 actgccggtt tccgtcactt cgacgatcgc atcgacgaac tgcggcgcct tgatttcggt
    13321 ggccccccaa ctgaactcca cgtccgcctg gacgccgtgc tgttccagat accggcgcgt
    13381 gagcccgacg acctcggtgg cgatgcgctt gccctccaga tccttgaccg actggattga
    13441 gctgttttcc ggcacggcca gaacccagcg gaccggccgc gccgagacct tggcgtagat
    13501 cagctcgcac acctcgtgaa cgtcggcgcc ggtctccata acccagtcgt ggccggtcag
    13561 gccggcgtcg aaggcgcccg acgcgacgta gcgcgccatc tcctgggcgc ggatcaacac
    13621 ggcttcgatt tcggggtcgt cgctatcgac aaagtaggag cggccggaga ccgaaaaata
    13681 gaacccggcc ttgcggaaga gctgcaaggt tgcctcctgc aggctgccct tgggcaaacc
    13741 cagcttgatc aggggggcca tgacgcggta ctgcctttca cagagatggg gtcgatttct
    13801 ggccgacaat ccggaccggc gtgccgacgc ggaccagatc atacagctcc tcaacatcct
    13861 gcttcaccag cccgacgcag ccgttcgagg aataggtgcc gatggactcc gcatcgatcg
    13921 cctcgtggat tcccaggctg cccgaaaagc ccatccaacg cgatcccaac tggttgcccg
    13981 gcgcgttggg ttcatagtgc tcgttcgtct tgggatcggt ccaggcgggg ttctccttct
    14041 tgttctgaat gcggtactcc ccgggcggcg tctgatagtc gtattcgccc gtgcggacat
    14101 cgtagcgctt gaagaacttc cctccgttgt aaacggtcag cgtgttgtcg gtcttgttga
    14161 cgaccagttc caggtcgagt ttgggaattt tcaggcgccg gccgatgctg agcatgcgcg
    14221 ggttttcaat cccattgacg cgcatgatca ggtcagggga gacgccgaac cgcttggcga
    14281 tgccggcgat cgtgtcgcct ttcgcgacca catacagctc gtcctcaccg aagggctgcg
    14341 gcgagaggag catgcgcgta ttgagcaggc cgaggttgtg ttcaaccccc cgacggacgg
    14401 gcgagccctc cggcacccgc gccagcagcg cctccagccg ggccttggct tcctcggcct
    14461 tgccttgggc cagctcgatc cgcgcctggg ccagttccgc ctcatccttc accggctggc
    14521 tggccggttg ggccagaacg gcttcgacat agccctgggc gccttggatg ttgttccgcg
    14581 ccagctccgc ctgcgccaga cggagcgtcg cctgcgccca ggcttcctgc ggcggtcgga
    14641 cccaaggttg gccttccgac aggaccaggg ccgccagccg ctgagcggcg gcgtcatcgc
    14701 cgtaggcttc catcgcctcg aatcgcatgg agatgatctg cgccacccac ggctggcccg
    14761 gtttgctccg ttccatccgg gtcgccagaa tctgttcggc ttcctggggc gagccggtgt
    14821 cgagcagcgt gcgcgcttgc gcaatttccc cttggacggc tctcacctgc caccagtggt
    14881 agccgaaaac gccgacgacg cccagggcga ccaggatcag gaggatcagg atcgtggtga
    14941 aggtggtctt gatggaagcc atggctggct cgctttgggg ggccgcgcgg ggaaaaacgg
    15001 gcccaaaata aaaaacccgt cgccaggaag acctggtcgg tcccccagcc acgggattta
    15061 agcctagcgg gggacgaggg ggattgtcaa gccgagggga cgggggtttt tgcggcccct
    15121 cgggccgtta ttatccgtca tggaaacccc ctttcgcccc gaaccttcac atgcggctgg
    15181 cagccgtaaa caatgtagaa tgaggacgaa acggatcaga tcgcacaatc gatccataac
    15241 ttcgttaaat taccatttaa cgaacatcga cttgggtcga aaaaaagagg gctgccagcc
    15301 ttctcttggg cggcctatga accggcggtt cagtccgttc ggaaaatagg gcggctgccg
    15361 actcccgagg acgagggacg gtggacgaga gccgaaatct cagggaactc cctgggctcg
    15421 acggcgagga tgagggacga gcgacgtttg aggatgaggg acgagcgacg ttttcagagc
    15481 gagggcaggc gtcgtgcgac gggcttttcg gaattccatc ccttccgcgc gccgctcacc
    15541 tcgccgcgcg gagcactctc ccggccggcc gctccgccga cgggagcgcg ccgggcggat
    15601 cgccggcccg gcccaagcaa aatcccccgg ccgatccgag gaccaaccgg gggacgatcg
    15661 aaagaactcg ttcgcctgga agggggctag tagcgttcca tcttgcgcag catcttcaac
    15721 atgcgacgcc gctgtttgcg cttggcggct tcgtgtttgc ggcggcggcg gacgctgggc
    15781 ttttcatagt actcgcgtcg gcgcagttcc gccagaatgc ccgatttctg gcactccttc
    15841 ttgaaacgtc gaagcgcctt gtcaatcggc tcgttgttgt cgacgaagat caggctcaag
    15901 acggattcac ctccctccgg gtcgaaaatg gatgctccgg gcggccggcg gcgtatcggt
    15961 cgcttccgcc ggacccgaac gcaacattct gcgcggattg gcgttttgtg tcaatctgga
    16021 tcgatcaagt ttccgccatc ctggcgcggc gatcagacca actccaggat gcccagttcc
    16081 aggcacgcat cggccagatg gcggatcccg ccgcccagtt tgatcagccg ctgctggagc
    16141 gaccgggaag gccaacggtt gacctgccgg cccacacaac cggccagcgc tccgcatccc
    16201 cgcagcagca cggccggcgg ctgaatcgtc tcgcgccact cgcgccggat cgcttcgacg
    16261 gcctcgagcg tctgccgggc cggatcgttc agcagcggct tgaggaagtc gaacgcatca
    16321 gccttatcga acgggatacc gcgggccgaa cagaccagcg ccggcagcag cagaaccagc
    16381 gagacgagat atttggccgt gtaacgatcg gccagtttgt ccggtcggcc gagaaggtcc
    16441 aggatccccc gccgcgcctg cgccagattg gatagcccct gatgccggtc cccgtagacg
    16501 cggacgaact gaacgtaagg gcggctctcc atggtcattg cccgacccag cgtggccagg
    16561 ggaagcagcg actcgctgta ggcgtcccag tcgctttcga acagaacata ggggccatga
    16621 tgatgaagcg catcggccgc ggccagcagc ggggcgatct gacggcaggt tctgacggtc
    16681 cgcgtcaggc tgctcgtggc cgtgatcgcg tcatcatcga agatcaggca gagatcgaca
    16741 tcggagaacc gcggccaggc ttcctcgcgc gccaggctgc cgaacaggat cgggtcgata
    16801 ctgcgcggcg ttcccttgcg cagcctccga atcaggcgcg gcagcgtggc caagcgccgt
    16861 tcggcccgcc agcgatcagc cagattccgc gaatagccgg gaatgttcaa ccgcacgcgg
    16921 tcgggagggt gcttcagcgg cggctgctcc tgtcgcaacg ccgtatagac tttgcgcagc
    16981 cagcggcaga tggagtgcag atccgtgaac tcgacataat ctgcccgcag gtgccggccc
    17041 agtcccatgg cgctcatcag cccttcgatc gtgtcgatcc cagggtgggg cagcaagtcg
    17101 cccagttgga ttcggcagtg cccgcgccat tggcgcaggg tcgcgcccca ggtcggttgc
    17161 ggccagcaca gctcggccag cgcctggcgc acgacccgta tcgcccggcg ccaggcgtcg
    17221 acgacctccg gcggcgcatc aaactggtcg gtcacctgcc cggcggcggc ggccagggcg
    17281 gcctcatagc ggcgatccag ttctttctga atgtccgttg gacgcgtcgc cattcagtcg
    17341 tggtcctcca tagagatttc cgccctttcg tccgtcggcg gcgccggacc ggtctaccgc
    17401 tcggcctcgc ggctggagtc tgtggcgatc ccgggctcca ctcccggtcc cacgaagacg
    17461 gtgccccggt agcccggttc atgcttgatg cgcatgtaga accgcccctc ggccttcaac
    17521 ttctcgaacc gcgcccggtc gcgctccttg attacccggg ccggattgcc gccgaccacg
    17581 gtgcagggcg gcacgtcgcg cgtcacgacc gaacccatcg ccaccaccgc cccctcgcca
    17641 atggtgaccc ctggacagat cagaacgttt gccccgatcc aggcatgcga ttccacccgg
    17701 accggcttca gatacgagac atggtcgtag ggcagcgcat cggcctgttc gtaccggtga
    17761 ttggacgtgt ggatctggac gtggggtgag aacgaaatgt tgtcgccgat ggtgatgccg
    17821 ccgatgccat ggaaggcagc gccgtgcccg atgtagacat agttcccgat ccggatccgt
    17881 tcaggattct caaatcgcga gcgcggcacg atttccacgt ggacgccgcg ggcggcgaac
    17941 gggatccgtt ccgtttcatg gcgcagatag gcccgccaga ggctccgcag gatgccggtg
    18001 atgggccgca cgatccagtt catggcgcgc cttcgctctt cgcgggctca gccggaacgc
    18061 ccgtccggct gaaccaggct tcggccgcgc gccggtattc gcgcagcgcc atgcggacgt
    18121 aggcctcggc gtgcgtcagg tcgctctccc ctcgccagtt gatctgcatc gcgcggtaac
    18181 ggttcagccc ggcgatcgct tcggcatagt tcatccggcg gtcctgcgag gcgtgcacgg
    18241 caatcgccgc gcgtttctga tcgatcacgg ccgagatatc caccgcgacg ttggccgggc
    18301 agtgcgacca gacttcatag ccccagaccg gcaacccttc gggcagcaca tcgtgcgcgg
    18361 cctccagaaa gatgcggttg gtcgcgatgt gatcgggatg cgcatccatg aagaagggca
    18421 gcaggacggc atcgggcagc aggccgtcga gcagctcgcg cagcggggcg gccagttcgg
    18481 gctgggacga caggcgctga tcctcgccgg gcagggaatg cacctgcgca acgcccagca
    18541 gccggcggca cgcctccatc tcctcccggc gcgtccgcgc cgtcgccgtt tcggtccagt
    18601 cggggcggcc ctgctccccg cccgtcatca ggacgatccg cacccgctcg gcgcggtccg
    18661 gcttgagcag gatcagtccg ccgcagccga tcgattcgtc atccgggtga ggcgcgagga
    18721 cgaggagcga gccctcgggc ggctcaaccc gcgcgggcga ttgcgccgca cgccagtcca
    18781 gcagcgcgcg cgcgcgcagc tcccgcccga acggtcggga gaggcccatg tagagccggt
    18841 cgaggagtcg aaacggcagt tccctcatgc atttggccct tgtgccgact cggccggcgg
    18901 cgcgttttcc ggaacccagg ccggttggcg gcccttcatc caggcatgct ccgcgtgcgc
    18961 gacggcccac cagtccacgc cccagtagcg cgtcccgcgc gtcaggtcga gcgcccgccc
    19021 ctccagcttg ccgtcacgcg cgaaggcggc gaacagatcg aaccagtgcg gggcggctcg
    19081 ttcacaacag cgccggaaca ggtcctcgat cagttcgccg cgaagcggcg ccacggcttc
    19141 ctcataatac acttctccgg catcgacggc ttcgaccgcc cggtggatcg tgaagccgat
    19201 ccgttcatga tcgccgcgcg ccagcgccca gtaaagcgtg tcggcgccgc gatagtcggg
    19261 tagcaggccg gtatggaagt tgagcgcgag cggcgcgacc gccagcgcct cgggccgcag
    19321 gatgcggcac cccatcatga cgaggatttc cggcgcggtc tcccgcaggc gccgcagtcc
    19381 ctcatcacgg ttgacattgg tcgtctggat cagcggcacg ccctgccgtt cagccaggat
    19441 atcgagccag ggcaaccgcg gattgtccgg ccgttcgaac cgccggcgca ggccgcgagc
    19501 cagctttctc gcggcgtaaa cccccatcct ggcccgcagc atgcggccgt atcgggccag
    19561 cagcgaaggc tccggcgcgc gccgccggcg gatgatcggg tcaacgacca ccgccgcggg
    19621 ccgaaacgac cgccgcccgc attcgcgcag caggagccgg gcgggcagat tatccagccc
    19681 ggaggtcagg atcacgacgc gcggacggga gctgacgggc tgcgcggggg cttcggccat
    19741 tgggttccgc tccagtcagg atggccatcc gttcgatccg ggacgggcgg caagcggcgc
    19801 gccgcggcgt cgtcccgcct cgacacacta ggttcatccg gccggttctg caaggcggcc
    19861 gtggcttccg ccgctgaccg gtcgtgaacg tgctgtggat tgtcgttcgt cggccgcgaa
    19921 tcggcccaaa agggttgcag cggcccggcg tttgccaaac aatgtcagat tcagcgcacg
    19981 gcgcggttcc tcccgaggca gccgcgttca gcgcgggggt tgcagcatgg cgcggaaacg
    20041 acccgaccct tcggaccaaa ccgccgactc ggttccggct tcgcgccccg caggtgagga
    20101 cgcggcggcg aagaaaaagc cccccgcgag agctgccaag agggaggctg ccgcgccgaa
    20161 atcagcgcgg aaggccgcga aaaaggactc gaagacgcct tccaccgaga cggcgaaggc
    20221 cccggcgacc aggcgatcca gggccgccaa ggcccaggcg gccgacaagc cggccaaaca
    20281 agccgccgcc aaggtatcca acacgaccag gcaagccgcg ccggctgctc tccccgcccc
    20341 accgctggcc agcccatcgt cggcgaaaaa gacccgggcg aagaaagaac ccaagacgaa
    20401 ggccgtcgcc ggcgcaaagc cgtcctcccc gcgccggccg acccgatcgg aatggccgga
    20461 ggccgacgaa gcggtgcgcg atgaccgctg gccggcgccc cgccgcgtcg tcctccccgc
    20521 cggcagcccc cgttcgttgg cggcgcatcg cgccgaacca ccggtcgcgc cgccgtcgcg
    20581 cgggcgggcg gcttcgaagc cggcagcggc tgaccttcct tccgacctgc cgcggacgac
    20641 ggccggcaag tcaacggaac ctccaccggg cgcgccgccg gcggcgacgc cccgggctcc
    20701 cctccccccg cccgccgccg tcccggccgg cgacacccgg agcgcgcccg cgcgcgccga
    20761 gcagatcacg acgatgccat ccgcctccgc cagtcagatc gtgctgatgg tgcgagaccc
    20821 ggagacgctc tacattcact ggaccctgga gaatgaagac ctgcagcggc acgggctgga
    20881 ccggcgggtc gggcgaccgc tgctgatgct gcgccttgcc gcggccgacg ggcccatcac
    20941 cggcgggttc gtgcaggaat gggtggttgg catcgaaacg cgggaccata cgctgcgcat
    21001 cccgcgtggc gaacgccact ggagcgtcga gctgggttat gtcgaccgtg acggccggtt
    21061 caggaccatt gcgcgctcca accgggtgat cagcccgcac ggccaaccgg ccgagctccc
    21121 ccacgaggcg cgggtgacgc gccgggccgt cccggattgg gttacgggtc agccgcggtc
    21181 gcccgcgccg gcgccgcgac acgccgatgt tcggctcgcc gccgaagatt cgcggcccac
    21241 agcccggcct ccggaaggat tgcccgagtg gctggccgaa atccccccgc cacaaccgac
    21301 cgggcctgac ccgtccacgc cgacgccggc ggccacgaca accgcggatg ataccgccca
    21361 tcggccggga gcgcatccgg ccagccaaag ggtccagggg gcgcaggcga aggacgtcgc
    21421 cccggcgccg gtggatgccc tcggcgctcc cctgccccag gtcgcagctt cccagccgag
    21481 gctgggtttt gcggccccgg ccggtccgtc cggagcgccg cgcgccgaag cccccgctct
    21541 cgatcacgtc gcgcaggctt cgggcgcggg attccacggc gccgggcaat ccgccgcgct
    21601 gccggttgat gctttccgct taagcgtcga tgccgatctg acggtttatg gggccaccga
    21661 gcccgatgcc cgactgaccg tgcagggccg gcccatcacg ttgcggccgg atgggacgtt
    21721 tacgctgcgc ttcagcctgc cggagggaga gcaggtgatc gatgtcgtcg cgaccagcac
    21781 cgacggccgc cacacgcgta ccgtgacgct gatcattcgc cgcgccaccc tttaagtgac
    21841 cgaccccgtt ccgtcctgat gttccccgcc gcccatgcga tgtgaccgga gagcgtcaaa
    21901 ccaaattgca aatagatctt attatcttat acttgcctca gcctcggtga acagggccgt
    21961 cggccttgct gtcttgacct gacttctctc caaaggagtt cgccatgaac tcacgcgagc
    22021 gtgttcttgc cgccatccgt cacaaggaac ccgacagggt tccggtcgat ttcggcgcca
    22081 tgcggtccac cggcatcacc gcgctggcgc acgttcggct caagcgccac ctgggcctgg
    22141 cggggccggt tcaggtctac gatctggtgc agcaactggc gcagcccgag gacgaaatcc
    22201 tggatcgctt cggcatcgac gcggtcgatc tggggcgtgc ctttctcact gatccggccg
    22261 aatggcgccc gtggacgctt cccgatgacg ccccctgcct cgtgccggcc tacttcaacc
    22321 ccgaacggca ggaaaacggc gactgggtcg cgcgcgatgc cgacggcgat atcgtcgcgc
    22381 ggatgtatca cggcgccttc tacttcaatc aggagatcca tccgcttgag gacgtgccgc
    22441 cggataaatg ggccgatagc ctgccggcgg cgatggccaa agtctcctgg gcggcgctcc
    22501 cctcgccccc gtatcacatc ccgctggacg acgcggggct ggcgcggatc gccgaaacgg
    22561 cgaaagcctt ccatggtcag accgaccggg cgatcatggt cgccttcggc ggcaacctgc
    22621 tggagtgggg ccagttcctg cgcggtttcg gcaacctgct gtttgacttg atggccgaac
    22681 cggcggccct ggagcgcctg ctggatcggc tggtggaact ccatctggag aacctgcgca
    22741 agatcctgga tgccttcggc gacgcggtcg atctgatcca gttcggcgac gacctgggga
    22801 ctggcgccgc gttgcagatg agtccggaga tgtatcggcg cttcttcaag cctcgccaga
    22861 agaagttgtt cggttacgtg cgcgaacgcg gcgggccgca tgtcttcctg catagctgcg
    22921 gttcgattcg ggaaatcatt ggggacctga ttgatgccgg cgtggaagtg ctcaatcccg
    22981 tgcagacttc ggcccacggc atggatccgg cggaactgaa gcgcgagttc ggccgcgacg
    23041 tgacgttctg gggcggcggc tgcgacactg cacacgtcct gccgcgcggc acggttcagg
    23101 atgtccgtcg ctgcgtgctg gagcggctgg agatcttcag caagggcggc ggctatgtct
    23161 tcaaccaagt gcacaacgtg acgcccgaag taccgccgga aaacgtggtg gcgatgttcg
    23221 aagccgtccg tgagttcaac ggcggctagc cgcgcaccac ggccccgacc gatcagtgcc
    23281 ggattgcctg aatcagttcc cccttgctca tgcggctgcg tccgcggatc ttgcggtcgc
    23341 gggcaatgtt atacagttca tccctggacc gctcatcgag cgaacggcgc ggattgccgg
    23401 ttccctgagt cgtcttgttc ggcgtgcggc cttcttcgcg acgctgcttg ttcacggtgc
    23461 gggcggcgat ctccctggcg cgcttcccct tgatgccccg ctcccgattg ctctccttga
    23521 tgtgttcata catccgttcg tccttgttgc tccacgatgg catggtcctg gcctccttgc
    23581 gaagatagga catggctgaa cacgaaccgt gccgggcaac gactgggagc gtggggacgt
    23641 ccccacatcg gcgggcggga aagcaccgaa attcgccgcg gaagaatgcc gcggatgacg
    23701 agatgctcgt gagccggagc ttcaaaacga ggaaagatag attggggcgg acctatgggg
    23761 aatcacgagg acgagggacg gattcttaag cgggagacga atcgccggaa tcttcgcttt
    23821 gggcgacgaa cggtttgaga cgctcgagcc atgtcccctc cagacgccgc gggcggccgt
    23881 cggctccgac cagaacatgg cgcgtccggc cgcggcaaag cgcgcgatcg tctccctcgc
    23941 ggcacagttc gtagcggaaa tcgaagcgca ccttgcccag gtgctccagc caggtgtgga
    24001 tcaataaaag gtcgtcgtat cgcgccggcg acaggtagtc ggcgctcaca tgcacgaccg
    24061 gcagatagta gccgtcgcgc tccagcaagc ggtattcgaa accggcgtcg cgcagcatct
    24121 ccgtccggcc gatctcaaac agctccagat agtgtccgta atacaccacc cccatctgat
    24181 cggtcatccg atacgtgacg cggtagcgac tgatatgagg cgccggccgg gaaccggcag
    24241 acgagtcagc ctcggacatg gagcgggctc cgagagggcg ggattgcgtc ggattgtggc
    24301 gagagacgga agcggcgggc ggccggcgac gttcaggaaa gggtgtcgac cagccgcccg
    24361 gcaagatgcc tgccgtgatg gatgctgcgc ggccgaacgg gcggcctgcc cgccgttcac
    24421 tcagcgctta tgccgcatcc tcttgcgcag cttgcggtac ttgtgtttgc gaatcttttt
    24481 gcggcgtttc ttgacaacgc tacccatgcg tgcctcgctg atctcaggat tgggcgggcg
    24541 gcccggctag gaggaccggc gatcccatct gcgccggctt catcggacag gcgcaaagtt
    24601 ggtatgctac ggttcgcgcc ggaaaagatc aagcggcaaa ccgcgtctgg cgtggccgat
    24661 gccgccggcg aaccgttctg aaaagcttcc cgtccttcgt ctggcgctcg ggggctccga
    24721 caatcaatcg ctgtccagcg ccagatcgac gaacctggtg aattccccgc gaaacagaag
    24781 ctgggtgtcg ccgatcgggc cgttgcgttg cttgccgatg cgcagcgtcg ccagttcggg
    24841 ctcgtgcgag ccgtcctcgt cggtcgccat gttgcgttcg cgatggataa acaggacgat
    24901 gtcggcatcc tgttcgatcg cgcccgattc gcgcaggtcg ctcagtcgcg gcagcttgtc
    24961 cttgacgccg cgcccctggc gctgctcgct ctggcggctg agctggctga gcgcgattac
    25021 cgggatgttc agttcccgcg cgatctgctt gagcgaccgc gaaatctcgc tgacctcctg
    25081 ctggcggttg tcgtagcgcg ccccgccgcc ggtcatcagt tgcaggtagt cgacgataat
    25141 cagcgccagc tccggacagc gcgacttgag ccggcgcgcc ttggcgcgca gctccatcgc
    25201 actcaggctc gaggcgtcgt cgatgtaaag cggcgccttt tcgatccgct cggccgcttc
    25261 gtggatcgcg tccagttcat cgcgccgcag gccgaagttc ccgcgcacgc gtcccatcga
    25321 aacgcgcgcc tcggtgcaca tcaggcgcgt gatcagctcg ctggccgaca tctccaggga
    25381 gaagatcccc accgtctggc cgcgccggaa ggccacatgc gccgcgatgt tcaacgctag
    25441 cgccgtcttg cccatcgacg gccgcgccgc caggatgatc aattgcgatg gccgcagccc
    25501 ggtcgtcatc cggtccagca cgcggaagcc ggtcggcagc cccggcagtt ccgccacgcc
    25561 tttcttgcgg ttttcaattt cgatcaactg ctcggccagc ggctcgccga tctggacgaa
    25621 gtcgcgctgc acccgatcca gtgcgatttc gaagatccgc ttctcggcct gctcgatcac
    25681 ctcgtcggcg cgggcgtcgg agctcgcggc gacttccgaa atcaattgcg tcgcccggat
    25741 cagcgagcgc agtttccact tctccatgat gatccgggca tactcgggaa cctgggtcag
    25801 cgagaagacg tcgtcctcca ggctggccag atacggcgcg ccgccgacca gttccaggtc
    25861 accctggcgg ttcaattcct cggccagggt cgtcagatcg atccccgtac cggcgtcata
    25921 gagccggcag attgatccga acacgcgctg atgcgcctgg ctgaaaaaga gcggctcgag
    25981 gtggttttgc ccgcggcggc gcgcggcgtt cccctggcgc gccatgctga ccgcctcggg
    26041 atggaagatc tccaggacca gatcgatctg gcgattgtcc aggatcagac tgcccagcag
    26101 ggcgcgctca acctgcaggt tatgcgggag cgagagcgtg ggagagtcct gcaggaggtc
    26161 gtccgacatg gcgacggggc cgacttgggt aaggggtagt tccgcctcga gggcgggagg
    26221 aggcccctaa tatcgcccag cgcccagccc cttgcaagga tttgtattaa cttgatttta
    26281 tttgacttag tcgcaaacaa aggcgcgggc gccccctccc gggaacgccc gctgcagatg
    26341 aaaatccagt cgattcagcc ctcgccgcgc agctggatca ggaatttccg tcctcggctt
    26401 cggccgcatt ttcctcggcc accgccggcg ccgcgacttc acccgattcc tgcgcttcct
    26461 gctgctcgcg cagcgcgcgc cggcggtccg acgcggcctg cgccgccgcc gccaggtcca
    26521 tatcggtctg ctcgctttcg ctcaccgtcg tctcggcgtc gatctgcacc ttgatatggc
    26581 cctcgatgtg acccacgagc ttgatccgca cgtcatgctc gccggtcgtc ttcagcggtt
    26641 cgtgcagcgt gatctggcgc cgctcgaagg catagccctg ctcgttgagc tgctccagga
    26701 tgttctgggc cgtgaccgag ccgaacagct tcttgccgtc gatcgtcttc atcgtgaagt
    26761 gcaggcgcac gccggcgagc cgttcggcga ccgattcggc gtcggtccga tccttctccg
    26821 cctcgagctg caacttcttg cgcttggcct caagccggcg cagcgtcgcc tcgctggctt
    26881 cgacggccag cccccgcgga ccaaggtaat tgcggaagta gccgttggcg acattcacca
    26941 cgtcgccctg catccccagc ttgtcaatgc tccgaaaaag aattacctgc atctgcctca
    27001 ctcgctgttg acatggccgc gcgcaaaccg cgggcctgat tgacggctcc tggcgcccgg
    27061 ggacgctcag gaggccccgc gccgttccag ccggcgaaaa tccaaccaga catccatcag
    27121 ccccagcagg gcgccgatcg gcacccggat aaagccgatg aacaggatca gtccgcccca
    27181 cgtcatggcg gcggccagac ccggctgccc catcttgcgg cgcagcgcgg cggtaaacaa
    27241 caacaccgca atcccctgca ccagcgccgc gacatacatg accgcgagca ggggcagagc
    27301 aatccgccac gctgtcggcc atgcgtacaa gctacccaag atcgcgaaaa acagcccggc
    27361 gatcaagaca aaaatatagc gctggctcaa ccggaacagc accagcagcg agcgcgacgg
    27421 ctggaaccgc ccgatggccc gccgcacaac ccggcccgtc aaccagagcg ccagaaccca
    27481 cagcgccacg agcgtcggca cccaggcccg cggcagatat tcgcgcaact gctccagctg
    27541 ggttgggttg aacagcggcg cctggcgctg ctcgatcagc cgcgcgttct cctgctcgat
    27601 caggttcaac agctgtgtcg ggctctcgcg caacgcctcc agcggcccat ccagccggcc
    27661 gccctggccg agccagacca ggatggcgaa cgccgccagc gttgcggccg ccggcaggaa
    27721 aaagtagtcg tcttcgcgcc caccggcccg gttcagccag acgcccgtcg cccccgccag
    27781 ggccgaggcc atcacccaaa gaacggccag ctccggcaga acataatagg tagcgaggac
    27841 gacgccgatc agcaccaggc ccaccagcgg cggactggcg cggtcgaacg cggcccaata
    27901 cgccgccgcc gccagacaac ccccgacgat cccccccgcc aaacctacgg cgctgatcac
    27961 gatggccagc accgtaacgg ccgccagggc ggcgcccagc agcaagccga tccaccatgg
    28021 cggggagcac ccggcgccgc gcgcccgcgc gcgataccgc gcgggaagtc ggtgcttctg
    28081 cggaccggcc gccggcgccg ggccggccgg atcgggtggc gaatttgcgt gggacgggtc
    28141 agccattgat caacgtgacg cgtggagtcc cctcatgcgg gtattcgatg acgctgaccg
    28201 aacaatgatt caacgagaag cggaaacagt tgtcgagcgg catgccgagt gcgtcggcca
    28261 gcgcgacgcg gatcggcccc gcgtggcaga ccagcgcgat gcgcatcccc ttgaacgact
    28321 gggtcagttc ctcgatcgcc ggcagcacgc ggtcgcgcag atcgacaaaa ttttctgctt
    28381 cgggcacccg gaaattgacc cgatccaacc gccattgccg gtaaatctcg gtgtgttcgt
    28441 gcgccgtctc gaggagcttc acatccagcc cctgcaggat gcccatatgc cgctcgcgaa
    28501 agaccgccag ccggcggacc ggcaggccgc gcccctccgc gatcagctcg gccagatagc
    28561 ccgcccggcg cagatcgctg gaataaaccg cgtcgaaggg caccgccgcg agccgctcgg
    28621 ccagccggcg cgactggtcg cgtccgcgct cgctcagcgg cacgtcatcc tgaccgtaat
    28681 agcaattcag gtagcaggct tcgggctcgc cgtggcggat caggtaaagc tgcgtcgggt
    28741 tggtccgcgc cagggctccc ggcgccagct cctgaccgct atcggtggta aacgacatgc
    28801 cgcccctctc gcgcatcgat cggtcgcgct atcgcgcacg cgaatccgat cataccagac
    28861 cgcgcgggtg gaagcgaagc ctccgaatcg gtggcatcgc ggaggccgcg agccgttgcc
    28921 ggcgcgcggc cggaccgccg ccgcagctat tcaccgtcgc ggccggcgcg caactgcgcg
    28981 cgatgcgacc gcagtaactt gagcgtctcg cggatcgtca ggcccaggtt catcttgctg
    29041 gcaccctgct tcaggtcgta gcgcaggatg aacggcacct cctcgatccg ggcgcccacc
    29101 atcgccagat gcacgagcag ttcatcggtg caggcaaacc cggtgcgcgt gatcaggccc
    29161 ccgggaccga accgcgccag cgcgcgccgg agcaggctgg cgcgcatcgc ccggaagccg
    29221 caggtgtagt cgcgcacgcc cggcagcgcc agatagtgcc ggaacaggag ccgcgcccca
    29281 aagctgagcg tgcggcggaa gagcggcacg ccgtgctgcc ggctgccggg ctggtagcgc
    29341 gacgcgatca cgacgtcggc ccccgtcgcg tcggcgcggg cccgcatcag ggggatcgtc
    29401 tcgggcgggt gcgtcatgtc ggcgtccatg cagacaatca gccggtccgc cgcccacggg
    29461 ccctccagcg cggattggat gcccgtcagg atcgccggcc ccaggccgcg attccgatcg
    29521 tggcgcacca gccgcagcca ggggcgcgcg gccgccagcc gttcgacaat cgccgccgtg
    29581 gcgtcgcgcg agccgtcatc gacgacgatc accgaccatt ccagcggctc gggccgcagg
    29641 gcctgctcgg cgcgaatcag caacggttcg atcgccgctt cctcattgta ggccggcaag
    29701 gtcaggcaga tgctgcggat cggctccggc acgtcgggtc tccacgcaat gtgtcggctg
    29761 cgttcctggg cgcccggcgt cgggcaaggt cacaaaaagg ccggcgtcgc gcgacgccgg
    29821 cctgtatccc gaatgatctt ccaacgtcgt tactggaaca agtgccagtt gcccacggcg
    29881 ttctgttcga tgatcacaag cggcttgtcc gcggtaaaga tgttgttctc gttggattca
    29941 tccttctcgt gatcgtagaa gtccacgagc acggccagcg tgtagctgcc caggtccaat
    30001 ccgctggaat cggcctggat ctgctgcggc accgaggtgc ccgggccgat cggattggtc
    30061 caagcttcgc ccaggacgat gtcgttgccc gaaccggtga tctcggcgtc ggggctcgcc
    30121 acaacccgcg accagtgccc gtcgccggtg ctgctgccgg ggttcgtttc ggtccagcgc
    30181 accagaaacg gatcgccctg ttcgacgaac gccggttcgg cgaccagact gcccgcctcg
    30241 atggccaggt cgggaacgcc cgtgctgaag acccagatcg gcccggaagt cgtgatcacc
    30301 gtctcggtcg ttagccgatt cttcgccgtg atccgc
//
LOCUS       DAOPCO010000018        29620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1717_length_29620_cov_5.722205, whole genome shotgun sequence.
ACCESSION   DAOPCO010000018 DAOPCO010000000
VERSION     DAOPCO010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29620
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1717_length_29620_cov_5.722205"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PLS90_02705"
     CDS             <1..689
                     /locus_tag="PLS90_02705"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPK01345.1"
                     /translation="PGMSRSDLLGMNARIVGEVSTHIKNHAPDAIVVVVSNPLDIMCS
                     VALEMTGFPKERVIGMAGVLDTTRMRCFIADELDVHAADVVALVLGGHGDSMVPVIST
                     ATVSGIPLTQLLPRERIDAIVERTRKGGGEIVALLKTGSAYYAPAASAVEMVESILLD
                     SRRVLPCAALLDGQYGISGCFVGVPVKLGARGIEQIYEVKLADDELKALQASAAAVKE
                     DLDALKGMEY"
     gene            724..846
                     /locus_tag="PLS90_02710"
     CDS             724..846
                     /locus_tag="PLS90_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01346.1"
                     /translation="MGFHSQPYRLVDGYYQPVIEVELLGPAGGTMQLVLVDSPQ"
     gene            1000..1569
                     /locus_tag="PLS90_02715"
     CDS             1000..1569
                     /locus_tag="PLS90_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPK01347.1"
                     /translation="MIQNPANLVLPTSLFGGGLVLPYLDFQPVLGENVFIAPNASVIG
                     RTTLGDDVSIWFGAVLRGDIAEVRVGRGSNIQDNSVLHVGDDDPCIVGEEVVVGHKVM
                     LHGCTVENGCTIGMGAIILNKAVIGERSVVGAGALVTQETIVPPRSLVLGSPARVKRE
                     LTDAELRKIHANFAAKYVKVAANYRRVLG"
     gene            complement(1627..2133)
                     /locus_tag="PLS90_02720"
     CDS             complement(1627..2133)
                     /locus_tag="PLS90_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01348.1"
                     /translation="MGVLELIQQKAFLGKEFLTWLWFKSETGGGVNLKGGAIQAEILG
                     PIVLDAQYGDARATGLKGESPATSPEARTALVEGKKLKKAKIRLKREDVEWVATLDGE
                     NFNLAGLAIPNPGRMPFEDMMALRTEMMLEFEHIIEGLMQAFLELRLDAKEWAKELAR
                     IQTWVREK"
     gene            complement(2139..2753)
                     /gene="grpE"
                     /locus_tag="PLS90_02725"
     CDS             complement(2139..2753)
                     /gene="grpE"
                     /locus_tag="PLS90_02725"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPK01349.1"
                     /translation="MGERIYGVDHRLARRLRRRVYRATEAETAAAAHLCDEHGHSHHH
                     GHGDHGGHRHHGPGAVIEENEQLERLKDQLLRAQAEFENYRKRVRRDQEQQIAQAQMK
                     LIEQLLPVLDNFERALANPGESVEALLQGLQMVQKQMSQVLEQNGLEKVPAMGQPFDP
                     NLHEAVATDPADAEHPENTVTEVFQDGYRMGNRLLRPAMVKVAR"
     gene            3098..5596
                     /locus_tag="PLS90_02730"
     CDS             3098..5596
                     /locus_tag="PLS90_02730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK01350.1"
                     /translation="MSAATPPPPPPGRRVPASATPRAKPPAARSGRPRARLNRRSACW
                     TAHLAAGLGCGLALLFTAWFARTNPALIRLFQYLTEEWHFGSGFEWRMLGWTAAALFL
                     FVVLGRFLLECFELYLPRSATLALSFLFGMGLAGFVLELLAIPHWLYRAPMAAALGGL
                     LGLLWLGAIWCGRRRPASGLGGEPGYNETLLRRALARAAWRAAVTRPRGAAWLLAVPL
                     VALIVAIQFFIFWHAVLYPEVYWDSLILYLGYARMTFLEHGFHTKVTGQVGFGLGANY
                     PHLFPVLGAGIATAAGQWSELPQRIIAPLCGTATTLLVYHTGLRLTRHVIFSLILTLL
                     YRAVPLGIIYDQYASDYALALLFAAAFLYLCLLYLQTALPGYLAAAAILTALAMHLNY
                     LMGILWLPWVITLWGAHAGAPRPDPRRNPELEGAPWTALPARPPLARALRRGWLWGIL
                     LLSCVIGSTWYIRNWIVTGNPVYAFFHEWLGGKHINPEVMEAAAHEWRANGAGIGRLG
                     DSPMERLRAAFTFFAGPGPGFFWHQYYRTEPLLLGFTLFGVLAWLGRWISGCLSGRSR
                     RRRRAHRVLALRFGWVVASLLFGLLAFHFVLAPFYLYQIIMILPCAALMVAFAWPWWR
                     LPLWRTVLLLLALWVGVIPGLAMAMMGFKIVGMFQLSADRTESPLTLYPLRHPLPRPE
                     VFYRWRFGEDARMWDYINRNLRGERLLTHDNRHLVYDPSITLVHLDDWTDEWNIQALW
                     GLPNPADRVRQLIQLHNIRYYLFIPNELAAATNRHMQTEQWVELHLAELIFQAGENRL
                     YRLRLPPEDQPLAAPTTPFAAGAPAADQPTTATP"
     gene            5740..7422
                     /locus_tag="PLS90_02735"
     CDS             5740..7422
                     /locus_tag="PLS90_02735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK01351.1"
                     /translation="MPTDADPDPAPSTSRSPEGTEQPDLPLDDSPLDIQHLVQPAEAL
                     PPLQLADLPDPLRQAALHAGWKELTPVQQYAIPYMLAGRDLMVQSRTGSGKTGAFLLP
                     MLDRIDFTRPKCQALVLVPTRELAQQVTREAQLLSQSAGLRTIAVYGGVGYGAQLDAF
                     RDGAHLVVGTPGRVLDHLLRGSLKLDQLRFLVFDEADRMLGMGFYPDMKEVERYLPAR
                     REGYMFSATFPLSVQRLAHQFLNQPGFLSLSRDNVHVTDMDHIYYVVPPMEKDRCLVR
                     LLEIENPGAAMIFCNTRTRVNYVTAVLRRFGYDVDQITSELTQVARENVLARIKRRQL
                     RFLVATDVAARGIDIPHLTHVFLYEVPEETELYIHRAGRTARAGASGVAISLVSSLEE
                     LDLRRIATRFKIELEKRPMPTEEQYNEIVAQRLVALLESRLRHRDRLQVERMRRMLPL
                     ARALSETDDELALLAMLLDDAYQDLLHAPPELPPPEKDADEPKAGKRRRSRSRGKGKA
                     PAGPGEAAAAEPQAAPSPPPERRQPDAPPTDAPAERKPGARRRVVRRREQSD"
     gene            complement(7468..8256)
                     /gene="recO"
                     /locus_tag="PLS90_02740"
     CDS             complement(7468..8256)
                     /gene="recO"
                     /locus_tag="PLS90_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPK01352.1"
                     /translation="MPALDAIVLATSPYRETTLIVSLLTRSEGVIRVLARGARRKGKV
                     QAAFEAYAWVRGEFRLPAASGKLGTAGGVGLVKSWDYLRRDLARLAYAGVGLETLGRL
                     AEEAPPEPYYFEEACRFLTVLEDAAGPGSVLVALLLRLLHHAGFPPHPGEGLDPAAPP
                     ATCFHHFATHRLEIARPEPDRHDGDAHRYHVMPLSGEMVRAIGEGLRRPPPLDGSWQL
                     PARAGREALTWLIRVWEDHLNHVLKSAEFLERMVLGSRARRGPR"
     gene            complement(8385..10349)
                     /locus_tag="PLS90_02745"
     CDS             complement(8385..10349)
                     /locus_tag="PLS90_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPK01353.1"
                     /translation="MDRARTRSWPALLVQVVLLLTVACDAGGRAERRGGGDAAAGGES
                     GPATVRISGVVQLEGRDDSSGVQVFIPGSSLVAMTDAAGRFELSPVGEGLYELQARAA
                     GFHGAKLGPVQVTPADAGRTIPLDPLVLQPETAAAEPAGAALGAIAGDVVAGDEMAGE
                     TINWAACTISLENTPYRTTCDSDGSFLLWNLPPDRYQLRATMPGFQTWTQSVRVLPGE
                     PDERLVIELTPAGNYGGGGPGHDRRIRGVVTLTGPDGEPSNAFDQVLIEIANHPELKA
                     ILENDGTFEFPSLAPGRYVVMASGIGFEDARPVEVDLTNAPAVDVHLELRAEPGIEPA
                     GAVIRGIARKNDNEIRDMSGIQVALIGTSHAATTNAQGEYVIDEIEPGIYDVIAQADG
                     YEPARLGPVELAAGEEVNLEEMILEPIRDYPEVLSVSPADGTRGVLVREVVPVRVRFS
                     KKMDLPSLRRAILFDPPVSFEVFAGGGKAGPDTDFVQIELAGSEGDPVALLNQRYRMT
                     IGVTAKDVEGLTLQEPFEAEFSTAGPAVIRTVPADEGLKRSLSPSEPVSVFFNTRMDP
                     ASLGSDTLRFRPRLRMAPVLSVQEDRATGWSILNIQAQWEEDTEYSITIGRRAETFDR
                     DKLDNAPYTFRFTTPELREFEPPRAPQGVR"
     gene            complement(10380..12389)
                     /locus_tag="PLS90_02750"
     CDS             complement(10380..12389)
                     /locus_tag="PLS90_02750"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK01354.1"
                     /translation="MKRFALLLVMLLVAIPAGYAQPMGPMGEATTDTLNTESIQFLEE
                     PRPDEAVAARNAWGELTIRNLMDYGSANPVPWLRGNLSLNQIAALPGDLIMRLVDHIS
                     ARQAAMHDLRNHPELVYYNPMVEQWERTRDQLIVNRFVETELEAKLVEPPEAVVTAYY
                     EEHKPEFKQNFGFKMRHLILLTYEPYIVAEGDTLESIAEKISGDAANADNIRADIAGK
                     PARREEGEVFKPLVPGEQLLVPMDEADAQQVRQRLEEILKELDEGKTFEELAKQYSET
                     EPEARGQVIGWLPAGTRDMLPDLLEMGRQTPVGEISPIFRTKHGWQVIEVVDKREEGF
                     RPLEDVRSTIEMRLKQEKRDELIQELLDRLMLNPALEINYDALTTDADATTDTMTVVR
                     RTEPSTDTVVASVGDRKIYYGEIAQGWSQVSPPIDREKVRGFLREMGKLQINLIVEEM
                     RPFYDDPASTLARQLEWMEVGFLGSNKINHVARLRAMDAITDEMKRNYFAEHREEFAT
                     PEMVAYSTMERRLSPEQQALEGEARQAAVDQLKEMMTADLADVKSAEDFINLAGQVNE
                     TLLQQGVRIPQAADPVPVDRIEEPIAAQIAELQEGEFSQPFVVGDQRVLSVLVEKRQP
                     AGIPEYEAVASQVENRLLPQLFREYGEAVEREYLEQAQVEHLITE"
     gene            12625..13533
                     /locus_tag="PLS90_02755"
     CDS             12625..13533
                     /locus_tag="PLS90_02755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01355.1"
                     /translation="MPPTPSRSFHVLVVLYNSAQWIDGCLRSALAQTLPPARITVVDN
                     GSSDGGPELAERIGDPRIHVIRAGKNLGFAGGNNRAAAAARDMDFLLLLNPDAVMAAD
                     TLQRLADAFQTDPAIGVLGCKVLEPDVATIQHVGIHLRGNGLPSNLGQGEPDNGQYSG
                     IHDVASVLGAAMAVRADTWCELGGLDEAFWPGYYEETDFCFRARRAGWRVAVARDAVV
                     THFDASADRRRDPEFLRLFFRNRARFLLKHYRARDWLFRYLPDETRWLTYWGSRQMRR
                     IALRSLWQVRAEMRRERKLAARRWSP"
     gene            13533..14006
                     /locus_tag="PLS90_02760"
     CDS             13533..14006
                     /locus_tag="PLS90_02760"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HPK01356.1"
                     /translation="MLHVVLYAPRIPQNTGQIARACFAMGCRLHLIRPLGFRLDEASL
                     KRAAVGHLKATDLRLHRDGDAFWSSPDLDPDRCWLVTKHGSVDYAQVQYRQGDWLIFG
                     NETEGLPPDWLERRRDRTVVIPMHEPRVRCLNLATAAAAVLFEALRQLRLTQEHG"
     gene            13999..14904
                     /locus_tag="PLS90_02765"
     CDS             13999..14904
                     /locus_tag="PLS90_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01357.1"
                     /translation="MADPAATRPRVDAIIVNYHQPGLTQAAIRSFLDSDGIDPRVILI
                     ENEGDGAWAGDLLAREPRIELIANRANVGFGAACNQGFAAVLRGDAQFVLLLNNDAAA
                     ATADAVLRLIDPAARAGLAAPRILLPDGRIYAAGGRVEPWRARCRNRGFHEPDAGQYD
                     RPNETPFASACALLIHRRVLEAGLRFHAPYFLYYEDADFCLAARRAGFRIRYVPESRF
                     IHHESASTATPLRPRLLFYTTRNRLLFLRRNYGWPIRLVGGVYLAGVVAVRQIVGPLR
                     GRGDAARATARGYLAGLGLLSGADK"
     gene            complement(14937..16421)
                     /locus_tag="PLS90_02770"
     CDS             complement(14937..16421)
                     /locus_tag="PLS90_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HPK01358.1"
                     /translation="MSCAELPRLTADEAVTHIQHGDTVVTSGFTAAGTIKACGRALAN
                     RARAEHEAGRPFKIRLLTGASTGPSVDKALAEADAIAFRAPYQSDRTLRQRINKDQVP
                     FFDIHLSHVPQYVEYGFFGEIDVAIIEATSISREGHIYLTTAGGASPTFLKRARRVII
                     ELNRFHLPRLREMHDIFVPALPPRRREIPIFHPLDKIGTPYAMVDPKKVIGIVETDEP
                     DESGSLDPYTEIHEQIGRNVVEFLAREMKAGRIPPEFLPIQSGVGNVANAVLANIGLD
                     SRIPPFYMYTEVFQDACLQLMDADRLLGASTCSLTLSPDALHRLYGEFDRYADRLVIR
                     PQSMSNNPEIIRRLGVISMNTALEVDLYGNVNSTHVGGTQMMNGIGGSGDFTRNAYIS
                     MFTCPSVAKDGAISAVVPMVSHSDHNEHSVQVLVTEQGFADLRGVAPADRPRIIIDNC
                     AHPDYKPLLHDYINRAAKGHIAHDLRHAFDFHIRFAETGSMMPK"
     gene            complement(16739..19084)
                     /gene="pnp"
                     /locus_tag="PLS90_02775"
     CDS             complement(16739..19084)
                     /gene="pnp"
                     /locus_tag="PLS90_02775"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010035466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPK01359.1"
                     /translation="MSMERISVPFGAEPLILEVGRMAKQADAAVIAQVGGTVMLVAVT
                     VSPEPLEGRDFFPLMVDYREKFYAGGRIPGGFFKRESRPGDLETLRARITDRAIRPLF
                     PENLRNEVMVYITILSSDNIHPAEIAALNATSLALRLSSVPFPTATAAVRVGRVDGEF
                     VLNPTFEQQKVSDIDLIVAGTRKAINMVEAGAEFVSEEDMIAALRFAHEAITEIIEDI
                     DEFAAQHGKPKFEIKVEPLPAEVAAHVRELCVERFDKLVHGRLSKEAFSEGEHTIIAE
                     TAAAIAVEHPELVGQVKDYATELLSQAVRKLIIEEGQRVDGRARDEIRPVTCDAPILP
                     AVHGSALFTRGQTQALGVVTLGTVGDAQMIDTMLGTYDKYFMLHYNFPPFSVGEAKPM
                     RAPGRREIGHGALAERALQPVIPKTEDFPYTIRLVSEILESNGSSSMASVCVGSLALM
                     DAGVPLKQPVAGIAMGLVANEEGKMAVLTDIQGVEDHLGDMDFKVAGTRDGITALQMD
                     IKIEGVTFEIMEQALEQAKAARLKILDIMTELLPEPRAELAKAAPRVSSIQIPVDKIG
                     MVIGPGGKTIRGLEERTGAKIDIQDDGKIFVSSPNGQSMEAARQLIQEMTAEVTVGQV
                     YEGKVVRVTDFGAFVELLPGRDGMVHISELEIGRVDRVEDVCQVGDSLKVKVVNIDPA
                     GKVRLSRKAILMEEQGLEYTPDDGGRRGGGGGGGRGGERGGGGGRGGDRGRGGERGGR
                     GDRERGPRGGGERGGRERRESSAEPKEYGFRERPRQKHEDD"
     gene            complement(19411..19680)
                     /gene="rpsO"
                     /locus_tag="PLS90_02780"
     CDS             complement(19411..19680)
                     /gene="rpsO"
                     /locus_tag="PLS90_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPK01360.1"
                     /translation="MSITLERKQQLIEQYKTHDNDTGSPEVQVAILTERINNLTEHFK
                     AHGKDHHSRRGLLRLVSKRKALLTYLADNQIERYRELIKRLGLRR"
     gene            20177..20572
                     /locus_tag="PLS90_02785"
     CDS             20177..20572
                     /locus_tag="PLS90_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK01361.1"
                     /translation="MHLTLDHHSGVPISQQITGQIKYLVVSGGLVPGERIPSVRALAT
                     RLRLNPTTVARVYRQLEAEGVIFTHPGRGAFIAGRPPGLTQAEQHRRLEGDIRRLLVE
                     AGRLGVSFADLLRWIEEEIGQIEQLRDAR"
     gene            20697..21008
                     /locus_tag="PLS90_02790"
     CDS             20697..21008
                     /locus_tag="PLS90_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK01362.1"
                     /translation="MNVIEAYGLTRYYRTQRGVRDLDLAVPEGCVLGLLGENGCGKTT
                     TIKLAVGALSQWLLKDRSNSRRAIQEIGVPRKNQDRHGPMSRGVSASKSCWQPKDFDI
                     N"
     gene            21195..22397
                     /locus_tag="PLS90_02795"
     CDS             21195..22397
                     /locus_tag="PLS90_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01363.1"
                     /translation="MIFESVLEAEKTMVAMPARAIRTFVWDFADEGVHEVLGRLADSG
                     VNGVHLALACHGGRYYCPHNPKRALVHAPDGALYFQPLLSCYDEIKPRVHPEFGSGAF
                     VARLIESMREYDMIATAWVTLFNNQSLSLAFPECTMVNALGDRLEGTLCPAHPAVRGY
                     AQALVEDLAHRVGVDMIELQDFAFPSHDQFIGARWVDVPVGQGLGYLLSLCFCEHCRR
                     RAEELNLEVDDLAQRVERMLHRALAGDLSERRLNDEIADPYNSLARYAEARAGTITTL
                     VDELNDATDGSHAGLQLVLAEEPDDAWRWGIELHTLRQRRLRATLQPGRGPAPRPTFV
                     SRYAEVLQLGRDLAVDIPLGVPHTDDYSPAGAIQAALESGIDHFVFSHYGLVPLDMLD
                     WIDALARR"
     gene            22397..23137
                     /gene="ispD"
                     /locus_tag="PLS90_02800"
     CDS             22397..23137
                     /gene="ispD"
                     /locus_tag="PLS90_02800"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPK01364.1"
                     /translation="MDPDDFIPPPPRVAVVIVAAGRGERLGELTPKQFLPLAGRPVFS
                     YSLRFFGGLPFVVEIALVHPPAGLPEAARAHVEGPAVPLRLVAGGARRQDSVAAGLRA
                     LRAPFDVALVHDAARPFPDRRATRLLAQRAMETGGGLVALPCPDTVKRAGNDGCVDAT
                     LDRRTIWLAQTPQAIRADYLGSALALMDSAEDWTDEAAVLERLGLRVALVAGSSANFK
                     ITTREDWLRAEALADREALARPGLNQPK"
     gene            complement(23291..24133)
                     /gene="pyrF"
                     /locus_tag="PLS90_02805"
     CDS             complement(23291..24133)
                     /gene="pyrF"
                     /locus_tag="PLS90_02805"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPK01365.1"
                     /translation="MELRTFFERLAARCDQTGASLCIGLDPDPTRLPRHLGSGPEAIY
                     RFCVEIIEATADHAAAFKPNLAFFEAIGVEGWHVLDQVLRAVPQDVPVILDAKRGDIG
                     STARMYARSLFDRLRAGAVTVNPYMGYDSVEPFLEFERNGTYVLCLTSNQGAFDFQLA
                     DDLYLRVARRVREWNTRGNCGMVVGATKPQFLTSIRAIAPEIPLLIPGLGAQGGDLEA
                     TLAAAPDAPPHHLLFNVSRSIIYAADDASFGRQARVAARYYLDRIQRARRKVLGLGTE
                     GAEE"
     gene            complement(24311..24913)
                     /gene="yihA"
                     /locus_tag="PLS90_02810"
     CDS             complement(24311..24913)
                     /gene="yihA"
                     /locus_tag="PLS90_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPK01366.1"
                     /translation="MKAEFVKTITDPSQAPPPRPAVVFAGRSNVGKSSLINKLTNRKG
                     LARTSSTPGRTREIQYFGIDDRYYLVDLPGYGYAKVSKTERARWGPMMERFLRKAEGI
                     RLVVLILDVRRDPSPEDLQMIQWLEHGERPYLFAVTKCDKVKRGERSRRLHALQRALG
                     LADGSALVPVSAATGEGIGDLWRVIESVLSPAPPEPKPPA"
     gene            25079..26293
                     /locus_tag="PLS90_02815"
     CDS             25079..26293
                     /locus_tag="PLS90_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HPK01367.1"
                     /translation="MSSAQRPILVQFGAGNIGRSLVGQLFSASGYEVVFIDAVPAIIE
                     ALNRERRYKVVTKGKTLPGQSPETLVEHVRGILATETEAVAEAVARADVIGTAVGPAV
                     LPRIMPAIAAGLRRRERPISIILCENLRGAAGIARAHLAAALPADFDLAGRVGLVETS
                     IGKMVPIMPTEARRRDPLEVWAEAYNTIIADREGFIGEPPAVAGLVCKRNFAPWVDRK
                     LFIHNLGHAAAAYHGFLRGHTYVWECARDAEVRRLVQGAMRESAEALVRAHPSEFTRD
                     DQAAHIEDLLERFGNEALGDTVFRVGRDLPRKLAPDDRCIGALRLCQREGVEPAAIVG
                     VIAAALHFKARDEHGELFPADAAFHARLASAGPETVLREVAGLVAAADARIVREILDQ
                     SRRMASAGNESA"
     gene            26428..27939
                     /locus_tag="PLS90_02820"
     CDS             26428..27939
                     /locus_tag="PLS90_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPK01368.1"
                     /translation="MHKPDNPPSDASPAAEKRPPTLMRHYVRYLNANILTIVAGFISY
                     PVMVRLLPTEEYGLFGYFQGFILVWIAVLKLGMQHSIRRLYPSHCLGRSIAEQKTFYT
                     TLVLAPAALSLVLFALTLAALHAVSLFRPIADLRYLEIILVISQIGVIQSLIDNVIRA
                     REESRLAAWLIIVGRYLELVFVVLVIVFLLRSAMGVYWARLAAVGLIFFYVVGWARRN
                     CHFDPRSFSGDLFRQSLRFGLPLVASEISFILLAFADHVMLRYYHGNTAVGVYSIGYG
                     LAITVGVVLRFPLEPAFQPVANRLYETKGIQPVLGLQRRVLHLLLYAVCAITAGLLAV
                     GRDFFLLISGAEKASSAPIFVWIAVNYTLFTVLIVWTHGLTLLKRTNRILLTVGGAAL
                     LNVALNIWLIPRYVEMGAVYSTLIAYGVMGLAQVALCPRDYLPRLLIGDWLRPLALAG
                     LMLAVAWGTDLFGLTASIPRLAAMAGVFVIVFVIPAWFIDREMREYLREKLGI"
     gene            28099..28554
                     /gene="queF"
                     /locus_tag="PLS90_02825"
     CDS             28099..28554
                     /gene="queF"
                     /locus_tag="PLS90_02825"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013615018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPK01369.1"
                     /translation="MARGTTSAKPEQPDKGAQGFKALDRKLEFQGLEAIDTAALEAFD
                     YQYPGKPITITTTTEEFTSVCPFSGLPDFGRITIAYVPDRRCVELRSLKYYLMSYRNV
                     GIFYEHLANRIRDDLMALLAPRSLRVECRMTIRGGLQTTIIAEHEQAAR"
     gene            28551..29138
                     /gene="nadD"
                     /locus_tag="PLS90_02830"
     CDS             28551..29138
                     /gene="nadD"
                     /locus_tag="PLS90_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPK01370.1"
                     /translation="MSGAGQTIGLFGGSFNPPHVAHVLVVGWALAGGAVDEVWVIPTG
                     GHPFGKPLAPFEDRLELCRRAFACFGPKVRVLDLERGPDAQYSIDTLRRLQARHPDLR
                     WRWIMGSDQLADAARWKNFDEIMRLAPPLVVPRAGHAVPGASGDGAPPRFALPDLSST
                     FLRERLAAGAFAELAGLIPGPVLDEIDKRGLYRPV"
     gene            29246..>29620
                     /locus_tag="PLS90_02835"
     CDS             29246..>29620
                     /locus_tag="PLS90_02835"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPK01371.1"
                     /translation="MRITMCKAKLHRATVTEAQLHYEGSITIDEDLLEASGILEYEKV
                     QVVNINSGARFETYTYAGPRGSGMVCLNGAAARLVQVGDRIIVIAYCELEPDEAREHK
                     PTLVLLEDDNSIKEVVRNGRHPV"
BASE COUNT         4473 a        10450 c         9473 g         5224 t
ORIGIN      
        1 agccgggcat gagccgcagc gacctgctgg ggatgaacgc caggatcgtc ggcgaggtca
       61 gcacgcacat caagaaccat gcgcccgatg cgatcgtcgt cgtcgtttcc aatccgctcg
      121 acatcatgtg ctcggtggcg ctggaaatga ccggcttccc gaaggagcgc gtcatcggca
      181 tggccggcgt gctcgacacc acgcgcatgc gctgtttcat cgccgatgag ctcgatgtgc
      241 acgcggccga cgtcgtggcg ctcgtgctcg gcggtcatgg cgattccatg gtgccggtca
      301 tcagcacggc gaccgtctcc ggcatcccgc tcacgcagct cctcccccgg gaacggatcg
      361 atgcgatcgt cgagcggacg cgcaaggggg gcggcgagat cgtcgccctg ctcaagaccg
      421 gctcggcgta ctatgctccg gcggcctcgg ccgtggaaat ggtcgaatcg atcctgctcg
      481 actcgcggcg cgtgctcccc tgcgcggcgc tgctcgacgg ccagtacggc atcagcggct
      541 gcttcgtcgg cgtcccggtc aagctcggcg cccgcggcat cgaacagatc tacgaggtca
      601 aactcgccga cgacgaactg aaggcgctgc aagcctccgc cgccgccgtc aaggaagacc
      661 tcgacgcgct caaggggatg gaatactgag tcggcgcggg cggctgcgtg gtgaatcccc
      721 agcatgggtt ttcatagcca gccctatcgc cttgtcgatg gctattacca accggtcatc
      781 gaggttgaat tgctcgggcc ggctggcggg accatgcagc ttgtgctggt ggattcccct
      841 caatgacccc gttctggaca aggagacccg ggatgaccgg atgcgcgaca tcgacagcct
      901 gagatgacct ggccgcggtt ccatcgactg tctccaaagt cgccgtgctg acgcgcatcc
      961 tgaatcccta cgccgtgccg gccatttgtt cctgacatca tgattcaaaa cccagccaat
     1021 ctcgtccttc cgacttcgct cttcggcggc gggctggtcc ttccctacct ggatttccaa
     1081 cccgttcttg gcgagaacgt cttcatcgcg cccaacgcgt cggtcatcgg ccgcacgacg
     1141 ctcggcgacg acgtttcgat ctggttcggc gccgtgctgc gcggcgacat cgccgaagtc
     1201 cgcgtcggcc gcggcagcaa catccaggat aacagcgtgc tgcacgtcgg cgacgacgac
     1261 ccctgcatcg tcggcgaaga agtcgtcgtg ggccacaagg tcatgctgca cggctgcacg
     1321 gtcgaaaacg gctgcacgat cgggatgggc gcgatcatcc tcaacaaggc ggtgattggc
     1381 gagcgctcgg tcgtcggcgc cggcgcgctg gtcacgcagg agacgatcgt gccgccgcgc
     1441 agtctggtgc tgggctcgcc ggcccgggtc aagcgcgagt tgacggacgc ggaactgcgc
     1501 aaaatccacg cgaatttcgc ggcaaaatac gtcaaggtcg cggccaacta ccggcgcgtg
     1561 ctgggctgaa cggctgcatt gaaatccatc ggctccgccg gatcggacgg atccatccag
     1621 ccccggctac ttctccctca cccaggtctg gatccgcgcc agttccttcg cccattcctt
     1681 cgcgtcgagc cgcaattcca gaaacgcctg catcagcccc tcgatgatgt gctcgaattc
     1741 cagcatcatc tcggtgcgca gcgccatcat atcttcaaac ggcatccgcc cggggttggg
     1801 aatcgccagg cccgccaggt tgaaattctc gccgtccagc gttgcgaccc actccacgtc
     1861 ctcgcgcttg agccggatct tcgccttctt cagtttcttg ccttcgacca gcgccgtccg
     1921 cgcctcgggc gaggtcgccg gcgattcccc cttcagcccc gtcgcgcggg cgtccccata
     1981 ctgcgcatcg agcacgatcg gcccaagaat ctcggcctgg atcgcgcccc ccttcaggtt
     2041 cacccccccg cccgtctcgc tcttgaacca gagccaggtc aggaattcct tgccgagaaa
     2101 cgccttctgt tgaatcagtt cgaggacgcc catggccgtt accgcgccac cttgaccatg
     2161 gcggggcgca gcagccggtt ccccatccga tagccgtcct gaaacacctc ggtcaccgta
     2221 ttctcgggat gctccgcgtc ggccgggtcg gtcgcgaccg cctcgtgcag gttcggatcg
     2281 aacggctgac ccatcgccgg gaccttctcc agcccgttct gttcgagcac ctggctcatc
     2341 tgcttttgca ccatctgcag gccctgcagc agcgcttcca ccgattcgcc gggattcgcc
     2401 agtgcccgtt cgaaattatc cagcacgggc agcaactgtt cgatgagttt catctgggcc
     2461 tgggcgatct gctgttcctg atcgcgccgc acccgcttgc ggtaattctc gaattcggcc
     2521 tgcgcccgca ggagctgatc tttcagccgt tcgagctgct cgttctcttc gatcaccgcc
     2581 cccgggccgt ggtgccgatg gccgccatga tcgccgtggc cgtgatggtg tgaatggccg
     2641 tgttcgtcgc acagatgcgc cgccgccgcg gtttctgcct ccgtcgcccg ataaactcgc
     2701 cggcgcagac gccgggccaa ccgatgatcg acgccgtaga tccgctcgcc catgtcgcat
     2761 ccttccagtt caacaactct ggttgccgtg atgttccgtg ggttttctgg cgcaccgggc
     2821 gcccccacgc ggactgaacg ccactttagc aggcttcgcc ctgcgcattc aatccaactc
     2881 ggccgccctg cccggccttc atgatcgcct gaagtcgctt tatgtcattt atgtctcttg
     2941 cgtcccttcg ctaacttggc gagcgcgaat cacgaggatg agggacgagg acaagtgacg
     3001 aatagctgct gagatggtcg tgccaaaaac ccttcgtgcc agaaacacca tgcaaacgac
     3061 cgggccgcat gttacggatt taagggagcc tgcccgtatg agcgccgcca cgcccccgcc
     3121 accccccccc ggccgccgcg tcccggcctc ggcgacgccg cgcgcgaaac cgcccgccgc
     3181 ccgttccggt cgaccgcgcg cgaggctcaa ccggcgttcc gcctgctgga ccgcgcacct
     3241 ggcggccggt ctcggctgcg gcctcgcgct cctgttcaca gcctggttcg ccagaaccaa
     3301 ccccgcgctg atccgcctct ttcagtacct gaccgaggaa tggcatttcg gttcgggctt
     3361 tgaatggcgg atgctcggct ggaccgcagc cgcgctcttc ctgtttgtcg tcctcggccg
     3421 cttcttgctg gagtgcttcg agctctacct cccccgttcg gccacgctgg cactcagctt
     3481 tctgttcggc atgggcctgg ccggcttcgt gctcgagctg ctcgccatcc cgcactggtt
     3541 gtatcgcgcg ccgatggccg ccgcgctcgg gggattgctc ggcctgctct ggctgggcgc
     3601 gatctggtgc ggccgccgcc ggcccgccag cggactgggc ggcgagcccg gatacaacga
     3661 gacgctcctg cggcgggcgc tggcccgcgc ggcctggcgt gccgccgtga ctcgcccgcg
     3721 cggcgccgcc tggctcctgg ccgtcccgct tgtcgcgctc atcgtcgcca tccagttctt
     3781 catcttctgg cacgccgtcc tctatccgga ggtctactgg gattcattga tcctctacct
     3841 cggctacgcc cgcatgacct tcctcgaaca tggctttcac accaaggtca cggggcaggt
     3901 cggtttcggc ctgggcgcca attatccgca tctgttcccg gtgctggggg cgggcattgc
     3961 caccgccgcc ggccagtgga gcgagctccc ccagcggatc atcgccccgc tgtgcggcac
     4021 cgccaccacg ctgctggtct accacaccgg cctgcgcctg acgcgccacg tgatcttctc
     4081 gctgatcctg acgctgctct accgcgccgt cccgctcggc atcatctatg accagtatgc
     4141 gtcggattac gcgctggcgc tgctcttcgc cgccgcgttc ctctatctct gcctgctcta
     4201 ccttcaaacg gccctgccgg gctacctcgc cgcggcggcg atcctgaccg ccctggcgat
     4261 gcatctcaac tacctgatgg ggattctctg gctgccctgg gtgatcacgc tgtggggggc
     4321 tcacgccggc gcgccccggc ccgacccccg ccgcaacccc gagttggagg gcgccccgtg
     4381 gaccgccctg ccggcgcgcc cgccgctcgc gcgcgcgctg cgccggggtt ggctctgggg
     4441 catcctcctc ctgtcctgcg tgatcggttc aacctggtac attcgcaact ggatcgtcac
     4501 cggcaacccg gtctatgcct ttttccacga gtggctcggc ggcaaacaca tcaaccccga
     4561 agtcatggag gccgccgcgc acgaatggcg ggccaacggc gccggcattg gccggctggg
     4621 cgactcgccg atggaacgcc tgcgcgccgc cttcaccttc ttcgccggac ccggccccgg
     4681 cttcttctgg catcagtatt atcgcaccga gccgctcctg ctcggcttca cgctcttcgg
     4741 cgtgctggcc tggctggggc gctggatcag tggctgcctg tcgggccgca gccgccgccg
     4801 gcggcgcgcg caccgggtcc tcgcgctgcg cttcggctgg gttgtcgcct cgctgctgtt
     4861 cggactgctc gctttccact tcgtactggc gccgttctat ctctatcaga tcatcatgat
     4921 cctcccctgc gcggcgctga tggtcgcctt tgcctggccc tggtggcgcc tgccgctgtg
     4981 gcgcaccgtc ctgctgctcc tggcgctctg ggtcggcgtg atccccggcc tggccatggc
     5041 gatgatgggg ttcaaaatcg tcgggatgtt tcagctctcc gccgaccgca ccgaaagccc
     5101 gctgacgctc tatccgctgc gccaccccct gccccgcccc gaggtcttct accgctggcg
     5161 cttcggcgag gatgcccgca tgtgggacta catcaaccgt aacctgaggg gcgagcgcct
     5221 gctgactcac gacaatcgcc acctggtcta cgacccgtcg atcacgcttg tgcacctgga
     5281 cgactggacc gatgagtgga acatccaggc gctgtggggc ttgcccaacc cggccgaccg
     5341 cgtccggcag ttgatccagc tccacaatat ccgctactac ctgttcatcc cgaatgaact
     5401 ggcggccgcg accaatcgcc acatgcagac cgagcagtgg gtcgagctgc acctggccga
     5461 actgatcttc caggccggcg agaaccggct ttaccggctg cggctgccgc cggaggacca
     5521 acccctggcc gccccgacca cgccgttcgc ggccggcgcc cccgccgcgg atcaaccgac
     5581 aaccgcgacc ccgtgatggg tgcggacggc tacgggtcga tttttttacc ctatcgtgcc
     5641 tccccgccct ccgcccggac caccgctcca aggtttacct ttcagcccgg tatgctagaa
     5701 tgcccgagtt ccgattgcgt tccagcccga ggatttccca tgcccaccga cgccgaccca
     5761 gaccccgccc cttccacctc ccgctcaccg gagggaacgg agcagccgga tctgccgctt
     5821 gacgattcac cccttgacat ccagcacctg gtccagccgg cggaggccct gccgccgctg
     5881 caactggccg acctgcccga tccgctccgc caggcggcgc tccacgccgg ctggaaggag
     5941 ctgacgcccg tccagcagta tgcgatcccc tacatgctgg ccggccgcga cctgatggtc
     6001 cagtcgcgca ccggcagcgg caagaccggc gcgtttctgc tcccgatgct cgaccggatc
     6061 gacttcacca ggccgaaatg ccaggccctg gttctcgtcc cgacgcgcga actggcccag
     6121 caggtcacgc gcgaggcgca actgctcagc cagtccgccg gcctgcgcac gatcgccgtc
     6181 tacggcggcg tcggctacgg cgcccagctc gatgccttcc gcgacggcgc gcatctggtc
     6241 gtcggcaccc ccggccgcgt cctcgatcat ctcctgcgcg gctcgctcaa gctcgaccag
     6301 ctccgcttcc tcgtcttcga cgaggccgac cgcatgctcg gcatgggctt ctaccccgac
     6361 atgaaggagg tggaacgcta cctgcccgcg cgccgcgagg gctacatgtt ttccgccacg
     6421 ttcccgctca gcgtgcagcg gctggcgcac cagttcctca accagccggg cttcctcagc
     6481 ctcagccgcg acaacgtcca tgtcaccgac atggaccaca tctactacgt cgtgccgccg
     6541 atggagaagg accgctgcct ggtgcggctg ctcgagatcg aaaaccccgg cgcggcgatg
     6601 atcttctgca acacgcgcac gcgggtcaat tatgtcaccg ccgtgctgcg gcgcttcggt
     6661 tacgacgtcg accagatcac ctcggaattg acgcaggtcg cccgggagaa cgtgctggcg
     6721 cgcatcaagc ggcggcaact gcgattcctg gtcgccaccg acgtcgccgc gcgcggcatc
     6781 gacatccccc acctgacgca tgtctttctg tacgaagtgc ccgaggagac cgagctctac
     6841 attcatcgcg ccggccggac ggcgcgggcg ggcgccagcg gcgtcgccat ctcgctcgtc
     6901 tcgtcgctcg aagaactgga cctgcggcgc atcgccaccc gcttcaagat cgaactggag
     6961 aaacggccca tgccgacgga agaacagtat aacgagatcg tcgcgcagcg gctggtcgcc
     7021 ctgctcgaat cgcgcctgcg ccaccgcgac cgcctccagg tcgaacgcat gcggcggatg
     7081 ctgccgctcg cgcgcgcgct gagcgagacc gacgacgagc tcgccctgct ggcaatgctg
     7141 ctcgacgacg cctaccagga tttgctccac gcgccccccg aactgccgcc gccggagaag
     7201 gacgccgacg aaccgaaggc cggcaagcgc cgccgctcgc gttcaagagg caagggcaag
     7261 gccccagccg gacccggcga ggccgctgcc gccgagccgc aagccgcccc ctcgccgccg
     7321 cccgagcggc ggcagccgga tgccccgccc accgacgcgc ccgccgaacg caagcccggc
     7381 gcccgccggc gcgtcgtccg gcgccgcgaa cagtccgact gagcccgtag agtttttact
     7441 taatccctcc cagatctcgt ggcctcctca gcgcggcccg cggcgcgccc ggctgcccag
     7501 gaccatccgc tcgagaaact ccgccgactt gagcacatgg ttcaggtggt cctcccagac
     7561 ccggatcaac caggtgagcg cttcgcgacc cgcccgggcc ggcaactgcc aggaaccgtc
     7621 gagcggcggc ggccgccgca gcccctcccc gatcgcccgc accatctctc ccgagagcgg
     7681 catcacgtga taccggtggg cgtcgccgtc gtggcggtcg ggctcgggcc gggcgatctc
     7741 caatcggtgc gtcgcgaaat ggtggaagca ggtcgcgggc ggcgccgcgg gatccagacc
     7801 ctcccccgga tgcggcggaa aaccggcgtg atgcagcagc cgcagcagca gcgcgacgag
     7861 caccgacccc ggccccgccg catcctccag caccgtcagg aagcggcagg cttcctcgaa
     7921 ataatacggc tcgggcggcg cctcctccgc cagccggccg agcgtctcca agccgacgcc
     7981 ggcgtaagcc agccgcgcca gatcgcgacg cagatagtcc cagctcttga cgaggccgac
     8041 gccgcccgcc gtccccagct ttccgctcgc cgccggcaag cggaattccc ctctgaccca
     8101 cgcgtaggct tcgaacgccg cctgcacctt tcccttgcgg cgcgcgccgc gggcgagcac
     8161 ccggatcacc ccttcgctcc gcgtcagcag gctgacgatc agcgtcgttt cgcgatacgg
     8221 cgacgtggcg agcacaatgg cgtcaagcgc gggcatgggg gccgttcaca aaaggcgggt
     8281 ggcagggagc ggggtcgagg ccgcttttcg gaaagttgcg ccgtcctgtt ggatgcggca
     8341 cccgcgccgc gtccttcacg tcctttacgt ccctgccgtt agtttcaccg gacgccctgg
     8401 ggcgcgcgcg ggggctcgaa ttcgcgcagt tcgggcgtcg tgaaccggaa cgtgtagggc
     8461 gcgttgtcca gcttgtcgcg atcgaacgtc tcggcccgcc gaccaatcgt gatcgaatac
     8521 tcggtatcct cttcccactg cgcctggatg ttcagaatcg accagccggt tgcccgatcc
     8581 tcctgaacgg agagtaccgg cgccatgcgc agccgcggcc ggaaccgcag cgtgtccgac
     8641 ccgagcgagg cgggatccat ccgcgtgttg aagaaaacgg agaccggctc gctgggcgac
     8701 aacgagcgct tgagcccctc gtcggccggg accgtgcgga tgaccgccgg gccggcggtt
     8761 gaaaactccg cctcgaacgg ttcctgcagg gtcagcccct cgacgtcctt cgccgtgacg
     8821 ccgatcgtca tccggtagcg ctgattgagc agcgcgacgg gatcgccctc gctccccgcc
     8881 agctcgatct ggacgaaatc ggtgtcgggg ccggccttgc cgccgcctgc gaacacctca
     8941 aaactcaccg gcggatcgaa cagaatcgcc cggcgcagcg atgggagatc catcttcttg
     9001 ctgaaccgca cccgcaccgg caccacttcg cgcaccagca cgccgcgcgt tccgtcggcc
     9061 gggctgaccg acagcacctc cggatagtcc cggatcggct cgaggatcat ctcctccagg
     9121 ttcacctcct cgcccgcggc cagctcgacc ggaccgagcc gcgccggctc gtatccgtcg
     9181 gcctgggcga tcacgtcgta aatcccgggc tcgatctcgt cgatcacgta ttccccctgc
     9241 gcgttggtcg tggccgcgtg cgacgtcccg atcagcgcca cctggatgcc gctcatgtcg
     9301 cggatttcat tatcgttctt gcgcgcaatg ccgcgaatca cggcgcccgc cggctcgatc
     9361 cccggttcgg cgcgcagttc cagatgcacg tcgacggccg gggcattggt caggtccacc
     9421 tccaccggcc gcgcatcctc gaagccgatg ccgctggcca tgacgacgta gcgccccggc
     9481 gccaggctcg ggaattcaaa cgtcccatcg ttctccagga tcgctttcag ttcgggatgg
     9541 ttggcgatct caatgaggac ctggtcgaat gcgttgctcg gttcgccgtc ggggccggtc
     9601 agcgtgacga cgccgcggat ccgccggtca tggcccgggc cgcccccgcc ataatttccc
     9661 gccggcgtca gctcgatcac gagccgctcg tcgggctcgc cgggcagtac gcgcacgctc
     9721 tgcgtccacg tctggaagcc gggcatcgtc gcgcgcaact ggtagcggtc gggcggcagg
     9781 ttccacagca ggaaactgcc gtcggagtcg caggtggtcc ggtagggggt attctccagc
     9841 gagatggtgc aggcggccca gttgatcgtc tcgccggcca tctcgtcgcc ggccaccacg
     9901 tcgccggcga tggcgcccag agcggccccg gccggctcgg cggcggccgt ctcgggctgc
     9961 aggaccagtg gatcgagcgg aatcgtccgc cccgcgtcgg ccggcgtcac ctgcaccgga
    10021 ccgagcttcg ccccatgaaa gccggcggcg cgggcctgca gctcatacag cccctcgccg
    10081 acgggactca gctcaaagcg gccggcggca tcggtcatcg ccaccagact ggagccgggg
    10141 atgaagacct gaacgccact tgaatcgtcg cgtccctcca gttgcacgac tcccgaaatg
    10201 cggacggtcg cggggccgga ctctcccccg gccgccgcgt cccccccacc gcggcgctcg
    10261 gcgcgccccc ccgcgtcgca cgcgaccgtg agcagcagca ccacctggac cagcagggcc
    10321 ggccacgatc tggttcgggc acgatccatc cccggcgtcg acggcctttc gcgcgggcgc
    10381 tattcggtga tcaggtgctc cacctgggcc tgttcgagat attcccgttc caccgcctcg
    10441 ccatactccc ggaacagttg gggcaggagc cggttttcga cctgcgatgc cacggcctca
    10501 tactccggaa tcccggccgg ctggcgcttc tcgaccagca ccgacagcac gcgctgatcc
    10561 cccacgacga agggctggga gaactcgccc tcctgcagtt ccgcgatctg ggccgcgatt
    10621 ggctcctcga tgcgatcgac cggcaccgga tcggccgcct ggggaattcg gacgccctgt
    10681 tgcagcagcg tctcattaac ctgaccggcc agattgatga aatcctcggc cgatttcacg
    10741 tccgccaggt cggccgtcat catttctttc agttgatcga ccgccgcctg gcgcgcctcg
    10801 ccctccagtg cctgttgttc gggcgacagg cgccgctcca tcgtgctgta ggccaccatc
    10861 tccggcgttg cgaattcctc gcgatgctcg gcgaaatagt tccgtttcat ctcgtccgtg
    10921 atcgcgtcca tggcccgcag acgcgccaca tgattgatct tgttgctccc caggaagccg
    10981 acctccatcc attccagctg gcgtgccagc gtgctggcgg gatcgtcata gaacgggcgc
    11041 atctcctcca cgatcaggtt gatctgcaac ttccccatct cgcgcaggaa gccgcgcacc
    11101 ttctctcgat cgatcggcgg cgacacttgc gaccacccct gcgcgatctc gccgtagtag
    11161 atcttgcgat cgccgacgct ggccaccacg gtgtcggtgg acggctcggt ccggcgaacc
    11221 accgtcatcg tgtcggtcgt cgcatcggcg tcggtcgtca gcgcatcgta attgatttcc
    11281 agggccgggt tcagcatcaa ccgatcgagg agctcctgaa tcagctcgtc gcgcttctcc
    11341 tgcttgagcc gcatctcgat cgtcgagcgg acatcttcca gcggccggaa gccctcctcg
    11401 cgcttgtcga ccacttcgat cacctgccag ccgtgtttgg ttcggaagat cgggctgatt
    11461 tcgcccaccg gggtttggcg ccccatctcc agcaggtcgg gcagcatgtc gcgcgtcccc
    11521 gccggcagcc agccgatcac ctggccgcgg gcctccggct ccgtctcgga atactgcttg
    11581 gccagttcct caaacgtctt gccttcgtcc agttccttga ggatctcctc caggcgctgg
    11641 cgaacctgtt gcgcgtcggc ctcatccatc ggcaccagca gttgctcgcc cggcaccagc
    11701 ggcttgaata cttccccttc ctcgcggcgg gcgggcttgc ccgcaatgtc ggcccggatg
    11761 ttgtccgcgt tggccgcatc gccgctgatc ttttccgcga tcgattcgag cgtgtcccct
    11821 tccgccacga tgtacggttc atacgtcagc aggatcaggt ggcgcatttt gaagccgaaa
    11881 ttctgcttga actccggctt gtgctcctcg taataggccg tcaccaccgc ttcggggggt
    11941 tcaaccagct tcgcctccaa ctccgtttcc acgaaccggt tgacgatcaa ctggtcgcgc
    12001 gtccgctccc actgttcaac catcggatta taatagacca gctcggggtg gttgcgcagg
    12061 tcgtgcatcg ccgcctggcg cgccgagata tggtcgacca ggcgcatgat gaggtcgccc
    12121 ggcagcgccg cgatctgatt cagcgacagg ttgcccctca gccacgggac ggggttggcc
    12181 gagccgtaat ccatcagatt gcggatcgtc agctcgcccc acgcattgcg cgccgcgacg
    12241 gcttcatccg gccgcggttc ctccagaaac tggattgact ccgtattcag ggtatcggtc
    12301 gtggcctcgc ccatgggccc catgggctgg gcgtatccgg ccgggatcgc gaccagaagc
    12361 atcacaagca gcagggcaaa acgtttcatg agagcggtcc ttcacagggg tcaagtgttc
    12421 gattgccgcg cgggagcgcc gtggcgttcc ccgtgcagtt gcgtgaaagc ctgaggccat
    12481 tcacattatc cattccctcc gcggccattc aagacccatt tggccgacgc gccgttgacg
    12541 cccgccatcg gctggcctaa actgggcgcc gtcttcttcc ccgcgggcga tcccttttcc
    12601 gggccgcgac ggcccgccac gcccatgccc ccgaccccct cccgcagctt ccatgtcctg
    12661 gtcgtgctct ataacagcgc gcagtggatc gacggctgcc tgcgttcggc cctggcccag
    12721 acgctccccc ccgcccgcat caccgtggtc gacaacggct cgagcgacgg cggaccggag
    12781 ttggccgaac ggatcggcga tccccggatc cacgtcatcc gcgccgggaa aaatctgggg
    12841 tttgccggcg gcaacaatcg cgccgccgcc gccgcccgcg acatggattt cctcctcctg
    12901 ctcaatcccg acgccgtgat ggccgccgac acgctgcagc ggctggccga cgcgttccag
    12961 accgaccccg ccatcggcgt cctcggctgc aaggtcctcg agcccgacgt cgccacgatt
    13021 cagcacgtcg gcatccacct ccgcggcaac ggcctcccgt ccaacctcgg ccagggcgaa
    13081 cccgacaacg gccagtacag cggcatccac gatgtggcca gcgtcctggg cgccgcgatg
    13141 gccgtccgcg ccgacacctg gtgcgagctg ggcggactgg atgaagcctt ctggcccggc
    13201 tactacgagg agaccgactt ctgcttccgc gcccgccgcg ccggctggcg cgtcgccgtc
    13261 gcccgcgacg ccgtcgtcac ccactttgac gcctccgccg accgccgccg cgatcccgaa
    13321 ttcctccgcc tcttcttccg caatcgcgcc cgctttctgc tcaaacacta ccgtgcgcgc
    13381 gactggctct tccgctatct gcccgacgaa acgcgctggc tgacctactg gggcagccgg
    13441 cagatgcgcc gcatcgccct gcgcagcctg tggcaagtgc gcgccgagat gcggcgcgaa
    13501 cgcaagctcg ccgctcgccg gtggtcgccc tgatgctcca tgtggtcctc tacgccccgc
    13561 gcatcccgca gaataccggc cagatcgccc gcgcctgctt tgccatgggg tgccggctgc
    13621 acctgatccg gcctctcggc tttcgcctgg acgaggcttc gctcaagcgc gccgccgtcg
    13681 gccacctgaa ggcgaccgat ctgcggctcc atcgcgacgg cgacgccttc tggtcgtccc
    13741 ccgatctcga tcccgaccgt tgctggctcg tcaccaaaca tgggtcggtc gactacgccc
    13801 aggtgcaata ccggcagggg gactggctga tcttcggcaa cgaaaccgaa gggcttccgc
    13861 ccgactggct cgagcggcgc cgcgaccgga ccgttgtcat cccgatgcac gaaccgcgcg
    13921 tccgctgcct caatctggcg acggccgccg ccgccgtcct gttcgaggcc ctccgccagc
    13981 tgcgcctgac gcaagagcat ggctgatccc gccgccaccc gcccgcgcgt cgacgcgatc
    14041 atcgtcaatt accatcagcc cggattgacg caggcggcga tccgctcgtt tctcgattcg
    14101 gatgggatcg acccgcgcgt gatcctgatc gagaacgagg gcgacggcgc ctgggccggc
    14161 gacttgctcg cgcgggaacc ccgcatcgag ctgatcgcga accgcgccaa cgtcggcttc
    14221 ggcgccgcct gcaaccaggg gttcgcggcc gtgctgcgcg gcgacgccca attcgtgctg
    14281 ctcctcaaca acgacgcggc ggccgcgacg gccgacgccg tcctccgcct gatcgacccc
    14341 gcggcgcgcg cgggcctggc cgccccgcgg atcctgctgc ccgatggccg gatctacgcc
    14401 gccggcggcc gcgtcgagcc gtggcgcgcc cgctgccgca accgcggctt ccacgaaccg
    14461 gacgctggcc agtacgaccg gccgaacgag acgccgttcg cttccgcctg cgccctgctc
    14521 attcaccgcc gcgtcctcga ggccggcctg cgcttccacg cgccttattt cctctactac
    14581 gaggacgccg atttctgtct ggccgcccgc cgcgccggtt ttcgcatccg ctatgtcccc
    14641 gaatcccggt tcattcatca cgagtcggcg tccacggcca cgccgttgcg accccgcctg
    14701 ttgttttaca cgacgcgcaa ccggctgctc ttcctgcgcc gtaattacgg ctggccgatc
    14761 cggctcgtcg gcggcgtcta cctggccggc gtcgtcgccg tccgccagat cgtcggaccc
    14821 ctgcgcggcc gcggcgatgc cgcccgggcg acggccagag gctatctcgc cggtttggga
    14881 ttgctttccg gagcggataa atagctttca acgccgggct cggcgcgcgc ggccgcctac
    14941 ttcggcatca tcgagccggt ctcggcaaag cggatgtgga aatcgaaggc gtggcgcagg
    15001 tcgtgcgcga tatggccctt ggcggcccgg ttgatgtagt cgtgcagcag cggcttgtag
    15061 tcggggtgcg cgcagttgtc gatgatgatc cgcggccggt ccgccggggc cacgccgcgc
    15121 agatcggcaa atccctgctc ggtcaccagc acctgcaccg aatgctcgtt gtggtcgctg
    15181 tggctgacca tcggcaccac cgccgagatc gccccgtcct tggccaccga ggggcaggta
    15241 aacatcgaga tgtaggcgtt gcgcgtgaaa tcgcccgaac ccccgatgcc gttcatcatc
    15301 tgcgtgccgc cgacatgcgt tgaattcacg ttgccgtaga ggtccacctc cagcgccgtg
    15361 ttcatcgaga tcacgccgag acggcggatg atctccgggt tgttcgacat gctttgcgga
    15421 cggatcacca gccggtcggc gtagcgatcg aactccccgt agagccggtg cagcgcatcc
    15481 gggctgagcg tcagcgagca ggtcgacgcc cccagcagcc ggtcggcgtc catcagttgc
    15541 agacaggcgt cctgaaacac ttcggtatac atgtagaacg gcggaatgcg gctatccagg
    15601 ccgatgttgg ccagcaccgc gttggccacg ttgcccacgc ccgactggat tggcaaaaac
    15661 tcggggggga tccgccccgc cttcatctcg cgcgccagaa actccacgac attgcggccg
    15721 atctgctcgt ggatctcggt gtacgggtcg agcgaccccg actcgtccgg ctcgtcggtc
    15781 tccacgatgc cgatcacttt ttttgggtcc accatcgcgt acggcgtgcc gattttatcc
    15841 agcggatgga agatcgggat ctcgcgccgg cgcggcggca gggcgggcac gaagatgtcg
    15901 tgcatctcgc gcagccgcgg cagatggaac cggttgagtt caataatcac gcggcgcgcg
    15961 cgtttgagaa acgtcggtga ggcgcccccc gccgtcgtca gataaatgtg cccctcgcgg
    16021 ctgatcgacg tcgcctcgat gatcgccacg tcgatctcgc cgaaaaagcc atactcgaca
    16081 tactgtggca cgtgcgacag gtggatgtcg aagaacggga cctgatcctt gttgatccgc
    16141 tggcgcagcg tgcggtcgct ctgatacggc gcgcggaacg cgatcgcatc ggcctcggcc
    16201 agcgccttgt ccaccgacgg gccggtcgac gccccggtca gcagccggat cttgaagggc
    16261 cggccggctt cgtgttcagc ccgggcgcgg ttggccagcg cgcggccgca ggccttgatc
    16321 gtgccggcgg ccgtaaaccc gctggtgaca accgtgtcgc cgtgttgaat gtgagtgacc
    16381 gcttcatccg cggtcaggcg gggcaattcg gcgcagctca tgggagaacc tcgcttcgct
    16441 acaagccgcg tccaagttcg gacgcaaagc gtaccagaac ctagcatggc atttatggat
    16501 gatcaagtct tttgaatcac gctgccacgc ggacccctca tcggtccgca tctctcgtcc
    16561 ccgtgctcgc tctcatcggc cgtctccaaa attcagatct cgtccctcgt ccgcggctct
    16621 cgaaaaccgg ctcttctgtc ccctcgttgt gaaccgccgg tttctgcgca actcgaaaga
    16681 aaaaagcccg gcgcggtcgg gccgctccgg gcttttgcca gtttcaccgg cgaatcggtc
    16741 aatcatcctc gtgcttctgg cgcggacgtt cgcggaagcc gtattccttt ggctcggcgc
    16801 tgctctcgcg ccgttcgcgt ccgccccgct cgccgccgcc gcgcggaccc cgctcgcggt
    16861 cgccccggcc gccgcgctcg ccgccgcggc cacggtcgcc gccgcgtccg ccgccgccgc
    16921 cccgctcgcc gccgcgacca ccaccgccgc cgccgccgcg ccgacccccg tcgtcgggcg
    16981 tatactccag accctgctct tccatcagga tcgccttgcg cgaaagccgc accttgccgg
    17041 ccggatcgat attgaccacc ttgaccttca ggctgtcgcc gacctgacag acgtcctcga
    17101 cgcggtccac gcggccgatc tccagctcgc tgatgtgaac catcccgtcg cggcccggca
    17161 gcagctcgac aaacgcgccg aaatcggtca cgcgcaccac cttgccctca tacacctggc
    17221 cgacggtcac ttcggccgtc atctcctgga tcaactggcg cgcggcttcc atcgactggc
    17281 cgttgggcga ggagacgaaa atcttgccgt cgtcctggat gtcgatcttg gcgccggtcc
    17341 gttcctccag cccgcggatc gtcttcccac ccggcccgat caccatgccg atcttgtcga
    17401 ccggaatctg gatcgaggag acgcgcgggg cggccttggc cagctcggcg cggggctccg
    17461 gcagcagttc ggtcatgata tcgaggatct tcagccgggc ggccttggcc tgctccagcg
    17521 cctgctccat gatctcgaac gtgaccccct cgatcttgat gtccatctgc agggcggtga
    17581 tgccgtcgcg cgtcccggcc accttgaagt ccatgtcgcc caggtgatcc tcgaccccct
    17641 ggatgtcggt caggacggcc atcttgcctt cctcgttggc gaccaggccc atcgcgatcc
    17701 ccgccaccgg ctgcttgagc ggcacgccgg catccatcag cgccagcgag ccgacgcaca
    17761 ccgacgccat cgaactggac ccattggatt caaggatttc cgagacgagc cggatcgtgt
    17821 aggggaaatc ctcggtcttc gggatcaccg gctgcagcgc gcgctcggcc agcgcgccat
    17881 ggccgatctc gcggcggccg ggcgcccgca tcggcttggc ttcccccaca ctgaacggcg
    17941 ggaagttgta gtgcagcatg aagtacttgt catacgtccc gagcatcgtg tcgatcatct
    18001 gggcgtcgcc gaccgtcccc agcgtcacaa cgccgagcgc ctgggtctgg ccgcgcgtga
    18061 aaagcgccga gccgtggacg gccggcagga tcggcgcgtc gcaggtgacc ggccggattt
    18121 catcccgcgc gcgcccatcg acgcgctggc cttcctcgat gatcagcttg cgcaccgcct
    18181 ggctgagcag ctcggtcgcg tagtccttga cctggccgac cagctcggga tgctccacgg
    18241 cgatggcggc cgccgtctcg gcgataatcg tgtgctctcc ctcgctgaac gcttccttgc
    18301 tcagccggcc gtggaccagc ttgtcgaagc gttcgacgca cagctcgcgg acgtgcgccg
    18361 cgacctccgc cggcagcggc tcgaccttga tctcgaactt cggcttgccg tgctgggccg
    18421 caaactcgtc gatgtcttcg atgatctcgg tgatcgcttc gtgggcgaag cgcagcgcgg
    18481 cgatcatgtc ctcctcgctg acgaattcgg cccccgcttc gaccatgttg atcgccttgc
    18541 gcgtgccggc cacgatcagg tcgatatcgc tcaccttctg ttgctcgaac gtcggattga
    18601 gaacgaattc gccgtcgacg cggccgacgc gcactgccgc ggtggccgtc gggaacggca
    18661 ccgacgacaa gcgcagcgcc aggctcgtgg cgttgagcgc cgcgatctcg gccgggtgaa
    18721 tgttgtcgct cgacaggatg gtgatgtaga ccatcacctc gttgcgcaga ttttcgggaa
    18781 acagcggccg gatggcgcgg tcggtgatgc gcgcgcggag ggtttccagg tcccccggcc
    18841 ggctttcccg cttgaagaag ccgccgggga tccggccgcc ggcgtagaat ttctcccggt
    18901 aatcgaccat cagggggaag aaatcgcgcc cttcgagcgg ttcgggtgag acggtgacgg
    18961 ccaccagcat caccgtgccg cccacctggg cgatcacggc cgcatcggcc tgtttggcca
    19021 tgcgaccgac ttcgagaatc agcggttccg ctccaaaggg aaccgagatg cgctccatgg
    19081 acatgaagca ctcctttcga tgcggcacgg cgcggcggcg ggggccatcg ccggtccggc
    19141 gggcgcggag ccgaccggtc ggcgggccgg ggcgggagga aggtcatcgg ggttttaaac
    19201 acaagggtcg gcgcggcgtc gcctgcgcct gctcgactct tctgtttaaa atcccgggtt
    19261 cccttctccc cgtggcccgg cgcggcgcaa atcgcctggc cgcgtgcgtg ccgtcgtgtg
    19321 gtttgggggc ggcagagaat agccgcgccc ggggaccctc aatcctccgg tcgccggcgg
    19381 cccgcgggcc actgcggcga ccgaccggcg tcagcggcgc aggccgagac gcttgatcaa
    19441 ttcgcggtag cgttcgatct ggttgtcggc cagatacgtc agcagcgcct tgcgcttgga
    19501 gaccagccgg agcaggccgc ggcgcgaatg atgatccttg ccgtgggcct tgaagtgctc
    19561 cgtcaggttg ttgatgcgct cggtcagaat cgccacctga acttccggcg agccggtgtc
    19621 gttgtcgtgg gtcttatact gttcgatgag ctgctgcttg cgttcgagcg tgatggacat
    19681 gagggtctgc ctccttggat gacaggcggt ccagggggga cgacgccgga agatcggggc
    19741 gcggcccgaa ggccggagcc cggcccaagc gcccagtcgg cggtgggtca aggagttgcc
    19801 caccggctga ccgagtcgcc cggctcttcc gcctgctggc gtcaggttgc ctggtccggg
    19861 gaccaaaccg acctattgaa ccggaaggaa cgaacccgtg tcaacgtcgg aatttgacgc
    19921 aacgggagcg aaagggggag gaacccgcct gcgcagcctg gccgcaccca aaacaaactg
    19981 cccctgaatt atcgtgtcgg gatcgctatc gggatcaaat caatcgcccg ccgcattccg
    20041 cggggacgtc ggaatcgttc ctcacccgaa tcggctctcg gctctcgtcc ttcgtccccg
    20101 gctctcgaaa tcacaccgca attcaagttg acgccgtagc cactgtatga tatatatgtg
    20161 cacagttggc cggcaaatgc acctgactct cgaccaccat agcggcgtcc cgatctcgca
    20221 gcagatcacc gggcagatca aatatctggt cgtgagcgga ggactggtcc ccggtgagcg
    20281 catcccctcg gttcgggcgc tggcgacccg gctccgcctc aacccgacaa ccgtggcgcg
    20341 cgtctaccgc caactggagg ccgagggcgt gatcttcacg catccggggc gcggggcgtt
    20401 catcgcgggc cggccgcccg gtctgacgca ggccgaacag caccggcggc tggaaggcga
    20461 tatccggcgc ctgctcgtcg aagcgggccg cctgggcgtc agcttcgccg atctgctgcg
    20521 ctggatcgag gaggagatcg gccagatcga gcagctccgc gatgcccgct gaccggcgct
    20581 ggcccagcgt ggcaggatag tcgaaagaag gaacaagcga ttccctgggg acggcacaca
    20641 accaccccag cgttgaccga cgcgaaacgg attctcgaag gggagaggtg ctggcgatga
    20701 atgtcattga ggcttacggc ctgacccgct actaccggac gcagcggggc gtgcgcgatc
    20761 tggacctcgc cgttcccgag gggtgcgtgc tggggttgct cggcgagaac gggtgcggca
    20821 agacgacgac gatcaagctg gccgtcggcg cgctctccca gtggctcttg aaggatcgct
    20881 ccaattcgcg ccgggccatt caggagattg gcgttccccg taagaatcag gatcggcatg
    20941 gccccatgtc ccgcggcgtt tccgccagca agtcgtgttg gcagccgaaa gatttcgata
    21001 taaactgacg ccataatcgg ttgtccggcg cggcgcgttg cccgaccccc gcttccgcgc
    21061 cccgtttggt ggtttggctc gcattttgcc ctgcctcaga ccgcgggcgt cagggccgtt
    21121 cccccctctt ttccgcattt gctttttggg ggagcgcccg acaagatggt actggaaacg
    21181 gaaggatttc cgccgtgatc ttcgaatcgg tgcttgaagc tgaaaagacg atggtcgcca
    21241 tgcctgcccg cgccatccgc acgtttgtct gggacttcgc cgacgaaggg gtccacgagg
    21301 tcctgggccg cctggccgat tccggcgtca acggcgtcca cctcgcgctg gcctgtcacg
    21361 gcggccgcta ttactgcccg cacaatccca agcgggcgct ggttcatgcg cccgacggcg
    21421 cgctctattt ccagccgctc ctttcctgct acgacgagat caagccgcgc gtgcatccgg
    21481 aattcggcag cggggcgttc gtcgcccgcc tgatcgaatc gatgcgcgaa tacgacatga
    21541 tcgcgaccgc atgggtcacg ctgttcaata accagtcgct cagcctggcc tttccggaat
    21601 gcacgatggt caatgcgctg ggcgaccggc tggaagggac gctttgcccg gcgcatccgg
    21661 ccgtccgcgg ctacgcccag gcgttggtcg aggacctcgc gcaccgcgtc ggggtcgaca
    21721 tgatcgagct gcaggatttc gccttcccca gccacgacca gttcatcggc gcgcgctggg
    21781 tcgacgtgcc cgtgggccag ggcctcggct acctgctcag cctctgcttc tgcgagcact
    21841 gccgccgccg cgccgaggag ctgaacctgg aagtcgacga tctggcgcaa cgcgtcgaac
    21901 ggatgctgca ccgcgcgctg gcgggcgacc tgagcgagcg gcgcctcaac gacgagatcg
    21961 ccgaccccta caattcgctg gcgcgctatg ccgaggctcg cgccgggacg atcacgacgc
    22021 tcgtggatga actgaacgac gcgaccgacg gcagccacgc cggcctgcaa ctggtgctgg
    22081 ccgaggagcc cgacgacgcc tggcgctggg ggatcgaact ccacacgctg cgccagcgcc
    22141 ggctgcgggc gacgttgcag ccgggtcggg gcccggcgcc ccggccgacg ttcgtcagcc
    22201 gctatgccga ggtcttgcag ctgggccgcg acctggcggt cgacatcccg ctgggcgtgc
    22261 cccacaccga cgattactcg ccggccggcg cgatccaggc cgcgctcgag tcgggcatcg
    22321 atcatttcgt cttttcgcac tacggcctcg ttccgctcga catgctcgac tggatcgatg
    22381 cgctcgcgcg ccgttgatgg atcccgatga tttcatcccg ccgccgccgc gcgtcgcggt
    22441 tgtgatcgtg gccgcggggc gcggcgagcg gctgggcgag ctgaccccga agcaattcct
    22501 gccgctggcc ggccggccgg tcttttccta ctcgctgcgc ttttttggcg gcctcccgtt
    22561 cgtcgtggag atcgcgctcg tccacccgcc cgccggtctg cccgaggcgg cgcgcgcgca
    22621 cgtcgagggg ccggcggtgc cgctccggct cgtcgcgggc ggcgcgcggc gccaggattc
    22681 cgttgcggcg gggctgcgcg ccctgcgcgc gccgttcgac gtggcgctcg ttcacgacgc
    22741 cgcgcgtccc ttccccgacc gccgggcgac ccgcctgctg gcgcaacgcg cgatggagac
    22801 cggcggcggc ctggttgcgc tgccctgccc cgacaccgtc aagcgcgcgg gcaacgacgg
    22861 ctgtgtcgac gccacgctcg accgccggac gatctggttg gcgcagaccc cccaggcgat
    22921 tcgggccgat tacctcggct cggcgctggc gctgatggat tccgcggaag actggaccga
    22981 cgaagccgcg gtcttggagc ggctggggct gcgggtggcc ctcgtggcgg gctcgtcggc
    23041 gaatttcaag atcaccacgc gcgaggactg gctgcgcgcc gaggcgctgg ccgaccgcga
    23101 agccctggcg cggcccggcc tcaatcaacc gaaatagccg caaaccgaca aggactcacg
    23161 gagaatcggt ccgcattgca atcggtatcg gtatcgggtc ccggtatcgc atgcgcaatc
    23221 aggatcaaaa aacaccgccc ctcccgcggg catcccggca tcatccggcg aataagcaaa
    23281 cgggatcggt ctactcctcg gcgccttcgg tgccgaggcc cagcaccttc ctgcgggcgc
    23341 gctggatccg atcgagataa tagcgcgccg ccacgcgcgc ctggcggccg aacgaggcgt
    23401 cgtcggccgc gtagatgatc gagcgcgaca cgttgaacag cagatgatgc ggcggcgcgt
    23461 cgggcgcggc cgccagcgtc gcctccagat cgcccccctg cgcccccaga ccggggatga
    23521 gcagggggat ctcgggcgcg atcgcgcgaa tcgaagtgag gaactgcggc ttggtcgcgc
    23581 cgacgaccat cccacagttg ccgcgcgtgt tccattcgcg cacgcgccgc gccacgcgca
    23641 gatacaggtc gtcggccagt tgaaaatcaa acgcgccctg gttcgaggtc aggcagagaa
    23701 catacgtccc gttgcgctcg aattccagga acggctcgac ggaatcgtaa cccatgtagg
    23761 gattgacggt gacggcgccg gcgcgcaacc ggtcgaacag cgagcgggcg tacatccggg
    23821 cggtcgagcc gatgtccccg cgtttggcgt ccaggatcac gggcacatcc tgcggcacgg
    23881 cgcgcagcac ctgatcgagg acatgccagc cctccacgcc gatcgcctcg aagaacgcga
    23941 ggttcggctt gaaggcggcg gcgtgatcgg cggtcgcttc gatgatttcg acgcagaagc
    24001 ggtagatcgc ctccggaccg gagcccagat ggcgcggcag gcgcgtcggg tccgggtcga
    24061 ggccgatgca gaggctggcg ccggtctggt cgcagcgcgc ggcgaggcgt tcaaagaagg
    24121 tgcgcagttc catggagtgc agcaggcgcg gccggccggt gcggccgtcg ggacggccgg
    24181 ggaccggtcg cgagaattcg ggcggggcgg tgccgcgggc atgcagccct cgcccgcgcg
    24241 ctccccgtcg atactgtaac cgctcgcgcg gcgaccggca atgcccacgg cgcaagcggc
    24301 cgaggcgccg tcacgccgga ggcttcggct cgggcggcgc cgggctcaga accgactcga
    24361 tcacgcgcca caggtcgccg atcccttcgc cggtcgcggc cgagacgggg acgagggccg
    24421 agccgtcggc caggcccagc gcccgctgca gcgcgtggag ccgccgcgac cgctcgccgc
    24481 gcttgacctt gtcgcacttt gtcacggcga acagatacgg ccgctcgccg tgctccagcc
    24541 actggatcat ctgcaagtcc tcggggctgg ggtcgcgccg cacgtccagg atcagcacga
    24601 ccagccggat gccctcggcc ttgcgcagga agcgctccat catgggtccc caccgggccc
    24661 gttcggtctt gctgaccttg gcgtagccgt agccgggcag atcgaccagg taatagcggt
    24721 cgtcgatgcc gaaatactgg atttcgcggg tgcggccggg tgtgctcgac gtgcgcgcca
    24781 ggcccttgcg gttggtcagc ttgttgatca gcgagctttt gccgacgttg gagcggccgg
    24841 caaagacgac cgccggccgc ggcggcggcg cctggctggg gtcggtgatc gtcttgacaa
    24901 actcggcctt catcccgggg cagtctatcg ggtcgcggcg cgggcgacaa ccctccatcc
    24961 cttctgcgtc tacgttcgct ttgacgatta ccgagctatc tggtcaactg atcggcgatt
    25021 atggcggcgg ccgagacagc gtggccggcc cgcccgatcc ccgtcgcggc ggtcggtcat
    25081 gtccagcgcg cagcgtccga ttctggttca gttcggcgcc ggcaatatcg gccggtcgct
    25141 tgtcggccag ctgttctccg cctcaggtta cgaggtcgtc ttcatcgatg ccgtgccggc
    25201 catcatcgag gcgctcaacc gcgagcggcg ctacaaggtc gtgacaaaag gcaagacgct
    25261 gccgggccag tcgccggaga ccctggtgga gcacgtccgg gggatcctgg cgacggagac
    25321 cgaagcggtg gccgaggccg tcgcgcgcgc cgacgtgatc gggacggccg tggggccggc
    25381 ggttctgccg cgcatcatgc cggcaatcgc ggcgggcctc cggcgccgcg agcgcccaat
    25441 ctcgatcatc ctctgcgaga acctgcgcgg cgcggcgggc atcgcgcgcg cccacctggc
    25501 ggcggccctc cccgccgatt tcgacctggc tggtcgggtc ggcctggtgg aaaccagcat
    25561 cggcaagatg gtgccgatca tgccgaccga ggcgcgccgg cgcgaccccc tggaggtctg
    25621 ggccgaagcc tacaacacga tcatcgccga ccgcgagggc ttcatcggcg aaccgccggc
    25681 cgtcgccggc ctggtctgca agcgcaattt cgcgccgtgg gtcgatcgca agctcttcat
    25741 ccacaatctg ggccacgccg ccgcggccta tcacgggttc ctgcgcggcc acacttatgt
    25801 gtgggagtgc gcgcgcgacg cggaggtccg tcggctggtg cagggggcga tgcgcgaatc
    25861 ggccgaggcg ctggtgcggg cgcacccgag tgaattcacg cgcgacgacc aggcggccca
    25921 tatcgaggat ctgctggagc ggttcggaaa cgaggcgctg ggcgacacgg tcttccgggt
    25981 cgggcgggac ctgccgcgca agctggcccc cgacgaccgc tgcatcggcg ccctgcggct
    26041 gtgtcagcgc gagggggtgg agccggcggc gatcgtgggg gtgatcgcgg cggcgctgca
    26101 cttcaaggcg cgcgacgagc acggtgaact gttccccgcc gacgcggcat ttcacgcgcg
    26161 gctggcgagc gccggtcctg agacggtgct gcgcgaggtc gcaggcctgg tcgccgcggc
    26221 cgatgcgcgg atcgtccgag aaattctgga ccagtcgcgg cgaatggcgt ccgcgggaaa
    26281 cgagtcggcc tgaaaccgga ttggcccggg ggcggcggcc ggcctgtgcc gcggccgctc
    26341 cgcgcgctga atccgtgccg gcgggaccgc cggcaaatca cgacgcggcg ccggccggag
    26401 gtggccccct ggggggggcg tgaacgcatg cacaaacccg acaacccacc gtccgacgcc
    26461 tcgccggccg ccgagaagcg cccccccacc ctgatgcgcc attacgtgcg ctacctcaac
    26521 gcgaatatcc tgacgatcgt cgcggggttc atttcctacc ccgtgatggt ccgcctgctg
    26581 ccgaccgagg aatacggcct gttcggctac tttcagggct tcattctggt ctggatcgcg
    26641 gtgctcaagc tggggatgca gcactccatc cggcggctct atccttcgca ctgtctgggc
    26701 cggagcatcg cggagcagaa aacgttctat acgacgctgg tgctggcgcc ggcggcgctg
    26761 tcgctggtgc tgtttgcgct cacgctggcg gcgctccatg ccgtgtcgct cttccggccg
    26821 atcgccgacc tgcgctacct ggagatcatc cttgtgatca gccagatcgg cgtgattcag
    26881 agcctgatcg acaatgtgat tcgggcgcgc gaggaatccc ggctcgccgc ctggctgatc
    26941 atcgtggggc gctacctgga actggtcttc gtcgtcctgg tgatcgtgtt tctgctgcgt
    27001 tcggccatgg gggtctactg ggcccggctg gccgccgtcg gcctcatctt tttctatgtg
    27061 gtcggctggg cccggcgtaa ctgccatttc gatccgcgat cgttctcggg cgatctcttc
    27121 cgccaaagcc tgcggttcgg gctgccgctc gtcgccagcg aaatttcgtt catcctgctg
    27181 gccttcgcgg atcacgtgat gctgcgttat taccacggca acacggcggt gggcgtttac
    27241 agcatcggct atgggctggc gatcacggtc ggggtcgtgc tgcgatttcc gctggagccg
    27301 gcgttccagc cggtggccaa ccggctctac gagaccaagg ggatccagcc ggtgctcggc
    27361 ttgcagcgcc gggtgctgca cctgctgctc tatgccgtct gcgcgatcac ggcggggctg
    27421 ctcgccgtcg gcagggactt cttcctgctg atttcggggg cggagaaggc ctcctcggcg
    27481 ccgatttttg tctggatcgc cgtcaattac accctcttca cggtgctgat cgtctggacc
    27541 catggcctga cgttgctcaa gcggaccaat cggatcctgc tgaccgtcgg gggcgccgca
    27601 ctccttaacg tcgccctcaa catctggctt atcccgcgct atgtcgaaat gggggcggtc
    27661 tattcaaccc tgatcgcata cggggtgatg ggcctggcgc aggtcgcgct ctgcccgcgc
    27721 gactatctgc cgcggctgct gatcggcgac tggttgcggc cgctcgcgct ggccggcctg
    27781 atgctggccg tcgcctgggg aaccgatctg ttcgggctga cggcgtccat tccgcgactg
    27841 gccgccatgg cgggcgtctt cgtgatcgtg tttgtgatcc cggcctggtt catcgatcgc
    27901 gaaatgcggg agtatctgcg cgagaagctg gggatttgaa cagcgagcaa aggggcatcg
    27961 gagaatccgg gacgccggcc gggcctcgat tccgttacgt tccttaagtc ccttcagtct
    28021 ctccggcgcc tcggggtaga atagtgggcg gtccgccgcc gcgcggacga actcagtagg
    28081 cgaggagcag aggcgttcat ggccagaggc acgacatcgg caaagccgga gcagcccgac
    28141 aagggagcgc agggattcaa ggcgctcgat cgcaagctgg aattccaggg tctggaggcg
    28201 atcgataccg cggcgctgga ggctttcgat taccagtatc cgggcaaacc gatcacgatc
    28261 acgacgacga cggaggaatt cacttcggtc tgcccgttct ccgggctgcc cgatttcggc
    28321 cggatcacga tcgcctacgt gcccgatcgg cgttgcgtcg agttgcgctc gctcaagtac
    28381 tacctgatgt cgtaccgcaa cgtggggatt ttctacgagc atctggccaa ccggattcgc
    28441 gacgacctga tggcgctgct ggcgccgcgc agcctgcgcg tcgaatgccg gatgacgatc
    28501 cgcgggggtc tgcagacgac gatcatcgcc gaacacgagc aggcggcgcg atgagcggcg
    28561 cgggccagac gatcggcctg ttcggcggca gcttcaatcc gccgcatgtg gcgcatgttc
    28621 tggtcgtcgg ctgggcgctg gccggcggcg cggtcgacga ggtgtgggtg attccgacgg
    28681 gcgggcatcc gttcggcaag ccgctggcgc cgttcgagga tcggctggaa ctgtgccggc
    28741 gggcgttcgc ctgcttcggc ccgaaggtgc gggttctgga tctcgagcgc ggtccggacg
    28801 cgcagtactc gattgatacg ctgcggcggc ttcaggcgcg ccacccggac ctgcgctggc
    28861 ggtggatcat gggcagcgat caactggccg atgccgcgcg ctggaagaac ttcgacgaga
    28921 tcatgcggct ggcgccgccg ctggtcgtgc cgcgcgcggg ccacgcggtc cccggagcgt
    28981 cgggcgatgg cgcgccgccg cgtttcgccc tgcccgacct ttcctccacc ttcctgcgcg
    29041 agcggctcgc ggccggggct ttcgccgaac tggccggcct gatccccggc cccgtgctcg
    29101 atgagattga caaacggggc ctttaccggc cggtttagcg ttgaggggtg aaaccgcgcg
    29161 ccggatttgt tagcatggtg cttgagcttc cggtttccgc cgaaagcttg cggaagacca
    29221 ggccgaccgg gacggaatac gttcgatgcg gatcacgatg tgcaaggcca agctccaccg
    29281 ggccacggtg accgaggcgc aactgcatta cgagggatca atcacgatcg acgaggatct
    29341 gctcgaagct tccggcatcc tcgaatatga aaaggtgcag gtcgtcaata tcaacagcgg
    29401 ggcgcggttc gaaacctaca cctatgccgg tccgcgcggg tccggcatgg tctgcctcaa
    29461 tggcgcggcg gcgcgcctcg tgcaggtcgg cgaccggatt atcgtcattg cctactgcga
    29521 gctggaaccg gacgaggccc gcgaacacaa gccgacgctc gtgctgctgg aggacgataa
    29581 ctcaatcaag gaagtcgtcc gcaacggtcg gcacccggtg
//
LOCUS       DAOPCO010000019        28889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1770_length_28889_cov_5.938302, whole genome shotgun sequence.
ACCESSION   DAOPCO010000019 DAOPCO010000000
VERSION     DAOPCO010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28889
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1770_length_28889_cov_5.938302"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="PLS90_02840"
     CDS             <1..1804
                     /locus_tag="PLS90_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01372.1"
                     /translation="SLLLMALSEDWIDDESRELLAGLATTLEMGMTNCFHYANSLETE
                     QRYRDLWENAPAFFISILRGGIIFEINRTAAAALGYEQTALVGQPMRRLVHPDDHELF
                     DRMHRLLLETGQPQGYELRLVRAGGQPMVVSVNSEPLLDHRGGTIGEKSVLADITRDR
                     EMSERLRDYAENLERMVQERTVELTQTTNFLNGILEGSTEYAIVGLDGDGTFLTFNHG
                     AQLLFGYDAAEMVGAQKLELLINLEHSPWASSAELFRAVDRENVLVHETPMRTAHGRH
                     VIGLLTINRLKAPAANNLTYVAIIRDITEQKELEDLLKLYTENLQQLIEEKTRELDQK
                     HIQLIQSSKLATLGEMATGIAHELNQPLSGIRTRAQFILKAIERDQATIERIIAAQQE
                     IVQLVDRMSLIIHHMRIFARQDQQNFVPFRLVQSVDGALSLLGEQLRIHAIHVERQIE
                     DNLPMVYGEPLQIEQVILNLLSNARDSMDERAEALRGQQNGERPYQKRLKLTLRRVGD
                     KELCLEVRDNGVGMNDETQRKIFEPFFTTKPVGRGTGLGLSISYGILTNHKGRMDFKS
                     REGEGTTFRVYLPIWDGRSARRAVEESVEGELII"
     gene            2089..>2462
                     /gene="acpS"
                     /locus_tag="PLS90_02845"
     CDS             2089..>2462
                     /gene="acpS"
                     /locus_tag="PLS90_02845"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPK01373.1"
                     /translation="MAILGTGFDQTELSRIRDAIARFGDRFLNRCYTAEELRFAFLQK
                     DPVPRLAARYAAKEAGSKALGTGIARGILWREIEVTRRPGERPLIHFHGRAAQRAARM
                     GIRRAHVSLTHSRELGAAMVIV"
     assembly_gap    2463..2562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2563..3269)
                     /locus_tag="PLS90_02850"
     CDS             complement(<2563..3269)
                     /locus_tag="PLS90_02850"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK01374.1"
                     /translation="MDPIVEISTCSFDCIYCQLGRIQRVTAERREFIPAAWVREDLRG
                     VDWAAVDVVTISGSGEPTLATNLAAIIGEIRAATTKPILILSNATQFSDPEVRRGVAG
                     VDMIACKLDAPDDAMLARINRPAPGISLEAIVRGIVELRREFAGKLLLQVMFLPANIA
                     EVEAWAPLIRRIGPDEVHLNTPRRPYPLGWYRASRGDHDSAACPFEKRQLKVVTPAEA
                     CWAEARLRELTDVPFVSV"
     gene            complement(3232..3897)
                     /locus_tag="PLS90_02855"
     CDS             complement(3232..3897)
                     /locus_tag="PLS90_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK01375.1"
                     /translation="MRVRLFLSLAFLALALNAGAIPVYVGLFQVSDGPSWKENPRVLS
                     AREAAALIFGGHYTDYAISTERDTINHNAWLDGWGDTRYLAKPHHEDFKQGDYYLDAL
                     KQGYSFSAYVSDHTEAAAGRYNYVWRINGSGGGPVVPEPMTILMLGSGSLGIVMLRRR
                     RPASAARREFAPQPDDRPQHGHAPDGRVRRSTLDRLRPGRVVAFRPVARHRSDRRNLH
                     LFV"
     gene            complement(4147..5067)
                     /locus_tag="PLS90_02860"
     CDS             complement(4147..5067)
                     /locus_tag="PLS90_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019494609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01376.1"
                     /translation="MLGLQWGLAGAGLCVAWGWARPEARRAHAAGAFALAAYLLSLGM
                     LGAAGPAMGPGYHATMLVLAFTLALLWVGYAWLARHPPRSPPFVVRSQAEEVVWVNGA
                     TARGRRPAAHVLGAMVATAVHAVAIVLAARHLSLWFAAKDPLRAIVPQLLWLSLAAAL
                     LSASVLQALAFYGLGLIGWRADPDEPAGEHDLRQAARVTTTAAWILPATLLRMIAIGL
                     SLGVGAAFDPVSPRHFWDTVLTTSYQFLTLRLVLGLLLPLVMAILALLAAREGHARHA
                     VQQFLPLSILIVISEVLAAGLTVGLWGIAL"
     gene            complement(5422..5862)
                     /locus_tag="PLS90_02865"
     CDS             complement(5422..5862)
                     /locus_tag="PLS90_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK01377.1"
                     /translation="MFSQTSEYAIRALSILARYPRDQFILVGTLAQSAELPHHYLSKI
                     LQNLVRMQILESRKGSKGGFRLARDPNEITLYEIVNAIENLGQTRRCLLGQADCSDER
                     ACPLHEFWVEQTASYLDTLQHTSLGDLVRFDGKLRSTARPKLTV"
     gene            6403..7710
                     /locus_tag="PLS90_02870"
     CDS             6403..7710
                     /locus_tag="PLS90_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPK01378.1"
                     /translation="MSPQLETQGLKIETLCLHAGQQPDPSTNARGVPVHRTSSYVFRD
                     TEHAANLFGLKELGNIYTRLMNPTTDVLEKRMAALEGGVGGLGIASGTSAIFYSIINV
                     CRAGDEVVSAANLYGGTYTMFDQILPQFGIKVRLVDITDPQAIEGAINDNTRMIFCET
                     IGNPGLDVADLEMISGIARKHHLPLAVDSTFTTPYLLRPIEFGADIVIHSLTKWLGGH
                     GTGIGGIVVDSGKFDWTDKKFNLFNEPDESYHGLRYAHDLGDLTPLAFILRMRLVPLR
                     NLGACISPDNAWMFLQGIETLPLRMQRHSENAASVADYLKAHHLVDWVRYPGLEDDPC
                     HDLARKYLPNGCGGMVVFGIQGGRDAGARFINALQLHSHLANVGDAKSLAIHPATTTH
                     SQLNEQQLVEAGVRPEMVRLSVGLENIDDILADLDQALSASQA"
     gene            7843..9042
                     /locus_tag="PLS90_02875"
     CDS             7843..9042
                     /locus_tag="PLS90_02875"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPK01379.1"
                     /translation="MRALPTVETQFFTFAKSPEDAFVLESGDRFGPITLAYETWGELN
                     ADRSNAILLFHALTGSQHAAGFNPAVQGCEDLWTEECQIGWWNDFIGPGCALDTDRYF
                     VICINYLGGCYGSTGPRSINPATGRPYGGAFPTVTVRDTVDAQMRLLDHLGIQCLLAA
                     VGGSLGGMMVLELATRYPERVRCVIPLAAGHHATTLQRLHNFEAIFAIEEDRNYNYGD
                     YYDGDPPRMGLMLARMIAHKTFVHVDVMEERARGEIVQAEDDLKGYRLRHQIESYMLH
                     QGKKFVQRFDANAYLRILYMWQNFDLRARFNGDAVEAFKPCRHQRYLVFSIDSDVCFW
                     PEEQEELCQALKMADVPYQHITVHSDKGHDSFLLEPRFYAPYLSYVLDETWREIQAGQ
                     AAGDRAG"
     gene            9081..10136
                     /gene="trpD"
                     /locus_tag="PLS90_02880"
     CDS             9081..10136
                     /gene="trpD"
                     /locus_tag="PLS90_02880"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPK01380.1"
                     /translation="MEAISDAIMRLCTNEPVDEDLANRVAAEILSGAATAAQIGAFLV
                     GLAIRGEQAEHLKGFVRAMRSLAKPVTVTSHANLIDTCGTGGDHFCTFNISTAAAIVA
                     AGAGARIAKHGNRAVTSKQGSGSADVLAQLGVNVSCSTETSLRCLEQCGLAFFFAPTY
                     HGAMRHAGPARRELGVRTILNLLGPMTNPAGVRRQVIGVGIAEMTEVFARVLIETKSE
                     HVMVVHGQDGMDEITLTAPTKITEATQQEMKTYLFHPEEHGMALCKLADLQITAENQA
                     EARAEVIQEILAGTEGPWRDITVLNAAAALIVAGVARDWPDGIARARAAIDTRAAEKV
                     LETLVRVSNEEPPEAAG"
     gene            10136..10969
                     /gene="trpC"
                     /locus_tag="PLS90_02885"
     CDS             10136..10969
                     /gene="trpC"
                     /locus_tag="PLS90_02885"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPK01381.1"
                     /translation="MGILDRIRESKLAEVAAARSRVPEAELRARAAELGPTRGFAAAI
                     RGARPADQPRRPGGTIQVVAEVKKASPSVGVIRPDFDPVAIARAYAAGGAAAISCLTD
                     APFFQGSLEYLRRIREAVALPLLRKEFMLDPYQVWEARAAGADAILLIAGFVDNSLLA
                     DIRAVARAAGLDVLVEIHDADELDAALALDPDVLGINNRNLRDERFDTDLRRTLALAA
                     RVPPTQSFISESGIRTAADVERLRAAAIDGILVGEHLMREPDPGAAIRAKLGLGAAAR
                     P"
     gene            10999..11697
                     /locus_tag="PLS90_02890"
     CDS             10999..11697
                     /locus_tag="PLS90_02890"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HPK01382.1"
                     /translation="MFAVPGIDYIMEMLPWALGVFVALVLLFIFWKIWRAVVSNRVRK
                     IQTAAGMQFSDIDRMRKTGGITEEEYKRIRQAMARQTMQAMEEQRRIEAERSIFERAR
                     MNPEAAREFLRTGSAAPAADSGAAPGEAPTTPPNVAATPDPTPPRKPLPARPATAPAA
                     QPPPRRAPTPSMNMPRVVQERRRAQAQVPPESTANAEDRRAADAKAASELDVLLAKGA
                     ISKAEYDRLKQFFK"
     gene            12074..12379
                     /locus_tag="PLS90_02895"
     CDS             12074..12379
                     /locus_tag="PLS90_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HPK01383.1"
                     /translation="MDVRMDNQRVITSNIANLNTPGYAARKLNFEASLERALQDPAAA
                     DPVIEASAAPPQGLDGNNVVLEDELSALGRNKLLYTVTAQLYAAKLRQLASALDDQQ"
     gene            12403..12849
                     /gene="flgC"
                     /locus_tag="PLS90_02900"
     CDS             12403..12849
                     /gene="flgC"
                     /locus_tag="PLS90_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPK01384.1"
                     /translation="MSDFLGLDISGAGMSVQRQRLRVIAENLANQQTTGPDGPYQRKR
                     VVIEAAPVDSFAKAFDGALAERSDSDGATSVRVAAVRPDGAQPVRVYDPHHPHADRDG
                     YVELPNISVFREMAEMMEASRAYEANLAASKSTQDMLHSALDLLRR"
     gene            13173..13538
                     /gene="fliE"
                     /locus_tag="PLS90_02905"
     CDS             13173..13538
                     /gene="fliE"
                     /locus_tag="PLS90_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPK01385.1"
                     /translation="MDPISLQTHLRSLSGAGGLNELGSRAIEPAGRREIEPIGGLRAG
                     EDVSGPSFKDELTRSLEAVNNQMLEADRKTEALVSGQSGDLHGTIIAMQKADLSFRML
                     VEVRNKAIRAYEEVMRMQA"
     gene            13575..15398
                     /gene="fliF"
                     /locus_tag="PLS90_02910"
     CDS             13575..15398
                     /gene="fliF"
                     /locus_tag="PLS90_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPK01386.1"
                     /translation="MSFLLNLMRQTGTVLNQQSLARKVLLLAIIVGSVLAMVWLATRA
                     RTGGYVVLYSGLDPAETGRATARLVQSGIEAQPDNGGTTLKVERNRVDEAAILLAQEE
                     IPSTGGAGYELLGESPIGQSKFQQEKNYLRMRENELARTLQSIDGIERARVHLAIPDS
                     TVFLKETEVPTASVTVRLRRGTVLNDRQIRGITFLVSRSVEGMSPENVSLIDTEGNML
                     NAETDPASGDFSATYANFRAAKERELKESIESMLEETLGRGRVVARVQAEYDFATLHQ
                     VKESYNPDEQDPIITRESATREFQTSRNATVQVGEGAEMAEAEAAPAPGANAGGILRE
                     NTQTEYSVSKVVQESESRSPKLQRLSVAVLVDGEYEELTGEDGQVKREFRERSPEEMK
                     KYEELVKSAIGFTQNEERNDVISIQCAPFAFDTVEQLDEPFLNYEMQRLLEQVIQWGV
                     VGLLGLLIVLFVLRPALKQVLVTPIQVGPHALPAGAAGLGALTAGGESDGAVPGGMDA
                     NELERLAERLRSDKSLSEEVRQEMLAALRNDPETAKAARRLMDSGRGDSGHLAELQKL
                     ASRYVRESTAESHVIHREVMEVASQNPQKAVSLIRQWMDEA"
     gene            15524..16561
                     /gene="fliG"
                     /locus_tag="PLS90_02915"
     CDS             15524..16561
                     /gene="fliG"
                     /locus_tag="PLS90_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPK01387.1"
                     /translation="MALKGKPAQGNTHLSGMDKASIMLLSFDEASAAEVLRLMEEREI
                     QRLTSHASRLRHLSMEQINTVRQEFLMRLSDSSDLIISEARNKLKEILKKILPPDRYN
                     KFVEFLESGDELSEGFEALKWVDSQTIATYLRNEHPQTIAVILAHMETEKASEILLQV
                     PREIQADVIMRIATLDRINPELVRDIQDVIITEIMASGANKSRLVGGAQAVAEILNNM
                     DSQSEDAIFSTMEERNPDMADSIRELMFVFEDLLKIDDRGMKLIMKEVTNDMLTMALK
                     TAPEELREKIFKNISSRAAEMIREDLQTMGPTKLSDVERAQQEIVKVCRRLEAEGKIV
                     IASKGGGEIFV"
     gene            16554..17261
                     /locus_tag="PLS90_02920"
     CDS             16554..17261
                     /locus_tag="PLS90_02920"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HPK01388.1"
                     /translation="MSRIWRSQDLATRARSVDFSDQRLAPPARDLPRGAFQGLAAGER
                     AEPPLSHLERSELEARRLVESARAEAETIQRDAYHAGFEQGERAGRELALQKIEPTIQ
                     MFMNLIEGLKREREEQIRRHENELIKIAFLIAAHILRKQVELHPEVVREVVEAALAKV
                     DRTQHVHLFLAPNDCQLIQQQLAFFESHGWPPEHLHLEPDPTITRGGCRVETETGNID
                     ATIETQLKAMKTLLWDD"
     gene            17392..18765
                     /locus_tag="PLS90_02925"
     CDS             17392..18765
                     /locus_tag="PLS90_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPK01389.1"
                     /translation="MIAGADPIRTEGRVVQVIGTVIEGTLPDCAVGQLCEILPGGNEA
                     PLRAEVVGFRRDHVLIMPLGEMRGLKPGSRILSLRHAPTVGVGPDLLGRVIDGLGRPM
                     DGLPPVVPETHYPIYNDPPNAFQRERISEPLDLGVRAINGFLTCGVGQRVGIMAGSGV
                     GKSTLLGMIARQTRADINVIALTGERGRELRDFIERDLGEAGLARSVVVTATSDRSPL
                     ERVRAAFVATAIAEFFRDRGQSVLLMMDSVTRFAMAQREIGLSIGEPPTTKGYTPSVF
                     ALLPKLLERAGMGANGHGSITGLYTVLVDGDDLTEPIADATRAILDGHIVLSRQLADQ
                     GHFPAIDILASKSRLMTEIVTPDHLQSAQQMLGLLAAYQRAETLINIGAYVAGSNPTI
                     DRAIRMHEPLERFLRQGIFEAAAMEQCIHGLHQLVREASAPAPGRDNGRPGGQRVAEF
                     TPGGIGR"
     gene            18779..19261
                     /gene="fliJ"
                     /locus_tag="PLS90_02930"
     CDS             18779..19261
                     /gene="fliJ"
                     /locus_tag="PLS90_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPK01390.1"
                     /translation="MGFRFSLQPVLNHRKLLEERAQRDFSLARQRVEELRNMKQGLEE
                     DVANRGAQIRRAMLAGIGFAERSLWENWIQAQRAEALRLAREIDEAQRLADEKRARLV
                     HAMQQRTVLDRLRERELADWRREQDRAERRDFDEIAVRDYAEQMRQAKAAAQAERIAG
                     "
     gene            19270..19923
                     /locus_tag="PLS90_02935"
     CDS             19270..19923
                     /locus_tag="PLS90_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01391.1"
                     /translation="MNARTILRRKIFATLGCLLTLGFSLLLALRPAAGLPGTLFANPV
                     WAQDDPAPLIDPAREGLLAGEDATTTATQGWGTAGDAPFPLFASDPLHTLADSLRARE
                     RELERREREVAEAEQRLETVRLEIEQNLRRTEEVLAEMRRVAGEADQRREAELKKWIQ
                     IYQGMKPDKAGPMIGALEPQFQLDLLSRMDTRKAGKILESMDPQKAKSLALELAARQ"
     gene            19953..21365
                     /locus_tag="PLS90_02940"
     CDS             19953..21365
                     /locus_tag="PLS90_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPK01392.1"
                     /translation="MDLLSVPVAPPAGPAGAVPAGGSLLSAGDPASPFALLFGAALSL
                     GEFEQQLLAGSQLKAGGSPLARTAAGFAGETPFAAPAPVDAEEDAADEPEPDALNQSA
                     VGLFPPAGGDPLAGQRPVPVEGLPGEQAAGKSTWPARTAQDVSGLRPDTAGMKSAQST
                     SATVPAPVWNLRELAHHLRAALAVSADDARAAKQLAAGDVSAAPRPFQSAFDGAPLDA
                     RPAGQAGLASASPLEFPADDGGAPAVEASDQNAPAEAQAGDAAEPPPAAGKPLEQEPL
                     LDLDPAAHGDRMAELDSRATERVQPPTGAAAHEEWSPAPIDEAGQTRMPRLIEQVRQV
                     AGFLAERTDGVIRLGENGWQANLRLYPPDLGRVRVEMSVQPDRTVQAQLIAERPETAQ
                     LLDQSLQHLREALTRHGLTVERVTVVVQSAAATGGAESGMNQHASHDGRRQADLTQQR
                     QHNPQRHDGRGRQAFEEALG"
     gene            21386..22075
                     /locus_tag="PLS90_02945"
     CDS             21386..22075
                     /locus_tag="PLS90_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPK01393.1"
                     /translation="MNPITQVSALVSPPVSTGRSDSGNIVNKEEFLQLLVAQLQRQDP
                     LNPQDPAEFTSQLAQFSSLEQLINVNSSIASLANMQGMTAMVSAANFIGQNATVVGDA
                     LTVSEGKPSRVEFQMARDSVSTHMNVYDSSGALVAVVDLGARAAGENGFDWDGRLANG
                     ESLRDGNYRFEITAMDANNLLIDVLPMFEGRVDGISFDRGQVLLEINGRRIPMSELIR
                     VGAAAAQPSSN"
     gene            22114..23394
                     /locus_tag="PLS90_02950"
     CDS             22114..23394
                     /locus_tag="PLS90_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPK01394.1"
                     /translation="MSLLSALYTGVSGLQTFGDSLQVIGDNIANVNTIAFKASRAEFS
                     DLLSQSINGSAGRSQLGRGVSLERISANFAQGNFSNTDRLTDLAIEGNGFFIVNDGQR
                     NYYTRNGQLSLNAQGELTTNRGLNLMGFTFDSQGVLQRELVPLKIQNDSIPPRQTNGV
                     DVRLNLTSQVPATEIFTFDPANPVSTSNYSTSLTVYDSQGTAHSLTVYFNKVSNNNWE
                     WHAMEDGGEVGGTEGEPFEGAAGSLVFNENGALVSDETITNVGFNFQGTAQTIGFNFG
                     TSIAEGGNGLDGSTQYDQPSQVYTQTQDGFGTGSLRSITINDEGVIDGIYSNGQTIPI
                     GQITLANFANLQGLYKFGAGIYANTAESGQPIIDTPNVGGFGAIASYSLELSNVDLAT
                     EFVNLISTQRAYQANSKVITVGDQLMAEVVNIIR"
     gene            23443..24624
                     /locus_tag="PLS90_02955"
     CDS             23443..24624
                     /locus_tag="PLS90_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01395.1"
                     /translation="MHKPTIKGGLAGSGFAARFHFDALTRVHGVELQIAGIWSPTAPH
                     AEAFGRERGIPVLRDYDELLQAVDVVHLCTPPASHEPLALRALAAGRWVVIEKPLTGY
                     FGDGSESFSADGMDRRVALKEACASIERLLAAERRSRGRILYAENWVYAPAIQKEREI
                     LEKTGGQILWMLGGESHSGSHSPTYGLWRFSGGGSLMGKACHPLAGALYLKQVEGRAR
                     DGRPIRPATVSCRTHAITRMPAFRDEGHLRTGYHDVEDFAQAHVVFDDGTVADLFASE
                     LVLGGVHNFLEVAANNHRARININPNDALLTYNPVETNFSDIYVVEKIGTKQGWAPTS
                     PDEAWFTGYQHEMEAFYRAVARDEAPESGGPLGADCIATIYAAYVSAQHGGLETPVPR
                     L"
     gene            24793..25542
                     /locus_tag="PLS90_02960"
     CDS             24793..25542
                     /locus_tag="PLS90_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HPK01396.1"
                     /translation="MPAIAVFDAPFLQIPLAWIGLDRAVDISLLTLFGLIGNLLFSAR
                     VLVQWIASERSGRSVVPVSFWWISLVATLIHIVYAYARDDIPMILGSAVTLIPYVRNL
                     RIAHAPDHAPRSAGPLIVLSIVLSCAPAVFLFGREEYLGGLFWFGLLGNVVYRGRFFV
                     QWVQSETQRRSVMTLSFWYVSLLGSLMLLAYALLRGDFVFILSFLFNVVPYGRNVILI
                     YRHRRIEALDEKLKLAPERAPANSPERGGLG"
     gene            complement(25607..25813)
                     /locus_tag="PLS90_02965"
     CDS             complement(25607..25813)
                     /locus_tag="PLS90_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01397.1"
                     /translation="MNKTDPMPCCESLRGKSMYYRPDERPGRLHESDVMNYYCLHTQG
                     PVGPDGVEARPRLCQPGRACHVKS"
     gene            complement(25810..26781)
                     /locus_tag="PLS90_02970"
     CDS             complement(25810..26781)
                     /locus_tag="PLS90_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPK01398.1"
                     /translation="MQPFNYLIADTLDAALAEYAENRPVLKAGGIDLVDRMKERLATP
                     AVVLSLGEVEVLRYIEPEGEGVRLGCLTTLADIGRSELLNSRYAALAESAGHAATPQV
                     RERATLGGNLCQRPRCWYFRAIHFHCLKKGGATCFAVEGENQYHAVLGGGPCHIVHPS
                     NCAPPLVAMEALFVVRRAGAEERVIPAREFFVLPSSALDRENVLAPGEILTEVRIPRA
                     PDQSATIELREKQSFDWPLAMASVARVDGRRWNVCLGAVAPVPWVSEEAAEVLGSADI
                     TPELAARAGEAAAQAAQPMSDNGYKTQLIKVAVKRALLKAAGQEVPA"
     gene            complement(26781..>28889)
                     /locus_tag="PLS90_02975"
     CDS             complement(26781..>28889)
                     /locus_tag="PLS90_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HPK01399.1"
                     /translation="RGGRERLMTWRPDNRLALENDGSRVDVRDKVTGAARYTTDYYPE
                     GLLWASYIRCPYGKAFLRSSKLEAARAAPGVVEVELEKQEGTYPGDRLGHICAESRQA
                     LEQGLAALALEFERDRPATDIEREKKPLETFDEDPSVQEKQPDLERIFSEAAHTLERT
                     YQTQVQTHSCLEPHNSLVDYRGDHAIARQSTQATFSVRNELTEVLGLPPDKVEMHCEH
                     VGGGFGSKFGLSPEGQLAAKLSKKHGRPVRVLMNREEEHLDNGLRPGSLQYMKLAIGP
                     DGKLLGGRIHTWGSVGPVGGGGGTSNPSRYNFGIVAKTHQDVNLNSGFPRAMRAPGHP
                     QGMFAVEMMMDELAEAAGMDPLEFRRLNETSDVRRAMLDVGAEQIGWHRRKPSGTWEG
                     PLKRGFGIGVADWGNSPGEATITIHVYPNGNVEVLSGSQDIGTGFRTLLVDCVAHQLG
                     IDRARVEAKVGVSTYPPGPGSGGSVTSRFVAPKALGAAEQARQDLRQRVAREWGVPAD
                     EVAAENGVFKHADRTMSWNEACKLIGQDRLTVTEKENGIFWKEPTGSEAVQFAEVEVD
                     VETGIVRVKKVVALQNVGQAVNRKTVENQITGGVIQGISFALFEDRILNRQTGAMVNP
                     NLEFYKIAGSRDIPEIVPIIWQSRPDAGVNSLGEPPVVPTPGAVGCAVANAIGVPVRR
                     MPITPWRVLSALAERDGGAV"
BASE COUNT         5121 a         9798 c         9398 g         4472 t
ORIGIN      
        1 gtcgctgctg ctgatggccc tgagcgagga ttggattgat gacgagtcgc gcgagctgct
       61 cgcggggctc gccaccacgc tcgagatggg catgaccaac tgtttccact acgccaactc
      121 gctggagacc gaacagcgct accgcgacct gtgggaaaat gcgccggcgt tcttcatcag
      181 catcctgcgc ggcggcatca ttttcgaaat caaccggacc gccgccgccg ccttgggcta
      241 cgagcagacc gcgctggtcg gccagccgat gcgccggctc gtccatcccg acgatcacga
      301 gctgtttgac cgcatgcatc ggctgctgct cgaaaccggc cagcctcagg gctatgagct
      361 gcggctggtc cgcgccggcg gccagccgat ggtcgtcagc gtcaattccg aacccctgct
      421 cgaccatcgc ggcggcacga tcggcgaaaa aagcgtcctg gccgacatca ctcgcgaccg
      481 cgagatgagt gagcggctgc gcgactatgc cgaaaatctc gaacgcatgg tgcaggaacg
      541 caccgtcgaa ctgacccaga cgaccaattt cctcaacggg attctggagg gctcgaccga
      601 gtatgccatc gtcggcctcg acggcgacgg caccttcctg acgttcaacc acggcgcgca
      661 gcttctgttc ggctacgacg ccgccgaaat ggtcggcgcc cagaaactcg agctgctgat
      721 caacctggag cactcgccgt gggccagttc ggccgagctg ttccgcgccg tggaccggga
      781 gaacgtgctg gtccacgaga cgccgatgcg cacggcgcac ggccgccacg tgatcggcct
      841 gctgacgatc aaccggctca aggcgccggc ggcgaacaac ctgacctacg tcgcgatcat
      901 ccgcgacatc accgagcaga aggaactgga ggacctgctc aagctctaca cggaaaacct
      961 gcagcagttg atcgaggaga aaacccgcga actggaccag aaacacatcc agttgatcca
     1021 gtcgagcaag ctggcgacgc tgggcgagat ggcgaccggc atcgcgcacg agctgaatca
     1081 gccgctgtcg ggcatccgca cgcgcgcgca gttcatcctc aaggcgatcg agcgcgatca
     1141 ggcgacgatc gaacgcatca tcgcggcgca gcaggaaatc gtgcagctcg tcgaccggat
     1201 gtcgctcatc atccaccaca tgcggatttt cgcgcgccag gaccagcaga atttcgttcc
     1261 gttccgcctc gtccagtccg tcgatggcgc gctcagcctg ctcggcgagc agttgcggat
     1321 ccacgcgatc catgtcgagc gccagatcga ggacaacctg ccgatggttt acggcgagcc
     1381 gttgcaaatc gagcaggtga ttctgaatct gctgtccaac gcgcgcgact cgatggacga
     1441 gcgggccgaa gccctgcgcg gccagcagaa cggcgagcgc ccctaccaga aacgcctcaa
     1501 gctcacgctg cggcgggtcg gcgacaagga actctgcctc gaggtgcgcg acaacggcgt
     1561 cgggatgaac gacgaaaccc aacgcaagat cttcgaaccc ttcttcacga ccaagccggt
     1621 cggacgcggg acgggcctgg ggttgtcgat cagctacggg atcctgacca atcacaaggg
     1681 gcggatggat ttcaagtcgc gcgaggggga ggggacgacg ttccgagtct atctgccaat
     1741 ctgggatggc cgctcggccc gccgcgcggt cgaggaatcg gtcgagggcg aactgattat
     1801 ttagccgatc cccgcgtcga gggacgaaaa tacggagtga gcttctcgcg acgcctgcgg
     1861 cgtcaccctg aacggacgag aataggtggc cgtatacaac cgatccaaac ggcccaggcc
     1921 ttgccggacc gatcagccgg ccagccccgt cagggacggc agacttcagc ccgccacttc
     1981 agtggcggga tccgggatac caggagcaac agagtcccgc aggggcggca gaataattcg
     2041 gtccgaccac gaggacgagg gacgaagatt atgccgcgga gacacacgat ggccatcctt
     2101 ggcaccggct ttgaccagac cgaactgagc cgcatccgtg acgcaatcgc gcggttcggc
     2161 gaccggttcc tcaaccgctg ctatacggcg gaggaactcc gcttcgcctt cctgcaaaag
     2221 gatccggtcc cgcgcctcgc tgcgcgctat gccgccaagg aagccggctc caaggcgctc
     2281 ggcaccggga tcgcccgcgg catcctctgg cgcgaaatcg aggtgacgcg ccgacccggc
     2341 gagcgcccgc tgatccactt ccacggccgc gccgcccagc gcgccgcgcg catgggcatc
     2401 cgccgcgcgc acgtctcgct gacgcacagc cgcgaactgg gcgcggcgat ggtcatcgtg
     2461 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccgagac aaacggcacg
     2581 tcggtcaact cgcgcagccg cgcctccgcc cagcacgctt cggccggtgt caccactttt
     2641 aactgacgct tctcgaacgg gcaggccgcc gagtcgtgat cgccgcggct ggcccgatac
     2701 cagcccagcg gatacggccg gcgcggcgtg ttcaggtgca cttcgtccgg cccgatccgg
     2761 cggatcagcg gcgcccaggc ctccacttcc gcgatgttgg ccggcaggaa catgacctgc
     2821 agcaggagct tgccggcgaa ttcgcgccgc agctcgacaa ttccccgcac gatcgcttcc
     2881 agcgaaatcc ccggcgccgg ccggttgatc cgggccagca tcgcgtcgtc gggcgcatcc
     2941 agcttgcagg cgatcatgtc gacgccggcg accccccggc ggacctccgg atcgctgaac
     3001 tgcgtcgcgt tgctgagaat caggatcggt tttgtggtcg cggcgcggat ctcgccgatg
     3061 atcgccgcca gattggtcgc cagcgtcggc tcgccgctgc ccgagatcgt gacgacgtcg
     3121 accgccgccc agtcgacgcc gcgcagatcc tcgcgcaccc aggcggccgg aatgaactcc
     3181 ctccgctcgg cggtgacccg ctgaatgcgg ccaagctggc aatagatgca gtcaaacgaa
     3241 caggtggaga tttcgacgat cggatcgatg ccgagcgacc ggccgaaacg ccacgacgcg
     3301 accgggccgt aaacggtcga gggtcgagcg gcggacgcgt ccgtcggggg cgtggccgtg
     3361 ctggggtcgg tcatcaggct gcggggcgaa ctcccggcgt gccgcgctgg ctgggcggcg
     3421 acgcctcaac atcacgatac caaggctgcc gctccccagc atcaggattg tcattggctc
     3481 ggggacgacg ggaccgccgc cacttccgtt gatccgccag acgtaattgt agcgccccgc
     3541 ggccgcttcg gtgtggtcgc tcacatacgc cgaaaaacta tacccctgct tgagcgcatc
     3601 cagatagtag tcgccctgct tgaagtcctc gtggtgcggc ttggcaaggt agcgcgtgtc
     3661 gccccagcca tcgagccagg cgttgtggtt aatcgtgtcg cgttcggtcg agatggcgta
     3721 atcggtgtaa tggccgccga agatcagcgc cgccgcctcg cgggccgaca ggacgcgggg
     3781 gttctccttc cagctcgggc cgtcgctgac ttgaaagagc ccgacataga ccggaatggc
     3841 cccggcgttg agcgccagtg cgaggaaggc gagcgagaga aacagacgga cgcgcatcga
     3901 gactcctgga ctcctcaatc ggctgcacgg cgatatagcc ggagcaaggg ccggaccaga
     3961 tgccgggagc accagcggca ggaactcatc agatcggcaa gttcttcatt ctcagccatc
     4021 cgggcccgtc gaggcaaaac ggcgccgaag cacgcgtccg aaccagatcc tcgccacgcg
     4081 ccctcttgcc gccgcctcgt ctgcatcggg ttgcacatcg ctccgatgga cttcgtgtcc
     4141 ctttgatcac agcgcaatgc cccataaccc gacggtcaac ccggcggcga ggacctcgct
     4201 gatcacgata aggatcgaaa gcgggagaaa ctgctggacc gcgtggcgcg catgaccctc
     4261 gcgcgccgcc agcagcgcca aaatcgccat caccagcggc agcagcagcc ccaggaccag
     4321 ccgcagcgtc aggaactgat acgaggtggt caacaccgta tcccagaaat ggcgggggct
     4381 gaccggatcg aacgcggccc cgacgcccag gctcaggccg atggcgatca tgcgcagcag
     4441 ggtcgccggc aggatccagg cggcggtcgt cgtgaccctc gccgcctggc gcaggtcgtg
     4501 ctccccggcc ggctcatccg ggtcggcgcg ccagccgatc agccccaggc cataaaacgc
     4561 cagcgcctgc aggacgctgg cgctgagcag cgccgccgcc agcgagagcc acagcagttg
     4621 gggaacgatc gcgcgcaacg ggtccttggc ggcgaaccag agactcagat gccgcgcagc
     4681 caggacgatc gcgacggcgt ggaccgcggt ggcaaccatc gcgccaagca cgtgggccgc
     4741 cggccggcgc ccacgcgccg tcgcgccgtt gacccagacc acctcctccg cctgcgatcg
     4801 aacgacgaac ggaggcgacc gcggcgggtg ccgcgccagc caggcgtagc ccacccagag
     4861 cagcgccagc gtgaacgcca gcacgagcat cgtggcgtga tagcccggtc ccatcgccgg
     4921 gccggccgcc cccagcatgc ccagcgagag gaggtacgcg gccagggcga acgcccccgc
     4981 cgcgtgggct cggcgcgcct ccggccgggc ccagccccac gccacgcaca ggcccgcccc
     5041 cgccaggccc cactgcagcc ctaacatatg cacgaggatg aacaatcagg cggctcccgg
     5101 gatggcggag tcagtcggcg cgaggtccgt cagcgttgta gcacaagcgg cggcgatttt
     5161 gaatcgggcc ggcacggctg gcaagccgcg gggagcgaag gagggacggc caggggatca
     5221 ctcccgatca ttaggctccc tcatgacctt tatgtcccta gtatccctta cgtcccttac
     5281 gtcccttccg ctcccgagaa acagccgtcc acgaggacga gggacgagag acgcatgtgg
     5341 cgctgggaga aaaaaatcag ccggacgcgg gagcccgcgg gctcgcgctc cggccggttt
     5401 cgcttgcttc aagtcctggg gctagacggt cagcttgggc cgcgccgtcg agcgcagctt
     5461 gccgtcaaac cgcaccagat cgccgagcga cgtgtgctgc agcgtgtcga gatagctggc
     5521 ggtctgctcg acccagaatt catggagcgg gcaggcgcgc tcatcgctgc aatcggcctg
     5581 gccgagcagg cagcggcgcg tttgacccag gttctcgatc gcgttgacga tctcgtagag
     5641 cgtgatttcg ttgggatcgc gggccaggcg gaacccgccc ttgctcccct tgcgcgattc
     5701 gagaatctgc atccggacca gattttgcag aatcttgctc agatagtggt gcggcagttc
     5761 ggcgctttgc gccagcgtgc cgacaaggat gaactgatca cgcggatagc gcgccaggat
     5821 cgacaaggcg cgaatcgcgt attcgctggt ttgagagaac attgcctcgt tacctcatgt
     5881 gaaatgtctg ccgtggcagc cagggagagc gggagccagg ggacgcaggt gatgagtggg
     5941 gcgctcactc aaagcgggtt ggcggctccg gcgccccgat ccggaaccga gtggcggcag
     6001 atggaaagaa agccccggaa cagcgggcat cccagtgcat tcctgagtct taatatccta
     6061 atatcgagtc ctattgcaag tcattttgta tttacgatcc atacatctga ataatttcag
     6121 ctattcgact tgcgccggcg tcaacccatt ttccaattga atccgaccct cacccgcgat
     6181 acaagagagg ggcggctgcg atccccccga cggcgctcgc ggttcgcccc gctcgaccga
     6241 tcccttccga ctgctgacgg ccgactggtc ggctcttccc gcgggaagtg ggcgctggcc
     6301 ctgctcgcgc cagggggatt cggttatccc ggacttttgc ccgcgcccgc cgcggcaact
     6361 tgcgtgcttc acgcgatcga tctgcaacga ggaggccagt ccatgtcgcc ccagctcgaa
     6421 acgcagggcc tgaaaatcga aacgctctgc ctgcatgccg gccagcagcc cgaccccagc
     6481 accaacgcgc gcggcgtccc cgtccaccgc accagttcct acgtcttccg cgacaccgaa
     6541 cacgccgcca acctgttcgg cctgaaggaa ctgggcaaca tctacacgcg cctgatgaac
     6601 ccgacgaccg acgtgctgga gaagcgcatg gcggcgctgg aagggggcgt cggcggcctc
     6661 ggcatcgcct cggggaccag cgccatcttc tactcgatca tcaacgtctg ccgcgccggc
     6721 gacgaggtcg tctcggcggc caacctgtac ggcggcacct acacgatgtt cgaccagatc
     6781 ctgccccagt tcggcatcaa ggtccggctg gtcgacatca ccgacccgca ggcgatcgag
     6841 ggggcgatca acgataacac gcggatgatc ttctgcgaga cgatcggcaa ccccggcctc
     6901 gacgtggccg acctcgaaat gatctcgggg atcgcgcgca agcaccacct gccgctcgcc
     6961 gtcgactcca ccttcaccac gccgtatctg ctgcggccga ttgagttcgg cgccgacatc
     7021 gtcattcact cgctgaccaa gtggctcggc ggtcacggca ccggcatcgg cggcatcgtc
     7081 gtcgattcgg gcaagttcga ctggaccgac aagaagttca acctgttcaa cgaacccgac
     7141 gagagctacc acgggctgcg ctatgcgcac gatctgggcg acctgacccc cctggcgttc
     7201 atcctgcgca tgcggctggt gccgctgcgc aatctgggcg cctgcatctc gccggacaat
     7261 gcctggatgt tcctgcaggg gatcgagacc ctgccgctgc ggatgcagcg ccactcggag
     7321 aacgcggcgt ccgtggccga ttacctcaag gcgcatcacc tggtggactg ggtgcgctac
     7381 cccgggctgg aggacgaccc gtgccacgac ctggcgcgca agtacctgcc caacggctgc
     7441 ggcgggatgg tcgtcttcgg catccagggg ggccgcgacg ccggggcgcg gttcatcaac
     7501 gcgctgcaac tgcattcgca cctggccaat gtcggcgacg ccaagagcct ggcgatccac
     7561 cccgcgacga cgacccactc gcagctcaac gagcagcaac tggtcgaggc cggcgtgcgg
     7621 cccgaaatgg tccggctgtc ggtcggactg gagaacatcg acgatatcct ggccgacctg
     7681 gaccaggcct tgtcggcgtc gcaggcatga ccgccgcgac gcccatcgct cacggggggg
     7741 ccggcgtcgg catacgtcgg cccctgtctt ttcatcccac cctagccagt ctcaaggggc
     7801 ggacggccga tgcccggccg agggcctttg cggtaaggaa ccatgcgcgc tttgcctacc
     7861 gtcgagacgc aattcttcac gtttgccaag tctcctgaag atgcgtttgt tctcgaatcc
     7921 ggcgaccggt tcggtcccat cacgctcgct tacgaaacct ggggcgagct caatgcggat
     7981 cgctccaacg ccattctgct gttccatgcg ctgaccggca gtcagcacgc ggccggcttc
     8041 aaccccgccg tccagggctg cgaggatctg tggaccgagg agtgccagat cggctggtgg
     8101 aatgacttca tcgggccggg atgcgccctc gataccgacc gctacttcgt catctgcatc
     8161 aattatctgg ggggctgcta cggatcgacc ggcccccgct cgatcaaccc cgccaccggc
     8221 aggccgtacg gcggcgcgtt tcccaccgtg acggtccgcg acaccgtcga cgcccagatg
     8281 cgcctgctcg atcacctggg gatccagtgc ctgctggccg cggtcggcgg ctcgctgggc
     8341 ggcatgatgg tgctggagct ggcgacgcgc tatcccgagc gggtccgctg cgtcatcccg
     8401 ctggccgccg ggcatcatgc cacgacgctg cagcggctgc acaatttcga ggcgatcttc
     8461 gccatcgagg aagaccgcaa ctacaactac ggcgactact acgacggcga cccgccgcgc
     8521 atggggctga tgctggcccg gatgatcgcg cacaagacgt tcgtccacgt cgacgtgatg
     8581 gaggaacgcg cgcgcggcga gatcgtccag gccgaggacg acctgaaagg ctatcgcctg
     8641 cgccaccaga tcgaatccta catgctgcac cagggcaaga agttcgtgca gcgcttcgat
     8701 gccaacgcct atctgcggat cctctacatg tggcagaatt tcgacctgcg cgcgcggttc
     8761 aacggcgacg ccgtcgaggc gttcaagccg tgccgccacc agcgctatct ggtattctcg
     8821 atcgattcgg atgtctgctt ctggcctgag gagcaggagg aactgtgcca ggcgctcaaa
     8881 atggccgacg tcccgtacca gcacatcacc gtgcacagcg acaaggggca cgactcgttc
     8941 ctgctcgaac cgcggttcta cgccccctat ctctcctacg tgctggacga gacgtggcgc
     9001 gaaatccagg ccgggcaggc cgccggcgat cgtgcgggct gattcacgct cgcggcgata
     9061 cgccacaaga agggtcaccc atggaagcca tcagcgacgc tatcatgcgg ctctgcacga
     9121 acgagccggt cgatgaagac ctggccaacc gggtcgcggc cgagatcctg tcgggcgcgg
     9181 ccacggccgc ccagatcggc gcgttcctgg tcggcctggc catccgcggc gaacaggccg
     9241 agcatctgaa gggcttcgtg cgcgcgatgc gcagcctggc caagcccgtc accgtcacca
     9301 gccatgccaa cctgatcgat acctgcggga ccggaggcga tcacttctgc acgttcaaca
     9361 tctcgacggc ggcggcgatc gtcgcggccg gcgccggcgc ccgcatcgcc aagcacggca
     9421 atcgcgccgt caccagcaag caggggagcg gttcggccga cgtgctggcg cagctcggcg
     9481 tcaacgtcag ctgctcgacc gagacgagcc tgcgctgcct cgaacagtgc ggcctggcct
     9541 tcttcttcgc cccgacatac cacggcgcga tgcgccacgc cggcccggcc cgccgcgaac
     9601 tgggcgtccg cacgattctg aacctgctcg ggccgatgac caaccccgcc ggcgtgcgcc
     9661 gccaggtgat cggcgtcggc atcgccgaaa tgaccgaagt cttcgcccgc gtcctgatcg
     9721 aaaccaagtc cgaacatgtg atggtcgtcc atgggcagga tggcatggac gagatcacgc
     9781 tgaccgcccc gacgaagatc accgaggcga cccagcagga gatgaagacc tacctgttcc
     9841 atcccgagga gcacgggatg gcgctgtgca agctggcgga tctgcagatc acggccgaga
     9901 atcaggccga agcccgcgcc gaggtgatcc aggagatcct ggcgggaacc gagggtccgt
     9961 ggcgcgacat caccgtgctc aacgccgccg ccgcgctgat cgtcgccggc gtggcgcgcg
    10021 actggcccga cgggatcgcg cgcgcgcgcg ccgcgatcga cacccgcgcc gctgaaaagg
    10081 tcctcgagac cctcgtgcgc gtctcgaacg aggaaccgcc cgaggcggcc ggctgatggg
    10141 catcctggac cggatccgcg agagcaagct ggccgaggtg gcggcggccc ggtcgcgcgt
    10201 gcccgaggcc gagctgcgcg cgcgcgcggc tgagctgggg ccgacgcgag gcttcgccgc
    10261 ggcgatccgc ggcgcccgcc cggccgatca gccgcgtcgg cccggcggaa ccatccaggt
    10321 cgtcgcggag gtcaagaagg cgtcgccgag cgtcggcgtg atccgccccg atttcgaccc
    10381 ggtggccatc gcgcgcgcct acgcggcagg cggcgccgcc gcgatctcgt gcctgaccga
    10441 cgccccgttc ttccagggtt cgctggaata tcttcggcgc atccgcgaag ccgtcgcgct
    10501 tcccctcctg cgcaaggaat tcatgctcga cccgtaccag gtgtgggaag cgcgggcggc
    10561 tggggccgac gcgatcctgc tgatcgccgg tttcgtcgac aactccctgc tggcggatat
    10621 ccgcgcggtg gcgcgcgcgg ccggactcga cgtcctcgtc gaaatccacg acgccgacga
    10681 actggacgcg gcgctggcgc tcgaccccga tgtgctcggg atcaacaacc gcaacctgcg
    10741 cgacgagcga ttcgacaccg acttgcgccg gacgctggcg ctcgccgcgc gcgtgccccc
    10801 cacccaatcg ttcatctccg agagcggcat ccgcaccgcc gccgatgtcg agcggctgcg
    10861 cgccgcggcc atcgacggca tcctcgtggg cgagcatctg atgcgcgagc ccgaccccgg
    10921 cgcggctatc cgtgctaaac tcgggctggg ggcggcggcg cgcccctgat ccaccctccc
    10981 gctggccggc ggtaccccat gtttgccgtt cccggtatcg actacattat ggagatgctc
    11041 ccgtgggcgc tgggggtgtt cgttgcgctc gttctgctgt tcatcttctg gaagatctgg
    11101 cgcgccgtcg taagcaaccg cgtgcggaaa attcagaccg ccgccggcat gcagttctcg
    11161 gatatcgatc ggatgcgcaa gacgggcggc atcaccgagg aggaatacaa gaggatccgc
    11221 caggcgatgg cccgccagac gatgcaggcg atggaggagc agcggcggat cgaggccgag
    11281 cgctcgattt ttgagcgggc gcggatgaac ccggaggcgg cacgcgagtt cctgcggacc
    11341 ggctccgccg cgccggccgc cgacagcggc gccgcgccgg gggaggcgcc gacgacgccg
    11401 ccgaacgtgg ccgcgacccc cgaccctact ccgccgcgga aacccctgcc cgcccgaccg
    11461 gcaacggcgc cggcggcgca gccgcctccg cggcgggcgc ccacgccttc gatgaacatg
    11521 ccgcgcgtgg tgcaggagcg ccgccgcgca caggctcagg tcccgccgga gtcgactgcc
    11581 aacgcggagg atcggcgggc cgccgacgcc aaggccgcca gcgagctgga cgtgctgctg
    11641 gcaaagggcg cgatctccaa ggcggagtat gatcggttga agcagttttt taagtgaggc
    11701 cttcaggacc ggtgggggcg ggtgtgggac cggcgctccg ccgacctcag ccccccgcag
    11761 atcccccggc agaaagtgcc gtaacgttgg cgcgagggca gcaaatcgcc aattttatga
    11821 cgatacgccc tggatcgacc gctccccttg attggtttcg ccaaaatccc gacaatcccc
    11881 ttttgactca ataaaccatt acaaatcaat agtttataca aaattcgggc cgattctccg
    11941 cccggccggt gggcctctct ccgctccatc gccgtgaaaa aattgactgg cactcccctt
    12001 gcacatgcgg ggcgcccgga ggtgccttgt catggagata ttcggcgcga cctttaattt
    12061 gatggagcga gcgatggatg tccggatgga caaccagcgc gtgatcactt cgaatatcgc
    12121 caacctcaac acgccgggct acgcggcccg caagctgaat ttcgaggcct cgctggagcg
    12181 ggcgctccag gaccccgcgg cggcggaccc cgtcatcgaa gcctcggcgg ccccccctca
    12241 aggattggac ggcaacaacg tcgtcctgga agacgaactg tcggcgctgg gccgcaacaa
    12301 gctgctctat accgttacgg cccagcttta cgccgccaag ctgcgccagc tcgccagcgc
    12361 gctggatgat cagcagtaat tcgcgcggaa aggtgaacca acgtgagtga ttttctggga
    12421 ctcgacatca gcggcgccgg gatgagcgtc caacgccagc ggctgcgggt gatcgccgaa
    12481 aacctggcca accagcagac gacgggaccc gacggaccgt accagcgcaa gcgggtggtg
    12541 attgaagcgg cgccggtgga ctcgttcgcc aaggcattcg atggcgcgct ggcggagcgg
    12601 agcgacagtg atggcgccac gtcggttcgg gtcgccgcgg tgcgccccga cggagcgcaa
    12661 ccggtgcgcg tctatgatcc gcaccacccg cacgccgacc gcgacgggta tgtcgagctg
    12721 cccaacatca gcgtcttccg cgagatggcc gagatgatgg aggcctcgcg cgcttacgaa
    12781 gcaaacctcg ccgcgtcgaa gtcgacccag gacatgctcc acagcgcgct ggacctgctg
    12841 cggcgataac gaaggccgat cacgggttgc tcatgaaccg gcggttcaca acgcgggaat
    12901 gaaaatggag ggccctcaac caccgattcg aacgaccgcg gacgtgccgg ccccaacagc
    12961 cgcccagtcc tgtcagggac ggcagacttc agcccgccac ttcagtggcg ggaaaagatc
    13021 acagaaggaa cgaagagtcc cgtagggcga cagaaccgcc cgaatcgagc tgagttgctg
    13081 atgatccctc ggttcacaac gaggggccaa aatgggggcg ccaagagccg aggacgaggg
    13141 acgcatttca agcgtagggg aatggcttca gcatggatcc gatcagtctc cagacacacc
    13201 tgcggtcgct gagcggcgcc ggcggactca acgaactggg ctcgcgtgcg attgaaccgg
    13261 cgggccgccg ggagatcgag ccgatcggcg gcctgcgcgc cggcgaggac gtctccggcc
    13321 ccagtttcaa ggacgagctg acccggtcgc tcgaggcggt gaacaaccag atgctggagg
    13381 cggaccgcaa gaccgaggcg ctcgtcagcg gccagtcggg cgatctgcac gggacgatca
    13441 tcgcgatgca gaaagccgat ctctcgttcc gcatgctcgt ggaggtccgc aacaaggcga
    13501 tccgcgcgta tgaggaagtc atgcgcatgc aggcctgagc aagccgtcaa cccgtcgaag
    13561 tgagatagcg taagatgagc tttcttctca atctgatgcg ccagaccggc accgtcctca
    13621 atcagcagtc gctggcgcgc aaggtcctgc tgctggcgat catcgtcggc tcggtgctgg
    13681 cgatggtctg gctggcgacg cgggcgcgca cgggcggcta cgtcgtcctg tacagcggcc
    13741 tcgatccggc cgagacgggg cgggcgacgg cgcgcctggt gcagtcgggc atcgaggcgc
    13801 aacccgacaa cggcggcacg acgctcaagg tcgaacgcaa ccgcgtcgac gaagcggcga
    13861 tcctgctggc gcaggaagag atccccagca ccggcggcgc cggctatgaa ctgctgggcg
    13921 aatcgccgat cggccagtcg aagtttcaac aggaaaagaa ttacctgcgg atgcgcgaga
    13981 acgagttggc gcggacgctg cagtcgatcg acggcatcga gcgcgcgcgc gttcacctgg
    14041 cgatccccga cagcacggtc tttctgaagg agacggaggt gccgacggcg tcggtgacgg
    14101 tccggctgcg gcgcggcacg gtgctcaacg accggcagat ccgcggcatc acatttctcg
    14161 tctcgcgctc ggtcgagggg atgagtcccg agaacgtgtc gttgatcgac acggaaggga
    14221 acatgttgaa cgccgagacc gaccccgcct cgggcgactt ctccgcgacc tacgccaatt
    14281 tccgcgcggc caaggaacgg gaactgaagg agagcatcga gtcgatgctg gaggagacgc
    14341 tcggccgcgg ccgcgtcgtc gcgcgcgtcc aggccgaata cgacttcgcc acactgcacc
    14401 aggtgaagga atcctacaat cccgacgaac aggatccgat catcacccgc gagtcggcga
    14461 cgcgcgagtt tcaaaccagc cggaacgcga ccgtccaggt gggcgagggc gcggaaatgg
    14521 ccgaagcgga ggccgcgccg gcgccgggcg ccaacgcggg cggcatcctc cgcgagaaca
    14581 cgcagaccga atacagcgta tcgaaggtcg tgcaggagag cgaaagccgt tcccccaagc
    14641 tgcagcggct ctcggtggcc gtgctggtgg acggcgaata cgaggagctg accggcgagg
    14701 acggccaggt caagcgcgag ttccgcgagc gctcgccgga ggagatgaaa aagtatgagg
    14761 agctggtcaa gtcggcgatc ggcttcaccc agaatgagga acgcaacgac gtaatctcga
    14821 tccagtgcgc gccgttcgcc ttcgacacgg tcgagcaact ggacgaaccg ttcctgaatt
    14881 acgagatgca gcgcttgctc gagcaggtga ttcagtgggg ggtggtcggc ctgttgggtt
    14941 tgctgatcgt cctcttcgtg ctgcgtccgg cgctcaagca ggtgctggtg acgccgatcc
    15001 aggtcggtcc acatgcgctg ccggcggggg ccgccgggtt gggcgccctg acggccggcg
    15061 gggagagcga cggcgccgtg ccgggcggga tggatgcgaa tgaactggag cgcctggccg
    15121 aacggctgcg gtcggacaag tcgctctccg aagaagtccg gcaggagatg ctggcggcgc
    15181 tgcgcaatga tccggaaacg gcgaaggccg ccaggcggct gatggacagc ggccgcggcg
    15241 actcaggtca cctggccgaa ctgcagaagc tggcatcacg ctacgtgcgc gaatcgaccg
    15301 ccgaatccca cgtgatccac cgcgaggtga tggaggtggc cagccagaat ccgcagaagg
    15361 cggtcagtct gatccgccag tggatggatg aggcctgagc cgctgaggct cgggcagcgg
    15421 gagcgcgcgc ctcgggagtt gtcggcccga agcgagcacg gagccgggcg gcccgcaaac
    15481 gggcccccgc tggatgaatc gacccggatg gatgggacgc gagatggcgc ttaagggaaa
    15541 accagcccag ggcaacacgc atctgagcgg catggacaag gcctcgatca tgctgctgtc
    15601 gtttgacgaa gcttcggcgg ccgaagtgct gcggctgatg gaggagcgcg agatccagcg
    15661 cctgacgagc catgcctcgc ggctgcggca cctctcgatg gaacagatca acaccgtccg
    15721 ccaggagttt ttgatgcggc tctcggattc gagcgacctg atcatcagcg aggcgcgcaa
    15781 caagctgaag gagatcctca agaagatcct gccgcccgac cgctacaaca agttcgtgga
    15841 gttcctcgaa tcgggcgacg agctctcgga gggcttcgag gcgctcaagt gggtcgattc
    15901 ccaaaccatc gccacctacc tgcgcaacga gcacccgcag acgatcgccg tcatcctggc
    15961 gcacatggag accgagaagg cgagcgaaat cctgctgcag gtcccgcgcg agattcaggc
    16021 cgacgtgatc atgcggatcg cgacgctcga ccggatcaac ccggaactgg tgcgcgacat
    16081 ccaggacgtg atcatcaccg agatcatggc ctcgggcgcc aacaaaagcc ggctggtggg
    16141 tggcgcccag gccgtcgccg agatcctcaa caacatggac agccagagcg aggacgcgat
    16201 cttcagcacg atggaggagc gcaatcccga catggccgac tcgatccgcg agctgatgtt
    16261 cgtcttcgag gatctgctca agatcgacga ccgcggcatg aagctcatca tgaaggaagt
    16321 gaccaacgac atgctgacga tggcgctgaa gacggcgccc gaggagctgc gcgaaaagat
    16381 cttcaagaac atctcatcgc gcgcggccga gatgatccgc gaggacctgc agacgatggg
    16441 cccgaccaag ctgtccgacg tcgaacgggc ccagcaggaa atcgtcaagg tctgccgccg
    16501 cctcgaggcc gaggggaaaa tcgtgattgc cagcaagggc ggaggcgaga tctttgtcta
    16561 gaatctggcg ttcccaggac ctggcgacgc gcgcgcggtc ggtcgatttc agcgaccagc
    16621 gcctggcgcc gcccgcgcgc gatctgccgc gcggcgcatt ccaggggctg gccgccggcg
    16681 aacgcgcgga accgcccctc tcgcatctcg aacggagcga gctggaggcg cggcggctcg
    16741 tcgaatcggc gcgcgccgaa gccgaaacca ttcagcgcga cgcctaccac gcaggcttcg
    16801 agcagggcga gcgcgccggc cgcgagctcg cgctgcagaa gatcgaaccg acgatccaga
    16861 tgttcatgaa cctgatcgaa ggattgaagc gcgagcgcga ggaacagatc cgccggcacg
    16921 aaaacgaact gatcaagatc gcgttcctga tcgccgcgca tatcctgcgc aagcaggtcg
    16981 agttgcatcc ggaggtcgtg cgcgaagtcg tcgaggccgc gctggccaag gtcgatcgga
    17041 cgcagcatgt ccacttgttt ctggcgccca atgactgtca gctcatccag cagcagcttg
    17101 ccttctttga aagccatggc tggccccccg agcacctgca cctggaaccc gatccgacga
    17161 tcacacgcgg cggctgccgg gtcgagactg aaacgggcaa catcgacgca acgatcgaaa
    17221 cgcaactgaa ggcaatgaaa acgctgctgt gggacgactg aacggaacgc ccttccgcct
    17281 cgccggcgga gtcgaatcac cggatgcctc caacaagatg acgggaatgt gcgccgcggc
    17341 gcgagtggat gaaggatcga gcgagtgttc aaggcggaac aatatctacg gatgattgcc
    17401 ggggccgacc cgattcgcac cgaggggcgc gtggttcaag tcatcggcac cgtgattgaa
    17461 gggacgctgc ccgactgcgc ggtcggccag ctctgcgaaa tcctcccggg cggcaacgaa
    17521 gcccccctgc gcgccgaagt ggtcggcttc cgtcgcgacc acgtgttgat catgccgctg
    17581 ggcgaaatgc gcggcctcaa gccgggcagc cggatcctct cgctgcgcca cgcgccgacg
    17641 gtcggcgtcg gccccgacct gctcgggcgg gtgatcgacg gcctcggccg cccgatggac
    17701 ggcctgcccc ccgtggtgcc cgagacgcac tatccgatct ataacgaccc gcccaatgcc
    17761 ttccagcgcg aacggatttc ggagccgctc gatctgggcg tgcgggcgat caatgggttt
    17821 ctgacctgcg gcgtcggcca gcgcgtcggc atcatggccg gttcgggcgt gggcaagtcg
    17881 acgctgctgg ggatgatcgc gcgccagacc cgggccgaca tcaatgtcat cgcgctgacg
    17941 ggcgaacgcg gccgcgagct gcgcgacttc atcgagcgcg atctgggcga ggcggggctc
    18001 gcccgctcgg tcgtggtgac cgccacgtcg gatcgctcgc cgctggagcg cgtccgcgcg
    18061 gcgttcgttg ccacggcaat cgccgagttc ttccgcgacc gcggccagag cgtgctgctg
    18121 atgatggact cggtcacgcg atttgccatg gcgcagcgcg aaatcggcct cagcatcggc
    18181 gaaccgccga cgaccaaggg ctacaccccg tcggtcttcg cgctgctgcc caagttgctc
    18241 gagcgcgcgg gcatgggcgc caacggccat ggcagcatca ccgggcttta caccgtgctg
    18301 gtcgacggcg acgacctgac cgaaccgatc gccgacgcga cgcgcgcgat cctggacggc
    18361 cacatcgtgc tgtcgcgtca gcttgccgac caggggcatt tcccggcgat cgatattctg
    18421 gccagcaaga gccgtctgat gaccgagatc gtcacgcccg atcacttgca gtcggcgcag
    18481 cagatgctcg gcctgttggc ggcctatcaa cgcgccgaga cgctgatcaa catcggcgcc
    18541 tacgtggccg gctccaatcc gacgatcgac cgggcgatcc gcatgcacga gccgctggag
    18601 cgctttctgc gccaggggat cttcgaggcg gcggccatgg aacagtgcat ccatggcctg
    18661 catcaactgg tgcgcgaggc ctcggccccc gcgcccggac gcgataacgg gcggcctggc
    18721 ggtcagcgcg tcgccgagtt cactcccggc ggcattggcc gctgaaaggt agaacgcgat
    18781 gggctttcgc ttctcgctgc aaccggttct gaaccaccgc aagctgctgg aggaacgcgc
    18841 gcaacgcgac ttcagcctgg cgcgtcaacg cgtcgaggaa ctgcgcaaca tgaagcaggg
    18901 cctggaggag gatgtcgcca atcggggcgc gcagatccgc cgcgcgatgc tggccggcat
    18961 cggcttcgcc gagcgcagcc tgtgggagaa ctggatccag gcccagcgtg ccgaggccct
    19021 caggctggcg cgcgagatcg acgaggcgca gcgcctggcc gacgagaaac gcgcgcgcct
    19081 ggtccacgcg atgcagcagc gcacggtgct ggaccggctg cgcgaacgcg agctggccga
    19141 ctggcggcgc gagcaggatc gcgccgagcg cagggacttc gacgagatcg cggtgcgtga
    19201 ctatgccgaa cagatgcggc aggcaaaagc tgccgcgcag gcggaaagga ttgcagggtg
    19261 aatcggacca tgaatgccag gacaattctg cgacggaaaa tcttcgccac gctcggatgc
    19321 ctgctgacgc tgggtttcag cctgctgctg gcgctgcggc cggccgcggg ccttccgggc
    19381 acgctctttg ccaatccggt ctgggcgcag gatgaccccg cgccgctgat cgatccggcg
    19441 cgcgaaggac tgctcgccgg cgaggatgca acgacgaccg cgacgcaggg atggggaacc
    19501 gccggcgacg cgcccttccc gctcttcgcc tccgacccgc ttcacacgct cgccgactcg
    19561 ctgcgcgcgc gcgaacggga gctggaacgg cgcgagcggg aggtcgccga ggccgaacag
    19621 cgactcgaaa cggtgcggct ggagatcgag cagaaccttc gccggaccga ggaagtcctc
    19681 gccgaaatgc ggcgcgtcgc cggggaagcc gatcagcgcc gcgaagccga actgaagaaa
    19741 tggattcaga tttaccaggg catgaagccg gataaggccg gtccgatgat cggcgcgctg
    19801 gagccgcaat tccagttgga tctgctctcc cggatggata cgcgcaaggc cggcaagatc
    19861 ctcgaaagca tggatccgca gaaggccaaa tcgctggcgc ttgagctggc ggcgcgtcaa
    19921 taactgccgc aagactgctc ccgaggttgt tcatggatct gttgtccgtt cccgttgcac
    19981 cccctgccgg ccccgccggc gccgtcccgg cgggcggttc cctgctctcc gccggcgatc
    20041 ccgcctcacc gttcgcgctg ctgtttgggg cggcgctctc gctgggcgaa ttcgaacagc
    20101 aactgctggc gggttctcag ttgaaagccg gcggctctcc cctcgcccgg acggcggcgg
    20161 gatttgccgg tgaaacacct ttcgccgcgc ccgcgccggt tgacgccgag gaggatgccg
    20221 ccgacgaacc cgagccggac gccttgaacc agagtgcggt cggcttgttt ccgcccgcgg
    20281 gcggcgatcc ccttgcgggc cagcgcccgg ttccggtcga agggctgccg ggtgagcagg
    20341 ccgcgggtaa gtccacctgg cccgcccgga cggcccagga cgtctcaggc ctgcgcccgg
    20401 atacggcggg aatgaaatcg gctcagtcga ccagcgcgac ggttcccgct ccggtctgga
    20461 atctgcgtga gctggcgcat cacctgcgcg ccgcgctggc ggtgtcggcc gacgacgcgc
    20521 gggccgcgaa gcagttggcg gcgggcgatg tatcggccgc gccgcggccg ttccaaagtg
    20581 catttgacgg cgcgccgctg gacgcgcggc cagccggaca ggctggactc gcaagcgcca
    20641 gccctctcga attcccagcg gatgacggcg gcgcccccgc tgttgaagcc tccgaccaga
    20701 acgccccagc cgaggctcag gccggcgacg ccgcggagcc cccgccggcg gcgggaaagc
    20761 cgcttgagca agaaccgctg ttggatctgg acccggccgc gcacggcgac cgtatggctg
    20821 agttggactc gcgcgcaacg gagcgcgtcc aaccgccgac cggcgccgcc gcgcacgagg
    20881 aatggagtcc cgcccccatc gacgaggcgg gccagacccg catgccgcgc ctgattgaac
    20941 aggtgcgcca ggtcgccggc ttcctggccg agcggaccga tggcgtgatc cggctgggcg
    21001 agaacggatg gcaagcgaac ctgcgccttt atccgcccga cctgggccgc gtgcgggtcg
    21061 agatgagcgt gcagccggac cgcaccgtcc aggcgcaatt gatcgccgaa cgccccgaga
    21121 cggcgcaact gcttgatcag tcgctccagc acctgcgcga ggcgctgacg cgccacggcc
    21181 tgacggtcga acgcgtgacg gtcgtcgtgc agtcggcggc tgccaccggc ggcgccgagt
    21241 cgggcatgaa ccagcacgcg tcgcatgatg ggcgacggca ggccgacctg acccagcagc
    21301 gccagcacaa cccgcagcgg cacgacggcc gcggccggca ggcgtttgag gaagcactgg
    21361 gctgagcgcc cgacaggagt tgaccatgaa cccgatcacg caagtctccg cgctcgtctc
    21421 gccgccggtc tccaccggcc gcagcgacag cggcaacatc gtcaacaagg aggagttcct
    21481 ccagctcctg gtggcgcagc ttcagcgcca agacccgctc aacccgcagg atccggcgga
    21541 gttcacgtcg caactggcgc agttcagcag cctggagcag ctcatcaacg tcaacagcag
    21601 catcgcttcg ctggcgaaca tgcaggggat gacggcgatg gtgtcggcgg cgaacttcat
    21661 cggccagaac gccaccgtcg tcggcgacgc gttgacggtc agcgagggca agccgagccg
    21721 ggttgagttc cagatggcgc gcgacagcgt ctcgacgcac atgaacgtct acgactcaag
    21781 cggggcgctc gtggcggtcg tcgacctggg cgcgcgcgcg gccggcgaga acggcttcga
    21841 ttgggacggc cggctggcca acggcgagag cctgcgcgac ggcaactacc gcttcgaaat
    21901 cacggccatg gatgccaaca acctgctcat tgacgttttg ccgatgttcg aggggcgggt
    21961 cgacgggatc agcttcgacc gcggccaggt cctgctcgaa atcaacggcc ggcggattcc
    22021 gatgagcgag ctgattcgcg tcggcgccgc cgccgcgcaa ccttcttcga attagcaact
    22081 tcagcacaag taacagccca aaggagagct ctcatgtcac tgcttagtgc gctctacacc
    22141 ggtgtcagcg gtctgcagac gttcggtgac agcctgcagg tgatcggcga caatatcgcc
    22201 aacgttaaca cgatcgcctt caaggcgtcg cgcgcggaat tctcggacct gctcagtcag
    22261 tcgatcaacg gcagcgccgg ccgcagccaa ttgggtcgcg gtgtgtcgct cgagcgcatc
    22321 tcggccaatt tcgcgcaagg caacttctcc aacactgacc gcctgaccga cctcgccatc
    22381 gagggaaacg gcttcttcat cgtcaacgac ggccagcgca actactacac gcgcaacggc
    22441 cagctttcgc tcaacgctca aggcgaactg acgaccaacc gcggtctgaa cctgatgggc
    22501 ttcaccttcg attcccaggg cgtactccag cgcgagctgg tgccgttgaa gatccagaac
    22561 gacagcatcc cgccgcggca aaccaacggc gtcgacgtgc ggctgaacct gaccagccag
    22621 gtcccggcca ccgaaatctt cacgttcgat ccggccaatc cggtctccac gtcgaattac
    22681 tcgacgagcc tgaccgtcta cgactcgcag ggcacggctc acagcctgac cgtctacttc
    22741 aacaaggtct ccaacaacaa ctgggaatgg cacgcgatgg aggacggcgg cgaggtcggc
    22801 ggcacggagg gcgaaccgtt cgagggagcg gccggctcgc tcgtgttcaa tgaaaacggc
    22861 gcgctcgtct ccgacgagac gatcaccaac gtcggcttca acttccaggg gacggcgcag
    22921 accatcggct tcaacttcgg cacctcaatc gccgaaggcg gcaacgggct ggacggctcg
    22981 acccagtacg accagccgag ccaggtttac acgcagacgc aggacggatt cggcaccggc
    23041 tccctccgtt cgatcacgat caatgatgaa ggggtgatcg acggcattta cagcaacggc
    23101 cagacgattc cgatcgggca gatcacgctg gccaacttcg ccaacctgca ggggctgtat
    23161 aagttcggcg cggggatcta cgccaacacc gccgagtcgg gccagccgat catcgacacc
    23221 cccaatgtcg gcgggttcgg cgccatcgcc tcctacagcc tggagctttc caatgttgac
    23281 ctggccaccg agttcgttaa cctgatctca acgcagcgcg cctatcaggc gaactcgaag
    23341 gtcatcaccg tgggcgatca gctcatggcc gaagtggtca acatcatccg ttaaccaccg
    23401 gccgcgcggg ctgaacggcg gcccgcgccg ctggagcgga tcatgcacaa accgacgatc
    23461 aagggcggcc tggccggttc cggcttcgcc gcccgttttc attttgacgc acttacccgc
    23521 gtccacggcg tcgagcttca gattgccggg atctggtcgc ccacggcccc ccacgccgag
    23581 gccttcggcc gcgaacgcgg cattcccgtc ctgcgcgatt acgacgaact gctgcaggcg
    23641 gtggacgttg tgcatctgtg cacgccgccc gcgtcgcacg agccgctggc gctgcgggcg
    23701 ctggcggccg ggcgctgggt cgtcattgaa aagcccctga ccggctattt cggcgacggg
    23761 tccgagtcgt tttcggccga cggcatggat cggcgcgtcg cgctgaagga ggcctgcgcc
    23821 agcatcgagc gcctgctggc ggcggaacga cgcagccgcg ggcgcatcct gtacgccgag
    23881 aactgggtct acgcccccgc catccagaag gagcgggaaa ttctcgaaaa aaccggcgga
    23941 cagatcctct ggatgctcgg cggcgagtct cactccggct cgcattcgcc gacctacggc
    24001 ctctggcgct tctcgggcgg cggcagcctg atgggcaagg cctgccaccc gctcgccggc
    24061 gcgctctacc tgaaacaggt cgaagggcgc gcgcgcgacg gccggccgat ccgcccggcg
    24121 accgtctcgt gccgcaccca cgccatcacg cggatgcccg cgttccgcga tgagggccac
    24181 ctgcgcaccg gttaccacga cgtcgaggat ttcgcccagg cgcacgtcgt gttcgacgac
    24241 ggcacggtcg ccgacctctt cgcctccgag ctcgtcctgg ggggcgttca caatttcctc
    24301 gaggtggccg ccaacaacca ccgcgcgcgc atcaacatca accccaacga cgcgctgctg
    24361 acctacaacc ccgtcgagac aaatttcagc gacatctacg tcgtcgaaaa gatcggcacg
    24421 aagcaggggt gggcgccgac ctcgcccgac gaagcgtggt tcaccggcta tcagcacgaa
    24481 atggaagcgt tctaccgcgc ggtggcgcgc gacgaggcgc ccgaaagcgg cggcccgctc
    24541 ggcgccgatt gcatcgcgac gatctatgcg gcctacgtct ccgcccagca cggcggcctg
    24601 gaaacccccg tcccgcggct gtagcccgac gaagctctgt caggtccatc cgatccgacg
    24661 gattgcgagc gcccgcgccg gcatgcgccg cattgaaagc cgccgttaat cggtctactg
    24721 tgaagcgccg cgtgagggaa tcagaacccc gcgcggacga gcgccgcgcc cgcgccgcgc
    24781 cgactctgcc ccatgccggc catcgccgtg tttgatgctc cttttctcca aatcccgctt
    24841 gcgtggatcg ggctggaccg ggccgtcgat atctcgctgc tgacgctgtt cgggctgatc
    24901 ggcaacctgc tgttcagcgc gcgtgtcctc gtgcagtgga tcgccagcga acggtcgggc
    24961 cgcagcgtcg tccccgtctc gttctggtgg atcagtctgg tcgccacgct gatccacatc
    25021 gtctatgcct acgcgcgcga cgacatcccg atgatcctcg gctcggcggt cacgctgatc
    25081 ccatatgtcc ggaatctgcg gatcgcccac gcgccggacc acgcgccgcg atcggccggg
    25141 ccgctcattg tgctctcgat cgtgctgagc tgcgcccccg cggtcttcct cttcggccgc
    25201 gaggaatacc tcggcggcct gttctggttc ggcctgctgg gcaacgtggt ctatcgcggc
    25261 cggttcttcg tgcagtgggt gcagagcgag acccagcggc gcagcgtcat gaccctctcg
    25321 ttctggtacg tcagcctgct ggggtcgctg atgctgctgg cctatgccct gctccgcggc
    25381 gacttcgtct tcatcctcag cttcctgttc aatgtcgtcc cctacgggcg gaacgtcatt
    25441 ctgatctacc gccaccggcg aatcgaagcg ctggacgaga agctgaagct ggcgccggaa
    25501 cgcgctcccg ccaacagccc ggagcgcggc ggactgggct gagacgctgg ttttccctca
    25561 gaatgaaatt ccggctccgt cgtgccggga cttccgaagg gaggaatcag gatttcacat
    25621 ggcaggcccg gccgggctgg cacaggcgcg ggcgcgcctc caccccgtcg ggccccaccg
    25681 gcccctgcgt atgcaggcag tagtaattca tcacgtcgct ttcgtggagc cggcccggcc
    25741 gttcatccgg ccggtagtac atgctcttgc cgcgcaggct ttcgcagcac ggcatcgggt
    25801 cggtcttgtt catgccggaa cctcctggcc ggcggccttg agcagcgcgc gcttgaccgc
    25861 caccttgatc agctgcgtct tgtagccatt gtccgacatc ggttgggcgg cctgggccgc
    25921 cgcctcgccg gcgcgcgccg ccagttccgg cgtaatgtcg gcgctcccga gcacctcggc
    25981 cgcttcctcg ctgacccacg gcaccggggc caccgcgccc aggcagacgt tccaccggcg
    26041 gccgtcaacc cgggcaaccg aagccatcgc cagcggccag tcgaacgact gcttctcgcg
    26101 cagttcaatc gtcgcgctct ggtcgggcgc cctggggatc cgcacttcgg tcaggatttc
    26161 ccccggcgcc agcacattct cccgatccag cgccgaggag ggcaggacga agaactcgcg
    26221 cgccgggatc acgcgctcct ccgcgccggc gcgacgcacg acgaacaacg cctccatcgc
    26281 caccagcggc ggcgcgcagt tggagggatg cacgatgtgg cacggcccgc cgcccagcac
    26341 cgcgtgatac tgattctcac cctccacggc gaaacacgtc gcgcccccct tcttcaaaca
    26401 atggaagtgg atcgcgcgga aataccagca gcgcggccgc tggcacaggt tgccccccag
    26461 cgtggcgcgc tcgcgcacct gcggcgtggc ggcatggccg gccgattcgg ccagcgcggc
    26521 gtagcggctg ttgagcagct cgctgcggcc gatgtcggcc agcgtcgtca ggcaaccgag
    26581 ccgcaccccc tccccctccg gctcgatgta gcgcaatacc tccacctcgc ccagcgacag
    26641 cacgaccgcg ggggtcgcca gccgctcctt catccggtcg accagatcga tgccgccggc
    26701 cttgagcacc gggcggttct cggcatactc ggccagcgcg gcgtcgagcg tatcggcgat
    26761 caggtaattg aacggttgca tcagacggcg cctccgtcgc gttcggccag cgcgctcagc
    26821 acgcgccatg gcgtgatcgg catccgccgg accggcacgc cgatcgcgtt ggcgacggcg
    26881 cagccgaccg ctcccggcgt cggcaccacc ggcggctcgc ccaggctgtt cacccccgcg
    26941 tccgggcggc tttgccagat gatcggaacg atctccggaa tgtcgcgcga gccggcgatc
    27001 ttgtaaaact ccaggttcgg attgaccatc gcgccggtct ggcggttcag gatccgatcc
    27061 tcgaacaagg caaagctgat cccctggatc acgccgccgg tgatctggtt ttccactgtc
    27121 ttgcggttga ccgcctggcc gacgttctgc agcgccacca ccttcttcac gcggacgatc
    27181 cccgtctcga cgtccacctc cacttcggcg aactgaaccg cctcgctgcc ggtcggttct
    27241 ttccagaaaa tcccgttttc cttctccgtc acggtcagcc ggtcctggcc gatcagcttg
    27301 cacgcctcgt tccacgacat cgtgcggtcg gcgtgcttga agacgccgtt ttcggcggct
    27361 acctcgtcgg ccggcacgcc ccattcgcgc gccacgcgct ggcgcaggtc ctgcctggcc
    27421 tgctcggccg cgcccagtgc cttcggcgcc acgaaccggc tcgtcaccga accgccgctg
    27481 cccggccccg gcggataggt gctgacgccg accttcgcct ccacgcgcgc gcggtcgatg
    27541 ccgagctggt gcgccacgca gtcgaccagc aacgtgcgga acccggtgcc gatatcctgg
    27601 ctgccgctca gcacctcgac attgccgttg gggtaaacgt ggatcgtgat cgtggcctcg
    27661 cccgggctgt tgccccagtc ggccacgccg atgccgaagc cgcgtttgag cggcccctcc
    27721 cacgtgcccg acggcttgcg ccggtgccag ccgatctgct cggcgcccac atccagcatc
    27781 gcgcggcgga cgtcgctcgt ctcgttgagc cgccggaatt ccagcggatc catcccggcg
    27841 gcctcggcca gctcatccat catcatctcg acggcgaaca tcccctgcgg atgaccgggc
    27901 gcccgcatcg cgcgcggaaa cccgctgttc aggttcacat cctggtgcgt cttggccacg
    27961 atgccgaaat tgtagcgcga cgggttgctc gtgccgccgc cgccgccgac ggggccgacg
    28021 ctcccccacg tgtggatccg ccctcccagc agtttgccgt cgggtccgat cgcgagcttc
    28081 atgtattgca ggctgccggg gcgcaggccg ttgtcgaggt gctcttcctc gcgattcatc
    28141 agcacgcgca ccggccggcc gtgcttcttc gacagcttgg cggcgagctg cccctccggg
    28201 ctcagcccga acttgctgcc gaaaccgccg ccgacgtgtt cgcagtgcat ctccaccttg
    28261 tcggggggca ggcccagcac ctcggtcagc tcattgcgca ccgagaatgt cgcctgggtc
    28321 gactggcgcg cgatggcgtg atcgccgcgg tagtccacca ggctgttgtg cggctccagg
    28381 caggagtggg tctggacctg cgtctgatag gtgcgctcca gcgtatgggc ggcctcgctg
    28441 aagatccgct ccagatccgg ctgcttctcc tggacgctcg ggtcctcgtc gaacgtttcg
    28501 agcggctttt tttcgcgctc gatgtcggtc gccggccggt cgcgctcaaa ctccagcgcc
    28561 agcgcggcca gcccctgctc gagcgcctgg cgcgattcgg cgcagatgtg ccccagccgg
    28621 tcgccgggat aggtcccctc ctgcttctcc agctcgactt ccaccacgcc cggcgccgcg
    28681 cgcgccgcct ccagcttgct ggagcgcagg aaagccttgc catacgggca acggatgtag
    28741 ctcgcccaga gcagcccctc ggggtagtaa tcggtcgtgt agcgggcggc gccggtcacc
    28801 ttgtcgcgca catcgacgcg cgaaccgtcg ttttccagcg ccagtcgatt gtcagggcgc
    28861 cacgtcatca gccgttccct cccgccgcg
//
LOCUS       DAOPCO010000020        28656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1788_length_28656_cov_5.957973, whole genome shotgun sequence.
ACCESSION   DAOPCO010000020 DAOPCO010000000
VERSION     DAOPCO010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28656
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_28656_cov_5.957973"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLS90_02980"
     CDS             <1..217
                     /locus_tag="PLS90_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPK01400.1"
                     /translation="VTPEATATPTPAPGATPEPGAPEPIGADRADIPVVGQPEAAIAP
                     EGPRAADSVVTTRSLERAAPPAGEPGS"
     gene            334..1215
                     /locus_tag="PLS90_02985"
     CDS             334..1215
                     /locus_tag="PLS90_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HPK01401.1"
                     /translation="MKRQATHHPALRLLTALALFTSLATLHGAEPVPPEMRGVDIVEH
                     LGEKIPLDVRLTNEHGESVELGQYITGDKPVMLMLVYYSCPTLCNTMLNELTHGLKAM
                     QWTAGENFEILTVSIDPTETAELAAAKKANYLREYGRPEAARGWHWFVGDKANIDRLA
                     EAVGFKYKYDPVQRIYAHPPATYVISPAGVISRYLYGISYDPKTMKLALLEAGEGKIG
                     STLDRLILFCYHYDPSTRGYTLMALRIMRLGGGLTAVALFGMIGTYLWLERRRRRRMD
                     EASAAGPLEEPAAAAGK"
     gene            1320..2267
                     /gene="coxB"
                     /locus_tag="PLS90_02990"
     CDS             1320..2267
                     /gene="coxB"
                     /locus_tag="PLS90_02990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPK01402.1"
                     /translation="MDKTFFMPRQASTMAAAVDDVYYFVYWLSVFFFALIVGLMIFFV
                     IRYRRRSPDDVTPKITHSYALEAFWSIIPLFLIILIFAWGFKVYLQLRVVPANAFEIH
                     VTALKWQWQFTYPDGTVTIGELGVPVGRPVKLLMNSQDVIHSFYVPDMRVKQDVVPNR
                     YTVAWFEATEVGDHRIYCAEYCGDGHSQMYGWVKVMPPDVFNDWVAAGGIEAGNLPPV
                     EYGKLLFEKKTCIVCHSVDGTGNKQCPDLLGVFGHEVTLTDGSTVMADENYLRRSMME
                     PNADVVQGYRAEMPSFAGLLKDDQTTALIEYIKSLGASE"
     gene            2288..3931
                     /gene="ctaD"
                     /locus_tag="PLS90_02995"
     CDS             2288..3931
                     /gene="ctaD"
                     /locus_tag="PLS90_02995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HPK01403.1"
                     /translation="MSTATLPGPIPHDHDVGRINYLHSHRGVMSWVYTLDHKRIGIMY
                     LAAVTASFLVGGVMALLIRAELIDPQLTMLPSNKVYNQLFTLHGAIMVFLFIIPSIPA
                     ALGNFFLPIMLGAKDVAFPRLNLASFYIYVVGAIFAVISIVLGSVDTGWTFYTPYSVD
                     TQTSVVMMTMGAFILGFSSIFTGLNFIVTVHKLRAPGLTWFRMPLFVWALYATSIIQV
                     LATPVLGITLLLLAIERVAHVGIFDPLMGGDPVLFQHFFWFYSHPAVYIMIVPGMGII
                     SEIIPTFARKPIFGYRFIAFSSIAIALIGFLVWGHHMFTSGQSEIAVMIFSALTFLVS
                     IPSAIKVFNWTATLYRGSIDLKTPMLYALSFIFLFTIGGLTGLFLGALATDIHLHDTY
                     FIVAHFHYVMVGGTVMAFMGGLHYWWPKMTGRMYPELLGQITCGIVFLGFNMTFFTQF
                     ILGSQGMPRRYATYDEQFQPLHAFSSVGALVLGVGFAITTAYLLWSLWKGAKAPRNPW
                     GSLSLEWQIETPPIHQNFLETPVIEGMPYDYGVETGGGH"
     gene            4000..4701
                     /locus_tag="PLS90_03000"
     CDS             4000..4701
                     /locus_tag="PLS90_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HPK01404.1"
                     /translation="MSSAASAIDGHDHHHNPHLAHHFESMEQQHNSAKLGMWIFLVTE
                     ILLFSGLFLAYGAYRAFHPEVFNHASRLLSLPLGGFNTIVLIGSSLTMALAVREAQLG
                     RSRLQALFLLSTLALAGVFLVVKYFEYSHKFHVGLLPGRWFAPHGHEAHVAQWQNVRI
                     FFGIYFLMTGLHGIHVLMGMAAIGWLWLRARRGEFSAVYYTPVEVVGLYWHLVDIIWI
                     FLFPMLYLIDRSPMH"
     gene            4701..5264
                     /locus_tag="PLS90_03005"
     CDS             4701..5264
                     /locus_tag="PLS90_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HPK01405.1"
                     /translation="MADQSLHQPGHAGGAHAAHHVHVIPLKVYIGVWLALMFLTALTV
                     AVSFVHIGFLNVPIALSIAMIKATLVALIFMHLYYDEKFNLIVLLSGLVFVAIFFAFT
                     LIDPLTRGEVNADEGYFLVPEMPRAGVDVQPLAADGLTTGGHGEEDTHGAAGAMIDTH
                     QPGAEPAAVGGDASVPAEELSGEPAHP"
     gene            5261..6580
                     /gene="cyoE"
                     /locus_tag="PLS90_03010"
     CDS             5261..6580
                     /gene="cyoE"
                     /locus_tag="PLS90_03010"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HPK01406.1"
                     /translation="MIYRCPTLEPRGGGRLDAVWWATPVLLLSYLGVVCLTSPDAWRL
                     APAVTLFNGLLAAGLALALLALGGTWVAGLRAQHAESLLRPSTTFGFALFPALAGGLG
                     SWPGAAAALAGWGQALFLVALTLLLIAAVWHVGAGERQQRLLGAAEQRTGRPSWRDFY
                     ALTKMGLNALVLVTTSIGFYLASRGGIDGWLFLWALSGTALVAAGSSVLNQFIERDVD
                     ALMERTANRPLPAGRLRPFEALLFGTGLSLVGLLILIWRVNLLTAFLGGLTLAVYLFL
                     YTPMKRTSTLSTLAGAISGAVPPVMGWTATGEPIGPLAVVLFLILFFWQEPHFLAIGW
                     KYRKQYGAAGFPLYSVLDSRGKATAAQAVWYAAALLATTLAPLALGTGGAVYGTAAAL
                     AGIGFAALAIRFWFRPARGSAMALFFGSIAYLPLTLLLVLFLGGAQG"
     gene            complement(6990..8297)
                     /locus_tag="PLS90_03015"
     CDS             complement(6990..8297)
                     /locus_tag="PLS90_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01407.1"
                     /translation="MNRLNRILAALAVSLLVVTLTLLTWNRGFVSREIERDIASTVAS
                     FNPYEILHGVGDDMVRFGLLEQLAARALASPYIRNLVITRLVDGDRELPIIPWKMHAE
                     LGEGWRAAVDGWQRIALGGDDPFGYIYLDLDLSALRTMNWAIAAIGLTIAIMLATLLA
                     RVWRQETSLTRTVIELNERRREMIRLERLALAGQLAAGLLHDLRKPVLHIKHSLDDLD
                     EALGDFAPAAGSLQDLRRQTRLFFQILTDSQIERFVQSDKAVEEFVALAPIIEFSLNL
                     VRYEQRNVQVIRNEQEGLPPLLAHPFRLIQLFSNLILNAYQAMDGQGRLVIEAARAGR
                     GIEARIIDDGPGIPSDVIERVFDPFFTTKPEGEGTGLGLSISKMIVDDLGGTISVESR
                     AGGPTTFRIWLPAETAAEEPPAPRVPPASAPDTPPHPAPTPAD"
     gene            complement(8294..9520)
                     /locus_tag="PLS90_03020"
     CDS             complement(8294..9520)
                     /locus_tag="PLS90_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HPK01408.1"
                     /translation="MTPTHLPNPFPSSPRPTRAAPRRLLLLALLACIAFRAPLPAQPP
                     PADPPTGQRFVIGFLGEGSDFPNASVLMEELRERLLAEPRVREALDRAGFGPVVLSRG
                     GGPRDMLQLMGERELHLVFATAVVYASHLRPLLLDTPGVSSYQPILQTQRRDDFELPG
                     SSGVWRRGVVFAGPASALFHDDFSLRDDGARLAQELLAVPSDTSVSGYIYPRLMMERP
                     ELALPREFRFCGSDEEVVKHVVTGLLPIGACRQGELTSLLPNVDPDANPYYRILFKTN
                     PFPTDPILLRDDLLPERSDLGRELKAALREIFNGGLRPIAGDLRVENASARDFEDLSR
                     ALARFESISKDVSSRPATPPLVEPAPAEPTTPTPPADTTPTPAAEPTSQPTPAPTPVL
                     TPALPDLLLGLSGGEE"
     gene            9930..11165
                     /locus_tag="PLS90_03025"
     CDS             9930..11165
                     /locus_tag="PLS90_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPK01409.1"
                     /translation="MSEPPDKVSEPRGLGARARAWLQAVTRRRGAAATIRRARRLRGF
                     RHTTHVSGEGLLFVALALLIGLAAINTGTNLLYLILAIMISMLLHSFLLAWINLSRLR
                     MSRLYPLEFHAGETVQGQVELRNGKRLWGSYAIGVVDFVDGPANPELEESNLTLSAFG
                     VSVAPRGQIRCPVQFTLPQRGLYLLHHALLTSRYPFGLFERSVRFDEPAQLLVYPRLL
                     SEIAIAPHLPSVVGELAREKKGPGSGIHGIRAYREGDPQRQIHWKHSARGQGLMVKEM
                     EQEQTRSYRLMLDLRRPHEPAEDLLANLELAVSLAATLARMLLRQECRVGLWTSMGNV
                     PAGVGRQHLKRILRALAQVSSQPAGTTPVWPEGQLEDEAEIWIDYYTSDDRAARFGTI
                     IPRRPGCHVIDIRRLALEK"
     gene            11172..12224
                     /locus_tag="PLS90_03030"
     CDS             11172..12224
                     /locus_tag="PLS90_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPK01410.1"
                     /translation="MPPQVQDLLTEATRQGASDLHLVSWSPPIMRLNGTLVPMDMAQL
                     TPQDLAPMILGLLNEEQRNRFGRDWELDLSLTLRNVGRFRVNVHRQRGAIEAAFRIVT
                     DRIRTIRQLGLPPIIEELARRESGLILITGPTGSGKSTTLAAMIDQINQERRCMIVTI
                     EDPIEYVFRHRNSIIKQRELGIDTRSFAVALRESLRQDPDVIVVGEMRDLETIATALT
                     AAETGHLVLTTIHTPDVVQTVDRVVDVFPPHQQEQIRMQFANTIQGIIAQQLLPITGG
                     QGRVAACEVLVATLAVRKILRSAKAEQLHTALQTGTELGMITMDKSLKLLYQRGLISL
                     DMALAKARFPESFEHI"
     gene            12373..15654
                     /locus_tag="PLS90_03035"
     CDS             12373..15654
                     /locus_tag="PLS90_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01411.1"
                     /translation="MDQAGGGAGETAGPISGVSPGRPAHRRGVAWRRPALVVAVLGAG
                     LLLAVVAVGAALEDSHARMLLAVLAPPAAIVAAGGAAALRRSVRRWREVTAELRGMRE
                     RLQLFDGILSSVQRSIFILYNRQGDIVDAWGPADLATWFGLSLDQVRGRNMREFLLAE
                     HARRREQMLACVFEEGLPQSGPCEVQFQGKLFSYEVSISPLRDAAGRVTAAAAFCRET
                     NELRVAESRLYESREALRALLDAVDESAFLIDEDYRLLALNETAGRRLEQAPGELIGR
                     DVRPLWPADTAARRAAQIEWVRRTGEAVEFTDRRNGYVFENKLMPIRDERGRVVKIAI
                     FARDVSERQRAEEELRRSEERYRGIVESHVDLVVRLDLEERLAFVNDAFCRKFGYRRE
                     ELIGRRFLPQPHPDDVRLTLEVLRDLRFPPYRTHHQARMMTPEGWRWIAWENTTIFDR
                     AGRIEEFQGIGRDVTDYVNSLDALRQSEERYKSIVENTRDLIIVSEPGGAISYASPAC
                     EQVLGWSSAALIAGEVTGIPPAPGKPCFVHPEDRERVVAFLSGQAGDPGARTLEYRIV
                     TRDVDVRWVSHAWSPVCCGGKPRQVIHIVRDVTPMRMAEQAERRRSQDLARVYENVPI
                     GLWKLSMDRTRILAFSPLMTEITGLDMNVMFADPTDWVERFGDNPESRRAMQIMDERI
                     AQQLPFDVEYEFRTESRGPRWLQSIAKPATEHGEVVYYGVTLDITERRAMERERNLLA
                     EALNQADEMIIVTDAAGRIVYVNPAFERITGYRREEALGQTPRLIKSGHHHPSHYQGL
                     WGTIREGRVWRGHFINRRRNGEMFEQFATITPVLNDQRQVTHFIGVQRDISIERELER
                     RLRHAQRISDIGQTIMGVSHHLKNILSGLRGSSMLMERGLETGDLCRVRELWPVFQRN
                     TERMSRLADEMLNYARLQRLELAPVDPGELSGEVLENQRSLAEQRRVSLEAELAEPLP
                     RLLCDRNRMYDALLNLVCNAVDACDDVGGGRVGIQVRYLEQERQIEICVHDNGRGIDE
                     ETLARIFNPFFTTKGSKGTGLGLAVVQKTVHEHGGRIEVTSRPGRTCFTMWIPVDGRP
                     GGKEE"
     gene            complement(15763..16734)
                     /locus_tag="PLS90_03040"
     CDS             complement(15763..16734)
                     /locus_tag="PLS90_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007042350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HPK01412.1"
                     /translation="MKAPWVTYRPEIRVMDCTVRDGGLINDHRFEDGFVRAVYDTCVA
                     AGIDAMEIGYKASDRLFARDAFGAWKFCREDDLRRVVGDNPSALRLSAMADAGGKTDW
                     KTDILPKDQSVLDIIRVACYINQIPEAVEMIKDAHDKGYTTTCNIMAISQVQETELDQ
                     ALDVLAQTPVDVVVVVDSFGALHSEQVQLLVRKFLAAVKPAGKEVGIHAHNNLQLGFA
                     NTIEAIIQGANRIDATMAGLGRGAGNCPMELLLGFLRNPKFKIRPVWQLIEEQMIPLR
                     NRIEWGPLPAYIITGQLNQHPRAAINWRAGDRRDHCAEFYDRCISDV"
     gene            complement(16878..18020)
                     /locus_tag="PLS90_03045"
     CDS             complement(16878..18020)
                     /locus_tag="PLS90_03045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01413.1"
                     /translation="MPPSPLPVLFMVQHLDSGGTEHHFHDLVTRLDRDLIAPHVIYCE
                     PGIVSRQLEALDWLPVTRIPLTRAYDWSGLRAALRVRRYLRRHAIGAVVAFHFVADFL
                     AVLAAWPGPLPVISSRRDMGFTRTSRQIRIGRWMQPRIHRYIAVSGAVAEAVARDEAV
                     PPGKISVIHNGIDLDRLHREKGDAAAERARLGIAPGELVIGCAATFNPVKRHDLLLEA
                     FARLRRAGGPPVRLLLAGDGPLAGEIAARRRALGLERAVLMVGRSPRVARELNAADIV
                     ALASETEGFSNAIIQAMALGRPVVACRVGGNPEAVHDGATGLLVPPNDPAAFAAALER
                     LVADEPLRREMGRRAAELAAARFSLQAMVERTQSLILAAARPAALE"
     gene            complement(18051..21971)
                     /locus_tag="PLS90_03050"
     CDS             complement(18051..21971)
                     /locus_tag="PLS90_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPK01414.1"
                     /translation="MTEAAAPATPAPRWTPAQRAAIESRAGNLVVSASAGAGKTTVLT
                     ERVLRLITEPGPAGEPPARIDQMLIITFTEKAARQMRERIERRIHQGLAAAPDNPRLL
                     DALDRLGGAWIMTIDAFCRRVVVDHFHRAGLAPNPRVPDEAERAQLELAVLDELLEER
                     AAAPGPPRAELEWLLACQPGGARALAAELRELLAFLQSLDRPDLWLRRARERIDAVER
                     AQTFADLPEAAAARAAFADSTAALTRALCDLIDLAGARGGDPACLDAWRWIAHGLDAL
                     ARREPLQLDAARDWLAQFADGTLDKLQNKTNCGDALYQNEAFRKHTLERFARRYAAWR
                     DKWFALDEAGLLHGARLAARQARALLQLADEARRAIDAALRRRGLATFNDFERIAFDV
                     LCDPATDAPSDIARAYQEQFRYVLVDEYQDTSPLQDALIRRVTRLDDPSPNAEGNLFI
                     VGDYKQSIYRFRHAEPGLFLDKLRAAEREAVPGIRRVVRIDLPDNFRARRPLIEFVNH
                     CFERLMDEQVGELRYADRERLVHGRPDELESGEPAVEVHWLPRAAEPPAPEAAGEEDS
                     SVRAEEELTGVEAQAAWVARRIRELTDPAGALRIPTGDGAWRPAVPGDCAILLRSLMN
                     EIDVWSDALEREGLAVRAPGLSPLFTAPELIDLVHALRAIDNPLQDVPLAALMRSPMF
                     AFSDDDLLRLRLARRKQPFSAAVMDLCADPDAADLPADLRRRLRAFGDTLAAWRRLAW
                     SNTPVELLAAILRDTRYEAWLRGKPDAAERLQRVDYLRDLLARLGHAADGANPLAAFL
                     ELLERAESESLELGELPESMVEQHRAVSLLTIHKSKGLEFPIVFLPRLERGFRTARPR
                     ELHCDRLHGLALRGVDLAGRRRYGTLALRVMEEAALRQERSEELRLLYVAMTRARERL
                     VLVGQAPDPQSLPEQWGALRQTGGRPLGVLDRLTARSPADLLGPIIEWLRAPDGGGAP
                     PWLTVRLEDELPRRAEKDDALFVREALAHPGPPAARAAACRTAAQRLDELARAPAADN
                     GTPLSPAFVPPHDPLAPLTRVPAKATVTQLRRLRDEAERAAELDRVDRLAAAGEIDWN
                     EASRFIEEEMQRPAFARAGALAARRPAWCDPPTHRPTGAVRGTLTHALLAQLDLTGDL
                     SPDGIRRQARDLAARGRLELAAGADELDGLDYDALGWFFSTEPGRRMRERPLSVRREL
                     PFTAARDATEFSPAAARAGLRETVLLQGIIDVILDDGDAATVLDYKTDWTDGGRRVAE
                     LREHYRLQIECYGRALRAVWRLQRVQAALVFLDARAIEWISLDD"
     gene            complement(21964..25578)
                     /locus_tag="PLS90_03055"
     CDS             complement(21964..25578)
                     /locus_tag="PLS90_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPK01415.1"
                     /translation="MKRRWSTVKTCEIWLGRAGAGKTWGCLSAIADELRAAPRGPLLG
                     LLVPEQATAQMERRLLRMPGVENGFTRARVFSFNHLAREALARRGAPPARRVGEAGRL
                     MLLRRALESLRPRLLVFQTTAGQPGVAESVHEALLELQRYGWRPDDLEARLAAMPPAA
                     RQTDPSARKLADLALIWRDYERLLAELGLEDMLAFYPAAAQAIAQWPDLHGAHLWIDG
                     FSSFTTLEWRLVEAVTRQAASLVLALACDPIEFLGTGGDRPHPVGPQRLFHSTLQTAR
                     QAAERFRELDWHVEIIALPEPDQPTRFAASPALAHIERTVPAQLLPRALDDADWQRSA
                     RAAAELLPAAPADAWTPAPIEIIEARDRRAEIEAIARRIVALCRVDRPAAHGPRPLTW
                     RDIGLIARDLTPYQHLIPEVFRQFEIPFFLDRPRQIEGHPLCRLILAALRLLRFRGRG
                     REVVHYLKTGLSGLDDPLHVAELENLILALDPDGRRWLKIAADGPPWMREIADRWRRA
                     AAPLRRLEETLRAGDPPARALWRLLDDVRAAATLEAWIERDRRQGDEESALLHEQAWA
                     QVIEWLEELDRLPPAPAPADADPMQAYYARLDDLALLADTALRATRARLIPPTLNQVT
                     VGAVDRSRTPELKLAFLIGLNEQEFPRAWNPDPILGDAERDLLTRDGGAIGPDLQARR
                     SQEHYLGYIALTRASHALVLTRPLLDDAGKAGDPSPFLRMVRAAAPQAPASLVDRDAR
                     DERPGLPLRAEEWMSRLCGLLADLDLGPARAQLAAALRAGHPLDRDDLAPPTREALRV
                     GFSALAPPARPALDPDLAAAVWEPGARVRVTALERFAACPFSFFAEQVLRLQDRETAE
                     LQPVDLGSIRHDLLQRVFHDLRGTAPTLAWGEIDPAAADAAIERRLEQLRSNPRWSRR
                     LAARALNDLTLAQLAAELKLFIRALRLAGAHCAFRQAHAEWQFGEAGELDVRLADGAS
                     FSIVGKVDRIDQLADADGRFAWLIFDYKSGRHVLDISRLMAGLDLQLAVYGLAFQQAM
                     HRRGIPGRIAGIFYWPLAAPLHDAQAGDAAQCDPVDPRWFERLAPRGIFNEEFAATLD
                     RSVGPGEASVAFGFRRASKGQLHGRGQAHWPAIAFDRLLRDERRLLRDLLARIARGEI
                     AATPFRHRSETACRHCAFHPLCRRFEPGAVQPVTIPPMDRARIIDRFNVEPECEAGDD
                     "
     gene            25893..26606
                     /locus_tag="PLS90_03060"
     CDS             25893..26606
                     /locus_tag="PLS90_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPK01416.1"
                     /translation="MSWTLKHYRGQRVQPTGMGLLGLVGLSLSLGCATAPPPARVATV
                     TTARRPPASPVRVFIPKVMVSPEENGKPVYEKVEDYGDKYPETTAPPEQGTIEMLSHD
                     DPLLSEFEELRIRGEAWPMVANRGASQVYPPEEGVVSFYWQAQRLASGDYFDPGKLCA
                     AHKTLPFGTIVRCTRLDNGQYVDVIINDRGPYIKGRVLDLSKAAASRIGLIGRGIARC
                     RIEVLAYPLIETMGPNGNG"
     gene            complement(26616..28409)
                     /locus_tag="PLS90_03065"
     CDS             complement(26616..28409)
                     /locus_tag="PLS90_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK01417.1"
                     /translation="MRPLSFLLLAAPLVILAACATPPIELPTYEVDTQRLLQTPPAPV
                     AAAPSDRASHVVIDDVRITPETAVGYDDLPASITQLFDSSPLDLSVADVVIETLANNH
                     EIQIQGYSLRIDEYEIPVQKGIYDLMLNARAEFSRIEEQTSTSSFASTGINSTRRRQF
                     LTGLSQLFPTGATLDLGYAIARSTNLFSSISLAFLPGIVSPDNPLGLPVPMFSSTTRT
                     DVQYRQSFTAALTQPLLRGFGPAVTNANIRIAQLERQGSAADFQTQVEETLLLALETY
                     WQLIEAIELHKVAVVSYSAAADLLRVNRARYDAGVLPLTDVLQAEAAVGGRRVDVINA
                     RRSVRELEDRLKQILLLQPDAPLWEAQLRPSQPFAWREIDVDLEESIDVALDRRAEIR
                     RATSNIDQADVQLQVARNNLLPRLDLIGEVESSGLDDDGTGAYRTAKDGKYISYTAAL
                     QFSYPLQNRAARYRHRQARAQAEMAGENLQAVVDRVALDVRNAVRLLRTARERIDVTQ
                     SQVRAAQALLDAELKRLDVGVSTAFQVLQFQEDLAEAQANHLRAVVDYNRAAIRLERA
                     RGTLLDTYGVHVVGAELMPAVEPVVFPIGFN"
BASE COUNT         4354 a         8127 c        11607 g         4568 t
ORIGIN      
        1 ggtgacgccc gaggcgacgg cgacgccgac gcccgcgccc ggcgcgacac ccgaaccggg
       61 cgcgcccgag ccgatcggcg ccgaccgcgc cgatatcccg gtcgtgggtc agcccgaagc
      121 ggcgatcgcg ccggaggggc cgcgagcggc cgattcggtg gtgacgacgc ggtcgttgga
      181 acgcgccgcg ccgccggcgg gcgagccggg gagttaggga gagaccttcg ggggcgatta
      241 cgagggacga gagacgaatt tagagcgggg acgcgagacg acatttgacg gtgggaagga
      301 tggcgagacc cgcgggggga aggaaccccg atcatgaaac gacaggcgac acatcacccg
      361 gccttgaggc tgctgaccgc gctggctctg ttcacatccc tggcaaccct tcatggcgct
      421 gagcccgttc cacccgaaat gcggggggtg gatatcgtcg agcacctggg cgaaaaaatt
      481 ccgctggacg tgcggctgac gaacgagcac ggcgagtcgg tcgagctcgg gcagtacatc
      541 accggggaca agccggtgat gctgatgctg gtgtattact cctgcccgac gctgtgcaac
      601 acgatgctga acgagctgac gcacgggctg aaggcgatgc aatggaccgc gggggagaac
      661 ttcgagatcc tgacggtgag catcgatcca acggagacgg cggagctggc ggcggccaag
      721 aaggcgaact acctgcggga atacggtcgg ccggaggcgg cgcgcgggtg gcactggttc
      781 gtgggggaca aggcgaatat cgaccggctg gccgaggcgg tcggcttcaa gtacaagtac
      841 gatccggtcc agcggatcta cgcgcatccg ccggcgacgt atgtgatctc gccggcgggg
      901 gtgatctcgc gctacctcta cgggatctcg tatgatccga agacgatgaa gctggcgctg
      961 ctggaggcgg gggaagggaa gatcgggagc acgctggatc gactgatcct gttctgttac
     1021 cattatgatc cttccacgcg cggctacacg ctgatggcat tgcggatcat gcggctgggg
     1081 ggcggcctga cggcggtcgc gctattcggc atgatcggga cgtatctgtg gctggagcgg
     1141 cgacggcgcc ggcggatgga cgaggcatcg gcggcgggtc cgctggagga gcccgcggcg
     1201 gcggcgggga agtaggggcc gcggcggggg cgaaggtgtg cgggggggac gcgtagcgcc
     1261 ccggaagttg ataacctgat agggatatgg agatcgccgc cgcgcgcggc cggagtatca
     1321 tggacaaaac cttcttcatg ccccggcagg cctcgacgat ggccgccgcc gtcgatgacg
     1381 tctattactt cgtctactgg ctgtcggtgt ttttctttgc gctgatcgtc gggctgatga
     1441 tcttctttgt catccgctac cggcggcgca gccccgacga cgtgacgccg aagatcacgc
     1501 acagctatgc gctcgaggcg ttctggagca tcattccgct gttcctgatc attctgatct
     1561 tcgcctgggg gttcaaagtt tatctgcaat tgcgcgtcgt gccggccaat gcatttgaga
     1621 ttcacgtgac cgcgctgaag tggcaatggc agttcaccta tcccgacggg acggtgacga
     1681 tcggcgagct gggtgtgccg gtgggccggc cggtgaagct gctgatgaac tcgcaggacg
     1741 tgatccacag tttctatgtg cccgacatgc gggtgaagca ggacgtggtg cccaaccgct
     1801 acacggtggc ctggttcgag gcgacggagg tgggggatca ccggatctac tgcgccgagt
     1861 attgcggcga cgggcactcg cagatgtatg gctgggtcaa ggtgatgccg cccgacgtgt
     1921 tcaacgactg ggttgcggcg gggggcatcg aggcgggcaa cctgccgccg gtggagtacg
     1981 gcaagctgct gttcgagaag aagacctgca tcgtctgcca ttcggtcgac ggcacgggca
     2041 acaagcagtg ccccgacctg ctgggggtgt tcgggcacga ggtgacgctg accgacggct
     2101 cgacggtgat ggccgacgaa aattatctgc gccgctcgat gatggaaccc aacgccgacg
     2161 tggtgcaggg ttaccgggcg gagatgccga gtttcgccgg gctgctgaag gatgaccaga
     2221 cgacggcgct gattgaatac atcaagtcgc tcggggcgag cgagtaatcc gcgaggagac
     2281 gaggacgatg tcgaccgcaa cgctacccgg cccgatcccg cacgaccacg acgtcgggcg
     2341 gatcaattac ctgcactcgc accgcggggt gatgtcgtgg gtctacacgc tggaccacaa
     2401 gcggatcggg atcatgtatc tggcggcggt gaccgcctcg ttcctggtgg ggggcgtgat
     2461 ggcgctgctg atccgggccg agctgatcga cccgcagctg acgatgctgc cgagcaacaa
     2521 ggtttacaat cagctcttca cgctgcacgg ggcgatcatg gtgttcctgt tcatcatccc
     2581 gtcgattccg gcggcgctgg ggaacttttt tctgccgatt atgctcgggg cgaaggacgt
     2641 cgcgttcccg cggctgaacc tggccagttt ctacatctac gtggtcgggg cgatctttgc
     2701 ggtgatttcg atcgtgctgg ggtcggtcga cacgggttgg acgttttaca cgccgtacag
     2761 cgtggatacg cagacgagcg tggtgatgat gacgatgggg gcgttcattc tggggttttc
     2821 gtcgatcttc acggggctca atttcatcgt cacggtgcac aagctgcggg cgccggggct
     2881 gacgtggttc cggatgccgc tgttcgtgtg ggcgctgtat gcgacgtcga tcatccaggt
     2941 gctggccacg ccggtgctgg ggatcacgct gctgctgctg gcgatcgagc gggtggcgca
     3001 cgtggggatt ttcgatccgc tgatgggcgg cgacccggtg ctgtttcagc atttcttctg
     3061 gttctactcg catccggcag tctacatcat gatcgttccg gggatgggga tcatcagcga
     3121 gatcatcccg acgttcgcgc gcaagccgat cttcggctac cggttcatcg cgttctcatc
     3181 gatcgcgatc gcgctgatcg ggttcctggt atggggccat cacatgttca cgagcgggca
     3241 gtcggagatc gcggtgatga tcttttcggc gctgacgttc ctggtgtcga ttccgtcggc
     3301 gatcaaggtg ttcaactgga cggcgacgct gtatcgcggc tcgatcgatc tgaagacgcc
     3361 gatgctctac gcgctatcgt tcatcttcct gttcacgatc ggcgggctga ccgggctgtt
     3421 tctgggggcg ctggcgaccg acatccacct gcacgacacg tatttcatcg tcgcgcactt
     3481 ccattacgtg atggtcggcg ggacggtgat ggcgttcatg ggggggctgc attactggtg
     3541 gccgaagatg acggggcgga tgtatccgga gctgctcggg cagatcacgt gtgggatcgt
     3601 cttcctggga ttcaacatga cgtttttcac gcagttcatc ctgggcagcc agggaatgcc
     3661 gcggcgctac gcgacgtatg acgagcagtt ccagccgctg catgcgttct cgtcggttgg
     3721 cgcgctggtg ctgggcgtgg gattcgccat cacgacggcg tatctgctgt ggtcgctgtg
     3781 gaagggggcc aaggcgccgc gcaatccgtg gggctcgctc tcgctggaat ggcagatcga
     3841 aacgccgccg attcaccaga atttcctgga gacgccggtg atcgagggga tgccgtatga
     3901 ttacggggtg gagacgggcg gcgggcattg agggttcgag agccgatgac gagggacgag
     3961 gacgagggac gaactaatca tgactgaggg caacgagcga tgagcagcgc cgcgagtgcg
     4021 atcgacgggc acgatcacca ccacaacccg cacctggccc atcatttcga atcgatggag
     4081 cagcagcaca actcggccaa gctggggatg tggatctttc tggtcacgga aatcctgctg
     4141 ttcagcgggc tgttcctggc ctatggggcg tatcgcgcgt tccatccgga agttttcaac
     4201 cacgccagcc ggctgctgag cctgccgctg gggggcttca acacgatcgt gctgatcggc
     4261 agcagcctga cgatggcgct ggcggtgcgg gaggcgcagc tcgggcggag ccggctgcag
     4321 gcgctgttcc tgctcagcac gctggcgctg gcgggggtgt tcctggtcgt caagtatttc
     4381 gaatattcgc acaagtttca cgtcggtctg ctgccgggcc ggtggttcgc gccgcacgga
     4441 cacgaggcgc acgtggcgca gtggcagaat gtgcggatct ttttcggaat ctactttctg
     4501 atgaccggcc tgcacgggat ccacgtgctg atggggatgg cggcgatcgg ctggctgtgg
     4561 ctgcgcgcgc ggcgagggga gttcagcgcc gtatactata cgccggtcga agtcgtcggg
     4621 ctctactggc acctggtcga tatcatctgg atcttcctgt tcccgatgct ctacctcatt
     4681 gacaggtcac cgatgcactg atggcggatc agagtttgca ccaaccgggg cacgcaggcg
     4741 gcgcgcacgc cgcgcatcac gtccatgtga tcccgctcaa ggtctacatc ggcgtgtggc
     4801 tggcgctgat gttcctgacg gcgctgacgg tggccgtgtc gttcgtacac atcggctttc
     4861 tgaacgtgcc gatcgcgctg agcatcgcga tgatcaaggc gacgctggtg gcgctgatct
     4921 tcatgcatct gtattacgac gagaagttca atctgatcgt gctgctgagc gggctggttt
     4981 tcgttgcgat cttttttgcg ttcacgctga tcgatccgct gacgcgcggc gaggtcaacg
     5041 ccgatgaagg gtattttctg gtgcccgaga tgccgcgcgc gggggtggac gtgcagccgc
     5101 tggcggccga cggattgacc accggcggcc acggcgaaga ggacacgcac ggcgccgccg
     5161 gggcaatgat cgacacgcat cagcccgggg ccgagccggc ggcggtgggg ggcgacgcga
     5221 gcgttcccgc cgaggagctg agcggagagc ccgcacatcc atgatttatc gttgtcccac
     5281 gctcgaaccg cgcggcgggg ggcggctgga cgccgtctgg tgggcaacgc ccgtgctgct
     5341 gctgagctat ctgggggtgg tgtgcctgac gtcgcccgat gcgtggcggc tggcgccggc
     5401 ggtgacgctg ttcaacgggc tgctggcggc ggggctggcg ctggcgctgc tggcgctggg
     5461 ggggacgtgg gtcgcgggcc tgcgggcgca gcatgccgag tcgctgctgc ggccgtcgac
     5521 gacgttcggt ttcgcgctgt tcccggcact ggcgggcggg ctgggatcgt ggcccggcgc
     5581 ggcggcggcg ctggccggct ggggccaggc gctgtttctg gtcgcgttga cgctgctgct
     5641 gatcgcggcg gtgtggcatg tcggcgcggg ggagcggcaa cagcggctgc tgggcgccgc
     5701 cgagcaacgg acgggccggc cgagctggcg cgatttctac gcgctgacca agatgggcct
     5761 gaacgcgctg gtgctggtga cgacgtcgat cggattttat ctggcctcgc gcggcgggat
     5821 cgacgggtgg ctgttcctgt gggcgctgag cgggaccgcg ctggttgcgg ccggctcgag
     5881 cgtgctgaac cagttcatcg agcgcgatgt ggatgcgctg atggagcgaa cggcgaaccg
     5941 gccgttgccc gccggccggc tgcggccgtt cgaggcgctg ctgttcggga ccgggctgag
     6001 cctggtcggc ctgctgatcc tgatctggcg ggtaaacctg ctgacggcgt ttctgggagg
     6061 gctgacgctg gcggtctacc tgtttctcta cacgccgatg aaacggacaa gcacgctgtc
     6121 gacgctggcg ggggcgattt cgggggcggt gccgccggtg atgggctgga cggcgaccgg
     6181 cgagccgatc gggccgctgg ccgtcgtgct gttcctgatt ctgttcttct ggcaggagcc
     6241 gcattttctg gcgatcggct ggaagtaccg caagcaatac ggcgcggcgg ggttcccgct
     6301 ctacagcgtg ctggattcgc gcggcaaggc gacggcggcg caggcggtgt ggtatgccgc
     6361 ggcgctgctg gcgacgacgc tggcgccgct ggcgctgggg acagggggcg cggtctacgg
     6421 gacggcggcg gcgctggccg gcatcgggtt cgccgcgctg gcgatccggt tctggttccg
     6481 tccggcgcgg ggcagcgcga tggcgctgtt tttcgggtcg attgcgtatc tgccgttgac
     6541 gttgttattg gtgctgtttc tggggggcgc gcaggggtga gggagcgggg gcaggtgcgg
     6601 cgcgccgccc ggggttgatt gagttctggc ttgcccattg ataccgattc acacggattg
     6661 acgcggacat ctggcatcgc tccgggatgc gggggggagc ggcggggcgc cggcgccata
     6721 ccagtggaat tcggttcggt cgccatccgg gcaccgcttg caggaggttg gggcgcgggg
     6781 ggcggggacg cagggttccg gcctgcggcc tccactctgc gcttgatgcc ggtcgtccgc
     6841 atcgcggatt tgggcattcg ccccctgcgg ggacgggggg ggggtggaac gggctgtccc
     6901 caggttgcgc tgcgctcctc ctggagttat tcaaaatccg cctcttcgag gcggcagagg
     6961 gcatcccgcc aggctgcggg aggacggact cagtcggcgg gggtcggtgc ggggtgcggc
     7021 ggggtgtcgg gggcggatgc gggcgggacg cgcggagcgg ggggttcttc ggcggcggtc
     7081 tccgcgggga gccagatgcg gaacgtggtg gggccgccgg cgcggctttc gaccgagatc
     7141 gtgccgccga ggtcatcgac gatcatcttg ctgatcgaga gacccaggcc ggtgccctcg
     7201 ccctcgggtt tggtggtgaa gaacgggtcg aagacgcgct cgatgacgtc ggacgggatg
     7261 cccgggccgt cgtcgatgat gcgggcctcg atgccgcggc cggcgcgggc cgcttcgatg
     7321 acgaggcggc cctggccgtc catcgcctgg taggcgttca ggatcaggtt ggagaagagc
     7381 tggatcaggc ggaagggatg cgcgaggagc ggggggaggc cctcctgctc gttgcggatg
     7441 acctggacgt tgcgctgttc gtaacggacg aggttgagcg agaactcgat gattggggcg
     7501 agggcgacga actcctcgac ggccttgtcg gactggacga agcgttcgat ctggctgtcg
     7561 gtcaggatct ggaagaagag gcgggtctgg cggcggaggt cctgcaggct gccggcggcg
     7621 ggggcgaagt cgccgagggc ctcgtcgagg tcatcgagcg agtgcttgat gtggaggacc
     7681 ggcttgcgca gatcgtggag caggccggcg gcgagttggc cggcgagggc gaggcgctcc
     7741 aggcggatca tttcgcggcg gcgctcgttg agctcgatga cggtgcgggt gagggaagtt
     7801 tcctggcgcc agacgcgggc gaggagcgtg gcgagcatga tcgcgatggt gaggccgatg
     7861 gcggcgatcg cccagttcat cgtgcgcagg gcggagagat cgagatcgag atagatgtag
     7921 ccgaagggat cgtcgccgcc gagggcgatg cgttgccagc cgtcgacggc cgcgcgccag
     7981 ccctcgccca gttcggcgtg cattttccag gggatgatcg gcagctcgcg gtcgccgtcg
     8041 acgaggcgcg tgatcaccag attgcggatg taggggctgg ccagggcgcg ggcggcgagc
     8101 tgctcgagca ggccgaagcg gaccatgtcg tcgccgacgc cgtggaggat ttcgtagggg
     8161 ttgaacgagg cgacggtgct ggcgatgtcg cgctcgatct cgcggctgac gaaaccgcgg
     8221 ttccaggtca gcagcgtgag cgtgacgacg aggagcgaaa cggccagggc ggcgaggatg
     8281 cggttgaggc ggttcattcc tcgcctccgc tcaggccaag gagcaaatcg gggagcgccg
     8341 gggtcaggac gggcgtgggc gcgggggtcg gctgggaggt cggttccgcg gccggcgtgg
     8401 gggttgtgtc ggcgggcggc gtgggggttg tgggctccgc gggcgccggc tcgaccaggg
     8461 gcggcgtggc gggtcggctg ctgacatcct tgctgatcga ttcgaagcgg gcgagggcgc
     8521 gcgagaggtc ctcgaagtcg cgggcggagg cgttttcgac gcgcagatcg ccggcgatgg
     8581 gccggaggcc gccgttgaag atctcgcgca aggcggcctt gagttcgcgc cccaggtcgg
     8641 agcgctcggg gagcaggtcg tcgcgcagga ggatcgggtc ggtcgggaag gggttggtct
     8701 tgaagaggat gcggtagtag gggttggcgt cgggatcgac gttggggagg agggaggtca
     8761 gttcgccctg gcggcaggcg ccgatgggga gcaggccggt gacgacatgc ttgacgacct
     8821 cctcgtcgga gccgcagaag cggaactcgc gggggagggc cagttcgggg cgctccatca
     8881 tcagccgggg gtagatgtag cccgagaccg aggtgtcgga ggggacggcg aggagttcct
     8941 gggcgaggcg ggcgccgtcg tcgcgcagcg agaagtcgtc gtggaagagg gcggaggcgg
     9001 gcccggcgaa gacgacgccg cggcgccaga cgccgctgga gccgggcaat tcgaaatcgt
     9061 cgcgccgctg ggtctgaagg atcggctgat agctgctgac gccgggggta tcgagcagga
     9121 gcgggcggag gtggctggcg tagacgacgg cggtggcgaa gacgagatgg agttcgcgtt
     9181 cgcccatcag ctggagcatg tcgcgcggtc cgccgccgcg ggagaggacg acggggccga
     9241 agccggcgcg atcgagcgcc tcgcggacgc gcggctcggc gaggagccgc tcgcgcagtt
     9301 cctccatcag gaccgaggca ttggggaaat cggagccttc gccgaggaaa ccgatgacaa
     9361 agcgctggcc ggtcgggggg tcggccgggg gcggctgggc gggcaggggg gcgcggaaag
     9421 cgatgcaagc cagcagggcg agcaagagca ggcggcgcgg cgcggcgcgg gtcgggcgcg
     9481 gggaggaagg aaaggggttg ggaagatgag ttggggtcat gtcgggtgat acgtcaatcg
     9541 tgtccggggg gcgtcgcggg attccaattc gccccgagcg gggacggaga ttcttggggt
     9601 gtcctccacc ccaggttccg ctacgctccc cccgggctat tcctattcca cccctgccgg
     9661 ggcggtggca gcgcggcgcc ggtcccacat cccatacgca accgggggga ggcgtggcgg
     9721 ctctgggaag atgagggggc agcggcaagg cgcgcatcct ggatcggccg gcggagcggc
     9781 agccggcgtg ggcgttggac gaagggagcg aaccgcaccc gttaatcgat agcaatcccg
     9841 attcgtcttg ggaacaaaaa acgattatca tggcggctcg gttggtggaa tcgcccggcc
     9901 gagcgagccc ggaatcagga cgcgtggtgt tgtcggaacc gccggacaag gtcagcgagc
     9961 cgcgggggct gggcgcgcgc gcgcgggcgt ggctgcaagc cgtgacgcgg cggcggggcg
    10021 cggcggcgac catccggcgg gcgcggcggt tgcgcggatt ccgccacacg acgcacgttt
    10081 cgggcgaggg gctgttgttc gtcgcgctgg cgctgctgat cggcctggcc gcgatcaaca
    10141 ccggcaccaa cctgctgtat ctgatcctgg cgatcatgat ttcgatgctg ctccattcgt
    10201 tcctgctggc gtggatcaac ctgtcgcggc tgcggatgag ccggctgtat ccgctcgaat
    10261 ttcacgcggg ggagacggtg caggggcagg ttgaactgcg caacggcaaa cggctgtggg
    10321 ggagctatgc gatcggcgtc gtggatttcg tggacgggcc ggccaatccg gagctggagg
    10381 agagcaacct gacgctgagc gcgttcgggg tgagcgtggc gccgcggggc cagatccgct
    10441 gcccggtgca gttcacgctg ccccagcgcg ggctctacct gctgcaccac gcgttgctga
    10501 cgtcgcgcta cccgttcggg ctgttcgaac ggagcgtgcg gtttgacgag ccggcgcagt
    10561 tgctggttta cccgcggctg ctgtcggaga tcgcgatcgc gccgcacctg ccgagcgtgg
    10621 tgggcgagct ggcccgcgag aagaagggcc ccggcagcgg gatccacggg atccgggcct
    10681 accgcgaggg ggacccgcag cgccagattc actggaagca ttcggcgcgc ggccagggcc
    10741 tgatggtgaa ggagatggag caggagcaga cgcgcagcta ccggctgatg ctggacctgc
    10801 gccggccgca cgagccggcg gaggatctgc tggcgaacct ggagctggcg gtgagcctgg
    10861 cggcgacgct ggcgcggatg ctgttgcgcc aggaatgccg ggtggggctg tggacgtcga
    10921 tggggaatgt gccggcgggg gtggggcggc agcatctgaa gcggatcctg cgggcgttgg
    10981 cgcaggtctc gtcgcagccg gcggggacga cgccggtgtg gccggagggc cagctggagg
    11041 atgaagcaga gatctggatt gattactata cgtcggacga ccgggccgcg cggttcggga
    11101 cgatcattcc ccggcggccg gggtgccatg tgatcgacat ccgccgtctg gcgctggaga
    11161 agtagccgcc gatgcccccc caagtacagg atctattgac ggaggcgacg cgccagggcg
    11221 cgtcggacct gcatctggtg tcgtggagtc cgccgatcat gcggctcaac gggacgctgg
    11281 tgccgatgga catggcgcag ctgacgccgc aggatctggc gccgatgatc ctggggctgc
    11341 tcaatgagga acagcgcaac cggttcggtc gggactggga gctggacctg tcgctgacgc
    11401 tgcgcaacgt gggtcggttc cgggtgaacg tgcaccgcca gcggggagcg atcgaggcgg
    11461 cgtttcggat cgtgacggac cggatccgga cgatccgtca gctcgggctg ccgccgatca
    11521 tcgaggagct ggcgcggcgc gagtcggggc tgatcctgat cacggggccg accggatcgg
    11581 gaaagtcgac gacgctggcg gcgatgatcg accagatcaa tcaggaacgg cggtgcatga
    11641 tcgtaacgat cgaggatccg atcgagtatg tgttccgcca tcgcaacagc atcatcaagc
    11701 agcgcgagct ggggatcgac acgcggagtt tcgcggtggc gctgcgcgaa tcgttgcggc
    11761 aggaccccga cgtgatcgtc gtcggcgaga tgcgcgacct ggagacgatc gcgacggcgc
    11821 tgacggcggc cgagacgggg cacctggtgc tgacgacgat ccatacgccg gacgtggtgc
    11881 agacggtgga ccgggtggtg gacgtttttc cgccgcatca gcaggagcag atccggatgc
    11941 agttcgccaa cacgatccag gggatcatcg cgcagcagtt gctgccgatc acggggggcc
    12001 aggggcgggt ggcggcgtgc gaggtgctgg tggcgacgct ggcggtgcgc aagatcctgc
    12061 ggtcggcgaa ggccgagcaa ctgcacacgg cgctgcagac ggggaccgag ctggggatga
    12121 tcacgatgga caagtcgttg aagctgcttt accagcgggg gttgatctca ctcgacatgg
    12181 cgctggcgaa agcgcggttt ccggagagtt tcgagcatat ctgaggaggg gagggcaggg
    12241 cgaggacgag ggacgattat gtaggacata ttcagacggg cggggtggat ttaattctgg
    12301 ttgacagatc attccatcta tgttttttta gcggtctggg ggagagaagt cagtcggggg
    12361 ggaagggatg tgatggacca ggccggcggc ggggcgggtg aaaccgccgg gccgatctcg
    12421 ggcgtttcgc ccggccgacc ggcgcatcgg cggggcgtgg cgtggcggcg gccggcgctg
    12481 gtcgtcgcgg tgctcggcgc cggcctgctg ctggccgtcg tcgccgtcgg cgccgctctc
    12541 gaagattccc atgccaggat gttgttggcc gtgctcgcac cgccggcggc gatcgttgcc
    12601 gcgggcggcg cggcggcgct gcgccgctcg gtgcggcggt ggcgggaagt gaccgccgaa
    12661 ttgcgcggta tgcgggagcg gctccaactt ttcgatggga tactgagttc ggtccagcgt
    12721 tcgatcttca ttctctacaa tcgccagggg gacattgtcg acgcctgggg tccggcggat
    12781 ctcgccacgt ggttcggcct gtcgctcgac caggtgcgcg gccgcaacat gcgggagttt
    12841 cttctggccg agcacgcgcg gcgccgcgag cagatgctgg cgtgcgtgtt cgaggagggg
    12901 ctgccgcaga gcgggccgtg cgaggtgcag tttcagggca agctgttcag ttacgaggtc
    12961 tcaatttcgc cgctgcgcga cgcggcgggc cgcgtgacgg cggcggcggc gttctgccgg
    13021 gaaacgaacg agctgcgcgt ggcggagagc cggctgtatg aaagccgcga ggcgttgcgg
    13081 gcgctgctgg atgcggtaga tgaatcggcg tttctgatcg acgaggacta ccggttgctg
    13141 gcgctcaatg agacggccgg gcggcgcctg gagcaggcgc ccggggaatt gatcggccgc
    13201 gacgtgcgcc cgttgtggcc cgccgacacg gcggcgcggc gggcggccca gatcgagtgg
    13261 gtgcggcgga ccggggaggc tgtcgaattc accgaccggc gcaacggtta cgtgtttgag
    13321 aacaagctga tgccgatccg ggacgaacgg ggtcgagtgg tcaagatcgc gatcttcgcc
    13381 cgcgacgtgt cggaacggca acgggcggag gaggaactgc ggcgcagcga ggagcgctat
    13441 cgcgggatcg tcgaatcgca cgtggatctg gtcgtgcggc tcgatctgga ggagcggctg
    13501 gcgttcgtca acgacgcgtt ctgccggaag ttcggctacc ggcgggagga attgatcggc
    13561 cggcgatttt tgccgcagcc gcatcccgac gatgtgcggc tgacgctgga ggtactgcgc
    13621 gacctgcgtt ttccgccata ccggacgcac caccaggcgc ggatgatgac gccggagggt
    13681 tggcgctgga tcgcctggga gaacacgacg atctttgacc gggcgggccg gatcgaggag
    13741 ttccagggga tcggccgcga cgtgaccgac tacgtcaatt cgctggacgc gctgcggcag
    13801 agcgaggaac gttacaagag catcgtcgag aatacgcggg acctgatcat cgtgagcgaa
    13861 ccgggcgggg cgatcagcta tgcgagtccg gcatgcgagc aggtcctcgg ctggtcgtcg
    13921 gcggcgctga tcgcgggcga ggtgacggga atcccgcccg ctcccggcaa gccgtgcttc
    13981 gtccatccgg aggaccgcga gcgggtcgtg gcgtttttga gcgggcaggc gggcgatccc
    14041 ggggcgcgga cgctggagta tcggatcgtc acgcgcgacg tcgacgtgcg ctgggtgtcg
    14101 cacgcgtggt cgccggtgtg ctgcgggggg aagccgcgcc aggtcattca catcgtgcgc
    14161 gacgtgacgc cgatgcggat ggccgagcag gccgaacggc ggcggagtca ggaccttgcg
    14221 cgggtgtacg agaacgtccc gatcgggctg tggaaactct cgatggatcg gacgcggatc
    14281 ctggcgttca gtccgctgat gacggagatc acggggctgg acatgaacgt gatgttcgcc
    14341 gacccgacgg actgggtcga gcggttcggg gataatcccg aatcacggcg ggcgatgcag
    14401 atcatggacg agcggatcgc ccagcaactg ccgtttgacg tggaatatga attccggacc
    14461 gagtcgcgcg gcccacgctg gctgcaatcg atcgccaagc cggcgaccga gcacggcgag
    14521 gtggtctact acggcgtgac gctcgacatc acggagcgtc gcgcgatgga gcgcgagcgc
    14581 aacctgctgg ccgaggcgct caaccaggcc gacgagatga tcatcgtgac cgacgccgcg
    14641 gggcggatcg tctatgtcaa tccggcgttc gagcggatca cgggataccg gcgcgaggag
    14701 gcgctggggc agacgccgcg gctgatcaag agcggccacc atcacccgtc gcattaccag
    14761 ggactgtggg ggacgatccg ggagggtcgg gtctggcgcg ggcacttcat caaccggcgg
    14821 cgtaacggcg agatgttcga gcagttcgcg acgatcacgc cggtgttgaa cgaccagcgg
    14881 caggtgacgc atttcatcgg cgtgcagcgc gacatatcga tcgagcggga gctggagcgg
    14941 cggctgcgcc atgcgcagcg gatctcggac atcggtcaga cgatcatggg ggtctcgcat
    15001 cacctgaaaa acattctgag cggactgcgg ggcagctcga tgctgatgga gcggggcctt
    15061 gagacggggg acctgtgccg ggtgcgcgag ctgtggccgg tgttccagcg caacaccgag
    15121 cggatgtcgc ggctggccga cgagatgctc aactacgccc ggctgcaacg gctggagctg
    15181 gcgccggtgg atccgggtga actgagcggc gaggtgctgg agaaccagcg gtcgctggcc
    15241 gagcagcgac gggtgagcct ggaggcggag ctggccgagc cgctgccgcg gctgctgtgc
    15301 gaccgcaacc ggatgtacga cgcgctgctg aatctcgtct gcaatgcggt ggatgcgtgc
    15361 gacgacgtcg gcggcgggcg ggtcgggatt caagtccggt atctggagca ggagcggcag
    15421 attgagatct gcgtgcatga caacggacgg gggatcgacg aggagacact ggcgcggatc
    15481 ttcaatccgt tcttcacgac gaaggggagc aaggggacgg ggctggggct ggcggtggtg
    15541 cagaagacgg tgcacgagca cggcgggcgg atcgaggtga cgtcgcggcc ggggcggacg
    15601 tgtttcacga tgtggatccc ggttgacggc cggccggggg ggaaagagga gtaagcgcgc
    15661 gaaggcggag gacgcggggc gagggacgag agacgaatat gggcgggccg ttgggggcga
    15721 ttacgaggac gaggacgagg gaggaaatct ccgggggggt ggtcagacgt cgctgatgca
    15781 gcggtcgtag aattcggcgc agtggtcgcg gcggtcgccg gcgcgccagt tgatcgcggc
    15841 ccgcgggtgc tggttgagct ggccggtgat gatataggcc ggcagcggcc cccactcgat
    15901 ccggttgcgc agcgggatca tctgttcctc gatcaactgc cagacggggc ggattttgaa
    15961 tttggggttg cgcaggaagc cgaggagcaa ttccatcggg cagttgccgg cgccgcggcc
    16021 gaggccggcc atcgtggcgt cgatgcgatt ggcgccctgg atgatggcct cgatggtgtt
    16081 ggcgaagccg agctggaggt tgttgtgggc gtggatgccg acttccttgc cggcgggctt
    16141 gacggcggcg aggaatttgc gcaccagcag ttggacctgt tcgctgtgga gggcgccgaa
    16201 gctgtcgacg acgaccacga catcgacggg ggtctgggcg aggacgtcga gggcctgatc
    16261 cagttcggtc tcctggacct gcgagatggc catgatgttg caggtggtgg tgtagccctt
    16321 gtcgtgggcg tccttgatca tttcgacggc ttcggggatc tggttgatat agcaggcgac
    16381 gcggatgata tcgaggacgc tctgatcctt ggggaggatg tcggttttcc agtcggtctt
    16441 gccgccggcg tcggccatgg cggagaggcg cagcgcggag gggttgtcgc cgacgacgcg
    16501 gcgcaggtcg tcctcgcggc agaatttcca ggcgccgaag gcatcgcggg cgaagaggcg
    16561 gtcggaggcc ttgtagccga tctccatggc gtcgatgccg gcggcgacgc aggtgtcgta
    16621 gacggcgcgg acgaagccgt cctcgaagcg gtggtcgttg atcaggccgc cgtcgcggac
    16681 ggtgcaatcc atgacccgga tttccggacg ataggttacc cagggggctt tcattggatc
    16741 ctcccgaagc gcgtcggcgg ctcgcgtgga gcgcgtgaag cgcgtggagc cggaaaaggg
    16801 catgatccgg agggggcggc gggaaagtca aatggtattc gagggggaat gcgggagccg
    16861 agggattgga ggtcggacta ttcgagggcg gcagggcggg cggcggcgag aatgagcgac
    16921 tgggtgcgtt cgaccatggc ctggagggag aaccgggcgg cggccagctc ggcggcgcgc
    16981 cggcccatct cgcgccggag gggctcgtcg gcgacgagcc gctcgagagc ggcggcgaac
    17041 gcggcgggat cgttgggcgg gacgagcagg ccggtggcgc cgtcgtggac cgcctcggga
    17101 ttgccgccga cgcggcaggc gacgacgggg cggccgagcg ccatggcctg aatgattgca
    17161 ttggagaacc cttcggtttc cgaagcaagc gcgacgatgt cggcggcgtt gagttcgcgc
    17221 gcgacgcggg ggctgcggcc caccatcagg accgcgcgct cgaggccgag cgcgcggcgg
    17281 cgggcggcga tctcgccggc cagcgggccg tcgccggcga ggagcaggcg aacgggcggg
    17341 ccgcccgcgc ggcggaggcg ggcgaacgcc tcgagaagca gatcgtggcg cttgaccggg
    17401 ttgaaggtgg cggcgcagcc gatgacgagc tccccgggcg cgatgccgag gcgggcgcgt
    17461 tcggcggcgg cgtcgccttt ctcgcggtgc agccggtcca gatcgatgcc gttatggatg
    17521 acggagattt tgccgggggg gacggcttca tcgcgggcga cggcctcggc gaccgccccc
    17581 gagacggcga tgtagcggtg gatccgcggc tgcatccagc ggccgatgcg gatctggcgc
    17641 gaagtgcggg tgaaccccat gtcgcgccgg ctggagatga ccgggagggg gccgggccac
    17701 gcggcgagga cggcgaggaa atcggcgacg aaatggaagg cgacgaccgc gccgatcgcg
    17761 tggcggcgga gatagcggcg gacgcgcagg gcggcgcgca ggccggacca gtcgtaggcg
    17821 cgggtgagcg ggatgcgcgt gacgggcagc cagtcgaggg cttcgagctg gcggctgacg
    17881 atgccgggct cgcagtagat gacgtgcggg gcgatcaggt cgcgatcgag ccgggtgacg
    17941 aggtcgtgga aatgatgctc cgttcccccg gaatcgaggt gctgcaccat gaacaggacg
    18001 ggcaggggcg agggaggcat caggcgttcc gattggatgg gaattgcggg tcaatcatcg
    18061 agcgaaatcc attcgatggc gcgcgcgtcg aggaagacga gggcggcctg gacgcgctgg
    18121 agccgccaga cggcgcgcag ggcgcggccg tagcactcga tctggaggcg gtaatgttcg
    18181 cggagctcgg cgacgcggcg gccgccgtcg gtccaatcgg tcttgtaatc gaggacggtg
    18241 gccgcgtcgc cgtcgtcgag gatgacgtcg atgatgccct gaagcaggac ggtctcgcgc
    18301 aggccggcgc gggcggccgc gggggagaat tcggtcgcgt cgcgggcggc ggtgaagggg
    18361 agttcgcggc ggacggagag gggtcgttcg cgcattcggc ggcccggctc ggttgagaag
    18421 aaccagccga gggcgtcgta gtcgagaccg tccagttcat cggcgccggc cgcgagctcc
    18481 agccggccgc gggcggcgag gtcgcgcgcc tggcggcgga ttccgtcggg cgaaagatcg
    18541 cccgtcagat ccaactgggc gagcagggcg tgggtcagtg tgccgcggac ggcgccggtg
    18601 ggccggtggg tcgggggatc gcaccaggcg gggcgccggg cggcgagggc gccggcgcgg
    18661 gcgaaggcgg ggcgctgcat ttcctcctcg atgaaacggc tggcctcgtt ccagtcgatt
    18721 tcgccggcgg cggcgaggcg gtcgacgcgg tccagttcgg cggcgcgctc ggcttcgtcg
    18781 cgcagccggc gcagttgcgt cacggtcgcc ttcgcgggga cgcgggtcag cggggcgagc
    18841 gggtcgtgcg gggggacgaa cgcgggcgat agcggggtgc cgttgtcggc ggccggggcg
    18901 cgggcgagtt cgtcgagacg ctgcgcagcc gtgcggcagg cggcggcgcg cgcggcgggc
    18961 gggccggggt gcgccagcgc ctcgcggacg aagagcgcgt cgtccttttc ggcgcggcgg
    19021 gggagttcat cctccagccg gacggtcagc caggggggcg cgccgccgcc gtcgggggcg
    19081 cgcagccatt cgatgatcgg gccgagcagg tcggcggggg agcgggccgt cagccggtcc
    19141 aggacgccga gcgggcggcc gccggtctgg cgcagggcgc cccattgttc gggcagggat
    19201 tgagggtcgg gggcctggcc gaccaggacg aggcgctcgc gggcgcgggt catcgcgaca
    19261 tagagcaggc gcagttcctc ggagcgttcc tggcgcaggg cggcctcttc catgacgcgc
    19321 agggcgaggg tgccgtagcg ccggcggccg gccaggtcga cgcccctgag cgccaggccg
    19381 tggaggcggt cgcagtgcag ctcacggggg cgggcggttc tgaagccacg ttcgagccgg
    19441 ggcaggaaga cgatcgggaa ctccaggccc ttgctcttgt ggatggtgag caggctgacg
    19501 gcgcggtgct gctcgaccat ggattcgggg agctcgccga gttcgaggct ttcggattcg
    19561 gcccgttcga gcagctcgag gaaagcggcg aggggattgg cgccgtcggc ggcgtggccg
    19621 agccgggcga ggaggtcgcg caggtaatcg acgcgctgga ggcgctcggc ggcgtcgggc
    19681 ttgccgcgca gccaggcttc gtagcgggta tcgcgcagga tggccgcgag gagttcgacg
    19741 ggggtgttgg accaggcgag gcggcgccag gcggcgaggg tgtcgccgaa ggcgcgcagc
    19801 cggcggcgga ggtcggcggg gagatccgcc gcgtcggggt cggcgcacag gtccatgacg
    19861 gcggcggaaa agggctgttt gcgccgggcg aggcgcagcc gcagcaggtc gtcgtcgctg
    19921 aaggcgaaca tcggcgagcg catcagcgcc gccagcggga catcctgcag cgggttgtcg
    19981 atggcgcgca gggcgtggac caggtcgatc agctcggggg cggtgaagag ggggctgagg
    20041 ccgggggcgc ggacggcgag gccctcgcgt tccagcgcgt cgctccagac atcgatctcg
    20101 ttcatcaggc tgcgcaggag gatggcgcag tcgcccggga cggcggggcg ccaggcgccg
    20161 tcgccggtgg ggatgcggag cgcgccggcc gggtcggtca gttcgcggat gcggcgcgcg
    20221 acccaggccg cctgggcctc gacgccggtc agctcctcct cggcgcggac ggaggaatcc
    20281 tcctcgccgg cggcctcggg ggccgggggc tcggcggcgc ggggaagcca gtggacctca
    20341 acggccggct cgccgctttc cagttcgtcg gggcggccat ggacgagccg ttcgcggtcg
    20401 gcgtagcgca gttcgccgac ctgttcgtcc atcaggcgct cgaagcagtg gttgacgaat
    20461 tcgatcaggg ggcggcgggc gcggaagtta tcgggcaggt cgatgcgcac gacgcggcgg
    20521 atgccgggga cggcctcgcg ctcggcggcg cgcagcttgt cgaggaacag gccgggttcg
    20581 gcatggcgga agcgatagat gctctgcttg tagtcgccga cgatgaacag gttgccctcg
    20641 gcgttgggcg agggatcgtc gaggcgggtg acgcggcgga tgagggcgtc ctgcaggggg
    20701 gaggtgtcct ggtattcatc gacgaggacg tagcggaact gctcctgata ggcgcgggcg
    20761 atgtcggagg gggcgtcggt cgcggggtcg cagaggacgt cgaaggcgat ccgttcaaag
    20821 tcgttgaagg tggccaggcc gcgtcggcgg agggccgcgt cgatggcgcg gcgggcctcg
    20881 tcggcaagtt ggagcagggc gcgggcctga cgcgcggcca gccgggcgcc gtggagcagg
    20941 ccggcctcgt cgagcgcgaa ccacttgtcg cgccaggcgg cataacgccg ggcgaagcgc
    21001 tcgagggtat gcttgcggaa cgcttcgttc tgataaagag cgtcgccgca gttggttttg
    21061 ttttgcaatt tgtccagcgt gccgtcggca aattgggcga gccagtcgcg cgcggcatcg
    21121 agctggaggg gttcgcggcg ggcgagcgcg tcgagaccgt gggcgatcca gcgccaggcg
    21181 tcgaggcagg cggggtcgcc gccgcgggcg ccggcgaggt cgatcaggtc gcaaagcgcg
    21241 cgggtgaggg cggcggtgga atcggcgaag gccgcgcgcg ccgcggcggc ctcgggcagg
    21301 tcggcgaagg tctgggcgcg ttcgacggcg tcgatgcgtt cgcgcgcgcg gcggagccac
    21361 aggtcggggc gatcgagcga ttggaggaag gccagcagtt cgcggagttc ggccgcgagg
    21421 gcgcgcgctc cgccgggctg acaagccagc agccattcga gctccgcgcg cggcgggccg
    21481 ggtgcggcgg cgcgttcctc gagcagttcg tcaaggacgg ccaattcgag ctgggcgcgc
    21541 tcggcttcgt cggggacgcg gggattgggc gcgaggccgg cgcgatggaa gtgatcgacg
    21601 acgacgcgcc ggcagaaggc atcgatcgtc atgatccagg cgccgccgag ccggtccagc
    21661 gcgtcgagca ggcgggggtt gtcgggcgcc gcggcaagac cctggtggat gcgccgctcg
    21721 atgcgttcgc gcatctggcg ggccgccttc tcggtgaacg tgatgatcag catctggtcg
    21781 atgcgggcgg ggggctcgcc ggcggggccc ggttcggtga tgaggcgcag gacgcgttcg
    21841 gtcaggacgg tggtcttgcc ggcgccggcc gaagcggaga cgacgaggtt gccggcgcgc
    21901 gactcgatgg cggcgcgctg ggcgggggtc cagcgggggg ccggcgtagc gggagcggcg
    21961 gcctcagtca tcaccggcct cgcattcggg ttcgacgttg aaccggtcga tgatgcgcgc
    22021 gcggtccatg ggcgggatcg tcacgggttg gacggcgccg ggctcgaagc gccggcagag
    22081 agggtggaag gcgcagtggc ggcaggcggt ttcggaccgg tggcggaagg gggtggcggc
    22141 gatttcaccg cgggcgatgc gggcgagcag gtcgcgcagg aggcgccgct cgtcgcgcag
    22201 caggcggtcg aacgcgatgg cgggccagtg agcctggccg cggccatgca actggccctt
    22261 gctggcgcgg cggaagccga aggcgaccga ggcttcgccg gggccgacgg agcggtcgag
    22321 ggtcgcggcg aactcctcgt tgaagatgcc gcgcggggcc agccgttcaa accagcgagg
    22381 atcgaccgga tcgcactggg ccgcgtcgcc ggcctgggcg tcgtgaagcg gcgccgccag
    22441 cggccagtag aagatgccgg cgatccggcc ggggatgccc cggcggtgca tcgcctgctg
    22501 gaaggcgagg ccgtaaacgg ccaactggag gtcgaggccg gccatcaggc ggctgatgtc
    22561 gaggacgtgg cggccgctct tgtagtcaaa gatgagccag gcgaagcggc cgtcggcgtc
    22621 ggcgagctga tcgatgcggt cgaccttgcc gacgatcgag aacgaggcgc cgtccgcgag
    22681 ccggacgtcc agttcgcccg cctcgccgaa ctgccattcg gcgtgcgcct ggcggaaggc
    22741 gcagtgggcg ccggcgaggc ggagggcgcg gatgaacagc ttgagctcgg cggccagttg
    22801 cgcgagggtc aggtcgttca gggcgcgggc ggcgaggcgc cggctccagc ggggattgga
    22861 gcggagttgc tccaggcgac gctcgatcgc ggcgtcggcc gcggccggat cgatctcgcc
    22921 ccaggcgagc gtgggcgccg tgccgcgcag atcatggaag acgcgctgaa ggaggtcgtg
    22981 gcggatggag ccgagatcga ccggctggag ctcggccgtc tcgcggtcct ggaggcgcag
    23041 gacctgctcg gcgaaaaacg agaaggggca ggcggcgaag cgctcgagcg cggtgacgcg
    23101 cacgcgggcg cccggttccc agacggcggc ggccaggtcg ggatcgagcg cggggcgggc
    23161 ggggggcgcc agcgcggaga agccgacgcg gagcgcctcg cgcgtgggag gggccaggtc
    23221 gtcgcggtcg aggggatgcc cggcgcggag cgcggccgcc agttgcgcgc gggcggggcc
    23281 caggtccaga tcggccagca ggccgcacag gcggctcatc cattcctcgg cccgcagggg
    23341 gaggcccggc cgttcgtcgc gcgcgtcgcg gtcgacgagc gaggcgggag cctggggcgc
    23401 cgccgcgcgc accatgcgca ggaaagggga gggatcgccc gccttgccgg cgtcgtcgag
    23461 aagcggccgg gtcaggacga gggcgtgcga ggcgcgggtg agcgcgatgt agccgaggta
    23521 atgctcctgg ctgcggcggg cctggaggtc ggggccaatg gcgccgccgt cgcgggtcag
    23581 caggtcgcgc tcggcatcgc ccagaatcgg gtcggggttc caggcgcggg ggaattcctg
    23641 ctcgttgagg ccgatgagga aggcaagctt caactcgggg gtgcgcgagc ggtcgacggc
    23701 gccgacggtg acctgattga gcgtgggggg gatgaggcgg gcgcgggtgg cgcgcagcgc
    23761 ggtatcggcc agcagcgcca gatcatcgag ccgggcgtag taggcctgca tcgggtcggc
    23821 gtcggcgggg gcgggggcgg gcgggagccg gtcgagttcc tcgagccatt cgatgacctg
    23881 cgcccaggcc tgttcgtgga ggagcgcgga ttcctcgtcg ccctggcggc ggtcgcgctc
    23941 gatccaggcc tcgagcgtcg cggcggcgcg gacgtcgtcg agaaggcgcc agagcgcgcg
    24001 ggcgggcgga tcgccggcgc gcagggtttc ctccaggcgg cgcagcgggg cggcggcccg
    24061 gcgccagcgg tcggcgatct cgcgcatcca gggcgggccg tcggcggcga tcttgagcca
    24121 gcggcggccg tcggggtcga gcgcgaggat caggttctcc agttccgcga cgtgaagcgg
    24181 gtcgtccagg ccgctgaggc cggttttcag atagtgaacg acctcgcggc cgcggccgcg
    24241 aaagcgtagg aggcgcagcg cggccaggat cagccgacag aggggatggc cctcgatctg
    24301 gcgcgggcga tccaggaaga acgggatctc gaactggcgg aagacctcgg ggatgagatg
    24361 ctgatagggg gtcaggtcgc gcgcgatgag gccgatatcg cgccaggtga ggggcctcgg
    24421 tccgtgggcg gcggggcgat cgacgcggca gagggcgacg atccggcggg cgatcgcctc
    24481 gatttcggcg cgccggtcgc gcgcttcgat gatttcgatc ggcgcggggg tccaggcgtc
    24541 ggcgggcgcg gcgggcagca gctcggccgc ggcgcgggcg ctgcgttgcc agtcggcgtc
    24601 gtccagcgcg cgggggagga gttgggccgg caccgtgcgt tcgatgtgcg ccagggcggg
    24661 gctggcggcg aagcgggtcg gctggtccgg ctcggggagc gcgatgattt cgacatgcca
    24721 atccagttcg cggaagcgct cggccgcctg gcgggcggtc tggagcgtcg agtggaacag
    24781 gcgctggggc ccgacggggt gcgggcggtc gccgccggtc ccgaggaatt cgattgggtc
    24841 gcacgccagc gcgaggacca gcgaggcggc ctggcgcgtg acggcctcga ccagccgcca
    24901 ttcgagagtg gtgaacgagg aaaagccatc gatccacaga tgggcgccgt gcaggtcggg
    24961 ccattgggcg atggcctggg ccgccgccgg gtagaaggcg agcatgtcct ccaggccgag
    25021 ctcggcgagg aggcgctcgt aatcgcgcca gatgagagcc agatcggcca gtttgcgggc
    25081 ggaggggtcg gtctggcgcg ccgcgggggg catggccgcc agccgcgcct ccagatcgtc
    25141 gggccgccag ccgtaacgct ggagttcgag gagggcctcg tggaccgatt cggcgacgcc
    25201 cggctggccg gccgtggttt ggaagacaag cagccgggga cgcagcgatt cgagcgcgcg
    25261 gcgcaggagc atcaggcgac cggcttcgcc aacgcgccgg gccggggggg cgccgcgccg
    25321 ggcgagcgcc tcgcgtgcca gatgattgaa cgagaagacg cgcgcgcggg tgaagccatt
    25381 ctccacgccg ggcatccgca ggaggcggcg ctccatctgg gcggtggcct gttcgggcac
    25441 gagcaggccg agcaggggtc cgcgcggcgc ggcgcgcagt tcgtcggcga tggcgctcag
    25501 gcaaccccag gttttgcccg cgccggcgcg gccgagccag atctcacagg ttttgacggt
    25561 ggaccagcgg cgtttcaatc cggcggtttc cttggaaaag acggcattga tgagccagct
    25621 tacgccgacg ccgggggggt ccgcaatctt caaccgcccc ggccgggggc cgcgcgcggc
    25681 cgcggcccgt cgatctcgca accggagtga atgcaatctt ccgcatgcgg tcaaggcggc
    25741 ttttgctggc gtttcggggg gagtcttgct agattgcccc ccggctcgcg agagagttct
    25801 cccgatgggc tcgggggtgg atgccccggc tgacagcggc gagaaggggc gggttttttt
    25861 tggtgaggcg atttgttgag gagtgaggag aggtgagttg gacgcttaag cactaccgcg
    25921 gacagcgtgt ccagccgacc gggatggggt tgctgggtct ggtcggattg agcctgagcc
    25981 tgggctgcgc gacggcgccg ccgccggcgc gggtcgccac ggtgacgacg gcgcggcgac
    26041 cgccggcctc gccggtgcgg gtgttcatcc cgaaggtgat ggtctcgccg gaggaaaacg
    26101 gcaagccggt gtatgagaaa gtcgaggatt acggcgacaa gtatccggag acgacggcgc
    26161 cgccggagca ggggacgatc gaaatgctgt cgcatgacga tccgctgctg agcgagttcg
    26221 aggagttgcg gatccgggga gaggcctggc cgatggtcgc caaccggggg gcgtcgcagg
    26281 tttacccgcc cgaggaaggg gtcgtctcgt tttactggca ggcgcagcgg ctggcgtcgg
    26341 gcgattactt tgatccgggg aaactgtgcg ccgcgcacaa gacgcttccg ttcgggacga
    26401 ttgtgcgctg cacgcggctg gacaacggcc agtatgtcga cgtgatcatc aacgaccggg
    26461 ggccgtatat caaggggcgg gtgctcgact tgagcaaggc ggcggcgagc cggatcgggc
    26521 tgatcggacg gggaatcgcg cgctgccgga tcgaggtgct ggcgtatccg ttgatcgaga
    26581 cgatgggccc caacgggaac ggctgagtct gattttcaat taaagccaat cggaaagacg
    26641 accggctcga ccgccggcat cagttcggcg ccgacgacgt ggacgccgta ggtatcgagc
    26701 agcgtgccgc gggcgcgttc gagccggatg gcggcccggt tgtagtcgac gacggcgcgc
    26761 agatgattgg cctgggcctc ggcgaggtcc tcctggaact ggaggacctg gaacgcggtg
    26821 gagacgccga catcgaggcg tttgagttcg gcgtcgagca gcgcctgggc ggcgcgcacc
    26881 tggctctggg tcacgtcgat ccgctcgcgg gcggtgcgca gcaggcggac ggcattgcga
    26941 acgtcgaggg cgacccggtc gacgaccgcc tgcagatttt cgccggccat ttcggcctgg
    27001 gcgcgcgcct ggcggtggcg atagcgcgcg gcgcggttct gcagcgggta gctgaactga
    27061 agcgcggccg tgtagctgat gtatttgccg tccttggcgg tgcgataggc gcccgtgccg
    27121 tcgtcgtcga ggccgctgga ctccacttcg ccgatcagat cgaggcgggg gaggaggttg
    27181 ttgcgggcga cctggagctg gacatcggcc tgatcgatgt tgctggtggc gcggcggatc
    27241 tcggcgcggc ggtcgagggc gacgtcgatc gactcctcca gatcgacgtc gatttcgcgc
    27301 caggcgaacg gctgcgaggg ccggagctgg gcctcccaga gcggggcgtc gggctggagc
    27361 agcaggatct gcttgagccg gtcctccagc tcgcggacgg accggcgggc gttgatcacg
    27421 tcgacgcggc ggccgccgac ggccgcttcg gcctggagca catcggtcag gggaaggacg
    27481 ccggcatcgt agcgcgcgcg gttgacgcgc agcaggtcgg cggcggcgct gtaggaaacg
    27541 acggcgacct tgtgaagctc gatcgcctca atcagttgcc agtaggtttc cagcgccagc
    27601 agcagggtct cctcgacctg ggtctggaag tcggcggcgc tcccttggcg ctccagttgg
    27661 gcgatgcgga tgttggcgtt ggtgacagcg gggccgaagc cgcgcaggag gggttgggtc
    27721 agcgcggcgg tgaagctctg gcgatactgg acgtcggtgc gcgtggtgga gctgaacatc
    27781 ggcaccggca ggccgagggg gttatccggc gagacgatgc ccgggaggaa cgccaggctg
    27841 atgctggaga acaggttggt gctgcgggcg atggcatagc ccaggtcgag cgtggcgccg
    27901 gtggggaaga gctggctgag gccggtgaga aactggcggc ggcgggtgct gttgatgccg
    27961 gtcgaggcga agctggaggt gctggtctgc tcctcgatgc ggctgaattc ggcgcgggcg
    28021 ttgagcatca ggtcgtagat gcccttctgg accgggattt cgtattcgtc gatccgcagc
    28081 gaatagccct gaatctggat ttcgtggttg ttggccagcg tttcgatgac gacgtcggcg
    28141 acggagagat ccagggggga gctgtcgaac aactgggtga tggaggccgg caggtcgtcg
    28201 tagccgacgg ccgtctcggg ggtgatccgc acgtcgtcga tgacgacgtg ggaggcgcgg
    28261 tcggaggggg cggccgcgac gggcgccggc ggggtttgca gcaggcgctg ggtatcgacc
    28321 tcgtaggtcg ggagttcgat gggcggggtg gcgcacgccg ccaggatgac caggggcgcc
    28381 gccaggagta gaaacgacaa gggacgcatg ggcgaggacc cggcgggcct ccttcacggc
    28441 aagatccccc atagcctacg cggggcgggc gaaataatca atttgcggtt cggggggaga
    28501 tcggaaaagc ccgagtgtgg ccggtttcga gagacgaggg cgggggacga gagccgggag
    28561 ccgaattctg gggtgggcca ttggggttct gtggagtgcg tcggcagaga ccgcgcagcg
    28621 gtcgtcgacg ccgctttgga tcggtcggaa tcagct
//
LOCUS       DAOPCO010000021        28596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1796_length_28596_cov_5.965839, whole genome shotgun sequence.
ACCESSION   DAOPCO010000021 DAOPCO010000000
VERSION     DAOPCO010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28596
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1796_length_28596_cov_5.965839"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..921
                     /locus_tag="PLS90_03070"
     CDS             331..921
                     /locus_tag="PLS90_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK01418.1"
                     /translation="MNSISSAREQADQLRELLERIAQQPDLLDTLDRICRTVVEALAA
                     GGKVLTLGNGGSATDALHLAEELIGRYRTDRAPLPAVCLSADVSALTCIANDFGYDAV
                     FERQVQALGRPGDVVIGFSTSGNSPNVNRAFGAARAAGATTIGMLGKTGGAARDLVDL
                     AWVVPHDNTARIQELHTLALHAICEAVEARFGLSST"
     gene            986..2014
                     /locus_tag="PLS90_03075"
     CDS             986..2014
                     /locus_tag="PLS90_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK01419.1"
                     /translation="MNLTPREIVLRTLAFDHPPRAPRQLWVLPWAELHHPGAFDRIQA
                     EFPPDIVYADGHQREPRQVQGSPYERGVYIDEWGCRFTNIQPGVHGEVCDPLIRDWDD
                     AGAIRFPREMLTIDPDAVNRDCEASDRFQLVPYCPRPFERLQFLRGSQALYLDLMMRP
                     PAMLAFLRKLHGFYCEFLELWCSRTQVDAIMFMDDWGSQRNLLIAPELWCELFKPLYR
                     DYIEIAHAHGKKAFMHSDGYIVAIYPHLIELGLDALNSQIFCMGVENLRPFAGKITFW
                     GEIDRQQLLPHGSLEQIDAAVESVYHTLWRGGGCIAQCEFGPGGKPENVRRVFEAWDR
                     LTSPAMTK"
     gene            2111..4045
                     /locus_tag="PLS90_03080"
     CDS             2111..4045
                     /locus_tag="PLS90_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPK01420.1"
                     /translation="MTLQQITLHDAALPRHDLEALMHDIQSVSVAVLGDFCLDVYWFT
                     DLSAAELSLETGLETRPVRRAHLSLGAAGNVAANLLALGCRRIEAFGVVGCDIWGREM
                     LHLLRESGVGTRGMLSEPRDWSTLVYVKPHIQDKETSRFDFGNFNCLPAQTARTLIGA
                     LVERLDEFDAVLINEQVTNGLHSAELRQALAEAIQRHPGTNWIVDSRHFPEAYPGASL
                     KINDLEATRLLGIERPAGALVPREEALTAAEALEQRMGRPVYVTRRDRGVIVHDGRAP
                     QEIPGLLILGRIDPVGAGDSMLAGIAAAIAAGRSPVQAATLGNFVAGVTIQKLYVTGT
                     ASPDEVLRLGAEPDYVYRPELAEDPRGAVYDDGAEIEIVSRRSPAGRPTVAIFDHDGT
                     ISTLRQGWEAVMEPMMIRAILGEQYQSADESLYHQVVDRVREYIDHSTGIQTLLQMQG
                     LVRMVREFSCVPEDQILDEFGYKAIYNEALMAMVSRRLAKLERGELQVEDFLIKNAWA
                     LLERLRDAGVRLCLASGTDQEDVRREARALGYADLFDGGIWGSVGDVNKDAKRIVLDR
                     IMNEIGDVGMAGLVTFGDGPVEMRETRRRGGFAIGVASDEIRRYGLNLEKRSRLIRSG
                     ADLIVPDFSQLDRLLDCLNL"
     gene            4191..5312
                     /locus_tag="PLS90_03085"
     CDS             4191..5312
                     /locus_tag="PLS90_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007279487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01421.1"
                     /translation="MPYPQLDRSRVNMRPLRERKNKKRIERDHIALDTQPRGLTPSDE
                     RLIAEVAERIEASRAADRSVMLAFGAHTIKNGLAPVLIELIRRGWVTHLATNGAGVIH
                     DWEFAYLGESSEDVRANVARGEFGNWHETGFFINLAINVGAWEGRGYGEAVGALIQRE
                     GLEIPTPARLEQEVRAVLKADPARAAAAAELLGVLRRFELEPGWLAVPHPFKEFSVQA
                     AAFELGVPSTDHPMFGHDIIYNHPMNNGALLGRAAERDFLRFAAGVDRIAGGVYLSLG
                     SAVMSPMIFEKSLSMVRNLAMQEGRRIDDHYMLVVDLQPSHWDWRQGEPPEDNPDYYL
                     RYCKTFARMGGTMQYLSADNRDFLLALHRALSGRRAPGE"
     gene            complement(5343..6299)
                     /locus_tag="PLS90_03090"
     CDS             complement(5343..6299)
                     /locus_tag="PLS90_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPK01422.1"
                     /translation="MIRKVNVAAFTIDRFDKQPRTLEDNLNYASDVIDEISGSEQPDI
                     ICMTETFDTRLVSSCHPEMAQTADGPTVTRMREKARQHGCYIICPIIERRGEHIYNTC
                     VVLDRNGEICGRYDKIHPTAGPGCEVEDYIIPGDPAPTIIETDFARIGCQICFDANWW
                     RNWDELKQAGAEIIFFSSAYPAGRVLHSMATLFHVPIVAATCRTSCRIIDIDGLDLNW
                     EAIYQKWVAATLRLDSPLFHLDFHFEKIDRLRADLGRAIEIKIYPEEAWWRILPRDQE
                     VDVQELIRAYELVPLEQYLANAEAIQCAGRREMKKTPVLSQV"
     gene            6569..8479
                     /locus_tag="PLS90_03095"
     CDS             6569..8479
                     /locus_tag="PLS90_03095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPK01423.1"
                     /translation="MPRLCIPLVLTLVVALIAPAPLPAQSAGSDMPTEITERLWRDPG
                     PEQDGPGAPRLMVVYPAAAGGPYDGEAMRYAGRVDPEDATVTLNGDELQVYPGGVFTG
                     LRRFGDAESETWTFRAAAGGKTTTVSRSLRIRRPTPPPAHWPLQFASWPHSPSGLFVL
                     PEGEELEIKLFASPGHSAEYRIGEAGAWRAMTPAEPHPRLGGVYTASLTPPLLDDAGP
                     RTVHFRLSGSHEGRSDRIERLSSLQVAARPDGVRYAGRVTNYLANYLKHSDPENWDRY
                     GQLVKGTPFPVTEVRGDRCKADFGRGETGWLELEHVKIDWEPFAGPAPRLDETRVVVT
                     SDSLTLAWPDAAQPVLYVFDDRSHAGPEHDVRLSLPGADESMRCDPPDSLPGGPVDSL
                     QFRPAEGAAAPAFLISLTRPLWGYSMLAGASDEADQPGLRFVLRTRPRLPAATAAKPL
                     AGARIMLDAGHGGDDLGAIGPSGLVEADLNLVQAAWVQTYLEELGAEVEQTRRSHATV
                     DLDERALAAHRWDPDLFVSLHHNSVGFTSDPLADSGPIVFYHYDHARPVAETVGEALT
                     SAIAPDRSPRVNSAVFRVARNVLLAPSILIETGYVCNPHDEAALRRTESIKQSARAIA
                     EGIRAALTEPAD"
     gene            8586..8912
                     /locus_tag="PLS90_03100"
     CDS             8586..8912
                     /locus_tag="PLS90_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK01424.1"
                     /translation="MPASAYNCHYVHATGTLQIVLLPGFRQEYWDELMHYVENTDRSG
                     NYRWDLDLRRIEVINSMMLGLIVGLRARLDARGGNLRVLIAPNSKVEQLLQLTRLDQI
                     VETVKA"
     gene            complement(8966..10696)
                     /locus_tag="PLS90_03105"
     CDS             complement(8966..10696)
                     /locus_tag="PLS90_03105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPK01425.1"
                     /translation="MNQTLDRAQHSSDDAPCRLVVEEGEQKGMVFPLRDKVVSVGRGP
                     DNVIQIIDPRMSRNHTLFILNGSQWLVRDLGSKNGTLLNGRAIPGDQVLGHGDRIQIG
                     DTVFAFELEPTHDGHVETSSGVRVLDDHGMLVPSQILRMSEKSSGDSTAPLYTMPYAG
                     NRQLGVLYRLLDVTSIVLNLDELLEKIIDLVQETLKPDRSGILLHDERYGILLPRAVR
                     RPPDSTDDIIISNSIITKSVEDEVAIIVSDAPRDARFRASDSIVIQRIHSALCAPLIF
                     NNEVLGVIYLDRRRPSGSFGEEDLKLVAGIAKQSAVAIANACFHSQLLEQHARARELE
                     IARTIQEHLLPKSMPELDNFEISGTTRPAYMVGGDYFDVISLPDGRLVLAIADVTGKG
                     VPAAILLAAIRTAVQIEVRSLGNETLATIVDRINQMVCRDTSNSMFVTMVLGVLDPHT
                     RQFTYCNAGHVHPLLCLADGTLRTLESGGTLLGVMPGINYETETLELPPGGMLLMYSD
                     GVTDAQNVKGDMFGQQRIINYLRRHVGEPAADFRRGMLEEVNRFSRDAEAFDDVTILV
                     AKSLDRRDTF"
     gene            complement(10840..11841)
                     /locus_tag="PLS90_03110"
     CDS             complement(10840..11841)
                     /locus_tag="PLS90_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01426.1"
                     /translation="MSLLPHAPSLRRLASALGPLFGLGLIVVIFRIIVGEGFTDWYNV
                     QTILADTTIVAIAALGMTLVIIGGGIDLSTGSVVALASVVCAKSLAGLIGVDGAMTPA
                     AWCAAFGAGIGIGMLCGAINGLAIVGLRVVPFIATLGMLSIARGAAKWQSANIPINIT
                     YDFGEWVQPYLPEGWSWLLVAPSVWLALGLALVFGLVLHRTVYGRHVVALGSSEPTAR
                     LCGLRIGWLKISLYVVAGAMAGLAGVVQAARLTRGDPSVGVGLELNVIAAVVIGGGSL
                     MGGEGRILGTLAGALIMSVLATGATQAGWENYIQEILVGLIIVLAVALDQLRARRAA"
     gene            complement(11928..13454)
                     /locus_tag="PLS90_03115"
     CDS             complement(11928..13454)
                     /locus_tag="PLS90_03115"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPK01427.1"
                     /translation="MPESPLLHAHAIQKHFGATHALDGAELSIGRGEIHALVGENGAG
                     KSTMIKILAGALRPDGGRILWRGQPYQPARPADARRRGIATCHQELAIAPHLTVTENV
                     LLGQELARAGWIRRRPSAERVRALLALLEQSHIPLHLPAGRLPLAQQQFVEIARALAA
                     DAQLIVLDEPTSALTQADARHLFDRIRALRDQGVSFLYVSHFLEEVMELCDRYTVMRD
                     GRTVDSGRVAEIGPDDLVAKMVGRRVDQLFPRPARTPGAEVLAVANLSGPGGRPAGVS
                     LSLRAGEVVGIGGLVGSGRSELLRTIYGLLPPASGEAVLIAGDRPRPLTAGADRRIRL
                     GLGFASEDRKNEGLALRLSLAENIGLGALGRIARIGWLSGARLRRLAIPAADALSTVY
                     RDIGQRALDLSGGNQQKVALARLRFSQARVLLLDEPTRGIDVGSKVLIYEWLNREAAE
                     GKAILLTSSHNPELLGICDRIGVMYRGRLVALAPRAQWTEERLLRAATLGEPPPREEG
                     "
     gene            complement(13780..14793)
                     /locus_tag="PLS90_03120"
     CDS             complement(13780..14793)
                     /locus_tag="PLS90_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK01428.1"
                     /translation="MPVHFHSLARSVWRLAAVAALVAFAAPPLHAQAGEGSNIVVIPK
                     GTTHDFWQAVHAGAAKADRELDQVAITWQGPLREDDRNSQIQIVQSHVAKKTDAIVLA
                     PLDARSLRNAVIQANRRGIPVVIIDSDIEREGVEIVSFVATDNERGGELAGRELGRLL
                     GGKGHVIMLRYAVGSASTEAREAGFLKAIAEFPEIKVISENQYAGATSDEALQKSNSL
                     LNAMKDQQIDGIFCPNESSTFGMLLALRNHGLAGKVRFIGFDASPKLLDALKAGQIDG
                     LVVQNPFLMGYEGVMAAARALAGDEVAERIDTGVALVTRENFELPEIQEVIAPPLEEY
                     LKE"
     gene            15243..15404
                     /locus_tag="PLS90_03125"
     CDS             15243..15404
                     /locus_tag="PLS90_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HPK01429.1"
                     /translation="MSIVGMILLILLIMIVLGSLPAWPYSRGWGYYPSGSVGLILIIV
                     IVLLVLGVI"
     gene            15468..16727
                     /locus_tag="PLS90_03130"
     CDS             15468..16727
                     /locus_tag="PLS90_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK01430.1"
                     /translation="MEVFEHVILGGGVAAGHAAREFVKRGVKPGQLALVSAEASFCYE
                     RPPLSKALLAGTKRPEETLISPPRFYAENGIAFYHEWRAVGIDFGRRLMMNDQGETIG
                     FRKLMLATGSRVRNLAIAGADLLGVHYLRTLEDARAIVGALRDVRRMVVIGGGFIGAE
                     VAAQTARRGINTTLVAATDHLWPGHFTPEISAFFKGYFQSNGIHLIFSARPAALHGPE
                     HVTRVTLETGQSLPAEMVVVGIGVVPELELYRQTGLQLDAGVLANQFLETNIPDVWAV
                     GDICQYEDVLFGKRRRIEHWDNAKSQGEHAARAMLGRREPFIHVPYFFSDVFDLSWEF
                     WGDTEEADRVLYRGDLGRGGISAWWLRGDQVHAAFVMNRPDEERRAAPLLIAHGTPIN
                     AECIANMEIPPAQWTPAQNCETGPSLA"
     gene            16827..17000
                     /locus_tag="PLS90_03135"
     CDS             16827..17000
                     /locus_tag="PLS90_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HPK01431.1"
                     /translation="MLYWALVFFILALVAGLLGFGGLYAAFATVGKILFVIFLIVFLV
                     SLIMGLVGGRRVV"
     gene            17387..18451
                     /locus_tag="PLS90_03140"
     CDS             17387..18451
                     /locus_tag="PLS90_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01432.1"
                     /translation="MNSKITVTQVPLQQLIDVLDVGILVLGRDGQPDFSNAPARMHFG
                     CTRHDDLVERWQQIDRELNEIPTDGQGAVACTVQLEVPGPVRARRLQLEFYPLNHEDC
                     EGQIVLIRDREMLDAMETDLRLASRFRGLTRLYRAIAHDLKAPLNAMVINLELLKETI
                     NLPHRDASTQERKDRYVNILMSELGRLNRTLQLLLTQADLSTKSSERIDLGEILLDVV
                     TLLTPQAKQQEISVNYHLPEEPVLIIGSEDWLKQAVLNITINALEAMDRGGRLTIRLH
                     VKEHVATLLVEDTGSGISQEDLEKIWNMHFTTKSSGTGIGLYVSRSVIQSHGGSIDVM
                     SVPGEGSLFRITLPTAGKEN"
     gene            18460..19830
                     /locus_tag="PLS90_03145"
     CDS             18460..19830
                     /locus_tag="PLS90_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK01433.1"
                     /translation="MRALVVEDDASCQTALAELVQMKGFEVVTAGSIKEARSQLRKKA
                     PDLALVDLNLPDGSGMGLINEIDEKYPSEVVVITGNTNLETAVEAMQQGALDYLTKPI
                     DVTKLKKILDDLAQDTARRTDNSLQAKSSAILRASQMVFESPAMHEIVTLVTRVAATE
                     ASVMIIGESGTGKELVAQMIHDKSPRADGPFLPINCGAIAPNLIESELFGHERGSFTG
                     AATRHLGYFEQASGGTLFLDEVTEMPLELQVKLLRALETSQIMRVGSGNPVNVDVRVI
                     AATNRQPEGAIEDGKLRRDLYYRLNVFPIEMPPLRARPEDIVCLGRHFLAQFNAAYET
                     KKVLTDEGMEKLCSHSWPGNVRELRNLIYRAFILADDEIGPDVINFEKAANGHPGASV
                     DLNMKVGTSIADAERRLILATLDAYQGNKKKTARVLGVSLKTLYNRLNELKPEPAPAA
                     KPARSR"
     gene            20170..20361
                     /locus_tag="PLS90_03150"
     CDS             20170..20361
                     /locus_tag="PLS90_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01434.1"
                     /translation="MLTHFTVALRRVLMLLALLFASVAVMPTLVACNGGAEDNLEDAG
                     ENLGDAVEDLGDAAEDALD"
     gene            complement(20444..21475)
                     /locus_tag="PLS90_03155"
     CDS             complement(20444..21475)
                     /locus_tag="PLS90_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01435.1"
                     /translation="MKTAERLSQLVGLPRRVAAAGLLCFTLAAGAAPAELDWEALRNP
                     ILGDHDQSLHDFCVVEADGLFHIFCSAFQESGDAPNCTIVQYTSADLVRFAEVNRLDG
                     ASRGWRGTASPYVVHDGNRYVLVGNSWGDAPEQPNALFWCESPDLMAWSSPRPLAANL
                     TGEARAVDPALAPFGEVHLLIWNQGRLPRVAHGERLVGDFAWLGPGYLELSTVDGVHR
                     PYFASFQVLRIGEGWRLLAAAREDRHRPYLFERPESTSAGLRFSRWENGTALDIPVQD
                     FNREILANGAWLLDRRATDGWFYLFYAGSDGQRRADFGGRGWNRLGIARSRELARWEP
                     AGMAGAAVE"
     gene            21651..22610
                     /locus_tag="PLS90_03160"
     CDS             21651..22610
                     /locus_tag="PLS90_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK01436.1"
                     /translation="MPASPEPAAPPFIIGVDIGGTKCAVAKSTRDGQILAHETFPTES
                     MARTVALILEAVERLQPGGDPLFGVACGSPQDSARGIIQAPPNLPDWVDVPIIDHLTG
                     RFGGQGWLMNDANACALAEWFFGAGVGTRHMIYLTFGTGLGGGLILNGRLYAGACDNA
                     GEVGHFRLAEDGPVGVGKAGSFEGFCSGGGIAQRARDWARARAAGADDRPVTARDVAA
                     AADQGDLGAIAIFTETGRRLGQGLALIIDLLNPEMIVLGSIYVRCRHLLEQPMMEVLR
                     REALGPSLAACRIVPAALGENLGICSAIAVARYHLGNLPPASA"
     gene            complement(22631..23557)
                     /locus_tag="PLS90_03165"
     CDS             complement(22631..23557)
                     /locus_tag="PLS90_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01437.1"
                     /translation="MPASLAPTPRIRLHQADADAPWGPALLDSAERALEAAEARLGLR
                     LDQTARIVSLGSEREFHEYLQSRPAHVVAVARSRRNEIVIHREAFFRQTPVEQQRTLV
                     HEMVHLILGRRVRGELPGWLNEGLAMLVAGEGNYRSAWRVQVAGAFGALIPLEQLESQ
                     VAIGSELQELAYAQSLSVTRFYLERSWPESTAGPGGLNPAPLARELADPVEGPRLLTR
                     LWDVHFRRSLEAQWRRDHRNVWTWIAFLSGSGFIWLFAGVLFLLAYWRKRRFARQKRE
                     MFAAEEERDRELSLDIPPWEYEADESDEDRGR"
     gene            23975..25351
                     /locus_tag="PLS90_03170"
     CDS             23975..25351
                     /locus_tag="PLS90_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01438.1"
                     /translation="MIQVRQLSKRYMLYDRPLDRLADWLLPAALHRGHPFWALREMTF
                     DVAAGSSVGIIGVNGAGKSTLLKILTGTTRPTSGDYRIQGRVSSLLELGTGFHPEFTG
                     RQNIQFGAKMAGLTDAELAERYDGIVEFAELGDFIDQPVRTYSSGMLLRLGFALASSV
                     DPDVLIIDEALSVGDLHFQQKCLRRIREFHERGTTVLFVSHDPSMIQNFCNEAILLDE
                     GRMIDRGKPDQVIDYYTALLAEKYREGGAGVRIVRPRDDAPATDTAQAAEGPAPDAAA
                     DQNGWKAPPLHARGHRTGNFRAMITSVWLEARAVHGNSQIVVTGTPAVLHVRWIAREP
                     IPSATVGIMLRDRLGQEIFGVNSHYREVRMGPFQPGESVDVGFRMPINLGEGLYSITA
                     AVHEGENHIETCYDWVEHAFSLQVLPNPEERFTGLCRLPVEIEVQREPTPPAERQFAE
                     WVRSRLAS"
     gene            25354..27327
                     /locus_tag="PLS90_03175"
     CDS             25354..27327
                     /locus_tag="PLS90_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01439.1"
                     /translation="MRRYPRIVRVLRWLLLLIVVAGLLVYLIQLVRHGRTSYRHGMFA
                     IITGPYQWSWQVHPGLSLRWLGAYALVAWLVWGGRLRTLAASLPARMLIMTPILVLAG
                     YLTVSHGTGAPDIQPAFLLAVIVVAVAASYFIARLDDRPALPGPAPRWEAPLLYGLAT
                     AAGVGVAVLCWRLVYHGFPIMMDAQSQVVQARMLWQGRWVLPISQALRDVIEFPNSVF
                     TVPVYSQYPPGYIFLLIPVVALDLPPQTLNLAAGGGLVALTAWLARRLGGARAGWAAA
                     VLCAGSPFMILCGTAMNHATSALALLGTTCCLLPRLGRAEPRRWALRGLVGGLLLGWA
                     VSTRPLTGLAHALVWLAAWLAQLGELAWRRRSGRIVRPGQTLAAVLWRGLWVLLGLIP
                     PALLFMFYNLKTTGSPFIFAYEVSNPDLHRIGFGQATELHYTPADALHNSMASYLDLN
                     IKLFGWAVGSWTLILAWWLRTRLTRAELTLLGLIVSQSIFYGLYQYHDLILGPRFLFD
                     LLPYFAVLAALGLAPALRRGRRRGMVLALIVFAFSIVGIQRGINFWITKFRPVIERNV
                     QFAGFMDEIEPLEGPTVVVMDQPYNEVIGQYFQHPRYQHPLWFVFEHREAEARLLPEL
                     AGFHWVRFDDGNRPAPGPPPELPQFPQGGEPAS"
     gene            complement(27366..>28596)
                     /locus_tag="PLS90_03180"
     CDS             complement(27366..>28596)
                     /locus_tag="PLS90_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPK01440.1"
                     /translation="LPRATPALMRSVVDHCREARLAFRTVPNMEEVVHGQVEVSRLRP
                     VEIEDLLGRPPVVVRGAGPAESCLTGRRVLITGAGGSIGSELARQALAHGASELILLG
                     RGENSLYDIHQELSAPARERGAALRLVVGDVRDAALIEPLLAEARPAVILHAAAHKHV
                     HLMEAQPAEAIKNNVMGTRVLAEAARAAGVERFILISTDKAVRPTGVMGASKRLAEMI
                     VSALNRPGATRFVAVRFGNVLGSRGSVIPLFRRQIQAGGPVTLTHPEMTRYFMTIPEA
                     VSLVIEAGSQGQGGEVFLLDMGEPVKIVDLARNLITLSGLEPERDIPLVFTGARPGEK
                     LFEELLTAEQGREPTAHPKIFRSSQPAHDWEQLRPRILELEALAVLRDDAAIRRMLKE
                     LIPDYQPGGGGSEAAGG"
BASE COUNT         4662 a         9827 c         9323 g         4784 t
ORIGIN      
        1 tcggtgcggt tgccagacaa cggcccagct acaacataaa tcaattataa tcagtgcttt
       61 gtcattattt cgcgcatatt caagacgccc atcggcgatc gtatctcgca tctgcacgcc
      121 gcctgatcac ggcccttcag cgggaattta ttatgtggga aaatcgtgat gcggcggcta
      181 gactctgtcc cggatcctgg gggggcgaat gcgcggctgt cgcggcgtca gtggcggcga
      241 atcgctcagc gccggccttc agcccaggat tgaggaaaca cccgccggag cttattctgg
      301 agcgacgatt ggcggcaacg ggaggatcgg atgaacagca tcagttcggc gcgcgaacag
      361 gccgatcaac tgcgcgagtt gctcgaacgc atcgcccagc agcccgacct gttggacacc
      421 ctggaccgga tttgccggac ggtggtcgag gcgctcgccg ccggcggcaa ggtgctgacg
      481 ctgggcaacg gcggcagcgc gaccgacgcg ctgcatctgg ccgaggaatt gatcgggcgg
      541 tatcggaccg atcgcgcgcc gctgccggcg gtctgcctgt cggccgatgt ttcggccctg
      601 acctgcatcg ccaacgattt cggctacgac gcggtgttcg aacgccaggt gcaggcgctc
      661 ggccggccgg gcgacgtcgt catcggcttt tcgacgagcg gaaattcgcc caatgtgaat
      721 cgggcattcg gcgccgcccg cgcggcgggc gccacaacga tcggcatgct cggcaagacc
      781 ggcggggccg cccgcgacct cgtcgatctg gcgtgggtcg tcccgcacga caacacggcg
      841 cggatccagg aactgcacac gctggcgctg cacgccatct gcgaggcggt ggaagcgcgt
      901 ttcggcctgt cgtcaactta aataaggtgt ccatcgggcc gcggttgggg ccgatgcaac
      961 atccggcaaa gggggaggat gcgacatgaa cctgacacct cgtgagatcg tactgagaac
     1021 gctggcgttc gatcatccgc cgcgggcgcc gcgccagctc tgggtcctgc cctgggccga
     1081 gctgcatcac cccggcgcct tcgaccggat ccaggcggaa ttcccacccg acatcgtcta
     1141 cgccgatggc caccagcgcg aaccgcgcca ggtccagggc agtccctacg agcgcggggt
     1201 ctacattgat gaatggggct gccggttcac caatatccag cccggggtgc acggcgaagt
     1261 gtgcgatccg ctgatccgcg actgggacga cgccggcgcg atccgcttcc cgcgcgagat
     1321 gctgacgatc gaccccgatg ccgtcaaccg cgactgcgag gccagcgacc ggttccagct
     1381 ggtcccctat tgcccgcgcc cgttcgagcg cctgcagttc ctgcgcggct cgcaggcgct
     1441 gtatctggac ctgatgatgc gcccccccgc gatgctcgcg ttcctgcgca agctgcacgg
     1501 attttactgc gaatttctgg aactgtggtg ttcgcggacg caggtcgacg cgatcatgtt
     1561 catggacgac tggggctcgc agcgcaacct gctgatcgcc cccgagctgt ggtgcgagct
     1621 gttcaagccg ctctaccgcg actacatcga gatcgcgcat gcgcacggca agaaggcctt
     1681 catgcactcg gacggctaca tcgtggcgat ctatccgcat ctgatcgagc tggggctcga
     1741 cgcgctcaac tcccagatct tctgcatggg cgtcgaaaac ctccgtccgt tcgccggcaa
     1801 gatcacgttc tggggcgaga tcgatcgcca gcagttattg ccccacggca gccttgagca
     1861 gatcgacgcg gcggtcgaat cggtctacca tacgttgtgg cgcggagggg gctgcatcgc
     1921 gcagtgcgaa ttcggccccg gcggcaagcc ggagaatgtg cgccgcgtgt tcgaggcgtg
     1981 ggaccggctg acctcgccgg cgatgacgaa ataaggcgcc gcccgggggg atcccgtcgc
     2041 tcgtccatgt gaccagccgg tttcccctgt cctctcatcc cctttgtgag caaggccaag
     2101 aacgccgccc atgacgctcc aacagatcac gctccacgat gcggcgctcc cgcgccacga
     2161 cctggaagcc ctgatgcacg acatccagtc ggtctcggtg gcggtcctgg gtgatttctg
     2221 tctggacgtt tactggttca cggacctgtc ggcggcggag ctgtcgctcg aaaccgggct
     2281 ggagacccgc cccgtccggc gggctcatct gtcgctgggg gcggccggca acgtcgcggc
     2341 caatctgctg gcgctggggt gccggcgcat cgaagccttc ggcgtcgtcg gctgcgacat
     2401 ctgggggcgc gaaatgctcc acctgctgcg tgaaagcggc gtgggcaccc gcggcatgct
     2461 ctccgaaccg cgcgactggt ccacgctggt gtatgtcaag ccgcacatcc aagataagga
     2521 aaccagccgg ttcgatttcg gcaatttcaa ttgtctgccg gcgcagaccg cgcgaacact
     2581 gatcggcgcg ctcgtcgaac ggctcgacga attcgacgcc gtcctgatca acgagcaggt
     2641 gaccaatggg ctgcacagcg cggagctgcg ccaggcgctg gccgaggcga tccagcgcca
     2701 ccccgggacg aactggatcg tcgacagccg ccatttcccc gaggcctacc ccggggcaag
     2761 cctcaagatc aacgatctgg aggccacccg gttgctgggc atcgagcggc cggcgggggc
     2821 gctcgtgccg cgcgaggagg cgctgacggc ggccgaagcc ctggagcagc ggatgggccg
     2881 gccggtttac gtaacgcgcc gcgatcgggg cgtgatcgtc cacgacggcc gggcgccgca
     2941 ggagatcccc ggcctgctga tcctgggccg gatcgacccg gtcggcgcgg gcgacagcat
     3001 gctggcgggg atcgcggccg ccatcgccgc cggccgctcg cccgttcagg cggccacgct
     3061 gggcaacttc gtcgccggcg tcacgattca gaagctgtat gtcaccggca ccgcctcgcc
     3121 cgacgaggtg ctccggctgg gcgccgagcc cgattacgtc tatcgcccgg aactggccga
     3181 agacccgcgc ggcgcggtct atgacgacgg cgccgagatt gagatcgtca gccgccgctc
     3241 gcccgccggc cgccctaccg tcgccatctt cgatcatgac ggcacaattt ccaccctgcg
     3301 ccaggggtgg gaagccgtga tggaaccgat gatgatccgc gccattctgg gcgagcagta
     3361 tcagagcgct gacgagtcgc tgtatcatca ggtggtcgac cgcgtccgcg agtacatcga
     3421 tcactccacc ggcatccaga cgttgctgca gatgcagggg ctggtgcgga tggtccgcga
     3481 gttcagctgc gtgccggagg accagatcct cgacgagttc ggttacaagg cgatctacaa
     3541 cgaggcgctg atggcgatgg tgagccggcg gctggccaag ctggagcgcg gcgagttgca
     3601 ggtcgaggat ttcctgatca agaacgcctg ggccctgctc gagcggctgc gcgacgccgg
     3661 cgtgcggctg tgcctcgcca gcggaaccga ccaggaggac gtgcgccgcg aggcgcgcgc
     3721 gctgggttac gccgacctgt tcgacggcgg catctggggt tcggtgggcg acgtgaacaa
     3781 ggacgccaag cggatcgtgc tcgaccggat catgaacgag atcggggacg tcggcatggc
     3841 gggcctggtg acgttcggcg acggcccggt ggagatgcgc gagacgcgcc gccgcggcgg
     3901 cttcgccatc ggcgtcgcca gcgatgagat ccgccgctac gggctcaacc tggaaaaacg
     3961 atcgcgcctg atccgctccg gcgccgacct gatcgttccg gacttctcgc agctcgaccg
     4021 cctgctggac tgcctcaacc tgtgaggccc aacgtcgttc tcgtccccgc tcgccgatcc
     4081 cgtctcatgc tcgcggcgtg cgcgaagctt cgccgccggg tatgatgacc gggccgaggg
     4141 cgtcggttgg cgcccccgcc aaatttccct gtaaaggacg acctccccag atgccgtacc
     4201 cgcaactgga tcgctcgcgg gtcaacatgc ggccgctgcg cgagcgcaag aataagaagc
     4261 ggattgaacg ggaccacatt gcgctggata cccagccgcg agggctcacg ccgagcgacg
     4321 aacgcctgat cgccgaagtg gccgagcgga tcgaggcgtc ccgcgcggcc gaccggtcgg
     4381 tgatgctggc cttcggcgcc catacgatca agaacgggct cgccccggtc ctgatcgagc
     4441 tcatccgccg cggctgggtg acgcacctgg cgaccaacgg cgccggcgtg attcacgact
     4501 gggaattcgc ctacctcggc gagtcaagtg aggacgtgcg cgccaacgtc gcccgggggg
     4561 aattcggcaa ctggcacgaa acggggttct tcatcaacct ggcgatcaat gtcggcgcgt
     4621 gggagggccg gggctacggc gaagcagtcg gcgcgctgat tcagcgcgag ggcctggaga
     4681 tcccgacgcc ggcgcggctg gagcaggaag tgcgcgctgt attgaaggcg gaccccgcgc
     4741 gcgcggcggc ggcagccgaa ctgctcggcg tcctccggcg gttcgagctc gagccggggt
     4801 ggctcgccgt gccgcacccc ttcaaggagt tcagcgtgca ggccgccgcg tttgaactgg
     4861 gcgttccgtc gaccgaccat ccgatgttcg gacacgatat tatctacaat cacccgatga
     4921 acaatggggc gctgctcggg cgcgccgccg aacgcgattt cctgcgcttc gccgccggcg
     4981 tcgaccgcat tgccggcggc gtctatctgt cgctcggctc ggccgtgatg agcccgatga
     5041 tcttcgaaaa atcgctctcg atggtgcgca acctcgcgat gcaggagggc cgccggatcg
     5101 acgatcatta catgctcgtc gtcgacctcc agccgtcgca ctgggactgg cgccagggcg
     5161 aaccgcccga agacaacccc gattactacc tgcgctactg caagacgttc gcccgcatgg
     5221 gggggacgat gcagtatctc tcggccgaca accgggattt tctgctggcg ctgcatcgcg
     5281 cgctgagcgg ccgccgggcg ccgggtgagt aatccgcgcg cccgcaaccg acccgccgcc
     5341 cgtcagacct ggctgaggac cggcgtcttc ttcatctcgc gccggcccgc gcactggatg
     5401 gcttcggcat tggccagata ctgctccagc ggaaccagtt cgtaggcgcg gatcagttcc
     5461 tgcacatcga cttcctggtc gcgcggcaga atccgccacc acgcctcttc gggatagatc
     5521 ttgatctcga tcgcgcggcc caggtcggcg cgcagccggt cgatcttctc gaaatggaaa
     5581 tcgaggtgaa acagcgggct gtcgaggcgc agggtcgccg ccacccattt ctggtaaatt
     5641 gcttcccagt tcaggtcgag gccgtcgatg tcgatgatgc ggcagctcgt gcggcaggtg
     5701 gcggcgacga tcgggacgtg gaacagcgtc gccatcgaat ggaggacgcg gccggccgga
     5761 taggccgagg aaaagaagat aatctccgcg ccggcctgct tcagttcatc ccagttgcgc
     5821 caccagttcg cgtcgaagca gatctggcag ccgattctgg cgaagtcggt ctcgatgatg
     5881 gtcggcgcgg gatcgccggg aataatgtag tcctcgacct cgcagccggg gccggcggtc
     5941 ggatggattt tgtcgtaacg gccgcaaatc tcgccgttgc ggtcgaggac cacgcacgtg
     6001 ttgtagatgt gttcgccccg gcgttcgatg atcgggcaga tgatgtagca cccatgctgg
     6061 cgggccttct cgcgcatgcg ggtcacggtc gggccgtcgg ccgtctgcgc catttcggga
     6121 tgacacgagg agacgagccg cgtgtcgaac gtctcggtca tgcagataat gtcgggctgt
     6181 tccgaaccgc tgatctcatc gatgacgtcg ctggcgtagt tcaggttgtc ctcgagcgtg
     6241 cgcggctgct tgtcgaagcg gtcgatcgtg aacgccgcga cgttgacttt gcgtatcatg
     6301 gtgcgtgtcc cctttgcgtg gtgcaatagc ccgcggaggc agtagcttgg taaccgatcg
     6361 tttatatcct accccggccg cgtttccccc acaagcgaaa ttccggcgtc gccgtaagcc
     6421 actcgttctc tgctcgaagc ctctcaggca atccccttcc gccgggcttc cccttttcgc
     6481 ccgccgcgga taaaatgatc actccgcgcg gatcggcccg cgcgccgcgc gccgcatccc
     6541 ccgcccttgc cggcctggag ccgccacgat gccccgcctt tgcattccgc tcgtcctgac
     6601 cctcgtcgtt gcgctcatcg cgccggctcc cctcccggcg caatcggccg gttcggacat
     6661 gccgacggag atcaccgaac ggctctggcg cgatcccggt cccgagcagg acggccccgg
     6721 cgcgccccga ctgatggtgg tctatcccgc cgccgccggc gggccgtatg acggcgaggc
     6781 gatgcgctac gccggccgcg tcgaccccga ggacgcgacg gtgacgctca acggcgacga
     6841 attgcaggtt tatcccggcg gcgtgttcac cggactgcgc cgtttcggcg acgccgagag
     6901 cgagacctgg accttccgcg ccgccgccgg cggcaaaacg accacggtca gccgctcgct
     6961 ccgcatccgg cggccgacgc ctccgcccgc ccattggccg ctgcaattcg cctcctggcc
     7021 ccactcccct tccgggctgt tcgtcctgcc cgaaggcgaa gagctcgaga tcaaactgtt
     7081 tgccagcccc ggccacagcg ccgaataccg catcggcgag gccggcgcct ggcgcgcgat
     7141 gacccccgcc gagccgcacc cccggctggg aggcgtctac accgcctcac tgacgccgcc
     7201 gctgctggac gacgccgggc cgcgcaccgt tcacttccgc ctcagcggca gccacgaggg
     7261 ccggtccgac cgcatcgagc ggctatcctc gctgcaggtc gcggcgcggc ccgacggcgt
     7321 gcgctacgcc ggccgcgtca cgaactacct ggccaattac ctcaagcact ccgaccccga
     7381 gaactgggac cgttatggcc agctcgtcaa gggcacgccg tttccggtca cggaggtccg
     7441 cggcgaccgc tgcaaggccg atttcggccg gggagaaacc ggctggctcg agttggaaca
     7501 tgtcaagatc gactgggaac cgttcgccgg gcccgcaccg cggctggacg agacgcgcgt
     7561 cgtcgtcacc agcgacagcc tgacgctcgc atggcccgac gccgcccagc ctgtcctcta
     7621 cgtattcgac gaccggagtc acgccggccc cgagcatgac gtccggctca gcctccccgg
     7681 cgccgacgaa agcatgcgtt gcgatccgcc ggactccctc cccggcgggc cggtcgactc
     7741 gctccagttc cgcccagccg agggcgcggc ggcgcccgcc ttcctcattt cgctcacccg
     7801 gccgctatgg ggttactcga tgctggcggg ggcgagcgac gaggccgacc agccggggct
     7861 gcgcttcgtg ctgcgcacac ggccgcgcct gccggcggcc accgccgcca agcccctcgc
     7921 cggcgcgcgg atcatgctcg atgccggcca tggcggcgac gacctcggcg ccatcggccc
     7981 ctcgggcctc gtcgaggccg atctcaacct cgtgcaggcc gcctgggtgc agacctatct
     8041 ggaggaactg ggcgccgagg tcgagcagac ccgccggtcc cacgcgacgg ttgacctgga
     8101 cgaacgcgcc ctggccgccc accgctggga tcccgacctg ttcgtcagcc tccaccataa
     8161 ttcggtcggc ttcaccagcg atccgctcgc cgacagcgga ccgattgtct tctatcacta
     8221 tgaccacgcg cggccggtcg ccgagaccgt cggcgaggcg ctcaccagcg ccatcgcccc
     8281 cgaccggtcc ccccgggtga acagcgccgt gttccgcgtc gcgcgcaacg tcctgcttgc
     8341 cccctcgatc ctgatcgaga ccgggtatgt ctgcaatccg cacgatgaag cggcgctgcg
     8401 gcggaccgag agcatcaagc aatcggcgcg cgcaatcgcc gaggggattc gcgcggcgct
     8461 gacggagccg gccgattgag ccgtggcgac aggtcgggct caagattccc cgcgccccct
     8521 ccgatgcgta ctgggagccc catgctgccc cgtgcgcccg aagctcgccg tcggagtccc
     8581 gtgccatgcc cgcatccgcc tacaactgtc actacgtcca cgccacgggg acgctgcaga
     8641 tcgtcctgct gcccggcttc cgccaggaat actgggatga actgatgcat tatgtggaaa
     8701 acaccgaccg gtcgggcaat taccgctggg atctcgacct gcggcggatc gaggtgatca
     8761 acagcatgat gctcggcctg atcgtcggtc tgcgcgcccg gctcgacgcg cgcggcggca
     8821 acctgcgcgt gctgatcgcg ccgaattcga aagtcgagca actcctccaa ttgacccgct
     8881 tggaccagat cgtcgagacg gtgaaagcct gagccgccaa cgaggcgagt cgtctcccgt
     8941 cctcgtttac gataatcaac ccgcctcaga acgtgtcgcg gcgatcaagc gacttggcga
     9001 ccaggatcgt cacgtcgtca aaggcctcgg cgtcgcgcga gaaccggttg acttcctcca
     9061 gcatgccgcg gcggaaatcg gccgccggct cgccgacatg ccgccgcagg tagttgatga
     9121 tccgctgctg gccgaacatg tcgcccttga cattctgagc gtcggtcacg ccgtcgctgt
     9181 acatcagcag catgccgccg gggggcagtt ccagggtctc ggtctcgtaa ttgatccccg
     9241 gcatcacgcc gagcagcgtt ccgcccgatt ccagcgtccg cagtgtgccg tcggccaggc
     9301 agagcagcgg gtgcacatgg cccgcgttgc agtaggtgaa ctggcgcgtg tgggggtcca
     9361 ggacgcccag caccattgtg acgaacatgc tgttggaggt gtcgcggcag accatctggt
     9421 tgatgcgatc gacgatcgtc gcaagcgttt cgttgcccag cgagcgcacc tcgatctgca
     9481 cggcggtgcg gatcgccgcc agcagaatcg cggcgggaac ccccttgccg gtcacgtcgg
     9541 cgatcgccag cacgagccgg ccgtccggca gcgagatgac gtcgaagtag tcccccccga
     9601 ccatataggc cggccgcgtc gtgccggaga tttcgaagtt gtccagctcc ggcatgctct
     9661 tgggcagcag gtgttcctgg atcgtgcggg cgatctccag ttcgcgggcg cgggcgtgct
     9721 gctcgagcag ttggctgtgg aaacaggcgt tggcgatggc gacggccgac tgcttggcga
     9781 tgcccgccac cagtttgagg tcctcctcgc cgaacgagcc gctgggccgg cgccggtcga
     9841 ggtaaatcac gccgaggact tcgttgttga agatcagcgg ggcgcacaga gccgaatgga
     9901 tccgctggat gacgatcgaa tcggaggcgc ggaagcgcgc gtcgcgcggc gcatcgctga
     9961 cgatgatcgc cacttcgtcc tcgaccgact tggtgatgat cgagttggag atgatgatgt
    10021 cgtcggtcga atcgggcggg cgccgcaccg cgcgcggcag caggatgccg tagcgttcgt
    10081 cgtggagcag gatgcccgag cggtccggct tgagcgtctc ctgcaccaga tcgatgatct
    10141 tctccagcag ctcgtccagg ttgaggacga tgctggtgac gtcgagcagg cggtagagca
    10201 cgccgagctg gcggttgccg gcatacggca tggtgtagag cggcgcggtc gaatcgcccg
    10261 agctcttctc cgacatgcgc aggatctggc tcgggacaag catgccgtgg tcgtcgagca
    10321 cgcgcacgcc gctgctcgtt tcgacatggc cgtcgtgtgt cggttccagc tcgaaggcga
    10381 agacggtatc gccgatctga atccggtcgc cgtggcccag cacctggtcg cccgggatcg
    10441 cccggccgtt gagcagcgtg ccgtttttgc tcccgaggtc gcgcacgagc cactgcgagc
    10501 cgttcaggat gaagagcgtg tgattgcgcg acatgcgcgg atcgatgatc tgaatcacat
    10561 tgtccggtcc gcgcccgacg gaaaccacct tgtcgcgcaa cggaaagacc atccccttct
    10621 gctcgccttc ctcgacgacg agtcggcagg gggcgtcatc ggacgaatgt tgcgcgcggt
    10681 ccagcgtctg attcatgcca ctgacctttg atcgacatgc ggggggccgg cgggggggca
    10741 ggttcaccgg tgcgcgcctg gcggccctcc ccggctactg ctggtcatcg tatcggaaga
    10801 atcccgattt tccatcagtc tgtatccagc gggcgaaggt caagcggcgc gccgcgcgcg
    10861 aagctggtcg agcgccaccg ccagcacgat gatcaggccg acgagaatct cctggatgta
    10921 gttctcccag ccggcctgcg tcgcgccggt ggccagcacg ctcatgatca atgccccggc
    10981 cagcgtcccc aggatgcggc cctcgccgcc catcaggctg ccgccgccga tcaccacggc
    11041 ggcaatgacg ttcagctcca ggccgacgcc gaccgaggga tcgccgcgcg tcagccgcgc
    11101 cgcctgcacc acgccggcca ggccggccat cgcgccggcg acgacgtaga ggctgatttt
    11161 cagccagccg atccgcaggc cgcacagccg ggcagtcggc tccgagctgc ccagcgccac
    11221 cacatggcga ccgtagaccg tccggtgcag cacgaggccg aagacgagcg ccaggccgag
    11281 cgcgagccag acgctcggcg ccaccagcag ccagctccag ccctccggca ggtagggctg
    11341 cacccattcg ccgaaatcgt aggtgatgtt gatcggaatg ttggcggact gccatttggc
    11401 ggccccgcgt gcgatcgaga gcatccccag cgtggcgatg aacgggacca cgcgcaggcc
    11461 gacgatcgcc agtccgttaa ttgctccgca gagcatgcca atgccgatgc ccgcaccgaa
    11521 cgccgcgcac caggcggcgg gcgtcatcgc gccgtcgacg ccgatcaggc cggcaagcga
    11581 cttggcgcag acgacgctcg ccagcgccac gaccgagccg gtcgacagat cgatgccgcc
    11641 gccgatgatg accagtgtca tgcccagcgc ggcgatggcg acgatcgtcg tgtcggcgag
    11701 gatcgtctgg acgttatacc agtcggtgaa tccctcgccg acgatgatcc ggaagatcac
    11761 gacgatcagc cccaggccga acagcggccc caaggccgaa gccaggcggc gaagggacgg
    11821 cgcgtgaggg agcagcgaca tcggattcct cccggaaatt tgccattatc gggatcagac
    11881 gcaagcctgc aagcggcaac gcccggcttg gtgattcgcg ttttcgatca tccttcctcg
    11941 cgcggcgggg gctcgcccag cgtcgcggcg cgcagcagcc gctcctcggt ccactgcgcg
    12001 cgcggcgcca gcgccaccag ccgtccgcga tacatcacgc cgatccgatc gcagatcccc
    12061 agcaactcgg ggttgtggct gctcgtcagc agaatcgctt ttccttcggc cgcctcacga
    12121 ttgagccact cgtagatcag caccttggag ccgacgtcga tcccgcgcgt cggctcgtcg
    12181 agcagcagca cgcgcgcctg gctgaagcgc agccgggcca gcgccacctt ctgctgattg
    12241 cctcccgaga gatccagcgc ccgctggccg atatcgcggt aaaccgtcga gagcgcgtcg
    12301 gcggcgggga tggcgaggcg gcgcaggcga gcgcccgaca gccagccgat gcgcgcgatc
    12361 cgccccagtg cgccgaggcc gatgttttcg gcgaggctca gccgcagggc caacccctcg
    12421 ttcttgcgat cctcgctggc gaagcccagg ccgagccgga tccggcgatc cgcccccgcc
    12481 gtcagcggcc gcggccgatc gccggcgatg aggacggcct cgcccgacgc cggcggcagc
    12541 agaccgtaga tcgtccgcag cagttcactg cgtcccgagc cgaccaggcc gccgatgccg
    12601 acgacttcgc ccgcgcgcag cgacaacgaa acgccggccg gccggccgcc ggggcccgac
    12661 agattcgcca cggcgaggac ttcggcgccg ggcgtccgcg ccgggcgtgg aaagagctga
    12721 tcaacgcggc ggcccaccat cttcgccacc aggtcatccg gtccgatctc tgcgacgcgg
    12781 ccgctatcca ccgtacgacc gtcgcgcatg accgtgtagc ggtcgcacag ttccatcact
    12841 tcctccagga aatggctgac atagaggaag ctgacgccct gatcgcgcag ggcgcggatc
    12901 cggtcgaaca gatggcgggc gtcggcctgg gtcagggcgc tcgtcggttc gtccaggacg
    12961 atcagttgcg cgtcggccgc cagcgcgcgg gcaatctcga cgaactgctg ctgcgccagg
    13021 ggcagtcgcc cggccggcag gtgcagcggg atgtgcgact gctcgagcag ggcgagcagc
    13081 gcgcgcacgc gctcagcgga cggcctccgg cggatccagc cggcccgcgc aagttcctga
    13141 cccagcagga cgttttcggt gacggtcaga tggggcgcga tcgccagttc ctgatggcag
    13201 gtggcgatcc cgcgccggcg ggcatcggcc ggccgcgccg gctggtaggg ctggccgcgc
    13261 cacaggatcc ggccgccgtc ggggcgcagc gcccccgcga gaatcttgat catggtgctc
    13321 ttgccggcgc cgttctcgcc gacgagggcg tggatttctc cccggccgat gctcagctcc
    13381 gcgccgtcga gcgcgtgcgt cgcgccgaaa tgcttctgga tggcgtgagc gtggaggaga
    13441 ggggattcgg gcatggaggc agaggttcgt ccctcgtgat cgtcctcact cgaaaatctg
    13501 tccgctaatc gcgggatcgc ctggatcggt tctgccgtcc ctacgggact ctgtcgccag
    13561 gggtatgcct gtttcccgcc actggagtag cgggctgaag tctgccgtcc ctgacgggac
    13621 tgccctgttg aagaatccgg caaggccaag gctgttcgaa tcggcagttt gcccccccat
    13681 ttcatccctc gttgtgaagc gcgggttcat gagtctcgca atcggattcg aaaacgaggg
    13741 acgagagacg aagggagggg ggactggcag gaacccgcgt tattccttca ggtactcctc
    13801 cagcggcggc gcgatgactt cctgaatctc gggcagctcg aagttctccc gtgtcaccag
    13861 cgcgacgccg gtgtcgatcc gttccgccac ctcgtcgccg gcgagggcgc gcgcggccgc
    13921 catcacccct tcataaccca tcaggaacgg attctgcacg accagcccgt cgatctgccc
    13981 cgccttcagg gcgtcgagca gtttggggct cgcatcgaac ccaatgaacc tgaccttgcc
    14041 cgccaggcca tggttgcgca gggcgagcag catgccgaac gtgctcgatt cgttcggaca
    14101 gaaaatgccg tcgatctgct ggtccttcat cgcgttgagc aggctgttgc tcttctgcaa
    14161 cgcttcgtcg gaggtggcgc cggcatactg gttttcgctg atgaccttga tctcgggaaa
    14221 ctcggcaatg gcttttagga agccggcctc gcgcgcctcg gtggaggcgc tgccgacggc
    14281 gtagcgcagc atgatgacat ggcccttgcc gccgagcagc cggcccagtt cgcgccccgc
    14341 caactcgccc ccccgctcat tgtcggtggc gacgaaactg acaatctcga ccccctcgcg
    14401 ttcgatgtcg ctgtcgatga tgacgacggg gatgccgcgc cggttggcct gaatgacggc
    14461 gttgcgcagc gaacgggcgt cgagcggcgc cagcacgatc gcatcggttt tcttcgccac
    14521 gtgcgattgc acgatctgga tctggctgtt gcggtcgtcc tcgcgcagcg gcccctgcca
    14581 ggtgatcgca acctggtcca gttcgcgatc ggccttggcg gcgccggcgt gaaccgcctg
    14641 ccagaaatca tgcgtcgttc ccttggggat gacgacgatg ttggaacctt cgcccgcctg
    14701 cgcgtgaagc ggaggcgcgg cgaacgcgac cagcgccgcg acggcggcca ggcgccacac
    14761 ggagcgcgcg agcgaatgga aatgaacggg catgattgac tctcctctcg gcacggattc
    14821 acccaagccc gagcatttta agtcgccgcc caccgggctg caagcgatcc ggaccgactg
    14881 atcccgatcg ccgttcgcga tcatcgcgct tcccccgcgc ccctggcgga attgccgact
    14941 tcggcatgcg gcccgcttct tgctcacagc agcttaaacg gccgggcgct ccgccccgcg
    15001 cggcgcggcc gcccgaccag ttgccccatg cgaggtgcat catggcgaag tcaacgacca
    15061 aacagattgg ccggcggcgg cgagagccgg ttctgccaac tcgtcagccg acacggcgca
    15121 tcatcatttc cccacgggta atttccattg gaatttttgg ataattcaaa cgacgcaccg
    15181 tccagtcatc ccgaggggct tactccccgg ggttcgatcc aaacgcgaag agggtaatta
    15241 caatgagtat cgtcggcatg atcctgttga ttctgttgat catgattgtt ctgggctcgc
    15301 tccctgcgtg gccctacagc cgcggctggg gctactatcc cagcggatcg gtgggattga
    15361 tcctcatcat cgtgatcgtg cttctggtgc ttggcgtgat ctgagcgtct gaaccgcaat
    15421 ccccctgttg taaactcccc gcgaggcggc gtcggctgtt tttacaaatg gaagtattcg
    15481 agcacgtgat tcttgggggc ggcgtcgccg ccggccacgc cgcccgcgag ttcgtcaagc
    15541 ggggcgtcaa gccgggtcag ttggcccttg tttcggccga ggcctcgttc tgctacgaac
    15601 gcccaccgct ttccaaggcc ctgctggcgg gcacgaaacg gcccgaggaa acgctgatca
    15661 gtccccccag gttctatgcg gaaaacggca ttgcctttta tcatgaatgg cgcgccgtcg
    15721 gcatcgactt cggccggcgg ctgatgatga acgatcaggg cgagacgatc ggcttccgca
    15781 agctgatgct ggcgaccggc tcgcgggtgc gcaatctggc catcgccggc gccgatctgc
    15841 tgggcgtgca ttacctgcgg acgctggagg atgcgcgggc gatcgtcggc gcgctgcgcg
    15901 acgtgcggcg catggtcgtc atcggcggcg gtttcatcgg cgccgaggtc gcggcccaga
    15961 ccgcccgccg cggcatcaac acgacgctgg tcgcggccac tgatcacctc tggccggggc
    16021 atttcacgcc cgaaatctcg gctttcttca agggctactt tcagagcaac ggtattcatc
    16081 ttatcttttc cgccaggccc gcggcgcttc acggccccga gcatgtcacg cgcgtcacgc
    16141 tggagaccgg ccagtcgctc cccgccgaaa tggtggtcgt cggcatcggc gtcgtgccgg
    16201 agctggagct gtatcgtcag accggcctgc agctcgacgc gggcgtcctg gccaaccagt
    16261 tcctcgagac caacatcccc gatgtctggg ccgtcggcga catctgccag tatgaggatg
    16321 tgctgttcgg caagcgccgg cgcatcgaac actgggataa cgccaaaagc cagggcgaac
    16381 acgccgcgcg cgcgatgctc ggccggcgcg aaccgttcat acacgtgccc tacttctttt
    16441 ccgacgtgtt cgatctgtcg tgggaattct ggggcgacac cgaggaggcg gatcgcgtgc
    16501 tctaccgcgg cgatctgggg cgcggcggca tcagcgcgtg gtggctgcgc ggcgaccagg
    16561 tgcacgccgc gttcgtgatg aaccggcccg atgaagaacg gcgcgccgcg ccgctgctga
    16621 tcgcgcacgg cacgccgatc aacgccgaat gcattgccaa catggaaatt ccaccggcgc
    16681 aatggacccc ggcgcagaac tgcgaaactg gtccctctct tgcttagtaa atcattaacg
    16741 ggacggcgcg cgaggaactc cccgcaaatt catctcctgt cccgcttcaa tcatccctgg
    16801 ggtgattcct tcggaggtta aggaccatgt tatactgggc tctcgtgttc ttcattctgg
    16861 cgctggtcgc cggtctgctc ggctttggcg ggctctacgc cgcgttcgcg acggttggca
    16921 agattctgtt cgtcatcttc ctgatcgtct tcctcgtctc gctgatcatg ggtctggtcg
    16981 gcggacggcg agtcgtctga gcctctgctt gcgcaccccg ccctctcgtc agggcggggt
    17041 atttgttttt cagcggactg ctggggaaat cccccaccga tccgaccgca ttcgttccca
    17101 cccgcgggtc ctgtttcacg gaaattgtcc gtcggctgct tccgttaaca aaatctgcaa
    17161 tacacattct cgggctgatt tgctaacctg acataccgcg gcccttacaa cccagggcgc
    17221 cgcatggggg caagtcgaac acgagactga caggggaatc gctccccgcc aggcgccgcc
    17281 gacaggcagc atggtttaga atccccgcca ccggggcggg gagcatcgca gcgcgcagcg
    17341 gcgcaacaac cgggctgccc atcatcagga agcaacgacg atccggatga attcgaagat
    17401 caccgttacg caggtcccac tccagcaact gatcgacgtg ctcgacgtgg gcatcctggt
    17461 gctggggcgc gacggccagc ccgatttttc caatgcgccc gcgcgcatgc acttcggctg
    17521 cacccgacac gatgatctcg tcgaacgctg gcagcagatt gaccgcgagc tgaatgagat
    17581 cccgaccgac ggccagggcg ccgtcgcctg caccgtgcag ctcgaagtcc ccggcccggt
    17641 gcgcgcccgc cggctgcaac tggaattcta tccgctcaac catgaggact gcgaaggcca
    17701 gatcgtcctg atccgcgacc gcgaaatgct cgacgcgatg gaaaccgacc tgcgcctggc
    17761 ctcgcgcttc cgcggcctga cccggctcta ccgcgccatc gcgcacgatc tgaaggcccc
    17821 gctcaacgcg atggtgatca acctggaact gctcaaggag acgatcaatc tcccccatcg
    17881 cgatgcctcg acgcaggaac gcaaggaccg ctatgtgaac atcctgatgt cggaactggg
    17941 ccggctcaac cgcacgctgc agctcctgct gacgcaggcc gatctgtcga cgaagtccag
    18001 cgaacggatc gacctgggcg agatcctgct cgatgtggtg accctgctga ccccgcaagc
    18061 caaacagcag gagatctcgg tcaattatca cctgcccgag gagcccgtgc tcatcatcgg
    18121 cagcgaggac tggctcaagc aggcggtgct caacatcacg atcaatgccc tggaggcgat
    18181 ggatcgcggt ggacggctga cgattcggct ccatgtcaag gaacacgtcg cgacgctgct
    18241 ggtggaggac accggctcgg ggatctcgca ggaagacctg gaaaaaatct ggaacatgca
    18301 tttcacgacg aagtcatccg gaacggggat cgggttgtac gtcagccgct cggtgattca
    18361 gtcgcatggg gggagcatcg acgtgatgag cgtccccggc gaaggcagcc tgttccgcat
    18421 aacccttccc accgccggaa aagagaactg aggtagacga tgcgcgcact tgtcgtggaa
    18481 gacgatgcca gttgccagac ggccctggcc gaactggtcc agatgaaggg cttcgaggtc
    18541 gtgacggccg gcagcatcaa ggaagcccgc agccagctcc gcaagaaggc gcccgacctg
    18601 gcgctggtcg atctgaatct gcccgacggc agcggcatgg gcctgatcaa tgagatcgat
    18661 gaaaagtatc ccagcgaggt ggtagtcatc accggcaata ccaacctgga gaccgccgtc
    18721 gaggcgatgc agcagggggc gcttgattac ctgaccaaac cgatcgatgt gaccaagctg
    18781 aagaagatcc tcgacgatct cgcccaggac accgcccggc gcaccgacaa cagcctgcag
    18841 gccaaatcca gcgcgatcct gcgcgccagc cagatggtct tcgagtcccc cgcgatgcac
    18901 gagatcgtga cgctggtcac gcgcgtggcg gccaccgagg ccagtgtgat gatcatcggc
    18961 gagagcggca ccggcaagga actggtggcg caaatgatcc atgacaaaag cccgcgcgcc
    19021 gacgggccgt tcctgccgat caactgcggc gcgatcgcgc ccaacctgat cgaaagcgag
    19081 ctgttcggtc acgaacgggg gagctttacc ggcgccgcga cgcgccacct gggctacttc
    19141 gaacaggcca gcggcggaac gctgttcctc gatgaagtga ccgaaatgcc cctcgaattg
    19201 caggtcaagc tgctgcgcgc gctggaaacc agccagatca tgcgcgtcgg cagcggcaac
    19261 ccggtcaatg tcgacgtgcg cgtcatcgcc gccaccaacc gccagcccga gggcgccatc
    19321 gaggacggca agctgcgccg cgatctgtat taccgcctca acgtcttccc gatcgaaatg
    19381 cccccgttgc gcgcgcgccc cgaggatatc gtctgcctcg ggcgccactt cctcgcccag
    19441 ttcaacgccg cctacgagac caaaaaggtg ctcaccgacg aggggatgga aaagctctgc
    19501 tcgcattcgt ggccgggcaa cgtccgcgag ctgcgcaacc tgatctaccg ggcgttcatc
    19561 ctcgccgacg acgagatcgg ccccgatgtc atcaacttcg agaaagccgc caacggccat
    19621 cccggcgcga gcgtcgatct gaacatgaaa gtgggcacgt ccatcgccga tgccgaacgc
    19681 cggctgattc tggcgacgct cgacgcctac cagggcaaca agaagaagac ggcgcgcgtt
    19741 ctgggcgtca gcctcaagac gctctacaac cggctcaacg agctgaagcc ggaaccggcg
    19801 cccgccgcca aaccggcccg gtcccgatga agggctgaag gctgtttttc agggttggtt
    19861 cgataccgag agcgattccg atcccgatcc cgattgtggc gtcggcgtgt cgtcgagtcc
    19921 ccggctgttt acgattgaaa gaagatcttg gcttgcggtt agattgaagc acttccgctg
    19981 tcatcgggaa gaagtcatcg tgtttccaac acggaaggca gcccatcgga accgcgcccg
    20041 gcgtttgttg gcacaaatgc tgctttgggt gcggtcacag ccgatggctg cccggccggg
    20101 aggaagtgcc gttggccatc cgcgccaccc ccttcccccg cgattccccc tgaagaaagg
    20161 ttgattgaaa tgctgactca cttcacggtg gccttaaggc gtgtgctgat gctgttggcg
    20221 ctgctcttcg ccagcgtcgc ggtcatgccg accctcgtgg cctgcaacgg cggcgccgag
    20281 gacaatctgg aagacgccgg cgaaaacctg ggcgacgccg tcgaggatct gggcgacgcg
    20341 gcggaggatg ccctcgactg atttgatccg ccggcggcct tcccgctggc ggcacgggtc
    20401 ccttcaggcg gacagcctgc ccgcccgggc gctgtccgct ttttcattcc accgccgcgc
    20461 cggccatgcc ggccggctcc caccgcgcca gctcccggct gcgcgcgatt cccagccggt
    20521 tccatccgcg gccgccgaaa tccgcgcgcc gctgcccgtc gctgcccgca taaaacaggt
    20581 agaaccagcc gtcggtcgcg cgccggtcca aaagccaggc gccgttggcc aggatttccc
    20641 ggttgaagtc ctggaccggg atgtccagcg cggtcccgtt ctcccagcgc gagaatctca
    20701 gccccgcgct ggtcgattcg ggccgctcga acagatacgg ccgatggcga tcctcgcgcg
    20761 ccgccgccag caggcgccac ccctccccga tgcgcaggac ctggaagctg gcgaaatacg
    20821 gccggtgcac gccgtcgacc gtgctcagct ccaggtatcc cggtcccagc cacgcaaagt
    20881 cgccgacgag ccgctcgcca tgggccacgc gcggcagccg cccctggttc cagatcagaa
    20941 ggtggacctc gccgaacggc gccagggcgg ggtcgaccgc ccgcgcctcc ccagtcaggt
    21001 tggccgccag ggggcgggga ctggaccagg ccatcaggtc cggcgactca caccagaaga
    21061 gcgcgttggg ctgctccggc gcgtcgcccc acgaattgcc gaccaggaca tagcggtttc
    21121 cgtcgtgcac gacatagggc gaagcggtcc cgcgccaacc gcggctcgcg ccgtccagcc
    21181 ggttcacctc ggcgaatcgc accagatcgg cgcttgtgta ctggacgatt gtgcagttcg
    21241 gcgcgtcccc ggattcctgg aaggcggagc agaagatgtg gaacaagccg tcggcctcga
    21301 cgacgcagaa atcgtgcagc gattgatcgt gatcgccgag gatcggattg cgcagggcct
    21361 cccaatccag ctcggccggc gccgccccgg cggcgagcgt gaagcaaagc aggcccgcgg
    21421 ccgcgacgcg acgcggaaga cctaccaatt gtgatagccg ctcagccgtt ttcatcgccg
    21481 ttccgtccct ttcgtccata gttcggctct cgtcctcgtc ctcggctctc gaaaacccgt
    21541 cgattcgatc ccgatagcga tcccggtccc gctaccgatg gaaggagtcg catcccgccg
    21601 ccgggttggc tatgctcggg gcaattcttg tctgcaagga gtcacgcccc atgcccgcct
    21661 cgcctgaacc cgccgcgccg ccattcatca tcggtgtcga tatcggcggc accaaatgcg
    21721 cggtggccaa gagcacgcgg gacggccaga tcctggcgca cgagacgttc ccgaccgaat
    21781 cgatggcgcg caccgtcgcg ctcatcctgg aggccgtcga gcggctccag ccgggcggcg
    21841 acccgctttt cggcgtcgcc tgcggcagtc cgcaggacag cgcccgcggc atcatccagg
    21901 cgccgcccaa tctgcctgac tgggtcgacg tcccgatcat cgaccacttg accggccggt
    21961 tcggcggcca ggggtggttg atgaacgatg ccaacgcctg cgccctggcc gagtggttct
    22021 tcggcgccgg cgtcggcacg cgccacatga tctacctgac gttcggcacc gggctgggcg
    22081 gcggcctgat cctcaacggc cggctctatg ccggggcctg cgacaacgcc ggcgaggtcg
    22141 gccacttccg gctggcggag gacggcccgg tcggcgtcgg caaggccggc tcgttcgagg
    22201 gcttctgcag cggcggcggc attgcgcaac gggcgcgcga ctgggcgcgg gcgcgggccg
    22261 ccggcgccga tgaccgcccc gtgacggcgc gcgacgtcgc cgcggccgcc gaccagggcg
    22321 atctcggcgc catcgccatc ttcaccgaga ccgggcgccg gctgggccag gggctggcgc
    22381 tcatcatcga cctgctcaat cccgaaatga tcgtcctggg cagcatctac gtccggtgcc
    22441 gtcacctgct ggagcagccg atgatggagg tgctgcgccg cgaggcgctc ggcccgtcgc
    22501 tcgccgcctg ccggatcgtg ccggcggcgc tcggcgaaaa cctcggcatc tgctcggcga
    22561 tcgccgtggc gcgctatcac ctgggcaacc tgccgccggc gtccgcctga ccgtcgcgcg
    22621 cggccggcgg tcaccggccg cgatcctcgt ccgactcgtc agcctcgtat tcccacggcg
    22681 ggatgtcgag gctcagttcc cggtcgcgct cttcctcggc ggcgaacatc tcgcgtttct
    22741 gccgggcgaa ccgccgcttg cgccagtagg ccagcagaaa cagcaccccg gcaaagagcc
    22801 agataaagcc ggacccgctc aggaaggcga tccaggtcca gacgttgcgg tggtcgcgcc
    22861 gccactgcgc ctccagcgaa cggcggaaat ggacgtccca cagccgggtg agcagccgcg
    22921 gcccctccac cgggtcggcc agttcgcgcg ccagcggcgc cgggttgagg ccccccgggc
    22981 cggccgtcga ttccggccag cttcgctcca gatagaaccg cgtcaccgag aggctctggg
    23041 cgtaggccag ttcctgcagt tcggaaccga tcgcaacctg gctttcgagt tgctccagcg
    23101 ggatcagcgc gccgaaggcg ccggcgacct ggacgcgcca cgccgatcga taattgccct
    23161 cgccggccac cagcatggcc aggccctcgt tgagccagcc gggcagctcg ccccgcacgc
    23221 ggcgacccag aatcaggtga accatctcgt ggaccagcgt gcgctgctgc tcgacggggg
    23281 tctgccggaa gaacgcctcg cgatggatga cgatttcgtt gcggcgcgaa cgggcgaccg
    23341 cgaccacatg cgccggccgc gattgcaggt attcatggaa ttcccgctcg ctccccagcg
    23401 agacgatgcg cgccgtctga tccaggcgca gacccagccg ggcctcggcg gcctccagcg
    23461 cccgctcggc cgagtccagc agcgccggcc cccacggcgc gtccgcgtcg gcctgatgca
    23521 gacggatccg gggggtgggt gcgagggagg cgggcatggg ccgtattaga agggacagaa
    23581 aggacataag ggatataaag gacggatagg ggccattatc atccgttcag ggtacgtcgt
    23641 gaagagctca ctcaaagaca tgtcccccaa tctggagatc gcttggatcg gttctgccgt
    23701 ccctacggaa ccctatagct atgggcatcc cggttcccgc cgctgaagta gcgggctgaa
    23761 cactgccgtc cctgacggga ctgccccgcg gaagacccgg caaggccgaa ggcatttgga
    23821 tcgacggtcc acccccagtt tcctccctcg ttgtgaaccg caggatcatg agcaactcgt
    23881 agtcgctccc agaggccaat cttttcaaac cgccggtttg tgccgccggc gtttgccgtt
    23941 tcatgtgccc tgtgcttcgt gaataatgat cgccatgatc caggtccggc agctcagcaa
    24001 acgctacatg ctctacgacc ggccgctcga tcggctggcg gactggctgc tgccggcggc
    24061 gctgcaccgc ggccatccgt tctgggcgct gcgcgagatg acgttcgacg tcgccgccgg
    24121 cagttcggtc ggcatcatcg gcgtcaacgg cgcgggcaaa tcgaccctgc tcaagatcct
    24181 gaccggcacg acgcgcccca ccagcggcga ctatcgcatt caggggcgcg tctccagcct
    24241 gctcgaactg ggaaccggat tccacccgga attcaccggt cgccagaata tccagttcgg
    24301 cgccaagatg gccggcctga ccgatgccga actggccgag cggtacgacg ggatcgtcga
    24361 gttcgccgag ctgggcgact tcatcgatca gcccgtgcgc acgtattcca gcgggatgct
    24421 gctgcgcctg gggttcgccc tcgcctcctc ggtcgacccc gatgtgctga tcatcgacga
    24481 agccctctcg gtcggcgacc tgcatttcca gcagaagtgc ctgcgccgca tccgcgagtt
    24541 tcacgagcgc ggcaccaccg tcctgttcgt cagccacgat ccctcgatga tccagaattt
    24601 ctgcaacgag gcgatcctgc tggatgaggg ccgcatgatc gaccggggca aaccggacca
    24661 ggtcattgat tactacacgg cgctgctggc cgagaaatat cgcgaggggg gcgccggcgt
    24721 ccggatcgtg cggccccgcg acgacgcgcc ggcgaccgac accgcccaag ccgccgaggg
    24781 cccggcgcca gacgccgccg ccgaccagaa cggatggaag gcgcccccgc tgcacgcccg
    24841 ggggcaccgc accggcaact tccgcgcgat gatcacctcg gtctggctgg aggcgcgcgc
    24901 cgtccacggc aattcgcaga tcgtcgtcac gggcacgccg gccgtgctgc acgtccgctg
    24961 gatcgcccgc gagccgatcc cctcggccac cgtcggcatc atgctgcgcg accgcctggg
    25021 gcaggagatc ttcggcgtca attcgcacta ccgcgaggtg cggatgggac cgttccagcc
    25081 tggcgaatcg gtcgacgtcg gcttccgcat gccgatcaac ctgggcgaag ggctttattc
    25141 gatcaccgcc gccgtccatg agggggaaaa ccacatcgag acctgctacg actgggtgga
    25201 gcatgccttt tcgctccagg tgctgcccaa cccggaggag cgcttcacgg gactctgccg
    25261 cctgcccgtg gagatcgagg ttcagcgcga accgacgccg ccggccgaac gccagttcgc
    25321 cgagtgggtc cgctcgcggc ttgccagctg acgatgcggc gctacccgcg catcgtccgc
    25381 gtcctgcgct ggctgctcct gctgatcgtc gtcgccggac tgctggtcta cctgatacag
    25441 ctcgtgcgcc acggccggac gagttatcgc cacggcatgt tcgccatcat caccgggccg
    25501 taccagtggt cgtggcaggt ccatcccggg ctcagcctgc gctggctggg cgcctacgca
    25561 ctggtggcgt ggctcgtctg gggcggccgg ctgcgcacgc tggcggcgag cctgcccgcg
    25621 cgcatgctga tcatgacccc gatcctcgtg ctggccggct acctgaccgt ctcgcacgga
    25681 accggcgcgc ccgacatcca gccggcattt ctgctggcgg tcatcgtggt cgcggtcgcg
    25741 gcgtcgtatt tcatcgcgcg gctggacgac cggccggccc tgcccggccc cgccccgcgc
    25801 tgggaggcgc cgctgctcta cgggctggcg accgcggcgg gggtcggcgt cgcggttctg
    25861 tgctggcgcc tggtgtacca cggttttccg atcatgatgg acgcccagtc gcaggtcgtc
    25921 caggcgcgca tgctctggca gggacgctgg gtgttgccga tctcacaggc gctgcgcgac
    25981 gtgatcgaat tccccaattc cgttttcacc gtcccggttt attcgcaata cccgccgggc
    26041 tatattttcc tgctgatccc ggtggtggcg ctggacctgc ccccgcagac gctcaacctg
    26101 gcggcgggcg ggggcctggt ggcgctgacg gcctggctgg cgcggcggct cggcggcgcg
    26161 cgggccggct gggcggccgc cgtcctctgc gccggctcgc cgttcatgat cctgtgcggc
    26221 acggcgatga accacgccac cagcgctctg gcactgctgg gaacgacctg ctgcctgctc
    26281 ccccggctgg gccgcgccga gccgcgacgc tgggcgctgc gcggcctggt cggcggcctg
    26341 ctcctcggct gggccgtatc gacccgaccc ctgaccggat tggcgcacgc gctcgtctgg
    26401 ctggcggcgt ggctggcgca actgggcgaa ctggcgtggc gccgccgctc cggccggatc
    26461 gtccgccccg ggcagaccct ggccgccgtc ctctggcgcg gcctgtgggt gctgctcggg
    26521 cttatcccgc ccgcgctgct gttcatgttc tacaacctga agacgaccgg cagcccgttc
    26581 atcttcgcct acgaggtcag caatcccgac ctgcatcgga tcggtttcgg ccaggccacc
    26641 gaactccatt acaccccggc cgacgcgctc cacaactcca tggcgagcta tctggacctc
    26701 aatatcaagc tgttcggctg ggcggtgggc tcctggacgc tgatcctggc gtggtggctg
    26761 cgcacgcgcc tgacccgggc ggagctgacg ctcctcgggt tgatcgtgtc ccaatcgatc
    26821 ttctacggcc tgtatcagta tcacgacctg attctggggc cgcggttcct tttcgatctg
    26881 ctgccgtatt tcgcggtgct ggcggcgctc gggctggctc ccgcactgcg tcgcgggcga
    26941 cgccggggga tggtgctggc gctgatcgtc ttcgcatttt cgatcgtcgg cattcagcgc
    27001 ggcatcaatt tctggatcac caaattccgg ccggtgatcg agcgcaacgt ccagttcgcc
    27061 gggttcatgg acgagatcga accgctcgag gggcccacgg tcgtcgtcat ggaccagccc
    27121 tacaacgagg tcatcggcca gtacttccag cacccgcgct atcaacaccc gctctggttc
    27181 gtcttcgagc atcgcgaggc cgaagcgcgc ctgcttccgg aactggccgg gttccactgg
    27241 gtccgatttg atgacggcaa ccgtccggcg ccgggcccgc ccccggaact tccgcagttc
    27301 cctcagggcg gcgaaccggc aagttgaacc agagccgttc ggcatgggac cggcgcgctc
    27361 gccagtcatc cgcccgccgc ttcggatccc ccaccgccgg gttgataatc cgggatcagt
    27421 tccttgagca tccggcggat cgcggcgtcg tcccgcagca cggccagcgc ctccagctcg
    27481 aggatccgcg gtcgcaattg ctcccagtcg tgcgccggct gcgacgagcg gaaaatcttc
    27541 ggatgcgccg tcggctcgcg gccctgctcg gccgtcaaca gctcctcgaa cagcttctcg
    27601 ccgggccgcg cccccgtgaa gacgagcggg atatcgcgct cgggctccag cccgctcagc
    27661 gtgatcaggt tgcgcgccag atcgacgatc ttgaccggct cgcccatatc gagcaggaag
    27721 acctcgcccc cctggccctg cgagccggcc tcgatcacca ggctcaccgc ctcggggatc
    27781 gtcatgaagt agcgcgtcat ctccgggtgg gtcagcgtca ccggcccccc cgcctggatc
    27841 tgccggcgga agaggggaat caccgaaccg cggctgccca ggacgttgcc gaaccgcacg
    27901 gcgacgaagc gcgtcgcccc cgggcggttc agcgcgctca cgatcatctc ggccaggcgc
    27961 ttgctggcgc ccatcacgcc ggtcggccgc acggccttgt cggtcgaaat caggatgaac
    28021 cgctcgaccc cggcggcccg cgccgcctcg gccaggacgc gcgtccccat gacgttgttc
    28081 ttgatcgcct cggccggctg cgcctccatc agatggacgt gcttgtgcgc cgccgcgtgc
    28141 aggatcacgg cgggcctggc ttcggccagc agcggctcga tcagcgccgc gtcgcggacg
    28201 tcgcccacga ccaggcgcag cgcggcgccc cgctcccggg ccggcgccga cagctcctgg
    28261 tggatgtcat agaggctgtt ctcgccgcgc ccgagcagaa tcagctcgct ggccccatgc
    28321 gcgagcgcct ggcgcgccag ttcgctgccg atcgacccgc cggcccccgt gatcagcacg
    28381 cgccggcccg tcaggcacga ttcggccggc cccgccccgc gcacgaccac cggcggccgg
    28441 cccagcaggt cctcgatctc caccggccgc aggcggctga cctcgacctg gccgtggacc
    28501 acctcctcca tattgggcac ggtgcggaac gccagccgcg cctcgcggca gtggtcgacc
    28561 accgaccgca tcaacgccgg cgtcgcgcgc ggcagc
//
LOCUS       DAOPCO010000022        28543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1803_length_28543_cov_5.653924, whole genome shotgun sequence.
ACCESSION   DAOPCO010000022 DAOPCO010000000
VERSION     DAOPCO010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28543
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1803_length_28543_cov_5.653924"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /gene="coaBC"
                     /locus_tag="PLS90_03185"
     CDS             <1..1075
                     /gene="coaBC"
                     /locus_tag="PLS90_03185"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPK01441.1"
                     /translation="LTFESLCGHPVYSDVFARPSAWEMEHIAWARWADALLIAPATAN
                     VIAKLAHGLADDAVTTLALAFTGPVWVAPAMNTAMLNHRATRHNLELLAARGVRVIEP
                     GAGLLACGEVGPGRLAEPEELIAALARGLGGERAAGSLAGRRILITAGPTREPLDPIR
                     FISNRSTGRMGAELAAEALARGAQVRLIHGPLAVAIPPGCDAVPAETARAMLDAVQQA
                     WPETDIAIFAAAVANYEGARVAGGKLKGGETLTLALRRTPDIAAWAGANRRPGQVLVG
                     FAAESEDLIAAGRRKLQAKRLDLICANPIGESGIGFTAEQNRVTLLTGDEAAEPVTSP
                     TADKRAIAAWILDHVEQRLAAAT"
     gene            1225..1914
                     /locus_tag="PLS90_03190"
     CDS             1225..1914
                     /locus_tag="PLS90_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01442.1"
                     /translation="MSRLSVRPLTTWGRWGLLGFLCLTPLLPAQPGKVKVRRLDLEPL
                     SPARVDQLNEAAREAYQQGMVALDRVNYPLALEFFQRAVREEPDNIYLRFQVVQVAHY
                     LGDTRSVDKHAIRYYDIAEDNLRAMTESDALNEREKGRAAEAIEMVQQLRRSASERDE
                     KRRTAGRELAEEIRKELYLDLKEKEKDLRQQREEMIGERRRMEELRQTQGSRGGSSRG
                     GMMGMPGMAPF"
     gene            2013..2351
                     /locus_tag="PLS90_03195"
     CDS             2013..2351
                     /locus_tag="PLS90_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPK01443.1"
                     /translation="MKLIVAIIKPFKLTEVKEALNEIGVTRMTVSEVKGYGRQKGHSE
                     LYRGTEYQIDFVPKIRLEIAIDDSDLDQTVETLTRAAQTDSIGDGKIFVLPLEEAYRI
                     RTGEQGPDAL"
     gene            2510..4243
                     /gene="polX"
                     /locus_tag="PLS90_03200"
     CDS             2510..4243
                     /gene="polX"
                     /locus_tag="PLS90_03200"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HPK01444.1"
                     /translation="MTQDKQDLVAALKRCAILMELAGENAFKCRAYEQGARIVESLEG
                     EPRQWLETGRLNGVKGIGKGLLKAIEEWAASGRICELDELQEKIPPGLLDILHVPGIG
                     PKKIKLLWETRGIDSLERLEQAARAGELNDLAGFGAKTVEKMLAGIEQRRQYAERHLI
                     HTATAAAERILGHLRAVPGIGRLELAGSLRRRRETIKDIDLVATAADPAAVMQAFVTM
                     PGVLRVVGHGATKSSILLAGGIPADLRVVAEDEFAAALNYFTGGKEHNTALRGRARRM
                     GLKLNEYGLFREAPGDEPGERLPTPDEAAIYQRLGLAYIEPELREGEGEIEAAEAGEL
                     PRLVTADQVRGVLHCHTTYSDGRLGVLELAAACRDLGYEYLCICDHSQSAAYAGGLRV
                     DAIRRQWDEIDAANAQLDGFRIFKGIESDILAEGELDYDDGLLARFDLVVASIHSRMT
                     LDREAMTRRICRALAHPAVRILGHPTGRLLLRREPYGVDIDQVIETAVRHNVLLEINA
                     HPHRLDLDWRLVRRAKARGARFAVNPDAHRREELGYIPYGVGIARKGWLEAEDVINML
                     PLKQFEQWLKR"
     gene            complement(4374..4733)
                     /locus_tag="PLS90_03205"
     CDS             complement(4374..4733)
                     /locus_tag="PLS90_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01445.1"
                     /translation="MAGEQEPRDVNRVAPEAPITVSCRLIEGDWHCTALQFDILGVGN
                     TREAAFAQLRELVEAYLRHALASKHEVKLFNPAHREEWNNPGREFFDLAIHVRSARTS
                     GRPSRLRLQELPTVGGR"
     gene            4934..6523
                     /gene="purH"
                     /locus_tag="PLS90_03210"
     CDS             4934..6523
                     /gene="purH"
                     /locus_tag="PLS90_03210"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPK01446.1"
                     /translation="MAKRRAVVSVSDKRGIVEFCRGLVAADFEIISTGGTLKALTAAG
                     VPARNVSEYTGVPEILGGRVKTLHPMIFGGILARRNLADDLSTLAEHGMQTIDVVAVN
                     LYPFAETVANPRATREEIIEQIDIGGPSLIRAAAKNHGDVYVVVHPEDYDRVLDAVRS
                     GREADHIALRRRLAAKVFEHTAHYDRLISDYMRQAFAEPGDDAHAAGPEQAPAAALPD
                     RLTLGWPKRQALRYGENPHQAAAFYADPDCPPPSMAHCRQLQGKELSYNNYLDGETAL
                     EMIREFEDPACVILKHLNPCGAAWGENCVDAYRDALACDPTSAFGGIVGVNREVDRAL
                     AEELGQIFLEVIIAPSFSAEAREVLAAKKNLRLLATGELTPRATRLAYKSVSGGMLVQ
                     QTDTGRLNDKELKVVTDKQPNDEDWRALRFAWKVCKWVKSNAIVVTDARRTRGIGAGQ
                     MSRIDATNFCLQKAGGKVEGGYLASDAFFPFRDVVDLAHAAGIKAIIQPGGSIRDNES
                     IQAANEHGIAMVFTGMRHFRH"
     gene            6520..7335
                     /locus_tag="PLS90_03215"
     CDS             6520..7335
                     /locus_tag="PLS90_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK01447.1"
                     /translation="MTPGPHDPWLFFLIAGLCVVLSGIAKGGFGGGVGVIATPLLSLV
                     VSPLVAAGILLPLLCACDLFAVWHYRRTFDRGNLVQLVPGGLVGILIGAGLLWHLKGE
                     TAAAERALRILIGVIAILFVIWQAGRAWFLKHLPPARPGTVFGAAMGVTAGFTSTLAH
                     AGGPPVTIHLLPQRLPRQVFVGTTVWFFTIANYVKLAPYAALGMLDLANLRISAMLLP
                     LVPVGVYAGIWCNRRINDKWFNIVVYTLLLLVGIQLLSGYDPLSLLARITSLR"
     gene            complement(7518..9185)
                     /gene="bshC"
                     /locus_tag="PLS90_03220"
     CDS             complement(7518..9185)
                     /gene="bshC"
                     /locus_tag="PLS90_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HPK01448.1"
                     /translation="MAVRIQQSVWDQLKAREPAPGQRWLRWHPSELEDCLAQRAGHSP
                     SPLAEHELAELETAHRRWGADEATLAAIRELGTPRARVVVTGQQPALLAGPLLVLYKT
                     LAAIRLAGRLRERHPDLTFVPVFWVASEDHDFDEIRRAYWPGHEGGLEEFLLAAETTP
                     GRMVGGLRIGEQLARLAHQIEMSTLTTEFRDGVLARLRGGDGAEVTLEDDFCRLMLEL
                     FRGTGLVIVSPLMEWVRRRAAPIVQTELARPGESTARILERTRALEAARFEAQLHRAP
                     DSLNVFWIDGARRRLALRLDGAERVRPVAPGGGDEAPEDVALDELRRQVERAPERFST
                     NVVTRPLVQDSALPTVAQVVGPGEAAYLAQVEAVYESFGVFTPVRWPRPSVTLLEPRV
                     ERRLEKHALALEQALTRDADELVEMILQRDLGQGQIGRIESLRARQKEELEALRREID
                     ASAAAGSAIDKLQQAMDKGYDKLLDRVLTQHKQDRGHLMRAMALIANSLHPARLAQER
                     ALNPIVPFAINYGMGWAVRLMARVELDYWAEPQVISLAEELSGAAER"
     gene            9369..10256
                     /locus_tag="PLS90_03225"
     CDS             9369..10256
                     /locus_tag="PLS90_03225"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPK01449.1"
                     /translation="MPPSAKRRPVAAAAPKATVFNTSQLSRFDRERRRRLGVELLGGV
                     DEAGRGALAGPVVSACVVLEPGTRLGGVNDSKVLTPEDRERLVPQIMERAAAWSVGWA
                     SAAEIDRINILEATLLAADRALRALGDRTPQHMITDFLKLRWAPCPVEPIVDGDARSL
                     AVAAASILAKVARDRIMTALCAEYPCYGFSRHKGYGTRAHWRALDSHGPSTLHRLTYN
                     GVCFFNASPDVRSRRRRCLESIPSEPQPPDWLEVLTSPPDQLDPLPFLPEAEWELSVS
                     YASETSLVPRMKIRVSEGT"
     assembly_gap    10496..10595
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10740..11366
                     /gene="ruvA"
                     /locus_tag="PLS90_03230"
     CDS             10740..11366
                     /gene="ruvA"
                     /locus_tag="PLS90_03230"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPK01450.1"
                     /translation="MIEYLRGILLERQADHVVVDVGGVGYGVAVPASTAQTLGDAGSQ
                     VALWVRTYVREDILRLYGFKTRHERDVFDIFLGLSGVGPGTGLALLSSLTVAQIIQAA
                     AAGDAARFKKVKGIGQKMAEKLVLELKGRVDRLVAGLPEELAREATAEAAPLSDNARD
                     AVAALEALDVRPQQARRAVVLALEALGEDASVQQLVREGLKHRRATAP"
     gene            11427..12791
                     /locus_tag="PLS90_03235"
     CDS             11427..12791
                     /locus_tag="PLS90_03235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK01451.1"
                     /translation="MPSLPAMLGFAMPNAISFQIALGPPLVLMARHWGASSFYIGAIA
                     ALVPLLTMTQLYMAPRVEYLGFKRVMLAGWTGRIVTLILTAALPFCSGFLSRRFQLEA
                     LFAFMFLFNLLRGLATASWMPWVKELVPERWRGRYLSAEQVVVNISSALTLFGCGWVL
                     GDDPGDFQFGFLFIVSFVAGMTSLYFLKRIDCPPPLEGAPTLEPVRRWVNRVWRVKPF
                     RRLIRLNMLWTFALGAWGTFTVVYLRDGLHFPERIILYMSASSTLGALLSAWVWGLMA
                     DRFGSRPVMALTSRLILLTALCWLLLTLGLIPPAVGTVLILYILLGMGTSGFGVAINQ
                     YSLNNAPAGFPVLALSLFAATSSFSSALSPFLWGALLDLLAPVRPRVGPLLLHQYVWF
                     YALIALMIVASKHLIRQLPDHKAAATHIVLYHMITDYPLRTLYAVQGMLTGRTRREGP
                     PTRG"
     gene            complement(13009..14538)
                     /gene="glpK"
                     /locus_tag="PLS90_03240"
     CDS             complement(13009..14538)
                     /gene="glpK"
                     /locus_tag="PLS90_03240"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPK01452.1"
                     /translation="MGSYIGAIDQGTTSTRFMIFDEEGREVARHQLEHAQIMPQPGWV
                     EHDAREILRNTEAAIAGAMARAGLRASDLAAIGVTNQRETTVAWYPRTGEPCGNAIVW
                     QDTRTEELMRAHRERGEEERVRERTGLPLSTYFAAGKMEWMLTEHPELRRAAARGAAV
                     FGTIDSWVIWNLTGGADGGAHVTDPSNASRTLLMDLRAGAWDPELADLFGVPMEMLPA
                     IRPSSDPQAYGLTRAGGPLGGEAPVCGALGDQQAATMGQVCFDPGEGKNTYGTGNFLL
                     VNTGTERIRSTAGLLTTALYRLGAGGLTYALEGPIAVTGSAVQWLRDQLGVIRDAGEI
                     EALAASVPDAGGIYFVPAFSGLFAPHWRPDARGAIVGLSRFHTRAHLARATLEAICYQ
                     TRDIVEAMVRDSGRPLEVLRVDGGASVNNLLMQMQADILGTPVVRPKVTETTALGAAY
                     AAGLAAGAWRGLADLRRHWQADAEWMPRWSADQREAGYAGWRRALKRTLGWSTSQENP
                     E"
     gene            14729..17047
                     /locus_tag="PLS90_03245"
     CDS             14729..17047
                     /locus_tag="PLS90_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01453.1"
                     /translation="MSPLASALPPDRRPRRRRARSPRARRLLALFLLALAGRAPAQVE
                     IDGDFSDWTSIPAMTCADSSAHDGELGRVAQWKVAADPQFLFALIQFEAPRPLVNPAG
                     STELQPGRWDDFGYLDLDINRDGQWDYRAQLSEGKRPGLNNLVVMNLDADGNPRGRLL
                     YPEGHKNYFPLGPRARLSADGRTIELRIPRRPLRLDVPATIDLRVRTRYRDSVRGGGG
                     WVTEFTPGAAGCAFQLGGRLRPTDDPVLTPGAVNLPPARRAEGAEELEIAGSHERLAP
                     SARPFLIPNLPQESVRTAPPIDPTPLPEAAPLSDEAAAAARSMSEAMTAVEQARQAGI
                     EEALAVAVTPTPVPTATPTPTPDPVAVQAEAFDQEVDSLISSIGTEPGQPAAAPAARP
                     EVPAPGMELVPPIVAPGAEVAPPAPEAELEAPSPEELQRTFDQNIESLIDAIQSEGGA
                     ATGAVRSPVAVGPTPAPEPSPTPTPEPTSTPTPEPTLTPTPAPTVSPTPIPTPDFTPR
                     PTPGPRPTATPLPTATPPPESTPTPEPTPAPAPEPTAEPTPTPTPSPVLTPTPTPTPE
                     PRGLNIADVPFAQPGDEPITLDADPSDWEHADYVHVPSADARSTAAHPIALVDRILVA
                     SSSGFLYTRIDFAESRFALLPPADGWPIDAGAYLAMDVDLDGDWDFEVQALRGAAGFV
                     PAQVVNVDGAVVLAAQEDEGARPDGPIARFDEEGRSLELRLPRAPLWLDGDVRLRMRV
                     LVRYQLEPEAAWRQMQYPDVKDGWFAFPLRKE"
     gene            complement(17052..18536)
                     /locus_tag="PLS90_03250"
     CDS             complement(17052..18536)
                     /locus_tag="PLS90_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK01454.1"
                     /translation="MPKPRRTLAAHSQPSLPGLNTGLDLTTYKTIRLAVSGDVELTRL
                     ETRLIDTPAFQRLRFIRQLGAAYLVYPTALHTRFDHSLGTLGAARRMIEAIRGNARAH
                     SEERDIPPEEELLARLFALLHDIGHIPFGHTLEDELGLFARHDASRERIERFVGPESA
                     AGRLLRPALGGELYQRLLRLFEAAAGQCEPAADDVYILDLVTNTICADLLDYLARDAF
                     FCNLSVEAGDRFYRYLALRRHGGRRRLVVQLWKENKDAPRRDVLGELIRLLDNRYVLS
                     ERVYFHHAKLVAGTMVGAAVARALAAGTLDPAELFDLGDDTLLWRLARGEPPQAAGLA
                     RAVLERRLWKLAARWDREALGAEGVAGTVAGPPHAADGEDRAPAVEHLLARLHADAAR
                     RRRLEERRAAELGLDPGELLIHCPPLKMAMKLADTMVLWRGAIRPLRDCRDDALTGIK
                     LQAILDSHRNLWATRVFVPPERLAAREALARLTPPERRRWLGGE"
     gene            18660..19934
                     /locus_tag="PLS90_03255"
     CDS             18660..19934
                     /locus_tag="PLS90_03255"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPK01455.1"
                     /translation="MNEVFTPIPQAIEAIRKGEIIILIDDEDRENEGDFVFAAEHATP
                     EKINLLATHGRGLICVPAARERLAHFLLEPYPENQNTALMGTNFTVSVDARHGVTTGI
                     SAPDRCRTVRVLADPRSRPSDLVRPGHIHPIAAKDGGVLTRAGHTEATVDLCRLAGLE
                     PVGVLCEILKDDGEMARLPDLIQIARRFSMPIVTIRDLIGYRQRTEKLVKRAVTTRIP
                     NAYGEWELTLYEDLVNDELHLAMTMGEINDEPLLVRMHSQCFTGDTLGSLRCECGPQL
                     FTAMDAISRFGRGVIVYLHQEGRGIGLKNKLLAYALQDQGLDTIEANEKLGFKADLRE
                     YGIGAQILVDLGVRRIRLMTNNPRKIVGLEAFNLEIVERVPLEVGHCEFNQHYLETKR
                     RRLGHMLSGAAVLGAAPADATDDGDSATKQVS"
     gene            19987..20472
                     /gene="ribE"
                     /locus_tag="PLS90_03260"
     CDS             19987..20472
                     /gene="ribE"
                     /locus_tag="PLS90_03260"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPK01456.1"
                     /translation="MQVLEGDYDARGLRLALVASRFNEFIVGKLVEGALDAFVRHGGD
                     PGAVTLAWVPGSLELPPVLARLAQSGRFDAVIALGCVIRGGTDHYTYVAGEAAKGVAQ
                     VALNSAVPVIFGVLTVDSIEQAIERAGTKMGNKGADAAVAAIEMARLYARLPGAKTGK
                     A"
     gene            20472..21344
                     /locus_tag="PLS90_03265"
     CDS             20472..21344
                     /locus_tag="PLS90_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01457.1"
                     /translation="MPIHGGAFPPPPEAVRAARRTLRRQAPPLIARFLNHLTACPAIA
                     PDRVDTAWALAALAREMAGALAAAQQTGGRPVVELVFALHRRLGHGVATPAPAPTIDL
                     APVLRGLAPYEAWLARLTADSPPWRAALMRWIDVLSERLTMHRYPDPYRRRQHAHALL
                     GRPLELLAAPAVGAAEIDPVALALLRFWDLARARAYAGRGWNRLDSPADRLGGFYGLA
                     DEARPPTPEEIRSAAAALHAELNGEWPAWAEPLRADLQAATASYLESNRWPAWTTADR
                     PAGLIQRMFGRAQG"
     gene            complement(21423..21761)
                     /locus_tag="PLS90_03270"
     CDS             complement(21423..21761)
                     /locus_tag="PLS90_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPK01458.1"
                     /translation="MVREMNGIALCIGGTEDHVHIYAKMHQDFSVSTMLRTIKSKSSG
                     WVHRTIPELGEFQWQNGYACFTVSQSGDAKLACYIQRQEIHHHARSFRDELIALLKAH
                     RVEFREAFLQ"
     gene            complement(22030..24816)
                     /locus_tag="PLS90_03275"
     CDS             complement(22030..24816)
                     /locus_tag="PLS90_03275"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPK01459.1"
                     /translation="MALATPKEPFRAIATTPGELDRLITAACREQMAAVIAGNGGGEV
                     FFIGRLNPARVVEAVEPLAFGNRGAVPALLPYARPGDVCIHNHPSGRLEPSEADIAVA
                     SELGGRGVGSYIINNDCTALRIIVRALEPPTLQPISVGKLANWLKPGGRLAERLAGFE
                     YRPQQIEMLQAVGEAFNSDGIALIEAGTGTGKSMAYLLPAIAWAIRNNEKVVIATGTI
                     NLQEQLIEKDIPLLLQNSGLQFEATLMKGRNNYLCRRKAQYLREHPDFLQIGEKAAQL
                     AEIQAWIRTTRTGSLEDLPFTPDPEVWESVMSEADNCLRTRCPFYDTCFFYTARRRAA
                     RAQVLVVNHHLLMADMAIRAETENYSATAVLPPYQRLIVDEAHNLEEVATSYFGVRVS
                     RGSLAYCMRRLFNTRRNDGLLIWLAGRIHNGEYPVAPQQHDELLLKLGRDLPLQHHEL
                     LTAMEEATGRLAEALERTERTPLHQPLEIKRRIKTEDLAGPFWREEIDAPLRAVVTAA
                     RPYLENLRAVGRALLPFLEEASPELATPLLELQSSLNKVEAAIGRLIKFLGSAEGQCR
                     WIEYRRRPGGRLPDVTYCLAPLNMAAELRENLLRRCRTVVMTSATLTVDQRFDYLLRQ
                     IGAEDPLQLGLIGPTAAEASASAAPGGEELEPPPAEPFAENAPSAAEIIELIPPLPSD
                     ASAEPDELPPPRARPFRALILATPFDYERQVYVGVPMDMAEPTDPRFEEALTGFLGPT
                     LEISRGRAFVLFTSYALLGRVFDRLAPGLEARGYPCLRQGTIGRSMLTEAFRNEIGSI
                     LFATSSFWEGVDVAGEALSCLVLTRLPFKVPGDPLTEARIEDLRRRRLDPFYHLVIPQ
                     AVIKFRQGFGRLIRTKTDRGAVLICDPRVATRRYGQIFLRSLPTQRIHFARQSELLDA
                     LRTFYDDQPGRDDD"
     gene            25393..25484
                     /locus_tag="PLS90_03280"
     tRNA            25393..25484
                     /locus_tag="PLS90_03280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25427..25429,aa:Ser,seq:gct)
     gene            25717..27357
                     /locus_tag="PLS90_03285"
     CDS             25717..27357
                     /locus_tag="PLS90_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK01460.1"
                     /translation="MPVAMVLLCLIALILPTPSAWAQPAAPEAPPDEPEWTLLAPLRV
                     VRLDNGMTFLLYPNDRAPIFSGVIRYDVGGKDEIPTQTGISHMFEHMAFKGTSRLGTT
                     DYAAEQEALAEVERLAYAYDRLRNQLARDDSPTTARLDALRELAMQFQAAQAAAGQYV
                     VKDEFDEIYSREGGSQMNATTSPDATTYFISLPANRLPLWARMESERLADPVPREFYR
                     EREVILEERRMRLDNSPLGRLWEITMATAYVAHPYGYPTIGWESDIANLKATEAMAFY
                     RRHYTPDRAVGVLVGQFNPEEAEALLRETFGKLPPAPEDLAEHRIVPEPEQMGERRAT
                     LEFPATPALLLAWHKPPAPGKADVAAELLGQIVAGGRSARWFERLVKEERLAAEVHTF
                     TGPGDREPNLFMIYATPQPGHTLDELEAALREEVRRVRTEPVSAEELERARKNLRADT
                     IRALQTNLGLARTLAEYAQIGAPAPGEIDPWYLERRLHQLEGLTAGDLLAFAAEYLTD
                     ANLTVGSLTPPPSAEETPPGAGARGGTDSGASEETQGT"
     gene            complement(27464..28390)
                     /locus_tag="PLS90_03290"
     CDS             complement(27464..28390)
                     /locus_tag="PLS90_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01461.1"
                     /translation="MFVTISPPAWSGRPATPTPTASPTPEPVVVQTPEPTPEPTPEPT
                     PSPTPAATPVIEPTDWLIEPSADYEIPIETESWPVTDAPVAETLAPDEAFEEPAEESP
                     PDDAAAAAAQPEPADEERPDPSVPVPQLDLPEELRKGRSRQIDRRLAMLQSKLSADQT
                     LFRTEGLNEGAIREIDITGVPPNVSQEVLGRYGIRIMLMNLNGNDPNPGFLSRAQTNE
                     GQFVKRSGSGVFQVFNYGPPAIARMMQLEEAELEKQGLDPKTSRVVRVVFGVVRTTKG
                     YDLGIKKLEAEPVPGLADEEETPPPAPPTGGG"
     gene            complement(28353..28535)
                     /locus_tag="PLS90_03295"
                     /pseudo
     CDS             complement(28353..28535)
                     /locus_tag="PLS90_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
BASE COUNT         4436 a        10119 c         9390 g         4498 t
ORIGIN      
        1 cctcacgttc gagtcgctct gcggccatcc ggtctacagc gacgtcttcg cgcggccctc
       61 cgcctgggaa atggagcaca tcgcctgggc gcgctgggcc gacgccctgc tgatcgcccc
      121 ggccacggcc aacgtcatcg ccaaactggc ccatggcctg gccgacgacg ccgtcaccac
      181 gctggccctc gcgttcacgg ggccggtctg ggtcgcgccg gcgatgaata ccgccatgct
      241 gaaccatcgc gccacacgcc acaatctgga gctgctggcg gcgcgcggcg tccgcgtgat
      301 tgagcccggc gcgggcctgc tggcctgcgg cgaggtcggc cccggccgcc ttgccgagcc
      361 cgaggagctg atcgccgccc tggcgcgcgg cctggggggc gagcgcgcgg ccggatcgct
      421 cgccggccgc cgcatcctca tcaccgccgg accgacgcgc gagccgctcg atcccatccg
      481 cttcatcagc aatcgctcca ccggccgcat gggcgcggag ctggccgccg aggccctcgc
      541 gcgcggcgcc caggtccggc tgattcatgg gccgctcgcg gtggcaattc ccccgggctg
      601 cgacgccgtg ccggccgaaa ccgcgcgcgc catgctcgac gccgtccagc aggcctggcc
      661 cgagaccgac atcgcaatct tcgccgccgc cgtcgccaat tacgaaggcg cccgcgtcgc
      721 cgggggcaaa ctcaaggggg gcgagacgct gacgctcgcg ctgcgccgca cgcccgacat
      781 cgccgcctgg gccggggcca atcgccggcc cggccaggtg ctcgtcggct tcgccgccga
      841 gagcgaggac ctgatcgcgg ccggccggcg caagctccag gccaagaggc tcgatctaat
      901 ctgcgccaac ccgatcggcg agtcgggcat cggctttacc gccgagcaaa accgcgtgac
      961 cttgctgacc ggggacgagg ccgccgagcc cgtcacctcg cccaccgccg acaagcgcgc
     1021 gatcgccgcc tggatcctgg accacgtcga gcaacgcctc gccgccgcga cctgatcctg
     1081 ctctacaccg atttccatcg ctcgttgatc ccgatagagg ttgactcaac tttcgcttgc
     1141 cattcggcaa gcgtgttctg agaatggagc accgtgcccg ccgcgggcgc gggcgatccc
     1201 gcatgccatt gagggaggaa tgccatgtcc aggttgtcgg tccgtccatt gacgacatgg
     1261 ggacgatggg ggctgctggg cttcctctgc ctgacaccgc tcctcccggc tcagcccggc
     1321 aaggtgaaag tccgccggct cgatctggag cccctctcgc ccgcccgtgt ggaccagctc
     1381 aacgaggcgg cccgcgaagc ctaccagcag gggatggtcg cgctggatcg cgtcaattac
     1441 ccgctggcgc tggaattctt ccagcgggcc gtgcgcgagg aacccgacaa catctatctg
     1501 cggtttcagg tggtccaggt ggcgcactac ctgggcgata cgcggtcggt cgataagcat
     1561 gcgatccgct actacgacat cgcggaggac aacctgcgcg cgatgaccga atccgatgcg
     1621 ctcaacgagc gcgagaaggg ccgcgcggcg gaggccatcg aaatggtcca gcaactgcgc
     1681 cgcagcgcct cggagcgcga tgagaaacgc cgcaccgccg gtcgcgagct ggccgaggag
     1741 atccgcaagg agctctacct ggacctcaag gagaaggaaa aggatctgcg ccagcaacgc
     1801 gaggaaatga tcggcgagcg ccgccgcatg gaggagttgc gccagaccca gggctcgcgc
     1861 ggcggctcca gccggggcgg gatgatgggc atgcccggca tggccccctt ctgacgcgct
     1921 ccgtcagcat gtgcacgtga gattcgatcc gggggaggcc tgcgagggcg ctcccccgct
     1981 atcatgatag gagcagggtg ttcccgcgca ggatgaagct gatcgtcgcg atcatcaaac
     2041 cgttcaagct gaccgaagtc aaggaagccc tcaatgaaat cggcgtgacg cgcatgaccg
     2101 tctcggaggt caagggctac ggtcgccaga agggtcacag cgagctttat cgcggcaccg
     2161 aataccagat tgatttcgtc cccaagatcc gcctggagat cgccatcgac gacagcgatc
     2221 tggatcagac cgtcgaaacg ctgacccgcg ccgcgcagac cgactcgatc ggcgacggca
     2281 agatcttcgt cctgccgctg gaggaagcct atcgcatccg caccggcgag cagggccccg
     2341 acgcactgta agcgttcgtt agaattcagc ggacctccct tcgtctctcg cctctcgtcg
     2401 ctcgtcgctc gtcgctcgtg attgaagact tgatgtcgaa tgcgaatttg ccggttttcg
     2461 agagccgagg acgagggccg agggacgaca agcagaccga agctcaccga tgactcaaga
     2521 caaacaagat ctcgtcgccg cgctgaagcg ttgcgcgatc ctgatggaac tggccggcga
     2581 gaacgccttc aaatgccgtg cctatgaaca gggcgcgcgc atcgtcgaaa gcctcgaggg
     2641 cgagccgcgc cagtggctcg aaaccggccg tctcaacgga gtgaagggga tcggcaaggg
     2701 cctgctcaag gccatcgagg agtgggccgc cagcggccgc atctgcgaac tcgatgaatt
     2761 gcaggagaag attccccccg gcctgctcga catcctgcac gtccccggca tcgggccgaa
     2821 aaagatcaag ctcctgtggg aaacccgggg gatcgacagc ctggaacggc tggagcaggc
     2881 ggcgcgcgcg ggcgaactga acgacctggc cggcttcggc gccaagaccg tcgagaagat
     2941 gctggcgggc atcgagcagc gccgccagta tgccgaacgc cacctgatcc acactgccac
     3001 cgccgccgcc gagcggatcc tcggccacct gcgcgccgtg ccgggcatcg gccggctgga
     3061 gctggccggc agcctgcgcc gccgccgcga gacgatcaag gacatcgacc tggtcgccac
     3121 cgcggccgat ccggccgccg tcatgcaggc cttcgtcacg atgcccgggg tcctgcgcgt
     3181 cgtcggccat ggcgcaacga aaagctcgat tctgctggcc ggcggcatcc ccgccgacct
     3241 gcgcgtcgtg gccgaggatg aattcgccgc cgcgctgaac tacttcaccg gcggcaagga
     3301 gcacaatacc gcgctgcgcg gccgcgcgcg ccgcatgggc ctcaagctga acgaatacgg
     3361 cctgttccgc gaggcgcccg gcgatgaacc gggcgagcgc ctgccgacgc ccgatgaagc
     3421 cgcgatctac cagcgcctgg ggctcgccta tatcgagccg gagctgcgcg agggggaggg
     3481 cgaaatcgaa gccgccgagg cgggcgagct gccgcggctc gtcaccgccg accaagtgcg
     3541 cggcgtgctg cactgccaca cgacctacag cgacgggcgc ctcggcgtgc tggagctcgc
     3601 cgccgcctgc cgcgacctcg gctacgaata cctctgcatc tgcgatcaca gccagtcggc
     3661 cgcctacgcc ggcggcctgc gcgtcgacgc gatccgccgc cagtgggacg agatcgacgc
     3721 cgccaacgcc cagctcgacg gcttccgcat cttcaagggg atcgaatccg acattctggc
     3781 cgagggcgag ctggactacg acgacgggct gctcgcgcgg ttcgacctgg tcgtcgcgtc
     3841 gatccacagc cgcatgacgc tggaccgcga ggcgatgacg cggcgcatct gccgcgcgct
     3901 ggcgcacccc gccgtccgca tcctgggcca cccaaccggg cgcctgctgc tgcggcgcga
     3961 accgtatggc gtcgacatcg accaggtgat cgaaaccgcc gtgcggcaca acgtcctgct
     4021 ggagatcaat gcccatccgc accggctcga cctggactgg cggctggtgc ggcgggcgaa
     4081 ggcgcgcggg gcgcggtttg ccgtcaatcc cgacgcccac cgccgcgagg aactgggtta
     4141 tatcccctac ggcgtgggga ttgcgcgcaa ggggtggctg gaggcggagg acgtcatcaa
     4201 catgctgccg ctgaagcaat tcgagcagtg gctgaaacgt tgacgcggtg ttacaccggg
     4261 gtatgcagcg ttcgcaaccg ctggtgcggg gtcatgcgaa gatcgtcgct gaagccatcc
     4321 ttggcggtca accgcaacgc cttgcgcgct ttcgcgagat acaggtgacg aacttatcgt
     4381 ccgcctacgg tcggcagttc ctgcagacgc agccgcgacg gccttccgga agtcctcgcc
     4441 gaccggacat gaatcgccag atcgaagaat tcccggccgg ggttgttcca ttcctcccga
     4501 tgggccggat tgaacagctt gacttcatgc ttcgaggcga gggcatggcg caggtaggct
     4561 tcgaccagct cgcgcaattg agcgaacgcc gcctcccgcg tgttgcctac gccgagaatg
     4621 tcaaactgca aggcggtgca gtgccaatcc ccctcgatca gccggcagga cacggtgatc
     4681 ggtgcctcag gcgcgactcg attgacgtct cggggttctt gctcgccggc catcttcccc
     4741 aacctgccct gtcgtctcca aaccgtctta tcaattgaac cagaataatt caccaccccg
     4801 cggaaagctt gaaaaaaagg cgctcgcccg ccatcctgtg ggcgggctca tggccggttc
     4861 tccccgcgcg gcgcgccttt cccctctcac cggcgcgggc gcatgagtta tctcctggat
     4921 gaggcgggct gtcatggcga agcgacgggc ggtcgtcagt gtttccgaca aacgcgggat
     4981 cgtggaattc tgccgcgggc tggtcgcggc cgatttcgag atcatctcga cggggggcac
     5041 gctcaaggcg ctgaccgccg ccggcgtccc cgcgcgcaac gtttcggaat acaccggcgt
     5101 gccggagatc ctcggcggcc gcgtcaagac gcttcacccg atgatcttcg gcggcatcct
     5161 tgcccggcgc aacctcgccg acgacctctc cacgctcgcc gagcacggca tgcagacgat
     5221 cgacgtcgtc gccgtcaacc tctacccctt cgccgaaacc gtggccaacc ccagggccac
     5281 gcgcgaggag atcatcgagc agatcgacat cggcggcccg tcgctgatcc gcgccgccgc
     5341 caagaaccac ggcgatgtct acgtcgtcgt ccatccggag gattacgacc gcgtgctgga
     5401 cgccgtccgc tcgggccgtg aggccgacca catcgccctg cgccgccggc tggcggccaa
     5461 ggtcttcgag cacaccgcgc actacgaccg cctgatcagc gactacatgc gccaggcgtt
     5521 cgccgagccg ggcgacgacg cgcacgccgc cggccccgaa caagccccgg ccgcggccct
     5581 gcccgaccgg ctgacgctcg gctggccgaa acgccaggcg ctgcgctacg gcgagaatcc
     5641 gcatcaggcc gccgcgttct acgccgatcc cgactgcccg cccccctcga tggcgcactg
     5701 ccgccagctg cagggcaagg agctctccta caacaactac ctcgacggcg agacggcgct
     5761 cgaaatgatc cgcgaattcg aggaccccgc ctgcgtcatc ctgaagcacc tcaacccgtg
     5821 cggcgccgcc tggggcgaga actgcgtgga cgcctaccgc gacgcgctcg cctgcgatcc
     5881 cacgtcggcc ttcggcggca tcgtcggcgt caaccgcgag gtcgaccgcg cgctggccga
     5941 ggaactgggc cagatctttc tggaagtcat catcgccccg agcttttcgg ccgaggcgcg
     6001 cgaggtcctc gccgcgaaaa agaacctgcg cctgctcgcg accggcgagc tcaccccccg
     6061 cgcgacgcgg ctcgcctaca aatcggtttc cggcggcatg ctcgtgcagc agaccgacac
     6121 cggccggctg aacgacaagg aactgaaagt cgtgaccgac aagcagccga acgacgagga
     6181 ctggcgcgcc ctgcgtttcg cctggaaggt gtgcaagtgg gtcaagtcca acgccatcgt
     6241 tgtgaccgac gcccggcgca cgcgcggcat cggcgccggc cagatgagcc gcatcgacgc
     6301 caccaacttc tgcctccaga aagccggcgg caaggtcgag ggcggctacc tcgcgtcgga
     6361 cgccttcttt ccgttccgcg acgtcgtcga cctggcccac gcggccggca tcaaggcgat
     6421 catccagccg ggcggttcga tccgcgataa cgaaagcatc caggccgcca acgagcacgg
     6481 catcgcgatg gtcttcaccg gcatgcgcca cttccggcat tgaccccggg cccccacgat
     6541 ccctggctct ttttcctcat cgcggggctg tgcgtcgtgc tctccggcat cgccaagggc
     6601 gggttcggcg gcggcgtggg cgtcatcgcc acgcccctgc tctcgctggt cgtctcgccg
     6661 ctggtcgcgg cggggatcct gctgccgctg ctgtgcgcct gcgacctgtt cgccgtctgg
     6721 cactaccggc ggacgttcga ccgcgggaat ctcgtccagc ttgtccccgg cgggctggtc
     6781 gggatcctga tcggcgccgg cctgctgtgg catctgaaag gcgaaaccgc cgccgcggag
     6841 cgcgcgctgc gcatcctcat cggcgtgatc gccatcctgt tcgtcatctg gcaggccggc
     6901 cgcgcgtggt tcctgaagca tctgcccccc gcccggcccg ggacggtctt cggcgccgcg
     6961 atgggcgtca cggccggctt cacctcgacg ctggcccacg ccggcggccc gccggtcacg
     7021 atccacctgc tgccgcaacg cctcccgcgg caggtcttcg tcggcacgac cgtctggttc
     7081 ttcaccatcg ccaattacgt caaactggcg ccttacgccg cgctggggat gctcgatctg
     7141 gccaacctgc gcatcagcgc gatgctgctg ccgctggtgc cggtcggcgt ttacgccggc
     7201 atctggtgca accgccgcat caacgataaa tggttcaaca tcgtggttta cacgctgctg
     7261 ctgctggtcg gcattcagct cctcagcggc tacgacccgc tttccctcct cgcccgcata
     7321 acgtccctcc gataattcgt ccttcgtccc tcgtgatcgc ccgcgccgtc cggccacccg
     7381 ctgcgcgggt ttggcttgcc gggtcgcggt cacggggttt cggccgtcgg cctccacccc
     7441 gtgcttgatt cccatcgccc gcgatgcggg ctttttagag ggaaaaaccg gccacatcgc
     7501 gcgggtccgt tatgctctca ccgttccgcc gcgccgctga gctcctccgc gagcgagatc
     7561 acctgcggtt cggcccagta atccagctcc acgcgcgcca tcaaccgcac ggcccacccc
     7621 atgccgtaat tgatcgcgaa cggcacgatc gggttgagcg cccgctcctg cgccagccgc
     7681 gccgggtgca ggctgtttgc gatcagcgcc atcgcgcgca tcaggtggcc gcggtcctgc
     7741 ttgtgctgcg tcaacacgcg gtccagcagc ttgtcgtagc ccttgtccat cgcctgttgc
     7801 agcttgtcga tggcgctgcc ggccgccgcc gaggcatcga tctcgcgccg caacgcctcc
     7861 agctcttcct tctgccgcgc gcgcagcgat tcgattcgcc cgatctgtcc ctggcccaga
     7921 tcgcgctgca ggatcatctc aaccagctca tccgcgtcgc gcgtgagggc ctgctccagc
     7981 gccaacgcgt gcttttcgag ccgccgctcc acgcgcggct ccagcagcgt cacgctgggc
     8041 cgcggccagc gcaccggcgt aaacaccccg aacgactcgt agaccgcctc cacctgcgcc
     8101 agataggccg cctcgcccgg accgaccacc tgcgccacgg tcggcagcgc cgaatcctgc
     8161 accagcggcc gcgtcacgac gttcgtgctg aagcgctccg gcgcgcgctc cacctgccgc
     8221 cgcagctcat ccagcgccac gtcctccggc gcctcgtctc cgccgccggg cgctacgggg
     8281 cggacccgct ccgcgccatc gagccgcaac gccaggcggc gccgcgcgcc gtcgatccag
     8341 aacacgttca ggctgtcggg cgcgcgatgc agttgcgcct cgaaccgcgc cgcctccagt
     8401 gcccgcgtcc gctccagaat ccgcgccgtg gactcgcccg gccgcgccag ttcggtctgc
     8461 acgatcggcg ccgcgcgccg gcgcacccac tccatcagcg gcgagacgat caccagcccc
     8521 gtcccgcgga acagctccag catcaaccgg cagaagtcat cctcgagcgt gacctccgcc
     8581 ccatcccccc cgcgcagccg cgccagcacg ccgtcgcgga attccgtcgt cagcgtgctc
     8641 atctcgatct ggtgcgccag gcgcgccagt tgctcgccga tcctgagccc gccgaccatc
     8701 cggcccggcg tcgtctcggc cgccagcagg aactcctcca gtcccccctc gtgcccgggc
     8761 cagtacgccc gccggatttc atcgaagtca tgatcctccg acgcgaccca gaagacggga
     8821 acgaacgtca gatccggatg ccgctcccgc agccggccgg cgagccggat cgccgccagc
     8881 gtcttgtaga ggaccagcag cggaccggcc agcagcgccg gctgctggcc cgtgacgacg
     8941 acgcgcgcgc gcggcgtccc cagttcgcgg atcgccgcca gcgtcgcctc atccgccccc
     9001 cagcgccggt gcgccgtttc cagctccgca agctcatgct ccgcgagcgg cgagggcgaa
     9061 tgccccgccc gctgcgcaag gcagtcctcc agctcgctcg gatgccagcg cagccatcgc
     9121 tgtcccggcg ccggctcgcg cgccttcaac tggtcccaca cggattgctg aattctgacg
     9181 gccatctcca tccccggcgc cggctggatt gcggacccct gattgtagga agccgcgacg
     9241 cgaactacaa ccacgccgcg cccggccggt ttttcttcga cccgcccgac gccgtgtgct
     9301 atcacgtcgt gtgccttgcc cgagcgcgcc ctgccgcgac ccgcccattc cgccggaatc
     9361 gacgcgcgat gcccccttcc gccaaacgcc gccccgtcgc cgccgccgcg cccaaggcca
     9421 ccgtcttcaa cacctcccag ctcagccggt tcgaccgcga acgccggcgc cggctcggtg
     9481 tcgagttgct ggggggcgtc gacgaggccg gccgcggcgc cctggccggc ccggtcgtct
     9541 cggcctgcgt cgtcctcgag ccgggcaccc gcctgggcgg cgtcaacgac tccaaggtgc
     9601 tgacgccgga ggatcgcgag cgcctcgtgc cgcagatcat ggaacgcgcc gcggcctgga
     9661 gcgtcggctg ggcctctgcc gccgaaatcg accggatcaa catcctggag gcgacgctgc
     9721 tcgccgccga ccgcgccctg cgcgccctgg gggaccgcac cccccagcac atgatcaccg
     9781 acttcctcaa gctgcgctgg gccccgtgcc ccgtcgagcc gatcgtcgac ggcgatgccc
     9841 gcagtctggc cgtcgccgcc gcctcgatcc tggccaaggt cgcccgcgac cggatcatga
     9901 ccgccctctg cgcggaatac ccctgctacg gtttcagccg ccataagggt tacggcacgc
     9961 gggcgcactg gcgggcgctc gacagccacg gcccctccac gctgcaccgg ctgacctaca
    10021 atggcgtatg cttcttcaat gcctcgcccg atgtccgctc gcgccgtcgg cgctgtctgg
    10081 aatccatccc gagcgaaccg cagccgcccg actggctcga ggtgctgacc agcccccccg
    10141 accaactcga cccgctcccg ttccttcccg aggcggaatg ggagctatcc gtgtcttacg
    10201 cttcagaaac gtccctcgtc ccacggatga aaatccgtgt ttcagaaggg acataaggga
    10261 cataagagac aaaagggacg ctggcagacc attttcatcc tttcagggtg acgcgaaggc
    10321 gtcgtgaaca gctcacgcta aaatcctcac ggctctcttg agcgcccgcg ccaacaggct
    10381 tttcacgcat gtcggccccc tcccgtcggc atcaggctgt taatccccgc acgccttcca
    10441 gaaacttgct ttcgagtcct ttcgtgcttt tcgtgagcca aattcgcgca aatttnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaac ttgctttcga gtcctttcgt
    10621 gcttttcgtg agccaaattc gcgcaaattt cagccgcccc cgcccgcatt gccgatcccc
    10681 ccgccatctg atagaatccg gcgggatgtg tggaagcgcc cgggcgaggg gttgacacag
    10741 tgatcgaata cctgcgcggg atcctgctcg aacgccaggc cgaccatgta gtcgtcgacg
    10801 tcggcggcgt cggctacggc gtggcggtgc cggcctcgac ggcgcagacg ctcggcgacg
    10861 ccggctcgca ggtcgccctc tgggtccgga cctacgtgcg cgaggacatc ctgcggctct
    10921 atggtttcaa aacccgccac gagcgcgacg tcttcgacat cttcctcggt ctgagcggcg
    10981 tcggcccggg caccgggctg gcgctgctct cctccctgac ggtggcgcag attattcagg
    11041 ccgccgcggc gggcgatgcc gcgcgattca agaaggtcaa gggcatcggc cagaaaatgg
    11101 ccgaaaagct ggtgctcgaa ctcaaggggc gggtcgaccg cctcgtcgcc gggctccccg
    11161 aggaactggc gcgcgaggcg accgccgagg cggcgccgct gagcgacaac gcccgcgacg
    11221 ccgtggcggc gctagaagcg ctcgacgtcc gcccccagca ggcgcggcgc gccgtcgttc
    11281 tcgccctcga agcgctcggc gaagacgcca gcgtccaaca actcgtcagg gaggggctca
    11341 agcaccgccg cgcgacggcg ccttaagccg gtatcatggc cgattccgat agttccgtgg
    11401 cgaatcaccc atcgatcctg cgggccatgc cctcgctgcc ggcgatgttg ggcttcgcga
    11461 tgcccaacgc gatcagcttc cagatcgcgc tggggccccc gctcgtcctg atggcccgcc
    11521 actggggcgc ttcctcgttc tacatcggcg ccatcgccgc cctcgtgccg ctgctcacga
    11581 tgacgcagct ctacatggca ccccgcgtcg aatacctcgg cttcaaacgc gtgatgctgg
    11641 cgggctggac cggccgcatc gtcaccctga tcctcaccgc cgccctcccc ttctgctccg
    11701 gcttcttgag ccgccgcttt caactggagg ctctcttcgc cttcatgttc ctgtttaacc
    11761 tgctgcgcgg gctggccacg gccagctgga tgccgtgggt caaggaactg gtgcccgagc
    11821 gctggcgcgg ccgctacctc tccgccgaac aggtcgtagt caacatcagt tcggcgctga
    11881 ccctgttcgg ctgcggctgg gtcctgggcg acgaccccgg cgatttccag ttcggcttcc
    11941 tgttcatcgt ctcgttcgtg gccggcatga ccagcctgta cttcctcaag cggatcgact
    12001 gcccgccgcc gctcgagggc gcccccacgc tcgaaccggt ccgccgctgg gtcaaccgcg
    12061 tctggcgcgt caagccgttc cgccggctca tccgcctcaa catgctgtgg accttcgccc
    12121 tgggggcgtg gggcacgttc accgtcgtct atctgcgcga cggcctgcat ttccccgagc
    12181 ggatcattct ctacatgagc gcttccagca cgctgggggc gctcctcagc gcctgggtgt
    12241 ggggcctgat ggccgaccgg ttcggctcgc gaccggtgat ggccctgacg tcgcggctga
    12301 tcctgctgac cgccctctgc tggctgctgc tgacgcttgg cctgatcccc ccggccgtcg
    12361 gcaccgtcct gattctttac atcctgctcg gaatgggcac ctcgggcttc ggcgtcgcga
    12421 tcaaccagta ttcactgaac aacgcgccgg ccggcttccc ggtcctcgcc ctctcgctct
    12481 tcgccgccac ctcgtcgttc agcagcgcgc tctccccctt cctgtggggg gcgctgctcg
    12541 acctgctggc gcccgtccgg cctcgcgtcg gcccgctcct gcttcaccag tatgtgtggt
    12601 tctacgccct gatcgcgctg atgatcgtgg ccagcaagca tctgatccgc caactgcccg
    12661 atcacaaggc agccgccacg cacatcgtcc tctatcacat gatcacggat tacccgctgc
    12721 ggacgctcta tgccgtgcag gggatgctga ccggccgcac acgccgcgaa ggtcccccca
    12781 cccgcgggta agtgtggctg gtgcatcgag gaatttaatg atcgaaccga tagatcaaag
    12841 tttcgtcatc acggaccgac agggacgccg gtcccaggcc gcggttcttg attggtcttc
    12901 acgagagacg aggacgaggg acgatgcttg agtgaggggc gggcgtgtgg gggcactcct
    12961 cccaatcggc cagcagcgag cgccggatcg acggtgccgc gtcgtgattc actccgggtt
    13021 ctcctggctc gtgctccatc ccagcgttcg cttcaacgca cgccgccagc cggcgtagcc
    13081 ggcctcgcgc tgatcggccg accagcgcgg catccactcg gcatcggcct gccagtggcg
    13141 gcgcagatcg gccagaccgc gccatgcgcc ggcggccagg cccgcggcgt aggccgcgcc
    13201 aagcgccgtc gtttcagtta ccttcgggcg gaccacgggc gtcccgagga tgtcggcctg
    13261 catctgcatc aacaggttgt tgaccgacgc gccgccatcg acgcgcaaca cctccagcgg
    13321 ccggcccgag tcgcgcacca tcgcctcgac gatatcgcgc gtctggtaac agatcgcttc
    13381 gagcgtggcg cgcgccaggt gcgcccgcgt gtggaagcgc gacaggccga cgatcgcgcc
    13441 ccgggcgtcg ggccgccagt gcggcgcgaa cagaccggaa aaggccggca cgaaataaat
    13501 cccgccggca tcggggaccg acgcggcgag cgcctcgatt tctcccgcgt cgcggatcac
    13561 gccgagctga tcgcgcagcc actgcaccgc cgagcccgtc accgcgatcg ggccttccag
    13621 cgcgtaggtc aggccgcccg cccccagccg gtaaagcgcc gtcgtcagca gccccgccgt
    13681 cgaccggatt cgttcggttc ccgtattgac cagcagaaaa ttccccgtcc cgtaagtgtt
    13741 cttcccctcg ccggggtcga agcagacctg gcccatcgtc gccgcctgct gatcgcccag
    13801 cgcgccgcag accggcgctt cgccgcccag cggcccgccg gcgcgcgtca ggccgtaggc
    13861 ttgcggatcg gacgacggcc ggatcgccgg cagcatctcc atcggcacgc cgaacaggtc
    13921 ggcaagctcc gggtcccacg cgcccgcgcg caggtccatc agcagcgtgc ggctcgcgtt
    13981 ggacgggtcg gtcacgtggg cgcccccgtc ggcgccgccc gtcaggttcc agatcaccca
    14041 ggagtcgatc gtgccgaaga ctgccgcgcc gcgcgccgcc gcacgccgca gctcggggtg
    14101 ctcggtcagc atccattcca tcttccccgc cgcgaagtag gtcgagagcg gcagcccggt
    14161 ccgttcgcgc acccgctcct cctccccgcg ctcgcgatgc gcgcgcatca gttcctcggt
    14221 ccgcgtgtcc tgccagacga tcgcgttgcc gcagggctcg cccgtgcgcg gataccacgc
    14281 caccgtcgtc tcgcgctggt tggtgacgcc gatcgccgcc agatccgacg cgcgcaggcc
    14341 cgcccgcgcc atcgccccgg caatcgccgc ctcggtattg cgcaggatct cgcgcgcgtc
    14401 gtgctccacc cagcccggct gcggcatgat ctgtgcgtgc tcgagttgat ggcgcgcaac
    14461 ctcccgcccc tcctcgtcga aaatcatgaa ccgcgtgctg gtcgttccct gatcgatcgc
    14521 gccgatgtag ctgcccatcg ggggttctcc tttcatgact gaacggtgcc ggcgattatc
    14581 gtcgcacgct ccgtttgcgc cgtcatcccc cgaccgtttg ggatcgcgcc gggcccgacg
    14641 gtgttggctt tcgacggtcc gttcgcctac tatggggccg gtgcccgcgc cgatcgattc
    14701 cagcccgtcg catgaggtag acccggtgat gtcgccgctc gcttccgccc tcccgcctga
    14761 tcgccggccg cgccgccggc gcgcaagatc cccgcgggca cggcggctgc tggcgctctt
    14821 cctgctggcg ctggccggcc gcgcgccggc gcaggtcgag attgacggcg acttttccga
    14881 ctggacctcg atcccggcga tgacctgcgc cgactcctca gcacacgatg gcgagcttgg
    14941 ccgggtcgcg cagtggaaag tcgccgccga cccgcagttt cttttcgcgc tgatccaatt
    15001 cgaggcgccc cgcccgctgg tcaaccccgc cggctccacc gagctccagc ccggccgctg
    15061 ggacgacttc ggctacctcg acctcgacat caaccgcgac ggccagtggg actaccgcgc
    15121 ccagttgagc gaggggaaac gccccggcct gaacaacctc gtcgtgatga acctcgatgc
    15181 cgacggcaac ccgcgaggcc gattgctgta tccggaaggt cacaagaatt atttcccgct
    15241 cggtccgcgc gcgcggctga gcgccgatgg gcgcacgatc gagctgcgca tcccgcgccg
    15301 gccgctgcgg ctggatgtcc ccgcgacgat cgatctgcgc gtccgcacgc gctaccgcga
    15361 ctcggtgcgc ggcgggggcg gctgggtcac cgaattcacc cccggcgccg cgggttgcgc
    15421 gtttcaactg ggcggccgcc tgcggccgac cgacgatccc gtcctgacgc ccggcgcggt
    15481 caatctgccg cccgcccggc gcgccgaggg agcggaggaa ctcgagatcg ccggctcgca
    15541 tgaacgcctg gcgccttcgg cccgcccctt cctgatcccc aacctgccgc aggaatccgt
    15601 ccgaaccgct ccgccgatcg atccgacgcc gctgcccgaa gcggcgccgc tgagcgacga
    15661 ggccgcggcg gcggcgcgct cgatgagcga agcgatgacc gcagtggaac aggcgcgcca
    15721 ggccgggatc gaggaggcgc tggccgttgc cgtgacgccg acgccggtcc cgaccgcgac
    15781 gcccaccccc acgcccgatc ccgtcgccgt ccaagcggaa gcgttcgatc aggaagttga
    15841 ctcactgatc agctccattg gaacagaacc cgggcagccg gccgccgcgc cggcggccag
    15901 gccggaggtt ccggcgccgg gaatggagct tgtgccgccc atcgtcgcgc cgggagcgga
    15961 ggtcgccccg ccggcgcccg aagcggaact ggaggcgcct tcccccgagg aattgcagag
    16021 aacgttcgat cagaatatcg aaagcctgat cgatgcgatc cagagcgaag ggggcgcggc
    16081 gacgggggct gtccgctcgc cggttgccgt cggcccgacg ccggcgcctg aaccctcccc
    16141 gacgccgacc cccgaaccga cgtcgacgcc gacacctgaa cccaccttga cgccgacccc
    16201 ggcgcccacc gtttcaccga cgccgatccc cacacccgac ttcacgccgc ggcccacgcc
    16261 cggtccgcgg cccacggcca cgccgctccc caccgccacg ccgccgccgg agtcaacgcc
    16321 aacgcccgag ccgacgccgg cgcccgcgcc ggaaccaacc gccgaaccga cgcccacgcc
    16381 gacgccgagc ccggtgctca ccccgacgcc cacgcccacc ccggagccgc gcggcctcaa
    16441 catcgccgac gttccctttg cccagcccgg cgacgaaccg atcacgctcg acgccgatcc
    16501 gtcggactgg gagcatgccg actacgtcca tgtcccctcg gccgatgcgc gttccaccgc
    16561 cgcgcatccc attgcgctgg tcgaccgcat cctggttgcc tcatccagcg gcttcctcta
    16621 tacgcggatt gattttgcgg agtcgcgctt cgcgctgctg ccccccgccg acggctggcc
    16681 gatcgatgcc ggggcctacc tggcgatgga tgtcgatctg gacggcgact gggatttcga
    16741 ggtgcaggcg ctgcgcggcg cggcggggtt cgtcccggcc caggtggtca acgtcgacgg
    16801 tgccgtggtc ctcgccgcgc aggaagacga aggcgcgcgt cccgatgggc cgatcgcgcg
    16861 cttcgacgag gaaggccgtt cgctcgagtt gcggctcccg cgcgcgccgc tctggctcga
    16921 tggcgacgtt cgcctgcgca tgcgggttct cgtgcgctac cagcttgaac cggaggccgc
    16981 ctggcggcag atgcaatacc ccgacgtcaa ggacggctgg ttcgccttcc cgttgcggaa
    17041 ggagtagcgg gtcattcgcc gccgagccat cggcgccgct ccgggggcgt gagccgggcg
    17101 agggcctcgc gcgccgcgag ccgctcggga gggacgaaga cgcgcgtagc ccacaggttg
    17161 cggtgcgagt cgaggattgc ctgcagcttg atgccggtca gcgcgtcgtc gcggcagtcg
    17221 cgcagcggac ggatcgcgcc ccgccacagc accatcgtgt cggccagctt catcgccatc
    17281 ttcagcggcg ggcaatggat cagcagttcg ccggggtcca ggcccagttc cgccgcgcgc
    17341 cgttcctcca ggcggcgccg ccgggccgcg tcggcgtgca ggcgcgcgag caggtgctcg
    17401 accgcgggcg cccgatcctc cccatccgcc gcgtggggcg gacccgcgac ggtgccggcc
    17461 actccctcag cgcccagcgc ctcgcgatcc cagcgtgccg ccagcttcca cagccgccgc
    17521 tccaggacgg cgcgcgccag tccggccgcc tgcggcggct ccccccgcgc cagccgccac
    17581 agcagcgtgt cgtcgcccag atcgaagagc tccgcggggt cgagcgtccc cgccgcgagg
    17641 gcccgcgcga cggccgcccc caccatcgtc cccgccacca gcttggcgtg gtggaagtag
    17701 acccgctcgc tcaggacgta gcggttgtcg agcaggcgga tcagttcgcc gaggacgtcg
    17761 cgccgcggcg cgtccttatt ctccttccag agctggacca cgagccgccg ccgcccgccg
    17821 tgacgccgca gcgccagata gcgatagaac cggtcgcccg cctcgacgct caggttgcag
    17881 aagaacgcgt cgcgcgccag gtagtccagc aggtcggcgc agatcgtatt cgtcaccagg
    17941 tcgaggatgt aaacgtcgtc ggccgccggc tcgcactggc ccgccgcggc ctcgaacagc
    18001 cgcagcaggc gctggtagag ctcgccgccc agggccggcc gcaggagccg gcccgccgcg
    18061 ctctccgggc cgacgaatcg ctcgatccgc tcgcggctgg catcgtggcg cgcgaagagc
    18121 cccagttcgt cctccagcgt gtggccgaac gggatgtggc cgatgtcatg cagcagcgcg
    18181 aagagccgcg ccagcagctc ttcctcgggc gggatgtcgc gctcctcgct gtgcgcgcgg
    18241 gcattcccgc ggatcgcctc gatcatccgc cgcgccgcgc ccagcgtccc cagcgagtga
    18301 tcgaaccgcg tgtgcagcgc cgtcgggtag accagatacg ccgcgccgag ctggcggatg
    18361 aagcgcagcc gctggaacgc gggcgtgtcg atcagccgcg tttcgagccg cgtcagttcg
    18421 acgtcgccgc tgaccgccag ccggatcgtc ttgtaggtgg tcaggtccag ccccgtgttc
    18481 aacccgggca gcgagggctg cgaatgcgcg gcgagcgtgc gacgcggttt cggcatgagg
    18541 cccatgcttg accagccggg ggcggattgt aaaggcccag ccagccgtgg cgctttgccg
    18601 cccgtgaaac gccgcttttt ccgtggtttt ccttgcgggc ggtttgttat cttcaaaaca
    18661 tgaacgaggt gtttacaccg atcccccagg ctatcgaagc gatccgcaag ggcgagatca
    18721 tcatcctgat cgacgatgag gatcgcgaga acgaggggga tttcgtcttc gcggcggaac
    18781 acgccacgcc ggaaaagatc aacctgctgg ccacgcatgg ccgcggcctg atctgcgtcc
    18841 ccgccgcgcg cgagcgcctg gcgcatttcc tcctggagcc ctaccccgaa aaccagaaca
    18901 ccgcgctgat ggggacgaat ttcaccgtct cggtcgatgc gcgccacggc gtcaccacgg
    18961 ggatctcggc gccggaccgc tgccggaccg tgcgcgtgct ggccgatccc cggagccgcc
    19021 cctccgacct ggtgcgtccc ggccacatcc atccgatcgc ggcgaaggat ggcggcgtgc
    19081 tgacccgcgc cggccatacc gaggcgacgg tggatctgtg ccggctggcc ggactggagc
    19141 cggtcggcgt cctgtgcgag atcctgaagg acgacggcga aatggcgcgc ctgcccgatc
    19201 tgatccagat cgcccggcgg ttcagcatgc cgatcgtcac gatccgcgac ctgatcggct
    19261 atcgccagcg gaccgagaaa ctggtcaagc gcgccgttac gacgcgcatc cccaacgcct
    19321 acggcgaatg ggaactgacg ctgtacgaag acctcgtcaa cgacgaactg cacctggcga
    19381 tgacgatggg cgagatcaac gacgagccgc tgctcgtccg gatgcacagc cagtgcttca
    19441 cgggcgacac gctcgggtcg ctgcgctgcg agtgcgggcc gcagctcttc accgcgatgg
    19501 acgcgatcag ccgcttcggc cgcggcgtca tcgtctatct gcaccaggag gggcgcggca
    19561 tcggcctgaa gaacaagctg ctggcctatg cgttgcagga tcaggggctc gatacgatcg
    19621 aagccaatga aaaactgggc ttcaaggccg acctgcgcga atacggcatc ggtgcgcaga
    19681 tcctggtcga cctgggcgtg cggcggatcc gactgatgac gaacaatccg cgcaagatcg
    19741 tcggcctgga ggcgttcaat ctggaaatcg tcgaacgcgt gccgctcgag gtcggccact
    19801 gcgagttcaa ccagcactat ctggagacca aacgccggcg gctggggcac atgctgagcg
    19861 gcgccgccgt gctcggcgcc gcgccggccg atgcgaccga cgatggcgat tcggccacga
    19921 agcaagtatc ctgactcagc ttgaccgaac ggatcggggg gacggcagat ggcaagggaa
    19981 cgcgcaatgc aggtgctgga gggggactac gacgccaggg gattgcggct ggcgctggtg
    20041 gcgagccgct tcaatgaatt catcgtcggc aaactggtgg agggggcgct cgacgcgttc
    20101 gtccgccacg gcggcgatcc cggcgcggtg accctcgcct gggtccccgg ctcgctcgaa
    20161 ctgccgccgg tgctggcccg gctggcgcag agcggccggt tcgacgccgt catcgcgctg
    20221 ggctgcgtca tccgcggcgg caccgaccac tacacctacg tggccggcga agccgccaag
    20281 ggggtcgcgc aggttgccct gaacagtgcg gttcccgtaa ttttcggcgt cctgacggtt
    20341 gattcgatcg agcaggcgat cgagcgcgcc gggaccaaga tgggcaacaa gggggccgat
    20401 gcagcggtcg ccgccatcga aatggcgcgg ctctacgccc gcttgcccgg cgcaaaaaca
    20461 ggcaaggcgt gatgcccatt cacggcggcg ccttccctcc gccgccggaa gcggtgcggg
    20521 ccgcgcggcg aacgctgcgg cgccaggctc cgccgctgat tgcaagattc ctgaatcatc
    20581 tcacggcctg cccggcgatc gccccggatc gggtcgacac ggcgtgggcg ctggcggcgc
    20641 tcgcgcggga gatggccggc gcgctggccg ccgcgcagca gacgggcgga cggcccgtcg
    20701 tggagctcgt cttcgccctg caccggcgcc tgggccacgg cgtcgcgacg cccgctccgg
    20761 cgccgaccat cgatctggcc cccgtgctgc gcgggctggc gccgtatgag gcctggctcg
    20821 cgcggctgac ggcggactcg cccccgtggc gcgcggcgct gatgcgctgg atcgacgtgc
    20881 tgagcgagcg cctcacgatg caccgctacc ctgaccccta ccgccgccgt cagcacgccc
    20941 acgcgctgct gggccgcccg ctggaactgc tcgccgcgcc cgcggtcggc gcggccgaga
    21001 ttgatccggt cgcccttgcc ctgctgcggt tctgggatct ggcgcgcgcg cgcgcgtacg
    21061 ccggccgcgg ctggaaccgc ctcgactccc ccgccgaccg cctgggggga ttctacggcc
    21121 tcgccgacga ggcccgcccg ccgacgcccg aggagatccg gtccgccgcg gcggcgctgc
    21181 atgctgagtt gaatggggag tggcctgcct gggccgaacc gctccgcgcg gacctgcaag
    21241 cggccaccgc gagctatctg gaatccaatc gatggccggc ctggaccacc gccgaccgcc
    21301 ccgccggcct catccaacgc atgtttggcc gtgcacaggg atagcgcacc cgctcatcgc
    21361 aaacccaagc cgcggagcgg gtgaccggaa caaagcgaac gctctgtaag ctgagtgtta
    21421 gatcactgaa ggaacgcttc cctgaattcg acccgatgcg ctttgagcaa ggcgatcagc
    21481 tcgtcgcgaa aactccgggc gtgatgatga atctcctgcc gctgaatgta gcacgcaagc
    21541 ttggcatctc cggattggct gaccgtgaaa caggcgtagc cattctgcca ttgaaactcc
    21601 ccgagttcag ggatcgtgcg atgcacccag cctgacgatt ttgatttgat cgtgcgaagc
    21661 atcgtggaga cagaaaagtc ctgatgcatc ttggcataga tgtgcacgtg gtcctcggtt
    21721 ccaccgatac acagcgcaat accgttcatc tcgcggacta tgccgccaag atatgcaatg
    21781 aagtcgacgc cggatgtcag ggtctgtcag aaatggcctg atgtgcttcg tgccgaacac
    21841 catgtgatag aggagattgc agtaggattg ccccatggaa gatcgcctcc ctgggaagga
    21901 tttccggcca cccgctcctc gggttcggct tctcaccgga cggacacagg gtttggcctg
    21961 cggcctgcac ccggtgcttt actccaagtg cccgctgcgc gggctctcac gctgtgcgcg
    22021 accgccaagt cagtcgtcgt ctcgacccgg ttggtcatca tagaacgttc gcagcgcatc
    22081 gagcagttcg ctctggcgcg cgaaatggat ccgctgcgtc ggcaggctgc gcaggaagat
    22141 ctggccgtag cgccgcgtcg cgacgcgggg atcgcagatc aggacggcgc cgcggtcggt
    22201 cttcgtccgg atcagccggc caaaaccctg gcggaacttg atgaccgcct gcgggatcac
    22261 cagatgataa aacggatcca gccgccgccg gcggagatcc tcaatgcgcg cctcggtgag
    22321 cggatcgccc ggcaccttga acggcaatcg cgtcagcacc aggcaactga gcgcctcgcc
    22381 cgccacatcg accccctccc agaagctgga ggtggcgaac agaatcgagc cgatttcgtt
    22441 gcggaaggct tcggtcagca tcgagcgccc gatcgtcccc tgccgcaggc agggatagcc
    22501 gcgcgcctcg agccccggcg ccagccggtc aaacacccgc ccgagcaagg cataggaggt
    22561 aaagaggacg aaggcgcggc cgcgcgagat ctcgagcgtc gggccgagga agccggtcaa
    22621 ggcctcctcg aagcgagggt cggtcggctc cgccatgtcc attggcaccc ccacgtagac
    22681 ctgccgttca taatcgaacg gcgtcgccag gatgagggcc cggaacgggc gggcgcgcgg
    22741 cggcggcaac tcatcgggtt cagcggaggc atccgagggg agtgggggga tcagctcaat
    22801 gatctcggcc gcggagggcg cgttttcggc gaacggttcg gccggcggcg gctccagctc
    22861 ttcgccgccc ggcgcggcgg acgcgctcgc ctccgccgcc gtcggcccga tcagccccag
    22921 ttgcagcggg tcctcggcgc cgatctggcg cagcagataa tcgaaacgct gatcgaccgt
    22981 cagcgtggcc gaggtcatga cgaccgtccg gcatcgacgc agcagattct cccgcagttc
    23041 ggcggccatg ttgagcggag cgagacaata ggtcacgtcg gggagccggc cgcccggccg
    23101 acggcgatac tcgatccagc ggcactgtcc ctcggcgctg cccaggaact tgatcagccg
    23161 cccaatcgcc gcctcgactt tgttcaggct ggactgcagc tcaagcaacg gcgtggccag
    23221 ttccggcgac gcctcctcca ggaagggcag gagcgcgcgg ccgacggcgc gcaggttctc
    23281 cagatacggg cgcgcggcgg tgacgacggc gcgcagcggc gcatcgattt cctcgcgcca
    23341 gaacggcccc gccagatcct cggttttgat ccggcgcttg atctcgagcg gctggtggag
    23401 cggcgtccgc tccgtgcgct cgagcgcttc ggccaaccgg ccggtcgcct cctccatcgc
    23461 cgtcagcagc tcgtggtgct ggagcggcag atcgcgcccg agcttgagca gcagttcatc
    23521 gtgctgctgg ggcgccacgg ggtattcgcc gttgtggatc cggccggcga gccagatcag
    23581 caacccatcg ttgcgccgcg tgttgaacag acgccgcatg cagtaggcca ggctgccgcg
    23641 actgacgcgc acgccgaagt agctggtggc gacctcttcc agattgtgcg cctcgtccac
    23701 gatcagccgc tggtacggcg gcaggacggc cgtcgccgag tagttctccg tttcggcccg
    23761 gatcgccatg tcggccatca gcaggtggtg gttgacgacg agcacctggg cccgggcggc
    23821 gcggcggcgg gcggtgtaga aaaagcaggt atcgtaaaac gggcagcgcg tccgcaggca
    23881 gttgtcggcc tccgacatca cgctctccca cacttcgggg tccggggtga agggcaggtc
    23941 ctccaggctg ccggtgcggg tggtgcggat ccaggcctgg atctcggcca gttgggcggc
    24001 cttctcgccg atttgcagaa aatcgggatg ctcgcgcaga tactgtgcct tgcgccgaca
    24061 gaggtagttg ttccggcctt tcatcagcgt cgcctcgaac tgcaggccgg aattctgcag
    24121 cagcagcggg atgtccttct cgatcaactg ctcctgcaga ttgatcgtgc cggtggcgat
    24181 gacgaccttc tcattgttgc gaatcgccca ggcgatcgcc ggcagcaggt acgccatcga
    24241 cttgccggtg ccggtgcccg cttcgatcag ggcgatgccg tcggaattga acgcctcgcc
    24301 gaccgcctgc agcatctcga tctgttgagg ccggtattca aaaccggcca ggcgctcggc
    24361 cagtcggccg cccggcttga gccagttggc cagcttgccc acgctgatcg gctgaagggt
    24421 gggcggctcc agcgcgcgca cgatgatgcg cagcgcagtg cagtcgttgt tgatgatgta
    24481 ggaaccgacg ccgcggccgc ccagttcgct ggcgacggcg atgtccgcct cggagggctc
    24541 gagccgaccg gaagggtggt tgtggatgca gacgtcgccg gggcgggcgt agggcagcag
    24601 ggccgggacg gcgccgcgat tgccgaaggc gagcggctca accgcttcga cgacgcgggc
    24661 ggggttgagc cggccgatga agaacacttc gccgccgccg ttgccggcga tgacggcggc
    24721 catctgctcg cggcaggccg cggtgatcag gcggtcgagc tcgccgggcg tggtcgcgat
    24781 cgcgcggaag ggttccttgg gagtggccag cgccatggca tcgcggtcgc ggcgggttgg
    24841 gtggcgggtc cggcgctgcg cggcggcagt ccggcgaact gtcaatttat cacgcgacgg
    24901 ggtcagcgcc aagcgccaaa aggcgaataa aaggcgaaca tggctcccgt ccgacgaggc
    24961 ggggaggccg gtgaccgacc gcattaccga taccgatagc gataccaata gtgaggggcg
    25021 agctgctggc ggatgaagtt ggcccccgga atgggcggtt ccgggggccg tgggggatga
    25081 ggtgatgtgt gggagagaaa ctttagcggc cgcgggttcg gcgacggggc ggaagggtcg
    25141 agccaaccct gaacaggaaa acctggccgt gatatccatc tgatcggcgc gcctggatga
    25201 attcatgagt aattttttcc gattattcag ttaagacgat tccgttgtcc ttattcggcg
    25261 cttttcttct ttggagggct gccggtcgaa tggctcagga accggcggtt catggcgggg
    25321 aatcgccggt tgacgccgtg cgacgggatt gattaaatga tcaagcctat cgagactgcc
    25381 gaccttcgac gcggagaggt ggccgagcgg ctgaaggcag cagattgcta atctgtcgtg
    25441 gggggttacc tccaccgcgg gttcgaatcc cgccctctcc gccatttctc gcctcccttg
    25501 attcagattc gccccttttg cgggcccccc ggattggggc atggctcggt ttgtcccccg
    25561 ggagcgcccg ctgcggcccg tagttcgcgc cgtccaggcc gagaagaaga tttcgcggcg
    25621 cgcgggcgtg tgcgtagaat ggggcgtcgt gcgcacgaaa ccatcccgcc gcgcccgccg
    25681 gtcgcgcgag cgcgagcccg ccgaaaggtt tcacccatgc ccgtcgccat ggttctcctc
    25741 tgcctgatcg cgctgattct gccgacgccg tccgcctggg cgcagccggc cgcgccggaa
    25801 gcgccgccgg acgaaccgga gtggacgctg ctggcgccgt tgcgggtggt gcggctcgac
    25861 aacggcatga cgttcctgct ctatcccaac gaccgggcgc cgatcttctc cggcgtgatc
    25921 cgctacgacg tgggggggaa agacgagatc ccgacgcaga ccggcatttc gcacatgttt
    25981 gaacacatgg ccttcaaggg gacgtcgcgg ctgggcacga ccgattacgc ggcggagcag
    26041 gaagcactgg cggaagtcga gcggctggcc tacgcgtacg accggctgcg caatcagctc
    26101 gcgcgcgacg attcgccgac gacggcgcgg cttgacgcgc tgcgcgagct tgcgatgcag
    26161 tttcaggccg cgcaggccgc cgccgggcag tacgtcgtca aggatgagtt cgacgagatc
    26221 tacagccgcg aggggggcag ccagatgaac gccaccacct cgcccgatgc gacaacgtat
    26281 ttcatcagcc tgccggccaa ccggctgccg ctatgggcgc ggatggaaag cgagcggctg
    26341 gccgaccccg tcccgcgcga gttctaccgc gaacgcgagg tgatcctgga ggagcggcgg
    26401 atgcggctgg acaacagccc gctgggccga ctgtgggaga tcacgatggc cacggcctac
    26461 gtcgcgcatc cgtatggtta cccgacgatc ggctgggaga gcgacatcgc gaacctgaaa
    26521 gcgaccgagg cgatggcgtt ctaccggcgg cactatacgc cggaccgggc cgtcggcgtg
    26581 ctggtcggcc agttcaatcc ggaggaagcc gaggcgctgc tgcgcgagac gttcggcaag
    26641 ctgcccccgg ccccggagga tcttgccgaa caccggatcg tgcccgagcc ggagcagatg
    26701 ggcgaacggc gcgcgacgct ggaatttccg gcgacgccgg cgttgctgct ggcgtggcac
    26761 aagccgccag cgcccggcaa ggcggatgtc gcggccgaac tgctggggca gatcgtggcg
    26821 gggggacgca gcgcgcggtg gtttgagcgg ctggtcaagg aggaacggct ggccgccgag
    26881 gtccacacct tcaccgggcc gggcgatcgc gagcccaatc tgttcatgat ctatgcgacg
    26941 ccgcagccgg gccatacgct cgacgagctg gaggcggcgc tgcgcgagga ggtccggcgc
    27001 gtccgcaccg agccggtcag cgcggaggaa ctggagcggg cgcggaagaa cctgcgcgcc
    27061 gacacgatcc gcgcgctgca gaccaacctg ggcctggccc ggacgctggc cgagtatgcg
    27121 cagatcggcg cccccgcgcc gggcgagatc gatccgtggt atctggagcg ccggctccat
    27181 cagcttgaag ggttgacggc cggggatctg ctcgccttcg ccgccgagta tctgaccgat
    27241 gccaatctga cggtcggcag cctgacgccg cccccctcgg cggaggaaac gccgccggga
    27301 gccggcgcga gggggggcac agattcggga gcatcagaag agacacaagg gacataaagg
    27361 aatcaaagga tagaagtggg actacggcct gttgatgaac ccctgggtca tagttggatg
    27421 aggggcaatc agccggcggc ggtgctggcg gtgggcgcgg cggtcagccg ccgccggtcg
    27481 gcggggcggg gggcggggtt tcctcttcgt cggccaggcc cggaacgggt tcggcctcca
    27541 gtttcttgat ccccagatcg taacctttag tcgtacggac gacgccgaag acgacccgca
    27601 cgacgcgcga ggtcttcggg tcgagcccct gcttctccag ctcggcctcc tccagttgca
    27661 tcatccgcgc gatggccggc ggcccgtaat tgaagacctg aaagacgccg ctgccgctgc
    27721 gcttgacgaa ctggccttcg ttggtctggg cgcggctgag aaagccgggg ttgggatcgt
    27781 tcccgttcag gttcatcagc atgatccgga tgccgtaacg ccccagcact tcctgcgaca
    27841 cattgggcgg cacgccggtg atgtcgattt cgcggatcgc cccctcgttg agcccctcgg
    27901 tgcggaacag cgtctggtcg gcgctgagct tgctctggag catggccagt cggcggtcga
    27961 tctggcgcga gcggcccttg cgcagttcct cgggcaaatc cagttgcggg acgggaacgg
    28021 acgggtcggg gcgctcctcg tccgcgggct cgggctgcgc cgcggcggcg gcggcatcat
    28081 ccggcggcga ttcctcggcg ggttcttcga acgcttcatc cggcgccagg gtctccgcga
    28141 cgggcgcgtc ggttaccggc caggattcgg tttcgatggg tatttcgtaa tctgcggacg
    28201 gctcgatcag ccagtcggtc ggctcaatga cgggcgtcgc ggccggcgtc ggcgacggcg
    28261 tgggttcggg ggtcggctcg ggcgtcggct cgggggtctg aacgacgacc ggctcgggtg
    28321 tcggtgatgc cgtcggtgtc ggcgtcgccg gccgcccgct ccaggcaggc gggcttatcg
    28381 tgacaaacat aggcgccccg gccggccagc ttgccggtgg gatccagcgt gacccggccc
    28441 tccttggcgg tgcgcaccac ccggaccagc tccttcttgg gccggcgttc ccggcagccc
    28501 atgcacatgc gcacggggat tttgcgcaca gccatattat gcc
//
LOCUS       DAOPCO010000023        28186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1838_length_28186_cov_6.291387, whole genome shotgun sequence.
ACCESSION   DAOPCO010000023 DAOPCO010000000
VERSION     DAOPCO010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28186
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_28186_cov_6.291387"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLS90_03300"
     CDS             <1..608
                     /locus_tag="PLS90_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01462.1"
                     /translation="GFALADAVLAALQTGDEPPAAPLELDYALGQIDGLPLRTFPTVE
                     EALEHHRRAAAHHARLAAEGAERGRVRTAECAVFGAEERITLARAQADGRLARAVREV
                     LPAPLQVLRVGPRLIVGWPGEVFVEHSLAFQRRCPMEAHVIELANGELEGYVVTEEAD
                     RLQTYESQNSLFPPVAGEMLIDATLALVADLLRGDSPAGET"
     gene            complement(882..2888)
                     /locus_tag="PLS90_03305"
     CDS             complement(882..2888)
                     /locus_tag="PLS90_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK01463.1"
                     /translation="MAGLLLVAAAIWLSLAPDPALAAQDAATASRSASPEQFRRELEG
                     PILSIPTPFTRDGAVDYDGVREMIRRARAHGIRIFNLTGGNSRYDMLAYDEIRELTRV
                     MQEAAGADALTIGCAHEFWTARLLDYARYARSLGVDAIQVTAPSYLGAEELPDYFRTL
                     GREVDLAIVLGNQDLPGAVMEQLLEIPQLVAMKEDSTLTAYIDNLRRYGDRLNIYSGG
                     SLARFLVGRPYGARAYFCSYTTFAPQISARFWEAVRAGDEAEAFRLAERYDLPWIDRF
                     SHPWWRATLEYFGVAERWLRPPMRSFTDEELRQVETFFQNLGLHPDGEQPRRASGEPA
                     PANPASAEDMMDWSWNVLSPLPHGVGAAHLLHDEHGVYLVGGDAWTTSSGTERKVNLD
                     TIYRLRLSSGRPEEARWDEAGRLPVPIANGAIGLWRGRLFLMGGKSGEEMNRELWSGS
                     LAGQDWTRLGAWEQPTWGNVAVQHEQNLYSLGGWTTPESDERLSAAVWRWNLEEPEAP
                     PVQATSLPGAPRALFAAAVFDGELYVFGGWTMSGGEVRFLRDAHRFRPATAEWEALPD
                     LPLALEAHRAIAIEPAGILLVGGYSYLSADDPQPDKGSIRDDLWLYDPRRRSYERVGQ
                     LPAPVCDMALARLDHALLVVGGEDRGRHRTAQTLLAPLPAQWQD"
     gene            3382..3813
                     /locus_tag="PLS90_03310"
     CDS             3382..3813
                     /locus_tag="PLS90_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01464.1"
                     /translation="MNRAIARFRRQGFTLIELLIVVAIIAILAAIAVPNFLEAQTRAK
                     VSRVRSDQRTIGLGLESYFVDYSKYPNSSLFIGESNQTVPWQIQPVFRWATVGGLIEL
                     TTPISYLTRYPPDVFDIKAPWTSSPVQNRETMNRLDRPYDS"
     gene            3810..4040
                     /locus_tag="PLS90_03315"
     CDS             3810..4040
                     /locus_tag="PLS90_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01465.1"
                     /translation="MNIRTYAEAYSVNPNLIGPFYANLYHAGVRYFIRSWGPYGQVVE
                     AEYDPTNGTVSGGGINYYGPQISQQAKVEASS"
     gene            4128..5999
                     /locus_tag="PLS90_03320"
     CDS             4128..5999
                     /locus_tag="PLS90_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01466.1"
                     /translation="MTQRLARRRPATCLLFLAVALTATLGRAQVQPWGGTQVMEWERA
                     AEARIVGDMSRCEPAAALSPDPAPGKWRLIPYETVTGVAGTMIWASPTEGFPPPDAEE
                     LGTLPGVALARGGVEPVALPLETEGWHALFVALFSTGEATTRVYMKLDGDPAPVGRTQ
                     FGTAHRANLEEVFFKVAQLKPGDRIHFSQQNNGYPDAAGVAYVKLIPLSEAEIARQEA
                     DRESREHLRLAASHDGFSTLFHRAMTTPEELLSDIELFRDTDFGTVILHNMGGDKVNY
                     ESAVGHMAGSTLSDFARIGDWNFARATAALRRQGINPMQVMIDGARAMGLRVYVAIRP
                     AGWSFWEPLTRFWETPFYRDHPEWRCVDRDGTPVTRMSWAVPEVRAHMIDLLMESVRL
                     GADGAQLVFNRGCPMVLYEPPAREIFERRHGVDPRTLEETDPRIRQWWADIVTTFFQE
                     LRAALDEEAERRGDGRRLDLTATVHGIEADNLLYGIDLRRLVSEGLVDGLYIYPWDFG
                     RAAAAYGEIELEFFRDICQPAGIPFYPALGLHLFNAREYAQRAREMYEAGAAGIFVWD
                     AAITDLPKWGVIRRLGHVEELPERIERLAPDPPHEYIQFHRLGPNIMDSRYPPWWGG"
     gene            6047..7588
                     /locus_tag="PLS90_03325"
     CDS             6047..7588
                     /locus_tag="PLS90_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01467.1"
                     /translation="MLFGLQALDLVVIAGYFLAVAAVSWYVSRGGNTAEEFLLAGRRA
                     GRIMIAISTLACSFSAISFVGITGWVRDQGMVGFIGDSGILIGSLFIAAFLIPLYYRR
                     GVVSIYETLETRYHRSVRLLVAALSQLLRVSWLAGLLYAPALMFETITGISVGYWICF
                     MALIGIVSAISGGLKSVMYVDLIQFGVMIVAIVYLILLSCSLLDWDLGMIWRTASEAG
                     VTNFIEIDFNPFEVSTLYYVFGVSLITVSGFGADQMGAQRFLSARDVRQSQQSVLIFP
                     LITISFVFLFYMGAMLTSVYFHVRPELAAGLEKADQYLPQFIKVAMPAGFVGLMIAAV
                     MAASVSSFAGGVISLVTITMEDYLKAFYQGKGERRLLRDTRLVTLAWCLVGVVLSFYV
                     LRLGTVIEWILMVGGYFGGIILAVFLLALYTRTTTTRGVLAGAICGLVITAVAARYNF
                     GTPAEPLRLNLFLYPPIGLASTWVLAFLFSRLLSPGSAARPAAEIQDAADAPSSETAL
                     SRSEP"
     gene            7588..8805
                     /locus_tag="PLS90_03330"
     CDS             7588..8805
                     /locus_tag="PLS90_03330"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK01468.1"
                     /translation="MSKPFRLRVMCLALAWLTAGPRPALSAEDDKPWVAPGFTDLELD
                     HAGPFVTLDDGSLLQFATTGPFGSFTPSVLRVSTDRGKTWSEGTPILRDGPAPATLVD
                     LALRTSDGTLVVIYCDYQNEKWNWDEERGEATIAQLDTWSARSLDNGKTWIDRQLISP
                     GWNGAPIDIIETAEGAIVTPVMLLIEDPGRHRHGFRNFVSTDQGQTWRKGNIIDLGGA
                     GHHDGAIEPTLAQLRDGRIWVLIRTNLGRFWSAWSEDGGLNLREMAPSPIEASSSPAF
                     LKRLASGRLLLVWNPAQPPDGTPPPMHGGLERPGEAQYSETEVSWYREGAYIALSDDD
                     GQTWSQPLLFVEHKGRRVSYPYVFEPWPGEIWVWLRQSGNPGETSSLTVSVMERDLAR
                     MVGMAPEEAETEQ"
     gene            9005..9433
                     /locus_tag="PLS90_03335"
     CDS             9005..9433
                     /locus_tag="PLS90_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01469.1"
                     /translation="MSSGDFYFAINATFRFIHDRFGMDGLRAYWRELGRDYFRPLSER
                     FRDGGLPAVEAYWRDFFRQQPCGAAEVIREPEQVRIEVRDCPALRWLRDHNRDVFEHY
                     CDHCRYVSEEIADQAGMRFELEGGGSQCRQRFISGRDAQP"
     gene            9430..9966
                     /locus_tag="PLS90_03340"
     CDS             9430..9966
                     /locus_tag="PLS90_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01470.1"
                     /translation="MIGVHDFCGHYDWTFEYLRRLGGEELVARYWREAIGFDSQRHAL
                     QLFLAKGIEGMAEYWGHTLTLEEAGYRLSRNQDVFRLDMHACPSKGYLIEHGLEAYPD
                     YCTHCIGWIGPVAEQAGFQVDHEHNHCGQCWWELHRNAGEEARRDVREQAGADNVEHH
                     PRWPMGTHHRFRNGKPES"
     gene            10114..11235
                     /locus_tag="PLS90_03345"
     CDS             10114..11235
                     /locus_tag="PLS90_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK01471.1"
                     /translation="MANHTASSAQNRKQALYRRIYEELYREISEGKYAGAARLASENE
                     LCRRYQTSRQTVRLAVDGLINKGLVERVPGKGLYLIDQSRQPLRSSRAHILVVSPQYD
                     HQNEGDVLFDAKILQGIRSIFAESSVLELRSYPATANRASDIGRYYSPNRIDGLILIS
                     PPPDLQRRLGEIQVPLVVFGHLLPGVHVPHVRLNMFKMGYDTATTLLGLGHSRWGMLS
                     SSSLTNPGVRRFIEGAENALVDYHQVWNPADRAEFGNLRDCLKKLDNFVDRGVSAIIL
                     DGPDYALSVYHHLQERNLPVPEAVSFLVLEMNRNMTRQPPFFSGFFTDHFAYGQTGAQ
                     VLAGLMSGERTTPGEGQRMVELEFYPGKTLAPAQTAGSA"
     gene            complement(11232..12167)
                     /locus_tag="PLS90_03350"
     CDS             complement(11232..12167)
                     /locus_tag="PLS90_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01472.1"
                     /translation="MSEFRFQPARFIPYRDVEAIDRVLKIPMEKLTEHPNSDFRIEIL
                     PAAEIEFRWITDMFWRIKHAADAGEELTLILPQPYPGYRKLARLINQWRVDCRRLHTF
                     NMDEYADEEGNIAPEDWPQSFGYALRRHLYSQIDRDLRPPADQMIGLTNKNLADYGKM
                     IADRGGADVCYCGPGWTGHLAFIEPDAPEFAAASLEEWKTFGPRICTLSPFTIAQNSL
                     HAAFGASGNLCAVPPKAATIGPAEVIGARHRIDMHAITVGGSPVAWQRVASRLVLHGP
                     VTPQVPESILQTLRTDVWVSEAIAADIVNDPEGGY"
     gene            complement(12173..13387)
                     /locus_tag="PLS90_03355"
     CDS             complement(12173..13387)
                     /locus_tag="PLS90_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01473.1"
                     /translation="MYDLLIEHAQVVLPDRLWTDAAVACAEGRIDAVVSSAEAAGLAA
                     RERLDARGDYLLPGLIDIHINGIHEYLVNDGGADLAGLLRALPRHGVTACLPSVLPPA
                     DRPLKELVAELAGARLPDAGARMLGLFLEGPYLTCTGAARPLAEEVRLNELAELIDAA
                     RPHQAVMAVAPDFPGIGPALEFMRRRGVPMFMTHTRATVEQTRAAIEAGVRHATHLFN
                     VFAPPPPREPGLHPFGAVEAVLADPRVSVALILDGVHVPPVGLQLALRCKPAERVCLI
                     SDANRGAGLPPGRYRSFGYDVEYESPGAPARIARDSPTMAGALAGSGLTLDQALRNAV
                     SMLDLDLPVAAQLVTESPARVLGRDHEMGRIAPGLRADLTLLDQRLVPRRTWIGGRLA
                     DCSDASSVEQGA"
     gene            complement(13462..15264)
                     /locus_tag="PLS90_03360"
     CDS             complement(13462..15264)
                     /locus_tag="PLS90_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01474.1"
                     /translation="MRWLSLPLLMLVGIASLHAAEPPEGIQPWNGAAVTDWTNHEQPI
                     VITDLTQAEPADAFSDDFRRGHWKVIPYEMRPDSGGYKGKAVWCGYEADPPSLRIKLN
                     AEGWHAIFVGFLSLTGAYVKLDQDPAPLWLNNPIRDYYVNSVDTYFKAAELTKDSTLT
                     ISPKRHGSRQPAGITSIMLIPLTEAEVQRLQRDRADTSNRRLVATADGFSSICGNSPR
                     TVEECLAEIEYYRNTDFGTLIYHGGWSGDKTIYPSRVGYLPGLELEDVCQDYHRDFID
                     AVRELADKGINPLKVIIEGAHEMGMKVHVGMRPGGWSYYQPFNGLWDSPFYLNNPQWR
                     CRDHASRDSAEITRMSWAVPEVRRHIIDLLMEQVGFGADGAHIVFNRGFPMVLFEEPF
                     CKLFRDKHGVDPNELDENDPRIVEMWSDIITLFFKELRAELDKEEARRDNGQPIEISI
                     MVLGTHEDNYHYGLDIRRLVNEGLIDEIQIYPYGFGNVLPGSLYDEAFFKEVCEPAGV
                     RYYPTVDPPYDVTGQLGRALELYDGGAPGLCYWDAGHVDRPTWAIQSRLGHPEEVRWR
                     REHVNTDDYPESIRFYKWWGDQRMDVRYPPYWGG"
     gene            complement(15366..17114)
                     /locus_tag="PLS90_03365"
     CDS             complement(15366..17114)
                     /locus_tag="PLS90_03365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK01475.1"
                     /translation="MKRLHFRSGLQFAPRTTRAAWLALMLLACARVPAEVMPPTPVGN
                     LPEKPMVAQFSDGTLAGVLLQREGTQQFIAARFSSDNGDTWTSASRLFEIPEAEGLVE
                     GYMIMIDRGDNFHCFYLNNPNKKPAAPGGEAERPPVGSLGQPRLDIWHAVSSDRGATW
                     TTPRCIWEGYTGALNSIIQLKSGRILVPFSLLTDRSWWNRDDGPFNFSFNGQYDCTLV
                     YSDDDGATWHESPARLSVVVPDIVSAYGAVEPVVLELKDGRVWMLIRTQMGRFYESFS
                     EDGVEWSAPRPTQIVSSDSPAGLVRLTDGRIVLFWNKCQRFPYAYGGRHVLHAAISDD
                     EGRTWRGHREVARDPLNDQPPPAGGDFGTAYPFPIATLDGKAIYYTGQGEGRVDVMRL
                     DPDWLLERSAVADYSTGLTEWSTFGTKGVSIETGSSDAPDKHLKITRPDADWAAGAVW
                     NFPLGARGELTFDLLLPEGSRGATILLADHFSPPFDAEDEFYSLFNLNIGADGTVGNS
                     QLAHGNWHTLRMNWDVARRECVVQVDGQEAAVLPLRHEAQGVCYIRFKTAGDDPDPAG
                     LRVGPVKATLAADDRP"
     gene            complement(17463..18404)
                     /locus_tag="PLS90_03370"
     CDS             complement(17463..18404)
                     /locus_tag="PLS90_03370"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK01476.1"
                     /translation="MSQTLKTRQPLTLDEARHHLTGPIASVHTPFLRDGELDEASLAA
                     QIGRYLEAGSRTILMTVGNSHYFCLSDDEIARVTRIVCREAAGRAMVVAADRMHATRR
                     AVEFARFAREEGADLVMCMPPDWNNSCTPATLAQHYATVAAEWPVMIVTNLMGPRGDA
                     FGMEALRLSLDLSARIVAIKDDVCGLFAQKMTVHFRERAAIFAGGLKLNHLNIAPYGA
                     DGYMSTFIGFCPEITRRYWSCIEAGDMAGAWRITETYETPLFELMSSFPGDWNAGWHG
                     CLELFGIGKRWRRPPYYSLNDEEMERLKDFFVSRRLL"
     gene            18947..20317
                     /locus_tag="PLS90_03375"
     CDS             18947..20317
                     /locus_tag="PLS90_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01477.1"
                     /translation="MRDVELALRRGHLLTLDSHRRIFPEGTLLIDQGRLVDVGPDQAL
                     DGTYHARDVVDLAGAVVHPGLVDAHTHVAWHLTRCWKSEEQDPDRLWSELEYPLITHI
                     GPRDEELGTALACAEMALNGTTTFCDTGSCLHLEAAVAGARRIGLRGIMGYLLADVGL
                     DHPRLKLSSRQCAELLERAIVEFPWDEDHLVGCAACLIGMGTASDELLRQARALADDH
                     GVPVVMHESFCALAPHDAQRRYQGRLPVPRLDELGLLDRPTTLVHMNQVTTAELEILR
                     EKRPAVVHCPTAGMIYGLGAARHALFPEMLAAGVPVALGTDSCHWGNNFNLWRSLYLA
                     VALHREARARPSVIADATALEMATRHGARAVGLEHAIGSLEPGKQADLVIHRRDRPEL
                     WPLLFPVRNLVFGCGGATIDAVLVQGRYVVRNGRLTNVDLEDLLAEAQEAALALAHRI
                     GLVTPL"
     gene            complement(20362..22068)
                     /locus_tag="PLS90_03380"
     CDS             complement(20362..22068)
                     /locus_tag="PLS90_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01478.1"
                     /translation="MLDGRGSGVLALLILIVLVAPRVAAESIVFSKHNLSVTGPGAVR
                     ATSEEQICIFCHTPHGGRTEAPLWNRRDSTAAYIPYNSPTLKAAVGQPTGASKLCLSC
                     HDGTVAMGDLVSEETIVQLTGSGVMPPGPGLIGTDLRDDHPISFNYFDSLAQAGGELT
                     APATWDARVKLEEDGELQCTTCHDPHDNEWGQFLVMPNQEAMLCRQCHNQPLFPQTPH
                     AESPQVWNGQGEDPWPHTEFPDVRANACMNCHMSHHAGGFEGLLTRAREEETCFACHN
                     GNVARFNLKAVFQKPYRHPVEAFQGVHEAGESPLEAAGHVECADCHNPHQAARAEAQP
                     PFVMGVLRGVTGVDMNGAPVREAVYEYQICFKCHAGEGTSGFGSIERQINSSNVMQEF
                     NPASPSYHPVVAAGRNPDVPSLISPLTPASLMYCSDCHGNDGAQESVVGSAGPHGSNY
                     EFLLARQYRTGDDVAESPTAYALCYGCHSRESLLADESFSGHRRHIVEERISCSVCHD
                     PHGIDFGEANPMNHAHLINFDTSVVEPDPLTGRLEYRSAGPRMGECYLSCHGVAHSPR
                     SY"
     gene            complement(22221..23240)
                     /locus_tag="PLS90_03385"
     CDS             complement(22221..23240)
                     /locus_tag="PLS90_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HPK01479.1"
                     /translation="MVLVLLALVAACAVQPWSGFPTAVRPELTWPGPPLPARVGFVAE
                     IRGHRDLFEEGGGLRTIGRWLSGPADSGVIRPYAVALHGAGGLLVTDPGRCIVHFFDG
                     PRRRYVALGAELEGGLPSPVGVAALPDGRILVSDSRLGRVLIFDRDGAPQGTFAGPPA
                     LTRPAGLAVHPARGEVLIADVAAHRVRVFDLAGRPLREFGGRGDAPGQFNFPTHIAVG
                     ADGRIAVCDSMNFRVQVFAPDGAPLRTIGELGDAPGQFAKPKGVALDREGRLIVVEGL
                     HDAVQFFSVEGELLLSIGRPGQAPGEFWLPAGVAYDPATDLLFVADSYNARVQVFRML
                     GGAGE"
     gene            complement(23357..25417)
                     /locus_tag="PLS90_03390"
     CDS             complement(23357..25417)
                     /locus_tag="PLS90_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01480.1"
                     /translation="MTVPPRPSPAAGAGLVRLGLLIIGFMLGMAAAHGQTRRSSLDAP
                     IAPLRITERGGELGLRFDYLSETQRRDGGDEVELSNRAFQEYLIQRIGGYVYHPRFLE
                     FRSRFEIGMMQQFIENSGLGGFAGGVEESSRSNSFIGGYDLYLHFLKEHPFSAMVFAG
                     RDRRPVLQLFTDRQIVESERYGVVFNWKKDPFPMDLALTRTKLREFGFDSHSESDNTL
                     LDYVVRHRLGRRMETELRYRLQDYQQSFTAENPLITIERETELQSHDLSLLNTIYLNE
                     DRTSYLSSILRYFTQTGSQELATMQWHERLNLRHTPNFSTYYLASLLENEFSRTSVLT
                     RRLEAGLDHRLYRSLDSHLDIHGRWIEFDESEETEIGVTGRLGYRKQTPWGYLTAGYG
                     RTLDRVTRTGLSDRRPVIDEAVTLDDVAFTFLSQPAIIASSVQVTDATGVIIYLEGFD
                     YELEERGNRLGIRRLATGNIDDGETVLVDYSVEFTSDLEYLADHQIFHVRYDFERFLE
                     GLAVYYRWQDLTARDAPRVDDFSILEYTDWLAGLQYRWRWLTWSEEFERYESNFSSYD
                     QLRSQIEGHHPLGRGIRLGWHAGTLLIDYRDEGLDNPYTNVVYGGLTLDGRIRPSGFW
                     QIEARARKESGLSDEMLVGIAGKVGMIWRKLKLEAGARVEQRQRFESERDRMQVFLQV
                     TREF"
     gene            complement(25493..26329)
                     /locus_tag="PLS90_03395"
     CDS             complement(25493..26329)
                     /locus_tag="PLS90_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPK01481.1"
                     /translation="MDLRARAGLTALLWLGALLPAAAARAVIQPGEVAPSIELQDLND
                     QTLRLDAPGTGVAVLLFTKPGDRHTSAAVAAINGILAQHPEIDRRATRRAILSRLRND
                     DQIAAAGERIGPGWPLLLDRDDAAYGAYRIIATPTLVIVGADGLVSAVHPGYDPGMAQ
                     AVRLALAAALGVELPAAATQAAAPPNMDLQMARRLAARGLWERALEYYDRLKPEDVTD
                     AVRVERAAILLELGRLEDALADLAAVPADSPHAARAEELRRLVTEKQAGDAAPAPPAV
                     TR"
     gene            complement(26829..>28186)
                     /locus_tag="PLS90_03400"
     CDS             complement(26829..>28186)
                     /locus_tag="PLS90_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01482.1"
                     /translation="LRLQLARDFVAPRLALVVAVLGGLAHLGRAQDGAPIQLGPSCVT
                     AECHADINQGQFLHGPVNMGQCQPCHVPVNNQHVFEKPAGGEPVCLLCHETEQPKAVV
                     HAPFAADCAVCHDPHNGGNRYFVKGGLGAESCNQCHSDVSAGLSMLHGPVAVGECLVC
                     HTPHQSEHAHLLVEPRATLCQSCHTDVQAELENAVSVHTPVKQDCAGCHNAHGGNREY
                     FLVAEGQDLCRKCHEPFLKQMSQFKHPHEPMTEGKGCVACHEAHVSSQEQLLKAHNME
                     LCLSCHNETIAGARGMVENIQAQIGNARFLHGPLREDNCIACHQAHGSDHPNILEKAF
                     PDSFYTSFESEKYDLCFACHDKQIILEEKTAATGFRNGEQNLHYLHVNREKGRSCRAC
                     HHEHAANQPNHIREEVPFGRWRMQVEFEKTETGGSCSTGCHVPYKYDRASPVQNVAAR
                     E"
BASE COUNT         4648 a         9757 c         8943 g         4838 t
ORIGIN      
        1 tgggcttcgc gctggccgat gccgtgctgg cggcgctcca gaccggggac gagccgccgg
       61 cggcgccgct ggaactggac tatgcgctgg gccagatcga cggcttgccg ctgcgcacgt
      121 tcccgaccgt cgaggaagcg ctcgaacacc atcggcgggc ggcggcgcat cacgcccggc
      181 tggcggccga aggcgccgag cgcggacggg tgcggacggc cgaatgcgcc gtgttcggcg
      241 ccgaggaacg gatcacgctg gcccgcgcgc aagccgacgg ccgactggcc cgcgcggtgc
      301 gcgaggtgct gccggcgccg ctgcaggtcc tgcgcgtcgg accgcggctg atcgtcggct
      361 ggcccggcga ggtgttcgtc gagcacagcc tggcgttcca gcggcgctgc ccgatggagg
      421 cccacgtgat cgaactggcg aacggcgagc tggagggcta tgtcgtcacg gaggaggcgg
      481 accggctgca gacctacgag agccagaaca gcctgttccc gccggtcgcc ggcgagatgc
      541 tgatcgatgc gacgctggcg ctggtcgccg atctgcttcg cggagattcg cccgcggggg
      601 aaacatgacg cgtgcgagcc agcaagcgtt ccatcttgcg gggacatccg gttgatggat
      661 catgattgaa ccgatcaatc ggttcttcgg gttggcggat cgtcaccgtg ggttgaaatg
      721 gcggccgagc gaatggccgg actccagcga aagcggccgg cgcggttcct gatacagaga
      781 acaggcggcg ccgtctcacg tggagggctt tgattcgatt tcgtgcatcc cgcccgttca
      841 ggccctcgcc gtccttcgtg gtttccaatt atttccggtc attaatcctg ccactgtgcc
      901 ggcaacggcg ccagcagcgt ctgggcggtc cggtggcgcc cgcgatcctc gccgccgacg
      961 accagcagcg catggtccag ccgggccagc gccatgtcgc acaccggcgc ggggagctgc
     1021 cccacgcgct cgtagctccg ccgccgggga tcgtagagcc acagatcgtc gcggatgctc
     1081 cccttgtccg gctggggatc gtccgccgag agatagctgt aacccccgac gagcaggatg
     1141 ccggccggct cgatcgcgat cgcgcggtgc gcctcgagcg ccagcggcag gtcgggcagc
     1201 gcttcccatt cggcagtcgc gggccggaag cgatgcgcat cgcggaggaa ccggacctcg
     1261 ccgccgctca tcgtccagcc gccaaagaca tacagctcgc cgtcgaatac ggccgccgca
     1321 aacagcgcgc gcggcgcgcc cggcagcgac gtcgcctgca ccggcggcgc ctccggctcc
     1381 tccaggttcc agcgccagac ggcggccgac agccgctcgt ccgattccgg cgtcgtccag
     1441 ccgcccagcg aataaagatt ctgttcatgc tgcacggcga cgttgcccca ggtcggctgc
     1501 tcccaggcgc cgagccgcgt ccagtcctga ccggcgagcg agccgctcca caattcccga
     1561 ttcatctcct cgccgctctt gccgcccatc aggaacagcc gcccccgcca gagaccgatg
     1621 gctccgttgg cgatcggcac gggcaagcgc cccgcctcgt cccaccgggc ctcctccgga
     1681 cgccctgacg aaaggcgcag gcggtagatc gtatccagat tcaccttgcg ctccgtgccg
     1741 gacgacgtcg tccaggcgtc gccgccgacc agataaaccc catgctcatc gtgcagcaga
     1801 tgggccgcgc ccactccgtg gggcagcggc gacaagacgt tccacgacca atccatcatg
     1861 tcctcggcgg aagcagggtt cgcgggcgcc ggttctccgg acgcgcgtcg gggctgctca
     1921 ccatcgggat gcaggcccag attttgaaag aaggtttcca cctggcgcag ctcctcgtcg
     1981 gtaaacgacc gcatcggggg ccggagccac cgttcggcga cgccaaagta ttccagcgtg
     2041 gcgcgccacc aggggtggga gaaacggtcg atccacggca ggtcgtagcg ctcggcgagg
     2101 cggaacgctt ccgcctcgtc gccggcgcgc acggcctccc agaaccgcgc cgagatttgc
     2161 ggcgcaaacg tcgtgtagga gcagaaatag gcccgcgcgc cgtagggccg gccgaccagg
     2221 aagcgcgcca ggctgccgcc ggagtagatg ttcaaccggt cgccatagcg ccgcagattg
     2281 tcgatgtagg cggtcagcgt cgagtcctcc ttcatcgcca ccagctgcgg aatttccagc
     2341 agttgttcca tgacagcgcc gggcagatcc tgattgccca ggacgatggc cagatccact
     2401 tcgcgcccca gcgtgcggaa gtagtcgggc aactcctccg cgccgagata ggacggggcc
     2461 gtgacctgaa tcgcgtccac gccgagcgag cgggcataac gggcgtagtc cagcagccgc
     2521 gcggtccaga attcatgcgc gcagccgatc gtcaaggcgt cggcgccggc cgcctcctgc
     2581 atcacgcgcg tcagttcgcg gatctcgtcg taggcgagca tgtcgtaacg gctgttgccg
     2641 ccggtcagat tgaagatgcg gatgccatgg gcgcgggcgc ggcgaatcat ttcgcggacg
     2701 ccgtcgtaat ccaccgcgcc gtcgcgcgtg aacggcgtcg ggatggagag gatcgggcct
     2761 tccagttccc ggcggaactg ctcgggcgag gccgaccggc tcgccgtggc ggcatcctgc
     2821 gccgcgaggg ccggatcggg ggcaaggctc agccagatgg cggcggcaac cagcaacagg
     2881 cccgccatcg catatcgact cgaccggaaa ggaatcatct ggttctaaac cctggtgaag
     2941 gattggaaac accggcgccg gcacaacgcg ccggcggttc gccgcgccgg cgcggttcgt
     3001 ggttcatccg gggcctgacc atctcacggt tcttcaagga ggagttgcgt cctgtcccat
     3061 gatccagggc aggcaacggt ttaacacatg ttcagtgttg gtgatctgtt ggtgaaacat
     3121 gtatatcttc ataccggaga gcctgtcaac tcctgaatca tgctttcgaa acttactttc
     3181 acggcatttt gtacgctcgg ggcggctgtc gctcgtcgcc gcctgcgtca gtccgctcgc
     3241 ccgcccgatg caaacatgaa tttatttttc ggcttgaccg aatataccag tatgtaatac
     3301 gcttgccgcc acttgcttgc ataatatgcc ccgatgttcg gccgcgcgcc cgtaccaccc
     3361 tcgccgtgga ggactctcac catgaatcgg gccatcgctc gctttcggcg tcagggcttc
     3421 accctgatcg aactgctgat cgtcgtcgcg attatcgcca ttctggcggc cattgcggtt
     3481 ccgaactttc tggaggcgca gacccgcgcc aaggtctccc gcgtccggag cgatcaacgg
     3541 acgatcggcc ttggcctcga gtcgtatttc gtcgattaca gcaaatatcc caactcgtcg
     3601 ctgttcatcg gcgagagcaa tcaaaccgtc ccgtggcaga tccagcccgt cttccgctgg
     3661 gccaccgtcg ggggcctgat cgagctgacc actcccatca gctacctgac tcggtatccc
     3721 cccgatgtct tcgacatcaa ggcgccctgg acgagcagcc ccgtccagaa ccgcgagacg
     3781 atgaaccgac ttgaccgccc gtatgactca tgaacattcg aacctacgcg gaagcctaca
     3841 gcgtcaaccc caatctgatc gggccgtttt acgccaacct ctatcatgcc ggcgtccgct
     3901 actttattcg ctcgtggggt ccatacggcc aggtggtcga ggcggaatac gatccgacca
     3961 acgggacggt cagcggcggc gggatcaact actacgggcc gcagatcagt cagcaggcca
     4021 aagtggaagc atcatcatga cttcccaccc agtcggacag ccgatgaact gacccggcgc
     4081 ggcgtgaaac cacggcagga cccttgtttc cacggatgtc agcaacgatg acccagcgac
     4141 tcgcacgacg gcggccggcg acatgcctcc tcttcctggc cgtggccctg acggccaccc
     4201 tcggccgggc gcaggtccag ccatggggcg gaacgcaagt aatggaatgg gagcgcgcgg
     4261 cggaggcgcg catcgtcggc gacatgagcc gctgcgaacc cgccgccgcg ctctcgcccg
     4321 accccgcgcc gggcaagtgg cgcctgattc cttacgaaac cgtgaccggg gtcgccggca
     4381 cgatgatctg ggcctcgccg actgaaggat tcccgccgcc cgacgcggag gagctcggca
     4441 cgctgccggg cgtggcgctg gcgcgcggcg gcgtcgagcc cgtcgcactc cccctggaga
     4501 ccgagggctg gcacgcgctg ttcgtcgcac tgtttagcac gggcgaggcg accacgcggg
     4561 tctacatgaa gctggacggc gacccggccc ccgtcgggcg cacgcagttc ggcacggcgc
     4621 accgggcgaa tctggaggag gtgttcttca aggtcgccca actgaagccg ggggaccgga
     4681 tccacttcag ccagcagaac aacgggtatc ccgacgcggc gggcgtcgcc tatgtgaagc
     4741 tgatcccgct gagcgaggcg gaaatcgccc ggcaggaggc ggaccgggag agccgcgagc
     4801 acctgcgcct ggccgcctcg cacgacggtt tcagcacgct gttccaccgc gcgatgacga
     4861 cgccggagga gttgctcagc gacatcgagc tgttccgcga caccgacttc ggcaccgtga
     4921 tcctgcacaa catgggcggc gacaaggtca actatgagtc cgcggtcggc cacatggccg
     4981 gcagcacgct gagcgacttt gcgcgcatcg gcgactggaa cttcgcccgc gccaccgcgg
     5041 cgctgcggcg ccagggaatc aatccgatgc aggtgatgat cgacggcgcg cgcgcgatgg
     5101 gcctgcgcgt ctacgtcgcg atccgccccg ccggctggtc cttctgggaa ccgttgacgc
     5161 ggttctggga gacgccgttt taccgcgacc accccgagtg gcgctgcgtc gaccgcgacg
     5221 ggacgcccgt cacgcggatg agctgggccg tgcccgaggt ccgcgcgcac atgatcgacc
     5281 tgctgatgga gtcggtgcgg ctgggggccg acggggcgca gctggtcttc aatcgcggct
     5341 gcccgatggt gctctacgag ccgcccgcgc gcgagatctt cgagcgccgg cacggggtcg
     5401 atccgcggac gctcgaggaa accgacccgc ggatccgcca atggtgggcc gatatcgtaa
     5461 caacgttctt ccaggaactg cgcgccgcgc tggatgagga agccgaacgc cgcggcgacg
     5521 gccggcgcct ggacctgacc gccacggtcc atggcattga ggccgacaac ctgctctacg
     5581 gcattgacct gcggcggctg gtaagtgaag gtctggttga cgggttgtac atctacccgt
     5641 gggatttcgg ccgcgcggcg gcggcctatg gcgagatcga gctggagttt ttccgcgaca
     5701 tctgccagcc ggcgggcatc ccgttttacc ccgcgctggg cctgcatctg ttcaatgccc
     5761 gcgaatacgc gcagcgcgcc cgcgagatgt atgaagcggg cgccgccggg atcttcgtct
     5821 gggatgccgc catcaccgac ctccccaaat ggggggtgat ccgccggctg ggccacgtcg
     5881 aggaacttcc cgagcggatc gaacgcctgg cccccgaccc gccgcacgaa tacatccagt
     5941 tccaccggct cggacccaat atcatggatt cgcgctatcc cccctggtgg ggcgggtaaa
     6001 ggccccggcc acggttcgtt cccttatctt gcacaaggat tttcgcatgc ttttcggtct
     6061 gcaagctctc gatctggtcg tcatcgccgg ctatttcctg gccgtcgcgg cggtcagttg
     6121 gtatgtctcg cgcggcggca atacggccga ggaattcctg ctggcgggcc gccgcgccgg
     6181 tcgcatcatg atcgcgatct ccacgctcgc ctgttcgttc tcggcgatct cgtttgtcgg
     6241 catcaccggc tgggtccgcg atcaggggat ggtcggattc atcggcgact ccggcattct
     6301 gatcgggtcc ctgttcatcg cggcgtttct gatcccgctc tactaccgcc gcggggtggt
     6361 gtcgatctac gaaacgctcg agacccgcta ccaccgttcg gtccggctgc tcgtggcggc
     6421 gttgtcgcaa ctgctgcgcg tctcgtggct ggccggcctg ctctacgccc cggcgctgat
     6481 gttcgaaacc atcacgggca tttcggtcgg ctactggatc tgcttcatgg cgctgatcgg
     6541 tatcgtctcg gcgatcagcg gcggcctcaa gagcgtgatg tacgtcgacc tgattcagtt
     6601 cggcgtcatg atcgtggcga tcgtctacct gatcctgctc tcctgctcgc tgctggattg
     6661 ggatctcggt atgatctggc ggacggccag cgaggccggc gtgacgaatt tcatcgagat
     6721 cgacttcaat ccgttcgagg tctccacgct gtattacgtc ttcggcgttt cgttgatcac
     6781 cgtctccggc ttcggcgcgg accagatggg tgcgcagcgt tttctctcgg cgcgcgacgt
     6841 gcgccagagc cagcagtcgg tcctgatctt tcctcttatc acgatcagct tcgtctttct
     6901 gttctacatg ggggcgatgc tcacctcggt ttacttccac gtccgcccgg aactggccgc
     6961 cggcctggag aaagccgatc aatacctgcc gcagttcatc aaggtggcga tgccggcggg
     7021 gtttgtcggc ctgatgatcg cggccgtgat ggcggcctcg gtctccagct tcgccggcgg
     7081 cgtcatctcg ctcgtcacga tcacgatgga ggattacctc aaagcctttt accagggcaa
     7141 gggggagcgc cggctgctgc gcgatacgcg cctggtcacg ctcgcctggt gtctggtcgg
     7201 cgtcgtgctc tcgttttacg tgctgcggct cggcaccgtc atcgaatgga tcctgatggt
     7261 cggcggctac ttcggcggca tcatcctcgc cgtcttcctg ctggcgctct acacgcgcac
     7321 cacgacgacg cggggcgtgc tggccggcgc catctgcggc ctggtgatca ccgccgtggc
     7381 ggcgcgctat aacttcggca cccccgccga gccgctgcga ctgaatctgt tcctctatcc
     7441 gccgatcggg ctggcgtcaa cctgggtgct ggccttcctg ttcagccggt tgctgtcgcc
     7501 ggggagcgcg gcgcgccccg ccgccgagat ccaagacgcg gccgatgccc cctcgtccga
     7561 aacggccctc tcaaggagtg aaccctgatg tccaaaccgt tccgcttgcg agtcatgtgc
     7621 ctggcgctgg cctggctgac cgccggcccc cgaccggcgc tctccgccga ggatgacaag
     7681 ccgtgggtcg cgcccggctt cacggatctg gagctcgatc acgccggtcc cttcgtgacc
     7741 ctggacgacg gcagcctgtt gcagtttgcc acgaccggcc cgttcggcag cttcactccc
     7801 tccgtcctcc gcgtcagcac cgaccggggc aagacctggt ccgaggggac gccgatcctg
     7861 cgcgacgggc ccgcgccggc gacgctggtc gatctggcgc tgcgcacgag cgacggcacg
     7921 ctcgtcgtca tctactgcga ctatcagaac gagaagtgga actgggacga ggagcggggc
     7981 gaggcgacga tcgcgcagct cgatacgtgg tccgcgcgca gcctggacaa cggcaaaacc
     8041 tggatcgacc ggcaactgat ctcgcccggc tggaacggcg cgccgatcga catcatcgag
     8101 acggccgagg gagcgatcgt cacccccgtg atgctgctga tcgaggatcc gggccgccac
     8161 cgccacggtt tccgcaactt cgtctccacc gaccagggcc agacctggcg taaggggaat
     8221 atcatcgatc tgggtggggc gggccaccac gacggcgcga tcgagccgac gctggcccaa
     8281 ctgcgcgacg gtcgcatctg ggtcctgatc cgcaccaacc tgggccggtt ttggagcgcg
     8341 tggtccgagg acggcgggtt gaacctgcgc gagatggcgc ccagcccgat cgaggcgagc
     8401 agttcgccgg cgttcctcaa gcgcctggcc agcgggcggc tgctgctggt gtggaatccg
     8461 gcgcagccgc ccgacggcac ccccccgccg atgcatggcg gcctcgagcg ccccggcgag
     8521 gcgcaatact ccgagaccga agtcagttgg taccgcgagg gggcctacat cgcgctgtcc
     8581 gacgatgacg gacagacctg gagccaaccg ctgctcttcg tcgaacacaa gggacggcgc
     8641 gtcagctacc cctatgtgtt tgaaccgtgg cccggcgaga tctgggtctg gttgcgccaa
     8701 tcgggcaacc ccggtgaaac atcaagcctc accgtgtcgg tcatggaacg cgacctggcg
     8761 cggatggtgg ggatggcgcc ggaagaggcc gagacggagc agtgagaggg gcgggccgtt
     8821 gggggcagtt gcgagggcga ggacgaggga cgagagacgc tacaagggag aaacagatgg
     8881 cgcggggagc agcggcctga aagaataata attagaagcc gacgacttgc gcgtcggcgg
     8941 tcccgatctc gatcacacgg gacgtgaaac aaacgaccga ttattccggg gatcacgcca
     9001 tttcatgtcg tccggcgatt tttacttcgc gatcaacgcc acgttccgct tcattcatga
     9061 ccggttcggt atggacggcc tgcgcgccta ctggcgtgag ctcggtcgcg actatttccg
     9121 cccgctctcc gagcggtttc gcgacggcgg gctcccggcg gtcgaggcct actggcgcga
     9181 cttcttccgg caacagccct gcggcgcggc cgaggtgatc cgcgaaccgg agcaggtgcg
     9241 catcgaggtc cgcgactgcc cggcgctgcg ctggctgcgc gatcacaacc gcgatgtctt
     9301 cgagcactat tgcgatcact gccgctacgt ttccgaggag atcgccgacc aggccggcat
     9361 gcggttcgaa ctggaaggcg gcggcagcca atgccggcaa cgattcatca gcggtcggga
     9421 tgctcaacca tgatcggcgt tcatgatttc tgcggccatt acgactggac gttcgagtac
     9481 ctgcgtcgcc tgggcggcga ggaactggtc gcccgctact ggcgcgaggc gatcggcttc
     9541 gactcgcaac gccacgcgct ccagttgttc ctcgccaagg ggatcgaggg catggccgag
     9601 tattgggggc atacgctgac gctggaggag gccggctatc ggctgtcgcg caatcaggac
     9661 gtctttcgcc tcgacatgca cgcgtgcccg tcaaagggct acctgatcga gcacgggttg
     9721 gaggcctatc ctgactactg cacgcactgc atcggctgga tcggtcccgt cgccgaacag
     9781 gccggctttc aggtcgatca cgaacataac cattgcggcc agtgctggtg ggagctgcac
     9841 cgcaacgccg gcgaggaggc ccggcgcgac gtgcgcgaac aggccggcgc ggacaatgtc
     9901 gagcatcatc cacgctggcc gatggggacg catcaccggt ttcgcaacgg caaaccggaa
     9961 tcctgagcct ccctggtttg catggcatga ccctgcgccc ggcgtccaaa ccagatcttg
    10021 attgcggaca cgtgactact taaaattttc acgtcagttt cgcgactccg gtcgcaatcc
    10081 cgccctgtat ccggcacagg gatcaccagc ataatggcca accacacggc aagttcagcc
    10141 cagaaccgca agcaggccct ctaccggcgc atttacgaag agctttaccg ggaaatcagc
    10201 gaaggcaaat acgcgggcgc agcccggctg gcctcggaaa acgaactatg ccgccgctac
    10261 cagacgtcgc gccagacggt ccgcctcgcc gttgacgggc tgatcaacaa aggcctggtc
    10321 gagcgcgtgc cgggcaaggg cttgtatctg atcgaccaga gccggcagcc gctgcgctcc
    10381 agccgcgcgc atattctggt cgtcagcccg cagtatgatc atcagaacga aggcgacgtc
    10441 ctgttcgacg ccaagatcct gcagggcatc cgcagcattt tcgccgaaag ctcggtgctg
    10501 gaactgcgtt cgtaccccgc gacggcgaac cgggcgagcg atatcggccg ctactattca
    10561 cccaatcgca tcgatggcct gattctgatc agcccgccgc ccgatctgca gcggcgcctg
    10621 ggggagatcc aggtgccgct ggtcgttttc gggcatctcc tgccgggcgt gcacgtcccc
    10681 catgtccggc tcaacatgtt caagatgggc tacgacacgg ccacgacgct cctcggcctg
    10741 ggccattccc gctgggggat gctttcctcc tcgtcgctga ccaatccggg cgtgcggcgg
    10801 tttatcgagg gggcggaaaa cgcgctggtc gattatcacc aggtgtggaa tccggcggat
    10861 cgcgccgaat tcggcaacct gcgcgactgc ctgaaaaagc tcgacaactt cgtcgaccgc
    10921 ggcgtctcgg cgatcatcct ggacgggccg gattacgcct tgtccgtata ccaccacttg
    10981 caggaacgca acttgcccgt gccggaggcc gtgtcgttcc tggtgctcga gatgaaccgc
    11041 aacatgacgc gccagccccc gtttttctcg gggttcttca ccgatcactt cgcctacggc
    11101 cagaccggcg cgcaggtgct ggccgggctg atgtcgggcg agcgcaccac cccgggcgag
    11161 ggccagcgca tggtcgaact ggagttctat ccgggaaaaa cgctcgcccc cgcccagacg
    11221 gccggcagcg cctaataccc cccctccgga tcgttgacga tatcggccgc gatcgcttcg
    11281 gaaacccaga catcggtccg cagcgtctgc aggatcgact cgggcacctg cggcgtcacc
    11341 gggccgtgca gcacgaggcg cgacgccacg cgctgccagg caaccggcga gccgccgacg
    11401 gtgatcgcgt gcatgtcgat ccgatgccgg gcgccgatca cctcggccgg gccgatcgtc
    11461 gccgccttgg gcggaacggc gcacaggttg ccgctggcgc cgaacgccgc atgcagcgag
    11521 ttctgcgcga tcgtgaacgg gctcagcgtg cagatccgcg gcccgaacgt cttccattcc
    11581 tccagcgacg ccgcggcgaa ctccggcgcg tccggctcga tgaacgccag atgccccgtc
    11641 cagcccggcc cgcagtagca gacatccgcc ccgccccggt cggcgatcat cttgccgtaa
    11701 tccgccaggt ttttgttcgt caggccgatc atctgatcgg cgggcgggcg caaatcgcgg
    11761 tcgatctgcg aatagagatg ccgccgcagg gcatagccga aactctgcgg ccagtcctcc
    11821 ggcgcgatgt tgccctcctc gtcggcgtat tcatccatgt tgaacgtgtg gagccgccgg
    11881 caatcgacgc gccactgatt gatcaggcgc gccagcttgc gatagccggg atacggctgc
    11941 ggcaggatca gcgtcagttc ctcgccggcg tcggccgcgt gcttgatccg ccagaacatg
    12001 tcggtgatcc agcggaattc gatttcggcc gccggcagaa tttctattcg aaaatcggaa
    12061 ttcgggtgct ccgtcagctt ttccattgga attttcagga cacggtcaat cgcttccaca
    12121 tcgcgatacg ggatgaagcg ggcgggctga aaacggaatt cactcacggc ggtcaggctc
    12181 cctgttcaac ggatgatgcg tcgctgcaat cggccaggcg tcccccgatc catgtccggc
    12241 gcggaacgag ccgctgatcg agcaacgtca ggtcggcccg caggccgggg gcgatccggc
    12301 ccatttcgtg atcgcgcccc aggacgcgcg cgggcgactc ggtgaccaat tgtgccgcga
    12361 ccggcagatc aagatccagc atgctgacgg cgttgcgcag ggcctggtcc agcgtcaggc
    12421 cgctgcccgc cagtgccccg gccatcgtcg ggctgtcgcg cgcgatccgc gccggcgccc
    12481 cggggctttc atattcgaca tcgtagccga atgatcggta gcgtcccggc ggcagcccgg
    12541 cgccgcgatt cgcatcgctg atcaggcaga cccgctccgc cggtttgcag cgcagcgcga
    12601 gttgcagacc gaccggcggg acatgcacgc cgtccagaat gagggcgacg ctgacgcgcg
    12661 gatcggccag caccgcttcg accgcgccga acggatgcag ccccggctcg cgcggcggcg
    12721 gcggcgcgaa cacgttgaac agatgcgtgg cgtggcgcac gcccgcctcg atcgccgccc
    12781 gcgtctgttc gaccgtcgcc cgggtgtgcg tcatgaacat cgggacgccg cgccggcgca
    12841 tgaattcaag cgccggccca atgccgggaa aatccggcgc gaccgccatc acggcctgat
    12901 gcggccgcgc cgcgtcgatc aactccgcca gctcgttcag gcgcacctcc tccgccagcg
    12961 gccgtgccgc ccccgtgcag gtcagatacg gcccttcgag gaataggccg agcatgcgcg
    13021 cgccggcgtc gggcaggcgc gccccggcca gctccgcgac cagttccttg agcggccggt
    13081 cggccggcgg cagcacggac ggcaggcacg ccgtcacgcc gtggcgcggg agcgcccgca
    13141 acagccccgc cagatccgcg ccgccgtcgt tgaccaggta ttcgtggatg ccgttgatgt
    13201 gaatgtcgat caggccgggc aataagtaat cgccgcgcgc gtcaaggcgt tcccgcgccg
    13261 ccaggccggc cgcctcggcg gatgaaacga cggcgtcaat ccgcccctcc gcgcaggcaa
    13321 ccgcggcgtc cgtccacagc cggtccggca ggacgacttg cgcatgctca atcaggagat
    13381 cgtacaccgc tgctgctgcc tttgatgatt cgcggccatg ggtcgcgcgc tccagcgcgg
    13441 gcatcgccgc cggcgcgccg ctcacccgcc ccagtaaggc ggataacgca catccatgcg
    13501 ctggtcgccc caccacttgt aaaatcgaat gctttccgga tagtcgtcgg tattgacatg
    13561 ctcgcggcgc cagcgcacct cctcggggtg cccgagccgc gattgaatcg cccacgtcgg
    13621 ccgatcgaca tgccccgcat cccagtagca caatcccggg gcgccgccgt catacagctc
    13681 aagcgccctg cccagttgcc cggttacatc gtaaggggga tcgaccgtcg gataataccg
    13741 aacacccgcc ggttcgcaca cttccttgaa gaaggcttca tcgtacagac tgcccggcag
    13801 gacgttgcca aaaccgtagg gataaatttg aatctcgtcg atcagcccct cattgaccaa
    13861 ccggcgaata tcgaggccgt aatgataatt gtcctcgtgc gttcccagca ccatgatgct
    13921 gatctcgatc ggctggccat tgtcgcggcg cgcctcctcc ttgtccagct ccgcgcgcag
    13981 ctccttgaag aagagggtaa tgatgtcgga ccacatttca acgatccgcg gatcgttttc
    14041 atccagttca ttggggtcga cgccgtgctt gtcgcggaac agtttgcaga acggttcctc
    14101 gaacagcacc atcgggaagc cgcggttgaa cacgatgtgc gcgccgtcgg ccccgaatcc
    14161 aacctgctcc atcagcagat cgatgatgtg ccggcgcacc tcgggcaccg cccagctcat
    14221 ccgcgtgatc tccgccgaat cgcggctggc gtgatcgcgg caacgccact gcggattgtt
    14281 caggtagaac ggcgaatccc agagaccgtt gaagggctgg taatacgacc atccgcccgg
    14341 gcgcatgccg acgtgcacct tcatccccat ctcgtgcgcc ccttcgatga tgaccttcag
    14401 cgggttgatc cccttgtcgg ccagttcccg gacggcatcg atgaaatcgc gatggtaatc
    14461 ctggcagacg tcctccagct ccagccccgg aaggtagccg acgcgcgagg gatagatcgt
    14521 cttgtcgccg ctccagccgc catgatagat cagcgtgccg aaatcagtat tgcggtaata
    14581 ttcgatttcc gctaggcatt cctccacggt ccgcgggctg ttgccgcaga tcgagctgaa
    14641 cccgtccgcc gtcgcgacca gccgccggtt cgacgtatcc gcccggtcgc gctgcagccg
    14701 ctgcacttcg gcctccgtca gcgggatcag catgatcgac gtgatgccgg cgggctgccg
    14761 cgagccgtgg cgcttgggcg agattgtcag cgtgctgtcc ttcgtcaatt cggccgcctt
    14821 gaaatacgta tccaccgaat tgacgtagta atcccggatc ggattgttca gccacagcgg
    14881 ggccgggtcc tgatccagtt tgacgtaggc gccggtcaag gagaggaacc ccacaaagat
    14941 cgcgtgccac ccttccgcat tcagcttgat cctcagcgac gggggatcgg cttcgtagcc
    15001 gcaccagacc gccttgccct tgtagccgcc gctgtcgggc cgcatttcat acgggatgac
    15061 cttccagtgt ccgcgccgga agtcgtcgga gaacgcatcg gccggctccg cctgcgtcag
    15121 atcggtaatg acaataggtt gctcatggtt cgtccagtcg gtcacggccg cgccgttcca
    15181 gggctggatt ccctcgggcg gctcggccgc gtggagcgac gcaatgccga ccagcatgag
    15241 caagggtaat gatagccacc gcatgggact ttcctttccc gccgactgga ttcacccggc
    15301 acgcctcgtg gaccaccccg gccacgccgc gcgcgagcgt tttacgattg gaattcgccg
    15361 ccgtgtcagg ggcggtcgtc tgccgcaagc gtcgccttca ccggaccgac gcgcaggccc
    15421 gccggatcgg ggtcgtcgcc ggccgtcttg aatcggatgt aacagacgcc ctgcgcctcg
    15481 tggcgcaacg gcagcaccgc cgcctcctgc ccatccacct gcacgacgca ttcacgccgc
    15541 gccacatccc agttcattcg aagggtatgc cagttcccgt gcgccaattg ggaattgccg
    15601 acggttccat cggcgccgat attcaaattg aacagggaat agaattcatc ttctgcatcg
    15661 aaggggggag agaaatggtc ggccagcagg atggtcgccc cccgcgaccc ctcgggaagg
    15721 agcaggtcga acgtcaattc cccccgcgcg ccgaggggga aattccagac cgccccggcc
    15781 gcccagtcgg cgtccggccg ggtaattttc aagtgcttgt ctggcgcgtc ggacgaccct
    15841 gtttcgatgg aaacgccctt cgtcccaaat gtcgaccact ccgtcagtcc cgtggagtag
    15901 tccgccaccg cggatcgctc cagcagccaa tccggatcca gccgcatcac gtcgacgcgc
    15961 ccttcgccct gacccgtgta atagattgcc ttgccgtcca gcgtggcgat cgggaagggg
    16021 taggccgtgc cgaaatcgcc gccggccggc ggcggctgat cgttgagcgg atcgcgcgcg
    16081 acttcacggt gaccccgcca cgtccgcccc tcatcgtcgg agatcgccgc gtgcagcacg
    16141 tgccggccgc cgtaggcata cggaaaacgc tggcacttgt tccagaacag gacgatacgc
    16201 ccgtcggtca ggcgcaccag gcccgcgggc gaatccgacg acacgatctg cgtcggccgc
    16261 ggcgcgctcc attccacgcc gtcttccgaa aacgattcat aaaagcggcc catctgcgtg
    16321 cggatcagca tccagacccg gccgtccttc agttccagca ccaccggttc gaccgcgccg
    16381 taagccgaaa cgatgtccgg cacgacgacg ctcaggcgcg cgggcgactc gtgccacgtc
    16441 gcgccgtcgt cgtcgctgta aaccagcgtg cagtcatact ggccattgaa cgagaaattg
    16501 aacgggccgt cgtcgcggtt ccaccaggaa cggtccgtga gcagggagaa cggaaccaga
    16561 atccggcctg acttaagctg aatgatcgaa ttgagcgcgc cggtgtagcc ctcccagatg
    16621 cagcgcggcg tcgtccacgt cgcgccgcga tcgctcgaca cggcatgcca gatatccagc
    16681 cgcggctggc cgagcgagcc gacaggcggc cgctccgctt caccgccggg ggccgccggc
    16741 tttttgttcg gattattcag ataaaagcaa tgaaaattgt ccccccgatc gatcatgatc
    16801 atgtagcctt cgaccaggcc ttccgcctcg gggatctcga acagacgcga ggccgaggtc
    16861 caggtgtcgc cgttgtcgct ggaaaaccgg gcggcgatga actgttgcgt tccctcgcgc
    16921 tgcagcagga cccctgccag cgtgccgtcg gaaaactgcg cgaccatcgg cttttcgggc
    16981 aaattgccga ccggcgtcgg cggcatcact tcggcgggca ccctggcgca cgccagcagc
    17041 atcagggcga gccaagccgc ccgcgttgtc ctcggcgcaa actggagtcc tgagcggaaa
    17101 tgcaaccgtt tcacggcctg ttctccttaa cttaataaga tccgttcctc atccgaaaat
    17161 cgcccttcgt gctcgtgctc gcctccaaag gcccttccaa aattcacccc tcgtcctcgc
    17221 ctctcgaaag ccgatcaatt catatcgtgc tcattcatat ctttcgtgat cccttgtagc
    17281 tgaccgccgc attcttaatt ccggcagatt cccttctcgt gagccgcccc agcggggcgg
    17341 catttgaata gccgcaggtg gaacacagcg aaacctgggg ccactgtcgc aatacatccc
    17401 cgtccccgaa gggggcgaat cccagcgaaa caggagccgg cgctcaaatc gacgcggccc
    17461 gcctaaagca accggcgcga cacaaaaaag tccttcagtc gctccatttc ctcatcgttc
    17521 aatgaataat acggcggccg gcgccagcgc ttgccaatcc cgaacagctc caggcagccg
    17581 tgccagccgg cgttccagtc gcccggaaag ctcgacatca gctcgaacag cggggtttca
    17641 taggtttcgg tgattcgcca ggcgccggcc atgtcgcccg cctcgatgca gctccagtag
    17701 cggcgcgtga tctcggggca aaatccaatg aacgtggaca tgtagccgtc cgcgccatag
    17761 ggggcaatat tcagatggtt caatttcagc ccgccggcga agatcgccgc gcgctcgcga
    17821 aaatggaccg tcattttctg cgcgaacagg ccgcagacgt cgtccttgat cgccacgatc
    17881 cgcgcggata aatccaatga caatcgcagc gcctccatgc cgaaggcgtc gccgcgcgga
    17941 cccatcaggt tcgtcacgat catgaccggc cattccgccg ccaccgtggc atagtgctgg
    18001 gcgagcgtgg cgggcgtgca ggagttgttc cagtcgggcg gcatgcacat gaccaggtcg
    18061 gcgccttcct cgcgcgcgaa gcgggcgaat tcgacggccc gccgcgtcgc gtgcatccgg
    18121 tcggccgcca ccaccatcgc ccggcccgcc gcctcccggc agacgatccg cgtcacgcgc
    18181 gcgatttcgt cgtcggacag gcaaaaataa tgactgttgc cgaccgtcat cagaattgtg
    18241 cggctgccgg cctccagata ccggccgatc tgcgcagcca gcgacgcctc atccagctcg
    18301 ccgtcgcgca ggaacggcgt gtggaccgag gcgatcggtc cggtcaggtg gtgtcgcgct
    18361 tcgtccagcg tcagcggctg gcgcgtcttc agggtctggc tcatggcgca ggcggttctt
    18421 tctagccgaa ggcatgctga tccattgtcc cgattctcgc tccgtcgagc gaccgccgac
    18481 ggcgcggcgt aatccagttc ccgcccggtc agagtgcgct ccggcgcggc ggggccggcg
    18541 acacggggcc gggttcgccc aggcgcgaac ggatcggccg gccttgcact tcaaaactac
    18601 ctggtcggaa aaccagcacg ccggtaaaat aagcaggcat atactagttc ctcgagactg
    18661 acatacatgt agcacctcgg ccggggaccg tcaaccccaa ccatgcgcgg gcctgtccag
    18721 ccggcgaccc cgtcaagtcc cctattttta aaactatcag gcgccgaccg cgccggcggt
    18781 gttcggctcc ggctctcgtc cctcgtcctc ggctctcgcg gccccatcat acggtcattc
    18841 atggtgaacc ccagctttca cctcacgcgg aagtacatgt ttgttttgac acgtctccgc
    18901 ccgccccgct aagctacgat cccagccagc gaggtctccg tcaatcatgc gcgatgtcga
    18961 actggccctg cgccggggtc acctgctgac cctggattcc caccgtcgga tttttcccga
    19021 gggcacgctc ctcatcgacc agggccgcct cgtcgacgtg gggcccgatc aggcgctcga
    19081 tggaacctat cacgcccgcg acgtcgtcga tctggccggc gccgtcgttc accccggcct
    19141 cgtcgatgcc cacacgcacg tggcgtggca cctgacccgc tgctggaaga gcgaggagca
    19201 ggaccccgac cggctctggt ccgaactgga atatccgctc attacgcaca tcgggccgcg
    19261 cgacgaagag ctgggaacgg cgctcgcctg cgccgagatg gcgctcaatg gcacgacgac
    19321 gttctgcgac actggcagtt gcctgcacct ggaggccgcc gtcgccggcg cgcgccggat
    19381 cggcctccgc ggcatcatgg gctacctgct ggccgacgtc ggcctcgatc acccgcgcct
    19441 gaagctgtcc agccgccagt gcgccgagct gctcgagcgc gccatcgtcg agttcccgtg
    19501 ggacgaagac cacctggtcg gctgcgccgc gtgcctcatc ggcatgggaa cggcctcgga
    19561 cgagctgctc cgccaggccc gcgccctcgc cgacgaccac ggcgtgcctg tcgtcatgca
    19621 tgaatcgttc tgcgcgctgg ccccccacga tgcccagcga cgctatcagg gccggctgcc
    19681 cgttccccgg ctggacgagc tggggctgct ggaccgcccg acgacgctgg tccacatgaa
    19741 ccaggtgacc actgccgaac tggagatcct gcgcgaaaag cggcccgcgg ttgtccactg
    19801 cccgacggcc ggcatgatct atggcctggg cgccgcgcgc cacgcgctct tccccgagat
    19861 gctggcggca ggcgtcccag tcgcgctcgg caccgactcg tgccactggg gcaacaactt
    19921 caacctgtgg cgctcgcttt acctggccgt cgcgctgcat cgcgaggcgc gcgcccggcc
    19981 ctccgtcatc gccgacgcga cggccctgga gatggcgacc cgtcacggcg cgcgcgccgt
    20041 cggcctcgag cacgccatcg gctcgctcga gcccggcaaa caggccgatc tggtgatcca
    20101 tcgccgcgac cggccggagc tctggccgct cctcttcccc gtgcgcaacc tcgtcttcgg
    20161 atgcggcggc gcgaccatcg acgccgtcct cgtccagggt cgctacgtcg tccgcaacgg
    20221 ccgcctgacg aatgtcgatc tggaggatct gctggccgag gcgcaagagg cggccctggc
    20281 cctcgcccac cgcatcggcc tggtcacgcc cctctagatc gtctctcgtc cctcgtccga
    20341 gcagatcctt cccccttcca cctaatagct ccgcgggctg tgcgccacgc cgtggcagct
    20401 cagatagcat tcgcccatcc gcggtcccgc ggaacggtac tcgagccggc ccgtcagcgg
    20461 atcgggttca accaccgacg tatcgaagtt gatcaggtgc gcgtggttca tcgggttggc
    20521 ctcgccgaaa tcgatcccgt gcggatcgtg gcacaccgaa caggagatcc gttcctcgac
    20581 gatgtgccgc cggtggccgc tgaagctctc gtcggccagc aggctttccc ggctgtggca
    20641 gccatagcac agcgcgtagg ccgtggggct ttccgcgaca tcgtcccccg tgcggtactg
    20701 ccgcgcgagc aggaattcgt aattggaccc gtgcggcccg gccgagccga ccacgctctc
    20761 ctgcgccccg tcgttgccgt ggcagtcgct gcagtacatc agcgaggccg gcgtcagcgg
    20821 gctgatcagg ctgggcacgt cggggttgcg gccggcggcc acgaccgggt ggtacgaggg
    20881 gctggccggg ttgaattcct gcatcacgtt ggaggaattg atttggcgct cgatcgagcc
    20941 gaagccgctc gtcccctcgc cggcgtggca cttgaagcag atctgatact catagacggc
    21001 ctcccgcacc ggcgcgccgt tcatgtccac gccggtcacg ccgcgcagca cccccatgac
    21061 gaacggcggc tgggcctccg cccgcgccgc ctggtgcgga ttgtggcagt ccgcgcattc
    21121 gacgtggccg gcggcctcca gcgggctttc ccccgcctcg tgaactccct ggaaggcctc
    21181 caccggatgc cggtagggct tctggaacac ggccttcagg ttgaaccggg cgacgttgcc
    21241 gttgtggcag gcgaagcacg tctcctcctc gcgggcgcgc gtcagcagcc cctcgaaccc
    21301 gcccgcgtgg tgcgacatgt ggcagttcat acaggcgttg gcgcgcacgt ccggaaactc
    21361 ggtgtgcggc cacggatcct cgccctgccc gttccagacc tggggcgatt cggcgtgcgg
    21421 cgtctgcggg aacagcggct ggttgtggca ctgccggcag agcatcgctt cctggttcgg
    21481 catcaccagg aactggcccc actcgttgtc atggggatcg tggcaggtcg tgcactgcag
    21541 ctcgccgtcc tcctccagtt tgacgcgcgc atcccacgtc gccggcgccg tcagctcgcc
    21601 ccccgcctgc gcgagtgaat cgaaatagtt gaacgagatc ggatgatcgt cgcgcagatc
    21661 ggtcccgatc agccccggcc ccggcggcat cacgcccgaa ccggtcagct ggacgatcgt
    21721 ctcttcgctg accaggtcgc ccatcgcgac cgtcccgtcg tggcagctca ggcacagttt
    21781 gctcgcgccg gtcggctggc cgaccgcggc tttcaatgtg gggctgttgt aggggatgta
    21841 ggcggcggtc gaatcgcgcc ggttccacag cggcgcctcg gtgcgcccgc cgtgcggcgt
    21901 gtggcagaag atgcagatct gttcctcgga ggtcgcgcgc acggcccccg gccccgtcac
    21961 cgatagattg tgtttggaaa agacaatgct ctcggccgcg acccgcggcg caaccagcac
    22021 gatcaggatc aggagcgcga gcacgccgct gccgcgtcca tcaagcaatc cgtcgtccct
    22081 cgtcctcgta atcgccccca gaggcctgct ccagaatccg gctctcgttg tcgctcgccg
    22141 gttcatgtcg gtctcgtctc cgcaatcggt cagcctcatg acccatccgc cccttcgctt
    22201 ggccctggcg cagccctgct tcattccccc gcgcccccga gcatcctgaa cacctggact
    22261 cgcgcattgt agctgtccgc cacgaacagc agatccgtgg cgggatcgta cgcgacgccg
    22321 gccggcagcc agaattcgcc cggcgcctgg ccgggccggc cgatgctgag cagcagttcg
    22381 ccttcgacgg agaagaactg cacggcgtcg tgcaaccctt cgacgacgat cagccgaccc
    22441 tcgcggtcga gcgccacccc cttgggcttg gcgaactgcc ccggcgcgtc gcccagctcg
    22501 ccgatcgtcc gcaacggcgc gccgtcgggc gcgaacacct gcacgcggaa gttcatcgag
    22561 tcgcagacgg cgatccggcc gtccgcgccg acggcaatgt gcgtcgggaa attgaactgg
    22621 cccggcgcgt cgccgcgccc gccgaactcg cgcagcggac ggcccgccag gtcgaaaacg
    22681 cggacccggt gcgccgccac gtcggcgatc aacacctcgc cgcgcgccgg atggacggcg
    22741 aggccggcgg gccgcgtcag cgcggggggg ccggcaaacg tcccttgcgg cgcgccgtcg
    22801 cggtcgaaga tcagcacgcg gccgagccgc gaatcgctga cgaggatccg gccgtcgggc
    22861 agcgccgcga cccccaccgg cgagggcagg cccccttcca gctccgcccc cagcgccacg
    22921 tagcgccggc gcgggccatc aaaaaagtgg acgatgcagc gccccggatc ggtgacgagc
    22981 agcccgcctg cgccatgcag cgccacggcg tacggccgga tcacgcccga gtcggccggg
    23041 ccgctcagcc agcggccgat cgtccgcagc ccaccccctt cctcgaacag atcgcggtgg
    23101 ccgcggatct cggcgacgaa accgacgcgc gccggcaggg gcggacccgg ccacgtcagc
    23161 tccggccgca ccgccgtcgg aaagcccgac cacggctgaa cggcgcacgc cgcaaccagc
    23221 gcgagcagca cgaggaccat gccgcacgcg cctcgaatcc acggcctgga tgaccctgcc
    23281 acggggtcat gggcatcgcc gcccgccatc cgcgcgccgg atctttcggc ttctccagcc
    23341 atcggccctc gtcccgctag aattcccgcg tcacctgcag gaagacctgc atccggtcgc
    23401 gctcggactc gaagcgctgg cgctgctcga cgcgagcccc cgcctccagc ttcagtttgc
    23461 gccagatcat ccctaccttc ccggcgatcc ccaccagcat ctcatcgctc aagcccgact
    23521 ccttgcgcgc gcgcgcctcg atctgccaga accccgacgg gcggatccgc ccgtccaggg
    23581 tcaggccgcc gtagaccacg ttcgtatagg gattgtccaa tccttcatcc cggtagtcga
    23641 tcagcaacgt gccggcatgc cagcccagcc ggatgccccg gcccagcgga tgatgcccct
    23701 cgatctggct gcgaagctgg tcgtacgaac tgaaattgga ctcgtagcgt tcgaactcct
    23761 cggaccaggt cagccagcgc cagcggtatt gcagcccggc cagccagtcg gtgtattcca
    23821 ggatgctgaa gtcgtccacg cggggcgcat cgcgggcggt caggtcctgc cagcgataat
    23881 aaaccgccaa cccctccagg aatcgctcga agtcgtagcg gacgtggaag atctgatggt
    23941 cggccaggta ctccagatcc gaggtgaact cgacgctgta atcgaccagg acggtttcgc
    24001 cgtcgtcgat attgcctgtc gccagccggc ggattccgag ccggttgccg cgctcttcca
    24061 gctcgtagtc gaacccctcg aggtagatga tcacgccggt ggcgtcggtc acctgcaccg
    24121 agctggcgat gatcgccggc tgcgagagaa acgtgaacgc gacgtcgtcg agcgtcaccg
    24181 cttcatcaat cacggggcgg cggtcgctca ggccggtgcg cgtcacgcgg tccagcgtcc
    24241 ggccgtatcc cgccgtcaga tacccccacg gggtctgctt gcggtagccc agccggccgg
    24301 tgacgccgat ctcggtctcc tccgactcgt cgaattcgat ccagcggccg tgaatgtcca
    24361 gatgcgaatc caggctgcgg tagagccggt gatccaggcc ggcctccagc cgccgcgtca
    24421 gcacgctcgt ccggctgaat tcattctcca gcaggctggc caggtaatac gtgctgaaat
    24481 tgggggtgtg gcgcaggttc agccgctcgt gccactgcat cgtcgccagc tcctggctgc
    24541 cggtctgggt gaagtagcgc aggatgctgc tcaggtagct cgtccggtcc tcgttgaggt
    24601 agatcgtgtt gagcagcgac agatcatgcg actggagttc ggtttcgcgc tcgatcgtaa
    24661 tgagcggatt ttccgccgtg aacgattgct ggtaatcctg caggcgatag cgcagttcgg
    24721 tttccatccg ccgccccagg cggtggcgga cgacgtaatc gagcagcgtg ttgtccgact
    24781 ccgagtggct gtcgaatccg aattcccgga gcttggtccg cgtcagcgcc aggtccatcg
    24841 ggaaggggtc cttcttccag ttgaagacga cgccgtaacg ctccgattcg acgatctgcc
    24901 ggtccgtgaa gagctggagc accggccgac ggtcgcgccc ggcgaagacc atcgccgaaa
    24961 acggatgctc cttcagaaaa tggagataga gatcgtagcc gccgatgaac gaattggagc
    25021 gcgaactttc ctcgaccccg ccggcgaagc cccccaggcc ggagttttcg ataaattgct
    25081 gcatcatccc gatctcgaac cggctgcgaa attcgagaaa ccgcgggtga tacacatacc
    25141 cgccgatgcg ctgaatcagg tactcctgga acgcccggtt gctgagttcc acctcgtcgc
    25201 cgccgtcgcg ccgctgcgtc tcgctcaggt agtcgaaccg cagcccgagt tcgccgccgc
    25261 gttcggtgat gcgcagcggc gcgatcggcg catcgagcga cgagcggcgc gtctggccgt
    25321 gcgccgccgc catccccagc atgaagccaa tgataagcag ccccaggcgg acaagcccgg
    25381 cgccggcggc gggggacggt ctggggggca cggtcatgag cggatgcagt cctcccagca
    25441 cgcccctctc cggccggcgc gtcgggttga atcgcggcgt catcgggtcg tcttagcggg
    25501 tcaccgccgg cggggcgggc gcggcgtccc ccgcctgttt ctcggtcacg agccggcgca
    25561 gctcctccgc ccgcgccgcg tggggcgaat cggcgggaac cgccgccagg tcggccagcg
    25621 cgtcctccag ccggcccagc tccagaagga tcgcggcccg ctcgacccgc accgcgtcgg
    25681 tcacgtcctc gggtttgagc cggtcgtagt actccagcgc gcgctcccac aggccgcgcg
    25741 cggccagccg ccgcgccatc tgcaggtcca tgttgggcgg ggcggccgcc tgcgtcgccg
    25801 ccgccggcag ctccaccccc agcgcggccg ccaacgccag ccgcacggct tgtgccatgc
    25861 ccggatcata cccgggatgc acggcggaca ccaatccatc ggcgcccacg atcaccaagg
    25921 tcggcgtggc gatgatccgg taggcgccgt aggccgcatc gtcgcggtcg agcagcagcg
    25981 gccagcccgg tccgatccgc tccccggcgg cggcgatctg gtcgtcgttg cgcaggcgcg
    26041 agaggatcgc gcgccgcgtc gcgcgccgat cgatctccgg atgctgcgcc aggatgccgt
    26101 tgatcgcggc gaccgcggcc gacgtgtgcc ggtcgcccgg cttcgtgaac agcagcacgg
    26161 ccacccccgt ccccggggcg tcaagccgca gcgtctggtc gttcagatcc tgcagctcga
    26221 tcgacggggc gacctcgccc ggctggatca ctgcccgcgc cgccgccgcc ggcaacaacg
    26281 ctcccaacca cagcaacgcc gtcaggccgg cccgcgcgcg aagatccatc ccgcggcctc
    26341 ggccaccagg aaaggacgca tcggacatca gggacatcag agagggcaag ccgcacctcc
    26401 gcccgcgcgc gcgttggaat gggatcatgc aggttcgaag cgccatccta ttttctttca
    26461 cttgaatcgt ccctcgtccc tcgtcctcgt cctcactcgg aattctgtct ttccccggga
    26521 tcgcttagat cggttctacc gtccctacgg gactccgtcg ctttgggtat cccagttccc
    26581 gccactgaag tggcgggctg aagtctgccg tccctgacgg gactgcccta ctgatcaggt
    26641 ccggctaagg tgtgggttgt tcgaatcggc cgatcacccg ccaatttcct ccctagttgt
    26701 gaatcgcaga ttcatgagca actcgtaatc gcccccgaag gccaactccg aaacccggct
    26761 ctcggccctc tttcctcgtc gtcggctccc gaaatccgtc cgtcctttcc tccgccgtcc
    26821 ccacccgctc actcccgcgc cgccacgttc tgcaccggac ttgcgcgatc gtacttgtag
    26881 ggcacgtggc aaccggtcga gcagctcccc cccgtctccg tcttctcgaa ctccacctgc
    26941 atccgccagc ggccgaacgg cacctcctcg cggatatgat tgggctggtt ggcggcgtgc
    27001 tcatggtggc acgcccggca cgagcggcct ttctcgcgat tcacatgcag gtagtgcagg
    27061 ttctgctcgc cgttgcggaa gccggtggcc gccgtctttt cctccaggat gatctgcttg
    27121 tcatggcacg cgaagcacag gtcatacttc tccgactcga agctggtgta gaagctgtcg
    27181 gggaacgcct tctccaggat gttggggtgg tccgatccgt gcgcctgatg gcaggcgatg
    27241 cagttatcct cgcgcagcgg cccgtgcagg aaccgcgcgt tgccgatctg cgcctggatg
    27301 ttctcgacca tcccccgcgc ccccgcgatc gtctcgttat ggcacgagag gcacaactcc
    27361 atgttgtgcg ccttgagcaa ctgctcctgg ctcgagacgt gcgcctcgtg gcacgccacg
    27421 caccccttcc cctcggtcat cggttcgtgc ggatgcttga actggctcat ctgtttcagg
    27481 aacggctcgt ggcacttgcg gcagagatcc tgcccctcgg ccaccagaaa gtactcgcgg
    27541 ttgccgccgt gcgcgttatg gcagccggcg caatcctgct tgaccggcgt atgcaccgac
    27601 acggcgttct ccagctcggc ctgcacgtcg gtatggcacg actggcagag cgtcgcgcgc
    27661 ggctcgacca gcaggtgcgc gtgctccgac tggtgcggcg tgtggcagac gaggcactcg
    27721 ccaacggcca ccggcccatg cagcatgctc agccccgcgc tcacgtccga atggcactgg
    27781 ttgcagcttt cggcgcccag cccgcccttc acgaaatagc ggttgccgcc gttgtgcgga
    27841 tcgtggcaga cggcgcaatc ggccgcaaac ggcgcgtgca caaccgcctt cggctgctcg
    27901 gtctcgtggc acagcaggca gaccggctcg ccccccgccg gcttctcgaa cacgtgctgg
    27961 ttgttgaccg gcacgtggca cggctggcac tgccccatgt tgaccgggcc gtggagaaac
    28021 tggccctggt tgatgtcggc gtggcactcg gccgtgacgc acgacgggcc gagctggatc
    28081 ggcgctccgt cctgcgcccg gcccagatgc gccagaccgc cgagaaccgc gacaaccagc
    28141 gccagccgcg gcgccacaaa atcgcgcgcc agttgcagcc gcagcg
//
LOCUS       DAOPCO010000024        28177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1839_length_28177_cov_5.724913, whole genome shotgun sequence.
ACCESSION   DAOPCO010000024 DAOPCO010000000
VERSION     DAOPCO010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 28177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28177
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_28177_cov_5.724913"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..671)
                     /locus_tag="PLS90_03405"
     CDS             complement(87..671)
                     /locus_tag="PLS90_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPK01483.1"
                     /translation="MATLANENLAEAAVQVLNQIAPDCVAERIVAPSGEVTLLIDKRY
                     IHDAVKALRDDAGLSLTMLSDVTAVDYLELGREPRFDVVYHLGSLEHAHRLRLRVPVG
                     EDEEECRIDSITDLWKGADFMEREVYDMFGIRFDGHPNLQRILMPDNWEGHPLRKDFP
                     LGGATSFYYKQDSDEYAGEPADLVPRIRVQDGDV"
     gene            complement(802..1407)
                     /locus_tag="PLS90_03410"
     CDS             complement(802..1407)
                     /locus_tag="PLS90_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPK01484.1"
                     /translation="MDIRDLDRRPPEDAGGLVLADPLKRPPLINREALALQDKLRPLE
                     YDNQLVKTLDEVVAWCRKNSIWPVMLGLACCAIEMMGTAASRFDLSRFGMEVFRPSPR
                     QADLMIVAGWVNKKMAPVVRRIYDQMTEPKWVIAMGACASCGGMHNVYSVLQGVDQII
                     PVDVYVPGCPPRPEALIYGILQLQKKVQHSPQRIITPRGEG"
     gene            complement(1398..1775)
                     /locus_tag="PLS90_03415"
     CDS             complement(1398..1775)
                     /locus_tag="PLS90_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HPK01485.1"
                     /translation="MDYLAPYIPILMLLVIALGFGIVTLALSGLIGPRVITFTKLLPY
                     ECGNVPTGEANVRIPIKFYLVALLFLLFDMETVLILAWAIAFRDPTIPNFQPYAFLAM
                     VFFLLILVVGFIYEWRKGALEWT"
     gene            2390..3013
                     /locus_tag="PLS90_03420"
     CDS             2390..3013
                     /locus_tag="PLS90_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HPK01486.1"
                     /translation="MPHPWSAFTLIELLIVVAIIAILAAIAVPNFLEAQVRAKSSRAQ
                     ADIRSVATALEMYRVDYNAYPTMIEPGFPHVYGLTDLKWWYVPDALSTPVAYLGSADL
                     HCPFGGDFKNQRDNFPGEIWRRYSYENIRELEQYAASYPLLQAKYGPQQHALERIGGW
                     RLLCIGPDIEWNPMVQYDPTNGTVSLGNIMRSQKDPVGRNSEPLAVN"
     gene            3254..3922
                     /locus_tag="PLS90_03425"
     CDS             3254..3922
                     /locus_tag="PLS90_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPK01487.1"
                     /translation="MAHNPSTPADRPWPADQLRPCVFLDRDGTVTREAGYINHPLRLE
                     LLAGAAGAIRRLNRAGVLAVLVSNQAGVARGYFTIDVLEETFARLRELLARRNAHLDA
                     IYYAPFHPSSADPRWRDDPEELRKPGLGMIRRAQAELPIDMSRSYVVGDRQLDVQFAH
                     RAGLPAVFVKSGYGLGELTYSRATWREEPEHIAEDLPGAVAWILRDLKRRARPTAPSR
                     SNRS"
     gene            3919..4425
                     /locus_tag="PLS90_03430"
     CDS             3919..4425
                     /locus_tag="PLS90_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HPK01488.1"
                     /translation="MTPPLPASPRERIVDLAEAAEFARDRQRRGLRVVFANGCFDVLH
                     AGHVSYLNDARQEGDALIVGINSDRSVREIKGPDRPIVPQEDRAELIAAMEAVDRVVI
                     FDEPTAERCLRTIRPDVHAKGTDYTAETVPEREIARELGIRVAISGAPKTNASKQILR
                     AVREQQTP"
     gene            complement(4550..5833)
                     /locus_tag="PLS90_03435"
     CDS             complement(4550..5833)
                     /locus_tag="PLS90_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01489.1"
                     /translation="MLTTERPTVRVNDRLFDAGTLRILDLHADYQCANTGHCCGRAWN
                     IRISDREHGRLTDALRRAGRGPEEIERHFFAAGGDGAEQRWQFARAASGRCIFNGAGA
                     CGESTCNAQRELGHEVLPDVCQSFPRLAVATPAGIYLTLSYTCPTAVRSLLSEDRLAE
                     VAPRAVFQHHPRLQGASFGPGAELPWFGQQPTASWEAWDYFWRWASQFAARAEFTPAQ
                     ALYLIGQAVAHVERNAPATGRLDVLVDLLDQIGALPAADLRRHCAAIAPLTELGATYL
                     SVILNVLASCGVVPEILRGARETLARPGSPEREALLREYDARVRPRLAEFERIERNWV
                     ASRLYANPLIYQAKHLRVGYFLAVLNLIQWRFTVLLLALRDGTGIEEQILLRAAAETD
                     HLLQHNAAVTRRMLELLRREAAAAALEHLARPALF"
     gene            6118..7329
                     /locus_tag="PLS90_03440"
     CDS             6118..7329
                     /locus_tag="PLS90_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01490.1"
                     /translation="MTTVRKQVPRVLEGLLQISNFVGSVMLLDDILDHIARITSDIMQ
                     VPVCSIYLMNEEKELVLRSNVGFERELLGVARFALGEGIPGWIALENQTIALTDATTH
                     PRYKPIDSAIELGCRAYLAAPLRIQEEIIGVMTARKTEVYEFSRDEILFFETVCKQVA
                     IVIEKARMYESKLTVERLAAVAISLSGVAHYIKNVLLTMKGGEYLVDNGLAQGQLERA
                     REGWSVLKRANRKIRGLVENMLNFSRQSQLILRPVNLNSMVIDMLRSLEELAHERGVE
                     LVPALDGGIDEVSVDPESFYDVLLNLTTNAIEAMPEGRGGKVVVRTSRLDGRHQLLIE
                     VIDNGTGIPLEIQDRVFNLFFSTKGKRGTGIGLAATRKIVEEHGGTIEFSSTPGAGTH
                     FKVYMPTGRNE"
     gene            complement(7387..7515)
                     /locus_tag="PLS90_03445"
     CDS             complement(7387..7515)
                     /locus_tag="PLS90_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01491.1"
                     /translation="MFAVKLIVAAAVLYLIIAMALRHRRRTQGGGCQACCGHKREE"
     gene            complement(7515..9569)
                     /locus_tag="PLS90_03450"
     CDS             complement(7515..9569)
                     /locus_tag="PLS90_03450"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HPK01492.1"
                     /translation="MTLAPAPPDTPAHEATRTAPRRQTIALVGNPNAGKTTLFNALTG
                     LRAKTANFPGTTIELRVGALRLAGESVRLIDMPGLYSLSAASSEERAALDGLLGLAPE
                     HPAPTALILLLDSTNLERNLFLASQVLELGLPTVIALNMVDLAQRLGLRIKVAELSRE
                     LGCPVVPVVAKTGRGVDELRAELERVIARRPDVPHQPAAERARQLDTQSQHQRYDWAE
                     RVVGRCVVHPDRQIHGRSEAIDRVLTHPVVGVVAFLALMLGVFYLIFSLASIPMDLID
                     GVFGWLGDVAAGLIPAGPLQSLIVDGMIAGVGGVVIFLPQICILFFLIALLEDTGYLA
                     RAAFVMDRLMRRVGLPGKAFVPMLSAHACAVPAIMATKVIDDRRDRLVTILVLPLMTC
                     SARLPVYAMVTGLLFPTQPLLGAAAFTGAYSLGILAAISMALIFKRTILPGQARPLVL
                     ELPSYKMPSLKTAFFVMLDRGIIFLKKAGTVILALSIVLWWLANFPEMQPGDVPAGTA
                     AQVAALEAQAAALETAGQAEQAALAADQAADLISQQQLAHSFLGRLGHLIEPAVLPMG
                     FDWQLGVGVLASFAAREVVVSTLSILYGLGEEGAEGAALVDQMRAATRPDGSLVFTLP
                     VCFSLLVFYVLAMQCFATTAIAAREAGGWRWALLQLGYMTVLAYGAATLTYQGLTWLG
                     AA"
     gene            complement(9588..9869)
                     /locus_tag="PLS90_03455"
     CDS             complement(9588..9869)
                     /locus_tag="PLS90_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPK01493.1"
                     /translation="MSSATLESPGLVPLAGLGKRARGTIRLLEMAGDELNRLAGFGIC
                     MGRTVELIKRGDPLILRVYGTRIGLSARLAQCIHVEPADGASPDTAEPR"
     gene            complement(10123..10758)
                     /locus_tag="PLS90_03460"
     CDS             complement(10123..10758)
                     /locus_tag="PLS90_03460"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008951180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPK01494.1"
                     /translation="MTPVRPRCPWVPLDKLDYVAYHDAEWGVPVHDDIRIFEFLTLEA
                     AQAGLSWYTILRRREHYRTAFAGFDPARVARFTPARVERLLQNAGLIRNRAKINSAIN
                     NARRFLAVQEEFGSFDAYIWRFVGGKPIVHELRTLGDYPATSPESDALSRDLRQRGFT
                     FLGSTVVYAHMQATGLINDHTLDCFRRREILRLMRSDRPSRHSSSKTRPST"
     gene            complement(10975..12090)
                     /locus_tag="PLS90_03465"
     CDS             complement(10975..12090)
                     /locus_tag="PLS90_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPK01495.1"
                     /translation="MADFLRIAVIMAGGSGERFWPLSRRHRPKQLLRLATDQQTLLEE
                     AIERIEPLVPPERIFVITAAALVTPIREAHTGLPDENILGEPAKRNTAGALTWAAAHI
                     LANFAAQSAEKTSIAVLTADHRIGDRQGFAATVQSALTAAEQTGALVTIGVPPERPET
                     GFGYIEVPPDATPLLATDPPVLPVARFLEKPDRPTAEAFLRDGRHFWNSGMFFWRLDR
                     FLEELNGSAPAHAQAALSMALAMANRDPEAAARIFTELEDISIDYALMERAGRVAMAR
                     AAFDWDDVGAWDALDRNRARDAAGNVAVGDPVLVDSRDCIVYNAEGADAIAVGVVGME
                     GVVVVVSRDGVLVVPKDRAQDVKQVVGELKQRGAKQL"
     gene            complement(12369..13589)
                     /gene="dprA"
                     /locus_tag="PLS90_03470"
     CDS             complement(12369..13589)
                     /gene="dprA"
                     /locus_tag="PLS90_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPK01496.1"
                     /translation="MSQTAHDSAVDAAVLSWLRLALATGPVLGRRLLDAFGSPAAVFS
                     ASAAELQRVEGLGPTRLARLRDADVEAAARRECERARAVDVKLVPLDDPGYPPHLRRL
                     ESPPLVVWRRGGLIPADRMAIAVVGPRQPSVYARQMVDRIVRPLAAQGITIVSGMAYG
                     IDAEAHRAALEVGGRTLAVLGQGLATPIYPRAHAPLAAEILDRRRGALLSVLPCDTEP
                     SPGHFPLRNEIIAGVSLGVLVIEAGAASGTLITARHAAAANRPVMACPADATRRAAEG
                     SNRLIRDGAVLVRSADDVLETLANDLRHEMEELGVRAGQAAPAPEPEAGGAEGPPPAE
                     AAAAGPLPRPKDPLARLILAQLNEEPLPTDALLERCARQGHGMPAVLERLLQLEIDGW
                     LLQLPGRLYTLKGR"
     gene            complement(13621..14700)
                     /locus_tag="PLS90_03475"
     CDS             complement(13621..14700)
                     /locus_tag="PLS90_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01497.1"
                     /translation="MEDPVLNRNLEEIKELHTRWTQFRDFVLTAVKSRRATPQAEMKF
                     LELKSRIAMLQDSFMATLEHSHKTGQNIMTIVGDCILLQRVANYNDAERQKFEFDWNE
                     CYILLTEQIAHLEEEKQRLAGISEAAFKARRRKERIVAAVYNFIHSAGLKWTIVAAII
                     VLIVYVIPAYFYSYSAFHSMPVIKMVSLPIANYVYRPFLARDYEYRSMDWVERNKGYV
                     TNEIFPDARASGASDLTPEFFKMNVPRSLGIQPADVPQAIELFDRHIAMEVERFLTAG
                     IDMQFYYILFASSDDAKAFIDLARKGLDSLPQATRDAILNSTHVRRRANFVAIGIGSH
                     ALMLNGHIWRKFKFKEQQRSILEPQ"
     gene            complement(14851..15633)
                     /locus_tag="PLS90_03480"
     CDS             complement(14851..15633)
                     /locus_tag="PLS90_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK01498.1"
                     /translation="MQLENRVAYITGGGSGIGKAAAVLLAAEGARVCVTSRDLGDLEE
                     TVQEIEEGGGEAFAVAGDIARVEDVERAVRQVVERWGRLDIVFANAGINGVWAPIEEI
                     EPEEWDTTLNINLRGTFLTLRAAMPHLKRQGGSIIITSSINGTRVFSSQGATAYGCSK
                     AAQVALCKFVALESARHGVRVNVICPGAIATDIDSSTEMRDADEAAPPLAFPENPIPL
                     SGGKPGDPHQVARLVLFLASDASSHITGTEIWIDGAQSLLRG"
     gene            complement(15806..16159)
                     /locus_tag="PLS90_03485"
     CDS             complement(15806..16159)
                     /locus_tag="PLS90_03485"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HPK01499.1"
                     /translation="MAPSEDREVCLCFHVPLGKLRRFHERRRARVASQFAECHGAGTG
                     CGWCVPYLQQIFEQLERGEEPRLAMSAEEYRARRIAYHKEKKPELPPPADADGPIALD
                     LDELLGDVPDDLKLD"
     gene            16544..17344
                     /locus_tag="PLS90_03490"
     CDS             16544..17344
                     /locus_tag="PLS90_03490"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPK01500.1"
                     /translation="MDLTLNRRDILTRTLPAAAFVGASLGSLGTLMAQPPAQGQMGQK
                     SIIGSMIMQAAGDGQYKLPPLPYDYDALDPHIDAQTMQLHHDKHHQGYVDGLNKAVQG
                     MTEMASGEIDDAKLAALERNLSFNGGGHLLHTIFWATMSPDGGGAPEGELAEAINAGF
                     GSVDGFRNYFSKAAGGVKGSGWGLLAYEPLGDRLIVFALNEHDTKLMAGACPLLPLDV
                     WEHAYYLKYQNRRADYIKAWWNVVNWKAVGEAYAWARKAFHGRKGEGE"
     gene            17607..17951
                     /locus_tag="PLS90_03495"
     CDS             17607..17951
                     /locus_tag="PLS90_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HPK01501.1"
                     /translation="MGIVTDATDKHQACIDACQTCSQACYECFRACLKEPDVAQRTEC
                     IALLVECAMMCQTSVAHMAMSGRFSADHCRHCAAVCEACAEECGHHRDEHCPKCAEVC
                     RRCAAECRRMAA"
     gene            complement(18279..20210)
                     /locus_tag="PLS90_03500"
     CDS             complement(18279..20210)
                     /locus_tag="PLS90_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01502.1"
                     /translation="MGTPLRVLIAEDSPDDVLLLLREIRRGGYDPDYIAVDSAAQFDQ
                     ALADGEWQVILADYAMPAFTGLDALRRVRNRGLDTPFILISGTIGEELAVEALKQGAN
                     DFLMKGRLGRLLPMIRRALNEVEQRREIARVENSLRETYHEMEGLIAASPMAIVAVDR
                     EGRVTVWNESAEKLFGWTREEVLGQPPPFIGSGDELALHFLLCPTGPDQIPTFLEMQI
                     CTRADVNRRVDLELYSAPLIDSAGGFRGVISYISDVTERKILEKSLRQSQKMEAIGQL
                     AGGVAHDFNNLLQAIIGYTQVAMETLPPDAPVRADLQQSINAGQKAATLTRQLLAFSR
                     RQILQPKDVDLNQLVGGFLRMLERVIGEHVTLQFRPAENLGTVSVDPGQIEQVIMNLV
                     LNARDAMPDGGSILIQTSNADISSAYKTSHPWASEGPYVMLTVTDTGCGMDEATQSRI
                     FEPFFSTKPEGEGTGLGLATVYGIIKQHGGFISLYSEPGHGTSFKIFLPLVNRSAAEL
                     GLREMGDVVGGNETILLAEDSETVRDLSVRILRGAGYRVLTACDGEEAVATFAAHADQ
                     IDLVLLDVIMPRLHGRAAFERISAIRPGVRVAFSTGYSTHGLHDHLIPDGDPHLIQKP
                     YNPKDLLRFVRETLDAAPR"
     gene            complement(20216..20662)
                     /locus_tag="PLS90_03505"
     CDS             complement(20216..20662)
                     /locus_tag="PLS90_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01503.1"
                     /translation="MPDDKTILLVEDNPDDVALTLRAFRKQNIHNEIVVAEDGVEALD
                     YLHGRGRYSGRDASQQPTIILLDLQLPRMNGLEFLKELRSHPETKLMPVIVLTSSNEE
                     RDLVESYSRGANSYVRKPVEFSEFLNAVQTLGLYWLLINTRPPQAK"
     gene            complement(20652..22040)
                     /locus_tag="PLS90_03510"
     CDS             complement(20652..22040)
                     /locus_tag="PLS90_03510"
                     /inference="COORDINATES: protein motif:HMM:NF017077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HPK01504.1"
                     /translation="MPRSGMRRHRLWMTLAIAVLVVDVIAGILVTRRQTLFHAEISQS
                     YLVLLQLQAILSSLQDMETGQRGYLLTGDHDFLEPYLRGRRQLDGQIDQALRTSRNNP
                     EHYQNLLKLRAAASTRTITLEENLNIFEAEGLDPARAHVRLGYGKREMDSVRALIGGL
                     IRDERNRLDSRLHRASHYSQFMLISLLGGAVAVVLLLIWCFVLLTRELKGRAERTRDL
                     ERRVEERTRRLTEVNQELEAFCYSVSHDLRAPLRSISGFSEALLEDLGPRLEEQECDY
                     LRRVVAASKRMGVLIDDLLNLSRVSREEVRKDELDLSAEAQAVAEECRRGGEYDGVAV
                     EIQPQLTAKGDRRLIRIALVNLIDNAFKFTRPVEAPRIQVSQVAEQNGERIFCVTDNG
                     VGFDMEYVHKLFSPFQRLHRPQDFEGTGIGLAIVRRVVQRHGGRVWAESIPGQGTRMY
                     FTLPKEDSIHAG"
     gene            complement(22615..23097)
                     /locus_tag="PLS90_03515"
     CDS             complement(22615..23097)
                     /locus_tag="PLS90_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK01505.1"
                     /translation="MPRIRQKITPFLWFDNQAEEAARFYTSIFADSRIIDVARYTAAN
                     PDREGTVMLVTFELAGQRFYAMNGGPNQPFNESISLLVECETQEEIDTLWGRLTDGGT
                     EIQCGWLKDRYGLAWQITPSRLLELVSGPDRDRAAAAMRAMFEMKKIVIADIERAVST
                     "
     gene            complement(23363..23989)
                     /locus_tag="PLS90_03520"
     CDS             complement(23363..23989)
                     /locus_tag="PLS90_03520"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HPK01506.1"
                     /translation="MRSLRVRVAISLVLMGLGAGGSALMLFPTSQVRGSDTAEHLPME
                     TGDWYGQRVPVEEYVKQILETDDVIQRNYINPLLSPVPVQLAVVFSPDNRRVAHPPEV
                     CYRAAGWEAMDKRVIEPAGLPPMMRVKMSYGGRDDLILYCYKAGDELTPNYYNQQLKT
                     VYNQLRMQPTVSALIRFSTAIGEDEAEAEARILALASEMLPHIMTTLQ"
     gene            complement(23982..24950)
                     /locus_tag="PLS90_03525"
     CDS             complement(23982..24950)
                     /locus_tag="PLS90_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HPK01507.1"
                     /translation="MTFTSPRQDPLQRISPAGWTAVAALAALFVLAFRVPIAWMVDRW
                     VAPESYYSHGFIVPLVALYVVWRDRFELAARPFATPGAGLFVMLAGLLMLFVFGWLTI
                     FSPAAFAMIFVVWGLCGFLFGWPVFKRLAFAAFILTFMVPLPLAIIEKISFAMKMLAG
                     TLALKMIGLLGITAINDGATILLGDAAVTVGNACSGLRSLISLIFLGLLFAYLSDLTP
                     ARRAILFAASIPIALISNVLRVFFLCMVAYRWGDRAIEGTVHDVSGYMIFVIAFVLLY
                     ATARALGWNRGDGGGGSAGEVEDAPPRPAGEPAQPIPARPRESTHA"
     gene            complement(25139..26113)
                     /locus_tag="PLS90_03530"
     CDS             complement(25139..26113)
                     /locus_tag="PLS90_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPK01508.1"
                     /translation="MMTAGPEKTIFDEAVADQCEETIQNRAQRPVLIVLDENEANRRI
                     PVLKRELILGRDLMCDIALHDSRCSRRHARMVYENFNDPVASPLVRVHDLDSTNGTFV
                     NGTRVSDHLLQDRDKILIGSSVIGFFLRDESELAVDQRLFQLASQDPLTGLSNRGVFD
                     VEIKQEFDRAIRYGRRLALVMFDIDHFKRFNDTYGHPVGDFVLRELAALIQTKVRGND
                     ICTRYGGEEFAIILPETSREGALIQAERLREAVEHHSFERDGHTLTLTISVGIASIDG
                     GYRSAEEMINAADRALYSAKAGGRNKVCIASEAHVVDLGDKTGGGFAI"
     gene            26395..27705
                     /gene="hcp"
                     /locus_tag="PLS90_03535"
                     /gene_synonym="priS"
     CDS             26395..27705
                     /gene="hcp"
                     /locus_tag="PLS90_03535"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPK01509.1"
                     /translation="MYCDQCEQTFRGSACTEVGVCGKDPDVESLQKILLYGLKGMAAY
                     KHHARRLGKTDPEVESFIEEALFATVTNVNFDPPSLLKLVLECGRMNLRTMQLLNDGH
                     VETFGTPSPVEVSEGIQEGPGILVTGHDLVDLRDLLEQAAAAGVHVYTHGEMLPAHMY
                     PGLRKYPHLKGHYGDAWQKQRDEFERFGGPIVATTNCVQIPRQENTYLDRFFSTRTTA
                     IPGGRLIEGNDFGPVIRRALEIGPLRPTPIRRSTIGFHYTTLLAVADKVVAAVKAGQV
                     RHFYLIGGCDGAEPGRNYFAHYAQNTPPDSLILTLGCGKFRIRNHDYGTIRLGDLEVP
                     RLLDMGQCNDAYGAIQVALALAEAFQCGVNDLPLTLVISWFEQKAVAVLLTLLHLGVR
                     GIRLGPALPAFVTPNVLAMLQEHYDLQPIGQDAAADVARTLAAV"
BASE COUNT         4873 a         9073 c         9595 g         4636 t
ORIGIN      
        1 tttcttctct cgccctcgac tcttgaatca acttgcattt catcgataac cgcgagggca
       61 tggctaactc gtcacgaagc cccgctctac acgtccccat cctgcacgcg gatccggggc
      121 accaggtcgg ccggttcgcc ggcatactca tccgaatcct gcttgtaata gaaactggtg
      181 gcgccgccga gcgggaaatc cttgcgcagc ggatggccct cccagttgtc cggcatcagg
      241 atccgctgca gattcggatg gccgtcgaag cggatgccga acatgtcgta gacctcgcgc
      301 tccatgaagt cggctccctt ccacaggtcc gtgatcgaat cgatccggca ctcctcctcg
      361 tcctcgccga cgggaacccg caggcgcagc cgatgggcgt gctccagcga gccaaggtgg
      421 taaaccacat caaagcgcgg ttcgcgaccc agttccaggt aatcgacggc ggtcacatcg
      481 ctcagcatcg tcagcgacag cccggcgtcg tcgcgcagcg ccttgacggc gtcgtggatg
      541 tagcgcttat cgatcagcag cgtcacctcg ccgctgggcg cgacgatccg ctccgccacg
      601 cagtccggcg cgatctggtt caaaacctga accgccgcct ccgccaggtt ctcgtttgca
      661 agtgtcgcca tcttcctctc ccgcgcgtct cagccttcgc gaatcgccgc caatctctcg
      721 ggtgagtcag gtcttgcaag gtcgccgcgt cgtcatccgc attcagtggc cttgccgatc
      781 tccttcaagg agccttcttc ctcagccctc cccccggggg gtgataatcc gctgcgggct
      841 gtgctgcacc tttttctgca actgcaggat gccgtagatc agcgcctcgg gccgcggcgg
      901 gcagcccggc acgtagacat cgaccgggat aatctgatcc accccctgca gcaccgagta
      961 aacattgtgc atcccgccgc acgaggcgca cgcccccatc gcgatcaccc atttgggctc
     1021 ggtcatctgg tcgtagatcc gccgcaccac cggcgccatc ttcttgttca cccagccggc
     1081 gacgatcatc agatcggcct ggcgcggcga ggggcgaaac acctccatgc cgaaccgcga
     1141 caggtcaaac cggctggcgg cggtccccat catctcgatc gcgcaacagg ccagccccag
     1201 catcaccggc cagatcgagt tcttgcggca ccatgccacc acctcgtcca gcgttttgac
     1261 cagttgattg tcgtattcca gcgggcgcag cttgtcctgc agcgcgagcg cttcgcggtt
     1321 gatcagcggc ggccgcttca gcggatcggc cagcaccagc ccccccgcgt cctcgggggg
     1381 tcgtcggtcc aggtccctta tgtccattcc agtgcccctt tccgccattc gtagatgaag
     1441 ccaaccacga ggatcagcag gaagaagacc atcgccagaa aggcataggg ctggaaattg
     1501 gggatcgtgg ggtcccgaaa cgcaatcgcc caggccagga tcagcaccgt ttccatgtcg
     1561 aacagcagga agagcaacgc gaccaggtag aatttgatcg gaatgcgaac attggcctcc
     1621 cccgtcggca cgttgccgca ttcatagggc agcagtttgg tgaaggtgat gacgcggggg
     1681 ccgatcagcc ccgaaagcgc cagcgtcacg atcccgaagc ccagcgcgat caccagcagc
     1741 atcaggatcg ggatgtaggg tgccagatag tccatcggtg gtggccccgc ggagaggttg
     1801 aggtccccgg cgagttcggg gggacggaaa cgagcgtcgg cgggatgagc gggtgggaga
     1861 cgtgcgcggc cgtaacccgc aataagctat acatcggtca ttttttcaca atctcctgca
     1921 ccagtcaaac cgattcgccg acgccgcccg gtggctctcc ggcccggccc ggccggcctg
     1981 cgggcccggc tgttgcgaat cgggtctcga ccgcggcggc tggggcacga tctcccgtcg
     2041 gccaatgggc gcgaggccat cgggaactgc cgacaagccg ccgccctatt cagccatacg
     2101 ccccactcgg acgcaaccca aacatcccgg tatcttgccc ggtcaaccaa acctccgccg
     2161 gcggcgctcc ggcctgattt tgcgggtcat gcgcgctgtg ggaatcggct ggaattgcgc
     2221 tttttttcac aagatcctcc ggcgccgacc cgcatcggcc cttttgtttg actatttcga
     2281 tccccgcatc aagaatatgg cggcttcccc tccggcgcgg gccagaccgc gcgcgcgacc
     2341 gcgcagaaag ggctccccca tgcaatcgtc cacgcctcct gtccgtcccg tgccccaccc
     2401 ttggtcggcg tttaccctca tcgaactgct catcgtcgtg gcgatcatcg ccatcctggc
     2461 cgcgatcgcc gtccccaatt ttctggaggc gcaggttcgc gccaagagct cgcgcgccca
     2521 ggccgacatc cgctcggtcg ccaccgcgct ggagatgtat cgcgtcgact acaacgccta
     2581 tcccaccatg atcgagcccg gatttcccca cgtctacggg ctgaccgacc tgaaatggtg
     2641 gtatgtgccc gacgcgctga gcacccccgt cgcctacctc ggcagcgccg atctgcactg
     2701 tccgttcggc ggcgatttca agaatcagcg cgacaatttc cccggcgaga tctggcgccg
     2761 ctactcctac gaaaacatcc gtgaactgga acagtatgcc gccagctatc ccctgctgca
     2821 ggccaaatac ggcccccagc agcatgccct ggagcggatc ggcggctggc gcctgctctg
     2881 catcggtccc gacatcgaat ggaacccgat ggttcagtac gatccgacga acggcacggt
     2941 cagcctgggc aacatcatgc gctcgcagaa agaccccgtc ggccgcaaca gcgaaccgct
     3001 ggccgtcaac tgaagcccca tcccgcgccg ctcgccctcg tcctcaaccg gtgtaggtct
     3061 gcctaagatt cggctcccgc ctctctttct tcatccgcgg cgctccagtt cctgttttcg
     3121 aatccataaa tcggttcgtt aatttggttg gagatcgttc ctgcgccctt ttgcttttca
     3181 aatggcgcgg ggaatcgcag aatggctcgg cgtgccgccg cgcgccgctg ctctccgtcg
     3241 atggaacgac gccatggcgc ataacccgtc aactcccgcc gaccgcccct ggcccgccga
     3301 tcaactgcgg ccgtgcgtct ttctggaccg ggatgggacc gtcacccgcg aggcgggcta
     3361 catcaaccac cccctccggt tggaactgct cgccggagcg gccggcgcca tccggcggct
     3421 caaccgggcc ggcgtgctcg ccgtcctggt cagcaaccag gcgggcgtcg cgcgcggcta
     3481 cttcacgatc gacgtgctgg aggagacctt cgcgcggctg cgcgaactgc tcgcccgccg
     3541 caacgcccac ctcgacgcga tctactacgc gccgttccat ccgagcagcg ccgacccgcg
     3601 ctggcgcgac gatccggagg aactgcgcaa gcccggcctc gggatgatcc gccgcgcgca
     3661 ggccgagctg ccgatcgaca tgtcgcgttc ctatgtggtg ggcgatcgcc agctcgacgt
     3721 ccagttcgcg caccgtgccg gcctgcccgc cgtctttgtc aagtcgggct atggtttggg
     3781 cgaactcacc tactcgcgcg cgacctggcg cgaggaaccc gagcacatcg ccgaggatct
     3841 gcccggcgcg gtcgcgtgga tcctgcgcga tctgaaacgc cgcgcccgcc caaccgcccc
     3901 atcccggagt aaccgatcat gaccccgccc ctgcccgcca gcccgcggga gcgcatcgtc
     3961 gaccttgcgg aagcggccga attcgcccgc gaccgccagc ggcgcggcct gcgcgtcgtc
     4021 ttcgccaacg gctgcttcga tgtgctgcac gccggacacg tttcgtatct gaacgacgcc
     4081 cgccaggagg gcgatgcgct gatcgtcggg atcaattcgg accggtcggt gcgcgagatt
     4141 aaggggcccg accgtccgat cgtgccgcag gaggaccgcg ccgagctgat cgccgcgatg
     4201 gaagccgtcg atcgggtcgt gatcttcgat gaaccgaccg ccgagcgctg cctgcggacg
     4261 atccggcccg acgtccatgc caaggggacg gactatacgg ccgaaaccgt gcccgagcgc
     4321 gagattgcca gggagcttgg gattcgcgtg gcgatcagcg gcgcgcccaa gaccaacgcc
     4381 agcaagcaga tcctccgcgc cgtccgcgag caacagactc cctgaacctg tgcagagcat
     4441 ccggcgttat taaagtaaag cttacgttga tgccgacggt gtgggcatga agaatcgcct
     4501 cgcggccagc ctgtcatctc tccgagggtg ctgaaccccg ccgatcggcc tagaacaacg
     4561 ccggacgggc aaggtgttca agcgcggcgg cggcggcctc gcgccgcaac agctcgagca
     4621 tccgccgcgt cacggcggcg ttgtgttgca gcaggtgatc ggtctcggcg gcggcccgca
     4681 gcagaatctg ttcttcaatg ccggtcccgt cgcgcagcgc cagcagcagc acggtgaacc
     4741 gccactggat caggttcagc accgccagaa aatagccgac gcgcaggtgt ttcgcctgat
     4801 agataagcgg gttggcgtaa agccgcgagg cgacccagtt gcgctcgatc cgttcgaatt
     4861 cagcaagccg gggacgcacc cgggcgtcat actcgcgcag cagggcctcg cgttccggcg
     4921 atccggggcg cgccagcgtc tcgcgcgcgc cgcgcaggat ctcgggcacg acgccgcagc
     4981 tcgccagcac gttgaggatc acgctcaggt aggtcgcgcc cagctcggtc agcggcgcga
     5041 tcgccgcgca gtggcgccgc aggtcggcgg ccggcagcgc gccgatctgg tcgagcagat
     5101 cgacgagcac gtcgagccgg ccggtggccg gggcattccg ctcgacatgg gccacggcct
     5161 ggccgatcag atacagcgcc tgggccgggg tgaactccgc gcgggcggcg aactgcgacg
     5221 cccagcgcca gaaataatcc caggcctccc agctggcggt cggctgctgg ccgaaccagg
     5281 ggagttcggc gcccgggccg aagctcgccc cctgcagccg ggggtgatgc tgaaacaccg
     5341 cccgcggcgc gacttccgcc agccggtctt cgctcaggag cgacctgacc gccgtcgggc
     5401 aggtgtagct cagcgtcagg tagatgccgg cgggtgtggc gacggccagc cgcggaaaac
     5461 tctggcagac gtcgggcagg acctcatggc ccagttcgcg ctgggcgttg caggtcgatt
     5521 cgccgcaggc gcccgcgccg ttgaagatgc agcgccccga cgcggcgcgc gcgaactgcc
     5581 agcgttgctc cgcgccgtcg ccccccgccg cgaaaaagtg ccgctcaatc tcctccgggc
     5641 cgcggccggc gcgccgcagg gcatcggtca gccgcccgtg ttcccggtcg ctgatgcgga
     5701 tgttccatgc gcgcccgcag caatggccgg tgttggcgca ctggtagtcg gcgtgcaggt
     5761 cgaggatgcg aagcgtgccg gcgtcgaaca ggcggtcgtt gacgcgcacg gtgggccgtt
     5821 cggtggtcag catggcgtct tccggggcgc cgtcggcgcg ggatgggagg gcgcggggcc
     5881 aggccgccgg ccgcccgcgc atacgttcct tatcgggcgc tcgaccggcc ggctgaaccc
     5941 gccggttgcc ggcgaaggca cgcgccggcg cggcttcctg aaatcgccgc cgacgaaacg
     6001 ggatggttct ttcaccggcg gggatggtaa gctggcgcgt gtggtgggtg ttggccgccg
     6061 gcgatggtgc ttcgccccgg cacgcccttc ccgccgacca tcccttggag ttgcgtggtg
     6121 acaacggttc gcaaacaggt tccgcgggtt ctggagggtt tgctccagat cagcaatttc
     6181 gtcggttcgg tgatgctgct cgacgacatc ctggatcata tcgcgcggat caccagcgac
     6241 atcatgcagg tgccggtctg ctcgatctac ctgatgaacg aggagaagga actggtcctg
     6301 cgcagcaacg tcggcttcga gcgcgaactg ctcggcgtcg cgcgcttcgc cctgggcgag
     6361 gggatcccgg gctggatcgc gctggagaac cagacgatcg cgctgaccga cgccacgacc
     6421 cacccgcgct acaaaccgat cgattcggcg attgagttgg gctgccgcgc ctacctcgcc
     6481 gcgccgctgc gcatccagga ggagatcatc ggcgtgatga ccgcgcgcaa gaccgaggtc
     6541 tacgaattca gccgcgacga aatcctgttc ttcgagacgg tctgcaagca ggtggcgatc
     6601 gtgatcgaga aggcccggat gtatgagtcg aagctgacgg tcgagcggct ggccgccgtc
     6661 gcgatctcac tctcgggcgt ggcgcactac atcaagaacg tgctgctgac gatgaagggg
     6721 ggcgagtatc tggtcgacaa cggcctggcg cagggccagc tggagcgcgc ccgcgagggc
     6781 tggtcggtgc tcaagcgcgc caaccgcaag atccgcgggc tggtcgagaa catgctcaac
     6841 ttcagccggc agtcgcaact gatcctgcgg ccggtcaacc tcaacagcat ggtgatcgac
     6901 atgctgcgct cgctcgagga actggcgcac gagcgcgggg tcgagctcgt gccggccctc
     6961 gacggcggca tcgacgaagt gtcggtcgac cccgaatcgt tctacgacgt cctgctcaac
     7021 ctgacgacca acgcgatcga ggcgatgccc gaggggcgcg gcggcaaggt cgtcgtccgg
     7081 acgagccgcc tcgacggccg ccatcagttg ctgatcgagg tgatcgacaa cggcaccggc
     7141 atcccgctgg agatccagga ccgggtcttc aacctgttct tctcgacgaa gggcaagagg
     7201 ggcacgggca ttggcctggc ggcgacccgc aagatcgtcg aggagcacgg cggcactatc
     7261 gaattctcct ccacccccgg cgccggaacg catttcaagg tctacatgcc gacgggaaga
     7321 aatgaatgag gggataaatt cgagatacga acgtcgattt tcgagattcg gatttcgggc
     7381 ttccgcttat tcttcgcgct tgtgcccaca gcaggcttgg cagccgccgc cctgggtccg
     7441 gcggcggtgg cgcagcgcca tggcgatgat caggtagagg acggcggcgg cgacgatcag
     7501 tttgacggcg aacatcaggc ggctcccagc caggtcaggc cctgatacgt gagggtggcg
     7561 gcgccgtagg ccagcaccgt catgtaaccg agttgcagca gcgcccagcg ccagcccccg
     7621 gcctcgcgcg cggcaatggc cgtcgtggcg aaacactgca tcgccagcac atagaacacg
     7681 agcaggctga agcagaccgg cagggtgaag acgagcgagc cgtcgggccg ggttgcggcg
     7741 cgcatctgat cgacgagcgc ggccccctcg gccccttcct cgcccaagcc gtaaaggatc
     7801 gacagcgtgg agacgacgac ctcgcgggcg gcgaacgagg ccagcacgcc cacgccgagc
     7861 tgccagtcga accccatcgg cagcacggcg ggctcgatca ggtggcccag ccggccgagg
     7921 aaggaatggg cgagttgctg ctgggaaatc aggtcggccg cctgatcggc ggcgagggcg
     7981 gcctgttcgg cctgaccggc agtttccagc gcggcggcct gggcctcaag cgccgcgact
     8041 tgcgccgccg tccccgccgg gacgtcgccc ggctgcattt cggggaaatt cgccagccac
     8101 cacaggacga tcgacagcgc cagaatcacc gtgccggcct tcttcaggaa aatgatcccg
     8161 cggtcgagca tgacgaagaa cgcggtcttc aggctgggca tcttgtaact gggcagctcc
     8221 agcacgagcg ggcgcgcctg gcccggcagg atcgtgcgct tgaaaatcaa cgccatcgag
     8281 atcgcggcca ggatgcccag actgtaggcg ccggtgaacg ccgcggcccc cagcagcggc
     8341 tgcgtcggaa acaacagccc cgtcaccatc gcatagaccg gcaggcgcgc cgagcaggtc
     8401 atcagcggca gcaccaggat cgtgacgagc cggtcgcggc ggtcgtcgat gacctttgtg
     8461 gccatgatgg ccggcacggc gcaggcgtgg gccgacagca tcggcacaaa cgccttgccg
     8521 ggcaacccga cccggcgcat caggcgatcc atgacgaacg cggcgcgcgc caggtagccg
     8581 gtgtcctcca gcagcgcgat caggaagaac aggatgcaga tctgcggcag gaagatcacg
     8641 acgccgccga cgccggcaat catgccgtcg acgatcaggc tctgcagcgg gccggcgggg
     8701 atcaggccgg ccgcgacatc gcccagccag ccgaagacgc cgtcgatcag atccatcggg
     8761 atcgaggcga gactgaaaat cagatagaac acgcccagca tcagcgccag gaacgccacg
     8821 acgccgacga cggggtgcgt caggacgcgg tcgatcgcct cgctgcggcc gtgaatctgg
     8881 cggtctggat gaacgacgca gcggccgacg acgcgctcgg cccagtcata gcgctggtgc
     8941 tgcgactgcg tatcgagctg gcgcgcccgc tcggcggccg gctggtgcgg cacatcgggg
     9001 cgccgcgcga tgacccgctc cagttcggcg cgcagctcat cgacgccgcg gccggtcttg
     9061 gcgacgaccg gaaccaccgg gcagcccagc tcgcgcgaca gctcggcgac cttgatccgc
     9121 aacccgagcc gctgggccag atcgaccatg ttgagcgcaa tgaccgtcgg cagccccagc
     9181 tcgaggacct ggctggcgag gaacaggttg cgctccaggt tggtcgagtc gagcagcagg
     9241 atcagcgccg tgggggcggg gtgctcgggg gcaaggccga gcaggccgtc gagcgcggcg
     9301 cgttcctcgc tgctggcggc cgacagactg tagaggccgg gcatgtcgat cagccggact
     9361 gactcgccgg ccagacgcag cgcgccgacg cgcagctcga tcgtcgtgcc ggggaagttg
     9421 gcggtcttgg cgcgcaggcc ggtcagggca ttgaacagcg tggttttgcc ggcgttgggg
     9481 ttgcccacca gcgcgatcgt ctggcgacgg ggggccgtgc gggtggcctc gtgcgcaggg
     9541 gtgtcgggcg gggcgggagc cagcgtcatg gtgggaaccg atcgaggtca gcgaggctcg
     9601 gcggtgtccg gcgaagcacc gtccgccggt tcgacatgga tgcattgcgc caggcgcgcc
     9661 gacaggccga tgcgcgtgcc gtagacgcgc aggatcagcg gatcgccgcg cttgatcagc
     9721 tcgacggtgc ggcccatgca gatgccgaag ccggcgaggc gattcagttc atcgcccgcc
     9781 atctccagca ggcggatggt gccgcgggcg cgtttgccga gaccggcgag cggaacgagg
     9841 ccgggggatt cgagggtggc actgctcatg agaaaagggt tgcaatctgg aagggggatc
     9901 gggacgatgg gagcccatcg gttgcccata gtaaacatta cttacccgcg acaatcaaga
     9961 gacattttca ttcccaaatc cgcttttttg ggtcccaagg tctcttatcg gctcgcccgc
    10021 cctgcccgat agccccggtt cgaggagaac gcggccggag cgcgcgcgca tgcccccgcg
    10081 gttgccaacc cgggaagaaa aaccggaagt tcggggcgtc catcacgtcg acgggcgcgt
    10141 tttcgaactg gaatggcggc tcggcctgtc ggaacgcatg aggcggagga tctcgcggcg
    10201 gcggaaacag tccagcgtat gatcgttgat caggccggtg gcctgcatgt gggcgtagac
    10261 gacggttgaa ccgagaaagg tgaagccccg ctggcggaga tcgcggctca gcgcatcgga
    10321 ttcgggcgag gtggccgggt agtcgcccag cgtgcgcaat tcatggacga tcggcttgcc
    10381 gccgacgaaa cgccagatgt aagcgtcgaa cgaaccgaat tcctcctgga ccgcgaggaa
    10441 ccggcgcgca ttattaatcg ccgagttgat cttggcgcga ttgcggatca ggcccgcgtt
    10501 ctggagcagg cgctcgaccc gcgccggtgt gaaccgggcg acgcgggcgg gatcgaagcc
    10561 ggcgaaggcc gtgcggtaat gctcgcgccg gcgcagaatc gtgtaccagg agagcccggc
    10621 ctgcgcggcc tccagggtca ggaattcaaa aatccggatg tcgtcatgga ccggcacgcc
    10681 ccattccgcg tcgtgatagg cgacgtagtc gagtttatcg agcgggaccc aggggcatcg
    10741 ggggcggacg ggcgtcatgg tctcggcctc acgcttaaat cgtccttcgt cctcgtcctc
    10801 gtgatcatga tcgattcgag gggttctgcg gtccctgcgg gactcttttg gctcctggtt
    10861 cccgttttcc cgccagtgaa caggcgggct gaaaatctgc catccctacg ggcctgacct
    10921 ggggagcagc ccggcatggc ctcagccatt caaattgatc gctggcggca gccgctacag
    10981 ttgcttcgcg ccgcgttgct tgagttcccc caccacctgc ttgacgtcct gcgcgcggtc
    11041 cttgggaacg acgagcacgc cgtcgcggct gacaacgacg acgacccctt ccatgccgac
    11101 tacccccacg gcgatcgcgt cggcgccttc ggcgttgtag acgatgcagt cgcgcgaatc
    11161 gacgaggacg ggatcgccga cggcgacgtt gcccgccgcg tcgcgcgcgc ggttgcggtc
    11221 gagcgcgtcc cacgcgccga catcgtccca gtcgaacgcc gcacgggcca tcgccacgcg
    11281 gccggcccgc tccatcagag cgtaatcaat cgaaatgtcc tccagctccg tgaagatccg
    11341 ggccgccgct tccgggtcgc ggttcgccat ggccagcgcc atgctgaggg cggcctgggc
    11401 gtgcgcgggc gccgacccgt tgagttcctc caggaaccgg tcgagccgcc agaagaacat
    11461 gccgctgttc cagaagtggc ggccgtcgcg gaggaaggct tcggccgtcg ggcggtcggg
    11521 cttctccaga aaccgcgcca ccggcagcac gggggggtcg gtcgcgagca gcggtgttgc
    11581 atcgggtgga acctcgatgt agccgaaccc ggtctcgggc cgctcggggg ggacgccgat
    11641 cgtgacgagc gcgccggtct gttcggccgc ggtcagggcc gactggacgg tggcggcgaa
    11701 gccctggcga tcgccgatgc gatggtcggc cgtcagcacg gcgatcgagg ttttctcggc
    11761 cgattgcgcc gcgaaattcg ccaggatgtg cgcggcggcc caggtcagcg cgccggcggt
    11821 gttgcgcttg gccggctcgc cgaggatgtt ttcgtcgggc aggccggtgt gcgcctcgcg
    11881 gatcggcgtc accagcgccg cggcggtgat cacgaaaatg cgttcgggcg gcaccagcgg
    11941 ctcaatccgc tcgatcgcct cttcgagcag cgtctgctgg tcggttgcga ggcgcagcag
    12001 ttgcttgggc cggtgccggc gcgagagcgg ccagaaccgc tcgcccgatc cgcccgccat
    12061 gatgacggca atgcgcagaa aatccgccat gcgtcgcttc cttccgatca gatcctcatc
    12121 cccgaatctg catggctacc agaggcggga ccgcaagcgc aagcggaaag ggttcccccg
    12181 ctgcgcttgg gcgaagcttg acacggttca agaggtgatc tgtttttcgg ccggtaaacg
    12241 gcgcgctggg gttgccggtc tagccgaacg cgccggtgat gatgtggagg cgcgacatgg
    12301 gtgaaccact gcatgaaatt cggtaacgcg tcgggtttgg gatcgtttaa ggctagaatc
    12361 agcgttcgct accggccctt gagcgtgtac aaccggcccg gcagttggag cagccagccg
    12421 tcgatttcca actggagcag ccgctccagg acggcgggca tgccgtgacc ctggcgggcg
    12481 cagcgctcca gcagcgcgtc ggtcgggagc ggttcctcgt tgagctgggc caggatcagc
    12541 cgcgccaggg gatctttcgg ccggggcaac gggccggcgg cggcggcctc cgccggcggt
    12601 gggccttccg ccccgccggc ctcgggctcc ggcgcgggag cggcctgtcc ggcgcgcacg
    12661 cccaactcct ccatttcatg gcgcaggtcg ttcgccagcg tctccagcac atcgtcggcc
    12721 gaccggacca gcacggcccc gtcgcggatc aggcggttgg aaccctccgc ggcgcggcgc
    12781 gtggcgtcgg ccgggcaggc catcaccggg cggttggccg ccgccgcgtg gcgtgcggtg
    12841 atcagcgtcc cgctggcggc gcccgcctcg atcaccagca cgccgagcga gacgccggcg
    12901 atgatctcgt tgcgcagcgg gaagtggccg gggctgggtt ccgtgtcgca gggcaggacg
    12961 ctgagcagcg cgccgcggcg ccggtccaga atctcagcgg ccaagggtgc atgggcccgc
    13021 ggataaatcg gcgtggcgag gccctggccg agcacggcca gggtgcgccc gccgacctcc
    13081 agcgcggcgc ggtgcgcctc ggcgtcgatg ccgtaggcca tcccgctgac gatcgtgatc
    13141 ccctgcgcgg cgaggggacg cacgatccgg tcgaccatct ggcgcgcata gacgctgggt
    13201 tgacgcggcc cgacgacggc aattgccatc cggtcggcgg ggatgagccc gccccggcgc
    13261 cagacgacca gcggcgggct ttccaggcgg cgcagatgcg gcgggtagcc cgggtcgtcc
    13321 agcgggacca gcttgacatc caccgcccgc gcccgttcgc actcgcggcg ggccgccgcc
    13381 tccacgtcgg cgtcgcgcag ccgggccaga cgcgtcggac ccagtccctc cacgcgctgg
    13441 agttccgcgg cggaggctga aaagacggcg gcgggggaac cgaacgcatc cagcagccgg
    13501 cggccgagca ccggcccggt ggcgagcgcc agccgcagcc aggagagcac ggccgcatcg
    13561 accgcggaat cgtgggcggt ctggctcacg tcgtgccccc ctgaattcag ggccgcgcga
    13621 tcattgcggc tccagaatgc tgcgttgctg ttccttgaac ttgaacttgc gccagatgtg
    13681 gccgttgagc atgagggcat gactgccgat gccgatcgcc acgaaattgg cgcggcggcg
    13741 cacgtgcgtc gagttcagga tcgcgtcgcg cgtcgcctgc ggcaggctgt ccagcccctt
    13801 gcgcgccaga tcgataaacg ccttggcgtc gtcggacgag gcgaacaata tgtagtagaa
    13861 ctgcatgtcg atgcctgcgg tcaggaaccg ttcgacctcc atcgcgatgt gccggtcgaa
    13921 cagctcgatg gcctgcggca cgtcggccgg ctgaatgccc aggctgcgcg ggacgttcat
    13981 tttgaagaat tcgggcgtca gatcgctcgc tccgctggcg cgggcatcgg gaaaaatctc
    14041 gttggtcacg tagcccttgt tgcgctcgac ccagtccatg ctgcggtatt cgtaatccct
    14101 ggccaggaac gggcgataga cgtagttcgc gatcggcagc gacaccattt tgatcaccgg
    14161 catgctgtga aacgcggagt aggaatagaa gtaggcgggg atgacataga cgatcagcac
    14221 gatgatggcg gcgacgatcg tccacttcag gccggcgctg tgaatgaaat tgtagaccgc
    14281 cgcgacgatc cgttccttgc ggcggcgcgc cttgaacgcc gcctcgctga tcccggccag
    14341 ccgctgcttc tcttcctcca ggtgcgcgat ctgctcggtc agcaggatgt agcattcgtt
    14401 ccagtcgaat tcgaacttct gacgttcggc gtcgttatag ttggccacgc gctgcagcag
    14461 gatgcagtcg ccgacgatcg tcatgatgtt ctgaccggtc ttgtggctat gctccagcgt
    14521 ggccatgaag ctgtcctgca gcatcgcgat gcggctcttc agctcgagga atttcatttc
    14581 ggcctggggg gtggcgcggc ggctcttgac ggcggtcagc acgaaatcgc ggaactgggt
    14641 ccaacgcgtg tggagctcct tgatttcctc caggttgcga ttcaggacgg gatcttccat
    14701 ggccaatgtt ccaagtcgga atgcgacgcc gggagagcgc cgtgcggcgg accgccgccc
    14761 gcgacagggg gcaagctgtg cgttccgtcc gggctgccag aatcatagcc ccggcgaccg
    14821 atggggtcaa tccgcacccg ccggtccgcg tcaaccgcgc agcagcgact gcgccccatc
    14881 gatccagatt tcggttcccg tgatgtgact ggaggcatcc gacgccagaa acaggaccag
    14941 ccgggccacc tggtgcggat cgcccggttt gccgccactg agcgggatcg ggttttctgg
    15001 aaatgcgagc ggcggcgcgg cctcgtccgc atcgcgcatt tcggtgctgc tgtcgatgtc
    15061 cgtcgcgatc gcgccgggac agatcacatt gacccgcacg ccgtggcgcg ccgactccag
    15121 cgccacgaac ttgcacagcg ccacctgcgc cgccttggag cagccgtagg cggtcgcccc
    15181 ctgcgaactg aagacgcgcg tgccgttgat cgacgaggtg atgatgatcg atccgccctg
    15241 gcgtttcaga tggggcatcg ccgcgcgcag cgtcaggaac gtgccgcgca ggttgatgtt
    15301 gagcgtcgtg tcccattcct cgggctcgat ttcttcaatg ggggcccaga cgccgttgat
    15361 gccggcgttg gcgaagacga tgtcgaggcg tccccagcgt tcgaccacct ggcggacggc
    15421 gcgctcgacg tcttccaccc gggcgatgtc gccggcgacg gcgaacgcct cgccgccccc
    15481 ttcctcaatc tcctggaccg tctcctccag gtcgcccagg tcgcggctgg ttacgcagac
    15541 ccgggctccc tcggcggcga gcaggacggc ggcggccttg ccgatgcccg agcctccgcc
    15601 ggtgatgtag gcgacgcggt tttcgagttg catggggtgc ctccggtcgt gccggttccg
    15661 cggcgagggg cgcgagggcc gtgattcacc gtcaggttgt ccggaagcaa ccgtcaagcc
    15721 agttggggag taccgggtgg atcagcgtgg cgatccatcc gatttggata cgattccgat
    15781 accgatgatg cttgcgctcg gggatctaat ccagtttcag atcgtcgggg acgtcgccga
    15841 ggagttcgtc gagatcgagc gcgatcggcc cgtcggcatc ggccggcggc ggcagctcgg
    15901 gtttcttctc cttgtgatac gcgatgcggc gggcgcgata ttcctcggcc gacatcgcca
    15961 ggcgcggctc ctcgccccgc tccagttgct cgaagatctg ttgcagatac gggacgcacc
    16021 agccgcagcc ggtgcccgcg ccgtggcatt cggcgaactg cgaggccacg cgcgcgcggc
    16081 gccgttcgtg gaaccggcgc agtttgccca gcgggacgtg aaagcagagg cagacttcgc
    16141 gatcttcgga tggcgccatg atgaaccttg tgggcgtgtt tggccgggtc gccgcgcgcc
    16201 gcccagagtg cggccccctc tactataaca gcgcaaccgc ccgcacacga ttcccgaaac
    16261 cgcgattgtg aaggcggaga catggcaccc cgcttgctct gagagcgagc aaacgacggg
    16321 gaggaggctt tttttctatc tgatcgccgg ggattgacgc gcgccatcgc ggcggcttcg
    16381 atgcccggac cgcggcggcg aggagcggaa tgggcggtga cagtgttgaa ttcgaagcgg
    16441 ggttggatca cgatagtgag accgatgacg atcccgattc cgatagcgat cccgatcccg
    16501 atagcgattt tggaaaaaaa taccaactga gaggagttga gcgatggacc tgacactcaa
    16561 ccggcgcgac atcctgacgc ggaccctgcc ggccgcggcg ttcgtgggcg cgagcttggg
    16621 ctcgctcggc acgctgatgg cgcagccgcc ggcgcagggc cagatgggcc agaagtcgat
    16681 catcggcagc atgatcatgc aggcggccgg cgacggccaa tacaagttgc cgccgctgcc
    16741 ctacgattac gacgccctcg atccgcacat cgacgcccag acgatgcagt tgcaccatga
    16801 caagcaccac cagggttacg tggacgggct gaacaaggcg gtgcagggga tgacggagat
    16861 ggcgtcgggc gagatcgacg atgcgaagct ggccgcgctg gaacgcaacc tgagcttcaa
    16921 cggcggcgga catctgctgc acacgatctt ctgggccacg atgtcgcccg acggcggcgg
    16981 cgcccccgag ggcgaactgg cggaggcgat caatgccggc ttcggctcgg tcgacggctt
    17041 caggaattac ttcagcaagg cggccggcgg cgtcaagggg agcggctggg gcttgctcgc
    17101 ctatgaaccg ctcggcgacc gattgattgt gtttgcgctc aacgagcacg ataccaaact
    17161 gatggccggg gcgtgcccgc tgctgccgct cgatgtgtgg gagcacgcct attacctcaa
    17221 gtatcagaac cggcgggcgg actacatcaa ggcgtggtgg aacgtcgtca actggaaggc
    17281 ggtcggcgag gcctacgcct gggcgcgcaa ggcgttccat ggccgcaagg gcgaaggcga
    17341 atagctgaag aacatgggat ggagcccaga gcaggatgcc ctcctggagt gcggcggcag
    17401 aaggcgcgca gcgcttggcg acaccgcttt ggatcgcaga atcagccagg gtgatcggat
    17461 ccaaagcggt gtcgacgcgt tgcgcggcaa cgctctggca ccgcactccg agaaaaccgc
    17521 ctcgaccccg catccttgca cccgcaaccg gttcgctcgg cacgattatt gtatgggagt
    17581 cgtccaacct tgtttggagg actcccatgg gaatcgtgac cgacgccacc gacaagcatc
    17641 aggcatgcat cgacgcgtgc cagacgtgca gccaggcctg ctatgagtgt ttccgtgcct
    17701 gcctgaagga accggatgtc gcgcaacgca ccgagtgcat cgcgctgctg gtcgaatgcg
    17761 cgatgatgtg ccagacctcg gtggcgcaca tggcgatgag cggccggttc agcgccgacc
    17821 attgccgcca ctgcgccgcg gtgtgcgagg cgtgcgccga ggaatgcggc catcaccggg
    17881 acgagcattg ccccaagtgc gcggaggtct gccggcgctg cgccgccgaa tgccgccgaa
    17941 tggccgcgtg aggccgccgc gtgccacgcc ggcgagcacc cggtcgaggt ttcgccgcca
    18001 attcggcttg cccgcccgcg cggtgcgcgg ataatgggcg aagaagcccg gatgatgaaa
    18061 ggcgtgacgc ccatgccatg cgagatcacc acgggccgtt gctggctgtt ccgtttgcgg
    18121 cggggcgaaa ccgggcctgc ccggccgttg cccgcgccgc cccggcgcgc cagccggccc
    18181 cggcccagcg gccatcgcgg ccagagccgc gcctgcgcgc ggcgcgagct tcgaccggcg
    18241 cggcggcgag ccgcgcggta aacgcgcggg agaaacggtc agcgcggcgc cgcgtcgagc
    18301 gtctcgcgca cgaaccgcag caggtccttc ggattatagg gtttctggat cagatgggga
    18361 tcgccgtcgg ggatcaggtg atcgtgcaga ccgtgcgtgc tgtagccggt gctgaaggca
    18421 acgcgcacgc cgggccggat cgcgctgatc cgttcaaacg ccgcgcgccc gtgcagccgc
    18481 ggcatgatca cgtccaatag caccagatcg atctggtcgg cgtgcgcggc gaacgtggcc
    18541 accgcctcct cgccgtcgca ggcggtcagc acgcgatagc cggcgccgcg cagaatgcgg
    18601 accgacaggt cgcggacagt ctcggaatcc tcggccagca ggatcgtctc gttgccgccg
    18661 acgacgtcgc ccatttccct gaggcccagc tcggcggcgc tgcggttgac cagcggcaga
    18721 aagatcttaa acgacgtgcc gtgccccggc tcgctgtaca ggctgatgaa cccgccgtgc
    18781 tgcttgatga tgccgtaaac ggtcgccagt cccagccccg tcccctcccc ctcgggtttg
    18841 gtggagaaga acggctcgaa aatccggctc tgggtcgctt cgtccatccc gcagccggtg
    18901 tcggtcacgg tcagcatcac gtagggccct tcgctggccc agggatggct ggtcttgtac
    18961 gccgagctga tgtcggcgtt gctggtctgg atcaggatcg agccgccgtc gggcatcgcg
    19021 tcgcgcgcat tgagcaccag attcatgatc acctgctcga tctggcccgg atcgacgctc
    19081 actgtcccga ggttctccgc gggccggaac tgcagggtaa cgtgctcgcc gatgacccgc
    19141 tcgagcatcc gcaggaaccc gccgaccaac tgattgagat caacgtcctt gggctggagg
    19201 atctggcgcc ggctgaaggc gagaagctgg cgggtcagcg tggcggcctt ctggccggcg
    19261 ttgatcgatt gctgcaagtc ggcgcggacg ggcgcgtccg gcgggagcgt ctccatcgcc
    19321 acctgggtgt agccgatgat cgcctgcagc aggttgttga aatcatgggc gacgccgccg
    19381 gcaagctggc cgatcgcctc catcttctgc gactggcgca ggcttttctc gaggattttg
    19441 cgttcggtca cgtcgctgat gtaactgatg acgccgcgaa agccgccggc cgaatcgatc
    19501 agcggcgcgc tgtaaagctc cagatcaacc cgacggttga cgtcggcgcg cgtgcagatc
    19561 tgcatttcga ggaaggtcgg gatctgatcc ggaccggtgg ggcagagcag gaaatggagc
    19621 gccagctcgt cgcccgaccc gataaacggc gggggctggc cgaggacttc ctcgcgcgtc
    19681 cagccgaaca gtttttcggc cgattcgttc cagacggtga cccggccctc gcgatcgacc
    19741 gcgacgatcg ccatcggcga cgcggcgatc agcccctcca tctcgtgata ggtctcgcgc
    19801 agcgagttct cgacgcgggc gatttcgcgg cgctgctcga cctcgttcag cgcgcggcgg
    19861 atcatcggca acagccggcc gagccgaccc ttcatcagga agtcgttcgc cccctgcttg
    19921 agcgcctcga cggccagctc ctcgccgatc gtccccgaga tcaggatgaa cggggtgtcc
    19981 aggccgcgat tgcgcacgcg gcgcagggca tccaggccgg tgaaggcggg catggcgtaa
    20041 tcggcgagga tgacctgcca ctcgccgtcg gccagagcct ggtcgaactg ggccgccgag
    20101 tcgaccgcga tgtaatccgg atcatatccg ccgcgacgaa tctcgcgcag caggagcaag
    20161 acgtcatcgg gggaatcttc ggctatcagg actcgcagcg gagtgcccac ggtgctcact
    20221 ttgcctgggg aggtcgcgtg ttgatcagga gccagtacag cccgagggtc tggacggcgt
    20281 tgagaaattc gctgaactcg accggcttgc ggacgtagct gttcgcgccg cggctgtagc
    20341 tttccaccag gtcgcgctct tcgttggagg aggtcaacac aatcaccggc atcagtttcg
    20401 tttcgggatg cgagcgcaat tccttgagga attccaggcc gttcatccgc ggcaactgca
    20461 agtcgagcag gatgatggtc ggctgctgcg aggcgtcgcg gccgctgtag cggccccggc
    20521 cgtggaggta atcgagggcc tcgacgccat cctcggcgac gacgatctcg ttgtggatgt
    20581 tctgcttgcg aaaggcgcgc agggtcaggg cgacgtcgtc cgggttatcc tcgaccagca
    20641 atatcgtctt gtcatccggc atggatggaa tcctccttgg gcaaggtaaa gtacatgcgc
    20701 gtcccctggc cggggatgct ctcggcccag acccgtcccc cgtggcgctg aaccacgcgg
    20761 cggacgatgg ccagaccgat cccggtcccc tcgaaatcct ggggccgatg cagccgctgg
    20821 aacgggctga acagtttgtg gacgtactcc atatcgaagc cgacgccgtt gtcggtgacg
    20881 cagaagatcc gctcgccgtt ctgctcggcc acctgcgaga cctggatgcg gggagcctcg
    20941 accggccgcg tgaatttgaa ggcgttgtcg atcagattga ccagtgcgat ccggatcagc
    21001 cggcggtcgc ctttcgccgt cagttgcggc tggatttcca cggcgacgcc gtcatactcg
    21061 ccgccgcggc ggcattcctc ggccacggcc tgcgcctccg cgctcaggtc cagttcgtcc
    21121 ttgcggactt cctcgcggct gacgcgcgag aggttgagca ggtcatcgat cagcaccccc
    21181 atgcgtttgc tggccgcgac gacccgccgc agatagtcgc actcttgctc ctccaggcgc
    21241 gggcccagat cctccagcag ggcctcgctg aagccggaga tgctgcgcag cggggcgcgc
    21301 aggtcatgcg aaaccgagta gcagaacgcc tccagctcct ggttgacctc ggtcagccgg
    21361 cgcgttcgtt cctcgacgcg ccgctccaga tcccgggtcc gttcggcgcg ccccttcaac
    21421 tcgcgggtca gcagcacgaa acaccagatc agcaggagca cgacggcaac agcccccccc
    21481 agcagcgaga tcagcatgaa ctgggagtag tgcgaggcgc ggtgcaggcg gctgtccagc
    21541 cggttgcgtt catcgcggat cagcccgccg atcagcgcgc ggaccgagtc catctcccgt
    21601 ttgccgtagc cgagtcgcac gtgggcgcgg gcggggtcca ggccctccgc ctcgaagatg
    21661 ttcagattct cctcgagcgt gatggtgcgg gtggaggcgg ccgcccgcag tttgagcaga
    21721 ttctgataat gctcgggatt gttgcggctg gtgcgcaggg cctggtcgat ctggccgtca
    21781 agctgccgcc ggccgcgcag gtagggctcc aggaagtcgt ggtcgccggt cagcagatag
    21841 ccgcgctggc cggtttccat atcctgcagg gaggagagga tcgcctgcaa ctggagcagg
    21901 accaggtagg attgggagat ttcggcgtgg aacagggtct gtcggcgcgt gacgaggatg
    21961 ccggcgatga catcgacaac gaggactgcg atggcgaggg tcatccagag gcgatggcgg
    22021 cgcatgccgg agcggggcat gaacaaaatc cctacaaagg cattaagttt cgccagtata
    22081 gagggtgatt gaatcaggat caattaaaac ataagggcaa tcaagccaac ccgccgcgtg
    22141 tgagtcccgt ccggctcgga agcgtgccgg aagggagctg tggagcgagc gggaaggggg
    22201 tgcgggcgag cggcgtagtg ggggacaaat caactcgttg ataacggaac gattatgaac
    22261 cggccagggc gcgggacccc tttgggaaat tcctccgcgt ctcggcgtct cggcgtgaga
    22321 tctgcgcgcg tccaggacgg aatgacggcc gacgatccct gcgcggcgct taaccggacg
    22381 gccatgaatc aaaagacaaa caatctcacg ccgagacgcg gagacgccga ggcgtggaag
    22441 gcgaagggat gaagcggaga caagataggt cggccatgaa ccgggggtca taacaaggga
    22501 tgaaaaggga gggcgaggcc actcaggaga gtgacgctcc cagcttccgg ccgaatttcg
    22561 agagccggga gccaaatctt gggtgggcct tcgaaggacg aagacgcggg gcgatcaggt
    22621 ggagacggcc cgttcgatgt cggcgatgac gatctttttc atctcgaaca tggcgcgcat
    22681 ggcggcggcg gcacggtcgc ggtcgggacc cgagaccagt tcgagcagcc gcgacggcgt
    22741 gatctgccag gccaggccat agcgatcctt cagccagccg cattggatct cggtcccgcc
    22801 gtcggtcagc cggccccaca gcgtgtcgat ctcctcttgc gtctcgcatt cgaccagcag
    22861 cgaaatcgat tcattaaacg gctggttcgg ccccccgttc atcgcgtaga agcgctggcc
    22921 ggccagctcg aacgtcacga gcatgaccgt tccctcccgg tccggattgg cggcggtgta
    22981 gcgcgcgaca tcgatgatgc ggctgtccgc gaagatcgag gtgtagaacc gcgccgcttc
    23041 ctcggcctgg ttgtcgaacc acagaaaggg ggtgattttc tggcggatcc ggggcatggg
    23101 aaactccttg cggttcagga cggttcgaaa gctcgccgac gcggcgtgcc ggcggggcgc
    23161 ggaaaagata cggggcttga cgtcgtggcc cggcgccgcg gcttgacgcc gcaatccgtg
    23221 cgggccaacg ccgggatttt accatgccta tccggcgggg ggaagcgata cccgatggcg
    23281 agggcgaagg cagtcgcgat gccgagctga tcccggtcgc gaggacgagg gacgaggacg
    23341 agattctgtt gggcgggtcg ggttactgga gggtggtcat gatatggggg agcatttcgc
    23401 tcgcgagggc gaggatgcgg gcttcggctt ccgcttcgtc ttcaccgatc gcggtggaaa
    23461 accggatcag cgccgagacg gtcggctgca tccggagctg gttgtagacg gtcttgagct
    23521 gctggttgta gtaattgggc gtcagttcgt cgccggcctt gtagcagtag aggatcagat
    23581 cgtcgcggcc gccgtagctc atcttgacgc gcatcatcgg gggaaggccg gccggctcga
    23641 tcacgcgctt gtccatcgcc tcccagccgg cggcgcggta gcagacctcc ggcgggtggg
    23701 ccacgcggcg gttatcgggc gaaaagacga cggccaactg cacgggcacc gggctgagca
    23761 gcgggttgat gtagttgcgc tggatgacgt cgtcggtctc caggatctgc ttgacgtatt
    23821 cctcgaccgg aacgcgctgg ccataccagt cgccggtctc catcggcaga tgttcggcgg
    23881 tgtcggagcc gcgcacctgc gaggtgggga agagcatcag ggccgacccc ccggccccca
    23941 agcccatcag cacgaggctg atcgcgacgc ggacgcgcaa actacgcatg agtcgactcc
    24001 cgggggcggg cggggatcgg ctgggcgggc tctccggccg gccgcggcgg ggcatcttcg
    24061 acttcaccgg ccgagccgcc gccgccgtcg ccgcggttcc agcccagcgc ccgcgccgtt
    24121 gcatagagca gcacgaatgc aatgacgaaa atcatgtagc ccgagacatc gtgcaccgtc
    24181 ccctcgatcg cgcgatcgcc ccagcgatac gccaccatgc agaggaagaa gacgcgcagg
    24241 acgttggaga tcagcgcaat ggggatggac gcggcgaaca ggatcgcgcg gcgcgccggc
    24301 gtcaggtcgc tcaggtaagc gaacagcagg ccgaggaaga tcagcgagat cagcgagcgc
    24361 agaccggagc aggcgttgcc cacggtgacg gcggcgtcgc ccagcaggat cgtcgccccg
    24421 tcgttgatcg ccgtgatgcc gagcagcccg atcatcttga gcgcgagcgt cccggccagc
    24481 atcttcatcg caaagctgat tttttcaatg atcgccagcg gcagcggcac catgaacgtc
    24541 aggatgaagg cggcgaaggc gagccgcttg aagaccggcc agccgaacag gaagccgcag
    24601 aggccccaga cgacgaaaat catcgcgaac gcggcgggcg aaaagatcgt cagccagccg
    24661 aaaacgaaca gcatcagcag gccggcgagc atgacgaaca ggcccgcgcc gggcgtggcg
    24721 aaggggcggg ccgccagctc gaagcgatcg cgccagacga cgtagagcgc caccagcggg
    24781 acgatgaagc catgggagta gtaggactcg ggggcgaccc agcggtcaac catccaggcg
    24841 atcgggacgc ggaacgccag cacgaacagc gcggcgagcg ccgcgacggc ggtccagccg
    24901 gccgggctga tgcgctggag cgggtcctgc cggggcgagg tgaaagtcat gcgaagcggg
    24961 gcatccccgg tggtgcgttg ggtgagacgc ggcgagaagg cgccgcgcgc cgcaggtcgt
    25021 gcattcttct gagataacgt atcggtccgg agggcgtaaa gctaaatcgg gccgcgccga
    25081 cggccttatg ccagacggaa acgctgaacg aatcccgtga tcggttggat cggccggctc
    25141 agattgcgaa gccgccgccg gtcttgtcgc ccaggtcgac cacgtgcgcc tcgctggcga
    25201 tgcagacctt attgcggcca ccggccttgg ccgagtaaag agcgcgatcg gcggcgttga
    25261 tcatctcctc ggccgagcgg tagcccccgt cgatcgacgc gataccgacg ctgatcgtca
    25321 gcgtcagcgt gtgaccgtcg cgctcgaagg agtggtgctc gaccgcctcg cgcagccgtt
    25381 cggcctggat cagcgcgccc tcgcgggagg tctcgggcag gatgatggcg aattcctcgc
    25441 cgccgtagcg ggtgcagata tcgttgccgc gcaccttggt ctggatcagc gcggcgagtt
    25501 cgcgcaggac gaagtcgccc accggatggc cgtaggtatc gttgaagcgt ttgaaatgat
    25561 cgatgtcgaa catgacgagc gccaggcgcc ggccgtagcg aatcgcgcga tcgaactcct
    25621 gcttaatctc gacgtcgaag acaccgcggt tgctcaggcc ggtcagcggg tcctggctgg
    25681 ccagctggaa caggcgctgg tcgaccgcca gctccgactc atcgcgcagg aagaagccga
    25741 tgacggagga gccgatgagg atcttgtcgc gatcctggag cagatggtcg ctgacgcggg
    25801 tgccgttgac gaacgtgcca ttggtgctgt cgaggtcgtg gacgcgaacc agaggggaag
    25861 cgaccgggtc gttgaagttt tcatagacca tgcgcgcatg gcggcgcgag cagcgcgagt
    25921 cgtgcagcgc gatgtcgcac atcaggtcgc ggccgagaat cagttcgcgc ttgaggacgg
    25981 ggatgcggcg attggcttcg ttctcgtcca ggacgatcag caccggacgc tgcgcgcgat
    26041 tctggatcgt ctcctcgcac tgatcggcga cggcctcatc gaaaatggtc ttctccggtc
    26101 ctgcggtcat catctacccc tgctgctgac ggaaaggtcg ggctcgccaa tccacggtag
    26161 gggcgcatgg agagagggag ccctgtgttg ttcactatat cggacgggca tcgccagggg
    26221 attaacccgg ggttcccgat ttggacgggg ccggggagga ttataaaata ccaaggactc
    26281 tgaaaatctt ttttggcttg cgcgacgggg gcggtttgag taaattccgt atcgcgatat
    26341 tgagatatta aatatcgctt ttatctagaa tggagtcgtc ccatggcttg ctcgatgtat
    26401 tgcgaccagt gcgaacagac cttccgcggc agcgcctgca ccgaggtcgg cgtctgcggc
    26461 aaagatcccg acgtcgaatc gctgcagaag attctccttt atggacttaa aggcatggcg
    26521 gcctacaagc accacgcccg ccggctgggc aagaccgatc ccgaagtcga gtccttcatc
    26581 gaggaggcgc tcttcgccac cgtcaccaac gtcaactttg acccgccgag cctgctcaag
    26641 ctcgtcctgg aatgcggccg gatgaatctg cggacgatgc agctcctcaa cgacggccat
    26701 gtggaaacgt tcggcacccc ttcgccggtc gaggtgagtg aaggcattca ggaagggccc
    26761 gggatcctcg tgacggggca cgacctggtc gacctgcgcg acctgctgga gcaggccgcc
    26821 gcggccggcg tccacgtcta cacgcatggc gaaatgctgc cggcgcatat gtatcccggc
    26881 ctgcgcaagt acccgcacct gaagggtcac tacggcgatg cctggcagaa gcagcgcgat
    26941 gagttcgagc gtttcggcgg gccgatcgtg gccaccacca actgcgtcca gatcccgcgc
    27001 caggagaaca cctacctgga ccggttcttc tcgacgcgca cgacggcgat tcccggcggg
    27061 aggctgatcg aaggcaacga cttcggcccg gtaatccggc gcgcgctgga gatcggtccg
    27121 ctccggccga cgccgatccg gcgttcgacg atcggctttc actacacgac gctgctcgcg
    27181 gtcgcggaca aggtcgtcgc ggcggtcaag gcggggcagg tgcgccattt ctacctgatc
    27241 ggcggctgcg acggggccga gcccggccgc aactatttcg cgcactacgc gcaaaacaca
    27301 ccccccgatt cgctgatcct gacgctgggg tgcggcaagt tccgcatccg caaccatgat
    27361 tacggcacga tccggctggg cgatctggag gtgccgcggc tgctcgatat gggccagtgc
    27421 aacgacgcct acggcgcgat tcaggtggcg ctggcgctgg ccgaggcgtt ccagtgcggg
    27481 gtcaacgacc tgccgctgac gctggtgatt tcgtggttcg agcagaaggc ggtggcggtg
    27541 ctgttgacgc tgctgcatct gggcgtccgg ggcatccggc tggggccggc gctgccggcg
    27601 ttcgtgacgc ccaacgtgct ggcgatgctg caggagcatt acgacctgca gccaatcggc
    27661 caggacgcgg cggccgacgt ggcgcggacg ctggcggcgg tgtaaggccg agggggcgcc
    27721 ggcgcgcggc gacgcgggtc gcgcgaaatc tccgtttctc cgcgcctccg cgtgcgacgg
    27781 ggagagccgg accgcacgcg gaggcgcggc gacgccgaga gagggctttc gccgaccgga
    27841 gggacgcgct atgctggcga agcgattaca aattgctcat gaccctgcgg tttacatcga
    27901 gggatgaaaa tggggggtga acgacccagg gcttgccgga tctagcagca gcggagtccc
    27961 gtcagggacg gcagatttca gcccgctacg tcagtggcgg gaaccgggat accgatcgcg
    28021 accgagtccc gtaggggacg gcagaaccga tccaggcgat cccgcgaata gcggacaagt
    28081 tttcgagtga gacgatcacg aggacaaggg acgaggacga gagacgattc tagaatgagg
    28141 tgagcgtcat gcctgaaccg acatcccatg cagctct
//
LOCUS       DAOPCO010000025        27850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1871_length_27850_cov_5.661954, whole genome shotgun sequence.
ACCESSION   DAOPCO010000025 DAOPCO010000000
VERSION     DAOPCO010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 27850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27850
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1871_length_27850_cov_5.661954"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..191
                     /locus_tag="PLS90_03540"
     tRNA            116..191
                     /locus_tag="PLS90_03540"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:149..151,aa:Ile,seq:cat)
     gene            complement(762..1730)
                     /locus_tag="PLS90_03545"
     CDS             complement(762..1730)
                     /locus_tag="PLS90_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK01510.1"
                     /translation="MPRTVITGGAGFLGSHLVERFLAEGHEVVAIDNLLTGSLENIDH
                     IHSDQFRFIKQDVTEYLYITGPVDNVLHFASPASPNDYLRMPIQTLKVGALGTHKTLG
                     LAREKGARYLLASTSEVYGDPQVHPQPESYWGHVNPIGPRGVYDEAKRFAEAMTMAYN
                     RYHGVDTRIVRIFNTYGPRMRLDDGRVVPTFVDQALLGEPLTVFGDGSQTRSFTYVDD
                     LVEGIWRLLNSDEHEPINIGSQDEFSILQFAELIIQLTGGTSRIIYQDLPVDDPKTRR
                     PDITRARQKLGWEPRTPIEEGLRRTIEFFRQKMNQAPRQTRQRFEG"
     gene            complement(1851..3434)
                     /locus_tag="PLS90_03550"
     CDS             complement(1851..3434)
                     /locus_tag="PLS90_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPK01511.1"
                     /translation="MTRRQSPLHPRRGALERRILVLCTMLVIVGVLLMVKMPQHIAAM
                     EEQQQYDADFYQFAELFSEIYSQIRANYVEEVDKEKLFEGAINGMFMELDPHSSYLPP
                     VDQEMLTKDTEGEYSGVGLHITLDKNKILTVISPIAGSPAARVGVQPWDRIVEIEGKS
                     TENISLMEAVNKLTGPTGTEVKVKIWREGAPELLDFTIRREVIKVESVFSKIIDENIG
                     YLRITKFQDDTAEDVKKALTNFNQQGVEGVIVDLRFNAGGLLDRAVEICDFFLDKNQL
                     IVSIQGRHESNNRKYYALNDKLCDQWLVVLVNHGSASASEIFAGAMQDTRRGIIIGPK
                     DSTTFGKGSVQTISTLRHSLERDENGNPKPSGLRLTTALYYTPSGKSIHEKGIAPDFG
                     VELPEGHEVELFRHGLLGDPPQIEPGLEGTPALDDEITTGSLDESREGPTHDMIELKQ
                     GTPDENAPALPDADAPATGEGETPDGQQMLELLRHRRDNLSSEDQDATESGEFEDILL
                     NEAVKYLKAIVYFEKQKVA"
     gene            complement(3628..4920)
                     /locus_tag="PLS90_03555"
     CDS             complement(3628..4920)
                     /locus_tag="PLS90_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01512.1"
                     /translation="MLNSLRLTLRIIRLGITNVRAHKLRSTLTVLGIVFGVCSVISML
                     AIGEGASQDVQAQIRSLGSQNVLVQSLKPPPNPNAQSENNSRSMVLEYGLTYLDAERI
                     SETIPGIQTLVPIKSRPADLWFGSRKVQVKVLATVPWFIENHPMRVVRGRYLTELDLD
                     RRFNHIVLSDRVAKDLFLFEDPLGQSVKIANDTYRVVGIVDTNKGGAPVEGAEELASQ
                     TADCFVPITTERLRQGDTTFHTGQGSSSAEKVELSELIIQVDELDRVIEVANAVDALL
                     SKHHPKKDYRLVVPQRLLEQARRTQRVFSIVLGSIAAISLLVGGIGIMNIMLASVSER
                     TREIGVRRALGARRRDIMSQFLVECLILSLGGGLIGMALGAGIPLMVTKLSAMPTVLT
                     PFAFILSFAVSAIVGVVFGLYPARRAAMMDPIDALRHE"
     gene            complement(5015..6703)
                     /locus_tag="PLS90_03560"
     CDS             complement(5015..6703)
                     /locus_tag="PLS90_03560"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPK01513.1"
                     /translation="MNSYQTAERRRGGWTLRLISVVGLLAVVSVSLLAMRGLSKPEHD
                     AVGKDAGGELTYKVTRGPLKISLLAPGSIKARNSRSITCEISNAEAKIIWIIPEGTIV
                     EKGDKLVELEAAGLDDNLVKQRIDTATAQAAYDEAVEQLAIQESQNQSDLLAAQNKLE
                     LAELDLKKYRDGELQRLKAEKEAAISLAEAELKRAETRLEGTLMLQENEFVSRGEVEA
                     DELTVQKQRHELEKAKRDYDVLVKYEEERELSKLEQDVAAARGELERVQLSSRSELRT
                     KQIAVESAKAKLELEQSRLQTLEHQMANTVIYAPEAGMVVYANDGNSWRGNDDEIKEG
                     AQVRYRQKLIELPDFSSWIVETRVHESMIRQVEVGLPAIVMVDALPNQRLSALVEKIG
                     VLPNSGGRWFMPDVKEYNVDLAVTTTTLPLKPGMSARSEIVLAELDDVLYVPVQSVAT
                     SGNQAFVWLRKDLGFVKHPIEIGRNNESFVVVEAGLNEGDTIRLNAPDQGGGRRVIER
                     PSNSPEDAQGEAKPARSQERTAEAERDAGKPAADAPADSPLAHDSAAPAKVGEG"
     gene            complement(6681..8480)
                     /locus_tag="PLS90_03565"
     CDS             complement(6681..8480)
                     /locus_tag="PLS90_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK01514.1"
                     /translation="MLFSQFPSVASLIRIAGSLSICGLGLLLTGCFGNLAHRLADRET
                     YQIIGEKQEEALGARQPFTIEPDQSDRTRSIYAEAGRSADPFTTTGLVLSLSDTLALA
                     IDNNEDYQTAKEDLYLTALELTEERHQFSPIFTGLISARMNRVPATETRVSTEQVEVT
                     DPVTGMPTVVTRTRTEERTIAERFGEMQTSLAVTKLFATGARLTVGLTNNFLRFYTGD
                     KRHVDTGALSASIVQPLLQGAGTDVNLENLRQAERNVIYAVRDFSRFEKSFLIDRIDD
                     YFRLLQAYDQIDNEWRAYQRLTVLRRRAELFEEADRMAPFEVDQARQDEIRARNRWLT
                     ARTDYGILLDQFKVDLGLPPELTILPDERELDALRNAGLVELQYGLEEAQQVAVARRL
                     DLMTAQALAEDAARRLKVAENNLLPVLDARFDYVIRDSGENHPFDLTNELREYGAGLD
                     LELPLDRKVQRNDYRRAVITRALRERQADRLRNQVIAEVRRTYQQAVQAAQSYEIQLA
                     SRELAAKRVQSVDMLLDLGREGVSIRDRLEAEDALRNAENELTRELVNYLIARLEFYN
                     AIEALEIDERGAWHEMAPDTTTRNLDHEQLSNG"
     gene            complement(8665..9747)
                     /locus_tag="PLS90_03570"
     CDS             complement(8665..9747)
                     /locus_tag="PLS90_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPK01515.1"
                     /translation="MSLTEQAVREALARVNDPELNRDLVSLGMVRRIDIADGAIRLRL
                     ALTTPACPLKSVIERDVRAALEPLPGFRGVEIEFTADVAGRARGQQDLAPGVRNIVGI
                     ASGKGGVGKSTVALNLAVALSQSGARVGLLDADIYGPNIPTMMGLREPPRVVKNQIVP
                     LEKHGLKMISMGLLVPDDSPVVWRGPMLNSALRQFFGDVDWGELDYLLVDLPPGTGDV
                     QISLVQLVQVTGCVIVTTPQLVSTQDARRGLAMFQQTNTPVLGIIENMAWFRCDGCDK
                     IHRLFGEGGGERTARELGIDFLGSIPLGQNVREGGDSGVPVLLADPDCEQSLQFRRIA
                     GELAAAVSVQNLEGGRRPVEINLGSG"
     gene            10009..12096
                     /locus_tag="PLS90_03575"
     CDS             10009..12096
                     /locus_tag="PLS90_03575"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK01516.1"
                     /translation="MSLPRAFVRLSVWAGLMLLPVGAAGETLSYADLVHRLVDLERLA
                     LLPAEGEVCRQASSYDRASVYDESTGRYVNWSANNDGDGFIRQVNGRQVLAEIEGPGC
                     IWRIWSAAARGGRVRIYIDAQDNFESATVNLPFNGYFNRENEPFTYPGLVYQTPSGYG
                     YEGGYNSYVPIPFNQSCRIVADPGWGLYYHFTYSQFPEGTTVPSFKRDLPEEGQAALR
                     EVSDFFERRLGEDLGGPRENAETTVRAIEAAGGGAATRVAELSGPAAITALKVRMPEM
                     DRKTQQRVLRELVLRITWDGAEAPAVWCPLGDFFGTAPGMNEFRALPVGLIGQGFYCY
                     WYMPFATSARIEIVNDGAERRDLMFEIITAPLERPIESLGRFHAKWHRDAFIPEAETG
                     RAIDWPLVKTTGRGRFVGNLLNVWNPRGSWWGEGDEKFFVDGEKFPSTYGTGSEDYYG
                     YAWGLPLLFAKAFHSQTVAQPENRYHISVNRWHIADNVPFQTELEACIEKYYPNSRPT
                     MYSAVGYWYLDPAGTDPYGPVPAEERVDYYVMPEIFRIEGVIEAEDLPTSTGAGFARF
                     QDLTLWGQNAWSGDAHLWWTEARTGDRLSITFPVDTAGEYQLIASFTKAPDYGTVQAY
                     LDGQPVGEPIDLYISVWVEPTGPVDLGRHRLTAGEHTLTLQITGKNENAANTYVGLDY
                     LKLVATGTGAGDD"
     gene            complement(12109..15168)
                     /locus_tag="PLS90_03580"
     CDS             complement(12109..15168)
                     /locus_tag="PLS90_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HPK01517.1"
                     /translation="MNLRHASARPLAAGLISGCLLLCAAGLAHAAEDFYEENDTRETA
                     ADISAAAGVRLSSWNASYGYQNDEDWFAITVAPGNGRVQASIAFKNKEGNLSLDLFPA
                     AGEDWLTTSRSLEDYELIDFNVLFPGTYYLRVSGPDLGTQYDLWWNALPLAGDDQYEQ
                     SPNNSIATAVNATGFAGHWLTNLDGPGILRDVDYYRINPTDDATRVVIDARFHHSEGD
                     LDLQLLDGAGNILAQSLGTGDRELINFVVPAAGSYYIRVIAVSYSGAQDYNLYWNRYP
                     AADDAYEHNNGVSEAFDITPHGTLHLSTLRGQGIQADEDTFAIHLSNAPLRLLVTLRF
                     KHAAGNMGLQLFDPQGLPLAVVDGTADFEILDVLISDPGLYVVRVFGEDRGNTYDLWW
                     GATAEAGDDFYEDNDTQPFSRTLVTTPGQWLTDLKGPGIIRDVDFYTVSTNAPDQWLH
                     VVCLFDHDQQDIDLQLFDSMGTPLAESVSRTDFESLHVQLPNQGNYFVRVFHFGPGGG
                     TYNLLWNRESGNDDAYENNDSAAAACNLSPFMGQRLSRIMGPGRLGDLDYFTFDVPEG
                     MLTLFLRLTYDFFAGDVNWRLYDPDQMLIDEGTNQSNEETARLTLPKPGRYTIQFYGQ
                     YMGNPYDFVYRFGPTGDDTFEQNDTRLGAFNLAPHAGEYLTQIGGLGIQLDDEWFRIQ
                     MPDGLTHLFAQLISIAADGNLDLELYGIGGNLVDASRANSNYDLLDFELPQPGEYWLR
                     VVGNNSGQVYDLRHYTLAPGDDLYEENDTPQQATNLKFLAGTYLSLINGFGYQTDDDW
                     FTVETGPNDQRLVVEVLFKHDLGNIDLEVYDSTGQTLLGQQISLTDNEGIDLPISPNT
                     EYKLRLYGANVATARYNLWWDGMPPHPSGDDNYEQNDSPNSAANLAQAEGQWLSTFDG
                     GARWLDRDYYLLPVPAGRERVVVHLRHDLENIDAVLRLFDPEGALVAQHQSSADTLVL
                     GHLVDGPGDYILEVAPVTGLDTFNYDLIWWAVERGNAVGGPAWAVYE"
     gene            15784..16614
                     /locus_tag="PLS90_03585"
     CDS             15784..16614
                     /locus_tag="PLS90_03585"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPK01518.1"
                     /translation="MGGFILNFTPNGMVPTREMTPHVPLTPEEIVRDVDDCVRLGANM
                     VHLHARDAEGRPTYDKEIYARIIGGVRERHPELLICVSTSGRLHNEFWQRAQVLELEG
                     DVKPDFASLTLSSLNFARAASINEPAMIQDLARRMLARGIRPELECFDLGMVNVAQYL
                     IDKQLVPEPYYVNLIVGNLAGAQADLISVGALRAALPAGAYWSVGGIGHSQLVANTLG
                     LAAGGGVRVGLEDNLWLNEGRTTLASNAALVERVVRIGGLLGREPYSPAEVRALLTNP
                     "
     gene            16680..17372
                     /locus_tag="PLS90_03590"
     CDS             16680..17372
                     /locus_tag="PLS90_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01519.1"
                     /translation="MAQADPSPRSTPAPGGASVWRKLRDVIRHGLVIQTLLDVLRKAG
                     LEITPYYVMREAVVSPDAAPVAGLENLRLAWLGPEDAAQVGALPDSKFSEAETRARLA
                     RGQRCLGAWVGSELAAFMWVDTGELDFEPCRGPLHPHEAYLFGATTTRARRGHNLVPH
                     LRAELYRALRAEGRTVCYSISYAFNTPALRFKRKLNARCDRLYLYVSLWGLARRNWLL
                     RRYPAAPGDGEP"
     gene            17459..18631
                     /locus_tag="PLS90_03595"
     CDS             17459..18631
                     /locus_tag="PLS90_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01520.1"
                     /translation="MLGPLMLLVTSYTGDSDQLIVGDGREYFEYARVLVIEGHLPYEH
                     LRYPCGVSLIGIIGYAPSVWLARAAGWLERGRAAEGWAIFNQFSFCAPLILLAAAGVW
                     ANLRMLARLGFAGPVARFSLMCWLVGTNLPWYLFKEPAMSESATFSTLSIYYYLLIRD
                     GYGNVSARPDPRRWAVIGFFLGLAGAIRQQNILHCAAVPLLVVLQQRGVWREQPRQAV
                     RNVVWCVAPAAAASAVPFVVPWIVWSFAREGARLYAYGSEGFDFLHPRLLEILILPGY
                     HGFFAWTPMFALAALGWIFFFRRHRGLFAPFLVPAFLQYYLIASWWAPSFGASIGHRG
                     FITILPVLLPGLAALLEWSRARGGRVERALAALLLALIAANGVVLLMLATGRLSAN"
     gene            complement(18664..19116)
                     /locus_tag="PLS90_03600"
     CDS             complement(18664..19116)
                     /locus_tag="PLS90_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01521.1"
                     /translation="MTPVNNRPAGNAGESAIWLVHWAMTASVPFFLLLAWFVMFPQIS
                     TPDAAAAPAQRTAALLLIVVAVAQIPAGFMLPALLWNLTASRGLARVKLMAILSDALF
                     ESIAMFGLVGPYVGMSQMMGYGLMLLATVMLGMNTARIRVWIEMAARE"
     gene            complement(19113..20024)
                     /locus_tag="PLS90_03605"
     CDS             complement(19113..20024)
                     /locus_tag="PLS90_03605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPK01522.1"
                     /translation="MPSIKHHLLNYLQFLKESGCLYLEGPLPALTFATNPGPARKSSP
                     PAPSGGSLQPGPMASAARPARPARSAQPIPSAPPDLFAPTATPSRPTLDPAFGPPLPR
                     EQRLDRMREAIARAEACRACALGATRKRLVYGDGSPEASIMFVGEAPGGDEDAQGIPF
                     VGRAGQLLNKMLAAIGFQREEVYICNTLKCRPPDNRDPLPNEKAACEHFLVEQLALVQ
                     PKILVALGAHAAQYLCRSDLPIGRLRGHWHSYHGIPLLATYHPAFLLRSPGFKPRAWE
                     DFQMIHAKYAELRPDDPRKIWRKEADG"
     gene            complement(20128..20820)
                     /locus_tag="PLS90_03610"
     CDS             complement(20128..20820)
                     /locus_tag="PLS90_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01523.1"
                     /translation="MNALALPAALALLCALAAPLPAPAQPAAPRAPSAEWLEEKGAEL
                     RARSLLRDQAGRVAEALELRELAGAQAVQAPPGAAALRLRNDMDRLRPLWILEPVDVD
                     SAADATDRTVRLPQPVKGDQWLALAAGRWRLRTFAGAPAGPNGVEWPPLTLELRGGRA
                     YAIDLTDQLEQTLWQSIRDRRRREELQRVNRQLPTDQPAATPPAPRLAPAPRAPAAEP
                     ESPSVTVEIQRD"
     gene            complement(20817..22235)
                     /locus_tag="PLS90_03615"
     CDS             complement(20817..22235)
                     /locus_tag="PLS90_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HPK01524.1"
                     /translation="MLIDSHAHLQDKKFARDLNRVLARAADAGIERLIVVGDKLESSR
                     RAIQLARHEPCLAAAVGIHPHHEAAFSPLALLELERLARDSAVVAIGEIGLDFHYPGY
                     QRERQIECFAAQAHLAGRRNLPLIIHCRDAYDDLIDLIRTDERIPRRGVVHCFSGDLP
                     QAEELIRLGFHLGLGGAITYPNGAALRDVVSRIGLDRVLCETDAPYLPPQSKRGRRNE
                     PSYMKHTVKMLADLTGVSYQDAARITKTNAIRLFDLPDAARGAVTYAIRRNLYLSITN
                     RCSNECYYCQRCQDYMVMGHFLKLDEEPTAAELLDRVADIHQYDEIVLSGLGEPTMRW
                     DVCRQVARGLKERGARVGLNTNGNGSLINQRDLTPELAGLFDSVGVNLIAHDRETYNR
                     IAHPRDPARSWDAMLDFVKSARAVVPDVTLNLVAVPEVDVEACRRLAEDELQVRFRVR
                     EWRGNGDAGCAVCAPDPGAGAS"
     gene            22510..23823
                     /locus_tag="PLS90_03620"
     CDS             22510..23823
                     /locus_tag="PLS90_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010321637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK01525.1"
                     /translation="MNAHLQISRFVGLAGLMLLAATAGARQVALEVQAGQPLLKAGEK
                     QTAYLMITLTGPAVESASERPPANVAIVLDKSGSMGGEKIAEAREAALLALERLDKED
                     IVSIVAYDTDVRVVLPATRLRDREQIETAIRGIEAGGNTALYGGVSRGAEELRKFQER
                     DQVNRLILLSDGLANVGPATPEELAALGRELGGEGLPVTTIGLGLGYNEDLMYRLAEQ
                     SDGNHVFAERASDLASLFDEEFGSILQVVARDLRVAVRCGEGVRPVRVLGRDAEIDGR
                     DVMLELAQLHGARDTDIILEVEVPAREAGDSLKLAEVEVEYRNLLNETDERLTSEVSA
                     RFTDSSEAIEKAENDEVMARVVQRIAAERNALATKLRDQGEIDKAREILITNEAYLKE
                     GAARYDAPGLGAYAEIQAGDAANLEGENWERQRKSMRSTQYQMFR"
     gene            complement(24225..24419)
                     /locus_tag="PLS90_03625"
     CDS             complement(24225..24419)
                     /locus_tag="PLS90_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01526.1"
                     /translation="MTTLAESDIAPKDSLVTGERRIAVSSLSRENREVWPWFAFAALG
                     LLTLEWHIYCRRAWLCRRTP"
     gene            complement(24537..25253)
                     /locus_tag="PLS90_03630"
     CDS             complement(24537..25253)
                     /locus_tag="PLS90_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01527.1"
                     /translation="MTRKPLTPEERLKLCRQLGISGLDHVHRMVVEGKSRGAHWLLNL
                     GLGARHLELFGYTAEGMERLGYDRRTLAQLGYAVQSAAPAPATVGGRSPAAGFSPAAP
                     HDAPAGRGPEQLRRLIAAGLRASELHSQGYTIHDCKRLGLSAMELAQLNFPIEALATV
                     CTAAELRRANFGARDLRTFFSGSELKQAGFRADEMRMAGFSVRDLLNLGYNENHIRTA
                     GYSTNELLREGLTTTTRKLY"
     gene            complement(25537..25896)
                     /locus_tag="PLS90_03635"
     CDS             complement(25537..25896)
                     /locus_tag="PLS90_03635"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPK01528.1"
                     /translation="MIAAGQVGIPDMMKLDVQGFELEVLKGARQALGITEVIFMEVSL
                     LKLMGPRLPILHDIVAFMHAAGYVVFDIVGFYRRRRDHALAQTDMVFCRENSPLRRQE
                     PLRNDFDWDWGNYLDKT"
     gene            complement(25915..26190)
                     /locus_tag="PLS90_03640"
     CDS             complement(25915..26190)
                     /locus_tag="PLS90_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPK01529.1"
                     /translation="MLDVSAHKGYWSGTFRRVFPQTEVVMIEPLVEMEPHLEQTCRKL
                     GNARYYLNGAGAEPGELTFTIWPTLSGSSFIPQPDQPGYESHEKRKV"
     gene            26528..27631
                     /locus_tag="PLS90_03645"
     CDS             26528..27631
                     /locus_tag="PLS90_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK01530.1"
                     /translation="MAKQPTSLGAFNGLGLHLGNLSRVSRARTRSISPENFSGEKGKG
                     GMATEGTGAECARDLGRGWKISPSVRIEPGTTFTMADIAGAGAIQQIWLTPTGNWRFS
                     ILRIYWDDAEQPSVECPIGDFFACGWGRFAPLSSLAVCVNPGSAFNCYWEMPFRKRCR
                     MTLTNLDDNPMVLYYQINYTLTDVPEDAAYFHAQFRRANPLPYKEVYTILDGVEGWGQ
                     YVGTYMAWGVNNNGWWGEGEIKFYIDGDGEYPTICGTGTEDYFCGSYNFENQTTHQYQ
                     EFCTPYAGLAQVIRPDGLYASQQRFGLYRWHIPDPIRFESDLRVTIQALGWRSHRRYL
                     PLQDDIASVAYWYQTPIAKKFPPLPGRDELEVI"
BASE COUNT         4604 a         7694 c        10563 g         4989 t
ORIGIN      
        1 taaccgggcg gggcccggcc gacacggccg aggggaagct tgatcggtgg tggagaggac
       61 cggtagactg gatggggtta caagggttgg gccttgccgg ttggcgcggc ccggtgggcc
      121 cttagctcag cggtcagagc cgccggctca taaccggtag gtcccaggtt cgaatcctgg
      181 agggcccacc aatcgtttcg ttctttgtcc atggagacgg gacggcgtgg attcgcgcca
      241 tgcggggacg gggttcgaag aaacatcggt ttgtgccgat gaatgacggc gttggggatg
      301 ttggttatcg aaccgctgac gcggttccta atttaatcgg atcggtgcag tcgtcgtgac
      361 atctcctgtc aggaggattc caccgaaaga ccccgtcggg gctgcaataa cgggataaat
      421 tgaccggtcc ggtgctgacc cgaagggcta ccgggtagca gcggacgcaa gcatctccgg
      481 tcgccccttc caggggcttg gatcgtaggg gcggcgacgc agggttccgg ccgtcggctt
      541 ccaccctgcg ctttcttccg gtcgtccgct tcgcggactg atgcggacta tatgttcgaa
      601 tcgcttggaa tcatgggctt ggaatgcttg agccgggcgc aatttgggga ctggcaagca
      661 tgcaacccac ggcttggata tcgaaccgct gacgcggtgc gtgatgcgtg atttgatcgg
      721 cgacgttgat ttcgaggggt gggggggaat tcgcaagcga gctagccctc gaagcgctgg
      781 cgggtctggc gcggggcctg gttcattttc tggcggaaga actcgatcgt gcggcgcagg
      841 ccttcctcga tcggcgtgcg gggctcccaa ccgagtttct ggcgcgcccg ggtgatatcg
      901 ggccgccggg tcttggggtc gtcaacggga aggtcctgat agatgatccg gctggttccg
      961 ccggtcagct gaatgatcaa ctcggcaaac tggaggatgc tgaattcatc ctgcgagccg
     1021 atattgatcg gttcgtgttc gtcgctgttg agcaggcgcc agatgccttc gacgaggtcg
     1081 tcgacatagg tgaaggagcg ggtctgcgag ccgtcgccga agaccgtcag cggctcgccg
     1141 aggagggcct gatcgacaaa ggtggggacg acgcggccgt catcgaggcg catgcgcggg
     1201 ccgtaggtgt tgaagatgcg gacgatgcgc gtgtcgacgc cgtgatagcg gttgtaggcc
     1261 atcgtcattg cctcggcgaa gcgcttggct tcgtcgtaga cgccgcgcgg accgatagga
     1321 ttgacatggc cccagtagct ttcgggctgg gggtggacct gggggtcgcc atagacttcg
     1381 gaggtggagg cgaggagata acgggcgccc ttctcgcggg cgaggccgag ggtcttgtgg
     1441 gtaccgaggg cgccgacctt gagcgtctgg atcggcatgc gcagataatc gttgggcgag
     1501 gcgggcgagg cgaagtgcag gacgttgtcg acggggccgg tgatgtagag atactcggtg
     1561 acgtcctgct tgatgaagcg gaactggtcc gagtggatgt gatcgatgtt ttcgaggctg
     1621 ccggtcagca ggttgtcgat ggcgacgact tcgtggcctt cggcgaggaa acgctcgacc
     1681 agatgcgagc ccagaaaacc ggcgccgccg gtgatgaccg tgcgaggcat cgaacaggtt
     1741 cccttccgca accgacaatg gcggcgcgcc cgcggcggac tgcccggcac ggcgccggcg
     1801 gctccaggga accgcgggcg ccagggtcat gccgggatgg gcaacaccgc tcaggcgacc
     1861 ttctgcttct cgaagtagac gatcgccttc aagtatttga cggcctcgtt gaggaggata
     1921 tcctcgaatt cccctgattc cgtagcgtcc tggtcctcgc tggagaggtt gtcgcggcgg
     1981 tggcgcagga gctcgagcat ttgctgaccg tcgggggtct ccccctcgcc cgtcgccggg
     2041 gcatcggcgt cgggaagggc cggcgcgttc tcgtcgggag ttccctgctt tagttcgatc
     2101 atgtcgtggg tcgggccttc ccgggattcg tcgagcgagc cggtcgtgat ttcatcgtcg
     2161 agcgcggggg ttccctcgag gcccggctcg atctgcggcg gatcaccaag caggccatgg
     2221 cggaagagtt cgacttcgtg accttcgggc agctcgacgc cgaaatcggg cgcgattccc
     2281 ttttcatgaa tggacttgcc cgaaggggtg taatacagcg ccgtggtgag gcgcaggccg
     2341 gaaggtttgg gattgccgtt ctcatcgcgc tcgagggagt ggcgcagcgt ggagatggtc
     2401 tggaccgacc ccttgccgaa agtcgtggag tccttcggac cgatgatgat gccgcggcgg
     2461 gtgtcctgca ttgcgccggc gaagatctcc gaagcggagg ccgagccatg attgaccagg
     2521 acgaccagcc actggtcgca gagcttgtcg ttgagggcgt agtacttgcg attgttggac
     2581 tcgtggcggc cctggatcga gacgatcagt tgattcttat cgaggaagaa atcgcagatt
     2641 tcgacggcgc gatcgaggag gccgccggca ttgaagcgca gatcgacgat gacgccttcg
     2701 acgccctgct gattgaagtt ggtcagcgcc ttcttgacgt cctcggcggt gtcgtcctgg
     2761 aacttggtaa tgcgcaggta gccgatattt tcgtcgatga tcttggagaa gacgctctcg
     2821 accttgatca cctcgcggcg gatcgtgaag tcgagcaatt cgggtgcgcc ttcgcgccag
     2881 atcttgacct tcacctcggt gccggtcgga ccggtgagtt tgttgacggc ttccatgagg
     2941 gagatgtttt cagtcgactt gccttcgatt tcgacgatgc ggtcccaggg ctggacgccg
     3001 acgcgcgcgg ccggcgaacc ggcaatcggg gaaatcacgg tcaggatctt gttcttgtcg
     3061 agcgtgatgt gcaggccgac gccggagtat tcgccctcgg tatccttggt gagcatctcc
     3121 tgatcgacgg ggggcagata ggagctgtgc gggtcgagct ccataaacat gccattgatc
     3181 gcgccctcga agagtttttc cttatcgacc tcctcgacgt aattggcgcg gatctgggag
     3241 tagatttcgc tgaacagttc ggcgaactgg tagaagtcgg cgtcgtactg ctgctgttcc
     3301 tccatcgcgg cgatatgctg gggcatcttg accatcagca gcacgccgac gatgaccagc
     3361 atggtgcaga ggacgaggat gcgacgttcg agcgcgccgc ggcgcgggtg gaggggagat
     3421 tggcgtcgag tcacgtaggg gtctgtctcc gtcgggctga atcaggggtt gaccggccct
     3481 cgcgggccaa tcggggtatt ttagcaaacc gcgcggaaga ggcgcaatgg ggcgcatcgg
     3541 gcaagcattg ggaatcccgt ggatcggagc ggcccggcgg agggggcggc gcggcaccct
     3601 gaggggagag agggatccgg ggcgcggcta ctcgtggcgc agcgcatcga tcgggtccat
     3661 catcgccgcg cggcgggcgg ggtagaggcc gaagacgacg ccgacgatcg ccgagacggc
     3721 aaacgagaga atgaaagcga agggggtcag cacggtgggc atcgcggaga gcttggtgac
     3781 catcagcggg atgccggcgc cgagcgccat gccgatgagg ccgccgccaa gcgagaggat
     3841 gagacactcg acgaggaact gcgacatgat atcgcggcgg cgcgcaccga gcgcgcggcg
     3901 gacgccgatc tcgcgcgtcc gctcgctgac gctggcgagc atgatgttca tgatgccgat
     3961 gccgccgacc aggagcgaga tggcggcgat cgaaccgagc acgatcgaga agacgcgctg
     4021 ggtccggcgg gcctgctcga gcaggcgttg cggcacgacg agccggtaat ccttcttcgg
     4081 atggtgcttg ctcaggagtg catcgacggc gttggcgact tcgatgacgc gatccagttc
     4141 gtcgacctgg atgatcagtt cgctgagctc gaccttttcg gcgctggagg agccctggcc
     4201 ggtatggaag gtcgtgtcgc cctggcggag gcgttcggtg gtgatcggga cgaagcagtc
     4261 ggcggtctgg ctggcgagct cctcggcgcc ctcgaccggg gcgcccccct tattggtgtc
     4321 gacgatgccg acgacccggt aagtgtcgtt ggcgatcttg accgactggc ccagcggatc
     4381 ctcgaagagg aagaggtcct tcgcgacgcg gtcgctcagg acgatgtggt tgaagcggcg
     4441 gtcgaggtcg agctcggtca ggtagcggcc gcggacgacg cgcatcgggt ggttctcgat
     4501 gaaccagggg acggtcgcca ggaccttgac ctgcaccttg cggctgccga accacaagtc
     4561 ggcggggcgg gatttgatcg gcacgagggt ttggataccg gggatggttt ccgaaatccg
     4621 ctcggcatcg aggtaggtca ggccgtactc caggaccatg ctgcgcgagt tgttctcgga
     4681 ctgcgcgttg gggttggggg gcggcttgag gctctggacg aggacattct ggctgccgag
     4741 gctgcggatc tgggcctgga cgtcctggct ggcgccttcg ccgatggcga gcatgctgat
     4801 gaccgagcaa acgccgaaga cgatgcccag aacggtgagg gtggaacgaa gcttgtgggc
     4861 cctgacgttg gtgatgccga ggcgaataat gcgcagtgtc aggcgaagcg agttcagcat
     4921 ggcggcgggg atacaaagga cataggggac ataagtaatt caggctttgg aggccgaaca
     4981 aggggaccag ggaggctgga gcggaaatat cgcgtcagcc ttcgcccacc tttgccggcg
     5041 cggcgctgtc gtgggcgagc ggggagtccg cgggggcatc ggcggcgggc ttgccggcgt
     5101 cacgctccgc ctcggcggtc cgttcctggc ttctggccgg cttggcttca ccctgggcgt
     5161 cttcggggct gttggagggg cgttcgatca cgcgccggcc gcccccctga tccggggcgt
     5221 taagccggat cgtgtcgccc tcgttcagcc cggcctcgac gacgacgaag ctttcgttgt
     5281 tgcggccgat ctcgatcgga tgcttgacga agccgagatc cttgcgcagc cagacgaagg
     5341 cctgattgcc gctggtcgcg accgactgaa ccggcacgta gaggacgtcg tcgagttcgg
     5401 ccaggacgat ttcgctgcgg gcgctcatgc cgggcttgag cggcaacgtg gtggtcgtga
     5461 cggccagatc gacgttgtat tccttgacgt cgggcatgaa ccagcggccg ccgctgttgg
     5521 ggaggacgcc gatcttttcg accaacgcgc tcaggcgctg attgggcagg gcgtcgacca
     5581 tgacgatggc gggcaggccg acctcgacct ggcggatcat cgactcatgg acgcgggttt
     5641 cgacgatcca gctcgagaag tcgggcaatt cgatcagctt ctggcggtag cggacctggg
     5701 cgccttcctt gatttcgtcg tcgttgccgc gccaggaatt gccgtcgttg gcatagacga
     5761 ccatcccggc ttcgggggcg tagatcacgg tgttggccat ctggtgctca agggtctgca
     5821 ggcgggactg ttcgagctcg agtttggcct tggccgattc gacggcgatc tgcttggtgc
     5881 gcagttcgct tctgctactc aattggacgc gttcgagttc cccgcgggcg gcggcgacat
     5941 cctgctcgag cttgctcagt tcgcgttctt cctcgtattt gacgaggacg tcgtagtcgc
     6001 gcttggcctt ttccagttca tggcgctgtt tctggacggt cagttcgtcg gcctcgactt
     6061 cgccgcggct gacgaactcg ttttcctgga gcatcaaggt gccttcgagg cgggtttcgg
     6121 cgcgcttgag ttccgcttcg gccagggaga tggcggcttc cttttcggcc ttgagccgct
     6181 ggagctcgcc atcgcgatac tttttgaggt ccagctcggc cagttcgagc ttgttctgcg
     6241 cggcgagcag atcggactgg ttctggcttt cctggatcgc cagctgctcg acagcctcgt
     6301 cgtaggcggc ctgggcggtg gcggtgtcga tccgctgctt gacgaggtta tcgtcgaggc
     6361 cggcggcttc gagctcgacg agtttgtcgc ccttctcgac gatcgtcccc tccgggatga
     6421 tccagatgat cttggcctcg gcgttggaga tttcgcaggt gatggagcgg ctgttgcggg
     6481 ccttgatcga gccgggggcg aggagggaaa tcttgagcgg gccgcgcgtg accttgtagg
     6541 tcaattcgcc gccggcgtcc ttaccgacgg cgtcgtgctc gggcttggag aggccgcgca
     6601 tcgccagcag cgaaacgctg acgaccgcca gcaggccgac gacggagatc aggcgcaggg
     6661 tccagccgcc gcggcggcgc tcagccgttt gatagctgtt catgatccag gttcctcgtc
     6721 gttgtgtcgg gcgccatctc gtgccacgcg ccgcgctcgt cgatttccag ggcttcgatg
     6781 gcattgtaga actccagccg tgcgatcaga tagttaacca gctcgcgggt gagctcgttt
     6841 tcggcgttgc gcagggcgtc ctcggcttcg agccggtcgc ggatgctgac gccctcgcgg
     6901 cccagatcga ggagcatatc gacgctctgg acgcgcttgg cggccagctc gcggctggcg
     6961 agctggattt cgtaactttg ggccgcctgg accgcctgct gataggttcg ccggacttcg
     7021 gcgatgacct gattgcggag gcggtcggcc tggcgctcgc ggagggcccg ggtgatcacc
     7081 gcacggcggt agtcattgcg ttggaccttg cggtccaggg gcaactccag gtcgaggccg
     7141 gcgccgtatt ccctcaattc gttagtcagg tcaaaggggt gattctcgcc ggagtcgcgg
     7201 atgacgtaat cgaagcgggc gtcgaggacg ggcaagaggt tgttctcggc gaccttgagc
     7261 cggcgggcgg catcctcggc cagggcctgg gcggtcatca ggtccaggcg gcgtgcgacg
     7321 gccacctgct gcgcctcctc caagccatat tgtaactcaa cgaggccggc gttgcgaagg
     7381 gcatcgagct cgcgctcgtc ggggaggatg gtcaattcgg ggggcagacc gaggtcgacc
     7441 ttgaactggt cgaggaggat gccgtagtcg gtgcgggcgg tgagccagcg gttgcgggcg
     7501 cggatttcgt cctggcgggc ctggtcgacc tcgaacggcg ccatgcggtc ggcctcctcg
     7561 aacagttcgg cgcggcggcg caggacggtc aggcgctggt aggcccgcca ttcattgtcg
     7621 atctggtcgt aggcttgcag caaacggaaa taatcgtcaa tacggtcaat caggaaactt
     7681 ttttcgaagc gggagaaatc gcggacggcg tagatgacgt tgcgctcggc ctggcgcagg
     7741 ttttccagat tgacgtcggt cccggcgccc tggaggaggg gctggacgat ggaagccgag
     7801 agggcgccgg tatcgacgtg gcgcttatcg ccggtgtaga agcggaggaa attgttggtc
     7861 aggccgacgg tgaggcgggc gccggtggcg aacagtttgg tgacggcgag agaggtctgc
     7921 atctcgccga accgctcggc gatggtgcgc tcttcggtcc tcgtgcgcgt gacgacggtc
     7981 ggcatccccg tgacggggtc ggtcacttca acctgttcgg tgctcacgcg ggtttcggtg
     8041 gcgggaacgc ggttcatgcg cgcggaaatg agaccggtga agatgggcga gaactgatgg
     8101 cgctcctcgg tcagctccag cgcggtcagg tagaggtctt ccttggcggt ctggtagtcc
     8161 tcgttgttgt cgatggccag ggcgagcgta tcgctgaggg aaaggacgag gccggtggtg
     8221 gtgaagggat cggcgctgcg cccggcctcg gcgtaaatgc tgcgggtgcg gtcggattgg
     8281 tccggctcga tggtaaacgg ttggcgggcg ccgagcgcct cctcctgctt ctcgccgatg
     8341 atctggtagg tttcgcgatc ggcgagacgg tgggccagat tgccgaagca accggtcagg
     8401 aggaggccga ggccgcagat cgagagcgag ccggcgatgc ggatgaggct ggcgacagag
     8461 ggaaattggc tgaatagcat cacaagcgtt gccggaaacc ggcgttcaag ttcgagccaa
     8521 ggtccgccgc cgtggcgctg atggccgctg ggggcgagcg gggcgtgctt caacaagagt
     8581 caagaccccg ccaagcaaaa ggaagtttgg agcggcggcc agcgtggatc ggccggcggc
     8641 gcgaattggg ccgacgcggg ggcgtcagcc ggagcccagg ttgatctcga ccgggcggcg
     8701 ccctccttcc agattctgga ccgagacggc ggccgccagt tccccggcaa tccgccgaaa
     8761 ttgcaggctt tgctcgcagt cgggatcggc cagcaggacg gggacgccgc tatcgccccc
     8821 ctcgcggaca ttctggccga gcgggatgct gcccaggaaa tcgatgccga gctccctggc
     8881 cgtgcgctcg ccgcctccct cgccgaagag ccggtggatc ttgtcgcagc cgtcgcagcg
     8941 gaaccaggcc atgttttcga tgatgccgag gacgggggtg ttggtctgct ggaacatggc
     9001 caggccgcgg cgggcgtcct gggtggaaac gagctggggg gtggtgacga tgacgcaacc
     9061 ggtgacctgg acgagctgaa cgagggagat ctggacgtcg ccggtgccgg gggggagatc
     9121 gacgaggagg taatcgagct cgccccagtc gacatcgccg aagaactggc gcagcgcgct
     9181 gttgagcatc ggtccgcgcc agacgacggg ggagtcgtcg gggacgagca ggcccatcga
     9241 gatcatcttg aggccgtgct tttccagggg gacgatctga ttcttgacga cgcggggggg
     9301 ctcgcgcaga cccatcatcg tggggatgtt ggggccgtag atgtcggcgt cgagcaggcc
     9361 gacgcgggcg ccggactggc tgagcgcgac ggccaggttg agggcgacgg tcgatttgcc
     9421 gacgcccccc ttgcccgagg cgatgccgac gatgttgcgg acgccggggg cgaggtcctg
     9481 ctggccgcgg gcgcggccgg cgacgtcggc ggtgaattcg atctcgacgc cgcggaagcc
     9541 ggggaggggc tccagagccg cgcggacatc gcgctcgatg acgctcttga gggggcaggc
     9601 cggggtggtg agcgccaggc ggagacggat cgcgccgtcg gcgatgtcga tccgccggac
     9661 catcccgagc gagacgagat cgcgattgag ttcggggtcg ttgacccgcg caagcgcctc
     9721 gcgcacggct tgttctgtca gactcatggg ggggtttctc cggcaagcgg gccgaggtcg
     9781 gaggcgacgc gtggggatga gggggaggtc cggacatcgg acgcgtcgct ccatattgta
     9841 gcgtgaagtg gggggtggag gcaatggacc aatggacgca gtggacgggg tggacggagg
     9901 gggagaccgg gatattcggc ttgatccggg gggccgcatt tgacaagttc gcggagatgc
     9961 caagtttgcg gggctgccat tcaggcttgg aggaggattg ccatgcgcgt gtcgttgcca
    10021 agggccttcg tcaggctgag cgtctgggcg gggctgatgc tgctgccggt cggcgcggcg
    10081 ggcgaaacgc tgtcctacgc cgacctggtc catcgcctgg tggatctgga acggctggcg
    10141 ctgctgccgg cggaggggga agtgtgccgt caggcgtcga gctacgaccg ggccagcgtt
    10201 tacgacgaat cgacgggtcg ctatgtgaac tggagcgcga acaatgacgg cgacgggttc
    10261 atccggcagg tcaacggccg gcaggtgctg gccgagatcg agggccccgg gtgcatctgg
    10321 cgcatctggt cggcggcggc gcggggcggg cgcgtgcgga tctatatcga cgcgcaggac
    10381 aattttgaat cggccaccgt caatctgccg ttcaacgggt atttcaatcg cgagaacgag
    10441 ccgtttacgt atccggggct ggtctatcag acaccgagcg gctacggcta cgaggggggt
    10501 tacaacagct acgttccgat cccgttcaac cagtcgtgcc ggatcgtggc cgatccgggt
    10561 tgggggttgt attaccattt cacctacagt cagtttccgg aagggacgac agttccgtcg
    10621 ttcaagcggg atctgccgga ggaaggccag gccgcgctgc gggaagtgag cgattttttc
    10681 gagcggcgtc tgggcgagga tctgggcggg ccgcgcgaaa acgcggagac gacggtccgc
    10741 gcgatcgagg cggccggcgg gggggcggcg acgcgcgtgg ccgagctgag cggtccggcc
    10801 gcaatcacgg cgttgaaagt gcgaatgccg gagatggacc ggaagacgca gcagcgggtt
    10861 ctgcgcgagc tcgtgctccg gatcacgtgg gacggggccg aagcgccggc ggtgtggtgt
    10921 ccgctggggg attttttcgg cacggcgccg gggatgaacg aattccgggc gctgcccgtg
    10981 ggcctgatcg gccagggatt ctattgttat tggtatatgc cctttgcgac gtcggcgcgg
    11041 attgagattg tgaatgacgg ggccgagcgg cgcgatctta tgtttgaaat catcacggcg
    11101 ccgctggagc ggccgatcga atcgctggga cggtttcacg ccaagtggca ccgcgacgca
    11161 ttcatcccgg aggcggaaac cggccgcgcg atcgactggc cgctggtgaa gacgaccggc
    11221 cgcggccggt tcgtgggcaa tctgctgaat gtgtggaacc cgcgcggaag ctggtggggg
    11281 gaaggcgacg agaagttctt cgtggacggg gagaaattcc cctcgacata cggcaccggc
    11341 agcgaggact actacggcta cgcgtggggg ctgccgctgc tgtttgcgaa ggccttccac
    11401 agccagaccg tggcacagcc ggagaaccgg tatcacattt cggtcaaccg ctggcacatt
    11461 gcggataatg ttccgttcca gaccgagctg gaggcgtgca tcgagaagta ttatccgaac
    11521 agccggccga cgatgtattc ggcggtcggc tactggtatc tggatccggc ggggaccgat
    11581 ccgtacggac ccgtgcccgc cgaagagcgg gtcgattatt acgtcatgcc cgagatcttc
    11641 cggatcgagg gggtcatcga ggcggaggat ctgccgacga gcacgggggc gggcttcgcg
    11701 cggttccagg atctgacgct gtgggggcag aacgcgtgga gcggcgacgc gcacctgtgg
    11761 tggaccgagg cgcgcacggg cgaccggttg agcatcacgt ttccggtcga caccgccggc
    11821 gaataccagc tgatcgcgtc gttcacgaag gcgccggact atgggacggt gcaggcgtat
    11881 ctggacgggc agccggtggg cgaaccgatc gatctgtaca tctcggtgtg ggtcgagccg
    11941 acgggcccgg tcgatctggg gcggcaccgg ctgacggcgg gcgagcacac gctgacgctg
    12001 cagatcacgg ggaagaacga gaacgccgcc aatacctacg tcgggctgga ttacctcaag
    12061 ctggtcgcaa cggggacggg ggcgggggac gattgagttg gccggcggct actcatagac
    12121 cgcccaggcc ggcccgccga cggcgttgcc gcgttcgacg gcccaccaga tcaggtcgta
    12181 gttgaatgtg tcgagtcccg tgacgggggc gacttcgagg atgtaatcgc ccgggccatc
    12241 cacgaggtgg ccgaggacga gcgtgtcggc ggagctctgg tgctgcgcca cgagggcgcc
    12301 ctcggggtcg aacaggcgca ggaccgcatc gatgttttcc aggtcatggc gcaggtggac
    12361 gacgacacgc tcgcgccccg ccgggaccgg caacaggtaa taatcgcgat cgagccaacg
    12421 ggcgccgccg tcgaaggtcg agagccactg cccctcggcc tgcgcgaggt tcgccgcgct
    12481 gttgggcgag tcattctgtt cgtagttgtc gtcgcccgag ggatgcggcg gcatgccgtc
    12541 ccaccacagg ttgtagcggg cggtcgcgac gttggccccg tagaggcgca gtttgtattc
    12601 cgtattgggg gagatgggca ggtcgattcc ctcgttgtcg gtcagcgaaa tctgctggcc
    12661 gaggagcgtc tggccggtcg agtcgtaaac ctccaggtcg atgttgccga ggtcgtgttt
    12721 gaacaggacc tcgacgacga ggcgctggtc gttgggaccg gtctcgacgg tgaaccagtc
    12781 gtcgtcggtc tggtagccga agccgttgat caacgagagg taggttcccg cgaggaactt
    12841 gaggttcgtc gcctgttgcg gggtgtcgtt ttcctcatag aggtcgtcgc cgggggcgag
    12901 ggtgtaatgg cgcaggtcgt agacctggcc gctgttgttg ccgacgacgc gcagccagta
    12961 ttcgccgggc tggggcagct cgaaatcgag cagatcatag ttgctgtttg cgcgcgaagc
    13021 gtcgaccaga ttgccgccga tgccgtagag ctcgaggtcg aggttgccgt cggcggcgat
    13081 ggagatgagt tgcgcgaaga gatgggtcaa gccgtcaggc atctggatgc ggaaccactc
    13141 gtcgtcgagc tggatcccga ggccgccgat ctgggtcagg tattcgccgg cgtggggagc
    13201 cagattgaac gcgccgaggc gggtgtcatt ctgttcgaag gtatcgtcgc cggttggacc
    13261 gaagcggtag acgaaatcgt agggattgcc catgtactgg ccgtagaatt ggatcgtata
    13321 gcgtccgggt ttgggcaggg tgagccgtgc cgtttcctcg ttgctctggt tcgttccctc
    13381 gtcgatcagc atctggtcgg ggtcatagag ccgccaatta acgtcgccgg cgaagaaatc
    13441 ataggtcagc cggaggaaga gggtgagcat gccctcgggg acgtcgaagg tgaagtaatc
    13501 cagatcgccc aggcggccgg ggcccatgat gcggctcaag cgttgaccca tgaaggggga
    13561 caagttgcag gcggccgcgg cggagtcgtt attctcgtag gcgtcgtcgt tgccgctctc
    13621 gcggttccag aggagattgt aggtgccgcc gcccggcccg aagtgaaaga cgcgaacgaa
    13681 ataattgccc tggttcggca gttggacatg gaggctttcg aagtcggttc tggaaaccga
    13741 ttcggccagc ggcgttccca tggaatcgaa gagctgcaga tcgatgtcct gctggtcgtg
    13801 atcgaacaga cagacgacgt ggagccactg atcgggggcg ttggtgctga cggtgtagaa
    13861 gtcgacgtcg cggatgatgc ccgggccctt cagatcggtc agccactggc ccggggtggt
    13921 gacgagggtg cgtgagaagg gctgggtgtc gttgtcctcg tagaaatcat cgcccgcttc
    13981 ggcggtcgcg ccccaccaga gatcgtaggt attgcccctg tcctcgccga agacgcggac
    14041 aacatacaga ccgggatcgc tgatcagcac gtcgaggatt tcgaaatcgg cggttccatc
    14101 gacgacggcc agcggcaggc cctggggatc gaagagttgg aggcccatgt tgcccgcggc
    14161 gtgcttgaac cgcagggtga cgaggaggcg caggggggcg ttgctcaggt ggattgcgaa
    14221 ggtgtcctcg tcggcctgga tgccctggcc gcgcagcgtc gagaggtgca acgtgccatg
    14281 gggggtgatg tcgaaggcct cggagacgcc attattgtgt tcgtaggcgt cgtcggcggc
    14341 gggataacga ttccaataga gattgtaatc ctgggcgccg ctgtaggaga cggcgatgac
    14401 gcggatgtag taggagccgg cggccggcac gacgaagttg atcagctcgc ggtcgccggt
    14461 accgagcgac tgcgcgagga tgttgccggc gccgtcgagg agttggagat cgaggtcgcc
    14521 ctcgctgtgg tggaagcgcg catcgatgac gacgcgcgtc gcatcgtcgg tggggttgat
    14581 gcggtaatag tcgacatcgc gcagaatgcc ggggccatcc aggttggtca accagtggcc
    14641 ggcgaagccg gtggcgttga cggccgtggc gatcgagtta ttgggtgatt gttcatactg
    14701 gtcgtcgcca gcgagcggga gggcattcca ccagagatcg tactgggtgc ccagatcggg
    14761 tccgctgacg cggaggtaat aggtgcccgg aaagaggaca ttgaaatcaa tcagctcata
    14821 atcctcgagc gagcggcttg tcgtcagcca gtcctcgccg gcggcgggga acaggtcgag
    14881 cgacaggttg ccttccttgt tcttgaacgc gatgctggcc tggacgcgac catttcccgg
    14941 ggcaaccgta atggcgaacc aatcctcgtc attctgatag ccgtaactgg cattccagga
    15001 gctgaggcgc acgccggcgg cggcggagat gtcggccgcg gtctcgcggg tgtcgttctc
    15061 ctcgtagaaa tcctcggccg cgtgggcgag gccggcggcg cagaggagca ggcagcccga
    15121 gatcaatccc gccgccagcg ggcgggcgga tgcgtgacgc aggttcatac cgcgaatctc
    15181 ccgactatca gatagggctc gtccggaggc gaacggcgcg gcgcccctcc gggccgcgtc
    15241 gatcggcacg agaccgacta tggagttgga aatcgtgtcg ccggcaggcg tgccgcgaat
    15301 gcacctcaaa ggaccagatc cggtccgggt cccggcgggg cggcgaggac gccgcaagcc
    15361 ggagcgagac ggagtcggcg ggcgggttgg actccgtcgc gggcgccggg gaacatcctt
    15421 cattgcgctg tgcggaccgc gaagaatata tgaccggagc gcgttacgcg gcaagtggaa
    15481 aaattaactc ggaaatcacc gtccgatatc ggaaatcgca gaggaggagg gggaagagga
    15541 aggccatggg aaattgaacg caaagggacg aggactcgat gaagaaggcg tttgcacggg
    15601 gggggaattt gagcaaatgg cgccagaacg attgcgtgcg cgggcgcatt gatatatgac
    15661 agagcttcgc gattggcgga cgaactggag aagaacccgc gggtgtcgaa cgaggggtaa
    15721 aggggcgtag gtcgttggcg gcgaggacga ggacgagaga cgagggacga tttgaggagc
    15781 ggcatgggcg ggttcattct taatttcacg ccgaacggga tggtgccgac gcgcgagatg
    15841 acgccgcacg tgccgctgac gcccgaagag atcgtgcgcg acgtcgacga ctgcgtgcgg
    15901 ctcggggcga acatggtcca cctgcacgcg cgcgacgcgg aggggcgccc gacctacgac
    15961 aaggagatct atgcgcggat catcgggggg gtgcgcgagc ggcatccgga gctgctgatc
    16021 tgcgtgtcga cgagcgggcg gctgcacaat gaattctggc agcgggcgca ggtgctggag
    16081 ctggagggtg acgtaaagcc ggatttcgcc agcctgacgt tgagctcgct gaatttcgcg
    16141 cgcgcggcca gcatcaatga gccggcgatg atccaggatc tcgcgcggcg gatgctggcg
    16201 cgcggcatcc ggcccgaact cgagtgcttc gacctgggga tggtcaacgt cgcgcagtat
    16261 ctgatcgaca agcaactcgt gccggagccg tactacgtca acctgatcgt cggcaatctg
    16321 gcgggggcgc aggcggacct gatttcggtg ggggcgctgc gggcggcgct gccggcgggg
    16381 gcttactggt cggtcggcgg gatcggccac agccagctcg tcgccaatac gctggggctg
    16441 gccgccgggg gcggggtgcg cgtcggtctg gaggacaacc tgtggctcaa tgaagggcgg
    16501 acgacgctgg cgagcaacgc cgcgctggtc gaacgcgtcg tgcggatcgg cgggctgctg
    16561 gggcgcgagc cgtacagccc ggccgaggtt cgagcgctgc tgacgaatcc ttgaactgat
    16621 tccgttcaat ttcattggat ttatcgccgg acacaaattc caatgacaaa ttgaaaacca
    16681 tggctcaggc cgatcccagc ccccgttcca cgccggcgcc gggcggcgcg tccgtctggc
    16741 gcaagctgcg cgatgtgatc cgccatggcc tggtgattca gacgctgctg gacgtgctgc
    16801 gcaaggcggg gcttgagatc acaccgtatt acgtcatgcg cgaggcggtc gtttcgccgg
    16861 acgcggcgcc ggtcgccggg ctggagaacc tgaggctggc gtggctgggg ccggaggacg
    16921 cggcgcaggt cggcgcgctg cccgacagca aattcagcga ggccgagacg cgggcgcggc
    16981 tggcgcgcgg gcagcgctgc ctgggggcgt gggtcgggtc ggagctggcg gcgttcatgt
    17041 gggtcgatac gggggagctg gattttgagc cgtgccgagg gccgctgcat ccgcatgagg
    17101 cctacctgtt cggcgcgacg acgacgcgcg cgcggcgcgg ccacaacctc gtgccgcatc
    17161 tgcgcgcgga gctgtatcgc gcgctgcggg ccgaggggcg aacggtctgt tacagcattt
    17221 cgtatgcgtt caacacgccg gcgctgcggt tcaaacgcaa gctgaacgcg cggtgcgacc
    17281 ggttgtatct gtacgtctcg ctgtggggtc tggcgcggcg caactggttg ctgcgacgct
    17341 atcccgccgc gccgggcgac ggcgagccgt gagcctaccg aagcaaagca ccctgggccg
    17401 cgcattgacg gcggatttct gggccgccgg atttcgcccg tttatcattg gaattttcgt
    17461 gcttgggccg ctgatgctgc tggtcaccag ttacaccggc gacagcgacc agttgatcgt
    17521 gggcgacggg cgggagtatt tcgaatacgc gcgcgtgctg gtgattgaag ggcacctgcc
    17581 gtacgagcat ctgcgttatc cctgcggggt gtcgctgatc gggattatcg ggtatgcgcc
    17641 gtcggtctgg ctggcgcggg cggccggctg gctggagcgc gggcgcgcgg ccgagggctg
    17701 ggcgattttc aatcagttca gcttctgcgc gccgctgatc ctgctggcgg cggccggggt
    17761 gtgggccaat ctgcggatgc tggcgcggct gggatttgcc ggtccggtcg cccggttcag
    17821 tctgatgtgc tggctggtgg ggacgaatct gccgtggtat ctgttcaagg aaccggcgat
    17881 gagcgagagc gccacgttca gcacgctgtc gatttattat tatttattga tccgggacgg
    17941 atatggaaac gtttccgcgc ggccggaccc ccggcgctgg gcagtcattg gattttttct
    18001 ggggctggcc ggggcgattc gccagcagaa catcctgcac tgcgcggccg tgccgctgct
    18061 ggtcgtgctg caacaacggg gggtgtggcg cgagcagccg cgccaggcgg tgcgaaatgt
    18121 ggtctggtgc gtcgcgccgg ccgccgcggc gagcgcggtg ccgttcgtcg ttccgtggat
    18181 cgtgtggtcg ttcgcgcgcg agggggcgcg gctgtatgcc tatggttcgg agggatttga
    18241 ttttctgcac ccgcggctgc tggagatcct gatcctgccc ggctatcacg ggtttttcgc
    18301 gtggacgccg atgttcgcgc tggcggcgct cggctggata ttcttttttc gccggcaccg
    18361 cgggctgttc gcgccgtttc tcgttccggc gttcctgcag tactacctga ttgcgtcgtg
    18421 gtgggcgccg agcttcgggg cgagcatcgg ccaccgcggg ttcatcacga ttctgccggt
    18481 gctgctgccg ggtctggcgg cgctgctgga gtggagccgg gcacgcggcg gacgcgtgga
    18541 gcgggcgctg gcggcgctgc tgctggcgct gatcgcggcg aatggcgtgg tgctgctgat
    18601 gctggccacg ggacggctgt cggcgaactg aacgcggcac gggccatgag cacggggcga
    18661 gggttattcc cgcgccgcca tttcgatcca gacgcggatc cgggccgtat tcatcccgag
    18721 catgaccgtg gcgaggagca tcaggccgta gcccatcatc tggctcatcc cgacataggg
    18781 gccgacgagg ccgaacatgg cgatcgactc gaagagggcg tcgctaagga tcgccatcag
    18841 cttgacgcgc gcgaggccgc gcgaagcggt caggttccag aggagcgcgg ggagcatgaa
    18901 gccggcgggg atctgcgcga cggcgacgac gatcaggagc agcgcggcgg tgcgctgggc
    18961 cggcgccgcg gcggcgtcgg gggtggagat ttgcggaaac atgacgaacc aggcgaggag
    19021 gaggaaaaac ggaaccgagg cggtcatcgc ccagtgaacg agccagatgg ccgactcgcc
    19081 ggcgttgccg gccggacggt tattgacggg ggtcatccgt ccgcttcctt gcgccagatc
    19141 ttgcgcgggt cgtcggggcg cagttcggcg tatttggcgt ggatcatctg gaaatcctcc
    19201 cacgcgcgcg gcttgaagcc gggtgaacgc aggaggaacg cggggtgata ggtggccagc
    19261 agcgggatgc cgtgatagct gtgccagtgg ccgcgaagac ggccgatggg gaggtcgctg
    19321 cggcagaggt actgggcggc gtgggcgccg agcgcgacga ggattttggg ctggaccagc
    19381 gcgagctgct cgacgaggaa atgctcgcag gcggcctttt cgttgggcag cgggtcgcga
    19441 ttgtccggcg ggcggcactt gagcgtgttg cagatgtaga cttcctcgcg ctggaagccg
    19501 atggcggcga gcatcttgtt gaggagttgg ccggcgcggc cgacgaaggg gatgccctgg
    19561 gcgtcctcgt cgccgccggg ggcctcgccg acgaacatga tgctggcttc ggggctgccg
    19621 tcgccgtaga cgaggcgttt gcgcgtggcg ccgagcgcgc aggcgcggca ggcttcggcg
    19681 cgggcgatgg cctcgcgcat gcggtcgagg cgctgttcgc gggggagggg ggggccgaag
    19741 gcgggatcga gcgtcggtcg cgagggggtc gccgtcgggg cgaacagatc cggcggcgcg
    19801 gagggaatgg gctgggccga tctggcgggg cgcgcggggc gcgcggcgct cgccatcggg
    19861 cccggctgga gcgaaccgcc ggacggggcc gggggagagg acttgcgcgc ggggccgggg
    19921 ttcgtcgcga aggtcagcgc cggcagcggg ccttccaggt agaggcagcc gctttccttg
    19981 aggaactgca ggtagttgag cagatggtgt ttgattgacg gcaccgtggg gctcccgcgc
    20041 tgattcgtcc cgaccttagc agaccgggaa aaaacgcgcc aagcgaaagg cggggccgcc
    20101 gccggtccgc tcgtgcaaga gcgcggatca gtcgcgctgg atttcgacgg tgacgctcgg
    20161 gctttcgggt tcggcagccg gcgcgcgggg ggcgggggcg agtcgcggcg ccggcggggt
    20221 cgcggcgggc tggtcggtgg gcagttggcg gttgacgcgc tggagttcct cgcggcggcg
    20281 gcgatcgcgg atcgattgcc agagggtttg ttcaagctgg tcggtcaggt cgatggcgta
    20341 ggcgcggccg ccgcgcagtt cgagggtcag ggggggccat tcgacgccgt tcggtccggc
    20401 gggcgcgccg gcgaacgtgc gcaggcgcca gcggccggcg gccagcgcca gccactgatc
    20461 gcccttgacg ggctggggca ggcggacggt ccggtccgtc gcgtccgcgg ccgagtcgac
    20521 gtcgacgggt tcgaggatcc aaagcgggcg gagccggtcc atgtcgttgc ggagccgcag
    20581 ggcggcggcg ccaggcgggg cctggacggc ctgcgcgccg gcgagctcgc gcagctccag
    20641 cgcttcggcc acgcggccgg cctggtcgcg cagaaggctg cgggcgcgca gctcggcgcc
    20701 tttttcctcg agccattcgg cggacggggc gcgaggggcg gcgggctgcg cgggggcggg
    20761 gagcggcgcc gcgagggcgc agagaagcgc aagggcggcg ggcagcgcga gggcgttcat
    20821 gatgcgccgg cgcccggatc cggcgcgcag acggcgcagc cggcgtcgcc gttgccgcgc
    20881 cactcgcgga cgcggaagcg gacctggagt tcgtcctcgg ccaggcggcg gcaggcttcg
    20941 acatcgacct cgggaacggc caccagattg agcgtaacgt cggggacgac cgcgcgggcc
    21001 gatttgacga agtcgagcat cgcgtcccag gagcgggcgg ggtcgcgcgg gtgggcaatg
    21061 cgattgtagg tctcgcggtc gtgggcgatg aggttgacgc cgacgctatc gaacaggccg
    21121 gccaattcgg gggtcaggtc gcgctgattg atcaggctgc cgttgccgtt ggtgttgagg
    21181 ccgacgcggg cgccgcgctc cttgagaccg cgggcgacct ggcggcagac gtcccagcgc
    21241 atcgtcggtt cacccaggcc gctgagaaca atttcatcat actggtggat gtcggcaacg
    21301 cggtcgagca gttcagcggc cgttggttcc tcatccagtt tcagaaaatg gcccatgacc
    21361 atgtaatcct ggcagcgctg gcagtagtag cattcgttgg agcagcggtt ggtgatggag
    21421 agatagaggt tgcggcggat agcgtaggtg acggcgccgc gggcggcgtc ggggaggtcg
    21481 aagaggcgga ttgcgttggt tttggtgatg cgggcggcgt cctggtagct gacgccggtc
    21541 aggtcggcga gcatcttgac cgtgtgtttc atgtaggagg gttcattgcg gcggccgcgc
    21601 ttgctctggg gggggaggta gggggcgtcg gtttcgcaga ggacgcggtc gaggccgatg
    21661 cggctgacga cgtcgcgcag ggcggcgccg ttggggtagg tgatcgcgcc gccgaggccg
    21721 agatggaagc cgaggcggat gagttcctcg gcctggggca gatcgccgga gaagcaatgg
    21781 acgacgccgc ggcgggggat gcgctcgtcg gtccggatca ggtcgatgag gtcgtcgtag
    21841 gcgtcgcggc aatggatgat cagcggcagg ttgcggcggc cggcgaggtg ggcctgggcg
    21901 gcgaagcatt cgatctggcg ttcgcgttga taaccgggat agtggaaatc caagccgatt
    21961 tcgccgatgg cgacgacggc ggaatcgcgg gcgagccgtt cgagttcgag cagcgcgagg
    22021 gggctgaagg cggcctcgtg gtgggggtga atgccgacgg cggcggcgag gcacggctcg
    22081 tggcgggcaa gctggatggc gcggcggctg gattcgagct tgtcgccgac gacaatcagg
    22141 cgctcgatgc cggcatcggc ggcgcgggcc agcacgcggt tcagatcgcg ggcgaacttc
    22201 ttatcctgaa ggtgggcatg cgaatcgatg agcatcggcg tggaaccgga tggaaggggg
    22261 tccccaggga aatccgcctc aggcatgccc attttacccg tggcgacggg ggatgaaaac
    22321 ttgaaaaaaa gcggggcggg ctcagaactc tctgccggaa tcgccgggtt tcgcgagccg
    22381 aggacgaggg acgggtgccg agagccgaat tttggggggg cggggggaac agaggggaaa
    22441 aatgcgtgtc gggtggagca gaggagttgc gtcccatcct ttgatcctgc catgcggagg
    22501 tgatggctca tgaacgcgca tttgcaaatc tcgcggttcg ttgggctggc cggcttgatg
    22561 ttgctggcgg cgacggccgg cgcgcgccag gttgccctgg aggtgcaggc cgggcagccg
    22621 ctgctcaagg ccggcgaaaa gcagacggcc tacctgatga tcacgctgac ggggccggcg
    22681 gtggagagcg cgtcggaacg gccgccggcg aacgtggcga tcgtgctgga caaatcgggg
    22741 tcgatgggcg gggagaagat cgccgaggcg cgcgaggcgg cgctgctggc gctggagcgg
    22801 ctggacaagg aagatatcgt cagcatcgtc gcctatgaca ccgatgtgcg ggtggtgctg
    22861 ccggcgacgc ggctgcgcga ccgcgagcag atcgagaccg cgattcgcgg gatcgaggcc
    22921 ggggggaaca cggcgctgta cggcggggtg agccgcgggg ccgaggaact gcgcaagttc
    22981 caggagcgcg accaggtcaa ccggctgatt ctgctgtcgg acggtctggc taatgtaggg
    23041 ccggcgacgc cggaggagct ggcggcgctg gggcgcgaac tggggggcga ggggctgccg
    23101 gtgacgacga tcggcctggg gctgggctat aacgaggatc tgatgtaccg gctggcggaa
    23161 cagagcgacg gcaatcacgt cttcgccgag cgggcgagcg acctggcgtc gctgttcgat
    23221 gaagagttcg gctcgattct gcaggtggtc gcgcgcgacc tgcgggtggc cgtgcgatgc
    23281 ggcgaggggg tgcggccggt gcgcgtgctg ggtcgggatg ccgagatcga cgggcgcgac
    23341 gtgatgctgg agctggcgca actgcatggt gcacgcgaca cggacatcat cctggaggtg
    23401 gaggtgccgg cgcgcgaagc gggcgacagc ctgaagctgg ccgaggtgga ggtggagtac
    23461 cgcaacctgc tgaatgaaac cgacgagcga ctgacgagcg aggtttcggc gcggttcacc
    23521 gattcgagcg aggcgatcga gaaggcggag aatgacgagg tgatggcgcg ggtggtgcag
    23581 cggatcgcgg ccgaacgcaa tgcgctggcg acgaagctgc gcgatcaggg ggagatcgac
    23641 aaggcgcgcg agatcctgat cacgaacgag gcgtatctga aggaaggcgc ggcgcgctat
    23701 gatgcgccgg ggttgggggc gtatgccgag atccaggccg gtgacgcggc gaatctggag
    23761 ggggagaact gggagcgcca gcgcaagtcg atgcggtcga cgcagtatca gatgttccgg
    23821 tgaggcggac atcgggttgg gtgggttgcg ggggaaaccg ttgaaacggt tgggagaaag
    23881 ttgcgctgcc gggtgaccgg gccgaggccc ggcgccaata acaaggccac cgagggggcc
    23941 ctggaaaagc ctgctgacgc gggctctcaa ggcggcggcg aaagtccccg attccttcac
    24001 tggtcgaaaa gcggggagca ggccggcggc aaaccgtgag cattgcatga atcgggccgc
    24061 caagcagcca agccggtttc agtttgccgg aatccgcccc agacccattg agaacacctc
    24121 tcgcctcaga atcggccgcg gttgattgga tccaaagcgg tgtcgacgcg ttgcgatgca
    24181 acgctctgcc accgcactcg aagaagtcgg ttcgacgttg ctccctatgg cgtgcggcgg
    24241 cagagccagg cgcggcggca gtagatgtgc cattcgagcg tgaggaggcc gagggcggcg
    24301 aaggcgaacc agggccagac ctcgcggttt tcgcggctga gggacgagac cgcgatccgg
    24361 cgctcgccgg tgacgaggct gtctttgggg gcgatgtcgc tttcggcgag cgtcgtcacc
    24421 cggtcgggga gcgtcaggtc gagggcggcg ggacggggac gcggggattg cgcgcgagtc
    24481 gccaggcgtg cgatggggtc atgtagcgaa tcccgtccgt ggctttcatg ccgatctcag
    24541 tagagttttc gggtggtggt ggtcaggccc tcgcggagga gttcgttcgt gctgtagccg
    24601 gccgtgcgga tatggttttc gttgtagccg aggttgagca ggtcgcggac ggaaaagccg
    24661 gccatgcgca tttcgtcggc gcggaagccg gcctgcttca gttccgagcc gctgaagaag
    24721 gtgcgcaggt cgcgcgcgcc gaaattggcg cggcgcaatt cggcggcggt gcagacagtg
    24781 gccagcgcct cgatcgggaa gttgagctgg gccagttcca tggccgacag gccgaggcgt
    24841 ttgcagtcat ggatcgtgta gccctggctg tggagttcgc tggcgcgcag accggcggcg
    24901 atgaggcgcc ggagctgctc ggggccgcgg cccgcggggg cgtcgtgcgg cgcggccggg
    24961 gagaacccgg cggcgggaga gcggccgccg acggtcgccg gcgcgggggc ggcgctttgc
    25021 acggcgtagc ccagttgggc gagcgtgcgg cggtcgtagc cgagccgttc catgccttcg
    25081 gccgtgtaac caaacagttc gagatgcctg gcgccgaggc ccaggttgag cagccagtgg
    25141 gcgccgcggg acttgccctc gaccaccatc cgatgcacat gatccagccc ggagatgccg
    25201 agctggcggc acaatttgag gcgttcttcc ggcgtgagag gtttccgagt catcatccac
    25261 ctcgcaagat cgaaagtgac ggcgccaatt gatgcctggc gattggggag catctggtgg
    25321 cgcggctgga cccttaacta ataagtcaaa aaccgtcctg ggacaagggg attgtagggg
    25381 aaagggagag gggtgggggg tgaggcgaaa ggtagcaggc ttttcgatcc aaagcggcgt
    25441 cgccgaccgc tacgcggtct ctgccgccgc actccacaag gaacgtcagg atccaagggc
    25501 cggggggaag cgcgatggtt tcaggtgatc atcgggtcat gtcttgtcaa gatagtttcc
    25561 ccagtcccag tcgaaatcgt tcctgagcgg ttcctggcgg cggagggggc tgttttcgcg
    25621 gcagaagacc atatcggttt gggccagggc gtggtcgcgc cggcgccggt agaacccgac
    25681 gatgtcgaat acgacatagc cggccgcgtg cataaacgcg acgatatcgt ggaggatcgg
    25741 caggcgcggc cccatcagct tgagcaggga gacttccatg aagataactt cggtgatgcc
    25801 cagcgcctgt cgcgccccct tgaggacttc cagctcgaag ccctgcacgt ccagcttcat
    25861 catgtcggga atgcccacct gaccggcggc aatcatgccg tcgatcgtga tgatctacac
    25921 cttgcgcttt tcgtgcgact cgtagcccgg ctggtcgggt tgggggatga aggaggagcc
    25981 cgacagcgta ggccagatcg tgaatgtcag ttcaccgggt tcggctcccg cgccgttcag
    26041 atagtatcgg gcattgccca gcttgcgaca ggtctgctcc aggtgcggtt ccatctcgac
    26101 cagcggctcg atcatcacca cctcggtctg ggggaagacc cgccggaacg tgcccgacca
    26161 gtagcccttg tgggcgctga catcgagcag cagccggggc gaaaacccct tggcatcgag
    26221 aaagcgaaga aactgagtgg tctacatggc gttgtgccat gcggggtggt ccgtaaccat
    26281 ggattgactg cctatggaaa ggtctgcgcg cggccgcttt ctcgtgcaga tatactctct
    26341 ggtttctttg ctgtcgaccg tttcttagcc gtggggagat cagaggttga cccacgcgca
    26401 cgatgccgga cccgccgttc gcggtttgag ttgactgtgc cccgggcaca atcaaagatg
    26461 cgctgttcca aggccggaag cggatgttcg attcgcttcc cctgaactct gtcgaggtct
    26521 gtaagcgatg gcaaaacaac caacatccct gggagcgttt aacggactgg gcctgcacct
    26581 gggcaatcta tcgcgggtgt cgcgcgcgcg gacgcgctcg atcagcccgg agaatttcag
    26641 cggcgaaaaa gggaaagggg gcatggcgac cgaagggacg ggggcggagt gcgcccgcga
    26701 cctggggcgg ggctggaaga tttcgccgtc ggtgcggatc gaaccgggga cgacgttcac
    26761 gatggccgac atcgccggcg ccggcgcgat ccagcagatc tggctgacgc cgacgggcaa
    26821 ctggcggttc agcatcctgc gcatctactg ggacgacgcg gagcagccgt cggtcgaatg
    26881 cccgatcggt gatttcttcg cgtgcggctg gggccggttc gcgccgctca gctcgctcgc
    26941 cgtctgcgtc aatccgggca gcgcatttaa ttgctattgg gaaatgccgt ttcggaagcg
    27001 gtgccggatg acgctgacga atctcgatga taatccgatg gtcctgtatt accagatcaa
    27061 ctacacgctg accgacgtgc cggaggacgc ggcgtatttc cacgcgcagt tccggcgcgc
    27121 caacccgttg ccgtacaagg aggtttacac gatcctggac ggcgtggagg ggtggggaca
    27181 gtatgtcggc acctacatgg catggggggt caacaacaac ggctggtggg gcgaggggga
    27241 gatcaaattc tacatcgacg gcgatgggga atacccgacg atctgcggga cgggaaccga
    27301 ggattatttc tgcggctcct ataatttcga gaatcagacg acgcatcagt atcaggaatt
    27361 ctgcacgccg tatgcggggc tggcgcaggt gatccggccc gacgggctgt atgcctcgca
    27421 gcagcggttc gggctgtatc gctggcacat ccccgatccg atccggttcg agtcggacct
    27481 gcgggtgacg atccaggcgc tggggtggcg gagtcaccgg cgctacctgc cgctgcagga
    27541 cgacattgcg tcggtggcct attggtatca gacgccgatc gcgaagaagt ttccgccgct
    27601 gccgggtcgg gacgagctgg aggtcatttg aagattgagg ggtggggggc gattgggttc
    27661 gccccccagg ttccgctgcg ctgcactcgg gtctaattaa attgcgccac tgcggggcgg
    27721 attcgggggg gttgatgtct ggggcgtcgg tgtggagagc gaagctgcat tcctgggtag
    27781 gccttcggaa gcgaggacga gcgacgtgga gtgaagagag gccggcaagg atgccggcgg
    27841 tcccaggcgg
//
LOCUS       DAOPCO010000026        27779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_1878_length_27779_cov_5.317667, whole genome shotgun sequence.
ACCESSION   DAOPCO010000026 DAOPCO010000000
VERSION     DAOPCO010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 27779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27779
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1878_length_27779_cov_5.317667"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLS90_03650"
     CDS             complement(<1..197)
                     /locus_tag="PLS90_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01531.1"
                     /translation="MSESPPAVPENSADNSIAPHDFDQHSLLRITDPFSRLRVIMKRL
                     LDPGGCPWDREQTHQTLRQYL"
     gene            complement(291..2057)
                     /locus_tag="PLS90_03655"
     CDS             complement(291..2057)
                     /locus_tag="PLS90_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01532.1"
                     /translation="MIRLSLQFPNQPAREMAWDQPTLTIGRAAYNGIVIPAENVSSQH
                     AQICEINGRWFFRDMGSTNGSLLIRDNQKYLLRDHNNEMAIEVGDVLCLASIQNAIAV
                     KWIDGCEEDAEDEDEDQFERTILAEQRNEDEVEFETQAGEEFDALRATVHLSRELVNL
                     ETVNEIARITCATCLQAFPKANRALFLVPQDGKYVIEHAQTSDVDTGDTTSTLMRSRR
                     LLDRCLKERKGFLFLFEANRMQAIATMISAMEQLDEGGTETDRIILCCPLFHLDRNYG
                     FIEVEAPLTPQDRKTLMRRDLSVATLMSHLVAARLHDLENQRARLKLARKATAGFLSA
                     TVGHCFKNLLFVPMSMSKMLPMCIKAGKMEEVEWMLARNSVNIRYLDVLSNEFAAASK
                     DPTEGFGECNIEQLLREVADLVGQINPDKVECRLSVPPDLPPVVCHGAGMKRLLMNLT
                     LNSVDALFGDGKREKGLIELKGAVDLARDELCISVRDNGPGIPGPILENLREIFRQVE
                     ASADALSELQNIAERVRSTKEQGFKEHYGLGFLFVCQTVNHHHGRLEIESTEGHGAKF
                     LISIPLQPVVAGVTQIEEDKSE"
     gene            complement(2523..2897)
                     /locus_tag="PLS90_03660"
     CDS             complement(2523..2897)
                     /locus_tag="PLS90_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01533.1"
                     /translation="MKRVGLAVAVMALVVLTGSLAFAGRSGNSSTWQLPFEVENGKAH
                     VVAPELGGPVDIVVNLKGLDPNVEYVIKSQAQTVGQGFPNKGGNLNLNGTIVDTQGFG
                     RVNLRDANTNALIDSTDMYIVG"
     gene            3222..4508
                     /gene="hflX"
                     /locus_tag="PLS90_03665"
     CDS             3222..4508
                     /gene="hflX"
                     /locus_tag="PLS90_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPK01534.1"
                     /translation="MEEISLEPQKVERAFLVGIQEPGMSREDAEDHLAELASLTDTMG
                     VGVAGQQIARVARPTPQLYLGAGKADEIKALADAHEADVIIFDTDLSPSQQRNWEELT
                     GVAVIDRQEVILDIFAGRAQTREARLQVGLARMEYSLPRLRRAWTHLERQRGGAGMRG
                     GPGELQLEVDRRVVMERITRFKRQLAEVRKQRAVQRQARGARPVPTAAIVGYTNAGKS
                     SLLKRLTGADVFIEDKLFATLDTTTRRILLPNNQPLLLTDTVGFIRKLPHGLVEAFKA
                     TLEEAALADFLVHVLDISNPKIEEHYRVTNEVLEELGAGDRKTIIVLNKADREHDPYL
                     LAQFRRHNPLTFKTSVATGEGIESLLQAFADHFILPMRRLHLRIPHNRFDLVNLVHRE
                     GTVHDQRHEADGVWLDADIPLRHLKDLAPFEPAPPD"
     gene            complement(4847..6913)
                     /locus_tag="PLS90_03670"
     CDS             complement(4847..6913)
                     /locus_tag="PLS90_03670"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPK01535.1"
                     /translation="MSAQPIFQPPYLGSAWYPEAWPHDQMARDVELMKEAGLNVARLG
                     ECIWGRIEPAEQQFDFAWLHEALDRLAEAGIAAILATPTAAPPAWLSARHPEILYVPR
                     DGHGAGQGGRRPVCPSNPVYRAHCRRIIRRLAEEFSGDPRVIGWQIGDGSLPHRAGGG
                     CACPGCLDHFHEFLRARHQTIDRLNELWGLQLWSLAYGDFDQIPAPRVGDHPSLLAAW
                     AEFTSAIHLDFINMQAGVLREVARGEPSSGEAGDSLAGGPRPRRLLGMQQLIGAELPP
                     TGVMDLLEMNRRLDVVQFNHDHPLDQLASAAFWFDRVRPLKGRPFWCTRTAATWTGDV
                     QAGGALPPRGFCRVNSWLPIALGGEANLYGPWRAHWAGPELMHGSVITSDARPGPAFE
                     EIQEISSGFYAAAEFLRNTTVEPARIALHAAHHAWATFQAQPMIAGFDYYDALIRSIY
                     TPLIEAQFRLDVIEPAADLTPYRVVVSPFLPSLDAGELRERLAAWIHAGGTWIAGPLT
                     DIRTRHGTRFTHSPFGSIEEWGGVHLKLSLPGVGGPEILWRDGTLSPAGVWLDGFEPR
                     GAETLATHEGERLKGLAAVTSHRMGQGRVIVLGCLPGGLHWQRLVINAARAAAIAPGI
                     DADPNLLVVPREGDGVGGMIVVELRNKPGSVRLRRKKVDLLSSRRWSGRLDVEPFGVH
                     VLQWDE"
     gene            complement(6942..7898)
                     /locus_tag="PLS90_03675"
     CDS             complement(6942..7898)
                     /locus_tag="PLS90_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01536.1"
                     /translation="MSGWDPVSVVRRAAPCRLRLSLFLSLCLFPLLLLGGCVRAPEPA
                     PYPHESILTVIAELKIFLNEDPYRQPPGRDLEGLNIYRVSLKRLDELMALTGEEYLDI
                     LQFARGESLERLGQYGAAAAAFDRAAAMETALAPMARQRASLARRLADLLDRQAWGAT
                     LDGFLTDLEATIRRLEDWLAESPPWPYDALLQAEIEQLQVERALMLFTNRMVLAGAAE
                     RAISAAEEILQRHQASRRHGEHLLLLGGFFETMARDWLQRERPERRVEAGLDPSVESW
                     ILRARQAYREAAQRDGDPAKPEAQGRLRALDAFDLRMEALAR"
     gene            complement(7895..10285)
                     /locus_tag="PLS90_03680"
     CDS             complement(7895..10285)
                     /locus_tag="PLS90_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK01537.1"
                     /translation="MKALLLPLRSAGRTACLAAFCLAWVAPAFADIVYLHGNRQIEGV
                     VDLDASDAEQVTIRTAAATLRIPRAHIDRIEGAGERSYSEASGDLLAASGDFEKALEQ
                     YQNALAFDPDNAELQRKIESLRGRMQDEEHRAFGRTFDDINRALERQEFEPAIERITG
                     LIERAHSDAIRTHARQLLAQAYVGLARQMRDRVLYPEAERSYRRAIEAYPEGASARLE
                     FADLLQASPARKAEAIQQYAEGIRILEQEGAAGDGAASAEILDYKMKLGRLYQDQNLY
                     AEAAETFLEVTRQDENFRYPDAVELAIDAYASLPVDQLATLADSAVTNLRAIIRERPE
                     DERAYLLLGRIYYNQGQWRQARDLLTQGVARAANKTDARLQDALFALGRSHRELGELE
                     QAAAAINRLVAMRTGHYDALIELGEIRLEQGDPEEAQRLFEEAIQLDPDRYHAYLGLG
                     RALRSLGEYDDARENLEKVVKRDENNAEAQLLIALTYRDEEELERMEIEAEKAVNLVK
                     AQLQENGAAPGAGGDEDEDDDEEITLTDQQAIEMEVTDEQRDVLARAWTMMGEANLKQ
                     KNTNRARELFEMALARQPGHAPAMNGIGVSYQLETRYADAEEWYAKAIAADPDEPDFV
                     LSMAILQHNYLENPGEALPLYLSYLRLGGRDPNVRKWIDDIGGEIPADLEAEIAARGE
                     ETASESASLIELLSTDGGLEPVAGAGLPPAPAEDFATTDGLPGLLPTGPGGQAPPPGP
                     IEGLPAPGTGEVPPPPGPPLGAPGLAPPMPEGAAPAEGAAPAEALPMPPAPAPPAP"
     gene            complement(10613..10987)
                     /locus_tag="PLS90_03685"
     CDS             complement(10613..10987)
                     /locus_tag="PLS90_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPK01538.1"
                     /translation="MNLIDSSAWLEYFIDGPNASLFEPPLKNIEAPVVPIISIYEVFK
                     VLCRESGENIALQGLTAMQRGHVVHLTTELGKKAAVLSLRHALPMADSIILAAAREFD
                     ALIWTQDADFKGLDRVNFFEKK"
     gene            complement(11043..11189)
                     /locus_tag="PLS90_03690"
                     /pseudo
     CDS             complement(11043..11189)
                     /locus_tag="PLS90_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            complement(11367..13844)
                     /locus_tag="PLS90_03695"
     CDS             complement(11367..13844)
                     /locus_tag="PLS90_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK01539.1"
                     /translation="MPKSLMIDPREVRVQGQVEFTPIPINTYNRTMADELAAGAFTRD
                     DLFRIWRDMAIIRAFEMMLQTVKMQGNYEGLEYNHRGPAHLSIGQEAAAVGQCYLLGV
                     DDHIYGSHRSHGEILAKGLSAIHRLDEESLLRIMRGYFDGATLRVVEEGAAGDTRDLA
                     TDFLLYGTLAEIFGREPGFNKGLGGSMHAFFPPFGVYPNNAIVGGSADIAVGAALYKK
                     VNHKPGIVIANIGDSASACGPTWEALCFATMDQYKTLWDAEHRGGLPFIMNFVNNFYG
                     MGGQPVGETMGMRVLARVGAGLTPNQMHSERVDGYNPLAVIDAIRRKREILERGDGPV
                     MLDTLTYRFSGHSPSDASSYREKSEIELWQEIDPLKTFAQQMLEHAVCTQEDLDRAQA
                     RINELILRAYRRSIDLSVSPRANLMPAGNLLERVMFSNERVESMDPGREPEVLLPLAE
                     NPRVQQLAKRSRSGLGDDGQPLPKSRCIGLRDALFEAIIDRFYTDPTLVAYGEENRDW
                     GGAFAVYRGLTEALPYHRLFNTPIAEGAIVGTAVGYALEGGRVIAELMYCDFMGRAGD
                     EIFNQMAKWQSMSGGLLRMPLVLRVSVGSKYGAQHSQDWTSLVAHIPGLKVVFPATPY
                     DAKGLMYSALLGTDPVVFFESQRIYDMPELFHPGGVPEGRYEIPLGEPDVKREGRDLT
                     ILSIGATLYRALEAAQELDEKWGISCEIIDARSIVPFDYEKVIESVKKTRKIVLTSDA
                     CERGSALQTIAARITELAFDELDAPPVVVGARNWITPADEMEEAFFPFPSDMLDAIHT
                     HILPLDGYRPLRDCSVPEMMRRARLGV"
     gene            complement(13911..14711)
                     /locus_tag="PLS90_03700"
     CDS             complement(13911..14711)
                     /locus_tag="PLS90_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPK01540.1"
                     /translation="MSLNTKQRRDQILARLYEAGQVQVKALASLMDVSEATVRRDLRS
                     LADEGHAELFYGGATIPQNSDFSFRSKALRNPEAKRIVGRLAAALVEDGETIFLYGGT
                     TIYEMAHHLRRKRGLSVIANSIRLAAELGSAEGVNLISIGGKYRPDRMDTVGPLAIST
                     LEQLRGYLAFIGSDGLSRDFGITASDIDSAHLHRLAIHNAREAILVVDHTKLRRPSLY
                     RICEFSEISRIVIDSEPPVEWREFFNDLGIEMIFPGREPGPAAKGEPA"
     gene            14899..16881
                     /locus_tag="PLS90_03705"
     CDS             14899..16881
                     /locus_tag="PLS90_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPK01541.1"
                     /translation="MIRITMPQAGQTMEDGTVVRWLKREGDAVAPGDVLLEIETDKST
                     LEVESVAGGTLRRILAPEGTTLPVHAPLAIVGDPNEDIEEAVEQAREELKRQTPADGP
                     APTAPPAPRESPGAKPATAESTPEPDPTAAAAAASVVPILMPQAGQSMEEGTIVKWRV
                     APGAEVKPGDILFEVETDKSVVEVEATDAGRLARILVPEGATVPVKRPVAYLAERDAD
                     VDAWLKAEGAASGAPAGAAPPPAPVSSAPPAGAVVPVLMPQAGQSMEEGTIVKWRVAP
                     GAEVKPGDILFEVETDKSVVEVEATDAGRLARIVVGEGGTIEVKKPVAYLADHDADVD
                     AYLAAGGETNAPEAGDSLPRPVEAHRETAPAAAETPAPAAVEQPLGERPKASPAARKV
                     ARQRGIDLHDVGVGSGPGGRILSTDVATARPTATRHAAERRPTPPASTEPVRRRMSAM
                     RKAIARNLTASKQTIPHFYMRLTIDADPLMKFYRASKAEFPCSLNDVLVLALSRAIRE
                     FPAFRTRIEGEELVEYPFANIGIAVGMEDGLVVPVLQRACCRTLKEIGIETRRLIERA
                     RAGKIEGMGTGVFTVSNLGMFGVDEFSAIINPPESAIMAVSGIREAVIVQEGTLRAGR
                     QMTMTLSCDHRVIDGLVAAQFIARLREMLEQPAEIE"
     gene            17098..18522
                     /gene="lpdA"
                     /locus_tag="PLS90_03710"
     CDS             17098..18522
                     /gene="lpdA"
                     /locus_tag="PLS90_03710"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPK01542.1"
                     /translation="MNDHFNIAVIGTGPGGYIAALKAAQAGASVAVVEQQPYFGGTCL
                     NWGCIPSKTLIASSELLHRLQHAEELGIEISGQVSFSWPKIQQRKDKILNALRGGIRG
                     LFQARKITPFIGKALLDGPGRFVVQPNDGSEPRRFTADQTILAVGSIPSRIPGWPTDP
                     ERVCTSDEALHWRDLPRRLLIVGGGVIGCEFACMLRPFGVEVMLVEMLPNLLTGLDGH
                     LGEAMQAIFSKRGIRVLCEAKVEDLTLTDDGVRAALSNGEQLEFDRVLVATGRRANTA
                     DFGLERVGLTTDRGFIRVDDAMRTEQPDIYCIGDANGRCLLAHAASAQGVVAVESALG
                     QPRAAQAPVPSCVYTFPEVAGVGLTQEGARERGIPIAIGSFPLGHLGKAMAAGETDGF
                     VKVIRHRGSGELLGVHMLGHNVTEVIHAASVLIHTRATARDVAEMIFAHPTIGEGFHE
                     ALEDSLGACLHLPPRKKVRVTVGV"
     gene            18584..19870
                     /locus_tag="PLS90_03715"
     CDS             18584..19870
                     /locus_tag="PLS90_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01543.1"
                     /translation="MPTVTSAALMPLLALEDCLGADQAAAGATSPPPAATANTHIHLP
                     PNFSAFETVARALDLAAEQGVRVLGASNYYDYTVYESFAAGARARSIFPLFGAEIIAM
                     DDILRLSGVKVNDPGNPGKIYFCGKGIVRFDPADQPPAAQYLLEQIRRRDAHRIDEMA
                     RRLADYCAAHGVESPLDAADIHARVVARHACPPATVTLQERHLAQAYQELLFETTPRD
                     RITDRLRALLGAPVEPSDDPAAVQNALRAHLLKSGKPAFVEEDYVTLEEARRLVLELG
                     GIPCYPVLADGTEPITEFESDPETLIAHLRQLRVHCVEFIPVRNRPEVLLRYVRALRA
                     AGFVATAGTEHNTLEPLPVAPTCRDGAPLPAEARAIFWEGACVVAAHQFHAARGEDGF
                     VNARDEPNRRFENDEQRIAAFAGTGARLIEAFCRRA"
     gene            20076..21353
                     /locus_tag="PLS90_03720"
     CDS             20076..21353
                     /locus_tag="PLS90_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK01544.1"
                     /translation="MTDADRQTPQENRAHTDAFGDMARVEPERARRLIDRLGPALRAL
                     LGDATARAVVSFDDSDEARQRVRIVPNPGMTASAPTIPGQTMDDGSHPLWLAAPEEGE
                     DDEALVGRLRQAIAAHMVRYGAAPRAVIIPGLGRFAARRESQPAADRPAGRLAGKVAV
                     VTGAAQGFGREIAAGLAREGALVALADVNQAGVSAAAASLADEVGAGRAHGLAMDVGD
                     ARSIAEALHRVIRLWGGFDIFIANAGVLRAGGVQDLTEAEFDLVTRVNYKGYFLCVRL
                     AARVFALQHRALPGPYGDIIQINSKSGLTGSNRNFAYAGGKFGGIGLTQSFALELIAD
                     RVKVNAICPGNFFDGPLWSDPENGLFVQYLRAGKVPGARTVDDVRRAYEAKIPMGRGC
                     TAADVLKAILYVIDQDYETGQAIPVTGGQVMLS"
     gene            21597..22496
                     /locus_tag="PLS90_03725"
     CDS             21597..22496
                     /locus_tag="PLS90_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPK01545.1"
                     /translation="MAVPPFQDFMLHILQFAGDAKEHTMAEAREHVAKEFGLTQEDRE
                     ELLPSGRVPRFTNRVAWCRVHLQKAGLLESRHRGSFNITPLGLDVLKKQPKRIDLKYL
                     DQFPGHLEFYKRAKKNDGEINEGQPRPPDEVLEESYQELRSELVTALLSALEAASPSF
                     FERVVVELLVRMGYGGSLRDAGQAIGKSGDEGIDGIIKEDRLGLDNVYIQAKKWTAVV
                     GRPEIQKFVGALHGKHAKKGVFITTSSFSSEALEYTRLIDSRVVLIDGVTLAGYMIDF
                     GVGTKLKQVYELKEIDNDYFDEE"
     gene            22522..24207
                     /locus_tag="PLS90_03730"
     CDS             22522..24207
                     /locus_tag="PLS90_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005869465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPK01546.1"
                     /translation="MTTTAIPQKQYAVQLVGPSQLKLNPDKDVPRPGPHQVLAKIEAV
                     GLCFSDLKLLKQFSEHARKKEVVKGLSEQILNGIPSYVPADKPTVPGHEVVLRIVAVG
                     DNVKHHKVGERCIVQADYRNLRTSGSNAAFGYNFEGGLQEYVLMDERVVVDEDGERFL
                     IPVKDDIGASQVALVEPWACVEDSYVTRERQSILAAGRLLVVADAGHAIEGLRESFSP
                     DGPPASITIVCVEDVQLKAIQDLGLPVEEAIDPVALPDEGFDDIVYFGASKPTLDVLN
                     DKLAAHAIINVVKGGKRIGEPVAVGVGRVHYGPTRWIGTDSTRADDAYRNIPPDGDIR
                     DGDSVLVVGAGGPMGQMHVIRNVCAGRKGVSVTGSDLDAARLAALEAKARPMAAANNV
                     SLRMVNSKEEKLDQAFSYYALMAPVGQLVADAIRDGAEGMIINIFAGIPAPVKHDLDL
                     DTYFAKRAFMFGTSGSTIRDMKIVLEKVESGQLNTNASVDAIAGMSGATDGIAAVENR
                     TMAGKIIVYPQLHDVPLIPLSELGEHFPTVAAKLDDGQWTPAAEAELLRVAKA"
     gene            24321..25310
                     /locus_tag="PLS90_03735"
     CDS             24321..25310
                     /locus_tag="PLS90_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011629961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPK01547.1"
                     /translation="MAAPPPKPAVDLLARARRVIELEAEAVAGLTAVLDASFEQAIAR
                     MLAVRGRVVTTGMGKAGLIARKVAATLASTGTPALFVHPAEAVHGDLGMITADDLALA
                     FSHRGQTEEILRILPYLKHVGTPLIAITSNPDSELARHADVAMILPIRQEACPLNLAP
                     TASTTAMLALGDTLALVLLEARGFRPEDYAVFHPGGSLGRRLLTKVGDLMHTGEANPV
                     AHEATPLREALTVMTEKRLAALSLTDDEGRLTGFYTSGDLSRHIGDAGFDVKRPIRDF
                     MTRNPKHARPEMMAVKALEILQQYKIIQLPVCDDAGRPVGMIHLHDITRAGIT"
     gene            25307..25948
                     /gene="rdgB"
                     /locus_tag="PLS90_03740"
     CDS             25307..25948
                     /gene="rdgB"
                     /locus_tag="PLS90_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPK01548.1"
                     /translation="MNAARLLIATGNRHKVDEIAAILRDLLPAFTWELRSAADFPHVP
                     EPVEDGDTFEANAAIKARGWAMHPDVRAVGLAALADDSGLVVDALAGRPGILSARYGA
                     TPTARIERVLREMRDVGDSHRSARFECVVTLASADGDLWTARGTVEGRITHAPRGMAG
                     FGYDPIFELTDGPHAGLTLAEVPESVKNGLSHRARALRALAPTLAAMLRPAGV"
     gene            complement(25990..27519)
                     /gene="rseP"
                     /locus_tag="PLS90_03745"
     CDS             complement(25990..27519)
                     /gene="rseP"
                     /locus_tag="PLS90_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPK01549.1"
                     /translation="MSFTAILDILLGALAILLVFGLAVFVHEFGHMFFALIRGVGVES
                     FAIGMGPKITSWKWRGIEFSLRWIPAGGFVKLKGMMPEELEEPAVAESESAAGDLASG
                     HHEDKELSESAYDDLLALRDEGLVTKLLVFGGGVFMNFVTAIVAAALLGLVGTKAQLF
                     PIELEQVPEASLLAEIGVEPGDRIVAFDGAPTPYSLDLQQAVIEKTGGPMGVGEIYHR
                     FLAGVLPYVEPPDEPTTTALTAMELTVERGGENRRLALEGITNKQLDRLLDEVTLNMR
                     IPPIVGELIPNYPADRAGIKRGDRIVAIDGEPIESFSDMTRLIAARPGVETRITVERE
                     GEQLELTVIPRRDVLKDKPTGQIGVFNGAPDTRVLRETPFRAITLAPVSTMQAFLGML
                     NLNVQFFKRATAKDISQNLGGPIAIAVMTFQRAMGGISELLDWFIMLNLLLMFFNLLP
                     LPVLDGGFIVLSLIEAVIRRPVPTKILAPIYTVFVIFFITLMVAISGLDIWNWFISPL
                     M"
BASE COUNT         4850 a         9429 c         8841 g         4659 t
ORIGIN      
        1 atcagatact ggcggagcgt ctggtgcgtc tgctcgcggt cccatgggca gccgcccgga
       61 tcgagcaagc gcttcatgat cacgcgcagc cggctgaagg ggtcggtgat gcgcaacagc
      121 gaatgctgat cgaaatcgtg cggcgcaatg gaattgtcgg cggagttttc gggaacggcg
      181 gggggggatt cactcatgta tcagacagct tccatcctga atccgacacg caatcggtat
      241 ccgtatcgaa ttcaaacgag atcggcgaac agccggcggc ggcgcggccc ctattcggac
      301 ttgtcttcct cgatctgagt gacgccggcc acgaccggct gcagcggaat ggagatcaga
      361 aacttcgcgc catgcccttc ggtcgattcg atctccagcc ggccgtgatg atggttgacc
      421 gtctggcaga cgaacagaaa gcccaggccg taatgctcct tgaagccctg ctccttcgtg
      481 ctgcggacgc gctcggcgat attctgcagt tcgctcagcg cgtcggcgct cgcctcgacc
      541 tgccggaaaa tctcccgcag gttctccaga atcggccccg ggatgcccgg cccgttgtcg
      601 cgcaccgaaa tgcacagctc atcgcgcgcc agatcgacgg cccccttcag ttcgatcaac
      661 cccttctcgc gtttcccgtc gccgaacagc gcatccaccg aattgagcgt caggttcatc
      721 agcagccgct tcatcccggc gccgtggcag acgaccggcg gcaagtccgg cggcacgctc
      781 aggcggcact ccaccttatc cggattgatc tgcccgacca ggtcggccac ctcccgcagc
      841 aactgctcga tgttgcattc gccgaatccc tcggtcgggt ccttgctcgc cgcggcgaac
      901 tcattcgaga gcacgtccag atagcggatg ttgacgctgt tgcgggccag catccactcc
      961 acctcctcca tcttgccggc cttgatgcac atcggcagca tcttgctcat cgacatcggc
     1021 acgaacagca ggttcttgaa gcaatgcccc accgtggccg agaggaaacc ggccgtcgcc
     1081 ttgcgcgcca gcttcagccg cgcccgctga ttctccagat cgtgcagccg cgccgccacc
     1141 aggtggctca tcagcgtcgc caccgacagg tcgcgccgca tcagcgtctt gcggtcctgc
     1201 ggcgtcagcg gcgcctccac ctcgatgaac ccgtaattgc ggtccagatg gaacagcggg
     1261 cagcacagga tgatccggtc cgtctcggtc cctccctcgt cgagctgctc catcgccgaa
     1321 atcatcgtcg cgatcgcctg catgcggttc gcctcgaaca ggaacaggaa gcccttgcgc
     1381 tctttcagac agcggtccag caaccggcgc gaccgcatca gcgtcgacgt cgtatccccc
     1441 gtgtccacgt cgctcgtctg ggcatgctcg atgacgtact tgccatcctg cggcaccagg
     1501 aacaatgcgc gattggcctt cggaaacgcc tgcaggcagg ttgcgcaggt gatccgcgcg
     1561 atttcgttga ccgtctcgag gttcaccagt tcgcgcgaca ggtggaccgt cgcccgcagg
     1621 gcgtcgaact cctcgcccgc ctgcgtctcg aactccacct cgtcctcatt gcgctgctcg
     1681 gccaggatcg tccgctcaaa ctgatcctcg tcctcgtcct ccgcgtcctc ctcgcagccg
     1741 tcgatccact tgaccgcgat cgcgttctga atgctggcca ggcacaggac gtcgcccact
     1801 tcgatcgcca tctcgttgtt gtggtcgcgc agcaggtatt tctggttgtc gcggatcagc
     1861 agcgagccgt tggtgctgcc catgtcgcgg aaaaaccacc ggccgttgat ctcgcagatc
     1921 tgcgcatgct ggctcgagac attctccgcc gggatgacga tcccgttata ggcggcgcgg
     1981 ccgatcgtca gcgtcggttg atcccacgcc atctcacgcg ccggctgatt gggaaactga
     2041 agcgacaggc gtatcatcgc aactgctggc cctcttgatc cccttgaatc cgtctctcgt
     2101 ccctcgtcct cgaccctcgc aaacccgcga tcgccatatc ccccagctcc cgtaagccgc
     2161 ccatccctga gcaaatcctc ctcggctcca tggagcagcg gagcctcggg cacaactccc
     2221 ctacagatcg gtcttcatct tatgggaact gcgccggctg atcaagtccg cgaaatttgc
     2281 ccaaattgcc tcttcatggc gactttgcgt tatcgcatct ccgcgccgac tcccgttcga
     2341 acccatcggc gcttggctgg cagggccggc ggagtgggcg aaccggcgat tgcatccgat
     2401 aatgacgcgg aaaaggacag gcacggtcgg ggaaaggcgt ggcggcaatt ggggcgattg
     2461 gccggaacgg tgggatagac ccaaccgttc cggcctctgc gactcggaag attcacgatc
     2521 atttagccaa caatgtacat atccgtactg tcaatgagtg cgttggtatt ggcatcacgc
     2581 aggttgactc ggccgaatcc ctgcgtatcg acgatcgttc cattcagatt gaggtttcct
     2641 cccttgttgg gaaacccctg tccgaccgtc tgagcttgcg acttgatcac gtattccacg
     2701 tttggatcaa gccccttcag gttcacgacg atatcgaccg gcccgcccag ttccggcgcc
     2761 acgacatgcg ccttgccgtt ctcgacttca aacggcagtt gccatgtcga tgagttgcct
     2821 gaacgcccgg cgaacgccag tgaccccgtc agcaccacga gagccattac agctacagcc
     2881 agaccgaccc gtttcatagt cgtcctcctg tgaacaggat gaatagatgg tcgttggttg
     2941 ctcttccgag tatgcgcgtc attctccgcc ttcccgccca aaccgtcaaa gtaaattgca
     3001 tcacccccgt ttggtctcca atacgtccca cgtcctcatc tgcgtccccc gtctctcgaa
     3061 atcccgccgc catcggacgg tgctcgcgca cccgcaggcc gcgcctgaac accggccgac
     3121 gaactcgctc tcatgtcgcg ccatcctgtg ctagcatcgc cttgaagccg ttgcagggct
     3181 tcgcccgcgg aggcccactc cgctctcgca tggattcgcc gatggaagaa atttcactcg
     3241 aaccgcaaaa agtcgaacgc gcgttcctcg tcggcattca ggagcccggc atgtcgcgtg
     3301 aggacgccga ggaccacctc gccgaactgg ccagcctgac cgacacgatg ggggtcggcg
     3361 tcgccggcca gcagatcgcc cgcgtcgcgc gcccgacgcc gcagctctac ctcggcgccg
     3421 gcaaggccga cgagatcaag gccctcgccg acgcccacga ggccgacgtt atcattttcg
     3481 ataccgacct cagcccctcc cagcagcgca actgggagga actgaccggc gtggccgtca
     3541 tcgaccgcca ggaagtgatt ctggatattt tcgccggccg cgcgcagacc cgcgaggccc
     3601 gtctccaggt cggcctggcg cggatggaat actcgctccc ccggctgcga cgcgcgtgga
     3661 cccatctgga gcgccagcgc ggcggcgcgg ggatgcgcgg cggccccggc gagctccagc
     3721 tcgaggtcga ccgccgcgtc gtcatggaac gcatcacccg cttcaaacgc cagctcgccg
     3781 aggtgcgcaa gcagcgcgcc gtccagcgcc aggcccgcgg cgcacgcccc gtccccaccg
     3841 ccgccatcgt gggctacacc aatgccggca agtcctcgct gctcaagcgc ctgaccggcg
     3901 ccgatgtctt catcgaggac aagctgttcg ccacgctcga caccaccacg cgccgcatcc
     3961 tgctccccaa caaccagccg ctcctgctga ccgacacggt cggcttcatc cgcaagctcc
     4021 cccacggtct ggtcgaagcc ttcaaggcga cgctggagga agccgcgctg gccgacttcc
     4081 tcgtgcatgt cctcgacatc tcgaacccca aaatcgaaga gcactaccgc gtgaccaacg
     4141 aggtgctcga ggaactcggc gcgggcgacc gcaagacgat catcgtcctg aacaaggccg
     4201 accgcgagca cgacccctac ctgctggcgc aattccgccg gcacaacccg ctgacattca
     4261 agacgtcggt cgcgaccggc gagggcatcg agtcgctcct ccaggccttt gccgaccact
     4321 tcatcctgcc gatgcgccgc ctgcatctgc gcatcccgca caaccgcttc gacctggtca
     4381 acctggttca ccgcgaggga accgtccacg accagcgcca cgaggccgat ggcgtctggc
     4441 tcgacgccga catcccgctg cgccacctca aggacctcgc cccgttcgag cccgccccgc
     4501 ccgactgacc catgttggag cgcggtgcca gagcattgca acgcaacgcg tcgaccccgc
     4561 tttggatcga ttacccgtag ccgattctga actttctcca ggtaacgtcg tggattgatt
     4621 tcgtctgagg gcgttccaac ccacggttgg tatgttcttc tccgcgtccc ggcgtctcgg
     4681 cgtgagattc cttcccccag caaaaaactc cgccgacgtc gccaaggagt agatgcaaga
     4741 aaacgagccg acggtatacg tcttacaccg agccgcagag ccacgaaaga agtcgatcga
     4801 ttccgacctc cgcgattctc gccctcgaat ccatacccct gcgcccctac tcgtcccact
     4861 gcagcacatg cacgccgaac ggttccacat caagtcggcc cgaccagcgg cggctgctga
     4921 gcaggtccac tttcttgcgt cgcaggcgga ccgatcccgg tttgttgcgc agttcgacaa
     4981 cgatcatgcc cccgaccccg tccccctcgc gcggaacgac caacaggttg gggtcggcgt
     5041 cgatgccggg ggcaatcgcc gccgcgcggg cggcattgat caccagccgc tgccagtgca
     5101 gtccgccggg caggcagccc agcacgatga cgcgcccctg gcccatccgg tggctggtga
     5161 ccgccgccag gcctttgagc cgctcccctt cgtgcgtggc gagcgtctcg gcgccgcgcg
     5221 gctcaaaccc atcgagccac acgccggccg gcgagagcgt gccgtcgcgc cagagaatct
     5281 cgggcccgcc cacaccgggg aggctgagtt tcagatgaac gcccccccac tcctcaatcg
     5341 acccgaacgg tgagtgggtg aaccgggtgc cgtggcgcgt gcggatgtcg gtcagcgggc
     5401 cggcgatcca ggtccccccg gcgtggatcc acgccgccag ccgttcgcgc agttcgcccg
     5461 catcgagcga cggcagaaac gggctgacaa cgacccgata cggggtcagg tcggcggccg
     5521 gctcgatcac atccagccgg aactgcgcct cgatcagcgg cgtgtagatc gagcggatga
     5581 gcgcatcata gtaatcaaac ccggcgatca tcggctgtgc ctgaaacgtt gcccaggcgt
     5641 gatgcgcggc atgcagtgcg atgcgcgccg gttcaacggt cgtgttgcgc aggaattcgg
     5701 cggcggcgta gaacccgctg gagatctcct ggatttcctc gaaggcgggg ccggggcgcg
     5761 cgtcgctggt gatgaccgag ccatgcataa gttccggacc ggcccagtgg gcgcgccacg
     5821 gcccgtaaag attggcctcg ccccccagcg cgataggaag ccaactgttc acccggcaga
     5881 acccgcgcgg cggcagcgcc ccgcccgcct ggacgtcgcc ggtccaggtg gcggcggttc
     5941 gggtgcacca gaaggggcgc cccttgagcg gacgcacgcg gtcgaaccag aaggcggcgc
     6001 tcgccagctg gtcgagcggg tgatcgtgat tgaactgcac gacatccagc cgccgattca
     6061 tctcgagcag atccatcaca ccggtcggcg gcagctcggc gccgatgagc tgctgcatgc
     6121 cgagcagccg gcgcgggcgc ggtccgccgg cgagggaatc gcccgcctcc ccgctcgatg
     6181 gttcgccgcg cgccacctcg cgcagcaccc cggcctgcat gttgatgaaa tcgaggtgaa
     6241 tcgccgaggt gaattcggcc caggccgcca gcagcgaagg atgatccccg acgcgcggcg
     6301 ccgggatctg gtcgaaatcg ccgtaggcca ggctccagag ctgcaggccc cacagttcgt
     6361 tcaggcggtc gatcgtctga tgccgggcgc gcaggaattc atggaagtga tcgagacagc
     6421 ccgggcaggc gcagccgcct ccggcacggt ggggtaggct tccgtcgccg atctgccagc
     6481 cgataacgcg cgggtcgccg ctgaactcct ccgcgaggcg gcgaataatc cgccggcagt
     6541 gcgcgcgata aacaggattg gacgggcaga ccgggcggcg gccgccctgg ccggcgccgt
     6601 gcccgtcgcg gggaacatag aggatctcgg ggtggcgggc gctgagccag gccggcggcg
     6661 cggcggtggg cgtcgcgagg atggccgcga tcccggcctc ggcgagccgg tccagcgcct
     6721 cgtgcaacca ggcgaagtcg aactgctgct cggccggctc gatccgcccc cagatgcatt
     6781 cccccagccg ggcgacattg agcccggcct ccttcatcaa ctccacatcg cgcgccatct
     6841 gatcgtgcgg ccacgcctcg ggataccagg ccgaaccaag atacggcggc tgaaagatcg
     6901 gctgagcgct catcggcgac gctttccagg ccgttgtagg cctagcgggc gagggcttcc
     6961 atgcgcaggt caaaggcatc cagcgcgcgc agccgtccct gcgcctcggg cttggccggg
     7021 tcgccgtcgc gctgggcggc ctcgcggtag gcctggcgcg cgcgcaggat ccaactttcc
     7081 accgacgggt ccagaccggc ttcgacgcgc cgctcgggcc gctcgcgttg aagccagtcg
     7141 cgcgccatcg tttcaaaaaa cccgcccagc agcagcaggt gctcgccgtg gcgccggctg
     7201 gcctggtggc gctggaggat ctcctcggcg gcggagatcg cccgctcggc agcgccggcc
     7261 agcaccatcc ggttcgtgaa cagcatcagc gcccgctcga cctgcaattg ctcgatctcg
     7321 gcctgcaaca aggcgtcgta gggccagggc ggtgattcgg cgagccaatc ctccagccgg
     7381 cggatcgtcg cttccaggtc ggtgaggaac ccatccagcg tggcgcccca tgcctggcgg
     7441 tcgagcaggt cggcgagccg gcgggccagg ctcgcgcgct ggcgggccat cggcgccagc
     7501 gccgtctcca tcgcggcggc gcggtcgaag gcggcggcgg ctgcgccata ctggccgagg
     7561 cgctccaggc tctccccccg cgcgaattgc aggatgtcca gatactcctc gcccgtcagc
     7621 gccatcaact catcgagccg cttgagcgag acccgataga tgttgagccc ttccaggtcg
     7681 cggcccggag gttggcgata ggggtcctcg ttgaggaaga tcttcagttc ggcgatgacg
     7741 gtcaggatcg actcgtgggg gtacggcgcc ggctcgggcg cgcgcacgca gccgccgagc
     7801 aacagcagcg ggaaaagaca aaggctcagg aacaggctga gccggagccg gcagggcgcg
     7861 gcacggcgga caacggaaac cgggtcccag ccgctcacgg ggcgggcgga gcgggcgccg
     7921 gcggcatcgg caaggcctcc gccggcgcgg cgccttcggc cggcgcagcg ccctccggca
     7981 tcggcggcgc caggcccggc gcccccaacg gcgggccggg gggcggggga acttcgccgg
     8041 tccccggggc gggcaaccct tcgatcggcc cgggcggcgg cgcctggccg ccgggtccgg
     8101 tcggcaggag cccgggcaag ccgtcggtcg tggcaaaatc ctcggcggga gccggtggca
     8161 atcccgcgcc ggccaccggc tccaggccgc cgtcggtact cagcagctcg atcaggctcg
     8221 ccgattcact cgcggtttcc tcgccgcggg cggcaatctc ggcctccaga tcggccggga
     8281 tctcgccgcc gatgtcgtcg atccatttgc gcacattggg atcgcggccg cccaggcgca
     8341 ggtagctcag atagagcggc agcgcctcgc cggggttctc gaggtaatta tgctgcagga
     8401 tcgccatgct gaggacgaaa tccggctcgt ccgggtccgc cgcgatggcc ttggcatacc
     8461 attcctcggc gtcggcgtag cgcgtctcga gctgatacga aacgccgatg ccgttcatcg
     8521 ccggggcatg acccggctgg cgggccagcg ccatctcgaa cagctcccgc gcgcgattgg
     8581 tgttcttctg tttaaggttg gcttcgccca tcatcgtcca tgcgcgcgcc agcacgtcgc
     8641 gctgctcgtc ggtcacctcc atctctatcg cctgctggtc ggtcagtgta atctcttcgt
     8701 cgtcgtcctc atcctcatcg ccgccggcgc cgggcgccgc gccgttctcc tgaagctgag
     8761 ccttgaccag attgacggcc ttctccgcct caatttccat ccgctccagt tcttcctcgt
     8821 cacggtaggt caacgcgatc agcagctgcg cctcggcgtt gttctcgtcg cgcttgacca
     8881 ccttctccag gttctcgcgg gcatcgtcgt actcgcccag gctgcgcagg gcgcggccca
     8941 atcccaggta ggcatgatag cgatccgggt cgagctggat ggcttcctcg aacagccgct
     9001 gggcctcttc cggatcgccc tgttcgagcc tgatttcgcc tagctcgatc agcgcgtcgt
     9061 aatggcccgt ccgcatcgcg acgagccgat tgatcgccgc ggcggcctgt tcgagctcgc
     9121 ccagttcgcg gtgcgagcgg cccagggcga acagcgcgtc ctgcagccgc gcatcggtct
     9181 tgttcgcggc ccgcgcgaca ccctgcgtca gcaggtcgcg cgcttggcgc cactgaccct
     9241 ggttatagta gatccgcccc agcaacagat aggcccgctc atcttcgggc cgttcccgga
     9301 tgatcgcccg caggttggtg acggccgaat cggcgagcgt cgcaagctga tccaccggca
     9361 ggctggcata ggcatcgatt gccagctcga ccgcgtccgg atatcggaaa ttctcatcct
     9421 ggcgggtcac ctcgaggaac gtttccgccg cctcggcata aagattctgg tcctgataga
     9481 gccggcccag tttcattttg taatccagga tctcggcgct cgccgcgccg tcgcccgccg
     9541 ccccctcttg ttccaggatc cgaatcccct cggcatactg ctggatggcc tcggccttgc
     9601 gcgcgggcga ggcctgcagc aggtcggcga attcgagccg ggcgctggcc ccttccggat
     9661 acgcctcgat ggcgcggcgg taactgcgct cggcttcggg atacagcacg cggtcgcgca
     9721 tctggcgcgc aaggccgaca tacgcctgcg ccagcagttg acgggcgtgc gtccggatcg
     9781 cgtcggagtg ggcgcgctcg atcaggccgg tgattcgctc gatcgccggt tcgaactcct
     9841 ggcgctccag cgcgcggttg atgtcgtcga acgtgcggcc gaatgcacga tgctcctcgt
     9901 cctgcatgcg gccgcgcagc gactcgatct tgcgctgcaa ctcggcgttg tccgggtcga
     9961 acgccagggc gttctgatac tgttcgagcg ctttttcgaa gtcgccgctc gccgccagca
    10021 ggtcgccgct ggcttcgctg taactccgct cgcccgctcc ttcgatccgg tcgatgtggg
    10081 cccgcgggat gcgcagcgtg gccgccgccg ttcggatcgt gacctgctcg gcgtcagagg
    10141 catccagatc gacgaccccc tcgatctgcc gattgccgtg caggtagaca atgtcggcga
    10201 acgccggcgc gacccacgcc aggcagaatg ccgcgagaca ggccgtccgg cccgcggacc
    10261 gcaggggaag caagagagct ttcatgagaa ccgcatgtga gtgggaatca atccatgggc
    10321 gacccggccc cgccgcgggg gcggagccga tgactcgcag ctctgatctt aggcaaaccc
    10381 cgggaaacgg tcaaccagcg ggatgggggg agatttggag agacagagga ggttcctaca
    10441 acggcgctgg ctcgtcaagg ttccttggga ggagtgaatc ccctagccga ggacgtcccc
    10501 cttcgcctcg cattttggtg tttgttgtcc aaccgaccgc tgccagacat tccgggccgc
    10561 gaaattctcg acccctcatc cctcgttgaa ccgcaggctc tggtgcgact ggttacttct
    10621 tctcaaagaa attgactcga tccaatccct taaaatcagc atcctgcgtc cagatcaacg
    10681 catcgaattc acgcgccgcg gccagaataa tgctgtcggc catcggcaga gcgtgccgaa
    10741 ggctgagaac cgccgccttc ttaccaagct ccgttgtcag atgaaccacg tggccgcgct
    10801 gcatggcggt cagcccctga agagcgatgt tttcgcccga ttcccgacac aggactttga
    10861 aaacctcata aatcgagatg atcgggacga ccggggcctc aatattcttc agcggaggtt
    10921 cgaagagtga ggcgttgggt ccatcgatga agtattcgag ccaggcggat gagtcgatga
    10981 ggttcacggg cgatcactgt cgcgttcaat tcgacgttga tcccctttac ataacttcgc
    11041 tattcgataa tgtcccgctg cggaatgaac tccaaccgcc cttgatgctc aatcacctgc
    11101 atcctctgtc ccgggcggat cttcagcctt tccctgacag agcgcggaat cacaacttgg
    11161 aatttggggg atacgataac cgtttccatg tcgccttccc gtccctctcg gcttggttcc
    11221 aggatagagg aacccctgtt gatcaagtcc gcgacacagt gaaggaggga gcagcagcga
    11281 gaggaggcat agggaagggc ttcgtggtga tctccgcggc cgctgtggaa atcttttctg
    11341 ctgggtgcgg ccgctttagc accgccttac acccccagcc gcgcgcggcg catcatttcg
    11401 ggaaccgaac agtcgcgcag cggccggtag ccgtcgagcg gcaggatgtg cgtgtggatc
    11461 gcgtcgagca tgtcgctcgg gaaggggaag aacgcttcct ccatctcgtc ggcgggggtg
    11521 atccagttgc gcgcgccgac gacgaccggc ggggcgtcca gctcgtcgaa cgccagctcg
    11581 gtgatgcgcg cggcgatcgt ctgcagcgcc gagccgcgtt cgcaggcgtc ggacgtcagc
    11641 acgatcttgc gcgtcttctt gaccgactcg atcaccttct cgtaatcgaa cggcacgatc
    11701 gagcgcgcat cgatgatttc gcaggagatc ccccacttct cgtcaagctc ctgcgcggcc
    11761 tccagcgcgc ggtagagcgt cgcaccgatg ctcaggatcg tcaggtcgcg cccctcgcgc
    11821 ttcacgtccg gctcgcccag cgggatctcg tagcgcccct cgggcacgcc gccggggtgg
    11881 aacagttcgg gcatgtcgta gatccgctgc gactcgaaga agacgaccgg atcggtcccc
    11941 agcagggccg aatacatcag ccccttggcg tcgtacggtg tggcggggaa gacgaccttc
    12001 agccccggga tgtgggccac cagcgaggtc cagtcctggc tgtgctgcgc cccgtatttg
    12061 gagccgacgc tgacgcgcag caccagcggc atgcgcagca gcccgcccga catcgactgc
    12121 catttggcca tctggttgaa gatttcgtcg ccggcgcggc ccatgaagtc gcaatacatc
    12181 agctccgcaa tgacccgtcc cccctccagc gcatagccga cggccgtgcc gacgatggcc
    12241 ccctcggcaa tcggcgtgtt gaacaggcgg tgatagggca gcgcctcggt cagcccgcga
    12301 tagacggcga aggcccctcc ccagtcgcgg ttttcttcgc cataggcgac cagcgtcggg
    12361 tcggtgtaaa agcggtcgat gatggcctcg aaaagcgcgt cgcgcaggcc gatgcagcgg
    12421 ctcttgggga gcggctgtcc gtcgtcgccc agccccgagc ggctgcgctt ggcaagctgc
    12481 tgcacgcgcg gattctcggc cagcggcagc aggacttccg gttccctccc cgggtccatc
    12541 gattcaactc gctcattgct gaacatcacc cgctccagca ggttgccggc cggcatcagg
    12601 ttggcgcgcg gcgagaccga caggtcgatc gacctgcggt aggccctcag aatcagttcg
    12661 ttgatccgcg cctgcgcccg gtccagatcc tcctgcgtgc agaccgcgtg ctccagcatc
    12721 tgctgggcga atgtcttgag cggatcgatc tcctgccaca gttcgatctc gcttttttcg
    12781 cgataggagc tggcgtcgga cggcgaatgg ccgctgaaac ggtaggtcag cgtatcgagc
    12841 atcaccgggc cgtcgccgcg ctccaggatc tcgcgcttgc ggcggatggc gtcgatgacg
    12901 gccagcggat tgtagccgtc gacccgctcc gagtgcatct gattgggggt gaggccggcg
    12961 ccgacgcgcg ccaggacgcg catccccatc gtctcgccga cgggctggcc ccccatccca
    13021 tagaaattgt tcacgaagtt cataatgaac ggcagaccgc cgcggtgctc cgcgtcccac
    13081 agcgttttgt actggtccat cgtcgcgaag cacagggctt cccacgtcgg gccgcaggcc
    13141 gacgcggagt cgccgatgtt ggcgatgacg atccccggct tgtgattgac cttcttgtag
    13201 agcgcggccc cgacggcgat gtcggccgac ccgccgacga tcgcgttgtt cggatagacg
    13261 ccaaacggcg ggaagaaggc gtgcatcgag ccgccgagcc ccttgttgaa gcccggttcg
    13321 cggccaaaaa tctcggcgag cgtgccgtag agcagaaaat cggtcgccag gtcgcgcgtg
    13381 tcccccgcgg ccccctcctc caccacacgg agcgtcgcgc cgtcgaagta accccgcatg
    13441 atgcgcagca gggattcctc atcgagccga tggatcgccg aaagcccctt cgcgaggatc
    13501 tcgccgtggc tgcggtgact gccgtaaatg tggtcgtcga cccccagcag gtagcactgg
    13561 ccgacggcgg ccgcctcctg gccgatcgac aggtgcgccg gcccgcggtg gttgtattcc
    13621 agcccctcgt agttgccctg catcttcacg gtctggagca tcatctcgaa ggcgcggatg
    13681 atcgccatgt cgcgccagat gcggaacagg tcgtcgcggg tgaacgcgcc ggcggccagt
    13741 tcatcggcca tcgtgcggtt gtaggtgttg atcgggatcg gcgtgaattc cacctgaccc
    13801 tgaacgcgca cttcgcgggg atcgatcatc aggctcttgg gcatggctgg aggctccttg
    13861 gctgattcag gatacggaaa gccggcgagg cacgaaattc tgcgcgcggg tcatgcgggc
    13921 tcgcctttgg cggcgggccc gggctcgcgg ccggggaaga tcatctcgat ccccaggtcg
    13981 ttgaagaact cgcgccattc gaccggcggt tcgctgtcga tcacgatgcg cgagatctcg
    14041 gaaaattcgc agatccggta gagcgacggg cggcgcagct tggtgtgatc cacaaccagg
    14101 atcgcctcgc gggcgttgtg gatcgccagc cggtgcaggt gcgcgctgtc gatgtcgctg
    14161 gcggtgatcc cgaaatcgcg gctcagcccg tctgatccga tgaaggcaag gtagccgcgc
    14221 agttgctcca gcgtgctgat cgccagcggg ccgaccgtat ccatgcggtc agggcggtat
    14281 ttacccccga tgctgatcag gttgacccct tcggcgctcc ccagctcggc ggccaggcgg
    14341 atcgaattgg cgatgaccga cagcccccgc ttgcgccgca ggtgatgggc catctcgtag
    14401 atcgtcgtgc cgccgtagag gaagatcgtt tcgccatcct cgacgagcgc ggcggccagc
    14461 cgcccgacga tgcgcttggc ctcggggttg cgcagggcct tggagcggaa cgaaaagtcg
    14521 ctgttctggg ggatcgtggc gccgccgtag aacagctcgg cgtgcccttc atcggccagc
    14581 gagcgcaggt cgcgccggac ggtggcttcc gacacgtcca tcagcgacgc cagagccttg
    14641 acctgcacct ggcccgcttc gtagaggcgc gcgaggatct ggtcgcggcg ttgcttggtg
    14701 ttgaggctca tcgaggactc tgtttcgcga agccgggatt cgggccagcc aacaaacgac
    14761 aagattggac aggaaacaag catgaatttc aagaataatt tgcaggattg cgcttgacgc
    14821 ccgcgccgca cggcgctagg gtgaccttgg cctgttccca ccggctcgcc caaccgaacc
    14881 cttccggaag acataccgat gatccgcatc accatgccgc aagcgggcca gacgatggag
    14941 gatggaacgg tcgtccgctg gctcaagcgc gagggggacg ccgtcgcgcc cggcgacgtg
    15001 ctgctggaga tcgaaaccga caagtccacc ctggaggtgg agtccgtggc cggcgggacc
    15061 ttgcgccgca tcctggcgcc cgaggggacc acgctcccgg tgcatgcgcc gctcgcgatc
    15121 gtgggcgacc cgaatgaaga tatcgaggag gcggtggagc aggcgcgcga ggaattgaag
    15181 cgacagacgc cggcggacgg cccggcgccg accgcgcccc ctgccccgcg ggaatcgccc
    15241 ggcgcgaaac cggcgaccgc cgagtccacc ccggagccgg accccacggc cgccgccgcc
    15301 gcggcaagcg tcgtccccat tctgatgccg caggccggcc agtcaatgga agaggggacg
    15361 atcgtcaagt ggcgcgtcgc gcccggcgcc gaagtgaagc cgggcgacat cctgttcgag
    15421 gtggagaccg acaaatcggt cgtggaagtg gaggcgaccg acgccggccg gctcgcgcgg
    15481 atcctcgtgc ccgagggcgc aacggtcccc gtgaagcgcc cggtcgccta cctggcggag
    15541 cgcgacgccg acgtggatgc ctggctgaag gccgaaggcg cggcgagcgg cgctccggcg
    15601 ggcgcggcgc cgcccccggc cccggtcagc tcggcgcctc cggccggagc agtcgtgccg
    15661 gtcctgatgc cgcaggccgg ccagtcgatg gaagagggaa cgatcgtcaa gtggcgcgtc
    15721 gcgcccggcg ccgaagtgaa gccgggcgac atcctgttcg aggtggagac cgacaaatcg
    15781 gtcgtggaag tggaggcgac cgacgccggc cggctggcgc ggatcgtcgt cggcgaaggc
    15841 ggcacgatcg aggtcaaaaa gccggtggcg tatctggccg accacgacgc cgacgtcgac
    15901 gcctatctgg cggcgggcgg cgagaccaat gcacccgaag cgggcgacag cctgccgcgg
    15961 ccggtcgagg cgcatcggga gacggctccc gccgcggccg aaacgcctgc accggcggcc
    16021 gttgaacaac cgcttggcga acggcccaag gcatcgccgg cggcgcgcaa ggtcgcccgc
    16081 cagcgcggca tcgatctgca cgatgtcggc gtgggcagcg gcccgggcgg acggatcctg
    16141 tcgaccgacg tggcgacggc gcggccgacg gcgacgcgcc acgccgccga gcgccggccg
    16201 accccgccgg cctcaaccga gccggtccgc cggcggatga gcgcgatgcg caaggcgatc
    16261 gcccgcaacc tgaccgcctc caagcagacg atcccgcatt tctacatgcg gctgacgatc
    16321 gacgccgatc cgctgatgaa gttttaccgg gcgtccaagg ccgaattccc gtgcagcctg
    16381 aacgacgtcc tcgtcctcgc cctctcccgg gcgatccgcg agttccccgc cttccgcacg
    16441 cggatcgagg gcgaggaact ggttgaatac cccttcgcga acatcggcat cgccgtcggc
    16501 atggaggatg gactggtcgt gccggtcctg cagcgcgcct gctgccggac gctgaaggag
    16561 atcggcatcg aaacgcggcg tctgatcgaa cgcgcccgcg ccggcaagat cgaggggatg
    16621 ggaacgggcg tcttcaccgt cagcaacctg ggcatgttcg gcgtcgacga attttcggcg
    16681 atcatcaatc cgccggaatc ggcgatcatg gccgtgagcg gcatccgcga agccgtgatc
    16741 gtgcaggaag ggacgctgcg cgcggggcgc cagatgacga tgacgctgag ttgcgaccac
    16801 cgcgtgatcg acggcctcgt cgcggcgcag ttcatcgccc ggctgcgcga gatgctggag
    16861 caaccggcgg aaatcgaatg aggaaaatcg aaatacgaca aaatgaaaat acagactatt
    16921 ccatgcgcga aatcccaggt ggatacaaat tccaatgatt aaaaatcaaa atataaatcc
    16981 gtttcgccct cgtttccaat ttgaaattct ttcgtatttc gtgcttcgaa tttcgaattt
    17041 cttcttgaag cttcttgagg cttggccgcg caatttcaat gggagcgtcc ccacggcatg
    17101 aacgatcact ttaacatcgc cgtgattggc accggccccg gcggctatat tgcagcgctc
    17161 aaggcggcgc aggcgggcgc ctcggtcgcc gtcgtcgagc agcagcccta tttcggcggc
    17221 acgtgcctca actggggttg catcccctcc aagacgctca tcgcctcaag cgaactgctc
    17281 catcgcctgc agcatgccga ggagttgggc atcgaaatct ccggccaggt gtcgttcagt
    17341 tggccgaaaa tccagcagcg caaggacaaa atcctcaacg cgctgcgcgg cggaatccgc
    17401 ggcctgttcc aggcgcgcaa gatcaccccc ttcatcggca aggccctcct ggacggcccc
    17461 gggcggttcg tcgtgcaacc gaacgacggg tcggagccgc gccgcttcac ggccgatcag
    17521 acgatcctcg ccgtcggcag catcccctcg cgcatccccg gctggccgac cgatcccgag
    17581 cgcgtctgca cgagcgatga ggcgctgcac tggcgcgacc tcccccggcg cctgctgatt
    17641 gtcggcggcg gcgtcatcgg ctgcgagttc gcctgcatgc tccggccgtt cggcgtcgag
    17701 gtgatgctcg tcgagatgct ccccaacctg ctgaccggcc tggacggcca tctgggcgaa
    17761 gccatgcagg cgatcttcag caagcgcggc atccgcgtgc tgtgcgaggc gaaggtggaa
    17821 gacctgacgc tgaccgacga cggcgtccgc gccgcgctga gcaatggcga gcagctcgaa
    17881 ttcgaccgcg tcctcgtcgc cacgggccgc cgggccaaca cggccgattt cggcctcgag
    17941 cgcgtcggcc tcacgaccga ccgcgggttc atccgcgtcg acgatgcaat gcggaccgag
    18001 cagcccgaca tctactgcat cggcgacgcg aacggccgct gtctgctggc gcacgccgcg
    18061 tcggcccagg gcgtcgtcgc ggtcgagtcg gcgctgggcc agccccgcgc ggcgcaggcc
    18121 cccgtcccct cctgcgtcta cacgttcccc gaagtggcgg gcgtcggcct cacgcaggaa
    18181 ggcgcgcgcg aacggggcat tccgattgcg atcggctcct tccccctggg gcatctgggc
    18241 aaggcgatgg cggcgggcga gaccgacggt ttcgtcaagg tcatccggca tcgcggttcc
    18301 ggcgagctgc tcggcgtgca catgctgggg cacaacgtga ccgaagtgat tcacgccgcc
    18361 tcggtattaa tccatacccg ggcgacggcg cgcgatgtgg ccgagatgat cttcgcgcat
    18421 ccgaccatcg gcgagggctt ccacgaagcg ctggaagaca gcctgggggc ctgcctgcac
    18481 ctgccgccgc gcaagaaggt gcgcgtgacg gtgggggttt aacttagcag cgagtgcgct
    18541 ccgccgaacg cggatcggaa attgaagccc gccgggaacc gccatgccaa ccgtaacctc
    18601 cgccgcgctg atgcccctgc tggcgctcga ggactgcctg ggcgccgacc aggcggcggc
    18661 cggcgcgaca tcgcctccgc ccgccgcgac ggcgaacacc cacattcacc tgccgccgaa
    18721 tttctcggcg ttcgagacgg tggcgcgggc gctcgatctc gcggccgaac agggcgtgcg
    18781 cgtcctgggg gccagcaatt attacgatta cactgtctat gaatcgttcg cggcgggagc
    18841 ccgcgcgcgc agcatcttcc ccctcttcgg cgccgaaatc atcgcgatgg atgacatcct
    18901 gcggctgtcg ggcgtcaaag tgaatgaccc cggcaatccg ggcaaaatct atttctgcgg
    18961 caagggaatt gtccgcttcg atcccgccga tcagcccccc gcggcgcaat acctgctgga
    19021 gcagatccgc cggcgcgatg cgcaccggat tgatgagatg gcgcgccggc tggcggacta
    19081 ttgcgccgca cacggcgtag agtcgcccct cgatgcggcg gatatccacg cccgcgtggt
    19141 cgcgcgccat gcctgcccgc cggcgacggt gacgctgcag gaacgccacc tggcgcaggc
    19201 ctatcaggag ttgctgttcg agacgacgcc gcgcgaccgg atcaccgacc gcctgcgggc
    19261 cctcctgggc gcgccggtgg agccgtccga cgatccggcg gcggtgcaga acgcgctgcg
    19321 cgcgcacctg ctcaagtccg gcaagccggc cttcgtggag gaagactacg tcacgctcga
    19381 ggaagcgcgc cggctcgtgc tcgagctggg cggcatcccg tgctatccgg ttctggccga
    19441 tgggaccgag ccgatcaccg agttcgagag cgatcccgag acgctcatcg cgcacctgcg
    19501 gcagcttcgg gtccactgcg tggaattcat tccggtccgc aaccgccccg aggtgctgct
    19561 gcgctacgtg cgggcgctgc gcgcggccgg gttcgtcgcc acggcgggaa ccgaacacaa
    19621 cacgctggaa ccgttgccgg tcgcgccgac gtgccgcgac ggtgcgccgc tgccggctga
    19681 agcgcgcgcc atcttctggg agggggcctg cgtcgtcgcc gcgcatcaat tccacgccgc
    19741 gcgtggcgaa gacggattcg tcaatgcacg tgacgagccc aaccggcgct ttgaaaacga
    19801 cgaacagcgc atcgccgcat tcgcaggaac cggagcccgg ctgatcgaag ctttttgccg
    19861 gagagcctga tggcccgcgc cggccgagac cttgccggct tcaccagcag gccagtcccg
    19921 ccagggacga cagacttcag cccgccacgt cagtggcggg aaactggaag gaaatagcga
    19981 aaagtcccgc agggacgaca gaacgtttcg catccttcct gtgccgggca ggaccgaaag
    20041 cgaaggccat acgatgaaga gacggaaatc aaacgatgac ggacgctgat cgccagaccc
    20101 cgcaggagaa tcgcgcccac acggacgcct ttggcgacat ggcgcgcgtc gaaccggagc
    20161 gcgcccgccg gctcatcgac cggctcggtc cggcgttgcg ggccctactg ggcgatgcga
    20221 cggcgcgggc ggtcgtgtct ttcgacgatt ccgacgaagc gcggcaacgg gtgcggatcg
    20281 tcccgaaccc cggcatgacc gcgtccgcgc caacgatccc cggccaaacc atggatgacg
    20341 ggtcgcatcc tttgtggttg gcagcacctg aggagggcga ggacgacgag gcgctcgtcg
    20401 gccgcctgcg ccaggcgatt gcggcccaca tggtgcgcta cggcgccgcg ccgcgcgccg
    20461 tgatcatccc gggcctgggt cggttcgccg cccgccgcga aagccagccg gcggcggacc
    20521 gcccggcggg tcgcctggcg ggcaaggtgg ccgtcgtcac cggcgcggcg caaggcttcg
    20581 ggcgcgaaat cgccgccggt ctggcccgcg aaggcgccct cgtggcactg gcggacgtca
    20641 accaggcggg cgtgagcgcg gcggcggcga gcctcgccga cgaagtcggc gccggccgcg
    20701 cgcatggcct ggcgatggat gtgggcgacg cgcgctcgat cgccgaagcg ctgcatcgcg
    20761 tcatccggtt gtggggcgga ttcgacatct tcatcgccaa tgcgggcgtg ctgcgcgccg
    20821 gcggcgtgca ggatctgacc gaggccgaat tcgatctggt gacgcgcgtg aattataagg
    20881 gctatttcct ctgcgtgcgg ctggcggcgc gcgtcttcgc gctgcagcac cgggcgttgc
    20941 ccggacccta cggcgacatc attcagatca attcgaaatc gggcttgacc ggatcgaacc
    21001 ggaattttgc ttatgccggc gggaaattcg gcggcatcgg cctgacccag tcgtttgcat
    21061 tggaattaat cgccgaccgg gtgaaggtca acgcgatctg tcccggcaat ttctttgatg
    21121 gacctctctg gtcggatccg gaaaatggat tgttcgtgca gtatctgcgc gccggcaagg
    21181 tgccgggggc ccggacggtc gacgacgtcc gccgcgccta cgaggcaaag atcccgatgg
    21241 gacgcggctg caccgccgcc gacgtgctga aggcgatcct ctacgtgatc gaccaggact
    21301 acgaaaccgg ccaggccatc cccgtcacgg gggggcaggt gatgctgagt tagaaccaat
    21361 atgggaccgg tggaaaggta ggacgtgaga gactttacgc ctccgactgc ggaggaaatt
    21421 gaccaacact ttgagaaatc tagacacttt actccagacc agttgcttga cgtcatggaa
    21481 gaaatggacg aggcagcttt tcgcaatatg aacgccgacg agattgagta atccccctct
    21541 gaaggggatt gccctgcctc aaaataggaa gcaacaaaat gaggactgat cgctaaatgg
    21601 cagttccacc atttcaagac tttatgttgc acatcctcca gttcgcgggc gacgctaagg
    21661 agcataccat ggcggaagcg cgcgagcatg ttgcgaagga attcggactg acgcaggagg
    21721 atcgagagga attgctgcct agcggccggg ttcctcgctt cacaaacagg gtcgcatggt
    21781 gtcgcgtcca tcttcagaaa gcagggcttc tggagtccag gcacagagga agtttcaata
    21841 ttactccact cggcctcgac gtcctgaaga agcaacccaa gcggattgat ctaaagtatc
    21901 tcgatcagtt ccccggtcat ttagagttct acaagcgagc gaagaaaaat gatggggaga
    21961 taaacgaagg acagcctcgc cccccggatg aagtccttga agagagctat caggaattac
    22021 gttctgaact agtgaccgca ttgctctctg cgttggaggc agccagccca tccttcttcg
    22081 aacgggtcgt tgtggagctt ctggtgagga tgggttacgg ggggtcgtta cgggatgcgg
    22141 gacaggcgat cggaaagagc ggcgatgaag ggattgatgg gatcataaag gaagatcggt
    22201 tgggattgga caatgtctac atccaggcaa agaagtggac tgctgttgtt gggagaccgg
    22261 agattcagaa gttcgtgggg gcccttcacg gcaagcacgc gaaaaagggc gtcttcatca
    22321 caaccagcag cttttcgagc gaggcgcttg agtacacgcg cctgattgat tcgagagttg
    22381 tcttgataga tggggtgact ctggctggtt atatgattga ttttggcgta ggcaccaagc
    22441 ttaaacaagt gtacgaacta aaggaaatcg acaacgacta ttttgatgaa gaatagcaaa
    22501 ttaaaaggaa atacaaagcc aatgaccacc accgccatcc cgcaaaagca atacgccgtt
    22561 cagctcgtcg ggccaagcca actcaaactg aatcccgaca aggatgtgcc gcggccgggg
    22621 ccgcaccagg tgctggcgaa gatcgaggcc gtcggcctct gcttctccga cctgaagctg
    22681 ctcaagcagt tttccgaaca cgcccgcaag aaggaagtcg tcaagggcct gagcgagcag
    22741 atcctcaatg gcatcccgag ctacgtgccg gccgacaagc cgaccgtccc aggccacgag
    22801 gtcgtcctgc gcatcgtggc tgtcggcgac aacgtgaaac accacaaggt cggcgagcgc
    22861 tgcatcgtcc aggccgatta ccgcaacctg cgcacctcgg gctccaatgc cgcgttcggc
    22921 tacaatttcg aaggcggcct gcaggaatac gtcctcatgg atgaacgcgt cgtcgtcgat
    22981 gaagacggcg agcgcttcct gatccccgtc aaggacgaca tcggcgcctc gcaagtcgcc
    23041 ctggtcgagc cctgggcctg cgtcgaggac tcctacgtga cgcgcgagcg gcaatcgatc
    23101 ctcgccgccg gccgcctgct cgtcgtggcc gacgccggcc atgccatcga agggttgcgg
    23161 gaatcgttct cccccgacgg cccccccgcc tcaatcacga tcgtctgtgt ggaagacgtc
    23221 cagctcaagg cgattcagga cctgggcctt ccggtcgagg aggccatcga cccggtggcg
    23281 ctccccgacg agggcttcga cgacatcgtc tacttcggcg cctccaaacc gacgctcgac
    23341 gtcctcaacg acaagctcgc ggcccacgcc atcatcaacg tcgtcaaggg gggcaagcgc
    23401 atcggcgagc cagtcgcggt cggcgtcggc cgcgtccact acggcccgac gcgctggatc
    23461 ggcaccgact ccacgcgcgc cgacgacgcc taccggaaca tcccgcccga tggcgacatc
    23521 cgcgatggcg actcggtcct cgtcgtcggc gcgggggggc cgatgggtca gatgcacgtc
    23581 atccgcaatg tctgcgccgg gcgcaaaggc gtctcggtca ccggcagcga tctggacgcc
    23641 gcgcgcctgg ccgcgctcga ggccaaggcc cgaccgatgg ccgccgccaa caacgtctcg
    23701 ctgcggatgg tgaacagcaa ggaagaaaaa ctggaccagg cgttcagcta ctacgcgctg
    23761 atggcgccgg tcgggcagct tgtcgccgac gcgatccgcg acggcgccga ggggatgatc
    23821 atcaatatct tcgccggcat ccccgccccc gtgaagcacg acctcgacct ggacacctat
    23881 ttcgccaagc gcgccttcat gttcggcacg agcggctcga cgatccgcga catgaagatc
    23941 gtcctggaga aggtcgagtc gggacagctc aacaccaatg cctcggttga tgcgatcgcc
    24001 ggcatgtccg gcgcgacaga cggcatcgcc gcggtggaaa accgcacgat ggccggcaag
    24061 atcattgtct acccgcaatt gcacgatgtt cccttgattc cgctgagcga attgggcgag
    24121 cattttccca cggtggccgc caaactcgac gacggccagt ggacgcccgc cgccgaggcg
    24181 gaactcctgc gcgtggcgaa ggcgtgagcc ctggggtgcc ctccatccgc tccatccgat
    24241 ccgtcgggtc cgccggatcc gaccgattct gctcgtgaac acgtgagatg caaaacgcgg
    24301 gacagacgac ggaaaacccg atggccgccc ctccccccaa gcccgccgtc gatctgctcg
    24361 cccgcgcgcg ccgcgtgatc gaactggaag ccgaggcagt cgccgggctg accgccgtcc
    24421 tcgatgcctc cttcgaacag gcgatcgccc gcatgctcgc cgtgcgtggc cgcgtcgtca
    24481 cgaccggcat gggcaaggcc ggcctgatcg cccgcaaggt cgccgcgacg ctcgcctcca
    24541 ccggcacgcc ggcgctcttc gtccaccccg ccgaggccgt ccatggcgac ctcggcatga
    24601 tcacggccga cgatctggcg ctggcgtttt cgcaccgcgg ccagaccgag gagatcctcc
    24661 gcatcctccc ctacctcaag catgtcggca cgccgctgat cgcgatcacc tccaatcccg
    24721 attcggaact ggcccgccat gccgacgtgg cgatgatcct cccgatccgc caggaagcct
    24781 gcccgctcaa cctcgcgccc accgccagca cgacggcgat gctcgcgctg ggcgacacgc
    24841 tcgcgctcgt cctgctcgag gcgcgcggct tccggcccga ggattacgcc gtcttccacc
    24901 ccggcggttc gctcggccgc cgcctgctga cgaaggtcgg cgacctgatg cacaccggcg
    24961 aggcgaaccc cgtcgcccac gaggcgacgc cgctgcgcga ggccctgacg gtcatgaccg
    25021 agaagcgctt ggccgcgctc agcctgaccg acgacgaggg caggctcacg ggcttctaca
    25081 ccagcggcga tctctcgcgc cacatcggcg acgccggctt cgatgtcaag cgcccgatcc
    25141 gcgacttcat gacccgcaac cccaagcacg cccgccccga gatgatggcc gtcaaggccc
    25201 tcgaaatcct ccagcaatac aagatcatcc agcttccagt ctgcgacgac gccggccgcc
    25261 cggtcggcat gatccatctg cacgacatca cccgcgcggg gatcacgtga acgccgcgcg
    25321 tctgctgatc gccaccggca accgccacaa ggtcgacgag atcgccgcga tcctgcgcga
    25381 cctgttgccg gcgttcacct gggagctgcg ctccgcggcg gatttccctc acgttcccga
    25441 accggtcgaa gacggcgaca cgttcgaggc caacgccgcg atcaaggcgc gcggctgggc
    25501 gatgcacccc gacgtgcgcg ccgtcggcct ggccgccctc gccgacgatt ccggtctggt
    25561 cgtcgatgcg ctcgctggcc ggcccggcat cctcagcgcg cgctacggcg cgacgccgac
    25621 ggcccggatt gaacgggtcc tgcgcgagat gcgcgacgtc ggcgactcac accggtcggc
    25681 gcgcttcgaa tgcgtcgtca cgctggcgag tgcggatggc gacctctgga cggcgcgcgg
    25741 cacggtggag gggcgcatca cgcacgcgcc gcgcggcatg gcgggcttcg gttacgatcc
    25801 gatctttgaa ctgaccgacg ggccgcacgc cgggctgacg ctggccgaag tccccgagtc
    25861 ggttaagaat ggcctcagcc atcgcgcccg cgcgctgcgc gccctcgcgc cgacgctggc
    25921 cgccatgctg cgcccggccg gcgtttgacc ggctccacgg ctcgacgcgc acccgcgccg
    25981 gccgccggct cacatcagcg ggctgatgaa ccagttccag atatccagcc cggaaatcgc
    26041 gaccatcagc gtgataaaga agatcacaaa caccgtgtag atcggcgcca ggattttcgt
    26101 cggcaccggc cgccggatca ccgcctcgat cagcgacaac acgatgaacc cgccatccag
    26161 caccggcagt ggcagcagat tgaagaacat cagcagcagg ttcagcatga tgaaccagtc
    26221 gagcagttcg ctgatgcccc ccatcgcgcg ctggaatgtc atcacggcga ttgcgatcgg
    26281 cccgcccagg ttctgactga tgtccttcgc ggtggcccgc ttgaaaaact ggacattcag
    26341 attcagcatc cccaggaacg cctgcatcgt gctgacgggc gccagcgtga tcgcgcggaa
    26401 cggcgtctcg cgcagaacac gcgtatcggg cgcgccgttg aacacgccga tctggccggt
    26461 cggtttgtcc ttcagcacat cgcggcgggg gatcacggtc agttcaagct gttcgccctc
    26521 gcgctccacg gtgatccggg tttccacgcc gggacgcgcc gcgatcagcc gcgtcatgtc
    26581 gctgaacgat tcgatcggct ccccgtcgat cgcgacgatc cggtcccctc gcttgatccc
    26641 ggcccggtcg gccgggtagt tcgggatcaa ctcgccgacg atcggcggaa tccgcatgtt
    26701 cagcgtgact tcgtccagca gccggtccag ttgcttgttg gtgatcccct cgagcgccag
    26761 ccggcggttc tcgccgccgc gctccaccgt cagctccatc gccgtcagcg cggtcgtcgt
    26821 cggctcgtcc ggcggctcca catagggcag gacgccggca agaaaacgat ggtaaatctc
    26881 gccgacgccc atcggcccgc cggttttctc gatcaccgcc tgctgcaagt ccagactgta
    26941 gggcgtcggc gcgccgtcga acgcgacgat gcggtcgccg ggctccacgc cgatctcggc
    27001 cagcaggctc gcctcgggca cctgctccag ttcgatcgga aaaagctgcg ccttggtgcc
    27061 gacgagcccc agcagcgcgg cggcgacgat cgccgtcacg aaattcatga agaccccgcc
    27121 gccgaaaacc agcagcttgg tcaccagtcc ctcgtcgcgc agcgcgagca ggtcgtcgta
    27181 ggccgattcg cttaattcct tgtcttcgtg gtggccggaa gcaagatccc ccgcggccga
    27241 ctccgactcg gcgacggccg gctcctccag ttcttcgggc atcatcccct tcagcttgac
    27301 gaacccgccg gcggggatcc agcgcagcga aaactcgatc ccgcgccact tccacgacgt
    27361 gattttgggg cccatcccga tcgcgaacga ctcaacgccg acgccgcgga tcagggcgaa
    27421 aaacatgtgg ccgaattcgt gtacaaagac cgccagcccg aagaccagca ggatcgccag
    27481 cgcgcccagt agaatatcga gaatcgcggt aaaggacatc gtcacactcg gaccggggga
    27541 tcgggttcgt ctgtcccggc cgccgggccg ggccaaacgt ttcggaatcc gagtttacca
    27601 ttgcggcatg cgtctgagaa caagaatggc ggcgtcccaa atgcgcggac cacctttcaa
    27661 accggaagtc gcccctcgtc gccacgaaaa aatgcgccct tcgtccctcg tccttggctc
    27721 tcgaaaaccc gctgttctgt ccttccgttg tgaaacgcgc cagctgagcc cgagggatc
//
LOCUS       DAOPCO010000027        26471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2005_length_26471_cov_5.095889, whole genome shotgun sequence.
ACCESSION   DAOPCO010000027 DAOPCO010000000
VERSION     DAOPCO010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 26471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26471
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2005_length_26471_cov_5.095889"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1231
                     /locus_tag="PLS90_03750"
     CDS             179..1231
                     /locus_tag="PLS90_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01550.1"
                     /translation="MAKKVRVAFIGAGELANIVHYPSLASFKDVEIVALCDLSEERRN
                     ATAAKFNVARTFSDYRQMLQSVKVDAVYVILPPQLLHEPVVYCLKAGLHVFTDKPPGV
                     TPHQTRAWAGLAARHKNLTMVGFQRRYAPIMRKAREQVIKRGGAIQHAVAQYYKGYKG
                     GLYYEGAIDILTCDAIHAVDALRWVAGGEVVDVTSDLRDQVGRGFHDLFTATVRFSNG
                     CLGFLFGNWNCGAYRFAVEMHANRTSAFVEINKRALYHEDGNQEGEVWETRDVAGSDD
                     MLDYAGFRAENRHFIDCIKSGRQPDSHFGDAAKSMELVEKIHRAARPEPSSVPAPPPQ
                     KASGAGRSRPRRAKSR"
     gene            1523..2680
                     /locus_tag="PLS90_03755"
     CDS             1523..2680
                     /locus_tag="PLS90_03755"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01551.1"
                     /translation="MNGKLGIGFVGVGWMGSVQLKRLLERDDVEVRHVCQPDKPGALR
                     VLDELGLPHSLYTPDYDQVVADKGIDCVWLVSPNRFHGPQSIQALQAGKHVFCEKPCA
                     TKYDEYCTQLELARSNPRLMTFVDYILYFDAMEQNLREMVARDEFGAITQIQVNYRHP
                     INIAGDKAWKLSREVMGDAMGMGIIHSLSVIYWIMQSQARPVSVFASAMPAQVRPFEA
                     LSIWNIQIHFDNGATGFCFGNIDTSKGYDAYHNLSGTKGGFIFDSQTDHSHKVRLWSE
                     KTTDGRWIYPLDAARCEREGVGQLAWPADTSTPDSGDVIHHQTAEAVGHFLECVKSGR
                     QSPLSFVNAAPITELGWAALVSAELKRPVELPLEPAAAREILARADSAASA"
     gene            2741..3724
                     /locus_tag="PLS90_03760"
     CDS             2741..3724
                     /locus_tag="PLS90_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01552.1"
                     /translation="MTAKLGLVGLCTSHPQNWVPIIRALADEKKVDVEVVAAWDSGET
                     RHEGWSREFCEEFHIPHVIEKLEDMVSGVDAVIVHTANWDRHVEQARPFVEAGKAVLL
                     DKPVVGNLRDARQLIDWSRQGARLTGGSSLRFAPEVREYLARPVDERGTVHTAYAGCG
                     TDEFNYGIHAYALLAGLMGPGVRSVRYLGASAQKSLQITWNDGRIGLLTIGKGAWLPF
                     HITAVTDKNVEQIQVDSKRIYRALLEATLPFLTGAEAKPPLTIEELLEPELTALAARV
                     SWQSQGAQIFLDDLRLDDPGYDGHRFGSEYRTALAARRAEMLREKLKEKAS"
     gene            3721..5127
                     /locus_tag="PLS90_03765"
     CDS             3721..5127
                     /locus_tag="PLS90_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01553.1"
                     /translation="MKPTDVRLIGAELYYLPLKLRVPLKFGAETLTHLTCARARVTIR
                     DRAGKTARGWGETPLSVAWVWPGQDPFADREEQLMRLCERIARAWSGFEAEGHPIEIG
                     HDFQEQTLPGLLRQANDERPGHLTAIPWLAALVACSPFDIALHDAYGNLLGRPVYDTY
                     TAEFMNRDLAGYLEPIEDGDVSFAGRYPADFLEAEPPAALPVWHLVGGLDPVDRAALT
                     GNEPDDGEPVLLEDWIRRDGLKCLKIKLRGNDAAWDYNRIVEVGTLAARLDAHWLSPD
                     FNCTVKSPEYVNQILDRLLREQPRIYGMLLYVEQPFPYELESHPINVHSVSARKPLFM
                     DESAHDWKLVRLGHALGWTGVALKTCKTQTGALLTLCWAKAHGMTLMVQDLTNPMLAQ
                     IPHVLLAAHAGTIMGVECNAMQFYPRESDLEARVHPGLYQRRNGRLDLTSIRGSGFGY
                     RVDEVSRELPPAVVEHAP"
     gene            5124..6134
                     /locus_tag="PLS90_03770"
     CDS             5124..6134
                     /locus_tag="PLS90_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPK01554.1"
                     /translation="MKQPAEDLLPAVRAAARQWGLHDASVRPLGGGTANRNFLLSSPA
                     GRHVLRLRNPRYAAPEALAYYHGLMQHLHRAGLQAPYPLPTVDGKLSLVTELGTLELY
                     PCLDGEPFDETQPAHRMHAAEALRRFHQATASFDHPYAHRQPRIDSPEMIRQGLEEIR
                     AQTTDPEQLRILEQLFQWHRRLVEALPDERFWALPCQVIHGDFHPANVTISPTGLNLF
                     DFDNSSWQPRLRDLADAMLFFCGRREGRFDASTIVTLTKETTLDPARHDAFLQAYGPL
                     TSEEAEALRWVVVARWIYCRVDGRRKLPPAAWPAFVTNGVLPLLGQIEEGLLRPQKSN
                     AR"
     gene            6147..7028
                     /locus_tag="PLS90_03775"
     CDS             6147..7028
                     /locus_tag="PLS90_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK01555.1"
                     /translation="MNSASDSIPASALVLGASGMIGLPLALALAERGARVYGAARFSD
                     AAKRRMLEERGVAIVPFDVLRDDPAALPDVAALFLEIWDPANPKLTFELNVHAVGRVV
                     ERYAGAATIVNGGTINVYGAGDYLPREDSPCQPDSPYGLSRFVQEQMIEFFCRQRGGK
                     AIHVRYAHTNSASRGMIHWMASRILGGESLGPHPDRRIQVIALEDAVRVTAAACSRAE
                     HPTAAVNCCHPREWGWRELAEEIHRRLGRGSVVFDAPAGGVATTVCADAGRMVEWFGP
                     PQVTLEAMIERVVRELS"
     gene            complement(7227..8906)
                     /locus_tag="PLS90_03780"
     CDS             complement(7227..8906)
                     /locus_tag="PLS90_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPK01556.1"
                     /translation="MPNDLPPDPDPTAPPPPLSRNPVSYLGWIVVAITGAALVVLILM
                     DILQHQENPYNSLVTYLVLPGVLGAGIALILGGIALEWRRRHRKAPSTYPRLPLIDLN
                     LPWQRRRILAGVGIASLLFTVSSVGTYQAYHFTESPVFCGQICHHVMEPEYTAYQHSP
                     HARVACTQCHIGPGADWYVRSKMSGLRQVFAVLTKSYELPIETPVHNLRPARHTCEQC
                     HWPAQFSDSVEKIIWHFSPDRANTPARYNLLLKIGGGMPDAGLGRGIHWHINPSIEVR
                     YWARDRQRLDIPYVEVRREDGSTESFRSPDCPDPLPPDAELRLMDCIDCHNRPSHIYR
                     SPKQLIDFYMATGVLDDSLPYLKRYATEIFEQPWPDTPSALAAIDGLRERYADRVQGP
                     EAARVLETNIDWLARLYQRNFFPEQGVDWRAYPNHLGHFEFSGCYRCHDDRHVSDRAP
                     AAVREAVARAAQGTGREAEPNGTARAAAGEAAPATRAGHVISNDCRLCHEILDQAEGA
                     AAFAPADYTGGGEFNHPRGLGDIWLGNNCTDCHGLTGQPQPPRQLTQATPR"
     gene            9055..10086
                     /locus_tag="PLS90_03785"
     CDS             9055..10086
                     /locus_tag="PLS90_03785"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01557.1"
                     /translation="MALFAVWGLAAGEAAGSDMPHAGQLDSHFDQNCSTADCHPHAPA
                     ADAVPHPPYLENNCLACHEDHASSATALLKPDVEQVCLACHTQVHPEARPAADHPPGS
                     SDCLRCHQPHQSPTRGLLRDAEVLGICADCHRPFLEEAATLPYRHRHFDLQGQCGNCH
                     YAHREAEASYLRAEVTETCLTCHDLPIQSGGRTLENVARELREQPVVHAAIDEGCPTC
                     HTPHGSVQPALLHEGYPAGNYGAYEEENYRLCWRCHDAGLVEQADGRGATEFRDGAKN
                     LHRVHVANLKRGRTCHLCHEAHAADRPHLLRRTMKFGAWEGPLEWRETEGGGSCQTPC
                     HREAGYGRQ"
     gene            10196..11860
                     /locus_tag="PLS90_03790"
     CDS             10196..11860
                     /locus_tag="PLS90_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPK01558.1"
                     /translation="MAAKVQHHRPPEPGLLDWLSSLKLGLVLLTLVGIASIAATLIDP
                     LPKAQQLVYFTWWYKVLLLLLAVNIACATWRTVTRRVLPLRRLRPMRQKEYYRTAPLR
                     TDIAFHGTAEAVAQAFGRRRFRVAREGEFGHARRGYLSRWGAPVSHVGFVMVLAGGFL
                     SPFFMIDGYVRLAEGQATDTVHLRGEKGGTLALDYQVRVEDFDLTYFPQTEMPASFVS
                     TVSILEGGRTVKTGPVEVNRSLKHGGLKFHQTGYGEQPSLARHAVELTHPRQAPAGIQ
                     FELSPGQVRAVPGLPGYEIALHAHAPPHWVLSDATGPVAEGAIRGVSGPVTIRAERFE
                     PDFVIGPNRQVGSRSAELRNPALEVSLIEGETAAIRRWLFGKPEMRAMSHGGGGPFDL
                     ELAEVRGEGESRRFVLRALAPDTGVEVDRAELALGESATLGEQAAGEIPVEDATDWRL
                     EIVGRAPAYTTDLSVTRNPLIPFIYLGCGLMLLGLVLAFFVKQRDVWFHVDAARGRLW
                     VVAAYRHPHGELDRVVRSVVEGLREPEAIPGGAPASEPEVARAATA"
     gene            11908..13515
                     /gene="ccsB"
                     /locus_tag="PLS90_03795"
     CDS             11908..13515
                     /gene="ccsB"
                     /locus_tag="PLS90_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPK01559.1"
                     /translation="MSEACRRWRDIDTSAGSGLRPSYRRVIWIAAWLAFVAAMWTVPA
                     RGAESAAPAMSPAWEAALRPLERLPVQDTGYVRSAHTYARTRLRGITGRNGLGELNAV
                     QSLIWLVSHPAAAAREPLIRVTHPELGSLLGGRRTSLDTYRNGRVRERIVAALRADEN
                     LLKPINELETRAAMFEHLEAEMAFVPRAGEWLSPVDAAGRDDLSSEERELVELWAALK
                     RAVAEDNPTAGGELARELAARAPRLAEARGLALPRLEADWFYHTHEPFRKSSLFYLVG
                     ALGFGAAFLLGRRWVYWSSLALMGGGLAEQVLGVGLRWIVAGRAPLSNMYESFTFATA
                     GMILLALVFEWIYRTRLAGLGGTILGFAFMVIAHNVAIFDSQIRPLMPALQSSWLTYH
                     VVTIMLSYSAFALSFFVALVYLAKEALGGDGSVRLALRKLPSLQALDLFNYRIIAVGF
                     PLLTLGIVTGAIWAATAWGRPWAFDPKETWSAITWLIYAIYLHVRYMAGWRGRRAAVL
                     ALVGFAAVLFTYLGVNYLLPGLHSYAA"
     gene            13681..14910
                     /gene="hemA"
                     /locus_tag="PLS90_03800"
     CDS             13681..14910
                     /gene="hemA"
                     /locus_tag="PLS90_03800"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPK01560.1"
                     /translation="MTAAPQDSQPTLHLCGIHHSTSPVAIRERYALAPDQVGAWMRRL
                     REETGAQVLIVSTCNRMEIYAWGSGALGNELRAAMRRIGLAAGCAEAPELIERSGEEA
                     LRHLFRVVAGLDSMILGENQIKQQVREATEMSRAAGCLGSELNQAVEAAFRCGKRIRT
                     ETELNVGTLDVGKAAILKAEERLGGLTGRACMVIGAGKIGRTAARAIAERRPDRLWIV
                     NRSLEHARAIAEELGGEAYGLEALGCLLPEAEFILGAAHTPELLFDAEDYEVAAPTGS
                     RPERVCLVDVAVPRMLDPRLGELAGVELYGLEDMEEITAVNRRQREAAAEAAGVIVEE
                     ELRQWRRERAGGALGPAIEELKARLETIFAEEEAALAEALGEAPADRLTPALRKLRGR
                     TLHAAIESLKRAAGGDC"
     gene            complement(15120..16676)
                     /locus_tag="PLS90_03805"
     CDS             complement(15120..16676)
                     /locus_tag="PLS90_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPK01561.1"
                     /translation="MRRTAGLDSPISIPMLVVLLLTLVATAQAELTLPQLFGDNMVLQ
                     REAPIPVWGWASPGQEVEVTLGDNSAAAVAGADGRWRVELPPMEAGGPHQVTITAGEQ
                     SRALENVLIGEVWLCAGQSNMQWSILVVRDAEQEIQAANYPRLRLFNVPVRSSGVPAD
                     DVAATWRVCSPATIAQDGGFPAVPYFFGRKLHQDLDVPVAVIAAAWGGTRIEGWIPPE
                     GYASNPALADYLLQIEQAGPKFRDDLSTAVPQYESWLPEARRALDAGERVPDPPLWPR
                     HPLDSAERPTGIYNGMIHPLVPFALRGAIWYQGESNAGDGMMYFEKMKALIAGWRQLW
                     GRPELPFYYVQIAPCDLDPLYAGDALPELWEAQTAVMRAVPHTGMAVISDVGDLHDIH
                     PRNKQEVGRRLALWALAETYGRDDIVPTGPIYRSLAVEANRLRIEFDYVGQGLSTRDG
                     KDPDLFEVAGADGQFVPARADIVGDAVVVWSDDITTPTAVRFAWSRTAVPNLMNQNGL
                     PASPFRARVE"
     gene            17079..17726
                     /locus_tag="PLS90_03810"
     CDS             17079..17726
                     /locus_tag="PLS90_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK01562.1"
                     /translation="MRRLHRYAAIAASLLLTAIWIAPAQASLIGDRIDYSFQDDNGFS
                     QSGEDLLVRSGSEISLTAPLYRLDVNLGSDWVQINQNSRSAADDKYRRTGKCTFPGWE
                     LLLADLDLGEDREITGYRLTASGIDRNLIGVNFTADSLTVEFAGLTYGSGKNSKSLWN
                     DLLKCYDPCKLDFCLKIDLITSPKPPVIPEPATLGLVTLGVGGVGLLRRRVKKLK"
     gene            18057..18344
                     /locus_tag="PLS90_03815"
     CDS             18057..18344
                     /locus_tag="PLS90_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014733561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPK01563.1"
                     /translation="MQDGPRTSLQARIQGRVQGVGFRFFVLREARGLGLTGTVANRPD
                     GSVEVKAAGGREALEELLGRLRRGPTLSRVDHVEVQWDIPLPDMSEFTIEF"
     gene            18349..19152
                     /locus_tag="PLS90_03820"
     CDS             18349..19152
                     /locus_tag="PLS90_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01564.1"
                     /translation="MLLYTDLVRDIARDVARRVPDLAHIDADRIAFLAAARCTSHRTG
                     NLAICYGLNRQAEPTFSVWVRRGTRKVIAVSGWVQMRVPRVYINGREMSYLILLRLPR
                     LLRQNPLETIIHELYHISEGFDRRMRPARHGALFDREVRRLMNVWLANARGDLPRLAQ
                     MTLRQLEGEFGAVLAQGVPSNFRLPIVEPVRGPESYQRAVPRLYPGHSLAALYQVRPA
                     QQLSPEETPRVLTERDLVLRHYHTRGVERVPAEFTRYSRNGRRSLALSA"
     gene            19165..20115
                     /locus_tag="PLS90_03825"
     CDS             19165..20115
                     /locus_tag="PLS90_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK01565.1"
                     /translation="MRSASPRPPGFVHVDLDGLWTLAGVYGFPEQDLFELDPVFNLAV
                     DRLLELLGSRGIRATFFIVGRDLELPEKVDAVARIAAAGHRLANHSLRHDLRLGEAAP
                     GSIEEELRTVNERLSAVAGEAPLGFRAPGYAVNERVLTACVRAGLRYDGSVFPCWWAP
                     LLRRLAGSLRQSPGPPARGQYGGAGSLRPRWERPVAGAPPILRLPVAVSPRLRLPLHA
                     SLGLLLGAGTVRRGLRNLAARGLPAVYLLHGLDLLGAEEFAGRLPGPLAGHRAFRPSL
                     ASKREFLDTVLRAFGELFAIETTEDWMRRLEVGRSGGISA"
     gene            20416..22137
                     /locus_tag="PLS90_03830"
     CDS             20416..22137
                     /locus_tag="PLS90_03830"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HPK01566.1"
                     /translation="MAGKRSATGRAGRSAPAGGGTLQEFRGLVEEYYEQTFERFPIEG
                     SRAGRRECDGELGRARPGDFERQGRLVERILAAVEDLPPQDFDASAMLDRRTFVAALR
                     LEHLNLVELERWRNDPQRHLHAVAESLLELVVRNADRLGAVAPGILSSLRQAPRYFGD
                     AAECLDRPDPLWRELTLEAIPGVISFFNSLAEPLIQTQAAPAGVIRRTVAGACAAVEA
                     YGDAVRSTRPGKAGSFAVGERRLRLLMRDRLGLDWSPREAMAFARREATRIAAELRRE
                     AAHFHPRKSPVEILAEAAAAWRPDSGLLEAYTRVTRAVRERFEEAELVSFPKAERLLI
                     KPVPDFLRELFPTAAYTPPGAVDPDQTGIFWVNDPGDVDLPPARRAAEIAQHFGLEMT
                     CAHEAYPGHHLQFMVQHRRPSFVRKLANHAVYYEGWTLWCEQMTCDLLSQGVGRGRRA
                     RLRVPGAGLPENPHIRLNQLNDELWRAWRIVIDVGLQTGELTCENARRILQREVGFTA
                     ARARADINWYTSAPTIPMSYLIGKAELLRLKRRYVDSGGLTLREFNDWVLSFGAIPWR
                     WIEESGL"
     assembly_gap    22295..22394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22395..23419)
                     /locus_tag="PLS90_03835"
     CDS             complement(<22395..23419)
                     /locus_tag="PLS90_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01567.1"
                     /translation="MTRSTSVSDTDNNSRDGAAAAAAPAPGTGAFPDGPAIVTSNLTL
                     QVDRYEIFRDVSIQVSSGERVGLIGPIGSGKSALLKTLATLTRPTRGGGAVAGQDLLS
                     QADAIRPRVGYMPDVLGAYDDLRVWEYLDFFARAGRVEPRHIRSRIDRLLAEAELTDQ
                     REAFVETLGREEKRRLGLIKTLLHEPRLLILDSPAAGLPLAARRRLRELIDRLLAPDR
                     TLLLGTNILSDLVGLCSRAYVIAGGRIVLEGPMADVLRRLAPARAFEVRIGAETDSPE
                     RRARLAEQARETLRAAPGVDQAQSLADHVVFSTREPLEDPAPWLAALRAREIPVGGWR
                     EREIDLSV"
     gene            complement(23910..24914)
                     /gene="hprK"
                     /locus_tag="PLS90_03840"
     CDS             complement(23910..24914)
                     /gene="hprK"
                     /locus_tag="PLS90_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPK01568.1"
                     /translation="MVSPISELNEPLKVEELLSRKARDWDLVQVAGRDGLSNVIYTPE
                     INRPGLALAGYYEVFSAERIQLIGLTETRYLASLTPETRQQVIRQTFAFHIPCVIVTT
                     DLEIAEELITTCDALQIPLLRTSLTTSLFQADLSHFLERRLAPAWTVHGVMMDVFGMG
                     VLIQGRSGVGKSECGLELIERGHRLIADDVVVIRRISKNQLIAETPPNIGYHMEIRGI
                     GIIDVELLYGVRAVREESPINLRVMMEPWDPMKEYERLGLNDRESILFECSVAEYVLP
                     VEPGRNIAKLIEVAALTQRLEDQGINVAREFDRRIMEAIQKKQTTRRPFTAIDRLPRP
                     "
     gene            25287..25613
                     /locus_tag="PLS90_03845"
     CDS             25287..25613
                     /locus_tag="PLS90_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01569.1"
                     /translation="MQLARFNIGDSVRLRERIHPGHPYARLDQRSPNRSDIPAGTRGI
                     VRRSHGTTVMVELFPNPDIMTGRGIDVWVSYHMLVVLFAPDGTGAEGKDASMARKKST
                     GRLIHV"
     gene            complement(25715..>26471)
                     /locus_tag="PLS90_03850"
     CDS             complement(25715..>26471)
                     /locus_tag="PLS90_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK01570.1"
                     /translation="VGKTTLAYAVARAMDAEVYIYQCTMDTRPEDLLITPVIASDGRI
                     AYHASALVTAMIRGGVCILDEANRMSEKSWASLAPLLDQRRYVESIVTGLKIQASPEF
                     RICCTMNDDASTYEVPEYIQSRLQPQIEVTFPNREEELQILRYNLPFSGDELLEMAVS
                     FLQKAHRLNLRFTSRDGVNILRYCIKMGQLKGQDPRQFFDEAVQGVLGQDGTDFLNGI
                     VPPTARYHQRLRDQDELPDDGDDLEEGESGNPW"
BASE COUNT         4267 a         7968 c         9801 g         4335 t
ORIGIN      
        1 agctctgtcc gccctacgtc atcggggagg atgcgctggc ggagggtctg gatgtgctgg
       61 aaggcatcgc ggccgaactg agcgaatcgc tggttgtccg ttcctgaccc ggccgtaggg
      121 ccggttgaac cgctgcaagt cacgccgggg gttggctcgg cgaaggatga atcacaagat
      181 ggcaaaaaag gtgagggttg cgttcatcgg ggcgggcgag ttggccaaca ttgtgcacta
      241 cccgtcgctg gcgtcgttca aggatgtgga gatcgtcgcg ttgtgcgatc tgagcgagga
      301 acgccgcaac gcgacggccg ccaaattcaa tgtcgccagg acgttctccg actatcgcca
      361 gatgctgcag agcgtcaaag tcgatgccgt ctatgtcatc ctgccgccgc agttgctgca
      421 tgagccggtc gtctactgcc tgaaggcggg tctgcatgtc ttcaccgaca agccccccgg
      481 agtcacgccg catcagacgc gcgcgtgggc cggccttgcc gcccggcaca agaatctgac
      541 gatggtcggc ttccagcgcc gctacgcgcc gatcatgcgc aaggcgcgcg agcaggtgat
      601 caagcgcggc ggcgccatcc agcatgccgt cgcccagtat tacaagggct acaagggggg
      661 tctatattac gagggcgcca tcgacatcct gacctgcgat gcgatccatg cggtggatgc
      721 actgcgttgg gtggccggcg gcgaggtcgt cgatgtcacc agcgacctgc gcgatcaggt
      781 cggtcgcgga ttccacgatc tcttcaccgc gacggtgcgc ttttccaatg gctgcctcgg
      841 ttttctgttc ggcaattgga attgcggcgc gtatcgcttc gccgtggaga tgcacgccaa
      901 ccggacttcc gcgtttgtcg agatcaacaa gcgggcgctg tatcacgagg acggcaacca
      961 ggaaggggaa gtgtgggaaa cgcgggacgt ggcggggagc gacgacatgc tcgattacgc
     1021 cggcttccgg gcggagaacc gccacttcat cgactgcatc aagtcggggc gccagcccga
     1081 cagccacttc ggcgacgccg ccaagtcgat ggaactggtc gagaagatcc acagagccgc
     1141 gcgtcccgag ccgtcgtcgg tcccggcgcc gccgccacaa aaagcctccg gcgccggccg
     1201 gtcgcggccc cggcgggcga agtcccgctg atcggcggaa agaacgccgc gcggcatcgc
     1261 cgcaacgaac acgcagcctc gggccgaggc ccggtgcgct caaggctgcc gttcgttgcg
     1321 ttcttcacgt tcactgagtc catggcgtcc atcgggtcca ttgggtccat agccttcgcg
     1381 cttgaccttc cccagccgag cggctacctt gtggcgagta accgcagtgt gactaacgca
     1441 taacacgcat aacacccgcg acacccgcac ccctatctcg cgccgccgcg cgcaatccgg
     1501 cggcgccatc tgagaggctt ccatgaacgg caaactcggt atcggcttcg tcggcgtcgg
     1561 ctggatgggc tcggttcaac tcaagcgcct gctcgaacgc gacgatgtgg aagtgcgcca
     1621 cgtctgccag ccggacaagc ccggcgcgct gcgcgtcctc gatgagctcg ggcttcccca
     1681 ttcgctctac acgcccgatt acgaccaggt cgtcgccgac aagggtatcg actgcgtctg
     1741 gctcgtcagc cccaaccgct ttcacggccc gcagtcgatt caagcgctcc aggcgggcaa
     1801 gcatgtcttc tgcgaaaagc cgtgcgcgac gaagtacgac gagtattgca cccagctcga
     1861 actggcgcgg tcgaacccca ggctgatgac gttcgtcgat tacatcctct atttcgacgc
     1921 gatggagcag aatctgcgcg agatggtcgc gcgcgacgaa ttcggcgcca tcacccagat
     1981 ccaggtcaac tatcgccatc cgatcaacat cgccggcgac aaggcatgga agctcagtcg
     2041 cgaggtgatg ggcgacgcga tggggatggg catcatccat tcgctgtcgg tgatttactg
     2101 gatcatgcag agccaggcgc ggccggtgtc ggtcttcgcc tcggcgatgc cggcgcaggt
     2161 gcgcccgttc gaagcgctgt cgatctggaa tatccagatt catttcgaca acggcgccac
     2221 cggcttttgc ttcggcaaca tcgacacctc caagggctac gacgcctatc acaatctgtc
     2281 cggcacgaag ggcggcttca tcttcgactc gcagaccgat cattcgcaca aggttcggct
     2341 gtggtcggaa aaaacgaccg acggccgctg gatttatccg ctcgacgccg cccgctgcga
     2401 gcgtgagggc gtgggccaac tggcctggcc ggccgacacc tcgacgcccg acagcggcga
     2461 tgtgattcat catcagacgg ccgaagccgt ggggcatttt ctcgaatgcg tcaagtcggg
     2521 acggcagtcg ccgctcagct tcgtcaacgc cgcgccgatc accgagctgg gctgggcggc
     2581 gctcgtctcg gccgaattga agcgccccgt ggagctgccg ctcgagccgg ccgccgcgcg
     2641 cgagattctg gcgcgggccg actcggccgc ttccgcgtga tccgcatcga ccccagcgtg
     2701 gacaacgtat cgcatcccat ttctttcaca ggagctgaca atgacagcga aactcggtct
     2761 ggtgggcctt tgcacgtcac atccccagaa ctgggtgccg atcattcgcg cgctggcgga
     2821 tgaaaagaag gtcgatgtgg aagtcgtcgc ggcctgggat tcgggcgaaa cgcgccatga
     2881 gggctggagc cgtgaattct gcgaggaatt ccacatcccc cacgtcatcg agaagctgga
     2941 ggacatggtc tccggcgtcg atgccgtcat cgtgcacacc gccaactggg atcgccacgt
     3001 cgaacaggcg cggccgtttg tcgaggcggg caaggccgtc ctgctcgaca agccggtcgt
     3061 gggcaacctg cgcgatgcgc gccagctcat cgactggtcg cggcaggggg cgcgcctcac
     3121 cggcggctcg agcctgcgct tcgcccccga ggtgcgcgaa tacctggccc gtcccgtcga
     3181 tgagcgcggc acggtccaca ccgcctacgc cgggtgcggc accgatgagt tcaactacgg
     3241 catccatgcc tacgcgctgc tggccggcct gatgggcccc ggcgtccgct cggtgcgcta
     3301 cctgggagcg tcggcgcaga agagcctgca gatcacctgg aatgacgggc ggatcggcct
     3361 gctgacgatc ggcaaggggg cgtggctgcc gttccacatc accgccgtca ccgataagaa
     3421 cgtcgagcag attcaggtcg actccaagcg gatctaccgg gcgctgctcg aggcgacgct
     3481 ccccttcctc accggcgccg aggccaagcc tccgctgacg atcgaggagt tgcttgaacc
     3541 ggagttgacg gcgctggcgg cccgcgtttc gtggcagtcc cagggcgcgc agatcttcct
     3601 ggacgatctg cggctggacg accccggcta cgacggccat cgtttcggat cggagtaccg
     3661 cacggccctg gccgcgcgcc gggccgagat gctgcgcgaa aaactgaagg aaaaggcctc
     3721 gtgaaaccga ccgacgttcg cctgatcggc gccgagctgt attatctccc gctcaagctg
     3781 cgcgtcccgc tcaagttcgg cgccgagacg ctgacccatc tgacctgcgc ccgggcccgc
     3841 gtcacgatcc gcgatcgggc cggcaagacg gcgcgcggct ggggcgagac gcccctgagc
     3901 gtcgcctggg tctggcccgg tcaggacccg ttcgccgatc gcgaggagca gttgatgcgc
     3961 ctgtgcgagc ggattgcccg ggcgtggtcc ggcttcgagg ccgaggggca cccgatcgag
     4021 atcggccatg atttccagga gcagacgctg cccggcctgc tgcggcaagc caacgacgag
     4081 cgccccgggc atctgacggc catcccctgg ctggccgcac tcgtcgcctg ctcgcccttc
     4141 gacatcgccc tgcacgacgc ctatggcaac ctgctcggcc gaccggtcta cgacacctac
     4201 accgccgagt tcatgaaccg cgatctggcg ggctacctgg agccgatcga ggacggcgat
     4261 gtctcgttcg ccggacgcta tccggccgat tttctggagg ccgagccgcc cgccgcgctc
     4321 cccgtctggc atctggtcgg cggcctcgat ccggtcgacc gggccgcgct gaccggaaat
     4381 gaaccggacg acggcgagcc ggtgctcctg gaggactgga tccggcgcga cggcctcaag
     4441 tgcctcaaga tcaagctgcg cggcaatgat gcggcgtggg attacaaccg catcgtcgag
     4501 gtggggacgc tcgccgcccg gctggatgca cactggctgt ctccggattt caactgcacg
     4561 gtcaaatccc ccgaatatgt gaatcagatc ctcgaccgcc tgctccgcga gcagccgcgc
     4621 atctacggca tgctgctcta cgtcgagcag ccgttcccgt atgagctgga gagccacccg
     4681 atcaacgtgc acagcgtttc ggcgcgcaag ccgctgttca tggatgagag cgcgcacgac
     4741 tggaagctcg ttcggctcgg gcacgcgctg ggctggaccg gcgtcgccct caagacgtgc
     4801 aagacgcaaa ccggcgcgct gctcacgctg tgctgggcca aggcccacgg catgacgctg
     4861 atggttcagg acctgaccaa cccgatgctg gcgcagatcc cgcacgttct gctggcggcc
     4921 cacgccggca cgatcatggg cgtcgaatgc aacgcgatgc agttctatcc ccgcgaatcc
     4981 gatctggagg cgcgcgtgca ccccgggctg tatcagcggc gcaacggccg gctcgatctg
     5041 acctcgatcc gcggatcggg attcggctac cgggtcgacg aagtgagccg cgagctcccg
     5101 cccgcggtgg tggaacacgc gccatgaagc agccggccga ggacctgctg cccgccgtgc
     5161 gcgcagccgc gcgtcagtgg ggcctgcacg acgcctcggt ccggcccctc ggcggcggca
     5221 ccgccaatcg caatttcctg ctctcctcgc ccgccgggcg gcacgtgctg cgcctgcgca
     5281 acccgcgcta cgcggcgccc gaagcgctcg cctattacca tggcctgatg cagcacctcc
     5341 atcgcgccgg cctgcaggcg ccctacccgc tgccgacggt cgacggcaag ctcagcctcg
     5401 tcacggagct cggcacgctc gaactgtatc cctgccttga cggcgaaccg ttcgatgaga
     5461 cccagccggc gcatcggatg catgccgccg aggccctgcg ccggtttcat caggcgaccg
     5521 cgtcgttcga tcacccctat gcgcatcgcc agccgcgaat tgattccccg gagatgatcc
     5581 ggcagggcct ggaggaaatc cgtgcccaga cgaccgatcc ggagcagctc cgcatcctgg
     5641 agcagttgtt ccagtggcat cggcggctgg tggaggcgct gcccgacgaa cggttctggg
     5701 cgctcccctg ccaggtcatc cacggcgatt tccacccggc caacgtcacg atcagtccaa
     5761 ccggattgaa cctgttcgat ttcgacaact catcctggca gccgcggctg cgcgatctcg
     5821 ccgatgccat gctcttcttc tgcgggcggc gcgagggacg cttcgacgcc tcgacgatcg
     5881 tgacgctgac gaaggaaaca acgctcgacc cggcgcggca cgacgccttc ctgcaagcct
     5941 acggcccgct caccagcgag gaagcagagg cgctgcgctg ggtggtcgtc gcccgctgga
     6001 tctattgccg cgtcgacggc cggcgcaagc tgccgccggc cgcctggccc gcattcgtca
     6061 ccaacggcgt gctgcccctc ctgggtcaga tcgaggaggg gcttctgcgc ccgcaaaaat
     6121 ccaatgcccg gtgattgatt cctatgatga actccgcctc cgattcgatt ccggcctcgg
     6181 cgcttgttct cggggcctcc ggcatgatcg gcctgccgct ggcgctggcc ctggccgaac
     6241 gcggcgcacg cgtctacggc gccgcgcggt tctccgatgc cgcgaagcgg cggatgctcg
     6301 aagaacgggg cgttgcaatc gttccattcg acgtcctgcg cgacgacccc gccgcgctgc
     6361 ccgatgtcgc ggcgctgttc ctcgagatct gggatcccgc caaccccaag ctgacgtttg
     6421 agctgaacgt ccacgccgtc ggacgcgtcg tggagcggta tgccggcgcg gccacgatcg
     6481 tcaacggcgg cacgatcaat gtgtacggcg ccggcgacta tctgccgcgc gaagacagcc
     6541 cctgccagcc cgattcgccc tacggcctga gccgcttcgt tcaggagcag atgatcgagt
     6601 ttttctgccg ccaacgcggc ggaaaggcga tccacgtgcg ctacgcgcat accaattccg
     6661 cctcccgggg gatgatccac tggatggcga gtcgcattct tggcggggag tcgctcggtc
     6721 cccaccccga ccgccggatc caggtgatcg cgctggagga tgccgtccgc gtcacggcgg
     6781 cggcctgttc gcgcgccgaa cacccgaccg cggccgtcaa ctgctgtcat ccgcgcgaat
     6841 ggggatggcg cgagctggcc gaggaaatcc accgccggct cggacgcggt tcggtcgtct
     6901 tcgatgcccc cgccggcggg gtggcgacga ccgtctgcgc cgacgccgga cggatggtcg
     6961 aatggttcgg cccgccgcag gtcacgctgg aggcgatgat cgagcgggtg gtccgggagc
     7021 tgagctgacc gcgcgtggat ttcggggaat tgaatgatcg aaccgataaa tcggttcggg
     7081 gttgttgggc gtgccgaccg tgagacgcat ggcgccgagc ggccatgcaa cccacggctt
     7141 ggataccgaa cggctgacgc ggttcccaac agaaatacgg attcgaactg cggcaaaccc
     7201 ccacgggcca cggtagttga tgcaagtcac cggggggtgg cttgggtgag ctggcgcggg
     7261 ggttgcggtt ggccggtcag gccgtgacaa tcggtgcagt tgttgcccag ccagatgtcg
     7321 ccgaggccgc gagggtggtt gaactcgccg ccgccggtgt agtcggcggg ggcgaaggcg
     7381 gcggcgccct cggcctggtc gagaatttcg tgacacagtc ggcagtcgtt ggagatgaca
     7441 tggccggcgc gggttgccgg cgccgcctct ccggccgccg cccgcgccgt tccgttcggc
     7501 tcggcttccc gcccggtccc ctgcgcggcc cgcgcgacgg cctcgcgcac ggcggcgggg
     7561 gcgcggtcgg agacatggcg gtcgtcgtgg cagcgatagc agccggagaa ttcgaaatgg
     7621 ccgagatggt tggggtaggc gcgccagtcg acgccttgct cggggaagaa attgcgctga
     7681 tagagccggg cgagccagtc gatgttggtt tcgaggacgc gggcggcttc gggtccctgg
     7741 acgcggtcgg cgtagcgttc gcgcaggccg tcgatcgccg ccagcgcgga aggggtgtcg
     7801 ggccagggct gttcgaagat ttccgttgcg tagcgtttca ggtaagggag cgagtcgtcg
     7861 agcacgccgg tggccatgta gaaatcgatg agctgcttgg gggagcggta gatgtgcgag
     7921 ggccggttgt ggcaatcgat gcagtccatg aggcggagtt cggcgtcggg ggggaggggg
     7981 tcggggcagt cgggcgagcg gaaggattcg gtggagccgt cctcgcggcg gacctcgacg
     8041 taggggatgt caaggcgctg gcgatcgcgg gcccagtagc gaacctcgat gctggggttg
     8101 atgtgccagt ggatgccgcg gcccaggccg gcgtcgggca tgccgccgcc gatcttgagc
     8161 agcaggttgt agcgcgccgg ggtgttggcg cggtcggggg agaagtgcca gatgattttt
     8221 tcgacggagt cggagaactg ggcgggccag tgacattgct cgcaggtgtg gcgcgcgggg
     8281 cgcagattgt ggaccggcgt ttcgatgggc agttcgtagg atttggtcag gacggcgaag
     8341 acctggcgca ggccggacat cttggagcgg acataccagt cggcgccggg accgatgtgg
     8401 cactgggtgc aggcgacccg cgcatggggc gagtgctgat aggcggtgta ttcgggctcc
     8461 atgacgtgat ggcagatctg gccgcagaag acaggcgatt cggtgaaatg ataggcctgg
     8521 taggtgccga ccgaggagac ggtgaacagg agcgaggcga tgccgacgcc ggccaggatg
     8581 cggcggcgct gccaggggag gttcaggtcg atgagcggga ggcgggggta ggtggagggg
     8641 gccttgcggt ggcgacggcg ccactccagc gcgatgccgc cgaggatcag ggcgatgccc
     8701 gcgccgagga cgccgggcag gacgaggtag gtgacgaggc tgttataggg attttcctgg
     8761 tgctggagga tatccatcag gatcaggacg acgagggcgg cgccggtgat ggcgacgacg
     8821 atccagccca ggtaactgac ggggttgcgc gagagcgggg gcggcggcgc ggtgggatcg
     8881 ggatcggggg gcagatcgtt cggcatggaa agttcctggt tttcggattc ggactggacg
     8941 gtctcttgct aacagggcgg aggcggaccg cgaagggccg agagagcaag aatggttcca
     9001 ttgaagatac ggagatattt ttatcttgta gttagagggg gcgggtttgc cgccatggcg
     9061 ttgttcgccg tctggggcct ggcggcgggg gaagcggcgg ggtcggatat gccgcatgcc
     9121 ggccaactgg acagccattt tgaccagaac tgctccaccg ccgattgtca cccgcacgcg
     9181 ccggccgccg acgccgtgcc gcacccgccg tatctggaga acaactgcct ggcctgccac
     9241 gaggaccatg cgagcagcgc gacggccctg ttgaagcccg atgtggaaca ggtgtgtctc
     9301 gcgtgtcaca cgcaggtgca tcccgaggcg cggccggcgg cggatcaccc gccgggcagc
     9361 agcgactgcc tgcgctgcca ccagccgcac cagagcccca cgcgcggtct gctgcgcgac
     9421 gccgaggtgc tcgggatctg cgccgactgc caccggccgt tcctggagga ggcggcgacg
     9481 ctgccgtatc ggcaccgcca tttcgacctg cagggccagt gcggcaactg ccactacgcg
     9541 caccgggagg cggaggcgag ctacctgcgg gccgaggtga ccgagacgtg cctgacctgc
     9601 cacgacctgc cgatccagag cggcggccgg acgctggaga acgtggcgcg ggagctgcgc
     9661 gagcagccgg tggttcacgc agcgatcgat gaggggtgcc ccacgtgcca cacgccgcac
     9721 gggtcggtgc agcccgcgct gctgcacgag gggtatccgg cggggaatta cggcgcctat
     9781 gaagaggaga actaccggct gtgctggcgg tgccacgacg cggggctggt ggagcaggcg
     9841 gatgggcggg gggcgacgga gttccgcgat ggggcgaaga acctgcaccg ggtgcatgtg
     9901 gcgaatctga agcggggccg gacgtgccat ctgtgccacg aggcgcacgc ggcggaccga
     9961 ccgcacctgc tgcggcggac gatgaaattt ggcgcgtggg aagggccgct ggagtggcgc
    10021 gagacggagg ggggcgggag ctgccagacg ccctgccacc gggaggcggg gtatgggcgg
    10081 caatagacga cccgtttatc agaaatagag cttgacgacc gggccataac aagatatata
    10141 gatctgctaa tcgaatgaag tccgcatccc gatggaaatg aaagaacccg acgcgatggc
    10201 cgccaaagtc caacaccacc gcccgcccga gcccggactg ctcgactggc tcagctcgct
    10261 caaactgggg ctggtgttgc tgacgctggt cgggatcgcc tcgatcgcgg cgacgctgat
    10321 cgatccgctc cccaaggcgc agcaactggt gtatttcacc tggtggtaca aggtgttgct
    10381 gctgctgctg gcggtgaaca tcgcgtgcgc gacgtggcgc acggtgacgc ggcgcgtgct
    10441 gccgctgcgc cggctgcggc cgatgcggca gaaggaatac taccggacgg cgccgctccg
    10501 aaccgacatc gcgtttcatg gcacggccga ggcggtggcg caggccttcg gccggcggcg
    10561 gttccgcgtg gcgcgagagg gggaattcgg gcacgcgcgg cgcggctatc tgagccgatg
    10621 gggcgccccc gtttcgcatg tcgggttcgt gatggtgctg gcgggcgggt ttttgtcgcc
    10681 gttcttcatg atcgacggct acgtgcggct ggccgagggt caggcgaccg acaccgtcca
    10741 tctgcgcggg gagaaggggg ggacgctggc gctggactat caggtgcggg tcgaggattt
    10801 cgatttgacg tacttcccac aaaccgagat gccggccagt ttcgtttcga cggtgagcat
    10861 cctggagggg ggccggacgg tgaagacggg gccggtcgag gtgaaccggt cgctgaagca
    10921 cggcggatta aaattccacc agaccgggta tggcgagcag ccgtcgctgg cgcgtcacgc
    10981 ggtggaattg acgcacccgc ggcaggcgcc ggcggggatt cagtttgagc tctcgccggg
    11041 tcaggtgcgg gcggtgccgg gtctgccggg gtatgagatc gcgttgcacg cgcacgcgcc
    11101 gccccactgg gtgttgagcg acgcgacggg gccggtggcc gagggggcga tcaggggggt
    11161 gagtggaccg gtcacgatcc gggccgagcg gtttgagccg gacttcgtga tcgggccgaa
    11221 ccgccaggtc ggcagccggt cggcggagct gaggaacccg gcgctggaag tgagcctgat
    11281 cgagggggag acggccgcca tccgccgttg gctgttcggc aagccggaaa tgcgggccat
    11341 gagccacggc ggcggcgggc cgttcgacct ggaactggcc gaggtgcggg gcgaggggga
    11401 gagccggcgg ttcgtgctgc gggcgctggc gcccgacacg ggggtggagg tggaccgggc
    11461 ggagttggcg ctgggcgagt cggcgacgct gggcgagcaa gcggcggggg aaatacccgt
    11521 ggaggacgcg acggactggc ggctggagat tgtcgggcgg gcgccggcct acacgaccga
    11581 cttgagcgtg acgcgcaatc cgctgatccc gttcatctac ctcggctgcg ggctgatgct
    11641 gctggggctg gtgctggcgt ttttcgtcaa gcagcgcgac gtgtggttcc acgtcgacgc
    11701 ggcgcggggg cggctgtggg tggtcgcggc gtatcggcac ccgcacggcg agctggaccg
    11761 tgtggtgcgg tcggtggtgg aaggtttgcg cgaaccggag gcgatcccgg ggggtgcgcc
    11821 ggcaagtgag cccgaagtgg cgcgggcggc gacggcgtag gcgaggcgga gggttgaccc
    11881 catgatggaa cgtgaaagga acagacgatg agcgaagcat gccggcggtg gcgggacatc
    11941 gacacgagcg ccgggtcggg gctccggccc agttaccggc gggtgatctg gattgcggcg
    12001 tggctggcgt tcgtggcggc gatgtggacc gtgccggcgc ggggggcgga gagcgcggcg
    12061 cccgcgatga gtccggcgtg ggaggcggcg ctgcggccgc tggagcggct gccggtccag
    12121 gataccggtt atgttcgcag cgcgcacacg tatgcgcgga cgcggctgcg ggggatcacg
    12181 gggcggaacg gtctggggga gctgaatgcg gtgcagagcc tgatctggct ggtgtcgcat
    12241 ccggcggcgg cggcgcggga accgctgatc cgggtgacgc atccggagct ggggtcgctg
    12301 ctcggggggc ggcggacgag tctggacacc taccgtaacg gccgggtgcg ggagcggatc
    12361 gtggcggcgc tgcgcgccga cgagaacctg ctcaagccga tcaatgagct cgagacgcgg
    12421 gcggcaatgt tcgagcatct ggaagcggag atggcgttcg tgccgcgcgc gggggaatgg
    12481 ctgtcgccgg tggacgccgc cgggcgggac gacctgagca gcgaggagcg cgaactcgtt
    12541 gaactgtggg cggctctgaa gcgggccgtt gccgaggaca acccgacggc cgggggggaa
    12601 ctggcgcgcg agctcgcggc gcgggcgcca aggctggcgg aggcgcgggg cctggcgctg
    12661 ccgcggctgg aggcggactg gttctatcac acgcacgagc cgttccggaa atcgtcgttg
    12721 ttctatctgg tgggcgcgct cgggttcggc gcggcgtttc tgttgggacg ccgctgggtc
    12781 tactggtcga gcctggcgct gatgggaggc gggctggccg agcaggtgct gggggtgggg
    12841 ctgcgctgga tcgtcgcggg gcgggcgccg ctgtcgaaca tgtatgagtc gttcacgttc
    12901 gccacggcgg ggatgatcct gctggcgctc gtcttcgaat ggatttaccg gacgcggctg
    12961 gcggggctgg gggggacgat tctggggttt gcgttcatgg tgatcgcaca caatgttgcg
    13021 atcttcgaca gccagatccg gccgctgatg ccggcgctgc aatcgagctg gctgacgtac
    13081 cacgtcgtca cgatcatgct gagctattcg gcgttcgcgc tgtcgttctt tgtcgcgctg
    13141 gtctatctgg cgaaggaggc gctggggggc gacggctcgg tccgcctggc gctgcgcaag
    13201 ctgccgagcc tgcaggcgct cgatctgttc aactaccgga tcattgccgt gggattcccg
    13261 ctgctgacgc tggggatcgt gacgggggcg atctgggcgg cgacggcgtg ggggcggccg
    13321 tgggcgttcg atccgaagga aacgtggtcg gcgatcacct ggctgatcta cgcgatctac
    13381 ctgcacgtac gctacatggc gggctggcgc gggcggcggg cggcggtgct ggcgctggtg
    13441 gggttcgcgg ccgtgctgtt cacgtacctg ggggtgaatt acctgctgcc gggattgcac
    13501 agttacgcgg cgtgagggga gaggaagggg aagagattct tgtgaatcgt tgggggcgat
    13561 cacgaggacg atgacgagag acgagggacg aaaatggggg agagagggga cgcgatgagc
    13621 gaatggatca ggttcagtac ggaagtcgtt cggccgtcgc ggcgcgatgt tggaggcgct
    13681 gtgacagccg cgccacagga ttcccagccg acgctccacc tgtgcggaat ccaccattcc
    13741 acgtcgccgg tagcgatccg cgaacgctat gcgctggcgc ccgatcaggt cggcgcatgg
    13801 atgcggcgcc tgcgggaaga gacgggggcg caggtgctga tcgtgtcgac ctgcaaccgg
    13861 atggagatct atgcgtgggg aagcggggcg ctggggaacg agctgcgcgc ggcgatgcgg
    13921 cgcatcggtc tggccgcggg ctgcgcggag gcgccggagc tgatcgagcg gtcgggcgag
    13981 gaggcgctgc gccacctgtt ccgcgtggtg gcgggcctcg attcgatgat cctgggcgaa
    14041 aatcagatca agcagcaggt gcgcgaggcg accgaaatga gtcgcgcggc gggttgcctg
    14101 ggcagcgagc tgaatcaggc ggtggaggcg gcattccgtt gcggcaaacg gatccgcacc
    14161 gagaccgagt tgaatgtcgg gacgctggac gtcggcaagg cggcaatcct gaaagccgag
    14221 gaacgcctgg gcgggttgac gggccgggcg tgcatggtca tcggagcggg caagatcgga
    14281 aggacggcgg cgcgggcgat cgcggaacgc cggcccgacc ggctgtggat cgtcaaccgc
    14341 tcgctggagc acgcgcgcgc gatcgcggag gagttgggcg gcgaggccta cggcctggag
    14401 gcgctgggct gcctgctgcc cgaggcggag ttcatcctgg gcgcggcgca tacgcccgag
    14461 ctgctgttcg atgccgagga ttatgaagtg gcggcgccga cgggcagccg ccccgagcgc
    14521 gtgtgcctgg tggatgtggc ggtgccgcga atgctcgatc cgaggctggg cgaattggcc
    14581 ggcgtggagc tctacggtct ggaggacatg gaggagatca ccgcggtcaa ccggcggcag
    14641 cgcgaagcgg cggcggaagc ggccggggtg atcgtggagg aggaactgcg gcagtggcgg
    14701 cgggagcggg ccggaggggc gctgggaccc gcgattgagg agttgaaggc gcggctggag
    14761 acgatcttcg cggaggagga agccgcgctg gccgaagcgt tgggcgaggc gccggcggac
    14821 cgattaacgc ccgcgctgcg gaagcttcgg gggagaacgt tacacgccgc catcgagtcg
    14881 ctcaagcggg cggcgggggg cgattgctga gtgagagggg ggcgtaagcg gcgcaatcgg
    14941 atctccgatg tcaggtagtt catggcgtgg ggcgattcgg ggcgattagc aggttgctca
    15001 ctaacctgcg gttcagaatg aggtgacaaa acagccggtt ttcgagagtc gaggacgagg
    15061 gacggatttt tgagtgtggg gcgagagtcg tagacatggg gtcggacccg ccccctggct
    15121 cattcgaccc gggcgcggaa gggggaggca gggaggccgt tctgattcat cagattgggg
    15181 acggcggtgc gggaccaggc gaaacgaacg gcggtcgggg tggtgatgtc gtcgctccag
    15241 acgacgacgg cgtcgccgac gatgtccgcg cgcgctggca cgaattgccc atccgcgccc
    15301 gcgacctcaa acagatcggg atccttcccg tcgcgcgtgg agaggccctg gccgacatag
    15361 tcaaactcga tccgcaggcg gttggcctcg acggcgagcg agcggtagat cggtcccgtg
    15421 ggaacgatgt cgtcgcggcc ataggtctcg gccagggccc agagcgccag ccggcggccg
    15481 acctcctgct tgttgcgcgg gtggatgtcg tgcaggtcgc cgacgtcgga gatgacggcc
    15541 atgccggtat ggggaaccgc gcgcatcacg gcggtctggg cttcccacag ttcgggcagg
    15601 gcatcgccgg cgtacaaggg gtccaggtcg catggggcga tctgaacgta atagaacggc
    15661 agttcgggcc ggccccagag ctggcgccag ccggcgatga gggctttcat tttctcgaaa
    15721 tacatcatgc cgtcaccggc gttggactcg ccctgatacc agatcgcgcc gcggagggcg
    15781 aaggggacga gggggtggat catcccattg tagatgccgg tggggcgttc ggcggaatcg
    15841 agcgggtggc ggggccagag cggcggatcg ggcacgcgtt caccggcatc gagcgcccgg
    15901 cgggcctcgg gcagccagct ctcatattgc gggacggcag tggacaggtc gtcgcggaat
    15961 ttggggccgg cctgctcgat ctgcagcaga tagtcggcca gggcgggatt cgaggcatag
    16021 ccttcgggcg ggatccagcc ctcgatgcgc gtgccgcccc aggcggcggc gatcacggcc
    16081 acggggacgt cgaggtcctg gtgcagcttg cggccgaaga agtaggggac ggcgggaaag
    16141 ccgccatcct gggcgatggt cgccgggctg caaacccgcc aagtagccgc gacgtcgtcg
    16201 gccggaacgc cggaggagcg caccgggacg ttgaacagcc ggaggcgggg ataattggcg
    16261 gcctgaattt cctgctccgc gtcgcgcacg acgaggatgc tccactgcat gtttgactga
    16321 ccggcgcaga gccagacttc accaatcagg acattttcca gcgcgcgcga ctgttcgccg
    16381 gccgtgatcg tcacctggtg cggcccgccg gcttccatcg gggggagctc gacgcgccag
    16441 cgcccgtccg cgccggccac ggcagcggcc gaattgtcgc cgagggtcac ttcgacttcc
    16501 tggccgggac tggcccagcc ccagacgggg atgggcgcct cgcgctggag gaccatgtta
    16561 tcgccgaaga gctgggggag ggttaattcc gcctgggccg tggcaacgag cgtgagcagc
    16621 aacacgacca gcatggggat ggagatcggc gagtcaaggc cagccgtacg tcgcatgttc
    16681 aacgctccct tcggtggtgg tggttcgtcc ggccgggacg ggcaatcggg tgccatgctg
    16741 aaccttgcgc ggcctgaagt caatgatcac ggggaaagca tccttcgcat ccctattgtt
    16801 cggttcccgt agtacatccc ccggtttggg ggcgcgagcc gggtggcgcg ggagcatgcc
    16861 ggtgctgcga agtgcagcgt atgcatttgg ttgcagcggg ccttgcagtt gacccgccaa
    16921 ttgacgtttc cgcgtcgcgt cgccgccgcc gctgatcaat cttatccgtt gattttacga
    16981 ggtttacgtc gcgctcgcgg accgatccgc gtcgggcccg caatttgcat tgggggctct
    17041 gcgtgttttg agtttcgacg ggaggagcac gaggaaacat gcgacgcttg caccgatatg
    17101 cggccatcgc cgcgtccctg ttgctgaccg cgatttggat cgcgccggcc caagccagtc
    17161 tgatcggaga tcggatcgat tacagttttc aggatgataa cggtttttct cagtcgggtg
    17221 aggatctact ggttcggtcg gggagcgaga tatcgctgac cgcgccgctc tatcggctcg
    17281 acgtcaatct cggcagcgac tgggttcaga tcaatcagaa ctcgcgcagc gcggccgacg
    17341 ataagtatcg ccgcacgggc aagtgcacgt tcccgggatg ggaactgctg ctggcggatc
    17401 tggatctggg cgaggaccgg gagatcaccg gataccgact gacggcctcg gggatcgacc
    17461 ggaacctgat cggcgtcaac ttcacggccg attcgctgac cgtcgagttc gcggggctga
    17521 cctatggctc gggcaagaat tccaagagcc tgtggaacga tctgctgaag tgctatgacc
    17581 cgtgcaagct cgatttctgc ctgaagatcg atctgatcac gtcgcccaag ccgccggtca
    17641 ttccggagcc ggcgacgctc ggtctggtga cgctgggggt cggcggcgtg gggctgctgc
    17701 gccggcgcgt gaaaaagctg aagtaggacc cgggcgcgcg acgttcgcgc gcggcaacgg
    17761 gaagaccgga ggggcccagc gcggctcttc cggtcttttt tttgcggacc aaacggcaaa
    17821 aaggagaatg ggactttaga aaatagaaag ttgttataga tgagattcga ttatctgacg
    17881 gcctggccgg cgggggaagg ggcgggccgg tgactggccg ggcgcggggg gcggggacga
    17941 catttttcag ccagcccccc ggattccgtt ttgtcccttg gactatttgt gttaaagcta
    18001 agtctcacga cgtgccgaac gggcggtggc gcaacgccga aacgcggcag ggaaccatgc
    18061 aggacggccc gcgaaccagc cttcaagccc gaatccaggg acgggtgcag ggggtgggtt
    18121 tccgcttttt cgtgctgcgc gaggcgcgcg gtctggggct gacggggacg gtggccaacc
    18181 gtcccgacgg atcggtggag gtcaaggccg ccggcgggcg cgaggccctg gaggagttgc
    18241 tcggccggtt gcggcggggg ccgaccctca gccgcgtcga tcacgtcgag gtccaatggg
    18301 atatcccgct cccggacatg tccgaattca ccatcgagtt ctgagccgat gctgctttat
    18361 accgatctgg tccgtgatat cgcccgcgat gtggcacgcc gcgtccccga cctggcgcat
    18421 atcgatgccg accggatcgc gttcctggcg gcggcgcgct gcacgagtca ccgcacgggg
    18481 aatctggcga tctgttacgg gctgaatcgc caggccgagc cgacgttttc ggtgtgggtc
    18541 cggcgcggga cgcgcaaggt gatcgccgtc agcggctggg ttcagatgcg cgtgccgcgc
    18601 gtgtacatca acgggcgcga gatgagctac ctgatcctgc tgcggctgcc gcggctgctg
    18661 cggcagaacc cgctggagac gatcatccac gagctgtatc acatcagcga ggggtttgac
    18721 cgccggatgc ggccggcgcg tcacggggcg ctgttcgacc gcgaggtccg ccggctgatg
    18781 aacgtctggc tggccaatgc gcgcggcgat ctgccgcggc tggcgcagat gacgctgcgt
    18841 caactggagg gggagttcgg ggcggtgctg gcccaggggg tgccgtccaa cttccggctg
    18901 ccgatcgtcg agccggtgcg cgggccggaa tcgtatcagc gcgcggtgcc gcggctgtat
    18961 ccgggccaca gcctggcggc gctgtatcag gtgcggccgg cgcagcagct ctcgccggag
    19021 gagacgccgc gcgtgctgac cgagcgcgac ctggtgctgc gccactacca cacgcgcggg
    19081 gtggagcgcg tgccggccga attcacccgc tactcgcgca acggacggcg cagcctcgcc
    19141 ctgagcgcct gaccggcgtc ccgcatgcga tccgcctctc cccgaccgcc cgggttcgtc
    19201 cacgtcgatc tggacgggct gtggacgctg gccggcgtct atggctttcc cgagcaagat
    19261 ctgttcgaac tcgacccggt gttcaatctt gccgtcgacc ggctcctgga gctgctcggt
    19321 tcgcggggca tccgggccac gtttttcatc gtcgggcgcg atctggagct gccggagaag
    19381 gtcgatgcgg tcgcgcggat cgccgcggcg gggcatcgcc tggcgaacca cagcctgcgc
    19441 catgacctcc ggctggggga agccgcgccc ggaagcattg aagaagagct gcggaccgtc
    19501 aatgagcggc tgagcgccgt ggcgggggag gcgccgctgg ggttccgcgc gccgggctac
    19561 gccgtcaacg agcgggtgct gacggcgtgc gtgcgggcgg ggctgcgcta cgacggctcg
    19621 gtgtttccct gctggtgggc gccgctgctg cggcggctgg cgggttcact gcgccagtcg
    19681 cccgggccgc cggcgcgggg gcaatatggg ggggccgggt cgctgcggcc gcgctgggag
    19741 aggcccgtgg cgggggcgcc gccgatcctg cggctgccgg tcgcggtctc gccgcgactg
    19801 cgtctgccgc tgcacgccag cctgggtctg ctgctgggcg ccggcacggt gaggcggggg
    19861 ctgcgcaacc tggccgcgcg cgggctgccg gcggtctacc tgctgcacgg gctggacctg
    19921 ctcggggccg aggagttcgc cgggcgactg ccgggcccgc tggccggcca ccgggcgttc
    19981 cgcccgtcgc tcgccagtaa gcgcgagttc ctggataccg tgcttcgcgc gttcggcgag
    20041 ctgttcgcga ttgagacgac ggaggactgg atgcggaggc tggaggttgg gaggagcggg
    20101 gggatatcag cgtgagactg gcattgaaat gggtgggttg cgggggaaac cgttgaaaca
    20161 attaggaata atatgtcgcg tctagtaacc ggaccgaggg gtgccaacgt cagggccccc
    20221 gaaggggcca gaaaaaaacc cactcgcggg ctcacaagcc ggccgctcac cctcaagcag
    20281 aaacacgacg ggggcagggc gaacggcagt cgagcgacga ggacgagaga cgagggatgg
    20341 atttggtgga ggggcgtgac tttggcgggg cgggggcata gaatgatcgc tcgagcatga
    20401 gggcgaggca gaacagtggc gggcaagaga tcagcaacgg gacgggcggg acgcagcgcg
    20461 ccggccggcg gcggcacgct tcaggaattt cgcggcctgg tcgaggagta ttacgagcaa
    20521 acattcgagc ggtttccgat cgaaggctcg cgggcgggcc ggcgggagtg tgatggcgag
    20581 ctgggccggg cgcggccggg cgatttcgaa cgccaggggc ggctggtcga gcggatcctc
    20641 gccgccgtgg aggacctgcc gccgcaggat ttcgacgcgt cggcgatgct cgaccggcgc
    20701 acgttcgtcg ccgcactgcg gctggaacac ctcaacctgg ttgaactgga gcgctggcgc
    20761 aatgatccgc aacgccacct gcacgccgtg gccgagtcgc tgctggagct ggtcgtgcgc
    20821 aacgccgatc ggctgggggc ggtcgccccg gggatcctgt cgagcctgcg ccaggcgccg
    20881 cgctattttg gcgacgccgc cgaatgcctc gaccgtccgg acccgctgtg gcgcgagctg
    20941 acgctcgagg caatccccgg cgtgatctcg tttttcaatt cgctggcgga gccgttgatc
    21001 cagacgcaag ccgccccggc gggagtgatc cgccggacgg tggcgggggc atgcgccgcg
    21061 gtggaagcct acggcgacgc cgtgcgatcg acccgcccgg gcaaggcggg gtcgtttgcg
    21121 gtcggcgagc ggcggctgcg gctgctgatg cgcgaccggc tggggctgga ctggtcgccg
    21181 cgcgaggcga tggcgttcgc gcggcgggaa gcgacgcgga tcgcggccga gctgcgccgc
    21241 gaggcagcgc atttccatcc gcgcaagagc ccggtcgaga tcctggccga ggccgcggcg
    21301 gcgtggcggc ccgacagcgg cttgctggag gcctacacac gggtgacgcg ggccgtgcgc
    21361 gagcggtttg aggaggccga gctggtctcg ttcccgaagg ccgaacggct gctgatcaag
    21421 ccggtgccgg acttcctgcg cgagctgttt ccgacggccg cctacacgcc gccgggagcg
    21481 gtcgatccgg atcagaccgg cattttctgg gtgaacgacc cgggggacgt ggacctgccg
    21541 ccggcgcggc gcgcggcgga aatcgcccag catttcgggc tggagatgac ctgcgcgcac
    21601 gaggcctacc ccggccatca tctgcagttc atggtgcagc accgccggcc gtcgttcgtg
    21661 cgcaaactgg ccaatcacgc cgtttactat gaaggctgga cgctgtggtg cgagcagatg
    21721 acgtgcgatc tgctgagcca gggggtcggg cgcgggcggc gcgcgcgctt gcgcgtgccg
    21781 ggggcggggc tgccggagaa tccgcacatc cggctcaacc agctcaacga tgaactgtgg
    21841 cgcgcgtggc ggatcgtgat cgacgttggg ctgcagacgg gcgagctgac gtgcgaaaat
    21901 gcgcgccgga tcctgcagcg ggaagttggc ttcacggccg cgcgcgcgcg cgccgacatc
    21961 aactggtata cgtcggcgcc gacgatcccg atgagttacc tgatcggcaa ggccgagctg
    22021 ctgcggctga agcggcgcta tgtcgacagc ggcgggctga cgctgcggga gttcaacgac
    22081 tgggtgctga gcttcggggc gatcccctgg cgctggatcg aggagagcgg gttgtagccg
    22141 gggatgattt gagatggcgg gttgcgagac gacgatcgaa ctcgccgcgc ggcaagatct
    22201 ctcaggcagc ggacatcacc tgacaattag agttggatga cggagaggtc gatttcgcgc
    22261 tcgcgccagc cgccgacggg gatctcgcgc gcgcnnnnnn nnnnnnnnnn nnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnacggag aggtcgattt cgcgctcgcg ccagccgccg acggggatct
    22441 cgcgcgcgcg gagcgcggcg agccatgggg ccggatcctc gagaggctcg cgcgtgctga
    22501 agacgacgtg gtcggcaagc gattgcgcct gatcgacgcc cggcgcggcg cggagcgtct
    22561 cgcgcgcctg ttcggccagc cgggcgcgcc gttcgggcga atcggtctcg gcgccgatgc
    22621 gcacctcgaa ggcgcgggcg ggcgccagcc ggcgcaggac gtcggccatc gggccctcga
    22681 ggacgatgcg tccgccggcg atcacatagg cccggctgca cagtccgacc aggtcggaga
    22741 ggatgttggt tccaagcagg agcgtgcgat cgggggcgag cagccggtcg atcagctcgc
    22801 gcaggcggcg gcgggcggcg agcggcagac cggcggcggg cgagtcgagg atcagcagcc
    22861 gcggctcgtg cagcagcgtt ttgatcaggc ccagccgccg tttctcctcg cggccgagcg
    22921 tttcgacaaa ggcctcgcgc tggtcggtca gttcggcttc ggccagcaga cggtcgatgc
    22981 gcgacctgat gtggcgcggt tcgacgcggc cggcgcgcgc gaagaagtcc agatattccc
    23041 agacgcgcag gtcgtcgtag gcgcccagaa cgtcgggcat gtagccgacg cgggggcgga
    23101 tcgcgtcggc ctggctgagc aggtcctggc cggcgacggc gccgccgccg cgggtgggac
    23161 gcgtcagggt cgccagcgtc ttgagcaggg cggacttgcc cgagccgatg ggtccgatca
    23221 ggccgacgcg ctcgcccgaa ctgacctgga tggaaacgtc gcggaagatt tcgtagcggt
    23281 cgacctgaag ggtcaggttg gaagtgacga tggccgggcc gtccgggaag gcgcccgtgc
    23341 ctggggccgg ggcggcggcc gcggccgcgc catcgcggct gttgttgtcg gtgtcgctga
    23401 cgctggtcga acgggtcatc gccgcgcctc accggctgca tgaagatctc gggcgccgaa
    23461 ctcccggtcg agcggtgcgc ggagcggaag acgctcccgc gcggtcgatt gccgcggggt
    23521 gggatgagcc ccatggagcc ggcggtgacg gtctgcatcg tagccgccgg ggggcgtgag
    23581 aatccagatc aaagagggcg ggttcaacga aggcaggctg gaatgaggcg tgataccggc
    23641 ggaggcattc acacaggaaa tgagtctcac gccgagacgc gaaaagtcca atatcataat
    23701 gggttcatcc acagcgggtg acaacgaatg gatacgatgg cgggttttcg agagccgcgg
    23761 acgagggacg agagccgagg gccgaatttt ggggcgggcc actgggggcg attacgagtt
    23821 gctcatgaac cgggagttca cgataagggg acagaattgg gtagggcttc gggagcgatc
    23881 acgaggacga gggaggagag acgtccgggc taggggcggg ggaggcggtc gatggcggtg
    23941 aacgggcggc gggtggtctg cttcttctga atcgcttcca tgatgcggcg gtcgaattcg
    24001 cgggcgacgt tgatcccctg atcctccaga cgctgggtga gcgccgccac ttcgatcagc
    24061 ttggcgatgt tgcggcccgg ctccaccggc agcacgtatt ccgccacgct gcactcgaac
    24121 aggatcgact cgcggtcgtt gaggccgagt cgctcgtatt ccttcatcgg atcccacggt
    24181 tccatcatga cgcgcaggtt gatgggggat tcctcgcgga cggcgcggac gccgtagagc
    24241 agctcgacgt cgatgatgcc gatgccgcga atctccatat ggtagccgat gttggggggg
    24301 gtctcggcga tcaactggtt cttgctgatg cggcgaatga cgacgacgtc gtcggcgatc
    24361 aggcggtggc cgcgctcgat cagttccagg ccgcattcgc tcttaccgac gccgctgcgc
    24421 ccctggatca gcacgcccat gccgaagaca tccatcatca cgccgtggac ggtccacgcg
    24481 ggggcgagcc gccgttcgag gaaatggctc aggtcggcct ggaacaggct cgtggtcagg
    24541 ctggtgcgca gcagggggat ctgcagcgcg tcgcaggtgg tgatgagctc ctcggcgatc
    24601 tccagatcgg tcgtgacgat cacgcagggg atgtgaaagg cgaaggtctg gcggatgacc
    24661 tgctggcggg tctcgggggt caggctggcc aggtagcgcg tttcggtgag gccgatcagt
    24721 tggatccgtt cggcggaaaa gacttcgtag tagccggcca gggcgaggcc gggccgattg
    24781 atttcggggg tgtagatgac gttgctcagc ccgtcgcggc cggcgacctg gaccaggtcc
    24841 caatcgcgcg ccttgcgcga gagcaactcc tcgactttga ggggttcatt gagttcggag
    24901 atgggcgaaa ccattcgcgg cagaggactt tcgtgaaatc cgcggcgggg ccggcccccc
    24961 tcggctggat catcatagtc aggcggcgcc gacggggcaa gcccaccttg gccagtctga
    25021 ggagttcggg ctctgaatgg atggtgttgc ggggtgggtc ggcgggctgg aatgagaaaa
    25081 aatcaggttg gtgaatcgcg attttggtgc taagtttata agcatacgga catcgcactg
    25141 tgaggccggg ccgaacgcgg agcaacccca acccaaccgc agacgtcgtc gccccccttc
    25201 ggcactgatg tcggatccag ggatgctggc atagaggcca gaaacgtcct tttgaacttt
    25261 cgaaaccccg aagaggtgat ccatccatgc aactggcacg cttcaacatc ggcgactcgg
    25321 ttcgcctgcg cgagcgcatc catcccggtc acccctatgc gcggcttgac cagcgctcgc
    25381 cgaatcgcag cgacattccg gcggggacga gaggcattgt gcgccgctcc cacggcacca
    25441 ccgtgatggt ggagctgttt cccaatccgg acatcatgac cggccgcggc atcgatgtgt
    25501 gggtcagcta ccacatgctg gtcgtcctgt tcgcccccga tgggaccgga gcggaaggga
    25561 aggacgcgtc gatggccagg aagaagagca cgggcaggct gatccacgtc taaggcgcca
    25621 gcgccctcgc acccgcgacg aggcaaccca tttccggcgg gcggcttgac ccggtagtca
    25681 ggccgtccgc ttttgcattg ccggcccctg ggagtcacca ggggttgccg gattcccctt
    25741 cctccagatc gtccccgtcg tcgggcagct catcctgatc gcgcaggcgc tggtgatagc
    25801 gggccgtggg cgggacgatc ccgttgagga aatcggttcc gtcctggccg agcacgccct
    25861 ggaccgcttc atcgaagaac tggcgcgggt cctggccctt caattggccc atcttgatgc
    25921 agtagcgcag gatattgacg ccgtcgcgcg aggtgaagcg caggttgagc cggtgcgcct
    25981 tctgcaggaa cgagacggcc atttcgagca gctcgtcgcc gctgaacggc aggttgtaac
    26041 ggaggatctg gagttcctcc tcgcggttgg ggaaggtcac ttcgatctgc ggctggaggc
    26101 gcgactggat gtattcgggc acctcgtagg tcgaggcgtc atcgttcatc gtgcagcaga
    26161 tgcggaactc ggggctggcc tggatcttga ggccggtgac gatcgattcg acgtagcggc
    26221 gctggtcgag cagcggggcg agcgaggccc acgatttctc gctcatgcgg ttggcttcgt
    26281 ccaggatgca gacgccgccg cgaatcatcg ccgtcaccag cgcgctggca tggtaggcga
    26341 tccgcccgtc gctggcgatc acgggggtga tcagcaggtc ctccgggcgg gtatccatgg
    26401 tgcactggta aatgtagacc tcggcgtcca tcgcgcgggc gacggcgtag gccagggtcg
    26461 tcttgccgac g
//
LOCUS       DAOPCO010000028        26229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2026_length_26229_cov_5.574196, whole genome shotgun sequence.
ACCESSION   DAOPCO010000028 DAOPCO010000000
VERSION     DAOPCO010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 26229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26229
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2026_length_26229_cov_5.574196"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLS90_03855"
     CDS             <1..275
                     /locus_tag="PLS90_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018618091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HPK01571.1"
                     /translation="MDGAWLGSIDISQKKARTARFFDMNTGEIGKLSQPGGSLYNIEV
                     SNGGLITFPGGIPLKIGDTVVGGIGVSGSTVENDHEVASAGAAALK"
     gene            complement(369..866)
                     /locus_tag="PLS90_03860"
     CDS             complement(369..866)
                     /locus_tag="PLS90_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK01572.1"
                     /translation="MSIESLRDLFVDEMKDIYHAEKQLVKALPKMARAAATDELRAAL
                     EAHLDETKGQVERLEEAFALLELPRRGKKCEAMEGLVSEGEELIEEEAEPIVKDAGLI
                     ASAQKVEHYEMASYGTLVSFAKLLKENKVATLLEQTLAEEKNADRKLTAIAEKYINVE
                     ALGGA"
     gene            865..1425
                     /locus_tag="PLS90_03865"
     CDS             865..1425
                     /locus_tag="PLS90_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01573.1"
                     /translation="MAELLLDKNKSLVFFMCATVMSRRTIAGEQFMCQPPRTRRSSGA
                     GGENVKNRAAKQREAWREGRSTKFEFRNPKLIQTPEFQKQKNARNIYLRKVQQYSRSD
                     TAGLDSGKQPRGKTEISALFARFHDVKCFQGGAGFGKQRREAAIKGDESTTAMHGHPQ
                     QVSVGHLAVANKKLGGNTFIKTSGAS"
     gene            complement(1428..2390)
                     /locus_tag="PLS90_03870"
     CDS             complement(1428..2390)
                     /locus_tag="PLS90_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK01574.1"
                     /translation="MPRPARFKKRRLRRRLRPLTDRLLYGLVEGLRRVLARLPHALVL
                     ALGRAAGSLGYLVCGGLRRLAMRQLRETGIAPDEREARRLARATFRSLGMNALEWLHS
                     SGWDRPRLEACVRVEGIEHIREGERAGRGIIMVAYHFGNWELLSSSFRLVIDYPALVV
                     MTPIRNPLLNDFVVRMRQRWGFQMVTQNDVRTILRGLRAGWMLAVLADQDTRRVRGIH
                     VDFLGRPALTPSGAAVIAWRTGAPICPYTIERTAEDPRRHILRGHPCIWPDPDAPEEQ
                     EVRRLTQAYTDVLAREIRRRPDQWVWIHDRWHHPPRRHHDALAS"
     gene            complement(2552..3400)
                     /locus_tag="PLS90_03875"
     CDS             complement(2552..3400)
                     /locus_tag="PLS90_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01575.1"
                     /translation="MNSPITNSLAANEPLGATAARLGFVTPEQVIEALDEQRRRRAQG
                     ENRRLGLLMIEMGLLNAAQLDEVLRQYEALRPAVRDEACHLAVRLRAGLADQDRTILF
                     TSAGPGEGVTSTAYQVGLGLALMDQGHVLIVDANYRTPALEKMVRRYNPSHRDASVAV
                     PGLSELIRGEAEFVDALFPTPLRALSLLSTGSTDVDFLALMLSEHCSRVLRVLRENFR
                     FILIDAPPILRYPDAALLATRADAAVMVVAAGRCRRGQVDEMRRSLEMMRVPLLASVL
                     VEGDSR"
     gene            complement(3400..4905)
                     /locus_tag="PLS90_03880"
     CDS             complement(3400..4905)
                     /locus_tag="PLS90_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPK01576.1"
                     /translation="MSSAPVAGRGRLAWLTAGARALRRRKRRVILSTLILALLAWILA
                     QSLPPCYRSEARLLVQPVGTPPAVFQATGLAGVGQTDVRTQMVLLQSKYLLHRVAERL
                     GAAELQTASPPPANLLQAIRYYARRGVAGADDAWYKLMAALGLAEAETEADRALRKLQ
                     RGLHVSQENGSALIRVGLCLARPELAREALDLLIAEQARIQAGLATDEEELVPHLLEE
                     ELEQSRRRLSASDREWEETRAQYGIISLEEQRALLLDRLHEIERERDATERELELARV
                     NREVLDADGAAPAEPPLLADPTLDLVKRRIAQLQIDRINLLNLYTPASTQVRRLDQEI
                     VRLETMLREGLEARARTLADQAARIEEQLAQLETGESRQAEAGRERRLAEAEFLTFSQ
                     FLTGNRLQDAIGHAAVRLSLVEGPSLPTVPAWPPKRTIVGWGAALGLLLGLGLALMAE
                     RRADWILTARDLAEIPDVLFLGSIKLGTQFNDFEDKAHAAPAAIRPERETA"
     assembly_gap    5123..5222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6032..6547)
                     /locus_tag="PLS90_03885"
     CDS             complement(6032..6547)
                     /locus_tag="PLS90_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01577.1"
                     /translation="MSRPIPNDPRLPPTVLPDERRHVPATPADPTLAPDGQARLPEDL
                     WADYEQLRLPRSESLRSIRGLRTVGEQRGERRPRRSPWWTYPVVYLMFVLASTLFIGL
                     VLPFYLPVFAMPVLVLLIPLLLVLGGILGAIKGFKWLREAREWEREQERLHRQREGPI
                     DHLLADVKEKE"
     gene            complement(6650..7798)
                     /gene="hemW"
                     /locus_tag="PLS90_03890"
     CDS             complement(6650..7798)
                     /gene="hemW"
                     /locus_tag="PLS90_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPK01578.1"
                     /translation="MDRPAGIYVHWPFCERKCPYCDFYTFGREHPWQRLADDYLAALL
                     AEIRDAPRRFGWPDPPAADTIYFGGGTPSLMGAGPLGRLLAALGEVFRFSPAAEITLE
                     VNPTTVETAGLADLLAAGVNRLSVGCQSFNDRLLAELGRVHDAATTRRALEHIRALGP
                     VNLSLDLIFGAPTQTTADLEADLEAILGFEPDHVSAYNLTVHEGTPYARRQREQRLPL
                     PPEETQAAMFETIIARLGAAGLEHYEISNWARPGRHSRHNSKYWRPVDVFAFGVSAHG
                     VTRGRRYEHPRDLRAYLAAPTESVAWCEPPADARARAGEILMLALRRVGGTPWAEIDA
                     WAGADLRRYYRTELERLERDGLLRWREGRLALTRRGLLLADTVAEAFF"
     gene            8279..11044
                     /locus_tag="PLS90_03895"
     CDS             8279..11044
                     /locus_tag="PLS90_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01579.1"
                     /translation="MSCRPSRRRALLACLGLILPLAFAAPAAAQSPVGLNRLQEGTSV
                     VEQLDQFYVDGAGPFIAARAGDWRLANDRISLTFAGVGDLPATISLPVAGYRWNVDED
                     NRLPGALIDVATADMSLDFLGAFTQGIGLNGTDPILRYDTIEPVRGEGEVGLRLEGSP
                     FETALVRLETIYWLAGGSSQVRLESRFLDLPADEPLPPLVDVTNWGAASMLAESLGPP
                     KPGRVHRRAVEFYLGYWGELALGVTAPAGELDGVFVLRPSVSRVLAAPLPPEAAPQRG
                     YESYLADAFTTATLPFDPMELDARWWEPAPNDRTLVRHLFVVHGAPNELVEAIQAFRG
                     VETGAYEGQVTAADENGQPTPVADAEIRFTRLYQEDPGQRLRITAEEVYTIARTGADG
                     RFRVEVPPGHYIPRAEVETRVNRGTLTSGRLEPGETQEVNFTLPGRSGIRVRAIDAAT
                     SAPLAARLRIESTPPAPPVDLGPPLRARGYYRTAYIPPAGGEIYLPNGRYYVAASHGI
                     EYHRNEIEVWVENGKVASHVIPLDRASPTDGWTHVEIGARTDQTPGVEVTADDLVLMA
                     AAEGLDWVITGDFEHLTDLTPALARLGLEGQLGLSRGFRTMLPARPDWGQFLVYPVAA
                     DAPDPAVAREAWADLTTAAEFIAALRRLYPGALIQCDLIYTKEGRGYFGEPNRNPYEI
                     SFTPKPEVETAIDAITLVRPRDPNQVMKDLIGFWTNHLITGRFYLPASSASGRIPLGA
                     EPGYPRLLVHTGAAQDQLPTEAELFAAMRAHRVQLTTGPFVEYALGDAGPGGLAPHDP
                     AMAARTRVTAPNWADVTMARYAKEGNLQEFRQFHGNPEIGQRYPDPATGQRYAERTQK
                     ELGIRTDKDTLLNADTYAERTLGDYLPHYNLTGDTPSFALTFPIIIDADGDGVYDPLE
                     LYRQKGM"
     gene            11394..12986
                     /locus_tag="PLS90_03900"
     CDS             11394..12986
                     /locus_tag="PLS90_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01580.1"
                     /translation="MTPRRIILVTVLLVGVAGGALLFTPPVQRHLRTNRVLYLRNAHA
                     YAEALESFRELKRRVPELPRNLQAFERETVEHASAYFLRDGGMLSDADRARYAAFQAA
                     VREEGDLAALGRRLELQLAARRDGPSTATLAAARAVLERDGYDLEALWWAVRSQYDPC
                     RPLTLPAELVAWRDPLEARAPFDPLHPDRQPSAQQQQRKVYLMALLALADRDWASAAA
                     RFEEYARLDSREEEARSTAGDELPQALALLRSGQPAAAAYLLQREASEPTAAALLVEA
                     WLALGEPDRAAMRYDAARAIDAAGAERLLTEYLGLAAAAGNDPLLTLAHSNLRTHADG
                     LMLWRFAERASDGDSPAARALAQTAAGWLEPGASETEAIQIAQFALQRDQPELARRAA
                     AAVRSVPLGRSLQRLIASRADGATSGPRVAQDLSLLLTRNATRHFDLNLPPEAELVVI
                     NLQGYPADGVWPLVHVELESLGGVAHYARDSRARAQPLLIRPRRIGPATPGPRPVTVS
                     VSLLNGDDAGRRNVMVSGAQVF"
     gene            13061..15565
                     /locus_tag="PLS90_03905"
     CDS             13061..15565
                     /locus_tag="PLS90_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01581.1"
                     /translation="MDNQSSQEPRSDPPEQPEAETGPPAPPAAAETPMPAAADPAPTP
                     EPTPGPRRRRRWLSAAGAHLPLLIPLAVLIILRWLLVHPDPTDYPLDRNITMALRMQV
                     MRRDIRELDLRLERNKNQLARVSSATPEFPFIPIGSWLLGRFGVDVVVAARLMSLAAA
                     IATGLGIYLLGARIGPLATAQVALWLYALLPLAVLMGRSMLPDTLMVAAIVWAMAAAG
                     AGRGRVNLAMALPMGVLLLLAGLAKLPAVFFAPAAAWIYLAAAGWRRPTAWITLVFIT
                     LMVYAGICGWYRIDPRDPLVGLERISRGTNDALASAAEVLTAEAMMVMLGRVVLALTL
                     PGALLLLFAWVFLQGRPGHRAWLNLWLVVSLAFVLITVKANSYWAYAVVPVACLTIGA
                     GLARLWRAGRWMLVPLALVAAAAGVIDPAREQIRAYLATRPAYQVAGREVPGLAKRGR
                     LLYDGQVPDDLFFFVRRPGQSLQNRARQAAESMLNSDYYQYFLTTDVAGSALTDWQFD
                     TRPLIRFHPAAYALFQMGRATTIGESVAADEITSGAALNLHDRLLVLEATVDPARPEP
                     GRHMELSVVFQKGPEFHPGLILAGRLLHEPSGEELPLIAEAGGADFWTWRQPQLQVPE
                     FPVTDVMRVRYDIRALPHLPAGPYDLILQLLRDRRQHAPDGEAIRVPLDLELRPVQPK
                     LPLTIDVEDVLWRRRQAVVGPAWRGKPVVRWRLGSESDVWIAPGLPPGRYDLTVTAAG
                     EPAGAEPGDRRWPVLGVKQPGRSEQTLRFDSRRPRTLHLRFELRGPEDFLHLSLNNPR
                     YDDSPRVALPLYLIDFARGDRALLIESIQIDLAAQP"
     gene            15562..17457
                     /locus_tag="PLS90_03910"
     CDS             15562..17457
                     /locus_tag="PLS90_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01582.1"
                     /translation="MTGAPAPRPGAILTVVSFLLAAWIAFSFGFTPLRTSHDEWWHLK
                     TGAWLSRHGLPETDIFTYTAADIPWHNHEWLTQLGMWGLYRAGEISGFGGWRLVILVK
                     SLTIVAAFVGLALWLGRRRPDAALYALTGALLAALARRTFYPRPPFVSYLLLAIVLAV
                     LIGARGGRRGRAPWGLPPLFALWANLHGGWMAGLVVVGAFWADAAAHWALAAARQGPR
                     AVRRRRGAALRRLTLLGLLCALATLVNPYHVHLYALAGRVMNDPYLLPRIGEMLPPDW
                     HYVWILEGVILLLLFVGVRPVRPPAWIGAALAAAALHCLLRATANPWLQTALALPVIA
                     LALARRPRRPGWLAFLLLACFFGQQGIHHVRHLSLLAVVLLPPLAWGLACWARAWRGR
                     AGARRPTELTRLTPALIALLALALLGGYWSFSHRERASYLERNLMLARGSEWEPQWID
                     ARARETPVQSTLPAGVFLVEPYPRKAVDFLLRAGLPAPLWNGGNYAGYLIWRLAPETM
                     RVFTDNRYDVYGGRFVKQEHSVLEGLTQAGMEAAPAGEAWLREAGFMPWNEVLDRWGV
                     QTVFVPVESEVNARLEEAGWARVWEDFSFAIWVRPTAENQPIIERAKTLPRPNPWLAV
                     AEGREGT"
     gene            17629..17934
                     /locus_tag="PLS90_03915"
     CDS             17629..17934
                     /locus_tag="PLS90_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01583.1"
                     /translation="MNLAVAAASPRFAAFVALNWASTHAGGSGTDRMWHPRPSDPPVA
                     EIIDVEAQPVAPTRLTGEVASELEAPARMERYAPLRGRGARPVYAAPLPGRRLSLFA"
     gene            complement(17998..18546)
                     /locus_tag="PLS90_03920"
     CDS             complement(17998..18546)
                     /locus_tag="PLS90_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01584.1"
                     /translation="METGTEPRADLSAASLDPPPARTFPEPSLTLVEFGDALGVLVAA
                     RTERDTDHRRIVLKKVGYIRLEPGGWGGTAYPEIHILQNAEGLFVAVEPRTNKILRHP
                     SRRRYEIAFIGKDLFDCAIQCLRERDAEPLAELIAVTFAGRDGFSVEHGCLVAPDDYA
                     AERTAVSEDGLLPTPPRFDQNP"
     gene            complement(19007..19390)
                     /locus_tag="PLS90_03925"
     CDS             complement(19007..19390)
                     /locus_tag="PLS90_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPK01585.1"
                     /translation="MNIYVGNLSYQTTEPELRELFEQFGEVASVSIIRDKMTDRSKGF
                     GFVEMNNEEEGNAAIAALNEKEVKGRALRINEARPREDRPARGPRTSGSGFGGPRREG
                     GFGGPRREGGFGGGRRDGGNGPRGW"
     gene            complement(19809..20240)
                     /locus_tag="PLS90_03930"
     CDS             complement(19809..20240)
                     /locus_tag="PLS90_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPK01586.1"
                     /translation="MDLGTSDPKTAIRELARLACAGTEVDPEVAAEAVWRRERAMSTS
                     LDHGVAVPHARIDGLKSPQMAIGISPTGVDFDSMDGRPTHLIFLMLTPRDDQRIQLDL
                     LADIGRTFRDPVLVERCSQSRNFTEFLALIRSHLGERHASE"
     gene            complement(20292..21521)
                     /locus_tag="PLS90_03935"
                     /pseudo
     CDS             complement(20292..21521)
                     /locus_tag="PLS90_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010854661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
     gene            complement(21518..22525)
                     /locus_tag="PLS90_03940"
     CDS             complement(21518..22525)
                     /locus_tag="PLS90_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPK01587.1"
                     /translation="MRGASTADHGRSRDPAYRFPASSLATDHLREAPSVCPLPSSPGE
                     AAPAPLRVLACVADSPHAGVIVSEAAAIARTLSAELELLHVAPAAEATQARLQAILRD
                     HGLEPGAYPLAVRDGRPAHVIRAQARQGLIDLIVMGANEREGLLDSLMMGSIARRVAL
                     TAPCSVLLLSQGQAPARTGFREVVVATRLDDYSRQMLALISRTARQVGTQTLHVVREL
                     SVLELRALEYDIGSPRELEQHQRRLLFEMNEALLNFIEPIDFGELKLRVHCLEAGEGV
                     GAMDYARGHGADLLVYPAPPRPLSLLHRFFDNRRSVVLQQIPCALLFYRPSPHRPRVA
                     P"
     gene            22755..24161
                     /gene="radC"
                     /locus_tag="PLS90_03945"
     CDS             22755..24161
                     /gene="radC"
                     /locus_tag="PLS90_03945"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPK01588.1"
                     /translation="MPSDYQLIDGFERLRDACQRVNPGVGAVPAAPEADEPAALRVAL
                     AWLRALAPARDAAPPERRRLRRLARILTGEADDEVHEAEPRDERLLARRAAWRAGLDR
                     AQRRRLWPLADDAPDRSLAEEAAAWAEALPGLTGVRPWRFLRGLGRPVIEPEMPLRRF
                     LWRLGLLERADGDDAHLRRAHGELERIVQLTGLPAEVLRCLLRWHTGGERELTGGRVC
                     GARPRCAGCPFGELCPFARYKAAPAPDADGAAEALAEAGRRLREGRAAELGEADLLAL
                     TLAAGGMPRRAATRAAAELLRQYGNLRTLSQATPPQLAESPGLTPARCALLLAALELG
                     RRLGALALQPGSPITCSEEVWQAYRLRYRDLAQEHFVVLLLDAKNRVIHEHVVSKGTL
                     TGSHAHPREVFQQAVRRSAAAMILLHNHPSGDPTPSPEDRAVTARLRQAGEILGIRVL
                     DHIILGAEQYYSFRDRED"
     gene            complement(24230..25084)
                     /locus_tag="PLS90_03950"
     CDS             complement(24230..25084)
                     /locus_tag="PLS90_03950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01589.1"
                     /translation="MPFLTLISRWFAPAETRRLVRRKKPSRTRGLIEVRRRIGDLRPL
                     IDARLGQGRPVRILEVGCGFGAALMDLRKLYGDQVELHGINRYEHHGNWRRIRRTAVR
                     MGLFSDEESRAVTPPAVAFYDAGEGLRHETGYFDLVYSQASVFMVPDKIRLLEEISRV
                     LRPDGIARIDVWVRRKRFPKPYDYVIIIREGDREIPFWDYIKRFPTMKRRRTQRQGLK
                     WTRWLNRLRKPLGLPRIPHPKKKPYLEMKPHPALDLGLRLVETTNLNDLNPDWYGVQS
                     VYEVRGDG"
     gene            25284..25937
                     /locus_tag="PLS90_03955"
     CDS             25284..25937
                     /locus_tag="PLS90_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01590.1"
                     /translation="MSTATPPIPAWLFRALERLGEPSRGGPRRAGLGGQVTAAAMLLL
                     FALPGSLVGIAMIRFWNRDAAPRCFHALYDSGLMLPLGLAAVHLPLAWLLLHGRLRAT
                     PAVLGEVERLQPLAPGRRWLELSAPRLAVAGALAAGLVFVLALSEVHASILLAAPGQE
                     TLAIRSLTLLHYAPDRLVAAFCLVQLATTAAGLALLAAASVAGLKLWRRLAPHHAVD"
     gene            25924..26103
                     /locus_tag="PLS90_03960"
     CDS             25924..26103
                     /locus_tag="PLS90_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK01591.1"
                     /translation="MPLIECRNLQRRFGRPPHEVEALAGVSLAVDAGQWVWVVGPSAS
                     GKTTLLRARAGRIEP"
BASE COUNT         4008 a         8941 c         9098 g         4082 t
ORIGIN      
        1 ggatggacgg cgcctggctg ggcagcatcg acatctcgca gaaaaaagcg cggacggcgc
       61 ggttcttcga catgaacacc ggcgagatcg gcaaactgag ccagccgggc ggctcgctct
      121 acaatattga agtctccaac ggcggactga tcacgttccc cggcggcatt ccgctgaaga
      181 tcggcgatac ggtggtcggc gggatcgggg tgtcggggtc gaccgtcgag aacgaccacg
      241 aagtggcgag cgccggcgcg gccgcgttga aataaggccc gtcgcgcggc caatacgacg
      301 agccccgggc gacatcgccc ggggttcgtt tacattcgtc cctcgtcctc ttcctcgtcc
      361 tcgatctgct acgctccgcc caatgcttcc acattgatgt atttctcggc gatcgcggtc
      421 agcttgcggt ccgcgttttt ctcctcggcc agcgtctgct cgagcagcgt cgccaccttg
      481 ttctccttga gcagcttcgc gaacgaaacc agcgtgccgt agctcgccat ctcgtaatgt
      541 tcgaccttct gcgccgaggc gatcagtccg gcatccttca cgatcggctc ggcttcctcc
      601 tcgatcagct cctcgccctc ggagacgagg ccttccatcg cttcgcactt cttgccgcgc
      661 ctgggcagtt cgagcagtgc aaaggcttcc tcgagccgtt ccacctgacc cttcgtctcg
      721 tccaggtgcg cttcgagggc tgcccgcaat tcatcggtgg cggccgcgcg cgccatcttc
      781 ggcagcgcct tcacaagctg cttttcagcg tggtagatgt ccttcatttc gtcgacaaac
      841 aaatcgcgca atgactcaat gctcatggcg gaactcctcc tggataaaaa caagtcgctt
      901 gtttttttca tgtgcgcgac ggtcatgtcg cgacgcacga ttgcggggga gcaattcatg
      961 tgccagccgc cgagaacaag gagaagctca ggcgcgggtg gagaaaacgt caagaatcgg
     1021 gccgcaaagc agcgagaggc ctggagagag ggaagaagca cgaagttcga atttcgaaat
     1081 ccgaaactta ttcagacccc tgaatttcaa aaacaaaaga atgcacggaa tatctacctt
     1141 aggaaggtgc agcagtactc cagatccgat accgctggcc tggactctgg aaagcagccc
     1201 cgcggtaaga cggaaatctc agccttattc gcgcgtttcc acgatgtgaa gtgctttcag
     1261 ggcggcgctg gatttggaaa gcagcggcgc gaggccgcga tcaagggtga cgagtcgacc
     1321 accgcaatgc acggccatcc ccagcaagta agcgtcggtc acttggcggt ggccaacaag
     1381 aagctcggag ggaatacgtt catcaagacc agcggagcaa gttgatgtca agatgccagc
     1441 gcatcgtgat gacgcctcgg cgggtgatgc cagcggtcgt ggatccagac ccactgatcg
     1501 ggccggcggc ggatctcgcg cgcgaggacg tcggtgtagg cctgcgtcag ccggcggacc
     1561 tcctgctcct cgggggcgtc ggggtcgggc cagatgcacg gatggccgcg caggatgtgg
     1621 cgccgcgggt cctcggccgt ccgctcgatc gtgtaggggc agatcggcgc gccggtgcgc
     1681 caggcgatga cggccgcgcc cgacggcgtc aacgccggcc gccccagaaa gtcgacatgg
     1741 attccccgca cgcggcgcgt gtcctggtcg gcgagcacgg ccagcatcca gccggcgcgg
     1801 aggccgcgca agatcgtccg cacgtcgttt tgcgtgacca tctggaagcc ccaccgctgg
     1861 cgcatgcgca cgacgaagtc attgagcagc ggattgcgga tcggcgtcat gacgaccagc
     1921 gccgggtaat cgatcacgag ccggaaactg gaggagagca gctcccagtt gccgaagtgg
     1981 taggcgacca tgatgatccc gcggccggcc cgctcccctt cgcggatgtg ctcgatcccc
     2041 tcaacgcgga cgcacgcttc cagccgcggg cggtcccagc cggacgaatg cagccattcg
     2101 agcgcgttca tgccgagcga acggaatgtc gcgcgcgcca gccgccgcgc ttcccgttcg
     2161 tcgggcgcga tgccggtctc gcgaagctgg cgcatggcca gccgccgcag cccgccgcat
     2221 acgagatagc ccagcgagcc cgccgcgcgg cccagcgcca ggacgagcgc gtgcggcagc
     2281 cgcgcgagca cccggcgcag cccctcgacc agcccgtaga gcagccggtc ggtgagcggc
     2341 cgcaggcggc gccgcagacg gcgttttttg aatcgggcgg ggcggggcat caggcgcgac
     2401 ccttgccgtt gaatgatcgc cggggccctg ggagcagggc gcgcggcgtt tcctacattc
     2461 aacacgccgc ctgattttgt aaagggtcat caaaatcggt cggagcgggc ggatacgccg
     2521 gatcggtcgg agcatgcggg gacgcgcgcg ctcagcgcga atcgccttcg accagcaccg
     2581 acgccagcag cgggacgcgc atcatctcga gggagcgccg catctcgtcc acctggccgc
     2641 gccggcaacg ccccgccgcg acgaccatga cggcggcatc ggcccgcgtc gccagcagcg
     2701 ccgcgtcggg atagcgcagg atcggcggcg cgtcgatcag gatgaagcgg aaattctcgc
     2761 gcagcacgcg cagcacccgc gagcagtgct ccgagagcat cagcgccagg aaatccacgt
     2821 cggtcgatcc ggtcgacagc agggagagcg cgcggagcgg ggtggggaac agggcgtcga
     2881 cgaattccgc ctcgccgcgg atcagttcgc ttagccccgg gacggcaacg ctcgcgtcgc
     2941 ggtgcgacgg attgtagcgc cggaccatct tttccagcgc cggcgtccgg taattcgcgt
     3001 cgacgatcag cacgtgcccc tgatccatca gcgccagccc cagcccgacc tgatacgccg
     3061 tcgacgtgac gccttcgccc gggccggcgc tggtgaacag gatcgtgcgg tcctggtcgg
     3121 ccagaccggc gcgcaggcgg accgccagat ggcatgcctc gtcgcgcacg gcgggccgca
     3181 gcgcctcgta ctggcgcaag acctcatcca actgggccgc gttgagcagc cccatttcga
     3241 tcatcagcag gccgagccgg cggttctctc cctgcgcgcg gcgacggcgc tgttcgtcga
     3301 gcgcctcgat cacctgctcg ggcgtgacga aaccgagccg cgccgcggtg gcgcccagcg
     3361 gttcgttcgc cgccagcgag ttggtgatgg gtgaattcat caggccgtct ccctctcggg
     3421 gcggatcgcc gccggcgcgg cgtgggcctt atcctcaaaa tcgttgaatt gggttcccag
     3481 cttgatcgac ccgaggaaga ggacatcggg gatctcggcc aagtcgcgcg ccgtcaggat
     3541 ccagtcagcc cggcgttcgg ccatgagcgc cagcccgagc cccagcagca ggccgagcgc
     3601 cgcaccccag ccgacgatcg tccgcttggg cggccacgcc ggcacggtcg gcagactcgg
     3661 tccctccacc agactgagcc ggacggccgc gtgcccgatg gcatcctgca gccgattgcc
     3721 ggtcagaaac tgcgaaaagg tgaggaactc cgcctccgcc agacgccgtt cgcggccggc
     3781 ctcggcctgg cgcgattccc cggtctcgag ctgcgccagt tgctcctcga ttcgcgccgc
     3841 ctggtccgcc aacgtcctgg cccgtgcttc gagcccttcg cgcagcatcg tctccagccg
     3901 cacgatctcc tggtccagcc tgcgcacctg cgtggaagcg ggggtataga gattcagcag
     3961 gttgatccga tcgatctgaa gctgcgcgat ccggcgcttg acgagatcca gcgtcggatc
     4021 ggccagcagg gggggctccg ccggcgccgc gccatcggca tcgagcactt cgcgattgac
     4081 ccgcgccagt tccagttcgc gttcagtcgc gtcgcgctcg cgctcaattt catgcagccg
     4141 gtccagcagc agggcgcgct gctcttccag cgagatgatt ccgtattgcg cgcgtgtttc
     4201 ctcccattcg cgatccgaag cggagagccg ccggcgcgat tgctccagtt cctcctcaag
     4261 aaggtgggga acgagctcct cctcatcagt cgccaggccg gcctggatcc gcgcctgttc
     4321 ggcgatcagc agatcgagcg cctcgcgcgc cagctccggc cgcgccaggc agaggccgac
     4381 ccggatcaac gccgaaccgt tctcctgaga gacatgcagt ccgcgctgga gcttgcgcag
     4441 cgcgcggtcc gcttccgttt cggcctcggc cagccccagg gcggccatca gtttatacca
     4501 ggcatcatcg gccccggcca cgccgcggcg ggcgtagtag cggatcgcct ggagcagatt
     4561 ggcggggggc ggcgaggccg tttgcagctc ggccgcgccg agccgttccg cgacgcgatg
     4621 gagcagatat tttgactgga gcagcaccat ctgcgtgcgc acgtcggtct gaccgacgcc
     4681 ggccagtccc gtggcctgaa agaccgccgg cggcgttccc accggctgca ccagcagacg
     4741 cgcttcggaa cgataacagg gggggagcga ctgcgcgagg atccaggcaa gcagcgccag
     4801 gatcagggtg gagaggatga cgcgacgctt gcgccggcgt agcgcgcgcg cgccggccgt
     4861 cagccaggca agacggccgc gaccggcaac gggagcagag gacatcgcgt gcatgaggcc
     4921 tcgggagcag ggttccgcct gggcgcggaa ccgtgattga ccgtcaagat cttaacggcg
     4981 cccgcggagg caaacttgaa tttgaaatct tgagcggtgt tcacaagagg gaacagtcgc
     5041 cccccgttgc ccccatttcg cggggtcatg gagcatttta cgccccggcg ccagcgcgtc
     5101 aacggaacgt caggccggcg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnccctacgg gactcggtcg ctgggtctcc cggttcccgc cactgaagtg gcgggctaaa
     5281 gtctaccatc cctacgggac tcggtcgctg gatctcccgg ttcccgccac tgaaggggcg
     5341 ggctgaagtc taccgtccct acgggactgg gtcgctgggt ctcccgattc ccgccactga
     5401 agaggcgggc tgaaatctgc cgtccctacg ggactcggtc gctgggtctc ccggttcccg
     5461 ccactgaagt ggcgggctga agtctgccgt ccctacggga ctgggcggct gtggcggtcg
     5521 aggcgggcaa ggatctcgcc gaggactgcc gtccctacgg gactgggcgg ctgtcgctcc
     5581 gcaggcacgg cctggttgag ccgctgctac cgtccctacg ggactgggcg gctgagctcc
     5641 gcgccccacg gggacagaca gcaccgcctg ccgtccctgt cgggactggg gggctggtgg
     5701 gcccggcagg gcctcggcca ttcgaatatg tcgttcacgc ctgaggggac cggctggtaa
     5761 tcggacctgc cgggctttgg ccgttcgtag atgcgaccac cgcaattcgt tcccagttcc
     5821 aatctccgcg ccccggcgtc tgcgcgtgag atccgctgga actgcttcct gttccaacct
     5881 tcaagtcccg aatggcggcg ctgacgaaat cccctcgccc gatgtcaaat cctcctcagg
     5941 cggagacgcc gagacgcgga gaaaagtcca tgaattggct cacggatcgc ccggctaatt
     6001 tcgatcttgc gccagttaca ttccgcttcc ctcactcctt ctctttcacg tcggccagca
     6061 ggtggtcgat cgggccctcg cgctgccggt gcagtcgttc ctgttcccgt tcccactcac
     6121 gcgcttcgcg caaccacttg aatcccttga tcgccccgag gattcccccc agcaccagca
     6181 gcagcggaat cagcaggacg agcaccggca tggcgaacac cggcagataa aagggcagaa
     6241 ccagcccgat gaacagcgtg ctggcgagga cgaacatcag gtagacgacc ggataggtcc
     6301 accacgggct gcgccggggc cggcgttcgc cgcgttgttc gccgaccgtg cgcagcccgc
     6361 ggatcgaacg gagcgactcg ctgcgcggca ggcgcagttg ctcgtaatcc gcccacagat
     6421 cctcgggcag tcgggcctgg ccgtcgggcg ccagcgttgg gtcggccggc gtggcgggga
     6481 cgtgccggcg ctcgtcgggc aataccgtcg gcggcaggcg gggatcgttg ggaatcggtc
     6541 tggacacgct caggaccctc acggcgaacc cggtggacgc agcggacgtc ttccccttca
     6601 gtcgcctcgt ttgcccgccg ggttgcaaga caacgaccga tgccccgtct caaaagaacg
     6661 cctcggccac ggtgtcggcc agcagcaagc cccggcgcgt gagcgccagc cggccctcgc
     6721 gccaccggag cagcccgtcg cgctcgagcc gctccagttc cgtgcggtaa taccggcgaa
     6781 ggtcggcgcc ggcccaggcg tcgatctccg cccacggtgt gccgccgacg cgccgcagcg
     6841 ccagcatgag gatctcgccg gcgcgcgcgc gcgcgtcggc cggcggttcg caccacgcca
     6901 cggattcggt cggggcggcc agatacgccc gcagatcgcg cgggtgttca tagcgccgcc
     6961 cgcgcgtgac gccgtgggcc gagacgccga aggcgaagac gtcgaccggc cgccagtatt
     7021 tgctgttgtg ccggctgtgg cggcccggcc gcgcccagtt ggagatttca taatgctcca
     7081 acccggccgc gcccagccgc gcgatgatcg tttcgaacat cgcggcctgc gtctcttccg
     7141 gcggcaaggg caggcgctgc tcgcgctgcc gccgcgcata gggcgtccct tcgtgcaccg
     7201 tcaggttata ggccgaaacg tgatccggct cgaacccgag aatcgcttcc agatcggcct
     7261 ccagatcggc ggtcgtctgc gtcggcgcgc caaagatcag gtcgagcgac agattgacgg
     7321 gacccagcgc gcggatatgc tccagcgcgc ggcgcgtcgt cgccgcatcg tggacccgcc
     7381 ccagttcggc cagcagccgg tcgttgaacg actggcagcc gaccgagagc cggttgacgc
     7441 cggcggccag cagatcggcc aggccggcgg tttcgaccgt cgtcggattg acctccaggg
     7501 tgatctcggc ggcggggctg aaccggaaga cctcgccgag cgccgccaga aggcggccga
     7561 gcggcccggc gcccatgagg ctgggcgtgc cgccgccgaa atagatcgtg tcggcggcgg
     7621 ggggatccgg ccagccgaac cggcggggcg cgtcgcggat ctccgccagc agggccgcca
     7681 gatagtcgtc ggcgagccgc tgccacgggt gctcgcgccc gaacgtatag aagtcgcagt
     7741 acgggcactt gcgctcgcag aatggccaat gaacgtagat gccggcgggt cggtccatca
     7801 gatgagatca tacagcaaaa accgtccggc gtcgctcgct cgaaattcgt ccgcgaatct
     7861 cgggatcgcc ggattggttc tgccgtcccc atgggactcg gtcgcgctgg gtatcccggt
     7921 tcccgccact gaagaagcgg gctgaagtct gccgtccctg acgggacctg ccctgctgat
     7981 gtatccggca aggcctgggc cattcgagtc ggccgttcac cccccttttc atccctcgtt
     8041 gtgaaccgcc ggtagatgag caactcgtcc cggaacggga atggacgccg tggatgccat
     8101 ggacacgatg gaaggctgct ggcgccggcc ccgtaccgtt tgacgccact acaaaaaaca
     8161 gggaaactta gccatttgag gcgtgtctca gtccgggggc gattgcgtcg ccggacccga
     8221 tgccgcaccg tctgtctgga ccgccgcatc ccacactccg acccggaaga gagttgtcat
     8281 gagttgtcgt ccgagccgcc gtcgcgccct gctggcctgc ctgggcctga tcctgccatt
     8341 ggcgttcgcc gcccccgcgg cggcccaatc gcccgtcggc ctgaatcggc tccaggaagg
     8401 aacgtcggtc gtcgagcagc tcgatcaatt ttacgtcgat ggggctggtc cgtttatcgc
     8461 cgcgcgcgcg ggggactggc gcctcgccaa cgaccgcatc agcctgacat tcgccggcgt
     8521 cggcgacctg cccgccacca tctcgctgcc cgtggccggc taccgctgga acgtggatga
     8581 ggacaaccgg ctgcccggcg cgctgatcga cgtggccacg gccgacatga gcctggattt
     8641 cctcggcgcg ttcacccagg gcatcggcct gaacgggacc gatccgatcc tgcgctacga
     8701 cacgattgag ccggtccgcg gcgaggggga ggtcggcctg cggctggagg ggtcgccgtt
     8761 tgaaacggcg ctggtccggc tcgagacgat ctactggctg gccggcggca gttcgcaggt
     8821 ccggctggaa agccggttcc tggatctccc cgccgacgag ccgctgccgc cgctggtcga
     8881 cgtgaccaac tggggcgcgg cgtcgatgct ggccgaaagc ctcggcccgc ccaaacccgg
     8941 gcgcgtccac cgccgcgccg tcgaattcta cctgggctac tggggcgagc tggccctggg
     9001 cgtcacggcg ccggccggcg aactggacgg cgtcttcgtc ctgcgtcctt ccgtatcgcg
     9061 cgtcctggcg gcgccgctgc cccccgaggc cgctccccag cggggctatg aaagctacct
     9121 ggccgatgcc ttcaccaccg cgacgttgcc atttgacccg atggagctgg atgcgcgctg
     9181 gtgggagccg gcgcccaatg atcgcactct ggtgcggcat ctgttcgtcg tgcacggggc
     9241 gccgaatgag ctggtcgaag cgattcaggc gtttcgcggg gtcgaaaccg gagcctacga
     9301 agggcaggtg acggcggcgg acgagaatgg ccagccgacc cccgtcgccg acgcggagat
     9361 ccgcttcacg cgcctgtatc aggaagaccc gggccagcgg ctgcggatca cggccgagga
     9421 agtctatacg atcgcgcgga ccggcgccga cggccgcttc cgcgtcgagg tgccgccggg
     9481 gcactatatc ccccgggcgg aggtcgaaac gcgcgtcaac cgcggcacgc tgacctcggg
     9541 tcggctggaa ccgggcgaga cccaggaggt taatttcacg ctgccggggc gcagcgggat
     9601 ccgggtgcgg gcgatcgacg cggccacgtc cgcgccgctg gccgcgcggc tgcggatcga
     9661 gtcgacgccg ccggcgccgc cggtcgacct cggcccgccg ctgcgcgccc ggggttacta
     9721 ccgcaccgcc tacatccccc ccgccggggg cgagatttac ctgcccaacg gccggtacta
     9781 cgtcgccgcg tcgcacggca tcgagtatca ccgcaacgaa atcgaagtct gggtggagaa
     9841 cggcaaggtc gcctcgcacg tgatcccgct ggaccgggcg agtccgaccg acggctggac
     9901 ccacgtggag atcggcgccc gcaccgacca gacgcccggc gtggaagtca cggccgacga
     9961 cctggtcctg atggcggcgg ccgaaggcct ggactgggtc atcaccggcg acttcgaaca
    10021 tctgaccgac ctgacgccgg cgctggcgcg cctcgggctg gaagggcaac tgggcctcag
    10081 ccgcggcttc cggacgatgc tccccgcgcg ccccgactgg ggtcagttcc tcgtctatcc
    10141 ggtggcggcc gacgcccccg acccggccgt agcgcgcgag gcctgggccg acctgaccac
    10201 ggccgccgaa ttcatcgccg cgttgcgccg gctctatccc ggcgcgctga tccagtgcga
    10261 cctcatttat accaaagagg ggcggggcta tttcggcgaa cccaaccgca atccctatga
    10321 gatctcgttc actcccaagc cggaggttga gacggcgatc gacgccatca cgctcgtccg
    10381 gccgcgcgat cccaatcagg tgatgaagga cctgatcggc ttctggacga atcatctgat
    10441 tacgggccgg ttctatctgc ccgccagctc cgcgtccggc cggattccgc tgggcgccga
    10501 gccgggctac ccgcgcctgc tggtccacac cggcgcggcg caggaccagt tgccaaccga
    10561 agccgaactg ttcgccgcga tgcgcgcgca ccgcgtccaa ctgaccaccg gcccgttcgt
    10621 ggaatacgcg ctgggcgacg cgggccccgg cgggctggcg ccccacgacc ccgccatggc
    10681 cgcccggaca cgggtgacgg cccccaactg ggccgacgtc accatggcgc gctacgccaa
    10741 ggaggggaac ctccaggagt tccgccagtt ccacggcaat cccgaaatcg gccagcgcta
    10801 ccccgacccc gccaccggcc agcggtacgc ggaacggaca cagaaggaac tggggatccg
    10861 gaccgacaag gacacgctgc tcaatgccga cacctacgcc gagcggacgc tgggcgatta
    10921 cctgccccac tacaacctga cgggcgacac cccctcgttc gcgttgacgt tcccgatcat
    10981 catcgatgcc gacggcgacg gggtgtacga cccgctggaa ctctatcggc agaaggggat
    11041 gtagcgggcc ccggttgaat tcgtcgctcg tccctcgtcc gtcgttgtga tcggggattg
    11101 gaagggagat gagggacgta agggacacaa attcatggcc cttgttgggt gattctggca
    11161 tccccgcggg atgcgggcgg ggggtggatc gattgcgatt gcgatagcga tcccgattgc
    11221 gataccgaga gcgagagctg ccaacttccc tgggcttgcc gccagggatc ttgcgcagat
    11281 cgttcgtgat cccctgtctt gccagcgggg ggccgctcgg ttaaggtctg atccatgggc
    11341 gcgcttactc cacttccgcc ggccgccagc cgccgccgac cggggccccg ccggtgacgc
    11401 ctcgccggat catcctggtg acggtgctgc tggtcggggt ggcgggaggc gcgttgctgt
    11461 tcacgccgcc cgtccagcgc cacctgcgca cgaaccgcgt gctttatttg cgcaatgcgc
    11521 acgcctacgc cgaggcgctg gagagctttc gcgaactcaa gcgccgcgtt ccggaactgc
    11581 cgcggaacct gcaagccttc gagcgcgaaa cggtggaaca tgcgtctgcg tacttcctgc
    11641 gcgacggcgg gatgctgagc gacgccgatc gcgcgcgcta cgcggccttt caggccgccg
    11701 tgcgcgagga aggggacctc gccgcgctgg gccgccggct ggaactgcaa ctggcggcgc
    11761 gccgcgacgg gccatccacc gccacgctgg cggccgcccg cgccgtgctg gagcgcgacg
    11821 gctatgatct ggaagccctc tggtgggccg tgcggagcca gtatgacccg tgccggccgc
    11881 tgacgctgcc ggctgaactg gtggcgtggc gcgatccgct ggaggcgcgc gcgccgttcg
    11941 atccgctcca cccggaccgc caaccgtcgg cgcagcagca acagcgcaag gtctacctga
    12001 tggcgctgct ggcgctggcc gaccgcgact gggcaagcgc cgccgcgcgg ttcgaggagt
    12061 atgcccgact cgattcgcgt gaggaggagg cgcggtcgac ggccggcgac gagctgccgc
    12121 aggccctcgc gctgctccgt tcgggtcagc ccgcggccgc cgcctatctg ctccagcgcg
    12181 aggccagcga accgaccgcc gccgcgctgc tggttgaggc ctggctggcg ctgggcgagc
    12241 cggaccgggc ggcaatgcgc tacgacgcgg cccgcgcgat cgacgcggcg ggcgccgagc
    12301 gcctgttgac cgaatacctg gggcttgccg ccgcggccgg aaacgacccg cttctgacgc
    12361 tggcgcactc caacttgaga acccatgccg acggcctgat gctgtggcgg ttcgccgaac
    12421 gcgcatcaga cggcgattcg cccgcggcgc gcgcgctggc gcagacggcc gcggggtggc
    12481 tcgagccggg cgcgtcggag acggaggcga tccagatcgc gcagttcgcg ctccagcgcg
    12541 accagccgga gctggcgcgc cgcgccgccg cggcggtccg gtcggtcccg ctcggccggt
    12601 cgctccagcg gctgatcgcg tcgcgcgccg acggcgcgac cagcggcccg cgcgtcgccc
    12661 aggacctgag cctgctgctg acgcgcaacg ccacgcggca ttttgacttg aacctgccgc
    12721 ccgaggccga actggtcgtc atcaatcttc agggttaccc cgccgacggg gtgtggccgc
    12781 tggtccacgt cgagctggaa tcgctggggg gcgttgcgca ttatgcccgc gactcgcgcg
    12841 cgcgcgcgca gccgctgctg atccggccgc gccggatcgg tccggccacg ccggggccgc
    12901 ggccagtgac ggtatcggtc tcgctgctca acggcgacga cgccggccgg cgcaacgtca
    12961 tggtcagcgg agcgcaggtg ttctaggatg agggattgcc cggatcaagc cggggccgcc
    13021 gcccgccgcc acgaggggcg cggtctcctt caggacagcc atggacaacc aatcgtccca
    13081 ggaaccccgg tctgacccgc ccgagcagcc ggaggcggag accggcccgc cggcgccgcc
    13141 ggccgcggcc gaaacgccga tgccggcggc ggccgatccg gcccccacgc ccgagccgac
    13201 ccccggcccc cgccgccgcc gacgctggct gtcggcggcg ggcgcgcacc tcccgctgct
    13261 gattccgctg gccgtgctga tcatcctgcg ctggctgctg gttcaccccg acccgaccga
    13321 ctacccgctg gaccgcaaca tcacgatggc gctgcggatg caggtaatgc gccgcgacat
    13381 ccgcgagctg gatctgcgcc tggaacgcaa caagaaccag ctggcgcggg tcagcagcgc
    13441 gacgccggag ttcccgttca tcccgatcgg gagctggctg ctggggcgct tcggcgtgga
    13501 tgtcgtggtg gcggcgcgcc tgatgtcgct ggccgcggcc atcgccacgg gcctggggat
    13561 ctatctgctc ggcgcgcgga tcggcccgct cgcgaccgcg caggtcgcgc tctggctcta
    13621 cgcgctgctc cccctggcgg tgctgatggg ccgcagcatg ctgcccgaca cgctgatggt
    13681 ggcggcgatc gtctgggcga tggccgcggc cggcgccggc cggggccgcg tgaacctcgc
    13741 catggcgctt ccgatggggg tgctgctgct gctggcgggg ctggcgaagc tgccggccgt
    13801 cttcttcgcg ccggcggcgg catggatcta cctggccgcg gcgggctggc gccggccgac
    13861 cgcctggatc acgctggtgt tcatcacgct gatggtctac gccggcatct gcggctggta
    13921 ccggatcgat ccgcgcgatc cgctggtcgg gctggaacgg atcagccggg gcacaaacga
    13981 cgcgctcgcc tccgccgccg aggtgctgac cgccgaggcg atgatggtca tgctggggcg
    14041 ggtcgtgctg gcgctgacgc tccccggcgc gctgttgctc ctgttcgcgt gggtcttcct
    14101 gcagggccgg cccgggcatc gcgcctggtt gaatctgtgg ttggttgtca gcctggcgtt
    14161 cgttctgatc accgtgaaag ccaacagtta ctgggcctat gcggtcgtgc cggtcgcctg
    14221 cctgacgatc ggcgccggtc tggcgcgttt gtggcgcgcc ggccgctgga tgctggttcc
    14281 gctggcgctg gtggcagcgg cagcgggcgt catcgacccg gcgcgcgagc agatccgcgc
    14341 ctacctggcc acgcggccgg cctaccaggt ggccgggcgg gaagtgcccg gcctggcgaa
    14401 gcgaggccgt ctgctctacg acggccaggt gcccgacgat ctgttctttt tcgtccgccg
    14461 gcccggccaa tcgctgcaga atcgggcgcg ccaggccgcc gagagcatgc tcaactcgga
    14521 ctactaccaa tactttctga cgaccgacgt ggccggttcg gcgctgaccg actggcagtt
    14581 cgacacgcgg ccgctaatcc gctttcaccc ggcggcctat gcgctgttcc agatgggacg
    14641 ggcgacgacg atcggcgagt cggtggcggc cgatgagatc accagcggcg ccgcgctcaa
    14701 tctgcacgac cggctgctgg ttctggaggc gacggtcgac ccggcacgac ccgaaccggg
    14761 ccggcacatg gaactgtcgg tcgtgttcca gaaagggccg gaattccacc ccggcctgat
    14821 tctcgccgga cgcctcctgc acgagccctc cggcgaggag ctgccgctga tcgccgaggc
    14881 gggcggcgcg gatttctgga cctggcgcca gccgcaactg caggtgcctg aatttcccgt
    14941 gaccgacgtg atgcgcgtcc gctacgacat tcgcgcgctg ccccatctgc cggccggacc
    15001 gtacgatctg atcctccagt tgctccgcga ccggcgccag cacgccccgg acggggaggc
    15061 gattcgcgtg ccgctggacc tggagctgcg gccggtgcaa cccaaactgc cgctgacgat
    15121 cgacgtcgag gatgtcctct ggcgcaggcg gcaggcggtc gtcggcccgg cctggcgcgg
    15181 caaaccggtc gtccgctggc ggctgggatc ggaaagcgac gtctggatcg cccccgggct
    15241 cccgccgggc cgttatgacc tgaccgtgac cgccgccggg gagcccgccg gggcggaacc
    15301 gggcgaccgg cgctggccgg ttctcggcgt caaacaaccc gggcggtccg agcagacgct
    15361 gcggtttgac tcgcgccggc cgcgaacgct gcacctccgc ttcgaactgc gcgggcctga
    15421 agactttctg cacctgagtc tgaataatcc gcgctacgac gactcgccgc gggttgccct
    15481 cccgctctac ctgatcgatt tcgcgcgcgg cgaccgggcc ctgttgatcg aatcgatcca
    15541 gatcgatctg gcggcgcagc catgaccggc gccccggcgc cgcgccccgg cgcgatcctc
    15601 acggtggttt cctttctgct ggcggcctgg atcgccttca gcttcgggtt tacgccgctg
    15661 cgcacgtcgc acgacgaatg gtggcatctg aagacgggcg cctggctgag ccgccatggc
    15721 ctgcccgaaa ccgacatctt cacctacacc gccgcggaca tcccctggca caaccacgag
    15781 tggctgacgc agcttggcat gtggggcctg tatcgggcag gcgagatctc cggcttcggc
    15841 ggctggcggc tcgtgatcct ggtcaagtcg ctgacgatcg tggcggcgtt cgtcggcctg
    15901 gcgctgtggc tcggccggcg gcgtcccgac gcggcgctgt atgcgctgac cggggcgctg
    15961 ctggcggcgc tggcccgccg cacgttttac ccccggcccc cgttcgtgag ctacctgttg
    16021 ctggcgatcg tcctcgccgt gctgatcggg gcgcgcggcg ggcggcgcgg ccgcgccccg
    16081 tggggcctgc cgccgctctt cgcactgtgg gccaacctgc acggggggtg gatggcgggg
    16141 ctcgtcgttg tgggggcgtt ctgggccgac gccgccgcgc actgggcgct cgccgccgcg
    16201 cggcaaggcc cgcgggccgt ccggcgccgg cggggggcgg ccctccgccg cctgaccctg
    16261 ctgggactgc tttgcgcgct cgcgacgctg gtcaatccgt atcacgtcca cctttacgcg
    16321 ctggccgggc gcgtcatgaa cgacccctac ctgctgccgc ggatcggtga gatgcttccc
    16381 cccgactggc actacgtctg gatccttgaa ggggtgatcc tgctgctgct gttcgtgggc
    16441 gtgcggccgg tccgtccgcc ggcgtggatc ggcgccgcgc tggcggccgc cgcgctgcac
    16501 tgcctgctgc gcgcgacggc caatccgtgg ctgcagaccg cgctggcatt gccggtgatc
    16561 gcgctggcgc tggcgcgccg cccgcgccgc cccggctggc tggcctttct cctgctcgcc
    16621 tgcttcttcg ggcagcaggg gatccatcac gtgcgccacc tctcgctgct ggcggtggtg
    16681 ctgctgccgc cgctggcgtg ggggctggcg tgctgggcgc gggcgtggcg cggacgggcg
    16741 ggcgcgcggc ggccgaccga actgacgcgc ctgacgcccg cgctgatcgc cttgctcgcg
    16801 ctggccctgc tcggcggtta ctggagcttc tcccatcgcg aacgcgcctc ctatctggag
    16861 cgcaatctga tgctggcgcg cgggagcgaa tgggagccgc agtggatcga cgcgcgcgcc
    16921 cgcgagacgc cggtccagtc gacgctgccg gcgggggtct tcctcgtcga gccgtatcca
    16981 cgcaaggcgg tcgatttcct gctgcgcgcc ggtctgcccg ctccgctgtg gaacggcggc
    17041 aactacgccg gctacctgat ctggcggctg gcccccgaaa cgatgcgggt cttcaccgac
    17101 aaccgctacg acgtctacgg cggccggttc gtcaagcagg agcattcggt tctggagggg
    17161 ttgacgcagg ccgggatgga ggccgccccg gccggcgagg cctggctgcg cgaggccggc
    17221 ttcatgccgt ggaacgaagt gctcgaccgc tggggggttc agaccgtgtt cgtgccggtg
    17281 gaatccgagg tcaacgcgcg gctggaggag gcgggctggg cgcgcgtgtg ggaagacttc
    17341 tcgttcgcga tctgggtccg cccgaccgcg gagaaccagc ccattatcga gcgggcaaag
    17401 acgctgcccc gccccaaccc ctggctggcc gtcgccgaag ggcgagaagg gacgtaacgg
    17461 atataaggaa cattaaaaac ttaagagact taaatgacct tgaactcgaa cctcggtgtc
    17521 aaataaaatt gacacgtata ggcggtatcg accagaatca acccggactt gagccggtgt
    17581 agcaaaaaat cgaacaccgc cgaacgaatc cggaatgctc cgttgcgcat gaatctcgcc
    17641 gtcgccgccg cctcgccccg tttcgccgcg ttcgtcgccc tgaactgggc gtcgacccac
    17701 gccggcggct cgggaaccga tcggatgtgg cacccccggc cgtccgatcc gcccgtggcg
    17761 gagatcatcg acgttgaggc ccagccggtt gcccccaccc gacttaccgg tgaggtcgcc
    17821 agtgagttgg aagcccccgc gcgcatggaa cgctacgcgc cgctgcgggg tcgcggcgca
    17881 cggccggtgt atgccgctcc gctgcccggc cgccgtctga gccttttcgc ctgatttccc
    17941 tcgcggcaac tggggcggca gcccctgaat gacatttacg tcccttttgc ccctggccta
    18001 tggattctga tcgaacctgg gcggtgtcgg cagcagcccg tcctcggaaa cggcggtccg
    18061 ctcggcggcg taatcgtcgg gcgcgaccag gcagccgtgc tcgacgctga agccatcgcg
    18121 gccggcgaac gtgaccgcga tcagttcggc gaggggctcg gcgtcgcgtt cgcgcaggca
    18181 ctggatcgcg cagtcgaaca ggtccttgcc gataaaggcg atttcatagc gccgccggct
    18241 ggggtggcgc aggatcttgt tcgtccgcgg ctcgacggcg acgaacaggc cctcggcatt
    18301 ctgcaggata tgaatctcgg ggtaggccgt cccgccccag ccgcccggct cgagccggat
    18361 ataacccacc ttcttgagca cgatccggcg gtggtcggtg tcgcgctcag tgcgggcggc
    18421 aacgagcacg cccaatgcgt cgccaaactc aacgagcgtg agcgacggct cgggaaaagt
    18481 gcgggcggga ggcgggtcca gggaagctgc ggagaggtct gcgcggggtt cggtcccggt
    18541 ttccatggga atgtcgtcct cgtcgccgcc gctggggaga ggggctgcaa gcggtgggtc
    18601 ccgcagacat acgacgggtt ccgctcgatc gccgcgggcg gcatctcatg tatctagttt
    18661 aggccaactg ggccgggctg tctcgccaaa agggaaacca cccggccgcg ggggaggttg
    18721 gaagccagca ttgtacccat cgccggggcc agggagagaa aggacagaag ggacgtaggg
    18781 gacgcaaggg acgtgtggaa cgtgtgggac ggatagcggc tatattcatc cgttcagggt
    18841 gacggcgaag gcgtcgtgaa agcctcggtg gacaatcagt cgcctatttg ttcgattccg
    18901 aaaccgattt aagagttcca tccccttgga aacgggccgg cgggatccct ccagatctcc
    18961 cgccggcccg cgcttgactc aatcgaaggc aactggaacg tgggcgctac cagccgcgcg
    19021 gcccgttgcc cccatcgcgg cgcccgccgc cgaaaccgcc ctcgcggcgc gggccgccga
    19081 agcccccttc gcgccggggg ccgccgaagc cgctcccgct ggtgcgcgga ccgcgcgccg
    19141 gccggtcctc gcgcgggcgc gcctcgttga tccgcagcgc gcggcccttg acctccttct
    19201 cgttgagcgc ggcgatcgcc gcgttgccct cctcctcgtt gttcatctcg acaaagccga
    19261 aacctttgga acgatccgtc atcttgtcgc gaatgatcga caccgaggcg acttctccga
    19321 actgctcgaa cagttcgcgc aactcgggct ccgtcgtctg gtaggaaagg ttcccgacat
    19381 aaatattcat ggactgtctc ccgtcatctc aatgccccgg acacaagcag gcccgcgggc
    19441 aggcattctc tgatcatccc catcgtttcg gccttggagg gtaagcggaa tcgtgggaga
    19501 gtacccgaga ttatgcacga accggtttcc aaatcaattc cggaaaaggg agacggccgc
    19561 ggggaggaca tgtgtcagaa ataacgggac cgggccactg aagtgtctcg tcattcccca
    19621 aattcagacc ggttccggcc accccttgcg gggattcgat tcgtcggggg ggcgacgcag
    19681 ggttccggcc cgcggccgtc acgctgcgct taattccggt cgtccactcc gcggactgaa
    19741 ccaatcaaaa gcaggaagcg cctgcttgaa tacggaatcc caaagcagct tgcgattagg
    19801 gaacccggct attcgctcgc gtggcgctcg cccaggtggc tgcggatcaa tgccaggaat
    19861 tcggtgaagt tgcgcgactg cgagcagcgc tcgacgagga ccgggtcgcg gaacgtgcgg
    19921 ccgatatcgg ccagcaggtc gagctgaatc cgctgatcgt cgcgcggggt cagcatcagg
    19981 aagatcaggt gggtgggacg gccgtccatc gaatcgaagt cgacgccggt cggcgagatg
    20041 ccgatcgcca tctggggcga tttcagcccg tcgatccgcg cgtgcgggac ggcgacgccg
    20101 tgatcgagcg aggtcgacat cgcccgttcg cggcgccaga ccgcctcggc cgcgacttcc
    20161 gggtcgacct ccgttccggc gcacgccagc ctggccagtt cccggatcgc cgtcttggga
    20221 tcggacgttc ccagatccac attgaatttc ttccccgtcg catagtcctg gaactggacc
    20281 gggcgtttgc ggcgcaggat cgcctggacg atcgagcccg acgtgagcga ggtgaacagc
    20341 gccatggtga cgagcgcgac gaacaaccgc tcgcggatca gcccggcctg cagggcgagc
    20401 aagcccagga tgatctccat cgcgccgcgg gcgttcatcg cgaaaccgat cgccagggcc
    20461 tcgcgcgcct tgaatcccga catgcgcgcc cccagtgtgc agccgctgat catcccggcg
    20521 ctgccgatca gcagcaccac caggatcagg ccgatgtcga aattggcgac gaagttgacc
    20581 tgcaggccga tgctggcgaa gaagatcggg gcgaagatga acgagatgaa ctgctccagg
    20641 atggtgcggg tgtgcgcgcg cagatggccc gagtcgccca gcgccacgcc gaagatgaac
    20701 gcgccgaaaa tcgcatggat cccgatcagt tcggtcacgg ccgcgcagag gaaaccgccc
    20761 gtcagcgcga agccgaggac gccccccggc cagcgcgtgt gggcctggat ccagggcagg
    20821 atgcggtcga tgagccagcg gccgatcgtc agcatcgtcg cgacgaagag gagcgtcagg
    20881 ccgatggtcc gcccgagatg gctgatgccg ccgccgccgc cgccgagcat ccccaggatg
    20941 atcgcgaaga tgttccagcc gagcagatcg agcaggatcg ccgaggcgac gaccaccatc
    21001 cccggatcgg tgcggaacag attcaaatcg atcaggatct tgacgatgac cggcagcgcc
    21061 gagatcgcca tcgcggtggc gaaaaagagc gcgaagatca gcggatcgga gcccgcctcg
    21121 gcccccgcca accggggaaa gaaccacgcc agcgccagcc ccgcgctaaa cgggaacgcc
    21181 atccccagca ggccgacgtg aaacgccgcc ttcccctgcc ggaagacggt cgacaggtcc
    21241 acctccatgc cggccacgag caggaacagc gagatggaga cggtcaccag cccatggagt
    21301 gcgacgggga aattcccgtc ggtgggaaac agcagccggc tccattcggg cgcaatcgcg
    21361 ccgaagacgg tggggccgag caggatcccg gcgaggatct cgcccaggat cgccggctgg
    21421 cggaaccgcg tggccagctc gcccagcagc cgcgccgtgc ccagcagcac cgccagggca
    21481 aaaaacagga ccgtcatttc gtggtgcgac agcccgttca tggagccacc cgcggccgat
    21541 gaggtgacgg acggtagaac agcagcgcac atgggatctg ctgcaggacg acgctgcgcc
    21601 ggttgtcgaa gaaccggtgc agcagcgaca gcggccgcgg cggggccggg tagaccagca
    21661 ggtcggcgcc gtgcccgcgc gcgtagtcca tcgcgccgac gccctcgccc gcctccaggc
    21721 agtggacgcg cagcttgagc tcgccgaagt cgatcggctc gatgaagttg agcagcgcct
    21781 cgttcatctc gaacagcagc cggcgctgat gctgctccag ttcgcgcggg ctgccgatgt
    21841 cgtactccag cgcgcgcaat tcgagcacac tcagttcgcg cacgacgtgc agggtttgcg
    21901 tcccgacctg gcgggcggtc cggctgatca gcgcgagcat ctggcgcgaa tagtcgtcca
    21961 gccgcgtcgc cacgacgact tcccggaacc cggtgcgggc gggcgcctgg ccctggctga
    22021 gcagcaggac cgagcagggt gcggtcagcg cgacgcgccg cgcgatcgac cccatcatca
    22081 gcgagtcgag caggccctcg cgttcgttgg cgcccatcac gatcagatcg atcagcccct
    22141 gacgcgcctg agcgcggatc acgtgggccg ggcggccgtc gcggaccgcc agcgggtagg
    22201 ccccgggttc caggccgtgg tcgcgcagga tggcctgcag ccgcgcctgc gtcgcctcgg
    22261 ccgccggcgc gacgtgcagc aactccagtt cggcgctgag cgtccgcgcg atcgccgccg
    22321 cttcgctgac gatcacgccg gcatggggcg agtcggccac gcaggccagc acccgcaacg
    22381 gagcaggggc agcttcaccg ggggaactcg gcaggggaca cacggacggg gcctctcgga
    22441 gatggtctgt ggcgagggag gaggccggga accgatacgc ggggtcccgg ctccggccat
    22501 gatcggcggt ggatgctcct ctcatagcac agcgcaacgg acgcgcgcca gccggaaagc
    22561 cccagccagc acgtccctcg tccgtcgtcg ctgatcttcg cccccccgat gccgataacg
    22621 ataccgattg cgatttggaa accgggccag gtccttgctt gcctttctca tagtcgcgcg
    22681 caataacgaa cgtcgcgacg gggcgcggac gggggttggc gcgggcgcgg aggtgtcgcc
    22741 gcttgggggg gaacatgccg agcgattacc agctgattga tggcttcgaa cggctgcgcg
    22801 acgcgtgcca gcgggtcaat ccgggcgtcg gcgcggtgcc ggccgcgccc gaggcggacg
    22861 aaccggcggc gctgcgcgtg gcgctggcct ggctgcgggc gctcgcgccg gcccgcgacg
    22921 cagccccccc ggaacgccgg cggttgcgcc ggctggcgcg gatcctgacc ggggaagccg
    22981 acgacgaggt tcatgaggcc gaaccgcgcg acgagcgcct gctggcgcgc cgcgcggcat
    23041 ggcgggccgg gctggaccgc gcgcagcggc ggcggctgtg gcccctcgcc gacgacgccc
    23101 ccgaccgcag tctggccgag gaggccgccg cgtgggccga ggcgcttccc ggcctgacgg
    23161 gggtgcggcc ctggcggttc ctgcgcggcc tgggccggcc ggtgatcgag ccggagatgc
    23221 cgctgcggcg cttcctgtgg cgcctgggcc tgctcgagcg ggccgacggc gacgacgccc
    23281 acctgcgacg cgcgcacggc gaactggagc gcatcgtgca actgaccggc ctgccggcgg
    23341 aggtgctgcg ctgtctgctg cgctggcaca cggggggcga gcgggagctg accggtgggc
    23401 gcgtgtgcgg cgcccggccg cgctgcgcgg gctgtccgtt cggggagctg tgccccttcg
    23461 cgcggtacaa ggcggcgccc gcgccggatg cggacggcgc ggccgaggcg ctggcggagg
    23521 ccggccgccg gctgcgcgaa ggacgcgccg ccgagctcgg cgaggcggac ctgctggcgc
    23581 tgacgctggc ggccggcggc atgccgcggc gggcggcgac gcgcgccgct gcggaattgc
    23641 tgcgtcaata tgggaacctg cggacattgt cgcaggccac gccgccgcag ttggccgagt
    23701 cgccggggct gacgccggcg cgctgcgccc tgctgctggc cgcgctggag ctgggacggc
    23761 gcctgggcgc gctcgcgctc cagccggggt cgccgatcac gtgcagcgag gaggtgtggc
    23821 aggcctaccg cctgcgctac cgcgacctgg cgcaggagca tttcgtcgtg ctcctgctgg
    23881 acgcgaagaa ccgcgtgatc cacgagcatg tggtgagcaa ggggacgctg acggggagcc
    23941 atgcgcaccc gcgcgaagtc ttccagcagg cggtgcgccg gtcggccgcg gcgatgattc
    24001 tgctgcacaa ccatcccagc ggcgacccga cgcccagccc ggaggatcgc gccgtcacgg
    24061 cgcggctgcg ccaggcgggc gagatcctcg gcatccgggt gctggatcat atcatcctgg
    24121 gcgccgagca gtactacagc ttccgggacc gggaagacta gacgatgccc atggcgacac
    24181 cgatcacgat cccgatgccg atcccgaaag cctggctatt ctgcgttgct tacccgtcgc
    24241 cgcggacttc gtagacgctc tggacgccgt accagtcggg gttcaggtcg ttgaggttgg
    24301 tcgtctcgac gaggcggaga cccagatcga gcgcggggtg gggcttcatc tcgagatacg
    24361 gctttttctt cggatgcggg atgcgcggca accccagcgg cttgcgcagc cggttaagcc
    24421 agcgcgtcca cttcagcccc tgacgttgcg ttctgcggcg tttcatcgtc gggaagcgct
    24481 tgatgtagtc ccagaacggg atttcgcggt ccccctcgcg gatgatgatg acgtaatcat
    24541 agggcttggg gaatcgcttg cggcggaccc agacgtcgat ccgggcgatg ccgtcgggcc
    24601 gcagcacccg gctgatttcc tccagcaggc ggatcttgtc gggcaccatg aacacgctgg
    24661 cctggctata gaccagatca aaatagccgg tctcgtggcg cagaccctcg ccggcgtcat
    24721 agaacgcgac cgcggggggc gtcacggcgc gcgattcttc gtcgctgaac aggcccatgc
    24781 ggaccgccgt gcggcggatc cgccgccagt tgccgtggtg ttcgtaacgg ttgatgccgt
    24841 gcagttcgac ctgatcgccg taaagcttgc gcaaatccat cagcgcggcg ccgaagccgc
    24901 atcccacctc gaggatccgg acgggccgcc cctggccgag ccgggcgtcg atcagcggcc
    24961 gcagatcgcc gatgcggcgg cgcacctcga tcaggccgcg ggtgcgcgag ggtttcttgc
    25021 gccgcaccag tcgccgggtt tcagcaggcg cgaaccagcg cgagatgagc gtcaggaagg
    25081 gcaaggtcgg tcgatcatca ccaagacggt tcggctccaa cggccgggaa tgcaccgcgg
    25141 ggaacctccg gcgccgcagg acgcggcgcg aaggggagtc tcaccctagc atagagcaac
    25201 gcaaggcaca agccggccgc ggcgcgctcg gctcagtggt tgttcttgcg ccggcttgcg
    25261 attaggcttg ggcggtaggc gccatgtcca ccgcgacccc cccgattccc gcgtggttgt
    25321 ttcgagccct cgagcggctg ggggagccgt cgcgcggcgg gccgcggcgg gccgggctgg
    25381 gggggcaggt caccgcggcg gcgatgctgc tgctgttcgc gctgccgggc tcgctcgtcg
    25441 gcatcgcgat gatccggttc tggaatcgcg acgcggcgcc ccggtgcttc catgcgctct
    25501 acgacagcgg cctgatgctg ccgctggggc tggcggcggt gcacctgccg ctggcgtggc
    25561 tgctgctgca cggccggctg cgcgccacgc cggcggtgct gggcgaagtg gagcggctcc
    25621 aaccgctggc gccggggcgg cgctggctgg agctgagcgc gccgcggctg gccgtggccg
    25681 gcgcgctcgc cgccgggctg gtcttcgtgc tcgccctgag cgaggtgcat gcctcgatcc
    25741 tgctggcggc gcccggccag gagacccttg cgatccgctc gttgacgctg ctgcactacg
    25801 cccccgaccg tctggtggcg gcgttctgcc tggtgcagct tgcgacgacg gcggccgggc
    25861 tcgccctgct ggcggcggcg agcgttgccg gcctgaaact gtggaggcga ctggccccgc
    25921 atcatgccgt tgattgagtg ccgcaacctg cagcgccggt tcgggcgccc gccccacgag
    25981 gttgaggcgc tggcgggcgt gtcgctggcg gtcgacgccg gccagtgggt ctgggtggtg
    26041 gggccgtcgg ccagcggcaa gacgacgctg ctgcgcgcgc gggcgggacg gattgaacca
    26101 tgacgctgct ggccgatttc gccgcgtccg accggctgct gcaccgcatt tccgggcccg
    26161 agcaggcgcc ggtgctggtt tacgtgccgg gaatccatgg cgactggacg ccgatggtca
    26221 actgccggg
//
LOCUS       DAOPCO010000029        26188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2033_length_26188_cov_5.728236, whole genome shotgun sequence.
ACCESSION   DAOPCO010000029 DAOPCO010000000
VERSION     DAOPCO010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 26188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26188
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2033_length_26188_cov_5.728236"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1161
                     /locus_tag="PLS90_03965"
     CDS             100..1161
                     /locus_tag="PLS90_03965"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPK01592.1"
                     /translation="MIRPLSRLTGDPRWAELEPERILYLDTETTQLSTAAGAYAFLVG
                     LGRFAGGTFVLDQYFMEDHGCEPALVEHLDAALAEARALVSYNGRCFDLPLLETRWRM
                     QRRAPRFPELHLDLLHTARRLWRLRLPGCGLGDVERHILGIRRLSDIDGALIPRIYLD
                     FVRGVRPERIVPVFDHHAQDVISLGALASAVTHAVRRPDDPRFAHAGDQWGLARLLER
                     LGEARRAIDCLEAAVLAARDEAFGYRLAMHLARAYKRCGRVADAVAIWEARAAQCRPG
                     RLEALVCLAMHAEHALKDHAAALRYAERALAVARQGAELAWIGTAATAPRAEKRYEQE
                     LDGLRRRIERIERKIEKTR"
     gene            complement(1168..1716)
                     /locus_tag="PLS90_03970"
     CDS             complement(1168..1716)
                     /locus_tag="PLS90_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK01593.1"
                     /translation="MSSLLRKLQKKEFRDAYAADHLAVGIPFQIRALRKQRGWNQAEL
                     ARRWKKQQSWVSEIEREGKASYTIATLSELAAVFDVALVVKFVPYSRFLKEYEDVSES
                     ALEAASFEDELPRLKAEVEGNGLAIGDGAGTEAERQAMVGLSFSQVKALRDALNRVLK
                     ENDPLSASPHINDLEPASGSKQ"
     gene            complement(1709..2095)
                     /locus_tag="PLS90_03975"
     CDS             complement(1709..2095)
                     /locus_tag="PLS90_03975"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPK01594.1"
                     /translation="MEWKLRGFLTERGVNVVDEWYENLPPKAQARFVVIWQYLSVRPI
                     SEWIRPYSDTLESGLREIRMEVLNIQYRPIGCFGPHDREVFTILICAQERDTKLVPRN
                     ALSLAAARRAIILNDRRRASDSRILE"
     gene            complement(2225..3208)
                     /locus_tag="PLS90_03980"
     CDS             complement(2225..3208)
                     /locus_tag="PLS90_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TagF domain-containing protein"
                     /protein_id="HPK01595.1"
                     /translation="MKFGALQNLKDRFFSRGGGAIESFTLSAYGKLPIYKDYILWECL
                     NDGAEDFKQWLDAAFGKTWEDFDGKSARLRGPCRALILLPDGKYAVAASMWPSADEGN
                     MRKFPFALFTTFSRGDVVGRGLRGALDALAPVWLSLEREYEKIRQLGSIDELYAYLQR
                     TDPSVQPPEPAPEQNLSLEQWHEALCPGRGRAFRRWIDHELNQVISAYRGFPEQGESL
                     AVRLPLAGRLDIPTQAAIWESVFKQNLKKIPVFPSVVIEKGEDDEPGAITLVWREWLV
                     DDARLFADKIRDYEFVEMLIPDKLAGDGADGELEDDLEIEAWIASFGKVKA"
     gene            complement(3275..7390)
                     /locus_tag="PLS90_03985"
     CDS             complement(3275..7390)
                     /locus_tag="PLS90_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HPK01596.1"
                     /translation="MIQNLAHMPFLYRFMAGGTFAIGAISIVGRIIGWKIASILMVGL
                     ILASIVIFLHNKGLKLRRRKKASAFDKGLQADANKPGKGKAEQRKAMGELSQRWNQAV
                     GQLKASGLSLYDLPWYMLIGEPQSGKSTTLKFSGLEFPVGTDALSGAGGTRNCDWWFT
                     NEAIILDTAGRFTFQEKNAPDSEEWTGFLKLLKKYRPACPINGVVLTIPCTSLLGDSP
                     EEREAKAQNIREKLTQIQRELEVQFPVFILITKSDMILGFTEFFSRLGATEQRQLLGW
                     SMHGPFIQAYDPDKFDSVFQQIYDRVHKWRNKILGEDLSLTEIDKLYVFPEEFKSIRE
                     PLNDYLRTIFVKTHYLPPLFFRGFYFTSGMQTGRPIAKACAAMLRSAGEGADEIIEHL
                     QKIFERSRAFFIRDFYVEKLFPERNMVIHSEKRLKTGRKLAIAAIWGSVAVILLTLGL
                     CGWSLWNMRGGVGDVEKHARTIKARFISQKDSPIDAIGTLSQYSESAEETEARFPGLH
                     RDLRDIYWLYFMRNIQLANADRLYQSLRATVPGVTIKNPEDPATRLAVAKYTGYTRNW
                     SINDDWINHATSSSKPYRHSADLGMTALFDEQGNLLQDQELTEKYKQHLERVRAIVGA
                     GDEEIDFSDMPIAIPLVSGEANPRTNEVISKEIQYVKEVAADVPTQETVNNVVDAAKA
                     FLQGYPDSIAGLLGDVGCDLNSVPFAAMADSINRLKTGFGEIDAILAANRDDQGEMPE
                     ASFDYSQAFAQLAWASAAIADTERTAQLNALINEAKQQLSAEMEQQLAANRDQLATVS
                     NFITSGFTLSEGAALLRQGLPLIEALIAAKDEMLARAADASPDSVEAANVVHAEWLAN
                     AARAAAMGWAAAAKTGTADPWRLPEIGARVEQSILAIDEAVLTQAMVAYFRRAVPGTL
                     LSSGQISGASLTSSPPSGAQPSPVAKANDEIAKLVAAIDDFKGKLTCDANKVRLNSLR
                     ESAQGVADANISNFNRYWVNAYNNWDPEAWIRSIPTWAAFKSDPLMQPGNLAGRVRED
                     IASLIANVGGFSDQVGKVSSELNQIYIQLSGASAQLEPKINTFTDTVGGMSVDEMQAL
                     MQLNQQATSDRLGQIASNSKFDYYPQKVRELLGGAGNLDEKWDTFIAKWRGKLAGKFP
                     FKIASGSVSRSDGQVVIPQAEATDFQAFFHGPDSLPAFIKEYQEVWDEWIARPQNASK
                     KRFADQALALGGFFFDGDDYRLVSINGTYQTFEGALEADLAQISESIILAVPTLAATP
                     EEFRWRTLMKGQSGDFTWRPGNYGNAFTFRSELDRSEDANKAEMRVPGDFSVLAYIVT
                     QSPGAEAERRRWPVQFSVKPEGIRRDQLYNINFLFVLDRSMPALPDWDDLK"
     gene            complement(7489..8445)
                     /locus_tag="PLS90_03990"
     CDS             complement(7489..8445)
                     /locus_tag="PLS90_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type IV/VI secretion system protein"
                     /protein_id="HPK01597.1"
                     /translation="MNLTEVASDLFLFLVTFRRQVRKGLIPDIYQVRSQLLDIFESIE
                     MRVRDDARLAKLYERAKYPLIVTADEILINANWAYSSQWEDEILEYEFFKTRIAGERF
                     FEMINELEDDQEELAEIFYHCMCLGFVGRFKDDAEELRRLKRRLYRMLPGRVSDEEKR
                     ITPDAYYVAEGVKDIYKPLVNLTRILIICGTLLLVLWGGFFYYKRFVVPGAIREIAQA
                     MREDARPAGADVEDEADEAETAIETEATATPTPDPARDPFATLSAPAATPAAPVVAPA
                     LPAAAETTAPAEATPTPSPTPTPTPTPTPTPTPTPTPEIPAI"
     gene            complement(8595..9962)
                     /gene="tssK"
                     /locus_tag="PLS90_03995"
     CDS             complement(8595..9962)
                     /gene="tssK"
                     /locus_tag="PLS90_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HPK01598.1"
                     /translation="MSEFMEINWCEGMFLRPHHLQQASRSQRAALHREIGIARPFAYG
                     IQQINISVAELEGFTFNIRACNLRLKDGTRLIAPHNGEFQPRSFKKELDAAGGVLDVY
                     IGLPLLMEREPNTLLLSEESSIYHRRYKAVVSEVVDENTGSNGQAIEFRKYSPRIFFR
                     GEDTTGYETLQIARIERSGFEENKPILSRNFIPPILVMEAWPPLFDYCKDIYQQLVAK
                     NRSLISQIAGRKIAFGSEGVGGPEAMLKLNITNRYVAFLRQWTSMPNLHPFDVYVELC
                     RLAGDLAIFDEGRAAPELPLYNHDDLGACYLDLLRLLERLINNILPTTFIKRRFEPVE
                     NRREVALEDEWLLPEVEFYLCIESDQDDDAIDRQVSYLKMGSVEDLPTLINRRLPGLM
                     LRRVRRTPIGLPDRPNYHFYRMSREGRFWDSLLQTKVLASYGLADETLELDLYILLKP
                     EYEGD"
     gene            complement(10131..10658)
                     /locus_tag="PLS90_04000"
     CDS             complement(10131..10658)
                     /locus_tag="PLS90_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01599.1"
                     /translation="MRKALWLMLAACGLAMGCSRLPIPTEQITFYRSDALNEGRPCAV
                     DVVYPKDEDQLYQITREIGPAGWFRSDLYRTAYKDKEWLDEKSTTLMLSNKDEQDRFM
                     VIIAEFTNPDPNPPRGQEPILVFREDMKPRPRKHEYIWVHDGSLQRLDGRPKDLRGRE
                     DHEDDRRARDRDRRD"
     gene            complement(10841..11596)
                     /locus_tag="PLS90_04005"
     CDS             complement(10841..11596)
                     /locus_tag="PLS90_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01600.1"
                     /translation="MTRPAATANPIIEVHGVSKRYRLAEVEEVWALRGVDLTIYEGEF
                     LSIMGPSGSGKSTLFNQIGALDIPTEGRVLFLGQSIFDMTESQQAWFRCHKIGYIFQT
                     FNLVPVMSALQNVMLPMIFAGTAQEEAEGRAAAILERVKLGHRLHHKPFELSGGQQQR
                     VAVARALANQPTVILADEPTGNLDSHTGREIVELLRELNTGEGVTVVCSTHDPKMLHA
                     SDRVCWIRDGRLERIVSGEQFRLETMAAGDAQH"
     gene            complement(11626..12402)
                     /locus_tag="PLS90_04010"
     CDS             complement(11626..12402)
                     /locus_tag="PLS90_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015337304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01601.1"
                     /translation="MTLNREKILKLFEPPAQAPPSRVIQAVGVRKVYRTGDQETHALR
                     GATLEIPRRCYLSIMGPSGSGKSTLFNMIGALQKPTAGKVYIDQVDIAHLDTDELAWL
                     RCRKIGYIFQTFNLIQVMTCLENVTLPMAYAGLEGEAARDKGIHLLQLVGLGHRILHK
                     PAELSGGQQQRVAIARALANSPEIVLADEPTANLDQKTGEEMIELLARLRDELGVTIV
                     SATHDLKMLRRSDRILWIEDGRITRSARPDEIDFDHAEFH"
     gene            complement(12587..13579)
                     /locus_tag="PLS90_04015"
     CDS             complement(12587..13579)
                     /locus_tag="PLS90_04015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01602.1"
                     /translation="MLNREPTTIPSQIDLPPRVAVQIVSQGIRIRFGRSLVTITGVLL
                     GIAFLSAAWTSQLIRHGVREEDRLRAEAGRMLSFLEAETGPVRDRRIVIYSAGALAAE
                     ETKLLEQLQRRRAEVIAGDAATSEALGNAAAVILAGAGPFDAVPWTALASRSVVAPTR
                     SAAARPPQLTARWVPLAREIRPEEQARLEQEQLRERYRLVWILSIALLVTVMGISNAM
                     LMSVTERFREIGTMKCLGARSSFIRRMFLIESGFMGFCGGLAGALLGCLFAIALQAVS
                     YGPALVLASINYLALAGVIGGTLVCGILLSVVAALYPAAVAARMTPAAALRSNV"
     gene            complement(13579..15657)
                     /locus_tag="PLS90_04020"
     CDS             complement(13579..15657)
                     /locus_tag="PLS90_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HPK01603.1"
                     /translation="MARDTHDREVELYRSLLETPAHFREGFSATTVVGIFFCGLVMMP
                     GAIYLGLMTGGNLGPAAAWVTVILFMEIARRALKPLASQQLVVLLHAANVMMAAHILF
                     PGGPMGQLVYRAYLVLSDAARDAGMALSFPEWFVPPPGSDALAERTLWHRDWLVPILI
                     IAFMMVIGLIKRYTLGYFFFRLTSDIERLPFPLAPIAAQGAMALAEAEGEAPTADDPD
                     PRGKQTRGKTSRWRIFSLGAIFGLGFGLLQIGIPGVSSLFLAKPFFLIPQPFVDTTVL
                     TQGILPATPTGLTFDLGIVFLGFVLPFWVVIGSFLAIATTILINPILYHAGVLTRWQP
                     GMDTVNTTFANHIDFWMSLTIGTALGIAVVCFYATLRDVVVRVREARRQRAAGAAPRN
                     LFQDHPAGRGDYPLWIALVLYIGAASAVVALCWFLLPKTFSIFFFLIFFTFAYTPLIS
                     YVNARLLGISGQNVEIPFVKETAFILSGARGIDIWLAPVPVENYGYMAQAFRVNELTG
                     VRFPSLLKTELVAIPILLVLSLVYWSFIWHSDPIPSDLFPAAQVNWELQAKNQVLLYS
                     STFVGPGETGGSNVADSEFMQAIHPWVIGSGFVGCIALYAGLSLLGLPIMLIYGVIRG
                     LGQLPHFMILEVVGALLGRFYFQKRFGQVNFLRLAPALLAGYYTGVGLISMMTMALML
                     IKSAISSAPF"
     gene            complement(15657..20918)
                     /locus_tag="PLS90_04025"
     CDS             complement(15657..20918)
                     /locus_tag="PLS90_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01604.1"
                     /translation="MRRPPRHFPIVSHRRRGAAGRRHVSALGALFFLVAAAAVWLLGL
                     RVFAPRPYLEPPRPDYSYDPRPMQQAVDWRAVQARQEAIVGLGSRFMGQPGVYAAEAL
                     IRDAFLAAGLELHEQENWTVVPRTIRREIALAEDEGTTLPDVEIFPFMPNHLQPVATP
                     DEGVTGELLVLTPEVLRTRERFDDCIGLVDASPGRYSPDYEFAWGRYAQLGLRALIVS
                     HPEGLDQVPLERVVPWQKGTPSKGGMVSSMPVNFVRVAATPAIFDYAGRRVTLRVNVR
                     FEETRNTSLVGILRAPGTSRERPNTDALVILSNYDAASILPDRAPGVIQAISPAAQLA
                     LLEGFTAYRDSLVRDVIFIACGAQMMARAGDENLLRLLDENIYKATRNPVREILGLGA
                     AEAGADAQAGARAARLQPWRERHEENEAALRRTERILALFENDGFLSEPGPTLAALAQ
                     LDEDDAAFLRDQVDYVLDTLAFEQYEAQLQAKLVFLTTGENLHGPEFAAFQEARRRYD
                     RAVAVGGRRLENLFAAESGLGDFLNQHDLRRRWRARFEELRAYHQRRRRQLTLDLDVL
                     AALNPYQQFIIFDNKLVPAFHADRREEVLTFSNGDWSVSAQMRAMASLFVSARQRLAA
                     FTGNADLRRELEIPRLAKYHNQDVDRNTQPVSDRSSVMWMRFGYLNFTLLNFDRQQSY
                     EHWADPTDRPFMHDVASLRHSLAVVGENLLSIAHGNGKLAAIQVGWLKRTFGGRVLAD
                     GIGQSLVPDYPLAGAVLANRTYLNWETYAFPGYYDHILIMTDPYGRYELVNSASDFWV
                     SQNVWANGYSPLAAWHDRNGFIRWMKDEGEDGQRLYKSVKLNWFDAKVDNITLVCFRA
                     APVAFFDLTNPQTLRDYSGLRLFSRDGLAEFRKRCLYETNAMRMAWLDPEARFYAALE
                     SGSPENELVKEIRGFLLGVPRAGEPSDFVPDPDREIDGPGYLAAATPVMQGLPFEMGE
                     SMTFLNGRRLEVQNRHRMADPRTNDYHAKARGLLAESAAPELPWREKILLAREAVTYA
                     MLNHPVLRRSLFEAVLGIIWYLALLVPFTFFFEKMVFGYSDVRKQIAAQIIIFLVAFG
                     LLRVLHPAFGMVRSSVMILLGFVVILISTGMTILFSGKFKENLEELKKRRGQVEAAQI
                     NRLGVIGSAFMLGLNNMHRRKVRTGLTCGTLTLITFAMIAFTSVHSDVVDEATALGKA
                     GYQGILIKNEHFKRISDAELFAITERYGDRYQVNPRRMYLGQQNWQDTQGYNPELVVL
                     YETDGRRRAAQADSLMQMSAGDPLRDQIRLLTDRGWFTPADEASAELPAAILPDRMAE
                     RLGLTAAEVNTGDVQVQINGRAFRVRGIFESRSLAEMRDLDGRDLLPYDIEAMAHIQL
                     QEGEVVATDADPRIDPARIILAPQRELNLQVPNGIPAIVSVAVAMPQAGYAEARAEID
                     DYLEQTGKTAFYGLEGVAWRGRRAREASFEGLIELLIPLILAALTVLNTMKGSVYERR
                     DEIFVYNAVGIAPRYVFFMFIAEAFVYSVVGTVLGYLLSQGVGRILTELDLTGGLNMT
                     YTSINTIYASLAVAAAVFISTWFPARAAMEIARPAEEAGWSLPEPDGDDLRFDLPFNF
                     DHRDRIAVLAFCERWLADHGEGGGGRFHASVPELGVEQPSAAGDDDYLPAIRATIWPK
                     PFDLGVSQRLEIVMTPDPETGEFKAGVRLERLTGTREAWLRLNKAFVADLRRHFLHWR
                     AVDESDRRNLFDEARDHILTNLGLEHA"
     gene            complement(20918..23503)
                     /locus_tag="PLS90_04030"
     CDS             complement(20918..23503)
                     /locus_tag="PLS90_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01605.1"
                     /translation="MNPAPRQTGGSAARAMLAALFGLLLVGLMVQFFQLIEAGGDMVG
                     AHALPIPAVVVVLGLVVLTGAAWRLLRVRLLGRGELIALFFVLMMAVPLMSSGFWSYM
                     IGAIGAIPKTADFDAYDAYPKKLWPHGRNLLAGALTPARRADLVTSGTVLWETIELEH
                     APSEERPVLVNQSPEDVSWVRMRVALAAPAGAGDPFHQLIPEEPYLVTLLLRPHELGP
                     EAQYACRLYYDDRAEFGAEAFAARMAGEVSYLHPLGFLRRGMYGLTVPRDVQRHVDLE
                     LALEGRGRLELADVELISVGALHSIYYGREVINASRFAELGESPRTELLVRPDNLWSV
                     AGLRFLLAGYIPWREWLAPIVAWSGIALLVLLGTFCLAVLMRSQWIESERYPLPLTRI
                     PTAIVGEPDAPPSDHALAPIWRNRLMWLGFAVTLFWCLMKGWHQFNTSVPDMRIAVEL
                     TPYFTDPGWGNMWRGQEVRNVTFAVFAFYVGIAIFIESSVLLSLIVGFFLFRCQYWFG
                     EATGLALTAEYPFVFQQQVGAFLTYGVLILFFTRKHLARVVSAAVRGRKEAWRGEALS
                     YPAALAGIAVSVGGIALWAWWVGLAVPAMLVFFAALLLIGLVSAKIRAECGTPMGIFM
                     PVALSKLVAIAGGMEFFGPAGVLFVSFANWIIFRHLFFLIPGMQVELLELGRRYRLPP
                     RAITGAALLGVVGGIFLGGWIFLSLGYAIGGDNFTHRYPYMDKGHLVNEYNLELAKVN
                     PAMPAPDTGIDPPTWGYVFAAGVTFVLTVLRQIFSGFWFHPVGFVVSSMPMLEYVWGS
                     LLVAWAIRVVTLKLGGAVTVRTRLMPFFIGVFLAAVAAYAIFGAINTYLYFFHPTLVR
                     ARLLF"
     gene            complement(23560..24531)
                     /locus_tag="PLS90_04035"
     CDS             complement(23560..24531)
                     /locus_tag="PLS90_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPK01606.1"
                     /translation="MSVATEAAPRQGLIGAESLLKRYPGNPIIPRNALPDSDSILNSA
                     VVRFGDAYRGMFRVDNQNRDMLIHPGRSADGLHWEIDPRPVQWQCRDSELEQIVYAYD
                     PRITPLEGRYYITWCHGYHGPCIAMGVTDDFEKFELLEVVLPPFNRNAVLFPRKINGR
                     YVMLHRPSDNGHTPFGDIFLSQSPDLIHWGSHRFVMGTAGGWQSTKIGAGPVPIETDE
                     GWVMIYHGVLTSCNGFVYSVGCAILDLDQPWKVLHRTRRYILAPTEPYEMVGSVPNVT
                     FPVACIHEEKTDELRVYYGAADTSVCLATTTLRELIDFTKEHDMLKS"
     gene            complement(24674..25870)
                     /locus_tag="PLS90_04040"
     CDS             complement(24674..25870)
                     /locus_tag="PLS90_04040"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK01607.1"
                     /translation="MSKEHPQLFGRPIFPPDQGLTLTSQVREILLEEIHGGHWEVGER
                     LPSVATLARQSGLSRWPIQEAFESLRKEGYLRQSSRSGTYLESLAPQGRKPLGSIGIV
                     LMLEEEQGNWKTPPFTQYRLGRVIEMAEQRNFAVEVKYLRPSDDWSRVDQVGGVFSDK
                     VIGVIALNPFHHPAAFELPPDRLPFVHIGGASQECHPTVAGDTMLGFYKLTRRIIEVG
                     HRNIICFCNPTDPEWETQSRLDGHARAMNEAGLRVNEEACRRSMTIAEGDLAGLRTFL
                     EEFRDATAIICMWGGVDFHIVQVATMMGIRVPEDLSVTGHGAGPMGTKSGATMTCLEY
                     DMNAMLNACFDLLQVQRTRRRAEFSQILSNPLIHEGDSLAPPPPRRPALAGAASGLAG
                     RNRYLR"
BASE COUNT         4509 a         8135 c         9093 g         4451 t
ORIGIN      
        1 gccatcgtca acacggcgtc ggtggcggac tcgtccggcc acacgggctg ctgttgggcc
       61 ggacgctcgc gccagctcga tggattccac tcagcggtca tgatccgacc attgtcgcgg
      121 ctgacgggcg acccgcgctg ggcggagctg gaaccggagc ggatcctcta tctggacacc
      181 gagacgacgc agctttcaac ggcggccggg gcctacgcgt ttctggtggg gctgggtcgg
      241 tttgctggcg ggacgttcgt cctggaccag tatttcatgg aagaccacgg ctgcgagccg
      301 gcgctggtgg agcatctgga tgcggcgctg gcggaggccc gggcgctggt aagttacaac
      361 gggcggtgct tcgacctgcc gctgctcgag acgcgctggc ggatgcagcg gcgcgcgccc
      421 cgctttcccg agttgcacct ggacctgctg cacacggcgc ggcgactgtg gcggctgcgc
      481 ctgcccggct gcggcctggg cgacgtcgag cgccacatcc tgggcatccg gcgcttgagc
      541 gacatcgacg gcgcgctgat cccgcggatc tacctggact tcgtgcgcgg cgtgcggccc
      601 gaacggatcg tgccggtctt cgatcaccat gcgcaggacg tcatctcgct gggggcgctg
      661 gcctcggccg tgacgcatgc ggtgcggcgg cccgacgatc cgcgcttcgc gcatgccggg
      721 gaccagtggg ggctggcgcg cctgctggaa cggctgggcg aggcgcggcg ggcgatcgac
      781 tgcctggagg cggcagtgct ggcggcgcgc gatgaggcgt tcggctaccg cctggcgatg
      841 cacctggcgc gcgcctataa gcgttgcggg cgcgtcgccg acgcggtggc gatctgggag
      901 gcgcgcgccg cccagtgccg gccggggcgg ctggaggcgc tcgtctgcct ggcgatgcac
      961 gccgagcatg cgctgaagga ccacgcggcg gcgctgcgct atgccgagcg ggcgctggcg
     1021 gtggcgcgcc agggcgccga gctggcgtgg atcggcacgg cggcgacggc cccgcgtgcc
     1081 gagaaacgat acgaacagga actggacgga ttaagacgcc ggatcgaacg gatcgagcgg
     1141 aagatcgaga agacccgata gaagggacta ttgcttcgaa ccagaggcag gctccagatc
     1201 attgatgtgg ggactggcgg aaaggggatc attttccttc aagacccggt tcagcgcgtc
     1261 gcgcagggct ttgacctgcg aaaacgacaa tcccaccatg gcctgtcttt cggcttcggt
     1321 tccagctcca tccccgatag cgaggccatt gccctcaact tcagccttga gacggggcaa
     1381 ctcatcctcg aacgatgccg cctccagagc gctctccgat acgtcttcat attccttcaa
     1441 aaaccggctg taagggacga atttgaccac gagcgctacg tcgaagaccg ccgccagttc
     1501 cgaaagcgta gcgatcgtgt aggaagcctt tccctcccgt tcgatttccg agacccagct
     1561 ctgttgcttc ttccatcttc tggcgagctc cgcctggttc catcctcgct gcttgcgtag
     1621 cgcccgtatc tgaaagggaa tgcccaccgc cagatgatcc gctgcgtagg cgtcacgaaa
     1681 ctccttcttc tgaagttttc gcagcagact actcaagaat ccgtgagtca ctggctcgcc
     1741 tcctgtcgtt caggatgatc gctcgccgcg ccgcagcgag agacaaagcg ttcctgggaa
     1801 ccaacttcgt atcccgctcc tgggcgcaaa tcagaatcgt gaagacctcc cggtcatggg
     1861 gaccgaagca gccgatcggg cggtactgaa tattaagaac ctccatcctg atctctctca
     1921 ggccggattc caatgtgtcg gagtagggtc ttatccattc tgaaatcggc cggaccgaaa
     1981 gatactgcca gatcacgaca aatcgggcct gtgccttggg cggcaggttc tcataccatt
     2041 cgtccacaac attaacaccg cgttcggtga ggaacccccg cagcttccat tccataatat
     2101 cagaaatatc cgatcagcca aatgaaatag atcagtaaat tcttgtatac ggattagagg
     2161 gcatgaaagt atgaattatc ggataactcg agcgagacgc ctgaatccgc tcttccggtt
     2221 gcgctcaggc tttgactttg ccgaagctcg cgatccaggc ttcgatctcc agatcgtcct
     2281 ccagttcgcc gtcggccccg tcgcccgcca gtttgtcggg gatgagcatt tccacaaact
     2341 cgtaatcgcg gatcttgtcg gcgaacaggc gggcgtcgtc gacgagccat tcgcgccaga
     2401 ccagcgtgat cgcccccggc tcgtcgtcct cccccttttc aatcacgacg ctggggaaca
     2461 ccgggatttt cttgaggttc tgcttgaaga ccgattccca gatggcggcc tgggtcggga
     2521 tatcgagacg gccggccagc ggcaggcgca cggcgagcga ctcgccctgc tcggggaagc
     2581 cgcggtaggc gctgatcacc tggttgagtt catgatcgat ccaccgacgg aaggcgcgcc
     2641 cgcggcccgg gcagagcgcc tcgtgccact gctcgagcga caggttctgc tcgggcgccg
     2701 gttcgggggg ctgaacggag gggtcagtcc gctgcagata cgcatagagt tcgtcgatcg
     2761 agcccagttg ccggatcttt tcgtattcgc gttcgaggga cagccagacg ggcgcaagcg
     2821 cgtcgagcgc gccgcgcagg ccgcggccga cgacatcccc gcgcgagaac gtggtgaaca
     2881 gcgcgaacgg gaacttgcgc atgttgccct cgtcggccga gggccacatc gaggcggcga
     2941 cggcgtattt gccgtcgggc agcaggatca gggcgcggca gggcccgcgc agccgcgcgc
     3001 tcttgccgtc aaagtcctcc caggtcttgc cgaaggcggc gtcgagccat tgtttgaaat
     3061 cctcggcgcc gtcgttgagg cactcccaga ggatatagtc cttgtagatc gggagtttgc
     3121 cgtaagccga gagcgtaaag gactcgatgg cgccccctcc gcgagagaag aagcgatctt
     3181 tcaggttttg tagagcgccg aatttcatcg ggcacctgtc cctttcatca ctccacgcga
     3241 gaatcggtga gccggccgga ggccggagac tgcgtcattt caggtcatcc cagtccggca
     3301 gggccggcat gctgcgatcg aggacaaaca ggaagttgat gttgtagagc tggtcgcggc
     3361 ggatcccctc gggtttgacc gagaactgaa ccggccagcg ccggcgttcg gcctcggcgc
     3421 ccggcgattg ggtgacgata taggccagca cggagaaatc gccgggaacg cgcatttcgg
     3481 ccttgttggc gtcctcgctg cgatccagtt cggagcggaa cgtgaaggcg ttgccgtagt
     3541 tgcccggccg ccaggtgaag tcgcccgact ggcccttcat cagcgtgcgc cagcggaact
     3601 cctcgggcgt ggcggcgagc gtcgggacgg ccaggatgat cgactcgctg atctgagcca
     3661 ggtcggcctc gagcgcccct tcgaaagtct ggtacgttcc gttgatgctg accagacggt
     3721 agtcgtcgcc gtcgaagaaa aacccgccca gggcgagcgc ctgatcggcg aagcgcttct
     3781 tcgaagcgtt ctgcggccgg gcgatccatt catcccagac ttcctggtat tccttgatga
     3841 aggccggcag gctgtcgggc ccatggaaga atgcctggaa atccgtggcc tcggcctgcg
     3901 gaatgacaac ctggccgtcg ctgcggctga ccgagccgct ggcgatcttg aacggaaact
     3961 tcccggccag cttgccccgc catttggcga tgaaggtatc ccacttctcg tcgaggttgc
     4021 cggcgccgcc gagcagctcg cgcaccttct gggggtagta atcgaatttg gagttgctgg
     4081 cgatctgacc gagccggtcg ctggtcgcct gctggttgag ctgcatcagc gcctgcatct
     4141 cgtcgacgct catgccgccg acggtgtcgg tgaaagtgtt gatcttcggc tcaagctggg
     4201 cgctggcccc cgagagctgg atgtagatct gattgagctc gctgctgacc ttgccgacct
     4261 ggtcgctgaa cccgccgacg ttggcgatga gcgaggcgat gtcctcgcgg acgcggcccg
     4321 ccagattgcc cggctgcatc agcgggtcgg acttgaacgc ggcccaggtc gggatggagc
     4381 gaatccacgc ctcgggatcc cagttgttgt aggcgttgac ccagtagcgg ttgaagttgc
     4441 tgatgttggc gtcggcgacc ccctgcgcgg actcgcgcag gctgttgagc cggaccttgt
     4501 tggcgtcgca ggtcagcttg cccttgaaat cgtcgatggc ggccacgagc ttggcgatct
     4561 cgtcgttggc cttggcgacg ggcgacggct gcgccccgct cggcgggctg ctggtaagcg
     4621 acgcgccgct gatctggccg ctggagagga gcgtgccggg cacggcgcgg cggaaatagg
     4681 cgaccatcgc ctgggtgagc acggcttcgt cgatggcgag aatcgattgc tcgacacgcg
     4741 cgccgatctc gggcagacgc cacgggtcgg ccgtgccggt cttggcggcg gcggcccagc
     4801 ccatggccgc ggcccgcgcc gcgttggcga gccactcggc gtggacgacg ttggccgctt
     4861 ccaccgaatc gggcgaggcg tcggcggcgc gggcgagcat ctcgtccttt gcggcgatca
     4921 gcgcctcgat cagcggcagc ccctggcgca gcagcgcggc gccttcgctg agcgtgaatc
     4981 cggaggtgat gaaattgctg accgtggcaa gctggtcgcg attggcggcg agctgctgct
     5041 ccatctcggc gctgagctgc tgcttggctt cgttgatgag ggcgttgagc tgcgcggtgc
     5101 gttcagtgtc ggcgatggcc gcgctggccc aggcgagctg cgcgaaggcc tggctgtagt
     5161 cgaagctggc ctcgggcatt tcgccctgat cgtcgcgatt ggcggcgagg atcgcgtcga
     5221 tctcgccgaa accggtcttg agccggttaa tcgaatcggc catcgcggcg aacggcacgg
     5281 aattcaggtc gcaaccaacg tcgccgagca ggccggcgat gctgtcggga tagccctgga
     5341 ggaaggcctt ggccgcgtcg acgacgttgt tgacggtctc ctgggtcggg acgtcggcgg
     5401 caacttcctt gacgtactgg atctccttgc tgatgacttc attggtgcgc ggattggcct
     5461 cgcccgagac gagcgggatc gcgatcggca tgtcgctgaa atcgatctcc tcgtcgccgg
     5521 cgccgacgat cgcgcgcacg cgctccaggt gctgcttgta tttctccgtc agctcctggt
     5581 cctggagcag gttgccctgc tcgtcgaaca gcgcggtcat gcccagatca gccgagtggc
     5641 ggtagggctt ggaactagag gtggcgtgat tgatccagtc gtcattgatg ctccagttgc
     5701 gggtgtagcc ggtgtatttg gcgacggcca gccgcgtggc ggggtcctcg gggttcttga
     5761 tcgtgacgcc gggaacggtg gcgcgcagcg actggtagag ccggtcggcg ttggccaact
     5821 ggatgttgcg catgaagtag agccagtaga tgtcgcgcag gtcccgatgg agtccgggga
     5881 accgggcctc ggtctcctcg gccgactcgc tgtactgcga aagggtgccg atggcgtcga
     5941 tcgggctgtc cttctggctg atgaaccgcg ccttgatcgt gcgggcatgc ttttcaacgt
     6001 cgccgacgcc gccgcgcatg ttccagagcg accagccgca caggccgagc gtgagcagga
     6061 tcaccgcgac gctcccccag atggcggcga tggcgagctt gcggccggtc ttgagccgct
     6121 tctcgctgtg gatcaccatg ttgcgctcgg ggaagagctt ctcgacgtag aagtcgcgga
     6181 tgaagaaggc gcgcgagcgc tcgaagatct tctgcaggtg ttcgatgatc tcatcggccc
     6241 cctcgcccgc cgaacggagc atggcggcgc acgccttggc gatcggccgg ccggtctgca
     6301 tcccgctggt gaagtagaag ccgcggaaga acagcggggg gagatagtgc gtcttgacga
     6361 agatcgtgcg caggtagtcg ttgagcggtt cgcggatcga cttgaattcc tcggggaaga
     6421 cgtagagctt gtcgatttcg gtcaggctca ggtcctcgcc gaggatcttg ttgcgccact
     6481 tgtgcacgcg gtcatagatc tgctggaaca ctgagtcgaa cttgtcgggg tcataggcct
     6541 ggatgaacgg cccgtgcatc gaccagccga gcaactgacg ctgctcggtg gcgcccaggc
     6601 gcgagaagaa ttcggtgaag cccaggatca tgtcggactt ggtgatgagg atgaagacgg
     6661 ggaactggac ttcgagctcg cgctggatct gggtgagttt ctcgcggata ttctgcgcct
     6721 tggcttcgcg ttcctcgggc gagtcgccca gcaggctggt gcaggggatg gtcaggacga
     6781 cgccgttgat cgggcaggcg gggcgatatt tcttgagcag cttgaggaag ccggtccatt
     6841 cctcggagtc gggggcgttc ttctcctgga aggtgaagcg gccggcggtg tcgaggatga
     6901 tcgcctcgtt ggtgaaccac cagtcgcagt tgcgcgtgcc gccggcgccc gagagggcgt
     6961 cggtgccgac gggaaactcc agcccggaga acttgagcgt ggtgctcttg ccggattggg
     7021 gctcgccgat cagcatgtac cacggaaggt catagaggct gaggccggag gccttgagct
     7081 ggccgacggc ctggttccag cgctgggaga gctcgcccat cgccttgcgc tgttcggcct
     7141 tgcccttgcc cggcttgttg gcgtcggcct gcaggccctt gtcgaaggcg ctggccttct
     7201 tccggcggcg cagtttcagt cccttgttgt gcaggaagat gacgatgctg gcgaggatca
     7261 ggccgaccat caggatgctg gcgattttcc agccgatgat gcggccgacg atcgagatcg
     7321 cgccgatggc gaatgtaccg cccgccatga agcggtagag gaagggcatg tgggcgaggt
     7381 tctggatcat ggtacgaatc ggaccttcag cggagattca ttgaacggag ttcatcgact
     7441 cgcgagcgaa tgtcgatggc ggtgtcgtcg gtcgcggacc tcccttggtc aaatcgcggg
     7501 gatctcgggt gtcggggtcg gcgtgggcgt cggcgtcggg gtcggcgtcg gtgtgggcgt
     7561 gggcgacggc gtgggcgtcg cttcggccgg cgccgtggtt tcggccgcgg cggggagggc
     7621 cggcgcaacg accggtgcgg ccggcgtcgc ggccggcgcg ctcagcgtgg cgaaggggtc
     7681 gcgcgccggg tcgggcgtcg gcgtcgccgt ggcctccgtc tcgatcgcgg tttccgcctc
     7741 gtcggcctcg tcctcgacgt cggccccggc ggggcgcgcg tcctcgcgca tcgcctgggc
     7801 gatctcgcgg atcgcgcccg gaacgacgaa tcttttgtaa tagaagaacc cgccccacag
     7861 cacgagcagc agcgtgccgc agatgatcag gatgcgggtc aggttgacca gcggtttgta
     7921 aatgtccttg accccttcgg cgacgtagta cgcatcgggc gtgatgcgct tttcctcgtc
     7981 gctgacgcgg ccgggcagca tccggtagag ccggcgcttg agccggcgca gttcctcggc
     8041 gtcgtccttg aaccgcccga cgaaccccag gcacatgcag tgatagaaga tctcggccag
     8101 ttcctcctga tcgtcctcca gttcattgat catctcgaag aagcgttcgc cggcgatgcg
     8161 cgtcttgaag aactcgtatt cgaggatttc gtcttcccac tggctggagt aggcccagtt
     8221 ggcgttgatc aggatttcgt cggcggtgac gatgagggga tacttggccc gttcatagag
     8281 cttggcgagg cgggcatcgt cgcgcacgcg catttcgatc gattcgaaga tatcgagaag
     8341 ctgcgagcgc acctgataga tgtcggggat cagccccttg cgcacctggc ggcggaacgt
     8401 gacgagaaag agaaagagat cggaggcaac ttcggtcagg ttcatgggtc aattcaccgg
     8461 attgtggagt cgtcgggttg gcgacccgtg ccgcgatcgg cgcgcggggg gcgagtcgcg
     8521 gaggagccgt cgcgcggcgg acgccgcggg gcgcgggcgc tcagcgggcg gcggcgcggc
     8581 gtgtgcgcat cgagtcaatc cccctcgtat tcgggcttca acagaatgta cagatccagt
     8641 tcgagcgtct cgtcggccag gccgtaactg gccagcacct tggtctgcag caggctgtcc
     8701 cagaaacgcc cctcgcgcga catgcgatag aagtgatagt tgggccggtc gggcaggccg
     8761 atcggggtgc ggcgcacgcg gcgcagcatc agaccgggca ggcgccggtt gatcagcgtg
     8821 ggaaggtctt cgaccgagcc catcttgaga taggagacct gccggtcgat cgcgtcgtcg
     8881 tcctggtcgc tctcgatgca gaggtagaat tcgacctcgg gcaggagcca ttcgtcctcg
     8941 agcgcgacct cgcggcgatt ctcgaccggc tcgaaccggc gtttgatgaa cgtcgtgggc
     9001 aggatgttgt tgatcaggcg ctcgagcagg cgcagcagat ccagatagca ggcgccgagg
     9061 tcgtcgtggt tgtagagcgg cagctcgggc gccgcgcggc cctcgtcgaa gatcgccagg
     9121 tcgccggcca gccggcacag ttcgacatag acgtcgaacg ggtggaggtt gggcatgctc
     9181 gtccactggc gcaggaaggc gacgtagcgg ttggtgatgt tgagcttgag cattgcctcg
     9241 gggccgccga ccccctcgga gccgaaggcg atcttgcggc cggcaatctg actgatcagc
     9301 gagcggttct tcgccacgag ctgctggtag atatccttgc agtaatcgaa cagcgggggc
     9361 caggcctcca tgacgagaat cggcgggatg aagttgcgcg agaggatcgg cttgttttcc
     9421 tcgaagcccg agcgctcgat ccgcgcaatc tgcaacgttt catagccggt ggtgtcttcg
     9481 ccgcggaaga agatccgggg gctgtatttg cggaactcga tggcctggcc gttgctcccg
     9541 gtgttctcgt cgacaacttc gctgacgacc gccttgtagc ggcgatggta gatggaactc
     9601 tcctcggaga gcagcagcgt gttgggttcg cgttccatca gcagcggcag gccgatgtag
     9661 acgtcgagca cgccgccggc cgcatcgagc tccttcttga agctgcgcgg ctggaactcg
     9721 ccgttgtgcg gggcgatcag gcgcgtgccg tccttgagcc gcaggttgca ggcgcggatg
     9781 ttgaacgtga agccttcgag ctcggcaacg gagatgttga tctgctgaat gccataggcg
     9841 aagggccggg cgatgccgat ttcacgatgc agggcggcgc gctgactgcg gctcgcctgc
     9901 tgcagatggt gcgggcgaag gaacatcccc tcgcaccagt taatctccat gaattcactc
     9961 attgtttccc caaccgttca cgatggaaca gcgcgcggga gcgagtcccg cccgcgtcaa
    10021 agccgccatc tgcggacgat cccccgcccg gaagcctcct gcgccgcatc ccgcccggcg
    10081 acgggcggaa tcgcgaaggt gccagcgggt ggcccgtcgg ttcgaacgtc tcagtcgcgc
    10141 cggtcgcgat cgcgcgcgcg ccggtcgtcc tcgtggtcct cgcgaccacg tagatccttg
    10201 ggtcggccgt cgagccgttg caggctgccg tcgtggaccc agatatactc gtgcttgcgc
    10261 gggcgaggct tcatgtcctc gcgaaagacc agaatgggtt cctgcccccg gggcggattc
    10321 ggatcgggat tggtgaactc ggcgatgatc accatgaacc ggtcctgctc gtccttattg
    10381 ctgagcatga gcgtcgtgga tttctcgtcg agccactcct tatccttata agcggtgcgg
    10441 tacaggtcgc tgcggaacca gccggcgggg ccgatctcgc gggtgatctg gtagagctgg
    10501 tcctcatcct tgggatagac cacatcgacg gcgcaggggc gtccctcgtt gagcgcgtcg
    10561 gaccgataga acgtgatctg ctcggtcgga atgggcagac gggaacatcc catggccagc
    10621 ccgcaagccg ccagcatgag ccacaaagct tttctcatga ccttgttcct cccgcgaggg
    10681 aatccggtga agctgtgcca gatcagtcgt cagctaagcg cagttattcc atcacattgc
    10741 aagccatagg ggaaggattt tcagagggag aaaatgccga tgggaacgca attcggaggg
    10801 aggcgtccgg cttggcttgc cgcaagcgcg gccgccgaac ttaatgttgc gcgtcgccgg
    10861 ccgccatcgt ctccagccgg aactgctcgc ccgagacaat gcgctcgagg cggccgtcgc
    10921 ggatccagca gacccgatcg gacgcatgca gcattttcgg atcgtgcgtc gaacagacga
    10981 cggtgacgcc ctcgccggtg ttgagctcgc ggagcaactc gacgatctcg cggccggtgt
    11041 ggctgtccag attgccggtc ggttcgtcgg cgaggatgac tgtcggctgg ttggccaggg
    11101 cgcgggcgac ggcgacgcgc tgctgctggc cgccggagag ctcgaacggc ttgtgatgga
    11161 gccggtggcc gagcttgacg cgctccagaa tcgccgcagc gcgcccttcg gcctcctcct
    11221 gcgccgtgcc ggcgaaaatc atcggcagca tcacgttctg cagcgcgctc atcacgggca
    11281 ccaggttgaa ggtctggaag atgtagccga tcttgtggca gcggaaccag gcctgctggc
    11341 tttcggtcat gtcgaagatc gactgaccga gaaacaggac gcgcccctcg gtggggatat
    11401 cgagcgcgcc gatctgattg aacagcgtgg atttgcccga gcccgacggc cccatgatcg
    11461 acaggaactc cccctcgtaa atcgtcagat cgacgccgcg cagcgcccag acttcctcga
    11521 cttcggcgag ccggtagcgt ttggagacgc cgtggacttc gatgatcggg ttggcggtgg
    11581 cggcgggtcg agtcatggtt ggcctgcgcg gatcgtggag atgagtcagt ggaactcggc
    11641 gtgatcgaag tcgatttcgt cgggacgggc cgagcgggtg atgcgaccgt cctcgatcca
    11701 gaggatgcgg tcggagcgcc ggagcatctt caggtcatgc gtcgccgaaa cgatggtgac
    11761 gcccagttca tcacgcagac gcgccagcaa ttcgatcatt tcctcgccgg tcttctgatc
    11821 caggttggcc gtcggctcgt cggccagaac aatctcgggg ctgttggcca gcgcgcgcgc
    11881 gatggcaacg cgctgctgct ggccgcccga cagctcggcc ggcttgtgca ggatgcggtg
    11941 gccgaggccg acgagctgga gcaggtggat ccccttgtcg cgggcggcct ccccctccag
    12001 gccggcgtag gccatcggca gcgtcacgtt ctccaggcag gtcatcacct ggatcaggtt
    12061 gaacgtctga aagatgtagc cgatcttgcg gcagcgcagc caggccagct catcggtgtc
    12121 gaggtgggcg atatcgacct ggtcgatgta gaccttgccg gcggtcggct tctgcaaggc
    12181 gccgatcatg ttgaacagcg tggatttgcc cgagccggag gggcccatga tcgacaggta
    12241 gcagcggcgc ggaatctcga gcgtggcgcc gcgcagggcg tgcgtctcct gatcgccggt
    12301 gcggtagacc ttgcggacgc cgacggcctg gatcacccgt gagggaggcg cctgggccgg
    12361 cggttcaaac agcttgagaa ttttttcgcg attgagcgtc atgcgggcga ttgcccctcc
    12421 gatggatgtt catccctcat tcctcgtctc tcgtcctcgt cctcggtcat tttcaacgtg
    12481 cgcggaaact cccctcgccc gcttcttgcc cttgcgtgtc attcgtggtc cagtcccttt
    12541 cgtgtcttcc gtgatccaac ttgtcttccg gctcccctcc ggcatcttac acattggacc
    12601 tgagcgcggc ggcgggcgtc atccgcgcgg caacggcggc gggatagagc gcggcgacga
    12661 ccgacagcag gatgccgcag accagcgtgc cgccgatcac gccggccagc gccagataat
    12721 tgatcgaagc cagcacgagc gcgggcccgt acgacaccgc ttgcagcgcg atggcgaaca
    12781 ggcagcccag cagcgccccg gccagaccgc cgcaaaaccc catgaatcca gattcgatca
    12841 ggaacatccg gcggatgaaa ctcgaccgcg cgccgaggca tttcatcgtg ccgatctcgc
    12901 gaaaccgctc ggtgaccgac atcagcatcg cgttgctgat ccccatcacc gtcaccagca
    12961 gcgcgatgct gaggatccag acgagccgat agcgttcgcg gagctgctcc tgttccaggc
    13021 gcgcctgttc ctccggccgg atctcgcgcg ccagcgggac ccagcgcgcc gtaagctgag
    13081 gtggccgggc ggcggcggac cgcgtcggcg caaccaccga ccgcgacgcc agcgcggtcc
    13141 acggaacggc gtcgaacggg ccggcgccgg cgaggatcac ggcggcggca ttgcccagcg
    13201 cttcggaggt cgcggcatcc cccgcgatca cctcagcgcg gcggcgctgg agttgctcga
    13261 gcagtttcgt ttcctccgcc gccagcgcgc cggcgctgta gatcacgatc cggcggtcgc
    13321 gcaccggtcc ggtttcggct tcgaggaaac tgagcatgcg gccggcttcg gcgcgcagcc
    13381 gatcctcctc gcgcacgccg tggcggatga gctggctcgt ccacgcggcc gacagaaacg
    13441 cgatgccgag caggacgccg gtgatcgtca cgagcgagcg cccgaagcgg atgcgaatgc
    13501 cctgcgagac gatctggacg gcgacgcgcg gcggcaggtc gatctggctg gggatcgtgg
    13561 tgggctcgcg gttcagcatc agaacggcgc cgaggagatc gcggatttaa tcagcatcag
    13621 ggccattgtc atcatgctga tcaggccgac gccggtgtaa tagccggcga gcagcgcggg
    13681 ggccaggcgc aggaaattga cctggccgaa gcgtttctgg aaatagaagc ggccgaggag
    13741 cgcgccgacg acttcgagaa tcatgaaatg ggggagttgg ccgaggccgc ggatcacgcc
    13801 gtagatcagc atgatcggca ggcccagcag actcaggccg gcgtagagcg cgatgcagcc
    13861 gacgaacccg ctgccgatga cccacggatg gatcgcctgc atgaattcgc tgtcggcgac
    13921 attcgacccg ccggtttcgc ccggcccgac aaacgtcgag gaatacagca gaacctgatt
    13981 ctttgcctgg agctcccagt tgacctgcgc ggcggggaac agatctgagg ggatcgggtc
    14041 ggagtgccag atgaagctcc agtagacgag cgagaggacg agcaggatcg ggatggcgac
    14101 cagttcggtc ttgagcagcg agggaaagcg cacgccggtc agttcattca cccggaaggc
    14161 ctgggccatg tagccgtagt tttcgaccgg aaccggggcg agccagatgt cgatgccgcg
    14221 cgccccggag aggatgaacg cggtttcctt gacgaagggg atctcgacgt tctgccccga
    14281 gatgccgagc agtcgcgcat tcacatacga aatcagcggc gtgtaggcga acgtgaagaa
    14341 aatcaggaag aagaaaatgc tgaacgtctt ggggagcagg aaccagcaga gggcgacgac
    14401 ggcggaggcg gcgccgatgt agaggaccag cgcgatccag agcggatagt cgccccgccc
    14461 cgccggatga tcctggaaca gattgcgcgg cgcggcgccg gccgcccgct ggcgacgcgc
    14521 ctcgcggacg cggacgacca cgtcgcgcag cgtggcgtag aagcagacga cggcgatgcc
    14581 cagtgccgtc ccaatcgtca ggctcatcca gaagtcgatg tgattggcga acgtcgtgtt
    14641 gaccgtatcc atgccgggct gccagcgcgt cagcacgccg gcatggtaga ggatcggatt
    14701 gatcaggatc gtcgtcgcga tggccaggaa cgagccgatg acgacccaga atggcagcac
    14761 gaagccgagg aagacgatgc ccaggtcgaa cgtcaggccg gtcggcgtgg ccggcaggat
    14821 cccctgcgtg aggaccgtcg tatcgacgaa cggctgcggg atgaggaaga acggcttggc
    14881 caggaacagg ctcgagacgc cggggatgcc gatctggagc aggccgaaac cgaggccgaa
    14941 aatggcgccg agcgaaaaga tgcgccagcg gctggtcttg ccgcgcgtct gtttgccgcg
    15001 cgggtcgggg tcgtcggcgg tgggcgcctc gccctcggcc tcggccagcg ccatcgcccc
    15061 ctgggcggcg atcggcgcga gcgggaacgg cagccgctcg atgtcggacg tcagccggaa
    15121 gaaaaagtag ccgagcgtgt agcgcttgat caggccgatg accatcatga acgcaatgat
    15181 caggatcggg acgagccagt cgcgatgcca gagggtgcgt tcggccagcg cgtcgctgcc
    15241 ggggggcggg acgaaccact cggggaacga gagcgccatg ccggcgtcgc gcgcggcgtc
    15301 gctcagcacg aggtacgcgc ggtagacgag ctgacccatc gggccgccgg ggaacaggat
    15361 gtgcgcggcc atcatcacgt tggccgcatg cagcaggacg acgagctgct ggctggcgag
    15421 cggcttgagc gcccgccggg cgatctccat gaacaggatc acggtgaccc aggcggcggc
    15481 ggggccgagg ttgccgccgg tcatcaggcc caggtagatc gccccgggca tcatcaccag
    15541 gccgcagaag aagatgccga cgacggtcgt cgcgctgaac ccttcgcgga aatgcgcggg
    15601 cgtctccagc agggagcggt aaagctcgac ttcccggtcg tgggtatcgc gcgccatcag
    15661 gcgtgctcca gtccgaggtt cgtcaggatg tggtcgcgcg cctcgtcgaa caggttccgc
    15721 cgatcgctct catcgacggc gcgccagtgg aggaagtgcc ggcgcagatc ggcgacgaag
    15781 gccttgttca ggcggagcca tgcctcgcgc gtgccggtca ggcgctccag gcggacgccg
    15841 gccttgaact cgcccgtttc cggatcgggc gtcatcacga tctcgagccg ctgcgagacg
    15901 cccaggtcga acggcttggg ccagatcgtc gcgcgaatgg cgggaaggta atcatcgtcg
    15961 ccggccgcgg acggctgctc gacgccgagc tcggggacgg aggcatggaa cctgccccct
    16021 ccgccttcgc cgtgatcggc cagccagcgc tcgcagaagg caaggacggc gatccggtcg
    16081 cggtggtcga agttgaacgg cagatcgaac cggagatcgt cgccgtcggg ctcggggagc
    16141 gaccagccgg cctcctccgc cgggcgggcg atctccatcg ccgcgcgcgc ggggaaccac
    16201 gtcgagatga agacggcggc ggccaccgcc agcgacgcgt agatcgtatt gatcgacgtg
    16261 tacgtcatgt tgaggccgcc ggtcagatcc agttccgtca ggatgcggcc gacgccctgg
    16321 ctgagcaggt agcccagcac cgtcccgacg acggaataga cgaacgcctc ggcgatgaac
    16381 atgaagaaga cgtagcgcgg ggcgatgccg acggcgttgt agacgaagat ctcgtcgcgc
    16441 cgttcgtaga ccgagccctt catcgtgttg agcacggtca acgcggccag gatgagcggg
    16501 atcagcagct cgatcaggcc ttcgaacgag gcctcgcgcg cgcggcggcc gcgccaggcg
    16561 accccttcga gaccatagaa cgcggtcttg ccggtctgct cgaggtaatc gtcgatttcg
    16621 gcgcgcgcct cggcgtagcc ggcctggggc attgcgacgg cgaccgacac gatggcggga
    16681 atgccgttgg ggacctgcag gttcagttcg cgctgcgggg cgaggatgat gcgcgccggg
    16741 tcgatgcgcg ggtcggcgtc ggtcgcgaca acttccccct cctgaagctg gatatgggcc
    16801 atcgcctcga tgtcgtaggg gagcaggtcg cggccgtcca ggtcgcgcat ctcggcgagc
    16861 gagcgggatt cgaagatccc ccggacgcgg aaggcccgcc cgttgatctg aacctgcacg
    16921 tcccccgtat tgacctccgc cgccgtcagg ccgagccgct cggccatgcg gtcgggcagg
    16981 atcgccgcgg gcagctccgc cgacgcttca tccgcggggg tgaaccagcc ccggtcggtc
    17041 agcaggcgga tctggtcgcg cagcggatcg ccggcgctca tctgcatcag cgaatcggcc
    17101 tgagcggcac ggcggcggcc atcggtctcg taaagcacga ccaactcggg gttgtagccc
    17161 tgggtatcct gccagttctg ctggccgaga tacatgcggc ggggattgac ctggtagcgg
    17221 tcgccgtagc gttcggtgat cgcgaacaat tcggcgtcgc tgatgcgctt gaagtgttcg
    17281 ttcttgatca gaatgccctg gtagccggcc ttgcccaggg cagtcgcttc atcgacgacg
    17341 tcgctgtgga ccgaggtgaa cgcgatcatc gcgaacgtga tcagcgtcag cgtgccgcag
    17401 gtcagcccgg tgcgtacctt gcgccggtgc atgttgttga ggccgagcat gaaggccgag
    17461 ccgatgacgc ccagccggtt gatctgggcg gcctcgacct gcccgcgccg cttcttgagc
    17521 tcctccaggt tctccttgaa cttgccggag aagagaatgg tcatccccgt cgaaatgagg
    17581 atcacgacga acccgagcag gatcatcacc gaggagcgga ccatcccgaa ggcggggtgc
    17641 aggacccgca gcaagccgaa ggcaaccagg aagatgatga tctgggcggc gatctgcttg
    17701 cgcacgtcgc tgtagccgaa aaccattttt tcgaaaaaaa acgtgaacgg gacgagcagc
    17761 gcgagatacc agatgatgcc cagcaccgcc tcgaacagcg aacgccgcag gaccgggtgg
    17821 ttcagcatcg cgtaggtgac cgcctcgcgc gcgagcagga tcttctcgcg ccagggcagc
    17881 tcgggcgcgg cgctttcggc cagcagcccg cgcgccttgg cgtggtagtc gttcgtgcgc
    17941 gggtcggcca tgcggtggcg gttctgaacc tcgaggcgcc gcccgttgag gaacgtcatc
    18001 gactcgccca tctcgaacgg caggccctgc atgaccggcg tcgcggcggc aaggtagccg
    18061 ggcccgtcga tttcgcggtc gggatcgggg acgaagtcgc tcggctcgcc cgcgcgcggg
    18121 acgcccagca ggaagccgcg gatctccttg accagctcgt tttcgggcga gcccgactcc
    18181 agcgcggcgt agaaccgcgc ctcggggtcg agccacgcca tgcgcatcgc attggtttca
    18241 tacaggcagc gcttgcggaa ttcggccagg ccgtcgcgcg aaaacagccg cagcccggag
    18301 taatcgcgca gcgtctgcgg attggtcaga tcgaagaacg ccaccggcgc ggcgcggaag
    18361 cagaccagcg tgatgttgtc gaccttggcg tcgaaccagt tgagcttgac cgacttgtag
    18421 agccgctggc cgtcctcccc ctcgtccttc atccagcgga tgaatccgtt gcggtcgtgc
    18481 cacgccgcca gcgggctgta gccgttggcc cacacgttct ggctgaccca gaaatccgaa
    18541 gcactgttga ccagttcata gcgcccgtag ggatcggtca tgatcaggat gtgatcgtaa
    18601 tagccgggga aggcgtaggt ttcccagttg agataggtgc ggttggccag cacggcgccg
    18661 gcgagcgggt aatcggggac gagcgactgg ccgatgccgt cggccagcac gcgcccgccg
    18721 aacgtgcgct tgagccagcc cacctgaatc gcggcgagct tgccgttgcc atgcgcgatc
    18781 gacaacaggt tctcgccgac gaccgccagg ctgtgccgca gcgacgcaac gtcgtgcatg
    18841 aacggccggt cggtcggatc ggcccagtgc tcatacgact gctggcggtc gaagttgagc
    18901 agtgtgaagt tcaggtagcc gaaccgcatc cacatcaccg acgagcggtc ggagaccggc
    18961 tgcgtgttgc gatccacgtc ctgattgtgg tacttggcca ggcgcggaat ttcgagctcg
    19021 cggcggaggt cggcgttgcc ggtgaacgcc gccagccgct ggcgcgccga gacgaacagg
    19081 ctggccatcg cgcgcatctg ggcgctgacc gaccagtcgc cgttgctgaa cgtcagcact
    19141 tcctcgcgcc ggtcggcgtg gaacgccggc accagcttgt tgtcgaagat gatgaactgc
    19201 tgatagggat tgagcgcggc gagaacgtcg agatcgagcg tgagctgccg ccggcgccgc
    19261 tgatggtaag cgcgcagttc ctcgaatcgg gcgcgccagc gccggcgcag atcgtgctgg
    19321 ttgagaaagt cgcccaggcc cgactcggcg gcgaagaggt tttcgagccg ccggccgccg
    19381 acggcgacgg cgcggtcgta acgccggcgc gcctcctgga acgcggcgaa ctcgggcccg
    19441 tgcagatttt cgcccgtcgt cagaaagacg agcttggcct ggagctgggc ttcgtattgc
    19501 tcgaacgcca gcgtgtcgag gacgtagtcg acctggtcgc gcaggaaggc ggcgtcgtcc
    19561 tcgtccagtt gcgcgagcgc ggcgagcgtc ggccccggct cgctcagaaa gccgtcgttc
    19621 tcgaacagcg caaggatccg ttcggtccgg cgcagggcgg cctcgttctc ctcgtggcgt
    19681 tcgcgccagg gttgcagccg ggcggcgcgg gcgccggcct gcgcgtcggc cccggcctcc
    19741 gccgcgccca gcccgaggat ctcgcgcacg gggttgcgcg tcgccttgta gatgttctca
    19801 tcaagcagcc ggagaaggtt ttcatcgccg gcgcgcgcca tcatctgcgc gccgcaggcg
    19861 atgaagatca catcgcgcac aagcgaatcg cggtaggcgg tgaagccctc gagcagcgcc
    19921 agttgcgcgg cgggcgagat cgcctggatc acgcccggcg cccggtcggg tagaatcgag
    19981 gccgcatcgt aattggacag gatcaccagc gcgtcggtgt tggggcgctc gcgtgatgtc
    20041 cccggcgccc gcagaatgcc gaccagcgac gtgttgcgcg tctcctcgaa acggacgttc
    20101 acccgcagcg tgacccggcg gccggcgtaa tcaaagatcg ccggcgtggc cgccacgcgc
    20161 acgaagttca ccggcatgct cgaaaccatc ccgcccttgg acggcgttcc cttctgccac
    20221 gggacgacgc gctccaaggg gacctggtcg agcccctcgg gatggctgac aatcagcgcg
    20281 cgcaggccga gctgggcata gcgcccccag gcaaattcgt aatcgggcga ataccggccc
    20341 ggcgaggcgt caaccagacc gatgcagtcg tcgaaccgct cgcgggtacg gaggacttcc
    20401 ggcgtgagga ccagcagttc gccggtgacg ccttcatccg gggtcgcgac cggctgcaag
    20461 tgattcggca tgaacgggaa gatttcgacg tcgggcagcg tcgtcccctc gtcctcggcc
    20521 agcgcgatct cgcggcggat cgtgcggggg acgactgtcc agttctcctg ttcgtgcagt
    20581 tcaagcccgg ccgcgaggaa cgcatcgcgg atcagcgcct cggcggcgta gacgcccggc
    20641 tgccccatga accgcgatcc gaggccgacg atcgcctcct gacgcgcctg aacggcgcgc
    20701 cagtcgaccg cctgctgcat cggtcgcgga tcgtagctgt aatcggggcg cggcggctcc
    20761 aggtaggggc gcggggcgaa aacgcgcagg ccgagcagcc agaccgccgc cgccgcgacg
    20821 aggaagaata gcgccccgag cgcggagaca tggcgccggc cggcggcgcc gcggcgccga
    20881 tgcgatacga ttggaaagtg gcgcggcggc cggcgcatca gaagagcaac ctcgcccgga
    20941 ccaacgtcgg atggaagaag tagagatacg tattgatcgc gccgaagatc gcgtaggccg
    21001 ccaccgccgc caggaacacg ccgatgaaga acggcatcag ccgcgtgcgg acggtgacgg
    21061 cgccgcccag cttgagcgtg acgacgcgga tcgcccacgc cacgagcagg ctcccccaga
    21121 cgtactccag catcggcatc gagctgacaa cgaacccgac ggggtggaac cagaaccccg
    21181 aaaagatctg ccgcagcacc gtcagcacga acgtaacccc cgcggcgaac acgtagcccc
    21241 acgtcggtgg atcgatcccg gtgtcgggtg cgggcatggc cgggttcacc ttggccagct
    21301 ccagattgta ttcgttcacc agatggccct tgtccatgta cgggtagcga tgggtgaagt
    21361 tgtcgccgcc gatggcgtag cccagcgaga ggaagatcca gccgcccaga aagatcccgc
    21421 cgacgacgcc gagcagcgcc gcgccggtga tggcgcgggg cgggagccgg tagcggcggc
    21481 cgagttcgag cagttcaacc tgcatgccgg ggataaggaa gaagaggtgc cggaaaatga
    21541 tccagttcgc gaagctgacg aacagcacgc cggccggacc gaagaattcc atcccgccgg
    21601 cgatggccac cagcttgctc agcgccaccg gcatgaagat ccccatcggg gtgccgcatt
    21661 cggcgcggat cttcgccgag accaggccga tcagcagcag cgcggcgaaa aacacgagca
    21721 tcgccggaac ggccagaccg acccaccagg cccacagcgc gatgccgccg acgctgacgg
    21781 cgatgccggc cagcgcggcg ggatacgaca gcgcctcgcc gcgccatgcc tccttgcggc
    21841 cgcgcacggc ggcgctcacc acgcgcgcca ggtgcttgcg cgtgaagaac aggatcagca
    21901 cgccgtaggt caggaaggcg ccaacctgct gctggaagac gaacgggtat tcggccgtca
    21961 gggccaggcc ggtggcctcg ccgaaccagt actggcagcg gaaaaggaaa aagccgacga
    22021 tcaggctgag caggacgctc gactcgatga aaatcgcgat gccgacgtag aacgcgaaga
    22081 cggcgaacgt gacattgcgg acctcctgac cgcgccacat gttcccccag ccggggtcgg
    22141 tgaagtaggg cgtcagttcg accgcgatgc gcatgtcggg aacgctcgtg ttgaactgat
    22201 gccagccctt catcaggcac cagaagaggg tgacggcgaa gccgagccac atcagccggt
    22261 tgcgccagat cggcgccagc gcgtggtcgg acggcggggc gtcgggttcg ccgacgatcg
    22321 cggtcgggat gcgcgtcagc ggcagcggat agcgctcgct ctcgatccat tgcgagcgca
    22381 tcagcacggc aaggcagaac gtgccgagca acacgagcag ggcgatgccg gaccaggcga
    22441 cgatcggcgc gagccactcg cgccagggga tgtagcccgc cagcaggaaa cgcaggccgg
    22501 cgacgctcca caggttgtcg gggcggacga gcaactcggt gcggggcgat tcgcccagct
    22561 cggcaaaacg cgaggcgttg atcacctccc ggccgtaata gatcgagtgc agggcgccga
    22621 cgctgatcag ttcgacatcg gccagttcga gccgtccgcg gccttcgagc gcgagctcca
    22681 gatcgacatg gcgctgaacg tcgcgcggca ccgtgaggcc gtacatcccc cggcgcagga
    22741 agcccagcgg atgaaggtag ctgacctcgc cggccatgcg ggcggcgaaa gcctcggcgc
    22801 cgaactcggc gcggtcgtcg taatacagcc ggcaggcgta ctgcgcctcg gggcccagct
    22861 cgtgcggccg cagcagcagc gtgaccaggt agggttcttc ggggatgagc tggtggaacg
    22921 gatcgccggc gcccgcgggc gcggccagcg ccacgcgcat ccgtacccag gagacgtcct
    22981 ccggcgactg attgaccaga acgggccgtt cctcggaagg cgcgtgctcc agctcgatcg
    23041 tctcccatag aaccgtcccg ctggtgacga gatcggcccg gcgggcgggg gtcagcgcgc
    23101 cggcaagcag gttgcgcccg tgcggccaga gtttcttcgg gtaggcgtcg taggcgtcga
    23161 agtcggccgt cttggggatc gcgccgatcg cgccgatcat ataggaccag aaccccgacg
    23221 acatgagcgg cacggccatc atcaggacga agaacagcgc aatgagctcg ccgcgcccga
    23281 gcaggcgcac ccgcaggaga cgccaggcgg cgccggtcag caccaccagc ccgaggacga
    23341 cgaccacggc ggggatgggg agggcgtggg cccccaccat gtcgcccccg gcctcgatca
    23401 gttgaaagaa ctgcaccatc agaccgacca gcagcaggcc gaagagggct gccagcatgg
    23461 cgcgcgcggc cgaaccgccg gtctgtcgcg gtgcagggtt caacgggcct tcccccgggc
    23521 ggcgggcgct cccggcgggg gaggcgccgc ggcccgcgat cacgacttga gcatgtcgtg
    23581 ttccttggtg aagtcaatca gttcgcgcag cgtcgtcgtg gccaggcaga cgctcgtgtc
    23641 cgccgctccg tagtagaccc gcagttcgtc ggtcttctcc tcgtggatgc aggcgacggg
    23701 gaacgtcacg ttgggcacgc tgcccaccat ctcgtacggc tcggtggggg cgaggatgta
    23761 gcgccgcgtg cgatgcagca ccttccacgg ctggtccagg tcgagaatcg cgcagccgac
    23821 gctgtagaca aaaccgttac aggaggtcag cacgccgtga tagatcatca cccagccttc
    23881 gtcggtttcg atcggtacgg ggccggcgcc gattttcgtg ctctgccagc cgccggcggt
    23941 ccccatcaca aagcggtggc tcccccagtg tatcagatcg ggcgactgcg acaggaagat
    24001 atcgccgaac ggggtgtgac cgttgtcgct ggggcggtgc agcatcacat agcggccgtt
    24061 gatcttgcgc gggaagagaa cggcgttgcg gttgaacggc gggaggacca cctccagcag
    24121 ctcgaatttt tcgaaatcgt cggttacgcc catcgcgatg caggggccgt ggtagccgtg
    24181 gcaccaggtg atgtagtagc gcccctcgag cggggtgatg cgcgggtcgt aggcgtaaac
    24241 tatctgctcc agttcggagt cccggcactg ccactgaacg ggccgcggat cgatctccca
    24301 gtgcagcccg tcggcgctgc gcccgggatg gatcagcatg tcgcggttct gattatcgac
    24361 gcggaacatg ccgcgatacg cgtcgccgaa cctgacgacg gcgctgttga ggatgctgtc
    24421 ggagtcgggc agcgcattgc gcgggatgat cggattgccg ggatagcgct tgagcaggct
    24481 ctcggcgccg atcaagccct ggcgcggggc ggcttcagtg gcaacggaca tggggtcctc
    24541 tccttgaacg aggtggtgat gtcgatccat cgggtcgacg caaggcggcc gcgtcggccc
    24601 ggcatcgcca ggctcggcgc gccggcgcca gccggctggg aatcagggac gacgcacggt
    24661 gggccggcca tcgtcagcgg agataccggt tgcgtccggc gagaccggag gcggcgcccg
    24721 ccagcgccgg tcgccgcggc gggggcggcg ccaggctgtc cccttcgtgg atgagcgggt
    24781 tgctcaggat ctgcgaaaat tcggcgcgcc ggcgggtccg ctggacctgc agcagatcga
    24841 agcaggcgtt gagcatcgcg ttcatatcat attcgaggca ggtcatcgtc gcgccggatt
    24901 tggtgcccat cggcccggcg ccgtggccgg tgacgctcag atcctccggg acgcggatcc
    24961 ccatcatcgt ggcgacctgg acgatgtgga aatcgacgcc gccccacatg cagatgatcg
    25021 cggtggcgtc gcgaaactcc tccagaaacg tgcgcaggcc ggccagatcc ccctcggcga
    25081 tggtcatcga gcgccggcag gcttcctcgt tcacccgcag ccccgcttcg ttcatcgcgc
    25141 gagcgtggcc gtccagccgg ctttgcgttt cccactccgg atcggtgggg ttgcagaagc
    25201 agatgatgtt gcggtggccg acctcgatga tccggcgggt caacttatag aagccgagca
    25261 tcgtatcgcc ggcgacggtc ggatggcatt cctgactggc gccgccgatg tgaacgaacg
    25321 gcagacggtc gggcggcagt tcgaacgcgg cgggatgatg aaacggattg agcgcgatga
    25381 cgccgatcac cttgtccgag aagacgcccc ccacctgatc gacgcgcgac cagtcgtcgc
    25441 tgggccgcag atacttcacc tcgaccgcga agttgcgctg ctcggccatc tcgatcacgc
    25501 gccccagacg atactgcgtg aacggcggcg tcttccagtt gccctgctcc tcctcgagca
    25561 tcagcacgat gccgatcgag ccgagcggct tgcgcccctg cggcgcgagc gactccagat
    25621 aggtgcccga gcgcgagctc tgacgcaggt agccctcctt gcgcaggctc tcgaacgcct
    25681 cctggatggg ccagcggctc aggccgctct ggcgggcgag cgtggcgacg ctgggcagac
    25741 gctcgccgac ctcccagtgc ccgccgtgga tttcctcgag caggatctcg cggacctggc
    25801 tggtcagcgt cagcccctgg tcggggggga aaatgggacg tccgaaaagc tggggatgtt
    25861 ctttagacat ggttgccgat ccagtggggc cgccgcgcaa atgggcggcg gggtcatgac
    25921 tggagcgatt gtaactgtca tgccactgcc atgtcaattt gaaattgaca gggattgacc
    25981 tgtcatacag gcatgaaacc cggcagtggc ccgttccgcg aacgtggcgg gcctttatcg
    26041 agcttttccc caaaaacagg gatcgatccg gaaaaccggc ggcctcgcgg cgcccgggag
    26101 cccgccatgt cagtaccgtg tcagtccacg acaacctgca ttttcctgtt gacatggacc
    26161 tgtcagattg atagggtggc aaatggtc
//
LOCUS       DAOPCO010000030        25131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2128_length_25131_cov_6.327405, whole genome shotgun sequence.
ACCESSION   DAOPCO010000030 DAOPCO010000000
VERSION     DAOPCO010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 25131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25131
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2128_length_25131_cov_6.327405"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..238)
                     /locus_tag="PLS90_04045"
     CDS             complement(53..238)
                     /locus_tag="PLS90_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01608.1"
                     /translation="MTQPSRTGFTLIELLIVVAIIAILAAIAVPNFLEAQVRAKVTRV
                     HADFRTLATALEAYQVE"
     gene            complement(251..862)
                     /locus_tag="PLS90_04050"
     CDS             complement(251..862)
                     /locus_tag="PLS90_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01609.1"
                     /translation="MLRRPPRTGFTLIELLIVVAIIAILAAIAVPNFLEAQVRAKVTR
                     VHADFRTLATALEAYQVDNAHYPPDRDEMTLAGVPVPNGYAFNFLTTPVAYLSSIPTD
                     KFFDVEMWPTEPPRYWYQGEGWRNHPVVKARAIHGFRGKWIASSHGPDLFCDFGETAL
                     LAGEVPFMTVATNGTTVIRRYPEMIYDPTNGTVSSGDLVRWGP"
     gene            1366..2475
                     /locus_tag="PLS90_04055"
     CDS             1366..2475
                     /locus_tag="PLS90_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK01610.1"
                     /translation="MSRTNLTMADIARICGVARSTVHAVLKGKPGVSARTRAKVLDAV
                     ARFDYSPCPIAKSLARKKSGLVGVVIRDLANPFYDQFLKHMDHVLKDLRVITFNTRHD
                     IDKEIHALRTLAGYRADGLIIAPVQPERAADHLGDWVERGGALVSLDRLPGIECSYVE
                     FRNFDIGRMTTEYAIRLGHRAICMLAGPPRDDARDDERVAGFKACLREQGLDPAECTV
                     QHVGWRWEGGFHAASEFFSGARRCPSLFICNNDYVAGGVYKAAYVHRLRIPDQVSVIG
                     CDDIGLAAVLGPSLTTVRLNARGIGEGVARMLLRQMNQGSAFQPRSIAVAPMLVERES
                     VLNLTATDSAEACCQACRERADAAQREEAACLSKG"
     gene            2899..6105
                     /locus_tag="PLS90_04060"
     CDS             2899..6105
                     /locus_tag="PLS90_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase N-terminal Ig-like domain-containing
                     protein"
                     /protein_id="HPK01611.1"
                     /translation="MRHSKPRMRASHRIGCALLILFAAATARAGQPVLFSEDFEGRPA
                     GEALHGTPLNNGLGGQVAATWWDPKGLAHAGADEQGGRGEIAPGGWDHFKTLIDYELD
                     PDEAYAFELELRPPGGVLQPTPWNPHSGLVILSRKDTGMMASGVVLRIQGPDPSGKDP
                     NRFTLVYQREEGNYFDDPALIGDIVPEDYNAAGVIPTRIELSGRGTAAHPLVGRLLLN
                     GRERGSFVIPDLFRGGRYIGLVTQSSPKEGAAPGLVDNVKLERLEAPVFNPAPRVAIA
                     PVLLPVAMPELELTAFVRDDGPTDRLRLGWEMIDGPAAATFSDSGRTTTRVTFAAPGL
                     YTLGFSAFDGEHRAEERLSLVVAPPPSEAPLAEAGFSEGEPLAAKDALPGQVLTRPLP
                     LAADAGHGLRARWRMAPPSAAGWGGSDRIIVALTDAEATVGREIHLVPGDAEAAGPAA
                     AEPAGDRPSPITLPIAAAARDELLLQLDLAGTGTPRDPLRGRLLVNQERKLDFDAGPL
                     AADVRLSVAVVTEGGAPVQLDDFELAALRNPFVVEDFMLLYSTLGYDSRASKRALVRL
                     HTRSDAESLDAEASSWRLLNQEGEPVLSGPLMPLPKTFGCLLWEAEFSSWTTPGTYSL
                     EVTLVDRDAGELAPLVSEPFLIEPRLHYQRTFEGIALLNAEARRATEDVGGGYYDCNS
                     AMGEAYSHGAFLAGLSKAYQRFADDLTLTDRDRLREAANRAADYLMLLHNAQTGEIIC
                     QHPNRVQGAESGDGPHNTFEGIWGLLNYAVVFKDLEPARALDAFNRSRKSYQWLGNQG
                     IYYPDQASAFMALIHQYQPTYPVQETLFRTLNDFLREFNPRQWGRSCGRCIPYFEGLW
                     YCLRTFPDHPDRSRWESLARDFNQQWYQEMMQRNAFHILPQGGAREWDNTEDVPPEIY
                     PPYNFYASTQFGTMATDAILLARITGDDSLERMATGCLNWITGLHPGVPRNSTLPAAT
                     GRRLTAAALIMNLPGARHIRTWTYWGWEQPEIEYLSIVNGLCIDDGDWYYSTREWRSG
                     ETFIKHDGAYLYAMALYDQYLEENRSGVTSLAQLAQYE"
     gene            6144..7025
                     /locus_tag="PLS90_04065"
     CDS             6144..7025
                     /locus_tag="PLS90_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK01612.1"
                     /translation="MARKHYRNEIEGYLFLAPQLLGLVLWLGIPMLAAFALSFTHWDL
                     FGRPEFVGLRNYINLLFHDRRMWATLFNTAYFTAGTVTLGTTLALLLAAAVHQVSFGQ
                     TLFKSIYFLPVICSMTAIAALWRWLFSGDFGLLNLYLERIGISAPDWLNDTRFAMPAV
                     ILMSVWKGVGFPMIIFLAGLHDIPAVYYEAAEIDGADRRRQFLHVTLPQLSPIILFVV
                     VTSVIGCFQTFDQIYMMTSGGPMGSTRTIAFYLFENAFVFYKFGNACAIAYVLFVLLG
                     GFTHFSIRLTQRWVHYQ"
     gene            7198..8043
                     /locus_tag="PLS90_04070"
     CDS             7198..8043
                     /locus_tag="PLS90_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK01613.1"
                     /translation="MRRGSHKLSRRLFLLLLYAVLTAGAVVMVAPFLWMVSTSLKAPH
                     ETFMLPPIWIPEDFRFENYAEVFDRLPFFRFLLNTATIAGLVTFGVLLSCSLTAFALS
                     RLRFRGRDLVFYILLATMIIPAPVTLVPIFAIMRRLEWTNTFLPLVVPAFFGGAFPVF
                     LLRQFFMRIPRDLDEAAVMDGCSAFGIYWRVILPLGKPALATIGLISFMGAWNDFFGP
                     LIYLYSIKKQTLTLGLATFQGYYATDWHLLMAATVIVTLPILLAFLFSQRFFVEGITM
                     TGIKG"
     gene            8120..9373
                     /locus_tag="PLS90_04075"
     CDS             8120..9373
                     /locus_tag="PLS90_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPK01614.1"
                     /translation="MAGVVARLLAGVAVLLVAACGGEDEDRRIELRWGLWGTPEAVQN
                     QRRFARDFMRLHPNVRVEIEPVAGDVTVAFSMRLAGGDAPDVLYAGNWFLFEFADKGT
                     LLPLDDLIARDGIDMGQFFDSALSGIQWDGATYALPEAINVEMLFYNQDIFDRYGVPY
                     PEPGWTWDDYLEKARRLTQDTDGDGRTDIWGCYVWTWIPGFLPFMLQNESNMFDDDFR
                     RFTMDDPRAIEALQFIQDLIFRYRVAPGRQALQSQLSIDMFASGRIAMGSFGYWGVRE
                     LQGAPFRWDVAELPRRRTTGNVVHVHSFAINRETEHPEEAWELIKYLTLDEPAREVVY
                     LIPAQKRIAAEMIRAGNEGMPANAEAILATATDPNTVSPPFGPRFGEYSSVLGLELDG
                     VWLGKVSVEEAVRKAKPQIELILSE"
     gene            complement(9535..10596)
                     /locus_tag="PLS90_04080"
     CDS             complement(9535..10596)
                     /locus_tag="PLS90_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK01615.1"
                     /translation="MATNEKKLTMEDIAKICGVARSTVHAVFTNKPWVTEKTRNKVMD
                     VVQKLNYSPCPIAKTLASGLSGLVGVVIRDLTNPFYDQFLERVEDLLKDYVIVYFNTR
                     HDPRREARAVETLMGYRADGLIIAPARPELGTTHLTNWVRQGRALVSMDRLTGTDSNF
                     VDLNDRHIGYMAAEHLFSHGHRRVCFFAGPRQREHHDDERVVGFKMCMLDYGIDPYDC
                     DTIRVGLGWENGYRAASKLLAHNRDAFTAIICGNDYVAGGVYKAAYGHGVRIPDDLSV
                     IGCDDIDLASVLGPSLTTINLNVRDIGEKVALTLLEQIRGGEDFKCVSIPVSPVLVER
                     ESVKSLSRIAPRQARARGY"
     gene            complement(10861..12204)
                     /locus_tag="PLS90_04085"
     CDS             complement(10861..12204)
                     /locus_tag="PLS90_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPK01616.1"
                     /translation="MSSPRRPDVAALQSRIENREATVGIVGLGYVGLPLVLAFCRQGL
                     RTIGFDILADRCRQLLAGESYIRHIASAEIAEQVATGRLDATDDFARVAETDALLICV
                     PTPLTRHRDPDLSYITATGRAIAPHLTPGCLVCLESTTYPGTTDEELRPILEASGGKA
                     GIDFFLAYSPEREDPGTAEHSAARIPKVVGADDPASLALALALYSHVVPRVVPVRDTR
                     TAEAVKLTENIFRSVNIALVNELKVIFTRMGINVWDVIDAAKTKPFGYMPFYPGPGLG
                     GHCIPIDPFYLTWKAREYEVNTRFIELAGEINSQMPEYVVERLAHALNDQGKPLRGSR
                     ILLLGVAYKNDVDDDRESPSYRLFELLLKARAEVEYYDPYIPVIRPTREFPHLAGRES
                     IVWSPETLAGFDAALIATAHTPVDYAELVRHCPLVVDTRNATRDVTEGREKIVPA"
     gene            complement(12433..13215)
                     /locus_tag="PLS90_04090"
     CDS             complement(12433..13215)
                     /locus_tag="PLS90_04090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK01617.1"
                     /translation="MIRRLTESDRASTLDLLGAAPEANVFLVGDIHNHGFDAPFQQVW
                     GDFGPDGALRAVLLRYYRSLVFYGRGGFDFLGLAQLIESLDAGILSGEYDAVAPFERI
                     GIYQPARRLLLCRLSEPTAGASPRGAPEPRRLGPDDAPRIHELRERIGEFTQTVGVEE
                     VRAGLARGTDRSYGIELDGRLVSVATSTAENPFTAMIVGVCTHPAHRRRGLASRCMDR
                     LCRDLLRDGRTPCLFYDNPEAGGIYRRLGFQEVGAYGILERA"
     gene            complement(13339..14784)
                     /locus_tag="PLS90_04095"
     CDS             complement(13339..14784)
                     /locus_tag="PLS90_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01618.1"
                     /translation="MNPGRDSVLRATPARRWLVAGLLILACASAPAADPDAPAASGAV
                     PTREEIREKELQLLRRQLTELRRKYEENLISRLVYETQVQVAAIRGFQIEAPVQFKPL
                     TNRVLDQILMETVERSYPGPTLTLHVWLNELFGALPEGIDLLKLYRDLMGEQAAGLYD
                     PESKTLFVREDFPMEQAMGRLILAHEICHALQDQQFSLAEMGVEDTGNADRSLATLAL
                     AEGDATLLMGEHMARYGNPLSLFSNLPDMMLMNQEQLNRAPRAIQRELLFPYLDGMRF
                     FEELNGRLPADSEHAGQVADSPAEWRNWVWLDPPVSTEMILHPEKYLARELPVEIEPL
                     GEDDEHSAETTIGEFGMSLLLEAALGHERANEAAAGWHGDRIRLSQELDGEGQPAIRR
                     LHWVTHWDSPGDADQFAQALVDAFNERIDGLTWEEAGPERKADAPSAELTLTRPGETS
                     VELVGLFRLNGAGEEAAPTSFAAAGGSAGVK"
     gene            complement(14817..15998)
                     /locus_tag="PLS90_04100"
     CDS             complement(14817..15998)
                     /locus_tag="PLS90_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK01619.1"
                     /translation="MFLHLDCRHYRGDRPCLHHRPCEGCPHYAPMGPRVLIIKLGALG
                     DVVRTGCLLPALAGWPEPPHVTWVTAPAAQPLVERLTVAGRPAVHRVLPFTTETLAHL
                     ELEHFDCVLSLDKEPGPCALAMRIEVRERLGIGLSRYGTVYPLNEECEYYFRLGLDNE
                     EKFFRNRKSYPRLLFEALGWDYGGERYELTPTAADEAEADRRLRGAGLPAGAPFLAVN
                     PGAGGVFANKAWRTEGYMELIQRLRAERPELHFVLTGGPEDSARLDAIAAAVGPDRMH
                     APGADLPLGPFAALIGRAAVLVSGDTLAMHLAIAQRRRVVAIFGPTCPQEIELFALGE
                     RVVTSADCAPCYLRQCDKTTTCQDLISTAEVQAAVARQLEAAAAAAHAPVEQTGAREI
                     S"
     gene            16340..18145
                     /gene="lepA"
                     /locus_tag="PLS90_04105"
     CDS             16340..18145
                     /gene="lepA"
                     /locus_tag="PLS90_04105"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPK01620.1"
                     /translation="MNSNPLIRNFCIIAHIDHGKSTLADQLLLRTGTLSQREMREQVL
                     DTMDLERERGITIKAQAVRMMYRARDGQTYELNMIDTPGHVDFSYEVSRSLAGCEGAL
                     LLVDAAQGVEAQTIANAYLAIDSNLEIIPVINKVDLPNADIPGVKSQIEEALGLDTET
                     AILASAKTGAGVDEILEAIVARVPPPRPSGADTTRALIFDSAYDNFRGVVTYIRVFEG
                     SIGLGDKVRFMKTGRDYDVTEVGYFSPRMVSTDRLHEGEVGYIIAAIKDISSVKVGDT
                     ITQAQRPAATALAGYRESKPFVFAGLFPTNTEQFEDLAVAIEKINLNDASFIAERENS
                     EALGFGFRCGFLGMLHMEIIQERLEREHGLDLVLTCPNVGYRVTLSNGEVVHIDKPAD
                     LPDVTRIALTEEPYIEGRFLLPADYVGALMTLAQAKRGIQKGMQYLGTSRVMLTYELP
                     LSEIIFDFYDKLKSATRGYASFDYEIIGYRPVNMVKLDILINGDPVDALSTLVRREDA
                     QWRGRQLCEKLRQIVPRQQYEVAIQAAIGGKIIARETVKALRKNVTAKCYGGDITRKR
                     KLLEKQKAGKKRMRQIGRVEIPQEAFMAVLRTDEG"
     gene            complement(18449..19594)
                     /gene="mraY"
                     /locus_tag="PLS90_04110"
     CDS             complement(18449..19594)
                     /gene="mraY"
                     /locus_tag="PLS90_04110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPK01621.1"
                     /translation="MLILFLDLIERLLKEDYDMGPARVLTFITVRAALALALAFAISL
                     LIGPWVIRRLAALKAGQVIRHSRGDNAVDLAAMHGAKAGTPTMGGLLMLFSLLLPALL
                     LCRLNNIHFILLLTMTFGYGALGFWDDYLKIVRKHHGGVSPWRKIAVQAALAVVLAFT
                     LYAGDWGVYYHPADASGYPFLMLPFFKYLYLPLGLFFIPFVVLVLLATSNAVNLTDGL
                     DGLAIGISIANIAAFLIVAYLMSRADFSRYLYVPYIPGGGEMVVFLAALLGSSLGFLW
                     FNAPKAEVFMGDTGSMMLGGAIGTTAVLLKQEMLLLVIGGMFVIEELSVVLQVCCFKL
                     TGTRIFRMSPLHHHYEKLGMPESKIIIRFWLVSWILSMAGLAMLKLR"
     gene            complement(19644..21116)
                     /gene="murF"
                     /locus_tag="PLS90_04115"
     CDS             complement(19644..21116)
                     /gene="murF"
                     /locus_tag="PLS90_04115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPK01622.1"
                     /translation="MTPAGLPPLEALTVAEAASILGARYDPIHAERTFGAICTDSRAI
                     APGQYFLALRGETHDGHRFCGAAVERGAAGLIIARDFWPADPLPDHLPILRVEDTLFA
                     YGQLAADRRRRWGGRVLALSGSAGKTTTRRLLAAALARRFPTLEPLHNYNNLIGVPHT
                     LLRLNADHQVAVLELGMNQPGELARLTRIADPDAALLTQIGLTHVGMFPSVDGLIDAK
                     LDLFRNTAAGRPLVVNAGCPRTMMRMAEFSRDYPITYFLGEGPARYEVEPAVRISEVR
                     ALSPVHALPDVGYRFNLELPGGELRDLELRLFGRHHLENVAAAAALLLAGGYDPAWIA
                     DALPEFHTEPLRGQVVRAGELTLILDCYNASPPSMLGALQSLAHLPLMGRRVLVLADM
                     LELGAESEVAHEMLLGPLREIGPTPFFGLGTYCAHLAEMLFTEGWPASGFDEQSALVT
                     SLRETVRPGDVVLFKGSHGFALERVAQALVPDVPILPDGH"
     gene            complement(21236..21835)
                     /gene="lexA"
                     /locus_tag="PLS90_04120"
     CDS             complement(21236..21835)
                     /gene="lexA"
                     /locus_tag="PLS90_04120"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPK01623.1"
                     /translation="MYLTRRQREMLNVIEQFIEENRYSPTLEEIADQLGLSSLATVHK
                     HLQNLEQKGLIRRQWNHSRAIEVTSKARVRGNGLPLMGEVAAGLPIEAIENPEYIDVP
                     RELVGRRESFVLRVRGDSMIDDGIRDGDLLVVESRRSALPGQTVVALIDGAEATVKRF
                     YPEGEIVRLQPANETMSPLRYPAARVRIQGIVVGLMRKY"
     gene            complement(21987..23117)
                     /gene="mltG"
                     /locus_tag="PLS90_04125"
     CDS             complement(21987..23117)
                     /gene="mltG"
                     /locus_tag="PLS90_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPK01624.1"
                     /translation="MTPEPTQEPSPTTAPTPAAPRRRWLRWTAAALLLLALLTAGAAV
                     GGWEYLSWWGERPYGSAAERSVTIVAGTSARAIARRMHELGLVSRPELYLLWLKREGK
                     LASLHAGRYRLRGPISPRELTEQLRRGSFQRKLTIPEGWTARRIGERLASEDWITTGA
                     LWLSVLEQPGELPRAFIDATGIEPTSGTLAAAGGAPLLEGFLFPDTYLIEAGSPPRAI
                     LQAMLEEFARQWRAADPARRGAPAQDLTPRQIVTLASMIQREARSAGEMPLIASVYYN
                     RLARRMKLQCDATVHYALGDVWERKLTYADLEVDSPYNTYRHEGLPPGPIANPGRAAL
                     EAALRPAETDYLYYVYAGGDQHIFSATWREHQRAVRAARRRE"
     gene            complement(23114..24313)
                     /locus_tag="PLS90_04130"
     CDS             complement(23114..24313)
                     /locus_tag="PLS90_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01625.1"
                     /translation="MLNHNLRGCGTWHRAWRLARELAGRGHAVTLWTAAPRHFYRPQR
                     MTEAGVRIVETPSWAPLAGADDGWGPLDAAWRAGRALLDPHDLVYAFAHPPNVALPAF
                     VAHRLRGRPLLADWCDWYAGGVFPKRERMRREGLIPPGEKFLQARAERWELALERRVM
                     RAAGRLTVISRRLREVALALGRREAEILLLPNGANLDGIRPQERGACRRELNLPADAP
                     LLGYIANYHPDQELMVRTLMRARERLPELRLVVAGPPLAPALIRDLHAREAVIELGRL
                     PVDRMQIVLGAADALLLPLEDNEHNRARMPFKFTDYLAAGRPVVTSPVGDLADYFGDA
                     AAPIGAAAPPEPDALAAAIAELFHDPARIEAMGHAARRLAEREFSWPRLAEQLERFID
                     QWLAKEK"
     gene            complement(24388..25011)
                     /locus_tag="PLS90_04135"
     CDS             complement(24388..25011)
                     /locus_tag="PLS90_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HPK01626.1"
                     /translation="MFELTQWQDWARLAMIPVVGGLIGWATNWLAVKMIFRPRRPRRI
                     LGFTLQGLVPKRQRELAVSIGQTVERHLISHDDIESILLGQRTQDAIDGLVRDKFNRF
                     VDRRLRRLNPLLGVMLRGELRRKVERLLVAEARRLAPRAIGQVIDRLEDELDFGALVE
                     EKVRQFDVVRLETIIFEIARRELKAIEVLGGVLGFLIGLLQMAILLI"
BASE COUNT         4243 a         8449 c         8355 g         4084 t
ORIGIN      
        1 tcattctcat tggattcatc atgatgctcg tcaatcagtt atgaaaccag ttctattcaa
       61 cctgataggc ctccagggcg gtggccaggg tgcggaagtc ggcgtgaacc cgggtgacct
      121 tggcgcggac ctgggcctcg aggaagttgg ggacggcgat cgcggccaga atcgcgatga
      181 tcgccacgac gatcagcagc tcgatcagcg taaagccggt gcgcgagggt tgcgtcatga
      241 tggcagctcc ttacggtccc cagcggacaa ggtcgccgct gctgaccgtc ccgttcgtcg
      301 ggtcgtagat catttcgggg tagcgccgga tcacggtcgt cccgttggtg gcgacggtca
      361 taaacggcac ctcgccggcg aggagagcgg tttcgccgaa atcgcagaac aggtccgggc
      421 cgtgggagct ggcgatccat ttgccgcgga agccgtggat cgcccgggcc ttgacgaccg
      481 gatgattccg ccagccctcg ccctgatacc agtagcgcgg cggctcggtc ggccacatct
      541 cgacgtcgaa gaacttgtcg gtcgggatcg aggacaggta ggcgaccggc gtcgtcagga
      601 agttgaacgc gtagccgttg ggcacgggca cgccggccag tgtcatctca tcacgatcgg
      661 ggggataatg ggcgttatca acctgatagg cctccagggc ggtggccagg gtgcggaagt
      721 cggcgtgaac ccgggtgacc ttggcgcgga cctgggcctc gaggaagttg gggacggcga
      781 tcgcggccag aatcgcgatg atcgccacga cgatcagcag ctcgatcagc gtaaagccgg
      841 tgcgcggtgg tcgcctcagc atatgacggc tccttttaga agacacttgc atcctatttg
      901 atgtcaaagc cgggtcgggt ggccaagccg gcggtttcga tcaaccaatc tgagtatcct
      961 tttgtgcgcg cgaactgggg ggacgggtcc tgctatggtg ggcagtatgg ttcatactac
     1021 gcgtgtagtc aagttttttt tcgcggccgc tcgcgggaga tcggagagcg acctccagat
     1081 ggtgaccatt cgatcatgaa taattgaaaa taatgcattt ataatgatct tggaacgtgc
     1141 tcggggagac ggcgaacgca ggaatcctgg ataaagcctt gacgcggcgt ccaaaaagcg
     1201 cgtattgtca ggactcgttc agctcgtgga gcggatcttc ggacacatga ctcctgatca
     1261 ctgcaacggc ccaattcggc aagcccgggg cgccccgcgc tgctggctcc ggttgccgcc
     1321 gcgccaggcc catcgtcccc ggtgggcttt gacgggaggc ccccggtgag ccgcacgaac
     1381 ctgaccatgg ccgatattgc gcgcatttgc ggcgtcgccc gctcgacggt ccacgcggtg
     1441 ctcaagggca agccgggcgt ctcggcccgg acgcgggcga aagtcctgga tgcggtcgcg
     1501 cggttcgatt actcaccctg cccgattgcc aagagcctcg cccgcaagaa gtccggcctc
     1561 gtcggcgtcg tcatccgcga cctcgccaat ccgttctacg atcagttcct caagcacatg
     1621 gaccatgtcc tgaaggatct gcgggtcatc acgttcaaca cgcgccacga catcgacaag
     1681 gagatccacg cgctgcggac gctggcgggc tatcgcgccg acggactgat cattgctccg
     1741 gtgcaaccgg agcgcgcggc cgatcatctt ggcgactggg tcgagcgcgg cggcgcgctg
     1801 gtctcgctgg accggctgcc ggggatcgaa tgcagttatg tcgagttcag gaatttcgac
     1861 atcggccgga tgacgaccga atacgccatc cgcctgggcc accgcgccat ctgcatgctg
     1921 gccggcccgc cgcgcgacga tgcccgggac gacgagcgcg tggccgggtt caaggcctgc
     1981 ctgcgcgagc agggcctcga tccggccgag tgcacggtgc agcatgtcgg ctggcgctgg
     2041 gagggtggat tccacgccgc cagcgaattt ttcagcggcg ctcgccgctg tccctcgctc
     2101 ttcatctgca ataatgacta cgtcgccggc ggggtctaca aagcggccta tgtgcatcgc
     2161 ctgcgcatcc ccgatcaggt cagcgtgatc ggctgcgacg atatcggcct ggccgccgtg
     2221 ctcggcccgt cgttgacgac ggtgcgcctg aacgcgcgcg gcatcggcga gggggtcgcg
     2281 cggatgctgc tgcgccagat gaatcagggc agcgcgttcc agccgcgttc gatcgccgtc
     2341 gcgccgatgc tcgtcgagcg cgagtcggtg ctgaacctga cggccaccga ttcggccgaa
     2401 gcgtgctgcc aggcatgccg cgagcgggcc gacgcggcgc agcgggagga agcggcatgc
     2461 ctgagcaagg gatgaatgtc ggtcggcctc cggggacgtt catgagttgc ttttgaacct
     2521 atgggttaca ataaaggact aagtttagga ggtattcaat agccagggac gagcgccaaa
     2581 ttgtggggcg gggcgttcgg gccgatcacg agctgctcgg gaccgagggt tcacaacgag
     2641 gggacaagat ggggcaggcc ctcgggagcg aacacgagga cgaggaagag ggacgaattg
     2701 ggagagataa caagcggggg ccttagctga ccccttccaa cggccgcggc cttgacgaat
     2761 ccacggcccg tcccgtcagg gacggcagac ttcagcccgc tgcttcggtg gcgggaaatc
     2821 agggacggta gaactgctcg aaacgaggac gagcgacgag aaacgtattt agacgatttc
     2881 cagcacaggg agttcatcat gagacacagc aagccgcgga tgcgcgcgag ccaccggatc
     2941 gggtgcgcgc tgctcattct gttcgccgcc gcgacggcgc gcgccgggca accggtcctg
     3001 ttcagcgagg actttgaagg ccgccccgcc ggggaggcat tgcacgggac gccgctcaac
     3061 aacgggctgg gcggccaggt cgcggcgacc tggtgggacc ccaagggact cgcccatgcg
     3121 ggagcggatg aacagggggg ccgcggcgag attgccccgg gcggctggga tcacttcaag
     3181 accctcatcg actatgagct cgatccggac gaggcctacg ccttcgaact ggagctgcgc
     3241 ccgcccggcg gcgttttgca gccgactccc tggaaccccc actcggggct ggtgattctc
     3301 tcgcgcaagg atacgggcat gatggccagc ggcgtcgtgc tgcgcatcca gggacccgat
     3361 ccgagcggca aggaccccaa tcgcttcacg ctggtgtatc agcgcgagga gggaaattac
     3421 ttcgacgacc cggcgctgat cggcgacatc gttccggagg attacaacgc cgcgggcgtc
     3481 atcccgacgc gaattgaact cagcggccgg ggaacggcgg cgcacccgct ggtggggcgt
     3541 ctgctgctga acggccgtga acgcggctcg ttcgtgatcc ccgatctctt ccggggcggg
     3601 cgctacatcg ggctggtgac gcagtcgagc ccgaaagagg gcgccgcgcc cggcctggtg
     3661 gacaacgtga agctggagcg gctcgaagcg cccgtgttca acccggcgcc gcgggtggcg
     3721 atcgcgccgg tgctgctgcc ggtcgcgatg ccggagctgg aactgacggc gtttgtccgc
     3781 gacgacggtc cgaccgaccg gttgcgcctg ggctgggaga tgattgacgg accggccgcg
     3841 gcaactttct ccgacagcgg ccggacgacg acgcgagtta cctttgcggc gcccgggctt
     3901 tacacgctgg ggttctccgc gttcgatggc gaacaccgcg ccgaagagcg actctcgctg
     3961 gtcgtggctc cgccgccgag cgaggcgccc ctggccgaag ccggcttctc tgaaggggag
     4021 cccctcgccg cgaaagacgc cctgccgggg caagtgctga cccgtccgct gccgctcgcg
     4081 gcggacgccg gccatgggct gcgggcgcgc tggcgcatgg cgcctccctc ggccgccggc
     4141 tggggtgggt cggaccgcat tatcgtcgcg ttgacggatg ccgaagcgac cgtcggccgc
     4201 gaaattcatc tggtcccggg cgacgccgag gcggccggcc ccgctgctgc cgaaccggcg
     4261 ggcgatcggc cttcgccgat cacgctgccg atcgccgccg ctgcgcggga tgaactcctg
     4321 ctgcagctcg atctggccgg aacgggcacg ccgcgcgatc cgctgcgcgg ccggctgctg
     4381 gtcaatcagg aacgaaagct ggatttcgat gcgggcccgc tggcggcgga tgtccgcttg
     4441 agcgtggccg tcgtgaccga gggcggtgcg ccggtgcagc tcgacgattt cgaactggcc
     4501 gccctgcgta atccctttgt cgtggaggat ttcatgctgc tctacagcac gctcggctac
     4561 gacagccgcg cctccaagcg cgcgctggtg cggttgcata cccgttccga cgccgaatcg
     4621 ctcgacgcgg aagcaagctc ctggcggttg ctcaatcagg aaggcgagcc cgtgctcagc
     4681 ggacccctga tgccgctgcc caagaccttc ggctgcctgc tgtgggaggc cgaattcagt
     4741 tcgtggacca cgcccgggac atactcgctg gaggtgacgc tggtcgaccg cgacgcggga
     4801 gagctggcgc cgctcgtgtc cgaacccttc ctgatcgagc cgcggctgca ttaccagcgg
     4861 acgttcgagg ggatcgcgct gttgaacgcc gaggcgcggc gcgcgacgga ggatgtcggc
     4921 ggcggctatt atgactgcaa ctcggcgatg ggcgaggcgt attcgcacgg ggcgttcctc
     4981 gcggggctgt ccaaagcgta tcagcgtttc gccgatgatc tgacgctgac ggaccgcgat
     5041 cgtctgcgcg aggcggccaa tcgcgccgcc gattacctga tgctgctgca caacgcccag
     5101 acgggcgaga tcatctgcca gcatcccaac cgcgtgcagg gggcggagag cggcgacggt
     5161 ccgcacaaca cgttcgaggg gatctggggc ctgctcaatt acgccgtggt gttcaaggat
     5221 ctggagccgg cgcgcgcgct cgacgccttc aatcgctcgc gcaaatcgta tcagtggctg
     5281 ggcaatcagg ggatctacta tcccgaccag gccagcgcgt tcatggcgct gatccatcaa
     5341 taccagccga cctacccggt ccaggaaaca ctgttcagga cgctcaacga ctttctgagg
     5401 gaattcaacc cgcgccagtg ggggcggagt tgcggccgct gcatccccta cttcgagggc
     5461 ctgtggtact gcctgcgaac cttcccggac catcccgacc ggtcgcggtg ggagtcgctc
     5521 gcccgcgatt tcaaccagca gtggtatcag gagatgatgc agcgcaacgc gtttcacatc
     5581 ctgccgcagg gaggggcgcg cgagtgggac aacaccgagg atgtgccgcc ggaaatctac
     5641 ccgccctaca acttctacgc ctccacgcag ttcggcacga tggcgaccga cgcgatcctg
     5701 ctggcgcgga tcaccggcga cgattcgctc gaacggatgg cgaccggctg cctgaactgg
     5761 atcaccggcc tgcacccggg cgtgccgcgc aacagcacgc tgccggcggc gaccggccgg
     5821 cggttgaccg ccgcggccct catcatgaat ctccccggcg cgcgccacat taggacctgg
     5881 acctactggg gctgggagca gcccgagatc gagtacctct cgatcgtcaa cgggctgtgc
     5941 atcgacgacg gggactggta ctacagcacg cgcgaatggc gcagcggcga gacgttcatc
     6001 aagcacgacg gcgcctacct ttacgccatg gcgctttacg accaatatct ggaagaaaac
     6061 cgctccggcg tcaccagcct ggcgcaactg gcgcaatatg aatgacggcc ggtccggcgc
     6121 ggcgcgcggc gggggcaccc accatggccc ggaagcacta ccggaacgag atcgagggtt
     6181 acctcttcct ggcgccgcag ttgctggggc tggtgctgtg gctcggcatc ccgatgctgg
     6241 cggccttcgc gctgagcttc acccactggg acctgttcgg ccggcccgag ttcgtcgggc
     6301 tgcgtaacta catcaacctg ctctttcacg accgccggat gtgggcgacc ctgttcaaca
     6361 ccgcctactt cacggcgggg accgtgacgc tggggacgac gctggcgctg ctgctggccg
     6421 ccgccgtgca ccaggtgtcg ttcggccaga cgctgttcaa gtcgatctat ttcctgccgg
     6481 tgatctgctc gatgacggcg attgcggcgc tgtggcgctg gctgttcagc ggcgatttcg
     6541 gcctgctcaa tctctatctg gagcgcatcg gcatctcggc ccccgactgg ctcaacgata
     6601 cccgcttcgc gatgcccgcc gtcattctga tgagcgtctg gaaaggggtc ggctttccga
     6661 tgatcatctt tctggccggg ctgcacgaca tcccggccgt ctactacgag gcggcggaga
     6721 tcgacggcgc cgatcgccgc cgccagttcc tccacgtcac gctgccgcag ctctccccga
     6781 tcatcctgtt cgtcgtcgtc acctccgtca tcggctgctt tcagaccttc gatcagatct
     6841 acatgatgac ctcgggcggc ccgatgggca gcacgcggac gatcgcgttc tatctgttcg
     6901 aaaatgcctt tgtcttctac aagttcggca acgcctgcgc catcgcctac gttctgttcg
     6961 tcctgcttgg cggcttcacg catttctcga tccggctgac gcagcgctgg gtgcattacc
     7021 aataggaact cttgatttcg aaaaacacaa aacggcacga aggggcttaa cggcggagaa
     7081 caggaacgac gggctcggaa ggttgagcag acagaacccg acttgattcg ggtccctcat
     7141 cgcccttcca tccatgacgt ccgttctgtc gcttcgctca gaccaggaag gaatcgcatg
     7201 cggcgcggat cgcataaact cagccggcgg ctgtttctgc tcctgctcta cgccgtgctc
     7261 acggccggcg ccgtcgtcat ggtcgcgccg ttcctctgga tggtctcgac ctcgctcaag
     7321 gcgccgcacg agaccttcat gcttccgccg atctggattc cggaggactt ccggttcgag
     7381 aattacgccg aagtcttcga ccggctcccg ttcttccggt ttctgctcaa cacggcgacg
     7441 atcgccgggc tcgtcacgtt cggcgtcctc ctctcgtgtt cactgaccgc cttcgccctc
     7501 agccggctgc gcttccgcgg ccgcgacctg gtcttctata ttctgctggc cacgatgatc
     7561 attccggcgc cggtcacgct tgtgccgatt ttcgcgatca tgcgccggct cgaatggacc
     7621 aataccttcc tgccgctcgt ggtcccggcg ttcttcggcg gcgcgtttcc ggtcttcctg
     7681 ctccgccagt tcttcatgcg cattccccgc gacctcgacg aagcggcggt gatggacggc
     7741 tgcagcgcct tcggcatcta ctggcgggtg attctcccgc tcggcaaacc ggctctcgcc
     7801 acgatcgggc tgatcagctt catgggggcc tggaatgatt tcttcggccc cctgatctat
     7861 ttgtattcga tcaagaaaca gacccttacg ctcggcctgg ccacgtttca gggttactac
     7921 gccaccgact ggcacctgct gatggccgcg acggtgatcg tcacgctccc cattctgctg
     7981 gcgtttctgt tctcccagcg gttcttcgtc gaggggatca cgatgacggg catcaaggga
     8041 tagcgcccgg gggatgaacg acgccatgaa ccatcgggac cctcggcgac aatcgcctca
     8101 tcgacccgcc ggccgctgtt tggccggggt cgtcgcgcga ctcctcgccg gagtcgccgt
     8161 tctgcttgtg gcggcctgcg ggggcgaaga cgaagaccgg cggatcgaac tgcgctgggg
     8221 cctgtgggga acgcccgaag cggtccagaa tcagcgccgc ttcgcccggg atttcatgcg
     8281 actgcatccc aacgtgcgcg tcgagatcga gccggtcgcc ggcgacgtca ccgtcgcctt
     8341 ctcgatgcgc ctggccggcg gcgacgcccc cgacgtcctc tatgccggca actggttcct
     8401 gtttgaattc gcggacaagg gcacgctgct gccgctggac gacctgatcg cgcgcgatgg
     8461 catcgacatg gggcagttct tcgattcggc gctcagcggc atccagtggg acggcgcgac
     8521 ctacgccctg cccgaggcca tcaacgtcga gatgctcttc tacaatcagg atatcttcga
     8581 tcgctacggg gtgccctatc ccgaacccgg ctggacctgg gacgactatc tggagaaagc
     8641 ccggcgcctg acacaggata ccgacggcga cggccgcacc gacatctggg gctgttacgt
     8701 ctggacctgg atccccggct ttctgccgtt catgctgcag aacgagagca acatgttcga
     8761 cgacgatttc cgccggttca cgatggacga cccgcgcgcc atcgaagcgc tgcaattcat
     8821 tcaggatctg atcttccgct accgcgtggc gccggggcgc caggcgctgc aatcgcaact
     8881 gagcatcgac atgttcgcct cggggcggat cgcgatggga agtttcggtt actggggcgt
     8941 gcgcgaactt cagggcgcgc cgttccgctg ggatgtggcg gagcttcccc gccggcgcac
     9001 gaccggcaac gtcgtccatg tgcacagctt cgcgatcaat cgcgagaccg agcatcccga
     9061 ggaagcgtgg gaactgatca aatacctgac gctggacgag cccgcgcgcg aggtcgttta
     9121 cctgatcccg gcccagaagc ggatcgccgc ggagatgatc cgcgccggga acgaggggat
     9181 gccggccaat gcggaggcga tcctggcgac ggccaccgat cccaacacgg tctcgccgcc
     9241 gttcggcccg cgcttcggcg aatacagctc ggtcctcggc ctggaactgg acggagtgtg
     9301 gctgggcaaa gtctcggtgg aagaggccgt gcgcaaggcg aagccgcaga ttgagctgat
     9361 cctcagcgaa tgaccggccg ggacgcggcg gagccggaga ccgacgcatg cgccgacggc
     9421 cggaccgctc aaccgagaaa aaatcgtctc acgcggagac gcggaggaag aacccgcatc
     9481 cttcttgcat gacttcgtgg gaacttcgtt gaacccgcgc cgctcgcacc gagttcaata
     9541 accgcgggcg cgggcctgac gcggcgcgat ccggctgagc gacttgaccg actcgcgctc
     9601 caccagcacc ggcgacaccg ggatcgacac gcacttgaaa tcctcgccgc cgcggatctg
     9661 ctccagcagc gtcaatgcca ccttctcgcc gatatcgcgc acattcagat tgatggtcgt
     9721 caacgacggc cccagcaccg aggccaggtc gatatcgtcg cagccgatca cgctcaggtc
     9781 gtccgggatc cgcacaccgt ggccgtaagc ggccttgtag accccgccgg cgacataatc
     9841 gttgccgcag atgatcgccg tgaacgcgtc gcggttgtgc gcgagcagct tgctcgccgc
     9901 gcggtagccg ttctcccagc ccaggccgac ccggatcgtg tcgcagtcat acggatcgat
     9961 gccgtaatcg agcatgcaca tcttgaagcc gacgacccgc tcatcgtcgt ggtgctcgcg
    10021 ctggcgcgga ccggcaaaga agcagacgcg ccggtggccg tgactgaaca ggtgctcggc
    10081 cgccatatag ccgatgtgcc ggtcgttcag atcgacgaag ttggagtcgg tgccggtcag
    10141 gcgatccatc gagacgaggg cgcgcccctg gcggacccag ttggtcaggt gggtcgtccc
    10201 cagttcggga cgggcggggg cgatgatcag cccgtcggcg cggtagccca tcagcgtctc
    10261 caccgcccgc gcctcgcgcc gcggatcgtg gcgcgtgttg aagtagacga tgacgtaatc
    10321 cttgagcaaa tcctcgaccc gctccaggaa ctgatcatag aacgggttgg tcaggtcgcg
    10381 gatgacgacc cccaccaggc cggaaaggcc gctggccagc gtcttggcga tcgggcaggg
    10441 cgaataattc agcttttgga cgacgtccat caccttattg cgcgtcttct cggtcaccca
    10501 cggcttgttc gtgaagacgg catggacggt cgagcgggcg acaccgcaga tcttcgcgat
    10561 gtcctccatc gtcagctttt tctcgttcgt cgccattggc ccgcacctgg tcgggtatcg
    10621 tcgatcatag cacaaaaaga tgcgcgtagt caaaatcgca ttccgccagc gccggaaccc
    10681 gcgcggagcg gccggttggc gcgcaaccta ttggcgaact ttacgttagc tcgctcccgg
    10741 cgtggattcg ggagcgcccc cggcgccgcc cgccgacccg gtcccgcgcg ccgcggacta
    10801 cgcgcgtcgc atggcccgcc gctcggcgac ccgcccagcg accgcccggt tgtcagcggc
    10861 tcacgccggt acgatcttct cccggccctc ggttacgtcg cgcgtggcgt tgcgcgtgtc
    10921 gaccaccagc gggcaatgcc tcaccagctc ggcgtaatcg accggcgtat gggcggtggc
    10981 aatcagcgcg gcatcgaatc cggccagcgt ctcgggcgac cagacgatgc tttcgcggcc
    11041 ggccaggtgc ggaaactcgc gcgtcggccg gatgaccgga atgtaggggt cgtaatactc
    11101 aacctcggcc ctggccttca gcagcagctc gaacagccgg tagctcgggc tttcccggtc
    11161 gtcgtccacg tcgttcttgt aggccacgcc cagcagcagg atccgcgagc cgcgcagcgg
    11221 cttgccctga tcattcagcg cgtgcgccag ccgctcgacc acgtattccg gcatctgcga
    11281 gttgatctcg ccggccagct cgatgaaccg cgtgttgacc tcgtattcgc gcgccttcca
    11341 ggtcagatag aacgggtcga tcgggatgca gtgaccgccc agaccgggcc cgggatagaa
    11401 cggcatgtag ccgaacggct tggtcttggc ggcatcgatc acgtcccaga cgttgatccc
    11461 catgcgcgtg aagatcactt tcagctcgtt gaccagggcg atgttgaccg agcggaagat
    11521 gttctcggtc aacttgacgg cctcggccgt gcgcgtgtcg cggacgggga cgacgcgcgg
    11581 cacgacatgc gagtacaggg ccagggccag ggccaggctg gccgggtcgt cggcgccgac
    11641 gaccttcggg atgcgcgcgg cggagtgctc cgccgtgccg gggtcctcgc gctcgggtga
    11701 ataggcgagg aagaaatcga tccccgcctt cccgccgctc gcctccagta tcggacgcag
    11761 ttcctcgtcg gtcgtgccgg ggtaggtggt cgattccagg cagacgaggc agccaggcgt
    11821 caggtgcggc gcgatcgccc ggccggtggc cgtgatgtaa gagaggtcgg gatcgcgatg
    11881 gcgcgtcagc ggcgtcggca cgcagatcag cagcgcgtcg gtctcggcca cgcgcgcgaa
    11941 atcgtcggtg gcgtccagcc gtccggtggc gacctgttcg gcgatttccg ccgacgcgat
    12001 gtgacggatg taggactcgc ccgccaagag ctgccggcag cggtcggcca ggatgtcgaa
    12061 gccgatcgtc cgcaatccct ggcggcagaa cgccagcacc agcggcaggc cgacgtaacc
    12121 caggccgacg atcccgacgg tggcttcgcg attctcgatg cgcgactgaa gggcggcgac
    12181 gtccgggcgg cggggggagc tcatgcggtt ctccggggct gcgggctgcg gtgcggccgg
    12241 cgcgagcggc ctggcgcgcg gacgcgtcgc cttccccgtc ggccggcggt ggtttcggca
    12301 aatcttcggg aaaacagccc gaagtggcaa tcacaaaggg gcgattgcgc gcgctggggc
    12361 gccgcgatgc cgccggtgtg gggcccgcgc cccgccggtt ttgccccgcc ccgtcccggc
    12421 gcccgcgccc gatcaggcgc gttccaggat gccgtaggcg ccgacctcct ggaacccgag
    12481 ccgccggtaa atgccgcccg cctcggggtt atcgtagaac aggcagggcg tgcggccatc
    12541 gcgcagcaga tcgcggcaga ggcggtccat gcagcggctg gccaggccgc ggcggcggtg
    12601 cgccggatgc gtgcagacgc cgacgatcat cgccgtgaac ggattctcgg ccgtcgatgt
    12661 ggcgaccgac acgagccgcc cgtcgagctc gatgccgtag ctgcgatcgg tcccccgcgc
    12721 caggcccgcg cgcacctcct cgacgccgac ggtctgggtg aactcgccga tccgctcgcg
    12781 cagctcgtga atgcgcgggg catcgtccgg tcccagccgg cgcggttcgg gcgcgccgcg
    12841 cgggctcgcg ccggccgtcg gctcactcag gcggcagagg agcagccgcc gcgccggttg
    12901 gtaaatgccg atccgctcga acggggcgac cgcatcgtat tcgccggaga ggatgccggc
    12961 gtcgagcgat tcgatgagct gcgcgagacc aaggaaatca aagccgccgc ggccgtagaa
    13021 gaccagactg cgataataac gcagcaggac ggcgcgcaag gccccatcgg gaccgaaatc
    13081 gccccacacc tgctgaaacg gggcgtcgaa gccgtggttg tggatgtcgc cgacgaggaa
    13141 aacgttggcc tcgggcgcgg cgccgagcaa gtccagcgtc gacgcccggt ccgattctgt
    13201 caggcggcgg atcatctccg gattgtgcca gccggggcgg gcgaatgcga tccccgatcc
    13261 ggttcggaat ccgtcggatc ggtcagatcg gtcggatcgg tcagatcggt cggatcggtc
    13321 agatctgacg gattgcgttc atttcacgcc cgccgatccg ccggcggcgg cgaacgacgt
    13381 cggcgcggcc tcctcgcctg cgccattcag ccggaacagg ccgaccagtt cgaccgaggt
    13441 ttcgcccggc cgcgtcagcg tcagttcggc cgacggcgcg tcggccttcc gctcggggcc
    13501 cgcctcctcc cacgtcaggc cgtcgatccg ttcattaaag gcatcgacca gcgcctgggc
    13561 gaactgatcg gcgtcgcccg gcgagtccca gtgcgtcacc cagtgcagcc ggcggatcgc
    13621 cggttggccc tccccgtcga gctcctgact caggcggatc cggtcgccgt gccacccggc
    13681 ggccgcctcg ttcgcccgtt catggccgag cgcggcctcc agcagcaggc tcatgccgaa
    13741 ttcgccgatg gtcgtctcgg cggagtgctc gtcgtcttcg cccagcggct caatctcgac
    13801 cggcagctcg cgcgccagat acttttccgg atgcaggatc atctcggtcg agaccggcgg
    13861 gtcgagccag acccagttgc gccattcggc gggcgagtcg gcgacctggc cggcgtgctc
    13921 ggagtccgcc ggcagtcggc cgttcagctc ctcaaagaac cgcatgccgt ccagataagg
    13981 gaagagcagc tcgcgctgga tcgcgcgcgg cgcccggttg agctgctcct gattcatcag
    14041 catcatgtcc ggcaggttcg aaaagaggct cagcggattc ccgtagcgcg ccatgtgctc
    14101 gcccatcagc agcgtggcgt ccccttcggc cagcgcgagc gtcgccagcg agcggtcggc
    14161 gttgccggtg tcctcgacgc ccatctcggc caggctgaac tgctgatcct ggagcgcgtg
    14221 gcagatctcg tgcgccagga tcagccggcc catcgcctgc tccatcggga agtcctcgcg
    14281 cacgaagagc gtcttgcttt ccggatcgta gagcccggca gcctgctcac ccatcaagtc
    14341 gcgatacagc ttgagcaggt cgatgccttc gggcagcgcg ccgaacagtt cgttgagcca
    14401 cacgtgcagc gtgagcgtcg ggccgggata ggagcgctcg accgtttcca tcaggatctg
    14461 gtcgaggacg cggttggtga gcggcttgaa ctggaccggc gcctcgatct ggaagccgcg
    14521 gatcgcggcg acctgaacct gcgtttcgta gaccaggcgc gagatcagat tctcttcata
    14581 tttccggcgc agttcggtca gttgccgccg caggagctgc agttcctttt cgcggatctc
    14641 ctcgcgggtc gggacggcgc cgctcgccgc cggggcatcg gggtcggccg ccggcgccga
    14701 cgcgcacgcc aggatcaaca ggcccgcgac aagccagcgg cgggccggcg ttgcgcgtag
    14761 aacagaatca cggccggggt tcatcaggtt gagctgctcc agggtgggat ggcgggtcag
    14821 gaaatttcgc gggcgccggt ctgttcaacg ggcgcatggg cggcggccgc cgccgcctcg
    14881 agctgacgcg cgacggcggc ctgcacttcg gcggtggata tgaggtcctg gcaggtggtg
    14941 gtcttgtcgc actggcgcag gtagcacggg gcgcagtcgg cggacgtgac cacccgttcg
    15001 ccgagcgcga acagctcgat ttcctgcggg caggtggggc cgaagatcgc cacaacgcgc
    15061 cgccgctgcg cgatcgccag atgcatggcg agcgtgtcgc ccgagacgag gacggcggcc
    15121 cggccgatga gcgcggcgaa cggcccgagc ggcagatcgg cgccgggcgc atgcatgcga
    15181 tcgggcccga cggcggccgc gatggcgtcc agccgcgcgc tgtcctccgg accgcccgtc
    15241 agcacgaaat gcagctcggg ccgctccgcc cgcagccgct ggatcagctc catgtagccc
    15301 tcggtgcgcc aggccttgtt ggcgaagacg ccccccgccc ccggattgac cgccaggaac
    15361 ggggcgcccg ccggcagccc ggcgccgcgc aggcggcggt cggcctcggc ctcgtcggcg
    15421 gcggtcggcg tcagctcgta gcgctcgccc ccgtagtccc agcccagcgc ctcgaacaac
    15481 aggcgcggat acgatttccg gttgcggaaa aacttctcct cattgtccag cccgagccgg
    15541 aagtagtact cgcactcctc gttgagcgga tagaccgtcc cgtagcgcga gagcccgatg
    15601 ccgagccgtt cgcgcacctc gatgcgcatc gccagggcgc aggggcccgg ttccttgtcc
    15661 agcgagagca cgcaatcgaa atgctccagc tccaggtgcg cgagggtttc ggtcgtgaac
    15721 ggcagaacgc ggtgaacggc gggccgcccc gccaccgtca gccgctcgac cagcggctgc
    15781 gccgcgggcg cggtgaccca ggtcacgtgg ggcggttcgg gccagccggc cagcgccggc
    15841 agcaggcagc cggtccgcac gacatcgccc agcgccccca gcttgatgat cagcacgcgc
    15901 gggcccatcg gcgcgtaatg cgggcagccc tcgcacgggc ggtggtggag gcagggccgg
    15961 tcgccccggt agtgtcggca gtccagatgc aggaacaaaa agcttcgctc ctcccgagcg
    16021 gttaggatca gtctagcata gattcagcgt ccaaggttca aggttcaaag ttcaagattc
    16081 cttccgcggg ttccatcttg cgcctcttct tcgagctcct ttgcgacggt ccaggatacc
    16141 tcaaggcaag ccgctacttc cctttggcgc ttgaatcagt cccccgctcc acggtaaaag
    16201 agtaggtttg cggaaccgcg caccattcgc ggcccgtcca cttcctcatg gaacagcccc
    16261 aatcgctcac gatgggcacg acgccgctcc gatccctctt acagatgaat cttgaacttt
    16321 gacccctgaa ttttgaacct tgaattccaa ccccctcatc cgcaacttct gcatcatcgc
    16381 gcacatcgac cacggcaagt cgacgctggc cgaccagctg ctgctgcgca ccggcacgct
    16441 ctcgcagcgt gaaatgcgcg agcaggtcct cgatacgatg gacctggagc gcgagcgcgg
    16501 catcacgatc aaggcccagg ccgtccgcat gatgtatcgc gcgcgcgacg gccagactta
    16561 cgagctgaac atgatcgaca cgccgggcca tgtcgatttc agctacgagg tgagccgctc
    16621 gctggccggc tgcgaggggg ccctgctgct ggtcgatgcc gcgcagggcg tggaagccca
    16681 gacgatcgcc aacgcctatc tggccatcga cagcaacctg gaaatcattc cggtgatcaa
    16741 caaggtcgat ctgcccaacg ccgacatccc gggcgtcaaa agccagatcg aggaggcgct
    16801 cggcctcgat accgagacgg cgatcctggc cagcgccaag accggcgccg gcgtcgacga
    16861 gatcctcgag gcgatcgtcg cgcgcgtgcc gccgcccagg ccctccggcg ccgacaccac
    16921 gcgcgcgctg attttcgact ccgcctacga caacttccgc ggcgtcgtca cctacatccg
    16981 cgtcttcgag gggtcgatcg gcctgggcga caaggtgcgc ttcatgaaaa ccggccgcga
    17041 ttacgacgtg accgaggtcg gctacttctc gccgcggatg gtctcgaccg atcgcctgca
    17101 cgagggcgag gtcggttaca tcatcgcggc gatcaaggac atctccagcg tcaaggtcgg
    17161 cgacacgatc acgcaggcgc agcgccccgc cgccacggcg ctggccggct atcgcgagag
    17221 caagccgttc gtcttcgccg ggctgtttcc gaccaacacc gaacagttcg aggatctggc
    17281 cgtcgcgatc gagaagatca acctgaacga cgcgtcgttc atcgccgaac gcgagaactc
    17341 cgaggcgctc ggcttcggct tccgctgcgg ctttctcggc atgctccaca tggagatcat
    17401 ccaggaacgg ctggagcgcg aacacggcct ggacctggtg ctgacctgcc ccaacgtcgg
    17461 ctaccgggtc acgctgagca acggcgaggt cgtgcatatc gacaagccgg ccgacctgcc
    17521 cgacgtgacg cgcatcgcgc tgaccgagga gccgtatatc gaggggcgct tcctgctgcc
    17581 ggccgattac gtcggtgcgc tgatgacgct ggcgcaggcc aagcgcggca tccagaaggg
    17641 gatgcagtac ctcggcacgt cgcgcgtgat gctgacctac gagctgccgc tgagcgagat
    17701 cattttcgat ttctacgaca agctgaagtc ggcgacgcgc ggctatgcct cgttcgatta
    17761 cgagatcatc ggctaccggc cggtcaacat ggtcaagctt gacatcctga tcaacggcga
    17821 cccggtggac gcgctctcga cgctggtgcg ccgcgaggac gcgcagtggc gcgggcggca
    17881 actgtgcgag aaactgcgcc agatcgtgcc gcgccagcaa tacgaggtgg cgatccaggc
    17941 ggccatcggc ggcaagatca tcgcccgcga gacggtcaag gcgctgcgca agaacgtgac
    18001 ggccaaatgc tacggcggcg acatcacgcg caagcgcaag ctgctggaaa agcagaaggc
    18061 cggcaagaag cggatgcgcc agatcggccg cgtcgagatc ccgcaggaag ccttcatggc
    18121 cgtcctgcgg accgacgaag gctgaagccc tccggttttt cacaagctgt tccgacgtga
    18181 gcttccacga cgccttccgc aatccagacg tctacgactt gacagcgcca tccgcgtggc
    18241 aggcggttcc aacctcctga ccgcatcagt ccgcggagcg gacgaccgga attaagcaca
    18301 aggtgacggc cgccggccgg aaccccgcgt catctccccc atccccccaa acccccgcaa
    18361 gggggtggcc gtagattccg gcgcctggcg ttgtcgagag ccgaggacga gagacgagag
    18421 ccggaagccg agtttgttgc ccccccgctt accgcaactt caacatcgcc agacccgcca
    18481 tgctcaggat ccacgagacc agccagaacc ggataatgat cttgctctcg ggcatcccca
    18541 gcttctcgta atggtgatgc agcggcgaca tccggaaaat gcgcgttccc gtcagtttga
    18601 agcaacagac ctgcagcacc accgacagtt cctcgatcac gaacatcccg ccgatcacca
    18661 gcagcagcat ctcctgcttg agcagcacgg ccgtggtgcc gatcgccccg ccgagcatca
    18721 tcgagccggt gtcgcccatg aacacttcgg ctttcggggc gttgaaccac aggaaaccga
    18781 gcgacgagcc gagcagcgcg gcgagaaaaa caaccatctc gccaccgccg gggatatacg
    18841 gaacgtaaag atagcgcgag aagtcggcgc ggctcatcag ataggcgacg atcagaaacg
    18901 ccgcgatgtt ggcgatcgaa atgccgatgg cgagaccgtc caggccgtcg gtcaggttga
    18961 ccgcgttgga ggtcgccagc agcaccagca caacgaacgg aatgaagaac agccccagcg
    19021 gcagatagag gtacttgaaa aaggggagca tcaggaaggg atagccgctg gcgtcggcgg
    19081 gatgataata gaccccccaa tcgccggcat agagcgtgaa cgccaggacg acggcgagcg
    19141 ccgcctgcac cgcgatcttg cgccacggac tgaccccgcc gtgatgcttg cgcacgatct
    19201 tcaggtagtc gtcccagaac cccagggccc cgtagccgaa cgtcatcgtc agcaacagga
    19261 tgaagtggat attgttcagc cggcagagca gcagcgccgg cagcagcagc gagaagagca
    19321 tcagcaggcc gcccatcgtc ggcgtgccgg ccttggcgcc gtgcatcgcg gccagatcga
    19381 ccgcgttgtc gccgcggctg tggcggatca cctgtcctgc cttgagcgcc gccagccggc
    19441 ggatgaccca cggaccgatc agcagcgaga tggcgaaggc gagggccagc gccagcgcgg
    19501 cgcgcacggt gatgaacgtc agcacgcgcg ccggccccat gtcgtagtcc tccttgagca
    19561 ggcgttcgat caggtcgagg aacaggatca gcatggcgcg ggcgccgttc ttgtggactg
    19621 gaatgcgtcg tcagctcagg gattcaatgg ccgtcgggca ggatcgggac atcgggcacg
    19681 agcgcctgcg cgacgcgctc cagcgcgaaa ccgtgcgacc ccttgaacag gaccacgtcg
    19741 cccggccgga ctgtttcgcg cagcgacgta accagcgcgc tctgctcatc gaagcccgag
    19801 gccggccacc cttcggtgaa cagcatttcg gccaggtggg cgcaatacgt gcccaggccg
    19861 aagaacggcg tgggcccgat ctcgcgcagc gggccgagca gcatctcgtg cgccacttcg
    19921 ctctccgccc ccagctccag catgtcggcc agcaccagca cgcgccggcc catcagcggc
    19981 aggtgcgcca ggctctgcag cgcgccgagc atcgacggcg gcgaggcatt gtaacagtcc
    20041 aggatgagcg tcagctcgcc cgcccggacg acctgcccgc gcagcggttc agtatgaaac
    20101 tccggcagcg cgtcggcaat ccacgccgga tcgtagccgc cggccagcag cagcgcggcg
    20161 gccgcggcga cgttctccag gtgatgccgc ccgaacaggc gcagctccag atcccgcagt
    20221 tccccgcccg gcaactccag attgaagcgg taaccgacgt cgggcagcgc atgcacgggc
    20281 gagagcgcgc gcacctcgct gatccgcacc gccggctcga cttcgtagcg cgcgggccct
    20341 tcgcccagaa aatacgtgat cggataatcc ctggaaaact cggccatgcg catcatcgtc
    20401 cgcggacatc cggcgttgac caccagcggc cggccggcgg cggtgttgcg gaacaggtcc
    20461 agtttcgcgt cgatcaaccc gtcgaccgag ggaaacatgc cgacgtgggt caggccgatc
    20521 tgcgtcagca gcgccgcgtc ggggtcggcg atccgggtca gccgcgccag ctcgcccggc
    20581 tgattcatgc ccagttcgag caccgcgacc tgatgatccg cgttcaagcg caggagcgta
    20641 tgcggcacgc cgatcaggtt gttgtagttg tggagcggct ccagcgtggg gaaccggcgg
    20701 gccagcgccg cggcgagcag gcggcgggtg gtcgtcttgc cggcgctgcc cgagagcgcg
    20761 aggacacgcc cgccccagcg ccggcggcgg tcggcggcaa gctggccgta ggcgaacagc
    20821 gtatcctcga cgcgcaggat cggcaggtgg tcgggcaacg gatcggcggg ccagaaatcg
    20881 cgcgcgatga tcaagccggc ggcgccgcgc tcgacggccg cgccgcaaaa gcgatgaccg
    20941 tcgtgcgtct cgccgcgcag cgcgaggaag tactgccccg gcgcgatcgc gcgcgaatcg
    21001 gtgcagatcg cgccgaacgt ccgctcggcg tggattgggt cgtagcgggc gcccaggatg
    21061 gacgccgctt cagcgacggt cagggcttct agcggaggaa gtcccgcggg ggtcatggcg
    21121 cttcatgcgt gtcggggaga gggtctcgga ccacgcgact gcgcgggaat gcgccaccag
    21181 cataacccaa agccccaccg cgttggagcg aaaaggaggc gaacggcgga aagcctcaat
    21241 acttgcgcat cagcccgacg acgatgccct gaatccgcac gcgcgcggcg gggtagcgca
    21301 gtggcgacat cgtctcgttg gccggctgga gccggacgat ctcgccttcg ggataaaagc
    21361 gtttgacggt cgcctcggcg ccatcgatca aggccacgac cgtctggccg gggagcgcgg
    21421 agcggcggct ctcgacgacg agcagatcgc cgtcgcgaat accgtcgtcg atcatcgaat
    21481 cgcctcggac acgcaggacg aaactctccc gccggccgac cagttcgcgc ggaacatcga
    21541 tgtactccgg attctcaatc gcctcgatcg gcagcccggc cgccacctcg cccatcagcg
    21601 gcagcccatt gccccgcacg cgcgccttgc tggtcacctc aatcgcccgg ctgtgattcc
    21661 actgccggcg gatcagtccc ttctgctcca gattctgcag atgcttatgg accgtcgcca
    21721 gcgacgacag cccaagctgg tcggcgatct cctccagcgt gggggagtag cggttctcct
    21781 cgatgaactg ctcgatgacg ttgagcattt ccctctggcg gcgggtcagg tacatggcgg
    21841 cagctccccc ggatcatcca ggccggggcc cggcgtggcc ccgaccggcg gtgtcggcgt
    21901 tcagcatagg cgaagagatt acgaaaggaa agcgaaaacg gtggcgaact ttggatcacg
    21961 aatggcacga aatgcacgaa cgggcttcac tcgcggcggc gcgcggcgcg gaccgcccgc
    22021 tgatgttcgc gccacgtggc gctgaagatg tgctgatcgc cccccgcgta gacataatac
    22081 agataatccg tctcggccgg acgcagcgcc gcctccagcg ccgcccggcc ggggttggcg
    22141 atcggaccgg gcggcaggcc ctcatgccga tacgtgttgt acggcgaatc gacctccaaa
    22201 tcggcgtagg tgagcttgcg ctcccagacg tcgcccagcg cgtagtggac cgtggcgtcg
    22261 cactgcagct tcatccgccg ggccagccgg ttgtaataaa ccgaggcgat cagcggcatc
    22321 tccccggcgg agcgcgcctc gcgctggatc atcgaggcca gcgtcacgat ctgcctgggc
    22381 gtcaggtctt gcgccggcgc cccccgccgc gccgggtcgg ccgcccgcca ttgccgggca
    22441 aactcctcca gcatcgcctg caggatcgca cgcggcggcg aacccgcctc gatgaggtag
    22501 gtatcgggga agaggaaacc ctccagcagc ggggcgccgc cggcggccgc cagcgtgccg
    22561 ctggtcggtt cgatcccggt ggcgtcgatg aacgcgcgcg gcagctcgcc cggctgttcc
    22621 agaacactca gccacagcgc gccggtcgtg atccagtctt cgctggccag gcgctcgccg
    22681 atccgccgcg ccgtccagcc ctcggggatc gtcagcttgc gttgaaacga tccgcgccga
    22741 agctgttcgg tcagctctcg cggcgagatc gggccgcgaa gccgataccg gcccgcgtgg
    22801 agcgacgcca gcttgccctc gcgcttcagc cagagcaggt agagctccgg ccggctgacg
    22861 aggccgagct cgtgcatccg ccgggcgatc gcacgcgccg acgtgccggc gacgatcgtc
    22921 accgagcgct cggccgccga gccgtacggc cgctcgcccc accaggacag gtattcccat
    22981 ccccccacgg cggctcccgc ggtcagcagc gccagcagca gcagcgccgc ggcggtccag
    23041 cgcagccagc gccgccgcgg cgctgccggg gtcggggccg tcgtgggcga cggttcctgg
    23101 gtaggttcgg gcgtcatttc tctttcgcca gccattgatc gatgaaccgc tccaactgtt
    23161 cggcgagccg gggccaactg aattcgcgct ccgccagccg gcgggcggcg tggcccatcg
    23221 cttcgatccg cgccgggtcg tgaaacagct cggcgatcgc cgcggccagc gcgtcgggct
    23281 cgggcggcgc cgccgcgccg atgggcgccg ccgcgtcgcc gaaatagtcc gccaggtcgc
    23341 ccaccggcga cgtcaccacg ggccgccccg ccgccaggta gtcggtgaat ttgaacggca
    23401 tgcgcgcgcg gttatgctcg ttgtcctcca gcggcagcag cagcgcgtcg gccgcgccga
    23461 gcacgatctg catcctgtcg accggcaacc ggcccagctc gattaccgcc tcgcgcgcgt
    23521 gcaggtcgcg gatcagcgcc ggcgcgagcg gcggccccgc aacgaccagc cgcagctcgg
    23581 gcagccgctc gcgcgcccgc atcagcgtcc gcaccatcag ttcctggtcg ggatgataat
    23641 tggcgatgta gcccagcagc ggggcgtcgg ccggcaggtt cagctcgcgg cggcaggcgc
    23701 cgcgctcctg ggggcggatg ccgtccaggt tggccccgtt gggcagcagc aggatctccg
    23761 cctcgcgccg gcccagcgcc agggcgacct cgcgcaggcg gcgcgaaatg accgtcagcc
    23821 ggcccgccgc gcgcatcacg cgccgttcca gggcgagttc ccagcgctcg gcgcgcgcct
    23881 gcaggaactt ctcgcccggg gggatcagcc cctcgcgccg catccgttcg cgcttgggga
    23941 acacgccccc cgcgtaccag tcgcaccagt cggccagcag cggccggccg cgcagccggt
    24001 gcgccacgaa cgccggcagc gccacgttgg gcggatgcgc gaacgcgtag acaaggtcgt
    24061 ggggatcgag cagcgcccga cccgcgcgcc aggcggcgtc gagcggcccc cagccgtcgt
    24121 cggccccggc cagcggcgcc cagctcggcg tctcaacaat ccgcacgccc gcctccgtca
    24181 tgcgctgggg acggtagaaa tgccgcggcg ccgcggtcca cagcgtcacc gcatggccgc
    24241 gcccggccag ctcgcgcgcc agccgccagg cccggtgcca cgtgccgcag ccgcgcagat
    24301 tgtgattgag catcaggatc cgcaatcgct cgctccccag ccgccgcgca cttctgtccg
    24361 tctccgccgg tccatgttcc ctcggcctca aatcagcagg atcgccattt gcagcaggcc
    24421 gatcagaaag cccagcacgc cgcccaggac ctcgatcgcc ttcagttcgc gccgggcgat
    24481 ctcaaagatg atcgtctcga gccggacgac atcgaactgg cggacttttt cctcgaccag
    24541 cgcgccgaaa tcgagctcgt cctccagccg gtcgatcacc tggccgatgg cgcgcggcgc
    24601 cagccgccgt gcctcggcca ccagcagccg ttcgaccttg cgccgcagtt ccccgcgcag
    24661 catcacgccc agcagcggat tgagccggcg caaccggcga tcgacaaacc gattgaactt
    24721 gtcgcgcact agcccgtcga tcgcatcctg cgtccgctgc cccagcagga tcgactcgat
    24781 gtcgtcatgg gagatcaggt ggcgttccac cgtctggccg atgctgacgg ccagctcgcg
    24841 ctggcgcttg ggcaccagac cctgcagcgt gaagccgagg atgcgccgcg gccggcgcgg
    24901 ccggaagatc atcttgaccg ccagccagtt ggtcgcccag ccgatgagtc cgccgaccac
    24961 cgggatcatc gccagccgcg cccagtcctg ccactgcgtc aactcgaaca tgcgccgacc
    25021 ttccgccgtc acatcctagc ggggagggcc gcccgccgaa tagcgcgaag tgccgaccgg
    25081 gttgaatctc gttcgcattc gccgccgaac cgggtaaggt gggaggttgt g
//
LOCUS       DAOPCO010000031        24397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2203_length_24397_cov_6.340194, whole genome shotgun sequence.
ACCESSION   DAOPCO010000031 DAOPCO010000000
VERSION     DAOPCO010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 24397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24397
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2203_length_24397_cov_6.340194"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..1368
                     /locus_tag="PLS90_04140"
     CDS             103..1368
                     /locus_tag="PLS90_04140"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPK01627.1"
                     /translation="MNKPSAPAAEYPANIPFLDNAGWRDRYDKLFTPNYGARRLALVR
                     GEGTRVWDADGNEYLDFLTGISVNNLGHCHPRITAAIREQAATLVHCSNHYYIPQQIE
                     LAQMLIDHSFADRLVFGNSGAEANEAAIKIARRWQKETRGPGHHEIISFTGSFHGRTL
                     ATLTATGQEKVKLNFDPLPEGFVHAEYNNLDSVRAALSDRTGAILVEPITGEGGIHIA
                     TNEFLRGLRELCDERGLLLIFDEIQCGLGRTGTLFAYEQYGVEPDIMTLAKSLGGGLA
                     MGVMLCTEAVAGAFAPGAHGSTMAGNPLTAAAGRAYLAELIEGGWPGKARRMGEKILQ
                     RLRETTAGMRRVKEVRGRGLMLAVELTDGAPDVLLACERAGLLVNVTAGQVVRLLPPL
                     NVSEAEVERALAILVEAIATAPAAAGGAA"
     gene            1365..2312
                     /gene="argF"
                     /locus_tag="PLS90_04145"
     CDS             1365..2312
                     /gene="argF"
                     /locus_tag="PLS90_04145"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPK01628.1"
                     /translation="MSPIRSSKRDLRTSDDLAPDEIDEVLRLATELKSELREGAPRPL
                     LAGRALAMIFEKPSLRTRCTFELGVFQLGGHAVNLTHREIGLGQREPVGDIARNLERW
                     FDLVMARTFRQATVDELAARCRIPVINALSDFEHPCQALADFLTLRERVGDLRGFNLT
                     YIGDGNNICHSLIRIGVRLGVNVTVSSPAGYEPDPAVAEAAARDAACGGGAFHFTPDP
                     RQAVARAQAVYTDVWASMGQEDEAAQRARVFAPYQVNAELLAAAPTDALIMHDLPAHR
                     GEEITADVIDGPRSIVFDQAENRLHAQKAVMVFLHNHAG"
     gene            complement(2400..4235)
                     /gene="recQ"
                     /locus_tag="PLS90_04150"
     CDS             complement(2400..4235)
                     /gene="recQ"
                     /locus_tag="PLS90_04150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPK01629.1"
                     /translation="MHHLTQILSRYWGYSSFRPHQREAMEAVLAGRDSLVVLPTGGGK
                     SICYQAPALAMEGMAVVVSPLISLMKDQIDALVGCGVPAACLNSAMPFDERRAVFAAV
                     RQARLKILYVAPERLLLDGFLDALRQARISFFAIDEAHCISQWGHDFRPEYRRLRQLK
                     EAFPAVAVHAYTATATHRVRDDIARQLGLAAPEVLVGSFHRPNLIYRVEPRAAGDGFL
                     DQIAELVGRYRGESGIVYCLTRKKVEQLADHLRARGVAAAPYHAGLEDHVRRRNQEDF
                     INDRIQVIVATVAFGMGIDKSDVRFVIHAAMPRSLEHYQQESGRAGRDGLDADCVLLF
                     SPRDYQFWKRMLEEQPGETQRSAQATLSAMFDYCEATRCRHRALLAYFGEELGRPDCG
                     SCDVCLGGVETVPDALVIGQKILSSVVRQGEGHDALHTARVLGGSANACVLAAGHDRL
                     STHGLLKDERRETVLDWIAQLVRLGCLEQREGDGALAVTPAGRRVLRGEATPRLLRPA
                     PARSANVDGYLRRRRGDDAAWRGVDTDLFDRLKTLRRTLAEARAVPAYVVFSDATLRE
                     LARRRPATRKALLKISGIGERKAREFGDEFLKLIRDHRRADEPVG"
     gene            complement(4539..4769)
                     /locus_tag="PLS90_04155"
     CDS             complement(4539..4769)
                     /locus_tag="PLS90_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01630.1"
                     /translation="MTDLLTKAFKKASQLPAEQQDQLARELIAELEGDQLWEASFARS
                     QGQLEQLARKALQEHEAGKIWASTSFEIAADP"
     gene            5015..6895
                     /gene="typA"
                     /locus_tag="PLS90_04160"
     CDS             5015..6895
                     /gene="typA"
                     /locus_tag="PLS90_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPK01631.1"
                     /translation="MTAADKIRNVAIIAHIDHGKTTLIDAIFRAAHVFRENQQVAERV
                     MDSNVLERERGITIRSKHCTVTWRDYLINIIDTPGHADFSGEVERVLSMVDSVLLLVD
                     ANEGPMPQTRYVLMRALRLGLRPIVVVNKVDRPNARPELTLNKTFDLFIELGANDEQA
                     DFPVVYGSGLGGWLVRDLEHDAREGMEALFETIVTHVPPPRVDADAPFLMQVSTLAWS
                     DYIGQIGCGRVLQGRLQKGEAFTQVVTRWREAGNRDAGWDVLGSSSERAVHLWVTRGL
                     ERVDVDEIGAGDIVWIAGPAEIRIGDTLAAAGRDDLALPPLEIEEPTVSMFFLVNNGP
                     FAGEEGKAITLRQLKERLERELRTNVALRVEDLGRADGVKVSGRGELQLAILIEEMRR
                     EGWELCVSRPEVITHTGPHGEVLEPMEQLIIDVPEEYQGVVIEKLARRKGQLTHMENS
                     GTGTLRLEFAIPTRGLIGYRNEFLTDTRGLGIMSSRFTGYGPWQGEVAGRNRGSLVSM
                     QTGPATAYQLESLQARAVLFIGPTEPVYEGQIVGMNSRPEDLPCNPTKKKALTNHRAS
                     TKENNEGLNVPRRMSLDQALEWIADDELVEATPKSIRLRKMILNAEERKKDERYKVAL
                     SA"
     gene            complement(7005..9572)
                     /gene="gyrB"
                     /locus_tag="PLS90_04165"
     CDS             complement(7005..9572)
                     /gene="gyrB"
                     /locus_tag="PLS90_04165"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013943755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPK01632.1"
                     /translation="MGKKPDTIEPAATPAPREYTAKDIQVLEGRAAVRKRPAMYISNT
                     GALGLHHLVYEVIDNSVDEAMAGFCNRIDVTIHFDNSVSIADNGRGIPTEEHAAKKGK
                     STVEVVMTILHAGGKFDNEAYKYSAGLHGVGVSVVNFLSDWLEVEIKRNGKIHFMRFE
                     KGITKEPLHEIGASKKTGTLVRFKPDPDVFETLDFSFETLATRFRELAFLNAGLTINL
                     IDERTGKERSWNYKGGIMEFVRQLNENKKPIHPKPIYFNRSRQFERKDRETGQTRLDT
                     MEMEIAVQYNDSYDENVMTFANNINTRDGGTHLSGFRKALTRTINDYAQRNDMLKKLK
                     GSLTGDDMREGLTAIISIKISNPQFEGQNKGRLLNPEVAGWSEQMVNESLAEFLEENP
                     AVARRIVDKVVNAAEARMAARKAREIVRKSAMDITALPGKLADCSEKDPSLCELYLVE
                     GDSAGGSAKQGRDRHFQAILPLRGKILNVEKARLDRILSNNEIRTMVTALGTGIGADN
                     FDLSKLRYHKIIIMTDADVDGSHIRTLLLTFFFRQMRELVAGGYIYIAQPPLFRVKKG
                     KQEQYLDNEEQRDRFLLNLSVDDIVVEYTARGNGHRPVEVSKAALRAMLEDVIALEGL
                     RHILQRKGLPLHEYIRMRDDGGRLPRYQVSLDAEVRYAYSEKQLAALIAELEETGHDE
                     EPVAELDQPDLLDQADAALEEPKPRHTLVELPEADAVEAILTRVEKLGIPADLVVHQE
                     FEIDPKNPFEEYHPFQVRAGDGRAMAADSLPQVLERVREIGSKGVQVQRYKGLGEMSA
                     SQLWETTMNPAKRRLMQVRLEDAATADQIFTILMGDEVDPRRRFIQKNAPEVRNLDI"
     gene            complement(10215..10817)
                     /locus_tag="PLS90_04170"
     CDS             complement(10215..10817)
                     /locus_tag="PLS90_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01633.1"
                     /translation="MIYLDAVLGLILVILLAGAFGRYPLWRLALEALVGLAMIVAIHA
                     LAAMLWRWLANHLDEGATPPSRRRMGSTFARKTRRETRFEALDDQELERHLERHPDDA
                     LALEIHCERLWAAGRRDDYARELSWLLNLPNRMSIDEQVTRLHWLADYHLRENRRPGQ
                     AIEALRLIEARFPGTYQATLARQRILSLRTALAEGGKAGS"
     gene            complement(10839..12434)
                     /locus_tag="PLS90_04175"
     CDS             complement(10839..12434)
                     /locus_tag="PLS90_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HPK01634.1"
                     /translation="MPRRVSLFLLLIALAPLGCRPPSPRAERQATHQAVINLGTDPRT
                     LDPSLATDVASARALLFFQRGLFMLDDEGKPRPELAESWEVSPDSRIYQIKLREAYWT
                     NGQRVTADDFLYTWTERVLNPEFGAEYAHQIYNYIAGARAYYENPELGTETVRVWALA
                     PDQLYVELEHPTPYFLELLAHHAYFPVCRAVDEQNADWARRAETYVGCGPFVLAEYQP
                     GARIVARRNPQYWNAAAVKMEQVTLRMIEEESTARIAFENGEIDATEGVPRAELDSLR
                     GSEALAFSTAFSTYYLNLNCQKEPLRDVRVRRALALALNRREITEKVTRAGEQPAFFI
                     SPPGLYEAPPAPTFADADFDEARRLLAEAGYPGGQGFPRLRYIYNMTDTHQLIAQVIQ
                     ETWRRELGIEMTVENQEFKTLIENRRQGNFDVARAGWVADFPDPLNFLEIFASDSDTN
                     DSFWRDPAYDALLFQARAETDPDTREAILRQADLYLIEQMPIVPIYIYSTPYLRDPAL
                     EGFHLNPMAMFDPVRLAWRPPPS"
     gene            12866..13942
                     /locus_tag="PLS90_04180"
     CDS             12866..13942
                     /locus_tag="PLS90_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020526408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01635.1"
                     /translation="MAGNLRAIVIGCGNMGASHARAYAALDGFELVGLVARSPTRRGK
                     LAAGLSPAAEFATLEAALAATRPDVAAVCTYPDSHAAYCRQCLEAGLHVFVEKPLAMT
                     VEEAGEVVQIARRRGRKLVVGYILRHHPAWIRFVEIARTLGKPLVMRMNLNQQSVGAT
                     WDLHKNLMESMSPIVDCGVHYVDMMCQMTRSRPVRVQAIAARLTDEIAPTMTNYGQLQ
                     VVFADGSVGWYEAGWGPMISEEAYFVKDVFGPKGSVSIVKGRDGEQAHQSANLESHTQ
                     TNWLRVHHADQDAARQFTREDEWIDTTDEPDHQELCNREQAYLLRAIRDDLDLTDHWA
                     DAVNSLRIVLAADEAARTGRTIEL"
     gene            14024..15331
                     /locus_tag="PLS90_04185"
     CDS             14024..15331
                     /locus_tag="PLS90_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01636.1"
                     /translation="MNEKRISRRQLLRRTLKCSAAAGLFTIVPRHVLGGPGYTPPSEQ
                     LTKGVIGVGSMGRTHLSYGPSRLLAICDVDEQHLEGAALHLRENLGLAERQVREIRRY
                     RDFRELLAQPDIDIVHIATPPHWHALIAIAAARAGKDIWCEKPMTRTIGEGRAVVEAV
                     RRHGRVFRVNTWFRFTDPFYAFGSRCQPIKQLVDSGLLGWPLTVTVGLATGFSWKFLW
                     QGRTDLTPQPVPVHLDYDLWLGPAPFKPYHPHRVHETFRGYWDYDGGGLGDMGQHYLD
                     PVQFLLGKDDESPVRVEADAPQQHPDAVGIFRRIEMTYADGCRIILDGSESETRTPYI
                     EGPNGRLYRGFECDVPGWREVLATRPDPAPQRDDFEQAVRERRKFALNEANAHRSCTL
                     VNLAKAAVYLGRPLRFDPVRQEFIDDPAANRLINPPMRAPWHL"
     gene            15349..18771
                     /locus_tag="PLS90_04190"
     CDS             15349..18771
                     /locus_tag="PLS90_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK01637.1"
                     /translation="MTESIRAGLLAAACALYLTGCALFAPSGPRMPAAERGAALAAVV
                     ARMPADAAAQANADFAELVRLGPEAVRMLCEQLATAEPGEAAGVQYALHGLAQYVGRP
                     GAGRERRMFARAVSAALPGTADPQAKNFLIRLLETIGGDDAVAALAPLAHDPASGDAA
                     AQALAAIGTPRAREALIAALATAPPDRRAALAQALGYTRAPAAADALRPLATDADNAV
                     RRAALAALADLGDPADAPLLAAAVPDADDPAADWAADNQLRYARRLAEEGHDRAAREI
                     CARWLDPAGARPTQFRCAALALLTDIEGEAANPALLAALDDPDPQFRAAATWLALELK
                     GDAVTESWAARLATTADPAARLDVLEMLTARGDRAALPAVLARMEDEEPAVRLAAITA
                     AGRLGGAEAAPALIAALGRTDRAEAEAARAALIQIPGEDHLDALARAYADADEPTRRG
                     TLAILARRGATQHADLARAALTDDAAPLRLAALDALADLGSADDLPRLIERLIQAPTQ
                     SEAAAARRAIVAFARRTGAEADVAERLLQELDGREQDGQPVLLTTLGNLGSPAGLPRL
                     REAAGDEHPAVRDAALRALFDWPDPACAEDLLTVMRENPEPKYRTLAARALARLAATP
                     DLKAAERLDLCRKALAAIEDPAAGKPFLALLAEIPTVESFRLAASYLEHEATKAEAAL
                     AVAELSPRVCQEIPGEVHAALAGAADAIADEGLRERALTLMEKIGAADAVSLFNGRDL
                     TGWIGDTAGYAVERGVLVSTESGRNLFTEREYGDFVLSFDFQLSPGANNGLGVRAPLE
                     GDAAFEGLEVQILDDTAPQYAELKQYQYHGSVYGIVPAERGHLKPVGEWNHEVVIARG
                     RWITVYLNGHRIVHADLDQASREGTMDGREHPGLARATGHLGLLGHGSRVAFRNLFLR
                     ELTPGEPAADAYPHETADLGIVPPPAGFTALFNGRDLTNWQGLPAEPNDNPLRRAELS
                     PAELAAARQAADAEMRAHWTVADGVLRFDGNGRSLCTARDYGDFELLVDWKIEPLGDS
                     GIYLRGVPQVQIWDSNQWFGIGSGGLYNNQGHPSRPLVCADRPVGQWNTFRIRMEGDR
                     VTVHLNGVRVVDDVVLENYWDRKRPLWPRGPLELQSHATKLWFRNIFIRELH"
     gene            complement(18773..19816)
                     /locus_tag="PLS90_04195"
     CDS             complement(18773..19816)
                     /locus_tag="PLS90_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01638.1"
                     /translation="MSTTYSRREADQLYFEQHFEDWLPRRLFDLHAHLVPPGTLRAPS
                     PERLARYWMLRLPLQQTVQEFFEDYAALLPGRQVHALAFGMPVQESDIARQNAMLLEG
                     SRQHERLNVLFLTRPEDEEERLEQALADGFLGFKPYPDFVVGDHPGEEAIAEFLTPAM
                     CRVAQRHRALVMLHISRPGRFGDPRNVDDLLQLAADYPAMRLIIAHLGRAYNPIYLER
                     ALERLDGRHPWWYDFSAVVHPGTHRLALQSIPHERICFGFDNPIMLARGYYEFPTEST
                     YRVRMHGIDLDEADHPPLAYQILKGFKEAAESVRLDRASLERILFQNAWDLVHGARAD
                     AARGAVGAAATGG"
     gene            complement(20044..21573)
                     /locus_tag="PLS90_04200"
     CDS             complement(20044..21573)
                     /locus_tag="PLS90_04200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK01639.1"
                     /translation="MTMTADAVIGIDVGTTNFKLLVLDAAGARLAESSVTYPLDMGPG
                     GRVTQDPELWWTSLEECLARVGRQMDLRRVGALALSSQGETLICCDDTGRALAPAISW
                     MDTRAAATTGELQRLRDDWTARTGKPIATYCNLAKIHWLRRHRPELIARTRRFCQVAD
                     YLIYRLTGRWVCDLNVASFSSAANVWERGWDVELCRDFDLEDKLPEIGEAGTIVGPLR
                     PELRQRWGLRDDVQLVLGGHDQGCAVLGAGPPTVGSVFLSAGTAWVLYAPTRRPAPSA
                     ESNAVRYCHAWPDLWATLASYTGCGALNHWIDRFCRAERFEAEQQGRAVYDWLLRDEN
                     LAEDLIVIPYLFGAVCPDNDPAARAALLDLEPAHTPANVACGFALAVVFETRRNLECL
                     RSLGVDPGGLVMSGGGARGRVWPQWVADACRLPISVMRHSDTAPLGAAFLAGCALGFW
                     PDGRLPDYPIARRCEPRADRAERFDALYERYLRAVEMDRRRRRPEPADTPAEVLSIPS
                     A"
     gene            complement(21570..23021)
                     /locus_tag="PLS90_04205"
     CDS             complement(21570..23021)
                     /locus_tag="PLS90_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01640.1"
                     /translation="MSTVIDPQAIEQDAIEQGEPPAPPRLGLLSLSLDLYRKVVPEAL
                     ATYCASHAQRVEVLGRYGEVVGHDLCCNREEVERTVARIQAAGADVLVVVLQSYSLSL
                     ASLPALLRTPLPLVIWNTQDLGSIDDGFDADRMMANHGMHGVQDLCSCLRRTGRTFGL
                     VSGHWRNEAHLAELADWLKAAHGVALSRRMKVGLMGRPFAGMGDFTFDEVGLISRWGP
                     AVEPLTLAELAEAQARIGEAELAAEIEADRERFAVEPDVTDEDHRRSARLAIALRRVV
                     EAHGVDAFTMHFGVFGEDPRIETCPFLGVNHLIAAGRGYAGEGDATMAALDGLLGRLY
                     GPAGFCEMFTADFEHNQVFMSHMGEGNYRLARPGERPVLRKVPYTFGKGRPFLVPLFR
                     YAPGPTTLVNVAVRGDGRFGLIAFEADVLDFNLGERYDSPHFKIAVKGELGPLLDRYS
                     RAGGTHHLTRIPGRRLDSLERLAHMLEMEFTRI"
     gene            complement(23118..24143)
                     /locus_tag="PLS90_04210"
     CDS             complement(23118..24143)
                     /locus_tag="PLS90_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK01641.1"
                     /translation="MSVRLQDIAREAKVSYATVSRVLNNHKNVRAQTRTRVLEVLRRH
                     QYGVKRSRARALSTEFNIELIFHRAWQNNTAGAQYFASVIAGAEAILQRLNCHLIVRT
                     VSSGRLDSYFESLSADRSIDGVIIVYYDAEYQELYRRLQEQRIPCVLLQPRLLVEEAI
                     TVFPDNLGGAQRAVEHLIALGHRRIAHLHSVDGDYEATERRWAYELALRDAGIPLTAE
                     YLQTTGGWDAESGEQAMGRLLRLAAPPTAVFAANDSIAIGAMRACAAHGLKVPEDVSI
                     VGFDDFYPEGFMMPALTTINYPIPTLVEVAIREVLDHLQHRDFHAQRILTPVRLMERQ
                     STRRLSE"
BASE COUNT         4002 a         8209 c         8319 g         3867 t
ORIGIN      
        1 accggaccgt cccggtcttg acgccgccgc gcgtttctgg ataatcgtct gtttcccttc
       61 gcctggcagg cccagcgcat cccatgccgg ggggttatcg acatgaataa accgtctgct
      121 cccgccgccg aatatccggc caacatcccg tttctggaca acgccggctg gcgcgaccgc
      181 tacgacaagc tgttcacgcc caactacggc gcgcggcggc tggcgctggt ccgcggcgag
      241 ggaacgcgcg tctgggacgc cgacggcaat gagtatctcg atttcctgac cggcatcagc
      301 gtcaacaacc tgggccattg ccatccgcgc atcacggcgg caatccgcga acaggccgcc
      361 acgctcgtcc actgctccaa tcattactac atcccccagc agatcgaact ggcgcagatg
      421 ctgatcgatc acagcttcgc cgaccggctc gtcttcggca actcgggcgc cgaggccaac
      481 gaggcggcga tcaagatcgc ccgccgctgg cagaaggaga cccgcggccc gggccaccac
      541 gaaatcatct cgttcaccgg ttcgttccat ggccgcacgc tggcgacgct gacggcgacc
      601 ggccaggaaa aggtcaaact gaacttcgac cctctccccg aggggttcgt ccacgccgaa
      661 tacaacaatc tggattcggt ccgcgcggcg ctcagcgacc ggaccggcgc gatcctcgtc
      721 gagccgatca ccggcgaggg ggggattcac atcgccacca atgaattcct gcgcggcctg
      781 cgcgagctgt gcgacgagcg cggcctgctg ctgatcttcg acgaaatcca gtgcggcctg
      841 ggccggaccg gcacgctgtt cgcctatgag caatacggcg tcgagcccga catcatgacg
      901 ctggccaaga gcctgggcgg cggcctggca atgggcgtga tgctctgcac cgaggcggtg
      961 gcgggcgcgt tcgccccggg cgcgcatggc tccacgatgg cgggcaaccc gctgaccgcc
     1021 gccgccggcc gcgcctatct ggcggaactg atcgagggag gctggccggg caaggcgcgc
     1081 cggatgggcg agaagatcct ccaacggctg cgcgagacga ccgccggaat gcgccgcgtc
     1141 aaggaagtcc gcggccgcgg cctgatgctc gccgtcgaac tgaccgacgg cgcgcccgac
     1201 gttctgctcg cctgcgaacg ggccggcctg ctggtcaacg tcacggccgg gcaggtcgtg
     1261 cggctgttgc cgccgctcaa cgtcagcgag gcggaggtcg agcgcgcgct ggccatcctg
     1321 gttgaggcga tcgccaccgc gccggccgcg gcggggggcg ccgcatgagc cccatccggt
     1381 cctctaagcg cgacctgcgc acgtcggacg acctcgcccc cgacgagatc gacgaagtcc
     1441 tgcgcctggc gaccgagctg aaaagcgagc tgcgcgaggg agcgccgcgg ccgctgctgg
     1501 cggggcgcgc cctggcgatg atcttcgaaa aaccctcgct ccgcacgcgc tgcacgttcg
     1561 agctgggcgt cttccaactc ggcggccatg ccgtcaacct gacccaccgc gagatcggcc
     1621 tcggccagcg cgagccggtc ggcgacatcg cccgcaatct ggaacgctgg ttcgacctgg
     1681 tcatggcgcg cacgttccgc caggccaccg tcgatgaact ggccgcccgc tgccgcatcc
     1741 ccgtgatcaa cgcgctgtcc gacttcgagc acccgtgcca ggcgctggcg gatttcctga
     1801 ccctgcgcga acgggtcggc gacttgcgcg gcttcaacct gacctacatc ggcgacggca
     1861 acaacatctg tcattcgctg atccgcatcg gcgtgcgcct gggcgtcaac gtcacggtca
     1921 gttcgccggc gggctacgag cccgacccgg cggtggccga ggcggccgcg cgcgacgccg
     1981 cctgcggcgg gggcgccttc cactttactc ccgatccgcg ccaggccgtg gcgcgcgctc
     2041 aggccgtcta caccgacgtg tgggccagca tgggccagga ggacgaggcg gcccaacgcg
     2101 cgcgggtctt cgcgccgtat caggtcaacg ccgagctgct ggccgccgcg ccgaccgacg
     2161 cgctgatcat gcacgacctg ccggcccacc gcggcgagga aatcaccgcc gacgtgatcg
     2221 acggcccgcg gtcgatcgtc ttcgaccagg ccgaaaatcg cctgcacgcc cagaaggccg
     2281 tgatggtctt tctgcacaac cacgccggct gacgcccgcc gcggccccgt cgttgcccgt
     2341 ctcacccccg cgccgcgtga aaaagcgaag cgaacccagg gaaaagtcag ccaaaaaagt
     2401 cagccgaccg gttcatccgc gcggcggtgg tcgcggatca gtttcaggaa ctcgtcgccg
     2461 aactcgcgcg ccttgcgctc gccaatcccc gagatcttca gcagcgcctt gcgggtggcg
     2521 ggccggcggc gggcgagctc gcgcagcgtc gcgtcgctga aaaccacgta ggccggcacc
     2581 gcgcgcgcct cggcgagggt gcggcgcagg gttttcagcc ggtcgaacag gtcggtgtcg
     2641 acgccgcgcc acgcggcgtc gtcgccgcgg cggcgccgga ggtagccgtc gacgtttgcc
     2701 gacctggcgg gggcgggccg cagcaggcgc ggggtcgcct cgccgcgcag gacgcggcgg
     2761 ccggcgggcg tcaccgccag cgcgccgtcg ccctcgcgct gctccaggca gccgagccgc
     2821 acgagctgcg cgatccagtc cagcacggtc tcgcgccgct cgtccttgag caggccgtgc
     2881 gtgctcagcc ggtcgtggcc cgccgccagc acgcacgcgt tggcggagcc gcccagcacg
     2941 cgagcggtgt gcagcgcgtc gtggccctcg ccctggcgga caacgctgga gaggatcttc
     3001 tggccgatga cgagggcgtc gggaacggtc tccacgccgc ccaggcagac gtcgcacgac
     3061 ccgcagtccg gccgcccgag ctcctcgcca aagtaggcca gcagcgcccg gtggcggcag
     3121 cgggtcgcct cgcagtagtc aaacatcgcg ctcagcgtcg cctgcgcgct gcgctgggtc
     3181 tcccccggct gctcctccag catgcgcttc cagaactggt aatcgcgcgg ggagaaaagc
     3241 agaacgcagt cggcgtcgag cccgtcgcgc ccggcccggc cgctttcctg ctggtagtgt
     3301 tccagcgagc ggggcatcgc ggcgtggatc acgaagcgaa cgtcggactt gtcgatgccc
     3361 atgccgaagg cgacggtggc gacgatgacc tggatgcggt cattgatgaa gtcctcctga
     3421 ttgcgccggc ggacgtggtc ctccaggccg gcatgatacg gggcggcggc gacgccgcgc
     3481 gcgcgcagat ggtcggcaag ctgctcgacc ttcttgcgcg tcaggcagta gacgatgccc
     3541 gactcgccgc ggtagcgtcc gacgagctcg gcgatctggt ccaggaagcc gtcgccggcg
     3601 gcgcgcggct cgacgcggta gatcaggttg ggccggtgga acgaaccgac gaggacctcc
     3661 ggcgccgcca ggccgagctg ccgcgcgatg tcgtcgcgga cgcggtgcgt cgccgtcgcg
     3721 gtataggcgt ggacggcgac cgccgggaag gcctccttga gctggcgcag gcggcggtat
     3781 tcgggccgga aatcgtggcc ccactggctg atgcagtggg cttcgtcgat ggcgaagaac
     3841 gagatgcgcg cctggcgcag cgcgtcgagg aagccatcga gcagcagccg ctccggcgcg
     3901 acgtagagga tcttgagccg cgcctggcgc acggcggcga agacggcgcg gcgctcatcg
     3961 aacggcatcg cgctgttgag acaggccgcc ggcacgccgc agccgaccaa cgcgtcgatc
     4021 tggtccttca tcagcgagat cagcggcgag acgacaaccg ccatcccctc catcgccagc
     4081 gcgggggcct ggtagcagat cgacttgccg ccgccggtgg gaaggacgac gagcgaatcg
     4141 cgccccgcca ggaccgcctc catcgcctcg cgctgatgcg gccggaagct ggagtagccc
     4201 caatagcgcg acaggatctg ggtgagatga tgcatacagg ggtcagcggg gatgcggcgg
     4261 aatgccgatt tggcggacgt agccgtcagt ggccctcata gccgggaagt cagggtgtcg
     4321 taatcggcat gcgatccgat ccagaaccag atgatcgtgt cgccgtttac caccccagga
     4381 gctcgccagc ggatcccgat cctcaccgaa tagacattgg ttttgacccc aattcgtttg
     4441 aagcgaaggc tggggtggga ggggttcacc ttccagattg cgtaagttgt ccgcgccaac
     4501 tgctgtatgg gaggcggcgg cgaaagagca ggatgaagtc acggatcagc agcgatctca
     4561 aagctcgtcg aagcccaaat cttgccggct tcgtgctctt gcagagcttt ccgcgccaac
     4621 tgctcgagct gaccctggct gcgggcgaag gaggcttccc agagctgatc gccttcaagt
     4681 tccgcgatga gttcgcgagc gagctggtcc tgttgctcgg cgggcagttg cgatgccttc
     4741 ttgaacgctt tagtcagcag gtcggtcatg ggaaggcctc cgtttcatgg gcatgcgaat
     4801 acgagaaagc ctggatcgcg atgcgggtca tgtttccggt cccagggggc accaaccatc
     4861 ctcagtaaat catggaacgc gcctgccctt caaggcgcgg cgggtggggc aattcgcatt
     4921 ggccagaggc ggggcgattt tggtagaatg ccggacgtct gcgccgttca cccggcggcg
     4981 cgacggatac cccccgaaga ggatccccca ctggatgacc gccgccgaca agatccgcaa
     5041 cgtggcgatt atcgcccaca tcgaccatgg caagacgacg ctgatcgacg cgatctttcg
     5101 cgcggcgcat gtgttccgtg aaaatcagca ggtggccgag cgcgtgatgg acagcaacgt
     5161 gctggaacgc gagcgcggca tcacgatccg ctcgaagcac tgcacggtga cctggcgcga
     5221 ctacctgatc aacatcatcg acacgccggg ccacgccgat ttttcgggcg aggtcgagcg
     5281 cgtcctgtcg atggtcgact cggtcctgct gctggtcgac gccaacgagg ggccgatgcc
     5341 gcagacgcgc tacgtgctga tgcgcgcgct gcggctcggg ctgcggccga tcgtcgtcgt
     5401 gaacaaggtc gaccgcccca acgcgcgacc ggagctgacg ctcaacaaga ccttcgacct
     5461 gttcatcgaa ctgggggcga acgacgagca ggccgatttc cccgtcgtct acggctcggg
     5521 cctcggcggc tggcttgtgc gcgacctgga gcatgacgcg cgcgagggga tggaggcttt
     5581 gttcgaaacg atcgtgacgc atgtcccgcc gccgcgggtc gacgccgacg cgccgttcct
     5641 gatgcaggtc agcacgctgg cgtggagcga ctacatcggc cagatcggct gcggccgcgt
     5701 gctgcaaggg cgcctgcaaa aaggcgaggc gttcacgcag gtcgtgacgc gctggcgcga
     5761 ggccggcaac cgcgacgccg gctgggacgt gctgggcagc tccagcgagc gggccgtgca
     5821 tctgtgggtg acccgcggct tggaacgggt cgacgtcgac gaaatcgggg ccggcgacat
     5881 cgtctggatc gccgggccgg cggagatccg catcggcgac acgctggccg ccgccggccg
     5941 cgacgacctg gcgctccccc cgctggagat cgaggagccc accgtatcga tgttcttcct
     6001 ggtcaacaac ggcccgttcg cgggcgagga gggcaaggcg atcacgctgc gccagctcaa
     6061 ggagcggctc gagcgcgagc tgcgcaccaa cgtcgcgctg cgcgtcgagg acctggggcg
     6121 cgccgacggc gtcaaggtct ccggccgcgg cgaactgcaa ctggcgatcc tgatcgagga
     6181 gatgcgccgc gaggggtggg aactgtgcgt ttcgcggccc gaggtgatca cccacaccgg
     6241 cccgcacggc gaggtgctgg agccgatgga gcagctcatc atcgacgtgc cggaggaata
     6301 ccagggtgtc gtgatcgaaa agctggcgcg gcgcaagggc caactgaccc acatggaaaa
     6361 cagcggcacc ggcacgctgc gcctggagtt cgcgatcccg acgcgcggcc tgatcggcta
     6421 ccgcaatgaa ttcctgaccg acacgcgcgg gctgggcatc atgtcctcgc gcttcaccgg
     6481 ctacggcccc tggcagggcg aggtggccgg gcgcaaccgc ggatcgctgg tttcgatgca
     6541 gaccggaccg gccacggcgt atcagctcga gagcctgcag gcgcgggcgg tgctgttcat
     6601 cgggccgacc gaaccggtct acgaggggca gatcgtcggg atgaactcgc ggccggagga
     6661 cctgccctgc aacccgacca agaagaaggc gctgaccaac caccgcgcct cgaccaagga
     6721 gaacaacgag ggattgaacg tcccgcgccg catgtcgctc gatcaggcgc tcgaatggat
     6781 cgccgacgac gaactggtcg aggcgacacc caaatcgatc cgcctgcgca agatgatcct
     6841 caatgccgag gagcgcaaga aggacgaacg ctacaaggtg gcgttgtcgg cctgaacagt
     6901 ggatatgttt tcgcgaagtt gaacggagct gaggggcgcg aaccgtcgga ccggctcgga
     6961 gcggcggatc cgacggttcg caaagtgcgc cgcgcggtgc ggcatcagat gtccaggttg
     7021 cgcacttcgg gggcgttttt ctgaataaac cgccgccgcg ggtcgacctc gtcgcccatc
     7081 aggatcgtga agatctgatc ggccgtggcg gcgtcctcca gacgcacctg catcaggcga
     7141 cgcttcgccg gattcatcgt cgtttcccag agctggctgg cgctcatttc gcccagacct
     7201 ttatagcgct ggacctggac ccccttggaa ccgatctcgc gcacgcgctc gagcacctgc
     7261 ggcagcgagt cggccgccat cgcgcgccca tcgcccgccc ggacctggaa cgggtgatac
     7321 tcctcgaacg ggtttttcgg gtcgatctcg aattcctggt ggacgacgag atcggccggg
     7381 atgcccagct tctcgacgcg cgtcaggatc gcctcgacgg cgtcggcctc gggcagctcc
     7441 accagcgtat ggcgcggctt gggctcctcc agcgcggcat cggcctgatc cagcaggtcg
     7501 ggctggtcca gttcggccac cggctcctcg tcgtggccgg tctcttccag ctccgcgatc
     7561 agcgcggcca actgcttctc gctgtaggcg tagcggactt ccgcgtcgag cgagacctgg
     7621 tagcgcggca ggcgcccgcc gtcgtcgcgc atccggatgt attcgtgcag cggcagcccc
     7681 ttgcgttgca ggatgtggcg caggccctcc agcgcgatca cgtcctccag catcgcgcgc
     7741 agcgcggctt tgctcacctc caccggccgg tggccattgc cgcgggcggt gtattcgacg
     7801 acgatgtcgt cgaccgacag gttcaacagg aaccggtcgc gctgctcctc gttatccagg
     7861 tactgctcct gcttgccctt cttgacgcgg aacagcggcg gctgggcgat gtagatgtag
     7921 ccccccgcca ccagttcgcg catctggcgg aagaagaagg tcagcaacag cgtgcggatg
     7981 tgcgagccgt cgacgtcggc gtcggtcatg atgatgatct tgtgatagcg cagcttgctc
     8041 aggtcgaagt tgtccgcgcc gatgccggtg ccgagcgccg tcaccatcgt gcggatttcg
     8101 ttgttggaca ggatgcggtc gagccgggcc ttctcgacgt tgaggatctt gccgcgcagg
     8161 ggcaggattg cctggaaatg gcggtcgcgc ccttgtttgg ccgagccgcc ggccgagtcg
     8221 ccctccacca ggtagagctc gcacaggctg gggtcctttt ccgagcagtc ggcgagcttg
     8281 ccgggcaggg cggtgatgtc catcgccgac ttgcggacga tctcgcgcgc cttgcgcgcc
     8341 gccatccgcg cctcggcggc attgaccacc ttgtccacga tgcgccgcgc gacggcggga
     8401 ttttcctcca gaaattccgc cagcgactcg ttgaccatct gctcgctcca gccggcgact
     8461 tccgggttga gcaggcgccc cttgttctgc ccctcgaact gcgggttgga gatcttgatc
     8521 gagatgatcg ccgtcaaccc ctcgcgcatg tcgtcgcccg tcaggctccc cttgagcttc
     8581 ttcagcatgt cgttgcgctg agcgtagtcg ttgatcgtcc gcgtcaacgc cttgcggaag
     8641 cccgacaggt gcgtgccgcc gtcgcgcgta ttgatgttgt tggcgaaggt catcacgttt
     8701 tcgtcgtagc tgtcgttgta ctggaccgcg atctccatct ccatcgtgtc cagacgcgtc
     8761 tggccggtct cgcggtcctt gcgctcgaac tggcggctgc ggttgaagta gatcggcttg
     8821 ggatggatcg gcttcttgtt ctcattgagc tggcggacga actccatgat gccgcccttg
     8881 tagttccagc tccgctcctt gcccgtccgt tcatcgatca ggttgatcgt caggccggcg
     8941 ttgaggaacg ccagctcgcg gaaccgggtc gccagcgtct cgaaactgaa gtcgagtgtc
     9001 tcgaagacgt cgggatcggg cttgaaccgc accagtgtgc cggtcttctt cgacgcgccg
     9061 atctcgtgca gcggttcctt ggtgattccc ttctcgaacc gcatgaaatg gatcttgccg
     9121 ttgcgcttga tctcgacctc gagccaatcg gagaggaagt tcacgaccga cacgccgacg
     9181 ccgtgcagac cggccgaata cttgtaagcc tcgttgtcga acttgccgcc ggcgtgcagg
     9241 atcgtcatca cgacttcgac ggtcgacttg cccttcttcg ccgcgtgctc ctcggtcgga
     9301 atgccgcggc cgttgtcggc gatcgagact gaattatcga aatggatcgt gacgtcgatg
     9361 cggttgcaga agccggccat tgcctcgtcg accgagttgt cgatcacttc gtagacgagg
     9421 tggtgcaggc cgagcgcgcc ggtgttgctg atgtacatcg cggggcgctt gcgcaccgcg
     9481 gcgcgcccct ccagcacctg gatatccttg gcggtatatt cgcggggggc cggcgtcgcc
     9541 gccggttcga tcgtgtcagg cttcttgccc atcgtgcttg atgttccttt caacgactgc
     9601 tgcgggagaa ttggcgccct tcactcagaa aattcggctc ttggcttgcg tccatcgtcc
     9661 tcggctctcg aaaggcccgc cgcctcaaga gggacgcaac ggaccttagg gtcatgaggg
     9721 tcatgggcat cggggcttcc ggcggcgcac atacagatcg cccccgacgc tcgtccgctc
     9781 cggcgagcga tccctgtcgt ccggtcgcgg gctccgctgg cgcgtcttgt cacctgaagg
     9841 acgggacaag acggggggct tgccccccgg gaacgccggc cgagccagcg gctttggtat
     9901 cattgtgcca gatttcgcgg aaatagacag caaaaaccct ggacgaccgc gccaaaatcc
     9961 cgcgcaggcc gcgaaaatcg ctttctggcc cgcatctggg gcgggaaggc ttcgcaggaa
    10021 aaatttcggc gccccaaaat cgtcggtcgg acattcttcg aaacgttccg ctggccgcgc
    10081 gtcaacttgc ccatcaattc ctcgaatgac agaacttacg ctccaccatg ccggctgcgt
    10141 tttcgtcgta tattcgctga tgtcccttcc cgaggcaagc cggatacgac ggccgtaaac
    10201 cgtgcggccg cccgtcagga acctgctttt ccgccctccg ccagggccgt ccgcaggctc
    10261 aggatgcgct ggcgcgcgag cgtcgcctga taggtccccg gaaaccgggc ttcgatcagc
    10321 cgcagcgcct cgatggcctg cccgggccga cgattttccc gcagatgata gtccgccagc
    10381 cagtggagcc gcgtcacctg ctcgtcgatc gacatccggt tgggcaggtt cagcagccag
    10441 ctcagctcgc gcgcatagtc gtcgcgtcgg ccggcggccc acaggcgctc gcaatggatc
    10501 tccagcgcca gcgcgtcgtc cggatggcgc tccaagtggc gttccagttc ctggtcgtcc
    10561 agcgcttcga accgcgtctc gcgccgggtc ttgcgcgcga aggtcgaccc catgcggcgg
    10621 cgcgacggcg gcgttgcgcc ttcgtccaga tggttcgcca gccagcgcca cagcatcgcc
    10681 gccagggcgt gaatcgccac aatcatggcc aggccgacca gcgcctccag cgccagacgc
    10741 caaagaggat aacggccgaa cgctccggcc agcaggatca cgaggatcaa accgagcacg
    10801 gcgtccaggt agatcatgcc cgtctctcgg ggtaaggctc aggaaggcgg cggccgccaa
    10861 gccagccgca ccgggtcgaa catcgccatc gggttcagat ggaacccctc cagcgcgggg
    10921 tcgcgcaggt acggcgtcga atagatgtaa atcggcacga tcggcatctg ctcgatcagg
    10981 tacagatcgg cctggcgcag gatcgcctcg cgcgtgtcgg gatccgtttc ggcccgcgcc
    11041 tggaacagca gcgcgtcgta tgccggatcc cgccagaacg agtcgttcgt gtcgctgtcg
    11101 ctggcgaaga tttccaggaa gttgagcgga tcggggaaat cggcgaccca gccggcgcgg
    11161 gcaacgtcga agttgccctg ccgccggttc tcgatcaacg tcttgaactc ctggttttcg
    11221 accgtcatct cgatccccag ttcgcggcgc caggtctcct gaatgacctg cgcgatgagt
    11281 tgatgcgtgt cggtcatatt gtagatgtag cgcagcctgg ggaacccctg gccgcccgga
    11341 tagccggcct cggcgagcag gcgccgcgcc tcgtcgaagt cggcatcggc gaaggtcggc
    11401 gcgggaggcg cttcatacag gccggggggc gagatgaaga acgcgggctg ctcaccggcg
    11461 cgcgtgacct tctcggtgat ttcgcgccgg ttgagcgcca gcgccagcgc ccgccgcacg
    11521 cgcacgtcgc gcagcggctc cttctggcag ttcaggttga ggtagtacgt ggaaaacgcg
    11581 gtcgagaacg ccagggcctc gctgccgcgc agggaatcca gctcggcgcg cggcacccct
    11641 tcggtcgcgt cgatctcgcc gttttcgaac gcgatccgcg ccgtcgattc ctcctcgatc
    11701 atccgcagcg tcacctgctc catcttcacc gcggcggcgt tccagtattg cgggttgcgc
    11761 cgcgcgacga tgcgcgcacc gggctgatac tccgccagga cgaacggccc gcagccgacg
    11821 taagtttccg cccgccgcgc ccagtcggca ttctgttcat ccaccgcgcg gcagacgggg
    11881 aagtaggcgt gatgcgcgag cagttcgagg aaataaggcg tcggatgctc cagctcgacg
    11941 taaagctggt cgggggccag cgcccagacg cgcacggtct cggtccccag ctcggggttt
    12001 tcataatatg cccgcgcccc ggcgatataa ttgtagatct gatgggcgta ctcggcgccg
    12061 aattccggat tgagcacccg ttcggtccag gtatagagaa aatcgtcggc cgtgacccgc
    12121 tggccgttgg tccaataggc ctcgcgcagc ttgatctggt agatccggct gtccgggctg
    12181 acttcccagc tttcggccag ctcgggccgg ggcttgcctt cgtcgtcgag catgaacaac
    12241 ccgcgctgga agaagagcag ggcgcgggcc gaggcgacat cggtggcgag cgacggatcg
    12301 agcgtccgcg gatcggttcc caggttgatc acggcctgat gggtcgcctg gcgctccgcg
    12361 cgcggcgacg gcggacggca acccagcggc gcgagcgcga tcagcaggag gaacagagaa
    12421 acccggcgcg gcatcggcgg aggtccttcg gcggcaagct ggggccatcc tagaccaagc
    12481 cgggtcgcgg ggcaatcccg cccccgcaag gcggcaacgg tgccggcgct cccagcgggg
    12541 ggtcacggat atcccgtctc tgtatcccga tgaaaaggac tcgcacttga gcaggcggat
    12601 gctacgatgg cggctgagct gaagatgcgc cccctcaagg gacggggagt ttctggattg
    12661 cttgccccag gttgcgctcc gatggcgccg gtctcccacc gggggtggcg acctggattt
    12721 cgagagcgag gacgagggac gggagccgag agccgagttt tggggccggc cttcgggcgt
    12781 ggttacgagt acgaagacga gggacgagat acaggggacg agttccaaat gatagcttga
    12841 gtgaacgggg cattggagac gcgacatggc tgggaatctg cgggcgattg tcatcggttg
    12901 cgggaacatg ggggcttcgc atgcgcgggc ctatgcggcg ctggacggct ttgaactggt
    12961 cggcctggtg gcgcgcagcc cgacccggcg cggcaaactg gcggccggac tgtcgcccgc
    13021 ggctgaattt gccacgctcg aggcggcgct cgccgcgacc cggcccgatg tcgccgccgt
    13081 ctgcacctac cccgattcgc atgccgccta ctgccggcaa tgtctcgagg cgggcctgca
    13141 tgtcttcgtg gaaaagccgc tggcgatgac ggtcgaggag gccggcgagg ttgttcagat
    13201 cgcccgccgg cgcggccgca agctggtggt cggctacatc ctgcgccatc atcccgcgtg
    13261 gatccggttc gtcgagatcg cgcggacgct cggcaagccg ctggtgatgc ggatgaattt
    13321 gaatcagcag agcgtcgggg cgacgtggga cctgcacaag aacctgatgg agagcatgtc
    13381 gccgatcgtc gattgcggcg tgcattacgt cgacatgatg tgccagatga cgcgcagccg
    13441 gccggtgcgg gttcaggcga tcgccgcccg gctgaccgat gagatcgcgc cgacgatgac
    13501 caactacggc cagctccagg tcgtgttcgc ggatggatcg gtcggctggt atgaagcggg
    13561 ctggggtccg atgatcagcg aggaggccta tttcgtcaag gacgtgttcg ggccgaaggg
    13621 gagcgtcagc atcgtcaagg ggcgcgacgg cgagcaggcc catcaatccg ccaatctcga
    13681 atcgcacacc cagaccaact ggctgcgcgt ccaccacgcg gaccaggacg ccgcgcggca
    13741 attcacgcgc gaggacgagt ggatcgatac gacggacgaa ccggatcatc aggagctgtg
    13801 caatcgcgag caggcgtatc tgctgcgggc gatccgcgac gatctggacc tgaccgacca
    13861 ctgggcggat gcggtcaaca gcctgcgcat cgtgctggcg gccgatgaag cggcgcggac
    13921 cggaagaacg attgagctgt agcgccgcgc cggcgctgga tggagcggcg gaccgggacg
    13981 ggcaggaccg ggcccccaat ttcccgcggg agggatccgc cggatgaacg agaagcgcat
    14041 ctccaggcgg caactgctgc gccgaacgct gaagtgctcg gccgcggcgg ggctctttac
    14101 gatcgtgccg cgccacgtcc tgggcgggcc gggctacacg ccccccagcg agcagctgac
    14161 caagggggtc atcggcgtcg gttcgatggg ccgcacgcat ctgagctacg gcccgtcgcg
    14221 gctgctggcg atctgcgacg tcgatgaaca gcatctggag ggcgccgcgc tgcacctgag
    14281 agagaatctg ggcctggcgg aacgccaggt ccgggagatc cgacgctacc gcgacttccg
    14341 cgaactgctg gcgcagcccg acatcgacat cgtccacatc gccacgcccc cgcactggca
    14401 tgcgctgatc gcgatcgccg ccgcgcgcgc gggcaaggat atctggtgcg aaaagccgat
    14461 gacgcgcacg atcggcgaag gccgcgccgt cgtcgaggcg gtgcggcgcc acggccgcgt
    14521 cttccgcgtc aacacctggt tccggttcac cgacccgttc tacgcgttcg gctcgcgttg
    14581 ccagcccatc aagcagctcg tcgacagcgg gctgctcggc tggccgttga cggtgacggt
    14641 cggcctggct accggcttca gttggaagtt cctgtggcag ggccggacgg acctgacgcc
    14701 gcagccggtt cccgtgcatc tggattacga cctgtggctc ggcccggcgc cgttcaagcc
    14761 gtatcacccg caccgcgtgc atgaaacctt ccgcggctac tgggactacg acggcggcgg
    14821 actgggcgac atgggccagc attacctcga tccggtgcag ttcctcctcg gcaaggatga
    14881 cgaaagcccg gtgcgcgtcg aggccgatgc cccccagcag catcccgacg ccgtcggcat
    14941 tttccggcgg atcgaaatga cgtatgccga cggctgccga atcatcctgg acgggagcga
    15001 gagcgagacg cggacgccgt acattgaagg tccgaacggg cggctgtacc gcggctttga
    15061 gtgcgacgtg cccggatggc gcgaggtgct ggcgacgcgg cccgatccgg cgccgcagcg
    15121 cgacgatttc gagcaggcgg tgcgcgagcg gcgcaaattc gcgcttaacg aggccaacgc
    15181 ccatcgttcc tgcacgctgg tcaatctggc caaggcggcg gtgtatctgg ggcggccgct
    15241 gcgatttgat ccggtccgcc aggagttcat tgatgatccg gcggccaacc ggctgatcaa
    15301 tccgccaatg cgggcgccgt ggcatctgta aagcggggag gagacgcaat gaccgaaagc
    15361 atccgcgcgg ggctgctggc ggcggcctgt gcgctatatc tgacggggtg cgcgctgttt
    15421 gctccctccg gccccaggat gccggcggcc gaacgggggg cggcactcgc cgcggtggtg
    15481 gcgcgcatgc cggccgacgc cgccgcgcag gcgaacgccg acttcgccga gttggtgcgc
    15541 ctcgggccgg aggcggttcg aatgctgtgc gagcagcttg cgaccgccga gccgggggag
    15601 gccgcaggcg tgcagtacgc gctgcacgga ctggcgcagt acgtcgggcg gccgggcgcc
    15661 gggcgcgaac ggaggatgtt cgcgcgggcg gtgagcgcgg cgctgccggg aaccgccgac
    15721 ccccaggcga agaattttct gatccgtctg ctggagacga tcggcggcga cgatgcggtg
    15781 gcggcgctcg caccgctggc acatgacccg gccagcggcg acgccgccgc ccaggcactg
    15841 gccgccatcg gcacgccgcg cgcccgcgag gcgctgattg cggcccttgc cacggcgccg
    15901 cccgaccggc gcgcagccct cgcgcaggcg ctgggttata cccgggcccc cgccgccgcc
    15961 gacgcgctgc gaccgctggc gaccgacgcc gacaatgccg tgcggcgcgc cgcgctggcg
    16021 gccctcgccg accttggcga ccccgccgac gctcccctgc tggccgccgc cgtcccggac
    16081 gccgatgacc cggcggccga ctgggcggcg gacaatcaac tgcgctacgc ccggcggctg
    16141 gccgaggaag ggcatgaccg ggcggcgcgg gaaatctgcg cgcggtggct cgacccggcc
    16201 ggcgcgcgac cgacgcagtt tcgctgcgcc gcgctggcgc tcctgacgga catcgaaggc
    16261 gaagcggcca atccggcgct gctcgccgcg ctggacgacc ccgaccccca gttccgcgcg
    16321 gcggcgacct ggctcgcgct ggagctgaag ggcgacgccg tgacggaaag ctgggccgcc
    16381 cgcctggcaa ccacggccga cccggcggcg cggctcgacg ttctcgaaat gctgaccgcc
    16441 cgcggcgacc gcgccgcgct gccggccgtc ctggcgcgca tggaggatga ggagcccgcc
    16501 gtgcggctgg cggcgatcac ggccgccggc cggctgggcg gcgcggaggc ggccccagcc
    16561 ctgatcgcgg cgctgggccg gaccgatcgc gccgaggccg aggcggcgcg ggcggcgctg
    16621 atccagatcc ccggcgagga ccacctggac gccctggcgc gggcctacgc cgatgcggac
    16681 gagccgacgc gccgcggcac gctggcgatt ctggcccggc gcggtgcgac ccaacacgcc
    16741 gacctcgcgc gggcggcgct gaccgacgat gcggcgccgc tgcggctggc cgccctcgac
    16801 gcgctggccg acctcggctc ggcggacgat ctcccgcgcc tgatcgagcg cttgattcag
    16861 gcgccgacgc agtcggaggc cgcggcggcg cgccgcgcga tcgtcgcctt cgcccggcga
    16921 accggcgccg aggccgacgt ggcggaacgc ctgctccaag aactggacgg ccgcgaacag
    16981 gacgggcagc cggtcctgct cacgacgctt ggcaacctgg gatcgccggc ggggctgccg
    17041 cggctgcgcg aagcggcggg cgacgagcat cccgcggtgc gcgacgccgc cttgcgcgcg
    17101 ttgttcgact ggcccgatcc cgcctgcgcg gaggaccttc tgaccgtgat gcgcgaaaac
    17161 cccgaaccga aataccgcac gctggccgcg cgcgccctgg cgcggctggc cgccacgccg
    17221 gatctcaaag cggccgagcg gctcgatctc tgccgcaagg cgctggccgc gatcgaggac
    17281 cccgccgccg ggaaaccctt cctggcgctt ctggcggaga tcccgaccgt tgagtcgttc
    17341 cgcctggcgg cgtcgtacct ggaacacgaa gcgacgaagg ccgaggcggc actggccgtc
    17401 gccgagttgt cgccccgggt ctgccaggaa attcccggcg aagtccatgc cgcactggcg
    17461 ggcgccgccg atgccatcgc cgacgaaggc ctgcgcgaac gcgcactcac cctgatggag
    17521 aagatcggcg ccgccgacgc cgtctcgctc ttcaacggac gggatctgac cggctggatc
    17581 ggcgacaccg ccggctatgc cgtcgaacgg ggcgtgctgg tcagcaccga atcggggagg
    17641 aatttgttca ccgagaggga atacggcgat ttcgtgttga gtttcgactt ccagctctcg
    17701 ccgggagcca acaacgggct cggcgtccgc gccccgctcg agggcgacgc cgcttttgaa
    17761 ggactggaag tgcagatcct ggacgacacc gcgccgcaat acgccgagct gaagcaatat
    17821 cagtatcacg gctcggtcta cgggatcgtg ccggccgaac gcgggcatct gaaacccgtc
    17881 ggcgagtgga atcacgaagt cgtgatcgcg cgcggccgct ggatcaccgt ctatctgaat
    17941 ggccaccgga tcgtgcatgc cgatctggat caggcgagcc gggaagggac gatggatggc
    18001 cgggagcacc ccggcctggc gcgcgcgacg gggcatttgg gcctgcttgg ccatggctcg
    18061 cgcgtggcct ttcgcaacct gttcctgcgg gagctgacgc cgggcgaacc cgcggccgac
    18121 gcgtatccgc acgaaaccgc cgacctgggg atcgtgccgc cgccggcggg attcaccgcg
    18181 ctgttcaacg gccgggatct gaccaactgg caggggctgc ccgccgaacc gaacgacaac
    18241 ccgctccggc gcgcggagtt gtcgcccgcg gagctggccg ccgcgcgcca ggcggccgac
    18301 gccgaaatgc gcgcgcactg gaccgtcgcc gacggcgtgc tgcggttcga cggcaacggc
    18361 cgcagcctgt gcaccgcgcg cgattacggc gatttcgagc tgctcgtcga ctggaagatt
    18421 gagccgctcg gcgacagcgg gatttacctg cgcggcgtgc cgcaggtgca gatctgggac
    18481 agcaaccagt ggttcggcat cggctcgggc gggttataca acaatcaggg gcacccgtcg
    18541 cggccgctgg tctgcgccga tcggcccgtc ggccagtgga atacgttccg catccggatg
    18601 gagggtgatc gcgtgacggt ccatctcaat ggcgtgcgcg tggtggacga cgtggtgctg
    18661 gaaaactatt gggatcgcaa gcgtccgctc tggccgcgcg gcccgctcga gctgcaatcg
    18721 cacgcaacga agctctggtt ccgcaacata ttcattcgcg aattgcactg actcagccgc
    18781 cggttgcggc cgcgccgacc gctccccgcg cggcgtcggc gcgtgcgccg tgcaccaggt
    18841 cccaggcatt ctggaacagg atccgctcaa gcgaggcgcg atccagccgc accgactcgg
    18901 cggcttcctt gaagcctttc aaaatctgat acgccagcgg cgggtgatcc gcttcgtcca
    18961 ggtcgatgcc gtgcatccgc acgcggtatg tgctctcggt ggggaactca tagtagccgc
    19021 gcgccagcat aatcgggttg tcgaagccga aacagatccg ctcgtgaggg atcgactgca
    19081 gcgcgaggcg gtgcgtgccg ggatgcacga cggccgagaa gtcataccac cagggatggc
    19141 ggccatccag ccgctcgagc gcgcgctcca ggtagatcgg attataggcg cggcccagat
    19201 gggcgatgat caaccgcatg gcggggtagt cggcggcgag ctgaagcagg tcatcgacat
    19261 tgcgcggatc gccgaagcgc cccggccggg agatgtgcag catcacgagg gcccgatgcc
    19321 gctgggcgac gcggcacatc gccggggtca ggaattccgc gatcgcctcc tcgccggggt
    19381 gatcgccgac gacgaaatcg ggatacggct tgaagcccag aaaaccgtcg gccagcgcct
    19441 gttcgaggcg ttcctcttcg tcttccggcc gcgtcagaaa cagcacgttc agtcgctcgt
    19501 gttgccggga accttccagc agcatggcat tctgccgcgc aatgtccgat tcctgaaccg
    19561 gcatgccgaa cgccagcgcg tggacctgcc ggccgggcag cagcgccgcg tagtcctcga
    19621 agaattcctg gaccgtttgt tgcaacggca gccgcagcat ccagtagcgc gcgaggcgtt
    19681 cgggactggg cgcgcgcagg gtgcccggcg gcaccaggtg cgcgtgaagg tcgaacaggc
    19741 gccgggggag ccagtcctcg aaatgctgct cgaaataaag ctgatccgct tcgcggcggc
    19801 tgtaggtagt ggacatgcgc tgggcgcctt tcctggagtt taatggcctc tcacacaaaa
    19861 atcatctctt gttgtcctca tcctcgtccc cgtattcacc cccaaatgct cgctcgaaac
    19921 tgcggctctc gactctcgtc cctcgtcctc ggctctcgaa ggccggcgat tttgtcccct
    19981 cgttgaaccg cgagataatg agcaacataa tatcttgatc tgcatcctgt cggttctcgc
    20041 ggctcaggcc gagggaattg acaggacttc ggccggcgtg tcggccggtt cgggccgtcg
    20101 gcggcgccga tccatctcca cggcgcgcag atatcgctca tacagcgcgt caaaacgctc
    20161 ggcgcggtcg gcgcgcggct cgcagcgacg cgcgatcgga taatccggca gccgcccgtc
    20221 gggccagaag ccaagggcgc agccggccag aaacgccgcg cccagcggcg cggtgtcgct
    20281 gtgccgcatc acgctgatcg gcagccggca cgcatcggcg acccactggg gccacacccg
    20341 gccgcgcgcc cccccgccgg acatcaccag cccgccgggg tcaacgccga gcgaacgcag
    20401 acattccaga ttgcggcgcg tctcgaagac cacggccagc gcgaacccgc aggcgacgtt
    20461 ggccggcgtg tgggcgggct caagatcgag cagcgcggcg cgcgccgccg ggtcattatc
    20521 ggggcagacc gccccgaaca gatacggaat gacgatcaga tcctcggcca gattctcgtc
    20581 gcgcagcagc cagtcgtaca ccgcgcggcc ctgctgctcc gcctcaaacc gttcggcgcg
    20641 gcagaaccgg tcgatccagt ggttcagcgc gccgcagccg gtataggagg ccagcgtggc
    20701 ccacaggtcg ggccaggcat ggcagtagcg gacggcattg gactcggccg acggcgcggg
    20761 acgccgcgtc ggcgcgtaga gcacccacgc cgtgccggcg gagagaaaga cgctgccgac
    20821 cgtcggcggg ccggcgccca gaacggcgca accttgatcg tgcccgccca gcacaagctg
    20881 cacatcgtcg cgcagccccc agcgttggcg gagctcgggt cgcagcggcc ccacgatcgt
    20941 gccggcctcg ccgatctccg gcagcttgtc ttccaaatcg aaatcgcggc agagctccac
    21001 gtcccagccg cgctcccaga cgttcgcggc gctcgaaaac gacgccacgt tcaggtcgca
    21061 gacccaacgc ccggtcagcc ggtagatcag atagtccgcc acctggcaga agcggcgcgt
    21121 ccgcgcgatc agctccggcc ggtggcggcg cagccagtgg atcttcgcca ggttgcagta
    21181 ggtggcgatc ggcttgccgg tgcgggcggt ccagtcgtcg cgcaggcgct ggagttcgcc
    21241 ggtcgtggcg gcggcgcgcg tgtccatcca actgatcgcc ggggccagcg cccggcccgt
    21301 gtcgtcgcag cagatcagcg tttcaccctg gctggagagc gccagcgcgc ccacccgccg
    21361 caggtccatc tgccggccga cccgcgccag gcactcctca aggctggtcc accacagctc
    21421 gggatcctgg gtgacccgac cgccggggcc catgtcgagc ggataggtca ccgaactttc
    21481 ggccagccgg gcacccgcgg catcgagcac cagcagcttg aaattggtgg tccccacatc
    21541 gatcccgatg acggcgtcgg cggtcatggt catatgcgcg tgaattccat ctcgagcatg
    21601 tgcgccagcc gctccagaga atcgaggcgc cggccgggga tccgcgtcag gtgatgcgtt
    21661 ccgccggccc gactgtagcg atccagcagg gggccgagct cgcccttgac cgcgatcttg
    21721 aaatggggcg aatcgtaacg ttcgcccaga ttgaaatcga gcacgtcggc ctcgaacgcg
    21781 atcaggccga aacgcccgtc gccccggacg gcgacattga ccagcgtggt cggccccggc
    21841 gcgtaacgga acagcggcac cagaaagggg cgccccttgc cgaacgtgta ggggaccttg
    21901 cgcaggacag gccgctcccc ggggcgcgcc agccgataat tcccctcgcc catgtggctc
    21961 atgaagacct ggttgtgctc gaagtcggcg gtgaacatct cgcagaaacc ggccggtccg
    22021 tacagccggc cgagcaggcc gtcgagcgcc gccatcgtcg cgtcgccctc gccggcgtag
    22081 ccgcggccgg cggcgatcag atgattgacg ccgaggaacg gacaggtctc gatgcgcggg
    22141 tcttcgccga agacgccgaa atgcatggtg aaggcatcga cgccatgggc ttcgacgacg
    22201 cggcgcagcg cgatagccag ccgcgccgac cggcggtggt cctcgtccgt cacatcgggc
    22261 tcaacggcga accgctcgcg atcggcctcg atttcggcgg ccagttccgc ctcgccgatc
    22321 cgcgcctgcg cctcggccag ttcggccagc gtgagcggct cgaccgccgg accccaccgg
    22381 ctgatcagcc ccacttcgtc gaaggtgaaa tcgcccatcc cggcgaacgg ccgacccatc
    22441 aggccgactt tcatccgccg gctgagcgcc acgccgtggg ccgccttcag ccagtcggcc
    22501 agctccgcca ggtgggcctc attgcgccaa tgccccgaga ccagcccgaa agtccgcccc
    22561 gtccgccgca ggcaactgca cagatcctgc accccatgca tcccgtggtt ggccatcatc
    22621 cggtccgcat cgaagccgtc gtcgatgctg cccagatcct gcgtgttcca gatgaccagc
    22681 ggcaacggcg tccggagcag cgccggcagg ctggccagcg agagggaata agactgcagg
    22741 acgacgacca gcacgtcggc gcccgccgcc tggatccggg ccaccgtccg ctcgacctcc
    22801 tcgcgattgc agcacaggtc gtgaccgacg acctcgccgt agcggcccag gacctcgacc
    22861 cgttgagcgt gcgacgcgca gtaggtggcg agcgcctcgg ggacgacctt ccgatagaga
    22921 tcaagcgaga ggctcagcag gccgagccgc ggcggcgccg gcggctcacc ctgctcgatc
    22981 gcgtcctgct caatcgcttg cgggtcgatg acggtgctca tacaaaggtc ctcactcaga
    23041 tctcggctct cggcgcgtct tgagtccggg cagtccgaca gacaacaggg cctgacgcga
    23101 catcagggac agacggctca ctccgacaat cggcgcgtgc tctggcgttc catcagacgt
    23161 accggcgtca ggatccgttg cgcgtggaaa tcacggtgct gcaagtggtc cagcacctcg
    23221 cgaatggcca cctcgaccag ggtcgggatg gggtagttga tcgtcgtcag cgccggcatc
    23281 atgaagccct cggggtagaa atcgtcgaac ccgacgatgc tcacatcctc gggcaccttg
    23341 agcccgtggg cggcgcacgc ccgcatggcg ccgatcgcga tcgagtcatt ggccgcgaag
    23401 acggcggtgg gcggcgcggc gagccgcagc agccggccca tcgcctgctc gccgctctcg
    23461 gcgtcccagc cgccggtggt ctgcagatat tccgccgtga gcgggatgcc ggcgtcgcgc
    23521 agcgccagtt cgtaggccca gcgccgttcg gtcgcctcgt agtccccgtc gaccgagtgc
    23581 agatgcgcga tccggcgatg ccccagcgcg atcaggtgct ccaccgcccg ctgggcgcct
    23641 cccaggttgt cgggaaaaac agtgatcgcc tcctccacca ggagccgcgg ctggagcagc
    23701 acgcagggta tccgctgctc ctggagccgg cggtagagct cctggtactc cgcgtcgtag
    23761 tagacgatga tcacgccgtc gatcgagcgg tcggccgaga gcgattcaaa atagctgtcg
    23821 agccggcccg aggagaccgt gcgcacgatc agatggcaat tgagacgctg gaggatcgcc
    23881 tcggcgcccg cgatcacgct ggcgaagtac tgcgccccgg ctgtgttgtt ctgccaggcc
    23941 cggtgaaaaa tgagttcgat gttgaactcg gtggacaggg cgcgcgcccg cgagcgtttg
    24001 acgccgtact ggtggcggcg caggacctcc agcacgcgcg tgcgcgtttg cgcccgaacg
    24061 ttcttgtgat tgttgagcac ccgcgaaacc gtcgcgtacg agaccttggc ttcgcgcgca
    24121 atgtcctgca accgaacact catgagatcc actcgcaccc cctcgttggg ctcttttcgc
    24181 gaagaaccag ctcgcccgtc aatctacatc ctgggtctcg gcggaaacca gcccttttgg
    24241 tctgagagag ttgatcgtat gcgcaacgca tcttgctata catagcaaat cgtatatcat
    24301 tcagaataac cctgcgtcaa tgggaatcgt ggatgagcct cgctccaccg accggccgtt
    24361 tgacggattc ctggcgggga ttgaagatgg attccat
//
LOCUS       DAOPCO010000032        24146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2234_length_24146_cov_5.175086, whole genome shotgun sequence.
ACCESSION   DAOPCO010000032 DAOPCO010000000
VERSION     DAOPCO010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_24146_cov_5.175086"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLS90_04215"
     CDS             <1..696
                     /locus_tag="PLS90_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPK01642.1"
                     /translation="DLEWEASTDDVHVTGYEIRQDGRTIGATQDLRWKATGLEAERSY
                     QFDVAARDRDGNLSPFSEPITVATQPTSENLALNRPAYGFTMRKAGNNWNPANHYTFI
                     RQETNHSPAKAVDGDPESYVVPQPVSSPWMFQLDLERQYELDRVVVNFPAPRTCPAPF
                     LPIDLEVLVSNNIDEEIWESISTHEGVADASLTVDMKGVKARHIRVRSLANQSSAAVM
                     GIAEMEVYGSPGG"
     gene            903..2057
                     /locus_tag="PLS90_04220"
     CDS             903..2057
                     /locus_tag="PLS90_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK01643.1"
                     /translation="MSGLIKSTTRKKTKANGRSIPLYESIVRQLAEEMSSLRPGDRFS
                     TLDDLCERFQTSKITARRAISELARLGYVTAKPGVGLLVSDPREQTQTKDICFLTNGI
                     HAGGAMPLLLQGIERVARQEGYHLLLNNTEADAELNHLSVEWMLEHSVAGTVISLPHN
                     MQECDWLHEVLTRIEAHRIPAVLVDSRFIHGESKVPYVVAENYEGAMQAVEHLTQLGH
                     RRIAAINESYNSACLERKEGFRGGLLKYGADYDPKLFPDFYIHEEYERCAEAIDYFLG
                     LKAPPTAIFCNHDLIAQKCILLLQERGVRVPEDISIVGFDDLPFSEHCRPPLTTIRQP
                     LIEIGAEAAALLLHRIRVDFDYRPCKRLPTRLILRRSTAPCPGQGGSFTT"
     gene            complement(2047..2361)
                     /locus_tag="PLS90_04225"
     CDS             complement(2047..2361)
                     /locus_tag="PLS90_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01644.1"
                     /translation="MFCSWRLDDQINDVVQASGVLPAPGLVSPDEPSQDAGVPNAIVQ
                     RLDRPAIQAWHRHEVADQVDILIARIAGNGTRADASRRARPGTLQRLADHPRDGWFQL
                     TS"
     gene            2364..3152
                     /locus_tag="PLS90_04230"
     CDS             2364..3152
                     /locus_tag="PLS90_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK01645.1"
                     /translation="MRTELTHAEIESYRQNGFLRIPDFLDPDELDHWRQVIDEAVLRR
                     STRVPGGDPDDPKSFGARVFIQRQHLGSDNPDVARLVRDPRLGKLIAQLEGLPAVRFY
                     HDQALFKDPGSNATSWHLDVPFWSADTAQAVTIWVALDDSTLQNGCLHFLPGTHRTED
                     FQSTEITPDMGHFFKIRPECREIEPVAVPLPAGGCTFHNGLTAHAAGPNMTRTRRRAM
                     TISFMPEAIRFNGKPDTIAPERLATMQVGQPLDDPLFYPVIYRG"
     gene            complement(3180..3962)
                     /locus_tag="PLS90_04235"
     CDS             complement(3180..3962)
                     /locus_tag="PLS90_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPK01646.1"
                     /translation="MNGIERRRQTILDIVQQEGACTYEQLARRCNTSTMTIRRDVEAL
                     AASGAVLKTIGGILNAQAPYELYETALHDRIATNRREKQSIARKAVALIEPGQVVFLD
                     GSTTCLELASQIAQKLRGVTVITNSITACGRLAQNRDNELIAIGGQLNHDTGSFVGSF
                     AEESLLRYYPHLAFFSTKGFMVGQGTFESSIPTFRIKEIIAKRCEQVILLVDHSKFRQ
                     RSLCKVLDVSQIDTVITDGSAPTDQLKQLQDAGVEVLIAEVD"
     gene            4386..5327
                     /locus_tag="PLS90_04240"
                     /pseudo
     CDS             4386..5327
                     /locus_tag="PLS90_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
     gene            5332..6375
                     /locus_tag="PLS90_04245"
     CDS             5332..6375
                     /locus_tag="PLS90_04245"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HPK01647.1"
                     /translation="MSIAQVETRKMSIAEALRETIREEMARDERVFCLGEDIGIKGGF
                     GGAFTVTLGLSEQFGHERILDTPIAEAGIVGVAVGAALMGMRPIADVQYADFLFCAMD
                     QIVNQMAKMRYMSGGKVKVPMVLRAPCGATTRGAQHSQSPESFFNHLPGIWVACPSTA
                     YDAAGLLRTAIRCDDPVLFFEHKKLYGSKGMRAEKGALSPIGEVPQEDYTIPFGKGVI
                     RREGTDVTIVARLLMVYKALEAARLLESEGLSCEVIDPRTMVPFDTELVVESVRKTNR
                     LVIVDECNRTGGWAGDVAAEIQERAFGSLDAPILRVTAPDTPVPFAPIMERFYVPDEH
                     RVAHAVREVLKWR"
     gene            6366..7247
                     /locus_tag="PLS90_04250"
     CDS             6366..7247
                     /locus_tag="PLS90_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPK01648.1"
                     /translation="MALVRLSRLLERAAASGHAVGYFEAWDMYSMEAVVEAAEEVASP
                     VVFGFGGMMMDQPWLKRFGIEPLGHYGRAIAQAARVPAALILNEVWDYEDALCGVNAG
                     FNVLMLNTCELPFAQNLELTRKLVDEVHPRGIEVQAELGRLPNFGEDAPGTLTDPRES
                     QIFVEQTGVDFLAVSIGNVHLRTEGKARIDLDRLRAIRREVEVPLVIHGGSGFPESDI
                     RAVIAEGVSMFHVGSLMKRRYLEALLKRVGHLDEPLDYQALVGSRKTADVLVESRHAV
                     KQVVRDFIELFGSAGRA"
     gene            7252..8766
                     /locus_tag="PLS90_04255"
     CDS             7252..8766
                     /locus_tag="PLS90_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPK01649.1"
                     /translation="MSKRIVVPAAGQTTDELTLLRWWVKEGDVVRRGQVIAELETDKS
                     VMELESIHAGMVLRLHAREGEVVPVGATLMELGEPGEAVAPNGGSDAAGPTRVAGSAA
                     GAAPKRSRAGKPASRVVVPAAGQTSDELKLLRWRVALGEDVRPGQILCELETDKSVME
                     LESVHQGQVLKRVAQEGTTVMVGSTLMYVGSPGDQIADDEEVSVGGELAGNFDATASR
                     ERATDGIASGRSSGLLATPAARTLARQEALNLTEITGHGPAGCIVLANVKARASQTPG
                     GPVLIRGRREPLSRMRQTIARRLVQSVREAPHFHVAMEIDVSRLLAARVELNRQAVEA
                     DRISINDMIVKTAADTLVEHPELNCSYEEDAIHYHEAIHIGIAVRVDQGLVVPVLTDV
                     DRLTLREVSRGTKRLIADARAGKVDASVRSTFTVSNLGMYGVRAFTAIINPPEAAILA
                     IGGIDDKLVMREKAIEAVPTLIVTLSADHRVVDGDLAARFLVSLRERISRFEAV"
     gene            8806..9606
                     /locus_tag="PLS90_04260"
     CDS             8806..9606
                     /locus_tag="PLS90_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK01650.1"
                     /translation="MITASDDRRKAQWKGLREEYEREGFTIARQVIDADLVREARAHV
                     DWLMEKHPDVPPEMLKETLVSNDPFWVRLISDERLVDLAEEFIGPNIALFASSYFAKP
                     PRKGRQVLWHQDGAYWPLEPMQVVSLWLAVTSSDPENGCLRVIPRTQHLDLADLIERG
                     DVENVLGSGMDENLVEAESAVDILLQPGDVEIHHPNLIHGSNANQSDRWRIGLTIRYI
                     PTHTRITREIGVPFLLRGEAVPGVNEYLPLPKFDPQIHKDFAGSDQWR"
     gene            9678..10814
                     /locus_tag="PLS90_04265"
     CDS             9678..10814
                     /locus_tag="PLS90_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK01651.1"
                     /translation="MPTPRRFLIVRRSDFGDIAQALPLLAAIRRSVPGAFIGWLCSAP
                     VACLLRGHPMLDALHLGPACGFKGFSMHKQVALVRELRVMQYEYALDLQGDAASACWI
                     ASAGIKRRARLAPSGERLWSDRLFTNRVEPNPRLHVIERYLQLLQPVGLNAPQPEFPV
                     HLPNEARLRAEQIVGEEAGLPLIVVNPGAGSPVKRWPTRRFGELAARFLECLPCRIAV
                     AWGPGEERLAAEVLSTAGARFDDETNQKLPPTPGLHSLPRTSFPELLAIIERAALFVG
                     CDSGPTHFAAAIGTPTIAVMGSLDGRFYGPYGEEAAYVQFGIPKPPPRKADLRSWHDT
                     GSDIARLRVEDVLKMCLQMPPFRDSKSENPRAGHADRIRAPTAP"
     gene            11079..12449
                     /locus_tag="PLS90_04270"
     CDS             11079..12449
                     /locus_tag="PLS90_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01652.1"
                     /translation="MLPIRHPMPFLSWLGLAFGLALGLAPHLASPVAAQEPPPPAVDP
                     AADAPTTASITDAAPTAPPEPAEPPIDPQRLRGHHWIIDRTDLWQRSDNLLWNFHNLS
                     QFSVIHEPDDAAYPFKALFHGWPTHDQPTTGGAIFLARSPAIDAGWEVFAGVHPTSGT
                     LIWDAQMDPTLWRPVMQPGAADYAALQLSDPSLVKVGGRYLCALTAVGPNTDGIASGL
                     PGDIDGNLTCIAGAVSEDLVHWTLAPEPLLLNPADREPFEPGMLRNHYFGSYQRPALL
                     ARDDGFDLWFNYEQPNDIAMGHAINRGDFLNREDWRLVRAGADPCLSSFPNPDVVRIG
                     GLLYAFADPLRYGIHPWRGRWLTEAVSADGRNWIVLGHIKPDPDATANQVPQALVLDD
                     GDGVPRVCVFYSNQLSAPEGQPYDARMQRIRVLTRPITRLETEFYTRLLGQAAAGLAG
                     GGGTRP"
     gene            12449..13135
                     /locus_tag="PLS90_04275"
     CDS             12449..13135
                     /locus_tag="PLS90_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01653.1"
                     /translation="MEFRVRPDRERGSGMEILRLAGIPFYAEFSFFIFAALLVFLFLT
                     WDWTIQQAGLQVIIVLISLIAHEGGHAVMARLMGCRQVSVSLIMFGGQTYHEPTTNGR
                     NLLIVLAGPALTLVLMLFGFGLLFVPAVHASDAGRFVCGSIGIMNLIWFIFNMLPIFP
                     MDGGMTILYTLAFFMREHRAMFFVSLVSMAACILAGLALYLSGLQVTMAYIFLILFFL
                     QNMAVYKTYR"
     gene            complement(13139..14365)
                     /locus_tag="PLS90_04280"
     CDS             complement(13139..14365)
                     /locus_tag="PLS90_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01654.1"
                     /translation="MIDCELLLRRDARDYRWTPVAGPIAPAHYDAAIADLSARGRSIP
                     GVVALYDFGPIRYPGLSDIDLLFVVRDDFAAFDALRALLHASVAPRYHNVLLHNPYFV
                     RARELPLYFKFAPFFELNRRWGTGDADLPARLSNDDPLDLTLFLLDIVIDAYPWEFVR
                     YLTLERIDERELMSRVKGLSYCFALLDRIGARQAEGFRARAAAFSEAIAGLRERYFDL
                     APVERQARLLTLTQRAVGLSQELIGVLAEHLRAAWGLRLAGDLILSCTRVAALYRGAA
                     PAEPRVGAVRLRRTDYYVQQPVELGYVVWEYMQRAGPVTNRFRRRSIDRLRVEQAPAP
                     AIREAIGRRADLRNDHQAWLDRMALGHPGFMTFGYRTRQRGGGGAARRIAGRVLDAAR
                     EALLRRRLIALRDGGE"
     gene            complement(14436..16109)
                     /gene="recN"
                     /locus_tag="PLS90_04285"
     CDS             complement(14436..16109)
                     /gene="recN"
                     /locus_tag="PLS90_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPK01655.1"
                     /translation="MLRSLHIENLATIEALTIEFGEGLNALTGETGAGKSIVVGGLGL
                     ALGDRAAAEAIRAGARVAVVEACFALPSPPAGLTRLLTDELELEWTPAEPLTLRRELS
                     AAGRNRCFVGGQMVNVADLRRIGELLVDFHGQHEHQSLLHASAARDALDAHAGHAEVV
                     EAYRRAWREVVELRRRRDELDQAAREFEQRLDYLEFQLGEFDKVNPRAGELLELEHEE
                     HRLARADELTRSAAEAYALLYEGSDDQPSVLGQLREVERRIGALAEVEPPFAERLATL
                     AEQKAALEDLAYALRDYADQVEADPARLDEVIARSEAIRRLTRKHGGTEESLFAAVEG
                     MRRERERMERDDAERREIGARVAEAERRLRAAGEKLRAGRKAAAERFAREVGALLKRL
                     HMDKARFEVLLEELEAPGGDGMDRVELLLAANPGLPPAPLRKIASGGELSRVMLAIKT
                     ALARRDAIATLVFDEIDAGISGETAVRVGAVMEKLGESHQVLCITHHAAIAARAARHL
                     AVSKSARRRETFTEVTELTGSARLDELARLMGGDAATEAGRELARQLMG"
     gene            16418..17884
                     /locus_tag="PLS90_04290"
     CDS             16418..17884
                     /locus_tag="PLS90_04290"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HPK01656.1"
                     /translation="MGKRTRDYLALDLGAESGRAIVGRFDGRRLRLTELYRFTHDSLR
                     FNGTLRWHVLGMAAHLRLALAAAAAASRGNLRSLGVDSWGVDYVLLDRNDDLLGYPWQ
                     YRDARTDPALPAVFRQIPPLELFRLTGIQPLWFNTLFQLYAEVMQRTGAIEAARTLLF
                     IPDFLTWTLGGEKACEYTIASTSQMLDARRRNWARGLLRRLNLPTALLPPLVAPGALS
                     GRIRPEVARETGAPRSLRIVAVGRHDTASAVAAVPAADAGDWAFLSSGTWSLVGTEES
                     RPALTEQALAGNLTNEGGLGGAYLLLANIMGLWLVQELRRAWTDRRGRAPDYAELTAA
                     AAAAPSGGPRVDPDWPPFGRPDAMPEKIVRYCRKTRQTPPRTPGEFVRCALDSLAAAY
                     RRALDNLEAVRGRPIHRLHIVGGGSRNELLNQLAADATGREVLAGPVEATAIGNILAQ
                     LLADREIHSIAEGRAIVQASFRPKRFLPRPNRPTSSSP"
     gene            complement(17997..19394)
                     /locus_tag="PLS90_04295"
     CDS             complement(17997..19394)
                     /locus_tag="PLS90_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK01657.1"
                     /translation="MAASDKPGRVLVVDDEWSIRDVLSNILQSEGFEVETAQDGDEAI
                     ALLEDKLYDLVITDLKMPRVHGMEVLRHLSKLNVHTLGIVATGYGSIESAVEALRLGA
                     FDYITKPFHLDEIKIVVHKAREFQALQTENRTLRRELKRSGKFENIIGNSAKIKSMIN
                     LIHTVADSDSTILILGESGTGKELVARAVHYNSPRADQPLIPVNCGAIPEDLLESELF
                     GHVKGAFTGATMNRVGRFQLADGGTIFLDEIGDMSPKLQVKLLRVLQEQAFEPVGATE
                     TVRVNVRIITATNRDLEADVADGRFREDLFYRLNVIPIHIPPLRERAEDIPILIDHFV
                     ERFNKLKGRRVKGFRRDAIEALMAYPWPGNVRELENLVERMVILNPDGEITEETLPER
                     FTGHKPRPLPMPSEGLEIPDEGIDFNALVDQFETQLIERALEKAGGIKNKAAALLQIK
                     RTTLVEKMKKKGMLE"
     gene            complement(19681..20511)
                     /locus_tag="PLS90_04300"
     CDS             complement(19681..20511)
                     /locus_tag="PLS90_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HPK01658.1"
                     /translation="MAETRKEILNRFRKKIAEGRPIIGGGAGTGISAKWEEAGGIDLI
                     VIYNSGRFRMAGHGSLAGLLSYGDANAIVMEMAREVLPVVKQTPVLAGVCGTDPMRQM
                     PQFLSEVKRIGFAGVQNFPTVGLIDGVFRANLEETNMGYDKEVEMIRQAHELDLLTTP
                     YVFDTTDAEKMTRAGADILVAHMGLTTSGSIGARTAKTLDECVTLIDEIVRTARAIRD
                     DVIVLCHGGPIAEPEDARYVLERCQHVDGFYGASSMERLPVERAIKQQTEEFTKIRRK
                     "
     gene            complement(20659..21747)
                     /locus_tag="PLS90_04305"
     CDS             complement(20659..21747)
                     /locus_tag="PLS90_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01659.1"
                     /translation="MAYRVIQIGTGGFGACWCEWFLPANVREGLIEVVAAVDINPDNL
                     AHARKHLNLPAERCYTDVRRALDENPADFCTIVVPPAHHETMVDHALAHDLHILSEKP
                     IADTLEASCRIVEKVKRAGKKMAVTMSHRFDQDKTTLREELRSGRHGELDYIVSRFTC
                     PCRAFASWGKFRHEIPDPLMVEGAVHHLDLLADMGGALCEEIYAQTWNPRWGEYAGDS
                     QGLVTMRLANGKRIFYEGAKTNTAGLNCWGNEYIRAECEKATLILDTRRLEKFALPAG
                     KSGVSAREGHGETIPLLEQPKWQNAWLVEQFVKWLDGGPAMETNVEANLQSVALIFAA
                     IESSRTGMPVKVQELLRRVWDQVKREMA"
     gene            complement(21763..22986)
                     /locus_tag="PLS90_04310"
     CDS             complement(21763..22986)
                     /locus_tag="PLS90_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HPK01660.1"
                     /translation="MGKTIALLGALDTKGAEYGYVRDLIHARGHKTFIIDVGVMGQPA
                     IAADLTREEVARAGGGDVAALAGKGDRGEAVALMSRGAAAAASRAHAEGKFDAILAMG
                     GTGGTSVACNAMRALPLGVPKVMVSTVAGTDVSGYVGVKDIVMIPSIVDVAGINRISR
                     EVFARAAGAVCGMVEIEIPAGQDKPLVTASMFGNTTAAVERARQILEENGYEVLVFHC
                     TGTGGRTMESLIEAGLIAGSLDITTTEWADELVGGVFAAGPTRLEAAARRGVPAIVVP
                     GCLDMVNFGAPETVPEKFKGRTFYQHNPNVTLMRTNVEENRQLGRILAEKINLSTAPV
                     SVLIPLRGISVISAEGKAFYSPEADKALFDELKANLKPGVPVIELDCNINDAAFAEAC
                     AQELLKNLKADAAAR"
     gene            complement(23096..23881)
                     /locus_tag="PLS90_04315"
     CDS             complement(23096..23881)
                     /locus_tag="PLS90_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK01661.1"
                     /translation="MMGKRVGVRRSGVAVAGVIGLVIGVSLTGGCSLMNERLNTAPGL
                     HHASARVTLRKELAFRYLAYLPDGYEAEAERRWPLVLFLHGAGERGDDLNKVALHGPP
                     RRIQEGERFPFILVAPQCPADAWWEPETLIPFVDYVESAYRVDRDRIYVTGLSMGGFG
                     TWGLALAQPERFAAIAPVCGGGLAFYTRRIPHLPVWVFHGARDSVVPLSESERMVEAL
                     RRYGGNVKFTVYPDADHDSWTETYANPELYAWLLAQRRGAPAR"
BASE COUNT         3930 a         8685 c         7256 g         4275 t
ORIGIN      
        1 gacctggaat gggaggcctc gaccgacgat gtccacgtca ccggctacga aatccggcag
       61 gatgggcgca cgatcggggc cacacaggat ctccgctgga aggcgaccgg cctggaggcc
      121 gaacgctcct atcagtttga tgtggccgcg cgtgaccgcg acggcaatct gtctccattc
      181 tcggaaccga tcaccgtggc gacacagcca acgtccgaga atctcgcgct caatcgtccg
      241 gcctatggct ttacgatgag gaaggcgggc aacaattgga atccagccaa tcactatacc
      301 ttcatccggc aggaaaccaa tcacagtcca gcgaaggcgg tcgacggcga tcccgagagt
      361 tacgtcgtgc cccagccggt atccagtccg tggatgttcc agctcgacct ggagcggcag
      421 tatgagctgg atcgcgtcgt ggtgaacttc cccgcgcccc gaacctgtcc cgctcccttt
      481 cttccgatcg acctcgaagt cctggtttcg aacaacattg atgaggagat ctgggaatcg
      541 atcagcacgc atgagggggt cgcggatgcg tcgctgaccg tcgatatgaa aggggtgaag
      601 gcccgtcaca tccgcgtccg ttcactggcc aatcaatcga gtgcggccgt catgggcatc
      661 gcggaaatgg aggtgtacgg cagtcccgga gggtaaggcc tcgaacaggc tggaagccgc
      721 cgctggagag gcggcgggca gggccggacg gccgaataga ttctcctgtt tcttgcagcg
      781 ggcttgtgta taattaattc ataaggggac gttcgcgcgc tcgcgaactg gttcagacga
      841 ctaacttgcc cgtccggatc ggttgaccgc gcggagcctc gattcgcgtt catggagcca
      901 agatgtccgg tttgatcaaa agcacaacca gaaagaagac gaaggccaat ggccggagca
      961 ttccgctgta cgagagcatc gtgcggcagc tggccgagga aatgtccagc ctgcggccgg
     1021 gggatcggtt ctccacgctg gatgaccttt gcgagcgttt ccagacgagc aagatcaccg
     1081 cgcggcgggc gatctcggag ttggcgcggc tgggctacgt cacggcgaag cccggggtgg
     1141 gtctgctggt ttccgacccg cgcgagcaga cccagacgaa ggatatctgt tttctgacca
     1201 atggcattca cgccggcggc gccatgcccc tgttgctgca ggggatcgaa cgagtcgccc
     1261 ggcaagaggg ctatcacctg ctgttgaaca ataccgaggc cgatgccgaa ctgaatcacc
     1321 tgagtgtgga atggatgctg gagcattcgg tcgccgggac ggtcatcagt ctgccgcaca
     1381 acatgcagga gtgcgattgg ctgcacgagg tgttgacgcg gatcgaagcg catcggattc
     1441 ccgcggtcct cgtcgactcg cgcttcatcc acggggaaag caaggtgcca tatgtggtcg
     1501 cggagaatta cgagggcgcg atgcaggccg tggagcacct gacacagttg ggtcaccggc
     1561 ggatcgccgc gatcaatgag agctacaatt ccgcctgcct cgaacgcaag gaaggttttc
     1621 gcggcggttt gctcaagtac ggcgcggact acgatccgaa gctgttcccg gacttttata
     1681 ttcatgagga gtatgaacgc tgcgccgagg cgattgatta ttttctcggt ctgaaagctc
     1741 cgcccaccgc gattttctgc aatcacgacc tcatcgcgca gaaatgcatt ctgttgctgc
     1801 aggaacgggg agtgcgcgtt ccggaggaca tttcgattgt cggcttcgat gatctcccct
     1861 tcagcgaaca ctgtcgcccg ccgttgacga ccatccgcca gccgctgatc gaaattgggg
     1921 cggaagccgc ggccttgttg ctccatcgca tccgggttga cttcgattat cggccgtgca
     1981 agcgcctgcc gacgcgtctg attctccggc gctccaccgc cccttgtccg ggacagggcg
     2041 gatcgttcac gacgtgagtt gaaaccaccc gtcccgggga tggtcggcca gccgctgcag
     2101 cgtgccggga cgagcccgac gggaggcgtc ggcgcgtgtc ccgttgccgg cgatccgcgc
     2161 gatgaggata tcgacttgat ccgcaacttc atgccggtgc cacgcctgga tcgccggccg
     2221 gtccaggcgc tgcacgatcg cattggggac gccggcatct tgcgagggtt cgtctgggga
     2281 gaccaggccg ggtgctggca gcacgccgct tgcctgtacg acatcattga tctgatcatc
     2341 aaggcgccag gagcagaaca tcgatgcgga ccgagctgac ccatgcggag atcgaatcct
     2401 atcgccagaa cggcttcctg cgcatcccgg attttctcga tccggatgaa ctggatcact
     2461 ggcgacaggt catcgacgag gcggtactgc gacgcagcac tcgggtgccc gggggcgatc
     2521 ccgacgatcc gaaatcattt ggggcacggg ttttcattca acggcagcac ctcgggtcgg
     2581 acaatccgga cgtggcccgg ctggtgcgcg atccgcgtct cggcaagctg attgcgcagt
     2641 tggaaggtct gcccgccgtt cgcttctatc atgatcaggc cttgttcaaa gatccgggga
     2701 gcaatgcgac aagctggcat ctggacgtgc cattctggtc ggccgatacg gcgcaggccg
     2761 tgacgatctg ggtggcgctg gacgactcga cgctccagaa cggttgcctc cattttctgc
     2821 cgggaacgca ccggacggag gatttccaat cgacggaaat cacgcccgac atggggcact
     2881 ttttcaagat tcggcccgaa tgccgggaga tcgaaccggt cgccgtgccg ctgcccgccg
     2941 gcggctgcac cttccataac ggcctgaccg cccatgccgc cgggccgaac atgacccgca
     3001 cgcggcgccg cgcgatgacg atcagcttca tgcccgaggc catccggttt aacggcaagc
     3061 ccgataccat tgcgcccgaa cggctcgcca ccatgcaggt ggggcaaccg ctggatgatc
     3121 cgctgttcta tccggtgatc tatcggggct gagaagttca acaggcacga gcgcgggcgt
     3181 caatcgactt cggcgatcag cacctccacg ccggcatcct gcagttgctt caattggtcg
     3241 gttggagcgc tgccgtcggt gatgacggta tcgatctggg agacgtccag gactttgcag
     3301 agcgagcgct ggcggaattt ggagtgatcg acgagcagga tcacctgctc gcaacgtttg
     3361 gcaatgatct ccttgattct gaaggtcggg atcgacgact cgaacgtgcc ttgcccgacc
     3421 atgaagccct tggttgaaaa gaaggccagg tggggatagt agcgcagcag ggattcctcg
     3481 gcgaatgaac cgacaaagga cccggtgtcg tggttcagct gcccgccgat cgcgatcaat
     3541 tcgttgtcgc gattctgcgc cagccggccg caggccgtga tggaattggt aatgacggtg
     3601 acgccgcgga gtttctgcgc gatctgggaa gcaagttcca ggcatgtggt gctgccatcg
     3661 aggaagacaa cctgacccgg ttcaatcaac gcgacggcct tgcgcgcaat gctctgcttt
     3721 tccctgcggt tggtggcaat tcgatcgtgc aacgccgtct cgtagagctc gtagggcgct
     3781 tgggcattca ggattccgcc gatagtcttg agcacggcgc cgctggccgc aagcgcctcc
     3841 acgtcgcgcc ggatcgtcat ggtggaggta ttgcagcgac gcgccagttg ttcgtacgtg
     3901 caggcccctt cctgctgcac gatgtcaagg atggtctggc gtcgacgttc gatcccgttc
     3961 atctgcgcct gcttctccag ccgccgaccg gctcctgggg tttccgatcc atggaagcgg
     4021 gcaatccgaa gggggaggcc ccgctcacag cggcatggta atccatcgtc ctccagctta
     4081 gctatgaaac tttttaacat gttccggccc cgcgagtgga ccgggaactt ccatgtaata
     4141 ctaggaataa ggcggcaatc gggccgggca gcgcttacgc agcgatgaga aagattctca
     4201 ttgataatgt gaatcattat cactatcttc aagtcatgtc gtcgcgacct gttggccgga
     4261 cgagccgcct tttaaggctg ccctcccggt gaatccctga aaggccagga ccagccatgg
     4321 acaacgggct gcccaacgca gggcccaagc gcgtaacgga ataccccccc gacctgctgc
     4381 gccggatgta tctgcagatg cagcgcgcgc gccagttcga gctgcgcgtc aaccagatgt
     4441 tcctcgaggg cgccctgccc ggcacgctgc acctttacga agggtaggaa gcgtgcgcgg
     4501 tgggggtgtg cgaaaacctg agcgacgagg actggatcac cagcacgcat cggccgcatg
     4561 gccacgccat cgccaagggg gtctcgctcg acgcgatgat ggcggagctg ttcgcgcgca
     4621 cgaccggctg cgtcgggggt tacggcggct cgatgcatgt gggcgatccc gccgtcggcg
     4681 cgctgccggc gatcgccatc gtcggcggca atgcgccgat tgccgccggg atggcgctgg
     4741 cgctgaagat gcgtaaatcg cgccatgtcg tctgctgctt ccacggcgac ggcgcggtga
     4801 acgagggtgc ctatcacgaa ggggtcaaca tggcggcgat ctgggatttg cccgtggttt
     4861 tcatctgcga gaacaatcag tacggcgcct cgacgccggt ggcgcaggtg acccggttgg
     4921 agcgtctggc ggaccgtgcc cggggctacg gcatccccgg gatcaccatc gatgggatgg
     4981 atgtgctggc ggtttatgag gccgtcggca tggccgtggc gcgcgcgcgc cggggcgagg
     5041 ggccgatgat cgtggaatgc gagacctatc gctactgcgg ccactcgcgc agcgacacgc
     5101 gcggctatcg cgcgcgcgag gaggaagcgc gttggcgcga gcgggacccg atcctcaatc
     5161 tcggccgccg gctcgtggag gccggcttgt ttaccgacca ggatctggcc gactgggatc
     5221 agagcgtcca ggacgagttg gatcgcgccg tccagttcgc catggccagc cccgagcccg
     5281 acccgcgcga ctgcctgagc catgtccatg tggagcaagg atggtgatgc catgagtatc
     5341 gcgcaagttg aaacccggaa aatgtcgatt gccgaagcgc tgcgcgagac gattcgcgag
     5401 gagatggcgc gcgacgagcg cgtcttctgc ctcggcgagg acatcggcat caagggggga
     5461 ttcggcgggg ccttcaccgt gacgctgggc ctgtcggagc aatttggcca cgagcggatt
     5521 cttgacacgc cgattgcaga ggcgggcatc gtcggcgtgg ctgtcggcgc ggcgctgatg
     5581 gggatgcgtc cgatcgccga tgtgcaatac gccgacttcc tcttctgcgc gatggaccag
     5641 atcgtcaacc agatggcgaa gatgcgttac atgtcggggg gcaaggtcaa ggtcccgatg
     5701 gtgctgcgcg cgccctgcgg cgcgactacc cgcggcgcgc aacacagcca aagcccggaa
     5761 tccttcttca accatctccc ggggatctgg gtcgcctgcc cgtcgacggc ctacgatgcg
     5821 gccgggctgc tgcggaccgc gatccgctgc gatgacccgg tgctgttctt cgagcacaag
     5881 aagctctatg gcagcaaggg gatgcgcgcg gaaaagggcg ccctctcgcc gatcggcgag
     5941 gtgccccagg aagattacac gattcccttc ggcaaggggg tcatccggcg cgaggggacg
     6001 gacgtcacga tcgtcgccag gctgttgatg gtttacaagg cgttggaagc ggcccggctg
     6061 ctggagagcg agggcctctc gtgcgaggtg atcgatccga ggacgatggt cccgttcgat
     6121 accgaactgg tggtcgagtc ggtccgcaag acgaatcggc tggtcattgt ggatgagtgc
     6181 aaccgcaccg gcggatgggc gggcgatgtt gccgccgaga tccaggaacg cgcgttcggc
     6241 tcgctcgacg cgccgatctt gcgcgtgacc gctcccgaca cgcccgtgcc gtttgctccg
     6301 atcatggagc ggttctacgt ccccgatgag caccgcgtgg cccatgccgt ccgggaggta
     6361 ttgaaatggc gttagtgaga ctgtcgcggc tgctggagcg ggccgccgcc agcggccatg
     6421 cggtcgggta tttcgaggcc tgggatatgt attccatgga agcggtggtc gaggcggccg
     6481 aggaagtggc gtcgccggtg gtgttcggct tcggcggcat gatgatggac cagccgtggc
     6541 tcaagcgctt cgggatcgag ccgctcggcc actacggtcg ggcgatcgcg caggccgcgc
     6601 gcgtgccggc cgcgctcatt ctcaatgaag tctgggatta cgaggacgcg ctttgcggcg
     6661 tgaatgcggg attcaacgtc ctgatgctga acacctgcga gctgcccttc gcgcaaaacc
     6721 tcgagttgac gcgaaagctc gtggacgagg ttcatccccg cggaatcgag gtccaggccg
     6781 aactgggccg tctgccgaac ttcggcgagg atgcgccggg cacgctcacc gacccgcgcg
     6841 agtcgcaaat cttcgtcgaa cagaccggcg tggatttcct cgcggtttcc atcggcaatg
     6901 tgcacctgcg caccgaggga aaggcccgta tcgatcttga ccgactgcgt gcgatccggc
     6961 gggaagtgga ggtccccctg gtcatccatg gcggcagcgg ctttccggaa agtgacatcc
     7021 gggcggtgat cgccgaaggc gtctcgatgt ttcatgtcgg ctccctgatg aagcgccgct
     7081 atcttgaggc gctgttgaag cgcgtcggcc atctggacga gccgctggac taccaggcgc
     7141 tggtcggcag ccgcaagacg gccgatgtgc tggtcgagtc aaggcatgcg gtcaagcagg
     7201 ttgtccggga tttcattgaa ctgttcggct cggccgggag ggcatagcca gatgtcgaag
     7261 cgcatcgtcg ttcccgcggc cgggcagacc accgatgaac tgacgctcct gcgctggtgg
     7321 gtgaaggaag gcgatgtcgt ccggcgcggt caggtgatcg ccgagctcga gacggacaag
     7381 agcgtgatgg agctggaatc gattcacgcc ggaatggtcc tgcggctgca cgcgcgggaa
     7441 ggggaggttg tgccggtcgg cgcaacgttg atggaactgg gcgagccggg cgaagccgtg
     7501 gcacccaacg gcgggtcgga cgcagccggt ccaacccgtg tcgccggatc cgcggcgggc
     7561 gccgctccga agcggagcag ggcgggcaaa ccggcctcgc gcgttgttgt tccggccgcg
     7621 gggcagacct ctgacgaact gaagctgctg cgctggcggg tcgcgttggg cgaggatgtc
     7681 cgcccgggcc agattctctg cgagctggag acggacaaga gcgtcatgga gctggaatcg
     7741 gtgcatcagg gacaggtcct caagcgggtt gcgcaagaag gcacgaccgt gatggtgggg
     7801 tcgactctga tgtatgtcgg gtctcccggc gatcagatcg ccgacgacga ggaggtttcc
     7861 gtgggcgggg agctcgcggg caacttcgac gccacggcct cccgtgagcg cgcgaccgac
     7921 gggatagcca gcgggcgctc gtcgggcctg ctggccacgc cggcggcgcg cacgctcgcg
     7981 aggcaggaag ctctcaacct gactgagatc accggccatg ggccggctgg ctgcatcgtg
     8041 ctggcgaacg tcaaggcccg ggcgagccag accccaggag ggccggtgct gattcgcggg
     8101 cggcgcgagc cgctcagccg catgcggcag acgatcgcca gacggctggt ccaaagcgtg
     8161 cgggaagccc ctcacttcca cgttgccatg gaaatcgatg tgtcgcgcct gctcgcggcc
     8221 cgcgtggagc tgaaccggca agccgtggaa gcggatcgca tcagcatcaa cgacatgatc
     8281 gtgaagaccg ccgcggacac gctggttgag catcccgagc tgaattgcag ctacgaagag
     8341 gacgcaatcc attaccacga ggcgatccat atcgggatcg ccgttcgtgt cgaccaggga
     8401 ttggtcgtgc ccgtgctgac cgacgtggac cggttgacgc tgagggaagt cagccgcggg
     8461 acaaagcgac tgatcgccga cgcgcgcgcc ggcaaggtcg atgcgagcgt ccgatccacc
     8521 ttcacggtgt ccaacctggg tatgtatggc gtgcgggcgt tcacggcgat tatcaatccg
     8581 ccggaagccg ccattctggc gatcggcggc atcgacgaca agctggtgat gcgggagaag
     8641 gcgatcgagg ccgtgcccac cctgattgtc acgctgtcag cggaccatcg cgttgtggac
     8701 ggcgatctcg cggcccggtt cctcgtttcg ctgcgagagc gcatttcccg gtttgaagcg
     8761 gtctgatcag aacaatgaac ctgttagccg gaaggagaat cgcccatgat caccgccagc
     8821 gatgaccgcc gcaaggctca atggaagggg ctgcgcgagg aatatgagcg cgagggcttt
     8881 acgatcgcgc gccaggtgat cgatgccgac ctggttcggg aagcgcgtgc gcacgtcgat
     8941 tggctcatgg aaaagcaccc ggatgttccg cccgaaatgc tgaaggaaac gctggtgagc
     9001 aatgacccat tctgggtgcg cctgatcagc gatgaacgcc tcgttgacct ggcggaggag
     9061 tttatcggtc cgaatatcgc cctgttcgct tcaagctatt tcgccaagcc gccgaggaag
     9121 gggcgccagg tcctgtggca tcaggacggc gcctactggc cgctcgagcc gatgcaggtg
     9181 gtttcgctct ggcttgcggt cacttcctct gacccggaga atggctgcct gcgcgtcatc
     9241 ccccggacgc agcatctgga tctggcggat ctgatcgaac ggggtgatgt cgaaaacgtg
     9301 ctcggttcag gcatggacga gaatctcgtt gaggccgaat cagcagtcga tatcctgctg
     9361 caacccggcg atgtcgagat ccatcatccc aacctgatcc atggctcgaa tgccaatcag
     9421 tccgatcgct ggcggattgg gctgacgatt cgttacatcc cgacccatac ccggatcacc
     9481 agggagattg gcgtgccgtt tctcctgcgc ggcgaagccg tgcccggggt caatgaatat
     9541 ctgccgcttc cgaaatttga cccgcaaatt cacaaggatt tcgccggcag cgaccagtgg
     9601 cgatagcccc gcctcgcgtg gaggtccccc gcgcaatcct tcgcgcctga acgtattcga
     9661 atggggagaa accccgggtg ccaacgccgc gccggttcct cattgtccgt cgctcggact
     9721 tcggcgacat tgcgcaagca ttgcccctgc tggccgcgat ccgccgctcc gtgcccggcg
     9781 ctttcatcgg ctggctttgt tctgctcccg tggcatgtct gctgcgcggc caccccatgc
     9841 tcgacgcgct gcatctcggg ccggcctgcg gattcaaggg cttttccatg cacaagcaag
     9901 ttgcattggt ccgcgaattg cgggtgatgc aatacgaata tgcattggat ttgcagggcg
     9961 acgccgcgag tgcctgctgg atcgcgtccg ccggcatcaa gcgccgagcg cgactggccc
    10021 cgagcgggga gcggctctgg agtgaccgcc tgttcacgaa tcgcgtggaa ccgaatccgc
    10081 gtctgcacgt gatcgaacgc tacctgcagt tgctccagcc cgtggggctg aacgcgcccc
    10141 agcccgaatt tccggttcac ctccccaacg aagcgcgtct ccgggccgag cagatcgttg
    10201 gggaagaagc tggcctcccc ctgatcgtcg tcaaccccgg ggcgggatct cccgtcaaac
    10261 gctggcccac acggcgtttc ggcgaactcg ccgcccgatt tctcgaatgc ctgccctgcc
    10321 ggatcgccgt ggcttggggc ccgggcgagg agcggcttgc ggccgaggtt ctttccacgg
    10381 ccggcgcccg gtttgacgac gaaacgaatc aaaagttgcc gccgacgccc ggcctccatt
    10441 cgcttcccag gacgagtttt ccggaattgc tggcgatcat cgagcgggcc gcgctgttcg
    10501 tcggctgtga ttccgggcca acgcatttcg cggcggccat cggcaccccg acaatcgcgg
    10561 tgatgggttc cctggatggc cgcttttatg ggccgtatgg ggaggaggcc gcctacgtgc
    10621 aatttggaat acccaagccg cctccccgaa aggccgatct cagatcatgg catgacaccg
    10681 gttcggatat cgcgaggctg cgcgtggaag acgtcttgaa aatgtgtctg cagatgcctc
    10741 cgtttcggga ttcgaagagc gagaacccgc gggccggcca tgcggatcgg atccgcgcgc
    10801 caaccgctcc ctgaccgaaa gttccaggtt ctcccccagg ctcttccgcc agcccttcac
    10861 gagcctgggc gctcctttca actgaccagc tacacttcac tggtcctccg tccacgatgg
    10921 caaccaccgt ctgggaccgc cggcatcctt gtcggcaatc catgcgcatg ctccctcggc
    10981 cgtaacttct tcgccgtgca ttgacatcct cctttccccg ccggttgcag tgtgaccgcg
    11041 cgtcgactag aattgcgccg cggagggaag agcctgccat gctcccgatt cgacatccta
    11101 tgccattcct gtcgtggctc ggccttgcgt tcggtctcgc gctcggcctt gccccgcacc
    11161 tcgcgtcgcc ggttgccgcg caggaaccac cccctcccgc ggtcgacccc gccgcggacg
    11221 ctccgaccac cgcctccatc acggacgccg cgccgaccgc gccgcccgaa cccgccgaac
    11281 cccccatcga cccgcagcgc ctgcgcggcc accactggat catcgaccgc accgacctgt
    11341 ggcagcgcag cgacaatctg ctgtggaact tccacaacct ctcgcagttc agcgtgatcc
    11401 acgaacccga cgacgccgcc tatccgttca aggcgctgtt ccatggctgg cccacgcacg
    11461 atcagccgac gaccggcggc gcgatctttc tggcgcgcag ccccgcgatc gacgccggct
    11521 gggaggtctt cgccggcgtg caccccacca gcggcaccct gatctgggac gcgcagatgg
    11581 acccgacgct ctggcggccc gtgatgcagc ccggcgccgc cgactacgcc gccctgcagt
    11641 tgagcgaccc ctcgctggtc aaggtcggcg gccgctatct ctgcgcgctg accgccgtcg
    11701 gtcccaacac cgacggcatc gcctcgggcc tgcccggcga tatcgacggc aacctgacct
    11761 gcatcgccgg cgccgtctcc gaggacctcg tccactggac cctcgccccc gagccgctcc
    11821 tgctcaaccc ggccgaccgc gaacccttcg agcccggcat gctgcgcaac cattacttcg
    11881 gctcgtatca gcgccccgcc ctgctcgccc gcgacgacgg cttcgacctg tggttcaact
    11941 acgagcagcc caacgacatc gccatgggcc acgcgatcaa ccgcggcgac ttcctcaacc
    12001 gcgaggactg gcgcctggtg cgcgccggcg ccgacccctg cctgagcagt ttccccaacc
    12061 ccgacgttgt ccgcatcggc ggcctgctct acgccttcgc cgatccgctg cgctacggca
    12121 tccatccctg gcgcgggcgc tggctcaccg aggccgtctc cgccgacggc cgcaactgga
    12181 tcgtcctcgg gcacatcaag cccgaccccg acgcgaccgc caaccaggtg ccccaggcgc
    12241 tcgtgctcga cgacggcgac ggcgtccccc gcgtctgcgt cttctactcc aaccagctca
    12301 gcgcgcccga gggccagccc tacgacgccc ggatgcagcg catccgcgtc ctgacccggc
    12361 cgatcacacg attggagacc gagttctaca cgcgcctgct cggccaggcc gccgcggggc
    12421 ttgccggcgg gggagggacc cgcccctgat ggaatttcgc gtccgtccag accgcgagcg
    12481 cggcagcggc atggaaatcc tgcgcctcgc cggaattccg ttctacgccg agttctcctt
    12541 cttcatcttc gccgcgctgc tcgtcttcct gttcctcacc tgggactgga cgattcagca
    12601 ggccggcctg caggtcatca tcgtcctcat ctcgctcatc gcccacgagg gggggcacgc
    12661 cgtcatggcg cgcctgatgg gctgccgcca ggtctccgtc tcgctcatca tgttcggcgg
    12721 ccagacctat cacgagccga ccaccaacgg gcgcaatctg ctgatcgttc tcgccggccc
    12781 cgccctcacg ctcgtgctga tgctgttcgg cttcggcctg ctgtttgtcc cggccgtgca
    12841 cgcctccgac gccggccgct ttgtctgcgg ctcgatcggc atcatgaacc tcatctggtt
    12901 catcttcaac atgctgccga tctttccgat ggacggcggc atgacgattc tttacactct
    12961 ggcgttcttc atgcgcgagc accgcgcgat gttcttcgtc tcgctcgtct cgatggccgc
    13021 ctgcatcctc gccgggctgg cgctctacct ttccggcctg caggtcacca tggcctatat
    13081 cttcctgatc ctcttttttc tgcagaacat ggccgtctac aaaacctacc gctgaggtct
    13141 attccccgcc gtcgcgcagc gcgatcagcc gccggcgcag cagcgcctcg cgcgcggcat
    13201 ccagcacccg cccggcgatt cggcgcgccg cgcctccccc gccgcgctgt cgggtgcggt
    13261 aaccgaacgt catgaacccc gggtgcccca gcgccatccg gtcgagccac gcctggtggt
    13321 cgttgcgcag gtcggcgcgc cggccgatcg cctctcggat cgcaggcgcc ggcgcctgct
    13381 cgacgcgcaa ccggtcgatc gaccgccggc ggaaccggtt ggtcaccggc cccgcccgct
    13441 gcatgtattc ccacaccacg tagcccaact ccacgggttg ctgcacgtag taatcggtcc
    13501 gccgcagccg gacggcgccg actcgcggct cggccggcgc cgccccccgg tacagcgccg
    13561 ccacgcgcgt gcaggagagg atcagatccc ccgccaggcg caggccccat gccgcgcgca
    13621 ggtgctccgc cagcacgccg atcagttcct gcgacaggcc gacggcgcgc tgcgtcagcg
    13681 tcagcagccg cgcctgccgt tccaccggcg ccaggtcgaa ataccgctcg cgcagcccgg
    13741 cgatcgcctc gctgaacgcc gccgcccgcg cgcgaaagcc ctccgcctgc cgcgcgccga
    13801 tccggtcgag cagcgcgaaa caatagctca accccttgac ccggctcatc agctcgcgtt
    13861 cgtcgatccg ctccagcgtc agataccgca cgaactccca cggatacgca tcgatcacga
    13921 tgtcgagcag gaacagcgtc aggtcgagcg gatcgtcgtt gctcagccgc gccggcaggt
    13981 cggcgtcccc ggtcccccag cggcgattca gttcgaaaaa cggcgcaaat ttgaaataca
    14041 gcggcaactc ccgcgcccgg acgaaatacg ggttgtgcag caacacattg tgatacctgg
    14101 gcgcgaccga ggcgtgaagc agcgcgcgca gcgcgtcgaa cgccgcgaaa tcatcacgca
    14161 cgacaaacaa cagatcgatg tcggacagcc ccggataccg gatcggcccg aaatcgtaaa
    14221 gcgcgacgac gccggggatc gaccggccgc gcgccgacag gtccgcgatc gccgcgtcgt
    14281 agtgcgccgg cgcgatcggc cccgccaccg gcgtccagcg gtagtcgcgc gcatcacggc
    14341 gcagcagcag ttcgcagtcg atcatcccag accccgtcgg cgcggttcgg ccggccaatg
    14401 attgacccgg ccccttcctg tctcagtccg ccggttcagc ccatcagttg ccgcgccagt
    14461 tcgcggcccg cttccgtcgc ggcgtcgccc cccatcagcc gggccagttc gtccagccgc
    14521 gccgacccgg tcagttcggt cacctcggtg aacgtctccc tgcgccgcgc gcttttcgat
    14581 accgccagat ggcgcgccgc ccgcgccgcg atcgccgcgt gatgcgtgat gcacagcacc
    14641 tggtgcgatt cgcccaattt ctccatgacg gcgccgacgc gaaccgccgt ctcgccggaa
    14701 atccccgcgt cgatctcatc aaacaccagc gtcgcgatcg catcgcgccg cgccagcgcc
    14761 gtcttgattg ccagcatcac ccgcgacagc tcgccccccg acgcgatctt gcgcagcggc
    14821 gccggcggca gccccgggtt cgccgccagc agcagctcca cgcggtccat cccgtcgccc
    14881 cccggcgctt ccagctcctc cagcagcacc tcgaaccgcg ccttgtccat gtgcagccgc
    14941 ttgagcagcg cccccacctc gcgcgcgaat cgctccgccg ccgcctttcg ccccgcgcgc
    15001 agcttctcgc ccgccgcgcg cagccggcgt tcggcctccg ccacccgcgc gccgatctca
    15061 cgccgctcgg cgtcgtcgcg ctccatccgc tcgcgctcgc ggcgcatccc ctccaccgcc
    15121 gcgaacaggc tctcctccgt tcccccgtgt ttgcgcgtca gccgccggat cgcttccgac
    15181 cgcgcgatca cctcgtccag ccgcgccgga tcggcctcga cctggtccgc gtaatcgcgc
    15241 agggcgtagg ccaggtcctc cagcgccgcc ttctgctcgg ccagcgtcgc gagccgttcg
    15301 gcaaacggcg gctcgacctc cgccagcgct ccgatgcgcc gctcgacttc ccgcagctgc
    15361 cccagcaccg acggctggtc gtcgctccct tcatacagca gcgcatatgc ttcggccgcg
    15421 ctgcgcgtca gttcgtccgc gcgcgccagc cggtgctcct catgctcgag ttccagcagc
    15481 tcgccggcgc gcggattcac cttgtcgaat tcgccgagct ggaactccag gtagtccagc
    15541 cgctgctcga attcccgcgc cgcctggtcc aattcatcgc gccggcggcg caattcgacc
    15601 acctcgcgcc acgcccgccg gtacgcttcg accacctcgg catgccccgc atgggcgtcc
    15661 agcgcatcgc gcgccgccga cgcgtgcagc agcgactggt gctcgtgctg accatgaaaa
    15721 tcaaccagca actctccgat ccggcgcaga tcggcgacat tgaccatctg cccgccgacg
    15781 aaacagcgat tgcgccccgc ggcgctcagc tcgcgccgca gcgtcagcgg ctcggccggc
    15841 gtccactcca gctccagctc gtccgtcagc agccgggtca ggccggccgg cggcgacggc
    15901 agtgcgaaac acgcctccac caccgccacg cgcgccccgg cgcggatcgc ctcggccgcc
    15961 gcccggtcgc ccagcgccag acccagtccg cccacaacga tcgacttgcc ggcccccgtc
    16021 tcgccggtca gtgcgttcag cccctcgccg aactcgatcg tcagcgcctc aatcgtcgcc
    16081 aggttttcga tgtgcagact ccggagcatc tcgaccccac gccaaattcc gtctctcgtc
    16141 cctcgtcctc ataatcgcct tcaacggccc gcccagaatc cggatctttt cctttactga
    16201 tcgcgatctt atttccgccg ccgatccaca tcgctgcatc gcggcccgcc ggcagcgtcg
    16261 atggtagcca gcggacgccc cgccccgcaa cgaggattcc tttccctcgc cgcgccggtc
    16321 atccgcgatc cccacgcccc gcgatttccg tttgcatctg cgccgcgtcc ccccttcaat
    16381 agggaccacg gcgcgaatac tcaccgggga gccggttatg ggcaaacgta cacgcgacta
    16441 tcttgcgctc gatctgggcg ccgaatccgg ccgcgcgatc gtcggccggt tcgacggccg
    16501 ccgcctgcgc ctgaccgagc tctaccgctt cacgcacgac tcgctgcgct tcaacggcac
    16561 gctccgctgg catgtcctcg gcatggccgc tcatctgcgc ctcgcgctgg ccgcggccgc
    16621 cgccgccagc cgcggcaacc tgcgcagtct cggcgtcgat tcgtggggcg ttgattacgt
    16681 cctcctcgat cgcaacgacg acctgctcgg ttacccctgg caataccgcg atgcgcgcac
    16741 cgaccctgcc ctccccgccg ttttccgcca gatccccccg ctcgagctgt tccgcctcac
    16801 cggcatccag cccctctggt tcaacaccct ctttcagctc tacgccgagg tgatgcagcg
    16861 gaccggcgcc atcgaggcgg cccgaaccct gctctttatc cccgatttcc tcacctggac
    16921 cctcggcggc gagaaagctt gcgaatacac catcgcttcc acttcgcaaa tgctcgatgc
    16981 gcgccgccgc aactgggccc ggggcctcct gcgccgactc aacctcccca ccgccctcct
    17041 gcccccgctc gtcgcccccg gcgccctctc cggccgcatc cgccccgagg tcgcccgcga
    17101 gaccggcgcg ccccggtcgc tccgcatcgt cgccgtcggc cgccacgata ccgcgtcggc
    17161 cgtcgccgcc gtccccgccg ccgacgccgg cgactgggcc ttcctcagct ccggaacctg
    17221 gtccctcgtc ggcacggagg aatcccgtcc ggccctgacc gagcaggccc tcgccggcaa
    17281 cctcaccaat gaggggggac tcggcggcgc ctatctcctg ctcgccaata tcatgggcct
    17341 gtggctcgtg caggagctgc gccgcgcctg gaccgaccgc cgcggccgcg cccccgacta
    17401 cgccgagctg accgccgccg ccgcggccgc gccctccggc gggccgcgcg tcgatccgga
    17461 ctggcccccc ttcggccgcc ccgatgcgat gcccgaaaaa atcgtgcgct actgccgcaa
    17521 gacccgccag acgccccccc ggaccccggg cgagtttgtc cgctgcgccc tcgacagcct
    17581 cgccgccgcc taccgccgcg cgctcgacaa cctggaggcc gtccgcggac gcccgatcca
    17641 ccggctgcat atcgtcggcg gcggttcccg caacgaactg ctcaatcagc tcgcggccga
    17701 cgccaccggc cgcgaggtcc tggccgggcc cgtcgaggcc accgccatcg gcaacatcct
    17761 cgcccaattg ctcgccgacc gcgagatcca ctcgatcgcc gaaggccgcg ccatcgttca
    17821 ggccagtttc cgccccaaac gctttctccc gcgcccgaat cgtcccacgt cctcgtcgcc
    17881 gtaatcgcca ccaacggtcc ggcccagaat tcggctctcg gctctcgtcc tcggctctcg
    17941 aaaaccgccg gttcatgaga agccattgcc cgggatcgcc cgcgccgcgc cggccgctac
    18001 tccagcatcc ccttcttctt catcttttcc accagcgtcg tccgcttgat ctgcagcagc
    18061 gccgccgcct tgttcttgat cccccccgcc ttctccagcg cccgctcgat cagttgcgtc
    18121 tcgaactggt cgaccagcgc gttgaagtcg atcccctcat ccgggatctc cagcccctcg
    18181 ctcggcatcg gcagcggccg cggcttgtgc ccggtgaacc gctcgggcag cgtctcctcg
    18241 gtgatctccc cgtccggatt cagaatcacc atccgctcga ccaggttctc cagctcgcgc
    18301 acgttgcccg gccacgggta ggccatcagc gcctcgatcg cgtcgcgccg gaaccccttg
    18361 acccgccgcc ccttcaactt gttgaatcgc tccacgaagt gatcgatcag gatgggtata
    18421 tcctcggcgc gttcgcgcag cggcggaatg tggatcggga tcacgttgag ccggtagaac
    18481 aggtcctcgc ggaaccgccc gtcggccacg tccgcctcca gatcccggtt ggtcgccgtg
    18541 atgatccgca cgttgacccg caccgtttcg gtcgctccca ccggctcgaa cgcctgctcc
    18601 tgcagcacgc gcagcagctt gacctgcagc ttcgggctca tgtcgccgat ctcgtccaga
    18661 aagatcgtgc ccccgtcggc cagctggaac cgccccacgc ggttcatcgt cgcgccggtg
    18721 aacgcaccct tcacatgccc gaacagctcg ctctcgagca ggtcctccgg gatcgccccg
    18781 caattgaccg gaatcagcgg ctgatcggcg cgcggcgaat tgtagtgcac cgcccgcgcc
    18841 accagttcct tccccgtccc gctctccccc agaatcagaa tcgtcgagtc gctgtccgcc
    18901 accgtgtgga tcagattgat catcgacttg attttcgccg aattgccgat gatgttttca
    18961 aatttgcccg accgcttcag ctcgcgccgc agcgtccggt tctccgtctg cagcgcctga
    19021 aactcccgcg ccttgtgcac cacgatcttg atttcgtcca ggtggaacgg cttcgtgatg
    19081 taatcgaacg cccccagccg cagcgcctcg accgccgact cgatcgaccc gtagcccgtc
    19141 gccacgatcc ccagcgtgtg cacgttcagc ttgctcagat ggcgcagcac ctccatcccg
    19201 tgcacgcgcg gcatcttcag gtccgtgatg accaggtcat acagcttgtc ctcgagcaac
    19261 gcgatcgcct cgtcgccatc ctgcgccgtt tcaacctcaa acccttcgct ttgcaggatg
    19321 ttggacagga cgtcgcggat gctccattcg tcgtccacga ccagaacacg ccccggtttg
    19381 tcgctggcag ccatgggatc ctccccgtga ggaacgaacg gcatgtccgg cagggagccg
    19441 gactgacaga ctcaagagtg ccaaaatttt gtcgaagctg caagctcaaa accaaaaaac
    19501 gactcggccc gtctaaaatt tggcgtcaaa gatccgaacc cccggcggcc gcggccgcct
    19561 ccgcgaccgc gacggaccct cttctgtcgc ccttcaataa cacgggggac cggcccccgt
    19621 ccccggtccg ccggtcccac gtagttgccc ctcgattcgc ctgcgtcggt cgtccctgct
    19681 ttatttccgc cggatcttcg tgaattcctc ggtctgctgc ttgatcgccc gttccaccgg
    19741 cagccgttcc atcgagctgg ccccgtagaa cccgtccacg tgctggcacc gttccagcac
    19801 atagcgcgca tcctccggct cggcgatcgg cccgccgtgg cacagcacga tcacgtcgtc
    19861 gcggatcgcg cgcgccgtcc gcacgatctc gtcgatcagc gtcacgcact cgtcgagcgt
    19921 cttcgccgtc cgcgccccga tcgaccccga cgtcgtcagc cccatgtgcg ccaccagaat
    19981 gtccgccccc gcccgcgtca ttttctcggc gtcggtcgtg tcgaacacat acggcgtcgt
    20041 cagcaggtcc agttcgtgcg cctgccggat catctcgact tccttgtcgt agcccatgtt
    20101 ggtctcttcc aggttcgcgc ggaacacccc gtcgatcagc ccgaccgtcg ggaaattctg
    20161 cacgcccgcg aagccgatcc gcttcacttc ggacagaaat tgcggcatct gccgcatcgg
    20221 gtcggttccg cagacccccg ccagcaccgg cgtctgcttg accaccggca gcacctcgcg
    20281 cgccatctcc atcacgatcg cgttggcgtc gccatacgac agcagccccg ccagcgaccc
    20341 atgacccgcc atccggaacc ggcccgagtt gtaaatcacg atcagatcga tccccccggc
    20401 ctcctcccat ttggcgctga tccccgtgcc ggcgccgccg ccgatgatcg gccggccctc
    20461 ggcgatcttc ttgcgaaatc gattcaggat ttccttgcgc gtctcagcca tcttgcttcc
    20521 tccattcaat gatcttcgtc cctcactcaa ggaccgtcgc tcgatcctcg tcctcgtccg
    20581 cgtgatcgcg ccccacggcc cccccccccg gaattccgct ctcgggtgtc tttcgtgatc
    20641 cgtgttcgtc ttcccccctc aagccatctc ccgcttcacc tggtcccaca cccgccgcag
    20701 caactcctgc accttgaccg gcatccccgt ccggctgctc tcgatcgccg cgaagatcaa
    20761 cgccaccgac tgcaggtttg cctcgacgtt cgtctccatc gccggtccgc cgtcgagcca
    20821 cttgacgaat tgctccacca gccacgcgtt ctgccatttg ggctgctcca gcagcgggat
    20881 cgtctcgccg tgcccctcgc gcgcgctcac gccgctcttc cccgccggca gcgcgaactt
    20941 ctccagccgc cgcgtgtcca ggatcagcgt cgccttctcg cactccgccc ggatgtattc
    21001 attcccccag caattcagcc ccgccgtgtt ggttttcgcc ccctcgtaga agatccgctt
    21061 gccgttggcc agccgcatcg tcaccagtcc ctgcgaatcg cccgcatatt ccccccaccg
    21121 cgggttccac gtctgcgcgt agatctcctc gcacagcgcc ccgcccatgt cggccagcag
    21181 atccagatga tgcaccgccc cctccaccat caacggatcg gggatctcgt ggcggaactt
    21241 gccccagctc gcgaacgccc ggcacgggca cgtgaaccgg ctgacgatgt agtccagctc
    21301 cccatgccgc cccgaccgca attcctcccg cagcgtcgtc ttgtcctggt cgaaccggtg
    21361 actcatcgtc accgccatct tcttccccgc ccgcttgacc ttctccacga tccggcacga
    21421 cgcctccagc gtgtcggcga tcggcttctc cgacaggatg tgcaggtcat gcgccagcgc
    21481 gtggtccacc atcgtctcgt gatgcgccgg cggcaccacg atcgtgcaga agtcggccgg
    21541 gttctcatcc agcgcccgcc ggacgtccgt atagcaccgc tcggccggca ggttcagatg
    21601 cttgcgcgca tgggccaggt tgtccggatt gatgtcgacc gcggcgacga cctcgatcag
    21661 cccctcccgc acattggccg gcaggaacca ctcgcaccag cacgccccga atccgcccgt
    21721 cccgatttgt atcacgcgat aagccatgtt cacgtctccc gttcagcgcg ccgccgcgtc
    21781 cgctttcaga ttcttcagca gttcctgggc gcacgcctcc gcgaacgccg catcgttgat
    21841 gttgcagtcc agctcgatca ccggcacgcc cggcttcaaa ttcgccttca gctcgtcgaa
    21901 cagcgccttg tccgcttccg gcgaataaaa cgccttgccc tccgcgctga tgacgctgat
    21961 cccccgcagc ggaatcagca ccgacaccgg cgccgtgctc aggttgatct tctccgccag
    22021 gatccggccc aactgccggt tctcctccac gttcgtccgc atcagcgtca cgttggggtt
    22081 gtgctgatag aacgtccgcc ccttgaactt ctccggcacc gtctccggcg cgccaaaatt
    22141 gaccatgtcc aggcaccccg gcacgacgat cgccggcacc ccgcggcgcg cggccgcctc
    22201 cagccgtgtc ggccccgccg cgaacacgcc ccccaccagc tcgtccgccc attccgtcgt
    22261 cgtgatgtcc agcgacccgg cgatcagccc cgcctcgatc aggctctcca tcgtccggcc
    22321 gcccgtcccc gtgcagtgga acaccagcac ctcgtagccg ttctcttcca ggatctggcg
    22381 cgcccgctcc accgccgccg tcgtgttgcc gaacatcgac gccgtcacca gcggcttgtc
    22441 ctgacccgcc gggatctcga tctccaccat cccgcagacc gccccggccg cccgcgcgaa
    22501 cacctcgcgg ctgatccggt tgatccccgc cacgtcgacg atgctcggga tcatcacgat
    22561 gtccttgacc ccgacgtaac ccgaaacgtc cgtccccgcc accgtcgaga ccatcacctt
    22621 gggcacgccc agcggcagcg cgcgcatcgc attgcacgcc accgacgtcc cgccggttcc
    22681 gcccatcgcc agaatcgcgt cgaacttccc ctcggcatgc gcccgggacg ccgccgccgc
    22741 cgccccccgg ctcatcagcg cgaccgcctc gccgcgatcc cccttgcccg ccagcgccgc
    22801 cacgtcgccc ccgccggcgc gcgccacttc ctcgcgcgtc aggtcggccg cgatcgccgg
    22861 ctggcccatc acccccacgt cgatgatgaa cgtcttgtgt ccgcgcgcgt ggatcaggtc
    22921 gcgcacatac ccatactccg cgcccttcgt atcgagcgcc cccagcaacg cgatcgtctt
    22981 gcccatcggt tcacctcact cgctcggatt gttccgcccc aaaactcgcc cctcgtccct
    23041 ttcgtgtcgc ttcgcgtcgt tcgtgatcca gatcggtagc ggaccgaacc gcttctcagc
    23101 gcgccggcgc cccgcgccgc tgcgccagca gccacgcata cagctctgga ttcgcatacg
    23161 tctccgtcca cgaatcatga tccgcgtcgg gatacaccgt gaacttcacg ttcccgccgt
    23221 agcgccgcag cgcctccacc atccgctccg attcgctcag cggcaccacg ctgtcgcgcg
    23281 ccccgtggaa cacccacacc ggcagatgcg ggatccgccg ggtgtagaac gccagcccgc
    23341 cgccgcagac cggcgcgatc gccgcgaacc gctccggctg cgcgagcgcc agcccccacg
    23401 tcccgaaacc ccccatgctc aaccccgtca cgtagatccg gtcccgatcc acccggtagg
    23461 ccgactccac gtaatccacg aacggaatca gcgtctcggg ctcccaccac gcatccgccg
    23521 gacactgcgg cgcgaccagg atgaacggga aacgctcccc ctcctggatc cgccgcggcg
    23581 gaccgtgcag cgcgaccttg ttcaggtcgt cgccccgctc gcccgcgcca tgcaggaaca
    23641 gcaccagcgg ccagcgccgc tccgcctcgg cctcataacc atcgggcaga tacgccagat
    23701 accggaacgc cagctccttg cgcagcgtca cccgggccga cgcgtgatgc aggcccggcg
    23761 ccgtgttcag ccgttcgttc atcaagctgc aaccgcctgt cagggatacg ccgatcacga
    23821 ggccgatcac cccggccacc gccacgccgc ttcgccgcac gccgacccgc ttgcccatca
    23881 cgccgcccgc cacccttctt cgaacacgcc ctccatcccg cccatccgtt cccaccgaca
    23941 agtctcaaca tcactaggtc ctttcctccc aaacgtcaag ccggatcacg cgataattcc
    24001 cgaaaacccg tccggtcggt tccgcgtagg gatgttcgcc ctcgatcggc cgctggcgcc
    24061 aggcctcgat gcgctcgtag agaaaggagg gacgcgcgga gcaatgtgcg gttgttcggg
    24121 agccggccag ctacccgaat caagac
//
LOCUS       DAOPCO010000033        24018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2259_length_24018_cov_5.364729, whole genome shotgun sequence.
ACCESSION   DAOPCO010000033 DAOPCO010000000
VERSION     DAOPCO010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 24018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24018
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2259_length_24018_cov_5.364729"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..1146)
                     /locus_tag="PLS90_04320"
     CDS             complement(34..1146)
                     /locus_tag="PLS90_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPK01662.1"
                     /translation="MNDTQHRWTVLLLAVGLTWGLASPALAVRIKDVTHLQGMRDNQL
                     IGYGLVVGLDGSGDGSSTEFHVRSLVAFLRRNGVTVDPDLVKVDNVAAVIVTADLPAY
                     ARPGQRIDVTVNSIGDAESLQGGTLLMTPLKGPDGNVYAVAQGAVLVGGFSAGSGGAG
                     ASKNHVTAGRVPGGALVEASPPTVLAGHEELNLILDNPDFTTAQRVAEVINGEFSEGT
                     AQAIDAAGVSVRVPSQYANDLAAFMAELERLPVHVDQRARIVIDERTGTVVMGEEVRI
                     STLAIAHGNLSIQIARTNEVSQPYPDSFGETTRFSNVDLAVQEEGDQLTLVPEGVSLG
                     EVVQSLNAIGVTPRDLISILQAIKAAGALQAELVVQ"
     gene            complement(1225..1992)
                     /locus_tag="PLS90_04325"
     CDS             complement(1225..1992)
                     /locus_tag="PLS90_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPK01663.1"
                     /translation="MNRLVSRLLTLSLACAPALLGGCVVVKEAYKPNPQPAAYTPPVP
                     PELDRAWQPQPMSEGSLYAAATPNLFGDDRASRPGDIIQVRVMQRNKGTKSANTETAR
                     KSGISARIKYFLGLEDNINNVTGYLDNNTPEPKPGTEGWDPVDLLSAESTTTFSGEGG
                     TERNDNLEATVSAIVTDVMQNGNLVIYGHQTVTLNNEASVLTVQGIVRPSDLDVDNSI
                     LSSRIANARIEFTGSGVITDKQHPGWANRIFSWVWPF"
     gene            complement(1992..3074)
                     /gene="flgA"
                     /locus_tag="PLS90_04330"
     CDS             complement(1992..3074)
                     /gene="flgA"
                     /locus_tag="PLS90_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HPK01664.1"
                     /translation="MKHPRPERLATEGPRPASPARPRLSRHDAQFVVILIAMLIAILL
                     MHFVSYGATGEELPLPPPPVLDDPDLLVVSADAEASLIPVDGPPAAEPEPAAVPSIDL
                     AALHEMDTAFADYTAAQPEAPPAEGTWGEPLTTQEVRAMLADVDRTAPSRLTLESMLM
                     EQLRASLPGGGSDYIIERATLPDALDLPHEGWSVKYSFRLPANGLGATPFSGVVSDGA
                     GTVLRRFSGSVWLDREARGIQPTRMIRRGEVLGKADVQAVDARLSALPRGALTRLELV
                     ENTVARQELRPGQWLTDAMLDVPDAVKRNHPVTIRLERGPLQITTHGVAREAGALGEV
                     IRVENLQSRRVIFARVLSSDEVQVIY"
     gene            complement(3099..3884)
                     /gene="flgG"
                     /locus_tag="PLS90_04335"
     CDS             complement(3099..3884)
                     /gene="flgG"
                     /locus_tag="PLS90_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HPK01665.1"
                     /translation="MRALYVAATGMHTQQLNIDNIANNLANVSTTGFKRGRVDFQDLL
                     YEAVQSPGTNASINTEIPTGINVGHGSRVSSIQKLFTQGSFQNTGQPLDLVIEGNGFF
                     QITLPNGEPAYTRDGSLKLNSQGQLVTSNGDLLSPAITIPADAENITIANDGTVSVIQ
                     PGQAAPAVVGNITLTSFSNPAGLEAIGQNLYRETQSSGTPTTGTPGADGYGSLRQGFL
                     EMSNVSIVEELVSMIVAQRAYEINSKAITTSDEMMQTATQLKR"
     gene            complement(3917..4678)
                     /gene="flgF"
                     /locus_tag="PLS90_04340"
     CDS             complement(3917..4678)
                     /gene="flgF"
                     /locus_tag="PLS90_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HPK01666.1"
                     /translation="MKEIHLTIAAATGQERALDRIANNLANVSTPGFKKDRAVFLNYL
                     AGGALQAGLTAPSGGADPAKSAAAWPTAGTAFTDLSAGPIQHTQQPLDLAIEGDGYFM
                     VEVEGQQAPRYTRAGNFRLNAAGELTTAAGRRILGDSGEPITIDPGLGAPEITETGEI
                     FVGGQQVGRLGMVSFSDPSVLRKWGEGLLMAPPGVEPGESTGRIRQGYLEGSNVNPIE
                     EMVRMIQAQRIYETQQKALQTADELTEQRIRAARE"
     gene            4920..5867
                     /locus_tag="PLS90_04345"
     CDS             4920..5867
                     /locus_tag="PLS90_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPK01667.1"
                     /translation="MTPSPIPLGIGIDGGATAFKWTVGAPGGDLRRGTAPGGNPHVMG
                     WEPWLAALGDAIDGALRAAGGQRGELGALGLGMAGVDRPGEPERIQAWLRRHFPNLNR
                     IWIGNDALTALRAGAGKLEGLVLLAGTGSFCLGIARGGRRARAGGWGHVLGDEGSGWW
                     IGREALAALCRMADGREAPTALLPALLSRLDLKDASELIPWLAARDAAAQKRGIAALA
                     PAVLAAARTGDAAAERIVVDAAGRLAALVRAVANALAGEFDPPVPVICSGGLFKDEAF
                     LARVAGHCGPGFACRAATAPAADGALALAWESRAPPAAD"
     gene            complement(5904..6056)
                     /locus_tag="PLS90_04350"
     CDS             complement(5904..6056)
                     /locus_tag="PLS90_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01668.1"
                     /translation="MVCRHCRVKMKEARRVFHKQRKWICPRCGRARMQTARPRGQARG
                     KADGER"
     gene            6237..7073
                     /locus_tag="PLS90_04355"
     CDS             6237..7073
                     /locus_tag="PLS90_04355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPK01669.1"
                     /translation="MAMIRTAEPRARVRALIQLQFLLAALTGLSGCTTFRVASESMFM
                     ISPEEERQIGQQVKSEIDGQVQFVNDSLVVNYVRDVGRTLVAHAPESAFDPQFFVIAN
                     PEINAFAIPAGSIYVHTGLISAADDEAEMAAVIAHEMGHVIRRHSARGLARNTGAQLA
                     GQLLLGENYGSMAQLLSSVGLLNYTRQDEAEADRIAVATLHRAGYDPDALATFFAKLK
                     ERYGDRGQIATFFASHPPTTERINSVRAMVDQLPEAEYRRPTSDLRRVQKRLEELGFA
                     AE"
     gene            7318..9009
                     /locus_tag="PLS90_04360"
     CDS             7318..9009
                     /locus_tag="PLS90_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPK01670.1"
                     /translation="MVPSLTSLKPAHLKRYAEIARLLLRYGRSDLVRQSGLYQLVAEE
                     QQRDLAAAPAEARELANDLERLGPTFIKLGQVLSSRADLLPFEYMEALSRLQDNVKPF
                     PYEEVERIVEDELNVSIHKAFQEFNVRPAAAASLGQVHHAVLRSGQPVAVKIQRPGVR
                     EQVTEDLDALRRIAETLDAHTELGRKYEFTLLLDDLRRLLIRELNYREEASHLRILAD
                     GLCDFPSIVVPRPIDDYTTSRVLTMQYVRGYKVTSISPLVRLEIDGHHLLEELFRAYL
                     HQIIVIGLFHADPHPGNIFLTSDHRLALLDLGMVGRVPANRQDQVLRLLLSISQGLGE
                     ETAEILLEMSEKRENNNVEGFMQGIEDLISDVAGGALGDMNIGRSMVQLQRVAADNGV
                     RLPREVRMIGKALLNLERVGAVLCADFDLRAEIRDHTAGVMRHKLLEAVAPGNLLKSF
                     ADIKRFVLKLPAELNQILSVVTRNGLRVRVDAIDEDRLMAGFQKVANRITVGLVVAAM
                     IIGAALLMPIQTGFVLFGYPGLAILLFLFAALIGIYMVISILWKDIKEPRRPRRR"
     gene            complement(9053..10402)
                     /locus_tag="PLS90_04365"
     CDS             complement(9053..10402)
                     /locus_tag="PLS90_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK01671.1"
                     /translation="MADNHKSAYAVLGLHKGASLDDIKKAYVQLVKKYDPERHTDRFM
                     IVQNAFDRLKTPASRAREDVRAFNYIRGEFFFSAEEKTQLADAQIDQGLRQLEEKINS
                     GQVDRAAATPKLIQGLMMRSYKRVQKKLWAEAIEDWQRVLELDPAHRRAKNNLLYSYI
                     TLGYSYASHGLYDEAVDVLKRAATMNPDGTEIIHNLALACEHAGRVEEARRYWQEVIK
                     RWRAQLNQDPDNEYLKACLVEALKEKGQSGEAPAEEKAEGGAPAQARGTQEPSAPRAA
                     HSVEDLREILRLNPNDFESHFRLASLLYNEQKWEEATKELEEMQRRFPRNIEVLNLLG
                     WAMLNSGRIKEAFMVWRRARTMDPKNPQITESLIKANMSIGRTLRDKGLYTQCLVHFK
                     ELTRYLPENDEVHYEIGLTYQLKGDERSAFQEYQKVLKINPRHRGARHGLSSLKLRR"
     gene            complement(10699..11916)
                     /locus_tag="PLS90_04370"
     CDS             complement(10699..11916)
                     /locus_tag="PLS90_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK01672.1"
                     /translation="MNTREQVINHVRHLVDLTFLDDEMRARPTKVQTAVSTTCNLKCP
                     LCLRESMGIRENLFMDFGEFERKLPELEEAACVSLFGLGEPFLHPRFFEFVEACKARR
                     ISVATSTHGMSLTPEVRERLVAVGLDDLHISMDGTTRAVFERLRAGAKFDTVVEQVTA
                     LAALKRERGVDRPRLNVNMTLSKYNVRQAPAMARLAHRMGCQSVSYSSAVAYKPEDED
                     ISVLDTPIIERMLQRAKVLADRLGLGFSFWRQKAVGYRGELYDQTSAYGCVQLNGDMT
                     IERNGRMKMCCYIEEDIEDVWGEGPAAAFNGPNMRAARRKLREGRVRTECQGCLYLRE
                     RTPFWVQSLLNEAQRVVTHNHLLTEEDRAELRRLLAETQARKDAMWPEHGGGASAQDS
                     AGAALAHEVATVY"
     gene            complement(12034..14880)
                     /locus_tag="PLS90_04375"
     CDS             complement(12034..14880)
                     /locus_tag="PLS90_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HPK01673.1"
                     /translation="MQALRRRIWFGGMLFAAAIVGACARYGMPSEAEMDALQASIARE
                     MVATAEGRPRLYPTKFATSEPIIAGFNVRDYGAIGDGRIDDTRAFQEAFDAAGRVGGA
                     VVFAPAARYLIEGRLTLPRGVTLRGEYDPARVNEGTLLLATGGRGSEAGPAFITMELC
                     TGLVGLSIYYPEQEAGAPAAYPWTIEQTGVDSITVRDVTLVNPWQGVKIGPQPNELHF
                     LRNVRMTALKTGVLIDATTDIGRLERVVISPGVWEKSGLLAGGLFSRNGALRRHLKTH
                     ADAIVMQRSDWQYFTDVAIDGYRNGLTLRDGPNGASNAQAFNVWISDCHTALNAVQTN
                     YIGFAMTGSHLEAESGPGAAALRTAPDFTGILQLNGTRIACKAGEAAVVLEGDGVVSL
                     QNCSLTAADCPSIEARAGHLTVEACAFSTTAPIHLAETMRSAILAGNGIPADLSGRAY
                     ERSARRWKDRFLANSEDSRLPKIVSGNPGFVIHGAFDWEEDRFVGDRVVKVPWPEFPR
                     PGSSDVFVATAAPFSAPADGESDAAAAIQAALDAAGEKGGTVYLPAGRYRLEGPLTIP
                     PGVELRGIWEVPHHTMGEGTVLMAVAGRGEAEGTPLLNLRPGAGLRGLTVYYPEQDFR
                     EPVAYPWAVRGQGHGVYALNVTLSNAWRGIDFGTHDCTGHVIDYAAGAPLRTGIFIGE
                     SKGGWVSNTQFNPHYYARSPYPGHPDEGRREWQVWWNQQKAQLDALLLAGADNQWLVN
                     NFVYGSRHGLRFTEPQPAIPVAAGSSISGEVSVPERPRAQTVVIGHGSDGTAQGVRIE
                     AVGARGLEFINFQAAVFAAREEAYIAVKPAAPDAVAGPIRFFNSLFWAAPKFGIDVAG
                     GEVQVRQAHFVVPGETMARVAGDAGGTDPGGRLTLKSIYVQAPTERHVLLEEGAAGMR
                     LTTGLVAGEFRLENRSGVEPEMGVVVERETTEGL"
     gene            complement(15088..16863)
                     /locus_tag="PLS90_04380"
     CDS             complement(15088..16863)
                     /locus_tag="PLS90_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01674.1"
                     /translation="MESRGRLAACLGRVVMVQHPPDPHPDETAAGGRLAGGASGRRLR
                     VALFVIGLLVYGVGRLHDLSFDIDELHYLEGARNLARGAGFTKNNGESLELFSPLYPL
                     CVTPIYMVTGRIFGSAFAASAAIAAGAIAVWIAFVYAWSGGAGGDRWFPALALFILFT
                     NRFWTGALLRLGPEGLLLLALGAELLALTRLADGATGRRMRLVWAVVAGLGAAAAYVA
                     RPDGLVYSGLAAVWFLVRLRGLRRPFPAAAAAAGLIVAIVAASPYLLYLRAATGEWTL
                     TAKTEKNLLHGVTYTEDRLAYEQIIYGGWEEEQEEPEARRRAERMAEEGLWGMLVHYP
                     RELAGRVWVNTGEIVEALNNFIGLAWGWPLLALGALALACGPAAVRRGELAALGVFLP
                     GLAILLPFHVEDRYVLVPAVLIAPPLLAAGVLWLVERGRRLREGRWKRRAVYVLALAL
                     YVGMAQETAPEDMFDKPDDEDRHYDQLAQWAREAGLAPGTVVSRKNKFNFRLNWPREA
                     LPYVTNHGDLLAFLDEVNARYLLIEEDQVLTKRPELGYLVRPTAETTAGLKLLRSLNN
                     PGHEDSRLVLYSVDSWEPGEENGEE"
     gene            17175..17987
                     /locus_tag="PLS90_04385"
     CDS             17175..17987
                     /locus_tag="PLS90_04385"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPK01675.1"
                     /translation="MRSFLPLVVLAAFSLPLPACAQEAPAPPSPTDPAGPVEILLHAD
                     PPTTGAVARPQLPPDEFTDQRAWMVEHQIRARGVTDETILAAMRRVPRHQFVRPDDLA
                     RAYGDHPIPIGHNQTISQPFIVATMTEMLQLTPESRVLEVGSGSGYQAAIAAELAREV
                     VTIEIIGDLARLAAHRLRRLGYTNITVLHGDGYFGWPAGAPYDAIIVTAAADHVPPPL
                     LAQLKPGGRMAIPVGRAGWAQNLLLVEKDANGAVSTRNLLPVQFVPLTRERR"
     gene            17989..20379
                     /locus_tag="PLS90_04390"
     CDS             17989..20379
                     /locus_tag="PLS90_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01676.1"
                     /translation="MSPDRPNPSAADYLSVAAVSAALLAFEILLLRLFSLTQWHHFAS
                     LAVALALLGFGAAGTLLTLLADRARRWGDPLHAGATAAAGLLLLAILPLNRLIEIHPL
                     FIIWDVRQLLRLLALDLAAFLPFMAGAVAIGQVFVRWPAATRRLYGANLLGSGAGSLG
                     AVALLATVGLPAALMLLGGFLLLTAALFGCLPRPGARPRARACGGLAAAGLLLLVAWA
                     ALPGSPAIRVPQFTELNFRLSLPDAEVIAQHHGPRGLSTLLRSSSNRLAPGLSLQWFS
                     VVPSHDVLILDADRAIPLPAPAAFGDLDYLDAALGALPWRLRPRATTLVLGTSAWLMP
                     LHARRADAADLVWVEDDPRISSAVARTDPGLAARLRRQAPRSALATLRKQFSLIVVET
                     AAGGGEALAESPLLTVDALAAMSRRLAPDGFLVIPLPLANPPRHLPRLLAMIHQCLVQ
                     GGADDPPRHVIALRALHDALVLVAPAPFSPGDIARARAFADQWGFDLIWHDGLDPADA
                     NRHARLAEPLYYAAAAALLAGRDTPTPAARFYNRLPATDARPYFWYSVDWTRLPDLLR
                     TLGRRGLTFVDWDALMQATALAAAALLALLLIVAPLGRLPAARRPLGHASIWLYFSAL
                     GLGYLMLELAVFQRATLLLGSPVVAAAVIFATFLIASGMGSLTAPEAAIPSIAARLFA
                     FLALGVVLAALVLWGAGDRLLALRALPRAVVLVFAAAPMAWAMGRPMPWGLRRLGAQP
                     DWIPWAWGINGFTSVLAAPLAALVSVRWGQPVTWTAALLGYAVAAAVAFHWSRPHP"
     gene            complement(20660..21694)
                     /locus_tag="PLS90_04395"
     CDS             complement(20660..21694)
                     /locus_tag="PLS90_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01677.1"
                     /translation="MRISVIIPCYNAAGTLGECLAAVARERRSGAPIAEVIVVDDAST
                     DGSRQIAAGAEGVRLLALERNGGDCAARNAGARAATGEILWFLDADVTAAPGSARIVA
                     EFLTDRADVDAVIGSYDDRPAAAGFCSQYRNLLHHYVHQRQGPSVSTFWTGCGAVRRA
                     AFERLGGFDDSFWPGAPIADMEFGYRLRRGGGRIALRHDLLVRHHKRWTLGSIVRTDL
                     FGRAIPWTVLLQRYPELGRGELNLGWREKGAVASVAAGLAAGAAACAAAVFARGVGLP
                     LMLPASGCALFAVMLHAPILAFLQEKKGIRFALGSLLLLLIYDATCAVGYLAGRVCHA
                     LGYKPRRKSA"
     gene            21935..22741
                     /locus_tag="PLS90_04400"
     CDS             21935..22741
                     /locus_tag="PLS90_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01678.1"
                     /translation="MTAIKPDLASHWSGPPRADWRLPASWHQYSAELHGRLLQRWRGI
                     LPPGTPILKTDLFEEAQADATPAARIADLGWRPLGMDLGAGIARAARARAPQLAAVVV
                     ADARRLPFRTASLPAIFSNSTLDHFHNLADLDAALAELHRVLAPGGRLLLTLDNPCNP
                     LVGLRNALPAALTDRLRMTRYYVGRTLGPRAARARLLALGFQIEAAGTLMHAVRYLAL
                     RLLRRRERAGADAAPLLRRMLAMERLARWPSAPLTGHFIFLEAVKPCSNA"
     gene            22726..>24018
                     /locus_tag="PLS90_04405"
     CDS             22726..>24018
                     /locus_tag="PLS90_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01679.1"
                     /translation="MLERLSNLRHAGRMLAALPREARFPFRPRAEIEARRDARLRETI
                     RLACRAVPYYRERMAALGLTPEEFRSIDDLARLPVTPIDDLRDAPREFLSARVNPDRC
                     QLLRSGGTTGNEKWVWHDPASLLMNVAFSERQEFVIARFVGHRLRQRQLVINSTSSVS
                     STVRAAYRRRLALLRPLGPRRVRIGIMEPFMDQVAALNRLRPEVLITNGLFIGDFYER
                     LQRAGLAPHRPRVIVAGAETLPADQRRRIEHDFGVPVIMTYQSTESLKIGFECEYRRG
                     YHLHEDLCVVRLLDADGRPVPPGAVGRIHITNLVNRATVLLNFDQGDTGRFLDEPCPC
                     GRTLRRLELVGSRQWPLLRALDGRTLHGADVIRPVKEHTAVTRVQILVERPDLWRVLY
                     TDPRNETTAAWRRDTARRLERAVAGPGITVRLEQRDQLE"
BASE COUNT         3542 a         9396 c         7031 g         4049 t
ORIGIN      
        1 gatgccgatc ggtttgattt gcatctcggc catctattgc accaccagtt cggcctgcag
       61 cgcgcccgcc gccttgatcg cctgcaggat cgagatcagg tcgcgcggcg tcacgccgat
      121 cgcgttgagc gactgcacca cttcgcccag actcaccccc tcgggcacca gcgtaagctg
      181 gtcgccttct tcctgaacgg ccaggtccac gttgctgaac cgcgtcgtct cgccaaaact
      241 gtcgggatac ggctggctga cctcattggt ccgcgcgatc tggatcgaca ggttgccgtg
      301 cgcgatcgcc agtgttgaga tccgcacttc ctcgcccatg acgaccgtcc cggtccgctc
      361 atcgatgacg atccgcgccc gctggtccac atgcaccggc agccgctcca gctcggccat
      421 gaacgcggcc aggtcgttcg catactggct cggcacgcgc acgctgacgc cggcggcgtc
      481 gatcgcctgg gccgtgccct ccgaaaactc cccgttgatg acctcggcga cgcgctgggc
      541 ggtggtgaaa tccgggttgt ccagaatgag attcaattct tcatgaccgg ccagcacggt
      601 cggcggcgag gcctccacca gcgcgccgcc gggaacgcgg cccgccgtaa cgtgattctt
      661 gctcgcgccc gcgccgccgc tccccgcgct gaaccccccc accagcacgg ctccctgcgc
      721 gacggcatac acattcccgt cggggccctt cagcggcgtc atcagcagcg tgccgccttg
      781 caggctctcc gcatccccga ttgagttgac cgtgacgtcg atccgctggc cgggccgtgc
      841 ataggccggc aggtcggccg tcacgatcac cgccgccacg ttgtccacct tcaccagatc
      901 gggatcgacg gtgaccccgt tgcggcgcag aaacgccacc agcgagcgga cgtgaaactc
      961 ggtcgaggaa ccgtcccccg agccgtcgag cccgacgact aggccgtagc cgatgagctg
     1021 gttgtcgcgc atcccctgca ggtgcgtcac atccttgatc cgcaccgcca gcgccggcga
     1081 agcaagcccc caggtcaggc ccacagccag aagcaagacg gtccaccggt gttgcgtgtc
     1141 attcatcgta ttcgtcctcg tccctcgtcc tcggctctcg aaatccccta cgccccggcc
     1201 atctccaggc aatcccctct ccccctagaa cggccagacc cacgagaata tccggttggc
     1261 ccaccccggg tgctgcttgt cggtaatgac gccgctgccg gtaaactcaa tccgcgcgtt
     1321 ggcgatccgc gacgacagaa tcgaattgtc cacgtccaga tccgacggcc gaacaatccc
     1381 ctgcaccgtc agcacgctcg cttcgttgtt caacgtcacg gtctgatggc cgtagatcac
     1441 caggttcccg ttctgcatca cgtcggtcac gatcgccgag accgtggctt ccaggttgtc
     1501 gttgcgttcg gtcccccctt cgccgctgaa cgtcgtcgtg ctctcggcgc tgagcagatc
     1561 caccggatcc cagccctcgg ttcccggttt cggctccggc gtgttgttgt ccagatagcc
     1621 ggtgacattg ttgatgttgt cctccagccc gaggaagtac ttgatccggg cggagatccc
     1681 cgacttgcgc gccgtctcgg tgttggccga tttcgtcccc ttgttgcgct gcatcacgcg
     1741 cacctgaatg atgtcgccgg gacgcgaagc gcggtcgtcg ccgaacaggt tcggcgtggc
     1801 ggccgcgtag agcgacccct cgctcatcgg ctggggctgc caggcccggt ccagttccgg
     1861 cggaaccggc ggcgtatagg ccgccggctg cggattcggc ttgtaggctt ccttgaccac
     1921 cacgcacccc cccagcaacg cgggggcgca ggcgaggctc agcgtcagca atcgcgagac
     1981 gagacggttc atcagtagat cacctggact tcatcggacg ataacacgcg ggcaaaaatg
     2041 acgcgccgcg attgaagatt ctccacgcgg atcacttcgc cgagggcgcc ggcctcgcgc
     2101 gccacgccgt gcgtcgtgat ctgcaacggg ccccgctcca gccggatcgt gaccggatgg
     2161 ttgcgcttga ccgcgtcggg cacgtccagc atcgcatcgg tcagccactg gccgggccgc
     2221 agttcctggc gcgcgaccgt gttttccacc agttccagcc gcgtcagcgc gccgcgcggc
     2281 agcgcgctca gccgcgcatc gaccgcctga acatccgctt tcccgagcac ctcgccccgc
     2341 cggatcatcc gcgtcggctg aatcccccgc gcctcgcgat cgagccagac cgaccccgag
     2401 aaccggcgca gcaccgtccc cgcgccgtcg ctcacgacgc cgctgaacgg ggtcgcgcct
     2461 aggccgttgg ccggcagccg gaaggaatac ttgacgctcc agccctcgtg cggcagatcg
     2521 agcgcgtcgg gcagcgtcgc ccgttcaatg atgtaatcgc tcccaccgcc cggcaggctg
     2581 gcgcgcagtt gctccatcaa catcgattcc agcgtcagcc ggctgggcgc cgtccggtcg
     2641 acatcggcca gcatcgcgcg gacttcctgc gtcgtgagcg gctcgcccca cgtgccctcc
     2701 gccggcggcg cctccggctg agcggccgtg taatcggcaa acgccgtatc catctcgtgc
     2761 agcgccgcca ggtcgatgct cgggacggcc gccggttccg gttcggccgc cggcgggcca
     2821 tcgaccggga tcaggctggc ctccgcatcc gcgctgacga cgagcagatc ggggtcgtcg
     2881 agcaccggcg gcgggggaag cggcagctcc tcgcccgttg ccccgtacga gacaaagtgc
     2941 atcagcagaa tcgcaatcag catcgcgatc aggatgacga cgaactgcgc gtcgtgccgg
     3001 ctcagccgcg ggcgcgcggg cgacgccggg cgcgggccct cggtcgcaag tcgttcaggt
     3061 cgggggtgtt tcatcgcggt ggctcctggc gtctgccctt agcgcttgag ctgcgtggcg
     3121 gtctgcatca tctcgtccga cgtcgtgatc gccttggagt taatctcgta cgcgcgctgc
     3181 gcgacgatca tgctcaccaa ctcctccacg atgctcacgt tgctcatctc aaggaacccc
     3241 tggcgcagcg agccgtagcc gtcggcgccg ggcgtgcccg tcgtgggcgt cccgctggac
     3301 tgcgtttccc gatacaggtt ctggccgatc gcttccagcc ccgccgggtt ggagaagctg
     3361 gtcagcgtta tattgcccac caccgccggt gccgcctggc ccggctggat caccgagacc
     3421 gtgccgtcgt tggcgatcgt gatgttctcg gcgtcggccg ggatcgtgat cgccggcgac
     3481 agcaggtcgc cgttgctcgt caccagttgc ccctggctgt tgagcttgag cgagccgtcg
     3541 cgcgtgtagg ccggctcgcc gttcggcagc gtgatctgga agaacccgtt gccctcgatg
     3601 accagatcca gcggctggcc ggtgttctga aacgacccct gcgtgaacag cttctggatc
     3661 gatgagacgc gcgagccgtg gccgacattg atgccggtgg gtatttcggt gttgatgctg
     3721 gcgttggtgc cgggcgactg caccgcctcg tacagcaggt cctgaaaatc gacccggccc
     3781 cgcttgaacc cggtcgtgct gacgttcgcc aggttgttgg cgatattgtc gatgttcaac
     3841 tgctgcgtgt gcatgccggt ggcggcgacg tatagggctc tcatctcagg gcactctcct
     3901 ggcgggggtg ttcaaactat tcgcgcgcgg cgcggatccg ttgctcggtc agctcgtcgg
     3961 ccgtctgcag cgccttctgc tgggtttcat aaatccgttg ggcctggatc atccggacca
     4021 tttcctcgat cgggttgaca ttggagccct ccaggtatcc ctggcggatg cggccggtgc
     4081 tttcacccgg ttcgaccccc ggcggcgcca tcagcagccc ttcgccccac ttccgcaaca
     4141 cgctcgggtc gctgaagctc accatcccga gccgtccgac ctgctggccg ccgacgaaaa
     4201 tctcacccgt ttcggtaatt tccggcgcgc ccagcccagg atcgatcgtg atcggctcgc
     4261 ccgagtcgcc gaggatccgc cgccccgcgg ccgtcgtcag ttctcccgcc gcattgagcc
     4321 ggaaattccc cgcccgcgtg tagcggggag cttgctgtcc ctccacctcg accataaaat
     4381 agccgtcgcc ctcgatcgcg agatccagcg gctgctgcgt gtgctggatc gggccggcgc
     4441 tcagatccgt gaaggcggtg ccggccgtcg gccaggcggc ggcagatttt gccgggtcgg
     4501 cgccgccgct cggcgcggtc aggccggcct gcagcgcacc gccggcgagg tagttcagga
     4561 acaccgcgcg gtctttcttg aagccgggcg tgctcacgtt ggccagatta ttggcaatcc
     4621 ggtccagcgc gcgctcctgc ccggtggcgg cggcgatcgt caggtggatt tccttcatcc
     4681 gctcctcctc cgaggcgtgg ccctgccggt cggcgggtgg tgcaagagtg ataccatgat
     4741 ccacccggga ctcgggttcg cccggatttt gtggtctata ttattgaaaa tgaatggttt
     4801 atagcatttc gcgagggcgc tcgggcggca tccgaaggcc cggcaaaacc ttccgcttga
     4861 cggttccttg acgccttcgc cgctgaaggc ttggcatccg cgtcagttcg ttggcgatta
     4921 tgacaccttc cccaattccc ctcggcatcg gcatcgacgg cggcgccacc gctttcaaat
     4981 ggaccgtcgg cgcccccggc ggcgatctcc ggcgggggac cgcccccggc ggcaacccgc
     5041 acgtgatggg ctgggagccc tggctcgccg ctctgggcga cgcgattgat ggcgccctgc
     5101 gcgcggccgg agggcaacgc ggcgaactgg gcgccctcgg cctcggcatg gccggcgtcg
     5161 atcgcccggg cgagcccgaa cgaatccagg cctggttgcg tcggcatttc cccaacctga
     5221 accggatctg gatcggcaac gatgcgctca ctgccctgcg ggccggcgcc ggcaagctcg
     5281 aagggttggt cctgctggcc ggaaccggtt cgttctgcct gggaatcgcc cgcggcggcc
     5341 ggcgggcccg cgccggcggt tgggggcatg tcctgggcga cgaggggagc ggctggtgga
     5401 tcggccgcga ggcgctggcg gcgctctgcc gcatggccga cggacgcgaa gcccccaccg
     5461 ccttgctgcc ggccctgctc agtcggctgg atttgaagga cgcgagcgag ctgatcccct
     5521 ggctggccgc gcgcgatgcc gccgctcaga aacgcggcat cgcggccctc gcccccgccg
     5581 tgctggcggc cgcgcgcacg ggcgacgccg cggcggagcg gatcgtcgtc gatgccgccg
     5641 gccgcctcgc cgcgctcgtc cgggccgtcg cgaacgccct ggccggggag ttcgacccgc
     5701 ccgtcccggt catctgctcc ggcgggctgt tcaaggatga agcatttctt gcgcgggtcg
     5761 ccgggcattg cgggccgggt ttcgcctgcc gcgccgcgac ggcccccgcc gccgacggcg
     5821 cgctcgccct cgcctgggag tcccgcgccc cgcctgcggc ggactgaagc acccagcgac
     5881 actctgtcgc acgcggcggt gtctcagcgc tcgccgtccg ctttcccccg cgcctgcccg
     5941 cggggacgcg ccgtctgcat ccgcgcgcgg ccgcaccgcg ggcagatcca cttgcgctgc
     6001 ttatgaaaaa cgcggcgcgc ttccttcatc ttgacccggc agtggcggca gaccatcgtt
     6061 ccattcccac gcgcgaactg gcgcccggct ggcttcccgt ggcatgcttc ttgtatcgaa
     6121 tgcgctgggg aactttgggc gctccgatgc atccatctta gcaggttccg ccgccggtcg
     6181 accagttccc cgcgtccggc gccgtttcca agacccccac gggagttgcg ccagccatgg
     6241 ccatgattcg caccgccgaa ccccgcgccc gcgtccgcgc gctgattcag ttgcagttcc
     6301 tgctggccgc gctgaccggc ctgagcggct gcacgacgtt tcgcgtcgcc tccgagagca
     6361 tgttcatgat ctcgcccgag gaagagcgcc agatcggcca gcaggtgaag tccgaaatcg
     6421 acgggcaggt gcagttcgtc aatgattcgc tcgtcgtgaa ttacgtccgc gatgtcggcc
     6481 ggacgctcgt cgcgcacgcc cccgagagcg cgttcgatcc gcaattcttc gtcatcgcca
     6541 atcccgagat caacgccttc gccatccccg ccggctcgat ctatgtgcac accggcctga
     6601 tctccgccgc cgacgacgaa gccgaaatgg ccgccgtcat cgcccacgaa atgggccacg
     6661 tgatccgccg ccacagcgcg cgcggcctcg cgcgcaacac cggcgcgcag ctcgccggcc
     6721 agctcctgct cggcgaaaac tacggcagca tggcgcagtt gctcagcagc gtcggcctgc
     6781 ttaactacac ccgacaggac gaggcggagg ccgaccgcat cgccgtggcc acactgcacc
     6841 gggccggtta cgaccccgac gcgctggcca ccttcttcgc caaactgaag gaacgctatg
     6901 gcgatcgcgg acagatcgcg acgttcttcg cctcccatcc gcccacgacc gagcggatca
     6961 acagtgtccg cgccatggtg gatcagctcc ccgaagcgga gtatcgccga ccgacgagcg
     7021 acctgcgccg cgtccaaaag cgcctggagg aactcggctt cgccgcggag tgactctccg
     7081 gccgatgcct gggagcgcca ctctcctgag tggcgacatc cccaacggcc cgcccagaaa
     7141 ctcggctctc ggctctcgag aatttggcca ttgccactcg tctgaatctc cggtttgtaa
     7201 ccaccgcccc cctcacctgc cgctggacct gatcttgcta ctccccatca gcaccagcgg
     7261 ccgcccgtcc cgccggcggg gttggcggcg gccatcccct cgggtgagtg aggccacatg
     7321 gtcccgtccc ttacgtcgct caagccggcc cacctgaaac gttatgccga aatcgcccgc
     7381 ctcctgctgc gctatggccg ctccgacctg gtgcgccaga gcggtctcta tcaactcgtc
     7441 gccgaggaac agcaacgcga cctggccgcc gcgccggccg aagcgcgcga gctggccaac
     7501 gacctcgaac gcttggggcc caccttcatc aaactgggcc aggtcctctc cagccgcgcc
     7561 gacctgctcc ccttcgaata catggaggcg ctcagccgcc tgcaggataa cgtcaaaccc
     7621 tttccctacg aggaggtcga gcggatcgtc gaagacgagc tgaacgtctc gatccataag
     7681 gcgttccagg agttcaacgt ccggcccgcg gcggcggcgt cgctcggcca ggtccaccat
     7741 gccgtgttgc gcagcggcca gccggtcgcc gtcaagatcc agcgccccgg cgtccgcgaa
     7801 caggtgaccg aggatctcga cgcattgcgc cggatcgccg agacgctcga cgcgcacacc
     7861 gaactgggtc gcaaatatga attcaccctc ctgctggacg acctgcgccg cctgctcatc
     7921 cgcgaactga actaccgcga ggaagcgagc cacctgcgca tcctggccga tgggctctgc
     7981 gatttcccat cgatcgtcgt cccgcgcccg atcgacgact acaccacctc gcgcgtcctc
     8041 acgatgcagt atgtgcgcgg ctacaaggtc acctccatca gcccgctggt gcggctcgag
     8101 atcgacggcc atcacctgct cgaggaactc ttccgcgcct acctgcacca gatcatcgtc
     8161 atcggcctgt tccatgccga cccgcacccg ggcaacatct tcctcaccag cgaccaccgg
     8221 ctggcgctgc tcgacctggg catggtcggc cgcgtccccg ccaaccgcca ggatcaggtg
     8281 ctgcggctgc tgctctcgat cagccagggc ctgggcgagg agaccgccga aatcctgctc
     8341 gaaatgagcg agaagcgcga aaacaacaat gtcgaggggt tcatgcaggg catcgaggat
     8401 ctgatctccg acgtcgccgg cggcgcgttg ggcgacatga atatcggccg ctcgatggtg
     8461 cagcttcagc gcgtcgccgc cgacaacggc gtgcggctgc cgcgcgaggt gcggatgatc
     8521 ggcaaggccc tgctcaacct ggagcgcgtc ggtgcggtgc tctgcgcgga tttcgatctg
     8581 cgcgccgaaa tccgcgacca cacggccggc gtcatgcgcc acaagctgct cgaggcggtc
     8641 gctcccggca atctgctcaa gagtttcgcc gacatcaaac gcttcgtgct caagctgccc
     8701 gccgaactga atcagattct cagcgtcgtg acgcgcaacg gtttgcgcgt gcgcgtcgac
     8761 gcgatcgacg aagaccggct gatggcaggt ttccagaagg tcgcgaatcg gattaccgtg
     8821 gggctggtcg tcgcggcgat gatcatcggc gccgcgctgc tgatgccgat ccagaccggc
     8881 ttcgtcctgt tcggctatcc cggcctggcg attctgctct tcctgttcgc cgcgctgatc
     8941 ggaatctaca tggtgatcag catcctgtgg aaggacatca aggaaccgcg ccgcccgcgc
     9001 cggcgctgac ggcgccgggg caggggagag cgcggagccg gcgcgccgac ggtcagcgcc
     9061 gcagcttgag cgaggagagc ccgtggcgcg cgccgcggtg gcgcgggttg atcttcaaca
     9121 ccttctgata ttcctgaaac gccgagcgct cgtccccctt gagctgatac gtcagcccga
     9181 tctcgtagtg tacttcatcg ttttccggca ggtagcgcgt cagctccttg aagtggacga
     9241 ggcattgcgt gtagagcccc ttgtcgcgca gcgtccggcc gatgctcata ttcgccttga
     9301 tcagcgactc ggtgatctgc ggattcttcg ggtccatcgt gcgcgcccgg cgccagacca
     9361 tgaacgcttc cttgatccgg ccgctgttga gcatcgccca gcccagcagg ttgagcacct
     9421 cgatgttgcg cggaaaccgc cgctgcatct cctccagttc cttcgtcgcc tcttcccact
     9481 tctgctcgtt gtagagcagc gatgccagcc ggaagtggct ttcgaagtca ttgggattga
     9541 gccgcaggat ttcgcgcaga tcctcgaccg aatgggcggc gcgcggcgcg gacggctcct
     9601 gcgttcccct ggcctgcgcc ggagcgcctc cctcggcctt ctcctcggcg ggcgcttcgc
     9661 cgctttgccc tttctccttc aacgcttcca ccaggcacgc cttcaggtac tcgttgtctg
     9721 ggtcctggtt cagttgcgcg cgccagcgct tgatcacttc ctgccagtag cgccgcgctt
     9781 cctcgacgcg ccccgcgtgc tcgcacgcca gcgccaggtt gtggatgatc tcggttccgt
     9841 cgggattcat cgtggcggcg cgcttgagca cgtcgaccgc ctcgtcatac agaccgtggc
     9901 tggcgtagga gtagcccagc gtgatgtagc tgtagagcag attgttcttg gcgcgccggt
     9961 gcgccgggtc cagttccagc acccgctgcc agtcctcgat cgcctcggcc cacagcttct
    10021 tctgcacgcg cttgtacgag cgcatcatca gcccctggat cagcttgggc gtggcggccg
    10081 cgcggtccac ctggccgctg ttgatcttct cctccagctg gcgcaggccc tggtcgatct
    10141 gcgcgtcggc cagttgcgtc ttctcctcgg cgctgaagaa gaattccccg cggatgtagt
    10201 tgaacgcccg cacgtcctcc cgcgctcgcg acgcgggcgt cttgagccga tcgaacgcgt
    10261 tctgcacgat catgaagcga tcggtatggc gttccggatc gtacttcttg accagttgga
    10321 cgtaagcctt cttgatgtcg tccaggctgg ctcccttgtg cagcccaagg acggcatagg
    10381 cgcttttatg attatctgcc acgtcgaatg ttgaaggccc agattgactt gccgcggacg
    10441 ctgcccaccc ccgcctcggc gcgcgggggg tcccacttcc tcacttcaaa ccatgggcgc
    10501 caccatggat catattgttg tagcggaaag gtgcaaggag tttcaaacca aaaccttgac
    10561 gccccaaatg acgcttgccc ttcacgttct acagctcgcc ttttgcggat tcgctctcgc
    10621 gaaaattgca gaattggcgc aattcgcgct tttctgctcg gccctgctcc ggtttttcct
    10681 gccctccgga tgcggcctct aatacaccgt cgccacctcg tgcgcgagcg ccgcgccggc
    10741 cgaatcctgc gcgctcgccc cgcctccatg ttccggccac atcgcatcct tgcgcgcctg
    10801 cgtctccgcc agcaaccggc gcagctcggc ccgatcctcc tcggtcagca ggtgattgtg
    10861 cgtcaccacc cgctgcgcct cgttcagcag cgactgcacc cagaacggcg tccgctcgcg
    10921 cagatacagg cagccctggc attcggtccg cacgcgcccc tcgcgcagct tgcgccgcgc
    10981 cgcccgcatg ttcgggccgt tgaacgccgc cgccggcccc tcgccccaca cgtcctcgat
    11041 gtcttcctcg atgtagcagc acatcttcat ccgcccgttg cgctcgatcg tcatgtcgcc
    11101 gttgagctgc acgcagccgt aggccgacgt ctggtcatac agctcgccgc gataacccac
    11161 cgccttctgc cgccagaatg aaaagcccag cccgagccgg tcggccagca ccttggcgcg
    11221 ctgcagcatc cgctcgatga tcggcgtgtc cagcacgctg atgtcctcat cctccggctt
    11281 gtaggccacc gcgctcgagt acgacaccga ctggcagccc atgcggtgcg ccagccgcgc
    11341 catcgccggc gcctgccgca cgttgtattt cgaaagggtc atgttcacgt tgagccgcgg
    11401 ccggtccacg ccccgttccc gcttcaacgc cgcgagcgcc gtcacctgct ccaccaccgt
    11461 gtcgaatttc gccccggcgc gcagccgctc gaacaccgcg cgcgtcgtcc cgtccatcga
    11521 gatgtgcagg tcgtccagcc cgaccgcgac aagccgctcg cgcacctcgg gcgtcaggct
    11581 catcccgtgc gtcgacgtcg cgacgctgat ccgccgcgcc ttgcacgcct cgacaaactc
    11641 aaaaaaccgc ggatgcagga acggctcgcc cagaccgaac aacgacacgc acgccgcctc
    11701 ctccagctcg ggcagcttgc gctcgaattc cccgaagtcc atgaacagat tttcgcggat
    11761 ccccatcgat tcgcgcaggc acagcgggca cttcagattg cacgtcgtcg agaccgccgt
    11821 ctgcaccttc gtcggccgcg cccgcatctc gtcgtccaga aacgtcagat cgaccagatg
    11881 gcggacgtgg ttgatgacct gctcgcgcgt attcatccat ccagtttacc gtctttcccc
    11941 cctccccaca accccctgcc ctcatcatgc aatacatcgt ccctcgtcct cggccctcga
    12001 tcctttcttc ccccgcccct gtctcccccc cgttcacagc ccctccgtcg tctcccgctc
    12061 aaccacaacg cccatctcgg gttccacccc ggatcggttc tccagcctga actcccccgc
    12121 gaccagcccc gtcgtcagtc gcatcccggc ggcgccttcc tccagcagca cgtggcgttc
    12181 ggtgggcgcc tgcacataga tgctcttcag cgtcagcctc ccgcccgggt ccgtcccccc
    12241 ggcgtccccg gccacgcgcg ccatcgtctc gccggggacg acgaaatgcg cctgccgcac
    12301 ctgaacttcc ccgccggcga cgtcgatccc gaatttcggc gcggcccaga agagcgaatt
    12361 gaaaaagcgg atcggtccgg cgaccgcgtc gggcgccgcc ggcttgacgg cgatatacgc
    12421 ttcctcgcgc gccgcaaaca ccgccgcctg gaagttgatg aactcaagac ctcgcgcgcc
    12481 gaccgcttcg atccgcaccc cctgggcggt tccgtcgctg ccgtggccga tcacgaccgt
    12541 ctgcgcccgc ggtcgttcgg gaaccgatac ttctcccgag atcgaactgc ccgcggcgac
    12601 cggaatcgcc ggttgcggct cggtaaagcg cagcccatgc cgcgaaccgt aaacgaaatt
    12661 attgaccagc cattgattgt cggcccccgc cagcagcagc gcatccagtt gcgccttctg
    12721 ctggttccac cacacctgcc attccctccg gccctcatcg ggatgccccg ggtaaggcga
    12781 gcgcgcgtag taatgcgggt tgaactgcgt gttgctgacc cagcccccct tcgattcgcc
    12841 gatgaaaatg ccggtcctca gcggcgcccc ggcggcgtag tcgatgacat gccccgtgca
    12901 gtcgtgcgtg ccgaaatcga tcccccgcca ggcattgctc agcgtcacgt tcaacgcata
    12961 cacgccgtgg ccctggccgc gcaccgccca cgggtaggcg accggctcgc ggaaatcctg
    13021 ttcgggataa tagaccgtca acccgcgcag gcccgccccc ggccgcaggt tcagcagcgg
    13081 cgtgccctcg gcttcgccgc ggccggccac cgccatcagc accgtccctt cgcccatcgt
    13141 atgatgcggc acttcccaga tgccgcgcag ttcgacgccc ggcgggatcg tcagcggacc
    13201 ctccagccga tagcgcccgg ccggcagata gaccgtgccg cccttctccc cggcggcgtc
    13261 gagcgccgcc tggatcgccg ccgccgcgtc ggactcgcca tcggcggggg cgctgaacgg
    13321 agccgccgtc gcgacgaaga cgtcgctgga gccgggacgc ggaaattcag gccagggaac
    13381 cttgacgaca cggtcgccaa cgaagcgatc ctcttcccag tcaaacgcgc catggatcac
    13441 aaaaccggga ttgcccgaca cgatcttcgg cagtcgcgaa tcctcactat tggcaagaaa
    13501 acggtccttc catcttcggg cactccgctc gtatgcccgg ccggacaagt cggccggtat
    13561 cccgttgccg gccaggatcg ccgatcgcat ggtctcggcc agatgaatcg gcgcggttgt
    13621 tgaaaacgcg catgcttcca cggtcagatg cccggctctg gcctcaatgg atgggcaatc
    13681 ggcggcggta agcgaacaat tctgcagcga cacgaccccg tcgccttcaa gcacaacggc
    13741 cgcctcgcca gccttgcagg cgatgcgcgt cccgttcagt tgcaggattc ccgtgaaatc
    13801 cggcgccgtg cgcagcgccg ccgctcccgg cccgctctcg gcctccaggt ggctgcccgt
    13861 catcgcgaag ccgatataat tggtttgcac ggcgttgagg gcggtgtggc agtcgctgat
    13921 ccagacattg aaggcctggg cgttggacgc gccgttcggt ccgtcgcgca gcgtcaggcc
    13981 gttgcggtag ccgtcgatcg cgacatcggt gaaatactgc cagtccgacc gctgcatgac
    14041 gatcgcatcg gcgtgggttt tcaggtgacg gcgcagcgcc ccattgcgcg aaaacagccc
    14101 cccggcgagc agcccgctct tctcccacac ccccggcgag atcacaaccc gctccagccg
    14161 gccgatgtcg gtcgtggcat cgatcaagac tcccgtcttg agcgctgtca tccgcacgtt
    14221 gcgcagaaaa tgcagttcat tcggctgcgg cccgatcttc accccctgcc acgggttcac
    14281 cagcgtcacg tcgcgcaccg tgatgctgtc gacgcccgtc tgctcgatcg tccacggata
    14341 cgccgccggc gcgcccgctt cctgctccgg atagtaaatc gaaagcccga ccaggccggt
    14401 gcacagctcc atcgtgatga acgcggggcc cgcctcgctc cctcggccgc cggtcgcgag
    14461 cagcagcgtc ccctcgttca cgcgggccgg atcgtattcg ccccgcagcg tgacgccccg
    14521 gggcagggtc agccggcctt cgatgagata gcgcgcggcg ggtgcaaaga cgaccgcgcc
    14581 gccgacccgc cccgccgcat cgaacgcctc ctgaaacgcg cgcgtatcgt cgatccgccc
    14641 gtcgccgatc gccccgtaat cgcgcacgtt gaacccggcg atgatcggct cgctggtcgc
    14701 gaacttcgtc gggtagagcc gcgggcgccc ttcggccgtg gcgaccatct cgcgcgcgat
    14761 cgacgcctgc aacgcgtcca tctccgcctc cgacggcatg ccgtagcgcg cgcacgcccc
    14821 cacgatcgcc gccgcgaaca gcatcccgcc gaaccagatc cgccgccgta acgcctgcat
    14881 cggtccttct cccaatgaag agtcaaacca cggagcatcc agggatcccg ccgcggccca
    14941 gccgcaaccc gccgaccttg cccgctaacc cgccaagatg caaagaagaa atcggtatcc
    15001 gtatcgtcat cgcaatcgcc agcggtcccg gcctcgtcgc acccccgcat ccatcggaat
    15061 gccagattcg acaactcaag aactccctca ctcctccccg ttctcctccc ccggctccca
    15121 cgaatccacc gaatacaaca ccagccgcga gtcctcgtgc cccgggttgt tcaacgaacg
    15181 cagcagcttc aatcccgccg tcgtctccgc cgtcggccgc accaggtagc ccaactccgg
    15241 ccgcttcgtc agcacctggt cctcctcgat cagcagatac cgcgcgttga cttcatccag
    15301 gaacgccagc agatcgccgt ggtttgtcac atacggcagc gcctcgcgcg gccagttcag
    15361 ccggaagttg aacttgttct tgcgcgacac caccgtcccc ggcgccagcc ccgcctcgcg
    15421 cgcccattgt gccagttggt catagtgccg gtcctcgtca tccggcttgt cgaacatgtc
    15481 ctcgggcgcc gtctcctgcg ccatgccgac atataacgcc agcgccagca cgtaaacggc
    15541 gcgccgtttc caccgtccct ccctcagccg ccggccccgc tcaacaagcc acaagacccc
    15601 cgccgccagc agcggcggcg cgatcagcac cgccggcacg agcacatacc ggtcttccac
    15661 atgaaacggc agcaggatcg ccagccccgg caggaacacc cccagcgccg ccagttcccc
    15721 ccgccgcacc gccgccgggc cgcacgccag cgccagcgcc cccagcgcca gcagcggcca
    15781 gccccatgcc aggccgatga agttattgag cgcctcgacg atctctcccg tgttgaccca
    15841 cacccggccg gccagctcgc gtggatagtg cacgagcatc ccccacagcc cctcctcggc
    15901 catccgctcg gcccgccgcc gcgcctccgg ttcctcctgc tcttcctccc atccgccata
    15961 gatgatctgc tcgtaggcca gccggtcctc cgtgtacgtg acgccgtgga gcagattctt
    16021 ctcggtcttg gccgttagcg tccactcgcc cgtcgccgcg cgcaggtaga gcagataagg
    16081 cgacgcggcg acgatcgcca caatcagccc cgccgccgcc gcggccgcgg gaaacggccg
    16141 gcgcaggccg cgcagccgca caagaaacca gacggcggcc agtccggaat acaccagccc
    16201 atcgggccgc gcgacatagg ccgccgccgc gcccagcccc gccacgaccg cccagaccag
    16261 ccgcatccgc cggcccgttg ctccatcggc cagtcgcgtc agggccagca gctccgcccc
    16321 cagcgccagt agcagcagac cctcgggacc cagccgcagc agcgcccccg tccagaaccg
    16381 gttggtgaac agaatgaaca gcgccagcgc cggaaaccag cgatcgccgc cggcgccccc
    16441 gctccaggcg taaacaaatg cgatccagac cgcgatcgcc ccggccgcaa tcgccgcgct
    16501 ggcggcgaac gccgacccga aaatgcgccc cgtgaccatg taaatcggcg tgacgcacag
    16561 cgggtagagc ggcgagaaca gctccaggct ctccccgttg ttcttcgtga accccgcccc
    16621 gcgcgcgagg ttgcgcgccc cctccaggta gtgcagctca tcgatgtcga aactcaggtc
    16681 gtgcagccgc ccgacgccat acaccagcag gccgatgaca aacagcgcga cgcgcagccg
    16741 ccggccggat gcgcctccag cgagccgccc tccggcggcc gtctcgtctg gatggggatc
    16801 aggtggatgc tggaccatga caacgcgtcc caggcaggcg gcaagccgac cgcgcgattc
    16861 cacgccgccc cgcccaccgc cggtccccca ctataaattc cacccctcct tctgcacgct
    16921 ttaaattcca tcaccccccc gtcccccgct catcgtccct cgtcctcgcc cccaacggcc
    16981 cacctcaaaa ttcggctctc ggctctcgtc cctcgtccct cgtcctcggc tctcgaaaat
    17041 ccatcaccgc ctgcgaccgc cggcatccct gccggcatgc ttccccctct tcccaccctg
    17101 ccccccatcc tgctacaatc caacccatcc tcccgcccgc cggctcgccc acttcgccca
    17161 gggaggttcc cagcatgcga tctttcctgc cgcttgtcgt cctggcagcg ttcagcctgc
    17221 cgctgccggc ctgcgcccag gaggcccccg cccctccgtc cccaaccgat ccggccggcc
    17281 cggtggaaat cctcctccac gccgaccccc cgacgaccgg cgccgtcgcg cgcccgcaac
    17341 tcccccccga tgaattcacc gaccaacgcg cctggatggt cgagcaccag atccgcgcgc
    17401 gcggcgtcac cgacgaaacc atcctggccg ccatgcgccg cgtcccgcgc caccagtttg
    17461 tccgtccgga cgacctcgcc cgcgcctacg gcgatcaccc catccccatc ggccacaacc
    17521 agacgatctc ccagcccttc atcgtcgcca ccatgaccga gatgctccaa ctgactcccg
    17581 aaagtcgcgt cctcgaggtc ggctcgggtt cgggctacca ggccgccatc gccgccgaac
    17641 tggcccgcga ggtcgtcacg atcgagatta tcggcgacct ggcccggctg gcggctcacc
    17701 gcctgcgccg cctcggctac accaatatca ccgtcctgca cggcgacggc tatttcggct
    17761 ggcccgccgg cgccccctac gacgccatca tcgtcaccgc cgccgccgat cacgtcccgc
    17821 ccccgctcct cgcccagctc aagccgggcg gccgcatggc gatccccgtc ggccgcgccg
    17881 gctgggcgca gaacctgctc ctcgtcgaaa aagacgccaa cggtgcggtg agcacccgca
    17941 atctgctccc cgtccagttc gtcccgttga cccgcgagcg gcgttgacgt gtcgcccgac
    18001 cgccccaacc cctcggccgc cgactacctc tcggtcgcgg ccgtctccgc cgccctgctc
    18061 gccttcgaaa tcctgctgct gcgcctcttt tcgctcaccc agtggcacca cttcgcctcg
    18121 ctggccgtcg ccctcgctct gctgggattc ggcgcggccg gtacgctgct gacgctcctc
    18181 gccgaccgcg cccgccgctg gggcgacccg ctccacgccg gcgcgaccgc cgccgccggc
    18241 ctcttgctcc tcgccatcct ccccctcaac cgcctcatcg agatccaccc gctgttcatc
    18301 atctgggacg tccgccaact ccttcggctg ctcgccctgg atctggccgc ctttctgccg
    18361 ttcatggccg gcgccgtcgc gatcggtcag gtcttcgtcc gctggcccgc cgccacgcgc
    18421 cggctctacg gcgccaatct gctcggctcg ggcgcgggca gcctgggcgc cgtcgctctg
    18481 ctggccaccg tcggtttgcc ggccgccctg atgctgctag gcgggttcct cctgctcacg
    18541 gccgcccttt tcggctgtct gccgcgcccc ggcgcccgtc cgcgcgcccg tgcctgcggc
    18601 gggctggccg cggccggcct gctccttctc gtggcatggg ccgcgctccc tggctccccc
    18661 gccatccgcg tcccccagtt caccgaactg aatttccggc tctcgctccc cgatgccgaa
    18721 gtgatcgccc agcatcacgg cccgcgcggc ctgagcaccc tgctccggtc cagcagcaat
    18781 cggctcgccc ccggcctcag cctgcagtgg ttcagcgtcg tcccgtcgca cgacgtcctg
    18841 attctggacg ccgatcgcgc catccccctg ccggcgcccg cggcgttcgg cgacctggac
    18901 tacctcgatg ccgcgctggg cgccttgccg tggcgcctgc ggccgcgcgc cacaaccctc
    18961 gtcctcggca cgagcgcctg gctgatgccc ctccacgccc gccgggcgga cgccgctgat
    19021 ctcgtctggg tcgaggacga cccgcgcatc tcttccgccg tcgcgcggac cgaccccggg
    19081 ctggcggccc gtctccgccg ccaggcgccg cgctcggccc tcgccacttt gcgcaagcag
    19141 ttctcgctca ttgtcgtcga gaccgccgcc ggcggcggcg aagccctcgc cgaatccccc
    19201 ctcctgacgg tcgacgccct cgccgcgatg agccgccgcc ttgcccccga cggcttcctg
    19261 gtcatcccgc tcccgctggc gaaccccccg cgccatctcc cccgcctgct ggcgatgatc
    19321 caccaatgcc tggttcaagg gggcgccgac gatccgccgc gccatgtcat cgccctgcgc
    19381 gcgcttcacg acgcccttgt gctcgttgcc cccgccccct tctcccccgg cgacatcgcc
    19441 cgcgcgcgcg ccttcgccga ccagtggggc ttcgacctca tctggcacga cggcctcgac
    19501 cccgccgacg ccaatcgcca tgcccggctc gccgaacccc tctactacgc cgccgccgcc
    19561 gcgctgctcg ccggtcgcga cacgccgacc cccgccgccc gcttctacaa ccgcctcccc
    19621 gccaccgacg cccgtcccta tttctggtat tccgtcgatt ggacgcggct gcccgatctg
    19681 ctccggacgc tcggccggcg cggcctgacc ttcgtcgatt gggacgcgct gatgcaggcg
    19741 accgccctgg ccgccgctgc cctgctggcg ctgctcctca ttgtcgcacc gctgggccgg
    19801 ctgccggcgg cgcgacgacc gctgggccac gcctcgatct ggctttattt ctccgcgctg
    19861 gggctcggct atctcatgct cgaactggcc gtctttcagc gggccaccct cctgttgggc
    19921 agccccgtcg tcgccgcggc cgtcatcttc gccaccttcc tgatcgcgtc gggcatgggc
    19981 agcctgaccg ctccggaagc cgccatcccc tctatcgccg cccgcctgtt cgccttcctc
    20041 gcgctcggcg tcgtcctcgc cgccctcgtt ctctggggcg ccggcgaccg cctgctcgcc
    20101 ctccgcgcac tgccgcgcgc cgtcgttctc gtcttcgccg ccgcgccgat ggcctgggcc
    20161 atggggcgcc cgatgccatg gggactccgc cggctcggcg cccaaccgga ctggatcccc
    20221 tgggcctggg gcatcaacgg cttcacgtcg gtcctggccg ccccgctggc cgcgctcgtc
    20281 tccgtccgct ggggtcagcc cgtcacctgg accgccgccc tcctcggcta cgccgtcgcc
    20341 gccgccgtcg ccttccactg gtcccgcccc cacccctgaa cgtcgtccca catccctcgt
    20401 ctctcgtcct cgcccccaat ggccttcccc aatgttcggc tttcggctcg tgcctcatcc
    20461 ccggctctcg aaaaccctcc atttacatcc tccgctccga accgccgatt actccgcaac
    20521 ctctccgaaa aactccgcgc tcatcgcgca agagcccgtg cagcggacgg cgggaataaa
    20581 gcgcagggtg gaggccgcag gccggaaccc ctgaaaggtg atcggaatcc gcgcgccccc
    20641 cgccaacgcc cccgcattct cacgcgctct tccgcctggg cttataaccc agcgcatggc
    20701 acacccgccc cgcgagatac ccgaccgcgc aggttgcatc gtaaatcagc agcagcaaca
    20761 ggctccccag cgcaaaccgg atccccttct tctcctgtaa aaaagcgagg atcggcgcat
    20821 gcagcatcac cgcgaacaaa gcacaaccgc tcgccggcag catcagcggc aatcccaccc
    20881 cgcgcgcgaa gaccgccgcc gcacaggccg ccgctcccgc cgccagcccg gccgcgacgc
    20941 ttgccaccgc ccccttctcg cgccagccca ggttcaattc cccccggccc agctccggat
    21001 accgctgcag cagcaccgtc cacgggatcg cccgcccgaa cagatcggtc cgcacgatcg
    21061 acccgagcgt ccaccgcttg tggtggcgca ccagaaggtc atggcgcaac gcgatccgcc
    21121 cgcccccgcg ccgcagccgg tagccgaact ccatgtcggc gatcggcgcc cccggccaga
    21181 aggaatcgtc aaacccgccc agccgctcga acgccgcgcg ccgcaccgcg ccgcatcccg
    21241 tccagaatgt cgaaaccgat ggcccctggc gctggtgcac gtagtgatgc agcaggttgc
    21301 gatactgcga gcagaacccc gccgccgccg gccggtcgtc atacgaacca atgaccgcat
    21361 ccacgtcggc gcgatccgtc aggaactcgg caacgatccg cgcgctcccc ggcgccgccg
    21421 tcacgtccgc atccagaaac cagaggattt ccccggtcgc cgcccgcgcc ccggcgttgc
    21481 gcgccgcgca gtcccccccg ttccgctcca gcgcgagcag ccggacccct tccgcgcccg
    21541 cggcgatctg gcgcgagccg tccgtcgagg cgtcgtccac gacgatcacc tcggcgatcg
    21601 gcgcgccgct ccgccgctcg cgcgccaccg ccgccagaca ctccccgagc gtccccgccg
    21661 cgttataaca gggaataatg acgctgatcc gcatcaatca ctcacgccct cgctcgccct
    21721 cgtcctcgtc cccaacggcc ctccccaaaa ttcggctctc ggctcccgtc cctcgtcccc
    21781 ggctctcgaa atgcttcttc aaccaccctc cgccccccac ctcccgctgg cggattcccc
    21841 cgcgcccgtg tattgatgat ccactccacg cgcgccgctc cgtccccccg tcggcctgcc
    21901 gcggttggag accttgccca cggttccacc cgatttgacc gccatcaaac cagacctggc
    21961 aagccactgg agcggtccgc cgcgcgccga ctggcgcctc cccgcctcgt ggcatcaata
    22021 ctccgccgag ctccacggcc ggctcctcca gcgctggcgg ggcattctcc ccccgggcac
    22081 gccgattctc aaaaccgatc ttttcgagga ggcgcaggcc gacgccaccc ccgccgcccg
    22141 catcgccgac ctcggctggc gcccgctcgg gatggatctg ggcgccggca tcgcccgcgc
    22201 cgcccgcgcc cgcgcgcccc aactggccgc cgtcgtcgtc gccgacgccc gccgccttcc
    22261 gttccgcacg gccagcctcc ccgccatctt ctccaactcc accctggatc actttcataa
    22321 cctcgccgac ctcgacgccg ccctggccga actgcaccgc gtgctggccc ccggcgggcg
    22381 cctcctgctg acgctggaca acccctgcaa cccgctcgtt ggcctgcgca acgccctccc
    22441 cgccgccctg accgaccgct tgcgcatgac gcgctattac gtcggtcgca cgctcggccc
    22501 gcgcgccgcc cgcgcccgcc tgcttgccct cgggttccag atcgaagccg ccggcaccct
    22561 gatgcacgcc gtacgctacc tcgccctccg cctgctccgc cgccgcgaac gcgccggcgc
    22621 cgacgccgcc cccctcctgc gccgcatgct cgccatggaa cgtctcgcgc gctggccctc
    22681 cgcgccgctc accggccatt tcattttcct cgaggccgtc aagccatgct cgaacgcctg
    22741 agcaacctcc gccatgccgg ccgcatgctg gccgcgctcc cccgcgaggc gcgcttcccg
    22801 ttccgcccgc gcgcggaaat cgaagcccgc cgcgacgccc gactgcgcga aacgattcgc
    22861 ctcgcctgcc gcgccgtccc ctactaccgc gagcggatgg cggccctggg cctgacgccg
    22921 gaggagttcc gctcgatcga cgatctggcc cgcctgcccg tgaccccgat cgacgatctg
    22981 cgcgacgcgc cccgcgagtt cctctccgcc cgcgtcaacc ccgaccgctg ccaactcctg
    23041 cgcagcggcg gcaccaccgg caacgaaaaa tgggtctggc acgaccccgc ctcgctgctg
    23101 atgaacgtcg ccttcagcga gcgccaggaa ttcgtcatcg cccgcttcgt cggccatcgg
    23161 ctccgtcagc gccagctcgt catcaattcc acttcgagcg tctccagcac cgtccgcgcc
    23221 gcctaccggc gccgtctggc gctgctgcga cccctgggac cccgccgcgt gcgcatcggc
    23281 atcatggagc ccttcatgga tcaggtcgcg gcgctcaacc gcctccggcc ggaggtcctc
    23341 atcaccaacg gcctgttcat cggcgatttc tacgaacgcc tgcaacgggc gggcctcgcg
    23401 ccccaccggc cccgcgtgat tgtcgccggc gccgagaccc tccccgccga tcagcgccgc
    23461 cgcatcgagc acgacttcgg cgtccccgtc atcatgacct accagtccac cgagtcgctc
    23521 aagatcggct tcgaatgcga gtatcgccgc ggttatcacc tccacgagga cctctgcgtc
    23581 gtgcgcctgc tcgatgccga cggccggccc gtccccccgg gcgcggtcgg ccgcatccac
    23641 atcaccaacc ttgtcaatcg cgccaccgtc ctgctcaatt tcgatcaggg cgacaccggc
    23701 cgcttcctcg acgaaccctg cccctgcggc cgcaccctgc gccggctcga actggtcggc
    23761 tcgcgccagt ggccgctgct gcgcgcgctc gacggccgca ccctccacgg cgccgatgtc
    23821 atccgcccgg tcaaggaaca taccgcggtc acgcgtgtgc agatcctggt cgagcgcccc
    23881 gacctgtggc gagtgctcta taccgacccg cggaacgaaa cgaccgcggc gtggcgccgc
    23941 gacacggccc gccggctcga acgcgccgtc gccgggcccg gcatcaccgt ccggctcgaa
    24001 cagcgcgacc agctcgag
//
LOCUS       DAOPCO010000034        23887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2279_length_23887_cov_5.249371, whole genome shotgun sequence.
ACCESSION   DAOPCO010000034 DAOPCO010000000
VERSION     DAOPCO010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 23887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23887
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2279_length_23887_cov_5.249371"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLS90_04410"
     CDS             <1..237
                     /locus_tag="PLS90_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01680.1"
                     /translation="ALYEVMVMSDRLRDAVIDGVSTNQLKRLAMAEGMQTLRMAGINK
                     VLEGRTTISEVLSATMADTVQEIAVATDKEDQVA"
     gene            290..1501
                     /locus_tag="PLS90_04415"
     CDS             290..1501
                     /locus_tag="PLS90_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK01681.1"
                     /translation="MPVFQYSARDNRGQIIRGQTEAQNQAAVIRLLREQGLIPTTVEA
                     GATRRAARRAMGKGGKIKLEDLVILTRQFATMIRAGLPLIEVLNILSEQTEKRALKAV
                     MKQVERDVETGSSLNEAMSRHPKVFSTFYLSMIAAGEAAGMLDSILDQIALYLEKVVS
                     IQRKVKSAVMYPSVVSVVAIGITVFLLIKVVPVFGDIFSDMGGKLPAPTRITMGISNF
                     LQDNFLLSVFILIGLALLVWQGGRTPKGRRILDQLKLKMPIFGPLFLKVAVARFTRTL
                     GTLIHSGVNILAALDIVARTAGNVVIEEAVVKTRASIQSGESLAAPLRDSQVFPPMVV
                     RMIDVGERTGALESMLTKIADFYEDQVDAAVSGLTSMIEPLLIVFLGVVVGFIVISMF
                     MPMFKMIELVG"
     gene            1883..3028
                     /gene="dcm"
                     /locus_tag="PLS90_04420"
     CDS             1883..3028
                     /gene="dcm"
                     /locus_tag="PLS90_04420"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HPK01682.1"
                     /translation="MKCYTESFPGNRTFAEFFAGIGLMRLGLEQAGWECHFANDICPD
                     KFAMYKDNFPESDDHFRLGDIHNLKPQDVPTVALATASFPCNDLSLAGMRKGLAGSQS
                     SAFWGFCEILRGLGSRKPPIVLLENVGGFLTSRGGADIREALLALNGLGYVVDAFIID
                     AERFVPQSRVRLFIIGHQFNDGVLDPQRFYESPVRPRLLADFIFSHPEIEWSIRDLPD
                     LPKRQITLRDILEDVDPNSKIWWSEERTNYLVSQMSERHSAVLKVLMGKRAWSYGTVF
                     RRIRNGRSMAELRADGIAGCLRTPRGGSGRQILIKAGYNKVQARLLTPRECARLMGAN
                     EYKISCPLNQALFGFGDAVCVPVVKWIADNYLNPLADRLSLPFAQLA"
     gene            3048..3983
                     /locus_tag="PLS90_04425"
     CDS             3048..3983
                     /locus_tag="PLS90_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4928 family protein"
                     /protein_id="HPK01683.1"
                     /translation="MVKDKLETHLEHFTRENQISGKGPLSLVLILTRDVSRREFPLKA
                     KDFVTGKKGQVKGLSGSKIQTILRDHGITQRLAEEGGRTSRGSIEKMELYVDFLNKLF
                     EKDLLDFERIEMWWIARVKEHFARLPFRVRLDSSKSLRFIFGELLAATVARQRACPGM
                     MITGAVMQHLVGAKLQLLFPDLKIEHHGFSVADSSTMRVGDFKVEDAIIHVTSAPGEP
                     LLRKCADNLSANLRPLIITTETGVGGARALASNLGIEDRLDVLEITQFLTANVYEWSR
                     FSQKEHPASVISLINTYNRIIDECESNPSLRIQVT"
     gene            3989..4408
                     /gene="vsr"
                     /locus_tag="PLS90_04430"
     CDS             3989..4408
                     /gene="vsr"
                     /locus_tag="PLS90_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HPK01684.1"
                     /translation="MDSFTPQERSAIMRLVRSKDTKPERQVRSLLHRLGLRFRLHRGD
                     LPGAPDIVLIRYKTVIFIHGCFWHRHRGCNRASTPSSRREYWLPKFERTVARDAHNRR
                     RLRQLGWRVVTIWECELRHLERLERRLRRLVQVIQSD"
     gene            complement(4710..6206)
                     /gene="rmuC"
                     /locus_tag="PLS90_04435"
     CDS             complement(4710..6206)
                     /gene="rmuC"
                     /locus_tag="PLS90_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPK01685.1"
                     /translation="MEIVLALVTGLLVGAAAAALFLRSRLARAHATAALLGRDLDEAR
                     RRFDELTQRTDQESRLAAELRERVTDLTGRLHAAEAQAERVPRLEDEIRSLGAELRAR
                     AERLAALQTALDDERKAAQEKQALLDEARARLTDSFRSLASEALSKNNEAFLQLATQN
                     LRQHQEAARGDLERRQQAIGELVAPLREQLSRYEKQIQEMETLRAKAHGELLAQVAHL
                     GQTQRQLEQETGKLVQALRAPQVRGRWGEITLRRVVELAGMVDRCDFREQVAIDAADG
                     RLRPDMIVNLPGGRCIVIDAKTPLMAYLEALEAADPDQRRAALQRHASQLHEHMERLA
                     QKAYQAQLRPAPDFVVLFIPGESFLAAAVDQRPDLIERGFERNVILATPSTLISLLKT
                     IAQGWREEQLAENAQRISDLGRELYERLCTLGDHLGKVGSSLEQTVRHYNRAVGSMES
                     RVLVQARRFNELGVHSSKQLKQLQPLDVTPVAPAAEDFLPAPTPSLPD"
     gene            complement(6239..9895)
                     /locus_tag="PLS90_04440"
     CDS             complement(6239..9895)
                     /locus_tag="PLS90_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01686.1"
                     /translation="MTPTSPNRSHSLEDVGLPPARAAADFRQEAYHQAAAAAGLGVWQ
                     WEPRTGRFRASPRCVELLGCSSRAGDDLAELLAPVHPEDRPALSRFIQEGPAEGDSDG
                     FRFRIPRPGDAARWLEARCRRIPATGDGADRGHPLTVGTLLDVTAQVERDHELERLRG
                     DLKERVKESRCLLEVSRLLIDAGGTIAECLAQLPELIRRGWQFPEMCVVRIRVDQASF
                     LSDGWRPPQFTQCVPIALEGEAIGELLVGYPERPPGSSRRIFLPEEDQLARALAERIG
                     EFLHRRRFEDALRQSEERFRSTFEQAAVGLLHADMQGNFLRVNQRFCEIVGYDRDELL
                     RLSFADLIPEGELQRGLAEAECITSGEVTTFAGERRYRRKNGQFFWANLTVSVVEDRI
                     NGQRYMICAMEDISQRKAQEQELLDTRFLMEKTLTSMHEAVLVIDAASGRILTCNPAV
                     ERLFGYPRLELIDQSVEALHLEPDNYRRLRDFLEGSPGQSESLHLECRMRCRDGRVIH
                     TDHTVTRITEEGGWISGVVNVIRDISSRKQAEENLLRREQILEAMAYISERLLRAQQL
                     HEALPSILSRLGETLEADRVQLFETTRNADGSRCTSLRQDWAAPGIPSDLLNPDLQQA
                     PVAEHGFEDWSDRLDAGQVVLEHFTDADRGHPLAPDARSLLVIPVFAGSERWGALRLT
                     SLTPRDWPAAEVDAARSVAVNIGSALQRRRIFADLRRLSMAIEQAIEAVVLIDINGRI
                     QYVNRAFSKVTGYAPLEALGQDFFGLNLQEGSRDLPPDLWDTLLTGRVWDGRVKGRKK
                     EGRPFDEEMSISPARDDDGRIVNFVVVLRDVTEQLRLDQQLRQSQKMEAIGQLAGGVA
                     HDFNNLLQVIQGYSQLARRELAADARLREHLDQVLKASERAAVLVSQLLTFSRRQVRQ
                     PRRVDLNVIVDDVVRMLRRVIGEHIHLEPHTAAVPCLVQADTGQLEQVLMNLCVNARD
                     AMPHGGRLRVHCGQREFTPDDCLGEPWARPGQFAWLGVEDNGVGIPPDALEHIFEPFF
                     TTKDKGRGTGLGLATVYGIVKQHDGLISVQSEPGRGTFFRVYLPLAQGDGLSEGAAEH
                     EELQAGHGLILLAEDEELVRNLAVRILESAGYRVLVAHDGQQALDLLEQHADQIDLAL
                     LDMVMPRVSGRTVYEHIRRNRPSIPVLFTSGYSTDVLDSTMIPDENFHLIEKPYQPQL
                     LLRRISELIRPGRR"
     gene            10339..11139
                     /locus_tag="PLS90_04445"
     CDS             10339..11139
                     /locus_tag="PLS90_04445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPK01687.1"
                     /translation="MHRTCLALLGLLTLSGAARAAIHAETVEYQDGETKLVGYLAYDD
                     AMEGPRPGVIVVHEWWGLGEHVRTSAQNLARMGYAALAIDMYGEGRLTDDPREARELS
                     SQFKNDPALARARFEAGMKVLREHAACDPERIAAIGYCFGGTVVLSMARQGLDLDGVV
                     SFHGGLSGIAPAAGDAIKAAILVLHGGDDPAVPPADLTAYIEGLKAAGADWEVVIYGG
                     AVHSFTNPQADGSNPGSKYHPEVAGRAWGAMRDFLARVFEKPQTAEAP"
     gene            11185..12087
                     /locus_tag="PLS90_04450"
     CDS             11185..12087
                     /locus_tag="PLS90_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPK01688.1"
                     /translation="MARYPILSMTGYGRAQSATPFGQFTVEVRTVNNRFQDVSISAPR
                     EMPQLESPLRNLLRSRIARGKIDCRVRHAPLADQALPQVRINVEAARVYIHELEKLSR
                     LGAAPEIPLQMLTQLPGVMEIQTAEIDEQALWEAVAPVALRALDALDEERGREGEALA
                     AQLGQLVDELERLVEEVEAKRSAVVERFRERLAARVAELQDSLRAQLEPGRLEMEVAL
                     YADRGDVSEETVRLRAHLARARELLQPDGREPVGKNLDFLNQEILREINTICSKARDT
                     ELTATGLGMKSIAEQMREQVQNIQ"
     gene            complement(12139..13395)
                     /gene="fabF"
                     /locus_tag="PLS90_04455"
     CDS             complement(12139..13395)
                     /gene="fabF"
                     /locus_tag="PLS90_04455"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPK01689.1"
                     /translation="MAQVRRVVVTGMGAVTPIGNNVADFWRNLKAGHSGIRRITRFDP
                     KDVACQIAAEIKDLNYEDHFDKKEIRKNEDFTKNAVIAAREAAAQSGIRDAGLAPEEI
                     GCVLGVGIGGIGFIQEQVLAMKENGARRVSPMLIPKIIANIAPGQMTIDLELRGPSMS
                     VVTACAAGTHAIGEAANMIQRGDATAMLAGGSEAAICEIAIAGFSNMQALTTKFNDRP
                     EEGSRPFDSQRSGFVMGEGAAILVLEEYEHARARGAEILAEVLGYGLSSDAFHITAPA
                     PGGEGGARAIALALKRAGLPPTEIQYINAHGTSTPLNDKNETEAVKAVFGEHAYKLKM
                     SSNKSMIGHLLGAAGAVEAVATIMSIREQIAPPTINFTQPDPDCDLDYVPNQAQPCPI
                     EHAISNSLGFGGHNCTIVLGRVNGSR"
     gene            complement(13567..13812)
                     /locus_tag="PLS90_04460"
     CDS             complement(13567..13812)
                     /locus_tag="PLS90_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012462771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPK01690.1"
                     /translation="MASAEERVKKIIVEELGVNEDQVKPEAKFTDDLGADSLDQVELI
                     MRFEEEFGVEIPDEDTEKIITVGDAIRYMQEKTQEQA"
     gene            14665..16149
                     /locus_tag="PLS90_04465"
     CDS             14665..16149
                     /locus_tag="PLS90_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01691.1"
                     /translation="MLFILAAFAAFSLYELRDQDMDSDELTTSRVIRMPTQDLIRERL
                     RKGHPPLYFLLAKGWVTLSGHRASTLVGLSVMIAATSIVLFHLLCRELGLGRWAWAGT
                     LLWGLHPTVIYFGRYARPYAGVDLFIVAGLWLTLLALRRKDAPSYLALGAVGALAGAW
                     NHLLLLFWAGILLALALIGPLRRDTRRGLWLALAVPVAVHGAFLILIASMAGSKPIAW
                     IEKPRLLATANLFLELLGGRPLWIKMPGWTWTHPALLAVGTALGCWAVFNQSSRSGAR
                     QAPADGGPPLVHRWQVVAMTTLVPLAIMVWVTFTFQPLLVRRYMNILLPGAVLFGVWL
                     LAFMPYRRLAQGLLVAALTTQLTIARQEVRTRDERGMQKLVRALAQHYVEGEDVVLAF
                     NQSSDEAIELFSGHTIPSTDIYREMPHELAWPIFRSLVEGKKRLWIYCYRHQGSLYYS
                     PHWEERAGKLFYAKEERLCQLYGYNLPAEQPAYGPDSGGGAEAR"
     gene            complement(16159..16599)
                     /locus_tag="PLS90_04470"
     CDS             complement(16159..16599)
                     /locus_tag="PLS90_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPK01692.1"
                     /translation="MPPLPPPHVLQHAIETRFVCHRCGHCCKGDGLVQFGPLEADRMA
                     DHLGLSRRQFLKQFALRVEDDQWILRDRFVQDASTAGRREQWCIFLERDADGRYGCRV
                     NPAKPDQCGSFPAQWRNPDSLQTCVGLRRLLADLKAEPAAPKAP"
     gene            complement(16739..17062)
                     /locus_tag="PLS90_04475"
     CDS             complement(16739..17062)
                     /locus_tag="PLS90_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HPK01693.1"
                     /translation="MADPIIVTGCRIYLNYGADEHLKAFASIILNDAFAVCDLKVISG
                     SKGLFVAMPSRRRKNGVFLDVAHPVTKELRMHIEQTVLTEYQRALEDHTRRRRKQPVQ
                     HGSPV"
     gene            complement(17140..18057)
                     /gene="ispE"
                     /locus_tag="PLS90_04480"
     CDS             complement(17140..18057)
                     /gene="ispE"
                     /locus_tag="PLS90_04480"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPK01694.1"
                     /translation="MSAPPATALRLFAPAKFNPYLAVRRRRPDGFHDLDTIFQALNWG
                     DELRCRPLDQDVCRIACDHPGVPLGPGNLIARAWERLRAAFPGRVGGLAVDLIKRIPP
                     GAGLGGGSADAAATLVALNRLYHLRLPAHALEELAADLGSDCPFFIRGGTQVGSGRGE
                     QLRPIRNRLPPRWIVGVWPGFPSSTAAAYRRITPADWHDGHGLAAAIDAIERGDWIAL
                     QKCMYNTFSTLVSDTDLRYNTLREEISAHGLTHPLLAGSGSSIVAFAASHAAASAAAR
                     RLAGGSRVVFISRFRRRGVGLLAPRRSGL"
     gene            complement(18054..19196)
                     /locus_tag="PLS90_04485"
     CDS             complement(18054..19196)
                     /locus_tag="PLS90_04485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01695.1"
                     /translation="MRIAIFLDTDHLSGPARLARDFAVDARRLGHQIVMVGLVRGAPA
                     HDNAFTRAMADADLPTEILRERFRFDPAIVPQFLRLLDRFQPDLYQSHGYKGSFLGCR
                     ARRGALRWQAVFHGFTWENWRVRLYHALDARWLRRADEVVVVSRPFGRELARRGVQTD
                     RIRWVPNAIAPLPSAPRDARLRRDLGAAAPADFLVGCIGRLSPEKGPDIFIRALALAA
                     AGRPHLRAVLVGDGPLRDACRRLIAESNLADRVRLAGFTDNPAAIYPQLDLLVIPSRS
                     EGLPTVLLEAMQTGVPVVSTRVGGVPDLVRDGETALLAPLNDPPALAAAIGRAADDPA
                     LREHLARQAGRLALERLSVAARARTLLDHAAALVAGRPLPPEEPLP"
     gene            complement(19430..21106)
                     /locus_tag="PLS90_04490"
     CDS             complement(19430..21106)
                     /locus_tag="PLS90_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01696.1"
                     /translation="MSSPFSSSPHLLVSDGYLHAAFRHGEEPLLVLDGRSLTVVDANL
                     AAGQLLGISRGDLIGCPILQLINDDSQRAVFSRMAGMLSGTAEHVPVLEGVRVLDADG
                     AMHACRLRLAGCDPAGSRPLLLAWLKPETESGPADEALARAARAACMESLLEIISEGY
                     LAIDGTGHISYANSRALRTLHRSAGEVLGLTLQRLFPLLADTSLKEVLAARDEDTNPD
                     RLTFAYIAEGETRRFQYSVHPQGDGLGFFFRDISHQTERDIELAKLKATMAASQELLR
                     RKNDELNASLEQLEQMNEALARADQTKSEFLANTSHELRTPLNSIIGFLQLILEGLCE
                     SPEEEREYIANALASAQHLLQLINDVLDIARIEAGRMNFLIDDFPVEKLLREVESLAS
                     VQATRAGLHLSFEVRAADPEELLAHADFHRTKQILINLVGNAIKFTEEGAVRVWAEVD
                     PEQAGSLLIVVEDTGIGIAPDQQQKIFEKFTQADGSSTRRYEGTGLGLAITRNLVELM
                     GGTIGLHSPGSGKGTRVWFTLPLGGKKPIMELSDELDNDDDQEDCLPTTF"
     gene            complement(21326..21763)
                     /locus_tag="PLS90_04495"
     CDS             complement(21326..21763)
                     /locus_tag="PLS90_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01697.1"
                     /translation="MAVVVPLALCLLLGCRSPRAGDRESPPGDYETARPAAEHPTDTW
                     DAWLEQTDDGSPRLIVSGRVQVPTPCHEANLAAAQTPAGRLSALPLELIVSAPAGICA
                     QVVSSVEARYERQEGMEDVKTVRIHYPGGSVWEVPVKPRQPAL"
     gene            21898..22893
                     /locus_tag="PLS90_04500"
     CDS             21898..22893
                     /locus_tag="PLS90_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK01698.1"
                     /translation="MRNVESRIASRGGMLFDAAELAALTTGGDGLFRAGENGVRAILE
                     PKDGKAEFIVFEDGRVLAHIRSRMGYPAFYPLHPARIERPVEAVLMDLDGTSVRSEHF
                     WIWIIEQTTARLLGNPKFELEEADLPHVSGHSVSEHLRHCIAKYAPEKTVEEARRHYF
                     DITRHEMNEIMEGRGKDDAFTPTEGLKEFLLELKGRKIRIGLVTSGLYEKAWPEILAV
                     FRQLGLGDPREFYDAIITAGHAIRKGEAGTLGELEPKPHPWLYAEAARVGLGIEFGRR
                     HHVVGMEDSGAGVISIRLAGFTCLGVAGGNIASSGTGSFCSRVCRGLEEALAEIV"
     gene            complement(23003..23800)
                     /locus_tag="PLS90_04505"
     CDS             complement(23003..23800)
                     /locus_tag="PLS90_04505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK01699.1"
                     /translation="MPLDPTRHGPAALVTGASSGIGREYARALAARRFDLVITARRRD
                     RLEELRNELTQRHRVRVELIEQELAAPDAGQRIERRVAELGWGIGLLVNNAGFGLYGR
                     LDQTDPARQVAMVDANCRAVLDLTLRFLPPMVRRRRGGLIIVSSVLGFIPTPFYSVYS
                     ATKAFDLLLAEALHAEYRDLGIDVLAVCPGMTRTEFNAGAGIKSVGRFNREPEQVVES
                     SLMLLGRRAFMVDGWWNRLLLAALRCVPRRLMLAGSHRIMTRRNPEA"
BASE COUNT         4004 a         6663 c         9060 g         4160 t
ORIGIN      
        1 gcgctgtatg aggtgatggt gatgagcgac cggctgcgcg atgcggtgat cgacggggtg
       61 tcgacgaacc agctcaagcg gctggcgatg gccgagggga tgcagacgct gcggatggcg
      121 ggaattaaca aggtactgga gggtcggacg acgatcagcg aggtgctgtc ggccacgatg
      181 gcggacaccg ttcaagagat cgccgtggcg accgataagg aagaccaggt ggcctagagc
      241 gcccagccgc agcctgccga cgcgtaccca ttgaaccgga gaaggatcga tgcccgtctt
      301 ccagtactcc gcccgcgaca atcgcggcca gatcatccgg ggccagaccg aagcccagaa
      361 tcaggcggcg gtgatccggc tgctgcgcga gcaggggctg atcccgacga cggtggaggc
      421 gggggcgacg cgccgtgcgg cccgccgcgc gatgggcaag ggggggaaga tcaagctgga
      481 ggacctggtg atcctgacgc ggcagttcgc gacgatgatc cgggccgggc ttccgctgat
      541 cgaagtgctc aacatcctgt cggaacagac cgagaagcgc gccctgaagg cggtaatgaa
      601 acaggtggag cgggacgttg agaccggctc gagcctgaac gaggcgatgt cgcgccatcc
      661 gaaggtgttc tcgacgtttt atctgagcat gatcgcggcg ggcgaagccg ccggcatgct
      721 cgacagcatt ctggatcaga tcgcgctgta tctggagaag gtcgtgtcga tccagcgcaa
      781 ggtcaagtcg gcggtgatgt atccgtcggt cgtgtcggtg gtggcgatcg gcatcaccgt
      841 gttcctgctg atcaaggtgg tgccggtgtt cggcgacatt ttctcggaca tggggggcaa
      901 gttgccggcg ccgacgcgga ttacgatggg gatttcgaat ttcctgcagg acaacttcct
      961 gctgtcggta ttcatcctga tcggtctggc gctgctggtc tggcaggggg gccggacgcc
     1021 gaaagggcgg cgcatcctgg accagctgaa attgaagatg ccgatcttcg ggccgttgtt
     1081 tttgaaggtg gccgtcgcac ggttcacgcg gacgctgggg acgctgatac attcgggggt
     1141 gaacattctg gcggcgctgg acatcgtggc gcggacggcg ggcaacgtgg tcatcgaaga
     1201 ggcggtggtc aagacgcgcg cctcgatcca gagcggcgaa tcgctggcgg cgccgctgcg
     1261 cgacagccag gtgttcccgc cgatggtggt gcggatgatc gacgtgggcg agcggaccgg
     1321 cgcgctggaa tcgatgctca cgaagatcgc ggatttttat gaagaccagg tcgatgcggc
     1381 ggtgtcggga ctgacgtcga tgatcgagcc gctgctgatc gtcttcctgg gcgtggtcgt
     1441 cgggttcatc gtgatttcga tgttcatgcc gatgttcaag atgattgaac tggtgggctg
     1501 agtcgactcc acgaccgacc ctcctccgat ccccggccgg gcctccgctc ggccggggct
     1561 ttttttgttt tggggggagg cggggaaggt ggtcggcggg gcagtccggc atccgaccaa
     1621 cgcgcgtccg gggatcatcc tcgtgattgg ctcctgcgcc cggtccagcg cgcgtcggtc
     1681 cggacgtcgc ttcccatcga gtacggcggg gaaccggttg tgggcgtcat ccgcacggtt
     1741 cgcattttca ccgagtattg aaagcggcag caggtgaaaa gattgctttg aagaagcaac
     1801 cgcacttgct gtaatgtctg gtgagtggaa tttcattccg atggcgccgg gccgaacacc
     1861 gacagacgct ggagcgaacg gtatgaagtg ttacactgaa agttttccag ggaatcgaac
     1921 attcgctgaa ttcttcgccg gtatcgggct gatgcggctt ggcttggagc aggctggatg
     1981 ggaatgccat ttcgctaatg atatctgtcc tgataagttc gcaatgtata aggataactt
     2041 tccagaatct gatgatcatt ttagattggg agatattcat aatctgaagc ctcaggatgt
     2101 ccctacggtc gccttggcta ccgcctcctt cccctgtaat gatctttcat tggcagggat
     2161 gagaaaggga ttggctggga gccaatcatc cgctttctgg gggttctgcg aaatcttgag
     2221 aggactgggc tcccggaagc ctccgatcgt tttgctggag aatgttggtg gtttccttac
     2281 atccagaggg ggcgcggaca tccgagaagc gctcttggca cttaatggtc tcggctacgt
     2341 cgtagacgcg ttcatcatcg acgcagagcg ctttgtacct caaagtcgag tcagactatt
     2401 tattatcggt caccaattca atgatggtgt gcttgacccc caacgctttt acgagtcgcc
     2461 ggttcgcccg cgccttctgg cggacttcat tttctcgcat ccagagattg agtggagcat
     2521 cagggacttg cccgatcttc caaagcgtca gattacgctc cgcgacattc tagaagatgt
     2581 tgatccaaat tcgaagatct ggtggagcga ggaaagaacc aattacctgg tgtcacaaat
     2641 gagcgaaagg cattctgccg tcctgaaggt actgatgggg aagcgagctt ggagttatgg
     2701 caccgtattt cgccgtattc ggaacggaag gtccatggcc gagcttcgag cagacggcat
     2761 tgccggttgc ctgagaactc caagaggggg aagtggccgg caaattctaa ttaaggcggg
     2821 ctacaataaa gttcaagctc gcttgctcac cccacgcgaa tgcgcacggt tgatgggtgc
     2881 taatgaatat aagatcagtt gtccattgaa ccaggcatta ttcggatttg gcgacgccgt
     2941 atgcgtgcct gttgtaaagt ggattgctga caattatctt aatcctctcg cggatcgtct
     3001 ctcactgccg ttcgcccaac tcgcttagcc aggtgattcc gtcaacggtg gtcaaagata
     3061 aactcgagac ccatctggag catttcacgc gcgagaatca aatatcgggc aaggggccct
     3121 tgtccctcgt actcatcctc actagagacg tatcgcgtcg cgagtttccg ctcaaggcca
     3181 aggatttcgt tacaggcaag aaagggcagg tcaaggggtt gagcggatcc aagatccaaa
     3241 caattctccg tgatcatggc atcacgcaaa gactcgcgga ggagggaggc cggaccagtc
     3301 gaggcagcat cgagaagatg gagctttacg tcgacttcct caacaagctg ttcgaaaagg
     3361 acctgctgga ctttgagcgg attgagatgt ggtggattgc gagggtcaag gagcatttcg
     3421 cgcgtctgcc atttcgtgtg aggctcgact cctcaaagtc cttgcgattc atctttggcg
     3481 aactgttagc ggcaacggtt gcaaggcaaa gggcgtgtcc aggaatgatg atcaccggcg
     3541 ccgtgatgca acatctggtc ggagcaaaat tgcagctgtt gtttcccgat ttgaagattg
     3601 aacaccatgg cttttccgtg gcagattcct ccaccatgcg agttggagat tttaaggttg
     3661 aggatgcaat catccatgta acttcggccc ccggtgaacc gcttttgagg aaatgcgcag
     3721 acaacctaag cgcaaaccta agaccactta tcatcacgac ggagaccgga gttggcgggg
     3781 cacgggctct tgcctcaaat ctgggaattg aggatcgact ggacgtcttg gagattactc
     3841 agtttctcac ggcgaatgtg tatgagtgga gcagattcag ccagaaggag catcccgctt
     3901 cggtcatcag tctgattaac acatacaata gaatcattga cgaatgtgaa agcaatccaa
     3961 gtttgcgcat ccaagtgact taggcgccat ggacagtttc accccccagg aacgaagcgc
     4021 gatcatgcgc ctggtcagat caaaggacac caagccggag cgtcaagtca ggtcattgct
     4081 tcatcggctt ggattgcgct ttcgattaca tcgcggggat ttgccaggcg ctcctgatat
     4141 tgtgttgatt cggtataaga cggtcatttt tattcacggc tgtttttggc acaggcaccg
     4201 cggatgcaac cgggccagca ccccgagttc cagacgagaa tattggcttc cgaaatttga
     4261 acgcacggtt gcaagagacg ctcataaccg acggaggttg aggcagctag gttggagagt
     4321 agtgacgatt tgggagtgtg aactcaggca cctggaacgt ctcgagcgac gactcaggcg
     4381 gcttgtccaa gtcatccagt ccgactgaaa aagcgcatct tccaatattc cattttttcg
     4441 agcgcgattc ccttggctac tttctggcat ccctgccggg atgcggggcg ccgttggggg
     4501 caaggatgaa ttgcgcatgg atttttttgt tcacggtgag cagacaaaac gggggttttc
     4561 gagagccgag gacgagggac gagagccggg agccgaattt gggaatggcc gatgggggga
     4621 gatcaggtgt tgatcatgaa cccgcggttc acaacgggag acaaaatggc gggtttcgag
     4681 agccgaggac gagggacgga agtggggggt tagtcgggga gcgagggggt gggggcaggg
     4741 aggaagtctt cggcggcggg ggcgacgggg gtgacgtcga gcggctggag ttgtttcagc
     4801 tgcttggatg agtggacgcc gagttcgttg aagcggcggg cctggacgag gacgcgcgat
     4861 tccatcgagc cgaccgcccg gttgtaatgc cggacggtct gctccaggga cgagccgacc
     4921 ttgcccagat ggtcgccgag cgtgcagagc cgctcgtaga gttcgcggcc gaggtcgctg
     4981 atgcgctggg cgttttcggc gagttgctcc tcgcgccagc cctgggcgat cgtcttgagc
     5041 agcgaaatga gcgtgctcgg cgtggcgagg atgacgttgc gttcgaagcc gcgctcgatg
     5101 agatcggggc gctgatcgac ggcggcggcg aggaagcttt cgcccgggat gaaaagcacg
     5161 acgaaatcgg gggcggggcg cagctgggcc tgataggcct tctgcgcgag gcgttccatg
     5221 tgctcgtgaa gctggctggc gtgacgctgg agggcggcgc ggcgctggtc ggggtcggcg
     5281 gcctcgaggg cctccagata ggccatcagg ggggtcttgg cgtcgatgac gatgcagcgg
     5341 ccgccgggaa gattaacgat catgtcgggc cgcaggcggc cgtcggcggc gtcgatggcg
     5401 acctgttcgc ggaagtcgca gcgatcaacc atgccggcca gttcgacgac gcggcgcagg
     5461 gtgatctcgc cccagcggcc gcggacctgc ggggcgcgca gggcctggac gagcttgccg
     5521 gtttcctgtt cgagctggcg ctgggtctgg ccgaggtggg cgacctgcgc gagcagctcg
     5581 ccgtgggcct tcgcgcgcag cgtctccatt tcctgaatct gcttttcgta gcggctgagc
     5641 tgttcgcgca agggggcgac cagttcgccg atcgcctgct ggcggcgctc caggtccccg
     5701 cgggccgcct cctggtgctg gcgcaggttc tgcgtggcga gttggaggaa ggcttcgttg
     5761 ttcttgctga gggcctcgga ggcaagggag cggaagctgt cggtgaggcg ggcgcgggcc
     5821 tcgtcgagga gggcctgttt ttcctgggcg gccttgcgtt cgtcgtcgag cgcagtctgg
     5881 agggcggcga ggcgttcggc gcgggcgcgc agctcggcgc cgagcgagcg gatctcgtct
     5941 tccagccggg ggacgcgttc ggcctgggct tcggcggcgt ggaggcgtcc ggtcaggtcg
     6001 gtgacgcgct cgcgcagctc ggcggccagg cgggattcct ggtcggtccg ctgggtcagt
     6061 tcgtcgaagc ggcggcgggc ttcgtcgagg tcgcggccga gcagcgccgc ggtcgcgtgc
     6121 gcccgggcca gccgcgagcg gaggaagagc gccgccgccg ccgcgccgac gagcagtccc
     6181 gtgacaagcg ccaagacgat ttccatgatt tccccctttc gcgccgatcg agcggttgct
     6241 agcgtctgcc cggccggatg agttcgctga tccggcgcag gaggagctgc ggctgatacg
     6301 gcttctcgat cagatgaaaa ttctcgtcgg gaatcatcgt gctgtcgagc acgtcggtgc
     6361 tgtagccgct ggtgaaaagg acggggatgg acgggcggtt gcgccggatg tgttcgtaga
     6421 cggtgcgtcc gctgacgcgc ggcatgacca tgtcgagcag cgccaggtcg atctggtcgg
     6481 catgctgttc gagcaggtcg agggcctgct ggccgtcgtg ggccacgagc acgcggtagc
     6541 cggcgctttc gaggatgcgg acggcgaggt tgcggacgag ttcctcgtcc tcggcgagga
     6601 ggatcaggcc gtggccggcc tggagttcct cgtgttcggc ggcgccctcg ctcaggccgt
     6661 cgccctgcgc cagcgggagg tagacccgga agaacgtgcc gcgtcccggt tcgctctgga
     6721 ccgagatcag gccgtcgtgc tgcttgacga tgccgtagac ggtggccagg ccgaggccgg
     6781 ttccgcgccc cttgtccttg gtggtgaaga agggctcgaa gatgtgttcg agcgcgtcgg
     6841 ggggaatgcc gacgccgtta tcctcgacgc ccagccaggc gaattgaccg ggacgggccc
     6901 agggctcgcc gaggcagtca tcgggggtga attcgcgctg gccgcagtgg acgcgcagcc
     6961 ggccgccgtg gggcatggcg tcgcgcgcgt tgacgcacag gttcatcagg acctgttcga
     7021 gctggccggt gtcggcctgg accaggcagg ggacggcggc ggtgtgcggc tcgaggtgga
     7081 tatgctcgcc gatgacgcgg cgcagcatgc ggacgacgtc gtcgacgatg acgttcaagt
     7141 cgacgcgacg gggctggcgg acctgacggc ggctgaaggt gaggagctgg gagacgagaa
     7201 cggcagcgcg ctcggaggcc ttgaggacct gatcgaggtg ttcgcgcagg cgggcgtcgg
     7261 cggccagctc gcggcgggcc aactgggaat agccctggat cacctggagc aggttgttaa
     7321 agtcgtgggc gacgccgccg gcgagctggc cgatggcctc cattttctgg gactggcgca
     7381 gttgctgatc gagccggagt tgctcggtca cgtcgcgcag gacgacgacg aaattgacga
     7441 tgcggccgtc gtcgtcgcgg gcgggggaga tcgacatctc ctcgtcgaag gggcggcctt
     7501 ccttcttgcg ccccttgacg cggccgtccc agacgcggcc ggtgagcagc gtatcccaca
     7561 ggtcgggggg gaggtcgcgg ctgccctcct ggagattgag gccgaagaaa tcctggccga
     7621 gcgcttccag cggggcgtag ccggtgacct tgctgaaggc gcggttgacg tattggatgc
     7681 ggccgttgat gtcgatgagg acgacggcct cgatggcctg ctcgatggcc atgctgaggc
     7741 ggcgcaggtc ggcgaagatg cgacggcgct gcagggcgga gccgatgttg acggcgaccg
     7801 agcgggcggc gtcgacctcg gcggcgggcc agtcgcgcgg ggtcaggctg gtgaggcgga
     7861 gcgcgcccca gcgctccgag ccggcgaaga cggggatcac gagaagtgaa cgcgcgtcgg
     7921 gggcgagcgg atgaccgcgg tcggcgtcgg tgaagtgttc gaggacgacc tggccggcat
     7981 cgagccggtc gctccagtct tcgaagccat gctcggccac cggggcctgc tgcagatcgg
     8041 ggttgagcaa gtcggatgga atgccggggg cggcccaatc ctgccgcagg ctggtgcagc
     8101 ggctgccgtc ggcgttgcgg gtggtttcga agagctgcac gcggtcggcc tcaagggttt
     8161 cgcccaggcg cgagaggatc gaggggaggg cctcgtggag ctgctgcgcg cggagcaggc
     8221 gctcggagat ataggccatc gcctcgagga tctgctcgcg gcggagcaga ttctcctcgg
     8281 cctgcttgcg cgagctgatg tcgcggatga cgttgaccac gccggagatc cagccgcctt
     8341 cctcggtgat gcgcgtgacg gtgtggtcgg tatggatgac gcggccgtcg cggcagcgca
     8401 tgcggcactc caggtgcagg ctttcgcttt gcccggggct gccttcgaga aagtcgcgca
     8461 ggcggcgata gttatcgggc tcgaggtgga gcgcttcgac ggactggtcg atcagttcca
     8521 ggcgcggata gccgaacagg cgctcgacgg cgggattgca ggtcaggatc cggccgctgg
     8581 cggcgtcgat caccagcacc gcctcgtgca tgctggtcag cgttttttcc atcaggaagc
     8641 gcgtgtcgag gagttcctgt tcctgggcct tgcgctgcga gatgtcctcc atggcgcaga
     8701 tcatgtagcg ctggccgttg atgcggtcct cgacgacgga gacggtgagg ttggcccaga
     8761 agaactggcc gttcttgcgg cggtagcgtc gttcgccggc gaaggtagtg acttcgcccg
     8821 acgtgatgca ttcggcctcg gccaggccgc gctggagttc gccctcgggg atcaggtcgg
     8881 cgaagctgag gcgtaacagt tcgtcgcggt cgtagccgac gatctcgcag aagcgctggt
     8941 tgacgcggag gaagttgccc tgcatgtcgg cgtgcagcag gccgacggcg gcctgctcaa
     9001 acgtcgagcg gaagcgttcc tcgctctggc gcagggcatc ctcgaagcgg cgccgatgga
     9061 ggaactcgcc gatgcgctcg gccagggcgc gggccaattg atcttcctcg gggagaaaaa
     9121 tccgccgcga ggaaccgggg gggcgttcgg gataaccgac gagcaactcg ccgatggctt
     9181 cgccctcgag ggcgatcggg acgcactggg tgaattgcgg cgggcgccag ccgtcgctga
     9241 gaaacgacgc ctggtcgacg cggatgcgca cgacgcacat ttcgggaaac tgccagccgc
     9301 ggcggatcag ctcgggcaac tgggcgaggc attcggcgat cgttccgccc gcgtcgatga
     9361 gcaggcgcga gacctccagc aggcaacggc tttccttgac gcgttccttg agatcgccgc
     9421 gcaggcgttc cagctcgtgg tcgcgctcaa cttgcgccgt gacgtcgagg agcgtgccga
     9481 cggtgagggg gtggccgcgg tcggcgccgt cgccggtggc ggggatgcgg cggcaacgcg
     9541 cctcgagcca gcgcgcggca tcgcccggcc gtgggatgcg gaagcggaag ccgtcggagt
     9601 cgccctcggc gggaccttcc tggataaagc ggctcagcgc cggccggtcc tcgggatgga
     9661 ccggggcgag gagctccgcc aggtcgtcgc cggcgcggga ggaacaaccg aggagttcaa
     9721 cgcagcgcgg ggaagcgcgg aaccggccgg tccgtggttc ccactgccag acaccgaggc
     9781 cggcggctgc ggccgcctgg tgataggcct cctggcggaa gtcggcggcg gcgcgagcgg
     9841 gcgggaggcc gacgtcttcc agcgaatggg agcggtttgg cgaggttgga gtcatgcggg
     9901 aaactgcctg ggacggcatg aaggcgaccg agtcgatata gcggctgggg cgcacccggc
     9961 gcagatcgcg aggcaggcgg gctcgggccg gtgaccggca aaggccggtt catcactcaa
    10021 acagtatcca ccgcgtgggt tgcatttcaa ctgtataggc gagtttgatt ggcaggggtg
    10081 atctggcatc cgtgccggga tgcggggatg ggcggcctgc tgaccggtgg tgtcgttgcg
    10141 ctcaaccacc ggctactgtc tggcatccct gacgggatgc ggggtgaggg actgagggac
    10201 gaccggcgag ggcgccggtc cccacgacgt tcgttgatgg gcggtgggat tccacgtttg
    10261 cgcggggcgg gggatccgcg atatcttgtt gatatcgctt cataatcccc ttggatctgt
    10321 ccggaaggga gacgaatcat gcatcgcacg tgccttgccc tgctgggcct gctgacgctg
    10381 agcggagcgg ctcgcgccgc gatccatgcc gagacggtcg agtatcagga cggcgagacg
    10441 aagctggtgg gctatctggc ctatgacgac gcgatggagg ggccgcggcc cggggtgatc
    10501 gtggtccacg aatggtgggg cctgggcgaa catgtgcgga cgagcgcgca gaatctggcg
    10561 cggatgggtt acgccgcgct ggcgatcgac atgtatggcg aggggcggct gacggacgat
    10621 ccgcgggagg cgcgcgagct gtcgagccag ttcaagaacg acccggcgct ggcgcgcgca
    10681 cggttcgaag cgggaatgaa ggtgctgcgc gagcacgccg cgtgcgaccc ggagcggatc
    10741 gcggcgatcg gctactgttt cggcgggacg gtcgtgctga gcatggcgcg ccaggggctc
    10801 gatctggacg gcgtcgtcag ttttcacggc gggctgagcg ggatcgcgcc ggcggcggga
    10861 gacgcgatca aggcggcgat cctggtgctg cacgggggtg atgatccggc ggtgccgccg
    10921 gccgacctga cggcatatat cgaggggctc aaggcggccg gggcggattg ggaagtggta
    10981 atctacggcg gggcggtgca cagcttcacg aatccgcagg ccgacgggag caatccggga
    11041 tcgaagtatc atccggaagt ggcggggcgc gcgtgggggg cgatgcggga ttttctggcg
    11101 cgtgtctttg aaaagcccca gactgcggaa gcgccgtagg ccgcccgccc gcgcggattc
    11161 tgttctggag tcagttcgac cgccatggcc cgttatccga tcctgagcat gaccggctac
    11221 gggcgcgcgc agtccgccac gcccttcggg cagttcacgg tggaagtgcg cacggtgaac
    11281 aaccggtttc aggatgtgag catttcggcg ccgcgcgaga tgccgcaact ggaatcgccg
    11341 ctgcgcaacc tgctgcggtc gcggatcgcc cgggggaaga tcgattgccg ggtgcgccac
    11401 gcaccgctgg ccgaccaggc gctgccgcag gtgcggatca acgtggaggc ggcgcgggtc
    11461 tacatccatg agctggagaa actgagccgg ttgggggccg cgccggagat cccgctgcag
    11521 atgctgacgc agttgccggg ggtaatggag atccagacgg ccgagatcga cgaacaggcg
    11581 ctgtgggagg cggtcgcgcc ggtggcgctg cgggcgctgg atgcgctgga cgaggagcgt
    11641 ggaagagaag gcgaggcgct cgccgcgcag ttgggccagc ttgtcgacga gctggagcgg
    11701 ctggtggagg aggtggaggc aaagcggtcg gcggtggtgg agcggttccg ggaacggctg
    11761 gcggcgcgcg tcgccgagct gcaggactcg ctgcgcgcgc aactggagcc ggggcgcctg
    11821 gagatggaag tggcgctgta tgccgaccgg ggcgacgtga gcgaggagac ggtgcgattg
    11881 cgggcgcatc tggcgcgcgc gcgcgagctg ctccagccgg acggccgcga gccggtgggg
    11941 aagaacctgg atttcctgaa tcaggaaatc ctgcgggaga tcaatacgat ctgttcgaag
    12001 gcgcgcgata cggaattgac cgcgaccggg ctggggatga aatcgatcgc cgagcagatg
    12061 cgcgagcagg tgcagaatat ccagtagcgg cgggggcgcc ggtcctgccc aggccggtgt
    12121 ttccgaagtg gccgcgggtc agcggctgcc gttgacgcgg cccagcacga tggtgcagtt
    12181 gtggccgccg aagccgagcg agttgctgat cgcgtgttcg atcgggcagg gctgcgcctg
    12241 gttggggacg tagtcgaggt cgcagtcggg gtcgggctgg gtgaaattga tcgtgggcgg
    12301 ggcgatctgt tcgcggatcg acatgatggt ggcgacggct tcgacggcgc cggccgcgcc
    12361 gagcaggtgg ccgatcatcg acttgttgga actcatcttg agtttgtagg catgctcgcc
    12421 gaagacggcc ttgaccgctt cggtttcgtt cttgtcgttg agcggcgtgc tggtgccgtg
    12481 ggcgttgatg tactggatct cggtgggggg gaggccggcg cgcttgaggg ccagggcgat
    12541 ggcgcgggcg cccccctcgc cgccgggcgc cggggcggtg atgtggaagg cgtcggagga
    12601 gaggccgtag ccgaggactt cggcgaggat ctcggcgccg cgcgcgcggg cgtgttcgta
    12661 ttcctcaagg acgagaatcg ccgcgccctc gcccatgacg aagccgctgc gctgggaatc
    12721 gaacgggcgg ctgccctcct cgggacgatc gttgaatttg gtggtgagcg cctgcatgtt
    12781 gctgaagccg gcgatggcga tttcgcagat ggcggcctcg gagccaccgg cgagcatcgc
    12841 ggtggcgtcg ccgcgctgga tcatgttggc ggcctcgccg atggcgtggg tgccggcggc
    12901 gcaggcggtg acgacggaca tggaggggcc gcgcagttcc agatcgatcg tcatctggcc
    12961 gggggcgatg ttggcgatga tcttggggat cagcatcggg ctgacccggc gggcgccgtt
    13021 ctccttcatc gcgaggacct gttcctggat gaagccgatg ccgccgatgc cgacgccgag
    13081 gacgcagccg atctcctcgg gggcgaggcc ggcatcgcgg atgccgctct gggcggcggc
    13141 ctcgcgggcg gcgatgacgg cgttcttggt aaaatcctcg ttcttacgga tttccttctt
    13201 gtcgaaatgg tcctcgtaat tgaggtcctt gatttcggcg gcgatctggc aggcgacgtc
    13261 cttgggatcg aagcgggtga tgcggcggat gccgctgtgg ccggccttca ggttgcgcca
    13321 gaagtcggcc acattatttc caatcggcgt gacggcgccc atgccggtga caacgacgcg
    13381 acggacttgc gccatggcaa tgaaatcctt gaatagactg aaggtgcgag aggcggcgtc
    13441 ggccggggga gcggaagccg acgcgccgcg cgccggcttc gtcatgctgg ggggctccct
    13501 tcgccggccg gtccccgcaa ccgagggcag gggggcgcag ccggggcggc cgggaggcgc
    13561 cggggatcaa gcctgctcct gggttttctc ctgcatgtag cggatggcgt cgccgacggt
    13621 gatgattttt tcggtatcct cgtcggggat ttcgacgccg aattcctctt cgaaacgcat
    13681 gatcaactcg acctgatcga gggaatcggc ccccaggtcg tcggtgaact tggcttcggg
    13741 cttgacctga tcttcgttga cgcccagttc ctcaacgatg attttcttga cgcgttcttc
    13801 agctgacgcc atggaaatgc gcctccttgt ctgcggttcg tcggccgccg gttaccccgt
    13861 ccggctcgat cgggtggtaa ggatccaatc aggaaaggcg gcccggcgaa gggcatcctc
    13921 tcggctgtct cgattcggtc cgggcggggt ggatcgttca ggctcccgcg ggcccggcaa
    13981 actcaccagt ctatactggc tggattgggc attcgcaagc aaaaagagcc tcgtgtcccg
    14041 ttcatgatcc gccgcgatac aaccggctcg gatcgggaag cggatctatc gataacgcag
    14101 cgccggagct aaatcaatga gtttcagggg gatcgcggct ggcgaaaggg gatgcgggag
    14161 gggatcagga aagcgaggtg gatcatgaaa agcaggaggg gatcacgaac ggcacgaacg
    14221 gggaaaaatt tcgagagccg aggacgaggg acgagagccg agagccgaat tcccgggtgg
    14281 gcctttgggg cgcttaggag ttgcacctgc acctgcggtt cacaacgagg ggacgaaatt
    14341 gggcaggcct tcgggagcga tcacgaggac gaggatgaga gtgaagagac gtggtattga
    14401 gtgggaacga ggcacggcgt ggttgtgggg ggttgacttt gggcgggggg tatcggcaag
    14461 gtggtaggct gactgagcgc gcgcccccca cgttgacttg cgggtaatcc gatgacgacg
    14521 agtgagttaa ccgaaccaga tccgctcgcg tccggcggcg cggcaacccg cggcgaacca
    14581 tcggcagatg ggccgtccgc cgcggcggaa gcgcgcgccg ccacgcgcgg cggcgtgtgg
    14641 gccggggtgc gccgatggct gccaatgctc ttcatcctgg cagcgtttgc cgctttttcg
    14701 ctctacgagc tgcgcgacca ggacatggat tcggacgaac tgacgacgtc gcgggtgatc
    14761 cggatgccca cccaagatct gatccgcgag cggctgcgca aggggcaccc gccgctgtat
    14821 tttctgctgg ccaagggctg ggtgacgctc tccggccacc gggcgagcac gctggtgggg
    14881 ctgtcggtga tgatcgccgc gaccagcatc gtgctgtttc atctgctgtg ccgggagctg
    14941 gggctggggc gctgggcgtg ggcggggacg ctgctgtggg gcctgcaccc gacggtgatt
    15001 tacttcgggc gctacgcgcg gccgtacgcc ggggtggacc tgttcatcgt cgccgggctg
    15061 tggttgacgc tgctggcgct gcggcgcaag gacgcgccgt cgtatctcgc gctgggggcg
    15121 gtgggggcgc tggccggcgc atggaatcac ctgttgctgc tgttctgggc gggcatcctg
    15181 ctggcgctgg cgctgatcgg cccgctgcgg cgcgacaccc ggcgggggct gtggctggcg
    15241 ctggccgtgc cggtggcggt gcacggggcg ttcctgatcc tgatcgccag catggcgggg
    15301 agcaaaccga tcgcgtggat cgagaagccg cggctgctgg cgacggcgaa ccttttcctg
    15361 gaattgctgg gcggtcggcc gctatggatc aaaatgcccg gctggacgtg gacccacccg
    15421 gcgctgctgg cggtcggcac ggcgctgggc tgctgggcgg tcttcaacca gtcgagccgg
    15481 tcgggggcgc gtcaggcgcc ggccgacggc ggcccgccgc tggtccatcg ctggcaggtc
    15541 gtggcgatga cgacgctggt gccgctggcg atcatggtgt gggtcacgtt cacgtttcag
    15601 ccgctgctgg tccgccggta tatgaacatt ctgctgcccg gggcggtgct gttcggcgtc
    15661 tggctgctgg cgttcatgcc gtatcggcgg ctggcgcagg ggctgctggt ggcggcgttg
    15721 acgacgcaac tgacgatcgc ccgccaggag gtcaggacgc gcgacgagcg cgggatgcag
    15781 aaactggttc gcgccctggc gcagcattac gtcgaggggg aggatgtcgt gctggcgttc
    15841 aaccagagca gcgacgaagc gatcgaactg ttctcgggcc atacgattcc gtcgaccgac
    15901 atctaccggg aaatgccgca tgaactggcg tggccgatct tccgatcgct ggtggagggg
    15961 aagaaacggt tgtggatcta ctgctaccgg caccagggaa gcctctacta ttcgccgcac
    16021 tgggaggagc gggccgggaa gctgttctat gcgaaagagg agcgtctctg tcagctctat
    16081 gggtacaatc tgcccgcgga gcagcccgct tacggtccgg attcgggcgg aggggcggaa
    16141 gcgagatgag cgcgtctgtt acggcgcctt gggcgcggcc ggttcagcct tgaggtcggc
    16201 caacaggcgg cggaggccga cgcaggtctg gagggaatcg gggttgcgcc actgggcggg
    16261 gaacgatccg cactggtcgg gcttggcggg gttgacgcgg cagccgtagc ggccgtcggc
    16321 gtcgcgctcc aggaagatgc accattgctc gcgccggccg gcggtgctgg cgtcctgaac
    16381 gaagcggtcg cggagaatcc actgatcgtc ctcgacgcgc agcgcgaact gtttaaggaa
    16441 ctggcggcgg gagaggccga ggtggtcggc catgcggtcg gcttcgaggg gaccgaactg
    16501 gaccaggccg tcacccttgc agcaatgacc gcagcgatgg cagacgaaac gggtttcgat
    16561 ggcatgctgg aggacgtggg gcgggggcag ggggggcatg ggtcgagtga atgaggggga
    16621 tggggcgcga aaagccgacc gtggcatcag cttttgcggc aggattcgat cagtttacct
    16681 gggatgagtg aggccggcaa gcggagcgcg ggagaaaacc cgaccgggga gcgttggctc
    16741 aaaccgggga gccgtgctgg acgggctgtt tccgtctgcg gcgggtgtgg tcttccaacg
    16801 cccgctggta ctcggtcagg acggtctgct cgatgtgcat ccgcagttcc ttggtgaccg
    16861 gatgggccac atcgaggaac accccgttct tgcgtctgcg actgggcatg gccacgaaca
    16921 ggcccttgct gccgctgatg accttcaggt cgcagacggc gaaggcgtcg ttcaggatga
    16981 tggaggcaaa cgccttgaga tgctcatccg cgccatagtt gagatagatc cgacacccgg
    17041 tgacgatgat gggatcagcc ataaaacaat ccctcggtgg aagaatccgg tctccggcgg
    17101 gaaccgatcg cgcgagccgg aaaccctgcg agccggcgat cagagtcctg aacggcgcgg
    17161 ggcgaggagg ccgacgccgc ggcgccggaa ccgcgagata aagacgacgc gcgagccgcc
    17221 ggccagccgg cgcgccgccg ccgacgccgc ggcgtgagaa gccgcaaacg ccacgatgga
    17281 ggaaccgctc cccgcaagca gaggatgagt gagaccgtga gctgaaattt cctcccgcag
    17341 ggtgttatag cgcaggtcgg tgtccgatac aagggtagag aaagtattat acatacattt
    17401 ttgcaaagcg atccagtcgc cgcgctcgat ggcgtcgatg gccgcggcca ggccgtggcc
    17461 gtcgtgccaa tcggcaggcg tgatgcggcg gtaggcggcc gccgtgctgc tggggaagcc
    17521 cggccagacg ccgacgatcc agcgcggtgg gaggcggttg cggatcgggc ggagctgttc
    17581 gccgcggccg gacccgacct gggttccgcc gcgaatgaag aaggggcagt cgctgcccag
    17641 gtcggccgcc agttcctcca gggcatgggc gggcaggcgc aggtgataga gccggttgag
    17701 ggcgaccagc gtcgccgcgg cgtcggcgct gccgccgccc aggcccgcgc ccggggggat
    17761 gcgcttgatg aggtcgaccg cgagaccgcc gacccggccg gggaaggccg cacgcaggcg
    17821 ttcccaggcg cgagcgatca gattgccggg tccgagcggc acgccgggat ggtcgcaggc
    17881 gatgcggcag acgtcctggt ccagcgggcg gcaacgcagt tcgtcgcccc agttgagggc
    17941 ctgaaagatc gtatccagat cgtgaaagcc gtcgggccgg cggcggcgca ccgcgaggta
    18001 ggggttgaat ttggccgggg caaacaggcg cagggcggtg gccggcgggg cgctcatggg
    18061 aggggttcct cgggcggcag tgggcggccg gcgacgagcg ccgcggcgtg atcgagcagc
    18121 gtgcgggcgc gggccgcgac gctgaggcgc tcgagggcga gacggccggc ctgccgggcc
    18181 agatgctcgc gcagggcggg gtcgtccgcg gcgcgaccga tcgccgcggc cagggccggt
    18241 gggtcattga gcggcgcgag cagagcggtc tcgccgtcgc ggacgagatc ggggacgccg
    18301 ccgacgcggg tggagacgac cgggacgccg gtttgcatcg cctcgagcag gacggtcggc
    18361 aggccctcgc tgcgcgacgg gatgaccagc agatcgagct gggggtagat cgcggcgggg
    18421 ttgtcggtaa agccggccag ccggacccgg tcggcaaggt tggactcggc gatgagtcgg
    18481 cgacaggcgt cgcgcagcgg gccatcgccg acgagaacgg cgcgcaggtg cgggcgcccg
    18541 gcggcggcca gcgccagcgc gcggatgaag atgtcggggc ctttttcggg cgagagccgg
    18601 ccgatgcagc cgacgaggaa atcggccggg gcggcggcgc cgaggtcgcg gcggaggcgg
    18661 gcgtcgcggg gggcggaggg gagcggggcg atcgcgttgg ggacccagcg gatgcggtcg
    18721 gtctggacgc cgcggcgggc cagctcgcgg ccgaaggggc gtgagacgac gacaacctcg
    18781 tcggcgcggc gcagccagcg ggcgtcgagc gcgtgataga gccgcacgcg ccagttttcc
    18841 caggtgaaac cgtgaaagac ggcttgccag cggagggcgc cgcgccgcgc ccggcagccc
    18901 aggaaactgc ccttatagcc gtggctctgg tagaggtcgg gctggaagcg gtcgagcagc
    18961 cgcaggaact ggggaacgat ggccggatcg aagcggaagc gctcgcggag gatttcggtg
    19021 ggaaggtcgg cgtcggccat ggcgcgggta aaggcgttgt cgtgcgcggg cgcgccgcgc
    19081 accaggccca ccatgacgat ctggtggccg aggcggcggg catcgacggc gaagtcgcgc
    19141 gccaggcgcg cgggcccgct caggtgatcg gtgtcgagga agatggcgat gcgcatgcgg
    19201 ccctgcgacg cgggttgcga gcagctcccg gagggggagc agcggaacgg gggtcaggat
    19261 agcacgccgg gggaaaaaag gtagggaggg gatggggcgg tggggacggg gtggatgggg
    19321 gacggggttt tcgggagccg aggacgaggg aggagagccg agagccaaat tccggggagg
    19381 ccgttggggg cggggacgag gacgaaaggc gaggggcgat tgctgggggt cagaaggtgg
    19441 tggggaggca gtcttcctga tcgtcgtcgt tgtcgagttc gtcgctcagc tccatgatcg
    19501 gctttttgcc gcccaggggg agggtgaacc agacgcgggt gcccttgccc gagccggggc
    19561 tgtgcaggcc gatcgtgccg cccatcagtt cgaccaggtt gcgggtgatg gcgaggccga
    19621 ggccggttcc ctcgtagcgg cgggtggagc tgccgtcggc ctgcgtgaat ttctcgaaga
    19681 ttttctgttg ctggtcggga gcgatgccga tgccggtgtc ctcgacgacg atcagcaggc
    19741 tgccggcctg ttcggggtcg acctcggccc agacgcggac ggcgccttct tcggtgaact
    19801 tgatggcgtt gccgacgagg ttgatcagga tctgcttggt gcggtggaag tcggcgtggg
    19861 cgagcaattc ctcgggatcg gcggcgcgga cctcgaagga gaggtgcagg ccggcgcggg
    19921 tcgcctggac gctggcgagg ctttcgacct cgcgcaggag tttttcgacc gggaagtcgt
    19981 cgatcaggaa attcatgcgg ccggcctcga tgcgggcgat gtcgaggacg tcgttgatga
    20041 gctggagcag gtgctgggcc gaggcgaggg cgttggcgat gtattcgcgc tcctcctcgg
    20101 ggctttcgca caggccctcc aggatcaatt ggaggaagcc aatgatcgag ttgagggggg
    20161 tgcgcagctc atggctggta ttggcgagga attcactctt ggtctgatcg gcgcgcgcga
    20221 gcgcctcgtt catctgttcg agctgttcga gcgaggcgtt cagctcgtcg ttcttgcgcc
    20281 ggagcagttc ctggctggcc gccatcgtcg ccttgagttt cgccagctcg atgtcgcgtt
    20341 cggtctggtg cgagatgtcg cggaagaaga agccgaggcc gtcgccctgg gggtgaaccg
    20401 agtattggaa ccggcgggtt tcgccctcgg cgatgtaggc gaacgtcagg cggtcgggat
    20461 tcgtatcctc gtcgcgtgcg gcgaggacct ccttgagcga ggtatcggcc agcaggggaa
    20521 agaggcgctg gagggtgagg ccgaggacct cgccggcgga gcggtgcagc gtgcgcaggg
    20581 cgcggctgtt ggcgtaggag atgtggccgg tgccgtcgat cgcaagataa ccttcgctga
    20641 tgatctccag cagcgattcc atgcaggccg cgcgggccgc gcgcgcgagc gcctcgtcgg
    20701 ccgggccgga ttcggtctcg ggtttgagcc aggccagcag gagcgggcgg ctgccggcgg
    20761 gatcgcatcc agccagccgc aggcggcagg catgcatggc gccgtcggcg tcgaggacgc
    20821 ggacaccctc gagcaccggg acatgttccg ccgtgcccga gagcatcccg gccatccggc
    20881 tgaagaccgc ccgctgcgaa tcatcgttga tcaattggag gatggggcag ccgatcaggt
    20941 cgccgcgcga gatgccgagg agttgcccgg cggccaggtt cgcgtcgacg acggtcaggg
    21001 agcggccgtc gaggacaagc aggggctcct cgccgtggcg gaaggcggcg tggagataac
    21061 catcgctgac cagcaggtgc ggcgaggatg agaagggaga ggacatggaa ctcccgcgat
    21121 gaggcacgac gaagtgcatc gcgcccgact gtggcgcgac gggtcgcgcc ggtggaaacc
    21181 agaagcgacc gatcgcgcaa tcgccgcgcg acgggttctc taccttttat cgtttgatcg
    21241 gcgcgggagc ttgagcaatc gggggcggaa aaggaggggt tcagggttcc gccggagccg
    21301 gggcgcgggc gcggatcggc ccggctcaga gagccggctg ccgcggcttc acggggactt
    21361 cccagacgct gcccccgggg taatgaatcc ggaccgtttt cacgtcctcc atcccttcct
    21421 gacgctcata gcgggcctcc acgctgctga ccacctgggc gcagatcccc gcgggggccg
    21481 agacgatcaa ctccagtgga agcgccgaga gccggcctgc cggggtttga gccgccgcca
    21541 ggttggcttc atggcacggg gtcggcacct gcacccggcc ggacacgatc aggcgggggg
    21601 agccgtcgtc agtttgctca agccatgcgt cccaggtgtc ggtggggtgc tcggcggccg
    21661 gacgcgccgt ttcgtaatcc ccggggggag attcgcggtc gccggcgcgg gggctccggc
    21721 accccagcag caggcagagc gccaggggga caacgacagc catcgcgggc agcattctcg
    21781 gccgcatggt tcgattctcc tcgctcacca gaattttata ccatggggcg cccatcctgt
    21841 agttaattgg cacgcggtgc ggcggggccg acgcgaagca catcagcgga gatgatcatg
    21901 aggaatgtgg agagccggat tgcatcgcgc ggggggatgc tgtttgacgc ggccgagctg
    21961 gcggcgctga ccaccggggg cgacgggctg ttccgcgcgg gggaaaacgg cgtccgcgcc
    22021 atccttgagc cgaaggacgg caaggcggag ttcatcgtat tcgaagacgg ccgcgtcctc
    22081 gcccacatcc gctcgcggat gggttatccc gcattctatc cgctgcatcc ggcgcggatc
    22141 gagcggcccg tcgaggcggt cctgatggat ctggacggga cgagcgtgcg cagcgaacac
    22201 ttctggatct ggatcattga gcagacaacc gcccggctgc tggggaaccc gaagttcgag
    22261 ctggaggagg cggatctgcc gcatgtgtcg ggccattcgg tcagcgagca tctgcgccac
    22321 tgcattgcca aatacgcgcc ggagaagacg gtcgaggagg cgcgccgcca ctatttcgac
    22381 atcacgcgcc acgagatgaa tgaaatcatg gaagggcggg gcaaggacga cgccttcaca
    22441 ccgacggagg ggctgaagga attcctgctg gagctgaagg ggcgcaagat ccggatcggg
    22501 ctggtgacct ccgggttgta tgagaaggcc tggccggaaa tcctggcggt gttccggcaa
    22561 ctcgggctgg gcgatccgcg ggagttttac gacgcgatca tcacggcggg gcatgcgatc
    22621 cgcaagggcg aggccgggac gctgggcgag ctggagccga agccgcaccc gtggctgtat
    22681 gcggaggcgg cgcgggtcgg gctggggatc gagttcggcc ggcggcatca cgtcgtcgga
    22741 atggaggaca gcggggcggg ggtgatctcg atccggctgg cggggttcac ttgcctcggg
    22801 gtggcagggg gcaatatcgc gtcgagcgga accggttcgt tttgttcgcg ggtgtgccgg
    22861 gggttggagg aggcgttggc ggagattgtg tgaacgggaa acctagtctc aagtttcagg
    22921 cacgcgtccg tcgcgtgcgc caccgttgaa atccgtccga tcggccggat ccgtccgatc
    22981 ggccggattg cttctttccg gatcaggctt ccggattgcg gcgggtcatg atgcggtgcg
    23041 agccggcgag catcaggcgg cgcgggacgc aacgaagggc ggcgaggagc aggcggttcc
    23101 accagccgtc gaccatgaaa gcgcgccgtc ccaacaacat aagcgaggac tcaaccacct
    23161 gttccggttc gcgattgaag cggccgacgc tcttgatgcc cgccccggcg ttgaattcgg
    23221 tgcgggtcat gccggggcag acggcgagga cgtcgatgcc gaggtcgcga tactcggcgt
    23281 ggagcgcctc ggcgaggagc agatcgaagg ccttggtggc gctgtagacg gaatagaacg
    23341 gcgtggggat gaagcccagg acgctggaga cgatgatcag gccgccgcgg cggcgccgga
    23401 ccatcggcgg gaggaaccgc agagtcagat cgaggacggc gcggcagttg gcatcgacca
    23461 tcgccacctg gcgcgccggg tcggtctgat cgaggcggcc gtagaggcca aacccggcgt
    23521 tattgacgag caggccgatt ccccaaccca gttcggcgac gcggcgctcg atccgctggc
    23581 cggcgtcggg ggccgccagt tcctgctcga tgagctcgac gcggacacgg tggcgttggg
    23641 tgagttcgtt gcgcagctct tcgagccggt cgcggcggcg ggccgtgatg accagatcga
    23701 agcggcgcgc cgccagggcg cgggcgtatt cgcggccgat gccggaactg gcgccggtga
    23761 cgagggcggc ggggccgtgg cgtgtggggt caagcggcat gggaggaagg ggacttcagg
    23821 gaaatcaggg acccggggag ccctgaatcg tgttcgcgcg cgttacagtt tgcccttggt
    23881 cgagagg
//
LOCUS       DAOPCO010000035        22838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2401_length_22838_cov_5.470746, whole genome shotgun sequence.
ACCESSION   DAOPCO010000035 DAOPCO010000000
VERSION     DAOPCO010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 22838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22838
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_22838_cov_5.470746"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..911)
                     /locus_tag="PLS90_04510"
     CDS             complement(186..911)
                     /locus_tag="PLS90_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01700.1"
                     /translation="MTSIPTPAARRRPPRGGILMTFVILLLVVMGVAGTVMWNRTREE
                     KVIINRRAQTEPKMMLDRYFVLLGVRHRASDPDWQELLDFMSRDDRLWLDENRATLAR
                     LDPDLNGRNPLHLKPEAQTWSAVHALLDFGSHKVRPVVSTLRVNGVHAVAYIHDPGRI
                     DTIRPIFLVQESGYWKLRRFGGARDSEPVMRLLVESKSTSGLPLTAEDQAWQRNPAAY
                     EQQMQDALLREAGVDPNAQVQAN"
     gene            complement(908..1135)
                     /locus_tag="PLS90_04515"
     CDS             complement(908..1135)
                     /locus_tag="PLS90_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01701.1"
                     /translation="MSNSSHKTDLSLNRRIWLLWACGWLILVLGVLFVAWRGPRWLER
                     IARDEPDPLRREKLDNLVEVRRELDREDGSQ"
     gene            1338..2165
                     /locus_tag="PLS90_04520"
     CDS             1338..2165
                     /locus_tag="PLS90_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPK01702.1"
                     /translation="MDLSPTVLKQYMKDISRHQVMSKQEEYDLFARLRSGDESARDEI
                     IQCNLRLVVRLAQRFQGKGLSLEDLIQEGNIGLLEVIDRFDHTLGFRFSTYAAFWIRQ
                     AIQVAVRKQGSLIRLPVRKARALGYMSEVIQEFRSTKGRAPTYAEMAERLGLQEEQLE
                     ELVQISKTALSLDSPLDEDGGTLQERVVDERTPLASQQTMRNELIRKVHQALAQLSER
                     ENRVLQMRFGIHGGRPRSLRSISRRVGLSQEGVRRVEQRALAKLARPHMRTQLSGLI"
     gene            3113..5038
                     /gene="pilM"
                     /locus_tag="PLS90_04525"
     CDS             3113..5038
                     /gene="pilM"
                     /locus_tag="PLS90_04525"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPK01703.1"
                     /translation="MAVVTSNCLGLDIGSHHIRIAYLEMGRSGPRIVQLLEAPLEVEE
                     GVPEAQRFQQIGKQVQEMLREARIRTKNAVFSIPGQSVFVRRIKLPRTSEERMQRIIR
                     YEARQQIPFPLDKTIMEYQVFEEEDSPEANVLLVAIKREFILNFMRMVRRTGLRAVGI
                     SVSTLALFNFHEANSSDRPLTLRSPRELERERRAAAAERRKQEKGQDKKRKKLKGKQG
                     EGDQAAEEALIEPEEDALEAMGFEEIQAYVNLGSSLMDIAIPKPGPARMIGFPRSVPL
                     AGNEMDRAVKDRLNLESLEAARKLKEEQVAILSTEYEMQGEEGRYNRQASEAATAVAD
                     RMINELRRSLDFYISQPDGVAVDSLVLSGGLARMPFLAGYIEEKMGLPVELAELKNPN
                     LRPPDTPPESFAPYAVAVGLALQGLRVAQSQIDFLPEDIKNIRGIQERRWEGVALVAM
                     LFVVILLNLGVGGSLITRYNQQSEAIQQTMVAAQRDSQVVNAATGRFTKVNDAYEQLA
                     KAVGWRDYWLLFLKGFIERRPADILIDELHMRADGAVIIRGRSPNLSSVQSMLDRLNP
                     DNWAGASWGIELGSTSEVFDPTFGTRVHRFEVVMKTLVRGTRVRTMGQNPVNPNAGNT
                     NYNRNMGAFGFGGAMPR"
     gene            5063..5572
                     /locus_tag="PLS90_04530"
     CDS             5063..5572
                     /locus_tag="PLS90_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01704.1"
                     /translation="MADTPNAFMASLKKNVHLWFAYGLGLLLLIRLGLYLMMAGYAGE
                     PVPDPQPASFPPIDTAGDTYTKVEKMLDPWPPFENSEYYPIATYNIFDPKTVQDAGAL
                     EQRANEKYNQAYIAYQQRNLAGALQYVNEALQIAPNHRAARQMLEKLNAEMQPAATDG
                     ASSPTAGGG"
     gene            5921..9274
                     /locus_tag="PLS90_04535"
     CDS             5921..9274
                     /locus_tag="PLS90_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01705.1"
                     /translation="MKTAVSACRAIGATLALILLNGPAFTQVVGQQAVWGRRAARAPQ
                     ATAAWQEQDLRQRLGRVGLDSAAAGSARLVAQANEFQAIPLPPDQGQMLPGGEPTTRT
                     LAVPAVVDEAMPARAARPEPMATDGLALPPPPEATTLDAPPAEILVDDITRIQVNTLV
                     KRAERLAADGDLAAAQEALDKAFDLWPESPEAHKVAEMVPGLRKPQPVLREAELNPKP
                     PIMTRMFEEPQTLDGELGIMHRRAVELYNQGDVRGARAIWHQMNEIDPENPVAVTWLE
                     QTESAMMQIMADEDRRTQVEQLQTQAEVLLNSPITISTDRNVSLPEFMYNLSFTTPIE
                     LQYYIADGADAPIYANFVNKPLHEVLDTVLGPAGLRWRIDEKNVITIEPDLQSRTYRL
                     TPSQVDQLRALQNSGQLQQIVWGRPDKPSSAVDITLDERANMLIITGSQLHIERIESW
                     LHSASGEATTPDLVTAFYKINPQDGPKIKSLINSIIKTDPDADPFDVEKKILVDGEDL
                     IVHETPENIRLIEELLLNQDFIQQLRDEELDLANFNLIPRDWEKVDEEFIANVVEMIE
                     VFLYGREGRSAAEAQGRRLTFDDATLTLSIIDTPSRLEDVGTYINSLPQFQSESQQEI
                     VFLKYQIAEELAANLERILNLTSTGGAFGTGGSEIVMQLRRGEEQTFQNARFRAIEFQ
                     GATTGSQDDDRYDQEVRLQVIYGGQVNETSIRELTTTQVEDFEITGEDITANENGNGR
                     ARLRIRYIPQLSEGIRFEDERLAQQLLDRVDPLEEDGISIEPFGGLNALIIRYNNLPL
                     YQQAKDLIVQLDQPIRQVEIDTKFVEVNETRAKEFSSDFNIAGLGRGRDIDWSNQMIN
                     TKFAQDIDEFRDVFSPPVENPLNANLIKGTTVFNAIIGNFPNIQYTLRLLEAEAIINI
                     VNGPKVLALDGEEAEFRIETYVPQQNLTQGNQTLTRVFNAFENAGNELEFADQDEVDN
                     QGLVSGVVLRVTPEITSADSIILSELSAELIDFEGWLADIIEPVVRDVADLTEDQFFT
                     PIVAPTATSNYSQRMVQKRKKILTDARISNGGTIVIGGWTGERSQELTSGVPVLRNMP
                     YFGKLLFSRNQTTKDRTTLLIFLTCLIIE"
     gene            9534..12347
                     /gene="polA"
                     /locus_tag="PLS90_04540"
     CDS             9534..12347
                     /gene="polA"
                     /locus_tag="PLS90_04540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPK01706.1"
                     /translation="MHASDDKTLYLIDGHSLTFKAYYAVRGLTNARGEPTGAVFGFLR
                     MLLKLLDDSSPQYLAVVFDTGKPTFRNELYDAYKANREAPPPDFGVQMGWVLQLLQAM
                     GIAVYSMDGFEADDLIGTMARTMAEQGRAVIVSADKDLLQLVTDRVHLLRPGLNETIL
                     YDPTRVREQLGIRPDQMIDWLALVGDSSDNIPGVPGIGQKTATTLLQEYDTLERLLAR
                     AGELKRPKQRQALLENAEQARLAQRLAAIDCDVPLVWSPADCRLPENLFSPEAAALMA
                     ELGFHSLLREKGIEPARVAAAAPAAAHPPETRLYASLTEEAELRAWVEGAMQAEWLAL
                     DTETTSTDSVTCRLVGISLCHEEGKAVYIPVGHRIPCPPRGNAAQAAPEAGNDQPEMA
                     LDFEEDALLESQLPIATIRRLLEPLLAGRGPRLAGHHAKFDWKVLRQAGFNPAPPAFD
                     SMIASYLLDPDKATGHGLKTLGKEICGCEMRPITELIGTGRGQVTIDRVALDDVRDYA
                     CLDADITFRLRRELGRRLDEHPRLRKLMDELEMPLVEVLIDVELGGFTVDPTCLNRLG
                     GELAQHLADLSRRIWEAVGHPFNIGSPRQVAQVLFEELGLKPGKKLKTGYSTNEAELE
                     RLARDHPVARLILEHRGYEKLKSTYVDSLPKLVNPKTGRIHTSFHQTIAATGRLSSTD
                     PNLQNIPIRSEMGRAIRRAFVADGPQCLLLKADYSQIELRILAHISRDPALLAAYREG
                     RDIHRQTASEVFGVAPEQVDREMRSRAKVINFGIIYGMSPHGLAQQLEISRVEAAEFI
                     DRYFNTYPGVRRWIEELLAEARRTGYVETLMGRRRLVGDLNQRNRMVREAAERVAVNT
                     PIQGTSADMIKRAMILIHRRLNEVAPGARMVCQVHDELVFSVPRDQLKPAEGFIREQM
                     TGALPLAVPVEVEIHCAPNWAEC"
     gene            12351..12956
                     /gene="coaE"
                     /locus_tag="PLS90_04545"
     CDS             12351..12956
                     /gene="coaE"
                     /locus_tag="PLS90_04545"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPK01707.1"
                     /translation="MSFILGLTGTFGSGKTTAGGMLRELGAELIDADELARQVVEPGE
                     PALAEIAAQFGAEMIRPDGALDRRRLADRVFADRRAMARLNQIIHPRVRELERKRLGE
                     LADRPLVVLDVPLLYETGMDRMCTQVAVVRITENQRFARLRRRGFNEQAIIARLGMQM
                     AQARKVKLADHVIDNSGSLDETRNQLQRLLEEIRQTRMKAP"
     gene            13274..14581
                     /gene="rho"
                     /locus_tag="PLS90_04550"
     CDS             13274..14581
                     /gene="rho"
                     /locus_tag="PLS90_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPK01708.1"
                     /translation="MESSPEGDERETVVVGPDNEVNIDALKHMRIGELYQIAKHMNIE
                     NYSSLRKQDLIFKILQTNAEKKGSLYGAGVLEILPDGFGFLRSANYNYLPGPDDIYVS
                     PSQIRRFNLRKGDTITGHMRPPKEGERYFALLKVDTINFEQPDKTQDVILFENLTPLY
                     PESMIKMETQPENFTGRVMDLMDPIGKGQRALIVAPPRTGKTMILQNIARGITANHPE
                     VYLIVLLIDERPEEVTDMERTVEGEVVSSTFDEPAERHTQVAEMVIEKAKRLVERKRD
                     VVILLDSITRLARAYNTTAPPSGKILSGGIDSNALHKPKRFFGAARNIEEGGSLTIIA
                     TALIDTGSRMDDVIFEEFKGTGNMELHLDRKLVDRRVFPAIDINRSGTRKEELLLPQS
                     TLQRVWLLRKILNELNPVEAMEFLLERLRKTDSNDDFLATMNQ"
     gene            14649..14936
                     /locus_tag="PLS90_04555"
     CDS             14649..14936
                     /locus_tag="PLS90_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HPK01709.1"
                     /translation="MPLRFEWDQEKARSNFRKHGITFAEASTAFGDALSRTIPDPLHS
                     EDEQRFALIGQSLSGRLLVVVHTHRAERTRSISARRASNKERQQYEKNARR"
     gene            14914..15123
                     /locus_tag="PLS90_04560"
     CDS             14914..15123
                     /locus_tag="PLS90_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01710.1"
                     /translation="MRKMPDDEDLLEEYDFTDGEQRKYARLYAEGTNVVVLDPDVAEV
                     FPDHESVNDALRHLAAVIKRQKAKP"
     gene            complement(15129..16010)
                     /gene="rsmA"
                     /locus_tag="PLS90_04565"
     CDS             complement(15129..16010)
                     /gene="rsmA"
                     /locus_tag="PLS90_04565"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPK01711.1"
                     /translation="MPAYPRVDFLLLQLRLKQIHQSKRLGQHFLTDDALLGRIADAAG
                     AAPDTLAVEIGPGPGVLTTHLAARAGGVLAIELDRRLEQLHAESFGIYPEIRFHYADA
                     LRVNLSELAERRRAELGLARIVLAGNLPFQITSPFLFAQCGPGVPWRRMALMVQKEVA
                     QRVVSPPDRKDYGILTVKLATWWRVVERFAVPAGRFQPPPRVDAAVLVFEPQPPPVDA
                     AEAAAWWPGFSEFVDAAFAQRRKMLVNSLAARWQLFPGRERANAALARLGLDPRIRAE
                     ALSPGELARLHGHLARA"
     gene            16288..17808
                     /locus_tag="PLS90_04570"
     CDS             16288..17808
                     /locus_tag="PLS90_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01712.1"
                     /translation="MFSSLIHRLLRIGGALLLGCLATFAGAQVTDPVTEADAPPEPPV
                     IDDPITTDDQAPPVELLPYEESAQELDVDLDVPAQPPSPTEILDPSREPELEERMEPV
                     VQSPVENRPIEIFDPGKGEELTPIQRAALSQAQRRAALERRSLADIQNLVTYEYELAG
                     TSEQEILREAMVRSLKSAAGRLYFGNYYLLGLDLLDPYLRRHGRPFIASVDVLERRFL
                     PNDQIWLHVRMSVNLDDFYKELDAKQFIARPNLLPNVGVMIHEIIEGQPDPTGQARVR
                     VERALEENLLQTYSERMRQPDTNTDASATPMLLQQARLEAQRQDIDVLITGTVTVTEV
                     PLQQIYTIEAFQQGDIFYDRYTFREVKADLDMYRVDNGELLLSLTDRYPAAADTARDA
                     MNAALDELLIRMTQRFADHLREYWTATMAYKGEDYRVMVSGVAPEQIPGIYGLVQSLD
                     PEVKVYEKSFYGGVLVFNVVLPERAPLDLEGFLRQSHEPQFVVNRVGRRQLQLEVL"
     gene            complement(17765..18595)
                     /locus_tag="PLS90_04575"
     CDS             complement(17765..18595)
                     /locus_tag="PLS90_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015279068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPK01713.1"
                     /translation="MSLPSPPPPDIEVKSVSFRYGKQWVLEDVDLTVTARDFVGLIGP
                     NGGGKTTLLRIILGLLRPDRGQVRLFGLPPAEGCRGIGYVPQHIRFDFQFPISVMDVV
                     LMGRLRHAPRLGRYKTRDRELAEQALEQLGVIDLRRRAIGELSGGQRQRVLIARALAG
                     EPRLLALDEPTSSVDGRVEREVFEMLHALNERITILLVTHELGFISSYVKTVACLSRK
                     LVCHPTSGVTGDVIEELYGGHVHMVRHDHVGHGTDCVFERGEIASKEPRAATDGDRPG
                     "
     gene            18949..20751
                     /locus_tag="PLS90_04580"
     CDS             18949..20751
                     /locus_tag="PLS90_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01714.1"
                     /translation="MSKRSHILSWMRGLTLGALLLALIGCGESTTAPKTTGAESGAGE
                     AAPPAAEAPAAREGIAAPALPAANIELPAPPALADPQLLGMVRVREPNQVLDRLGAWA
                     ELFQPGMNAAALRAQFQMQSGIDPTQMTPGGNLATYIWAPSQEMPGPPTVGAIVPVAA
                     ISPTSPYAADSAAHGDYSSVGLGPQGLQAVEQNVEALHAAAQAPTAADVELMANVTEI
                     LDIYGDQLRSMTQMAGGMLMMSGDPNAQRFAPILQAELTALVDFLEKLARAMITIDFD
                     PAHLELGLFFQAEADSGLFDAGAVEQRDLMALLPADSPVRVQMTLTDPRAFAETYAKY
                     IKLAFPGVTVQQSAQIDQLVEDLGQFDQMVFAQSLRLESTSGSQGPQPKMIVHSVIEV
                     DDAEALMEFVREKITIINEGMLHDLYTSMGMTMTISPEPEVREVEGVSVDHYALKVDV
                     DETMFPAEEAARMEAMVGDLSFDVARFGDLVLYSMGAPIDGFLTSLKQTVAVTPSQSM
                     QAFQGGGIFYGEVNPAALLEMLRAVMPADQAAQIPAINPDTPSLLMAGFHRDQKAYYR
                     LRAPRALLETLRTQPATDDLTTGGDAPPLGEPAE"
     gene            20807..22321
                     /locus_tag="PLS90_04585"
     CDS             20807..22321
                     /locus_tag="PLS90_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01715.1"
                     /translation="MSPSSRRRGRVLVLNRWAAPGCAALLIFFTCGYFQNSRPGWNVN
                     SQFGLTAAIAERGTLRIDLYHNRPETETGDKAFFEGHYYSDKSPVTAFLGVPAFWAYA
                     AAVKAMGRAVDYDAARYWVTWLTIGAAAAVLAGLLTGMLQSEGVPPARAARAGALWIA
                     ATPLLTYAILFFNYLPACAMALGGLLLTAPAWRPDAAAPACTRLLLGGLLLGLAAWTL
                     NTMALAALVVAVGLAAGLIRRERSAGGVARAFAPWALGCLLGVAGQMGYTLALFGEVA
                     SPYAYEADPFFREQMARGFMGATRPRAWVAWLVTFHPFQGLFLWFPIVLVALLGVFAR
                     LAGKRAGRTDALIALAIFAGLLLYNSAYFMWWGGWAYAPRHLIPALPFLASGLAGWLG
                     SQRRAVRRAVLGLALIGAVFNVAALALDPQPPPGLLQEELMAPEMVDAWPSPFLALQR
                     YVWLGNQTDNNWGTALGLTGWASLVPLWIVWGVALILFGRLDGPRRGAAERAAP"
     gene            22318..>22838
                     /locus_tag="PLS90_04590"
     CDS             22318..>22838
                     /locus_tag="PLS90_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01716.1"
                     /translation="MTARLRAARLPSQTIPCPYLAGLEARFTDYLLVEYDAEALSQLL
                     EHGYRRFGRHLFRPACPGAPGASWCGACLPLRVPTARFQPSRSQRRVLRRGADVELRI
                     GRPVYSEEKYEIYRRHKQRFPRPDAAGEGAGDRGELAGEGAEDEAQRRESFRFSFYDG
                     NPFTQECEYRLEG"
BASE COUNT         4143 a         6824 c         8165 g         3706 t
ORIGIN      
        1 cgtcgacgac cgctgcgcgg tctctgccgc cgcactccac aaaatgacgt cgggcgaggg
       61 gagagggcga tggcgatact gaagcggcgt cgacgaccgc tgcgcggtct ctgccgccgc
      121 actccacaaa atgacgtcgg gcgaggggag agggcgacgg cgatggcgat ggcgatcagg
      181 ccgagtcaat tcgcctggac ttgggcgttg gggtcgacgc cggcttcgcg cagcagggcg
      241 tcctgcatct gttgttcgta ggcggccggg ttgcgctgcc aggcctggtc ctccgcggtc
      301 agggggaggc cgctggtgga tttggactcg acgagcaagc gcatgaccgg ttcggaatcg
      361 cgggcgccgc cgaaccggcg gagtttccag tagcccgatt cctgcacgag gaagatcggc
      421 cggatggtgt cgatccggcc cgggtcgtgg atgtaggcga cggcgtggac gccgttgacg
      481 cggagggtcg acaccacggg gcgcaccttg tgcgagccaa aatcgagcag ggcatggacg
      541 gcggaccagg tctgcgcttc cggcttcaag tggagcggat tgcggccgtt gaggtcggga
      601 tcgagccggg cgagggtcgc gcggttctcg tcgagccaga gccgatcgtc gcggctcatg
      661 aaatcgagca attcctgcca atccgggtcg ctggcgcgat ggcggacgcc cagcagcacg
      721 aaatagcggt cgagcatcat tttcggctcg gtctgcgccc gccggttgat gatgaccttc
      781 tcctcgcgcg tgcggttcca cataacggtt ccggcgacgc ccatgacgac cagcagcagg
      841 atgacaaagg tcatgaggat gccgccgcgc ggggggcgac ggcgcgcggc gggtgtcggg
      901 atgctcgtca ttggcttcca tcctcccggt ccagctcgcg ccggacttcg acgaggttat
      961 caagtttttc ccggcggaga gggtcgggct cgtcgcgggc gatccgctcg agccagcgcg
     1021 gcccgcgcca ggcgacgaac aggaccccca ggaccaggat caaccagccg caagcccaga
     1081 ggagccagat ccgccggttc agggagagat cggttttgtg cgaagaattg ctcattgcgg
     1141 gttcctgcac aatcgggtgc actctttttg tgcagatgaa cttcggcgga gcgtcggtga
     1201 ccgacggccc cgacttttcc caagccttgg attgacttga tttgaggctg atcccgggtc
     1261 gtccatccct cccgccgacg gtttggaagc taagttgctc taccgccttc tagctgttat
     1321 tcggaaagga tgtttcgatg gacctgtccc ccaccgtcct gaagcagtat atgaaggata
     1381 tcagccgcca ccaggttatg tccaagcagg aagaatatga cctgttcgcc cgcctgcggt
     1441 cgggcgatga gtcggcgcgc gatgagatca tccagtgcaa tctgcggctg gtcgttcgcc
     1501 tcgcgcagcg gtttcagggc aaggggctct cgctggagga tctgatccag gagggcaaca
     1561 tcggtctgct cgaagtgatc gaccggttcg atcacacgct gggcttccgg ttttcgacgt
     1621 atgccgcgtt ctggattcgc caggcgatcc aggtggccgt gcgcaagcag gggtcgctga
     1681 tccggctgcc ggtgcgcaag gcgcgggcgc tgggctatat gtccgaggtg atccaggagt
     1741 tccggtcgac gaaggggcgg gcgccgacgt atgccgagat ggccgagcgg ctcggcctgc
     1801 aggaggagca gttggaggaa ctggtgcaga tcagcaagac ggcgttgtca ctcgattcgc
     1861 cgctggacga ggatggcggg acgctgcagg agcgcgtcgt ggacgaacgg acgccgctgg
     1921 cctcgcagca gacgatgcgc aatgagctga tccggaaggt gcatcaggcg ctggcgcaac
     1981 tgtccgagcg cgagaaccgg gtgttgcaga tgcggttcgg gattcacggg ggtcggccgc
     2041 gcagcctgcg cagcatcagc cggcgggtgg gtctgagcca ggaaggggtg cggcgggtcg
     2101 agcagcgggc gttggcgaag ctggcgcggc cgcacatgcg gacgcagttg agcgggttga
     2161 tttgatctat catggtttcc tcggggcccc cacggggcca cctggatcgg gccgggcgcc
     2221 tctagagaga gtgatgcgaa agcgtcaggg ggccactctc gaagacggcg tccggcctgt
     2281 tcgttttact gtcctctctg tcgggatcgc ctggatcggt tcggccgtcg ccacgggact
     2341 ccgtggcgat gggtatcgcg gttcccgcca ccgattcgcg gactaaatcg gtcaaatttc
     2401 atccctcgtt gtggctcgtc atcgtggtcg ttcatctgat cgtgagggca ggagagacgg
     2461 agcggacatc ggggacatga gggacggaag gggcggaagg cgtggccgtg agagcgagag
     2521 gctggaagga aggggaattc gagagccgag gacgagggcc gagggccgag agccgaattt
     2581 cgagaagggc ctttgggggc gagggcgagg acgagggacc gagacgaggg acgaatatcg
     2641 ggggggcagg cgcaacccaa accagaccgc ggaggggtag aaaaggtttg actaatgggg
     2701 aatggtccga taaaaagcga gggtcgatca gcggttaggg gcctaagaaa actaggcttg
     2761 cgccatttgc aggatcgaac tcggccgcgc acccgcacca tggcccgcca gcgccattgg
     2821 ttgccgtcgg ggcgtctcat ccgcgccaga ggaaccacca tttcggcgaa acgcaacagg
     2881 agttgaccct cagtcggccg gacccctcgc cgcgcggcga gccggcggcc gaagccgtgg
     2941 gatggaacag gcaaggaagc gtccgccgat ggggaaaaac ccacccgtgc aattcaggga
     3001 aagtcgtctg cttgacgatc tgtcggcgga gcgtcacaat cgctcgcttc gtaaccgggg
     3061 ggtgaacccg ccggcctact cgcgcgcgac agagcaaagg tgacccgcag gcatggcggt
     3121 tgtaaccagt aactgcttgg gcctggatat cggctcgcac catatccgga tcgcctacct
     3181 ggagatgggc cgcagcgggc cgcgcatcgt gcagttgctc gaggccccgc tggaagtgga
     3241 agaaggggtc cccgaggcgc agcggtttca gcagatcggc aagcaagtgc aggagatgct
     3301 gcgcgaggcg cgcatccgca cgaagaatgc ggttttctcg attcccggcc agtcggtgtt
     3361 cgtccgccgg atcaagctgc cgcgcacgtc ggaagagcgg atgcagcgga tcatccgcta
     3421 cgaggcgcgc cagcagatcc cgtttcccct cgacaagacg atcatggagt atcaggtctt
     3481 cgaggaggag gactcgcccg aagccaatgt gctgctggtg gcgatcaagc gcgagttcat
     3541 cctgaatttc atgcgcatgg tgcggcggac cgggttgcgc gcggtcggca tttcggtctc
     3601 gacgctggcg ctgttcaact ttcacgaagc gaacagttcg gatcggccgt tgacgctgcg
     3661 ctcgccgcgc gagttggaac gcgagcggcg ggccgcggcg gccgagcgcc gcaagcagga
     3721 gaagggccag gataagaagc gcaagaagct gaaggggaag cagggcgagg gcgatcaggc
     3781 ggcggaggag gcgctgatcg agccggagga ggatgcgctc gaggcgatgg gcttcgagga
     3841 gatccaggcg tatgtgaacc tgggttcttc gctgatggac atcgcgattc ccaaaccggg
     3901 accggcgcgg atgatcgggt tcccgcggtc ggtgccgctg gccggcaacg agatggaccg
     3961 ggcggtgaag gaccggctca acctggagag cctggaggcg gcgcgcaagc tgaaggaaga
     4021 gcaggtggcg atcctctcga ccgagtacga gatgcagggg gaggaagggc ggtacaaccg
     4081 gcaggcgagc gaagcggcga cggcggtggc cgaccggatg atcaatgaac tgcgccggtc
     4141 gctcgatttc tatatatcgc agcccgacgg cgtggcggtg gacagcctgg tgctgtcggg
     4201 cgggctggcg cggatgccgt tcctggcggg ctacattgaa gagaaaatgg gcctgccggt
     4261 ggagctggcc gagctgaaga accccaatct gcggccgccc gacacgccgc cggagagttt
     4321 cgccccctat gcggtggcgg tcgggctggc gctgcagggt ttgcgcgtcg cgcagagcca
     4381 gatcgacttc ctgcccgaag acatcaagaa catccgcggc atccaggaac ggcgctggga
     4441 aggcgtcgcg ctggtcgcga tgctcttcgt cgtgatcctg ctcaatctgg gggtcggcgg
     4501 gtcgctgatc acccgctaca accagcagag cgaggcgatc cagcagacga tggtggccgc
     4561 ccagcgcgac agccaggtgg tcaatgccgc cacgggccgg ttcacgaagg tgaacgatgc
     4621 gtatgagcaa ctggccaagg cggtgggctg gcgggattac tggctactgt tcttgaaggg
     4681 atttattgag cggcgtccgg ccgacatcct gatcgacgag ctgcacatgc gggccgacgg
     4741 ggcggtgatc atccggggcc gatcgccgaa cctgagcagc gtgcaatcga tgctggaccg
     4801 gttgaatccg gacaactggg cgggcgcctc gtggggaatc gagttgggca gcacgagcga
     4861 ggtgtttgat ccgacgttcg ggacgcgggt gcatcggttc gaggtggtga tgaaaacgct
     4921 ggtgcggggg acgcgggtgc ggacgatggg ccagaatccg gtcaatccca acgccggcaa
     4981 taccaactac aaccggaaca tgggggcctt cggctttggc ggggcgatgc cgcgctgagc
     5041 gctgggaatt gagggtaacg gcatggcgga cacacccaat gcattcatgg ccagcttgaa
     5101 gaaaaatgtg cacctgtggt tcgcctatgg gctggggttg ctgctgctga tccggctggg
     5161 gttgtatctg atgatggcgg gctacgcggg cgagccggtg ccggacccgc agccggcgag
     5221 ttttcccccg atcgacacgg cgggcgacac ctatacgaag gtggaaaaga tgctcgaccc
     5281 gtggccgccg tttgagaaca gcgagtatta cccgatcgcg acgtataaca tcttcgaccc
     5341 caagacggtg caggacgcgg gggcgctgga gcagcgggcc aacgagaagt acaaccaggc
     5401 gtatatcgcg tatcagcagc ggaatctggc gggagcgctg cagtatgtga atgaggcgtt
     5461 gcagatcgcg ccgaatcatc gggcggcgcg ccaaatgctg gagaagctga acgccgagat
     5521 gcagccggcg gcgaccgacg gggcgagctc gccgacggcg gggggcggct aaaggcggtt
     5581 tggcctggag cccgcgcggt ccagtgccct gaaaccatgc aaaaagccat ggttttactt
     5641 gacaaaagtc cgcgcgatag cgaagaccat tgtggtgctg ttacccggaa agggtttagc
     5701 ccttgggaag gggagatccg ccgcttgacg gggggccaca aggtccgggc attccagcgg
     5761 gcgccggggg tcgggggccc gcgcggtgag acggagtgcc cggtcgtcca ccaaccacgc
     5821 tgaacgccga tgccggagat gaccctgcac ccgctgccgg catccagacc ccggcgaata
     5881 tctctaacgc agcccatgtc gaggaagtag gaaccatctg atgaagaccg ctgtttccgc
     5941 ctgcagggcc atcggggcga cgctcgccct gatcctgctg aacggccccg ccttcaccca
     6001 agtcgtcggc cagcaagctg tctggggccg tcgcgcggcg cgggcgcctc aagccaccgc
     6061 ggcatggcaa gagcaggatc tgcgccagcg gctggggcgc gtggggctcg actcggccgc
     6121 cgccggctcg gcgcggctgg tggcgcaggc caacgaattc caggctattc cccttcctcc
     6181 cgatcaaggt caaatgctcc ccggaggcga gccgacgacg cgcacgctgg cggtaccggc
     6241 ggttgtcgac gaagcgatgc cggcccgcgc ggcccggccc gaaccgatgg caacggacgg
     6301 gctggcgctg ccgcctccgc ccgaggccac gacgcttgat gcgccgcccg ctgagattct
     6361 tgtcgacgac atcacccgga tccaggtcaa cacgctggtc aagcgcgccg agcggctggc
     6421 ggccgatggc gatctggcgg ccgcccagga agcgctggac aaggcctttg acctgtggcc
     6481 cgaaagcccc gaggcgcaca aggtggccga gatggtgccg ggcttgcgca agccgcagcc
     6541 ggtgctgcgc gaggcggagc tcaaccccaa gccgccgatc atgacgcgga tgttcgagga
     6601 acctcagacg ctggatgggg aactggggat catgcaccgg cgggcggtcg aactgtacaa
     6661 ccagggggac gtgcgcggcg cgcgcgcgat ctggcaccag atgaacgaga tcgatccgga
     6721 gaacccggtg gcggtgacct ggctggagca gaccgaatcg gcgatgatgc agatcatggc
     6781 cgacgaggac cgacggacgc aggtcgagca gcttcagacg caggccgaag tgctgctcaa
     6841 ttcgccgatc acgatctcca ccgaccgcaa cgtgtcgttg ccggagttca tgtacaacct
     6901 gtcgttcacg acgccgatcg agctgcaata ctacatcgcc gacggcgccg acgccccgat
     6961 ctacgccaac ttcgtcaaca agccgctgca cgaggtgctc gacacggtgc tggggccggc
     7021 ggggctgcgc tggcggatcg atgagaagaa cgtcattaca atcgagccgg atctgcaatc
     7081 gcggacctac cggctgacgc cgagccaggt cgatcagctg cgcgcgctgc agaacagcgg
     7141 gcagcttcag cagatcgtct ggggtcggcc tgacaagccg tcgtccgcgg tggacatcac
     7201 cttggacgaa cgcgcgaaca tgctgatcat taccggttcg caactgcata tcgaacggat
     7261 cgagagctgg ttgcactcgg ccagcggcga ggcgacgacg cccgacctgg tgaccgcgtt
     7321 ctacaagatc aatccgcagg acggaccgaa gatcaagtcg ctgatcaact cgatcatcaa
     7381 gacggatccg gatgccgatc cgttcgacgt ggagaagaag atcctggtgg atggcgagga
     7441 cttgatcgtt cacgaaacgc cggagaacat ccgcctgatc gaggagctgc tgctcaatca
     7501 ggacttcatc cagcaactgc gcgacgagga actggatctg gccaacttca atctgatccc
     7561 gcgcgactgg gaaaaggtgg atgaagaatt catcgccaac gtggtggaga tgatcgaggt
     7621 cttcctgtac gggcgtgagg ggcgcagcgc ggcggaggca caggggcggc ggctgacgtt
     7681 cgacgatgcc acgctgacgc tgagcatcat cgacacgccg agccggctgg aggatgtcgg
     7741 gacctacatt aattcgttgc cgcagttcca gtcggaaagc cagcaggaga tcgtgttcct
     7801 gaagtatcag attgcagagg aactggcggc gaacctggaa cggattctga acctgacgtc
     7861 caccggcggg gcgttcggga ccggcgggag cgaaatcgtg atgcagttgc gccggggcga
     7921 ggaacaaacg ttccaaaacg cgcgcttccg cgcgattgag ttccaggggg ccacgacggg
     7981 cagccaggat gacgaccgct acgaccagga agtccggctc caggtgatct atggcgggca
     8041 ggttaacgag acgtcgatcc gcgagttgac gacgacgcag gtcgaggatt ttgaaatcac
     8101 cggtgaagac atcacggcca acgagaacgg caacggccgg gcgcggctgc ggatccgcta
     8161 catcccgcag ctttccgagg ggatccggtt tgaggatgag cggctggcgc agcagctgct
     8221 ggaccgggtt gatccgctgg aagaggacgg gatatcgatc gagccgttcg gtgggctcaa
     8281 cgcgctgatt atccgctaca acaacctgcc gctgtaccag caggcgaagg atctgatcgt
     8341 tcagctcgac cagccgatcc ggcaggtgga gatcgacacg aagttcgtgg aagtgaacga
     8401 gacgcgggcg aaggaattca gctcggactt caatatcgcg ggtctgggcc gggggcgcga
     8461 catcgactgg tcgaaccaga tgatcaacac gaagttcgcg caggatatcg acgagttccg
     8521 cgacgtcttt tcgccgccgg tggagaaccc gcttaacgcg aacctgatca aggggacgac
     8581 ggtcttcaac gcgatcatcg gcaatttccc caacattcag tacacgctgc ggctgctcga
     8641 agccgaggcg atcatcaata tcgtgaacgg ccccaaggtg ctggcgctcg acggcgagga
     8701 ggcggagttc cggattgaaa cctacgtgcc gcagcagaac ctgacgcagg gcaaccagac
     8761 gctgacgcgg gtgttcaacg cgtttgagaa cgcggggaac gaactggaat ttgccgatca
     8821 ggacgaagtg gacaaccagg gactggtgtc gggcgtggtg ttgcgcgtga cgccggagat
     8881 tacgtcggcc gattcgatca tcctgagcga gctgtcggcc gagctgatcg acttcgaggg
     8941 ctggctggcc gacatcatcg aaccggtcgt gagagatgtg gctgacctga ccgaggacca
     9001 gttcttcacg ccgatcgtgg cgccgacggc caccagcaat tactcgcagc ggatggtcca
     9061 gaagcgcaag aaaatcctga ccgacgcgcg gatttccaac ggcgggacga tcgtgatcgg
     9121 cggctggacc ggcgaacgga gccaggagtt gacctccggc gtgccggtgc tgcgcaatat
     9181 gccgtatttc ggcaagctgc tcttcagccg caatcagacg accaaggatc ggacgacgct
     9241 gctgattttc ctgacgtgcc tgattatcga ataatccagc ggaccggctg gattgcctga
     9301 tggactgaac aggcggcaag ccgcggggct tgcgccgacc gggaaccacg ccggagagcg
     9361 acggggccag ccccgcgccg gtcgtggaac gcaccggccc tctccctcgc cggcccgcgc
     9421 ccggcgcgcc gccgccaccg acggcgccgg ccgccgcccc atccgttggt tccaccgcct
     9481 gacggccagt cgattcaaac cggcgtgcgc cctgcccgcc cagccagacc accatgcacg
     9541 cctccgacga caagacgctc tacctgatcg acggtcattc gctgacgttc aaggcgtact
     9601 atgcggtgcg cgggctgacg aatgcgcgcg gggagccgac cggggcggtc ttcgggtttc
     9661 tgcggatgct gctcaagctg ctcgatgaca gctcgccgca gtatctggcc gtggtcttcg
     9721 acacggggaa accgacgttc cgcaacgagc tgtatgatgc ttacaaggcc aatcgcgagg
     9781 cgccgccccc cgatttcggc gtgcagatgg gctgggttct ccagttgctc caggcaatgg
     9841 ggattgcggt ttactcgatg gacgggttcg aggccgatga cctgatcggg acgatggcgc
     9901 ggacgatggc cgagcagggg cgcgccgtga tcgtctcggc cgacaaggac ctgctccagt
     9961 tggtgacgga ccgggtgcac ctgctgcggc cggggttgaa tgagacgatt ctctatgatc
    10021 cgacgcgcgt ccgcgagcaa ttggggatcc ggcccgacca gatgatcgac tggctggcgc
    10081 tggtgggcga cagttcggac aacatccccg gcgtccccgg gatcggccag aagacggcga
    10141 ccacgctcct gcaggagtac gacacgctgg agcgcctgct ggcgcgggcc ggcgagctga
    10201 agcgacccaa acagcgccag gcgctgctgg agaacgccga gcaggcgcgg ctggcgcagc
    10261 ggctggcggc gatcgattgc gacgtgccgc tggtctggag cccggccgat tgccgcctgc
    10321 cggagaatct gttcagcccc gaggcggcgg cgctgatggc ggaactggga ttccactcgc
    10381 tgctgcgcga aaaggggatc gaaccggcgc gcgttgccgc cgccgcgccc gccgccgcac
    10441 acccgcccga aacccggctg tatgccagcc tgaccgagga agccgagctg cgcgcctggg
    10501 tggagggggc gatgcaggcc gaatggctgg cgctggatac cgagacgacc tcgaccgatt
    10561 cggtaacgtg ccggctggtc ggcatctcgc tctgccacga agaggggaag gcggtttaca
    10621 ttccggtggg gcaccggatt ccctgcccgc cgcggggcaa tgccgcccag gccgcgccgg
    10681 aggcgggcaa cgaccagccg gagatggcac tggatttcga ggaggatgcg ctgctggagt
    10741 cgcagcttcc gattgcgacg atccggcggc tgctcgagcc gctgcttgcg ggccgcggac
    10801 cccgactggc ggggcatcat gcgaagttcg actggaaggt gttgcgccag gcgggcttca
    10861 acccggcgcc gcccgcgttc gattcgatga tcgcctcgta tctgctcgac ccggacaagg
    10921 cgaccgggca cgggctgaag acgctgggca aggagatctg cggttgcgaa atgcgcccga
    10981 tcacggagct gatcgggacg ggccgcggcc aggtcacgat cgaccgggtg gcgcttgacg
    11041 atgtgcgcga ctatgcctgt ctggacgcgg atatcacgtt ccggctgcgg cgcgagctgg
    11101 gccggcggct ggatgagcac ccgcgcctgc gcaagctgat ggacgagctg gaaatgccgc
    11161 tggtggaagt gctgatcgac gtggaactgg gcgggttcac ggtcgatccg acctgcctca
    11221 accggctggg aggggagctg gcgcagcacc tggccgacct gagccggcgg atctgggagg
    11281 cggtcggtca tccgttcaac atcgggtcgc cgcggcaggt ggcacaggtg ctgtttgagg
    11341 aactggggct gaaaccgggg aagaagctga agacgggcta ctcgacgaac gaggcggagc
    11401 tggagcggct ggcgcgggat catccggtgg cgcgattaat tcttgaacat cggggctacg
    11461 agaagttaaa atccacgtac gtggattcgc tgcccaagct ggttaatccg aaaacgggcc
    11521 ggatccatac ctcattccat caaaccatcg ccgccacggg ccgtctcagt tcaaccgatc
    11581 ccaacctgca aaacatccca atacggagtg agatgggccg agccatccgg cgcgccttcg
    11641 tcgccgatgg accacaatgt ttgctgctga aggcggatta ctcgcagatc gaattgcgca
    11701 tcctggcgca tatcagccgg gatccggcgt tgctggcggc ctaccgcgaa ggacgcgaca
    11761 tccatcgcca gacggccagt gaagtgttcg gggtggcccc cgagcaggtg gaccgcgaga
    11821 tgcggtcgcg cgccaaagtg atcaactttg gcatcatcta cggcatgagt ccgcacggtc
    11881 tggcgcaaca gctcgagatc agccgggtcg aggccgccga attcatcgac cgctacttca
    11941 acacctaccc gggggtgcgg cgctggatcg aggagttgct ggccgaagca cggcgcaccg
    12001 gctatgtcga gacgctgatg ggtcggcggc ggttggtggg ggatctgaac cagcgcaatc
    12061 ggatggtgcg cgaggcggcc gagcgggtgg cagtcaacac gccgattcag gggacgagcg
    12121 ccgacatgat caaacgggcg atgatcctga ttcatcgccg gctgaacgag gtggcgcccg
    12181 gcgcgcggat ggtctgccag gtgcacgacg agctggtatt ttcggtgccg cgcgaccagc
    12241 tgaagccggc cgaaggcttc atccgcgagc agatgaccgg ggcgctgccg ctcgctgtgc
    12301 ccgttgaggt ggaaatccat tgtgccccca actgggcgga gtgttagcgg gtgtcattca
    12361 tcctggggct gacgggcacg ttcggcagcg gcaagacgac ggcggggggg atgctgcgcg
    12421 aactgggggc tgagctgatc gacgcggacg aactggcccg ccaggtggtg gagccggggg
    12481 agccggcgct ggcggaaatc gccgcccagt tcggcgccga gatgatccgg cccgacggag
    12541 cgctcgaccg gcggcgacta gccgaccggg tgtttgccga ccggcgggcg atggcccggc
    12601 tcaatcagat tattcacccg cgggtgcgcg agctggagcg caagcgtctg ggtgaactgg
    12661 ccgaccggcc actagtcgtg ctggatgtgc cgctgctgta tgaaacgggc atggaccgga
    12721 tgtgcacgca ggtggcggtg gtgaggatca cggagaacca gcgatttgcg cggctgcggc
    12781 ggcgcggatt caacgagcag gcgatcatcg cccggttggg catgcaaatg gctcaggcgc
    12841 gtaaggtgaa gctcgccgat catgtcatcg acaattccgg gagtctggac gagacccgga
    12901 accaactcca acggttgctg gaggagatcc ggcagacgcg aatgaaggca ccatgaacac
    12961 gaagaagaac gagaacgaga gtgcgaacga cgcagggact cagcggcggc gcgggcgtcc
    13021 gcgcaagacg ccgatcggcc cgaaggcgga cctggtcagc gagatgatcg agcggagcga
    13081 ttcgctggcg cgggcgcgcc agacggcgcg ccagcaggag gcggccgagc cgcgccccgc
    13141 gccgacggcg agcccggcgc cggccgaagc gcgccacacg acggcggtgg ccgaggagcc
    13201 ggacgtcgcg cacaaccgcc cgcgcggacg gacgcggctg tcggccagcg agatcgacaa
    13261 cgagctgcgg atcatcgaat cgagccccga gggagacgag cgcgaaaccg tcgttgtcgg
    13321 gccggataac gaggtcaata tcgacgcgct gaagcacatg cgcatcggcg agctgtatca
    13381 gatcgccaag cacatgaaca ttgagaacta cagctcgttg cgcaaacagg acctgatctt
    13441 caagatcctg cagaccaacg ccgagaaaaa ggggagcctc tacggcgcgg gcgtgctgga
    13501 gattctgccc gacggcttcg gcttcctgcg ctcggcgaac tacaactacc tgcccgggcc
    13561 ggacgacatt tacgtttcgc cgtcgcagat ccggcggttc aacctgcgca agggcgacac
    13621 gatcacgggc cacatgcgtc cgccgaagga aggcgagcgc tacttcgcgc tgctgaaggt
    13681 cgacacgatc aacttcgagc agcccgacaa gacgcaggac gtgatcctgt tcgagaacct
    13741 gacgccgctc tacccggaat cgatgatcaa gatggagacg cagccggaga atttcaccgg
    13801 tcgcgtgatg gacctgatgg atccgatcgg caaggggcag cgggcgctga tcgtggcgcc
    13861 gccgcgcacg ggcaagacga tgattctgca gaacatcgcc cgggggatca cggccaacca
    13921 tccggaggtc tacctgatcg tgctgctgat cgacgagcgg cccgaggaag tgaccgacat
    13981 ggaacggacg gtcgagggcg aagtggtcag ttcgacgttc gacgagccgg ccgaacgcca
    14041 cacgcaggtg gccgagatgg tgatcgagaa ggcgaagcgg ctggtggagc gcaagcggga
    14101 cgtcgtgatc ctgctcgact cgatcacgcg cctggcgcgg gcctacaaca cgacggcccc
    14161 gcccagcggc aagatcctat cgggcggcat cgactccaac gcgctgcaca agccgaagcg
    14221 gttcttcggc gcggcgcgca acatcgagga aggcggctcg ctgacgatca tcgccacggc
    14281 gttgatcgac accggcagcc ggatggacga cgtgatcttc gaggagttca aggggaccgg
    14341 caacatggag ttgcacctgg accgcaagct cgtggaccgg cgtgtgttcc cggcgatcga
    14401 catcaaccgg tcggggacgc gcaaggaaga gctgctgctg ccgcagtcga cgctgcagcg
    14461 ggtctggctg ctgcgcaaga tcctcaacga gctgaatccc gtcgaagcga tggagttcct
    14521 gctggagcgg ctgcgcaaga ccgacagcaa cgacgacttc ctggcgacga tgaatcagta
    14581 gcgttattcc ggaacttgtg gagcggggcg ccccgaccgg cctgtgacag tcccggaggc
    14641 tttttcagat gcccttacga ttcgagtggg atcaagagaa agcccgaagt aatttccgga
    14701 aacatgggat cacctttgcc gaggcttcca cggcctttgg cgatgctctc tcacgcacga
    14761 ttcccgaccc gcttcactcg gaggatgaac agcggttcgc cttgatcggt caatcgctca
    14821 gtgggagact attggtagtc gttcacaccc atcgagccga acggacgaga agtatcagtg
    14881 cgcgaagagc atcgaacaag gaacgacagc aatatgagaa aaatgcccga cgatgaggac
    14941 ctgctcgagg agtacgattt caccgacggc gagcagagga agtatgccag gctgtacgcc
    15001 gagggaacca acgtagtcgt tctggacccg gacgtggcgg aagttttccc ggatcatgaa
    15061 tcggtcaacg acgcactacg tcatctggct gctgtcatca aacggcagaa agccaaaccc
    15121 taacagattc aagcgcgcgc gagatggccg tgaagtctgg ccaattctcc cggcgagagg
    15181 gcttcggcgc ggatccgcgg gtccaggccg agtcgcgcca gcgccgcgtt cgcgcgttcg
    15241 cgccccggaa acagttgcca gcgggcggcc agggagttga cgagcatctt gcggcgctgg
    15301 gcgaacgccg cgtcgacgaa ctccgagaat cccggccacc aggccgccgc ttccgccgca
    15361 tccaccggcg gcggctgcgg ctcgaacacg agcaccgcgg cgtccacgcg cggagggggc
    15421 tgaaaccgcc ccgccggcac ggcgaagcgc tcgacgacgc gccaccaggt cgccagcttt
    15481 accgtcagga tgccatagtc cttccggtcg ggcgggctga ccacccgttg cgcgacttcc
    15541 ttctggacca tcagcgccat gcgccgccac gggacgcccg gtccgcactg ggcaaagagg
    15601 aacggcgagg tgatctggaa cgggaggttg ccggccagga cgatgcgcgc gaggccgagt
    15661 tcggcgcggc ggcgttcggc cagttccgac agattgacgc gcagggcatc ggcgtagtgg
    15721 aagcggattt ccgggtagat gccgaagctc tccgcatgga gctgttccag ccggcgatcg
    15781 agttcgatgg cgaggacgcc gccggcgcgc gcggccagat gggtggtcag gacgccgggc
    15841 ccggggccga tctcgacggc cagggtgtcg ggcgcggcgc cggcggcatc ggcgatgcgg
    15901 ccgagcagcg cgtcgtcggt caggaaatgc tgacccaggc gtttgctctg gtggatttgt
    15961 ttgaggcgga gctggaggag caggaagtcg acgcgcgggt aggcgggcat ggagtaccgt
    16021 tcggagcgat ctgggcgggg gctcagggcc tgatccggcc gaagttgggc gacttccgcg
    16081 gggtgataaa agttgacagc ccccggcccg tggggcattg attataccat gaatgcgcct
    16141 gcgcccggca agcaggcaaa gaggggacgt atcgcccgcg gcgcggcgtc ccgccggcgg
    16201 cggcgtgccg gggcggcccg ctggccgaca ctcgccgcac agcgcgccgg gcgattccgg
    16261 cgcgcgccat gcgcggaggt tccggcgttg ttttcatctc tcatccatcg actgttgcgg
    16321 atcggcggcg cgctgttgct ggggtgcctg gccacgttcg ccggcgcgca ggtgaccgat
    16381 ccggtgacgg aggccgatgc gccgccggag ccgccagtga tcgatgaccc gatcacgacg
    16441 gacgatcagg cgccgccggt cgagctgctc ccgtatgagg aatcggccca ggagctggac
    16501 gtggatctgg acgtgccggc gcagccgcct tcgccgacgg aaattctcga cccctcgcgc
    16561 gaaccggaac tcgaggagcg gatggagccg gtggttcaat ccccggtgga gaatcggccg
    16621 attgagatat tcgacccggg caagggggag gaactgacgc cgatccagcg ggcggcgctc
    16681 tcgcaggcgc agcggcgggc ggcgctcgaa cggcgatcgc tggccgacat ccagaacctg
    16741 gtgacgtatg agtacgagct ggcggggacg agcgagcagg agatcctgcg cgaggcgatg
    16801 gtgcgctcgc tcaaatcggc ggccgggcgg ctctacttcg gcaattacta tctgctgggg
    16861 ctcgatctgc tcgatccgta tctgcgccgg cacggccggc cgttcatcgc cagcgtggat
    16921 gtgctggagc ggcgcttcct gcccaacgat cagatctggc tgcacgtgcg gatgagcgtc
    16981 aatctggacg acttttacaa ggaactggac gccaagcagt tcattgcgcg gcccaatctg
    17041 ctgcccaacg tcggcgtgat gatccatgag attatcgaag gtcaacccga tccgacgggg
    17101 caggcccgcg tccgcgtgga gcgcgcgctg gaggaaaacc tgctgcagac ctattcggag
    17161 cggatgcgcc agcccgacac caacaccgac gcctcagcaa cgccgatgct gctccagcag
    17221 gcgcggctgg aggcgcagcg gcaggatatc gacgtgctca tcaccggcac cgtgacggtg
    17281 acggaagtcc ccctgcagca gatctatacc atcgaggcgt tccagcaggg cgatatcttc
    17341 tacgaccggt atacgttccg cgaggtgaag gccgacctgg atatgtaccg ggtcgacaac
    17401 ggcgagttgc tgttgtcgct gaccgatcgc tacccggcgg ccgccgacac ggcgcgcgac
    17461 gcgatgaacg cggcactgga tgagctgctg atccggatga cgcagcgttt cgccgatcac
    17521 ctgcgcgaat actggacggc gacgatggcc tacaaggggg aggattaccg cgtgatggtc
    17581 agcggcgtcg cccccgagca gattccgggg atctacgggc tggtccagtc gctcgatccg
    17641 gaagtcaagg tctacgagaa gtcgttctac ggcggcgtgc tggtgttcaa tgttgttctg
    17701 cccgagcggg cgccgctcga cctggaaggt ttcctgcgcc agtcgcacga accgcagttc
    17761 gtggtcaacc gggtcggtcg ccgtcagttg cagctcgagg ttctttagag gcaatctctc
    17821 cccgttcaaa gacgcaatcg gtgccgtgac cgacgtggtc gtggcggacc atgtggacat
    17881 ggccgccgta gagctcttcg atcacgtcgc cggtcacccc cgaggtggga tggcagacca
    17941 gcttgcggct gaggcaggcg accgtcttga cgtaggacga gatgaagccg agctcgtggg
    18001 tgacgagcag gatcgtgatc cgctcgttga gcgcatggag catttcgaag acttcgcgtt
    18061 cgacgcgccc atcgacgctg gaggtgggtt cgtcgagggc aagcaggcgg ggttcgccgg
    18121 ccagggcgcg ggcgatcagg acgcgctggc gctggccgcc ggagagctcg ccgatggcgc
    18181 gccggcgcag atcgatgacg cccagttgct ccagcgcctg ttccgccagt tcccggtcgc
    18241 gcgtcttgta acgccccagg cggggggcat ggcgcagccg gcccatgagg acgacgtcca
    18301 tcacgctgat gggaaactgg aagtcgaagc ggatgtgctg agggacatag ccgatgccgc
    18361 ggcagccttc ggccgggggc aggccgaaca ggcggacctg gccgcggtcg ggccgcagga
    18421 ggcccaggat gatccgcagc agcgtcgtct tgccgccgcc attggggccg atcaggccga
    18481 cgaagtcgcg cgccgtcacc gtcaggtcca catcctcgag cacccactgc ttgccgtaac
    18541 ggaacgaaac cgacttgacc tcgatatcgg gggggggagg ggacggcaag gacatggcgg
    18601 cgaattcctg gacgcgggcc ggcgcggggg atgaatgggg acagccggga gcatcatagc
    18661 gtcccgcgcc aggcaatgtc atccttccac gcgagaattc gtctgttttg gaagggacat
    18721 aagggacgca agggacatta gggacaaaaa agacatgtga aggcggattc atcctttgag
    18781 gggtggcgcc gaaggcgtag tcagggtcgt taaagagctc actcgaatcg acgtctttca
    18841 tttctcgtcc ctgtgagcgc gtccgaaagt atgaacagca aaactccttt gcttttttcg
    18901 gggcggcctg tcttactaca gccgattccc attgaaagga acacgaccat gtcgaagcga
    18961 agccacatcc tgtcgtggat gcgaggcctc acgctggggg cattgctgct ggcgctgatc
    19021 ggctgcggtg aatcaacgac cgcgcccaag acgacgggcg ccgagagcgg ggcgggcgag
    19081 gccgcgccac cggcggccga agcgccggcc gcgcgggaag ggatcgcggc gccggcgctg
    19141 cccgccgcca acatcgagtt gcctgcgccc cccgcgctgg cggacccgca actgctgggg
    19201 atggtgcgcg tgcgtgagcc gaaccaggta ttggatcggc tcggggcgtg ggccgagctg
    19261 ttccagccgg ggatgaatgc cgccgcgctg cgggcgcagt tccagatgca gtcggggatc
    19321 gacccgacgc agatgacgcc cggcgggaat ctggcgacgt atatctgggc gccgtcgcag
    19381 gagatgccgg ggccgccgac cgtcggtgcg atcgtgccgg ttgcggcgat ttcgccgacg
    19441 tcgccttacg cggccgattc cgccgcgcac ggcgattact cgtccgtcgg cctggggccc
    19501 caggggctgc aggcggtcga acagaatgtc gaggcactgc atgccgccgc gcaggcgccc
    19561 acggcggccg acgtggagct gatggccaac gtcacggaga tcctcgacat ttacggggac
    19621 caactgcgtt cgatgacgca gatggccggc gggatgctga tgatgtcggg cgatccgaac
    19681 gcccagcggt ttgcgccgat cctgcaggcc gagctgacgg cgctggtcga ttttctggag
    19741 aagctggcgc gcgcgatgat caccatcgat ttcgatcccg cgcatctgga actggggttg
    19801 ttcttccagg cggaggccga cagcggcctg ttcgacgcgg gcgccgtgga gcagcgcgac
    19861 ctgatggccc tgttgccggc cgacagcccg gtgcgcgtcc agatgacgct gaccgatccg
    19921 cgagcgtttg ccgagaccta cgcaaagtac atcaagctgg ccttcccggg cgtgacggtg
    19981 cagcagtccg cccagatcga tcagttggtt gaggacctgg gacagttcga tcagatggtg
    20041 ttcgcgcagt cgctcaggct tgagtcgacg agcggctcgc agggcccgca gcccaagatg
    20101 atcgtgcata gcgtgatcga agtggatgac gccgaggcgc tgatggagtt cgtccgcgaa
    20161 aagattacga tcatcaatga ggggatgctg cacgacctgt acacctcgat ggggatgacg
    20221 atgacgattt cgcccgagcc ggaagtccgc gaggtggagg gcgtgtcggt ggaccattac
    20281 gccctaaagg tggatgtgga cgagacaatg ttcccggccg aggaagccgc gcggatggag
    20341 gcgatggtcg gcgatctgtc gttcgacgtg gcgcgtttcg gcgatctggt gctctacagc
    20401 atgggggcgc cgatcgatgg cttcctgacg tcgctcaagc aaacggttgc cgtcaccccg
    20461 tcgcagtcga tgcaggcctt ccagggcggg gggatcttct acggcgaagt caatccggcg
    20521 gcgctgctgg agatgctccg cgcggtcatg cccgcggacc aggcggcgca gatcccggcg
    20581 atcaatccgg ataccccgtc gctgctgatg gccgggttcc atcgcgatca gaaggcgtat
    20641 taccgcctgc gggcgccgcg ggcgctgctc gagacgctcc ggacccagcc ggcgacggat
    20701 gacctgacca caggcgggga cgcgccgccg ctgggcgaac ccgccgagtg aacgattgat
    20761 gacggacggc ccgccggcgt ccctatcgca tccggccgac ccggccgtgt cgccatcgtc
    20821 tcggcggcgc ggccgggttc tggttttgaa ccgctgggcg gcgcccggtt gcgccgcgct
    20881 gctgatcttt ttcacgtgcg ggtactttca gaattcccga ccgggctgga acgtcaattc
    20941 gcagtttggg ctgaccgccg cgatcgccga gcgcggcacg ctgcgcatcg acctgtatca
    21001 caaccggccc gagaccgaga cgggcgataa ggcgttcttt gaggggcact attattccga
    21061 taagagtccg gtcacggcgt ttctcggcgt tccggcgttc tgggcgtatg ccgccgccgt
    21121 gaaggcgatg gggcgggcgg tcgattacga cgcggcgcgc tactgggtga cgtggctgac
    21181 gatcggcgcg gcggcggcgg tgctcgccgg cctgctgacg gggatgcttc agagcgaagg
    21241 cgtgccgccc gcccgcgcgg cgcgcgccgg cgccctctgg atcgccgcca cgccgctgct
    21301 gacgtacgcg attctgttct tcaactacct gccggcctgc gcgatggccc tgggcggttt
    21361 gctgctgacg gcgcccgcct ggcgtccgga cgcggcggcg ccggcgtgca cgcggctgct
    21421 gctgggcggc ctgctgctgg gattggccgc ctggacgctc aacacgatgg cgcttgcggc
    21481 gctggttgtg gccgttggcc tagcggcggg cctgatccgc cgggagcgtt cagccggggg
    21541 cgtggcgcgc gcgttcgcgc catgggcgct gggctgcctg ctgggggtgg ccggccagat
    21601 gggctatacg ctggcgctct tcggcgaagt cgcttcgccc tacgcctacg aagccgatcc
    21661 gttcttccgc gagcagatgg cgcggggatt catgggggcc acgcggccgc gcgcgtgggt
    21721 cgcctggctc gttacgtttc atccctttca ggggctgttt ctgtggtttc cgatcgttct
    21781 ggtcgcgttg ctgggtgtct ttgcgcggct cgccggcaaa cgcgcggggc ggacggatgc
    21841 gctgatcgcg ctggcgatct tcgccggcct gctgctctac aacagcgcct acttcatgtg
    21901 gtggggaggg tgggcgtatg cgccgcgcca cctgattccg gcgctgccgt tcctggcgtc
    21961 gggactggcg ggctggctcg ggtcgcagcg gagggctgtt cgtcgcgccg tcctgggact
    22021 ggcgctgatc ggggcggtgt tcaacgtggc ggcgctcgcg ctggatccgc agccgccacc
    22081 cgggctgctt caggaagagc tgatggcgcc cgaaatggtg gacgcgtggc cttcgccctt
    22141 cctcgcgctc cagcgctacg tctggctggg gaaccagacc gacaacaact gggggacggc
    22201 gctggggctg acgggctggg ccagcctcgt cccgctctgg attgtctggg gcgttgcgct
    22261 gatcctgttc ggccggctgg atggcccgcg gcgtggggcg gcggagcggg cggcgccatg
    22321 accgcgcggt tgcgggcggc gcggctcccg tcccagacga taccgtgccc gtatctggcc
    22381 ggcctggagg cccgctttac ggactatctc ctcgtggagt atgacgcgga ggcgctgagc
    22441 caattgctgg agcatggcta ccggcgtttc gggcgtcatc tgtttcggcc ggcgtgcccg
    22501 ggcgcgcccg gggcgtcctg gtgcggtgcg tgcctgccgc tgcgcgtgcc gacggcgcgg
    22561 ttccagccgt cgcggagcca gcggcgggtg ctgcggcggg gcgcagacgt tgagcttcgg
    22621 atcggccggc cggtttacag cgaagagaaa tatgaaattt atcgtcgaca caagcagcgc
    22681 tttccgcgtc cggacgccgc cggggaaggc gccggcgatc gcggcgaatt ggccggcgag
    22741 ggcgcggaag acgaggcgca gcgtcgcgaa tccttccgct tcagctttta cgacggcaac
    22801 ccgtttacgc aggagtgcga ataccggttg gaggggag
//
LOCUS       DAOPCO010000036        22212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2486_length_22212_cov_4.842397, whole genome shotgun sequence.
ACCESSION   DAOPCO010000036 DAOPCO010000000
VERSION     DAOPCO010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 22212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22212
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2486_length_22212_cov_4.842397"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLS90_04595"
     CDS             complement(<1..591)
                     /locus_tag="PLS90_04595"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HPK01717.1"
                     /translation="MMAKVNEDKVAPATEETPETARTATADPAQTPAGRKPIQISRDD
                     LTEEGRKKIEEQAKRPIPDFEYTLAHQENKEGSRLVLTFEVAREVFLGEQERLLSDLA
                     KEVTLPGFRKGRAPLKLLQLRLGDDVVRDTVGSIATNVLRQEQAKQQYELLVNPQVID
                     YQEPDGAQGVLKLEIELELQPKVEPRQYTGFTVEVEE"
     gene            complement(728..1438)
                     /locus_tag="PLS90_04600"
     CDS             complement(728..1438)
                     /locus_tag="PLS90_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01718.1"
                     /translation="METLGTLSIVIPAYNERATIEKIVERVRAVELPGIAKEIIVVDD
                     CSRDGTREVIDALAERYSEVRALHHEVNQGKGAALRTGFANCTGDWVVIQDADLEYDP
                     VEYRALLEPILQGQADVVYGSRFLGSGAHRVVYYWHYMGNRFLTTLSNMFTNINLTDM
                     ETCYKMFRREILQQIQIEESRFGFEPEITAKVARLKCRIYEVGISYHGRTYEEGKKIG
                     WRDGVRAIVCILKYNLLR"
     gene            1692..2822
                     /locus_tag="PLS90_04605"
     CDS             1692..2822
                     /locus_tag="PLS90_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01719.1"
                     /translation="MTESNPENPHANPPGPVPSRRRLLAFRAVVLVAILAVGWVGGEL
                     LLALLHLEPTYVAPELHPGRLPARIALDRELLFRFVPRSRNDLNNLGYRDRDFGAKSA
                     GRRRVLMVGDSFVMGVSTTSHETIPRRLETFLGSPWEVFNMGVYGDGPDQTLLRLKRD
                     GLKLAPDQVILGLFPANDFGDLMKNLLLEPAPGGGVRRTGNDILSRELPRSRVAMHLR
                     HLLTPHYMDPAREQSIFQALMGDAYLSLEMQSAPLREHLTALMREILAEFRRLADEHG
                     FRFAVVVIPPVQAFGEDPRAVKDPVPPEQAVSNDPVVLELCAELGIDALYLLEALAAE
                     GGAELYDPIDVHLNAAGSRVAAQAIQARFFADATSPPPTSSP"
     gene            complement(2768..3976)
                     /locus_tag="PLS90_04610"
     CDS             complement(2768..3976)
                     /locus_tag="PLS90_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01720.1"
                     /translation="MRILLLSQVFPYPPYDGNILPIHHFLRCLAPRAQFTLLTREPAD
                     AGLRSEALALWESWGVRARLVRCPVRSGAAQAWACLRGGRPWINRFFSPELAAAAAEL
                     LARERFDLIQSEGVLSAQHLPAAPPARRLLVARDCLSLAHWRAWRAGHSPREALGAIK
                     IAQMERTLYRRADRILAISPTDAEAIRRLAPSARIEVLPNGVDLDQFRPRPELEQPGV
                     VAFTGAMDFAPNVEAAVWFGREIWPGAVRRVPSARLRIIGRDPVPAVLALAADPSIEV
                     TGRVERMEEEAARAQVIVSPLRRGTGIKNKVLEGAALARALVVSPVSLADIDLRPGRE
                     LELAESAGEFIEQTASLLSDPARRRALGQAARSAVEQRYGWSAMAERLWGHYGELVGG
                     GDVASAKNRA"
     gene            4333..5418
                     /gene="prfB"
                     /locus_tag="PLS90_04615"
     CDS             4333..5418
                     /gene="prfB"
                     /locus_tag="PLS90_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPK01721.1"
                     /translation="MRQRVDEARRYLRFDEMRQRIDQMEAESARPDFWDNAQAAQEHM
                     RDLNRLKNQIEPWEQLARDLSDAIELNEMAQGDPDMLAELVRQADTMEHTLESLETRA
                     LLKDQDDDKSAYLYIHAGAGGTESCDWAQMLLRMYSRWCEKEEYAVETLDLIDGEEAG
                     IRSATLLVKGEFAYGMLKAEAGVHRLVRISPFDAAKRRHTSFCSVDVWPQVDDEIQIE
                     IREEDLRIDTYRSSGAGGQHVNKTSSAVRITHEPTGIVVACQNERSQHQNKAVAMKML
                     RAKLYQKARAEQQAELDARQEAKKDISWGNQIRSYVFQPYQMVKDHRTGHETGNVSAV
                     MDGAIDGFLEAYLRWSAGMGAATPQSA"
     gene            5495..6535
                     /locus_tag="PLS90_04620"
     CDS             5495..6535
                     /locus_tag="PLS90_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK01722.1"
                     /translation="MKSPLSKWLGLVLLLAAGIAVGYLLGARTPDNAFAGDALARAEP
                     AASAPATLPTDPAPSASLSDRAPVEVAAAEPLSGDETPTAEAATSPAGEPAAAPGVEL
                     SAAIKAHAGEKISRGSSSTRKIVLTFDDGPHPSRTPQVVRILKEAGIPANFFLVGEMV
                     ESYPEQAKLVADSGFELGTHSWDHPNLTRKNEAEVRRQIVETQDLVERTTGIRPVLFR
                     PPGGNVDQTVRDICREENLVICMWSIDPRDWASGATGEKIRQKVVGDAHGGAIVCMHD
                     TKAPTIDALPGMIADLKAAGYEFTTITQLLEEKAAEDAALAASGGVIPSGGDAAPQAE
                     PLVISLEESGSY"
     gene            6642..7964
                     /locus_tag="PLS90_04625"
     CDS             6642..7964
                     /locus_tag="PLS90_04625"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPK01723.1"
                     /translation="MSTTDPVIWIFSTGTEILQGHATDRNSPTISGRLLEAGLPTARH
                     VAIADRADALQEALTEASARADLIITTGGLGPTGDDLNRHVVAAVWGRALVEDPDSLE
                     RIRARYQRRGRVMPESNRVQALIPAGAEVLPNENGTAPGFYLEPGPDGPRATLIALPG
                     PPREMAPMLDKEALPRILRRFGVERVRFRAINYHTACIAESAINELVHDLFAYDERVN
                     FALLAKMSLVDIRLTLMARDEAANDELQARWEALVRERLGEYNIFGCDNDTLESVVGG
                     LLRERRQTLTTAESCTGGRLAGRITDVAGSSEYFREGFITYANEAKMARMGVMPDLLA
                     QFGAVSAEVAEAMATGAREVAGTDWAVSITGIAGPGGGSPDKPVGLVYLGLAGPDGIV
                     HSRRHHFLGDRADVRHQAVVAALDLLRRGLLGAPLDPVLPAQAVRQDR"
     gene            8121..8978
                     /gene="thyX"
                     /locus_tag="PLS90_04630"
     CDS             8121..8978
                     /gene="thyX"
                     /locus_tag="PLS90_04630"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPK01724.1"
                     /translation="MAHITIPEAEALLDQEIKVLDKGFVRLVDYLGGDARIVQAARVS
                     YGAGTKTVREDRALIHYLMRHRHTSPFEQVILTFHVKLPIFVARQWIRHRTARLNEMS
                     GRYSVMKDEFYLPDPEQIRPQSKSNKQGRDADALDAEQARRVLEILTSAQETAYAGYR
                     EMIDLDIARELARVNLPLSLYTEWYWQIDLHNLFHFIQLRADEHAQYEIRVYAEVLGD
                     CARAVAPLAYEAFEEYVRHARQFSREEMEALRAMLAGQPCPIEQGRRRVEFFQKLGLD
                     DPALQPQEA"
     gene            9031..10317
                     /gene="hisS"
                     /locus_tag="PLS90_04635"
     CDS             9031..10317
                     /gene="hisS"
                     /locus_tag="PLS90_04635"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPK01725.1"
                     /translation="MTTFAPPRGMRDFYPEDFRARDALFSAWRETARLHGFEEYDAPV
                     VETMELLERKAGEEISDQIYTFVDKSDRRLALRPELTPSLARMIIARQGALSFPLKWS
                     AIGQCFRYERMTRGRKREHYQWNLDIVGEESVLAEAEVIGAAVAALRRLGLSANEFQV
                     RVGSRAVLAELFARSGIGQEHFMACCLALDKRGKISDDEVARLLEEEGLATRDIERVF
                     ELLAITTLDQAAAAVGADSAALAQIRELFSLAGPLGFAPFLTFDLAVIRGLGYYTGIV
                     FEAFDTAGKLRAIFGGGRYDSLLSNLGGQKMPCVGLGFGDVVVMELLADCGKLPATAR
                     RVDFAIGFMDDPSRDLALRAALAVRAAGKSCDLALAAEKPKKFFARADRAGALHAIYI
                     GPDEVQSGTFSIKPMDDRRPWTASLDSFAERLAGPA"
     gene            10554..11987
                     /locus_tag="PLS90_04640"
     CDS             10554..11987
                     /locus_tag="PLS90_04640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01726.1"
                     /translation="MARVMIIGYGPLPRTGMPYIGAAALRTRHLLKPILDAGHTVNLY
                     TLPIPATEGTEGEVSSMVPDTYEGLTYQRFTNHSAEFAVRMLTEQVRQLEPDALVGVN
                     TYPAYIACLLPTALPVWADLNGYWMAEMQGRCWIEEDDSMLADAWAIERAIVRRCDKF
                     SAVSRPQLHAVLGEMAAVGRLNRHTFDYQFGHHLPNACYRWQVTAGEGSAAGEPLLRG
                     PVVPEDAFVLLWSGGFNVWADVATLVRACDLLMQNHPNVHFVSTGGRIEGVVTRVYDD
                     FVALVEQSPYKDRYHLLGWIESDKLPAIHREADVGLNVDSRNYETMFGARNRINAMAI
                     EGLAVATTIGTEISEWLDDGHAVIAAPMGDPEALAYSIAPWIMDRSQLKVFAQKAKRI
                     MEEDFSYERTTRGLVQWLEAPALAPDNQAKISSRGGRVADLNTVAVNSLEEEALLLAR
                     HRLPEIQDALAQARAREESPRKRFRFW"
     gene            complement(12327..13280)
                     /gene="hemH"
                     /locus_tag="PLS90_04645"
                     /pseudo
     CDS             complement(12327..13280)
                     /gene="hemH"
                     /locus_tag="PLS90_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002809532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
     gene            complement(13368..14144)
                     /gene="surE"
                     /locus_tag="PLS90_04650"
     CDS             complement(13368..14144)
                     /gene="surE"
                     /locus_tag="PLS90_04650"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPK01727.1"
                     /translation="MHIVLSNDDGIEAPGLAAMADALNQIGDVTIVAPDIERSGMGHA
                     ITVFKDMLFEPHHRDGKLWGYGLAGTPADCVKIALTLLCKNRPVDLVVSGINHGQNAG
                     VNVLYSGTVAAAREAAILGFPAIAVSQLYHDPDKLTFETSARVGLEVARRVLERGLPP
                     GVMLNVNVPPVPYDAIKGWAVTRMGNSGYEDLFTCPEEADSERIIYRNIGKGWNPSTH
                     PDDGNDDVALYQDYVSITPLQFDLTAYDFLDELHAWFHAE"
     gene            complement(14157..15293)
                     /locus_tag="PLS90_04655"
     CDS             complement(14157..15293)
                     /locus_tag="PLS90_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPK01728.1"
                     /translation="MVSRREAHRTVLCEPDITPSLIQSTAAGPARFRLSTPSAASTLS
                     NPPKKLRTLVGLDKLRAVLPLRFRPGHHPPIDSRRIFPALSALPFQRLVIHGVGLLGG
                     SLGLAVRRQRLAHRVVGLGRSAEKLARARDLGALDEFQTDPAAALAGADAVVLAIPPV
                     QIRESFAQIAPYLERGTFVTDVGSVKARIVEAAEANLPSHARFVGSHPMAGSEKTGAH
                     HARADFYVGYPCLLTPTGRTAPEALALARALWEAVGSRVVELDPARHDRLLAGISHLP
                     HVVAAALIQTLTRHLAPAAELGGLCGQGLRDTTRVAGGDTEIWRQILTENAEEVLACL
                     DQMEDVLHEWRAALREAAGHPRRVATLWDEARRARQGLEATDEA"
     gene            15416..16471
                     /locus_tag="PLS90_04660"
     CDS             15416..16471
                     /locus_tag="PLS90_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HPK01729.1"
                     /translation="MSELRQNLATREWVIIATERARRPHEFTDPARQLTHERPAHLAA
                     CPFCPGQEYETPAPVLRWPAEGPWRLRVFPNKYAALSVNGEKSYALEGVRRKLTGVGY
                     HEVLVESPLHNMSPACQSPRALTQTLRAFQTRGRVLSEDPRIEQIFYFKNHGPGAGTS
                     LEHPHCQLLALPMVPHHVRMRIESQRAYVDDNAVCPYCVMMQRELIDGERIVGRNRYF
                     VALVPFAATSPFEIWIIPRRHGPTFLDLSLNELEHLAELMRLLFGKLYHGLGDPDYNY
                     IIHSAPLRDSASRYLHWYMSIVPRVTKAAGFELGSGMYINPALPEESAEFLRNQDPGQ
                     PLEDEEDEPVDQLRQSS"
     gene            complement(16450..17607)
                     /locus_tag="PLS90_04665"
     CDS             complement(16450..17607)
                     /locus_tag="PLS90_04665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01730.1"
                     /translation="MLLLVQLVIVGLCAWLVLSGWLNVVDQRAERRRDARLPPLPEDA
                     PFISVCIPARNEEHNVGPLLESLARQAYPCYEVLLLDDCSEDRTAEIALAIAARYPHL
                     RVLQGRPLESGWIGKPHACRQLAAAARGDWLLFTDADTEFARGTLTMAMRRARARHSD
                     LLSGLPRLVAVTIWERLSVPLLQTMALGGIPFRWMERLNHRRAAAASGAFILIRRAAY
                     DAVGGHEAVRGEIVDDLNLARAVKRAGFRTHLTDVTRHVRCRMYRSRAEVWEGFSKNF
                     HAVFPGYQTLLAIACLTGVFILPPVCFILSPWLGLSPWSGTILPAIQIAAVGLHRALV
                     EVHAGEGKWGTLLLTPLSAAFTIAIALRSLSRRLLRQPTPWRNRHYELWRN"
     gene            17750..18628
                     /locus_tag="PLS90_04670"
     CDS             17750..18628
                     /locus_tag="PLS90_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01731.1"
                     /translation="MPHQMTALRRRSWLIPATVLLALAFGAILAPWIAPHNIRRLETL
                     RLADSGLPPAWLAAPPAGEPAPRTPFWLGTDAQGRDVLSAILFGLRVSLLVGMVGTLL
                     ALTAGTLLGLAAGYWRGWVDGAIMRLADVQLSFPSILIALFLMAVWGQGLLKIILAVA
                     IAHWVIYARVVRGAVLAEREKDYVTAIRALGAGAPRIVLRHILPNLAAPILVISAVEF
                     ASVVMLEATLSFLGLGVPITRPSLGMLINFGFQDVFGGTWWTWVFPGLALVLLVLCLN
                     WLADILRVRLMPEGEA"
     gene            complement(18776..18982)
                     /locus_tag="PLS90_04675"
     CDS             complement(18776..18982)
                     /locus_tag="PLS90_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HPK01732.1"
                     /translation="MATGRVKFFHDKKGWGFIECEAKSDVFVHYSDIVGDGYRTLVKG
                     EFVQFELNEGPKGDKATNVVRLGM"
     gene            19604..20608
                     /gene="gap"
                     /locus_tag="PLS90_04680"
     CDS             19604..20608
                     /gene="gap"
                     /locus_tag="PLS90_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPK01733.1"
                     /translation="MAVKIGINGFGRIGRLVFRAALESDGVEVVGINDLAPTKSLAHL
                     LKYDSNHGTLRQEVGFDDQHIIVAGKKIPVFQQKDPAALPWGKVGAQIVIESTGLFTK
                     RADAAKHIDAGAKKVIISAPSGDADAMFVMGVNDKTYDPAKHTVVSNASCTTNCLAPM
                     AKVLHDHFGVVNGLMTTIHSYTNDQPSQDQIHKDFRRMRAAAVSMIPTKTGAASAIGK
                     VIPDLNGKLDGYAMRVPTPNVSVTDLTLNLSKPATAAEINAALKQAAEGELKGILGFE
                     EDELVSIDFNHCPLSSIIDGPSTRVIDGTMAKVLSWYDNEWGYSLRCIDLAKKIGASL
                     "
     gene            20778..21530
                     /gene="tpiA"
                     /locus_tag="PLS90_04685"
     CDS             20778..21530
                     /gene="tpiA"
                     /locus_tag="PLS90_04685"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPK01734.1"
                     /translation="MRRPIVAGNWKMNKTPSQARALLEELKPLVADAPCDVVVAPPFV
                     CLERAAQTLAGSAIGLAAQNVHWEPSGAFTGEVSIEMLQDLGVRFVIIGHSERRQYFG
                     ETDETVNRRAKAVLAAGLTPIICVGEQLQERESGQTGAVVSRQVRGALAELSAAQVAG
                     SIIAYEPVWAIGTGKVASTEQAQEVHALIRKLLGELYGAATADQVRIQYGGSMKPDNA
                     AGLLAQPDIDGGLIGGASLKAADFAAICNAAR"
     gene            complement(21614..>22212)
                     /locus_tag="PLS90_04690"
     CDS             complement(21614..>22212)
                     /locus_tag="PLS90_04690"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK01735.1"
                     /translation="LQTEDTPFHPRSPYAIAKLYAYWIVVNYRESYAMYCVNGILFNH
                     ESPIRGKEFVSRKVTDGVARIKLGLNERLHLGNLDSQRDWGFAGDFVEAMWRMLQQPE
                     PKNYIVATGETHTIRELVEVAFRRVGIEDWEPYVFIDPAFKRPAELYTLRGDNSLAQR
                     DLNWQPRVRFKELVEMMVDADLKRVEAEIASGRRPTGG"
BASE COUNT         3736 a         7668 c         7094 g         3714 t
ORIGIN      
        1 ctcctcgact tccaccgtga agccggtgta ctggcggggc tccaccttgg gctgcagttc
       61 cagctcgatc tccagtttca gcaccccctg ggcgccgtcc ggctcctggt aatcgatcac
      121 ctgcggattg accagcagct cgtattgctg tttggcctgc tcctggcgca gcacgttcgt
      181 cgcgatcgag ccgaccgtgt cgcgcaccac gtcatcaccc agccggagct gaagcagctt
      241 gagcggcgcg cgccccttgc ggaagcccgg cagcgtcact tccttcgcca gatcgctcag
      301 cagccgttcc tgctcgccca ggaaaacctc gcgcgcgacc tcaaacgtga ggaccagccg
      361 cgagccttcc ttgttctcct gatgggccag cgtatactcg aaatccggga tcggccgctt
      421 ggcctgctcc tcgatcttct tgcgcccctc ttcagtcagg tcgtcgcggc tgatctggat
      481 cggtttgcgg cccgcgggcg tctgcgccgg gtcggcggtg gccgtccgcg ccgtttcggg
      541 cgtctcctcc gtcgccggcg cgaccttgtc ttcattcacc ttggccatca tggggtcacc
      601 tgctgtcctg acttcgttag ggtgcagccg cgcggcggcc gatcactctg accggacctt
      661 gccgggctca aaccatccca ttctagggac gcgttgttga ttttcaagcc ctttcgggtc
      721 ggcgcgatca gcgcaacaga ttgtatttga ggatgcagac gatggcccgc acgccgtcgc
      781 gccagccgat tttcttgcct tcctcgtacg tccgcccgtg atacgaaatg ccgacttcat
      841 agatccggca cttcagccgg gccaccttgg ccgtgatctc cggctcgaac ccgaaacgcg
      901 attcctcgat ctggatctgc tgcagaatct cgcgccggaa catcttgtag cacgtttcca
      961 tatccgtcag gttaatgttc gtgaacatgt tggacagtgt cgtcaggaac cggttgccca
     1021 tgtagtgcca gtagtacacc acccggtggg cgccgctgcc gaggaaccgc gagccataga
     1081 cgacgtcggc ctggccctgg aggatcggct cgagcagcgc gcgatattcc accgggtcgt
     1141 attcgagatc ggcgtcctgg atcacgaccc aatcgccggt gcagttggcg aagccggtgc
     1201 gcagcgccgc ccccttgccc tggttgacct cgtggtgcag cgcgcggacc tcgctgtaac
     1261 gttcggcgag cgcatcgatg acttcgcgcg ttccgtcgcg cgagcagtca tcgacgacga
     1321 tgatctcctt ggcgatgccg ggcagctcga ccgcgcgcac gcgctcgacg atcttctcga
     1381 tcgtggcgcg ttcattgtag gccgggatga caatggaaag ggtgcccagg gtttccacgt
     1441 ggcgcattcc agggaagcgt gcggttcggg ccgaggccga cggtgattaa agaatgcgga
     1501 gggccaaatc gcaacttgaa tcccggcgcg gggggggccg atcttgagac gaagagcggc
     1561 gattttggat cgaccgaagc ggccgggatg gccatagtgt cgttgcgcgg cggcgcacga
     1621 acgcgccccg cgcggccggt ctcgaaccat tgaggctgga tttgccgagc gatgcccgga
     1681 atgcccccgc gatgaccgaa tcgaatcctg aaaacccgca cgcaaatccg cccgggcccg
     1741 taccctcgcg ccgccggctg ctggcgttcc gcgcggtcgt gctcgttgcc attctggccg
     1801 tcggctgggt cggcggcgag ctcttgcttg ccctgctgca tctcgagccg acgtatgtgg
     1861 cgcccgaact gcaccccggg cggctgccgg cgcggatcgc gctcgatcgc gaactcctgt
     1921 tccggttcgt cccccgctcg cgcaatgacc tgaacaacct gggctatcgc gaccgggact
     1981 tcggcgcgaa atcggccgga cgccgccgcg tgctgatggt cggcgattcg ttcgtgatgg
     2041 gcgtcagcac cacgtcgcac gaaaccatcc cccgccggct ggagaccttc ctgggctcac
     2101 cctgggaggt attcaacatg ggggtctatg gcgacgggcc ggatcagacg ctgctgcggc
     2161 tgaagcgcga cggcctgaag ctggcgcccg atcaggtgat tctcgggctg ttccccgcca
     2221 acgacttcgg cgacctgatg aaaaacctgc tgctggagcc ggcgccgggg ggcggcgtgc
     2281 ggcgcaccgg caacgatatc ctgagccgcg aactgccccg ctcgcgcgtc gcgatgcacc
     2341 tgcgccatct gcttacgccg cactacatgg atccggcccg ggagcaatcg atcttccagg
     2401 cgttgatggg cgacgcgtat ctttcgctcg agatgcagtc ggcgccgctg cgcgagcacc
     2461 tgaccgcgct gatgcgcgaa atcctggccg agttcaggcg cctggccgac gaacacggtt
     2521 tccgcttcgc ggtggtcgtc atccctcccg ttcaggcatt tggcgaggac ccgcgcgccg
     2581 ttaaggaccc cgtcccgccc gagcaggcgg tctccaacga tccggtcgtc cttgagctct
     2641 gcgcggagct tggcatcgac gcgctttacc tgctggaggc gctcgcggcc gaggggggcg
     2701 ccgagctgta tgacccgatc gacgttcacc tgaacgccgc cgggagccgt gtggccgccc
     2761 aggcgattca ggcgcgattc ttcgccgacg cgacgtcgcc gccgcccacc agttcgccgt
     2821 agtgccccca tagccgctcc gccatggcag accagccgta acgttgctcc acggccgaac
     2881 gagcggcctg gcccagcgcc cggcgccgcg ccggatcact cagcagcgac gcggtctgct
     2941 cgatgaattc cccggccgac tcggccagct ccagttcccg cccgggccgc agatcgatat
     3001 ccgccagact gacggggctg accaccagcg cccgcgcgag cgcggcccct tccagcacct
     3061 tgttcttgat gcccgtccct cgccgcagcg gcgagacgat cacctgcgcc cgcgccgctt
     3121 cttcctccat ccgctccacg cggccggtta cttcaattga tgggtcggcg gccagcgcca
     3181 gcaccgcggg gacggggtcg cgcccgatga tccgcagccg ggcggagggg acgcggcgaa
     3241 cggcgcccgg ccagatctcg cgtccgaacc agacggcggc ttccacgttc ggcgcgaagt
     3301 ccatcgcgcc tgtgaacgcg acgacgccgg gctgctccag ttcgggtcgc gggcggaact
     3361 ggtccagatc gaccccgttg ggcaacacct cgatgcgggc cgacggcgcg agccgccgta
     3421 tcgcctcggc gtccgtcggc gagatggcga ggatccggtc ggcgcggcgg taaagcgtgc
     3481 gctccatctg ggcgatcttg atcgcgccga gcgcctcgcg gggcgaatgg ccggcgcgcc
     3541 aggcccgcca atgcgccagc gacaggcagt cgcgggcaac gagcaggcgc cgcgccggcg
     3601 gcgccgcggg caggtgctgc gcgctcagga ccccctcgga ctgaatcagg tcgaatcgct
     3661 cgcgcgcgag cagttcagcc gcggccgcgg ccagttccgg actgaagaag cgatttatcc
     3721 acggccggcc cccgcgcagg caggcccacg cctgcgccgc gccgctgcgg accgggcagc
     3781 gcacaagccg cgcccgtacg ccccagcttt cccatagcgc cagcgcctcc gaacgcaacc
     3841 cggcgtcggc tggctcgcgc gtcagcagcg tgaactgggc gcgcggcgcc aggcaacgca
     3901 ggaaatgatg aatcggcagg atgttgccgt cgtacggcgg gtaggggaag acctgcgaga
     3961 gtaggagaat acgcaccgga tcgccccatc gactgattcc cgccgattta accgggcggg
     4021 ggaagaaatg ggaagaggga acatgaagga gggggggtga cgaaggacgg ggggcctgtg
     4081 aatctgcgat ttacaacaaa gcgagaaaga tcggggtggg ccgttggggg cgattacgag
     4141 gacgagggcg agtgacgaga gacgagcgat ccgggacggg tggcgtcttg caaaggggcg
     4201 ggggtgttcg atatgatacc agcgtccttt tttggtcggc gcgagcgccc ggcgcggcct
     4261 cgccaccccc gttcaaggag cccgacggca ttgcccatca cccaggaaga cttgcgtcct
     4321 tcgctcgatc gcatgcgcca gcgtgtcgat gaggcgcgcc gctacctccg cttcgacgag
     4381 atgcgccagc ggatcgacca gatggaggcc gaatccgccc gccccgactt ctgggacaat
     4441 gcccaggccg ctcaggagca catgcgcgat ctgaatcggc tgaagaacca gatcgagcca
     4501 tgggagcagc ttgcccgcga cctgagcgac gccattgaac tcaacgagat ggcccagggc
     4561 gaccctgaca tgctggccga gctggtccgt caggccgaca cgatggagca tacgctcgaa
     4621 tcgctcgaga cgcgcgccct gctcaaggat caggacgacg acaaatcggc ctatctttac
     4681 atccacgccg gggcgggcgg caccgagtcc tgcgactggg cgcagatgct cctgcgcatg
     4741 tactcccgct ggtgcgaaaa ggaagagtat gccgtcgaga cgctcgacct gatcgacggc
     4801 gaggaagccg gcatccgcag cgcgaccctg ctggtcaagg gcgagttcgc ctacgggatg
     4861 ctgaaggccg aagcgggcgt ccatcggctg gtgcggatct cgccgttcga tgccgccaag
     4921 cgccgccaca cctcgttctg ctcggtcgat gtctggccgc aggtcgatga cgaaatccag
     4981 atcgaaattc gtgaagagga tctgcgcatc gacacctacc gctcgtcggg ggccggcggc
     5041 cagcatgtca acaagacctc ctcggccgtg cggatcacgc acgaaccgac gggaatcgtc
     5101 gtcgcctgcc agaacgaacg cagccagcac cagaacaagg ccgtcgcgat gaaaatgctg
     5161 cgcgccaagc tctaccagaa ggcgcgcgcc gagcagcagg ccgaactgga cgcccgccag
     5221 gaggccaaga aggacatctc ctggggcaat cagatccgct cctacgtgtt tcagccgtat
     5281 cagatggtca aggatcaccg caccggccac gaaaccggca acgtatcggc ggtcatggac
     5341 ggcgccatcg atggcttcct cgaggcctat ctgcgctggt cggcggggat gggcgccgcg
     5401 accccgcagt cggcctgaac ccgttgaaat ccctcatccc gctcgagcag ctccccttga
     5461 gacatccccc gttcccgcgc gaaaggatcg cttgatgaaa tcacccctga gcaaatggct
     5521 gggtctggtt ctgctgctgg ccgccggcat tgccgtcggc tatctgctgg gcgcccgcac
     5581 ccccgacaac gctttcgccg gcgatgcatt ggcgcgggcc gagccggccg cctccgcgcc
     5641 ggcgaccctg ccgaccgatc cggccccctc ggccagcctg tccgatcgag cgccggtgga
     5701 ggtggccgcc gcggagccat tgtcgggcga cgaaacgccc acggcggagg cggcgacgtc
     5761 cccagcgggc gaaccggccg ccgcgcccgg cgttgagctc tccgcggcga tcaaggccca
     5821 cgccggcgaa aaaatcagcc gcggctcaag ctccaccagg aagatcgttc tgaccttcga
     5881 cgatgggccc catccgtcgc gcacccccca ggtcgtgcgg atcctcaagg aggcggggat
     5941 tcccgccaat tttttcctcg tcggcgagat ggtggaaagc tatccggagc aggccaaact
     6001 cgtcgcggac tcggggtttg aactcggcac ccactcctgg gatcatccga acctgacgcg
     6061 caagaacgag gccgaggttc gccgccagat cgtcgaaact caggacctgg tcgagcggac
     6121 caccggcatc cggccggtcc tcttccgtcc gccgggcggc aatgtcgatc agaccgtgcg
     6181 cgacatctgc cgcgaggaga atctggtcat ctgcatgtgg tcgatcgacc cgcgcgactg
     6241 ggccagcggc gccaccggcg agaaaatccg acagaaggtc gtgggcgacg cccacggcgg
     6301 cgcgatcgtc tgcatgcacg acacgaaggc gccgacgatc gacgcgctgc ccggcatgat
     6361 cgccgacctg aaggcggccg gctacgagtt cacgacgatc acgcaattgc tcgaggaaaa
     6421 ggccgccgag gacgcggcgc tggccgcaag cggcggcgtg atccccagcg gaggggatgc
     6481 ggcgccccag gcggaacccc tggtgatttc gctcgaagag agcggcagct actgatccat
     6541 caacatccca ccgagggcgg gccgggtctt gccgggcgac ggcgagcgga ccgccggcgc
     6601 ggttgcggcc cggggcggcc accccaacgg attaaagcag catgtccacc accgaccccg
     6661 tcatctggat tttctcgacc ggcacggaaa ttctgcaagg tcatgccacc gaccggaaca
     6721 gccccacgat cagcgggcgg ctgctggagg ccggcctgcc gaccgcgcgg cacgtcgcga
     6781 tcgccgatcg cgccgacgcg ctccaggaag ccctgaccga agcctccgcg cgggccgatc
     6841 tgatcatcac gaccggcggc ctcgggccca ccggcgacga tctgaaccgg cacgtcgtgg
     6901 cggcggtgtg ggggcgggcg ctggttgagg atcctgattc gctcgagcgc atccgcgcgc
     6961 gctatcagcg gcgcggtcgg gtcatgcccg agtccaaccg ggtgcaggcg ctgattcccg
     7021 caggcgccga agtcctgccg aacgaaaacg gcaccgcccc gggcttctat ctggagccgg
     7081 ggccggacgg cccgcgtgcg acgctgatcg ccctgccggg gcccccgcgc gaaatggcgc
     7141 cgatgctcga caaggaagcg ctgccgcgca tcctgcggcg tttcggtgtc gagcgcgtcc
     7201 gttttcgcgc gatcaactac cacaccgcct gcattgccga gtcggccatc aatgagctgg
     7261 ttcacgacct gttcgcctac gacgaacggg tgaatttcgc gctgctggcc aagatgagcc
     7321 tggtcgatat ccgcctgacc ctgatggcgc gcgacgaagc cgccaatgac gaactccagg
     7381 cccgatggga ggcgctggtt cgggagcgcc tgggcgagta caacatcttt ggctgcgaca
     7441 acgacacgct ggagagcgtc gttggggggc ttctgcgcga gcggcgccag acgctgacga
     7501 ccgccgaatc gtgcaccggc ggacgcctcg ccggccgcat caccgatgtc gcgggctcgt
     7561 cggaatattt ccgcgagggg ttcatcacgt acgccaacga ggccaagatg gcgcgcatgg
     7621 gcgtgatgcc cgacctgctg gcccagttcg gcgcggtcag cgccgaggtg gccgaggcga
     7681 tggcgaccgg cgcgcgcgaa gtggccggca ccgactgggc cgtcagcatc accggcatcg
     7741 ccgggcccgg cggcggctcg cccgacaaac cggtcggcct cgtttatctg gggctggcgg
     7801 gtcccgacgg gatcgtgcat agtcggaggc accattttct gggcgaccgc gccgacgtac
     7861 ggcaccaggc ggtcgtcgcc gcgcttgact tgctccggcg cggattgctc ggggcgccgc
     7921 tggatcccgt tctgccggcg caggccgtcc gtcaggaccg ctgacgttcg cggttcgcca
     7981 cgttatccgc ttgcgtttct ccggcgcgct cccgatcacg catggctgat gaatccccga
     8041 ttctccatga acgccggggt gcgaggacga cggacgaatt tctgagtgag acgaatctga
     8101 gtaagggaac ggatccggtc atggctcaca tcaccatccc cgaagccgag gcattgctcg
     8161 atcaggaaat caaggtcctg gacaaggggt tcgtgcggct cgtggattac ctggggggcg
     8221 acgcccggat cgttcaggcc gcgcgcgttt cctacggcgc cggcaccaag accgtccgcg
     8281 aggatcgcgc gctgattcat tacctcatgc gccaccgcca cacctcgccg ttcgaacagg
     8341 tgatcctgac atttcacgtc aagctgccga tcttcgtcgc ccgccagtgg atccggcacc
     8401 gcacggcgcg gttgaacgaa atgtcgggtc gctattcggt gatgaaggac gagttctatc
     8461 tgccggatcc cgagcagatt cgcccccaga gcaagtccaa taaacagggg cgcgacgccg
     8521 acgcgcttga cgccgagcag gcgcgccgcg tcctcgaaat cctcacctcg gcgcaggaga
     8581 ccgcctatgc cggctaccgg gagatgatcg acctggatat cgcgcgcgaa ctggcgcgcg
     8641 tcaatcttcc gctgtcgctc tacaccgaat ggtactggca gatcgacctg cacaacttgt
     8701 ttcatttcat ccagttgcgg gccgacgagc acgcccagta tgaaattcgc gtctacgccg
     8761 aggtgctggg cgattgcgcc cgcgccgtcg cgccgctggc ctatgaggcc ttcgaggaat
     8821 acgttcgcca cgcgcggcag ttttcgcgcg aggaaatgga agccctgcgc gcgatgctgg
     8881 ccggtcagcc ctgtccgatc gaacaggggc ggcggcgcgt cgagtttttc cagaagctcg
     8941 gactggacga tccggccctg caaccccagg aagcctgagc tttatcctta tcattccgtc
     9001 aatttcagtt aattcattgg aattcacacc atgacgactt tcgccccgcc gcgcggcatg
     9061 cgcgatttct atcccgagga ttttcgcgcg cgcgacgccc ttttctccgc ctggcgcgaa
     9121 accgcgcggc tccacggctt tgaggaatac gacgcgcccg tcgtcgaaac catggaactg
     9181 ctcgagcgca aggccggcga ggaaatcagc gaccagatct acaccttcgt ggacaagagc
     9241 gatcgccgcc tcgcgcttcg gcccgaactg accccctcgc tcgcccgcat gatcatcgcg
     9301 cggcaggggg cgctcagctt cccgctcaag tggtccgcca tcggccagtg tttccgctac
     9361 gaacgaatga cgcgcggccg caagcgcgag cattaccaat ggaatctcga catcgtcggc
     9421 gaggaatcgg tgctggccga ggccgaagtc attggcgccg ccgtcgccgc cctgcgtcgg
     9481 ctgggtttga gcgccaacga gttccaggtc cgcgtcggca gccgcgccgt gctggccgag
     9541 ctgtttgccc gcagcggcat cggccaggag cacttcatgg cctgttgcct cgcgctcgac
     9601 aagcgcggca agatcagcga cgacgaggtg gcccggctgc tggaggagga gggcctcgcg
     9661 acccgtgata tcgaacgggt gttcgaactg ctggcgatca cgacgctgga tcaggcggcc
     9721 gcggccgtcg gcgccgacag cgccgcgctg gcccagatcc gcgaactgtt ttccctcgcc
     9781 ggcccgctcg gcttcgcgcc ctttctcacg ttcgatctgg ccgtcattcg cggactcggc
     9841 tactacaccg gcatcgtctt tgaagcgttt gatacggccg gaaagctccg cgcgatcttt
     9901 ggcggcggcc gttacgacag cttgctcagc aacctcggcg gtcagaaaat gccgtgcgtc
     9961 gggcttggct tcggcgatgt ggtcgtgatg gaactgctcg ccgattgcgg caagcttccc
    10021 gcgaccgccc gccgcgtcga tttcgccatc ggcttcatgg acgacccctc gcgcgacctg
    10081 gccctgcggg ccgccctcgc cgtgcgcgcg gcgggcaaga gctgcgatct ggcgctcgcc
    10141 gccgaaaaac cgaagaagtt cttcgcccgc gccgaccgcg ccggcgccct ccacgcgata
    10201 tacatcggcc ccgatgaagt ccagtccggc acgttctcga tcaagccgat ggatgaccgc
    10261 aggccatgga ccgcttcgct tgactcattc gcggagcggc tggccgggcc agcctgaccg
    10321 gccccggatc gaccggaatc ggccggggcg atcggctcca gattggtgtt gccgcgccgc
    10381 gctgcaccgt cccgccatcg cgatccttcc atccgccggc gcgcgcgcga cctgccccag
    10441 accattgcca atcgctttgg cactgtctat tctgtccctg acgtatgtca tccggagccg
    10501 gttgtcgggc cttcgccaag ccggcctggt ccgatccccg ctgggagaac gccatggcgc
    10561 gtgtcatgat catcggctac ggccccctgc cccgcacggg catgccctac atcggggcgg
    10621 cggccctgcg cacgcgccat ctgctcaaac cgattctcga tgccggccat acggtcaatc
    10681 tctataccct gccgattccc gccaccgagg gcaccgaggg cgaagtctcc tcgatggttc
    10741 ccgacaccta cgaagggctc acctaccagc gtttcaccaa ccacagcgct gaattcgccg
    10801 tccgcatgct gaccgagcag gtccgccagc tcgaacccga cgcactggtc ggcgtgaaca
    10861 cgtatccggc ctacatcgcc tgcctcctgc cgaccgcgct gccggtgtgg gccgatctca
    10921 acggctactg gatggccgag atgcagggcc gctgctggat cgaggaggat gactcgatgc
    10981 tggccgacgc gtgggcgatc gagcgggcca tcgtccgccg ctgcgacaag ttcagcgctg
    11041 tttcccgtcc ccagctgcac gcggtcctgg gcgagatggc ggcggtcggc cggctcaatc
    11101 gccatacgtt cgattatcag ttcggccatc acctgcccaa tgcctgctac cgctggcagg
    11161 tgacggcggg cgaggggagc gccgccggcg agccgctgct gcgcgggccg gtcgtccccg
    11221 aggacgcgtt cgtcctcctc tggagcggcg gattcaatgt ctgggccgac gtcgccacgc
    11281 tggtgcgggc ctgcgacctg ctgatgcaga atcatcccaa cgtccacttc gtctcgaccg
    11341 gcggacgcat cgagggcgtc gtcacgcgcg tctacgacga tttcgtcgcg ctggtcgagc
    11401 agtcgcccta caaggaccgc tatcatctgc tcggctggat cgagtcggac aagctgccgg
    11461 cgattcaccg cgaggccgac gtcgggctga acgttgattc gcgcaactac gaaacgatgt
    11521 tcggcgcgcg caaccgcatc aatgccatgg cgatcgaagg ccttgccgtc gccaccacca
    11581 tcggcaccga aatcagcgaa tggctcgacg atggccacgc cgtcatcgcc gccccgatgg
    11641 gcgaccccga agcgctcgcc tacagcatcg cgccgtggat catggatcgc agtcagctca
    11701 aggtttttgc gcagaaagcc aagcggatca tggaggagga tttcagctac gagcgcacca
    11761 cgcgcgggct ggtgcagtgg ctggaagcgc cggcactggc gcccgacaat caggccaaga
    11821 tcagcagccg cggcggccgc gtcgccgatc tcaataccgt ggccgtgaat tcactggagg
    11881 aagaagccct gctgctggcg cgccaccgcc tgccggagat ccaggatgcc ctggcccagg
    11941 cccgcgcccg ggaggaaagc ccccgcaaac gcttccgatt ctggtaggat tcgtccccct
    12001 gctaatttcg tccctcactc gaaaatttgc ccgccattct cggcgtcgcc tggatcggtt
    12061 ctgccgtccc tgcgggactc ggtcgcgatg gggatcccgg ttcccgccac ggacgtggcg
    12121 ggctgaagtc tgccgtccct gacgggactg ccctgcgtga ggatccggca aggctcggag
    12181 cgttcgaatc ggttgttccc tcccaatttt catccctcgt tgtgcaccgc cggttcatga
    12241 gcagctcgca attgtccctc gagtcatttc tccccaaccg ctctcaacac caggcgcgtc
    12301 agcatttcaa gatgctccgg gcgcgtattc agtgccggaa ggtagcggta gtctctcccg
    12361 ccggcatgga gaaatcgttc cctgttcgtg atcgcgattt cctccagcgt ctccaggcag
    12421 tcggcggcga aacccgggca gagtgtatcg acccgctcga cgcccgcgcg cgcccacgcc
    12481 atcagggttt catcggtgta gggccgcagc cagggttcgc ggccgaaccg ggactggaag
    12541 gccagatacc gattgtcggc cggcagttcc aaggcatccc acagcaactc cgccgttcgc
    12601 cggcattgtt gcggataggg atcgcccccc gcggcctgac gctcgggaat cccatgaaac
    12661 gacaacagga gccggtcggg ccggcccagt tcgctccagt actggcggac gccggcggcg
    12721 agtgccgcaa tgtaggccgg ctcgtcgtga tagtccccga tgaaatgcag ttccggcacg
    12781 cggcgccagc gaacgatttc cgccgcgacg gcgtcgaatg tcgaccccgt cgtcgccccg
    12841 ctgtattgcg gatagagcgg caggacgacg agccgccgcg gcgcgcgccc gcgcagcgca
    12901 tccagcccgc gcgcgatcga tggctcgcca tagcgcatgc cgatctccac ggcgacctcg
    12961 cgacccaggc gcgcccgcag ccgcgcggcg aggccgtcgg cgatcgcccg cgtcgtcacc
    13021 aatagcggcg acccgtcggc cgtccagatg ttccggtaga gccgcgccga ccgggccggt
    13081 cgcctgcgca ggactatccc gtgcaggacg ggccaccatt tcagccgggg cagttccacg
    13141 acccgtggat cgctcagaaa ctcggccagg aacgggcgca gcgccgcggc cgtcggcgcc
    13201 gccggcgtgc ccagattggc gatcagcacg cccaggccgt cgaccggttc cggggcgggg
    13261 gcggctttga attgcgccat cgcggcccct cctctcaagg gcgtctcgcg tcgctcatga
    13321 tcgaccccct ctggaaccac ggctcatgcg agcgcttgcg ccggcgctta ctccgcatgg
    13381 aaccacgcat gcagttcatc cagaaaatca taggccgtca gatcgaactg gagcggggta
    13441 atcgagacgt aatcctgata cagggcgaca tcatcattgc cgtcgtcggg gtgcgtgctc
    13501 ggattccagc ccttgccaat gttgcggtag atgatgcgct ccgaatccgc ttcctcgggg
    13561 caggtgaaca ggtcttcata gccggaattg cccatccgcg tcacggccca gcccttgatg
    13621 gcgtcgtagg gcaccggcgg cacattgacg ttgagcatca cgccgggggg cagtccgcgc
    13681 tccagcacgc gccgggcgac ctcgaggccg acccgggccg atgtctcgaa cgtcagcttg
    13741 tcgggatcgt gatagagctg ggagacggcg atggcgggaa accccaggat ggcggcttcg
    13801 cgcgccgccg ccaccgttcc cgagtagagc acattgaccc ccgcgttctg accgtggttg
    13861 attcccgaga cgaccaggtc gaccggccgg tttttgcaca ggagcgtcag cgcgatcttc
    13921 acgcagtcgg ccggcgtccc cgccagaccg taaccccaca gcttgccgtc gcggtgatgc
    13981 ggttcgaaga gcatgtcctt gaagaccgtg atggcatgac ccatgccgct gcgctcgata
    14041 tcgggcgcga cgatcgtcac gtcgccgatc tggttcagcg catcggccat cgcggccaga
    14101 ccgggcgcct cgattccgtc gtcgttggag agcacgatgt gcatcgggtc cgcctctcag
    14161 gcctcgtcgg tcgcttccag cccctggcgg gcgcggcgcg cttcgtccca cagcgttgcc
    14221 acgcgccgcg gatgaccggc cgcctcacgc agtgcggcgc gccactcgtg cagcacgtct
    14281 tccatctgat ccaggcaggc gaggacctcc tccgcatttt ccgtcagaat ctggcgccag
    14341 atctccgtgt cgccgccggc gacgcgcgtg gtgtcgcgca aaccctggcc gcatagcccg
    14401 cccagttccg cggccggcgc gagatggcgc gtcagcgtct ggatcagcgc cgccgcaacg
    14461 acgtgcggca gatggctgat gccggccagc agccggtcat gccgcgccgg atccagctcc
    14521 acgacgcgcg aaccaacggc ttcccacaac gcgcgggcga gggcgagcgc ttcgggcgcg
    14581 gtgcggccgg tcggcgtcag cagacaggga tacccgacgt agaaatccgc ccgcgcatgg
    14641 tgggccccgg ttttttccga tccggccatc gggtgcgagc cgacgaagcg cgcgtgggac
    14701 gggagattgg cctccgcggc ttcgacgatc cgggccttga cgctccccac gtcggtgacg
    14761 aacgtccccc gctccagata cggcgcgatc tgcgcgaaac tttcccggat ctgaaccggc
    14821 gggatggcca gcacgaccgc atcggctccc gccagtgccg ccgccggatc ggtctggaac
    14881 tcgtcgagcg ctcccaggtc gcgtgcgcgc gcgagtttct ctgccgagcg cccgagcccc
    14941 acgacgcggt gcgccagccg ctggcgccgg acggccagac ccagcgaccc gccgagcaag
    15001 ccaaccccgt ggatgaccag acgctggaaa ggcagagcac tcagagcagg gaagatccgt
    15061 cgagagtcga tcggcgggtg atgaccaggc cgaaatcgga gaggcaaaac ggccctcagc
    15121 ttatcgagac ccaccagggt gcgcaacttt ttcggcggat tggaaagcgt ggacgctgcg
    15181 gacggagtgg acagcctgaa cctggccggt ccggcggcgg tcgattgaat caacgacggt
    15241 gttatatcgg gttcacataa gactgtccga tgagcctcgc ggcgcgatac catgggcggc
    15301 atgaaccagc gggcggctcc cgggggaagg cttcgggagc gatcaggaac gcgaggacga
    15361 gggacgaggg acgatttcag aatcggggac gaagatcccg gggaggacga gacctttgtc
    15421 cgaactcagg cagaacctgg ccacccgcga atgggtgatc atcgcgacgg agcgggcgcg
    15481 ccggccgcat gaattcaccg accccgcgcg ccagttgacg cacgaacgcc ccgcccacct
    15541 ggccgcctgc ccgttctgtc ccggccagga gtatgagacg ccggcgccgg tcctgcgctg
    15601 gccggcggag ggcccatggc ggctccgcgt ctttcccaac aaatatgccg ccctcagcgt
    15661 gaacggcgag aaaagctacg ccctggaagg ggtccggcgc aaactgaccg gcgtcggcta
    15721 tcacgaggtg ctggtcgaat ccccgctgca caacatgagc cctgcctgcc agagccccag
    15781 ggcgctgacg cagacgctcc gggcgttcca gacgcgggga cgcgtcctga gcgaggaccc
    15841 gcgcatcgaa cagatcttct acttcaagaa tcacgggccc ggcgcgggga cttcgctgga
    15901 gcatccccat tgccagttgc tcgcgctgcc gatggtgccc catcacgtgc ggatgcgcat
    15961 cgaaagccag cgcgcctatg tcgacgacaa cgccgtctgc ccctattgcg tcatgatgca
    16021 gcgcgagctg atcgacggcg aacggatcgt gggccggaac cgttacttcg tcgcgctggt
    16081 cccgttcgcg gccacctcgc cgttcgaaat ctggatcatc ccccgccgcc atggccccac
    16141 cttcctggat ctgagtctca acgagctgga gcacctggcc gagttgatgc gcctgctctt
    16201 cggcaagctc taccatggcc tgggcgaccc tgattacaac tacatcatcc acagcgcccc
    16261 gctgcgcgat tccgccagcc gctacctgca ttggtatatg tcgatcgttc cgcgcgtgac
    16321 gaaggccgcc ggctttgaac ttggcagcgg gatgtatatc aatcccgcgc tccccgagga
    16381 gagcgccgaa ttcttgcgca atcaggatcc cggccagccc ctcgaggacg aagaggacga
    16441 accggtcgat cagttgcgcc agagctcata atgacggttg cgccacggcg tcggctgacg
    16501 cagcagccgg cggctgaggc tgcgcagcgc gatcgcaatc gtgaacgcgg cgctgagcgg
    16561 cgtcagcagc agcgttcccc actttccctc ccccgcatgc acttccacca gggcgcggtg
    16621 cagccccacg gccgcgatct ggatcgccgg caggatcgtc ccgctccagg gcgacagccc
    16681 cagccacggc gacagaatga aacagaccgg cggcagaatg aacacccccg tcaggcaggc
    16741 gatcgcgagc agcgtctgat accccgggaa cactgcatgg aagtttttcg aaaatccctc
    16801 ccagacttcg gcccgtgagc ggtacattcg gcagcggacg tgacgggtca cgtcggtcag
    16861 atgcgtccga aagccggcgc gcttgaccgc gcgcgccagg ttcaggtcat cgacgatttc
    16921 gccgcgcacc gcctcgtgcc cgcccacggc gtcgtaggcc gcgcgccgga tcaggatgaa
    16981 cgcgccgctg gccgccgccg cgcgccggtg attcagccgt tccatccagc ggaacgggat
    17041 gccccccagc gccatcgtct gcagcagcgg caccgacagc cgttcccaga tcgtcaccgc
    17101 gacgagccgc ggcagcccgc tgagcagatc cgaatggcgc gcccgcgcgc gccgcatcgc
    17161 cattgtcagc gttccccgcg cgaactccgt atcggcatcg gtaaacagca gccagtcgcc
    17221 gcgggcggcg gcggccagtt gccggcacgc gtgcggcttg ccgatccagc cagactccag
    17281 aggacgacct tgcaataccc gcaggtgggg atatcgggcg gcaatcgcca gcgcgatctc
    17341 cgccgtgcgg tcctcggagc agtcgtcgag cagcagcacc tcgtagcagg ggtaggcctg
    17401 ccgcgcgaga ctttccagca gtggcccgac gttgtgttcc tcattgcggg cggggatgca
    17461 gactgagatg aatggggcat cctccggcaa aggcggcagc cgcgcgtcgc gtcgccgttc
    17521 ggcccgctga tcgaccacgt tcagccagcc cgacagcacg agccacgcac acagaccgac
    17581 aatgactagc tgcacgagca gcaacatggc gcgccccgat cacgcattgc agccggcgcc
    17641 ccgcggagga agacgggccc gctcagctta cccgccggtg gcagcattgc caagccgccg
    17701 atccgccccg tatactgcgg ggcattccac ggagcggtga aacttcccca tgccgcatca
    17761 gatgaccgcg ctgcgccgac ggagctggtt gatcccggca acggttctgc tcgcgctggc
    17821 gttcggcgcc atcctggccc cgtggatcgc cccgcacaac atccgccgcc tcgagacgct
    17881 gcgtctggcc gattcaggac tgccgccggc ctggctcgcg gcgcctccgg ccggcgagcc
    17941 cgcgccgcgc acgccgttct ggctgggcac cgatgcccag gggcgcgacg tcctctcggc
    18001 gatcctcttc ggcctgcgcg tctccctgct cgtcggcatg gtcggcacgc tgctcgcgct
    18061 gaccgcgggg acgctgctcg ggctggcggc gggctattgg cgcggctggg tcgacggcgc
    18121 cattatgcgc ctggccgacg tccagctatc cttcccgtcg atcctgatcg ccctgttcct
    18181 gatggccgtc tggggccagg ggctgctcaa gatcatcctg gccgtcgcga tcgcccactg
    18241 ggtgatctac gcgcgcgtgg tgcgcggcgc cgtgctggcc gagcgcgaga aggactacgt
    18301 cacggcgatc cgcgcgctgg gcgccggcgc gccgcgcatc gtgctgcgcc acatcctgcc
    18361 caatctggcc gcgccgatcc tggtcatctc cgccgtcgag ttcgcctcgg tcgtgatgct
    18421 ggaggcaacg ctcagcttcc tcggcctcgg cgtcccgatc acccgcccct cgctggggat
    18481 gctgatcaat tttgggtttc aggacgtctt cggcggcacc tggtggacct gggtttttcc
    18541 aggcctggcg ctggtcctgc tcgttctgtg cctgaactgg ctggccgaca tcctgcgcgt
    18601 ccgcctgatg cccgaaggcg aggcgtaggc gcaaaacgac gccgccgggg gctccgtgcg
    18661 agcaccccgg cggcgatcat tcctcgggaa agcgggtcag tctgtaaaac gcgcaggccc
    18721 ccaaaccggc attgctaagt agttagaagt gcggttggcc tgggagcccg tcgtcctaca
    18781 tgcccaagcg gacgacgttg gtagccttat cccctttggg cccttcattc aattcgaact
    18841 ggacgaactc ccctttgacc agggtgcggt agccatcgcc gacgatgtcg gagtagtgga
    18901 cgaagacatc ggacttcgcc tcgcattcga tgaaacccca ccctttcttg tcgtggaaaa
    18961 acttcacccg accagtggcc atacagcagt acacctccct cgaataaaga cgcttacgcg
    19021 acgacatcaa agcggacggt ggatagaacc gtgcgcttca gccggtccgc tgaatccttc
    19081 cggcagcgtt acacggaccg gtaacaacct ggtgaaccgg cacgcacagc cccgaggtca
    19141 ttcgccgccg ggccgcttcg ccaagttttt cactaacgct gtctgaggat tctgaaccca
    19201 ggaccggttt gagtcaagtg catttcttga cggccagatc cctttggccc tccgctcttc
    19261 tcgaattatc gtctctcgtc ctcgtctcgt gaccgccccc gttcctcgtc ctcgacgctc
    19321 gaaatccgtc gtattttgcc ttccttcctg attttccgga aagccgcggg gccctttcgg
    19381 actttcccat tgactttcta tctcaccaaa gtaagctgca agagatagtg tagtctggaa
    19441 cggacacctc attggagtgc ccgcttccgg acgacgggga cgccctcccg gccccctgcg
    19501 ctatcgcccc tgatccggcg atcccgggag cttccgctcc cggttccccc ctcgattcag
    19561 gatatctcgc acccggcgga tgtttgaaag gagattgacg attatggcgg ttaagatcgg
    19621 catcaatggc tttggacgca ttggccgact ggtgttccga gcagccttgg aatccgatgg
    19681 cgtcgaagtc gtcggcatca acgacctggc ccccaccaag agcctggccc atctgctcaa
    19741 atacgactcc aaccacggca cgctgcgcca ggaagtcggc ttcgatgacc agcacatcat
    19801 cgtcgccggc aagaagatcc ccgtgttcca gcagaaagat cccgccgccc tcccctgggg
    19861 caaggtcggc gcgcagatcg tgattgaatc gaccggcctg ttcaccaagc gcgccgatgc
    19921 ggccaaacac atcgacgccg gcgcgaaaaa ggtgatcatt tccgccccct cgggcgacgc
    19981 cgacgcgatg ttcgtcatgg gcgtcaatga caagacctac gacccggcca agcacaccgt
    20041 ggtttccaac gcctcctgca cgaccaactg tctggccccg atggccaagg tcctgcacga
    20101 ccacttcggc gtggtcaatg gcctgatgac cacgatccac tcctacacga acgaccagcc
    20161 gagccaggac cagatccaca aggatttccg ccggatgcgc gccgccgccg tcagcatgat
    20221 cccgaccaag acgggcgccg cctcggccat cggcaaggtc atccctgatc tgaacggcaa
    20281 gctcgacggc tacgcgatgc gcgttcccac gcccaacgtc tcggtcaccg acctgacgct
    20341 caacctgtcc aagccggcga cggcggcgga gatcaacgcc gcgctgaagc aggccgccga
    20401 gggcgaactg aagggcatcc tcggcttcga ggaagacgag cttgtctcga tcgacttcaa
    20461 ccactgcccg ctcagctcga tcatcgatgg tccgtcgact cgcgtcatcg acggcacgat
    20521 ggccaaggtg ctgagctggt acgacaacga atggggctac tcgctgcgct gcatcgatct
    20581 ggccaagaag atcggcgcat cgctgtaaga ccgccacggc ggttcaagcc tgatgcgggg
    20641 aagtcggttc gacccccgac ttccccgcgt tttcccttcg taattcgacg cccggctctc
    20701 ctgctcccgt tcaccgaccc accggccggg catgtgctct gtcgcttcgt tgaaaccccg
    20761 actggaagga tttgtccatg cgccgtccga tcgtcgccgg aaactggaaa atgaacaaga
    20821 caccgagcca ggcgcgggcg ttgcttgagg aattgaagcc gctcgtcgcc gacgccccct
    20881 gcgacgtcgt cgtcgcgccg ccctttgtct gcctggagcg ggccgcccag acgctcgccg
    20941 gctccgccat cggcctggcg gctcagaacg tccactggga acccagcggc gccttcaccg
    21001 gcgaggtctc gatcgaaatg cttcaggatc tgggcgtgcg gttcgtcatc atcggccaca
    21061 gcgaacgccg ccagtatttc ggcgagaccg atgagacggt caaccggcgc gccaaggccg
    21121 tcctggccgc cggcctgacg ccgatcatct gcgtcggcga gcaactccag gaacgcgaga
    21181 gcggccagac aggtgcggtc gtcagccgcc aggtgcgcgg cgccctcgcc gagctctccg
    21241 ccgcgcaggt cgccggctcg atcatcgcct acgagccggt ctgggcgatc ggcacgggca
    21301 aggtcgcctc gaccgagcag gcccaggaag tccatgcgct gattcgcaag ctgctcggcg
    21361 agctctacgg cgccgccacc gccgaccagg tgcggatcca gtatggcggc agcatgaaac
    21421 ccgacaacgc cgccggcctg ctggctcaac ccgatatcga cggcggcctg atcggcggcg
    21481 cgtcgctcaa agccgccgac ttcgccgcga tctgcaacgc ggcgcggtag catcggccca
    21541 atcctttgcg gccaatcgtt gattgcggct ccgccgcgtc agggtttcat ccccctggtc
    21601 ccgcggtttc gcatcacccg ccggtcggcc gccggcccga cgcgatctcc gcttccacgc
    21661 gcttcaggtc cgcatccacc atcatctcga ccagttcctt gaaccgcacg cgcggctgcc
    21721 agttcaggtc gcgctgggcc aggctgttgt cgccgcgcag cgtgtaaagt tcggccggcc
    21781 gcttgaacgc cggatcaatg aacacatagg gctcccaatc ctcgatgccg acgcggcgga
    21841 acgccacttc caccagttcg cggatcgtgt gcgtctcgcc ggtggcgacg atgtagttct
    21901 tcggttcggg ctgctggagc atgcgccaca tcgcctcgac gaagtcgccc gcgaagcccc
    21961 agtcgcgctg gctgtccaga ttgcccaggt gcagccgctc gttcagcccg agcttgatcc
    22021 gcgccacgcc gtcggtcacc ttgcgcgaaa cgaactcctt gccgcggatc ggtgattcgt
    22081 gattgaacag gatcccgttg acgcaataca tcgcgtagct ttcgcggtaa ttgaccacga
    22141 tccaataggc gtagagcttg gcgatcgcgt acgggctgcg cggatggaac ggcgtatcct
    22201 cggtctgcag cc
//
LOCUS       DAOPCO010000037        21922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2527_length_21922_cov_4.525312, whole genome shotgun sequence.
ACCESSION   DAOPCO010000037 DAOPCO010000000
VERSION     DAOPCO010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_21922_cov_4.525312"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PLS90_04695"
     CDS             <1..818
                     /locus_tag="PLS90_04695"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK01736.1"
                     /translation="AAIRRHGAALRAIRIGRVADELTAILTGPRPGRAMHLMDDLGLW
                     THVIPEIEPMKGCPQPPEFHPEGDVFVHTALVLDLLHEAWGGAPPPELAWAALLHDVG
                     KPPTLTHDGRIRFPEHQRVGAAMADQILRRLRLPNRIREIAVQLVAGHMRFMDVADMK
                     QSKLRRFLGQPHFDQHLALHLADCRGSHGKLDNYRFCLDQAERFRRDDAQAALLPEPL
                     LTGHDLIALGLKPGPAFSRILEAVRDEQLEGHLADRDAALAWLRDHRADFETI"
     gene            917..2410
                     /gene="rfaE1"
                     /locus_tag="PLS90_04700"
     CDS             917..2410
                     /gene="rfaE1"
                     /locus_tag="PLS90_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPK01737.1"
                     /translation="MGRKPTSPPEPAPDHLRAIVERFAGRRVLVVGDVILDRYLWGDV
                     ERTSFEAPVPVVNLRAETDRPGGAANVVANLAALGARPQLVGVVGADGEGRAVRDLLR
                     AMRVSPQGLVVDRARPTIVKTRVIAQRQQLMRLDREETAPISPAVARALVARARKALA
                     GAAVMVLSDYAKGTLGPDVCRELIAAAHNAGALVVVDPKGTDFSKYRGADIVKPNRRE
                     AEAASGRPIDSPAALDAAAAALRRAVRGRALVITLDGGGAAIFESGRPTVRTAAQALA
                     VFDVTGAGDSFIAAMSAALAAGAGIADAARLGNLAGSIAVGKLGAATVEPAELLRALD
                     PGAGHRKLRSCAQLLADLAPHRALGRRIVFTNGCFDLIHLGHLKFLEQARRLGDLLVV
                     AINSDASVRAVKGAPRPVLGQNERAAILGALEAVDFVVIFDERTPERLLEQIRPDILV
                     KGKALRAGQIVGADLVPAWGGRVVSLPLLGEMTTDRLLERIARQSRP"
     gene            complement(2846..4837)
                     /locus_tag="PLS90_04705"
     CDS             complement(2846..4837)
                     /locus_tag="PLS90_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01738.1"
                     /translation="MVRPVPYRLATLRRLTLGVCVAWGLLAGVVGAAEIQVGSRIETT
                     ARVNVRPRPSMAARPIAVRKPGSAGTVMGGPVRAENYTWWRILYDNGIHGWSVDVKLR
                     VIAAAAPTPEPTPEPTPEPTPRAEPTPEPTATPTPEPRPTLAPTPEPTPRATPKPAVT
                     PTAKPEPTAEPTATPTPEPTAIPTPAVELPGAIATPQPLPVETPDADTGAEPAPAATP
                     TAAAAAVNEGAPAAAREPAGSRYLVERLENVPLERTSKLVFGQPAAAPPPKGAPFIQP
                     QTGHRITRLTEIGELRPERVRDVGRGPFAESPDRGFYNGYARWTNVNVDGRYAIAFRT
                     DNQSYLVRLSDLALLGPVESAPGRPLGETHEVRWDLSGRPGTETTIYYHVGPLFYRQD
                     AVQGPASAEVLHDFITPIVPTAEMAPSDDARYWAFRLADGRCVVYDKDRDKVLPGWIE
                     QKPNALDISPSGRWFVVLEHTNGDPALGPRFYRIADLAQGDTSRPVYLPTRSMGHNGW
                     ALDKQGREVLVFQDNTNDWFSAFEPESGDRIDIFNMAEAGYPLHQHIGRLRAPEKRGW
                     FLMSTYTPFDDRWAANQLLMIEIEHYALHPRVWRLGSTLNHRWRGSEDVGGYFAEAFA
                     SIDHAGNNIYWGANWMAQDNLELYRMELPPDWHEALGGE"
     gene            complement(4919..5494)
                     /gene="hpt"
                     /locus_tag="PLS90_04710"
     CDS             complement(4919..5494)
                     /gene="hpt"
                     /locus_tag="PLS90_04710"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPK01739.1"
                     /translation="MNQPQKVTANPVDLLQDLERVLISEGELADRIERLGAEISRDYA
                     GRDLVLISVLKGGIVFLADLMRRITIPHAIELVGASSYKGGTVALGGVRVTKDVDQSI
                     AGKDVLLVEDIYDTGNTLHVIHDLLRLHNPASLEICALLRKTKPQVQKLGVRYLGFDI
                     DDVFVVGYGLDFKERYRNLPCIGVLKHALYE"
     gene            complement(5796..6377)
                     /locus_tag="PLS90_04715"
     CDS             complement(5796..6377)
                     /locus_tag="PLS90_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01740.1"
                     /translation="MESLFHPRLVLALALLLGGLAAGGCSRNKYQIEIDRRFSMNLVR
                     VPDWYPPANQPLGPAEADVLQRMGKPDFFRLWWREDGSLITSSDLSGRIDQVPETLQV
                     MQRSWLYPALEQEVLFLNDGQSYRVQPMSEVIKLVARYGDPSSRTPPVLRNGRMYETW
                     QWIEYGLQIEMVDGLPGKVTHFPATGTGTYLGK"
     gene            6955..8556
                     /gene="nifA"
                     /locus_tag="PLS90_04720"
     CDS             6955..8556
                     /gene="nifA"
                     /locus_tag="PLS90_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HPK01741.1"
                     /translation="MKETADGTPPSGGATPLGSVRREVRELSLLFEISQILDSAIDLR
                     EVVQQVLIAISNHMGMSRGTLALLNRDTREVMIETAHGLSNSQKERGRYKLGEGITGK
                     VIETGRPMIVPDIDAEPEFLNRTGARRRRDRKTIGFLCVPIKQGQEVYGALSVDRFSE
                     GGVSLDEDLRLLQIISSMIAQAVRLRQSLREERERLLAENQRLQEELQDRFRPANIIG
                     HSNVMQNVYDLIAQVSKSNTTVLIRGESGTGKELVAHAIHYNSLRANKPFIKVNCAAL
                     PESVLESELFGHEKGAFTGAISMRKGRFELADGGTIFLDEVGDFTPATQIKLLRVLQE
                     REIERVGGSRTIPIDVRVIAATNRNLEQMIETAEFRQDLYYRLNVFPVHMPPLRERKS
                     DVLELANHFIEKYSRANHKNIRRISTPAIDMLMAYHWPGNVRELENCIERAVLLSTDS
                     VIHGHHLPPTLQTAEASGTTFTGSLPETLDNFERELILDALKSARGSMAKAARALGLT
                     ERVMGLRVKKHGIDPRRFRMPRSFA"
     gene            complement(8585..9010)
                     /locus_tag="PLS90_04725"
     CDS             complement(8585..9010)
                     /locus_tag="PLS90_04725"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPK01742.1"
                     /translation="MTEQGATIRKRGLTLVIEKESVQLEEIELHRLSTVLLYGNIQVT
                     TQALHALQKERIELALLSQAGRLPGRLGVATLRIIFELIVSLVMNQLLKFLGAVLLCS
                     GRGFAPFGDGIVWMASSKSRCAPAWAIRMAPLRGGRARG"
     assembly_gap    9138..9237
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9332..10051)
                     /locus_tag="PLS90_04730"
     CDS             complement(9332..10051)
                     /locus_tag="PLS90_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01743.1"
                     /translation="MKWYPLAGLACLAVLLLGCSHALEVRNLRSYESMQIQPLHNPPI
                     TVGIVPSTTDIESEKLIKGIGTALERHGATVIMPYAVGSALKPDVIANIAVRPEYRGS
                     GWNFLINFPGFLVWAPAWHGYHYNVDYDMQVSLTDAARNTQIDSWAVPVELNVRHADM
                     DRTWTEISWLEWGVIALVGGFVFINYDEDVTPLLVDHLETPIGDFIAQQIIARLNGSG
                     IGREAASLPPDNREPPVISIH"
     gene            complement(10098..10568)
                     /locus_tag="PLS90_04735"
     CDS             complement(10098..10568)
                     /locus_tag="PLS90_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01744.1"
                     /translation="MALIQCRACGHGVSRNAAACPNCGEPIKKSPGSYGSWGCLLIII
                     VMMGACFHSVTTRSAEENLRHINNPDAWRSDDASSMAWTMMRGFVRDQLKAPKTADFP
                     LMPLSLERDGQKHRIRGSVDSDNSFGAMIRTHFRGEVEPTSREQSRLNEPGSPA"
     gene            complement(10573..10743)
                     /locus_tag="PLS90_04740"
     CDS             complement(10573..10743)
                     /locus_tag="PLS90_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01745.1"
                     /translation="MDLPLERVQAIERESRETIPRLHGNDLQLRLILRMAAQPLEEAA
                     KVAKKRQEAKKG"
     gene            11002..11089
                     /locus_tag="PLS90_04745"
     tRNA            11002..11089
                     /locus_tag="PLS90_04745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11036..11038,aa:Ser,seq:tga)
     gene            complement(11264..12658)
                     /locus_tag="PLS90_04750"
     CDS             complement(11264..12658)
                     /locus_tag="PLS90_04750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01746.1"
                     /translation="MIGLIGRLREELVSLYERTRVRSWPQGSPLRIQLYDGAGDRRQL
                     AAALDAVGTFHPGSDVTIFTEEAAGGAALDAQRDARAIARLEVRALDDAGARGAADAR
                     ILLSPQPEATAAGLGRLRPARRAFMRSALRDRRARAWFLYRDLKLWDARPGACLLRRV
                     LLPVLSPLNRWADRLERERIARRVRPHLTGAPGLPDEGPCRHARRFRVFSVPRPIDFC
                     PDCQMGLTPPEHLETGDSLAAFYDAAYPRHGLYQGDGVFAEHVRENGERVEAYLAALR
                     PAGAWNDAGEGAAGDGGGPKVLDYGSGNGRSAIFWLERGWRYVGVDPSAENVAFARGL
                     VRRMAGAGEERAALGCGFAEHPLIAASAPFDLIFLCHVLEHVPDPVGLLAELRRHAAA
                     GGGLYVEVPDGRRYTWDPRHRGYANKEHLWDFTAAALERLVEAAGWKAPATHVDPDEN
                     RPFLALMARNGGRR"
     gene            complement(12985..13452)
                     /locus_tag="PLS90_04755"
     CDS             complement(12985..13452)
                     /locus_tag="PLS90_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK01747.1"
                     /translation="MAVKPVPENFHTVTPYLTCGDGVKALEFYKAAFGAREMYKMLTP
                     DGRLGHAELRIGDSAVMLGGECLEYGNKSPQTLGGTPVNLYVYVEDVDTAFERAVAAG
                     AKVRMPVMNMFYGDRTGQLEDPFGHVWSLATHVEDVAPEELDRRAREMFAQKP"
     gene            complement(13715..14299)
                     /locus_tag="PLS90_04760"
     CDS             complement(13715..14299)
                     /locus_tag="PLS90_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HPK01748.1"
                     /translation="MKTTCALAALLMLFPAAMGWTEEPAIDLEAMMAEMPKPTAEHEW
                     LKQLVGEWETEATITMGPGAEPITHTGTETVEAIGGFWVMGRNEGICTLDGSPFTGIM
                     TLGYDPAKEKYVGTWVDSMNSRLWTYEGTVDAAGKTLTLETEGMSPANPEETCKFRDV
                     MELKGRDERVFTSSFEGPDGEWMKMVAIKSRRVK"
     gene            complement(14637..15506)
                     /locus_tag="PLS90_04765"
     CDS             complement(14637..15506)
                     /locus_tag="PLS90_04765"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK01749.1"
                     /translation="MATRKNEPNINFDRELTKGQRTYDRAVGEWWLDRSTDGAHTRAY
                     RKIARQIRERIAREPREIIDYACGPGSLLVRLARQFPESRLIGIDGSRKMVEAARARL
                     AAQGRPIAARTEILYSNLPNFALPLQADLVIFAFPNICPNADVQPYYDRHGARHREDV
                     KVARALAKAREPDPEDETVTQTPEELFMALIDAKVISRNLRKLLRKGGTCVRIEYANA
                     PREEMTDLVRMRLDFEAGSLARAFGGARAERLFRLREVSYTRSRVIEDVYHQTRDEGD
                     RVGGYLISTLTAI"
     gene            15911..17869
                     /locus_tag="PLS90_04770"
     CDS             15911..17869
                     /locus_tag="PLS90_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01750.1"
                     /translation="MPPSAKRRNRRRAASAPHPAAPASALQFLAAGGDALRRGDAVRA
                     DELFTRAIAAAPDHPAAWHGFGLAALTAGRAPDAAQRLQRAAQLAPRDAQLRFHLGVA
                     CQRAGRLEEAVAAFLDAFNLSNDHAAALANAGILLHSVGDVADALEFYRSPAGAPFAG
                     SGEHARLAAIHALQTRYARAMAAWRASGPAAADALLARLETGRWCPVVPQSVEAIDRW
                     RSDFAAWLAQARPLHWHGRDAELNAVYPLPIFYLPYQGRDNLDLKRRFAALFDLAAPA
                     PTPNSDAPVRRIGLHVGSSHAGIFRKHWAGLLDRWTRDDLPLTILTDPAEIDPLRRAI
                     ANPRIRFLRLPADFMAAVHAIRQQAFDLLYFWEVGSDSSNYFLPFFRLAPVQCTSAAL
                     PDTTGHPAVDWFVSWADSERPDPDHSYTERLFLPRSMPRYYRRPGRPDGALPARDALG
                     LPAHGPLFLCAQPPIKCHPDFDPLLAGILRGAPDARIVLFAGNPAPLTDLLVTRWRRV
                     MPELLDRILILPRRPLADYLAIVRRCDVVLDTPHYGGGTTMFEAFALGQPVVTLPGEF
                     MRGRFAAGCLRRMGIDELIARDPADYVRIALRLALDPGYRGAVASRIARASDCLFEDE
                     ACIAEHERFFEQALGLRHAQPPLEALAS"
     gene            17866..18594
                     /locus_tag="PLS90_04775"
     CDS             17866..18594
                     /locus_tag="PLS90_04775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01751.1"
                     /translation="MTDWARHWIEFPLTCLPGDHLRQVGKTVGGQPISAAQVGVIADS
                     IVGHLHLTAGDRLLDVCCGNGLLTRILADHCAAATGVDFSPPLIAVARRDHLAANIRY
                     HLMSALALDAPTLLAEGPFSRLLVYEALQHFTPRDLDPLLDGLLALAAPRFRLLLGGI
                     PDRRRKWRFYDTPRRRLDYLRRRLTGREAIGTWWDPATLARAAARRGLACRILPQDPR
                     LHTAHYRFDVLIENDAAPAEAAGV"
     gene            18587..19396
                     /locus_tag="PLS90_04780"
     CDS             18587..19396
                     /locus_tag="PLS90_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPK01752.1"
                     /translation="MFEHVRVLVGMPRSGTSWLSQIIDSCPDVRFRLSPIFSYAFKNC
                     VNEQSARDDWEAMLRGAYELDDEFMTQARNRRAGHYPSFPDKATAPRFLAIKDTRFHN
                     LLRPMLGHFPELKLAAVVRDPRGAIHSWLTAPREFPPDADPLLHWRSGACRKHGFGEF
                     WGFDDWKAVTRLHRALAAEFPDRFLLVAYEDFVAEPFDQAAALFQFMDLPWSPQTERF
                     LAACHQAHDDDPYAVFKNPAVRDRWRSELQPQIRRAIERDLAGTDLEVYLR"
     gene            19393..20109
                     /locus_tag="PLS90_04785"
     CDS             19393..20109
                     /locus_tag="PLS90_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPK01753.1"
                     /translation="MKLGIMQPYFFPYLGYFSLIRRTDQWIVFDTAQFIRHGWVNRNR
                     ILHPNDGWQYILAPVRKHSRETPIAQIELDNRQDWRGRLLGQLVHYKKQAPFYADTVA
                     FLEQALAFQTDSLAEMNVHLLAAVCRRLRIPFNPRFFSAMDLPLGPIAGPGDWALRIS
                     QALGAAEYINPPGGAALFDPDAFARAGIRLTINPYEPLVYPCGRYHFEPGLSVIDAML
                     WNPVERLADHLAAAPLPAAA"
     gene            complement(20294..20764)
                     /locus_tag="PLS90_04790"
     CDS             complement(20294..20764)
                     /locus_tag="PLS90_04790"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPK01754.1"
                     /translation="MSAAGEMASGPAGARVWPWGLLAARLIVGGLFLFAASLKIGNPT
                     AFGGAIEGFKLLDASITLEFGLLLIWLEILCGALLVLGIWSRPAALLIVGMLCLFFVA
                     LASAVVRGLEIDCGCFGALMQGGIGWGSLARTLVLLGLAVLVFRRGPGRFALGR"
     gene            complement(20761..21351)
                     /locus_tag="PLS90_04795"
     CDS             complement(20761..21351)
                     /locus_tag="PLS90_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPK01755.1"
                     /translation="MMPPAGRIAVQAAVLLLLAAAAAAIHRTRVEEAPRIVLQAEETR
                     PAADGVTTPTQTAAATPGGGTGGAPAAEAGEGVSVVGVVTPERALELYNTDYVQFVDA
                     RNPADYAAGHLPGAVNLPFGEFGRGKPGNLDELIPEAMTIVYCEGGECESSRLVAQQL
                     VMLGFVDVKIIEVGYPGWVAAGLPVESGDSSTGANR"
     gene            complement(21348..>21922)
                     /locus_tag="PLS90_04800"
     CDS             complement(21348..>21922)
                     /locus_tag="PLS90_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01756.1"
                     /translation="IWARLIDVRPTPAPAGQLDGETSTTTEARIAVGIKPGGETGTLQ
                     GRVLVRTNLEQQPVVEIPVLGLITVPYVVSPTILFFGVMAPATTVERTLVLQSMDGEP
                     FEIERVDMSGLPIEWKLIEQTEDVSTPDRDPRSIKRLQFSFTAPEDFEGLEDEIQIIT
                     DREGNNIIPVRTIARSAKPGEMLDDALGGE"
BASE COUNT         3403 a         8892 c         5885 g         3642 t
ORIGIN      
        1 ccgccgccat ccggcgtcac ggcgccgcgc tccgcgccat ccgcatcggg cgcgtcgccg
       61 acgagttgac cgccatcctg accggccccc gacccggccg cgccatgcac ctgatggacg
      121 acctcggcct gtggacccac gtcatccccg aaatcgaacc catgaaaggc tgcccgcaac
      181 cccccgagtt ccaccccgag ggcgacgtct tcgtccacac cgccctcgtc ctcgacctgc
      241 tgcacgaggc atggggcggc gcgcctcccc ccgagctggc ctgggccgcc ctcctccacg
      301 atgtcggcaa accccccacg ctgacgcacg acggccgcat ccgcttcccc gaacaccagc
      361 gcgtcggcgc cgcgatggcc gaccagatcc tccgccgcct gcgcctcccc aaccgcatcc
      421 gcgaaatcgc cgtccagctt gtcgccggcc acatgcgctt catggacgtc gccgacatga
      481 agcagagcaa gctgcgccgc ttcctcggcc agccccattt cgaccagcac ctcgccctgc
      541 accttgccga ctgccgcggc agccacggca aactcgacaa ctaccgcttc tgcctcgacc
      601 aggccgagcg cttccgccgc gacgacgccc aggccgccct cctgcccgag cccctcctga
      661 ccggccacga tctgatcgcg ctcggcctga agcccggccc cgcgttcagc cgcatcctgg
      721 aggccgtccg cgacgaacaa ctcgaaggcc acctcgccga ccgcgacgcc gccctcgcct
      781 ggctccgcga ccatcgggcg gatttcgaaa ccatctgaat cccgccggca acgcccccac
      841 gccccgccgc tccccccaaa cttgaatttc caatttcacc cccgctgtgc ttacctcttg
      901 gggggcagca gacgccatgg gccgcaaacc aacctcaccc cctgaaccgg cgccggatca
      961 cctccgcgcg atcgtcgaac gcttcgccgg ccggcgcgtc ctcgtcgtcg gcgacgtcat
     1021 cctcgaccgc tacctctggg gcgacgtcga gcgcacctcg ttcgaggccc ccgtccccgt
     1081 cgtcaacctc cgcgccgaaa ccgaccgccc cggcggcgcc gccaacgtcg tcgccaatct
     1141 ggccgctctc ggcgcccgac cccagctcgt cggcgtcgtc ggcgccgacg gcgagggccg
     1201 cgccgtccgc gacctcctgc gcgcaatgcg cgtctccccg caaggactcg tcgtcgaccg
     1261 cgcccgcccc accatcgtca agacccgcgt catcgcccag cgccagcagc tcatgcgcct
     1321 cgaccgcgag gagacggcgc ccatctctcc cgccgtcgcg cgcgcgctcg tcgcccgcgc
     1381 ccgcaaggcc ctcgccggcg cggcggtcat ggtcctctcc gattacgcca aggggacgct
     1441 cggtcccgac gtctgccgcg agctgatcgc cgccgcgcac aacgccggcg ccctcgtcgt
     1501 cgtcgacccc aaggggaccg atttctcgaa atatcgcggc gccgacatcg tcaaacccaa
     1561 ccggcgcgag gccgaagccg ccagcggccg ccccatcgat tcccccgccg cgctcgacgc
     1621 cgccgccgcc gccctgcgcc gcgccgtccg cggccgcgcc ctcgtcatca cgctcgacgg
     1681 cggcggcgcc gcgatcttcg aatcaggtcg ccccaccgtc cgcaccgccg cccaggccct
     1741 cgccgtcttc gacgtcaccg gcgccggcga ttccttcatc gccgcgatga gcgccgccct
     1801 cgccgccggc gccggcatcg ccgacgccgc ccgcctcggc aacctcgccg gctcgatcgc
     1861 cgtcggcaaa ctcggcgccg ccaccgtcga accggccgaa ctcctccgcg ccctcgaccc
     1921 cggcgccggc caccgcaagc tccgctcatg cgcccagttg ctcgccgacc tcgcccccca
     1981 ccgcgccctc ggccgccgca tcgtcttcac caacggctgc ttcgacctga ttcacctggg
     2041 ccatctcaag ttcctcgaac aggcccgccg cctcggcgac ctgctcgtcg tcgccatcaa
     2101 ctccgacgcc agtgtccgcg ccgtcaaggg cgccccgcgc cccgtcctcg gccagaacga
     2161 acgcgccgcc atcctcggcg cgctcgaggc ggtcgatttc gtcgtcatct tcgacgaacg
     2221 aacccccgaa cgcctcctcg aacagatccg ccccgacatc ctcgtcaagg gcaaggcgct
     2281 gcgcgccggc cagatcgtcg gcgccgacct tgtccccgcc tggggcggcc gcgtcgtcag
     2341 cctccccctc ctcggcgaaa tgaccaccga ccgcctcctc gaacgcatcg cccgccaatc
     2401 ccgcccctga atgctcagat cgtgtcagga ttcgtatcgc aatcggtatc ggtatcgtaa
     2461 tcgctgtcgc aatcggtatc gttcccggcg cagccccccg cccccgccgg ccatctcctc
     2521 ctgtgctgtt gtcgcactgg cctcccccgg tcattcccgc attatcccgc tcagaaattc
     2581 ggccctcggc tctcgtcctt cgtcctcggc tctcgattcc ccccgcatcc cgcagggatg
     2641 ccggacgcgc ccgccttatc ctaacggaac aatttcgtac cagcttcttg gagtgcggcg
     2701 ccagagcgtt gccacgcaac gcgccgacgc cgctttggat ccgacgttgc agcgccgtct
     2761 tcttccagcc tggaaccttc caatttgttt cgattttcga atttcgaatt tcgagtttcg
     2821 cgtagcgttc ttcccgtccc cgccctcact cccccccgag cgcctcgtgc caatccggcg
     2881 gcagctccat ccggtagagc tccagattgt cctgcgccat ccagttcgcc ccccagtaaa
     2941 tattgttccc cgcgtggtcg atgctcgcga acgcctcggc gaaataaccg ccgacgtcct
     3001 cgctcccccg ccaacggtgg ttcaacgtcg accccagccg ccacacccgc ggatgcagcg
     3061 cgtaatgctc gatctcgatc atcaggagct gattggccgc ccaccggtca tcaaacggcg
     3121 tatacgttga catcagaaac cagccgcgct tctccggcgc gcgcagccgc ccgatgtgct
     3181 ggtgcagcgg ataccccgcc tcggccatgt tgaagatgtc gatccggtcg cccgactccg
     3241 gctcgaacgc cgaaaaccag tcgttcgtgt tgtcctggaa caccagcacc tcgcgcccct
     3301 gcttgtccag cgcccagccg ttatgcccca tcgaccgcgt cggcaggtaa actggccggc
     3361 tggtgtcgcc ctgcgccagg tcggcgatcc ggtaaaaccg cggccccagc gccggatcgc
     3421 cgttggtgtg ctccagcacc acgaaccagc ggccgctggg gctgatgtcc agcgcgttgg
     3481 gcttctgctc gatccagccc ggcagcacct tgtcgcggtc cttgtcgtag acgacgcacc
     3541 gcccgtcggc cagacggaac gcccagtagc gcgcgtcgtc cgacggcgcc atctcggccg
     3601 tcggcacgat cggcgtgatg aaatcgtgca gcacttccgc cgacgccggc ccctgcacgg
     3661 cgtcctggcg gtagaacagc ggcccgacgt ggtaatagat cgtcgtctcg gtccccggcc
     3721 ggccgctcag gtcccagcgc acctcgtgcg tttcgcccag cggccggccc ggcgcggatt
     3781 caaccgggcc gagcagcgcc aggtcgctca gccgcaccag gtagctctgg ttgtcggtgc
     3841 ggaaggcgat ggcgtagcgt ccgtcgacgt tgacgttggt ccagcgcgcg taaccgttgt
     3901 agaaaccgcg gtcgggcgac tcggcgaacg ggccgcggcc gacgtcgcgc acgcgctcgg
     3961 gccgcagctc gccgatctcg gtcagccgcg tgatccggtg gcccgtctgc ggctggatga
     4021 acggcgcccc cttgggcggc ggcgccgccg ccggctggcc gaacaccagc ttgctcgtcc
     4081 gctccagcgg cacgttctcc agccgctcga cgagataccg gctcccggcc ggctcgcgcg
     4141 cggccgccgg cgcgccctcg ttcaccgcgg ccgcagcggc cgtgggcgtc gcagccggcg
     4201 cgggttcggc cccggtatcg gcgtccggcg tttccaccgg cagcggctgc ggcgtcgcga
     4261 tcgcgccggg cagctccacc gccggcgtcg gtatcgctgt cggttcagga gtgggcgtcg
     4321 ccgtcggttc agccgtcggc tcgggtttcg ccgttggcgt cacggctggc ttcggcgtcg
     4381 ccctcggcgt cggctcaggt gtcggcgcaa gtgtgggcct cggctccggc gtcggtgttg
     4441 ccgtcggctc aggtgtcggt tcggctctcg gcgtgggctc aggcgttggt tccggcgtcg
     4501 gttccggcgt cggcgccgcc gccgcgatca cccgcaactt cacatccacg ctccagccgt
     4561 ggatcccgtt gtcatacaga atccgccacc aggtatagtt ctcggcgcgc accggaccgc
     4621 ccatcaccgt ccccgccgac cccggcttgc gcaccgcgat cggccgcgcc gccatcgacg
     4681 gccgcggccg cacgttcacc cgcgccgtcg tctcgatccg gctgccgacc tggatctcgg
     4741 cggcgccgac aacccccgcc agcaaccccc acgccacgca gacgcccagc gtcagccggc
     4801 gcaaagtcgc gaggcgatac ggaacagggc ggaccatggg cggatggctc cacacgcgcc
     4861 aaaatgcggt gacggctcgt cgcctatctt aacggaaacg gcgcggcgcg gcttgagcct
     4921 actcgtagag cgcgtgcttc aacaccccga tgcacggcag gttgcggtag cgctccttga
     4981 aatcgaggcc gtagcccacc acgaaaacgt cgtcaatgtc gaaacccagg tagcgcaccc
     5041 ccagcttctg cacctgcggc ttcgtcttgc gcagcagcgc gcagatctcc aggctggccg
     5101 ggttgtgcag ccgcagcaga tcatgaatga cgtgcagcgt gttgcccgtg tcgtaaatgt
     5161 cctcgacgag cagcacgtcc ttgccggcga tgctctggtc gacgtccttg gtgacgcgca
     5221 cgccccccag cgccacggtc ccccccttgt agctcgacgc ccccaccagc tcgatcgcgt
     5281 ggggaatcgt gatccgccgc atcagatcgg ccagaaagac gatccctccc ttcagcacgc
     5341 tgatcaggac cagatcgcgg ccggcgtaat cgcgcgaaat ctccgccccc agccgctcaa
     5401 tccggtcggc gagctcccct tcgctgatca gcacccgctc cagatcctgc aacagatcga
     5461 cgggattcgc ggtcaccttc tgcggctgat tcatatcccc tccaacatca tttctcggcc
     5521 ctcatcctca ttctaaaatc gtccctcgtc ctcggctctc gaatgcctcg tttgctttcc
     5581 agttccccgt tctcatcccc gccctcttgg agtgcggcgc cagagaccgc gcagcggtcg
     5641 tcgacgccgc tttggatcag tcctgatccg cccgcttcat cggaaccaaa gcgatctcgc
     5701 ccccgggccg gtgtgggacc ggcgcccttg ccggtccgcc cctgttttcg cgccctttcg
     5761 tgccgttcgt gatccaaaaa cctcttccct tggcctcatt tccccagata cgtccccgtc
     5821 cccgtcgcgg ggaaatgcgt caccttgccc ggcaacccgt cgaccatctc aatctgcagg
     5881 ccgtactcga tccattgcca cgtttcgtac atccggccgt tgcgcagcac cggcggcgtc
     5941 cgcgaactcg gatccccata gcgcgcgacc agcttgatca cttccgacat cggttgcacg
     6001 cggtaactct gcccgtcgtt caggaacagc acctcctgct ccagcgccgg gtagagccac
     6061 gagcgctgca tcacctgcag cgtttcgggc acctggtcaa tccgcccgct caggtccgaa
     6121 ctggtaatca gcgacccgtc ctcgcgccac cagagccgga aaaaatcggg cttccccatc
     6181 cgctgcaaca cgtccgcctc ggccggtccc agcggctggt tcgccggcgg ataccagtcc
     6241 ggcacccgca ccagattcat cgagaaccgc cggtcgatct cgatctgata cttgttgcgc
     6301 gagcaaccgc ccgccgccag cccgccgagc agcagcgcca gggccagcac aagccgcgga
     6361 tgaaacaaac tttccatggt ccggtttcgc cagaaaatat gctggcaccg gtcaggcgcc
     6421 aggtccaacg cctgcccgac actccgcgat ccaacatacc cacatcgcgc cggggtgaca
     6481 agtcatctgc gccggcctgc agcgcgcgac ccgcaacttc cccgcatcgc gccggggtga
     6541 caggcctcgc acacccaccg gcacggaacg gccccattca aacccgcccc ccccatcgat
     6601 atcggtatcg caatcgccat cgctatcggt ttcgctatcg ccatcgcgaa tgcaatcggt
     6661 atcggaatcg atgcaccccg cgcacgcctt gccagcccgc gccagcgggc tttctccggc
     6721 ccccgcgggg ccttgtcatt tgcaccgggc ctcggcccgg tgacccaatg cgaaatattt
     6781 tccctaaacc gtttcaacgg tttcccccgc aacgccgcca atcccacgtc acccgccgct
     6841 caatcctccc aggccggtgt gggaccgccg cccacgccgc ctcccaacca ccgtttgaca
     6901 atccgcccgc cgaatcctac gatgcggtag gattcgtgta gggaggtcac cagaatgaag
     6961 gaaactgccg acggaacccc accctccggc ggcgccacgc ccttgggatc agtccgccgc
     7021 gaggtccgcg aactctccct cctcttcgaa atcagccaga tcctcgactc cgccatcgac
     7081 ctgcgcgagg tcgtccagca ggtcctcatt gccatctcca accacatggg catgtcgcgc
     7141 ggcaccctcg ccctgctcaa ccgcgacacc cgcgaggtga tgatcgagac cgcccacggc
     7201 ctctccaaca gccagaagga acgcggccgc tacaagctcg gcgaaggcat caccggcaag
     7261 gtcatcgaaa ccggccgccc gatgatcgtc cccgacatcg acgccgaacc cgaattcctc
     7321 aaccgcaccg gcgcccgccg ccggcgcgac cgcaaaacca tcggcttcct ctgcgtcccg
     7381 atcaaacagg gccaggaggt ctacggtgcg ctcagcgtcg accgcttctc cgagggcggc
     7441 gtctcgctcg acgaagacct ccgcctgctc cagatcatca gctcgatgat cgcccaggcc
     7501 gtccgcctcc gccagtcgct ccgcgaggaa cgcgaacgcc tcctcgccga aaaccagcgc
     7561 ctccaggagg aactccagga tcgcttccgc ccggccaaca tcatcggcca ctccaacgtc
     7621 atgcagaacg tctacgacct catcgcccag gtcagcaaga gcaacaccac cgtcctgatc
     7681 cgcggcgaga gcggcaccgg caaggaactg gtcgcccacg cgatccacta caacagcctc
     7741 cgcgccaaca agcccttcat caaggtcaac tgcgccgccc tccccgagtc ggtcctcgaa
     7801 agcgaactct tcggccacga gaagggggcc ttcaccggcg ccatctcgat gcgcaagggt
     7861 cgcttcgaac tggccgacgg cggcacgatc ttcctcgacg aggtcggcga cttcaccccc
     7921 gccacgcaga tcaaactcct ccgcgtcctc caggagcgcg aaatcgagcg cgtcggcggc
     7981 agccgcacga tccccatcga tgtccgcgtc atcgccgcga ccaaccgcaa cctcgaacag
     8041 atgatcgaaa ccgccgaatt ccggcaggat ctctactacc ggctcaatgt cttccccgtc
     8101 cacatgccgc cgctgcgcga gcgcaagagc gacgtcctcg aactcgccaa ccacttcatc
     8161 gaaaaataca gccgcgccaa ccacaagaac atccgccgca tctcaacccc cgccatcgac
     8221 atgctgatgg cctaccactg gccgggcaac gtccgcgaac tggaaaactg catcgagcgc
     8281 gccgtcctcc tctccaccga cagcgtgatc catggccatc atctcccccc gacgctccag
     8341 accgccgagg ccagcggcac caccttcacc ggctcgctcc ccgagacgct cgacaacttc
     8401 gaacgcgaac tgatcctcga cgcgctcaaa tcggcccgcg gcagcatggc caaggccgcc
     8461 cgcgccctgg gcctcaccga acgcgtcatg ggactccgcg tcaagaaaca cggcatcgac
     8521 ccccgccgct tccgcatgcc ccgctccttc gcctgacccc cgcccccctc tccaattctc
     8581 gtcctcatcc ccgagcccgc ccgccccgga gcggcgccat acgaatagcc caagctggag
     8641 cgcagcggga cttggagcta gccatccaaa caatcccgtc cccgaacggg gcgaaccccc
     8701 ggccactgca gagcaagacc gcgccgagga acttgagaag ctgattcatg accagactta
     8761 cgatcagctc gaagatgatt cgaagtgtcg ccacaccgag ccggcccggc agccgccccg
     8821 cctgcgagag cagcgccagc tcgatccgct ccttctgcag cgcgtgcagc gcctgggtcg
     8881 tcacctggat gttcccatag agcagcaccg tgctcagccg gtgcagctcg atctcctcca
     8941 gttgcacgct ttccttctcg atgaccagcg tcaggccgcg cttgcggatt gtcgcgccct
     9001 gttcggtcac ctaaaggcag cccatccgct tcccctcccg gcacatgggc ttacgccctg
     9061 gtggctctca gacatggatc ttgtcacggc ttgccgaccg tcttaatccc atctgcaact
     9121 gggaattggt tctgacgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccc
     9241 atctgcaact gggaattggt tctgacgagg gggcggaacc gggcgatcca gcctttggcg
     9301 tcacggtagg cggaggccat cggggagttg ctcagtggat cgagatgacc ggcggttctc
     9361 ggttgtcagg cggcagcgag gcggcctccc ttccgatgcc cgagccgtta agcctggcga
     9421 tgatctgctg agcgatgaag tcgccgattg gggtttccag gtgatcgacc aggagcgggg
     9481 tgacatcctc gtcatagttg atgaatacaa acccgccgac cagggcgatg acgccccact
     9541 ccagccagct tatctccgtc caggtccggt ccatgtcggc atggcggacg ttcagctcga
     9601 ccggcaccgc ccatgaatca atctgcgtgt tcctggcggc gtcggtgagg ctcacctgca
     9661 tgtcgtaatc gacgttgtag tgatagccat gccaggcggg ggcccagacg agaaagccgg
     9721 ggaaattgat gagaaaattc cagcccgagc cccggtattc ggggcggacg gcaatgttgg
     9781 cgatcacgtc tggcttcaag gcgctgccga cggcataggg catgatgacg gtcgccccgt
     9841 ggcgctccag ggccgtgccg atgcccttga tcagcttctc ggattcgata tccgtggttg
     9901 acggcacaat ccccacggtg atgggcgggt tgtgcagcgg ctgaatctgc atgctttcgt
     9961 aggaccgcag attcctgacc tccaaggcgt gcgagcagcc caggagaagc acggccaggc
    10021 atgccaggcc ggcaagcgga taccatttca tagttcccct cctgattgat ctgttggcgt
    10081 cagagggacg ccgaaaatca cgcgggcgaa ccaggctcgt tcagccgcga ttgctcgcgg
    10141 gaagtcggct ccacctcgcc gcgaaaatgc gttcggatca tcgcgccgaa actgttgtcc
    10201 gagtccacgg agccccggat gcggtgcttc tgcccatcgc gttccagact cagcggcatg
    10261 agcggaaaat cggcggtctt cggggccttg agttggtccc ggacaaagcc gcgcatcatg
    10321 gtccacgcca tgctgctcgc gtcgtcggag cgccaggcgt caggattatt gatatggcgc
    10381 aggttttctt cggcgctgcg ggtggtgacg gaatgaaagc acgcgcccat catcacaatg
    10441 atgatcagca ggcaacccca cgagccgtag ctgcccggcg atttcttgat cggctcgccg
    10501 caattcgggc aggcggcggc attcctgctg accccgtgac cgcaggcgcg gcattgaatc
    10561 agggccatcg gatcatccct tctttgcctc ctgccgcttt ttcgcgacct ttgcggcctc
    10621 ttcaagcggc tgggcggcca ttctgaggat caatctgagt tgcaagtcgt tcccatgcag
    10681 gcgcggaatt gtctcgcggc tctcccgttc aatcgcctgc acgcgctcaa gcggcaagtc
    10741 caaatgtggc actaccggca gaaattgtca attcgcaacc tgggttctca aatttcaggt
    10801 ccgcccatct ctcactgccg catgtgtcgg ctaaaaaaac tcccggccgg attaagccga
    10861 ccaggaggtt caacttgcaa aactgccagc aaactgccga actcgggcag gaataaggcg
    10921 gcatcagcgc cggatcgtgg agtgaaagtc gaagactcga cttgacgaaa acccctgaaa
    10981 acattgatga tattcaatgc tggagggatg accgagtggt tgaaggtacc ggtcttgaaa
    11041 accggcaagg cgcaagcctt cgtgggttcg aatcccactc cctccgccac cccccccacc
    11101 caatcgtccc tcgtcctcgt gatcgcccca aagacccctc caaaactcgg ctctcggctc
    11161 tcgtccctcg tcctcggctc tcgaaaaccc gccatcctgt cccttcgaca taagccgccg
    11221 ttcccagggt agcccgtcct cttcctcctc ccctccgaaa cccctacctc ctccccccgt
    11281 tgcgcgccat cagcgccaga aacggacggt tctcgtccgg gtcgacatgc gtcgccggcg
    11341 ccttccaccc cgccgcctcc accagccgct ccagcgccgc cgccgtgaaa tcccacagat
    11401 gctccttgtt cgcataaccg cgatgccgcg ggtcccacgt gtaacgccgc ccgtcgggca
    11461 cctccacgta gagcccgccc cccgcggccg catgccgccg cagttccgcc agcagcccga
    11521 ccggatcggg aacatgttcc agcacatggc acagaaaaat cagatcgaac ggcgccgatg
    11581 ccgcaatcag cggatgctcc gcgaaaccgc aacccaacgc ggcccgctcc tccccggcgc
    11641 ccgccatccg ccgcaccagc ccgcgcgcaa acgccacgtt ctccgccgac ggatcgacgc
    11701 cgacataacg ccacccgcgc tcgagccaga agatcgcgct gcgcccgttc ccgctgccgt
    11761 agtcgagcac cttcggcccg cctccgtccc cggccgcgcc ctcccccgca tcgttccacg
    11821 cgccggccgg ccgcagcgcc gccagatacg cctccacccg ctcgccgttc tcccgcacgt
    11881 gctccgcgaa cacgccgtcg ccctgataca gcccgtggcg cggatacgcc gcatcgtaaa
    11941 acgccgccag gctgtcgccg gtctccaggt gctccggcgg cgtcagcccc atctggcagt
    12001 cggggcagaa atcgatcggt cgcggaaccg aaaacacccg gaaccgccgc gcatgccggc
    12061 acggcccctc gtcgggcagc cccggcgccc cggtcagatg cggccgcacg cgccgcgcga
    12121 tccgctcccg ctccagccgg tccgcccagc ggttcagcgg actcagcacc ggcagcagca
    12181 cccggcgtag caggcacgcc cccggccgcg cgtcccacag cttcaggtcg cgatacagaa
    12241 accacgcgcg cgcgcgccgg tcgcgcagcg ccgaccgcat gaacgcccgc cgcgccggcc
    12301 gcagccggcc cagccccgcc gccgtcgcct ccggctgcgg gctcagcagg atccgcgcgt
    12361 ccgccgcccc gcgcgcgccg gcgtcatcca gcgcacgcac ctcgagccgc gcgatcgccc
    12421 gcgcatcccg ttgtgcatcc agcgccgcgc cgcccgccgc ttcctcggtg aatatcgtga
    12481 cgtccgatcc cggatggaac gtgccgaccg cgtcgagcgc cgccgccaac tgccgccgat
    12541 cccccgcccc gtcatacagc tggatgcgca gcggcgaccc ctgcggccag ctccgcaccc
    12601 gcgtccgctc gtagaggctc accagctcct cgcgcagccg cccaatcagc ccgatcaatc
    12661 cccgtccccc ttgcttcgtc cctcagccct catgcaaata cttgtcgttc cccaaatcgc
    12721 aagctgcata aggattccaa cttcaatgca ggcggttctg acttgtcggc cgaattaagt
    12781 ccgcagactc gtcctcggct ctcgaactcg tcccccgccc atatggagtg cggcgccaga
    12841 gaccgcgcag cggtcgtcga cgccgctttg gatcaggcgc gggaccggcg cccccgccgc
    12901 cgtgaacccc cgcccccgcc ggtggttcgg tccggcgcgc gcggccgccg cggccaccac
    12961 gcgcgccgac tctcaccgat ggcgctaagg cttctgggcg aacatctccc gcgcgcgccg
    13021 gtccagttcc tccggcgcca cgtcctccac gtgcgtcgcc agcgaccaga catggccgaa
    13081 cggatcttca agctgccccg tccggtcgcc atagaacatg ttcatcaccg gcatgcgcac
    13141 cttcgcgccc gccgccaccg ccctctcgaa cgccgtatcg acatcctcca cgtatacata
    13201 caggttcacc ggcgtgccgc ccagcgtctg cggcgatttg ttgccgtatt ccaggcattc
    13261 gccgccgagc atcaccgccg aatcgccgat gcggagctcg gcatgaccca gccggccgtc
    13321 cggcgtcagc atcttgtaca tctcgcgcgc gccgaacgcc gccttgtaaa actccagcgc
    13381 cttgaccccg tcgccgcacg tcagatacgg cgtcaccgtg tggaaattct ccggaaccgg
    13441 tttgactgcc atgacatctg ctccttgctg tttccaacag ttttcattgg ctttcatcct
    13501 tgcggccgac cccctcagcc aattcccgcg gaccgcttgc cggactcgct tgaccgcgcg
    13561 tccgctgtta tttctctgcg tccttcacgc actccgcggt tcagtctgtt tgccttcttg
    13621 ccttatccac tttgcggatt attcccagtc tttgaatact agccattcga atttgtttcg
    13681 aatttcgaaa ttcgtatttc gtgctgcttc cccgtcattt cacgcggcgc gacttgatcg
    13741 ccaccatctt catccactcc ccgtcggggc cttcgaacga cgacgtgaag acgcgctcat
    13801 ccctgccctt gagttccatc acgtccctga atttgcacgt ctcctccggg ttcgccggac
    13861 tcatcccctc ggtctccagc gtcagcgtct tgcccgcggc gtcgaccgtc ccttcgtacg
    13921 tccacaggcg gctgttcatc gagtcgaccc aggtgccgac gtatttctcc ttcgccgggt
    13981 cgtaacccag cgtcatgatc ccggtgaacg ggctgccgtc gagcgtacag atcccctcgt
    14041 tccgccccat gacccagaac ccgccgatcg cctcgaccgt ctcggtcccg gtgtgcgtga
    14101 tcggttccgc ccccggcccc atcgtgatcg tcgcttccgt ctcccattcc ccgacgagct
    14161 gcttcagcca ttcgtgctcg gccgtcggtt tcggcatctc cgccatcatc gcctccaggt
    14221 cgatcgccgg ctcctccgtc catcccatcg cggccggaaa cagcatcagc aacgccgcca
    14281 gcgcgcaggt cgtcttcatg gtgattcgcc tcgctttctt ggatgtggtc cgcgggtgtc
    14341 gcttgccgct tccatcttag gacgaacggc gacgggaacg gagaataatc cgccaccaaa
    14401 actttttcgc gatgcgaatc atcaaatctt ggaatccaca ggtcctgtca tctcggctct
    14461 aaacgattca attcccgcgg ttgtggggga atcccgcagg gatgccaccg gtgtcggacc
    14521 ccggcggccc tttcaaccca cggtccgatc cgtcattttc aattgaactc aatcatcggt
    14581 ttgataatct agtttgttgc gcgcatcacc cccaccggtg cgggaccgcc gccccctcat
    14641 atcgccgtca atgtgctgat caaatacccc ccgacgcggt ccccctcgtc gcgcgtctga
    14701 tgatacacgt cctcgatcac ccgcgaccgc gtgtagctca cctcccgcag ccggaacaac
    14761 cgctccgccc gcgccccgcc gaacgcccgc gccaggctcc ccgcctcgaa atccagccgc
    14821 atccgcacca gatcggtcat ctcctcccgc ggcgcattgg catattcgat ccgcacgcac
    14881 gtccccccct tgcgcagcag cttgcgcaga ttgcggctga tcaccttggc gtcgatcagc
    14941 gccatgaaca gctcctccgg cgtctgggtc accgtctcgt cctccgggtc cggctcgcgc
    15001 gccttcgcca gcgcccgcgc caccttcacg tcctcgcgat ggcgcgcccc gtgccggtcg
    15061 tagtacggct gcacgtcggc gttggggcag atgttcggaa aggcgaagat gaccaggtcc
    15121 gcctgcagcg gcagcgcaaa gttcggcagg ttgctgtaaa gaatctccgt ccgcgcggcg
    15181 atcggccgtc cctgcgccgc caaccgcgcc cgcgccgcct ccaccatctt gcgcgacccg
    15241 tcgatcccga tcagccggga ctcgggaaac tgtcgcgcca gccgcaccag caggcttccc
    15301 ggcccgcagg cgtaatcgat gatctcgcgc ggctcgcgcg cgatccgctc gcggatctgc
    15361 cgcgcgatct tccgatacgc ccgcgtatgc gccccatccg tcgagcggtc cagccaccat
    15421 tcccccactg cccggtcgta cgtccgctgc cctttcgtca gttcacggtc gaagttgatg
    15481 ttcggttcgt tcttcctggt cgccatgcct cgtctgctcc gccgtcgaat atggaggcgc
    15541 atatcattcc agagacccct ccccggagca atccccatca cacccatccg tccctcatcc
    15601 ctcagcctca ggagtcgccc ccaccggccc tcccatgatt cagcgctcgg ctcccgtccc
    15661 tcgtcctcgg ttctcgaaaa ccggccatcc ctcctcctcc tttcctcctc tccccctctc
    15721 ctcctcttct cccctctccc gcccgtccct cccctcctca cctcaagccc cctcgttccc
    15781 cgtccgattc gttaaagggg cgcccactcg cccaccggca cgctccggca caccccggtc
    15841 cgtgccgcca cgttcgccgc gcgctgccgc gcgcccggct cccacttgct ttcggagaag
    15901 cggtccgccc atgcccccat ccgccaagcg ccgcaaccga cgtcgcgccg cctccgcccc
    15961 ccatccggcc gcccccgctt ccgccctgca attcctcgcc gccggcggcg acgccctccg
    16021 gcgcggcgac gccgtccgcg ccgacgagct gttcacgcgc gccatcgcag ccgcccccga
    16081 ccaccccgcc gcctggcacg gcttcggcct cgccgcgttg accgccggcc gcgctcccga
    16141 cgccgcccag cgcctccagc gcgccgccca gctcgcgccg cgcgacgccc agctccgctt
    16201 tcatctcggc gtcgcctgcc aacgcgccgg acgcctcgag gaggccgtcg ccgccttcct
    16261 ggatgccttc aatctgagca acgatcacgc ggcggccctg gccaacgccg gcatcctgct
    16321 ccactccgtc ggcgacgtcg ccgacgccct cgagttctac cgctcgcccg ccggcgcccc
    16381 cttcgccggc tccggcgagc acgcccgcct cgccgcgatc cacgccctcc agacccgcta
    16441 cgcccgcgcc atggccgcct ggcgcgcttc gggacccgcc gccgccgatg cgctcctcgc
    16501 ccgcctggaa accggccgct ggtgccccgt cgtcccccag tcggtcgagg ccatcgaccg
    16561 ctggcgctcc gacttcgccg cctggctcgc acaggcccgc cccctgcact ggcacggccg
    16621 cgacgccgaa ctgaacgctg tctacccgct ccccattttc tatctcccct atcagggacg
    16681 cgataacctc gatctcaagc gccgctttgc cgcccttttc gatctcgccg cgcccgcccc
    16741 cactcccaac tctgacgcac ccgtccgccg catcggcctc catgtcggct cgtctcatgc
    16801 cggcatcttc cgcaagcact gggccggcct gctcgatcgc tggacgcgcg acgacctccc
    16861 gctgacgatc ctgaccgacc ccgccgaaat cgaccccctg cgccgcgcca tcgccaaccc
    16921 ccgcatccgg ttcctccgcc tccccgccga tttcatggcc gccgtccacg cgatccgcca
    16981 gcaggccttc gacctcctct acttctggga agtcggctcc gattcctcca actacttcct
    17041 ccccttcttc cgcctcgcgc ccgtgcaatg cacctcggcc gccctccccg acaccaccgg
    17101 ccatcccgcc gtcgattggt tcgtctcctg ggccgactcc gaacgccccg accccgacca
    17161 ctcctacacc gaacggctct ttctcccgcg cagcatgccg cgctactatc gccgccccgg
    17221 ccggcccgac ggcgccctcc ccgcgcgcga cgcccttggc ctgcccgcgc atggcccgct
    17281 cttcctctgc gcccagccgc cgatcaagtg tcaccccgac ttcgatcccc tgctggccgg
    17341 catcctgcgc ggcgcgcccg acgcgcgcat cgtcctgttc gccggcaacc cggccccgct
    17401 caccgatctg ctcgtcaccc gctggcgccg cgtcatgccc gagctcctcg accgcatcct
    17461 catcctcccg cgtcggccgc ttgccgatta cctcgccatt gtgcgccgct gcgacgtcgt
    17521 cctcgacacc ccccattacg gcggcggcac aacgatgttc gaggcctttg ccctcggtca
    17581 gcccgtcgtc acgctgcccg gcgagttcat gcgcggccgc ttcgccgccg gttgcctccg
    17641 ccgcatgggc atcgacgaac tgatcgcccg cgaccccgcc gactatgtcc gcatcgccct
    17701 gcgcctcgcg cttgatcccg gctaccgcgg cgccgtcgct tcccgcatcg cccgggcttc
    17761 cgactgtctc ttcgaggacg aagcctgcat cgccgaacac gagcgcttct tcgaacaggc
    17821 cctcgggctc cgccacgccc agcccccact ggaggcgctg gcgtcatgac cgactgggcc
    17881 cgccactgga tcgaattccc cctcacctgc ctccccggcg atcacctgcg ccaggtcggc
    17941 aagaccgtcg gcggccaacc catctcggcc gcgcaggtcg gcgtcattgc cgattcgatc
    18001 gtcggccacc tgcacctcac cgccggcgac cgtctcctcg acgtctgctg cggcaacggc
    18061 ctgctgaccc gcatcctggc cgaccactgc gccgcggcga ccggcgtcga tttctccccg
    18121 cccctgatcg ccgttgcccg ccgcgaccac ctcgccgcca acatccgcta ccacctgatg
    18181 tccgccctcg ccctggacgc gccaaccctc ctcgccgagg gtcccttcag ccgcctcctc
    18241 gtctacgaag cgctccagca tttcaccccg cgcgacctcg atccgctgct cgacggcctg
    18301 ctcgcgctcg ccgccccccg cttccgcctc ctcctcggcg gcatccccga ccgtcgccgc
    18361 aaatggcgct tctacgacac gccccgccgc cgccttgatt acctgcgccg gcgcctgacc
    18421 ggccgcgagg ccatcggcac ctggtgggac cccgcaacgc tggcccgcgc cgccgcccgc
    18481 cgcggtctcg cctgccgcat cctcccccag gacccccggc tgcacaccgc ccattaccgt
    18541 ttcgatgtcc tcatcgaaaa cgatgccgcc cccgcggagg ccgccggtgt ttgaacacgt
    18601 ccgcgtcctc gtcggcatgc cccgctccgg cacgagctgg ctcagccaga tcatcgacag
    18661 ttgccccgac gtccgtttcc gcctctcccc gattttttcc tacgccttca agaactgcgt
    18721 caacgagcag tccgcgcgcg acgattggga ggcgatgctc cgcggcgcct acgaactgga
    18781 cgatgaattc atgacccagg cccgcaaccg ccgggccggc cattacccca gcttccccga
    18841 caaggcgacc gccccccgct tcctcgccat caaggatacc cgcttccaca acctgctgcg
    18901 gccgatgctc ggccacttcc ccgaattgaa actggccgcc gtcgtccgcg acccccgcgg
    18961 cgccattcac tcctggctca ccgccccgcg cgagttcccc cccgacgccg acccgctcct
    19021 gcactggcgc tcgggggcct gccgcaaaca cggcttcggc gagttctggg gcttcgacga
    19081 ttggaaggcc gtcacccgcc tccaccgcgc cctcgccgcc gaattccccg accgcttcct
    19141 cctcgtcgcc tacgaagact tcgtcgccga accgttcgac caggccgccg ccctcttcca
    19201 attcatggat ctcccctggt cgccccagac cgagcgcttc ctcgccgcct gccaccaggc
    19261 gcacgacgac gacccctatg ccgtcttcaa aaaccccgcc gttcgcgacc gctggcgctc
    19321 cgaactccaa ccccagatcc gccgcgccat cgagcgggac ctcgccggca ccgacctgga
    19381 ggtctatctg cgatgaagct cggcatcatg cagccctact ttttccccta cctcggctat
    19441 ttcagcctga tccggcgcac cgaccagtgg atcgtcttcg acaccgcgca gttcatccgc
    19501 cacggctggg tcaaccgcaa tcgcatcctc caccccaacg acggctggca gtacatcctc
    19561 gcccccgtgc gcaaacattc gcgcgagacc cccatcgccc agattgaact ggataatcgt
    19621 caggactggc gcggccggct cctcggccag ctcgtccatt acaagaagca ggcgcccttc
    19681 tacgccgaca ccgtcgcgtt cctcgaacag gcgcttgcgt tccagaccga cagcctcgcc
    19741 gagatgaacg tccacctgct cgcggccgtc tgccgccgcc tgcgcatccc cttcaacccc
    19801 cgttttttct ccgccatgga cctgccgctt ggacccatcg cggggccggg cgattgggcc
    19861 ctacgcatca gccaggccct cggcgccgcc gaatacatca accccccggg cggcgcggcc
    19921 ctgttcgacc ccgacgcctt cgcccgcgcc ggcatccgcc tcaccatcaa cccctacgag
    19981 ccgctcgtct acccctgcgg ccgctaccac ttcgagcccg gcctctcggt catcgacgcc
    20041 atgctctgga accccgtcga acgcctcgcc gaccacctcg ccgctgctcc cctccccgcc
    20101 gccgcctgaa acgactttcg aactgatccc tcgcccctat ggagtgcggc gccggagacc
    20161 gcgcagccgt cgccgacgcc gctttgaatc gctcgccttc gcccatgttc gtcgaaacca
    20221 acccggccgc cccgcgttgc cactatggga ccgccgccac caccgatctc ggaccgccgc
    20281 cgcctgtgtc gaatcaccgc cccagcgcga accgtcccgg cccccgccgg aacaccagca
    20341 cggccagccc cagcaacacc agcgtccgcg ccagcgaccc ccagccaatc cccccctgca
    20401 tcaatgcccc gaaacacccg caatcaatct ccagcccgcg aacgaccgcg ctggccagcg
    20461 ccacgaaaaa cagacacagc attcccacga tcagcagcgc cgccggccgg ctccagatcc
    20521 ccagcaccag cagcgccccg cacagaatct ccagccagat cagcagcaac ccgaattcca
    20581 gcgtgatcga cgcgtcgagc agcttgaacc cctcgatcgc gccgccgaac gccgtcggat
    20641 tgccgatctt cagcgaggcg gcgaacagaa acagaccccc cacgatcagc cgcgccgcca
    20701 gcagacccca cggccagacc cgcgcgccgg ccggccccga agccatctcg cccgccgcgc
    20761 tcatcggttc gctcccgtgc tggaatcccc cgactcaacc ggcagcccgg ccgccaccca
    20821 gccggggtaa ccgacctcga taatcttcac gtcgacgaaa ccgagcatca cgagctgctg
    20881 cgccaccagc cgcgaacttt cgcattcgcc cccctcgcag taaacgattg tcatcgcctc
    20941 gggaatcagc tcgtccaggt tccccggctt gccccggccg aactcgccaa acggcaggtt
    21001 caccgccccc ggcagatgcc ccgccgcata atccgcgggg ttgcgcgcgt cgacaaactg
    21061 cacatagtcc gtattgtata gctccagcgc ccgctccggc gtcaccacgc cgacaacgct
    21121 gaccccctcc ccagcttccg cggccggcgc gcccccggtc ccaccgcccg gcgtcgcggc
    21181 cgccgtctgc gtcggcgtcg tcacgccatc cgccgccggt ctcgtctctt cggcctgcag
    21241 aacgatcctc ggcgcctcct caacccgcgt gcgatggatc gccgccgccg ccgccgccag
    21301 cagcagcagc accgccgcct gcaccgcgat ccgccccgcg ggcggcatca ttcacccccc
    21361 agcgcgtcgt ccagcatctc gccgggcttc gctgaacgcg cgatcgtccg caccgggatg
    21421 atattgttcc cttcccgatc ggttatgatc tgaatctcat cctccaaccc ctcgaaatcc
    21481 tccggcgccg tgaaactgaa ttgcagccgc ttgatcgaac gcggatcgcg gtcgggcgtg
    21541 ctcacgtcct ccgtctgctc gatcagcttc cactcgatcg gcagcccgct catgtccacc
    21601 cgctcgatct cgaacggctc gccgtccatg ctctgcagca ccagcgtgcg ctccaccgtc
    21661 gtcgccggcg ccatcacgcc gaagaacagg atcgtcggcg acacgacata tggcaccgtg
    21721 atcagcccca gcaccggaat ctccaccacc ggttgctgct ccaggttggt gcgcaccagc
    21781 acccgcccct gcaacgtccc ggtctcgccc cccggcttga tcccgacggc gattcgcgcc
    21841 tcggtcgtcg tgctcgtttc gccgtccaac tgccccgccg gcgccggcgt cggccggacg
    21901 tcgatcagcc gcgcccagat cc
//
LOCUS       DAOPCO010000038        20468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2735_length_20468_cov_5.729682, whole genome shotgun sequence.
ACCESSION   DAOPCO010000038 DAOPCO010000000
VERSION     DAOPCO010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 20468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20468
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2735_length_20468_cov_5.729682"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /locus_tag="PLS90_04805"
     CDS             <1..1565
                     /locus_tag="PLS90_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01757.1"
                     /translation="TFGLLLLGLVEIGLGLGVLHLLPARRRYAYAFGGLAAVMLVAVW
                     IGLPAQRGLQPVTTLLDTLVFYKEGPLATVAVMENNLKERTIYVDGVGVAGTDPIMQT
                     DQKSLAHVPMGLLATADSALTVGFGSGGASYSFLLHDRLRRVDCIEISTTVPKAAPEL
                     HAANHGFLEQEQPRYRLIFDDARSYLTHTEQRYDIIATDCTDLRYKSNANLYDLQYFE
                     ACRARLTPDGLVVVWMPLAGLSEDVFKVALRTFHRVFPAMGIFYLPNEPTHYILLIGW
                     QDEIQLSYERFARMLAEDDVRRDLGEIYLDDPVKLLSSFIAGGPRLAEYLAGDVLNTQ
                     NNPVIEFESPKYGYFDKPLIDNLAALMEIYTSPRQFMDTRRMPPEELERLRRYEQALP
                     LIISGHAHYRNLRIEEAAMDYMKAAAITPDDLSLKRLLRFPILSERLRYEPRNSYLYV
                     LLGRVYMLQPDLDKREMAYDLLTQGIELLQEDLAAGEEPEFTSQRLDQARGWHAELER
                     ELQAASAPLSDQ"
     gene            1777..3381
                     /locus_tag="PLS90_04810"
     CDS             1777..3381
                     /locus_tag="PLS90_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01758.1"
                     /translation="MAWQRSRSKYLTAFARFSLLLVLIAAAAGAARAQEPAQTASAPE
                     QPQSDARYQTETGGLNIPPELDPRDPNISKEERIRRHRLFLQERRRQYQERKAAEERQ
                     AQPQNLDELRERAVQVQAGVAGTAPTTGTTPVAETLVTTATLPIRVSEKLDIPHVTFF
                     LTPASQSVRTGEQFATEAALLNPDHLPFDRVRVALRYPSSSFEPVAIHQDTLKPLLKE
                     DPSVQLDSARGTLVYEGLLKVPVNSETFPLITVVWAARQAADSVEIQPLIDEQLFSGA
                     WAGERLVSMSAFGLREALAGATVRIMAAQTAMPAGLKFMQGDLPSLQPILAGFSDQSR
                     LRPPTLWFDQPDSGWLEVGQWLVVDLGLDNPDGMVFDEIRLAGRFDPEVVEIHDADEL
                     NWISRGVNLLDGPFHAQWPWDLQFENTVNNAHGTFYYRMGMTQMRRQPSGVVARILAR
                     VKRRTPAPVFSWVYQRDANPSTPSTGVFLMGENVYLRGTATAAASSPDAAGEALVEAL
                     PRDDAPARLSPPIHEIEKADPAIYRF"
     gene            3444..4196
                     /locus_tag="PLS90_04815"
     CDS             3444..4196
                     /locus_tag="PLS90_04815"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPK01759.1"
                     /translation="MSPRAPHRFHHPGPIGPRAPGERLLLPVSESHHLIDVLRLREGT
                     PIDLFDAAGATYRARFVGRRDGRAEIELLAPLDSASPAGPVVKLAVAMLKRRAMDLLI
                     EKLSELGVDTLQPLHTRRSVIPPRAEDEPGVPERWSRLAIAAAKQCGRPRPLSLPPPM
                     AVEHWLQADRPPADGLVAAFGGNPRPLGEWLRQRPALDRPIWIAIGPEGGWTPAEQAA
                     FESAGYVPVGLGPLTLRAETAAIAAAAACRLM"
     gene            4388..6388
                     /locus_tag="PLS90_04820"
     CDS             4388..6388
                     /locus_tag="PLS90_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01760.1"
                     /translation="MMEPTTASASASESAPPPAGWLNRLLVRWIGGPQSVNQGVLFRL
                     LALFLPHRRRLVLANLLIILASFIGVFSLFAFLPILRVIFNEQPLETEPPPAAAVSPE
                     HQLEDDLLTRLPLVGPLIERGRQRIDTLKQRLQVWEVQANAYLSRNKLEGILIIAGVL
                     LLAALLSALLSYAASLIMARIEEETLGGLSERLYAHCIRQDLGFFHRNPTGQLMTRVY
                     NDVMMLRQPMELLYSTTVREPVTMLFLLIFLLLINPTLTAIVLLMLPLAIIPTIILAR
                     RIRDISREEVMGDAFIMDQMQTSFSGIKLIKSFGSEDFEAQRFGRSNRTMFRRRFRRR
                     MIKELASSTSDIITIIGVVAILVVGALLLFQFGIIGGGAEFMLYLLVLNRFYKPIKKL
                     SALHVGMQRALMSAERIFDVLDTRPRVIDPPDPLPWPEDFDFIRLEDLGFSYGAREEP
                     LFRHLNLWIPRDRVTAFIGRNGSGKTTLAALLCRFYLPTEGRILIGSTPLDRIAEKEL
                     RRRIVMLTQETILFNLTVLENIAYGEDDIDLERAERAARLTRAHEFIEQLPDGYHTVV
                     GERGGRLSGGQARLLTLARVIYRDPDILILDEPEAALDVKNQAVVAEVLREVTRGRTT
                     ILITHKLSSVLHVDKLVRVRRRSTRTIRGKERRTAEALENYI"
     gene            complement(6379..6651)
                     /locus_tag="PLS90_04825"
     CDS             complement(6379..6651)
                     /locus_tag="PLS90_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK01761.1"
                     /translation="MGKTTLLAQLFPDLKRVTFDPSQDLFGARRDPDLFLDSFPPPLV
                     LDEIQFAPELLPALKRRMDENPSPGQYFLSDGAAVAGAVTPRPGQM"
     gene            6777..7934
                     /locus_tag="PLS90_04830"
     CDS             6777..7934
                     /locus_tag="PLS90_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPK01762.1"
                     /translation="MKKRFLFTPGPSNVPPEVLAAMARPIVHHRSPDFDPIFAECSKR
                     LQYVFQTENPVLTFASSGTGAMEAAVTGTLSPGDTMITIEGGKFGQRWGDIGRAYGMN
                     VVTLAVEWGDSVDPARVAQALKDHSDAKAVFATLCETSTATLTDIEALGKVIAPTDAI
                     LAVDAISGLAADVFKTDAWGVDLAVAGSQKALMLPPGLAFLAVSAKARKLIETAKCPS
                     YYFNLKKALKNLADNTTAYTPAVSLIVGLNVALRMIEAEELPNVWCRHSALANALRRG
                     GNGLGLRQFSKAPSNAVVAWHLPEGVSYKDLSLKLRDEHGLTIAGGQDHLKGKIFRAA
                     AMGWADLKDVISFLGALERVLTDLGYAVPQRGGGVAGALEELESYELGFAN"
     gene            complement(8126..9046)
                     /locus_tag="PLS90_04835"
     CDS             complement(8126..9046)
                     /locus_tag="PLS90_04835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK01763.1"
                     /translation="MSQAEPAPLCSVIVCSLNGERVLPACLRSLRSCGAGRTPYEIIV
                     VDNGSTDATPALVRRDFPEARLVQTGRNLGFAGGNNAGMLSARGEILVLLNDDTEVPA
                     DWIDALCAPFARDPAIGAVGCKLLYPDRRTIQHAGGLLYPNGNTGHHGMGEVDNGQWD
                     TPGERAYVTGAALALRRAALAQVGLLDPGYFPIYFEEVDLQTRLARAGWKIHYEPAAW
                     LVHHESQSQGAGSPRFVYRYTKNRIRYLAHFGFVEGLGPAVRAEAAWLRSMARARRLV
                     PVLRAYVYGAVHWPAWRLDRQARRGVPRLP"
     gene            complement(9220..9525)
                     /locus_tag="PLS90_04840"
     CDS             complement(9220..9525)
                     /locus_tag="PLS90_04840"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPK01764.1"
                     /translation="MDPLVDQVAKRIAEAIRPRQIMLFGSRATGRARPDSDLDLLIVY
                     SGGKSKQEVKRSIQRLFPARDFAMDLFVLTPNELETYRHVANTLAREGTERGIACDG"
     gene            9531..10007
                     /locus_tag="PLS90_04845"
     CDS             9531..10007
                     /locus_tag="PLS90_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK01765.1"
                     /translation="MPRRFTIGPPLQSLRGGASVDSMSPWCKSIKKGMEELVEHAKGV
                     PGEFVFLPNPSLHNLFDRILKRAKIPKKDALGRKLTAHSFRHTFATRMAQQVGQNAFI
                     LKELMGHSKISTTEIYCQPEAPRLMMQMPGLAMPEVTGVVEVAWWKKAKTAQLGAA"
     gene            complement(10023..10099)
                     /locus_tag="PLS90_04850"
     tRNA            complement(10023..10099)
                     /locus_tag="PLS90_04850"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10063..10065),aa:Val,seq:cac)
     gene            complement(10344..13121)
                     /locus_tag="PLS90_04855"
     CDS             complement(10344..13121)
                     /locus_tag="PLS90_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01766.1"
                     /translation="MLLLTAFVSLPACYDPNSIEKIALSPDARFAAVVTSDGELGVID
                     LGPAGDKVIYTRMAGRGIDWSGDSRRLLFVEKLPDEPYRLQVVQPGDRKTPARTLLSQ
                     PDWIADPIWLDENQIAFLSTREGTDANLWVFDLRTGELHKRMDRPQEISQAWSVPGGG
                     QVLFQCLERGLPQLWLWEASRADEPRLLPRAEGDVSLEQRLVAIAPDGRGLAVLPRGT
                     GPRPLLYYDLRMERPIASHTIVGPVESMAVLGDGRIALAQNLSLTDGSLRPVLDLPRL
                     LTTEAENAIILWNPDPGLFGKEIELTGWANLPLTQLERFERQGALMAVNTNVLMLADD
                     IESIAEGNLFIRRTEDAIFMILAQARNGHLEMAREALRRLWDLPGANGPERAYLLAMA
                     RAQVERMSGDWDEALRWIEEAIAAEPDSYPMLETAWMEYLAVAWFGAGDTQRAGWALR
                     QMPAEMRSTPLAVWVSALIDAWPGEEARAWTGIIRDLRAHDLPAAARGAQRAIHRDAP
                     TTLALTGLELMLNGEIEPAATAVGVQWLTTERLMERLEFQQAMLTAGRLEETGVGLPP
                     SDLRTLVLSQWLRRGDVRAAQQLVQEDLSATATTNMDYSEILLSFLEPEEQEPWMERA
                     VSDVLLAEPVAPALRRLFSDPAGRLMTLQARIKASLIAGDMAPAVEALEEAVLLISQP
                     EARQAADADVLFRLTLYQAKVNERQGEWEAAAEGYRAALRWMARDARAWQPTGSELAL
                     SIGLLAHARDDRELLQSYLRIQRGLGDPVINPTHDAATLRAALDNLRTLRQFSTDAAW
                     LAPYLAYCEAIIWSLLRYPMQALESIEEVKRLDPDPTLLQRALLEEAAIREHLGQFAL
                     AARIYERIYRMELPIAVRGPALLSGIVAEISAGLTDLPGDRLDELAAEVPIPPNWLRW
                     LRLQLAADLGG"
     gene            13640..14566
                     /locus_tag="PLS90_04860"
     CDS             13640..14566
                     /locus_tag="PLS90_04860"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HPK01767.1"
                     /translation="MNVFNSLAPIFLIIGLGALLSRIGCLTPPLVAGANAFAYWIALP
                     ALLFRETATAQVNLAGHWDAFAVLLGGMLVCVAAGYLAAWIIRLPGQDAGTLVQAAFR
                     GNLAYIGLAVVILSAENGASDPAALSVLMLAGIVPFYNVVAVIVLLASQHRFEWGAVR
                     RVLRQLASNPLIIASVAGALVMLSSWSLPAVVDRTLRPIAQTGLPLALLGIGATLHSE
                     RIRGGWLPVGTAGLIKVALAPAFGILAGGAIGLTPDELRIVLIYLACPTAAASYVMAD
                     QLGGNRILAAGCVVFSTLASFVSLSVILGLVQ"
     gene            14563..16179
                     /locus_tag="PLS90_04865"
     CDS             14563..16179
                     /locus_tag="PLS90_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01768.1"
                     /translation="MSGAADASPARRRSRFLPLGRQRLLWVALLAGAALSLGIYHHLP
                     WLNGPPQWKWPWVHERILGRALAQSAARFVLGAIVLGGALLLVRRRLRRPAARRAAGL
                     ATLILWGLYAQWTLYAVATPLGPLHSIRIVRDEIAFGYFGVAAALGDRLPPLPEFLRR
                     YPQYMPALPPHASTKPPGPVVACRALRRLFETAPRLTERIWRLAVRLDPRLELAETSG
                     SRADCAAAFTLAQLLMLAGLSAAWPLHALVRRSGGLPWREPAAWCAAWLWVHYPALAI
                     FHPQFDQLYPLLALGALLCLARAIDPRQPAAGLPPALGLGLLLTLGLLLSFTMLFLIP
                     LVLIPVPLAAARRARSWRLRAIADQLGWRRPWGRRVGRALAAAGFIPVAVLFILRASL
                     GFDWIDTFLTALRHQHGTLLRGIERSYATWLGWNLYDFLLFAGGTVATFGLVFLGGAV
                     RRGWRRPRRLLPIWFAFPFVLLLLDLSGLTPGETGRIWLFLAPGLVWAAAIELARRAR
                     AGWPTALFLVLLAQWIFLLVLRTRMHYIGW"
     gene            16529..17353
                     /locus_tag="PLS90_04870"
     CDS             16529..17353
                     /locus_tag="PLS90_04870"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPK01769.1"
                     /translation="MKSLVKSIGAASRLARLLALGAVAAWVASPAGAQPPAPPDDLAA
                     PAFPEDAGDALPGGGFEPGFGPAMGPGMGPGPGMGPGFGIGRGFGAPGMGRGMGPGLG
                     RGPGGGRASCWAAQTLLQPDVQATLGLTQQQADRIEQTDYDTQSRLIAKRAELEQAQL
                     ELRRLCDDPDIDRTALMTQIDRLGALRTELHKLNAGRLLDVRETLTPEQRQQAREVIG
                     QRALVQRSQQRPFIGGRRGAMRGGPGPGGPGMGWNRPGMPGGQGQQPPLAPAPPAQ"
     gene            complement(17555..19294)
                     /locus_tag="PLS90_04875"
     CDS             complement(17555..19294)
                     /locus_tag="PLS90_04875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPK01770.1"
                     /translation="MAETSAIQAKRGEEFPRDFTRHVTDLGDWSQLEPLFDDLASQIR
                     MIELPEHLRQWLRRQSELDAVIDEEYARRYIAMTCQTDDKEREAAYLHYVENILPKIK
                     PWHDKIARLYLECPAREALDRDWLRVHDRALQNQVELYREENIPLQTEDQKLGNEYQK
                     ISGAMTVMWEGEEKTLQQMAVYTEDADRGVRERAWRLVAERRLADRDAINAVFAKLID
                     LRTRMARNAGFANFRDYQHRAYNRFDYTPEDCLTFHRAIEQEVVPRLAAQRRVRREKL
                     KLERLRPWDLSVDVDGKAPLKPFSAAEDLAQRCGAIFADLGPTLAAQFQKMRDLGLLD
                     LESRKGKAPGGYQYTLEEVRLPFIFMNAAGTHRDVETLLHEGGHAFHAFAARDQEILT
                     YRSAPIEFCEVASMAMELFGMKHIERFYPDEGDARRARRRHLEQILSIFPWIATVDAF
                     QHWIYLNPEHTVAEREAEWLRLEERFDPEIDWSGLDAELRNQWQRQLHIFLHPFYYVE
                     YGIAQLGALQIWLKFRREPRKALEDYLNGLALGGSRPLPELFAAAGIRFDFSAATLAP
                     VMQAIEEELASLN"
     gene            complement(19437..20399)
                     /locus_tag="PLS90_04880"
     CDS             complement(19437..20399)
                     /locus_tag="PLS90_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPK01771.1"
                     /translation="MQEALSAKAAHDDLRRLEQNIELVIRGKHQSVRMAITGLLAGGH
                     VLFEDVPGVGKTTLAQCLAMSINVTFQRIQGTSDLLPSDILGVSVWDNDKKAFVFRAG
                     PIFANIVLVDEINRTTPKTQSALLEAMNTAQVSIDRNTYDLAQPFMVIATQNPFDSHG
                     TFPLPKSQLDRFMMRLHLGYPDAEYEREILRNTRSTGDLTMVKPVLSSERIRAMQEGV
                     RRVRVDDSLVDYIVNLINATRHSPLIELGASTRAALALRNCAKANAYMDGRDYAIPDD
                     VKAVAVSVLAHRLGIIRTFEETAESLGEGSSAVRAILEETPVPI"
BASE COUNT         3205 a         7544 c         6336 g         3383 t
ORIGIN      
        1 tgaccttcgg cctgctgctg ctgggcctgg tcgaaatcgg cctggggctg ggcgtgctgc
       61 atctgctgcc ggcccgccgg cgctacgcct atgccttcgg cggcctggcg gcggtcatgc
      121 tcgtcgctgt ctggatcggc ctgcccgccc agcgggggct gcagcccgtg acgaccctgc
      181 tcgacactct ggtgttctac aaggaaggcc cgctggcgac cgtcgccgtg atggagaaca
      241 acctgaagga gcggacgatc tacgtcgatg gcgtcggcgt cgccggcacc gatccgatca
      301 tgcagaccga tcagaagtcg ctggcgcacg ttccgatggg cctgctggcc acggccgaca
      361 gcgccctgac cgtcggcttc ggcagcggcg gcgcgtcata ttcgttcctg ctgcacgacc
      421 gcctgcgccg cgtcgattgc atcgagatct cgacgaccgt tcccaaggcg gcccccgaac
      481 tccacgccgc caaccacggc ttcctggaac aggaacagcc gcgctaccgg ctgattttcg
      541 acgacgcccg ctcgtacctg actcacaccg aacagcgcta cgacatcatc gccaccgact
      601 gcaccgacct gcgctacaag agcaacgcca acctgtatga cctgcaatac ttcgaggcct
      661 gccgcgcgcg cctcaccccc gacggcctcg tcgtcgtctg gatgccgctg gccggcctca
      721 gcgaggacgt cttcaaagtc gcgctgcgca cgttccaccg cgtcttcccg gcgatgggca
      781 tcttctacct ccccaacgag ccgacgcatt acatcctgct catcggctgg caggacgaaa
      841 tccagctcag ctacgagcgc ttcgcgcgca tgctcgccga ggatgacgtg cggcgcgatc
      901 tgggcgagat ctacctggac gacccggtca agctcctctc ctcgttcatc gccggcggtc
      961 cccggctggc cgagtacctc gcgggcgacg tcctcaacac ccagaacaat ccggtcatcg
     1021 aattcgagtc gcccaaatac ggctacttcg acaagccgct gatcgacaac ctggccgcgc
     1081 tgatggaaat ctacacctcg ccgcgccagt tcatggatac ccgccgcatg ccgcccgaag
     1141 agctcgagcg cctgcgccgc tacgaacagg cgttgccgct gatcatctcc ggtcacgccc
     1201 actaccgcaa tctgcgcatc gaggaagcgg ccatggatta catgaaggcc gcggcgatca
     1261 cccccgacga cctcagcctg aaacgcctgc tgcgcttccc catcctgagc gagcggctgc
     1321 ggtacgagcc gcgcaattcc tatctgtatg tcctgctcgg acgggtctac atgctccagc
     1381 ccgacctcga caagcgcgag atggcgtacg atctgctcac ccaggggatc gaattgctgc
     1441 aggaggatct cgccgccggc gaggagccgg agtttaccag ccagcgcctg gaccaggcgc
     1501 gcggctggca cgccgaactc gagcgcgagt tgcaggccgc atccgccccc ctgagcgacc
     1561 aataaaccgg gcttttgcgc gcggcgccag agccacgcaa tgcatcggcg ctgctttcct
     1621 cacaacaccc gaaccgctat ttcacggcaa cctgccgcgc atccctatct gtcccctcca
     1681 atctgcccgc cgtttccggc cccgggcttt cctttgccat ctggcgcggc gattgcatac
     1741 actggatcat ctcccgcggg gaggggtggc ggattcatgg cctggcaacg ctcccgctca
     1801 aagtacctaa cggctttcgc gcgattcagc ctgctgctcg tcctgatcgc cgcagcggcc
     1861 ggggccgccc gcgcgcagga accggcgcaa accgcgagcg ccccggaaca gccccagtcc
     1921 gacgcccgct atcagaccga aaccggcggg ctcaacatcc cgcccgagct cgatccgcgc
     1981 gacccgaaca tcagcaagga agagcggatc cgccgccacc gcctgtttct gcaggaacgc
     2041 cgccgccagt atcaggagcg caaagcggcc gaggagcgcc aggcgcagcc gcagaatctc
     2101 gatgaattgc gcgaacgcgc cgttcaggtc caggccggcg tggccggcac ggcccccacc
     2161 accggcacca cgccggtcgc cgaaacgctc gtcaccaccg ccacgctgcc gatccgcgtc
     2221 agcgaaaaac tcgatatccc gcacgtcacg ttcttcctga cgcccgccag ccagagcgtc
     2281 cgcacgggcg aacagttcgc caccgaggcg gcgctgctca atcccgacca cctgccgttc
     2341 gaccgcgtgc gcgtcgctct gcgctatccc tcctccagtt tcgagcccgt ggcgatccac
     2401 caggatacgc tcaagccgct gctcaaggag gacccgagcg tccagctcga ttccgcgcgg
     2461 ggaacgctcg tctatgaagg gctcctcaag gtaccggtca attcggagac attcccgctg
     2521 atcacggtcg tctgggccgc ccggcaggcc gccgattcgg tcgaaattca gccgttgatc
     2581 gacgagcagc ttttctcggg cgcctgggcc ggcgagcgcc tggtctcgat gtcggccttc
     2641 ggcctgcgcg aagcgctggc gggagccacc gtccggatca tggcggccca gaccgcgatg
     2701 cccgcgggcc tcaagttcat gcagggcgat ctgccgtcgc tccagcccat cctcgccggc
     2761 ttttccgatc agtcccgtct gcggccgccg accctctggt tcgaccagcc cgacagcggc
     2821 tggctcgagg ttggccagtg gcttgtcgtt gatctgggtc tcgacaaccc cgacggcatg
     2881 gtcttcgacg agattcgcct cgcgggccgg ttcgaccccg aggtggttga aatccacgac
     2941 gccgacgaac tgaactggat cagccgcggc gtcaatctgc tcgatggccc gttccatgcc
     3001 cagtggccgt gggacctgca atttgaaaac accgtgaaca atgcccatgg gacgttctat
     3061 taccgcatgg ggatgacgca gatgcggcgg caaccctcgg gcgtggtcgc gcggattctg
     3121 gcccgcgtca aacgccggac gcccgccccg gttttctcct gggtttacca gcgcgacgcg
     3181 aacccctcga cgcccagcac cggcgtgttc ctgatgggcg aaaacgttta tctgcgcggg
     3241 accgcgacgg ccgctgcaag ctccccggat gccgccggcg aggcgctcgt cgaggcgctt
     3301 ccgcgcgacg acgcgccggc ccgcctgagc ccgccgatcc atgagatcga gaaagccgac
     3361 cccgcgatct accgtttctg acggcgggcg agagcaagcg cccatgacgc gcgccgcgcc
     3421 ccgccgccaa tgaagtcccc gcgatgagcc cacgcgcccc ccatcggttc caccaccccg
     3481 ggccaattgg cccgcgcgcg cccggcgagc ggctcctgct gccggtaagc gaatcgcacc
     3541 acctgatcga tgtcctgcgg ctgcgcgagg ggaccccgat cgatctgttc gatgcggcgg
     3601 gcgccaccta tcgcgcccgc ttcgtcggcc ggcgcgacgg gcgcgccgaa atcgagctgc
     3661 tggcgccgct cgattcggct tctccagcgg gcccggtcgt caagctggcg gtcgccatgc
     3721 tcaagcgccg ggcaatggac ctgctgattg aaaaactctc ggaactgggc gtcgatacgc
     3781 tgcagccgct ccacacgcgc cgcagcgtca tcccgccgcg ggccgaggac gagccgggcg
     3841 tccccgagcg ctggagtcgg ctcgcaatcg ccgccgcgaa acaatgcggc cggccccgtc
     3901 cgctgtcgct gccgccccca atggccgtcg aacactggct gcaagctgac cgcccccccg
     3961 ccgacgggct cgtggccgcc ttcgggggga acccccgtcc gctgggcgag tggctgcgcc
     4021 aacgcccggc gctggaccgg ccgatctgga tcgcgatcgg ccccgagggg gggtggacgc
     4081 ccgcggaaca agcggccttt gagtcggccg gctacgtgcc ggtcggcctg gggccgctga
     4141 cgctgcgggc cgagacggcg gcgatcgctg cggccgcggc ctgccggctg atgtaacctc
     4201 agacgacgcc aaccgcaaac cgcccgccgc gcccatcgga caggttgccc ttttttgttt
     4261 gaacggcgtg gggcgctgtg ctcagttgaa gtcagggccg gcccccggcc ggcacgtctg
     4321 cgacccgccc ccggcgcgga cctagcatgg ccccgctgag cccaccgggg acgaatccct
     4381 cccgcccatg atggaaccca ctaccgcgtc cgcgtccgcg tcggagtcgg caccgcctcc
     4441 cgccggctgg ctcaatcggc tgctggtgcg ctggatcggc ggcccccagt cggtcaacca
     4501 gggcgtcctg ttccggttgc tcgccctgtt tctgccccac cggcgtcggc tcgtcctggc
     4561 caacctcctg atcattctgg ccagcttcat cggcgtcttc tcgctgttcg ccttcctgcc
     4621 catcctgcgc gtgatcttca acgaacagcc gcttgagacc gaaccgcccc ccgccgccgc
     4681 cgtctcgccc gagcatcaac tggaggacga tctgctgacg cgcctcccgc ttgtcggccc
     4741 gctgatcgaa cgcggtcgcc agcgcatcga cacgctcaag cagcggctcc aggtctggga
     4801 ggtccaggcc aacgcctacc tgagccgcaa caagctggaa gggatcctga tcatcgccgg
     4861 cgtcctgctg ctcgccgcac tgctcagcgc gctgctctcc tacgccgcca gcctcatcat
     4921 ggcgcggatc gaggaggaaa cgctcggcgg cctctccgaa cgcctctacg cccactgcat
     4981 ccgccaggac ctggggttct tccaccgcaa tccgaccggc cagctcatga cgcgcgtcta
     5041 caacgacgtg atgatgctcc gccagccgat ggaactgctc tacagcacca cggtccgcga
     5101 gccggtcacg atgctgttcc tgctgatctt cctgctcctg atcaacccga cgctgacggc
     5161 gatcgtcctg ctgatgctcc cgctggcgat catcccgacg atcatcctgg cgcggcggat
     5221 ccgcgacatc tcgcgcgagg aggtcatggg cgacgccttc atcatggacc agatgcagac
     5281 gtcgttcagc ggcatcaagc tgatcaagtc gttcggcagc gaggatttcg aagcgcaacg
     5341 cttcggtcgc tccaaccgca ccatgttccg ccgccggttc cgccgccgga tgatcaagga
     5401 gctggcctcc tccacctccg atatcatcac gatcatcggt gtcgtcgcca tcctggtcgt
     5461 cggggcgctg ctcctgtttc aattcggcat catcggcggc ggcgccgaat tcatgctcta
     5521 tctcctcgtg ctcaaccggt tctacaagcc gatcaagaaa ctgtcggccc tgcacgtcgg
     5581 gatgcagcgc gcgctgatga gcgccgaacg gatcttcgac gtcctcgaca cccggccccg
     5641 cgtcatcgat ccgcccgatc ccctcccctg gccggaagac ttcgatttca tccggctgga
     5701 agacctgggc ttctcctacg gcgccaggga ggaaccgctc ttccgccacc tgaacctgtg
     5761 gatcccgcgc gaccgggtca ccgccttcat cggccgcaat ggctcgggca aaaccaccct
     5821 cgccgccctg ctctgccggt tctacctccc caccgaaggc cgaatcctca tcgggtcgac
     5881 gccgctcgac cggatcgccg aaaaggagtt gcgccggcgg atcgtcatgc tgacccagga
     5941 gacgatcctg ttcaacctga ccgtgctgga gaacatcgcc tacggcgagg acgacatcga
     6001 cctggagcgg gcggaacggg ccgcccggct gacgcgcgcc cacgaattca tcgagcagtt
     6061 gcccgacggc taccacaccg tcgtcggcga gcgcggcggg cgcctctcgg gcggccaggc
     6121 gcgcctcctc acgctcgcgc gcgtcatcta ccgcgacccc gacatcctga tcctcgacga
     6181 gccggaggcg gcgctcgacg tgaaaaacca ggcggtcgtc gccgaggtcc tgcgcgaagt
     6241 gacgcgcggg cgcacgacga tcctgatcac gcacaaactc tcttcggtcc tccacgtgga
     6301 caaactggtc cgcgtccgcc gccgcagcac ccgcacgatc cgcggcaagg aacgccgcac
     6361 cgccgaagcc ctcgaaaatt acatctgacc cggccggggc gtcacagcgc ctgccacggc
     6421 agcgccgtcg gaaaggaagt actggcccgg cgacgggttc tcatccatcc tccgtttgag
     6481 ggcgggcagc agctcgggtg caaattggat ctcgtccagg accagcggag ggggaaaact
     6541 gtccagaaac aggtccgggt cccggcgcgc gccgaacaga tcctggctcg ggtcaaacgt
     6601 gacgcgcttc aggtcgggaa acagctgggc cagcagcgtg gttttgccca cctgcgcccg
     6661 cccacggcct tgtcttgcct ttcagattgt gatagctgtt atgggtttac cggccggatc
     6721 gccatggccg ctgactccat cgcccgcccc ccgattcaac taaaggcgcg cccatcatga
     6781 aaaaacgctt ccttttcacc cccgggccct ccaacgtccc gcccgaagtc cttgcggcca
     6841 tggcccgccc catcgtccat catcgctcgc ccgatttcga cccgatcttc gccgagtgct
     6901 ccaagcgcct gcaatacgtc ttccagaccg aaaaccccgt cctcaccttc gccagttccg
     6961 gaaccggcgc gatggaagcc gccgtcacgg ggaccctctc gcccggcgac acgatgatca
     7021 ccatcgaggg gggcaagttc ggccagcgct ggggcgacat cggccgcgcc tacggcatga
     7081 atgtcgtcac cctggccgtt gaatggggcg actccgtcga ccccgcgcgc gtcgcccagg
     7141 cgctcaagga ccactccgac gccaaggcgg tcttcgccac gctgtgtgaa acctcaaccg
     7201 ccaccctgac cgacatcgaa gcgctgggca aggtcatcgc tccgaccgac gccatcctgg
     7261 ccgtcgacgc catcagcggc ctggcggccg atgtcttcaa aaccgatgcc tggggcgtcg
     7321 acctcgccgt cgccggctcg cagaaggcgc tcatgctccc gcccggcctc gccttcctcg
     7381 cggtcagtgc caaggcccgc aagctgatcg aaaccgccaa atgcccgagc tattacttca
     7441 acctcaagaa ggcgctcaag aatctggccg acaacaccac cgcctacacc cccgccgttt
     7501 cgctcatcgt cggcctcaac gtcgccctgc ggatgattga agccgaggag ctgcccaacg
     7561 tctggtgccg acactcggcg ctggccaacg cgctgcgccg cggcggcaac ggcctcggcc
     7621 tgcgccagtt cagcaaggcc ccgtccaatg ccgtcgtcgc ctggcatctg cccgaagggg
     7681 tcagctacaa ggacctgtcg ctcaaactgc gcgacgagca cggcctgacg atcgccggcg
     7741 gccaggacca cctcaagggc aaaatcttcc gcgccgccgc gatgggctgg gccgacctga
     7801 aggacgttat cagcttcctc ggcgccctcg agcgcgtcct gaccgacctg ggctacgccg
     7861 tgccccagcg cggcggcggc gtcgccggcg ccctcgagga actggaaagc tacgagctgg
     7921 gcttcgcgaa ttaagccccc ctgcgtagcg attcagccaa ttccttggac ccttcacgaa
     7981 atcgccatcg ggatcggtag cgctatcgcc tccgccggtg tgggaccggc gccctcgccg
     8041 gtcaccccgc atcccgctgg gatgccaggt tcatcgcgcg atacgtcagc ttgaggccaa
     8101 aaccaagccg gctgcccgcg cccccttacg gcaggcgcgg cacgccgcgg cgcgcctgcc
     8161 ggtcgagccg ccacgccggc caatggaccg ccccataaac ataggcccgc agcacgggga
     8221 ccagccgccg ggcgcgcgcc atcgaacgca gccaggccgc ctcggcgcgg acagccggcc
     8281 ccaacccctc cacgaacccg aagtgcgcca ggtagcggat ccggttcttc gtgtagcgat
     8341 agacgaaccg cggactgccc gccccctgcg actgcgactc gtgatggacg agccacgccg
     8401 ccggctcgta gtggatcttc cagccggcgc gggcgaggcg cgtctgcaga tcgacctcct
     8461 cgaaataaat gggaaagtag cccggatcga gcagcccgac ctgggcgagt gccgcgcgcc
     8521 gcagcgccag cgccgcgccg gtcacatacg cccgttcgcc cggcgtgtcc cactggccgt
     8581 tgtcgacctc ccccatccca tggtggccgg tgttgccgtt gggatagagc aggccgcccg
     8641 cgtgctggat cgtgcggcgg tcggggtaga gcagcttgca gccgaccgcg ccgatggccg
     8701 ggtcgcgcgc gaacggggcg cacagcgcgt cgatccagtc ggccgggacc tcggtgtcgt
     8761 cgttgagcag gaccaggatc tcgccgcggg cggacagcat cccggcgttg ttcccgccgg
     8821 cgaaccccag attgcgcccg gtctggacca gccgcgcttc gggaaagtcg cgccggacca
     8881 gcgcgggcgt ggcgtcggtc gacccgttat cgacgacgat gatctcgtac ggcgtccgcc
     8941 ccgcgccgca cgaccgcagc gaccgcagac acgccggcaa tacccgctcg ccgttgagcg
     9001 agcagacaat gacgctgcac aggggcgcgg gttcagcttg gctcatcgtg agacttgcag
     9061 gtgcgagagg atggtctgca acagcggaca gcttcccaat attcgccgcc atcagaagga
     9121 ttggttacgg tgcgccccgt tggttacggt tcgcccagat cgtaagagag ttcgttacgg
     9181 gccttttcca gccagcgggg aacaaccttc cgtttcgcct catccatcgc aggcgatccc
     9241 ccgctcggtc ccctcgcgcg ccaacgtgtt ggcgacgtga cggtaggtct ccaattcatt
     9301 cggagtcagc acgaacaggt ccattgcgaa atcgcgagca ggaaagagcc gttgaatcga
     9361 ccgtttgact tcctgtttgc tcttaccgcc cgagtagacg atcagcagat ccaggtcgct
     9421 gtcgggacgc gctctccctg tcgcccgaga gccaaacagc atgatctgcc tggggcggat
     9481 cgcttcagcg atccgtttag cgacctggtc gaccagcggg tccatccgac atgcctcgtc
     9541 gattcacgat cggcccgccg ctccagagcc tgcgcggcgg tgcttccgta gacagcatgt
     9601 ccccgtggtg caaatcgatc aagaagggaa tggaagagct cgtcgagcat gccaagggag
     9661 tgcctgggga gtttgtcttt ctccccaatc caagcctgca caacctcttt gaccggatcc
     9721 tcaagcgggc gaagataccg aagaaggatg ccctgggacg aaagctgacg gcgcattcct
     9781 tccggcacac cttcgccacg cggatggctc agcaagtcgg acagaacgct ttcatcctga
     9841 aggagctgat gggacacagc aagatctcaa caacagagat ctactgccag ccggaagcgc
     9901 ccagactgat gatgcagatg cccggccttg ccatgccaga ggtcacgggg gtggtggaag
     9961 ttgcctggtg gaagaaagca aaaaccgccc aactcggggc ggcgtaaggc caatcgattc
    10021 aatggtgggt ggtacaggat tcgaacctgt gacctctggc atgtgaggcc agcgctctaa
    10081 ccaactgagc taaccaccca cgcgaagggg agaagccgac ggcgagggcc acgcttgcgg
    10141 gtccgcgaga cccccgcgcc cgacgccgct cggaccctga aactaccgca aaaagccgcg
    10201 aatttgcaag cgcaatggct ggcgccggag cgctcagaat cggtatcggt atcggtatca
    10261 gtatcggtat cggactcgct atccgattcc gctatcgcaa tcgggaccag actcgaacaa
    10321 ccgcccaccc ggccgcgccc cgctcaccct cccaggtcgg cggccagttg cagccgcagc
    10381 cagcgcagcc agttcggcgg gatcggcacc tccgccgcca gttcgtccag ccggtcgccc
    10441 ggcagatcgg tcaggccggc gctgatctcg gcgacgatcc cgctcagcag cgccggcccc
    10501 cgcacggcga tcggcagctc catccgatag atccgctcat aaatccgcgc cgccagcgcg
    10561 aactggccca ggtgctcgcg gatcgccgct tcctccagca gcgcccgctg cagcagcgtc
    10621 ggatccggat cgagccgctt cacctcctca atcgactcga gcgcctgcat cggatagcgc
    10681 agcagcgacc agattatcgc ctcgcagtag gccagatacg gcgccagcca ggcggcgtcg
    10741 gtgctgaact ggcgcagcgt gcgcaggttg tcgagcgccg cgcgcagcgt cgccgcgtcg
    10801 tgcgtcgggt tgatcaccgg gtcgcccagg ccgcgctgga tgcgcaggta gctctgcagc
    10861 agttcgcggt cgtcgcgcgc gtgcgccagc aggccgatcg acagcgccag ctcgctgccg
    10921 gtgggttgcc aggcgcgcgc gtcgcgcgcc atccagcgca gcgccgcccg gtagccttcg
    10981 gccgccgcct cccattcccc ctggcgctcg ttcaccttgg cctggtagag cgtcagccgg
    11041 aacagcacgt cggcgtcggc cgcctggcgc gcctcgggtt gcgagatgag caggaccgcc
    11101 tcctccagcg cctcgaccgc cggggccatg tcgccggcga tcagcgacgc cttgatccgc
    11161 gcctgcagcg tcatcagccg cccggccggg tcgctgaaca ggcggcgcag cgccggcgcc
    11221 accggctcgg ccagcagcac gtcgctgacg gcccgctcca tccacggctc ctgctcctcc
    11281 ggctccagga agctgagcag aatttccgag taatccatgt tggtcgtggc cgtcgcgctc
    11341 agatcctcct gcactagttg ctgcgcggcg cgcacgtcgc cccgccgcag ccattggctc
    11401 agcaccagcg tccgcaggtc cgagggaggc aggcccacgc cggtctcttc cagccggccg
    11461 gccgtcagca tcgcctgctg gaattcgagc cgttccatca ggcgttcggt cgtgagccat
    11521 tgcacgccga cggcggtggc ggccggttca atctcgccgt tgagcatcag ttccaggccg
    11581 gtcagcgcca gcgtggtcgg cgcgtcgcgg tggatcgcgc gctgcgcgcc gcgcgccgcc
    11641 gccggcaggt cgtgcgcgcg caggtcgcgg atgatcccgg tccacgcccg cgcctcctcg
    11701 cccggccagg cgtcgatcag cgccgagacc cagacggcca gcggcgtcga gcgcatttcc
    11761 gccggcatct ggcgcagcgc ccagccggcg cgctgcgtgt cgcccgcccc gaaccacgcc
    11821 accgccagat actccatcca cgccgtctcg agcatcggat aggagtccgg ttcggcggcg
    11881 atcgcctcct caatccagcg cagcgcttca tcccagtccc ccgacatccg ctccacctgc
    11941 gcccgcgcca tcgccagcag gtaggcgcgc tccgggccat tggcccccgg caaatcccac
    12001 aaccggcgca gcgcttcgcg cgccatctcc aggtgcccgt tgcgcgcctg ggcgaggatc
    12061 atgaagatcg catcctccgt ccggcggatg aacagattgc cctcggcgat cgattcgatg
    12121 tcgtcggcca gcatcagcac gttcgtgttg acggccatca gcgcgccctg gcgctcgaac
    12181 cgctccagct gcgtcagcgg cagattggcc cagccggtca gttcgatctc cttcccgaac
    12241 agcccggggt ccggattcca caggatgatc gcgttctccg cctcggtcgt cagcaaccgc
    12301 ggcaggtcca gcaccggccg cagcgagccg tcggtcaggc tcaggttctg cgccagggcg
    12361 atgcggccgt cgcccaggac cgccatgctt tcgaccgggc cgacaatcgt gtggctcgcg
    12421 atcggccgtt ccatccgcag atcgtagtag agcaacgggc gcggtcccgt cccccgcggc
    12481 agcaccgcca gcccgcggcc gtcgggcgcg atcgccacca gccgctgttc gagcgaaacg
    12541 tctccctcgg cgcgcggcag cagccgcggc tcgtcggcgc ggctcgcctc ccacagccat
    12601 agttgcggca gaccccgctc cagacactga aacagaacct gtccgccgcc gggcaccgac
    12661 catgcctggc tgatctcctg cggccgatcc atccgcttgt gaagctcgcc ggtgcgcagg
    12721 tcgaacaccc acagattggc gtcggttcct tccctcgtgc tcaaaaaggc aatctggttt
    12781 tcgtcgagcc agatcggatc ggcgatccag tccggctggc tcagcagcgt cctggccggc
    12841 gtcttccggt cgcccggctg caccacctgc agccgatagg gttcgtcggg cagcttctcg
    12901 acgaacagca gccgccggct gtcgcccgac cagtcgatgc cgcgccccgc catccgcgtg
    12961 tagatgacct tgtcccccgc cggccccagg tcgatcaccc ccagctcgcc gtcggacgtc
    13021 acgaccgccg cgaaccgggc gtcgggcgac agcgcgatct tctcgatcga gttggggtcg
    13081 tagcaggccg gcagagaaac gaacgcggtt agcaacagca ccgcacccca ccgcccacgg
    13141 agcaaccggc gcgcgatggc cggaaaacca aaggccaaat ccgagagggt gcgggccggt
    13201 tcggcgacgg gggatgcagg catgcgaaaa cgccggttgc gaggtgcacc aggattgcgg
    13261 ggaggtcgcg gcgaacggcg gaccggagtc cgcctcagtc gccattcaat cacgcgccac
    13321 ggagcctgtc aaacccggcc ccggaagtcc ctcaccctcc cgcgaaaagt cgtctctcgt
    13381 cgccgtccct cgccctcgtg atcgtcctca acggcccgcc ccaggattca cccgcggcca
    13441 tttgtccctg gctctccgat cccctcttcc tgtgccggtg atcccttctg tcctttacgt
    13501 cccctatgtc ctttccatct ccaccgcccc acgacgacag gcgacaaccg gcgcgagccg
    13561 tatttcgcag cccccgaata cccatggcga ttcccgcgcg gaacgcttat gactattcct
    13621 tttgacccga ggcgccccga tgaacgtctt caactcgctt gccccgatct tcctgatcat
    13681 cggcctcggc gcgctgctca gccgcatcgg gtgcctgacg ccgccgctgg tcgccggcgc
    13741 caacgccttc gcctattgga tcgcgctgcc cgccttgctc tttcgcgaga ccgccacggc
    13801 ccaggtcaac ctggccggcc actgggacgc cttcgccgtc ctgctcgggg gcatgctggt
    13861 ctgcgtcgcg gccggctatc tggcggcatg gatcatccgc ctgccggggc aggatgccgg
    13921 cacgctggtc caggcggcgt ttcgcggcaa tctggcttac atcggcctgg cggtcgtgat
    13981 cctcagcgcc gaaaacggcg cctccgaccc ggcggcgctc agcgtcctga tgcttgcggg
    14041 catcgtcccg ttctacaacg ttgtcgcggt catcgtcctg ctcgccagcc agcaccggtt
    14101 tgagtggggg gccgtccgcc gcgtgctgcg ccaactggcg agcaacccgc tgatcatcgc
    14161 cagcgtcgcc ggcgcgctcg tcatgctgtc gagctggagc ctgcccgccg tggtcgaccg
    14221 cacgctgcgg ccgatcgcgc agaccggcct gccgctggcc ctgctgggga tcggcgcgac
    14281 gctgcacagc gagcgcatcc gcggcggctg gctgccggtc ggaaccgccg ggctgatcaa
    14341 ggtcgccctc gcccccgcct tcggcatcct ggcgggcggg gccatcggcc tgacccccga
    14401 cgaactgcgg atcgtgctga tctatctggc ctgccccacc gccgccgcca gctacgtcat
    14461 ggccgaccag ttgggcggca atcgcatcct cgccgccggc tgcgtcgtct tcagcacgct
    14521 ggcttcgttc gtctcgctca gcgtgatcct ggggctcgtg caatgagcgg cgcggcggac
    14581 gcatcccccg cgcgccgccg gagcaggttc ctcccgctcg gccgccagcg gctgctctgg
    14641 gtcgcgctgc tcgccggcgc cgccctctcc ctgggcattt accatcatct gccctggctg
    14701 aacggcccgc cgcagtggaa atggccgtgg gtccacgagc ggatcctcgg gcgcgcgctg
    14761 gcgcaatcgg cggcccgctt cgtcctgggc gcgatcgtcc tcggcggcgc cctgctgctc
    14821 gtccggcgcc ggctgcggcg ccccgcggcc cgccgcgccg ccggactcgc cacgctgatt
    14881 ctctggggcc tttatgcgca gtggaccctc tacgccgtgg ccacgccgct ggggccgctt
    14941 cattcgatcc gcatcgtccg cgatgagatc gcgttcggct atttcggcgt cgcagccgcg
    15001 ctcggcgacc ggctcccgcc gctgcccgaa ttcctccgcc gctacccgca gtacatgccg
    15061 gccctccccc cgcacgcctc caccaaaccg cccgggccgg ttgtcgcctg ccgcgcgttg
    15121 cggcgcctgt tcgagaccgc gccgcgcctg accgaacgaa tctggcggct ggccgtgcgc
    15181 ctcgacccgc gcctcgaact ggccgaaact tccggctcgc gcgccgattg cgcggccgcg
    15241 ttcaccctgg cgcagctcct catgttggcc ggcctgagcg cggcctggcc gctccacgcg
    15301 ctggtccggc gctccggggg cttgccctgg cgcgaacccg ccgcctggtg cgccgcctgg
    15361 ctctgggtgc actacccggc gctcgccatc ttccacccgc agttcgatca gctctacccg
    15421 ctcctcgcgc tgggggcgct gctgtgcctg gcgcgcgcga tcgatccccg gcaaccggcg
    15481 gccggtctgc cgccggccct ggggttgggc ctgctgctga cgctgggcct gctgctgagc
    15541 ttcacgatgc tgttcctgat cccgctcgtg ctgatcccgg tgccgctggc ggcggcgcgg
    15601 cgggcgcggt cgtggcggct ccgcgccatc gccgaccagc tcggctggcg ccgcccctgg
    15661 ggccggcgcg tcgggcgcgc gctggccgcg gcgggcttca tcccggtggc tgtacttttc
    15721 atcctgcggg cgagtctggg ttttgactgg atcgacacgt ttcttacggc gctgcgccat
    15781 cagcacggga cgctcctgcg cggcatcgag cggagctacg ccacctggct cggctggaac
    15841 ctctatgact ttctgctctt cgccggagga acggtcgcta cctttggcct ggtgtttctg
    15901 ggcggcgccg tgcggcgagg ctggcgccgg ccgcgccggc tcctcccaat ctggttcgcc
    15961 tttccattcg tcctgctgct gttggacctg tcggggctga cgccgggcga gaccgggcgg
    16021 atctggctct tcctggcgcc cggtctcgtc tgggccgccg cgatcgaact ggcccgccgc
    16081 gcgcgcgccg gctggccgac cgcgctcttc ctcgtcctgc tcgcccagtg gatcttcctg
    16141 ctcgtcctcc gcacgcggat gcactacatc ggctggtgag acgccactcc gcctgaccag
    16201 aacgcatcaa tccaggcata ccgcggatgc cgcaccgtga acgcaaacga acagcgattc
    16261 cgttgaatcg tccgtttacg aacggcaatc ggcttgaatc cagtttcgtt tccgtccctt
    16321 cagccttgcc gccgcgccgt cttgcaagcg cctcgcgccg ctgccaaacc actctctcta
    16381 ttgactttcg gcgcgttgtc gcgacctgtc gccgcttgcg gaaatctcaa gcccggcggc
    16441 gcaagcgtgc gtgcggcacg agcgttgcac ctcttcctcg atgacggatg cgtgaatcgc
    16501 aacgatctca aacggggagg aacgcaaaat gaagagtttg gttaaatcca tcggcgcggc
    16561 cagtcggttg gcccgcctgc tggcattggg cgcggtggct gcctgggtcg cctcgcccgc
    16621 cggcgcgcag ccccccgctc cgccggacga cctggcggcg cccgcctttc ccgaggatgc
    16681 cggcgacgcg ttgcccggcg gcggcttcga gcccggcttc ggcccggcga tgggacccgg
    16741 catgggaccc ggaccgggga tgggcccggg gttcggcata ggccgcggat tcggcgcccc
    16801 cggcatgggc aggggcatgg gacccggcct cggacggggt ccgggggggg gccgcgccag
    16861 ttgctgggcc gcccagacgc tgctccaacc cgacgtccag gccacgctcg gactgaccca
    16921 gcagcaggcc gaccggatcg agcagacgga ctacgacacc cagtcgcggc tgatcgccaa
    16981 acgcgccgag ctggaacagg cacagcttga actgcgccgg ctgtgcgacg accccgacat
    17041 tgaccggacg gccctgatga cacagatcga ccggctgggc gcgctgcgga cggagctgca
    17101 caaactcaat gccggacgcc tgctggatgt aagggaaacc ctgacgcccg agcagcgcca
    17161 gcaggcccgc gaggtgatcg gccagcgcgc gctcgtgcag cggtcccagc aacggccgtt
    17221 catcggcgga cgtcgcggcg cgatgcgcgg cgggccgggc cccggcggac ccggcatggg
    17281 ctggaaccgc ccgggcatgc ccggcgggca agggcaacag ccgccgctcg ctcccgcgcc
    17341 tccggctcag taatccgcac cgcaaggcat ggctctgcgt ccccgcggca ccccgccgcg
    17401 gggacgcccg cattgccggc atctgtctca gtatcggtat cggtatcgca atcgggatcg
    17461 ggatcgggac cgggaccgaa caggagctca accgtctgtt ggccttcgtc gctcgtccct
    17521 cggctctcga aaacctgaaa aaaatcagct cccctcagtt caaactcgcc aattcctctt
    17581 cgatcgcctg catcacgggc gcgagcgtcg cggcgctgaa atcgaaccgg atccccgccg
    17641 ccgcgaacaa ctccggcaac ggacgcgatc cccccagcgc caggccgttc agataatcct
    17701 ccagcgcctt gcgcggttcc cggcggaact tcagccagat ctgcagcgcg cccaactgcg
    17761 cgatgccata ctccacgtag tagaacgggt gcaggaagat gtgcaactgg cgctgccact
    17821 ggttgcgcag ttcggcgtcc agcccgctcc agtcgatctc cggatcgaag cgctcctcca
    17881 gccgcagcca ctccgcctcg cgctccgcca ccgtgtgctc cgggttcaga tagatccagt
    17941 gttggaacgc gtcgaccgtc gcgatccacg ggaagatcga gaggatctgc tccaggtggc
    18001 gccggcgcgc gcgccgcgcg tcgccctcgt ccggatagaa tcgctcgatg tgcttcatcc
    18061 cgaacagttc catcgccatc gacgcgacct cgcagaattc aatcggcgcg ctgcggtagg
    18121 tcagaatctc ctggtcgcgc gcggcgaacg catggaacgc atgccccccc tcatgcagca
    18181 gcgtttccac gtcgcgatgc gtgccggccg cattcatgaa gatgaacggc aggcgcactt
    18241 cctccagcgt atactggtaa ccccccggcg ccttgccctt gcggctctcc aggtcgagca
    18301 gccccaggtc gcgcattttc tgaaactgcg cggcgagcgt cggcccgagg tcggcgaaaa
    18361 tcgcgccgca gcgctgcgcc agatcctccg ccgccgaaaa cggcttgagc ggcgctttgc
    18421 catcgacgtc gaccgacagg tcccacgggc gcaaacgctc cagcttcagt ttctcccgcc
    18481 gcacccgccg ctgggccgcc agtcgcggca ccacctcctg ctcgatcgcc cggtgaaacg
    18541 tcaggcagtc ctccggcgtg tagtcgaagc ggttgtaggc ccgatgctga taatcgcgga
    18601 aattcgcgaa gcccgcgttg cgcgccatcc gcgtccgcag atcgatcagt ttggcgaaca
    18661 ccgcgttgat cgcgtcgcgg tccgccagcc gccgttcggc cacaagccgc caggcgcgct
    18721 cgcgcacgcc gcggtcggcg tcctcggtgt agaccgccat ctgttgcagc gtcttctcct
    18781 cgccttccca catgacggtc atcgcgccgc tgatcttctg atactcgttg ccgagcttct
    18841 gatcctcggt ctgaagcggg atgttctcct cgcgatagag ctcgacctgg ttctgcagcg
    18901 cgcggtcgtg cacgcgcagc cagtcgcggt cgagcgcctc gcgcgccggg cattccagat
    18961 acagccgggc gatcttgtcg tgccacggct tgatcttcgg cagaatgttc tcgacgtaat
    19021 gcaggtaggc cgcctcgcgc tccttgtcgt cggtctggca ggtcatcgcg atgtaacggc
    19081 gcgcgtattc ctcatcgatc accgcatcca gctcgctctg gcggcgcagc cactggcgca
    19141 ggtgctccgg cagctcgatc atgcggatct ggctggccag atcgtcgaac agcggttcaa
    19201 gctggctcca atcgcccaga tcggtcacgt gccgggtgaa atcgcgtggg aattcctcgc
    19261 cgcgctttgc ctggatcgca ctggtttcgg ccatggtcct gttgcaactc ctcacaaact
    19321 cggatgcaag ccagccagtc tttcatgccg gcgcgagcga aggcaagcgc ttgctccgcg
    19381 catgcctcgc cggcggcgcg gcgcgacggg tttcagaatc aagccgcgga ggagtttcag
    19441 atcggcacgg gcgtttcttc caggatcgcc cgcaccgccg aggagccttc ccccagactc
    19501 tcggccgtct cctcgaacgt ccggatgatg cccagccgat gcgccagcac cgagaccgcg
    19561 accgccttga cgtcgtcggg gatggcgtaa tcccgcccgt ccatgtaggc gttggccttg
    19621 gcgcagttgc gcagcgccag cgccgcccgc gtcgacgcgc ccagctcgat cagcggcgag
    19681 tggcgcgtcg cgttgatcag gttcacgatg taatcgacca gcgaatcatc gacgcgcacg
    19741 cgccgcaccc cttcctgcat cgcccggatg cgctcgctgg agagcaccgg cttgaccatc
    19801 gtcagatcgc ccgtcgaccg ggtgttgcgc agaatctccc gctcatactc cgcgtcgggg
    19861 tagcccagat gcagccgcat catgaaccgg tcgagctggc ttttcggcag cggaaacgtg
    19921 ccgtggctgt cgaacgggtt ctgcgtcgcg atcaccatga acggctgcgc caggtcatag
    19981 gtgttgcgat cgatgctgac ctgcgccgtg ttcatcgcct ccagcagggc cgactgcgtc
    20041 ttcggcgtcg tccggttgat ctcgtcgacc agcacgatgt tggcgaagat cggcccggcg
    20101 cgaaatacga acgccttctt gtcgttgtcc cagaccgaca cgcccagaat gtccgacggc
    20161 agcagatccg acgtcccctg aatccgctga aacgtgacgt tgatcgacat cgccaggcac
    20221 tgcgccagcg tcgttttccc cacgccggga acatcctcga acaggacatg cccgccggcc
    20281 agcagccccg taatcgccat ccggaccgat tgatgcttgc cgcggatcac cagctcgata
    20341 ttctgctcga gccggcgcag atcgtcgtgg gccgccttgg cgctgagcgc ttcctgcatc
    20401 gatacagctc ctctcgaacc cgataatccg ccccacatga tagtgtcatc ccgcttcgcg
    20461 gacaagat
//
LOCUS       DAOPCO010000039        20200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2788_length_20200_cov_4.880913, whole genome shotgun sequence.
ACCESSION   DAOPCO010000039 DAOPCO010000000
VERSION     DAOPCO010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 20200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20200
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_20200_cov_4.880913"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..962)
                     /locus_tag="PLS90_04885"
     CDS             complement(99..962)
                     /locus_tag="PLS90_04885"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK01772.1"
                     /translation="MATEQSTDPRLTLEISERDLPVMSVVAGQALELLADDRTTNRQI
                     EQLIGRDVALAQRILAVANSPFYCGLRASQTISGAVMRLGIRQLRRVILTAATGELFD
                     TGDPVMQRIWSHSLATAMAAARLAEAIAPDLAETAYVAGLLHDVGKIVIYQQHPAVYG
                     DLLAEADAAQRPLHDLEQERLAYFTHASVGGLAIRRWQLDPALTEAARAHHLLETEIP
                     SWLQHHALVSLVGLANRLVAAAGLGGPGPSEAQLIDSPIAQTLHLTPERLSGLARRVD
                     QVLEENRAHLR"
     gene            1595..2122
                     /locus_tag="PLS90_04890"
     CDS             1595..2122
                     /locus_tag="PLS90_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01773.1"
                     /translation="MKPRALSTVGCGLLTLVLAGAAWADITSKPRMISAQRGAPLLIS
                     LDVDATEAEAVAGINGRLTYDPAIFHNPAIGLQVGAAGFTALGNEVAPGDFRFVIYAN
                     PTATMNLNLPVAEFNIDVDPLAPFNTTQTLSFDTAAAGSPEGQSFAAQGKSVSFADFV
                     VNINANAAEAWAVYE"
     gene            2276..>2504
                     /locus_tag="PLS90_04895"
     CDS             2276..>2504
                     /locus_tag="PLS90_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01774.1"
                     /translation="MDPLLESVRDQVQFTWPAWLIIAAYMIFTTWLGHRLSGRQATIR
                     DFFLGGRRLPWYAVCGSIIATELSAMTLVVAP"
     assembly_gap    2505..2514
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2515..4073
                     /locus_tag="PLS90_04900"
     CDS             <2515..4073
                     /locus_tag="PLS90_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01775.1"
                     /translation="FLWSATGDMSYAVLGVGTILARIVVGYWFVPHYYSEEIYSPYEY
                     IGNRLGERAGRTTSLLFMLGGVLGQGTRVLLTAVVLQVVTGISIFWSIWIVGLVAVLW
                     TWMGGITTVIWTDVIQFVVFLFSSLLMMLIVLWEFPPTGVVSPGLVFNLAWEEGKFHW
                     LNFEFDLTQSYTVWAGLIASTIGGLAAYGTDQMLVQRLFCCRDPREARKAIIWSSAGQ
                     IMMVICLLTGVGLWAYYQRSGLPDTPNAWEATQIAENTNRLVPVFIKFRMPWYLGGIM
                     VAGIFAAAISSLDSILAALAQQTLAWTRGRRGEQQGDVREIALSRLYVILWAIVLCGM
                     ATVFQMLIVRELSSAEASGTFGSGLLIELALAVVGYTSGGILGFFLLALVPSWRVEGR
                     GLEWAAALSVMTIFAITQHAGWAFTALLAAGAALIAAALGLLPERRAAFTLKLLPFIA
                     GILLLNRVQTDGLRFAWPYGADGAGEAAAFIRLGWPWFVPLGCLVMVTAAWTLCRRQG
                     GRGAEEPVRG"
     gene            complement(4092..5129)
                     /locus_tag="PLS90_04905"
     CDS             complement(4092..5129)
                     /locus_tag="PLS90_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPK01776.1"
                     /translation="MHLVYHPRYLDHLQGGAGHPERPERLAAIEARLRAAGFWRDLLE
                     PGPVDDADVLRVHRPAYLRRLAAFGEGDWDADTYVRPETPAIARLAAGGALLAVDTAW
                     RDAVPALALLRPPGHHATPDTAMGFCYLNNAAIAAACLFARERRPLAIVDLDVHHGNG
                     TQDIFITSRDILFISTHQYPHYPGTGPATETGAGDGAGHTVNLALPAGSGDGTFHLAF
                     DRVIEPILRQFRPAMLLVSLGTDAHARDPLGDLTLSSAGYLHLGRRLNALAAELCGGR
                     IAYLLEGGYDLEALADVITGLVALARGEPFQPDFSIPTDPHCVGRRAIEATVQYQAPF
                     WQLDPAPQPPE"
     gene            complement(5158..5637)
                     /locus_tag="PLS90_04910"
     CDS             complement(5158..5637)
                     /locus_tag="PLS90_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK01777.1"
                     /translation="MPEKKATQQQIVAELKKSYFMEIETVINYLANSIHLDGIRAEEI
                     RKALAADVQEELTHAQTLARRIKVLGSGIPGSLDLTMEQKSLQPPRDLTDVVSVIKGV
                     MEAEQGAIEQYTKLIEMCDGIDWVTQDICIQLMGDEQEHLRLFKGYLTEYSKKEVKA"
     gene            complement(5822..6496)
                     /locus_tag="PLS90_04915"
     CDS             complement(5822..6496)
                     /locus_tag="PLS90_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPK01778.1"
                     /translation="MKKVRACWLMAGFLFLIPVFAGAQQGALVKADTLTLRGSASTSA
                     RSIAKLNKYQPVKVLERKNEWARVRADGNEGWVLAEYLTRTGFVWIDHDKLNVRRGPG
                     TEYAVIMNYGRHFPVWVVDLARNGWILVQDYEGDKGWVHPNLVELEPPYVITKLGKCN
                     VRTGVGTEAAIAFTAERGVIFQVLEEKDGWLHVRHADGDEGWMSAKIVFGWLDADAQS
                     TQSGSG"
     gene            complement(7090..10584)
                     /gene="dnaE"
                     /locus_tag="PLS90_04920"
     CDS             complement(7090..10584)
                     /gene="dnaE"
                     /locus_tag="PLS90_04920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPK01779.1"
                     /translation="MADLGPFVHLHVHSHYSTLDGANSLKGLIEQTKQLGMGAVALTD
                     HGNLFGAYAFQKAAQEGGVKPIIGCEAYFSPTSHTDRTSNKARTRYHFLLLCESVEGY
                     RNLSRLSSLGFLKGYYYKPRIDLELLERYHDGLIGTTTCIQGPVCQAILNGDLREAQR
                     QIDVFAQIFGRGHFFVELMDHGMPDQKTVNNGLLELAKKNDLPLIATNDCHYLTAAAH
                     DSQDVLMCISMGKTLNDPQRMRFEQNEFYLKSPDQMRSLFGHLNGAIENTVAIAERCN
                     CVIPTNQKLLPRYRTPDGMDGPTYLRLLVQRGLDKRYGGRPEERHRRQADFELGVIEK
                     MGFVDYFLVVWDFIRWAKDQGIPVGPGRGSGAGSIVAYALEITDLDPLEHGLLFERFL
                     NPERVSMPDFDIDFCFERRGEVIDYVRRTYGQDCVAQIITFGTIKARNAVRDVGRVMG
                     VALPKVDQVAKLIPGGPKVTIDAALGKDSRHPELASPELQTLYNDDPEVRELLDRARG
                     IEGAIRQPGTHAAGVVICDQPLIDLIPLYRPSDNDPDAMAVTQFTMTEVEEIGLLKMD
                     FLGLKNLTIIDRCLKGISTRLGRTITAADIPLDDEATYRLLQQGKGLGVFQLESSGMR
                     DLLLNFKPTRFSDLVALISLYRPGPMDNIPEFIARKEGRREITYDHPDMEPILKETYG
                     LFVYQEQVMQVAQVLAGYSLGGADLLRRAMGKKKKEEMAKQRRTFIDGARSRGIDEPT
                     AERIFSTMEKFAEYGFNKSHAAAYAVITVQTAWLKAHYPVDFYAALMTNEVGGDDAKL
                     GMYFEEARDEGIRILPPDINSSGVHFTPDGGNIRFGLAAIKGVGESAVRAMIEERSAN
                     GPFTSLQDFITRVDKRLINSRVVEALISCGAFDAFGHNRPSLLAVLPRLMEVAGTARR
                     DADDPQTSIFDMMTEEHTAGLRAELPIPRLPDWGDKERLETEKELVGFYLTGHPLERF
                     APDFAAFSTASAREILAMKPGDDVEWVGLIKRIVTRMDKNGRQMAFVECEDMTGPMEC
                     TFFADAFDACREAIREGEVIWVRGKVDSWRDNIKIRVHAGKPIDQLRAERIRTLEVQL
                     TWQQVTEANLTRLSEIAARHRGRRKLWFVIGDNGDEVRVEAGPGFGIAPCTALIHDLQ
                     DTHLLQGLRYIANGNGSS"
     gene            complement(10969..11664)
                     /locus_tag="PLS90_04925"
     CDS             complement(10969..11664)
                     /locus_tag="PLS90_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK01780.1"
                     /translation="MSASQLTKPGQAPARLPAVEPWHPGLRGFYRFTHFVMSTVARLY
                     CRREVIGLEHFPPSGPALILPTHSSFIDPPMVGAALNREIYALSRAEILEVPLLGWLC
                     RRFYHGVPIRRGGGDREALRLCRRILRAGWPLLYFPEGQRTPTGTMQPLQRGWILILD
                     DLPGVPWIPVQMQSTYHIMRRGQLFPRPRKLRIVFGKPRPLPERRPGENSRQWHARCN
                     AELEQALRDLGAV"
     gene            complement(11661..13127)
                     /locus_tag="PLS90_04930"
     CDS             complement(11661..13127)
                     /locus_tag="PLS90_04930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01781.1"
                     /translation="MTHPPRRLLLLGAGPVPDGGSLHAPGVANRLLHFLLPALKAGHE
                     ALVLTIEPDATALDRDEPRPEERSRGALRWRHLALTVEDLKSHPAIPSLVAAWAPERV
                     VGAGTLQAAARACALAADAPVWADFFGDPLAEIHARNEFLPPGPERLDSTLLVWELML
                     RTLLRADAFSVVSRRQRDALLGQLALAGRLAAAPEARHPAGPPIEPMPCCLESLDDRP
                     PPSADERARAFASAGLPPAARVALWSGGFNAWADPATLVAGIERAMARRPDLHLVATG
                     GALPGYLARLFELFRERVDASPHRDRFHALGWLPLAEANRWLALADVALLTDRPCAET
                     HLGARNRLLYHAAVGCPVVASTGSEVVAEMAAAGALAPFPSANPDALAGEVLRLLEND
                     AERRRLGEAGRQFCRRQYLFEHTCAGFLDWVARASPAAPPAPDPSDPRQWIARYLTAW
                     AQPGEADGGARRPRLLGRLLARLRAPNPRSPAEPPSHP"
     gene            13387..14670
                     /locus_tag="PLS90_04935"
     CDS             13387..14670
                     /locus_tag="PLS90_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK01782.1"
                     /translation="MIRQHHTTHRHTLGCGMPLLLLENHEAAVATADIWVRTGAADEP
                     EDLSGISHFLEHMLFKGTEKWGLGEIERAIESVGGVSNAGTSYDFTHYYVTLPAARIR
                     TGIEMLAEMVRHSTLDAAELEKERLVILEEYRRKQDNPIGVLYELLYGELFEAGPYHL
                     PVIGSEETIRAIDRPAMLDYYHRRYGPEGAVLVVAGDIEPERAIEWAEAAFAGFDRRR
                     EELTRRAPVRYGRGKRVHERKPTGGEVYVAYAFPAPGMQATEEVLPLDMAQFILGSGR
                     AARLHQELKEKRGLCSSISSYYPTHAHDSLFVFVATCQPGQREGLRAALLEELRRFAA
                     EPPTPPAMKRARRLLASSHLFSMETSSGAASNIGYYYTLSGSTEFFDSYLEKLEALTA
                     EQVSAATARVIHPERLEESLVEISVGPNGELKGEG"
     gene            14670..15938
                     /locus_tag="PLS90_04940"
     CDS             14670..15938
                     /locus_tag="PLS90_04940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK01783.1"
                     /translation="MEAAHGGSSGPVRFERMKIVGADALLAPRPGTGLLALAAIIRRG
                     SADEREGEHGLASFTASMLLRGTQRRSSEQIAFDLESLGAIAGEGDGMDTCSLNVRCS
                     TEAAPRALEILFESLREPAFDAREHEVHRQELLAHLRMIEDEKFSYTYREYVKAMFTG
                     HGYGHASEGELADAERITPADCRRWHAEMIRPETLLIAAVGDFEVGALRDQLAGLLAD
                     WPVNDGPVRPRIATQPPADHPRELELRKPELNQGFIVGGFRTPPITDPDYAALRLGSA
                     ALGEGFAGRIFTELREKRSLAYALGATLRSYRLTGHQVLYIGTKPESIEEARAGLLRE
                     ADYLKHNLLSDEELNRAREYVLGKFVMGQQSHAQRVSHLAWWEDAAGDAAEGWRYPER
                     LRGVTAAQVREAARKWWVEPTMAVLRPEAG"
     gene            16207..16974
                     /locus_tag="PLS90_04945"
     CDS             16207..16974
                     /locus_tag="PLS90_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01784.1"
                     /translation="MGNHAAIEVNQLHIRYGRGARRHLAVAGLTFQVEPGETVGFIGP
                     NGAGKSSTIKTLMGFQYPTEGTVRLFGEQAGSIAARRRIGYLPEVSLYYPFMKAREVL
                     ELYGGLQGLDRATLKRRIPELLERVGLAGKGETLLKHFSKGMQQRVGIAQAIIADPDL
                     LIFDELSSGLDPVGRYDLREVLLELKRRNRTIFFSSHELYEVESLCDRVIMIHGGRKV
                     ADAAVPELMEQLKAGNGGRGQSLEEFFMGLIRPAEAA"
     gene            16974..17747
                     /locus_tag="PLS90_04950"
     CDS             16974..17747
                     /locus_tag="PLS90_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK01785.1"
                     /translation="MLRRQWLIARTIFVEALRRREIYAIVLITAGMLLLASLLRFFDL
                     QGLHKFYHEVSLKVMTTATVLTVIVLAARQLPREFERRTIYTLLAKPIARWEFLLGKY
                     VGVVLAGLFCLGLFMGVFCLGRLFTQAPLDWRLFGQFLFMQGLAIGCLAALSFLLSML
                     MGLDAAIVTAMLIYLLGQVMTNAMTVVYEFTGAAGRAVLITLNYLVPQPALFDLSAKV
                     VHEWPPVPAWVLGLATLYCLMFVIPYLGLSYALFRRRAL"
     gene            17770..18573
                     /locus_tag="PLS90_04955"
     CDS             17770..18573
                     /locus_tag="PLS90_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK01786.1"
                     /translation="MSLAAQETLLSMETPRGPRPWGPLARAAWIQAVRRSEIHVLLIL
                     LGLYGLAALILRLIGIESAQTARFIAGLGLQLGSCLAMLLVIILIGRQVPTEIDQRTI
                     YPMLAKPVSRMQLLLGKALPIWLAGVAALGLFVGVTLLITPRLPIQHPVVLAEALVLR
                     AGGLAMAGALAMWLSLRLPMAVAMLATAAAAFFGGMAGNFAAQAGGRLGALAARFIPD
                     FSLLDQFQRYVDGGRPLSLAEIAGLLLYAALWTFLFSVLAGTTFRRMAL"
     gene            18585..19607
                     /locus_tag="PLS90_04960"
     CDS             18585..19607
                     /locus_tag="PLS90_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01787.1"
                     /translation="MERLNQAPRPGGGYSMLGTLTLLAGLGAIGLLRPALEPAKAWQP
                     AFVMTEAVAEQVNTSPFALILGEIRASAADLMWVKTERYLHRGVAYAPHLNADNLART
                     GETVNRAEESRSAGAEHVHDEHCDHEADADGAGADAGHGAEVALIPDRESDYRGFIGV
                     LHREVQPWEGGDQPHEHSGGEQLLPWYRLLTLSDPHHWRGFMIGAWWLMQQREEGAVS
                     LAEARSFIEEGIRHNPDVFQLHLMHGRVLTRLEEWDGAIGAFRRAVEVGLKLRPEGGV
                     EEPPVWTLSEEEDFEMAVRYIAMIQARKLNDIPAALATIEWGLRLVPGDGPLTHLRED
                     LTTAGE"
BASE COUNT         3130 a         5609 c         8049 g         3402 t
ORIGIN      
        1 tgaggggtga gcggtggagg ggggcggctc cgcaccgggg gggtggtgcg atcgagcatc
       61 ggccaaaccg ggtgcggggg aaggggggga ccctcgggtc agcggaggtg agcgcggttt
      121 tcttcgagga cctgatcgac gcggcgggcg aggccgctga ggcgttcggg cgtcaggtgg
      181 agggtctggg cgatgggcga gtcgatcagt tgcgcttcgg agggcccggg gccgcccagg
      241 ccggcggcgg caacgaggcg gttggcgagg ccgaccagac tgaccagcgc gtgatgttgc
      301 agccaggaag ggatttcggt ctcgagcagg tggtgggcgc gggcggcttc ggtcagcgcc
      361 ggatcgagct gccagcggcg gatggcgagg ccgccgaccg aggcatgggt gaaataggcc
      421 aggcgctcct gttcaaggtc gtgcaggggg cgctgagcgg cgtcggcttc ggccagcagg
      481 tcgccgtaga cagccgggtg ctgctggtag atgacgatct tgccaacgtc gtggagcagg
      541 ccggcgacgt aggcggtttc ggccagatcg ggggcgatgg cctcggcgag gcgggcggcg
      601 gccatcgcgg tggccagcga gtggctccag atgcgctgca tgacgggatc gccggtgtcg
      661 aacagttcgc cggtggcggc ggtcaggatg acgcggcgaa gctggcggat gcccaggcgc
      721 atgacggcgc cggagatcgt ctgcgaggcg cgcaggccgc agtaaaaggg ggagttggcg
      781 acggcgagga tgcgctgggc gagggcgaca tcgcggccga tcagttgctc gatctggcgg
      841 ttggtcgtgc ggtcgtcggc gaggagctcg agggcctggc cggcgacgac ggacatgacc
      901 ggcaagtcac gctcggagat ctcgagcgtc aggcggggat ccgtggattg ctcggtagcc
      961 ataagcgcaa cgacttccga cccagggaag ggggcaaaat cggccatcgg cctccggaaa
     1021 ggaggttctg gagaatctat cggcgggcgg gggggataga atgagcggga tccggagaat
     1081 gaggacaggg ggaggggggg tggaaggggg cgaggacgag gggcgagagg tgaaaaacct
     1141 gattggggtg tgttttttgt tgcgcggcgg cggggtccgt cccatgatgg ccgaagctga
     1201 gaaaggaggt ggtccagtca tgatagttcc atgtaccagc gaggtggctg aaacgtaact
     1261 ggcttgcctg cttgagaaag cggcttggga tcaacgggca aggcagccgt tagacccctc
     1321 agtccaccga agattgcgcc cgcgggttgg ccgccattgg ctcccgcggg cttttctttt
     1381 ggggggggcg gtgggagggg aagggacgga agggacatga gggacgtaag gatcgatggt
     1441 tgatcctcaa gagtccgcat ttccgttgac gcaatctgac acatcgggtt accttgctga
     1501 aggagcggcg ggggctgttt cacacggatt tcattcgccg cgggacgatg cgaatgcatc
     1561 gtccagagcc ggagcagggg gaaggcagga gcatatgaaa ccgcgcgcat tatcaacggt
     1621 cggctgtggt ctgttgacgc tggtgctggc cggcgcggcg tgggccgata tcacgagcaa
     1681 gccgcggatg atcagcgcgc agcgcggggc gccgctgctg atcagcctgg acgtggatgc
     1741 gaccgaggcg gaagccgtgg cggggatcaa cgggcggctg acgtatgatc cggcgatctt
     1801 ccacaacccg gcgattgggt tgcaggtggg cgcggcgggg ttcacggcgc tgggcaacga
     1861 ggtagcgccg ggcgatttcc ggtttgtgat ttatgccaac ccgacggcga cgatgaatct
     1921 caatctgccg gtggcggagt tcaacatcga cgtggatccg ctggcgccgt tcaacacgac
     1981 gcagacgctg agcttcgaca cggcggcggc ggggagcccg gagggtcaat cgtttgcggc
     2041 gcagggcaag agcgtgtcgt tcgccgattt cgtggtcaat atcaacgcga atgcggcgga
     2101 ggcgtgggcg gtctacgaat aacgcccgcc gggcgcgacg gcgtcacgca aaccgatccc
     2161 ccgtctcagc gcgaggcggg ggttttcatt tgcgccggtt tggatgaggg gacgaaggtt
     2221 ttctttcgcg gccgaacgtg ctattgcgtg gtgaaccccc aaccccggag cacgcatgga
     2281 tccccttctt gaatccgtcc gcgaccaggt gcagttcacc tggcccgcct ggctgatcat
     2341 cgcggcctac atgattttca cgacctggct gggtcaccgg ctgagcgggc ggcaggcgac
     2401 gatccgcgac ttttttctgg ggggtcggcg gctgccgtgg tatgcggtct gcgggtcgat
     2461 catcgcgacg gagctgagcg cgatgacgct ggtcgtggcg ccggnnnnnn nnnncgttcc
     2521 tgtggtcggc gaccggcgac atgtcgtatg cggtgctcgg ggtggggaca atccttgccc
     2581 ggatcgtcgt cggctactgg ttcgtgccgc attactattc cgaggagatc tactcgccct
     2641 acgaatacat cggcaaccgg ctgggggagc gcgccggccg cacgacgagc ctgctgttca
     2701 tgctcggggg ggtgctgggc caggggacgc gcgtgctgct gacggcggtg gtgttgcagg
     2761 tcgtgacggg gatctcgatc ttctggtcga tctggatcgt cgggctggtg gccgtgctgt
     2821 ggacgtggat gggagggatt acgacggtga tctggaccga cgtgatccag ttcgtggtgt
     2881 ttctgttttc gtcgctgttg atgatgctga tcgtgctgtg ggagtttccg ccgaccggcg
     2941 tggtcagccc gggcctcgtg ttcaacctgg cctgggagga aggcaaattc cactggctga
     3001 atttcgaatt tgacctgacg cagagctata cggtgtgggc ggggctgatc gcctcgacga
     3061 tcggcgggct ggcggcctac gggaccgatc agatgctggt gcagcggttg ttctgctgcc
     3121 gcgacccgcg cgaggcgcgc aaggcgatca tctggtcgag cgcgggccag atcatgatgg
     3181 tgatctgcct gctgacgggg gtggggctgt gggcgtatta ccagcgcagc gggctgcccg
     3241 acacgcccaa cgcgtgggag gcgacgcaga tcgcggagaa cacgaaccgg ctggtgccgg
     3301 tgttcatcaa gtttcggatg ccgtggtatc tggggggcat catggtggcg gggatcttcg
     3361 cggcggcgat cagttcgctc gactcgattc tggcggcgct ggcgcagcag acgctggcgt
     3421 ggacgcgggg ccggcgaggc gagcagcagg gcgacgtacg ggagatcgcc ctgagccggc
     3481 tctatgtcat cctgtgggcg atcgtgctgt gcgggatggc gacggtgttc cagatgctga
     3541 tcgtgcgcga gctgagcagc gcggaagcga gcgggacgtt cggctcgggg ctgctgatcg
     3601 aactggcgct ggcggtggtg ggttacacca gcggcgggat tctggggttc ttcctgctgg
     3661 cgctggtgcc gtcgtggcgg gtcgaggggc gcggactgga gtgggccgcg gcactgagcg
     3721 tgatgacgat cttcgcgatc acgcagcatg ccggttgggc gttcacggcg ctgctggcgg
     3781 ccggcgcggc gctgatcgcg gcggcgttgg ggttgctgcc cgagcggcgc gccgcgttca
     3841 cgctgaagct gctgccgttc atcgcgggga ttctgctgct gaaccgcgtg cagacggacg
     3901 gcctgcggtt cgcctggccg tatggcgcgg acggggcggg cgaggcggcg gcgttcatcc
     3961 gactggggtg gccgtggttc gtgccgctgg gctgcctggt gatggtgacg gcggcctgga
     4021 cgctgtgccg gcggcagggg gggcgcggcg cggaggagcc ggtgcgcggc tgagcggggc
     4081 gcgacgaaga attattccgg cggctggggc gccgggtcga gttgccagaa gggggcctga
     4141 tattgcacgg tggcctcgat cgcgcgccgg ccgacgcagt gcggatccgt ggggattgag
     4201 aaatccggct gaaagggttc gccgcgggcg agggcgacca ggcccgtgat tacgtcggcc
     4261 agcgcctcca ggtcgtaacc cccctcaagc aggtaggcga tgcggccgcc gcagagctcc
     4321 gcggccaggg cattgagccg gcggccgagg tggaggtagc ccgcgctgga gagggtcagg
     4381 tcgcccaggg ggtcgcgcgc gtgagcgtcg gtcccgagcg agacgaggag catcgcgggg
     4441 cggaactggc gcaggatggg ttcgatgacg cgatcgaacg ccaggtggaa ggttccgtcg
     4501 ccgctgccgg cgggcaaggc cagattgacg gtatggccgg cgccgtcgcc cgcgccggtt
     4561 tcggtggccg ggccggtgcc ggggtaatgc gggtattgat gggttgagat gaacaggatg
     4621 tcgcggctgg taatgaagat gtcctgggtg ccgttgccgt gatggacatc gagatcgacg
     4681 atcgcgaggg gccggcgctc gcgggcgaac aggcaggccg cggcgatggc cgcattgttc
     4741 aggtagcaga agcccatcgc ggtatcgggc gtggcatggt ggccgggggg gcgcagcagg
     4801 gcgagggcgg ggacggcgtc gcgccaggcg gtgtcgacgg cgaggagggc gccgccggcg
     4861 gcgagccggg cgatcgcggg ggtctccggg cggacgtagg tatcggcatc ccagtcgccc
     4921 tcgccgaagg cggccaggcg gcgcagatag gcgggccggt gaacgcgcag cacgtcggcg
     4981 tcgtccaccg gccccggttc caacaggtcg cgccagaagc cggccgcgcg caggcgagcc
     5041 tcgatggccg ccagccgctc gggccgttcg ggatggccgg cgcctccctg cagatggtcc
     5101 aggtaacgcg ggtgatagac aaggtgcatg ccgcggaccc gggagacgct tcggcgatca
     5161 ggctttgact tccttcttag agtactcggt cagatagccc ttgaacaggc ggaggtgttc
     5221 ctgctcgtcg cccatcaact ggatgcagat gtcctgggtg acccagtcga tgccgtcgca
     5281 catttcgatg agcttcgtgt actgctcgat ggcgccctgt tcggcctcca tgacgccctt
     5341 gatgaccgag acgacgtcgg tcagatccct ggggggctgg agggatttct gctccatcgt
     5401 caggtcgagg gagccgggga tgcccgagcc gaggaccttg atgcgcctgg cgagggtctg
     5461 ggcgtgggtc agttcctcct ggacgtcggc ggcgagggcc ttgcggatct cctcggcgcg
     5521 gatgccgtcg aggtggattg aattggccag gtaattaatg accgtttcaa tctccatgaa
     5581 ataggacttc ttgagctcgg caacgatctg ctgctgagtg gccttcttct caggcatggt
     5641 cggtaaccct ttccagccgc ctgcgggcgg cgcggtgttt acaacgcgcg ggcgagcccg
     5701 tggagagaca agtcaagttg cgccaagttg atgcgcggcc accggggcgc gcgccgtgcc
     5761 agcggctcgc gagacacgct gtgtgtgaac aaactttgtg ccgattggac ggcgaacggg
     5821 ctcaaccgga cccggactgg gtggattggg cgtcggcgtc cagccagccg aagacgatct
     5881 tggcggacat ccagccctcg tcgccatcgg cgtggcgcac gtggagccag ccatcctttt
     5941 cctcgaggac ctggaaaatc acgccgcgtt cggcggtgaa ggcgatggcg gcctccgtgc
     6001 cgacgccggt gcggacgttg cacttgccca gcttggtgat gacgtagggc ggctccagct
     6061 cgaccagatt ggggtggacc cagcccttgt cgccttcgta gtcctggacg aggatccagc
     6121 cgttgcgggc caggtcgacg acccagaccg ggaaatggcg gccgtagttc atgatcacgg
     6181 cgtattcggt gcccggcccg cggcggacgt tgagcttgtc gtgatcgatc cagacgaagc
     6241 cggtccgggt cagatattcg gcgaggaccc atccctcgtt gccgtcggcg cggacgcggg
     6301 cccattcgtt cttgcgctcg aggaccttga ccggctggta tttgttgagc ttggcgatgc
     6361 tgcgcgccga cgtggaggcg gaaccgcgca gggtcagggt gtcggccttc accagggccc
     6421 cctgctgggc gccggcgaag acggggatca ggaacagaaa gccggccatc agccagcacg
     6481 cacggacttt cttcatcgta aggcgcaatt ccctcggaaa tcttgtggga ggccggcgcc
     6541 gcggggaccg cgcgcgtcgc gccgggcgtt tcccttccac aagactaata caggggggtt
     6601 ttgtttcaca acgcctgaat ggccggtggg ccccttgcgc ggggaggacg atacggttgt
     6661 gtccgattgt gtccgatggc gcaagcgatg gcgatagcga tagcgatacc gtatcaggac
     6721 gaaagggagg ggtgagccgc gcgatggtca gaagcggggg cgcctcgccg gccatccctt
     6781 gcaggggttt ggttttgcgg gggtggcaac gcagggttcc ggcctgcggc cgtcaccctg
     6841 cgcttgactc cggtcgtccg cagcgcggac ttagccggca accgagtcga agccgcctgc
     6901 attggatttg gaatccttga gtggaacgag ttggggaatg aaaggattgg aggtggagaa
     6961 ccgggagcaa ccgcgtgccg ggaaggatgc cggcggtgcc cggcggtggc ggctgtggtt
     7021 cacagtgaag gaacaaaacg gcggggattt cgagagccga ggacgaggga cgcgagccgg
     7081 gagccgaact caggaggagc cgttgccgtt ggcgatgtag cggaggccct ggaggaggtg
     7141 ggtatcctgc aggtcgtgga tcagggcggt gcagggggcg atgccgaaac cgggtccggc
     7201 ctcgacgcgg acctcgtcgc cgttgtcgcc gatgacgaac cacagcttgc gccgtccgcg
     7261 gtgccgggcg gcgatttcgc tcaggcgggt caggttggct tcggtcacct gctgccaggt
     7321 gagctggacc tcgagcgtgc ggatgcgctc ggcgcggagc tgatcgatgg gcttgccggc
     7381 gtggacgcgg atcttgatgt tgtcgcgcca ggaatcgacc ttgccgcgga cccagatgac
     7441 ctcgccctcg cggattgcct cgcggcaggc gtcgaacgcg tcggcgaaga aggtgcattc
     7501 catcgggccg gtcatgtcct cgcattcgac gaaggccatc tggcggccgt tcttgtccat
     7561 gcgggtcacg atgcgcttga tcaggccgac ccactcgacg tcgtcgccgg gtttcatcgc
     7621 gaggatctcg cgggcggagg cggtggagaa ggcggcgaaa tcgggggcga agcgctcgag
     7681 cggatggccg gtcaagtaga agccgacgag ttccttctcg gtttcgagcc gttccttgtc
     7741 gccccagtcg ggcaggcgcg ggatcggcaa ttcggcgcgc aggccggcgg tgtgttcctc
     7801 ggtcatcatg tcgaagatcg aggtttgcgg atcatcggca tcgcggcggg cggtaccggc
     7861 gacttccatc agccggggca ggacggcgag caggctgggg cggttgtggc cgaaggcatc
     7921 gaaggcgccg caactgatga gggcctcgac gacgcgcgag ttgatcaggc gcttatcgac
     7981 gcgggtgatg aaatcctgga gcgaagtgaa gggtccgttg gcagagcgct cctcgatcat
     8041 ggcgcggacg gcggattcgc cgacgccctt gatcgcggcc aggccgaagc ggatgttgcc
     8101 gccgtcgggg gtgaagtgca cgccggagct gttgatgtcg ggggggagga tgcggatgcc
     8161 ttcgtcgcgt gcctcctcga agtacatgcc gagcttggcg tcgtcgccgc cgacttcatt
     8221 ggtcatcagc gcggcgtaga agtcgacggg gtagtgcgcc ttgagccagg cggtctggac
     8281 ggtgatgacg gcgtaggcgg cggcgtggct cttgttgaag ccgtattcgg cgaatttctc
     8341 catggtggag aagatccgct cggcggtcgg ctcgtcgatg ccgcggctgc gggcgccgtc
     8401 gatgaaggtc cggcgctgct tggccatctc ctcctttttt ttcttgccca tggcgcggcg
     8461 gaggaggtcg gcgccgccga gcgagtagcc ggcaaggacc tgcgcgacct gcatgacctg
     8521 ctcctggtag acgaacaggc cataggtttc cttgaggatc ggctccatgt cggggtgatc
     8581 gtaggtgatc tcgcggcggc cctccttgcg ggcgatgaat tcggggatgt tgtccatcgg
     8641 gccggggcga tagagcgaga tgagggcgac gaggtcggag aagcgggtcg gtttgaaatt
     8701 gaggaggagg tcgcgcatgc cgctcgattc aagctggaag acgcccaggc ccttgccctg
     8761 ctggaggaga cggtaggtgg cctcgtcgtc gagcgggatg tcggcggcgg tgatggtccg
     8821 gccgagacgg gtgctgattc ccttgaggca gcggtcgatg atcgtcaggt ttttcaggcc
     8881 gaggaagtcc atcttgagca ggccgatttc ctcgacctcg gtcatggtga actgggtgac
     8941 ggccatggcg tcggggtcgt tatccgacgg gcggtaaagg ggaatcaggt cgatgagcgg
     9001 ctggtcgcag atgacgacgc cggcggcgtg ggtgccgggc tggcggatcg cgccctcgat
     9061 gccgcgggcg cggtcgagca gctcacgcac ctcggggtcg tcgttgtaga gcgtctggag
     9121 ctcgggcgaa gccagctcgg gatgcctgga gtccttgccc agcgcggcgt cgatggtgac
     9181 cttcggcccg ccggggatga gcttggccac ctggtcgacc ttgggcaggg cgacgcccat
     9241 gacgcggccg acgtcgcgca ccgcgttgcg ggccttgatc gtgccgaagg tgatgatctg
     9301 ggcgacgcaa tcctggccat aggtgcggcg gacgtaatcg atgacctcgc cgcggcgttc
     9361 gaagcagaag tcgatgtcga agtcgggcat cgagacccgt tcggggttga ggaagcgctc
     9421 gaagaggagg ccgtgctcga ggggatcgag atcggtgatc tcgagggcgt aggcgacgat
     9481 ggagccggcg cccgagccgc ggccggggcc gacaggaatt ccctgatcct tggcccagcg
     9541 gatgaaatcc cagacgacga ggaagtaatc gacgaagccc atcttttcga tgacgccgag
     9601 ttcgaagtcg gcctggcggc ggtggcgttc ctcggggcgg ccgccgtagc gcttgtcgag
     9661 tccgcgctgg acgagcaggc gcaggtaggt cgggccgtcc atgccgtcgg gggtgcggta
     9721 gcggggcagg agcttctggt tggtggggat gacacagttg cagcgctcgg cgatggcgac
     9781 ggtgttttcg atcgcgccat tgaggtggcc gaaaagcgaa cgcatctggt cgggcgattt
     9841 gaggtagaac tcattctgct cgaaccgcat ccgctgggga tcgttgagcg tcttgcccat
     9901 cgagatgcac atcagcacgt cctgcgagtc gtgcgccgcg gcggtcaggt agtgacagtc
     9961 gttggtggcg atcagcggca ggtcgttctt tttggccagc tccagcaggc cgttgttgac
    10021 ggtcttctgg tcgggcatgc cgtggtccat cagttcgacg aagaaatggc cgcggccgaa
    10081 gatctgggcg aagacgtcga tctggcgctg ggcctcgcgc aggtcgccgt tgagaatcgc
    10141 ctggcagacg ggcccctgaa tgcaggtggt cgtgccgatc aggccgtcgt gatagcgctc
    10201 gaggagctcc agatcgatcc gcggcttgta gtagtagccc ttgaggaagc cgagcgaact
    10261 gaggcgggag aggttgcggt aaccctcgac cgactcgcag aggaggagga aatgatagcg
    10321 ggtgcgggcc ttgttggagg tgcggtcggt gtgggaggtg ggactgaagt aggcctcgca
    10381 gccgatgatc ggcttaacgc cgccctcctg ggcggccttc tggaaggcgt aggcgccgaa
    10441 gaggttgccg tgatcggtca gggcgacggc gcccatgccc aactgcttcg tctgctcgat
    10501 gaggcccttg agactgttgg cgccgtcgag ggtgctgtaa tggctgtgga cgtgcaggtg
    10561 gacgaagggt cccaggtcgg ccatggtgag ggcgctccgg atcgccgcgg cgggctgctg
    10621 gacctatgaa agccggttgg cgggcggggt gtcaagggga agagggggcg ggggccttga
    10681 cttgttaagt attggtttta ttgaatttat gattttataa cgcagcgtcg gcgggggctt
    10741 gaagtgcgcg ggggtcgggg cgcggagggg tgtatccccc gtgaactcaa gggttcgggt
    10801 ggggggtgat ggaagggttt gggggtggcg aatgatgggg gaaaattttg ggatggaggg
    10861 gagaagattt cgagagccga ggacgagaga cgagagccgg gagccgaatt ccgggtaggc
    10921 cgttgggggc gatgacgagg aggacggacg agctgtgttg agggggactc agacggcgcc
    10981 gaggtcgcgc agggcctgtt cgagttccgc gttgcagcgg gcgtgccact ggcgggagtt
    11041 ttcgccgggc cgccgctcgg ggagggggcg gggcttgccg aagacgatcc gcagcttgcg
    11101 cggccgggga aagagctgtc cgcggcgcat gatgtgatag gtcgactgca tctggacggg
    11161 aatccagggg acgccgggga ggtcatcgag gatgaggatc cacccgcgct gaaggggctg
    11221 catggtcccg gtgggggtgc gctggccctc gggaaagtag agcaggggcc agccggcgcg
    11281 caggatccgt cggcagaggc ggagcgcctc gcggtcgccg ccgccgcgcc ggatcggcac
    11341 gccatggtag aagcggcggc agagccagcc gagcaggggg acctcgagga tttcggcgcg
    11401 cgagagggcg tagatctcgc ggttgagcgc ggcgcccacc atcggcggat cgatgaagct
    11461 ggaatgggtc ggcaggatca gcgccgggcc gctggggggg aagtgctcca ggccgatgac
    11521 ctcgcgccgg caatagagcc gggcgacggt ggacatcacg aagtgggtga accggtagaa
    11581 accgcgcagg ccgggatgcc agggctccac ggcgggcagg cgcgccggcg cttgcccggg
    11641 cttggtgagt tgcgatgcgc tcatgggtgc gaggggggct ccgcggggga tcggggattg
    11701 ggggcgcgca agcgcgcgag caatcgaccg agcaggcggg ggcgccgcgc gccgccgtca
    11761 gcttcgcccg gctgggccca ggcggtgagg tagcgggcga tccactggcg cggatcgctg
    11821 gggtcggggg ccgggggggc ggcgggggat gcccgcgcca cccagtcaag aaacccggcg
    11881 caggtgtgct cgaagagata ctggcggcgg cagaactgac ggccggcctc gcccagccgg
    11941 cgacgctcgg cgtcgttctc gagcaggcgg aggacttcgc cggccagcgc gtcgggattg
    12001 gcggagggga acggggcaag cgcgccggcg gcggccatct ccgcgacgac ctcggagccg
    12061 gtcgaagcga cgacggggca gccgaccgcc gcgtgataga gcagccggtt gcgcgcgccg
    12121 aggtgggttt cggcgcaggg gcggtcggtg agcagggcca cgtcggcgag cgccagccag
    12181 cggttggcct cggcgagcgg aagccagccg agggcgtgga agcgatcgcg atggggggag
    12241 gcgtcgacgc gctcgcggaa gagttcgaag agccgcgcga ggtagccggg cagggcgccg
    12301 ccggtggcga ccaggtgcag atcgggccgg cgcgccatcg cgcgctcgat gccggcgacg
    12361 agggtggccg gatcggccca ggcattgaag ccgcccgacc agagggcgac ccgggctgcg
    12421 gggggcaagc cggcggacgc aaaggcgcgg gcgcgctcgt cggcggacgg cggcggccgg
    12481 tcgtcgagcg actccaggca gcagggcatc ggttcaatgg gcgggcccgc cggatgccgg
    12541 gcctccggcg ccgcggcgag gcggccggcg agcgcgagct ggccgaggag cgcgtcgcgc
    12601 tggcggcgcg agacgacgga gaaggcatcg gcgcgcaaca gggtccggag catcagctcc
    12661 cagacgagca gggtcgaatc gagccgctcc gggccggggg ggaggaactc gttgcgggcg
    12721 tggatttcgg cgagggggtc gccgaagaaa tcggcccaga cgggggcgtc ggcagccagg
    12781 gcgcaggcgc gcgcggcggc ttgcagcgtg ccggcgccga cgacgcgctc gggcgcccag
    12841 gccgcgacga ggctggggat cgccggatga gacttgagat cctcgaccgt aagggcgagg
    12901 tggcgccagc gcagcgcgcc gcgcgatcgt tcctcgggcc gcggctcgtc gcgatcgagc
    12961 gccgtggcgt ccggttcgat cgtcaggacg agcgcttcgt ggccggcctt gagggccggc
    13021 aggaggaagt ggagcaagcg attggcgacg ccgggagcgt gcagcgagcc gccgtccgga
    13081 accggtccgg cccccagcag gaggagtcgg cgcggggggt gcgtcatggg gcggtagaat
    13141 ggggcatggt cggtggtttt ccgggacaga tgggagcagt tggaatcccg cgggtttgag
    13201 gttcgttccc ggaatccttt ttacatcttc cggcggcgga cttaaactgg acgatccgtg
    13261 ttccgcggat gatcgaccga acaggccaac ggggcgccgc gggcgggggc gttcagcggc
    13321 ccaccatacg actggaagcc cccagaacac aacaggaagt gaggcgcgcg gcggcgtggc
    13381 ccgagcatga tacggcagca tcacacgacc caccggcaca cgctgggctg cggcatgccg
    13441 ctgctcctgc tggagaatca cgaggcggcg gtggcgacgg ccgacatctg ggtgcggacc
    13501 ggggcggcgg atgagccgga ggatttaagc ggcatttcgc atttcctgga gcacatgctg
    13561 ttcaagggga ccgagaagtg ggggctgggg gagatcgagc gggcgatcga atcggtgggg
    13621 ggcgtgagca acgccgggac gagctacgat ttcacgcact attacgtgac gctgccggcc
    13681 gcgcggatcc ggacggggat cgagatgctg gccgagatgg tgcgccactc cacgctggac
    13741 gcggcggaac tggagaagga gcgtctggtg attctggagg agtatcggcg caagcaggac
    13801 aacccgatcg gcgtgctgta tgagctgctc tatggggaac tgttcgaggc ggggccgtat
    13861 catctgccgg tgatcggcag cgaggaaacg atccgcgcga tcgaccggcc ggcgatgctg
    13921 gactactatc accggcgcta cgggccggag ggggcggtgc tggtcgtggc cggggacatc
    13981 gaaccggagc gggcgatcga gtgggcggag gccgcgtttg ccgggttcga ccgccgccgc
    14041 gaggaactga cgcgccgggc tccggtgcgc tacgggcggg gcaagcgggt gcatgagcgc
    14101 aaaccgacgg ggggcgaggt gtatgtggcg tatgccttcc cggcgccggg gatgcaggcg
    14161 accgaggagg tgctgccgct cgacatggcg cagttcatcc tggggagcgg gcgcgccgcg
    14221 cggctgcacc aggagctcaa ggagaagcgc gggctctgct cgtcgatcag ttcgtattat
    14281 ccgacgcatg cgcacgattc gctgttcgtg ttcgtggcga cgtgccagcc gggccagcgg
    14341 gaggggctgc gggcggcgct gctggaggaa ctgcggcgat tcgcggcgga gccgccgacg
    14401 ccgccggcga tgaaacgggc gcggcggctg ctggccagct cgcacctgtt ctcgatggaa
    14461 acgagcagcg gcgccgcgag caacatcggc tattactaca cgctgagcgg ttcgaccgag
    14521 tttttcgatt cgtatctgga gaagctggag gcgttgacgg cggagcaggt cagtgcggcg
    14581 acggcgcggg tgatccatcc cgagcggctg gaggagagcc tggtggagat ttccgtcggt
    14641 cccaacggcg agctgaaggg ggaaggctga tggaagcggc gcacgggggg tcgtcgggtc
    14701 cggtgcggtt cgaacggatg aagatcgtcg gggccgacgc gctgctggcg ccgcggccgg
    14761 ggacgggcct gctggcgctg gcggcgatca tccggcgggg ttcggccgat gagcgcgagg
    14821 gggaacacgg gctggcgtcg ttcaccgcgt cgatgctgct gcgcgggacg cagcggcgca
    14881 gttcggaaca gatcgcgttc gatctggagt cgctcggcgc gatcgcgggc gaaggggacg
    14941 ggatggacac gtgcagcctg aacgtgcgct gcagcacgga ggcggcgccg cgcgcgctgg
    15001 agatcctgtt cgagtcgctg cgggagccgg cgttcgacgc gcgggaacac gaggtgcatc
    15061 ggcaggagtt gctcgcgcac ctgcggatga tcgaggatga gaaattcagc tatacctatc
    15121 gcgaatacgt caaggcgatg ttcacggggc acggatacgg ccacgccagc gagggcgaac
    15181 tggccgatgc cgagcggatc acgccggcgg actgccggcg ctggcatgcg gagatgatcc
    15241 ggccggagac actgctgatc gcggcggtgg gggattttga ggtgggggcg ctgcgcgatc
    15301 agttggcggg gttgctcgcc gactggccgg tgaacgacgg gccggtgcgg ccgcggatcg
    15361 cgacgcagcc gccggccgac cacccgcgcg agctggaact gcgcaagccg gagctgaacc
    15421 agggcttcat cgtcggcggg ttccggacgc cgccgatcac cgatccggat tatgcggcgc
    15481 tgcggctggg gtcggccgcg cttggcgaag ggttcgcggg gcggattttc acggagctgc
    15541 gggagaagcg ctcgctggcc tatgcgctgg gggcgacgct gcgttcctat cggctgacgg
    15601 ggcatcaggt actctacatc gggacgaagc ctgaatcgat cgaagaggcg cgcgcgggcc
    15661 tgctgcgcga ggcggactat ttgaagcaca acctgctgag cgacgaggag ctgaaccgcg
    15721 cccgcgagta tgtgctgggg aaattcgtga tggggcagca gtcccacgcc cagcgggtca
    15781 gccacctggc gtggtgggaa gacgccgcgg gcgacgccgc cgaggggtgg cggtatccgg
    15841 agcggctgag gggggtgacg gccgcccagg tgcgcgaggc ggcgcgcaag tggtgggtgg
    15901 agccgaccat ggcggtgttg cggcccgagg cgggatgagg cccggcacgg ggtgagcggc
    15961 gcgccaggcc ccgcgcggcg gccgcgacca acggaaattc aacgcaaagc acaaagacgc
    16021 gaagaagaac cggaaggctt ggcgccggcg gcgcggcggg gccgtgtcaa ttcgcgaatc
    16081 agccggggcg ggggctaagg cggacgggtc tgcgtgccga taaggccagt ggaggcgttg
    16141 caaatggcgc gactccggcc gtgggttgcg ctacgtcctc ctgatcgtga acagggatgg
    16201 ttcaggatgg gcaaccacgc agcgatcgaa gtcaatcaac ttcacattcg atatggccgg
    16261 ggggccaggc gtcacctggc cgtcgcgggg ctcacgtttc aggtcgaacc gggcgagacg
    16321 gtgggattca tcggccccaa cggcgcgggg aagagctcca cgatcaagac gctgatgggc
    16381 tttcagtatc cgaccgaggg cacggtgcgg ctgttcgggg agcaagccgg gagcatcgcg
    16441 gcgcggcggc ggatcgggta tctgcccgag gtttcgctgt actatccgtt catgaaggcg
    16501 cgcgaggtgc ttgagctcta cggcgggctg caggggctgg accgcgcgac gctcaagcgg
    16561 cggattcccg agttgctcga gcgggttggc ctggcgggca agggggagac gctactgaag
    16621 catttcagca aggggatgca gcaacgggtg gggatcgccc aggcgatcat cgccgacccg
    16681 gatctgttga tcttcgatga gctttcgtcg gggctggacc cggtgggccg gtatgatctg
    16741 cgcgaggtgc tgctcgaatt gaagcggcgt aaccggacga ttttcttctc gtcgcacgag
    16801 ctgtacgaag tcgagtcgct gtgcgaccgg gtgatcatga tccacggcgg gcggaaggtc
    16861 gccgacgcgg ccgtgccgga gctgatggag cagctcaagg cgggcaacgg cggcaggggg
    16921 cagtcgctgg aggagttctt catggggctg atccgtcccg cggaggccgc gtgatgttgc
    16981 gccgccagtg gttgattgcc cgcacgatat tcgtggaagc gctgcgccgg cgcgagatct
    17041 acgccatcgt gctgatcact gccgggatgc tgctgctggc ctcgctgctg cgcttcttcg
    17101 acctgcaggg actgcacaag ttctatcacg aagtatcgct gaaggtgatg acgacggcga
    17161 cggtgctgac ggtgatcgtg ctggcggcgc ggcagcttcc gcgcgagttc gaacggcgga
    17221 cgatctacac gctgctggcc aagccgatcg cgcggtggga attcctgctg ggcaaatacg
    17281 tcggcgtcgt cctggcgggg ctgttctgcc tcgggctgtt catgggggtg ttctgcctgg
    17341 ggcggctgtt cacgcaggcg ccgctggact ggcggctgtt cggccagttc ctgttcatgc
    17401 aggggctggc gatcgggtgc ctggccgcgc tctcgtttct gctttcgatg ctgatggggc
    17461 tggatgccgc gatcgtcacg gcgatgctga tctacctgct ggggcaggtg atgaccaacg
    17521 cgatgacggt ggtatacgag tttaccgggg cggcggggcg cgcggtgctg atcacgctca
    17581 actacctggt gccgcagccg gcgctgttcg atttgtcggc gaaggtggtg catgagtggc
    17641 cgccggtgcc ggcgtgggtg ttggggctgg ccacgctcta ctgcctgatg ttcgtgatcc
    17701 cgtatctggg gctaagctac gcgctgttcc gccgccgcgc gctgtaaatc cgaggaaagg
    17761 ggaagcatca tgagtctggc cgcccaggaa accctgcttt ccatggaaac gccgcggggg
    17821 ccgcgcccgt gggggccgct ggcgcgcgcc gcctggattc aggccgtgcg gcgcagcgag
    17881 atccacgtgc tgctgattct gctggggctc tacggcctcg ccgcgctgat tttgcggctg
    17941 atcgggattg agtcggcgca gacggcgcga ttcatcgccg ggctggggct gcaactgggc
    18001 agttgcctgg cgatgctgct cgtcatcatc ctgatcggcc ggcaggtgcc gaccgaaatc
    18061 gaccagcgga caatttatcc gatgctggcc aagccggtct cgcggatgca gcttctgctg
    18121 ggcaaggcgc tgccgatctg gctggccggc gtcgccgccc tcgggctgtt cgtgggggtg
    18181 acgctgctga tcacgccgcg gctgccgatt cagcacccgg tcgtgctggc ggaggcgttg
    18241 gtgctgcgcg cgggagggct ggcgatggcg ggggcgctgg cgatgtggct gtcgctgcgg
    18301 ctgccgatgg cggtggcgat gctggcgacg gcggcggcgg cgttcttcgg cgggatggcg
    18361 ggcaatttcg cggcgcaggc gggcgggcgc ctgggggcgc tggcggcccg gttcattccc
    18421 gacttctcgc tgctggatca gtttcagcgc tatgtcgacg gcggacggcc gctgagcctc
    18481 gccgaaatcg cggggctgct gctctacgcg gcgctgtgga cgttcctctt ttcggtgctc
    18541 gcgggaacga ccttccgccg gatggccttg taggagtgcg tctcatggag cggttgaacc
    18601 aggcaccgcg gccgggcggc ggatattcga tgctcggcac gctgacgctg cttgccggtc
    18661 tcggcgcgat cgggctgttg cggccggcgc tcgagccggc gaaggcgtgg cagccggcct
    18721 tcgtgatgac cgaggcggtc gccgagcagg tcaacaccag cccgtttgcc ctgattctgg
    18781 gggaaatccg cgcgtccgcg gccgacctga tgtgggtcaa gacggagcgc tacctgcacc
    18841 gcggggtggc ctacgcgccg cacctgaatg ccgacaacct ggcgcggacg ggcgagacgg
    18901 tcaatcgggc agaagagagc cgctccgccg gcgcggagca tgtccatgac gaacattgcg
    18961 accatgaagc cgatgcggac ggggccggcg cagatgcggg ccacggggca gaggtggcgc
    19021 tgatccccga tcgcgagtcg gactatcgcg gtttcatcgg cgtgctgcat cgcgaggtgc
    19081 agccgtggga gggaggggat caaccgcatg agcattcggg cggcgagcag cttctgccgt
    19141 ggtaccggtt gctgacgctc agcgatccgc atcactggcg ggggttcatg atcggggcgt
    19201 ggtggctgat gcagcagcgc gaggagggcg ccgtgtcgct ggccgaggcg cggagcttca
    19261 tcgaggaggg gatccgtcac aaccccgacg tgttccaact gcacctgatg cacggccgcg
    19321 tgctgacgcg gctcgaggaa tgggacggcg cgatcggcgc gttccggcgg gcggtcgagg
    19381 tggggttgaa gctgcgcccc gaaggcggag tcgaggagcc gccggtctgg acgctgtcgg
    19441 aggaggagga cttcgagatg gccgtgcggt atatcgcgat gattcaggca cgcaagttga
    19501 acgatatccc ggccgcgctg gcgacgattg aatggggcct gcggctggtg ccgggcgacg
    19561 ggccgctgac gcacctgcgg gaggatctga cgaccgccgg ggaatgaggg ggggacgatt
    19621 ttcggcggtc gttcgcgggg gggggcagtt gggatttggg gccgcggtgg gggttgaggt
    19681 tggggtagcc gggttctgac gtccctgacg ggactgcgtt ggcattttcc attggcattt
    19741 actgttttca ccccatgatt caggcgagcg cgggaggcgc cggcgtcgcc ggcctcctct
    19801 tacgggagtt tggggcgttg gggcgtgacg cagggttccg gactgcggcc gtcaccctgc
    19861 gctttaatcc ggtcgtcggc tgcgcggact gaggcattcg ccccctgcgg ggacggggat
    19921 ggtgtggaat gcggtggccc caggatgcgc tgcgcgccac caggtgttta aacagcgctt
    19981 cttcgaggcg gcacctggca tccctgccgg gatgcggggg gatggaatgg ctgggggacc
    20041 ggtggtgtcg ctgtactcaa ccaccgggta ccctcaatca tcccttcggg atgggggatg
    20101 ggtgggagtg cgggttccac accggggggg cggggcgctc gacatctctc atgatcgatc
    20161 ggcgggtcag aacggaaaat tatggcgggt tgtcgcgagc
//
LOCUS       DAOPCO010000040        20085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2806_length_20085_cov_5.660559, whole genome shotgun sequence.
ACCESSION   DAOPCO010000040 DAOPCO010000000
VERSION     DAOPCO010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 20085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20085
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_20085_cov_5.660559"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLS90_04965"
     CDS             complement(<1..1243)
                     /locus_tag="PLS90_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK01788.1"
                     /translation="MAEKPLRIALHCLLLESPAIDRRQLEDALQASYPDSSVTPRDSP
                     RAAQEALRVSVYDLVLLCLAPLSDEHGALLEALLAHPTPMPAILIIAPGTEEALFELM
                     PRLPGEWEVLDSSQLTVTGLHRRIQKAIETHQQQWALRHLQSAFQSSLARYRNLFDEV
                     PDLIFICDRSGCLLDVNSTAHRLFGRDKEEILMRPIFEVFGMAREDFDRLLERALAQD
                     GPIQDIEIKYHHPSGQIIYGLTHLIRWQSDGGRPLQFQGVIKDISPHKRLEQQLRQSE
                     DKYKTLYDLARITVSSLKLEEVVDRSLNLIHSCCQAKGALLLLNRQEDELVLVGATAG
                     HEILAREGVDEALPRVGQGVLGRLAIGRGLHVLERDELAELPPRLRDWLAENEPEQVV
                     AITLGHNNPTLPASLLLMALSE"
     gene            complement(1610..2839)
                     /locus_tag="PLS90_04970"
     CDS             complement(1610..2839)
                     /locus_tag="PLS90_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01789.1"
                     /translation="MMPQPELSARAAAASIRVAHLLDGRHFGGAEQMVRRLALASPRV
                     GVAASVYCLSQGRLSEALRADGIPHQVFRSAGRFDLTPLVAMTRTARRDRIQILQAHT
                     SRTHLMARLLSRRLGIPNVTQIQSPIALDENQAHGRHPLRAWVERLGRPWTDAIVTVC
                     EEEARRLVREEGAPPARVRSIPNGLDAPDEPDRTAARARMIQALRERHGVEVGPGEFV
                     VAMIAQMRPRKGPEVLLRAFAQWRRRGEPGRLLIIGDDEFVERGGYLARLKALAAELG
                     LGDHTVFTGFVAEPWELAAGASLFALPSLFGEGLPLVLVEALSRGLPLAVSDIPGNRE
                     TVRPGETGWLHAPGEHDQLAEALQAASRNPEQAERMGRGGRRLFLERYTMDAVLRRFR
                     ALYEDLLARDRGRHPGS"
     gene            complement(2836..3960)
                     /locus_tag="PLS90_04975"
     CDS             complement(2836..3960)
                     /locus_tag="PLS90_04975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01790.1"
                     /translation="MTGRILILSFVEWSGAFQRPQHLAVGLARRGWEVTYASPGYLHR
                     RGRGAAPGVDLPPSLRILSPAAVPGGSRLRIAAEVNRRLMAAALNRESPQGWETIIFN
                     DPRWAGVAAALPARMRVFDCMDDLTAQAPSAEWARAREDEALRVADRIWTGTASLEAR
                     LRDQAAERGKPIRFVPCGVDADHFASPDARRVAALRPMLPPGAGPLAGYFGALNERVR
                     GELVEALLAGGARALLIGPTTSRAPAIVKQAGRHPGLRWTGPRPYAELPAWLALFDFA
                     IIPYDTGGPHRFLYPVKALEYLAGGKPVLSTPLPDVLRFLGDYVEIGSTAEDWRAAPG
                     RIRAETESIRKKTERGRDYARSRSWEAMVEEMLADLTRDQ"
     gene            complement(3984..5189)
                     /locus_tag="PLS90_04980"
     CDS             complement(3984..5189)
                     /locus_tag="PLS90_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK01791.1"
                     /translation="MQIAVDARFLGSANSGVARYSENLLVAMSRIDRANQYTVFVHHR
                     LKRKLKLGENFTIVRVKGHPLSIRSMWQLSRMVRPEAFDLLHVLFPYVSIRARCPVML
                     TVHDTLPFSREQIGFGPKRGPQSWLRRYVLYPLALRRAKWIICVSRATRDALAELFPE
                     TFHRTLVVHSGVNEDFRQAAEEATLELIRSRLNLPPRYLLYSGSARSDKNIEGMLRAF
                     ARLRQLNDSMDDLHFLMDIQGHEYPLKPIMNRIAQYGLEGRVRIIQDIGDEERVVLFQ
                     HAAAFLILSRTEGFGIPILEAQMCGVPVVAADAMALPEVAGEQGALYVDPDDLDETVR
                     LLGLALTDDGLREYLIAEGRKNANRFSWARAAEQIVQIYEFGFYPRHQVELPHRRPWL
                     TRLADALRI"
     gene            complement(5257..5538)
                     /locus_tag="PLS90_04985"
     CDS             complement(5257..5538)
                     /locus_tag="PLS90_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPK01792.1"
                     /translation="MRLSEDRINAIAYKIAFELVKKRYVKTSRRLSQVTAWVEKPILD
                     NLRQEEALDEEVRRTLAGMKTAPPEGSFEYQALYQRKKEELARRRGYQL"
     gene            complement(5541..5822)
                     /locus_tag="PLS90_04990"
     CDS             complement(5541..5822)
                     /locus_tag="PLS90_04990"
                     /inference="COORDINATES: protein motif:HMM:NF016271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HPK01793.1"
                     /translation="MRLRPEKIEHLANLVFESLSQNPELTLHESRDHIVGLVRRVITD
                     DLRMEDEIEEEARRVLESHRGEIDRTGASFEKLVLKAKQKLAQERKMVL"
     gene            complement(6034..6109)
                     /locus_tag="PLS90_04995"
     tRNA            complement(6034..6109)
                     /locus_tag="PLS90_04995"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6074..6076),aa:Phe,seq:gaa)
     gene            complement(6351..6427)
                     /locus_tag="PLS90_05000"
     tRNA            complement(6351..6427)
                     /locus_tag="PLS90_05000"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6391..6393),aa:Arg,seq:acg)
     gene            complement(6674..7426)
                     /locus_tag="PLS90_05005"
     CDS             complement(6674..7426)
                     /locus_tag="PLS90_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK01794.1"
                     /translation="MSTDPPRRIAVFSDIHGNLHALRAVLDDIDRLGLRDLVCCGDVV
                     GYGAFPNACCDILRERGIPTLAGNHDHAAIGMTDVSFFNEIARAAVQWTADELTDENR
                     HWLRERPYTYCLDPDFFFVHASPLKPEQWGYVLTFGDARLGFNEFTQRCCFIGHSHQP
                     AIVIQQGADLSCPDENQPVIRIRTGERYLINVGSVGQPRDHNPQACYVVADLDEGRIS
                     YHRVPYDIEAAQQAIVEGGLPQVLAERIAFGW"
     gene            complement(7416..8987)
                     /locus_tag="PLS90_05010"
     CDS             complement(7416..8987)
                     /locus_tag="PLS90_05010"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPK01795.1"
                     /translation="MPEIIVKLGDNIVQNHLFTNERITIGRAPENDIVIENLAVSRNH
                     AVITWENDRFYLEDLGSSNGSFINGVKVKRNEVVDRDVITIGKHKIYFYDRMTREPVR
                     RAQPAIVEGTMIVQQSMVAELVVTKGRQKGQIFRLDGNSITLGRGGQNDIRLTDWFVS
                     KNHAVIEKRGLDYYLVDLGSWRHTMLNGEVVTEAILNDGDVIQLGPTVQLGFQLQEHL
                     EQMRPGDEAELDDDGNDADSVEDELDRLSESMQNEATRVLRKPVVPPETPPETPSASV
                     EEEELDLEEELDELEHVRGLNGCANGSAAPGAATSPSPAEIDETLGAHDELDPPPADE
                     ILGAHDELDLPPADEILGAHDELDLPPAGDESPAPPVAEAYEVPPPSPAELALEQDEW
                     EELETDEPLVAPLPSTGCAQEIAEATAIIPEQDPVAEMTAQPDLLAEAAEEFDHEPEP
                     AAAEPPVAAAPTVDPLPADADVEREPAGADEPADAKLQDEVRMWERALTNKSPVIRKQ
                     AARMLKKLTGKDYEY"
     gene            9259..10176
                     /locus_tag="PLS90_05015"
     CDS             9259..10176
                     /locus_tag="PLS90_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01796.1"
                     /translation="MNLTPLNPEHLEEIADWWRRDQRLHPLAPGLLRERLFPPEAHEP
                     DLLIGLRDERERLVGLAAGEFPARWGESTGGVRWLGLSPPALAGGGLGPLLGELERRL
                     TARGARKLYLHATPPYYIRPGVDTRDTALIVALLELGWRHEETHFNLTVDLAAWAPPE
                     ASAIWGSPADAAAVRRATPADATALASLIEQHWSPGWRRESALGFGHDPISVFVAETA
                     GGLVGFSAYEVSQCDGSFGPTGVVAGQRGQGLGRRLLWACLSDLKRLGRSRCEIGWVG
                     PVPFYHRACGATLGPTFWMMTRELAQPGN"
     gene            10186..10851
                     /locus_tag="PLS90_05020"
     CDS             10186..10851
                     /locus_tag="PLS90_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPK01797.1"
                     /translation="MPPEITEFKALGKRRQRIMVYLDGAPWAELDPETVVRERLSRGQ
                     NLDEAARAALLRADEQIRARKAAAGHAAGGRKTRHELEQYLRERRFSPRAIAGALQAL
                     AESETIDDEQVAGRHIRRRRRGRDLGPRRLEAELVARGIEPRQAESLVDKTFSEVDVR
                     RECRELAARAAARYQPLTEPKQRRRLAAWLLRRGYGQEDVESALEWLRTEVQETGREP
                     TPD"
     gene            11101..11622
                     /locus_tag="PLS90_05025"
     CDS             11101..11622
                     /locus_tag="PLS90_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPK01798.1"
                     /translation="MRIHAMCRNARSGKGATIMTLNYWDPMREFANLRRELDRFFERT
                     DNYWTFPFTRFSFLPGRAARAYPLLNISEDADNVYIEALAPGVDPQSLKVEVIRDQLS
                     ISGEKPAAVPDGKAEGYHRNERAAGRFVRTFTLPAEVEQDKVDAEYRDGLLTITLPKA
                     EAAKPRQITVNMK"
     gene            11688..12083
                     /locus_tag="PLS90_05030"
     CDS             11688..12083
                     /locus_tag="PLS90_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPK01799.1"
                     /translation="MAETTIATTNTELEARPTETREPSRYQIPPVDIYEHDHGLTVFA
                     DMPGVSSDRVEVNVDNDVLTIRGRVQRETKGDAVSTEFAILDYFRQFKLNQEVDQEKI
                     TAKLNHGVLTIDLPKVEAARPRQIPVSVG"
     gene            complement(12255..13556)
                     /locus_tag="PLS90_05035"
     CDS             complement(12255..13556)
                     /locus_tag="PLS90_05035"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPK01800.1"
                     /translation="MRAVDLIHAKRQGERLDAEAIRWLIGEYVRDRIPDYQLAAWLMA
                     VFFRGLDGDELAALTDAMLRSGEIFDWSGIEGVKADKHSTGGVGDKVSLALAPLAAAC
                     GLIVPMVSGRGLGHTGGTLDKLEAIPGFKVNLTRAQMRRQLARLGVAMMGQTDRFVPA
                     DKRLYALRDVTATVESIPLIAASIMSKKLAEGADVLVLDIKVGSGAFMKTTREARLLG
                     RKMIDIGRKMNRRVAALLTDMNQPLGRAVGHSNEVCEAIDLLRGGGPPDLKEVTWALT
                     ARMLTLAGLARDAAAARERLDAAVASGAALDRFRRLIEWQQGDPRVVDDARRLPWARR
                     EAVVPAPATGWLTRIDTRQIGLALAALGGGRRTKEDRIDYGVGIDVERRLGDRVERGE
                     PLFRVHYRKQRDWETARRMLTAAVTISEPRKRPPRLIMEEL"
     gene            complement(13564..14292)
                     /locus_tag="PLS90_05040"
     CDS             complement(13564..14292)
                     /locus_tag="PLS90_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01801.1"
                     /translation="MLPPPMTFPRLLPACCCLAAALILAGCAGPRMKRLSDERYPPEH
                     PDTEIGVYIGELDRELDEIAIIESDAYAFVDDEIKRRQIEQLQAKARKLGANAVQDVR
                     ILAKVIKGFIADEAVPFTAWKQGEYELFFMRGVAVRAADVEPADFAELNPRGGWVVDA
                     LPVPPRLDEVIAQIPIEQAPPADAAPPAVPAADDAGEPAMIVIEPPTDTEPEVVAPDA
                     EPVGAMPVPAAPDPLPSENPILTD"
     gene            complement(14407..15621)
                     /locus_tag="PLS90_05045"
     CDS             complement(14407..15621)
                     /locus_tag="PLS90_05045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPK01802.1"
                     /translation="MTSRPPLSRDRRLLFITWAVFLPVLVVAVALAIWALPLLRERRY
                     LRYVIQTRLADQTLAGVGRAAIAPEDYADWADHVRQCYREPSAVDLERISWNLRCRPM
                     PFIGYAPEPGDQTEGAINAHQLRSRRPLVLPKPPGVFRVFLTGGSLAYSVGAPALEST
                     IAGCLETALAQRPALAGWRVEVFNAAVPAWSSTHERIRIENDISRWEPDLVISLTGVN
                     DAHWGFEGMDILSFRANEEIMYFELIRGGMRIAGAEPFAHAPPAMWGDPVDPGLVARR
                     FAWNMSLAGDALRRAGVPFLVAIQPYLAAQYKPLTPGETSWLARHEDPGKMDYIDQAA
                     DAMLQALRAEAQTAAPPPFHIADLRRVFAQRDDPIFLDGLHVGDKGNALIAAALAEQV
                     VAILTEESFGGG"
     gene            15869..17323
                     /gene="proS"
                     /locus_tag="PLS90_05050"
     CDS             15869..17323
                     /gene="proS"
                     /locus_tag="PLS90_05050"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPK01803.1"
                     /translation="MAKKKAFEDALPARSENFSEWYVDVVRKAELADYSDIRGCMIIR
                     PYGYTLWENVRDALDRRIKQTGHVNAYFPLFIPESYLMKEAEHVEGFAPEVPWVTHVG
                     EEKLEERLAIRPTSETIICATYAKWIQSYRDLPLLINQWANVVRWEKRTRLFLRTTEF
                     LWQEGHTCHRTEEEAVVETLKMLKVYHDFLREDLALPTIMGKKTEAEKFAGAVDTYCV
                     EALMGDGWALQAGTSHMLGQNFAKAFNIKFLDEDNTEKFVWQTSWGVSTRLVGGLIMG
                     QGDDAGLRLPPRVAPIQVVVVPIIFEDSKEQVLAAARELTARLETAGFRVRLDDREWH
                     NAGFKRHDWELRGVPLRVEVGPKDLAQNQAFAARRDTGEKEAIGLDGLPARVGELLDA
                     IQRTMLRQAEEFLAGHTTEVGSYEEFKRVLDEGKGLIRAPWDGDPATEARVKEETKAT
                     IRCIPLEGGEAPAGATDLVSGRPARHLVLYARAY"
     gene            17985..18440
                     /locus_tag="PLS90_05055"
     CDS             17985..18440
                     /locus_tag="PLS90_05055"
                     /inference="COORDINATES: protein motif:HMM:NF016184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HPK01804.1"
                     /translation="MKNRKVLNHQVTAGGQPSGDDLVYLKQHQFRSVVNLRTEGEADQ
                     TLSPDEERRLVEGLGMAYLHVPVSAQTISPELLGQFVAEFDDLPKPAYVHCESGPRAG
                     LFAMLWLGVNQGLTGEQAIARGREYGFSLDNPQLVEPVRRFLDSQERVQ"
     gene            complement(18517..19734)
                     /gene="metK"
                     /locus_tag="PLS90_05060"
     CDS             complement(18517..19734)
                     /gene="metK"
                     /locus_tag="PLS90_05060"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPK01805.1"
                     /translation="MTPRTRLFTSESVSEGHPDKVCDNISDAILDALLTEDPQSRVAC
                     ETLVTTDLVVVSAEITTQARIDYQRVARQTIEEIGYTDPAMGFCAEGAKYLQSIHTQS
                     PDIAMGVDASSDHEQGAGDQGMMFGYACNETPELMPLPIELAHKLVKRQAAVRRDGAI
                     AWLRPDAKSQVTIEYDEEWKPLRVHTVVLSTQHAPLYGGNSPTRDDLKKEQAEIRRQV
                     IEHIIDPVLPAELVDKNLIIHVNPTGRFVVGGPHGDTGLTGRKIIVDTYGGMGRHGGG
                     AFSGKDPSKVDRSAAYAARWIAKNIVAGGAAERCEVQLSYAIGVAQPLSINVNTFGTG
                     RVDEDLLIKAVRETFNLTPAGILRSLDLKRPIYRRTAAYGHFGRTEEGFPWEETQHKE
                     VFAELAGTAAAAR"
BASE COUNT         3277 a         6438 c         6976 g         3394 t
ORIGIN      
        1 cctcgctcag ggccatcaac agcagcgacg cgggcagtgt cgggttgttg tggcccagcg
       61 tgatcgcgac gacctgttcg ggctcgttct cggccagcca gtcgcgcagc cgcggcggca
      121 gttcggccag ctcatcgcgc tccagcacat gcagccccct gccgatggcg agccgcccga
      181 gcacgccctg gcccacgcgc gggagggctt catccacgcc ctcgcgcgcc aggatttcgt
      241 ggcccgccgt ggcgcccacc agcacgagtt cgtcctcctg ccggttgagc agcaacagtg
      301 cgcccttggc ctgacaacac gagtggatca ggttcagcga gcggtcgacc acctcttcca
      361 gtttcaagga cgaaacggtg atgcgcgcca gatcatagag ggtcttgtat ttgtcctcgc
      421 tctggcgcag ttgctgctcc agtcgcttgt gcggggaaat atccttgatc accccctgga
      481 actggagcgg ccgcccgccg tcggactgcc agcggatcag atgcgtcagg ccgtagatga
      541 tctggcccga ggggtgatgg tatttgattt cgatatcctg gatcggcccg tcctgggcga
      601 gcgcgcgttc gagcagccga tcgaaatcct cgcgcgccat gccgaagact tcgaaaatcg
      661 gccgcatcag gatctcttcc ttgtcgcggc cgaacagccg gtgcgctgtc gagttgacgt
      721 cgagcaggca acccgaccgg tcgcagatga agatcagatc gggcacttca tcgaacagat
      781 tgcggtagcg cgcgagcgaa ctctggaagg cgctctgcag atggcgcagc gcccactgtt
      841 gctggtgcgt ctcgatcgcc ttctggatcc gccggtgcag cccggtgacg gtcagttggc
      901 tcgaatcgag cacctcccat tcaccgggta gccgcggcat cagttcgaac agcgcttcct
      961 ccgtgcccgg cgcgatgatc agaatggccg gcatcggcgt ggggtgggcg agcagcgcct
     1021 cgagcagcgc gccgtgctcg tccgacagcg gcgccaggca gagcagcacc agatcgtaga
     1081 ccgagacgcg cagcgcttcc tgcgccgccc ggggcgaatc ccgcggcgtc accgaactgt
     1141 cgggataact cgcctgcagg gcatcctcaa gctggcgccg gtcgatggcc ggcgattcca
     1201 gcagcaggca gtgcagagcg attcgcagcg gtttttcggc catcagtggc aaccggattg
     1261 ttcacattgg gagcgtggcg ggttcacagc gaatcgatcc gggttccctc agaatgcgcg
     1321 ataggtaaga attcgtctcc ccttttcctc acatcgggca attttataca caagttgacc
     1381 cctgattgat acccgtttcg attttcagcg gccgcgtcgg ggaagtgagg cttccggggc
     1441 aggggttgag catgcgcgcc ggtttgcgcc ggagggagaa ccttggcgtt ggtttcgaga
     1501 gccgaggacg agggacggga gccgggagcc gaagtttggg gtttgccttc gggggcgatc
     1561 acgaagacga ggacgaggac gaatttctct agaggcgcgg ccgccgcgat caagatcccg
     1621 gatgccggcc gcgatcgcgg gccagcaggt cttcatacag ggcccggaag cgccgcagga
     1681 ccgcatccat cgtatagcgt tcgaggaaca ggcggcggcc gccgcgcccc atccgttccg
     1741 cttgctccgg gtttcgggag gcggcctgca gcgcctcggc cagttgatca tgttcacccg
     1801 gcgcgtgcag ccagccggtt tcgcccggcc gcacggtttc ccggttgccg gggatatccg
     1861 aaaccgccag cggaaggccg cgactgagcg cctcgaccag aacaagcggc aatccctctc
     1921 cgaacagcga ggggagggcg aacaggctgg cgccggcggc aagctcccac ggctcggcga
     1981 caaagccggt aaagacggtg tggtcgccga ggccgagctc ggcggcgagc gctttcagcc
     2041 gcgccagata ccctccgcgc tcgacgaact cgtcgtcgcc gatgatgagc agccggcctg
     2101 gttcgccgcg gcggcgccat tgcgcgaagg cccgcagcag gacttccggc cccttgcgcg
     2161 gccgcatctg agcgatcatt gcgacgacga actcgccggg gccgacctcg acgccgtggc
     2221 gttcacgcag cgcctggatc atgcgcgcgc gcgccgcggt ccggtccggc tcgtcggggg
     2281 catccaggcc gttggggatg gagcggaccc tggccggcgg ggcgccttcc tcgcggacca
     2341 gccgccgcgc ctcctcctcg cagaccgtca cgatcgcgtc ggtccagggc cggccgaggc
     2401 gctcgaccca ggcgcgcagt ggatggcgcc cgtgcgcctg attctcatcc agtgcgatgg
     2461 gggactgaat ctgcgtcacg ttggggatgc cgagccggcg cgagagcagc cgcgccatca
     2521 ggtgcgtgcg gctggtgtgg gcttgcagaa tctggatgcg gtcgcgccgg gcggtccgcg
     2581 tcatcgcgac gaggggagtc aggtcgaagc ggccggcgct gcgaaagacc tggtgcggaa
     2641 tgccgtcggc gcgcagcgcc tcgctgagcc gcccctgcga gagacagtag accgaggcgg
     2701 cgacgccgac ccgcggcgag gcgagcgcca gccggcgcac catctgctcc gcgccgccga
     2761 aatggcggcc gtccagcaga tgggccacgc gaatcgaggc cgctgccgcg cgggctgaca
     2821 gttccggttg gggcatcatt ggtcgcgcgt cagatccgcg agcatttcct ccaccatggc
     2881 ctcccaggat cgagaccggg cgtaatcgcg tccccgttcg gtttttttgc ggatgctttc
     2941 cgtttccgct cggatccggc cgggtgcggc gcgccagtcc tctgccgtcg atccgatttc
     3001 gacatagtcg cccaggaagc gcagcacgtc aggcaggggc gtgctcagga ccggcttgcc
     3061 tcccgccagg tactccaacg ccttgacggg atagagaaag cgatgcggcc cgccggtatc
     3121 ataggggatg atggcgaaat cgaacagggc gagccaggcg gggagttccg cgtagggccg
     3181 cggacccgtc cagcgcagcc cggggtgccg gccggcctgt ttcacgatcg ccggcgcgcg
     3241 cgaggtcgtc ggtccgatca ggagggcgcg tgcgccgccg gcaagcagcg cctcgaccag
     3301 ttcgccgcgc acccgctcgt tgagcgcgcc gaaatagccg gcgagcggcc cggcgcccgg
     3361 gggcagcatc ggccgcagcg ccgccacccg gcgagcgtcg ggcgatgcga aatgatcggc
     3421 atccacgccg cacgggacga aacggatcgg cttgccgcgc tccgccgcct ggtcgcgtag
     3481 acgcgcctcc agcgaggccg tgcccgtcca gatgcgatcg gcgacgcgca gcgcctcgtc
     3541 ttcgcgcgcc cgcgcccact cggccgacgg ggcctgcgcc gtcagatcgt ccatgcaatc
     3601 gaacacgcgc attcgcgccg ggagcgccgc cgcgacgccc gcccagcgcg gatcattgaa
     3661 gatgatggtc tcccacccct ggggcgattc ccggttgagg gccgccgcca tcagccggcg
     3721 gttgacctcc gccgcgatcc gcaagcggct gccccccggc acggccgcgg gcgagaggat
     3781 ccgcagactg gggggcagat cgacccccgg cgcggcgcct cgcccccgcc gatgcagata
     3841 acccggggag gcataggtca cttcccatcc gcgccgcgcc aggccgacgg ccaggtgctg
     3901 cggccgctgg aacgcgccgg accattccac gaacgagaga atcaggatgc ggccagtcaa
     3961 cggggcgggt tctttcgatg gggtcagatg cgcagggcgt cggcgagccg cgtcagccag
     4021 ggtcgccggt gcggcagctc gacctgatgg cgcggataaa agccgaattc gtagatctgg
     4081 acgatctgtt cggccgccct ggcccagctg aaacggttcg cgttcttgcg cccctcggcg
     4141 atcagatact cgcgcagtcc gtcgtcggtc agcgccagtc ccagcaggcg gaccgtttcg
     4201 tccagatcat cgggatcgac gtagagcgcg ccctgttcgc cggccacctc gggcagcgcc
     4261 atcgcatcgg ccgcgaccac cggcacgccg cacatctggg cctcaaggat cggaatgccg
     4321 aagccctcgg tgcggctcag gatcaggaac gccgccgcgt gctggaacag cacgacgcgt
     4381 tcctcgtcgc cgatgtcctg gatgatccgg acgcggccct ccaggccata ctgggcaatg
     4441 cggttcatga tcggcttgag cggatactcg tgcccctgga tgtccatcag gaagtgcagg
     4501 tcgtccatcg agtcgttgag ctggcgcaac cgggcgaagg cgcggagcat tccctcgatg
     4561 ttcttgtccg accgggccga accggaatag agcagatagc ggggggggag gttcaaccgc
     4621 gagcggatca gttcgagcgt cgcctcctcg gccgcctgcc ggaaatcttc gttcacgccg
     4681 gaatggacca ccagcgtgcg atgaaaggtc tcggggaaga gctcggccag cgcatcgcgg
     4741 gtcgcccgcg agacgcagat gatccatttg gcgcggcgca gcgccagcgg gtagagcacg
     4801 tagcgccgca gccagctctg cggcccgcgt ttgggtccga acccgatctg ctcgcggctg
     4861 aaggggagcg tgtcgtgcac ggtcagcatg accgggcagc gcgcgcggat cgagacgtag
     4921 gggaagagga cgtggagcag gtcgaacgct tcgggccgca ccatccgcga cagttgccac
     4981 atcgagcgga tcgagagcgg gtggcccttg acgcggacga tggtgaaatt ctcgcccagc
     5041 ttcagtttgc gcttgaggcg atggtgcacg aagacggtgt actggttcgc gcggtcgatg
     5101 cgcgacatgg cgaccagcag gttttcgctg tagcgcgcca cgccggaatt ggcggaaccg
     5161 aggaagcgcg catcgacggc aatctgcatc ggccagggtc cgcttcatgg ggtgggcgcg
     5221 cgccggttgc gggcggcccg cggcggcggc gcggcgctag agctggtatc cgcggcgccg
     5281 ggccagctct tccttcttgc gttgatacag cgcctgatac tcgaacgacc cttcaggagg
     5341 ggcggttttc atgccggcca gcgtgcggcg gacctcctcg tcgagcgcct cctcctgccg
     5401 caggttgtcc agaatcggtt tttcgaccca ggccgtcacc tgcgacagcc ggcgcgaggt
     5461 cttgacgtag cgcttcttga ccagctcgaa ggcgatcttg taggcgatgg cattgatccg
     5521 gtcctcggag agtctcatgc tcagagcacc atcttccttt cctgggccag cttttgtttt
     5581 gccttcagca ccagtttttc gaacgaagcg ccggtccggt cgatctcgcc gcgatggctt
     5641 tcgagcacgc ggcgggcttc ctcctcgatc tcgtcctcca tgcgtagatc gtcggtgatc
     5701 acgcggcgga ccaggccgac gatgtggtcg cggctctcgt gcagtgtcag ttcgggattt
     5761 tgcgagagcg attcaaagac gaggttggcc agatgctcga ttttttccgg tcgaagccgc
     5821 atgcgtgaac atcctttcgg tcggccgccc gccggtggcg gtttttcgcc atagtatcgc
     5881 accggcgccg catgtcaatc aatccatccc ctcgctccca aatcaaggtt cgtctgtcgg
     5941 ctcccgtcct cgtcctcggc tctcgagaag accgccggcc cacgaaaaag cccgcaaatc
     6001 ccgatgaccg gttgacttgc gggcggaaac cgatggtgcc gggaggcgga gtcgaaccac
     6061 cgacacaggg attttcagtc ccctgctcta ccaactgagc tatcccggcg cagtcggaac
     6121 ccatacacta gcgagccggt ggcggcgcgt caagcgggtt tggcgggagg aattacgcgc
     6181 cgggggatgg accgctgggc ggcccatgaa cccgcggttc acaaccaagg ggataatcga
     6241 ggcgggcctt ttggggcgat tacgaggacg gacgaagacg agagacgatg gacggatttt
     6301 tgagtgagga cgagggacaa ttttggattg agggacagcg gcgggggagg tggcgcgccc
     6361 gagaggagtc gaacccctaa cctcttgatc cgtagtcaag cgctctatcc aattgagcta
     6421 cgggcgcgca caacgagacc atatcttatc agggattggc cgcgagtcaa gtcctgaccc
     6481 acgcatgatt acgcgtcggt gatcgccgta tcgctttcga accaatcaat cggttctttc
     6541 gatttggggg atcgtgaccg tgggttggat ggccgccggg cggcgattca atccccggcc
     6601 tggatatcga accgctgacg cggttcctga tgacgcagca acctcgcgcg ccggcgggca
     6661 aggcggaacc agctcaccag ccgaacgcga ttcgctcggc caggacctgc ggcaggccgc
     6721 cttccacgat cgcctgctgc gccgcctcga tgtcgtaggg cacgcggtga taggagatcc
     6781 ggccctcatc cagatcggcc accacgtagc acgcctgcgg gttatggtcg cgcggctggc
     6841 cgacgctgcc gacgttgatc agatagcgtt cgccggtgcg gatgcggatc accggctggt
     6901 tctcgtccgg gcagctcagg tcggcgcctt gctggatcac gatggcgggc tggtgcgagt
     6961 ggccgatgaa gcagcagcgt tgcgtgaatt cattgaagcc cagccgggca tcgccgaacg
     7021 tcaggacgta accccactgc tcgggtttga gcggggaggc atggacgaag aagaagtcgg
     7081 gatcgaggca gtaggtatag gggcgttcgc gcagccagtg gcggttttcg tcggtcagtt
     7141 cgtcggcggt ccactggacc gcggcgcgcg cgatctcatt gaagaagctg acgtcggtca
     7201 tcccgatcgc ggcgtggtcg tgattgcccg ccagcgtggg gatcccgcgc tcgcgcagga
     7261 tgtcgcagca ggcgttgggg aaggcgccgt agccgacgac atcgccgcag cagaccaggt
     7321 cgcgcaggcc gagccggtcg atgtcgtcga gcaccgcccg cagcgcatga aggttgccgt
     7381 gaatatccga gaagaccgcg atgcgtcgcg gcggatcagt actcataatc tttgccggtc
     7441 agctttttca gcatgcgcgc ggcctgcttg cgaatgaccg ggcttttgtt ggtcagcgcg
     7501 cgttcccaca tcctgacctc atcctggagc ttcgcgtcgg ccggttcgtc ggcgccggcc
     7561 ggctcgcgtt cgacgtcggc atcggccggc aaggggtcca ccgtcggcgc ggcggcgacc
     7621 ggcggttccg cggcggccgg ttccggctcg tggtcgaatt cctcggccgc ttctgccagc
     7681 aggtcgggct gcgcggtcat ttcggcgacc ggatcctgtt ccgggatgat ggcggtcgcc
     7741 tcggctatct cctgagcgca gccggtcgac ggaagcggcg cgacaagcgg ttcatcggtt
     7801 tccagctctt cccattcgtc ctgctccagc gccagctcgg cgggactggg aggcgggact
     7861 tcgtaggcct cggcgacggg gggcgcgggg gactcatcgc ccgccggcgg caagtccagt
     7921 tcgtcatggg cgccgagtat ctcgtccgcc gggggcaagt ccagttcgtc atgggcgccg
     7981 agtatctcgt ccgctggggg cggatccaac tcgtcgtggg cgccgagcgt ctcgtcgatt
     8041 tcggccggcg agggagacgt ggcggcgccg ggcgcggccg acccattggc gcacccattc
     8101 aggccccgga catgctccag ttcgtccaac tcctcctcca aatccagctc ttcttcttcc
     8161 acggaagcgg agggcgtctc gggcggcgtc tcgggaggaa cgacgggctt gcgcaagacg
     8221 cgagtggcct cgttctgcat cgattcgctc agccggtcca gttcgtcctc gacactgtcg
     8281 gcgtcgtttc cgtcgtcgtc cagctccgct tcgtcgcccg gccgcatctg ctccaggtgc
     8341 tcctggagct ggaagccgag ctgcaccgtc ggcccgagct gaatcacgtc gccgtcattg
     8401 aggatggctt cggtgaccac ctcgccattg agcatcgtgt gacgccacga gcccagatcg
     8461 acaaggtagt agtccagacc gcgcttttcg atgaccgcgt ggtttttcga aacgaaccag
     8521 tcggtcaggc ggatatcgtt ctgcccgccg cggccgagcg tgatgctgtt gccgtcgagc
     8581 cggaagatct ggcctttctg gcggcctttc gtgacgacca gttcggcgac catcgattgc
     8641 tgaacgatca tcgtgccttc gacgatcgcc ggctgggcgc ggcgcaccgg ttcgcgcgtc
     8701 atccggtcgt agaagtagat cttgtgcttg ccgatcgtaa tgacgtcgcg atcgaccacc
     8761 tcgttgcgtt tgaccttgac gccgttgatg aagctgccgt tggagctgcc caggtcttcc
     8821 aggtaaaagc ggtcgttttc ccaggtgatg acggcgtggt tgcgcgaaac cgccagattt
     8881 tcaatgacga tatcgttttc cggggcgcgg ccaatcgtga tccgttcgtt cgtgaacaga
     8941 tgattctgta cgatgttgtc gcccagcttg acgataatct cgggcatggc tcgtcagcct
     9001 ttcatcgaat ctacggagac aatcgggtcg gctggcaaga tcacggaagt cgctgcgaag
     9061 tggtacaagc ctatcatgcc agacttttca tctgtcaatg ttttcggcat cgcgatgacc
     9121 ggtcttgagc caaactggta ccgatttgtc aaaaattgcc aatgcagcca tcgggcccgc
     9181 cgccgcgaca attgcatcgc caacgcgttg cgagtagaac ggttggcagc cgatccggtc
     9241 cggctgaatg gaaccgccat gaacctgacc ccgctcaatc ccgagcacct ggaggagatc
     9301 gccgattggt ggcggcgcga ccagcggctg cacccgttgg cccccgggct gctgcgcgag
     9361 cggttgttcc cgcccgaggc gcatgagccc gacctgctaa tcggcctgcg cgacgagcgg
     9421 gagcgtctcg tcggcctcgc cgccggcgag tttccggccc gctgggggga gtcgaccggc
     9481 ggggtgcggt ggctcggtct gtcgccgccg gcgctcgcgg gcggcgggct ggggccgctg
     9541 ctgggcgagc tggaacggcg gctgaccgcc cggggggcgc gcaagctcta tctgcacgcc
     9601 accccgccgt attatatccg accgggcgtg gatacgcgag acacggcgtt gatcgtcgcg
     9661 ctgctggaac tgggctggcg gcacgaggag acccatttca acctgacggt ggacctggcg
     9721 gcgtgggcgc cgcccgaggc gtccgcgatc tggggttcgc ccgccgacgc ggccgccgtc
     9781 cggcgcgcga cccccgccga tgcgaccgcg ctggcctcct tgatcgagca gcactggagc
     9841 cccggctggc ggcgcgaatc ggccctcggc ttcggacatg atcccatctc cgttttcgtc
     9901 gccgagaccg ccggcgggct ggttgggttc tcggcctacg aggtcagcca gtgcgacggc
     9961 agcttcgggc cgaccggcgt ggtggccggt cagcgcggcc aggggctcgg ccgccggctg
    10021 ctgtgggcgt gtctgtcgga tctgaagcgg ttgggccgct cccggtgcga aatcggctgg
    10081 gtcggtccgg tcccatttta ccatcgcgcg tgcggggcga cgctgggacc gacattctgg
    10141 atgatgactc gcgagctggc gcagccgggg aactgagggg ggaccatgcc gcccgagatc
    10201 accgaattca aggccctggg caagcgccgg cagcggatca tggtttatct cgacggcgcc
    10261 ccgtgggcgg agctcgatcc cgagacggtc gttcgcgagc gactgagccg gggccagaat
    10321 ctggacgagg cggcgcgagc cgcgctgctg cgcgccgacg aacagatccg ggcgcgcaag
    10381 gccgcggccg ggcatgccgc cggcggtcgc aaaacccgcc atgagctgga gcagtatctg
    10441 cgcgagcgcc ggttttcgcc gcgggccatt gcgggggcgc tccaggcgct ggccgaatcc
    10501 gaaacgatcg acgacgagca ggtcgccggg cgtcacatcc ggcgccgccg acgcgggcgg
    10561 gatctgggcc cgcggcggct ggaggccgag ctcgtggcgc gcgggattga gccgcgccag
    10621 gccgagtcgc tcgtcgacaa gaccttcagc gaagttgacg tgcgccggga gtgccgcgag
    10681 ctggcggccc gcgccgcggc gcgctaccag ccgctgaccg agccaaagca gcggcgcagg
    10741 ctggcggcct ggctgctgcg gcgcggttac ggccaggagg acgtcgaatc ggcgctggaa
    10801 tggctgcgaa ccgaggtcca ggaaaccggg cgcgaaccga ctccggactg acacattcgt
    10861 gtttttttgt ggatcagact gatacaccac gattgttcgg aagcctgact ctccgccccg
    10921 gcggcaagct tctccattat tccataagat aaataatatc agagatctgt atgcgatcgt
    10981 gtgcgcccgg ttatctcccg cggcgcacgc cgccggcatg gcatgccgct tgcattttga
    11041 gggggtcggc ggtcagggcc ggcaccggcc ccccgctcca gaaaacgaag gatgaccaac
    11101 atgagaatcc acgccatgtg tcgcaacgca cgatcaggaa agggggcgac gatcatgacg
    11161 ctgaactact gggacccgat gcgtgaattc gccaatttgc gccgggaatt ggatcgattc
    11221 ttcgagcgaa ccgacaacta ctggaccttc ccgttcacgc ggttctcgtt cctgcccggg
    11281 cgcgccgcgc gcgcctatcc gctgctgaac atcagcgagg acgcggataa cgtctacatc
    11341 gaggcgctgg cgccgggcgt cgatccgcag tcgctcaagg tggaggtgat tcgcgatcag
    11401 ctcagcatca gcggcgagaa gccggccgcg gtgcccgacg gcaaggccga gggctatcac
    11461 cgcaacgagc gggccgccgg gcgcttcgtc cgcaccttca cgctgccggc ggaggtggaa
    11521 caggacaagg tggatgcgga gtatcgcgat ggcctgttga cgatcacgct gcccaaggcc
    11581 gaggcggcca aaccgcggca gatcaccgtc aacatgaagt aagcgaccaa ttttcctgaa
    11641 tgagggcctt cccgccgggc gggaaggcct cccggaggtg aaatcccatg gctgagacca
    11701 caattgcgac caccaacacg gagctggaag cccgaccgac cgagacgcgc gaaccatcgc
    11761 ggtatcagat cccgccggtc gacatttacg agcacgacca cggcctgacg gttttcgccg
    11821 acatgccggg cgtcagctcg gaccgcgttg aggtcaatgt cgacaacgac gtgctgacga
    11881 tccgcggacg cgtccagcgc gagacgaagg gggatgccgt ctcgaccgaa ttcgccatcc
    11941 tggactactt ccgtcagttc aagctcaacc aggaggtgga tcaggagaag atcacggcga
    12001 aactgaatca cggcgtgctg acgatcgacc tgccgaaggt cgaggccgcc aggccgcgcc
    12061 agattccggt cagcgtcggc tgagagcttg cggaacgttg aaccagacgg ggcgcttccg
    12121 atgggaggcg ccccgacgct ttgagaggca agggataaag gacataaggg acatgagcga
    12181 catgagggga cgtttctcgc tcctgctctg tcgtcggcgt ccctgctgtt tcttacgtcg
    12241 cttgtctccc ttcttcacag ctcctccatg atcaatcggg gtgggcgttt gcggggttcg
    12301 gagattgtca cggccgccgt cagcatgcgg cgcgccgtct cccaatcgcg ctgcttgcgg
    12361 tagtggacgc ggaagagggg ttcgccgcgt tcaacgcggt cgcccagccg gcgctccacg
    12421 tcaatcccca cgccgtaatc gatccggtct tccttcgtgc ggcggccgcc gcccagcgcc
    12481 gccagcgcca gaccgatctg gcgggtgtcg atgcgggtga gccaacccgt cgccggcgcg
    12541 gggacaaccg cctcgcggcg cgcccagggg agccgccgcg cgtcatcgac gacgcgcggg
    12601 tcgccctgct gccattcgat caaccggcgg aaacggtcga gcgccgcgcc ggatgcgacg
    12661 gccgcgtcga gccgttcgcg ggcggccgcc gcgtcgcgcg ccagaccggc cagcgtcagc
    12721 atccgcgccg tcaacgccca ggtcacttcc ttcaggtcgg gcggtccgcc gccgcgcagc
    12781 aggtcgatcg cctcgcacac ctcgttggaa tgccccaccg cgcggccgag cggctggttc
    12841 atgtcggtca gcagggcggc gacgcggcgg ttcattttcc ggccgatgtc gatcatcttc
    12901 cgcccgagca ggcgcgcctc gcgtgtcgtc ttcatgaacg cgccgctgcc caccttgata
    12961 tcgagcacca gcacgtcggc gccttccgcc agtttcttgc tcatgatcga cgcggcgatc
    13021 agcgggatcg attcgacggt cgccgtcaca tcgcgcagcg cgtagagccg cttgtcggcc
    13081 ggcacgaagc ggtcggtctg gcccatcatc gccacgccga gccgggccag ttgccggcgc
    13141 atctgtgcgc gcgtcaggtt gaccttgaag ccggggatcg cctccagctt atccagtgtg
    13201 ccgccggtgt ggcccagccc ccgccccgag accatcggca caatcagccc gcaggcggcc
    13261 gccagcggcg ccagagccag tgagaccttg tcgccgacgc cgccggtgga gtgtttgtcg
    13321 gccttgaccc cttcgatgcc ggaccagtcg aagatctcgc ccgagcggag catggcgtcg
    13381 gtcagcgccg ccagttcgtc gccgtcgagg ccgcggaaaa agacggccat cagccaggcc
    13441 gcgagctgat aatccgggat gcggtcgcgg acatactcgc cgatgagcca gcggatcgcc
    13501 tcggcgtcga gtcgctcgcc ctggcgtttg gcgtggatca ggtcgaccgc gcgcatcggt
    13561 cgctcaatcg gtcaggatgg gattttcact gggtagagga tcgggtgcgg cgggcaccgg
    13621 catggcgccg accggttccg cgtcgggagc gacgacctcc ggctccgtgt ccgtgggggg
    13681 ctcgatcacg atcatcgccg gttcaccggc gtcgtcggcg gcgggcacgg cgggcggcgc
    13741 cgcgtcggcc gggggcgcct gctcgatcgg aatctgcgcg atcacctcat cgagccgcgg
    13801 cggcaccggc agcgcatcga cgacccagcc gccgcgcggg ttgagctccg cgaaatcggc
    13861 cggctccacg tcggcggcgc gcaccgccac cccgcgcatg aagaacagtt cgtattcgcc
    13921 ctgcttccac gccgtgaacg gcaccgcctc gtcggcgatg aagcccttga tcaccttggc
    13981 caggatgcgg acatcctgga cggcattggc gcccagcttg cgcgccttcg cctggagctg
    14041 ctcgatctgg cggcgcttga tttcgtcgtc gacgaacgcg taggcatcgc tttcgatgat
    14101 cgcgatctca tccagctcgc ggtccagttc gccgatgtag acgccgattt cggtgtcggg
    14161 gtgctcgggc gggtagcgtt cgtcgctcaa gcgtttcatg cgcgggccgg cgcagccggc
    14221 gaggatcagg gccgccgcca ggcagcagca ggcgggcagc aggcgcggga acgtcatcgg
    14281 cggaggcagc atgcggaggc acactcgcag tgggggggat cggaacggtt ccggccctgc
    14341 ggaacccgtc acatcatggg caagagcgcc gggaaggtca attgcggagg tcgccggggc
    14401 gacccttcat ccaccgccga acgattcctc ggtcaggatc gccacgacct gctcggccag
    14461 cgcggcggcg atgagcgcat tgcccttgtc gccgacgtgc aggccatcca gaaagatcgg
    14521 atcatcgcgc tgggcgaaca cgcggcgcag gtcggcgata tggaacggcg ggggcgcggc
    14581 ggtctgggcc tccgcccgca gcgcctgcag catcgcgtcg gcggcctggt cgatgtaatc
    14641 catcttgccg gggtcctcat ggcgggccag ccagcttgtt tcgccgggcg tcaggggctt
    14701 gtattgcgcg gccagatagg gctggatggc gacgaggaac gggacgccgg cgcggcgcag
    14761 ggcgtcgccc gccagagaca tgttccaggc gaagcggcgc gcaaccaggc cggggtcaac
    14821 cgggtcgccc cacatcgcgg gcggggcatg ggcaaagggc tcggccccgg cgatgcgcat
    14881 cccgccgcgg atcaattcga aatacatgat ctcctcgttg gcgcggaaac tgaggatgtc
    14941 catgccctcg aagccccagt gggcgtcgtt gacgcccgtc aggctgatga ccaggtccgg
    15001 ttcccagcgg ctgatgtcgt tctcgatgcg gatccgttcg tgcgtgctgg accaggccgg
    15061 cacggcggca ttgaacactt cgacgcgcca gccggcgagc gcggggcgtt gggcgagcgc
    15121 ggtttccagg cagccggcga tcgtcgattc gagcgcgggc gcgccgacgc tgtaggccag
    15181 cgagccgccg gtcaagaaga cgcgaaagac gccgggcggc ttgggcagga cgagcggtcg
    15241 ccggctgcgc aactggtgcg cgttgatcgc cccttcggtc tggtcgccgg gctcgggggc
    15301 ataaccgatg aacggcatcg gccggcagcg caagttccag gaaatgcgct ccagatcgac
    15361 cgccgacggc tcgcggtagc attggcggac gtggtcggcc cagtcggcgt aatcctccgg
    15421 cgcgatcgcc gcgcgaccga cgccggccag cgtctgatcg gccaggcggg tctggattac
    15481 gtagcgcaag tatcgccgtt cgcgcagcag cggcagcgcc cagatggcca gcgcgacggc
    15541 gacgaccagc accggcagga acacggccca ggtgatgaag aggaggcggc ggtcgcgcga
    15601 gagcggcggg cgggaagtca tggcgcggat tataggggtc ccgccgcggg aaatccaatc
    15661 cggcgaccaa cgcggcgcag gccagcgccg gaaacgagat gccgcgcgga tgcggcggat
    15721 gggaggcaag ttcttcgccc gacggcgcgc cgcgcgggac tttctttcgc ttgcaccgaa
    15781 tcgccgcgcg gttagaatcg gtcgttcggc cctggccgcg gacgcacgca gggccccgcc
    15841 gtcgaagccc agcaggattg tcagtcgcat ggcgaagaaa aaagcctttg aagacgcgct
    15901 ccccgcgcgc agcgagaact tctccgaatg gtatgtcgac gtggtgcgca aggccgaact
    15961 ggccgactac tccgacatcc gcggctgcat gatcatccgg ccctacggct acacgctgtg
    16021 ggagaacgtg cgcgacgcgc tggaccggcg aatcaaacag accggccacg tcaacgccta
    16081 ctttccgctg ttcatccccg agagctacct gatgaaggaa gcggagcacg tggaagggtt
    16141 cgcgcccgag gtgccgtggg tgacgcatgt cggcgaggag aagctggagg aacggctcgc
    16201 gatccggccg acctccgaga cgatcatctg cgcgacctac gccaagtgga tccagagcta
    16261 ccgcgatctg ccgctgctga tcaaccagtg ggccaacgtc gtgcgctggg agaaacggac
    16321 gcggctgttc ctgcgcacga ccgagttcct ctggcaggag gggcacacct gccaccgcac
    16381 cgaggaggag gcggtcgtgg agaccctcaa gatgttgaag gtctaccatg atttcctgcg
    16441 cgaggatctg gcgctgccga cgatcatggg caagaagacc gaggcggaga aattcgccgg
    16501 cgcggtcgat acttactgcg tcgaggcgct gatgggcgac ggctgggcgc tgcaggccgg
    16561 gacgtcgcac atgctggggc agaacttcgc caaggcgttc aatatcaagt tcctcgatga
    16621 ggacaacacc gaaaaattcg tctggcagac gagctggggc gtctcgacgc ggctggtcgg
    16681 cgggctgatt atgggccagg gcgacgacgc cgggctgcgc ctgccgccgc gggtcgcgcc
    16741 gatccaggtc gtcgtcgtcc cgatcatctt cgaggactcc aaggagcagg tgctggccgc
    16801 cgcgcgcgag ctgacggcgc ggctcgagac ggcggggttc cgtgtccggc tcgacgaccg
    16861 cgaatggcac aacgccgggt tcaagcgtca cgactgggaa ctgcgcgggg tgccgctgcg
    16921 cgtcgaggtc ggcccgaagg atctggcgca gaaccaggcg tttgccgcgc ggcgcgacac
    16981 cggcgagaag gaggccatcg ggctggacgg cctgccggcg cgcgtcggcg aactgctcga
    17041 cgcgattcag cggacgatgc tgcgccaggc cgaggagttc ctggccgggc acacgaccga
    17101 agtagggagt tacgaggaat tcaaacgggt gctcgatgag ggcaaggggc tgatccgcgc
    17161 gccgtgggac ggcgatccgg cgacggaggc gcgcgtcaag gaggagacca aggcgacgat
    17221 ccgctgcatc ccgctggagg ggggcgaggc gccggccggg gccacggacc tggtgtcggg
    17281 ccgtccggcc cgtcatctcg tgctctatgc gcgggcttat tgagcgccgg gcggtctgta
    17341 aacattacaa gcgaagcgcc gccctcctca gaattcgtcg tccgtcgtct cgcggcttcg
    17401 tcctcgctcg aaaatctgtc cccgattctc gggttgcttg gatcggttct gccgtccctg
    17461 cgggactctg ttgccatggg tatcccggtt cccgccactg acgtggcggg ctgaagtctg
    17521 acgtccctga cgggattgcc cgggtgatcg gtccgtcaag gccagggtcg ttcggatcgg
    17581 ccgtttacct ccatttccat cccgcgttgt gaaccgcagg ttcaggagca actcgtttat
    17641 cggccccagc ggcgccccgg agcttctctt tcgtaagcca tggacaagag gccgaaggcc
    17701 gaatcctggg cgtggagagt tcgcggggcc gcgcgaagag gatgcgcgtg ggcccgcagg
    17761 cggccgtccc cgcagtcgcg gcccggtcac cgcaaaacgt tccaggtatc gtcgtttcaa
    17821 catctgacac atttggaacg gtgcgtgcta cggggcgaat gtgcgggttg aatcggccgt
    17881 cgactggagc tccctcccct cgaacgccgg aagcatccac cgccagctta acgggcgttc
    17941 gtctgggtct cccctttcgc ccccgggcca ggagcagtct cataatgaag aatcggaagg
    18001 tactcaatca tcaggtgacg gcgggcggtc agccctctgg cgacgacctg gtttatctga
    18061 aacagcacca gtttcgttcg gtcgtgaacc tgcggacgga aggcgaagcc gaccagacgc
    18121 tttcgcccga cgaggaacgc cggctggtcg aggggctggg gatggcttat ctccatgttc
    18181 cggtttcggc ccaaaccatc tcgccggaac tgctggggca gtttgtcgcg gagttcgacg
    18241 acctgcccaa accagcgtat gttcactgcg agtcggggcc gcgggccgga ttgttcgcaa
    18301 tgctctggct gggcgtgaac caggggctga cgggcgaaca ggcgatcgcg cgcgggcgcg
    18361 aatacggctt cagcctggat aacccgcaat tggtcgaacc ggtccggcga tttctggaca
    18421 gccaggaacg cgtgcagtaa cgccggccgg cggtcagcat aaaaaaaaac ggcgggagca
    18481 gagaagcctc ccgccgtgtt gttttttgat acggtttcag cgcgccgcgg ccgcagtgcc
    18541 cgccagctcc gcgaagactt ccttgtgctg cgtctcttcc caggggaaac cttcctcggt
    18601 ccggccgaaa tggccgtagg cggccgtccg gcggtagatc gggcgcttca ggtcgagact
    18661 gcgcaggatg ccggcggggg tcaggttgaa cgtttcgcgg acggccttga tcagcaggtc
    18721 ctcgtcgaca cggccggtgc cgaaggtatt gacgttgatc gagaggggct gagcgacgcc
    18781 gatggcgtag ctcagctgga cctcgcagcg ctcggccgcg ccgccggcga cgatgttctt
    18841 tgcgatccag cgcgcggcgt aggcggccga gcggtcgacc ttggaggggt ccttgccgct
    18901 gaacgcgccg ccgccgtggc ggcccatccc gccgtaggtg tcgacgatga tcttgcggcc
    18961 ggtcaggccg gtgtcgccgt gcggcccgcc caccacgaac cggccggtgg gattgacatg
    19021 aatgatcagg ttcttgtcga ccagttcggc cggcagcacg gggtcgatga tgtgctcaat
    19081 gacctgccgg cggatctcgg cctgttcctt tttcaggtcg tcgcgggtgg gcgagttgcc
    19141 gccgtagagc ggcgcgtgct gcgtggagag gacgaccgtg tggacgcgca gcggcttcca
    19201 ctcctcgtcg tattcgatcg tcacctggct cttggcgtcg gggcgcagcc aggcgatggc
    19261 gccgtcgcgg cggacggcgg cctggcgctt gaccagcttg tgcgccagtt cgatcggcag
    19321 cggcatcagc tcgggggttt cattgcaggc gtaaccgaac atcatcccct ggtcgccggc
    19381 gccctgctcg tggtcggagg aggcatcgac gcccatcgcg atatcgggcg actgggtgtg
    19441 gatcgactgc aggtacttgg ccccctcggc gcagaagccc atcgccggat cggtgtagcc
    19501 gatctcttcg atcgtttggc gggcgacgcg ctggtaatcg atgcgggcct gcgtcgtgat
    19561 ctcggcggag acgacgacca gatcggtcgt gacgagcgtt tcgcacgcca cgcgcgactg
    19621 cggatcctcg gtcagcaacg cgtcgaggat ggcgtcggag atgttgtcgc aaaccttgtc
    19681 gggatggcct tcggaaaccg attcggaggt aaacaggcgc gtgcgcggcg tcatcattca
    19741 caatcctctc gcccgggtca cggcgggcgg ctcaatatgg gggatcgaag ttacggatct
    19801 gcccctggca tggcggcggg ggcgccgcgg aggccggttg tcactgtagc gggaaggggc
    19861 gttgaaatca cgcaaaaaag ggccggcgac gaccgaccgg gtgcggtgga aacccgtgaa
    19921 accaggcggg tcttacgcgc tatcaatatc ggtatcggta acgggatggt gaatcgggat
    19981 cgaataagcg gacttgcccg accgcgccga tgtgtgtata tacgaatagg gcaccgttca
    20041 ctcgcgaccg acccgcggcc ggtgcctcgg ccgcgagtca ggctg
//
LOCUS       DAOPCO010000041        19944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2831_length_19944_cov_5.955754, whole genome shotgun sequence.
ACCESSION   DAOPCO010000041 DAOPCO010000000
VERSION     DAOPCO010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 19944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19944
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2831_length_19944_cov_5.955754"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLS90_05065"
     CDS             complement(<1..1243)
                     /locus_tag="PLS90_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01806.1"
                     /translation="MNAASTVAPPADARTPPPQPAIELRGVHKFFSRVEGRSHTLKET
                     LLASLAQRARRDLIHAVNGIDLTVARGEAVGLIGPNGSGKSTLLKLIAGISQPTRGTV
                     RSDGRLLGLIELGAGFHPDLTGEENVRLQGSIYGLDAAEIEARMDAIFDFAEMHDFRS
                     MPVKHYSSGMFLRLGFSIAVHADPEILLVDEVLAVGDLEFQERCLARIREMRRAGLTL
                     LLVTHYPEQVERLCDRVIWLERGAVRALGPTFEILTAYYREMLDVRYGVSQGPFSMQT
                     VTVGLPGRFGSGEARIESFRIMDAAGRSRLTFRRGERLIVELEYSAQPGVEAVDCMMP
                     LSAQDGTLVTLWRLIREHAPSRPVDGRGRIRFEAPTLPILPGRYELTVSLTRLDDPYQ
                     HYDMLYKIFYLTIEKELDWATS"
     gene            1647..3923
                     /locus_tag="PLS90_05070"
     CDS             1647..3923
                     /locus_tag="PLS90_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPK01807.1"
                     /translation="MSDLAELLSHLNPQQQAAVTTTDGPLLVIAGAGSGKTRVITHRI
                     AWLIERQGVAPWQIFAATFTNKAAEEMRHRVGRLLPGADVARLAIATFHSLCVTILRR
                     EAQAVGLSDRFTICDDTDQMALIKDCLGSMGIGAAQIKPEQVRNYIGAAKILMLSPDQ
                     ARTRLDSEFGPVCSQVYARYQERLLAHDAVDFDDLLLHVVHIFQTHPDVLRHYQERWS
                     YVLVDEYQDTNAVQFELVRLLAEGHGNICVVGDEDQSIYSWRGAQIENLLEFPSRFPG
                     TQIIRLEQNYRSTEAILKAASAIIANNTQRLGKELWSDRGEGEPITLIEARSELEEAA
                     HVVEAIHWLHKVHGIDLRDIAIFYRVNALSRVFEDQLRQRGVPYRVIGGIKFYDRAEV
                     KDLLAYLRLCVNPRDGIALSRIINKPARGIGGKSLAQIFAEAVRQQVSIWDVMLRARQ
                     KDCPLEISTKARAGVLDLIDRVEAWQADVADRKPHEMLERILRETNYEESLGKANDLD
                     AISRRENISELLNALEDFEANSPGATLADFLERVALINAQDDMSDEPAVSLMTLHCAK
                     GLEFRVVFLVGMEDPIFPSKRAVVDQGHFEEERRLFYVGITRARDLLFLSRADERRLY
                     GRPAYNPPSLFLSEVPRELVRSLAEARRSWSEQRSRLATASHAPAGAGRRERVRQPAL
                     HEPAAEAAPVVDEDAPFPVGSRVSHSRLGAGEVVAIKGSGAKRKLSVRFDAGLELEVV
                     EQFGGLRRLDDETPDLPF"
     gene            complement(3935..4336)
                     /locus_tag="PLS90_05075"
     CDS             complement(3935..4336)
                     /locus_tag="PLS90_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HPK01808.1"
                     /translation="MPYRLLADAMLLLHLAIVAFNVLGLILIWVGRWRGWGWIRHPGF
                     RWPHLLVVAFVALLPCLGQICPLTVWEWELRRLAGSAAPAPPGLIERLVQAVLYYDLP
                     PSAFLVGYGIFFLLVLGTWFRIPPKRPPGPT"
     gene            complement(4410..5555)
                     /locus_tag="PLS90_05080"
     CDS             complement(4410..5555)
                     /locus_tag="PLS90_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01809.1"
                     /translation="MKPDRPLRVLRVITWLPVGGIERRLVATLPRFDRERFELSLVCI
                     RERGALADELEAAGIRVDCIPFRKRWDPRALRQLTDLMRERRIDIVHSHMYRSNVPAT
                     AAARRARTPVVWAQVHNVGTWETRRQVWIDRWLCRWRTGVIAVSEKVRRDVMATLNLP
                     PERVPVVYNGVDLARFAAARPRRPAVRAREQVGEHDVVFLFAARLVEQKRATDLLTAF
                     ADLQQQPGGERLRAWIVGDGPLREQLERQAEALPQPAAVRFFGRRTDVEDFYAAADIF
                     VLPSTREGFSNALVEAMATGAAVIATDVGGNAEAVRDGQDGLIVPPLRVDALRDAMAR
                     LAGDAALRRRLADAAAARARRFSLEAMVTQLEELYAESYRRAVAPHA"
     gene            5753..7033
                     /locus_tag="PLS90_05085"
     CDS             5753..7033
                     /locus_tag="PLS90_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPK01810.1"
                     /translation="MLRRELGLMDAVMMGLGSILGTGIFVSIGLAAGIAGPAVIVALA
                     LAAVLAAFNALSSAQLAAAHPVSGGTYEYGYEFLRPSLGFTAGWMFVLAKSASAATAA
                     LGFAGYLTASTGLFAGGRPVVACVTVALLTGVVLSGMRRSSRLNIAIVAITLLSLIAF
                     TLAGLGVQAARGGTEFYTPFFAGDGAGEVAANLLHACALMFVAYTGYGRIATLAEEVA
                     DPQRTIPRAIIATLLVAGVIYVGVAAAGVGSVGSAFLQRAAAGEAAPLALAARRFGIP
                     LLPALVAVGAATAMLGVLLNLLLGLSRVLLAMGRRGDAPRALARLDRSGATPVAAVIA
                     VGGLIAGLTLIGSVRTTWSFSAFTVLIYYAITNAAALALPPPARLFPRWMAAAGLAGC
                     LFLAFWVEQRIWLSGLLLIAAGLGWHRLARRLPR"
     gene            7149..7355
                     /locus_tag="PLS90_05090"
     CDS             7149..7355
                     /locus_tag="PLS90_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01811.1"
                     /translation="MVDLHPEYVVDAQRRPKAVLLPLAEWERILEALEDLEDLRAYDE
                     ARNQAQDALPFDQAVREIRQGRVE"
     gene            7397..7624
                     /locus_tag="PLS90_05095"
     CDS             7397..7624
                     /locus_tag="PLS90_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPK01812.1"
                     /translation="MARIDRENQKRIIEAIYQLAECPRPSGCKKLIGRDAWRVRVGTY
                     RIIYEIRDDRLLILVIAIGDRKDIYRQSFGS"
     gene            8380..8907
                     /locus_tag="PLS90_05100"
     CDS             8380..8907
                     /locus_tag="PLS90_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015503910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPK01813.1"
                     /translation="MPRPSWDEYFMMMAEVAAMRGNCVKRRVGAVIVLERQILSTGYN
                     GTPKGITNCCDGGCPRGSSVADSGGSLEACLGVHAEENALIQAAAHGVRIRGATLYCT
                     LCPCSYCAKSIINAGIAEVVYRDVYAMDDVTARLFQEAGVKLRRMDPAEVSVHPRYAR
                     RERGRAPLRQGLLEI"
     gene            9238..10725
                     /locus_tag="PLS90_05105"
     CDS             9238..10725
                     /locus_tag="PLS90_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01814.1"
                     /translation="MTPPEAPPLPSTLPATLMAYLRQQPRAVRMFNRRLEVLWDNCPD
                     RAAEFPLFTGPTGAGVTVPPPGPERNAWPVQRVLEHGQPAERFYVPAGPDAPAGTCFR
                     LRAWPLESDAGEFELVVEEIDRVNSRICFDERLKTLDEELAELIDHVAAVLSGEEGAH
                     ALRLRLTNPNLQPCHEIKQCERETCPAYANEENPRCWDVDNTLCHKGMEVQNPLGKFR
                     YCDQCKVYLLACPDPLTRVGENFNRLLSLLNLKHQEALEAQQHVQQADKLATVGELML
                     GLAHEVKTPLSVIIGRLDCLALELESLTLREIGEDLEVMRQHAQRMSTVLKDVLTLAR
                     PAPPSPRALDLATSVREALAMATTTLERARIRVENEVPTGLPTVFADPLQIQQVLLNL
                     IINARDAMPDGGVLRLETRVAEGEADVLELHVRDSGHGIAPEDQQRVFSPFYTTKAER
                     GGTGLGLAVCQRIMMHHGGRIRVESRPGAGTTIVLSFCIHEGAVP"
     gene            10722..12155
                     /locus_tag="PLS90_05110"
     CDS             10722..12155
                     /locus_tag="PLS90_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK01815.1"
                     /translation="MSGATLGSPSRILVIDDEVDMLTNYRRMLERAGHDCRLESDPRR
                     LEPLLSEYQPDLVLTDLVMPHGSGMEVLDRVRQFDPTLPVILLTAHGSIESAVEAMKR
                     HATDYLTKPFSIEELLGKIQTALSRRLIEQSDEPGASGPPPAGNEGWRREIIGISPAI
                     EKVITLARKVSRTDVNVLIQGESGTGKEVFARLIHRLSPRSQEILVPADCASLPENLL
                     ESELFGYRKGAFTGAHSDKMGLFEFAHKGTLLLDEIGEMPLALQAKLLRVLQERRFRP
                     IGGREQIEVDVRVIAATNRDLAQALAEKTFRSDLYYRLNVVTLRLPPLRERLEDVPLL
                     ARHFLSQFARDNRLAVDAITPRALELLKAYDWPGNVRQLQNVVEHAATMAGEGAIGPE
                     HLPEEFEPLRAAERAAREGDEASLFSRKTELVDHFERDYLVSLLIDQRFNISRAATVA
                     GCHRRTLYRMIHRHRINLAELRRQHPA"
     gene            12451..14430
                     /locus_tag="PLS90_05115"
     CDS             12451..14430
                     /locus_tag="PLS90_05115"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01816.1"
                     /translation="MKPAGPLAASHFKFVGICLVAALTAAGLPVGAQHAAGPPVIDVV
                     GVTPRQVAIAPDDVYDEVASGLDVVGVGNKVYLEAHTAEDGLTFSWSIVSRPADSTAQ
                     LSATSGKLVTFRPDKVGPYVVRLTSTDGGGQALQPDERRILAANWAGAGLFDTHNPPA
                     PRAPECGAGFCHGGNNAKPELNVAPEWIKSNHAQALQLRLKGERGAHYDVSCLPCHTV
                     GWNQNAAAVNHGFDDVAFEIGYDVAQIPALVHAAAEQGLDNFPELPAALQDMSSIQCE
                     SCHGAGSQHPSRLGEEGKGIAGADLGYEQCAQCHDSATGFQQGFYQWSQSQHPITQEA
                     GEGHVQDNATCLPCHTGEGFVQKMVNNETPALGQAFHGITCAACHDPHYSENPHQLRL
                     TGDMTFPSGQQTSAVAGSMVGMGGLCMRCHNSRVSNLAGTVLGSSRGAHYGTQGDMLM
                     GINGWDFGLPFNASSAHIRVVEDTCVACHMAEPTESGPGVITPPAVGAHSFAMRDTMG
                     TADPADDTTNAVNACSGCHEALDTYDRRARGDYDGDGEREGIQSEVQGLLALLRPGLL
                     EFPGVSEEGPFEKINIDAAGFNLLTNDQRAALYNYNFVVLDGSLGVHNTAYAVQLLQR
                     AYYGVYGRPISNDYPAMALRGPVQQNSASGWADYR"
     gene            14523..14897
                     /locus_tag="PLS90_05120"
     CDS             14523..14897
                     /locus_tag="PLS90_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01817.1"
                     /translation="MVWIITPHRTVHQRIERLLRPLPAEVVRRERVLEDPRGSARPKP
                     RPQLVVLDVDEDMEQGARVIEALRRGRLDAPLIVLTRAFTRDFGARILSLGVRYYFSQ
                     DFCESEFREVVQSLLRLPASEV"
     gene            14960..15640
                     /locus_tag="PLS90_05125"
     CDS             14960..15640
                     /locus_tag="PLS90_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01818.1"
                     /translation="MKPLRKGQRRRPAGRPRRMITGLLLAAAIAVVLLSVQPAPATIR
                     GSAHDFSGVDPEQQICIFCHTTHNADTTVTDAPLWNHDVTTKNYQVYNSPSLDATPGQ
                     PAGASRLCLSCHDGTIAVDSYGDRTGVIFLGGDAAIGADELTNDHPVSFTYDDSLAVQ
                     DGELFLPSSAPSGMGGTIAQDLLFNNQLECASCHDVHNGPAAAAVNDHLLTVTQVQSQ
                     LCLTCHNK"
     gene            15965..17068
                     /locus_tag="PLS90_05130"
     CDS             15965..17068
                     /locus_tag="PLS90_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01819.1"
                     /translation="MIPRLSTLRLRPCLLPALALLLSLAGCARAPRVEPAPGPLFFPP
                     PPLPARVQFLGSVSSPLDLPRPRSGFADFILGPPPLKYPLVKPLAAALRDGKLYVCDS
                     VLNTVIIYNLLTGEAGLLPGDAGNGKLQQANNLEFDEQGNLYVADKKRQAVLVYDARG
                     AFVRAVGRPGEAEPVDMAPAGGLLYVCDIKDHQIEVWDAASGALVRQFGSKGNGKGEF
                     YLPSQVALGPDGNLYVTDTGNFRVQKLTPEGEFLATVGGHGTALGNFAWPKGLGLDGD
                     GRLYVADSRFANVQIFDPEGRLLLFFGGPGPTRGHLDLPAGLSVMPWPEAPWLRERLA
                     AGFVPEWLVIVVSQKGDGMVNFFAVASDDAPAP"
     gene            17065..17631
                     /locus_tag="PLS90_05135"
     CDS             17065..17631
                     /locus_tag="PLS90_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK01820.1"
                     /translation="MNRALPRLASSRLALAGLALLLASCAPPRRQEVLSFFFDGVPGA
                     GEAGAPAGAADGLSPLFHPEPAEALSAPAAPPVGSVHAPVFQRQCNTCHDPTSGLAPR
                     GKDAALCDRCHQEQREREGWNHGPINLGTCIPCHRAHESPYPHLLDKPLPELCLDCHK
                     EDLSRPSEYHSETEVEACTVCHDPHRMY"
     gene            17730..19835
                     /locus_tag="PLS90_05140"
     CDS             17730..19835
                     /locus_tag="PLS90_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01821.1"
                     /translation="MTLRSAPIRRAVALCLGLIGLAGGGERAGAGMADSQCLKCHGEA
                     WIADARPEALAAMVRHPDGQPVLRAEEAIDELYTPAAIFAGSVHAGLSCTDCHQTGDR
                     LPHDQRLPEIACGDCHSEVEDALRASAHGGAAQQGRARPTCTDCHGPAHEIQAFGEPR
                     THAAAVRETLACMECHRDGDDAEAHPAATYRENVHGRALFEKGLSISATCVDCHGAHG
                     VLPAADPASLMHPRNVPQTCSRCHEGIAEVYYGSVHGRHLLEGDETAATCTSCHGSHG
                     VEAVADHFLLGVINECSHCHIDLGRSYLTSYHGKAAHLGYAEAAVCSSCHGAHDILPP
                     EDPASRVAEANLVATCGACHEGVNANFIQFDPHVDLRDRAANPEVFWTWLVMTTLLLG
                     TLAVFIPHGLLWFQRTLVERLRHPRGFHGVMHEERWVMRFNPVHRITHALIIISFMGL
                     VLTGFPLKYSHTEWALGITRLFGGIPMMGLLHRAFALITFAYVGVHVAFLVIFFWKHC
                     PRPRRRYLFGPDSMVFSLRDLKDFIAMVRWFLWLGPRPKFDRWAYFEKFDYWGEIWGV
                     IVIGGTGLMLWLPEFFTRWLSGWVLNCAMVVHSIEALLAASVIFLVHFFNTHVRPEKF
                     PIDMVMLTGRMTESEMKEERGDEYARLVQSGELERRLVPPVALKWRLLGAVAGLMAFA
                     LGVTLIVLAITTEFDQIFG"
BASE COUNT         3175 a         6288 c         7325 g         3156 t
ORIGIN      
        1 cgctggtggc ccagtccagt tccttctcaa tggtcagata aaaaatcttg tagagcatgt
       61 cgtaatgctg atacgggtcg tccaggcgcg tcagcgagac ggtcagctcg tagcggccgg
      121 gcaggatcgg cagcgtcggc gcctcgaacc ggatgcggcc gcggccatcg accgggcgcg
      181 acggcgcgtg ctcgcggatc agccgccaca gcgtcacgag cgtgccgtcc tgcgcgctga
      241 gcggcatcat gcaatcgacc gcctcgacgc ccggctgggc ggagtattcg agctcgacga
      301 tcaggcgctc gccgcggcgg aacgtcaggc gcgagcggcc tgccgcgtcc atgatccgga
      361 acgactcgat gcgcgcctcg ccgctgccga atcgcccggg cagccccacc gtgaccgtct
      421 gcatcgaaaa cggcccctgg ctgacgccgt agcgcacgtc gagcatttca cggtagtagg
      481 ccgtcaggat ctcgaacgtc gggccgaggg cgcgcaccgc cccccgctcc agccagatga
      541 cccggtcgca gaggcgttcg acctgctcgg ggtagtgcgt gacgagcagc agcgtcaggc
      601 cggcgcgccg catctcgcgg atgcgcgcga ggcaccgctc ctgaaactcc aggtcgccga
      661 cggccagcac ttcatcaacg agcaggattt ccggatcggc atggacggcg atggagaagc
      721 ccaggcgcag gaacatcccc gaactgtaat gtttgaccgg catcgagcgg aaatcgtgca
      781 tctccgcgaa atcgaagatc gcatccatcc gcgcctcgat ctccgcggca tccaggccat
      841 agatcgaccc ctgcaggcgg acgttctcct cgccggtcag gtcgggatgg aacccggcgc
      901 ccagctcaat caggccgagc aatcggccgt cgctgcgcac cgtcccgcgc gtcggttgac
      961 tgatgccggc gatcagcttg agcagcgtcg attttcccga cccgttggga ccgatgaggc
     1021 cgaccgcctc gccgcgcgcg acggtcaggt cgatgccgtt gaccgcatgg atcagatcgc
     1081 gccgcgcgcg ctgggccagc gacgcgagga gcgtttcctt gagcgtgtgc gagcggccct
     1141 cgacgcgcga gaagaacttg tgcacgccgc gcagttcgat ggccggttgc ggagggggcg
     1201 tccgggcgtc ggcgggcggg gctacggtgg aggcggcatt catggcgtca agttagccga
     1261 tcggcgggtg aaaaggaaga cggccctctc cggcccgccg aaatgaacag gttcctccct
     1321 ggaaccgtcc ctcgtccctc cctcctgatc gctcccggag gccttaccaa aatttggctc
     1381 tcggctctcg ccccgcgccc tcggctcccg aaaggcggcg tgtatccctt cgtattgaac
     1441 cgtcggttcg tgggcaactc ggctccgtga tcgaattcgg gcggttccgc cgtccctgcg
     1501 aaactctttc aattttttct cccgttttac tgtagaggat gggggcggga ttgacggcct
     1561 ggggttgcgg cgggcagaat ggggaagcga ccgttgacgc ccgtgccgcc ccgcccgacg
     1621 cggccatccc cgctggaccg tccgccatgt ccgacctcgc cgaattgctc agccacctca
     1681 acccgcagca gcaggccgcc gtgacgacca ccgacggccc gctcttggtg atcgccggcg
     1741 ccggttcggg caagacgcgc gtcatcacgc atcgcatcgc gtggctgatc gagcggcagg
     1801 gggtggcccc gtggcagatc ttcgccgcga cgttcaccaa caaggcggcc gaggagatgc
     1861 gccaccgcgt cggccggctg ctgcccggcg ccgatgtcgc ccgcctcgcg atcgccacgt
     1921 ttcactcgct ctgcgtgacg atcctgcggc gcgaggcgca ggccgtcggg ctgtcggacc
     1981 gcttcacgat ctgcgacgac accgaccaga tggcgctgat caaggattgc ctggggtcaa
     2041 tggggatcgg cgccgcgcag atcaagccgg agcaggtccg caattacatt ggcgcggcga
     2101 agatcctgat gctgtcgccc gaccaggcgc ggacgcggct cgacagcgaa ttcggcccgg
     2161 tctgttcgca ggtctacgcc cgctatcagg agcggctgct ggcccacgac gcggtcgact
     2221 tcgacgacct gctgctgcac gtcgtccaca tctttcagac gcatcccgac gtgctgcggc
     2281 attaccagga gcgctggagc tacgtcctcg tcgacgagta ccaggacacc aacgccgttc
     2341 aattcgagct ggtgcggctg ctcgccgaag gccacggcaa catctgcgtg gtcggcgacg
     2401 aagaccagag catctattcc tggcgcggcg cgcagattga gaatctgctg gagtttccca
     2461 gccgctttcc gggcacgcaa atcatccggc tggagcagaa ttaccgctcc acggaggcga
     2521 ttctgaaggc ggccagcgcg atcatcgcga acaacacgca gcgcctgggc aaggaactgt
     2581 ggagcgaccg gggcgagggg gagccgatca cgctgatcga ggcgcgcagc gagctggagg
     2641 aggccgcgca cgtcgtcgag gcgatccact ggctgcacaa ggttcacggg atcgatctgc
     2701 gcgacatcgc gatattctat cgggtcaatg cgctgtcgcg cgtgttcgag gatcagctcc
     2761 gccagcgggg ggtgccctac cgcgtgatcg gcggcatcaa gttttacgac cgcgcggaag
     2821 tgaaggatct gctggcctac ctgcggctgt gcgtcaaccc gcgcgacggg atcgcattgt
     2881 cgcggatcat caacaagccg gcgcgcggga tcggcggcaa gtcgctggcg cagatctttg
     2941 cggaggcggt gcgccagcag gtctcgatct gggacgtgat gctgcgggcg cgccagaagg
     3001 attgtcccct ggaaatttca acaaaggcgc gcgccggcgt gctcgacctg atcgaccggg
     3061 tggaagcctg gcaggccgat gtcgcggacc gcaagccgca cgaaatgctg gagcggattc
     3121 tgcgcgagac gaactacgag gaaagcctgg gcaaggcgaa cgatctggac gcgatctcgc
     3181 ggcgcgagaa catcagcgaa ctgcttaacg cgctggagga cttcgaggcg aacagccccg
     3241 gcgcaacgct tgccgatttc ctcgagcgcg tggcgctgat caatgcccag gacgacatgt
     3301 cggacgagcc ggccgtttcg ctgatgacgc tgcactgcgc caaggggttg gagttccgcg
     3361 tcgtgttcct ggtcgggatg gaggatccga tcttcccgag caagcgcgcc gtcgtcgacc
     3421 aggggcattt cgaggaggag cggcggctgt tctatgtcgg gatcacgcgc gcccgggatc
     3481 tgctcttcct gagccgggcc gacgagcggc ggctctacgg ccgtcccgcc tacaatcctc
     3541 cgtcgctctt cctgagcgaa gttccgcgcg agctggtgcg ctcgctggcc gaggcgcggc
     3601 ggagctggag cgagcagcgc agccggctgg cgacggcgtc gcacgccccg gcgggcgccg
     3661 gccggcgcga gcgggtgcgg caaccggccc tgcatgaacc ggccgccgag gcggcgccgg
     3721 tagtcgatga ggatgcgccg tttcccgtcg gcagccgcgt cagccacagc cggctggggg
     3781 ccggcgaggt ggtcgcgatc aaggggagcg gcgcgaagcg caaactgtcg gtgcgcttcg
     3841 acgccgggtt ggaactggaa gtggtcgaac agttcggcgg gttgcgccgg ctggacgacg
     3901 agactcccga tctgccgttc tgacgggccc gagatcacgt cgggccgggc gggcgcttcg
     3961 ggggaatccg gaaccacgtc cccaggacga gcaggaagaa gattccgtag ccgacgagaa
     4021 acgccgacgg cggcagatcg taatacagga cggcctgcac gaggcgctcg atcaggcccg
     4081 gtggcgcggg ggccgcactg ccggccaggc ggcgcagctc ccattcccag accgtcaacg
     4141 ggcagatctg gcccaggcag gggagcagcg ccacgaacgc cacgaccagc agatgcggcc
     4201 agcggaatcc ggggtggcga atccagcccc agccgcgcca gcggccgacc cagatcagga
     4261 tcagaccgag cacgttgaac gcaacgatcg ccagatgcag gaggagcatc gcgtcggcaa
     4321 gcaggcgata cggcatgatc gcggggggcg gggccggcgg gacgcgcccg cgggtccggc
     4381 cgtgctccag gattcatctg gccggcgcgt caggcgtggg gcgcgacggc ccgccgatac
     4441 gactccgcat acagctcttc gagttgggtc accatcgcct ccaggctgaa gcggcgggcg
     4501 cgcgcggcgg ccgcatcggc cagccggcgg cggagcgccg cgtcgcccgc gaggcgtgcc
     4561 atcgcgtcgc gcagggcgtc gacccgcaag ggggggacga tcaggccgtc ctggccgtcg
     4621 cggacggcct cggcgttgcc gccgacatcg gtggcgatga ccgccgcgcc ggtcgccatc
     4681 gcctccacca gggcgttgga gaatccctcg cgggtcgagg gcagaacgaa gatgtccgcg
     4741 gcggcgtaaa agtcctccac gtcggtccgc cggccgaaga accgcaccgc cgccggttgg
     4801 ggcagggctt cggcctgccg ttccagttgt tcgcgcagcg ggccgtcgcc gacgatccac
     4861 gcgcgcaggc gctcgccccc cggctgttgc tgcaagtcgg cgaaggccgt cagcaggtcg
     4921 gtcgcgcgct tctgttcgac cagcctggcg gcgaagagga agaccacgtc gtgctcgccg
     4981 acctgctcgc gggcgcgcac ggcgggccgc cgggggcgcg cggcggcgaa ccgcgccaga
     5041 tcgacgccgt tgtagacgac cggcacgcgt tcggggggga ggttcagcgt ggccattacg
     5101 tcgcggcgga ctttctcgct gacggcgatc acgccggtgc gccagcggca gagccagcga
     5161 tcgatccaga cctggcggcg cgtctcccag gtgccgacgt tgtggacctg ggcccagacg
     5221 accggggtgc gggcgcggcg cgccgccgcc gtcgccggca cattgctccg gtacatgtgg
     5281 ctgtgaacga tatcgattcg gcgctcgcgc atcaggtcgg tcagttggcg cagggcgcgc
     5341 ggatcccacc gcttacggaa ggggatacag tcgacgcgga tgccggcggc ttcgagctcg
     5401 tcggccagcg ccccgcgctc gcggatgcag acgagcgaca gctcaaaccg ttcgcggtcg
     5461 aagcgcggca gcgtggcgac cagccggcgt tcgatgccgc cgaccggcag ccaggtaatg
     5521 acgcgcagga cgcgcagcgg tcgatccggc ttcatccgat cgctcgtcgg tgggggttgc
     5581 ggcctccccg ttggccgagg aggatggggt aggagcagcc taacgttaaa ccggcggtgt
     5641 tccaaggccg gatcttgggg attttcgaga gacggatttt gggatgggcc gttgagggcg
     5701 ggcacgaggg cgggcacgag gacgagggac gaattgggat ggaggggccg ggatgctcag
     5761 gcgcgaactc gggctgatgg acgccgtgat gatgggactg gggtcgatcc tggggaccgg
     5821 gatcttcgtc agcatcggcc tggcggcggg gatcgccggt ccggcggtga tcgtcgccct
     5881 ggcgctggcg gcggtgctgg cggcgttcaa cgcgctgtcg tcggcgcagc tcgccgccgc
     5941 gcatccggtc agcggcggca cctacgaata cggctacgaa ttcctgcggc cgtcgctggg
     6001 cttcacggcc ggctggatgt tcgtgctggc caagtcggcc tcggcggcga cggccgcact
     6061 cggctttgcc ggctacttga ccgcgtcgac cggcctgttc gcgggcggaa gacccgtggt
     6121 cgcctgcgtg acggtcgcgc tgctgaccgg cgtcgtcctc tcgggcatgc gccggtcgag
     6181 ccgactgaac atcgcgatcg tggcgatcac gctgctgtcg ctcatcgcgt tcaccctcgc
     6241 gggcctggga gtgcaggcgg cccggggcgg gacggaattc tacactccct tcttcgcggg
     6301 cgacggcgcc ggcgaggtcg cggccaatct gctgcacgcc tgcgcgctga tgttcgtggc
     6361 ctacaccggc tacggccgca tcgccacgct ggcggaggaa gtcgccgatc cccagcggac
     6421 catcccgcgg gcgatcatcg caacgctgct tgtcgccggc gtgatttacg tcggcgtggc
     6481 ggcggcgggc gtcggttcgg tcggttcggc gtttctccag cgggcggcgg cgggcgaggc
     6541 ggcgccgctc gcgctggcgg cgcgccggtt cggcatcccg ctgctgccgg cgctggtcgc
     6601 cgtcggcgcg gcgacggcga tgctcggcgt gctgctcaat ctgctgctgg ggttgtcgcg
     6661 ggtgctgctg gcgatggggc ggcggggcga cgcgcctcgc gcgctggcgc ggctggatcg
     6721 gtccggcgcc acgcccgtcg ccgccgtcat cgccgtcggc gggctgatcg cggggctgac
     6781 gctgatcggc agcgtccgga ccacctggtc gttcagcgcg ttcacggtgc tgatctacta
     6841 tgcgatcacg aacgccgcgg cgctggcgct gccgccgccg gcgcggctgt tcccgcgctg
     6901 gatggccgcg gccggactgg cgggctgcct gttcctggcg ttctgggttg aacagcggat
     6961 ctggctgagc ggtctgctgc tgatcgccgc cggcttgggc tggcatcggc tggcgcggag
     7021 gctgccaaga tgatccgccg cgatgttttc ggcgtgatcg atcagggcgc ctcgcggtcg
     7081 ggtgggtcgg atgcgcgtcc gttgcgcaaa agatcggccc tgtgggataa ttcagagcga
     7141 ggtgaaacat ggtcgatctg catccggaat acgtggttga tgcgcagcgc cgccccaagg
     7201 ccgttctgct gccgctggcc gagtgggagc gaattctgga ggcgctggag gaccttgaag
     7261 atctccgcgc ctatgacgaa gcccggaacc aggctcagga tgcgttgccg tttgatcagg
     7321 ccgtgcgcga gattcgccag gggcgggttg agtgaccgcg tatcagattg aaatccttcg
     7381 ctcggctcaa tagcgattgg cgaggatcga tcgagagaat cagaagcgca tcatcgaggc
     7441 tatttaccaa ctcgccgaat gcccgcgccc ttctggctgc aagaagctca tcggtcgaga
     7501 tgcttggaga gtccgggttg ggacctaccg catcatctac gagattcgtg atgaccgatt
     7561 actgatcctg gtcattgcga ttggggatcg aaaggatatc tatcgccagt ctttcggttc
     7621 gtgaagtatt ccctacttgt tccttctggg actgagatcc gtccgtacgg tcctcgccat
     7681 ggcttgttaa tcgcccgagc gggtttttcg cgttcgaatt tcgcgccggc gcggcttaga
     7741 cttgggggct ggccggggcg gagcaagcgg tgcgccgcgc cttcccggcc ggatccagcg
     7801 tgcgcagcgg agggcaacat gatcgtcggc gtgaccggtt ttttctgctc gggcaaggat
     7861 accctggcgg cggtgctgca ggccaagggg ttcgagcaca tctcgctgtc ggacatgctg
     7921 cgcgaggagc tgcgcgagcg cggcgaggcg atcacgatcg ccaatctgac gcgcgtgggc
     7981 aacgaactgc ggcgcgaagc cggcccgggc gtgttggccg cgcgcgcgct ggagcggatg
     8041 gattacgccc gcaactgggt cgtcacgtcg atccgccacc cggcggagat cgccaatctg
     8101 cgccaacgcc ccgatttcgt gatggtcttc gtcgaggccg accagcggct gcggttcgag
     8161 cgctcgctca gccgggggcg cgagggggac ccgccgacgt tcgaggactt cgcggcggcg
     8221 gaagatcagc agatgcgccc cgaggggggc gatccggcgg cgcaggcgct ggccgcctgc
     8281 cgcgaactgg ccgacgatcg gatcgagaac aacgccacgc tggaggaatt ccgcgaacgg
     8341 gtcgtcaaat tcctcggccg ggcgctgttc gagtacttcc tgccgcggcc gtcgtgggat
     8401 gagtatttca tgatgatggc cgaggtggcg gcgatgcgcg gcaactgcgt caagcggcgc
     8461 gtcggggcgg tcatcgtgct ggagcgccag atcctgtcga ccggctacaa cggcacgccc
     8521 aaggggatca ccaactgctg cgacgggggc tgcccgcgag ggtcgtcggt ggccgattcg
     8581 gggggctcgc tggaggcctg cctgggggtc cacgccgagg agaacgcgct gatccaggcg
     8641 gcggcgcacg gcgtgcggat ccgcggggcg acgctctact gcacgttgtg cccttgctcc
     8701 tactgcgcca agtcgatcat caatgccggc atcgcggagg tggtctaccg cgacgtctac
     8761 gcgatggacg acgtgacggc gcggctgttt caggaggcgg gggtcaagct gcggcggatg
     8821 gatccggcgg aggtgtcggt gcacccgcgc tatgcccgcc gggagcgggg cagggcgccg
     8881 ttgcggcagg gcctgctgga gatttgattc ccggcgtcct ggcgtctcgc cgcggggctt
     8941 ccagttgcga taaggatctt cgccggcccg gctgagggga gcgccgaaag gagccggttt
     9001 ctgaaccagc agaaaatggt ctacgcggag acaggttgtg ggacgcgggg aaggaatcgc
     9061 gggttttcga gagccgagga tgagggacga gagccgggag ccgaattccg aggcgggccg
     9121 ttggggggcg atcacgagga cgagggacga ctcgtgacgg ggggcaagaa cgggcttgca
     9181 agcggtcggg cggggacata tgatttgtag atatgaatat ctattgaacg cgccgcgatg
     9241 acccctcccg aagcaccgcc cctcccctcg acgctcccgg cgacgctgat ggcctacctg
     9301 cgccagcagc cccgggcggt gcgcatgttt aaccgccgcc tggaggtgct gtgggacaac
     9361 tgtcccgacc gcgccgcgga attcccgctc tttaccggcc cgaccggcgc cggggttacg
     9421 gttccgccgc cggggccgga acgcaatgcc tggccggtcc agcgcgtcct ggagcacggc
     9481 cagccggccg aacggttcta tgttcccgcc gggcccgatg cgccggccgg gacctgtttt
     9541 cggctgcggg cgtggccgct ggagtccgac gcgggcgagt tcgagctggt cgtggaggaa
     9601 atcgaccgcg tcaacagccg gatctgcttt gacgaacggc tcaagacgct ggacgaagaa
     9661 ctggccgagc tgatcgatca cgtggcggcg gtcctctcgg gcgaggaggg ggcccatgcg
     9721 ctgcgcctgc ggctgaccaa cccgaacctg cagccgtgcc acgagatcaa gcagtgcgaa
     9781 cgggagacct gcccggctta cgcgaacgag gaaaacccgc gttgctggga tgtggacaac
     9841 acgctgtgcc acaaggggat ggaagttcag aacccgctgg ggaaattccg ctattgcgac
     9901 cagtgcaagg tctacctgct ggcctgcccg gatccgttga cgcgtgtggg ggagaatttc
     9961 aaccggctgc tgtcgctgct taatctgaag caccaggagg cgctcgaggc gcagcagcac
    10021 gtgcagcagg ccgacaagct ggcgacggtg ggcgagctga tgctcggcct ggcgcacgag
    10081 gtcaagacgc cgctgagcgt gatcatcggg cggttggact gcctggcgct ggagctggaa
    10141 agtctgacgc tgcgcgagat cggtgaagat ctggaggtga tgcgccagca cgcccagcgg
    10201 atgagcacgg tgctgaagga cgtgctgacg ctggcgcggc cggcgccgcc cagcccgcgg
    10261 gcgctggatc tggcgacgag cgtgcgcgag gcgctggcga tggcgacgac gacgctggag
    10321 cgggcgcgga tccgggtgga gaacgaggtc ccgaccggcc tgccgacggt gttcgccgac
    10381 ccgctccaga tccagcaggt gctgctcaac ctgatcatca acgcgcgcga cgcgatgccc
    10441 gacgggggcg tgctgcggct ggagacgagg gttgccgagg gagaggccga cgtgctggaa
    10501 ttgcacgttc gcgacagcgg ccacgggatc gcgccggagg accagcagcg cgtgttttcg
    10561 ccgttttaca ccacgaaagc ggagcgcggg gggacggggc tgggcctggc cgtgtgccag
    10621 cggatcatga tgcatcatgg cggccggatc cgggtggaga gccggccggg agcggggacg
    10681 acgatcgtgc tgtcgttctg catccacgag ggggccgtgc catgagcggg gcgacgctgg
    10741 gctcgccgag ccggatcctg gtgatcgacg acgaagtcga catgctgacc aactaccggc
    10801 ggatgctgga gcgggccggg cacgattgtc ggctcgaatc cgacccgcgg cggctggagc
    10861 cgctgctcag tgagtaccag cccgacctgg tgctgaccga tctggtgatg ccgcatggct
    10921 cggggatgga ggtgctggac cgggtgcggc agttcgatcc gacgctgccg gtgatcctgc
    10981 tgacggcgca cgggtcgatc gagagcgcgg tggaggcgat gaagcgccat gcgactgatt
    11041 acctgaccaa gccgttttcg atcgaggaac tgctgggcaa gatccagacg gcgttgagcc
    11101 gccggttgat cgagcagagc gacgagccgg gggcgtcggg gccgccgccg gcgggcaacg
    11161 agggctggcg ccgcgagatc atcgggatca gcccggcgat cgagaaggtg atcacgctgg
    11221 cgcgcaaggt ctcgcgcacc gacgtcaacg tgctgatcca gggcgaaagc ggtacaggca
    11281 aggaggtctt cgcgcggctg atccaccggc tcagcccgcg cagccaggag atcctggtgc
    11341 cggccgattg cgccagcctg ccggagaacc tgctcgagag cgagctgttc ggctaccgga
    11401 agggggcgtt caccggcgcg cactcggaca agatgggcct gttcgagttc gcgcacaagg
    11461 ggacgctgct gctggacgag atcggcgaga tgccgctggc gctgcaggcg aagctgctgc
    11521 gcgtgttgca ggagcgccgg ttccgcccga tcggcgggcg cgagcagatc gaggtggacg
    11581 tgcgggtgat cgcggcgacc aatcgcgacc tggcgcaggc gctggccgag aagacgttcc
    11641 gcagcgatct gtattaccgg ttgaacgtcg tgacgctgcg gctgccgccg ctgcgcgagc
    11701 ggctggagga cgtgccgctg ctggcgcggc atttcctgag ccagttcgcg cgcgacaacc
    11761 gcctggcggt cgatgcgatc acgccgcgcg cgctggagct gctcaaggct tacgattggc
    11821 cgggcaacgt gcgccaactg cagaacgtcg tggagcacgc cgcgacgatg gcgggcgagg
    11881 gggcgatcgg gccggagcat ctgcccgagg agttcgagcc gctgcgcgcg gcggaacggg
    11941 ccgcccgcga gggggacgag gccagcctgt tctcgcgcaa gaccgagctg gtcgatcatt
    12001 tcgagcggga ctatctggtg tcgctcctga tcgaccagcg gttcaatatc tcgcgggcgg
    12061 cgacggtggc ggggtgccat cgccggacgc tgtatcggat gattcatcgc caccggatca
    12121 acctggcgga gctgcgccgg cagcacccgg cctgaggcaa acgtgtcaca gcgcgccgtt
    12181 cgggagggat cggccgggag ggaggaagaa gggagcggag cggaaaaaca ggcggaaaaa
    12241 cggccatatt tcaatcatta agatatgatt aacctaaaca ggcgcggttg ttgcgtgccg
    12301 ttcgggcggc ggtctccgac ccgacgcatc ctgcatgatc atcctcgacc gggccggacc
    12361 gccttgaagt gctgctggaa gagaagaacg accgaggacg agcacgaaga ccaggacgcg
    12421 ggacgacctt ttgagagggg agagagcctg atgaagcctg ccgggccact cgcagcgagt
    12481 catttcaaat ttgtcgggat ctgtctggtc gcggcgctga cggccgcggg cctgccggtc
    12541 ggggcgcagc acgcggccgg accgcccgtg attgatgtgg ttggtgttac gccccggcag
    12601 gtcgccatcg cgccggacga cgtctacgat gaagtcgcct cggggctgga tgtcgtcggc
    12661 gtggggaaca aagtttatct cgaggcgcac accgccgagg acgggttgac gttcagctgg
    12721 tcgatcgtca gccgcccggc ggacagcaca gcccagttga gcgcaaccag cggcaagctg
    12781 gtcacgttcc ggcctgacaa ggtcggccca tacgtcgttc ggctgacgtc gaccgacggc
    12841 ggcgggcagg cgctccaacc cgacgagcgg cggatcctgg cggccaactg ggcgggagcg
    12901 ggcctgttcg atacgcacaa cccgccggcg ccgcgggcgc ccgagtgcgg tgccgggttc
    12961 tgccacgggg gcaacaacgc caagccggaa ttgaacgtcg cgcccgaatg gatcaagtcc
    13021 aaccacgccc aggcgctcca gcttcggctg aagggggaac gcggcgcgca ttacgacgtc
    13081 tcgtgcctgc cgtgtcacac cgtcggctgg aaccagaacg cggcggcggt caaccatggg
    13141 ttcgacgatg tggcgttcga aatcggttac gacgtggcgc agatcccggc gctggttcac
    13201 gcggcggccg agcagggcct ggacaacttc cccgagctgc ccgccgcgct gcaggacatg
    13261 tcgtcgatcc agtgcgagag ctgccatggg gccggctcgc agcatcccag ccggctcggc
    13321 gaggagggca agggcattgc cggcgccgac ctgggctacg agcagtgcgc ccagtgccat
    13381 gattcggcca ccggattcca gcagggcttc taccagtgga gtcagtcgca gcatccgatc
    13441 acgcaagaag cgggcgaggg gcacgtgcag gacaacgcca cctgcctgcc gtgccacacc
    13501 ggcgaggggt tcgtccagaa gatggtgaac aacgagacgc cggcgctggg gcaggcgttc
    13561 cacgggatca cctgcgcggc gtgccacgac ccgcattaca gcgagaatcc acatcagttg
    13621 cggctgacgg gcgacatgac gttcccttcg ggccagcaga ccagcgcggt ggcagggagc
    13681 atggtcggca tgggcgggct gtgcatgcgc tgccacaatt cgcgcgtcag caacctggcc
    13741 ggcacggtgc tgggcagctc gcgcggcgcg cactacggga cgcagggcga catgctgatg
    13801 gggatcaacg gttgggactt cggcctgccg ttcaacgcca gctcggcgca catccgggtc
    13861 gtggaagaca cgtgcgtggc ctgccacatg gccgagccga ccgagagcgg cccgggcgtg
    13921 atcacgccgc cggcggtggg ggcgcactcc ttcgcgatgc gcgacacgat gggcacggcg
    13981 gaccccgccg acgatacgac caatgcggtc aacgcctgtt cgggctgcca cgaggctctg
    14041 gacacgtacg accggcgggc gcggggcgac tacgacggcg acggcgagcg cgaggggatc
    14101 cagagcgaag tgcagggcct gctggcgttg ctgcgaccgg ggttgctgga gttccccggt
    14161 gtgagcgagg aggggccgtt cgaaaagatc aacatcgacg cggctggctt caacctgttg
    14221 acgaacgacc agcgggcggc gctctacaac tacaacttcg ttgtgctcga cggcagcctg
    14281 ggggtgcaca acacggccta cgcggtgcag ttgctccagc gcgcgtacta cggcgtctac
    14341 ggccgtccga tcagcaacga ctatccggcc atggcgctgc gcgggccggt gcaacagaac
    14401 agcgcctcgg ggtgggcgga ttaccgctga ccgcgggcac aagagggatg ggctgcatgg
    14461 cctcgccgga caacagggtc catcagtcaa gtgcgtcggc cggccatgca gcccggggtc
    14521 cggtggtatg gattatcacg ccccaccgga cggttcacca gcggatcgaa cgcctgttgc
    14581 ggccgctgcc ggccgaggtg gtccggcggg agcgggtgtt ggaagacccg cggggaagtg
    14641 cccggccgaa accacggccg caactggtgg tgctggacgt ggacgaggac atggagcagg
    14701 gggcgcgggt gattgaggcg ctacggcgcg ggcggctgga cgcgccgctg atcgtgctga
    14761 cgcgcgcgtt cacgcgcgac ttcggggcgc gcatcctgtc gctcggcgtc cgctactatt
    14821 tctcgcagga tttctgcgag agcgagttcc gggaggtggt gcagagcctg ctgcgcctgc
    14881 cggcgagcga ggtgtgaacg cggagggcgc cgctcgcttt ccgcccatca tccaattcga
    14941 aagaaaggcc ttgccaacca tgaagccact gcgcaaggga cagaggcggc gcccggccgg
    15001 ccggccgcgg cggatgatca cggggctgct gctggcggcg gcgatcgccg tggtgttgct
    15061 cagcgtgcag ccggccccgg cgacgatccg cggttcggcg cacgatttct ccggcgtcga
    15121 ccccgagcag cagatctgca ttttctgcca tacgacgcac aatgccgaca cgacggtgac
    15181 cgacgccccg ctgtggaatc acgacgtgac gacgaagaac taccaggtct acaacagccc
    15241 gtcgctcgac gcgacgccgg gtcagccggc gggcgcctcg cggctgtgcc tgagctgtca
    15301 cgacgggacg atcgcggtgg actcctacgg cgaccgcacc ggcgtcattt tcctgggggg
    15361 cgacgcggcc atcggcgccg acgagctgac caacgatcac cccgtgtcgt tcacctacga
    15421 cgactcgctg gcggtgcagg atggcgagtt gttcctcccc tcgtcggcgc cgtcggggat
    15481 gggcgggacg atcgcccagg atctgctgtt caacaatcaa ctggagtgcg cttcgtgcca
    15541 cgacgtgcac aatggcccgg cggcggcggc ggtcaacgat cacctgctga ccgtgaccca
    15601 ggttcagagc caactgtgcc tgacgtgtca caataagtga tccgggttcg gcgccggcgg
    15661 gcgcggggcg gcgtttcggg tggatgatgg gaagccaggg gccgggatgg aacggcttgg
    15721 atgaaagccg gcatcccggc cccgctgcgt tgaacggggt ggaggagggg gattgcgagc
    15781 cgaggacgag agccgagagc cgagagccga gagccgaatc tattggtatg cctttggggg
    15841 cgagggcggg gacgagggga gcgaaaaatc ctgacacaca gctatttaaa gatgtatgga
    15901 tcttttggta tggttattgt atagaattcg agatggcgac atcgcttatt cccggggcgg
    15961 ccccatgatc ccacgactct ccactctccg actccggcct tgcctcctgc cggcgctggc
    16021 gctgctgctg agcctggcgg gctgcgcgcg ggcgccgcgg gtcgagccgg cgccggggcc
    16081 gctcttcttt ccgccgccgc ctctgccggc gcgcgtgcag tttctcggtt cggtctcctc
    16141 gccgctggat ctgccgcggc cgcgcagcgg gttcgccgat ttcattctgg ggccgccgcc
    16201 gctcaagtac cccttggtca agccgttggc ggcggcgctg cgcgacggca aactgtatgt
    16261 ctgcgactcg gtcctcaaca ccgtcatcat ctacaacctg ctgaccggcg aagcgggcct
    16321 gctgccgggc gacgcgggca acggcaagct gcagcaggcg aacaacctgg aattcgacga
    16381 gcagggcaac ctctacgtgg cggacaagaa gcgccaggcc gtgctggttt atgatgcgcg
    16441 cggcgccttt gtgcgggcgg tggggaggcc gggcgaggcc gaaccggtcg acatggcgcc
    16501 ggccggcggg ctgctctacg tctgcgacat caaggatcat cagattgagg tgtgggacgc
    16561 ggccagcggc gcgctcgtgc gccagttcgg ttccaagggc aacgggaagg gggaattcta
    16621 cctgccgtcg caggtggcgc tgggtcccga cggcaacctg tatgtgaccg acacggggaa
    16681 tttccgggtt caaaaactga cgccggaggg ggaattcctc gcgacggtgg gggggcacgg
    16741 cacggcgctg gggaatttcg cctggcccaa gggcctgggc ctcgatggcg acgggcggct
    16801 gtatgtggcc gattcgcgct tcgccaacgt gcagatcttt gatcccgagg ggcgcttgct
    16861 gctgtttttc ggcgggccgg gtcccacgcg cggccatctg gacctgccgg ccgggttgag
    16921 cgtgatgccc tggccggagg cgccctggct gcgcgaacgg ctcgccgccg gattcgtccc
    16981 ggagtggctg gtgatcgtcg tcagccagaa gggcgacggg atggtgaatt ttttcgcggt
    17041 ggcatccgac gatgcgccgg cgccatgaac cgggcgctcc cccgcctggc gtcgtcccgc
    17101 ctggcgctgg cggggctggc cctgctgttg gcgtcgtgcg cgccgccgcg gcggcaggag
    17161 gtgctgtcgt tttttttcga cggcgtgccc ggcgcggggg aggcgggcgc gccggccggc
    17221 gcggcggacg gactcagtcc gctgttccac cccgagccgg ccgaggcgct atcggcgccg
    17281 gcggcgccgc cggtcggctc ggttcatgcg ccggttttcc agcgccagtg caacacgtgc
    17341 cacgacccga ccagcgggct ggcgccgcgg ggcaaggatg ccgcgttgtg cgaccgctgc
    17401 caccaggagc agcgcgagcg cgaggggtgg aaccatggac cgatcaacct ggggacgtgc
    17461 atcccgtgcc accgggcgca cgaatcgccc tacccgcacc tgctcgacaa gccgctgccc
    17521 gagctgtgcc tggactgcca caaggaggac ttgagccggc cgagcgaata tcacagcgag
    17581 acggaagtgg aggcgtgcac cgtgtgccat gacccgcacc ggatgtatta ggcgggggag
    17641 cgagccataa agcgcggtct tatgcggaat tttcgagagc cagggacgag ggacgagagc
    17701 cgagggacga acgtgagggg gactgagaga tgacgttgcg atccgctccc atccgccgcg
    17761 cggtggcgct ctgcctgggg ctgatcggcc tggcgggggg gggcgagcgc gcgggggcgg
    17821 ggatggccga cagccagtgc ctgaagtgcc atggcgaggc atggatcgcc gatgcgcggc
    17881 cggaggcgct ggccgcgatg gtgcgtcacc ccgacggcca gccggtgctg cgcgcggagg
    17941 aggcgatcga cgaactctac acgccggccg cgatcttcgc cgggtcggtc cacgccggcc
    18001 tgagctgcac cgattgccac cagaccggcg accggctgcc ccacgaccag cggctgccgg
    18061 agatcgcctg cggcgattgc cattccgagg tagaagacgc gctgcgggcg agtgcgcacg
    18121 gcggcgcggc gcaacagggc cgcgcccgtc ccacctgcac cgactgccat ggcccggcgc
    18181 acgagatcca ggcgttcggc gagccgcgga cgcacgccgc cgccgtgcgc gaaacgctgg
    18241 cctgcatgga gtgccaccgc gacggcgacg acgccgaggc gcacccggcg gccacctacc
    18301 gcgagaacgt ccacggccgg gcgctgttcg agaaagggtt gtcgatctcg gcgacctgcg
    18361 tggattgcca cggcgcgcac ggcgtgctgc cggcggcgga tcccgccagc ctgatgcatc
    18421 cgcgcaacgt gccgcaaacg tgcagccgct gccacgaggg gatcgccgag gtctactacg
    18481 gcagcgtgca tggccgccat ctgctggaag gcgatgagac ggcggccacc tgcaccagtt
    18541 gccacggcag ccacggcgtc gaggcggtgg ccgatcattt tctgctcggc gtgatcaacg
    18601 aatgctcgca ctgccacatc gacctgggcc ggagctacct gaccagctac cacggcaagg
    18661 cggcgcatct gggttacgcc gaggcggcgg tctgttcgag ctgccacggc gcgcacgaca
    18721 tcctgccgcc ggaggacccc gcctcgcggg tggccgaggc caatctggtg gcgacctgcg
    18781 gcgcctgcca cgagggggtc aacgccaact tcattcagtt cgacccgcac gtcgacctgc
    18841 gcgaccgtgc ggccaatccg gaggtgttct ggacgtggct ggtgatgacg acgctgctgc
    18901 tgggcacgct ggccgtgttc attccgcacg ggctgctctg gtttcagcgc acgctcgtgg
    18961 aacgcctgcg ccatccgcgc ggtttccacg gcgtgatgca cgaggagcgc tgggtgatgc
    19021 ggttcaatcc ggtccaccgc atcacccacg cgctgatcat catcagcttc atggggctgg
    19081 tgctgacggg tttcccgctc aagtacagtc atacggagtg ggcgctgggg atcacgcggc
    19141 tgttcggcgg catcccgatg atggggctgc tgcaccgcgc gttcgcgctg atcacgttcg
    19201 cctacgtcgg cgtgcacgtc gcattcctcg tcatcttctt ctggaaacac tgcccgcggc
    19261 cgcgccggcg ctacctgttc ggccccgatt cgatggtctt ctcgctgcgg gatctgaagg
    19321 acttcatcgc gatggtccgc tggttcctgt ggctggggcc gcggccgaag ttcgaccgct
    19381 gggcctattt cgaaaagttc gattactggg gcgagatctg gggggtgatc gtgatcggcg
    19441 gcaccgggct gatgctgtgg ctgcccgagt ttttcacgcg ctggctgtcc ggctgggtgc
    19501 tcaactgcgc gatggtggtc cacagcatcg aggcgctgct ggccgcgtcg gtcatcttcc
    19561 tggtccattt cttcaatacg catgtccggc cggagaagtt cccgatcgac atggtgatgc
    19621 tgaccgggcg gatgaccgag tcggagatga aggaggaacg gggggacgaa tacgcccggc
    19681 tggtgcagtc gggcgaactg gagcggcggc tcgtgccgcc ggtggcgctc aagtggcggc
    19741 tgctgggggc ggtggccggg ctgatggcgt tcgcgctcgg cgtgacgctg atcgtgctgg
    19801 cgatcacgac ggagtttgac cagatcttcg gatgaaaagg tggtgatcat catgcagcgc
    19861 gggagacggg ggatggcgat gaagcaagaa cgcggggcgc ggagcgaccg gcggcgcggg
    19921 cgggggctcg cgccgcggct ggcg
//
LOCUS       DAOPCO010000042        19834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2845_length_19834_cov_4.747207, whole genome shotgun sequence.
ACCESSION   DAOPCO010000042 DAOPCO010000000
VERSION     DAOPCO010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 19834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19834
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_19834_cov_4.747207"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..410)
                     /locus_tag="PLS90_05145"
     CDS             complement(129..410)
                     /locus_tag="PLS90_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPK01822.1"
                     /translation="MHPAARREIRRAFEWYLQEASARIATGFLDDFEDTLALLKTHPE
                     IGEPGGSNTRRLIFQRYPYTVVYRSKGNTLEIVAIAHHSRQPEYWAGRL"
     gene            complement(422..1087)
                     /locus_tag="PLS90_05150"
     CDS             complement(422..1087)
                     /locus_tag="PLS90_05150"
                     /inference="COORDINATES: protein motif:HMM:NF023219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01823.1"
                     /translation="MNDAYRQHVAERAEQGIPPLPLNAEQAAEVCRLLTSAAPADAEQ
                     LLHLLVERVPPGVDPAAQVKATFLARIAKREAQCPLIDPREAVRLLGTMLGGYNLNFL
                     IEFLSVPDLAETAADALSHTLLIFDHYHEVLDLANAGNAAARRVIDSWAAAQWFLDKP
                     ELPEAIDLTVYKVDGEINTDDLSPAVHAVSRADIPRHALAMLEQRDPEARARIAELRA
                     RLP"
     gene            1442..2530
                     /locus_tag="PLS90_05155"
     CDS             1442..2530
                     /locus_tag="PLS90_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01824.1"
                     /translation="MLAIALLAPAPGAQESAPARRPDPPISGQQAFDSVPEAAAPSDG
                     PPAPTHPLLAPVEAGAPELAWLQERRRWLAHQPADVAVYRQVAEQLAERKAWFPAIEI
                     LWFAEKIAQDAATREALNRRMVELEGQTREVGQRIDTAVQLWGAGHQPDALATLQKIR
                     DEYPYAEQAYYHSGLLLNQTYQQQVGKAEEAEQLPPLEIRRQLFQVIHRLLASTILID
                     PLFYDAYYQLSLLRSLMPDDAEFVRQSDVLSQRAMTFYSQLMPAMEVLDGGDRSAAAL
                     ARAAEAFTQIGAGEYAVYAWQAALAQPDITPELAAQGGGAIERIRAEGLGGAGGDGGV
                     GGAPSEGRSDEAEADGETSSTPAQPVLP"
     gene            3727..4125
                     /gene="speD"
                     /locus_tag="PLS90_05160"
     CDS             3727..4125
                     /gene="speD"
                     /locus_tag="PLS90_05160"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPK01825.1"
                     /translation="MQRGQHLLVDCRNVNSSLCLNDELFLDVMARSARRAGATVISQI
                     RYKFGQDSPPGFTAIVMLDESHVSAHTYADLGLVALDIFTCGHTNPRDVLAAMREELD
                     LGDVEIRAVPRFTTPDPVNSPDGALSGVTN"
     gene            4401..5480
                     /locus_tag="PLS90_05165"
     CDS             4401..5480
                     /locus_tag="PLS90_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPK01826.1"
                     /translation="MDRNALHDGTLDGLTPLESLDLSQIKSFADLLHAMRQTAFAGRQ
                     LGEAFEILVQMMRDAETTVVLTLSGAMTVAKQGRIICELIDRGLVQAIVATGALMAHG
                     LTESIGLTHYQYDPTMDDRELFAKGYNRIYDTLEMEANLNNVERLVREVLDHNAPEDG
                     VWSSARFCRAVGRRLDELDAGVGILRSAYLREVPVFVPAFTDSEMGLDFSTWAMSKAL
                     QGAELESLTPEEIYQAVPSYNPFLDLQEYARLIGRARTLGIFTIGGGVPRNWAQQVAP
                     YFDITNYRIGTGMKAPRFKYGVRICPEPVHWGGLSGCTYSEGVSWGKFMPPEEGGRFA
                     EVMADATAVWPLLMKAVFEELDRQG"
     gene            complement(5708..6703)
                     /locus_tag="PLS90_05170"
     CDS             complement(5708..6703)
                     /locus_tag="PLS90_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK01827.1"
                     /translation="MSPLSLRHRLLIVVWALAASGCVYTPPAPEPGQDPRETVMIVNG
                     LPITRLQLEEEIDAVLAEAPPGIEERELREKAPLIRDEALRRLIFDRIIEAAVAEHGI
                     EVSEAEVDDEIRTIAEYLTAHGRTLPDAMREMGDTTHTLRMAIRRQLTEIKLWDKLLN
                     LQPPTEEELRAYYQAHREWYVEPDAVLIYQIVKFRPEGDDSADADERLRSEMALLRNR
                     WLAGEDFTELARRYSDGAHAAQGGKIGWITADVNLPAEVVEAAFTQQVNQVGEVVQSP
                     MGYHLVWVAARRPRKQLSFEEARPVVEDHFSHERRREPVIQLYEKMRQEATIIEP"
     gene            complement(6866..7480)
                     /locus_tag="PLS90_05175"
     CDS             complement(6866..7480)
                     /locus_tag="PLS90_05175"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01828.1"
                     /translation="MTPHRRHRLRWPLLGLFVLVMVGCLVGSIFFAGQPIRRLYERSQ
                     ADSLLAMAQILKTPLLPLVLVENRWADAEALAQRLGADAGVRITLLLPDGTVIADSEE
                     EPARMGNHGDRPEIRQAILERRGDAVRPSETLGTRMLYVATPLHHGGRMIGVLRLALP
                     LAPIDDALAAHRRLMLRNGLLVTLATALLGLLVVWVFVNHEERR"
     gene            7748..9100
                     /locus_tag="PLS90_05180"
     CDS             7748..9100
                     /locus_tag="PLS90_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01829.1"
                     /translation="MTMHADPSSLRLFPPCHGVLADLQRCGWRPADQPSAPRLEFRTY
                     QGVHEAQCAEVENPAGRRLLLIGLESVVLARDEDLFPRFLNLAIETRMALAYGVAASR
                     ADWLLLLTRDRIDLYRLPEETALQQATAAGDYDEGLLPELAALARTEVDALRLGSQHL
                     RGADSLRGWIRHWALQLAYAVQLPPDQVEPVLWRWILMLQWVRRTERSEANGSWGLYC
                     RAEGDRWAVGYDAQTAFADLTNALSRFDELFHSEMFRCDLELLLGRLNQIEETTLLDR
                     LRAELLMHSQDRFEPETVAWLFTDLAREQEGWRREVSGVEPLRRRLHHQGWTIYRPLA
                     CDVERHGLTFALRETERLAHYLNNQMVYARQPESPSPATAQPDLFKPEIRGVGPTGHL
                     DDPVNYLFEEALRLRGVEAESRFGVGLVFLLKALALGQEFDWPFFGVDTLDALFREAG
                     "
     gene            complement(9382..10716)
                     /locus_tag="PLS90_05185"
     CDS             complement(9382..10716)
                     /locus_tag="PLS90_05185"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK01830.1"
                     /translation="MPESLNPRLLSFYLASLHELGGALARTPRKLDQNKPLRESLYRV
                     LGTFAVGAGMILLWDEDEALLRAAAAKGLRAPAEGLPLKPALARALAAGRRPFHPHLL
                     PGGLADAEPLFRELIGRRNIQLIVPLTSGHEFAGLLLLGPRVNGRPLSPLETDVLTEM
                     AGVMALRITDARARRRLDRQLHETRRLNRELRRIYLETVRALASVIDGPEQDHRPGHS
                     ARVAALAAQIGRRLGLDRDRCEALYLAGLLHDVGKQIIDRDLLRKSGPLAPGERARVE
                     EHAAAGYELISHLRFPWGDVAEIIRHHHERLDGTGYPDRLRGDELSIEARVLMMAEAF
                     DSMASDQPWRPRLTIERIVEQIEANLGLQFEPRVVQALCDAVQDGLESPDGAAGEAAV
                     FGAPGEPEFVPHLRASFDPALIHQMLGELRRRLNNPRLNRTARVIEIVEPAG"
     gene            complement(10765..11157)
                     /locus_tag="PLS90_05190"
     CDS             complement(10765..11157)
                     /locus_tag="PLS90_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK01831.1"
                     /translation="MASPETQLTIAVQREADHVVIVPTGYLNALTGERIDKVCDALLK
                     EGIKYILINFKQVELINTIGISILVGIIEKVHLQGGLVYFTHLGGTNLEIFEVLDLGS
                     VALFFPTDDQAREHLRHDRETQRRALGQ"
     gene            complement(11282..13684)
                     /locus_tag="PLS90_05195"
     CDS             complement(11282..13684)
                     /locus_tag="PLS90_05195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01832.1"
                     /translation="MAMPDSTRPWRDLTAARPREEFAGRRDLLLRLRLWARRADGRGP
                     DALGLTGPAGCGRSEILRQLTLDLAARETPIPFYFLQAEAPGGSTPGALAITSPVGWL
                     SLLRALIRQTLFRQGRLEADERVWLRRDPSHLSGICHGAELHSLAELTLTAAPDDAEL
                     GWEAVLAALAADLSAPPLLIVDGLAPLPWPEARAALMPLVRAALRAGADLLLEGPESP
                     EDWPAADAFQWLAVPPLNEEESLALISAAAVRANWALPLPACRPVVRRLGGRAAWLHA
                     WAASVEPGAEEDDPHRRAHLAYVDLACGSHWANRLMQQFQHVVRLNLREPALRLIQRA
                     VQLDRPLSADEVLAFLPCSPAEAERILEGIGRLGLLTRRGARWGAPAIPLLADWAHLY
                     LTAGADTDALPAERLRMLSALLRTEVPAPEAPPAAARFPDARALLAEFRGQSVPEMLF
                     QFADFSEAAGRMTLAEARELAASAAAAMPLPEILGVAPWQPAGWSERHRRQAPPVYYA
                     HGYRGGKYQRSHEEVWLLFDLRAVSVLTSVEIDRALGAARAFERRMGPGRYRLWLLVG
                     KMASPEAMERLRQEQILASGPTQIELLVDLLKRARRGDGDPSAAAATPADLPPDETAD
                     PAGESASAPAPPGRPGGVIVLGHSRSRPENEISQLSLPARSESEVIAAMMAEKVALQA
                     GLDPAAAGQVKTAVLEGVLNAIEHSPSPEKMVELQFAVDPLALEVVIQNEGTEFDPLA
                     IPEPNARRKLETENKRGWGIRLMQRFMDEVGYEPFPGGTRLRLVKRRPAPRGRRAEST
                     NA"
     gene            complement(13981..15642)
                     /locus_tag="PLS90_05200"
     CDS             complement(13981..15642)
                     /locus_tag="PLS90_05200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPK01833.1"
                     /translation="MLTLDKAPASTPASGAFRAAQPDPTDNDPPVGLTELTTFTLRHR
                     SGLEQIALANENPLCRLLARTREGERVCNRGCGIDPAAPPDRHVCPFGMAMQRLAPAG
                     APDPRLTSRWIGRRFLSLQSLHAALDRLLAEGLDEETIFAHLPSNPVVLIHELRRAAG
                     DAARETDHEPSTDLEIPPPEPAGAAAPTRQPDEPEFRPSSELTHLLEFVLQLQTLLAS
                     SDQPGSICERFLRALGGLLPFDAMSIYLRESVDAEPVLVSTVEREPADAAQAPSPRAH
                     DTRLPAHAIGALALGERRVMIQQGDTVRRYAGEFRGPGRVALPFPLSRGEAMGVWLAE
                     IDHEPRDAAAEWQRDAPLWMQVLAEVLAARLAQVPGNPEPARRRETPAPAFAAPPAPA
                     PPACPRDGLARRLAVESVRCARHGYGFSLVLVGVAFEAERVALPEAELSDQFRAALRP
                     YDECFRLDGGDAVWAVLLPHTSEHEARRVAFRLISIVEDVLDAHGGAEEMGIRPGAGI
                     SLWGVDASSADQLIHQAEAALARALACTHEPVIQVFGHGEDAEVG"
     gene            16174..17268
                     /locus_tag="PLS90_05205"
     CDS             16174..17268
                     /locus_tag="PLS90_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPK01834.1"
                     /translation="MALNIKALVEGAYTHNASDIHVVQDSPTFVRIDGHMRPVSNTHV
                     TKEDIEELLRQIMPAEVSGDLQQRRGADFAWQPDERMRFRVSAYYERDRLRLVMRLIR
                     IRIATIDDLGLPEVLKTIASWHRGITLMTGVTGSGKSTTLAAMIDYINSRESRCIITI
                     EDPIEMVHTNKKSLISQREVGRDVESFRHGLVQALRQDPDVILIGEMRDPETISTALR
                     AAETGHYVFSTMHTSNALHTVERILSEFSEEEHALLRDQLANNLKATITQRLVRRKGG
                     VGRVAALEIMVVNDMIRKLLLENQIPSIATVIRERRDGNVLFDQFLADLVRSETIDEA
                     EAYQYVEDEAAFRRYVKGRLATADRGGIIG"
     gene            17261..18955
                     /locus_tag="PLS90_05210"
     CDS             17261..18955
                     /locus_tag="PLS90_05210"
                     /inference="COORDINATES: protein motif:HMM:NF018455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HPK01835.1"
                     /translation="MAEHITTTPARLRTGVAGLDELLGGGLLAGRTVLLKGSPGSGKT
                     TLGLQMLIAGATQFNEPGILVTFEQAPEQLYADTAGFGWDLRRLGEEGKVQTVFIEPE
                     EMIEHRGRQANRLLVNIADWVDDYGCRRILIDSISHLSTVFTNEEARAGFLKFVMQLK
                     SMGLTPIMTAELDALQGLMGLDAYLVDAVIHLHHEGGSVGTRERRNLEILKTRGYQHV
                     GGRHPLEIGRNGVVVFPHTYPAADAAEAAAAAPVGADAAPVSTGVHALDLLLSGGYRE
                     GSTALIAGLSGTFKSTLAGHFLLGGGGEAPAPGLWLSFHESTADLIQSFGWREMDLAG
                     AVESGRVRVMELAPGQEPVEKIYYRAAALIAEHGIGRVVIDTLGDLTLGLGRAEEARE
                     AERWFLRRLKALGVTTLATQQLVKVTGRNPLSEIGAAELADTIIYLGLVEIESRLEKV
                     MSVLKHRGGPVSGDLRAIACGAGGLQVSERFVGLSGLLDGTARGQRKTQIEEVFQPLY
                     FIRDFLKMASRPDLDPARRGQMLERLEGETSRVIASLAQYFDLPLGETDTRKDSKA"
     gene            18952..19317
                     /locus_tag="PLS90_05215"
     CDS             18952..19317
                     /locus_tag="PLS90_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01836.1"
                     /translation="MSARILIIDDADHLREVLKMTLEFSDYEVAVATNGREALELTQT
                     SRFDLIFCDIDMPVLNGYGFVEGYRAAHGDQTPIIMLTAEDQASINKALALGANAALV
                     KPFEPFQLLDVVKEHVKEG"
BASE COUNT         3189 a         5909 c         7707 g         3029 t
ORIGIN      
        1 aaaggttcac gactcgctac ggaatctgct taaggaagac ggcagtttcg ataaggatgc
       61 attgcgccaa gcgttgcctg gattggagcc ggaagttatt gacggcttct gatgcgcggt
      121 cgccacacct acagccggcc agcccagtat tccggctgcc ggctgtggtg ggcgatggcg
      181 acgatttcga gcgtattgcc ttttgaccga taaaccacgg tgtaggggta gcgctggaaa
      241 atcaggcggc gggtattgct gccgccgggt tcgccgattt ccgggtgggt tttcaggagt
      301 gccagcgtat cttcaaaatc atcgagaaac ccggtagcga tacgtgcact ggcttcctgg
      361 agataccact cgaaagcccg tcggatttcc cgacgggcag ccgggtgcat tcggatggaa
      421 atcacggcaa gcgggcgcgc agttcggcga tgcgggcgcg ggcctcgggg tcgcgctgct
      481 ccagcatcgc cagggcgtgg cgcgggatgt cggcgcgcga gacggcgtgg accgccggcg
      541 agagatcgtc ggtgttgatt tcgccgtcga ccttgtagac ggtcaggtca atcgcctcgg
      601 ggagttcggg cttgtcgagg aaccactggg cggcggccca ggaatcgatc acgcggcggg
      661 cggccgcatt gcccgcgttg gccaggtcga ggacctcgtg atagtggtcg aagatgagca
      721 gggtatggct gagcgcgtcg gcggcggttt cggccagatc ggggacggag agaaactcga
      781 tcaggaaatt gaggttgtag ccgcccagca tcgtgccgag caggcggacg gcctcgcgcg
      841 ggtcgatgag gggacattgc gcctcgcgtt tggcgatgcg cgccaggaag gtggccttga
      901 cctgcgcggc gggatcgacg ccgggcggaa cgcgctcgac gaggaggtgg aggagctgct
      961 ccgcgtcggc gggggccgcg gaggtcaaga ggcggcagac ctcggcggcc tgctcggcgt
     1021 tgagcggcag cggcgggatg ccctgctcgg cgcgttcggc cacgtgctgg cggtaggcgt
     1081 cattcatcgc attgctccct tgtcccatcc ggtctggtcg gcgcgccgaa ggcagatcgc
     1141 agacttacat tatcaggatt cggcgggggg gacaagcccg ggccgggtgg gggaaggtgc
     1201 ggggggctgg cggggtgcgg gggggatgag gcggagatgg gcgcggggcg ggttatgagg
     1261 aaaccgttga aacggtttga tggcgatgac gatacgatgg attctgatgc gttgccgacg
     1321 gtgagagcgc atgttatgct ccccctctca gcttgcggtg cggacagact ggcaacagga
     1381 aggcaggggc ggcagagcgg caatgaatgg cctgaatcga cgtctaggga ttggactgat
     1441 cgtgctggcg atcgcgctgc tcgcccccgc gccgggggca caggaatccg ccccggcgcg
     1501 gcgtccggac ccgccgatct cgggccagca ggcgtttgac tcggtgccgg aagcggctgc
     1561 gccgtcggac ggtccgccgg cgccgacgca tccgttgctt gcgccggtcg aagccggcgc
     1621 gcccgagctg gcctggcttc aggaacggcg ccgctggctg gcgcatcagc cggccgatgt
     1681 cgcggtctac cggcaggtcg ccgagcaact ggccgaacgg aaggcctggt tcccggccat
     1741 tgaaatcctc tggtttgcgg agaaaatcgc gcaggatgcg gcgacgcgcg aggcgctcaa
     1801 ccgccggatg gtggagctgg aggggcagac ccgggaggtc ggccagcgca tcgatacggc
     1861 ggtccagctc tggggggcgg ggcatcaacc cgacgcgctg gcgaccctgc agaagatccg
     1921 cgacgaatac ccctacgccg aacaggccta ctatcacagc gggctgctgc tcaaccagac
     1981 gtatcagcag caggtgggca aggcggagga ggccgaacag ctcccgccgc tggagatccg
     2041 ccggcaactg tttcaggtca tccaccggct gctggcgtcg acgattctga tcgatccgct
     2101 gttctacgac gcctactacc agctgtcgct gttgcgctcg ctgatgccgg acgatgccga
     2161 attcgtgcgg cagagcgatg ttctgtcgca gcgcgcgatg accttttaca gtcagttgat
     2221 gccggcgatg gaagtgctgg atggcggcga ccggtcggcg gcggcgctgg cgcgggcggc
     2281 cgaggcgttc acgcagatcg gcgccgggga atatgccgtc tacgcctggc aggcggcgct
     2341 ggcccagccc gacatcacgc cggagctggc ggcacagggg ggcggcgcca tcgagcggat
     2401 ccgcgcggag gggctggggg gcgcgggggg cgacggcggc gttggcggcg cgccatccga
     2461 agggcgatcc gacgaggctg aggcggacgg tgagacaagc agcacgcccg cccagccggt
     2521 tctgccgtag agggggaagg cggttgatgg gctcaggggg cctccgatgg acggggtgag
     2581 gcggagaatg aaatgagcgg gttgcgggga aaccgttgaa acggttagaa acatttgttg
     2641 taacgagtca ccgggccgcg gcccggtgct aatgacaggg ccccgagggg cccaagaaaa
     2701 gcccgccggc gcgggcggtc agcaagccgc aaacggtagt tatcgaattc cgccccgagg
     2761 agacgaccgc cggggttggc cctggcaaga aggcccgcaa tggcgagtag gcctccaccc
     2821 gatgggacga tctcaagcac tgctggctgg ctgattgttc gcgtctgacc ggtcgcttca
     2881 cgacgccttc gacattaccc tgagaagatg aaaaacaggt ccattcgcgg atgggcgaga
     2941 tgcgcccccc cccgcgggga ggggccgttt tccgccccgc tgaggcagcc catgcggacg
     3001 gatcttgtca gcggaaacag aggggatcgc gaaagacacg aaggggcacg aaagggatgg
     3061 ggatgggaag cgaccggcgg gggcgccggt cccacaccgg ggtggcgcgt cgctcggcta
     3121 tccgctggca tccctgcggg atgcgggggg attcgagagc cgaggacgag ggacgagagc
     3181 cgggagccga gttttgggga gggccgttgg gggcgatgta gctcacgccg aggcgcagag
     3241 acgcggaggg gtgcaggcgc ggaggatgga gaggtggatg gaggggtgga tgggatgagt
     3301 cgggcttgaa aaggggcgac gcgagtcagg agagcggggc tcccagggtc agaagagggg
     3361 cgttgcaagt ctcagaaaaa tcgcgtgttc cgcttcagga aagcggcgtt ccgaggttca
     3421 aaagactggc gtttacgagt tgaagtgagt ggggttcccc agctttgaga ctagaggact
     3481 tcgcggcgaa aaaggggctg gcgcggggac gatttttttt caccaaatgc tgctgatttg
     3541 gcttgcgcga gggcggaact gggttatagt gaatccggct cgcgtcccgc cagtgagggc
     3601 gcggccagtc cagccgcgcc gcaaccgccg cctcagcgtc catgcggcct caccccccct
     3661 gccgcgggca gggccactcg ccagcgtggc ggtggaaacc aattcgaaac aagggggttt
     3721 tatgaggtgc agagaggtca acatctactg gtcgactgcc gaaacgtaaa ttcgtcgctc
     3781 tgcctgaacg acgagctctt cctcgacgtg atggcccgct ccgctcgccg ggccggcgcc
     3841 accgtcatct cccaaatccg ctacaaattc ggtcaggact cgccgccggg attcacggcc
     3901 atcgtgatgc tcgacgaaag tcacgtttcg gcccacacct acgccgatct gggcttggtg
     3961 gcactcgata tcttcacctg tggacacacc aacccgcgcg atgtgcttgc cgccatgcgc
     4021 gaggaattgg atctgggtga cgtcgagatc cgggcggttc cccgatttac cacacccgat
     4081 cccgtcaact cgcccgatgg cgccctgagc ggcgtcacga actaaccgcg agccccgcgg
     4141 ccggaggctc atccgccggc gtgaagcgcc gcggcggcga tggcgacgtg cgtgccatcg
     4201 acgccgggcc agtgcgccgt gcggtccgcg gcggctctcc ttgaagcagc cgggttccct
     4261 cgcgtttgcg aatgcgaggg aaccttttca tttccatcgc gccggcgctg tgctacgggt
     4321 agggccatcg cccgccgacg gggagggcgt tgcccgggcg agtcggcgtc cgacttccaa
     4381 cgagacggag ttcacgcacc atggaccgca atgccttgca cgacgggacc ctggacgggc
     4441 tcacgccgct cgaaagtctg gatctgagcc agatcaaatc gttcgccgat ctgttgcacg
     4501 cgatgcgcca gacggcgttt gccggccgcc agttggggga agcgtttgaa atcctggtcc
     4561 agatgatgcg cgacgccgag acgacggtcg tcctgacgct gtcaggcgcg atgaccgtcg
     4621 ccaagcaggg gcggatcatc tgcgagctga tcgaccgggg gctggtgcag gcgatcgtcg
     4681 cgacgggcgc gctgatggcg cacgggctga ccgagtcgat cgggctgacg cattatcaat
     4741 acgacccgac gatggacgac cgcgagctgt tcgccaaggg atataaccgg atttacgaca
     4801 cgctggagat ggaagccaat ctgaacaatg tcgagcggct cgtgcgggag gtgctggacc
     4861 acaacgcgcc ggaggacggg gtctggtcgt cggcgcggtt ctgccgggcg gtgggccggc
     4921 ggctcgatga gctcgatgcc ggcgtgggca ttctgcgcag cgcctacctg cgcgaggtgc
     4981 cggtgttcgt gccggcgttc accgacagcg agatggggtt ggacttctcg acgtgggcga
     5041 tgagcaaggc gctgcagggg gccgagctgg agagcctgac gccggaggag atttaccagg
     5101 cggtgccgag ctacaatccg ttccttgatt tgcaggagta tgcgcggctg atcggtcgcg
     5161 cccggacgct ggggatcttt acgatcggcg ggggcgtgcc gcgcaactgg gcgcagcagg
     5221 tggccccgta tttcgacatc accaactacc gcatcggcac cgggatgaag gcgccgcggt
     5281 tcaagtacgg ggtgcgcatc tgccccgagc cggtgcattg gggggggctg tcgggctgca
     5341 cgtattcgga gggcgtgagc tggggcaagt tcatgccgcc ggaggagggg gggcgtttcg
     5401 ccgaggtgat ggccgacgcg acggccgtct ggccgctgct gatgaaagcg gttttcgagg
     5461 agttggacag gcaggggtag gggacgggtg tggcattcat gtggcattca tctggcatcc
     5521 ctgcgggatg cgggggaggg gcggccggcg accggtggtg tcgctgcgct caaccaccgg
     5581 ctacgatcag gcatcctgcg ggatgcgggg gagagaccag cgaggacgcc ggtcccaccg
     5641 gcgggtgttc ctccggaatg tggatgggac tgaccagcga gggcgccggt cccaccggcg
     5701 gggggggtca gggctcgatg atcgtggctt cctggcgcat tttctcgtaa agctgaatca
     5761 cgggctcgcg gcgccgttcg tggctgaaat ggtcctcgac gaccggtcgc gcctcctcga
     5821 aggaaagctg cttgcgcggg cggcgcgccg ccacccacac caggtggtag cccatggggg
     5881 actggacgac ctcaccgacc tgattgacct gctgggtgaa ggcggcctcg accacttcgg
     5941 cgggcaggtt gacgtcggcg gtgatccagc cgatcttgcc gccctgggcc gcgtgggcgc
     6001 cgtcggagta gcgccgcgcg agctcggtga aatcctcgcc ggccagccag cggttgcgga
     6061 ggagcgccat ctcgctgcgc aagcgctcat cggcatccgc tgagtcgtcc ccctcggggc
     6121 ggaatttcac gatctgatag atcagcacgg cgtcgggttc gacataccat tcgcggtggg
     6181 cctggtagta ggcgcgcagt tcctcctcgg tcggcggctg gaggttgagc agtttgtccc
     6241 acagcttgat ctcggtcagt tggcggcgga tggccatgcg cagcgtgtgg gtggtgtcgc
     6301 ccatctcgcg catcgcgtcg gggagcgtgc ggccgtgggc ggtgaggtat tcggcgatgg
     6361 tgcggatctc gtcgtcgacc tcggcctcgg agacctcgat gccgtgttcg gcgacggcgg
     6421 cctcgatgat ccggtcgaag atgaggcggc gcagggcctc gtcgcggatc aggggggcct
     6481 tttcgcgcag ttcgcgttcc tcgatgcccg gcggggcctc ggccagcacg gcgtcgattt
     6541 cttcttccag ttgcaggcgc gtgatcggca ggccgttgac gatcatcacc gtttcgcgcg
     6601 ggtcctggcc cggctcgggc gcgggggggg tatagacgca cccgctcgcc gcgagcgccc
     6661 agacgacgat cagcaagcga tgacgaaggg agagagggga cacgggaggg cttccgggag
     6721 tgaaatcagg cagcggcacc aatcccagtc tatcacgacg ggcgggggag acaacagcgg
     6781 cgggggagcg tcgggcgagt cggggctcgg cggccgaggg gggcggaggc gcgatggcga
     6841 ttgcgatagc gatacggatc agcgattagc gccgctcctc atggttcacg aacacccaga
     6901 cgaccaacag gccgagcagg gcggtggcga gcgtgacgag gaggccgttg cggagcatca
     6961 agcgacggtg ggcggcgagc gcgtcgtcga tgggggcgag cggcagcgcg aggcgcagga
     7021 cgccgatcat ccggccgccg tgatgcagcg gcgtcgccac gtagagcatg cgggtcccca
     7081 gggtctcgct ggggcggacg gcatcgccgc gacgctccag gatcgcctgg cggatttccg
     7141 gccggtcgcc atggtttccc atgcgcgcgg gttcctcctc ggagtcggcg atgacggtgc
     7201 catcgggcag cagaagggtg atgcggacgc cggcatcggc gccgagacgc tgggcgaggg
     7261 cttcggcgtc ggcccagcgg ttctccacaa ggacgagcgg gaggaggggc gtcttgagga
     7321 tttgggccat cgccagcagg ctgtcggcct ggctgcgctc gtagaggcgg cgaatgggct
     7381 ggccggcgaa gaagatcgac ccgaccaggc agccgaccat caccaggacg aacaggccga
     7441 gcagcggcca acggaggcga tggcgtcggt gtggggtcaa gaccggcatc tccggcggag
     7501 gtggcgtccg ggggcgccac gacagcgcgc cccgcctcga ttggaaaacc cggctacgaa
     7561 ttcatgatag ccgagggggc gggctgaatc gaccttgatc cccggggggc gggcgatcac
     7621 gaggacgaag acgagggacg aagtgagggg tggggggaaa acgggagttg aaaggggcgg
     7681 agggagtggc gcagcatgtt gacttgattg ggtcccacga tctttggttt cgggattggt
     7741 ccctgacgtg acaatgcacg ccgatccgtc atcgctccgc ctgtttccgc cgtgtcatgg
     7801 cgtgctggcc gacttgcagc gctgcggctg gcggccggcg gaccaaccgt cggcgccgcg
     7861 gctggagttc cgcacgtatc agggggttca cgaggcgcag tgcgccgagg tggaaaatcc
     7921 cgccggccgg cggctgctgc tgatcggtct ggagtcggtg gtgctggcgc gggacgagga
     7981 cctgttcccg cgttttctga atctggcgat cgagacgcgg atggcgctgg cgtacggcgt
     8041 cgccgcgagc cgggccgatt ggctgctgct cttgacgcgg gaccggatcg atctctaccg
     8101 gctgccggag gagacggcgc tgcagcaggc gacggcggcg ggcgattacg atgaggggct
     8161 gctgccggaa ctggcggcgc tggcgcgcac ggaggtcgat gcgctgcggc tggggtcgca
     8221 gcatctgcgc ggggccgatt cgctgcgcgg ctggatccgg cactgggcgc tgcagctcgc
     8281 ctacgcggtt cagcttccgc ccgaccaggt ggagccggtg ctctggcgct ggattctgat
     8341 gctgcagtgg gtccggcgca ccgaacggag cgaagccaac ggcagctggg ggctgtattg
     8401 ccgggcggag ggtgaccgct gggccgtcgg ctacgacgcg cagacggcgt tcgccgacct
     8461 gaccaatgcg ctgagccggt tcgacgagct gttccattcc gagatgttcc gctgcgatct
     8521 ggagctgctg ctggggcggc tcaatcagat cgaggagacg acgctgctgg accggctgcg
     8581 cgccgaactg ctgatgcaca gccaggaccg gttcgaaccg gagaccgtgg cctggctgtt
     8641 caccgatctg gcgcgcgagc aggaggggtg gcggcgcgag gtgagcggcg tggagccgtt
     8701 gcgccggcgg ctgcaccatc aggggtggac gatataccgg ccgctggcgt gcgacgtgga
     8761 gcgccacggc ctgacgtttg cgctgcgtga gaccgagcgg ctggcgcact acctgaacaa
     8821 ccagatggtt tatgctcggc agcccgaatc gccctcgccg gcgacggcgc agcccgatct
     8881 gttcaagccg gagatccgcg gcgtgggccc gacgggccac ctggacgacc cggtcaatta
     8941 cctgttcgag gaggcgctgc ggctgcgggg ggtggaggcc gagagccggt tcggcgtggg
     9001 gctggtgttt ctgctcaagg cgctggcgct ggggcaggaa ttcgactggc cgttcttcgg
     9061 cgtcgacacg ctcgacgcgt tgttccgcga ggccggttga gcggctgact cgcgggtgcg
     9121 agggcggcag gaatttccgg acaccccttg cggggattcg gggcgttggg gttggcgacg
     9181 cagggttccg gcccgcggcc gtcaccccgc gcttgattcc ggccgatcgt tgcgcggact
     9241 tgggtatttg ccccctacgg gtcgccggtg caggagggag gcgaggggcg ggaagggacg
     9301 aggcgaggga cgagagacga gggacggttt ttcaagagag ccaaaacggg aagagaggca
     9361 agcggggggc gttgggcggg gtcagccggc gggttcgacg atctcgatca cgcgcgcggt
     9421 gcgattgagc cgggggttgt tgagtcttct gcgcagttcg ccgagcatct ggtggatcag
     9481 cgccgggtcg aacgacgccc gcagatgcgg gacaaactcc ggttcgcccg gcgcgccaaa
     9541 cacggccgcc tcccccgccg cgccgtcggg cgattccagc ccgtcctgga cggcgtcgca
     9601 cagggcctgg acgacgcgcg gctcgaactg aagtcccagg ttcgcctcga tctgttcgac
     9661 gatacgctcg atcgtcaggc ggggccgcca tggctgatcg ctggccatcg agtcaaacgc
     9721 ctcggccatc atcaggacgc gggcctcgat cgagagttcg tcgccgcgca ggcggtcggg
     9781 gtagccggtg ccgtcgagcc gttcgtggtg atggcggatg atctcggcga cgtcgcccca
     9841 ggggaagcgg aggtggctga tgagttcgta gccggcggcg gcgtgctcct cgacgcgcgc
     9901 gcgttcgccg ggggcgagcg ggccggactt gcgcaacagg tcgcggtcga tgatctgctt
     9961 gccgacgtcg tgcagcaggc cggccagata gagggcctcg cagcggtcgc ggtccagccc
    10021 gaggcggcgg ccgatctgcg ccgcgagggc ggcaacgcgc gcggaatggc cggggcgatg
    10081 gtcctgctcg gggccgtcga tgacgctggc gagggcgcgg accgtctcca gatagatgcg
    10141 gcgcagctcg cgattgagcc ggcgggtttc gtgaagttgc cggtcgagcc ggcggcgggc
    10201 gcgcgcatcg gtgatgcgca gggccatcac gccggccatc tcggtcagca cgtcggtttc
    10261 aagcggggac agcggccggc cgttgacgcg cggcccgagc aggagcaggc cggcgaactc
    10321 gtgcccgctg gtgaggggga cgatcagttg gatgttgcgg cggccgatga gttcgcggaa
    10381 gaggggctcg gcgtcggcga ggccgccggg cagcaggtgc gggtgaaacg gccggcggcc
    10441 ggcggccagc gcgcgggcca aggccggctt gagcgggagg ccctcggccg gggcgcggag
    10501 gcccttggcc gcggcggcgc gcagcagcgc ctcgtcctca tcccacagca ggatcattcc
    10561 ggcgccgacg gcgaacgtgc cgaggacgcg atagagcgat tcgcgcaggg gcttgttctg
    10621 gtcgagcttt ctcggcgtgc gcgccagcgc gccgcccagt tcgtggagcg aagcgagata
    10681 aaaggagagc aggcgggggt tgagcgattc gggcatggct cattccaagg ggaaggcgaa
    10741 gtggcgcgaa agttcggctc cgggttattg gccgagggcg cggcgctgcg tttcgcggtc
    10801 gtggcgcaga tgctcgcgcg cctggtcatc ggtcgggaag aacagcgcga cgctgcccag
    10861 gtcgaggacc tcgaagattt ccaggttggt tccgcccagg tgggtgaaat agacaaggcc
    10921 gccctgaaga tggacctttt cgataatgcc gacaaggatg gaaatgccga tggtattgat
    10981 cagctcgacc tgcttgaagt tgatcagaat gtatttgatg ccttccttga gcagggcatc
    11041 gcagaccttg tcgatccgtt cgccggtcag ggcgttgagg tatccggtcg gcacgatgac
    11101 gacatggtcg gcctcacgtt ggacggcaat ggttaactgg gtttcgggcg atgccacgtt
    11161 gggctcctcc atcctcggcg tctgcgacag cgggcgacag cgatcggcaa atgagctccg
    11221 ggggcggtcc cttcgccggg ggaacgcgcg ccggtgagtg aggcgaccgg ggccgccgcg
    11281 ctcaggcgtt ggttgattcg gcgcgccggc cgcgcggggc cgggcgccgt ttgacgagcc
    11341 gcagccgggt gcccccgggg aacggctcat aacccacctc gtccataaag cgctgcatca
    11401 ggcggatacc ccagccgcgc ttgttctcgg tctccagctt gcgccgggcg ttgggttcgg
    11461 ggatggcgag ggggtcgaac tcagtccctt cattctggat cacaacttcg agcgcgagcg
    11521 gatcgaccgc gaactggagt tcgaccattt tttcggggct cgggctgtgc tcgatcgcat
    11581 tgagcacgcc ttcgagcacg gcggtcttga cctggccggc ggcggcggga tcgaggccgg
    11641 cctggagcgc caccttttcg gccatcatcg cggcgatcac ttcgctctcg gaacgggccg
    11701 gcaggctgag ctggctgatt tcattttcgg gccgcgagcg cgagtggccg aggacgatca
    11761 cgccgccggg gcggccgggc ggagccgggg cgcttgccga ttcgccggcc gggtcggcgg
    11821 tctcgtccgg cgggaggtcg gccggcgtag ccgccgcggc ggacggatcg ccgtcgccgc
    11881 gccgcgcccg tttcagcagg tcgacgagca attcgatctg cgtcggaccg gaggcgagga
    11941 tctgctcctg gcgcaggcgt tccatcgcct cgggggaggc catcttgccc accagcaacc
    12001 agaggcggta gcgaccgggg cccatgcggc gctcgaaggc gcgggccgcg ccgagggcgc
    12061 ggtcgatctc cacgctggtc agcaccgaga cggcgcgcag gtcgaacagg agccagactt
    12121 cctcgtggct gcgctggtat ttgccgccgc gatagccgtg ggcgtagtag acagggggcg
    12181 cctggcgccg gtggcgctcg ctccagccgg cgggttgcca tggggcgacg ccgaggattt
    12241 cggggagcgg catcgcggcg gcggccgaag cggccagctc gcgcgcttcg gccagcgtca
    12301 tgcggccggc ggcctcgctg aagtcggcga actgaaagag catctccggc acgctctggc
    12361 cgcggaactc ggccagcagg gcgcgcgcgt cggggaagcg cgcggcggcc gggggggctt
    12421 cgggcgcggg gacctcggtc cgcagcaggg cgctcagcat gcggaggcgc tcggcgggga
    12481 gtgcgtcggt gtcggcgccg gcggtcaggt agaggtgcgc ccagtcggcc agcaggggaa
    12541 tcgccggcgc gccccagcgg gcgccgcggc gcgtcaacag gccgagccgg ccgatgccct
    12601 ccaggatgcg ctcggcttcg gcgggcgagc agggcagaaa cgccaggacc tcatcggccg
    12661 agagcgggcg atccaactga acggcgcgct ggatcaggcg cagggcgggc tcgcgcagat
    12721 tgagccggac gacatgctga aactgctgca tcagccggtt ggcccaatgg ctgccgcagg
    12781 ccagatccac ataggccaga tgcgcgcgac ggtggggatc gtcttcctcg gcgcccggct
    12841 cgacgctggc ggcccaggcg tggagccacg cggcgcgtcc gcccaggcgg cggacgacgg
    12901 gccggcaggc gggcagcggc agcgcccagt tggcccggac ggccgccgcg ctgatcaggg
    12961 cgagcgactc ctcctcgttg agcgggggga cggccagcca ctgaaaggcg tcggcagcgg
    13021 gccagtcctc gggcgattcg gggccctcga gcagcaggtc ggcgcccgca cggagggccg
    13081 cgcgcacaag gggcatgagc gcggcgcgcg cctcgggcca tggcaggggg gcaaggccat
    13141 cgacgatcag caggggcggg gccgagaggt cggcggcgag ggccgccagc acggcctccc
    13201 agcccaactc ggcatcatcg ggcgcggcgg tcagcgtcag ttcggccagg ctgtgcagct
    13261 cggcgccatg gcagatgccg gagaggtgcg acggatcgcg gcgcagccag acgcgctcgt
    13321 cggcctcgag ccggccctgg cggaacaggg tctggcggat gagcgcgcgg aggaggctga
    13381 gccagccgac gggggaggtg atcgccagcg cgccgggggt cgagccgcca ggggcttcgg
    13441 cttggaggaa atagaacggg atgggggttt cgcgcgcggc gaggtcgagg gtcaactggc
    13501 gcaggatctc gctgcggccg cagccggcgg gaccggtcag gccgagggca tcgggaccgc
    13561 ggccgtcggc gcggcgcgcc cagaggcgca ggcgcagcag cagatcgcgg cgtccggcga
    13621 attcctcgcg ggggcgcgcc gccgtcagat cccgccaggg gcgagtggaa tcgggcatcg
    13681 ccatcggccc catcggctcc tgtcccgagc gatccagggt ggccccgcca gcggtggacc
    13741 tggcgcgggc ggctcctcct ggagtggggc ggaccgttcg gcgcggtcgc ggaccggggt
    13801 agcctccgct gcccggcgtc gcggcgcgtc gttccggcgg ccgcccaccg agcctcatcc
    13861 tgagaggatt tccactatgc cgcatgagcc gcggagcagg ccagtaaatt cacgacaaac
    13921 cccctacgcg cgtcgcgccg acggcgcgcg gggaccgcgg cgcgccccgc ccgcgcgcgc
    13981 tcagccgacc tcggcatcct cgccatggcc gaagacctgg atcaccggct cgtgggtgca
    14041 ggccagcgcg cgggccagcg cggcttcggc ctggtggatc aactggtcgg cggagctggc
    14101 atcgacgccc cacagcgaga tgcccgcgcc gggccgaatg cccatttcct cggcgccgcc
    14161 gtgggcatcg aggacgtcct cgacgatcga gatgaggcgg aaggcgacgc ggcgcgcctc
    14221 gtgctcggag gtgtggggca acagcacggc ccagacggca tcgccgccat cgagccggaa
    14281 gcattcatcg taggggcgga gagcggcgcg gaactggtcg ctcagttcgg cctcggggag
    14341 cgcgacacgt tcggcttcga aagcgacgcc gaccaggacc agactgaagc cgtagccatg
    14401 gcgcgcgcag cggaccgatt cgacggccag gcggcgcgcc agaccgtcgc gtgggcaggc
    14461 gggcggggcg ggcgccggcg gggcggcgaa ggctggggcc ggcgtttcgc ggcggcgcgc
    14521 cggttccgga ttgcccggta cctgggcgag ccgggcggcg aggacctcgg ccagcacctg
    14581 catccagagg ggggcatcgc gctgccactc ggcggcggcg tcgcgcggct cgtgatcgat
    14641 ctcggccagc cagacgccca tggcctcgcc gcgggagaga gggaacggga gggcgacgcg
    14701 gcccgggccg cggaactcgc cggcgtagcg ccggacggtg tcgccctgct ggatcatgac
    14761 gcgccgctca ccgagggcca gcgcgccgat cgcgtgggcc ggcaggcggg tgtcgtgagc
    14821 gcgcggggag ggcgcctggg cggcgtcggc cggctcgcgc tcgaccgtcg agacaaggac
    14881 gggctcggca tcgaccgact cgcgcaggta gatgctcatc gcgtcgaacg ggaggaggcc
    14941 gcccagggcg cgcaggaatc gctcgcagat cgagccgggc tggtcggacg acgccagcag
    15001 ggtctgaagc tggaggacga actcgagcag gtgagtcagt tcggaggacg gccggaattc
    15061 gggctcgtcc ggctggcggg tcggcgccgc ggcgccggcc ggttccggcg gggggatttc
    15121 gagatccgtg gacggttcgt ggtccgtttc gcgcgcggca tcgcccgccg cccggcgcag
    15181 ctcgtggatc agcacgacgg gattcgaggg gaggtgggcg aagatcgttt cttcgtccag
    15241 gccctcggcc agcagacggt ccagcgcggc gtgcagcgac tgaagcgaga ggaagcggcg
    15301 gccgatccag cggctggtga ggcgcgggtc gggcgcgccg gccggcgcga ggcgctgcat
    15361 cgccatgccg aaggggcaga cgtggcggtc gggcggcgcg gcggggtcga tgccgcagcc
    15421 gcggttgcag acccgttccc cctcgcgcgt ccgggccagg aggcggcaga gcgggttttc
    15481 gttggccagg gcgatctgtt cgagcccgga gcggtgacgg agggtgaacg tcgtcagctc
    15541 ggtcaggccg accggaggat cgttgtcggt tggatcgggt tgagccgcgc ggaatgcgcc
    15601 ggaagccggc gtggaggccg gcgccttatc cagagtcaac atgagcaggc accacggcaa
    15661 cgggaacttg gtggcggcgt cggtcgtgag cggcgaccgg cgcctccctg ccagcgcagg
    15721 gttcagccaa cggaacagcg agtgcaacag cgaacgaatg acggatgaaa cgtcatccgc
    15781 gaaccccgac tgagcgggcc ggggttccgt gcccgaatcc atggttggac agaagggccg
    15841 gatctgtcaa ggccaatact tgcgatctga acaatcaggc gggaggggtc gaggacaagg
    15901 tgctcatgaa cctgtcattc acaacgaggg agaaaaatgg ctcgagagcc gaggacgagg
    15961 gacggggagc cgagagccga attctggaga tgggccttcg ggagcgagga agaggaggag
    16021 ggaggagaga cgaacttcag cggggggggg tggcgggagg gggggggggg ggcagggggg
    16081 gggggggggg gggggggccg ggctggcgct tgactttcgc cggggcgcgc ggcatcgttc
    16141 tttactttaa attcaaccgg gaacgcgtgg cgcgtggctc tcaatatcaa ggctctggtc
    16201 gaaggcgcat acacccataa cgcctcggac attcatgtgg tgcaggacag tccgacgttc
    16261 gtgcggatcg acggccacat gcggccggtg tccaacacgc acgtcacgaa ggaagacatc
    16321 gaggaactgc tgcggcagat catgccggcg gaggtgagcg gggacctgca gcagcggcgg
    16381 ggggcggatt ttgcgtggca gcccgatgaa cggatgcggt tccgcgtgtc ggcgtattac
    16441 gaacgcgacc ggctgcggct ggtgatgcgg ctgatccgga tccggatcgc cacgatcgac
    16501 gacctgggcc tgcccgaggt gctcaagacg atcgcgagct ggcatcgcgg cattacgctg
    16561 atgacggggg tgaccggctc ggggaaatcg acgacgctgg cggcgatgat cgactacatc
    16621 aacagccggg agagccgctg catcatcacg atcgaggacc cgatcgagat ggtgcacacg
    16681 aacaagaagt cgctgatcag ccagcgggaa gtcggccgcg acgtggagag cttccgccac
    16741 gggctggtgc aggcgctgcg ccaggacccc gacgtgattc tgatcggcga gatgcgcgac
    16801 ccggagacga tcagcacggc gctgcgggcc gccgaaacgg ggcactacgt cttttccacg
    16861 atgcacacgt ccaatgcgct gcacacggtc gaacgcatcc tgtcggagtt ttcggaggaa
    16921 gagcatgcgc tgctgcgcga ccaactggcc aacaacctga aggcgacgat cacgcagcgg
    16981 ctggtgcggc gcaagggggg cgtggggcgc gtcgccgcgc tggagatcat ggtcgtcaac
    17041 gacatgatcc gcaagctgct gctggagaat cagatcccct cgatcgcgac ggtgatccgg
    17101 gaacggcgcg acgggaacgt gctgttcgac cagttcctgg ccgatctggt gcgcagcgag
    17161 acgatcgacg aggccgaggc ctatcaatat gtggaagatg aggccgcctt ccgccgctac
    17221 gtgaagggcc ggctggcgac ggccgatcgg ggagggatca ttggctgagc acatcaccac
    17281 gacccccgcg cgcctgcgca ccggcgtggc cggactggac gagctgctgg gcggcggcct
    17341 gctggccggt cggacggtgc tgctgaaggg ttcgcccggt tcgggcaaga cgacgctcgg
    17401 gctgcagatg ctgatcgccg gcgccacgca gttcaatgaa ccggggatcc tggtcacgtt
    17461 cgagcaggcc cccgagcagc tttacgccga cacggccggg ttcggctggg atctgcgccg
    17521 gctgggcgag gaggggaagg tccagacggt cttcatcgag ccggaggaga tgatcgagca
    17581 ccgcgggcgg caggccaacc ggctgctggt caacatcgcc gactgggtgg acgactacgg
    17641 ctgccggcga atcctgatcg attcgatcag ccacctgagc acggtcttca ccaacgagga
    17701 agcgcgcgcg gggtttctga aattcgtcat gcagctcaag tcgatggggc tgacgccgat
    17761 catgacggcc gaactggacg cgctgcaggg gctgatggga ctggacgcgt acctggtcga
    17821 cgccgtgatc cacctgcatc acgagggggg gtcggtcggc acgcgcgagc ggcgcaatct
    17881 ggagatactg aagacgcgcg ggtatcagca cgtcggcggg cgccatccgc tggagatcgg
    17941 ccgcaacggg gtcgtcgtgt tcccccacac ctacccggcg gcggacgcgg ccgaggcggc
    18001 ggccgcggcg ccggtggggg cggacgcggc gccggtctcg acgggggtcc acgcgctgga
    18061 cctgctgctg tcggggggct accgggaggg ctcgacggcg ctgatcgccg gtctgtcggg
    18121 gacgttcaag tcgacgctgg cgggccattt tctgctcggc ggcggggggg aggcgccggc
    18181 gccggggctg tggctgagtt ttcatgagtc gacggccgac ctgatccagt cgttcggctg
    18241 gcgcgagatg gatctggccg gggccgtgga atcggggcgg gtgcgggtga tggaactggc
    18301 gccggggcag gaaccggtcg agaagatcta ctatcgcgcg gcggcgctga tcgccgagca
    18361 cgggatcggc cgcgtggtga tcgacacgct cggcgacctg acgctgggtc tggggcgggc
    18421 cgaggaggcg cgcgaggccg aacgctggtt cctgcgccgg ctgaaggcgc tgggggtgac
    18481 gacgctggcg acgcagcaac tggtcaaggt gaccgggcgc aacccgctgt cggagatcgg
    18541 cgcggcggaa ctggccgaca cgatcattta cctggggctg gtcgagatcg aaagccggct
    18601 ggagaaggtg atgtcggtgc tgaaacaccg ggggggcccg gtcagcggcg acctgcgcgc
    18661 gatcgcgtgc ggcgcggggg gcttgcaggt cagcgagcgg ttcgtggggc tgtcggggct
    18721 gctcgacggg acggcgcgcg ggcagcgcaa gacgcagatc gaagaggtct tccagccgct
    18781 gtacttcatc cgggattttc tgaagatggc ctcgcggccg gacctggacc ccgcgcgccg
    18841 cggccagatg ctggagcgtc tggaggggga aaccagccga gtgatcgcat cgctggcgca
    18901 gtattttgat cttcccctgg gcgaaaccga cacccgcaag gattcaaagg catgagcgct
    18961 cggatcctga tcatcgacga cgccgaccac ctgcgcgaag tgctgaagat gacgctggag
    19021 ttcagcgact acgaggtggc ggtggcgacc aatgggcgcg aggcgctcga gctgacccag
    19081 accagccggt tcgatctgat cttctgcgac atcgacatgc cggtgctcaa cggctatggg
    19141 ttcgtcgagg gataccgcgc ggcgcacggc gaccagacgc cgatcatcat gctgacggcg
    19201 gaggatcagg catcgatcaa caaggcgctg gcgctggggg cgaacgccgc gctggtcaag
    19261 ccgtttgagc cgtttcagtt gctggacgtg gtcaaggagc atgtgaagga aggctagggc
    19321 agcggagatg cggccccggg gagcaggggg gatggccgaa tggggcgggt ggcccagatt
    19381 gggaagaatt aactctgttt gtcttatttt tcttgcatcc aacgggcgga tgaaggaaga
    19441 tgcccctgtc agttgcgggc ggtgcgcaac cgtaaatatt gcggtgcggg gcgggccggg
    19501 gacagtaaga acgacggggg ggagcggccg gccggcgccg cgcggctgac gatagttgat
    19561 ccaactctca tttttgcagc gttccatggc caacatcgtt ccagtttcgg acttggcgcg
    19621 gggcttgcac agcaagcatg ccggaggtag tgaggatatg acgatccaga gcatttccac
    19681 ccggtccggc gagagcagtt cccccgaccc gcgcgcgttg cggctgacga ttctgcgcta
    19741 cgtgcgcgac catccgcgct cgttcgcctc gctgcagacg ctgatggaaa acctgcccga
    19801 actgggcgac ccgcgccgcg tgcagtcggc gctg
//
LOCUS       DAOPCO010000043        19662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2880_length_19662_cov_5.468761, whole genome shotgun sequence.
ACCESSION   DAOPCO010000043 DAOPCO010000000
VERSION     DAOPCO010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 19662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19662
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2880_length_19662_cov_5.468761"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="PLS90_05220"
     CDS             complement(<1..774)
                     /locus_tag="PLS90_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HPK01837.1"
                     /translation="MEQLELNGTWSVRMRGGKDALPARVPGCVHLDLLAAGRIPDPFF
                     RDNENDLQWIGEVDWIYTREFNAPAALLDHDRVLLVCEGLDTLATLELNGVRLGETNN
                     MFRTYEFDVKDALRPGANTLRIAFASTIPTPPDPNPLPADDPRARHRFKPFARRHLRK
                     EPCNYGWDWGPTLVTCGVWRPIRLVAFDVARLTDVAIRQRHASKSVRLEISVAIEPVR
                     AAALKARVTLSRDGRELARKITDVRRGAGAVSLIVRQPEL"
     gene            complement(788..2995)
                     /locus_tag="PLS90_05225"
     CDS             complement(788..2995)
                     /locus_tag="PLS90_05225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPK01838.1"
                     /translation="MTDLLALANPLQGTRSIHSFSRGNTLPFIGSPFAMTSWAMQTAD
                     GRWYFHPDHRQVRGVRATRQASPWMGDYGHFTLMPQVGERRLAPARRSSAYRPHEATF
                     QPHFLRFQLVRYETHVELTATPRAMIMRLVFLTGAREKRLLLETYPGQARIEIDPARR
                     AVRGYTRANSGGTPSNFASYFILKFDCELAADQCGLFREDEAFGGAEGTDDALGAIIG
                     LRPPAGGAVTVRVGTSFISLEQAERNLAGEIGDRALETVRDDVAADWRDLLDRFEIDA
                     DSDHDRRTFASCLYRAFLFPRRWHEPDAQGNPIHFSPHNGDVVPGVMYSDNGFWDTHR
                     TVYPFFSLVCPSRMQEMLEGWVNVYKESDWLPRWVNPGETGGMIGTHIDAVLADAVMK
                     GLRGFDVEAGYAGMVKQATREAPARYGRAGIEAWIRHGYLPADAARHSVSATLDYAFG
                     DFCIGQVAGALGKIEERDLMWRRAMNYRRLFDRATGFMRARDEHGRWIEPFDEFMWGG
                     PYVEGSAWQCSWAVPHDPAGLAELLGGDEALVARLDRMLALPPIYRADEYGEEIHEMT
                     EMACADFGQYAHNNQPVHHVLYLYAAAGRPDRTQSWVRRVLTELYGPGPDGFIGDEDN
                     GEMACWYLLSALGIYPLCVGRPEYVLGSPLFRRAVLRLEQGGELLVDAPANSPKNVYV
                     RRVAWNGAPHSRVVIDHRDLAQGGTLAFEMTGRPEWTEVAQADRPFSLSRLFP"
     gene            complement(3117..3500)
                     /locus_tag="PLS90_05230"
     CDS             complement(3117..3500)
                     /locus_tag="PLS90_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01839.1"
                     /translation="MSHRILIVDDDQDILASLSLFLGRQLGYQVETAAGGQEALDLAL
                     QHPFDLVILDVRMAGLSGPETWMRLKRLLPDCEVIFLTADEEFENTMDFMRFSLPSER
                     VLVKPLDDNSRLTRLIIGILGPPTP"
     gene            complement(3667..5688)
                     /locus_tag="PLS90_05235"
     CDS             complement(3667..5688)
                     /locus_tag="PLS90_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HPK01840.1"
                     /translation="MNFSRGFLQWAAAGLVALAILLRAVGLDQRPVIHDESMFAYYAW
                     SAMRNGHYTHLPILHGPTLILITGRVFSWFGDSIATGRAWIAAISLVVLAAGMGLWPR
                     RYRWWFAPVLFTSPILLYYSRFLRNEMLFSAVLMVGLLGAARGISRRQSAALWAMPGA
                     ACLIGLIAIKENALFVWATLASFGLVYAVRRLVWRGPARALRPRRRRRPRPAAPAGPP
                     ARLQRGTYMNPSYETVVKGRPGAVSDALSSGTETPRGDPAGVADAARESWLPTALAWI
                     WGAALGCGFVYFIYHVTTGPDSRHALANLASSWTYWREQHQLHRISGPIHYYLPILLL
                     YELPALLLLAAGLVWDAGLRRRRGLVYLGAVGLWLLVWLAWIPLARVGFIARVGEFLH
                     VEPNGSMLVLGVIIAVLLAWSILQLEQHRPLASFIGWWAACSLFQYSSAGEKVPWLAV
                     HIALPLIMMLGWVWAPRLRTMGRRGRALVLAWLVLTFAVALRNDYFLIFQGRDSDPRE
                     RIVYNHTSPRFDALCRQLLSNWEVYSDEIPLARRRVVLIGAPFWPGVWYFRDCNWVSP
                     AQAPEALGEGDDLIIGDQSRLEDLLRRVDMAEWNALRLSLRNAWVPAWPPGRPETDTW
                     AESLRAIPAWLGYLGRALPVWWKYYWDREPWTPTGEYPIIAIEPMTMRR"
     gene            complement(5730..8009)
                     /locus_tag="PLS90_05240"
     CDS             complement(5730..8009)
                     /locus_tag="PLS90_05240"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPK01841.1"
                     /translation="MLNELTLRAGALDFLWALYFLIGFELLSLPLRLALSGSPASPDA
                     RRILSRIAGPAALAMIVWFVGHLGGAWLDWPAAWLVFLLAAAVGGALSRGAGWAARLA
                     YDPNRLSRSLLLDALVAALFLGFMLVRRWAPEMTSEIGASAAEKFGNAMIFWANWFAP
                     AEPDGLPVTDYWFAGHALTYYDWGHFHWAWVGRMLGIPAPLALNLALARVTALVFEAA
                     WLLGRAAGLRAGWAAGAALLTAWGGNLAAIRGVRAVFGPTHAGINWTAYDFWAPSRAI
                     DHVVDEFPAFSAILGDFHAHHLALAWLLAWLALLLAGARWSGRGRATAWVFGATGVIL
                     AMAAVLANLWNLPLVAAVGVLALLLASRARRRTPTGLIAVLLALGCLLAAAIALQRGG
                     EPLPLATGGEPAGPLSRLPLRLLPAHLRSQPAQLLGLWGLPLLLVALLVFRGHLERRW
                     RGWKAALPALVCCVMAAALGNSLVFWLAAFVVVAGLAHPPRRLICRRAAVLLAGSCAV
                     LLGLEVVYWPDRFTGDLVRYNSYFKFSYPVWATLWIGAVACGARTVAGLRGAARGCYG
                     GLAGIVLAGALVYPVFAIPARIAGARRGDIRPHAATLDAYAFAANRPASAEEAELLAA
                     IRRLVPPGEVVIEAGSLKAYDYGGRVASLAGRPVPLGWIHHEQQWRGPAMFQELDARR
                     AEVDAFYQAPSPEEMRQRAEALGARWAIYGILERERYGPAAPARLQAAGRVARRVAAP
                     GGELFLFDFRPDPPPNVKP"
     gene            8493..9311
                     /locus_tag="PLS90_05245"
     CDS             8493..9311
                     /locus_tag="PLS90_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HPK01842.1"
                     /translation="MTNRIIPFRERLRQRRRLILFICTGNTCRSPMAAGYFRRLLEER
                     KIKDIEVRSAGVMTITGLLASQEAIQVMDGVSVDLRRHRSTQLTSELIRKADLILAMT
                     PFHRQTAVRMDENAKDKTYLLKEFTRSDLKNVQIADPMGCTLEVFKKCFKEIRSACDK
                     LIEHEFVTGRPAPPPPRKAEAARKAAARAKSAAGAGRSAKSASRKSASKATATKKTAG
                     TKPAAARGKAATAGQAAAKRPKTPAAKPAPAKTAPAKRTKAAGVASSASTRRNK"
     gene            9354..9803
                     /gene="rpiB"
                     /locus_tag="PLS90_05250"
     CDS             9354..9803
                     /gene="rpiB"
                     /locus_tag="PLS90_05250"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPK01843.1"
                     /translation="MRIAFANDHAAVAMRQPLLEELAALGHEVLDLGARDESRTDYPD
                     FARAVGDALHRGQADRGIVVCGTGVGICIAANKLRGIRCALVTDEYAAVMSRRHNDAN
                     VLALRGREFDPDLNRKLLRRWLETPFDGDERHQRRIDKIARLEELNE"
     gene            9955..11223
                     /gene="glyA"
                     /locus_tag="PLS90_05255"
     CDS             9955..11223
                     /gene="glyA"
                     /locus_tag="PLS90_05255"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPK01844.1"
                     /translation="MYDLIAAQDAQLAEAMRREYDRQRDKLELIASENFASPAVLEAA
                     GSVLTNKYAEGYPGKRYYGGCEHVDEVEQLAIDRAKELFGADHANVQPHSGSTANMIA
                     YFAVLEVGDVVMGMDLAHGGHLTHGLKVNFSGRFFNFVSYGVSPDTETIDYDALEKSA
                     KEVRPRLIVAGHSAYPRQLDFERLRAIADATDAMLMVDMAHFAGLVAADEHPSPVPYA
                     DIVTSTTHKTLRGPRSGFILCRAPYAKAIDKSAFPGAQGGPLMHIIAAKAVAFGEAAT
                     PGFKAYARQVRRNAAALGAALTAEGLRMVSGGTENHLLLVDLTPYGISGKQAETALDA
                     AAITCNKNMIPFDQRKPTEASGIRLGVPAVTTRGMNEEDMRRIGGWIARILRAPEDKQ
                     LQRQVSEEVHAFTAGFPLHVSVHEQVGAGV"
     gene            11256..11873
                     /locus_tag="PLS90_05260"
     CDS             11256..11873
                     /locus_tag="PLS90_05260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01845.1"
                     /translation="MPFSHYHQINQIVELIVELSPRRVLDVGVGFGMYGYLARLYLEL
                     SDGRQQYSDWQRVIDGIEAFPEYVTPVHEWIYNEIHRADALTVLPMLREPYDLVLAID
                     VLEHFPREQGVEFLRECTRCGRNVLISTPKEYWDQTDAFGNPYETHHHLWRKADFRSF
                     EDRLLIPNAYSLIVLIGPDAARVRRVLLKRKIRLWQRKLRHLVNR"
     gene            complement(12246..13526)
                     /locus_tag="PLS90_05265"
     CDS             complement(12246..13526)
                     /locus_tag="PLS90_05265"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK01846.1"
                     /translation="MTDPVAGYPAPPRAGYLEVLRGNRNYRLLWSGDLVSLLGDWFNL
                     IASATLIGMLTQSGAAVGGLLVVRWLAPFAVSPLAGVVADRFNRKHLLIAADLIRAVV
                     VLGFLFIRRPEQVWLLYVLTAVQLGISGFFFPARNAILPSLVTPRELGAANAISSATW
                     SSMLAFGTALGGLVAGTFGLHVAFLIDAATFLASAAFISRIRYHAEAEVQAHAPGPGA
                     VLRQYLAGLGFLRAHPGILVLALQKPLYFLTCNGGFQVIMVTLAERVYAIGHGGGISM
                     GLLFASVGVGTGLGPILARTITGDREPRMRVAVGSGYGVMALGLGLVASLGPLPVILI
                     GCLLQGFGGGMIWVFSTQLLLQKVPGPVRGRVFSTEFALLTLLGAMCAATTGWAVDRP
                     GWGLQPTIVVVALLPLLPATAWALWLLRPAAREI"
     gene            13829..14401
                     /locus_tag="PLS90_05270"
     CDS             13829..14401
                     /locus_tag="PLS90_05270"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HPK01847.1"
                     /translation="MHAMTAYPPILRLAPLALLALAMLTPMLPAAEIEYYMKMSDVDG
                     ESVDDGHKDWIVLLSFSHAINKRVEDFVTTGRSTFTPIVVTKIVDRSSPLLMRAIAGG
                     KLLPAVQIEAVNAETQQTYLRYEMKNVLISNWSTSGNADIQPTEQLSLNFEEIKVIYH
                     RYTFGSPDPVESVEMGWSVIDDAEAPRKTE"
     gene            14503..15108
                     /locus_tag="PLS90_05275"
     CDS             14503..15108
                     /locus_tag="PLS90_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HPK01848.1"
                     /translation="MQHAATCNGRLILWGLGLLFLPFAPVFAQETSRGFLSLPGVEGE
                     STDEFHKDWIDVLSVDHAIAKLVEDFATTGRSRHAPILVRKRIDKSSPRLMQACANGT
                     TLSEAVIDLVKAESGLRFLRIRLTDVLVSSYSVGVAKEDALGLPREEITLVYHKIQVT
                     YFRYSSGSNDPVEEVEMQWDAVNDVEDTGKTATEEETLRLE"
     gene            complement(15136..17307)
                     /locus_tag="PLS90_05280"
     CDS             complement(15136..17307)
                     /locus_tag="PLS90_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK01849.1"
                     /translation="MACRSLLWTARRLFPLLTCCFALACATPGTPDYALLRPEAPLEA
                     GAARGGVPSQSLNGAWRFKLQPPGGVELADAQFYLPQYDASGWREIQVPSHWELQGYE
                     EPGRREPSRLSDVAASAGLYRREFTPPAAWRGRRIFLEFEGALWGCQAWVNGRLVGQD
                     SSAYTPKRFDITQHVTPGAANLLAVRVTKLSPEMPFDADAAWALSGLFRDVRILARPE
                     FHLTEPAVTTELTRGGTRALVTVEAGARRYAAAGAMAGCDILCELYDPAGARVASGTV
                     DLAAASVEAPSSFERGGRAQLIVDRPRLWTAETPHLYRLELKLMHGGSLLDTQTHWVG
                     LRQVRIVNQALQVNGRKVTLRGIDWHGLHPETGRALTEEQLRHDVRLLKLANANLVRI
                     IDHPPDPEFLALCDRFGLYVICPMPVGGMAHSLVVEPYAAELRSRVEATIRMARLHPS
                     VILWSPGGEPAWTPLVEATARLVRELDPSRPLLVALAVPSDREADLEFLQGPPLADVL
                     ALPYPVTEALALLADECPMPVFFLRQPNEPAPDVEERLERWRTIEARADLAGGTAGTL
                     ADLDLKQLVVPLSPDAPLPRTAATGGVARPEGLMAADRQPKDAYWATRVVYSPIHVHE
                     SELITRPGRRRLTVTLSNHYDFLNLNRLRMRYGIERDGRALERETKIVTLAPHEHGAV
                     RMRVDVPELTESVATRLMIQFVDAEDQVLYSRGVRLRDARW"
     gene            complement(17443..17889)
                     /locus_tag="PLS90_05285"
     CDS             complement(17443..17889)
                     /locus_tag="PLS90_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01850.1"
                     /translation="MRVRIRLVCRVAALLLLAGCAHHPTVDQDSTEPAGAVLSEAVGS
                     EPAGEPSAVWHVGRAGRLALDPALYLADVALALPQFLRPLRAVGTVVLAPFKVFFPPG
                     FVLDLATAPADLAAPFVALYHIVTGLPRLIWSPLLALWHLGAAAAG"
     gene            complement(17941..18894)
                     /locus_tag="PLS90_05290"
     CDS             complement(17941..18894)
                     /locus_tag="PLS90_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HPK01851.1"
                     /translation="MAKLLEYNATLTDRVDLTPKLSILRVKPDPSWGATAGQIPDFEA
                     GQYVVLGMNNEREPEKGSVQRPYSIASPPEQKEFLEFYIRYVDQPESDNPLTHLLWPL
                     KVGDRLYLGKKITGHFTLAKTLDPNDPRLKVFVAAGTGLAPFVSMILSYVTRGVSPGQ
                     FAVLHGASIPQDLGYRDDLRRLFASLPERYMPTISRPAMAPEWEGDVGRVETFFDDEK
                     LEVLEERLGLKPGELEPGRCVVYICGLYGTIHNTLVRLMKRGFVPNDRLIRKELGLLD
                     LEPTLFFEQYDNEPVLDVKNREAMEQLLAETPFAGRLVSNT"
     gene            complement(19037..>19662)
                     /locus_tag="PLS90_05295"
     CDS             complement(19037..>19662)
                     /locus_tag="PLS90_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPK01852.1"
                     /translation="GLIVSGGHTSLVWVGSPARAETIAQTRDDAVGEAYDKVARVAGL
                     GYPGGPAIDRLAATGAPDGIHLAPPMMRRDKPDFSFSGLKTAAGRELERLRAEHGGEL
                     PAGTLANFCAAFQAAAVEALLGKALDAVAARRAADLVITGGVACNRGLRRRAAELSAH
                     RPGLRIWFPHPLLCTDNAAMIAGLAWHLEPLAPRAALTLNADPGLGI"
BASE COUNT         3488 a         6778 c         6598 g         2798 t
ORIGIN      
        1 cagttcgggc tggcggacga tcagcgagac ggccccggcg ccgcggcgga cgtcggtgat
       61 tttccgggcg agctcccggc cgtcgcgcga aagcgtgacg cgcgccttga gcgccgccgc
      121 gcgcaccggc tcgattgcca ccgagatttc cagccttacc gacttgctgg cgtgccgctg
      181 gcggatggcg acgtcggtca ggcgagcgac gtcgaacgcg acgagccgga tcggccgcca
      241 cacgccgcac gtcaccaggg tcggccccca gtcccacccg taattgcacg gctccttgcg
      301 caggtgacgc cgcgcaaagg gtttgaaacg gtgccgcgcg cgcgggtcgt cggccggcaa
      361 cgggttggga tcggggggcg tcgggatcgt cgaggcgaag gcaatgcgca gcgtgttcgc
      421 gccgggccgc agcgcatcct tcacgtcgaa ttcatacgtg cggaacatgt tgttggtttc
      481 accgagccgc acgccgttga gctccagcgt cgccagcgtg tcgagcccct cgcagaccag
      541 caggacgcgg tcgtggtcga ggagcgcggc cggcgcgttg aactcccgcg tgtaaatcca
      601 atcgacctcg ccgatccatt gcaggtcgtt ttcattgtcg cggaagaagg gatcggggat
      661 cctgccggcc gccagcagat ccagatgcac gcagcccggc acgcgggccg ggagggcatc
      721 cttgccgccg cgcatgcgca cggaccatgt cccgttgagc tcgagctgtt ccatcgtata
      781 ccgttcctta cggaaataat cgcgagagcg agaacggccg atccgcttgc gccacctccg
      841 tccattccgg ccggccggtc atctcaaagg ccagcgtccc gccctgggcc aggtcgcggt
      901 ggtcgatcac cacgcgcgaa tgcggcgcgc cgttccacgc gacgcgacgg acgtacacgt
      961 ttttcggcga attcgccggc gcatcgacaa gcagctcccc gccctgctcc agccgcaaga
     1021 ccgcccgccg gaatagcgga ctgcccagga cgtactccgg ccggccgacg cacaacggat
     1081 aaattccgag cgcgctgagg aggtaccagc aggccatctc gccgttgtcc tcgtcgccga
     1141 tgaagccgtc gggccccggg ccgtaaagtt cggtcaggac gcgccggacc caggattgcg
     1201 tgcgatcggg ccggccggcc gccgcgtaga gatagagcac gtgatggacc ggttgattat
     1261 tatgggcgta ttggccgaaa tcggcgcagg ccatttcggt catttcgtgg atttcctcgc
     1321 catattcgtc ggcacggtag atcggcggca gcgccagcat ccggtcgagc ctggcgacca
     1381 gcgcttcgtc gccgccgagc agttcggcca gcccggccgg gtcgtgcggg acggcccagc
     1441 tgcactgcca ggcgctccct tcgacatagg gaccgcccca catgaactcg tcgaacggct
     1501 cgatccagcg gccgtgctcg tcgcgcgcgc gcatgaagcc ggtggcgcgg tcgaacagac
     1561 ggcgatagtt catcgcccgg cgccacatca gatcgcgctc ctcgatcttg cccagcgcgc
     1621 ccgccacctg gccgatgcag aagtcgccga aggcataatc cagcgtggcc gatacgctgt
     1681 ggcgcgcggc gtccgcgggg aggtagccgt ggcggatcca ggcctcgatg cccgcccgtc
     1741 cgtaccgggc cggcgcctca cgcgtggcct gcttgaccat ccccgcgtag ccggcctcga
     1801 catcgaaccc gcgcaggccc ttcatgacgg cgtcggccag aacggcgtcg atgtgcgtgc
     1861 cgatcatgcc gccggtctcg ccggggttga cccagcgcgg cagccagtcc gattccttgt
     1921 aaacgttgac ccagccctcc agcatctcct gcatccgcga cgggcagacg agcgagaaga
     1981 acggatagac cgtgcggtga gtgtcccaga acccattgtc ggaatacatc acccccggca
     2041 cgacgtcgcc gttgtgcggg ctgaagtgga tcgggttgcc ctgggcgtcg ggctcgtgcc
     2101 accggcgggg gaacaggaag gcgcggtaga ggcaactggc gaacgtgcgc cggtcgtggt
     2161 cggaatcggc gtcgatctcg aaccggtcga gcaggtcgcg ccagtccgcc gccacgtcgt
     2221 cgcgcacggt ttcgagcgca cggtcgccga tttcgcccgc gagattgcgc tcggcctgct
     2281 ccaggctgat gaatgacgtg cccacgcgga ccgtgacggc gccgccggcc ggcggccgca
     2341 gcccgatgat cgcgcccagc gcatcgtcgg ttccctcggc gccgccgaag gcttcatcct
     2401 cgcggaacag gccgcactga tccgccgcca gttcgcagtc gaatttcaaa atgaaataac
     2461 tcgcgaaatt ggacggcgtg ccgccgctgt tggcgcgtgt gtagccgcgc acggcgcgcc
     2521 gcgccgggtc gatctcgatc cgcgcctggc cggggtaggt ctccagcagc aggcgcttct
     2581 cgcgggcccc ggtcagaaag accagccgca tgatcatcgc gcgcggcgtg gcggtgagtt
     2641 ccacgtgggt ctcgtagcgg acgagctgga agcgcaggaa gtggggctga aacgtcgcct
     2701 cgtgcgggcg gtaggccgag gagcggcgcg ccggcgcgag gcgccgctcg ccgacctgcg
     2761 gcatcagcgt gaagtggccg taatcgccca tccacgggct ggcctggcgc gtcgcgcgga
     2821 ccccccgcac ttggcggtga tcggggtgga aataccagcg gccgtcggcg gtctgcatcg
     2881 cccaggacgt catcgcgaac ggcgagccga tgaacggcag cgtattgccc cgcgagaagg
     2941 aatgaatcga gcgggtgccc tggagcgggt tggcgagcgc gagcaaatcg gtcattggag
     3001 catcccgcgt ggaaaatcgg cgggcgagga cgcctcccac taaagccgcc ccaaaggtat
     3061 cgattcgcgc gggcgctgtc gatagccgcg tgcgggacgc atgcgttccg cgggcgctat
     3121 ggcgtcggcg ggccgaggat tccgatgatc aggcgggtca ggcgcgagtt gtcgtccagc
     3181 ggcttgacga ggacgcgctc gctggggagc gagaagcgca tgaaatccat cgtgttctcg
     3241 aattcctcgt ccgcggtcag aaaaatcact tcgcagtcgg gcagcagccg cttcagccgc
     3301 atccatgttt ccggcccgga gagtcccgcc atgcgcacat ccaggatgac cagatcgaac
     3361 ggatgctgaa gggcgagatc cagggcctcc tggccgccgg cggcagtctc cacctggtaa
     3421 cccagttggc ggcccaggaa caacgagagc gaggccagga tatcctggtc gtcatcgacg
     3481 atcagaatgc ggtgggacat gaacgggccg ctcccatcag gatgcccggc gccccagcgg
     3541 agggctccgg accacaacgg cgcgcagcca gcgacaaccc cgtgacttcc gctggattag
     3601 aacataccca atccgatgaa gccagaacca atgccggcgg gcgatgcggc cgcgcgccgg
     3661 ccgccgtcaa cggcgcatcg tcatcggctc gatcgcgatg atcggatact cgccggtggg
     3721 cgtccacggt tcacggtccc aatagtattt ccaccagacc ggcagggcgc ggccgagata
     3781 gccgagccac gccgggatgg cgcggagcga ctcggcccag gtgtccgtct ccggtcggcc
     3841 cggcggccag gcgggcaccc aggcgttgcg cagcgacagc cgcagcgcgt tccattcggc
     3901 catgtcgacg cgccgcagca gatcttccag gcgggactgg tcgccgatga tcagatcgtc
     3961 gccctcgccg agcgcttcgg gcgcctgcgc gggcgagacc cagttgcagt cgcggaaata
     4021 ccagacgccg ggccagaacg gcgcgccgat caggacgaca cgccgccgcg ccagcgggat
     4081 ttcgtcgctg tagacctccc agttggaaag caattggcgg cacagcgcgt cgaaccgggg
     4141 cgaggtgtgg ttgtagacga tccgctcgcg ggggtcggaa tcgcgaccct ggaagatgag
     4201 gaaatagtcg ttgcgcagcg ccaccgcgaa cgtcagcacc agccaggcga gcacgagcgc
     4261 ccggccgcgg cgccccatcg tccgcaggcg cggcgcccag acccagccca gcatcatgat
     4321 cagcggcagc gcgatgtgca ccgccagcca cgggaccttt tcgcccgccg aggaatactg
     4381 gaacagcgaa caggcggccc accagccgat gaacgaggcc agcgggcgat gctgctcaag
     4441 ctgcaggatc gaccacgcca gcagcacggc gatgatcacg ccgagcacga gcatcgagcc
     4501 gttgggctcg acgtggagga actcgccgac gcgcgcgatg aagcccaccc tggcaagcgg
     4561 gatccacgcc agccagacga gcagccagag cccgacggcg ccgaggtaaa caagtccgcg
     4621 ccggcggcgc aggccggcgt cccagacgag ccccgccgcg agcagcagca gcgcgggcaa
     4681 ttcgtagagc aacaggatcg gcaggtaata atgaatcggc ccgctgatgc ggtggagctg
     4741 atgctgctcg cgccagtagg tccaactgga ggcgaggttg gccagggcat ggcggctgtc
     4801 ggggccggtg gtgacgtgat agatgaagta gacgaagccg cagccgagcg cggcccccca
     4861 gatccaagcc agcgccgtcg gcagccagct ttcgcgggcg gcgtcggcca cgccggccgg
     4921 gtcgccccgc ggggtttcgg tcccggacga caacgcatcg gacacggcgc ccggccggcc
     4981 cttcaccacc gtttcgtagc tcgggttcat gtaggtgccg cgctgcaagc gggccggcgg
     5041 gccggcgggg gcggccggac gcgggcggcg gcggcggcgc ggacgcaagg cgcgcgccgg
     5101 gccgcgccag accagccgcc gcacggcgta gaccagaccg aacgaggcga gcgtcgccca
     5161 gacgaacaac gcgttttcct tgatcgcaat caggccgatc aggcaggcgg cgcccggcat
     5221 cgcccacagc gccgccgact gacgccgcga gatgccccgc gcggcgccga gcagaccgac
     5281 catcaggacc gcgctgaaca gcatctcgtt gcgcaggaag cggctgtaat acagcaggat
     5341 cggcgaggtg aacagcaccg gtgcgaacca ccagcggtag cgcctcggcc acaggcccat
     5401 ccccgcggcc aggacgacca gcgagatggc tgcgatccag gcccggcccg tcgcgatcga
     5461 atcgccgaac caggaaaaca cccggcccgt gatgaggatc aacgtcgggc cgtgcaggat
     5521 cggcagatgg gtgtaatggc cgttgcgcat cgccgaccag gcatagtagg cgaacatgct
     5581 ttcgtcgtgg atgaccggcc gctggtcgag gccgacggcg cgcaacagga tcgccagcgc
     5641 gaccagtccg gcggcggccc actggaggaa cccgcggctg aaattcatcc cggctccgcg
     5701 aaggagcgcg ctggcgggag gcggttgaat catggtttca cattgggagg ggggtccggg
     5761 cgaaaatcaa ataaaaaaag ctcgccgccg ggggcggcca cgcgccgcgc cacgcgcccg
     5821 gccgcctgca ggcgcgccgg cgcggcgggg ccgtagcgct cccgctcgag gatcccgtag
     5881 atcgcccagc gggcgccgag cgcctcggcg cgctggcgca tctcctccgg cgagggggct
     5941 tgatagaagg cgtcgacctc cgcgcggcgc gcgtccagtt cctggaacat cgccggcccg
     6001 cgccattgct gctcgtgatg gatccaaccc agcggcacgg ggcgacccgc caggctcgcc
     6061 acccgcccgc cgtaatcgta ggccttgagc gagccggcct cgatcaccac ctcgcccggc
     6121 ggaaccagcc gccggatcgc cgcgagcagc tcggcttcct cggccgaggc cggccggttg
     6181 gccgcgaagg cgtaggcatc cagcgtcgct gcgtgcggcc ggatgtcgcc ccggcgagcc
     6241 ccggcgatcc gcgccgggat ggcgaaaacg ggatagacca gtgcccccgc caggacgatc
     6301 cccgccaggc cgccgtagca cccgcgcgcg gcgccgcgca ggcccgcgac cgtccgggcg
     6361 ccacaggcga cggcgccgat ccacagcgtc gcccacaccg gatacgagaa tttgaagtag
     6421 gagttatacc ggacgaggtc gccggtgaac cgatccggcc aatagaccac ttccagtccg
     6481 agcagcaccg cgcacgagcc cgccagcagc accgccgcgc gccggcaaat cagccggcgg
     6541 ggggggtgag ccaggccggc gacgacgacg aacgcggcca gccagaagac cagcgaattg
     6601 cccagcgcgg cggccatcac gcagcaaacc agggccggca gcgccgcctt ccagccgcgc
     6661 cagcgccgct ccagatgccc gcggaagacg agcagcgcga ccagcagcag cggcagcccc
     6721 cacaagccga ggagctgggc gggctgcgat cgcaggtgcg cgggcagcag ccgcagcggc
     6781 aggcggctga gcggccccgc cggctcccct cccgtcgcca gcgggagcgg ctccccgccg
     6841 cgctgcaggg cgatggccgc cgccagcagg caacccaggg cgagcaagac ggcgatcaga
     6901 ccggtcggcg tgcggcggcg cgcgcggctt gccagcagga gggccaggac gccgacggcg
     6961 gccacgagcg gcagattcca gagattcgcc agcacggccg ccatcgcgag gatgacgccg
     7021 gtcgcgccga agacccacgc cgtggcgcgc ccgcggcccg accagcgcgc tccggccagc
     7081 aacagggcga gccacgccag cagccacgcc agcgcgaggt gatgcgcatg gaaatcgccc
     7141 agaatcgctg aaaacgcggg gaactcgtcg acgacatggt cgatcgcccg cgagggcgcc
     7201 cagaaatcat aggccgtcca gttgatgccc gcatgggtcg gcccgaagac cgcccgcacg
     7261 ccgcgaatcg cggccaggtt gccgccccat gccgtcagga gggcggcacc cgcggcccag
     7321 ccggcgcgca gcccggccgc ccggccaagc agccacgccg cctcgaagac cagggcggtg
     7381 acgcgggcca gcgccaggtt gagcgcgagc ggcgccggga tgcccagcat ccgccccacc
     7441 caggcccaat gaaaatggcc ccagtcgtag taggtcagcg catgcccggc gaaccagtag
     7501 tccgtcaccg gcaggccgtc gggttcggcg ggggcaaacc agttggccca gaagatcatg
     7561 gcattgccga acttttcggc ggccgaggcg ccgatttcgg acgtcatctc gggcgcccag
     7621 cggcgaacca gcatgaaccc caggaacagc gccgcgacga gcgcatcgag caggagcgat
     7681 cggctcaacc ggttcgggtc ataagccagc cgagcggccc agcccgcgcc gcgcgacagc
     7741 gccccgccga cggccgccgc cagaaggaag accagccacg ccgccggcca gtcgagccac
     7801 gcgccgccca gatgaccgac gaaccagacg atcatcgcca gggcggcggg cccggcgatc
     7861 cgggaaagaa tgcggcgggc atcgggcgac gccggcgagc cggacagcgc cagccgcagc
     7921 ggcaggctga gcagctcaaa gccgatcaga aaatagagtg cccagagaaa atccaacgcg
     7981 cccgccctga gtgtgagttc gttgagcatg gaacggagac gtcaggatac agcttctggc
     8041 agtcgtctct cgacgctcgt cctcgtgatc gattccggtg gttctgccgt ccccggcggg
     8101 acgctgcctt tttgtgtttc accgttttcc cgccacggac gtagcgggct gaagtctgcc
     8161 gtccccgagg ggatggtctt ctttcagtcc ggcaagttcc ccgggaggat ttgaatatta
     8221 ttcgtttata tcttagtatc ctttcattat tttatatcct tagtatactc tgccttccct
     8281 gccttcccgt aaaaaatgac gctatctgac gaatcttcgg tgaaatagtc gcacgggccc
     8341 tcttgaaaat caggccaaaa tctctataat gtattgcgcc gtgcagaact cgccgacgac
     8401 cagggctggg tggcacggcg ggtctccctc tccggcgttc tgtccgcgga cgaagtaaac
     8461 gacctatctc cacccctgta aaggcttgga gcgtgacaaa ccgcatcatc ccctttcgtg
     8521 agcgtcttcg tcaacgtcgc cgcctgatcc tgttcatctg cactgggaat acgtgccggt
     8581 ccccgatggc cgccggttat tttcgccgcc tgctggaaga acgcaagatc aaggatatcg
     8641 aggtgcggtc ggccggcgtg atgaccatca ccggcctgct ggccagccag gaggcgattc
     8701 aggtgatgga cggcgtctcg gtcgacctgc ggcggcaccg ttccacgcaa ctgacctccg
     8761 agctgatccg caaggccgac ctgattctgg cgatgacgcc cttccaccgc cagacggccg
     8821 tgcggatgga cgagaacgcc aaggacaaga cctatctgct caaggaattc acgcgcagcg
     8881 acctgaagaa tgttcagatc gccgatccga tgggctgcac gctggaagtc ttcaagaagt
     8941 gtttcaagga gatccgctcg gcgtgcgaca agctgatcga gcatgaattc gtaaccggac
     9001 gcccggcgcc gccgcccccc cgcaaagcgg aggcggcgcg caaggccgcc gcccgggcca
     9061 agtccgccgc cggcgccggc cggtcggcca agtcggcgag ccgcaagagc gccagcaagg
     9121 cgacggcgac gaagaaaacc gcggggacca aacccgcggc cgcgcggggc aaggcggcca
     9181 ccgccggtca agccgcggcc aagcgcccca agacgccggc ggccaagccc gcgccggcca
     9241 aaaccgcgcc cgccaagcgc acgaaggcgg ccggggtcgc cagctcggcc agcacgcggc
     9301 ggaacaaata gcacaccggc tggatatcgc gaaaggaccc tgccccaccc cccatgcgga
     9361 tcgcgttcgc gaacgatcat gcggccgtcg ccatgcgcca gccgttgctc gaggagctgg
     9421 ccgcgctggg ccacgaggtg ctcgacctgg gcgcccgcga tgaatcccgg accgattacc
     9481 ccgactttgc gcgggcggtc ggcgatgcgc tgcaccgcgg ccaggccgat cgcggcatcg
     9541 tcgtctgcgg caccggcgtc ggcatctgca tcgcggccaa caagctgcgc gggattcgct
     9601 gcgcgctggt gaccgatgag tatgccgccg tcatgtcgcg ccggcacaac gacgccaacg
     9661 tcctggcgct gcgcggacgc gaattcgatc cggacctcaa tcgcaagctc ctgcgccgct
     9721 ggctggagac cccgttcgac ggcgatgagc ggcatcagcg acggatcgac aagatcgccc
     9781 ggctcgagga gctgaacgag tagcctcccc cggcgttccg ctcgcgcgcc ggccccgtgg
     9841 cggcgatcac cggcccgtcc atccgtcccc ccgcgatgtc cccctcgcgc cggcccgccg
     9901 aggggccgcc ggcgccgccg gaatcttcat caacccgttc aggagcctgc gttcatgtac
     9961 gacctcatcg ccgcccagga tgcccaactg gccgaggcca tgcgccgtga atacgaccgt
    10021 cagcgcgaca agctcgaact gatcgcgtcg gagaacttcg cctcgcccgc cgtgctggaa
    10081 gcggccgggt cggtgctgac caacaaatat gccgagggct atccgggcaa acgctactac
    10141 ggcggctgcg agcatgtcga cgaggtcgag cagttggcca tcgaccgggc gaaggagctg
    10201 ttcggcgccg accacgccaa cgtgcagccg cactcgggca gcacggccaa catgatcgcc
    10261 tacttcgccg tgctcgaggt gggcgacgtc gtcatgggga tggacctggc gcatgggggc
    10321 catctgacgc acggcctcaa ggtcaacttt tcgggacggt tctttaattt cgtcagctac
    10381 ggggtcagcc ctgacaccga gacgatcgac tacgacgcgc tggagaaatc agccaaggag
    10441 gtgcggccgc ggctgatcgt cgccggccac agtgcctatc cgcgccagct ggatttcgag
    10501 cggctgcgcg cgatcgccga cgccaccgac gccatgctga tggtggacat ggcgcacttt
    10561 gccgggctgg tcgcggccga cgaacacccc agtcccgttc cctatgcgga tatcgtcacg
    10621 tcgacgacgc acaagacgct gcgcggcccg cgcagcggct tcatcctctg ccgcgcgcca
    10681 tacgccaagg cgatcgacaa gtcggcgttc ccgggggcgc agggcgggcc gctgatgcac
    10741 atcatcgcgg ccaaggcggt cgcgttcggc gaggcggcga cgcccggctt caaagcctat
    10801 gcgcggcagg tgcgccgcaa tgccgcggcg ctcggcgcgg cgctgaccgc cgagggcctg
    10861 cgcatggtca gcggcggcac cgagaaccac ctgctgctgg tcgacctgac cccctacggc
    10921 atcagcggca agcaggccga gaccgcgctc gacgccgccg cgatcacgtg caacaagaac
    10981 atgatcccgt ttgaccagcg caagccgacc gaggccagcg ggatccgcct gggcgtgccc
    11041 gccgtgacca cgcgcgggat gaacgaggaa gacatgcggc gcatcggcgg ctggatcgcc
    11101 cggatcctgc gcgcgcccga ggacaagcag ttgcagcgcc aggtgagcga ggaggtgcac
    11161 gcgttcaccg cggggttccc gctccatgta tcggttcatg agcaggtcgg cgccggcgtc
    11221 tgaatcccgg gcaaaggcag cgcgtcgcga ccaccatgcc gttcagccat taccaccaga
    11281 tcaaccagat cgtcgagctg atcgtggaac tgtcgccccg ccgcgtgctc gatgtcggcg
    11341 tcgggttcgg gatgtacggc tacctggcgc ggctctacct tgagctctcg gacgggcgcc
    11401 agcaatacag cgactggcag cgcgtcatcg acggtatcga agccttcccc gaatatgtca
    11461 cgccggttca cgagtggatc tataacgaaa tccatcgcgc ggacgccctg accgttctgc
    11521 ccatgctccg cgagccctac gacctggtcc tggcgatcga tgtgctggag cacttccccc
    11581 gcgagcaagg ggtcgaattc ctgcgcgagt gcacgcggtg cggccggaac gtgctgatct
    11641 cgacgcccaa ggagtactgg gatcagaccg atgccttcgg caacccgtac gagacccacc
    11701 atcacctctg gcgcaaagcc gacttcagga gttttgagga tcgcctgctg attccgaatg
    11761 cttactcgct gatcgtgctg atcggccccg acgcggcgcg ggtgaggcgc gtgctgctca
    11821 agcggaagat ccggctgtgg cagcgcaagc tgcggcatct ggtgaatcga tagcggatcg
    11881 gggtgaactt gccccggcgg gcggcccgct ccagcccaga taccgcgcct cgtcaaccat
    11941 cgccgcgaag attctgaaat gctcctcgat atcgatcggc cgggaacgaa gatactcatt
    12001 ctcgaatgcc tccagaagaa gcgggttcct gatcatcgcg gctgcaccgg cctccatggc
    12061 tgatttcagg aaagagcaac cgatggcggg gctcaagcgg gcgcttcggc cggtgcgggg
    12121 cgctccggat aaggcgcgct cccggctccc gcacccaggt catggttccc gaaataacgt
    12181 ccgcggtgca ttgaacatga ggtgagagac tccaccgcga caaccaatag ctgtccgagg
    12241 cgtacttaaa tttctcgcgc cgccggcctc aggagccata gcgcccatgc cgtcgccggc
    12301 agcaagggca gcagcgccac cacgacgatc gtcggttgca gcccccaccc cggccggtcc
    12361 acggcccaac ccgttgtggc ggcgcacatc gcccccagca gggtgagcag cgcgaattcg
    12421 gtcgagaaga ccctcccccg caccgggccc ggcactttct gcaacagcaa ttgcgtgctg
    12481 aagacccaga tcatcccgcc gccaaagccc tgcagcaggc agccgatgag gatcaccggc
    12541 agcggcccga ggctcgccac caagcccagc ccgagcgcca tcacgccgta gcccgagccg
    12601 accgccaccc gcatgcgcgg ctcccgatca ccggtgatgg tgcgcgccag gatcggcccg
    12661 agccccgtgc cgacgcccac cgaagcaaag agcagcccca tggaaatgcc gcccccgtga
    12721 ccgatggcat atacgcgctc ggccagcgtg accataatca cctggaaccc gccgttgcac
    12781 gtgaggaagt acagcggctt ctggagcgcc agcacgagga tgccgggatg cgcccgcaga
    12841 aagccgagac cggcgaggta ctgacgcagg accgcgccgg ggccgggcgc gtgcgcctgc
    12901 acctcggcct ccgcatggta gcggatgcgg gaaataaacg cggccgaggc aaggaatgtc
    12961 gccgcatcga tcaggaacgc gacgtgcagg ccgaacgttc ccgccaccag accgcccagc
    13021 gcggtgccga aggcgagcat cgacgaccat gtcgccgagc tgatcgcgtt ggccgcgccc
    13081 agttcgcgcg gcgtgacgag gctcggcaga atcgcgttgc gcgcggggaa gaagaagccc
    13141 gagatcccca attgcacggc cgtcagcacg tacaagagcc acacctgctc gggacgccgg
    13201 atgaacagaa acccgagaac caccaccgcc cggattagat ccgctgcgat cagcagatgc
    13261 ttgcggttga accggtccgc caccaccccc gccagcgggc tgaccgcgaa cggcgccagc
    13321 cagcgcacca ccagcagccc tccaaccgcg gcgcccgact gggtcaacat cccgatcagc
    13381 gtcgccgagg cgatcaggtt gaaccagtcg cccagcaggc tgacaaggtc gcccgaccac
    13441 aacaggcgat aattgcggtt tccgcgcagg acctccagat agccggcgcg cggcggggcg
    13501 ggatagccgg caacgggatc cgtcacggcg agggccattc ggtaacgctg ggcattgccg
    13561 tagccatatt ctttccatcc aacggctcaa cacgcccgaa ataagggcgc agaccttccc
    13621 atgatccgct gctggctggc ccctgcccag aagtaaatcg ccgggccgcg gcgatgagga
    13681 aaagttggcg gatgggcgga gggggaatat actgagctat tcatgccgtt tgcatcggtt
    13741 attagaaggg ggcccgctcg gcctccgccg tcgccgccca atcctcgcct tcgaacgatc
    13801 cccgtttccc gaagggagtc ccaccacgat gcatgccatg accgcctatc cgcccatcct
    13861 ccgcctcgcc ccgctggcgc tgctggccct cgccatgctg acgcccatgc ttccggcggc
    13921 cgaaattgaa tattacatga agatgtcgga cgtcgacggc gagtccgtgg acgatggaca
    13981 caaggactgg atcgtgttgc tctcgttcag ccacgcgatc aacaagcggg tggaggactt
    14041 cgtcaccacc ggccggtcga ccttcacgcc gatcgttgtc acaaagatcg tggaccggtc
    14101 ctcgccgctg ctgatgcggg ccattgcggg gggcaagctg ctgcccgccg tccagatcga
    14161 agcagttaat gccgagacgc agcagaccta cctgcgatac gaaatgaaga acgtcctgat
    14221 ttccaactgg agcacgtcgg gcaacgccga tatccagccg accgagcaac tctccctgaa
    14281 ctttgaggag atcaaggtga tctatcaccg ctacacgttc ggctcgcccg atcccgtcga
    14341 atcggtcgaa atgggctgga gtgtcatcga cgatgcggag gctccccgca agacggagta
    14401 aatccacgcc ccggcgagcg ccggccgccc ggccgcaccg accgggtccg tgtttcttcc
    14461 cgcactcaac ctgaacccta tgccagaaca ggagagcgac ccatgcagca tgcagcgacg
    14521 tgcaacggac gcctgatcct ctgggggttg ggccttcttt tcctcccctt cgcgccggtt
    14581 tttgcgcagg aaacctcccg tggatttctg tcgttgccgg gcgtcgaggg cgaaagcacc
    14641 gacgaattcc acaaggactg gatcgatgtg ctgtcggtcg atcatgcgat cgccaaactg
    14701 gtggaagact tcgccacgac cggccgatcc cggcacgcgc cgatattggt tcgcaaacgc
    14761 atcgacaagt cctcgcccag attgatgcaa gcctgcgcca atggaacaac gttgtccgag
    14821 gcggtcatcg atctggtgaa agcggaatcc ggcttgcgtt ttctacgaat ccgcctgacc
    14881 gatgtgctcg tctcgagtta ttccgtgggc gtcgcgaaag aagatgccct gggcctcccc
    14941 cgcgaggaaa tcaccttggt ctaccacaag attcaagtca cctacttccg ttactcgtcc
    15001 ggctcaaacg accccgtgga ggaagtcgag atgcaatggg atgccgtgaa cgacgtggag
    15061 gacaccggca agacggcgac cgaggaggaa acgctgcggc tggaatgacg gccgcttcgg
    15121 accggccggc ggcctttacc agcgcgcgtc gcgcaaccgc acgccccggc tgtagaggac
    15181 ctgatcctcg gcgtcgacga attgaatcat cagccgggtc gcgacggatt cggtcagttc
    15241 cggaacgtcg acgcgcatgc ggaccgcgcc gtgctcgtgc ggcgccagcg tgacgatctt
    15301 cgtctcgcgc tccaacgccc gaccgtcgcg ctcgatgcca tagcgcatgc gcagccggtt
    15361 caggttcagg aaatcgtagt ggttggagag cgtgaccgtc agacggcgcc gtcccggccg
    15421 cgtgatcagt tcgctttcat ggacgtgaat cggcgaataa accacgcggg tcgcccagta
    15481 ggcatccttc ggctgccgat cggccgccat cagcccttcg gggcgggcga cgccgccggt
    15541 cgcagcggtc cgcggcagcg gcgcgtcggg gctgagcggc acgaccagtt gtttcagatc
    15601 cagatcggcc agcgtccccg ccgtcccccc ggccaggtcg gcgcgcgctt caatcgtgcg
    15661 ccagcgttcg agccgttcct ccacgtcggg cgccggttcg ttcggttgcc gtagaaagaa
    15721 aaccggcatg gggcattcgt cggcgagtag cgccagcgcc tcggtcaccg gatacggcag
    15781 ggccagcacg tcggccagcg gcggcccctg caggaactcc aggtcggcct cgcgatcgct
    15841 cggcaccgcc agcgcgacca ggagcggccg cgaggggtcg agttcacgca ccagccgggc
    15901 ggtcgcctcg accaacggcg tccacgccgg ctcgccgccc ggcgaccaca ggatcaccga
    15961 cggatggagc cgcgccatac ggatcgtggc ctcgacgcgc gaacgcagct ccgccgcata
    16021 cggctcgacg acgagcgaat gcgccatgcc gccgaccggc atcgggcaga tgacgtagag
    16081 cccgaaccgg tcgcacagcg ccagaaactc cggatcgggc ggatgatcga tgatccgcac
    16141 caggttggcg ttggccagct tgagcagccg cacgtcgtgg cgcagctgct cttcggtcag
    16201 cgcccgaccg gtctccgggt gcagaccatg ccagtcgatg ccgcgcagcg tcaccttgcg
    16261 cccgttgacc tgaagcgcct ggttgacgat ccgcacctgg cgcagtccca cccagtgcgt
    16321 ctgcgtgtcg agcaggctgc cgccatgcat cagcttgagt tcgaggcggt agaggtgggg
    16381 cgtctcggct gtccagagcc gggggcggtc cacgatcagt tgcgcgcggc cgccgcgttc
    16441 gaaggaactg ggcgcttcga ccgaggctgc ggccagatcc accgttccgc tggccacccg
    16501 cgcacccgcg gggtcataca gctcgcagag gatatcgcag ccggccatcg cgcccgccgc
    16561 ggcgtaacga cgcgcgccgg cctcgaccgt caccagcgcc cgcgtgccgc cgcgcgtcaa
    16621 ctcggtcgtc accgcgggtt cggtcaggtg gaactccggg cgggccagga tccggacgtc
    16681 gcggaagagc cccgaaaggg cccaggcggc gtcggcgtcg aacggcatct cggggctcag
    16741 cttcgtcacc cgcacggcca gcaggttggc ggcgccgggc gtcacgtgct gggtgatgtc
    16801 gaagcgtttg ggcgtgtagg cgctgctgtc ctggccgacc aggcggccat tgacccaggc
    16861 ctggcagccc cagagcgcgc cttcgaactc cagaaagatc cggcggccgc gccaggcggc
    16921 cgggggggtg aactcgcgcc ggtacagccc cgcgctggcg gcgacgtcgc tcagccggct
    16981 cggttcgcgc cgccccggtt cctcatagcc ctgcagctcc caatggctcg gcacctggat
    17041 ctcgcgccag ccggaggcgt cgtattgcgg caggtagaat tgcgcgtcgg ccagttcgac
    17101 gccgccgggt ggctgcaact tgaagcgcca tgcgccgttg agcgattgcg acggcacgcc
    17161 gccgcgcgcc gcgccggcct ccagcggggc ctccggacgc agcagcgcgt aatccggcgt
    17221 ccccggcgtg gcgcaggcga gggcgaaaca gcaggtcaac agcggaaaca gtcggcgggc
    17281 ggtccataga agtgatcggc aggccatgag cgcgacaaca gctcctcaac gaaatgaacc
    17341 gtcatgcagg ttgccagatc catggagcag agaacagcgc gaactgtccc cggccgcctg
    17401 gacgtcgcgg gggcaccccg ggcggagcgc gcccggcgcc ggtcaccccg ccgccgcggc
    17461 gcccagatgc cacagcgcga gcagcggcga ccagatcaac cggggcaacc cggtgacgat
    17521 gtgatacagc gcgacgaatg gcgcagccag atcggccggc gccgtggcca gatcgaggac
    17581 gaacccgggt ggaaagaaaa ctttgaacgg cgccagcacg acggttccca ccgcgcgcaa
    17641 cggccgcagg aattgcggca gcgccagcgc cacatctgcc agatagagcg ccgggtcgag
    17701 cgccagccgg cccgcccggc cgacgtgcca cacggcgctc ggctccccgg ctggctcgct
    17761 gccgaccgcc tcggacagaa ccgcgccggc cggttcggtt gagtcttggt ctactgttgg
    17821 atgatgggcg cacccggcca gcaagagcag cgccgcgacg cggcagacca accggatcct
    17881 cacgcgcatg ccgtcgcggc cccctcgctc ggctctaccg gctgacggcc aatccaccca
    17941 ttacgtgttg gagacgagac gaccggcgaa gggcgtctcc gccagcagtt gttccatggc
    18001 ctcgcggttc ttcacgtcga gcaccggctc gttgtcatac tgttcgaaga acagcgtcgg
    18061 ctccagatcg agcaggccca gttccttgcg gatcagccgg tcgttgggga cgaacccgcg
    18121 cttcatcagc cgcaccaggg tgttgtgaat cgtgccgtaa agcccgcaga tgtagacgac
    18181 gcagcgcccc ggctccagtt cgcccggctt gagccccagg cgctcctcca gcacctccag
    18241 cttttcatcg tcgaagaagg tctccacgcg gccgacgtcg ccttcccatt cgggcgccat
    18301 ggccggccgg ctgatcgtcg gcatgtagcg ttcgggcagg ctcgcgaaca gccgccgcag
    18361 gtcgtcgcgg tagcccaggt cctgggggat gctggccccg tgcaggacgg cgaactggcc
    18421 cggcgacacc ccgcgcgtca cgtatgagag gatcatgctg acgaacggcg cgagccccgt
    18481 ccccgcggcg acgaagacct tcaggcgcgg gtcgttcgga tcgagcgtct tggccagcgt
    18541 gaagtgcccc gtgatcttct tgcccaggta gagccggtcg ccgaccttga gcggccagag
    18601 caggtgggtc agcgggttgt ccgactcggg ctgatcaaca taacggatgt aaaactcgag
    18661 gaattccttc tgctcggggg gcgaggcgat cgaatacggc cgctgcaccg aacccttctc
    18721 cggctcccgc tcgttgttca tcccgaggac gacgtattgg ccggcctcga aatcgggaat
    18781 ctggcccgcc gtggcgcccc acgaaggatc gggcttgacc cgcaggatgg agagcttggg
    18841 ggtcagatcc acccggtcgg tcagggtggc gttgtattcg agcagtttgg ccattggagg
    18901 tctgctttgc gaaagaacga tccggacctg tcggtccgac cccgcgtcga taggggcgga
    18961 tcatttgtat cacaggcggg ggcggattgc caacccgagg atcatcattt tccgcccccc
    19021 tcttcgggcc ggggcctcag atccccagcc ccggatcggc gttgagcgtg agcgccgcgc
    19081 gcggcgcgag cggttccaga tgccacgcca ggccggcgat catcgcggcg ttgtcggtgc
    19141 acagcagggg atggggaaac cagatgcgca gaccgggccg gtgggcggag agctcggcgg
    19201 cgcgccgccg cagcccgcga ttgcaggcga ccccgccggt gatgaccaga tcggcggccc
    19261 ggcgcgcggc gacggcatcg agcgccttgc ccagcagggc ctcgacggcg gcggcctgaa
    19321 acgcggcgca gaaattggcc agcgtccccg cgggcaactc gccgccgtgc tcggcgcgca
    19381 gccgttccag ctcgcgtccg gcggccgtct tcaggccgct gaaggagaag tccggtttgt
    19441 cgcggcgcat catcggcggc gccagatgga tgccgtcggg cgcgccggtc gccgccagcc
    19501 ggtcgatcgc cgggccgccg ggatagccca gaccggccac gcgcgcgacc ttgtcgtacg
    19561 cctcgccgac ggcgtcatcg cgcgtctggg cgatggtttc ggcgcgcgcg ggcgagccga
    19621 cccagaccag cgaggtgtgg ccgcccgaaa cgatcaggcc ga
//
LOCUS       DAOPCO010000044        19378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_2932_length_19378_cov_5.797340, whole genome shotgun sequence.
ACCESSION   DAOPCO010000044 DAOPCO010000000
VERSION     DAOPCO010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 19378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19378
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2932_length_19378_cov_5.797340"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..436
                     /locus_tag="PLS90_05300"
     CDS             53..436
                     /locus_tag="PLS90_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01853.1"
                     /translation="MIGIIVGLVAFGLIGCGRVHHRTTAAPGPISYSSVLPREYDVIQ
                     PFEESRRQVFLLFWLIPVKEGNGVQIAADQIRRTGADGAVNLQVNSSTDLLDFFVSLF
                     TVGLVNSWQVEAQGDLVRLREPAAM"
     gene            777..3446
                     /gene="mutS"
                     /locus_tag="PLS90_05305"
     CDS             777..3446
                     /gene="mutS"
                     /locus_tag="PLS90_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPK01854.1"
                     /translation="MNRETQLTPMVRQYQQIKAQHPDEILLFRLGDFYETFGDDARAT
                     AEILGITLTRKHIGQGRTLPLAGIPYHALDGYLAKLIRAGRRVAICDQVEDPKTAKGV
                     VKREVVRVVTPGTVIEEGLLEDKANNYLAAVARHQGNWGIAQAELSTGALAATEFSGD
                     DARAMLVNELARIGPAELLATEEDWELLDAEMRAAFGAGALLRTRIDKGMVRHGAARQ
                     ALLEHLGVATLQGYGAEDSPAAVAAAGAIVTYLTETQRRTLGHIRHLRIYSARDTMML
                     DATTQRSLELVRNLIDGGRRATLIDVLDWTRTPMGGRLLRQWLLQPLQDLEAIKARQD
                     AIEGLLGSAPAREQLGEALRGVRDLERIVARVHCGTANARDLVALGASLREVPAIKSA
                     LLRVGAGALRGLGEAIQTLAALADRLDSALLPEPPVTVREGGMIRDGVDPRLDELRAI
                     TRDGKGWLARMRAAEVEATGIASLKIGFNKVFGYYIEVSKANLDKVPAHYIRRQTLTG
                     AERFITQELKEKEEQILGAQDKIHDLEYEIFQSLIETVRGETPALQELAGQLAEVDVL
                     AALAEAALRGGYVRPEVDGSDLIEIRGGRHPVLEHLMTDAPFIANDVALDSKGEQIWL
                     ITGPNMAGKSTFIRQVALITLLAHVGSFVPARSARIGLVDRIFTRVGATDYLARGQST
                     FLVEMTETANILNNATDRSLVILDEIGRGTSTYDGLSIAWAVIEYLHNKPRRRARTLF
                     ATHYHELTELENRLERLANYNVHVREADDRITFLYQIVRGPADRSYGIYAAQLAGMPA
                     ETLDRAREILFQLECGRATPGDDGEGAGRKPDRPPALPLSRDVDIMQLDLFAPELPPV
                     FARLAQLDVNRLTPLQALQLLQELVEAAREFKN"
     gene            complement(3436..4176)
                     /locus_tag="PLS90_05310"
     CDS             complement(3436..4176)
                     /locus_tag="PLS90_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPK01855.1"
                     /translation="MHDAAKILPSPPEAVLLDMDGVLFDSEPIHKIAWRRILAGYGFE
                     FDDAFLQPWVGVPDHQLAAYLAGHGLVAEDPQRLLEIKRALYHKLTSERLRLFDGVPA
                     GLDALRERGLGLALVTASVRSDVRHVLEVVGLGDYFPVVVTGNDVERTKPAADPYLRA
                     AEGLGCRPQRCIVIEDSPNGIASARAAGAAVLAVMSSYPAEALAQADCVFPSTSLAIE
                     CVLARLDTDAREPARAPTDVSHGGGVSF"
     gene            4405..6657
                     /locus_tag="PLS90_05315"
     CDS             4405..6657
                     /locus_tag="PLS90_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01856.1"
                     /translation="MNHGKLDLDAILSKAEQGITAYLDAAKTAGHISPEQHKTAMANT
                     LPNLREWLTSPKIDELSPNLKAGVADAVEAGRWEDLVNAYRQSMRFGTGGIRGMMAFD
                     RESIRKLADDGLDARILKGPNTLNNVVLLRTSAGVAQFGKQAGRNFDRIVIGYDSRVR
                     GGDFARLIAELFLAYDYTVYLFDEPCPYPMVTFAIPHERIKAHVGILISASHNDYRYN
                     GYKLSCGNGSQFDPKERDEMYERYIAKATFDDIRLRPLSEAPRGKLYWLGGAEPVAGV
                     DYFGREDALINIHELHAAHVRGFLMTRDFSGMEPPLRIGYCPFHGAGRKAVPRLLNDI
                     GLTEIQRTTRNGLYELNGMFPSFCNDPGKEQQPDPGDPRAARIAVDAFKAEYPGDFEK
                     LDILIGTDPDADRCGVVVKVPENQRRLHGGQDWMLLPADDMWGLLLWYRFHREAEAAG
                     GRVPDADKKFAVLSHTTTDAIVRLCRKFGVGVIKTWVGFAALSAAVRDQWDKVPQQNL
                     VDGRVQPDDPLCHPYICEYIDMHAGRTINIGSMEQSNGFSLLGGPPPDPFSLGVNGHV
                     RDKDGVFAAILSAEVAAWAKQQGTSLFELVDRHIYLDPAIGLFVNHYEPDPLDGEYPG
                     IEGDRKKIGILKIALDLYRAQREGKPVHIGGKDVVATVMYRTGKYDRIYPPADGFVFP
                     DEGVRLFFSADRLDHLTIRPSGTGNSLRFHIQLHSAVEEANLIERKAELRRQAAAIAD
                     HIREILGAPR"
     gene            7286..7347
                     /locus_tag="PLS90_05320"
                     /pseudo
     CDS             7286..7347
                     /locus_tag="PLS90_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            7394..7672
                     /locus_tag="PLS90_05325"
     CDS             7394..7672
                     /locus_tag="PLS90_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017354562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HPK01857.1"
                     /translation="MPGSLPNYLVQAILVTVCCCVPFGIVAIVYAAQVNSKLTAGDFA
                     GATESSAKAKKWCWIALITGLIVNGLVVALQVAGILAGVGMEAAGGGM"
     gene            complement(7744..9126)
                     /gene="odhB"
                     /locus_tag="PLS90_05330"
     CDS             complement(7744..9126)
                     /gene="odhB"
                     /locus_tag="PLS90_05330"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HPK01858.1"
                     /translation="MATEIRVPQMGESVVEATISRWLKKEGDRVNVGDPLLELETEKV
                     NMEVGAEKAGVLQEILHGDGSDVRVGEVIGILADAVEAEPAAPGAAQTPAEPSGEEKE
                     PAPTVAPAEPAGSGRAKADKPSEREPLRRLAHGEKPGAAPKPQPPAEETRDYEPPRIR
                     RDELLSGESSFRSPAEARAPREGVGGPPRALGAGGGSMRDHMEQYLAGQERRTAPPQR
                     PPRPEAPAPGGREERIRMSRRRRTIARRLVEAQHDAAMLTTFNEINMAAVSDVRKRWR
                     EAFEQRHGVRLGFLSFFAKATVSALREFPHLNAEIEDDDLIIKHYYDIGIAMDTPEGL
                     VVPVLRGVDKLPFAGIEKEITEFARKARGGELTLDDLRGGTFTITNGGVFGSMLSTPI
                     LNPPQVGILGLHRITERPVAEHGEVVIRPMMYVALSYDHRVVDGRVAVQFLVRIKEMI
                     EDPERLLLEG"
     gene            complement(9181..11982)
                     /locus_tag="PLS90_05335"
     CDS             complement(9181..11982)
                     /locus_tag="PLS90_05335"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HPK01859.1"
                     /translation="MNIWEDFHGSNAGYVLELYDRYQQDPLSVDPETRKFFDNWTPDS
                     SAAPPDLALPIARVVGAVNLANSIRWFGHLDAQLDPLGIAPPPGDPSLDPAFHGISNE
                     DLRDLPASLIGGPIAQRAESALDAIEMLRAVHSYHTGFDFDHVHNPEEREWLAYKAES
                     AFYRPPNVPIDRKALLRRLTEVEVFENFLHRVFPGKSRFSIEGLDMLVPMLDEVLCMA
                     ADAGINRILIGMAHRGRLNVLAHVLNKSYAQILAEFKDPVTERDFLDDLSWTGDVKYH
                     RGASRALGGEEWIGLEVSIAPNPSHLEHVNPVVQGMARAAASRVDRAGPPIVDPTYTL
                     PIVIHGDAAFPGQGINAETLNLSRLNGYMTGGTIHIITNNQLGFTTTPTDARSTLYAS
                     DLAKGFEIPILHVNADDPEACIEAARIATAYRMRFKKDFVIDLIGYRRHGHNEGDEPF
                     FTQPRIYRVIEQHPTVRQIWADALVRRCDIIPDWAEGLVRQQMEKLQHVYEGFSPEES
                     LVEPHSKVPPPGAARRVRTQVPLERLVSALEALMALPEGFTIHPKLEKGVQRRIAAAH
                     NPEQRLVPWGMAEELAFATVLEDKIAIRLTGEDVERGTFNQRHAVYHDYNTGERFVPL
                     QNIPQARAAFEICNSPLTENAALGFEFGYNIQEPSRLVLWEAQYGDFINTTQAMIDEF
                     LVSARAKWGQTPSLVLLLPHGYEGQGPDHSSARLERFLQLAADTNMRIAYPTTAAQYF
                     HLLRRQALLLKTDPLPLIVMSPKSLLRHPATASSLKEIAERQWQPVLDDPRPELPPEG
                     VRRLVFCSGKIAVDMLHHDGKDAGNPAVAVVRLEQLYPFPRPDLEQVYARYPNLQEVV
                     WLQEEPENMGAWEFARPRLNDMLGERMPLNYMGRYRSASPAEGSHAWHVATQRMLLDR
                     AWNVEEPARAENAVLIKTV"
     gene            complement(12146..13570)
                     /gene="lpdA"
                     /locus_tag="PLS90_05340"
     CDS             complement(12146..13570)
                     /gene="lpdA"
                     /locus_tag="PLS90_05340"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPK01860.1"
                     /translation="MAGDKAQVVVIGAGPGGYAAAFQAATLGLEVTLVNAEPDPGGVC
                     LHRGCIPSKALLHVARLINESREAEVCGLSFGKPKIDLDALRAWKSQVVKRLAGGVRQ
                     IAGARKVRYLAARASFLDSTTLALAPLEGAEGVPERLSFEHCILASGSRPAIPGPFQI
                     DDERIMNSTGALNLPDVPKKLLVIGGGYIGLEMGTIYQALGSEVTVVELTDGLLPGAD
                     RDLVKPLHKRLEESFKAIHLKTRVEGLEPTKQGIKVKLSGEGAAPEMLFDRVLIAVGR
                     RPNSEGLGLERTGVQLTEKGFVKVDRQQRTGDERIFAIGDVAGEPMLAHKASREAKVA
                     AEVIAGHRSAFEVAAIPAVVFTDPELAWAGLTENEAAARGIAVKVARFPWAASGRAAT
                     LGRTEGLTKLVIEPESERVLGVGMVGPNAGDMIAEGALAIEMAALARDVADTIHPHPT
                     LSETLMEAAELYFGQATHMVGGKR"
     gene            complement(13911..15299)
                     /locus_tag="PLS90_05345"
     CDS             complement(13911..15299)
                     /locus_tag="PLS90_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPK01861.1"
                     /translation="MATEFKLPEVGENIEKATVIRVLVNEGDTVKKEQTLVEIETDKA
                     TIEVPSTVTGKVGKIKVKEGESVPVGATLMTIAEEGEAGESRPARAEKAPKEEPPVSR
                     KQEARRAEEQRETAAAEEAPEPEEDSRERKAVPRREARVEQLTPAARKAPPAPGQTVP
                     ASPSVRRLAREIGVDISEVEGTGPGGRISEDDVKAHARRQAGGAPRPARAAARPAVSV
                     SADLPDFSRWGEIEAEPLNNIRRVISERLSLSWQTIPHVHHHDKADITELEELRRRRN
                     AAIDDPARKLTVTAVLLKVLGKLLRRHPKFNASLDAANNQLILKHYCHIGVAADTERG
                     LLVPVVRDVDRKSISELVADLHALAGRAREGKLSPEEMSGGTFTVSNLGGIGGVGFTP
                     IINLPEVAILGVSRAAYEQVFDREGNGRVRLLLPVCLGYDHRVIDGAEAARFVREMCE
                     LLTSPFDMLFEI"
     gene            complement(15342..18035)
                     /gene="aceE"
                     /locus_tag="PLS90_05350"
     CDS             complement(15342..18035)
                     /gene="aceE"
                     /locus_tag="PLS90_05350"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HPK01862.1"
                     /translation="MYEYAPENEHELDEQETLKAIEDQEWMDSLEDVLVRQGPERVRE
                     LIGKLRDFAYSNGVVLPFSANTPYVNTIPVSQQPPFPGSRERERRIKSIIRWNAMAMV
                     VRANKASAGIGGHISTYASSATLYEIGFNHFFRARRDDFSGDQIYFQGHASPGIYARA
                     FLEGRISEEHLMNFRRELAEGGGLSSYPHPWLMPNFWQFPTVSMGLGPIMAIYQARFN
                     RYLEDRGIKNTSESKVWCFMGDGECDEPESLGAITLAAREKLDNLIFVINCNLQRLDG
                     PVRGNGKIIQELEAAFRGAGWNVIKVIWGSDWDELLERDESGLLVQRMNELIDGEAQK
                     LVVEDGAYVREKFFGKYPELLKLVENHTDESLRKLRRGGHDPVKVYAAYKAAVEHKGA
                     PTVILAQTVKGYGLGEAGEGRNITHQQKKLNEDELREFRTRFGIPISDEEVAKAPFYK
                     PPDDSPEMQYLRERREALGGHQPQRHPQTEAFPPLDPELFTEFHSGDEREVSTTMAFV
                     RLLAKLMRQEGFGKRIVPIVPDEARTFGMEALFRQFGIYSHVGQLYEPVDASSLLYYK
                     EATDGQILEEGITEAGSMSSFIAAGTAYATHGVNMLPFFIFYSMFGFQRIGDLIWAAG
                     DCRCRGFILGGTAGRTTLNGEGLQHEDGNSQLLAYPLPNLKAYDPAFAFEIAVIIEDG
                     IRRMYVEQEDIFYYITLYNENYSQLPMPEGAREGILKGMYLLREAPKKKLKHKINLMG
                     GGSLINEALKAQQLLAEQYGVAARVFSVTSYKELYYDAEQCVRWNRLNPEKKPRKPYV
                     TQLLEKGPNVTVAVSDYVKALPESIGRFIPGRFHPLGTDGFGRSESRAALRDFFEVDS
                     RHVALAALTELVAEGELEPGVLSQAIKDLGIDPAKPNPMVS"
     gene            18544..18897
                     /locus_tag="PLS90_05355"
     CDS             18544..18897
                     /locus_tag="PLS90_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HPK01863.1"
                     /translation="MHEIIAIQPLGDFKFDLESADGTRGTVALSNLRGKGVFKLWDEP
                     GAFEAVKIGSSGELVWHDRIDLCPGALCLRVTGKLPEEGFPGKIPLMPELSCCFGIII
                     KMFFDDHAPPHFHAE"
     gene            19018..>19378
                     /locus_tag="PLS90_05360"
     CDS             19018..>19378
                     /locus_tag="PLS90_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017783429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01864.1"
                     /translation="MHHAAMLWTGGKDCALALDEAIRTGIDIRCLATFAPPRPDFLAH
                     PLALMQLQARAMGLPHHIVAVTAPFDQGYETGLARLRDEFGIDGVVTGDISEVDGCPN
                     WIRERCRPVGLDVLTPLW"
BASE COUNT         3103 a         6893 c         6051 g         3331 t
ORIGIN      
        1 gcacccggtg cggatctcgg tggagtgagg tgcgatgatg ctgagaagcg caatgatcgg
       61 aatcattgtc gggctggtgg cattcggtct gatcggctgc ggccgggtgc accaccgcac
      121 gacggccgcc ccggggccga tcagctacag ctccgttctg ccccgggagt atgacgtcat
      181 ccagccgttc gaggaatcgc gccggcaggt gtttctgctc ttctggctga tcccggtcaa
      241 ggaggggaac ggcgtgcaga tcgctgccga tcagatccgg cggacggggg ccgacggcgc
      301 ggtcaacctg caggtcaatt cgagcaccga cctgttggat ttcttcgttt cactgttcac
      361 cgtcgggctg gtcaattcat ggcaggtcga ggcgcaaggc gatctcgtcc ggttgcgcga
      421 accggccgcg atgtgacgcg gaaagagacg agggacggtt ccctcacctt tcgtgtccct
      481 cgtcctcgta atcgctccaa aagatcttct cccaaaggtc ttctctcaaa atttggtccc
      541 ggctctcgtc cctcgtcctc ggctctcgaa ttcgtggaga accgccggtt cacgcgtagt
      601 tcgtcatcgg tcggtccccg ctcttccctc gcccctgatt ccgctcccat ccttgctccc
      661 gtcgccgcgc ggcgttacag tagcgcctcc gaatccagtt cggacgcgcg gcgcttcccc
      721 ctcccagcca tccggcgccg cccgacctct ccccgcccca ccggagccgc gccgccatga
      781 accgcgagac ccagctcacg ccgatggtcc gccagtatca gcagatcaag gcgcagcacc
      841 ccgatgaaat tctgctcttc cggctggggg atttttacga aaccttcggc gacgacgctc
      901 gcgccaccgc cgaaatcctc ggcatcacgc tcacccgcaa acacatcggc cagggccgca
      961 cgctcccgct ggccggcatc ccgtatcatg cgctcgacgg ttacctggcc aagctgatcc
     1021 gcgccggccg ccgcgtcgcg atctgcgatc aggtggagga ccccaagacg gccaaggggg
     1081 tcgtcaagcg cgaggtcgtg cgcgtcgtca cgcccgggac ggtcatcgaa gaggggctgc
     1141 tcgaggacaa ggcgaacaat tacctggcgg ccgtggcgcg ccaccagggc aactggggga
     1201 tcgcgcaggc ggaactatcg accggggcgc tggccgccac cgaattcagc ggcgacgacg
     1261 cccgcgcgat gctggtcaac gagctggccc gcatcggccc ggccgagctg ctcgccaccg
     1321 aggaggactg ggaactgctc gacgcggaga tgcgcgcggc cttcggcgcc ggcgcgttgc
     1381 tgcgcacgcg catcgacaag gggatggtgc gccacggcgc cgcgcgccag gcgctgctgg
     1441 agcacctggg ggtcgcgacg ctccagggtt acggcgccga ggattccccc gccgccgtcg
     1501 ccgccgccgg cgcgatcgtg acctacctga ccgagacgca gcggcgcacg ctgggccaca
     1561 tccgccacct gcgcatctat tcggcgcgcg acacgatgat gctcgacgcg acgacgcagc
     1621 ggtcgctgga actggtgcgc aacctgatcg acggcggccg ccgcgccacg ctgatcgacg
     1681 tcctcgattg gacccgcacg ccgatgggcg gccgcctgct gcgccagtgg ctgctccagc
     1741 cgctgcagga cctggaggcg atcaaggcgc gccaggatgc gatcgagggg ctgctgggtt
     1801 cggcgcccgc gcgcgaacag ctcggcgagg cgctgcgcgg cgtacgcgat ctggagcgca
     1861 tcgtcgcccg cgtccactgc ggcacggcca acgcgcgcga cctggtcgcg ctgggggcgt
     1921 cgctgcgcga ggtccccgcg atcaagtcgg ccctgctgcg cgtcggcgcc ggcgcgctgc
     1981 gtggactcgg cgaggcgatc cagacgctgg ccgcgctggc cgaccgcctc gacagcgcgc
     2041 tgctgcccga gccgcccgtc accgtccgcg agggcgggat gatccgcgac ggcgtcgatc
     2101 cccggctgga cgagctgcgc gcgatcacgc gcgacggcaa gggctggctc gcccgcatgc
     2161 gcgccgccga agtcgaagcc accggcatcg cctcgctcaa gatcggcttc aacaaggtct
     2221 tcggctacta tatcgaggtc agcaaggcca acctggacaa ggtgccggcg cactatatcc
     2281 gccgccagac gctcaccggc gccgaacgct tcatcaccca ggaactcaag gagaaggaag
     2341 agcagatcct cggcgcgcag gacaagatcc acgacctgga atacgagatt ttccagagcc
     2401 tgattgagac ggtgcggggt gaaacgcccg ccctgcagga actggccggc caactggccg
     2461 aggtcgacgt gctcgccgcg ctggccgaag ccgcgctgcg cggcgggtat gtgcggcccg
     2521 aggtggacgg ctccgacctg atcgaaatcc gcggcggccg ccacccggtg cttgagcacc
     2581 tgatgaccga cgcgccgttc atcgccaacg acgtggcgct cgattcgaaa ggcgagcaga
     2641 tctggctcat caccggaccg aacatggcgg gcaagtcgac gttcatccgc caggtggcgc
     2701 tgatcacgct gctggcgcac gtcggctcgt tcgtgccggc gcggtcggcc cgcatcggcc
     2761 tggtcgaccg gattttcacc cgcgtcggcg ccaccgacta tctcgcgcgc ggccagtcga
     2821 cgttcctcgt cgagatgacc gagacggcca acatcctcaa caacgccacc gaccgcagcc
     2881 tggtcatcct ggacgaaatc ggccgcggca cctcgaccta cgacggtctc tcgatcgcgt
     2941 gggccgtcat cgaatacctg cacaacaagc cgcggcgccg ggcgcgcacg ctcttcgcca
     3001 cgcactatca tgaactgacc gaactggaaa accggttgga gcgactcgcc aactacaacg
     3061 tccacgtgcg cgaggccgac gaccggatca cgtttctcta ccagatcgtg cgcggccccg
     3121 ccgaccgctc gtacgggatc tacgccgcgc aactggccgg catgccggcc gagacgctcg
     3181 accgcgcacg cgaaatcctg ttccagctgg agtgcgggcg cgccaccccc ggcgacgatg
     3241 gcgagggcgc cggccgcaaa cccgatcgcc cgccggcgct ccccttgagc cgcgacgtcg
     3301 acatcatgca gcttgacctg tttgcgcccg agctgccgcc ggtcttcgcg cgattggcgc
     3361 agctcgacgt caatcggctg accccgctgc aagccctgca actgttgcag gaactggtcg
     3421 aggcggcgcg ggagttcaaa aactgacgcc gccgccgtgt gagacgtccg ttggcgcccg
     3481 cgcgggttcc cgcgcatccg tgtccagccg tgccagcacg cattcaatcg ccagcgatgt
     3541 cgaagggaag acgcagtcgg cctgcgccag cgcttcggcc gggtacgacg acatcaccgc
     3601 cagaaccgcc gccccggccg cgcgggccga ggcgatgccg ttgggcgaat cctcgatcac
     3661 gatgcatcgc tgcggccggc aacccagccc ctccgccgcg cgcagatacg gatcggcggc
     3721 gggctttgtt cgctccacgt cgttgccggt gacgacgacc ggaaagtaat cgcccagacc
     3781 gacgacctcc agcacgtgcc gcacgtcgct gcgcacgctg gccgtcacca gcgccaggcc
     3841 cagccctcgt tcgcgcagcg cgtcgagccc cgccggcacg ccgtcgaaca gccgcagccg
     3901 ctcgctcgtc agtttgtgat acagcgcgcg cttgatctcc agcagccgct gcgggtcctc
     3961 cgccaccagc ccgtgaccgg ccagataggc cgccaactgg tggtccggca cgccgaccca
     4021 cggttgcagg aaggcgtcgt cgaactcgaa cccgtagccg gccaggatcc ggcgccaggc
     4081 gatcttgtgg atcggctcgg aatcgaacag aacgccgtcc atgtcgagca gcaccgcctc
     4141 cggcgggctg gggaggatct tcgccgcgtc gtgcatagga cgctcctttc gcggccagtc
     4201 tagcatcccg gtcgcctccg ctccacgccg catccgaatc ggattcggta tcggtatcgg
     4261 aatcgcattg tcgctatcgt gatcgcccca ttcccgctct ccccggaatt tgaccttccc
     4321 taatccccgg ccaccggcta aaaaggaatt ccgcgtgtct gggcgacggc tacgacgaaa
     4381 acgacccgac cgaaaggacc tccaatgaat cacgggaaac tcgatctgga cgccatcctg
     4441 agcaaggccg aacagggaat caccgcctat ctggacgccg cgaagaccgc cggccatatc
     4501 agccccgagc agcacaagac cgccatggcc aacacgctgc ccaacctgcg ggagtggctg
     4561 acctcgccga aaatcgacga actttccccc aacctgaagg ccggcgtcgc cgacgccgtc
     4621 gaagccggcc gctgggagga tctggtcaac gcctatcgcc agtcaatgcg cttcggcacc
     4681 ggcggtatcc gcggcatgat ggcgttcgac cgcgaatcga tccggaaact ggctgacgac
     4741 gggctcgacg cgcgcatcct caagggcccc aatacgctca acaacgtcgt cctgctgcgc
     4801 acgtcggcgg gcgtcgccca gttcggcaag caggccggcc gcaacttcga ccgcatcgtg
     4861 atcggctacg acagccgcgt ccgcggcggc gacttcgcgc gtctgatcgc cgagctgttc
     4921 ctcgcctacg attacaccgt ctacctgttc gacgagccgt gcccctaccc gatggtcacg
     4981 ttcgccatcc cgcacgagcg gatcaaggcc cacgtcggca tcctgatctc ggccagccac
     5041 aacgattatc gctacaacgg ctacaagctc tcgtgcggca acgggtcgca gttcgacccc
     5101 aaggagcgcg acgagatgta tgagcgctac atcgccaagg cgacgttcga cgacatccgg
     5161 ctccgcccgc tgagcgaagc gcccaggggc aagctttact ggctgggcgg agccgaaccg
     5221 gtcgccggcg tcgattactt cggccgcgag gacgcgctga tcaacatcca cgaactgcac
     5281 gccgcgcacg tgcgcgggtt cctcatgacc agggatttca gcgggatgga accgccgctg
     5341 cggatcggct actgcccgtt ccacggcgcc gggcgcaagg ccgtgccgcg gctgctgaac
     5401 gacatcggcc tgaccgagat ccagcgcacg acccggaacg gcctctacga actcaacggg
     5461 atgttcccca gcttctgcaa cgaccccggc aaggagcagc agcccgatcc gggcgacccg
     5521 cgcgccgccc gcatcgccgt cgacgcgttc aaggccgaat accccggcga ctttgaaaaa
     5581 cttgacatcc tgatcggcac cgatcccgac gccgaccgct gcggcgtcgt cgtcaaggtc
     5641 cccgaaaacc agcgccggct ccatggcggc caggactgga tgctgctgcc ggccgacgac
     5701 atgtggggcc tgctgctgtg gtatcgcttt caccgcgaag cggaggccgc cggcggccgt
     5761 gtccccgacg ccgacaagaa gttcgccgtc ctgtcgcaca cgaccaccga cgccatcgtg
     5821 cggctctgcc gcaagttcgg cgtcggcgtg atcaagacct gggtcggctt cgccgcgctg
     5881 tcggccgccg tccgcgacca gtgggacaag gttcctcagc agaacctcgt cgacggccgc
     5941 gtccagccgg acgatccgct ctgccacccc tacatctgcg aatacatcga catgcacgcg
     6001 ggacggacga tcaacatcgg ctcgatggag cagtccaacg gcttcagcct gctgggcggc
     6061 ccgccccccg acccgttcag tctgggggtc aatggccatg tccgcgacaa ggatggcgtc
     6121 ttcgctgcga tcctgtcggc cgaagtggcg gcctgggcca agcagcaggg gaccagcctg
     6181 ttcgagctgg tcgaccggca catctacctc gaccccgcga tcggcctgtt cgtgaatcac
     6241 tacgaaccgg acccgctcga cggcgaatac ccgggcatcg agggagaccg caagaaaatc
     6301 ggcatcctca agatcgccct cgacctgtat cgcgcgcagc gggagggcaa gccggtccac
     6361 atcggcggca aggacgtcgt cgcgaccgtg atgtatcgca cgggcaaata cgaccggatc
     6421 tatcccccgg ccgacgggtt cgtcttcccc gatgagggcg tgcggctgtt cttcagcgcc
     6481 gaccggctgg atcacctgac gatccgcccc tcggggaccg gcaactcgct gcggttccac
     6541 atccagcttc atagtgcggt ggaggaagcc aacctgatcg agagaaaggc cgaactgcgc
     6601 cggcaggccg cggccatcgc cgatcacatc cgcgaaattc tcggcgcgcc gcggtagaac
     6661 gagaggcgat gacatttgcc gaagactgat atgaaaggag gaatccgcgg accggacgac
     6721 cgcacaactc ccaccgagca cgaaatgtca tccgattgca ccggcctcag gccgtacggt
     6781 gtttcattgt tttcaggtca tgcacaagct cggcaacgga actgccgacg aacggatata
     6841 tcaaaagggg aatgcctcgc gtcggtcccg cgcttcagga gccttctctt gttcctcttg
     6901 tttccgacat ggggtcacgc ttccccggat gccaaaccct ccacatccac ccccaccctc
     6961 cgcatcgcca cctgacgccg gagtcggctg cggccatccg gcagtccctc agcgaaatgg
     7021 atcccgaacg acaccatacg cacggcgtcg gcgccggcgg gggaagcgtt tgcctccgcg
     7081 gtggttcctg aatcagagtg aagatcgcga aatcgcggag gtgagcggcg gaaaacagat
     7141 tgcgcatcgc acgcccatcg gcgatgatcc gagcattcgt tccgtcgcaa tgagcggtgc
     7201 ggtgatcggc gacgcgcaac ccggcagccg tcggcgcggg cagggcacga ctaccgccca
     7261 cattcaaccg taaaggatcc gaattatgtt ctgcccccaa tgcggcgccg aaaaccccga
     7321 aggttcgcag cgctgctcgc aatgcggcgc gatcctgccg cagcgccagc cggcgccggc
     7381 cggcgctgcg cccgtgcccg gatcgcttcc gaactatctc gtgcaggcaa ttctcgtcac
     7441 cgtctgctgc tgcgttccgt tcggaatcgt ggccatcgtc tacgccgcgc aggtgaacag
     7501 caagctgacc gcgggggact tcgcgggggc gaccgagtcg tcggccaagg cgaagaaatg
     7561 gtgctggatc gcgctgatca ccggcctgat tgtcaatggc ctcgtcgtcg cccttcaggt
     7621 cgcggggatt ctcgccggcg tcggcatgga ggccgccggc gggggaatgt gagagagccg
     7681 agaacgagag ccgggagccg aattctgggt agccctatgg tggacgatag ggtggcgagc
     7741 ctctcagcct tccagcagca gccgttcggg gtcctcgatc atctccttga tccgcaccag
     7801 aaactgcacg gcgacgcgtc cgtcgaccac acggtggtca tagcttagcg ccacatacat
     7861 catcggccgg atcaccacct cgccgtgctc cgccaccggc cgctccgtga tccggtgcag
     7921 gccgaggatc cccacctgtg gcggattgag gatcggcgtg ctcagcatgc tgccgaaaac
     7981 gcccccgttg gtgatcgtga aggttccccc gcgcagatcg tcgagcgtca gttctccgcc
     8041 gcgcgccttc cgcgcgaatt ccgtaatctc cttctcgatc ccggcgaacg gcagcttgtc
     8101 caccccgcgc agcaccggca cgacaagccc ctcgggcgta tccatcgcga tcccgatgtc
     8161 gtagtaatgc ttgatgatca ggtcgtcgtc ctcgatctcg gcgttgaggt gggggaattc
     8221 gcgcagggcg ctgaccgtcg ccttggcgaa gaacgagagg aaccccaggc gaacgccgtg
     8281 gcgctgttcg aacgcctcgc gccagcgttt gcggacgtcg ctcaccgccg ccatgttgat
     8341 ctcgttgaac gtcgtcagca tcgccgcgtc gtgctgcgcc tcgacgagcc ggcgcgcgat
     8401 cgtccgccgc cggcggctca tgcggatgcg ctcctcgcgc ccgccggggg ccggcgcctc
     8461 cggccgcggc ggccgctgcg gcggggccgt tcgccgttcc tgacctgcca ggtactgctc
     8521 catgtgatcg cgcatcgagc cgccgccggc gccgagggcg cggggcggac cgccgacgcc
     8581 ttcgcgcggg gcgcgcgcct cggccggcga ccggaagctt gattccccgc tcagcagctc
     8641 atcgcggcgg atccgcggcg gctcgtaatc gcgcgtttcc tccgcgggcg gctggggctt
     8701 cggcgccgcg ccgggttttt cgccatgcgc caggcggcgc agcggctccc gctcgctcgg
     8761 cttgtccgcc ttcgcgcgcc cggacccggc cggctccgcc ggagcgacgg tcggcgccgg
     8821 ttccttttcc tcgccgctcg gctcggccgg ggtctgcgcc gcgcccggcg ccgccggctc
     8881 ggcctcaacg gcgtccgcca ggatgccgat cacctcgccc acgcgcacgt cgctcccgtc
     8941 gccgtgcagg atctcctgca gcacgccggc cttttcggcg ccgacctcca tgttgacttt
     9001 ctcggtctcc agctcaagca gcggatcgcc gacgttgacg cgatcccctt ccttcttgag
     9061 ccaacgcgaa atcgtggcct cgaccaccga ctcgcccatc tgcggcaccc ggatttcagt
     9121 cgccatggat caatgctttc cctcggaatt ggaccggcat cggcctccgc cggaccttgc
     9181 ttacaccgtc ttgatcagca ccgcgttctc ggcccgcgcg ggttcctcca cgttccatgc
     9241 ccggtccagc agcatccgct gcgtcgccac gtgccacgcg tgcgaacctt cggccgggct
     9301 ggccgaacgg tagcggccca tatagttgag cggcatgcgc tcgcccagca tgtcgttcag
     9361 ccgcggccgc gcgaattccc acgcccccat gttctccggc tcctcctgca gccacaccac
     9421 ctcctgcagg ttgggatagc gggcatagac ctgctccagg tcgggccggg ggaacggata
     9481 gagctgctcc agccggacga ccgccacggc cgggttgccg gcgtccttcc cgtcgtgatg
     9541 cagcatgtcg acggcgatct tgccgctgca gaagacgagt cggcgcactc cctcgggggg
     9601 cagctccggg cgcggatcat cgagcaccgg ctgccactgc ctctcggcga tctccttgag
     9661 cgacgacgcc gtcgccgggt ggcgcagcag gctcttgggc gacatcacga tcagcggcaa
     9721 cgggtcggtc ttgagcagca gcgcctggcg ccgcagcagg tggaaatact gcgccgccgt
     9781 ggtgggatag gcgatgcgca tgttggtgtc ggccgccaac tgcaggaagc gctcgagccg
     9841 cgcgctggaa tggtcggggc cctggccctc gtagccgtgc ggcagcagca ggacgagcga
     9901 aggagtctgg ccccatttgg cccgcgccga gacgaggaac tcgtcgatca tcgcctgcgt
     9961 cgtgttgata aagtcgccgt actgcgcttc ccacagcacc agccggctcg gctcctggat
    10021 gttgtagccg aactcgaacc cgagcgcggc attttcggtc agcgggctgt tgcagatctc
    10081 gaacgccgcg cgcgcctgcg ggatgttctg cagcgggacg aaccgctcgc ccgtgttgta
    10141 gtcgtgatag acggcatggc gctggttgaa cgtgccgcgc tcgacgtcct cgccggtcag
    10201 gcggatcgcg atcttgtcct ccagcaccgt cgcgaaggcc agttcctcgg ccatgcccca
    10261 ggggaccagg cgctgctcgg gattgtgcgc cgccgcgatc cgccgctgga cccccttttc
    10321 cagtttgggg tggatcgtga agccctcggg cagcgccatc agggcttcca gcgcactgac
    10381 cagccgctcc agcggaacct gcgtgcgcac gcgccgcgcc gcgcccggcg gcggcacctt
    10441 cgagtgcggc tcgacgagcg actcctcggg ggaaaatccc tcgtaaacgt gctgcagctt
    10501 ctccatctgc tggcgcacca gcccctcggc ccagtcgggg atgatgtcgc accgccgcac
    10561 gagcgcgtcg gcccagatct gccgcacggt ggggtgctgc tcgatcacgc ggtagatccg
    10621 cggctgcgtg aagaacggct cgtccccttc gttgtgaccg tgccggcggt agccgatcag
    10681 gtcgatcacg aagtccttct tgaagcgcat ccgataggcc gtggcgatcc gcgcggcctc
    10741 gatgcacgcc tcggggtcgt ccgcattgac gtgcaggatc gggatctcga agcccttggc
    10801 caggtcgctg gcgtacagcg tgctgcgcgc gtcggtcggc gtggttgtga aaccaagctg
    10861 gttgttcgtg atgatatgga tcgtgccgcc ggtcatgtag ccgttcagcc gcgacaggtt
    10921 gagcgtctcg gcgttgatcc cctggccggg gaacgccgcg tcgccgtgga tcacgatcgg
    10981 cagcgtgtag gtcgggtcga cgatcggcgg accggcccga tcgacgcgcg acgccgccgc
    11041 ccgcgccatc ccctgcacga ccgggttgac gtgctccaga tgacttgggt tgggggcgat
    11101 cgaaacttcc agcccgatcc actcctcgcc gcccagcgcg cgcgacgccc cgcggtgata
    11161 cttgacgtcg cccgtccacg acaggtcgtc gaggaagtcg cgctcggtca ccgggtcctt
    11221 gaactcggcc aggatctgcg cgtagctctt gttgagcacg tgggccagca cgttgagccg
    11281 gccgcggtgc gccatgccga tcaggatccg gttgatgccc gcgtcggccg ccatgcacag
    11341 cacctcgtcc agcatcggga cgagcatgtc gagaccctcg attgagaacc gcgacttgcc
    11401 ggggaaaacc cggtggagga aattctcgaa gacctcgacc tcggtcagcc ggcgcagcag
    11461 cgccttgcgg tcgatcggca cgttcggcgg gcggtagaac gccgattcgg ccttgtaggc
    11521 cagccactcg cgctcctccg gattgtgtac gtgatcgaag tcaaatccgg tgtggtacga
    11581 atgcacggcg cgcagcatct cgatcgcgtc gagcgccgac tcggcccgct gggcgatcgg
    11641 gccgccgatc aggctggccg gcagatcgcg caggtcctca ttcgagatgc cgtgaaacgc
    11701 cggatcgagc gaggggtcgc ccggcggcgg cgcgatcccc agcggatcga gctgcgcgtc
    11761 caggtgcccg aaccagcgga tcgagttggc gagattgacg gcgccgacaa cccgcgcgat
    11821 cggcaacgcc agatcggggg gcgcggcgct cgagtcgggc gtccaattgt cgaaaaactt
    11881 ccgcgtctcg ggatcgaccg acagcggatc ctgctgatag cgatcataca gctcgagcac
    11941 atagccggca ttcgaaccgt gaaaatcttc ccaaatattc atgatgggtt ctcgtgaggg
    12001 aacgaccgga ccggtgacgg gcggggacca gtcagacggg cggccgcggc cgtgactttc
    12061 tgcttcctct gtcgtcggtt ctcaccaggc tccgccggca cgcgatcgcc tggacatctc
    12121 gaacaaaccc gcgacggtcg gcggctcagc gcttgccgcc caccatgtgc gtcgcctggc
    12181 cgaaatagag ctcggccgct tccatcagcg tctccgacag cgtcgggtgg gggtggatcg
    12241 tgtcggccac gtcgcgcgcc agcgccgcca tttcgatcgc cagcgccccc tcggcgatca
    12301 tgtcgcccgc gttcggaccg accatcccga cgcccaggac gcgctcgctc tccggctcga
    12361 tcaccagctt ggtcagcccc tcggtgcggc ccagcgtggc ggcccggccc gacgccgccc
    12421 aggggaagcg cgcgaccttc accgcgatgc cgcgcgccgc cgcttcgttc tcggtcagac
    12481 cggcccacgc cagctccgga tcggtgaaga ccaccgcggg gatcgccgcc acctcgaacg
    12541 ccgaccggtg gccggcgatc acttcggccg ccaccttcgc ctcgcgcgac gccttgtgcg
    12601 cgagcatcgg ctcgcccgcc acgtcgccga tggcgaagat ccgttcgtcg ccggtccgct
    12661 gctgacggtc aaccttgacg aaacccttct cggtcaactg cacgccggtc cgctccaggc
    12721 cgagcccttc gctattgggg cgccggccca ccgcgatcag cacgcggtcg aacagcatct
    12781 ccggcgccgc cccctcgccg ctgagcttga ccttgatccc ctgcttcgtc ggctcgagtc
    12841 cctcgacccg ggttttcaga tgaatcgcct tgaagctctc ttccagccgc ttgtgcaacg
    12901 gcttgaccag atcgcggtcg gcgcccggca gcagcccgtc ggtcagttcc acgacggtga
    12961 cctccgaccc cagcgcctgg tagatcgtcc ccatctccaa gccgatgtag cccccgccga
    13021 tgacgagcag cttcttcggg acgtcgggca gattgagcgc gccggtcgag ttcatgatcc
    13081 gctcgtcgtc gatctggaac ggaccgggga tcgccgggcg cgacccgctg gcgaggatgc
    13141 agtgctcgaa gctcaagcgc tcggggacgc cctcggcccc ttcgagcggg gccagcgcca
    13201 gcgtcgttga atccagaaac gaggcgcgcg ccgcgaggta gcgcaccttg cgcgccccgg
    13261 cgatctggcg cacgcccccc gccagccgct tgaccacctg gctcttccac gcgcgcagcg
    13321 cgtccaggtc gatcttcggt ttgccgaaac tgaggccgca gacctctgcc tcgcgcgatt
    13381 cgttgatcag acgggcaacg tgcagcagcg ccttggacgg gatgcagccc cggtgcaggc
    13441 agacgccgcc cgggtcgggc tcggcattga ccagcgtcac ctccagcccc agcgtggccg
    13501 cctggaatgc cgctgcgtat ccccccggcc ccgcgccaat caccacgacc tgtgccttgt
    13561 cgcccgccat catccatcct tccagcgtcg gagatcgctc ctggttttca gtctaaaatc
    13621 gtccctcgtc ctcgtgatcg cccccaaagg cctacccaaa actcggctct cggctctcgt
    13681 ccctcgtcct cggctctcga taaccggcca atctgtccct tcgttgtgaa gcgcacgttt
    13741 gatctgttct gccgtccctg cgggacgggc cggctgactg atccggccag ccgtggaccg
    13801 tacgattcgg ttgttcaccc tcccattcaa gtggttcgtt gtgaaccctc gctgcatgag
    13861 caacccgtga tccgtcgtta gcgccccgtg tcccttttcg ctcctcccct tcaaatctca
    13921 aacagcatgt cgaacgggct ggtcagcagc tcgcacatct cgcggacgaa ccgcgccgct
    13981 tccgccccgt cgatcacccg gtgatcatac cccaggcaga ccggcaggag gaggcgcacc
    14041 cggccgttgc cctcccggtc gaagacctgc tcgtaagcgg cgcgcgagac gcccaggatc
    14101 gccacctcgg gcaggttgat gatcggcgtg aagcccaccc cgccgatccc ccccagattg
    14161 gagaccgtga acgtcccgcc cgacatctcc tcggggctga gcttgccctc gcgcgccctg
    14221 ccggccagcg cgtgcagatc ggccaccagc tcgctgatgc tcttgcgatc gacgtcgcgc
    14281 acgaccggca cgagcaggcc ccgttccgtg tcggccgcca cgccgatatg gcagtaatgc
    14341 ttcaagatca gctggttgtt cgccgcgtcg aggctggcgt tgaacttcgg gtgccggcgc
    14401 agcagcttgc ccagcacctt gagcagcacc gccgtgacgg tcagcttgcg ggccggatcg
    14461 tcgatggcgg cgttgcgccg ccggcgcagc tcctccagct cggtgatgtc ggctttgtcg
    14521 tgatgatgga cgtgggggat cgtctgccag gagaggctga gccgttcgct gatgacgcgg
    14581 cggatgttgt tcagcggctc ggcctcgatc tcgccccagc ggctgaaatc gggcagatcg
    14641 gccgatacac tgacggcggg gcgcgccgcc gcacgggccg gtcgcggcgc gccgccggcc
    14701 tggcgccgcg cgtgcgcctt cacgtcgtcc tcgctgatcc ggccgccggg gccggtcccc
    14761 tccacctcgc tgatgtcgac cccgatctcg cgcgccagcc ggcgcaccga gggagatgcc
    14821 ggaaccgtct ggcccggcgc cggcggcgcc ttgcgcgcgg cgggcgtcag ttgctccacc
    14881 cgcgcctcgc ggcgcggcac cgccttgcgc tcgcgcgaat cctcctccgg ctccggcgcc
    14941 tcctccgcgg cggcggtctc gcgctgttcc tccgcgcggc gcgcttcctg tttccgcgaa
    15001 accggcggct cttccttcgg cgccttttcc gcgcgggccg gccgcgactc gccggcttcg
    15061 ccctcttccg cgatcgtcat cagggtcgcg ccgaccggca ccgattcgcc ctctttgacc
    15121 ttgatcttcc caaccttgcc ggtgaccgtc gaggggactt cgatcgtcgc cttgtcggtt
    15181 tcgatttcga cgagcgtctg ttccttcttg accgtgtccc cctcgttcac gaggacgcgg
    15241 atgacagtgg ctttttcaat gttctcgccg acttcaggca gcttgaactc ggtggccatg
    15301 cgaaactctc ccctcctgat ttgccgccgc gcgcgcgcag cttacgagac catcggattc
    15361 ggtttggcgg gatcgatgcc caggtccttg atcgcctggg acaacacgcc cggttccagc
    15421 tcgccttccg cgacgagttc ggtcagcgcc gccagcgcca catgccggct gtcgacctcg
    15481 aagaagtcgc gcagcgccgc gcgcgattca ctgcggccga acccgtcggt ccccagcgga
    15541 tggaatcggc cggggatgaa ccgcccgatc gactcgggca gcgccttcac gtagtccgac
    15601 accgccaccg tcacgttcgg ccccttctcc agcagttgcg tcacatacgg cttgcgcggt
    15661 ttcttctccg gattcaaccg gttccagcgc acgcactgct cggcgtcgta atacagctcc
    15721 ttgtagctcg tgacgctgaa gacgcgggcc gccacgccgt attgctccgc caggagctgc
    15781 tgcgccttga gcgcctcgtt gatcagcgac ccgcccccca tgaggttgat cttgtgcttg
    15841 agcttcttct tcggcgcctc gcgcaacaga tacatcccct tcaggatgcc ctcgcgcgcc
    15901 ccctcgggca tcggcaactg cgagtagttc tcgttgtaca gcgtgatgta gtaaaagata
    15961 tcctcctgct ccacatacat ccgccggatg ccgtcctcga tgatcaccgc gatttcgaac
    16021 gcgaacgccg gatcataggc cttcaggttg ggcagcggat aggccagcag ctgactgttg
    16081 ccgtcctcat gctgcaagcc ctcgccgttg agcgtcgtgc gcccggccgt gccgcccagt
    16141 atgaacccgc ggcagcggca gtcgccggcg gcccagatca gatcgccgat gcgctgaaac
    16201 ccgaacatcg aatagaagat gaagaacggc agcatgttga ccccgtgagt cgcatacgcc
    16261 gtccccgccg cgatgaacga cgacatcgag ccggcctcgg tgatcccttc ctccaggatc
    16321 tgaccgtcgg tcgcctcctt gtagtacagc agactgcttg cgtcgaccgg ctcatacagc
    16381 tgacccacat gggagtaaat gccgaactgg cggaagagcg cctccatgcc gaaggtgcgc
    16441 gcttcgtccg ggacgatcgg cacgatccgc ttgccgaatc cttcctgccg catcaacttg
    16501 gccagcagcc ggacgaaggc catcgtcgtc gacacctcgc gctcgtcgcc gctgtggaat
    16561 tcggtgaaca gttccggatc gagcggcgga aacgcctcgg tctgcggatg gcgttgcggc
    16621 tggtggccgc caagcgcctc gcgccgctcg cgcagatact gcatttcggg gctgtcgtcc
    16681 ggcggtttgt aaaacggcgc cttggccact tcctcgtcgg agatcgggat gccgaaccgc
    16741 gtccggaact cgcgcagctc gtcctcgttg agcttcttct gctggtgcgt gatgttgcgc
    16801 ccctcgcccg cttcgcccag gccgtagccc ttgaccgtct gcgccaggat caccgtcggg
    16861 gcgcccttgt gctcgaccgc ggccttgtac gccgcgtaaa ccttcaccgg atcgtgcccc
    16921 ccgcggcgca gcttgcgcag cgattcatcc gtgtggttct cgaccagctt gagcagctcg
    16981 ggatacttgc cgaagaactt ttcgcgcacg taggcgccgt cctcgaccac cagcttctgc
    17041 gcctcgccgt cgatcagttc gttcatccgc tgcaccagca ggccgctctc gtcgcgctcg
    17101 agcaattcgt cccagtcgct cccccagatg accttgatca cgttccagcc ggcgccgcgg
    17161 aacgccgctt ccagctcctg gatgatcttg ccgttgccgc gcaccgggcc atcgagccgc
    17221 tgcaggttgc agttgatcac gaagatcagg ttgtcgagct tctcgcgcgc ggccagcgta
    17281 atcgcgccga gcgattccgg ctcgtcgcac tcgccatcgc ccatgaagca ccagaccttg
    17341 ctctcgctcg tgttcttgat gccgcggtcc tccaggtagc ggttgaaccg cgcctggtag
    17401 atcgccatga tcggcccgag gcccatcgag acggtcggaa actgccagaa gttcggcatc
    17461 agccacgggt ggggatacga cgaaagcccg cccccctcgg ccagttcgcg ccggaaattc
    17521 atcaggtgct cttcgctgat ccgcccctca aggaacgcgc gggcgtagat ccccggcgag
    17581 gcgtgcccct ggaagtagat ctggtcgccg ctgaaatcgt cgcgccgcgc gcggaagaaa
    17641 tggttgaatc cgatctcgta gagcgtggcc gacgaggcat acgtcgagat atgcccgcca
    17701 atcccggcgc tggccttgtt cgcgcgcacg accatcgcca tcgcgttcca gcggatgatc
    17761 gacttgatcc gccgctcgcg ctcgcggctg cccggaaacg gcggctgctg gctcaccggg
    17821 atcgtgttca cgtagggcgt gttggccgaa aacggcagca ccacgccgtt gctgtaggca
    17881 aaatcgcgca gcttgccgat cagttcgcgc acccgctcgg gaccctggcg caccagcaca
    17941 tcctcgagcg agtccatcca ttcctgatct tcgatggcct tgagcgtttc ctgctcgtcc
    18001 agctcgtgct cgttctcggg cgcatattca tacatgggct tggacttctt tcgaccttga
    18061 aaggttgtcg gcatctccac tcactctcaa caaacgtcgc tcgacttctg gtcccgggct
    18121 ttcgaaaccc cctttggctc cggccacctc aatccgcgcc catccgtgtc cgcgcgcgtt
    18181 tttggccccc gttccgcgcg ggccgacgcg gcggcatccc gcggcgctgg tcgcatccgc
    18241 cggcgattat ggcaaactcc cgccggtctt tccaccccgc gaagccgcac ggccccgccc
    18301 ggccggggtc cgaatcagat ctcttgctcg cttacacgac cccggaagcc ccattttagg
    18361 ccccctccga tgaaaaaaca agcatcgagc aacctgaagc agcagacgtg gatccctgct
    18421 gttcgtgatc gggatcaccg cccattcttc gacccctgat cccccacctg gcccgtccca
    18481 tcatcaccat ggtcgaagct gcgacgccga tgctatcctg accttggaag ggagggaggt
    18541 tccatgcacg aaatcattgc gattcagcca ctgggcgact tcaagttcga tttggagtct
    18601 gcggatggga cccgcggaac agtggctctt tccaacctgc gcggcaaagg ggttttcaag
    18661 ctgtgggacg aacccggcgc ttttgaagcg gtcaagatcg ggtcgtcggg tgaactcgtt
    18721 tggcacgacc gcattgatct ctgccccggt gccctttgcc ttcgggtcac cggcaaattg
    18781 cctgaagaag gctttcctgg gaaaatcccg ctcatgcctg aactcagctg ctgctttggc
    18841 atcatcatca aaatgttttt cgatgaccat gccccgccgc acttccatgc agaatagggc
    18901 gaatacaagg cgttgatcga tatctgctgg gatcgcgtct gtagatacga agacttgcag
    18961 agaatcactc cgcttgtata aagcgactcg ttgaattgga agagactcag ccccaccatg
    19021 caccacgccg cgatgctctg gaccggaggg aaagactgcg cgcttgcgct cgacgaggca
    19081 atccggaccg gcatcgacat ccgctgcctg gcgacgttcg ccccgccgcg acccgacttc
    19141 ctcgcccatc cgctcgcgct gatgcagctt caggcgcggg cgatggggct gccgcaccac
    19201 atcgtcgccg tcaccgcgcc gttcgaccag ggctatgaaa ccggcctcgc ccgcctgcgc
    19261 gacgagttcg gcatcgacgg cgtcgtcacc ggcgacatct ccgaggtcga cggctgcccc
    19321 aactggatcc gcgagcgctg ccgccccgtc ggtctcgacg tcctgacgcc gctctggg
//
LOCUS       DAOPCO010000045        18682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3066_length_18682_cov_5.207280, whole genome shotgun sequence.
ACCESSION   DAOPCO010000045 DAOPCO010000000
VERSION     DAOPCO010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 18682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18682
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_18682_cov_5.207280"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..1289)
                     /locus_tag="PLS90_05365"
     CDS             complement(249..1289)
                     /locus_tag="PLS90_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HPK01865.1"
                     /translation="MAKKKISKTAGGATSRNSKKAKAPAVRAKSPARKAASKPSAGAE
                     RRAAKPAAKKSLAGKGAVRKAAPKKAVKAKPAAGGKSVAKKRPAARPKPAAPKPVKKQ
                     SAAKKKVAVKKVTAKKAAPKATPKSRPAAKATRPAVKRPSAAPAAAEKKPADGAQARR
                     DAVRKAAAEQAYTVARKARERAREEKDRLIPDLDRPTGMYNGIILAENPKPFPQRTPY
                     SKKELEKLRDALREERARLISQLESIHRLNMETLDLAKEHPGFSVHMAEHATDLQTAE
                     ANLGVRTIEEERLTLVEAALDRLENNVNHYGLCLACGAKIGIQRLIARPHAHLCLDCR
                     QRYEKMRSRRGY"
     gene            complement(1289..4087)
                     /gene="ileS"
                     /locus_tag="PLS90_05370"
     CDS             complement(1289..4087)
                     /gene="ileS"
                     /locus_tag="PLS90_05370"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPK01866.1"
                     /translation="MTQQEKTDYKETLNLPRTEFPMQARLPQREPVRFAQWEAEGLSA
                     RIVAQHANDPVFCLHDGPPYANGAIHIGHALNKILKDVLIRYKAMSGHKAYYRPGFDC
                     HGLPIEQKVLQKLGGKARKLSPVEIRAECRRYAQKWVDTQREEFKRLGVGGDWDNAYL
                     TMSHETSLGILRAFRDLVARGVVRRGMRPNYWDPVFRTVLAEAEIEYATHVSDSIYVK
                     FPLTEGEAVAGLAGLANVSLVIWTTTPWTLPANLAVCLHPDYEYVVLRSGAEHFVVAR
                     ELAESFRQACGLGEAPVVARVNPKDLERKTCRHPIFDDRTSLIILGTHVTLDAGTGCV
                     HTAPGHGLDDFHVGQQYGLDVFVPVDEAGQFTADFPAMAGVGVFDANPRIIEMLEQKG
                     LLLHAGKIEHQYPHSWRSHKPIIFRATEQWFIDLEVGRIRQLALQAIDQIRWIPEWGR
                     DRIYNMVESRPDWCISRQRAWGVPIPAIRSKATGAVVLDVRILDTVLEVMARKGEDAW
                     WTDPLCDFWPEGFICQETGETSPDQFEKLYDILDVWFDSGATSIAVCEADPNLTMPAD
                     LYLEGGDQHRGWFQTSLLLRIAARGEAPFRAAMTHGWVLDGQGEAMSKSKGNVIAPQE
                     IVQTMGADGLRLWVVSEDYRADLTISQDILRRVADAYRRIRNTFKFLIGNLSDFDPER
                     DALPVAELEEFDRWIISALNRLIEKVRHAYERYEFHRIYHLIHNFCSVQLSALYLDVI
                     KDRLYCSAPGDMTRRSAQTACHHLLHNLVRMVAPILVFTADEVWGFARLGPESNVHLA
                     SFPTPNESWAAPDLEEKWERLLELRSEVSRVLEEARREKRIGHSLEAAVEISSADEAE
                     VAFLRKNIEILKDLFIVSAVQVQHLQTLESPGRSVTAAAAAGRKCQRCWMIDPAVGQD
                     SAHPGLCGRCLDVIKRIG"
     gene            complement(4150..5010)
                     /locus_tag="PLS90_05375"
     CDS             complement(4150..5010)
                     /locus_tag="PLS90_05375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPK01867.1"
                     /translation="MMEAFDLEQYKQSIAEATAAIRERHAEAPRFGIICGTGMGGLAD
                     RVEEKTVIPYQQIPHFPLSTVESHSGNLMLGRLSAQPVVVMQGRFHRYEGYSLREVTF
                     PVRVMRALGCEALIVMNAVGSMNPLIRRGSIVLVDDHVNFMGDNPLIGPNDEAMGPRF
                     PDMSEPYDPEMRAILRQVALENQIRLEEGVMVAVTGPNLETRAEYRMFAQLGIDIITM
                     STVPEVIVARHAGMRVVALSTVTDECLPDALRPVAIEEIIATARSAEPRLERLILGLL
                     ARIGARGISG"
     gene            complement(5036..5344)
                     /locus_tag="PLS90_05380"
     CDS             complement(5036..5344)
                     /locus_tag="PLS90_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HPK01868.1"
                     /translation="MEWPCLREHPEGCLLQVRLLPNASRSALEGLREGRLTARVQAPP
                     VEGKANQALCRVVADALGLRPADVILLRGERSRQKDLLLRGMNLDLARARLSAVARDA
                     "
     gene            5731..6324
                     /locus_tag="PLS90_05385"
     CDS             5731..6324
                     /locus_tag="PLS90_05385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPK01869.1"
                     /translation="MMTKILGVVGSLRSRSHTRKLVALALEEAAAIGAETRLLNLEET
                     PLPMFNPERRGADPVEQQVRELVVWADAFILGTPDYHGSMSGAMKNFLDFFWKEFTGK
                     LFAYIVASHEKGLTVQDQLRTAVRQCYGWSLPYGIGFNGDAVFDGNDELIDEVVAERV
                     RMLAHDLTVYGGLIHGQFLREIQNEPRAPGFARNFRP"
     gene            complement(6406..7623)
                     /gene="alr"
                     /locus_tag="PLS90_05390"
     CDS             complement(6406..7623)
                     /gene="alr"
                     /locus_tag="PLS90_05390"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPK01870.1"
                     /translation="MKPESEQGDKLTSRRANASEGMEPFTARLREETARWARTWCLID
                     LPALRRNLRRVHERLAPGRRLIVSAKKDGYGHGLLAVAGEVDQLRQWSGRDITLGLMT
                     IEEPIALREAGYDGDLLLFATLAGEALGEAIRLDAALTITDLEDARAADEAAGRLGKL
                     ARVHLKVDTGMGRLGRRVEELPSQIARIRALPNLSLESVWTHLANPKGDPESARRQIE
                     LLEGLRRMTGLLDLPAHLGGSDAVALIDGQAGGDYDFRAGISIYGDSSLIAGLEPVMT
                     FVSHVIYRKHVPPDWPISYGQTHRTVRETELALIGAGYGNGVLRTLGNRGHVLLRGRR
                     CPILGRICMDQFMVDVTECCDVQVGDEAVLFGRQGSAVLTAAEAARAAGTVSYELLCL
                     AGQINPRRYRHAD"
     gene            7622..8971
                     /locus_tag="PLS90_05395"
     CDS             7622..8971
                     /locus_tag="PLS90_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017292002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPK01871.1"
                     /translation="MTFTASCRPAESVQGTSMLTQTNGKINKLDAADRSVHDWYRFVL
                     SFPPHLVRGYLEKFGVDGSGLVLDPFCGTGTTIVECKKQGIASIGIEANPMAHFASTV
                     KTSWSINPGKLITWANEIADSASGEFERQGIADVELFDSNDGRERYEMRTLPAEQMKL
                     LLKNSISPRPLHKTLILRDLIIGSVDRSCAAYGRLALAKSLVEDIGNLHFGPEVGVGV
                     IKEDAPVIQSWLANTIGMARDLETVRPRGKTEATVLHGDARDLSHLVRDRKFSVVFTS
                     PPYPNEKDYTRTTRLESVLLGFINDKSELRRMKQHLIRSNTRNIYQQDRDEQWVKGNK
                     RVQDLADKIEARRIELGKTSGFERLYARVTRSYFGGMYRHLADLRDFLKPGAWLGYVV
                     GDQASYLRIMIRTGQILGEIAEELGYRVESIDLFRTRLATATREQLNEEVLVLRWPG"
     gene            8959..9777
                     /locus_tag="PLS90_05400"
     CDS             8959..9777
                     /locus_tag="PLS90_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013535281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPK01872.1"
                     /translation="MARMKPETPVSRYSELIEAVFLEHYSPGVDQFTFDRDELVQHAR
                     RRRIRLPKNLGDVIYSFRYRKNLPEKIQATAPEGMSWLIRSAGVGRYRFVLTKGEIRF
                     KPNALMSVTKIPDSTPGLISRYALNDEQALLAIIRYNRLLDIFTRVACYSLQSHLRSS
                     LPAIGQVETDEIYVGVDRQGAHYVFPVQAKGQKDTLGTLQIEHDIHLCRHKFPGLICR
                     PIAAQFARGRTVVLFEFDDNAQELRLVRECHYRLVNPDEISREEMERYRTLSDE"
     gene            9780..11210
                     /locus_tag="PLS90_05405"
     CDS             9780..11210
                     /locus_tag="PLS90_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPK01873.1"
                     /translation="MYAQAVTIPAAPSEPSGLPEPQTRGYFFNLVRSISPTSLELELR
                     RVAEAGFNLVVFPVYSNGWTLFQSATAREYGLPSINPRLSKWDAVEQVIRLADRFGLT
                     VWGFARPYNFHPRYSIAEHRLLRKHPDWRLRAHPQHLTARTRRAESLHPCPLNPDYRR
                     FVGSLLAELAAGYNIPGIVINYTAVGIEGGPIKSSPFCFCASCRERFRETHGVELAAE
                     ALHGDLELVRAWQRDQMHLHLRYLRHRLLKMRHSLRLVARVRPQWREGAFDSQLLTPG
                     VSLLDWSTLAREGALETLVIDHDNERLGPLFSTRLAADYAYLGDQVLFLPMLSVHDLD
                     DLSIPMAVLRRYPCTGFIGEFQRSFTDGDTQFLARQVFGNPAILPSDRPLQTAAYFLN
                     LVHQTHQDQPELRELTRDILRLISRQSPHPHDFELLRVMEQNLRGLEQAIRRGALGFG
                     SIPERTLIHLGLARRFVRIALMDVPT"
     gene            11210..13771
                     /locus_tag="PLS90_05410"
     CDS             11210..13771
                     /locus_tag="PLS90_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01874.1"
                     /translation="MRPAFNRHLGQRPLVALLLCYLLLALAILHGGGPRQAGSLRGTG
                     IREAPDDEAVLPGILRAPLMLTRTVPAGSDTLGHDYPAWLWGWSQIRRTKQIPLWNPC
                     LFAGMPFLASQMFMPFYPFNWLGAVLPFPLAFNIQYPLHLVLAALVMAFTVRRRRMTW
                     WTAFLVGIAWGFGSHLATLAGPGHLQKLQALAWLPLVAWGGACLGRGAGRAGALALGA
                     GLALQITAGHLQIVYLSLLVALLEALAALAVAFLRPRDPAPAALSRLQRRRALARSLG
                     WTALGLAGALGLSAVVLIPTLEFARISNRQGSLSWDEATRGSLPPEEKFEFIFPRLLG
                     DSMPAGRGPYLGRYGETDSGATPERIVSDYVGAGVLLFSLLALALPGRRRVALAWLLL
                     AAGALILSIGRYYAGLYRFLLDWLPGLAHFRSPSTSMAVLSYALAMAAAQGVECWLAE
                     GRNRAHASRGPAGWIGHGMILLAAGAAALALVAAFWLRDARQADLPRAVAQAAAALHT
                     FRFLALVAGVAGLWRMVRRGERAARPWIAPALLGVLALVWAWDLVLNVRPFWTVEAAA
                     PYERHIQHHWAERVWRSEARPVRFLVPGRELQNWAMTYTGTGPGPIISSISGYHPVVL
                     DVYESFIERLGFLHPNLLRLLAVNYLVWPDDPDLPFPEGYLPVSRMSGEVLLHNPQYP
                     YVRPIRHFREVASLDALLDRLEDPRFQPYESTLVLEEGKHPKSLLKGTPEATHLSAEA
                     TSIAPGDVTIQIRTRNWGAVALSELAVPGWRIQPVDPAAGVRIEPFIADGLLLGFAFP
                     AGRHTVRCTYDPVSQRLGLYITLVTLAALACAAGRKLAERRRAGTQPATGEAWTS"
     gene            complement(13738..15117)
                     /gene="rimO"
                     /locus_tag="PLS90_05415"
     CDS             complement(13738..15117)
                     /gene="rimO"
                     /locus_tag="PLS90_05415"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPK01875.1"
                     /translation="MPLSPVSTRPLHVGIVTLGCDKNTVDNEYLAGLLEAGGCRLVVD
                     GEAADGPLDAVVVTTCGFIDRARQQSIDTIVELAERKRATGNPRRLFVAGCLSQRHGA
                     ELMEEIPEIDGLVGVGQFRQLANLILNADRAEARTREVAETPTVDIYQLMHRRRLQHT
                     PHAFLKISDGCNHACTFCSIPAMKGKLRSVPREILLAEAERLLADGVREINLVAQDLS
                     MYGMDRWRDVRLPDLLRDLCALPGEFWIRCLYFYPGNVTDDFLEVMAAEPKIVPYLDM
                     PLQHLHPETLRRMRRPFHEVKTFELVERLRAAVPGLTLRTTMIVGFPGETRAEHEFML
                     EGIRRLRFERLGAFQYSDEEGTPSATMRPRVRRGEKERRWHAVMAAQAEIAAEHSRAR
                     VGRRERVLVESHEPHAGCWIARGAAEAPEIDARVFVWSATPLTPGQFIDVEINRADGY
                     DVHASPVAG"
     gene            15321..17525
                     /locus_tag="PLS90_05420"
     CDS             15321..17525
                     /locus_tag="PLS90_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01876.1"
                     /translation="MPHRSARLDALMLALVLLFAVWLLGRMIAALHLGAVAAAFHNLL
                     SLFTRPWHPPVDAIAGFAVGMILFAVLVGLGYAILTSTRSGRRMLPLLSERVLLAPLV
                     GAVPVSLIILLWGLAGWLGRAPLIALLAGFGLLSLRGWLRLGPALWARRALLVRFIRG
                     QSPLALALGLLVALWIVPYTLSPAVESDELRYHLSAPAQWLARGRIEYLPWQAFSNFP
                     FLAEMLYLLAMGVSGPPYQWLAEPARLLHLTMLPVNMGLIALLALRLRPRAARRRAPD
                     TSSAWWFLFLPSAPILAAWAFVDLFMSAYFLGFVYLGLVSLRRPRRAVGMLLGLLAAG
                     AVGTKYSMIPLVSVLGLFWGGLLIWRSGRRLAARRLGGAALTGFLFGAPWLIRNLVWT
                     GNPFYPLAWSWFGGGEWSRENMAFYLEKAGAKGMHLWPDRWWGPLADWLLTPWNVTFH
                     IDRYEQHYLGPLPLMALALVLGWGLAAAIPKSPRPRPPGRRPARLWLLAGIVISWAAW
                     FATYQSARMLLPTIGLLFAVAGAAWNGWIGAQPRRSLRMAARALWGVAAVYCVMFLVA
                     VLGQTGRGSAGKLDAIAVGLGFERPEVYLARRLNYASAARRLGQLAAPGDKALLIGEH
                     RTLHFPLPVVASDWFDTPQPLPWLRRLPADDDVFDALWQVGVRFVLLNRAELALYYET
                     DFLPRLKPGEAERLGDPFIPHGRGAGFLHSPRLTPLGERGGVALYRLSPPAS"
     gene            complement(17590..>18682)
                     /locus_tag="PLS90_05425"
     CDS             complement(17590..>18682)
                     /locus_tag="PLS90_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01877.1"
                     /translation="LDAAGGDERTLVRQTPASGGVHLHTTFIGTIGDVDGDGENELLT
                     TTAEQGRAVVAVDAAGQEKLRLTAGDDPGEMVLVATGKLAGKPGNWITIRWQEGEKTW
                     GVPWTVATYDATDGRLLWERTTYGLYGGKPSTFGIHVPTSVADYNGDGGDDLFVASEN
                     FFGIIDATTGEDLLAAKNILDQRADKIWPAYSKLLLVEMDGQAPLELVHSHAFAATYV
                     ATWESEPLWSVKLTRDTTAAHYPGVVDLDGNGVKELVTSQVDGLLIAYNHVAAGEAAS
                     ERWRFSLPGRVSDLAVVNLDSDGQEELLAGAADGKLYALKEADGQCQVLWTVDLGSPV
                     GSPIVAELGEAGSASILVPTQDGYLYCLR"
BASE COUNT         2854 a         6471 c         5992 g         3365 t
ORIGIN      
        1 ccgctcgccg ccccgttcga ccggccaggc ggggtcgaac ggggcggcga gcgggccgcg
       61 cgggcgctca agcggttggg ggacggcggt ttcccggggc ggcgtcatgc aatcatgcta
      121 gccgaagccg ggcagcgact ccagcacggg ttcgcgcggc cggcgacaaa tgcgaacagg
      181 gccgcgggac ctgagtaaat gtctccttat gtctcttcct ttatgtccct tgcgtccctt
      241 atgtctgttc agtacccgcg ccgtgaacgc atcttctcgt agcgctgccg gcagtccagg
      301 cacaggtgcg cgtgcgggcg ggcgatcagc cgctggatcc cgatcttggc gccgcaggcc
      361 agacacagcc cgtagtgatt gacgttgttc tcgagccgat ccagcgcggc ctccacaagc
      421 gtcaggcgct cctcttcaat cgtgcggacg cccagattgg cctcggcggt ctgcagatcg
      481 gtcgcgtgct cggccatgtg gaccgagaac ccggggtgtt ccttggccag atcgagcgtt
      541 tccatgttga gccggtggat cgattcgagc tgactgatca gtctcgcccg ttcctcgcgc
      601 aacgcatcgc gcagtttctc cagttccttc ttgctgtagg gggtgcgctg ggggaacggc
      661 ttggggttct cggcaaggat gatcccgtta tacatcccgg tggggcgatc cagatcggga
      721 atcaggcgat ctttctcctc gcgcgcacgt tcgcgagcct tgcgcgcgac ggtatacgcc
      781 tgttcggcgg cggccttgcg caccgcgtcg cggcgcgcct gggccccatc ggccggcttc
      841 ttctcggcgg cggccggcgc cgccgatggg cgcttgacgg ccgggcgggt agccttggcg
      901 gccggacggg acttgggcgt cgccttgggg gcggccttct tcgccgtaac cttcttcacc
      961 gcaacctttt tcttcgccgc ggactgcttc ttcaccggct tgggcgcggc gggtttcggc
     1021 cgggcggccg gacgcttctt cgcaaccgac ttgccgccgg cggccggttt cgccttgacc
     1081 gccttcttcg gcgcggcctt gcgcaccgcg cccttgccgg ccagcgactt cttcgcggcc
     1141 ggtttggccg cgcggcgctc ggccccggcc gacggtttgc tggcggcctt gcgcgccggg
     1201 ctcttcgccc ggacggccgg cgccttggct ttcttcgagt tccggctggt agcgccgcca
     1261 gcggttttgc tgatcttctt cttggccatt atccgatcct tttaatgaca tcaaggcagc
     1321 gaccgcatag gcccggatgg gcgctatcct gacccacggc ggggtcgatc atccagcaac
     1381 gttgacactt ccggccggcg gcggcagcgg ccgtcacgct ccgccccggc gactccagcg
     1441 tttgcaaatg ctggacctga actgcggaaa cgataaacaa atctttaaga atctcaatgt
     1501 ttttgcgcag aaaggcgacc tcggcctcat ccgccgacga tatctcgacg gcggcctcca
     1561 gactgtggcc gatccgcttt tcgcgccggg cctcctcgag cacgcggctg acctccgagc
     1621 gcagctccag cagccgctcc catttctcct ccaggtcggg cgccgcccag gactcgttcg
     1681 gcgtggggaa cgacgccaga tgaacattgg attcgggccc caggcgggcg aaaccccaca
     1741 cctcgtcggc ggtgaagacc aggatcggcg ccaccatccg aaccaggtta tgcagcagat
     1801 ggtggcaggc ggtctgggcc gagcggcggg tcatgtcgcc cggcgccgag cagtagagcc
     1861 ggtccttgat cacgtccaga tacaatgcgc tgagctggac cgaacagaag ttgtggatca
     1921 gatgatagat gcggtggaat tcgtagcgct cgtaagcgtg gcggaccttc tcgatcagcc
     1981 gattgagggc ggagatgatc cagcgatcga attcctcgag ctcggccacg ggcagcgcat
     2041 cgcgctccgg gtcgaagtcg ctcaggttgc cgatcagaaa cttgaacgtg ttgcggatcc
     2101 ggcgataggc gtcggcaacc cggcgcagga tgtcctgcga tatcgtcagg tcggcgcggt
     2161 aatcctcgct gacgacccac agccgcaggc cgtcggcgcc catcgtctgg acgatctcct
     2221 gcggcgcgat cacattgccc ttggacttgc tcatcgcctc gccctggccg tccagaaccc
     2281 agccatgcgt catggcggcg cggaacgggg cctcgccgcg ggcggcgatg cgcagcagca
     2341 gcgaggtctg gaaccagccg cggtgctggt cccccccctc caggtagagg tcggccggca
     2401 tggtcaggtt ggggtcggcc tcgcagacgg cgatcgatgt cgcgcccgaa tcgaaccaga
     2461 catccaggat gtcgtacagc ttctcgaact ggtcggggga ggtttcgccg gtttcctggc
     2521 agatgaaccc ttcgggccag aagtcgcaca gcggatcggt ccaccaggcg tcctcgccct
     2581 tgcgggccat gacttcgaga acggtatcca gaatgcggac atccaggacg acggcgccgg
     2641 tcgccttgga acggatcgcc gggatcggca cgccccaggc gcgctggcgc gagatgcacc
     2701 agtcgggccg cgactcgacc atattataga tgcgatcgcg cccccattcg gggatccagc
     2761 ggatctgatc gatcgcctgg agggcgagtt gccggatccg gccgacctcc aggtcgatga
     2821 accattgttc ggtggcgcgg aagatgatcg gcttgtggct gcgccaggag tggggatact
     2881 ggtgctcgat cttgccggcg tgcagcagca ggcccttctg ctccagcatc tcgatgatgc
     2941 ggggattggc gtcgaacacg ccgacgcccg ccatcgcggg aaaatcggcc gtgaactggc
     3001 ccgcctcgtc gaccggcacg aaaacgtcca gaccgtattg ctggccgaca tggaagtcgt
     3061 cgagcccgtg gcccggagcg gtgtgaacgc atccggtgcc ggcatccagc gtcacgtggg
     3121 ttcccagaat gatcaggctc gtccggtcgt cgaagatcgg gtggcggcag gtcttgcgtt
     3181 ccagatcctt cggattgacc cgggcgacga cgggagcctc gcccaagccg caggcctggc
     3241 ggaaggactc ggccagttcg cgcgcgacga caaagtgctc cgcgcccgac cggagcacga
     3301 catactcata atccggatgc aggcagacgg cgagattggc gggcagcgtc cacggcgtgg
     3361 tcgtccagat cacgagcgaa acgttcgcca aacccgccag gccggcgacg gcctcgccct
     3421 ccgtcagcgg aaacttcacg tagatcgagt cggaaacgtg cgtcgcgtat tcgatttcgg
     3481 cttcggcgag caccgttcga aaaaccgggt cccagtaatt ggggcgcatg ccgcgccgga
     3541 cgacgccgcg cgcgaccaga tcgcggaacg cccgcaggat gcccaggctc gtctcgtgcg
     3601 acatcgtcag gtaggcattg tcccagtcgc cgccgacgcc gagccgcttg aactcctcgc
     3661 gctgggtatc gacccatttc tgggcgtaac gccggcattc ggcgcggatt tcgaccggcg
     3721 acagcttgcg cgccttgccc cccagcttct gcaagacctt ctgttcgatc ggcaggccgt
     3781 ggcaatcgaa cccggggcga tagtacgcct tgtggccgct catcgccttg tagcggatga
     3841 gcacgtcctt cagaatcttg ttcagggcat gaccgatgtg aatcgcgccg ttggcatagg
     3901 gcgggccatc gtgcagacag aagacggggt cgttggcgtg ctgcgcgacg atccgggccg
     3961 aaagcccctc ggcttcccac tgggcgaaac ggaccggttc gcgctgcggg agccgggcct
     4021 gcatcggaaa ttccgtccgc ggcagattca aggtctcttt atagtcggtc ttttcctgtt
     4081 gcgtcatggc ctggcggatc aacctcgata agcctgggat ctgtcggcgc cggtcgccgc
     4141 acggccattc tagccggaaa tcccgcgcgc gccaatccgg gccagcaggc ccaggatcag
     4201 gcgctccagg cgcggttcgg ccgaacgggc ggtggcgatg atctcctcga tggcgaccgg
     4261 ccgcagcgcg tcgggaaggc attcgtcggt cacggtcgat agcgccacga cgcgcatccc
     4321 ggcgtgccgg gcgacgatca cctccggcac cgtggacatc gtgatgatgt cgatgcccag
     4381 ctgcgcgaac atccggtatt ccgcccgggt ctccaagttg gggccggtca cggcgaccat
     4441 gaccccctcc tcgagccgga tctggttttc gagcgccacc tgccgcagga tcgcgcgcat
     4501 ttccgggtcg tagggctccg acatgtcggg aaaccgcggg cccatcgcct cgtcattggg
     4561 tccgatcagc ggattgtcgc ccatgaaatt gacatggtcg tccaccagca cgatcgaccc
     4621 ccgccggata agcggattca tgctcccgac ggcattcatg acgatcagcg cctcgcagcc
     4681 cagcgcccgc atcacccgga ccgggaacgt cacctcgcgc agcgagtagc cctcataacg
     4741 gtggaaccgc ccctgcatga cgacgaccgg ctgggccgag agccgcccca gcatcagatt
     4801 gccgctgtga gactcgacgg tggacaaggg gaagtgcggg atctgctggt acgggatgac
     4861 ggttttttcc tcgacacggt cggccaggcc ccccatgccg gtgccgcaga tgatcccgaa
     4921 ccggggcgct tcggcatgac gctcgcggat ggccgcggtc gcctccgcga tcgattgctt
     4981 gtattgttcc agatcaaagg cttccatcat gagtccctgg cggaaagtat ttcattcacg
     5041 catcgcgcgc cacggccgac agccgcgccc gcgccagatc cagattcatg ccacgcagga
     5101 gcaggtcctt ctggcgcgac cgttcgcccc gcagcaggat aacgtcggcc ggtcgcagac
     5161 cgagggcatc ggccaccacc cggcacagcg cctggttggc tttaccctct acgggcgggg
     5221 cctgcacccg cgccgtcagg cgcccttccc ggagcccctc cagtgccgag cgcgaagcgt
     5281 tggggagcag acgcacctgc aacaggcagc cctcggggtg ttcgcggagg cagggccact
     5341 ccatagaacc ggacatcata ggcacccgga cggcatgcgg gcaaggcaaa aagcgacaaa
     5401 acagcggggg ccgccggacc aacgaagccc cgcgattctg ttccagcgcc ggccatcttc
     5461 gcatccttta tgtcgtttat gtttcattat atccttttta tcgctgaatt cccccaacac
     5521 ttcgcttttg cctgtccttc ggatactata gaatggtcct gccgcgttgc acgggagcgt
     5581 gacggcagtc tccgggtcaa tcgaccatgg agggtctgga accgctcgct ggacaggatc
     5641 ggccgcggtc ggcgcgccag tttcccgccg cctgcccacc cccgatccgc cgccgctccg
     5701 gggatcgcct ggacaccatg acgggagtcg atgatgacga agatactggg cgtggtcggc
     5761 agcctccgca gccgctcgca tacgcgcaag ctggtcgcgc tggcgctgga ggaggccgcg
     5821 gcgatcggcg ccgaaacccg cctgctcaac ctggaggaaa cgcccctgcc gatgttcaac
     5881 cccgagcgcc gcggcgccga cccggtcgaa cagcaggtgc gcgagctcgt cgtctgggcc
     5941 gacgccttca tcctcggcac ccccgactac cacggctcga tgtcgggggc gatgaagaac
     6001 ttcctcgatt tcttctggaa ggagttcacc ggcaagctgt tcgcctacat cgtcgcctcc
     6061 cacgagaaag ggctcacggt gcaggatcag cttcgcacgg cggtgcgcca gtgttacggc
     6121 tggagcctgc cctatggcat cggcttcaat ggcgatgcgg tgttcgatgg caacgatgag
     6181 ctgatcgacg aagtcgtcgc cgaacgggtc cggatgctgg cccacgatct gacggtctac
     6241 ggcgggctga tccacggtca gtttctgcgg gagatccaga acgaaccgcg ggcgcctggt
     6301 ttcgcgcgca acttccggcc ttgatctatt ccagtcagcg gccgccggcg caggcggctg
     6361 atttcaagcc agcccgccgc gcgcgcggcc cggcgaaatc tccattcagt ccgcgtggcg
     6421 gtagcgccgc gggttgatct gaccggcgag gcagagcagt tcataggaga cggtcccggc
     6481 ggcgcgggcc gcttcggccg ccgtcagcac cgcgctcccc tgccgcccga acagcaccgc
     6541 ttcatccccc acctgaacgt cgcagcactc cgtcacgtcg accatgaact ggtccatgca
     6601 gatgcggccg aggatcgggc agcgccgccc gcggagcagc acgtggccgc ggttgccgag
     6661 cgtgcgcagc accccattgc catagcccgc gccgatcagc gccagttcgg tctcgcgcac
     6721 ggtgcggtgc gtctggccgt acgagatcgg ccagtccggc ggcacgtgct tgcggtagat
     6781 gacgtgcgag acaaatgtca tcacgggctc cagtcccgcg atcagactgg aatcgccgta
     6841 gatcgagatg ccggcgcgga aatcgtaatc accccccgcc tgaccgtcga tcagggcgac
     6901 ggcgtcggag ccgcccaggt gcgccggcag gtccagcagg ccggtcatgc ggcgcaaccc
     6961 ctcgagcagc tcaatctggc gccgggcgct ttccggatcg cccttgggat tggccaggtg
     7021 ggtccagacc gactccaagg agaggttggg cagcgcgcgg attcgcgcga tctggctcgg
     7081 gagttcctcg acgcgccggc ccagccgccc catgccggta tcgaccttca ggtgaacgcg
     7141 ggcgagcttg cccagccgac cggccgcttc atcggccgcg cgcgcgtcct ccaggtcggt
     7201 gatcgtcagg gcggcatcga ggcgaatggc ctcgcccagc gcctcgccgg ccagcgtcgc
     7261 gaacagcagc agatcgccgt cgtagcccgc ctcgcgcagc gcgatcggct cctcgatcgt
     7321 catcaggccg agcgtgatgt cgcggcctga ccactggcgc aactgatcaa cctcgccggc
     7381 gacggccagc agcccatgac cgtagccgtc cttcttggcg gaaacgatca gccggcggcc
     7441 gggcgccagc cgctcgtgaa cccgccgcag gttgcgccgc agcgccggca ggtcgatcaa
     7501 gcaccaggtc cgcgcccagc gcgccgtttc ctcgcgcagc cgcgcggtga acggctccat
     7561 cccctcggag gcgttggcgc ggcggcttgt gagcttgtct ccctgttcgg attctggttt
     7621 catgacgttt actgcatcgt gccgcccggc ggagtcggtc caaggaacgt cgatgttaac
     7681 gcagacgaac ggaaagataa acaagctcga cgcggcggat cggtcggttc acgactggta
     7741 tcggttcgtg ctgtcgtttc ctccgcacct ggttcgtggt tatctggaga agttcggcgt
     7801 cgacggctcc ggattggtgc tcgacccctt ttgcggcacg ggcacgacga tcgtcgagtg
     7861 caagaagcag gggatcgctt cgatcggaat cgaggcgaat ccgatggccc acttcgcctc
     7921 caccgtcaaa acaagctggt ccatcaaccc cgggaagctg atcacctggg ccaacgaaat
     7981 tgccgattcg gcgtccgggg aattcgagcg ccagggaatc gccgatgtgg agctgttcga
     8041 ttcaaacgac ggccgcgaac ggtatgagat gcggaccctg cccgcggaac agatgaagct
     8101 gctgttgaaa aactccatca gcccgcgccc gctgcacaag acgttgatcc tgcgcgacct
     8161 gatcattggc tcggtggatc gaagctgcgc cgcatacggc cggctcgcgc tggcaaagtc
     8221 gctggttgag gacatcggca atctgcattt tgggccggaa gtcggggtgg gggtgatcaa
     8281 ggaggacgct cccgttattc aatcctggct cgcgaataca attgggatgg cccgggatct
     8341 ggagacggtg cgaccgcgcg ggaaaacgga ggccacggtg cttcacggcg acgcgcgcga
     8401 tctctcgcat cttgtccgcg accgaaagtt ctcggtcgtc tttacctctc ccccttaccc
     8461 caatgaaaag gactataccc gcacgactcg cctggaatcg gtcctgcttg gcttcatcaa
     8521 cgacaagagc gaactgcggc gaatgaagca gcacctgatc cgatccaata cccggaatat
     8581 ctatcaacag gaccgggatg agcagtgggt taagggcaac aagcgagttc aggacctggc
     8641 cgacaaaatc gaagctcgac gaattgagct gggcaaaact tccgggtttg agcggctcta
     8701 cgccagggtg acccggtcct actttggcgg aatgtaccgg catcttgccg atctgcgcga
     8761 ctttctcaag ccgggcgcct ggctgggcta tgtcgtcgga gatcaggcct cctatctccg
     8821 catcatgatc cgaaccggcc agattctggg cgaaatcgcc gaggaactgg gctaccgcgt
     8881 cgagagtatc gatctgttca gaaccaggct ggccacggcc acgcgcgaac aactcaatga
     8941 ggaggtgctc gttctgcgat ggcccggatg aagcctgaaa ctcccgtgtc cagatactcc
     9001 gagctgatcg aagcggtttt tctggagcat tattcgcccg gcgtcgacca gttcaccttc
     9061 gatcgcgatg agcttgtgca acatgccaga cgacggcgaa tccggcttcc caaaaacctt
     9121 ggcgacgtca tctacagctt tcgatataga aagaacctcc cggaaaaaat acaggcaacg
     9181 gcgccggagg gaatgagttg gctgatccga tcggcgggtg tcggtcgcta tcggttcgtc
     9241 ctgaccaagg gcgaaatccg tttcaagccc aacgcgttga tgtcggtgac caagattccc
     9301 gactcgactc cgggactgat ctcgcgctac gcgctgaacg atgaacaggc gctcttggcc
     9361 atcattcgct acaaccgact gcttgacatc ttcacccgcg tggcttgcta ttcgctacaa
     9421 agccatttgc gctcgtcgtt gccggctatt ggacaggtcg aaaccgatga gatttatgtc
     9481 ggcgtcgatc gccagggggc gcattacgtc ttccccgttc aagcgaaagg acagaaggac
     9541 acgctgggca cgctccagat cgagcatgac atacacctgt gtcgccataa gtttccgggt
     9601 ttgatctgcc ggccgatcgc cgctcagttt gcgcggggcc ggaccgttgt gctcttcgaa
     9661 ttcgacgaca atgcacagga actgcgcctg gtgcgggaat gccattatcg gctggtcaac
     9721 cccgacgaga tttcgcggga agaaatggaa cgctatcgca ctctgagcga tgagtaacga
     9781 tgtacgcgca ggccgtcacg atccccgccg cgccgtcgga gcccagcggt ctgcccgagc
     9841 cgcagacgcg cggctacttc ttcaatctgg tgcgcagcat ctcgccgacg tcgctcgagt
     9901 tggagctgcg gcgggtcgcc gaggcggggt tcaacctcgt cgtcttcccc gtctacagca
     9961 acggctggac gctgttccag tccgccaccg cgcgcgaata tggcctcccc tcaatcaatc
    10021 cgcgcctcag caaatgggac gccgtcgagc aggtcatccg gctcgcggat cgtttcggcc
    10081 tgaccgtctg gggcttcgcc cgaccctata atttccaccc ccgctactcg atcgccgaac
    10141 accggctgct ccgcaaacac cccgactggc gcctgcgggc gcatccgcag catctgaccg
    10201 cgcgcacccg ccgcgccgaa tcgctccatc cctgcccgct caaccccgac taccgccgct
    10261 tcgtcggcag cctgctggcc gaactagccg ccggctacaa tatccccggc atcgtcatca
    10321 actatacggc ggtcggcatc gagggcggcc cgatcaaatc ctcccccttc tgcttttgtg
    10381 cctcatgccg cgaacggttc cgcgaaaccc acggcgtcga gctggcggcc gaggcgctgc
    10441 acggcgacct ggaactggtg cgcgcctggc agcgcgacca gatgcacctc catttgcgct
    10501 acctgcgcca tcggctgctc aagatgcgcc attcgctgcg gctggtggcg cgcgtgcggc
    10561 cccaatggcg cgagggggcg ttcgattcgc aactgctgac gcccggcgtc agcctgctcg
    10621 actggtcgac cctggcccgc gaaggggcgc tcgaaacgct ggtcatcgat cacgacaacg
    10681 agcggctcgg cccgctgttc agcacgcggc tggccgccga ttacgcctac ctgggcgatc
    10741 aggtcctctt tctgccgatg ctttcggttc acgacctgga cgatctgagc atcccgatgg
    10801 cggtcctccg ccgctatccc tgcaccggtt tcatcggcga gttccagcgc agcttcaccg
    10861 atggcgacac gcagttcctg gcgcgccagg tcttcggcaa tccggcgatc ctcccctccg
    10921 accggccact gcaaaccgcg gcctacttcc tcaatctggt tcatcagacg catcaggatc
    10981 agcccgaact gcgcgaactg acccgcgata tcctccgcct catctcgcgc cagtcgcccc
    11041 acccgcacga ctttgaactc ctgcgcgtga tggagcagaa tctgcgcggc ctggaacagg
    11101 cgatccgtcg cggcgcgctg ggctttggtt ccatccccga gcggacgctg attcacctcg
    11161 gcctggcccg ccgcttcgtc cggatcgcgt tgatggatgt ccccacctga tgcgtcccgc
    11221 gttcaaccgg cacctcgggc agcgaccgct cgtcgcgctc cttctgtgct atctcctgct
    11281 ggcgctggcc atcctccatg gcggcggacc ccggcaggcc ggttcgctgc gcggcaccgg
    11341 catccgcgaa gcccccgacg atgaggccgt tctgcccggc atcctgcgcg cgccgctgat
    11401 gctgacgcgc accgtcccgg ccggctccga cacgctcggc catgactatc ccgcctggct
    11461 gtggggctgg agccagatcc gccggacgaa acagatcccc ctgtggaatc cctgcctgtt
    11521 tgccggcatg ccgttcctgg ccagccagat gttcatgccg ttttatccgt tcaactggct
    11581 gggggccgtg ctgccgttcc cattggcatt caacatccaa tatccgcttc acctggtgct
    11641 ggcggcgctg gtgatggcct tcaccgtccg ccggcgcagg atgacgtggt ggaccgcatt
    11701 cctggtcggc atcgcctggg gtttcggcag ccacctggcc acgctcgccg ggccgggcca
    11761 tctgcagaag ctgcaggcgc tcgcctggct gccgctggtg gcgtggggcg gcgcctgcct
    11821 ggggcggggc gcggggcgcg ccggcgctct cgccctcggc gcgggtctgg ccctgcagat
    11881 cacggcgggc cacttgcaga tcgtctatct ttcgctgctc gtcgcattgc tcgaggcgct
    11941 ggcggcgctg gcggtcgcat tcctccgccc acgcgatccg gcccccgcgg ccctcagccg
    12001 tctgcaacgg cgccgcgccc tcgcccggtc gctcggctgg acggccctcg ggctcgccgg
    12061 tgcgctcggc ctgtcggcgg tggtcctgat cccaaccctg gagttcgccc ggatctccaa
    12121 tcgccagggg tcgctttcgt gggacgaagc cactcgcggc tcgctccctc cggaggaaaa
    12181 gttcgaattc atcttccccc gcctgctggg cgacagcatg cccgcgggac gcggaccgta
    12241 cctgggccgc tacggcgaaa ccgatagcgg cgcgacgccg gagcggatcg tcagcgatta
    12301 cgtcggcgcg ggtgttctgc tgttcagcct gctcgcgctg gcgctgccgg gccgccggcg
    12361 cgtcgcgctg gcctggctgc tgctcgccgc cggcgcgctg atcctctcca tcggccgtta
    12421 ttacgcgggg ctctaccgct ttctgctgga ttggctgccc ggcctggcgc acttccgctc
    12481 gccctccacg tcgatggcgg tgctcagcta cgcgctggcc atggccgcgg cgcagggggt
    12541 cgagtgctgg ctggccgagg gccgaaaccg tgcccacgcg tcgcggggac ccgccggctg
    12601 gatcggccac gggatgatcc tgctcgcggc gggcgcggct gcgctggcgc tggtcgcggc
    12661 gttctggctg cgcgatgccc gccaggcgga tctgccgcgc gccgtcgcgc aagccgccgc
    12721 cgcgctccat accttccgct tcctggccct cgtcgccggc gtggcggggc tgtggcggat
    12781 ggtccgccgg ggcgagcgcg ccgcccggcc gtggattgcg ccggcgctgc tcggcgtgct
    12841 ggcgctggtc tgggcctggg accttgtgct caacgtccgc ccgttctgga ccgtcgaggc
    12901 ggcggcgccc tacgagcgcc acatccagca ccactgggcc gaacgcgtct ggcgcagcga
    12961 ggcgcggccg gtccggttcc tcgtcccggg ccgcgaactg cagaactggg ccatgaccta
    13021 caccggaacc ggcccagggc cgatcatcag ttcgatcagc ggctatcatc cggtcgtgct
    13081 cgacgtctat gaatcgttca tcgagcggct cggcttcctg catcccaacc tgctgcggct
    13141 gctggccgtc aattatctgg tctggcccga cgaccccgat ctcccgttcc ccgagggcta
    13201 cctgccggtc agccgcatga gcggagaggt cctgctgcac aacccgcaat acccctacgt
    13261 gcggccgatc cgccacttcc gcgaggtcgc cagtctggac gcattgctcg accggctgga
    13321 ggatccccgg tttcaacctt acgaatcgac gctcgtgctc gaagagggca agcacccgaa
    13381 gtccctgctc aaggggacgc cggaggcgac gcacctgtcg gccgaagcga cctcgatcgc
    13441 gccgggcgac gtgacgatcc agatccgcac gcgcaactgg ggcgccgtcg ccctcagcga
    13501 gctcgcggtt cccggctggc gcatccagcc ggtcgatccc gcggccggcg tgcgaatcga
    13561 accgttcatc gccgacgggc tcctgctcgg cttcgccttc ccggcgggcc gtcacacggt
    13621 gcgctgcacc tacgatccgg tcagccagcg gctggggctc tacatcacgc tggtgacgct
    13681 ggccgcgctg gcctgcgccg cggggcgaaa gctggcggag cgaagacggg ctggaactca
    13741 gccggcgacg ggcgaggcgt ggacgtcgta accgtcggcg cggttaatct ctacatcgat
    13801 gaactggccc ggagtgagcg gcgttgcgct ccagacgaac acccgggcat cgatctcggg
    13861 ggcctcggcg gcgccgcgcg cgatccagca gccggcgtgc ggctcgtggc tttccacaag
    13921 cacgcgctcg cgccggccga cccgcgcgcg gctgtgttcc gccgcgatct cggcctgcgc
    13981 cgccatcacg gcgtgccagc gccgttcttt ttcaccccgg cgcacgcgcg gccgcatcgt
    14041 cgccgacggg gttccttcct catccgagta ttgaaaggcc ccgagccgct cgaaccgcaa
    14101 ccgtcggatc ccctccagca tgaactcatg ctccgcccgc gtctcgccgg ggaagccgac
    14161 gatcatcgtc gtccgcagcg tgagtccggg taccgccgcg cgcaggcgct ccaccaattc
    14221 gaatgttttc acctcgtgga acgggcggcg catccgccgc agcgtctcgg gatgcaggtg
    14281 ctgcagcggc atgtcgaggt acggaacgat cttcggttcg gcggccatga cctccaggaa
    14341 gtcgtccgtc acgttgccgg gatagaagta gaggcagcga atccagaact cgccgggcag
    14401 ggcgcacaga tcgcgcagca gatcgggcaa ccgcacgtcg cgccagcggt ccatgccgta
    14461 catcgacagg tcctgggcca ccaggttgat ttcgcgcacg ccgtcggcga gcagccgctc
    14521 ggcttcggcc agcagaatct cgcgcggcac cgaccgcagc ttgcccttca tcgccggaat
    14581 cgaacagaac gtgcaggcgt ggttgcagcc gtcggagatc ttcaggaagg cgtgcggcgt
    14641 gtgctgcagc cgacgccggt gcatgagctg gtagatatcg actgtggggg tctcggccac
    14701 ctcccgcgtg cgggcctccg cccggtcggc gttcaggatc agattggcta gctggcggaa
    14761 ctggccgacg ccgaccaggc cgtcgatttc ggggatctcc tccatcagct ccgcgccgtg
    14821 gcgttgcgag aggcacccgg cgacgaacag tcggcgcggg ttgccggtcg cgcgcttgcg
    14881 ttcggccagc tcgacgatcg tatcgatcga ctgctggcgg gcgcggtcga tgaacccgca
    14941 ggtcgtcacg acgaccgcgt cgagcgggcc gtcggccgct tccccatcga cgaccagccg
    15001 gcagccgccg gcctcgagca gcccggccag gtattcgtta tcgaccgtgt tcttgtcgca
    15061 tcccagcgtg acgatgccaa catggagcgg tcgcgtggaa acgggggaaa ggggcatcgg
    15121 gaagcctgtc cgcttgcaat cgggagggtc gggccgcata gtgaggatgg ccgccgcggc
    15181 ggttcggcgc cggccgcacg cctccccagt ctatcagccc catgccccag ccgccaatcg
    15241 aaacacccga acggattcgc actcaaacgc ctccggcctc ggctgcaatg catcggcacc
    15301 tttggctggg gaagctcgtg atgcctcatc gctccgcccg gctcgacgcg ctcatgctcg
    15361 ccctcgtgct gctgttcgca gtctggctgc tcgggcggat gattgctgcc cttcatctcg
    15421 gagccgtcgc cgcagccttt cacaatctgc tctctctctt cacgcggccg tggcatccac
    15481 ccgtcgatgc gatcgcgggc tttgctgtcg ggatgatcct cttcgccgta ctcgtcggtt
    15541 tgggttacgc gattctcaca tcgacccgct ccgggcggcg gatgctgccg ctgctcagcg
    15601 agcgcgtgct gctggccccg ctcgtcggcg ccgtcccggt ttcactcatc attctcctgt
    15661 ggggcctggc cggctggttg ggccgggcgc cgttgattgc gctgctggcg ggattcggtc
    15721 tgctcagtct gcgcggatgg ctgcggctgg ggccggcgct ctgggcccgg cgtgccctgc
    15781 tggtccgttt catccggggc cagtcgcccc tcgcgcttgc gctgggcctc ctcgtcgcgc
    15841 tctggatcgt gccctacacg ctctcgccgg cggtcgagtc cgacgaactg cgctaccacc
    15901 tgagcgcccc ggcccaatgg ctggcgcgcg ggcgcatcga atacctgccc tggcaggcgt
    15961 tctccaactt cccgttcctg gccgaaatgc tctacctgct ggcgatgggt gtcagcggac
    16021 cgccctacca gtggctcgcc gaaccggcca ggctgctgca cctgaccatg ctgccggtga
    16081 acatgggctt gatcgcgctg ctggcgctgc ggctccggcc gcgcgcggcg cgccgccgtg
    16141 cccccgacac cagctccgcc tggtggttcc tgttcctgcc gtcggcgccg attctggccg
    16201 cctgggcctt cgtcgatctg ttcatgtcgg cttactttct tggtttcgtc tatctcggcc
    16261 tggtcagcct gcggcggccg cgccgcgcgg tgggaatgct cctgggcctg ctggccgccg
    16321 gcgccgtcgg aacaaagtat tccatgatcc cgctggtgag cgtgctgggg ctcttctggg
    16381 gcgggttgtt gatctggcgg agcggcaggc ggctcgccgc gcgccggctg ggcggcgccg
    16441 cgctcaccgg cttcctcttc ggcgcgccgt ggctcatccg caatctggtc tggaccggaa
    16501 acccgttcta tccgctggcc tggagctggt tcggcggcgg cgagtggtcg cgggagaaca
    16561 tggcgttcta tctggaaaag gccggggcga aggggatgca cctctggccc gaccgctggt
    16621 gggggccgct ggccgactgg ctgctcacgc cgtggaacgt gacgtttcac atcgaccgct
    16681 acgagcagca ttatctgggg ccattgccgc tgatggcgct ggccttggtc ctcggctggg
    16741 ggctggcggc ggcgatcccg aagtcgcccc gcccgcgccc gcccgggcgc cgaccggctc
    16801 ggctctggct cctcgccggc atcgtgattt catgggccgc ctggttcgcc acctatcaga
    16861 gcgcgcgcat gctcctgccg acgatcgggc tgctgtttgc cgtcgcgggc gcggcgtgga
    16921 acggctggat cggcgcgcag cctcgccgct cgctgcgcat ggccgcgcgc gcgctctggg
    16981 gcgtcgccgc cgtctactgc gtcatgtttc tcgttgcggt gctgggccag accgggcgcg
    17041 ggagcgccgg caagctcgac gccatcgccg tggggctcgg tttcgagcgg ccggaagtct
    17101 atcttgcccg gcggctcaat tacgcctccg ccgcgcggcg gctcggccag ctggccgcgc
    17161 ccggcgacaa ggcgctcctc atcggcgagc atcgcacgct gcacttcccc ctgccggtcg
    17221 tcgccagcga ctggttcgat accccccagc cgctcccctg gctgcggcgg ctccccgccg
    17281 acgacgacgt cttcgatgcc ctctggcaag tgggggtgcg tttcgtcctt ctcaaccgcg
    17341 cggaactggc gctctactat gaaaccgatt tcctcccacg cctgaagccg ggcgaggccg
    17401 agcgcctggg cgatcccttc atcccccacg gccgcggcgc gggattcctg cacagcccgc
    17461 gcctgacgcc gctcggcgaa cgcggcggcg tggcgctcta ccggctcagt ccgcccgctt
    17521 catgatcgaa ccgacatcgg caggattcga tttcgatccc gatagcgata ccgctaccga
    17581 ttttgacgtt catcgcaggc aatacagata tccatcctgc gtcggcacga ggatcgatgc
    17641 cgaccccgcc tcgcccagct cggcgacgat cggcgacccg actggcgatc ccagatcgac
    17701 cgtccacagc acctggcact gcccgtccgc ctccttcagc gcatacagct tgccgtccgc
    17761 cgcgcccgcc agcagctctt cctgtccatc cgaatccaga ttcaccaccg ccaggtcgct
    17821 gacccgaccc ggcaggctga accgccagcg ttcgctggcc gcctcccccg ccgcgacatg
    17881 gttgtaggcg atcagcagcc catccacctg gctcgtcacc agttccttca cgccgttgcc
    17941 gtccagatcg accacgcccg ggtaatgcgc cgccgtcgta tcgcgcgtca gcttgaccga
    18001 ccacagcggc tcggattccc acgtcgccac gtaggtcgcc gcgaacgcat gcgaatgcac
    18061 cagttccagc ggcgcctgcc catccatctc gaccagcagc agcttgctgt aggccggcca
    18121 gatcttgtcc gcgcgctgat cgaggatgtt tttcgccgcc agcagatcct cgccggtggt
    18181 ggcatcgatg atgccgaaga agttctccga cgccacgaac aggtcatccc cgccgtcgcc
    18241 gttgtaatcc gccaccgacg tcggcacatg aatcccgaat gtcgacggtt tcccgccgta
    18301 caatccgtag gtcgtccgtt cccacagcag ccggccgtcc gtcgcgtcgt acgtcgccac
    18361 cgtccacggc acgccccacg tcttctcccc ttcctgccag cggatcgtga tccagttgcc
    18421 cggcttgccc gccagcttgc cggtcgccac caacaccatc tctcccggat catcgcccgc
    18481 cgtcagccgc agtttttcct gacccgcggc atcgaccgcg acgaccgcgc ggccttgctc
    18541 cgccgtggtc gtcaacagct cattctcgcc gtcgccgtcg acgtcgccga tcgtcccgat
    18601 gaacgtcgta tgcaggtgca cccccccgct ggcgggtgtc tgccggacca gcgtccgctc
    18661 atccccgccc gcggcgtcga gc
//
LOCUS       DAOPCO010000046        18416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3123_length_18416_cov_5.719678, whole genome shotgun sequence.
ACCESSION   DAOPCO010000046 DAOPCO010000000
VERSION     DAOPCO010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 18416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18416
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_18416_cov_5.719678"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..679)
                     /locus_tag="PLS90_05430"
     CDS             complement(194..679)
                     /locus_tag="PLS90_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPK01878.1"
                     /translation="MSAADKPSYQPGDWVEILGMHYGRGRVLAVDPRGRRVRVRIGDQ
                     DWTLKPDQLKPTAPPDPAERPRPVHGLHSTGAVQHQVDLHGMTVEEALLAAEKALDQA
                     LVSGLFQFKIIHGHGSGRLRQAVRAMLAGHPFVKDFRFGEPHEGGLACTVAFLRHERE
                     P"
     gene            complement(778..1665)
                     /locus_tag="PLS90_05435"
     CDS             complement(778..1665)
                     /locus_tag="PLS90_05435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPK01879.1"
                     /translation="MAILEIPLNQRVRIPAIRLSNGQPFDGKVVASDGELLVLELAAD
                     CRGVMTGPAETAAVMTWSSDGIQRACPIMVESNNDRRLTCRAVIQEKRESPRLRVEVQ
                     VAYERVAAELVRETADEVMAKISTPAAAEAELGELLRGGQDDPLRKLRDEVGGLHDML
                     SEVLLKLDNLMTLIASGERAFVPGLRSPLCVLNCSSTGIGFLTDEELHEGEYLRIRMT
                     LRTMPQTQIECMGTVIRCRPLNQGAIGEVPRYDVGVHYTHIHESDREKLIHYLFRAQR
                     QMLRDRKEAREALRSGAEK"
     gene            complement(1861..6699)
                     /locus_tag="PLS90_05440"
     CDS             complement(1861..6699)
                     /locus_tag="PLS90_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPK01880.1"
                     /translation="MSFLTIGVDLAAQEARPLKLLWEGDSEFAHAPSDPLAFDVDLDG
                     RMDLIQVGLRDVVEVLDPTHGAPIWRQQLGEDETLLSPVAGNFLGNDRITIVVPSTSG
                     KLFILDGPTGRQEMPAQQTGFGLTLPPATFPLTGEDEIAPYREGILLYNPSERTLVGC
                     QLRGPGHAQSLFELFRHPTQGILERGPSVGQTRFAGGRGPHFVYVTREGKVTVFNRAM
                     PPAAITLNLVSTNRTYGLGQALGDLDGDGYDDIVIADSLGYLYGLTVRDDAIVPVWAP
                     PAGGTEADAHRSIGSEPRFTPVTVDANADGKDDVLVVRDRALYMVDGASGEPFWESAA
                     LVGRLHERAIESPPAIFFDDSRTAYAVFCDVICMVVINLRSTETWGQYDLGSQVVNTP
                     LVGPLTGGGAAQAFARADMSGRVHCVDLGLNFLQGSPAWMGRLGGPSRSGSANRAFYH
                     DFRNRQQQRLSTFLTQILNHARTLAEAGQYDAALAEVRKVREISPPHREARQLERQYL
                     IRANLIWMLTAGVLLLGLVGFVGFHLFRHTRAAIQQMLARMAIERRQYPRAIRLLRRL
                     TATFPRRRRYVRDLADLYVQLKQFDAESALIFERAREMFPREDRYLKGLATAYSSIPR
                     NDEAAARVYAEMSRVMKKPGPWFFLLGQTLMQIDRPREALEAFRQTIVHQHDDPKLPH
                     YMTDLYIKLEIAAPEIIPTLDRVFEQRVEDRAFLRLYCMACQAARRYDERAQQVAEML
                     LELDPAAPPAHVILSTRLLQGGHNKDAMLHAQQILQVNPSDSMGLRLLGACYAAENRL
                     DQTAMKIFIRALQANPDAPEILTAVSHGYAQEGRQDRDAREIYKKSLMHNPQDETVLV
                     QLAQIAETEGDDDLTIRAIEPLLGLGRRTREMVLQLANAYCRQGIVEAKAEPIYREAL
                     VYQPDHATIQDNLAAIYLHKKKVDNEAAQVYAAVFERHPERFDIGLQLMRCHVAAEHP
                     ERALELGRRLQERDPDNGELQKLMAASSDRANQMDSAIQAYEQVLANSPEDPEPVCAL
                     SSLYFRKRRHDNEAIAIYNRAIQLQPQNHEHYMAAAQAYAARETWDHAIAIIKQLLTH
                     VPTQIGAAIGLMEALIESSPKVLKLRWYLVDTLIFDGRLREARQHLTEIQRIDPGDPE
                     RALQAFDKILEKNPRDAMAHLERGRLLLLAGKEREARKALEDAHRFHPENEEVIRNLM
                     KLYQKILESRDSAEVRFQLGRLAMRMDKYDLAISCFQQTSRDYRWEGESVRNLARCFM
                     AKGMLDLALQELKRLPVEKDVKELLYELGQRYESVNDVAGAREVYKLIFAADITYRDV
                     KGKLETLNEANADQMGAERTAIINSLSEKAKQRYELVEELGRGAMGIVYRAHDNELEE
                     EVALKILPDSLLRNQEAVRRFRQEARNARRLAHPNIVRIHDIGEELGRKYISMEFVKG
                     TDLKQKLRRVNRKLPLESTLRYMRQICEAMAYAHSIGIVHRDIKPANLMLDENENVKV
                     TDFGIAKMVEQSEVGSTMAGAIIGTPLYMSPEQVKGQTVDHRADIYSMGIVFYELVNG
                     HPPFMEGDLAYQHLFVEPKPLTNVSPELAEIIMKCLAKEKEKRWQSAEEMLRALDNLN
                     GV"
     gene            7048..8220
                     /locus_tag="PLS90_05445"
     CDS             7048..8220
                     /locus_tag="PLS90_05445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01881.1"
                     /translation="MTAPQATLPPQPAPRPPDARARIATRRVTLMHDLLLGMRGGEWV
                     LDSLLKILPLAGIRTLFYRPRFIDTAINCRPIHPSPLSRLPGVRRYYRWLLPLLPPTV
                     ERLRVPERTELVLTLSHCVAHGIRAPRGVPQVNYYFSPMRYLYDQREAYRRHGGLGGR
                     ALGWLAPRLARWDFQAAARADHRWAISQFVARRIEDAYGLTARVIYPPVRTGFFVPPP
                     PDRAAERIPEYLLVSAMVPYKRVDLAIRAANRLRLPLRVVGDGPLLRQMRRLAGPLVA
                     VEGRVSQARLLELYQTRAALIFPSEEDFGLAPLEALACGMPVLGLRAGGLLETMREGR
                     TGAFFDRPTVKSLCEAWEQFRPAAYDPLHLRAHALAFSEERFHEEIALAIDTVLNE"
     gene            8306..8977
                     /locus_tag="PLS90_05450"
     CDS             8306..8977
                     /locus_tag="PLS90_05450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01882.1"
                     /translation="MQIIGNPRFLKSPRITWARWRLGHPAPGPIRVHPVGGFTFEDGA
                     ELTQRLTAAFGDLAGLSCLDLGAGPGETVIARQILDIPWARLASVEAFHPYLNKLRQR
                     QAAARRHEVLDCRIEHVFGRFRAGEFDLALLIDVLEHFSRRDAVDLLLRLERFVNRGV
                     VIFAPVGIVEQDELDANALQRHRSHWLAEDWSRLGYQVEVYEAFHGQLSPPATAAWAI
                     KSWRR"
     gene            9109..10563
                     /locus_tag="PLS90_05455"
     CDS             9109..10563
                     /locus_tag="PLS90_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK01883.1"
                     /translation="MRRFCWKPAAPFRLGLVLALAAAAATLAAQPSDSPSSPSLDQLS
                     EKQVEALPEGPADFQQIIERAKRRVFPALVFVKPIVEDYGSGEKKKQEVFGSGVIISP
                     DGLVITNNHVVENAIKVNCVLFDKDQLPAEILGRDPDTDLALLQLDAPAEKRPLPAAE
                     FADSDAVDEGDFVMALGSPFGFTRSISLGIISNTQRYLGFENEYRYNLWLQTDAAINP
                     GNSGGPLVDIEGRIVGINTLGLRLVAENVGFSIPANVVREIAARLERDGAVSRAWTGL
                     RLQPLKDFNTNTFTQAERGVLIANVESGSPAEAAGLQSGDLLVAVGDHPIDGAYVENL
                     PAIHWRLADLPTDAPTTFTLVRNGRTIAAAVQPVLKGKVEGENLDLRRWNMTLKEINK
                     YKEPQLYFYRKEGIYVQGVRNPGNASAAGIQRNDILLSIERQPVDSLATARQLYESIL
                     GDETREKKVLLEVLRGGLPKLIVLDYTKDYEKAD"
     gene            10567..12924
                     /locus_tag="PLS90_05460"
     CDS             10567..12924
                     /locus_tag="PLS90_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPK01884.1"
                     /translation="MNGSFLARASRAVRLLALLGLACLARPCLAQPAAAASADAAPTT
                     GPLTLQQAESFLEHFAVIEVFFKRSEEDKDAVHPEYESYISRKLPMRLIGIALDAEHV
                     VTIDTDLDEKWIDRIQVTDMRGRSAPAERAALLLDAPALVFRVADPTGLTLRPPQFTD
                     PGPAAPDGLQQLVVTRHGSNYMLQLSPAQPLVQLTPEYQLQRVLSTAVYDPDASSRGM
                     MDDWSGWSSDFEINKVPVLLLDQRRRPVGLAFRAYLPYGESAEEGLRPDQRERVAWAQ
                     YAAAQDQLTTAAGRWVMETHITFRQKAEEGGGTFLGLPLPNVDSIMGGQVNVPDEWYV
                     YGFPIAPDLLFVPTRIQREFAKVIDRIEVRDGDRTIPGRFYGAYEDIAAFLIRLDEPL
                     SVAPAEIFSSQPVRMIRPMLSIYPERRYGREYLDVGYARLLSRLNGYRDVLEFVPAYE
                     ARVGALYTDLDGNLQGVTLEQRKEDEDKERFRRAGGFPYFGGGETFYRFYSIPELAPY
                     FNAPNDHLDPTIVSLSEEEEERKMWLGVEFDPMTQELAKTLDVQPQTKDGTIGLWVTR
                     VYPGSPAARLGLERGSILLELREEGEAQPVELKAADTSMYDFDFSDVELPEGMENLGI
                     SLPRAKPWKNRRNMLTRLLEVIGEGRTVHLRYLDPQRAEHSVTFAVEKAPHDFASAPK
                     YKDEEIGLTVKELTYEVREALMLPPDARHLVVSSVEEGSPAAVARVELYDLIIAINDQ
                     PVPDLETFKRIAQDARAEREASGRATLRVTLQQLDKTRIVDLTLE"
     gene            complement(12937..13407)
                     /locus_tag="PLS90_05465"
     CDS             complement(12937..13407)
                     /locus_tag="PLS90_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPK01885.1"
                     /translation="MPLPDHYATLGLKPTATFHEIKQTYRLLVRRHHPDRHGGDARAE
                     QRMAAINAAYEVLHRPERRMEYDRHFYEAFPHRIESAESTRQAYRDANPYIDALWRER
                     ERRMQRERGNLGGRITLYVLLLAVALYAVAFGLWSALQPPAEAGRSEATVQPVR"
     gene            complement(13540..14973)
                     /locus_tag="PLS90_05470"
     CDS             complement(13540..14973)
                     /locus_tag="PLS90_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPK01886.1"
                     /translation="MWEAIAANRRRSRLLLALMALLLAALGGAIGMSIDPDPATGGVF
                     GVLAALAVFGVMTLVSLTAGEAILLKSMGARKLETPAEARRLFNVVEEMTLAAGLPRR
                     PDVYVLDTDSLNAFAVGRNPERAAVAVTIGLLRKLNRDELQGVVAHEIGHVRNLDIRF
                     LTLAAVMMGSIALISDVFLRAFLHGGRHSSRRGRDSRDGGNQAQAIMLLVALALAILA
                     PLCAQLLYFACSRRREYLADASAARFTRFPEGLASALEKISGRAGGRLLGKQNRAVAP
                     MFIVNPLRAMGGGGLFATHPSTDRRIAILRAMAGGAGFAEYERAWRERAGERGALFGE
                     RTRAEEGERVPRRAAAPAEEQRDAVARAREALRLMDVMAGLLILPCACGLTIKLPPNY
                     SEPKIQCPRCGRTHFSNAARPALKAEAEVAVPAGAAAETEQIHRRGGSGWETIACRCG
                     GHVSLSPTFSAERVECPRCHRTIRVTD"
     gene            complement(14980..15546)
                     /locus_tag="PLS90_05475"
     CDS             complement(14980..15546)
                     /locus_tag="PLS90_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPK01887.1"
                     /translation="MGGALIALLVIGGLVLLVILWFIATYNGLVRLRNQVKEAWAQID
                     VQLKRRHDLIPNLVETVKGYAGHERQTLEEVMRARAAAVSAHGVGEQAQAESQLSGAL
                     GRLLMVVEQYPDLKANQNFLALQEELTSTENKIGFSRQFYNEQVMQYNTRIQQIPANL
                     VAGMFQFREEAFFELEDKSERAVPQVKF"
     gene            complement(15552..16004)
                     /locus_tag="PLS90_05480"
     CDS             complement(15552..16004)
                     /locus_tag="PLS90_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HPK01888.1"
                     /translation="MCPHCGAPMLVLELHGVEIDHCPQCGGTWLDGGELEQICDLAGA
                     PPGPLTAALNAGRRGRRGARRCPRCGWRCRGLTLAAEPPLELDHCPRGHGLWFDAGEI
                     RRLIEHAGGDEAGALADFLGDVLATEFRSERGPRNFLPSAAEPPRQEG"
     gene            16359..17924
                     /locus_tag="PLS90_05485"
     CDS             16359..17924
                     /locus_tag="PLS90_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPK01889.1"
                     /translation="MASSFLLSLSSARAATDRAARPAHPHRLRLIVTVPSVLILFTLG
                     YGLVSYQTFSSHWEVLESLGAGEVAATLLRVHLTAMLVLSLLAGLIGIALTDMILRPL
                     RSLMETARRVGEGQLDATAPSLPGASEFAELSQSFNRMLQDVNESIARRNRCLVDTIP
                     IGVLTTNLEGVVTTINPAGARLLALAPEQVRGRRLADLAQAVPPDARELIERFQTILE
                     GPEPTFQGSLELPADGPAGVRRMTVASSSLRDADGSPWGLLFSFRDAGAVETLSAHLS
                     HTDHLAALGTLALGLAHELRNPLAAVKGLSQLLQSERDLPDSTRKYLERMTREVDRVD
                     LFVKRLLELGERSAQGPTACDLGRLLTEADRRARAEAREAAAHVARDLELQPLPPLMV
                     ERDRLEQALFQILLNAYQCTPPGGRIRATTSAGFDDGGRLRFRIAVHNTGSTIAPADR
                     RRVFEPFFTTRDQATGLGLTYAHQIVSQNGGELTLEVEEDGVRFILQFGEERRAISSE
                     TETASNPREGEQP"
     gene            17921..>18416
                     /locus_tag="PLS90_05490"
     CDS             17921..>18416
                     /locus_tag="PLS90_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK01890.1"
                     /translation="MSATFHDPSRPAMGAPGTPGPNPLAGDPLELGRADGYRVLIVDD
                     EDTIRFILRELLEREGCIVDEAENGVEALNMARRNGYDLYLLDMKMPEMDGLAALRQI
                     RRIYPDALAVMITAFGSNQLALDCLKAGAYDYFTKPFNIEELRILLLRALEKHHRDLE
                     DFFLD"
BASE COUNT         2873 a         6535 c         5887 g         3121 t
ORIGIN      
        1 cgggcggcag aggccgccgc gggcgacccg ccatcatcac gggcgtgttg ttcagggcat
       61 tcggcatccg gcgttcttcc cgtcccgtcg gttctccggg atcttcgcgg ccatcccgcg
      121 aatgggccca tatcctaatg aatgggccgg cgaatttcaa ggctgatttt cattattttt
      181 tcgccggcgc ccctcagggc tcccgttcat gccgcaggaa ggcgaccgtg caggccaggc
      241 ccccctcgtg gggctcgccg aaccggaaat ccttcacgaa ggggtggccg gcgagcatcg
      301 cgcggacggc ctggcgcagc cggcccgagc cgtggccgtg gatgatcttg aactgaaaca
      361 aaccggaaac cagcgcctga tcgagcgcct tttcggccgc cagcagcgcc tcctcgaccg
      421 tcatcccgtg caggtcgacc tgatgctgga cggcgcccgt gctgtgcagg ccatggacgg
      481 gtcgtggccg ttcagccgga tcgggcggcg ccgtcggctt gagctggtcc ggtttcagcg
      541 tccaatcctg gtcgccgatc cggacgcgga cgcgccggcc gcgcggatcg accgccagca
      601 ctcgcccgcg gccatagtgc atgccgagga tttcgaccca gtcgcccggt tgataggaag
      661 gtttgtcggc agcggacatg agcggctcca cgggcggccg gccggacgcc cgcgccactc
      721 actctaggag gcgatcgccg attgagccaa taaaatgagc cggctgggaa aaggttgtta
      781 cttctcggcg ccggagcgca gcgcctcgcg cgcttccttg cggtcgcgca gcatctggcg
      841 ctgcgcgcgg aagaggtagt gaatcagctt ctcgcggtcc gactcgtgga tgtgcgtgta
      901 atggacgccg acgtcgtagc gcggcacctc gccgatcgcg ccctgattga gcggccggca
      961 gcggatgacg gttcccatgc attcgatctg tgtctggggc atcgtccgca gcgtcatgcg
     1021 gatgcgcaga tactccccct cgtgcagctc ttcgtcggtc aggaagccga tgccggtgct
     1081 ggagcagttg aggacgcaca gcgggctgcg caggccgggc acgaacgccc gctcgcccga
     1141 cgcgatcagc gtcatcagat tgtcgagttt gagcagcacc tcgctcagca tgtcgtgcag
     1201 gccgccgacc tcatcgcgca gtttgcgcag cggatcgtcc tggccgccgc gcagcagttc
     1261 gcccagttcg gcttcggccg cggccggcgt gctgatcttg gccatcacct cgtcggccgt
     1321 ctcgcgcacc agttccgcgg cgacccgctc gtaggctacc tgcacttcga cgcgcagccg
     1381 gggcgattcg cgtttctcct gaatcaccgc ccggcaggtc agccggcggt cgttgttcga
     1441 ctcgaccatg atcgggcagg cgcgctgaat cccgtcgctc gaccaggtca tcacggcggc
     1501 ggtctcggcc ggcccggtca tcaccccccg gcagtcggcc gccagttcca gcaccagcag
     1561 ttccccgtca cttgccacaa ccttgccgtc gaacggctgg ccgttgctca gacggatcgc
     1621 cgggatgcgc acgcgctggt tgagcgggat ttcgagtatc gccatcagtg gaccacccgg
     1681 tgaacaaatg gctcggcctg gccatgcaga cccggacgga cggccgtcgt ccctcagagc
     1741 ttatcggccg ccccccggcc gaactggagg gtgaccggtt ttggtaaaca gggcggttga
     1801 agtgagaaaa tgcgccggga cggtcggaac ggtcgggacc gacgattgtt caccgatccc
     1861 ctacacgccg ttcaggttgt ccagcgcccg cagcatctcc tcggccgact gccagcgctt
     1921 ttccttttcc ttggccaggc acttcatgat gatctcggcc agttccggcg agacattggt
     1981 cagcggcttg ggctcgacga acagatgctg gtaggccagg tccccctcca tgaacggcgg
     2041 atgcccgttg accagctcat aaaagacgat ccccatcgag tagatatcgg cgcggtggtc
     2101 gaccgtctgg cccttgacct gttcgggcga catgtagagc ggcgtgccga tgatcgcccc
     2161 cgccatcgtc gaaccgacct cgctctgctc gaccatcttg gcgatcccga aatcggtcac
     2221 cttcacgttc tcgttctcat ccagcatcag gttggccggc ttgatgtcgc ggtggacgat
     2281 cccgatcgag tgcgcgtagg ccatcgcctc gcaaatctgg cgcatgtagc gcagcgtcga
     2341 ttccagcggc aacttgcgat tgacccggcg cagcttctgc ttcaggtcgg ttcccttgac
     2401 gaactccatc gagatgtact tgcggcccag ttcctcgccg atgtcgtgga tccggacgat
     2461 gttggggtgg gccagccgcc gcgcgttgcg cgcttcctgg cggaaccggc ggactgcttc
     2521 ctggttgcgc agcaggctgt cgggcaggat cttcagcgcc acctcctcct ccagctcgtt
     2581 gtcgtgcgcg cggtagacga tgcccatcgc cccccggccg agttcctcca ccagctcgta
     2641 gcgctgcttg gccttctccg acagcgagtt gatgatcgcc gtccgctccg cgcccatctg
     2701 atcggcattg gcctcgttga gcgtctccag cttgcccttg acgtcgcggt aggtgatgtc
     2761 ggccgcgaaa atcagcttgt agacctcgcg cgccccggcc acgtcgttca ccgattcata
     2821 gcgctgcccc agctcgtaga gcagctcctt gacatccttc tccaccggca ggcgcttcag
     2881 ttcctgcagc gccaggtcga gcattccctt ggccatgaag cagcgcgcca ggttgcgcac
     2941 cgattcccct tcccagcgat agtcgcgact cgtctgctga aagcaggaga tcgccaggtc
     3001 gtacttgtcc atccgcatgg cgagccggcc cagttgaaac cggacctccg ccgaatcgcg
     3061 cgattccaga atcttctgat acagcttcat caggttccgg atgacttcct cgttctcggg
     3121 gtggaaccgg tgtgcgtcct ccagcgcctt gcgcgcctcg cgttccttgc cggccagcag
     3181 cagcagccgg ccccgttcca agtgggccat cgcgtcgcgc gggttcttct ccaggatctt
     3241 gtcgaaagcc tgcagcgcgc gttccgggtc ccccggatcg atccgctgga tttccgtcag
     3301 gtgctggcgc gcctcgcgca gccggccgtc gaagatcagc gtgtcgacca ggtaccagcg
     3361 cagtttgagc accttgggcg agctttcgat caacgcctcc atcagcccga tcgcggcgcc
     3421 gatctgcgtc ggcacgtgcg tcagcagttg cttgatgatc gcgatggcat ggtcccacgt
     3481 ctcgcgcgcg gcgtaggcct gcgcggccgc catgtagtgc tcgtgattct gcggctggag
     3541 ctggatggcg cggttgtaaa tcgcgatcgc ctcgttgtcg tggcgccgct tgcggaagta
     3601 gagcgagctc agcgcgcaga ccggctcggg gtcctccggg ctgttggcga gcacctgctc
     3661 gtaggcctgg atcgccgagt ccatctggtt ggcgcggtcc gaactggccg ccatcagctt
     3721 ctgcaactcg ccgttgtcgg ggtcgcgctc ctggagccgc cggcccagtt ccagcgcgcg
     3781 ttcggggtgc tcggccgcca cgtggcagcg catcagttgc agcccgatgt cgaaccgctc
     3841 cggatggcgc tcaaacaccg ccgcgtagac ctgcgctgcc tcgttgtcca ccttcttctt
     3901 gtgcaggtag atcgccgcca ggttgtcctg aatcgtcgca tgatcgggct ggtagaccag
     3961 cgcttcgcga tagatcggct cggccttggc ctcgacgatc ccctgccggc agtaggcgtt
     4021 ggccagttgc agcaccatct cgcgcgtgcg gcggcccagc cccagcagcg gctcaatcgc
     4081 gcggattgtc aggtcgtcat ccccctcggt ctcggcgatc tgcgccagtt gcaccagcac
     4141 cgtctcatcc tgcgggttgt gcatcagcga cttcttgtag atttcacgcg catcgcggtc
     4201 ctggcgcccc tcctgcgcat agccgtgact gacggcggtc aggatctccg gcgcgtcggg
     4261 attggcctgc agcgcccgga taaagatttt catcgccgtc tggtcgagcc ggttctccgc
     4321 cgcatagcac gcccccagca gccgcagccc catcgagtcg ctcggattga cctgcaggat
     4381 ctgttgcgcg tgcagcatcg cgtccttgtt gtggcctcct tgcagcagcc gcgtggagag
     4441 gatgacgtgc gcgggggggg ccgccggatc cagctccagc agcatctcgg ccacctgctg
     4501 ggcacgctcg tcgtagcgcc gcgccgcctg gcaggccatg cagtacagcc gcaggaacgc
     4561 ccggtcctcg acccgttgct cgaagacccg gtccagcgtc ggaatgatct ccggcgccgc
     4621 gatctccagc ttgatgtaca ggtccgtcat gtagtgcggc agcttggggt catcgtgctg
     4681 atgcacgatc gtctggcgga acgcctcgag tgcctcgcgc ggccggtcga tctgcatcag
     4741 cgtctggccc agcaggaaga accacggccc cggcttcttc atcacgcgcg acatctcggc
     4801 gtagacccgg gcggcggctt cgtcgttgcg cggaatcgag gagtaagccg tggcgagccc
     4861 cttcagatag cgatcctcgc gcggaaacat ctcgcgcgcc cgctcgaaaa tcagcgcgga
     4921 ttcggcgtcg aactgcttga gctggacata caggtcggcc aggtcgcgca cgtagcgccg
     4981 ccggcgcgga aacgtcgccg tcagccgccg cagcaggcgg atcgcccgcg ggtactgccg
     5041 gcgctcgatc gccatccgcg ccagcatctg ctggattgcg gcgcgcgtat ggcggaacag
     5101 atgaaacccg acaaagccca ccagacccag cagcagcacg ccggccgtca gcatccagat
     5161 caggttggcg cggatcaggt actgccgttc cagttggcgc gcttcgcgat gcggcgggct
     5221 gatctcccgc accttgcgca cctcggccag cgcggcatcg tactgcccgg cctcggccag
     5281 cgttcgcgca tgattcagaa tctgcgtcag gaatgtgctg agccgttgct gctggcggtt
     5341 gcgaaagtca tgataaaacg cgcggttggc gctcccggag cgcgagggac ctcccagccg
     5401 ccccatccag gcggggctgc cctgcaggaa gttgagcccc agatcgacgc agtggacccg
     5461 ccccgacatg tcggcccggg cgaaggcctg cgccgcgccc cccccggtca gcgggccgac
     5521 cagcggcgtg ttgaccacct ggctgcccag atcgtactgc ccccacgtct cggtgctgcg
     5581 caggttgatc accaccatgc agatgacgtc gcagaagacg gcgtacgccg tgcgcgagtc
     5641 atcgaagaag atcgccggcg gactctcgat cgcgcgctcg tgaagccgtc ccaccagcgc
     5701 cgcgctctcc cagaacggct cgccgctggc gccgtccacc atgtagagcg cccggtcgcg
     5761 aaccaccagc acgtcgtcct tcccgtccgc gttggcatcg acggtcaccg gcgtgaagcg
     5821 cggctcgctc ccgatcgagc ggtgcgcgtc ggcttcggtc ccccccgccg gcggcgccca
     5881 caccggcacg atcgcatcgt cgcgcaccgt caggccgtag aggtagccca ggctgtcggc
     5941 gatcacgatg tcgtcgtaac cgtcgccgtc cagatcgccc agcgcctggc ccagcccgta
     6001 ggtgcggttg gtcgagacca ggttcaacgt gatcgcggcg ggcggcatcg cccgattgaa
     6061 cacggtgacc ttgccctcgc gcgtgacata gacgaagtgc ggcccgcgcc ctccggcgaa
     6121 ccgcgtctga cccaccgacg ggccgcgctc gagaatcccc tgggtcggat ggcggaacag
     6181 ctcgaacaac gactgcgcat ggccgggccc ccgcaactgg cagccgacga gcgtccgttc
     6241 ggatgggttg taaagcagga tcccctcgcg gtagggcgcg atctcgtcct cgccggtcag
     6301 gggaaacgtc gccggcggca gcgtcaggcc gaacccggtt tgctgcgccg gcatctcctg
     6361 ccggccggtc ggtccgtcca atatgaacag tttgccgctg gtgctgggga cgacgatcgt
     6421 gatccggtcg ttgcccagga aattgcccgc gaccggcgac agcagcgtct catcctcgcc
     6481 gagctgctga cgccagatcg gcgcgccatg ggtcggatcg agcacttcga cgacatcgcg
     6541 caggccgacc tggatcaggt ccatccggcc gtccagatcg acgtcgaagg ccagcggatc
     6601 ggagggggca tgggcgaatt cggagtcgcc ttcccagagc agcttcagcg gccgggcctc
     6661 ttgcgccgcg aggtcaaccc caatcgttaa aaaagacaac agacacaaga accgtcgcgt
     6721 cgtccgaacg tgggttggag catccatggt caagtgcatg acattgtgtg gagcggaact
     6781 ctgctgccgg gcatcccggc gccccccatc cacatcgctg gccgccgtca gatcccatcc
     6841 aaagcctgtc aaatcaaatt cacatcctct aagagagagt atagaaattg tggggatgaa
     6901 tcacacgata aaatctagca tgatggaacc cgcgcgtcgc ggccgccccg cgccggctcg
     6961 cttttcccgc cgcgggccga ccgtccaggt gcgtattgac cggcgccgcc cgccgtgcac
     7021 ccatctcccc gcggagcgat cctcacgttg accgcccctc aggccacgtt gccgccccag
     7081 cccgcgccgc gaccgcccga tgcccgcgcc cgcatcgcca cccgccgcgt cacgctgatg
     7141 cacgacttgc tgctgggcat gcgcggcggc gagtgggtcc tcgattcgct cctgaagatt
     7201 ctgccgctgg ccgggatccg cactctgttc tatcggcccc ggttcatcga caccgcaatc
     7261 aactgccggc cgatccatcc cagcccgctg agccgactgc cgggcgtgcg ccgctattac
     7321 cgctggctgc tgccgctgct gccgccgacc gtcgagcggc tgcgcgtccc cgaacgcacc
     7381 gagctggtcc tgacgctcag ccactgcgta gcccatggca tccgggcgcc gcgcggcgtc
     7441 ccccaggtca attattattt cagcccgatg cgctacctgt acgatcagcg cgaggcctac
     7501 cgtcgccatg gcgggctggg cggccgggcg ctcggctggc tggcgccgcg gctggcgcgc
     7561 tgggatttcc aggcggccgc ccgcgccgac caccgctggg cgatctcgca attcgtcgcc
     7621 cggcgcatcg aggacgccta cggtctgacc gcccgcgtga tctatccgcc ggtccgcacc
     7681 gggttcttcg tcccgccgcc gcccgaccgg gccgccgagc gcatccccga atacctcctc
     7741 gtctcggcaa tggtgcccta caagcgcgtc gacctggcca tccgcgccgc caaccggctg
     7801 cgccttccgt tgcgcgtcgt cggcgacggc ccgctgctgc gtcagatgcg ccggctggcc
     7861 ggcccgctgg tcgccgtcga gggccgggtc agccaggcgc ggctgctgga actgtaccag
     7921 acgcgcgcgg ccctgatctt ccccagcgag gaggatttcg gcctggcgcc gctcgaagcg
     7981 ctcgcctgcg gcatgcccgt cctcggcttg cgcgccggtg gattgctcga aacgatgcgc
     8041 gagggccgca ccggcgcctt cttcgaccgg cccaccgtca agtcgctctg cgaggcatgg
     8101 gagcagttcc gccccgcggc ctacgatccg ctccatctgc gcgcccacgc gctggcgttc
     8161 agcgaggagc ggtttcatga ggagatcgcg ctcgccatcg acaccgtcct caatgaatag
     8221 ttgtccttgc cccgccgccg gggcatgatc cgagggcgga tggggcaacc cccatagttg
     8281 gtcttttgcc cggcaagccc ccttcatgca gatcatcggc aacccccgct ttctgaagtc
     8341 cccccgcatc acctgggcgc gctggcggct ggggcacccc gccccgggcc cgatccgcgt
     8401 gcacccggtc ggcggtttca ccttcgagga cggcgcggaa ctgacccagc ggctcacggc
     8461 cgccttcggc gacctggccg gcctgagctg cctcgacctg ggcgccgggc ccggcgagac
     8521 ggtgatcgcc cgccagattc tggacatccc ctgggcccgc ctcgcgtcgg ttgaggcgtt
     8581 tcacccgtat ctgaacaaac tgcgtcagcg gcaggccgcg gcgcggcgcc atgaagtgct
     8641 ggattgccgg attgaacacg tcttcggccg gtttcgcgcg ggggagttcg acctcgccct
     8701 gctgatcgac gtcctggagc atttctcccg ccgcgatgcc gtcgacctgc tcctgcgcct
     8761 ggagcgtttc gtcaatcgcg gcgtcgtcat cttcgcccct gtcggcatcg tcgagcagga
     8821 tgagctggac gccaacgccc tccagcgcca ccgttcccac tggctcgccg aggactggtc
     8881 gcgtctgggc taccaggtgg aggtctatga ggccttccac ggccagctca gtccgcccgc
     8941 aaccgccgcc tgggccatca agtcgtggcg gcgctgaggg ccgcaaccgt cggccttcgg
     9001 gcttgaccgc gcaaccccga acgttccata agggagcatt caggaaccat ctctcgatcg
     9061 attcgagccg ccgaccgccg cggccatctc aaggagttac acgccaccat gcgccgattc
     9121 tgttggaaac cggcggcgcc gttccggctg gggctggtgc tcgcgctggc cgcggccgcc
     9181 gcgaccctcg ccgcccagcc ctccgattcc cccagttccc cctcgctgga ccagctatcc
     9241 gagaaacagg tggaagccct gccggaaggc cccgccgatt ttcagcagat catcgaacgc
     9301 gccaagcgcc gggtcttccc cgcgctcgtc ttcgtcaagc cgatcgtcga ggattacggc
     9361 agcggcgaga agaaaaagca ggaggtcttc ggcagcggcg tcatcatctc gcccgacggc
     9421 ctggtcatca ccaataacca tgtcgtcgag aacgcgatca aggtcaactg cgtcctgttc
     9481 gacaaggatc agctgccggc cgagatcctc gggcgcgatc ccgacaccga tctggcgctg
     9541 ctgcaactgg atgcgccggc ggagaagcgt ccgctgccgg ccgccgagtt cgccgattcg
     9601 gacgcggtcg acgagggcga cttcgtcatg gcgctgggca gcccgttcgg cttcacccgc
     9661 tccatctcgc tgggaatcat ctccaacacg cagcgctatc tcggcttcga gaatgagtat
     9721 cgctacaacc tgtggctgca gaccgatgcc gcgatcaatc cgggcaacag cggcggcccg
     9781 ctcgtcgata tcgagggacg gatcgtcggc atcaacacgc tcggcctgcg gctcgtggcc
     9841 gagaatgtcg gcttttcgat cccggccaat gtcgtccgcg aaatcgccgc ccgcctcgag
     9901 cgcgacggcg ccgtctcgcg cgcctggacc ggtctgcgcc tccagccgct caaggacttc
     9961 aacaccaaca ccttcaccca ggccgaacgc ggcgtcctga tcgccaatgt cgagagcggg
    10021 tcgcccgccg aggcggccgg gctgcaatcg ggcgacctgc tcgtcgccgt cggcgaccac
    10081 ccgatcgacg gcgcctatgt cgaaaacctc cccgccatcc actggcggct ggccgacctg
    10141 ccgaccgatg cgccgaccac cttcacgctg gttcgcaacg gccggaccat cgcggcggcg
    10201 gtccagccgg tgctcaaggg caaggtcgag ggcgagaatc tcgacctgcg ccgctggaac
    10261 atgacgctga aggaaatcaa caaatacaag gaaccccagc tctacttcta tcgtaaggaa
    10321 gggatctacg tgcagggcgt gcgcaatccg ggcaatgcct cggcggccgg cattcagcgc
    10381 aacgacatcc tgctctcgat cgagcgtcag ccggtcgact cgctggcgac cgcgcgccag
    10441 ctctacgagt cgattctggg ggacgagacg cgcgagaaaa aagtcctgct cgaagtgctg
    10501 cgcgggggat tgcccaagct gatcgtcctc gactacacca aagactatga aaaggcggac
    10561 tgactcatga acggctcatt cctcgcccgc gcgtcccgcg ccgtgcggtt gctcgcgctg
    10621 ctcggccttg cctgcctggc gcgcccgtgc ctggcccagc cggcggcggc cgcgagcgcc
    10681 gacgccgccc ccacgaccgg gccgctcacg ctccagcagg cggagtcatt ccttgagcac
    10741 ttcgccgtca ttgaagtctt tttcaagcgc agcgaagaag acaaggatgc cgttcacccg
    10801 gagtatgaat cctacatcag ccgcaagctc ccgatgcggc tgatcggcat cgcgctcgac
    10861 gccgagcacg tcgtgacgat cgacaccgac ctggacgaaa aatggatcga ccggatccag
    10921 gtgaccgaca tgcggggtcg gagcgcgccg gccgagcgcg cggcgttgct gctcgatgcc
    10981 cccgcgctcg tctttcgcgt cgccgacccg accggcctga cgctgcgccc gccccagttc
    11041 accgaccccg gccccgccgc gcccgatggc ctgcagcaac tggtcgtcac gcgccacggc
    11101 agcaactaca tgcttcagct ttcccccgcc cagccgctgg tccaattgac gccggagtat
    11161 cagctccagc gggtgctctc caccgcggtc tatgaccccg acgccagcag ccgcgggatg
    11221 atggacgact ggagcggctg gtcgtcggac tttgaaatca acaaggtccc ggtgctgctg
    11281 ctggatcagc ggcggcggcc cgtcggcctc gccttccgcg cctatctgcc ctacggtgag
    11341 agtgcagagg agggtctgcg ccccgaccag cgcgaacgcg tcgcctgggc ccaatacgcc
    11401 gccgcgcagg atcaactgac gaccgcggcc ggccgctggg tgatggagac ccacatcacg
    11461 ttccgccaga aagccgagga aggcggcggc accttcctcg gcctgccgct gcccaatgtg
    11521 gactcgatca tggggggcca ggtcaacgtt cccgatgagt ggtatgttta cggtttcccc
    11581 attgcgcccg atctgctctt cgtccccacc cgcatccagc gcgaattcgc caaggtgatc
    11641 gaccggatcg aggtccgcga cggcgaccgc acgattccgg ggcgctttta cggcgcctat
    11701 gaggacatcg ccgccttcct gatccggctc gatgaacccc tctcggtcgc cccggccgaa
    11761 atcttctcca gccagccggt gcgcatgatc cggccgatgc tctccatcta tcccgagcgg
    11821 cgctatggtc gcgagtatct cgatgtcggc tatgcccgtc tgctcagccg gctcaacggc
    11881 tatcgcgacg tgctggagtt tgtgccggcc tacgaggcgc gcgtcggcgc cctctacacc
    11941 gacctcgacg gcaacctgca gggggtcacc ctcgagcagc gcaaggagga cgaggacaag
    12001 gagcgattcc ggcgcgccgg cgggttcccc tatttcggcg gcggcgaaac cttctaccgc
    12061 ttctactcaa ttcccgagct cgccccgtac ttcaatgcgc ccaacgatca tctcgatccg
    12121 acgatcgtct cgctctccga ggaggaagag gagcgcaaga tgtggctcgg cgtcgagttc
    12181 gatccgatga cgcaggagct ggccaagacg ctcgacgtgc agccccagac gaaggacggc
    12241 acgatcggcc tgtgggtcac gcgtgtctat cccggctcac cggcggcgcg cctcggcctg
    12301 gaacgcggtt cgattctgct cgagctgcgc gaggagggcg aagcccagcc ggttgagctc
    12361 aaggccgccg acacgtcgat gtacgacttc gacttttccg atgtggaact gcccgagggc
    12421 atggagaacc tggggatcag cctgcccagg gccaaaccgt ggaagaatcg gcgcaacatg
    12481 ctgacgcgcc tgctcgaggt catcggcgag ggccggaccg tccacctgcg gtacctcgac
    12541 ccgcagcggg cggagcacag cgtgacgttc gccgtcgaaa aggcgccgca tgatttcgcc
    12601 agcgcgccca agtacaagga cgaggagatc ggcctgaccg tcaaggagct gacctatgag
    12661 gtgcgcgaag ccttgatgct gcctcccgat gcccgtcacc tggtcgtctc cagcgtggag
    12721 gaggggagcc cggccgccgt cgcgcgcgtc gaactttacg acctgatcat cgcgatcaac
    12781 gatcagccgg tgcccgatct ggagacgttc aagcggatcg cccaggacgc ccgcgccgag
    12841 cgcgaagcca gcggccgcgc cacgctgcgc gtcacgcttc agcaactcga caaaacccgg
    12901 atcgtcgacc tgacgctgga gtgacccccg cggacctcaa cgcaccggct ggaccgtggc
    12961 ctcggagcgg ccggcttcgg cgggcggctg cagcgcgctc cacagcccga acgccaccgc
    13021 gtagagcgcg acggcaagca gcagcacgta cagcgtgatc cggccgccca ggttgccgcg
    13081 ctcgcgctgc atccgccgtt cgcgctcacg ccacagcgcg tcgatgtagg ggttggcgtc
    13141 gcggtaggcc tgccgcgtgc tttccgcgct ctcgatccga tgcgggaagg cttcgtagaa
    13201 gtgccggtcg tattccatcc gccgctcggg ccgatgcagc acctcgtagg ccgcgttgat
    13261 ggcggccatg cgctgctccg cgcgcgcgtc gccgccgtgg cggtcgggat ggtgccggcg
    13321 caccagcagg cggtaggtct gcttgatttc gtgaaacgtc gccgtcggtt tcagtccgag
    13381 cgtggcgtaa tgatcgggca ggggcatctt cgtcggcggg ggcggcggga atcggtcagc
    13441 gtgagcagcc gtcgtacgct ctgaccggat caaggtagca caaccggccg cgcggctcca
    13501 gtcgccgcgt gcgtgggttc cgatcccgtc cgccgccggt cagtccgtca cccgaatcgt
    13561 ccggtggcag cgcgggcatt cgacgcgctc ggcgctgaac gtcgggctga gcgagacgtg
    13621 gccgccgcag cggcaggcga tcgtctccca tcccgatccg ccgcggcggt ggatctgctc
    13681 cgtctcggcg gcggcgcccg ccggcacggc gacttccgct tcggccttga gcgccgggcg
    13741 ggccgcgttg ctgaaatgcg tccgcccgca gcgcgggcac tggattttcg gctcgctgta
    13801 gttcggcggc agcttgatcg tcaacccgca tgcgcacggc aggatcagca gccccgccat
    13861 cacgtccatc aggcgcagcg cctcgcgcgc gcgcgccacg gcgtcgcgtt gttcctcggc
    13921 gggcgcagcc gcgcggcggg ggacccgctc cccttcctcg gcgcgcgtgc gctcgccgaa
    13981 caaggccccg cgctcgcccg cgcgctcgcg ccaggcccgc tcatactcgg cgaagcccgc
    14041 gccgccggcc atcgcgcgca gaatcgcgat ccggcggtcg gtcgacggat gggtcgcaaa
    14101 caggccgccg ccgcccattg cccgcagcgg gttgacgatg aacatcggcg ccaccgcccg
    14161 gttctgtttg ccgagcagcc gcccccccgc gcggccgctg atcttctcca gcgccgaggc
    14221 gagcccctcg ggaaaccgcg taaaccgggc ggccgaggca tcggccagat actcgcgccg
    14281 gcgcgagcag gcaaagtaaa gcaactgcgc gcagagcggg gccaggatcg ccagcgccag
    14341 cgcgacaagc agcatgatcg cctgcgcctg gttgcccccg tcgcggctgt cgcgtccccg
    14401 ccggctcgaa tggcgcccgc catggaggaa ggcgcgaaga aaaacatccg aaatcagcgc
    14461 gatgctgccc atcatcaccg cggccagcgt caggaaccgg atgtcgagat tgcgcacatg
    14521 cccgatctca tgcgccacca cgccctgcaa ctcgtcgcgg ttgagcttgc gcagcagacc
    14581 gatcgtgacg gccaccgccg cccgctccgg attgcggccc acggcaaacg cgttcagact
    14641 gtcggtgtcg agcacgtaga cgtcgggtcg ccggggaaga cccgccgcca gcgtcatctc
    14701 ctcgacgacg ttgaacaggc ggcgcgcctc ggccggagtc tcaagtttgc gcgcccccat
    14761 gctcttgagc aggatcgcct cgcccgccgt gagcgacacg agcgtcatca cgccgaagac
    14821 cgccagcgcg gccagcacgc cgaagacgcc gcccgtcgcc ggatccggat cgatgctcat
    14881 gccgatcgct ccgcccagcg ccgccagcag cagggccatc aaggccagca gcaggcgcga
    14941 gcggcgccga tttgcggcaa tcgcttccca catggcggcc tagaatttca cctgcggcac
    15001 ggcccgttcg ctcttgtctt ccagctcaaa gaaggcctct tcgcggaact ggaacatgcc
    15061 ggccacgaga ttggcgggga tctgctggat ccgggtgttg tactgcatga cctgttcgtt
    15121 gtagaactgg cgcgagaagc caatcttgtt ttcggtcgac gtcagttcct cctgcaacgc
    15181 cagaaaattc tgattcgcct tcaggtcggg gtattgctcc acgaccatca gcaggcggcc
    15241 cagcgcgccg ctcagttgcg attcggcctg tgcctgctcg ccgacgccgt gggccgagac
    15301 ggcggcggcg cgcgcccgca tcacttcctc cagcgtctgg cgctcgtgtc ccgcgtagcc
    15361 cttgaccgtc tcgaccaggt ttggaatcag gtcgtggcgc cgcttgagct gcacgtcgat
    15421 ctgcgcccag gcttccttga cctgattgcg caggcgcacc agaccgttgt aggtcgcgat
    15481 gaaccacagg atgacgagca gcaccaggcc gccgatgaca agcaatgcga tgagtgcgcc
    15541 gcccatgagg attagccttc ctgtcgggga ggttcggccg cggaggggag gaaattccgc
    15601 ggaccgcgct cggagcgaaa ttccgtcgcc agcacgtcgc ccagaaagtc cgccagcgcg
    15661 ccggcctcgt cgccgccggc gtgctcgatc agccggcgga tctcgccggc gtcgaaccac
    15721 aagccgtggc cgcgcgggca gtggtccagc tcaagggggg gctccgccgc cagcgtcagc
    15781 ccccggcagc gccacccgca gcggggacag cgccgggcgc cacgccgacc gcgccggccg
    15841 gcgttcaacg ccgccgtcag cgggccgggt ggcgccccgg ccagatcgca aatctgctcc
    15901 agctcgccgc catcgagcca cgttccgccg cattgggggc agtgatcgat ctcgacgccg
    15961 tgcagttcga ggacgagcat cggcgcgccg caatgcgggc acatcagcgg cgtctctccg
    16021 cacgatcggg ctggcggcaa cccatgcctg gcaacctcaa gtcaagctcc gcttattgag
    16081 agccaatggc tggagactgt caagttcccg ccacgcgcga tcggccgtgt tctttgtccc
    16141 ttatatccct tgtgtctctt gtgtcctttc tgcccttcca tccctacttc actctccccc
    16201 tcacctgccg tgtttcgttg attccgaacc actgtccggt ccgatcaccg gcattcaacc
    16261 cccgccgggg tatcgtcgat aagagaagca aatggcttgg ccgaagtgcg tccggcgggg
    16321 cgcccggcgc cgcccgcgcc acgttcggaa cggttgtcat ggcttcctcc tttctgcttt
    16381 ctctgtcgtc ggcgcgcgcg gcgaccgatc gcgccgcccg gccggcccat ccccaccggc
    16441 tgcggctgat cgtcaccgtc ccctcggtgc tgatcctctt cacgctgggc tacggcctgg
    16501 tctcttacca gacgttctca agtcactggg aagtgctcga atcactcggc gcgggcgagg
    16561 tggccgccac gctgctgcgc gtgcatctga ccgcgatgct ggtcctctcg ctgctggcgg
    16621 gcctgatcgg catcgcgctg accgatatga tcctgcgccc gctgcgcagc ctgatggaga
    16681 cggcgcggcg ggtgggggag ggccagctcg acgcgaccgc cccgagcctg cctggcgcgt
    16741 cggagttcgc cgagctgagc cagagcttca accgcatgct gcaggacgtc aacgaatcga
    16801 tcgcgcggcg caaccgctgc ctggtcgaca cgatcccgat cggcgtcctc accaccaacc
    16861 tggaaggggt cgtcacgacg atcaacccgg ccggcgcccg gctgctggcg ctcgcgccgg
    16921 agcaggtgcg cgggcggcgg ctggccgacc tggcgcaggc ggtcccgccc gatgcccgcg
    16981 agctgatcga gcggttccag accatcctgg aggggcctga gccgactttt cagggctcgc
    17041 ttgagctgcc cgctgatggc ccggccggcg tccgccgcat gaccgtcgcc agttcctcgc
    17101 tgcgcgacgc cgacggcagc ccgtggggct tgctgttcag cttccgcgac gccggcgccg
    17161 tcgaaacgct ctccgctcac ctgagccata ccgatcatct ggccgcactc ggcacgctgg
    17221 cgctgggtct ggcccacgaa ctgcgcaacc cgctggccgc cgtcaagggc ctgagccagc
    17281 tcctgcaaag cgaacgcgat ctgcccgact caacccgcaa gtacctggag cggatgaccc
    17341 gtgaggtgga ccgggtggat ctgttcgtca agcggctgct cgaactgggc gagcggtcgg
    17401 cgcaggggcc gacggcgtgc gacctggggc ggttgttgac cgaggccgac cggcgcgcgc
    17461 gcgccgaagc ccgcgaggcc gcggcgcatg ttgcgcgcga tctcgagctc cagccgctgc
    17521 cgccgctgat ggtcgagcgc gaccgattgg agcaggcgct gttccagatt cttctcaatg
    17581 cctatcaatg cacgccgccg ggggggcgca ttcgggcgac gacctcggcc ggcttcgacg
    17641 acggcggccg gctgcgcttc cgcatcgccg tccacaatac cggctcgacg atcgcaccgg
    17701 ccgaccgccg ccgcgtcttc gagccgttct tcaccacgcg tgatcaggcc accggccttg
    17761 gcttgaccta cgcgcatcag atcgtcagcc agaacggcgg cgagctgacg ctcgaagtcg
    17821 aagaggacgg cgtcaggttc atcctccagt ttggtgaaga gcggcgcgcc atctcgtccg
    17881 aaaccgaaac cgcgtccaat ccgcgggaag gcgaacagcc atgagtgcga cattccacga
    17941 tcccagccgc ccagccatgg gcgcccccgg aacacccggc ccgaatccgc tcgccggcga
    18001 tccgctcgaa ctgggccgcg ccgacggcta ccgcgtcctg atcgtcgacg acgaggacac
    18061 gatccgtttc attctgcgcg aactgctcga gcgcgagggc tgcatcgtcg acgaagccga
    18121 aaacggcgtc gaggcgctca acatggcccg gcgcaacggc tatgacctct atctgctcga
    18181 catgaaaatg cccgagatgg acggcctggc cgcgctgcgc cagatccgcc ggatctatcc
    18241 cgacgccctg gccgtgatga ttaccgcgtt cggctccaac cagctcgcgc tggattgcct
    18301 gaaggcgggg gcctacgact atttcaccaa gccgttcaac atcgaggagc tgcgcatcct
    18361 gctgctgcgg gccctggaga agcaccaccg ggacctcgag gacttcttcc tcgacg
//
LOCUS       DAOPCO010000047        18293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3147_length_18293_cov_4.849709, whole genome shotgun sequence.
ACCESSION   DAOPCO010000047 DAOPCO010000000
VERSION     DAOPCO010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 18293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18293
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3147_length_18293_cov_4.849709"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1027
                     /locus_tag="PLS90_05495"
     CDS             <1..1027
                     /locus_tag="PLS90_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01891.1"
                     /translation="IARSSGPGETLWENLEFKAEGDSCSVFVQVSIGPSDNPNFKFQI
                     QRFRNCLFRDAIKGLDPGAGAIYVFDEAFQSSPVDQGGLVYVENCQFQNTMCAVRVRA
                     TADHDSTPTQVFMSDCTMNLLIPPEVTATSRGGLRVSGSSGHLTVTNCTFISEGASEE
                     ERPARFVMAGNTDFYPQPWADDFGSLTMERCTILGAHICGIRIMGSGDVNLDHIDVHA
                     YGNYTEGAGILLAEDGDFFGYNGSLNLTNSNILCDNTFSLKGLAENTAVINATYNNVP
                     NGIDGSWPTSSTNFSVDPQWANYPTDLTVGNPVLFSAGNDGFDIGSVELSQGGPLPNQ
                     AAHWLVY"
     assembly_gap    1312..1411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1412..1694)
                     /locus_tag="PLS90_05500"
     CDS             complement(<1412..1694)
                     /locus_tag="PLS90_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01892.1"
                     /translation="MVLGDADNDGRMDVFVSRPREGSTGESCTAYGVGAGGAVAALWS
                     LDAPDGLAADAVAVADAEGAGRNKALVRFTGHGAPGAQIVTQGAGITRDL"
     gene            complement(1846..2808)
                     /locus_tag="PLS90_05505"
     CDS             complement(1846..2808)
                     /locus_tag="PLS90_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01893.1"
                     /translation="MKRIAIVGGETHIHEVTRLAGQHLTIVGAVVRESQKAKAAEDFK
                     CPLFDSEAALYEQAKPDVVAVANQNDLKGACVLRALRAGCDVVADKPLTISMDEQREI
                     EAFLRAHPERRLLVLLTLRGQGMWAEVRNQVRDGTIGRPAFCHVRMAVQLMRELRPDW
                     FLDVNRSGGLFLDLLIHGIDQVEWALDSKIVAMTATMGNLGYPEDVNLRDHAAVYCEL
                     ASGATAIIEGQRMLPEPAGADYRMLVAGTQGYLDLDFGESTLHVTDREAKRRAITEFP
                     ETQSVLADWLAGGDLVDQESSLRANRLSILATQSAEEQRRIVLD"
     gene            complement(2887..3753)
                     /locus_tag="PLS90_05510"
     CDS             complement(2887..3753)
                     /locus_tag="PLS90_05510"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPK01894.1"
                     /translation="MLDHFLLSLRARKENERDGDAPFAGLFTLEQDETTGPRTVRFEP
                     KQRHGLDRLRLTDGIVAELRRAILSEELKPGDRLPPESELVRLFGVGRSTIREAMRAM
                     AQLGLVRIQQGRGTFVQPNAKEVLLSANPGRPNLVNLPDDQIHEVRSIIEGEMAALAA
                     ERAREGDLERMATLIDRMRREANEPQKFLVSDMNFHLALAHAARNQLLEQFLFFAYEL
                     FEQVTGPVLQAASARERAIWFYQELWDAVSQKDCASARHLMLDYLLLLQEEKAKGETG
                     GGRTAVSRRASG"
     gene            4138..5418
                     /locus_tag="PLS90_05515"
     CDS             4138..5418
                     /locus_tag="PLS90_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01895.1"
                     /translation="MRFFQPLLIILAAVGLLLPSGSLSAATDAEYIAQFINYQSSFGQ
                     INDPVPTLDEYFAETIPRFDEQYVDNIQVWVDQIADQQNILGEMGWTSSAVSTSLNDM
                     YAVTGDLKYLRASLEITSVSLQVRDDKIGRTTFWGESAPGWSTPRYALRWVIHPVHTA
                     MITWGMLESLWLAQANPVAMAELGDEFDSILAQAAESIDWHNRQWVEGPASDEGYYIY
                     KDNEPDREGQRLPFNRQSTMGRSLWMLYKLGGNPEHRDKAIKLATFLKRRLHLFLTPD
                     TNEGVWFWEYTLPNSPRNNPYPWSQLWRVGTGDDLSHGSLSMEFYVLMANDGQVFTHE
                     DMQALARTVTYGFARLDNGILFGNVAGSPDLHDPSYVANTGYWLRLTPYNPEVYDRIA
                     AFMLKYQQEPRGLDLAKLVRWKMPSAVPQSWLYE"
     assembly_gap    5497..5596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5888..7309)
                     /locus_tag="PLS90_05520"
     CDS             complement(5888..7309)
                     /locus_tag="PLS90_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01896.1"
                     /translation="MLLLTTEPLPVGGLPATGAGLRAWGLARGLRQAGLDVQLLMPAR
                     AAATFAPAAAEPKGSGGWPEWVGTFERANIAAALAEHDPDVIVLQHWGLARDLGEPDR
                     PLAIDLAGPHLLERTYWGSTTHEADLEEKLAALRRADFLTASGERQRLYFLPYLALAG
                     WPVAATDVLPVIPFSFEPLMDRPRVRPDRLIYGGFFLPWQDPTRALETALDALDATGR
                     GELLFVGGAHPTFDVSRGQFEALLDRLGRHPRVRLVPAMGFEAYARLLAEGGVALDLL
                     ARNPEREIAFTTRTVVYLAAGLPVIHNHYSELGALIARAGAGWALDPDDAEGLGALLR
                     RVLSGEEPIATRAEAALEVVRGELNWERTIEPLARFCRAPAVRADKQAARLAFEERHR
                     HLRRLEGELAETRGRLETLLGKCWVRWGLAATAGDTWLRWPLAVLAGVVGLALIPIFV
                     VNELIGRAFTSRVAREPGEDGGK"
     gene            7512..8327
                     /gene="cysE"
                     /locus_tag="PLS90_05525"
     CDS             7512..8327
                     /gene="cysE"
                     /locus_tag="PLS90_05525"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPK01897.1"
                     /translation="MNQPNDPIWTTIVEEVEQAARSEVMLASFLHATILNHERFEDAL
                     SFHLASKLGSATIHTMTLREVIYQALNADPAIGEAARADIHAVRDRDPACRYYSTPLL
                     YFKGYSALQAHRIAHWLWHRGRIQLALYLQSRVSEIFAVDIHPAARIGKGIMLDHATG
                     IVIGETAAIDDNVSMLHGVTLGGTGKERGDRHPKIRQGCLIGVGAKVLGNIVVGEGSI
                     VGAGSVVLHPVPPHSRVVGVPARVIGRTHGEDPALVMDQFVDRDPNDPENGAQ"
     gene            8324..9856
                     /locus_tag="PLS90_05530"
     CDS             8324..9856
                     /locus_tag="PLS90_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPK01898.1"
                     /translation="MKLVTARQMREIDRITIAERGVPAAELMERAGQAVAREALERLH
                     PDRVAIVTGKGNNAGDGFVAARALHQANVRTTLYLLRPPDELQGEALAAWRAVPAPIE
                     RIVVQSPADLARLGEHDLIIDAIFGTGIRGPVQEPWASYIAAVNAAGANILSIDIPSG
                     LPGDPAEEPGPHVRSQLTVTIGLPKAGMLLGAGLRATGQVVVADIGFPRELLEDPAIT
                     LNLMTAEEARALLPPRDPAGHKGTFGRALILGGSEGMTGAAVLAARAAARSGAGLIYS
                     AYPAPLGPIMESHLIEPVKLPLEGDERWFTPRHLEAVLEHAERMDAVALGPGIGTRKP
                     TARFVQAVLENVKAPMVVDADGLNLIAKRRVDLRRRPGPTVLTPHPAEAARLLELETG
                     EVVRRRLDVMTEFAARHNVVVVLKGAQTLTIAPDGQRTVNPTGNSGLAKGGSGDVLTG
                     LIAGLLAQGLSALDAARLGVFLHGLSADVVAERISVRAMLPSDLLDAFGTAFQRLERN
                     AP"
     gene            complement(10134..14423)
                     /locus_tag="PLS90_05535"
     CDS             complement(10134..14423)
                     /locus_tag="PLS90_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01899.1"
                     /translation="MKSISLVVLTLTVLVSVLGCQSLPRRREAAAIDSAARYRPLMPS
                     FALEPGQTRRQSADYKRQGKPDLAWLWFYNHRVDQTGVVPIAYREEALDHSRKYNQRR
                     PKDVGAAWTYLGPGGQVDAPPNGRITDLVVDPLNEQILYAATAGGGLWKTTDQGLNWY
                     NLTDGKVPTLGVGSIAMDPVQPSTLYIGLGEGNKGHQNGMDPLGTGIYKSTDGGQNWA
                     LLPGTGNGVMAFIGDIKLVNSDTILAAAWGGHDQAGRGLFRSTDGGQSWVKRIDEVAV
                     SISIDPTNRSRIAVSTWRGAGATAEVVLYTSADGGLTFNPAVTTPDRSGGRRAELARD
                     PSNPNRLYALVASTNSSIKGIWTSADGGQDWTELPKAGTSAAEQMNYNNCIAVSPADP
                     NRIYLGTNLRSYRSTDGGQSWAAMTDWDPENNAGLPSIHADHHAIAFGASANTIYIGT
                     DGGCFMSTDGGNSWIERNAGLNCTQIYRIDNHPTMANAVIMGTQDNDKYVRRPDGTWR
                     HWPNEFGDCMEVAVDPLDPEGDGFYGVNYFGNLMKYTGNGSQSWHDYLRTYNGFPDAG
                     SMPNGIPQNEQGAWVTPFLLDPQQPRTAYVVLNHIYRTQFTPGAVPQWEKLLEMDQQF
                     SHTFEHADLSSGLRNRQIYVATNRFDQTLGAYVPGLLRMDIGDGSGAISEPRELDPPR
                     QGILGRIKCDPAQNDWVWLCYTDYGQGKDAPNGRIYLSTDRGQTWTDKTGNLPRQLPV
                     SAIFIDPLNPSTVIVGTDLGCYRTDDHGVTWYEFNAGLPMTVVTDLAYFAPQRLLRAG
                     TFGRGMWETGLDGAGVADIAVQPASVNFSLDTRRKTAGTTGLQPGDAALDAPPPRPVI
                     PEALRDAKPIQLPKGQLGILNDSFEVGDTLAAGWYIPATDDPHWWFDDLRKNSGLRAL
                     WCAAGGSLGRDPQIDSYPANFMTSLTFGPFSLADATAASFQFWYDLDTDAWNGTTGDL
                     FYVLVYRNQTEYGYYVASGLAANQNFTVDLSNVSGLGNILGNELCYVQFQFVSDANYN
                     LARYGVWIDDVVLNKTTAALGTPTGLTASTNLTDRVTLSWNPLAGALAYYVYRNTSNT
                     TAGASIIAGPLSGTTFDDTTAAPGVDYFYYVLGVNSTEISSFSNSVLGRRKAPIQSPV
                     ALATIHLTDRVEITWNDVGASSYFVSRSEGVNDLAQAKSITPSMTETFATDRNVRAGV
                     NYYYFVRGLGSEPVWSEGVLGRVGSVALLAGPGGVAASSNLLDHVRVTWNANVPQATD
                     YQVYRTTGVDNPSAALPISPWIAEGMFDDHTALPGITYFYYVKARNAQTGGHVSATAT
                     GRLAPTVLAPQTFTVRNIGNASFTIYGVVVQSGSTWLGVVTADSLPRKLAPGQELPVT
                     VIADPTGLGDGRFSDRVLVVNDVVGRNPMLVDVTLSKIQTGVELEVWEPYE"
     gene            complement(14638..15615)
                     /locus_tag="PLS90_05540"
     CDS             complement(14638..15615)
                     /locus_tag="PLS90_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01900.1"
                     /translation="MIQFVFLSDFFRNNSKRGVWIDDVVITKTVNDPLQAPGGVTATT
                     NLTDRVTINWNTVAGATEYRVQRNTTNNAAQATPLGNWAGATSYDDLTALAETDYYYF
                     VQARNGGQTSPLSSGVLGRRAAAPPLDPPTGVIATTQLADRVTVQWNPVAQATEYQVL
                     RNQGVDNAGQANDLSGWIGATSYDDLVAVPGVDYYYYVRGRNAQSTGNLSAGALGRIP
                     PPPIAPQDIVIKNIGDATLEITGITFQNNSPWLKVTPPSAYPIKISPNQAQTFTVVAN
                     PTGLGQGEHGDRLVIDNNVPGKDPYPTGVEITLTIEQNRVELRLWKAYE"
     gene            complement(15649..>18293)
                     /locus_tag="PLS90_05545"
     CDS             complement(15649..>18293)
                     /locus_tag="PLS90_05545"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01901.1"
                     /translation="GRIIDLAVHPQNEQIIYAATAGGGVWKTINQGQSWTNLTDGKIP
                     SLGCGSLVMQPGNPETLLLGLGEGNKGFGNDVDPLGTGIYRTTDGGQTWNLVPGTGNG
                     TMQFVGDIRFVDANTILAAAWGGYQLAGRGLWRSTDGGQNWTRIVDEPAVSISVDPTN
                     SNNVALTTWVVETGVGTTTRFYYSTNGGASFNPATYPNATGCFRAELARDPTTPTKLY
                     ALVATGDSKIKGIWSSTNGGQNWSALTTNGIPAGDSGPGQMNYNNCIAVSPNDSNRLY
                     FGSNLRSYWSQNGGLNWTPMTDWNADDGDGIPYIHADHHVIAFGSTGNTIYMGTDGGF
                     FMSSDGGATWIERNEGLDCTQIYRIANHPTIANALIMGTQDNDKYVRRPDGTWRHWPN
                     SFGDCMEVAVDTLDAQGDGFYGMNYFGTTMRYTGNGSQSWNDFLRTYTGYPEINSTPN
                     GIPDNEQGAWVAPLLVDPQHPRSVWVILQNVYRQQFTPGAVAQWQQLATINANYVPGG
                     TFEIADLSSGTNNRQLYAALNRFDQVQQVFDPGILRMDIGDGLGAASNAVFPTPPRPG
                     ILGTLKCDPVANDSIWVTYTDYGQNKANPAGRVYMSPNKGQSWENRTGNLPSHLPVSA
                     IFVDPEAPATLVVGTDLGCYRSDDGGATWYEYNDGLPNAVVTDLDYFPPERKLRVGTF
                     GRGLWETPLAGGSPDIEVTPESLAFTFSDGKQAAGRMGCGKAAKDGRGPVMTEALRKA
                     APIDQPKAEMQILNETFDTGALANGWFINPFEDPYWWFDDFRSNSGTFSFWCARGGFF
                     GVDPEFSTYPEGVITYLTFGPFSLADATEATLQFWYDVDIDFAVLGEEFRVYAYRQAD
                     PGSAGYYYFTGTLAGTFEKSLKTD"
BASE COUNT         2918 a         6537 c         5472 g         3166 t
ORIGIN      
        1 catcgcccgc agctcgggcc cgggtgaaac cctctgggag aatctggagt tcaaggccga
       61 gggcgatagc tgcagcgtgt tcgtccaggt ttcgatcggc cccagcgaca acccgaactt
      121 caagttccag attcagcgct tccgcaactg cctgttccgc gacgcgatca agggcctcga
      181 tccgggcgcc ggcgcgatct acgtcttcga cgaggcgttc cagtccagcc cggtcgatca
      241 gggcggcctg gtctatgtcg agaactgcca gttccaaaac acgatgtgcg cggtccgcgt
      301 ccgcgccacc gccgaccatg attcgacgcc gacgcaggtc ttcatgtccg actgcacaat
      361 gaatctgctg attccgcccg aggtgacggc gacaagccgc ggcggcctgc gcgtcagcgg
      421 ctcctccggc cacctgacgg tgaccaactg cacgttcatt tccgaggggg cgtcggaaga
      481 ggagcggccg gcgcggttcg tcatggccgg caacaccgat ttctatccgc agccctgggc
      541 agatgatttc ggctcgctga caatggaacg ctgcaccatc ctgggcgctc acatctgcgg
      601 cattcgaatc atgggcagcg gcgacgtcaa cctcgaccac attgatgtcc atgcctacgg
      661 caattatacc gagggcgccg ggatcctgtt ggcggaagat ggcgacttct tcggctacaa
      721 cggttcgctc aacctgacca acagcaacat cctctgcgac aacaccttct cgctcaaggg
      781 cctggccgag aacaccgccg tgatcaacgc cacctataac aacgtgccca acggcatcga
      841 cggcagttgg ccgacctcct ccaccaactt ctccgtcgat ccgcagtggg ccaattatcc
      901 gaccgacctg accgtgggca atccggtcct gttctccgcc ggcaacgacg gcttcgacat
      961 cggctcggtc gaactgagcc agggcgggcc gctccccaac caggcggccc actggttggt
     1021 ctactgagat tccgttcggg acttggtatg aatcagacgg tcgtctgtcg gtcccagcct
     1081 ggactgacag acgaccgttt tttatgaaag ccgtcccctt gatgatggat ccgtccctgg
     1141 tggctcgtcc tcgccctcgc catcgccccc aatggcctgc ttccagattc ggctctcggc
     1201 tctcgtccct cgtcgtcggc tctccaaaac cggccactgg gatagggata cgtcctgcag
     1261 atctcgcgtg atccccgccc cctgcgtgac gatctgcgcg ccgggcgcgc cnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcagatctc gcgtgatccc cgccccctgc
     1441 gtgacgatct gcgcgccggg cgcgccgtga cctgtaaacc gcaccagcgc cttgttccgg
     1501 cccgcgccct ccgcgtccgc caccgcgacc gcgtcagccg ccagtccatc gggcgcgtcc
     1561 agcgaccaga gcgcggccac cgcgcccccg gccccgaccc cgtatgccgt gcacgactcg
     1621 ccggtcgagc cctccctggg ccggctgacg aacacatcca tccggccgtc attgtcggcg
     1681 tcgcccagaa ccaccgtctc cagcccgccc gccgcgcccg tggcgaaatg tagccaactc
     1741 ttttttgcgc cggggaacgc gagcgctgaa caacatgcgg gcgggatcgg cccgaaccgc
     1801 gacctgatcc cgtcgccgca agggtgccca tccgcgccgg gccggtcagt ccagcacgat
     1861 ccgccgctgc tcctcggccg actgcgtcgc caggatcgac agacggtttg cgcgcagcga
     1921 cgactcctga tcaaccaggt cgccccccgc cagccagtcg gccaggaccg actgcgtttc
     1981 ggggaactcg gtgatcgcgc gccgcttcgc ctcccggtcg gtcacgtgca gcgtgctctc
     2041 gccgaaatcc agatccaggt aaccctgcgt gccggccacc agcatccggt aatcggcgcc
     2101 cgccggctcg gggagcatgc gctgcccctc gatgatcgcc gtggcgccgc tggccagctc
     2161 gcaatacacc gccgcgtgat cgcgcagatt cacgtcctcc ggatagccca ggttgcccat
     2221 cgtcgcggtc atcgcgacga tcttggagtc cagtgcccac tccacctggt cgatgccgtg
     2281 gatcagcagg tccagaaaca ggccccccga gcggttgacg tccaggaacc agtcgggccg
     2341 cagttcgcgc atcagttgca ccgccatccg gacatggcag aacgctgggc ggccgatcgt
     2401 cccgtcccgc acctgattgc gcacctcggc ccacatcccc tggccgcgca gcgtcagcag
     2461 caccagcagg cgccgttcgg ggtgcgcgcg caggaaggcc tcgatttcgc gctgctcgtc
     2521 catcgagatc gttagcggct tgtccgcgac gacgtcgcaa ccggcccgca gggcgcgcag
     2581 tacgcaggcc cccttcaggt cattctgatt ggccaccgcg acgacgtcgg gcttggcttg
     2641 ttcgtacagg gccgcttcgg aatcgaacaa cgggcatttg aagtcctcgg cggccttggc
     2701 cttctgcgac tcgcggacga cggcgccgac gatcgtcagg tgctggccgg ccaggcgggt
     2761 gacttcatgg atatgggttt cgccgccgac gatggcgatg cgtttcatgg gctcctcctg
     2821 caaatgataa gatgactgat gttttagccg aaatccccgc tcccctcaac cgtcttccct
     2881 cggcgcctaa cccgacgccc gccggctcac cgccgtccgc ccgccccccg tctccccctt
     2941 cgccttctcc tcctgcagca gcaacaggta gtccagcatc agatgacgcg ccgacgcgca
     3001 gtccttctgg ctgaccgcgt cccagagctc ctggtagaac cagatcgccc gctcgcgcgc
     3061 cgatgccgcc tgcaacaccg gtcccgtcac ctgttcgaac agctcgtagg cgaaaaacag
     3121 gaattgctcc agcaactggt tgcgcgccgc atgcgccagc gccaggtgga aattcatgtc
     3181 gctgaccaga aacttctgcg gctcgttggc ttcgcgccgc atccggtcga tcagcgtcgc
     3241 catccgctcc aggtccccct cgcgcgcccg ttccgcggcc agcgccgcca tctccccctc
     3301 gatgatcgac cgcacctcgt ggatctggtc gtcgggcaga ttgaccaggt tgggccggcc
     3361 gggattagcg ctcagcaaca cttccttggc attgggctgc acgaacgtcc cacggccctg
     3421 ttgaatccgc accagcccca gctgcgccat cgcgcgcatc gcctcgcgga tcgtcgagcg
     3481 ccccacgccg aacagccgca ccagttcgct ctcgggcggc agccggtccc ccggcttgag
     3541 ctcctcgctc aggatcgccc ggcgcagctc ggccacgatc ccgtcggtca gccgcagccg
     3601 gtccagcccg tggcgctgct tcggctcgaa ccggaccgtg cgcgggcccg tcgtctcgtc
     3661 ctgctccagc gtgaacaggc cggcgaacgg ggcatcgccg tcgcgttcgt tttcctttcg
     3721 cgcgcgcagc gagagcagaa agtgatcaag catggtcgta cccggaggcg cggcgccggc
     3781 ggcgtcccga gagacccgcg gcggccatcg tgaatatcat ctgatgaata tagccgccgc
     3841 ttcaaccggg agtcaatcga gatcgtgatc tgaataatcc agaaccggga cccgccttca
     3901 aatcgtcccg cgtccctcgt cctcgtctgc gatcttctgc ttttcgtccc tgttgtcctt
     3961 tatgtccttc gcatcctttt cccaaatccg ccggccggct cccaccttac cctgcgaatc
     4021 ctcttgacaa acgttatccg ataatcgttt actcatcatg tcctatccgg cgtctctcag
     4081 tctctggtcc cgcgcgcgga tgtcccctcc ccgcattttc aaaccgaggt ctgactcatg
     4141 cggtttttcc agccattgct catcatcctg gcggcggtcg gcctgctcct cccctccggc
     4201 tccctctccg ccgccacgga tgcggagtac atcgcccagt tcatcaatta ccagagctca
     4261 tttggccaga tcaacgaccc ggtgccgaca cttgacgaat atttcgcgga gaccattccg
     4321 cgcttcgacg aacaatacgt cgacaatatc caggtctggg tcgaccagat cgccgatcag
     4381 cagaacatcc tcggcgagat gggctggacc tcgtcggccg tctccacctc gctcaacgat
     4441 atgtacgccg tcaccggcga cctcaaatac ctccgcgcca gcctggaaat caccagcgtc
     4501 tcgctccagg tgcgcgacga caagatcggc cgcacgacct tctggggcga gtccgccccc
     4561 ggctggagca ccccgcgcta cgccctgcgc tgggtcattc accccgtcca caccgccatg
     4621 atcacctggg ggatgctcga gtcgctctgg ctggcgcagg ccaaccccgt cgccatggcc
     4681 gagctgggcg acgaattcga ctccatcctg gcccaggcgg ccgaaagcat cgactggcac
     4741 aaccgccagt gggtcgaggg ccccgcctcc gacgagggct attacatcta caaggacaat
     4801 gaaccggacc gcgaggggca gcggcttccc ttcaatcgcc agagcacgat gggacgctct
     4861 ttatggatgc tctacaagct gggcggcaac cccgagcatc gcgacaaggc gatcaagctg
     4921 gccacgttcc tcaagcgccg gctccacctg tttctcaccc ccgacaccaa cgagggggtc
     4981 tggttctggg aatacacgct ccccaactcg ccgcgcaaca acccctatcc ctggtcgcaa
     5041 ctctggcgcg tcggaacggg cgacgacctc tctcacggct cgctctccat ggagttctac
     5101 gtcctgatgg ccaacgacgg ccaggtcttc acccacgagg acatgcaggc cctcgcgcgc
     5161 accgtcacct acggcttcgc ccgcctggat aacggcatcc tcttcggcaa cgtcgccggc
     5221 tcgcccgacc tgcatgatcc gtcctacgtg gccaacaccg gctactggct gcggctgacc
     5281 ccctacaatc cggaagttta cgaccggatt gcggccttca tgctgaagta tcagcaggaa
     5341 ccgcgcgggc tggatctggc caaactggtc cggtggaaaa tgccctcggc tgtcccccaa
     5401 tcctggctct acgaataggt aatcgtccct cgtcccccgt tctcgtcctc gtatcgctcc
     5461 cgaaggccat tttcaaaatt cggctctcgg ctctcgnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnctcg gctctcgaaa gccgccctct cgcccacgcc ctcggggatg
     5641 gccagccgcc gcctgggacc gccggcatcc ttgccggcac atggcgccgt catgacaatc
     5701 catcaccgtc ccaaatccaa cccatctccc atgaccactc ctgagccaac gccgtcaatt
     5761 ggggtccgcg tgaactactc cagcggggcg ggatgcgaac agccccaggt ggagcgcagc
     5821 ggaatctggg gacgccgacg cggaaaatcc ccgtccccgt cgggggctca tccgcaattc
     5881 gtccccatta cttccccccg tcttccccgg gctcacgcgc aacccgcgac gtaaacgccc
     5941 gcccgatcag ctcgttaacc acgaaaatcg gaatcaacgc cagcccgaca acgccggcca
     6001 gcacagccag cggccagcgc agccaggtgt cgcccgccgt cgcggccagc ccccagcgca
     6061 cccagcattt gcccagcagc gtctcgagcc ggccgcgggt ctccgccaac tctccctcca
     6121 gccgccgcag atggcggtgg cgttcctcga acgcgagccg ggcggcctgc ttgtcggcgc
     6181 ggacggcggg ggcgcggcag aaccgcgcga gcggctcaat cgtccgctcc cagttcagtt
     6241 cgccacgcac cacctcgagc gccgcctccg cccgggtcgc gatcggctcc tcgccgctca
     6301 gcacccggcg cagcaacgcg cccaggcctt cggcgtcgtc cggatccagc gcccagccgg
     6361 cccccgcccg cgcaatcagc gcgcccagct ccgagtagtg attgtgaatc accggcaggc
     6421 cggcggccag gtagaccacc gtgcgggtcg tgaaggcgat ttcgcgttcc ggattgcgcg
     6481 ccagcaggtc gagcgcgacg cccccctcgg ccagcagccg cgcatatgcc tcgaacccca
     6541 tcgccgggac gaggcgcacc cgcgggtgtc ggcccagccg gtccagcagc gcctcaaact
     6601 gtccccgcga cacatcgaag gtcggatgcg cgccgccgac gaacagcagc tccccccgcc
     6661 ccgtggcgtc cagcgcgtcg agcgccgtct ccagcgcccg cgtcggatcc tgccagggca
     6721 gaaagaatcc tccataaatc aaccggtcgg ggcgcacgcg cggccggtcc atcagcggct
     6781 cgaaactgaa cggaatcacc ggcaggacgt cggtcgcggc caccggccac ccggccagcg
     6841 cgaggtaggg gaggaaatac aaccgctggc gttcgcccga tgccgtcaga aaatccgccc
     6901 ggcgcagcgc cgccagtttc tcctccagat cggcctcgtg ggtcgtgctc ccccagtacg
     6961 tccgttccag caggtgcgga ccggccagat cgatcgccag cggccgatcc ggttcgccca
     7021 ggtcgcgcgc cagcccccag tgctgcagga cgatcacgtc ggggtcatgc tcggccagcg
     7081 ccgccgcgat gttcgcgcgc tcgaacgtgc cgacccactc gggccaaccc cccgaaccct
     7141 tcggttcggc ggcggcgggg gcgaacgtgg ccgccgcccg cgccggcatc agcagctgca
     7201 cgtccagccc cgcctgccgc agcccgcgcg ccagccccca cgcgcgcaga cccgcgcccg
     7261 tggccggcag cccccccacg ggcaagggct cggtggtcag tagcagcacg cgcgacaaac
     7321 gggaaccctc cgctccagct cgttcctcaa catactcaag cgccggaacg aatggcaggg
     7381 aaaagggcgg cgatcggccg actcgcgctg ccgattccgg ctcgcagcga aaccgcgatt
     7441 tgctcccgac ccgccgatgt gtagaatggt gaccaaccag tctgccgggg gaagcgggaa
     7501 gggatagctc catgaatcag ccgaacgatc ccatctggac gacgatcgtc gaggaggtgg
     7561 agcaggccgc gcgcagcgaa gtgatgctgg cctcgtttct ccacgccacc atcctcaatc
     7621 acgaacgctt cgaggacgcg ttgagtttcc acctggccag caagctgggc tcggcgacga
     7681 tccacaccat gaccctgcgc gaggtgatct atcaggcgct gaacgccgat ccggccatcg
     7741 gcgaagcggc ccgcgccgat atccatgccg tccgcgaccg cgatcccgcc tgccgctatt
     7801 actccacgcc gctgctctac ttcaagggct acagcgccct gcaggcgcac cgcatcgccc
     7861 attggctctg gcatcgcggc cgcatccaac tggcgctcta tctccagagc cgcgtctcgg
     7921 aaatcttcgc cgtcgatatc caccccgccg cgcgcatcgg caagggcatc atgctcgacc
     7981 atgccaccgg catcgtcatc ggcgagaccg ccgcgatcga cgacaacgtc tcgatgctcc
     8041 atggcgtcac gctgggcggc accggcaagg aacgcggcga ccgccacccc aagatccgcc
     8101 aggggtgcct gatcggcgtc ggcgccaagg tactgggcaa tatcgtcgtc ggcgagggtt
     8161 cgatcgtcgg cgccggcagc gtcgtcctgc accccgtccc cccgcacagc cgcgtcgtcg
     8221 gcgtgccggc gcgcgtcatc ggccgcacgc acggcgagga tcccgccctc gtcatggatc
     8281 aattcgtcga ccgcgacccg aacgacccgg aaaacggcgc gcaatgaagc tcgtcacggc
     8341 ccggcagatg cgcgaaatcg atcgcatcac gatcgcggag cgcggcgtcc ccgccgccga
     8401 gctgatggaa cgcgcgggcc aggccgtcgc tcgcgaggcg ctcgaacgct tgcaccccga
     8461 ccgcgtcgcg atcgtcacgg gcaagggcaa caacgccggc gacggcttcg tcgccgcgcg
     8521 cgccctgcac caggcgaacg tccgaacgac cctctacctg ttgcgccccc ccgatgaatt
     8581 gcagggcgag gcgctcgccg cctggcgcgc cgtccccgcg ccgatcgagc ggatcgtcgt
     8641 ccagtcaccc gccgatctcg cccgcctggg cgagcatgat ctgatcatcg atgccatctt
     8701 cggcaccggc atccgcgggc ccgtccagga accctgggcc agctacatcg cggcggtcaa
     8761 cgccgccggc gcgaacattc tttccatcga catcccctcg ggcttgcccg gcgatccggc
     8821 cgaggaaccg gggccgcacg tccgcagcca gttgaccgtc accatcggcc tgcccaaggc
     8881 ggggatgctg ctcggcgccg gcctccgcgc cactggccag gtcgtcgtgg ccgacatcgg
     8941 cttcccgcgc gagctgctcg aagaccccgc gatcacgctc aacctgatga cggcggagga
     9001 agcccgcgct ctcctgccgc cgcgcgatcc cgccggccac aaggggacct tcggccgcgc
     9061 gctgatcctg ggcggcagcg agggcatgac cggcgcggcc gtgctcgcgg cgcgcgccgc
     9121 ggcccgctcg ggcgccggcc tgatttacag cgcctacccc gcgccgctcg ggccgatcat
     9181 ggaaagccac ctcatcgaac cggtcaagct cccgctcgaa ggcgacgagc ggtggttcac
     9241 cccgcgccac ctcgaagccg tcctggagca cgccgagcgg atggacgccg tcgcgctggg
     9301 cccgggcatc ggcacgcgca aaccgaccgc gcgcttcgtc caggccgtgc tggaaaacgt
     9361 caaggccccg atggtcgtcg acgccgacgg gctgaacctg atcgcgaagc gccgcgtcga
     9421 cctccgccgg cggccgggac cgaccgtgtt gacgccgcac cccgccgaag cggcgcgcct
     9481 gctcgagctg gaaaccggcg aggtcgtccg ccgccgcctc gacgtgatga ccgagttcgc
     9541 ggcgcgccac aacgtcgtcg tcgtgctcaa gggcgcgcag acgctgacga tcgctcccga
     9601 cggccagcgc accgtcaacc cgacgggcaa cagcggcctg gccaagggcg gcagcggcga
     9661 tgtcctgacc ggcctgatcg ccgggctgct ggcccagggc ctgagcgcgc tcgacgcggc
     9721 gcggctcggc gtcttcctcc acggcctgag cgccgacgtc gtcgcggagc gcatcagcgt
     9781 gcgcgccatg ctccccagcg atctgctgga cgccttcgga accgcgttcc aacggctgga
     9841 acgcaacgcg ccctgacagc ccgcgccagc gggctttttc ctggccccgt cggggacctg
     9901 acattggcgc cgggcctcgg cccggtgacc gcacgcgaaa gaaacttcaa accgtttcaa
     9961 acggtttccc cgccacccag ccgagccgat gtcagccagc ccgcgccacc accccccgtt
    10021 ccaggattgc tgttcttgaa attccagaat ttccatctgt ttcgattcga atttattaat
    10081 tcgaatttca aacttccttc ttctcccgcg cctttcgtca tccgcttgct ctctcactcg
    10141 tagggctccc acacctccaa ctcaacgccc gtctggatct tcgagagcgt cacatcgacg
    10201 agcatcgggt tgcggccgac cacgtcgttg acgaccagca cgcgatcgct gaaccggccg
    10261 tcgcccagcc ccgtcgggtc ggcaatcacc gtcaccggca gctcctgccc cggcgccagc
    10321 ttgcgcggca ggctgtcggc cgtcacaacc ccgagccacg tgctgccgct ctgcaccacc
    10381 acgccataga tcgtgaacga ggcattgccg atgttgcgca ccgtgaacgt ctgcggcgcc
    10441 agtaccgtcg gcgcgagccg gccggtcgcg gtcgcgctca catgccctcc cgtctgcgca
    10501 ttgcgtgcct tcacgtaata gaaatacgtg atgccgggca gcgctgtgtg gtcatcgaac
    10561 atcccctcgg cgatccacgg gctgatcggc agcgcggccg acgggttgtc gacccccgtc
    10621 gtccggtaaa cctggtagtc ggtcgcctgc ggcacgttcg cgttccaggt gacgcgcacg
    10681 tgatccagca ggttgctcga ggccgcgacg ccgccgggcc ctgccagcag cgcgaccgaa
    10741 ccgacccggc ccagaacccc ctcgctccag accggttcgc tcccaagccc gcggacgaag
    10801 taatagtaat tcacgcccgc gcggacgttc cggtcggtcg cgaacgtttc cgtcatgctc
    10861 ggggtgatgc tcttggcctg cgcgaggtcg ttgaccccct cgctgcgact gacgaagtaa
    10921 ctgcttgccc cgacatcgtt ccacgtgatc tcgacgcggt cggtcagatg gatcgtggcc
    10981 aaggcgacgg ggctttgtat cggggccttg cgccggccca gcacgctgtt gctgaacgaa
    11041 ctgatctcgg tcgaattgac ccccagaaca taataaaagt aatccacgcc cggcgccgcg
    11101 gtcgtgtcgt cgaacgtcgt cccgctgagc gggccggcga tgatgctggc ccccgccgtc
    11161 gtgttggatg tattgcgata gacgtaatag gccagcgccc cggcgagtgg attccacgac
    11221 agcgtcacgc gatccgtcag gttggtcgaa gcggtcaggc cggtcggcgt ccccagcgcg
    11281 gccgtcgtct tgttgagcac gacatcgtcg atccacaccc cgtagcgggc cagattgtag
    11341 ttggcatcgg agacaaactg gaactgcacg taacacagct cgtttccaag gatattgccc
    11401 agtcccgaaa cattgctcag atcgacggtg aagttctgat tcgccgccag acccgacgcg
    11461 acgtagtacc cgtactccgt ctgattgcgg tagaccagga cgtaaaacag atcgccggtc
    11521 gtgccgttcc acgcgtcggt atcgaggtca taccagaact ggaacgaagc cgcggtggcg
    11581 tcggccaggc tgaagggacc gaacgtcagc gaggtcatga agttggccgg ataggaatcg
    11641 atctgggggt cgcggcccag gctgccgccg gcggcgcacc acagcgcgcg caacccgctg
    11701 ttcttgcgca gatcgtcgaa ccaccagtgc gggtcgtccg tggccgggat ataccacccg
    11761 gccgccagcg tgtcgcccac ttcaaacgag tcgttgagga tcccgagttg ccccttcggc
    11821 agttggatcg gcttggcgtc gcgcaacgcc tccggaatca ccggcctggg agggggcgcg
    11881 tccagcgccg catcgcccgg ctgcaggccg gtcgtcccgg cggtcttgcg ccgggtgtcc
    11941 agcgagaaat tcacgcttgc gggctgcacc gcgatgtcgg caacgcccgc gccgtcgagc
    12001 ccggtttccc acatcccccg gccgaacgtc ccggcgcgca gcagccgctg cggtgcgaaa
    12061 tacgccaggt cggtcacgac cgtcatcggc agcccggcgt tgaactcata ccacgtgacg
    12121 ccatgatcgt ccgtccggta gcaccccaga tcggtgccga cgatcaccgt gctcggattc
    12181 agcgggtcga tgaagatcgc cgagaccggc agttgtcgcg gcaggttgcc ggtcttgtct
    12241 gtccatgtct ggccgcgatc ggtcgaaagg tagatccgcc cgttgggcgc gtccttgccc
    12301 tggccgtaat ccgtatagca gagccagacc cagtcattct gcgcggggtc gcacttgatc
    12361 cggccgagga ttccctggcg cggcgggtcc agctcgcgcg gttcgctgat cgcgccgctg
    12421 ccgtcgccga tgtccatccg cagcaggccc ggcacgtagg cgcccagcgt ctgatcgaaa
    12481 cggttggtcg ccacgtagat ctgccgattg cgcaatccgc tcgatagatc ggcgtgttcg
    12541 aacgtatggc tgaactgctg gtccatttcg agcagttttt cccattgcgg gacggcgccg
    12601 ggcgtgaact gcgtgcggta tatatggttc agcacgacgt acgccgtccg cggttgctgc
    12661 ggatcgagca ggaacggcgt gacccacgcc ccctgttcat tttgcgggat tccgttgggc
    12721 atgctccccg cgtcgggaaa tccattgtag gtccgcaggt aatcgtgcca gctctggctg
    12781 ccgttgccgg tgtacttcat caggttgccg aaataattca ccccgtagaa cccgtcgcct
    12841 tccgggtcga gcggatcaac cgccacctcc atgcagtcgc cgaactcatt cggccagtgg
    12901 cgccacgtcc cgtcgggccg gcgcacatac ttgtcgttgt cctgcgtccc catgatcacc
    12961 gcgttggcca tcgtcggatg gttgtcgatc cggtagatct gcgtgcagtt gagccccgcg
    13021 ttgcgttcga tccaggagtt gcccccgtcg gtgctcatga agcacccgcc gtcggtgccg
    13081 atgtagatcg tattggcgct cgccccgaag gcgatcgcgt ggtggtcggc atggatggag
    13141 ggcagccccg cgttgttctc cggatcccaa tcggtcatcg cggcccagct ctgcccgccg
    13201 tcggtgctgc ggtagctgcg caggttcgtg cccagataga tccggttggg atcggccgga
    13261 ctgaccgcga tgcagttgtt ataattcatc tgttccgccg cgctggtgcc tgccttgggc
    13321 aattccgtcc agtcctgccc gccgtcggcc gaggtccaga tccccttgat cgaactgttg
    13381 gtgctggcca ccagggcgta gaggcggttg ggattggacg gatcgcgggc aagctcggcg
    13441 cggcgtcccc cggagcgatc gggcgtcgtg accgccggat tgaacgtcag acccccgtcg
    13501 gccgatgtgt aaagcacgac ttccgccgta gcccccgccc cgcgccaggt cgagaccgcg
    13561 atccgcgaac ggttcgtcgg atcaatcgaa atcgacaccg ccacctcgtc gatccgcttg
    13621 acccacgact gccccccgtc ggtcgagcgg aacagaccgc ggccggcctg gtcgtgcccg
    13681 ccccacgccg ccgccaggat cgtgtcggag ttgaccagtt tgatgtcgcc gatgaacgcc
    13741 atcaccccgt tgcccgtccc cggcagcagc gcccagttct ggcccccgtc cgtcgacttg
    13801 tagatccccg tcccgagcgg gtccatgccg ttctggtgac ctttgttgcc ctcgcccagg
    13861 ccgatgtaca gcgtgctggg ctggaccgga tccatcgcga tgctgccgac gcccagcgtc
    13921 ggcaccttgc cgtcggtcag gttgtaccag ttcaacccct ggtccgtggt cttccacaac
    13981 ccgccgccgg ccgtcgcggc atagaggatc tgttcgttca gcggatcgac caccagatcg
    14041 gtgatccggc cgttgggcgg cgcatccacc tggccgccgg ggcccagata cgtccacgcg
    14101 gcgccgacgt ccttcggtct ccgctggttg tatttccggg agtgatccag cgcctcctcc
    14161 cggtaggcga tcggcaccac ccccgtctgg tcgacccggt ggttgtaaaa ccacagccag
    14221 gccagatccg gcttgccctg gcgcttgtaa tcggcgctct ggcggcgcgt ctgccccggt
    14281 tcgagcgcaa agctcggcat cagcggacga taacgcgccg cggagtcgat cgcggccgct
    14341 tcgcgccgcc gcggcaggga ctggcagccc agcacgctta ccagcacggt gagcgtcagc
    14401 acgacgagag agatggattt catggacgga gagtgatcct cgggcgggcg gagcatggca
    14461 ccccggcccg ccgcggcggg gagaatgggt gattgattga tgatccctga agcggccgcg
    14521 ccagcgcatt cgcggccgcc gggagggatt gtaacggaac tctccgagcg gacgaagcga
    14581 aaaaatgatc cccgcgtatc ttttacgcct cataacgccc agggcgccct caggatctca
    14641 ctcatacgct ttccacaacc gcagttccac gcggttctgc tcgatcgtca gcgtgatctc
    14701 gacccccgtc ggatacggat ccttgcctgg cacgttgttg tcgatcacca gccggtcgcc
    14761 atgctcgccc tggcccagtc cggtcggatt ggcgacgacc gtgaacgtct gcgcctggtt
    14821 cgggctgatc ttgatcggat aggccgaggg aggcgtgacc ttcagccacg ggctgttgtt
    14881 ctggaacgtg atccccgtga tctccagcgt cgcatcgccg atgttcttga tcacgatatc
    14941 ctgcggcgcg atcggcggcg gcgggatccg ccccagcgcc cccgcgctca ggttcccggt
    15001 gctctgggcg ttgcgtccgc gcacgtagta gtagtaatcg acgccgggca cggccaccag
    15061 atcgtcgtag ctggtcgccc cgatccagcc gctcagatcg ttcgcctggc cggcgttatc
    15121 gaccccctgg ttgcgcagca cctggtattc cgtcgcctgc gccacggggt tccactggac
    15181 ggtcacgcgg tcggcgagct gcgtcgtggc gatcacgccg gtcggcggat cgagcggcgg
    15241 cgccgcggcg cgccggccca gcaccccgct gctcagcggg ctggtctgcc cgccgttgcg
    15301 cgcctgcaca aagtagtagt aatcggtctc cgccaaggcc gtcaggtcgt cgtagctggt
    15361 cgccccggcc caattgccca gcggcgtggc ctgggccgcg ttgttcgtgg tattgcgctg
    15421 gacccgatac tccgtcgctc cggctaccgt gttccagttg atggttaccc tgtcggtcag
    15481 gttggtcgtg gcggtcaccc cgccgggcgc ctgcagcgga tcgttcacgg tcttggtaat
    15541 gacgacgtcg tcgatccaca cgccgcgctt gctgttgttt cggaagaagt ccgaaaggaa
    15601 cacgaattgg atcatgacgt cgtcctggcc cgtcaggtca ccgaggaatt aatccgtctt
    15661 caggcttttc tcgaacgtgc cggccagcgt gccggtgaag tagtaataac cggcgctccc
    15721 cgggtccgcc tggcggtagg cgtaaacccg aaattcctcc ccgagcacgg cgaaatcgat
    15781 atccacgtca taccagaatt ggagcgtcgc ctccgtcgca tcggccagac tgaacggccc
    15841 gaacgtcaga tacgtgatca cgccctcggg gtaggtgctg aactccggat cgacgccgaa
    15901 aaagcccccg cgggcgcacc agaatgaaaa cgtgcccgaa ttgctcctga agtcgtcgaa
    15961 ccaccagtag ggatcttcaa atggattgat gaaccaccca ttggccagcg cgcccgtatc
    16021 aaaggtttca ttgaggattt gcatttccgc cttgggctgg tcgatcggcg ccgccttgcg
    16081 cagcgcctcg gtcatcaccg gacctcggcc gtccttggcc gccttcccgc agcccatgcg
    16141 ccccgccgcc tgtttgccgt cgctgaaggt gaaggccagg ctctcgggcg tcacctcgat
    16201 gtcgggcgag ccccccgcca gcggcgtctc ccacagcccg cggccgaacg tccccacccg
    16261 cagcttgcgc tcgggcggaa aatagtccag atccgtcacg accgcattgg gcagcccgtc
    16321 attgtactca taccacgtcg cgcccccgtc gtccgaccgg tagcacccca gatcggtccc
    16381 caccaccagc gtggccggcg cctccgggtc gacgaagatc gccgagaccg gcagatggct
    16441 cggcaggttg cccgtccggt tttcccagct ctgccccttg ttcggcgaca tgtagacccg
    16501 cccggcggga ttggccttgt tctgcccgta gtcggtatac gtgacccaga tcgaatcgtt
    16561 ggcgaccgga tcgcacttca gcgtccccag gatccccgga cgcggcggcg tcggaaacac
    16621 cgcattgctc gccgccccca gcccgtcgcc gatatccatc cgcagaatcc ccgggtcgaa
    16681 cacctgctgc acctgatcga aacgattcaa cgcggcgtag agctggcggt tgttcgtccc
    16741 cgacgacagg tccgcgatct cgaacgtccc acccggcacg tagttggcat tgatcgtggc
    16801 cagttgctgc cactgcgcca ccgcgcccgg cgtgaactgc tgtcggtaga cgttctgcag
    16861 aatgacccac acgctgcgcg gatgctgcgg atccaccagc agcggcgcca cccacgcccc
    16921 ctgctcgtta tccgggatgc cgttgggcgt cgaattgatc tcgggatagc ccgtgtaggt
    16981 gcgcaagaag tcgttccagc tctggctgcc gttgcccgtg tagcgcatcg tcgtgccgaa
    17041 atagttcatc ccgtagaacc cgtcgccctg ggcgtccagc gtatcgacgg ccacttccat
    17101 gcagtcgccg aacgaattgg gccagtggcg ccacgtcccg tcgggccggc gcacatactt
    17161 gtcgttgtcc tgcgtcccca tgatcagcgc gttcgcgatc gtcgggtggt tcgcgatccg
    17221 atagatctgc gtgcagtcca gcccctcgtt gcgctcgatc caggtggcgc ccccgtcgct
    17281 gctcatgaaa aacccgccgt cggttcccat gtagatggtg ttgcccgtgc tgccgaaggc
    17341 gatgacgtga tgatcggcgt gaatgtaggg gatcccgtcg ccgtcgtcgg cgttccagtc
    17401 ggtcatcggc gtccagttca gcccgccgtt ctggctccag tagctgcgca ggttcgagcc
    17461 gaaatacagg cggttggaat cattcgggct gaccgcgatg cagttgttgt agttcatctg
    17521 gcccgggccg ctgtcgccgg cggggatccc gttcgtcgtc agcgcgctcc agttctggcc
    17581 gccgttggtc gagctccaga tccccttgat cttgctgtcg ccggtcgcca ccagcgcata
    17641 gagcttcgtt ggcgtcgtcg gatcgcgcgc cagttccgcc cggaagcagc ccgtcgcgtt
    17701 gggatacgtc gccggattga aactcgcccc gccgttggtc gagtagtaga accgggtggt
    17761 cgtccccacc cccgtctcca ccacccatgt ggtcaaggcg acgttgttgc tgttggtcgg
    17821 atccactgag atcgagactg ccggctcatc cacgatgcgc gtccagtttt gaccgccgtc
    17881 ggtcgagcgc cacagccccc gccccgcaag ctgatacccg ccccaggccg ccgccaggat
    17941 cgtgttggcg tcgacgaacc ggatgtcgcc gacgaattgc atcgtcccgt tgcccgtccc
    18001 cggcaccagg ttccacgtct gcccgccgtc ggtggtcctg taaatcccgg tgccgagcgg
    18061 gtccacgtcg ttgccgaacc ccttgttgcc ctcgcccagg cccagcagca gcgtctcggg
    18121 gttgcccggc tgcatcacaa ggctgccgca gccgagcgac gggattttcc cgtcagtcag
    18181 gtttgtccag ctttgtccct ggttgatcgt cttccagacg ccgccgccgg ccgtcgccgc
    18241 atagatgatc tgttcgttct ggggatgaac cgccaggtcg atgatccggc cgt
//
LOCUS       DAOPCO010000048        18044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3209_length_18044_cov_5.947579, whole genome shotgun sequence.
ACCESSION   DAOPCO010000048 DAOPCO010000000
VERSION     DAOPCO010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 18044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18044
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3209_length_18044_cov_5.947579"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLS90_05550"
     CDS             <1..582
                     /locus_tag="PLS90_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01902.1"
                     /translation="LRHIAQNYGVRVPVVSSTALYVIPPDQWGNFVDGLEALWEKLRG
                     PVVREPDRLNLLPHCEEFNNTHGFHEVELEDNGAFRWTRGPFQRIQFRAPERPGRYRL
                     SLHFYRPAPYPSATMPIDYCFTGDGMFKQMTAGVGSVTVEDVVETTTSRQPMVLELFT
                     DYFVPAIFQENTNDRRELGMMFLRLTMTPADVP"
     gene            1081..3099
                     /gene="thrS"
                     /locus_tag="PLS90_05555"
     CDS             1081..3099
                     /gene="thrS"
                     /locus_tag="PLS90_05555"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPK01903.1"
                     /translation="MNITLTLPDGSQKHFEAGVTGRQVAESIGAGLARAALGIKVDGE
                     LRDLDRPIAADAQVAIVTAGRDAPDADALYLLRHSCAHVMAEAIQRLYPGTKLAYGPP
                     VENGFYYDIDCPSAITEEDFPRIEAEMERIIGEDRPFTRYEMSRAEAERKLAGDEYKL
                     DNVARADGDVMSFYATGEPGRDWEDLCRGPHVPSTGRIGAFKLLSVAGAYWRGDASRQ
                     QLQRVYGTAFPDKKQLKAHLAALEEARKRDHRVLGRQLGLFHIDEAVGQGLVLWKPKG
                     AVVRQELQNFISEELRRQGYHQVFTPHIGKLGLYRTSGHFPYYRESQYPPIIEHDELA
                     RLADEGATCAELSNRMAAGTVDGYLLKPMNCPHHIRIFASEHRSYRDLPLRLAEFGTV
                     YRWEQSGEIGGMTRVRGFTQDDAHIFCTEDQVTAELLGCLSLVRTVFQTLGMHDYRVR
                     VGLRDPDSGKYVGAPENWDKAEAACRAAARSLGVAFSEEPGEAAFYGPKIDFVVKDVI
                     GREWQLGTVQVDYNLPERFALEYIGADNSAHRPVMIHRAPFGSMERFVGVLIEHFAGA
                     FPPWLAPVQAAVLAITDAQLDYAREVLEQLKEAGLRAEIDASSEKIGKKIRAAELAKI
                     PFMFVLGAKEAESRSVALRRHGKGDEGVLGLAEAVALLKAKVTERALA"
     gene            3151..4473
                     /locus_tag="PLS90_05560"
     CDS             3151..4473
                     /locus_tag="PLS90_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HPK01904.1"
                     /translation="MRYDIAIIGAGAAGLAAAIFAGAAAAAGSAARIVLLDGAKKPGA
                     KILVSGGGRCNVTHREVRPEDFNGGPQPLIRGVLRAFDHRDTLRWMAGLGVALKLEST
                     GKYFPVSDKARTVLEALLRRVGELGVELRAGEKVVEIDRRDSHFEFRHSLEGETGGDS
                     WFDLQLAGGGVLGVRRVIVATGGMALPKSGSDGAGLEWMRRLGHGIIPAVPALCPLVL
                     ADEQPSAAAARVRELAGVTVDARLALVEENSRRLGETTDSLLFTHFGLSGPAAMNLSR
                     HWARARDAAPATPPVLALGHPSLPTEERADAWLREQAAAHPRLAVGACLRRLWPERLA
                     LLLAGEAADTALNGLTRMRRRALAGALAGLRLRVAGPRGWAFAETTAGGVDLREVDIR
                     TMQSRKAPGLYLCGEILDVDGRIGGFNFQWAWSTGYLAGRGAAASLRG"
     gene            5225..6379
                     /gene="pilM"
                     /locus_tag="PLS90_05565"
     CDS             5225..6379
                     /gene="pilM"
                     /locus_tag="PLS90_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPK01905.1"
                     /translation="MFKSKTPIGVDIGSHSVKVCQLRRTGDSYELERFGMADIYPEGE
                     PPRDAAGQTEAKTEALKRALASAGIKSRQAISSVCGESIIVRYLQLPEMPENELKKAL
                     QWEAEEYIPFRLSEVNLDSAILGRVPDGEIPKMDVLLVSAKKDLVQRHVSVLRGAGLE
                     PRVVDVDSFAFMNCYELNYEQGDECTALVNIGSEITSINVFSAGTSRFSRDILIGGQT
                     ITQAIRSHLGCNFADAENLKMAYGTVPATGGDEDGGEGASGLLDTIRGTIEEMTEQET
                     SRDAQEYAVRKAVQAVVHNLLVEVRRSIEFFEGQYRGINVGRVILGGGTAGLPNLREN
                     VEQELRLPVELMDPLRRVRVPQRSGFMSQAEQMRHQLGVAIGLGIRGLAA"
     gene            6432..7268
                     /locus_tag="PLS90_05570"
     CDS             6432..7268
                     /locus_tag="PLS90_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01906.1"
                     /translation="MIQINLLENSSTRHRARTAAAGGGAAGGAGTGAGAGGLGAIVWL
                     ALILVLGANGGLGWIAWSKVKVAGDEFRAAERKQKQIEEQIEKQLTEAEQVRKFREVI
                     NNQLDVLRSLDPPDRILWSQKVNMLANLIPANVFLTELDIQEDVTMVETEASKAAREA
                     WKKSKAEDKGKEPQPVMRPVIKYIVRLTGVALGNDAHQQFENVTRFHHAMINHVEEDA
                     QGGTVRFMDGFQSNIEFEKVEATVYQGTPCNAFTFKLTTHPMGQQENAPAAEKRQVAS
                     AR"
     gene            7336..7884
                     /gene="pilO"
                     /locus_tag="PLS90_05575"
     CDS             7336..7884
                     /gene="pilO"
                     /locus_tag="PLS90_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPK01907.1"
                     /translation="MSDQQKTALLVTVFVGLVGLILIAYFHFMIGRGMINKYERRTRD
                     MVEETKELEVQLAEIRSLLSQKDELEKQAEMIEKVTRRLPDSPDAPGFLNALVMILGT
                     TGIIQEEVKPDKNTARALYTEIPYSVKAHGHYHAFGQFLTLVEQNPDRFMRLKKLTIG
                     NNAERPSVHPIEMEITTFMFNN"
     gene            8022..8948
                     /locus_tag="PLS90_05580"
     CDS             8022..8948
                     /locus_tag="PLS90_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01908.1"
                     /translation="MRTNQPVRADLVRGGIRWGLLTAALLVALPATAESPWRRLARTL
                     STEPQTTLPAVPQDERLRGVEGMTTGMPTLPEVPDEGTLIPAVYGDAATTGVAAPAGG
                     AGALAALQGPKSESGKISLGLESEDAPVMATDLFQQPEIRQMLGDEPRFVYNPNGRPD
                     PMLLPWVRNEAIFKELSAQAEALEEAGEYDAAITLYQRIQRMGDPRFNLIAQDKIMAI
                     AQAQAAQVQSAMAEAYVPEIKVELPNWVAENTTGVIVGEDRSLCLVGDFMLAEGEALP
                     NYPEVRVAAISDKLVTYQVQHETFEVELKHEQ"
     gene            9044..10912
                     /locus_tag="PLS90_05585"
     CDS             9044..10912
                     /locus_tag="PLS90_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPK01909.1"
                     /translation="MAPFPQQIRLMAMALAIAATAPWLGAQGSGDPGGTADALNQYVA
                     HAENEVRLAAAAPVDVVIPDPNGDAEPRPAMPGEVAQNVSGTGDEAMQVNPDETIALD
                     FEDSEQDILTRQALEKLDVIVESLEFANADLRNVIRIIGERLDINFIFDADDIGGKVS
                     LRLHNVRLRDALESILSTRKLAIVPDQSGIFRIVPQEQIGRQLVETRTEVIQLNWLSA
                     DDVLETMEPFLTDHGRMMANEESNTLIITDVPPQIQVIRDLIAEIDLAERQVLIEARL
                     ADINVGALRNLGTQWSVTKLNEHALRRLDSRSMDKDSTGLFGPAGVNNVETVTRDMYL
                     GGNLIGSTEVTGLAGAVEALDPTPLANAGGLIPVLLEGFTLGGGLGSLSFGDKIGIFG
                     DEYDLNATFSFLEQRDIVEVLANPRVTTLNNVPARIDIIEKIPYIESVQGPTEGVVIG
                     EIEFEPVGVTINVKPIITPNGFVRMEIDLRQKIFRGRVDAGGGGFSAYDPPQVDERNA
                     MTNVIVRNADTVALGGFRQVRKVEGVDGVPWLHRIPVIGWLFKNKEHDQAKTELVLML
                     TPKIVEQHVGLSDREKYLYEKIDHDWHVPDKFFDETLGADEKDQQARRAARFGKVN"
     gene            11279..12898
                     /locus_tag="PLS90_05590"
     CDS             11279..12898
                     /locus_tag="PLS90_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK01910.1"
                     /translation="MSEISRGGERAGAGGPGSSAPIEPPAGSPVAPVGAAAPSPKIRA
                     ELETLVRARYPLLQIVSWEEERVMECLDGIATALGKHLWQWSINSGLCTYRRFGGMQQ
                     EGQKGTRDPLVALREISTAAAEPGIFVLKDFHPFLGEASVVRALRDLAAQLRTSYATV
                     ILLGPTLRVPAELEKDLTVVDFPLPDRDDLAAFFDQLAKDLQGNPELHFDNTDETRQR
                     LLEAAIGLTMNEVENVFAKILVRRGRLTVQEVPDVYQEKRQIIRKSGVLQYIEPSESI
                     EMVGGLAGLKEWLRKRRRAFHPKARQFGVPVPKGVLFLGVQGCGKSLAAKAVSRYWQM
                     PLLRLDMGELFGSLVGKSEENARRAVTIAESIAPVILWIDEIDKGFSGLASSSFSDSG
                     TTARVFGTLLTWLQEKTAPVFVIATANDVKVLPPELLRQGRFDEIFFVDLPSPAERRE
                     IFGIHLKLRRRRPDRFDLEELARRAEGFSGAEIEQAVISALFDAFDEGRDVGQEALLR
                     AIAATRPLSVLMSDEIAERRNWARGRTRPAS"
     gene            complement(12907..14367)
                     /gene="xseA"
                     /locus_tag="PLS90_05595"
     CDS             complement(12907..14367)
                     /gene="xseA"
                     /locus_tag="PLS90_05595"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPK01911.1"
                     /translation="MSTAGNPPTLNGLGDRPLTVSELSWRVQHLLEEAIGYVRVEGEV
                     SGVRIPSSGHCYFVLKDERTAINAVCFRSTLARQKVRPADGMKLEVRGRVTAYTARSE
                     YQVIVDSLAEAGLGELMRRYLELKDQLAAQGLFDEARKRPIPRLPRRIGIVTSATGAA
                     LRDMLNVLGRRARGITIYLAPAAVQGAQAAPAIVAALQRLQRHGRAEVIIVGRGGGSI
                     EDLWAFNEEPVVRAIAACSIPVISAVGHQTDTTLADFAADLRAPTPSAAAELVTAHYG
                     ELAESLAVLCRRLARAIARDLERRRARLDRCRHAWGLRRPPERLQLMMQRLDDLAADL
                     TARMQRTLRDRRDALAIAGLRLGRCNPRARLELLHTRLDRLRAKLHATGPARWGPELR
                     ANRERIDHLRGRLHAAMRSDLQRARLRLAAAHDQLRAVSPEAVLQRGYSIVTHGKRDR
                     IVKHPDDVKVSEIVRIRSAGGAWRAAALPPGDDLFE"
     gene            complement(14364..15116)
                     /locus_tag="PLS90_05600"
     CDS             complement(14364..15116)
                     /locus_tag="PLS90_05600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01912.1"
                     /translation="MEHEEYQRMFALEDRHWWFQGRLMMIERLLRRHVLPVLPARPRL
                     LDLGCGTGLFLQRRLRECEPFGIDFSVEALACCRTRVPGRLARADAARLPFADAVFDV
                     VSAFDLIEHVADDVALVAEAWRVLRPGGCFIATVPAHPILWSAHDVSLHHQRRYRRAQ
                     FNALFNAAPWETIRMTAGFTLIFPPALLIRLGRRLARSSRPPVSDTRETAEWLNRLLI
                     RLHAGEAAWLERHDLPIGISLMTIRRKPEARP"
     gene            15438..16604
                     /locus_tag="PLS90_05605"
     CDS             15438..16604
                     /locus_tag="PLS90_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HPK01913.1"
                     /translation="MTLRIVADENMPFAAEHFGRLGEVTLLAGRRITADDVRDADLLM
                     VRSVTRVNRELLAGSRVRFVGTATIGTDHVDLDYLRESGIGFASAPGCNAESVAQYVA
                     AALAFAAGRLGLRLSECSLGIVGVGNCGSRVRRVAEALGLRVVLNDPPLARATGDSSY
                     RPLDELMECDFVTLHVPLTRGGDDPTWRLFDGERIARLKPAAVLINASRGGVIDEGAW
                     AAGLSDGPRHKLILDAWEGEPSINPDMLRRALVGTPHIAGYSYDGKVAGTRMIYEAAC
                     RWLGREAAAPALAMPPAAVPAIRLNAAHRAADSILAELVLTAYPILRDDADLRTALHA
                     AGEAIGAAFDERRKRYPLRREFAATTLTLEGADEDLAERVRMLGFTCRNAAASR"
     gene            16895..>18044
                     /locus_tag="PLS90_05610"
     CDS             16895..>18044
                     /locus_tag="PLS90_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01914.1"
                     /translation="MESLRSPVGVRPRALAWLMLGMLLAPRAQPQPTELREKLYCCDP
                     QPELLDLSGTYEGYYPDGVKLFRSVLEHNARRAYGMRDFLTLSLVDRMQTETGELQMY
                     TGQRQISYRPLFGANGVCEGGLFAECVRRPITINYTVSGTVEWLFTPEGAAAKAIHLE
                     GLIAGATETTLDYGTRIRVAYTRGDSANPQNAVVWRDLRGGVLLQIVFGMAQFPEPGF
                     DGGWEWVETFDNPLQDCPCSLSDGTNRPQTMYYQRVSSLPPLQAGADIRMTLEAAQPV
                     EPVGGEFRPEGEIVLLRQPRYVRARGAEESVNDYNAFLANERQAMETTAHRATRDGEI
                     YRFADVPFFQAGGDILQPAYYTIAALNTEIEEAIFDGTSTVTQTTHFIT"
BASE COUNT         3096 a         5282 c         6906 g         2760 t
ORIGIN      
        1 ctgcgtcaca tcgcgcagaa ctacggcgtg cgggtccccg tcgtcagctc cacggcgctc
       61 tacgtcatcc cgcccgatca gtgggggaac tttgtcgatg ggctggaagc gctgtgggag
      121 aagttacgcg ggcccgtcgt gcgcgagccc gaccggctca atctgctgcc gcactgcgag
      181 gaattcaaca acacgcacgg cttccatgag gtcgagctgg aggacaacgg cgctttccgc
      241 tggacgcgcg ggccgtttca gcggatccag ttccgcgcgc cggagcggcc cgggcgttac
      301 cgcctgagcc tgcacttcta ccggccggcg ccctatccca gcgccacgat gccgatcgac
      361 tactgcttca ccggcgacgg gatgttcaaa cagatgacgg ccggagtcgg atcggtgacg
      421 gtcgaggacg tggtcgagac gacgacgtcc cgccagccga tggtgctgga gctctttacg
      481 gactacttcg ttcccgccat tttccaggag aataccaatg accggcgcga gttggggatg
      541 atgttcctgc ggttgacgat gacgccggcc gatgtgccgt gaggggggat ggatcgggat
      601 cgggaagccg gaacgggatc gggtcaacag cgggtggtct gtggttgagc gaaacaccgg
      661 ttattcgaga gccgaggacg agagacgaga gccgggagcc gaagttaggg gaaggccgtt
      721 gagggcgatc acgaggacga gagccgagtt ttgggggcag ggcgttgggg gcgattacga
      781 ggacgaggac gagagacgag tcttggagga tgggggaaat tgacatccgg agggcgtttc
      841 tggcaaggta tgggggagat ttgtgttgga acatccgcca tgagcgcgcc gaccgtcccc
      901 ctccgttcac gcttcgccct cccgacgacg ggacgaacgg ccggtgcgcg gccggccaca
      961 ccctgaacca accccgcgat cccgattttg tccatgcacc gttggcgaga ttcatcgcga
     1021 atcttgcagg cggggccaat ccggtccaga ctaggcgcgg ttgaaaggaa gttccatccg
     1081 atgaacatta ccctgacgct gcccgacggc agtcaaaagc acttcgaagc gggcgtgacc
     1141 ggccggcagg tggccgagtc gatcggtgcg ggcctggcgc gggcggcgct cggcatcaag
     1201 gtggacggcg agctgcgcga cctggaccgg ccgatcgccg ccgacgccca ggtggcgatc
     1261 gtgaccgccg gccgcgacgc gcccgacgcc gacgcgctct atctgctgcg ccactcgtgc
     1321 gcgcacgtga tggccgaggc gattcagcgg ctctaccccg gcacgaagct ggcctacggc
     1381 ccgccggtgg aaaacggttt ctactacgac atcgactgcc cctcggcgat cacggaggag
     1441 gacttccccc ggatcgaggc ggagatggag cggatcatcg gcgaggatcg cccgttcacg
     1501 cgctacgaga tgagccgcgc cgaggcggag cggaagctgg ccggggatga atacaagctg
     1561 gacaacgtgg cgcgcgccga cggcgacgtg atgagcttct atgcaacggg cgagccgggt
     1621 cgcgactggg aggacctgtg ccgcggcccg catgtgccgt cgaccgggcg gatcggggcg
     1681 ttcaagctgc tttcggtggc gggcgcctac tggcgcggcg acgccagccg ccagcagctc
     1741 cagcgggtct acgggacggc gttccccgac aagaagcagc tcaaggcgca tctggcggcg
     1801 ctggaggagg cgcgcaagcg cgatcaccgc gtgctggggc gtcagctcgg gctgttccac
     1861 attgacgaag cggtgggcca gggcctggtg ctgtggaagc cgaagggggc ggtcgtgcgc
     1921 caggagttgc agaacttcat cagcgaggag ctgcggcgtc agggctacca tcaggtcttc
     1981 acgccgcaca tcggcaagct ggggctgtat cgcacgtcgg ggcattttcc gtattaccgc
     2041 gagtcgcagt atccgccgat catcgagcac gatgaactgg cgcggctggc cgacgagggg
     2101 gcgacctgcg ccgagctgtc gaaccggatg gcggcgggga cggtggacgg ctatctgctc
     2161 aagccgatga actgcccgca tcacatccgg atcttcgcca gcgagcatcg cagctatcgc
     2221 gacctgccgc tccggctggc ggagttcggg acggtctatc gctgggagca gtcgggcgag
     2281 atcggcggga tgacgcgcgt gcggggattc acccaggacg acgcgcacat cttctgcacc
     2341 gaggatcagg tgacggccga gctgctgggc tgcctcagcc tggtgcggac ggtgtttcag
     2401 acgctgggaa tgcatgacta ccgggtgcgg gtcgggctgc gcgaccccga cagcgggaag
     2461 tatgtcggcg cgccggagaa ctgggacaag gcggaggcgg cctgccgcgc ggcggcgcgc
     2521 tcgctcggcg tcgcgttcag cgaggaaccg ggcgaggcgg cgttttacgg accgaagatc
     2581 gacttcgtcg tcaaggacgt gatcgggcgg gaatggcagc tcggcacggt gcaggtggat
     2641 tacaacctgc ccgagcggtt cgcgctggag tatatcggag cggacaacag cgcgcaccgg
     2701 ccggtgatga tccaccgggc gccgttcggc tcgatggaac gtttcgtcgg cgtgctgatc
     2761 gagcatttcg ccggggcgtt tcctccctgg ctggcgccgg tgcaggcggc cgtgctggcg
     2821 atcaccgacg cgcagttgga ctatgcccgc gaggtgctgg agcaactgaa ggaggccggg
     2881 ctgcgcgcgg agatcgacgc atcgagcgag aagatcggca agaagatccg ggccgcggag
     2941 ctggcgaaga tcccgttcat gttcgtgctg ggggcgaagg aggccgagag ccggtcggtc
     3001 gcgctgcgcc gccacggcaa gggagacgaa ggggtgctgg ggctggccga ggcggtggcg
     3061 ctgctgaagg cgaaggtgac cgagcgcgcg ttggcgtagc ggcgcgggcg cggcgggccg
     3121 attcccggac ggacccgatt gggattcccc ttgcgatatg acattgccat catcggcgcg
     3181 ggcgcggcgg ggctggcggc ggcgatcttt gccggcgcag cggcggcggc cggttccgcc
     3241 gcgcgcatcg tgctattgga cggagcgaag aagccggggg cgaagatcct cgtcagcggc
     3301 ggcgggcgct gcaacgtgac ccaccgcgag gtccggcccg aggatttcaa cggcggcccg
     3361 cagccgctga tccgcggcgt cctgcgggcg ttcgatcacc gggataccct gcgctggatg
     3421 gccgggctgg gcgtggcgct gaagctggag tcgacgggaa agtattttcc ggtgagcgac
     3481 aaggcgcgga ccgtgctcga ggcgctgctg cggcgagtcg gggagttggg ggtggagttg
     3541 cgggcggggg aaaaggtagt ggaaattgac aggcgagatt cgcatttcga atttcgtcac
     3601 tcattggaag gggagacggg cggggattcc tggttcgacc tgcaactggc gggcggcggg
     3661 gtgctgggcg tgcgccgcgt catcgtcgcg acggggggga tggcgctgcc caaatcggga
     3721 agcgatgggg cgggccttga atggatgcgc cggctggggc acgggatcat cccggccgtt
     3781 cccgccctgt gccccctggt ccttgcggac gagcagccga gcgcggcggc ggcgcgggtg
     3841 cgcgagctgg ccggcgtgac cgtcgacgcg cggctggcgc tggttgagga aaacagccgg
     3901 cgcctgggag agacgaccga ttccctgctg ttcacgcatt ttggattgag cgggccggcg
     3961 gcgatgaacc tctcgcgcca ctgggcgcgg gcgcgcgacg ccgcgccagc gacgccgcca
     4021 gtgctcgcgc tggggcatcc cagtctgccg accgaggaac gggccgatgc ctggttgcgc
     4081 gagcaggccg cggcccaccc gcggctggcc gtcggggctt gcctgcggcg gctgtggccg
     4141 gagcgcctcg cactgttgct ggcgggggag gcggccgaca cggcgctgaa cgggctgacg
     4201 cggatgcggc ggcgggcgct ggccggcgcc ctggccgggc tgcggctgcg cgttgccggc
     4261 ccgcgggggt gggccttcgc cgagacgacc gccggcggcg tcgatctgcg cgaggtcgac
     4321 atccggacga tgcagtcgcg caaggcgccg gggctgtatc tttgcggcga gatcctcgat
     4381 gtcgacggtc ggatcggcgg attcaatttt caatgggcgt ggtcgacggg atacctggcg
     4441 ggccgcggcg cggcggcgtc gttgaggggg tgaatgggac ttctggcatt cctccgggat
     4501 gccggggggg agtgaccggc gggggcgctg cgctcaacca ccggctacca tctcgcatcc
     4561 atccgggatg cggggggagc gaccggcggg ggcgccggtc ccacaccggt ggatttcatc
     4621 gcggtccgcg tccggccacc ccttccaggg atttgatgcg ctgggggcgg cgagacaggg
     4681 tcccggcctt cggcctccac cctgcgcttt attccggtcg tccgcttcgc ggactcaggg
     4741 ttcgccttgc ttcggtgacg ggagggtttg gaatggctgc gctccgcccg gggctcttga
     4801 tatggcacgc ctccggggcg acgtccggta agatttaccg tctcggggtg atgaggtggg
     4861 gaaggtttcc ccggttttac cggatggggc cggcggggcc ggtacatttt tctgacgtat
     4921 ctcgggattc ggcgccggcg ctccgatacg agagctgact caagaccgga tcaggatcgc
     4981 gagccctccg tgcccgtccc ggttcccggc gcccccgaga cgcgatgatc cgtccgcccg
     5041 tcccccgcac cccgcgcttg atccggtccg gtcttgttca cacacgagtc ttatttttca
     5101 caaaagtccg gcacatggcc ggacgataag gcatgcaggc gaaccacgga cgcagcccgg
     5161 gcccccggcc gcggcggcat cgcggccgcg gtcgaaccgg caccccgcca aggatagagg
     5221 acccatgttc aagagcaaga cgcccatcgg agtggacatc ggcagccact cggtcaaggt
     5281 ctgccaactg cgccggaccg gcgacagcta cgagctggag cgtttcggga tggcggacat
     5341 ctatccggaa ggggagccgc cgcgcgacgc cgcggggcag accgaggcga agaccgaggc
     5401 gctcaagcgg gcgctggcgt cggccgggat caagtcgcgc caggcgatca gttcggtctg
     5461 cggcgagtcg atcatcgtgc gctacctgca gcttcccgag atgccggaga acgagttgaa
     5521 gaaggcgctg cagtgggaag ccgaggaata cattccgttc cggctgtcgg aggtgaacct
     5581 ggattcggcg attctggggc gcgtgcccga cggcgagatc ccgaagatgg acgtgctgct
     5641 ggtatcggcg aagaaggacc tggtgcagcg ccatgtgagc gtgctgcgcg gggccggtct
     5701 ggagccgcgc gtcgtcgacg tcgattcatt cgcgttcatg aactgttatg aactgaatta
     5761 cgagcagggc gacgagtgca cggcgctggt gaacatcggc agcgaaatca cgagcatcaa
     5821 tgtgttcagc gcggggacgt cgcggttttc gcgcgacatc ctgatcggcg gccagacgat
     5881 cacgcaggcg atccgctcgc acctgggctg caatttcgcc gacgcggaga acctgaagat
     5941 ggcctacggc acggtgccgg cgacgggggg ggatgaggac ggcggggagg gggcgagcgg
     6001 cctgctggac acgattcgcg ggacgatcga ggaaatgacc gagcaggaaa cgagccgcga
     6061 cgcgcaggag tatgcggtgc gcaaggcggt gcaggcggtg gtgcacaacc tgctggtgga
     6121 agtgcgccgg tcgattgagt ttttcgaggg gcagtatcgc gggatcaacg tcggccgggt
     6181 gattctgggc ggcgggacgg cggggctgcc gaacctgagg gagaatgtcg aacaggagct
     6241 ccggctgccg gtggagctga tggatccgct gcggcgggtg cgggtgcccc agcgcagtgg
     6301 gttcatgagc caggccgaac agatgcgcca tcaactgggg gtggcgatcg gcctggggat
     6361 ccgcggactg gcggcctgag ggcggcgcca cgagctgaac acgcagatgc ggcgaaagga
     6421 aatgagcgac gatgatacag attaacctgc tggagaattc ctcgacgcgg caccgcgcgc
     6481 ggacggcggc ggccggcggc ggcgcggcgg gcggggccgg caccggcgcc ggcgccgggg
     6541 ggctcggcgc gatcgtctgg ctggcgctga tcctggtgct gggcgccaac ggcggcctgg
     6601 gctggatcgc gtggtccaag gtgaaggtcg ccggtgacga attccgcgcg gccgaacgca
     6661 agcagaagca gatcgaggaa cagatcgaga agcaactgac cgaggccgag caggtgcgca
     6721 agttccgtga ggtgatcaac aaccagctcg acgtgctgcg ttcgctcgat ccgcccgacc
     6781 ggatcctgtg gagccagaag gtcaacatgc tggccaacct gattccggcg aatgttttcc
     6841 tgaccgagct ggacatccag gaagacgtga cgatggtgga gacggaggcc tcgaaggccg
     6901 cgcgcgaggc gtggaagaag agcaaggcgg aggacaaggg gaaggagccg cagccggtga
     6961 tgcggccggt gatcaagtac atcgtccggc tgacgggtgt ggcgctgggc aacgacgcgc
     7021 atcagcagtt tgagaacgtc acgcgcttcc atcacgcgat gatcaatcac gtggaggagg
     7081 acgcgcaggg cgggaccgtg cgattcatgg acggcttcca gtcgaatatc gagttcgaga
     7141 aggtggaggc gacggtctac caggggacgc cgtgcaacgc gttcacgttc aagttgacga
     7201 cgcacccgat gggtcagcag gaaaacgccc cggcggcgga gaagcggcag gtggcctcgg
     7261 cgcgctaagg cgcgcgctgg gagcggccgg atccacaacc aggtttcgaa gcgaaaagca
     7321 caaggagacg tgacgatgag cgatcaacag aagacagccc tgctggtcac ggtgttcgtg
     7381 ggcctggtgg gcctgatcct gatcgcctat ttccacttca tgatcgggcg cgggatgatc
     7441 aacaagtatg agcgccggac gcgggacatg gtggaagaga cgaaggaact ggaagtgcag
     7501 ttggccgaga tccgcagcct gctgagccag aaggacgaac tggagaagca ggcggagatg
     7561 atcgagaagg tgacgcggcg gctgcccgac agcccggacg cgcccggatt cctgaacgcg
     7621 ctggtgatga ttctggggac gaccgggatc atccaggaag aggtcaagcc ggacaagaac
     7681 acggcgcgcg cgctgtatac ggagattccg tacagcgtga aggcgcacgg gcactaccac
     7741 gcgttcgggc agtttctgac gctggtggag cagaatcccg accgcttcat gcggctgaag
     7801 aagctgacga tcggcaacaa cgcggagcgt ccctcagtcc acccgatcga gatggagatc
     7861 acgacgttca tgttcaacaa ctaaggtcgc ggccggcttt ggccgatgac ctttggagac
     7921 cgaccacgag gacgagggac gagagccgat tccagagtgt gggcgagaga cggattttta
     7981 aagtgaggaa gacgagcatg aacagggaaa gggaaaggga gatgcgcacg aaccagcccg
     8041 tccgcgcgga tctagttcgc gggggcatcc gctggggcct gctgacggcg gccctgctgg
     8101 tcgccctgcc ggcgacggcc gaatcgccct ggcggcgact ggcgcgcacg ctctccaccg
     8161 agccgcaaac gacgctgccg gcggttccgc aggatgagcg cctgcggggc gtggagggga
     8221 tgacgaccgg catgccgacg ctgcccgaag tgccggacga agggacgctg atcccggcag
     8281 tgtatggcga cgcggcgacg acgggcgtgg cggcgccggc cggcggcgcg ggcgcgctgg
     8341 ccgcgttgca aggtccgaag agcgagtcgg gcaagatcag cctggggctg gagagcgagg
     8401 acgcgccggt gatggcgacc gatctattcc agcagccgga gatccgccag atgctgggcg
     8461 acgagccgcg attcgtttac aacccgaacg gccggcccga cccgatgctg ctgccctggg
     8521 tgcgcaacga ggcgatcttc aaggaactgt cggcacaggc cgaggcgctg gaggaagcgg
     8581 gcgagtatga cgcggcgatc acgctttatc agcggatcca gcggatgggc gacccgcggt
     8641 tcaacctgat cgcgcaggac aagatcatgg cgatcgcgca ggcgcaggcc gcgcaggtgc
     8701 agtcggcgat ggccgaggcg tatgtgccgg agatcaaggt ggagctgccc aactgggtgg
     8761 cggagaacac gaccggggtg atcgtcggcg aggaccgcag cctgtgcctg gtgggggatt
     8821 tcatgctggc cgagggggag gcgctgccga attatcccga ggtgcgcgtg gccgcgatca
     8881 gcgacaagct ggtgacgtat caggtgcagc acgaaacgtt tgaagtcgag ttgaagcacg
     8941 agcagtagca ggcggcgccc cagtgcccat ggaaggccga cggtgaagac cgtcaagccc
     9001 cgcttgagag ccggaatgca cgttggagag aaggagtcgt aggatggcac cgttcccgca
     9061 gcaaatcaga ctgatggcca tggccctggc gatcgccgcc acggctccct ggctgggcgc
     9121 gcaagggtca ggcgacccgg gcgggaccgc cgacgcgctc aaccagtatg tcgcccacgc
     9181 cgagaacgag gtgcgcctgg cggcggccgc gccggtcgac gtggtgatcc ccgatcccaa
     9241 cggggatgcg gaaccgcggc cggcgatgcc gggcgaggtg gcgcagaacg tcagcggcac
     9301 cggcgacgag gcgatgcagg tcaatccgga cgagacgatt gccctggact tcgaggattc
     9361 cgagcaggac atcctgacgc ggcaggcgct ggagaagctc gacgtgatcg tggagtcgct
     9421 ggagttcgcc aacgcggatc tgcgcaacgt gatccgaatc atcggcgagc ggctcgacat
     9481 caatttcatc tttgacgccg acgacatcgg cggcaaggtc agcctgcggc tgcacaacgt
     9541 gcggctgcgc gacgcgctgg agtcgatcct gtcgacgcgc aagctggcga tcgtgccgga
     9601 ccagagcggc atcttccgga tcgttccgca ggagcagatc ggccggcaac tcgtcgagac
     9661 gcggaccgag gtgattcagc tcaactggct gagcgccgac gacgtgctcg aaacgatgga
     9721 gccgttcctg accgaccatg ggcggatgat ggcgaatgag gaatccaata cgctgatcat
     9781 caccgacgtt ccgccgcaga tccaggtgat ccgcgacctg atcgcggaga tcgacctggc
     9841 cgagcgccag gtgctgatcg aggcgcggct ggccgacatt aacgtcggcg cgctgcgcaa
     9901 cctgggaacg caatggtcgg tgacgaaact gaatgaacac gcgctgcggc ggctcgactc
     9961 gcggagcatg gacaaggatt cgaccgggtt gttcgggccg gccggggtga acaatgtaga
    10021 aacggtgacg cgggatatgt atctcggcgg gaacctgatc ggttcgaccg aagtgaccgg
    10081 cctggccggc gcggtggagg cgcttgaccc gacgccgctg gccaatgccg gcgggctgat
    10141 cccggtcctg ctcgagggct tcacgctggg gggcggcctg ggctcgctct cgttcggcga
    10201 caagatcggg atcttcggcg acgaatacga tctgaacgcc acgttcagct tcctggagca
    10261 gcgcgacatc gtcgaggtgc tggccaatcc gcgcgtgacg acgctgaaca atgtgccggc
    10321 gaggatcgac atcattgaga agattcccta catcgaatcg gtgcagggcc cgaccgaagg
    10381 ggtcgtgatc ggcgagatcg agttcgagcc ggtcggcgtg acgatcaacg tcaagccgat
    10441 catcacgccc aacgggttcg tgcggatgga aatcgatctg cgccagaaga tcttccgcgg
    10501 ccgggtcgac gccggcggcg gcgggttctc ggcttatgat ccgccgcaag tggacgagcg
    10561 caacgcgatg acgaacgtca tcgtgcgcaa cgccgacacg gtggcgctgg gcggtttccg
    10621 ccaggtccgc aaggtggaag gggtcgacgg ggttccctgg ctgcaccgga tcccggtgat
    10681 cggctggctg ttcaagaaca aggagcatga ccaggccaag accgaactgg tgctgatgct
    10741 gacgccgaag atcgtggaac agcatgtcgg actgagcgat cgcgagaagt atctctacga
    10801 gaagatcgat cacgattggc acgtgccgga caagttcttc gatgaaacgc tcggcgcgga
    10861 cgaaaaggac cagcaggcgc gacgcgcggc gcgcttcggc aaggtgaact gacggctgca
    10921 aggcgaatcg gcaaatacgg cttcccgaaa cgccccacac ggcaaggccc gctcccatga
    10981 ggaagcgggc ctcgctgttt gtttgggcgg ggaaggtggg ggtggggaga gggcgtcgga
    11041 tgggatggac tgaatggacg atgtagatgg tgtggagcgg ggtggatgat gagacacgcg
    11101 cattgcgagg ggtaggattg cgctccataa tgtccgttgc gatggggccg attcaatacg
    11161 ggctttcgag agccgaggac gagagccgag agacgaattt tcagggcagg ccgttggggg
    11221 cgagggcgag gaagaggacg agggacgaga gacgattttt ttgagtgggg gcggggagat
    11281 gagcgagatc agtcgcggcg gggagcgggc gggggcggga gggccgggat cgtcggcgcc
    11341 gatcgagccg ccggccggtt caccggtcgc gccggtcggg gcggccgcgc cgtcgccgaa
    11401 gatccgcgcc gagctggaga cgctggtccg cgcccgctat ccgctgctcc agatcgtgtc
    11461 ctgggaagaa gagcgggtga tggagtgcct ggacgggatc gcgacggcgc tcggcaagca
    11521 cctgtggcag tggtcgatca acagcggcct gtgcacgtac cggcggttcg gcgggatgca
    11581 gcaggaaggg cagaagggga cgcgcgaccc gctggtggcg ctgcgcgaga tttcgaccgc
    11641 cgccgccgaa ccggggatct tcgtgctgaa ggatttccac ccgtttctgg gcgaggcgtc
    11701 ggtggtgcgc gcgctgcgcg atctggcggc gcagttgcgc acgtcatacg cgacggtgat
    11761 cctgctgggg ccgacgctgc gcgtcccggc ggaactggag aaggacctga cggtcgtcga
    11821 ttttccgttg cccgaccgcg acgacctggc cgcgtttttc gatcaactgg cgaaggactt
    11881 gcagggcaac ccggaattgc atttcgacaa caccgatgag acgcgccaac ggctgctgga
    11941 ggcggcgatc gggctgacga tgaacgaggt ggaaaacgtc ttcgccaaga tcctcgtccg
    12001 gcgcgggcgg ctgaccgtgc aggaggtgcc ggacgtctat caggagaagc gccagatcat
    12061 ccgcaagtcg ggggtgctgc aatacatcga gccgagcgaa tcgattgaga tggtgggcgg
    12121 gctggcgggg ttgaaagagt ggctgcgcaa gcggcggcgg gcgttccatc ccaaggcgcg
    12181 gcagttcggg gtgccggtgc ccaagggggt gctgttcctg ggcgtgcagg ggtgcggcaa
    12241 atcgctggcg gcgaaggcgg tcagccgcta ctggcagatg ccgctgttgc ggctcgacat
    12301 gggggagctg ttcggcagcc tggtgggcaa gagcgaggag aacgcgcggc gggcggtgac
    12361 gatcgccgag tcgatcgcgc cggtcatcct gtggatcgac gagatcgaca aggggttttc
    12421 ggggctggcc agctcgagtt tttcggacag cggcacaacg gcgcgcgtgt tcggcacgct
    12481 gctgacgtgg ttgcaggaaa aaacggcgcc ggttttcgtg atcgccacgg cgaacgatgt
    12541 caaggtgctg ccgccggagt tattgcgcca ggggcgattc gacgagatct tctttgtcga
    12601 cctgccgagc ccggcagagc ggcgggagat cttcgggatt cacctgaagc tgcgccggcg
    12661 ccggccggac cggttcgatc tggaggaact ggcacggcgc gcggagggat tttccggcgc
    12721 ggagattgag caggcggtga tctcggcgct gttcgacgcg ttcgatgagg ggcgcgacgt
    12781 cggccaggag gcgctgctgc gggcgatcgc ggcgacccgg ccgctgtcgg tgttgatgtc
    12841 cgacgaaatc gccgaacggc gcaactgggc gcggggccgg acccggccgg cgtcctgacg
    12901 cgaccgtcat tcaaacaggt cgtcgcccgg cggcagggcg gcggcgcgcc aggccccgcc
    12961 ggccgagcgg atgcggacga tctcgctgac cttgacgtcg tccgggtgct tgacgatgcg
    13021 atcgcgtttg ccgtgggtga caatgctgta gccgcgttgg aggaccgcct cggggctgac
    13081 ggcgcgcagt tgatcgtgag cggccgcaag gcgcaggcgc gcgcgttgca gatcgctccg
    13141 catcgcggcg tgcaggcggc cgcgcaggtg atcgatgcgt tcgcggttgg cgcgcagttc
    13201 ggggccccag cgcgcggggc cggtggcgtg cagtttggcg cggagccggt ccaggcgcgt
    13261 gtggagcagc tcgaggcgcg cgcgggggtt gcagcggccg agccgcaggc cggcgatcgc
    13321 gagcgcatcg cgccggtcgc gcagggtgcg ctgcatgcgg gcggtcaggt ccgccgccaa
    13381 gtcatcgagc cgctgcatca tgagctgaag gcgttcgggc gggcgccgca ggccccaggc
    13441 gtggcgacag cggtcgaggc gggcgcggcg gcgctccaga tcgcgggcga tggcgcgcgc
    13501 cagccgccgg cagaggacgg ccaggctctc ggccagttcg ccgtagtgcg ccgtgacgag
    13561 ttcggcggcg gccgaggggg tgggggcgcg caggtcggcg gcgaaatcag ccagggtcgt
    13621 gtcggtctgg tggccgacgg cggagatgac ggggatggaa caggcggcga tggcgcggac
    13681 gaccggttcc tcgttgaatg cccagaggtc ctcgatcgag ccgccgccgc ggccgacgat
    13741 gatcacctcg gcgcggccgt ggcgctggag gcgctggagg gcggcgacga tcgcgggggc
    13801 ggcctgggcg ccctggacgg ccgccggggc caggtagatc gtgatgccgc gggcgcggcg
    13861 ccccagcacg ttgagcatgt cgcgcagtgc cgcgccggtg gcggaggtga cgatgccgat
    13921 gcggcgcggg aggcgcggga tcggccgctt gcgcgcctcg tcgaacaacc cctgcgccgc
    13981 gagctggtcc ttcagttcga ggtagcggcg catcagctcg cccaggccgg cctcggccag
    14041 cgaatcgacg atgacctgat attcgctgcg ggcggtgtag gcggtgacgc ggccgcggac
    14101 ttcgagcttc atgccgtcgg cggggcggac cttctgccgc gccagggtgg agcggaaaca
    14161 caccgcgttg atcgccgtgc gctcgtcctt gagcacgaag taacagtgcc cggaggaggg
    14221 gatgcggacg ccggagactt caccctcgac acggacatag ccgatcgctt cctcgagcag
    14281 atgctggacg cgccaggaaa gttcggagac ggtcagcggg cggtcgccga gtccgttgag
    14341 ggtgggcggg ttgccggcgg tgctcatggc cgcgcctcgg gcttgcggcg aatggtcatg
    14401 agcgagatgc cgatgggcag atcgtgccgc tcaagccagg cggcctcgcc ggcgtggagg
    14461 cggatgagca ggcggttgag ccactcggcg gtttcgcgcg tgtcggagac gggggggcgg
    14521 ctggagcgcg ccagccggcg gcccagccgg atcagaaggg ccggggggaa gatcagggta
    14581 aagccggcgg tcatgcgaat cgtctcccac ggcgccgcgt tgaagagcgc gttgaactgg
    14641 gcgcggcgat agcggcgctg gtgatgcagc gagacgtcgt gggcgctcca gaggatgggg
    14701 tgcgccggga cggtggcgat gaagcagccg ccggggcgca ggacgcgcca ggcctcggcg
    14761 acgagggcga cgtcatcggc cacatgctcg atcagatcga aggcggaaac aacgtcgaag
    14821 acggcatcgg cgaaggggag gcgggcggcg tcggcgcggg cgaggcggcc cggaacgcgc
    14881 gtgcggcagc aggcgagggc ttcgaccgag aaatcgatgc cgaacggctc gcactcccgc
    14941 aggcggcgct ggaggaacag gccggtgccg cagccgaggt cgagcaggcg cgggcgcgcg
    15001 ggcaggacgg gcagcacgtg gcggcgcagc agccgctcga tcatcatcag ccggccctgg
    15061 aaccaccagt ggcggtcctc cagggcgaac atgcgctggt attcttcgtg ttccatgact
    15121 tcgcgcgcga gatggggccg gcgaggcgcg ccgccggtgt tgctgcctca aggcaatatg
    15181 aacgcaaaag cgtgtcataa tcatcgggcg ggcgcaagaa ccgcggacga atcgggatca
    15241 ggaacagaat cgacggtggt ttggggcgaa gacgagctgc ttcacgacgg ggggggaggc
    15301 tgttatgatt tgcgcggagt cgtgggctgg gattcctttg ggaaatgagg gaagggtggg
    15361 ccggcgaggg cgccggtccc acaacggctg gcggaagcga gtcgcgcttg ctccgatctc
    15421 aacctcaggt gtcgaacatg acgctgcgca ttgtcgccga tgaaaacatg ccgttcgccg
    15481 cggagcactt tggccggctg ggcgaggtaa cgctgttggc cggccgccgc atcacggcgg
    15541 acgatgtgcg cgacgccgac ctgctgatgg tccgctcggt gacgcgcgtc aaccgtgaac
    15601 tgctcgcggg ctcgcgcgtc cggttcgtcg gcacggcgac gatcgggacg gaccatgtcg
    15661 atctggatta cctgcgggaa agcggaatcg ggtttgcctc ggcgccgggg tgcaatgcgg
    15721 aatcggtggc gcagtatgtc gccgcggcgc tggcgttcgc ggcggggcgg ctggggctgc
    15781 ggctgagcga atgctcgctg gggatcgtcg gggtcggcaa ctgcggttcg cgcgtaaggc
    15841 gcgtggcgga ggcgctcggt ctgcgcgtcg ttttgaacga tccgccgctg gcgcgggcga
    15901 cgggcgattc gagctaccgg ccgctggatg agttgatgga gtgcgatttc gtgacgttgc
    15961 acgtgccgct gacgcgcggc ggcgacgacc cgacgtggcg gttgttcgac ggcgagcgca
    16021 tcgcgcggct caagcccgcg gcagtgctga tcaacgcgag ccgcgggggc gtgatcgacg
    16081 agggggcctg ggcggccggg ctgagcgacg gcccgcgcca caagctgatt ctggacgcgt
    16141 gggaggggga gccgtcgatc aaccccgaca tgctgcgccg cgcgctggtc ggcacgccgc
    16201 atatcgccgg ctattcatac gacggcaagg tggccggcac gcggatgatt tacgaagcag
    16261 cgtgccgctg gctggggcgg gaggccgcgg cgcccgcctt ggcgatgccg cccgccgccg
    16321 tgccggcgat ccgactgaac gccgcgcacc gggcggcgga ttcgatcctg gcggaactgg
    16381 tgctgacggc gtatccgatc ctgcgcgacg atgccgattt gaggacggcg ctgcacgcgg
    16441 ccggggaggc gatcggcgcg gcgtttgatg aacggcgcaa acgctacccg ctgcgccgcg
    16501 agttcgccgc gacgacgttg acgctggagg gcgccgacga agacctggcg gagcgggtgc
    16561 ggatgctggg gttcacttgc cgcaacgccg cagcgtcccg ctaagccgag tgcttccgat
    16621 ctgcttccac cgcttcacgc ggaattcacc gcctgggccg gccggcctcg cggagcgact
    16681 tgttcgcgat cctgccggca ggtattggat caagcatcgt cttctcgctt tcgtcccgga
    16741 cgaacgcctc atagcagggc gggggcgagg gcattaacgg ttgatcttca acccattttg
    16801 acctaaacaa aactaaggca gaaatcatat tcgtcgtttg accggtcggc ggccgggacg
    16861 ccggattttt tcatccgagg aaggcaggtt catcatggaa tccctccggt cgccggttgg
    16921 cgtgcgcccg cgcgccctgg cgtggctgat gctcgggatg ctgctggcgc cgcgggcgca
    16981 gccgcagccg acggagctgc gcgagaaact ttactgctgt gatccgcaac ccgagttgct
    17041 ggatctttcg ggaacctacg aaggctacta tcccgacggc gtcaagctgt tccgcagcgt
    17101 gctggagcac aatgcgcggc gcgcgtacgg catgcgcgac ttcctcacgc tctcgctcgt
    17161 cgaccggatg cagaccgaga cgggcgagct gcagatgtat accggccagc gccagatcag
    17221 ctaccggcca ttgttcggcg cgaacggggt gtgcgagggg gggctgttcg ccgagtgcgt
    17281 ccgccgtccg atcacgatca actacacggt ctcggggacg gttgaatggc tgtttacgcc
    17341 ggagggggcc gcggccaaag cgattcatct ggaagggctg atcgccggcg cgaccgagac
    17401 gacactggac tacgggacgc gcatccgggt ggcctacacg cgcggcgaca gcgcgaaccc
    17461 gcagaacgcc gtcgtgtggc gcgatctgcg cgggggcgtg ctgctgcaga tcgttttcgg
    17521 catggcgcag tttcccgagc cgggattcga tggcggctgg gaatgggtgg agacgttcga
    17581 caacccgctg caggactgcc cgtgctcgct gagcgacggg accaaccggc cgcagacgat
    17641 gtattaccag cgggtcagca gtctgccgcc gctgcaggcg ggggcggaca tccgaatgac
    17701 gctcgaggcg gcgcagccgg tcgagccggt cggcggggaa ttccggcccg agggggagat
    17761 cgtgctgctg cgccagccgc gctacgtgag ggcgcgcggg gcggaggaat cggtcaacga
    17821 ttacaacgcc tttctggcga acgaacgcca ggcgatggaa acgacagcgc accgcgcgac
    17881 gcgcgacggc gagatctacc gtttcgccga cgtgccgttc tttcaggccg gcggcgacat
    17941 cctgcagccg gcctactata cgatcgcggc gctgaacacc gagattgagg aagccatatt
    18001 cgacggcacg agcacggtca cgcagacgac gcatttcatc acgg
//
LOCUS       DAOPCO010000049        17869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3246_length_17869_cov_5.289940, whole genome shotgun sequence.
ACCESSION   DAOPCO010000049 DAOPCO010000000
VERSION     DAOPCO010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 17869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17869
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3246_length_17869_cov_5.289940"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLS90_05615"
     CDS             <1..1191
                     /locus_tag="PLS90_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01915.1"
                     /translation="SPGFAAGDTSMANIAFETNGEFQARFWQDIALADIIGDGVPTLI
                     SHSIDLYRTATGSANSQRHLGYGAQDIYFHRRDDGSTPFGSGSHSADKEIARTTTEEG
                     PLAVGDVTGDGEPELIWAENIWNRSPGWGHQFTLYVASNNGGAAGGAYDADDRSEYYD
                     EQPTQMYPNGTPTLVLAVNEFLYGASYGQFGVTAMVAGQDITRDGTPDIVVARHDDFY
                     GVDYGAANGMDDTDDLGVLQVLQISSATGALAANTTFAFDTEDFGTYKWTHLASADVN
                     CDGEVDLVAVAENTDTGNWHLFLYEWDQGGFVAGDGPGSNGWPTDDTFVPPDAGSLLN
                     SATRTDLIPASSDAIVALAVGSADGDADDEIVYSLGNGEIYVLDICGDLKEVPVNLSD
                     FLLE"
     gene            1312..2895
                     /locus_tag="PLS90_05620"
     CDS             1312..2895
                     /locus_tag="PLS90_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01916.1"
                     /translation="MSYRRILTLFTGLLLAAAARSQAPELIASDANRKVVAIELGDFA
                     QNNQGGLFAAWVHNDPAEQSLIRFYQNDGGWAHEQIAAFTNVGSNPEGTPTPPILMVA
                     GDYSNDGKTDLSVAVNSFETTTSLRHYQRNAAGFSPNDDSEANILVENGAGRQSIFWQ
                     GIAIGDLLGDGVQTLVTSDINYDQPWPGHANPIRSLGFGPQRIYLHRRAGDGAFGTQT
                     SVSDLEFGRENTEETTIAVGDITGDGEPDLFIVENLWNRSPGWGHTLTCNVMSANGGT
                     DAGTFKPGDEAEYFDDPIEFVPNALLKVGIVIHELLWDFPHGQMGLTAMAIAGTDDLT
                     RDGKADMAVARFDSFDGELYGNPPWPATGETNNLGVLEIRQTPANGAFAPVQFLEFDI
                     EDFGPYKWTILDVADVTGDGHQDLLAVAQNVNTGMWHLWRYQWDKGGFISQNGAPSAA
                     FPGDGNTYVPPSNAALLNSASKDDVIPASSTAITAMAAGNVDSDPDIEIVYALENGEL
                     YLIDGPGDQPNPARHWILY"
     gene            2968..3552
                     /locus_tag="PLS90_05625"
     CDS             2968..3552
                     /locus_tag="PLS90_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01917.1"
                     /translation="MKRWHETAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVSRC
                     LADQRSVAVGFEAYHVDNQKYPPIGTPPGFGGWAGWWLDSCYQLTTPIAYLSSTESMI
                     DPFEKPNQWAESNPYKTNSYIHYDLADTSKGMSPIYKQKSTYYLIQSFGPASLLVTDQ
                     GAFGFVPYDATNGTVSLGQIMYYGPGGQRYFRAQ"
     gene            3665..4246
                     /locus_tag="PLS90_05630"
     CDS             3665..4246
                     /locus_tag="PLS90_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01918.1"
                     /translation="MHHRPFAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVSRCL
                     ADQRSVAVGFESYHVDNQDYPPLGVPNGFQTWAGWWLDACYYLTTPIAYLSSTEAMLD
                     PFEKPPHWSETDIYKTNSYIHYDLDGGRGMSPRFAEKGLAYLVQSFGPASIMVTDNGA
                     AWGFVPYDATNGTVSLGQIMYYGPGGLRALRSN"
     gene            4273..5922
                     /locus_tag="PLS90_05635"
     CDS             4273..5922
                     /locus_tag="PLS90_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01919.1"
                     /translation="MIGSFHQSLIPLWRALATLRGASRRRPCVRFACGAAFASAAALP
                     AQPSPQLIQSDPNRTVAAIALGDFSQTGAGGVFAAWVHDDPSAESLLRFHQRAGEAWA
                     REQIAAFTDVGTTPTDSPTPPILLAAGDYSNDGKTDLTVAVNSFNTTTSLRHYQRANA
                     GFSPDDDSEANVLTETGAGRRSTFWQALAIGDLLADGVPTLVSSDINFFEPYAGHAAP
                     LPALGFGPQRITLHRRAGAGAFGAQSSVSDLEYARENSEETTLAVGDISGDGFPEVLI
                     VENIWNRSPGWGHQLSVAAMSANGGDDAGSFVAGDVEDFFDDPVEFAPNALLSVDRVI
                     RELFWSDAHGQMGVTAVAIAGADDLTRDGIADLAVARFDSYDGEPYGNPPFPATAATN
                     GHGVLEVRQVPFNGEFLGEDLRFDIEDYGPWRWTSLHVADTDGDGAPELIAAAEDLES
                     GLWHLWRYVWDKGGFINNDGAPSAAFPDGDYHPPAPAALLNSATRTDIIPPAGAAITA
                     LAVGNADADDDVEIVYGLDNGELYVLDEAGGVNAARSWMLF"
     gene            6050..7252
                     /locus_tag="PLS90_05640"
     CDS             6050..7252
                     /locus_tag="PLS90_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPK01920.1"
                     /translation="MTSPRQETLDRPAPALPSRIAALPRVGSRVTDQRIAQLKAQGRE
                     ILPLTAYPHRELPESALAAGRAALDRLDHPPARGLRGLRAALADYWGRLTAHAYDPDR
                     EVLITAGTMHALMTCFMAILDPGDEVLLPTPAYFFDGSVVLAGGVLRFVPFDEADDFR
                     WRPERLEAAVGPRTRAILLNTPTNPTGAVASRDDLEQIAAIAEKHDLYVICDESYDRL
                     VYDGGRHVSLLECETARDRLILAASFTKSFAMANWRVGYLIGPPPVMDAALKALEWSF
                     LYGPLVNQCVAEAVLRHDLSWLDGVGAEFQANRDRLCAALAADPRLSFVRPRGNPFIF
                     LNVSRLGDDARVASHLLEHHGLPCTAGHLHGAPGYLRVAFGGPPAAVDEAARRILRAA
                     DELAGDNS"
     gene            7295..8179
                     /locus_tag="PLS90_05645"
     CDS             7295..8179
                     /locus_tag="PLS90_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HPK01921.1"
                     /translation="MKTLLEDQYIIEFDPAAAPAIELEPGEELEARVKDWCFGLVSDD
                     PATYERASRTPRCPAAGPVFVRGAAPGDAIAVRILALDPISPGHMVIRPGCGPFGDRV
                     EQMEILRIPLEDGMARLPTGLRVPLAPMLGVLGATPADAPAPTLISGDHGGNLDTREI
                     AAGSTVLLPVQVEGGLIAFGDAHAVMGDGELSGSGVEVAVVARLAFDVLPGRNLRRPR
                     ILSPTHMITLATRDDFRAAVELATNDMADWIIELTGWSFRDAALLIGAAGRLLVSHVI
                     NRPGPTIKLALSRELLQI"
     gene            8209..10587
                     /locus_tag="PLS90_05650"
     CDS             8209..10587
                     /locus_tag="PLS90_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01922.1"
                     /translation="MRTPLVSTQSQTVTFGLESPSRWIHFIILAALLALVPIAGPAQA
                     PESSDDRPRLENPSLALVFDPRDGSLAAVTNRLTGERHDVAAQAFLLYTDQGEISPAT
                     APLAATASIENGIRFTYRHPSAAIELDYRLHPEHHFVEKIIRLKNESDQPILIQEIIA
                     ERFRFTPAFAEVYAHDDGTDWRCPINVFLRTRKGGLFTGVENPYFEMIVPGAWQATRL
                     DLRFAPRWILHPGETFEAEPSFLGAYRNEGIYFFKELQPFAYMSNQQIIMDWGEVWAM
                     QEFLRDIMPPKELPYPDYYMRANAVNYFTNLGRARGTEEKPFGKDREWWPPHSHAPWE
                     PAMTEAGKHYIDMLKKQGNIDSIDWGTIWLGHAGWLRESPDEYLERLGTDWDIEPNPH
                     WLELIRYGQQQGFGVGIMDEGGARDYLKYEDHWKVKDKSGKPIQGVAQLEGYMGRNCW
                     ANPEFANWWVDIVSKTIDEYDVPIWGTDAGSIWWVPFDPPLECYDPDHGHPVGECAYY
                     AWRNIMNASAELVRRHPDCVLRNAGGMHRGYPWVLRDWIEYHGYLDPVAQGEGGTVND
                     NIRFQGWHARNLRFIPGYKSGGGLRTDDPYGMAYGAFTHMTHSDHGEAEMVPPVGVTD
                     PAEQQRHVDFFRKWAQWADDNVQFMRVRRDILGEPRTSKLDGSAHCIGDRGFLFVFNP
                     GRRARAAEIPINHWIGLTEGDAFTISYIYPDDGGPVGVYQRDERLLIPVPSHEVVALS
                     IQPADAEGSSPDARPDVSGLPVDKAFHDAADVRERFGDDIRLPPSARPADED"
     gene            10643..12463
                     /locus_tag="PLS90_05655"
     CDS             10643..12463
                     /locus_tag="PLS90_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HPK01923.1"
                     /translation="MLDRRTFLGELGLGGLGLLAPPARAEPDPAARFHRPERPWPAFT
                     TRPVLMARRDVVTAGHYLAAAAGWQMIAAGGNAFDAAVAMGFASAVLEPQYFGLGGEA
                     SILLYSAERNKALALSGQGVAPRAATIDWFRAHGIDSIPGTGFLPLTIPACPAAYFEL
                     LRQFGTLPAHEVLAPALDLAENGYPAHLGAINAIHGASAVYPTTRRAYTPAGGQYEVG
                     ELIRIPGLAATFRALIDASRVQPTRERGIAAAHDAFYQGPIARRMAEFATTEASGFYE
                     GIPGLLSVDDLAAFSASVEEPVRARYRGYDVCKCGPWSQGPVFLQQLKLLEGFDLRAL
                     GHNSADYLHTLIEAAKLAFADRDAFYGDPNFVDVPLDRLLSEPYADERRALIDPNRAS
                     LDIRPGDGAPLPAPAVHGLPDDTTHLDAIDRHGNMVAITPSGGWITHSPVIPDLGFSL
                     GVRGQMFWLDPAHPNRLQPGKRPRTTLSPTLVMRDGEPLMVFGTLGGDAQDQWTLQFF
                     LNYIDFGMNLQEAIDAPTVTTYHFHQSWAGHSVDSGKLTAEDRIDPRVIEELTARGHN
                     VQLVGDWSHGRVLAAAVDSRAGIRRAAASPRAETGYAYGW"
     gene            12601..13689
                     /locus_tag="PLS90_05660"
     CDS             12601..13689
                     /locus_tag="PLS90_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HPK01924.1"
                     /translation="MARQPAFFDVFSFERLPTPPADCRLIGGEADPALDYLVGPAFFD
                     PQVNGYAGVDFQDPDVTLDALEHAAEQLARAGCPHFLLTIITGPDDFLRDHFARLAGF
                     LERSERLRRHIPGFHLEGPFLNDNPGYRGAHRPELMTDPQWDRFAAWQAASGDRIRML
                     TIAPERAGAVDFIRRAVAAGVFLCAGHSAATRRDLLAAVQAGLRLYTHLGNGAPLEIP
                     RHANVIQDVLSVPGLAASLIADGIHLPPYVLANFARALGSARLVLTTDAMAAAGAPTG
                     RYALAGIALEVGDDRVVRNPNAPGFAGSALSPLEGFYNGIQFAGLGAAASWHAWTWLR
                     RELFPDLAPPRIALPFPAPPSSRHRLGL"
     gene            13933..15756
                     /gene="aspS"
                     /locus_tag="PLS90_05665"
     CDS             13933..15756
                     /gene="aspS"
                     /locus_tag="PLS90_05665"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPK01925.1"
                     /translation="MLAPLESQRRSCYCGDVRTGHIGQQIILKGWVQNHRDHGGVYFV
                     DLRDRSGLVQVVFDPEELPAEAFERATRLHYEDVLAVRGTVRHRPEGTVNPRLKTGEV
                     EVLLSDYAILSESAPLPFPIDEFTQSAEDLRLRYRYLDLRRPRMQQTLIERARVNGII
                     RRALDAEGFIEIETPVLTRSTPEGARDFLVPSRLNPGTFYALPQSPQLFKQLLMVAGM
                     DRYYQIVRCFRDEDLRANRQPEFTQLDIEMAFVTPDELFEIMERMLTAVFRDVKGIDL
                     KTPFDRMTYAEAMNRYGSDKPDRRFKLELHDITAAVRAGGCEFKVFVDLLGQGGVLRA
                     LVVPGGGEKFSNTQLKPGGELPTFAERHGARGLAWFRTVQDDSAVRLESSISKFFDAA
                     CQARIIEATGAGAGDLILIVADQPIASAQALGQLRLKLGRDLGLIPNDEFNFCWITDF
                     PLLEWNADEKRWFSMHHPFTMPHPEDWDKLESDPGAVRSLAYDLALNGEELGGGSIRI
                     HRRDLQERAFRALGIGEDEARMKFGFLMDAFQYGAPPHGGIAFGMDRLMMLLLGLDSI
                     RDVIAFPKTQTGACLMTSAPAEVDEAQLRELSIRSTARKEK"
     gene            16144..16695
                     /locus_tag="PLS90_05670"
     CDS             16144..16695
                     /locus_tag="PLS90_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPK01926.1"
                     /translation="MAQLSRDRSFLMVIDLQERLLPAIHDHQAVRDRSVFLIRAARIL
                     GLPIVWTEQYRKGLGPTVPPVAEAIGDAARPMEKMAFGCLCDDAVARAVESAGRDQLI
                     LCGIEAHVCVLQTALAARERGLTVFLAEDAIGSRRPSDRDAALRRMAQAGCVPATSEM
                     LVMEALRVAGTDPFKQLLPLLKG"
     gene            16799..17521
                     /gene="bshB1"
                     /locus_tag="PLS90_05675"
     CDS             16799..17521
                     /gene="bshB1"
                     /locus_tag="PLS90_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HPK01927.1"
                     /translation="MIDIVAFGAHADDAELFAAGTLALAARRGRSTAIVDLTRGEMAT
                     RGTPETRRREAQAAAGILALTHRETLDLGDGRLTNDHASRVLVASVIRRLRPTLVLTH
                     GREDRHPDHRAAHDLVRDAAFFAYVGEFPADGERWRPAGLAFYLGNSFQPEARADWVV
                     DISATYEIKMQALRAYASQFLADPAQPDRATYIASPEFWDAIERRSRLWGHRIGVAHG
                     EPFLLDRPASALHPLVQLLPRP"
BASE COUNT         2877 a         7296 c         4858 g         2838 t
ORIGIN      
        1 agccccggct tcgccgccgg cgatacctca atggccaaca tcgccttcga gaccaatggt
       61 gagttccagg cccgcttctg gcaggacatc gccctggccg acatcatcgg cgacggcgtc
      121 ccgaccctca tctcccactc gatcgacctg taccgcaccg ccaccggctc ggccaacagc
      181 cagcgccacc tgggctacgg cgcgcaggac atctacttcc accgccgtga cgacggctcg
      241 actccgttcg gctccggctc ccactcggcc gataaggaaa ttgcccgcac caccaccgag
      301 gaaggccccc tcgccgtcgg cgacgttacc ggcgatggcg agcccgaact catctgggcc
      361 gagaacatct ggaatcgttc gcccggctgg gggcatcagt tcacgctcta tgtcgcctcc
      421 aacaacggcg gcgcggcggg cggcgcctac gacgccgacg accggagcga atactatgac
      481 gaacagccca cccagatgta ccccaatggc acgccgaccc tcgtcctcgc cgtcaacgaa
      541 ttcctctacg gcgcctccta cggccagttc ggcgtgaccg ccatggtggc cggccaggac
      601 atcacccgcg acggcacccc cgacatcgtc gtcgcccgtc acgatgattt ctatggagtc
      661 gattacggcg cggccaacgg catggatgat accgacgatc tgggcgtctt gcaggtcctc
      721 cagatcagca gcgccaccgg cgccctcgcc gccaacacca ccttcgcctt cgacacggaa
      781 gacttcggca cctacaagtg gactcacctg gccagcgccg acgtcaactg cgatggcgaa
      841 gtcgatctgg ttgccgtcgc cgagaacacc gacaccggca actggcacct gttcctctat
      901 gaatgggatc agggcggctt cgtcgccggc gacggtcccg gcagcaatgg ctggccgacg
      961 gacgatacct tcgttccccc ggacgccggt tcgctgctca acagcgccac ccgcaccgac
     1021 ctgattcccg ccagcagcga cgcgatcgtc gccctcgccg tcggcagtgc cgatggcgac
     1081 gccgacgacg aaatcgtcta ctcgctgggc aacggcgaaa tctacgtgct ggatatctgc
     1141 ggcgatctga aagaggttcc cgtcaacctc tccgacttcc tgctggagta gtccggatcc
     1201 cccttcaggg gcggccgtca agggcggccg cccctgactt tccccttttc gaaaccgccg
     1261 gaccaatgcc ggcggttgga tgcataccac ggacaaggag tttcaggaca aatgagctat
     1321 cgacgcattt tgacattgtt caccgggctg ctgctcgcgg ccgccgcgcg ctcccaggcg
     1381 cccgagctga tcgcctcgga cgccaaccgc aaggtggttg ccatcgagct gggcgatttc
     1441 gcccagaaca accagggcgg cctcttcgcc gcctgggtcc acaacgaccc cgccgagcaa
     1501 agtctgatcc gcttctatca gaacgatggc ggttgggccc atgaacagat cgcggccttc
     1561 accaacgtcg gctcaaaccc cgagggcacc cccaccccgc cgatcctgat ggtcgcgggc
     1621 gattacagca acgacggcaa gaccgacctg agcgtcgccg tcaactcctt cgagacgaca
     1681 acctcgttgc gccactacca gcgcaacgcc gccggcttct cccccaatga cgacagcgaa
     1741 gccaacatcc tggtcgagaa cggcgccggc cgccagagca tcttctggca gggcatcgct
     1801 atcggcgacc tgctcggcga cggcgtccag accctcgtca ccagcgatat caattacgac
     1861 cagccctggc cgggtcatgc caacccgatt cgctcgctcg gtttcggccc gcagcgcatc
     1921 tatctgcatc ggcgcgccgg cgacggcgcc ttcggcaccc agaccagcgt ctccgacctc
     1981 gaattcggcc gtgagaatac cgaggaaacc accatcgccg tcggcgacat cactggcgac
     2041 ggcgaacccg atctgttcat cgtcgagaac ctctggaacc gctcccccgg gtggggccac
     2101 accctcacct gcaacgtgat gtcggccaac ggcggcacgg atgccggcac cttcaagccg
     2161 ggcgatgagg cggagtactt cgacgatccg atcgaattcg tccccaacgc cctgctcaaa
     2221 gtcggcatcg tcatccacga actcctctgg gatttcccgc acggccagat gggcctgacc
     2281 gccatggcca tcgccggcac cgacgacttg acccgcgacg gcaaggccga catggccgtg
     2341 gcccgcttcg acagctttga cggcgagctc tacggcaatc cgccgtggcc ggccaccggc
     2401 gagaccaaca atctgggcgt cctcgaaatc cgtcagaccc cggccaacgg cgccttcgcc
     2461 cccgtccagt tcctcgagtt cgatatcgag gatttcggcc cctacaagtg gacgatcctc
     2521 gatgtcgccg acgtcaccgg cgacggccat caggacctcc tcgccgtcgc ccagaacgtc
     2581 aacaccggca tgtggcacct ctggcgctac cagtgggaca agggcggctt catcagtcaa
     2641 aacggcgccc cctcggccgc attccccggc gacggcaaca cctacgtgcc gccgtccaat
     2701 gccgccctgc tcaacagcgc ctccaaggac gatgtcatcc ctgcttccag caccgccatc
     2761 accgcgatgg ctgccggcaa cgtggatagc gaccccgaca tcgaaatcgt ctatgcgctc
     2821 gaaaacggcg aactctacct catcgacggg cccggcgatc aacccaatcc cgcccgccac
     2881 tggatcttgt actgaagctt gaacaccaat cgcagctccc cgccccgggc gggggcccaa
     2941 actccaaact tagggaggaa atcacccatg aagcgctggc atgagaccgc tttcaccctg
     3001 atcgaattgc tcatcgtcgt cgcgatcatc gccatcctgg cggcgatcgc cgtccccaac
     3061 ttcctcgagg cgcaaacccg ctccaaggtc tcccgctgcc tcgccgatca acgctccgtc
     3121 gccgtcggct tcgaggccta tcacgtcgac aaccagaaat atccccccat cggcacccct
     3181 cccggcttcg gcggctgggc cggctggtgg ctcgatagct gttatcagct cacgaccccc
     3241 atcgcctacc tctccagcac cgaatccatg atcgatccat tcgaaaagcc caaccagtgg
     3301 gcggaatcca acccctacaa gaccaactcg tacattcact acgacctggc cgacacgagc
     3361 aagggcatga gcccgatcta caagcagaaa tccacgtatt acctgatcca gagcttcggc
     3421 cccgcctcgc tcctcgtcac cgaccagggc gccttcggct tcgtccccta cgacgccacc
     3481 aacggcaccg tcagcctcgg ccagatcatg tactacggtc cgggagggca acgctacttc
     3541 cgggctcagt aaccctcacc cccccaacac gctccgccgg ccggcgatcc gtccctgatc
     3601 cccggccggc ggagtcccca tgccggcgct tgcctggcct tcgatctcag cgggagaaat
     3661 cagcatgcat catcgcccct ttgccttcac gctgatcgaa ctgctcatcg tcgtcgccat
     3721 catcgccatc ctggcggcga tcgccgtacc caacttcctc gaggcccaga cccgctccaa
     3781 agtctcccgc tgcctcgccg atcaacgctc cgtcgccgtc ggcttcgaat cctatcacgt
     3841 cgacaaccag gactatcccc ccctgggcgt ccccaatggc tttcagacct gggccggctg
     3901 gtggctcgac gcctgctact acctgacgac ccccatcgcc tacctcagca gcaccgaggc
     3961 gatgctcgac cccttcgaga aaccgcccca ctggtccgag accgacatct acaagaccaa
     4021 ctcctacatc cattacgatc tggatggcgg ccgggggatg tccccccgct tcgcggagaa
     4081 gggcctcgcc tacctcgtcc agagcttcgg acccgcctcg atcatggtca ccgataacgg
     4141 cgccgcctgg ggcttcgttc cctacgacgc caccaacggc accgtcagcc tcggccagat
     4201 catgtactac ggcccgggcg gcctgcgcgc gttgcgctcc aactgatccg cggcgttcaa
     4261 ggagcagctc cgatgattgg ttccttccac cagtccctga tccccctttg gcgcgcgctg
     4321 gcgacgcttc gcggcgcctc gcggcgacgc ccctgcgtcc gcttcgcttg cggtgcggcc
     4381 ttcgcctcgg cggccgccct gcccgcccag ccctcgcccc agctcatcca gtccgacccg
     4441 aaccgcaccg tcgccgcgat cgccctcggc gatttctccc agaccggcgc cggcggcgtc
     4501 ttcgccgcct gggtccacga cgacccttcg gccgagagcc tcctccgctt ccatcagcgc
     4561 gccggcgagg cttgggcccg cgaacagatc gccgccttca ccgatgtcgg caccacgccc
     4621 accgattcgc cgacccctcc catcctgctc gccgccggcg attacagcaa cgacggcaag
     4681 accgatctga ccgtcgccgt caattccttc aataccacga cttccctgcg ccactaccag
     4741 cgcgcgaacg ccgggttttc gcccgacgac gactccgagg cgaatgtcct gaccgagacc
     4801 ggcgccggcc gccggagcac cttctggcag gccctcgcga tcggcgacct cctcgccgac
     4861 ggcgtcccga cccttgtcag cagcgacatc aacttcttcg aaccctacgc cggccacgcc
     4921 gccccgctcc ccgcgctcgg tttcggcccg cagcgcatca cgctgcatcg ccgcgccggc
     4981 gccggcgcct tcggcgccca gagcagcgtc agcgacctgg aatacgcccg cgaaaactcc
     5041 gaggagacga ccctggccgt cggcgacatc tccggcgatg gattccccga agtcctcatc
     5101 gtcgagaaca tctggaatcg ctcgcccggc tggggccacc agctcagcgt cgccgcgatg
     5161 tcggccaatg gcggcgacga cgccggttcg ttcgtcgccg gcgatgtcga ggacttcttc
     5221 gacgaccccg tcgaattcgc ccccaatgcc cttctcagcg tcgatcgcgt catccgcgaa
     5281 ctcttctgga gcgacgccca cggccagatg ggcgtcaccg ccgtggccat cgccggcgcc
     5341 gacgacctga cccgcgacgg catcgccgat ctggccgtcg cccgcttcga cagctacgac
     5401 ggcgagccct acggcaatcc cccgttcccc gccaccgccg cgaccaacgg ccacggcgtc
     5461 ctcgaagtcc gccaggtccc cttcaacggc gaattcctcg gcgaggatct gcgcttcgac
     5521 atcgaggatt acggcccctg gcgctggacc agcctgcacg tcgccgacac cgacggcgac
     5581 ggcgcccccg aactgatcgc cgccgccgag gatctcgagt ccggcctctg gcacctctgg
     5641 cgctacgtct gggacaaggg cggtttcatc aataacgacg gcgccccctc ggccgccttc
     5701 cccgatggcg actaccatcc ccccgccccg gcggccctgc tcaacagcgc cacgcgcacc
     5761 gacatcattc cccccgccgg cgcggccatc acggccctcg ccgtcggcaa tgccgacgcc
     5821 gacgacgatg tcgaaatcgt ctacggattg gacaatggcg agctttacgt tttggatgag
     5881 gccggcggcg tcaatgccgc ccgctcctgg atgcttttct gagcgcccgg cctcgtcccg
     5941 gcggcggtgc tccgccggcc cgacggctcc atccgagccg ccgacctcga ccccatgccc
     6001 tgaagggagc ccctgcgcgt gatcctggat cgaaaggatg tgccggacca tgactagccc
     6061 acggcaggag acgctcgacc ggccggcccc cgctttgccc tcccgcatcg ccgccctgcc
     6121 gcgcgtcggc tcgcgcgtca ccgaccagcg catcgcccag cttaaggctc aggggcgcga
     6181 aattctcccg ctgacggcct atccccaccg cgagctgccc gagtccgccc tggccgccgg
     6241 ccgcgcggcc ctcgaccggc tcgaccatcc ccccgcccgc ggcctccgcg gcctgcgcgc
     6301 cgccctcgcc gattactggg gccggctcac cgcccacgcc tacgaccccg accgcgaagt
     6361 cctcatcacc gccggcacga tgcacgccct gatgacctgc ttcatggcca tcctcgatcc
     6421 cggcgacgag gtcctgctgc ccacccccgc ctatttcttc gatggcagcg tcgtcctcgc
     6481 cggcggcgtc ctgcgctttg tcccgttcga tgaggccgac gacttccgct ggcgccccga
     6541 gcggctcgag gccgccgtcg gcccccgcac ccgcgccatc ctgctcaaca cgccgaccaa
     6601 tcccaccggc gccgtcgcct cgcgcgacga cctcgagcag atcgccgcca tcgccgaaaa
     6661 gcacgacctc tacgtcatct gcgacgagtc ctacgaccgg ctcgtctacg acggcggacg
     6721 ccacgtcagc ctgctcgaat gcgaaaccgc ccgcgaccgc ctgatcctcg ccgccagctt
     6781 caccaagagt ttcgccatgg ccaactggcg cgtcggctac ctgatcggcc ccccgcccgt
     6841 catggacgcc gcgctcaagg ccctggagtg gtccttcctc tatgggccgc tggtcaatca
     6901 gtgcgtggcc gaggccgtcc tgcgccacga cctctcatgg ctcgacggcg tcggtgcgga
     6961 gttccaggcc aatcgcgacc gcctgtgcgc tgccctcgcc gccgatccgc gcctgtcgtt
     7021 cgtccgcccc cgcggcaacc cgttcatctt cctgaatgtc tcgcgcctgg gcgacgacgc
     7081 gcgcgtcgca agccatctgc tcgagcacca tggcctcccc tgcaccgccg gccatctgca
     7141 cggcgccccc ggctatctgc gcgtggcctt cggcggtccc cccgccgccg tggacgaagc
     7201 cgcccgccgc atcctccgcg ccgccgatga actcgcgggc gacaactcct gacccagtcc
     7261 cttatgtccc tgattccccc actgaaagcc ccccatgaaa acactgcttg aagaccaata
     7321 catcatcgaa ttcgacccgg ccgccgcgcc ggccatcgag ctcgagcccg gcgaggagct
     7381 cgaagcccgc gtcaaggact ggtgcttcgg cctcgtcagc gacgaccccg ccacctacga
     7441 acgggcctcg cgcacgccgc gctgccccgc cgccggcccg gtcttcgtgc gcggcgccgc
     7501 cccgggcgat gccatcgccg tccgcatcct cgccctcgac ccgatcagcc ccggccatat
     7561 ggtcatccga cccggctgcg gccccttcgg cgaccgcgtc gagcaaatgg aaatcctgcg
     7621 catcccgctc gaggacggca tggcgcggct ccccaccggc ctgcgcgtcc cgctcgcgcc
     7681 gatgctcggc gtgctcggcg cgacgcccgc cgacgcgccc gcccccaccc tgatctccgg
     7741 cgatcacggc ggcaatctcg atacccgcga aatcgccgcc ggctccaccg tcctcctccc
     7801 cgtccaggtc gagggcggcc tgatcgcctt cggcgacgcc cacgccgtca tgggcgacgg
     7861 cgaactgagc ggcagcggcg tcgaagtcgc tgtcgtcgcc cgcctcgctt tcgacgtcct
     7921 ccccgggcgc aacctccgcc ggccccgcat cctcagcccg acccacatga tcaccctcgc
     7981 cacccgcgac gacttccgcg cggccgtcga gctggccacc aacgacatgg ccgactggat
     8041 catcgaactg accggctgga gtttccgcga tgccgccctc ctgatcggcg ccgccgggcg
     8101 gctcctggtc agtcacgtca tcaaccgccc cggccccacg atcaagctcg ccctcagccg
     8161 cgagctcctc cagatctgac ttccactccc gcacgaaaag gacacccgat gaggaccccg
     8221 cttgtgtcga ctcaatcgca aaccgtaacc ttcgggctgg aatcgccctc ccgctggatt
     8281 catttcatca tcctggcggc gctccttgcg ctcgtcccga tcgccggccc ggcccaggca
     8341 cccgaatctt cggacgatcg gccccgcctc gaaaacccca gcctggcgct cgtcttcgat
     8401 ccgcgcgacg gcagcctcgc cgccgtcacc aaccgcctca ccggcgagcg ccacgacgtc
     8461 gccgcccagg ccttcctgct ctacaccgac cagggcgaga tctcccccgc caccgccccg
     8521 ctcgccgcga ccgcctcgat cgagaacggc atccgcttca cctatcgcca ccccagcgcc
     8581 gccatcgaat tggactaccg gctccacccc gagcatcatt tcgtggagaa aatcatccgc
     8641 ctgaaaaacg agagcgatca gcccatcctg attcaggaga tcatcgccga gcgcttccgc
     8701 ttcacccccg cctttgccga agtttacgcc cacgacgacg gcaccgactg gcgctgcccg
     8761 atcaatgtct ttctgcgcac gcgcaagggc ggcctcttca ccggcgtcga gaatccctac
     8821 ttcgaaatga tcgtccccgg cgcatggcag gccacccgcc tcgacctgcg cttcgctccc
     8881 cgctggatcc tccatcccgg cgaaacgttt gaagccgaac ccagcttcct cggcgcctac
     8941 cgcaacgaag gcatctactt cttcaaggaa ctgcagccct tcgcctacat gtccaaccag
     9001 cagatcatca tggactgggg cgaggtctgg gccatgcagg agttcctccg cgacatcatg
     9061 ccgcccaaag aactccccta ccccgattat tacatgcgcg ccaacgccgt gaactatttc
     9121 accaacctcg gccgcgcgcg gggcacggaa gagaaaccgt tcggcaagga ccgcgaatgg
     9181 tggccacccc acagccatgc cccctgggaa ccggccatga ccgaggccgg caagcattac
     9241 atcgacatgc tcaagaagca gggcaacatc gacagcatcg actggggcac catctggctc
     9301 ggccacgccg gctggctccg cgaatccccc gatgaatacc tcgagcgcct cggcactgac
     9361 tgggacatcg agcccaaccc ccactggctg gaactgatcc gctacggcca gcagcagggt
     9421 ttcggcgtcg gcatcatgga cgaagggggc gcccgcgact acctcaagta cgaagaccac
     9481 tggaaggtca aggacaagag cggcaagccg atccagggcg tcgcccagct cgagggctac
     9541 atgggccgca actgctgggc caaccccgaa ttcgccaact ggtgggtcga catcgtcagc
     9601 aagacgatcg acgaatacga cgtccccatc tggggcaccg atgccggcag catctggtgg
     9661 gttcccttcg accccccgct ggaatgctat gatcccgacc acggccaccc cgtcggcgaa
     9721 tgcgcctact acgcctggcg caacatcatg aacgcttccg cggaactcgt ccgccgccac
     9781 ccggattgcg tgctgcgcaa cgccggcggc atgcaccgcg gctacccctg ggtcctccgc
     9841 gactggatcg aataccacgg ctatctcgat cccgtcgccc agggcgaggg cggcaccgtc
     9901 aacgacaaca tccgcttcca gggctggcac gcccgcaatc tgcgcttcat ccccggctac
     9961 aaatccggcg gcggcctccg caccgacgac ccctacggca tggcctacgg cgccttcacc
    10021 cacatgaccc acagcgatca cggcgaggcc gaaatggtcc ccccggtcgg cgtcaccgac
    10081 cccgccgagc agcagcgcca cgtcgatttc ttccgcaaat gggcgcagtg ggccgatgac
    10141 aatgtccagt tcatgcgcgt ccgccgcgac atcctcggcg agccccgcac gagcaagctc
    10201 gacggcagcg cccactgcat cggcgatcgc ggcttcctct tcgtcttcaa tcccggccgc
    10261 cgggcccgcg ccgccgaaat cccgatcaat cactggatcg gcctgaccga aggcgacgcc
    10321 tttaccatct cctacatcta ccccgacgac ggcggaccgg tcggcgtcta ccagcgcgat
    10381 gaacggctcc tgattccggt cccctcgcac gaagtggttg ctctctcgat ccagccggcc
    10441 gatgctgaag ggtcctcgcc agacgcccgc cccgacgttt ccggtctccc cgtcgacaag
    10501 gcgttccacg acgcggccga cgtccgcgag cggttcggcg acgacatccg cctccccccg
    10561 tcggcccggc cggcggatga ggactgacgc gcgccggccg gccccgcccc cggccacaac
    10621 cctggcaaag gatccgcccc ccatgctcga tcgccggaca ttccttggcg agttgggact
    10681 cggcgggctc gggctgctgg cgccccccgc ccgcgccgag cccgatcccg ccgcccggtt
    10741 ccaccgcccc gagcgcccct ggcccgcctt taccacccgg ccggtcctca tggcccggcg
    10801 cgacgtcgtc accgccggcc actatctggc cgccgccgcc ggctggcaga tgatcgccgc
    10861 cggcggcaac gccttcgacg ccgccgtcgc catgggcttc gcctccgccg tcctcgaacc
    10921 tcagtatttc ggcctgggcg gcgaggcctc gatcctcctc tactccgccg agcgcaacaa
    10981 ggccctcgcc ctcagcggtc agggcgtcgc cccccgcgcc gccacgatcg actggttccg
    11041 cgcccacggc atcgattcca tccccggcac cggcttcctg ccgttgacca tccccgcctg
    11101 ccccgccgcc tacttcgagc tgttgcgcca gttcggcacc ctccccgccc acgaggtcct
    11161 cgcccccgcg ctcgacctgg ccgagaacgg ctatcccgcg cacctgggcg cgatcaacgc
    11221 catccacggc gcctccgcag tctatcccac gacccgccgg gcctacaccc ccgccggcgg
    11281 ccagtacgaa gtcggcgaac tcatccggat cccgggcctg gccgccacct tccgcgccct
    11341 catcgacgcc tcgcgcgtgc agcccacccg cgaacgcggc atcgcggccg cgcacgacgc
    11401 cttctaccag ggccccatcg cccgccgcat ggccgaattc gcgaccaccg aggcctcggg
    11461 cttttacgag gggatccccg gcctcctctc cgtcgacgat ctcgccgcgt tctccgcctc
    11521 cgtcgaggaa cccgtccgcg cccgctatcg cggctacgac gtctgcaaat gcggcccctg
    11581 gtcccagggt cccgtcttcc tccagcagct caaattgctc gagggcttcg acctgcgcgc
    11641 gctcggccac aattccgccg actatctcca caccctgatc gaagcggcca agctcgcctt
    11701 cgccgatcgc gatgccttct acggcgaccc caactttgtg gatgtcccgc tcgaccggtt
    11761 gctctccgag ccctacgccg acgagcgccg cgccctgatc gatccgaatc gcgcttccct
    11821 cgacatccgc cccggcgacg gcgccccgct ccccgccccg gccgtccacg gcctgcccga
    11881 cgacacgacg cacctcgacg cgatcgaccg ccatggcaac atggtcgcca tcacccccag
    11941 cggcggctgg atcacccact cccccgtcat ccccgatctc ggcttctccc tcggcgtgcg
    12001 cggtcagatg ttctggctcg accccgccca tcccaaccgc ctccagccgg gcaagcgccc
    12061 gcgcaccacg ctctcgccca ccctcgtcat gcgcgacggc gaaccactga tggtcttcgg
    12121 cacgctcggc ggcgacgcgc aggatcaatg gacgctccag ttcttcctca actacatcga
    12181 tttcggcatg aacctccagg aggcgatcga cgcccccacg gtcaccacct accattttca
    12241 tcagtcgtgg gccggccaca gcgtcgatag cggcaaactc accgccgagg accgcatcga
    12301 cccccgcgtc attgaggaac tgaccgcccg cggccacaac gtccagctcg tcggtgactg
    12361 gagtcatggc cgcgtcctcg ccgccgcggt ggattcgcgc gcgggcatcc gccgcgccgc
    12421 cgcctccccc cgcgccgaaa ccggctacgc ctacggctgg taatccgcct cccaataaat
    12481 cgtcatcgct cgtctctcgt cctcgtccct caaaaaaacc aacactgtaa atcccgccgc
    12541 ttcgatggca agctggtccc gacaagtacc cccaaccccc tctcctcgca ggattcggcc
    12601 atggcccgtc agcccgcttt cttcgacgtc ttctccttcg agcgcctccc tacccccccc
    12661 gccgactgcc gcctcatcgg cggcgaagcc gaccccgccc tcgactacct cgtcggcccc
    12721 gccttcttcg atccccaggt caacggctac gccggcgtcg atttccagga tcccgacgtg
    12781 accctcgacg ccctggaaca cgccgccgag cagctcgccc gcgccggctg cccccacttc
    12841 ctgctcacga tcatcaccgg ccccgacgac ttcctccgcg atcacttcgc ccggctcgcc
    12901 ggcttcctcg agcgcagcga acgcctgcgc cgccacatcc ccggcttcca tctcgaaggc
    12961 ccgttcctca acgacaatcc gggctaccgc ggcgcccacc gccccgaact gatgaccgac
    13021 ccgcagtggg accggttcgc ggcgtggcag gcggcctccg gcgaccgcat ccgcatgctg
    13081 acgatcgccc ccgaacgcgc cggcgccgtc gatttcatcc gccgcgccgt cgccgccggc
    13141 gtcttcctct gcgccggcca ttccgccgcc acccgccgcg acctgctggc cgccgtccaa
    13201 gccggtctgc gcctctacac gcacctgggc aacggcgccc cgctcgaaat cccgcgccac
    13261 gccaatgtca tccaggatgt cctctccgtc cccggcctgg ccgcctcgct gatcgccgac
    13321 ggcatccacc tcccgccata cgtcctggcc aatttcgccc gcgccctcgg ctcggcgcgc
    13381 ctcgtcctca ccaccgacgc gatggccgcc gccggcgccc cgacgggccg ctacgcactc
    13441 gccggcatcg cgctggaggt cggcgacgat cgcgtcgtgc gcaaccccaa tgcccccggc
    13501 ttcgccggct ccgccctctc gccgctcgaa ggcttctaca atggcatcca gttcgccggc
    13561 ctcggcgccg ccgcctcgtg gcacgcctgg acctggctcc gccgcgaact cttccccgat
    13621 ctcgcaccgc cgcgcatcgc tctccccttc cccgcccccc cctcctcccg ccaccggctg
    13681 ggcctctagt ccctccttcc tcggcttcgc ccttaccccc atcgccccgc cccagaattc
    13741 ggctctcggc tctcgtcctc gtcctcgtaa taatcccccc ccatccgccc cccgctctct
    13801 gaatcctttt gaggcatccc gcgctttctg ctacaacgtg gcggtatgca tgccgcgggc
    13861 gtcaatcgac cggctggctc ggccgcgccg gtgaccccgt ggcttcgacc cggttcccaa
    13921 aggatatccg tcatgcttgc gccgcttgaa tcgcaacgcc ggtcctgtta ctgcggcgat
    13981 gtgcgcaccg gccacatcgg ccagcagatc atcctcaagg gctgggtcca gaatcaccgc
    14041 gaccacggcg gcgtctactt cgtcgatctg cgcgaccgct ccggcctggt ccaggtcgtc
    14101 ttcgatccgg aggaactccc cgccgaagcc ttcgagcgcg ccacccgcct ccattacgag
    14161 gatgtcctcg ccgtccgcgg caccgtccgc catcgccccg agggcaccgt caacccgcgc
    14221 ctgaaaaccg gcgaggtcga ggtcctcctc tccgactacg ccatcctctc cgaatccgcg
    14281 cccctcccgt tcccgattga tgaattcacc cagtcggccg aggacctgcg cctgcgctat
    14341 cgctacctcg acctgcgccg cccgcgcatg cagcagacgc tcatcgagcg cgcccgcgtc
    14401 aacggcatca tccgccgcgc cctcgacgcc gagggcttca tcgaaatcga aacccccgtc
    14461 ctgacccgct ccacccccga gggcgcccgc gatttcctcg tccccagccg cctcaacccc
    14521 ggcacgttct acgccctccc ccagtccccc cagctcttca agcagctcct gatggtcgcc
    14581 ggcatggacc gctattacca gatcgtccgc tgcttccgcg acgaggacct gcgcgccaat
    14641 cgtcagcccg aattcaccca gctcgatatc gaaatggcct tcgtcacccc cgatgagctg
    14701 ttcgaaatca tggagcgcat gctgacggcc gtcttccgcg acgtgaaggg cattgacctg
    14761 aaaaccccct tcgaccgcat gacctacgcc gaggcgatga accgctacgg cagcgacaag
    14821 cccgaccgcc gcttcaagct cgaactgcac gacatcaccg cggccgtccg cgccggcggc
    14881 tgcgagttca aggtcttcgt cgatctgctc ggccaggggg gcgtcctgcg cgcgctcgtc
    14941 gtccccggcg gcggcgagaa gttctccaac acgcagctca agccgggcgg cgagctcccc
    15001 accttcgccg aacgccacgg cgcccggggc ctggcctggt tccgcaccgt ccaggacgac
    15061 agcgccgtcc gcctcgagtc ctccatctcc aagttcttcg acgccgcctg ccaggcccgc
    15121 atcatcgagg ccaccggcgc cggcgccggc gatctgatcc tcatcgtcgc cgaccagccc
    15181 atcgcctcgg cccaggccct cggccagttg cgcctcaagc tcggccgcga cctcggtctg
    15241 atccccaacg acgagttcaa tttctgctgg atcaccgatt tcccgctgct ggagtggaac
    15301 gccgacgaaa aacgctggtt ctccatgcac cacccgttca cgatgcccca ccccgaggac
    15361 tgggacaaac tcgaaagcga ccccggcgcg gtccggtcgc tcgcctacga cctcgcgctc
    15421 aacggcgagg agctcggcgg cggctcgatc cgtatccatc gccgcgacct gcaggaacgc
    15481 gccttccgcg ccctcggcat cggcgaggac gaagcccgga tgaaattcgg cttcctaatg
    15541 gatgccttcc agtacggcgc cccgccccac ggcggcatcg ccttcggcat ggaccgcctg
    15601 atgatgctcc tgctcggcct cgattcgatc cgcgacgtca tcgccttccc caaaacccag
    15661 accggcgcct gcctgatgac cagcgcgccg gccgaggtcg acgaggccca gctccgcgag
    15721 ctctccatcc gctccaccgc ccgaaaggag aagtagcagg tctccacggt ggcgccttga
    15781 ccgcgcgtgc ctacccctgc tcatctgtgc tcgacgatgg ttcctcgctg gcccggttcc
    15841 gcctctcccc tatgtctctt gtgtccctca tgtcccttca aggatgattc atcacggacc
    15901 agcttccaat gccaacgcta gagtttcagc cttatcagct ggtacagtcc gcgaggcgga
    15961 cgaccggaat agagcgcagg gtgacggccg caggccggaa ccctgcgtcg ccgcccccga
    16021 caatccaagc ccctgaacgg ggcggccgga accgatctga atttggggaa tgacaggcat
    16081 ttcagtggcg ggaaccggtt ccggcattca gaatcagaac ctttccgcag aggcgcatcc
    16141 aggatggctc aactctcccg cgaccgctcc ttcctgatgg tcattgacct ccaggaacgt
    16201 ctccttccgg cgatccacga ccatcaggcc gtccgcgacc gctccgtctt cctgatccgc
    16261 gccgcccgca tcctcggcct cccgatcgtc tggaccgagc agtatcgcaa gggcctcggt
    16321 cccaccgtcc cgccggtcgc cgaggccatc ggcgacgccg cccgcccgat ggaaaagatg
    16381 gccttcgggt gcctgtgcga cgacgccgtc gcccgcgcgg tcgagtccgc cggccgcgat
    16441 cagctcatcc tctgcggcat cgaggcccac gtctgcgtcc tgcaaaccgc cctggccgcc
    16501 cgcgaacgcg ggctgaccgt cttccttgcc gaggacgcca tcggcagccg ccgcccgtcg
    16561 gaccgcgacg ccgcgctgcg ccgcatggcc caggccggct gcgtccccgc caccagcgag
    16621 atgctcgtca tggaagccct gcgcgtcgcc ggcaccgacc cgttcaaaca gctcctgccg
    16681 ctgctcaagg gctgaggagg atcattaacg ctctcttgaa cctgtcgagt cgaatttgtt
    16741 tcgattttcg aatttcggat ttcgaatttt ccattcacgc tgatatggat tctctcccat
    16801 gatcgacatc gtcgccttcg gcgcccacgc cgacgacgcc gagcttttcg ccgccggcac
    16861 gctcgccctc gccgcccgcc gcggccgctc caccgccatc gtcgacctca cccgcggcga
    16921 aatggccacc cgcggcaccc ccgaaacgcg ccggcgcgaa gcccaggccg ccgccggcat
    16981 cctcgccctc acccaccgcg aaacgctcga cctcggcgac ggccgcctca ccaacgacca
    17041 cgcttcccgc gtcttggtcg cctccgtcat ccgccgcctc cgccccacgc tcgtcctcac
    17101 ccacgggcgc gaggaccgcc atcccgacca ccgcgccgcc cacgacctgg tccgcgacgc
    17161 cgccttcttc gcttacgtcg gcgaattccc cgccgacggc gaacgctggc gccccgccgg
    17221 cctcgccttc tatctcggca actcgttcca acccgaggcc cgcgccgatt gggtcgtcga
    17281 tatttcggca acctatgaaa tcaagatgca ggccctgcgc gcctatgcct cgcagttcct
    17341 tgccgacccc gcgcaacccg accgcgccac ttatatcgcc tcgcccgaat tctgggacgc
    17401 catcgaacgc cgctcgcgcc tctgggggca ccgcatcggc gtcgcccacg gcgagccgtt
    17461 cctcctcgat cgccccgcca gcgccctcca cccgctggtc caactcctcc cccgcccctg
    17521 aatttccgcc gcgtctgaaa tgcctccgcc cgaagcgcac cgcctctgca tcccgcagcg
    17581 attgcccggt gtcgttaaag cctgtgtggg accggcgccc gcgcggtcac ccgccccgca
    17641 tcccgcgggg atgccggacc gtagccggtg gttgagtgca gcaataccac cggtcgcctc
    17701 ggtcacccct tcctccgcat cccgcgggga tgccggacgc ccgccccacc agtccgcgga
    17761 gccgacgacc ggaataaagc acagggtgac ggccgcgggc cgaaaccctg tgccgccccc
    17821 ccccaacgcc caaacccttg caaggggggc ccggaaatcc gccccccgg
//
LOCUS       DAOPCO010000050        17781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3264_length_17781_cov_5.358908, whole genome shotgun sequence.
ACCESSION   DAOPCO010000050 DAOPCO010000000
VERSION     DAOPCO010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 17781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17781
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3264_length_17781_cov_5.358908"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLS90_05680"
     CDS             <1..580
                     /locus_tag="PLS90_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01928.1"
                     /translation="DEFWERWPWPSGQAPVNLLERDPTAMDMLWRGRVTTSTVTLPSF
                     VDSFLLDERVSVLGVGGLERQELGYFNRENKLYPIVDGVEFSQNIAASLDFVSDRPVR
                     RIFLAYEATPALGVYPILLLSINNDPYVPVYLDSPEPDLVAVDVDRPAGSYRLTLLYL
                     NDASFVWSERNIHEDRNFKLYRIALVHVAPPE"
     gene            590..1771
                     /locus_tag="PLS90_05685"
     CDS             590..1771
                     /locus_tag="PLS90_05685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK01929.1"
                     /translation="MAGKPTVLYLLDYTDLGGGETSFLALLEEMARREWPVRPVVILN
                     RPGPVAERLAALGIAAEIVGYPLRLRRGPLPWFSPAAAARIGRIARERDATLLHAIHF
                     FGMVYGGFAARRLGLPLVWTCHGWFDVDRPVKRILARRFAAHIACVSDAVREEASRRI
                     GPGRPVTTNYLGVAPYPDAGPQPARAAVRAELGVDDATPVVGMIGRFQPIKGHDLLLD
                     ALRRVLPRRPGLKVWIIGDALFGSVEEAAHRRRIEARVEAEGLGPVVNFLGFRRDARR
                     VMRALDLLVIPSARESFSMVAVEGLEAGIPVVGPDGWGPREIIESPATGLRFRPGDAE
                     DLARCIGALLERTGDGAAFSPAAGPDRVRRLFSVAAHLERTLAIYRELTGERTGGEKE
                     N"
     gene            1978..3384
                     /gene="purF"
                     /locus_tag="PLS90_05690"
     CDS             1978..3384
                     /gene="purF"
                     /locus_tag="PLS90_05690"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPK01930.1"
                     /translation="MHREMPHEECGIVGVYGHPEAAKLVYLGLYALQHRGQESAGIVS
                     SDGENLHAYKDKGLVNDVFDQHRLNELTGSTAIGHVRYSTTGSNRVVNAQPLTANFRG
                     GTMAVAHNGNIVNVTELRAELETRGAIFMGTADSEVVLHLIAQSGTPDFIEALVTALY
                     RLKGAYSLLMLREHQLIAIKDPRGFRPLCIGSLDGAIVIASESCALDIMGAQLLREIR
                     PGEMVIVEDEGVRIESPFPPVEEKFCVFEYIYYARPDSVINGRSIYEIRHRLGEQLAH
                     EQPAEADVVIAVPDSSNPAAIGYAKALGLPYDKGLIRSHYVGRTFIEPDEGIRHFGAK
                     LKYNPVRSVLEGKRVVVVDDSIVRGTTSIKIMEMIRRAGAREVHLRLTAPPWKHPCYY
                     GIDTPSESELMANQLSVEEMARKIGCDTLGFISVEGLLQVVPRTQGYCTACFSGEYTA
                     GKPTDFRKNRLEAHAVLE"
     gene            3458..6244
                     /locus_tag="PLS90_05695"
     CDS             3458..6244
                     /locus_tag="PLS90_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK01931.1"
                     /translation="MRFRFFFATLDAGKSKESDPMLALESFVRERGLPAMLSTALGRR
                     GLSDWTPLQARAWRAGADRPDSDWLVTAPTSAGKSLVAELRSARHLARSEKVVWLAPT
                     RALADATARQLAADFAPCGWRVALATGDAPDGDAALARGSFDLLVTVPEKALAWLARE
                     PHALAGVGLVVVDELPLLGDPERGPRFDLLLALIRHSPYAPRRLALAPPLGEGRAAAA
                     WWGGRWLDEPSRPRPLHEGVLDVRTGRFRWRDRAAGEAGELPLIPPEVLLECLSRAAA
                     TVGIDDHPPLRALTGALAGTLAARGEPTLLFAPTRAMARTWALGLAGALEPTLDPELL
                     GRLNRLARLEPCSDLAAAAAAAAGGVGLHHADLPAPARQAVEEAFTDGKLNLVVATPT
                     LAHGVNLRARNVVLWPHAPGGGPRRAAWLPLERYHDQAGRAGRMGWGEGPGRSILVAW
                     DGAAAERLWDRFIAPPPPPLRSALALAGLAPLLPPALAGDVARTRPNLLALMASTLGG
                     TQRPADELGGLEWALDQALADCDAAHLLRPRADAAEPHRLSSRGQIAALHGLAPGQAR
                     HLGRVAEESPPGEDPLPCLWGLALAAPPATWPGARPGRSTISAEARAWFRQRDLRPPG
                     WLAERLDRPGGPEPEDLAALEAGARLARWIGPEPTVEIEHATGWSAGMLQRAGEALAA
                     LAAAAAELAALRGLPATDGARWRELAARLRRGLPAGAAELGRLDLPDLGRTGLLRLWR
                     DGLASVDALRDAPEQLLAERLEDARLARRVRLRVAAIESPCGLTAMGGEETARGPAGA
                     PAELDAHREPTPEPSRSGPAGVGCPTLAIDLTSPCLVRVGEREVRLNPLGFDLLVMLA
                     EEPGRVVQRDALYHRLWGEDGPEDQQLDRHRRLVEAALRPALNGDGPRPVIEVVRGVG
                     FRLALPAAAVRVRT"
     gene            6511..8607
                     /gene="ppk1"
                     /locus_tag="PLS90_05700"
     CDS             6511..8607
                     /gene="ppk1"
                     /locus_tag="PLS90_05700"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003918.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPK01932.1"
                     /translation="MSLVDSRTVISEVDPRDPAQRYLNRESSWLAFNRRVLDEALAPH
                     NPLLERVNFLAISANNLDEFFMKRVGGLQRQLAAGVTKLSMDGKRPLEQLQMIRAIVL
                     QEAAEQADLLVETLIPELHEQGVHIHGWAELTAAQRDWVTDYFDKMILPILTPLGVGP
                     GQPFPFISNLSLSLAVLVRRHGERQPRFARVKVPANRPRFIQVPDELTFVRLEEIIAH
                     NIGRLFVGMEIIEATPFRVTRNADLERNEEEADDLLEMIEEELRNRRFAPIVRLETVD
                     TISEELLAWLIQELGVDSARDVYQSRAPLGLADLVEIARLDVPALRFNTPAPVLHPRL
                     RPQDSEHDAGVDLFSVIRQGDLLVHHPFQSFATSVLALLQAAAVDPHVVAIKQTLYRT
                     APNSPIVAALLKATEEGKEVNVQVEIKARFDEANNIEWVRRLESAGAHVTYGFIGLKT
                     HSKTMLIVRDEPEGTRRYVHIGTGNYHTGTARLYTDLGLFTCDPALCQDVADLFNYLT
                     GHSAFSDYRKLLVAPVNMRRRFLEMIRRELEHAAAGRPGRIIAKMNQLEDQEIIDALY
                     EASQGGVQIDLIVRGFCCLRPGVPGLSENIRVMTIIGRFLEHSRIYYFGNDGRPEYYI
                     GSADWMSRNLDWRVEAVTPIESPALQKELDFILRTVMNDHAQAWDLHADGTYVRRRPR
                     RGKGSQAQFLKHYHKL"
     gene            complement(8678..9931)
                     /locus_tag="PLS90_05705"
     CDS             complement(8678..9931)
                     /locus_tag="PLS90_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01933.1"
                     /translation="MLAALFHPRWIEPRLLDALADSPVVLVHGPRQCGKTTLARHVGE
                     RQGYIYYTFDDGALRASAETDPVGFIDALPAKVVLDEVQRVPELFTAIKARVDRERRP
                     GQFLLTGSTNVLLLPQLSDSLAGRIEILRLHPLARAEIAATPSSLFESLFSRRLPAVR
                     GSRLGSELPELLTAGGFPAARARSAASRRAVWYRDYVETLVQRDVQDLARIHSVEALP
                     RLLILAAGQTARLFNVSALAAPFQLSRPTIRDYITLLERLFLLELLPAWHSNRISRLV
                     KTPKLHVTDTGLACALLGVDAEQLKQDRGLLGQILETFVCQELRRIASWEDTPPRFYH
                     FRNKDRVEVDVVLEGRAGRIVGVEVKAAATVKEEDFAGLRKLERAAGDRFVYGVVFYD
                     GEQSLPFGEGLAAVPIRALWEAPAG"
     assembly_gap    9933..10032
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10199..11953)
                     /locus_tag="PLS90_05710"
     CDS             complement(10199..11953)
                     /locus_tag="PLS90_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPK01934.1"
                     /translation="MVGPFSRLSKEHIVAEPGFNRWKVPPASIAIHLCIGSVYAWSIF
                     NPPLTRIHGVVTSASTDWALGQVVWVFTVAIVFLGLSAAVGGKWLEKVGPRMVGVTAA
                     CCWGGGFLLGSLGIVTHQLWLLYVGYGAIGGCGLGLGYVSPVSTLIRWFPDRRGMATG
                     MAIMGFGGGAMIGAPLKEFLLGLYSRAPQSLGALADVTLTTQGGVRYASLGGRLREVV
                     VDADTVYLVGTGSTGVAATFLTLGLLYFAVMLLAAFAYRVPAPGWKPAGWNPEANGTA
                     RKMITNNHVHIDRALFTPQFYLLWIVLCFNVTAGIGVLGVAKTMMTEIFGNRLPTIVD
                     DAFAATFVMLISIFNMTGRFFWSSASDYLGRKNTYFIFFILGIVLYLSIPYCARQVSA
                     DPKVVWLVYFYAASMIIFTMYGGGFSTIPAYLADIFGTRYVGGIHGRLLTAWSTAGVL
                     GPLAITSLRDRARLGAIHELAAQVDPAAFAAKFGAPLSQLELLVEKNTVTIANLLEIA
                     PAGTVDPSSTLYNSTMYLMAGLLAVALVANALVRPVHEKHHMEEEEAAAPAPASCGPG
                     RASSPAPAPSLARQPVSR"
     gene            12253..13272
                     /locus_tag="PLS90_05715"
     CDS             12253..13272
                     /locus_tag="PLS90_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01935.1"
                     /translation="MMLLDWTGSRSIGRFVLLGALAWVHAAAAGESPTIAGQPIFPAD
                     HIWNTPVDQLPLDSQSAVYVATIGAEATVHPDFGSGLYNGGPIGIPYLVVEAGQPTVP
                     VSFEYDDESDPGPYPIPSDAPIEGGHAADGDRHVLVLDRGSGLLYELFAAFPQGGGES
                     WSAGSGAIFDLNGYALRPAGWTSADAAGLPILPGLVRYDEVAAGEIRHALRFTAPQTR
                     RAWVWPARHFASDLTGPEYPPMGQRFRLRWDFDESGFAPECQVILRALKRYGMILADN
                     GSAWFISGAPDERWDNTALRQLRQIPGREFEAVDCSGLMLAEDSAQALQPQTGLSADR
                     MDRYR"
     gene            complement(13321..14421)
                     /locus_tag="PLS90_05720"
     CDS             complement(13321..14421)
                     /locus_tag="PLS90_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01936.1"
                     /translation="MESPAADPAALLTLAWTGPAAAMAVLLILVQTLRWVALGAAGLA
                     ALLALGQALDRPPADEREAVVARQRASRLRRIPPVALTAALAAELLLFGVRWGLMRAG
                     AGGAGLVWIALPWPEGTPPLALALAALTALTWGAALAGRRIGKLLPLFVHGLVLAGIA
                     FAFSAEPLSTSGAGLPGPELWGRFFHRLLAGLVAGGLALAVGEAFGAPPSDSPAGAAR
                     RDLAKLGALLATLGIFLQVATGLWLMLVEPPVAQSTLLRPGGFGLVWMTSIGAVMFLF
                     MLAAATLALPRRRLAAGGAGLMLALVLAGMLAGRLGTRAALLAAGGGPGRLDAPLAAP
                     AHYPWITFAALGLALGLTRLAQRMVFSAPGRG"
     gene            14658..15629
                     /locus_tag="PLS90_05725"
     CDS             14658..15629
                     /locus_tag="PLS90_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012913575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPK01937.1"
                     /translation="MPLRSFFPLLLLGLALACACGSPDTPDNPASGAEPSPQTATFDG
                     EYPIRVVTTTGMIADLAREIGGERVRVEALMGPGVDPHLYKASTDDVSRLRGADLILY
                     NGLHLEGKMADVLERLSKRQPTFAVADQLPAEKLRTPPEFQGLHDPHVWFDVALWARV
                     GGRVADLLAEFDPANAAEYAARAKEYLDRLNALDAWVRDRIATIPEPQRVMITAHDAF
                     GYFGAAYDVEVMGIQGLSTESEAGVRRVNELVDLLVAREVKAVFIESSVSDKNIRALV
                     EGSAARGHAVRIGGQLYSDAMGPAGSGADHYEGMIRHNVETIVGALR"
     gene            15713..16501
                     /locus_tag="PLS90_05730"
     CDS             15713..16501
                     /locus_tag="PLS90_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPK01938.1"
                     /translation="MKATADKATGAEPPLRVRDLTVAYHRKPVLWDVELTAPEGKLIG
                     IIGPNGAGKSTLIKAVLGLVPRASGEVLVYGKPYEANRRLVGYVPQRGSVDWDFPVDA
                     LDVVLMGRYGRLGWIRRPGRADRQAAMNCLEKVGMAAYAHRQISQLSGGQQQRVFLAR
                     ALAQEAQLYFMDEPFAGVDAATEKAIVALLHELKASGRTCLIVHHDLQTVSEYFDHAI
                     LLNMRVVAQGPTAEVVTRDNLHKTYGGRLTLLEEAATEMARARR"
     gene            16587..>17781
                     /locus_tag="PLS90_05735"
     CDS             16587..>17781
                     /locus_tag="PLS90_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK01939.1"
                     /translation="MNWLAYNTLIVLAGTSLLGIVAGVLGSYMVLRRRALLGDALSHA
                     AYPGLGLAFLITGQRHFGGLLTGAFATALIGSWTVGAIRRHTRVKEDAAIGIVLSVFF
                     GLGVVVSRIVQNTTTTGSKAGLDSFIFGKSSGMVAADVRAILLVGVVVLAATLLLAKE
                     FRLLAFDPDFGRVQGWPILLLDIIMMSLVCVTTIVGLPAVGVVLMAALLIIPGAAARF
                     WTDRLGRMLWLAGLFGGVAGACGTLASAWAPSNVGAGPVIILTAAGCFLFSMLAAPRR
                     GAVARVLRHVALARRVAMQNLLRTLYELSEPAWPDPAPLRPGDLTAARSWSAAQARRL
                     LRRAAARGWVEPNGDGWRLTGAGWRRAAEVTRAHRLWEIFLIEEAQVAPTLVDQLSQR
                     GTECSG"
BASE COUNT         2798 a         5625 c         6616 g         2642 t
ORIGIN      
        1 ggatgagttc tgggagcgct ggccctggcc gtcgggtcag gcgccggtca acctgctgga
       61 gcgcgacccg accgcgatgg acatgctctg gcgcggccgg gtcaccacct ccacggtgac
      121 gctgcccagt ttcgtcgaca gctttctgct cgatgagcgc gtcagcgtgc tgggcgtggg
      181 gggactggaa cggcaggaac tggggtattt caaccgggag aacaagctct atccgatcgt
      241 cgatggggtg gagttcagtc agaacatcgc cgcgtcgctg gatttcgtgt cggatcggcc
      301 ggtccggcgc atctttctgg cgtatgaggc gacgcccgcc ctgggggtct atccgatcct
      361 gctgctgagc atcaacaacg atccgtatgt gccggtgtat ctggattcgc cggagccgga
      421 cctggttgcg gtcgacgtcg accgaccggc cggcagctac cggttgacgc tgctgtatct
      481 caatgacgcc agttttgtct ggtccgaacg caacatccac gaagaccgca acttcaagtt
      541 gtaccgcatc gcgctggtcc acgtcgcgcc gcccgaataa gagccgccga tggccgggaa
      601 gcccaccgtc ctctaccttc tcgattacac ggatctgggc ggaggggaga cgagttttct
      661 ggcgttgctg gaggaaatgg cgcggcgcga gtggcccgtc cgcccggtcg tcatactgaa
      721 ccggccgggg ccggtggccg aacgtctggc cgcgctcggc atcgccgccg agatcgtcgg
      781 ctatccgctg cgtctgcggc gcgggccgtt gccgtggttc agcccggcgg cggcggcgcg
      841 catcgggcgg atcgcgcgcg aacgcgacgc gacgctgctg cacgcgatcc acttcttcgg
      901 catggtttac ggcggattcg ccgcgcggcg gcttggcttg ccgctcgtct ggacgtgcca
      961 tggctggttc gatgtggacc ggccggtcaa gcggatcctg gcgcgccgtt tcgccgcgca
     1021 catcgcctgc gtttcggatg ccgtgcgcga agaagccagc cgacgcatcg gtcccggccg
     1081 gccggtcacg acgaactacc tgggcgtggc gccgtatccg gatgcggggc cgcaaccggc
     1141 gcgagcggcc gtccgcgccg agctgggggt ggacgacgcg acgccggtcg tggggatgat
     1201 cggccggttc cagccgatca agggccacga cctgctgctc gacgcattgc ggcgggtgct
     1261 gccgcggcgg cccgggttga aagtctggat catcggcgac gcgctgttcg gcagcgtcga
     1321 ggaggcggcc caccggcgtc ggatcgaagc gcgggttgag gcggaaggcc tcggcccggt
     1381 ggtgaatttt ctcggctttc gtcgggacgc gcggcgagtg atgcgcgcgc tggatctgct
     1441 cgtcatcccg tcggcgcgcg agtcgttttc gatggtcgcg gtcgaaggat tggaggccgg
     1501 cattccggtg gtggggcccg atggctgggg cccgcgcgag atcattgagt cgccggcgac
     1561 gggcctgcgc ttccggcccg gcgacgcgga ggatctcgcc cgctgcatcg gcgcgctgct
     1621 cgagcgaacg ggtgacggag cggcgttttc gcccgccgcc gggccggatc gcgtccggcg
     1681 gctgttcagc gtcgcggcgc acctggagcg gacgctggcg atttaccggg agctgacggg
     1741 cgagcgaacc ggcggcgaga aggaaaattg atcacgaaag ggcgcgaaag gcacgaaaga
     1801 attaatttgt aggtcctttc cccggcggca gcaaaaaagc catctagtca gcccgtcggg
     1861 gctgggcata taataggatc gatggcgggc gcggattcac cgcccgatgg atgggtcccc
     1921 gccgccgccg accggcgatt cacccccaca ctcggcctcc cgacggaagg accacgcatg
     1981 catcgcgaaa tgccccatga ggaatgtggg atcgtgggtg tctacggcca ccccgaagcg
     2041 gccaaactcg tctatctggg cctgtatgcc cttcaacacc ggggccagga aagcgccggg
     2101 atcgtctcga gcgacggcga gaatctgcat gcctacaagg acaaggggct ggtcaacgac
     2161 gtcttcgacc agcaccgcct gaacgagttg accggctcga cggcgatcgg ccacgtgcgc
     2221 tattcaacga ccggatccaa ccgggtggtc aacgcgcagc cgctgacggc caatttccgc
     2281 ggcggcacga tggcggtggc gcataacggc aacatcgtca atgtgaccga gctgcgggcc
     2341 gagctggaga cgcgcggcgc gatcttcatg gggacggccg attcggaggt cgtcctgcat
     2401 ctgatcgcgc aatcgggcac gcccgatttc atcgaggcgc tggtcaccgc gctctatcgg
     2461 ctcaaggggg cgtattcgct gctgatgctg cgcgagcatc agctcatcgc gatcaaggat
     2521 ccgcgcggtt tccgcccgct gtgcatcggt tcgcttgacg gggcgatcgt gattgcctcc
     2581 gaatcgtgcg cgctggacat catgggggcg cagctgctgc gcgagatccg gccgggcgag
     2641 atggtcatcg tcgaggatga aggggtgcgg atcgagtcgc cgttcccgcc ggtcgaggaa
     2701 aaattctgcg tgttcgaata tatctactat gcgcggcccg actcggtgat caacgggcgt
     2761 tcgatctatg aaatccggca tcggctgggc gaacagctcg cccacgagca accggccgaa
     2821 gccgacgtcg tcatcgccgt gcccgattcc tccaatcccg cggcgatcgg gtatgccaag
     2881 gcgctggggc tcccctacga caaggggctg atccgctcgc attacgtggg gcggacgttc
     2941 atcgagcccg atgaggggat ccggcacttc ggcgcgaagt tgaaatacaa cccggtccgc
     3001 tcggtgctgg agggcaagcg cgtcgtcgtc gtcgacgact caatcgtgcg cggcacgacc
     3061 agcatcaaga tcatggagat gatccgacga gccggcgccc gggaagtcca cctgcgcctg
     3121 accgccccgc catggaagca tccgtgttac tacggcatcg acacgccgag cgagagcgaa
     3181 ctgatggcga accagttgag cgtcgaggag atggcgcgca agatcggctg cgacacgctg
     3241 gggttcatct cggtggaggg gctgctccag gtggtgccgc gaacccaggg gtactgcacc
     3301 gcgtgtttca gcggggaata caccgccggc aagccgaccg atttccgtaa gaaccggctg
     3361 gaagcgcacg cggtgcttga atgaggcaga cggcgcgggg ggaattccaa ggacaccatt
     3421 atagggggaa tgccgggggc tcgggcgttg tttcggcttg cgttttcgct ttttcttcgc
     3481 cacgctcgat gccggcaagt cgaaggagtc cgaccccatg ctggcgctgg agtcatttgt
     3541 tcgcgaacgc gggctgccgg cgatgctgtc cacggcgctt ggccgccgcg ggctgagcga
     3601 ctggacgccc ctgcaggcgc gcgcgtggcg ggcgggcgcc gaccggcccg acagcgactg
     3661 gctcgtgacg gcgccgacgt cggccggcaa aagcctcgtc gccgagctgc gctcggcccg
     3721 tcatctggcc cggtcggaga aggtcgtctg gctggcgccg acgcgcgcgc tggccgatgc
     3781 gaccgcccgg cagctcgccg ccgatttcgc cccgtgcggc tggcgcgtcg cgctggcgac
     3841 cggcgacgcc cccgacggcg acgccgcgct ggcccgcggc agcttcgatc tgctcgtgac
     3901 cgtgccggaa aaggcgctgg cctggctcgc gcgcgagccg cacgcgctgg cgggcgtcgg
     3961 gttggtcgtc gtcgacgagc tccccctgct gggcgatccg gagcgcggcc cgcggttcga
     4021 cctgctgctg gcgctgatcc ggcactcgcc gtatgcgccg cggcggctgg cgctggcgcc
     4081 gccgctcggc gaggggcggg cggcggccgc gtggtggggc ggccggtggc tggatgagcc
     4141 gtcgcgaccg cgaccgctgc acgagggggt gctcgacgtg cgcacgggcc gattccgctg
     4201 gcgcgaccgc gcggcgggcg aggcgggcga gctgccgctg atcccgcccg aggtcctgct
     4261 ggaatgtctg agccgggcgg cggccaccgt ggggatcgac gatcatcccc cgctgcgcgc
     4321 gctgaccggc gcgctggccg gcacgctggc cgcgcgcggc gagccgacgc tgctgttcgc
     4381 gcccacgcgc gcgatggccc gcacctgggc gctggggctg gccggggcgt tggaaccgac
     4441 gctcgatccc gagctgctgg ggcggctcaa ccggctcgcc cggctcgagc cgtgcagcga
     4501 ccttgccgcg gcggcggccg ccgcggccgg gggcgtcggc ctgcatcacg ccgatctgcc
     4561 cgccccggcg cgccaggccg tggaagaggc ctttaccgac ggaaagctga atcttgtcgt
     4621 cgcgacgccg acactggcgc atggagtcaa tctgcgcgcg cgcaacgtcg tgctgtggcc
     4681 gcatgcgccc ggcggcggtc cgcgccgcgc ggcctggctg ccgctggagc gctaccacga
     4741 ccaggcgggc cgggccgggc gcatgggatg gggcgaaggg ccggggcgct cgattctggt
     4801 ggcgtgggac ggcgccgccg ccgagcggct atgggaccgg ttcatcgcgc ccccgccgcc
     4861 gccgttgcgc tcggcgctgg cgctggccgg gctggcgccg ctgctgccgc cggcgctggc
     4921 gggcgatgtc gcgcgaacgc gcccgaacct gctggcgttg atggcgtcga cgctcggcgg
     4981 cacgcaacgc ccggccgatg aactgggcgg gctggagtgg gcgttggacc aggcgctggc
     5041 cgactgcgac gcggcacacc tgctgcgccc gcgcgccgac gcggccgaac cccatcgcct
     5101 gagcagccgg ggtcagatcg ccgcgctgca tgggctcgcg ccgggccagg cgcgtcacct
     5161 cggtcgcgtc gcggaggaat cgccgcccgg cgaggatccg ctgccgtgcc tgtggggcct
     5221 ggcgctggcg gcgccgccgg cgacgtggcc gggcgcacgg ccggggcgtt cgacgatcag
     5281 cgccgaggcg cgcgcctggt tccgccagcg cgatctgcgg ccgccgggct ggctcgccga
     5341 gcggctcgac cgccccggcg gacccgagcc ggaggatctg gcggcgctgg aggccggcgc
     5401 ccgcctggcc cgctggatcg gaccggaacc gaccgtcgag attgagcacg cgaccgggtg
     5461 gagcgcgggg atgctgcaac gcgccggcga ggcgctggcc gcgctggcgg cggcggcggc
     5521 tgagttggcc gcgctgcgcg gactgccggc gaccgacggg gcgcgctggc gcgaactggc
     5581 cgcccggctg cggcgggggc tgccggccgg cgccgccgag ctcggccggc tcgacctgcc
     5641 tgatctgggg cgcacgggct tgctgcggct gtggcgcgac ggcctggcct ccgtggacgc
     5701 gctgcgcgac gcgccggagc aactcctcgc cgagcggctg gaggacgcgc ggcttgcgcg
     5761 gcgggtccgg ctgcgcgtgg cggccattga gtcgccgtgc ggcttaacgg ctatgggtgg
     5821 cgaggaaacc gcgcgcgggc cggccggggc gccggccgag ctggacgcgc atcgcgaacc
     5881 gacgccggaa ccgtcgcgca gcgggccggc cggggtgggg tgccccacgc tggcgatcga
     5941 cctgacgtcg ccctgcctgg tgcgggtcgg cgagcgggaa gtgcggctca atcccttggg
     6001 attcgatttg ctggtcatgc tggccgaaga gccggggcgg gtcgtgcagc gcgacgcgct
     6061 ctaccatcgg ctgtggggcg aggacgggcc cgaggaccag caactggacc gccaccggcg
     6121 tctggtggaa gcggcgctgc ggccggcgct caatggcgac gggccccggc cggtcatcga
     6181 ggtcgtgcgc ggcgtcgggt tccggctggc gctgccggcg gcggcggtcc gggtgaggac
     6241 gtgagggaga gcgggatcag ggacataacg gacacaaagg acgtaaggga catcagagaa
     6301 agcagctgat tcaaggcggg atttcgagcg ccgaggacga gggacgagag acgaattttg
     6361 ggcgggcctt tgggggcgat gacaacgacg acgacgaggg cgaggggcga agaggaggga
     6421 cggaacccgg cgggtggcgg gggcgtgaca gagggtactt ggatcggcta caatacggcc
     6481 tctccgcagc gtaggaggcg cgcgcccgcc gtgagtctgg tcgattcccg caccgtcatt
     6541 tccgaagtcg atccgcgcga tccggcccag cgttacctca atcgcgagag ttcgtggctg
     6601 gcgttcaatc gccgggtgct ggacgaggcg ctggcgccgc acaacccgct gctggagcgg
     6661 gtcaattttc tggcgatctc ggccaacaac ctggatgagt ttttcatgaa acgcgtcggc
     6721 ggcctgcagc gccagttggc cgccggcgtg accaagctgt cgatggacgg caagcgcccg
     6781 ctcgaacagt tgcagatgat ccgcgcgatc gtgctgcagg aggccgccga gcaggccgac
     6841 ctgctggttg agacgctgat ccccgagctg catgagcagg gcgtgcatat ccacggctgg
     6901 gcggaactga ccgccgccca gcgcgactgg gtgaccgact acttcgacaa gatgatcctg
     6961 ccgatcctga cgccgctggg ggtcgggccg ggtcagccgt ttccgttcat ctcgaatctg
     7021 agcctgtcgc tggcggtgct ggtccgccgc cacggggaac gccagccgcg cttcgcgcgc
     7081 gtcaaggtgc cggccaaccg gccgcggttc atccaggtgc ccgacgagct gacgttcgtg
     7141 cggctggagg agatcatcgc gcacaatatc ggccggctgt tcgtcggcat ggagatcatt
     7201 gaggccacgc cgttccgcgt gacgcgcaat gccgacctgg agcgcaacga ggaagaggcc
     7261 gacgacctgc tggagatgat cgaggaggag ctgcgcaacc ggcggttcgc cccgattgtc
     7321 cggctggaga cggtcgatac gatcagcgag gaattgctgg cctggctgat ccaggagctg
     7381 ggcgtggaca gcgcgcgcga cgtttatcag tcgcgcgcgc cgctgggact ggccgatctg
     7441 gtggagattg cccggctcga tgttccggcg ctgcggttca atacgccggc ccccgtgctg
     7501 cacccgcggt tgcggccgca ggactcggag cacgacgccg gcgtcgacct gttcagcgtc
     7561 atccgccagg gcgacctgct ggtccaccat ccgttccagt cgttcgcgac gagcgtgctg
     7621 gcgctgctgc aggccgccgc cgtggacccg cacgtcgtcg cgatcaagca gacgctctac
     7681 cggacggcgc ccaacagccc gatcgtcgcg gcgttgctca aggcgaccga ggagggcaag
     7741 gaagtcaatg tccaggtgga gatcaaggcg cgcttcgacg aggcgaacaa catcgagtgg
     7801 gtccggcggc tggagagcgc cggcgcccac gtcacctacg gcttcatcgg gttgaaaacc
     7861 cacagcaaga cgatgctgat cgtgcgcgac gaaccggagg ggacccggcg ctacgtccac
     7921 atcggcacgg gcaactatca caccggcacg gcgcggctct acaccgatct ggggctgttc
     7981 acgtgcgacc cggcgctgtg ccaggacgtg gccgacctgt tcaactacct gacggggcac
     8041 tcggcgttca gcgattaccg caagctgctg gtggcgccgg tcaacatgcg gcggcgattc
     8101 ctggagatga tccgccgcga gctggagcac gccgccgccg gccggcccgg ccgcatcatc
     8161 gccaagatga accagctgga ggaccaggag atcatcgacg cgctctacga ggcgagccag
     8221 gggggcgtcc agatcgatct gatcgtgcgc gggttctgct gcctgcggcc gggcgtgccg
     8281 gggctgtcgg aaaacatccg cgtgatgacg atcatcggcc ggttcctgga acactcgcgg
     8341 atctactact tcggcaacga cgggcggccg gagtattaca tcgggtcggc cgactggatg
     8401 agccgcaacc tggactggcg ggtcgaggcc gtcacgccga ttgaatcgcc cgcgctgcag
     8461 aaggaactgg atttcattct gcggacggtg atgaacgacc acgcgcaggc ctgggatctg
     8521 cacgccgacg ggacgtacgt gcgccgccgc ccgcggcgcg gcaaaggctc ccaggcccag
     8581 ttcctgaagc attatcacaa gttgtaggcg cgcgggccag cgcgggcaaa cggcgggttg
     8641 agccgattcc gcccgccgcg atgaatcgag aatggaatca gccggccggc gcttcccaga
     8701 gcgcccggat tggcacggcg gccaggccct cgccgaaggg gaggctttgt tcgccatcgt
     8761 agaagaccac gccgtagaca aagcggtccc ccgcggctcg ttctaatttt cgcaggccgg
     8821 caaagtcttc ttccttcacc gtcgcggccg ctttgacctc gacccccacg atgcggccgg
     8881 cgcgaccttc cagcacaaca tcgacttcga cgcgatcctt gttgcggaaa tgatagaagc
     8941 gaggcggcgt gtcctcccag gaggcgatgc gccgcagttc ctggcagaca aaggtctcca
     9001 gtatctgccc cagaagcccc cgatcctgtt tcaactgctc ggcatccacg ccgagcaacg
     9061 cgcaggcgag acccgtatcg gtgacgtgca gcttcggggt cttgaccagg cggctgatcc
     9121 ggttgctgtg ccaggcgggc aacagctcca gcaggaacag gcgctccagc aaggtaatgt
     9181 aatcacggat ggtgggtcgg ctcagttgaa acggcgccgc cagagcgctc acgttgaaca
     9241 ggcgggccgt ttgcccagcc gccaggatca gcagacgcgg cagggcctca acggaatgga
     9301 ttcgcgccag atcctgaacg tcgcgctgaa ccagcgtctc gacgtaatcc cgataccaga
     9361 cagcacggcg gctcgccgcg gaccgcgccc gtgccgcggg gaaaccgccc gccgtgagca
     9421 gctcgggaag ctcggaaccc aggcgcgacc cccggacggc ggggagacgc cggctgaaga
     9481 gcgattcgaa gagggacgaa ggcgtagctg caatctcggc tcgggccaat ggatgcagcc
     9541 gcaggatttc gatgcggccg gcaagggaat cggaaagctg tggcaggagc aggacattgg
     9601 tcgagccggt caggagaaat tgcccgggcc gtcgctcccg gtccacacgg gctttgatgg
     9661 cggtgaacag ctcgggcacg cgctgaactt cgtcgagcac gactttggcg ggtagagcgt
     9721 cgatgaaacc cactgggtcg gtttcagcag aggcgcgcaa cgcgccgtca tcaaacgtgt
     9781 aatagatata gccctgccgc tcgcccacat gccgggcgag ggtggtcttc ccgcattggc
     9841 gcggaccatg caccagcacg acgggcgagt cggccaaggc atcgagcagg cggggctcga
     9901 tccagcgggg atggaaaagt gcggcgagca tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnggcatcga gcaggcgggg ctcgatccag cggggatgga aaagtgcggc
    10081 gagcatgacg gctgattgta atgttaagtc tcggccaatt gaaagaatca ggttcgccaa
    10141 ttgaaagtcg ggctgccctc gcggggcaac ccgactttta taaggcactg atttgaattt
    10201 atcgactcac tggttgccgg gccagactgg gcgcgggagc gggtgaggat gcgcgaccgg
    10261 ggccgcagct tgcgggggcg ggcgcggcgg cctcttcctc ctccatgtgg tgcttttcgt
    10321 gcaccgggcg gacgagggcg ttggcgacca gcgcgacggc cagcaggccc gccatcagat
    10381 acatcgtcga gttgtagagg gtgctcgaag ggtcgaccgt gccggccggc gcgatttcga
    10441 gcaggttggc gatcgtgacg gtgttcttct cgaccagcag ctcgagctga gacagcggcg
    10501 cgccgaactt ggcggcgaac gcggccggat cgacctgggc ggccagttca tggatggcgc
    10561 cgagacgggc gcggtcgcgc agcgaggtga tcgcgagcgg ccccagcacg ccggcggtgc
    10621 tccaggccgt cagcaggcgg ccatggatgc cgcccacgta gcgcgtcccg aaaatgtcgg
    10681 ccaggtatgc cgggatcgtg gagaaaccgc cgccgtacat cgtgaagata atcatgctgg
    10741 cggcgtagaa atagaccagc cagaccacct tgggatcggc gctgacctgc cgcgcgcagt
    10801 aggggatcga cagatagagc acgatgccca ggatgaagaa gatgaagtag gtgttcttgc
    10861 ggccgaggta atccgaagcg ctggaccaga agaagcggcc ggtcatgttg aagatgctga
    10921 tgagcatgac gaaggtggcg gcaaacgcgt cgtcgacgat cgtgggcaga cgattgccga
    10981 agatttcggt catcatcgtc ttggcgacgc ccagcacgcc gatgccggcg gtgacgttga
    11041 agcacagcac gatccacaac aagtagaact ggggggtgaa gagggcgcga tcgatgtgaa
    11101 cgtggttgtt ggtgatcatc ttgcgggcgg ttccgttggc ttccggattc cagccggccg
    11161 gcttccagcc cggggcgggg acgcgatagg cgaaggcggc cagcagcatg acggcaaagt
    11221 agaggaggcc gagcgtaagg aaggtggcgg cgacgccggt gctgccggtg ccgaccaggt
    11281 agacggtgtc ggcgtcgacg acgacctcgc gtagccggcc gccgaggctg gcgtagcgca
    11341 cgccgccctg ggtggtcagc gtcacatcgg cgagagcgcc gagcgattgc ggggcgcgcg
    11401 agtacagccc gagcaggaat tccttgagcg gcgcgccgat catggcgccg ccgccgaaac
    11461 ccatgatcgc catgccggtg gccatgccgc ggcggtcggg aaaccagcgg atgagcgtgc
    11521 tgacggggga gacatagccc aggccgaggc cgcagccgcc gatggcgccg tagccgacgt
    11581 agaggagcca gagctgatgg gtgacgatgc ccagcgaacc gagcaggaag ccgccgcccc
    11641 agcagcaggc ggccgtgacg ccgaccatgc gcggtccgac tttttcgagc cacttgccgc
    11701 cgacggccgc cgacaggccc agaaagacga tcgcaacggt gaagacccag acgacctggc
    11761 ccagggccca gtcggtcgag gcgctggtca cgacgccgtg gatccgcgtc agcggcgggt
    11821 tgaagatgct ccaggcgtag accgaaccga tgcagaggtg gatggcgatc gaggcgggcg
    11881 ggaccttcca gcgattaaag ccgggctcgg cgacgatgtg ctccttgctc aggcgcgaaa
    11941 agggaccgac catgaccggg gattccttgg gaggcttgcc ggccatccga tttaaggccg
    12001 gcgaaccgtg ggggatgaca cgccggcgga cagcctggag ccgggcagat caaggggcgg
    12061 cattatcaag aatacagcat ctgaatagca agaagtttga ttatccgttg acggcgaacg
    12121 ggacgacggc tggaggacgg gacgagggac gaggaattag cgacactcca tggagcgctg
    12181 tcgcgaatca gccgaaaagg atagaatcat tccggtctag tcgttccgga tcatcgtccc
    12241 ccggagttgc gcgtgatgct gctggattgg accggttcgc gctcaatcgg gcgcttcgtg
    12301 ctgctcggcg cgctggcctg ggtgcacgcc gccgccgcgg gcgagtcccc gacgatcgcc
    12361 gggcagccga tctttcccgc cgatcacatc tggaacacgc cggtcgacca gctcccgctg
    12421 gacagccagt cggcggtcta cgtggcgacg atcggcgccg aggcgacggt tcaccccgat
    12481 ttcggctcgg gtctttataa cggcggcccg atcggcatcc cgtatcttgt cgtcgaggcc
    12541 ggccagccga ccgtgccggt cagctttgaa tacgacgacg aaagcgaccc gggaccgtat
    12601 cccatcccgt ccgacgcgcc gatcgagggg ggtcacgcgg ccgacggcga ccgccacgtg
    12661 ctggtgctgg accgcggcag cgggttgctc tatgagctgt tcgcggcctt tccacagggg
    12721 ggcggcgaat cctggtcggc gggctcgggg gcgatcttcg atctgaacgg ttacgcgctg
    12781 cggccggccg ggtggacctc ggccgacgca gccgggctgc cgatcctgcc gggcctggtg
    12841 cgctacgacg aagtcgcggc cggcgagatc cgccatgcgt tgcgcttcac cgcgccgcag
    12901 acgcggcggg cctgggtctg gcccgcgcgc catttcgcct ccgacctgac cgggccggag
    12961 tatccgccga tgggccagcg gttccggctg cggtgggatt tcgatgaatc gggcttcgcc
    13021 cccgagtgcc aggtgatcct gcgcgcgttg aagcgctacg ggatgatcct ggccgacaac
    13081 ggttcggcgt ggttcatctc cggcgcgccg gatgaacgct gggacaatac ggcgctgcgc
    13141 cagttgcggc agatcccggg ccgcgagttc gaggcggtcg attgctccgg cctgatgctc
    13201 gccgaggatt ccgcgcaggc cctgcagccg caaaccggcc tgagcgccga ccggatggat
    13261 cgttaccgct gaatgaacct ggtgggcggc gttcgggaac tcccagacgc cgcaagagtc
    13321 ttacccgcgg ccgggggcgg agaagaccat ccgctgcgcc aggcgggtca atcccagggc
    13381 cagccctagc gcggcgaacg tgatccaggg atagtgcgcc ggagcggcga gcggggcatc
    13441 aagccggccc gggccacccc ccgcggcgag cagcgccgcg cgcgtgccga gtcggcccgc
    13501 cagcatcccg gccaggacta gcgcgagcat cagcccggcg ccccccgcgg ccagccgccg
    13561 gcgcggcagg gcgagcgtcg ccgccgccag catgaacagg aacatcacgg cgccgatcga
    13621 cgtcatccag accaggccga agccgccggg ccgcagcagc gtcgactgcg cgacgggggg
    13681 ctccaccagc atcagccaca agccggtggc cacctggaga aagatgccca gcgtggcgag
    13741 gagcgcgccg agtttggcca ggtcgcggcg ggcggcgccg gcgggcgagt cgctcggcgg
    13801 ggcgccgaag gcctcgccga cggccagcgc cagccccccc gccaccagtc cggccagcaa
    13861 ccgatggaaa aagcggcccc acagctcggg gccgggcagt cccgcgccgg acgtgctgag
    13921 cggctcggcg ctgaaggcga aagcgatccc cgccagcacg agcccatgca cgaacagggg
    13981 cagaagcttg ccgatgcgtc ggccggcgag cgccgcgccc caggtcagcg cggtcagggc
    14041 cgcgagcgcc agcgccagcg ggggtgtgcc ctcgggccac ggcaaggcga tccagacgag
    14101 gccggcgccc cccgcgccgg cgcgcatcag cccccagcgg acgccgaaga ggagcaactc
    14161 ggcggcgagc gcggcggtga gcgcaaccgg ggggatgcgc cgcaggcgcg acgcccgctg
    14221 gcgggcgacg accgcttcgc gttcgtcggc gggagggcgg tccagggcct gccccagcgc
    14281 cagcagcgcg gccaaccccg ccgcgcccag agcgacccag cgcagcgtct gaaccaggat
    14341 cagcaggacg gccatggccg cggccggacc ggtccaggcg agggtcagca gggcggcggg
    14401 gtcggcggcg ggcgattcca tcgggcgaac tccgggggga agggggcgct ggggggcggg
    14461 ggcgagccgg ggcggcgcgc ccgcaacggg cgcctgatta tcccacgcgg cggagagggg
    14521 ggaaaggcca agggacgcgg tcggcatgga aaaagacgac cggcggctca ttttcagctt
    14581 gcgcgcgggg atggggtttt ctaagatccg ggttcatgat gtagcacgtg ctaaatcaat
    14641 cagcaggcta tacagtcatg cccctccgtt ccttctttcc gcttctcctg ctcggtctgg
    14701 cgctggcgtg cgcctgcggc tcccccgaca cccccgataa cccggcttcg ggcgccgagc
    14761 cgtcccccca gaccgcgacg ttcgacggcg agtacccgat ccgcgtggtc acgacgaccg
    14821 gcatgatcgc cgatctggcg cgcgagatcg gcggcgagcg cgtccgggtc gaggcgctga
    14881 tgggccccgg cgtcgatccc catctctaca aggcctcgac cgacgatgtg agccggctgc
    14941 gcggcgccga cctgatcctc tataacgggc tgcacctgga gggcaagatg gccgacgtgc
    15001 tggagcgcct cagcaagcgc cagccgacgt tcgcggtggc cgatcaattg cccgcggaga
    15061 aactgcgcac gccgccggaa ttccagggtc tgcacgaccc gcacgtctgg ttcgacgtgg
    15121 cgctgtgggc gcgggtgggc gggcgcgtcg ccgacctgct ggcggagttc gacccggcca
    15181 acgcggcgga atacgccgcg cgcgcgaagg aatacctgga ccggctgaac gcgctggacg
    15241 cgtgggtcag agaccggatc gcgacgattc ccgagccgca gcgggtgatg atcacggcgc
    15301 acgacgcgtt cggttacttc ggcgccgcct acgacgtgga ggtgatgggc atccaggggc
    15361 tgagcaccga gtcggaggcg ggcgtgcggc gcgtgaacga actggtggac ctgctcgtcg
    15421 cgcgcgaggt gaaggcggtg ttcatcgagt cgagcgtctc ggacaagaat attcgcgcgc
    15481 tggtggaggg gagcgcggcg cgcgggcacg cggttcgcat cggggggcag ttgtattccg
    15541 acgcgatggg gcccgcgggc agcggcgccg accattacga aggcatgatc cggcacaatg
    15601 tggagacaat cgtcggcgcg ttgcgctagc gggagcggcg gtctgcgtgg cgcggacttt
    15661 tcgaccgaat cggaccgacg cgacggatga caagaacagg actcagcgaa cgatgaaggc
    15721 aaccgcggac aaggccaccg gcgcggaacc gcccttgcgg gtgcgcgatc tgaccgtcgc
    15781 ctaccatcgc aagccggtgc tgtgggacgt ggaactgacc gcgcccgagg ggaagctgat
    15841 cggcatcatc gggcccaacg gcgcgggcaa gagcacgctg atcaaggcgg tgctggggct
    15901 ggtgccgcgc gccagcggcg aagtgctggt ctacggcaag ccgtatgagg ccaaccggcg
    15961 gctggtgggc tacgtccccc agcgcggcag cgtggactgg gactttccgg tcgacgcgct
    16021 cgacgtggtg ctgatggggc gctacgggcg gctggggtgg atccgccggc cggggcgcgc
    16081 cgaccgccag gccgcgatga actgcctgga gaaggtcggg atggcggcct atgcgcaccg
    16141 gcagatcagc cagctctccg gcgggcagca gcagcgcgtg ttcctggcgc gggcgctggc
    16201 gcaggaggcg cagctctatt tcatggacga accgttcgcg ggcgtggacg cggcgaccga
    16261 gaaggcgatc gtggcgctgc tgcatgagtt gaaggcgtcg gggcggacct gcctgatcgt
    16321 gcatcatgac ctgcagacgg tgagcgagta tttcgatcat gcgattctgt tgaacatgcg
    16381 cgtcgtggcg cagggaccga ccgcggaagt ggtgacgcgg gacaatctgc acaagacgta
    16441 cggcgggcgg ctgacgctgc tggaagaagc cgcgaccgag atggcccgcg cccggcggta
    16501 ggtgagcaga agcgatatcg ggactgggat cgggatcggc atcggagtcg gattacgaaa
    16561 gccgaggacg agggacgaca tcgagcgtga actggctggc ttacaatacg ctcatcgtgc
    16621 tggccggcac ctcgctgctg ggcatcgtcg ccggggtgct gggcagttac atggtgctgc
    16681 gccggcgggc gctgctgggc gacgcgctct cgcacgcggc gtatccgggc ctgggtctgg
    16741 cgttcctgat caccggtcag cgccacttcg gcgggctgct gaccggcgcg ttcgcgacgg
    16801 cgctgatcgg gtcgtggacg gtcggggcga tccggcgcca cacgcgcgtg aaggaggacg
    16861 ccgcgatcgg gatcgtgctg agcgtctttt ttgggctggg cgtggtggtc tcgcgcatcg
    16921 tgcagaacac gacgacgacc ggtagcaagg cggggctcga cagcttcata ttcggcaagt
    16981 cgtcggggat ggtggcggcc gacgtgcggg cgatcctgct ggtgggggtg gtggtgctgg
    17041 cggcgacgct gctgctggcg aaggagttcc ggctgctggc gtttgatccc gatttcggcc
    17101 gcgtgcaggg ctggccgatc ctgctgctcg acatcattat gatgagcctg gtgtgcgtga
    17161 cgacgatcgt cgggctgccg gcggtggggg ttgtgctgat ggcggcgctg ctgatcatcc
    17221 cgggggcggc ggcgcggttc tggaccgacc ggctggggcg catgctgtgg ctggcggggc
    17281 tgttcggcgg cgtggcgggg gcgtgcggga cgctggccag cgcctgggcg ccgtcgaatg
    17341 tcggcgcggg gccggtgatc atcctgacgg cggccggctg tttcctgttt tcgatgctcg
    17401 ccgcgccgcg ccggggggcg gtggcgcgcg tgctgcgcca tgtcgcgctc gcgcggcgcg
    17461 tggcgatgca gaacctgctg cgcacgcttt acgagctgag cgaaccggcg tggcccgacc
    17521 ccgcgccgct gcggccgggg gatctgaccg ccgcgcgctc gtggtcggcg gcgcaggcgc
    17581 ggcggttgtt gcggcgggcg gccgcgcgcg gctgggttga gccgaacggc gacggctggc
    17641 ggctgaccgg cgccggctgg cggcgcgcgg ccgaggtgac gcgcgcgcat cggctgtggg
    17701 agatctttct gattgaggaa gcgcaggtgg cgccgacgct ggtcgaccaa ctgtcccagc
    17761 gcggcaccga atgcagcggc g
//
LOCUS       DAOPCO010000051        17477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3327_length_17477_cov_5.509069, whole genome shotgun sequence.
ACCESSION   DAOPCO010000051 DAOPCO010000000
VERSION     DAOPCO010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 17477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17477
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3327_length_17477_cov_5.509069"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PLS90_05740"
     CDS             complement(<1..1082)
                     /locus_tag="PLS90_05740"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK01940.1"
                     /translation="MCIACATSPLDAARKDAFGDALLAMLNGGMTCLMISLGHRAGLF
                     DAMADGEPRTLAMLVDQTGLNERYVREWLGAMVAARIIERDPQTGMHRLPPEHAHWLS
                     RNNPTGNMAVFAQYLAVLGSVEDDVLECFRRGGGVPYAAFKRFHDVMAEDSGQAIVQT
                     IVDQVVPRMPGLHERLQAGIDVLDVGCGRGLAMMQLAAAYPRSRFTGYDLSEAAVAEA
                     RRVAAERGLSNIRFEQRDLTDWDEPASYDWITALDAIHDQARPDKVLAAIRRALRPAG
                     VFLMMDIDASSEPVDNIDHPLGAMIYAISCMHCMTVSLAQGGMGLGAAWGVQLAERML
                     REAGFESVRIERMAHDFQNAYFTMET"
     gene            complement(1169..1765)
                     /locus_tag="PLS90_05745"
     CDS             complement(1169..1765)
                     /locus_tag="PLS90_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK01941.1"
                     /translation="MKSPTSHSPVRQRILDAAADLFYRQGYRATGINQIIAESGVAKA
                     SFYDHFPSKDDLLRAYAIEMSRKEFELVRQEVDAFPTARERFFGPLTILPPWFESSDY
                     RGCPFQIMVAEAPPDSAGVREVARRHRETFRRYLHQLTGALMVEEPTLARADAAMLVD
                     SYLLLFEGSIALAVAYRESWPVERAITTLETLLDSQRR"
     gene            2124..2810
                     /locus_tag="PLS90_05750"
     CDS             2124..2810
                     /locus_tag="PLS90_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPK01942.1"
                     /translation="MTSHPRLLAMIPARLGSQRLKQKNLREIAGASLVAWAVRKCRAA
                     GVFDEIWVNSEAEEIGRIAEAEGARFHRRPPELANNVATSEQFVHEFLLAHPCDYLIQ
                     VHSIAPLLTAQEVRAFVEAMLADGCDVQLSCTLEQIECALDGRPVNFTFAEKTNSQEL
                     RPIQRVTWSITGWRRATYLEAFAAGRTATYAGRVAFHPLDRLAGHIIKTEEDLQQAAA
                     LWPLRFGARD"
     gene            complement(2857..4941)
                     /locus_tag="PLS90_05755"
     CDS             complement(2857..4941)
                     /locus_tag="PLS90_05755"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK01943.1"
                     /translation="MAEPTPPPQSASDQPDEATDDSPPAARPAPTERGAPPPVASLIW
                     LQPLSLALLAIAAFCPVLSNGFLLGNELYPPGEPTAPAPDTWRHAESLLTAGHHGRLG
                     HLFLRVEGRLLGDRAAAWHLVSLLLHAANAVLLWLLLHRLRVRAAWFAAALYAVHPLH
                     AQAIALISEQPRLLAAAAALLGALGWVRFAARPRWPLYAGALALLAIALAIHASVWPV
                     PFVLAVLALWRRERWSVLPLGRYHLLLAPAMLLAAWVALADAAPVQEHLVSIAHVSPP
                     ERLLIAARAVGLHALHVVWPAGLAAFYPQWTVALTPPNLIPIGLLILAFVVLLVLRLA
                     GRAGNEPLAALATYLLLLAPALGLFDRSILRDAYVADRFQYLAAAVPLAALAAILAGL
                     FARLPASLRDVRRAGAVTLLVLLAVISLRQLGPYRDPDPLLRHSLARHPESAAAHTLL
                     ATHLRDRQQLDAAAAEYARAVDLRPRDARLRNALGATLMVLKRNEEAERELREALRLD
                     PGSTPAATNLGLLLIGLGQFAEAEQLLIRTLAVKPDDVQLHRALSDLLLAQQRFSEAE
                     DHLRRILDLAPEQPQAHAVLGDLLVQLNRPDEAIARYEAALKLEPRLIDARFNLANLL
                     AEQGRFDEAIGHYRNVLELQPDAGAVHLNLATALELTGARQQALEHYAEVQRLAPDLI
                     TPAIRRRIQSLY"
     gene            complement(5118..6257)
                     /locus_tag="PLS90_05760"
     CDS             complement(5118..6257)
                     /locus_tag="PLS90_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK01944.1"
                     /translation="MKIRPLNALLLHVVVLLAGVAFAFPLAWMVLTALKPLEQTMTLP
                     PVWLPHRLYLPDTDPPVEARQVGRFERPMVRIERDDTGETEWVPREYVSGGELRVISS
                     TGEETARFVHVLEEIDASPEAPWYRLRAIHPDGSLGEERLLPADQVTRRISLRWDNFI
                     LALRAMRMFPRYLANTVVLCLLTVCGTVISCALVAYGFSRIDWPGRDRLFILALATMM
                     IPFPVVMVPLYGLFRWLGWIGSLKPLWVGAWFAPPFYVFLLRQFFLTIPAALADAARI
                     DGCNEFAIFRRVLLPLVKPALLVVALFQFMATWNDFLGPLIFLTDQRDFTLALGLQYF
                     QSQHGGTEWHYLMAASTVVILPVIVLFFFTQRTFIEGISMTGLKE"
     gene            complement(6254..7147)
                     /locus_tag="PLS90_05765"
     CDS             complement(6254..7147)
                     /locus_tag="PLS90_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006208672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK01945.1"
                     /translation="MRTAAKLRLRNGLLFISPWLLGFALFVVYPVGATFYYSFCDYDV
                     LSHPVWLGLQNYRDMLTDTVLWQALGNTLLYAAVSLPLGLALSLGLALLLNQPIVLRP
                     LFRTIFFLPSLVPAVALAMLWLWMFNGQFGLLNIALETFGIKGPIWLGDPAWTKPALV
                     IMSLWGVGGGMIITLAALQEVPRELYESAEIDGARARHKLWHITLPMISPVLYFNLIM
                     GLIGVMQIFAQPYIMFGGNGGPSRSALFYAVYLYENAFTYNDMGYACAMAWILFLIIL
                     GLTLIATRATRRHIYYAGKAK"
     gene            complement(7144..8568)
                     /locus_tag="PLS90_05770"
     CDS             complement(7144..8568)
                     /locus_tag="PLS90_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK01946.1"
                     /translation="MSYRRCIASARVAAWSLAALLLGCGATASRQAEGGPVQIEYWEK
                     WTGHEGEAMREIVDDFNASQNEIHVNLITVSQIERKIMLAIMGGHPPDVAGLWTHWLH
                     ILCERNALHPLDRLIAEAGLSLDDYLPVFKVLLSDRGFIFALPTSGTSVALYRNRQLF
                     REAGLDPDHSPTSIAELDAIAERLTVVEQRRDGKIVRLPYTELTAAEKEARNFRIVQM
                     GFAPAEPGWWTMLWSTWFGGALWDGEEKITADTPENRAALEWFRSYPLKYGLDNMRAF
                     ASGFGVFMSPQNPFYSGKIAMVLQGVWMPNFIEKYAPDLDWAAAPFPAVDPAMNPITI
                     VESDIVMIPRGARHPREAFRFMTYLQRPEVIERLCLAQRKMSPLRQASPHYLETHKNP
                     FLPMFIEIARGPGARIAPRVPVWYEYMAEINAAYDKVGSLTATPAEALAQVQRRVEWK
                     QRRAQVRWDAVGPARLREWSEWQP"
     assembly_gap    9165..9264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9265..10551)
                     /locus_tag="PLS90_05775"
     CDS             complement(<9265..10551)
                     /locus_tag="PLS90_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPK01947.1"
                     /translation="MRPRSFDDWIGQAEVAGPDSPLRRMLARGALHSLILWGPPGCGK
                     TTLARLIAGQTDMAFQPLSAVISGIKEVKAVIDQAKARLALKNRRTILFIDEIHRFNK
                     AQQDAFLPHVENGTILLIGATTENPSFSVIAPLLSRCRVLTLRPLDVGELVTLLERAL
                     RDAERGLGAGGWRAEPEVLERIAGLSDGDARRALSLLEQCVALAEADPSLLAGDARKL
                     TVASLAAMLERKHLLYDRGGEEHFNLISAFHKSMRASDPQGAVYWLFRMLEAGEDPLW
                     IARRILAAASEDVGMADPQALQVAVAAFQAFQALGLPEGELPLTHAAIYVATAPKSNS
                     VNVARGQARDAVRRHGSLPVPLHLRNAPTGLMKQLGYGREYLYDHDQPDHFSGQPTLP
                     DELEAQEVKFYEPGQFGFEKEIRKRMDWWDAKRAERR"
     gene            10862..11248
                     /locus_tag="PLS90_05780"
     CDS             10862..11248
                     /locus_tag="PLS90_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPK01948.1"
                     /translation="MNISPGKRQLLVVGDRVLIKPQTGDERTDSGLILPQTVADRAQV
                     NSGRVVAIGPGTPMPFGEEDHDEPWRQVERRPRYIPMQAKVGDLALFLRKATVEIKYE
                     GETYLIVPQSAILVLLRDEDEALFAD"
     gene            11268..12230
                     /gene="miaA"
                     /locus_tag="PLS90_05785"
     CDS             11268..12230
                     /gene="miaA"
                     /locus_tag="PLS90_05785"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPK01949.1"
                     /translation="MAEPARLPALLLSGPTGIGKSALALRLADELPLEIVSADSMQIY
                     RGLAIGTAQPSAEERRRVPTHLVGELDPTERFDARRFVERADQLRREIHARGRLPVFV
                     GGTGLYLRALRWGLFEGGGRDEAVRAALAAEARQAGSAALHARLARVDPAAAERIAPN
                     DAVRIVRALEVHVLTGRRLSELQRQWTAPAPRFPHLLVVLFERRDRLRERIARRADAM
                     LAAGWIDEVAELLARGIPPEAHCFRALGYREIIAHLRGELSRDALAERIATATVQFAK
                     RQMVWFRRERPAIWLPAGDGDAGERVADLAWLARRLLAECGAAR"
     gene            12597..12806
                     /gene="hfq"
                     /locus_tag="PLS90_05790"
                     /pseudo
     CDS             12597..12806
                     /gene="hfq"
                     /locus_tag="PLS90_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
     gene            13197..14174
                     /locus_tag="PLS90_05795"
     CDS             13197..14174
                     /locus_tag="PLS90_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPK01950.1"
                     /translation="MNRIRGARRIQVVGHVRPDGDCIGALLATHHLLAQWGIEHALAA
                     AAMPISAYDDLAGFERIAEQPAGWRPDLVVFVDTADLARGLPGWTPGAPIVNIDHHAG
                     NTRFGEINWIEPACSSVCEMLFWLARRAAARIDCDLAEALLLGLMTDTGSFRFNNTGA
                     TQLRVAAELVEAGARPDGIARLAWGSQTFEGLSLLGTVLSRIRLLCGGRLSWSELRRA
                     DYARVGGEQHAPENLADQLRAVRGVRLGLLFHELPDGAMRINYRSDGAVNVSALATAL
                     GGGGHPAAAGAYLTGADYEAVRDRAIADARQALEAALGEAPGASEPTGG"
     gene            14206..15054
                     /locus_tag="PLS90_05800"
     CDS             14206..15054
                     /locus_tag="PLS90_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK01951.1"
                     /translation="MIIDFHTHAFPDGLAERAMAQLAPAAGDAFRPHHDGTLGGLLRL
                     MDAAGVDRAVVASIATKPAHAGPILDWSLRIASDRIIPFASVHPDDGEAVRRVEAIAA
                     AGLAGIKLHPFYQRFTVDEARLDPIYDAARANGLIVLLHAGYDVAFPRDPLATPAATA
                     RVVARHPGLKLVAAHLGGWMDWERARADLVGRPCWLDLSSSLEFLTPAAARDLIERHG
                     ARRVLFATDAPWSDPARTLARLRALELDPRQEEWILSANARELLAEDAREAQAAPPTP
                     NQVPSS"
     gene            15061..15366
                     /locus_tag="PLS90_05805"
     CDS             15061..15366
                     /locus_tag="PLS90_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01952.1"
                     /translation="MGRCVSPEYFQRHDSSPPYFEEPTTITHWLRIGSLEFHPTAMPV
                     ETGVKEWRQYVQHGGLLSGVPTQFGGASPGLTTTAALLRRWLPGPWRLAPLFRPARE"
     gene            complement(15654..16844)
                     /gene="fdhA"
                     /locus_tag="PLS90_05810"
     CDS             complement(15654..16844)
                     /gene="fdhA"
                     /locus_tag="PLS90_05810"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HPK01953.1"
                     /translation="MASNRGVVYLKQRHVEVQSIDYPKLALGNRKCEHGVILKIITTN
                     ICGSDQHMVRGRTTAPPGLVLGHEITGDVIECGRDVEFIKQGDIVSVPFNIACGRCRN
                     CKEGKTGICLNVNPARPGAAYGYVDMGGWVGGQAEYVMVPYADFNLLKFPDKDQAMAK
                     IRDLTLLSDIFPTGYHGAVTAGVGPGAIVYVAGAGPVGLACAASCQLLGAACVIVGDM
                     IPERLEQARRFGCETIDLRQKASLAEQVEQITGEPEVDYAVDCVGFEARGHGANANTE
                     QPATVLNAVMDITRAGGGIGIPGLYVTGDPGGVDENAKIGMLGIRIGLGWAKSHHFHT
                     GQCPVMKYNRQLMNAILYDKIQIADAVNVTLISLDDAPKGYDEFDKGAAKKYVIDPHG
                     SVAA"
BASE COUNT         2801 a         5656 c         6287 g         2633 t
ORIGIN      
        1 cacgtctcca tggtgaagta ggcgttctgg aaatcgtgcg ccatgcgctc gatgcggacc
       61 gattcgaacc cggcctcgcg cagcattcgt tcggccagtt gcacgcccca cgcggcgccg
      121 aggcccatcc cgccctgcgc gagcgagaca gtcatgcagt gcatgcacga gatggcatag
      181 atcatggcgc ccagggggtg gtcgatgttg tcgaccggtt cactggaggc atcgatgtcc
      241 atcatcagga agacgccggc ggggcgcagg gcgcggcgga ttgccgccag caccttgtcg
      301 ggccgggcct ggtcgtggat cgcgtcgagc gccgtgatcc agtcgtagct cgccggctcg
      361 tcccagtcgg tcaggtcgcg ctgctcgaag cggatgttgg acagtccccg ctccgcggcg
      421 acgcgccggg cctcggccac ggccgcctcg ctcaggtcgt agccggtgaa ccggctccgc
      481 ggataagccg ccgccagttg catcatcgcc aggccacggc cgcagccgac gtccagcacg
      541 tcgatgcccg cctgcagccg ctcgtgcagg cccggcatcc gcggcacgac ctggtcgacg
      601 atcgtctgca cgatggcctg gccgctgtcc tcggccatca cgtcgtggaa gcgcttgaag
      661 gcggcgtagg ggacgccgcc gccgcggcgg aagcactcga gcacatcgtc ctcgacactg
      721 cccagcacgg ccagatactg cgcgaagacc gccatgttgc cggtcgggtt gttgcggctg
      781 agccaatggg cgtgttcggg gggaagccgg tgcatcccgg tttgcgggtc gcgctcgatg
      841 atgcgggcgg cgaccatcgc gccgagccat tcgcgcacgt agcgctcgtt caggccggtc
      901 tggtcgacca gcatcgccag ggtgcggggt tcgccgtcgg ccatggcgtc gaacaggccg
      961 gcgcggtggc cgagcgagat catcaggcag gtcatgccgc cgttgagcat ggcgaggagg
     1021 gcgtcgccga aggcgtcctt gcgggcggcg tcgagggggg aggtggcgca ggcaatgcac
     1081 atggtgcgga tcccttcgta aaagatgatc ggcctatcat gaataaagac agaccgttat
     1141 gtctgctctt cgcgtcaaaa aagaagagtc agcggcgctg gctgtccagc aaggtttcca
     1201 aggtggtgat ggcccgttcc accggccagc tttcgcgata cgcgacggcc agcgcgatgg
     1261 aaccctcgaa cagcaacagg tagctgtcga cgagcatcgc cgcgtcggcc cgcgccagcg
     1321 tcggttcttc caccatcagg gcgccggtca gttggtgcag gtagcggcgg aaggtctcgc
     1381 ggtgccggcg cgcgacttcg cgcacgccgg ccgaatcggg cggcgcctcg gcgaccatga
     1441 tctgaaacgg gcagccgcgg taatcgctcg attcgaacca gggcggcagg atggtcaggg
     1501 gcccgaagaa ccgttcgcga gcggtcggaa aggcgtcgac ttcctggcgg acgagttcga
     1561 attccttgcg cgacatttcg atggcgtagg cccgcagcag gtcgtctttg gaggggaaat
     1621 ggtcgtagaa cgacgccttg gcgacacccg actcagcgat gatctggttg atgccggtgg
     1681 cgcggtagcc ctggcggtag aacaggtcgg cggcggcgtc gaggatccgc tggcggacag
     1741 gggagtggga cgtcggcgat ttcatggtgg gggagaatat gacagaccgt tctgtctgtc
     1801 aacgagaaaa atggggctgg cgaaccggca gccccgtttc aagcgcggcg cccgagcgtt
     1861 gccacgccac acggcgacac cggtacggtt ccgaggaacc gccgaatcgg taacgatacc
     1921 gatttcgatg gcgatcccga tcccgatccc gacaactccg gttctccttt gcggctcggc
     1981 ccctgcgctt ccatggccga cgtcaatttt ccgggcgatt ctcagtggag cgcggcgggc
     2041 ggaatcctta tcttgatcac ccgggtcggg cgcgccgccg cgccctgccc ctcgaccggt
     2101 caaactcgaa ggatggagtc accgtgacct cgcaccctcg ccttctcgcc atgatccccg
     2161 cgcggctggg cagccagcgg ctgaaacaga agaacctgcg tgaaatcgcc ggcgcgtcgc
     2221 tggtggcgtg ggcggtgcgc aagtgccgcg ccgccggcgt gttcgacgaa atctgggtca
     2281 attccgaggc ggaggagatc ggccggatcg ccgaggccga gggggcgcgg ttccaccgcc
     2341 gtccgccgga gctggccaac aacgtcgcca cttccgagca gttcgtgcac gagtttctgc
     2401 tggcgcaccc gtgcgactat ctgattcagg tccattcgat cgcgccgctg ctgacggcgc
     2461 aggaggtgcg ggcgtttgtc gaggcgatgc tcgccgacgg ttgcgacgtg cagctcagtt
     2521 gcacgctgga gcagatcgag tgcgcgctgg acggccggcc ggtcaatttc acgtttgccg
     2581 agaagaccaa ctcccaggag ctgcggccga tccagcgcgt gacgtggagc atcaccggct
     2641 ggcggcgcgc gacctacctg gaggcgttcg ccgcggggcg gaccgccacc tatgccggcc
     2701 gcgtcgcgtt ccatccgctg gaccggctgg ccggtcacat catcaagacc gaagaggacc
     2761 tgcaacaggc cgcggcgctc tggccgctgc gtttcggcgc gcgggattga tcggccgcgg
     2821 gcgcggccgg tttgaaccga tggcggcgga ggccgctcag tagaggctct gaatccgccg
     2881 ccggatggcc ggcgtgatca ggtcgggggc caggcgctgg acctcggcgt agtgctccag
     2941 cgcctgctgc cttgcgccgg tcagttccag cgccgtggcc agattcaagt ggaccgcccc
     3001 cgcgtcgggc tggagctcaa gcacgttgcg gtagtgcccg atggcctcat caaagcgccc
     3061 ttgctcggcg agcaggttgg ccaggttgaa gcgggcgtcg atcagccgcg gctcgagctt
     3121 cagggcggct tcgtaccgcg cgattgcctc atcgggccgg ttcagttgaa cgagcagatc
     3181 gccgaggacg gcgtgcgcct ggggctgctc cggcgccaga tccaggatgc ggcgcaggtg
     3241 gtcctcggct tcggagaacc gctgctgcgc gagcagcagg tccgacagcg cgcggtggag
     3301 ctggacgtcg tcgggcttca cggcgagcgt ccggatcagc aactgttccg cctcggcgaa
     3361 ttgccccagg ccgatcagca gcaggccgag gttggtcgcc gcgggggtgg agccgggatc
     3421 gaggcgcagg gcctcgcgca gctcgcgctc ggcttcctca ttccttttca agaccatcaa
     3481 ggttgcgccc agggcgttgc gcagccgcgc gtcgcggggg cgcagatcga ccgcgcgggc
     3541 atactccgcg gccgccgcgt cgagctgttg ccggtcgcgc aggtgcgtgg ccagcagcgt
     3601 atgggccgcg gccgactcgg ggtggcgcgc caggctgtgg cgcagcagcg ggtcgggatc
     3661 gcggtaaggc cccagttggc gcaggctgat gaccgccagc aggacgagca gggtgaccgc
     3721 gccggcgcgg cggacatcgc gcagcgacgc cggcaagcgc gcgaacaggc cggccaggat
     3781 cgcggccagc gccgccagcg gcacggcggc cgccaggtac tggaaccggt cggcgacata
     3841 cgcatcgcgc aggatcgagc ggtcgaacag cccgagcgcc ggcgccagca gcagcaggta
     3901 ggtcgcgagc gccgccagcg gttcgttccc cgcccggccg gccaacctca gaaccagcag
     3961 gaccacgaac gccaggatca gcagcccgat ggggatcagg ttgggcggcg tcaacgcgac
     4021 ggtccattgc gggtagaacg cggccaggcc ggcgggccag accacgtgga gcgcatgcag
     4081 gccgacggcg cgggccgcga tcagcagccg ctcgggcggg ctgacgtggg cgattgaaac
     4141 caggtgctcc tggaccggcg ccgcatcggc gagcgccacc cacgccgcca gcagcatcgc
     4201 cggcgccagc agcaggtggt agcggccgag cggaagcacc gaccaccgtt cgcggcgcca
     4261 gagcgccagc accgccagca cgaacgggac cggccagacc gaggcgtgaa tcgcgagcgc
     4321 gatcgccagg agcgccagcg ccccggcgta gagcggccag cgcggccgcg cggcaaaccg
     4381 gacccagccg agggcgccca gcagcgcagc ggccgccgca agcaggcggg gttgttcgct
     4441 gatcagcgca atcgcctgcg cgtggagcgg gtgaacggca tacagcgccg cggcgaacca
     4501 ggcggcgcgc acgcgcaagc ggtgcagcag gagccagagc agcacggcgt tggcggcgtg
     4561 cagcaacagg ctgaccagat gccaggcggc ggcgcggtcg cccaagagcc ggccctccac
     4621 ccgcaggaag agatggccga gccggccgtg atgccccgcc gtcaggaggg attcggcgtg
     4681 gcgccaggtg tcgggcgccg gggcggtcgg ttcgcccggc ggatacaatt cgttgccgag
     4741 cagaaagccg ttggaaagca cgggacagaa cgcggcgatg gccaggagcg cgagcgagag
     4801 gggttggagc cagatcaggg atgccaccgg gggcggcgcg ccgcgttcgg tgggcgccgg
     4861 ccgcgccgcg ggcgggctgt cgtcggtcgc ctcatccggc tgatcggagg cggactgggg
     4921 cgggggcgtc ggctcggcca ttcggtgaac ggggcatcct tccacgcggg gatggaggca
     4981 ttgtggcacc cgggtggaag cgttgtcatc aggcaaacga caatccggcc gagaacaatt
     5041 aaaaccagtc gggattcaag cagatggaga tccgtcggat cggtcggatg cgaccgatcg
     5101 cggccgccgg cccgcgctca ctccttgagc ccggtcatcg agatcccttc gatgaacgtg
     5161 cgctgggtga agaagaacag tacgatgacg ggcaggatga cgaccgtgct ggccgccatc
     5221 agatagtgcc attcggtgcc gccgtgctgg ctctggaagt actggagccc gagggccagc
     5281 gtgaagtcgc gctgatcggt caggaagatg agcggcccga ggaagtcgtt ccaggtcgcc
     5341 atgaactgga acagcgcgac gacgagcagg gccggcttga ccagcggcag cagaacgcgc
     5401 cggaagatgg cgaactcgtt gcagccgtcg atgcgggcgg cgtcggcgag cgccgccggg
     5461 atcgtcagga agaactggcg caggaggaag acgtaaaacg gcggggcgaa ccacgcgccg
     5521 acccacagcg gcttcagcga cccgatccag ccgagccagc ggaagaggcc atagagcggg
     5581 accatgacga cgggaaacgg gatcatcatc gtggcgagcg ccaggatgaa cagccggtcg
     5641 cgcccgggcc agtcgatgcg ggagaagcca taggccacca gcgcgcagga gatgaccgtg
     5701 ccgcagacgg tcagcaggca gaggaccacc gtgttggcca ggtagcgcgg gaacatgcgc
     5761 atcgcgcgca gggcgaggat gaaattgtcc cagcgcaggc tgatccggcg ggtcacctga
     5821 tcggcgggca ggagccgttc ctcgccgagc gagccgtcgg gatggatcgc gcgcaggcgg
     5881 taccacggcg cctcgggcga ggcgtcgatc tcctccagga cgtgcacgaa gcgggcggtt
     5941 tcctctccgg tgctggagat gacgcgcagt tcgccgccgc tgacgtattc gcgcgggacc
     6001 cattcggtct cgcccgtgtc gtcccgctcg atccggacca tcgggcgctc gaagcggccg
     6061 acctggcgcg cctcgaccgg cgggtccgtg tcgggcaggt agagccggtg gggcagccag
     6121 acggggggca gcgtcatcgt ctgctcgagc ggcttgaggg cggtcaggac catccacgcg
     6181 agtgggaagg cgaaggccac gccggccagc agcacaacga cgtgaagcag cagggcgttc
     6241 agcgggcgga ttttcatttc gccttccccg catagtagat atggcgccgg gtggcgcgcg
     6301 tggcgatgag cgtcaggccc agaatgatca ggaacaggat ccacgccatc gcgcaggcgt
     6361 agcccatgtc gttgtaggtg aaggcgttct catacagata gacggcgtag aacagggcgg
     6421 aacggctggg accgccgttg ccgccgaaca tgatgtaggg ctgggcgaag atctgcatca
     6481 caccgatgag ccccatgatc aggttgaagt agagcacggg cgagatcatc ggcagcgtga
     6541 tgtgccacag cttgtggcgc gcgcgcgcgc cgtcaatttc ggccgattca tacaactcgc
     6601 gcgggacctc ctgcagcgcg gcgagcgtga tgatcatgcc gccgccgacg ccccacaggc
     6661 tcatgatgac cagcgccggc ttggtccagg cgggatcgcc cagccagatc ggccccttga
     6721 tgccgaacgt ctccagcgcg atattgagca ggccgaactg gccgttgaac atccagagcc
     6781 agagcatcgc cagggcgacg gccggcacca gcgaggggag aaagaagatc gtgcggaaca
     6841 gcggccgcag cacgatcggc tggttgagca gcagcgccag ccccagcgac agcgccagcc
     6901 ccaacggcag actgacggcg gcatagagca gcgtgttgcc gagcgcctgc cacagcacgg
     6961 tgtcggtcag catgtcgcgg tagttctgca agccgagcca gacgggatgc gagaggacgt
     7021 cgtaatcgca gaacgaataa tagaacgtgg cgccgacggg atagacgacg aacagggcga
     7081 aaccgagcag ccacgggctg atgaagagca ggccgttgcg caggcggagc ttggcggcgg
     7141 tcctcatggt tgccactcgc tccattcgcg caggcgggcc gggccgacgg cgtcccagcg
     7201 gacctgggcc cggcgctgct tccactcgac acggcgctga acctgcgcca gcgcctcggc
     7261 gggggtggcc gtcagcgagc cgaccttgtc gtaggccgca ttgatttcgg ccatgtattc
     7321 gtaccagacg gggacgcgcg gcgcgatgcg cgcgccgggc ccccgcgcga tctcgatgaa
     7381 catcggcaga aacggattct tgtgcgtctc gaggtagtgc gggctggcct ggcgcagcgg
     7441 cgacatcttg cgctgcgcca ggcacagccg ctcgatcacc tccggccttt ggaggtaggt
     7501 catgaagcgg aaggcttcgc gcggatggcg ggcgccgcgc gggatcatca cgatgtcgct
     7561 ctcgacgatc gtgatcggat tcatcgccgg gtcgacggcg gggaacggcg cggccgccca
     7621 atccagatcg ggggcgtatt tttcgatgaa gttgggcatc cagacgccct gcagcaccat
     7681 cgcgatcttg cctgaataga aaggattctg cggcgacatg aagacgccga acccggaggc
     7741 gaaggcgcgc atgttgtcca gcccgtattt gagcggatag gagcggaacc attccaacgc
     7801 ggcgcgattt tccggcgtgt cggccgtgat tttctcctcg ccgtcccaca gcgcgccgcc
     7861 gaaccaggtc gaccacagca tcgtccacca gcccggctcg gccggcgcga agcccatctg
     7921 gacgatgcgg aaattgcgcg cctccttctc cgcggcggtc agttcggtgt agggcagccg
     7981 gacgattttg ccgtcgcgcc gctgctcgac gacggtcagc cgctcggcga tggcgtccag
     8041 ctcggcgatg ctggtgggtg agtggtccgg gtcgagcccc gcctcgcgga agagctggcg
     8101 gttacggtag agcgcgacgg aagtgcccga ggtcggcagg gcaaagataa agccgcgatc
     8161 cgacagcagg accttgaaga ccggcaggta gtcgtccagc gagaggccgg cttccgcaat
     8221 cagccggtcg agcgggtgca gcgcattgcg ttcgcaaagg atatgcagcc agtgcgtcca
     8281 gaggccggcg acatcgggcg gatggccccc catgatcgcg agcatgatct tgcgctcgat
     8341 ctggctgacg gtgatcagat taacgtggat ttcgttctgc gaggcgttga aatcgtccac
     8401 gatttcgcgc atcgcctcgc cctcatggcc ggtccacttc tcccagtatt cgatctggac
     8461 cgggccgccc tcggcctggc gcgacgcggt tgcgccgcaa ccgagcagca gggccgcaag
     8521 ggaccacgcg gccacgcgag ctgaggcgat gcaacgacgg taagacatag gatcgatcgg
     8581 tttggctcct gactacggac cctggggtcc ttatcatgcc agcagaaatc cgccagtcaa
     8641 gcgttgcgcg acggcggagg atgaaaaaaa cgcggaggac gtgctgggac ttcacgagac
     8701 caggccgttc tgaaaacaag gaaaatgatc tcacacagag acgcggaggc gccgaaagtg
     8761 gatctgaaat tcgaacggcc gaggccttgc cgattcgatc agcaagccag tcccggcagg
     8821 gagggcatac ctcagcctgc cagttcagtg gcggacaccc gggaattaag aagggaaaga
     8881 gcccgcaggg agggctgaac ggctcgatcc gatcgcctgt atgtgggatg agaggtgata
     8941 tttgactgag cttttcccga cgtcttcggc gttattctga aaggatgaaa acagtctggc
     9001 ggagattcgc ccccttcgga gacggggcgg ctcacgctga cggctgttga cggaattgag
     9061 tcggcggcgg tcgggaatcg gacgagaaca agcaatgggg ctgtcagccg cgccgttccg
     9121 cgcgtttggc gtcccaccaa tccatgcgtt tgcggatttc ctttnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnngcgccg ttccgcgcgt ttggcgtccc accaatccat
     9301 gcgtttgcgg atttcctttt caaacccgaa ctggcccggc tcgtagaatt tcacctcttg
     9361 cgcttccagt tcgtcgggca gcgtcggctg gccggagaaa tgatcgggct ggtcgtggtc
     9421 atacaggtac tcgcgcccgt agcccaactg cttcatcaag ccggtcgggg cgttgcgcag
     9481 gtgcagcggc acgggcaggc tgccgtggcg ccggacggcg tcgcgcgcct ggccgcgcgc
     9541 cacgttcacg ctgttgctct tgggcgccgt cgcgacgtag atcgccgcgt gcgtcagcgg
     9601 caattccccc tccggcaggc cgagcgcctg gaacgcctga aacgccgcca cggccacctg
     9661 cagcgcctgc ggatcggcca tgccgacgtc ctcggaggcg gccgccagga tgcgccgcgc
     9721 gatccacagc gggtcctcgc ccgcttcgag catgcggaac agccaataga cggcgccttg
     9781 ggggtcggag gcgcgcatgc tcttgtggaa cgcgctgatc aggttgaagt gttcttcgcc
     9841 gccgcgatcg tagagcagat gcttgcgctc gagcatcgcc gccaggctcg cgaccgtgag
     9901 cttgcgcgcg tctccggcca gcaggcttgg gtcggcctcg gccagcgcca cgcactgttc
     9961 gagcagcgag agggcgcggc gggcgtcgcc gtcgctcagc cccgcgatgc gctccagcac
    10021 ttcgggctcc gcgcgccagc cgccggcgcc cagcccgcgt tcggcgtcgc gcagcgcgcg
    10081 ctcgagcagc gtcaccagct cgcccacgtc gagcggtcgc agcgtcagca cgcggcagcg
    10141 cgacaggagc ggggcgatga ccgaaaacga gggattctcc gtcgtcgcgc cgatcagcag
    10201 gatcgttccg ttctcgacgt gcggcagaaa ggcgtcctgc tgcgccttgt tgaaccggtg
    10261 gatctcgtcg atgaacagga tcgtgcggcg gttcttgagc gcgagccggg ccttcgcctg
    10321 gtcgatgacg gccttgacct ccttgatgcc gctgatgacg gcgctcagcg gctggaaagc
    10381 catgtcggtc tggccggcga tgaggcgcgc cagcgtcgtc ttgccgcagc cgggcggccc
    10441 ccacaggatg agggagtgga gcgcgccgcg ggccagcatg cgccgcagcg gcgagtccgg
    10501 cccggcgacc tcggcctggc cgatccagtc gtcgaacgag cgcggccgca tccgctcggc
    10561 cagcggctgg tgcgcggccg ggcgctcgcc gccgccgtcg gcgggttcgg gcggctcggg
    10621 tttgtcgtcg aacagatcca cggccgtatc gtagcagagc ccccgcccgc ccgccagatg
    10681 gcccgcgcgg ccaaagtggc ggggaggagc agcgccgaca atggcgaggc cgcactcctg
    10741 cactcatcgc tagcgtccct gctgtccctg gcggcccctc cctgctagaa taaaccgggt
    10801 tgctgcgaac gcccccggcc cagggaccaa ttcgcgcgga gaatgggcgg aggaggagac
    10861 gatgaatatt tcaccaggca aacggcaact gctggtcgtg ggcgaccgcg tgctgatcaa
    10921 gccgcagacc ggggacgaac ggaccgattc gggcctgatc ctgccgcaga cggtggccga
    10981 ccgggcgcag gtcaactcgg gccgcgtcgt ggcgatcggt ccgggcacgc cgatgccgtt
    11041 cggcgaggag gaccacgacg agccctggcg ccaagtcgag cgccgcccgc gctacatccc
    11101 gatgcaggcc aaggtgggcg acctggcgct ctttctgcgc aaggccaccg tcgagatcaa
    11161 atacgagggc gagacgtatc tgatcgtgcc gcagtcggcg attctggtcc tgctgcgcga
    11221 cgaagacgaa gcgctgttcg cggactgagc cggccggacg ccgcgcgatg gccgagcccg
    11281 cccgcctccc cgcgttgctg ctgtcgggcc cgacgggcat cggcaagtcg gcgctcgccc
    11341 tgcggctggc cgacgagctg ccgctggaga tcgtttcggc cgactcgatg cagatctacc
    11401 gcgggctggc gatcggcacc gcccagccgt cagccgagga acggcgccgc gtgccgacgc
    11461 atctggtcgg cgagctggac ccgaccgagc gcttcgacgc gcggcggttt gtcgagcgcg
    11521 ccgaccagct ccgccgcgag atccacgcgc gcgggcgctt gccggtcttt gtcggcggca
    11581 ccggactgta tctgcgggcg ctgcgctggg gcctgttcga ggggggcggg cgcgatgagg
    11641 cggtccgcgc ggcgctggcg gcggaggccc gccaggccgg ctcggcggcg cttcatgcgc
    11701 ggctggcccg ggtcgatccg gccgcggccg aacggatcgc ccccaacgac gcggtgcgga
    11761 tcgtgcgggc gctcgaggtt cacgtgctga ccgggcggcg gctgagcgag ctgcaacgtc
    11821 aatggacggc gccggcgccg cgcttcccgc atctgctcgt cgtgctgttc gagcggcgcg
    11881 acaggttgcg cgaacggatc gcccggcggg cggacgcgat gctggcggcc ggctggatcg
    11941 acgaggtcgc cgagctgctg gcgcggggga ttccccccga ggcgcactgt ttccgcgcgc
    12001 tgggctaccg cgagatcatc gcgcacctgc gcggcgagct ctcccgcgat gcgctggcgg
    12061 agcggatcgc gacggcgacg gtccagttcg ccaagcgcca gatggtctgg ttccggcgcg
    12121 agcggccggc gatctggctg ccggcgggcg atggcgacgc gggcgaacgg gttgccgacc
    12181 tggcctggct tgcccggcgg ctgctggccg aatgcggcgc ggcgcgataa cagggtgaca
    12241 agttgcgccg gcgatataag ctctaatcag cgtttgcacg ccccgcccgc ggccccccgc
    12301 gcgtggagac ccctgatccg gcccgggaat tcccccagcg acaacaggtt cggcggttcc
    12361 ggcggcccgc ccgcccggcc caatccggcg attgatccgg cgatcgggcg cgcgccggtc
    12421 cgtccggcgc cgcaacttcg gaaagatcaa gggtcgacgg cggcaaaacc cgattgggct
    12481 tgagtttttg cacccgagcc ggtttgatga ttggctcatt ctgcccggtc ggcggcgatg
    12541 cgaacgcgat ggctcgcgcg cccggctctg gcggcgacaa aggaacacag gctctcatgg
    12601 cgaagtcggt tatgaatctg caagacagct acctgaacca ggtccgcaag gataatacgg
    12661 acatcacgat cgttctgttg gacggaaccc ggctcaatgg gcaggtgcgc ggcttcgaca
    12721 atttcacggt gatcctcaat gccgcgggct cgcagcatct gatctacaag cacgcgatca
    12781 gccagatcat ctcgcagcgc cccgtggccg gcaacggcgg ggggcacgag gggggccatg
    12841 gtcgcggcga gcgcggacgg caccgggcgg aaggcgacgc gggcgaacgc ggccggccgc
    12901 gcggcgaagg cgaagggggc ggccgggggc gtcgcggcgg cgcgccgcgc gaaggcgacg
    12961 ccggccatcg cggccagccg ccgcgcgacc gcgagcgcga cgaaaaattc aacacgatcg
    13021 acctgtcgca ggtccaggtc ggcgagaaaa aggaagcctg attgcacggc gcggatcaag
    13081 caccgacatc gccgcaaccc gggccgacgg ccggcgcgcc ggacccggcc gattcgcccg
    13141 ccctccccac tcgttccctt cctcctgagc ttgacctccc atccctcgat gccgcggtga
    13201 accggatccg cggcgcgcgg cggatccagg tcgtcggcca cgtgcggccc gacggcgact
    13261 gcatcggcgc gctgctggcc acgcaccacc tgctggccca gtgggggatc gagcacgcgc
    13321 tggcggcggc ggcgatgccg atctcggcct atgatgacct ggcgggcttc gagcggatcg
    13381 ccgagcagcc ggccggttgg cggcccgacc tggtcgtctt cgtcgacacc gccgacctgg
    13441 cccgcggcct gccgggttgg acgcccggcg cgccgatcgt caacatcgac catcacgccg
    13501 gcaacacgcg cttcggcgaa atcaactgga tcgagccggc gtgcagttcg gtgtgcgaga
    13561 tgctcttctg gctggcgcga cgcgccgccg cgcggatcga ttgcgacctg gccgaggcgc
    13621 tgctgctcgg cctgatgacc gacaccggct cgttccggtt caacaatacg ggggcgacgc
    13681 agttgcgcgt ggcggccgaa ctggtcgagg cgggcgcccg gcccgatggg atcgcccgcc
    13741 tggcctgggg cagccagacg ttcgaggggc tgtcgctgct ggggaccgtg ctcagccgca
    13801 tccggctgct gtgcggcggc cggctgagct ggagcgagct gcgccgcgcc gactatgccc
    13861 gcgtcggcgg cgagcagcat gccccggaga acctggccga tcagcttcgc gcggtgcgcg
    13921 gcgtgcggct cgggctgctg ttccacgagc tgcccgacgg cgcgatgcgg atcaattacc
    13981 gttccgacgg cgcggtcaac gtctcggcgc tggcgaccgc gctgggaggc ggcggccacc
    14041 cggcggcggc gggcgcgtac ctgaccggcg ccgattacga ggccgtccgc gaccgggcga
    14101 tcgccgacgc ccgccaggcg ctcgaagcgg cgctgggcga agcgcccggc gcatcggagc
    14161 cgacgggcgg ctgactacgg atttcggcca cgatccacga ccggcatgat cattgacttc
    14221 catacccacg cgtttcccga cggcctcgcc gagcgggcga tggcgcaact ggcgccggcg
    14281 gcgggcgatg ccttccgccc gcatcacgac ggcacgctcg gtggcctgct ccggctgatg
    14341 gacgcggcgg gcgtcgaccg ggcggtcgtc gcctcgatcg cgaccaaacc ggcgcacgct
    14401 gggccgatcc ttgactggtc gctccggatc gcctccgatc ggattatccc gttcgcctcg
    14461 gtgcatcccg acgacgggga ggcggtccgg cgcgtcgagg cgatcgccgc ggcagggctg
    14521 gcgggcatca agctgcatcc gttctaccag cggttcacgg tcgacgaggc gcggctggac
    14581 ccgatctacg acgcggcgcg ggcgaacggc ctgatcgtcc tgctgcacgc gggctacgac
    14641 gtcgcttttc cccgcgaccc gctggcgacg ccggccgcca cggcgcgcgt cgtcgcgcgc
    14701 caccccgggc tcaaactggt ggcggcgcac ctggggggct ggatggactg ggagcgcgcg
    14761 cgggcggatc tggtcggccg gccgtgctgg ctcgatctgt cctcgtcgct ggaattcctg
    14821 acgccggcgg cggcgcgtga cctgatcgaa cgccacggcg cgcgccgcgt gctgttcgcc
    14881 accgacgcgc cgtggtccga tccggcgcgg acgctcgcgc gcctgcgcgc gctggagctg
    14941 gacccgcgcc aggaggagtg gatcctgtcg gcgaacgcgc gggaattgct ggcggaggat
    15001 gcgcgcgagg cgcaagcggc cccgcccacg cccaatcagg tcccttcatc gtgatcgctg
    15061 gtgggacggt gcgtctcgcc tgagtatttt cagcgtcacg actcaagccc cccgtacttt
    15121 gaggagccca cgaccatcac ccattggctg cgaatcggct ccctcgagtt tcatcccacc
    15181 gccatgcccg tcgagaccgg agtgaaggag tggcgccagt acgttcagca tgggggactc
    15241 ctgagcggcg tgccgacgca attcggcggc gcatcccctg gcctcacgac cacagccgcc
    15301 ctgctgcgcc gctggctgcc cgggccgtgg cggctcgcgc cgctgttccg cccggcgcgc
    15361 gagtgagctt tccactccgc cgccggcgta atgacggaat gggaaatggg gcgacggctc
    15421 cggggtttca cacgctcccc acgtttcctg cgttactcgc cttgcccgcg tcccccgcgt
    15481 tcctgcattt ctcacgttcc gcagcgtccc cagcgtccct ggactccgtt gccttttccc
    15541 gccctttcaa acaagaaggg cagcggctgg tggaccgctg ccctcttcaa ttcccggggt
    15601 ggggcgggct tcgcccgcgc ctcccgcgat gtcatctcaa cgcaccggcc gcgtcaggcg
    15661 gcgaccgaac cgtgcgggtc gatcacgtac ttcttcgcgg cgcccttgtc gaactcgtcg
    15721 tagcccttcg gcgcgtcgtc gagcgagatc agcgtgacgt tgaccgcgtc ggcgatctgg
    15781 atcttgtcgt aaaggatcgc gttcatcagc tggcggttgt acttcatcac cgggcactgg
    15841 ccggtgtgga agtggtgcga cttggcccag cccaggccga tgcggatgcc gagcatgccg
    15901 atcttggcgt tctcgtcgac gccgcccgga tcgccggtga cgtagaggcc cgggatgccg
    15961 atgccgccgc cggcgcgggt gatgtccata acggcgttga gcacggtggc gggctgttcg
    16021 gtgttggcgt tggcgccgtg gccgcgcgcc tcgaagccga cgcagtcaac ggcgtaatcg
    16081 acctcgggtt cgccggtgat ctgctcgacc tgttcggcga gcgaggcctt ctggcgcagg
    16141 tcgatcgtct cgcagccgaa gcggcgggcc tgctccaggc gctcggggat catgtcgccg
    16201 acgatgacgc aggcggcccc cagcaactgg caggaggcgg cgcaggccag gccgaccggc
    16261 ccggcgccgg cgacgtagac gatcgcgccg gggccgaccc cggcggtcac cgcgccgtgg
    16321 tagccggtgg ggaagatgtc cgacaggagc gtcaggtcgc ggatcttcgc catcgcctgg
    16381 tccttgtcgg gaaatttgag caggttgaag tcggcatagg ggaccatgac gtattcggcc
    16441 tggccgccga cccagccgcc catgtcgacg tagccatagg cggcgccggg gcgtgcggga
    16501 ttgacgttga ggcagatgcc ggtcttgcct tccttgcagt tgcggcagcg gccgcaggcg
    16561 atgttgaacg gcaccgagac gatgtcgccc tgcttgatga attcgacatc gcgtccgcac
    16621 tcgatgacat cgccggtaat ctcgtggccg aggacgaggc cgggaggggc ggtggtgcgg
    16681 ccgcggacca tgtgctggtc gctgccgcag atattggtgg tgatgatctt gaggatcacg
    16741 ccgtgttcgc acttgcggtt gccgagggcg agtttggggt aatcgatgct ctggacctcg
    16801 acgtggcgct gtttgagata aacgaccccg cgattgctgg ccatggtagg actctcccgt
    16861 gggaaggtga aggtgccgcg ccgaagaggc ggcacgatgc gggcccggcc atcgggccgg
    16921 acggcaagga gcgaggcgac tcccgggtta aaactggagg attcaaggat gaataaccga
    16981 gttccgcgtc aacaaatcta gcgcggccgc ggggggtgcg caaggctttt gtgcgaaaaa
    17041 ataggccgcc tgacatgccc tcctgaacct gaccccggta tgaacccgaa ggcgacgcaa
    17101 tgcgggtttt tcgagagccg aggacgaggg acgagagccg agggccgagt tcttggtgga
    17161 ggtccgtcgg gggcaaggcc acacaggaga gtggcgttcc caggtttcgg ccggatttcg
    17221 agtgccgagc gccgaactcc tgggtccgat gagggcgatg acgaggccgc gggacgcagc
    17281 ttggagtctg ggacggaatc gggccgtcag gcctcgaact cgatcagccg gatcggattg
    17341 ccctcggggt cctcgaattc ggcgaggcgg aagccggggc cgtattcggg cgggcgcagg
    17401 attgtcacgc cggcggtctc cagctcgcgg caggcgccgt ccaggtcgtc cacggcgatg
    17461 ctgaactgaa cggcgcc
//
LOCUS       DAOPCO010000052        17355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3357_length_17355_cov_5.328092, whole genome shotgun sequence.
ACCESSION   DAOPCO010000052 DAOPCO010000000
VERSION     DAOPCO010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 17355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17355
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3357_length_17355_cov_5.328092"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLS90_05815"
     CDS             complement(<1..664)
                     /locus_tag="PLS90_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01954.1"
                     /translation="MLRRSRMVHSIGGVAAALVIAGLACRGHAQTDPARVQVREGYAI
                     SVAVDELPGARFLALDPDGTLYVSRPGPGDIVALRNADGDGVYEQRTAFVEGHRTVHA
                     MQWVEGWLWFTQTGAVLRARDTDGDGAADETVQVLGDEVLPADGSGHWWRSLLVHDGR
                     IYTSIGDAGNIEDPGDSKRQKIWSYALDGSDERLFASGLRNTEKLVVRPGTDEIWGMD
                     HGS"
     gene            872..1753
                     /gene="prmC"
                     /locus_tag="PLS90_05820"
     CDS             872..1753
                     /gene="prmC"
                     /locus_tag="PLS90_05820"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPK01955.1"
                     /translation="MPHDDSSSLTARRALELGVQRLQAAGIESARLDMSLLLAEALHT
                     DRLGLLTDLDRPLKEAERLRAREYLARRIRREPVAYILGRRDFYGLTFELTRDVLIPR
                     PETEHLVERALAWLADHRGDRPAPLVADVGVGSGAIAVAVARNDTAVRVIGVDSSAAA
                     LAVAGRNAQRHGVADRIELRHGSLLEPVAETLDAILSNPPYVAETERVQLPPEVADWE
                     PAEALFGGRSGLDAIAELIRQAPDRLRPGGLLLIELGAGQADTARMLGGMQLNLECVA
                     VHRDYAGHPRVLEARKR"
     gene            1957..3273
                     /locus_tag="PLS90_05825"
     CDS             1957..3273
                     /locus_tag="PLS90_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK01956.1"
                     /translation="MVSSITSCHIMRQALGIVLGGGRGTRLWPLTKYRAKPAVPLAGK
                     YRLIDIPISNCLNSGLDRIYVFTQFLSSSLNRHIGRTYQLDPFSRGFVNLQVANLSDD
                     NQNWYQGTADAVRRNLKELAQWRTPYVIILPGDTIFRMNLEDLLRFHIDNQAEVTISL
                     HPASADRATGFGIVTLGEDNRIEHMSEKPSADRLPPLAALPAIRQRWQMSDDRPFLGS
                     MGIYVFNFDLLMDLLRNPAMTDFGSDILPYCVSQGRSYGYVFNGYWEDIGTIRAFFES
                     NLDLVKPNPPFQFYLPDAPIYTRMRFLPASQFERARLTRTKIAEGCIIQDAELDTCLV
                     GLRSRVGRGARLRNTILMGADFFESEGNRREYEPVPEGAPAIGIGEDCDVEGAIIDKN
                     ARVGAGCKLLNRDRREHFDDPDERYYIRDGIIIVPKHAILPPGTEI"
     gene            3300..3980
                     /locus_tag="PLS90_05830"
     CDS             3300..3980
                     /locus_tag="PLS90_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01957.1"
                     /translation="MTSRTRRSQEQAPAEPPGPAPIEEAQWQREFLTRRRLRDARIPA
                     RFQNKSLDSFSARDRFRKQLVHGARAYISGFTFAQEYPKGLFMSGQVGCGKSHLAVAI
                     LRGVIEKGYSGLYYNSPDLLRDIRATFSDASPLTEDDLLEEVTTTDLLVFDDVGAENA
                     TGFVLDRFYLIINERYEGCKPLIITTNLDLESLENRLGKRIVSRLLEMCEIFGPFPDE
                     DWRRRNLR"
     gene            3980..4915
                     /locus_tag="PLS90_05835"
     CDS             3980..4915
                     /locus_tag="PLS90_05835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01958.1"
                     /translation="MPTPATDPPPIAFGVLIPNWNGEAFIERCLGSVLAAARHAGRPM
                     ELVVVDDASADQSPRLIAERFPQVRLIRLARNVGFGRAVARGMAELSADWVFLLNNDV
                     ALRADFFSRLIETLHAGLAEGKRILAIGGRTLDWETGAINHGGQRRGRERGLIVQQPF
                     EAEEAAPADFFQAGACLIHRPAFLALGGFADLYHPGYWEDYDLALHGAARGLTCLYEP
                     RAIAWHCGKGSMRRRLGESGLALVLRRNHLLFNWVNLPLRDLLRHLARLGPLCLAGLP
                     RERGSAAPCPWGRAVLAALVRLGPVLRLRRRRARG"
     gene            5580..7028
                     /locus_tag="PLS90_05840"
     CDS             5580..7028
                     /locus_tag="PLS90_05840"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPK01959.1"
                     /translation="MLIGVLIYFSHLRRRIAQLRAIARQAQHEQEAVLTLIDKLGEKI
                     TSKIDLEETLRILTEYIVEATRAESGAIFVLNEAERALQARVVIGPFPPLHETHDYVL
                     TKPKYLTEKIKKDRIQVGEGIIGLVAEEGVPLLITDAEADPRVPRHTTLLSQVHSLIL
                     CPLRVRGQVLGVFVVVNKLGEAIFDSRDLALLQALADQAAVTTDLVKLYDVLAEQQRL
                     ENELRIAHEFQRMLLPSSFPCIENLDFYAMSEAATVVGGDYFDFFEVDEDHLGLVIAD
                     VSGKGIPGALIMAMVRAVLRAEARDNLSPKDVLSRVNERVLHDTKENVFITMTYGILN
                     IRNHVLRFVRAGHEPLLVREAAAEWNGQNGVSQVSPEGIALGLVNDEIFRNTQEVEVA
                     LRPGDVALLYTDGVIEAMDQASQEYGRERLIQMVAGCDGASARDLVARVVEDIHQFTL
                     GIPQHDDITMLALRVRGPGAQEQKLEPTRRTA"
     gene            7025..7423
                     /locus_tag="PLS90_05845"
     CDS             7025..7423
                     /locus_tag="PLS90_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK01960.1"
                     /translation="MSQPASPQTGGQGALKPFALERLYPDGGPPGIVEVRVSGFLDAH
                     TVDVFEKSMEETIAQGYNRVIMNLGALNYISSAGIGALMVLLQQLRRRQGEMVILQPS
                     AKVFKILDLLGFTKIFSIADSPEAARQHLA"
     gene            7566..8063
                     /locus_tag="PLS90_05850"
     CDS             7566..8063
                     /locus_tag="PLS90_05850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK01961.1"
                     /translation="MQHHSARAPQPGGDYHFEVYCPVDTSLLNILRRFVSSVAEAMGF
                     GDEEIDKIEMAVNEACTNVALHAYRDLDRRPDGKPGIELRLSLAPDCLTISIQDRGCG
                     LAAAIAESAITLQDYHHLDRTDYHGLGVLIMKEFMDEVQFSSEPDAGTIVTLRKYIRP
                     GAGQV"
     gene            8090..9676
                     /locus_tag="PLS90_05855"
     CDS             8090..9676
                     /locus_tag="PLS90_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPK01962.1"
                     /translation="MDARPAHAPASSLASRPSARQESSNLMTHMIERVRMINRIHAAL
                     RSSLSLEDIYSIALTALISRHGLGFSRAFLFEVDEVAGELRGLSALGAASRDEHERLH
                     AEIDREEERLAHMVQDLHGLDEAPRGEESLFGQSLRELSSHSFWITTYQKFSAANDMQ
                     ERLRRVRIPWRPRGTTDAAARFGDPRQPAPPRAAAGLLSEVLAGDSAVLVTPDRLAAA
                     TVPEDVKELLPHEGLWSPIRTQRGVRLLLIVDKPFQDEPLDHLDIYHMEWFAGQLALA
                     LDNAEMYTDLETAYHSLRELDQMKSNFLATISHELRTPLTAITGYVQLLLANRVGPVT
                     QGQKEVLERIRSHGELLTGKVNDIIEIAEIDSDNALEVELKPVDPLNVLMAALPRVES
                     RRGQKRISIEPIVTEAIPPVWANPESLERIFFHLLDNAVKFGHQHGHVRIDFTPRGDE
                     LRIRFIDDGIGISAAQLKHIFDAFYQVDNQLTRSYEGLGIGLAIINKQLALTRGRIEV
                     VSEEGKGSIFTVIYPLAQTS"
     gene            9700..10917
                     /locus_tag="PLS90_05860"
     CDS             9700..10917
                     /locus_tag="PLS90_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK01963.1"
                     /translation="MSKRFRVAAARGPRRASAVDPRRRPLNRRHDLFHALRQVSFTVA
                     QGRTLGLVGPNGSGKSTLLKILGGVMQADAGTIRMTGRVGALLELGAGFHPDLSGIDN
                     VYLNGALLGIPGREIDRLLPEIIRFAELERFMDLPVKHFSSGMTARLGFALAAQLAPD
                     ILLMDETFATGDARFQARALGRISEMKGQGRTMILVSHNLDMLLQLADEVLWLEQGAV
                     RAIGPAREILVEYRERGHARLFGSDQMLARLGLEGLFQPLAPPGAAPVRIERVAWAEP
                     AADGGATARVAPGGVLALRIALRHEGDHPARVRLELAWQRRRDGLVLGQTRCEVELEP
                     GGATERTLVFRDWLLEEGHWNLAAAVAPAGEPRPVFYDRLLDAGQVEVQTPAPLELPA
                     AARIPARWEVAEN"
     gene            complement(10935..11789)
                     /gene="larE"
                     /locus_tag="PLS90_05865"
     CDS             complement(10935..11789)
                     /gene="larE"
                     /locus_tag="PLS90_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPK01964.1"
                     /translation="MSSAPAPPLDPPHRDKLQRLLDHFRRMGRVLVAYSGGVDSTLLL
                     KAGTLALGDACLGVLARSETLTDDEFNAALAIARDHGFNLQTIAYSELAIEHYQQNPV
                     NRCYFCKHELYARLTDLAGRLGIPFIAEGSNADDVGDYRPGLQAVAELRVVSPLREAG
                     LTKADVRDLARALGLANWDKPSNPCLSSRIAYGIGIDREKLRQVAEGEKHLRSLGLRQ
                     VRVRHHDQIARIEVAPEEMDRLLERENRQSAWAKLKELGFRYVTVDLAGYRTGSLNEV
                     LPGRDAAP"
     gene            12043..12249
                     /locus_tag="PLS90_05870"
     CDS             12043..12249
                     /locus_tag="PLS90_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011912416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HPK01965.1"
                     /translation="MARWFIVAGVLLIALGLILHYAPWMLRWFGRLPGDIRIETDGVK
                     VYIPIATMIVVSLLLTLLLNLLKR"
     gene            12626..14575
                     /gene="dnaK"
                     /locus_tag="PLS90_05875"
     CDS             12626..14575
                     /gene="dnaK"
                     /locus_tag="PLS90_05875"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK01966.1"
                     /translation="MGNVIGIDLGTTNSVVAFLELNKPQVLPNSEGGKTTPSVVLYRS
                     DEEIVVGELAKRQRLLLPERTIYSVKRFVGCRWDESESRRRGIAYPLCEGPNGTVAIE
                     IDGRRLLPEEVQARTLLKMKQTAEDYLGTTVTQAVITCPAYFNDSQRQATKRAAELAG
                     LEALRIVNEPTAAALAYGLSKRHNERIAVFDFGGGTFDISILEIDEDVIEVKSTCGDT
                     FLGGDAIDQRLTEFILEHIERDLGLDVGGDPQVMARVAEAAEKVKCELSSMPSTIISL
                     PFLGADAGGAKHLNLPLERKQYEEMVAPILERLREPCRQAMADARLSGKDLSSVVLVG
                     GSTRIPAVRRLVEEIFGRAPDHSINPDEAVALGAAIQAGIMSGEIDEILLLDVTPLSL
                     GIELAGGLFSPLIQRNSSIPTTARKKFTTVRDNQQAVTVHVLQGERRIALENRTLARF
                     RLEGISPAPREVPEIEVSFNIDANGILNVAAMDLTTGQLQQVKVESYQPVLDADAQRR
                     IDDAEQNADEDRSFVRKAAIRRRMEEMRMDMEALQRQSDTRPLTEREERRFTERMFRL
                     DVALQNSDWGVIDSAERELKSVFSEIMMLVGRSAGLELDDLIFEADERREKENAAGRA
                     PSEEFKPNGAADPAASPASLDTPGA"
     gene            complement(14619..16463)
                     /locus_tag="PLS90_05880"
     CDS             complement(14619..16463)
                     /locus_tag="PLS90_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPK01967.1"
                     /translation="MSLLVHWQAIYTLVILVLALIALIRNIAPPDAVFLGSVVLYAIA
                     GIISPHEAFSGFANEGVLAVGALFVVAAGLQQTGLLGFIGHHLLGAVRTERVVLARLA
                     ALVLPLSAFLNNTPIVAMLMPVVVDWCRKRSISPSRLLMPLSYLAILGGVCTLIGTST
                     NLVVHGLMVEAARDAADWAARHPGVDPATLATRLRGMSMFEISRLGIPFALVGTAYLF
                     LARPLFPQRKELLEQFGERRREYMAEMVVQPGCRLAGRTVETCGLRNLPGLFLVEINR
                     ADQAITPVGPQDVIQEGDHLVFSGIVSTIVDLERIPGLAPAVDATYDGAGDGRRARQL
                     SEAVISPTSPLIGVKVRDSNFRALYDAAIVAIHRGGARLNTKIGEVALRPGDTLLLQT
                     GADFARRHRNHPDFYLVSDVEDSAPPRHARMPVAIVLFLVLIALMSSGVIPISVAALL
                     VAGLMVLFRCMSAADARKSVEWQVLVTIAASFGAGAALKNSGAAHFLAQSLVQLTAGA
                     GPVVGLAATFFVTTVLTAVITNNAAAVLMFPIVLDAALQFGVNPRGWLFALALGASTC
                     FISPIGYQTNMMVYGPGGYRFTDFLRVGGPMQLALLAVVMALTPWLWP"
     gene            complement(16561..>17355)
                     /locus_tag="PLS90_05885"
     CDS             complement(16561..>17355)
                     /locus_tag="PLS90_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HPK01968.1"
                     /translation="RKATREAKEHTSWINPNDDYDEAVQQFVAEILRAGRRNFFLTHL
                     EKFQRPLAALGAVNALSQTLLKLTSPGVPDIFQGDELWNFCLVDPDNRRPVDFVLRRH
                     LLADLRRRIAGAEFNLAPLADELLGAWRDGRIKLYVTHRLLQLRRQAPALFRDGEYLP
                     LTVHGDRADHVCAFLRRREQDELLVIVPRLLVGLCGFPADADPPWPVGESIWRDTWID
                     LPERPAAPWKNLFTDETPPLSPDHALKLADALSRFPVAAFVRSPQT"
BASE COUNT         2927 a         5845 c         5894 g         2689 t
ORIGIN      
        1 cgctgccgtg gtccatcccc cagatctcgt cggtccccgg ccggaccacc agcttctcgg
       61 tattgcgcag gcccgaggcg aacagccgtt catcgctccc gtccagcgca taggaccaga
      121 tcttctgccg cttgctgtcg cccggatcct cgatgttgcc cgcgtcgccg atgctcgtgt
      181 agatgcgccc gtcgtgaacc agcagcgagc gccaccagtg cccgctcccg tcggccggca
      241 gcacttcgtc gcccagcacc tgcacggttt catccgccgc gccgtcgccg tcggtgtcgc
      301 gggcgcgcag caccgccccc gtttgcgtaa accacagcca gccctccacc cactgcatcg
      361 cgtgaacggt gcggtgcccc tccacgaatg ccgttcgctg ctcatacacc ccgtcgccgt
      421 ccgcattgcg caacgccacg atgtcccccg gaccgggccg gctgacgtag agcgtcccgt
      481 ccggatcgag cgcaaggaac cgcgcgccgg gaagctcgtc gaccgccacg ctgatcgcgt
      541 atccctcgcg cacctgcacc cgcgcgggat cggtttgcgc gtggccccgg caggccagac
      601 cggcgatcac gagggcggcc gcgacgcccc cgattgagtg caccatccgg gatcggcgga
      661 gcatggcgga cctcctgtca cagcggaagc ggattgcaat cccgatcatt ctacccgctc
      721 ccggcggaaa aggcaaaaac tcaaccctgt cgaaccccgc cccgacggca tcacccaccc
      781 tcgcgcgccg cgccctgccg ctttacctca cccgccgttg caggcatcat ggcccgggcg
      841 cggttctcga ctcggaagga acggttgcgg catgccacac gacgacagct ccagcttgac
      901 ggcccggcgg gcgctcgagc tcggcgtcca acgcctccag gccgccggca tcgaatccgc
      961 ccgcctcgat atgagcctgc tcctggccga ggccctccac accgaccggc tcggcctgct
     1021 gaccgacctc gaccggccgc tcaaggaagc cgaacggctc cgcgcgcgcg aatacctcgc
     1081 gcgccgcatc agacgcgaac cggtcgccta catcctcggc cggcgcgact tctatggcct
     1141 gacgttcgag ctgacgcgcg acgtcctcat cccccgaccc gagaccgagc atctcgtcga
     1201 acgcgccctg gcctggctcg cggaccatcg cggcgaccgg cccgcgccgc tcgtcgccga
     1261 tgtcggcgtc ggctccgggg caattgccgt ggccgtagcc agaaacgaca ccgccgtccg
     1321 cgtgatcggc gtcgattcct ccgcggcggc gctggccgtc gccggccgca acgcccaacg
     1381 ccacggcgtc gccgaccgga ttgaactgcg ccacggctcg ctgctcgaac ccgtcgccga
     1441 gactctcgac gccatcctga gcaacccccc ctacgtcgcg gaaacggaac gggtccagtt
     1501 gccccccgag gtcgccgact gggaaccggc cgaggccctc ttcggcggtc gcagcggact
     1561 ggatgcgatc gccgaactga ttcgtcaggc gcccgatcgg ttgcgtcccg gcggcttgct
     1621 cctgatcgag ctcggcgccg gtcaggccga caccgcgcgg atgctcggcg gcatgcagct
     1681 caatctggaa tgcgtcgccg tccatcgcga ctacgccggc cacccccgcg tcctcgaagc
     1741 aagaaaacgc tgacgccccc ctcccctccc ccaaaattcg gctcccggct ctcgtccccc
     1801 gtcctcggct ctcgaaaacc cgccattccc tttcgtctca atcgtaatcc cccgcgactt
     1861 gcacgccgtc tccgcctcag gcagactagg gggctgtcgg ccaacccgcc ctctggcggt
     1921 ccttccggcg ccttttcgtt tccgcgggta tctgtcatgg tctccagcat tacctcgtgc
     1981 cacatcatgc gccaggccct gggcatcgtc ctcggcggcg gacgcggcac gcgcctctgg
     2041 ccgctcacca aataccgcgc caagcccgcc gtccccctcg ccggcaagta tcgcctgatc
     2101 gacatcccca tcagcaactg cctgaactcc ggcctcgacc ggatctacgt cttcacccag
     2161 ttcctctcca gttccctcaa ccgccacatc ggccgcacct atcagctcga ccccttcagc
     2221 cgcgggttcg tcaacctcca ggtggccaac ctcagcgacg acaaccagaa ctggtatcag
     2281 ggaaccgccg acgccgtccg ccgcaacctg aaggaactgg cccagtggcg caccccgtat
     2341 gtcatcatcc tgccgggcga cacaatcttc cgcatgaacc tggaggatct gctgcgcttc
     2401 cacatcgaca accaggccga ggtgacgatc tcgctccacc ccgcctcggc cgaccgcgcc
     2461 accggcttcg gcatcgtcac gctcggcgag gacaaccgga tcgaacacat gagcgaaaaa
     2521 ccctccgccg accggctgcc gccgctggcc gccctcccgg cgatccgcca acgctggcag
     2581 atgagcgacg accggccgtt cctcggctcg atgggcatct acgtcttcaa tttcgacctc
     2641 ctcatggatc tgctgcgcaa ccccgccatg accgatttcg gcagcgacat cctcccctac
     2701 tgcgtcagcc aggggcgcag ctacggttac gtcttcaacg gctactggga ggacatcgga
     2761 accatccgcg cgttctttga atccaacctc gacctggtca agcccaatcc ccccttccag
     2821 ttttatctcc ccgacgcgcc gatctacacg cgcatgcgct tcctgcccgc cagccagttc
     2881 gagcgcgcgc gcctgacgcg caccaagatc gccgagggct gcatcatcca ggatgccgaa
     2941 ctcgacacct gcctcgtggg tctgcgcagc cgcgtcggcc gcggggcgcg cctgcgcaat
     3001 accatcctca tgggcgccga tttcttcgag agcgagggca accgccgcga atacgagccc
     3061 gtccccgaag gcgcaccggc catcggcatc ggcgaggact gcgacgtcga gggggcgatc
     3121 atcgacaaga atgcccgcgt cggcgccggc tgcaagttgc tcaaccgcga ccgccgcgag
     3181 catttcgacg acccggatga gcgctactac atccgcgacg gcatcatcat cgtccccaag
     3241 cacgccattc tgccgccggg caccgaaatc tagcgccgcg cgaaggagct ttcagcgtga
     3301 tgaccagccg cacccgacgc agccaggagc aggcccccgc cgaaccgccc ggccccgcac
     3361 ccatcgagga ggcccagtgg cagcgcgaat tccttacccg ccgccgcctg cgcgacgcgc
     3421 gcatccccgc ccgcttccag aataagtcgc tcgactcctt ctccgcccgc gaccgctttc
     3481 gcaagcagct cgtccacggc gccagggcct atatctccgg tttcaccttc gcgcaggaat
     3541 accccaaagg gttgttcatg tcgggccagg tcggctgcgg caagagccac ctggccgtcg
     3601 ccatcctgcg cggcgtcatc gagaagggct actccggcct ctactacaac tcccccgacc
     3661 tgctgcgcga catccgcgcc acgttcagcg acgccagccc gctcaccgag gacgacctgc
     3721 tggaggaggt cacgacgacc gacctgctcg tcttcgacga cgtcggcgcc gaaaacgcca
     3781 ccggcttcgt cctcgaccgc ttctacctga tcatcaacga gcgctacgag ggctgcaaac
     3841 cgctgatcat caccacgaac ctggatctcg aatcgctcga gaaccgcctc ggcaaacgca
     3901 tcgtcagccg cctgctggaa atgtgcgaga tcttcggccc cttccccgac gaagactggc
     3961 ggcggcggaa tctgcgctga tgcccactcc ggctacggac ccgcccccga tcgccttcgg
     4021 cgtcctgatc ccgaactgga acggcgaggc cttcatcgag cgctgcctgg ggtcggtcct
     4081 cgcggcggcg cggcatgccg gccgcccgat ggaactcgtc gtcgtcgatg acgccagcgc
     4141 ggaccagtcg ccccgcctga tcgccgagcg ctttccccag gtccgcctga tccggctggc
     4201 gcgcaatgtc ggcttcggcc gcgccgtcgc gcgcggcatg gcggagctct cggccgactg
     4261 ggtcttcctg ttgaacaacg acgtcgccct gcgcgccgat ttcttcagcc ggttgatcga
     4321 aaccctccac gccggcctcg ccgagggcaa gcggatcctc gccatcggcg ggcggacgct
     4381 cgattgggaa accggcgcga tcaatcacgg cggccagcgc cgcggccggg agcgcgggct
     4441 catcgttcag cagccgttcg aggccgagga agccgccccc gccgatttct tccaggccgg
     4501 cgcctgcctg atccatcgcc ccgcgttcct cgccctgggc ggattcgccg acctctatca
     4561 tcccggttac tgggaagact acgacctggc gctccacggc gcggcgcgcg gcctgacctg
     4621 tctctacgag ccgcgcgcca tcgcctggca ctgcggcaag ggctccatgc gccggcggct
     4681 cggtgagtcg ggcctcgccc tcgtcctccg ccgcaaccat ctcctcttca actgggtgaa
     4741 cctgccgctg cgcgatctgc tccggcatct ggcgcggctg ggcccgctgt gcctcgccgg
     4801 cctcccgcgc gagcgcggct cggccgcccc ctgcccgtgg ggccgcgccg tcctggctgc
     4861 cctcgtccgc ctgggccccg tcctccggct ccgccgccgc cgcgcccgcg gctgatccca
     4921 ggccgtccct cccccccgct ccgccaccgg agtttctcca ggaatcgtct cgtcctcgcc
     4981 cacaccggtc ctctccgaaa attcggctct cggctctcga ccctcgtcct cggctctcga
     5041 aaaccggctt tttcgggacg cagctcaaca cgattcgcac cgcctgccat ttgttggcgc
     5101 ttgccttgag cctgatgaac cggcggctca caacgaggga tgaaaatggg agtgaacggc
     5161 cgatttgaac gatccaggcc ttgccgcatc cgtcagccgg gcagtcccgc ggggacggca
     5221 gaaccgatcc aatcgatccc cggaataagg gacagttttt cgagtgcacg acgcttccgc
     5281 gtcaccctga aggatgaaat ggcctctacc cgttctttag tccttgatgt cccttctgaa
     5341 acactaattt tccaatgaga tccgatcggc ggaccaactc tcgaacaagg tcaagatccg
     5401 cctgtgtccc cgcgtctccg cgtccgggct gatgtcagtt gaccccaccc acggcctgtt
     5461 ccatgatgaa ttgacgtgga gcgcgcgtgc gccgcgtgga atcggctttg gcgcagcatc
     5521 gaacgattcg ccggggtggt gagctgttga gtcctgcctg gaccgttatc tcgattttgg
     5581 tgctcatcgg ggtcctgatt tacttcagtc acctgcggcg ccgcatcgcc cagttgcgcg
     5641 ccatcgcccg ccaggcccaa cacgaacagg aggctgtcct caccctgatc gacaagctcg
     5701 gcgaaaaaat caccagtaag atcgacctcg aagagaccct gcgcatcctg accgagtata
     5761 tcgtcgaggc gactcgcgcc gaatcgggcg ccatcttcgt cctcaacgaa gccgagcgcg
     5821 ccctgcaggc ccgcgtcgtc atcggaccct ttcccccctt gcatgaaacc cacgattatg
     5881 ttttaacgaa gccgaagtac ttgacagaga agatcaagaa ggaccgcatt caggtcggcg
     5941 agggcatcat cggcctcgtc gccgaagagg gagtcccctt gctgatcacc gacgccgagg
     6001 ccgacccgcg tgtcccccgg cacacgacgc tcctctcgca ggtccactcg ctgatcctct
     6061 gcccgctgcg cgtccgcggc caggtcctcg gcgtcttcgt cgtcgtcaac aagctgggcg
     6121 aggcgatctt cgattcgcgc gacctcgccc tgttgcaggc gctggccgac caggccgcgg
     6181 tcacgaccga tctggtcaag ctttacgacg tgctggccga gcagcagcgg ctggaaaacg
     6241 aactgcgcat cgcccacgag ttccagcgca tgctgctccc ctcgtcgttc ccgtgcatcg
     6301 aaaacctgga cttctacgcg atgagcgaag ccgccaccgt cgtcggcggc gattacttcg
     6361 acttcttcga agtggacgag gatcacctcg gcctggtcat cgccgacgtc tcgggcaagg
     6421 gaatccccgg cgcgctgatc atggcgatgg tccgcgcggt cctgcgcgcc gaggcccgcg
     6481 acaacctctc ccccaaggac gtcctcagcc gcgtcaacga acgcgtgctc cacgatacga
     6541 aggaaaacgt ctttatcacg atgacttatg gtatcctgaa catacgcaac cacgtgctgc
     6601 ggttcgtgcg ggcgggccat gaaccgctgc tcgtgcgcga ggcggccgcc gaatggaacg
     6661 gccagaacgg cgtcagccag gtctcccccg agggcatcgc cctgggcctg gtcaacgacg
     6721 agatctttcg caatacccag gaggtggagg ttgcgctgcg tccgggcgat gtcgccctgc
     6781 tctataccga cggcgtcatc gaggcgatgg atcaggccag ccaggaatac ggccgcgagc
     6841 gcctgatcca gatggtcgcc ggttgcgacg gcgccagcgc gcgcgatctg gtggcgcgcg
     6901 tcgtcgagga cattcatcaa ttcaccctgg gcatccccca gcatgacgac atcacgatgc
     6961 tcgccttgcg cgtgcgcggc cccggcgcgc aggaacaaaa gctcgaaccg acacggagaa
     7021 ccgcatgagc cagcccgctt caccccagac cggcggccag ggggccttga aacccttcgc
     7081 gttggagcgg ctctatcccg acggcggtcc ccccggcatc gtcgaggtgc gcgtctccgg
     7141 gttcctcgac gcccacaccg tggacgtctt cgaaaaatcg atggaagaga cgatcgcgca
     7201 gggctacaac cgcgtcatca tgaatctggg cgcgctcaac tatatctcgt cggccggcat
     7261 cggcgcgctg atggtcctgc tccagcagct tcgccgccgg cagggcgaga tggtcatcct
     7321 gcagccctcg gccaaggtct tcaagatcct cgacctgctg gggttcacga agatcttcag
     7381 catcgccgac agccccgagg ccgcgcgtca gcacctggcc tgacgccccc gggcgccgcg
     7441 cgaccggcgg cgcgagcgat ttcgggctgt cgcagtcatc gtcgtgcagc aagcgggggc
     7501 ggaggggctc atcaccgctt gctgactgga tccagaccgg cgcggcgcgg tccggaggca
     7561 cacccatgca gcaccacagc gcgcgagcgc cgcagccggg cggcgactat cacttcgagg
     7621 tctactgccc cgtcgatacc tcgttgctca acatcctgcg ccgcttcgtc tccagcgtcg
     7681 ccgaggcgat gggcttcggc gacgaggaga tcgacaagat cgaaatggcc gtcaacgagg
     7741 cctgcaccaa cgtcgctctg cacgcctacc gcgacctcga ccgccggccc gacggcaagc
     7801 cggggatcga actccgcctc tcgctcgccc ccgactgcct cacgatttcg attcaggacc
     7861 gcggctgcgg cctcgccgcc gccatcgccg aatcggccat caccctccag gattatcacc
     7921 acctcgaccg caccgactat cacggcctcg gggtgcttat catgaaggaa ttcatggatg
     7981 aggtgcagtt cagcagcgag ccggacgccg gcacgatcgt cacgctgagg aagtacattc
     8041 gccccggcgc cggccaggtc tgacgcggcg accggcgatg aagcaaccga tggatgctcg
     8101 acccgctcac gcaccggcca gctcgctggc cagccggccc tccgcccggc aggaatcatc
     8161 caacctgatg acgcacatga tcgaacgggt ccggatgatc aaccggatcc acgccgccct
     8221 gcgctccagc ctcagtctgg aggatatcta ctccatcgcc ctcacggcgc tgatctcgcg
     8281 ccatggcctc ggcttctcgc gcgccttcct cttcgaggtg gacgaggtcg cgggcgaatt
     8341 gcgcggcctc tcggcgctcg gcgccgcgtc gcgcgacgag cacgagcgcc tccacgccga
     8401 aatcgatcgc gaggaggaac gcctggccca catggtccag gacctgcacg gcttggacga
     8461 agcgccccgc ggcgaggaat cgcttttcgg ccagagcctg cgcgagctgt cgagccattc
     8521 cttctggatc acgacctatc agaaattttc cgccgccaac gacatgcagg agcgcctgcg
     8581 ccgcgtgcgc atcccctggc gcccgcgcgg caccaccgac gccgccgccc gcttcggcga
     8641 tccgcgccaa ccggcgccgc cgcgcgccgc cgccggcctg ctcagcgaag tgctcgccgg
     8701 tgattccgcc gtgctcgtca ccccggatcg gctcgccgcc gcgaccgtcc ccgaagacgt
     8761 caaggaactt ctcccgcacg agggcctttg gtcgcccatc cgtacgcaac gcggcgtccg
     8821 gctgctgctc atcgtcgaca aacccttcca ggatgaaccg ctcgatcacc tcgatatcta
     8881 tcacatggaa tggttcgccg gacaactcgc gcttgccctg gacaacgccg agatgtacac
     8941 cgacctggag acggcctacc acagcctgcg cgagctcgac cagatgaaga gcaacttcct
     9001 ggcgacgatc agccacgagt tgcgcacgcc cctgacggcg atcaccggct acgtccagct
     9061 cctgctcgcc aaccgggtcg gacccgtcac gcaggggcag aaagaggtcc tcgaacgtat
     9121 ccgctcgcac ggcgaactgt tgaccggcaa ggtcaacgac atcatcgaga tcgccgagat
     9181 cgactcggac aacgccctcg aggtcgagct caagccggtc gacccgctca acgtgttgat
     9241 ggcggcgctg ccccgggtcg agtcgcgccg cggccagaag cgcatctcga tcgaaccaat
     9301 cgtgaccgag gccatccccc ccgtctgggc caatccggag tcgctcgaac ggatcttctt
     9361 ccacctgctc gataacgcgg tcaagttcgg ccaccagcac ggtcacgtgc gcatcgattt
     9421 caccccgcgg ggcgatgagc tgcgcatccg cttcatcgac gacggcatcg gtatctccgc
     9481 cgcgcagctc aagcatatct tcgacgcgtt ttatcaggtc gataatcaac tgacccgcag
     9541 ctacgagggg ttgggcatcg gcctggccat catcaataaa cagcttgcgc tgactcgcgg
     9601 ccgcatcgag gtcgtaagcg aggaaggcaa aggctccatc ttcacggtca tctatccgct
     9661 cgcccagacg tcgtgacgcc ggccattgaa atcaccggat tgagcaagcg gtttcgcgtc
     9721 gccgccgcgc gcggcccccg ccgggcctcc gccgtcgatc cgcgccgccg gccgctcaac
     9781 cgccgccatg acctgtttca tgccctgcgc caggtcagct tcaccgtcgc gcaaggccgt
     9841 acgctcggcc tggtcggccc caacggctcc ggaaaatcga ccttgctcaa gatcctcggc
     9901 ggcgtcatgc aggccgacgc cggaacgatc cgcatgaccg gccgggtcgg cgcgctgctg
     9961 gaactgggcg ccggtttcca ccccgacctg tcgggcatcg acaacgtcta cctcaacggc
    10021 gcgctgctcg gcatccccgg ccgcgagatc gaccggctgc tgcccgagat catccggttc
    10081 gccgagctgg agcgcttcat ggacctgccg gtgaagcact tcagttcggg catgaccgcc
    10141 cggctcggct tcgctctggc ggcccagctc gcccccgaca tcctcctgat ggacgagacg
    10201 ttcgccaccg gcgatgcccg gtttcaggcg cgcgcgctcg gacgcatcag tgagatgaag
    10261 ggccagggcc gcacgatgat cctcgtcagc cacaacctgg acatgctgct gcaactggcc
    10321 gacgaagtcc tctggctcga gcagggcgcg gtgcgcgcca tcggcccggc gcgcgagatt
    10381 ctggtcgagt accgcgagcg gggccatgcg cgcctgttcg gctcggacca gatgctggcg
    10441 cggctcgggc tggaggggct gtttcaaccg ctggccccgc caggcgcggc gccggtgcgc
    10501 atcgagcggg tcgcctgggc ggagcccgcc gcggacggcg gcgcgacggc gcgcgtggcg
    10561 cccggcggcg tccttgcgct gcgcatcgct ttgcgccacg agggcgacca ccccgcgcgc
    10621 gtccggctgg agctggcctg gcagcgccgg cgcgacggcc tggtgctcgg ccagacgcgc
    10681 tgcgaggtcg agctggagcc cggcggtgcg accgaacgca cgctcgtgtt ccgcgactgg
    10741 ctgctggagg aagggcattg gaatctggcg gcggcggtcg cgccggccgg tgagccgcga
    10801 cccgtctttt atgatcgtct gctggacgcc ggccaggtcg aggtgcagac gccggcgccg
    10861 ctggaactgc ccgccgccgc gcgcatcccc gcgcgctggg aggtggcgga aaactgaagc
    10921 gcgccgccgg acggtcaggg ggcggcgtcg cggccgggga ggacttcgtt caggctgccg
    10981 gtgcggtagc cggccaggtc gaccgtgacg tagcggaagc ccagttcctt cagtttggcc
    11041 caggccgact ggcggttctc ccgctcgagg aggcggtcca tctcctccgg cgcgacctcg
    11101 atgcgggcga tctggtcgtg gtggcgcacg cggacctgac gcagtccgag cgagcgcagg
    11161 tgcttttcgc cctcggccac ctggcgcagc ttttcccggt cgatgccgat gccgtaggcg
    11221 atccgcgacg aaaggcacgg gttggagggt ttgtcccagt tggcgaggcc gagcgcgcgg
    11281 gccaggtcgc ggacgtcggc cttggtcagg ccggcctcgc gcagcgggct gaccacgcgc
    11341 agttcggcga cggcctgcag tccggggcgg tagtcgccca cgtcgtcggc gttggagcct
    11401 tcggcgatga acgggatgcc cagccggccc gccaggtcgg tcagccgcgc gtagagctcg
    11461 tgcttgcaga agtagcagcg gttgaccggg ttctgctggt aatgctcgat ggccagttcg
    11521 ctgtatgcga tggtctgcag gttgaagccg tggtcgcggg cgatggcaag cgccgcgttg
    11581 aattcgtcgt cggtcagcgt ttcggagcgg gccagcacgc cgaggcaggc gtcgcccagc
    11641 gccagcgttc cggccttgag cagcagcgtg gaatcgacgc cgcccgaata ggcgacgagc
    11701 acgcggccca tccggcggaa atggtcgagc agccgttgca gcttgtcgcg gtgcggcggg
    11761 tcgagcggcg gggcgggggc gctggacatc cggagggaac cttggggaaa ttggggagtt
    11821 gcgcgagcgg cgcgggggcg gctgatggaa agacgctccc gcggcggccg cccacgatgg
    11881 tattttacag gttgcgcggc ccgatgggaa cctggaaagc ccgcgcgcgg cggggacggg
    11941 caggttttgg gatgacgggt catgatgcgg gagacagatt gaccggtttt cgagagccga
    12001 ggacgaggga cgagagacga ttatttcggg atggtgaatg ccatggcgcg ctggttcatc
    12061 gttgcggggg tgctgctgat cgcgctcggc ctgatcctgc actacgcccc ctggatgctg
    12121 cgctggttcg gtcggctgcc gggcgatatc cggatcgaaa ccgacggcgt caaggtatac
    12181 atcccgatcg cgacgatgat cgtcgtcagc ctgttgctga cgctgctgct taatctgctg
    12241 aaacgctgat tcgggaattc gccggcaacg ccaagaggcc gaatccgggt ggaattcggc
    12301 gagggagagg tgtgggcggg cgacgggagg gaagaccggt gaagggaggg gggcgggggg
    12361 cggatgccag tcggccaggc cggcggggag ggtggagggg gatgagcggg ccggcggttc
    12421 ggagcgaggg gtgaagatgg cgggttttcg agagccgagg acgagggacg agagccgaga
    12481 gccgaattgt gggagaaggc cgttgggggt gaggacgggg gcttaaccgg ggggggaaag
    12541 tgtccgatga gaacaggaag aagcgggtcc ctggcgcccg acggcggcca ggggcgaacc
    12601 atcccccacg gcgtaagcgg acaacatggg caacgtgatc gggatcgacc tggggacgac
    12661 caattcggtc gtcgcctttc tggagctgaa caagccgcag gtgctgccca attcggaggg
    12721 gggcaagacg acgccgtcgg tggtgctgta ccggtcggac gaggagatcg tggtgggaga
    12781 gctggccaag cgccagcggc tgctgctgcc ggagcggacg atctattcgg tcaagcggtt
    12841 cgtggggtgc cggtgggacg aatccgagtc gcgtcggcgg gggatcgcct acccgctgtg
    12901 cgagggtccc aacgggacgg tggcgatcga gatcgacggc cggcggctgc tgccggagga
    12961 ggtccaggcg cggacgctgc tcaagatgaa gcagacggcg gaggattacc tggggacgac
    13021 agtgacgcag gcggtgatca cctgcccggc gtatttcaac gacagccagc ggcaggcgac
    13081 gaagcgggcg gcggagctgg cggggctgga ggcgctgcgg atcgtgaacg agccgacggc
    13141 ggcggcgctg gcctacggct tgagcaagcg gcacaacgag cggatcgcgg tgttcgattt
    13201 cggcggcggg acgttcgaca tctcgatcct cgagatcgat gaggacgtga tcgaggtcaa
    13261 gtcgacgtgc ggcgatacgt ttctgggcgg cgacgcgatc gaccagcggc tgacggagtt
    13321 cattctggag catatcgagc gcgacctggg gctggacgtg gggggcgatc cgcaggtgat
    13381 ggcgcgggtg gcggaggcgg ccgagaaggt caagtgcgag ctgtcgtcga tgccgtcgac
    13441 gatcatttcg ctgccgttcc tgggggcgga cgccggcggg gcaaagcacc tgaacctgcc
    13501 gctggagcgc aagcaatatg aggagatggt ggcgccgatc ctggagcggc tgcgcgagcc
    13561 gtgccgccag gcgatggccg acgcgcggct gagcgggaag gatctgagca gcgtcgtgct
    13621 ggtggggggg tcgacgcgga tcccggcggt gcggcggctg gtggaggaga tattcgggcg
    13681 cgcgccggat cattcgatca acccggacga ggcggtcgcg ctgggggcgg cgatccaggc
    13741 cgggatcatg agcggcgaga tcgacgagat tctgctgctc gacgtgacgc cgctgtcgct
    13801 ggggatcgag ctggcggggg ggctgttctc gccgctgatt cagcgcaaca gcagcatccc
    13861 gacgacggcg cgcaagaagt tcaccacggt gcgcgacaac cagcaggcgg tgacggtgca
    13921 cgtgctgcag ggggagcggc ggatcgcgct ggagaaccgg acgctggcgc ggttccggct
    13981 ggagggaatc agcccggcgc cgcgcgaggt gccggagatc gaggtcagtt tcaacatcga
    14041 cgccaacggg atcctgaacg tggcggcgat ggacctgacg accgggcagc tccagcaggt
    14101 gaaggtggag tcgtatcagc cggtgctgga cgcggacgcg cagcggcgga tcgacgacgc
    14161 cgaacagaat gcggacgagg accgttcgtt cgtgcgcaag gcggcgatcc ggcggcggat
    14221 ggaagagatg cggatggaca tggaggcgct gcagcgccag tcggacacgc ggccgctgac
    14281 cgagcgcgag gagcggcggt tcaccgaacg gatgttccgg ctggatgtgg cgctgcagaa
    14341 cagcgactgg ggggtgatcg attcggccga gcgcgaactg aagtcggtct tctcggagat
    14401 catgatgctg gtggggcgtt cggccgggct cgagctggac gacctgatat tcgaagccga
    14461 cgaacgccgg gaaaaggaaa acgcggcggg gcgggcgccg agcgaggagt tcaagcccaa
    14521 cggcgcggcg gacccggccg ccagccccgc ctcgctcgac acgccggggg cgtgagggaa
    14581 tcgagagcga tggcgatggc gagggcgagg gcgattatct agggccagag ccagggggtg
    14641 agcgccatca ccacggcgag cagggcgagc tgcatcgggc cgcccacacg caggaaatcc
    14701 gtaaaccgat aaccgcccgg gccatagacc atcatgttgg tctggtaacc gatgggtgag
    14761 ataaagcagg tggaggcgcc gagggcgagg gcgaagagcc agccgcgcgg attgacgccg
    14821 aattgcaggg cggcgtcgag gacaatcggg aacatcagga cggccgcggc gttgttggtg
    14881 atgacggcgg tcaggacggt ggtgacgaag aacgtcgcgg cgaggccgac gaccggtccg
    14941 gcgccggcgg tcagttgcac cagcgactgg gcgaggaaat gcgccgcgcc ggagtttttc
    15001 aaggcggcgc cggcgccgaa cgaggcggcg atggtgacca gcacctgcca ctcgacgctc
    15061 ttgcgggcgt cggcggcgct catgcagcgg aacaggacca tcaagccggc gacgagcagg
    15121 gcggcgacgg aaatcggaat cacgccgctg gacatgaggg cgatcaggac gaggaacagc
    15181 acgattgcga cgggcatgcg cgcgtgacgc ggcggggcgg agtcctcgac gtcgctgacc
    15241 aggtagaagt cgggatggtt gcgatggcgg cgggcgaaat cggcgccggt ctggaggagg
    15301 agcgtatcgc cggggcggag ggcgacctcg ccgatcttgg tgttgaggcg cgcgccgccg
    15361 cggtggatcg cgacgatcgc cgcgtcgtag agcgcgcgga agttgctgtc gcggactttg
    15421 acgccgatca ggggggaggt gggcgagatg acggcctcgg agagctggcg ggcgcggcgg
    15481 ccgtcgccgg cgccgtcgta ggtggcgtcg acggccgggg cgagtccggg aatccgttcc
    15541 agatcgacga tggtcgagac gatgccggaa aagacgagat ggtccccctc ctgaatgacg
    15601 tcctggggcc cgacgggcgt gatcgcctgg tcagcgcgat tgatctcgac gaggaagagg
    15661 ccgggcaggt tgcgcaggcc gcaggtttcg acggtgcggc cggcgaggcg gcagccgggc
    15721 tggacaacca tttcggccat gtattcgcgg cggcgctcgc cgaactgctc gagcagctcc
    15781 ttgcgctggg ggaacagggg ccgggcaagg aagaggtagg ccgtgccgac gagggcgaac
    15841 gggatgccga gacgtgagat ctcgaacatc gacatgccgc gcaggcgggt ggcgagcgtg
    15901 gccggatcga cgccggggtg gcgcgccgcc cagtcggcgg cgtcgcgggc cgcctcgacc
    15961 atcaggccat ggacgaccag gttggtgctg gtgccgatca gggtgcagac gccgcccaga
    16021 atggccaggt aggacagggg catcaggagc cgggagggcg agatcgagcg cttgcggcac
    16081 cagtcgacaa cgacgggcat gagcatcgcg acgatggggg tgttgttgag gaaggccgag
    16141 agcgggagga cgagggcggc gaggcgggcc aggacgacgc gctcggtgcg caccgcgcca
    16201 agcagatgat ggccgatgaa gccgaggagg ccggtctgct ggaggcccgc cgcgacaacg
    16261 aagagggcgc cgacggcgag gacgccctcg ttggcgaagc cgctgaaggc ttcgtggggc
    16321 gagatgatgc cggcgatggc gtagaggacg accgagccga ggaaaacggc gtcggggggc
    16381 gcgatattac gaatgagggc aatcagcgcg aggacgagga tgacgagggt gtaaatggct
    16441 tgccagtgga ccagcagact cacggacgcg atactccgcc gccgggatat gagtggatga
    16501 tacccgccgg gaggaagcgg gggcaacgaa tatcgtaatc ccgatcggaa tagtcgacaa
    16561 tcaagtttgg ggggatcgaa cgaaggcggc gacggggaac cgggacaatg cgtcggccag
    16621 tttcagcgcg tggtcggggc tcaagggggg cgtctcatcg gtgaaaagat tcttccacgg
    16681 cgcagcgggt cgctccggca ggtcgatcca ggtgtcgcgc cagatggatt cgcccacggg
    16741 ccaagggggg tcggcgtcgg cggggaagcc gcacaggccg acgaggaggc gcgggacgat
    16801 caccagcagt tcatcctgct cgcgccggcg gagaaaggcg cagacatggt cggcgcgatc
    16861 gccgtggacg gtgagcggca gatactcgcc gtcgcggaag agggccgggg cctggcggcg
    16921 aagctgaagg aggcgatggg tgacgtagag cttgatgcgg ccgtcgcgcc aggcgccgag
    16981 gagttcatcg gcgagcgggg cgaggttgaa ctcggcgccg gcgatgcggc ggcggagatc
    17041 ggcgagcaga tgccggcgca gaacgaaatc gacggggcgg cggttgtccg ggtcgacgag
    17101 gcagaaattc cacagttcat cgccctggaa gatgtcggga acgccggggg aggtcagttt
    17161 gaggagggtc tggctcaagg cgttgacggc gccgagcgcg gcgaggggcc gctggaattt
    17221 ttccagatgg gtgaggaaga agttgcgccg gccggcgcgg aggatttcgg cgacgaactg
    17281 ctggaccgcc tcgtcgtaat cgtcgttggg gttgatccag ctcgtgtgtt ccttggcctc
    17341 gcgggtggcc ttgcg
//
LOCUS       DAOPCO010000053        17296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3370_length_17296_cov_5.722638, whole genome shotgun sequence.
ACCESSION   DAOPCO010000053 DAOPCO010000000
VERSION     DAOPCO010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 17296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17296
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3370_length_17296_cov_5.722638"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..829
                     /locus_tag="PLS90_05890"
     CDS             83..829
                     /locus_tag="PLS90_05890"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPK01969.1"
                     /translation="MSQSIHDSPGAAARAAAEQLARLIGDAIAEHGDARIVLSGGPAH
                     AALLDALVAIHGVRWLMVTGYQFSEFVGLPDNHPAAARRTLERFVRRIPGCLPGFHFL
                     RPGLDPLAERDRLSDFISREPIDAAVVDLQPDGSIGFNQPPAEFETRAGYALVPLAEP
                     TRRRLALAGLFESPDRTPRRAITMTLPQLLKARTILALATGDCAAPVAAAISGPVDAA
                     VPCSILSRHPRCELFLDREAARGLNVPTAS"
     assembly_gap    1102..1201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1202..1463
                     /locus_tag="PLS90_05895"
     CDS             <1202..1463
                     /locus_tag="PLS90_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01970.1"
                     /translation="IPMYGFMFADKTEVFREQLDAAGAAFLPYYAHEPDYGITLLAPA
                     YVRAALPEDGDPPIRLLRHAERGWDNHQDVWSFQRLAPPSSS"
     gene            1600..3861
                     /locus_tag="PLS90_05900"
     CDS             1600..3861
                     /locus_tag="PLS90_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01971.1"
                     /translation="MRSFTILVCLLLVVTRPVNAVGESVTAELNGLQLAFDADSGALI
                     EMTYPGVGAMIQTSAARGSLLDLAYPIPSFEPLRLAARFSTGASIERTSDSVTVRWER
                     LGPSRRRTPQSPVREAIALRYDAENDRQEWVTLAEDPFELQGNVAASVTFRALPDGRS
                     LSMTGTVDNQSATTIPQVLFPDFMGITEFAGQGRTHFRTAGFVNNPFELLRTPARGID
                     FYGQDLARTGIEYASGPYYPSTPMLARWMDVGGLQGGFSLFRKWWLWGPGDNWQDSPE
                     KVWLHLTEGEDKLRLACLHKVEIGPGERWHSDEYVLTPHRHGWAKGIEPFREYVHANL
                     KRPYPVPRHVREGLGFRTLWMTQAYPNDPDGDIIYRAEDLPRVARECLEHGIQEICLW
                     SWWGGMQVPISDPFPQIGTVEQLRRAIEECRELGVNVSLFISIMLVANPTAERWGLQV
                     GPPGSGWTYHTEFVPVMRPYYGHGLSCIEAGIDNLTWRQGAYESCRRIIETLTPSLGW
                     DQWDLYSRFDQPNQPRKQPFYELTQRIRAAAHAQDPEATFHGECIENLDANAIYLDYS
                     WNWVNWPWMEGGDCRPFNSVYPAPRLNVNIDRSAREVRGAFIDNHYLNVFTSRVGGIN
                     GSGRIADYPELSRALKQCAGLRRQFLPYFVDGTLIGDCILTEEPAGARLSAYVLPDRL
                     LVLALNDSETGQRIPLAADLTPWLRSPTGQYEVRRFDLEGRLLASEINGSRWRPTLGD
                     AEMVVYEILARPD"
     gene            4204..4395
                     /locus_tag="PLS90_05905"
     CDS             4204..4395
                     /locus_tag="PLS90_05905"
                     /inference="COORDINATES: protein motif:HMM:NF015554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein"
                     /protein_id="HPK01972.1"
                     /translation="MTRLLVAIILVAVLFGLTAVAGLVWAIRRGQFRNLTAGARSIFD
                     NEEPVGRPTDAFPPPREEE"
     gene            4434..5918
                     /locus_tag="PLS90_05910"
     CDS             4434..5918
                     /locus_tag="PLS90_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPK01973.1"
                     /translation="MVLAVQDPSEGADPRQELRSRAEIDRSCQGPVTLFVASAVGWLL
                     FGSLLAMAASIKLHSPGFLSHHAWLTFGRVRPAHLNTVIYGWAAMVGIAAMLWLQTRL
                     SRVTLVFPRLLTAIAWLWNLAVAAGTVAILAGHGTGVEWIEFPVPVALALALLFGAVG
                     LVSFYTFARRPPGHIYVTQWYLFGAILWFPYLYLMAQLMIHLGGATGVVQGSANWWFA
                     HNVLGLFLTPIGVGAVYYLIPKVIGRPIHSYYLSLLGFWSLALFYNWAGTHHLVGGPL
                     PAWLISVGIVGSVMMFIPVTAVAINHHLTMVGHFHMLRTSPTLRFVVFGGMSYTLVSF
                     QGSLQALRGVNEISHFTHYTVAHAHLGVYAFYSMVMFGAVYYMLPRMTGREWSSARLI
                     RIHFWGSALGIALYWGGLTWGGWLQGRMMNDAAIPFLDIVRFTIPFLWSRSFAGVLMT
                     IGHVAFAVLVWRMLAGAREPARAPGPTLLGRPRREREINPAEAQ"
     gene            5918..6532
                     /locus_tag="PLS90_05915"
     CDS             5918..6532
                     /locus_tag="PLS90_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HPK01974.1"
                     /translation="MNRTALLYAGIIFTVIISSIGLVFIPEYQLFRLEPVEAADGNLY
                     PQPRTEFEEAGRRVYIAMGCVYCHSQQVRPEHFGADIDRGWGTRRSVARDYLYDQPHQ
                     LGTMRTGPDLVNIGSRQSDDNWHYQHLYDPELTSPGSTMPKFPFLFEKRTLEPGREPP
                     FNAIRMPPGVLAENEYLLPTTDAQQLVAYLKSMRHDYAVPEAQP"
     gene            6529..7191
                     /locus_tag="PLS90_05920"
     CDS             6529..7191
                     /locus_tag="PLS90_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPK01975.1"
                     /translation="MTTNPHQTFIRDTADADVEAIHEPIAREKAEPRDGYEPISIWLV
                     FLFLALAGWGGWYLGAFSGDWNAMVYDERAQAGAAPAAEAKAPDMLTLGRRAYVRCQA
                     CHQPTGQGLPGTYPPLAGSPYATGPAERAAAIVLNGLQGPLVIDGETFDNIMPAHRDL
                     MTDLEIAAVLTYVRQSWGNDAPAVAPEDVARVRAATAERGAAWTAGELDQFALPEPEP
                     AE"
     gene            7255..9321
                     /locus_tag="PLS90_05925"
     CDS             7255..9321
                     /locus_tag="PLS90_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPK01976.1"
                     /translation="MNPSPDPAACCPPLPRDPAQERQALRDWWRIGLGTLLAGYAMSF
                     SLAINTAEVSATTRTAVHTGLLIVAVILWATLGGPLLRRAFQAARARRVTVEALFLAG
                     LLGASGASLQGMLAGDGPVYFETVAVLLVVYSLGRRLTQSSQERALASVASWLGIPES
                     CERLAPGGTSRACPVREIAAGDLVRVQPGRMIPVDGIVAAGAAFVQQAHVTGESSLAR
                     RGPGDAVAAGAVCVDATLTVRATAPGNARRIDRIARAIDQARARPSAFVTAADRMARL
                     FLPLVLAIALLTFLVWTRLAGWPTGLFNMLAVLLIACPCALGFATPAAVWSAIGRIAE
                     HGLVARAGSLIERLAAVDLVIFDKTGTLTLPRPQLADLVLADASPGTHRRWRALIAAA
                     ERASDHPLAEALASLAPAADAIADAVRVDALRIIPGLGIEADVTWRADGETGSGCLRI
                     GSEGIVGADQKAPWRELSDRLQAGDLTQRIAVLLDGRPIATAAIDERPMPSMQPALEL
                     TRALGPRLMVLTGDTADRAARLGIAEYEAALTPEAKLARVRELRAAGRTVLFVGDGLN
                     DAAAMAEADAAIAVAGAAPLARESADALLTDDDLAAIPRAIDLARRAARVIQSNIRLA
                     VAYNLAGMAVAAAGWLHPVAAALIMTCSSLIVTWRSLRALHDEPGAAPVKNPAPEARH
                     AVLPAP"
     gene            9299..10105
                     /locus_tag="PLS90_05930"
     CDS             9299..10105
                     /locus_tag="PLS90_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01977.1"
                     /translation="MPSSPRRDRRLALTLGAAVLAQGLGLVLLDRPPAPAAAQWLLCA
                     AALALLFQQAWRHRWHLNHRVDMLLIMLAVGGLGMLAGTWIDEQVAARATGNPRPEPP
                     AAGPAAGGDMTIHPPAGHAAHLSHAAHGLPSPHGGHQHSGHQHGGLSYGSLRDAAFSW
                     MTALMLLGAIPPAVAFTRCAALARADRRRWFSTHIAGNAGMVVGMLLLGALLAPSLAR
                     MFGSARAAGHVAMLIGMAAGMLGAQLAAELLLGLKPWRRATGLVSANGTP"
     gene            complement(10118..11890)
                     /locus_tag="PLS90_05935"
     CDS             complement(10118..11890)
                     /locus_tag="PLS90_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012623912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPK01978.1"
                     /translation="MSKSQPASGGGRDGAPERGQDGGAAAAGGADRPAAPLDFIRSRI
                     AEDVRAGLHGGRVATRFPPEPNGYLHIGHAKSMCLNFGVAEEFGGECHLRFDDTNPTK
                     EEQEYVDAIQEDIRWLGFDWGEHLYFASDYFDQLYEWAVKLIREGKAYVDDLSAEEIR
                     EYRGTLTEPGRESPYRNRTVEENLDLFERMRAGEFESGTRTLRAKIDMASGNLNLRDP
                     VMYRILKAPHHRTGTRWCIYPMYDWAHGQSDAIERVTHSICTLEFADHRPLYDWYIEQ
                     LGVFPSRQYEFARLNLTYTVMSKRRLLTLVREGYVNGWDDPRMPTLCGMRRRGYTPES
                     IRDFCARIGVAKAGNTVEIQMLEHCLREDLNARAPRFMAVLRPLRVVIENYPEGQVEE
                     LEAVNNPENPAAGTRRVPFSRELYIEREDFMEDPPKKFYRLAPGREVRLRWGYFITCR
                     DVVKDERGEVVELRCTYDPATRGGSAPDGRKVKATLHWVSAARAVDAEVRLYEHLFTK
                     ENPDDVDEGRDWTDNINPHSLEIVTGCKLEPSLRDAAPGMHVQFERLGYFCADPDARP
                     GAPVFNRTVTLKDTWARIASRADG"
     gene            complement(12066..12665)
                     /locus_tag="PLS90_05940"
     CDS             complement(12066..12665)
                     /locus_tag="PLS90_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPK01979.1"
                     /translation="MRPDEIEVERHRRPEHAIEPLFYHRWSPRAMSGEGLDQATLLRL
                     FEAARWAPSSYNNQPWRFIYAHRGTKDWDRLFGLLVEGNAQWAHAAAVLVVIASKKVF
                     DYNGKPSVTHAFDTGAAWQNLALQASLLGLVAHGMQGFDYERARTELGVPDEYSVEAM
                     VAIGHPGNIEDLPENRREQELPSDRKKLSEIVMEGRFRA"
     gene            complement(12743..13156)
                     /locus_tag="PLS90_05945"
     CDS             complement(12743..13156)
                     /locus_tag="PLS90_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPK01980.1"
                     /translation="MARLRGTVETCLYVDDLERAAAFYSDVLGMRAMTGDERFRALEI
                     APGHVLLLFVRGGTLEPVDTGNGIIPPHDGGGPVHVGFAIDLADLVEWERRLAEHRVP
                     LESRVARPRGGRSIYFRDPDGHLVELLTPGVWPNY"
     gene            complement(13251..14795)
                     /gene="zwf"
                     /locus_tag="PLS90_05950"
     CDS             complement(13251..14795)
                     /gene="zwf"
                     /locus_tag="PLS90_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HPK01981.1"
                     /translation="MARIKPHVTIRGDFCIETPPQPCGLVIFGSSGDLTSRKLMPALF
                     NLFRRRLLSRHFYILGFARTAPDDDTFRLGMRDELMRNTKKPATADLDAFLSHVYSMA
                     GGYTSDEDHQRLRQRLATLDQEHGTRENHLFYLATPPTVYAPLVACLARAGLASEPRT
                     GAGCSRVIIEKPFGRDLTSAIALDDSLHQVLAEQQIYRIDHYLGKETVQNILMFRFAN
                     AIFEPIWNRGYIDHVQITVAETLGVGHRAGYFEQAGVLRDMFQNHMLQMLSLVAMEPP
                     ASFEADRVRDEKVKLLRSIRRMPLDAMDDWLVRGQYGAGEQEGRRVRGYREEDGVDPE
                     STVETYVAARLMIDNWRWRGVPFYLRSGKRLARRVSEIAVYFKPVPHSMFQPIAPEEL
                     VPNILVLTVQPDEGIALQMQAKHPGPKLCMSSLMMDFRYREVFDEEPGEAYERLLLDA
                     MLGDQTLFVRRDDMEVAWSLITPVLERWEAEGKAGAIHEYAPGSWGPAAADGLLARDG
                     RAWHIG"
     gene            15160..16329
                     /locus_tag="PLS90_05955"
     CDS             15160..16329
                     /locus_tag="PLS90_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01982.1"
                     /translation="MEPPRLSILIACHQQGDLLPDCLHSVLAQRGAFEAIVVNDGSTD
                     ATSAVVRELQTRHAGKLHLIEQENRGLSAARQAGFSLARGKYILCLDADDLLEPGAVE
                     AVISTLDAHPEAEAAVGNALRVAADGRTILSELAQTPGRGAPWPPRWPDVLRVNPFGA
                     GMAVACRASAIRRVGGLEVPGVRRCEDWDLWVRMVRCGMHFVPIERVLGRYRQHAATL
                     SRQPVGLARDKLALLEKAAAPDPRLHGLGLRPAGPISPALRAELANLHVAHSLGLLAG
                     QLRADPAARTWPAQLEELLTLIHPDAWPAAAAAEYFLWGWQSGLAGGVRPPRPAQVRR
                     RILRPLVHRLGRAGPEAARRIDELTLPPSLARRIARRLARAWPDRGHGRGSLFKN"
     gene            16417..>17296
                     /locus_tag="PLS90_05960"
     CDS             16417..>17296
                     /locus_tag="PLS90_05960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK01983.1"
                     /translation="MSDLAPSVSVIICTHNPNRTYLHQTIEAILRQSLPPDQWELIVV
                     DNNSDPPLESWLDLSHAPNARIVRELNLGLTHARMRGIREARAELTLLVDDDNVLRHD
                     YLEHAVRIMTQKPFLGAIAGKCKGELEKHPPRWIRLFYHYLAILDHGDQARWINHPNT
                     FDVWYPRGAGLVLRTELAMDYVRQLELQPERQNFDRRGRSLASAGDIDIVMTVMDLGY
                     AVGYFPQLVLRHIIPASRMTFRYMRRMIFSAHYSTFQLYTLRGCQKPLRPWPLGYAAS
                     IVLCLLHGYLHPLTWLL"
BASE COUNT         2500 a         6451 c         5316 g         2929 t
ORIGIN      
        1 ccgggttgaa tctcgttcgc attcgccgcc gaaccgggta aggtgggagg ttgtgacatt
       61 cgcagcgaag gagtcagctc ccatgtcgca gagcatccac gacagtcccg gcgccgccgc
      121 ccgcgccgcg gccgagcagt tggcccgcct gatcggcgac gccatcgcgg agcacggcga
      181 tgcgcgcatc gtgctctcgg gcggtccggc ccacgccgcg ctgctcgacg ccctcgtcgc
      241 catccacggc gtccgctggt tgatggtgac cggctatcag ttcagtgaat tcgtcgggct
      301 gcccgacaat caccccgccg ccgcgcgccg gacgctggag cgcttcgtgc gccggatccc
      361 cggctgcctc cccggtttcc acttcctccg gccgggtctc gatccgctcg ccgaacgcga
      421 ccggctgtcc gacttcatca gccgcgagcc gatcgacgcg gcggtcgtcg atctccagcc
      481 cgacggctcg atcggcttca atcaaccccc cgcggagttc gaaacgcgcg ccggctacgc
      541 cctggttccc ctcgccgaac ccacccgccg gcgcctggcc ctcgccgggc tgtttgaatc
      601 gcccgaccgg actccccgcc gcgcgatcac gatgacgctg ccccaactgc tcaaggcgcg
      661 cacgatcctc gcgctggcca cgggcgactg cgccgctccc gtcgccgccg cgatctccgg
      721 tccggtcgac gccgccgtcc cctgctcgat cctgagccgg catccgcgct gcgagctctt
      781 cctggatcgc gaagccgcca ggggattgaa tgtgccgacg gcgtcatgat gcccccgcgc
      841 ccccattctt cccatctgcc cactcccctg aggcgaacgg ctcccgcggc ggcggcggaa
      901 cgattcgcat cgtgcacaag ctgacccgcg cgcgaggagc ccgcatggat tggatcaccg
      961 ctttccagaa catctacggc cgggtcaagt ccccctggat gttcgccttc aatcaggaaa
     1021 tctacgacca cgaggggcgg cggcgcgatc cgctgcgcga aaagcagacc cgctggggga
     1081 tgcgcctgac gacgcgcctc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 ngatcccgat gtatggcttc atgttcgcgg acaagaccga agtgttccgc gagcagttgg
     1261 acgccgcggg cgccgcgttc ctgccctatt acgcgcatga accggactat ggcatcacgt
     1321 tgctggcgcc ggcctatgtc cgcgcggcgc tgcccgagga cggcgacccg ccgatccgcc
     1381 tgctgcgcca tgccgagcgc ggctgggaca accatcagga cgtctggagc ttccagcgcc
     1441 tcgcccctcc ttcgtcctcg tgatggccca tggagtttca gcatgcccgc cccgaggcat
     1501 cttttcgcac tcacagatac tcctcgactc cgattgccgg attgctacag tgatccccgt
     1561 cagccatctt ccagcccgcg gttgaacagg aggaggccga tgcgatcctt cacgatcctg
     1621 gtctgtctgc ttctcgtggt cacacgaccg gtcaacgccg tcggcgagtc ggtcacggcc
     1681 gagttaaacg gcctgcagct tgccttcgac gccgactcgg gcgccctcat cgagatgacc
     1741 tatcccggag ttggcgccat gatccagacg agcgccgcgc gcggcagcct gctcgacctg
     1801 gcctacccca ttcccagctt cgagccgctg cggctcgccg cgcgcttctc gacgggcgcc
     1861 tccatcgaac gcacctcgga cagcgtgacc gtccgttggg agcgcctggg gccaagccgc
     1921 cgccgcacac cgcagtcccc ggtccgcgag gccattgcct tgcgctacga cgccgagaac
     1981 gaccgccagg agtgggtgac gctggcggag gatcccttcg agcttcaggg aaacgtcgcg
     2041 gcgtccgtca cgttcagggc tctccccgat ggccgctcgc tgtcgatgac cggcaccgtc
     2101 gataatcaat ccgccacgac catcccgcag gtcctctttc ccgatttcat gggcattacc
     2161 gaattcgccg gccaggggcg aacgcacttc cgcaccgccg gtttcgtgaa taaccccttt
     2221 gaactgctcc gcaccccggc ccgcggcatc gatttctacg gccaggatct cgcccgcacg
     2281 gggatcgaat acgcttcggg accgtattac ccgtcgacgc cgatgcttgc gcgctggatg
     2341 gacgtgggcg ggctccaggg cggtttcagc ctgttccgca aatggtggct gtgggggccg
     2401 ggcgacaact ggcaggactc gcccgaaaag gtgtggctgc acctgaccga gggtgaggat
     2461 aagctgcgcc tggcctgcct gcacaaggtg gagatcgggc ccggcgagcg ctggcacagc
     2521 gacgagtatg tcctgacccc ccaccgacac ggctgggcca aggggatcga gccgttccgt
     2581 gagtacgtcc acgccaacct gaagcgcccg tatcctgtcc cgcgacacgt gcgtgagggg
     2641 ctcggcttcc ggaccttgtg gatgactcag gcctatccca acgatcccga cggcgacatc
     2701 atctatcgtg ccgaggacct gccccgcgtg gcccgcgagt gcctcgagca cggcatccag
     2761 gagatctgcc tgtggtcgtg gtggggcggc atgcaggtgc cgatttcgga tcccttccca
     2821 caaatcggca ccgtcgagca actgcgccgg gccatcgagg aatgccgcga gctgggcgtc
     2881 aacgtcagcc tgttcatctc gatcatgctg gtggccaacc caacggcgga acgctgggga
     2941 ctgcaggtcg ggccgccggg ctcgggatgg acctatcaca cggaattcgt tcccgtcatg
     3001 cgcccgtatt atggccacgg cctctcgtgc atcgaggccg gcatcgacaa tctgacctgg
     3061 cgccagggcg cctatgaatc ctgccggcgg attatcgaga cgctcacccc ttcgctcggc
     3121 tgggatcagt gggatcttta ctcacgcttc gaccaaccca accagccgcg caaacagccg
     3181 ttctatgagt tgacgcagcg gatccgcgcc gccgcgcacg cccaggatcc cgaggcgacg
     3241 ttccatggcg agtgcatcga gaacctggac gccaacgcca tctatctcga ctactcgtgg
     3301 aactgggtca actggccatg gatggagggg ggtgactgcc ggccgttcaa cagcgtttac
     3361 cccgccccgc gcctgaatgt caatatcgac cgttcggcgc gggaggtgcg cggcgccttc
     3421 atcgacaacc actacctcaa cgtcttcacg tcgcgcgtcg gtgggatcaa cggctcgggc
     3481 cggatcgccg attatcctga actgagccgg gccttgaagc agtgcgccgg gttgcgcagg
     3541 caattcctcc cctattttgt tgacggcacc ctgatcggcg attgcatcct caccgaagaa
     3601 ccggccggtg cccgtctaag cgcctacgtg ctccctgatc gcctgctggt gctggccctg
     3661 aacgacagcg aaaccgggca acggatccca ttggcagcgg atctgacgcc gtggctgcgc
     3721 tcgccgaccg gtcaatatga agtgagacgg ttcgatctgg aaggacgtct gctggcttca
     3781 gagattaacg gttcccgatg gcgcccaacc cttggcgacg ccgagatggt ggtttacgaa
     3841 atcctggcgc ggcctgattg acccccgagg ggtctgctcc ccgggaccac aggcccgcga
     3901 cccgccagac gggttcactc gccccggagc accggctgtg cgggagggat gcgatccgaa
     3961 caccggtcgg atgggatgct cagcatctgg cttgagttcg ccacggccag cccacgggtc
     4021 ggctctgtgg tatcggcggg tttgcagatt ccgccgtcgt gcctgtcccg tgaagatcaa
     4081 ggccattccc cttcaaagcg gaaagccgga aaagggttcc gcttttcgtt ctcagccact
     4141 agaatgggag cgcctcctcg gcgtgggggt ggggcgccgt tgccgaggcg ggggaatcca
     4201 accgtgaccc gtctgcttgt cgccatcatt ctggttgccg tcctgttcgg cctgacggcc
     4261 gtggcggggc tcgtctgggc catccgccgc ggacagttcc gcaacctgac ggcgggcgcg
     4321 cgcagcatct tcgacaacga ggagccggtg ggccgcccta ccgacgcctt tcctcccccg
     4381 cgcgaggaag aataaacccg ccgaaagcga gaatccgccg gactgacgtc gccatggtcc
     4441 ttgccgtcca agacccgagc gagggcgccg acccgcgcca ggagttgcgc tcgcgcgccg
     4501 agatcgaccg ttcctgccag ggaccggtca cgctgtttgt cgccagcgcc gtcggctggc
     4561 tgctgttcgg ctcattgttg gcgatggcgg cctcgatcaa gctgcacagc cccggttttc
     4621 tcagccatca cgcctggctc acgttcggcc gggtccgtcc cgcgcatctg aacacggtga
     4681 tctacggctg ggccgcgatg gtcggcatcg ccgccatgct ctggctgcaa acccgcctct
     4741 cgcgcgtcac gctcgtcttc ccgcgcctgc tgacggccat cgcctggctc tggaatctgg
     4801 ccgtggcggc cggaacggtc gcgatcctgg ccggccacgg caccggcgtc gagtggattg
     4861 agttccccgt tcccgtcgcg ctcgcgcttg cgctgctttt cggcgccgtc ggcctggtct
     4921 cgttctatac cttcgcgcgc cgccccccgg gccacatcta cgtgacgcag tggtatctgt
     4981 tcggggcgat cctctggttc ccttatcttt acctgatggc gcaactgatg atccacctgg
     5041 gcggagccac cggcgtcgtg cagggcagcg ccaactggtg gttcgcccat aacgtcctcg
     5101 gcctgttcct gacgccgatc ggcgtcggcg cggtctacta cctgatcccc aaggtgatcg
     5161 gccgtccgat ccacagttac tatctttcgc tgctgggctt ctggtcgctc gcgctgttct
     5221 acaactgggc cggcacgcac cacctggtcg gcggaccgct gccggcctgg ctgatttcgg
     5281 tcggcatcgt cggcagcgtg atgatgttca tcccggtcac tgccgtcgcg atcaaccacc
     5341 acctgacgat ggtcggccat ttccacatgc tgcggacgag ccccacgctg cgcttcgtcg
     5401 tcttcggcgg catgagctac acgcttgtca gcttccaggg ctcgctgcag gcgctgcgcg
     5461 gcgtcaacga gatcagccac ttcacccact acaccgtcgc ccatgcccat ctgggcgtct
     5521 acgccttcta ctcgatggtc atgttcggcg cggtctatta catgctgccg cgcatgaccg
     5581 ggcgcgaatg gtcctcggcc cgcctgatcc ggatccactt ctgggggagc gcgctcggca
     5641 tcgcgctcta ctgggggggc ctcacctggg gcggctggct tcagggccgg atgatgaacg
     5701 acgcggccat cccgttcctc gacatcgtgc gcttcacgat ccccttcctc tggtcgcgct
     5761 cgttcgccgg cgtgctgatg accatcggcc acgtggcgtt tgccgtgctg gtctggcgca
     5821 tgctggccgg cgcccgcgag cccgcgcgcg cgccggggcc gacgctgctg ggccggccgc
     5881 ggcgcgaacg cgagatcaac ccggcggagg cgcagtgatg aaccgcaccg ccctgctcta
     5941 cgccggcatc attttcaccg tcatcatctc gagcatcggg ctcgtcttca tcccggaata
     6001 ccagttgttc cggctcgagc cggtcgaggc cgccgacggc aacctgtatc cgcagccgcg
     6061 caccgagttc gaggaggccg gccggcgtgt ctacattgcc atggggtgcg tctactgcca
     6121 cagccagcag gtgcggccgg agcacttcgg cgccgatatc gatcgcggct ggggcacgcg
     6181 ccgctcggtc gcccgcgatt atctctacga tcagccgcat cagctcggca cgatgcgcac
     6241 cgggcccgac ctggtcaaca tcggctcgcg ccagtccgac gacaactggc actaccagca
     6301 tctgtatgac ccggaactca catcgcccgg ctcgaccatg ccgaagttcc cgttcctctt
     6361 cgaaaagcga acgctcgagc cggggcgcga gccgccgttc aatgcgatcc gcatgccccc
     6421 cggcgtgctg gctgagaatg aatacctgct gccgacgacc gatgcccagc agcttgtcgc
     6481 ctatctgaag agcatgcgtc acgattacgc cgtgccggag gcgcagccgt gaccacgaac
     6541 ccgcatcaga ccttcatccg cgacacggcg gatgccgatg tcgaggcgat tcacgagccg
     6601 atcgcccgcg aaaaggccga accgcgcgac ggctacgaac cgatctcgat ctggctcgtc
     6661 ttcctgttcc tcgcgctggc cggctggggc ggctggtacc ttggcgcctt cagtggcgac
     6721 tggaacgcga tggtctacga cgaacgcgcc caggccggcg ccgcccccgc cgccgaggcc
     6781 aaggcccccg acatgctgac gctgggacgg cgcgcctacg tccgttgcca ggcctgccac
     6841 cagccgacgg gccagggcct gcccggcacg tatccgccgc tggccggttc gccctacgcc
     6901 accgggccgg ccgaacgtgc cgccgcgatc gtgctgaacg gcctgcaggg gccgctcgtc
     6961 attgacggcg agacctttga caacatcatg cccgcgcatc gcgacctgat gaccgatctg
     7021 gaaatcgcgg ccgtgctaac ctacgtgcgc caatcctggg gcaacgacgc gcccgccgtc
     7081 gcgccggagg acgtcgcccg cgtccgcgcg gccaccgccg agcgcggcgc ggcctggacc
     7141 gccggcgaac tggaccagtt cgcccttccc gaaccggaac cggccgaata acccccccgc
     7201 ggcgccgcga tggatcctcc ggccgtgccg ccggccgcaa ccatgggctg aaagatgaac
     7261 ccgtctcccg accccgccgc ctgctgtccc cccctgccgc gcgatccggc ccaggaacgc
     7321 caggcgctgc gcgactggtg gcgcatcggc ctgggcacgc tgctggccgg ctacgcgatg
     7381 agcttcagcc tggcgatcaa caccgccgaa gtctccgcca cgacccgcac cgccgtccac
     7441 accgggctcc tgattgtcgc cgtgatcctg tgggcgaccc tgggggggcc gctgctgcgc
     7501 cgtgcgttcc aggcggcgcg cgcccgccgc gtcacggtcg aggccctctt tctggccggc
     7561 ctgctcgggg cgtcgggcgc ctcgctgcag ggcatgctcg ccggcgacgg cccggtctac
     7621 ttcgaaacgg tggcggtgct gctggtcgtc tattcgctcg gccggcgcct gacccaatcc
     7681 agccaggaac gggcgctggc gtcggtcgcg tcgtggctcg gcatccccga aagctgcgaa
     7741 cggctcgcgc ccggtgggac ctcccgcgcc tgccccgtcc gcgagatcgc ggcgggcgac
     7801 ctcgtccgcg tgcagcccgg ccgcatgatc ccggtcgacg gcatcgtcgc cgccggcgcg
     7861 gcgttcgtcc agcaggcgca cgtgaccggc gagtccagcc tcgcgcgccg cgggccgggc
     7921 gacgccgttg cggccggcgc ggtttgcgtc gacgccacgc tgaccgtgcg cgccaccgcg
     7981 cccggcaacg cccggcggat cgaccgcatc gcccgcgcga tcgaccaggc gcgcgcccgc
     8041 ccgtcggcct tcgtcaccgc cgccgaccgc atggcgcgcc tgttcctgcc gctggtcctc
     8101 gcgattgcgc tgctgacgtt cctcgtctgg acccggctgg ccggctggcc gaccggcctg
     8161 ttcaatatgc tggcggtcct gctcatcgcc tgcccctgcg cgctgggctt cgccacgccg
     8221 gccgccgtct ggagcgccat cggccgcatc gccgagcacg gcctggtcgc gcgcgccggt
     8281 tccctcatcg agcggctcgc cgccgtcgac ctcgtgatct tcgacaagac cggcacgctc
     8341 accctgccgc ggccccaact ggccgatctg gtcctggccg acgcgtcgcc cgggacgcac
     8401 cggcggtggc gcgccctgat cgccgcggcc gaacgggcgt cggatcatcc cctggccgag
     8461 gcgctggcct cgctcgcgcc cgccgccgac gcgatcgccg acgccgtccg ggtcgatgcc
     8521 ctgcggatca tccccggcct ggggatcgaa gcggacgtga cctggcgcgc cgatggcgag
     8581 acgggctcgg gttgcctgcg catcggttcg gaaggcatcg tgggcgctga tcagaaagca
     8641 ccatggcgcg agctgagcga ccgcctgcaa gcgggcgacc tgacccagcg catcgccgtc
     8701 ctcctcgacg gccggccgat cgccaccgcc gccatcgacg agcgcccgat gccttccatg
     8761 caaccagcct tggagctgac ccgcgcgctc ggtccgcggt tgatggtgct gaccggggac
     8821 acggccgacc gcgccgcccg gctcggcatt gccgagtacg aggccgccct cacgccggag
     8881 gcgaagctgg cccgcgtccg cgagctgcgc gccgccggcc gcactgtgct gttcgtcggc
     8941 gacggtttga acgatgccgc cgccatggcc gaggccgacg ccgccatcgc cgtggccggc
     9001 gccgcgccgc tcgcccgcga atcggccgac gccctgctga cggacgacga cctggcggcg
     9061 atcccccggg cgatcgatct cgcgcgccgc gccgcccgcg tgattcagag caacatccgc
     9121 ctcgccgtcg cctacaacct ggccgggatg gccgtggccg ccgccggttg gctgcatccg
     9181 gtcgcggcgg cgctcatcat gacctgctcc agcctgatcg tcacttggcg ttcgctgcgc
     9241 gcgctgcacg acgaacccgg cgctgcgccg gtcaagaacc cggctccgga ggcgcgccat
     9301 gccgtcctcc ccgcgccgtg accgccggct cgcgctgact ctgggcgcgg ccgtcctcgc
     9361 ccaggggctc ggcctggtgc tgctggaccg cccgcccgcg ccggccgccg ctcaatggct
     9421 gctgtgcgcc gccgcgctgg ctctgctctt tcagcaggcg tggcgtcacc gctggcatct
     9481 caatcatcgc gtcgacatgt tgctgatcat gcttgcggtc ggcggcctgg gcatgcttgc
     9541 cggcacgtgg atcgatgagc aagtcgccgc gcgcgcaacc ggcaacccgc gtccggaacc
     9601 gccggcagcc gggccggcag ccggcggcga catgacgatc catccccccg ccggccacgc
     9661 cgcgcacctt tcgcatgctg cacacggctt gccgtcaccc cacggcggcc atcaacacag
     9721 cggccatcag cacggcggcc tgagctacgg ctcgctccgc gacgccgcgt tcagttggat
     9781 gactgctctg atgctgctgg gcgcgatccc gccggcggtc gccttcacgc gctgcgcggc
     9841 gctggcccgt gccgaccgcc gccgctggtt ttcaacccat atcgccggca atgccggcat
     9901 ggtcgtcggc atgctgctcc tcggcgcgct gctcgccccg tccctcgcgc ggatgttcgg
     9961 ttccgcccgc gccgccggcc acgttgcgat gctcatcggc atggccgcgg gcatgctggg
    10021 cgcgcagctc gccgccgaac tgctcctcgg gctcaagccc tggcgccgcg cgaccggcct
    10081 ggtctccgcg aacggaaccc catgatccgc cgcgggttca accgtcggcg cggctcgcga
    10141 tcctggccca cgtatccttc agcgtcaccg tgcggttgaa caccggcgcg cccggcctcg
    10201 catccggatc ggcgcagaag tagcccagcc gttcgaattg cacgtgcatg ccgggcgccg
    10261 cgtcgcgcag gctcggctcc agcttgcacc ccgtcacgat ttccaatgaa tggggattga
    10321 tattgtccgt ccagtcccgc ccctcgtcca cgtcgtccgg attttccttc gtgaacaggt
    10381 gctcgtagag ccgcacctcc gcgtcgacgg cgcgcgcggc cgagacccag tgcagcgtcg
    10441 ccttcacctt ccggccgtcg ggcgccgaac ccccgcgcgt cgccgggtca tacgtgcagc
    10501 gcagctcgac aacctcgcct cgctcgtcct tcacgacgtc gcggcaggtg atgaaatagc
    10561 cccagcgcag gcggacctcg cgccccggcg ccagccgata gaatttcttc ggcgggtctt
    10621 ccatgaagtc ctcgcgctcg atatacagtt cgcgcgaaaa cggaacccgc cgcgtccccg
    10681 ccgccgggtt ctccgggttg ttcaccgcct ccagttcctc cacctggccc tcggggtaat
    10741 tctcgatcac gacgcgcagc ggccgcagca ccgccatgaa gcgcggcgcc cgcgcgttca
    10801 ggtcctcgcg caggcagtgc tccagcatct ggatctccac ggtgttgccc gctttcgcca
    10861 cgccgatccg ggcgcagaaa tcgcggatcg actcgggcgt gtaaccccgc cggcgcatcc
    10921 cgcagagcgt cggcatccgc gggtcgtccc agccgttgac gtagccctcg cgcaccagcg
    10981 tcagcagccg gcgcttgctc atcacggtgt aggtcaggtt cagccgcgca aattcatact
    11041 gccgcgaggg aaacacgccg agctgctcga tataccagtc atacagcggc cggtgatcgg
    11101 cgaactccag cgtgcagatc gaatgcgtca cgcgctcgat tgcgtcggac tgcccgtgcg
    11161 cccagtcata catcgggtag atgcaccacc gggttccggt ccggtgatgc ggcgccttca
    11221 ggatgcgata catcaccggg tcgcgcaggt tcagattgcc cgaagccatg tcgatcttgg
    11281 cccgcagcgt gcgcgttccg ctctcgaact cgcccgcgcg catccgttcg aacagatcga
    11341 ggttttcctc aaccgtccgg ttccggtagg ggctttcccg tccgggctcc gtcagcgtgc
    11401 cgcggtactc gcggatctcc tcggcgctca ggtcgtccac gtaggccttg ccttcgcgga
    11461 tcagcttgac cgcccactcg taaagctgat cgaaatagtc cgaggcgaaa tacagatgct
    11521 cgccccagtc gaacccgagc cagcggatgt cctcctggat cgcgtcgacg tattcctgct
    11581 cttccttcgt cggattggtg tcgtcgaacc gcaagtggca ctcgccgccg aattcctccg
    11641 ccacgccgaa attgaggcac attgatttgg catgcccgat gtgcagatag ccgttcggct
    11701 cgggcgggaa gcgcgtcgcg acccgtccgc cgtgcagccc cgcgcgcacg tcctcggcaa
    11761 tccggctccg gatgaagtcc agcggcgcgg cgggccggtc cgcgccgccc gcggccgccg
    11821 ctcccccgtc ctggcctcgt tccggcgcgc cgtcgcgacc gccgccgctg gctggttggc
    11881 ttttgctcat ccctcgctcc cgttgcgcaa gatcaccatg acccggtccc tgtcatccct
    11941 gccgggatga tgcacagcgc cgcaatctag cacacgcgga cggccgttgc cagccgcggc
    12001 gggcccgcgc acacaggcgc ttgctgagaa ccgtccggct ccgtccgccg cgaccggttc
    12061 ccgtctcacg cgcggaaccg gccctccatc acgatttcgc tcagcttctt gcggtcgctc
    12121 ggcagctcct gctcgcggcg attctccggc aggtcctcga tgttgcccgg gtggccgatc
    12181 gccaccatcg cttccacgct gtattcgtcc ggcacgccca gttcggttcg cgcccgctcg
    12241 tagtcgaacc cctgcatccc gtgcgccacg agccccagca gcgacgcctg cagcgccagg
    12301 ttctgccagg ccgcgcccgt gtcgaacgcg tgcgtcaccg acggcttgcc gttgtagtcg
    12361 aacacctttt tcgacgcgat caccaccaga acggccgcgg catgcgccca ctgggcgttg
    12421 ccttccacca gcagaccgaa caatcggtcc caatccttcg ttccgcggtg ggcatagatg
    12481 aaccgccacg gctgattgtt gtaagaggac ggcgcccagc gcgcggcctc gaacaatcgc
    12541 agcagcgttg cctgatccag cccttcgccc gacatcgccc gcggcgacca ccggtggtaa
    12601 aacagcggct cgatcgcgtg ctccggccga cgatggcgtt ccacttcaat ctcgtccggt
    12661 cgcatggtct gacctccttg cggttgttgc atcaccttca gaccgggcgc gcgcaaaaat
    12721 cgttcctgct ccgcacgcgg cttcagtagt tgggccagac ccccggcgtc agcagctcga
    12781 ccaggtggcc gtcgggatcg cggaagtaaa tgctgcggcc gccgcgcggc cgcgccacgc
    12841 ggctctccag gggcacgcgg tgctcggcca gccgcctctc ccattccacc aggtccgcca
    12901 gatcaatggc gaagccgacg tggaccgggc cgccgccgtc gtgcggcggg atgatgccgt
    12961 tgcccgtgtc gaccggctcc agcgtgcccc cgcgcacgaa cagcagcagg acgtggcccg
    13021 gcgcgatctc gagcgcgcgg aaccgctcgt cgccggtcat cgcgcgcatc ccgagcacgt
    13081 cgctgtaaaa cgcggcggcg cgttccaggt cgtcgacgta caggcacgtt tccaccgttc
    13141 cccgcaaccg cgccatcgcc ttcgcctccc gtctcgcttc aagtcgtctc tcgtcctcgc
    13201 actcgtcctc gtcctcgccc gcaaaaactc gtcctcgtcc tcgctgccgc tcacccgata
    13261 tgccacgccc gcccgtcgcg cgcgagcagt ccatccgccg cggccggccc ccatgacccc
    13321 ggggcgtatt catgaatcgc gcccgccttc ccctccgcct cccagcgctc cagcaccggc
    13381 gtgatcagcg accacgccac ctccatgtcg tcgcgccgca cgaacagcgt ctggtcgccc
    13441 agcatcgcgt cgagcagcag gcgttcatac gcctcgcccg gttcctcgtc gaagacctcc
    13501 cgataccgga aatccatcat cagcgaactc atgcacagct tcgggccggg gtgcttggcc
    13561 tgcatctgca gcgcgatccc ctcgtccggc tgcaccgtca gcaccagaat gttcggcaca
    13621 agctcttcgg gcgcgatcgg ctggaacatc gagtgcggca ccggcttgaa gtagaccgcg
    13681 atctcgctga cgcgccgcgc caatcgcttg cccgagcgca gataaaacgg caccccgcgc
    13741 cagcgccagt tgtcgatcat caaccgcgcc gccacatacg tctccaccgt tgattccgga
    13801 tcgaccccgt cctcctcgcg gtagccgcgc acccggcgcc cctcctgctc gccggcgccg
    13861 tactgccccc gcaccagcca gtcgtccatc gcgtcgagcg gcatccgccg gatcgaccgc
    13921 aacagcttga ccttctcgtc gcgcacccgg tccgcttcga acgacgccgg cggctccatc
    13981 gccaccagcg acagcatctg cagcatgtgg ttctggaaca tgtcgcgcag cacgcccgcc
    14041 tgctcgaagt agcccgcgcg gtgcccgacc cccagcgtct cagccaccgt gatctgcacg
    14101 tgatcgatgt agccgcggtt ccagatcggt tcgaaaatcg cattggcgaa tcggaacatc
    14161 aggatgttct gcaccgtctc cttgcccagg taatgatcga tgcgatagat ctgctgctcg
    14221 gccagaacct gatggagcga gtcgtccagc gcgatcgcgc tggtcagatc gcggccgaac
    14281 ggcttttcaa tgatcacgcg cgaacagccg gcccccgtcc gcggctcgct ggccagcccg
    14341 gcccgcgcca ggcacgccac cagcggcgcg tagaccgtcg gcggcgtcgc caggtagaac
    14401 aggtggttct cgcgcgtgcc gtgttcctgg tccagcgtgg ccagccgctg gcgcaggcgc
    14461 tggtggtctt cgtcgctggt gtagccgccg gccatcgagt agacgtggct gaggaaggcg
    14521 tccaggtcgg ccgtggccgg cttcttggtg ttgcgcatca gctcgtcgcg catccccagc
    14581 cggaacgtgt cgtcgtcggg cgcggtgcgc gcgaaaccga gaatgtagaa gtggcgcgac
    14641 agcagccggc ggcggaacag attgaacagc gccggcatca gcttgcggct cgtcaggtcg
    14701 cccgacgacc cgaaaatgac gagcccgcac ggctgcggcg gggtctcgat gcagaaatcg
    14761 ccgcggatcg tcacgtgcgg cttgatgcgc gccatgcctc ggttccttgt cttggcggga
    14821 ttcgctgccg ggtagcggtt cctccccgga cggatgaaac ccacatggta tcacgcccgc
    14881 cgcgcgccgc aaaccggatc acgccccccc tccaaactcg tcccttgtcc tcggctctcg
    14941 aaatcgggct gtttcctgta ccaaccaccg cctgggaccg ccggcatcct tgccggcatc
    15001 ttgcgcgcca cacaatgagc cctccgtgtc tcggcgtctc ggcgtctcgg cgtgaggtac
    15061 ttttaccaat cggcgatccg aaacggtgac ggaccatcaa cgcgagaggt cgatttttct
    15121 gcctctttgt caatcgccgg tctataaccg actcgcacaa tggaaccccc gcgcctcagc
    15181 atccttatcg cctgtcacca gcaaggcgat ctgctgcccg actgcctcca ctcggtcctc
    15241 gcgcagcgcg gggcgttcga ggcgatcgtc gtcaacgacg gctccaccga cgccacgtcg
    15301 gcggttgtcc gcgagctgca aacccgccat gccggcaagc tccacctgat cgaacaggaa
    15361 aatcgcggcc tttcggcggc ccggcaggcc gggttctccc tggctcgcgg caaatacatc
    15421 ctctgcctcg acgccgacga cctgctcgag ccgggggcgg tcgaggccgt catctccaca
    15481 ctggacgccc atcccgaggc cgaggccgcc gtcggcaatg ccctccgcgt cgccgccgac
    15541 ggccggacca tcctgtccga actggcccag acgcccggcc gcggcgcccc ctggcccccc
    15601 cgctggcccg acgtcctgcg cgtcaacccg ttcggcgccg ggatggccgt ggcctgccgg
    15661 gccagcgcca tccgccgcgt cggcgggctg gaagtccccg gcgtccgccg ctgcgaagat
    15721 tgggatctgt gggtccgaat ggtccgctgc ggaatgcact tcgtccccat tgagcgcgtc
    15781 cttggccgct accggcagca tgccgccacg ctcagccgcc agcctgtcgg cctggctcgc
    15841 gacaaactgg ccctgctgga aaaggccgcc gcccccgacc cgcgcctgca cgggctgggg
    15901 ctccgtcccg ccggccccat ttcgcccgcg ctgcgcgccg agctggccaa tttgcacgtc
    15961 gcccacagcc tgggcctgct ggccgggcaa ttgagggccg accccgcggc ccgaacctgg
    16021 ccggcgcagc ttgaggaact gctcaccctg attcaccccg acgcctggcc ggcggcggcc
    16081 gcggcggagt acttcctctg gggctggcag tccggtctcg ccggcggcgt ccggccgccg
    16141 cggcccgccc aggtgcgccg ccgcattctc cggccgctcg tccatcgcct gggacgtgcc
    16201 ggcccggaag cggcgcggcg gatcgacgag ttgacgctgc cgccctcgct ggcgcgccgc
    16261 atcgcccgcc ggctggcccg ggcctggccc gaccgtggac acggaagggg cagtctattt
    16321 aaaaattaac ccccctctct caatttgaga atgacacttc ccccctaccc gtctatactc
    16381 cttccaacct gagtcttgct gattggacgc ctgctcttgt ctgatctcgc cccctccgtc
    16441 agcgtcatca tctgtacgca caaccccaat cggacctacc tccaccagac gattgaggcc
    16501 attctgcggc agtccctccc ccccgaccag tgggagctga tcgtcgtcga caacaacagc
    16561 gatcccccgc tggaaagctg gctcgacctg tcgcacgccc ccaacgcccg catcgtccgc
    16621 gaactcaacc tgggcctgac ccatgcccgc atgcgcggca tccgcgaggc gcgcgccgag
    16681 ctgactctgc tcgtcgacga cgacaacgtc ctgcgccacg attacctcga gcacgccgtc
    16741 cgcatcatga cccagaagcc cttcctcggc gccatcgccg gcaagtgcaa gggcgaactc
    16801 gaaaagcatc ccccgcgctg gatccggctg ttctaccact atctggcgat cctcgatcac
    16861 ggcgaccagg cccgctggat caaccacccc aataccttcg acgtctggta tccgcgcggc
    16921 gccggccttg tcctgcgcac cgaactggcc atggattatg tccgccagct cgaactgcag
    16981 cccgagcggc agaacttcga ccgccgcggc cgcagcctcg ccagcgccgg cgacatcgac
    17041 atcgtcatga ccgtcatgga tctgggctat gccgtcggct atttccctca gctcgtcctg
    17101 cgccatatta tccccgcctc gcgcatgacc ttccgctaca tgcgccggat gatcttcagc
    17161 gcccattatt ccacgttcca gctctacacg ctgcgcggct gccagaaacc gctccgcccc
    17221 tggccgctcg gttatgccgc cagcatcgtc ctctgcctgc tgcacggcta cctgcacccg
    17281 ctcacgtggc tgctct
//
LOCUS       DAOPCO010000054        16736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3499_length_16736_cov_6.179306, whole genome shotgun sequence.
ACCESSION   DAOPCO010000054 DAOPCO010000000
VERSION     DAOPCO010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16736
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3499_length_16736_cov_6.179306"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLS90_05965"
     CDS             complement(<1..366)
                     /locus_tag="PLS90_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK01984.1"
                     /translation="MPGKKSSNPQLDIRIGTLVNGMQRDVAGYIRAILPHGFESLQLT
                     FNAGVVDLDLKKLAASVKAAIGDADVVISALGIYANPLESGEDDRKAALGWRKLIDAA
                     HLFGAGLVSGFTGRLRGQPI"
     gene            complement(416..1417)
                     /locus_tag="PLS90_05970"
     CDS             complement(416..1417)
                     /locus_tag="PLS90_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK01985.1"
                     /translation="MPRPVTLFTGQWADLPLATLCEKARAFGYDGLELACWGDHFEVA
                     RANRDGKYCAERRKLLDRHGLQLFAISNHLVGQAVCDRIDQRHKAILPPHVWGDGDAE
                     GVRKRAAREMIATAKAARKLGVNIVNGFTGSSIWHLLYAFPPTPDAMIDAGYRDFGKR
                     WKPILDEFDKLGVKFALEVHPTEIAFDIVSAHRALDAVEHHPAFGFNYDPSHLGYQGV
                     DYVAFIREFGDRIWHVHMKDVAWSPAPARSGVFGGHLAFGNPNRYWDFRSLGRGNIQF
                     EPIIRALNDVGYSGPLSIEWEDSGMDREHGAAESCAFTRRLDFAPSKVAFDAAFEKK"
     gene            complement(1505..2263)
                     /locus_tag="PLS90_05975"
     CDS             complement(1505..2263)
                     /locus_tag="PLS90_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK01986.1"
                     /translation="MHLEQQIAVQSYCFRNFKDNREVARMVRDCGLRRIELCGVHAPL
                     REVDAARAAIDEFGECGVEVVSLGVERFANDEPSERALCELARQAGIRAISVDFGLAN
                     MPDSLKTGERLGGEFNLRLGIHNHGGRHWLGSGQILRHICAHAAPAIGLCLDTAWALD
                     AGEDPVALARELADRLYLVHVKDFAFGRDRKPEDVVAGEGNLDVDGLFRVLREADFQG
                     PVILEYEGDVDRPVASVSRCREAVMAAWQRVERG"
     gene            complement(2336..3493)
                     /locus_tag="PLS90_05980"
     CDS             complement(2336..3493)
                     /locus_tag="PLS90_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020529538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK01987.1"
                     /translation="MSRKIRMGMVGGGQGSAIGKVHRMAAALDGQLELVCGAFSSDPR
                     RSKASCAELYLPADRVYDSYAEMFERERRRPEGDRMDFVVIVTPNHLHYAPAAAALKA
                     GFHVMCDKPMTFSLAEARKLKTLVDRTGLLFGLTHNYTGYPMVKEARELARGGKLGAI
                     RKIVVEYPQGWLHELIEATGQKQAAWRTDPARSGAAGCMGDIGTHAENLAEYITGLRI
                     DSLCAELTTFVKGRKLDDDGNVLIRYQGGARGILHASQIAIDEENGLNIRVWGEKGGL
                     QWRQEEPNTLILKRPDGPRQLIRSGQGYLSRRARRACRLPAGHPEGFIEAFANLYRNY
                     AEALRARLAGRKPDPLALDFPTVDDGVRGMAFIEAVVKSARAGAKWVKMAE"
     gene            3797..4864
                     /gene="thiL"
                     /locus_tag="PLS90_05985"
     CDS             3797..4864
                     /gene="thiL"
                     /locus_tag="PLS90_05985"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPK01988.1"
                     /translation="MAAEQNQPPSLAEIGERAWLRRVRRWVERQPDAMRERLVVGVGD
                     DAAVLKLGDPRLVVTTDALIEGVHFRPEWTAPRDLGWKAMAANLSDLAAMAARPVAAF
                     LSLSLPGESLTHDVEALFRGMLEAGRRWGCPLAGGDLTRAPQWCLALTLLGEPMRRGR
                     VARRYTARPGDRLYVTGAPGESGAGLEALRRGLKGKAPAGLIRRHQRPTPRLAEAEVL
                     IHCCRRLTMLDVSDGIWNDASQLAEASGAGIDLEASRLPVSRPLRRLGAALNVDPLDW
                     VLFGGEDYELLFAAGESEERIERAFGAAGLATPVSRIGVVRKGRGVRLLDERGRPIPR
                     ADRTFQHFQESEPWRNPSAKS"
     gene            4837..5400
                     /locus_tag="PLS90_05990"
     CDS             4837..5400
                     /locus_tag="PLS90_05990"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009577636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPK01989.1"
                     /translation="MAQPEREILIERKYLRMVRQGGWEYVERVGTQGVVVIVPVTDDN
                     ELVLTEQYRVPLGQRVIELPAGLAADGGRDGETLEEAARRELIEETGYEARHLERVGA
                     GPTSSGLTNEVVAFYRARGLRKVGRGGGDASEAIEVHTVPLDQIVDWVKRKAAEDRLI
                     EVNVYAGIFFARGFETVADCDTTEERP"
     gene            5400..6857
                     /gene="uxaC"
                     /locus_tag="PLS90_05995"
     CDS             5400..6857
                     /gene="uxaC"
                     /locus_tag="PLS90_05995"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPK01990.1"
                     /translation="MDRPFIHGDFLLQYDDARRLYHEHAAALPIVDYHCHLPPEPIAA
                     DARFETITEAWLYGDHYKWRAMRANGVDERFCTGDASDWEKFQKWAETVPCCLRNPLY
                     HWTHLELARYFGVTELLDGASARPIYERCNAELAQPENSCRGLIRRANVVLVCTTDDP
                     LSDLEAHRRLAEDASFPVRVLPTWRPDRAMAVDDPAAFNEWVDRLAELTGMDIGDFET
                     FLAALERRHQFFHENGCRLSDHGLEEPYAEDYTAGEVAAAFARVRGGQALDAAARLKL
                     KSAMLYEFGRMDHARDWGQQFHFGALRNVNSRMMARLGRDAGFDTIGDFDHARPLARL
                     LDRLEREDRLARTVLYNLNPADNYLFAAMIGNFQDGRTPGKIQFGSAWWFLDQHDGIE
                     LQLEALSQCGLLSRFIGMLTDSRSFLSYPRHEYFRRILCNMLGRDMERGLIPRDDALV
                     GRLIRDVCYHNVVAWLGLDGQALAAADQGGPNWSA"
     gene            complement(6932..7588)
                     /locus_tag="PLS90_06000"
     CDS             complement(6932..7588)
                     /locus_tag="PLS90_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPK01991.1"
                     /translation="MSPQPPASRVAAERLRLIGIADGGWKPWPALQPLAAAALAAGLP
                     ALMVRDRELSDRELLPVAWPLREAARQAGALYILNRHFSLINLLAPDALHIGAGDPAP
                     DQLRDHLGDGLAIGYSAHSEDEALQAIARGADYVVLSPVFSTESKPAATPIGLEPLAG
                     LRRRTSTPIIALGGIRAERIQQVLAAGATGVAMIRAVFGGDPAENVHRLQQAVASAHR
                     "
     gene            7755..8351
                     /locus_tag="PLS90_06005"
     CDS             7755..8351
                     /locus_tag="PLS90_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK01992.1"
                     /translation="MPKRESVIDGPPARPAFESLYAQWSEPVYRYLARLLGAGPIVDD
                     LFQDTWMKAIEHRGQLRDAERFGPWIFRIARNLAFNQRRRGRRRTQVYAVSSLSVAEG
                     QEPESLIESDGEHEAPTPRDAAIDGQRRQLIEAIMEELTDDAREMIHLRYYEQLTLAE
                     VAQVMDVPLGTVCTKVHRSLRTIRRRLERQGHRSLETI"
     gene            8498..9454
                     /locus_tag="PLS90_06010"
     CDS             8498..9454
                     /locus_tag="PLS90_06010"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPK01993.1"
                     /translation="MSQDCTQLQAQLLDDPNGPLDETARRHLATCPVCREVARDARAI
                     GGLLEQPGRDAPPLLPAELREAVGRRIQARLAAGASPRANAARPPATVRTLARAAAAP
                     RFPKWGALAAAALALVILGWALWHPAASARVGQLDFMSGEFRLRAAAGGERAPGALGP
                     GDRLHTAQDCVGWISLGDDREVRVAVAPDTRLSIIDRTRLELEQGQVWLGVEPGGEGF
                     QVATAQAVVRVTGTRFGVVVAGGATQVDVTEGTVQVASPTGRVARLAAGETLSATAAG
                     ELTAPGPRAEGREHPRWVTTAAALEQAHAKARYLPSVGAARE"
     gene            9720..10427
                     /locus_tag="PLS90_06015"
     CDS             9720..10427
                     /locus_tag="PLS90_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK01994.1"
                     /translation="MHRFATGSRCGLTLIELLVTAAIVGILAGIAMANFQRAQVQARV
                     SAGKSQLRTLAGAVERYCVDYGTYPTPAPSFPDDPFGVVAATALASLTTPVAYIAADA
                     LRDPFGTLRLQAGPPSFGDRPRLTSADPFRPPTPGFNMIQSLLFFHYPRFSYLIDQPA
                     MNVHGYASVSVGPDRADSFIVYLPFPSHLPAGASHYGVNTAADTVYDPTNGTVSGGDM
                     AWFGGQLSRGGLIGGAP"
     gene            10448..13201
                     /locus_tag="PLS90_06020"
     CDS             10448..13201
                     /locus_tag="PLS90_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK01995.1"
                     /translation="MVFIPRGASGGIWYRFLRLLRGVLLAGALGIGPAGAADEFVWRP
                     DSPIKDIASPGLVASHAAAADFTGDGLTDLAVTYAFSEQVRLYRGDGEGGFSEYGQLE
                     VGIHEGAGRDLPRQIVIEDFDADGFLDLAILCSGNPDVHAPPSLGICHGAPRGLFEPF
                     EPIELLAENTAADSFPLVLRGARLAGDDAPSLLVGHFDSAQFTVIRHLRGRQWRKPVT
                     FQVDARGAGPADLEPVDLDFDGLTDLIVLNRRDIQFWQGDADGRFGYRGRLEAEFANA
                     AFTAMAPADFDRNGAWDLVVLDGAHSRLLLYLNLAFDADFERRLVYPFTGDIASTNHR
                     EGPIDLVLHDANQDGWDDVAVAFLQSGGGSRLLGRADGAPDPLRLPPLDFGTGPGPRA
                     ILSAHIDADGLPDLVTVNEGAAGEAQHDITVDLAIHGVGGPAPQTGLNAQAPGRGISP
                     YLDRPVGLAWDEERDALWLMDRRRRRLVRLDDDGELQSELPLDSANQAGPLDPMDLTV
                     DPAGELWLADRLGAQVVHLRAHGERAGGFSTLAAGLTRPSGIAHAASPFRLYVSDAAS
                     RRLVAFDAAGAVIQTWERAPMRDLAWDAEHGILWGVPEAEPQVLINLGIASDDDDDDD
                     KTDQPAQLALHEVAPLLKQERIRAIAFGEEGPWILTERGALIETDDDGELKSVRELRL
                     LRDIAAAAPADAGGLWLIDSGLLATLVRIDAGGAVQSRHQLNHPAMSDPIRPLGLARR
                     NERLYLLDGRNGQVRPVEGDRLDAPPVALLPMPPRPSGLCYDESTDSFYSVAPGRLIQ
                     LQPLGPEDGAALASDAVVSWYPLPATHPPSSLTRGREPGELMIYSADRPSVMFLDLQA
                     PGVRLITPLLLNLVDFVPRIVLAEEAAAGVWRLVGSGDEPVQEIRIPTVTSALESGWT
                     RYR"
     gene            13484..14410
                     /locus_tag="PLS90_06025"
     CDS             13484..14410
                     /locus_tag="PLS90_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK01996.1"
                     /translation="MVTRFKKATTFLISVGLALGSVAYAQEQQKPMDQKEQKQEKVLK
                     SDTESGVLIYERQVLATGDGTRLETTIKLPEGIAPKEDARIPAAEEAIRNVISTAVDA
                     ALAGDTDKLIEQLSGPDRERFESQTDADWNPLTKTTGFLNKTWKESYGLATANVTKDD
                     LSATYMAVRIGEIEDGEKLTEWPLEPTPTTNLMQKKGAGTADYMEPGRDVAVVVLPSA
                     NGLPELTVSMTHEAPDFWKIDVPDDMTLKALQESLTLHLNHLNENARSWPADEDETER
                     LITRHVLMGIYGVDAEMDDVEEAQSTTPEPRG"
     gene            complement(14507..16009)
                     /gene="trpE"
                     /locus_tag="PLS90_06030"
     CDS             complement(14507..16009)
                     /gene="trpE"
                     /locus_tag="PLS90_06030"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPK01997.1"
                     /translation="MLKVSPDFETFLQRSREANLIPVWAERLADMETPVSVFRKLADS
                     EHAFLLESVEQGENVGRYSFIGCNPEIIIRCRGHEIDIRYHGDEQPLEQSQPPLEFLR
                     EFMQRYRPAADPQLPPFIGGAVGYMAYDLVRHFEKLPDANPDDLKLPDAYFMISDKLA
                     IFDHVRHRMILLANAHVTDDPRRAYDAAIHKIELMAERLRIEKGERNQPVDAAISPAV
                     ESPVSELVSNFSQDEFMAAVERVKEYIRAGDVFQVVLSQRFSKPYHGDPFDIYRALRA
                     INPSPYNFYLQLGDLKLAGSSPEILVQVNHGEVIVRPIAGTRPRGATPAEDRQHEQDL
                     LADPKELAEHIMLVDLGRNDVGRVGCPGSVHVDDLMAIERYSHVMHIVSNVRGRLRPT
                     SDAFDALAATFPAGTVSGAPKIRAMEIIDELENVRRGPYAGCVGYISFDGNLDTCITI
                     RTAVIKGRRVYIQAGAGIVADSVPATEYQETVNKARAMLRAVEMAEAGLD"
     gene            complement(16220..>16736)
                     /locus_tag="PLS90_06035"
     CDS             complement(16220..>16736)
                     /locus_tag="PLS90_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HPK01998.1"
                     /translation="SESGYGDYDENLRRLARFLDRETSLTVILDDDETLFQPGAAPAA
                     ALYCGWYSLANYVDAFEFVPGAVGYHIASSEAMTLRRDNARNWCPALLRDGVAATLGP
                     VEEPYLTAFPRPTEFFAHLLAGRRSLAEVYALTLPSVSWMMTLIGDPLYRPFAAHPVL
                     DEDQFARLLRE"
BASE COUNT         2680 a         5243 c         6079 g         2734 t
ORIGIN      
        1 gatcggctgg ccgcgcagac gcccggtgaa gccggagacg aggccggcgc cgaacaggtg
       61 cgccgcatcg atcagtttgc gccagcccag ggccgccttg cggtcgtcct cgcccgactc
      121 gagcgggttg gcgtagatcc cgagggcgct gatcacaaca tcggcatcgc cgatcgcggc
      181 cttcacggac gccgccagtt tcttcagatc gaggtcgacc acgccggcgt tgaaagtgag
      241 ctggaggctt tcgaagccgt gcggcaggat cgcgcggatg tagccggcga catcgcgctg
      301 catcccgttg accagcgtgc cgatgcggat atcgagctgc ggattggatg attttttccc
      361 aggcatggtg gtcgcattcc cccacattca aaaatccggc cctcgtccgg tcgcgctatt
      421 tcttctcgaa agcggcatcg aacgccacct tggacggggc gaagtcgaga cgccgggtga
      481 aggcgcagct ctcggccgca ccgtgttcgc ggtccatgcc gctatcctcc cattcaatcg
      541 agagcgggcc gctgtagccg acgtcgttga gcgcgcggat gatcggttcg aactggatat
      601 tgccgcggcc gagcgagcgg aaatcccagt agcggttggg gttgccgaat gccagatgcc
      661 cgccgaagac gcccgagcgc gccggcgccg gcgaccaggc cacgtccttc atgtggacat
      721 gccagatgcg gtcgccgaat tcacgaatga acgcgacgta atcgaccccc tgatagccga
      781 ggtggctggg gtcgtagttg aagccgaagg cggggtgatg ctcgacggcg tcgagtgcgc
      841 ggtgggcgct gacgatgtcg aaggcgattt cggtcgggtg gacctcgagg gcgaacttga
      901 cgccgagctt atcgaattcg tcgaggatcg gcttccagcg cttgccgaaa tccctgtagc
      961 cggcgtcgat catcgcgtcg ggcgtcggcg ggaaggcata gagcagatgc cagatcgagg
     1021 aaccggtgaa gccattgacg atgttcacgc ccagcttccg ggcggccttc gccgtggcga
     1081 tcatctcccg ggcggcgcgt ttgcggacgc cctcggcgtc cccgtcgccc catacatgcg
     1141 gcggcaggat tgccttgtgg cgctggtcga tccgatcgca gaccgcctgg ccgacgaggt
     1201 ggttggagat ggcgaagagc tggagaccat ggcggtcgag gagcttgcgg cgctcggcgc
     1261 agtattttcc atcgcggttg gcccgggcga cttcgaagtg atcgccccag caggcgagct
     1321 cgaggccgtc gtagccgaag gcccgagcct tctcgcacag cgtcgcaagg ggcagatcgg
     1381 cccactggcc ggtgaacagc gtgacgggtc gtggcatgac actacctcct cgggttcatt
     1441 cccgcgcggg atttcctgag ccatcggcga acaggccgtc gcccgttgct ctccgcgcgc
     1501 cgcgctagcc gcgctccacc cgctgccagg ccgccatcac ggcctcgcgg cagcggctga
     1561 cggaggcgac gggccggtcg acatcgcctt cgtattccag aatgaccggg ccttgaaagt
     1621 cggcctcgcg cagcacgcgg aacaagccgt cgacgtcgag gttgccctcg ccggcgacga
     1681 cgtcctcggg cttgcggtca cggccgaaag cgaaatcctt cacgtgaacc aggtagagcc
     1741 ggtcggccag ttcgcgggcg agggcgaccg gatcctcgcc cgcatcgagg gcccaggcgg
     1801 tgtcgagaca gaggccgatc gcgggggcgg cgtgcgcaca gatgtggcgg aggatctggc
     1861 ccgacccgag ccaatgccgg ccgccgtggt tgtggatgcc gaggcggagg ttgaactcgc
     1921 cgccgagccg ctcgccggtc ttgagcgagt cgggcatgtt cgcgaggccg aaatcgacgc
     1981 tgatcgcgcg gatgcccgcc tggcgggcga gttcgcagag cgcgcgctca gacggctcgt
     2041 cgttggcgaa tcgctcgacg ccgaggctga cgacttcgac gccgcactca ccaaactcat
     2101 cgatggcggc gcgggcggca tcgacctcgc gcagcggcgc atggacgccg cacagttcga
     2161 tgcgccgcag gccgcagtcg cgcaccatcc tggcgacttc ccggttatcc ttgaagttgc
     2221 ggaaacagta gctctggacg gcgatctgtt gttcgagatg cattgggggg cttgttcctc
     2281 attgagagaa aatccggtat tcgaatttcg aatttcgata ttcgaatttt ctcccttatt
     2341 ccgccatctt gacccatttc gcgccggcgc gggcgctctt gacgaccgcc tcgatgaacg
     2401 ccatgccgcg cacgccgtca tcaacggtcg gaaaatccag cgccagcgga tcgggtttcc
     2461 tgccggcgag tcgcgcgcgc aacgcctcgg cgtaattgcg atagaggttc gcgaacgcct
     2521 cgatgaatcc ctccggatgg ccggccggca gccggcaggc gcgccgggcg cgcctggaga
     2581 gatagccctg gcccgaccgg atgagctggc gcggcccatc gggccgcttc agaatcagcg
     2641 tgttgggttc ctcctggcgc cattgcaggc cgcccttttc gccccagacg cggatattga
     2701 gcccgttttc ctcatcgatc gcgatctgac tggcgtggag gatgccgcgc gcgcccccct
     2761 ggtagcggat caacacgttg ccgtcgtcgt cgagcttgcg ccccttgacg aacgtcgtca
     2821 gctcggcgca cagggagtcg atccgcaggc cggtgatgta ttcggcgagg ttttcggcgt
     2881 gggtgccgat gtcgcccatg cagccggcgg cgcccgagcg cgccgggtcg gtgcgccacg
     2941 cggcctgttt ctggccggtc gcctcgatca actcatgcaa ccagccctgg gggtattcga
     3001 cgacgatctt gcggatcgcg ccgagtttgc cgccgcgcgc cagctcgcgc gcctccttga
     3061 ccatcgggta gccggtgtag ttgtgggtca agccgaacag caggccggtc ctgtcgacca
     3121 gggttttcag cttgcgggct tcggccagac tgaacgtcat cggcttgtcg cacatgacgt
     3181 ggaagccggc cttgagcgcc gccgcggccg gcgcgtagtg caggtgattg ggcgtgacga
     3241 tcacgacgaa gtccatccgg tcgccctcgg ggcggcggcg ctcgcgctca aacatctcgg
     3301 cgtagctgtc gtaaacgcgg tcggccggca ggtagagttc ggcgcaggaa gccttggaac
     3361 gcctggggtc gctgctgaac gcgccgcaga ccagttcgag ctggccgtcg agcgccgccg
     3421 ccatccggtg caccttgccg atcgccgatc cctgtccgcc gccgaccatg cccatgcgga
     3481 tcttgcgact catcgacctg ctctccaatc gctgaaatta agctgagtga agcgtgcggg
     3541 taatccgaat aaacgtcccg ccatagatac ggttcgaacg ggagaatgta aataagattc
     3601 tcgggtccgg cggcgcgtgg caggggcatc ctgcccgggg ttgcggaaca cgagctggaa
     3661 gcccgaagag cggagggcag gatcgagaca aattgcgcgc gggcgtcggc gggaaacgcg
     3721 gcgggcatgg ctgtttgctt ttcggcgggg gcgttggcag agtggtgttt tgcgttgagc
     3781 cggaaagcga ttgcggatgg ccgccgagca gaaccaacct cccagcctgg ccgagatcgg
     3841 cgaacgcgcg tggctgcggc gcgtgcggcg ctgggtcgag cgccagccgg acgcaatgcg
     3901 cgaacgactc gtcgtcggcg tcggcgacga tgcggcggtg ctcaagctcg gcgacccgcg
     3961 ccttgtcgtc accaccgatg cgctcatcga aggggttcat ttccgcccgg aatggaccgc
     4021 gccgcgcgac ctgggctgga aggcgatggc ggccaacctg tcggatctgg ccgcgatggc
     4081 cgctcgtccg gtggccgcgt tcctgagcct gagccttcct ggcgaatcgc tcacgcatga
     4141 tgtggaagcg ctgtttcgcg gaatgctcga ggccgggcga cgttggggtt gtccgctggc
     4201 ggggggcgat ctgacgcgcg cgccgcaatg gtgcctggcg ctgacgctgc tgggtgagcc
     4261 gatgcggcgc gggcgcgtgg cgcggcgcta taccgcccga ccgggcgacc ggctctatgt
     4321 gaccggcgcg ccgggggaat cgggcgccgg cctggaggct ctacggcgcg gattgaaggg
     4381 gaaggcgccg gccgggctga tccggcgcca ccagcggccg acgccgcgcc tggccgaggc
     4441 cgaggtcctg attcattgct gccgccggct gaccatgctc gacgtaagcg acgggatctg
     4501 gaacgatgcg tcgcaactgg ccgaggcatc cggcgccggc atcgacctgg aggcgagccg
     4561 gctgccggtc agccggccgc tgcgccggct gggcgcggcg ctgaacgtgg acccgctgga
     4621 ctgggtattg ttcggcggcg aggattatga actgctcttc gccgccgggg aatccgaaga
     4681 acggattgaa cgggcattcg gggcggccgg actcgctacg cccgtcagcc ggatcggcgt
     4741 cgttcgcaag ggacgcggtg tgcgtctgct cgacgaacgc ggccgtccga tcccgcgggc
     4801 ggaccggacg tttcagcact tccaggagag cgagccatgg cgcaacccga gcgcgaaatc
     4861 ctgatcgagc gcaaatacct gcggatggtg cggcaggggg ggtgggagta tgtggaacgg
     4921 gtcgggacgc agggcgtcgt cgtcattgtt cccgtcaccg acgacaacga actggttctg
     4981 accgagcagt atcgcgtgcc gttgggccag cgggtgatcg agctgccggc gggcctggcc
     5041 gccgacggcg ggcgcgatgg cgaaacactg gaggaggccg cccgccgcga attgatcgag
     5101 gagactggct acgaggcgcg ccacctagaa cgcgtggggg ccggccccac gtcgtcgggg
     5161 ctgaccaacg aagtggtcgc tttctaccgc gcgcgcgggc tgcgcaaggt cggccggggg
     5221 ggcggcgacg cgtccgaagc gattgaagtt cacaccgtgc cgctcgacca gattgtggac
     5281 tgggtgaaac gaaaggcggc cgaagaccgg ctgattgaag tcaacgtcta cgccgggatt
     5341 ttcttcgccc gcggcttcga gaccgtcgcc gactgcgaca cgaccgagga gagaccctga
     5401 tggaccggcc gtttattcac ggcgactttc tgctgcagta cgacgacgcg cggcgcctgt
     5461 atcacgaaca cgccgccgcg ctgccgatcg tggattacca ctgccatctg ccgcccgaac
     5521 cgatcgccgc ggacgcgcgc tttgagacga tcaccgaggc gtggctgtat ggcgaccact
     5581 acaaatggcg cgcgatgcgg gccaacggcg tcgatgagcg cttctgcacg ggcgacgcct
     5641 cggactggga aaaattccag aagtgggcgg agaccgtccc ctgctgcctg cgcaatccgc
     5701 tgtatcattg gacgcacctg gagctggcgc gctacttcgg cgtgaccgag ctgctcgacg
     5761 gcgcgagcgc ccggccgatc tatgagcggt gcaacgccga gctggcgcaa ccggagaact
     5821 cctgccgcgg gctgatccgc cgcgccaatg tcgttctggt ctgcacgacg gacgatccgc
     5881 tctcggatct ggaggcgcac cgccgcctgg cggaggatgc gtcgtttccc gttcgcgtcc
     5941 tgccgacatg gcggcccgat cgcgcgatgg ccgtggatga tcccgccgcc ttcaacgagt
     6001 gggtcgaccg gctggccgag ctgacgggca tggacatcgg ggatttcgag acgttcctgg
     6061 cggcccttga gcggcggcat cagttcttcc acgaaaacgg gtgccggctg tcggatcacg
     6121 ggctggagga accgtatgcc gaggactaca cggctggtga agtcgcggcg gcgttcgcac
     6181 gcgttcgcgg cggccaggcg ctcgatgcgg cggcgcggct caaactgaag tcggcgatgc
     6241 tgtatgaatt cggccggatg gatcatgccc gcgactgggg ccagcagttt cattttggcg
     6301 cgctgcgcaa cgtcaactcg cggatgatgg cgcggctggg ccgcgatgcc ggcttcgaca
     6361 cgatcggcga cttcgaccac gcccgcccgc tggcacggct gctcgaccga ctggagcgcg
     6421 aggaccggct ggcgcgcacg gtgctttaca acttgaaccc cgccgacaac tacctgttcg
     6481 ccgcgatgat cggcaacttc caggacggcc ggacgccggg caagatccag ttcggcagcg
     6541 cgtggtggtt tctcgatcag cacgacggga ttgaacttca gctcgaggcg ctctcgcagt
     6601 gcggcctgct aagccggttc atcggcatgc tcaccgactc gcgctcgttc ctctcgtatc
     6661 cacgccacga atatttccgc cggatcctgt gcaatatgct ggggcgcgac atggaacgcg
     6721 ggctgattcc gcgcgatgac gcgctggtgg gccggctgat ccgcgacgtc tgttaccaca
     6781 acgtggtggc ctggctgggg ctggatggcc aggcgctggc ggcggcggac cagggcgggc
     6841 cgaactggag cgcgtgaggc gtcgggggat tgtcgggcga aaaccgatag cgatttcgag
     6901 tcgtccgcgg ccggcgtggc atgaaccgcg cttaccgatg cgcggacgcc acggcctgct
     6961 ggagccggtg gacgttctcg gccggatcgc cgccaaagac ggcgcggatc atcgccacgc
     7021 cggtcgcgcc ggcggcgagg acctgttgaa tgcgttcggc gcggatgccc cccagggcga
     7081 tgatgggtgt cgacgtgcgg cggcgcaggc cggccagggg ttccaggccg atgggcgtcg
     7141 cggccggttt ggactcggtt gagaagaccg ggctcagcac gacgtagtcc gcgccgcgcg
     7201 cgatggcctg gagcgcttcg tcttcggaat gggcggaata gccgatggcg aggccgtcgc
     7261 ccagatggtc gcgcagttga tcgggggccg ggtcgccggc gccgatgtgg agcgcatcgg
     7321 gcgcgagcag gttgatcaga ctgaagtggc ggttcagaat gtaaagcgcg ccggcttggc
     7381 gcgccgcctc gcgcaacggc caggccaccg gcaacagttc ccggtcgctc agctcccgat
     7441 cgcgcaccat cagcgcgggc agaccggcgg ccagcgccgc ggcggccagc ggttgcaagg
     7501 ccggccacgg tttccagccg ccgtcggcga tgccgatcag gcggagccgt tccgcggcga
     7561 cgcgggaggc aggtggttgc ggcgacatgg gttcgatcat tccacaggcc ggcggcggcc
     7621 gccatcgtgg tttttgcccg gcggtgcaat agaagcctga gtgtgcgtgt cttattgatt
     7681 tcaggaggtc accgggagcg ggtgagggct tcaggccccg gccgcttcag ggtcggaaag
     7741 gcatcccacc ccccgtgccc aaacgcgaat ccgtgataga cgggccgccg gcgcgccccg
     7801 ccttcgaatc gctctacgcg cagtggagcg aaccggtcta ccgttatctg gcgcggctgt
     7861 tgggagccgg gcccatcgtg gacgatctgt tccaggacac ctggatgaag gcgatcgagc
     7921 accgcgggca gttgcgcgat gccgaacgct tcggtccgtg gatttttcgt atcgcgcgca
     7981 atctggcttt caaccagcgg cggcgggggc ggcgccgaac ccaggtctac gccgtcagca
     8041 gcctgtcggt cgccgagggt caggagcccg agagcctgat tgagagcgac ggtgaacacg
     8101 aggcgccgac gccgcgcgat gccgccatcg acggccagcg ccgccaactg atcgaagcga
     8161 tcatggagga actgaccgac gacgcgcgcg agatgatcca cctgcgctac tatgagcaac
     8221 tgacgctggc cgaggtggcc caggtgatgg acgtgccgct ggggacggtc tgcacgaagg
     8281 ttcatcgctc gctgcggacg attcgccgcc gcctcgaacg ccaggggcac aggagtctgg
     8341 agacgatttg agggccaacg gaacactcgc aacgacggga tcaggaggcg gggtttcgag
     8401 cgccgagaac gagggacgag agccgaattc cggggcgggc atttgggagc gatcacgagg
     8461 gacgagggac gagagacgaa cttccttggg ggatgagatg agtcaggatt gcacccagct
     8521 tcaggcgcaa ctgctggacg acccgaatgg gccgctggac gagaccgcgc gccgacacct
     8581 ggcgacctgc ccggtgtgcc gggaggtcgc gcgcgacgcg cgcgcgatcg gcggtctgct
     8641 ggagcagccc ggccgcgacg ctccgccgct gctgccggcc gaattgcggg aagcagtggg
     8701 gcggcggatt caggcgcgtc tggccgccgg ggccagcccc cgcgcgaatg ccgcgcgtcc
     8761 gccggcgacg gttcgcacgc tcgcgcgcgc cgccgccgcc ccgcggtttc ccaaatgggg
     8821 ggcgctggcg gccgcggccc tcgccctggt gatcctcggc tgggccttgt ggcatccggc
     8881 cgcgtccgca cgggtcggtc aactggattt catgagcggg gaattcaggc tgagggccgc
     8941 cgcgggcggc gagcgggcgc cgggcgccct cggccccgga gatcgcctcc acacggctca
     9001 ggactgcgtc ggctggatca gtctgggcga cgaccgggag gtgcgcgtcg ccgtcgcgcc
     9061 cgacacccgg ctcagcatca tcgatcggac ccgtctggaa ctggagcagg gacaagtgtg
     9121 gcttggagtc gagcccggcg gcgaaggctt tcaggtcgcc acggcgcagg ccgtcgtccg
     9181 cgtcaccggc acgcggtttg gcgtcgttgt ggcgggggga gcgacgcagg tggacgtgac
     9241 ggaaggaacg gtgcaggtgg cgtcgccgac gggcagggtt gcacggctgg cggcggggga
     9301 gaccctgtcg gcaacggccg cgggcgagct gaccgcgccg ggtccccgcg cggaggggcg
     9361 cgagcatccc cggtgggtga cgacggccgc cgcgctggag caggcccacg ccaaggcccg
     9421 ctacctgccg agtgtcggcg ccgcgcggga gtagaattcg agagtcggga acgagggacg
     9481 atttgagtga ggacggcggg acggaaatcg gcgcgggggt gattgacgga tcggggggcg
     9541 tgccccgata cttgatctaa gatccagacg ccggtccgaa accgaggggt ccacgacccg
     9601 gacgggaggt ccaacggtag acagtacgag gcgtgggccg ctcccctgac ggcccggccc
     9661 gagaggaacg gatgccgaga ccgaacaggc gctccctgcc cgcgcgaggg cgaggcggat
     9721 tgcaccgttt tgccacgggg tcgaggtgcg ggctgacgct gattgagctc ctcgtaacgg
     9781 cggcgatcgt cggcattctg gcggggattg cgatggcgaa ttttcagcga gcccaggttc
     9841 aggccagagt cagcgccggc aagagccaat tgcggacgct cgccggcgcc gtcgagcgct
     9901 actgcgtcga ctacgggacc tatccgacgc ccgcgccgtc gttcccggac gatccgttcg
     9961 gggtggtggc ggcgacggcg ctggcgagtc tgacgacgcc ggtggcctac atcgccgccg
    10021 atgcgctgcg cgacccgttc ggcacgctgc ggctgcaagc cggtccgccc agtttcggcg
    10081 accggccgcg gctgacatcg gccgatccgt tccggccgcc gacgccaggc ttcaacatga
    10141 ttcagtcgct gctttttttt cactatccac gattttccta cctcatcgat caaccggcga
    10201 tgaatgtgca cggctatgcc tcggtcagcg tcggaccgga ccgcgccgac tccttcatcg
    10261 tctacctgcc cttcccttcg catctcccag ccggagcctc ccattacggc gtcaacaccg
    10321 ccgccgatac ggtctatgat ccgaccaatg gaacggtcag cgggggcgac atggcctggt
    10381 ttggcggcca gctctcgcgc ggcgggctga tcggcggagc gccgtgagga ttttccgggg
    10441 cgcccggttg gttttcatcc cccgcggcgc gagcggtggg atttggtatc ggttcctccg
    10501 gctgctgcgc ggggtgctgc tggccggcgc gctgggaatc ggcccggccg gcgcggccga
    10561 cgagttcgtc tggcggccgg attcgccgat caaggatatt gcgtcgcccg gcctggtcgc
    10621 gtcgcacgcc gccgcggccg actttacggg cgacggcctg accgatctgg ccgtgacgta
    10681 tgccttcagc gagcaggtgc ggctgtatcg cggcgacggc gaggggggct tcagcgaata
    10741 cggccagctc gaagtcggga ttcatgaggg cgccggacgc gacctgccgc gccagatcgt
    10801 gatcgaggat ttcgacgccg acggatttct ggatctggcg attctctgct cgggcaaccc
    10861 ggatgttcat gcgccgccgt cgctggggat ctgccacggc gccccgcgtg gactgtttga
    10921 gccgttcgag ccgatcgagt tgctggcgga gaacaccgcg gccgattcct ttccgctcgt
    10981 gctgcgcggc gcgcggctcg ccggcgacga cgcgcccagc ctgctcgtcg ggcatttcga
    11041 ttcggcccag ttcaccgtga tccgtcacct gcgcggccgc cagtggcgca agccggtcac
    11101 gttccaggtc gatgcgcggg gagcgggtcc ggccgatctg gaaccggtcg atctggactt
    11161 cgatggcttg accgatctga tcgtgctcaa ccggcgcgac attcagttct ggcagggcga
    11221 cgccgacggc cgattcggct accggggccg attggaggcg gagttcgcca acgccgcctt
    11281 tacggcaatg gcgccggccg acttcgaccg caacggcgcc tgggacctgg ttgttctcga
    11341 cggcgcacac agccggctgc tgctctatct gaatctggct ttcgatgccg atttcgaacg
    11401 gcggctcgtt tacccgttca cgggcgacat cgcctcgacc aaccatcgcg aggggccgat
    11461 cgatctggtg ctccacgacg cgaaccagga cggctgggac gacgtggccg tggcgtttct
    11521 gcaatcgggg ggcgggagcc ggctgctggg gcgcgccgac ggggcgccgg acccactgcg
    11581 cctgccgccg ctggatttcg gcaccggtcc cggcccgcgg gcgatcctga gcgcgcacat
    11641 cgacgcggac ggcctgcccg atctggtgac ggtcaacgaa ggggcggcgg gcgaagcgca
    11701 gcacgatatc accgtcgatc ttgccatcca tggggtgggg gggccggcgc cgcagacggg
    11761 cctcaatgcg caggcgcccg gccgcgggat ctcgccgtat ctggatcggc cggtcgggct
    11821 ggcctgggat gaggaacgcg acgcgctctg gctgatggat cggcggcggc ggcgcctggt
    11881 gcgcctggac gacgacggcg agctccagtc cgagctgccg ctggactccg ccaatcaggc
    11941 cggtccgctc gatccgatgg acctgaccgt cgaccccgcg ggcgagctgt ggctcgccga
    12001 ccggctcggc gcgcaggtgg tgcatttgcg cgcgcacggc gagcgggcgg ggggcttttc
    12061 cacgctggcc gccgggttga cccgcccctc gggcatcgcc cacgccgcct cgcctttccg
    12121 gctttatgtg tcggatgcgg ccagccgtcg gctggtcgcg ttcgatgccg ccggcgccgt
    12181 gatccagacc tgggaacgcg cgccgatgcg ggatctggcc tgggacgcgg aacacggcat
    12241 tctgtggggc gtgcccgagg ccgaaccgca ggtgttgatc aatcttggca tcgcctccga
    12301 tgacgacgac gatgacgata agaccgacca accggcgcag cttgcgctgc acgaagtcgc
    12361 gccgctgctc aagcaggaac ggatccgtgc gatcgccttc ggcgaggagg gcccctggat
    12421 cctgaccgaa cgcggcgcgc tgatcgagac cgacgacgac ggcgaactga agtcggtccg
    12481 cgaattgcgg ctgctgcgcg acatcgcggc ggccgccccc gccgacgcgg gcggcctgtg
    12541 gctgattgat tcgggattgc tggccacgct cgtccgcatc gacgccggcg gcgcggtcca
    12601 gtcgcgccac caactcaatc atcccgcgat gagcgacccg atccgtccgc tcgggctggc
    12661 gcggcggaac gagcggctgt acctgctgga tgggcgcaac ggccaggtcc ggccggtgga
    12721 gggcgatcgg ctcgacgcgc cgcccgtcgc cctgctgccg atgccgccgc ggccgagcgg
    12781 tctgtgttat gacgaatcga cggattcgtt ttactccgtc gcccccggcc ggctgatcca
    12841 gctccagccc ctgggcccgg aggacggtgc ggcgctggcc agcgacgccg tcgtgagttg
    12901 gtatccgctg ccggcgacgc atcccccctc atcgctgact cgcgggcgcg agccgggcga
    12961 gctgatgatc tacagcgcgg atcggccctc ggtgatgttc cttgacctgc aagcgccggg
    13021 ggtgcggctc atcacgccgc tgctgctcaa tctggtcgac ttcgttccac ggatcgtcct
    13081 ggccgaggag gctgccgctg gcgtgtggcg cctggtcggc tccggcgatg agcccgtcca
    13141 ggagatccgg atccccaccg tcaccagcgc cttggagtca ggctggacgc ggtaccggta
    13201 gcgcacggcc gcgtgcccat ccggcgccga tcgggcgacc ggatggtggg cctgcacctc
    13261 tctcaatgtt catagggaca agcgatgaat ttcgctggag gcgcgggcga aatctcactg
    13321 ggagaatacc gcatggcgcc caccggaggg tccggtagcg gaagggccac gggaaaccgg
    13381 aatccggcgg ggggcggccc gttcggcgcg atgcgtgcgt cgtgcaccct tgaagtcggg
    13441 ggcccccctc gcccccctcc atcacttgca agggagtgcg accatggtaa cgcgattcaa
    13501 gaaagcgacg acgtttctga tctcggtcgg cctggcgctg ggaagcgtgg cttacgccca
    13561 ggagcagcag aagccgatgg atcagaagga gcagaagcag gaaaaggtgc tgaagagcga
    13621 caccgaatcg ggcgtgctga tctatgagcg ccaggtgctc gcgaccggcg acggaacgcg
    13681 gctggaaacg acgatcaagc tgcccgaagg gatcgcgccg aaggaagatg cgcggattcc
    13741 cgcggccgag gaagccatcc gtaatgtgat cagcacggcg gtcgacgcgg ccctggccgg
    13801 cgataccgac aagctgatcg aacagctcag cgggcccgat cgcgagcggt tcgaaagcca
    13861 gaccgacgcc gactggaatc cgctgaccaa gaccaccggc tttctgaata agacctggaa
    13921 agagagctat ggcctggcca ccgccaacgt gaccaaggac gacttgagcg caacctacat
    13981 ggcggtgcgg atcggcgaga tcgaggacgg cgagaagctg accgaatggc cgctggagcc
    14041 gacgccgacc acgaacctga tgcagaagaa gggcgccggg acggcggatt acatggagcc
    14101 gggccgggac gtggcggtgg tggtgctgcc gtcggcgaac ggtctgccgg agctgacggt
    14161 gtcgatgacg catgaagcgc ccgatttctg gaagatcgac gtgccggacg acatgacgct
    14221 gaaggctctg caggagagtc tgacgcttca tctgaaccac ttgaatgaga acgcgcgcag
    14281 ctggccggcc gatgaggacg agaccgaacg gctgatcacc cgtcacgtgc tgatggggat
    14341 ctacggggtc gacgccgaaa tggacgatgt tgaagaagcc cagtcgacga cgcccgagcc
    14401 gcgcggatag tcgcggaccg cgggagttgg caagcaacag ccggtcggcg tcagagggcg
    14461 ccggccggtg ctgcgtcggg gcagggacga gagacgattc aggggatcaa tccaggccgg
    14521 cttcggccat ttcaacggcg cgcagcatcg cgcgggcctt gttgaccgtc tcctgatatt
    14581 ccgtggcggg gacgctgtcg gcgacaatcc cggcgccggc ctgaatatag acgcggcgcc
    14641 ccttgatgac ggcggtgcga atcgtgatgc aggtatcgag gttgccgtcg aaactgatgt
    14701 agccgacgca accggcatac ggtccgcgcc gcacgttttc cagttcgtcg atgatctcca
    14761 ttgcgcggat cttgggggcg ccgctgaccg tgccggcggg aaacgtggcg gcgagggcgt
    14821 cgaaggcgtc gctcgtcggc cgcagccgtc cgcgcacgtt gctgacgatg tgcatgacgt
    14881 ggctgtagcg ttcaattgcc atcaaatcgt cgacgtggac cgagccgggg cagccgacgc
    14941 gtccgacgtc gttgcgcccc aggtcgacga gcatgatgtg ctcggcgagt tccttggggt
    15001 cggccagcag atcctgttcg tgctggcggt cctcggcggg ggtggcgccg cgcgggcgcg
    15061 tgccggcgat cgggcgcacg atcacctccc catggttgac ctggacgagg atttcggggc
    15121 tggagccggc cagtttcaga tcgcccagtt gcagatagaa gttatagggc gagggattga
    15181 tcgcgcgcag cgcgcggtaa atgtcgaagg ggtcgccgtg atagggcttg ctgaagcgct
    15241 ggctgaggac gacctggaag acgtcgccgg cgcggatgta ctccttgacg cgctcgacgg
    15301 ccgccatgaa ttcatcctgg ctgaaattgg agacgagctc gctgacgggg gactcgacgg
    15361 cgggggaaat ggcggcgtcg accggctggt tgcgttcgcc cttttcgatg cggaggcgct
    15421 cggccatcag ctcgatctta tggatcgcgg cgtcgtaggc gcgccgcggg tcgtcggtca
    15481 cgtgcgcgtt ggccagcagg atcatgcgat ggcggacgtg atcgaaaatc gccagcttgt
    15541 cgctgatcat gaaataggcg tcgggcagct tgaggtcgtc gggattggcg tcggggagct
    15601 tttcgaagtg ccggacgaga tcgtaggcca tgtagccgac ggcgccgccg atgaagggtg
    15661 gaagctgggg gtcggcggcg gggcgatagc gctgcatgaa ctcgcggagg aattcgagcg
    15721 ggggctgcga ctgttcgagc ggctgttcgt cgccgtggta gcggatgtcg atttcgtgac
    15781 cgcggcagcg gatgatgatt tcggggttgc agccgatgaa cgaataacgg ccgacgtttt
    15841 cgccctgctc gacgctttcg agcaggaagg cgtgctcgct gtcggccaac ttgcggaaga
    15901 cggagacggg ggtctccatg tcggcgaggc gttcggccca gacggggatc aggttggcct
    15961 cgcggctgcg ctggagaaac gtttcgaaat cgggggagac tttgagcatg cggtctttca
    16021 ccggccgggg gtgcggggca tcttcagcgc cggcgacatg aagaccgtca aaggattgca
    16081 cgcgcccgcg cgcgggatca agcgagttat cgtttgaatt ggccgcggag cggagggact
    16141 gcacaaatcg ggtaccggat cggatcatca cttgatgcgc aatccggtca ggaaaaccat
    16201 cagcacggat tgacgacgcc tactcgcgca ggaggcgggc gaactggtct tcgtcgagaa
    16261 ccgggtgggc ggcgaacggg cggtaaagcg ggtcgccgat gagcgtcatc atccaggaga
    16321 cggaggggag cgtgagggcg tagacctcgg cgagcgagcg acggccggcc agcaggtggg
    16381 cgaagaactc agtcggccgg gggaaggcgg tcagatatgg ttcctcgacg gggccgagcg
    16441 tggcggcgac gccgtcgcgc agcagggcgg ggcaccagtt gcgcgcattg tcgcgccgca
    16501 gcgtcatcgc ctcggaactg gcgatgtggt agccaacggc gccggggaca aactcgaacg
    16561 catcgacgta gttggccagg ctgtaccacc cgcagtacag cgcggcggcg ggggcggcgc
    16621 ccggttggaa gagcgtttcg tcgtcgtcga ggatgacggt cagcgacgtt tcgcggtcga
    16681 gaaaccgggc cagccggcgc aggttttcgt catagtcgcc gtagccgctt tcgctg
//
LOCUS       DAOPCO010000055        16531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3555_length_16531_cov_5.415210, whole genome shotgun sequence.
ACCESSION   DAOPCO010000055 DAOPCO010000000
VERSION     DAOPCO010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_16531_cov_5.415210"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLS90_06040"
     CDS             complement(<1..184)
                     /locus_tag="PLS90_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPK01999.1"
                     /translation="MPDWRFLFDPDHRTVALHLLSALVAGGLIGFERSFHGRPAGFRT
                     HALVCLASSLLMLVTYF"
     gene            complement(335..1282)
                     /gene="era"
                     /locus_tag="PLS90_06045"
     CDS             complement(335..1282)
                     /gene="era"
                     /locus_tag="PLS90_06045"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPK02000.1"
                     /translation="MNHPDPPHSPSPPPEHPPRRVGFVGLAGVPNVGKSTLVNRLVGA
                     KVSIVSDRPQTTRERVCGIYTDDRMQAVLVDVPGILEPADAFNEHLVEWAAFGLRHCD
                     LILHLRDARRPPRGAVTPPEEEPVLAMLRRTNRPVWVVWNKIDRVPGNFFPAEAPDFN
                     YQRAFGIAARTGRGLPLLLDAIADALPEGPLLYEPDQVSDRDLRFMASEMVREKLFRY
                     LGQEIPYATATQTEIFDENRPGKPLIRVLIITEREAHKSIIIGKGGAMLKKIGENARK
                     DIERLYGQPVFLQLWVKVRPKWRRSEAQLGQLGLKPPRG"
     gene            complement(1279..1779)
                     /gene="ybeY"
                     /locus_tag="PLS90_06050"
     CDS             complement(1279..1779)
                     /gene="ybeY"
                     /locus_tag="PLS90_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPK02001.1"
                     /translation="MTPRATPRIVWSLRGRLGRHAQDLGPLGAELLGRLARRAGLDRR
                     HELSLVLCDGPTIRRLNRRWRGHDQPTDVLSFPLHPGDPRRSGATGAVGDIVVALPVA
                     RAAARAEGIDPGRHLARLLAHGLLHLLGYEHDTPARRRSMAAAEDDLLRDEGVEGLIR
                     RSARMP"
     gene            complement(1776..3032)
                     /locus_tag="PLS90_06055"
     CDS             complement(1776..3032)
                     /locus_tag="PLS90_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK02002.1"
                     /translation="MHILSLRWLPGRPRHDRPRPLSGLLLIALLAAACSTKSPEEKLE
                     EANQWAREGDVLNAQFAYEEIIEQHPDTKAAIDARIGLAMCYLNDGDLTRAREQLDDY
                     VDAVGLETEEGLGARVLKIRSYGQERQFEQAIAEAIATSNSLPMEQPGLLFELSQLYA
                     ADEQYDQALELLQYLLSREDQRPEEQMFPLERMAALYRQMGQPEKSVAVYEEYLEQHP
                     DSPQRPEILFAIGDYQLQAGNEEAAEASFNASEALYHQQIDEALGAEEKSRLYLRMAR
                     VQELRGQVQAAIDSLRTVIDKYPTSSVRSEAQRRLATLLFSRGRFDQVLAVMEQALAE
                     SPVPEDALVIEREMERLKQFIADGVTSQTLFASMASTATLAQAGLTTASLELTEGEPP
                     APAETPSPSTEAAETPATGTLTSTAP"
     gene            complement(3406..3870)
                     /locus_tag="PLS90_06060"
     CDS             complement(3406..3870)
                     /locus_tag="PLS90_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02003.1"
                     /translation="MLTIRHATFKDSSALADLHLRQLGQPALSDGDANHYQTAFATFL
                     DRALSGGNWTVWVAGDEQQLLLALYLVMLRRLPHPTRRDAPVGILTQLDALPAALDES
                     VMLPVLRNLINWCRSQGLGMIHISPWLPVLPFYQKLGFQRCDRALLLELERE"
     gene            complement(4074..5264)
                     /gene="aroC"
                     /locus_tag="PLS90_06065"
     CDS             complement(4074..5264)
                     /gene="aroC"
                     /locus_tag="PLS90_06065"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPK02004.1"
                     /translation="MLRYMTSGESHGPQLTAIVSGLPAGLPIRAEQVDRELKRRQGGY
                     GRGGRMTIESDQVRFVSGVRHGFTLGSPVTMVVENRDWRNWTEIMSPDPQPEVQGKRA
                     ITRPRPGHADLPGVQKFGHDDARNVLERASARATTTQVAVGALCKALLAEFDIAVLSH
                     VVEMERIKADVSGLALREIAERAEASDVRVADPAVEAAMRALIDECKKEGDTVGGIFE
                     VIVTGLPAGLGNVMNWDEKLDGRIGQVMMSMQAIKGVEIGLGFEAARRRGSQVHDAIE
                     HSPDPAAWPEGHGPSGGFFRRTNGAGGLEGGMTNGEPLVVRAAMKPISTLMRPIGSVD
                     VKTKEPFDSSKERSDVCALPAAAVVGEALVAYAVAGAFLEKFAGDSLAEVRRNYQGYV
                     DSLG"
     gene            complement(5436..6524)
                     /locus_tag="PLS90_06070"
     CDS             complement(5436..6524)
                     /locus_tag="PLS90_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02005.1"
                     /translation="MRVLHVIDSLRLGGAQTYLLSLLRELGRLDVDCAVAALWGPQSL
                     SSAFAEAGFAPRELARSRFDARLVPRLRAHIRRLRPDVVHAHLIPSVFLTENFRNGLG
                     IERLVTHVHATPGRARGYQRPLERRCFRNADLVVACSRAAAARLPRADRVEVVVNGVD
                     ADRFRPPTAVERAAVRRHWGIADEAIVVGTVGRLAPVKNQHLILQAAARLAAAEPRLR
                     VLLVGEGPCAAGLRAEAHALGLGERVIFTGLQRDVAPLLGAMDIFMLPSAHEGLPLGL
                     IEAMACARPVIASRLPGIEEVDGDSGSLVLVQPEPDDLAQALGELIQSEAARSRRGEA
                     ARRRVQAAFSVASSARRLASCYRDLRAA"
     gene            6789..8948
                     /locus_tag="PLS90_06075"
     CDS             6789..8948
                     /locus_tag="PLS90_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02006.1"
                     /translation="MIRWTSPGLVRLFSIGILCAAFLACDRGRPVEGFYLSTIGFEPL
                     DPIEIATTNQGNLSMGSIRSPATSSFGSPNSKIEIRAGSRGFVVREPPDKKGFARLWE
                     VIVARAKGETLPDQFPLLQLKASAERPGDWDLVGLRVAGNFQVLPNPVPSALHRVEDP
                     QVREYFHLQDELGRDGEALALAEKLLAAHPNDPWIHALQLDALLAAKRIDEAEAFVAA
                     SRDELLKHTESGFAHLVREAERRVVGLRAAAEGRNAAALPGVVTFTMQWPDDLLPYEA
                     VMDGLERALECDVMVIQGRGLRSDPRGPVPNFLHFQVMAKIARAEAAGRLLLGRRDDA
                     LRLQAGACWLGQMMQRDGTLIQCLVGTAVRRIGLEGLQTYAFNACETDGDFRELWRHL
                     ERFAAAPVPHAEAELFAGEGWLGLKLDELNAGPTPGEAAKRVLVTKAQLELLRMATAA
                     RHRLVTQGDFPNPLSRPFSPLLEQIPDDPFTRPETVANDAKTSTSAPLRHAVRPGDET
                     FVCWSVGPDGLDDAAGIPYDPTNGTLSAGDLFVVVPREREIPFPREGVRVDSVQEFME
                     LFPNGLPVDPFADTKTRRLSLIDRRPVTVFSHGPDTDERVGPPDPRIWTWEPESREWL
                     PEPQWTAIQEARRGFQPAAAGMLPGSPGGAAPGFQPGAGAPIQPGARPPAMPADGATP
                     AAAPLSHPEAPYDPSNGTISDGDLYWEMMGREGAGGR"
     gene            9570..11804
                     /locus_tag="PLS90_06080"
     CDS             9570..11804
                     /locus_tag="PLS90_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02007.1"
                     /translation="MARRRSEAAAPAAAPFGRAALPLAAALRLGSQFPLRLELVLTES
                     AAAGKLGPDEDPFVVLSAHGGRSRVYLGRVLAGEANLLSHVAVKLAPRRGTPSGLEGQ
                     SLTEGQRLERWEKEWQHYAALAESEEAISPILFVTPGEALAGSAARPITWPPLVYCPI
                     TRTLLQPRSREGGRLTLCRDDATLVEHGLPPHGERYAFLWDAEAALQGQEPRFYQVPV
                     PGEPVLGPAQPFSQCQREMAELLADPAGLIDLTTPDGLARLADLTQAFPGLTNPEIRE
                     ELLRRDPTELFIPWNLDEAPALVMQLNTFHCDEFSDLLGGIEEKEFVARYQGPVAAAG
                     QRLRLARAPVGERFNRRLLFSGDGSGLDALEIFRLKWSLVGQIVRSTLEFQRRTGSPH
                     LRLAPEHVMIHIPESGGYLPGCWTFQTRLISLGAPTLEIPGQTDLPPIHVPPVGSDPL
                     YQAEIIRNSSFGLTQRGEFQLTGLEPSGERTVIVAQLHHEGLGLRWLSPKDHVHVSLG
                     RHLVGEAAIDFLSRRDENKEYSGRTLYLRSLPLDLDKTMQAELQKLRGIRVPNAQFRL
                     LPTLHVPCDLYSLGMLLYRVLLVNDHQGIGDVALALDELRQDLASLADVEVDAGKEAF
                     FWDALLSGHRRPETAEVFDRRQIFYHEHQRSDDRPNAIPRRLWHEAMVIGLQLVTQFE
                     GFSFAAHHGDFDESYPSERLEPVVRRVEELGRRLDAALFSFTARNAEVRQALELVTQE
                     IRVG"
     gene            11877..13754
                     /locus_tag="PLS90_06085"
     CDS             11877..13754
                     /locus_tag="PLS90_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPK02008.1"
                     /translation="MIAGPLDGLSFEGRKASVRIGRSDGTGPGDVRNDLILQRDSYAS
                     SFHAEITHQEGSWQVRDLNSTNGTWVGARKLERGEQVAVGNGEVFVIGNTAIQLTEKP
                     AGLPAIDASISAILSPASQTMINRARTLARELNQPWVGVAAVFRVLAQSDAPPLPDFF
                     KALGVDPAAAEKAVLDFEYWKDELDWIGKEVMRGRRPAGQIRNVVPTPRLQLVLTLAD
                     RVRENQNEPNVEPQHVLAAMMVEPGNIVLRALAEAGHATAALRQATENFMKPPPEKKA
                     EPEPLAEPEPEPAETTARISLPGFKGTPLRKPVDPETWLLARQAMDRIEVVQNDYSMA
                     DPAVRFDALCTVIRDAVQKAPGPKRALLIEQLHYLFPVLEEAVLPPPPSLFHEQTPAS
                     LQGGEITDHELEGTGDRARMRPSDDVSATLKADRSQPQRETTLDTDYMLQQVFATEKL
                     APLGASSESDSQFLRLMQLLYTFALDMERLAKGMVQSLQGGGGGESRYFLPFTMQDLK
                     MMVQKILEQKADPETVVDIHQYLSDLSHWLIALIAAHQKGTVQWYGELWERISPAGVR
                     NETKVSSLNKVLGQSKADLWETYERLARDLKPEIVEDQFKEKLSQLVTEEFRRLSSRR
                     E"
     gene            complement(13767..15323)
                     /locus_tag="PLS90_06090"
     CDS             complement(13767..15323)
                     /locus_tag="PLS90_06090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02009.1"
                     /translation="MTRADAPKPDPQAPKCCLLDAHGQILSASAALAALLGIRVQDSA
                     RHSLLEFIHPEDRALHTPFKTTSDYAFRQMLRIRRHDGSYGYMGLSGMRDPDSRKLFV
                     HLTDLSGSARGMSYDFDVAQIVRQKDEELLDLQERFRQAQKLEAVGRLAGGVAHDFNN
                     LLTVILGQAEMTLLGLPEDSPIRPDIEEIRRAGEKASALTQQLLAFSRKKMLRPRVLN
                     LNAIVRNLDKMLRRLIGEDIEILTRLDPHLRNVVFDPTQAEQIIMNLVVNARDAMPNG
                     GTLTIETANIFLSDDFAGGAIFSVKSGPHVLLSISDTGIGMEEETLRHAFEPFFTTKD
                     RSKGTGLGLSTVYGIIKQSGGNIWLYSEPGKGTTVKIYLPRADQPESIADDPEPRPIR
                     HGFGQEILLVEDDDKVRATLTQMLRGEKFTIHEAGSGDEAIRLFERQRDKIALLLVDL
                     VMPKMSGPEVARRLAAIKPDIRTIFMSGYSEPTALLNRSLPPGAHFLEKPITRIDLLS
                     SVHKALQQAP"
     gene            15493..16122
                     /locus_tag="PLS90_06095"
     CDS             15493..16122
                     /locus_tag="PLS90_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02010.1"
                     /translation="MQLLMASPVPTPSSRAFTLIELLIVVAIIAILAAIAVPNFLEAQ
                     VRARISQTHAGLRTTAVALEAWRVDYNSYPSRSPSQFQILEALIPLSTPVPYVAEIPS
                     DPFVANLPASYPDGINWNLAYKNHLGEWTFPRPFDYQPRLPNALWASVTSHPESARWA
                     LRSAGPDIVPTWLGYGAPAYDPTNGTVSAGDIYFTGPGIGLDQPAVPLL"
     gene            complement(16192..>16531)
                     /locus_tag="PLS90_06100"
     CDS             complement(16192..>16531)
                     /locus_tag="PLS90_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK02011.1"
                     /translation="IVVVLSGDITQRARRAQFRAARTFVDRLAAPVLAIPGNHDLPLF
                     SHGTIKIAQYEITVDLNNASFNENENVKREVASRINDMPEFERGAKVMDFTRMDSRKV
                     PRMLELETPK"
BASE COUNT         2681 a         5336 c         5773 g         2741 t
ORIGIN      
        1 gaaaataagt gaccagcatc agcaggctgg aagccaggca gaccagcgca tgggtgcgga
       61 atccggccgg tcgcccatgg aagctgcgct cgaatccaat caacccgccg gccaccagcg
      121 ccgacagcag gtgcagggcc accgtccgat gatcggggtc gaacaaaaag cgccagtcgg
      181 gcatgggctg tttcctcgat ctgaggtcag gggctggggc gtataaagga cataagggac
      241 ataaaggatg taaaggagac aagagaatta tggcgccatt ctcgaagacc catgggcata
      301 tcccgcttgt gcactcatcg catatcgcgc ctgctcaccc gcggggcggt tttaagccaa
      361 gctgaccgag ttgggcctcg ctgcggcgcc atttgggccg gaccttcacc cacagctgca
      421 gaaagaccgg ctggccgtag agccgctcga tgtccttgcg ggcattttcg ccgatcttct
      481 tcagcatcgc gccccccttg ccgatgatga tcgacttgtg cgcctcgcgc tcggtgatga
      541 tcaggacgcg gatcaacggt ttgccgggac gattttcgtc gaaaatctcg gtctgggtgg
      601 cggtggcgta gggaatctcc tggcccaggt aacgaaagag tttttcacgg accatttcgg
      661 acgccatgaa gcgcaggtcg cggtcgctga cctggtccgg ctcgtagagc agcgggccct
      721 cgggcaaggc gtcggcgatg gcgtcaagca ggagcggcag gccgcgaccg gtccgcgcgg
      781 cgatgccgaa ggcgcgctgg taattgaaat ccggcgcctc ggccgggaag aagttgcccg
      841 ggacgcgatc gatcttgttc cagacgaccc agaccggacg gttggtgcgg cgcagcatcg
      901 ccagcacggg ctcctcctcg ggcggggtca cggcgccgcg cggtgggcgg cgggcgtcgc
      961 gcagatggag gatcaggtcg cagtggcgca gcccgaacgc cgcccattcg accaggtgtt
     1021 cattgaatgc gtcggccggc tccaggatgc cgggcacatc gaccagcacg gcctgcatcc
     1081 ggtcgtcggt gtagatgccg cagacgcgtt cgcgcgtcgt ctggggccgg tccgacacaa
     1141 tggaaacctt ggcgccgacc aggcgattga ccagcgtgct cttgccgacg ttggggacgc
     1201 cggccagccc gacgaagccg acgcggcggg gcgggtgctc gggcggcggc gagggggagt
     1261 gcggcggatc ggggtggttc atggcatgcg ggccgaacgg cggatcaggc cctcaactcc
     1321 ttcgtcgcgc agcaggtcat cctcggccgc cgccatcgag cgccggcgcg cgggcgtatc
     1381 gtgctcgtaa cccagcaggt gaagcaggcc atgggccagc aggcgggcga gatgccggcc
     1441 aggatcgatc ccctcggcgc gggcggccgc gcgcgccacc ggcagcgcga cgacgatatc
     1501 gccgacggcg ccggtcgcgc cgctccggcg cgggtcgccc ggatgcagcg gaaaggagag
     1561 cacgtcggtg ggttgatcgt gcccgcgcca gcggcggttg aggcgccgaa tggtgggtcc
     1621 gtcgcacagg acgaggctca gttcgtgtcg ccggtcgagc ccggcgcgcc gcgccagccg
     1681 gcccagcagc tcggcgccga gcggccccaa gtcttgggca tggcggccca ggcgcccccg
     1741 cagcgaccag acaatgcggg gcgtggccct gggcgtcatg gggcggtgga agtcagcgtt
     1801 ccggtggccg gcgtctccgc cgcctcggtg gagggggaag gcgtctcggc cggcgccggg
     1861 ggctcgcctt cggtcaactc caggctggcc gtggtcagcc ccgcctgggc gagcgtggca
     1921 gtgctcgcca tcgaggcgaa cagggtctgg gacgtgacgc cgtcggcgat gaattgcttg
     1981 agccgttcca tttcgcgctc gatcaccagc gcgtcctcgg gcacggggct ttcggcgagc
     2041 gcctgctcca tcacggccag gacctggtcg aagcggccgc gactgaacag gagggtggcc
     2101 agccggcgct gcgcctcgga acgaaccgaa ctggtcggat acttgtcaat caccgtccgc
     2161 agactgtcga tcgccgcctg aacctgcccg cgcagctcct gcacgcgcgc catgcgcagg
     2221 tagagccggc tcttttcctc ggcgccgagg gcttcgtcga tctgctgatg gtagagcgcc
     2281 tcgctggcat tgaaactggc ttcggcggct tcctcgttgc cggcctggag ctggtagtcg
     2341 ccgatggcga agaggatttc ggggcgctgg ggactgtcgg gatgctgctc caggtattcc
     2401 tcgtagacgg cgaccgattt ctcgggctgg cccatctggc gatagagcgc ggccatccgc
     2461 tccaggggga acatctgctc ctcgggacgc tggtcttcgc gcgagaggag gtactggagc
     2521 agttcgagcg cctgatcgta ctgctcatcg gcggcgtaaa gctgcgagag ctcgaacagg
     2581 aggcccggct gctccatcgg caggctgttg gaggtcgcga tcgcctcggc gatcgcctgc
     2641 tcgaactggc gctcctggcc gtaggagcgg atcttgagga cgcgcgcgcc gaggccctcc
     2701 tcggtctcga ggccgaccgc gtcgacgtag tcgtccagtt gctcgcgggc gcgggtcaga
     2761 tcgccgtcgt tgaggtagca catcgccagg ccaatgcggg cgtcgatcgc ggccttcgtg
     2821 tcgggatgct gctcgatgat ttcctcatag gcgaactgcg cattgagcac gtcgccttcg
     2881 cgcgcccact gattggcctc ctccagcttc tcctcggggc tcttggtgga gcaggccgcg
     2941 gccagcaggg caatcaacag caggccgctc agcgggcgcg gccggtcgtg gcgcggccgt
     3001 cccggcagcc acctgagact gagaatgtgc atgggtgatc tgaattcctc tgacaaaggc
     3061 tgaacccgcc cccccaggcg aggcagcgcc caggaccgaa cggcggattc ccctgtcagg
     3121 aagcacaagc atgaaaacca ggattaagga cgacgcaggg ggcccgggca acggtctggc
     3181 ggcgcaatca gctccatgga ggtcggatgg ctgagccgtc gtgggatttc gtggtcagag
     3241 ccgacggcag cccgggaatt gtagccgctg cccgggggga tgtcaaatcg ccgacgcatg
     3301 gcggaaacca cccaagcaag tctgaattca ccggcggaat ccgttttggg ggtgagccga
     3361 tggcgatggc gatttcgata gcgataccga taccgtgctc gcgggttatt cgcgctccag
     3421 ttcaagcaac agggcgcggt cgcagcgttg aaagccgagt ttctgataaa acggcaacac
     3481 ggggagccag ggactgatgt ggatcatgcc gaggccctgg ctgcggcacc agttgatcag
     3541 attgcgcagg accgggagca ttacgctctc gtccagggcc gccggcagcg cgtccagttg
     3601 cgtgaggatg ccgaccgggg cgtcgcgccg cgtggggtgg ggcaaccggc gcagcatcac
     3661 caggtagagc gccagcagga gctgctgctc gtcgccggcg acccagacgg tccagttgcc
     3721 gccgctgagc gcgcggtcga gaaaggtggc gaaggccgtc tggtaatggt tggcgtcgcc
     3781 gtcgctcagg gcgggctggc ccagttggcg cagatgcagg tcggccagcg cactggaatc
     3841 cttgaacgtg gcgtgtcgaa tcgtcagcac cggtgggtcc cccaggtttt cgcccctcgc
     3901 cgtcggcgag caaaagagga aagagacaca aaggacctac gggacggcaa gcgagcgcat
     3961 agcgcatccc ctctcccgcc cgctcatcgc ccgtgggaga gttcttaata acccgtcaag
     4021 cccgcgttgg caatgccgac gacgagtgac gccaacggcg aggattcacc agttcagccg
     4081 aggctgtcca catagccctg gtaattgcgc cggacctcgg cgagcgaatc gccggcgaat
     4141 ttttccagga aggccccggc gacggcgtag gccaccagcg cctcgcccac cacggcggcg
     4201 gcgggcaggg cgcagacatc actgcgctcc ttgctcgagt cgaagggttc cttcgtcttg
     4261 acgtcgaccg agccgatcgg gcgcatcagc gtcgaaatcg gtttcatcgc cgcccggacg
     4321 acgagcggct cgccgttggt catgccgcct tcgaggcccc ccgcgccgtt ggttcggcga
     4381 aagaagccgc cgctggggcc gtgcccctcg ggccacgcgg cgggatcggg cgaatgttcg
     4441 atcgcatcgt gaacctgact gccgcgccgg cgcgccgcct cgaagcccag cccgatctcg
     4501 actcccttga tcgcctgcat gctcatcatg acctgaccga tccggccgtc gagtttttca
     4561 tcccagttca tgacgttgcc caggccggcg ggcagccccg tgacgatgac ctcgaagatg
     4621 ccgccgacgg tgtcgccctc cttcttgcac tcatcaatca gcgcgcgcat cgcggcttcc
     4681 acggcagggt cggccacgcg cacgtcgctc gcctcggcgc gctcggcgat ctcgcgcagc
     4741 gccagcccgc tcacgtcggc cttgatccgc tccatctcga ctacgtgcga gagcacggcg
     4801 atgtcgaact cggccagcag ggccttgcac agcgcgccga ccgccacttg cgtcgtggtg
     4861 gcccgggccg aggcgcgttc cagcacgttg cgcgcgtcgt cgtggccgaa cttctgcacc
     4921 cccggcagat cggcgtggcc cggccgcggc cgcgtgatcg cgcgcttgcc ctgaacctcg
     4981 ggctgcggat cgggcgacat gatctcggtc cagttgcgcc agtcgcggtt ctccaccacc
     5041 atcgtcaccg gactgcccag ggtgaagcca tggcggacgc cggagacgaa acggacctgg
     5101 tcgctttcga tcgtcatccg gccgccgcga ccgtagccgc cctggcgccg cttcagctcg
     5161 cggtccacct gctcggcgcg aatcggcagg ccggccggga ggcccgagac gatggccgtc
     5221 aactgggggc cgtgagattc gcccgaggtc atgtaacgca gcatcgcgcg gctccttgag
     5281 gggacatggc ggacaggcgg cgcctccggc cgctgcccgt gcgtcgttcc atcgtagcgt
     5341 tcgcgccggt tgctgcccag cgttaaccgt gcgcgaaggg ctggctccgt caccggccac
     5401 gccgttcccg ggccaatcgc ggcggtctgc gccgatcaag cggcccgcag gtcgcggtag
     5461 cacgacgcca gccggcgggc gctgctcgcc accgaaaagg cggcctggac gcgccggcgg
     5521 gccgcttcgc cccgccggga tcgcgccgct tcactttgga tcaattcacc cagcgcttgc
     5581 gccaggtcgt ccggctcggg ttgcaccagc acgaggctgc ccgaatcgcc gtccacttcc
     5641 tcgatgcccg gcagccggct ggcgatcacg ggccgggcgc aggccatcgc ctcgatcagc
     5701 ccgaggggca gtccctcgtg ggcgctgggc aacatgaaga tgtccatcgc gcccagcagc
     5761 ggagcgacgt cgcgctggag gccggtaaag atcacgcgct cgcccagccc gagggcatgc
     5821 gcctcggcgc gcaggccggc cgcgcacggc ccctcgccga ccagcaggac gcgcaaccgc
     5881 ggttccgccg ccgccagccg ggcggccgcc tggaggatca ggtgctggtt cttgaccggc
     5941 gcgagccggc cgaccgttcc caccacgatg gcctcgtcgg caatccccca atggcggcgg
     6001 accgcggcgc gctcgacggc cgtcggcggc cggaaccggt ccgcgtcgac cccgttgacg
     6061 acgacctcga cgcgatccgc gcgcggcaga cgcgccgccg ccgcgcggct gcacgccacg
     6121 accagatcgg cgttccggaa gcaacgccgc tccagggggc gctggtagcc gcgcgcgcgg
     6181 ccgggcgtgg catgcacgtg cgtcaccagc ctctcgatgc ccagcccgtt gcggaaattt
     6241 tccgtcagga acaccgacgg gatgaggtgg gcatgcacga cgtcggggcg gagccgccgg
     6301 atgtgcgcgc ggaggcgtgg aaccagtcgg gcgtcgaaac ggctgcgcgc cagctcgcgc
     6361 ggagcgaagc ccgcctcggc aaaggcggag ctcaggctct gcggccccca cagcgccgcg
     6421 accgcgcagt caacgtccag cctgcccagc tcgcgcagca ggctgagcag gtaggtctgg
     6481 gcgcccccca gccgcaacga atcgatgaca tggagcactc gcatgcggaa caccgttgtc
     6541 ctcgcatcgt ggcaatgacg cggcgggctg tcaatccgcg cgaggacgag cgacgggaga
     6601 ggcgtgacgg cgcgaggatt tcctgggagt cgtctgcaac ccgccgcggg tttgccggct
     6661 cttgtgaatc ggcggcatga gatccgggcg tggcgcctgg tgagagtcta acggcaacgg
     6721 ccgcgcgcag ggttggcgta tggccgcgcg gaagggaatg ggatatgcgc tggaatcggc
     6781 gacaagccgt gatccgctgg acatcgccgg gcctcgtccg gctgttcagc attggaatcc
     6841 tgtgtgcggc gttcctcgcc tgcgatcggg gtcgccccgt cgaaggcttt tatctctcaa
     6901 cgattggctt cgaaccgctg gatccgatcg aaatcgccac gaccaaccag ggcaacctct
     6961 cgatgggctc catccgctcg cccgctactt cctcgttcgg ctcgcccaat tccaagatcg
     7021 aaattcgggc cggcagccgg ggatttgtcg tccgtgaacc gcccgacaag aagggatttg
     7081 cgcggctgtg ggaggtgatc gtcgccaggg cgaaaggcga gacgcttccc gaccagttcc
     7141 cgctgttgca actgaaggcc tcggcggagc gtcccggcga ctgggatctg gtcggtttgc
     7201 gtgtggcggg aaatttccag gtgttgccaa acccggttcc ttcggccttg catcgcgtcg
     7261 aggacccgca ggtgcgggag tatttccatc ttcaggacga gttgggccgc gatggcgagg
     7321 ccctggcgct ggcggagaaa ctgctcgcgg cccaccccaa cgatccctgg attcatgcgc
     7381 tccagctcga tgcgctgctg gccgccaaac ggatcgatga ggcggaggcg ttcgtggcgg
     7441 cctcgcgcga cgagctgctg aagcacacgg agtccgggtt cgcgcatctg gtgcgggagg
     7501 cggaacgccg cgtagtcggt ctgcgggccg cagccgaagg gcgcaacgcc gccgcgctgc
     7561 cgggcgtcgt cacgttcacc atgcagtggc ccgacgacct cctgccctat gaggcggtga
     7621 tggacgggct ggagcgggcg cttgaatgtg atgtcatggt gattcagggg cgggggctga
     7681 ggtcggatcc gcgtggcccg gtccccaact tcctgcactt ccaggtgatg gcgaagattg
     7741 cgcgcgcgga ggcggccggg cgcctgctgc tcggccgccg cgacgacgcc ctgcggctcc
     7801 aggccggggc ctgttggctg gggcagatga tgcagcggga tgggacgctg atccagtgcc
     7861 tggtcgggac ggcagtgcgc cggatcggcc tggagggcct ccagacctac gcattcaacg
     7921 cctgcgagac cgatggcgac tttcgtgagc tgtggcggca tctggagcgc tttgccgcgg
     7981 cgcccgtccc gcacgcagaa gccgagctgt ttgccggcga aggctggctg ggcctgaaac
     8041 tggatgagct gaacgccgga cccacgcctg gcgaggcggc aaagcgtgtc cttgtgacga
     8101 aagcgcagct tgaattgctc cgcatggcga cggccgcacg ccaccggctg gtgacccagg
     8161 gcgatttccc caatccgctc agccgcccct tttcgccttt actggagcag atccccgacg
     8221 accctttcac caggcccgag accgtcgcca atgacgcgaa aacatcgaca agtgctccgt
     8281 tgcgccatgc tgttcgtccg ggcgacgaga ccttcgtctg ctggagtgtc ggccccgacg
     8341 gactggatga tgccgccggg atcccgtacg acccgaccaa cggcacactc agcgcggggg
     8401 atctgtttgt cgtcgtgccg cgcgagcgcg agatcccctt cccgcgcgag ggggttcgcg
     8461 tcgattccgt tcaggaattc atggagcttt ttcccaacgg gctgccggtc gatccgttcg
     8521 ccgacacgaa aacccgccgg ctgagcctga tcgaccggcg ccccgtgacc gtgttcagcc
     8581 atggaccgga caccgacgag cgggtcggcc cgccggatcc gcggatctgg acctgggaac
     8641 ccgagagccg cgagtggctg cccgagccgc agtggacggc gatccaggag gcccgccggg
     8701 gatttcaacc ggcggctgcg gggatgctgc cgggaagccc cggcggggcc gcgccaggat
     8761 ttcaacccgg ggccggggcg ccgattcaac ccggggcccg accgccggcc atgccagccg
     8821 atggcgcgac gccggccgcg gcgccgctgt cccacccgga agccccctac gatccctcca
     8881 acggcaccat cagcgacggg gatctctact gggagatgat ggggcgcgag ggagccggtg
     8941 ggagatgagg ggacgtcagt cagccggtcc gcggtaagta gttcgttcga agatgcatcc
     9001 ctcgttctga tcgattcgag cggttccggc gtccctgcgg gactggccgg ctggtcgacc
     9061 cggcagggta gcggccgttc gaatcggttg ttcaaggctc ccccgttttt tccacatggc
     9121 agaccgccgc tgcgtgacca acacctgagc ccccaagggc cctctcagga actcggctcc
     9181 cggctcccgt ccctcgtctt cggctctcga atcgcgtcga tttctccccg gatgttcccg
     9241 ccggtccttc ggccgctgga ccgccagccg acggcgaggg gcggggttgg gagcggtaat
     9301 tacgaaccgc agcctccgca aaaccttgat gtcaaacccc ttgcacatcg cccgccgggt
     9361 cgtgcttaat agagggcctg agtcgacgag ggagcgcgcg aagccctgca cccgagccaa
     9421 cgcaacggag tcgaccctcg ccccggcgag gggaggtccc gttggcggcc gtgccagact
     9481 ttgccggaga tcgtcgcacg gtggtccctt cggtgcgcaa gggtccccgc tcagcaaccc
     9541 cggaatcgag atgttggagt ttctgacgca tggcgcgccg ccgaagcgaa gctgctgccc
     9601 ccgctgccgc cccgtttggc cgggccgcgt tgcccctggc ggcggcgttg cgtctgggca
     9661 gccagtttcc actgcggctg gaactggtgc tgaccgagtc ggccgcggcg ggcaagctcg
     9721 ggcccgatga agatccgttc gtcgtgctga gcgcgcacgg cgggcgttcg cgcgtctatc
     9781 tgggtcgggt tctggcgggc gaagccaatc tgctcagtca cgtggcggtg aaactggcgc
     9841 cgcggcgcgg caccccctcc ggtctggaag gccagagcct gacggaggga caacggctgg
     9901 agcgctggga gaaggaatgg cagcactacg ccgcgctggc cgagtcggag gaagcgattt
     9961 cgccgatcct gttcgttacg ccgggcgaag cgctggccgg cagcgccgcg cggccgatca
    10021 cctggccgcc gctggtctac tgcccgatca cgcggacgct gctgcagccg cgttcccgcg
    10081 aggggggccg gctgacgctc tgccgcgacg acgcgacgct ggtggagcac ggcctgccgc
    10141 cgcacggcga acgctatgcg ttcctgtggg acgccgaggc ggcgctgcag ggccaggaac
    10201 cgcgattcta ccaggtgccg gtgccgggcg agccggtgct gggcccggcg cagccgtttt
    10261 cgcagtgcca gcgcgagatg gccgagctgc tggccgatcc ggcgggcctg attgatctga
    10321 cgacgccgga cgggttggcg cgtctggccg acctgaccca ggcgtttccg ggcctgacca
    10381 atccggagat ccgcgaagaa ctgctgcggc gcgatccgac cgagttgttc atcccatgga
    10441 acctggatga agcgccggca ctggtgatgc agctcaatac gtttcactgc gacgagttca
    10501 gcgacctgct ggggggcatt gaggagaagg agttcgtcgc gcgctatcag gggccggtcg
    10561 cggcggcggg ccagcggcta cggctggcgc gggcgccggt cggcgagcga ttcaaccgtc
    10621 ggctgttgtt ctcgggcgac ggctcggggc tggatgcgct ggagatcttc cggctcaaat
    10681 ggtcgctcgt ggggcagatc gtccggtcaa ccctggagtt tcagcggcgg acgggctcgc
    10741 cgcacctgcg cctcgccccg gaacacgtga tgatccacat cccggagagc ggcggctacc
    10801 tgccggggtg ctggacgttt cagacgcggc tgatcagcct gggcgctccg accctggaga
    10861 ttccgggaca gacggatctg ccgccgatcc acgttccgcc ggtcggttcg gacccgctgt
    10921 accaggccga gatcatccgc aacagctcgt tcggtctgac gcagcgcggc gagttccaac
    10981 tgaccgggct ggaaccgagc ggcgagcgca ccgtgatcgt cgcccagctg catcacgagg
    11041 gtctgggcct gcgctggctt agcccgaagg atcacgttca tgtctcgctg ggccgccatc
    11101 tggtgggcga agcagcgatc gactttctga gccgccgcga cgagaacaag gagtattccg
    11161 ggcggacgct gtatctacgc agtctgccgc tggacctgga caagacgatg caggccgaat
    11221 tgcagaagct gcgcggcatc cgggtcccca acgcgcagtt ccggctgctg ccgacgctgc
    11281 acgttccctg cgacctgtac agcctgggga tgctgctcta tcgcgtcctg ctggtgaacg
    11341 accatcaggg gatcggcgac gtggcgctgg cgctggatga gttgcgccag gacctggcct
    11401 cgctggccga cgtggaggtt gacgcgggca aggaggcgtt cttctgggat gcgctgctca
    11461 gcgggcatcg gcggcccgaa acggccgagg tcttcgaccg gcggcagatc ttctatcatg
    11521 agcatcagcg gtcggacgac cggcccaatg ccatcccccg gcggctgtgg cacgaagcga
    11581 tggtgatcgg gctgcaactg gtcacgcagt tcgaagggtt cagcttcgcg gcgcatcacg
    11641 gcgatttcga cgagagctat ccctccgagc gactggaacc ggtcgtccgg cgggtcgagg
    11701 aattggggcg ccggctggac gcggcgttgt tctcgttcac ggcgcgcaat gccgaggtgc
    11761 gccaggcgct cgaactggtg acccaggaaa tccgagtcgg ttgacgaaat cagcgcggga
    11821 tatctgacgg acgatccatg tgtgcagcgg cttcgactcc ctttcttcgc ctcgaggtga
    11881 tcgcgggccc gctcgacggg ttgagcttcg aggggcgcaa ggcatcggtt cggatcgggc
    11941 gctcggacgg aaccggcccg ggcgacgtgc gcaacgacct gatcctgcag cgcgactcct
    12001 acgcgtcgag cttccacgcc gaaatcacgc atcaggaggg gagctggcag gtccgcgacc
    12061 tgaacagcac caacggcacg tgggtcggcg cgcgcaaact ggagcggggc gagcaggttg
    12121 ccgtgggcaa cggcgaagtg ttcgtgatcg gcaacacggc gatccagttg accgagaagc
    12181 cggcgggcct gccggcgatc gacgcgtcga tctcggcgat tctctcaccc gccagccaga
    12241 ccatgatcaa ccgcgcgcgg acgctggcgc gcgagctgaa ccagccgtgg gtgggcgttg
    12301 cggcggtctt tcgcgtgctg gcgcaatcgg atgcgccgcc gctgcccgat tttttcaagg
    12361 cgctgggcgt ggatcccgcc gccgcggaaa aggcggtgct ggatttcgag tattggaagg
    12421 atgagctgga ttggatcggc aaggaggtga tgcgcggccg gcggccggcc gggcagatcc
    12481 gcaacgtggt gccgacgccg cggctgcaac tggtgctgac gctggccgat cgggtgcgcg
    12541 agaaccagaa cgagccaaac gtcgaaccgc agcacgtgct ggcggcgatg atggtcgagc
    12601 cgggcaatat cgtgctgcgc gcgctggcgg aggcgggcca tgcgacggcg gcgctgcgcc
    12661 aggcgaccga gaatttcatg aaaccgccgc cggaaaagaa agccgagccc gaaccgctcg
    12721 ccgagccgga gcccgaaccg gccgagacga ccgcgcggat ttcgctgccc ggattcaagg
    12781 ggactcccct gcgcaagccg gtcgatcctg aaacctggct gctggcgcgc caggcgatgg
    12841 accgcatcga ggtggtgcag aacgactaca gcatggccga tccggccgtg cggttcgatg
    12901 ccttgtgcac ggtgatccgc gatgcggtgc agaaggcgcc ggggcccaag cgcgcgctgc
    12961 tgatcgaaca gttgcattac ctgtttccgg tgttggaaga ggcggtgctg ccgccccccc
    13021 cctcgctgtt ccacgagcag acgccggcgt cgctccaggg gggcgaaatc accgatcatg
    13081 agctggaggg gacgggcgac cgcgcgcgca tgcgcccgtc ggacgacgtc agcgccacgc
    13141 tcaaggccga tcggtcgcag ccgcaacgcg aaaccacgct cgacacggac tacatgctgc
    13201 aacaggtgtt cgcgacggag aagctcgcgc cgctcggcgc cagctcggag agcgacagcc
    13261 agttcctgcg cctgatgcag ttgctctaca cgtttgcgct ggacatggag cgcctggcca
    13321 aggggatggt gcagtcgctg caaggggggg gcggcggcga gtcgcgctac ttccttccct
    13381 tcacgatgca ggatctgaaa atgatggtgc agaagatcct ggagcagaag gccgatccgg
    13441 aaaccgttgt cgacatccac cagtatctga gcgacctgag ccactggctg atcgcgctga
    13501 tcgccgcgca tcagaagggg acggtgcagt ggtatggcga gttgtgggag cggatcagtc
    13561 cggcgggcgt gcgcaacgag acgaaggttt cctcgctgaa caaggtgctg ggccagagca
    13621 aggcggatct gtgggaaacg tatgagcggc tggcgcgcga cctcaaaccg gagatcgttg
    13681 aggaccagtt caaggaaaag ctcagccagc tcgtgacgga agaattccgg cggctcagct
    13741 cgcgccggga gtagcggcgc gcggcctcaa ggggcctgct gcagcgcctt gtgaaccgac
    13801 gacagcaggt cgatgcgggt gatcggtttt tccaggaagt gggcgcccgg cgggagcgac
    13861 cggttgagca gcgctgtcgg ttcgctatag cccgacatga aaattgtccg gatgtcgggc
    13921 ttgatcgcgg ccaggcgccg ggcgacctcc ggaccgctca tcttcggcat gaccagatcg
    13981 accagcagca gggcgatctt gtcgcgctgg cgttcgaaga ggcgaatcgc ctcgtcgccg
    14041 ctccccgcct cgtggatcgt gaacttctca ccgcgcagca tctgcgtcaa cgtggcgcgc
    14101 accttgtcgt catcctcgac gagcagaatc tcctgaccga acccgtggcg gatcggccgc
    14161 ggctccgggt cgtcggcgat cgactcgggc tggtcggcgc gcggcagata gatcttgacc
    14221 gttgttccct tgcccggttc gctgtagagc cagatgttgc cgccgctctg tttgatgatt
    14281 ccgtagacgg tcgacaggcc cagcccggtc cccttggatc ggtccttggt ggtgaagaaa
    14341 ggttcgaagg cgtggcgcag cgtttcctcc tccatgccga tgccggtgtc gctgatcgac
    14401 agcaggacat gcgggccgga cttgacggag aagatcgccc cgccggcgaa atcatccgag
    14461 aggaagatgt tggccgtctc gatcgtcagc gtgccgccgt tgggcatggc gtcgcgcgcg
    14521 ttcaccacca ggttcatgat gatctgctcg gcctgggtcg ggtcgaagac gacgttgcgc
    14581 agatgcggat caaggcgggt gaggatttcg atatcctcgc cgatgagccg gcgcagcatc
    14641 ttgtccagat tgcggacgat ggcgttgagg ttgagcacgc gcgggcggag catcttcttg
    14701 cggctgaagg ccaggagctg ctgggtcagc gcggaggctt tctcgccggc gcgacggatt
    14761 tcctcgatat cgggccggat cggggaatct tcgggcagac cgagcagcgt catttcggcc
    14821 tggccgagga tcacggtgag caggttgttg aaatcgtggg cgacgccgcc ggcgagccgg
    14881 ccgaccgcct ccagcttctg agcctggcgg aatcgttcct gcaagtcgag cagctcctcg
    14941 tccttctggc ggacgatttg ggccacatcg aaatcgtagg acatgccgcg cgccgagccc
    15001 gacaggtcgg tcaggtgaac gaagagcttg cggctgtcgg ggtcgcgcat gccgctgagg
    15061 cccatatagc cgtagctgcc gtcgtggcgg cggatgcgca gcatctggcg aaaggcgtaa
    15121 tccgaagtgg tcttgaaggg ggtatggagc gcgcgatcct caggatggat gaactcgagg
    15181 agggaatggc gcgccgaatc ctggacgcgg atgcccagga gggcggccag cgccgcggaa
    15241 gcggagagga tctggccatg ggcgtcaaga aggcagcact tgggagcctg cggatcaggc
    15301 ttgggagcat cggcccgggt cataggggat ctttttcgga cggaatgagc gtgcaggata
    15361 ccttatgtta taagtaacaa tcgacgataa agtaaacgcg accgtgcgaa attgttgatt
    15421 tccaggtcga cactatccca ccataggacg cgcgggtctt atccgcgccg ccggggtagg
    15481 atctcctccg tgatgcaact cctcatggct tcccctgtcc ccactccctc cagccgcgct
    15541 ttcaccctga tcgaactgct gatcgtcgtc gcgatcatcg caattctggc ggcgatcgcc
    15601 gttcccaatt tcctcgaggc ccaggtgcgg gccaggattt cgcagacgca tgccggcttg
    15661 cggaccaccg ccgtcgcgct ggaggcctgg cgggtcgatt acaacagtta tcccagccgc
    15721 tcgccttccc aattccagat cctcgaggcg ctgatcccgc tgtcgacgcc ggtgccctac
    15781 gttgccgaga ttccctccga tccgttcgtg gccaacctgc cggccagcta tcccgacggc
    15841 atcaactgga acctggctta caagaatcat ctgggcgagt ggacgtttcc ccggccgttt
    15901 gactaccagc cgcgccttcc gaatgccctg tgggcatccg tcacctcgca tccggagtcg
    15961 gcgcgctggg cgctgcgcag cgccggcccc gacattgtgc cgacctggct gggttacggc
    16021 gcgccggcct acgatccgac caacggcacg gtcagcgccg gcgacatcta cttcaccggg
    16081 cccggcatcg gtttggatca acctgcggtt cccctccttt gaagaagatg cgcggtacga
    16141 cctcggtgag gccgtaccgc gtctgcatgg gccgctctcg aatcaacccg ttcatttggg
    16201 tgtttcgagc tccagcatcc gtggcacctt gcggctgtcc atgcgggtga agtccatcac
    16261 tttggcgccg cgttcaaact ccggcatgtc gttgatgcgg cttgccacct cgcgcttcac
    16321 attctcgttc tcattgaacg acgcattgtt caggtcgacg gtgatctcgt actgggcgat
    16381 tttgatcgtg ccgtgcgaga acagcggcag gtcgtggttg cccgggatcg ccagcaccgg
    16441 cgcagccaga cgatcgacaa aggtgcgcgc ggcacggaac tgcgcgcgtc tggcgcgctg
    16501 cgtgatgtcg cccgagagca ccaccacgat g
//
LOCUS       DAOPCO010000056        16407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3594_length_16407_cov_4.724560, whole genome shotgun sequence.
ACCESSION   DAOPCO010000056 DAOPCO010000000
VERSION     DAOPCO010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_16407_cov_4.724560"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLS90_06105"
     CDS             complement(<1..784)
                     /locus_tag="PLS90_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012929488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPK02012.1"
                     /translation="MKLDSKVPGGPIQEKWDRHRFEMKLVNPANKRKYKILVVGTGLA
                     GASAAATLGGLGYDVHAFFIHDSPRRAHSIAAQGGINAAKNYPNDGDSIWRLFYDTIK
                     GGDYRAREANVYRLAQVANNIIDQCVAQGVPFAREYGGYLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYSAMMQQVNAGNVKLYPRREMLDLVIKNGQARGIICRNLVSGEIESHA
                     GHAVALCTGGYGNLYYLSTNAMNSNATAAWRCYKRGAAFANPC"
     gene            complement(798..1475)
                     /locus_tag="PLS90_06110"
     CDS             complement(798..1475)
                     /locus_tag="PLS90_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPK02013.1"
                     /translation="MPVSCCDLWTTVGKKYLAAAAGLVFLVFVIVHLGGNLLLLLDNP
                     NLYNSYSHHLVSLGPLLWFGEAVLLAFFLLHAVIGTLVYIRAREARPVGYHEIADAGK
                     PSRKTLSSRTMIWSGIVILVFTILHLITFKFGEHYTVVVDGIEMRDFHTLVVEVFQKP
                     GYVVWYLLAMVLLGLHLRHGFWSAFQSLGVNHPRYTPFIYGLGTGLAVLLAFGYIVIP
                     IYVYFKY"
     gene            1876..2475
                     /locus_tag="PLS90_06115"
     CDS             1876..2475
                     /locus_tag="PLS90_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK02014.1"
                     /translation="MATETVDTHLDPARWLDEHGDLLYRYALARVRDAEVAEDLVQET
                     FVAALRGQRNFAGRSSERTWLVGILKNKIVDHLRRKWREQPLSELEPAEQAEDFLDLL
                     FTRGGRWKEGAGRWGDDPAAGVRAADFQRVLAACLEGLPGRLADVFILREIEQLPSDE
                     VRQVLNISPTNLWAMLYRARMRLRGCLEANWLGHAEGKR"
     gene            2479..2730
                     /locus_tag="PLS90_06120"
     CDS             2479..2730
                     /locus_tag="PLS90_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPK02015.1"
                     /translation="MLTCRDVTRLVSESLDRELPPLLRFRLRLHLVLCLFCRRFERQM
                     LALRALAREVAAGEADGPGLPEAAKERIRGRLRGEQAQP"
     gene            complement(2963..5743)
                     /locus_tag="PLS90_06125"
     CDS             complement(2963..5743)
                     /locus_tag="PLS90_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPK02016.1"
                     /translation="MVRTKTARATKPGRNAPAPSRGGKIPLHWVISTHWDREWYEPFQ
                     GFRYRLVYLLDEVLDRLEHDPRFTHFQMDGQSIPIEDYLEIRPEREEQIRRFAQAGRL
                     AIGPWYTLPDEFIVAGESIVRNLAEGLLVARRFGEPSRAGFICDIFGHCSQLPQIFRG
                     FRIATAFLWRGINEADVGRNFRWRAPDGSEVLAFRFGANNGYCDFYFRVRRRAHPADR
                     RRQSLTDAAVEYLATQTGRPPAGLPVLFFDGCDHMELEPLTGELIEDFARRPEAARFD
                     VRLSTLDAYAADLMRSADRITKTIDGELREYGKPPTSVEQQWLIPGVLSSRIHLKQAN
                     ARCESLLTQWAEPFGLFTTASGMPDPAGFLRAAWRLLLQNHAHDSICGCSPDQVHKDM
                     LYRFDQVELIADRIRQGALRHLARHVHRGLKPAPGAAHPPATLLLANPTARPIDEPVD
                     FDVVLPEDTPCFMEWFGFEKKPAFTLHDERGREVAYQRVGQSLHQPEIVWTRGLPPKS
                     PRRFHVHCTARLRIPPFGYTSLAVRPVEGFTRYGTTGLATSPTSAENEFLALGFTLAG
                     QLIVTDKRTEETYEPLLTLENGADIGDGWYHGVPLNDQVHVSSAAPATVALVADGPLK
                     AIFRVELEWTLPARFDFVEMRRSDERRTLRVRHDVTLRAGCDRIEVETTVENNLRDHR
                     LRVLFPSGASAQTAWSDTPFDAVERPIALNPERIQWRELEVETRPQQSWTAVSDGRRG
                     LAIVSHGLPEAAVRDLPERPIALTLLRAFRRTVMTRGEEGGQIQGTHRFRYWIVPLTG
                     AIDPVALTRLGQRLAAGIGQAHHLPDMPPTDPERVDDDGAPLPRTHAYLRVEGEVIVS
                     AVKAADDGRGTILRLFNPGRSAANCVVHRAGLKQAWRCDLEETPAEPLRVSRGAVELN
                     LPSRRIDTLRLL"
     gene            complement(5816..6484)
                     /locus_tag="PLS90_06130"
     CDS             complement(5816..6484)
                     /locus_tag="PLS90_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPK02017.1"
                     /translation="MNSPQPLALDLIVYDLDGTLVDAFDDIARGINAVFSRHQLPQLP
                     MAQVRGHVGDGARMLVRRCLADVGALDRFDDIYRGYREYYGRNPAGSARLYPGVLETL
                     DAVRARGIRQAVLTNKPAEVVAGVCDRLGIAARVDGLWGELEGVPRKPAPESLLRVIR
                     HFGFPPDRCAMVGDALPDHRVARAAGVRMIAVAWGLLSRRQALALRPDRVIDRMPELL
                     DLLA"
     gene            6749..7435
                     /locus_tag="PLS90_06135"
     CDS             6749..7435
                     /locus_tag="PLS90_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HPK02018.1"
                     /translation="MTVNQPEPIPEKGFAIKDCALTAIATGRRAQNLRELRHILQEIH
                     PGSIYYHFWGGLLRPRYYDREYKNDFANWAATSLHHQTLAERLSVIDPNDYADIERLR
                     EELLDTIDDVLDATEQVPWAVADEQFHFMRSQIVVFSTHHLVAEPEQLKEVVPAMTIS
                     SVFYHFIDARSRTDAHVDDFRAWLGGMSPRYDDLCERLAQVDPYFESLPRLREQLSEL
                     LNDYFGGGAS"
     gene            7432..8673
                     /locus_tag="PLS90_06140"
     CDS             7432..8673
                     /locus_tag="PLS90_06140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02019.1"
                     /translation="MRSMLEAYAEVAGADVIDQLHQLAEPLRGKRVVHVNSTRIGGGV
                     AEILNKLVPALEELGIESIWEVLTGESDFYQCTKGFHNAIQGTAADIPPALLRVYEET
                     NAENAERLRPVLAEADIVLIHDPQPAALLRHIPERRGKWIWRCHIDASRPYRPVWRYL
                     RKFVSEYDASIFSLAAFAQPLPHNVYIIPPSIDPLTEKNMDLPEEEIAAVRERFGIDP
                     DRPMILQVSRFDRFKDPLGVIAAYREARKFVPNLQLVLAGGGATDDPEGDVVLTEVQQ
                     AAAGDANIKVLLLPADAHRTINALQRAADIVLQKSLKEGFGLTVTEAMWKGKPVIGGN
                     TGGIRLQLVNHETGFLVNTPEGAAMRIRYLLFHQKQRLKMGRNAREFVRENFLVTRHM
                     REYLTLILTLIHGGEERIVLG"
     gene            8742..9947
                     /locus_tag="PLS90_06145"
     CDS             8742..9947
                     /locus_tag="PLS90_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK02020.1"
                     /translation="MRFVRPLVAQAALLWVAACGGGGDGAGQPEITFAASLLPAEQAK
                     YNTILDEFTRQTGIPVKLVAVQYAQIRSSVEAEAQAGRGKLDLVELDLPQLPVMRPLM
                     QPLDGITSGIEALRDAVAEGAWEVTTAPGAGGAERTLYLPHRLNWQAMVYDSEKLPEP
                     PGTWEELLAAARAHAGAIGLKCARYEGLICDVFPFIWQAGGDPLRPDSAEVVQAIEFL
                     RELSAYFNPAVRSYQETPVLEAQRLGEIVLHFNWPFVVPLLREQKLLPSPMKVAPLPA
                     GPAGRATVLGGGYLGIPAAAPHREQAARLMEFLTSVEGQRSLVRELGWFPMREEGWAA
                     MSAADRELYAGYLAMRREVRARPAVADYDALSRRWQDAIYEILFEGADVKGRLKALQS
                     EIEEPGAEG"
     gene            10051..11352
                     /locus_tag="PLS90_06150"
     CDS             10051..11352
                     /locus_tag="PLS90_06150"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPK02021.1"
                     /translation="MMIRSLVLACLIGVISPGHPGFAQPEQPLRPGEYQPPAAADLMA
                     SYQRALAEAHSYSLDVRQRVVLPNLDVATTMVIAVERPNRLLYVAEDQPPGMFAGRVA
                     ADGEKLYLHRPQQNLVVEREAPADLAELAGDPEIQILLANNLELGLDLVLPMLAEEPT
                     SGPTPYEYVGHETWSGRLLHHIRNRRQIDEGLSLRFDYYLTDGDPAQLVRIQPDFGEL
                     MRVMMRNHGEDAAALEAASAQAEMYFDIGTWQLNPELEGELFAFAPPATAEVSDTLFA
                     QEKHPLVGSEAPDFDLPLLDGGQVQLSSHEGSRIVILDFWATWCGPCRVAMPIIDKVS
                     EEFAPQGVQLYAVNLRETPETIKGFLSGQEFAPTVLLDAEGTVSDRFGVQGIPTTFII
                     GKEGRVQAAYVGALPNLEQRLRRDLKKLTGGESLLPAGADE"
     gene            11449..12303
                     /locus_tag="PLS90_06155"
     CDS             11449..12303
                     /locus_tag="PLS90_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK02022.1"
                     /translation="MKHWAQQRMIYGLAAVPALLFLLFQIWPLGHAIQTAFTNETGQW
                     TLEPARRVWGDPLFRRSLGYSFLIPVVSVALEAGAGVAMALWFYFGLGRARGFWRTIA
                     LIPFAVPEIVYLLTMKLVFRQNGYLNSLLWHVGGSEATVGWLEPGTPLMVGVVILVDA
                     WRVTPIVLLIVLAALEQVPESYLEAARIDGASVGQIAWRILMPLILPALLVALALRAV
                     DAFRIFATPLVLVGIDALPVLTSVAYHYQEFARDGAAANVAALTLAFTLLLLTGLTLL
                     GMKRRRSS"
     gene            12333..13157
                     /locus_tag="PLS90_06160"
     CDS             12333..13157
                     /locus_tag="PLS90_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK02023.1"
                     /translation="MAPSRGARRGLTWAGLALLLLVNCIPPLLVVRQAFAPLGEPPGM
                     PLRLLPGRLSLGHLAQLLETQNLLGRIGLSLWVAVATTALALALGFPAGWGAVHLRRL
                     GDVMTRLSLASRILPPIAVAIPLTALLIPVGLYDHPFGLGLVVAHLTLGLPFAILLAY
                     AAFREVPGELEESARVDGCTPLGAFWRIALPSVRGSLAGAMILVFLLSWDEFAYALLI
                     QLTNRTLPPLIYYFTSYGDMGSASTLAVVMLVPAAGVIFVLQGLLTRGALTGGVKE"
     gene            13310..14164
                     /gene="otsB"
                     /locus_tag="PLS90_06165"
     CDS             13310..14164
                     /gene="otsB"
                     /locus_tag="PLS90_06165"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPK02024.1"
                     /translation="MRTKTTIAPEQSADRIDDFFRRMAEQSRMGTDPRMEGRPRCVLF
                     LDYDGTLAPFRVARLEATPFPGVRRRLGRLLAGRSTRVVIVSGRTVDDLRYLLRLDPL
                     PELWGAHGWEHQPAGGEARVLPLPEAARNGLREAEAWARAGGLGERLERKPGCLALHW
                     RGVSPDVRDAMHDSVRAGWGALAAAGGLELTEFDGGIELRVPGRDKGDAVRAVLETIE
                     WREGVLAAFLGDDRTDEDAFAALKQSLGERALNVLVRDQWRESCADAWLRPPACLRRF
                     LDRWIALE"
     gene            14266..15786
                     /locus_tag="PLS90_06170"
     CDS             14266..15786
                     /locus_tag="PLS90_06170"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013924701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPK02025.1"
                     /translation="MDETRRGLIVVSNRLPYALSRDEAGRLTLQPGAGGLVTALVPVL
                     RNRGGTWIGWSGIESDDRQLQAEAARVLAGATRDAGYQLQPVPLSHAEVELYYHGYSN
                     ETIWPLFHDLQSQCRFDPEYGRAYDAVNRRFAETIARHARVGDFIWVHDYQLMLVGHH
                     LRELGVTAPVVYFHHIPFPSIDIFLKLPRRSYVIRALLGYDQLGFQTARDKRNFLQCV
                     RTLVDGARVRSQGSLHLIRWEGREIRVGAFPIGIDFEEFAGQAAQEEVSRRAWNIHAG
                     LPERQIILGVDRLDYTKGIPHRLEAVRTALKRHPELHRRLTFVQVVVPSRVDVPKYAR
                     LKEEIERLVGEINGEFTQGGWVPIHYVYRALPREELLAYYRTSEIALITPLQDGMNLV
                     AKEYCACTLEDNSVLILSEFAGSAAQLHRGALLVNPYDVEEVADAIHRAYMMPSEERR
                     ARMRRMRRVIRQQHIFWWVDTFMQSAIAKDLSDFPVLEEYDFIPPERVHPEEQSSG"
     gene            complement(15791..>16407)
                     /locus_tag="PLS90_06175"
     CDS             complement(15791..>16407)
                     /locus_tag="PLS90_06175"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPK02026.1"
                     /translation="GGVRFEVRVSALDESPYHHPSAPRASALKTAMAKASAVAPDLPR
                     DTIVIGADTLVVLEDGAYTKPANRDEARRMLARLSGRRHEVITGLALHRVGGETLVDA
                     DVAGVHFRQLDPAAIDRYLASGEADDKAGAYGIQGEGADFITEVEGDLTCVIGLPLLR
                     LEALFLQMTGLALFNGRRPLGIARMAFPDLDRLPPACFQGLRQA"
BASE COUNT         2619 a         4935 c         6146 g         2707 t
ORIGIN      
        1 aacagggatt ggcgaacgcc gcgccgcgct tgtagcaacg ccaggccgcc gtcgcattgg
       61 agttcatcgc gttggtcgaa aggtagtaga ggttgccgta gcctcccgtg cagagcgcga
      121 cggcgtgccc cgcgtggctc tcgatctcgc cgctgaccag attgcggcag atgatgccgc
      181 gcgcctggcc gttcttgatg accaggtcca gcatttcgcg ccgggggtag agcttcacgt
      241 tgccggcgtt gacctgttgc atcatggcgc tgtaggcgcc cagcaggagc tgctggccgg
      301 tctgcccgcg cgcatagaag gtgcgcgaga cctgggcgcc gccgaacgaa cgattgtcga
      361 ggtagccgcc atattcacgg gcgaagggga cgccctgggc gacgcactgg tcgatgatgt
      421 tgttggcgac ctgggccagg cggtagacgt tggcctcgcg cgcgcggtag tcgcccccct
      481 tgatcgtgtc gtagaacagg cgccagatgc tgtcgccgtc gttggggtag ttcttggccg
      541 cgttgatgcc gccctgggcg gcaatcgaat gggcccgacg gggggagtca tgaatgaaga
      601 aggcgtgcac gtcgtagccc aggccgccga gggtcgccgc ggccgaggcg ccggccaggc
      661 ctgttccgac caccaggatc ttgtacttcc gcttgttggc cgggttgacc agcttcattt
      721 cgaagcggtg gcgatcccac ttctcctgga tcggtccgcc gggcactttc gaatccaact
      781 tcatggaagc cgctccgtta gtatttgaag taaacgtaga tcggaatgac gatgtagccg
      841 aaggccagga ggacggcgag gccggtgccc aggccgtaga tgaacggcgt gtagcgcggg
      901 tgattgacgc cgagcgactg gaaggcgctc cagaacccgt ggcgcaggtg caagccgagc
      961 agcaccatcg ccagcagata ccagacaacg tagccgggct tctggaatac ctccacgacg
     1021 agggtgtgga agtcgcgcat ctcgatgccg tcgacgacga ccgtgtaatg ctcgccgaac
     1081 ttgaaggtga tcagatggag gatggtgaag acgaggatca cgatgcccga ccagatcatc
     1141 gtccgggagg aaagggtctt gcggctgggc ttgccggcgt cggcgatctc gtggtaaccg
     1201 accggccgcg cctcgcgcgc ccggatgtag accagcgtgc cgatgacggc gtggagcaga
     1261 aagaaggcca gcagcaccgc ctcgccgaac cagaggagcg gccccaggct gaccagatga
     1321 tgcgaatagg agttgtagag gttggggttg tccagcagca gcaggaggtt gccgcccaga
     1381 tgcacgatga cgaagacaag gaagaccagg ccggcggccg cggccagata cttcttgccc
     1441 acggtcgtcc acaggtcgca acacgaaaca ggcatggtgg aggctccacg gagggttgaa
     1501 tgggaagcgc ggcgggtctt cgcctcggcg ggcgaggcgc tccgaaggcc gtttcgcggg
     1561 atttccaacg ctggggcgat cccgcacccg caattcatat acccctatct cattggaaaa
     1621 tcaatccccc aagagcgggg ggatttttgg ggcgagggac atacagggac agaaaggacg
     1681 caagggacgg agggcgagag ggcgcgggtg attcggtcag ttgggccggt gggatctttc
     1741 tgcatccctg cgggatgcgg gggtgatggc gatggcgata ccgatagtaa gcttgcatct
     1801 gccgcgagtt tgcaggctaa atgaaggggt cgcgtttcac tccgcccgca gcgccgcgcc
     1861 gagcggcccg cccgcatggc cacagagacc gtcgacacgc acctggaccc cgcgcgctgg
     1921 ctcgatgagc acggcgacct gctctatcgc tacgcgctgg cccgtgtccg cgacgccgaa
     1981 gtggccgagg atctggtcca ggagacgttc gtcgccgcgc tgcgcggcca gcggaatttc
     2041 gccgggcgct cgagcgaacg cacctggctc gtcggcattc tgaaaaacaa gatcgtcgat
     2101 catctgcgcc gcaaatggcg cgaacagccg ctgagcgagc tggagccggc cgagcaggcc
     2161 gaggacttcc tcgatctgct gttcacacgc ggcgggcgct ggaaggaagg ggcaggccgc
     2221 tggggggacg accccgccgc cggagtgcgc gcggccgact tccagcgggt cctcgccgcg
     2281 tgtctcgagg ggctccccgg ccggctggcc gatgttttca tcctgcgcga gattgaacaa
     2341 cttcccagcg atgaagtccg tcaggttctg aacatttcgc cgactaatct gtgggcgatg
     2401 ctgtatcgcg cgcgcatgcg cctgcgcggt tgcctcgaag ccaactggct cggccacgcc
     2461 gaggggaaac gctgacgcat gctgacctgc cgcgatgtga cccgcctcgt ctccgagtcg
     2521 ctcgaccgtg aacttccgcc gctgttgcgc ttccggttaa gactgcacct ggtcctgtgc
     2581 ctgttctgcc gccggttcga gcggcagatg ctcgcactgc gggcgctcgc gcgcgaggtc
     2641 gccgcgggtg aagccgacgg gcccggcctg ccggaggccg cgaaggaacg catccgcggc
     2701 cggctgcgcg gtgagcaggc gcaaccctga gacagcgata caggagaagg aaatccgtcg
     2761 gattggaggg gattcgccgc cccttcgagg ggcttggatc attggaggcg tccacgcagg
     2821 gttccggcct gcggccgtca ccctgcgctt tattccgctc gtccgcttcg cggactcagc
     2881 gggcaaccga accggaaccg ccggcattgg atatggaatc cttgagcgga tcgcgattgg
     2941 acccgcgccg gagcggctgc ggctagagca gccggagcgt atcgatccgc cgcgacggca
     3001 gattcagttc gacggcgccg cggctgacgc gcaacggctc ggccggcgtt tcctccaagt
     3061 cgcagcgcca cgcctgtttc agaccggcgc gatgaaccac gcagttggcc gcgctccgtc
     3121 cggggttgaa caggcgtagg atcgtcccgc ggccatcgtc ggcggccttg acggcgctga
     3181 cgatcacctc gccctccacg cgcaggtagg cgtgggtgcg cggcagcggc gcgccgtcgt
     3241 cgtccacgcg ctcgggatcg gtcggcggca tgtcgggcag gtggtgggcc tggccgatgc
     3301 ccgccgccag ccgctgaccg agccgggtca gcgcgaccgg atcgatcgcg ccggtcagcg
     3361 gcacgatcca gtagcggaag cggtgcgttc cctggatctg gccgccctcc tcgccgcgcg
     3421 tcatcacggt ccgcctgaag gcgcgcagca gcgtgagcgc gatggggcgc tcggggaggt
     3481 cgcgcacggc ggcttccggg aggccgtggc tgacgatcgc gaggccgcgg cggccgtcgg
     3541 agacggcggt ccacgattgc tgcggccggg tctcgacttc cagttcgcgc cactggatgc
     3601 gctccgggtt gagcgcgatc ggccgctcga ccgcgtcgaa gggcgtatcg ctccaggcgg
     3661 tctgcgccga cgcgccggag gggaagagca cgcgcaggcg gtgatcgcgc aggttgtttt
     3721 ccacggtcgt ttcgacctca atgcggtcgc agccggcgcg cagcgtgaca tcgtggcgga
     3781 cgcgcagcgt gcggcgttcg tcgctccggc gcatctcgac aaagtcgaac cgcgccggca
     3841 gggtccattc cagttcgacg cggaagatcg ccttgagcgg accgtcggcg acgagcgcca
     3901 cggtcgccgg cgccgccgag gagacatgca cctgatcgtt gagcggcacg ccatggtacc
     3961 agccgtcgcc gatgtcggcg ccgttttcaa gtgtcaggag tggttcgtag gtctcctcgg
     4021 tgcgcttgtc ggtgacgatc aactggcccg ccagggtgaa gcccagggcg aggaactcgt
     4081 tctcggccga ggtcgggctc gtggccaggc cggtcgtccc gtagcgggtg aagccctcga
     4141 ccgggcgcac cgcgagcgag gtgtagccga agggggggat gcgcagccgg gcggtgcaat
     4201 ggacgtggaa ccgccggggg gatttgggcg gcaggccgcg cgtccagacg atctcgggct
     4261 ggtggaggga ctggccgacg cgctggtagg cgacctcgcg tccgcgctcg tcgtggagcg
     4321 tgaaggccgg ttttttttca aagccgaacc actccatgaa gcagggggta tcctcgggca
     4381 ggacgacgtc gaaatcgacg ggttcgtcga tcggccgcgc ggtcgggttg gccagcagca
     4441 gcgtcgcggg gggatgggcg gcgccggggg ctggcttcag gccgcggtgc acatggcgcg
     4501 ccaggtgccg cagcgccccc tgccggatcc ggtcggcgat cagctcgacc tggtcgaagc
     4561 ggtagagcat gtccttgtgg acctggtcgg ggctgcaacc gcagatgctg tcgtgggcgt
     4621 gattctggag gagcaagcgc caggccgcgc gcaggaaacc ggccgggtcg ggcatgccgc
     4681 tggcggtggt gaacaggccg aagggttcgg cccactgcgt caggagggat tcgcaccgcg
     4741 cgttggcctg cttcagatga atccggctgc tgaggacgcc ggggatcagc cactgctgct
     4801 cgacggaggt ggggggcttg ccgtattcgc gcagctcgcc gtcgatggtt ttggtgatgc
     4861 ggtcggcgga gcgcatcagg tcggccgcgt aggcgtcgag cgtgctcagt ctgacgtcga
     4921 agcgcgccgc ttcggggcgc cgggcgaagt cctcgatcag ctcgcccgtc agcggctcca
     4981 gctccatgtg gtcgcagccg tcgaagaaca ggaccggcag gccggctggt gggcggcccg
     5041 tctgcgtggc gaggtattcg acggccgcgt cggtcaggct ttgccggcgt cggtcggcgg
     5101 gatgcgcgcg gcggcggacg cggaagtaaa aatcgcaata gccgttgttc gcaccgaaac
     5161 ggaaggcgag aacttcggag ccgtccgggg cgcgccagcg gaagttgcgt ccgacgtcgg
     5221 cctcgttgat cccgcgccag aggaaggcgg tcgcgatgcg gaagccgcgg aagatctgcg
     5281 ggagttggct gcaatggccg aagatgtcgc agataaagcc ggcgcgcgag ggctcgccga
     5341 agcggcgcgc cacgagcagg ccctcggcca ggttgcggac gatcgactcg cccgccacga
     5401 tgaattcgtc ggggagagta taccacgggc cgatcgcgag gcggccggcc tgggcgaagc
     5461 ggcggatctg ctcctcgcgc tcgggacgga tctccaggta gtcctcgatc gggatgctct
     5521 ggccgtccat ctggaagtga gtgaaccgcg ggtcgtgctc caggcggtcg agcacctcat
     5581 ccagcagata gaccaggcgg tagcgaaacc cctggaaggg ttcataccat tcacggtccc
     5641 agtgggtgct gattacccag tggagcggga ttttgccgcc gcgggagggg gccggcgcgt
     5701 tccgccccgg tttcgttgcc cgtgccgttt tcgttcgaac catgatgatg gacgtccttt
     5761 tctgtttagt ctgttccgac tgttccgtcc ccgccgggat gacccgagat ctttctcaag
     5821 caagcagatc cagtagttcc ggcatccggt cgatgacgcg gtcgggtcgg agcgcgaggg
     5881 cctggcgccg gctgagcaac ccccaggcga cggcgatcat gcggacgccg gcggcgcgcg
     5941 cgacgcggtg atcgggcagg gcgtcgccga ccatcgcgca gcgatcgggc gggaagccaa
     6001 aatggcggat aacacgcagc agcgactcgg gggcgggctt gcgcgggacc ccctcgagtt
     6061 cgccccacag gccatcgacg cgggcggcga tgcccaggcg gtcgcagacg cccgccacga
     6121 cctcggccgg cttgttcgtc agcacggcct ggcggatgcc gcgcgcgcgg acggcatcga
     6181 gcgtttcgag gacgccggga tagagccgcg ccgagccggc gggattgcgg ccgtagtatt
     6241 cgcggtagcc gcgataaata tcatcgaagc ggtcgagggc gccgacatcg gcgaggcagc
     6301 gccgcacgag catccgggcg ccgtcgccga cgtggccgcg cacctgcgcc atcggcagct
     6361 gcggcagttg atgccggctg aagacggcgt tgatgccgcg cgcgatatcg tcgaatgcgt
     6421 cgacgagcgt gccgtccagg tcgtagacga tcagatcaag cgcgagcggt tggggggagt
     6481 tcatagcgct caacaatcgg catcgggatc gccagcggga tcgtcgtcga accgatggat
     6541 ccgctcgtct cgcgcgccgt cgacagaccg gcatggggcg ggcgcgggca cgacgccccc
     6601 tgatttaacc cgcttgcgcc gcgagaatcc attgaaggtt gcattcaggg gggcggcccc
     6661 tagaatgaag gaacgagccc actccgccct ccgcgccggt ccgccggcga tgccttgcat
     6721 tccagacgga aaggctcctg ttaattccat gacggtcaac cagcccgagc cgatccccga
     6781 aaaagggttt gcgatcaagg actgcgcgct gacggcgatc gcgacgggcc gccgcgcgca
     6841 gaacctgcgc gaactgcgcc acatcctgca ggaaatccat cccggcagca tttattatca
     6901 cttctggggc gggctgctgc ggccgcgcta ctacgatcgc gaatataaga acgatttcgc
     6961 caactgggcc gccacgagcc tgcatcacca gacgctggcc gagcggctga gcgtgattga
     7021 tccgaacgac tacgccgaca tcgagcggct gcgcgaggag ctgctcgaca cgatcgacga
     7081 cgtgctggac gcgacggaac aagtgccttg ggccgtggcc gatgagcagt ttcacttcat
     7141 gcgctcgcag atcgtggtgt tcagcacgca ccatctggtg gccgagccgg aacagctcaa
     7201 ggaggtcgtg ccggcgatga cgatcagcag cgttttttat catttcattg atgcacgcag
     7261 ccggaccgac gcgcacgtgg acgactttcg cgcctggctg ggcgggatgt cgccgcgcta
     7321 tgacgacctg tgcgagcgac tggcgcaagt ggatccgtat tttgaatcgc tcccgcggct
     7381 gcgcgagcaa ctgagcgagc tgctcaacga ttacttcggg ggcggcgcat catgaggtcg
     7441 atgctcgagg catatgccga ggtagccggg gcggacgtca tcgaccagtt gcaccagctc
     7501 gccgagccgc tgcgcggcaa acgcgtggtg catgtcaatt cgacgcggat cggcgggggg
     7561 gtggccgaga ttctgaacaa gctggtgccc gcgctcgagg agctggggat cgaatcgatc
     7621 tgggaggtgc tcaccggcga gagcgatttt taccagtgca cgaagggatt tcacaacgcg
     7681 atccagggga cggcggccga cattccgccg gcgttgctgc gcgtgtatga ggagacgaac
     7741 gcggagaatg ccgagcggct gcggccggtg ctggccgaag ccgacatcgt cctgatccac
     7801 gacccgcagc cggccgcgct gttgcggcat attcccgagc ggcggggcaa gtggatctgg
     7861 cgctgccata tcgacgcgag ccgtccgtac cgcccggtgt ggcgctacct gcgcaagttc
     7921 gtcagcgagt acgacgcgag catcttttcg ctggcggcgt tcgcccagcc gttgccgcac
     7981 aacgtctaca tcatcccgcc aagcatcgac ccgctgaccg agaagaacat ggacctgccg
     8041 gaggaggaga tcgcggcggt gcgcgagcgg ttcgggatcg atccggaccg gccgatgatc
     8101 ctgcaggtgt cgcggttcga ccggttcaag gatccgctgg gggtgattgc ggcgtatcgc
     8161 gaggcgcgca agttcgtgcc caacctgcaa ctggtgctgg cgggcggggg cgcgaccgac
     8221 gatccggagg gcgacgtcgt gctgacggag gtgcagcagg cagcggccgg cgacgccaac
     8281 atcaaggtgc tgctgctgcc ggccgacgcg caccgcacga tcaacgcgtt gcagcgggcg
     8341 gccgacatcg tgctgcagaa atcgctcaag gaagggttcg gcctgaccgt gaccgaggcg
     8401 atgtggaagg gcaagccggt gatcgggggc aataccggcg ggatccggct gcaactggtg
     8461 aatcacgaga ccgggttcct ggtcaatacg ccggagggag cggcgatgcg cattcgctac
     8521 ctgctgttcc accagaagca gcggctgaag atggggcgca acgcgcggga gttcgtgcgc
     8581 gagaactttc tcgtcacgcg gcacatgcgc gagtatctga cgcttatctt gacgctgatc
     8641 cacggcggcg aggaacggat cgtgctgggg taggacggaa ggaggcgacg gtgcatcgcc
     8701 ggcaggaaag gccacggtcc gcccgcgggt ggcatgcggt gatgcggttc gtccggccgc
     8761 tggttgcgca ggcggcgctg ctctgggtgg cggcgtgcgg cggcggcggg gacggcgcgg
     8821 ggcagccgga gatcaccttc gcggcatcgc tgctgcccgc ggaacaggcg aaatacaata
     8881 cgattctgga cgaattcacg cggcaaacgg ggatcccggt caagctggtc gcggttcagt
     8941 acgcccagat ccgctcatcg gtcgaagccg aggcgcaagc ggggcggggc aagctcgatc
     9001 tggtcgaatt ggacctgccg cagttgccgg tgatgcgccc gctgatgcag ccgctcgacg
     9061 gcatcacgtc ggggatcgag gcgctgcgcg acgccgtcgc ggagggggcg tgggaggtga
     9121 cgaccgcccc gggcgccggc ggcgcggagc ggacgctgta tctcccccac cggctgaact
     9181 ggcaggcgat ggtgtatgac agcgagaagc tgcccgagcc gcccggcacg tgggaggaac
     9241 tgctggcggc ggcgcgggcg cacgcggggg cgatcgggct gaagtgcgcg cgctacgagg
     9301 ggctgatctg cgacgtcttc ccgttcatct ggcaggccgg gggcgatccg ctgcgtccgg
     9361 actcggcgga ggtggtccag gcgatcgagt tcttgcggga gctgagcgcg tatttcaatc
     9421 cggccgtgcg gtcgtatcag gagacaccgg tgctcgaagc gcaacggctg ggcgaaatcg
     9481 tgctgcattt caactggccg ttcgtcgtgc cgctgctgcg ggaacagaag ctgctgccgt
     9541 cgccgatgaa ggttgcgccg ctgccggcgg ggccggcggg ccgcgcgacc gtgctggggg
     9601 gcggctatct gggcatcccc gccgcggcgc cgcatcgcga acaggccgcc cgcctgatgg
     9661 aattcctgac ttcagtggag gggcagcggt cgctggtgcg cgagctgggc tggtttccga
     9721 tgcgggagga aggctgggcg gcgatgagcg cggcggaccg tgagctgtat gccggctacc
     9781 tggcgatgcg gcgggaagtg cgggcgcgcc ccgccgtcgc cgactatgac gcgctgagcc
     9841 gccggtggca ggatgcgatt tacgagatcc tgttcgaggg ggcggacgtg aaggggcgat
     9901 tgaaagcgct gcagagcgag attgaggaac cgggtgcgga aggctaaaat aaaacattcc
     9961 ttgcgtcctg tctcacggcg ggatgaagcc agccggcggc cccccgcact ccgtgcccgg
    10021 cgcccgctgg cgaacgcgga aaggtcctcg atgatgatcc gatccctcgt ccttgcctgc
    10081 ctgatcggcg tgatttcgcc aggccatccg gggttcgcgc agccggagca gccgctgcgc
    10141 cccggggagt atcagccgcc ggcggcggcc gatctgatgg cctcatatca gcgcgcgctt
    10201 gcggaggcgc acagttactc gctggacgtg cggcagcgcg tcgtgttgcc gaacctggac
    10261 gtggcgacga cgatggtgat cgccgttgag cgtcccaacc ggctgcttta cgtggcggag
    10321 gaccagccgc cgggaatgtt cgccgggcgc gtggcggccg acggcgagaa actgtatttg
    10381 caccgcccgc agcagaacct ggtggtcgag cgcgaagcgc cggccgacct ggcggagctg
    10441 gccggcgacc ccgagattca gatcctgctg gccaacaacc tggagctggg tctggatctg
    10501 gtgctgccga tgctggccga ggaaccgaca tcgggaccga cgccgtatga atacgtcggg
    10561 catgaaacgt ggagcggccg gctgctgcac cacatccgga accggcgcca gattgatgag
    10621 gggctgtcgc ttcgcttcga ttactacctg accgatgggg acccggcgca gctcgtgcgg
    10681 atccaaccgg attttggcga gctgatgcgg gtgatgatgc gcaatcatgg cgaggacgcg
    10741 gccgcgctgg aggccgcgag cgcgcaggcc gagatgtatt tcgacatcgg gacgtggcag
    10801 ctcaatcccg agctggaggg ggagctgttc gcgttcgcgc cgccggcgac ggccgaggta
    10861 tccgacacgc tgttcgcgca ggagaagcat ccgctggtgg ggagcgaggc gcccgatttc
    10921 gacctgccgc tgctcgacgg gggccaggtg cagctctcgt cgcatgaagg atcgcggatt
    10981 gtgatcctgg atttctgggc gacgtggtgc ggcccgtgcc gcgtggcgat gccgatcatc
    11041 gacaaggtca gcgaggaatt cgcgccgcag ggggtgcagc tgtacgcggt caacctgcgc
    11101 gagacgccgg aaacgatcaa gggattcctg agcggtcagg aatttgcgcc gacggtgttg
    11161 ctcgatgcgg agggaacggt gagcgaccgg ttcggcgtcc agggaattcc cacgacgttt
    11221 atcatcggca aggaggggcg ggtgcaggcg gcctatgtcg gcgcattgcc caacctggaa
    11281 cagcggctgc ggcgcgacct gaagaagctg acgggcggcg aaagcctgct gcccgcgggc
    11341 gccgatgagt gagggccggt cggccgcgac gccggcacac ggagaataat tttctcccct
    11401 gatccgatca gcggggcaaa ttgcacactt tttgcacgcg cggggaccat gaagcattgg
    11461 gcccagcaac ggatgattta tggtctcgcg gccgtgccgg cgttgctgtt cctgctattt
    11521 cagatctggc cgctgggcca tgccatccag acggcgttca ccaacgagac cgggcagtgg
    11581 acgctggagc ctgcgcgacg cgtgtggggg gatccgttgt tccggcgcag cctgggctac
    11641 agtttcctga ttccggtggt gagcgtcgcc ctggaagcgg gggcgggcgt cgcgatggcg
    11701 ctgtggttct atttcggtct gggtcgcgcg cgcgggttct ggcggacgat cgcgctgatc
    11761 ccgttcgccg tgccggagat tgtttattta ctgacgatga agctggtttt tcgccagaac
    11821 ggttatctga acagcctgct gtggcacgtg ggcgggagcg aggcgacggt cggctggctg
    11881 gagccgggca cgccgctgat ggtgggggtg gtgattctgg tcgatgcgtg gcgcgtcacg
    11941 ccgatcgtgt tgctgattgt cctggcggcg ctggagcagg tgcccgagtc gtatctggag
    12001 gcggcgcgga tcgatggggc gtcggtgggg cagatcgcat ggcggattct gatgccgctg
    12061 atcctgccgg cgctgctggt ggcgctggcg ctgcgcgccg tcgatgcgtt ccgcatcttc
    12121 gcgacgccgc tggtgctggt ggggatcgat gcgctgccgg tgctgacgtc ggtcgcctac
    12181 cactatcagg aattcgcgcg cgacggcgcg gccgccaacg tggcggcgct gacgctggcc
    12241 ttcacgctgc tgctgctgac cgggctgacg ctgctgggga tgaagcggag gcgaagctca
    12301 tgagcgcggc cggacgagcg tcgagacgag gaatggcgcc gtcgcgcggc gcgcggcgcg
    12361 ggctgacgtg ggccgggctg gcgctgctgc tgctggtgaa ctgcatcccg ccgctgctgg
    12421 tggtgcggca ggcgttcgcc ccgctgggcg agccgccggg catgccgctg cgcctgctgc
    12481 ccggacggct gtcgctgggc cacctggcgc agttgctcga gacgcagaac ctgctgggcc
    12541 ggatcggcct gagcctgtgg gtggccgtgg cgacgacggc gctggcgctg gcgctggggt
    12601 tccccgccgg gtggggggcc gtgcatctgc ggcgcctggg cgacgtgatg acgcggctgt
    12661 cgctggcgag ccggatcctg ccgccgatcg cggtggccat cccgctgacc gccctgctga
    12721 ttccggtggg cctgtacgac catccattcg gcctggggct ggtggtggcg catctgacgc
    12781 tggggctgcc gttcgcgatc ctgctggcct acgcggcgtt tcgggaagtt ccgggggagt
    12841 tggaggaatc ggcgcgggtc gacggctgca cgccgctggg ggcgttctgg cggatcgcgc
    12901 tgccgtcggt gcgcgggtcg ctcgccggcg cgatgattct ggtttttcta ctctcgtggg
    12961 atgaattcgc ttatgcgctg ctgatccaac tgaccaaccg cacgctgccg ccgctgattt
    13021 actacttcac gagctatggc gacatgggat cggccagcac gctcgcggtg gtaatgctcg
    13081 ttccggcggc gggggtcatt ttcgtgttgc aggggttgtt gacgcgcggg gcgctgaccg
    13141 gcggggtgaa ggagtgaggc ggaaacggaa tttggggcgg ggtttgacat ggagaggaga
    13201 agatggcggg gtatcgagag ccgaggacga gagccgagag ccgaattctg gggcgggtcg
    13261 gtgggggcga ataccaggat gagggcgaga gccgagggac gattttggcg tgaggacgaa
    13321 gacgacgatt gcgccggagc agagcgccga ccggatagat gattttttcc gccggatggc
    13381 ggaacagtcg cggatgggga ctgacccgcg gatggaggga cgcccgcggt gcgttctgtt
    13441 tctggactat gacgggaccc tggcgccgtt tcgtgtcgcg cgtctggagg cgacgccgtt
    13501 tcccggggtc cggcggcggc tggggcggct cctcgccggg cggtcgacgc gggtggtgat
    13561 cgtcagcggg cgaacggtgg acgatctgcg ctacctgctg cggctcgatc cgctccccga
    13621 gctgtggggg gcgcatgggt gggaacatca gccggccggc ggcgaggcgc gcgtccttcc
    13681 cctgccggag gccgcgcgca acggcctgcg ggaagccgag gcgtgggccc gcgccggggg
    13741 gctgggcgaa cggctggagc gcaagccggg ttgcctggcg ttgcactggc ggggggtgtc
    13801 gcccgatgtg cgcgacgcga tgcacgactc ggtgcgcgcg ggctggggcg cgctggcggc
    13861 ggccggcggg ctcgaactga ccgaattcga cggagggatc gaactgcggg tgccggggcg
    13921 cgacaaaggg gatgcggtcc gcgccgtgct ggaaacgatt gagtggagag agggcgtgct
    13981 ggcggccttc ctcggcgacg atcggaccga cgaggacgcg ttcgccgcat tgaagcagag
    14041 cctgggcgag cgagcgttga atgtgctcgt gcgcgatcaa tggcgcgaat cctgcgccga
    14101 tgcctggttg cgaccacccg cgtgcctccg ccgatttctt gatcgctgga tcgcgctgga
    14161 gtgaagcccg aatccgcggt ttgcgcgatt ggcgccggcc cggtataatt ctggaaaacg
    14221 catcgccgaa tgaggcggcc gtgaaggcaa aggaggaggc gcgttttgga cgagacgcga
    14281 cgcggactga ttgttgtttc aaatcgcctg ccgtatgcgc tcagccgaga cgaagcgggc
    14341 cgcctgacgc tgcagccggg ggcggggggc ctcgtcaccg cgctggtgcc ggtgctgcgc
    14401 aaccgcgggg ggacgtggat cgggtggtcg ggaattgaaa gcgacgaccg gcaactgcag
    14461 gccgaggcgg cgcgggtgct ggcgggggcg acgcgcgacg cgggatatca gctccagccg
    14521 gtgccgctga gccatgccga ggtggaactg tattatcacg gctattcgaa cgaaacgatc
    14581 tggccgctat tccacgacct gcagtcgcaa tgccgcttcg atccggagta cgggcgggcc
    14641 tacgacgcag tgaaccggcg gttcgccgag acgatcgcgc gccacgcgcg ggtgggcgac
    14701 ttcatctggg tccatgatta ccagctgatg ctggtcgggc atcatctgcg cgagctgggg
    14761 gtgacggcgc cggtcgtcta ctttcatcac attccgtttc cgtcgatcga cattttcctg
    14821 aagttgccgc gccggtcgta tgtgatccgc gccctgctgg gatacgatca gcttggcttc
    14881 cagaccgcgc gcgacaagcg caatttcctc cagtgcgtgc ggacgctcgt cgacggcgcg
    14941 cgggtccgca gccagggttc gctgcatctg atccgctggg aggggcgcga gatccgggtc
    15001 ggggcgtttc cgatcgggat cgatttcgag gagttcgccg ggcaggccgc gcaggaggag
    15061 gtctcgcgcc gcgcgtggaa catccacgcc gggttgcccg agcggcagat cattctcggc
    15121 gtcgaccggc tggactacac caaggggatc ccgcaccggc tggaggcggt gcgcacggcg
    15181 ctgaagcggc accccgagct gcatcgccgg ctgacgttcg tgcaggtggt ggtgccgagc
    15241 cgcgtggacg tgccgaaata cgcccggctg aaggaagaga tcgagcggct ggtgggcgaa
    15301 atcaacggcg aatttacgca gggggggtgg gtcccgatcc attacgtcta ccgcgcgctg
    15361 ccgcgcgagg aactgctggc ctactaccgc acgagcgaga tcgcgctgat cacgccgctg
    15421 caggacggga tgaacctggt ggccaaggaa tactgcgcgt gcaccctgga agacaacagc
    15481 gtgctgatcc tgagcgagtt tgccgggtcg gcggcgcagc ttcatcgcgg cgcgctgctg
    15541 gtcaatccgt atgatgtgga ggaggtcgcc gacgcgatcc accgcgccta catgatgccg
    15601 agcgaggagc gccgcgcgcg gatgcggcgg atgcgccggg tgatccgcca gcaacacatc
    15661 ttctggtggg tcgatacgtt catgcagtcg gcgatcgcca aggatctgag cgattttccg
    15721 gtgctggagg agtacgactt catccccccc gaacgggtgc atccggagga gcaatcgtcg
    15781 ggctgaagct tcaggcctgg cgcagtccct ggaagcaggc gggcgggagc cgatccagat
    15841 cgggaaaggc catccgggcg atgccgagcg ggcggcgccc gttgaagagc gccaggccgg
    15901 tcatctggag gaagagggcc tccaggcgca gcaggggcag tccgatgacg caggtcagat
    15961 cgccctcgac ctcggtgatg aagtcggcgc cctcgccctg gatgccgtag gcgccggcct
    16021 tgtcgtcggc ctcgcccgag gcgagatagc ggtcgatggc ggccggatcg agctggcgga
    16081 aatggacgcc ggcgacgtcg gcatcgacga gtgtttcgcc gccgacgcgg tgcagcgcga
    16141 ggccggtgat gacttcgtgg cggcggccgg agaggcgcgc gagcatgcgc cgggcctcgt
    16201 cgcggttggc cggcttggtg taggcgccgt cctccaggac gacgagcgta tcggcgccga
    16261 tcacgatcgt gtcgcggggg agatcggggg cgacggccga cgccttggcc atggcggtct
    16321 taagcgcgct ggcgcgcggc gcggaggggt gatggtaggg cgattcatcg agcgccgaga
    16381 cgcggacctc gaaacggacg ccgccgg
//
LOCUS       DAOPCO010000057        16221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3644_length_16221_cov_4.698998, whole genome shotgun sequence.
ACCESSION   DAOPCO010000057 DAOPCO010000000
VERSION     DAOPCO010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16221
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3644_length_16221_cov_4.698998"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..5039
                     /gene="gltB"
                     /locus_tag="PLS90_06180"
     CDS             513..5039
                     /gene="gltB"
                     /locus_tag="PLS90_06180"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HPK02027.1"
                     /translation="MRKQEGLYIPQYEHDACGVGFVTDLKRPASHRIIQQGLQILRNL
                     EHRGACGCDASTGDGAGILMQVPDLFLREVAVEAGFDLPDPGHYGVGMVFLPRKVDEA
                     NYCELAFESVIRRNGLRLVGWRDVPTDNRHIGRVAREGEPAIRQIFIGRPRELKTQED
                     FERRLFVVRKQVEHLVDRSKIAQKPLFHIPSLSSVTLIYKGLLLADQVARYYPDLNDE
                     RMISRLALVHQRYSTNTSPTWDLAQPFRYLAHNGEINTLRGNINWMNARQSQFSTPLI
                     EEIETLFPIIRDGQSDSACLDNALELLFQTGRSLPHCVSMLIPEAWQHHESMVEEKKA
                     YYEYHACLMEPWDGPASIAFTDGRMIGAVLDRNGLRPSRYWATQDGLVVMASEAGVLD
                     IPQEEVTLKGRLQPGRMFLCDLGEERIISDEEIKERLYSRQPYGQWLGENLVRVDELP
                     NPRNFRGFAPETLPVREQLFGYTLEDMNILLAPMAIEGKEPLGSMGNDAALAVLSDKP
                     RLLFDYFKQLFAQVTNPPLDAIREELVTSLNLYLGREDNLFDETPRHARRIKLEQPIL
                     TNSDLERLKRVKTDSHTRSRVFPILFPADDGHKGLRRALRDLCEQISRAIGEGIDIIV
                     LTDRDCGNDMVPIPSLLAVAAIHHHLIREGTRTQASLVLESGEPREVHHFALLFGYGC
                     SAVNPYVALDAIESLRRDGALGEISMEKAKKNYIKAINHGLLKVMSKMGISTLQSYCG
                     AQIFEAIGLHPNVVDRYFTGTASRIEGIGLETIAEEAIRRHQKAFPPRNIPLNLKLDL
                     GGQYQWRRDGEKHMINPFTISKLQEAVRRNSWETFSEFSRLLNEDARMRCTLRGLLTF
                     RKSLQPVHLDEVEPAAEIVKRFCTGAMSFGSISKEAHETLAIAMNTLGGKSNTGEGGE
                     DPERFRDNRNSAIKQVASGRFGVTINYLASARELQIKMAQGAKPGEGGQLPGHKVDEV
                     IGRIRHSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNSNPAARISVKLVAEVGVGTVA
                     AGVAKGKADVVLISGHDGGTGASPLTSIKHAGLPWELGLAETHQTLVMNDLRSRIVVQ
                     TDGQLKTGRDVAIAILLGAEEIGFSTAPLISMGCIMMRKCHLNTCPVGIATQDPVLRA
                     KFSGKPEYVINYFFFVAEELRAIMADLGFRTVDEMIGRVDMLDTADAIDHWKARGLNL
                     APILHKPQVPAHIITRCTMSQDHGLERALDNELIRLAGPALERKERVQLELPIRNTNR
                     TVGTMLSHEIAKRFGLDLLPDDTIHLRFRGSAGQSFGAFAARGLTLEVEGDANDYFGK
                     GLSGARLIIYPSRKARFVAEQNVIIGNVSFYGATSGEAFIRGIAGERFCVRNSGARVV
                     VEGVGDHGCEYMTGGRAIILGQTGRNFAAGMSGGIAYVLDERDTFPARCNKEMVDLER
                     IEAGEEEHFVREMIQRHLDYTGSTVAAAVLADWERVRNLFVKVFPRDYKLALEERARR
                     DEQAAFEQEPVAVK"
     gene            5128..6603
                     /locus_tag="PLS90_06185"
     CDS             5128..6603
                     /locus_tag="PLS90_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HPK02028.1"
                     /translation="MGDIKGFLKYTREEPQDRPVEERVHDWREVTSKPSDVTLGRQAA
                     RCMDCGIPFCHTGCPLGNLIPDWNHLVYRKDWREAIDRLHATNNFPEFTGRICPAPCE
                     AACVLGIIEPPVTIKQIEKAIIDHAWDCGWIEPEPPEIKTGRKVAIVGSGPAGLAAAQ
                     QLRRAGHSVTVFERADRPGGLLMYGIPDFKLEKFQVERRIRQMEAEGVIFRTNCWVGR
                     DVKGDELLREFDAIVLAGGATAARDLPIEGRQLKGIHFAMEFLTQQNKVNQGDAIAPA
                     DRILATDQHVVVIGGGDTGSDCVGTSVRHGAKSVTQIELLPMPPNERTENYPWPYYPM
                     VLRTSSSHEEGCNRQWSVLTKRFTGDDAGNVRLLHCVELEWTNDDSGRMKMTEVAGSD
                     FEIEADLVLLAMGFVGPEKEGLLQQLGVELDERGNVKADEDYRTSVDKVFVAGDMRRG
                     QSLVVWALAEGREAARGVDEYLMGYSDLPTFRTRVDSLPRR"
     gene            complement(6644..7678)
                     /locus_tag="PLS90_06190"
     CDS             complement(6644..7678)
                     /locus_tag="PLS90_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPK02029.1"
                     /translation="MTFTLLVVCFAVALALLFDVINGFHDAANSIATVVSTRVLSPRF
                     AVLWAAFFNFVAMFVFHKARVADTIAKTVKIASDDRAFVYVVLAALVGAIVWDLITWW
                     WGLPTSSSHALIGGLVGAGLAYQGIAIIAWAPVWKTILFIPSAPLIGFVIAFCIMVAV
                     SWIFRRWRPAEVDGFFRKGQLLSAALYSLGHGGNDAQKTMGVIMALLIAAGLMAPDTE
                     LSLTHLNTLWIILSCHLAMAIGTAFGGWRIVKTMGMKITKLKPVGGFCAETAGALTLF
                     LATHGGIPVSTTHTITGSIIGVGAVTKLSGIKWGVAERIVWAWIVTIPFSALVAAACF
                     YLFKLLHPAF"
     gene            complement(7684..8310)
                     /locus_tag="PLS90_06195"
     CDS             complement(7684..8310)
                     /locus_tag="PLS90_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPK02030.1"
                     /translation="MFRSLLPKETGFFDYFEQVSALAVECCRELRQMVSYEGDITAHV
                     DRIKEIEKEADRITHTCTDALHNTFITPIDRAQIHGLSKRLDDVIDAIDATTSRMAIY
                     EMTDVRPEALQLAEVLAEASGEIEQAVKHLRNLRRPEDLNQHLIRIYSLENQGDAILR
                     GALSRLFKEEPNPIQVIKWKEIFERLERATDRCEAVASIIQSILIEAS"
     gene            9021..10559
                     /locus_tag="PLS90_06200"
     CDS             9021..10559
                     /locus_tag="PLS90_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPK02031.1"
                     /translation="MPGAFAKSLSFGVFGIEPYQVTVEVDVTIGQREDSIFQIVGLPE
                     GAVKESRERVRAAIGNSGFWFPAGRVTANLAPADIRKEGVAFDLPLAVGVLAASGSLS
                     PDLLGRYALVGELGLNGEVRPVRGALPLALGARNAGVSGILVPTDNAPEAAVVERVQV
                     IPVATLAEAVEFLAGTRPILPHHPPPFVTENGQHIPDLADVRGQAGARRALEIAAAGA
                     HNILMIGPPGSGKTMLARRLPGILPPMTFEEALDVTKIHSIAGLTGRGGLITNRPFRS
                     PHHTVSHVAMIGGGTLPRPGEASLAHHGVLFLDEFPEFPRQALETLRQPLEDGAVTVA
                     RAAMTCEFPARFMLCASMNPCPCGYRTDPARECRCTPPDVQRYHARLSGPLLDRIDIH
                     IEVPAVKYQELTRGEPGEPTAVVRERVQAARQRQLDRFRGRPGLYANAQISGRDLRRW
                     CRLDDATLAMLERAMGSMGLSARAYDRILRVARTIADMAGKDEIDSEAVSEAIHYRNL
                     DRRL"
     gene            complement(10645..12165)
                     /locus_tag="PLS90_06205"
     CDS             complement(10645..12165)
                     /locus_tag="PLS90_06205"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPK02032.1"
                     /translation="MPLELAYIVAPVILICIVLVASLLDRWSVPVILIALGAGILFGS
                     DVLGLWVFSDFLQANQIANFALMFILFYGGFSTQREHFRQVALPAISLATIGVVLTAL
                     ATFLVLWLLAGWRFEVALLVAVIVSSTDAAATFSILRRHSLPPNLSSTIEIESAANDP
                     MAILLTLGAIEAVSHGQFSTLALVGEFAWKFFGGIGFGIALGHGAVWLFNRLGPEDRG
                     HYYVLSLSVVPLIFGLTNLAEASGMLAAFIAGYVMGNRPFVHRQGVANFFSAMATICD
                     MSMFVLLGLLVSPSQWSGSWLQGVVLFAVLAFIARPFAVWIGTLGMRLGRRARLMIAW
                     AGLRGSVPIVLSTYPMAAGLEFGNEIFNIVFFAVILSVLIQGSTLGNFSSWLGLTTPG
                     RPKPLYNLELMTMSRSDMDLVVVDLPGPQGVQGPRIQNLNLPPGSVITLISRGREVVA
                     PRGDTHLEGWDQVTVLAHVADHEQVRQALVQPFELAMRPPADGEDRDGSPPPAAEA"
     gene            complement(12257..13378)
                     /gene="thiH"
                     /locus_tag="PLS90_06210"
     CDS             complement(12257..13378)
                     /gene="thiH"
                     /locus_tag="PLS90_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HPK02033.1"
                     /translation="MSTFLDIIDRTAWPALGSAIDTATPADVERALAAERPSDDDLIA
                     LFSPAAARYLEPLAQRAAALTERRFGKVIQLYAPVYLTNECVNKCAYCGFSHELEIPR
                     VTLTPARVLAEAEILHAQGFRHILLVAGEDRRAVNLPYLKEVVAALNDRFEAISIEIQ
                     PLEREEYRELVDAGVDGLVIYQEVYDPRLYVKYHLAGPKRNYRKRLRAIEAGGEAGMR
                     TLGVGALLGLAPWRPEAVMVARHARWLMRRFWRARIAVSFPRIRAQAHEYQPDYPVSD
                     ADLVQMICAMRIALPDAELVLSTREPARLRDRLLGLGITRTSAGSSTVPGGYTSHDHA
                     GEQFEIEDSRSPAEVASVIADLGFEPVWKDFDRMFVLAR"
     gene            complement(13474..14256)
                     /locus_tag="PLS90_06215"
     CDS             complement(13474..14256)
                     /locus_tag="PLS90_06215"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HPK02034.1"
                     /translation="MKPLTINGVALRSRLLVGTGKFSSPQAMAAAVEASGTELVTVAL
                     RRVDLNNPHDATLEALDFSKYRLLPNTSGARTAEEAVRLAKMARAATRTNWVKLEVTP
                     DPNYLLPDPVETLKAAEVLVAGGFVVLPYINADPILARRLEDVGCATVMPLGSPIGSN
                     RGLRTRDQLGIIIEQATVPVVVDAGLGMPSHAAEAIEMGADAVLINTAIAVAADPVRM
                     ARAFKAAVEAAELAREAGPGGESRLARASSPLTGFLWEQGSA"
     gene            complement(14280..14810)
                     /locus_tag="PLS90_06220"
     CDS             complement(14280..14810)
                     /locus_tag="PLS90_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPK02035.1"
                     /translation="MRSPTLQAHFRLIQSQRTAIYERLAGCSREELWAPLAPGKWSRG
                     EHLAHLARTLELIRKIARIAIPLLTPFAWLFRNRPFERHSPDVYAEYEQKHGRPMKAP
                     SLIVPARRPEMDLDPLRAELQRQTVKLEALLVRVPEEIAGHIRFLDPPGHNPNLIQAV
                     HILAIHEAHHFKAMFP"
     gene            complement(14822..15025)
                     /gene="thiS"
                     /locus_tag="PLS90_06225"
     CDS             complement(14822..15025)
                     /gene="thiS"
                     /locus_tag="PLS90_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013460460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPK02036.1"
                     /translation="MMRIRVNGESREVDQASSLLDLLRQIGLEARNLAIEHNGEFVED
                     DRLGEVTLNEGDTLEIVRFVGGG"
     gene            complement(15022..15933)
                     /gene="thiF"
                     /locus_tag="PLS90_06230"
     CDS             complement(15022..15933)
                     /gene="thiF"
                     /locus_tag="PLS90_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HPK02037.1"
                     /translation="MKPPVQFPLEIGTEGPRADAEGLPLRVNERPHRVAPGVTLFQLR
                     DRLKPDADVVILNGFPLAEDRPLAAGDEVVLIRRGEVPSPEELESLMVARHGPGVHAR
                     VKAARVGIAGCGGLGSAVAIALARTGVGHLLLVDFDVVEPSNLNRQQFFIDQIGLPKV
                     DALRDNLLRMNPYVRIETRNLRLTARNIPETFAGCHVIAECFDDPTMKRDLTVAVRRG
                     LPTIPLVTVSGIAGYGPADSIRVRRIFRNVWLVGDTETAAAPGRGLLAPRVAVAAGQQ
                     ANVVLRLLLGEEGEGSGRRESDPVEEG"
     regulatory      complement(15926..16033)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         2901 a         5426 c         5334 g         2560 t
ORIGIN      
        1 tccgaggcgc cgacccaacc ggggtgtgga ccgcgcgaaa tccttttcgc agcatgctca
       61 tcgccatcgc ggcggccgcc caagtcgttc ctttaatcat tgataataaa taacttagat
      121 tcgtatctga aatgggctga atgatgcctc gcgcccccag gcgcgccccg attgcgccct
      181 ccgccggaat gccggttggc cgtcgccgct ggttttttcc ttcgacacct ccccggtttc
      241 gtgtgtaaat tttatcggga actcgtggag ggttcccatc cctgttgccc cccttcgcat
      301 tggtcgtcga cccgaccaaa agctgaaccg aggcggcgcc ccccacttcc cattcccatg
      361 ggggtgcatg ggcacccagg ggaagacggc aaccttgagg caggacggcg acgccccgcc
      421 gtggcggatc gcgggcggcc ggcggcaacc cggccctgct gattcgatcc atcgccgggt
      481 ggatcgtgcg gcatcagcga aaggagtctc ccgtgcggaa gcaggaaggc ctatacatcc
      541 ctcagtatga acacgatgcg tgcggcgtgg gtttcgtcac cgacctgaag cgtcccgcca
      601 gccatcggat catccagcag ggcttgcaga tcctgcgcaa cctggagcat cggggcgcct
      661 gcggctgcga tgcctcgacg ggcgacggcg ccggcatcct gatgcaggtg cccgacctgt
      721 ttctgcgcga agtggcggtc gaggcgggct tcgacctgcc cgaccccggc cattacggcg
      781 tcggcatggt ttttctgccc cgcaaggtgg acgaagccaa ctactgcgag ctggcctttg
      841 agagcgtcat ccggcgcaac ggcctgcggc tggtcggctg gcgcgacgtt ccaaccgaca
      901 accggcatat cggccgcgtg gcgcgcgaag gcgaacccgc catccgtcag atcttcatcg
      961 gccggccccg cgagctgaag acccaggagg acttcgaacg gcggctgttc gtcgtccgca
     1021 agcaggtcga gcacctggtg gaccgctcca agatcgcgca gaagccgctc ttccatatcc
     1081 ccagcctctc ctcggtgacg ctgatctaca aggggctgct gctggccgac caggtcgcgc
     1141 gctactaccc cgacctgaac gatgaacgga tgatcagccg gctggcgctg gtgcatcaac
     1201 gctactcgac caacacgtcg cctacctggg acctggcgca gccgttccgc tacctggccc
     1261 acaacggcga gatcaacacg ctgcgcggca acatcaactg gatgaacgcg cgccagtcgc
     1321 agttcagcac gccgttgatc gaggagatcg agacgctctt tccgatcatc cgcgacggcc
     1381 agagcgactc ggcctgcctc gacaacgcgc tggaactgct gtttcagacc gggcgcagcc
     1441 tgccgcattg cgtctcgatg ctgatccccg aggcctggca gcaccacgag tcgatggtgg
     1501 aggagaagaa ggcgtactac gaataccacg cctgcctgat ggagccgtgg gacgggccgg
     1561 cgtcgatcgc gttcaccgac ggacggatga tcggcgcggt gctcgaccgc aacggcctgc
     1621 gcccgtcgcg ctactgggcc acgcaggatg gcctggtggt aatggcctct gaggcgggcg
     1681 tgctggacat cccgcaggaa gaggtcacgc tgaaggggcg cctgcagccg ggtcgcatgt
     1741 tcctctgcga cctgggagaa gagcggatca tctccgacga ggaaatcaag gaacgcctct
     1801 actcgcgcca gccgtacggt cagtggctgg gcgagaacct ggtgcgggtc gacgaactgc
     1861 ccaacccgcg caacttccgc ggcttcgccc ccgaaaccct ccccgttcgc gagcagcttt
     1921 tcggctacac gctggaggac atgaacatcc tgctggcgcc gatggcgatc gaaggcaagg
     1981 agccgctcgg ctcgatgggg aacgacgccg ccctggcggt cctctccgac aaaccgcgcc
     2041 tgctgttcga ttacttcaag cagcttttcg cgcaggtgac caatccgccg ctcgacgcga
     2101 tccgcgagga actcgtcacc tcgctcaatc tctacctggg gcgcgaagac aacctgttcg
     2161 acgagactcc ccggcatgcc cgccggatca agttggaaca gccgatcctg accaacagcg
     2221 acctggagcg cctcaagcgc gtcaagaccg attcacacac ccgctcgcgg gtctttccaa
     2281 ttctcttccc cgccgacgac ggccacaagg ggctgcgccg ggcgctgcgc gacctctgcg
     2341 agcagatcag ccgcgccatc ggcgaaggga tcgacatcat cgtcctgacc gaccgcgact
     2401 gcggcaacga catggtgccg atcccgagcc tgctggccgt cgccgcgatc catcaccacc
     2461 tgatccgcga gggaacgcgc acgcaggcca gtcttgtcct ggaatcgggc gaaccgcgcg
     2521 aggtgcatca tttcgccctg ttgttcggct atggctgcag cgccgtcaac ccgtatgtcg
     2581 cactcgatgc catcgagtcg ctgcggcgcg acggcgccct gggcgagatc tcgatggaga
     2641 aggccaagaa aaactacatc aaggcgatca atcacggcct gctcaaggtg atgtccaaaa
     2701 tgggcatctc gacgctccag agctactgcg gcgcccagat cttcgaggcc atcggcctgc
     2761 atcccaacgt cgtcgaccgc tacttcaccg gcacggcctc ccgcatcgag gggatcgggc
     2821 tggaaacgat cgccgaggag gcgatccgcc gccaccagaa ggccttcccg ccgcgcaata
     2881 tcccgctcaa tctgaagctc gacctgggtg gtcagtacca gtggcggcgc gacggcgaaa
     2941 agcacatgat caacccgttc acgatcagca agctgcagga agcggtgcgc cgcaactcgt
     3001 gggagacctt cagcgagttc agccgcctgc tcaacgagga cgcgcgcatg cgctgcacgc
     3061 tgcgcggcct gctgaccttc cgcaagagcc tccagccggt gcatctggac gaggtcgaac
     3121 cggccgccga gatcgtcaaa cgcttctgca cgggcgccat gtcgttcggc tcgatcagca
     3181 aggaagcgca cgaaacgctg gccatcgcga tgaacacgct ggggggcaag agcaacaccg
     3241 gcgagggggg cgaggatccc gagcgcttcc gggacaaccg caacagcgcc atcaagcagg
     3301 tcgcgtcggg gcgtttcggc gtcacgatca actacctggc gagcgcgcgc gaactgcaga
     3361 tcaagatggc gcagggggcg aagcccggcg agggcggcca gcttccgggc cacaaggtgg
     3421 atgaggtgat cggccggatc cgccattcca cccccggcgt gggactgatt tccccgccgc
     3481 cgcaccacga catctattcg atcgaggacc tggcccagct catccacgat ttgaaaaaca
     3541 gcaaccccgc ggcgcgcatc agcgtcaagc tcgtcgccga ggtcggcgtc ggcacggtcg
     3601 cggccggcgt cgccaagggc aaggccgacg tcgtcctcat cagcggccac gacggcggca
     3661 ccggcgcctc gccgctgacc tcgatcaagc atgccggcct gccgtgggaa ctgggcctgg
     3721 ccgagaccca tcagaccctg gtgatgaacg acctgcgcag ccggatcgtc gtgcagaccg
     3781 acggtcagct caagaccggc cgcgacgtgg cgatcgcgat cctgctcggc gccgaggaga
     3841 tcggcttctc caccgcgccg ctcatctcga tgggctgcat catgatgcgc aagtgccacc
     3901 tcaacacctg cccggtcggc atcgccacgc aggacccggt gctgcgcgcc aaattcagcg
     3961 gcaagccgga atacgtcatc aactacttct tcttcgtggc ggaggaactg agggcgatca
     4021 tggccgacct cggcttccgc accgtcgatg aaatgatcgg ccgcgtcgac atgctcgaca
     4081 ccgccgacgc gatcgaccat tggaaggcgc gcggcctgaa cctggccccg atcctgcaca
     4141 agccgcaggt ccccgcccac atcatcaccc gctgcacgat gagccaggat cacgggctgg
     4201 agcgcgcact cgacaacgag ctgatccggc tcgccggacc ggcgctggag cggaaggaac
     4261 gcgttcaact ggaactgccg atccgcaaca ccaaccggac cgtcggcacg atgctctcgc
     4321 acgagatcgc caagcgcttt ggactggacc tgcttcccga cgacacgatt cacctgcgct
     4381 tccgcggatc ggccggccag agcttcggcg cgttcgccgc caggggcctg acgctcgaag
     4441 tcgaaggcga cgccaacgac tacttcggca agggcctctc cggcgcccga ctgatcattt
     4501 atccctcgcg caaggcgcgg ttcgttgccg agcagaacgt gatcatcggc aacgtgtcgt
     4561 tctacggcgc caccagcggc gaagcgttta tccgcggcat tgccggggaa cgcttctgcg
     4621 tgcgcaacag cggcgctcgc gttgtcgtcg agggcgttgg cgatcacggc tgcgaataca
     4681 tgaccggcgg ccgcgccatc atcctcggcc agaccgggcg caacttcgcc gccggcatgt
     4741 cgggcggcat cgcctatgtg ctcgatgagc gcgacacctt ccccgcccgc tgcaataagg
     4801 agatggtcga tctggaacgg atcgaggccg gtgaggagga gcacttcgtc cgtgaaatga
     4861 tccagcgcca tctggactac accggctcga cggtggctgc ggccgtcctc gccgactggg
     4921 agcgggtgcg caacctgttc gtcaaggtct tcccgcgtga ttacaagctg gcgctggagg
     4981 aacgcgcgcg gcgcgacgag caggcagcat ttgagcagga accggtcgcc gtcaagtgag
     5041 gcgcaccggc gggggagcag gcggagcgga gggctcgtct ccgccgatcg gaaaccagca
     5101 gcacaccaca gcggagggaa acgctcgatg ggcgacatca agggattctt gaaatatacg
     5161 cgcgaggaac cgcaggaccg gcccgtcgaa gagcgggtgc acgattggcg cgaggtcacc
     5221 agcaaaccgt cggacgtcac gctggggcgc caggccgcgc gttgcatgga ttgcgggatc
     5281 cccttctgcc acaccggctg cccgctgggc aacctgatcc ccgattggaa ccacctggtt
     5341 tatcgtaagg attggcgcga ggcgatcgac cggctgcacg cgacgaataa tttccccgag
     5401 ttcaccggcc ggatctgccc cgccccgtgc gaggcggcct gcgtgctggg aatcatcgag
     5461 ccgccggtca cgatcaagca gatcgaaaag gccatcatcg accacgcctg ggactgcggc
     5521 tggatcgagc cggagccgcc ggagatcaag accggccgca aggtggcgat cgtcggctcg
     5581 ggcccggcgg gtctggccgc cgcccagcaa ctgcggcgcg ctggccacag cgtcaccgtc
     5641 ttcgaacgcg ccgatcgccc cggcggactc ctgatgtacg gcatccccga tttcaaactg
     5701 gagaaatttc aggtcgaacg ccgcatccgt cagatggagg ccgagggggt catcttccgc
     5761 accaactgct gggtcggccg cgacgtgaag ggcgacgagc tgctgcgcga attcgacgcc
     5821 atcgtgctgg ccggcggggc caccgccgcc cgcgacttac cgatcgaagg ccgccagctc
     5881 aagggcatcc acttcgccat ggagttcctg acgcagcaga acaaggtcaa tcagggcgac
     5941 gccatcgccc ccgccgatcg catcctcgcc accgaccagc atgtcgtcgt gatcggcggc
     6001 ggcgacaccg gctcggactg cgtcggcacc tccgtgcgcc atggggcgaa gtcggtcacg
     6061 cagatcgagc tgctgccgat gccgcccaac gagcggacgg agaactaccc ctggccctat
     6121 tacccgatgg tcctgcgcac ctcctcgtcg catgaggaag gctgcaatcg ccagtggagc
     6181 gtgctgacca aacgcttcac gggcgacgat gcaggcaacg tcaggctgct ccattgcgtg
     6241 gaactggagt ggacgaacga cgactccggc cggatgaaaa tgaccgaagt cgccggcagc
     6301 gattttgaaa tcgaagccga cctggtcctg ctggcgatgg ggtttgtcgg tcctgagaaa
     6361 gaggggctgc tccaacagct cggggtcgag cttgacgagc gcggcaacgt gaaggcggat
     6421 gaggactacc ggacctcggt ggacaaggtc ttcgttgcgg gcgacatgcg ccgcggccag
     6481 tcgctcgtcg tctgggccct ggccgaaggc cgggaggccg cccgcggcgt cgacgaatat
     6541 ctgatgggtt acagcgacct gcccacattc cgcacgcgcg ttgacagctt gccgcggcgc
     6601 tgaaccgctt cgccgccggc ggcccggccc catgtcggtc tcgtcagaac gccgggtgga
     6661 gcagtttgaa cagatagaag caggcggcgg cgacgagcgc ggaaaacggg atcgtcacga
     6721 tccaggccca gacgatccgt tcggcgaccc cccatttgat gcccgaaagt ttggtcacgg
     6781 cgccgacgcc gatgatcgag ccggtgattg tgtgcgtggt ggagacgggg atgccgccgt
     6841 gggtggcgag gaacagcgtc aatgcgccgg ccgtctcggc gcaaaacccg ccgaccggtt
     6901 tgagtttggt gattttcatc cccattgtct tgacaatccg ccagccgccg aacgccgtgc
     6961 cgatcgccat cgccaggtgg cacgacagga tgatccacag cgtgttcaaa tgcgtgagcg
     7021 agagctcggt gtcgggcgcc atcagaccgg cggcgatcag cagcgccatg atgacgccca
     7081 tcgttttctg cgcatcgttg ccgccgtggc ccagtgaata gagcgcggcc gagagcaatt
     7141 gccccttgcg gaagaagccg tcgacctcgg ccggccgcca gcgccggaag atccaactga
     7201 cggcgaccat aatgcagaac gcgatcacaa aaccgatcag cggcgccgac gggatgaaca
     7261 ggatggtctt ccagacggga gcccaggcga tgatcgcgat gccctggtag gccaggccgg
     7321 cgccgaccag cccgccgatc agcgcgtgcg acgaactggt cggcaggccc caccaccagg
     7381 tgatcagatc ccacacgatg gcgccgacca gcgcggccag gacaacgtag acgaacgctc
     7441 tgtcgtccga ggcgatcttg accgtcttgg cgatcgtgtc ggcaacgcgc gccttgtgga
     7501 agacgaacat cgcgacgaag ttgaagaacg ccgcccacaa cacggcgaag cgcggcgaaa
     7561 ggacgcgcgt cgaaacgacg gtggcaatgg aattggccgc gtcgtgaaat ccgttgatga
     7621 catcgaacag cagcgccagc gcgacagcga aacagacgac aagcagcgtg aaggtcatgg
     7681 ggatcaggat gcctcgatca ggatgctctg gatgatgctg gccaccgctt cgcagcggtc
     7741 ggtcgcccgc tccagccgct caaagatctc cttccacttg atcacctgga tggggttggg
     7801 ctcctccttg aacagacggc tgagagcccc gcgcaggatg gcgtcgccct ggttctccag
     7861 ggaatagatg cggatcaggt gctgattgag gtcctcgggc cgacgcaggt tgcgcaggtg
     7921 cttgacggcc tgctcgatct cgccggaggc ctcggccagg acctcggcca gttgcagcgc
     7981 ctcgggccgc acgtcggtca tctcgtagat cgccatgcgc gacgtggtgg cgtcgatggc
     8041 gtcgatgacg tcgtccaggc gcttgctcag gccgtggatc tgggcccggt cgatcggcgt
     8101 gatgaatgtg ttgtgcagtg cgtcggtgca ggtgtgcgtg atgcggtcgg cctctttctc
     8161 gatctccttg atccggtcga cgtgcgccgt gatgtctccc tcgtaggaga ccatctggcg
     8221 cagctcgcgg cagcattcca ccgccagcgc gctgacctgt tcgaagtaat cgaagaagcc
     8281 ggtttccttg ggtagcaggc tgcggaacat ggtactcccg gacgtcggcg gcggcagcgg
     8341 tccgcgggcc ggacggggag cggagccggt ctcggtgctc cccggcgcgg gtggcggttc
     8401 cgtcagggtg cgcggcgcga cggcgctatc tctgccagac tcggcacccc ccacccccgg
     8461 ctggggagac gccggcaacc aaataaccta acgaacggcc gggcagaagt gaagcggagt
     8521 tgcgcgctca gagtgggcga aattgggggc gagatcaagt gggcaagatc aggcgggcaa
     8581 gggacataaa ggacttaaag gacataaaaa cagagaactg gtggtagttc atcatttgcg
     8641 gatgctacgg cgagtctgtc ttatgctttc atggaggtcc ccttcgctca aaaaatgtcc
     8701 gctgcaactg atgaagttgc cggtccaggc tgaccatggt cggcacggtc acagccggcg
     8761 cgttgcaagg cagcggccgg gcagcggaat atgagggtct gacgctcagc ctcaaacccc
     8821 atgaactgca tcgccgcaaa ccgcctgcgg atttcgaagg gcgatttgcg ggcgctcaaa
     8881 tattaccggt ttctctgaac tcagcatcac ccctttgatg atctagcggt catcatcctc
     8941 tccgccccgt tatcgtctgt tgattcctcc cctcgccttc cttaccatca actcatttga
     9001 ccgaccggac cgacggccgt atgcccggcg cctttgccaa gtccctctcc ttcggcgtct
     9061 tcgggattga gccgtatcag gtcaccgttg aggtcgatgt cacgatcggc cagcgcgagg
     9121 actcgatctt ccagatcgtc ggcctgcctg aaggcgccgt gaaggaatcg cgcgaacggg
     9181 tccgcgccgc catcggcaat tccggtttct ggttcccggc gggccgcgtc accgccaacc
     9241 tggccccggc cgacatccgc aaggaagggg tcgcgttcga cctccccctg gccgtcggcg
     9301 tgctggccgc ctcgggcagc ctcagccccg acctgctggg ccgctacgcg ctggtgggcg
     9361 agctcgggct gaatggcgag gtccgcccgg tccgcggcgc gttgccgctg gcgctcggcg
     9421 cgcgcaacgc cggcgtgtcc ggcatcctgg tccccaccga caatgccccg gaggccgccg
     9481 tggtcgaacg ggtccaggta atcccggtgg cgacgctggc cgaggcggtc gagttcctcg
     9541 ccgggacgcg gccgatcctg ccccatcacc cgccgccgtt cgtcacggaa aacgggcaac
     9601 acatcccgga cctggccgac gtgcgcggcc aggcgggagc gcgccgcgcg ctggagatcg
     9661 cggccgccgg tgcgcacaac atcctgatga tcggcccgcc gggctcgggc aagacgatgc
     9721 tggcgcgccg gctgccgggc atcctgccgc cgatgacgtt cgaggaagcg ctcgacgtga
     9781 ccaagatcca ttcgatcgcc ggactgaccg gccgcggcgg gctcatcacc aaccggccgt
     9841 tccgctcgcc gcatcatacc gtcagccacg tggcgatgat cggcgggggc accctgccgc
     9901 ggccggggga agcctcgctg gcgcatcacg gcgtgctgtt cctggacgag ttccccgaat
     9961 tcccgcgcca ggcgctggag acgctgcgcc agccgctgga ggacggcgcg gtgacggtgg
    10021 cgcgcgcggc gatgacctgc gagttccccg cgcggttcat gctctgcgcc tcgatgaacc
    10081 cgtgcccctg cggctaccgc accgacccgg cgcgcgagtg ccgctgcacg ccccccgacg
    10141 tccagcgcta tcacgcgcgg ctgtcggggc cgctgctcga ccgcatcgac attcacatcg
    10201 aggtgccggc ggtcaaatac caggaactga cccgcggcga gccgggcgag ccgacggccg
    10261 tcgtccgcga gcgcgtccag gcggcgcggc agcggcaact ggaccggttt cgcgggcgcc
    10321 cgggcctcta cgccaacgcg cagatcagcg ggcgcgacct gcgccgctgg tgccggctcg
    10381 acgacgccac gctggctatg ctcgagcgcg cgatgggctc gatggggctg tcggcgcgcg
    10441 cctacgaccg catcctgcgc gtggcgcgga cgatcgccga catggcgggc aaggacgaga
    10501 ttgacagcga agccgtcagt gaggccattc actaccgcaa tctggaccgg cgattgtgac
    10561 ggagcgcccg atgggcggcg ggggcggctc gatcgcgagg gcgatcccga taccgatacc
    10621 gactccatgg gcgatgccag ccggctacgc ctcggcggcg gggggcggcg aaccgtcgcg
    10681 atcctcccca tcggctgggg ggcgcatcgc caactcgaac ggctgcacga gcgcctgacg
    10741 cacctgctcg tgatcggcca catgcgccag caccgtgacc tgatcccagc cttccagatg
    10801 ggtgtcgccg cgcggggcga cgacctcgcg tccgcggctg atcagcgtga tgaccgagcc
    10861 ggggggaaga ttcagattct ggatgcgcgg gccctgcact ccctgggggc cgggcagatc
    10921 gacgacgacc agatccatat cgctgcgcga catcgtcatc agttcgagat tatagagcgg
    10981 tttgggccgg cccggcgtgg tcaggccgag ccagctcgag aagttgccca gtgttgagcc
    11041 ctggatcagg accgacagga tcacggcgaa gaagacgatg ttgaagattt cgttgccgaa
    11101 ctccaggccg gcggccatcg ggtaagtcga caggacgatc ggcaccgaac cgcgcaggcc
    11161 ggcccacgcg atcatcagcc gcgccctgcg ccccagtcgc atccccagcg taccgatcca
    11221 gacggcgaag ggccgggcaa tgaacgcgag gacggcgaac agcacgaccc cctggagcca
    11281 tgagccggac cactggctgg gcgagaccag cagcccgagc agaacgaaca tcgacatgtc
    11341 acaaatcgtc gccatcgcgc tgaagaaatt ggcgacgccc tggcggtgga cgaacgggcg
    11401 attccccatg acataaccgg cgatgaacgc cgccagcatg ccgctggcct cggccagatt
    11461 cgtcagccca aagatcagcg gcacgacgct gagcgagagc acgtagtagt gaccgcggtc
    11521 ctcggggccg agccggttga acagccagac agcgccgtgt cccagcgcga tgccgaagcc
    11581 gatcccgccg aagaatttcc aggcgaactc accgaccagc gccagggtgc tgaactgccc
    11641 atgcgacacc gcttcgatcg cgccgagggt cagcaggatc gccatcgggt cattggcggc
    11701 gctttcgatc tcgatcgtcg aggagagatt tggcggcagc gaatggcgcc gcaggatcga
    11761 aaatgtggcc gcggcatccg tggatgaaac gatgaccgcg acgagcaggg cgacctcgaa
    11821 gcgccagccg gccaggagcc acaagaccag gaacgtcgcc agcgcggtca ggacgacgcc
    11881 gatcgtcgcc agactgatgg ccggcagcgc cacctggcgg aagtgttcgc gctgcgtgct
    11941 gaacccacca taaaacagga tgaacatcag ggcgaagttg gcgatctggt tggcctggag
    12001 gaaatcggaa aagacccaca gcccaaggac gtcgctgccg aacagaatcc ccgcgccgag
    12061 cgcgatcagg atgaccggca cgctccagcg atcaagcagc gacgccacca ggacgatgca
    12121 gatcaggatg acgggggcga cgatgtaagc cagttcgagc ggcacgcgga ttctcccgcc
    12181 ggggattgac gcggacatca tcccagccgg cggaacacgg atcaacccat accggcgcgc
    12241 gaccgcggcc gggcggtcaa cgcgccagaa cgaacatccg gtcaaagtcc ttccagacgg
    12301 gctcgaaacc caggtcggca atgaccgacg ccacctcggc gggggaacgg ctgtcctcga
    12361 tttcgaattg ctcgccggcg tggtcgtgcg aggtgtagcc gcccgggacg gtgctgctgc
    12421 cggcgctggt gcgggtgatg ccgaggccca gcaggcgatc gcgcaggcgg gccggttcgc
    12481 gcgtcgaaag gacgagttcg gcatcgggca gcgcgatccg catcgcgcag atcatctgga
    12541 ccagatcggc atcgctcacc gggtagtccg gctgatactc gtgcgcctgg gcgcggatgc
    12601 gggggaagct gacggcgatg cgggcgcgcc agaaccggcg catcaaccag cgggcatggc
    12661 gagccaccat gacggcttcg ggacgccagg gggcgaggcc cagcagggcg ccgacgccga
    12721 gcgtccgcat gccggcttcc ccgcccgcct cgatcgcccg caggcgcttg cggtaattcc
    12781 gtttagggcc tgccaggtgg tacttcacgt aaagccgggg atcgtagact tcctgataga
    12841 tcacgaggcc gtccacgccg gcgtcgacca gttcgcggta ttcctccctt tcgagcggct
    12901 ggatttcgat tgagatcgcc tcgaaacggt cattgagcgc cgccacgact tccttcaaat
    12961 acggcaggtt gacggcgcgg cgatcctcgc ccgccacgag caggatgtga cggaagccct
    13021 gggcatggag aatctcggcc tcggccagga cgcgcgccgg cgtcagcgtg acgcgcggga
    13081 tctccagctc atgcgaaaag ccgcagtaag cgcacttgtt cacacactca ttggtcaggt
    13141 acaccggcgc gtagagctgg atgaccttgc cgaagcgccg ctcggtaagg gcggcggcgc
    13201 gctgcgccag cggctccaga taccgcgcgg cggccggcga aaagagggca atcaggtcgt
    13261 cgtcggaggg ccgttcggcg gcgagcgcgc gttcgacgtc ggcgggcgtc gccgtgtcga
    13321 tcgccgaacc cagcgcgggc cacgcggtgc ggtcgatgat gtcaaggaag gtgctcatgc
    13381 cgtcacgtcc aggaattcgc ctctcgtttc tcgtcctcgc gctcgccgtc ggacctcaag
    13441 gacataaggg ccataaaggt tgtagcgaag ccatcaagca ctcccctgct cccacagaaa
    13501 cccggtcagc gggctgctgg cgcgggccag ccggctttcg ccgccggggc cggcctcgcg
    13561 ggccagttcg gcggcctcca cggccgcttt gaacgcgcgc gccatccgga ccggatcggc
    13621 cgcgacggcg atcgccgtgt tgatcaggac cgcatcggcg cccatctcaa tcgcctcggc
    13681 ggcgtggctc ggcatgccca ggccggcgtc caccacgacc ggcacggtcg cctgctcaat
    13741 gatgatgccg agctggtcgc gcgtccgcag cccgcgattg ctgccgatgg gcgaacccag
    13801 cggcatcacc gtggcgcagc cgacgtcctc cagccgcctg gccagaatcg gatcggcgtt
    13861 gatgtagggc agcacgacga accccccggc caccagcacc tcggccgcct tgagcgtttc
    13921 cactggatcg ggcagcagat agttggggtc gggggtgacc tccagcttga cccagttggt
    13981 acgggtggcg gcacgggcca ttttggccag gcggacggct tcctcggcgg tgcgcgcgcc
    14041 cgaggtgttg ggcagcaggc ggtatttgct gaagtcgagc gcctccagcg tggcgtcgtg
    14101 cgggttgttc agatcgacgc ggcgcaacgc cacggtcacc agttcggttc ccgaagcttc
    14161 cacggcggcc gccatcgcct ggggcgaact gaatttgccg gtgccgacca acaggcgcga
    14221 gcgcagcgcg acgccgttga tcgtcagggg cttcattgga ctgttcgtgc cgtcggtggt
    14281 catggaaaca tcgccttgaa atggtgggct tcgtgaatcg ccaggatgtg cacggcctgg
    14341 atcaggttcg gattgtggcc cggcggatcg aggaagcgga tgtgcccggc gatctcctcc
    14401 ggcacgcgga ccagcagagc ctccagcttc acagtttgcc gctgcagttc ggcgcgcaac
    14461 gggtcgaggt ccatttcggg gcgccgcgcg ggcacgatca ggctgggcgc cttcatcggc
    14521 cggccatgct tctgttcata ctcggcatag acatcgggcg aatggcgttc gaacgggcga
    14581 ttgcggaaga gccaggcaaa cggcgtcagc agcgggatcg caatgcgcgc gatcttgcgg
    14641 atcagctcga gggtccgggc cagatgcgcc aggtgttcgc cccgggacca cttgccgggg
    14701 gcaagcggcg cccacagttc ctcgcgcgaa caaccggcga gccgctcgta aatcgcggtg
    14761 cgttgcgact gaatcagccg gaaatgggcc tggagcgtgg gcgagcgcat ctccatcggc
    14821 atcacccgcc gccgacgaac cggacgatct cgagcgtatc gccctcgttc agcgtcacct
    14881 cgccgagccg gtcatcctcg acgaactcgc cgttgtgttc gatggcgaga ttgcgcgcct
    14941 ccaggccgat ctgccgcagc agatccagca gggaagaagc ctggtcaacc tcgcgcgatt
    15001 cgccgttgac tcgaatccgc atcatccttc ctccaccggg tcggattccc gccgcccgct
    15061 cccctcgcct tcctcgccga gcagcaggcg caacaccacg ttggcctgct gaccggcggc
    15121 gaccgccaca cgcggcgcca gcaggccgcg gcccggcgcc gcggccgtct ccgtatcgcc
    15181 caccagccag acgttgcgga agatgcgccg cacccggatc gaatcggctg ggccgtaccc
    15241 cgcgatgccc gatacggtga cgagcgggat cgtcggcagg ccgcggcgca cggcgaccgt
    15301 cagatcgcgc ttcatcgtcg ggtcgtcgaa acattcggcg atgacatggc acccggcgaa
    15361 ggtctcgggg atgttgcgcg cggtcagccg cagattgcgc gtttcgatcc gcacatacgg
    15421 attcatccgc agcaggttgt cgcgcagggc gtccactttg ggcaggccga tctgatcgat
    15481 gaaaaactgc tggcgattca gattgctcgg ttcgacgacg tcgaaatcga ccagcagcag
    15541 gtggccgacg ccggtccggg ccagcgcgat ggcgacggcg ctgcccaggc cgccgcagcc
    15601 ggcgatccca acgcgcgccg ccttgacgcg cgcatgcacg ccggggccgt ggcgcgcgac
    15661 catcaggctt tccaattcct cgggcgaagg gacttcgccg cgccggatca gaaccacctc
    15721 gtcgccagcg gccaaggggc ggtcctcggc cagcgggaac ccgttgagaa tcacgacgtc
    15781 ggcgtcgggc ttgagccggt cgcgcagttg aaacagcgtg acgccgggcg cgacgcggtg
    15841 cggacgctca ttgacgcgca gcggcagccc ttccgcgtcg gcgcgcggcc cctccgtgcc
    15901 gatctccagg ggaaattgaa cgggcggttt cacgttgcgg gttccctacg ccggcattac
    15961 ccggatcagg ttcatcgggt tcgcggacta ccgctgtctc agccccttga gcggagcacc
    16021 ccatgccgtc ccatgttacg ctcccgcgag ccgggccgtc aatctccccg cccctcactt
    16081 tagaatcgtc cctcgtctct cgtctcgtct ctcgtccctc gtcctcgtga tcgccctcaa
    16141 aggacctctc caagattcgg ctctcggctc tcgtcctcgt cctcggctct cgaaaaccag
    16201 ccttcccatt ccccctctcc c
//
LOCUS       DAOPCO010000058        16177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3667_length_16177_cov_4.233284, whole genome shotgun sequence.
ACCESSION   DAOPCO010000058 DAOPCO010000000
VERSION     DAOPCO010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16177
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3667_length_16177_cov_4.233284"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLS90_06235"
     CDS             complement(<1..850)
                     /locus_tag="PLS90_06235"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPK02038.1"
                     /translation="MALAAPPSTSPITPAEQRERLAQLLASRSPAPRRILVIGPDITR
                     CYSGAGGLTSLLFELAAGRCHIDLMPALGTHEPMTPAELDRMYPGVPHDRVLVHDWRN
                     DLLRIGEIPAGRIEQLSAGRLHFSIAVEINRRLLEGGYDLILSVGQVVPHEVIGMANH
                     SKNLFIGVGGGELLNKSHYLGAVVGMEQIMGRMNNPVRSVMDDAAAHGLAGLPIVYVQ
                     TVIAPDPQGRPALHGLFIGEGRRLRGGRAPQPAPQHHRRRTPAQEVRRLPRTTRVPHH
                     LARQQVR"
     gene            complement(914..2365)
                     /gene="gnd"
                     /locus_tag="PLS90_06240"
     CDS             complement(914..2365)
                     /gene="gnd"
                     /locus_tag="PLS90_06240"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPK02039.1"
                     /translation="MSQADIAVVGLAVMGENLALNMESKGFTVAVFNRTVEKVDQFLA
                     GRAKGKNFVGAHSLPELAAQLKRPRKIMLMVKAGRPVDDFIEALIPHLEPGDMIIDGG
                     NSHFPDTRRRTKYLEEKGLLYIGTGVSGGEEGALKGPSIMPGGSTQAWPHVKDIFQKI
                     AAKVDDGSPCCDWVGEDGAGHFVKMVHNGIEYGDMQMICEAYHLMRVGLGLSNDEMHE
                     VFAEWNRGELDSYLIEITRDILAYRDEHGDAVVDKILDTAGQKGTGKWTAIEALNLGQ
                     PLTLIGEAVFSRCLSALKDERVAASKQLSGRTQAPRLDRDQAIDDLRQALYASKIVSY
                     AQGYQLMRAAAAEYGWNLNYGGVALMWRGGCIIRSAFLGKIKEAFDAQPGLTNLLLDP
                     FFKQATDAAQPAWRRTLTRAIELGIPMPAMTSALAFFDGYRSAWLPANLLQAQRDYFG
                     AHTYERTDRPRGQYFHTNWTGEGGDTASTAYVV"
     gene            complement(2456..3343)
                     /locus_tag="PLS90_06245"
     CDS             complement(2456..3343)
                     /locus_tag="PLS90_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK02040.1"
                     /translation="MPAPEPLQTFQPRHPFFVGIDSDGCVFDSMELKQKECFIPNIVR
                     VWGLQGISKYARETAEFVNLYSRQRGVNRWIALVQVFDLLAERPEVRARQVALPDDAP
                     LRDWLTSGRPLTDKTLEALIAENPHPTLLTAREWSRAVNESVTATVANLPPFPYVRES
                     LERLRAEADLMVVSQTPAAALEREWAEHDLTRFVCLIGGQELGTKGEILQHAAQGRYP
                     SSKILMIGDAPGDLKAARSAGALFFPINPGDEEHSWKRFYEEGIDAFLEGRFEGEYEA
                     GLLADFNRRLPESPPWKTA"
     gene            3671..4765
                     /locus_tag="PLS90_06250"
     CDS             3671..4765
                     /locus_tag="PLS90_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPK02041.1"
                     /translation="MAINMHKLLEGMVRVDASDLHLKVGGPPMIRMNGQLHPIDHPPL
                     EAEDTEAANTLMMPPRCREQLERDGTVDYSYGLSPTQRFRINCFHQRGTKSLAIRKLV
                     SKKVTLEDLNLPPQLTRLAKYHRGLVLVTGVTGSGKTSTLAAIINIINATRREHIITI
                     EDPIEIVYTDEKCIIDQIEVNQDVVDFKCALRHALRQDPDIILLGELRDRETIETAMH
                     CVDTGHLVFSTLHTPDARQTITRITHYFTLEEMPLILDQLSKNLQAVVCQRLVRTADG
                     KGRVPCCEIMFNNPIIQKLIIEERIDDIQQVMRSGAEGMQTFDMHLVQLVKSERVTME
                     EANTVVEDEAAFRRLMKGISAGGDRGGLIG"
     gene            4806..5498
                     /locus_tag="PLS90_06255"
     CDS             4806..5498
                     /locus_tag="PLS90_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02042.1"
                     /translation="MALIECRGLEKIYHMGEQVVHALDGVDLAIEDGEMLAIIGSSGS
                     GKSTLMNILGCLDRPTAGSYMLDQHDVSRLPDDELARVRNRKIGFVFQQFNLLARSTA
                     LENVEVPLFYRGDAHPGDLARRSLERVGLANRMDHYPNQLSGGQRQRVAIARALVTEP
                     SIILADEPTGNLDSVTTEEIMQLFLELHRQGVTLIIVTHEPNIAEYCERQIRIRDGKI
                     VEDQRHGAPAKG"
     gene            5503..6489
                     /locus_tag="PLS90_06260"
     CDS             5503..6489
                     /locus_tag="PLS90_06260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPK02043.1"
                     /translation="MASEAATLETRPDLRVRIGPLELKNPVTVASGTYGYGTEYAEFH
                     DPGRLGAIFLKGLTLEPRGGNAPQRLVETPSGLMNAIGLQNVGLEEFIRYKLPAIEGL
                     ATLCIANICGSTMEEYVTLAERLSAIARVDAIELNISCPNVKSGCIAFGSDPRMTEEV
                     TRRVVRATDRPVIVKLSPNVTSIAEMARAAEAGGAAGLAVVNTFLALAIDVKTRRPKL
                     GNVTGGLSGPAIRPIAVRMVWDAYQAVRIPIIGQGGITCARDALEFFIAGASAISIGT
                     QNFVDPLAPITVLEGIEQYLIENGMARLSELVGSLQIPEEAPAAIRTGGARE"
     gene            6668..8281
                     /locus_tag="PLS90_06265"
     CDS             6668..8281
                     /locus_tag="PLS90_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02044.1"
                     /translation="MPETEEVTRHPQPIVLVRGYGGLRVEDEKRNAYQGFNDGTVYPH
                     RRGENYIYEGLILRFLKSEWRFQDATNVVNYYGRVREEAVELPEDLQGLDAGCFSGHK
                     IVIDPAMALRLIRQPEDPCRTLWVFRYYDLDDREFRVYAEALVRLIQFIRALVRLKTG
                     HETKVNIVAHSMGGLIVREAVQMVFCQGEATRAINKIATLGTPHRGISFQVVKRWIAI
                     DGADELERFNPDFQENRKNQESYLNFHKHFPVERLLTVVGTNYQAYGTRPASWANRWF
                     GAEGEGGPTYNRSDGLVKQISAQIPGAPRTFVHKCHGGEDSLVTSREAFEIITRFFFG
                     NIRVRLRLVDALIKRGRDAFGRSEYFFGVSVKPRRVDFELFHQSKEAENCYGPFRERD
                     LSDAGVAFAWAGGGRLIWEGWLDTRAIERDPENKEKDLVVRLECYVSERDTRGIGFSD
                     NVIFSGQVYVRALLPTARRGLSLALHTSEDFSQSQFKKKMKPVEEGWQFSVGDRSFEG
                     TLQLELERVPEYGPPEPLVWRAGEGKDQP"
     gene            complement(8340..9398)
                     /locus_tag="PLS90_06270"
     CDS             complement(8340..9398)
                     /locus_tag="PLS90_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HPK02045.1"
                     /translation="MSPTPTSPEWLRFLFRPVLVARVFFVLLTSWAGMLLADKGSLVE
                     FLGVTFNQQLFYALIGFLAAVVIIIVEYATDAISSHKILMAAMGATAGLVFASLAYPI
                     IPASVASPHTARMICSLMFGYFGIVLAIKHAERFHLSRLQFLLSPSAEAPKVLDSSVI
                     IDGRIRELIELQVIKGAVLVPTFVLNEIQAIADSNDPNRKARGRRGLEILESLREICR
                     TLELADKDYPDLTEVDNKLVQFCREMQADLITNDYNLQKIAQLHKITVININEIANAL
                     RPTVYIGENFLLTVVREGKEPGQGVGYLEDGTMVVVDEAVNFLGQEIEVSVSSILQTS
                     SGRLVFARPREETRRLRA"
     gene            complement(9531..10871)
                     /locus_tag="PLS90_06275"
     CDS             complement(9531..10871)
                     /locus_tag="PLS90_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02046.1"
                     /translation="MCNARLSRPLGLALFLFTLSLAAPAQPLFPPGVALERFDERDDP
                     PPPFGYDAEFIRIDWTHPLLAADVVNHLAGGAAPPAWAEADRKASGRLRIQLAAPLSA
                     GAGPERWRPALPLIAPADLRAADGVNDTSRRVAALAEPFLLLAGARLWLPVAPGAPEL
                     APPPLGPREQTPALVGLRWLGTASTLDRLPDLPLDGRALLSLTSPLTGLTRFVVVAHR
                     DGSSGETGDLVDWLRLQADGGDWIALHDVGPGCFIGQGDWRLTAPGNPRRVERLGITL
                     ELRPHQPGELVDWTRMAGCTVRASSSERDFDPARATRGHLLPFPFSPPVWISRAETNE
                     PEPPWFEVRLGDPRPVERIELFWPSAVGFSGQFNPGRVRVHLQGPRGLAAEEELVLDN
                     PEGPHTLLAWDEPRQISAVTLIFDQPSRMPLDRRARLAGLRLWGPWDGSTPALP"
     gene            complement(10937..12220)
                     /gene="purD"
                     /locus_tag="PLS90_06280"
     CDS             complement(10937..12220)
                     /gene="purD"
                     /locus_tag="PLS90_06280"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPK02047.1"
                     /translation="MRILIVGGGGREHALAWKIARSPLCDKLFCAPGNPGIGRVAQCV
                     PIPLGPPFHELTAWCREQAIDLVVIGPERPLADGIVDALAGAGIKAFGPTRDGAQLEA
                     SKTFAKDLMREAGIPTAQARTFSNLDAALAALDDGPPPYVIKAVGLAEGKGVTVARTR
                     AEAEDALRRCLCDRVFGQAGAEVLIEEFLAGEEASLLAFTDGRVIQAMDSAQDHKPLL
                     DGDQGPNTGGMGAYSPAPVVTPAIFRQCVAHVLEPCLAALRRRGIDYRGVIYAGLMIT
                     ADGPRVVEFNCRFGDPETQALLPRLQNDLVEVLLACVEGRLAGIQLRWRDDAAVCVVA
                     ASGGYPGPYPRGKAISGLDSVAEDDRTVVFHAGTALDASGRVVTAGGRVLGITACDDS
                     LRAAIDRCYAVARQIHFDGMHYRTDIGRKALDRLD"
     gene            complement(12217..14349)
                     /locus_tag="PLS90_06285"
     CDS             complement(12217..14349)
                     /locus_tag="PLS90_06285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK02048.1"
                     /translation="MPPQSALPARSFPLLRLFALGLFTLAAAVLPAAPPYAVSGRAPQ
                     LAETVVARTPHGDVTALDLLIFDDLNDRLIQTLPASSLLDLEHAPPPHVRTVNELVQR
                     YAITERLADQAGAWQPAPDLLRPAAYGAAEAIWVEHVIKPEVTVDPTDVDRYYLSHPE
                     RYLQRRQAQVRYIFRTVPDMSQALAREAVRDELEALRARIEKGELTFDEAARQFSDAP
                     SAAEGGLLPAFFDNTYFAEFEEESFKLSEPGRMSPVFSGPGGFYLVQLVHQQRPRNIP
                     IEAVRDQIAEQLQYDLIASYYNFELARIQRRRYVRNHAPWFEYMNFSAPLARVDRATL
                     SRQQFFRFYGNPIDPFFLVNWPAVVGGIGHWVEGETVMQELEDAGLANHPWIARARQI
                     ATIERKAQHVIAVSVPPATYSSGDVALETLKQNPEFLASLRSLYLVRISIAPRDPAGM
                     TPTELRFAGNTVHQISQSLAQGILPTEPQSINLADWRASLMQTLADQGLTLDSTPPDA
                     PEVLVVTGGATPIEFTIPAPPTPVRFALAELENAIAATRWTEVDVAVEPFGWRDTVPG
                     SGWDRLFLYVQPGQVSQPTRLGSITERYLVLDERPLDVTPWLERPLHLQSLAFQAGAH
                     DIIAATQRELLQSGSVQLAWSPTPAAEPPADTATLPDNPPPAPDFERIELPPPPPADA
                     VQPPQDLAPFDIVIDDPEQTDTLESPPE"
     gene            14776..>16177
                     /locus_tag="PLS90_06290"
     CDS             14776..>16177
                     /locus_tag="PLS90_06290"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPK02049.1"
                     /translation="MRSDTIKKGHERAPHRSLLRATGVREDDWNKPFIAICNSYVEII
                     PGHVHLNAVGQFVRDAVRAAGGVPFVFNVIGVDDGIAMGHSGMKYSLPSRELIADSLE
                     TMVRAHCFDGMICIPNCDKIVPGMMMGAMRCNIPTVFVSGGPMEAGEGPGGRQLDLID
                     AFYAVGQREVGQISEDDFARVEQNACPTCGSCSGMFTANSMNCLNEALGIALPGNGTI
                     LATSGERLRLYQRAAETIMKLVERDIKPRDIATPAAFDNAMVLDMAMGGSTNTLLHVL
                     AVAREAGVEYDMGRINELSQKTPNICKVAPSATPEGRLYHIQDVHRAGGIQTILGALE
                     RGMPGLLDTSCLTVTGRTLGENLAAYDVRRETVSEDAIAMYVEGCIPSGKSVEQVRSE
                     LFAAGGRAPQRRTAAGGNGGVAVAANPCVEATATFDPHDVIREVKRAYSPEGGLTILY
                     GNLAREGCVVKTAGVDP"
BASE COUNT         2772 a         4380 c         6385 g         2640 t
ORIGIN      
        1 gacggacttg ttgccgagcc aggtggtgcg gaactcgtgt ggttcgaggt agacgacgca
       61 cttcttgagc gggcgttcga cgacggtgat gttgaggcgc tggctgaggc gcgcggcctc
      121 ctcgaaggcg gcggccctcg ccgataaaca ggccgtgcag ggcggggcgg ccctgcgggt
      181 cgggcgcgat gacggtctgc acgtagacga tcggcaggcc ggcgaggccg tgggcggcgg
      241 cgtcgtccat caccgagcgg acggggttgt tcatccggcc catgatctgt tccatgccga
      301 cgacggcgcc caggtaatgg ctcttgttga gcagctcgcc gccgccgacg ccgatgaaga
      361 ggttcttgct gtggttggcc atgccgatca cttcgtgcgg cacgacctgg ccgacggaga
      421 ggatcaggtc gtagccgcct tcgagcaggc ggcggttgat ctcgacggcg atggagaagt
      481 ggaggcggcc ggcggagagc tgctcgatgc ggccggcggg gatctcgccg atgcgaagga
      541 ggtcgttgcg ccagtcgtgg acgaggacgc ggtcgtgggg gacgccgggg tacatccggt
      601 cgagctcggc gggggtcatc ggctcgtggg tgccgagggc gggcatcagg tcgatgtggc
      661 agcggccggc ggcgagctcg aagaggagcg aagtgaggcc gcccgcgccg gagtagcagc
      721 gggtgatgtc ggggccgatg acgaggatgc ggcgcggcgc gggggagcgg gaggcgagga
      781 gttgcgcgag gcgttcgcgt tgctcggccg gggtgatcgg ggaggtgctc ggcggtgcag
      841 ccagggccat gatggtgatt gcgtctccgg taaaggggct agcgggggag cggaggaagg
      901 gatcgccggc gggtcaaacg acatacgccg ttgaggccgt gtcgccgcct tcgccggtcc
      961 agttggtgtg gaagtactgg ccgcgggggc gatcggtgcg ttcgtaggtg tgggcgccga
     1021 agtaatcgcg ctgggcctgg agcaggttgg cggggagcca tgcgctgcgg tagccgtcga
     1081 agaaggccag cgcgctggtc atggcgggca tcgggatgcc gagttcgatg gcgcgggtca
     1141 gcgtgcggcg ccaggcgggc tgggcggcgt cggtggcctg tttgaagaag ggatcgagca
     1201 gcaggttggt caggccgggc tgggcgtcga aggcttcctt gatcttgccg aggaaggcgg
     1261 agcggatgat gcagccgccg cgccacatga gcgccacgcc gccgtagttg aggttccagc
     1321 cgtattcggc ggcggcggcg cgcatgagct gatagccctg ggcgtagctg acgatcttgg
     1381 aggcatagag ggcctggcgc aggtcgtcga tcgcctggtc gcggtcgagc cggggcgcct
     1441 gcgtccggcc agagagctgt ttggacgcgg cgacgcgctc atccttgagc gccgagaggc
     1501 agcgcgagaa gacggcctcg ccgatgagcg tgagcggctg gccgagattg agcgcctcga
     1561 tggcggtcca cttgccggtg cccttctggc cggcggtatc gaggatcttg tcgacgacgg
     1621 cgtcgccgtg ctcgtcgcgg taggcgagga tgtcgcgggt gatttcgatc aggtaggagt
     1681 cgagctcgcc gcggttccac tcggcgaaga cctcgtgcat ttcgtcgttg gagaggccga
     1741 ggccgacgcg catgaggtgg taggcttcgc agatcatctg catgtcgccg tattcgatgc
     1801 cgttatggac cattttgacg aagtggccgg cgccgtcctc gccgacccag tcgcagcagg
     1861 gggagccgtc gtcgaccttg gcggcgatct tctgaaagat gtccttgacg tggggccagg
     1921 cctgggtgga gccgccgggc atgatggagg ggcccttgag ggcgccctcc tcgccgccgg
     1981 agacgccggt gccgatgtag agcaggccct tttcctcaag atatttcgtg cggcggcggg
     2041 tgtcggggaa gtgggagttg ccgccgtcga tgatcatgtc gcccggctcg aggtggggga
     2101 taagggcctc gatgaagtcg tcgacgggcc ggccggcctt gaccatgagc atgatcttgc
     2161 gggggcgttt gagctgggcg gccagttcgg ggagcgagtg ggcgccgacg aagttcttgc
     2221 ccttggcgcg gccggccagg aactggtcga ctttttcgac ggtgcggttg aagaccgcca
     2281 cggtaaagcc tttgctttcc atattaagcg cgaggttttc gcccatcacg gcgagtccga
     2341 caacggcaat atccgcctgc gacatggttg ctatccttgt agatgaatct tcattgaata
     2401 cggggttggg ttcgattgcg ataccgatac cgatagtgat accgataccg ctttttcatg
     2461 cagttttcca cggtggggat tcggggaggc ggcggttgaa gtccgcgagc aacccggcct
     2521 cgtattctcc ctcgaaccgc ccctcaagga aagcgtcgat tccttcctcg taaaaccgtt
     2581 tccatgaatg ttcttcgtcg ccggggttga tcgggaagaa gagggcgccg gccgagcggg
     2641 cggccttcag gtcgccgggt gcgtcgccga tcatcaggat tttggaggaa gggtaacggc
     2701 cctgggcggc gtgctggagg atctcgccct tggtgcccag ctcctggccg ccgatgaggc
     2761 agacgaagcg ggtcagatca tgttcggccc attcgcgctc gagggcggcg gcgggggtct
     2821 ggctgacgac catcaggtcg gcctcggcgc gcaaccgttc gagggattcg cgcacatagg
     2881 ggaagggggg gaggttggcg acggtggcgg tgaccgactc gttgacggcg cggctccact
     2941 cgcgcgcggt gaggagggtc gggtgaggat tttcggcgat gagggcctcg agcgtcttgt
     3001 cggtgagggg gcggccgctc gtgagccagt cgcgcagcgg ggcgtcgtcg gggagggcga
     3061 cctggcgggc gcggacctcg gggcgttcgg cgaggaggtc gaagacctgg acgagggcga
     3121 tccagcggtt aacgccgcgc tggcgggagt agaggttgac gaactcggcg gtttcgcggg
     3181 cgtatttgga gatgccctgc aagccccaga cgcggacgat gttggggata aagcattcct
     3241 tctgtttgag ttccatggaa tcgaagacgc agccgtcgga gtcgatgccg acgaagaagg
     3301 ggtggcgcgg ctggaacgtc tggagcggtt cgggggcggg cattcgatcg gttacctttc
     3361 gtaacaacgc gtggaactac ggaagtctga catagctata gggaagggga gaggaaaaag
     3421 aaaggaaaat tcggcggcgg gggggagggg ggattgtcgg acgtgagcga aaacaggcag
     3481 gggagggaaa ttcgagggac gagaacgagg gacgaatttt cgagcgggct tgacaggtat
     3541 gatacatctt ggaataagag actgttcgcg tccctggggg cgggatgcgg gcgatgacgc
     3601 aacgtccccg cgcgccagtg accctccgat cccgaattga gaccgcgaca gagaggttgt
     3661 aagactcccg atggccatca acatgcacaa actgctggag ggcatggtac gcgtcgatgc
     3721 ctccgatctg cacctgaagg tcggcggccc gccgatgatt cggatgaacg gccagttgca
     3781 tccgatcgac catccgccgc tggaagccga ggatacggaa gcggccaata cgctgatgat
     3841 gccgccgcgc tgccgggaac agctcgagcg cgacggcacg gtggactatt cctacggatt
     3901 gagcccgacg cagcgcttcc ggatcaactg tttccaccag cgggggacga agtcgctggc
     3961 gatccggaag ctggtgtcga agaaggtgac gctggaggat ctgaacctgc cgccgcagct
     4021 cacgcgtctg gcgaagtatc accgcgggct ggtgctggtg acgggggtga cggggagcgg
     4081 caagacgtcg acgctggcgg cgatcatcaa catcatcaac gccacgcggc gcgagcacat
     4141 catcacgatc gaggacccga ttgagatcgt ttataccgac gagaagtgca tcatcgatca
     4201 gatcgaggtg aaccaggacg tcgtcgattt caagtgcgcg ctgcgccacg cgctgcgcca
     4261 ggatccggac atcatcctgc tgggggagct gcgcgaccgc gagacgatcg agacggcgat
     4321 gcactgcgtc gacacgggtc acctggtatt ttcgacgctg cacacgcccg acgcgcggca
     4381 gacgatcacg cgcatcacgc actacttcac gctggaggag atgccgctga tcctggacca
     4441 gctttcaaag aacctgcagg cggtggtctg ccagcggctg gtgcggacgg cggacggcaa
     4501 gggccgcgtg ccgtgctgcg agatcatgtt caacaacccg atcattcaga agttgatcat
     4561 cgaggagcgg atcgacgaca tccagcaggt gatgcggagc ggggccgagg ggatgcagac
     4621 gttcgacatg cacctggtgc agttggtcaa gagcgaacgg gtgacgatgg aagaggccaa
     4681 tacggtcgtg gaggacgagg cggcgttccg gcggctgatg aaggggattt cggccggcgg
     4741 cgaccggggc gggctgatcg gctgacgcgg caggggcgag ggatccgggc ggcgaagagg
     4801 cgagcatggc gctgatagaa tgccggggac tggaaaagat ctaccacatg ggcgagcagg
     4861 tcgtgcatgc cctggacggg gtggatctgg caattgaaga tggcgagatg ctcgcgatca
     4921 tcgggtcctc gggcagcggc aagtcgacgt tgatgaacat cctgggatgc ctggatcgtc
     4981 ctacggcggg cagttacatg ctggatcagc acgacgtgtc gcgtctgccg gacgatgagc
     5041 tggcgcgcgt gcgcaatcgg aagatcggtt tcgtctttca gcagttcaac ctgctggcgc
     5101 ggtcgacggc gctggagaac gtcgaggtgc ccttgtttta ccggggcgac gcgcatcccg
     5161 gcgacctggc gcggcggtcg ctggagcggg tggggttggc gaatcgaatg gatcactacc
     5221 ccaaccagct ctcggggggt cagcgccagc gcgtggcgat cgcccgggcg ctggtgacgg
     5281 agccgtcgat cattctggcg gacgaaccga cgggcaacct ggattcggtg acgaccgagg
     5341 agatcatgca gctgtttctg gagctgcatc gccagggggt gacgctgatc atcgtgacgc
     5401 acgagccgaa catcgcggag tattgcgagc ggcagatccg gatccgcgac ggcaagatcg
     5461 tcgaagatca gcgccacggc gcgcctgcga aaggctgagg cgatggcatc cgaagccgcg
     5521 acgctggaga cccggccgga cctgcgggtg cggatcggcc cgctggagct gaagaatccg
     5581 gtgacggtgg cgtcggggac gtatggctac gggacggagt atgcggagtt tcacgatccg
     5641 gggcggctgg gggcgatttt tctgaaaggg ctgacgctgg agccgcgcgg gggcaacgcg
     5701 ccgcagcggc tggtggagac gccgtcgggg ctgatgaacg cgatcgggct gcagaacgtc
     5761 gggctggagg agttcatccg atacaagctg ccggcgatcg aggggctggc gacgctgtgc
     5821 atcgccaaca tctgcggcag cacgatggag gagtacgtga cgctggccga gcggttgagc
     5881 gcgatcgcgc gggtggatgc gatcgagctc aacatttcgt gccccaacgt caagtcgggc
     5941 tgcatcgcgt tcggttcgga tccgcggatg accgaggagg tgacgcggcg cgtcgtgcgg
     6001 gcgacggacc gaccggtgat cgtgaagctg agcccgaatg tgacgtcgat cgcggagatg
     6061 gcgcgggcgg cggaggcggg gggcgcggcg gggctcgcgg tcgtcaatac atttctggcg
     6121 ctggcgatcg atgtgaagac gcggcggccg aagctgggga acgtgaccgg agggctttcc
     6181 ggtccggcga tccggccgat cgccgtgcgg atggtgtggg acgcgtacca ggcggtgcgg
     6241 atcccgatta tcgggcaggg ggggatcacc tgcgcgcgcg atgcgctgga gtttttcatc
     6301 gcgggggcgt cggcgatctc gatcggcacg cagaatttcg tcgatccgct ggcgccgatc
     6361 acggtactgg aggggatcga gcaatatctg atcgagaacg gcatggcccg gttgtcggag
     6421 cttgtcggct cgttgcagat tcccgaggag gcccccgcgg cgatccggac gggcggcgcg
     6481 cgggagtaag ggtcgggccg ccggacgggg ggcgcgcggg cccgccggtc gggggggcgg
     6541 ttgtccgggt tgcacggccg gttgagattt tcctgaagta ctttgccgta tcgttcgtag
     6601 taaggcggtc gccgatcggc cctgctgggc ggctgaactc ggtttgaacc ggaaggaggc
     6661 gccggccatg cccgagactg aagaagtcac gcgccatccc cagccgatcg tgctcgtgcg
     6721 cggctacggc gggctgaggg tggaggacga gaagcgcaac gcttaccagg gtttcaacga
     6781 cgggacggtc tatcctcacc ggcgcggcga gaactacatc tatgaagggc tgatcctgcg
     6841 ctttctgaag tccgagtggc ggttccagga tgcgacgaat gtcgtcaact attatggccg
     6901 ggtgcgggag gaagcggtcg aattgcccga ggacttgcag ggactggacg cgggctgctt
     6961 cagcgggcac aagatcgtga tcgacccggc gatggcgctc cggctgatcc gccagccgga
     7021 ggatccatgc cgcacgctgt gggtgttccg ctactacgat ctcgacgacc gcgagtttcg
     7081 ggtctatgcg gaggcgctgg ttcggctgat ccagttcatc cgagcgctcg tgcggctgaa
     7141 gaccggccac gagacgaagg tgaacatcgt ggcgcactcg atgggcggac tgatcgtgcg
     7201 cgaggcggtg cagatggtat tctgccaggg ggaggcgacg cgggcgatca acaagatcgc
     7261 gacgctgggg acgccgcacc gcgggatctc gtttcaggtg gtcaagcgct ggatcgcgat
     7321 cgatggggcg gacgagctgg agcgattcaa tcccgatttt caggagaatc ggaaaaatca
     7381 ggaatcgtat ttgaattttc ataaacactt tccagtcgag cggctcttga cggtcgtcgg
     7441 gacgaattac caggcctatg gcacgcggcc ggcttcgtgg gcgaaccggt ggttcggcgc
     7501 cgagggggag gggggcccga cctacaatcg cagcgacggt ctggtcaagc agatcagcgc
     7561 gcagattccg ggcgcgccgc ggacgtttgt acacaagtgc cacggcggag aggattcgct
     7621 ggtgacctcg cgcgaggcgt tcgagatcat tacccggttc ttctttggga acatccgcgt
     7681 gcggctgcga ctggtcgatg ccctgatcaa gcggggtcgc gacgcgttcg gccgcagcga
     7741 gtatttcttc ggcgtcagcg tgaagccgcg gcgggtcgat tttgagctgt tccaccagag
     7801 caaggaggcg gagaactgct acggaccatt tcgtgaacgg gacctcagcg atgcgggggt
     7861 cgcgttcgcc tgggccggcg gggggcggtt gatctgggaa ggctggctgg acacgcgcgc
     7921 gatcgaacgc gacccggaga acaaagaaaa ggatctcgtc gtgcggctgg agtgctatgt
     7981 gagcgaacgc gatacgcgcg gcatcggctt ttccgacaat gtgattttca gcgggcaggt
     8041 ctacgtgcgc gcgctgctgc cgaccgcgcg tcgagggttg tcgctggcgc tgcatacctc
     8101 tgaggatttc agtcagagcc agttcaagaa gaagatgaag ccggtggagg agggctggca
     8161 gttttcggtc ggcgaccggt cattcgaggg cacgctgcaa ctggagctgg agcgggttcc
     8221 cgagtacggg ccgcccgagc cgctggtgtg gcgcgcgggc gagggcaagg atcaaccctg
     8281 aacgggagcg tgcgatttgc agagagtttc caggatgctg atgtagacga ctttgggggt
     8341 caggcgcgga ggcggcgggt ttcctcgcgc gggcgggcga agacgaggcg gccggaggag
     8401 gtctgcagaa tactgctgac ggagacctcg atctcctggc cgaggaagtt gaccgcctca
     8461 tcgacgacga ccatggtgcc gtcctcgagg tagccgacgc cctggcccgg ttccttgccc
     8521 tcgcggacga cggtcaggag gaagttttcg ccgatgtaga cggtgggccg gagcgcgttg
     8581 gcgatctcgt tgatgttgat gacggtgatt ttgtggagct gggcgatttt ctggaggttg
     8641 taatcgttgg tgatcaggtc ggcctgcatt tcgcggcaga actgcaccag tttgttgtcg
     8701 acctcggtca ggtcggggta gtccttgtcg gccagttcga gggtgcggca gatttcgcgc
     8761 aaggattcga ggatctcgag gccgcgccgg ccgcgcgcct tgcgattggg atcgttggag
     8821 tcggcgatcg cctggatttc gttgagcacg aacgtgggga ccaggacggc gcccttaatc
     8881 acctgcaact caatgagctc gcgaatgcgg ccgtcgatga tgacgctgga atcgaggacc
     8941 ttgggggcct cggcgctggg cgagagcagg aactgcaggc ggctcaggtg aaagcgttcg
     9001 gcgtgcttga tggcgaggac gatgccgaag tagccgaaca tgaggctgca gatcatgcgg
     9061 gcggtgtggg gactggcgac gctggccggg atgatcgggt aggcgagcga ggcgaagacg
     9121 aggccggcgg tcgcccccat ggcggccatc agaatcttgt gcgaactgat ggcgtcggtg
     9181 gcgtattcga cgatgatgat gacgacggcg gcgaggaagc cgatcagggc gtaaaagagc
     9241 tgctgattga aggtgacgcc gaggaattcg acgaggctgc ccttatcggc caggagcatg
     9301 ccggcccagg aggtcagcag cacgaagaag acgcgcgcga ccaggacggg tcggaagagg
     9361 aaccgcagcc actccggcga agtcggggtt gggctcatgc ggcatcctct ttccgggggg
     9421 cgcgtggcga gaccgggggg cggcggaccc ggtggctgag attcagcttg taagctactc
     9481 ccggggcggg ccgaaagcaa gtccggcggg gggggcgagg cgggcgggcg tcagggcagg
     9541 gcgggggtgg agccgtccca ggggccccag aggcggaggc cggccagccg ggcgcggcgg
     9601 tcgagcggca tgcgcgaggg ctggtcgaag atcagcgtca cggcggagat ctggcgcggc
     9661 tcgtcccacg ccagcagggt atgagggccc tcggggttgt ccaggacgag ctcttcttcc
     9721 gcggcgaggc cgcgtgggcc ctggaggtga acgcggacgc ggccggggtt aaactggccg
     9781 gagaaaccga cggccgaggg ccagaacagt tcgatgcgct cgacgggccg gggatcgccg
     9841 aggcgcactt cgaaccacgg cggttccggt tcattcgtct cggcgcggct gatccagacg
     9901 ggcggggaaa aggggaaggg gaggaggtgg ccgcgggttg cgcgggcggg gtcgaaatcg
     9961 cgctcggatg aggaggcgcg cacggtgcag ccggccatgc gggtccagtc aaccagctcg
    10021 ccgggctggt gcgggcgcag ttccagcgtg atgcccaggc gttcgacgcg gcgcggatta
    10081 ccgggcgcgg tcagccgcca gtcgccctgg ccgatgaaac agccgggccc gacgtcgtgc
    10141 agggcaatcc agtcgccgcc gtccgcttgc aggcgcagcc agtcgacgag gtcgcccgtt
    10201 tcgcccgagg agccgtcgcg atgggccacg acgacgaacc gggtcaggcc ggtgaggggc
    10261 gaggtcaggc tgagcagcgc ccggccgtcc agggggaggt caggcagccg atcgagcgtg
    10321 gaggccgtgc ccagccagcg caggccgacg agggcggggg tttgctcgcg cgggccgagg
    10381 ggcggcgggg caagctcggg cgcgccgggg gcgacgggca accagaggcg cgccccggcc
    10441 aggaggagga agggttcggc gagagcggcg acgcggcgcg aggtgtcgtt gacgccgtcg
    10501 gcggcgcgca ggtcggcggg ggcgatcagc ggcagcgcgg gccgccaacg ctcggggccg
    10561 gcgccggcgg atagcggcgc ggcaagctgg atccgcagac ggccggaggc tttccggtcc
    10621 gcttcggccc atgccggggg cgcggcgccg ccggcgaggt gattgaccac gtcggccgcg
    10681 aggagcgggt gcgtccagtc aatgcggatg aattcggcgt catagccgaa gggaggcggc
    10741 gggtcatcgc gctcgtcgaa acgctccaag gcgacgcccg gggggaagag gggttgagcg
    10801 ggcgccgcga gggagagcgt gaacaggaac agcgcaaggc cgaggggacg tgagagacgc
    10861 gcgttacaca tgaaccggcc cagcgtggga tcaagctcga cggaggatgc gaggcgcgga
    10921 cgcggggcaa gaaggatcag tcgaggcggt cgagcgcctt ccggccgata tcggtgcgat
    10981 agtgcatccc gtcgaagtgg atctggcgcg cgacggcgta gcagcggtcg atcgccgcgc
    11041 gcagcgagtc atcgcatgcg gtgatgccca ggacgcggcc gccggccgtg acgacccggc
    11101 cgctcgcatc gagcgccgtc cccgcgtgga aaacgaccgt ccggtcatcc tcggcgacgg
    11161 aatccagccc cgagatcgct tttcccctgg ggtaggggcc tgggtagccg cccgaagcgg
    11221 cgacgacgca gacggcggca tcgtcgcgcc agcgaagctg gatgccggcg agccgccctt
    11281 cgacgcaggc cagcagcacc tcgaccagat cgttctggag ccgcggcagc agggcctggg
    11341 tctcgggatc gccaaagcgg cagttgaatt cgaccacacg ggggccgtcg gcggtaatca
    11401 tcaggccggc atagatcacg ccgcgatagt cgatgccccg gcggcgcagg gcagcgaggc
    11461 agggttcgag gacgtgcgcg acgcactggc ggaagatcgc gggggtgacg accggggcgg
    11521 gcgaataggc gcccatgccg ccggtgttgg gcccctggtc gccatcgagc aggggcttgt
    11581 gatcctgcgc gctatccatg gcctggatga cgcggccgtc ggtgaaggcg agcaggctgg
    11641 cctcttcgcc ggcgagaaac tcctcgatca ggacctcggc gccggcctgg ccgaagacgc
    11701 gatcgcacag gcagcggcgg agggcgtcct cggcctcggc gcgggtgcgg gcgacggtaa
    11761 cgcccttgcc ttcggccaga ccgacggcct tgatgacgta ggggggaggc ccatcgtcca
    11821 aggccgcgag ggcggcgtcg aggttggaga aagttcgcgc ctgggccgtg gggattccgg
    11881 cttcgcgcat caggtccttg gcgaaggtct tggaggcttc gagctgggcg ccgtcgcgcg
    11941 tggggccgaa ggccttgatg cccgcaccgg ccagggcgtc gacgatgccg tcggcgagcg
    12001 gacgctccgg gccgatgaca accagatcaa tggcctgctc gcggcaccag gcggtcagtt
    12061 cgtggaaagg cgggccgagc gggatgggga cgcactgagc gacgcggccg atgccgggat
    12121 ttcccggggc gcagaataat ttgtcgcaca agggactgcg cgcgattttc caggcgaggg
    12181 cgtgctcacg gccgccgccg ccgacaatca gaattctcat tccggcgggc tttcaagggt
    12241 gtcggtctgt tcggggtcgt cgatgacgat gtcgaatggg gccagatcct gagggggctg
    12301 gacggcgtcg gcggggggcg gcggcggcaa ttcgatgcgc tcgaaatcgg gggcgggggg
    12361 tgggttatcg gggagcgtgg cggtgtcggc cggcggctcg gcggcgggcg tcggggacca
    12421 ggcgagctgg accgaaccgc tttggagcag ttcgcgctgc gtagccgcga tgatgtcgtg
    12481 cgcgcccgcc tggaacgcga gggactgcaa gtggaggggg cgctccagcc agggggttac
    12541 atccaagggt cgctcgtcaa ggaccagata gcgttcggtg atgctgccca ggcgggtggg
    12601 ctggctgacc tgaccgggct gaacatagag gaagaggcga tcccagccgg agccggggac
    12661 ggtatcgcgc cagccgaagg gttcgacggc aacgtcgacc tcggtccagc gggtggcggc
    12721 gatggcgttt tcgagttcgg ccagggcgaa gcgaacgggg gtcggcgggg cggggatcgt
    12781 aaattcaatg ggggtggcgc cgccggtgac gacgaggacc tcgggggcat cggggggcgt
    12841 ggagtcgagc gtcaagccct gatcggcgag ggtctgcatc aacgaggcgc gccagtcggc
    12901 gagattgatc gattggggct cggtggggag gattccctgg gcgaggctct ggctgatctg
    12961 gtggactgtg tttccggcga agcgcagttc ggtgggggtc atgcccgcgg ggtcgcgcgg
    13021 cgcgatgctg atccggacga ggtacaatga gcggaggctg gccaggaatt cggggttttg
    13081 tttgagagtc tcgagggcga catcgccgga gctgtaggtg gcggggggga cgctgacggc
    13141 gatgacgtgc tgggccttgc gctcgatcgt ggcgatctgg cgggcgcggg cgatccaggg
    13201 gtggttggcg aggccggcgt cctcgagttc ctgcatcacg gtttcgccct cgacccaatg
    13261 gccgatgccg ccgacgacgg caggccagtt gaccaggaag aaggggtcga tcgggttgcc
    13321 gtagaagcgg aagaactgct ggcgggagag cgtggcgcgg tcgacgcggg cgaggggggc
    13381 ggagaagttc atgtattcga accagggcgc gtgattgcgg acgtagcgcc tgcgctgaat
    13441 cctggccaat tcgaagttgt agtaggaagc gatcagatcg tattgaagct gttcggcgat
    13501 ctggtcgcgg accgcttcga tcgggatgtt tcgcggccgc tgctggtgga cgagttggac
    13561 cagatagaaa ccgcccgggc cgctgaaaac gggactcatc cggccgggtt cgctgagctt
    13621 gaaggattcc tcctcgaact cggcgaaata ggtattgtcg aagaatgccg ggaggagacc
    13681 gccctcggcg gccgaggggg cgtcggagaa ctggcgggcg gcttcgtcga aggtcagctc
    13741 gcccttttcg atccgggcgc gcagcgcctc cagctcgtcg cggactgctt cgcgggcgag
    13801 ggcctgactc atgtcgggga cggtgcggaa gatgtagcgg acctgggcct ggcggcgctg
    13861 gaggtagcgt tcgggatgcg agagataata gcggtcgacg tcggtgggat cgacggtgac
    13921 ctccggtttg atgacgtgct cgacccagat ggcctcggcg gcgccgtagg cggccggacg
    13981 gaggagatcg ggggcgggtt gccaggcgcc ggcctggtcc gccagtcgtt cggtgattgc
    14041 gtagcgctgc accagttcat tcacggttcg gacgtggggg gggggcgcat gttccaggtc
    14101 gagcaagctg gaggccggca gggtctggat cagccggtcg ttgaggtcgt cgaagatgag
    14161 cagatcgagc gcggtgacat caccatgggg cgtgcgggcg acgacggttt ccgccagttg
    14221 cggggcgcgg ccgctgaccg cgtagggggg cgcagccgga aggacggcgg cggccagcgt
    14281 gaagagcccc agcgcgaaca gacgaagcaa ggggaaggac cgggccggca aggcggattg
    14341 cggaggcatg caggtgttca ctccagcgga tcgaatcggc gggcgcaacc cgaagtaccc
    14401 gaaaggtttg acataccaga ggggtgaaat caagggatta gcggcgcgat gggggggtgg
    14461 gtgtggaggg cgccgccatc gtgctcgaac gaacctgaca ataacctgaa gcgccccagt
    14521 cgttgcagga aagccgcacg tcgacgttgg tggccggcgc gttgccgggg ttatggacgg
    14581 tgacggtgaa atgggtgtgc ctgctggggc aagctgaacc gggagattga ccgcaaggac
    14641 aggcaggagg tggacggggt ggactgggtg gaggaggtgg aagaggtgga agaggtggaa
    14701 ggctctggag aatgggtggt caattcagct agaatcaaga cagcacatca ctttgtccgg
    14761 gagaagccgc gatccatgcg ttccgacacg atcaaaaaag gccacgaacg ggcgccgcat
    14821 cgttcgctgc tgcgcgccac cggcgtgcgc gaagatgact ggaacaagcc gttcatcgcg
    14881 atctgcaaca gttatgtgga gatcatcccg gggcatgttc atctgaacgc cgtggggcag
    14941 ttcgtgcgcg atgcggtgcg cgcggccggg ggggtgccgt tcgtgttcaa tgtgatcggc
    15001 gtcgatgacg ggatcgcgat gggtcacagc gggatgaagt attcgctgcc gtcgcgcgaa
    15061 ctgatcgccg attcgctgga gacgatggtg cgggcgcact gtttcgacgg gatgatctgc
    15121 atccccaact gcgacaagat cgtgccgggg atgatgatgg gggcgatgcg ctgcaacatc
    15181 ccgacggtct ttgtgtcggg cgggccgatg gaggcggggg aaggcccggg cggccggcaa
    15241 ctggacttga tcgacgcgtt ctacgcggtc ggccagcgcg aggtggggca gatcagcgag
    15301 gacgatttcg cacgggtgga gcagaacgcc tgtccgacgt gcggctcgtg ctcggggatg
    15361 ttcacggcca attcgatgaa ctgcctgaac gaagcgctgg ggatcgcgct gccgggcaat
    15421 gggacgattc tggcgacgag cggggagcgg ttgcggctgt atcagcgcgc ggccgagacg
    15481 atcatgaagc tggtcgagcg cgacatcaag ccgcgcgaca tcgcgacgcc ggcggcgttc
    15541 gacaacgcga tggtgctcga catggcgatg gggggatcga ccaatacgct gctgcacgta
    15601 ctggcggtgg cgcgcgaggc gggcgtggag tacgacatgg ggcggatcaa cgagttgtcg
    15661 cagaagacgc ccaacatctg caaggtggcg ccgtcggcca cgccggaggg gcgcctctat
    15721 catatccagg atgttcatcg cgccggcggg atacagacga ttctgggggc gttggagcgc
    15781 gggatgccgg gtctgctcga tacgagttgc ctgaccgtga cggggaggac gctgggggag
    15841 aacctggccg cctacgatgt gcggcgggag acggtgagcg aggacgcgat cgcgatgtat
    15901 gtcgaggggt gcatcccgtc gggcaagagc gtggagcagg tgcggagcga gctgtttgcg
    15961 gcgggggggc gggcgccgca gcggcggacg gccgccggcg gcaacggcgg ggtggccgtc
    16021 gcggcgaatc cgtgcgtcga ggcgaccgcc acgtttgatc cccacgatgt gatccgggaa
    16081 gtgaagcgcg cctacagccc cgaagggggg ttgacaatcc tctacggcaa tctggcgcgg
    16141 gaggggtgcg tggtcaagac ggcaggggtg gaccccg
//
LOCUS       DAOPCO010000059        16081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3695_length_16081_cov_4.887183, whole genome shotgun sequence.
ACCESSION   DAOPCO010000059 DAOPCO010000000
VERSION     DAOPCO010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16081
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3695_length_16081_cov_4.887183"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLS90_06295"
     CDS             complement(<1..751)
                     /locus_tag="PLS90_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPK02050.1"
                     /translation="MAAAALGLVAGTAAAQPAAPAIDTGDTAWMLVSAALVLLMTPGL
                     ALFYGGLVRGKNVLGTMMQSLAAMALVGVLWVVCGYTIAFGSDLGHVVGAFDYLLLRG
                     VGGDPLDGQSIPHLLFVAFQGMFAIITPALIGGAVAERMKFKAFLLFMLLWVLLVYAP
                     LAHWVWGSGGWLLELGALDFAGGTVVHISSGFSALIACLMLGRRRGFPGEPILPHNLP
                     LVILGAGLLWFGWFGFNAGSALAANGTAALAL"
     gene            1564..2904
                     /gene="gdhA"
                     /locus_tag="PLS90_06300"
     CDS             1564..2904
                     /gene="gdhA"
                     /locus_tag="PLS90_06300"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPK02051.1"
                     /translation="MSYIQDVLNGLVRTHPLQPEFHQAVKEVLTSIAPVLDRYPHYRE
                     QAILERITEPERVILFRVPWMDARGRIQIHRGYRIEYNSALGPFKGGLRFHPTVNLSI
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKLDVEVMNFCQSFMTELQRHIGPNTD
                     VPAGDIGVGAREIGYLFGQYKRIRNEFTGVITGKRLNWGGSLIRPEATGYGTVYFAQN
                     MLAVRGEDLAGKVCTVSGAGNVAQYTVEKLIELGAKVVSLSDSNGTVHDPAGIDREKL
                     AWVMELKNVRRGRISEYANQFKGSIYREGLRPWSIPCDCAFPSATQNELSGDDARTLV
                     KNGCRLVAEGANMPTEPEGVDVFLANRVLFGPGKAANAGGVAVSGLEMAQNSEHTYWT
                     RETVDQRLRDIMKSVHEQAYNASVEYGATENYVVGANIAGFVKVADAMLDQGLV"
     gene            3655..6276
                     /locus_tag="PLS90_06305"
     CDS             3655..6276
                     /locus_tag="PLS90_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPK02052.1"
                     /translation="MAEDLRRERGYSTGLAELDALLGGLIPGDNIVWQVDQLADYEAF
                     ARPFAAGAVAVGHRTLYLRFADHAPVLADDAGVERVELDPQAGFEMFLGAIHATIGRA
                     GRGACYLFDSLSDLAVDWFSDQMLGNFFRLTCPYLYDMGTIAYFALLRDYHSLHATAP
                     IRETTQLFLDAYRHKGELYVRPRKVQQRHTPGMHLLHAWRGGRFVSVTDSHQAAEIMT
                     ASPRSTLDTAMDRLDVWNRAFLQAEEALRDQDRLGAADHDLDELHGRLLRMVISRDER
                     MLRLARRHLSLEEVLQIGRRTVGTGLIGGKSVGMLLARAILQRADPRWRDRLEVHDSF
                     YIASDVFYSFLVRNGCWWIRQNQRNPATFLQGVHIARRRILEGTFPEEVERQFAEILD
                     YFGPSPIIVRSSSLLEDNFGNAFAGKYESVFCPNQGTLEQRLENFLAAVRTIYASTMR
                     EEALNYRARHGILGRDEQMALLVQRVSGSLHDGYFYPQLAGVAYSFNPYAWSDQIDPR
                     AGLVRLVFGLGTRAVDRTDDDYTRIIALNSPERRPERSEEGVGRLAQQKVDALDLTSN
                     ALVTTDFSRILAQRPAIPMHLFASYDPDRGRYYRERGRSDGDPRALTFDRLIAETSFI
                     DDMREMLKTLQEAYAYPVDVEFTANFLDEREYKINIVQCRPLQVKGGMVAMALPDDVA
                     PEQTLLRTRGPVIGHSRVTPVDRVILVVPAAYGNLPAAERYAVARLIGRLNAVNDKET
                     TLLIGPGRWGTTTPALGITVSFAEIDRARVVCEIVAMREDLVPDVSLGTHFFSELVEM
                     EMLWLALFPTQSGNLFNQALLEAAPNRLTDLVPDAAHLAHVVRVVDAEDLAGRGQLRL
                     HADTLNQRVLCYLARGG"
     gene            6775..7686
                     /locus_tag="PLS90_06310"
     CDS             6775..7686
                     /locus_tag="PLS90_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK02053.1"
                     /translation="MEQRALGRTGDEVTMLAFGAAQLGQLMEDHQAAAALVDQLIDAG
                     IRFIDTAACYANSEEKLGPVMARRRGECFLASKCGHQVDPGDPPEWSPEIIRHSCERS
                     LRRLQTDHLDLLLLHTCSEEKLRDEAMIGALEQCKRDGLTRFIGYSGDNQSALTAIKS
                     GRFDCLEVTINFCDQSVLDEVLPAARAAGMGVLAKRTIANACWRELSRLPANHQAYIR
                     PYVDRLGKLGFTPQSLGYDGDWAELALRFTAFQPGVSTAIIGGTHFGHIQENIRAAAM
                     GPLPADVQARLREAWQASADASWIGLS"
     gene            complement(7844..8917)
                     /locus_tag="PLS90_06315"
     CDS             complement(7844..8917)
                     /locus_tag="PLS90_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02054.1"
                     /translation="MNRKLKIGFVGAGAMGQCAHLRNFASLPDCEVVALAELRPEMAR
                     QVAARHGVARVYPTHRELLAHEALDGIVAAQPFQRHGLLLPELLKAGVPLLTEAPLAS
                     SVPAGERIVEAVRASPAWLMVGYQRRCDPATQWVHGEIGRLRQTGELGPLRYLRVTMP
                     PGDWVANGFNGMMTSDETVRALEHEPPPADMDAPMYRAYLGFVNGYIHQVNLIRHLLG
                     EDWEIVSVDPTGVVLQGRSAGGLPCVLEMAPWTNSIDWQESALICFERGWLRLDLPAP
                     LAFNRAGSVELCYDPKGGTPERRRPQMPWEHAMRRQAIAFCQAIRGEIEPPCTAEEAL
                     KDLILARDYIRALQEAQAVTSGG"
     gene            complement(9639..12197)
                     /locus_tag="PLS90_06320"
     CDS             complement(9639..12197)
                     /locus_tag="PLS90_06320"
                     /inference="COORDINATES: protein motif:HMM:NF027456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK02055.1"
                     /translation="MDTTPLIAEPDLRIRKDDNSARVEAGGQLVSYEIFYINEGNQDA
                     TGVQVTETLPAFTTFNAGASTPGWMDQGGGVYTFDVGDLGVGPWEPPAPLVFAVNMLD
                     PLPSDYTVTTNTVTIADDGANGADPNPANNTASDITPLKTMSSSTLIVFEDLKVAQQL
                     YNDFEYNDFVMRSDVTETYDANNKLELIEMRVEALARGAIFNHIARLDLGLEGGVTAT
                     IQRFDANDVLVSQDIFVSADNPARSLQIFPLTKDALPPFPGGGGQLTNTEEQQSLAER
                     TEGHYALITIRPRNAPANERIVDDPATTLDEHNSAIYGYNLYVVNSGSTIYHGWDHNT
                     ATLDIVSGSQWPASPLIGFPLPQAMLLPTAFEYPVEYTPIWESHLDFEPWIKSGMTTN
                     QNWPLVNVVDEKTWPIDLAKALKRTAAAKAVDGLLFQQKLESAIPATPVLADMTQDGF
                     VDIVYGHYLNLIVIENAFNHSEVVIDPFPEILVDSQCSPVVVDLNGDGLLDVIRGYDN
                     GALVAYRHDGVELTPAGERLYLEGTIKGTPAVGDLDADGDPEIIVQTGHGLLYALGFD
                     FSIRPGFPVDLGGTRDIGSHHFMTPSPALAPLPAGDGLAIIAVNLSGKVFSITWAGDV
                     MPGWPVDLGGEVLASPAVGDLEGDGQIDIVIAMSDAPVYALTAEGQIKPGWPAQRRYG
                     GTSSPSLADVNGDGQLEVFVGSYDENLYGFSADGKTLPGFPVATQSQIFASPFFADLD
                     GDGTPEPLIGSSSGSLYAVCGESGPKADQPAVMAQATTLIATAGSVGDLDFDGRVEVV
                     FGSHDGRIRVVETEAPVVPAGKTWNGFRGGLRNPGMISSILQVGNAAQAWELYQ"
     gene            complement(12179..14788)
                     /locus_tag="PLS90_06325"
     CDS             complement(12179..14788)
                     /locus_tag="PLS90_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02056.1"
                     /translation="MKRKLLWATLLSLLAVTTAPAQISLNLGSIPAGKSVTITFDVAA
                     ADPLGAGVELISQQGTVTSGGVQSMTDDPDTVALDDPTTTVIVAAPDLELIKDDGATT
                     ATPGGTILYTLDYANNGDQDATGVEITDVVPEHTTYNAALSSPGWVLTGSGDAGSTLT
                     FAVGALPTTGTGTLNLAFDVNSPAPLGLTELINEARIDDDGSNGPDQNPADNITSDTT
                     PLDASPLLFLSKDDGGTTVTPGGTVIYTLFYTNAGDQDTTGVVITDLLPAHTSFDAGA
                     STPGWIEVNPGQFTFPIGALDVDTTGTVLFAVTVDNPVPSGVTSIDNSATITDQDANS
                     APAADSTPLIAMPDLAVSKDDGITTAVPGQTISYEIFYANNGDQASTGVVVTELLPPN
                     TSFNAAASTPGWVDQGGGVFTFDIGTLDGGGTNGTLTFAVDVDNPIDQYVEFIFNTVS
                     IADSGADGPDANPDDNGFDMQTPVAAFPDLSLLKDDGGTTVGAGGTVAYTLLWGNFGD
                     QDTTGVMILETLPEHTVFNPAASTPGWIDLGGGQFKFNVFDLDGGTTGTAIFAVDVVN
                     PVPVGVELIVNEAAIMDDGSQGPEFSLDDNMTSDTTPVIAGPDLSIDKDDGGVTVIPG
                     GTISYLLTYGNHGDQNTTGVVIMESVPADTVFNGAASSPGWVDQGGGVYRYEVGGLDV
                     GTTGTLIFAVDVNDPVGPFTEIITNITSPSDDGQGGPDLNPDDNSSTDTTPVDAEVEL
                     AISKTDGGVSVQPDGTVVYTIAYANLGNQDSPEVRITDTIPALTSYAAGSSRASWNRP
                     HTRSARVRWSRTTSACWAAIRPACCRSPSPSRTRCRRAPSRSRTSSSSMTFTPVSSPS
                     CRSTTRWRWTRRR"
     gene            complement(15102..>16081)
                     /locus_tag="PLS90_06330"
     CDS             complement(15102..>16081)
                     /locus_tag="PLS90_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02057.1"
                     /translation="KESYINLNKRFGISILNPTGTVDLLTVDGNRMSLNLVGVMSYVG
                     SGDHVLADAQITSNDLGSTFDTGIDFDFSGSALGNVAIVSNRAEGTKYFSRLTADQDA
                     QVAFEIRDNCPFGTVLTPFADGINVEMLADATANCRLNGTISGNRVTIPLQGFGEGIQ
                     VVQRGAGTMLVEILNNTINLDGGFLEGIRVETREGVNGNLQATIKNNTIKMTGTEPGC
                     GINMLGGGGAVTDRQTLCIDIAQNTVNDGTAGGAPDYQLVQRFGAAPGDNPFHLDGLG
                     ALSGTNAAVVEAFINGRNQGTPDTWVVTSLPRSDVNYTDADCDTPPVDP"
BASE COUNT         2759 a         5147 c         5470 g         2705 t
ORIGIN      
        1 cgagcgccag cgccgccgtc ccgttggccg ccagcgcgct gccggcgttg aagccgaacc
       61 agccgaacca caacagcccg gcccccagga ttaccagcgg caggttgtgc ggcaggatcg
      121 gctcgccggg gaacccccgc cgccgcccga gcatcaggca ggcgatcagc gcggaaaagc
      181 ccgaactgat gtgcacgacc gtcccgccgg caaagtcgag cgcgcccagc tccagcagcc
      241 agccgccgct cccccacacc caatgcgcca gcggcgcata gaccagcagc acccacaaca
      301 gcatgaacag caggaacgcc ttgaacttca tccgctcggc gacggccccg ccgatcagcg
      361 ccggcgtgat gatcgcgaac atcccctgaa acgcgacgaa tagcaggtgc gggatgctct
      421 ggccgtcgag cgggtcgccg ccgacgccgc gcagcagcag gtagtcgaac gcgccgacga
      481 catggcccag gtcgctgccg aaggcgatcg tgtagccgca gacgacccac aggacgccga
      541 ccagcgccat cgccgcgagg ctctgcatca tcgtgcccag gacgttcttg ccgcggacca
      601 gtccgccgta gaacagcgcc aggccggggg tcatcagcag caccagcgcc gcgctgacga
      661 gcatccaggc ggtgtcgccg gtatcgatgg cgggggcggc gggctgcgcg gcggccgtgc
      721 cggccactag ccccagcgcc gccgcggcca tcgggcggcg agtcgccgcg ggtatcgagc
      781 ggaccggtcg gaagcgacgc atgcctgata tcccccgtgg atcggattca ttttccacga
      841 cgctggagaa cggtgccaac atagggcctg cggacgctga ggttcaagcg aaaagaggaa
      901 cgcgaaagga gagatgggag aaccacgaac tacgaatccc gaaatccgaa acaaatttaa
      961 aaggtcaaat tcaaatgaag aacctttgga gtgaggcctt gaatcggtat cgggatcgct
     1021 aatcgccatc gatccccccc atcccgcagg gatgccaggc cctggccggt ggttgagcgc
     1081 agcgcccgca gggatgccag aacattgcag taacggcatc gggatcggta tcgatatcgc
     1141 tatccggatc gaccagaacg ggggtcgtaa accaccgctt cgaacggctg tgacctcgcc
     1201 ggaccccccc accgccagtc ccgtcaggga cggcagactt cagcccgcta cgtcagtggc
     1261 gggaatcgcc gaaaccagga aataagagtc ccgcagggac ggcagaaccg ctccaatcgg
     1321 ggtcccgcga cgcggcccca actttcccgc gtttcccccc cacattcgcc attgcatcga
     1381 tgggccccgc gctggtaaaa aaggatgttt ctgtcctctt ggcggcgccg ccgcgacagc
     1441 cgctgccgcc tcccgttccc tggacagatc gcatcccttc acgaagaccg tgctgcccgg
     1501 agcgggtgca ggggccagcc tccgccgccc ccgccctcgc gcagtccggc gggagagccg
     1561 cccatgagct atattcagga tgtcttgaac gggctggtcc ggacgcaccc gctccagcct
     1621 gaattccacc aggccgtcaa ggaagtcctc acctcgatcg cccccgtcct cgatcgctac
     1681 ccgcattacc gcgagcaggc gatcctggag cggatcaccg aacccgagcg cgtgatcctg
     1741 ttccgcgtcc cgtggatgga cgccaggggg cggattcaga ttcaccgcgg ctaccgcatc
     1801 gagtacaaca gcgcgctcgg gccgttcaag ggcggcctgc ggtttcatcc aaccgtcaat
     1861 ctgagcatcc tgaaattcct cgccttcgag caggtcttca agaatgctct caccacgctg
     1921 ccgatgggcg gcggcaaggg cggctccgat ttcgacccca agggcaagct cgacgtcgag
     1981 gtgatgaact tctgccagtc gttcatgacc gagctgcagc gccacatcgg ccccaacacc
     2041 gacgtcccgg ccggcgacat cggcgtcggc gcccgcgaga tcggctacct cttcggccaa
     2101 tacaaacgca tccgcaacga gttcaccggc gtcatcaccg gcaaacggct caactggggc
     2161 ggctcgctga tccgacccga ggcgaccggc tacggcacgg tctacttcgc ccagaacatg
     2221 ctggccgtcc gcggcgagga cctcgcgggc aaggtctgca ccgtctcggg cgccggcaac
     2281 gtcgcgcagt ataccgtcga aaaactgatc gaactggggg cgaaggtcgt ctcgctctcc
     2341 gattccaacg gcaccgtcca cgacccggcg ggcatcgacc gcgagaagct ggcctgggtg
     2401 atggagctga aaaacgtccg ccgcggccgg atcagcgaat acgccaacca gttcaaggga
     2461 tcgatctacc gcgagggact gcgcccctgg tcgatcccct gcgactgcgc gttccccagc
     2521 gcgacgcaga acgaactgtc gggcgacgac gcgcgcactc tggtcaagaa cggctgccgg
     2581 ctcgtcgccg agggggcgaa catgccgacc gagcccgagg gggtcgacgt cttcctggcc
     2641 aaccgggtcc tcttcgggcc gggcaaggcg gccaacgccg gcggcgtcgc ggtctccggg
     2701 ctcgagatgg cgcagaactc cgagcacacc tactggacgc gcgagaccgt cgaccagcgg
     2761 ctgcgcgaca tcatgaagag cgtccacgag caggcgtata acgcgtcggt cgaatacggc
     2821 gcgacggaaa actacgtcgt cggcgccaac atcgccggct tcgtcaaagt cgccgacgcc
     2881 atgctggacc agggcctggt ctgacccggc tcggcatcag gatcgggatc gctgtcggga
     2941 tcgctatcgg taccgcgatc ggtatcgcga tcgggatggg tatcgctatc gccatcggtt
     3001 ttgctgtcgg tctcggaatc agtccggccc ctcacccaaa tcctatttca actccgtccg
     3061 cgaaacgccg cgctcacgcg gagccgcaga gccacgggga gaagtacacc caactcgacg
     3121 cgagccgcca gcgccctatc tcctacggac gcgatgcaat gctctcaggc tctccccccc
     3181 gccggatggc cctaacccgg acaatacctt ttgccccccc gtcgacacga cccaactcga
     3241 caatccccaa ggcgcgcgca tgctcagctt gatgccgaca gaacggcgct ggcgtcggct
     3301 tgtcggcatt gcagacgtaa ccgcggagtt tccggcgtaa gattctttcc tgatataaca
     3361 agcagccacg ggatattatt gggcacggat ggcgggcggc ggaatcatcc gttgccctcc
     3421 gatgcctggc ggttcccagg ccgtggatgc catggccttc gggaggggga cgagggacga
     3481 tgttagcgtg agcttttcac gaccccttca gcgtcacctt gaaagggatg ataatgccct
     3541 gcatgcgtcc cttatgtccg ttatgtccat tatgtccgtt caccactttg cacgagaatc
     3601 agggacgacg agtctaaatc tgaggcgcga acgttcatgc accgggggat gaggatggcg
     3661 gaggatttgc gtcgcgagcg gggctactcg acggggttgg ccgagctgga cgcgctgctc
     3721 ggggggctga tcccgggcga caacatcgtc tggcaggtcg atcagctcgc cgattacgag
     3781 gcgttcgcgc ggccgttcgc ggcgggggcg gtcgcggtcg gccaccgcac gctctatctg
     3841 cgcttcgccg accacgcccc ggtgctggcc gacgacgcgg gcgtcgagcg cgtcgagctc
     3901 gatccgcagg ccgggtttga aatgttcctg ggcgcgatcc acgcgacgat cggccgcgcc
     3961 gggcgcggcg cctgctacct cttcgattcg ctctcggatc tggccgtcga ctggttcagc
     4021 gaccagatgc tggggaactt cttccggctg acctgcccgt atctttacga catggggacg
     4081 atcgcctatt tcgcgctgct gcgcgactac cattcgctgc acgcgaccgc gccgatccgc
     4141 gagacgaccc agcttttcct cgacgcctat cgccacaagg gggaactcta cgtccggccg
     4201 cgcaaggtgc agcagcgcca cacgccgggg atgcacctgc tgcacgcctg gcgcggcggc
     4261 cggttcgtca gcgtgaccga cagccaccag gcggcggaga tcatgacggc ctcgccgcgc
     4321 tcgacgctcg acaccgcgat ggaccggctc gacgtctgga accgcgcgtt cctgcaggcc
     4381 gaggaggcgc tgcgcgatca ggaccgcctc ggcgcggccg atcacgatct ggacgagttg
     4441 cacggccggc tgctgcggat ggtgatctcg cgcgatgagc ggatgctgcg gctcgcccgg
     4501 cggcacctga gcttggagga agtgctccag atcggccggc ggacggtcgg caccggcctg
     4561 atcggcggca aatcggtggg gatgctgctc gcccgggcga tcctgcaacg cgccgacccc
     4621 cgctggcgcg accggctcga ggtccacgat tcgttctaca tcgcctcgga cgtcttctac
     4681 tcgttcctgg tgcgcaacgg ctgctggtgg atccgccaga accagcgcaa cccggcgacg
     4741 ttcctccaag gggtccacat cgcccgccgg cggatcctgg aggggacctt ccccgaggaa
     4801 gtggagcgcc agttcgcgga gatcctggac tatttcgggc cgtcgccgat catcgtgcgc
     4861 tcctccagcc tgctggagga caacttcggc aacgcgttcg ccgggaagta cgagagcgtc
     4921 ttctgcccga accaggggac gctcgagcag cggctggaga atttcctggc ggcggtgcgg
     4981 acgatctacg ccagcacgat gcgcgaggag gcgctcaact accgcgcgcg ccacggcatc
     5041 ctcggccgcg acgagcagat ggcgctgctc gtccagcgcg tctcggggtc gctgcacgac
     5101 ggctacttct atccgcaact ggcgggcgtg gcctattcgt tcaatcccta tgcgtggagc
     5161 gaccagatcg atccgcgggc ggggctggtg cgcctggtgt tcggtctggg gacgcgcgcg
     5221 gtcgaccgga ccgacgacga ctacacgcgg atcatcgcgc tgaactcgcc cgagcgccgc
     5281 ccggaacgct cggaggaggg ggtcgggcgc ctcgcgcagc agaaggtcga cgccttggac
     5341 ctgacgagca atgcgctggt cacgaccgac ttcagccgga tcctggcgca gcggccggcg
     5401 atcccgatgc acctgttcgc cagctacgac cccgaccgcg ggcgctacta ccgcgagcgg
     5461 gggcggagcg acggcgaccc gcgcgcgctg acgttcgacc gcctgatcgc cgagaccagc
     5521 ttcatcgacg atatgcgcga gatgctgaaa accttgcagg aggcctatgc ctaccccgtc
     5581 gacgtcgaat tcacggcgaa ttttctcgat gagcgcgaat acaagatcaa catcgtgcaa
     5641 tgccggccgc tgcaggtgaa gggcgggatg gtggcgatgg cgctgcccga cgacgtcgcg
     5701 ccggagcaga cgctgctgcg gacgcgcggg ccggtgatcg gtcacagccg cgtcacgccg
     5761 gtcgaccgcg tgatcctggt cgtgccggcg gcctacggca acctgccggc ggccgagcgc
     5821 tacgccgtgg cgcggctgat cggccggctc aacgcggtca acgacaagga gacgacgctg
     5881 ctgatcgggc cggggcgctg ggggacgacg acgcccgcgc tggggatcac ggtcagcttc
     5941 gccgagattg atcgcgcgcg cgtggtgtgc gaaattgtcg cgatgcgcga ggatctggtg
     6001 cccgacgtct cgctggggac gcatttcttc agcgaactgg tggagatgga aatgctctgg
     6061 ctggcgctct ttccgacgca gtcgggcaac ctgttcaacc aggcgctgct ggaggcggcg
     6121 cccaaccggc tgaccgatct ggtgcccgac gcggcgcatc tggcccatgt tgtgcgtgtg
     6181 gtcgacgcgg aggatctggc cggccgcggc cagctccggc tgcacgccga cacgctgaac
     6241 cagcgggtgc tgtgctacct cgcccgcggc ggctgaagga tcggtgttgc aatcggaatc
     6301 gctatcggga tcgccatcga gctcgccatc gggctcgggc tcgattcccc ccgcatcccg
     6361 cggggatgcc agagaagagc cggtggtcga gcgccgcgac accaccggtc agcggccgcc
     6421 caacacccgc atcccgcggg gatgccagag aatagccggt ggtcgagcgc cgcgacacca
     6481 ccggccagcg gccgcccaac acccgcatcc cggcagggat gccagaaacc atgcggcgcg
     6541 tcaacggacc gattcaaatg accgaggcct tgccgcaccg atcaaccggg cagtcccgtc
     6601 agggacggca gacttcagcc cgccacttca gtggcgggaa acgatgaagg aaagaaccaa
     6661 gagtcccgca gggacggcag aacccctcga atcgatctcg atctcgatca caaccacgac
     6721 aacaaccacg aacgacgcaa aacaaaatac gtatcacaga gaaaggacgg gatcatggaa
     6781 cagagagcgt tgggacggac gggcgatgag gtcacgatgc tggcgttcgg cgcggcgcag
     6841 ctcggccaac tgatggagga tcaccaggcg gccgccgcgc tggtcgacca gttgatcgat
     6901 gccggaattc ggttcatcga cacggcggcg tgctacgcca attccgagga gaagctgggg
     6961 ccggtgatgg cgcgccgccg cggcgaatgc ttcctcgcct ccaagtgcgg ccatcaggtg
     7021 gatcccggag atccgccgga gtggtcgccc gagatcatcc gccatagctg cgaacgctcg
     7081 ctccgccggc tgcaaaccga tcatctcgac ctgctgctgc tccacacctg ctccgaggag
     7141 aaactccgcg acgaagcgat gatcggggcg ctcgagcaat gcaagcggga tgggttgacg
     7201 cggttcatcg gctacagcgg cgacaaccag tcggcgctga ccgcgatcaa gtcggggcgc
     7261 ttcgactgcc tggaagtcac gatcaacttc tgcgatcaat cggtgctgga cgaggtgctg
     7321 ccggcggcgc gcgcggcggg gatgggcgtg ctggcgaaac ggacgatcgc caacgcctgc
     7381 tggcgcgagc tgtcgcggct gcccgcaaac catcaggcct acatccggcc gtatgtcgac
     7441 cggctcggga aactgggatt tacgccgcag tcgctgggct acgacggcga ctgggccgag
     7501 ctggcgctgc gcttcacggc gtttcagccg ggcgtcagca cggcgatcat cggcggaacc
     7561 catttcggcc acatccagga gaacatccgc gccgcggcca tgggaccgct gccggccgac
     7621 gtgcaggccc ggctgcgcga ggcgtggcag gccagcgccg acgcgtcgtg gatcgggctg
     7681 agctgaggca cggttcaggc tccggcgctg ccgcgcggcg gcgccgcgta ttatgaaacg
     7741 aaatccgtcg gatccgaccg atccgacgga ttttatcatt tggagccctt agggttcact
     7801 tgaaacctgg cgtcgaatcg ccgccgcccc gttcaagcgg cattcatccg cccgaagtca
     7861 cggcttgcgc ctcctggagc gcgcggatgt agtcgcgagc cagaatgaga tccttgagcg
     7921 cctcttcggc ggtgcagggg ggttcgatct cgccgcggat cgcctggcag aaggcgatgg
     7981 cctggcggcg catggcgtgt tcccacggca tctgcggtcg gcggcgctcg ggcgtgcccc
     8041 ctttcggatc gtagcagagc tcgaccgacc ctgcccggtt gaacgccagc ggggcgggca
     8101 ggtcgagccg cagccagccg cgctcaaaac agatcagcgc gctctcctgc cagtcgatcg
     8161 agttggtcca cggcgccatt tcgaggacgc aggggaggcc gccggcgctg cgcccctgca
     8221 ggaccacgcc ggtcggatcg acggagacga tctcccagtc ctcgccgagc aggtggcgga
     8281 tcagattcac ctggtggatg tagccgttga cgaaaccgag ataggctcgg tacatggggg
     8341 cgtccatgtc ggcgggcggg ggttcatgct cgagcgcccg gacggtctcg tcggaggtca
     8401 tcatgccgtt gaagccgttg gcgacccagt cgcccggcgg catcgtgacg cggaggtacc
     8461 gcagcgggcc cagctcgccg gtttggcgca ggcgcccgat ttcgccgtgg acccactgcg
     8521 tcgccgggtc gcagcgccgt tggtagccga ccatcagcca ggcggggctg gcgcggaccg
     8581 cctcgacgat ccgctccccg gccgggacgg acgaagccag cggcgcttcc gtcagcagcg
     8641 ggacgccggc cttgagcagt tctggcaaca gcaggccatg gcgctgaaac ggctgggcgg
     8701 cgacgatgcc gtccagcgct tcgtgcgcca gcagctcgcg atgggtcgga taaacgcgcg
     8761 cgacgccatg gcgggcggcc acctggcggg ccatctcggg gcgcagctcg gcaagcgcaa
     8821 cgacctcgca atcgggcagc gaggcgaagt tgcgcaggtg cgcgcactgg cccatcgcgc
     8881 cggcgccgac gaaaccgatc ttcagcttgc ggttcatccg ggagggtcct ccgtgacttc
     8941 ggtcgggaag cggccttccc ggatcgacgc gacgacggcg gggggcgtcg cactcgataa
     9001 cgagaagccg gctgcggcga acaggccggc atccggttgg gtttttatac aaagtgcacg
     9061 agacagggat cgcgagacat gcgtggaatg cggcatccct cccctgcccc gatgatcgta
     9121 tgcctcgcat caataggctt cggcgggcgg aaagtcaagc gccgcggaat gggattaatt
     9181 ccttacgtat cgaagattcg catcgaatga ccctctgcca gtcccgtcag cgacggcaga
     9241 cttcagcccg ccacttcagt gcttttcatt tcccaaatct caatccaccg ggggctcaaa
     9301 tcctgtgcag gcgatttcga ctcggttgcc ggctgagtcc gcgaagcgga cgactggaat
     9361 aaagcgcagg gtgaaggccg aaggccggaa ccctgcgtgg ccgtctccaa cgatccaagc
     9421 ccctggaagg ggcgaccgca gctggtctgc attcggggaa cgacaagcac ttcagtggcg
     9481 ggaaccctga aagtgagggc gaaagaatcc cgcagggacg cacaaccgct cggtttgatc
     9541 gcgagctcca gaaccgaggt gcatatgctc gaacaaatgc aattcggccc ggaacggcgg
     9601 ttttccgctc cgggccgagg tttcttccac gggatgactt attgatacag ttcccacgcc
     9661 tgggcggcgt tgcccacctg caggatcgaa ctgatcatgc cggggttgcg cagaccgccg
     9721 cggaagccgt tccacgtctt gccggcgggc acgacgggcg cttcggtctc gacgacgcgg
     9781 atccggccat cgtggctgcc gaagacgacc tccacccgtc cgtcgaagtc gagatcgccg
     9841 accgacccgg cggtggcgat gagcgtcgtc gcctgagcca tcaccgccgg ctgatcggcc
     9901 ttgggtccgc tctcgccgca gacggcgtag aggctgccgc tgctcgaacc gatgagcggt
     9961 tcgggcgtgc cgtcgccatc caggtcggcg aagaacggcg aggcgaagat ctgcgactgc
    10021 gtggcgacag ggaagccggg cagcgtcttg ccgtcggcgc tgaaaccgta gaggttttca
    10081 tcgtacgagc cgacgaagac ctccagctgg ccgtcgccgt tgacgtcggc cagcgacggc
    10141 gacgaggtgc cgccgtagcg gcgctgggcc ggccagccgg gcttgatctg cccctcggcg
    10201 gtcagcgcgt aaacgggggc gtcgctcatc gcgatcacga tgtcgatctg gccgtcgcct
    10261 tccaggtcgc cgacggcggg gctggcgagg acctcgccgc ccaggtcaac cggccacccc
    10321 ggcatgacgt cgccggccca ggtgatcgag aagaccttgc ccgagaggtt gacggcgatg
    10381 atggccaggc cgtcgccggc gggcagcggc gccagcgcgg gcgagggggt catgaagtga
    10441 tgcgagccga tatcgcgcgt gccgcccagg tccaccggga agccgggccg gatggagaag
    10501 tcaaagccga gggcatacag cagcccgtgg ccggtctgca cgatgatctc cggatcgccg
    10561 tcggcatcca ggtcgcccac ggccggcgtc cccttgatcg tcccttcgag gtacagccgc
    10621 tcgccggccg gggtcagctc gacgccgtcg tgccggtagg ccaccagcgc gccgttgtcg
    10681 tagccgcgga tcacgtcaag caggccgtcg ccgttgaggt cgacgacgac cgggctgcac
    10741 tgcgaatcga ccaggatctc ggggaacgga tcgatgacga cttcgctgtg attgaaggcg
    10801 ttttcgatca cgatcaggtt gaggtaatgg ccgtagacga tgtcgacgaa gccatcctgg
    10861 gtcatgtcgg caaggacggg ggtcgccggg atggccgatt cgagcttctg ctggaagagc
    10921 aggccgtcga cggccttcgc cgccgcggtc cgcttcagcg ccttcgccag atcgatcggc
    10981 caggtcttct cgtcgacgac gttgaccagc ggccagttct ggttggtcgt catgcccgac
    11041 ttgatccacg gctcgaagtc gaggtgggat tcccagatcg gcgtgtattc aacggggtat
    11101 tcgaacgccg tgggcagcag catggcctgc ggcagcggga agccgatcag cggcgacgcc
    11161 ggccactgcg agccggaaac gatgtccagc gtcgcggtgt tgtgatccca gccgtggtag
    11221 atcgtcgagc cggaattaac gacgtagagg ttgtagccgt agatcgccga gttgtgctcg
    11281 tcgagcgtcg tggccggatc atcgacgatg cgttcgttgg ccggggcatt gcgcgggcgg
    11341 atcgtgatca gcgcgtagtg cccttcggtc cgctcggcca ggctctgctg ctcttcggtg
    11401 ttggtgagct ggccgccgcc gccggggaac ggcgggagcg catctttggt cagcgggaag
    11461 atctgcaggc tgcgggcggg gttatcggcg ctgacgaaga tgtcctggct gacgaggaca
    11521 tcgttggcgt cgaagcgctg aatcgtggcg gtcacgcccc cctcgagccc gagatcgagc
    11581 cgggcgatat gattgaagat cgcgccgcgg gccagcgctt ccacgcgcat ctcgatcagt
    11641 tcgagcttgt tgttcgcatc ataggtttcg gtcacgtccg accgcatgac gaagtcgtta
    11701 tattcgaagt cgttgtagag ctgctgggcg actttcaggt cctcgaagac gatcagcgtg
    11761 gaggagctca tcgtcttgag cggcgtgatg tcgctggcgg tgttattggc cggattcgga
    11821 tcggcgccgt tggcgccgtc gtcggcgatc gtcaccgtgt tggtcgtgac ggtgtagtcg
    11881 ctgggcagcg gatcgagcat gttgacggcg aagaccagcg gcgccggcgg ctcccacggg
    11941 cccacgccca gatcgccgac gtcgaaggta tagacgccgc cgccctgatc catccacccg
    12001 ggcgtgctgg cgccggcatt gaacgtggtg aacgccggca gggtctcggt cacctggacg
    12061 ccggtcgcgt cctggttgcc ctcgttgatg tagaagatct cgtagctgac gagctggccg
    12121 ccggcttcga cgcgcgcgct gttgtcgtcc ttgcggatgc gcaggtcggg ctcggcgatc
    12181 agcggcgtcg tgtccatcgc catcgcgtcg tcgatcggca tgacgggctg ctgaccggag
    12241 taaacgtcat cgatgacgac gacgttctcg atctggatgg cgcccgccgg caccgggttc
    12301 tggacggtga aggcgatctg cagcacgccg gtcgtatagc cgcccagcag gccgacgtcg
    12361 tacgtgacca ccggacccgc gctgacctcg tatgcggccg gttccagctc gcccggctgg
    12421 aaccggccgc atacgaggtc agcgcgggga tggtgtcggt gatccgcacc tcgggcgagt
    12481 cctggttgcc caggttggcg taggcgatgg tgtagaccac ggtgccgtcg ggctggacgc
    12541 tgacgccgcc atccgtcttg ctgatcgcca gctcgacctc ggcatcgacc ggcgtggtgt
    12601 cggtggagct gttgtcgtca gggttcaggt cggggccgcc ctggccgtca tcactgggtg
    12661 aagtgatatt ggtgatgatc tcggtgaacg gaccgaccgg atcgttgaca tcgacggcga
    12721 agatcagcgt gccagtggtg ccgacatcca ggcccccgac ctcgtagcgg tagacgccgc
    12781 cgccctggtc gacccagccg ggagaactgg ccgcgccgtt gaagaccgtg tcggcgggga
    12841 ccgattccat gatgacgacg cccgtggtgt tctgatcgcc gtgattgccg taggtcagca
    12901 aatagctgat cgtgccgccg gggatgacgg tgacgccgcc gtcgtccttg tcgatgctca
    12961 gatcagggcc ggcgatgacg ggcgtggtgt cggaggtcat gttgtcgtcg agcgagaatt
    13021 cggggccctg cgagccatcg tccatgatcg cggcctcatt gacgatcaac tcgacgccga
    13081 cgggaacggg gttgaccaca tcgacggcga agatggccgt gccggtggtg ccgccgtcca
    13141 gatcgaagac gttgaatttg aactggccgc cgcccagatc gatccagccg ggcgtgctgg
    13201 ccgccgggtt gaacacggtg tgctcgggca gcgtctccag gatcatgacg ccggtggtgt
    13261 cctgatcgcc gaaattgccc cagaggagcg tgtaggcgac ggtgccgccg gcgccgacgg
    13321 tcgtgccgcc gtcgtccttc agcaggctca gatcggggaa cgccgcgacg ggcgtctgca
    13381 tgtcaaaccc attgtcgtcg gggttggcat cggggccgtc ggcgccgctg tcggcgatcg
    13441 agacggtgtt gaagatgaac tcgacgtact gatcgatggg gttgtcgacg tcgacggcga
    13501 aggtcagcgt gccgttcgtg ccgccgccat cgagcgtgcc gatgtcaaac gtgaagacgc
    13561 cgcccccctg gtcgacccag ccgggcgtgc tggccgcggc gttaaacgag gtgttcggcg
    13621 ggagcaattc ggtgacgacg acgccggtcg aggcctgatc gccgttattg gcgtagaaga
    13681 tttcgtaact gatcgtctgg ccggggacgg cggtggtaat cccgtcatcc ttgctgacgg
    13741 cgagatcagg catggcgatc agcggcgtgc tgtcggcggc cggggcgctg ttggcgtcct
    13801 ggtcggtgat tgtcgcgctg ttgtcgatcg acgtcacccc cgagggcacg gggttgtcga
    13861 cggtcacggc gaacaggacc gttccggtgg tgtcgacatc gagcgcgccg atcgggaagg
    13921 tgaattgacc ggggttgact tcgatccagc cgggcgtgct ggccccggcg tcgaaactgg
    13981 tgtgcgccgg cagcagatcg gtgatgacga cgccggtcgt gtcctgatcg ccggcgttgg
    14041 tgtagaacag ggtatagatc acggtcccgc cgggtgtgac ggtcgttccg ccgtcatcct
    14101 tgctcaggaa gagcagcggg gaggcgtcga gcggcgtcgt gtcgctggtg atgttgtccg
    14161 cggggttctg atcggggccg ttgctgccgt cgtcgtcaat ccgcgcctcg ttgatcagtt
    14221 cggtcaggcc aaggggggcc ggggagttga cgtcgaaggc caggttgagc gtgccggtgc
    14281 cggtggtggg caacgcgccg acggcgaagg tgagcgtgct gccggcgtct cccgagccgg
    14341 tcagcaccca gccgggcgag ctcagcgcgg cgttatacgt ggtgtgctcg gggacgacat
    14401 cggtgatctc gacgccggtg gcgtcctggt cgccgttgtt ggcgtaatcc agcgtgtaga
    14461 gaatggtgcc gccgggcgtg gcggtggtgg cgccgtcgtc cttgatcagt tcaagatcgg
    14521 gggccgcgac gatgacggtg gtcgtcggat cgtccagagc gaccgtgtcg ggatcatcgg
    14581 tcatgctctg aacgccgccg ctggtgaccg tgccctgctg ggaaatcagc tcgacgccgg
    14641 cgccgagcgg gtcggcggcg gcgacgtcga aggtgattgt gacggattta ccggcgggga
    14701 tcgagcccag gttgagggaa atctgcgccg gcgcggtggt caccgccagc aggctcaaca
    14761 gggttgccca aaggagcttc cgcttcatca cggtaccttt cgcggtcaaa catcaatcgg
    14821 gtcggattct gatcccgcag gcgaccgtcc gcaagtcggg agtcgattca gccagacgat
    14881 tcagttgcct gaggtttcat tcagtggaaa ctatggaaca atgccccctg aatgtcaatt
    14941 tgtccctccg gaggcggccc ggcgacgccc ccggtcgacc tcgcggtcgc gagagcccgc
    15001 cggggcgctc ggctcgaccg gccgtgagcc gccgaaccgc ccagtgtttc gctctgtcgc
    15061 catgctgttg gatcgctcca tgagccaggc tgttcatccc atcagggatc gacgggcggc
    15121 gtgtcgcagt cggcatcggt atagttgaca tcggaccgcg gcagggaagt caccacccag
    15181 gtgtcgggcg tcccctgatt gcgtccattg atgaaagcct cgaccaccgc ggcattggtc
    15241 ccggagaggg cgcccagccc gtccagatgg aacgggttgt cgccgggggc ggcgccgaaa
    15301 cgctgcacga gttggtaatc gggcgcgccg ccggcggttc cgtcgttgac ggtgttctgg
    15361 gcgatatcga tgcagagcgt ctggcgatcg gtaacggcgc cgccgccgcc gagcatgttg
    15421 atgccgcagc cgggttcggt gcccgtcatc ttgattgtat tgttcttgat cgtcgcctgc
    15481 aggttgccgt tgactccctc gcgggtttcg acccgaatcc cctccagaaa accgccatcg
    15541 agattgatcg tgttgttgag aatttcaacg agcattgtgc cggcgccgcg ctggacgacc
    15601 tgaatccctt cgccgaaccc ttggagggga atcgttacgc gattgccgct gatcgtgccg
    15661 ttcaggcggc agttggcggt tgcatcggcg agcatttcga cgttgatgcc gtcagcgaag
    15721 ggcgtcagta cagtcccgaa ggggcaatta tcccgaatct cgaacgcgac ttgggcatcc
    15781 tggtcggcgg tgagtcgcga aaagtatttc gtgccttccg ctcggtttga gacgatcgcg
    15841 acgttgccca gggccgaccc gctgaaatcg aagtcgattc ccgtatcgaa ggttgacccc
    15901 agatcgttcg atgtgatctg ggcgtcggcc aggacatggt cgccgctgcc gacatacgac
    15961 ataaccccaa ccaggttcag actcatccgg ttgccatcga cggtaagcaa gtcgacggtc
    16021 cccgtcggat tcagaatcga gatcccaaaa cgcttattga gattgatgta gctctccttg
    16081 a
//
LOCUS       DAOPCO010000060        16037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3703_length_16037_cov_5.954574, whole genome shotgun sequence.
ACCESSION   DAOPCO010000060 DAOPCO010000000
VERSION     DAOPCO010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 16037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16037
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_16037_cov_5.954574"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2596)
                     /locus_tag="PLS90_06335"
     CDS             complement(<1..2596)
                     /locus_tag="PLS90_06335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014160316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK02058.1"
                     /translation="MPLTTFHPTIQRWFRDRLGEPSAPQRAGWPAIRGGAHTLIAAPT
                     GSGKTLAAFLTALDGLLRAPEPLPDKTRVLYVSPLKALGNDVQKNLQAPLAELRELDP
                     ALPAIRVLVRTGDTPAHERAAMGRRPPHILVTTPESLYLLLTSDGGRALLRDVRTVIV
                     DEIHAVLGDKRGAHLALSLERLEALTERPPQRIGLSATQKPLAAVGAFLVGTHRDCTL
                     VDQGALRALDLGIELPSSPLETVCSHETWQEIYTRMAELIEAHRTTLVFVNTRKMAER
                     VAAQLSRLVGPEQVACHHGSLSRERRLAAEQRLKHGQLRALVATASLELGIDIGEVDL
                     VIQVGATRSIATLLQRVGRSGHGLGRTPKGRLNPLTRDELVEAVALLSAIRRGELDRT
                     PQPRRPLDILAQQIVAACVPEAWNATELFERLRRAWPYRELTRAEFDAAVELHTGGRR
                     ALLHRDGVHGRLRATRRARLTAITSGGAIPDAADYRVIMQPEEIFVGTLNEDFAVESN
                     AGDIVQLGNASWRILKVEPGVVRVADAQGAPPTLPFWLGEAPARSAELSDAVSRAREA
                     AEIPGRLAEEAGVPEDTARLVAEYIEEGRRVLGAVPTRRRIIAERFFDESGGMQLVIH
                     APLGSHINKAWGLALRKRFCRGFGFELQAAANDEAILLSLGPQHSFPLQDVFNYLRPE
                     TARDVLIQALLATPLFETRWRWNVARALLVPRFERGRRLPAPLARMRAQDLLVEAFPE
                     VLACGENLPPGDLPVPLEHPLVRQTIEDCLEEAMDVEGFLQVLREIRDGTIETLAIDT
                     PTPSAFAHGILNAQPYSFLDDAPLEERRTQAVLRRRGLDHRTADELGELDPAAVARVR
                     EEAWPDPRD"
     gene            complement(2718..3719)
                     /locus_tag="PLS90_06340"
     CDS             complement(2718..3719)
                     /locus_tag="PLS90_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK02059.1"
                     /translation="MGLRVIIPVAGVGTRLRPHTHTAPKVMLQVAGKPMIGHILDELR
                     KYDVEEITLVVGHLGDAIRDYVTRAYDFKFQFVTQEELKGLGHAIWMTGDLYRKVQSP
                     LLVILGDTIFDADFKAIIGSDSNYIGVKEVEDGRRFGIVELEGDQIVSMVEKPEHPPT
                     NLAIVGIYYFTDGRMLYKCLDEVVEKGVTTKGEIQLTDALALMLKQGARMKPFVIEGW
                     YDCGKPETLLSTNQILLSKNTTATNGQPEERNGSLIMHPVSIGKDAVLENSIVGPYVT
                     VAEGSTIRNSVIKNSIVSSHCLVENMLLDASIIADHAKVQGTYRQLNVGDSSEIRLG"
     gene            complement(4001..4435)
                     /locus_tag="PLS90_06345"
     CDS             complement(4001..4435)
                     /locus_tag="PLS90_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPK02060.1"
                     /translation="MMSRSLYLSGGMILLLLAAGCGVNTGVSYRIPGTGVSVGASAPL
                     DRVGKVDSAENAVYRQIELDSEPAGGRVLVNGRLVGATPMTVTLPFKKGFMGSPKGMV
                     QVLVEKKGYLPQGVDLFPRGKNVALSPEGDPVRKVTIKLTKE"
     gene            complement(4705..6063)
                     /locus_tag="PLS90_06350"
     CDS             complement(4705..6063)
                     /locus_tag="PLS90_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HPK02061.1"
                     /translation="MLEPYEILETIRMITVEHLDVRTITLGISLRDCADSDGARARRR
                     IYDKITRAGENLVAVGEQIAMEFGIPVINKRIAVTPIAMIAAACPDEDYVPFAETLDR
                     AAAVVGVNFIGGFSALVQKGYTTGDRRLIDSIPAALAATERVCASVNVASTRAGINMD
                     AVAHMGRIIRATAERTRERDAIGCARLVVFANAIEDNPFMAGAFCGPGEPELAINVGV
                     SGPGAVKAALEKVRGESFDVAAEAIKQVAFKITRVGQLVAREASRRLGIPFGIVDVSL
                     APTPTVGDSVAHILEELGLERCGAPGTTAALALLTDAVKKAGIMASSHVGGLSGAFIP
                     ASEDAGMIDAIRVGALTLDKMEAMTSVCSVGLDMIAVPGDTSAETLAAIIADEAAIGV
                     VNHKTTAVRIIPAIGRAAGDTVQFGGLLGEAPVMPVSRFSSAAFIARGGRIPAPILSM
                     RN"
     gene            complement(6138..6407)
                     /locus_tag="PLS90_06355"
     CDS             complement(6138..6407)
                     /locus_tag="PLS90_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPK02062.1"
                     /translation="MRAIVTVIGHDRVGIIAAVSTTLSRHQVNILDIRQTVMQEYFTM
                     IMLVDLAAMSLSFPELVAALNATGEQLGVDVRIQREETFQAMHHV"
     gene            6703..6882
                     /locus_tag="PLS90_06360"
     CDS             6703..6882
                     /locus_tag="PLS90_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02063.1"
                     /translation="MLYFGQILDRVERGERVAVLRNGRHVATIIPARPAPDAVKQYLT
                     GFMLGANDSGRWGEV"
     gene            complement(6980..7309)
                     /locus_tag="PLS90_06365"
     CDS             complement(6980..7309)
                     /locus_tag="PLS90_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPK02064.1"
                     /translation="MATRPSYDFEMSIGHPFTVGILHIDLHLPCCHSLKEKRGRLAKV
                     MNHLRKKHPVVVAEVGDQNTWDRAGLAAVTLSPSRDLANRILDAAADTLAKDGEIELV
                     WHEIELV"
     gene            complement(7618..>8451)
                     /gene="cobA"
                     /locus_tag="PLS90_06370"
     CDS             complement(7618..>8451)
                     /gene="cobA"
                     /locus_tag="PLS90_06370"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPK02065.1"
                     /translation="AARGLAGQPAGAAEPPANGRAGGRLTLVGAGPGDPDLITVAGRD
                     ALRAADVVIYDRLVHPELLRHAPASAVRVYLGKRPGEPHQPTQDRIHDLIVEHGRAGR
                     HVVRLKGGDPMLFGRGGEELEVAARAGIPARVIPGVTAALGAAAAARLPLTHRELASS
                     VAFVTGCGAAHRGDPGVDWAALARAVDTIVIYMGVEHLTGIAAALVAAGRPAGQPVAL
                     VCRATLADEAVFVAALGDLAAGLTPAGVEAPAAIIVGEVARCREASRQVAAAGVPAAA
                     G"
     assembly_gap    8452..8551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8552..9184)
                     /locus_tag="PLS90_06375"
     CDS             complement(<8552..9184)
                     /locus_tag="PLS90_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02066.1"
                     /translation="MPYLPINVQLEGAHVVVVGGGRIALQKARVLAAAGAALRVVAPR
                     ILPAISALPGARCHPRPFREEDLDGALLAIAATDDRALNLRVYAAARARRVLVNVVDD
                     PTHCDFIFPSILRRGELAVSVSTAGAGPALARMLREELEAEFPEDYGAALTRLRELRR
                     TLRRELICPGAVRAGAEALSAMAARARRPDRLADELARMQALADELTAAAR"
     gene            complement(9187..9630)
                     /locus_tag="PLS90_06380"
     CDS             complement(9187..9630)
                     /locus_tag="PLS90_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK02067.1"
                     /translation="MLVTQKCQYALRALFELALQGNHKPVKIALVAERQRIPFRFLEV
                     ILSQLKQGGFVESRRGTEGGYLLARRPEEITVGDIVRFIDGPQIEDEQARLEQSDPPG
                     EVDLFGPIWDQARAAVFQVFDGLSLADLLAQYEASTRRFVPNYVI"
     gene            complement(9898..10173)
                     /locus_tag="PLS90_06385"
     CDS             complement(9898..10173)
                     /locus_tag="PLS90_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02068.1"
                     /translation="MDVFECLKILRIGRIQVISNITVHRSVRGSGLLRCDRSDGAESG
                     MVRVSDGLVGVQVSAGLHQALIHLIRICIHRLPSGIPPRDAGEVTAG"
     gene            complement(10182..10301)
                     /locus_tag="PLS90_06390"
                     /pseudo
     CDS             complement(10182..10301)
                     /locus_tag="PLS90_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007806254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(10411..11247)
                     /locus_tag="PLS90_06395"
     CDS             complement(10411..11247)
                     /locus_tag="PLS90_06395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPK02069.1"
                     /translation="MTSQAGRWSRAELLVVFRLYCRTPFGRLHRRNPEIVALAQRIGR
                     TPDAVAMKACNFASLDPQLRERRISGLGNASRADRALWEEFAADSAAVALAAESAFAE
                     LTGLEDETTVPELGPAGQSAPRGRPTKPRERVSKPPPPVGPTEAEALVRVRRVQRFFR
                     AAVLAAYGGRCALTNLAIPDLLNASHIIPWHADEKRRADPRNGICLNVLHDRAFDRGL
                     ISFDDELRIIVSDRLREAEGELCELHRDHLLGLAGKKLRLPDRFLPDAAAIAYHRRKV
                     HG"
     gene            complement(11369..12457)
                     /locus_tag="PLS90_06400"
     CDS             complement(11369..12457)
                     /locus_tag="PLS90_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02070.1"
                     /translation="MKLPLPRLSLAVLALLLLAPGCRQRPYLETVFQTPYLGFPGGLD
                     NLLRLDVNRLRGRDWYRETLREARQSPRWGLVIQGFTEQVQYQPWQEIDELYVGIRGE
                     VTADQPFRNAVFIARGRFERPAETLERLRLWLGQEFLIDPPPFRQSTYGDGWPVFQTS
                     GQSQYRESVVHELSFAFPTLPAAGDNAPPPPTLMVFGLNRGLFNDTLAVMAGEVDGLQ
                     KDAEWLALLQRPNIGALAWGTGRTHNLKFAAVRELLGRAQAYFFHLDFESSNLKAEVG
                     LVSGSIDQAQALTDLAQQKQQLLVAAWQPIAPVAPALAEVPGKLFIITEAETTSIRVS
                     LTHLEWRAVRDDFKRIRSTTQSLPLPPL"
     gene            complement(12957..13370)
                     /locus_tag="PLS90_06405"
     CDS             complement(12957..13370)
                     /locus_tag="PLS90_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02071.1"
                     /translation="MGWRQRLRIGPGEIIILMTIGILALIVITGNLRNLRAILNWLYS
                     PYAAFIVVIMLVEYLLLKGADRSHIYRRELDAARQKRRDDLLALRQIETELVDLRARL
                     GAELENPTEPGRLADTLDHSRSTVERVLSLIRDRI"
     gene            13594..14382
                     /locus_tag="PLS90_06410"
     CDS             13594..14382
                     /locus_tag="PLS90_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPK02072.1"
                     /translation="MKQVSVELIRNETLIPGTVLHHYRDAEIAATARPGQFVNIRPGY
                     GSDPLLRRPFSICTVDRSAGTFTVLIKEVGAGTRMLLALRSGARVDMLAPLGRSFAWD
                     GARRLLLVAGGVGVAPLLFVANEARARETGTEPAPEVIFCYGARTAGDFVLLDQIRPV
                     ASRLVLTTNDGSLGEAGFVTAAAERFFTPETTILTCGPNPMMDDLLRRMRTAGLEGQV
                     SLENQMGCGIGACQGCVVPTRRGYVRICCEGPVLRTDELDEIRF"
     gene            complement(14867..15580)
                     /locus_tag="PLS90_06415"
     CDS             complement(14867..15580)
                     /locus_tag="PLS90_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPK02073.1"
                     /translation="MFFDPGYLLFVFLPTLILAGGASLWVKSAFSKYSKVRASSGLTG
                     AQAARAMLRAAGMDRHVSIERVSGHLSDHYDPRHKVLRLSPAVHDQASLAAVGVACHE
                     VGHALQDAQAYKPLVLRNAIVPTAGFGSNFGMILVMIGLGISTLTQSALGFYIAMGGV
                     LLFGTVVVFQLVNLPVEFNASARAKQMVVQLGIVQPGREAGAVASVLNAAAMTYVAAT
                     VTAILQMLYFLMLVMGRRN"
BASE COUNT         2587 a         5128 c         5718 g         2504 t
ORIGIN      
        1 cgtcgcgcgg gtcgggccag gcttcctcgc ggacacgggc gacggcggcc gggtcgagct
       61 cgccgagttc atcggccgtg cggtggtcga gcccgcgccg gcgcaacacg gcctgcgtcc
      121 ggcgctcctc cagcggggcg tcatccagga aactgtaggg ctgcgcgttg aggatgccgt
      181 gggcgaacgc ggaaggagtc ggcgtgtcga tcgcgagcgt ctcgattgtt ccgtcgcgaa
      241 tctcgcgcag cacctggagg aaaccctcga cgtccatcgc ctcctcgagg cagtcctcga
      301 tcgtctggcg cacgagcgga tgctccagcg ggacgggcag atcgcccggc ggcaggttct
      361 cgccgcaggc cagcacttcc ggaaaggcct cgacgagcag atcctgcgcg cgcatgcgcg
      421 ccagcggggc gggcagccgc cggccgcgct cgaaacgcgg cacaagcagc gcgcgcgcga
      481 cgttccagcg ccagcgcgtc tcgaacagcg gcgtcgccag cagcgcctga atcagcacgt
      541 cgcgcgccgt ctccggccgc aggtaattga agacgtcctg cagcgggaaa ctgtgctgcg
      601 ggccgagcga gagcaggatc gcctcgtcgt tggccgccgc ctgcagttcg aagccgaacc
      661 cgcggcagaa ccgtttgcgc aacgccagcc cccaggcctt gttgatgtgc gatcccagcg
      721 gggcgtgaat gaccagttgc atgccgccgc tctcgtcgaa gaagcgctcg gcgatgattc
      781 ggcggcgcgt cggcacggcg cccagcacgc ggcgcccctc ctcgatgtac tccgcgacga
      841 ggcgcgcggt atcctccggc acgccggcct cctcggccag gcgcccgggg atttcggcgg
      901 cctcgcgtgc gcggctgacg gcgtcggaca gctcggcgga gcgggccggg gcctcgccca
      961 gccagaacgg caacgtgggg ggcgccccct gggcatcggc cacgcgcacg acgcccggct
     1021 ccaccttcag aatgcgccag gaagcgttac caagctggac gatgtcgccc gcgttggact
     1081 cgacggcgaa atcctcgttg agcgtgccga cgaaaatttc ctccggttgc atgatgacgc
     1141 ggtaatcggc tgcgtcgggg atcgcgccgc ccgaagtgat cgcggtcagc cgggcgcggc
     1201 gcgtggcgcg cagccggcca tgcacgccgt cgcggtggag cagcgcccgc cggccgccgg
     1261 tgtgcagctc gacggcggcg tcgaattccg cgcgcgtcaa ctcgcggtag ggccacgcgc
     1321 gccgcagccg ctcgaacagc tcggtcgcgt tccacgcttc cggcacgcac gccgccacga
     1381 tctgctgcgc caggatgtcg agcgggcggc gcggctgcgg cgtgcggtcc agctcgcccc
     1441 gtcggatcgc gctgagcagg gcgaccgcct cgaccagctc gtcgcgcgtc agcggattca
     1501 gtcgcccctt cggcgtgcgc cccaggccgt gccccgaacg gccgacgcgc tggagcagcg
     1561 tcgcgatcga ccgggttgcg ccgacctgaa tcaccaggtc gacttcgccg atgtcgatcc
     1621 ccagctccag cgaggcggtc gccacgagcg cgcgcaactg gccatgcttc aggcgctgct
     1681 cggccgcgag ccgccgctcg cgcgagaggc tgccgtgatg gcaggcgacc tgctccggcc
     1741 caaccagccg gctcagttgc gcagcgacgc gctcggccat cttgcgcgtg ttgacgaaga
     1801 cgagcgttgt gcggtgcgcc tcgatcagct ccgccatccg cgtgtagatt tcctgccacg
     1861 tttcgtgcga gcagaccgtt tccagcggcg aggagggcag ctcgatgccg agatccagcg
     1921 cgcggagggc gccctgatcc accagcgtgc aatcgcggtg cgtcccgacc aggaaggcgc
     1981 cgaccgccgc cagcggtttc tgcgtggccg acagcccgat ccgctgcggc ggccgttcgg
     2041 tcagcgcctc gagccgttcg agcgaaagcg ccaggtgtgc gccgcgcttg tcgccgagca
     2101 cggcatggat ttcgtcgacg atcaccgtcc gcacgtcgcg caacagggcg cgtccgccgt
     2161 cgctcgtcag cagcaggtag agcgattcgg gcgtcgtgac caggatgtgc ggcgggcggc
     2221 gccccatcgc cgcgcgctcg tgcgccggcg tatcccccgt gcgcaccagc acgcggatgg
     2281 ccgggagggc cggatccagt tcgcgcagct cggcgagcgg cgcctgcagg ttcttctgca
     2341 catcgttgcc gagcgccttg agcggcgaaa catagagcac gcgcgtcttg tcggggagcg
     2401 gttcgggcgc gcgcaggagc ccgtcgagcg ctgtcaggaa ggcggcgagc gtcttgcccg
     2461 agccggtggg ggcggcgatc agcgtatggg cgccgccgcg gatcgccggc cagcccgcgc
     2521 gctgcggcgc gctcggctcg cccagccggt cgcgaaacca ccgctggatc gtgggatgaa
     2581 acgttgttaa cggcatgggc ggaggcgtcg gccggcggct ggaggatcgt ccggacgcgg
     2641 cgcgcccggt gcatcatccg attttacccg ttcacgaacg aaagggccac atgcgacgtc
     2701 cgcgccaacc gcgctcgtca gcccagccgg atttccgatg agtcgcccac attcaactgg
     2761 cgatacgttc cctgcacctt ggcatggtcg gcgatgatcg aggcatcgag cagcatgttc
     2821 tccaccaggc aatggctcga cacgatcgag ttcttgatca cgctgttgcg aatcgtggag
     2881 ccctcggcga ccgtcacgta gggcccgacg atcgagtttt ccagcaccgc atccttgccg
     2941 atcgaaaccg gatgcatgat cagcgagccg tttcgttcct cgggctggcc gttcgtcgcc
     3001 gtcgtgttct tgctcagcag gatctggttg gtggagagga gggtttcggg cttcccgcaa
     3061 tcgtaccacc cctcgatgac gaacggcttc atgcgggcgc cctgcttgag catcagcgcc
     3121 agggcgtcgg tgagctgaat ctcgcccttg gtggtcacgc ccttttcgac gacttcatcg
     3181 aggcacttgt agagcatgcg cccatcggtg aagtagtaga tgccgacgat ggccaggttg
     3241 gtcgggggat gctcgggctt ttcgaccatg ctgacgatct ggtcgccctc cagctcgacg
     3301 atgccgaacc gccggccgtc ctccacttcc ttgacgccga tgtagttgct gtcggagccg
     3361 atgatcgcct tgaaatcggc gtcgaagatc gtgtcgccca ggatgaccag cagcggcgac
     3421 tggaccttgc ggtagagatc gccggtcatc cagatcgcgt gacccagccc cttgagctcc
     3481 tcctgcgtca cgaactggaa cttgaaatcg taggcccgcg tcacgtagtc gcggatggca
     3541 tcgcccaggt ggccgaccac cagggtgatc tcctcgacgt cgtatttccg caactcatcc
     3601 agaatgtggc cgatcatcgg cttgcccgcc acctgcagca tcaccttggg ggccgtgtgg
     3661 gtgtgggggc gcaggcgcgt gccgacgccg gcgacgggga taatgacgcg aagacccatg
     3721 ttttcgttgt tccttgccag gttgtatgct ctaaacggca tgcaccgccc gctccatgta
     3781 aaggccgcgg atcggcgcgg acggtctcca acctagcaga tgcacccggc aaaggaaagg
     3841 atcggcggcg gagacgaatg tagcgaggga tgaaaagcgt ccggagcgat gtaaggtcgg
     3901 cgcgatcggg cgtaatccgc cgggaccctt tggggaatgg cgaacgctga gagccggatc
     3961 ggaggagtag gggccttggg gacgttgggc gtttcccggc ttattctttg gtcagcttga
     4021 tcgttacttt ccgtaccggg tcgccttcgg gactgagcgc aacgtttttg ccccgcggga
     4081 agagatccac gccctgcggc aggtaaccct tcttctcgac aaggacctgc accatgccct
     4141 tgggcgaacc catgaagccc ttcttgaagg ggagcgtgac ggtcatcggc gtggcgccca
     4201 ccagccggcc attgaccagg acgcgcccgc cggcgggctc cgagtccagc tcgatctggc
     4261 ggtagacggc gttttcggcg ctgtcgacct tgccgacgcg atcgagcggc gcggaggcgc
     4321 cgacgctgac ccccgtgccg gggatccggt agctgacgcc ggtgttgacg ccgcaaccgg
     4381 ccgccaacag cagcaggatc ataccgcccg acaaataaag gctacggctc atcatgcgca
     4441 tctttctccc ggtccatgaa ctcagcaaaa ttcgtcgctt gtctccaatc ttaagccctg
     4501 tagaaccgcc atcaccttag cacgcggcgc gccgaaaagt ggatacgaaa cgttgatccg
     4561 cccgaccgcg ctctggccca acgagacatc gcgcgatcga tgaaagttcc aaggcgtact
     4621 gggaaaattc gccggcaggg cggaggcggc tgtcgcattg cgcccccgcg cggcggcgaa
     4681 acggcgcccg gccggcgcgg cgtgtcagtt gcgcatgctg aggatcggcg ccgggatgcg
     4741 gccgccccgg gcgatgaacg ccgcgctcga gaagcggctg acgggcatca ccggggcttc
     4801 gccgagcagg ccgccaaatt ggaccgtgtc gcccgccgcg cggccgatcg cggggatgat
     4861 gcgcaccgcc gtcgtcttgt ggttgacgac gccgatcgcc gcctcgtcgg cgatgatcgc
     4921 ggccagcgtc tcggcgctcg tgtcgccggg cacggcgatc atgtcgagcc cgaccgagca
     4981 gacgctggtc atcgcctcca ttttgtcgag cgtgagcgcg ccgacgcgaa tggcgtcgat
     5041 catgccggcg tcctcgctgg ccgggatgaa cgcgcccgac aggccgccga cgtgcgacga
     5101 agccatgatg ccggccttct tgacggcgtc ggtcagcagc gcgagggcgg cggtcgtccc
     5161 cggcgcgccg cagcgctcca gccccagttc ctccaggatg tgcgcgaccg aatcgccgac
     5221 ggtgggcgtg ggcgccagcg agacgtcgac gatgccgaac gggatgccga ggcggcgcga
     5281 cgcctcgcgc gccaccaact ggccgacgcg ggtgatcttg aacgccacct gtttgatcgc
     5341 ctcggcggcg acatcgaagc tctcgccgcg gaccttttcc agcgccgcct tgaccgcgcc
     5401 ggggccgctg acgccgacgt tgatcgccag ctccggttcg cccggcccgc agaacgcccc
     5461 ggccatgaac gggttgtcct cgatcgcatt ggcgaagacg accagcctgg cgcagccgat
     5521 cgcgtcgcgc tcgcgcgtcc gctcggccgt cgcgcggatg atccgcccca tgtgcgccac
     5581 ggcgtccatg ttgatgccgg cgcgggtgct ggccacgttg accgaggcgc agacgcgttc
     5641 ggtcgcggcg agcgccgccg ggatcgagtc gatcagccgc cggtcgccgg tcgtgtagcc
     5701 cttctgcacg agggccgaaa atccgccgat gaaattgacg ccgacgaccg cggcggcgcg
     5761 gtcgagcgtc tcggcgaacg ggacgtaatc ttcgtcgggg caggcggccg cgatcatcgc
     5821 gatcggggtg acggcgatgc gcttgttgat gaccgggatg ccgaactcca tggcgatctg
     5881 ttcgccaacg gcgacgaggt tctcgcccgc gcgcgtgatc ttgtcgtaga tccggcggcg
     5941 ggcgcgcgcg ccgtcgctgt cggcgcagtc gcgcaacgag atgcccagcg tgatcgtgcg
     6001 cacgtcgagg tgctcgacgg tgatcatccg gatggtttca aggatttcgt acggttcgag
     6061 catctcgccc ccgctgttca atcgctatcg ctctcgggag cgccatccga cagcggtccc
     6121 ggtcccgatt tcatggctca gacgtggtgc atcgcctgga acgtttcctc gcgctggatg
     6181 cggacatcca cgccgagttg ctcgccggtc gcgttcaggg ccgcgaccag ttcggggaag
     6241 ctcagcgaca tcgcggccag gtccaccagc atgatcatcg taaagtattc ctgcatgacc
     6301 gtctggcgga tatcgaggat gttgacctga tggcgcgaga gcgtcgtgct gacggcggcg
     6361 atgatgccga cgcggtcgtg gccgatgacg gtgacaatgg cgcgcaaggg acgagcctcc
     6421 cgatcgggcg tctggccgcc agggatgccg gccggcgtgg gaccggcgcc ctcgccggcc
     6481 aatgaagcag tccggcatgc ggcaaaccgg cagcatatca cacgccccgc ccgaacgcga
     6541 cgccccggcg ccgttttttg gcggtcggtt caggacgggt agaatgatgg cgggttaata
     6601 gccgtggttt ggaccggctc tcgattgcga tggcgatatc aattgcgata ccgataccga
     6661 aagacctggg ggagaaccgc tctttctcag aatgatgtca agatgctcta ttttggccaa
     6721 atcctggatc gggtcgaacg tggcgaacgc gtcgcggtgc tgcgaaacgg acgccatgtg
     6781 gcgacgatca tccccgcgcg gccggcgccc gacgccgtca agcagtatct gacagggttc
     6841 atgctcggcg cgaacgactc cgggcgatgg ggcgaggtct gacgctgaac gatctcctgg
     6901 aagcgcgaga tgaggggcgt cggtgagcga ttgtcggcgt gacggggcta tcatccggcg
     6961 gcgcgcagca aacactgttc tataccaact cgatttcgtg ccagaccagt tcgatctcgc
     7021 cgtccttggc gagggtgtcg gcggcggcgt cgaggatccg gttggccagg tcgcgcgagg
     7081 gcgacagcgt gacggcggcc aggccggcgc ggtcccaggt gttctggtcg ccgacctcgg
     7141 ccacgacgac cggatgcttc ttgcgcagat ggttcatgac cttcgccagc cggccgcgtt
     7201 tctccttgag cgagtggcag cacggcaggt gcaggtcgat gtgcaggatg ccgacggtga
     7261 agggatgccc gatggacatc tcgaagtcgt aactgggtcg ggtggccacg gtgcacgtct
     7321 cgtttcgtcg agggatgggt cgcagggggg aacgggtcgc gtgcagggag tgagggaagg
     7381 ccgccgcacc tccagggggg atttgaggtc gattcggggg ctgtccgccc cgcccttgat
     7441 gaaaccacaa ttgactcggc ggcacaaggc cgcccgaggc cggcgaacag gccgcgcagc
     7501 cggcccctgc ccctgacggg cctcattttt ttgcgttgga gtcatgaaga acgtaactct
     7561 cttgcaggaa atgcagccct tgacgggacg ccggcgagct cggttgatcg ggtccgctca
     7621 gccggccgcg gcggggacgc cggcggcggc gacttgccgg ctcgcctcgc ggcagcgggc
     7681 cacctcgccg acgatgatcg ccgccggcgc ctcgaccccc gccggcgtca gcccggcggc
     7741 caggtcgccc agcgcggcga cgaagacggc ttcgtcggcc agcgtcgcgc ggcagacgag
     7801 cgccacgggc tggccggccg gccggccggc ggcgaccagc gccgccgcga tcccggtcag
     7861 atgctccacg cccatgtaga tcacaatcgt gtcgacggcg cgggccagcg ccgcccagtc
     7921 gacgccgggg tcgccgcggt gcgccgcgcc gcagccggtg acgaacgcca cgctggacgc
     7981 cagttcgcgg tgcgtgagcg gcagccgggc cgccgccgcc gcccccagcg ccgccgtcac
     8041 gccggggatg acccgcgccg ggatccccgc gcgcgccgcc acctccagtt cctcgccgcc
     8101 gcggccgaac agcatcggat cgcccccctt caggcggacc acgtggcggc cggcgcggcc
     8161 gtgctcgacg atcaggtcat ggatccggtc ctgcgtcggc tgatgcggct cgcccggccg
     8221 cttgccgagg tagacccgca cggccgaggc cggcgcgtgg cgcagcagct cggggtgcac
     8281 cagccggtcg tagatgacga cgtcggccgc gcgcagggcg tcgcgccccg ccacggtgat
     8341 caggtcgggg tcgcccggcc cggcgccgac gagcgtcagc cgtccgccgg cgcggccgtt
     8401 cgccggcggc tcggcggccc cggccggctg ccccgcgagg ccgcgcgccg cnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgcgccgc cgccgtcagc tcgtcggcca
     8581 gtgcctgcat ccgggccagc tcatcggcca gccggtccgg ccggcgcgcc cgggcggcca
     8641 tcgccgagag cgcctcggcc ccggcgcgga cggccccggg gcagatcagc tcgcgccgca
     8701 gcgtccggcg cagctcgcgc aggcgcgtca gcgcggcgcc ataatcctcc ggaaactcgg
     8761 cctccagttc ctcgcgcagc atgcgcgcca gcgccgggcc ggcccccgcc gtcgagacgg
     8821 agaccgccag ctcgccccgg cgcaggatcg acggaaagat gaaatcgcaa tgggtcgggt
     8881 cgtcgacaac gttgaccagc acgcgccggg cgcgcgccgc cgcgtagacc cgcaggttga
     8941 gcgcgcggtc gtcggtcgcg gcgatcgcca gcagcgcccc gtccaggtcc tcctcgcgga
     9001 acggccgcgg gtggcagcgc gcgccgggca gcgcactgat cgccggcaga atccgcggcg
     9061 cgacgacgcg cagcgcggcc cccgccgccg ccagcacccg cgccttctgc agcgcgatcc
     9121 ggcccccgcc gacaaccacg acgtgcgcgc cctcaagctg gacgttgatc ggcaggtagg
     9181 gcatcgtcag atcacgtagt tcggcacgaa ccgccgcgtg ctcgcctcgt attgcgccag
     9241 caggtcggcc agcgacaggc cgtcgaagac ttggaacacc gccgcgcgcg cctggtccca
     9301 gatcggcccg aacaggtcaa cctcgccggg cggatcggac tgttcgaggc gcgcctgctc
     9361 gtcctcgatc tgtgggccgt cgatgaaccg cacgatgtcg cccaccgtga tctcctcggg
     9421 ccggcgggcc agcaggtagc ccccctcggt gccgcggcgc gattcgacga agcccccctg
     9481 cttgagctgg ctgaggatca cttccaggaa ccggaacggg atgcgctgac gctcggccac
     9541 cagcgcaatc ttgaccggtt tgtggttgcc ctggagcgcc agctcgaaca gcgcgcgcag
     9601 ggcatactgg catttctggg tcacaagcat attagggtca cagcgcgcgc gggcggcctt
     9661 cccgagtatt ccgatcaaca tagtaaatta acgcgccgcc cgctgccaag gggctttttc
     9721 cgcccagtgt gctcgttgat cggaatgaag gccatgaccg gctgcgaggc cggggggctt
     9781 atcgcaatgc gcagccgtcg cggcacgagc aggcgtgggc ccaaacggcc cggtaggcag
     9841 catcccattt agggatgcca gagcgtagcc ggtggtcgag cgtagcgaca ccaccggtca
     9901 cccggccgtc acctcgcccg catcccgggg agggatgcca gagggcagcc ggtgaatgca
     9961 gatacggatc aagtgaatca gcgcctgatg aaggccggcc gaaacctgta cgccaaccag
    10021 cccgtcgctc actcgtacca tgcctgattc tgcgccatcg gaacgatcac agcggagcaa
    10081 gccgcttccc cgcacagacc tgtgaacggt gatattcgag ataacttgga tgcggccgat
    10141 gcgcaggatt ttcaggcatt caaatacgtc cactaggggt taatccagtc accctccgcc
    10201 tgtttagctt ggttcaggta catcttagga aaatgatatc gttcctctgg cagatcatca
    10261 taattggaag aaattttggc cgtgaacacc gcgttcgcca ttgtgctgtt cccaacttgg
    10321 aagcctattc attgatgctt aatcaacagc ccacaatttt aaacgaaact cacaggagta
    10381 agtccagact aagcagaaga cttgatccat tcatccgtgc actttcctac gatgatacgc
    10441 gatggccgct gcatccggca ggaaccggtc gggcaggcgg agttttttgc cggcgaggcc
    10501 gagcagatgg tcacggtgca gctcacatag ttcgccctcg gcttccctca gccggtccga
    10561 gacgatgatg cgcagctcgt cgtcgaacga gatcaggccc cggtcgaagg cccggtcgtg
    10621 cagcacgttg aggcagatgc cgttgcgcgg gtcggcgcgg cgcttctcgt cggcgtgcca
    10681 ggggatgatg tggctcgcat tgagcaggtc ggggatcgcc agattcgtca gcgcgcagcg
    10741 gccgccgtag gccgccagca ccgccgcccg gaaaaagcgc tgcacccgac gcacccgcac
    10801 gagcgcttcc gcctcggtcg ggccgaccgg cggcggcggt ttgctcaccc gctcgcgcgg
    10861 cttcgtcggc cgcccccgcg gcgcggattg acccgccggg ccgagctccg gcacggtcgt
    10921 ttcgtcttcc aaaccggtca gctcggcgaa ggccgattcg gccgccagcg ccaccgccgc
    10981 cgagtccgcc gcgaactcct cccacaacgc ccggtcggcg cggctggcgt tgcccaatcc
    11041 cgagatccgc cgctcgcgca attgcgggtc gaggctggcg aaattgcacg ccttcatcgc
    11101 gaccgcgtcg ggcgtccggc cgatgcgttg cgccagcgcc acgatttccg gattgcgccg
    11161 gtgcaaccgg ccgaacggcg tccggcagta gagccggaag acgaccagca gttccgcgcg
    11221 cgaccacctg cccgcttggc tcgtcatggg ggaccccttc ccgccgcctg aatgaacctg
    11281 gcaggatctg gataccgcgc gggcgcgaca aatgcaaccg ggacgatcac gatgacgtga
    11341 tcgagcggac gagggacgaa ccgaggggtt acaggggggg gagggggagg ctctgggtgg
    11401 tggagcggat gcgtttgaag tcatcgcgga cggcgcgcca ctccagatgc gtcagcgaca
    11461 cgcggatgct ggtcgtttcg gcctcggtga tgatgaacag cttgccgggc acctcggcca
    11521 gggccggggc gaccggcgcg atcggctgcc aggcggcgac gagcaactgc tgcttctgct
    11581 gtgccagatc ggtcagcgcc tgagcctgat cgatcgaccc gctgaccagg ccgacctcgg
    11641 ccttgaggtt gctggactca aaatcgaggt ggaagaaata ggcctgggcg cggccgagca
    11701 gttcgcgcac ggcggcgaac ttcaggttgt gcgtccggcc ggtgccccac gccagcgcgc
    11761 cgatgttggg ccgctgcagc agggcaagcc actcggcgtc cttctgcagg ccgtcgacct
    11821 cgcccgccat gacggccagc gtgtcgttga acaggccgcg gttcagcccg aagaccatca
    11881 gcgtgggggg cggcggcgcg ttgtcgccgg cggcgggcag cgtcgggaag gcgaagctca
    11941 gttcgtggac gacgctttcg cgatactggc tctggccgct ggtctggaag acgggccagc
    12001 cgtcgccgta ggtgctctgg cggaaagggg gcgggtcgat caggaattcc tggccgagcc
    12061 agaggcgcag gcgctcgagg gtttcggcgg ggcgctcgaa ccgcccgcgc gcgatgaaga
    12121 cggcgttgcg gaagggctgg tcggcggtga cctcgccgcg gatgccgacg taaagttcgt
    12181 cgatttcctg ccagggctgg tactggacct gttcggtgaa gccctggatg acgaggcccc
    12241 agcgggggct ctggcgggcc tcgcgcagcg tctcgcgata ccagtcgcgg ccgcgcaggc
    12301 ggttgacgtc gagccgcagc agattatcca ggccgccggg aaacccgagg tagggggtct
    12361 gaaacactgt ttcgagatag ggccgctgcc ggcatccggg ggccagcagc agcagcgcga
    12421 gcacagccag cgaaaggcgg gggagaggga gcttcatagc tggagtcgta cccttcagca
    12481 aggtggggga ggaaggcgaa cggcaagcgg aacatccggg tctcacggga gtcgcggtcg
    12541 gcgcgacggg gttcggattg gggctttcgg tggttgcggg cgaagcggcg aaggggatcg
    12601 gccctcgccg ccgcggattc gccctcgccg gtgtcggcca ccgcgacatt gagagaattc
    12661 agcttaagat caatcctatg ccatgggcaa ggcttcgcct agcgtgaaat gcggaagcgg
    12721 ggggatttga cggccagtcc cgccagggac ggcggacgtc agcccgctac tgcagtggcg
    12781 ggaaccggga tcgcctgagc gaccgagtcc catcgggacg gcggaaaccg gggggagaga
    12841 agggtggcat ttcgagagcc gagggcgagg gacgagagcc gggagccgaa ttttgggacg
    12901 ggcctctgcg ggcgatcacg aagtcgaggg gcgagggacg aggttcgctg gaggggtcag
    12961 atgcggtcgc ggatgaggga caggacgcgt tcgacggtgg agcgggagtg atcgagcgtg
    13021 tcggcgaggc ggcccggctc ggtcgggttc tccagctcgg cgccgaggcg ggcgcgcaga
    13081 tcgaccagtt cggtctcgat ctggcgcagc gccagcaggt cgtcgcggcg tttctggcgc
    13141 gcggcgtcga gttcgcgccg gtagatgtgc gaacggtcgg cgcccttgag cagaaggtat
    13201 tcgacgagca tgatgacgac aatgaacgcg gcataggggc tgtagagcca gttgaggatg
    13261 gcgcgcagat tgcgcaggtt gccggtgatg acgatcaggg cgaggatgcc gatcgtcatc
    13321 aggatgatga tctcgcccgg cccgattcgc agccgctgcc gccagcccat gcgccgcctc
    13381 ccttacccac gaaatagggc cactatagcc gaagcgcggg cgggatgcga cagccgagag
    13441 acggggagtg ggggcttcga ggtggggtgg ggcgagggat gtttggatca cgaaacgcac
    13501 gcatcgcgag ccgaggacga gggacgattt gacgtggggc gtgggcgtcg catttggcgg
    13561 ggcggtctgg tagggtgaac ggggagtgga accatgaagc aagtcagcgt cgaactgatc
    13621 cgcaacgaga ccttgattcc cggcacggtg ctgcatcact accgcgatgc ggagatcgcg
    13681 gcgacggcgc ggccggggca attcgtcaat atccggccgg gctacgggag cgatccgctg
    13741 ctgcgccggc cgttcagcat ctgcacggtg gaccggtcgg ccgggacgtt cacggtgctg
    13801 atcaaggaag tgggggcggg gacgcggatg ctgctggcgc tgcggtctgg cgcgcgggtg
    13861 gacatgctgg cgccgctggg gcggagtttc gcctgggacg gggcgcggcg gctgctgctg
    13921 gtggcgggag gggtcggcgt ggcgccgctg ctgttcgtcg ccaacgaggc gcgcgcgcgg
    13981 gagaccggga ccgagccggc gccggaagtc atcttctgct acggggcgcg cacggcgggc
    14041 gatttcgtgc tgctggacca gatccggccg gtggcgagcc ggctggtgct gacgaccaac
    14101 gacgggtcgc tgggcgaggc ggggttcgtg acggcggcgg cggagcgctt tttcacgccg
    14161 gagacgacga tcctgacctg cgggccgaac ccgatgatgg acgatctgct gcggcggatg
    14221 cgcacggccg ggctggaggg gcaggtcagc ctggaaaacc agatgggctg cgggatcggc
    14281 gcgtgccagg ggtgcgtggt gccgacgcgg cggggctacg tgcggatctg ctgcgaagga
    14341 ccggtgctgc ggaccgacga gctggatgag atccggtttt gaatagggga tcacgaaaga
    14401 cacgaatggg cacgaaatgg atatgcgctg ttcgtggacc cggaggttca gaacgaggag
    14461 ccaaattgat ttcgagagcc gaggacgagg aacgagagcc gggagccgaa ttctgggcgg
    14521 gccgttgggg gcgattccga agacgcggga cgatgtgaag gggaactccc ccgtcccggc
    14581 ggggaggggc gctggtccgg gacgggggag ccgcggggtg accgcggttg ggggagaaag
    14641 ccacggaagg gaggtgagag gacacgcccc ttcggggaag gattcaccgc tgcttccctt
    14701 aaactataag tgaggatcgg cggcgtgtca acccctgtgc gtaaaaaaaa cgggggcggc
    14761 gcgagagcgg agttttgggg ttgggacctt cgggagcgag gacgatttca gggtgaggga
    14821 cgaattttga tgtgggggga tgagccccca tccaccggtt caggcgtcag ttgcgccggc
    14881 ccatcacgag catcaggaaa tacagcatct gaaggatggc ggtgacggtg gcggcgacgt
    14941 aggtcatcgc ggcggcgttc aggacgctgg cgaccgcgcc ggcctcgcgc ccgggctgga
    15001 cgatgccgag ctggacgacc atctgcttcg cccgcgccga ggcattgaac tcgaccggca
    15061 ggttgacgag ctggaacacg acgaccgtgc cgaacagcag gacgccgccc atcgcgatgt
    15121 agaagcccag cgcgctctgc gtcagggtgc tgatcccgag gccaatcatg acgaggatca
    15181 tgccgaaatt cgagccgaag ccggcggtcg gcacgatggc attgcgcagg acgaggggct
    15241 tgtacgcctg ggcgtcctgg agcgcgtggc cgacctcgtg gcaggcgacg ccgacggcgg
    15301 ccaggctggc ctgatcgtgg acggccgggc tgaggcgcag gaccttgtgg cgcggatcgt
    15361 aatgatccga cagatggccc gagacgcgtt cgatcgagac gtggcggtcc atgccggcgg
    15421 cgcgcagcat tgccctcgcg gcctgggcgc cggtcaggcc ggagctggcg cggaccttac
    15481 tgtatttgga gaacgccgac ttgacccaca ggctggcgcc gccggccagg atcagcgtcg
    15541 gcaggaagac gaagaggaga tatcccggat cgaagaacat gttcgagctc gctttcggtc
    15601 aatcgtgatg ccaggcgcga accgcgaagc ggaagcaggg ctcgctgcct cgcgcctctc
    15661 gaatgcaagg catatgccgg atgggcgggg gtggggggaa tcccccccgg cggcggaaat
    15721 catgctaaat catggcgcga gagcttggcc agcgaaagcg gccgcgaagc gccgcggcgt
    15781 tggggagcgg cattctgacg cacagtgttg cataccgcac catgttcgga ggcggcgggc
    15841 gcgagagagc aggcgattcg cggcgcggcg gatttgagtg cggcgcggga tgcgggaatt
    15901 ctgccgtcac atcgaaaatg aaatcgattt tcgggtgcgg aagtcatatc cttcctgcca
    15961 gttgatcgct gctccattgt cccgcacaac tcccaatcgt cgcgtcagga gttgtcctgg
    16021 tttatgccag tccgttg
//
LOCUS       DAOPCO010000061        15960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3731_length_15960_cov_4.844892, whole genome shotgun sequence.
ACCESSION   DAOPCO010000061 DAOPCO010000000
VERSION     DAOPCO010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3731_length_15960_cov_4.844892"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PLS90_06420"
     CDS             complement(<1..1056)
                     /locus_tag="PLS90_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPK02074.1"
                     /translation="MTEFDVIAAGHLCLDITPAFPDYLKPSITEIFKPGKLLNVGTAK
                     INLGGPVANTGLAMRKFGLRLAIVAGVGDDLFGQAAVTLVRQLGAESAIHVSPGETTS
                     YTVVMSPPSVDRMYLHCPGKNDTFRSADIDSAILSRARLFHLGYPPLMRALYADDGRE
                     LVAIFRRAKAAGLTTSLDMSLPDPDSPSGQVNWRLILERLLPFVDIFLPSIEELFFML
                     DRDGYGNLRARFPDDDLVDHVPVEVYRRLGRQAIAMGCGICLVKASHRGNYLCSADSD
                     RWERFGAAARPQPDAWRRRELWAPAYHVPRIASAAGSGDSSIAGFLTAFLRGLPPARA
                     MQLANAAGAFNLRELDTL"
     gene            1766..2431
                     /locus_tag="PLS90_06425"
     CDS             1766..2431
                     /locus_tag="PLS90_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02075.1"
                     /translation="MESLISRKAAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKIS
                     RAKADMRSIAVGVESFRLDNNRWPTNTNATFWGSYLLWTEYINELTTPIAYLSSNQFV
                     DAFNPARLDAFSQPFIPVGSIGSYFYADYSVDKNPYSWGYGRKYNQGLNYPGFDAFGL
                     FSWGPDRNWDRAPNMIEQLSPPPAKLVQWAVIDLIYDPTNGTISDGDIGRFGGAAPAN
                     TTP"
     gene            2501..3862
                     /locus_tag="PLS90_06430"
     CDS             2501..3862
                     /locus_tag="PLS90_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02076.1"
                     /translation="MMFKNGYYSWLIAAMACVLTIHAEAAVSVNGVSGEDDDGMPPFE
                     SVQQAVIYAIDNGENEVTLDANDISGWSFRLLNNEAQLNPQWPDGTFTIRAGDGFSPV
                     VTGGNNYLCPHDFPNTTIVLEGFTIDSTTFGLITSGNVTLRDMEIVVTSGGGHACLLS
                     NVNGTNIHFQPGTKLKVENTVLRRVNAAGSIMVGGHGHQSYEFRNCQFISDSSGPSVL
                     TFAGDETVGTAEDVDLTVVGCTFDVATGALNYSAADSSTAGLEFVNNVVLSAGEHSTA
                     APVVALGNNHAASRIVHSTFRVQGTANVTSLVNLAATMTTTPINLSNSLFDAPIAGIA
                     GVSVGDAGQAGALTGDANAYNMETDHAYQVAGADQGNGFEAANRYLQNATNGVYISDV
                     NGRITANATLVVGRAVAVTPAVTTDIDGESRPQGPASDIGADEVAEAGGGVTGAARWT
                     TFE"
     gene            4004..5377
                     /locus_tag="PLS90_06435"
     CDS             4004..5377
                     /locus_tag="PLS90_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02077.1"
                     /translation="MIKLQKVLCLAAMGLFGLAHAAVADVSVNGVAGEDDDGAPPFET
                     IARAVLYATTSTQTVVTIDTDGPLPGWGQGLDDDPEEIGGYPAGTFTIKAGDGFSPVV
                     EGNNNYIRGTRNLQTEFILEGFTLHGPSANTLMCSGNVTLRNMTISSGSSNGAHQAIF
                     AVFQATNILFEPGTQLLIEDCNISGPRAYLGGVGFQTTIIRNSVLSSTIAAPPIQVQQ
                     RDIATGMNLIMEGCTVNHRGSGLIFDSQAGASLNSGSVSLVNNVFLGGTLAHGNNAVI
                     GILNKAVDVSVRHCTLRANDAGMAIANGVLLGASVTTTTVEIANTLFDLPHADKIGVS
                     IGDAGQLANVSGDANAYNLGEANSLVVAGDPATDYFEATSRYVQDNTTGTRFVTRATD
                     GLLTLDFDPNSEVYHSALVSDRAVALTPPVADDIEGESRPQGGAADIGADEWGYVPAE
                     LSTFEVQ"
     gene            5487..6959
                     /locus_tag="PLS90_06440"
     CDS             5487..6959
                     /locus_tag="PLS90_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPK02078.1"
                     /translation="MIARREFLKATLAASGGLALAPLSLSGGASRAAAPAGARPNIIF
                     YFCDDLGWGDLGCQGNPVIQTPRLDQFAAESCRFTDFYATAPVCGPSRAGAMTGRIQN
                     RFGMEWLCNHTFQPTMPMYHHVPLEEPMLPRLLQQAGYRTGHIGKWHLSLLEFEGEPF
                     PPEYGFDYYFIVEGVGGKSFYANPTDWYRNGETIPGRLADWAETMYVDEAIRFIEEKP
                     GVPFFLNLWTFTPHEPLASPQSYQELYPDRMPQEKVYFGAVTQMDAQFGRLMDYLQRA
                     GLAENTIVIFSSDNGPEHYAALDNRICRGSSGPFRAGKRRLYEGGIRVPGIIRWPGVT
                     TAGSVSHEPASTVDLLPTLCKAAGAALPTEITFDGADLRPLLAGDSAVERPHPLFWRM
                     FDADRPYFRHSAYPSPPLAMRRGRWKILSDFEYAKPELYSLDIDIGEQWNVAERYPEV
                     TAELMDELKRIHEDVIQPYPSERYLNPHVVETVPRVKPSA"
     gene            7036..8118
                     /locus_tag="PLS90_06445"
     CDS             7036..8118
                     /locus_tag="PLS90_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK02079.1"
                     /translation="MLAALYRETPDRLPATVHMWHDYHLRHFMDGMSAEEAFAACGLD
                     AAITFFPFIEEPASPDWKTECVPSQNEKGEQVQKCRIDTPEGTLHYEMASNEITTWIN
                     RHPIQHDEDIYLIEKYQPIPRLLRNELARAYDALGDAGIMRGLLPAFNFQSGIWQEAV
                     EWVGTEKLIMAVYEKPDWVHAFLRILLEKRLAFIEREMRGAKFDLIETGGGAASDTVI
                     SPDVFRDFALPYDRQMHAALHEAGHRVVYHTCGGMTNLLNLIPENGCDASETLTPPGM
                     GGNLTDPHRVKASLGAKVCLIGGMDQNNVLKQGDAMVIQAEVRRLFETYGRHGGYIMA
                     TCDQFFDVPRESLRAYADAARQCMYA"
     gene            8151..9761
                     /locus_tag="PLS90_06450"
     CDS             8151..9761
                     /locus_tag="PLS90_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPK02080.1"
                     /translation="MELRISHLDLVIIIAYLAGIVLVGCVAGWRQRRGMKVDSQGYFL
                     AGNSLTWPIIGMALFSTNISTAHIVSMAQEGYTNGMAWGNFEWMASFTLIILALFFAP
                     FYIRSRVPTLPDFLERRYNRHCRDILAVISIISAVFIHIGVTLYSGAVLLKGLFGLDI
                     MFSIIVISVLTGLYTIIGGLLAVVLTESIQTVILLVGAVCVTAAGLWRVGGWEGLTSH
                     VTAVDMTVIRGGDDPSGMAWWGVFLGYPVIGIWYWCADQTIVQRVLGARDENHARSGA
                     LFAGFIKILPVFLFVFPGLICKALVSQGEFGPGPEDPAETFPYLVNHLLPVGLTGIVA
                     AGLLAALMSTVSGALNSIALLVSYDIVRRWRPQTSEPALVTIGRVVTFVAMVCAILWS
                     PLLGTKFNTLAQAAATMICCIAPPITTIFLWGVFWRKASAIAAGCTMWIGGLLGLIVF
                     LMLFFEVEWWPIGFMMGAFYLFVVCSILLVVLSLIFPQAHTTESKSLVWSSFWEPLRS
                     PGWPGLANYRVVAGILCVVMVGLYIYFS"
     gene            9778..10218
                     /locus_tag="PLS90_06455"
     CDS             9778..10218
                     /locus_tag="PLS90_06455"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: protein motif:HMM:NF017174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HPK02081.1"
                     /translation="MGLKSGVGLAWMMLTVLLGGTGCQRAQVMKGEMMPQRYCMILGL
                     QEGALDEYAEYHRDVWPDVLDALRSANLTNYSIYVFHADDGLPYLVGYFEYVGSDFES
                     DMARIGELPVIKRWNDTMSKLQRPIDNRKDGEWWAAAEELFHME"
     gene            10215..13136
                     /locus_tag="PLS90_06460"
     CDS             10215..13136
                     /locus_tag="PLS90_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPK02082.1"
                     /translation="MTATPCGTSRKVGKRTDEMKHSETTPSLETTVHPGADGPPFSHA
                     TSLRQLGMIVSHTHWDREWRYPIWETRFHLQQMMDELIALLEKGVYSNFLLDGQTVIV
                     DDYLMFRPDMARRIEALVRDGKLSIGPWYTLPDLSVIDAECVVRNLLWGRRRADALGG
                     CMKIGYTTFGWGLPAQFPQILAGFDIRFITISKYLDPRRLEHNEFVWRAPDGTEALAT
                     IIGEHARAGFFFSCYQDILVGRRYLGEDWKYDFSAGRLPFHRADPPAAHRDYCLLEPP
                     RGLHPELANRSTVDQCMRNTDGSLMRHTRLMMNGTDSIFPSSIFPKLVETVNQVDPDR
                     QFVQASLAEYVDVLQAELHQEHLQVVQGELRDGPAAKVSGNALSTRLYIKQMNRRAEN
                     ALLRNAEPLAVASWLSGQEYPEEFLNKAWNFMLKAHCHDSINGVTLDKTARDTVGRLD
                     QVLDLANGTINSALRYIVRQMNLVIQDERETLLVIFNCLPRQRREVAVAYVDFPVDWN
                     VDLFEFVNPEGERLAVQTLNKSIINVSAWSDLHRAFPFHVERHEVAFDTGPLAACGYT
                     VLRAVPTRFKDRTKVIWHDPVPVRNQIAPNSRTLENECVRIQINENGTFDLHDKVNQT
                     VYRGLNYFEDIGDAGDYWVRTEPCRNRTVNSIGSRARIELIENGELRASIAVTVSLHV
                     PDRDAQDDLSRELDLHYRVTLQAGRPEVEVEVWFENRIPNHRLQVMFPTGLSDAVRIV
                     AETHFATNRKPFLIKRLEDDAFYLDMQSQPMGRFLDIADRERGLAIISNSLTEYQGCN
                     DQEKTVGLTLLRSVKNEMCAEFRTVAENADENGGQCFGRHRASYTLLAHGADATQALI
                     AQLADRHGTPLVMVQTMANTGPLPGTRFSWFELIGERVRLSTIKRAHDRPSVIVRLYN
                     PTDAEACAELRSAMRLTASWITDLNEERKRELEILNDRQVRLTLEHHKIATVELVVDR
                     D"
     gene            13180..13875
                     /locus_tag="PLS90_06465"
     CDS             13180..13875
                     /locus_tag="PLS90_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HPK02083.1"
                     /translation="MKRSTINRYIRATEAFFAERQFHLPDFGHWTPEDWRQKGPETRE
                     IAECQLGWDVTDFGLGDFEKTGLVAFTVRNGLNQQLSAGGKPYCEKIMRVGEAQVTPW
                     HFHWNKMEDIINRGGGNLIIAVANVSEDEGLAGTPVELSIDGVRHTVPARTEVSFAPG
                     QSLTIPPYLYHAFWAEPGSGPVLCGEVSKVNDDHHDNRFLKAIRFSEIEEDESPYRLI
                     VGDYERYYSALSS"
     gene            14698..>15960
                     /locus_tag="PLS90_06470"
     CDS             14698..>15960
                     /locus_tag="PLS90_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPK02084.1"
                     /translation="MQQPGWAMCNSWGIYTSVFNVDYPIYIENAYELLDQPAEWYLDR
                     AEDKVYLIPFGSARPGEIEVVAPILERLIRFDGTAVDPVHDIRVEGLVLCDATWDMGA
                     PAYGYPSIQATVMGAAGQENVHGTLNEKHQVPGNITLAGAQKVKFIGNTFTRLAAVAV
                     ELVSACSDCQFIGNLFTETSGGAMQLGRYNEPYLPSEETIRNTLFENNYIHDVALDYR
                     DHPAITCFFTDSTVIQHNEIERVPYSAISMGWGWGRLEEKHGQTIARNNQILNNHISN
                     HMLVMEDGSAIYCLGWQPELLIEGNHIHDTGAVISPGIYLDRGCRYMTVRNNVIYNCE
                     SNMMVKGTDHLIEYNFWQDEFSTRRPGAPSHWVYWGVATLYWKIAHNVVVMDEASMPA
                     SIIQQAGIRPQDAHLLPVRGGLDSQPPTV"
BASE COUNT         3209 a         4503 c         5114 g         3134 t
ORIGIN      
        1 cagcgtgtcc agttctcgga ggttgaacgc gccggccgcg ttggccagtt gcatggcccg
       61 cgcggggggc agtccgcgca ggaaggcggt cagaaagccc gcgatggagg aatcgccgct
      121 gccggcggcg gaggcaatac ggggaacatg ataggccggc gcccacagtt cccggcggcg
      181 ccacgcgtcg ggttgcggcc gggcggcggc gccaaaccgc tcccatcgat cggaatcggc
      241 ggagcacagg taattgcccc ggtgcgatgc cttgaccagg cagatgccgc aacccattgc
      301 gattgcctgc cgtcccaggc ggcgatagac ctcaaccggc acgtgatcga cgaggtcgtc
      361 atcgggaaac cgggcccgga ggttgccata gccgtcgcga tcgagcatga aaaacagttc
      421 ctcgatgctg ggaaggaaga tgtcgacgaa cgggagcagc cgttcgagga tgaggcgcca
      481 gttgacttga cccgaagggg aatcgggatc gggcagcgac atatccaggg acgtggtcag
      541 gccggcggcc ttggcccggc gaaagatggc gaccagctcg cggccgtcgt cggcgtagag
      601 cgcgcgcatc aggggcgggt aacccagatg gaacaggcgc gcccgggaaa gtatcgccga
      661 gtcgatatcg gcgctgcgaa aggtgtcgtt cttgccgggg cagtgcagat acatgcgatc
      721 gacgctgggg ggcgacatga cgaccgtgta ggaggtcgtt tcgccgggcg agacatggat
      781 agcggactcg gcgccaagct ggcgcacgag cgtgacggcg gcctgaccga acagatcgtc
      841 gccgacgccg gccacgatgg ccagccgcag gccaaactta cgcatggcca acccagtgtt
      901 ggcgacgggg ccccccaggt tgatcttcgc cgtgccgaca ttgaggagtt ttcccggctt
      961 gaaaatctcc gtgatcgagg gtttgaggta atccgggaag gccggcgtga tatcgagaca
     1021 gagatgaccg gcggcgatga catcgaattc ggtcatgaat cgggcctcca gtgattgaca
     1081 gcgcgatcaa aaactccgca acgcttatat gactttctcg gcggagaggg caaccgtagg
     1141 gcgtaaatag ttatacacca ttttgccgga agtcaaactc caaaatcgag ttgccgacgg
     1201 ggcgtcctta cctcgcgggg aggcgcgata cagccagatg cgggcaattt attcttaata
     1261 aacagcggag tggagcatag agaagtgcag catggggcgt tgtcaatgcg agcggatcaa
     1321 gcggtttgcg ccggccgatg atatgcatat ttttttaatt tttttcttgc gcgccttcct
     1381 gattatgtgt atctaagacg caacgacggc aaaagatcgc ctgaccccgg ctctgcttgc
     1441 atcgacttca aaggcttcct atggattcaa ggctgggggc cggcactggg gcgggacaaa
     1501 ccacttcagc tcaaccgtgc cgcgcgccct ttcggattcg gggtttgtgc agggggccgc
     1561 ttcagttatt ccatctgaag gacggggcgg ccgccgtgta tcggccgtgg gaggggtctt
     1621 tgattccccg gacggcattg aactgagagc ctgcgccgga acggcactcg gggggaaagg
     1681 aggggagcga cgaacacatt ttattggtct ggtcgggaaa gaatcgttcc tccattcatt
     1741 cacggcgttt gaaaggatac atcccatgga atctctgatt tcccgaaagg cagccttcac
     1801 gctgattgag ctgctgattg tggtcgcgat tatcgccatc ctggcggcca tcgcggtgcc
     1861 gaattttctg gaagcgcaga cgcgctcgaa gatcagtcgc gcgaaagccg acatgcggtc
     1921 gatcgccgtc ggcgtcgaat cgttccgtct cgacaacaac cgctggccga ccaacacaaa
     1981 tgccacgttc tggggctcgt atctgctgtg gacggagtac atcaatgagc tgacgacgcc
     2041 gatcgcctat ctctcctcca accagttcgt cgacgcattt aaccccgccc gactggacgc
     2101 gttctcccaa ccctttatac ccgttgggtc gatcggatcc tatttttacg cggactattc
     2161 ggtggataag aacccctata gctggggcta cggacggaag tacaaccagg ggttgaacta
     2221 cccgggattc gacgcatttg gtctgttcag ttggggtcct gaccgcaatt gggaccgtgc
     2281 gcccaacatg attgagcagt tatccccgcc gccggcaaag ctggtgcaat gggcggtgat
     2341 cgatctgatt tacgatccga ccaatgggac gatcagcgac ggcgacatcg gccggttcgg
     2401 cggcgccgcg cccgccaaca ccaccccgtg atgggggacg gcgagctgcg ggaatttgga
     2461 gtctggcggt cccctttcaa ttcgatgcac aaggagaacc atgatgttca agaacggata
     2521 ctactcatgg ctgatcgccg cgatggcgtg cgtcttgacg attcatgcgg aggccgcggt
     2581 ctcggtcaat ggcgttagtg gagaagacga cgacgggatg ccgccgttcg agtcggttca
     2641 gcaggcggtc atctatgcga tcgacaacgg cgagaacgaa gtcactctgg acgcgaacga
     2701 tatttccggt tggagctttc ggctgctgaa caatgaggct cagttgaatc cgcagtggcc
     2761 cgacggaacc tttacgattc gcgcgggcga cggcttttcc cccgtcgtca cgggcggcaa
     2821 caactacctc tgcccgcacg actttcccaa taccaccatc gtgcttgaag gattcaccat
     2881 cgattcgacc acgttcggcc tgattacgtc gggaaacgtg accctgcgcg acatggaaat
     2941 tgtggtcacg tccggcggtg gacacgcctg tctgctgtcg aatgtgaatg ggacgaacat
     3001 tcactttcaa ccaggaacca agctgaaggt ggagaacacg gttctgcgcc gagtgaacgc
     3061 cgccggctcg atcatggtcg gcggacacgg gcatcaatcg tatgagttca gaaattgcca
     3121 gttcatcagc gattcgtccg ggccaagcgt gttgacgttc gccggcgacg agaccgtcgg
     3181 caccgccgag gacgtggatc tgaccgtggt gggctgcacg tttgatgtcg cgacgggcgc
     3241 gctcaactat tccgccgcgg actcgtcgac ggcgggcctt gagttcgtga acaacgtcgt
     3301 cctgtcggcg ggcgagcatt ccaccgcggc cccggtggtg gccctcggca ataaccacgc
     3361 cgcgagccgg atcgtgcatt caacgttccg cgtgcagggg acggccaatg tcacgagcct
     3421 ggtgaatctg gccgccacga tgaccaccac gccaatcaac ctctctaact cgctgtttga
     3481 cgcgccgatc gcgggcattg ccggcgtgag cgtcggcgat gcgggccagg cgggcgcgtt
     3541 gacgggggat gccaacgctt acaacatgga gaccgaccac gcctatcagg tcgcgggcgc
     3601 tgatcagggc aacggctttg aagccgcgaa tcgatacctg caaaatgcga caaacggcgt
     3661 ctacatcagc gatgttaatg gccggatcac ggcgaacgca accctggtcg tgggccgggc
     3721 cgttgccgtg acgcccgcgg tcacgaccga catcgatggg gaatctcgcc cgcagggccc
     3781 cgcatcggac atcggggcgg acgaagttgc ggaagccggc ggcggagtga ccggcgccgc
     3841 gagatggacg acgtttgagt gagacgcttt ccaatgaaaa atcgtgctgt caatagcggg
     3901 aacacccccg gtggctgacg aagggggtgg tgaatcccag gctgggtgag aggaacccga
     3961 tcagcgcaat cacaagcctt ggaacgggga gaggagaagg aacatgatca agcttcagaa
     4021 ggtcctttgt ctggccgcaa tggggttgtt cggcctggcg catgccgccg tcgcggatgt
     4081 gtcggtgaac ggggtcgcgg gcgaggatga cgacggggcg ccgccctttg agacaattgc
     4141 gcgcgcggtg ctctacgcca ccaccagcac gcaaacggtc gtcacgattg acaccgatgg
     4201 cccgctgccc ggctgggggc aaggcttgga tgacgatccg gaggagatcg ggggttatcc
     4261 ggccggcacc ttcaccatca aagcggggga cggcttttcg ccggtcgtgg aaggcaacaa
     4321 caattatatt cggggcacac gcaatctgca gaccgaattt attctggagg ggtttaccct
     4381 ccatggcccc agcgccaaca cgctgatgtg ctccggaaat gtgacgttgc gcaacatgac
     4441 gatcagctcg ggatcaagca atggagcaca tcaagctatt tttgccgtat tccaggccac
     4501 gaatattctg tttgaacccg gcacccagtt gctgatcgag gattgcaaca tatccgggcc
     4561 gcgcgcctac cttgggggcg tcggtttcca gacaacgatt attcgcaatt ccgtcctgag
     4621 ctccacgatc gcggcgcccc cgattcaagt tcagcaacgg gatatcgcaa ccgggatgaa
     4681 cctgatcatg gagggctgca cggtcaacca tcggggaagc ggcctcattt ttgactcgca
     4741 ggccggcgcg agtctgaatt ccggttcggt cagtcttgtg aacaacgtct tcctgggcgg
     4801 cacgttggcc catggaaata atgccgtcat cggcatattg aacaaggcgg tggatgtgtc
     4861 ggtccgccat tgcaccttga gggcgaatga cgcgggcatg gcgatcgcca acggggtgct
     4921 gctcggggcg agcgtgacga cgaccaccgt cgagatcgcc aacacgctgt ttgacctgcc
     4981 gcacgccgac aagatcggcg tcagcatcgg agacgcgggc cagctggcga acgtcagcgg
     5041 ggatgcgaac gcctataatc tgggggaagc caacagcctt gtcgtcgccg gcgatcccgc
     5101 gacggattac ttcgaggcca ccagtcgcta cgttcaggac aacacgacgg gcacgcgctt
     5161 cgttactcgc gcaacggacg gcctgctgac actggacttc gatcccaaca gcgaggtgta
     5221 tcatagcgcg ctggtttccg atcgggcggt tgcgttgacg cctccggtgg ccgatgacat
     5281 cgagggcgag agtcgtccgc agggaggcgc ggcggacatc ggcgccgatg aatggggata
     5341 tgtcccggcg gaactgtcaa cgttcgaagt gcaatagcac cggcggaagc cgcggccggg
     5401 ggcgccgcgt tgggcgcgct cccggccgtc ttcgcctacg aaaccgcacg gtgaaaccac
     5461 cgactgcgca acaggcagcg tgcaagatga tagctcgacg tgagtttctc aaagccaccc
     5521 tggcggcgtc gggtggtctg gcgctggcgc ccctatcgct atccgggggg gcgagccgcg
     5581 ccgccgcgcc cgcgggcgcc cgaccgaaca tcatttttta cttctgcgac gatctgggct
     5641 ggggggatct gggctgtcag gggaatcccg tgattcagac gccccggttg gatcagtttg
     5701 ccgcggaaag ttgccggttc accgactttt atgcaacggc gcccgtctgc ggtccgtcgc
     5761 gcgccggggc gatgaccgga cggattcaga accggttcgg catggaatgg ctctgcaacc
     5821 acacgtttca gccgacgatg ccgatgtatc accacgttcc gctggaggaa ccgatgctgc
     5881 cccggctgct tcaacaggcg ggctaccgga ccggccacat cggcaaatgg cacctgagcc
     5941 tgctcgaatt cgagggcgag ccgttcccgc ccgaatacgg tttcgattac tatttcatcg
     6001 ttgaaggcgt gggcggcaag agtttctatg caaaccccac ggactggtat cgcaatggcg
     6061 aaaccatccc cggtcggctg gccgactggg ccgagacgat gtatgtggac gaggcaattc
     6121 gctttatcga ggaaaagccg ggggttccct tttttctcaa cctctggacc ttcacgccac
     6181 acgaaccgct ggcatcgcca cagtcctatc aggagctgta tcccgaccgg atgccccagg
     6241 agaaggtcta ttttggcgcc gtgacacaga tggatgcgca gttcggccgc ctgatggact
     6301 atctgcagcg agcgggtctg gcggaaaata ccatcgtcat tttctccagc gacaatggtc
     6361 cggagcacta tgccgcgctg gacaatcgta tctgtcgtgg gtcttccgga ccgtttcgcg
     6421 cgggcaaacg ccgcctgtat gaaggcggaa tccgcgtgcc gggaatcatt cgctggccgg
     6481 gcgtgacaac ggcggggtcg gtgagccatg aaccggccag caccgtggac ctgctgccga
     6541 cgctgtgtaa ggccgccggc gcggccctgc cgacggaaat caccttcgac ggcgccgacc
     6601 tccggccgct gctcgcgggg gattcggccg tggagcgacc tcacccgctc ttctggcgga
     6661 tgtttgatgc cgatcggccg tatttccgcc acagcgccta ccccagcccg ccgctggcca
     6721 tgcgtcgcgg gcgctggaaa atcctcagcg atttcgagta tgccaagccg gaactctaca
     6781 gtctggatat cgacattggc gagcagtgga acgtcgccga gcgatatccc gaagtgacgg
     6841 cggagctgat ggacgaattg aaacggatcc atgaggacgt gatccagccg tatccttcgg
     6901 aaagatacct gaacccgcac gtggtcgaga ccgtgcctcg agtcaagccg agcgcctgac
     6961 acccggcgga cttcgggacg gataatcgtc gattcaaagt cagaggacgc ttaacggatg
     7021 aactccaagg atcgaatgct ggcggcgctt taccgggaaa cgcccgaccg gctgcccgca
     7081 accgttcaca tgtggcatga ctatcatctg cggcatttca tggacgggat gagcgccgaa
     7141 gaggcctttg cggcgtgcgg actggatgcg gcgattacct tctttccgtt tatcgaagag
     7201 ccagcctcgc ctgattggaa aacggagtgt gttccgtcgc aaaacgagaa gggcgagcaa
     7261 gttcagaagt gccggatcga cacgccggaa ggcacgctgc actacgagat ggcaagcaat
     7321 gaaatcacca cgtggattaa ccgccacccg atccagcacg acgaggacat ctatctgatt
     7381 gaaaagtacc aacccatccc gcgactgctt cgcaacgaac tcgcccgtgc ctacgatgcg
     7441 ctgggcgatg cggggatcat gcggggcctg ctgccggctt tcaactttca aagcggcatc
     7501 tggcaggagg cggtggagtg ggtcggaacc gagaagttga tcatggcggt ctatgaaaag
     7561 ccggactggg tgcatgcgtt tctgcggatt ctgctggaaa agcggctcgc ctttatcgag
     7621 cgggagatga ggggcgcgaa attcgacttg attgagaccg gcggcggcgc ggcctccgac
     7681 accgtcatct cgcccgacgt gttccgggat ttcgcgctcc cttacgaccg gcagatgcac
     7741 gcggcgctgc acgaggcggg gcatcgtgtc gtgtatcaca catgcggggg aatgacgaac
     7801 ctgttgaacc tgatcccgga gaatggatgc gacgcctccg aaaccctgac gccgccgggg
     7861 atgggcggaa acctgaccga tccccatcgc gtgaaggcct cgctgggcgc gaaggtctgt
     7921 ctgatcggcg ggatggatca gaataacgtg ctcaagcagg gcgatgcgat ggtgatccag
     7981 gcggaagtcc gacgcctgtt cgaaacatac ggacgccacg ggggatatat catggcgact
     8041 tgcgatcaat ttttcgatgt accacgcgag agcctgaggg catacgccga cgccgcgcgg
     8101 caatgcatgt atgcctgagg ccacggattt cgcggtagaa agttgtctgg atggaacttc
     8161 gcatctcgca tctcgatctg gtcatcatta tcgcctacct ggctggcatc gtgctggtgg
     8221 gatgtgttgc cggctggcgg cagcggcgcg ggatgaaggt cgacagccag ggctattttc
     8281 ttgccggcaa ttcgctcacc tggccgatca tcggcatggc cttgttctcc accaacatct
     8341 ccaccgcgca tatcgtgtcg atggcgcagg agggctacac caacggtatg gcctggggca
     8401 atttcgaatg gatggcgtcc ttcacgctga tcatcctggc gctgttcttc gccccgtttt
     8461 atatccgttc gcgcgtgccg acgttgcccg attttctcga acggcgctac aaccggcact
     8521 gccgcgacat cctggccgtc atctcaatca tatcggccgt tttcattcat atcggcgtga
     8581 cgctttattc gggggcggtc ctgctcaagg ggctgttcgg gctggacatc atgttcagca
     8641 ttatcgtgat ttccgtgctg acgggcctct acacgatcat tggcggcctg ctggccgtcg
     8701 tcctcaccga atcgatccag accgtgattc tgctggtcgg cgccgtctgt gtcaccgccg
     8761 cgggactgtg gcgcgttggg ggctgggagg gcctgacatc ccatgtgacg gcggtcgaca
     8821 tgacggtgat tcgcggggga gacgatccct cggggatggc ctggtgggga gtttttctgg
     8881 gatatcccgt gatcggcatc tggtattggt gcgcggacca gacgatcgtg cagcgagtgc
     8941 tgggcgcgcg cgacgagaat cacgcgcgaa gcggcgccct cttcgccgga ttcattaaaa
     9001 tattgcccgt gttcctgttt gtctttccgg ggctcatctg caaggcgctc gttagccagg
     9061 gcgagtttgg ccccggtccc gaggatccgg cggaaacctt cccctacctc gtcaatcacc
     9121 tgctgccggt ggggctgacg ggcattgtcg cggccgggct gctggcggcg ctgatgagca
     9181 ccgtgtcggg agcgctcaac tcgatcgcgc tgctggtcag ttacgatatc gtgcggcgct
     9241 ggcgcccgca gacctcggaa ccggcgctcg tgacgatcgg gcgagtcgtt accttcgtgg
     9301 cgatggtgtg cgccatcctc tggtcgccgc tgctcggcac gaagttcaat acgctggcgc
     9361 aggcggccgc gacgatgatc tgctgcattg cgcccccgat caccaccatc tttctgtggg
     9421 gtgttttctg gcgcaaagct tcggcaatcg ccgcgggctg cacgatgtgg atcggcggcc
     9481 tgcttggcct gatcgtcttt ttgatgctgt tttttgaggt cgaatggtgg ccgatcggct
     9541 tcatgatggg cgccttctat ctgttcgtgg tgtgctcgat tctgctcgtc gtgctttccc
     9601 tcatttttcc tcaggcgcat accaccgaaa gcaaaagtct ggtgtggtcc agtttctggg
     9661 aaccgctgcg atcgccgggc tggccgggcc tggcgaacta ccgggtcgtg gcgggaatcc
     9721 tgtgtgtcgt gatggtgggc ctctacatct acttttcatg agggagggaa gaatcgaatg
     9781 ggtcttaaat cgggagtggg attggcgtgg atgatgttga ccgttctgct tggcggcaca
     9841 ggatgtcagc gcgcgcaggt catgaaaggg gaaatgatgc ctcaacgcta ctgtatgatt
     9901 ctcggactcc aggaaggagc attggatgaa tatgcggaat atcaccggga tgtctggccg
     9961 gacgtgctgg acgcacttcg aagcgcaaac ctgacgaatt acagcatcta cgtgttccat
    10021 gccgacgacg gtctgcccta tctggtcggc tatttcgaat atgtcggaag cgattttgag
    10081 agcgacatgg cacgcatcgg ggaattgccc gtgatcaagc gatggaacga cacgatgtcc
    10141 aaactgcaaa gaccgattga taaccggaag gacggcgaat ggtgggccgc cgccgaggag
    10201 ttgttccaca tggagtgacg gcgacaccgt gcgggacaag taggaaagtc ggaaaaagga
    10261 cagatgaaat gaagcattcc gaaacaacgc cttccctgga aacgacggtt caccccggtg
    10321 ccgatgggcc gccattcagc cacgccacga gcctgcggca gcttggcatg atcgtttcgc
    10381 acacgcactg ggacagggaa tggcgttacc cgatctggga gacgcggttt catcttcaac
    10441 agatgatgga tgaactgatc gctctgctgg aaaagggagt ctattcgaat tttctgctgg
    10501 atggacagac agtcatcgtc gacgactacc tgatgtttcg gcccgacatg gcgcgccgca
    10561 tcgaggcact ggttcgcgac ggcaagctga gcatcgggcc gtggtacacc cttccggacc
    10621 tgagcgtgat cgatgcggaa tgcgtcgttc gcaacctgct ctgggggcgc cgccgcgccg
    10681 atgcgctggg cggctgcatg aagatcggct acactacgtt cggctggggt cttccggcgc
    10741 agttcccaca gattcttgct ggcttcgaca tccgctttat cacaatcagc aagtatctgg
    10801 atccccggcg gctggagcac aatgaatttg tgtggcgggc gcccgacggc acggaggcgt
    10861 tggcgaccat catcggcgag catgcccgcg ccgggttttt cttcagttgc tatcaggaca
    10921 tcctcgtggg gcgccgctat ctcggcgaag actggaaata cgacttctcc gccggtcgcc
    10981 tgccgtttca ccgtgccgat ccaccggccg cgcaccgtga ctattgcctg ctggagccgc
    11041 cgagggggct tcatcccgaa ctggcgaacc gttcaacggt tgaccagtgc atgcgcaaca
    11101 ccgacggctc gctgatgcgc cacacgcggt tgatgatgaa cggcaccgac agtatcttcc
    11161 cttcctcgat ttttcccaaa ctcgttgaga cggtcaatca ggtggacccc gacaggcagt
    11221 tcgtccaggc atcgctcgcc gagtatgtgg acgtgctgca ggcggaactg caccaggaac
    11281 acctgcaggt tgtgcagggc gaactgcgcg acggtcccgc ggcgaaggtg agcggcaacg
    11341 cgctatccac gcgattgtac atcaagcaga tgaatcggcg ggcggagaac gcgttgctgc
    11401 gcaacgcgga acccttggct gtcgcatcct ggttgagcgg gcaggaatac cccgaggagt
    11461 tcctgaacaa ggcctggaac ttcatgctga aggctcactg ccacgactcg atcaatgggg
    11521 tcacgctgga taagacggcg cgtgatacgg tcggccggct tgatcaggtg ctggatctcg
    11581 ccaacgggac gatcaattcg gcgttacgct acatcgtgcg ccagatgaat ctggtgatcc
    11641 aggatgagag ggagacgctg ctggtcattt tcaactgcct gccgcgccag cgacgcgagg
    11701 tcgcggtcgc gtacgtggat ttcccggtgg attggaatgt cgatttgttc gaatttgtca
    11761 atcccgaggg ggagcgcctg gccgttcaga cgttgaacaa atcgattatt aacgtctcgg
    11821 cgtggtccga tctgcaccgg gcgttcccat ttcatgtcga gcgccacgaa gttgcctttg
    11881 ataccgggcc gctcgccgcc tgcggatata ccgtgctgcg cgccgtgccg acgcgcttca
    11941 aagaccgaac gaaggtgatc tggcatgatc cggttcccgt gaggaatcaa atcgcgccta
    12001 actcgagaac gctggaaaac gaatgcgttc gcattcagat caatgagaat gggacctttg
    12061 atctgcacga caaagttaac cagacggtct accgcgggct gaactatttc gaggacatcg
    12121 gggacgcggg cgactactgg gtcagaacgg aaccctgccg caatcggacg gtcaacagca
    12181 ttgggtcgcg cgcccggatt gaactgattg aaaacggcga actgcgggca tcgatcgcgg
    12241 tgaccgtgtc gctgcacgtt ccggatcggg acgcccagga tgatttatcg cgggaacttg
    12301 acctccacta tcgcgtaacc cttcaagccg gcaggccgga ggttgaggtg gaagtgtggt
    12361 tcgagaacag gattcccaac caccggctgc aggtgatgtt tccgacgggc ctgagcgacg
    12421 ccgtgcggat tgtcgccgag acgcactttg cgactaatcg caagccgttc ctgatcaagc
    12481 ggctcgagga tgacgccttt tacctcgaca tgcaatcgca gccgatgggc cggtttctgg
    12541 acattgcgga tcgcgagcga gggctcgcga tcatctcgaa ttcgctgacg gagtatcaag
    12601 gctgtaatga ccaggaaaag acggtcggac tgacgctgct gagatccgtc aagaacgaaa
    12661 tgtgcgccga gtttcgtacc gtggcggaaa acgcggatga gaacggcggg cagtgcttcg
    12721 gccgtcatcg ggcaagctac acgctgctgg cgcacggcgc ggacgcgacg caggccctca
    12781 tcgcgcaatt ggccgaccga cacgggacgc cgctggttat ggtgcaaacc atggcaaaca
    12841 cggggccgtt gcccgggacc cgattcagct ggttcgaatt gatcggcgag cgcgttcgtc
    12901 tatcgacgat caagcgcgcc catgatcgac cgagtgtaat cgtcaggctg tataatccga
    12961 cggacgccga agcgtgtgcg gaactgcgct cggccatgcg gctgacggcc tcctggatca
    13021 cggacctgaa tgaagagcgg aagcgcgaac ttgaaattct gaacgaccgg caggttcgac
    13081 tgacgctgga gcaccacaag attgcgaccg tggaactggt tgtcgaccgc gactgatgca
    13141 gacacagcac gcgcattcga agtaaggagg aaccggacga tgaaacggtc aaccatcaac
    13201 cgctacatcc gggcgaccga ggcctttttt gcggagcggc aattccacct tcccgatttt
    13261 ggccactgga ctcccgaaga ctggcgtcaa aaagggcccg agacaaggga aattgccgaa
    13321 tgccaattgg gatgggacgt caccgacttt gggctgggag atttcgagaa aacgggtctg
    13381 gtggccttta cggtccgtaa tgggctcaac caacaacttt cggcgggagg gaaaccctac
    13441 tgtgagaaga tcatgcgggt gggtgaggct caggtgacgc catggcattt tcattggaat
    13501 aagatggaag acattatcaa tcgcggggga ggaaacctga ttatcgctgt ggcgaatgtc
    13561 agcgaggacg aggggcttgc cggcacgccc gtggaattgt cgatcgacgg cgtcagacat
    13621 acggtcccgg ccaggacgga agtgtcattt gcgcccggcc agagtctcac gattcccccc
    13681 tatttgtatc atgcgttctg ggcggaaccg ggctcgggac ccgtgctgtg cggtgaggtg
    13741 tcaaaagtca atgatgatca tcatgacaat cgatttttaa aggcgatccg gttttccgag
    13801 attgaggaag atgagtcgcc ttaccgactg attgtgggcg actatgagcg ctactattcc
    13861 gcattgtcgt cataatgcct gacgggatcc cagcgcgcaa cacgtggccg acgcgtggca
    13921 cgagacggtc gagcgcacgt ccgcgatgat tcacgcatga gcgccggcgc caggcgaggg
    13981 ggcggcgctc gcgcgcggcg gagcatgagc aagcccgatg acgcttcaaa acggccgacg
    14041 gatttttgcc agctgaaagt taggaggtga tgcatgaatc cgccatttga catgctacga
    14101 tgtgttattc tgttcatggt tctgacaacg tgtggatggg cgcaagccca gcatgaattt
    14161 catgtggctc cggacgggga cgacgacagc gcgggatcgg tcgacgcgcc gttccggacg
    14221 ctggaacggg cgcaacgggc ggtccgagaa gtcaaccaac gcatgacgca ggatattcat
    14281 gtgtatctgc atgaggggac ataccgtctg gacgaaactc tgacgttcaa gcccggggat
    14341 tcggggttca atggtttcgc ggtggtgtat cggaacgctc cgggtgaacg cgccgttgtc
    14401 agcggcggat ccgttgtcag caactggcgg ctgcatgacg cggagaaagg gatctgggtt
    14461 gcacccgcgc cgcccggcgt cggctttcgc cagttgtatg tcgatggaaa gcgggcaatc
    14521 agggccaggt catctcaggg attgcccgtg ggaaccgaga aacgagcgtc tggttattca
    14581 cttccgaacg acagcatgtc gacctggaga aatgttgatc agctcgaatt cgtcttccgt
    14641 gagcaatggc gtgagccgcg gtgcctggtt gcgtcgatcg acgggacatc aatcaccatg
    14701 caacagcccg gatgggcgat gtgcaacagc tgggggatct acacctcggt cttcaatgtc
    14761 gattatccga tctacatcga aaacgcctat gagttgctgg accagcccgc ggaatggtat
    14821 ctcgaccgcg cggaagacaa ggtctacctg ataccgttcg ggtccgctcg accgggggag
    14881 attgaggtcg tcgcgcccat tctggagcgg ctgatccgat ttgacggtac ggccgtggat
    14941 cccgtgcacg acatccgtgt tgagggcctc gtcttatgcg atgccacgtg ggacatgggc
    15001 gccccggcat acggataccc ctcgatccag gcgaccgtga tgggcgcggc gggccaggag
    15061 aacgtccatg gaactttgaa tgagaagcat caggtgcccg gcaacatcac gctcgcgggg
    15121 gcgcaaaagg tgaagtttat tggcaatacg ttcacccggc tggcggccgt cgctgtggag
    15181 ctggtgtcgg cgtgttccga ttgccagttc attggcaatc tgttcacgga aacatcgggt
    15241 ggcgcgatgc aactggggcg ctacaatgag ccgtacctgc cgagcgagga aacgatcaga
    15301 aacacgctgt ttgagaacaa ttacattcac gatgtcgccc tggactaccg cgatcatccc
    15361 gcgatcacgt gcttcttcac ggactcaacg gtgatccagc acaacgaaat tgagcgcgtg
    15421 ccctacagcg caatctccat gggttggggt tggggccggc tggaggagaa gcacggccag
    15481 acgatcgccc ggaacaacca gattctgaat aatcatatca gcaatcacat gctcgtcatg
    15541 gaggacggca gcgcgatcta ctgcctgggc tggcagccgg agttgctgat cgaggggaat
    15601 cacatccatg acacgggggc ggtcatctcg ccggggatct atctggatcg cggttgccgc
    15661 tacatgacgg tgcggaacaa cgtgatctac aactgtgagt cgaacatgat ggtcaagggg
    15721 acggatcacc tgatcgaata caatttctgg caggatgagt tcagcacgcg gcgtccgggg
    15781 gcgccgtcgc actgggtcta ctggggcgtc gccacgctgt attggaagat cgcccataac
    15841 gttgtcgtca tggacgaggc gtccatgccc gcgtcgatca ttcagcaagc gggaatccgg
    15901 ccgcaagatg ctcacttgct cccggtccgg ggcgggctgg acagccaacc gcccaccgtg
//
LOCUS       DAOPCO010000062        15933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3741_length_15933_cov_6.207835, whole genome shotgun sequence.
ACCESSION   DAOPCO010000062 DAOPCO010000000
VERSION     DAOPCO010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3741_length_15933_cov_6.207835"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLS90_06475"
     CDS             complement(<1..1283)
                     /locus_tag="PLS90_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK02085.1"
                     /translation="MARLNSRTDPVAHGLALLLLILLLGLLIWPIVSVVRAGFVDREG
                     PTLFYFASIFDFSRGIAGNYYLEGLWNALRLATVTTLLCLAVALPLAWLMANHVFAGQ
                     KMLATLLLVPLILPPFVGALGLRRLLAPTNGPLVLLLRRFGLLGDTETLDLLAASPFL
                     ATAILMTLHLFPIVFLNLQAALANIDPALEEAAQNLGARPLHVFRRVTLPLIMPGLFA
                     GATICFIWSFTELGTPLMVGFRDVAAVQVFDGLTQIEQNPLPYALVTVLLLASVAAYA
                     AGRGVMGRAAFATAGKATVSRQARRLGPLATLAALAAVGLLICLAALPHLGVLSIAFA
                     ERWPATILPAEWTFEFMGQVLRHPLTGPSIANSLKYSALATLLNLTLGVAIGWIVVRT
                     TVRWRGALDGLAMLPLAVPGLVMAFGYLSITRRGMP"
     gene            complement(1452..3281)
                     /locus_tag="PLS90_06480"
     CDS             complement(1452..3281)
                     /locus_tag="PLS90_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02086.1"
                     /translation="MICPYCQTENRDDQETCYYCHKDISMLRLIVNKAKHHYNLALEH
                     AERDRVDEAITELKNALDLDASLVNARVVLGTLYARKEQFEDARQAWEQALDSDYRFR
                     KAHDYLHKAGQAEYVFPGMKRLQKVNLWLLATSAALALVLVILIIILALPDRERPQLV
                     RALGIARNDPLVSAATVEELAALSADEDTPPAIVDYARNMRVHIERAWEEDLAIARSA
                     LDHGAPLVTLEALNNLLSKDPNQTARTAAEELYAEAGARVADELRRASEAFRTNQLPY
                     DTFLGRAERVLELLPAGAAADQITSLTLEIKQTHLERMLEQARAAVADGTLNEAIALV
                     QEWRRNYPDHVAPLEEVLDQRMVTRTAMVEVQIEGLIESGQFDEASRVLADLGSLYKH
                     AGRPAPEEVLAGLRDAIGQARMAAARSAAEKAFAEQRWEDFLELTRRPTDLAADEAGL
                     AALGEQRARALQEVARGRWRWFDERKLAFQSLSITTEEAAQAIQWYGPVMEALPPDLT
                     WARGPLTFYTAAARLKLGDPQEARKLLARVHSDFDDSYVVDLVNQFQETFARELGLAE
                     EQPAPAASDEPTTGAATPPPAAEATPAADEEAPAPAEKASPAS"
     gene            complement(3459..5072)
                     /locus_tag="PLS90_06485"
     CDS             complement(3459..5072)
                     /locus_tag="PLS90_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02087.1"
                     /translation="MSKWSALPCLSLLSVAILAPPPAAAAPRPLDRFFGDRNNLLTER
                     VEIEGIVLPLREQPPQPVEVAQAAGLLPPAAIGQTPIVCLLDFQSETLSSDEQEALSQ
                     AVWARLRQLAGLRLLPREDTHRWLISHDLHPLMPYGDRVPLNEVAAALRADYLVTGHV
                     DQVQGIYTIDYSVFSARTGAPLVQDARHQRATIDEMVSYFGGLANEMRQVIVKDATGV
                     EVASVTAFHAPPPEEVAAGPAQAVPVDHKQAPARSGKTRLRGGEVVLEAPEHPAPAPA
                     RRAESPAPADPRPMEPLTTEIVEPATEPAPAPAERSEPPAPADEAPQLNEEIPPAPET
                     AATPAVESTPEAAAPEPEPEPEPEVEAEPAAPARSAAEKDAGADPARAEQLYEEALAL
                     SGDDQAEQRLEKIRQAAELDPTNPTYHQILANEYHRAGQFQKVIEHCDKALALAPNDS
                     MVLTIKASALFSLGRFQEAAAINKAAIEAKPDNLYARYNYALTLDELDSTDELAAWRE
                     YLQHAADDPTHNRLKLVDHARERVRQLEP"
     gene            complement(5307..6647)
                     /locus_tag="PLS90_06490"
     CDS             complement(5307..6647)
                     /locus_tag="PLS90_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02088.1"
                     /translation="MPRKIARRLCLLLLGVVPMISPAADPFYESSGGETPPPPASELR
                     WWKGNLHTHTLWSDGEEYPEMIVDWYKRNGYQFLALSDHNVFAESENFWIELARRPAA
                     AHHFPGYLARFGKDWVETDAADDASTFSRVRLKPLHEFRALFEEPGRFLLINSEEITD
                     RSGTGKSVHMNAHNLNEVIPPQGGATVRETIQNNLNAVKSQSERTGRDILPHLNHPNW
                     EWGVTAEDLAYVTDERFFEVFNGVLGVHNEGDELHADTDRIWDIINTIRIAELNAPPI
                     FGLAVDDAHHYHDTAFHNHLPGRGWIFVRSRYLTPEHIIRALRAGDFYASSGVTLRDF
                     QFDGETYQLEIEPEEGVTYVTEFFGTRQGFDPASEPVFDAAGVELTHRYSPDVGEVLQ
                     RSEGTRASYRLTGDELYVRARVTASVPIKYPYSDPPAPRRAWTQPVQPPARPAN"
     gene            6964..7827
                     /locus_tag="PLS90_06495"
     CDS             6964..7827
                     /locus_tag="PLS90_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02089.1"
                     /translation="MDAPPPLSVLVPCHNEEDSIAPTVDQLRQTLAETGAEFEIIVID
                     DGSTDRSAERVDPAKCRLIRLEHNRGYGAALKTGARAARHPWLAIIDADGTYQVGDLP
                     RLMRERGDCAMVVGARRGRNARIPAARRPAKWILNQLANYLTGARIPDLNSGLRVIRR
                     DLWERFEPLYPDGFSLTTTITLAALTNGYAVRHVPIEYRRRIGRSKIRPVRDTLSFVQ
                     LILRTVLYFDPLKVFVPLSLLLLLASIIVGLASKLSGEFMDVTTTALFVTGLQMLAIG
                     ALADLVTKRLP"
     gene            7839..9554
                     /locus_tag="PLS90_06500"
     CDS             7839..9554
                     /locus_tag="PLS90_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02090.1"
                     /translation="MPPREIYAAKALAQVPRLLSLEDRNPFSPTYGCCDREFWLCRST
                     DFPNAILQFGLHALALAWRHPMPGNIYYQNEKILRWTIAGMEYWMKIQKRDGSFDEFY
                     PNERGWAGPTGFLLYAMIDSYRLLGDAFPEKLKARFFEACEKAGRYLARWDEPGVLAN
                     HHAMAVLPVFEAARLLGTSELWDGYEAKLEEFYSYCDPEGWCLEYDGADPGYLSATVS
                     FLAKIWKHRGEAPRVARDERMINVLRRAVEFCAWFAYPNGHYAGTIGSRQTLHFYPHG
                     FELLAREIPLAGAVADHMLEGLRAGALVPPEIQGDRYYQYRVPEFLLSYVDCQERPYT
                     PADRPKLPWETVPREEYYPRGRFFMRRTERTYLVVNLAKGGVAKQFDVQRRKLIFNDC
                     GIVARLDDGRLVTSQWIDPAYQVKAERNRLEIVGISHRIPTKYFNPYTFILFRVFMLA
                     FGWNTRVAYQIKGLIRRLLMTRAQTMPLRFRRVIQISGDELELTDTVALENCRVRRLW
                     IGDEMPVRYVPQSRYFQPQELEINGWEAPPEVIARLNRRGGLTIRRTIRPVPEGPAAV
                     ELSLE"
     gene            9564..10610
                     /locus_tag="PLS90_06505"
     CDS             9564..10610
                     /locus_tag="PLS90_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPK02091.1"
                     /translation="MAGPVSRSLRWLRWIGPLLLILLILWAGPDRLWRAARAGNPWWL
                     LFGWVLNFPQLGLKALRWQRIVRWQGIPLPYRNAFLSYFGSLLVGFLTPGRLGEMAKA
                     FTLRHDYGVPLAAAFSSVILDRLFDLYLLMSLGALGIIRFGLVGSSLSWPIFIGLCAL
                     VFAPLLFLNEPVVRRLGGYLSGLGLLASRKALIQEKADQFATGLAVLGPARIAECVAL
                     TIAAYAVFFLQCYCCAWALGFYPGAVDMVLLMAATNFISFVPISISGLGTREACLIFF
                     LARLAEPQSPATAVAFGLMLFLVLFVGGGFIGFICWQWAPIGLRRSAEEVKKRSADKT
                     AEEYDRPGERRNSQ"
     gene            complement(10653..11012)
                     /gene="arsC"
                     /locus_tag="PLS90_06510"
     CDS             complement(10653..11012)
                     /gene="arsC"
                     /locus_tag="PLS90_06510"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638802.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HPK02092.1"
                     /translation="MGDKHAMLSIYHNPRCSKSRETLRLLRDEGHDPRVIEYLKEPPT
                     AAQLREIVRMLGGDAHRIVRSKEPAYAAAGLSRDSTDEEIIRAIVENPILLERPIVIN
                     RGKAALGRPPEQVLEIL"
     gene            complement(11133..15473)
                     /locus_tag="PLS90_06515"
     CDS             complement(11133..15473)
                     /locus_tag="PLS90_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK02093.1"
                     /translation="MLHPSHIAIHRPVTTLMVTLALVFLGFISYRELAVQRFPDLTLP
                     AIGYRVYLTEGTWSPEETNELITIPFEKIVAAMPGVREMRSTSMEGSMWGYARFAQGS
                     DMRFRLIDLQDRVNKWINEQEERRIEYQLDLWSTDQVAAELAQVIIEIPPGGEALTAL
                     AADRVARRLRSIDGVSRVEVAGNLLPNIALEAEQDPLRAAGFSVDQMLQAINARARGE
                     FWLGTLRIGSDRHEVKLYDPVRNLDDLLAIPLDRQGIHPVGSLTRLGRKVQEDETIYR
                     RNGRKAVEIVVNKERDRNTLRMSRIVRERLAEVQDELPPGFGIRIERDAAEDIEILFS
                     KLTRLAAAGALLSTLVLLLFVKSWRLALIVGLAIPSSIIMTFNLMYAAGLSLNILTLL
                     GLAAGVGMLVDNAIVVVENVFRHSQRTLDPRQAAWEGSREVTYALVISTATTLVVFVP
                     IFYIDDELVIFMKEPALSLVFPIAISLLVAMTLVPMLTSRVIGRRRSPRRSNGRLAWL
                     ARLDPWQRPGRPRRRLARELVLFLTKGCLRHPVRLIFTVLLIIFATFIASGLKVAVQS
                     QAGQDETRTIRLYGKLPLNLTLEEADAIFAAKEEQVRSRLAEAPAGEPVIASFASRFD
                     KRGGEIRLNIAKPYRRLPEAEFHAWLNDLVEGDETHGFRFRPFAAATLLQSGGSDLAR
                     SFFGGERLLVVGENIHAMIRTAEELKAYLEEQPEIQEAFTQIPTGEAEVVFDPDLDLF
                     RQLQVDVGSLQTLLRARDDRGVATSLVLRDEQQTERRVTVKVIPPESEQRPARARQTL
                     AELRKSRVAMQGGGSIPLENLGVFSVRHTAPAIVKENRQRNISVGFSFTPTLAHPQME
                     KERQNVLRRIQADLQARRFPPGVSATLAGTLETVGKQQVTFRKLVWLAIAMVYLVMAF
                     FFESLLSPLVILLTLPLAWIGGIWGIILFNTTLDEVAMLGVILLVGLVVNNGILLIDY
                     TRQRRRQGRCRRQRALLEAVSYRLRPIAMTSLTTILGLLPILFARDADREIRSLVSVV
                     IGGMISSALLTLVFIPVMYNVLSVLAERAAGVRARAGERARAGWGAIRNAGGRRRIRS
                     ALARVRPPAGAPTPLEIRIETISKIYPVFNRRQLLHLIPSREYAWGSRPPMGNRALDS
                     VSLTIRGGMFGLLGPNGAGKSTLMQILAGLVEPTYGNARVGGLDLRRDRDALRGLISY
                     LPQNFGVYPRLTLNQYLNFFAALHGLRDDARRRRRIDEVAEMVGLLAVRDKPLSQLSG
                     GMRQRAGIAQLLLDPRPIMIVDEPTAGLDPVERVRFRLLLSELARDRIVILSTHIVDD
                     ITSSCREIAILNFGQVVYQGDLDGVLTRAAGRVWDLHLPHHAPQPMPNRHVLYKKHVG
                     DQILYHYFAIEPIPGSAAAPPNFEDAYVALLVEHELSLKDRKMSERDGAAGGDRASRP
                     AIRSLIQRPT"
     gene            complement(15587..>15933)
                     /locus_tag="PLS90_06520"
     CDS             complement(15587..>15933)
                     /locus_tag="PLS90_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02094.1"
                     /translation="GEARGFPIYRIQAAEADQTTTGPEEITLYRVRARIRNDGGGRMP
                     VPLQLVTADGIINDRIWIGPDETVTWRFDCTSLPREVKVDPDGWIQRAPYFDGGWVTR
                     PVAPVRLELDDG"
BASE COUNT         2578 a         5212 c         5391 g         2752 t
ORIGIN      
        1 ggcatgccgc ggcgtgtgat ggagaggtaa ccgaacgcca tcacgaggcc ggggacggcc
       61 agcggcagca tcgccaggcc gtcgagcgcg ccgcgccagc gcaccgtcgt gcgcacgacg
      121 atccagccga tcgccacgcc cagcgtcagg ttgagcagcg tcgccagcgc cgaatacttc
      181 aggctgttgg cgatgctcgg cccggtgagc ggatggcgca gcacctggcc catgaactca
      241 aacgtccact cggcggggag gatcgtcgcc ggccaccgct cggcgaacgc gatggagagc
      301 acgccgaggt gcggcagcgc ggccaggcag atcagcagcc ccacggctgc cagcgccgcc
      361 agcgtggcca gcggcccgag ccgccgcgcc tggcgtgaga cggtggcctt gccggcggtg
      421 gcgaacgccg cgcgtcccat cacgccccgc cccgcggcgt aggcagcgac gctggccagc
      481 agcagcaccg tcacgagcgc atagggcagc gggttctgtt cgatctgcgt caggccgtcg
      541 aagacctgca ccgccgccac gtcgcggaag ccgaccatca gcggcgtgcc cagttcggtg
      601 aagctccaga tgaagcaaat cgtcgcgccc gcgaacagcc ccggcatgat cagcgggagc
      661 gtgacgcggc ggaagacgtg caacgggcgc gcgccgaggt tctgggccgc ctcctccaac
      721 gccgggtcga tgttggccag cgcggcctgc aggttcagaa acacgatcgg gaacaggtgc
      781 agcgtcatca ggatcgcggt ggcgaggaag ggcgacgcgg cgagcaggtc gagcgtctcg
      841 gtatcgccga gcaggccgaa ccggcgcagc agcagcacca gcggcccgtt ggtgggcgcc
      901 agcagccggc gcaacccgag cgctccgacg aatggaggca ggatcagcgg gacgagcagc
      961 agcgtcgcca gcatcttctg accggcgaag acgtggttgg ccatcagcca ggccagcggg
     1021 agggcgacgg ccaggcagag cagcgtcgtc accgtcgcca gccgcagcgc gttccacagc
     1081 ccctccaggt agtagttgcc ggcgatgccg cgcgagaaat cgaagatgct cgcgaaatag
     1141 aagagcgtcg gcccctcccg atcgacgaac cccgcgcgca ccacgctgac gatcggccag
     1201 atcagcagac ccagcagcag gatcagcaac agcagggcga gcccatgggc gaccgggtcg
     1261 gtgcgggagt tcaggcgggc catggttcgt tgtcgctcgg tgttcaggaa agagacatga
     1321 ggggcttaag gaagatccaa cgatgcgatg cccatgccgc ggtcctaaag gcattcatcg
     1381 acgtatttcg agagccgagg acgagggacg agagccgaat cttcaaagga cgaaaccaaa
     1441 ccgccgaagc ttcagcttgc gggcgaagcc ttctcggcgg gggcgggggc ttcctcgtcg
     1501 gcggccggcg tggcctcggc ggcgggcggc ggcgtcgcag cgccggtggt cggctcgtcc
     1561 gacgcggccg gcgcgggctg ctcctcggcc agccccagtt cccgggcgaa cgtttcctgg
     1621 aactgattga cgagatcgac cacatacgaa tcgtcgaagt cgctgtggac ccgcgcgagg
     1681 agcttgcgcg cctcctgcgg atcgcccagt ttgagccgcg ccgcggcggt atagaacgtc
     1741 agcggcccgc gcgcccaggt caggtcgggc ggcagcgcct ccatcaccgg gccgtaccac
     1801 tggatcgcct gggcggcctc ctcggtggta atcgagaggc tctggaaggc cagtttgcgc
     1861 tcgtcgaacc agcgccagcg tccgcgggcg acctcctgca acgcgcgtgc gcgctgctcg
     1921 ccgagggcgg ccaggccggc ctcatccgcg gccaggtcgg tcggccggcg cgtcaattcg
     1981 aggaaatcct cccagcgctg ctcggcgaag gccttctcgg cggcggaccg ggcggccgcc
     2041 atgcgcgcct ggccgatggc gtcgcgcaga ccggccagca cttcctccgg cgcggggcgc
     2101 ccggcgtgtt tgtacagcga tcccaggtcc gcgagcaccc tcgaagcctc atcaaactgt
     2161 cccgactcga tcaacccctc gatctgcact tcgaccatcg cggtgcgggt caccatccgc
     2221 tgatcgagga cttcctccag cggcgcgacg tgatccggat aattgcggcg ccattcctgg
     2281 accagcgcga tcgcctcgtt gagcgtccca tcggcgacgg ccgcgcgcgc ctgctccagc
     2341 atgcgctcca ggtgggtctg cttgatctcc agcgtcagcg aggtgatctg atccgcggcg
     2401 gcgccggcgg gcagcagttc caacacgcgt tcggcccggc ccaggaaggt gtcgtagggc
     2461 aactggttgg tgcggaacgc ctcgctggcg cggcgcagtt catcggccac gcgggcgccc
     2521 gcctcggcgt agagctcctc ggcggcggtc cgcgccgtct ggttggggtc tttcgacagc
     2581 agattgttga gcgcctcgag cgtcacgagc ggggcgccgt ggtcgagcgc cgaccgggcg
     2641 atcgccagat cctcctccca tgcccgctcg atgtggaccc gcatgttgcg cgcgtaatcg
     2701 acgatcgccg ggggcgtatc ctcgtcggcc gagagcgcgg cgagttcctc gaccgtcgcg
     2761 gccgacacca gcggatcgtt gcgcgcgatg cccaaggcgc gcaccagttg cgggcgttcg
     2821 cggtcgggga gggcaaggat gatgatcagg atgacgagga ccagggccag cgccgccgac
     2881 gtcgccagca gccacagatt gaccttctgc agccgcttca tccccgggaa aacgtattcc
     2941 gcctggccgg ccttgtgcag gtaatcgtgc gccttgcgga agcggtagtc gctgtcgagg
     3001 gcctgctccc aggcctggcg cgcgtcctcg aactgctcct tgcgggcgta gagcgttccc
     3061 agcacgacgc gcgcgttgac cagcgaggcg tccaggtcca gggcgttctt cagctcggtg
     3121 atcgcctcgt cgacccggtc gcgttcggcg tgttcgaggg ccagattgta atgatgcttg
     3181 gccttgttga cgatcagccg cagcatgctg atgtctttgt ggcaatagta gcaggtttcc
     3241 tggtcgtcgc ggttttcggt ctggcaatag ggacagatca tgaccggcgg ggctcgtgga
     3301 gggtcgccgc tccggccgga tcgatgcggc cggaggacgg cagaggaaga caccatcttc
     3361 taacggacgc cgccgcggtt tgcaatcttt cagttggccg gttcaggcgg tgcgggggct
     3421 gggacagaca caagcggaca tgaagggccg aggaggcatc acggttcaag ctggcggacg
     3481 cgctcgcggg cgtggtcgac cagcttgagc cgattgtgcg tcgggtcgtc ggcggcgtgc
     3541 tgcaggtatt cgcgccaggc ggccagctca tcggtggaat ccagctcgtc gagcgtcagc
     3601 gcatagttgt agcgggcata gaggttgtcc ggcttggctt cgatcgctgc cttgttgatc
     3661 gcggccgcct cctggaaccg tcccagcgag aagagcgccg aggccttgat ggtcagcacc
     3721 atcgaatcat tgggcgcgag cgcgagcgcc ttgtcgcagt gctcgatcac cttctggaac
     3781 tgtccggcgc ggtggtattc attggccaga atctgatgat aggtcggatt ggtgggatcg
     3841 agttcggcgg cctggcggat cttttcaagg cgctgctcgg cctggtcgtc gccgctcagg
     3901 gcgagcgctt cctcgtagag ttgttccgcg cgcgccgggt cggcgccggc atccttctcg
     3961 gcggcggacc gggccggggc cgccggctcg gcttcgacct cgggctccgg ctccggctcc
     4021 ggctcgggtg cagccgcctc aggcgtggat tcgaccgcgg gggtggcggc cgtttcgggc
     4081 gccggcggga tctcctcgtt cagttgaggc gcttcgtcgg ccggcgccgg gggttcggac
     4141 cgctcggcgg gggccggcgc cggttccgtt gccggctcga cgatttcggt cgtcagcggt
     4201 tccatcgggc gcgggtcggc cggcgcgggc gattcggcgc gccgcgcggg ggccggcgcc
     4261 gggtgctccg gggcctccag cacgacctcg ccgccgcgca gccgggtctt gccgctgcgg
     4321 gcgggcgcct gcttgtgatc gaccgggacc gcctgggccg gccccgcggc gacttcctcg
     4381 gggggcggag cgtgaaacgc cgtcaccgac gccacctcga cgccggtggc gtccttcacg
     4441 atcacctggc gcatctcgtt ggccaggccg ccgaagtagg acaccatctc gtcgatcgtg
     4501 gcccgctgat gccgcgcatc ctgcacgagc ggagcccccg tgcgcgcgga gaagaccgaa
     4561 taatcgatcg tgtagatccc ctggacctgg tcgacgtgac ccgtcacgag gtaatcggcc
     4621 cgcaacgccg cggcgacctc gttcagcggg acccggtcgc catagggcat caacgggtgg
     4681 agatcatgac tgatgagcca gcgatgggtg tcctcgcgcg ggaggagccg cagccccgcc
     4741 aactggcgca ggcgcgccca gacggcttgc gacagggctt cctgctcgtc gctgctgagg
     4801 gtctcggact gaaaatcgag cagacagaca atcggcgtct ggccgatggc ggcggggggg
     4861 agcaggccgg cggcctgggc cacctccacc ggctggggcg gttgctcccg cagcggcagg
     4921 acgattccct cgatctcaac ccgctcggtg agcaaattgt tccggtcgcc gaagaatcgg
     4981 tccagcgggc gcggcgcggc cgcggccgga gggggagcga ggatggccac ggataacagc
     5041 gacaggcagg gcagggcgga ccacttcgac atgatcggca gtctccttcg gcaactggag
     5101 ggtggcccgg ggaactgggc cgcaacggga tcggggtcag caacggcacg ccgatgtgtc
     5161 gactctagtt ctaatgcagc gcggccgggt acacaaagga aaagtggata cagggccgcg
     5221 ggcaacccgg cggtcaggtg tgggaagaac catggacagc aggacgcagg aagagtccct
     5281 tgcgtcgcct gtgttctttg cgtcgtttaa ttcgcgggcc gggccggggg ctgcaccggc
     5341 tgcgtccatg cccggcgcgg ggcgggggga tcgctgtagg gatacttgat cggcaccgag
     5401 gcggtgacgc gcgcccggac gtagagctcg tcgcccgtca gccggtagct ggcacgggtc
     5461 ccctcggaac gctgcagcac ctcgccgaca tccgggctgt aacggtgcgt cagttcgacc
     5521 cccgcggcat cgaacaccgg ctcgctggcg gggtcgaagc cctggcgcgt cccgaagaac
     5581 tcggtcacgt aggtgacccc ctcctcgggc tcgatctcga gctggtaggt ttccccatca
     5641 aactggaaat cgcgcagcgt gacgccgctg gaggcataga aatcgcccgc gcgcagcgcc
     5701 cggatgatgt gctcgggcgt caggtagcgg ctgcgcacga agatccagcc ccggcccggc
     5761 aggtggttgt ggaacgcggt gtcgtggtag tggtgcgcat catccacggc caggccgaag
     5821 atcggcggcg cgttcagctc ggcgatgcga atcgtgttga taatgtccca gatccggtcg
     5881 gtgtcggcat gcagctcatc cccctcgttg tgcacgccca gcacgccgtt gaacacctcg
     5941 aagaagcgtt catcggtgac gtaggcgagg tcctcggccg tcacccccca ctcccagttg
     6001 gggtgattca ggtggggcag gatgtcgcgg ccggtccgtt cgctctgcga cttgaccgcg
     6061 ttcaggttgt tctggatcgt ttcccggacg gtcgccccgc cctgcggagg gatcacctcg
     6121 ttcagattgt gggcgttcat atggaccgat ttgccggtgc cggaacggtc ggtgatctcc
     6181 tcgctgttga tcagcaggaa ccggcccggc tcctcgaaga gcgcgcggaa ttcatgcagc
     6241 ggcttgaggc ggacgcgcga aaacgtgctg gcgtcgtcgg ccgcgtcggt ctcgacccag
     6301 tccttgccga agcgggcgag atagcccggg aaatggtggg cggcggccgg cctgcgcgcg
     6361 agctcgatcc agaagttctc cgattcggcg aaaacgttgt gatccgacag cgcgaggaac
     6421 tgatagccgt tgcgcttgta ccaatcgacg atcatttcgg ggtattcctc gccgtcgctc
     6481 cagagcgtat gggtatgcag gttccccttc caccagcgaa gctcgctcgc cgggggcggg
     6541 ggcgtctcgc cgccgctgct ctcgtaaaat ggatcggcgg cgggagagat catggggaca
     6601 acgccgagca gcagcaggca caggcggcgc gcaatcttcc gtggcatggc tcgaatcctc
     6661 gcgggtgggc tgaatctggg cgaggggaga cgaatcgctc cggcacccgc cattgactat
     6721 agagaagaac ggccggaaat tccagcggtg gccgccgcgt ttaggtcgcc tgggacgtcg
     6781 gccgtttcgc ggggaaggtc cgaaaaccag tccgtattca tccagcgcaa cacgcgcgcg
     6841 gcgaaatcag tcgcccgccc ggtttcttga cggcgcgggg ggcatcgact agactccgca
     6901 cgaagtcccg ccgggcgtca tcatccgtcc gcgtcacgat ccgtccggag ccaggtcccg
     6961 ccgatggatg cgccgccgcc gctcagcgtt ctcgttccct gtcataacga agaggactcg
     7021 attgcgccga cggtcgatca gcttcgccag acgctggccg agaccggcgc cgaattcgag
     7081 attatcgtca tcgacgacgg atcgaccgac cgctccgccg agcgcgttga ccccgcgaaa
     7141 tgccggctga tccggctgga acacaatcgg ggctacggcg ccgcgctcaa gaccggcgca
     7201 cgcgcggccc gccacccgtg gctggcaatc atcgacgccg acggcacgta tcaggtgggc
     7261 gacctgccgc ggctgatgcg cgagcgcggc gattgcgcga tggtcgtggg ggcgcgccgc
     7321 ggacgcaacg cgcggatccc cgcggcacgc cggcccgcca aatggatcct caatcaattg
     7381 gccaattacc tgaccggcgc gcgcattccc gatctgaact cgggtctgcg cgtcatccgc
     7441 cgcgacctgt gggagcggtt cgaaccgctt tatcccgacg gcttttcgct gacgacgacg
     7501 atcacgctgg ccgcgttgac caacgggtac gccgtccggc acgtgccgat tgaataccgc
     7561 cgccggatcg gccgctccaa gatccggccc gtgcgcgata ccctttcctt cgtgcagttg
     7621 atcctgcgca ccgtgctcta tttcgatccg ctcaaggtgt ttgttccgct gagcctgctg
     7681 ctgctgctgg ccagcatcat cgtcggcctg gccagcaagc tgtcgggcga attcatggac
     7741 gtgaccacga cggcgctgtt cgtcaccgga ttgcagatgc tggcgatcgg ggcgctggcc
     7801 gatctggtca cgaaacgact gccatgacgg aaaaccccat gcctccgcgc gaaatctatg
     7861 cggccaaggc gctggcgcag gtcccgcggc tgttgtcgct cgaggatcgc aacccctttt
     7921 cgcccaccta cggctgctgc gaccgggagt tctggctctg ccgttcgacc gatttcccca
     7981 atgcgattct gcaattcggc ctgcacgcgc tggcgctggc ctggcgccat ccgatgccgg
     8041 gcaatatcta ttatcaaaac gaaaagatcc tgcgctggac catcgccggg atggaatact
     8101 ggatgaaaat ccagaagcgc gacggctcgt tcgatgaatt ctatcccaat gaacggggct
     8161 gggcggggcc gacgggattc ctgctttatg cgatgatcga ttcatatcgg ctgctgggcg
     8221 atgcgtttcc ggaaaaatta aaggcgcgat ttttcgaggc ctgtgaaaag gccggccgct
     8281 acctggcgcg ctgggatgag ccgggcgtgc tggccaatca tcatgccatg gccgtgctgc
     8341 ccgtgttcga agccgcccgg ctgctcggaa cgagcgaatt atgggacggc tacgaggcca
     8401 aactcgagga attctattcc tattgcgacc cggaaggctg gtgcctggag tatgacggcg
     8461 ccgacccggg ttatctttcg gcgacggtca gctttctggc gaaaatctgg aaacaccggg
     8521 gcgaggcgcc gcgcgtcgcg cgggacgaac ggatgatcaa cgtcctgcgc cgcgcggtgg
     8581 aattctgcgc ctggttcgcc taccccaatg gccactatgc tggcacgatc ggcagccggc
     8641 agacgctgca cttctatccg cacgggttcg agttgctcgc gcgcgaaatc ccgctggcgg
     8701 gcgccgtggc cgatcacatg ctcgaggggc tgcgcgccgg cgcgctggtt cctcccgaga
     8761 ttcagggcga ccggtattac caatatcgcg ttcccgaatt tctgttaagt tatgtcgact
     8821 gtcaggagcg gccgtacacg cccgctgacc ggccgaagtt gccgtgggaa acagttcccc
     8881 gcgaggaata ttatccgcgc ggccggtttt tcatgcgccg gacggagcgc acctatctgg
     8941 tggtcaacct ggccaagggg ggcgtcgcga aacaattcga cgtccaacgc cggaaactta
     9001 tttttaatga ttgcggcatc gtggctcggc tcgacgacgg ccgcctcgtc acctcgcagt
     9061 ggatcgaccc ggcctatcag gtgaaagccg aacgcaaccg gttggagatc gtcggcatca
     9121 gccaccggat tccgaccaaa tattttaatc cgtatacttt tattctgttc cgggtcttta
     9181 tgctggcctt cggctggaat acgcgggtcg cctatcagat caaggggctg atccggcggc
     9241 tgctgatgac gcgggcgcag acgatgccgc tccgatttcg ccgggtcatc cagatcagcg
     9301 gcgacgaact ggaactgacc gacaccgtgg cgctggagaa ttgccgcgtg cggcggctct
     9361 ggatcggcga tgaaatgccg gtgcgctatg tcccgcagtc gcgctatttt cagccgcagg
     9421 aactagagat caacggctgg gaggcgccgc cggaggtcat tgcgcggctg aaccgcaggg
     9481 gaggcctgac gatccggcgc acaatccggc ccgttccgga ggggccggcc gccgtcgaat
     9541 tgtcattgga ataagaggcc gtcatggcgg ggccagtttc acgttccctg cgctggctcc
     9601 gctggatcgg gccgctgctg ctgatcctgc tcattctgtg ggccgggccc gatcggctct
     9661 ggcgcgccgc gcgcgcgggc aacccgtggt ggctgctctt cggctgggtg ctcaatttcc
     9721 cgcagctcgg actgaaggcg ctgcgctggc agcggatcgt ccgctggcag ggaatccccc
     9781 ttccctatcg gaatgccttc ctctcctatt tcggcagtct gctggtggga tttttgaccc
     9841 cggggcgttt gggcgaaatg gcgaaggcct tcaccttgcg ccacgactat ggcgtgcccc
     9901 tcgccgccgc gttttccagt gttattctcg accgcctgtt tgatttgtat ctgctgatga
     9961 gtctcggcgc gctcggcatt atccggttcg gcctggtcgg gtcgtcgctc tcgtggccga
    10021 ttttcattgg cctttgcgcg ctggttttcg cgccgctcct ctttctcaac gaaccggtgg
    10081 tgcggcgact cggcgggtat ctttcgggtt tgggcttgct ggcctcgcgc aaggcattga
    10141 ttcaggagaa agcggatcaa ttcgcgaccg ggctggccgt gctcggtccg gcgcggatcg
    10201 ccgaatgcgt cgcgctgacc atcgccgcct acgccgtctt ctttctgcaa tgctattgct
    10261 gcgcatgggc cctggggttt tatcccggcg ccgtggacat ggttctgctc atggcggcga
    10321 ccaatttcat cagctttgtg ccgatttcca tttcgggtct ggggacgcgc gaggcgtgcc
    10381 tcatattttt cctcgcccgt ctggccgaac cgcagtcgcc ggccaccgcc gtcgcgttcg
    10441 ggctgatgct gtttcttgtt ctgttcgtcg gcgggggttt tattgggttt atctgttggc
    10501 aatgggcgcc gatcggcctg cgccgttcag ccgaggaagt gaaaaaacgt tcggccgaca
    10561 agacagcgga agaatacgac cggccaggcg aacgacgaaa ttcacagtga acaacgtcga
    10621 atttctctgt ctcctggcgc ctcctcggtc cgctacagga tttccaatac ttgttccggc
    10681 ggtcggccca gcgccgcctt tccgcggttg atgacaatcg gtcgttcaag caggatcgga
    10741 ttttcaacga tcgcgcggat gatttcctcg tcggtgctgt cgcgcgaaag acccgccgcg
    10801 gcgtaggccg gttccttcga ccggacgatc cgatgggcat cgccgcccag catgcgcaca
    10861 atctcacgaa gctgcgccgc cgtcggcggc tccttgaggt attcgattac ccgcgggtca
    10921 tgaccctcat cgcgcagcag gcgcagcgtc tcgcggctct tggagcagcg cggattgtga
    10981 tagatcgaca gcatggcgtg tttatctccc atctctcgcg tgttttcggt gccctgggtc
    11041 ggaggtatcc gcatccaccg ccatcgaatt tacagcactc ggcggcgggt gggaagcggc
    11101 cgcccgcccg cacttgacgg gcgaaaatgc ccctacgtcg gtcgttgaat cagcgaacgg
    11161 atcgccggcc ggctggcccg gtcgccgccg gccgccccgt cccgctcgct cattttgcga
    11221 tccttcagac tcagttcatg ctccaccagc agcgcgacgt aggcgtcctc gaaattcggc
    11281 ggcgcggcgg cggagccggg aatcggctcg atcgcgaagt aatgatacag aatctggtcg
    11341 ccgacatgct tcttatagag cacatggcga ttgggcatcg gctgtggcgc gtgatggggc
    11401 aggtgcaggt cccagacgcg accggcggcg cgcgtcagca ccccatccag atctccctga
    11461 tacacgacct ggccgaaatt caggatggcg atctcgcgac aactgctggt gatgtcgtcg
    11521 acgatgtgtg tggagagaat gacgatccgg tcgcgggcga gctcgctcag cagcagccgg
    11581 aaccggacgc gttcgaccgg atcgagcccg gccgtcggtt catccacgat catgatcggg
    11641 cgcgggtcaa gcagcagctg ggcgatgccg gcgcgctggc gcatgccgcc cgacagctgg
    11701 ctcagcggct tgtcgcgcac cgcgaggagg cccaccatct ccgccacctc gtcgatccgc
    11761 cggcgccgcc gcgcgtcgtc gcgcaggcca tgcagcgcgg cgaagaagtt gaggtattgg
    11821 ttgagcgtca gccgcgggta gacgccgaaa ttctgcggca ggtagctgat caggccgcgc
    11881 agcgcgtcgc ggtcgcggcg cagatccagc ccgccgaccc gcgcgttgcc gtaggtcggc
    11941 tcaaccagcc cggccaggat ctgcatcagg gttgatttgc ccgcgccgtt ggggccgagc
    12001 aggccgaaca tgccgccgcg gatcgtcagc gacacgctgt ccagcgcgcg gttgcccatc
    12061 ggcggccggc tgccccaggc atattcgcgc gacgggatca ggtgcagaag ctgccgcctg
    12121 ttgaacacgg gatagatttt gctgatggtt tcgatccgga tttcgagcgg ggtcggggcg
    12181 ccggcgggcg gccggacccg tgccaacgcc gagcgaatcc gtcgccggcc gcccgcgttt
    12241 cggatcgccc cccagccggc ccgggcccgc tcgcccgccc gcgcccgcac ccccgccgcg
    12301 cgttcggcca gcacgcttag cacgttgtac atgacgggga tgaagacgag cgtcagcagc
    12361 gcggagctga tcatcccgcc gatgacgacc gagacgagcg agcggatttc gcgatcagca
    12421 tcgcgggcga acaggatcgg cagcagcccc aggatcgtcg tcagcgaggt catcgcgatc
    12481 ggccgcagcc ggtaactgac cgcctccagc aatgcgcgct ggcgccggca tctcccctgg
    12541 cgccgccgct gccgcgtgta gtcgatcagc aggatgccgt tgttgacgac gagcccgacg
    12601 agcaggatca cgccgagcat cgcgacttca tcgagcgtcg tgttgaacag gatgatcccc
    12661 cagatgccgc cgatccacgc cagcggcagc gtgagcagga tcaccagcgg gctgagcagc
    12721 gactcgaaaa agaacgccat caccaggtag accatcgcaa tggcgagcca gacgagcttg
    12781 cggaacgtta cctgctgctt gccgaccgtc tccagcgtcc ccgccagcgt cgcgctgacc
    12841 cccggcggaa aacgccgcgc ctgcaggtcg gcctggatcc ggcgcagcac gttctggcgt
    12901 tccttctcca tctggggatg ggccagcgtg ggggtgaagg aaaacccgac cgagatgttc
    12961 cgctggcggt tctccttgac gatggcgggg gcggtgtggc ggacggagaa gacgccgaga
    13021 ttctccagcg ggatcgagcc gcccccctgc atcgccaccc gcgacttgcg cagctccgcc
    13081 agcgtctgcc gcgcgcgggc cgggcgttgc tcgctttcgg gcggaatcac cttcaccgtc
    13141 acccgccgtt cggtttgctg ctcgtcgcgc aggacgagcg aggtcgccac gccgcggtcg
    13201 tcgcgcgcgc gcagcagcgt ctgcagcgag cccacatcca cctggagctg ccggaacagg
    13261 tcgaggtccg gatcgaagac gacttcggcc tcgcccgtcg ggatctgtgt aaacgcctcc
    13321 tggatctcgg gctgttcctc caggtaggcc ttcagttcct cggccgttcg gatcatcgca
    13381 tggatgttct cgccgacgac gaggaggcgt tcgccgccga agaacgagcg cgccaggtcg
    13441 ctcccgccgg attgcagcag cgtcgcggcg gcgaacggac gaaaacgaaa cccgtgcgtc
    13501 tcgtcgccct ccaccaggtc gttcagccag gcgtggaatt ccgcctcagg gagtcggcga
    13561 tacggtttgg cgatgttcag ccggatctcg ccgccgcgtt tgtcgaaccg gctggcgaac
    13621 gacgcgatga ccggctcgcc cgccggcgcc tcggccagcc ggctccgcac ctgctcctcc
    13681 ttcgcggcga aaatcgcgtc cgcctcttcc agcgtcaggt tgagcggcag cttgccgtac
    13741 agcctgatcg ttcgcgtctc gtcctgaccc gcctggctct gcacggccac cttcagcccc
    13801 gaggcgatga aggtggcgaa aatgatcagc agcacggtga aaatcagccg gacggggtgg
    13861 cgcaggcacc ccttcgtcag aaacaggacg agttcgcgcg ccagccggcg ccgcgggcgc
    13921 ccgggccgtt gccaggggtc gagccgcgcc agccaggcca gccggccgtt cgatcggcgc
    13981 gggctccgcc gccgcccgat cacccgcgag gtcagcatcg gcaccagcgt catcgccacc
    14041 agcaggctga tcgcgatcgg aaagaccagc gacagcgccg gctccttcat gaagatcacc
    14101 agctcgtcgt cgatgtagaa gatcggcacg aagaccacga gcgtcgtggc ggtcgagatc
    14161 accagggcgt acgtgacctc ccgcgagccc tcccaggcgg cctgtcgggg atcgagcgtc
    14221 cgctgcgaat ggcgaaacac gttctccacg accacgatcg cgttgtcgac gagcatgccg
    14281 acgccggcgg cgagccccag cagcgtcagg atgttgagcg acagcccggc ggcatacatc
    14341 aaattaaacg tcatgataat ggacgagggg atggccaggc cgacgatgag cgccagccgc
    14401 cagctcttga cgaacagcag cagaaccagc gtggagagca gcgccccggc ggccgccagc
    14461 cgcgtcagct tgctgaacag gatttcgata tcctcggccg cgtcgcgttc aatccggatc
    14521 ccgaatccgg ggggcagttc atcctgaacc tccgccagcc gctcgcggac gatccgcgac
    14581 atccggagcg tgttgcggtc gcgttccttg ttgacgacga tctcgaccgc cttgcggccg
    14641 ttgcgccggt aaatcgtctc gtcttcctgc accttgcgcc cgaggcgcgt cagcgaaccg
    14701 accggatgga tcccctggcg atcgagcggg atcgccagca gatcgtccag gttgcggacc
    14761 ggatcgtaga gcttgacctc gtggcggtcc gagccgatcc gcagcgtgcc gagccagaac
    14821 tcgccccgcg cgcgcgcatt gatcgcctgc aacatctggt cgacactgaa cccggcggcg
    14881 cgcaacgggt cctgttcggc ctcgagcgcg atgttgggca gcaggttgcc ggctacctcg
    14941 acgcgtgaaa cgccgtcgat gctgcgcagg cggcgcgcca cgcggtcggc cgccagcgcg
    15001 gtcagcgcct cgccgccggg cgggatctca ataatcacct gcgccagctc ggccgccacc
    15061 tggtcggtgg accacaggtc gagctgatat tcgatccggc gctcctcctg ttcgttgatc
    15121 cacttgttca cccggtcctg caggtcgatc aggcggaatc gcatgtcgct cccctgggca
    15181 aagcgggcgt agccccacat cgacccttcc atgctcgtcg agcgcatttc gcgcacgccg
    15241 ggcatcgccg ccacgatctt ctcaaacggg atcgtgatga gttcattcgt ctcctcgggc
    15301 gaccacgtcc cctcggtcag atagacccgg tagccgatgg cggggagcgt cagatcggga
    15361 aagcgctgca ccgccagttc gcggtaggag atgaagccga gaaagaccag cgccagcgtg
    15421 accatcagcg tggtcaccgg gcggtggatg gcgatatgag acgggtggag catgggctgg
    15481 cgcaagggtt gccgaatcaa ggacgccagg gacatgaggc aaggtcaacg accatcccgc
    15541 cgtccgacgc ctattgtgtc ctttcctgtc gtttcgtgaa ccatcgttac ccgtcgtcca
    15601 gttccagccg cacgggggcg accggacggg tcacccagcc gccgtcgaaa tacggcgcgc
    15661 gctggatcca gccgtccgga tccaccttca cctcgcgcgg cagactcgtg caatcgaacc
    15721 tccacgtcac cgtctcgtcc ggcccgatcc agatccgatc gttgatgatg ccgtcggccg
    15781 tgacgagttg cagcggcacg ggcatccgcc cgccgccgtc gttgcggatc cgcgcccgca
    15841 cgcggtagag cgtgatctcc tccggcccgg tcgtcgtctg gtcggcttcg gccgcctgaa
    15901 tgcgatagat gggaaatccg cgagcctcgc cgg
//
LOCUS       DAOPCO010000063        15908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3750_length_15908_cov_5.759982, whole genome shotgun sequence.
ACCESSION   DAOPCO010000063 DAOPCO010000000
VERSION     DAOPCO010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15908
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3750_length_15908_cov_5.759982"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PLS90_06525"
     CDS             complement(<1..1518)
                     /locus_tag="PLS90_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPK02095.1"
                     /translation="MSSLLLPIIPLKEIVVFPNSITPLFIVRHRSLQALEGALAQDKA
                     VFLVTQKNVEEEHPGLEDLYEVGCVAGIVQVLRLPDGSAKILVEGRYPARIVRATSEP
                     SYMTALVEEIHAVEERGKEIEALRRTVEKQFETYVQLSDKVPEDLAVSIRSLDDAVPL
                     ANAITNYCHFRASDKQRVLEARRASEKLLILSQLFTAENELLELENKIITQVKAKIGK
                     SQKEYFLNEQLKIIEKELGVGGEEDVELEEYDERLKKSKMSEEAREKAYRELNRLARM
                     AQLSPEATVSRTYIEWLLDLPWGKYTKDQEDLERAQKILDEDHFGLVKVKERIVEHLA
                     VLKLVKEMKGPILCLVGPPGVGKTSLARSIARTLKRRFVRVSLGGVRDEAEIRGHRRT
                     YIGSLPGKIIQSIKKAGSMNPVFLLDEIDKMNSDFRGDPASALLEVLDPEQNRTFADH
                     YLEVDFDLSRVMFITTANTTGGIPPPLLDRMELIRLPGYTEDEKRKIARGFLIPRQ"
     gene            1922..2254
                     /locus_tag="PLS90_06530"
     CDS             1922..2254
                     /locus_tag="PLS90_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02096.1"
                     /translation="MELPASIPDNERNNQAIEALLNRVRGLDKEARSRLSSMLDDEVD
                     LPYLDNMEREYAKAFRNIAPEHANVLRGRFVRALNEQRAFLIGMVRNALTEAELRDAQ
                     LHSKVIAE"
     gene            2465..2989
                     /locus_tag="PLS90_06535"
     CDS             2465..2989
                     /locus_tag="PLS90_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02097.1"
                     /translation="MTAFLRAAPFSPALRRPSALLRALWLAVALLHGWLIAERFVLGA
                     WGAPMDWLRALLCVAGIGYATLKFWRISTVFDQEPRRALIFAFILVLGHFWIAPPETQ
                     TAYLNHAEQTAASLLIVVPAVGLALLLALAARRLHRALPVPTPNAAGLPLQRPLPLRR
                     LKSHLFQRHLPVLF"
     gene            3102..4193
                     /gene="hemE"
                     /locus_tag="PLS90_06540"
     CDS             3102..4193
                     /gene="hemE"
                     /locus_tag="PLS90_06540"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HPK02098.1"
                     /translation="MTPASSAGSPGLASQPAAPGAPTPRERLLCALRGQPADRIPVWL
                     MRQAGRYLPQYHAVRARHDFLTLCKTPAAAAEVSIQPVEAIDSDAVIIFNDILIPLEH
                     CGARVAFDDHGPLIRNPVRRPEDLAGLAARPVADDEPIAATIAETRRRVGPDFPILGF
                     IGAPWTLAAYWVEGRMTRQFEHIGSLRFAAPELLLSLLERIADVAADYLRVQIAAGAD
                     AVQIFDTWGSILAADEYERFSGQWIRRIIERARPLGAPVIVYVNGCAPFLEQLNGLGA
                     DCLSIDWRLPLARARAALGPAIALQGNLDPLALLAGPEAVARTVEDLFRAFPAGPGHV
                     FNLGHGILPNTPVESARALIRAVKHHGRY"
     gene            4180..5589
                     /gene="hemN"
                     /locus_tag="PLS90_06545"
     CDS             4180..5589
                     /gene="hemN"
                     /locus_tag="PLS90_06545"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HPK02099.1"
                     /translation="MAVINETHRLRDLDRAFLERYNLVGPRYTSYPTAPHFHEGVDGA
                     AFRNHLLALTPAQRRRPLSIYIHLPFCIEHCSFCACNVIISPKMEQVSEPYVRMLERE
                     VELHAAVVDRDRPVIQFHWGGGTPTYLNVDQIRRVHAMVAGRFNLAADAEQSIEIHVS
                     WTSDEQLRTLAALGFNRVSLGVQDFNPRTQEAIRRRQTYERTRQVILLARELGFRGIN
                     VDLVYGLPYQTPATFGETIERIIDLRPDRLAVYNFAYLPGRLAHQRSIRPETLPSGEQ
                     KFQIFLEAHDRFSAAGYRYIGMDHFALPDDELSLAFDQGTMQRNFMGFTTRAGADLLG
                     IGVSAISHVTNCYAQNVKKPPAWERAIAAGEFPIERGLLLSRDDLIRQEVIAGLMCRD
                     HVNQHEIERHHGIVFDEYFAEEIDRLEPMVRDELLTIGPDTLDLTFLGRLFVRNIAMV
                     FDAYLKRPEGKQVVYSRTL"
     gene            5718..7157
                     /gene="hemG"
                     /locus_tag="PLS90_06550"
     CDS             5718..7157
                     /gene="hemG"
                     /locus_tag="PLS90_06550"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HPK02100.1"
                     /translation="MTNAPDADAADVRSTDVLILGGGISGLSLAHWLRRDNLDVHVLD
                     RNAQPGGVIGTLRKNDFLFERGPNTVLDKYDSFDDLLAGAGLDGELLRVPLRTQTRHI
                     WLNGRLHPVPMNPLAFLASPLLPVAGKLALLREPFVARRDDDETIADFVQRRLHASWV
                     SNLVTPMVSGIWAGDPARLSIAHAFPIMKALERRGGSLVRGAWLRARDRRRGAPSADA
                     PTTKARKHLVSFRDGLQRLPAALADRLADRYHGSVQVDAIAARADGGYEVRARGPAGA
                     LRWRARRLVIAAEADEAARWLAPLDAEAAAVLRAFPYNRLAVVGLGIRAGDARLPAGF
                     GFLVPRGQGVRILGAIINSNFLPGRAPAGCAVLHVFIGGELDPAAADLAEDELLGLAR
                     RDLRRTVDWRGEPLALHIERWPRAIPQYDLRHSERMRQIAAAEARHPGLHLVGNWRGG
                     VSIPDRVQYNRELAGRIVAAAPAAAPARP"
     gene            7189..8433
                     /gene="hemA"
                     /locus_tag="PLS90_06555"
     CDS             7189..8433
                     /gene="hemA"
                     /locus_tag="PLS90_06555"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPK02101.1"
                     /translation="MSPTSPMPPFESHTIEPPRLGPRRDELCALSLNHRRATVALRER
                     FALSRDDCAALVRRLLGGAVAREALVLSTCNRTEIYACGDRGALADSLRRVFEELAGP
                     EGHLPLPLELHEGEAAARHLFAVAAGLDSQILGENQIKQQVRDAVDVSRRAGGLGREL
                     NRLVEAVFRASKRIRTETGLNAGTLSIGRAAVLKAEAVLGTLAGKVCVVIGAGKIAGL
                     AARALAERRPARLCIVNRTPDNAAALARETRAEAHGLDALAPLLAEADFVLGAAWAPE
                     FILRADAWPAAPPARPRCLVDAAVPRVLDPDLARRPGVTLLDLDHLDDLIAENRARRA
                     GAARDAQAVIEQELARWRVARAHARLAPAFNDLRARFERAWQAECAVAGDDPRRRLRD
                     RRRLLLRELHRAIERLKAQVLD"
     gene            8684..9226
                     /locus_tag="PLS90_06560"
     CDS             8684..9226
                     /locus_tag="PLS90_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK02102.1"
                     /translation="MDPSADPALRELVLLVLNTGERIGEIRALRWRDYDESRGLLRLV
                     ATKLAARRRKAKERFIPVNAALKNLLAEMRKRDKPGPNDPIIRLSPNNMKRKFDGACR
                     LAGIGHARLYDLRHSALSHLAASGTPLHVLKSIAGHASVRTTEKYMHLLPGQAQRATE
                     ALNFGAKGEKAEVLEVADAG"
     gene            9636..12893
                     /locus_tag="PLS90_06565"
     CDS             9636..12893
                     /locus_tag="PLS90_06565"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HPK02103.1"
                     /translation="MSSKLVFETCAPRPDVLLGQTRDDHFMADLAKVVNRTALADYGD
                     PATFFSRTHPTRGLRELLKAVCKRLSPQGGEVASIIRLHTQYGGGKTHGLIALTHAVR
                     GMQRVESPEEFVDPALLPKEGNVRVAALDGENADPANGLTLETDLRAYSIWGEMAYQL
                     AGRDGYERVRESDRNHIAPGAETIRELFGGEPTLIMLDEISVYLRKVERAHPGAAAQF
                     TAFIHALIKAVASSPQAALVVTLAIGKDHQTPDAYQGEQTRALEAFEEAEKVVSRGAT
                     QLNPTEEDETVDVLRRRLFEQVDMSGVDDLVQEFAALWDKNRELLPEGITSVGLRELF
                     RKSYPFHPELLTLLTEKTATLSTFQRTRGMLRLLARTVHSLWEQQPADALVIHPHHIA
                     LGSSAIREEILVRLEQQDYTPPLKADVEAVTSDEDRALAQDLDARHYPSQAPLTTYIA
                     RTVFLHTLAYHNDLKGVQPDRLRYSVCSPAIEPAFVEQARERFVKESLYLDDRPGAPM
                     RFMVEANLEQMIRRQMKEIEAGEVRADLNVRIKHLFGMSNGPFNLCPFPAGAYEVPDE
                     ANDERPWLVVLGYEEYALGSDPSTVPPIVEEVFQFKGHDQKFRIFRNNLVFVVADARQ
                     VENMRTNVRRALALKEMQKPEIKSGLADYQQKRLESQSHESAMKVAEAILHCYRHLFY
                     PSSIPMQGSKQPLAHSVIEFAQASASPGNGQLHIDRILRQQTKILGVGDPPPAPAFVR
                     DKTALKTKGEISTLELRNEFRRAPNLQMLKGDEILVNCIRQGIETEVFIYREGDQVWG
                     KGDPTPTIQISENAYVHTLLDAKAKRLWPRAEPLELQFETKPTVVKAGEPATLGLTIS
                     GGTPPYQIASEIKEFAASKTEQTTFYAQVTPPASTSYAVEVVDARKKRRSATVEVRVA
                     VDGETLPPPPPVPPPPPPEPPKPRSFSCEGPLGQALTDLWEQVRKAGVQRIGTLLIKF
                     YSQSDAWKVHQAILALRNVEVSCQIAAEFESEDSDELTVAFRGKPEKANSLRGFLETQ
                     ARSCEESKIESAYTLRFPDGLQTDEATTEKLRKDLICYGAGAAWVEAHAAPGGEA"
     gene            12896..13366
                     /locus_tag="PLS90_06570"
     CDS             12896..13366
                     /locus_tag="PLS90_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02104.1"
                     /translation="MARQTRKAAQPAPEGPHFELRLKAPSRRDWRLEIWQLPSIATPR
                     LREPEFVGALKGTPLELVEHRLLKRLSRARIDLGPISAGRERKWSIDEDLALHLGLLF
                     RALAPMHNLGRIRQVADGIDEMSREEAGYWLGMTMQRKYPRRILAALRMVLTEP"
     gene            13466..>13665
                     /locus_tag="PLS90_06575"
     CDS             13466..>13665
                     /locus_tag="PLS90_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HPK02105.1"
                     /translation="MSIALGDLELVDLRDAWQSESQQFTPWLAQPANLAKLSAAVGLE
                     LEYQDREVAVGPFYADILARDTA"
     assembly_gap    13666..13765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13766..14199
                     /locus_tag="PLS90_06580"
     CDS             <13766..14199
                     /locus_tag="PLS90_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02106.1"
                     /translation="RYLDTLPKHPDYFTSRGFECFEEETTDPDRVVIFRKHMKLSGRK
                     VYLDLCLRYQVYISDIPSIRTSDNFDYEFMAIYLPVVQRDGDPEPRELGGSGEIGRWV
                     IDPQRYSDVRTLTRLLVSGSAEDNLWEEYEDEDDEEDDEDL"
     gene            14196..14954
                     /locus_tag="PLS90_06585"
     CDS             14196..14954
                     /locus_tag="PLS90_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02107.1"
                     /translation="MTSPRQLSVRQVIDLFHAGPGMARRMGQEAAMRAQAPEALACLE
                     EIIERLRAVGCDGLELLRSDGRLWSVLNLYEIWKGMMRQMGVSDPDPAQPADEMTHLD
                     EIIQRLDAVGCDGLKLLRTDPALRQKLAGYAAWQEARLTGGSVPQRLTFRIYGSPHHR
                     QAEGMLRLLKQMQASRRRVLLELTEGELRLTCGELSLCLPATFEQAGKRFVYTDFLPL
                     LMQALARDELVDCTIEGEYYTVNRAWFRPKQATG"
     gene            15008..15850
                     /locus_tag="PLS90_06590"
     CDS             15008..15850
                     /locus_tag="PLS90_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02108.1"
                     /translation="MEVQSIEQLGRELLQCQAELSSICQQGLNEQNSAAVAGTMVKYL
                     KLKREIRTAFLKEENSVVSIPNPEVRNLVRRVLRNEKTAADEFCREIFEQAPSEFDIE
                     SHLSGQPVFQDEPEPIPLLNDDWIDEAGYLSGLFEIGALVLDFDPLPSFLRDYVSQAR
                     RCYGHGFYLAVISLSRVILEICLRDYRRRTLEESRRAPMPESEANGGSLFELIDEVFD
                     RDKDRRAAHRVRIRMNKVAHGSFYGSDITREDAYQVFADTIHLVHLLYERAHNQQQER
                     EQDS"
BASE COUNT         2718 a         5607 c         4798 g         2685 t
ORIGIN      
        1 ctggcgcggg atgagaaagc cgcgggcgat cttgcgcttc tcatcctccg tgtagccggg
       61 cagccggatc agctccatcc ggtcgagcag cggcggcgga atgccccccg tcgtgttggc
      121 cgtcgtgatg aacatcacgc gcgacagatc gaagtcgacc tccaggtagt gatcggcgaa
      181 cgtccggttc tgttccggat cgagcacctc cagcagcgcc gacgccggat ccccccggaa
      241 atcgctgttc atcttgtcga tttcatcgag cagaaacacc ggattcatcg agccggcttt
      301 cttgatgctc tgaatgatct tgcccggcag cgatccaata tacgtgcgcc ggtggccgcg
      361 gatctcggct tcgtcgcgca ccccgcccag gctcacccgc acgaaccgcc gcttcagcgt
      421 ccgcgcgatc gaccgcgcca gcgacgtctt gcccactccc ggcggcccca ccaggcacag
      481 gatcggcccc ttcatctcct tgaccagctt cagcaccgcc aggtgctcga cgatccgctc
      541 cttcaccttc accagcccga agtggtcttc gtccagaatc ttctgcgccc gctccagatc
      601 ttcctggtcc ttggtgtact tgccccacgg caggtcgagc agccattcga tatacgtgcg
      661 gctgacggtc gcctccgggc tgagctgcgc catccgcgcc agccggttca gttcccggta
      721 ggccttctcg cgcgcctcct cgctcatctt gctcttcttg agccgctcgt cgtattcctc
      781 cagctcgacg tcctcctcgc cccccacgcc gagttccttc tcgatgatct tcagttgctc
      841 gttgaggaaa tactccttct gcgacttgcc gatcttcgcc ttgacctgcg tgatgatctt
      901 gttctccagc tcgagcagct cgttctcggc cgtgaacaac tgcgacagga tcagcagctt
      961 ctcgctggcg cgccgcgcct ccagcacccg ctgcttgtcc gacgcgcgga aatggcagta
     1021 gttggtgatc gcgttggcca gcggcacggc atcgtccaga ctgcggatcg agacggccag
     1081 atcctccggc accttgtccg aaagctgcac gtatgtctcg aactgcttct ccacggtccg
     1141 gcgcagcgcc tcgatctcct tgccccgttc ctcgaccgca tggatctcct cgaccagcgc
     1201 cgtcatgtag gagggttcgg acgtggcccg cacgatccgc gccgggtagc gcccctcgac
     1261 cagaatcttc gccgacccgt cgggcagccg cagcacctgc acgatccccg ccacgcagcc
     1321 cacttcgtac agatcctcca gacccgggtg ctcctcctcg acgttcttct gcgtcaccag
     1381 aaacaccgcc ttatcctggg ccagcgcgcc ctcgagcgcc tggagcgacc ggtgccgcac
     1441 gatgaacagc ggcgtgatac tgttgggaaa aaccacgatt tccttcagcg ggataattgg
     1501 cagaagcaac gagctcatca tgatgatcct ggcggcgggt ggatgagggg gtccgagatg
     1561 cggctcccac gcgaagccag gaccggggaa aggcgccgcc tcgagacccc ggcggaacgc
     1621 ctcccgtggc cggcatcggg ccggaacagg cgccagcgaa attttatatc atagcttgca
     1681 gccggtttca gcaggtcatt gcaaccggct gcccaacccg cccgtcgatg gaccctcctc
     1741 gttcacgcca aacattgatt tatcatgctt tatctgctcg cgcacggccg tttggagcat
     1801 ggccggatat ttgctttatc ccccttcgcg cagccaggat agccagccgc cgggccggat
     1861 tgcccgcgta actttcaacc cgcacttgga aacccaagtc atttgcccaa gggagcctca
     1921 catggaactg cccgccagca ttccggataa cgaacggaac aatcaggcca tcgaagccct
     1981 gctcaatcgc gtccgcggac tggacaagga ggcccgctcg cgactcagtt ccatgctcga
     2041 cgacgaggtc gatctgccct acctggacaa catggaacgc gaatacgcca aggctttccg
     2101 caacatcgcc cccgagcacg ccaacgtcct gcgcggccgc ttcgtccgcg ccttgaacga
     2161 acagcgcgcc ttcctcatcg gcatggtccg caacgccctg accgaggccg aactgcgcga
     2221 cgcccagttg cacagcaaag tcatcgccga ataatccggc ggccggcgaa cccggcccac
     2281 gagtcttgca ctccaccctc agagagcccg cccccccggc gggctctcct ctttcccccc
     2341 aaccttcgtc ccccgtcctc ggctctcgaa ttccccgcca tttttccctt gaaccccgcc
     2401 gccgccatgc tagactgaaa agtcgattcg gatgtccgaa ttagacccgc gtacccgaga
     2461 cccgatgacc gccttcctcc gcgcagcccc cttctccccc gccctccggc ggccctcggc
     2521 gctcctgcgc gccctctggc tcgccgtggc gctgctccac ggctggctca tcgccgagcg
     2581 gttcgtcctc ggcgcctggg gcgccccgat ggactggctg cgcgcgctcc tctgcgtcgc
     2641 cggcatcggc tacgccacgc tcaagttctg gcgcatctcc accgtcttcg accaggagcc
     2701 ccgccgggcg ctgatcttcg ccttcatcct ggtcctcggc cacttctgga tcgcgccccc
     2761 cgaaacacag accgcctatc tgaaccatgc cgaacagacg gcggcgtcgc tgctgatcgt
     2821 cgtgccggcg gtcggcctgg cgctgctgct ggcgctggcc gcgcgtcggc tccaccgcgc
     2881 gctgccggtc ccgactccga acgcggccgg tctccccctc cagcggccgc tgccgctccg
     2941 ccgcctcaag tctcatctct ttcaacgaca ccttcccgtc ctgttctgat cccatggggt
     3001 aggggggccg gaccaacggc cccggcgcgg gacgcgttcc cgcccgcacg gcgcgttcgc
     3061 ccgccgtgcg gaccaggacc ccaatgggac ggaatggtct gatgacgcct gcttcctccg
     3121 ccggatcacc cggccttgcg agccaaccgg ccgcccctgg cgccccgacg ccgcgcgagc
     3181 ggctcctgtg cgccctgcgc ggccagcccg ccgaccgcat cccggtctgg ctgatgcgcc
     3241 aggcgggccg ctacctgccg caataccacg ccgtccgcgc gcgacacgat ttcctgacgc
     3301 tctgcaaaac ccccgccgcg gcggccgaag tctcgatcca gccggtcgag gcgatcgact
     3361 ccgacgcggt catcatcttc aacgatatcc tgatcccgct ggagcactgc ggcgcccgcg
     3421 tcgccttcga cgatcacggc ccgctgatcc gcaatcccgt ccgccgcccc gaggatctgg
     3481 ccgggctcgc cgcgcgcccg gtggccgacg acgaaccgat cgccgccacc atcgccgaaa
     3541 cgcgccggcg cgtcgggccc gatttcccga tcctcggctt catcggcgcc ccctggacgc
     3601 tcgccgccta ctgggtcgaa gggcggatga cccgccagtt cgagcatatc ggctcgctgc
     3661 gcttcgccgc gccggagctg ctcctctccc tgctggaacg aatcgccgac gtcgccgccg
     3721 actacctccg cgttcagatc gcggccggag ccgatgccgt ccagatcttc gacacctggg
     3781 gctcgatcct cgccgccgat gagtatgagc gcttctcggg ccaatggatc cggcggatca
     3841 tcgagcgcgc gcggcccctc ggcgcgccgg tcatcgtgta tgtcaatggc tgcgccccct
     3901 ttctcgaaca actcaacggg ctgggcgccg attgcctgag catcgactgg cggctgccgc
     3961 tggcgcgcgc gcgcgccgcc ctcggccccg ccatcgccct ccagggcaac ctcgatccgc
     4021 tggcgctgct ggccggcccc gaggccgtcg cccgcaccgt cgaggacctc ttccgcgcct
     4081 tcccggccgg acccggccac gtcttcaacc tgggccacgg gatcctgccc aacacccccg
     4141 tcgagagcgc ccgcgccctg atccgggcgg tgaaacacca tggccgttat taacgaaact
     4201 caccgcctgc gcgacctgga ccgcgccttc ctcgagcgct acaacctcgt cgggccgcgc
     4261 tacacctcct atccgacggc cccccatttc catgagggcg tggatggggc ggccttccgc
     4321 aaccacctgc tggcgctgac gcccgcgcag cgccgccgcc cgctctcgat ctacatccac
     4381 ctgccgttct gcattgagca ctgctcgttc tgcgcctgca acgtcatcat ctcgccgaag
     4441 atggagcagg tcagcgaacc ctacgtgcgg atgctggagc gcgaggtcga gctccatgcc
     4501 gcggtcgtcg atcgcgaccg cccggtgatc cagttccact gggggggcgg cacgccgacc
     4561 tacctgaacg tcgaccagat ccgccgcgtc cacgcgatgg tggccggccg gttcaacctc
     4621 gccgccgacg ccgagcagtc gatcgagatc cacgtcagct ggacgagcga cgagcaactg
     4681 cggacgctcg ccgcgctcgg cttcaatcgc gtctcgctcg gcgtgcagga tttcaacccg
     4741 cgcacccagg aggccatccg ccgccggcag acctacgagc gcacccgcca ggtcattctg
     4801 ctcgcccgcg agctcggctt ccgcggcatc aacgtcgatc tggtctacgg cctgccgtat
     4861 cagacccccg cgacgttcgg cgagacgatc gagcggatca tcgacctgcg ccccgaccgc
     4921 ctggccgttt acaacttcgc ctacctgccc ggccggctcg cgcaccagcg ttcgatccgc
     4981 cccgagaccc tcccctccgg cgaacagaaa ttccagatct tcctcgaggc ccacgaccgc
     5041 ttctccgccg cgggttatcg ctacatcggc atggaccatt tcgcgctgcc cgacgacgaa
     5101 ttgtcattgg cttttgacca ggggacgatg cagcgcaatt tcatgggctt caccacccgc
     5161 gccggcgccg acctgctcgg catcggcgtc tcggcgatca gccatgtaac gaattgctac
     5221 gcgcagaacg tgaaaaaacc acccgcgtgg gagcgcgcga tcgcggccgg cgagtttccc
     5281 atcgagcgcg ggctgctcct cagccgcgac gacctgatcc gccaggaagt catcgccggc
     5341 ctcatgtgcc gcgatcacgt caatcaacac gagatcgagc gccatcacgg aattgtcttt
     5401 gatgaatact tcgcggagga aatcgatcgg ctggaaccga tggtgcggga cgaactgctg
     5461 acgatcggcc ccgatacgct ggatctgacc tttctcggcc gcctgttcgt gagaaacatc
     5521 gccatggtct tcgatgcgta tctgaaacgc cccgagggaa aacaggtcgt ctactcgcga
     5581 actttataag gaggatccgt atttgaattt gtttcgtatt ttgaaattcg aattgcgaat
     5641 tttcattaca acgacccgga acggaaacaa tcattcctga gatggctgcg atgacactgg
     5701 cgacgcgcca aattccaatg acaaatgccc ctgacgcgga cgcggccgat gtccgttcaa
     5761 ccgacgtgct catcctcggc ggcggcatct ccggcctctc gctggcccac tggctccgcc
     5821 gcgacaacct cgacgtgcac gtcctcgacc ggaatgcgca acccggcggc gtcatcggca
     5881 cgctccgcaa gaacgacttc ctcttcgagc gcgggcccaa caccgtcctc gacaagtacg
     5941 attcgttcga tgacctgctc gccggcgccg gcctcgacgg cgaattgctg cgcgtcccct
     6001 tgcgcacgca gacccgccac atctggctca acggccgcct gcatcccgtg ccgatgaatc
     6061 cgctggcgtt tctcgcttcg cccctgctcc ccgtcgcggg caagctcgcc cttttgcgcg
     6121 agccgttcgt cgcgcgccgc gacgacgacg aaacaatcgc cgacttcgtc cagcgccggc
     6181 ttcacgcaag ctgggtctcg aatctggtca cgccgatggt ctcgggcatc tgggccggcg
     6241 accccgcccg gctcagcatc gcccacgcgt ttccgatcat gaaggccctg gaacggcgcg
     6301 gcgggagcct cgtccgcggc gcctggctgc gcgcgcgcga ccgccgccgc ggcgccccct
     6361 ccgccgacgc ccccaccacc aaggcgcgca agcacctggt ctcgttccgc gacggcctgc
     6421 agcgcctccc cgcggccctc gccgaccgcc tcgccgaccg ctaccacggc tcggttcagg
     6481 tcgacgcgat cgccgcgcgc gccgacggcg gctacgaggt ccgggcgcgc ggcccggcgg
     6541 gcgccctccg ctggcgggcc cgccggctcg tcatcgccgc cgaggccgac gaagccgccc
     6601 gctggctcgc cccgctcgac gccgaagccg ccgccgtcct gcgcgccttc ccctataacc
     6661 gcctcgcggt cgtcggcctg ggcatccgcg ccggcgacgc gcgcctcccc gccgggttcg
     6721 gctttctcgt cccgcgcggc cagggggtgc gcatcctcgg cgcgatcatc aactcgaact
     6781 tcctgcccgg ccgcgccccc gccggctgcg ccgtcctgca cgtcttcatc ggcggcgagc
     6841 tggacccggc cgccgccgac ctcgccgagg atgagctgct cggcctcgcc cgacgcgacc
     6901 tgcgccggac ggtcgactgg cgcggcgagc ccctcgcgct ccacatcgag cgctggccgc
     6961 gcgccatccc gcaatacgac ttgcgccaca gcgaacggat gcgccagatc gccgccgccg
     7021 aagcgcgcca ccccggcctc cacctggtcg gcaactggcg cggcggcgtc tcgatccccg
     7081 atcgcgtgca atacaatcgc gagctggccg ggcggatcgt cgccgccgcg cccgccgccg
     7141 ccccggcgcg cccatgagtt cctccaatcc atcgcgggag tgattgactt gagtccgacc
     7201 agcccgatgc cccccttcga atcgcacacg atcgagccgc cgcggctcgg tccgcggcgc
     7261 gacgagctct gcgccctcag cctcaatcac cgccgcgcca ccgtcgccct gcgcgagcgc
     7321 ttcgccctca gccgcgacga ttgcgccgcg ctcgtccgcc ggctcctcgg cggcgccgtc
     7381 gcgcgcgagg cccttgtcct ctccacctgc aaccgcaccg agatctatgc ctgcggcgac
     7441 cgcggcgcgc tggccgactc cctgcgccgc gtcttcgaag agctcgccgg gcccgaaggc
     7501 catctccccc tccccctcga gctgcatgag ggcgaggccg ccgcccgcca cctgttcgcc
     7561 gtcgccgccg gtctcgactc gcagatcctc ggcgaaaacc agatcaagca gcaggtgcgc
     7621 gacgccgtcg acgtcagccg gcgcgccggc ggcctcggcc gcgagctcaa ccgccttgtc
     7681 gaggcggtct tccgcgccag caagcgcatc cggaccgaaa ccggcctcaa cgccgggacg
     7741 ctcagcatcg gccgcgccgc cgtcctcaag gccgaggccg tcctcggcac gctggccggc
     7801 aaggtctgcg tcgtcatcgg cgcgggcaag atcgccggcc tcgccgcgcg cgccctcgcc
     7861 gaacgccgcc ccgcccgcct ctgcatcgtc aaccgcacac ccgataacgc cgccgcgctc
     7921 gcgcgcgaaa cccgcgccga ggcgcacggc ctcgatgcgc tcgcccccct gctcgccgag
     7981 gccgacttcg tcctcggcgc cgcctgggcg cccgagttca tcctccgcgc cgacgcctgg
     8041 cccgccgccc cgccggcccg cccccgctgc ctcgtcgatg ccgccgtccc gcgcgtcctc
     8101 gatcccgacc tcgcccggcg gccgggcgtc accctgctcg acctggatca tctggacgac
     8161 ctgatcgccg agaaccgcgc ccgccgcgcc ggcgccgccc gcgacgccca ggccgtcatc
     8221 gagcaggagc tcgcccgctg gcgcgtcgcc cgcgcgcatg cccgcctggc gccggcgttc
     8281 aacgacctgc gcgcccggtt cgagcgcgcc tggcaagccg aatgcgccgt ggccggcgac
     8341 gatccccgcc gccgcctccg cgaccgccgc cgcctcctcc tgcgcgagct ccaccgcgcc
     8401 atcgaacgcc tcaaggccca ggtgctggac tgaccggcca cttgccaggc aaaccggcgc
     8461 cgccgatggc cccgctttgc cccattccca atcgaccggc gtcaagccag cgatcctgga
     8521 gtgcagtgcc agagccttgc cacgccatgc gccggcaccg ctttggatcc gaggatccgt
     8581 cggcgttttc ttatttgagt tgcagccttc gaattgctcg gatttcggat tcgacgcttc
     8641 atctcggtca gccgcccgcc agcccgcgcc agcgggcttt ccattggacc cctcagcgga
     8701 cccggcgctg cgcgagctgg tcttactggt cctgaacacg ggggagcgca tcggcgaaat
     8761 tcgggcgctg cgctggcgcg attacgacga gagccgggga ctgcttcggc ttgtcgctac
     8821 gaagctggcc gccaggcgtc ggaaggccaa agagcggttc atcccggtca acgcggcgct
     8881 taagaacctt ctcgcggaaa tgcgcaagcg ggacaagccg gggccgaacg acccgatcat
     8941 tcggctgtca cctaacaaca tgaagcgcaa attcgacggg gcctgccggc tggcagggat
     9001 cgggcacgcg cggctttacg atctccgaca ctccgcactc tcgcatctgg ccgccagcgg
     9061 aacaccgctt cacgtcctca agtccatcgc cggccatgcc tcggtgagga ccaccgagaa
     9121 gtacatgcac ctgctgcccg gccaggccca gcgcgcgacc gaggccctga acttcggggc
     9181 caagggcgaa aaggcggagg tgctggaggt ggctgacgcc ggctgacgag gtcgatgggc
     9241 gggctctacg gccggagaaa aaagctggct ggtgagtgat tccatctacc gcctcctttc
     9301 tcctggcccg tctcaaggtc ggaaatgctt aggctttgct taggggggac ctacctgcga
     9361 cgacacccca aactggaacg cctcgatgtc aagcggggat ggcaagaaaa tcgggggagc
     9421 agagtcctca cgctggatcg atgcatgtca atcccgagga ccgaagtggg ccatctcgcg
     9481 ctccgagaag ggctggtgct ccccctcgtt cctgcatctg aacccgcaga ggtaatctga
     9541 gaatccaggt tgcgaattga cacttccagc cagtcctgtc acatttggac acgctgaatt
     9601 gaaaacctgt cgctcgcgtg cgaggggggg ttgcgttgag ttcgaaattg gtttttgaaa
     9661 cttgcgcccc gcggccggat gtcctgctgg ggcagacccg cgacgaccat ttcatggcgg
     9721 acctggccaa agtggtcaac agaaccgcac tggccgatta tggcgatcct gcgaccttct
     9781 tctcccgcac ccaccccacc cgcggtctcc gcgaactgct taaggccgtc tgcaagcggc
     9841 tgagcccgca agggggcgaa gtcgcctcta tcatccgtct gcatacccag tacggcggcg
     9901 gcaagacgca cggcctgatc gccttgaccc atgctgttcg agggatgcag agggtcgagt
     9961 ccccggaaga gttcgtcgat ccggccttgc tgcccaagga gggcaacgtg cgggtggcgg
    10021 cgttggatgg cgagaatgcc gatcccgcca atggactgac gcttgaaacg gacttaagag
    10081 cctattcgat ctggggcgag atggcatacc agcttgccgg gcgcgacggt tacgaacgtg
    10141 tacgagaaag tgatcggaac catatcgcgc ccggcgccga aaccatccgc gagctcttcg
    10201 gcggcgagcc gaccctgatc atgctcgatg agatcagtgt ctacctgcga aaggtggaaa
    10261 gagcccatcc gggcgccgcc gcccagttca cagcgttcat tcatgcgctg atcaaggctg
    10321 ttgcgtcatc gccccaggcc gcgctggtgg taaccctggc gatcggcaag gatcatcaga
    10381 cgcccgacgc ctatcagggc gagcagaccc gcgcgctgga agctttcgag gaagcggaaa
    10441 aggtcgtctc gcgaggcgcc acccagctca atccgactga ggaagatgag acggtcgatg
    10501 ttctccggcg gcgtctgttt gaacaggtcg acatgtcggg tgttgacgat ttggtccagg
    10561 agtttgccgc actgtgggac aagaatcggg aactgctgcc cgaagggatc acgagcgttg
    10621 gcctgcgcga gctgttccgc aagagctatc cgtttcatcc cgaactgctg acgctcctga
    10681 ccgaaaaaac ggccaccctc agcacatttc agcggacgcg gggcatgttg cgcctactcg
    10741 cgcgcaccgt gcactccctc tgggagcaac agcccgccga tgccctcgtt attcatcccc
    10801 atcacatcgc cttggggtcg tcggcgatcc gcgaggagat cctcgtccgc ctcgaacagc
    10861 aggactacac ccccccgctt aaagccgatg tggaggcggt gacgagcgac gaggatcggg
    10921 cgctggcgca agatctcgat gcccggcact atcccagcca ggcgccgctg acgacctaca
    10981 tcgcccggac ggtcttcctg cacacgctgg cctatcacaa cgacctgaaa ggcgtccagc
    11041 ccgaccggct ccgctactcg gtctgttcac ccgccatcga accggccttc gtcgagcagg
    11101 cgcgcgaacg attcgtgaag gaatccttgt atctggatga ccggccggga gctccgatgc
    11161 gcttcatggt cgaggccaat ctggagcaga tgatccggcg ccagatgaag gagatcgagg
    11221 cgggagaggt tcgcgccgat ctgaatgtgc ggatcaaaca cctcttcggc atgtccaacg
    11281 gcccgttcaa cctctgccca ttcccggccg gcgcctacga agtgcccgac gaagccaatg
    11341 acgagcggcc gtggctggtc gtgctgggat acgaggaata tgccctgggc agcgacccca
    11401 gcacggtgcc gcccatcgtc gaggaagttt ttcagttcaa ggggcatgat cagaagtttc
    11461 gcattttcag gaacaacctg gtctttgttg tcgccgatgc gcgtcaggtc gagaacatgc
    11521 ggaccaacgt tcggcgcgcg ctcgccctca aggagatgca gaagccggag atcaagtcag
    11581 ggcttgccga ctatcagcag aagcgccttg agtcgcaatc gcatgaatca gcgatgaaag
    11641 ttgccgaggc catcctgcat tgttaccggc atctcttcta cccttccagc atccccatgc
    11701 agggcagcaa gcagcctctg gcgcattccg tgatcgagtt tgcccaggcg agcgcatcgc
    11761 ccggcaatgg ccagctccac atcgatcgca tcctgcggca acagaccaag atcctcggtg
    11821 tcggggatcc gccgcctgcc ccggcgttcg tccgcgacaa gacagccctg aagacgaagg
    11881 gcgaaatctc gaccctggaa ctgcgcaacg aattccgccg ggcccccaac cttcagatgc
    11941 tcaaagggga tgaaatcctc gtcaactgca tccggcaggg tatcgagacg gaagtcttca
    12001 tctatcgcga aggagatcag gtctggggca agggcgaccc gacgccgacc atccagatca
    12061 gcgaaaatgc ctacgtgcat acgttgcttg acgccaaagc caaacgcctc tggccgcgtg
    12121 ccgaaccact ggagctgcag tttgagacca aaccgacagt cgtcaaggct ggcgagccgg
    12181 ccacgctggg cttaaccatc tccggaggga cgcccccgta ccagatcgct tcggagatca
    12241 aggagttcgc cgcaagcaag accgagcaga cgacattcta cgcgcaggtg accccgcccg
    12301 cttccaccag ctatgccgtg gaggtcgtcg atgcccgcaa gaaacgccgc tccgccaccg
    12361 tcgaggtccg agtcgcggtc gacggcgaga ccctgccgcc gcccccaccc gtgccgcccc
    12421 ccccgccgcc ggaaccgccg aagccaaggt cgttcagctg cgaggggccg ctggggcagg
    12481 cgcttacgga tctgtgggag caggtccgca aagccggggt gcagcgcatc ggaacattgc
    12541 tgatcaagtt ctattcccaa tccgacgcct ggaaggtgca ccaggcgatc ctggccctgc
    12601 gaaatgtgga agtctcctgc cagattgcgg cggaatttga atcggaagat tccgatgagc
    12661 tgacagtcgc cttccgcgga aagccggaaa aggccaactc cttgcgcggc ttcctggaaa
    12721 cccaggcgcg cagctgtgag gagtcgaaaa tagagtctgc atacaccctg cgcttccccg
    12781 atggtctgca gacggacgag gcgaccacgg agaagttgcg caaggatctg atctgttacg
    12841 gcgccggcgc cgcctgggtt gaagcgcacg ccgcgcccgg cggggaggcc tgatcatggc
    12901 caggcagacc cgcaaagccg cgcagcccgc gccggagggg ccccactttg aattgcggct
    12961 caaggcgccc agccggcgcg actggcggtt ggaaatctgg cagctcccgt cgatcgccac
    13021 cccgcgcctg cgcgaaccgg aatttgtcgg ggcgctcaag ggcacgccgc tcgagcttgt
    13081 cgagcatcgg ctgctcaagc ggctttcacg cgcgcgcatc gacctcggcc ccatcagcgc
    13141 cggccgcgag cgcaagtggt cgatcgacga ggatctggcc ctgcatctgg gcctgctgtt
    13201 ccgggcgctg gcgccgatgc acaacctggg ccgcatccgg caggtggccg acggcatcga
    13261 cgagatgagc cgcgaggagg cgggctattg gctggggatg accatgcagc ggaaatatcc
    13321 gcggcgcatc ctggcggcgc tgcggatggt cctgacggag ccgtgacaat cgttatgata
    13381 ctgaacggca ggaaagtatc ataagccgac cactcattac actttagtat cacaagagcc
    13441 gtggccgttt tggagggatt aatcaatgag cattgccctt ggcgatctgg agttggtcga
    13501 tctgcgcgat gcctggcaga gcgagtcgca gcagttcacg ccctggctgg cacaaccggc
    13561 gaacctcgcc aaactcagtg ccgccgtagg gctggagttg gagtatcagg atcgggaggt
    13621 ggcggtcggc ccgttctatg ccgacatcct tgcccgcgat accgcnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnntcagg tatctggaca ccctgcccaa gcaccctgac
    13801 tacttcacca gcagggggtt cgagtgcttt gaggaggaaa caacagaccc cgaccgggtc
    13861 gtgatcttcc gcaagcacat gaagctgtca ggccggaagg tctacctcga tctctgcctg
    13921 cggtatcagg tctatatttc ggacattccg tcgatccgca cgagcgacaa ctttgactac
    13981 gagttcatgg ccatctacct ccctgtcgtc cagcgcgacg gggatcccga gccgagggag
    14041 cttggcggta gcggggaaat cggacgctgg gtcatcgatc ctcagcgata ttcagacgtg
    14101 cgaaccctca cccggctgct ggtctccggg agcgccgagg acaacctgtg ggaggagtat
    14161 gaggacgagg atgacgagga ggacgatgag gacctgtgac ttccccccgt cagctcagcg
    14221 tccgccaggt gattgacctg ttccatgcgg ggccggggat ggcgcggcgg atggggcagg
    14281 aggcggcgat gcgggcccag gcgcccgagg cgctggcctg cctggaggag atcatcgagc
    14341 ggctgcgggc ggtcggctgc gacggcctgg agctgctgcg ttccgacggg cgcctctggt
    14401 ccgtgctgaa cctgtatgag atctggaagg ggatgatgcg gcagatgggc gtgtcggacc
    14461 cggacccagc ccagccagcc gatgagatga cccatctgga cgagattatc cagcggctcg
    14521 acgcggtcgg ctgcgacggg ctgaagctgc tgcgcaccga ccccgccctg cgccagaagc
    14581 tcgcggggta tgcggcctgg caggaggcac ggctgaccgg cggcagcgtg ccgcagcggc
    14641 tgaccttccg catctacggc tcgccgcacc accgccaggc cgaggggatg ctcaggctgc
    14701 tcaagcagat gcaggcgagc cgccggcgcg tcctgctgga actgaccgaa ggggagctgc
    14761 gtctgacatg cggggaactt tccctctgcc tgccggcgac attcgagcag gccggcaagc
    14821 ggttcgtcta taccgacttc ctgcccctgc tgatgcaggc cttggcgcgc gatgagctgg
    14881 tcgattgcac gattgagggg gaatattaca cggtcaaccg ggcatggttc cgcccgaagc
    14941 aggcaacagg atagggggtt ttgcccagtg agagacggat tagccgccaa ggctgaagga
    15001 gctcaagatg gaggtacaaa gtatcgagca actagggcgg gagctgctcc agtgccaggc
    15061 ggagctttcc agcatctgcc agcaggggtt gaacgagcag aactcggctg ccgttgctgg
    15121 aaccatggtg aaatacctca aactcaagcg ggaaatccga acagctttcc tgaaggagga
    15181 gaattccgtc gtctccatcc cgaatcccga ggtgcggaac ctggtgcgcc gggtgctgcg
    15241 gaacgagaag acggcggctg acgaattctg ccgtgagatc ttcgaacagg ccccgtctga
    15301 gttcgacatc gagtcgcact tgagcgggca gccggtcttt caggacgagc cggaacctat
    15361 cccgctgctg aatgatgatt ggatagacga agcgggatac ctttccgggt tatttgagat
    15421 cggcgccctt gtgctcgatt tcgaccccct gccatcgttc ctgcgggact atgtcagcca
    15481 ggcccggaga tgctatggac atggcttcta cctcgcggtc atcagtctgt cgcgggtaat
    15541 cctggagatc tgcctgcgtg attaccgacg gagaaccctg gaagagagcc ggcgggcccc
    15601 gatgccggag tctgaagcta atggggggtc gctcttcgaa ctcattgacg aagtttttga
    15661 cagggacaaa gatcgcagag cggcccacag ggtgagaatc aggatgaaca aggtggccca
    15721 tggcagcttc tatggctcag atatcaccag ggaagacgcg tatcaggtat tcgccgatac
    15781 gatccacctg gtccatttgc tctacgagcg ggcacataat caacaacagg aacgggagca
    15841 ggattcgtga ccgacctcga gttaaatacg ggcatgcaga cgcggcggtt gatcgaagag
    15901 tggatgcc
//
LOCUS       DAOPCO010000064        15881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3759_length_15881_cov_5.361304, whole genome shotgun sequence.
ACCESSION   DAOPCO010000064 DAOPCO010000000
VERSION     DAOPCO010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3759_length_15881_cov_5.361304"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLS90_06595"
     CDS             complement(<1..483)
                     /locus_tag="PLS90_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02109.1"
                     /translation="MPTVTSLSRVARLAIHNIERIFDRDRDDLPYFGVSLLAARPHLY
                     HRHVFDVPHLPGRALDALYLAHQVFGLEAQSEVVELWRRWHLAAFDEVSGLNGFPTEQ
                     GGRTIILHNLREGLAGLAALWAWHEDQAARRVARRMIERLDEPIRRPAVEGKYGSCRT
                     V"
     gene            complement(649..1239)
                     /locus_tag="PLS90_06600"
     CDS             complement(649..1239)
                     /locus_tag="PLS90_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02110.1"
                     /translation="MTHRRDAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVSRNM
                     ADMRSLAVGLEAYHVDSAHYPTGLIDVNAPGIFRSYLRRLIPLTTPVAYVTSIPDDVF
                     NLFGEPGAVPPSEYKTFDYFLITDLLAASLVEFNDQRGVRINAVYQMRGLGPDRDNDR
                     FSGAREGKGPIPYDPTNGTISDGDMKYFGPGGLFHL"
     gene            complement(1607..2635)
                     /locus_tag="PLS90_06605"
     CDS             complement(1607..2635)
                     /locus_tag="PLS90_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK02111.1"
                     /translation="MPCSLKDIALEANVSINTVSRALRGMPGVKHETRRRVQSIARRL
                     GYRPNTAARLLVLKRSHSIGVLVPDLNVPFFAETTIAIQDCLQSSHYYAYIVNTHNDP
                     DRLHEGMELLMEHRIEGLISITRITDKAAEMVDRHGLPHVSLIMSGGAMPPHFVGYDD
                     YAGGRLAARHLVELGHRRIAYYTGHWERDLKPPRYAGFVDEIAASGVDLEVSRYPDTE
                     QNTIRSGYEDSARLVRDILKGGVRAVMCNCDAVALGLMHRLNETGLRIPEDVSVIGYD
                     DIEMAEFCIPPLTTVAQKAGRLGELGSKLMLQLLGEKLWQDARHQILLEPELKVRRST
                     AAPRKTPK"
     gene            2988..4085
                     /gene="ftsW"
                     /locus_tag="PLS90_06610"
     CDS             2988..4085
                     /gene="ftsW"
                     /locus_tag="PLS90_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPK02112.1"
                     /translation="MRTLNTPLLLFMLTLALVTCGIVAVYSASASSAYLTKQLLFAGI
                     GLAAMLFCYAIDYHVLKRASFWLMLVAFGLCCLVLVPGIGNEVKGARRWLALGPISIQ
                     PSELAKLALIIYMAKMLSERRAVIKSFFSGVFPALVVTGAFAAIIVIEPDFGAAFVLC
                     LITFGMWLAAEMRWFHLVGLVLAAVPAALVAFLSEPYRAKRLIAFMFRDKETLLGAGW
                     QLHQSLIAIGSGGLWGLGLGESRQKFHYLSEAHTDFIFAIMAEELGFVAMSCIVGMFL
                     LITVLGWKVAWRTTDLFGSLLATGVTLMVFIGASINMCVVLGLLPTKGLVLPFISAGG
                     SSLIVCMAAMGILMNVAASLYHHQGARAVTA"
     gene            4312..5463
                     /gene="murG"
                     /locus_tag="PLS90_06615"
     CDS             4312..5463
                     /gene="murG"
                     /locus_tag="PLS90_06615"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPK02113.1"
                     /translation="MTDAAGPRLLLAAGGTGGHIMPAVATAEALRELAPDVQIDFVCG
                     HRPSEWQIYRRLGIEPWTLPLTFNRPGLMNRMRFLREFLAALREARQRLRAHPCDLVV
                     GFGSYMSVPPVLAARLAGIPAVVVEQDLRPGMANRLLAPLARKVAAAFETSAGFPRGK
                     TTVVGNPIRADIIRPVDRVEARRFFRLSPHRLVCFCVGGSQGARGINQLLLDLLRRQH
                     AGGEETAGAAAQWQLLWSTGPAHFDHVVRALREMGVDPAEHSINPYIDKMALAYAAAD
                     LVLCRTGALTLAELTAVGRPAILVPLPSAAGGHQAANARRLVEAGAAELILETDPAAA
                     ERLETLLARWVEQPGWLAEMAHAARKLGRPHAARDLARMLLDCLPSRGD"
     gene            5517..6935
                     /gene="murC"
                     /locus_tag="PLS90_06620"
     CDS             5517..6935
                     /gene="murC"
                     /locus_tag="PLS90_06620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPK02114.1"
                     /translation="MDDLRQFQRIHFIGIGGVGMSAIATVLLHHGFTVSGSDRAKSAA
                     TLRLQAAGARIFHGHEAANIDDADLVVVSTAIRPDNPELVAAREQERTIWHRSQALAC
                     IMRMGRSIAISGTHGKTTTTALLGLMLTEAGCDPTVVIGGSLPNFGGANARCGNGPWV
                     VVEADESDASFLRMDPDRILISNIEADHLDFYSDQAAVEAAFKKFLGRLRDGGRMILC
                     IDDPALAALARIDEHHAITYALADPAAEMHAAAVETIDGGLGSRFTPVWRGQALDPVE
                     LHVPGRHNVQNALGALACALEIGADYEGLRRGLAEFVGAGRRFQFKGVCGDIAVYDDY
                     AHHPTEIAATLAAARAQIESGRAARLVAVFQPHRFTRTQHLADQFGDALDVADVAVVT
                     EVYSAGENPIAGVSGRLIHDRLLALGHRSAQYLPTLDEVRAGLAEIVMPGDLVLTLGA
                     GNIWQVGEGLLAELRERSAELA"
     gene            6978..7640
                     /locus_tag="PLS90_06625"
     CDS             6978..7640
                     /locus_tag="PLS90_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02115.1"
                     /translation="MARAAARLTRAIALILAFLPGLVGCGREPVETAIARLEERPAIY
                     ADHEVRVRGTVTAHLGTKNPGEPMRYVLRDRLSEREILVVPALERKDELPGLGAEVAV
                     VGRVAPAAQDAGGAASGAALVLREKSAGGNLTDEPLVFWGAMAVGLALLGAVGWLLLS
                     MMHSPRHGARYARTGHVNGLGRRTLETAAPAPAAPRPPAGEISRKPADAPEDDSSVGD
                     MI"
     gene            7903..8727
                     /gene="lpxA"
                     /locus_tag="PLS90_06630"
     CDS             7903..8727
                     /gene="lpxA"
                     /locus_tag="PLS90_06630"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPK02116.1"
                     /translation="MAQIHPTAVVDPSARLADDVTVGPYTIIEADVTVGAGSRIGAHV
                     TLGERLRLGCRVRVFNYACLGTASQDLKHRGEISRAEIGDDCIIREFVTVNRGTREGS
                     VTRVGRGVALLAYSHVAHECVIEDGAILVNGATLGGEVHIGRRATLGGLVGIHQYCRV
                     GDYAFVGANSKVTQDVPPFLLVDGHPARPFGPNVVGLQRNGFSEEQIREIHRLYQIVF
                     DRSRGLNDNLAYIREKHGASPIARTILEFCRDGSRGIVRPRQRRRMAMIPGDLQFT"
     gene            complement(9167..11134)
                     /locus_tag="PLS90_06635"
     CDS             complement(9167..11134)
                     /locus_tag="PLS90_06635"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK02117.1"
                     /translation="MILIRPSKYDDDGYIMRFFRGVLPSNTLAALAGLTREAAATGAL
                     GRLKVETLMLDEYVQPVDVRRLARRARRPDTRAVVALCGVQTNQFPRAADLAREFRAA
                     GMQVMIGGFHVSGAIALSDYAAGGPLPPECQALLDDGVTLVKGEVENCWGDLLRDALH
                     HRLRPFYDIADRPDIWSAAIPVVDPRLMKRYAYPFMGTIDAGRGCPFGCSFCTIINVQ
                     GRQMRCRNPEAIKERVRANAAHDIDYYFFTDDNFSRNPHWEAIFDALIALRREEGLQF
                     QFMMQVDVLAYRIRRFMSKAAEAGCSQVFIGMESINTDNLDAAGKKQNLAVDYQEMFD
                     AWHAFGIACHVGYIIGFPHDTVESVRADVRRLKELGADQASFFMLTPLPGSADHVNMA
                     RRGEWMDPDYNRFDSFHPVTEHPRMSREEWFEAYREAWREFYSLDNMKQILARANQRT
                     YWGLFKNFMWYRYAAFVEETHPMICGFVRLKDRIHRRPGWPVESRRVHLRRRSGDMLR
                     LARGIVRAYFDLQEVWLATRGRARWQENMDDLRRRYEELRERLGKSASEAQLALAARW
                     DQSRELLQRTGHAAGDRWRRRRQWLHRVNPLVVHATTRQHLNAYWRQTWDKFRRGRWH
                     RINPFKLGWNLLRDVKVCALFSLSFLQSLAR"
     gene            11554..12699
                     /locus_tag="PLS90_06640"
     CDS             11554..12699
                     /locus_tag="PLS90_06640"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK02118.1"
                     /translation="MSQLRVPLTDVTLGAEEAAAAARVIRSGWLTMGAEVEAFEREFA
                     DAIGAQYAVAVTNGTAALQLAYRAAGLGLGDHFAIPALTFVATMNAGLALGGRPILID
                     CTDENDLTLSPDDLECKITGRTRLIVTMAYGGFAPDMDRILGLAEQHGIAVVEDNAHA
                     PLARLDVQCLGTFGMAACYSFFGNKNMTTGEGGMIVTDNEAVAAELRLLRSHGMTHLT
                     WERHQGHAAGYDVLIDGYNARMDEVRAAIGREQLRKLPAANEARARAARKLRERLIAL
                     EINGLRIPFWNPRGEPVHHLFVILLPPRTVRDAFRAALMARGVQTSIHYPPLNSFTHA
                     RQLFGSTNDDLPVLQSIADRLVTLPLGPTLTEEQIDWVAESVRLALK"
     gene            complement(12753..13985)
                     /locus_tag="PLS90_06645"
     CDS             complement(12753..13985)
                     /locus_tag="PLS90_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02119.1"
                     /translation="MRRAASILALLLVIGLAACQRVTTRGPRYLDEASLANDPPYTLA
                     ILPTTDRTNHPELAQTVREVLYEAISTLPYADRELATVDRLLGQIASRRGVALAHLPP
                     EAMVSPELGEAVLFSELVDVRRLYLLFYAHNRFDLDLKLVDTRSRKVLYRNLFVFYDR
                     TGGPSITVLGLFERSISSLMHLRERALRESLEEAAQEIVTLMPRPTFDVARGELLRIS
                     RVTVAPRDKVLGAGDTVQIHTVATPGCTGRFSIGSVKRDLPLREAQPGHYYGQYEIVP
                     GDQSPFAVVEVQLALPATSETLEFAASGQPLVIDTEPPPPPRIARWWQTGAGVFFEIE
                     PGGIPRDDNDLPVAYNIYRRLAGQGEFQKTGEAREPVFRDGTARGGQRYEYYALAVDR
                     AGNISAPSGIVTITPRRH"
     gene            complement(14022..14738)
                     /locus_tag="PLS90_06650"
     CDS             complement(14022..14738)
                     /locus_tag="PLS90_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK02120.1"
                     /translation="MALETRHDLLHPPLTEPAAELPTHGATLLVNELYASIQGESTHA
                     GRPCGFIRLTGCPLRCVWCDSAFAFQGGERLEIAEILRRVADFGLPLVEVTGGEPLAQ
                     PNCLPLLRALCDAGHEVLLETGGSCDVAPVDPRVGIVLDIKCPGSGESHANHWENLTR
                     LRPRDELKFVLLDRADYEWARRLIHQYRLAGTRPIHFSPVHGGLAPAALAAWIIADRL
                     DVRLHLQLHKQIWPGRTHGV"
     gene            complement(14932..15306)
                     /locus_tag="PLS90_06655"
     CDS             complement(14932..15306)
                     /locus_tag="PLS90_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPK02121.1"
                     /translation="MQPEPMSAPTGPSVEERQWAMACHLSAFATFVLPMIGHLVGPII
                     VWVLRKDRMPYVDLHGKEAINFQITCTIYMVICIVTLFILIGVLLSIALGLFWLIFTI
                     IGGIKAYNNEPFRYPATIRFIK"
     gene            complement(15352..15747)
                     /locus_tag="PLS90_06660"
     CDS             complement(15352..15747)
                     /locus_tag="PLS90_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010871600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPK02122.1"
                     /translation="MDLVQPDVTVEPTGGPTRDERNWAMWVNLAGFAGYTCIPFASII
                     APLILWIMKKDQSAYINQRGKLALNFQITCVIYAIVGMVVQAFIFGLLGYLILVAVFV
                     YHLVFTIQGALKASGNEPYAPPGVLPLMK"
BASE COUNT         2598 a         5345 c         5291 g         2647 t
ORIGIN      
        1 cacggttcgg catgacccgt acttcccctc cacggccggg cgcctgatcg gctcgtcgag
       61 ccgctcgatc atccgccgcg ccacccgccg cgccgcctga tcctcgtgcc aggcccacag
      121 cgcggccagc cccgccagcc cctcgcgcag gttgtgcaga atgatcgtcc gcccgccttg
      181 ctcggtgggg aagccgttca ggccgctgac ttcgtcaaac gcggccaggt gccagcgccg
      241 ccacaactcg accacctcgc tctgggcctc cagcccgaag acctggtgcg cgaggtaaag
      301 cgcgtcgagc gcgcggccgg gcagatgcgg cacgtcgaac acgtgccggt gatagagatg
      361 cggccgcgcg gccagcagac tgacgccgaa gtacggcagg tcgtcccggt cccggtcgaa
      421 aatgcgctcg atgttgtgaa tcgccagccg tgcgacgcgg ctcaaagagg taaccgtggg
      481 catgtatcag gtcctcgcct cagtagatgg agggatccgg caaccgaggt tgcacgcttg
      541 cggccggggc cgccgcggcc agggcggcgg cggccggcgc cggaacatcg atccagggct
      601 cccctgcccc gaccgcccgc cgggggggca tccacgccgg cggagcgttt acaaatgaaa
      661 caacccgccc gggccgaaat acttcatgtc gccgtcgctg atcgttccgt tcgtcgggtc
      721 gtaggggatc gggcctttcc cttcgcgcgc gccgctgaaa cggtcgttat cgcggtcggg
      781 ccccaggccg cgcatctgat acaccgcgtt gatgcggacg ccccgctggt cgttgaactc
      841 gacgagcgag gccgccagca ggtccgtgat caggaaataa tcgaaggtct tatactcgct
      901 gggcggcacg gcgcccggtt cgccgaacag gttgaacaca tcgtccggga tgctcgtcac
      961 gtaagccacc ggcgtggtca gcggaatcag ccgccgcaga tagctgcgaa aaatgcccgg
     1021 ggcattgaca tcgatcagcc ccgtcgggta gtgggccgaa tccacatggt aggcttccag
     1081 gccgacagcg agcgagcgca tgtcggccat gttgcgcgag accttcgagc gggtctgggc
     1141 ctcaaggaaa ttggggacgg caatcgcggc gaggattgcg atgatcgcca ccacgatcag
     1201 cagttcaatc aaagtgaaag cgtcacgccg gtgtgtcacg ggatcatcct ccttggcaag
     1261 ctccaggggt ggaaggctgg cgcgaagcgc agcgcctcgc cgctatcatt aacgttaatg
     1321 cagaattgcc cgacgactcc ctgcctcagc ccccgcccgc gcccgcatct ggccgatcct
     1381 attgattcaa aaaaccccaa ttcctgccga ttttctatcg gaatcgtaga aaccgggggg
     1441 cgcatcggaa accgccgggc ggcgaggcgg gatctccttc atcaggggat gataacgtta
     1501 actaccacgc gccggcccac ctgtcaaccc caaatcgcac ccgcgcgccg gctgcggcct
     1561 cctcgcgccg gtccattgcg ggtcgcgggc aggcggccag ccccccctat ttcggcgttt
     1621 tcctcggcgc tgcggtcgac cgccgcacct tcagttccgg ttccagcagg atctgatggc
     1681 gtgcatcctg ccacagcttc tcgcccagca actgcagcat cagcttggag cccagctctc
     1741 ccagccggcc ggccttctgc gccaccgtcg tcagcggcgg gatgcagaac tcggccatct
     1801 cgatgtcgtc gtagccgatc accgacacgt cctccgggat ccgcaaaccg gtctcgttga
     1861 gccggtgcat cagccccagc gcgaccgcgt cgcagttgca catcacggcg cgcactcccc
     1921 ccttgaggat gtcccggacc agccgcgcgc tgtcttcata ccctgagcgg atcgtgttct
     1981 gttcggtgtc gggatagcgg ctgacctcca ggtcgacgcc gctggcggcg atttcatcga
     2041 cgaacccggc gtagcgcggc ggtttcaagt cgcgctccca gtggccggtg tagtacgcga
     2101 tccgccggtg tcccagctcg accaggtggc gcgccgccag gcgccccccg gcatagtcgt
     2161 cgtagccgac gaaatgcggc ggcatcgcgc cccccgacat gatcagcgac acatggggca
     2221 ggccgtggcg atcgaccatc tcggcggctt tatcggtgat ccgcgtgatc gagatcagcc
     2281 cctcgatccg gtgctccatc agcagctcca tcccctcgtg cagccggtcc ggatcgttgt
     2341 gcgtattgac gatgtaagcg taataatgcg aactctggag acaatcctgg atcgcgatcg
     2401 tggtctcggc gaagaacggc acgttcaggt cgggcaccag cacgccgatc gagtgcgagc
     2461 gcttcagcac cagcagccgc gccgccgtat tgggccggta gcccagccgc cgtgcgatcg
     2521 actgcacgcg ccgccgcgtc tcatgcttta cccccggcat gccgcgcagg gcgcgcgata
     2581 cggtattgat cgagacattc gcctcgagcg cgatatcctt caagctgcag ggcatacggc
     2641 ctccatctca gacaattcat ctctcgtccc tcgtcctcgt gctcggcccc ccacggccag
     2701 tctgagaact cggctctcga aaacccgcgc gcttcgcccc tcgcgacctc tcccggatct
     2761 cctcaacgtt aaggttcatt atggcgacct gaaatggttt gggcaagatt ttggcgtcat
     2821 cgcccgcagc gttgcgttcc ccacccaccc gtcggtccgc accgccgatt ccgctttgat
     2881 tttctgtact ccggcgctat tctgctggtg cgttcatacc acgtggcgtt gggctgctcc
     2941 gcccccgttc gcgctgctga tcctgctgct cccgttgccg ctgacgcatg agaacgctca
     3001 atacccccct gctgctcttc atgttgaccc tggcgctggt cacctgcggc atcgtcgccg
     3061 tctacagcgc cagcgcctcc tccgcctatc tgaccaaaca actcctgttc gccggcatcg
     3121 gcctggcggc gatgctcttc tgctatgcga tcgattacca tgtcctcaag cgcgccagct
     3181 tctggctgat gctggtggcc ttcggtctgt gctgcctggt gctggttccg gggatcggca
     3241 acgaagtgaa gggggcgcgg cgctggctgg cgctggggcc gatctcgatc cagccctcgg
     3301 agctggcgaa actggcgctg atcatctaca tggccaagat gctctcggag cggcgcgccg
     3361 tcatcaagtc gttcttctcg ggagtgttcc cggcgctggt cgtcaccggc gcgttcgccg
     3421 cgatcatcgt catcgagccc gacttcggcg ccgccttcgt cctctgcctg atcacgttcg
     3481 gcatgtggct ggccgccgag atgcgctggt ttcatctggt cggcctggtg ctggccgccg
     3541 tcccggccgc gctggtggcg ttcctctcgg agccctaccg cgccaaacgc ctgatcgcct
     3601 tcatgttccg cgacaaggag acgctgctcg gcgccggctg gcaattgcac cagtcgctca
     3661 tcgcgatcgg ctcgggcggc ctgtgggggc tcggcctggg cgagagccgg cagaaatttc
     3721 attacctcag cgaggcgcac accgacttca tcttcgccat catggccgag gaactgggct
     3781 tcgtcgccat gtcgtgcatc gtggggatgt tcctgctgat cacggtgctc ggctggaagg
     3841 tcgcctggcg cacgaccgat ctgttcggca gcctgctggc caccggcgtt acgctgatgg
     3901 tgttcatcgg cgcctcgatc aacatgtgcg tcgtgctggg gctgctcccc accaaggggc
     3961 tggtgctgcc gttcatcagc gccggcggtt cctcgctgat cgtctgcatg gcggcgatgg
     4021 gcattctgat gaacgtggcc gccagtctgt atcaccacca gggcgcgcgg gcggtcacgg
     4081 catgagccgg ccggcatccc gctctcgcgc cgcaacgcgc gcgctcaaca cggagatcct
     4141 cacgccacga tgctcagacg aacccgttcg cccatccgca cgattgttct gcctcatggt
     4201 ttccgggcgg ggacgcccgt gccggcgcgc gtcggccccg cctggtccgc ggccgcgcgc
     4261 ggacgatcca ggccccttgc cgcccgcggc gcgcggcccg atggcgcggc catgaccgat
     4321 gccgccggac cgcgtctgct gctggccgcc ggcgggacgg gcggccacat catgccggcc
     4381 gtggcgacgg ccgaggcctt gcgcgagctg gcccccgacg tccagattga ttttgtctgc
     4441 ggccaccggc cctcggagtg gcagatctat cgccgcctgg ggatcgaacc ctggacgctg
     4501 ccgctgacgt tcaatcgccc cggcttgatg aaccgcatgc ggtttctccg ggagttcctc
     4561 gccgcgctgc gcgaagcgcg ccagcgcctg cgcgcgcacc cctgcgatct ggttgtcggc
     4621 ttcggcagct acatgtcggt gccgcccgtc ctcgcggcgc ggctggccgg catcccggcg
     4681 gtcgtcgtcg agcaggatct gaggcccggc atggctaatc gcctgctggc gccgctggcc
     4741 cgcaaagtcg ccgccgcctt cgaaacctcc gccggcttcc cgcgcggcaa gacgaccgtc
     4801 gtcggcaatc cgatccgcgc cgacatcatc cggccggtcg accgggtcga ggcgcgccgc
     4861 ttcttccgcc tcagcccgca tcggctggtc tgcttctgcg tcggcggctc gcagggggcg
     4921 cgcgggatca atcagttgct gctggacctg ttgcggcgcc agcacgccgg cggcgaggaa
     4981 accgctggcg cggcggcgca atggcagtta ctctggtcga cggggccggc tcacttcgat
     5041 catgtcgtgc gcgccctgcg ggagatgggt gtcgaccccg ccgaacacag catcaacccg
     5101 tatatcgaca agatggccct ggcctacgcc gccgcagact tggtcctgtg ccggaccggc
     5161 gcgctgaccc tggccgaatt gaccgccgtc ggccggccgg ccatcctcgt gccgctgcca
     5221 tcggcggccg gtggacatca ggccgccaac gcgcggcgcc tcgtcgaggc cggcgccgcc
     5281 gaactgattc tggagaccga ccccgcggcg gccgagcggc tcgaaacact cctcgcgcgc
     5341 tgggtcgagc agccgggttg gctcgccgag atggcccacg ccgcgcgcaa gctgggccgg
     5401 ccgcacgccg cgcgcgatct ggcccggatg ctgctcgatt gcctcccttc ccgcggggat
     5461 tgacccgccg gcccgcgccc cccacggcgc ccaatcccct gcgcacggag caccccatgg
     5521 acgacctcag gcagttccaa cgcatccatt tcatcggcat cggcggggtc ggcatgagcg
     5581 ccatcgccac ggtgctgctc caccacggtt tcactgtttc aggcagcgac cgcgccaaaa
     5641 gcgccgccac gctgcgcctg caggccgccg gcgcccgcat tttccacggt cacgaagcag
     5701 cgaacatcga cgacgccgat ctggtcgtcg tctcgacggc gatccgcccc gacaatcccg
     5761 aactggtcgc cgcccgcgag caggagcgga cgatctggca ccggtcgcag gccctcgcct
     5821 gcatcatgcg catgggccgc tcgatcgcca tcagcggcac ccacggcaag accaccacca
     5881 cggcgctgct cggcctgatg ctgaccgagg caggctgcga tccgaccgtc gtcatcggcg
     5941 gctcgctccc caacttcggc ggggccaacg cccgctgcgg caacggcccg tgggtcgtcg
     6001 tcgaggccga cgaatccgac gcctccttcc tgcgcatgga tcccgaccgg atcctgatct
     6061 ccaacatcga ggccgatcac ctggattttt actccgatca ggccgccgtc gaggcggcct
     6121 tcaagaaatt tctgggccgc ctgcgcgacg gcgggcgcat gatcctctgc atcgacgacc
     6181 cggccctggc cgccctggcc cgcatcgacg agcaccacgc aatcacctac gcgctggccg
     6241 accccgccgc cgaaatgcac gccgcggcgg tcgaaacgat cgacggcggc ctcggctccc
     6301 gcttcacccc cgtctggcgc ggccaggcgc tggaccccgt cgagctccac gtgccgggtc
     6361 gtcacaatgt ccagaatgcc ctcggggcgc tcgcctgcgc gctggagatc ggcgccgact
     6421 atgaagggct gcgccgcggt ctggccgaat tcgtcggcgc cggccgccgt ttccagttca
     6481 agggcgtgtg cggcgacatc gcggtctacg acgattacgc gcatcacccg accgagatcg
     6541 ccgcgacgct ggccgccgcc cgcgcacaga tcgagtcggg ccgcgccgcc cgcctcgtcg
     6601 ccgtctttca gccccaccgt ttcacgcgca cgcagcacct ggccgaccag ttcggcgatg
     6661 ccctcgacgt ggccgatgtc gcggtggtga cggaagtcta cagcgccggc gaaaacccga
     6721 tcgccggcgt gtcgggccgg ctgattcacg accgcctgct ggcgctcggc caccgctccg
     6781 cccaatatct gccgacgctg gacgaagtgc gcgcggggct ggctgagatc gtcatgccgg
     6841 gcgatctggt gctgaccctc ggcgccggca acatctggca ggtcggcgag ggcctgctgg
     6901 ccgagctgcg cgagcggagc gccgagctgg cctgacaatt tccacatctc gtttcatccg
     6961 acccggggac cgacgccatg gcccgcgccg ccgcccgttt gacccgcgcc atcgccctga
     7021 tcctggcctt cctgccggga ctggtcggct gcggccgcga gccggtcgag accgccatcg
     7081 cccggctgga ggaacgcccc gcgatttacg ccgaccacga ggtccgcgtt cgcggcaccg
     7141 tgacggctca cctcggcacg aagaaccccg gcgaaccgat gcggtatgtc ttgcgcgacc
     7201 ggctctccga gcgcgagatc ctggtcgttc cggccctcga acgcaaggac gagctccccg
     7261 gcctgggcgc cgaggtcgcc gtcgtcggca gggtcgcgcc ggccgcgcag gacgccggcg
     7321 gcgccgcgag cggcgcggcg ctggtgttgc gcgagaaatc cgccggcggc aacctcaccg
     7381 acgagccgct ggtcttctgg ggggcgatgg ccgtcggcct cgcgctcctc ggcgcggtgg
     7441 gctggctgtt gctgagcatg atgcattccc cgcgccacgg cgcgcgttat gcgcgcaccg
     7501 gtcacgtcaa cggcctcggc cgccgcacgc tcgaaacggc cgcgcccgcg ccggccgccc
     7561 cgcggccccc cgccggcgag atatcgagaa aaccagccga tgcccccgag gacgactcga
     7621 gcgtgggcga catgatctga gccgctccgc gcattcgctc cgacggcagc tgtgggctct
     7681 catcgtaatc gccggcgaag gtctacccga aactcggctc tcggctctcg tcctcggctc
     7741 tcgaaaaccc gccatttgat tccgcgttga tagttaatgc ctctcggcgg gcactgactc
     7801 aatcccgatc ctgaatgcgc cctcgcagtc tttgcgggtt gcacgcgcca tcgcggcaaa
     7861 tcgcttggcc gatccggcgc atgggtggtt aaatagactg tcatggcgca gatccatccc
     7921 accgccgttg tcgatcccag cgcccggctg gccgacgatg tgacggtggg gccgtatacg
     7981 atcatcgagg cggatgtcac ggtgggggcg ggctcgcgga tcggcgcaca cgtgacgctg
     8041 ggggaacggc tccggctggg atgccgcgtg cgggtattca actacgcctg cctgggcacg
     8101 gccagccagg acctgaagca tcgcggcgag atttcgcggg ccgagatcgg cgacgactgc
     8161 atcatccggg aatttgtgac cgtcaaccgc ggaacgcgcg agggaagcgt aacgcgcgtc
     8221 ggccgcggcg tcgcgctgtt ggcctatagc cacgtcgcgc atgaatgcgt gatcgaggac
     8281 ggcgcgatcc tggtcaacgg ggcgacgctg ggcggcgagg tccacatcgg ccggcgggcg
     8341 acgctgggcg gcctggtggg cattcaccag tactgccggg tcggagatta cgcgttcgtc
     8401 ggcgccaaca gcaaggtgac gcaggacgtg ccgccgtttc tgctggtcga cggtcatccg
     8461 gcgcggccgt tcggaccgaa cgtggtcggc ctgcaacgca acgggttcag cgaggaacag
     8521 atccgggaga ttcatcggct gtatcagatc gtgttcgacc gcagccgcgg cctgaacgat
     8581 aacctggcgt acattcggga gaaacatggg gcatcgccca tcgcgcggac gatcctcgaa
     8641 ttttgccgcg acggcagccg cgggatcgtg cgaccgcgcc agcggcgacg catggcgatg
     8701 atccccggcg acctgcaatt cacctgattc atttggcctc gagcgtctcg gatttctggc
     8761 tcaccgggct tgtggaaaac ctgtggaaaa gtgggttttg aggccgattg acaagggttt
     8821 tccacgggtc cgctgggcgc cggaacacgg tcatccggga tgtgcgatat cccacaaaaa
     8881 ttttcttgaa taaacacgga caactgattt tgcgagagtt atggaaaatg taaggttttc
     8941 cgcgcggaac cgctcatgcc gggcccgtcc gattggggct ggcctcatcc gaatcaaaaa
     9001 ccgacctgtg aataaaacga acgccgaaac gcggcgcggc gcttgatttt gtgagattta
     9061 caagtcggga cggaggcgct ttccgagggc ttgccccccc gattgatcag ttcgataaca
     9121 aattctcgcg aaaacgagga cgaggacgac ggacgaacaa tccgcgtcac cgcgccaggc
     9181 tctggagaaa cgaaagactg aacagggcgc aaaccttcac gtcgcgcagc agattccagc
     9241 ccagcttgaa cggattgatc cggtgccagc ggccgcgccg gaacttgtcc cacgtctgcc
     9301 gccaataggc gttgaggtgc tggcgggtcg tggcgtgcac gacgagcgga ttgacccggt
     9361 gcagccactg ccggcggcgg cgccagcggt cgcccgcggc atggccggtg cgctggagga
     9421 gctcgcgcga ctgatcccag cgggcggcga gagcaagctg cgcctcgctg gccgatttcc
     9481 ccagccgctc gcgcagctct tcgtagcgcc ggcgcaggtc gtccatgttc tcctgccagc
     9541 gggcgcggcc gcgggtcgcg agccagactt cctgcaaatc gaaataggcc cggacgatgc
     9601 cgcgcgccag ccgcagcatg tcgcccgaac gccggcgcag gtgaacgcgg cgcgactcga
     9661 ccggccagcc cggccggcga tggatgcggt ccttcagccg cacgaagccg cagatcatcg
     9721 gatgggtttc ctcgacgaac gcggcgtagc gataccacat gaaatttttg aacaggcccc
     9781 agtaagtacg ttggttggcg cgcgcgagga tctgcttcat gttgtcgagc gaatagaact
     9841 cgcgccaggc ctcgcgatag gcctcaaacc actcctcgcg gctcatccgg gggtgttcgg
     9901 tgaccggatg gaacgagtcg aaccggttgt agtcgggatc catccattcg ccgcggcgcg
     9961 ccatgttgac gtgatcggcc gagccgggca gcggcgtcag catgaagaac gaagcctgat
    10021 cggcccccag ttccttgagc cggcggacat cggcgcgcac ggattcaacg gtgtcgtgcg
    10081 ggaagccgat gatgtagccg acatggcagg cgattccgaa cgcgtgccag gcgtcgaaca
    10141 tctcctggta atcgaccgcg aggttctgct tcttgccggc ggcgtccagg ttgtcggtgt
    10201 tgatgctttc catgccgatg aaaacctggc tgcagccggc ctcggccgct ttgctcatga
    10261 agcgccggat gcggtaggca aggacgtcga cctgcatcat gaactgaaac tggagccctt
    10321 cctcgcggcg cagggcgatc agcgcgtcga agatcgcctc ccagtgcgga ttgcgcgaga
    10381 agttgtcgtc ggtgaagaaa taatagtcga tgtcgtgggc ggcgttggcg cgcacgcgct
    10441 ccttgatcgc ctcgggattg cggcagcgca tctggcgccc ctggacgttg atgatggtgc
    10501 agaacgaaca gccgaacggg caaccgcgcc ccgcgtcgat cgtccccatg aaagggtagg
    10561 cgtagcgctt catcagccgg gggtcgacga ccgggatggc ggcggaccag atgtcggggc
    10621 ggtcggcgat gtcgtagaac ggccggagcc ggtgatgcag cgcatcgcgc aggaggtcgc
    10681 cccagcagtt ctcgacctcg cccttgacca gcgtcacgcc gtcgtcgagg agggcctggc
    10741 actcgggggg gagcggcccg ccggcagcgt agtccgacag ggcgatggcg cccgagacgt
    10801 ggaacccgcc gatcatcacc tgcatgccgg cggcgcggaa ctcgcgggcc agatcggccg
    10861 cgcgcgggaa ctggttggtc tggacgccgc acagcgcaac gacggcgcgg gtatcggggc
    10921 ggcgcgcccg gcgcgccagc cggcggacat cgaccggctg aacgtactcg tccagcatca
    10981 gggtctccac cttgaggcgc cccagcgcgc cggtcgccgc cgcctcgcgg gtcagcccgg
    11041 ccagcgcagc caaggtgttg ctcggcagca cgccgcggaa aaatcgcatg atgtagccgt
    11101 cgtcgtcgta tttgctgggg cgaatgagga tcaccaacag gcgcgtgacg ccggcgggcg
    11161 ccgcctcaat ctttggacgc gggtcggcgg cagacgcggt cgttccggaa gcgatcataa
    11221 gggggggccg tttcgagttt cgagggttcg ttgaaggatc agcacgatgg ggcgggatgg
    11281 acgattggcg aagcggttcg gctcgcgatc acggtgagtc accatagcgc aacccgcccg
    11341 gcaaggaaag gagtttcaca tgcccaagtg gggataggtt gggtcggcgg gatcccgggt
    11401 gctctaccgt attccggccc cgcgcccctc ccttctgtcc cttgtgtccg ttgtatgact
    11461 tacctcccct ggccggaatg gcccagccgg cgtggcttga acgatcccgt cacttggcgg
    11521 acaatggcct cagatccagc gaacgggggg agcatgagcc agttacgcgt gccgctgacc
    11581 gacgtgacgc tgggggcgga ggaagccgcc gccgccgcac gggtcatccg ctccggttgg
    11641 ctgacgatgg gcgcggaggt ggaggccttc gagcgcgaat tcgccgacgc gatcggcgcc
    11701 cagtatgccg tcgccgtgac caacgggacg gccgcgctgc aactggccta ccgcgcggcg
    11761 gggctcggcc tgggcgatca cttcgccatc ccggccctca ccttcgtcgc gaccatgaac
    11821 gccgggctgg cgctcggcgg gcgaccgatc ctgatcgact gcaccgatga gaatgatttg
    11881 acgctctcgc cggacgatct ggaatgcaag atcaccggcc gtacgcggct gatcgtgacg
    11941 atggcctacg gcggattcgc ccccgacatg gaccgcattc tggggctggc cgagcagcat
    12001 gggatcgcgg tcgtggagga caacgcgcac gcgccgctgg cccggctcga cgtccagtgc
    12061 ctcggaacgt tcggcatggc cgcctgctac tcgtttttcg gcaacaagaa catgacgacg
    12121 ggcgaaggcg ggatgatcgt gaccgacaat gaggcggtcg ccgccgaact gcgcctgttg
    12181 cgctcgcacg ggatgactca cctgacttgg gaacgccacc agggtcacgc cgccggctac
    12241 gacgtgctga tcgacggcta caacgcgcgg atggacgaag tgcgagcggc gatcgggcgg
    12301 gagcaactgc gcaaactgcc ggcggcgaac gaggcgcgcg cgcgcgcggc gcgcaagctg
    12361 cgcgaacggc tgatcgcgct cgagatcaac gggctgcgca tcccgttctg gaacccgcgc
    12421 ggcgaaccgg tgcatcacct gttcgtgatc ctgctgccgc cgcggacggt gcgcgatgcg
    12481 tttcgcgccg cgctgatggc ccgcggcgtg cagacgtcga tccactatcc gccgctcaac
    12541 agcttcaccc acgcgcggca attgttcggc tcgaccaacg acgatctgcc ggtgctgcaa
    12601 tcgatcgccg accggctcgt gacgctgccg ctgggaccaa ccctgaccga ggaacagatc
    12661 gactgggtgg ccgagtcggt gcggctggcg ctgaagtgag gcgcctgccg acggcgtggc
    12721 ccgatcggcc cgtttgattc gagacacttg tgtcagtggc gccgcggcgt gatcgtcaca
    12781 atgccgctgg gggcggagat attgcccgcc cgatcgacgg cgagcgcata gtattcatag
    12841 cgctggccgc cgcgggcggt cccgtcgcgg aagaccggct cgcgcgcctc gccggtcttc
    12901 tgaaactccc cctggccggc cagacggcgg tagatgttgt atgcgacggg cagatcattg
    12961 tcgtcgcggg gaatgccccc cggttcgatt tcaaagaaaa cgccggcgcc ggtctgccac
    13021 cagcgggcga tgcggggggg cggcggcggc tcggtgtcga tcaccagcgg ctggccggag
    13081 gcggcgaatt ccagcgtctc gctggtcgcc ggcaaggcga gctgcacctc gacgacggcg
    13141 aacggcgatt gatcccccgg cacaatctcg tactggccgt agtaatgacc cggttgggcc
    13201 tcgcgcagcg gcagatcgcg tttgaccgag ccgatcgaga accggcccgt gcagcccggc
    13261 gtggccacgg tatggatctg gaccgtatcg ccggccccca ggaccttgtc gcgcggcgcg
    13321 acggtgacgc ggctgatccg cagcagttca ccgcgggcga cgtcgaacgt cggccggggc
    13381 atcagcgtca cgatctcctg ggcggcctcc tccagcgact cgcgcagggc gcgttcgcgc
    13441 agatgcatca gcgaggagat cgaccgctcg aacaggccga ggacggtgat cgatggaccg
    13501 ccggtgcggt cataaaaaac aaacagattg cgatagagaa ccttgcggct gcgcgtatcg
    13561 accagcttga ggtcgaggtc gaagcgattg tgggcataga acaggaggta cagccgacgg
    13621 acgtcgacca attcgctgaa cagcacggcc tcgcccagtt caggcgagac catcgcctcg
    13681 ggcggcagat gggcgagcgc gacgccgcgc cggctggcaa tctgccccag cagccgatcg
    13741 accgtggcca gctcgcggtc ggcatagggc agcgtggaaa tcgcttcata gaggacttcg
    13801 cggacggtct gggccagctc gggatggttc gtccgatcgg tggtcggcag aatcgccagc
    13861 gtgtagggcg gatcattcgc cagcgaggct tcatcgaggt agcgcggtcc gcgcgtcgta
    13921 acgcgctgac aggccgcgag gccgatcacg agcaacagag cgaggatgga cgcggcgcgg
    13981 cgcatgatcg cttccgaacg catacgagga gtgacggcgg atcagacgcc gtgggtgcga
    14041 ccgggccaga tctgcttatg aagctgcaag tggaggcgaa cgtcaagccg atccgcgatg
    14101 atccaggcgg ccagcgcggc cggggccagg ccgccgtgga cgggcgagaa atggatcggc
    14161 cgcgttcccg ccagccggta ctggtggatc aggcggcgcg cccactcgta gtcggcgcgg
    14221 tcgagcagaa cgaacttcag ctcgtcgcgc ggccgcagcc gggtcagatt ttcccaatgg
    14281 ttggcgtggc tctcgccgct gccggggcac ttgatgtcca ggacgatgcc gacgcgcgga
    14341 tcgaccggcg cgacgtcgca cgagccgccg gtctcgagca ggacctcgtg gccggcgtcg
    14401 cacagcgcgc gcagcagggg caggcagttg ggctgcgcca ggggctcgcc gccggtgact
    14461 tcgacgagcg gcaggccgaa gtcggccacg cgccggagaa tctcggcaat ctcgagccgc
    14521 tcgccccctt ggaaagcaaa ggcgctgtcg caccagacgc agcgcagtgg gcagccggtc
    14581 agccgaatga agccgcacgg gcggccggca tgagtcgatt cgccctggat cgaggcgtaa
    14641 agctcgttaa cgagcagcgt tgcgccgtgc gtgggcaatt cggccgcggg ctccgtcagc
    14701 ggaggatgca gaagatcgtg gcgtgtctca agggccatcg tcgctcccgg tccggtgggg
    14761 caggggaccg gtcgccattg tagcagttcc cggcggcgag gtcagacttt cttcgcggcc
    14821 aggcccatcc gctgggcgac ccgcagctga acgcaagctt atcggtatcg gtatcgggat
    14881 cgcgatcgat tcaatacccg ctcgcatccc gcggggaagc cggacggccc cctacttgat
    14941 gaagcggatc gtcgccgggt agcggaaggg ttcgttattg taggccttga tcccgccgat
    15001 gatcgtgaaa atcagccaga acaggcccag ggcgatcgac agcagcacgc cgatcaggat
    15061 gaacagcgtc acgatgcaga tcaccatgta aatcgtgcag gtgatttgaa agttgatcgc
    15121 ttccttgccg tgcaggtcca cgtagggcat ccggtctttc cggagaaccc agacaatgat
    15181 cggcccgacc aggtgaccga tcatcggcag cacaaacgtc gcaaacgcgc tcaggtgaca
    15241 cgccatggcc cattggcgtt cctccacgct cggcccggtg ggagcgctca tcggttcggg
    15301 ttgcatctcg tccacggttc acgctccggc gattcgaaaa gggttgcacc gtcatttcat
    15361 caggggcagc acgccgggcg gtgcgtaggg ctcgttgccg ctggccttga gggcgccctg
    15421 gatcgtgaag acgaggtgat agacaaacac ggcgacgaga atcaggtagc ccaacaggcc
    15481 gaagataaac gcctgcacca ccatgccgac gatggcgtag atgacgcagg tgatctgaaa
    15541 attgagcgcc agcttgcccc gctgattgat ataggccgac tgatctttct tcatgatcca
    15601 gaggatcagc ggagcgatga tgctcgcgaa cgggatacag gtatagccgg cgaagcctgc
    15661 caggttcacc cacatcgccc agttgcgctc gtcgcgggtt gggccgcccg tcggttccac
    15721 cgtcacgtcg ggctgaacta gatccatggc ttctcgctcc atgcagggat gccgccgggg
    15781 agccgtaggg ctccgggatc gcaaacgtcc gccgtccgat ggcgtaagtt tgcggggaac
    15841 aggaggcagg tgcaatcaga atcggcgtcc ccggccgcga a
//
LOCUS       DAOPCO010000065        15747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3799_length_15747_cov_5.196597, whole genome shotgun sequence.
ACCESSION   DAOPCO010000065 DAOPCO010000000
VERSION     DAOPCO010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3799_length_15747_cov_5.196597"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..2468
                     /locus_tag="PLS90_06665"
     CDS             78..2468
                     /locus_tag="PLS90_06665"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPK02123.1"
                     /translation="MSAASAPPIRNSSGERQRNSEWGARWRLTVFTTRLGELGRANFY
                     KRALLKILHGGQFWSDEWHFDWRRAGLIVRSADAGDQAMDFHHVDSGPRIHAEHLRDA
                     LLNAWSTTEDSLSPTDRFLGEGCDYTVGLELLPELIEQANAAFDDRQFIHGNLPDYIQ
                     RLRETISMDELRVVEGELRDPPITAVQGNALATRLYLKRLNRLAEHALIQGAEPLVAM
                     ARMMGLPCPAGFLENAWRYLLLSHSHDAINGVTLDKISRDVVHRLEQVLEIAHALTIH
                     AMTGMVGRMNLSGFDAGDVLLVVFNPLPMARREVLFAALDTPREQDVARFSLVDTDGR
                     RRPVQWIDRHEHLVPVHDACARPRPFYCDRHTVYFDTGEVPAGGYKVYRLVAESRQDR
                     SLDFLYERIDQGSQLCDGGMGMENEFLKVTFQSDGTFDLLDKTTNRAFRGLNAYEDGG
                     ELGDYWHRTVPNHDEVISSRGFPHRIRLVRDGPLVTTFAIETTMRVPARADWSKRARG
                     ADCIDLRIETEVSLAAGQRRVDVTVRFNNQAEDHRLRALIPTWIQAEHSDAEGHFLVL
                     RRPITLARDVNGVGPGGMKTLPQHTFVDLSDGEGGLAILNRGLTEFEVSEDASRTIAL
                     TLLRTAPMKICTEFRTPTVDPNQKGGQCLGDHVCEYSLYPHTGDWSQGGVNREAQLYT
                     VPLRSMQTAAHARGHHAPTQSFYELKSDRVVAAAMKAPDDAQSGWDLVLRIYNPSPEA
                     DRAEWTFHQPLARAALLQLNEEEIAPLEVTDQRKVGLEVGPGKIVTVGIAFRESEP"
     gene            complement(2559..3311)
                     /locus_tag="PLS90_06670"
     CDS             complement(2559..3311)
                     /locus_tag="PLS90_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPK02124.1"
                     /translation="MMRSAQASAREPAAKPDASPNQEAAAYEQIFQWLEDYIDRNEVQ
                     LGDPLPSEDEIMRETQMSRSSVREAITRLRALGIVDTRRKRGMWLVRSPRLLDLIRLL
                     SRPNIPPNFMGHAFGYRCALELGLCSEIFRRATPQDIAELRAIFEKMVEHGHQPKVWN
                     DYDRQFHLKLIAITGNEIAIWMSQLLNPFFQTLSQYTTSLSDHVRALHESIVTSLEQR
                     NAEAFYQAMHEHNYWKLAFDKLEWTSFTKSTP"
     gene            complement(3572..6037)
                     /locus_tag="PLS90_06675"
     CDS             complement(3572..6037)
                     /locus_tag="PLS90_06675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK02125.1"
                     /translation="MESNQRIDWYRVKVAPDLLRELSAPNDLQGFLQAGGHVALAFVT
                     GLTAVLVYLNLSPWWLIPALLLHGTVVSTFGAASHELSHERVFRTGWLNRLFLNVVCF
                     LTWDNPRLYMLSHNQHHKFTLHQPDDLEVVLPQKATLLQLILVNFLDLRVLGKRIVDN
                     VGLALGRPRGTWYTQLIAGADAVTRRRVFNWSRLALLGHAAVLVAGALSGLWILPVVV
                     SLGRAYGGGLIMLMGLPQHIGLVDEVNDFRLSCRTYYLNPFLRFLYWRMNYHIEHHMY
                     PAVPCYHLPRLHEAIKHELPHCPNGLIETWVQIADILIKQKYDPSYQFRARLPGEPET
                     APVQATQGGASDAGLAEVARVWECQLCGFIYDEHEGLPEEGFPPGTRWEDIPEDWVCP
                     VCGVRKSQFKMVEIRREAAAAKPRIEIDSLADPIVIVGSGLAGYAVARELRALNSDAP
                     IQIVTRDGGESYYKPSLSEAIAQRKNPDDLVQSSSEAMSRRLRADVLTRTTVSAIDVA
                     ARRLRIPGREIPYSKLVLALGAEPIRPRLAGPLAGEVLCINQLDDYRRFRERLAPQAR
                     VVILGAGLVGCEFANDLALGGYAPCVLDLADAPLARFLPPQLGQALKTALESIGVQWH
                     FGEGLVSIEPGGDRPFRCTTHGNKTLEADLVLSAIGLRPNTELARAAGLAVNQGIQVD
                     SLLRTSAEDVYAIGDCMEFDGAVRPFVLPIQHAAPSLARTLLGAPSPVDFSRVMPIVV
                     KTSRCPVVIALPPGGAEGAWEIGGSDEDLEALLRDGAGRLRGFALLGRAVDKTDEYQA
                     LLDRQASAFAPLAEAGAAGSGRG"
     gene            complement(6000..7079)
                     /gene="pdxA"
                     /locus_tag="PLS90_06680"
     CDS             complement(6000..7079)
                     /gene="pdxA"
                     /locus_tag="PLS90_06680"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPK02126.1"
                     /translation="MTNPVKPLVAITQGDPAGIGPELCVKLTHEPSVLGFCRPLILGD
                     RVVLEDAARRLGRPLSATALTLAEFERTARVDLPDGPLVVDCACLTAPVAPGTPTRDG
                     GRASYAYIENAIRLALARKLDAVTTAPITKQTLKMAGIDDPGHTEIFGRLTGSRDFAM
                     MLYSPRLAVSFVTCHQSLRSVPDALRTEEIERLIHLTAETLRRIRGAEPRLAVLGLNP
                     HAGEGGMFGDEDERIVRPAVEHARAEGWNVEGPIPPDAAFMPRALQRFDGHVTLYHDQ
                     GSIPFKMISLHDGVNITMGLPIIRTSVDHGTAYDIAGQGKADPSSLISAIELAARLAA
                     PADADRPSSEDNRHGIESAHRLVSS"
     gene            complement(7132..8139)
                     /locus_tag="PLS90_06685"
     CDS             complement(7132..8139)
                     /locus_tag="PLS90_06685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK02127.1"
                     /translation="MKTRTGVREGVWPVLLTPFGDDLSIDWAALDEMIDYYIAEGVAG
                     VFANCTSSEIFDLQPEEMLQIARRITERAAGRIGVVATGNIGDTLEQQRESMRRLAAT
                     ADLDAVVISTSMLPAARPIHDEIRLLMDGGEMPLGIYEAPIPVHRTLQPGEVAELAAT
                     GRFVFLKETSRQVPVFSQKVELARDTPMQVFQANLACLLEGGEQTGAGFAGTLVNVAP
                     RLSRDYLLEPQRTTPWHHQARELMLSIGNIMNQTKYPASAKYMLQRLGLRIQTACRWH
                     VANGFSAGDIAVIDRFFDVARPAIVSVDGLWRGFRWHENDELIMKLREWSQLTTTPAV
                     D"
     gene            complement(8136..8969)
                     /locus_tag="PLS90_06690"
     CDS             complement(8136..8969)
                     /locus_tag="PLS90_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK02128.1"
                     /translation="MTIVNHNGRLDERQRYERDGFIVVQPILTELELRQARQAMEDVM
                     AGRYETGLAPRDAFRKPDDDPRLSLCKIDQPQVANRTIHRIISKPEIGEYIAALTGAE
                     LVQAWAVQLLYKPAGSEAASVGWHQDMAYWQDHWTPDSEVLTAWLAISDVAEESGPLC
                     FVRGSHRWGLRPGGSFFEKPDSQSRQSIPLPDGATWEEVTAPMPAGAFSVHHKYTVHG
                     SYPNRSNGPRMSFAIHLRTNRATPIHGRARVYDYVGKLSDESVCPILYRAGQTAEGVL
                     R"
     gene            complement(9046..11454)
                     /locus_tag="PLS90_06695"
     CDS             complement(9046..11454)
                     /locus_tag="PLS90_06695"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK02129.1"
                     /translation="MMPWRGAWRMFGPIASQKDPGKWRPWFEPRLEPTALDGSPEFLE
                     QADVDGVQEQGRQLIYDGQELDLQRMWHTNAGQGLLYLYRQEQSDGDALLPVEARARG
                     WLKVWVNGRLAYAGGNDGETWGLHRFEVPLQSGQNTLGFRLAAGRDGWGLALQRGVKM
                     LPPPETIGREVTGPGGAGADAWRVDGMLQAQDSAERLLLSAPAFQAATLRVAAPETGD
                     RLVKMRFKSLGALGRPVECFIALKDGVEVGYSGHLNAETGQYQVIWQVRENGRSVWEI
                     DHRPVTDWAGVSPLADFTEAQAALEQSRMDLDGQAGRAKDRAEQEWIAASRGDEITLR
                     VNGMENLVLRHRIAGSAGRGGELGLNLRGCSVELTEFNAQALPADIASRWALGDDPPV
                     RWDVAHPDPYRTDGGYPVMPGLEPITLYRANPEGWTFCHHAKIAAFGGRLYGSWSNSY
                     MHEDSAGTRVRGSISLDGGKTWSPSFVMLPSLSDPADDLEDPHKPGVSIWPNEFGGFL
                     AVEDRVYVLGRAGYRGGYLGQSGQMGFLARQILGVDEMGPVFWLNEHAPRGGEQYPGL
                     ANVTHEQLRADGQALLQQLRLPDDFMDRSVEPRDIYRGARPEAVDFSIISHDPIYVSP
                     DGMQVRVWRNYNGTHRIYVSTRHSDTEAWSLAVPTNIPDSPSGVTVGELPDGTVYLIG
                     NQIAGELDDPDARPYRNPTKVWPFAERDFQSEHFPRDPLVLALSRDGRGFDRAWAIRH
                     DTPNARFNGLGWIPGFQYPDAVIANGSLWIIYSVNKEDVEVLEIPLEKLAEAGESPAA
                     NAPD"
     gene            complement(11730..13190)
                     /locus_tag="PLS90_06700"
     CDS             complement(11730..13190)
                     /locus_tag="PLS90_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02130.1"
                     /translation="MHKGKHSLRQGDKPRVLDLKTIFLACGACLMTVTVLAAVLDDFS
                     GGTFNESGNWTLNQVIQSSNPSDTITYDQTTNAGKLTVTYVDEQGSDQITLTTWNGET
                     LDVGEFLQVKTAILAGGGVLVRPGIALATTTTPNARANTLLFGLRGDIVARAHAYRDG
                     GIEFSDPDAAITGYNAGDEVTLRIVRSSEREYQLFYGVEGPAETFVGAVTWPETGGTP
                     PSVPAILLDNGLASFTATFDDFEIGTAGAEPTPTPTPTPTPLPTPDAPNITIDDFNDS
                     SLDAAWETENYPQRDGEIDPITITEAGGKLTVEAEDAQGSAQVTLFLRNDVTLEPEDN
                     VQVDVRVVSGETTPLVRAGLGMAPTPSGIDGDRSNTLWFGIRNDGIMRGNAYGNAGAE
                     IGGVEPALDAYAPGTDVFTLAVQRLDENRYALWYGQNAPATVLAGTVNFAGATPPDVP
                     GLFFGVGLADLTLEFDNFRMFKKNLPNQAKTWSIYP"
     gene            complement(13259..13900)
                     /locus_tag="PLS90_06705"
     CDS             complement(13259..13900)
                     /locus_tag="PLS90_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02131.1"
                     /translation="MKLSSQRGFTLIELLIVVAIIAILAAIAVPNFLEAQVRSKTSRA
                     RADMRTMVTGSAAYRVDYNSDPTPDNVITSSDFDRNWWGFSSHLLTTPIPYLTSIPVD
                     AFPDFNDGSLRFGWPGNDFSIPYVVVVRANAKAPLGPKNFMWLGAPNVSSWPSLDPAS
                     RQQMQSAMSVYVSAGPDTQMTWQYAQVNSVLYPTAVYDPTNGTVSNGDLFAFE"
     gene            complement(14129..>15747)
                     /locus_tag="PLS90_06710"
     CDS             complement(14129..>15747)
                     /locus_tag="PLS90_06710"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK02132.1"
                     /translation="AGAAGGYDWAALISSNDASVKGGNFELRLTKTGQPMIAARNDQN
                     FYQQVGAPVAVTASPDRSLHIAGVYDINDHALYLYLDGELVDALPGFTASACPCEPQR
                     TIGISNSSFGSSFTGLIDAVAESTYTGQFTPADFVLIDAEGPAPSLPAEADRQVELVL
                     PPDVRDLPKAPGTRHVVYHPPDDQAMTYHHGAIVLPFKGRLVVAWQATLRDEHTPPYV
                     GLVSVSDDLTLANWSEPLEIGAPGNDAYLEYIGRRYNHPEGKSYLVNVAPRQMHATED
                     RLYLWCLAWTSEVGPEYGQASKDWASRIFWTEDGDNWHEIPPEELDTLENEKGLGSLR
                     SAGSNHQFTRLRDGRLMAYNLTDTGLWCPTTEDPTGLTGWGGGRIDSSDCADVGEPGG
                     WQDRDGVLHGTARWGQRIWHSYSTDGGQTWTKLTQQMKFPDCPGNKDFGMLPNGDVFY
                     VGNPVPGSRMELVLGLSHDGWTFDRNFLVRWEPHEQKWPARYKGDAGYQYPDAAEHDG
                     KLYVAYSVARDFIEVTAIDLDSLPDSPANE"
BASE COUNT         2709 a         5004 c         5312 g         2722 t
ORIGIN      
        1 gttcggctgg gggcaaccgg cgcagatgcc gcagttgtat gctggtttcg gcatccaggc
       61 gatcgtgacg gcgaagaatg tcagccgcca gcgcgccccc cattcggaat tcctcgggcg
      121 aacgccagag gaattccgaa tggggggcgc gctggcggct gaccgtcttc acgacgcggc
      181 tgggggagct ggggcgcgcc aacttctaca agcgcgccct gctcaagatc ctccatggcg
      241 gccagttctg gagcgacgaa tggcatttcg actggcggcg ggcggggctg atcgtgcgca
      301 gcgccgacgc cggcgatcag gccatggact tccatcatgt ggactcgggg ccgcggatcc
      361 acgccgaaca cctgcgcgac gccctgctca atgcgtggtc gaccaccgag gattcgctgt
      421 cgcccaccga ccggttcctg ggcgagggct gcgattatac cgtgggcctg gaactgctcc
      481 ccgagctgat cgagcaggcc aacgccgctt tcgatgaccg gcaattcatc cacggcaacc
      541 tgccggacta catccagcgg ctgcgcgaaa ccatctcgat ggatgagttg cgggtagtcg
      601 agggcgagct gcgcgatccg ccgataacgg cggtgcaggg caatgcgctc gccacgcggc
      661 tttatctgaa acggctcaac cgcctggcgg agcatgcgct gattcagggg gcggaaccgc
      721 tggttgccat ggcgcggatg atggggctcc cctgcccggc cggattcctc gaaaacgcct
      781 ggcgctacct gctgctgtcg cacagccacg atgcgatcaa tggcgtcacg ctggacaaga
      841 tctcgcgcga cgtcgtgcat cggctggagc aggtgctgga gatcgcgcac gccctcacga
      901 tccatgcgat gacggggatg gtcggccgga tgaacctgtc cgggttcgac gcgggggatg
      961 tgctgctggt ggtgttcaat ccgctgccga tggcgcggcg cgaagtgctg ttcgccgcgc
     1021 tcgacacgcc gcgcgagcag gatgtcgccc gcttctcgct cgtcgacacc gacggccggc
     1081 gccgccccgt gcaatggatc gatcgccacg agcacctggt cccggtgcat gacgcctgcg
     1141 cgcggccccg tccgttctat tgcgatcgtc acaccgtgta tttcgacacg ggcgaggttc
     1201 ccgccggcgg gtacaaggtc taccggctcg ttgccgagtc gcgccaggac cgctcgctgg
     1261 atttcctcta cgaacggatc gaccaagggt cgcaactgtg cgacggcggc atggggatgg
     1321 aaaacgaatt tcttaaggtc accttccaat ccgacgggac gtttgatctg ctggacaaga
     1381 ctacgaaccg cgcattccgg ggcctgaacg cttatgaaga tggcggggaa ctgggcgact
     1441 actggcaccg gacggttccg aatcacgatg aagtgatttc cagccggggc ttcccgcacc
     1501 ggattcgctt ggtgcgcgat gggccgctgg tgacgacgtt cgcgatcgag acgacgatgc
     1561 gggtgccggc gcgggcggac tggtccaaac gggcgcgcgg cgcggactgc atcgacctcc
     1621 ggatcgaaac ggaagtgagc ctggcggcgg ggcagcggcg cgtggatgtc acggtgcgat
     1681 tcaacaatca ggccgaggac catcggctcc gggcgctgat cccgacctgg atccaggccg
     1741 aacactccga cgcggaaggg cacttcctgg tcctgcggcg gccgatcacg ctggcgcgcg
     1801 acgtgaacgg cgttggcccg gggggaatga aaaccctgcc gcaacatacg tttgtcgatc
     1861 tgagcgatgg cgaggggggc ctggcgattc tcaatcgcgg gctgaccgaa ttcgaggtca
     1921 gcgaggacgc ctcgcgcacg atcgcgctga cgctgctccg caccgcgccg atgaaaatct
     1981 gcaccgagtt ccgcaccccc acggtcgacc cgaaccagaa gggggggcag tgcctgggcg
     2041 accacgtttg cgaatactcg ctctatccgc atacggggga ctggagccag gggggtgtga
     2101 accgcgaggc ccagctctat acggtcccgc tgcgctcgat gcaaaccgcc gcgcatgccc
     2161 gggggcacca cgcgccgacg cagagcttct acgaactgaa gtcggaccgg gtcgtcgcgg
     2221 cggcgatgaa ggcgcccgac gacgcgcaat caggctggga tctggtgctg cggatctaca
     2281 atccgtcgcc ggaggcggat cgcgcggaat ggacgttcca tcagccgctg gcgcgggccg
     2341 cgctgctcca gctcaatgag gaagagatcg caccgctgga ggtcacggat cagcgcaaag
     2401 tcggcctgga ggtcggaccg ggaaaaatcg tgacggtggg gattgcgttc cgggagtccg
     2461 agccttgagc tgaccgtttc cgattccgcg gccggaagcg ataacgattg cgataccgct
     2521 accgatagcg atggaagacc tttcccaaac cccttttttc agggcgtgga cttggtgaac
     2581 gaggtccact ccagcttgtc gaacgccagt ttccagtaat tgtgttcgtg catcgcctga
     2641 tagaaggcct cggcgttgcg ctgctcgagc gaggtgacga tgctctcgtg gagggcgcgc
     2701 acgtgatccg agagcgacgt ggtgtattgg ctcagggtct ggaaaaaggg attgagcaac
     2761 tgcgacatcc agatggcgat ctcattgccg gtgatcgcga tgagcttcag atgaaattgt
     2821 cggtcataat cattccagac cttgggctga tggccatgct cgaccatctt ctcgaaaatg
     2881 gcgcgtaact ccgcaatgtc ctggggcgtg gcgcggcgga agatctcgga gcacaggccc
     2941 agctcgagcg cgcagcgata cccgaatgcg tgccccatga agttgggcgg gatgttgggc
     3001 cgactcagca gccggatcag gtcgagcagg cgcggtgagc ggacgagcca catgccccgt
     3061 ttgcgccggg tatcgacgat gcccagggcg cgcaggcgcg taatggcctc gcgcacgctg
     3121 gagcggctca tctgggtctc gcgcatgatt tcgtcttcgg aaggcaacgg gtcgcccagc
     3181 tggacctcgt tgcggtcaat gtagtcctcc agccactgaa aaatctgttc gtaagcggcc
     3241 gcctcctggt tcggggaggc gtccggtttc gccgcgggtt ccctggcgct tgcctgtgcc
     3301 gatcgcatca tgcaagaagt tctctttcag tcagcaactc catcgatcac cccgtccccc
     3361 aagagcttat ccgagttaaa gtacactaac ggcgattggg cttcaatacc tgttatatgg
     3421 cgtgttaacc cgcgttgatg atttaagtcg ggacggaacc ggcgccccgg aaggcgcccg
     3481 gaaggggcgc cagagaccgg cgggaggatg tccgcgcgga ttggcccgcc cttcatggat
     3541 gattccgccg aaatcgcctc ggcgcgccgc atcatccccg tccggaaccc gctgcgccgg
     3601 cctcggccag cggagcaaag gcggatgcct gacggtccaa aagggcctga tattcatccg
     3661 tcttatccac cgcgcgcccg agcagggcaa aaccgcggag ccggccggcg ccgtcgcgca
     3721 ggagcgcctc cagatcctca tccgagccgc cgatctccca ggcgccctcg gcgccgccgg
     3781 gcggaagggc gatcaccacc gggcagcggg acgttttgac gacgatcggc atgacgcggg
     3841 agaaatcgac cggcgacggc gctcccagca gcgtgcgggc cagggacggc gcggcgtgtt
     3901 gaatcgggag gacaaacggc cgcacggcgc catcgaattc catgcagtcg ccaatggcat
     3961 agacgtcctc ggcggaggtc cggaggagcg aatcgacctg gatgccctga ttgaccgcga
     4021 ggccggccgc gcgggccagc tccgtattgg gacgcaggcc gatggccgac agcaccagat
     4081 cggcttccag ggttttgttc ccgtgcgtcg tgcagcggaa aggtctgtcg ccgcccggct
     4141 cgatggaaac gaggccttcg ccgaaatgcc actggacgcc aatcgattcc aatgcggtct
     4201 tgagcgcctg gcccagttgc ggcggaagga atcgggccag gggggcatcg gccagatcca
     4261 acacgcacgg ggcatagccc cccagggcga ggtcgttggc gaactcgcag ccgacgaggc
     4321 ccgccccgag aatgacaacc cttgcctggg gcgccagccg ctcgcggaag cggcgatagt
     4381 cgtcgagctg attgatgcac aggacctcgc cggcgagggg gccggccaag cgcggccgga
     4441 tcggttcggc gccgagcgca aggaccagct ttgaataggg gatttcccgc ccggggatgc
     4501 gcagccggcg ggcggcgaca tcgatggccg acacggtcgt ccgcgtcagc acatcggccc
     4561 tcagccgccg gctcatcgct tcggacgagg actgcaccag atcatcggga ttcttgcgct
     4621 gtgcaatcgc ctcggagagc gacggcttgt aataggattc gccgccgtcg cgggtgacga
     4681 tctgaatcgg cgcgtcgctg ttcagggcgc gcagctcgcg cgccaccgcg tagccggcca
     4741 agccgctgcc gacgatgacg atcggatcgg cgagcgaatc aatctcgatc cgcggcttgg
     4801 cggccgccgc ctcgcggcgg atctcgacca tcttgaactg agacttccgc acgccgcaga
     4861 cggggcagac ccagtcttcg ggtatgtcct cccagcgcgt gccgggagga aacccttcct
     4921 cggggagccc ttcgtgctcg tcatagatga agccgcagag ttgacactcc cagacgcggg
     4981 cgacctcggc caggccggcg tccgacgccc cgccttgcgt cgcctgaacc ggcgccgtct
     5041 ccggctcgcc gggcaggcgc gcgcgaaact gatagctcgg atcgtatttc tgcttgatga
     5101 ggatgtcggc gatctggacc caggtctcga tcagcccgtt ggggcagtgc ggcagctcgt
     5161 gcttgatcgc ctcgtgcagg cgggggagat gatagcacgg gacggcgggg tacatatggt
     5221 gctcgatatg ataattcatc cgccagtaga ggaagcgcag gaagggattc aggtaatacg
     5281 tccggcagga gagacgaaag tcgttgacct cgtcgaccag cccgatgtgc tggggcagcc
     5341 ccatcagcat gatgagtccg ccgccgtagg cccggcccag cgaaacaacg acgggcagaa
     5401 tccagagacc gctgagcgcc ccggcgacca atacggcggc atggcccagc aacgccaggc
     5461 gcgaccagtt gaagacccgc cggcgcgtga cggcgtccgc ccccgcgatc agttgcgtat
     5521 accacgtccc ccgcggccgc cccagcgcca gcccgacgtt gtcgacaatc cgcttgccga
     5581 ggacgcggag atccagaaaa ttcaccagga tcaactggag cagcgtggcc ttctgcggca
     5641 gcacgacttc caggtcgtcg ggctggtgca aggtgaactt gtggtgctga ttgtggctca
     5701 gcatgtagag ccgcggattg tcccaggtga ggaagcagac gacattgagg aagagccggt
     5761 tgagccagcc ggtgcggaag acgcgctcgt gactgagttc atgggacgcg gcgccgaacg
     5821 tggagaccac ggtgccgtgg agcagcaggg cggggatcag ccaccacggc gacaggttga
     5881 ggtaaacaag gacggccgtg aggccggtga cgaaggcgag cgcgacgtgc ccgccggcct
     5941 ggaggaagcc ctgaaggtcg ttgggcgcgc tcagctcgcg cagcaggtcg ggggcgacct
     6001 taactcgata ccagtcgatg cgctgattcg attccatggc ggttatcctc ggaagacggt
     6061 cggtcggcat cggccggcgc ggcaagtcgg gccgccagct cgatcgcgga gatcaggctc
     6121 gagggatccg ccttgccctg ccccgcgatg tcgtaggccg tgccgtgatc gacggaggtg
     6181 cggatgatcg gaaggcccat ggtgatattc acgccgtcat ggagcgaaat catcttgaac
     6241 gggatggagc cctggtcgtg gtacagcgtg acgtggccat cgaagcgctg cagtgcgcgc
     6301 ggcatgaacg cggcgtcggg ggggatcggc ccttcgacgt tccagccttc ggcgcgggcg
     6361 tgctcgacgg cggggcgcac gatccgctca tcctcgtcgc cgaacatgcc gccttcgccg
     6421 gcgtgcgggt tgagccccag cacggcgagc cgcggctcgg cgccgcgaat ccgccggagc
     6481 gtctccgccg tcagatgaat cagccgctcg atctcctccg tcctgagggc gtcgggcacg
     6541 ctgcgcagcg actgatgaca ggtgacgaag ctgacagcca ggcgcgggct gtagagcatc
     6601 atggcgaaat cgcgcgagcc ggtgaggcgg ccgaagattt cggtgtgacc cggatcgtcg
     6661 atccccgcca ttttgagggt ctgtttggtg atcggcgcag tggtgacggc gtccagcttg
     6721 cgcgcgagcg ccaggcgaat ggcgttctcg atgtaggcat aggacgcgcg gccgccgtca
     6781 cgggtcggcg tccccggggc gacgggcgcc gtcaggcagg cgcagtcgac gacgagtgga
     6841 ccgtcgggca ggtcgacacg cgccgtgcgc tcgaattccg ccagcgtcag cgccgtcgcg
     6901 gacagcgggc gaccgagccg ccgagccgca tcctccagca caacccggtc gccaagaatc
     6961 agcggtcggc aaaatccgag aacggacggt tcgtgggtca gcttcacgca cagttccggt
     7021 ccgatgcccg ctggatcgcc ctgcgtgatc gccacaagcg gcttgactgg gttggtcacg
     7081 gcttgtctgg atccctttcc tgtgtgattg aactgtgcgt cgcgccgacg gtcaatcgac
     7141 ggcgggcgtc gtcgtcagtt gcgaccactc gcgcagcttc atgatcaact cgtcgttctc
     7201 gtgccaacgg aacccgcgcc acaggccatc gaccgaaacg atggcgggac gcgcgacgtc
     7261 gaagaagcga tcgatcaccg cgatgtcgcc ggcggaaaag ccgttggcca cgtgccagcg
     7321 gcaagccgtc tggatacgca gacccaggcg ctggagcatg tatttcgccg aggcggggta
     7381 cttcgtctga ttcatgatgt tcccgatgct cagcatcagt tcgcgcgcct gatggtgcca
     7441 cggcgtggtg cgttgcggct cgagcagata gtcgcggctc aggcgggggg cgacattgac
     7501 cagcgttccg gcgaagccgg cgccggtctg ctcgcccccc tccagcaggc aagccaggtt
     7561 cgcctggaag acctgcatcg gcgtatcgcg cgcgagctcg accttctggc tgaagaccgg
     7621 cacctggcgg ctcgtctcct tcaggaagac gaaacgcccg gtggcggcca gttcggcgac
     7681 ttcccccggt tggagcgtgc gatggacggg gatcggcgcc tcgtagatgc cgagcggcat
     7741 ctcgccgccg tccatcagca gccggatctc atcgtggatg ggccgcgcgg ccggcaacat
     7801 cgaggtcgag atcacgacgg cgtccagatc ggccgtggcg gccaaccggc gcatcgactc
     7861 gcgttgctgc tcgagcgtgt caccgatgtt gccggtcgcc acgacgccga tgcgaccggc
     7921 ggcgcgctcg gtgatccgcc gcgcgatctg aagcatctcc tccggttgga ggtcgaagat
     7981 ttcgctcgag gtgcagttcg cgaagacgcc ggcgacgcct tccgcgatgt agtagtcgat
     8041 catttcatcg agcgccgccc agtcaatcga caggtcatcc ccgaacgggg tcaggagaac
     8101 gggccagact ccctcgcgca cgccagttcg agttttcatc gcaacacccc ctcggcggtc
     8161 tggccggcac ggtaaagaat gggacagacg ctttcatccg acaatttgcc gacgtaatcg
     8221 tagaccctgg cccgaccgtg gatcggagtg gcgcggttcg tgcgcagatg gatggcgaaa
     8281 ctcatccgcg ggccgttgga ccggttgggg tagctgccgt ggacggtgta cttgtgatgg
     8341 acgctgaacg ccccggcggg cattggcgcg gtcacctctt cccacgtcgc accgtcggga
     8401 agcgggattg actggcggct ctggctgtcg ggcttctcga agaagctgcc gccgggccgc
     8461 aaaccccacc gatgcgagcc gcggacaaaa cacagcggcc cgctctcctc ggcgacgtcg
     8521 ctgatcgcca gccaggcggt caacacctcg ctgtcgggcg tccagtgatc ctgccagtag
     8581 gccatgtcct ggtgccagcc gacggaggcg gcctcgctgc ccgccggctt gtagaggagc
     8641 tggacggccc acgcctgaac cagctccgcg ccggtcagcg cggcgatgta ctcgccgatc
     8701 tcgggcttgg aaataatgcg atggatcgtc cggttggcga cctggggctg atcgatcttg
     8761 cacaggctga ggcgcggatc gtcgtccggt ttgcgaaacg cgtcgcgggg ggcgaggccg
     8821 gtctcgtagc gccccgccat tacgtcttcc atcgcctggc gcgcctgccg cagttcgagt
     8881 tcggtgagga tgggttggac gacgatgaag ccatcccgtt cataacgctg ccgctcatcg
     8941 agacggccat tgtggttcac gattgtcatc ttgtcaggtc cattccgagt tagttcgcga
     9001 aagctgttgc ctggcaacag ggcttttcct atcactcaat gcacatcagt ccggtgcgtt
     9061 cgccgccggg ctttcgccgg cctcagccag cttttcaagg gggatttcga gcacctccac
     9121 gtcttccttg ttgacggaat aaatgatcca cagggaaccg ttcgcaatca ccgcgtcggg
     9181 gtactggaac cccgggatcc agcccaggcc gttgaaccgg gcattgggcg tgtcgtggcg
     9241 aatcgcccag gcgcggtcga atccgcgacc gtcccggctc aaggcgagga cgagcggatc
     9301 gcgcgggaag tgttcggatt ggaaatcccg ttccgcaaac ggccagacct tcgtcgggtt
     9361 gcggtacggc cgcgcgtcgg gatcatccag ttcccccgcg atctgattgc cgatcagata
     9421 gacggtgccg tcgggcagct cgccgacggt cacgccggag ggcgaatcgg gaatgttcgt
     9481 cggcacggcc agcgaccacg cctcggtgtc gctgtggcgg gtggagacat aaatgcggtg
     9541 ggttccgttg taattccgcc agacgcgcac ctgcatgccg tcgggactga cgtagatcgg
     9601 gtcgtggctg atgatgctga agtccacggc ctccggtcgg gcgccgcgat agatgtcgcg
     9661 gggctccacg gagcgatcca tgaagtcgtc gggcaaccgg agctgttgca gcagggcctg
     9721 cccatcggcc cggagttgtt catgggtcac gttggccagg ccgggatact gctcccctcc
     9781 cctgggcgca tgctcgttca gccagaaaac cggacccatt tcgtccacac ccaggatttg
     9841 gcgggccagg aagcccatct ggccggactg ccccaggtag ccgccgcggt aacccgcccg
     9901 gcccagcacg tagacgcggt cctccacggc caggaagccg ccaaactcat tgggccagat
     9961 ggaaacgccc ggcttgtgcg gatcctccag atcatcggcg gggtcgctca gggaaggcag
    10021 catgacgaac gacggggacc atgtcttccc cccatccagc gagatgctgc cgcggacgcg
    10081 cgttccggcc gaatcctcgt gcatgtagct gttgctccag cttccgtaga ggcgcccgcc
    10141 gaacgccgcg atcttggcgt gatgacagaa cgtccagcct tcggggttcg cgcgatagag
    10201 cgtgatcggc tcgagccccg gcatgacggg gtagccgcca tcggtccgat acgggtccgg
    10261 atgcgcgaca tcccagcgaa cggggggatc gtcgccgagc gcccagcgcg aagcgatgtc
    10321 ggccggcagc gcctgggcgt tgaattccgt cagttcgacc gaacaaccgc gcagattgag
    10381 gccgagctcg ccgccgcggc cggcgctgcc cgcgatgcgg tgacgcagga cgagattttc
    10441 catgccgttg acgcgcaggg tgatctcgtc gccgcgcgag gcagcgatcc attcctgctc
    10501 ggcgcggtcc ttcgcccggc cggcctgtcc gtccagatcc atccgggatt gctcgagcgc
    10561 ggcctgcgcc tcggtgaaat ccgccagcgg cgagaccccg gcccaatccg tgaccggccg
    10621 gtgatcgatc tcccagaccg agcggccgtt ttcgcgaacc tgccaaatca cctgatattg
    10681 gccggtctcc gcgttcagat ggccggaata gccgacttcc accccatcct tcagggcgat
    10741 gaaacattcg accggacgac cgagcgcgcc gagcgacttg aatcgcatct tgaccagccg
    10801 gtcaccggtt tcgggcgcgg cgacgcgcaa cgtggccgcc tgaaacgccg gcgccgagag
    10861 caacagtcgc tccgcggaat cctgagcctg aagcatgccg tcgacgcgcc aggcgtcggc
    10921 gccggcaccg cccggcccgg tcacttcgcg gccgatggtt tcgggaggcg gcagcatctt
    10981 gacgccacgt tgcaacgcga gcccccaccc gtcgcgcccc gccgcgagtc gaaatccgag
    11041 ggtattctgt ccggactgga gcggaacttc aaaacgatgc aggccccacg tttcgccgtc
    11101 gttgccgccg gcataggcca gccggccgtt gacccagact ttgagccagc cgcgcgcccg
    11161 cgcctcgacg ggcaatagcg cgtcgccgtc gctttgctcc tgccggtaga ggtagaggag
    11221 gccctgaccg gcgttggtat gccacatccg ctggaggtcg agctcctgcc cgtcgtaaat
    11281 gagctgacgg ccctgctcct ggacgccatc gacgtcggcc tgctccagga actccgggct
    11341 gccgtccagc gccgtcggtt cgagacgcgg ctcgaaccac ggccgccatt tgccgggatc
    11401 cttctggctg gcgatggggc cgaacatgcg ccatgcgccg cgccagggca tcatctcggg
    11461 tgaatcgtta ttctcccgcg caccgccggg gatcgaaagc agcgcaaaca atcccgccag
    11521 aaggaatccg cgagaaaggt ggtggcgcgg cgagtggcgt cgccgcagcg gtcgcccgac
    11581 agcactggta tccagcttca cgcttgcctc ccgttcacgt ttctttcaaa tccggttaag
    11641 ttggttgatc cgcccgcccg ggcggggtgc ggagcggcga agccgttcgt cgctccgcac
    11701 caccggccat gggtcttgac ttccgaggtt caaggataga tcgaccaggt cttcgcctgg
    11761 ttgggcaggt tcttcttgaa cattcggaag ttgtcgaact ccagcgtgag gtcggccaaa
    11821 ccgacgccga agaacagacc gggaacgtcg ggcggcgtgg cgccggcgaa attcaccgtg
    11881 ccggccagga ccgtcgccgg cgcgttctgg ccataccaga gggcatagcg attttcgtcg
    11941 agccgctgca cggccagggt gaagacatcg gtccccggag cgtaggcatc gagcgcgggc
    12001 tcgacgccgc cgatttccgc gcccgcattg ccgtaggcgt tgccgcgcat gatgccatcg
    12061 ttgcggatgc cgaaccacag cgtgttggag cgatcgccgt cgatgccgga gggggtcggc
    12121 gccatgccga gtccggcgcg gaccagcggg gtcgtttcac ccgagacgac ccgcacatcc
    12181 acctgcacgt tgtcctcggg ctcgagcgtc acgtcgttgc gcaggaagag cgtcacctgg
    12241 gcgctcccct gggcatcctc ggcctcaacc gtgagtttgc cgccggcctc ggtgatggtg
    12301 atcggatcga tttcgccgtc gcgctggggg taattttcgg tctcccaggc ggcgtcgagc
    12361 gaggaatcat tgaaatcgtc gatcgtgatg ttgggcgcgt cgggggtggg cagcggcgtc
    12421 ggcgtggggg tcggggtcgg agttggctcg gcgccggccg tgccgatttc gaagtcgtcg
    12481 aaagtggccg tgaaactggc cagaccgttg tccagcagga tcgcgggcac cgacggcggg
    12541 gtgccgccgg tctcgggcca ggtcacggcg ccgacgaaag tctcggccgg tccctcgacg
    12601 ccatagaaga gttgatactc gcgttcggac gagcggacga tccgcagcgt gacctcgtcg
    12661 ccggcgttat accccgtgat cgcggcgtcg gggtcgctga actcgattcc cccatcgcga
    12721 taggcatgcg cacgcgcgac gatgtcgccg cgcaagccga acagcagcgt gttggcgcgc
    12781 gcgttcggag tcgtcgtggt cgccagggcg atccccgggc ggacaagcac gccgccgccg
    12841 gccagaatcg ccgtcttgac ctgcagaaac tcgcccacgt ccagcgtttc gccgttccag
    12901 gtggtcagcg tgatctgatc actcccctgc tcgtccacgt aggtgacggt cagcttgccc
    12961 gcgttcgtcg tctggtcata ggtaatcgtg tcggaggggt tgctcgactg gatcacctga
    13021 ttgagcgtcc aattgccgct ttcgttgaac gtgccgcccg aaaaatcgtc cagcacggcc
    13081 gcgagcaccg tgaccgtcat caggcaggcg ccgcacgcca ggaaaatcgt cttcaaatcg
    13141 agcacccggg gcttgtcccc ctggcgcaaa gaatgctttc ccttatgcat gatctctcct
    13201 gtttcattaa ggtttcaaac atcgaccttc gattgatccc gccagcccgc ggtcgttgtc
    13261 attcaaacgc gaacagatcg ccgttggaga ccgttccatt ggtcggatcg tagaccgccg
    13321 tggggtagag cacggagttg acctgcgcgt attgccaggt catctgcgta tcgggacccg
    13381 ccgagacgta gaccgacatg gcgctttgca tctgttggcg cgaggccggg tcgagcgagg
    13441 gccaactgga gacgttcggc gcaccgagcc acatgaagtt cttcgggccc agcggcgcct
    13501 tggcattcgc ccgcacgacg acgacatatg gaatcgagaa gtcattgccc ggccagccaa
    13561 aacgcaacga gccgtcgttg aaatcgggga aggcgtcaac cgggatcgag gtgagatacg
    13621 ggatcggggt cgtcaacagg tgcgagctga atccccacca attgcggtcg aagtcgctgg
    13681 acgtaatgac attatcgggc gtcggatcgc tgttgtaatc gacccgatag gccgccgagc
    13741 cggtcaccat cgtgcgcatg tcggcgcgcg cccggctcgt cttggaacga acctgggctt
    13801 ccaggaaatt cgggacggca atcgccgcca gaatcgcgat gatcgccacc acgatcagca
    13861 gctcgatcaa cgtaaagccc cgctgagagc ttagcttcat gctctttatc ctcctcgttt
    13921 cgctacttag gtcatcgatg tgccggaagc gacggccgac tgcactcgag cgaccgacgg
    13981 gcaggctata gacggtccga caagaacgac ttcgagtgcg gtttgcgtct cggcgatttt
    14041 ccgccggagg cgatcatccc gtcgaaaccc gcggccagcc ggtcctgccc acgttcaact
    14101 ggggatcggc cctcctttcc gcgagcgctc attcgttcgc cggggaatcc ggcaaactgt
    14161 ccagatcgat cgccgtgact tcgatgaagt cgcgcgcgac actgtaggcc acgtagagct
    14221 tgccgtcgtg ttcggccgcg tcgggatact ggtaacccgc gtcgcccttg taacgcgccg
    14281 gccatttctg ctcgtgcggt tcccagcgca ccagaaagtt gcggtcgaac gtccaaccgt
    14341 cgtggctgag gccgaggacg agctccatcc ggctgcccgg caccggattg ccgacataga
    14401 acacgtcgcc gttgggcagc atgccgaaat ccttgttgcc ggggcagtcg ggaaacttca
    14461 tctgctgggt cagtttggtc caggtctggc cgccgtcggt gctgtaggag tgccagatcc
    14521 gctgacccca gcgggcggtg ccgtgcagga cgccgtcgcg gtcctgccat ccgcccggct
    14581 cgccgacgtc ggcgcaatcg gagctgtcga ttcgcccgcc gccccaaccc gtcagccccg
    14641 tggggtcctc cgtcgtcggg caccacaggc cggtatccgt gaggttatag gccatcaggc
    14701 ggccgtcgcg aaggcgggtg aactgatgat tgctgccggc cgagcggagc gaccccagcc
    14761 ctttttcgtt ttccagcgtg tcgagttcct cgggggggat ttcgtgccag ttatcgccgt
    14821 cctcggtcca gaagatccga ctggcccagt ccttggaggc ctgcccgtat tccggaccga
    14881 cctcgctggt ccaggccagg caccacagat aaagccgatc ctcggtggca tgcatctgcc
    14941 gcggggcgac gttgacgagg taggacttgc cctccggatg attgtaacgg cggccgatat
    15001 actccaggta ggcgtcgttg cccggcgcgc cgatttccag cggctcgctc cagttcgcca
    15061 gcgtcagatc gtcggatacc gagaccaggc cgacataggg gggcgtatgc tcgtcgcgca
    15121 gcgtggcctg ccaggcgacc accaggcggc ccttgaacgg cagaacgatc gcgccgtgat
    15181 ggtaggtcat cgcctggtcg tcgggcgggt gataaacgac gtggcgcgtc ccgggggcct
    15241 tgggcagatc gcgcacatcg ggcggcagga ccagttccac ctgtcggtcg gcttcggccg
    15301 ggagcgacgg ggccgggcct tcggcgtcga tcagaacgaa gtcggccggc gtgaactgac
    15361 ccgtgtaggt cgattcggcc accgcgtcga tcaggcccgt aaacgagctg ccgaacgagc
    15421 tgttcgaaat gccgattgta cgttggggct cgcaggggca ggcgctcgcc gtgaaaccgg
    15481 gcaaggcgtc gaccagttcg ccgtccaggt agaggtagag cgcgtgatcg ttgatgtcgt
    15541 agacgccggc gatgtgcagc gagcggtcgg gggaagcagt cacggcgacc ggcgcgccga
    15601 cctgctgata gaagttctgg tcgttgcgcg cggcaatcat cggctggccg gtcttcgtca
    15661 ggcgcagttc gaaattgccc cccttgaccg acgcatcatt gctggagatc aaagcggccc
    15721 agtcgtagcc gccggcggcg ccggcgg
//
LOCUS       DAOPCO010000066        15708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3812_length_15708_cov_5.603143, whole genome shotgun sequence.
ACCESSION   DAOPCO010000066 DAOPCO010000000
VERSION     DAOPCO010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3812_length_15708_cov_5.603143"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLS90_06715"
     CDS             complement(<1..1293)
                     /locus_tag="PLS90_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02133.1"
                     /translation="MCRLLLVVLLFGLPGLITRTWAQSIRDEQLPQFVQGASGVNNNR
                     IPFYCRLSLTGLTPGATYAYYHRCVDAADPTTTTLFGNMLLLDPSGPIARIATPGFTP
                     GRHGVFTADAAGAYSGWFGIEPTAHRGFAAGRQIRLRLHLNDGAGGAATAHRLTSAGA
                     LTVLDWATTRTGPGGTGLVGHSSFAPRSVVLLYGDDAPDARPLASAMVEDDGLDCPDS
                     SYVNFHDELVDGALGAWGTIIPNALPAGVRRIEAHAFDDGRLLQSLHSADGTWPPAAT
                     DTKNPAGGHIRPIVIRIGGDDPNIEVVPAELLLSAAAEHTTATGTVWIRNTGAAESLT
                     VTGAVIDGPGAARFAVHGELPPAIASASSAPLTLTYQPRGSLAPTTATLRIASNDGGG
                     DVPAITLRGSAAPGPAAYEGLLIAEIATRPTADEFIEIV"
     gene            complement(1405..2475)
                     /gene="tilS"
                     /locus_tag="PLS90_06720"
     CDS             complement(1405..2475)
                     /gene="tilS"
                     /locus_tag="PLS90_06720"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPK02134.1"
                     /translation="MARKRTTPAPPVSSSPEEETAARVTDGFRSACDRLAPLAAGAGL
                     LLGCSAGGDSMSLLELAAAEAPRRDWRLAVVHVDHAQRPESADEARFVHDRAAALGLT
                     CFIERLPESLVAAGPLSEDVLRAARLRCYVRAAEAWRADALVLAHQADDRAETFLMRL
                     LAGSGPTGLAGIRPIDAVEGLTVVRPLLALRRADLRRFLTARGLTWRDDPTNASEETR
                     RGWLRHAVIPLLRDRLGIDPTERIVRAAELIEEEARALDDAVARLLELLRCQAEPPAV
                     AALDLGRPAWRKSGRLLRRQLLRAWLWDLRRGPHPPGLAAVEEALTFAERGRRDARLR
                     TIERWHLIRRGRKLEAIPPEDA"
     gene            2725..3102
                     /locus_tag="PLS90_06725"
     CDS             2725..3102
                     /locus_tag="PLS90_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007510435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPK02135.1"
                     /translation="MPANPCLQCGACCAFYRASFYWAEADDATPGGVPAELTTAHPPH
                     FRVMRGTEGPNPRCVALAGTIGEAVHCTIHPRRASVCRDFVPSWAEGERNERCDRARA
                     AHGLPPLRPEDWISPDDDLEPAA"
     gene            complement(3136..3567)
                     /locus_tag="PLS90_06730"
     CDS             complement(3136..3567)
                     /locus_tag="PLS90_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015230878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK02136.1"
                     /translation="MSQAKLGDLVRVHYTGKLDDGQVFDTSRGREPLEFRLGDGQIIP
                     GFEQAVVGMTPGDHQIVRIPHEEAYGPYHEEMVQQIDRSAIPESIPLELGMQLKASRD
                     GQDPLVLTVVEIEETLVTVDANHPLAGQDLTFDIELVEIAG"
     gene            4280..5464
                     /locus_tag="PLS90_06735"
     CDS             4280..5464
                     /locus_tag="PLS90_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02137.1"
                     /translation="MRDLTQQGGVLGGTTVLLAALLGVGLWMLMDLPDSEAQPATSSP
                     GTLPEDIFDLDSEQIILEDFQTEITEQDETQHRISASWARLNESDEIIDMKGLSVEFY
                     SDNRPRGEATSGYGRIWLEDSEEDGRSRNDLLLSETVRYYTIKQEDDEEAAQQGEWVL
                     ETPLMLFTSDDSTLRSDKGYVKQMPMESGALGSVFSGEYFDRVRADRIQYDAQGSVAM
                     RGNVRYESQALGIVGQSLAVDTASKVIQGAGDPLWLHRGVDMQAQCGKFVYDTEAGRI
                     QLTEEPVVYQLAEGKVQRIRAGVVNMMEDESGRLGVSLRGGPGGRFEIDRIDEVPDSP
                     IVLPGSAPKPAEAAADSAGQFLVGRGERFEIKVNLAANTFDTWTEYTAILEKTEQPVI
                     LP"
     gene            5461..6066
                     /locus_tag="PLS90_06740"
     CDS             5461..6066
                     /locus_tag="PLS90_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02138.1"
                     /translation="MKRCLLIIPTCLLLAAVVVPAVGAQEEEGSAGLGSFAGMDFDKI
                     RAHQLDLSPDGRINLVGEVDIESETLAIKADVVTSDETGDVLKAKGSPVRIRQGDTVR
                     AECRDFTYYVESGKSILEGSPVIYQTTEEGTTKVVGGIIILTKDEEGNTGISVKMDPG
                     SKTMPTIENIKSAPKASAPQEKKPATKVNDSPGLIRLPALE"
     gene            6287..7240
                     /locus_tag="PLS90_06745"
     CDS             6287..7240
                     /locus_tag="PLS90_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK02139.1"
                     /translation="MKILVTGAAGFIGSHLAERLAAAGHEVVGIDCFTEYYSTALKRL
                     NAEDVRSHGVTIHELDLAADDLSRAVRGVEAVIHLAAQPGISDHVSFQTYVRNNMEAT
                     WRLLEAVKSQDSLRGFINVATSSVYGAHATDDEETAPKPTSYYGVTKLAAEQLVLAYH
                     RDKALPACSLRIFSVYGERERPEKLYPKLISSILEGREFPLHRGSDRHSRTFTYISDV
                     LDAFEAVLARLDACNGEILNIGSDIEITTGEGIALIEKIMGRRARLAIKPPRAGDQLK
                     TRANIEKARRLLGWEPRVPPEEGLRREVEWYENRIYGRVAI"
     gene            7392..7994
                     /locus_tag="PLS90_06750"
     CDS             7392..7994
                     /locus_tag="PLS90_06750"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK02140.1"
                     /translation="MVERVPNSARAQLGYARQLAERDDLEGAIAHLRQSLRIYPTRIE
                     AVARLGLLLAEVGREDEALDPLYRAHLADPGRLELTDVLAGILTRRGEFERAEAVLRR
                     SLEARPTFLRIQYDYGRWLAERGRYREARPLLEQALPRLPDDHRARVLLPLAEGYLAA
                     GDAARAEAMVRAWLEVRPADPDGAAAALLERIAAARQPEG"
     gene            8051..8599
                     /locus_tag="PLS90_06755"
     CDS             8051..8599
                     /locus_tag="PLS90_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02141.1"
                     /translation="MCGANIPRMKALEAHPVAAAGALTVALAAVFELTEISVVFLLGL
                     MGIILFVGLVWGPRLPLFVRSWFTWMIFYFGALACMFGRTMAPGLTLYTAGMALLSFS
                     SMALLPALALLRLWHGRRRLALIGLVLPVSLLLGCVVGFTEERLFIWRYRETGAGPTP
                     RWTVEMHWLAYDPATGELAGSD"
     gene            9075..10436
                     /locus_tag="PLS90_06760"
     CDS             9075..10436
                     /locus_tag="PLS90_06760"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPK02142.1"
                     /translation="MTATMNTWWLFIGHEEIKIELKQLEDEGRDPAPVKAGLERLLKL
                     SGDELRLPKNQEKAGELFDRARTLKMRKGYEFDEPSDLKSIRKLRPRGPRKYARRLPD
                     KVLLDRITAAWTARCCGCLLGRAPIEGARSWEMEGFLKMTGQWPLRDYFRFGVRGKAA
                     REYPKFTAGAHLDTIDHMPVDDDTNYTTTGFLLVKQRGADFAPRDAADFWMMNIPLLA
                     TCTAERVAYRNFAMNLAPPDSATFRNPFREWIGAQIRADAFGYLNAGDPQRAAEFGWR
                     DASISHVKNGIYGEMWAAAMIAAAPYCETFEEIIRVGLSEIPRTSRLHAAVSEVLDWR
                     KRGLTYEAAVQKIHDRWDEQFHHDWCHTITNAVIVAVGLLWGEDDFGTSICRAVTPGY
                     DTDCNAATIGSVFGMRYGTEALPEKWTRKINNTLKTSLVGYETVRLDQIARETWELAR
                     KIR"
     gene            10716..12068
                     /locus_tag="PLS90_06765"
     CDS             10716..12068
                     /locus_tag="PLS90_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPK02143.1"
                     /translation="MDEKKIILPERELPTHWYNLAADLPEPLQPPRHPATHAPLTPAE
                     LAPLFAEALIEQELSTERWIEIPTAVREAYRVWRPSPLFRATGLEAALETPAEIWCKW
                     EGVSPAGSHKPNSAIPQAYYNAREGIRRLTTETGAGQWGSSLAYACAQFGLECTVYMV
                     RISFSQKPYRASLMRAWGATVFPSPSDQTGSGRAALAEDPETNGSLGIAISEAVEDAV
                     AHDDAHYALGSVLNHVLLHQTIIGLETQKQFKRAGREPDVLVGCVGGGSNFAGFVFPY
                     IPEKLGGHNLRLVAVEPAACPTLTRGRLAYDYGDTAGLTPLIRMHTLGSHFMPPGFHA
                     GGLRYHGMAPMVSAAVEQGLVEPVAIPQLETFEAGLLFARTEGIMPAPEACHAIRAAI
                     IEAERCKAERKKEIIAVNVSGHGHMDMAAFDAYLSGKLVNQEHDEQALALSLSHLPAI
                     "
     gene            complement(12136..15639)
                     /gene="mfd"
                     /locus_tag="PLS90_06770"
     CDS             complement(12136..15639)
                     /gene="mfd"
                     /locus_tag="PLS90_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPK02144.1"
                     /translation="MLSHFLERLAVEPATAEIVECAARADDKLVVTGARAGAAPALLA
                     LLARRLGRTMLVVTSSLERAEALTDGLAFFGARPLIYPSFETLPFEETEPVLHIAAAR
                     NAVLAELAHLDPEAPQAAPLIVAPVDALAFRPLPREALLRQSLTIRWGQTLDPEALAA
                     QLVGMGFRREALVESPGEFAIRGSIIDVYPPDASNPWRLDLFGEEIDQIRAFDPQTQR
                     SLPRDAETEAVRILAPGCVEPKLAWLRAGHALGSLFDLLPENSLVVLDGPFRLDQRLR
                     RFDEIATRHWGDIQRIQHNDQVSFFVEHNLTPGQWLLSRTAVQEALTSFQSLDLTDLG
                     SDIEEEEDNRPAEEALAGRRLTFPAQGFESVTSQFQNYLGLIRERLRKGHWVAIVCDN
                     NGQVMRLDELLRENEISALPLVDGQPAPALPRSAADRCPDVLLMVGDLHEGFHSPQAG
                     IFIVTDREIFGRYKRRHVYRKAAARGKAISNPTEIQRGDYVVHIEHGIAHFERIRRQN
                     VDGRLTEFLELVYQNDDRLLVPVDKLHLIQKYASADGKPPTLDRLGSRKWQSRRRKSM
                     EAVRKMAGELLELYARREAAEGYAYAPDSAWQQEFEASFIYQETPDQLEAIRQTKANM
                     CEPRPMDRLVCGDVGYGKTEVAIRAAFKAMVEKRQVALLAPTTLLVQQHYNTFSERLA
                     DYPFKVEMISRFRSTAQINEILRQLKEGEIDLVIGTHRLLSRDVQFRDLGLLIVDEEQ
                     RFGVAQKEKIKNLRANVDILTLTATPIPRTLYMALSGLRDLSIITTPPANRHPIKTRT
                     IRFDREQIEEAILRELNRGGQVFFVHNRIETIQDVAQTIREIVPRARIVVAHGQMDEQ
                     DLERIMLEFIEGKYDILVSTTIIENGIDIPNVNTIIINRADTFGLAQLYQLRGRVGRD
                     VRQAYAYLILPPGEAITPQAIKRLEALEEFTDLGVGFSIAMRDMEIRGTGNILGREQH
                     GAITDVGFEMYCQLLEEAVSELRGVGAREPLWPVDVKWPVEQFLPEEYIPIESQRIRF
                     YKDIAAAREKDELDLLLEELVDRYGALPEPAINLINACRLKLAAAPWRVDRIQPYTDD
                     TVRIKAPVFAAELAAALAANAAAGRQVFSRLRRSGDQIILALRETDEEWLDERTLAAT
                     ADYFEALPAPPVVAENSAISA"
BASE COUNT         2561 a         4959 c         5728 g         2460 t
ORIGIN      
        1 gacgatttcg atgaactcgt cggccgtcgg gcgggtggcg atttccgcga tgagcaagcc
       61 ctcgtaagcc gccgggccgg gagcggccga cccgcgcagc gtgatggcgg ggacgtcgcc
      121 gccgccgtcg ttgctggcga tgcgcaacgt cgccgtggtg ggcgcgaggg aaccgcgggg
      181 ctgatacgtc agcgtgagcg gggccgagga ggcggacgcg atcgcgggcg gcaactcgcc
      241 gtgcacggcg aaccgcgcgg cccccggccc atcgatcacg gcgccggtga cggtcagcga
      301 ttcggcggcg cccgtgttgc ggatccagac cgtgccggtg gcggtcgtgt gttccgcggc
      361 ggcggacagc agaagctcgg cgggcacgac ttcgatgttg gggtcgtcgc cgccgatccg
      421 gatgacgatc gggcgaatgt gtccgccggc ggggttcttc gtgtcggttg ccgccggggg
      481 ccaggttccg tccgcggaat gcagagactg gaggaggcgg ccgtcgtcga acgcgtgggc
      541 ctcgatgcgc cggacgcccg ccggcagcgc attggggatg atcgtgcccc acgcgccaag
      601 cgctccgtcc accaactcgt cgtggaagtt cacgtaggag ctgtcggggc aatccagccc
      661 gtcgtcctcg accatcgcgc tggcgagggg ccgcgcatcg ggggcatcgt cgccatagag
      721 caggacgacg ctgcgcggcg cgaaggacga atggccgacg agcccggtgc cgccggggcc
      781 ggtccgggtc gtggcccagt ccagcaccgt gagcgcgccg gcggaagtca ggcggtgagc
      841 cgtcgcggcg ccgcccgcgc catcgttcag gtgcaggcgg aggcggatct ggcggccggc
      901 cgcgaagccg cgatgcgcgg tgggttcgat gccgaaccag ccgctgtagg cgccggcggc
      961 gtcggcggtg aagacgccat ggcggccggg cgtgaagccg ggggtcgcaa tgcgggcgat
     1021 cggtcccgac gggtccagca gcagcatgtt gccgaagagc gttgtggtgg tcggatcggc
     1081 cgcatcgacg cagcggtggt agtaggcgta ggtggcgccg ggggtcaggc cggtcaggct
     1141 cagccggcag tagaacggga tccggttgtt gttgacgccg ctcgcgccct ggacgaactg
     1201 ggggagctgt tcatcgcgga tcgactgggc ccaggtccgc gtgatcagcc ccggcaggcc
     1261 gaaaagcaag acgacgagca gcagccggca caaagcgggc gccttccatc aggagaaatg
     1321 gcgaatgtag agatattaag attccattag tcgaatctca aggcgaatcc gggcggggtg
     1381 acgggcaacg ggcgaggcgt cgcgctatgc gtcctcgggc gggatggctt ccagtttgcg
     1441 gccgcggcgg atcaggtgcc agcgttcgat cgtgcggagg cgggcgtcgc ggcggccgcg
     1501 ctcggcgaag gtcagcgcct cttcgacggc ggccagaccg ggcgggtggg ggccgcggcg
     1561 cagatcccag agccatgcgc gcaggagctg gcggcgcaac aggcggcccg atttgcgcca
     1621 cgccggccgg cccaggtcga gcgccgcgac cgccggcggc tcggcctggc agcgcagcag
     1681 ctccagcagg cgggcgaccg cgtcatcgag ggcgcgcgct tcctcctcga tcagttcggc
     1741 cgcgcggacg atccgttcgg tcggatcgat gcccagcctg tcgcgcagca gtgggatcac
     1801 cgcgtggcgc agccagccgc gacgcgtctc ttcgctggcg ttggtggggt cgtcgcgcca
     1861 ggtcaggccg cgcgccgtca ggaaccggcg caggtcggcc cggcgcagcg ccagcagcgg
     1921 gcgcacgacg gtgaggccct cgaccgcatc gatcggccgg atgccggcga ggccggtggg
     1981 gccgctgccg gccagcaggc gcatgaggaa agtctcggcg cggtcgtcgg cctggtgcgc
     2041 cagcacgagc gcgtcggcgc gccaggcctc ggcggcgcgg acgtagcagc gcaggcgggc
     2101 agcgcgcagg acgtcctcgc tgagcggccc ggccgcgacc agggactcgg gcaagcgttc
     2161 gatgaagcag gtcaggccga gcgcggcggc gcggtcgtgg acaaatcgag cctcgtcggc
     2221 gctttcgggg cgctgcgcgt ggtcgacgtg aacgacggcg agccgccagt cgcggcgggg
     2281 ggcctcggcg gcagccagct cgagcagcga catcgaatcg ccccccgccg agcagccgag
     2341 cagcaagccg gcgccggcgg ccagcggggc gagccggtcg caggccgaac ggaaaccgtc
     2401 ggtcacgcgc gcggcggttt cctcttcggg cgaagagctc acgggcggag cgggggtcgt
     2461 gcgtttgcgg gccacggagg caactcaagg gggccggccg ggcgagttgg aacggtccaa
     2521 ctatctcgcg gcggaaaggg aatggaaacc ggaaaagggc ggagcggcgg ggcgtcgcgg
     2581 ggccggtgca aacgccgttg cggcggaaga tgagttcatg gtagataatc ccgcccccgc
     2641 gctccggcgt ggggggttgc gcctgccgcc gcatggcgcg tcattccgaa cgccccgcgc
     2701 ccggagggaa gggcgccccc cgccgtgccc gccaacccgt gcctgcaatg cggcgcctgt
     2761 tgcgcgtttt accgcgcctc gttctattgg gccgaggccg acgacgccac gcccggcggg
     2821 gtgccggccg agctcaccac ggcgcatccg ccgcatttcc gcgtgatgcg cggcacggag
     2881 gggcccaacc cgcgctgcgt cgccctggcc ggtacgatcg gcgaggcggt ccactgcacg
     2941 attcatccgc gccgcgcgtc ggtctgccgc gacttcgtgc cttcctgggc cgagggggag
     3001 cgcaatgagc ggtgcgaccg ggcgcgcgcc gcgcacggcc tgccgccgct ccggcccgag
     3061 gactggattt ccccggacga cgacctggag ccggcggcgt agaaacggcg ccggacgtgg
     3121 acgcggcgtc acggcttaac ccgcgatctc gaccagttcg atgtcgaacg tcaggtcctg
     3181 gccggccagc gggtgattgg cgtcgacggt gaccagggtc tcctcgatct ccacgaccgt
     3241 caggacgagg gggtcctggc catcgcgcga cgccttgagc tgcatcccca gttccagcgg
     3301 gatgctctcg gggatcgcgc tgcggtcgat ctgctgcacc atctcctcgt ggtaggggcc
     3361 gtaggcctcc tcgtgaggga tccggacgat ctgatggtcg cccggcgtca tcccgacgac
     3421 ggcctgctcg aagccgggga tgatctggcc gtcgccgagg cggaactcca gcggttcgcg
     3481 cccgcgcgag gtatcgaaga cctggccgtc atcgagcttg cccgtataat ggactcggac
     3541 gagatcgccc agcttggctt gcgacatggg atcaattcct tcttgggagt tacaggaggg
     3601 ttgcagccga ccattcaggt ccagggaggg gtttgaagcg ggcggctgtc gcggaatgga
     3661 cgtctgtatt gaaccacaca gcggcgcggg gccgcaagcc gttagggacc catcggcggc
     3721 gtcctgcatc tcgcgaagat gcccgagggg aagaggctgg cctcggcggg cctgggtgag
     3781 aggggaaact attgaaaagg tttagaaaaa tctgtcgcgt cgggtccccg gacccccgag
     3841 ggggcccgga aaaagcccgc agacgcgggc tttcaaggcg cccgctatcc ggggattaag
     3901 aaggactttt cagagtcctc cggttcgcgg gctattgagt tgctcatcaa cctggggttc
     3961 acaaggcgga caaaatgtcg aggtcttgag agccgaggac gagggacgag agccgagagc
     4021 cgagagccga attttgggcg ggcctttgga agcgaggacg aggacgaggg acgagtttgg
     4081 tgagtttggc ggacgttccg cgcgcggagg cggcggtctc agcaaccgca tgtggacagc
     4141 tagcgcatcg gacgttccgc gcgcggaggc ggcgggaaca aattcgctgg caaaatggtc
     4201 cccttcgctg ttaagaaggg aaggtgaggc ggggcatccg aatcggacaa tacgttcacg
     4261 gcagggtggt ggattgagtt tgcgggactt aacgcagcag gggggagttc tgggcggcac
     4321 gaccgtcctg ctggcggcgc tgctcggcgt cggcctgtgg atgctgatgg accttcccga
     4381 cagcgaggcg cagccggcga ccagttcgcc cggcacgctg ccggaggata tctttgatct
     4441 cgactcggag cagatcattc tggaggattt ccagaccgag atcaccgagc aggacgaaac
     4501 gcagcatcgc atcagcgcca gctgggcgcg gctgaatgaa tccgatgaga tcatcgacat
     4561 gaagggcctg agcgtcgagt tctactcgga caaccggccg cgcggcgagg cgacgagcgg
     4621 ctatgggcgg atctggctgg aggacagcga ggaagacggc cggtcgcgaa atgatcttct
     4681 gctgagcgag acggtacgct actatacgat caaacaagag gacgacgagg aggcggccca
     4741 gcagggcgaa tgggtcctgg agacgccgct gatgctgttc accagcgacg attcgaccct
     4801 gcgttcggac aagggttacg tcaagcagat gccgatggaa tcgggggcgc tcggttcagt
     4861 cttctccggc gagtatttcg accgcgtccg ggccgaccgg attcagtacg acgcgcaggg
     4921 gtcggtggcg atgcggggca atgtgcgcta cgagagccag gcgctcggga tcgtcggtca
     4981 atcgctggcc gtcgacaccg caagcaaggt gatccagggc gcgggcgacc cgctgtggct
     5041 gcatcggggc gtcgatatgc aggcgcaatg cggcaagttc gtgtatgata cggaggccgg
     5101 ccgcatccag ttgacggagg agccggtggt ctaccagctt gccgagggca aggtgcagcg
     5161 gatccgcgcc ggcgtcgtca acatgatgga agacgaatcg ggccggctcg gcgtgagcct
     5221 gcgcgggggg cccggcgggc ggtttgagat cgatcggatc gacgaagtgc cggactcccc
     5281 gatcgtcctg cccgggagcg cgcccaagcc ggccgaagcc gcggccgatt ccgcagggca
     5341 gtttctggtg gggcgcggcg aacggtttga gatcaaagtc aatctggcgg ccaatacgtt
     5401 cgatacctgg acggaataca ccgcgattct cgaaaagacg gagcaaccag tcatactgcc
     5461 atgaaacgct gcctgctaat tatcccgaca tgcctcctgc tcgccgccgt ggtcgttccg
     5521 gcggtgggcg cgcaggaaga agaggggtcc gccggcctcg gctcgttcgc cggcatggac
     5581 tttgacaaga tccgggccca ccaactggac ctgtcgcccg acggcaggat caatctggtc
     5641 ggcgaggtcg atatcgagag cgagacgctc gcgatcaagg ccgacgtcgt cacgagcgac
     5701 gagacgggcg acgtgctgaa ggccaagggg agtcccgtac gcatccgcca gggtgatacc
     5761 gtgcgtgccg aatgccgcga cttcacgtat tacgtcgagt cgggcaagtc gatcctggag
     5821 ggctcgccgg tcatctatca gacgaccgag gaagggacga cgaaggtcgt cggcggcatc
     5881 atcatcctga cgaaggatga ggagggcaac acggggatca gcgtcaagat ggacccgggc
     5941 tccaagacga tgccgacgat cgagaacatc aagagcgccc cgaaggcgtc ggcgccgcag
     6001 gaaaagaagc ccgccacgaa ggtcaacgac tcgccggggc tgatccggct gccggcgctg
     6061 gaatagcccg gcaggcggcg gctcgcggcc gacacggctt tgggctggac atcgcgcgcg
     6121 gattcaccca atttaggatc gggtggcgcc cgcgtgtcgg actggacgcg aacgtccggc
     6181 cgggtccgtc ccgagcttca catcccacgg catgatctgg cacggcccgc cccggccgca
     6241 tccagacggc tcttgccccc atcccctgtt gtcagagtag gcctgcatga agatccttgt
     6301 aacgggcgcg gcgggattca tcggatcgca ccttgcggag cgactcgccg ccgccggtca
     6361 tgaagtcgtc ggcatcgact gtttcaccga gtattactcc accgccctga agcgcctcaa
     6421 cgccgaggac gtccggtctc acggcgtcac gattcacgag ctggacctgg ccgccgacga
     6481 cctgagccgc gccgttcgcg gcgtggaggc cgtcattcac ctggccgccc agcccggcat
     6541 ctcggaccac gtatcgttcc agacctacgt ccgcaacaac atggaggcca cctggcgcct
     6601 gctcgaagcg gtcaagagcc aggattcgtt gcgcggcttc atcaacgtcg ccacgtcgtc
     6661 ggtctatggc gcgcatgcca ccgacgacga ggaaacggcc cccaagccga cctcgtatta
     6721 cggcgtgacg aagctggcgg ccgaacagct cgtgctggcc tatcatcgcg ataaggcgct
     6781 gccggcctgt tcgctgcgga ttttctcggt ctacggggag cgcgagcgcc ccgagaagct
     6841 ctatcccaaa ctgatcagct cgatcctgga ggggcgtgag ttcccgctgc atcgcggcag
     6901 cgaccggcat tcgcggacgt tcacctacat cagcgacgtg ctcgacgcgt tcgaggcggt
     6961 gctcgcgcgg ctcgatgcgt gcaacgggga aatcctcaac atcggttcgg acatcgaaat
     7021 cacgaccggc gaggggatcg cgctgatcga gaagatcatg gggcgccggg cccggctggc
     7081 gatcaagccg ccgcgcgccg gcgatcagct caagacgcgc gccaacatcg aaaaggcgcg
     7141 gcggctgctg ggctgggagc cgcgtgttcc ccccgaggaa ggcctgcgcc gcgaagtgga
     7201 atggtatgaa aaccggatct acggccgggt cgcaatctag cggcgcgttg agacggtcat
     7261 gccgcgggct actccggcca ttccttgcag gggttcgagc cgatgcgcga gggtttggcc
     7321 ttcggctcct ggtcgcgcag catttccgct acctgcccgt ctggcgcaac aactggaacc
     7381 tgcacagcta tatggtcgag cgggtgccca acagcgcgcg ggcgcaactg ggctacgcgc
     7441 ggcagctagc ggaacgcgac gacctggagg gggcgatcgc gcacctccgg cagtcgctcc
     7501 ggatctatcc gacccgcatc gaggcggttg cgcggctcgg actgctgctg gccgaggtcg
     7561 gccgcgagga cgaggcgctc gatccgctct accgcgcgca tctggcggac ccgggccgtc
     7621 tggaactgac cgacgttcta gccgggattc tgacgcggcg gggcgagttc gagcgcgccg
     7681 aagcggtgct gcgccgctcg ctcgaagccc gtccgacctt cctgcggatt cagtacgact
     7741 acggccgctg gttggccgaa cgcggccgct accgcgaggc ccggccgctg ctcgagcagg
     7801 cgctgccccg gctgcccgac gatcatcgcg cgcgggttct gctgccgctg gccgagggct
     7861 accttgccgc gggcgacgcc gcgcgggccg aggcgatggt ccgtgcctgg ctggaagtcc
     7921 ggccggcgga ccccgacggc gcggcggccg cgctgttgga gcggatcgcc gccgcccgac
     7981 aaccggaagg gtgatctttc ggctaccgaa caagccggtt cgacgctccg gtgatggttg
     8041 accggcgggc gtgtgcggcg ctaacatccc gcgcatgaag gcgctggagg cccatcccgt
     8101 ggcggcggcc ggtgcgctga cggtcgccct tgccgccgtc ttcgaactca ccgaaatctc
     8161 ggtggtgttc ctgctcgggc tgatgggaat catcctgttc gtggggctgg tctggggccc
     8221 gcggctgccg ctgtttgtcc gttcatggtt cacctggatg atcttttatt ttggcgcgtt
     8281 ggcgtgcatg ttcggccgga cgatggcgcc ggggctgacg ctttacaccg cgggcatggc
     8341 tctgctgagc ttctcgtcta tggccttatt gccggcactg gcgctgcttc ggctgtggca
     8401 cggacgccgg cgcctcgcgc tgatcggcct ggtcctgccg gtttcgctgt tgctgggctg
     8461 cgtggttggg ttcaccgagg agcgtctctt catctggcgt taccgcgaaa cgggagcggg
     8521 gcccacgccg cgctggaccg tcgagatgca ttggctcgcc tatgacccgg cgaccgggga
     8581 actggccgga tcagattgag actctcccag cgtctccggc aacccctgga agggattgag
     8641 gcgtcgggcc ggcgacgccg ggttccggca tccggcctcc accccgcgcg ctagaccttg
     8701 tcattcccca aattcagatc ggctccggcc gcccgttcga ggggcttgga tcgctggagg
     8761 cggccaggcc gggttccggc ctgcggccgt caccctgcac tttactccgg tcgtccgctt
     8821 cgcggactca gccggcaacc gggtcggaat cgcccgcatt ggatttggaa tccctgagcg
     8881 gatcgcgacc tggggaatga caagcgcttc agaccggttg tccgctgcgc ggttggatcc
     8941 ggctaacatt tcggaagggc cgtgcttccc ccgtcccgcc gcctcctttc atcccggact
     9001 tcctgcttgc caagctcgcg gcgcgtgcca caatgcggcc gtgaacgacc gactccgggg
     9061 gagggaagag aaccatgacc gcgacgatga acacctggtg gcttttcatc ggccatgaag
     9121 agatcaagat tgaactgaag cagctcgagg acgaagggcg cgacccggcg ccggtgaaag
     9181 ccggactgga gcgtctgctc aagctgagcg gcgacgagct gcgcctgccg aagaaccagg
     9241 agaaggccgg cgagctgttc gaccgcgcgc ggacgctcaa gatgcgcaag ggctatgagt
     9301 ttgacgagcc gtcggatctg aagtcgatcc gcaagctgcg gccgcgcggt ccgcgcaaat
     9361 acgcccggcg gctgcccgac aaggtgctgc tcgaccggat cacggcggcg tggacggcgc
     9421 gctgctgcgg ctgcctgctg ggccgcgcgc cgatcgaggg tgcccgctcg tgggaaatgg
     9481 aaggcttcct caagatgacc ggccagtggc cgctgcgcga ctacttccgc ttcggcgtca
     9541 ggggcaaggc ggcccgggag tatccgaagt tcacggcggg ggcgcacctc gacacgatcg
     9601 accacatgcc ggtggacgac gacacgaact acacgacgac cgggttcctg ctggtgaagc
     9661 agcggggggc ggatttcgcg ccgcgggacg cggccgattt ctggatgatg aacattccgc
     9721 tgctggcgac gtgcaccgcc gagcgggtcg cctaccgcaa tttcgcgatg aatctggcgc
     9781 cgcccgacag cgcgacgttc cgcaatccgt ttcgcgaatg gatcggggcg cagatccgcg
     9841 ccgacgcgtt cggctatctg aacgccggcg atccacagcg cgccgccgag ttcggctggc
     9901 gcgacgcctc gatcagccat gtcaagaacg gcatctacgg cgagatgtgg gcggccgcga
     9961 tgatcgccgc cgcgccgtat tgcgagacgt tcgaggagat catccgcgtc gggctgagcg
    10021 agatcccccg gacgagccgc ctgcacgccg ccgtgagcga ggtgctggac tggcgcaagc
    10081 gggggctgac ctacgaggcg gcggtccaga aaatccacga ccgctgggac gagcagttcc
    10141 accatgactg gtgccacacg atcaccaacg ccgtcatcgt cgccgtcggg ctgttgtggg
    10201 gcgaggacga tttcggcacg tcgatctgcc gcgccgtgac gccgggctac gacaccgact
    10261 gcaatgccgc gacaatcggt tcggtcttcg gcatgcgtta cgggaccgaa gctctgcccg
    10321 agaagtggac aaggaagatc aacaacacgc tcaagacgag cctggtcggc tacgagacgg
    10381 tgcggctgga ccagatcgcg cgggaaacgt gggagctggc ccggaagatc cggtagcggg
    10441 gccgcggcgg caggagcgcc gcatctgggt ttggggaact ccgtcgactc ggtcggagcg
    10501 gtccgatggt ggctcgatcc gatgagtggg tggtctcggc ttgcgcgggg gaataaggga
    10561 tgttagggtc tagaacgctc gcgcgggtcg ggcggcgggt tttcgagagc cgaggacgag
    10621 ggacgagagc cgagagccga agttctcggc gggccgttgg gggccgatca cgaggacgag
    10681 gggcgagaga cgaattctag gaggaagccg ccgccatgga cgagaagaag atcatcctcc
    10741 ccgagcgtga gctgccgacg cactggtata acctggcggc ggatctgccc gagccgctcc
    10801 agccgccgcg ccatccggcc acgcatgcgc cgctgacgcc cgcggaactg gcgccgctgt
    10861 tcgccgaggc gctgatcgaa caggaactga gcaccgagcg ctggatcgaa atcccgacgg
    10921 ccgtgcggga ggcctaccgc gtctggcggc cgtcgccgct gtttcgcgcc accggcctgg
    10981 aggctgcgct ggagactccg gccgagatct ggtgcaaatg ggaaggggtc agcccggccg
    11041 gcagccacaa gcccaacagc gcgatcccgc aggcctatta caacgcccgc gaaggaatcc
    11101 gccggctgac gaccgagacc ggcgccggcc agtggggcag ctcgctggcc tacgcctgcg
    11161 cgcagttcgg gctggagtgc accgtctaca tggtgcggat cagcttcagc cagaaaccgt
    11221 atcgcgcgtc gctgatgcgg gcgtgggggg cgacggtctt tcccagtccg agcgaccaga
    11281 ccgggagcgg ccgcgccgcg ctggccgaag accccgagac caacggcagc ctggggatcg
    11341 cgatcagcga ggcggtggag gacgccgtcg cccacgacga cgcgcactac gcgctgggca
    11401 gcgtgctcaa ccatgtgctg ctgcaccaga cgatcatcgg gctggagacg cagaagcagt
    11461 tcaagcgcgc ggggcgcgag cccgacgtgc tggtcggctg cgtcggcggc ggctccaatt
    11521 tcgccggctt cgtcttccct tacattcccg agaagctcgg cgggcacaac ctgcggctgg
    11581 tcgcggtcga gccggcagcc tgcccgacgc tgacccgcgg ccggctggcg tatgactacg
    11641 gcgacaccgc cggcttgacg ccgctgatcc ggatgcacac gctcggcagt catttcatgc
    11701 cgcccgggtt ccacgccggc gggctgcgct accacggcat ggcgccgatg gtctcggccg
    11761 ccgtcgagca ggggctggtc gaaccggtcg cgatccccca gctcgagaca ttcgaggcgg
    11821 ggctcctctt cgcccggacc gaggggatca tgccggcgcc ggaggcgtgc cacgcgatcc
    11881 gcgccgcgat catcgaggcc gaacgctgca aggccgaacg gaagaaggag atcatcgccg
    11941 tcaacgtctc gggccacggc cacatggaca tggccgcgtt cgacgcctat ctgagcggca
    12001 agctggtcaa ccaggagcac gacgaacagg cgctggcgct ctcgctgagt cacctgcccg
    12061 cgatctgaaa ccgggatcgg gatcgatcga taccgattgc gaaagcgatc ccgatccgat
    12121 aaggatcggg agggatcagg ctgagatggc cgagttctcg gcgacgacgg gcggggcggg
    12181 cagggcttcg aagtaatcgg cggtggcggc gagcgtccgc tcgtcgagcc actcctcatc
    12241 cgtttcgcgc agggccagga tgatctgatc gcccgaccgg cgcaggcggg agaagacctg
    12301 ccggccggcg gcggcattcg cggccagcgc cgccgcgagt tcggcggcga agaccggcgc
    12361 cttgatccgg acggtgtcgt cggtgtaagg ctggatccgg tcgacgcgcc agggggcggc
    12421 ggccagcttc agccggcagg cgttgatcag gttgatcgcc ggttcgggca gcgcaccgta
    12481 acgatcgacg agttcctcga gcagcaggtc cagttcgtcc ttctcgcgcg cggcggcgat
    12541 gtccttgtaa aaccggatgc gctgcgattc gatcgggata tactcctcgg ggaggaactg
    12601 ttcgacgggc cacttgacgt cgaccggcca gagcggttcg cgcgcgccga cgccgcgcaa
    12661 ctcgctgacc gcctcctcca gcaactggca atacatctcg aagccgacgt cggtgatcgc
    12721 gccgtgttgt tcgcggccga ggatattgcc ggtgccgcgg atctccatgt cgcgcatggc
    12781 gatggagaag ccgacgccca ggtcggtgaa ctcctcgagc gcctcgaggc gcttgatcgc
    12841 ctgcggcgtg atcgcttcgc ccggcggcag gatcagatag gcgtaggcct ggcggacgtc
    12901 gcggccgacg cgcccgcgaa gctggtagag ctgcgccagg ccgaacgtgt cggcgcgatt
    12961 gatgatgatc gtgttgacgt tggggatgtc gatgccgttc tcgatgatcg tggtcgagac
    13021 caggatgtca tacttgccct cgatgaattc gagcatgatc cgctccaggt cctgctcgtc
    13081 catctggccg tgggcgacga cgatccgcgc gcgcgggacg atttcgcgga tcgtctgggc
    13141 gacgtcctgg atcgtctcga tccggttgtg gacgaagaag acctggccgc cgcgattcag
    13201 ttcgcgcagg atcgcctcct cgatctgctc gcggtcgaag cggatcgtcc gcgtcttgat
    13261 cgggtggcga ttggccgggg gggtcgtgat gatgctcagg tcgcgcaggc ccgacagcgc
    13321 catgtagagc gtccgcggga tcggcgtggc cgtcagcgtc aggatgtcga cgttggcccg
    13381 caggttcttg attttttcct tctgcgccac gccgaagcgc tgttcctcgt ccacgatcag
    13441 caggcccagg tcgcggaact gcacgtcgcg cgagagcagc cggtgcgtcc cgatgaccag
    13501 gtcgatctcg ccctccttca gttggcgcag gatctcgttg atctgcgcgg tggagcggaa
    13561 gcgcgagatc atctcgacct tgaacgggta atcggccagc cgctcggaaa acgtattgta
    13621 atgctgctgg acaaggagcg tcgtcggggc gagcagcgcg acctggcgct tctcgaccat
    13681 cgccttgaac gccgcccgga tggcgacttc cgtcttgccg tagccgacgt cgccgcagac
    13741 cagccggtcc attgggcgcg gctcgcacat gttggccttg gtctggcgga tcgcctcgag
    13801 ctgatcgggg gtctcctgat agatgaacga cgcctcgaac tcctgctgcc aggccgaatc
    13861 gggggcgtag gcgtagccct cggcggcctc gcggcgggcg tagagttcga gcagttcgcc
    13921 ggccatcttg cgcaccgctt ccatcgattt gcgccggcgg ctctgccatt tgcggctgcc
    13981 gagccggtcc agcgtcggcg gtttgccgtc ggccgaggca tacttctgaa tcaggtgcag
    14041 cttgtcgacc ggaacgagca gccggtcgtc gttctggtag accagttcga ggaattcggt
    14101 cagccggccg tcgacattct ggcggcggat gcgctcgaaa tgggcgatgc cgtgctcgat
    14161 gtggacgacg taatcgccgc gctggatttc ggtgggatta ctgatcgcct tgccgcgggc
    14221 ggccgccttg cgatagacgt ggcgacgctt gtagcggccg aagatctcgc ggtcggtgac
    14281 gatgaagatc ccggcctggg gcgagtggaa gccctcgtgc agatcgccca ccatcagcag
    14341 cacgtcgggg cagcggtcgg cggccgagcg cggcaacgcc ggggccggct ggccgtcgac
    14401 gagcggcagc gcgctgatct cgttctcgcg cagcagctcg tccagccgca tcacctggcc
    14461 gttgttgtcg cagacgatgg cgacccagtg gcccttgcgc aggcgctcgc ggatcaggcc
    14521 aaggtaattc tggaattgcg aggtgaccga ttcgaaaccc tgggcgggga aggtcaggcg
    14581 gcgaccggcg agggcctcct cggccggccg gttgtcctcc tcctcctcga tgtcggagcc
    14641 gagatcggtc aggtcgagcg attgaaaact cgtcagcgct tcctggaccg ccgtccggga
    14701 aaggagccac tggccggggg tcagattgtg ctcgacgaag aagctgacct ggtcgttgtg
    14761 ctggatgcgc tggatatcgc cccagtgacg ggtggcgatc tcgtcgaagc ggcgcaggcg
    14821 ctggtcgagc cggaacggcc cgtcgagcac gaccagcgag ttttcgggga ggaggtcgaa
    14881 cagcgagccg agcgcgtggc cggcgcgcag ccaggcgagc ttgggttcga cgcaaccggg
    14941 ggcgaggatc cgcaccgcct cggtttccgc gtcgcgcggc agcgagcgct gcgtctgcgg
    15001 atcgaacgcg cggatctgat cgatttcctc gccgaacagg tcgagccgcc acgggttgga
    15061 ggcgtcgggg ggatagacgt cgatgatcga gccgcggatc gcgaactcgc cggggctctc
    15121 gacgagcgcc tcgcggcgga agcccatgcc gaccagttgg gcggcgagcg cctcggggtc
    15181 aagcgtctgg ccccagcgga tcgtcagcga ctggcgcagc agcgcttcgc gcggcagcgg
    15241 ccggaaggcc agcgcgtcga ctggggcgac gatcagcggg gcggcctgcg gcgcctcggg
    15301 gtcgaggtgc gccagctcgg ccaggacggc gttgcgcgcg gcggcgatgt ggaggaccgg
    15361 ctcggtctct tcgaagggga gggtttcgaa gctggggtag atcagcggcc gggcgccgaa
    15421 gaacgccagg ccatcggtga gggcctcggc gcgttcgagc gagctggtga cgacgagcat
    15481 cgtgcggccg aggcggcggg caaggagggc gaggagagcg ggggcggcgc cggcgcgcgc
    15541 gccggtaaca accagtttat catcggcgcg cgcggcgcac tccacgatct ccgccgtcgc
    15601 cggttcgacc gccagccgct ccagaaagtg ggacagcacg cgctcctgca tacgcccccc
    15661 cagtctacta cgcccccgat ccgaaaaaca atgggcgcaa aacgggtg
//
LOCUS       DAOPCO010000067        15557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3864_length_15557_cov_5.608115, whole genome shotgun sequence.
ACCESSION   DAOPCO010000067 DAOPCO010000000
VERSION     DAOPCO010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15557
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_15557_cov_5.608115"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PLS90_06775"
     CDS             <1..273
                     /locus_tag="PLS90_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPK02145.1"
                     /translation="GRMLLVNRSNVTGLVDRMEEAGWVQRTAESGDRRVKLIGLTARG
                     RKLVERAEEAYFQSIDRLMDGIPRAELERMESILELLRSRTQQRQA"
     gene            275..2149
                     /locus_tag="PLS90_06780"
     CDS             275..2149
                     /locus_tag="PLS90_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02146.1"
                     /translation="MRPALAGAGSPTPRQPALALAGLLALAWAVRWLLAFCVPSPVIL
                     TDELQYIEMARHFPARGGLLWNGVQAWYPCWAYFVLIAPLARLPMPAALLAIRAINAA
                     LGSLGGLLAYGLAREFLTGRRALAAAALTLLLPAGAYTASVMTESLWVPVFVGAAWLG
                     ARAIAQPTRRRCLAAGAVAGLAFHVKPQGILIPAILALTAVIHEIRRLPSIAAESPDR
                     DGRQWRAWIRALAPHGVTALGWTLVAGLRVLEVVFVERPAHPFTPAAWLANYGRVADG
                     LYPLVPWQWPLSAAGYVLGWLLALGVLPAWLALRHPPPAAAPARGGDLGRILRVYTLV
                     GAALFIVVIARHTLVNDEGWRFHERYLVGLEPLVLILFCGALGPAADGRRWAAMFRID
                     LLFAVWLVCLFAGGRMAFTLPVDAPALTAVAALHQLRLLPIWAAVVIVLLFLGAPALV
                     IFSSPRPAARLGALGLLFAAMNFGYYASAHYITRWATEEERELAIGLERFVEPGDTLT
                     ILLDGLRPLQVHHAAFRRPAAVVFLSQSVREQANGWWARPMGLEQGRFRPAAGAGRHW
                     LLAGAAWRIHGARPVQLGTARLYPVPRDGLRFDPAQLADHLGGAAPVLPPRKVYPSPP
                     "
     gene            2519..2959
                     /locus_tag="PLS90_06785"
     CDS             2519..2959
                     /locus_tag="PLS90_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPK02147.1"
                     /translation="MKTLTPPRATVRPTRAPAAPRRKLFYRINEVSRITGIKPYVLRY
                     WETEFRELAPAKDSSDQRRYRQADIEVIQTIRRLLYEERYTIEGARKRLRVELRRQRE
                     DAQTAPAAASRPAQPAEPSPRDPRLTTALRNLRSEVNELLAILN"
     gene            3304..3861
                     /gene="pyrR"
                     /locus_tag="PLS90_06790"
     CDS             3304..3861
                     /gene="pyrR"
                     /locus_tag="PLS90_06790"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPK02148.1"
                     /translation="MMSRQLLDADQLQAAIVKLADGARRLAGSPHGAALVGIRTRGAV
                     LAQRLHGLLAADPAWKLPLGILDITLYRDDLSMLAPNPIVRKTEIDFDLTDKTILLID
                     DVLYTGRTIRSAIDAIMDYGRPRAIKLLVLVDRGGRELPIQADHAVLQVAVAEHEVVK
                     VCLAETDARDEVRLAPRQAQAVGGG"
     gene            3858..4487
                     /gene="tmk"
                     /locus_tag="PLS90_06795"
     CDS             3858..4487
                     /gene="tmk"
                     /locus_tag="PLS90_06795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPK02149.1"
                     /translation="MTQPPSPSAPSDSARPGRLIVIEGIDGTGKTTLARLLADALRES
                     GRDVVLSREPTDGPHGRRLRESARQGRHPRAEEEAALFLADRRDHVERLILPALRAGR
                     WVVLDRYFYSTMAYQGARGLDPAEIERANRAFAPSPDLLVLLELAVEDALERIRSQRG
                     TAPDHFEGRDYLEQVAKHFAAVEHPRLLRLDARLAPDQLLAEVMRALRD"
     gene            complement(4498..5562)
                     /locus_tag="PLS90_06800"
     CDS             complement(4498..5562)
                     /locus_tag="PLS90_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK02150.1"
                     /translation="MKTITNHERPLADRPPAEAAWQRFFDNPCEATFRALYEQTHALV
                     WTLCRRILKNPDDTQDAFQAVYCRLLKMAREGRGDELSGAPGEGVYRLAVRESNNLRM
                     QRRRRGEKEILMEQIPVVAAAERSAADLAQERQLREQVEALISTLPETLRVPLLLHYF
                     DGLSHRQIAAALDLPASTVSSRLAKGQRKLEPKLRKAGLGAVLMVLGTIAARAAPSLP
                     PAELTADAVYWRIEAMLQLQGGTAAGGAGAGSAGAAADVHWAAQFLTSRLGLLGLGIL
                     LLGGLTFLINRTHAWLPGGDDPAEKPAVTAEGPAETAHAVPCIMMQMAMQQAQRAAMN
                     QSAGQPVADAPSPPPAVAAN"
     gene            5956..6891
                     /locus_tag="PLS90_06805"
     CDS             5956..6891
                     /locus_tag="PLS90_06805"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPK02151.1"
                     /translation="MRFVDLGDPPPLKPTEILIATRYTGITNGTERHALLAEHGWTHF
                     PGRHGYQNVGQVAAVGREVKVFKKGDWVYCGLYIGHRGWIVADVGDAPAHAPGASLIL
                     RLPDAMDHSLAALFGVAGVALRGVRRARVAAAQNVWVAGQGLIGLFCGQCAHALGARV
                     TVADVRPDRLAIAAAAGAHRTIDMREAAAWDELQAAGPYDTIVEACGAERIFLDIHEH
                     ALLAPGGVILAIAVRSETTFHWATFHQREASIEVSCHFTLDDLRVLLHLVGEGRIQIA
                     PVVSHRVPITEAPRIYETLRDRPGELLGVIFDWSE"
     gene            6893..7963
                     /locus_tag="PLS90_06810"
     CDS             6893..7963
                     /locus_tag="PLS90_06810"
                     /inference="COORDINATES: protein motif:HMM:NF018178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPK02152.1"
                     /translation="MHAAREVRRVPRLALLVLTWALSLHGARAQDGPAELLPAWPLPA
                     EAAAILGDYAGTFSRTGLAPVPARGMVIARDGDEYRVPLWTVDPPATESLPVLVELTG
                     WRDDDQVFLAGEALGVKWQGLVHAGRLTAAIRHPYGGRFELRRADRKSPTLGAPSPAG
                     ATILLPYQPGQPTSLAAWEGAAWPLTEDGAVQVGRGDLRTVQMFGSVRLHLEFALPLI
                     ADVRGQKRANSGVFLHDRYEAQILDSWGVLAGAGDLGAIYNVSAPRVNAALPPLTWQT
                     FDMDFEAPTLTADGDAAAPAFMTVRLNGVLVQDRVALDHPTDASDKPPVARDCIRLQD
                     HGNPVRFRNIWLVETDGEKGAE"
     gene            7960..9093
                     /locus_tag="PLS90_06815"
     CDS             7960..9093
                     /locus_tag="PLS90_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02153.1"
                     /translation="MSWRAIHDGLAEMHPDLHWTPPREPADEPVIDHLGDPDAEARAL
                     HEQAALVVSPEWAPLHIEGRDAADYLHRRLSHSIRELTPGAGRYALQLAGDGHFQAEL
                     LVYRADEGFVALIDHAAREQVFALIEQYTLMDDVSVERMWPAEAVVGLAGPAAPGILA
                     ALLDPPATAPLLAPERFTGLMTVTLRGLPCRIFTDPRWPFPFYHLSIPGMAAARLARL
                     LAEMCAAAGGRPSGFAALQLARVRAGIPRQGWDLDPGVIPLEARLEAAIDRDKGCFPG
                     QEVIARIANLGHPARLLLRLRVDADQPVSAGDAIIIDEEIAGRITSAAGWPGLPYRLA
                     LGYVKWPHRAAPRALVQLADGTRLAASCSIASPTRSGEDEARG"
     gene            9143..9469
                     /locus_tag="PLS90_06820"
     CDS             9143..9469
                     /locus_tag="PLS90_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02154.1"
                     /translation="MSDRKPFELDDTGHSLRPIGEDSGKIRPVGGGGPAEPAAPATAP
                     APPGAAAPAPSRAGAPARTERTRVPARRDSLIVLALLLLAAIGAWLMYGPYIRMLFAR
                     LFGAAE"
     gene            9716..15490
                     /locus_tag="PLS90_06825"
     CDS             9716..15490
                     /locus_tag="PLS90_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPK02155.1"
                     /translation="MRLRLLAAILVSLLPLLHAASLTAALFTVTTTADENDGTPDPGV
                     EGTSLREAILAANANAEADEIRFDAALAGDELSVSLFDTGRSDGEFGPTAFSVFTEIE
                     INGPAGDDGITIKRAGGDNFRLFHVTITGDLTLRNLTLQGGVAQGFDGGSGGGGGGGS
                     AGVGGAVLNQGTLTLDGSTLTGNMALGGHGANSGNANDRGGGGGAGVGQHGADGASET
                     FGVTAPGGAGGDPNGGAPAGSGGGGDGGSGITAAASVVGGPGADGGFFFGGGGGGGSA
                     RSASGNSQTGGAGGAGGYGGGDGSAGGAGTNSPNGDGGGGAGLGGAIFNHSGTVVIRN
                     STLSGNVARGGDCGNPAFISLGDAGGGSALGAAIFSRNGSLYLINATIARNELVAGRS
                     LTSGADGDADGAVFGLGDGATFRLVANNSIVADTTGGDDVLVRIVNGGTSLIGGVNNL
                     VESFIGLGPSQIATNDDPALADLADNRGPTPTHALLPGSPAIDAGNNDGASGLLTDQR
                     GGIFDRFADGDLDGDARVDIGAFELTPIDYGDAPDFFPATGPASILLEPPKHDDFRIS
                     FMGDDAEMSPVVRTLYAGRDSHAAYNSVEDEFLVVWVGDTDAGNLSDNEFEIYAQRIS
                     GDGAKLLDDPIRVSVMGRDGDFDFQAADPAVVWNARDNLYLVVWWGDDDADPLVDNEF
                     EIFGQFLTGKGELMGDRIRISFMGPKGSTAYRAQHPDVAYNPTDNEYLVVWSGEHDKS
                     PLVDNEFEIFAQRISANGLLEDSMIRVSFMGDDAQSDPGQRAAFGAINPRIAWNSEMH
                     QYLAVWSADDDTPPLVDNEAEIFGRILDRQANPTGNQQRLSFMGPDGNSDFRAGGPDV
                     AAAGDEFFVVWYGDDNTGALVDNENEVYGRRVSAADAAPLGDQLRISQAGGTGQTFFS
                     AIDPRVGFNPESKNYFVVWRANSLMVGKYDIFGRRVSIGGTVIDNDDLRVSAMGPADD
                     PDFTAFSPALAANSKSGSYLVTWNGDDDIPPLIDGETEIFGQFLTETPYVDYNTRADD
                     FGPAHRLTRALMIGADIDSELNGQPSLQALGDDLEGESPNDEDGVDASLMIQASPAPT
                     VAVPVVNALAGDATLNAWIDYNGDGVFSNATERVSTTVPAGGPTTVTLTLPPVPPIHA
                     SPTFARFRLSTDPAAADPTGFALDGEVEDHALEIVPLPLFPEIRVAGNGLEIADGQTT
                     TSLENHTDFGDALVDSETVVRTFTIANLSTETALDLTGAFPVVLSGPGDAHFSVAAQP
                     ATDPIPPGEATTFQLTFDPATTGTLTATVTIGNDDPDEAPYSFAIRGEGLLVRPIYPE
                     IAIHGGQDLEIADGTTTTSIQNGTDFGPVHHPLGRTDRSFVIHNISAEAELHLTTMPA
                     VLITGPGADHFSIHKDPETNALPPLGTTYFIIRFDPSTTGVMDAIVEIPNNDPDENPY
                     RFAIQGEGILATEFPDIAVFGNGLEIADGSTTTSAINGTDFGTVLTSADPVGHSFDVR
                     NTGEAELVFEGELRPELSGPDADQFSLQYVLDIHSLQPGESSFFMVFFGPTSPGVKTA
                     TVTIASLVLDENPYTFVVRGVARHPAEIAILGNGVEIANGDDTPSLADHTDFGVLDFP
                     STAVARTFTLDNTAGLGDLDLLTTPPVQLSGPAADQFLVTLQPAPNPVPAGSSSPFII
                     LYQPTRPGTHRATVIIDNNDPDESTTTFAVQGIRPLPAGNGVGAVINYLLGISSDAMG
                     LDFNEDATVDIADAIDRVNAVPPAAPALPNPADAAGDVPTTASLAWQGDARALDYDLW
                     LWPAAEARPSTPSATGLIDPAFTPSAPLAPATLYNWQVRANGYGAQTEGPVWGFTTQP
                     AAKHAAGPPAHAPAWLKQRVNDDHFTLSAAPSPWERRRADFLKCCGRAWQRSTPPPPW
                     IARARPIPTCFQMISLKRLRPHPA"
BASE COUNT         2282 a         5958 c         4753 g         2564 t
ORIGIN      
        1 gggcgcatgc tgctggtcaa ccgctccaac gtgaccggcc tggttgatcg catggaggag
       61 gccggctggg tccagcgcac cgccgaatcg ggcgaccgcc gggtcaagct gatcgggttg
      121 accgcgcgcg gccgcaagct ggtcgagcgc gccgaggaag cctacttcca gtcgatcgac
      181 cgcctgatgg atgggatccc gcgcgccgaa ctggagcgga tggaatcgat cctcgaactg
      241 ctccgatcgc gcacccagca acgccaggcg tgacgtgaga cccgcgcttg ccggtgcagg
      301 ctcaccgacg ccgcgccagc ccgccctggc gctggccggg ctgctggcgc tcgcctgggc
      361 ggtccgctgg cttctcgcgt tctgcgtccc ttcccccgtc atcctcaccg atgaactgca
      421 atatatcgaa atggcgcgcc acttccccgc ccgcggcggc ctgctgtgga acggcgtgca
      481 ggcgtggtat ccctgctggg cctacttcgt cctcatcgcg ccgctcgccc ggctgccgat
      541 gcccgccgca ctgctggcga ttcgcgcgat caacgccgcg ctcggctccc tcggcggcct
      601 gctggcttac ggactggcac gcgagtttct gaccgggcgc cgggcgctgg ccgccgccgc
      661 gctcaccctg ctgctgcccg cgggcgccta caccgcttcg gtcatgaccg agtcgctctg
      721 ggtccccgtc ttcgtcggcg ccgcctggct cggcgcccgg gcgatcgcgc aaccgacccg
      781 gcggcgctgc ctggccgccg gagccgtcgc cggcctggcg tttcacgtca aaccccaggg
      841 gatcctgatc cccgcaatcc tcgccctcac agccgtcatt catgagatcc gccggctgcc
      901 gtcgatcgcc gccgaatcgc cggaccggga tgggcgccaa tggcgcgcct ggatccgcgc
      961 gctggcgccc cacggcgtca cggcgctggg ttggacgctg gtcgccggtc tgcgcgtcct
     1021 cgaggtggtt ttcgtcgagc gcccggccca tccgttcacc cccgccgcat ggctcgccaa
     1081 ttacggccgc gtcgccgacg gcctctatcc gctggtcccc tggcagtggc ccctctcggc
     1141 ggcgggctat gtgctcggct ggttgctggc gttgggcgtg ctgccggcgt ggctcgccct
     1201 gcgccatccc ccgccggcgg cggcgccggc gcgcggcggc gacctggggc ggatcctgcg
     1261 ggtctacacg ctcgtcggcg cggccctgtt catcgtcgtg attgcgcgcc acacgctcgt
     1321 caatgacgag ggctggcggt ttcacgagcg ctatctggtc ggcctcgagc cgctggtcct
     1381 gatcctgttt tgcggcgcgc tcggaccggc ggccgacggc cggcgctggg cggccatgtt
     1441 ccggatcgat ctcctcttcg ccgtctggct cgtctgtctg ttcgcgggcg ggcggatggc
     1501 ttttacgctg cccgtcgatg cgccggcact gacggccgtc gccgcgctgc accagctccg
     1561 gctcctcccg atctgggcgg cggtcgtcat cgtgctgctg ttcctcggcg cgccggcgct
     1621 ggttatattc agttcccccc ggccggcggc acgcctcggc gcgctcgggc tgctgtttgc
     1681 cgcgatgaac ttcggctatt acgcgagcgc gcactacata acccgctggg ccaccgaaga
     1741 ggaacgcgaa ctggccatcg gcctggaacg ctttgtcgag ccgggcgaca cactgaccat
     1801 tttgctcgac gggctccgcc cgctgcaggt gcaccacgcc gcgttccgtc ggccggccgc
     1861 ggtcgtattc ctgtcgcagt cggtccgcga gcaggccaac ggctggtggg cgcggccgat
     1921 gggactggag cagggtcgtt tccgcccggc ggccggcgcg gggcgccatt ggctgctggc
     1981 gggcgcggcc tggcgcatcc acggggcgcg gcccgtccag ctcggcacgg cgcgcctgta
     2041 tccggtcccg cgcgacggcc tccgcttcga ccccgcccaa ctcgccgatc atctcggcgg
     2101 cgccgcgccc gtcctccccc cgcgcaaggt ctatccgtcg cccccctgat ctcccgactc
     2161 gccatcggta tcggtatcgc cagcggaatc gtcatcgagc gcgccccctc gcttttctca
     2221 ccgcgcgccg cttttccatt gcatctcgcc cgcccgcgct ttatggttgc ttccggttga
     2281 tttacctgag cttcattcaa gccggaaagg aggatcgccc cccgacacgc atgacacacc
     2341 cttgcccggt tccgtcgtcg cggactgacc cgggccgatc gcatcgacca accgttgttt
     2401 cgttccgctt gccggcgttg aacggttccc gttgatccct tggttgctcc acccggcgca
     2461 acctcctctt gttcccgttg ctgaacctat ttcccctcgc aggaggtgtc gattcgcgat
     2521 gaaaacgctg accccacccc gcgccaccgt ccgcccgacg cgcgcacccg ccgccccccg
     2581 ccgcaaattg ttctatcgca tcaacgaagt ctcccgcatc accggcatca agccgtacgt
     2641 cctgcgctac tgggagaccg aattccgtga actggcgccc gccaaggatt cctccgacca
     2701 gcgccgctac cgccaggccg acatcgaagt catccagacc atccgccggc tgctctacga
     2761 ggaacgctac acgatcgagg gcgcgcgcaa acgcctgcgc gtcgaactgc gtcgccagcg
     2821 cgaggacgcg cagaccgccc cggccgccgc atcgcgtcct gcccagcccg ccgaaccgtc
     2881 ccctcgcgac ccgcggctga ctaccgccct gcgcaacctg cgcagcgaag tcaacgaact
     2941 gctggccatc ttgaactgaa cctcgcccgc cccgagtccc cccggcccac ccccgggggg
     3001 accgcccccc atcgatttcc gtccctcgtc tctcgtcctc gtgatccccc ccaacggcct
     3061 acccaagaat tcggttctcg gctctcgtcc ctcatcctcg gccctcgaaa acccgccacc
     3121 ttccctcctc attccaaacc gcccgcatcc ctgcctgcag ccatttccaa cttgcagtgg
     3181 gcggttttcc atttgccaac gccccgccga agcgccagaa tttcgtcttt tcccggcgcg
     3241 ccgggatccc cacggagacc ggccgccgcg tttccgtcca cgagccgatt ccgaacaggc
     3301 acgatgatga gccgtcaact gctggacgcc gatcaactcc aggccgcgat tgtcaaactg
     3361 gccgatgggg cgcgccgcct cgccgggtcc cctcacggcg ccgcgctggt tggtatccgc
     3421 acgcgcggcg ccgtcctcgc ccagcgcctg cacggcctgc tcgccgccga ccccgcctgg
     3481 aaactcccgc ttggcattct cgatatcacg ctctatcgcg acgatctgtc gatgctcgcc
     3541 cccaacccga tcgtccggaa aacggaaatc gacttcgatc tgaccgacaa gaccatcctg
     3601 ctgatcgacg acgtgctcta caccggccgg acgattcgct ccgccatcga tgcgatcatg
     3661 gattacggtc gcccgcgcgc gatcaagttg ctcgtgctgg tcgatcgcgg cggccgcgag
     3721 ctgccgatcc aggccgatca cgccgtgctc caggtcgccg tcgccgaaca cgaggtcgtc
     3781 aaggtctgcc tggccgagac cgatgcccgc gatgaagtgc ggctcgcgcc ccgccaggct
     3841 caggcagtcg gcggcggatg acccagcccc cttcacccag cgctccctcc gattctgccc
     3901 gacccggacg cctgatcgtc atcgaaggga tcgacgggac gggcaagacc acgctggccc
     3961 ggctcctggc cgacgccttg cgcgagagcg gacgcgacgt ggtgctctcg cgtgagccga
     4021 ccgacggccc ccatggtcgc cggctgcgcg aatccgcccg ccaggggcgt catccccgcg
     4081 ccgaggagga ggcggcgctc ttcctggccg atcgccgcga ccatgtcgag cggctgatcc
     4141 tccccgccct gcgcgcggga cgctgggtcg tccttgatcg ctatttctat tcgacgatgg
     4201 cctatcaggg ggcgcgcgga ctcgatccgg ccgagatcga acgggccaac cgcgcctttg
     4261 cgccgtcgcc cgatctgctc gtgctgctgg agctggcggt cgaggacgcc ctggaacgca
     4321 ttcgctccca gcgcgggacg gcccccgatc attttgaagg gcgtgactac ctggaacagg
     4381 tggcgaagca cttcgcggcc gttgagcatc cacggctgct gcgcctggat gcccggctcg
     4441 cgcccgatca attgctggct gaagtcatgc gcgccctgcg cgactgatcg cgccggttca
     4501 gttggccgcg acggctggcg gcggcgacgg cgcgtccgcc accggctggc ccgccgactg
     4561 gttcatcgcc gcccgctgcg cctgctgcat cgccatctgc atcatgatgc acggcacggc
     4621 gtgcgcggtc tcggccggac cttcggccgt gacagccggc ttctccgccg gatcatcgcc
     4681 tcccggcagc caggcatgcg tccggttgat caggaacgtc agccctccca gcagcaggat
     4741 ccccagcccc agcaacccga gccgcgacgt gaggaactgc gcggcccagt ggacatcggc
     4801 ggcggcgccg gccgatccgg cccccgcgcc ccccgcggcc gttcccccct gaagctggag
     4861 catcgcttcg atccgccagt acacggcatc ggccgtcagt tcggccggcg gcaggctcgg
     4921 cgcggcgcgc gcggcgatcg tccccagcac catcagcacc gcccccagcc ccgccttgcg
     4981 cagtttcggt tccagcttgc gctgaccctt ggccagccgg ctcgacaccg tgctggcggg
     5041 caggtccagc gccgcggcga tctggcggtg gctcagcccg tcgaagtaat gcagcagcag
     5101 cgggacccgc agcgtctcgg gcaacgttga aatgagcgcc tcgacctgtt cgcgcagttg
     5161 ccgttcctgc gccaggtcgg cggccgagcg ttccgcggcg gcaacaacgg ggatctgctc
     5221 catcaggatc tccttctccc cccggcgccg gcgttgcatc cgtaaattat tggattcgcg
     5281 caccgccagt cgatagacgc cctcgcccgg cgcgcccgac agctcgtcgc cgcggccttc
     5341 gcgcgccatc ttcaacagac ggcagtagac cgcctgaaac gcatcctgcg tgtcatcggg
     5401 attcttcagg atgcgccggc aaagggtcca gaccagcgcg tgcgtctgtt catacaatgc
     5461 gcggaacgtc gcttcacacg gattgtcgaa aaagcgctgc caggccgcct cggccggggg
     5521 gcggtccgcc aacgggcgct cgtgattcgt gatcgttttc atccccatcg ctccacaact
     5581 tccgtctcat cttccaaatg cacgccccgt gggcgggctg tacgacgctt tttctgcttt
     5641 cgtcccggct ccccgcgtcc ggccctcggc ggccgattga tgatggaagg gtaatctatc
     5701 gctcccttca ggctactccc cgtcccccga cctgtcattc gaaaatgcgg ccccaccccc
     5761 cgcacgattc aattgtctgt tcagcccgcc tgccgcagaa tcaggagccg gcgccgcccg
     5821 ccgcccgggc gctgggacat catcccaaac agtccttacc ccccgcagag gtcgaatcct
     5881 cgcccgccca aaccattctg gaactgagtc aatcccatgc gcagaatcgc catcgaatac
     5941 ccggatcgga gccaaatgcg gtttgtcgac ctcggcgatc ccccgccgct taaaccgacc
     6001 gagatcctga tcgccacccg ctacacgggc atcaccaacg gcaccgagcg ccatgccctg
     6061 ctggccgaac acggctggac gcatttcccc gggcgccacg gctatcagaa cgtcggccag
     6121 gtcgccgccg tcggccgcga ggtcaaagtc ttcaagaaag gcgactgggt ctactgcggg
     6181 ctttacatcg gccaccgcgg ctggatcgtc gccgatgtcg gcgacgcgcc ggcccatgcg
     6241 cccggggcct cgctgattct gcgcctgccc gacgccatgg atcattccct cgcggcgctg
     6301 ttcggcgtgg ccggcgtggc gctgcgcggc gtccgccgcg cccgcgtcgc cgccgcccag
     6361 aacgtctggg tcgccggtca gggtctcatc ggcctgttct gcggtcagtg cgcccatgcg
     6421 ctgggcgctc gcgtcacggt cgccgacgtc cgcccggacc ggctggccat cgccgccgcc
     6481 gccggcgccc atcggacgat cgacatgcgc gaggccgcgg cctgggacga gctgcaggcc
     6541 gccggcccct acgacacgat tgttgaggcc tgcggcgccg aacggatctt cctcgacatc
     6601 cacgaacacg ccctgctggc ccccggcggc gtgatcctgg ccatcgccgt ccgcagcgaa
     6661 accactttcc attgggccac gttccaccag cgtgaagcct cgatcgaggt ctcctgccat
     6721 ttcaccctcg acgacctgcg cgtcctcctg catctggtcg gcgaggggcg gatccagatc
     6781 gcgccggtcg tcagccaccg ggtgccgatc accgaggcgc cgcggattta cgaaacgctc
     6841 cgcgatcggc ccggtgagtt gctcggcgtc atctttgact ggagcgagtg agatgcacgc
     6901 cgcgcgcgag gtccggcgag tcccccggct cgccctgctc gtcctgacct gggccttgtc
     6961 gctccatggc gcccgggccc aggacggccc ggcggagctc ttgccggcct ggccgctccc
     7021 ggccgaggcg gccgcgatcc tgggcgacta tgccggcacg ttctcgcgca cgggcctcgc
     7081 gcccgtcccc gctcgtggga tggttatcgc ccgcgacggc gacgaatacc gcgttccgct
     7141 ctggacagtc gacccgccgg cgaccgaatc gctcccggtg ctggtcgagt tgaccggctg
     7201 gcgcgacgac gaccaggtgt tcctcgccgg cgaagcgctc ggcgtgaaat ggcaaggcct
     7261 ggtgcacgcg ggccggctca ccgccgccat ccgccaccct tacggcggcc ggttcgagct
     7321 gcggcgcgcc gatcggaaat cgccgaccct cggcgcgccg tcccccgccg gcgccaccat
     7381 cctcttgccg tatcaacccg gccagccgac gtcgctcgcc gcctgggagg gcgccgcctg
     7441 gcccctgacc gaggacggcg cggttcaggt cggccgcggc gatctccgga cggtccagat
     7501 gttcggctcc gtgcgcctgc atctggaatt cgccctgccg ctgattgccg acgtccgcgg
     7561 tcagaaacgg gccaacagcg gcgtctttct ccatgaccgc tatgaggcgc agatcctcga
     7621 ctcctggggc gtgctggccg gcgcggggga tctcggtgcg atctacaacg tctcggcgcc
     7681 gcgggtcaat gccgccttgc cgccgctcac ctggcagaca ttcgacatgg atttcgaggc
     7741 gccgacgttg accgccgacg gcgatgcggc tgcgcccgcg ttcatgaccg tccgcctcaa
     7801 cggcgtcctg gttcaggatc gtgtggcgct cgaccacccg accgatgcca gcgacaagcc
     7861 gcccgttgcg cgcgactgca tccgcctgca ggatcacggc aaccccgtcc gcttccgcaa
     7921 catctggctc gttgaaaccg acggagaaaa gggagccgaa tgagctggcg cgcgattcac
     7981 gacgggttgg ccgagatgca tcccgacctc cactggacgc ctccccgcga gccggcggac
     8041 gaaccggtga tcgaccacct cggcgatccc gacgcggagg cccgcgccct ccacgagcag
     8101 gcggcactgg tcgtctcccc ggagtgggcg ccgctgcaca tcgagggccg cgacgcagcc
     8161 gattatctcc accgccggct gtcgcactcg atccgcgagc tgacgcccgg cgccggccgc
     8221 tacgccctgc aactggccgg cgacggccat ttccaggccg aactgcttgt ctaccgggcg
     8281 gatgagggat tcgtcgcgct gatcgatcac gccgcccgcg aacaggtctt cgcgctgatc
     8341 gagcaataca cgttaatgga cgacgtgagc gtggagcgga tgtggcccgc cgaggcggtc
     8401 gtcggcctgg ccgggcccgc cgcgccgggc atcctcgccg cgctgctcga tccccccgcc
     8461 acggcccccc tgctcgcgcc ggagcgcttc accggcctga tgaccgtcac gctgcgcggt
     8521 ttgccctgcc gcatcttcac cgatccgcgc tggcccttcc cgttctatca cctttcgatt
     8581 cccggcatgg cggcggcgcg cctggcgcgc ctgctggccg agatgtgcgc ggcggcgggg
     8641 ggccggccgt ccggtttcgc cgcgctccag ctggcccgcg tccgcgccgg catcccgcgc
     8701 cagggatggg acctcgatcc gggtgtcatc ccgctggagg cccgcctgga ggccgccatc
     8761 gaccgcgaca agggctgctt ccccggccag gaggtcatcg cccgcatcgc caaccttggc
     8821 catccggcac gcctcctgct ccgtctgcgc gtcgacgccg accagcccgt ttcggcgggc
     8881 gacgcgatca ttatcgacga ggaaatcgcc ggccgcatca cgtcggcggc gggttggccc
     8941 gggctgccgt atcgcctcgc cctgggttac gtgaagtggc cccatcgcgc cgcgccgcgc
     9001 gcgctggttc agctcgccga cggaacgcgg ctggcggcgt cgtgttccat tgcatcgccg
     9061 acccgttcgg gcgaagatga ggcgcgcggg taagccgtaa caccgatcag gtccgcggcg
     9121 gagcgggccg gagcgccgac gcatgagcga caggaaaccc tttgagctgg acgacaccgg
     9181 tcactcgctg cggccgatcg gcgaggattc cggcaagatt cgcccggtcg ggggcggcgg
     9241 ccccgccgaa ccggccgcgc ccgcgaccgc gcctgctcca cccggcgcgg cggcgccggc
     9301 cccatcgcgc gcgggcgccc cggcccgcac ggaacgcacc cgcgtccccg cccggcgcga
     9361 ctcgctcatc gtcctcgcgc tcctcctgct ggcggccatc ggcgcctggc tgatgtacgg
     9421 cccgtacatc cgcatgctct tcgcccgcct cttcggcgcc gccgagtgat ggtccagaat
     9481 catccctcgt cctcgtcctc ggctctcgaa aaccctccat tctcgccacc ccccggttaa
     9541 cgttttccct gcgttttccc atcctgccgg ggctatactg actcaagaca tttcgtgctt
     9601 tgctctcgaa cggccacagg tcgccgaacc gttggccatg ccttctcgcg gttcgcttca
     9661 ttaaccggct ggacgaacca cgttgtgtcg agttctcccc gcaaaggaga cccccatgcg
     9721 cctccgcctt ctcgccgcga tccttgtctc cctgctcccg ttgttgcacg cggcgagcct
     9781 gacggccgcc ctcttcaccg tcacgaccac cgccgacgag aacgatggga cgcctgatcc
     9841 cggggtagag ggaacctccc tgcgcgaggc gatcctcgcc gccaacgcca acgccgaggc
     9901 ggacgaaatt cgattcgacg ccgcgctcgc cggcgacgag ctgtccgtct ccctcttcga
     9961 taccggccgg agcgacggcg aatttggccc gacggccttc tccgtcttca ccgagatcga
    10021 gatcaacggg cccgccggcg acgacggtat cacgatcaaa cgggccggcg gtgacaattt
    10081 ccggcttttt cacgtgacca tcaccggcga cctcaccttg cggaacctga cgctacaggg
    10141 gggcgttgcc cagggttttg acggcggatc gggcggcggc ggcggcggcg ggtcggccgg
    10201 cgtcggcggc gcggtcctga atcagggcac gctcaccctc gacggatcaa ccctgacagg
    10261 gaacatggcc ctgggcggcc acggcgccaa ctccggcaat gccaatgatc gcggcggcgg
    10321 gggcggcgcc ggcgtcggcc agcacggcgc cgacggcgcc agcgagacat tcggcgtcac
    10381 cgcccccggc ggcgccggcg gcgatcccaa tggcggcgcg ccggccggca gcggcggcgg
    10441 cggcgacggc ggttcgggca tcaccgcggc cgccagcgtc gtgggcggcc ccggcgccga
    10501 tgggggcttc ttcttcggtg gcgggggcgg cggcggctcg gcccgctccg cctccggcaa
    10561 ctcgcagacc ggcggcgccg gcggcgccgg cggctatggc ggcggcgatg gctccgccgg
    10621 cggggccggc accaacagcc ccaacggcga cggcggcggc ggcgcgggcc tcggcggcgc
    10681 catctttaac cacagcggca ccgtcgtcat ccgcaacagc acgctttcgg gcaatgtcgc
    10741 gcgcggcggc gattgcggca accccgcctt catcagcctg ggcgacgccg gcggcgggtc
    10801 cgccctgggc gcggccatct tcagccgcaa cggcagtctc tacctcatta acgccacgat
    10861 tgcccgcaat gagcttgtcg ccggccggtc gctcacgtcc ggggccgatg gcgacgctga
    10921 cggcgccgtc ttcgggctcg gcgacggcgc cactttccgg ctggtcgcca acaactccat
    10981 tgtggcggat acaaccggcg gcgacgacgt cctcgtgcgc atcgtcaacg gcggcacgtc
    11041 gttgatcggc ggcgtgaaca acctcgtcga atccttcatc ggcctggggc cctcccagat
    11101 cgcgaccaac gacgatccgg cgctggccga cctcgccgac aaccgcggcc cgacccccac
    11161 gcacgccctc ctccccggca gccccgcgat cgacgccggc aacaacgacg gcgccagcgg
    11221 cctgctcacc gatcagcgcg gcggcatctt cgaccgattc gccgacggcg acctggacgg
    11281 cgacgcccgc gtcgatatcg gcgccttcga gctgaccccg atcgactacg gcgacgcgcc
    11341 cgatttcttc cccgcaacgg gtccggcgtc gattctgctc gaacctccca agcacgatga
    11401 tttccgcatc agcttcatgg gcgacgacgc cgaaatgtcg cccgtcgtcc gcacactcta
    11461 cgccggccgc gattcgcacg cggcctacaa ttccgtcgag gatgagttcc tcgtcgtctg
    11521 ggtcggcgat accgacgcgg gcaatctgag cgacaatgaa ttcgaaatct acgctcaacg
    11581 catcagcggc gatggcgcca agctgctcga tgaccccatc cgcgtcagcg tgatgggacg
    11641 cgacggcgat ttcgacttcc aggcagccga tcccgccgtc gtctggaacg cgcgcgacaa
    11701 tctgtatctg gtcgtctggt ggggcgacga cgacgccgac ccgctcgtcg ataatgaatt
    11761 cgaaattttc ggccagtttc tcaccggcaa gggcgagctc atgggcgacc gcatccgtat
    11821 cagcttcatg gggcccaagg gctccacggc gtatcgcgcc cagcaccccg acgtcgccta
    11881 caaccccacc gacaacgagt atctggtcgt gtggagcggc gaacacgaca agtccccgct
    11941 cgtcgacaat gaattcgaga tcttcgccca gcgcatcagc gccaacggcc tcctcgaaga
    12001 ttcgatgatc cgcgtcagct tcatgggcga cgacgcgcaa tccgatcccg ggcagcgcgc
    12061 ggccttcggc gccatcaacc cccgcatcgc ctggaattcg gaaatgcacc aatacctggc
    12121 cgtctggagt gccgacgacg acaccccccc gctcgtcgat aacgaggccg aaatcttcgg
    12181 gcggatcctc gatcgccagg ccaaccccac cggcaaccag cagcgcctca gcttcatggg
    12241 acccgacggc aattccgatt tccgcgccgg cgggcccgac gtggccgccg ccggcgatga
    12301 gttcttcgtc gtctggtatg gcgacgacaa caccggcgcc ctggtcgaca acgaaaacga
    12361 agtctatggc cgccgcgtca gcgccgccga tgccgcgccc ctcggcgacc agctccgcat
    12421 cagccaggcc ggcggcacgg gccagacctt cttttccgcc atcgatccgc gggtcggctt
    12481 caatcccgaa agtaagaact acttcgtcgt ctggcgcgcc aattcgctca tggtcggcaa
    12541 gtacgatatc tttggccgcc gcgtgagcat cggcggaacc gtcatcgaca acgacgatct
    12601 ccgcgtttcc gccatgggcc ccgccgatga ccccgacttc accgccttca gccccgcgct
    12661 cgccgccaat tccaaatccg gttcctacct ggtcacctgg aacggcgacg acgacatccc
    12721 cccgctgatc gatggcgaaa ccgaaatctt cggtcaattt ctcaccgaga ccccctacgt
    12781 cgattacaac acgcgggccg acgacttcgg tccggcccac cggcttaccc gcgcgctcat
    12841 gatcggcgcg gacatcgact cggagctcaa tgggcagccg tcgctgcagg ccctcggcga
    12901 cgacctggag ggcgaatccc ccaacgacga ggacggtgtt gacgcatcgc tgatgatcca
    12961 ggcttccccg gcgcccacgg tggccgtgcc cgtcgtcaat gctctcgcgg gcgacgccac
    13021 gctaaatgcc tggatcgatt acaacggcga cggcgtgttc agcaacgcga ccgagcgcgt
    13081 ctcaacgacc gttcccgccg gcgggccgac caccgttacg ctgacgctgc cgcccgtgcc
    13141 gccgatccac gcctcgccga cgttcgcccg cttccgcctc agcaccgacc ccgccgccgc
    13201 cgaccccacc ggcttcgccc tcgacggcga ggtcgaggat cacgcgctcg aaatcgtccc
    13261 gctgcccctc ttcccggaga tccgcgtcgc cggcaatggc ctcgagatcg ccgacggcca
    13321 gaccaccact tcgctggaga accacaccga cttcggcgac gccctggtcg attccgagac
    13381 ggtcgtgcgc acgttcacga tcgcgaacct gtcgaccgag accgcgctcg acctgaccgg
    13441 cgcgtttccg gtcgtcctga gcggccccgg cgacgcgcat ttcagcgtcg ccgcgcaacc
    13501 cgcgaccgat cccatccctc ccggggaggc gaccacattc cagcttacgt tcgatcccgc
    13561 cacgaccggc acgctgacgg cgaccgtgac gatcggaaat gatgaccccg atgaagcgcc
    13621 ctattcattc gcgatccggg gtgaagggct cctcgtccgg ccgatctatc cggagattgc
    13681 gatccatggg ggccaggatc ttgaaattgc cgacggcacg accacgactt ccatccagaa
    13741 cggcaccgat ttcggcccgg ttcaccatcc gttgggcagg accgatcgat ccttcgtcat
    13801 ccacaacata tcggcggaag ccgagctgca tctcaccaca atgcccgcgg tcctgattac
    13861 gggtcccggc gcggatcact tttccatcca caaggacccg gagacaaacg ccctgccccc
    13921 actgggcaca acctacttta ttattcgctt tgacccctcc acgacaggcg tcatggacgc
    13981 catcgtcgaa atcccgaata acgatccgga tgagaatcca taccgcttcg cgattcaagg
    14041 cgaggggatt ctcgcgacgg agttcccgga tatcgcggtt ttcggcaacg gtctcgagat
    14101 cgccgacggt tccaccacaa ccagcgcgat caacggcacc gatttcggaa ccgtcctgac
    14161 cagcgcggac ccggtgggcc attcattcga cgttcggaat accggcgagg cggaactggt
    14221 tttcgagggc gaattacggc ccgagctgag cggacccgat gccgatcaat tctctctcca
    14281 gtatgttctg gacatccatt cgctccaacc cggcgaatcc tcctttttca tggttttctt
    14341 tggtccgacc agccccggcg tgaaaaccgc gacggtcacg atcgcaagcc tcgtcctcga
    14401 tgagaatccc tatacgttcg tcgtccgcgg cgtggcgcgc catccggccg aaatcgcgat
    14461 cctcggcaac ggcgttgaga tcgccaacgg cgacgacacc cccagcctcg ccgatcacac
    14521 cgacttcggc gtcctcgatt tcccctccac tgccgtcgcg cgcaccttca ccctcgacaa
    14581 caccgccggc ctgggcgacc tcgacctgct caccacgccg ccggtccagc tctccggccc
    14641 cgccgccgat cagttcctgg tcacgctgca accggcgccc aatcccgtcc ccgccggtag
    14701 ttcttcgccg ttcatcatcc tgtaccagcc gacgcggccc ggcacgcacc gcgccaccgt
    14761 gatcatcgac aacaacgatc cggacgaatc gacgacgacc ttcgccgtgc agggcatccg
    14821 cccgctgccc gccggcaacg gggtcggcgc cgtcatcaac tacctgctgg gcatcagctc
    14881 cgatgcgatg gggctggact tcaacgaaga cgcgacggtc gacatcgccg acgccatcga
    14941 tcgcgtcaat gccgttccgc ccgccgcgcc ggcgctgccc aacccggccg acgccgccgg
    15001 cgacgtcccc accaccgcgt cgctcgcctg gcagggcgac gcccgcgcgc ttgattatga
    15061 cctgtggctc tggcccgccg ccgaggcgcg cccctccacc ccgtcggcca ccggcctgat
    15121 cgacccggcc tttacgcctt ccgcgccgct ggcgcccgcc acgctctaca actggcaggt
    15181 ccgcgccaac ggctacggcg cccagactga gggccccgtc tggggcttca ccacccagcc
    15241 agccgccaaa catgccgccg gcccgcccgc ccatgctccc gcgtggctga aacagcgggt
    15301 aaacgacgac cacttcacgc tctctgccgc cccatcccca tgggaacgac gtcgcgccga
    15361 cttcttgaag tgctgtggca gagcatggca gcgcagcacg ccgccaccgc cttggatcgc
    15421 ccgcgcccgg ccgatcccga cctgtttcca gatgatctcc ctcaaacgcc ttcggcccca
    15481 ccctgcctga gaatccccca cgcctctctc aatcctctta tgtcctttgc gtcccttctg
    15541 tcccttcctt tttgccc
//
LOCUS       DAOPCO010000068        15282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3935_length_15282_cov_4.334209, whole genome shotgun sequence.
ACCESSION   DAOPCO010000068 DAOPCO010000000
VERSION     DAOPCO010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3935_length_15282_cov_4.334209"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="PLS90_06830"
     CDS             <1..423
                     /locus_tag="PLS90_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK02156.1"
                     /translation="GELQIPVIEELTDTITEHPPTSEGGARAMRRILRRNQPFSAVFS
                     FDDFTAYGVIQELYSQGLRVPEDVSVVGFDDIWLSKAYNPPLTTIYHPMAELGVSGAK
                     LLIKKLKSEAPKNGAAQAQEIFLRPSLVMRSSTASHSQ"
     gene            703..1926
                     /locus_tag="PLS90_06835"
     CDS             703..1926
                     /locus_tag="PLS90_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02157.1"
                     /translation="MDVTLSMFGPTAHGESPEQTAAALRTAGANQLMFFTSLYHGYRL
                     LQPRYPDRAIYTLETDRVFYEPDPALYKECPVKPERSRDFPDRDWVTDFGKALHAEGI
                     QFSPLVPVCAAGRLVQERPDLAVRNLYGAADRLFLCYNNPDVRAYRLAMIRDIVGNHE
                     IDNLMLDKIPQTMLEQYAFSGIFDPPLRTVGSFCFCEHCKRRAERSDLDLGEVQAKAR
                     EIALRSLRIPQYVIAAQGEKLVGDTEIPLLLLEEPLIYRMLQFRFESAVELVGEIRDL
                     VKQLRPKARLQAAFVPPSHFGHDMTSPRAWLTIQSYQKYVDVLDEIYCVVHYGADVVR
                     FETERAVAAAKGKAKIVTSMRLYGATRPEEVALLSEAALAGGSQGVSFLGYDVTTDEL
                     LGELGQWVKGRGVHN"
     gene            1926..3257
                     /locus_tag="PLS90_06840"
     CDS             1926..3257
                     /locus_tag="PLS90_06840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK02158.1"
                     /translation="MSRLAIQGGEPIRNRPFAKWPEVDQHDEQNVLEVVRSGNWWMYS
                     YGKDEFAGTVKGTSRVEAFEKAFGKMLHARHTYATASGSACLEIACRAIDLQPGDEVI
                     TTPYTFIATSTCILNALAIPVYVDIEPETYNLNVDLVEAAITDRTRAIIPVHFGGVIA
                     DMDRLQSIARRHNLKIIEDAAHAHGASLKGDRWAGTLGDIGIFSLQQSKLLTCGEGGA
                     ITTNDPELAELAWSLRHYGRTQTGKWYEHFRLGWHYRMTEMQGALLLSQLDKLPRQNE
                     IRRRNARLLREELTAIPGIKVAPQHPETENDVYYVICLRYDAEQWDGIERDLVIKALE
                     AEGIPVFSGYTFPLYENPLFQNIDFNSERSPYRIGRDRPLADFREYRERCPVAERACR
                     RESMWLTLDMLMGDREDTRDIVRGFTKVYEHRHALRALANGSVAETLQTTR"
     gene            3294..4703
                     /locus_tag="PLS90_06845"
     CDS             3294..4703
                     /locus_tag="PLS90_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02159.1"
                     /translation="MKAGYASADITPDREVTLCGFAARCDQPATGIDDRLIVHALALQ
                     QRGETVLLLAFDLLGLGPEAVDRIHEGLDAAGVPAPREQRIFCCTHTHGAPAAVKLIG
                     CGVIELTYLEQLTTAATQAAQAAIQSLQPAVLRKALLEVPEGNYNRRRVLEDGRVVMA
                     QQPDAPVARTGPVWNELMLLRWEDQQGQPISGLAIWAAHACTVCDSRITGDFPAELCR
                     RLAERYGHPFMYLQGSCGNLNPPLLEMNRGEMLGNVTRLMNCLPRDNEAVPWSKPLPT
                     DPFGVTAEPIRLQYQALMGSDELQAIRDGMRSIEETGKGPIRFVAELTNILNTEPGED
                     PDPIMLRYIASILRRWGEETLQDGERRLREGCMLDIRTLHLGATALCFVAAEPFVETA
                     YALREACPDRNVSLIGYGSPLIGYLPTDPALDEGGYEVDYAYRFYGHPAGFAKGSEPA
                     ALRAMIHTVNAQAAVAGRA"
     gene            4708..5769
                     /locus_tag="PLS90_06850"
     CDS             4708..5769
                     /locus_tag="PLS90_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02160.1"
                     /translation="MVCIGIIGTGFMGRTHAAAYEKSSHVDQVRLLTTAQELEAVERL
                     AGDYSKVSGIDTDAELFFKRGIDAVDICTPTPTHAAFVRRAIAARKPILCEKPLSLDP
                     GQADALVREAEAANVPFMSAQVIRFWPEYVFLKRAIDEGTLGQPLVLSMSRFSPLPNW
                     GVNQWFGDLGLSGGALFDLHVHDIDFLQHALGLPRRLSAIGLKREGLAYTDISTTLDF
                     GDDIRATIYASYERPAGSAFHMGYRALFDGGAIDYDIERTPTLRQWTGGAEQTMDFSE
                     EPNGYERECDEFAGCVAQGKPPAVASAFSAIPTLRLLAAIARSADHAGQWIDVEQGDG
                     QRPFGEPVNTTAAGRESEA"
     gene            5766..6548
                     /locus_tag="PLS90_06855"
     CDS             5766..6548
                     /locus_tag="PLS90_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPK02161.1"
                     /translation="MSAKVAMAVSAHPDDIELMMAGTLILLGAQGYQMHMMNVADGSC
                     GTASLPVGQIIEQRRQEAQNAATTIGACYHPPLVHDFGIYYEPKLLAKLAAVVRRVKP
                     TILLLPAPEDYMEDHINASRLMVSAAFTRGMANFTTDPPTAPYDAPVALYHALPWGLL
                     DPLRRPVPAELFVDIGSVLDKKTSMLACHVSQKDWLAQSQGVDYLKLMREMAAQVGSH
                     ARPGSYAEGWRRHLHLGFAPQDYDPLSEALADFLIGTVHTPE"
     gene            6600..7577
                     /locus_tag="PLS90_06860"
     CDS             6600..7577
                     /locus_tag="PLS90_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HPK02162.1"
                     /translation="MPSQIIEQARSASRNLQDRPLSLVAPEWWDYTTLDAELLEEAAR
                     LDVADIAGLARDGFRIVFHDTLEEFYLAEALEYIDAWRQATPDSPAGICGPIGPTEQL
                     PLVAALVNELQLDLRHAFFWGMDEWVIDGRSVSRDHPLSFARADMELCFDRIRPELAM
                     PAENLHFPTEDPKDYLSSWDQARCVVMQGGQGEVKHWAFNDPLRREGPWKEAPPTAEE
                     YLRLSTRVVELHPLTLIQNARTSGGGNLTLVPTHAVSVGPRETWRAEKVSIWHAGTHD
                     NPFGMRLTTLMIAKQIVDTAVPMSLLGLHPNVQFNFYRPCLGTCAVEMH"
     gene            complement(7683..9692)
                     /locus_tag="PLS90_06865"
     CDS             complement(7683..9692)
                     /locus_tag="PLS90_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02163.1"
                     /translation="MFFMMHDARFNCPASLRFVIGLGLITVLPALLRAEETLPLIANG
                     EPSARIVLLDPPSVCLDLAAGVITKTVERWSGGTVLPRERPTEAAAAPAPAGETTILL
                     TTLTALRETYPELIQAHPALLRANFTDPHGFSLAAVDLPDGRRAMAVVSSTERGVLNG
                     AHYLRDFLIDGSAGDLSLKTPSVVRSPQMLVRGTYCLAIYGVMPQYTVEDWRKVFEGF
                     ARDGMERVYFWVSGMFPSEKFPQTFDRDASQGTVIRETGQIREIIQAAHDVGLKLYLG
                     SGVFAWSTVAYLGEGLEDAAAVGVGGLCPENAEIRQRHMEYFLEMADALPEADGFFFE
                     LRDEHGECQCDECQVPVDSHGSKMYGEYLISFVQQFTEQLWRKHPTFGLCVNVGYDEH
                     KDDPNFYAQITRMNDPRFEWLDCRWSWTFPTTLGERVPGVYISRRMIHWGPFYSRPLR
                     EMVEMTSKAAREGYIGYLPAYEPGYSTADFYSHEIPYPMDRLPYLLTGFVYRELTWEP
                     IFAGEELQQRIHDRFFCQEAPLELADDLQDLRDWVVANAAAINRFSQDWINYSAVRVP
                     YPNLGEEVAKVEQMEPVYYEGQATPLLKQVKTLLQIRDEGLKRLAEIEANVEANRSDA
                     TPKTLETLDQMQRLIDDSRASYDRAVPEPAALDDYLARLEALLQQ"
     gene            complement(9832..11160)
                     /locus_tag="PLS90_06870"
     CDS             complement(9832..11160)
                     /locus_tag="PLS90_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK02164.1"
                     /translation="MNPSETSAKSNATYTPAESNLAARRWFEDARFGLFIHWGVYSVP
                     GRGEWVMEVEGIPIVEYDKFAREFNPVRYDPAQWVALAKRAGMKYITITTRHHDSFSL
                     WDSQAGEFNVVRSTPYGKDLLKPLAEECRRQGLKLFFYYSHLDWRHPDYFPLGNTGHS
                     AGRAEGGQFDRYVDFMLAQLTELLGGNYGEVAGIWFDGWWDQQIKRIKHLDRPDAPPT
                     ETQIDWRLTETYEHIHRLQPAALIGNNHHLEPFPGEDFQMFEKDLPGANTTGFSEDAR
                     IGALPLETCETINNNWGYHAGDHAHKSTRELIHYLVRAAGMGSNFLLNIGPRPDGTIQ
                     PEFVERLEAIGDWLAAHGESIYGTRGGPIAPQPWGVTTQKDGRVYVHVLAPPESGSLT
                     LPGGARLGFARARLLDTGQPLELRTRDADGYLSLPTRSDDLADIVIVLEK"
     gene            complement(11202..13928)
                     /locus_tag="PLS90_06875"
     CDS             complement(11202..13928)
                     /locus_tag="PLS90_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02165.1"
                     /translation="MATVQHEISRSVGYRLMALLCGCALLTAMFAPRDARAEDSMEKG
                     YTYSFWPRNPQRVEQPTDYRYAVRTDQYGVLLNVHGARLERLGAIASSSAAAALHQTD
                     VLETLAPVKLDWWMEVAGRRYRIVSGAWNPLAERNVIWQGLRLIDGGRWLQHFEIINV
                     RFADEEGQPLEGLNASLRFACWPDCMVVTLHLNTTLDYDVVSGRRPLRIGIDLPVGQA
                     QDTRWVQDSHPAEWRPAEPGMSQVVTGWAATNDESGGLAALFAADGDWSIAWSEDGQP
                     CVARNLGAWAAFDPLSFTCVLVPRRSGVLAHSDRVRTEKGLGVRAQTAEGQPLPVSFS
                     KTLWAHVVELPAQDDLWSEERTALALNHDLATTQQGRIIFQKDYDFHTNTNDRTTKNL
                     IGFSAHLTDPETGGEPLGLPVQISKNWHVMRNWSRAIALLAAPPGDRSLELVRVNALW
                     AGVPAISHAQLCLIGWGGNQLWEQVALGSFGEAICYDPSVNLQRSVVDDIRPAFVWGM
                     SEEPQKWVWTTNVGGADFLVVETPESQADRRQFEQQMRELEAGDRRPGDRIYPTWRLL
                     PDGQKTLYHKAGPVLSEVSYGGSYADGRIRSDVTTYSWRSDDFFRAVFHIRYDCLEAV
                     EFERLAFFQLGADFYNDNLFTTMARGDEDGLTEQWSPPMGEAAGYSRRNEPVAGANPW
                     FALMGTQPEDDRFPHAAWANRMLVVREWRARLGGRDDVPIHYAVYGTSNRTESAVLEL
                     SPPASLERLEPGDYVETWLEMSVLPQKAQDYYGENRALREQLMNYPDSWPLAHWVARS
                     RPEIKVHEGHQQRSYLPLIRAEQDRAEWTMTAGHGWWPVTIDNLSRPGPYRLEVKGEG
                     DWQVLEASSERQFIRQTDGNPANATWSLSYSFELPEGESRRYRLAPEDAASE"
     gene            complement(13993..>15282)
                     /locus_tag="PLS90_06880"
     CDS             complement(13993..>15282)
                     /locus_tag="PLS90_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02166.1"
                     /translation="LWGVVYTDLFQFILATVGTIILAFLAVRAVGGLEMMVERLTALE
                     EWSGSELLIRPSVGAAGNQMSVWNAIGYFGILWISVALAGGYQAQRVLACRDSRHASL
                     AMLLHTMLYYAVICWPWIIVALCSILLIPNLGEGVSHDAAYPRMIVSLMPTGLRGVMI
                     VALLAAFMSTISTLFNWGSSYIVNDLYKRFLVREASSRHYVMVGRAATFFMAAMGAVI
                     SLYARDIQQLLTISYVVGSSITLVGLMRWFWWRLNAWGELAGAVSGWTLAGLLLFARI
                     FDAPARYLFGPESNLSSDSDLLGARMMTVMLVVAVVAVVVSLLTPPTEADRLEQFVRR
                     ARPFHFFWKPIIRRFTEPGYETESLSRTLVSWGAAVLAVYSLMFGIGKVLLGQAVVGG
                     AALLVFGIALWVTVRRISQDFEHELVPVPEAVSAPEG"
BASE COUNT         2983 a         4775 c         4585 g         2939 t
ORIGIN      
        1 ggcgaactgc agattcccgt catcgaggag ttgaccgata ccatcacgga gcatcccccg
       61 acatcggagg ggggcgcgcg cgccatgcgc cgcatcctac ggcgtaatca gcccttttcg
      121 gctgtcttct cgttcgacga ttttacggct tatggtgtga tccaggagct ttacagtcag
      181 ggactgcgcg taccggagga tgtctccgtt gttggatttg acgatatctg gctgtcgaag
      241 gcctacaatc cgcccctgac gacgatctat cacccgatgg ccgaactggg cgtcagcggc
      301 gccaaattgc tgatcaagaa gttgaaatcg gaagcaccca agaatggggc tgcacaggcc
      361 caggagattt tcctgcgtcc gagtctcgtc atgcgcagca gcacagcgtc ccactcccaa
      421 tgagctaaga cggcgccaac gttcccgttg ccggctgata cgctgccggt cagcgctcgg
      481 tgtttggttt ggctctgctg caccgcaaag tttcgcttga cacttcgggc acccaatcgt
      541 taaatgcatt taacatacgc tgctctgtct cctttgtcga tccgctcgct agcggttttg
      601 gacaagagcc cagcggagcc caaatgcctg tctgccgcca ccctgcggca cggcctgcgt
      661 tggtcagaat gcgatggccc aggttagaag gactgcgtcg tgatggatgt caccttgtca
      721 atgttcgggc cgaccgctca cggcgaaagc cccgagcaga cggccgctgc gttgcgcacc
      781 gcgggcgcca atcaactgat gttctttacc agcctctacc acggctaccg tttgttgcag
      841 ccacgttacc cggatcgggc catctacact ctggagacgg atcgagtgtt ttacgagccc
      901 gaccccgcgc tctacaagga atgcccggtc aaacctgagc gcagccgtga ctttcccgat
      961 cgcgactggg tcactgattt cggcaaggcg ctgcatgccg agggcatcca gttttcgccc
     1021 ctggtgccgg tctgtgccgc cggccggctg gtccaggagc gtcccgatct ggccgtgcgc
     1081 aatctgtatg gcgcggccga ccgccttttc ctgtgctaca acaatccgga cgtgcgggcg
     1141 tatcgcttgg cgatgatccg ggatatcgtc ggcaatcatg aaatagataa cttgatgctt
     1201 gacaaaatcc cgcagacgat gctggagcaa tatgcattct cggggatttt cgacccgccg
     1261 ctgcgcaccg tgggcagctt ctgcttctgc gagcactgca agcgacgcgc agagcgctcg
     1321 gatctcgatt tgggcgaggt ccaggccaag gcgcgggaga tcgcgctccg ttcgcttcgc
     1381 atcccccagt acgtcatcgc cgcccagggg gagaagctgg tgggtgatac ggaaatcccc
     1441 ctcttgctgc tggaggagcc gttgatctac cggatgctgc agttccgttt tgagtcggca
     1501 gtcgagttgg tgggcgaaat tcgcgacctc gtcaagcaac tccgtcccaa ggcccgcctg
     1561 caagcggcat ttgtgccccc ctcgcacttc ggtcatgata tgacctcacc gcgcgcgtgg
     1621 ctcacgattc agagttatca gaagtatgtc gatgtcctcg acgaaatcta ttgcgtggtt
     1681 cactacggcg cggatgtcgt tcgcttcgag acggagcgcg cggtcgcggc cgccaagggg
     1741 aaggccaaaa ttgtaacgag catgcgcctt tacggggcaa cccgtccgga agaggtcgct
     1801 ttgctgtccg aggccgccct ggctggcggt tcgcaagggg tctcctttct cggatacgat
     1861 gtcaccaccg atgaactgct gggtgaactg ggccagtggg tcaaaggccg gggagtgcac
     1921 aactgatgtc gagactggcc attcaaggtg gcgaacccat ccgcaatcgg ccgttcgcga
     1981 aatggcccga agtcgatcag cacgacgagc agaacgtgct ggaagtggtc cgcagcggca
     2041 attggtggat gtacagttac ggcaaggatg agttcgcggg cacggtcaaa ggcacttccc
     2101 gggtggaagc atttgaaaag gcattcggga agatgctgca tgcccgtcac acctacgcga
     2161 cggcgagcgg atcggcctgt ctggaaatcg cctgtcgggc aatcgacttg cagccgggcg
     2221 atgaggtcat caccacccct tataccttca tcgccaccag tacctgcatt ctgaacgcgc
     2281 tggccattcc ggtgtatgtg gatatcgaac cggagaccta caatctgaac gtggatctgg
     2341 tcgaggcggc gatcaccgac cgcacgcgcg ccatcattcc ggtgcacttc ggcggcgtga
     2401 ttgcggacat ggaccggttg cagtccatcg cccgccgcca caacctgaag attattgagg
     2461 acgcggcgca tgcgcatggc gcctccttga aaggcgaccg ctgggccggc acgctcggcg
     2521 acatcggcat tttcagcctc cagcagtcca agctgctgac ttgcggcgag gggggggcga
     2581 tcaccaccaa tgatccggag ttggccgagc tggcgtggtc gctaaggcac tacggccgca
     2641 cccagaccgg caagtggtat gaacattttc gtcttggttg gcactatcgt atgaccgaga
     2701 tgcagggggc gctgttgctg agtcaactgg acaagctgcc caggcagaat gaaattcgcc
     2761 ggcgcaatgc ccgtctgttg cgggaggaac tgacggcgat tcccggcatc aaggtcgcgc
     2821 cacagcaccc agaaaccgaa aacgatgtct attacgtcat ttgtctgcga tacgacgccg
     2881 agcaatggga cggcatcgag cgcgacctgg tgatcaaggc gctcgaagcc gaaggcatcc
     2941 cagtattcag cggctatacc ttcccgctct acgagaaccc cctgttccag aatatcgact
     3001 tcaacagtga acgatccccc tatcggatcg gccgcgatcg gcctctggcc gacttccggg
     3061 agtaccgcga gcgctgtccc gtcgccgagc gggcctgccg tagagaaagc atgtggctga
     3121 cactggatat gctgatgggc gatcgcgaag acacacgcga catcgtccgc ggattcacca
     3181 aggtgtatga gcaccgccac gcgttgcggg cgcttgccaa cgggtcggtg gctgagaccc
     3241 tgcaaacgac gcgctgatgc catggaagat aagcaaagag ggtttgccct gagatgaagg
     3301 ctggatacgc cagcgctgac attacgccgg accgggaggt gacgctctgc ggctttgccg
     3361 cgcgctgcga ccaaccggcc acgggaatcg acgaccggct catcgtgcat gcccttgcat
     3421 tgcaacagag gggcgagacc gttcttctgc tggcgtttga cctgttagga ctgggtcccg
     3481 aggccgttga ccggatccac gaaggactgg acgcggcggg cgttccggcc ccccgtgagc
     3541 agcggatctt ctgttgcacg cacacgcatg gcgcaccggc ggcggtcaag ctcatcggct
     3601 gcggtgtgat cgagctcacc tatctcgaac agctaacgac tgccgcaact caggcggccc
     3661 aggcggcaat ccagtcactc cagccggcgg tactgcgcaa ggcgctactc gaagtccccg
     3721 aaggcaacta caaccgccgt cgggtactgg aggacggccg ggtggtgatg gcgcaacagc
     3781 ccgatgcccc cgtggcgcgg accggtccgg tctggaacga attgatgctg ctgcgctggg
     3841 aggatcagca agggcagccg atttccggtc tcgccatctg ggccgcgcat gcatgcacgg
     3901 tttgcgacag tcggatcacg ggcgattttc cggccgagct ttgccgccgt ctcgccgagc
     3961 gctacgggca tccgtttatg tatctacagg ggtcttgtgg caatctgaat ccaccgctat
     4021 tggaaatgaa tcggggggaa atgctcggca acgtcacgcg tctcatgaat tgcctgcccc
     4081 gagacaacga agcggttccg tggagcaagc cgttgccgac cgatccattc ggcgttacgg
     4141 cggaaccgat tcgcctgcag taccaagcgc tcatgggaag cgatgaactc caggcgattc
     4201 gcgacggcat gcggagcatc gaagagaccg gcaaaggacc gatccgattc gttgccgaat
     4261 taaccaatat cctgaacacc gaaccgggtg aggatcccga tccgatcatg ctgcggtata
     4321 tcgcctccat tttgcgccgt tggggcgagg agacgctgca agatggcgag cgtcgacttc
     4381 gtgaaggctg tatgctggat attcggacgc tgcatctggg cgctacggcg ctctgcttcg
     4441 tcgccgccga accctttgtc gagacagcct acgcgttgcg cgaggcgtgc cccgatcgca
     4501 atgtgtccct gatcggctac ggcagcccac tgattggcta tctgcccacc gacccggcct
     4561 tggacgaggg gggctatgaa gtggactacg cctaccgctt ctatggccat cccgccggat
     4621 tcgccaaagg gtcggaaccg gccgccctgc gtgcgatgat ccatacggtg aatgcccagg
     4681 ccgccgtagc agggagggcg tgatatcgtg gtatgtattg gaatcatagg cacgggattc
     4741 atgggccgga cgcatgcggc ggcctacgaa aaatcgtccc atgtcgatca ggtgcgcctg
     4801 ctgacaacgg cccaggagtt ggaagccgtc gagcgtctgg ccggcgacta ttccaaagtc
     4861 agtggcatcg atacggacgc cgaattgttt ttcaagagag ggattgacgc ggtcgatatc
     4921 tgcacgccga cgcccaccca tgcggcgttt gtgcgccggg cgattgccgc gcgcaagccg
     4981 atcctctgcg aaaagccgct cagccttgac ccgggccagg ccgacgcgct ggtgcgtgag
     5041 gcggaagcgg cgaacgtgcc gttcatgtca gcccaagtga ttcgcttctg gcccgaatat
     5101 gtgttcctca agcgtgccat cgacgaaggc actttgggtc aaccgctcgt tctgtcaatg
     5161 tcgcgttttt ctccgctacc caactggggg gtcaatcaat ggtttgggga tctgggattg
     5221 tcgggtggag cactattcga tctgcatgtc catgacatcg actttctcca gcacgcgctc
     5281 gggctgccgc gccgcctctc ggcgatcgga ctcaaacgcg aagggctggc ctacaccgac
     5341 atttcaacga cgctggattt tggcgacgat atccgtgcca cgatctatgc cagttatgag
     5401 cggcccgcgg gttcagcgtt ccacatgggc tatcgcgcgc tctttgacgg aggcgccatc
     5461 gactacgaca tcgagcgcac gccaacgctc cgccaatgga cgggcggcgc cgaacagaca
     5521 atggattttt cggaggaacc caacggctac gaacgcgagt gtgatgagtt cgccggttgc
     5581 gtggcacaag ggaagccgcc ggcggtcgca tcggcgttct cggcgatacc gaccctgcgg
     5641 ttgttggcgg ccatcgcccg ttcggcggat catgccggcc agtggatcga tgtggagcag
     5701 ggagatggcc agcggccgtt tggggaaccc gtgaatacaa ccgcggcagg gagggaaagc
     5761 gaagcgtgag tgcgaaggtc gccatggccg tttcggccca tcccgatgac atcgaactga
     5821 tgatggccgg gacattgatc ctgctgggtg cgcagggtta tcagatgcac atgatgaatg
     5881 tggccgatgg ttcctgtggc actgccagcc tgccggtcgg tcagattatc gaacagcggc
     5941 ggcaggaggc tcagaatgcg gccacgacga tcggagcgtg ttatcatccg cccctggttc
     6001 acgatttcgg gatctattac gaaccgaaac tgctggcgaa attggcggcc gtcgttcgcc
     6061 gtgtcaagcc aaccattctg ctgctgccgg cgcccgagga ttacatggag gatcacatca
     6121 acgcctcgcg cctgatggta tcggccgcct tcacccgcgg aatggcgaac ttcaccacgg
     6181 atccccccac ggcgccctac gacgcacccg tggcgctgta ccacgccctg ccttggggct
     6241 tgctcgatcc gctgcggcgg ccggtgccgg ccgaactgtt tgtcgatatc ggttcggtgc
     6301 tcgacaagaa gaccagcatg ctggcctgcc atgtcagcca gaaggattgg ctcgcccaga
     6361 gtcagggagt cgattacctc aaactgatgc gggagatggc cgcgcaggtc ggctcgcatg
     6421 ctcgcccggg ctcatatgcc gaagggtggc gccgacattt gcatctgggc ttcgctccgc
     6481 aggactatga tccgttgtcg gaggcgctgg ctgattttct catcgggacg gttcatacgc
     6541 ccgaataaat tcacgatggt aatcccaact gaatcctcat tgtaatggag tcaaccgtca
     6601 tgccgagtca gatcattgag caagcacgca gcgcgagtcg aaacctgcag gaccgcccgt
     6661 tgagcctagt tgcgccggag tggtgggatt acacgacact cgatgcggag cttctggaag
     6721 aggcggcccg gttggatgtc gccgacatcg cgggtcttgc gcgcgacggc tttcggattg
     6781 tcttccatga cacgcttgag gaattctacc tggccgaggc gcttgaatac atcgacgcct
     6841 ggcgtcaggc cacgcccgac agcccagccg ggatttgcgg acccatcggt ccgaccgaac
     6901 aactgccgct cgtcgccgcg ttggtgaatg agctgcaact agatttgcgc cacgcctttt
     6961 tctgggggat ggatgaatgg gtgattgacg ggcgttcagt ttcaagggat caccccttgt
     7021 ccttcgctcg ggccgatatg gaactatgtt tcgatcgaat cagaccggaa ttggccatgc
     7081 cggccgaaaa tctgcatttt cccacggaag atccgaagga ttatctcagc agctgggatc
     7141 aagcccgctg cgtcgtcatg caggggggcc agggcgaggt taaacactgg gccttcaacg
     7201 atcccctgcg ccgtgagggc ccatggaagg aagcgccccc gaccgccgag gaatatctgc
     7261 ggctgagcac gcgggtggtc gagctgcatc cgctcaccct gatccagaat gcgcggactt
     7321 cgggcggcgg caatctcacg ctcgtgccga cccatgccgt gagcgtcggc ccgcgcgaaa
     7381 cctggcgagc cgaaaaggtt tcaatctggc atgccggcac gcatgacaat ccctttggca
     7441 tgcgcctgac cacattgatg atcgcgaaac aaattgtgga cactgcagtt ccgatgtcac
     7501 tgttggggtt gcatcccaat gttcagttta acttttaccg cccctgcctg ggaacctgcg
     7561 ccgtcgaaat gcactgatta tctcacgtgt gagggatccg ggcgaatctc cgcggcaggc
     7621 agatttagaa ttcccgcaaa gccggcaggg cattgcggga atttcattcc atctgcccgg
     7681 ggctattgct gaagcagggc ctcgaggcgc gcaagataat catccaatgc cgccggctcg
     7741 ggcaccgcgc gatcatagct cgcgcgcgaa tcgtcaatca gacgttgcat ttggtccagc
     7801 gtttccaagg tcttcggcgt ggcatccgaa cgattggctt ccacattggc ttcgatttcg
     7861 gccaggcgct tcagaccctc gtcgcgtatc tggagcaggg tcttgacctg cttcagcaac
     7921 ggcgtggcct gcccctcgta gtataccggt tccatctgct cgactttggc cacttcctcc
     7981 cccaggttgg ggtaaggcac gcgcaccgcc gaataattga tccagtcctg cgagaagcgg
     8041 ttgatcgcgg ccgcgttggc gacaacccag tcgcggaggt cttgcaggtc gtcggccaat
     8101 tccagcgggg cctcctggca gaagaatcga tcgtggatgc gctgttggag ttcctcaccc
     8161 gcgaagatgg gttcccatgt cagttcgcga tagacgaaac cggtcagcag gtacggcagg
     8221 cgatccatcg gataggggat ttcgtggctg taaaagtccg cggtggagta gccgggttcg
     8281 taggccggca gatagccgat gtatccctcg cgcgccgctt tggaggtcat ctccaccatt
     8341 tcccgcaacg gccgggaata aaacggcccc caatggatca tccggcgtga gatgtagacc
     8401 ccgggcacgc gctctcccag ggtggtcggg aaggtccaac tccagcgaca atccagccac
     8461 tcgaaacgtg gatcattcat gcgggtgatc tgggcataga aattcggatc gtctttgtgt
     8521 tcgtcatacc cgacgttgac gcacagaccg aaggttgggt gtttccgcca caactgttcc
     8581 gtgaactgct gcacaaagct gatcagatat tcgccgtaca tttttgagcc gtgcgagtcg
     8641 acaggaacct ggcattcatc gcactgacat tccccatgtt cgtcgcgcag ttcaaagaag
     8701 aagccatcgg cctcaggcaa cgcatccgcc atctccagga agtattccat gtgtcgctgg
     8761 cggatttcgg cattttccgg gcaaagtccc ccgaccccca cggcggcggc gtcttccaga
     8821 ccctcgccga ggtaggccac ggtcgaccac gcgaagaccc cggatcccag gtacaactta
     8881 agccccacat catgggcggc ctggatgatt tcacggatct gcccggtttc acggatgacg
     8941 gtcccctggg atgcatcccg atcaaatgtc tgcggaaatt tttcactggg gaacatcccc
     9001 gaaacccaga agtagacccg ttccatcccg tcacgcgcaa acccttcaaa aacttttctc
     9061 cagtcctcga ccgtgtactg cggcatcacg ccatagatgg ccaggcagta ggtgccgcgc
     9121 acaagcatct ggggcgagcg aaccacggaa ggggtcttga gcgagaggtc gccagcggag
     9181 ccgtcgatca gaaaatcgcg cagataatgc gccccgttca gcacaccccg ctcggtggag
     9241 gagaccacgg ccatggcccg gcgaccgtcc ggcaaatcga ccgccgccaa tgaaaagccg
     9301 tggggatcgg tgaagttcgc ccgcaacagc gcggggtggg cctgaatcag ctcgggatac
     9361 gtttcccgca gtgccgtcag cgtcgtcagc aaaatcgtcg tctcgccggc cggggcgggt
     9421 gcggcggcgg cttcagtggg tcgttcccgt ggcagaacag ttccaccgct ccaccgctcc
     9481 acggttttcg tgatcacgcc cgccgccagg tcaagacata cactcggcgg atcaagcaaa
     9541 acgatgcggg cggagggctc tccgttcgct atcaacggca acgtctcctc ggcccggagc
     9601 aatgccggga gcaccgtgat caggcccaat ccgatcacaa acctcaaact cgcagggcag
     9661 ttgaatcttg cgtcatgcat catgaagaac atggctacct cctgtgggtt aatctgttcg
     9721 aagagggctc tcagctcaat acagggaagt taaaaccttt taataaaaga gcgcgattgt
     9781 caactccatt ccgggcactg gctttcaatc gctctggcca atgcgccagt tttatttctc
     9841 cagaacgatc acaatgtcgg ccaagtcgtc agatcgggtc ggcaggctta gatatccatc
     9901 cgcatccctg gtccgcagct ccagcggttg ccccgtgtcc agcagtcgcg cgcgcgcgaa
     9961 acccaggcgg gcgccgccgg gcagcgtaag acttccactc tccggtggcg cgagcacatg
    10021 cacgtaaacg cggccgtcct tctgtgtcgt tacgccccag ggttggggcg cgatggggcc
    10081 gccgcgcgtg ccgtagatcg actcgccgtg agcggccagc cagtcgccga tggcctccag
    10141 tctctcgaca aattcaggct ggattgtgcc gtcggggcgt ggaccgatgt tgagcagaaa
    10201 attcgatccc attccggccg cccgcaccag ataatgaatc aactcgcgcg tgctcttgtg
    10261 cgcgtgatcg cccgcatgat agccccagtt gttattgatc gtctcacagg tctccagtgg
    10321 cagggcgccg atgcgggcat cctcgctgaa cccggtggtg ttggcgccgg gcagatcctt
    10381 ctcgaacatc tgaaaatcct cgcccggaaa cggctccagg tggtgattgt tgccgatcag
    10441 agccgccggt tgcagccgat gaatatgctc gtaggtctcg gtgagacgcc aatcaatctg
    10501 ggtctcggtc ggaggcgcgt ctggccgatc cagatgcttg atgcgcttga tctgctgatc
    10561 ccaccagccg tcgaaccaga tcccggccac ttcgccgtaa ttcccgccca gcagttccgt
    10621 aagctgcgcg agcatgaaat cgacgtagcg gtcgaactgg cccccttcgg cgcgacccgc
    10681 gctgtggccg gtattgccca aaggaaaata gtcaggatgg cgccagtcca ggtgtgagta
    10741 atagaagaag agtttcaggc cctggcggcg gcactcctcg gcaagcggct tgagcaaatc
    10801 cttgccatag ggcgtgctgc ggacaacatt gaattcaccc gcctgcgagt cccacaacga
    10861 gaaactatcg tgatggcgag tggtaatcgt gatgtatttc atccctgccc gcttggccaa
    10921 ggcgacccac tgtgccgggt catagcggac gggattgaat tcccgggcga acttgtcgta
    10981 ttcgacaatc gggatgccct ccacttccat gacccactca ccccgacccg gcacgctgta
    11041 gacgccccag tggatgaaca gcccgaatcg ggcgtcttca aaccaccggc gcgccgcgag
    11101 attggattcg gctggggtat aggttgcgtt tgatttagcc gacgtctcgg atggattcat
    11161 ggctgcctac ctttcctttg gtctccgcgc tgagcttcga ttcattctga cgccgcatcc
    11221 tcgggcgcca gacggtaacg gcggctctcg ccctcgggaa gctcaaagct gtaactgagg
    11281 ctccatgtcg cgttggcggg attgccgtcg gtctggcgga tgaactggcg ttccgaactg
    11341 gcctccagca cctgccagtc accttcaccc ttgacctcca gccggtaggg acccgggcga
    11401 ctcaagttgt cgatcgtcac cggccaccaa ccgtgccccg ccgtcatggt ccactcggcc
    11461 cggtcctgct ccgcacgaat cagcggcagg taggagcgtt gctggtgacc ctcgtgcacc
    11521 ttaatctcgg ggcggctgcg ggccacccaa tgcgccagcg gccaactgtc cgggtagttc
    11581 atcaattgtt cgcgcagggc acggttttcg ccgtagtaat cttgcgcctt ttgcggcaga
    11641 acgctcattt ccagccaggt ttccacgtag tctcctggct ccaggcgttc caggctggcg
    11701 gggggcgaca gctccagcac cgcgctctcg gtgcgattgc tcgtcccgta cacggcgtaa
    11761 tggatcggca catcatcccg acctcccaaa cgggcccgcc attcgcgtac gaccagcatc
    11821 cggttggccc acgccgcatg gggaaagcgg tcatcttcgg gctgagtgcc catcagcgcg
    11881 aaccaggggt tcgcgccggc gacgggttcg tttcggcgac tgtagcccgc cgcctcgccc
    11941 atcggtggcg accactgttc ggtcaggccg tcctcatcgc cgcgcgccat ggtggtgaac
    12001 aggttatcat tgtaaaagtc cgcaccgagc tgaaagaacg ccagccgttc aaattcgaca
    12061 gcctccaggc agtcatagcg tatgtgaaag accgcccgga aaaagtcgtc gctgcgccac
    12121 gaatacgtcg tcacgtcgct gcggatgcgg ccatcggcgt aggatccgcc gtagctcact
    12181 tcgctcagaa ccggcccggc cttgtgatag agcgtcttct gcccatcggg cagcaagcgc
    12241 catgtcggat agattcggtc gccgggccgc ctatcgccgg cttccaactc gcgcatttgc
    12301 tgttcgaatt gcctccggtc agcctggctt tccggggttt caaccaccag gaagtccgcg
    12361 cctcccacat ttgtcgtcca gacccatttt tgcggctctt cgctcatccc ccagacgaat
    12421 gccgggcgga tgtcgtccac caccgagcgc tgcaagttga cgctgggatc gtaacagatc
    12481 gcctcgccga aactgcccag ggcaacctgt tcccatagct ggttgccgcc ccagccgatc
    12541 aggcagagct gggcatggga gatcgccggc acgccggccc acagcgcgtt gacgcgaacc
    12601 agttccaggc tgcggtcgcc cggaggcgcc gcgagcaggg caatcgcccg tgaccagttg
    12661 cgcatcacat gccagttttt ggagatttgc accggcagcc ccaacggttc gcctccggtc
    12721 tccggatccg tcaggtgtgc gctgaagccg atcagattct ttgtggttcg gtcgttggta
    12781 ttggtatgga agtcatagtc tttctggaaa atgatccgcc cttgctgggt ggtcgccaga
    12841 tcgtggttga gcgccagcgc cgttcgctcc tccgaccaca ggtcgtcttg cgccggtaac
    12901 tccacgacgt gcgcccacag cgtcttcgaa aagctgaccg gcaggggctg gccttcagcc
    12961 gtttgagcgc gcaccccaag ccctttctct gtccgcacac gatcgctgtg tgccaacacg
    13021 cctgaacgcc ggggaacgag cacgcaggtg aacgagagcg gatcgaacgc ggcccaggcg
    13081 cccagattgc gggccacgca gggttgccca tcctcgctcc aggcgatcga ccagtcgcca
    13141 tccgccgcga acagcgccgc caggccgccc gattcgtcat tggtcgctgc ccacccggtc
    13201 acgacctggc tcatgccggg ctccgccggg cgccactcgg caggatgcga atcctgtacc
    13261 cagcgcgtgt cctgcgcctg accgacgggc agatcgatcc cgatccgcag cggacgtcgt
    13321 cccgagacca catcgtaatc cagcgtggtg ttcaggtgca gcgtcaccac catgcaatcg
    13381 ggccagcagg cgaacctgag cgaggcgttc agcccttcca gcggttggcc ttcttcatcg
    13441 gcgaatctca cgttaatgat ttcgaagtgt tgcagccagc ggccgccgtc gatcaggcgc
    13501 agcccctgcc agatcacatt gcgctcggca aggggattcc acgccccgga aacaatgcgg
    13561 tagcgtcgac ccgcgacctc catccaccag tcaagtttca ccggggccag ggtttcgagc
    13621 acatccgttt ggtgcagggc cgcggctgcc gaagaggacg caatcgcccc caatcgttcg
    13681 agccgggcgc catggacatt caacagcact ccgtactgat cggtccggac ggcatatcga
    13741 taatcggtcg gctgctccac ccgttgggga ttccgcggcc agaagctgta agtgtagccc
    13801 ttctccatcg aatcctcagc ccttgcatct cgcggcgcaa acatcgccgt cagcagcgcg
    13861 catccgcata gcagcgccat caaacgatat ccgacgctcc tgctgatctc gtgctgaacg
    13921 gttgccatga atatcttccc gctgaacatg atgataagac gcaaactacg cgaggccggg
    13981 gcctatgggc gttcagcctt ctggcgccga caccgcttcc ggtacgggaa ctaattcgtg
    14041 ttcaaagtcc tgactgatgc gcctgacggt aacccacaag gcgatgccaa acaccagcag
    14101 cgccgccccc cccacgaccg cctggcccag cagcaccttg ccgatgccga acatcaagga
    14161 ataaaccgcc agcaccgccg cgccccacga taccagcgtg cggctcaggc tttcggtctc
    14221 gtaacccggc tccgtgaagc gccggataat cggcttccag aaaaaatgga acggccgcgc
    14281 acgccggaca aattgctcca ggcgatccgc ctcggtcggc ggagtgagca gcgagaccac
    14341 taccgccaca acggcgacca ccagcatcac tgtcatcatg cgcgcgccca gcaggtcgct
    14401 gtccgagctc aggttggatt ccgggccgaa aaggtagcga gccggagcat cgaagatgcg
    14461 ggcgaacagc agcagacccg ccagcgtcca tcccgagacg gcccctgcca actcccccca
    14521 cgcgttcagg cgccaccaga accagcgcat caacccaacc agcgtgatgc tcgatcccac
    14581 cacgtaggaa atcgtcagca actgctgaat gtcgcgggcg taaagcgaga tcaccgcccc
    14641 catcgcggcc atgaagaaag tcgcggcgcg accgaccatc acgtaatgac gcgacgacgc
    14701 ttcacgtacc agaaaccgct tgtagaggtc attgacgatg tacgacgacc cccagttgaa
    14761 cagcgtcgag attgtcgaca tgaacgccgc cagcagtgcc acgatcatca cgccccgcag
    14821 tcccgtcggc atcagcgaga cgatcatccg cgggtaggcg gcgtcatggc tgaccccctc
    14881 gcccaggttc gggatcagca ggatcgagca cagcgccacg atgatccaag gccagcagat
    14941 gacggcgtag tagagcatcg tgtgcagcag catggcgagc gaagcatggc ggctatcgcg
    15001 gcaggcgagc acccgctggg cctgatatcc ccccgccaac gccacactga tccacaaaat
    15061 gccgaaatag ccaatggcgt tccagacgga catctgattg ccggccgcgc cgaccgatgg
    15121 gcggatcagc agctcactgc ccgaccactc ctccaacgct gtcagccgct caaccatcat
    15181 ctccagcccg cccaccgcgc gcacggccag gaaggcgagg atgatcgtcc ccacggtggc
    15241 cagtatgaac tggaacagat cggtatagac cacgccccac ag
//
LOCUS       DAOPCO010000069        15263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_3942_length_15263_cov_5.766899, whole genome shotgun sequence.
ACCESSION   DAOPCO010000069 DAOPCO010000000
VERSION     DAOPCO010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_15263_cov_5.766899"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /gene="topA"
                     /locus_tag="PLS90_06885"
     CDS             complement(<1..1417)
                     /gene="topA"
                     /locus_tag="PLS90_06885"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPK02167.1"
                     /translation="MAKKSTPVEKPAPRSNRDKAVGDDEAKASKARTASKRVAARKST
                     ARADAAGSVNGKNLIIVESPAKARTIERYLGSDFRVMASVGHVIDLPPTRLGVDPEHD
                     FQPEYVIIKGKKTVLDNLKKSARAARAVYLAPDPDREGEAIAHHIAEYIRDANSEIHR
                     ATFNEITKTAVRSAIEHPRPINVNLFNAQQARRVLDRLVGYKLSPLLWKKVRRGLSAG
                     RVQSVAVRICCDREREIRAFKSEEYWTIEGLVSAKLPPPFKIRLDKIDGEKAEVPNEE
                     AANRIVAEVRGKELEVGDVIKKEVAKRPFPPFITSTLQQEASRKLRMTAARTMSLAQR
                     LYEGVELGDQGSVALITYMRTDSTRLSDDALTQIRGHIRETYGPDYLPERPRAYAARK
                     EAQEAHEAIRPTDISMTPERVAPYLEKPLLALYTLIWKRAVASQIVDARLERTRIEIP
                     VGRYLFIATGSVIKFDGFLIAY"
     gene            complement(1743..2963)
                     /locus_tag="PLS90_06890"
     CDS             complement(1743..2963)
                     /locus_tag="PLS90_06890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02168.1"
                     /translation="MNRDESGHPPAQPPPAGPAAGRRPRILRAVSRMSIGGVQRGILA
                     TLARADHARFEYGVLCTKKPGHWADAVRELGVALTCQKTLPPWDPYQILRLARVLRRL
                     RPDLIHIHMAPMVIPVGTAARLAGIRHIVIQHHNDYARHWDELNLGLRRWEFLLTRRA
                     NAVIAVSRAVARMTAARVGLADGGVEVIPNGIDRETFETAAPVDPRAEWRVAPDRPLV
                     VHVARYLPTKRIEDFIEAAAIVAGRWGERHPDRPMPVFAAIGGGAPDLAEAYQRLIRH
                     HKLEGTVILAGSRPDVPGLLKAAQLGVLASEMEGFGQVVMEYLAAGLPVVATNLDCIG
                     EMLRDGEEALLVPTRAPQALADAIETLLSDAELARRLTQAGRRKLEVYDWGRAVRAYE
                     AIYDRILQGSPAAG"
     gene            3369..5852
                     /locus_tag="PLS90_06895"
     CDS             3369..5852
                     /locus_tag="PLS90_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02169.1"
                     /translation="MPLRDRPIEQKIFLLVLPACLIPAAALLILMMLGSRASLEQILG
                     DQLRQRAASLSEQFDQNLMALSERVRRAIAEDELAPGRVPADLTAMVEAVVRVNREGA
                     PVLLTPPVAGSPIPDQLDRADNVFRLLHRYRARMLPTGSFFDLQLGEPGGIQPHTYGL
                     LVHPTADGGFLVFLINARRLTEELGRRLLGLPENLIVLSDRGLLVYSTMPVSADLIDR
                     LQRRLLREQAETGALDPFIVEDGGGISLIASSPSKRLDQWRMQDGFGAGWNFLLEYDM
                     APFLGPLDALLWLSIVVSLVLVLLALGAAALASRRLVIPIKHLRRQAERLAQGDLDVR
                     VSSSGRDEIGDLAAAFNAMAARLRGTYRTLEERAEESRRHAEHIHVANEMTHAIIQAY
                     SLDSLFEILVRELGRVVPFDALWLTMNDRDGGDLNVIHIWPPGLIAHFPRGVIPLNRS
                     LHGRAVRERETLHAEIGPAQRGEFHESRALADEGFQSFLIAPLPARNRVIGTLSIAST
                     GPDAFDAELAGIVTSLAGVIAIAIEQGALFKRISQFATELERRVDDRTRELETATHKL
                     IMAEKYFATGRLAGNLAHEINNPLGIIKNYLQLVRNNLRAAEGGRRRTDPNLQSIEAI
                     NEEVDRIARLVGRMLDLHRPAEDKVEPVDLNELIAEILTLMEPNLERSRIEVVRELAH
                     ELPRPIASRDLLRQVLINLIRNAQDAMEEGGRLLLRTSAISLWEEGEERLFVRVRVSD
                     SGTGIAPEHLNRIFDPFFTTKSPDKGTGLGLCVSYSIVRMYGGNVDVESEPGRGATFI
                     VTLPVNAPATADEGPPREALIHEIHPPAD"
     gene            6121..6534
                     /locus_tag="PLS90_06900"
     CDS             6121..6534
                     /locus_tag="PLS90_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPK02170.1"
                     /translation="MGYQSMLDNFFSSQRPLFSLSQRVWNPPTDIYETEEAAIIRMEV
                     AGLDENQLNIITQGELLIVRGRRDRQLPHRKINYHLMEVHYGGFERLFAFSFPLDEKK
                     IKATYERGFLTIEVPRQAPQVTEVSVRIIEEAGPR"
     gene            6580..9153
                     /gene="lon"
                     /locus_tag="PLS90_06905"
     CDS             6580..9153
                     /gene="lon"
                     /locus_tag="PLS90_06905"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPK02171.1"
                     /translation="MSEKTDLSDVAQAIRESQHEIPEEVPLLVNTQFVLFPFMIAPLL
                     ITEERDMRKIDDVVQGTRMIGIFAPRTADGAAADGDDGEPREESAPAETDELAAINDI
                     GTVAMVLRMLRIPDGTLRLLVHGVQRVRVVEKLADGPYPRVKIEPVSEVTVDDNETQA
                     LKNLVREHLTKIVEASHSLPGDLITAANNVEDPGRLADMIASNLNLKLHEQQEILEIL
                     DTKLRLLRVQRIMNRELEIARIGSKIQTEVKTNIDRMQRDYFLREQLKAIRRELGEGE
                     DGGNELDEVEQAIEAAGMPEYALKTATKELGRLRSMSPASAEYTVSRTYVDWLVSLPW
                     NKRTEDNIDLEHARQILDEDHYDLEKVKDRILEYLAVIKLRKAIRGPILCLVGPPGVG
                     KTSLGRSVARAMGRRFVRISLGGVRDEAEIRGHRRTYIGSMPGRIIKSLKEADSNNPV
                     FMLDEIDKLGMDFRGDPASALLEVLDPEQNSTFSDHYLDMPFDLSNVMFITTANTLDS
                     IPGPLLDRMEVLRLSGYTLREKVKIARRYLIPRALENSGLSDRHVTFHDDALEQIIEN
                     YTREAGLRNLEREITNICRKAARAIAEKKRRKVRVDARHVVDALGPARFERDLLDNRT
                     REPGVVVGLAWTPTGGDILFLEATATRGSGKLTLTGKLGDVMQESVKAARTYLHAHAD
                     QFGLKEEQFSEQDLHVHVPAGAIPKDGPSAGVAMMTALASLLRGQPVAERLAMTGEIT
                     LKGAVLPVGGIKEKVLAAHRSGVQTLILPQRNEKDLVDVPAEVRQALTIHFVSTAQEV
                     LDVALPGAEGTHRSRASRARKPAQARPKNSRGVARRNGNGDRPGPAPAHARRNDRGGR
                     E"
     gene            9150..10202
                     /locus_tag="PLS90_06910"
     CDS             9150..10202
                     /locus_tag="PLS90_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02172.1"
                     /translation="MTSPAGLEDRPGSALPVTELPRDRRPDLPLLRVEGLSKLFPVRA
                     RGRFWRRAPVEYLKAVDNVSFHLHRGETLGLVGESGCGKTTTGRLILRLIEPTAGKVF
                     LDNSPDLLQTPLSRWLPYRRRMQMIFQDPYSSLNPRMTIGATIGEALTIHGLAAGRRR
                     ARVAELLEACGLPADAARRYPHELSGGQRQRVGIARALAVEPELIIADEPVSALDVSI
                     QAQIINLLEELRTRLGLAYLFIAHDLSVVGHMSHRVAVMYLGRMVELGPKDAIFGEPL
                     HPYTQALLQAVPKMDPKTRRQRLVLEGDVPSPINPPSGCPFHPRCPRAMEHCAKVVPK
                     LKEHRPGHFAACLLYD"
     gene            complement(10239..11087)
                     /locus_tag="PLS90_06915"
     CDS             complement(10239..11087)
                     /locus_tag="PLS90_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK02173.1"
                     /translation="MTAPPTDLIRLGHSPDPDDAFMFYGLAREQVDTEGLRFEHRLED
                     IETLNQRAFRGELEVTALSVHAYSHLADKYLLLNTGASMGDGYGPMVIANEPFTPEAL
                     KQKLIAIPGEHTSAYLALRLYLGDVRHRIVPFDRIFDAVRAGTVDAGLIIHEGQLTWE
                     RDGFHCIVDLGRWWQQTHEGLPLPLGVNGVRRDLGPDLCGRIDRVLRRSIAYSMEHRQ
                     AAVEYALQWGRGLDVTLADQFIGMYVNEWTQDTGGRARPAIERFLREAAARGLTPDVK
                     VEFVGD"
     gene            11648..11824
                     /locus_tag="PLS90_06920"
     CDS             11648..11824
                     /locus_tag="PLS90_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02174.1"
                     /translation="MLSIPAELNEPAGRMDDAPDAGRPDPSLPAIDPLPIEESGELEV
                     YQEEAENVIVIDSR"
     gene            11957..12901
                     /locus_tag="PLS90_06925"
     CDS             11957..12901
                     /locus_tag="PLS90_06925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK02175.1"
                     /translation="MPLPRFQPRRPLGRTGFIATALGIGDLADRAVPLDECVATVHRA
                     MDAGLNVIDTAPGYEDGYSEQIVGEALRRRPDARRSMFVIDKIDHPREPIGPQVAASL
                     GRLGLDYVDLFVFHGVSEMATWQAIAAPGGGMDQLAHAVRAGRVCWRGISSHHPDVLR
                     AAIESELCDVVLFPIGPACDPRYDDEILPLARARNVGAVCFKTFGAGKLLCDTEGYGR
                     PLRQRPRGKISSGGSGEAAAGDVLPRLSVAECLHYTLTRDPDVALLGLSFPNEQDAAF
                     AAAAAFQPLAPERMGELRARAAEALQGKGPCWWNPGGD"
     gene            12966..14681
                     /locus_tag="PLS90_06930"
     CDS             12966..14681
                     /locus_tag="PLS90_06930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK02176.1"
                     /translation="MSPKSVTTTSHAQATPSRRELLAAEHGPPARAHGPAAAGRLLRV
                     GLVYPNAYAIGMASLGFQIAYQVLEGIAGVAPERFFLDTAAQGSIETGTPLGDFDVIA
                     FSAAYEMDGPHVLDLLELAGLPLEARHRRPEAGWPLVLHGGVLVSVNRLPLYPFIDVF
                     AHGEAEGILPPVMERLRRPGVDAGSRLAALAGLPGIELTAGARAAAGLPLTAGEEPLV
                     DLLDRPLAELASLALPAPAAPPRAVAALLADAPFTSRILTPHNEFSNMALVDLARGCP
                     HHCTFCWIGHNAPAYRTRSLTEILAGIERLQPHTDRFGLVASAVGAHPDIDPLCEELM
                     ARGLRVSYSSLRVEEVSDTMLRALAAGGQRTVTIAPEAGSRRVRRLLGKRITDEQIFA
                     VTEKIFGLGAENLKLYFMTGIPSETDEEALAIAAFTEKIRAIQLRWARPRGRMGTLQI
                     NLGVFVPKPGIPLNHIEPVPLARIKSRLRKVVQALARIPNTHTAVSSPDLARAQGVLS
                     MGGVEAARYALLVREQGYDWRAANRVWRAVQPADFAWKGTGAPRVATAALHAHSSLPS
                     ARVEG"
     gene            14741..>15263
                     /locus_tag="PLS90_06935"
     CDS             14741..>15263
                     /locus_tag="PLS90_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPK02177.1"
                     /translation="MNTPRAILGIETSCDETAVALVTREGRVLTSLVASQVDLHAAFG
                     GVVPEVAARVHIQACLPLVERALSETATTWRNIEAVAVTYGPGLIGCLLVGIETAKTL
                     AWVRGRPLVAVNHLAGHLHALQLAPAVGHHLLIEGGREFSVLPVALDPPAGAPASAPP
                     PDRIARPAYPHVGL"
BASE COUNT         2386 a         5118 c         5369 g         2390 t
ORIGIN      
        1 gataggcgat caggaagccg tcaaacttga tcaccgagcc ggtcgcgatg aacaggtagc
       61 ggccgacggg gatctcgatg cgcgtccgtt ccagccgggc gtcgacgatc tgactcgcca
      121 ccgcgcgctt ccagatcagc gtgtagagcg ccagcagcgg cttttcgagg tagggcgcca
      181 cgcgttcggg cgtcatgctg atgtcggtcg gccggatcgc ttcgtgcgct tcctgcgcct
      241 ccttgcgcgc cgcgtaggcg cgcggccgct ccggcaggta atccggccca tacgtctcgc
      301 ggatgtggcc gcggatctgg gtcagcgcgt cgtcgctcag ccgcgtcgag tcggttcgca
      361 tgtaggtgat cagcgcgacg cttccctgat cgccgagctc gacgccttca tagaggcgct
      421 gcgccagcga catcgtgcgg gcggccgtca tccgcagctt gcgcgaggct tcctgctgca
      481 gcgtgctggt gatgaacggc gggaacgggc gcttggcgac ctccttcttg atgacgtcgc
      541 cgacctcgag ttccttgccg cgcacctccg cgacgatgcg gttggccgct tcctcgttgg
      601 gcacttcggc cttctcgccg tcgatcttgt ccagccggat cttgaacggg gggggcagct
      661 tggcgctgac caggccctcg atcgtccagt attcctcgct cttgaaggcg cggatttcgc
      721 gctcgcggtc gcagcagatc cgcaccgcga ccgactgcac gcggccggcc gacagcccgc
      781 ggcgcacctt cttccacagc agcgggctga gcttgtagcc caccagccgg tccagcacgc
      841 gccgggcctg ctgggcgttg aacaggttga cgttgatcgg ccgggggtgc tcgatggcgc
      901 tgcgcacggc ggtcttggtg atctcgttga acgtcgcccg gtggatttcg ctgttggcgt
      961 cgcggatgta ttcggcgatg tggtgcgcga tcgcctcgcc ctcgcggtcg gggtcgggcg
     1021 ccagatagac cgcccgcgcc gcccgcgccg acttcttcag gttgtccagt accgtcttct
     1081 tgcccttgat gatcacatac tcgggctgga agtcatgttc cggatcgacc cccagtcggg
     1141 tcgggggcag gtcgatcacg tgccccaccg aggccatgac ccggaaatcg cttcccaggt
     1201 agcgctcgat cgtgcgcgcc ttggccggcg actcgacgat gatcaggttc ttgccgttga
     1261 ccgagccggc ggcgtcggcc ctggcggtcg atttgcgcgc cgcaacccgc ttgctggccg
     1321 tgcgcgcctt gcttgccttg gcctcgtcat cgccgacggc tttgtcgcga ttcgaccggg
     1381 gggcgggttt ttcaactggg gtggactttt tggccatgag tgaatctttc ggatgcgcgg
     1441 atcaatcgct gcccgggcgg gccggagcgg tccgctgcgc ggcgggatca tcggacggtt
     1501 gtgtttcccc accgcctcgg atcggctcgt tcgcgaccgg gcgtgctcac cctatcaatg
     1561 gccttgcgcg gcgccgggtg tcaaggccaa tccgacaaga ttcgtccggc atccttcgcc
     1621 aaaagggcat aagggacaca aaagacataa aggacaaagg agcggggagg ctgctcgcaa
     1681 cgggcgttcg cccgtgcgcg aaattcggct cttcctcgtc ggtctcgcct ccccctcttc
     1741 cctcatccgg ccgcgggcga gccttgcagg atccggtcgt agatcgcctc gtaggcgcgc
     1801 acggcgcggc cccagtcata cacctccagc ttgcggcgtc cggcctgggt cagccgccgg
     1861 gccagctcgg cgtcggacag cagcgtttcg atcgcatcgg cgagcgcctg gggcgcgcgg
     1921 gtggggacga gcagcgcctc ctcgccgtcg cgcagcatct cgccgatgca gtccaggttg
     1981 gtcgccacca cgggcagccc cgccgccagg tattccatca cgacctggcc gaagccctcc
     2041 atctcgctgg ccagcacgcc cagctgcgcc gccttgagca gccccggcac atcggggcgc
     2101 gacccggcca ggatcacggt cccctccagc ttgtggtggc ggatcaggcg ctggtaggct
     2161 tcggccaggt ccggcgcccc gccaccgatt gccgcgaaaa cgggcatcgg ccggtcgggg
     2221 tggcgctcgc cccagcgtcc ggccacgatc gcggcggcct cgatgaaatc ctcgatgcgc
     2281 ttggtcggca ggtagcgcgc cacgtggacg accagcggcc ggtcgggcgc gacccgccat
     2341 tcggcgcgcg gatccacggg cgcggccgtc tcgaatgtct cccggtcaat tccgttgggg
     2401 atgacttcca cgccgccgtc ggccaggccc acgcgcgccg cggtcatccg ggcgacggcc
     2461 cgcgagacgg cgatcaccgc gttggcccgc cgggtcagca gaaactccca gcgccgcaag
     2521 ccgagattga gctcatccca gtggcgcgcg tagtcgttgt ggtgctggat cacgatatgc
     2581 cggatgccgg ccagccgcgc ggccgtcccc accgggatca ccatcggcgc catgtggatg
     2641 tggatcaggt cggggcgcag ccggcgcagc acacgcgcca ggcgcaggat ctggtagggg
     2701 tcccaggggg ggagggtctt ctggcaggtg agcgcgacgc ccagctcgcg gacggcgtcg
     2761 gcccagtgcc ccggcttctt cgtgcagagg acgccgtact cgaagcgggc gtgatcggcg
     2821 cgcgccagcg tggcgaggat gccgcgctgc acgccgccga tcgacatccg gctgaccgcg
     2881 cgcaggatgc gcgggcgccg acccgcagcc gggccggccg gcggcggttg agctggcgga
     2941 tgaccggatt cgtcgcggtt cacgctggcc aaggtagcac aggggtcgga aaaggcaatg
     3001 gggcgagtcg ccagacgatt caggcgaggg gcagggtgac cttctggctg gacataccgg
     3061 accgtcggta ctcaatttga agaagatcgt ttccgatatt ctctggtttg agccgctggg
     3121 atgaatacct gggggcgccc gtcagcggga cggcgcgagc attccctgcc atcctgacca
     3181 ggggtttggt tcgtcgcggc ggtttcccga aatgacttgg cccgccttgg gctgtatggc
     3241 agaatactct cgattcagga cttaccccac gccgggccgg aacgggccgc ccccgcccgc
     3301 cgcattgctc cgggtcggcg ggccgtcgcg cctcgcggtc ctgacaccgg tccaccccag
     3361 gcagcatcat gccgcttcgc gaccgcccca tcgagcagaa gatcttcctg ctggtcctgc
     3421 cggcctgcct gatcccggca gcggcgctgc tcatcctgat gatgctcggc tcgcgcgcga
     3481 gcctggagca aatcctgggc gatcagttgc gccagcgggc ggcctcgctc tccgaacagt
     3541 tcgaccagaa cctgatggcg ctgtcggaac gggtgcgacg ggcgatcgcg gaggacgagc
     3601 tcgctccggg ccgcgttccg gcggacctga cggcgatggt cgaggccgtc gtgcgggtca
     3661 accgcgaggg ggcgccggtc ctgctgaccc ccccggtggc gggttcgccg atccccgatc
     3721 agcttgatcg ggccgacaat gtctttcgcc tgctccaccg gtatcgcgcg cggatgctgc
     3781 cgacgggctc cttcttcgac ctgcaactgg gcgagccggg cgggatccag ccgcacacct
     3841 acggtctgct ggtccacccg acggccgacg gcggctttct ggtcttcctg atcaacgccc
     3901 ggcggctgac ggaggaattg gggcgccggc tgctgggttt gcccgagaac ctgatcgtcc
     3961 tgtccgaccg cgggctgctg gtttattcca cgatgccggt cagcgcggac ctgatcgacc
     4021 ggctccagcg gcggctgctg cgcgagcagg ccgagacggg ggcgctcgac cccttcatcg
     4081 tcgaggacgg aggcggcatc tcgctgatcg cctcgagccc gagcaagcgg ctcgaccagt
     4141 ggcggatgca ggacggtttc ggggcgggct ggaattttct gctcgagtac gacatggcgc
     4201 cgttcctggg tccgctggac gcgctgctct ggttgtcgat cgtcgtcagc ctggtgctgg
     4261 tgctgctggc gctgggggcc gcggcgctgg cctcgcgccg gctggtcatc ccgatcaagc
     4321 acctgcgccg ccaggccgaa cggctggcgc agggcgatct ggacgtgcgc gtttcgtcga
     4381 gcgggcggga cgagatcggc gatctggccg ccgcgttcaa cgccatggcc gcccgcctgc
     4441 gcggcaccta ccgcacgctg gaggagcggg ccgaggagag ccgccgccac gccgaacaca
     4501 tccacgtcgc caacgaaatg acccacgcga tcatccaggc ctacagcctg gacagtctgt
     4561 ttgagatcct ggtgcgcgag ctgggccggg tggtcccgtt cgacgcgctg tggctgacga
     4621 tgaacgaccg cgacggcggg gatttgaatg tgattcacat ctggccgccc ggcctgatcg
     4681 cccatttccc acgcggcgtg atcccgctca accggtcgct gcatggccgg gcggtgcgcg
     4741 agcgcgagac gctgcatgcg gagatcgggc cggcgcagcg cggggagttt catgaatcgc
     4801 gcgcgctggc cgacgaagga ttccagtcgt tcctgatcgc gccgctgccg gcgcgcaata
     4861 gggtaatcgg cacgctgtcg atcgcctcga ccgggccgga cgcgttcgac gcggaactgg
     4921 ccggcatcgt gacgtcgctg gcgggggtga tcgccatcgc gatcgagcag ggggcgctgt
     4981 tcaagcgcat ctcgcagttc gccaccgagc tggagcggcg ggtcgacgac cgcacgcgcg
     5041 agctggagac ggcgacacac aagctgatca tggccgagaa gtatttcgcc acggggcggc
     5101 tcgccggcaa tctggcgcac gagatcaaca acccgctggg gatcatcaag aattacctgc
     5161 agctcgtgcg caacaatctg cgcgcggccg aggggggacg gcggcgcacc gaccccaatc
     5221 tgcagtcgat cgaggcgatc aatgaggagg tcgaccgcat cgcccggctg gtcggccgga
     5281 tgctggacct gcaccgcccc gccgaggaca aggtcgaacc ggtcgacctg aatgaattga
     5341 tcgccgaaat cctgacgctg atggaaccca atctggaacg cagccggatc gaggtcgtgc
     5401 gggaactggc gcatgaattg ccgcggccga tcgcctcgcg cgacctgttg cgccaggtcc
     5461 tgatcaatct gatccgcaat gcccaggacg cgatggaaga gggggggcgg ctgctgctgc
     5521 gaacctcggc gatcagcctg tgggaagaag gggaggagcg gctgttcgtc cgcgtccgcg
     5581 tcagcgactc ggggacgggc atcgcccccg aacacctcaa tcggattttc gatccgttct
     5641 tcacgaccaa gtcgcccgac aaggggaccg ggctggggct gtgcgtctcg tacagcatcg
     5701 tgcgcatgta tggcggcaac gtggatgtgg agagcgagcc gggccgcggc gccacgttca
     5761 tcgtcaccct gccggtcaac gcgccggcga ccgccgacga aggtcccccg cgcgaggcgc
     5821 tgatccacga aatccacccg ccggcggact gaacggcaaa gtcccgcgca cacgggcggc
     5881 gacatggccc ggcggttgcg agggtatgcg tacctgcgca cgcctggtcc cgcgcgcggg
     5941 cggcgccagg ggacgaaggt tgcgcgccgc cggatgaagc cggataatca tgggactgga
     6001 cctgaccggc caaagcgagc ctggcggctg cgcgcagtgg gcggcggtgc atgcggaatc
     6061 ggccgggcct ggttgaccgg ccgcgcgcga gcctggcggc ccccacggga ggagcgagcg
     6121 atgggctatc agtcgatgct ggacaatttc ttctcctcgc agcgcccgct gttctcgctg
     6181 agccagcggg tctggaatcc gccgaccgat atctatgaaa ccgaggaggc ggccatcatc
     6241 cggatggaag tggccgggct ggatgagaat cagctcaaca tcattaccca gggggaactg
     6301 ctgatcgtcc gcgggcggcg cgaccgccag ctcccccacc gcaagatcaa ctaccacctg
     6361 atggaagtgc actacggcgg cttcgagcgg ctgtttgcct tttcgtttcc gctcgatgag
     6421 aaaaagatca aggcgacgta cgagcgcggg tttctgacga ttgaagtgcc gcgacaagcc
     6481 ccgcaggtga ccgaagtgtc ggtccggatc atcgaggagg cgggaccgcg ctgaaggccg
     6541 ggcccgctcc gcccccaaac aacgcgcagg tagtcgataa tgagtgagaa aaccgatttg
     6601 agcgacgtgg cccaggcgat ccgggagagc cagcacgaga ttccggagga agtcccgctg
     6661 ctcgtcaaca cgcagttcgt cctgtttccg ttcatgatcg ccccgctgct gatcaccgag
     6721 gagcgggaca tgcggaagat cgacgacgta gtgcagggga cgcggatgat cgggatcttc
     6781 gcgccgcgga cggccgacgg cgccgcggcc gacggggacg acggcgaacc gcgggaggag
     6841 agcgcgccgg ccgagaccga tgagctggcc gcgatcaacg acatcggcac cgtcgcgatg
     6901 gtcctgcgga tgctgcggat ccccgacggg acgctgcggc tgctggttca cggcgtgcag
     6961 cgcgtgcgcg tcgtcgagaa actggccgac ggcccgtatc cgcgggtaaa gatcgagccg
     7021 gtgagcgaag tgaccgtcga tgacaacgaa acgcaggcgc tgaagaacct cgtccgcgaa
     7081 cacctgacca agatcgtcga ggcgagccat tcgctgccgg gcgacctgat cacggcggcg
     7141 aacaacgtgg aggatccggg ccggctggcc gacatgatcg cctccaatct gaacctcaag
     7201 ctgcacgaac agcaggagat cctcgagatt ctggatacca agctgcggct gctgcgggtg
     7261 cagcggatca tgaaccgcga gctggagatc gcgcggatcg gctcgaagat ccagaccgag
     7321 gtcaagacca acatcgaccg gatgcagcgc gactactttc tgcgcgagca gctcaaggcg
     7381 attcgccgcg agctgggcga gggcgaggac ggcggcaatg aactggacga ggtggagcag
     7441 gcgatcgagg ccgccgggat gccggaatat gcgctcaaga cggccaccaa ggaactcggc
     7501 cggctgcgct cgatgtcgcc cgccagcgcc gaatacaccg tcagccggac ctatgtggac
     7561 tggctggtga gcctgccgtg gaacaaacgg accgaggaca acatcgacct ggagcacgcg
     7621 cgccagatcc tggacgagga ccactacgat ctggagaagg tcaaggatcg gatcctggaa
     7681 tatctggcgg tgatcaagct gcgcaaggcg atccgcgggc cgatcctgtg cctggtgggg
     7741 ccgccgggcg tggggaagac gtcgctgggg cgctcggtgg cgcgcgcgat ggggcgccgc
     7801 ttcgtgcgca tcagtctggg gggcgtgcgc gacgaggccg agattcgcgg gcaccggcgc
     7861 acgtacatcg gctcgatgcc ggggcggatc atcaagtcgc tgaaggaagc cgattcgaac
     7921 aacccggtgt tcatgctgga tgagatcgac aagctgggga tggactttcg cggcgacccc
     7981 gcctcggcgc tgctcgaagt gctcgatccg gagcagaatt ccacgttcag cgaccattac
     8041 ctggacatgc cgttcgacct gtccaacgtg atgttcatca cgacggccaa cacgctcgat
     8101 tcgatcccgg ggccgctgct cgatcgcatg gaggtgctgc ggctgtcggg ctacacgctg
     8161 cgcgagaagg tcaagatcgc ccggcgctac ctgatcccgc gcgcgctgga aaattcgggg
     8221 ctctccgacc gccatgtgac gttccacgat gacgcgctgg aacagatcat cgagaactac
     8281 acgcgcgagg cggggctgcg caacctggag cgggagatca ccaacatctg ccgcaaggcg
     8341 gcgcgcgcga tcgccgaaaa gaagcggcgc aaggtgcggg tcgacgcgcg gcacgtcgtc
     8401 gacgccctcg gaccggcgcg atttgagcgc gacctgctgg acaaccgaac gcgcgagccg
     8461 ggcgtggtcg tcggcctggc ctggacgccg accggcggcg acatcctgtt cctggaggcg
     8521 acggccacgc gcggcagcgg caaactgacg ttgaccggca agctcggcga tgtgatgcag
     8581 gaatcggtca aggcggcgcg gacctacctg cacgcccacg ccgatcagtt cggtctgaag
     8641 gaagagcagt tcagcgaaca ggatctgcat gtgcacgtgc cggcgggggc gatcccgaag
     8701 gatggaccgt cggcgggcgt ggcgatgatg acggcgctgg cgtcgctgct gcgcggtcag
     8761 ccggtggccg agcggctggc gatgacgggc gagatcacgc tgaagggggc ggtgctcccc
     8821 gtcggcggca tcaaggagaa ggtgctggcg gcgcaccgca gcggggttca gacgctgatc
     8881 ctgccccagc gcaatgaaaa ggacctggtg gacgtgccgg ccgaggtgcg ccaggcgctg
     8941 acgatccact ttgtcagcac ggcgcaggag gtgctggacg tggcgctgcc gggcgccgag
     9001 gggacgcatc gctcgcgcgc cagccgggcg cggaaaccgg cgcaggcgcg gccgaaaaac
     9061 tcgcgcggcg tcgcgcggcg caacggcaac ggcgaccggc ccggcccggc gccggcccac
     9121 gcgcggcgca acgatcgcgg aggccgcgaa tgacatcgcc ggcgggtctc gaggatcgcc
     9181 ccggcagcgc gctccccgtg acggagctgc cgcgcgaccg gcggcccgac ctgccgctgt
     9241 tgcgcgtcga ggggctgagc aagctgtttc ccgtccgcgc gcgcggccgc ttctggcgtc
     9301 gcgcgcccgt ggaatacctc aaggccgtcg acaacgtcag cttccatctg caccggggag
     9361 aaaccctggg gctggtgggc gaaagcggct gcggcaagac caccaccggc cggctgatcc
     9421 tgcggctgat cgaaccgacc gccggcaagg tcttcctgga caactcgccc gacctgctgc
     9481 agacgccgct cagccgctgg ctgccctacc ggcggcggat gcagatgatc tttcaggatc
     9541 cctatagttc gctcaatccg cggatgacca tcggcgcgac gatcggcgag gcgctgacga
     9601 tccatggcct ggcggccggg cgccggcgcg cgcgcgtggc cgagctgctc gaggcctgcg
     9661 ggctgccggc cgacgccgcc cggcgttacc cccacgagct gagcggcggc cagcgccagc
     9721 gggtcggcat cgcccgcgcg ctggcggtcg agcccgagct gatcattgcc gacgagccgg
     9781 tctcggcgct cgacgtctca atccaggccc agatcatcaa cctgctggag gaactgcgca
     9841 cccggctggg gctggcctac ctgttcatcg cgcacgacct gagtgtcgtc ggccacatga
     9901 gccatcgcgt ggcggtgatg tatctggggc ggatggtgga actggggccg aaggacgcga
     9961 ttttcggcga gccgctgcat ccctacacgc aggcgctgct gcaggcggtg ccgaagatgg
    10021 acccgaaaac gcggcggcaa cggctggtgc tcgagggcga cgtgccgagc ccgatcaatc
    10081 cacccagcgg ctgcccgttt catccgcgct gcccccgcgc gatggagcac tgcgccaagg
    10141 tggttcccaa gctgaaggag caccggccgg ggcacttcgc ggcgtgcctg ctctacgatt
    10201 gagcgccggc cgcgcggcgc ggcgggacgc attgcggctc agtcgccgac gaactccacc
    10261 ttcacatccg gcgtgaggcc gcgggcggcc gcctcgcgca ggaaccgctc gatcgccggc
    10321 cgcgcccgtc ctcccgtatc ctgcgtccat tcattgacat acatgccgat gaactggtcg
    10381 gccagcgtga catcgagacc gcggccccac tgcagggcat attcgaccgc cgcctggcgg
    10441 tgttccatgc tgtaggcgat cgagcggcgc agcacgcggt cgatccggcc gcacaggtcg
    10501 gggccgagat cgcggcggac gccgttgacg cccagcggca gcggcaggcc ctcgtgcgtc
    10561 tgttgccacc agcggcccag gtcgacgatg cagtgaaagc cgtcgcgttc ccaggtgagc
    10621 tggccctcgt ggatgatcag gccggcatcg accgtgcccg cccggaccgc gtcgaaaatg
    10681 cggtcgaacg gcacgatgcg gtgccggacg tcgccgagat agagccgcag cgccaggtag
    10741 gcgctcgtat gttcgcccgg gatcgcgatc agcttctgct tgagcgcctc gggcgtgaac
    10801 ggctcgttgg cgatcaccat cgggccgtag ccgtcgccca tgctggcgcc ggtattgagt
    10861 agcaggtatt tgtcggccag atgcgaatag gcgtggaccg agagggcggt cacctccagt
    10921 tcgccgcgga aggcgcgctg gttgagggtc tcgatatcct cgagccgatg ctcgaaacgc
    10981 aggccctcgg tatcgacctg ctcgcgggcg aggccgtaga acatgaaggc atcgtcggga
    11041 tcggggctgt ggcccaggcg aatcagatcg gtgggggggg cggtcatggc gatctcctct
    11101 gtcgtcgctc gcggccgaaa ccgcgacgga agcttaccag tgtagcatcg cggcggcgcg
    11161 atggaagtct tagatggcct cttcggaagg ccggatcgtg ccacccggca ttgggcggcg
    11221 tttggcccga gcagggagcg gagtcgaggc agctcttgtg gagtgcggtg gcagagcgtc
    11281 gcagtgcaac gcgtcgacac cgctttggat ccgatgaccc gggccgatgc tgaccaatcc
    11341 aaagcggcgt cgccaggcgc tgcgcgcttt ctgccgctcg catgacgtgc tggcattgtc
    11401 accggcgcaa ttctaatctg tggaccatcc ttcacgttcg ggttttcgcg agccggggac
    11461 gaaggatgag agccgccttt gggagcggtc acgaggacga ggacgaaaac gagagacgga
    11521 atctggagga gggaggcaac ggtgggtcgc cgtggcacga acagcgtcca actaattggc
    11581 tggacatgag ctttccaaac cgctttatct gaaagtggga gtgccgcgct gaaactggaa
    11641 agcgcaggtg ctgagcatcc cggccgagct gaacgaaccg gccggccgga tggatgatgc
    11701 gcccgatgcc ggccggcccg atccgtccct cccggccatc gacccgcttc ccatcgagga
    11761 gagcggcgag ctggaggttt accaggaaga ggcggagaac gtgatcgtca tcgacagcag
    11821 gtaaaccgga tggacgatct gtcgatcccg atcccgatag ggagagatac cgggaggtgg
    11881 cgcgccgagc cgccggtgcg ccataatcac agcgcgttgt ccgcgctgaa ctgccctcag
    11941 gccaaggacc ggactcatgc ccctcccccg tttccagccg cgccgcccgc tgggccgcac
    12001 cgggttcatc gccacggcgc tcggcatcgg cgatctggcc gatcgcgccg tgccgctcga
    12061 tgagtgcgtc gccaccgtgc atcgcgccat ggatgccggg ctgaacgtga ttgacaccgc
    12121 ccccggctac gaggacggct acagcgagca gatcgtcggc gaggcgctgc ggcgccgccc
    12181 cgacgcgcgc cggtcgatgt tcgtcatcga caagatcgac cacccgcgcg aacccatcgg
    12241 gccccaggtg gcggccagcc tcggccggct cgggctcgac tacgtcgacc tgttcgtttt
    12301 ccacggcgtc tcggagatgg cgacgtggca ggcgatcgcc gcgcccggcg gcggcatgga
    12361 ccagcttgcg cacgcggtcc gggcgggtcg cgtctgctgg cgcgggattt ccagccacca
    12421 ccccgacgtc ctccgcgccg cgatcgaatc ggagctgtgc gatgtggtcc tgttcccgat
    12481 cggcccggcc tgcgacccgc gctatgacga cgaaattctc ccgctcgccc gcgcgcgcaa
    12541 tgtcggcgcc gtctgcttca aaaccttcgg cgccggcaag ctgctgtgcg ataccgaggg
    12601 ctacggccgg ccgcttcggc aacgcccgcg cggcaaaatc agttccggcg gaagcggcga
    12661 ggcggccgcg ggcgacgtcc tgccgcgcct gagcgtcgcc gagtgcctgc attacacgtt
    12721 gacccgcgac cccgatgtcg cgctgctggg cctgagcttc cccaacgagc aggatgccgc
    12781 gttcgccgcc gccgcggcat tccagccgct cgcgccggag cggatgggcg agctgcgcgc
    12841 gcgcgccgcc gaagccctcc agggcaaggg cccctgctgg tggaaccccg gcggcgactg
    12901 aaccgaccga gaccgccgcg ccgtcgcccg ttgcccctcg cgggggtttc cggtaaactc
    12961 gtcgggtgag ccccaagtcc gttaccacca catcccatgc ccaggcgacg cccagccggc
    13021 gcgagctgct ggcggccgaa cacggcccgc cggcgcgggc gcacggtccg gccgccgccg
    13081 ggcggctcct gcgcgtcggc ctcgtctacc cgaacgccta cgcgatcggc atggcgagcc
    13141 tcggcttcca gatcgcctat caggtgctgg aggggatcgc cggcgtcgcg cccgagcgtt
    13201 tttttctcga caccgccgcg cagggctcga tcgaaacggg cacgccgctg ggcgacttcg
    13261 acgtgatcgc cttctccgcc gcctacgaga tggacggccc gcacgtgctc gacctgctgg
    13321 agctggccgg cctgccgctc gaggcgcgcc atcgccgtcc cgaagcgggc tggccgctcg
    13381 tgctgcacgg gggagtcctc gtgtcggtca accgcctgcc gctctacccc ttcattgacg
    13441 tctttgccca tggcgaggcc gaagggattc tgcccccggt gatggaacgg ctgcgccggc
    13501 cgggcgtgga tgccgggagc cggctggcgg cgctggcggg tctcccgggc atcgaactga
    13561 ccgccggcgc ccgggccgcg gccggcctgc cgctgaccgc gggggaggag ccgctcgtcg
    13621 atctgctgga ccggccgctg gcggaactgg cgtcgctcgc cttgccggcg ccggctgccc
    13681 cgccgcgcgc cgtcgccgcc ttgctggccg atgcgccgtt cacctcgcgg atcctgacgc
    13741 cgcacaacga attctccaac atggcgctgg tcgatctggc gcgcggctgc ccgcatcact
    13801 gcacgttctg ctggatcggc cacaacgcgc cggcctatcg cacgcgctcg ctgacggaga
    13861 tcctggccgg gatcgaacgg ctgcaacccc acaccgaccg gttcggcctg gtcgcctcgg
    13921 cggtcggcgc gcaccccgac atcgacccgc tgtgcgagga gctgatggcg cgcggcctgc
    13981 gcgtttccta ctcctcgctg cgcgtggagg aagtctccga cacaatgctg cgggcgctgg
    14041 cggccggcgg gcagaggacc gtcacgatcg cgccggaggc cggcagccgc cgcgtccgcc
    14101 gtctgctggg caaacgcatc accgatgaac agatcttcgc ggtgacggag aagatcttcg
    14161 gcctgggcgc cgaaaacctg aagctctact tcatgaccgg gatcccgtcg gaaactgacg
    14221 aggaggcgct ggcgatcgcc gcgttcaccg agaagatccg cgcgatccag ctgcgctggg
    14281 cgcggccccg cggccggatg ggaacgctcc agatcaacct gggcgtgttc gtgccgaaac
    14341 cggggattcc gctcaatcac atcgagccgg tgccgctggc gcggatcaag tcgcgcctgc
    14401 gcaaggtggt ccaggcgctg gcgcgcatcc caaacacgca cacggcggtc tccagtcccg
    14461 atctggcgcg cgcccagggc gtcctctcga tgggcggcgt cgaggcggcg cgctatgcgc
    14521 tgctcgtgcg cgaacagggc tacgactggc gcgcggcgaa ccgggtctgg cgcgccgtgc
    14581 aaccggccga cttcgcctgg aaggggaccg gggcgccgcg cgttgcgacc gccgccctgc
    14641 atgcgcattc ctcattgccc tccgcgcgcg tggagggatg atatccgtcg gatcggtcgg
    14701 atccgacgga cttcgagagg acgagggacg actcttaggg atgaacactc cgcgggcgat
    14761 tctcggcatc gaaacctcgt gtgacgaaac ggccgtcgcg ctcgtcacgc gcgaggggcg
    14821 cgtgctcacc tcgctcgttg cctcgcaggt cgatctgcat gcggccttcg gcggcgtcgt
    14881 tcccgaagtg gcggcgcgcg tccacatcca ggcctgtctg ccgctcgtgg aacgcgcgct
    14941 gagcgagacc gccacgacct ggcgcaatat cgaggcggtc gccgtgacct acggccccgg
    15001 cctgatcggc tgtctgctcg tcggcattga gaccgccaag acgctggcct gggtgcgcgg
    15061 gcggccgctc gtggcggtca atcacctggc ggggcatctg cacgcgctgc aactggcgcc
    15121 cgccgtcggc caccatctgc tgatcgaagg ggggcgtgaa ttctcggtgc tgccggtggc
    15181 gctcgatccg ccggccggcg cgccggcctc cgccccgccg cccgaccgca tcgcccggcc
    15241 ggcctatccg cacgtcggcc tga
//
LOCUS       DAOPCO010000070        15065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4001_length_15065_cov_5.058694, whole genome shotgun sequence.
ACCESSION   DAOPCO010000070 DAOPCO010000000
VERSION     DAOPCO010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4001_length_15065_cov_5.058694"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLS90_06940"
     CDS             complement(<1..393)
                     /locus_tag="PLS90_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPK02178.1"
                     /translation="MDQLQLELKPREVGPKRANRRLRRGGQVPAVVYGAGKEPAMVAI
                     DNHAIEMILHGATGTNKLFNIQLNGEQQQALIRDVQRHPVNNRILHIDLMRVDMDKLI
                     DVEVSVHAVGADPEGVRAGGILEHLVRSV"
     gene            complement(568..3300)
                     /locus_tag="PLS90_06945"
     CDS             complement(568..3300)
                     /locus_tag="PLS90_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPK02179.1"
                     /translation="MGPRQLVRLLVLVLSLSLGVSCARPWLAGPAPGFTGAPETIDAF
                     ERAAAYQFGESRAALSEIEEMLAGPRVRAEQNRQLEDNLIALLATGTPDARRFAAREL
                     GRIGSDRAVRELARRLADPELSHMARHALQAIPGDAAARALLKGMKKTAGELRIGMIN
                     SLGDRGEVMAVGPLTRLADDASTGVAAAAIAALGKIDSAAAAEALMELPIRTLRPEPR
                     RELQTALLRVAERKLGQGAAADAAAIYERLLAAPETQAGRIAALRGLMLARGIDARPQ
                     LDEILRGGDPAMQAQAAGFLRELAGSDLTRALLDDYDAYPAGARAALLRAAAADPAID
                     ARPWLEHGLESDDEAVRIAALEGLGARGDAESVGRLVGRAARGEGEEADTARRVLSRI
                     AGEGVDGAFLEVLAQGENAERLAVIAALRARGATQAVPELIAQAESDDAEVRKAALAA
                     LGELGREEHLDDLLDLLERIDPDGQAAAGAAVAEVARRLGDPEAAARPVLEKYESART
                     AELRARLLPVLGRLGGESALRAVRGATGAEQDRVREAAVRALAEWPSPAAMPDLLRLA
                     RQAEDRRERVLTLRGYIRLIEQIGDENEDEGQDADLSARRLEPYGQAMAAAERYEERR
                     QVLAGVAELADPAALAFLRPCLDDAQLRDEALAAEIKVLGAIAGRDAELAASEADNLL
                     DRVEAGQIRLKAELAEVILQAAGANAGARPEVAGELIDRVEQAAGEDAAIRERIQASR
                     EFIEQLTPMLAAWQVAGPYEKPGASQEELLAEPFAPETPAGTDEAAWRIMPTVVWRMP
                     GAVSLGEAIGGNHRVAYLRTWVWSPRAMAARLELGSDDGIKVWLNGEPVFEHPVMRGL
                     EAGADEAPVELRAGWNALMLKVTQATAGWGAIARLTTPDGAPIAGLRTSPRPPE"
     gene            complement(3374..4678)
                     /locus_tag="PLS90_06950"
     CDS             complement(3374..4678)
                     /locus_tag="PLS90_06950"
                     /inference="COORDINATES: protein motif:HMM:NF039847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02180.1"
                     /translation="MPPKSLNRRQFLQRSLGAAAGLGALASIGRVHAAPPPPPGERIT
                     MGAIGLGAMGEADMAAFLHMPPVQMVALCDVDAQRARAALNTVNEYYGERTWSGRYAG
                     CHVYHDYRELLAREDLDAVVIALPDHWHALATVHAARAGKDIYGEKPLSLTIEQGRIM
                     SDEVRRHGCVFQTGSQQRSDRLFRFACELVRNGRIGRLHNMRVGLPTAPVGEVVQPQP
                     VPEHFDYDFWLGPAPWAPYHERRCHWDFRWIFDYSGGQVTDWGAHHCDIAQWGKGTEL
                     TGPVKVEPVAWERPRDGLWDVFTHYRFICTYDDGVTMDVSDGYPNGVRFEGDAGWVFV
                     TRGAIEAEPAALLDEPIGPGEIQLYQSEDHRADFLRSVRTREEPIAPIEVAHRSITIA
                     HLGNIAMLTGRTIRWDPDAERIVGDEEAQRLCSRAMREPWRL"
     gene            4991..6406
                     /locus_tag="PLS90_06955"
     CDS             4991..6406
                     /locus_tag="PLS90_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK02181.1"
                     /translation="MSHSGHDASDASPWVLVADDDPTIRLSLKHTLERAGLRVLTAAD
                     GREAIEQMTEEISAAILDLKMPGRNGLECLTHFQQHFPDVQVVMITATNDIRDAVEAM
                     KLGAYDYITKPFSPDELIRLLRKAVAAADLRRENRHLRSALGESVSSTTLLGQSLAMR
                     QLRQMIGKVSRLDSTVLLLGESGVGKSLVARCIHRQSPRAAGPFVTVNCTAMPRELME
                     SELFGHERGAFTGALERRLGRLEMAQRGSLFLDEIGDLPIDLQPKLLSFLQERVFHRV
                     GGSQEMRSDVRIIAATNHDLRDMVNAKMFREDLYYRLNVLPITIPPLRDRREDLEELT
                     TNLLARIARNRGARPCRLSDGARRALLAHDWPGNVRELENALERATAFCENFTVMPED
                     LPLEIRAQGRPGADGPAAEPQHWLGGMSLDDVERLAIEQTLVHCRGNKAEAARTLRIS
                     EKSIYNKMKRLGLNGHPRPSL"
     gene            6772..7833
                     /locus_tag="PLS90_06960"
     CDS             6772..7833
                     /locus_tag="PLS90_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK02182.1"
                     /translation="MSGRQSFLGLSSIALLSDAETRSISAENPTGEKGGGAQAEPGSY
                     NVALGKGWKCRPAITLEQHATTTLADIQGPGTIQHIWITVDTKAYRDCVLRFYWDNEE
                     TPSVEAPLGDFFAVGHGLRYNLNSLPVAVNPNGGFNCYWPMPFRERARITIENQRAEA
                     IHSFFYQITYELAPVPREAAYFHAQWRRKVVDLDYPEYTILDGVKGQGQYVGTFMALQ
                     QTADGWWGEGEIKFFIDGDSEYPTICGTGTEDYFGGAWGFGDTFSTPFLGYPLWWKEP
                     GAVPRHALYRWHIPDPIRFKQDLRVTIQNLGGWPDRPVFLARFDDIASTAYWYQTEPH
                     APFPEFPSLYQRWTRNWNG"
     gene            complement(8184..9194)
                     /locus_tag="PLS90_06965"
     CDS             complement(8184..9194)
                     /locus_tag="PLS90_06965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HPK02183.1"
                     /translation="MLAYETQIAIVGSGPAGLAAALTLGRAGYDTLILERGRFAHGVA
                     NWPVYMKLFSTTDLVELCGYPLAIPDEKPDRRQYLRYLQRFATDHGLAFQLRHEVRGV
                     EGEDGRFIVRGVAGGDVPFEARCQKVVLATGAYDHPAALNVPGEDLPKVSHYYTEVND
                     YFGRKVLIVGGRHSAAETALELYRAGVEVTICHRRAEFSGLKYWIAPDLANRIKEGGI
                     RAIMPAHVREIRPDSVVIQHETEGPLEIENDAVLALTGYHPDPEFLGRLTLPVDEERK
                     QPVFDPRTFESPRPGIYMIGVMLAGNISGAIFIENSRYHGERVLEHLRQRGFKPAAEP
                     GA"
     gene            complement(9596..10321)
                     /locus_tag="PLS90_06970"
     CDS             complement(9596..10321)
                     /locus_tag="PLS90_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02184.1"
                     /translation="MIPTPAAPPPFPPLNDPLVSVIMPIYNERELIREVLRRVRAQPF
                     RKQMILVDDHSTDGTWELLCEEEGSRPDTVLVRHEVNHGKGMAIRSGLKHAAGEIILI
                     QDADLEYDPAELPRLLEPIVAGRASVVYGSRFLGRIEKMRLPNRVANYLLAWSVSLLY
                     GQRITDEATAYKIFRREVIQAIDLTCRRFEFCPEVTAKVLRRGERIVELPVRFTARTW
                     EEGKKIGWRDFFQAMWVLVKCRF"
     gene            10519..10812
                     /locus_tag="PLS90_06975"
     CDS             10519..10812
                     /locus_tag="PLS90_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02185.1"
                     /translation="MTSSDLPLAERLKRQSHRICTEILQSDVEWVDIAIQIERMRAMC
                     EAEAPDKLELFEALYVARFTRLWQQWREPEERAARPPIAANPIIEGEWRETRG"
     gene            10938..12575
                     /locus_tag="PLS90_06980"
     CDS             10938..12575
                     /locus_tag="PLS90_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPK02186.1"
                     /translation="MSELFLNQRRLDIQPGATLFDAADLLGLKLPSSCGRHGSCHECI
                     IQVTAGEEALNPRTENEDFLRGGYRLACQCRLERPEPVVHAHALRRGTPQIAASGRPV
                     PVALAPAVTRGPDDTVLLDGEPIAQRRGPLLGAAADIGTTTVVLRLVDLETGQIVAAQ
                     SFENPQMFGGGDVMGRITYDRETKRRDLQQVLVAYLNRSLGEFCEALGAETDDIYEFV
                     AVGNATMRDLLFGYDVQTIGVKPFMSVSEIEFREGQRETTALACRPTRLRLAINRKGR
                     VWGAPLIACHVGADTAACVAAVDVERAARPVIIMDIGTNTELVVGHGERVLCASCPAG
                     PAFEGARVTWGMPGLEGAIESVRLENGQVDYRVIGDAAPAGICGSGMIDALGELLRTG
                     RMNMLGRLEDGDGRFLIAEAAGIYLSNKDISELAQAKGANIAGILILLRELGLTLEEV
                     DRFYLAGGFAQYIDIEHAIRIGMLPRMPVEKFEKLGNAAIEGATALLCSTDLRRRLDA
                     YVHRIGHVELENHPAFFGYYVEGMQFKASDEMAPAGV"
     gene            12744..13730
                     /locus_tag="PLS90_06985"
     CDS             12744..13730
                     /locus_tag="PLS90_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02187.1"
                     /translation="MLRFLLRRLFHGLLILLGVSFLTFFLINQSPGDFLDRKRLDPQV
                     PREFIEAEIQRLHLDQPWPVVYCFWLKDIVTRLDFGRSFEYKQPVFDVLAGRVLNTLI
                     LSFCSVVFAWMLAIPLGIIAGYRQYSLVDKTASAVAFLGVSVPNVFLALLAIFLAART
                     GWFPTGGLTDQRNWPTMSTAERMLDMAHHLILPTIVLGTGMMAVLMRQMRGQLLDVLQ
                     ADYIRTARAKGVGERALLLKHAVRNAINPLITIFGFSLSTLLSGSVLVEKVMSYPGLG
                     RLTVDAMFNKDIYLVMASVLVATAMLVLGNLIADLLLAWSDPRIKLEDRATS"
     gene            13727..14896
                     /locus_tag="PLS90_06990"
     CDS             13727..14896
                     /locus_tag="PLS90_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02188.1"
                     /translation="MTAPPTEPPRQPPPQSPWRIVARRLLRNRAALLGGGILLVFYFV
                     ALFAAPIAPYSPYATQSSVPDHPPHRLRFVDREGRFHWRPFVYRYELTDLLRRHYAMD
                     VGKRYPIRFFVRGDRYTMWWFIKSDWHLFGLEGGAADNVRLHLLGTDRTGRDIFSRII
                     HGSQISLSIGLIGIAITMTIGMLVGGLSGYFGGWIDSAMMRLTELIMSVPDLYLILAL
                     RAAFSSNNPLLLALLGMEPGQHLGSAQIYLLIIVILGLVGWGGTARVVRGQVLAVRDL
                     DYIQAARALGASRPRIILRHVLPNTFTYVIVAATLAVPGYILGEVALSFLGVGIEEPE
                     PSWGLMLQDGQNLRTLTQAPWVLAPGVFIFVTVFAFNFLGDGLRDALDPKAVDQA"
BASE COUNT         2338 a         5619 c         4616 g         2492 t
ORIGIN      
        1 cacgctgcgc accaggtgct ccagaatgcc gccggcgcgc accccctcgg gatcggcccc
       61 caccgcgtgg acgctgactt ccacgtcgat cagcttatcc atgtccaccc gcatcaagtc
      121 gatgtgcagg atgcggttgt tgaccggatg gcgctgcaca tcgcggatca gcgcctgctg
      181 ctgctcgccg ttgagctgga tgttgaacag cttgttcgtc cccgtcgccc cgtgcaggat
      241 catttcgatc gcgtggttgt cgatcgcgac catggccggc tccttgccgg cgccatagac
      301 gaccgcgggc acctgccctc cgcgccgtag tcggcggttg gcccgcttcg ggcccacctc
      361 gcgcggcttc agttcaagct gtagctgatc catggttctg ctccctgtgc tccggcccgt
      421 tacctatgca atgcgcgatt gcctgtggac ccgcgcgacc tgtggccgcg gcctcccgcg
      481 cgggaggtaa ctgggcgcac aaagactggt atcgtacgca ttgcgcgcgc cggattcaac
      541 aaaaaacggg cccttgtagc cgccgcgtca ttccggcggc cgggggctgg tccgcaaccc
      601 cgcgatcggc gcgccgtcgg gcgtcgtcag ccgcgcgatc gccccccagc cggcggtggc
      661 ctgcgtcacc ttcagcatca gcgcattcca gcccgcccgc agctcgaccg gcgcctcgtc
      721 cgcgcccgct tccagcccgc gcatcaccgg gtgctcgaac accggctcgc cattgagcca
      781 gaccttgatg ccgtcgtcgg agcccagctc cagccgggcc gccatcgccc gcggcgacca
      841 gacccacgtc cgcagatagg cgacccggtg gttgccgccg atcgcctcgc ccagcgacac
      901 cgcccccggc atccgccaca ccacggtcgg catgatccgc cacgccgcct cgtccgtccc
      961 cgcgggcgtc tcgggggcga acggttcggc cagcagttcc tcctggctcg cccccggctt
     1021 ctcgtagggg cccgccacct gccaggcggc cagcatcgga gtgagctgct cgatgaattc
     1081 gcggctcgcc tggatccgct cgcggatggc cgcgtcctcg ccggcggcct gctccacccg
     1141 gtcgatcagt tcgccggcca cctccggtcg cgcgccggca ttggcgcccg ccgcttgcag
     1201 gatcacttcg gccaactcgg ccttcaaccg gatctgaccc gcctcgaccc ggtccagcag
     1261 gttgtcggct tcgctggccg ccagttccgc atcgcgcccc gcgatcgcgc ccagcacctt
     1321 gatctcggcc gccagcgctt cgtcgcgcag ttgggcgtcg tccagacagg gccgaagaaa
     1381 ggcgagcgcc gccgggtcgg ccagctcggc cacgcccgcc agcacctggc ggcgttcctc
     1441 ataacgctcg gcggccgcca tcgcctgccc atacggctcc agccgccgcg cgctcaggtc
     1501 ggcgtcctgg ccttcatctt cattctcgtc gccgatctgc tcgatcaggc gaatgtagcc
     1561 gcgcagcgtc agcacccgct cgcgccgatc ctcggcctgc cgcgccagcc gcagcagatc
     1621 gggcatcgcc gccggcgacg gccactccgc cagcgcgcgc accgccgcct cgcgcacccg
     1681 gtcctgctcg gcgccggtcg ccccgcgcac ggcgcgcagc gccgattccc ctcccagccg
     1741 gcccagcacc ggcagcagac gcgcccgcag ttcggccgtc cgcgccgatt cgtatttttc
     1801 cagcaccggc cgcgccgccg cctccggatc gcccagccgc cgcgcgactt ccgccacggc
     1861 cgcgccggcc gccgcctgcc cgtccgggtc gatccgttcc agcaggtcca gcaggtcgtc
     1921 caggtgctct tcacggccca gttcgcccag cgcggccagc gccgccttgc gcacctccgc
     1981 gtcatcgctt tcggcctggg cgatcagttc cggcacggcc tgtgttgcgc cccgcgcgcg
     2041 taatgccgcg atcaccgcca gccgctcggc gttctccccc tgcgcgagca cttccaggaa
     2101 tgcgccatcg accccctcgc cggcgatccg gctcagcacc cggcgggccg tatcggcttc
     2161 ctcgccctcc ccgcgcgccg cccgcccgac cagccgcccg accgattcgg cgtcgccgcg
     2221 cgcgccgagc ccctccagcg cggcgatccg caccgcctcg tcatcgctct ccaggccgtg
     2281 ctcgagccac ggccgcgcgt cgatcgccgg gtcggcggcc gccgcccgca gcagcgcggc
     2341 ccgcgcgccg gccgggtatg catcgtaatc gtcgagcaag gcgcgcgtca ggtcactgcc
     2401 cgccagttcg cgcagaaacc ccgccgcttg cgcctgcatg gccgggtctc ctccgcgcag
     2461 gatttcgtca agctgcggcc gcgcatcgat gccgcgcgcc agcatcagac cgcgcaacgc
     2521 cgcgatgcgc cccgcttgcg tttcgggcgc ggcaagcagt cgttcataaa tcgccgctgc
     2581 gtccgcggcg gcgccttggc cgagcttgcg ctccgcgacg cgcagcagcg cggtctgcag
     2641 ctcgcggcgc ggttccggtc tcagcgtccg gattgggagt tccatcaatg cctcggccgc
     2701 cgcggcggaa tcgatcttcc ccagcgcggc gatcgcggcc gccgcgaccc ccgtcgaggc
     2761 gtcgtcggca agccgggtca gcggcccgac cgccatgacc tcgccgcggt cgccgaggct
     2821 gttgatcatc ccgatccgca attcgcccgc cgtctttttc atccccttga ggagggcccg
     2881 ggccgccgcg tcgccgggga tcgcctgcaa cgcgtggcgc gccatgtgac tcagttcggg
     2941 atcggccagc cggcgggcaa gctcgcgcac cgcgcggtcc gacccgatcc gccccagctc
     3001 gcgcgcggcg aagcgccggg catccggcgt cccggtcgcc agcagcgcga tcaggttgtc
     3061 ctcaagctgc cgattctgct cggcacggac gcggggcccc gccagcatct cctcgatttc
     3121 gctcaacgcc gcgcggcttt cgccgaattg gtaggcggcg gcccgttcaa acgcgtcgat
     3181 cgtctccgga gcgcccgtga atccgggtgc cggcccggcc agccacggcc gcgcgcatga
     3241 gaccccaagg ctcaggctca gaaccagaac gagcagtcgc accagctggc gtggtcccat
     3301 cgcggccttc cccctgcatg ttcccttcga tggttcggac ccaggacggg ccctgtccgt
     3361 cccgcccccg ccgctacagc cgccacggct cgcgcatcgc gcgcgagcag agccgctgcg
     3421 cttcctcatc gccgacgatg cgttcggcat cggggtccca tcggatcgtc cgtccggtca
     3481 acatcgcgat attgcccagg tgcgcgatcg tgatggagcg atgggcgact tcgatcggtg
     3541 cgatgggttc ttcgcgcgtc cgcacgctgc ggagaaaatc cgcgcggtga tcttcgcttt
     3601 gatagagctg tatttccccg ggtccgatcg gctcgtccag cagcgcggcc ggctcggcct
     3661 cgatcgcgcc ccgcgtcacg aatacccacc ccgcgtcccc ctcgaatcgc accccattgg
     3721 ggtatccgtc cgacacatcc atcgtgacgc cgtcgtcata ggtgcagatg aaccggtagt
     3781 gcgtgaacac gtcccacagt ccgtcgcgcg gacgctccca ggcgaccggc tcgaccttga
     3841 ctggcccggt cagctccgtc cccttgcccc attgcgcgat gtcgcagtga tgcgcgcccc
     3901 agtcggtcac ctgcccgccc gagtagtcga agatccagcg gaaatcccag tgacaccgcc
     3961 gttcgtgata gggcgcccac ggcgccggac ccagccagaa gtcataatca aaatgctccg
     4021 gcaccggctg cggctggact acttcgccga ccggcgcggt cggaagcccc acccgcatgt
     4081 tgtgcagccg gccgatcctc ccgttgcgca ccagttcgca ggcaaatcgg aacagccggt
     4141 cggagcgctg ctggctgccg gtctgaaaga cgcaaccgtg acgccgcacc tcatcgctca
     4201 tgatccgccc ctgctcgatc gtcagcgaca gcggcttctc gccgtagata tccttgcccg
     4261 cccgcgccgc atgcaccgtc gccagcgcgt gccagtgatc gggcagcgca atcacgacgg
     4321 cgtcgaggtc ctcgcgcgcc agcagctcgc ggtaatcatg ataaacgtgg cagcccgcgt
     4381 accgcccgct ccaggtccgc tcgccgtagt actcgttgac ggtgttcagc gcggcgcggg
     4441 cgcgctgggc atcgacgtcg cacagcgcga ccatctgcac cggcggcatg tgcaggaacg
     4501 ccgccatgtc ggcctcgccc atcgcgccca ggccgatggc gcccatcgtg atccgctcgc
     4561 cgggcggcgg cggcggcgcg gcgtggacgc gcccgatcga cgccagcgcg cccagtccgg
     4621 cggcggcgcc cagtgaacgc tggaggaact ggcgtcgatt gagcgatttc ggcggcatcg
     4681 cgatcttgct cgcatcgggg tgacgccagc ataggcactc gccccgcgaa tggaaagaac
     4741 gaaccgcgcg catcggccgc aacgatctag cctcacgcca ttttctgccg cttcctgccc
     4801 cctcgagtcg cttacgtccc gtttgtcctt ccgtcccgag gatgcgggac gagagacgat
     4861 tttttcataa cggggccgac atgaaatctt aagtgggcgc cccgttgatc cgatacgctc
     4921 atcagacggg ccgtcatggg cccccggcgg cctgggcggg ccgcccccct ccccgcggag
     4981 ttccctctga atgtcgcatt cgggtcacga tgcttccgac gcctcgccgt gggtgctggt
     5041 tgccgacgac gatcccacca tccggctctc gctcaagcac acgctcgaac gcgccggtct
     5101 gcgcgtcctg accgccgccg acggccgcga agccatcgag cagatgaccg aggaaatctc
     5161 cgccgcgatc ctcgatctga aaatgcccgg ccgcaacggc ctggaatgcc tgacccattt
     5221 ccagcagcac ttccccgacg tccaggtcgt catgatcacg gcgaccaacg acatccgcga
     5281 cgccgtcgag gcgatgaaac ttggggccta tgactacatc accaagccgt tcagccccga
     5341 cgagctgatc cggctgctgc gcaaggcggt cgcggccgcc gacctgcgac gcgaaaaccg
     5401 ccacctgcgc agcgccctgg gcgaaagcgt ctccagcacc accctgctcg gccagtcgct
     5461 cgccatgcgc cagctgcgcc agatgatcgg caaggtcagc cggctcgatt caaccgtgct
     5521 cctgctcggc gagagcggcg tcggcaagag cctggtcgcc cgctgcatcc accgccagag
     5581 cccgcgcgcc gccggtccgt tcgtcaccgt caactgcacg gccatgccgc gcgaactgat
     5641 ggagtccgaa ttgttcggtc atgaacgcgg cgcgttcacc ggcgcgctcg agcgccgcct
     5701 cgggcggctc gaaatggccc agcgcggcag cctgttcctc gacgaaatcg gcgacctccc
     5761 catcgacctc cagcccaaac tgctcagctt cctccaggaa cgcgtgttcc accgcgtcgg
     5821 cggcagccag gagatgcgct ccgatgtgcg gatcatcgcc gcgacgaatc atgatctgcg
     5881 cgacatggtc aacgccaaga tgtttcgcga ggatctctac taccggctca atgtcctgcc
     5941 gattacgatc ccgccgctgc gcgaccggcg cgaggatctg gaggagctga cgaccaacct
     6001 cctcgcgcgg atcgcccgca accgcggcgc ccggccctgc cgcctgagcg acggcgcgcg
     6061 ccgcgccctg ctcgcgcacg actggcccgg caatgtccgc gagctggaaa acgccctcga
     6121 gcgcgccacc gccttctgcg aaaacttcac cgtcatgccc gaggacttgc ccctcgagat
     6181 ccgggcccag ggccgccccg gcgccgacgg cccggccgcc gagccccagc actggctggg
     6241 cgggatgtcg ctcgacgacg tcgagcgcct cgcgatcgag cagacgctcg tccactgccg
     6301 cggcaacaag gccgaagccg cccgcaccct ccggatcagc gaaaaaagca tctataacaa
     6361 aatgaaacgc ctcggcctca acggccaccc ccgcccctcg ctttagcctc gtccctcgtc
     6421 tctcgtcctc gacttctctg ggaacgccac cctcccgact ggcttcgccc cgcttcaaaa
     6481 ctctccctca cagccctatc tttttcccag atcacaagac cccttgatct ttagatgatt
     6541 ccatctatca ttctctgtca acgacgtgat caccgccgtt ggtgaacgtt cgaagtcagg
     6601 agcagggtca tgcagggaac acgaaacgaa gaaacgggcg gcggccccac ggtccgtcgt
     6661 tttgtccagc cgatggcgct ggccgccatg atcgttatcc tggcgggact ggccatgacc
     6721 ggacacggcg acgatcccgc cgcaactcag caacaggagg catcgggatc catgagcggc
     6781 aggcaatcat ttttggggtt atcttcgatc gcgctgctgt cggacgccga aacacgttcg
     6841 atttcggccg aaaacccgac gggcgagaaa ggcggcggcg cccaggccga gcccggcagt
     6901 tacaacgtcg ccctcggcaa gggctggaaa tgccggcccg ccattacgct cgagcagcac
     6961 gccaccacca ccctggccga catccagggt cccggcacga tccagcacat ctggatcacg
     7021 gtcgacacga aagcctatcg cgactgcgtg ctgcgttttt actgggataa tgaggaaacg
     7081 ccgtcggtgg aagcccccct gggcgacttc ttcgccgtcg gccacggcct gcgttacaac
     7141 ctcaattcgc tcccggttgc ggtcaatccc aacggcgggt tcaactgtta ctggccgatg
     7201 ccgttccgcg agcgggcccg catcacgatc gagaaccagc gcgccgaggc gattcacagc
     7261 ttcttctatc agatcaccta cgagctggcg cccgtgccgc gcgaggcggc ttatttccat
     7321 gcgcagtggc gccgcaaggt ggtcgacctg gactatcccg aatacacgat cctcgacggc
     7381 gtcaagggcc agggccagta tgtcggcacg ttcatggccc tccagcagac cgccgatggc
     7441 tggtggggcg agggcgaaat caagttcttc atcgacggcg actcggaata ccccaccatc
     7501 tgcggcacgg gaaccgagga ttacttcggc ggcgcatggg gcttcggcga cacgttctcc
     7561 accccgttcc tcggctaccc gctctggtgg aaggagccgg gcgcggtgcc gcgccatgcc
     7621 ctctatcgct ggcatatccc cgacccgatc cggtttaagc aggatctgcg cgtcacgatc
     7681 cagaacctgg gcggctggcc cgatcgtccg gtcttcctgg cccgtttcga cgatatcgcc
     7741 tcgacggcct actggtatca gaccgagccg cacgcgccgt tccctgagtt cccgtcgctc
     7801 taccagcgct ggacgcgtaa ctggaacggc tgagccgcca tccgcccgct gatcggtatc
     7861 ggtcacggtg tgcgaccgcc gccctcgccg gtcactgtcc cggatcccgc gggatgcccc
     7921 ctcggtatcg gtatcgcaat cggtctcgat ctcgcctcag cacccccccg catccccagg
     7981 gatgccagac cgtagccggt ggttgagcgc agcgacacca caggtcaacg gccgctcaac
     8041 agcgccgcaa cccggaggga tgccagtaac gcaaaaaaga cgcaaacaag ctatgccggc
     8101 cagcggccgc aaaccccgct gcgtccagca gccctcgcgt cccttacgtc ccttatgtcc
     8161 tttcccccca gagggaggcc ccctcatgca cccggctcgg ccgccggctt gaacccgcgc
     8221 tgccgcaggt gctccagcac ccgctcgccg tggtaacgcg aattctcaat gaatatcgcc
     8281 ccgctgatgt tgccggccag catcacgccg atcatgtaaa tccccggccg cgggctctca
     8341 aacgtccggg ggtcaaacac cggctgcttg cgctcctcgt ccaccggcag ggtcagccgc
     8401 cccaggaact ccggatcggg gtggtatccg gtcagcgcca gcacggcatc gttctcgatc
     8461 tccagcgggc cctccgtctc gtgctggatc acgaccgaat cgggccggat ctcgcggaca
     8521 tgcgccggca tgatcgcccg gatccccccc tccttgatgc gattggccag atcgggggcg
     8581 atccagtatt tcaggccgct gaactcggcg cgccgatggc agatcgtcac ctcgaccccg
     8641 gcccgataca gctccagcgc agtctcggcc gccgagtgcc gcccgccgac gatcagcacc
     8701 ttcctcccga agtaatcatt cacttcggtg tagtaatggc tcaccttggg caggtcctcg
     8761 cccggcacat tcagcgcggc gggatgatcg taggcgccgg tcgccagcac gaccttctgg
     8821 caccgcgcct cgaacggaac gtcgccgccg gccacgccgc gcacgatgaa cctcccgtcc
     8881 tcgccctcca ccccccgcac ctcgtgccgc agttggaacg ccagcccgtg atcggtggcg
     8941 aagcgctgca ggtagcgcag atactggcgc cggtcgggct tctcatccgg gatcgcgagc
     9001 ggatagccgc ataattcgac cagatcagtc gtcgagaaca gcttcatgta gaccggccag
     9061 ttggccacgc cgtgcgcgaa acgcccgcgc tcgaggatca gcgtgtcgta gccggcgcgc
     9121 cccagcgtca gcgcggcggc cagccccgcc ggcccgctgc cgacaatcgc gatctgggtt
     9181 tcgtaagcaa gcatcatggg cgtcgatgga cggatttcgc ttccggaact gtccgctcga
     9241 tgccgacgat cccggaatac aggcccctcc accatacatg gagtggccgg cgccgttcaa
     9301 ggccgcaggc cggaaccctg cgtcgccgcc ccccacgacc aaacccctgc aaggggtggc
     9361 gggaaacgct tgaaatagag ggcctttcac gaccacttca gatggccgag gcctccccgg
     9421 acccgctcca cggccagtcc cgtcagcgac ggccgatttc agcccgttac gtcagtggcg
     9481 ggaaacgtga cgcaggaagc cgatggaggc ccgtcaggga cgacagaacc gctcgactgg
     9541 cacgaggacg atcacgaggc cgaagacgac gggaagagct gagatatcct gagattcaaa
     9601 accggcactt caccaaaacc cacatcgcct ggaaaaaatc gcgccacccg atcttctttc
     9661 cctcttccca cgtccgggcg gtgaagcgga ccggcagctc cacgatccgc tccccccggc
     9721 gcagcacctt ggccgtcacc tcggggcaga actcgaagcg ccggcaggtc agatcgatcg
     9781 cctgaatcac ctcgcgccgg aagattttgt aggccgtcgc ctcgtcggtg atccgctggc
     9841 cgtacagcag cgaaacggac cacgccagca ggtaattcgc gacgcgattg ggcaggcgca
     9901 tcttttcgat ccggcccagg aaccgcgagc cgtagaccac gctggcgcgg ccggccacga
     9961 tcggttcgag cagccgcggc aattcggccg gatcgtattc caggtccgcg tcctggatca
    10021 ggatgatctc gcccgcggcg tgcttcagcc ccgagcggat cgccatcccc ttgccgtggt
    10081 tgacctcgtg ccggacgagc accgtgtcgg gtcgcgaccc ctcttcctcg cacagcagtt
    10141 cccacgtccc gtcggtcgag tgatcgtcga cgaggatcat ctgtttgcgg aacggctgcg
    10201 cgcggacgcg gcgcagcacc tcgcgaatca gctcccgctc gttgtagatc ggcatgatga
    10261 ccgagacgag cggatcgttc agcggaggaa atggcggcgg cgcggcggga gttgggatca
    10321 tggaggcgtg gcttcccgcc gctgaaatcg acgcccggga cgcggaaccg ccccggcacg
    10381 gaacgctgac gatcttggcc cgcaaaacgc aggcgcgcaa gactgatctg cgccgcctgt
    10441 ggtagcatga aggcagcacc aggaagcggg ccgccggcgg acgcgcggcg gtccggccgc
    10501 ccggggagga gccccatcat gacatcgtcc gacctgcccc tggccgaacg gctcaagcgc
    10561 cagtcccacc gcatctgcac cgagatcctg cagtccgatg tcgagtgggt cgacatcgcg
    10621 attcagatcg agcggatgcg cgcgatgtgc gaggccgagg cccccgacaa actggagttg
    10681 ttcgaggcgc tctatgtggc gcgatttacg cgcctgtggc agcagtggcg cgagccggag
    10741 gagcgcgcgg cccgcccgcc gatcgccgcc aacccgatca tcgagggcga gtggcgcgag
    10801 acgcggggct gattcgcccg catcccgcct ctcgggcgcg cggcgggggc tgatcccagt
    10861 tgcccgcccg aaaagtagat gttattatcc tgaattatcc gcggcttgct ccgcgcgact
    10921 tcccgggggg gcgcctcgtg tccgaactgt tcctcaatca gcgccggctc gacatccagc
    10981 cgggcgccac gctcttcgac gccgccgatc tgcttggcct caagctgccc agctcctgcg
    11041 gccgccatgg ctcctgccat gaatgcatca tccaggtcac cgccggcgag gaagccctca
    11101 acccgcgcac cgagaacgag gatttcctgc gcgggggcta ccgcctggcc tgccagtgcc
    11161 ggctggagcg ccccgagccg gtcgtgcacg cgcatgcgct gcgccgcggc acgccgcaga
    11221 tcgccgccag cggccggccg gtccccgtcg cgctggcgcc ggcggtcacg cgcggcccgg
    11281 acgacaccgt cctgctcgac ggcgagccga tcgcccagcg gcgcggaccg ctgctcggcg
    11341 ccgccgccga catcggcacc accaccgtcg tcctgcgcct cgtcgatctg gagaccggcc
    11401 agatcgtcgc cgcgcagagc ttcgaaaatc cgcagatgtt cggcggcggc gacgtcatgg
    11461 gccgcatcac ctacgaccgc gaaaccaaac gccgcgatct gcagcaggtt ctggtggcct
    11521 acctcaatcg ctcgcttggc gagttctgcg aagccctcgg cgccgaaacc gacgacatct
    11581 atgagttcgt cgccgtcggc aacgccacga tgcgcgacct gctcttcggc tacgacgtcc
    11641 agacgatcgg cgtcaaaccg ttcatgtcgg tcagcgagat cgagttccgt gagggccagc
    11701 gcgagacgac ggcgctggcc tgccgcccca cccgcctgcg tctcgccatc aaccgcaagg
    11761 ggcgcgtctg gggggcgccg ctgatcgcct gccatgtcgg ggccgacacg gcggcgtgcg
    11821 tcgcggccgt cgacgtcgag cgcgccgccc gccccgtcat catcatggat atcggcacca
    11881 acaccgaact ggtcgtcggc cacggcgagc gcgtcctctg cgcttcctgt cccgccgggc
    11941 cggccttcga aggcgcgcgc gtcacctggg gcatgccggg cctcgaaggg gcgatcgaat
    12001 cggtccggct ggaaaacggc caggtcgact atcgcgtgat cggcgacgcg gcgccggccg
    12061 gcatctgcgg ctcaggcatg atcgacgccc tgggcgagct gctgcgtacc ggccggatga
    12121 acatgctcgg ccgcctggag gacggcgacg gccgcttcct catcgccgag gcggccggca
    12181 tctatctctc caataaggac atcagtgaac tggcccaggc caagggggcc aatatcgccg
    12241 gcatcctgat cctgctgcgc gagctcgggc tcacgctcga ggaggtcgac cgcttctatc
    12301 tggccggcgg attcgcccag tatatcgaca ttgaacacgc gatccgcatc ggcatgctcc
    12361 cgcggatgcc cgtggaaaaa ttcgagaagc tgggcaatgc cgcgatcgag ggcgccacgg
    12421 cgctcctctg ctcaaccgat ctgcgccgcc ggctcgacgc ttatgtccat cggatcggcc
    12481 atgtcgagct ggagaaccac cccgccttct tcggctatta cgtggaaggc atgcagttca
    12541 aagcctccga cgaaatggct cccgcgggcg tgtaaaagaa tccgtccctc gtcctcggct
    12601 ctcgaaaaac ccgccataag gggcacgaga acatcagggc cgcacaaacc ccggacgggc
    12661 cgcttgtcgc tggcgggagg cttggggata atgtcccggc cgcatgacgg ctgattcccc
    12721 ttgcgcgccg ccggcgtttg cccatgctgc gttttcttct ccgacgcctc tttcatggcc
    12781 tgttgatcct gctgggcgtc tccttcctga cgttctttct catcaatcag tcgccgggcg
    12841 acttcctcga ccgcaagcgg ctcgaccccc aggtcccgcg cgaattcatc gaggccgaaa
    12901 tccaacgcct tcatctggac cagccgtggc ccgtcgtcta ctgcttctgg ctcaaggaca
    12961 tcgtcacccg gctcgatttc ggccgatcct tcgaatacaa gcagccggtc ttcgatgtcc
    13021 tggccgggcg cgttctcaac acactcattc tctcgttctg ctcggtcgtc ttcgcctgga
    13081 tgctggcgat cccgctcggg atcatcgccg gttaccggca gtactcgctg gtcgataaga
    13141 ccgcctcggc ggtcgcgttc ctcggcgtct cagtccccaa cgtctttctc gccctgctcg
    13201 cgattttcct ggcggcgcgc accggctggt tccccaccgg cggcctcacc gatcagcgta
    13261 actggccgac gatgtccacc gccgagcgga tgctcgacat ggcccatcat ctgatcctgc
    13321 cgacgatcgt cctggggacc gggatgatgg ccgtgctgat gcgccagatg cgcggccaac
    13381 tgctcgacgt gctccaggcc gattacatcc gcaccgcccg cgccaagggc gtcggcgagc
    13441 gcgcgctgct gctcaagcac gccgtgcgca atgccatcaa cccgctgatc acgatcttcg
    13501 gcttctcgct ctcgacgctg ctctccggct cggtcctggt cgaaaaggtg atgagctacc
    13561 ccgggctggg gcgcctcacc gtcgacgcaa tgttcaacaa ggacatctat ctggtcatgg
    13621 cgtcggtcct ggtggccacc gcgatgctgg tgctgggcaa cctgatcgcc gatctgctgc
    13681 tggcctggag cgatccgcgg atcaagctgg aggatcgcgc gacgtcgtga ccgccccgcc
    13741 caccgagccc ccgcgccaac cgccgcccca gtcgccctgg cgcatcgtgg cgcgccgtct
    13801 gctgcgcaac cgcgccgcgc tcctcggcgg cggcatcctg ctcgtcttct atttcgtcgc
    13861 gctgttcgcc gcccccatcg ccccctacag cccctacgcc acgcagtcca gcgtgccgga
    13921 ccacccgccc catcgcctgc gcttcgtcga ccgcgagggg cggttccact ggcggccctt
    13981 tgtttatcgc tacgagctga ccgacctgct ccgccgccac tacgccatgg acgtcggcaa
    14041 acgctacccg atccggttct tcgtccgcgg cgaccgctat accatgtggt ggtttatcaa
    14101 gtcggactgg catttgttcg ggctggaggg gggcgccgcg gacaacgtcc ggcttcatct
    14161 gctcgggacc gaccgcaccg gccgcgacat cttctcgcgc atcatccacg gcagccagat
    14221 ctcgctcagc atcggcctca tcggcatcgc catcacgatg acgatcggca tgctggtcgg
    14281 cggcctctcg ggctatttcg gcggctggat cgacagcgcg atgatgcgcc tgaccgaact
    14341 gatcatgtcg gtccccgacc tctatctgat ccttgcgctg cgcgcggcgt tctccagcaa
    14401 caacccgttg ctgctggcgc tgctcggcat ggagcccggc cagcacctgg gctcggcgca
    14461 gatctacctc ctaatcatcg tgatcctggg gctggtcggc tggggcggca cggcccgcgt
    14521 cgtccgcggt caggtcctcg ccgtccgcga tctggactac atccaggcgg cgcgcgcgct
    14581 gggcgcctcg cggccgcgca tcatcctgcg ccacgtcctg cccaacacgt tcacctacgt
    14641 gatcgtcgcg gcgacgctcg ccgtgcccgg ctacatcctg ggcgaagtgg ccctcagctt
    14701 cctcggcgtc ggcatcgagg agccggaacc ctcctggggg ctgatgcttc aggatggcca
    14761 gaatctgcgc acgctgacgc aggcgccatg ggttctcgcg ccgggcgtct tcatctttgt
    14821 gaccgtcttt gccttcaatt tcctgggcga cggcctgcgc gacgcgcttg atcccaaggc
    14881 cgtcgatcag gcctgacgcc gagctccctc gcgcccgccc cgaacaaaat cggtcggatc
    14941 ggtcgaatcc gtcggatccg ccggatcgga acggcggacc cttacgaccc cgccaggtct
    15001 cgctggtgtt tgcggtagag cccgcggaac tgatggtagg tcaggccgag gcgctcggcc
    15061 gcccg
//
LOCUS       DAOPCO010000071        14975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4034_length_14975_cov_5.718298, whole genome shotgun sequence.
ACCESSION   DAOPCO010000071 DAOPCO010000000
VERSION     DAOPCO010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4034_length_14975_cov_5.718298"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLS90_06995"
     CDS             complement(<1..716)
                     /locus_tag="PLS90_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPK02189.1"
                     /translation="MLIEFVFWMALAILVGSFAIYLLFGLLNRLTPRLHRPLLRLIIQ
                     HLRAPLRLLGPLLAAKVVADSYPDLTIVAAVNHVIALLLIGAIAWLLIAAVRILRERI
                     MARYDITSPDNLAARKIATQLRVLQRILVILIVFIAAACALMTFDQVKAIGASLLASA
                     GIAGIILGFAAQKSLATFMAGLQIAISQPIRIDDVVIVEGEWGRIEEITLTYVVVRIW
                     DQRRLIVPITYFIEQPFQNWT"
     gene            complement(934..1311)
                     /locus_tag="PLS90_07000"
     CDS             complement(934..1311)
                     /locus_tag="PLS90_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HPK02190.1"
                     /translation="MGAGTLWGEPLFRRGVDEFNAGRYFAAHESLERLWLRTTGPRRE
                     FLAGLILLAGALHQLKVKRYPSAAARLFARANRRWHALPAVVDGLDLAAIESQCRRCL
                     TEEACPPRISLPGEHEAQEQLLE"
     gene            complement(1314..2105)
                     /gene="pgl"
                     /locus_tag="PLS90_07005"
     CDS             complement(1314..2105)
                     /gene="pgl"
                     /locus_tag="PLS90_07005"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPK02191.1"
                     /translation="MPEYANARVLIYDNRQEMIDAFCRHLVELGQDAIRRHGRFALAL
                     TGGRTPGPFYERLAEKPYRDQLEWSNVRIFFGDERCVPPDDDASNYKLARERLLSRVP
                     LSEKSIFRMPADDPDHARAARAYEAALERELNLNTLGIPAFDLVLLGVGEDGHIASIF
                     PGTEATRARDRHVMFNHVEKLGAWRMTLTYPVLNAARHVWILATDAGKREIAARALGH
                     LPGGNVLPIWHVRPADGRLVWWLSSEAAALIPPEEQDRPEGEDEV"
     gene            complement(2175..3206)
                     /locus_tag="PLS90_07010"
     CDS             complement(2175..3206)
                     /locus_tag="PLS90_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPK02192.1"
                     /translation="MPPELPPTNFLDPYFFTVHVVMLFMLGACFGSFFNVCIYRIPMN
                     VPLSLPPSHCYRCGRFIRWYDNIPLISYWVLGGKCRHCGASFSIRYFCVELLTALLFL
                     AAFLKLGYTLALLPALVFLSLLIIATFTDIDHWIIPDRVSLGGMAAGLVLAAIPPVAW
                     ARGNPLAVHLFPVAEPLAPLANAAVGAAVGYGGLWLVGKLGTLLFRKDAMGVGDMKLM
                     AMFGAFLGPEPLLHVLILACLVGTAAGLAGLARARLTRDRVVDPATAPLALSGEEVDA
                     LLARMPLDAAEARVVDAALRKPGVLGPVRHHLPFGPSLAVAAAIVYFYFPQLRALLER
                     LVLPGPLVP"
     gene            complement(3216..3821)
                     /locus_tag="PLS90_07015"
     CDS             complement(3216..3821)
                     /locus_tag="PLS90_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008674361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02193.1"
                     /translation="MDHLTRLTQRYGPAIAPLIADERLNPLDGGEPHGAVLGALERLT
                     VDSAFAGFNIQDRAMAAAVISGLWLYHNYLERSHAISQSIANATGSCWHAIMHRREPD
                     AANSAYWFRRVGEHPIFPELRAAARAEAAGAPLAPHAAFLLDQRHWNPFAFIDLCEAC
                     RGSGDATEALCRRIQLREWKLLFEYSFDQAVVGGRPLAGEA"
     gene            complement(3886..4248)
                     /locus_tag="PLS90_07020"
     CDS             complement(3886..4248)
                     /locus_tag="PLS90_07020"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPK02194.1"
                     /translation="MRSGMGIPAAALCVGLLLATACSIIENEGKIGVVEQVQQASLDE
                     TYQATLKALGDLEFDVTRSDVDKLTGYVEGTTADQKRFEIILEKLTDEATNVRIHFEP
                     MGDKPRSALVQQEIAKNL"
     gene            complement(4367..4867)
                     /gene="lepB"
                     /locus_tag="PLS90_07025"
     CDS             complement(4367..4867)
                     /gene="lepB"
                     /locus_tag="PLS90_07025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPK02195.1"
                     /translation="MTRNSPKSPLTRLRVAVATVILGILFGLLYLVQTRKVAFFEVTS
                     QSMAPTLQEGERFLMFTPSQYRVGDIVVFSLPETPETHTVKRIIAEGPATVEVRDGLI
                     RVNGRPAPPPQGDAPPIPGLNADWTVQPGEVFLAGDNRDDSYDSRDYGPVPIERLQGI
                     LWLHPF"
     gene            complement(4926..5834)
                     /locus_tag="PLS90_07030"
     CDS             complement(4926..5834)
                     /locus_tag="PLS90_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02196.1"
                     /translation="MIAPANGFKRNLSRAGHRAARRWPVLAFAMLLAAATAAAQPDAG
                     RVEAAVTPLVPPALTPATADVPSTAGPALAPPDLAAIPEIVPPLSVNESRRMILEVVE
                     LLEEQPDLRDRPQLLELQDRKLRAASRYIERIDRAFSTLPYYHWELVSDEKIPGGEGG
                     EQLITLREPLPGVTALSLRVNNQDVMIDSMEVIDVSGKVWEFNNPILLPAFRPRHEVC
                     FLPLPTDLKQLRITSRTANPRPDQRRPRLTIHAGVCTLPESGKHALHHIHEARERIRN
                     GESAAAAAQLRRAALLLQEFVNSRRL"
     gene            6273..7697
                     /locus_tag="PLS90_07035"
     CDS             6273..7697
                     /locus_tag="PLS90_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK02197.1"
                     /translation="MLGQPCQRVFCIVPPTGRYIREDRCQTPIEKMKTIALRPPIDLM
                     YAAGSFEAAGAEVRLTDYPGEQWGWDRLEQDLREFQPDAILLSITTPSLDQDMEAAVL
                     AKRVDPKIVTMAKGAHFNTRDVQTLEQYAALDVVFRGEYEQTCAELARGAAPASILGI
                     TWRDTDGAVQRNGDRPFTDDLDAIPFPARHLTHNELYIRPDTMEVQTTIITNRGCPFH
                     CIYCLANQVSGTKNRYRSVENVVAEIRQCVERHGIRNFLFRSDLFTQNKKWVIELCQS
                     IIEAGLDIQWASNSRVDTVNPEVLGWMRKAGCWIIALGVESGDQETLDRIQKKATVAQ
                     AFQAVRWIREAGIKSSVYLLMGLPWDTPETIERQMAFARELDPDILEIFYPYPFPGTA
                     LYDLCVKQGLIREGEIPRDAYSDPATGGLYMSVDDFKRLRKQAIRDFYLRPSKIIRTL
                     RGAPTLGEKINYIKAGVRELFSHA"
     gene            complement(7745..9055)
                     /locus_tag="PLS90_07040"
     CDS             complement(7745..9055)
                     /locus_tag="PLS90_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK02198.1"
                     /translation="MWRFRLTRNDPTLAFRGGWRHWVRFAVLLAALGLATLALILAGE
                     ELWGPAWAEPARTWLYRVAFWIVLPVRGLVLPLAREVNHHWPARHYMLLGLALPLWFG
                     VLGWIAHRLGQRARVKLGRGGEAASARTAVEPAPAATVAQPGRRRFLIGAALGVGGGA
                     AAGAGVYGVWLLPSMLRVRHYDIPVPDLPAALDVLRVVHLSDTHYGPFISLSYLKEVI
                     ERINALRPDLVLLTGDYVHRTENSVEPGIAALAALRARLGVAAVLGNHDHWEGAPAVR
                     RAFAAADIPLVDNDRLFLTVDGLAREPADPADALCVAGVGDLWEDEVEPEQALAGVPA
                     ATPRLLLSHNPDVAEHLNPRLRVDLMLCGHTHGGQVYLPLIGAPIIPSAYGQKYVGGM
                     CRGPTCPVLVTRGVGMAFLPVRLGVPPEFGVLTLRRTGSAAART"
     gene            9168..10652
                     /locus_tag="PLS90_07045"
     CDS             9168..10652
                     /locus_tag="PLS90_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPK02199.1"
                     /translation="MDRAAPPIATEPPVMDSDLVVLLSRIQFAVTIGFHYLYPPLSIG
                     LGLILVFMEGMFLRTGNVIYETMTRFWVRIFGLTFAMGVASGIVMEFQFGTNWSAYSR
                     FVGDIFGAALAAEGIFAFFLESGFLAILLFGWDKVSPRMHFLSTVLVCLGAHFSAVWI
                     IVANSWQQTPAGYHLVENAGAPRAELISFWEAVLNPSTLARLTHVLLGAYILAGFFVM
                     SITAFYLLRGRHQRFARRALVIGLLVAAFASLAQLVVGDWSARVVARHQPLKLAAFDA
                     IYKTEPRTPMFLFGFPDDERQTVRYGLPIPGLMSLLLHLDMETPVTGLDSRPRDEWPP
                     VALSFQLYHVMIALGLFFVAVTLAGLFHLWRGTLFQQRWYLKLLVPAVVLPYIANQVG
                     WGAAEVGRQPWIVYGLLRTRDAVSPAVKPYEVWVSLVLFTAIYLALFFVFLYVLDRKI
                     RQGPEPLKPDDLAGGPHPPEDFLDAISRRGKMQFHATEEAQEGA"
     gene            10654..11670
                     /gene="cydB"
                     /locus_tag="PLS90_07050"
     CDS             10654..11670
                     /gene="cydB"
                     /locus_tag="PLS90_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPK02200.1"
                     /translation="MILNEIWFLLVGVLLIGYAILDGFDLGVGALHLFSRGDTERRLL
                     INAIGPVWDGNEVWLVTAGGALFAAFPHAYATAFSAFYLPFILLLFMLIFRALAIEFR
                     GKEPMGWWRRMWDVSFCVASIVASVLFGVAIGNMALGIPVGPDMEYQGNLLTLLGPYP
                     LLVGVFNLSIFTMHGAIYLYMKTEGDLQRKVKGWIVRATAFFIILYVLTTVYTLAAVP
                     SMIANFRDYPWIWGLVVLNVLAIANIPRAIYHEWPFRAFLSSCAAIAALISLFGMGVY
                     PNLIRSTLGDAFNLTIVNAASSQKTLGIMLLIAVIGIPFVLAYTAAIYWIFRGKVRLG
                     EHSY"
     gene            12072..13508
                     /gene="cysS"
                     /locus_tag="PLS90_07055"
     CDS             12072..13508
                     /gene="cysS"
                     /locus_tag="PLS90_07055"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPK02201.1"
                     /translation="MPLLIFNTQTRRKEPFEPLDPPRVTIYNCGPTVYDHFHIGNARN
                     FVVMDLVRRYLQYRGYQPFFVQNLTDIDDKIISRAQQEGVACEDITGRYIPSYFADAA
                     KLRVRMADAHPRATDYVADIIGLIERLIARGLAYAANGSVYFRVKAFREYGKLSGRRV
                     EDLLEGARVEVSDEKEDAADFALWKAAKPGEPTWDSPWGPGRPGWHIECSCMAMKLLG
                     ETIDIHAGGTDLMFPHHENELAQSEGATGRPFARYWMHNGFLNIDSQKMSKSLGNFLK
                     IDQVLQRASAPAVRHFLLSAHYRNPLDLTDEALERSAAAVRRINDGIETAAKLLALER
                     AEAAAGDVAAYRAEFEAAMDDDFNTPRALALLFKLVTEIHQLRADAVHAAGEAKADVL
                     GRLAALVEFARELRAFFSLEPEEEETDHASADSLTESLMELLIETRAAARKARAFAIA
                     DRIRDRLGELGIALEDHPQGTIWKKKES"
     gene            13620..14393
                     /locus_tag="PLS90_07060"
     CDS             13620..14393
                     /locus_tag="PLS90_07060"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPK02202.1"
                     /translation="MAPVPDMQYAEVRARCGACIHPARSAGQADLYELDWEGRPALLK
                     DFSQRPRLVRWLFSRRIAAREVRMLRRLEGLEGTPRLLATAGPEAFVMERLPGERIPN
                     RKRGGPPAIFWANARALLDQLHARGVAHGDLRRKNILAGPNGEAYLIDFATAIRRDTP
                     GGGRGSWLGGYLFKRCRTIDRNSFARIKAQYAPEALDATERRWLEEMPLYLRVGRMLK
                     KRVYRLRKRRYWRKRVKWVKWLRRKLRARKRQGRRGSRG"
     gene            14556..>14975
                     /locus_tag="PLS90_07065"
     CDS             14556..>14975
                     /locus_tag="PLS90_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02203.1"
                     /translation="MRQLTPWLLLLAMSSGLAGCYSLRHTTASGKPVMADTALAENQE
                     ITQHFKETARSVYLLDGLIPLDRDDAQALLYEYVNAGDGIVNLRIREQFTFIDSLIGW
                     VTLGIVTTRTVRVEGDVFINRTVASAAPVLIEVDAEHP"
BASE COUNT         2561 a         4912 c         5086 g         2416 t
ORIGIN      
        1 gtccagttct ggaacggctg ttcgatgaag taggtgatcg ggacgatcag ccggcgctgg
       61 tcccagatgc ggacgacgac gtaggtcagc gtgatttcct cgatgcggcc ccattcgccc
      121 tcgacgatga cgacatcgtc gatgcggatc ggctggctga tggcaatctg gagaccggcc
      181 atgaaagtgg cgagtgactt ctgcgccgcg aagcccagga tgatgccggc gatgccggcc
      241 gaggcgagca ggctggcgcc gatcgccttg acctggtcga acgtcatcag ggcgcaggcg
      301 gcggcgatga aaacgatcag gatcacgagg atccgctgca ggacgcgcag ctgcgtggcg
      361 atcttgcgcg ccgccaggtt gtcgggcgaa gtgatgtcgt agcgcgccat gatccgctcg
      421 cgcaggatgc gcacggcggc aatcagcagc caggcgatgg cgccgatcag cagcagcgcg
      481 atgacatggt tgaccgccgc gacaatcgtc aggtcggggt acgagtcggc gacgaccttc
      541 gcggccagca gcggccccag caaccgcagg ggggcgcgca gatgctggat aatcaggcgc
      601 agcagcgggc ggtgaaggcg gggggtgagc cggttgagca ggccaaagag gagatagatc
      661 gcgaacgacc cgacaaggat cgccagggcc atccagaaaa caaattcgat cagcatcgga
      721 aacctcctgg tcggaaaatc aagggaccag tcccttaagg aagaccgcgg ggatccttcc
      781 cgcgcgatag gggatacatc acgttatcgg tccgcgcgcc gattggcaac ccgtggccag
      841 ccggcgtgcg atgcgggtaa ggatgtttgc cagcaaaaaa tccgtcggat cggacggagc
      901 cgacggattt caaccgcatc ccaatttcgt cgatcactcc aaaagctgtt cctgggcctc
      961 gtgctcgccg ggcaagctga tccgcggggg gcaggcctcc tcggtcaggc agcggcggca
     1021 ctggctttcg atcgccgcga ggtccagtcc gtcgaccacc gccggcagcg cgtgccagcg
     1081 gcgattggcc cgggcgaaca gacgcgccgc cgccgaaggg tagcgcttca ctttgagttg
     1141 atgcagcgcg ccggcaagca ggatcaggcc ggcgaggaat tcgcgccgcg ggccggtggt
     1201 gcgcagccag agccgttcga gcgattcatg cgccgcgaag tagcgccccg cgttgaactc
     1261 atcgactccc cggcggaaga ggggctcgcc ccacagcgtg ccggcgccca tgctcagacc
     1321 tcgtcctcgc cctcggggcg atcctgctcc tcgggcggga tcagcgcggc ggcctcgctg
     1381 ctcagccacc agacgagccg cccgtcggcc ggccgcacgt gccagatcgg caggacgttg
     1441 ccgccgggca gatggccgag cgcacgggcg gcgatttccc gcttgccggc gtcggtcgca
     1501 aggatccaga cgtggcgcgc cgcgttcagc accggatagg tcagcgtcat gcgccaggcg
     1561 ccaagcttct cgacgtggtt gaacatcacg tggcgatcgc gcgcgcgcgt cgcttcggtg
     1621 ccggggaaga tcgaagcgat gtggccgtcc tcgccgaccc ccagcagcac gagatcgaag
     1681 gcgggaatcc ccagcgtgtt gagattcaat tcgcgttcga gcgccgcctc gtacgcccgc
     1741 gccgcacgcg cgtgatccgg gtcgtcggcc ggcatgcgga agatgctttt ctccgacagc
     1801 ggcacccggg agagcaggcg ttcgcgcgcc agcttgtagt tggaggcatc gtcgtcgggt
     1861 ggaacgcagc gttcgtcgcc gaaaaagatg cgcacattgc tccattcgag ctgatcgcgg
     1921 tagggcttct cggccaggcg ttcgtagaac ggtccgggcg tccggccgcc ggtcagcgcg
     1981 agcgcgaagc gtccgtgccg gcggatggcg tcctggccca gctcaaccag gtgtcgacag
     2041 aaggcgtcga tcatctcttg gcggttgtcg taaatcagga cgcgggcatt ggcatattcg
     2101 ggcatgaaac ggattccttg gatgaagttg aggacaagtg atccggggag cgaaaaatgt
     2161 gcccccggcg gcggtcacgg caccagcggg ccgggcagga cgagccgctc gagcagggcg
     2221 cgcagctgcg gaaagtagaa gtagacgatg gcggccgcga ccgccagcga aggcccgaac
     2281 ggcagatgat gacgcacggg gccgaggacg ccgggtttgc gcagcgcggc atccacgacg
     2341 cgcgcttcgg ctgcgtccag cggcatccgc gccagcaacg catcgacctc ctcgcccgaa
     2401 agtgccagcg gcgccgtggc cgggtcgacc acccggtcgc gggtcagccg cgcgcgcgcc
     2461 aggccggcca gccccgccgc cgtgccgacc aggcacgcca ggatcagcac gtggagcagc
     2521 ggctcggggc cgaggaacgc gccgaacatc gccatcagtt tcatgtcgcc cacccccatc
     2581 gcatccttgc ggaaaaggag cgtgccgagc ttgccgacga gccagagccc gccgtagccg
     2641 acggcggcgc cgacggcggc attggccagc ggggccagtg gttccgccac cgggaagaga
     2701 tgcaccgcga gcgggttgcc ccgggcccag gcgactggcg ggatcgccgc cagcaccagc
     2761 cccgccgcca tcccccccag gctgacgcgg tcggggatga tccagtggtc gatgtcggtg
     2821 aacgtggcga tgatcagcag cgacaggaag accagggccg gcaggagcgc gagtgtgtaa
     2881 ccgagcttga ggaacgccgc caggaaaagg agcgccgtca gcagctcgac gcagaaataa
     2941 cgaatggaaa agctcgcgcc gcaatggcgg cattttcccc ccagcaccca gtaactgatc
     3001 agggggatgt tgtcgtacca gcggatgaac cggccgcagc gatagcagtg cgacgggggc
     3061 aggctcagcg ggacattcat cggaatccga tagatgcaga cgttgaagaa cgagccgaag
     3121 cacgccccca gcatgaagag catcacgaca tggacagtga aaaagtatgg gtcgaggaaa
     3181 ttggtggggg gcagctcggg aggcatggtg acgcgctacg cctcgcccgc gagcggccga
     3241 ccgccgacga cggcctgatc gaatgaatac tcgaacagca gtttccattc gcggagctgg
     3301 atgcgccggc agagcgcttc ggtcgcatcg ccgctgcccc ggcatgcttc gcacaggtcg
     3361 atgaaggcga acggattcca gtggcgctga tcgaggagaa acgcggcatg gggggcgagg
     3421 ggcgcgccgg cggcctcggc gcgcgccgcc gcgcgcaact ccgggaagat cggatgctcg
     3481 ccgacgcggc ggaaccagta ggcgctgttg gccgcgtcgg gctcgcgccg gtgcatgatc
     3541 gcgtgccagc aactccccgt tgcattcgcg atcgactggc tgatcgcatg cgaacgctcg
     3601 aggtaattgt gatagagcca caggccgctg atcacggcgg ccgccatcgc gcggtcctgg
     3661 atgttgaaac cggcaaacgc cgagtcgact gtcaggcgtt ccagggcgcc cagcacggcg
     3721 ccatggggtt cgccgccgtc gagcggattg agccgctcgt cggcgatgag cggagcgatc
     3781 gccggaccat agcgctgcgt cagccgtgtc agatggtcca tcccgaacta ccttgccggt
     3841 ctcgccgtca gcaaaccgcc ggttggaact gcatggcggg cgcgctcaca ggtttttggc
     3901 aatctcctgc tgaaccagcg ccgagcgcgg cttgtccccc atcggctcga agtggatccg
     3961 cacgttggtc gcttcgtcgg tcaatttttc caggatgatc tcgaaacgct tctggtcggc
     4021 ggtcgttccc tcgacatacc cggtcagttt gtcgacatcg ctgcgggtga cgtcgaattc
     4081 gaggtcgccc agcgccttca gcgtcgcctg ataggtctcg tccagcgagg cctgctgcac
     4141 ttgttcgaca acgccgatct tgccttcgtt ttcaatgatc gaacacgccg tggccagcag
     4201 caggccgacg caaagggccg ccgccggaat tcccattcca gatcgcatag ccgttggtcc
     4261 tttcccaggg tggatgagtc aataaaatcg tgccctactt tcggcgaacg gcctcccgca
     4321 tgcaagtcaa caaatacgtc cccggtgcgg tcgatcgtcc gctgtcttaa aacggatgca
     4381 gccagaggat cccttgcagg cgctcgatcg gcacggggcc gtaatcgcgc gaatcatagc
     4441 tgtcgtcgcg attgtcgccg gccagaaaga cttcgccggg ctggaccgtc cagtcggcgt
     4501 tcagccccgg gatcggcggg gcgtcgccct ggggcggcgg ggcgggccgg ccgttgaccc
     4561 ggatgagtcc gtcgcggacc tccaccgtgg cggggccctc ggcgatgatc cgcttgaccg
     4621 tgtgcgtctc gggcgtttcg gggagcgaaa aaactacgat atcgccgacg cgatactggc
     4681 tcggcgtgaa catcaggaag cgctcgccct cctggagcgt cggcgccatc gattgggacg
     4741 tgacttcgaa aaacgcgacc ttgcgggtct ggacgaggta gagcaggccg aagaggatgc
     4801 ccaggatgac ggtcgccacg gcgactcgca ggcgcgtcag cggcgatttt ggggagttcc
     4861 gcgtcatggg gcgagtgctg actccgggga gcggggtcgg tccgcagacg gagcaagcca
     4921 gccggctaca gtcggcggct gttgacgaac tcctgcaaca gcagggcggc acgccggagc
     4981 tgggcggcgg ccgccgccga ctcgccattg cggatccgct cgcgcgcctc gtggatgtga
     5041 tgcagggcgt gcttgcccga ctcagggagg gtgcagaccc ccgcatgaat cgtcaggcgc
     5101 ggccggcgct gatcgggtcg tggattggcc gtccggctgg tgatgcgcaa ctgcttcaga
     5161 tcggtcggca gcggcagaaa gcagacctcg tgacgcgggc ggaacgccgg caacaggatc
     5221 gggttgttga attcccagac cttgcccgac acatcgatga cttccatcga atcgatcatg
     5281 acatcctgat tattgacgcg caggctgagc gcggtcacgc cgggcaacgg ctcgcgcagc
     5341 gtaatcagtt gctcgccccc ctcgccgccg gggatttttt cgtccgacac cagttcccaa
     5401 tgataatacg gcagcgtgga gaacgcgcgg tcgatccgtt cgatgtagcg cgaggccgcc
     5461 cgcagcttgc ggtcctgcaa ctcaagcagt tgcggtcggt cgcgcagatc gggttgctcc
     5521 tcgaggagct cgaccacttc gaggatcatc cggcgcgact cgttcaccga caggggcggg
     5581 acgatctccg gaatcgccgc caggtccggc ggcgccaggg ccggaccggc ggtcgagggc
     5641 acgtcggccg tcgccggcgt caatgccggc ggaacgagcg gtgtgacggc ggcttcgacg
     5701 cggccggcgt cgggctgcgc cgcggccgtg gcggcggcga gcagcatcgc gaaggcaagc
     5761 accggccacc gccgcgcggc gcgatgcccc gcccgggaga ggttgcgctt gaaaccattt
     5821 gccggtgcga tcatcggccc agcccgccgg ttcctggccc ggagattgcg cgccacaaga
     5881 ttcacccagt acagcttaac ccgccgtggg atgatgcgca atgcccggct ccaccgggcc
     5941 aaagttcgtc aggttgcgat cgtgttgaat ttcaaaaggg acacagggga cgtgaaggac
     6001 ataagagaaa tggagaggcc ggcttggagc tttcagagga cagccgtcgt cactcgcgaa
     6061 gataaccctc gccatccgcc ccttccgtcg cttccgtccg ttatgtccct tatgtccctt
     6121 ctgcaaacgc acgctcggca gcttgacgga ttcaccgggg gtctttaaac tgaaagggcg
     6181 cgtctggcgt tgccgcggaa cgagcgggga aggcgcggcc gcggacgcgg gtcagtcgcg
     6241 caagtcatcc gttttcagac agtcagcctc ccatgctggg ccaaccctgc caacgcgtgt
     6301 tctgcatcgt gccccccacc gggcggtaca tccgggagga tcgttgccag acgccgatcg
     6361 agaagatgaa gacgatcgcg ctgcggccgc cgatcgacct gatgtatgcg gcggggagtt
     6421 tcgaggcggc cggcgccgag gtgcgcctga ccgactaccc cggcgagcaa tggggctggg
     6481 accggctgga gcaggacctg cgcgaattcc agcccgacgc catcctgctg agcatcacga
     6541 caccctcgct ggaccaggac atggaggcgg ccgtgctggc caagcgcgtc gacccgaaga
     6601 tcgtgacgat ggccaagggc gcgcatttca acacgcgcga cgtccagacg ctggagcagt
     6661 acgcggcgct ggatgtggtc ttccgcggtg agtacgaaca gacgtgcgcc gagctggcgc
     6721 gcggcgcggc cccagcctcg atccttggga tcacctggcg cgacaccgac ggcgccgtcc
     6781 agcgcaacgg cgaccggccg ttcaccgacg acctggacgc gatcccgttc ccggcgcgcc
     6841 acctgaccca caacgagctc tacatccgcc ccgacacgat ggaagtgcag acgacgatca
     6901 tcaccaatcg cggctgcccg ttccactgca tctactgtct ggccaatcag gtatcgggca
     6961 cgaagaaccg ctatcgttcg gtcgaaaacg tcgtggccga aatccgccag tgcgtggagc
     7021 gccacggcat ccgcaatttc cttttccgtt ccgacctgtt cacgcagaac aagaagtggg
     7081 tgatcgagct gtgccagtcg atcatcgagg cggggctcga catccagtgg gcgagcaaca
     7141 gccgggtcga tacggtcaac cccgaggtgc tcggctggat gcgcaaggcg ggctgctgga
     7201 tcatcgcgct gggcgtggaa agcggcgacc aggaaacgct cgaccggatc cagaagaagg
     7261 cgacggtggc gcaggcgttc caggccgtgc gctggatccg cgaggcgggg atcaagtcaa
     7321 gcgtctacct gctgatgggc ctgccgtggg acacgcccga aacgatcgag cggcagatgg
     7381 cgttcgcccg cgagctggat cccgatattc tggagatctt ctatccttat cccttccccg
     7441 gcacggcgct ctacgacctg tgcgtcaagc aggggctgat ccgcgagggg gagatcccgc
     7501 gcgacgccta cagcgatccg gccaccggcg ggctttacat gtcggtcgac gatttcaagc
     7561 gcttgcgcaa gcaggcgatc cgcgatttct acctgcggcc gtccaagatc atccggacgc
     7621 tgcgcggcgc gccgacgctg ggcgagaaaa tcaactacat caaggcgggc gtccgcgagc
     7681 tgttcagcca cgcctgaagc gaacgcggct tgcagaatcc gccggatcgg ccggcgccga
     7741 cgatttacgt ccgcgccgcg gccgaaccgg tgcggcgcag cgtcagcacg ccgaattcgg
     7801 gcggcacgcc cagccgcacc ggcagaaacg ccatccccac gccgcgcgtc accagcaccg
     7861 ggcaggtcgg gccccggcac atgccgccga cgtacttctg gccgtaggcc gaggggatga
     7921 tcggcgcgcc gatcagcggc aggtagacct gcccgccgtg cgtgtggccg cacagcatca
     7981 ggtcgacccg gagccggggg ttgaggtgct cggccacgtc ggggttgtgc gacagcagaa
     8041 gccgcggcgt cgccgccggc acgccggcca gcgcctgctc gggctcgact tcgtcctccc
     8101 acagatcgcc gacacccgcc acgcacagcg cgtcggcggg gtcggccggt tcccgcgcga
     8161 ggccgtcgac cgtcaggaac agccggtcgt tatccacaag gggaatgtcg gcggcggcaa
     8221 aggcccgccg caccgccggc gccccttccc agtgatcgtg gttgcccagc acggcggcca
     8281 cgcccagccg cgcccgcaag gccgccagcg ccgcgatccc cggctcgacc gaattttcag
     8341 tccggtggac atagtcgccg gtcagcagga ccagatcggg ccgcagcgca ttgatccgct
     8401 cgatgacctc cttgagataa ctgagcgaga tgaacgggcc gtagtgcgta tcggagagat
     8461 ggaccacgcg caagacgtcg agcgccgccg gcaggtcggg tacggggatg tcgtagtggc
     8521 gcacgcgcag catgctgggg agcagccaga cgccgtagac gccggcgccg gcggcggcgc
     8581 cgccgcccac gcccagcgcc gcgccgatca gaaaccggcg ccggccgggt tgcgcgacgg
     8641 ttgcggcggg cgctggttca accgcggtcc gggccgaggc ggcctcgccg ccgcgtccta
     8701 atttcacgcg cgcgcgctga ccgagccgat gcgcgatcca ccccagcacc ccgaaccata
     8761 gcggcagagc cagccccagc agcatgtagt ggcgcgccgg ccaatgatga ttgacctcgc
     8821 gggccagcgg cagaacgagg ccgcgcaccg gcagcacgat ccagaacgcg acgcgataga
     8881 gccaggtgcg ggcgggctcg gcccaggcgg ggccccagag ctcctcgccc gccaggataa
     8941 gggcgagcgt cgccaggccc agcgccgcca gcagcacggc gaagcgcacc cagtggcgcc
     9001 agccgccgcg aaaggccagg gtcgggtcgt tgcgggtcag tcgaaaccgc cacatcgtga
     9061 ccgcgtcaaa tgcaaggacg gggccagcgg gacgccgatc attgcagata aacgaatccc
     9121 tatttccctt gttttctccg cgccctttcc ctatacgtac gcaggggatg gatcgcgccg
     9181 ccccgcccat cgccaccgag ccgcccgtca tggattccga tctggtcgtt ctcctctcgc
     9241 gcatccagtt cgccgtcacc atcggcttcc actacctcta tcctccgctc agcattggcc
     9301 tggggctgat tctcgtcttc atggaaggga tgtttctgcg cacggggaac gtgatttacg
     9361 aaacgatgac ccggttctgg gtgcggatct tcgggctgac gttcgccatg ggggtcgctt
     9421 cggggatcgt gatggaattc cagttcggca cgaactggtc ggcctactcg cgattcgtcg
     9481 gcgacatctt cggcgcggcg ctcgccgctg aggggatctt cgccttcttt ctggagtccg
     9541 gtttcctcgc catcctgctc ttcggctggg acaaggtgtc gccgcggatg cactttttgt
     9601 ccaccgtgct ggtctgcctc ggcgcgcact tctccgccgt ctggatcatc gtcgccaaca
     9661 gttggcagca gacgccggcc ggttaccacc tggtggaaaa cgccggcgcg ccgcgcgccg
     9721 agctgatcag tttctgggag gcagtgctca acccctccac gctggcgcgg ctgacccacg
     9781 tcctgctcgg cgcctacatc ctggccggct tcttcgtgat gagcatcacg gcgttctatc
     9841 tgctccgcgg gcggcaccag cggttcgccc gacgggcgct ggtcatcggc ctgctcgtcg
     9901 ccgcgttcgc ctcgctggcg caactggtcg tcggcgactg gtcggcgcgc gtcgtcgccc
     9961 ggcaccagcc gctcaagctg gccgccttcg atgccattta caagaccgaa ccgcgcacgc
    10021 cgatgttcct gttcgggttc cccgacgatg agcggcagac ggtgcgctac ggtctgccga
    10081 tccccggcct gatgagcctg ctcctccacc tcgacatgga aacgccggtc acggggctgg
    10141 acagccggcc gcgcgacgag tggccccccg tcgccctgtc gttccagctg taccacgtca
    10201 tgatcgcgct ggggctgttc ttcgtcgccg tcacgctggc gggcctcttc catctgtggc
    10261 gcggcacgct gtttcagcag cggtggtatc tgaagctgct cgtgccggcg gtcgtcctcc
    10321 cttacatcgc gaaccaggtc ggctggggcg cggcggaagt cggccgccaa ccgtggatcg
    10381 tttacggcct gctgcgcacg cgcgacgccg tctcgccggc cgtcaagccg tacgaggtct
    10441 gggtctcgct cgtcctgttc accgccatct acctggcgct gttcttcgtc tttctgtatg
    10501 tgctcgatcg caagatccgg cagggccccg aaccgctgaa gccggacgat ctggccggcg
    10561 gaccgcaccc gccggaggac ttcctcgacg ccatcagccg ccggggcaag atgcagttcc
    10621 acgcgaccga ggaagcgcag gaaggggcct gagatgatcc tcaacgaaat ctggttcctg
    10681 ctggtcgggg tgctgctgat cggctacgcg atcctcgacg gcttcgacct gggcgtcggc
    10741 gcgctccacc tgttcagccg cggcgacacc gaacggcggc tcctgatcaa cgcgatcggg
    10801 ccggtctggg acgggaacga ggtctggctg gtgacggccg gcggggcgct gttcgcggcc
    10861 tttccgcatg cctacgcgac ggcgttctcg gcgttctacc tgccgttcat cctgctgctg
    10921 ttcatgctga tcttccgcgc cctcgccatc gaattccgcg gcaaggagcc catgggctgg
    10981 tggcgccgga tgtgggacgt gtcgttctgc gtcgcgagca tcgtcgcctc ggtgctgttc
    11041 ggcgtcgcca ttggcaatat ggcgctcggt atcccggtcg gccccgacat ggaataccag
    11101 ggcaacctgc tcacgctcct cgggccgtat ccgctgctgg tcggcgtgtt caacctgagc
    11161 attttcacga tgcacggcgc gatctatctt tacatgaaga ccgagggcga cctgcagcgc
    11221 aaggtgaagg gctggatcgt ccgcgccact gcgtttttca ttatccttta cgtgctgacg
    11281 acggtttaca cgctcgcggc ggtcccgtcg atgatcgcca acttccggga ttatccgtgg
    11341 atctgggggc tcgtcgtgct taacgtgctg gcgatcgcga acatcccgcg cgcgatctat
    11401 cacgaatggc cgttccgcgc gtttctcagt tcgtgcgccg ccatcgccgc gctgatcagc
    11461 ctgttcggca tgggggtcta tcccaacctg atccgctcga cgctcggcga tgcgttcaat
    11521 ctgacgatcg tcaatgccgc ctcgtcgcag aagacgctcg gcatcatgct gctcatcgcc
    11581 gtcatcggca tcccgttcgt cctggcctac accgccgcga tctactggat tttccgcggc
    11641 aaggtgcggc tcggggagca cagttattag aacttgccgc cgtcgcggcg accgacggcg
    11701 ccggccactg ggtcgccgtc tggcagtcgg tcgacacgct ggacggaacg atcggcgcgg
    11761 attacgacat cctgttccgt cgcaatgagg ggccgtcccg ggccgggcgg ccgtaccggg
    11821 tggggcggac cgattcctcc cctgagagcc ggcgcccgcg tcgggcccac atgaggttgc
    11881 ggggaagggc cggcggcctg ccgcgaagga attctccccc ttttttcgtg cgcttggggc
    11941 cggggttccc gtaaggtatg ggcttggctt gcgccgaccg gcatgcgccg ccggcggccg
    12001 ggcccggtgc ggcggcgccg catccttgac tccaccacgc cgcgcggccc caccggtcgc
    12061 gaacgccgac catgccgctt ctgatcttca acacccagac ccgccgcaaa gagccgttcg
    12121 agccgctcga tcccccgcgc gtgacgatct acaactgcgg cccgacggtc tacgaccact
    12181 tccacatcgg caacgcccgc aacttcgtcg tcatggatct ggtccggcgc tatctccagt
    12241 atcgcggcta tcagccgttc ttcgtccaga acctgaccga catcgacgac aagatcatca
    12301 gccgcgccca gcaggaaggg gtcgcctgcg aggacatcac cggacgctat atcccgagct
    12361 atttcgccga cgcggccaag ctgcgcgtgc gcatggccga tgcccacccg cgcgcgaccg
    12421 attatgtggc cgacatcatc gggctgatcg aacggctgat cgcgcgcggc ctggcctacg
    12481 cggccaacgg atcggtctac ttccgcgtca aggcgttccg ggaatacgga aagctgtcgg
    12541 gccgccgcgt tgaggatctg ctggaagggg cgcgcgtcga ggtcagcgac gagaaggaag
    12601 atgccgcgga cttcgccctg tggaaagcgg ccaagccggg cgagccgacg tgggacagcc
    12661 catggggacc gggccggccc ggctggcaca tcgaatgctc ctgcatggcg atgaaactgc
    12721 tcggcgagac gatcgacatc catgccggcg gcaccgacct gatgtttccg caccatgaaa
    12781 acgagctggc gcagagcgag ggggcgaccg gccggccgtt cgcgcgctac tggatgcaca
    12841 atggcttcct caacatcgac agccagaaga tgagcaagtc gctgggcaat ttcctgaaga
    12901 tcgatcaggt tctgcagcgc gcgtcggcgc ccgccgtgcg ccattttctc ctttcggcgc
    12961 actatcgcaa cccgctggat ctgaccgatg aggcgctgga gcggtcggcg gcggccgtgc
    13021 ggcgaatcaa cgacgggatc gagacggcgg cgaaattgct ggcgctcgaa cgcgccgaag
    13081 cagccgcggg cgacgtcgcc gcctaccgcg ccgaattcga agcggcgatg gacgacgatt
    13141 tcaacacgcc gcgcgcgctg gcgctgctgt tcaagctggt gaccgagatt caccagttgc
    13201 gcgcggatgc ggtccacgct gccggcgaag cgaaagccga cgtgctcggt cggctggccg
    13261 cgctggtcga gttcgcccgg gagctgcgcg cgttcttcag cctcgaacca gaggaagagg
    13321 agacggacca cgcatccgcg gacagcctga ccgagtcgct gatggaactg ctgattgaaa
    13381 cgcgcgcggc ggcgcgcaag gcccgcgcct tcgcgatcgc cgaccgcatc cgcgaccggc
    13441 tgggcgaact cggcatcgcg ctggaggacc acccgcaggg gacgatctgg aagaagaagg
    13501 aaagctgacg cgacgcgtac gggaggcgcg gggggccgcg gccgccggcg cgcgcgcggc
    13561 cgccgcagtc gccggcgacg ccttccgccg cgccattcaa gccatcgaat cggggagtca
    13621 tggcgccagt tcccgacatg caatacgccg aggttcgcgc gcggtgcggg gcgtgcattc
    13681 atcccgcgcg gtcggcgggt caggccgacc tgtatgagct ggactgggag ggtcgcccgg
    13741 cgctgctgaa ggacttcagc cagcgcccgc ggctggtgcg ctggcttttc tcgcgccgga
    13801 tcgccgcccg cgaagtccgc atgctgcgcc ggctggaggg gctggaggga acgccgcgcc
    13861 tgctggcgac ggccggaccc gaagcattcg taatggagcg gctgccgggc gaacgcatcc
    13921 ccaatcgcaa acggggaggg ccgccggcta tcttctgggc caatgcgcgc gccctgctcg
    13981 accagttgca tgcgcggggg gttgcccacg gcgatctgcg ccgcaaaaac atccttgcag
    14041 gtcccaacgg cgaggcctat ctgatcgact ttgccacggc catccgtcgc gacacccccg
    14101 ggggggggcg aggttcctgg ctgggcggtt acctgttcaa acgctgccgg acgattgatc
    14161 ggaacagctt cgcccggatc aaggcgcagt acgcgcccga ggcgctcgat gcgaccgagc
    14221 ggcgctggct ggaggagatg ccgctctacc tgcgcgtggg acggatgttg aagaagcgcg
    14281 tctaccggct gcggaagcgc cgatattggc gcaagcgtgt aaaatgggtg aaatggctgc
    14341 ggcggaaact gcgggcgcgg aaaagacaag gccggcgggg gtcgcgcggt tgaggggcgt
    14401 aattcgaaga aaatccaatg aaatccgaca gattggacgg gtccggcgga tctgaccgat
    14461 attgaacggg taagggggcg gcgccgcgcc cggcaaccgg cctgattttc gctactcccg
    14521 ccggaagtga acccttccga tcgaggaggc gccccatgag gcaactgacg ccgtggctgc
    14581 tgctcctggc gatgagttcc gggttggccg gctgctactc cctgcgccat acgacggcgt
    14641 cgggcaagcc cgtgatggcc gacaccgcgc tcgccgaaaa ccaggaaatc acgcagcact
    14701 tcaaggagac cgcgcgcagc gtctatctgc tcgacggcct gatcccgctc gaccgcgacg
    14761 acgcccaggc gctgctctac gagtacgtga acgcgggcga cggcatcgtc aacctgcgca
    14821 tccgcgagca gttcacgttt attgattcgc tcatcggctg ggtgacgctg gggatcgtga
    14881 cgacgcgcac ggtgcgcgtg gaaggcgatg ttttcatcaa tcgaacggtg gcgtcggccg
    14941 cgcccgtcct gattgaagtg gacgcggagc acccg
//
LOCUS       DAOPCO010000072        14893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4067_length_14893_cov_6.176978, whole genome shotgun sequence.
ACCESSION   DAOPCO010000072 DAOPCO010000000
VERSION     DAOPCO010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14893
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4067_length_14893_cov_6.176978"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4494)
                     /locus_tag="PLS90_07070"
     CDS             complement(<1..4494)
                     /locus_tag="PLS90_07070"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPK02204.1"
                     /translation="MILQPGQSIADGLYEIESFIAHGGMAEVWRVIHKPTGQIQALKI
                     LKKKSLRFPDLLERFRLEYDMLKRLNHPHIMKAYEFGQFNYMGSQLPWFTMEYFTNNM
                     RQRLQQISIGEGLNLLLPVLEALDYAHQQNICHRDLKPMNILLNDQGRAVLTDFGIAK
                     ETDRDRGLTGRNIIGTAYYISPEQCMGLKVTPQSDIYAFGVILYQLCTGHFPFEDASE
                     VSIIRRHINETPRSVQEINPAISPTLALAIAKCLEKKPEDRWGSARELAQALRKADEI
                     AETAQHVLRRGQLFHNRRFKVIGLIDMGGFAEVYRVRNLENGRLHALKICLPALAYDS
                     EMLKRFQREITILRAFNHERIIRVVEWGEYEYENLKLPYFIMPFYPGNLGACMSQPVD
                     PEKAFQILMDVLEALAYAHSFEGTGVLHRDLKPSNILIDTEGRGYLTDFGIAKISKGV
                     STLMTRSATMTRAAVGTSYYMSPEQIRNEPVDARADIYSFGIILYEVAAGRRPYDAKS
                     SEHIIAMHLFEKPDPPRKYNLLISPRLQSVILKCLEKNPDDRFPSVPDLIEALKKTQR
                     RGKFFAYGGAADTGTISRIRKPMRRAGRSLRRAAFSPRTWLILAGVVGGVLLGWGGAV
                     AARMQVSGNPSVSQILSPRALSYDGTIRPARRMAAVFDGLGGAAAGAAAGQWEEPVEV
                     AFLDTAARQRYDELKRFREEAAAAGEITDALRAEGRMRSLAAAAAPTVAVRNPDEIIP
                     TNQGAADAVMELALSRAVDLPLQYEYRLIDQASNEVVKEGTSPTPQIQLAAVPAGSYT
                     FEARAVHQGFRTPPAQSSLEIRNVEKPIRLALQGEGDQRTVVFTNFDEGFRSYEWQVR
                     DAAGQVVPLPPDTANQPFDVALLTQDGRYQVSVLAYDQDPSIVNPYPSLPESVIVDRT
                     APQLTMTAQRGPERLGAVQPGQTFVLMPGDDPITLGFAPSDNIAAANEIRVLDAEGGQ
                     PATVLAYNPDRSTYAFVVEDPYGNRSQPFEIRVQSVGQEMAALAGQWSEAQTRLNADA
                     TRYARTRALFDPGPLAQAVSGGSISREWLAAQDESAMEQLQQKLDELEDLMERDDAFA
                     EQADLLAAVAAQLPAAQQGPLNAALRQARQSWATDVAAADLEPTQLLREAARAGLPAV
                     TREMLAYAPAGDTGAWRLELSGAAPRLPNGRYRYSLAQEDPGSADAAQLLSGNTTSEA
                     DISVEVARPDQPVNLAAQFVIDAGEPLGNLAGPPSLIHFDPAVQIDDQLRADMDNYEA
                     LFQRLARDEARYARTLAWLQQPGRLSLVDRSGNFDFAWTLRNANDPAQIQQMREQLQT
                     LQQMAAQDDAFAEALENVDLAGWSARHRNALADTLRDAWMKGGAQDVAAWIGAQRNEY
                     AARNADTLLAPPEVDWDAISVELAEKIVRIERRAFGKPPTDSASLKLGWTTGDSAPAS
                     TFDLTGDSTTAEPPQLGREYRLAARWVSGDYASDWAIGGRYEIPDATPTPTPTPSPTA
                     TPTP"
     gene            complement(4491..6032)
                     /locus_tag="PLS90_07075"
     CDS             complement(4491..6032)
                     /locus_tag="PLS90_07075"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HPK02205.1"
                     /translation="MQFEHFALTHTGLVREMNQDSILALPRCGVFMVADGMGGEKAGD
                     EASAQVVKTTQAAVEAFFKSKPTGPSQIENMLRDTLLEANHEVFQISVREPAKRGLGS
                     TASLLCLHRGVYFVAQVGDSRVYRLRDGAAVQLTHDHTLVWLLYEQGNITRDQLETHP
                     ERHLLTQCIGSQKPVKVDVFEGDLQVGDTFLICSDGLTGYAREEKVLEILGETEISLE
                     QRAGMMVDAALEAGGGDNVSVILVKITQLDAEDNWEPEATAPPTEFTEDTVDMLIDDE
                     MRPQARPRRRRAGLLWGALVAVLAAGALFFLLPREAVGPNAIAVYLEPAEGVALPAGA
                     VTATIVSTAADTTASQTLQTEDDAAPSFTVPGPGEYQVTLQSAGYAPLTLPLTVPAAE
                     PAVERVSLPIAEWTRYGTYTLDYGDPSQLSRVRLFRADANGRVPFDRAAELTVTPDQL
                     AGQSRLSFDLLPGEIYLVQVDVAGRRPITHEKISVDSGETVVYELNLPPPLRADNPTT
                     GGSQP"
     gene            complement(6071..8467)
                     /locus_tag="PLS90_07080"
     CDS             complement(6071..8467)
                     /locus_tag="PLS90_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02206.1"
                     /translation="MKKPAAPGPGSAPRRDEDDAPAPESTRRLDADDLEHADEPSLED
                     ADEAGDERQATMRLDDDDLAAAEADLTEPEADSAAPQATARFEPDQIAEPLDELTGQA
                     TRRLEEPPESFDAQATRRLDLDDSALPPAGVQAEPELEPPASPAEERTPPGGFEGIEL
                     DLEPAPRPGAEPTVRLHQEGEGLTDPGMQRTQRIDGVEEFQFDELPADDPTAARERTV
                     VEPLAHAEDDDRHVTLEDTSPEALARTRADVQRVGQLREELPRPAPPAGQPRPAPPAD
                     MEPIRPEEEEVPKPFNLQPEDPELIEMATHATQDSRGAAPVRHKWLWPLIGIVIGLAL
                     VGWGYMISWSIPFEPPAPQHEMLEVERFGQFVPPEKWVLEKDLFIGRDATLFQQPWTY
                     DPTAASPMKRGTQRAPFDPAAGLFTLAGAPPLETLTQLAWIRDSRGEEAAADAATTAA
                     AQMPTGYLLEWREYDFLTAEQTRNGIQGTIVAAPPPILLPDTGYAFPPVAVDGAVAEG
                     TLLVVVDQQGALTAWPMARGETSEPRWRAQLEGEPLAEPWLMLQGDGDEVNYWLVAAT
                     TAGLQSIAAASGEPAGRLAWSAPLAADRRVAMQPARAGADRWGGFVSDGRQLLLFALN
                     DAGAPQAVWQATHDDDLGQPGAPPHLLSFVDGSRPDHDVLGVVAADGRIALYPAASRE
                     GQTPPRAIAGPGPAVTAMDVNNDAHWDLIGLTREAGQFWVAPGPEFEPLAADETPPID
                     EATAPGSDVLWRLHETQLIAEYFDGAGGPHRIALPLPAMKKDVLELFQRDWKRRHERF
                     "
     gene            complement(8633..10516)
                     /locus_tag="PLS90_07085"
     CDS             complement(8633..10516)
                     /locus_tag="PLS90_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HPK02207.1"
                     /translation="MSIQSTKIGFGAGSGGLTPQSVLPYRLLVVADFLSKRRALDGSD
                     PLPNPRRVDKDNLNAVFAELAPTVALEVPNHLGGPAPTIEHEFTFRSFDDLRPEALAA
                     AHPGCAALLALRRQIDELARQGQSAGQIAARLEGELRGRPYGPEVLAALEPGAQGASA
                     GAAGGDGSQPDPSAGNGGASLLDDLLEETEKNQSGKSATSGASMGSVLDSLVGGAGKK
                     KPGSGVAAALDALDQRLGRQLDAILHDRRIAELEEAWRGLRFLVSRTDFRRDIQLEIL
                     PCAKDDLRAVLVQHVMEDEYNMPGDPPLACIIVNHAFDRTARDTNALQELTQAAERLQ
                     APLLAEAAPQFIGVDSLNDLGAGGSLHDLLSHPEFAKWLGLRNTDPSRWLAICMNRFL
                     LRRPWGEEETRVKGFNYREQTNEAKGENLCWGNAVWAVASRITDSFAREGWPQFISGA
                     RAEGLLEDLPLRKWEPKPGRGVQITLETGLSEMHAQDLATAGILSLQCAANKDAAYIT
                     YVPTAHLAKRYPDLGQTQDAALKAILPYQLYAGRVALALMRFLGEVGGGDPDSAQQAL
                     KQRLLDLLSTSKGAAPDHCVNVAVADNPDDPMRYLATIEIRPPFKLLNMTPRLELTVP
                     LAK"
     gene            complement(10668..12503)
                     /locus_tag="PLS90_07090"
     CDS             complement(10668..12503)
                     /locus_tag="PLS90_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPK02208.1"
                     /translation="MTDEILLLPMPQRLQVLPGRFRLRDDALLLLKGEPRAELLRLGH
                     TLRGTLRTLGKSPALTTFGVSTDPRVQVLLAIDPAQVRQPEGYRLEITPAQLSIIAHD
                     GAGAFYAAMTLRQLARWEADSLPALRIEDWPDLPNRGVMLDVSRDMVPRMGTLMNLID
                     SLAEWKINQFQLYTEHTFAYRDDGAVWADASPFTPQQILDLDQYCRERFIQLVPNQNS
                     FGHMERWLRHAPYRALAELPEGESATLNPLDPGSIQLVARLYQELLPHFTSRLFNVGC
                     DETFELGKGRSRAECERRGPGRVYLDYLLKIHRLVQHHGRTMMFWGDIIQQHPDLIAE
                     LPRDVIALEWGYEHDHPFPERCEKYVRAGVPFYICPGTSSWCSFAGRTDNAVANLRAA
                     ADNARQFGASGYLITDWGDYGHLQYLPTAWLGFACGAALAWSLEANDDLNLPRALDRH
                     VFLDRARRMGRIVHQMGNVYTLAGERLRNRSMIFQQYFRPGDPVQDEIRYRMRPHHGE
                     QAIEALDELMNGLHQTAMQRPDAQLLIDEFTQAGAFLKHGCHYAIARGGGTAADLDAA
                     ARRELTAELTRLIAEHRRLWTFRARSGGLEASVARLEAQLQVYRQ"
     gene            13093..14049
                     /locus_tag="PLS90_07095"
     CDS             13093..14049
                     /locus_tag="PLS90_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK02209.1"
                     /translation="MVEDSRISDETRQRILQQARLLNYIPNSAARKLRHGRSKISVIG
                     FLINDITHPFYSLMVQAAEKTAARHGYEILVGDTQWNPAKEVAEVKHMIESRVDGVLA
                     CLSERTRESTGLLDGFAVPYLMLDTTPREYRGACVVNDLAEASRIAVSHLIQVGCRRL
                     AFLAADESMAHFSAFTKLREEFARAVRARGLPFEPSQIIRAGLSIEAGQSAFEEALRV
                     CPGVEGIFCVNTLCALGVIEAADRRGVRVGREVAVMGVDDLDICSLSRISLTTIRQPY
                     DQLAELATEYLVGAIENGAAPTMRQSLKPELVARQSTRFGNR"
     gene            14377..>14893
                     /locus_tag="PLS90_07100"
     CDS             14377..>14893
                     /locus_tag="PLS90_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02210.1"
                     /translation="MNILLKTTRRNQFMGHHLFTHFQKAGLGCFWRLTVFLGMLAFAA
                     TAWCEAPAAGNILIGPGGRQARILVSPPAPLARLMAGRLADYLEQRTGVRPQLISALP
                     KEGTEGVVFLLAQADNRAVSKLAADSALALPDMKAWREDGYALRTLAQGKTQYVLCAG
                     KGDSGVKYAVYR"
BASE COUNT         2260 a         4737 c         5365 g         2531 t
ORIGIN      
        1 cggcgtgggg gttgcggtcg gcgatggcgt cggcgtgggc gtgggggtgg cgtcggggat
       61 ttcgtagcgg ccgccgatgg cccagtccga ggcatagtcg ccgctgaccc agcgggcggc
      121 gagccggtat tcgcggccga gctggggggg ctcggcggtg gtggaatcgc cggtcaggtc
      181 gaacgtgctg gcgggggcgc tgtcgccggt ggtccagccc agcttgaggc tggcggagtc
      241 ggtgggcggc ttgccgaacg cgcgccgttc gatgcgcacg atcttctcgg ccagttcgac
      301 cgagatcgca tcccagtcga cctccggcgg cgccaggagg gtgtcggcgt tccgcgcggc
      361 atactcattg cgctgcgcgc cgatccacgc ggcgacgtcc tgggcgccgc ccttcatcca
      421 ggcatcgcgc agcgtatcgg ccagggcatt gcggtgccgg gcgctccagc cggccaggtc
      481 gacgttctcg agcgcctcgg cgaaggcgtc gtcctgcgcg gccatctgct gcagcgtctg
      541 aagctgttcg cgcatctgct gaatctgggc cggatcattg gcgttgcgca gcgtccaggc
      601 gaagtcgaaa ttgccggagc ggtcgacgag tgagaggcgg ccgggctgct gcagccaggc
      661 cagggtgcgc gcgtagcggg cttcgtcgcg cgccaggcgc tggaacagcg cctcatagtt
      721 gtccatgtcg gcgcgcaact ggtcgtcgat ctgaacggcc gggtcgaagt gaatcaggct
      781 gggcggcccg gccaggttgc ccagcggttc gccggcatcg atgacgaact gcgcggcgag
      841 attgaccggc tgatcgggcc gagcgacctc caccgagata tcggcctcgg acgtggtgtt
      901 gcccgagagg agctgcgcgg catcggccga gccgggatct tcctgagcca ggctgtaacg
      961 gtagcggcca ttgggcaggc gcggggcggc gccggaaagt tccaggcgcc acgcgccggt
     1021 gtcgccggcg ggcgcatagg ccagcatctc gcgcgtgacg gcgggcaggc cggcgcgggc
     1081 ggcttccctg agcagttgcg tcggctccag atcggcggcg gcgacgtcgg tcgcccagct
     1141 ctggcgggcc tgccgcaggg cggcgttgag cggcccctgc tgcgcggcgg gaagctgggc
     1201 cgccacggcg gcgagcaggt cggcctgctc ggcgaacgcg tcgtcgcgct ccatcaggtc
     1261 ttccagttca tccagcttct gttggagctg ttccatcgcg ctctcatcct gggccgccag
     1321 ccactcgcgg ctgatcgagc cgccggagac cgcctgggcg agcgggccgg gatcaaacag
     1381 cgcgcgggtc cgggcgtagc gcgtcgcatc ggcgttcagg cgcgtctgtg cctcggacca
     1441 ttggcccgcc agcgccgcca tttcctggcc gacgctctgg acgcggattt caaagggctg
     1501 gctgcggttg ccgtacggat cctcgaccac gaaggcatag gtggagcgat ccgggttata
     1561 ggcgaggacg gtggccggct ggccgccctc ggcgtcgagg acgcggattt cattggcggc
     1621 ggcgatattg tcgctggggg cgaagccaag cgtgatcggg tcgtcgccgg gcatcaggac
     1681 gaaggtctga cccggctgga cggcgcccag ccgctcgggc ccgcgctgcg ccgtcatcgt
     1741 cagttgcggc gcggtccggt cgacgatcac cgactcggga aggctggggt acgggttgac
     1801 gatcgagggg tcctgatcat aggccaggac gctgacctga tagcgcccgt cctgagtcag
     1861 caacgccacg tcgaacggct ggttggcggt gtcggggggg agcggaacga cctggccggc
     1921 ggcatcgcgg acctgccact cgtaggaacg gaagccctcg tcgaaattgg tgaagacgac
     1981 ggtgcgctgg tcgccctcgc cctgcagcgc caggcgaatc ggcttttcga cgttgcggat
     2041 ttcgagcgag gactgggccg gcggggtgcg gaaaccctga tggacggcac gggcctcgaa
     2101 cgtgtagctg ccggccggca ccgcggccaa ctggatctgc ggcgtgggcg acgtgccttc
     2161 cttgaccacc tcgttgctgg cctgatcgat caggcggtat tcgtactgca ggggcaggtc
     2221 gacggcgcgc gagagggcca gttccatgac ggcatcggcg gcgccctggt tggtggggat
     2281 gatctcgtcc ggattgcgga cggcgacggt cggcgcggcg gcggcggcca gcgagcgcat
     2341 gcgtccctcg gcgcgcaggg catcggtgat ttcgccggcg gcggcggctt cctcgcggaa
     2401 acgcttcagt tcgtcgtagc gctggcgggc ggcggtatcg aggaacgcaa cttcgaccgg
     2461 ttcctcccac tggccggccg cggcgccggc cgctgcgccg ccgagcccgt cgaagacggc
     2521 cgccatgcgg cgcgccgggc ggatcgtgcc gtcgtagctt agcgcgcgcg gcgagaggat
     2581 ctgactgacc gacggattgc cgctgacctg catgcgggcg gccacggcgc cgccccagcc
     2641 cagcagcacg ccgccgacga cgccggcgag gatcaaccag gtgcggggcg aaaacgcggc
     2701 gcggcggagc gagcggccgg cgcgccgcat cggtttgcgg atgcgggaga tggtgccggt
     2761 gtcggccgcg ccgccatagg cgaagaactt gccgcgccgc tgcgttttct tgagcgcttc
     2821 gatcagatcc ggaacgctgg ggaaccggtc atcgggattc ttctccaggc atttgagaat
     2881 caccgactgg agtcgcggcg agatcagcag gttgtatttg cgcggcggat cgggcttctc
     2941 gaacaggtgc atcgcgatga tgtgttcgct gctcttggcg tcgtagggac gccggcccgc
     3001 cgccacctca tagaggatga tgccgaagga gtagatgtcg gcgcgggcgt cgaccggttc
     3061 gttgcgtatc tgctcgggcg acatgtagta gctggtgccg acggcggcgc gcgtcatcgt
     3121 ggcgctgcgc gtcatcagcg tggagacgcc cttggagatc ttggcgatgc cgaagtcggt
     3181 caggtagccg cggccctcgg tgtcgatcag gatgttggag ggtttcaagt cgcgatggag
     3241 gacgccggtt ccctcgaacg aatgggcata ggccagcgcc tcgaggacat ccatcagaat
     3301 ctggaaggcc ttctccggat cgaccggctg cgacatgcag gcgccgagat tgcccggata
     3361 gaacggcatg atgaagtacg gcagcttgag gttttcgtat tcgtattcgc cccactcgac
     3421 gacccggatg atccgctcgt ggttgaaggc gcgcaggatc gtgatctcgc gctggaagcg
     3481 cttgagcatc tcgctgtcat aggcgagcgc gggcaggcag atcttcaggg catggagccg
     3541 gccgttctct agattgcgga cgcgatagac ctcggcgaag ccgcccatgt cgatcaggcc
     3601 gatgaccttg aagcggcggt tgtggaacag ctggccgcgg cgcaggacgt gctgggcggt
     3661 ctcggcgatt tcgtcggcct tgcgcagggc ctgggccagt tcgcgggcgg agccccagcg
     3721 atcctcgggc ttcttttcca ggcacttggc gatggccagg gcgagcgtgg gcgagatcgc
     3781 cgggttgatc tcctgcaccg aacgcggcgt ctcgttgatg tggcggcgga tgatcgagac
     3841 ttccgacgcg tcctcgaacg gaaagtggcc ggtgcagagc tgatacagga tgacgccgaa
     3901 ggcgtagatg tcgctctgcg gcgtcacctt caggcccatg cactgctcgg gcgagatgta
     3961 gtaggcggtg ccgatgatgt tgcgcccggt gaggccgcga tcacgatcgg tttccttggc
     4021 gatgccgaag tcggtcagca ccgcgcgccc ctgatcgttg agcagaatgt tcatcggctt
     4081 caggtcgcga tgacagatgt tctgctggtg ggcgtagtcg agcgcctcga ggacgggcag
     4141 cagcagattg agcccctcgc cgatcgagat ctgctgcagg cgctggcgca tgttgttggt
     4201 gaagtactcc atggtgaacc atggaagctg cgagcccatg tagttgaatt gcccgaactc
     4261 gtaggccttc atgatgtgcg gatgattgag ccgcttcagc atgtcgtatt cgaggcggaa
     4321 gcgttcgagc agatcgggga agcgcagcga ttttttcttc aggatcttga gcgcctgaat
     4381 ctggccggtc ggcttgtgga tgacgcgcca gacctcggcc atgccgccgt gggcgatgaa
     4441 cgactcgatc tcgtagagac cgtcggcgat ggattgaccg ggttgcagaa tcatggctgg
     4501 ctgccgcctg tggtcgggtt atccgcgcgc agcggcggtg gcaggttgag ttcgtagacg
     4561 accgtctcgc ctgaatcgac cgaaatcttc tcgtgcgtga tcggccggcg gcccgcgaca
     4621 tcgacctgaa ccagatagat ttccccgggc aggagatcaa acgacagccg cgactggccg
     4681 gcgagctgat cgggcgtgac ggtcagttcg gccgcgcggt cgaaggggac ccggccgttg
     4741 gcatcggcgc ggaacagccg cacgcgcgac agttgcgacg ggtcgccgta atcgagcgta
     4801 taggtgccgt agcgcgtcca ctcggcgatc ggcaggctaa cccgctcaac cgccggctcg
     4861 gcggcgggga ccgtcagcgg cagcgtcagg ggcgcatagc cggccgactg caacgtcacc
     4921 tgatactcgc ccggtcccgg gacggtgaac gacggcgcgg cgtcatcctc ggtctgcaac
     4981 gtctgactcg ccgttgtatc ggcggcggtg gaaacaatcg tggccgtgac ggcgccggcg
     5041 ggcaaggcga cgccctcggc cggctcgagg tagacggcga tcgcattggg gccgacggcc
     5101 tcgcgcggca gcaggaagaa cagggcgccg gcggcgagca cggccaccag cgcgccccac
     5161 agcaggccgg cgcggcggcg gcgcggccgc gcctgggggc gcatctcgtc gtcgatcagc
     5221 atgtcgacgg tatcttcggt gaattcggtg gggggcgcgg tcgcctcggg ctcccagtta
     5281 tcctcggcgt cgagctgcgt gatcttgacg aggatgacgc tgacattgtc gccgccgccc
     5341 gcttcgagcg cggcgtcgac catcatcccg gcgcgttgtt caaggctgat ctcggtctcg
     5401 cccaggatct ccagcacctt ttcctcgcgg gcatagcccg tcaggccgtc cgagcagatg
     5461 aggaacgtgt cgcccacctg caggtcgccc tcgaagacgt cgaccttgac cggtttctgg
     5521 ctgccgatgc actgagtcag caggtgccgc tcgggatgcg tctcgagttg gtcgcgggtg
     5581 atgttgccct gttcgtagag cagccagacg agcgtgtgat cgtgggtcag ttgaacggcg
     5641 gcgccgtcgc gcaggcggta gacgcggctg tcgccgacct gggcgacgaa atagacgccg
     5701 cgatggaggc agagcagcga ggcggtggag cccagcccgc gcttggccgg ctcgcggacg
     5761 gagatctgga agacctcgtg gttggcctcc agcagcgtat cccgcagcat gttttcgatc
     5821 tgactggggc cggtcggctt gctcttgaaa aaggcttcga cggccgcctg cgtcgtcttg
     5881 accacctggg ccgaagcttc gtcgccggcc ttttcgcccc ccatgccgtc ggcgaccatg
     5941 aagacgccgc acctgggcag cgccagaatc gagtcctggt tcatttcgcg cacgaggccg
     6001 gtgtgcgtca gagcgaaatg ttcgaattgc atgcgctgtt ccccagtggc gatacccggc
     6061 ggtgtggccg tcagaaccgt tcgtggcgcc gcttccagtc gcgctggaac agctccagca
     6121 cgtccttttt catcgcgggc aacggcagcg cgatgcgatg cggaccgccc gcgccatcga
     6181 agtattcggc gatcagttga gtctcatgca ggcgccagag cacgtcggag ccgggcgccg
     6241 tggcctcgtc gatcggcggc gtctcatcgg ccgccagcgg ttcaaactca ggtcccggcg
     6301 ccacccagaa ctggcccgcc tcgcgcgtca gtccgatcag atcccaatgg gcatcgttgt
     6361 tgacgtccat cgccgtgacg gccggccccg ggccggcgat ggcgcgcggg ggcgtctggc
     6421 cctcgcgact ggccgccgga tagagcgcaa tgcgtccgtc ggccgcgacg acgcccagca
     6481 cgtcgtgatc gggccggctg ccgtcgacga acgagagcag atgaggcggc gcgcccggtt
     6541 gccccaggtc gtcgtcgtgc gtcgcctgcc agaccgcctg cggcgcgccg gcgtcattca
     6601 gcgcgaacag gagcagctga cggccgtcgc tgacgaaacc gccccagcga tcggcgccgg
     6661 cccgcgccgg ctgcatcgcg acgcggcggt cggccgcgag gggggcggac cacgcgagac
     6721 gcccggccgg ttcgcccgac gcggccgcga tggactgcaa tccggcggtc gtcgccgcga
     6781 ccagccagta gttcacttca tccccgtcgc cctggagcat cagccagggt tcggcgagcg
     6841 gctcgccctc gagctgggcg cgccagcgcg gttcgctcgt ttcgccgcgc gccatcggcc
     6901 aggccgtcag cgcgccttgc tgatcgacaa cgaccagcag cgtcccctcg gcaaccgcgc
     6961 catcaacggc gactggcggg aaggcgtagc cggtgtcggg cagcaggatc gggggcggcg
     7021 cggcgacgat cgtcccctgg atcccgttgc gcgtctgctc ggccgtcagg aagtcgtatt
     7081 cgcgccactc cagcagatag ccggtcggca tctgggccgc cgcggtggtg gcggcatcgg
     7141 cggcggcctc ctcgccgcgg ctgtcgcgaa tccaggccag ttgcgtcagg gtttccagcg
     7201 ggggcgcccc cgccagcgtg aacaggccgg cggcggggtc gaacggcgcg cgctgcgttc
     7261 cgcgtttcat cgggctcgcg gccgtcgggt cataggtcca cggttgttgg aacagcgtgg
     7321 cgtcccggcc gatgaacaga tccttctcca gaacccattt ctccggtggc acgaactggc
     7381 cgaagcgctc gacttcgagc atctcgtgtt gcggcgccgg cggttcgaac gggatcgacc
     7441 aggagatcat gtagccccag ccgacgagcg ccagcccgat cacgatgcca atcaggggcc
     7501 agagccattt atggcgcacg ggcgccgcgc cgcgcgagtc ctgcgtcgca tgcgtcgcca
     7561 tctcgatcag ttcggggtcc tcgggctgga ggttgaacgg cttggggact tcctcctcct
     7621 cggggcggat cggttccatg tcagcgggcg gcgcgggccg cggctggccg gcggggggcg
     7681 ccgggcgcgg caattcctcg cgcaattgcc cgacgcgctg cacgtcggcg cgcgtccggg
     7741 ccagcgcctc gggggaggtg tcctccagcg tcacgtggcg atcgtcgtcc tcggcatgcg
     7801 ccaggggctc cacgacggtg cgctcgcgcg cggcggtggg gtcgtcggcc ggcagctcgt
     7861 cgaactgaaa ttcctcaacc ccatcgatcc gctgcgtgcg ctgcatgccg ggatcggtta
     7921 gaccttcccc ttcctggtgc aggcggacgg tcggctcggc gcccggacgg ggtgcgggct
     7981 ccaggtccag ttcaatcccc tcgaacccgc cggggggggt gcgttcctcg gccggcgagg
     8041 cgggcggttc cagttcaggc tccgcctgga cgccggccgg cggcagggcg gagtcgtcca
     8101 gatcaagccg gcgagtcgcc tgcgcgtcga agctctcggg cggttcctcc agccggcggg
     8161 tggcctggcc ggtcagttcg tccagcggct cggcaatctg atcgggttcg aaacgggcgg
     8221 tcgcctgcgg agcggcgctg tcggcttccg gttccgtcaa atcggcctcg gccgcggcca
     8281 ggtcgtcgtc atcgagccgc atggtggcct ggcgttcgtc gcccgcctca tcggcgtcct
     8341 cgaggctcgg ctcgtcggcg tgctccagat cgtcggcgtc gagccgccgc gtgctttcgg
     8401 gggcgggcgc gtcgtcctca tcgcggcggg gggcggaacc cggcccgggc gccgcgggct
     8461 tcttcatgaa cggcagcggc gcaaactggg gtttccgctc gtcgccttgg ggagggccct
     8521 gatcatcgtg gtcgtgagga cttaccatat tccaactcgt ggccgggagc gggcaagcgc
     8581 gacgccttgg agccaggttc gggattgcgc cgcggcgcag gccgcggccg ggctacttcg
     8641 ccaacgggac ggtcaactcg agccgcggcg tcatgttgag cagcttgaag ggaggacgga
     8701 tttcgatcgt cgccaggtag cgcatcgggt cgtcgggatt gtccgcgacg gcgacgttga
     8761 cgcaatggtc gggcgccgcc cccttgctcg tgctcaacag gtcgaggagc cgctgcttga
     8821 gcgcctgctg ggccgagtcc ggatcgcccc cgccgacctc gccgaggaag cgcatcagcg
     8881 ccagcgccac gcgccccgcg tagagctggt agggaaggat cgccttgagc gcggcatcct
     8941 gcgtctgccc cagatcgggg tagcgcttgg ccagatgcgc cgtcggcaca taggtgatgt
     9001 acgcggcgtc cttattggcg gcgcactgga gcgacaggat gcccgcggtg gccaggtcct
     9061 gcgcatgcat ctcggaaaga ccggtctcca gcgtgatctg cacgccgcgg cccggcttcg
     9121 gttcccattt gcgcaggggg aggtcctcga ggaggccttc ggcgcgcgcg ccggagatga
     9181 actgcggcca gccctcgcga gcgaaactgt cggtgatgcg gctggcgact gcccagacgg
     9241 cgttgcccca gcacaggttc tcgcccttgg cttcattcgt ctgttcgcgg tagttgaagc
     9301 ccttgaccct ggtctcctcc tcgccccagg ggcggcgcag aaggaaccgg ttcatgcaga
     9361 tcgccagcca gcgcgagggg tcggtgttgc gcaggcccag ccacttggcg aactcgggat
     9421 gcgacagcag gtcgtgcagc gagccgccgg cgcccagatc gttgagcgaa tcgacgccaa
     9481 tgaactgggg ggccgcctcc gccagcaacg gcgcctggag gcgctcggcg gcctgcgtca
     9541 attcctgcag cgcgttggtg tcgcgcgcgg tccggtcgaa cgcgtgattg acgatgatgc
     9601 acgccagcgg cggatcgccc ggcatgttgt actcgtcctc catgacgtgc tggacgagga
     9661 cggcgcgcag gtcgtccttg gcgcagggga ggatctcaag ctgaatgtcg cggcggaagt
     9721 cggtgcgcga gacgaggaag cgcaggccgc gccaggcttc ctccagctcg gcgatgcggc
     9781 ggtcgtggag gatcgcgtcg agctggcggc ccagccgctg gtccagtgca tcgagcgcgg
     9841 cggcgacgcc gctgcccggt ttcttcttgc ccgcgccgcc gacgagcgaa tccaggaccg
     9901 agcccatcga ggcgcccgag gtcgcgctct tgcccgactg attcttctcg gtctcctcga
     9961 gcaggtcgtc cagcagcgaa gcgccgccat tgcccgccga cggatcgggc tgggaaccgt
    10021 cgccgccggc cgcgccggcc gaggcgcctt gagcgccggg ctcgagcgcg gcgaggactt
    10081 cgggcccata ggggcggccg cgcagctcgc cttccagccg ggccgcgatc tggccggccg
    10141 actggccctg gcgcgccagt tcgtcgatct ggcggcggag cgccagcagt gcggcgcagc
    10201 ccggatgcgc ggcggcgagc gcctcgggac gcagatcgtc gaacgagcgg aacgtgaatt
    10261 catgctcgat cgtcggcgcc ggtcccccca gatgattggg aacctcgagg gcgacggtcg
    10321 gggcgagctc ggcaaagacc gcgttgaggt tgtccttgtc gacgcgccgc ggattgggca
    10381 acgggtccga cccatccagc gcgcggcgtt tgctgaggaa atcggccacg accagcagcc
    10441 gataggggag cacggactgc ggtgtgagac cgccgctgcc ggcgccgaag ccgatttttg
    10501 tcgactggat gctcatgata tcctccgaca ttggcgaaag tgaccgacct ggcaatgtcg
    10561 ccagccgcga ccccgtacta cctcgtcgcg ccgtcgcgca gggctgagcg gtgcaatggc
    10621 acgaatctta acgcagccgc ccgctccacg caagcgaacg gttgtcttta ctggcgatag
    10681 acttgcagtt gggcttcgag gcgggcgacg ctggcctcca gcccccccga acgggcgcgg
    10741 aacgtccaca gccggcggtg ctcggcaatc agccgggtca attccgcggt cagctcgcgg
    10801 cgggccgccg cgtccaggtc ggcggcggtc cccccgccgc gcgcgatcgc ataatggcaa
    10861 ccgtgcttga ggaacgcgcc ggcctgcgtg aattcgtcga tcaggagctg cgcgtcgggc
    10921 cgctgcatgg cggtctggtg caggccgttc atcaactcgt cgagcgcctc gatcgcctgc
    10981 tcgccgtggt gggggcgcat gcgatagcgg atttcgtcct gcaccggatc gcccggccgg
    11041 aaatactgct gaaagatcat cgagcggttg cgcagccgct cgccggccag ggtgtagacg
    11101 ttgcccatct ggtggacgat gcgccccatg cgccgcgcgc ggtcgaggaa gacgtggcgg
    11161 tcgagcgcgc gcgggaggtt cagatcgtca ttggcctcga gcgaccaggc cagcgcggcg
    11221 ccgcaggcga aacccagcca ggcggtcggc agatactgga gatggccgta atcgccccag
    11281 tcggtaataa ggtagccgga ggcgccgaac tggcgcgcgt tgtcggccgc ggcgcgcagg
    11341 ttggcgacgg cgttgtcggt gcgcccggcg aagctgcacc agcttgaggt gccggggcag
    11401 atatagaacg ggacgccggc gcgaacgtat ttctcgcagc gttccgggaa cgggtggtcg
    11461 tgctcgtagc cccattccag cgcgatgacg tcgcgcggca gctcggcgat caggtcgggg
    11521 tgctgctgga tgatgtcgcc ccagaacatc atcgtgcggc cgtggtgctg gacgagccgg
    11581 tggatcttga gcaggtaatc caggtagacc cggcccgggc cccggcgctc gcattcggcg
    11641 cggctgcgtc ccttgccgag ctcgaaggtt tcgtcgcagc cgacgttgaa caaccggctg
    11701 gtgaaatgcg gcagcagctc ctgatacagc cgcgcgacga gctggatcga gccgggatcg
    11761 agcgggttga gcgtggcgga ctccccctcg ggcagctcgg cgagcgcacg gtagggggcg
    11821 tggcgcagcc agcgttccat gtgcccgaac gagttctggt tggggacgag ctggatgaac
    11881 cgctcgcggc aatactggtc caggtcgagg atctgctgcg gggtgaaggg cgaggcgtcg
    11941 gcccagaccg cgccgtcgtc gcgataggcg aacgtgtgtt cggtgtacag ctggaactga
    12001 ttgatcttcc actcggcgag cgagtcgatc aggttcatca gcgtccccat gcgcgggacc
    12061 atgtcgcggc tgacgtcgag catgacgccg cggttgggca ggtcgggcca gtcctcgatg
    12121 cgcagcgcgg ggagcgagtc ggcctcccag cgggcgagct ggcgcagcgt catcgcggcg
    12181 tagaacgcgc cggcgccgtc gtgcgcgatg atcgaaagct gggcgggcgt gatctccagg
    12241 cggtaaccct cgggctggcg cacctgggcc gggtcgatcg ccagcagcac ctggacgcgc
    12301 gggtcggtcg agacgccgaa cgtcgtcagc gcgggggact tgccgagcgt gcgcagcgtc
    12361 ccgcgcagcg tgtggcccag gcgcagcagc tcggcgcgcg gctcgccctt gagcaacagc
    12421 agggcgtcgt cgcgcagccg aaaccgcccc ggcaggacct gcagccgctg cggcatcggc
    12481 agcagcagga tctcatcggt catcggttcg gatccgggcg agggggccat cgggccggtg
    12541 tcgggcgaga gggaatgcat gcggcgcctt tgcggggagt ggattgggag ggagtgaaga
    12601 gagaaattcg gatccggttc ggccgctcag agaccgcgag tcgaattcgc catcccatga
    12661 tgccgcagcg gccagtgact ggcaagaagc cgacgcggca gcgggcgaac ggaacggctg
    12721 cgcggagggg aggccctgac gcccgaaaac cccggcaatg ttgatcagtt ggggatcggt
    12781 catcatgcgt ggatgcccgc aacccgaaat ttgtcaagtc tttcatggac gtgatatcga
    12841 agggtgatat aaacaggtcc agtaagcccg ccggacctct gccgcaaggc atgctcactt
    12901 cggtgggtct ttctttttgg agtgcggcgc ggtgatgaaa tacaccaagc tcacccttcc
    12961 ttcgagcaac aggcggatcg gctgatcagt cggggttggt cagggccagt cttccgatac
    13021 tcgaaccgct cattaataaa aaacccgtcg ccccggagaa tgactccgga gagacgggtt
    13081 ggtcttttct aaatggtgga ggactcgcgc atcagcgacg agacgcgcca gcgcatcctc
    13141 caacaagccc gcctgctcaa ttacatcccc aactcggccg cgcgcaaact gcgccacggc
    13201 cgctccaaga tcagcgtcat cggcttcctg atcaacgaca tcacccatcc cttttattcg
    13261 ctgatggtcc aggccgccga gaagacggcg gctcgccacg gctatgagat cctggtcggc
    13321 gacacccagt ggaatccggc caaggaggtg gccgaggtca agcacatgat cgagtcgcgc
    13381 gtcgatgggg tactggcctg cctctcggag cggacgcgcg agagcaccgg cctgctcgac
    13441 ggcttcgccg tcccctacct gatgctcgac accacccccc gcgagtaccg gggcgcctgc
    13501 gtggtcaatg acttggccga ggccagtcgc attgccgtca gccatctgat ccaggttggc
    13561 tgtcgccgcc tggcctttct ggcggccgat gaatcgatgg cgcacttcag cgcctttacc
    13621 aagctgcgtg aggaatttgc gcgcgccgtg cgcgcgcgcg gcctgccgtt tgagccgagc
    13681 cagatcattc gcgccggcct gagcatcgag gccggccaga gcgcctttga agaggcgctg
    13741 cgggtgtgtc ccggggtcga ggggattttc tgcgtcaaca cgctgtgcgc gctgggggtg
    13801 atcgaggccg ccgaccgccg gggcgtgcgc gtgggccgcg aggtggccgt catgggagtc
    13861 gatgacctgg acatctgcag cctttcgcgc atctcgctga cgactatccg ccagccctat
    13921 gaccagctcg ccgaactcgc cacggaatac ctggtcggcg ccatcgaaaa cggcgccgcg
    13981 ccgaccatgc gccagtcgct caagcccgaa ctggtcgcgc gccagagcac gcgcttcggg
    14041 aaccgttaag ttgctgccgg gctgggcggc gtcaagcgcg ggagaggtat ctgtcgccgt
    14101 ttggtgaaac atggttcttg actatgttaa atgcatttaa ttatcatcgg cgcgcccttg
    14161 acaaggacaa accattctag tcgggatata ggtaaaagga ccgccttcgc accgaaacca
    14221 tggctttccc ggagctggtc ggggccttcg gtgttgtttt acaatttttt gctcatcgcg
    14281 gccatgggac cttaagctcg tttggaaaaa gtgggctggg ccaaaccttt tgatattcag
    14341 gttagtctcc ttggtaaatg cttttaacag gcgttcatga acatcctatt aaaaaccacg
    14401 cgcaggaacc aattcatggg acatcacctt ttcacgcact ttcagaaggc gggcttgggc
    14461 tgtttctggc ggttgaccgt tttcctgggg atgctggctt ttgcggcgac cgcctggtgc
    14521 gaggcgccgg ctgcgggaaa tatcctgatt ggtcccggcg gtcgccaggc ccgcatcctg
    14581 gtttcgccac ccgcgcctct ggcccggctg atggccgggc gcctggccga ttacctggag
    14641 cagcgcaccg gcgttcggcc gcagctcatt tccgccctgc ccaaagaggg gaccgaaggc
    14701 gtggttttcc tgctggccca ggccgataac cgagccgtca gcaaactcgc cgcggactct
    14761 gcgcttgcgc tgcccgacat gaaagcctgg cgcgaggacg gctacgccct gcgcaccctt
    14821 gcccaaggca aaacccaata cgtcctctgc gccggcaagg gcgacagcgg cgtcaagtat
    14881 gcggtttacc gcc
//
LOCUS       DAOPCO010000073        14839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4080_length_14839_cov_5.273877, whole genome shotgun sequence.
ACCESSION   DAOPCO010000073 DAOPCO010000000
VERSION     DAOPCO010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14839
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4080_length_14839_cov_5.273877"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLS90_07105"
     CDS             complement(<1..231)
                     /locus_tag="PLS90_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02211.1"
                     /translation="MSKIGVGMIGCGGIAHYFGNYLKPHTDRIDFRAFCDVKPEATAR
                     FRADFAPDARVYADYRDLVRDPDIDWVLIASWN"
     gene            complement(377..1324)
                     /gene="rbsK"
                     /locus_tag="PLS90_07110"
     CDS             complement(377..1324)
                     /gene="rbsK"
                     /locus_tag="PLS90_07110"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPK02212.1"
                     /translation="MTPTESTRQAIVVVGSINIDLVARVPRRPDAGETLAADSFTQTP
                     GGKGANQAVAAARLGAPTWLVGRVGDDAFADPLRDNLRRHGVQLDHVLPTPDCSSGLA
                     LIHVDRSGENAITIVAGANARLTPADVDAAADRIARAAIVVLQLEVPLATAARAAQLA
                     REHGVPVLLDPAPAPGQPLLEPLRRVDFISPNQTEAATLTGERVESTEEAVQAARKLI
                     QAGAARVVLKLGSRGVLLLEGPDAEPVLVPAFNVDPVDTTGAGDAFTAGLAVGLAEGL
                     APADAARLGCAAGALATTRHGAQNAMPSREEADRLLQAR"
     gene            complement(1321..1932)
                     /locus_tag="PLS90_07115"
     CDS             complement(1321..1932)
                     /locus_tag="PLS90_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02213.1"
                     /translation="MHRLRLDQRLLWLACGLALLATAAEVLRVAQVRRHDAARRELAA
                     GRPLAAAAAVAGLRLFPYDPATHYTRLVILKQLDRWRALSDEAAAMRRWHPQQAGLLR
                     LKGEADWRLGRNQDAADALWDAFRFEPVPRDSPAQLWRIAMHAAADAAGAPSPRARAA
                     ARQVLALLDDDHRMTRQDRARARAEALRILAPEEAQGEGRDNP"
     gene            complement(1950..3605)
                     /locus_tag="PLS90_07120"
     CDS             complement(1950..3605)
                     /locus_tag="PLS90_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK02214.1"
                     /translation="MKAPRLLAVATVAACLGLAAPAPADVIVLNNGQTLRGIIDATTR
                     DESTVKIETAIGKLTVMRSRIKSIEQESPAQGYIHIGNDHRAKDQLSDALRAYQRAQQ
                     LEPANPQVQTLMDEVQREVTAREQLNRQGALQQIDELTRQARELIERQQFDEAEARLK
                     DADRLVPNDQQRATLQALVGEFYLAWARERLDKLDKAGAEEKLKLALGAQPDNQEIVD
                     ELLALWENDPQKREQAAAVYETILQRREDDANLRKKLADLYYELNQPADAVAHYLKLY
                     RDNPDYQGTDLEQRLVEMLDKLHKEQARQRNYAEAIRTFNVLAQLDPTADPTSVVFYE
                     YMRQAQELGDNDPARLLQLAQFAEQNGLDEEAMKHYNRLVTVDSTRAQAQAGIDRYAS
                     RALQQAQFYFNQGQWPLALTLAEQIIRDYPNSKAVVEEAVRLSGRAREEAARDLRQRE
                     VFAEAYVERGDEYFQQAQIHYNNIFSTERRDIPRLSSDKAEAKRYYQYAIDSYEEAIR
                     RNPNLQSDPRSLVMVRLRESREKIRRLSQPPTAGVRSFNRTIN"
     gene            complement(3693..5396)
                     /locus_tag="PLS90_07125"
     CDS             complement(3693..5396)
                     /locus_tag="PLS90_07125"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02215.1"
                     /translation="MASTAPVAFLPLFLRLKLRLANNHLSGLRRHLWIHLVVGLLVVF
                     MLIGGGSTLFHLVFRFLMEQQPFGPPLMDRLIRMVLLAFFSMLIFSNLIIMLTTTYIS
                     REVEFLMAQPIPHRRLFFAKLGESIVYSSWAFVILAFPLFVALRSSRDLPWTYFPSAA
                     CLLVPYLIIPAAAGAALALVIAAFFPPRRIIRFTLALGVLGMVMAVAMQRSQGVMGMM
                     RQASAEDTADLMSLMDVGDFFLLPSGWLGHGLVAIEHGFWREAGFWALALWSTAAMGL
                     VVCDWLAGLYYRGWTNARSSAAARRRRRAGLYGLFDRLLGWMPAATRAMVTKDLTVFW
                     RDPAQWGQLVMLFGLLFIYLINLRSAAGFGQFQIFIPFWQSIISLFNIGATAFVLSIL
                     TTRFVYPMLSLEGKQQWVIGLAPVARTRIVWEKFIVSWISAVGLTTPLALLSSFMLRT
                     GPFVTLLTVATVAVLAIGLSSMAVGLGALLPNFAEDNPSRIANGVGGTLNAILSLIYI
                     GATLALETIWVHAYLRRGDVGPLVQLLLWGSFPVWLLLQLGTAAIPLTLGLRRWRRME
                     F"
     gene            complement(5437..6171)
                     /locus_tag="PLS90_07130"
     CDS             complement(5437..6171)
                     /locus_tag="PLS90_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02216.1"
                     /translation="MIQTDQLCKDYDGKPAVVDLNLDVAPGELFCFLGPNGAGKTTTI
                     KMLAGLLIPTQGAARVAGFDLQRHPVEAKRRIGYIPDRPYLYEKLSGRDFFTFVGDLF
                     GIPRPVQQARLEHYFDLFRLTPAADQFIENYSHGMRQKLVFAAALMHEPQVLIVDEPM
                     VGLDPQSARTVKQVLREQADAGRTIFLSTHTLSVAEELADRIGVINKGQLVFLGTIRE
                     LRARLARDGSLEDLFLLLTEEETADV"
     gene            6441..6998
                     /locus_tag="PLS90_07135"
     CDS             6441..6998
                     /locus_tag="PLS90_07135"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPK02217.1"
                     /translation="MSVPPLIEHYLHRGQMRVEEHQHRPVYRARDLAAVECLAPATVA
                     KTVFFVCGRQMMLAVVPADRDLNLGHIRLLTGCRDARLATEREIRQRIETLAPGAVPP
                     FGSLFGMPVFMDDGLLDNDLFEVAGGDPARSLTIRMVDFLRQETPLILPLACAPAPER
                     TRRRAGRRSGAGMRVTEGAHAFVQA"
     gene            complement(7243..8715)
                     /locus_tag="PLS90_07140"
     CDS             complement(7243..8715)
                     /locus_tag="PLS90_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK02218.1"
                     /translation="MATATLPPRVRIGATQLLINNEWVKAASGKTFETINPATGDVIA
                     EVAEGDAADIDRAVKAARAAFERNSPWRRLAAAERGYLLNRLADLIEEHADELARLES
                     LDNGKPYEIAKAADLPLTIACYRYYAGWADKVHGKTIPIQGDYFCYTRHEPVGVVGQI
                     IPWNFPLLMQAWKLAPALACGNTVVLKVAEQTPLSALRVGELIIEAGFPAGTVNIVPG
                     YGPTAGAALARHMDVDKIAFTGSTEVGHLIMEAAAKSNLKRVTLELGGKSPNIVFADA
                     DLDAAIEGSHFALFFNQGQCCCAGSRLFVEEKVYDEFVERSTERARDRKVGDPFAEGV
                     EQGPQVDQEQFNKVMGYIDAGKQEGARMTCGGGRVGDRGYFIEPTVFADVNDDMKIAR
                     EEIFGPVMSLIKFKDLDEVVERANRTVYGLAAAVWTRDITKAHAIAANVRAGTVWVNC
                     YDVFDSAAPFGGFKQSGIGRELGEYGLQQYTEVKTVTIKI"
     gene            8959..9477
                     /locus_tag="PLS90_07145"
     CDS             8959..9477
                     /locus_tag="PLS90_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPK02219.1"
                     /translation="MDQSTSRIEVVGGDITRLDVDAIVNAANTSLLGGGGVDGAIHRA
                     AGPELLAECQTLGGCPTGEARITKGYRLPARHVIHTVGPVWRGGNRGEADLLASCYRN
                     SLPLAERHGLGTVAFPAISCGAYGYPVEAACRLAVRETRDWLKRHALPAKVIFVAFSE
                     ELEKAYREALGR"
     gene            complement(9506..11533)
                     /locus_tag="PLS90_07150"
     CDS             complement(9506..11533)
                     /locus_tag="PLS90_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPK02220.1"
                     /translation="MGKKLFKLFKDPAAKPDGPAKAGVVVPRGEEASATGSRMRDLIR
                     SHYSPEQVALLKNLIDSREGAAKYSDSPILVQDVIDPGADMLKIVSEEHGLRMIDLKR
                     YPRADYRLRGLIPAERAKALRVIPVEEKEDGTVVVAIADPSNPTVGDDLRLILGRDVE
                     TVIADESEIDERIEQYYGMGDESLEDIISQQETETVDEDVITTAENAIDLTDPESLGG
                     DQPIVQLVNLLLLRAIEDRASDIHIEPFPTFIRVRYRVDGVLREMPSPPRSQLVAIVS
                     RIKVMANLNISETRKPQDGRIKLLADGREIDMRISCMPTVHGEAVVMRVLDKSMMMIG
                     ISQIGMMGDVLDKFKKLIRRPNGILLVTGPTGSGKTTTLYAALAEVRDPGEKLITTED
                     PVEYEMDGIQQVNINESVGLTFARCLRAILRQDPDTVLVGEIRDVETAQIAVQAALTG
                     HLVFSTLHTNSAAATVTRLLDMGVEPFLITSAVEGVIGQRLVRTICQSCKTEVEPDED
                     ELADFGLTLKEVRDEGIRFFRGQGCEECNHTGYHGRLGIFELMEFTDEVRELVLERAT
                     TDEIHELAVRQGMITMRQDGWAKVTMGLTTMSEVLRQTPAEVITTAKSSSILVEREGE
                     GPVEGREALPSPQEALPAPNEALPAPGKPKLSAKSMIVENEEAASANAKNK"
     gene            complement(11593..12729)
                     /locus_tag="PLS90_07155"
     CDS             complement(11593..12729)
                     /locus_tag="PLS90_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPK02221.1"
                     /translation="MDINEMLKIVVDRDAADLHLRVGKPPVIRLHGEFEPLDPQPLTA
                     DDTERLMKEITPPRFQQQIQEQGSADFAYAFSEQARFRTNVFRTMGNIGIVMRLIPTR
                     IFTFDQLGLPKASIMPVLQAHRGMVIVTGPTGSGKTTSLATMIDWININRSVHIITIE
                     DPIEYTHPHKRAIVSHRELHIDTPSFANALRGALRQDPDVILVGEMRDLETMEAAITA
                     AETGHLVFGTLHTIGAATTVDRIIDQFPANQQEQIRSVLSVALSSVISQTLLPRASGK
                     GRVAAFEIMHNIAAVANLIREAKTNRLTSVIQTSAKLGMITLDDYLLRLYRKGIISGE
                     VCLERAQNYDEMRRNLMGEGIGGADAGDAKMEAAGATQAPVEEY"
     gene            complement(12789..14594)
                     /locus_tag="PLS90_07160"
     CDS             complement(12789..14594)
                     /locus_tag="PLS90_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPK02222.1"
                     /translation="MADPKPNGPRQSNGKKPVAPAGEAADRRPGDGKSLVTTQRGVQK
                     KGTDDEIKKWIKAVQGGKEAATRSRQELVSTDLLQTEGDILDAIAADTGMAKVVLSQV
                     NFTPELIAQVPAEVVRKYNVIPVDYNDREIWLAISDPHNIQALDDLSHVLGRKVHGRV
                     AREDDIERAIRAYYSKDEYRQLYDKLAESDDPGSLDQRYDELELGTEAAAEINQPPVV
                     KYVDLIFKQAVHERASDIHIEPTRVGVTIRFRIDGVLHEVPSPPRKWQNAIISRLKVL
                     SGMDLAEKRVPLDGRIRLNMPEKKLDLRVSSLPTIFGESIVMRLLDQSSVMMGLEDVG
                     FLPSTVKTFKKLIREPNGVILMTGPTGSGKTTTLYAALGVLNTPENKLVTLEDPVEYQ
                     IPGINQVQINHEIGLDFAYGLRSLLRQSPDIILVGEIRDLETAENAIRAALTGHLVFS
                     TLHTNDAPSSTIRLIDMGVKPYLVASSLQAAIAQRLVRRICGACKEPYAFDPEVVRDL
                     GYEAELEEGNLELFHGAGCERCSQSGYRGRTAIHEIMVMDSDLRRRVIRSEPASRLKK
                     AAVAKGMLTLRDDGWTKAKLGITTPEEVLRITAAD"
BASE COUNT         2359 a         4471 c         5415 g         2594 t
ORIGIN      
        1 gttccaggag gcgatcagga cccaatcgat atcgggatcg cggaccaggt cgcggtagtc
       61 cgcgtagacc cgggcgtcgg gggcgaagtc ggcgcggaag cgggcggtgg cctccggttt
      121 cacgtcgcag aaggcgcgga agtcgatccg gtcggtgtgc ggcttcaggt agttcccaaa
      181 gtaatgcgca atgccgccgc agccgatcat gccgacgccg atcttgctca tccgctcgcc
      241 cccccgtcca cgatgactcc agccgcacgg gcccatgatc ccgcaaagca tagggaaacg
      301 gggaggaagc ggcaaggccg aggtgaggat gacgaccggc gttcccttgg gcgggcgacg
      361 ggggctgcgc ggcacgtcac cgggcttgca gcagacggtc ggcttcctcg cgcgagggca
      421 tggcgttctg cgcgccgtgg cgcgtggtgg ccagggcgcc ggcggcgcag ccgaggcgcg
      481 cggcgtcggc cggggcgagg ccctcggcga ggccgacggc cagaccagcg gtgaaggcgt
      541 cgccggcgcc ggtggtatcg accggatcga cgttgaatgc cgggacgaga accggttcgg
      601 catccggccc ctcaagcaac aacacgccgc gcgagccgag tttcaggacg acgcgcgcgg
      661 cgccggcctg gatcagctta cgggcagcct ggaccgcctc ctcggtggat tcgacgcgct
      721 cgccggtcag cgtcgccgct tcggtctggt tggggctgat gaaatcgacg cggcgcaggg
      781 gctcgaggag cggttggccc ggagccgggg cgggatcgag gaggacgggg acgccgtgct
      841 cgcgggcgag ctgggcggcg cgcgccgccg tggcgagcgg gacctccagt tgcaggacga
      901 cgatggcggc gcgcgcgatc cggtcggcgg ccgcgtccac gtcggcgggg gtcaggcggg
      961 cgttggcgcc ggcgacgatc gtgatcgcgt tctcgccgga gcggtcgaca tggatcaggg
     1021 ccaggccgga cgagcagtcc ggcgtgggga gaacatggtc gagctggacg ccgtggcggc
     1081 gcaggttgtc gcggagcggg tcggcgaagg cgtcgtcgcc gacgcgtccg acgagccagg
     1141 taggggcgcc caggcgggcg gcggcgaccg cctgattggc gcccttgccg ccgggggtct
     1201 gcgtgaacga gtcggcggcg agcgtttcgc cggcgtcggg ccggcgcggc acgcgcgcga
     1261 ccagatcgat attgatcgaa cccacgacga cgatcgcctg gcgggttgat tcggtgggtg
     1321 tcacgggttg tccctcccct ccccctgtgc ttcttccgga gcgagaattc gcagcgcctc
     1381 ggcgcgggcg cgggcgcgat cctgccgggt catgcggtga tcgtcatcga gcagggcgag
     1441 cacctggcgc gcggcggcgc gggcgcgcgg gctgggggcg cccgctgcgt cggccgccgc
     1501 gtgcatggcg atccgccaca gttgcgccgg gctgtcgcgg ggaaccggtt cgaagcggaa
     1561 cgcgtcccag agggcatcgg cggcgtcctg gttgcggccg aggcgccagt cggcctcgcc
     1621 cttgaggcgc agcaggccgg cctgctgggg gtgccagcgg cgcatcgcgg cggcctcgtc
     1681 gctcagcgcg cgccagcgat cgagctgctt gaggatcacg agccgggtgt agtgcgtggc
     1741 gggatcgtag gggaacaggc ggaggccggc aacggcggcg gcggcggcca ggggccggcc
     1801 ggcggccaat tcgcgccgcg cggcatcgtg gcgccggacc tgggccacgc gcaagacttc
     1861 ggcggccgtc gccagcagcg ccagtccgca ggccaaccac aacaggcgct ggtcaaggcg
     1921 aaggcggtgc atcggccggc ccgatcggat caattgatcg tccggttgaa gctgcgcacg
     1981 ccggcggtgg gcggctggct cagccggcgg attttttcgc ggctttcccg cagccggacc
     2041 atcaccagcg agcgcggatc ggactggagg ttgggattgc gccgaatcgc ctcctcgtac
     2101 gagtcgatgg cgtactggta gtagcgcttg gcctcggcct tgtcgctcga caggcggggg
     2161 atgtcgcgcc gctcggtgga gaagatgttg ttgtaatgga tctgggcctg ctggaagtat
     2221 tcgtcgccgc gctcgacgta ggcttcggcg aagacctcgc gctggcgcag gtcgcgggcg
     2281 gcttcctcgc gcgcccggcc gctgaggcgg acggcttcct cgacgaccgc cttggaattc
     2341 gggtagtcgc ggatgatctg ctcggccagc gtcagggcga gcggccactg cccctgattg
     2401 aagtagaact gcgcctgctg cagcgcgcgc gaggcgtaac ggtcgatgcc ggcctgagcc
     2461 tgggcgcggg tcgagtcgac ggtcaccagg cggttgtagt gcttcatggc ttcctcgtcg
     2521 agtccgttct gctcggcgaa ctgcgccagt tgcagcaggc gggcggggtc gttgtcgccc
     2581 aactcctgcg cctggcgcat gtattcgtag aacaccaccg acgtcgggtc ggcggtcggg
     2641 tcgagctggg cgaggacgtt gaaggtccgg atcgcctcgg cgtagttgcg ctggcgcgcc
     2701 tgctccttgt ggagcttgtc gagcatctcc accaggcgct gttccagatc cgtgccctgg
     2761 tagtccgggt tgtcgcggta gagtttcaga tagtgggcga cggcatcggc cggttgattg
     2821 agttcatagt agagatcggc cagtttcttg cgcaggttgg cgtcgtcctc gcggcgctgg
     2881 aggatggttt cgtaaacggc ggcggcctgt tcgcgcttct ggggatcgtt ctcccagagc
     2941 gccagcaatt catcgacgat ttcctggttg tcgggttggg cgccgagggc cagcttgagt
     3001 ttttcctcgg cgccggcctt gtccagctta tccaggcgct cgcgggccca ggccagatag
     3061 aattcgccga ccagggcctg cagcgtcgcg cgctgctggt cgttggggac cagccggtcg
     3121 gcatccttca gccgggcctc ggcctcatcg aactgctggc gttcgatcag ttcgcgcgcc
     3181 tggcgcgtca gctcatcgat ctgctggagc gccccctggc gattgagctg ttcgcgggcg
     3241 gtcacctcgc gctggacttc gtccatcagc gtctggacct gggggttggc cggctccagt
     3301 tgctgcgcgc gctggtaggc gcgcagggca tcggagagct ggtccttcgc ccgatggtcg
     3361 ttgccgatgt ggatgtagcc ctgggcgggg gattcctgct cgatggactt gatgcgcgag
     3421 cgcatgacgg tcagcttgcc gatcgcggtt tcaatcttga cggtcgattc atcgcgggtc
     3481 gtggcgtcga tgatgccgcg cagggtctgg ccattgttga gcacgatgac gtcggccggg
     3541 gcgggcgcgg ccaggccgag gcaggccgcg acggtcgcga cggcgagcag gcgaggagct
     3601 ttcattgtcg attgccttcc taataaacgg gtcgccgagt gcttcatccc ctgatttgac
     3661 tcgatttatt gtagtccaac gcgacgcggg cgtcaaaact ccatccgccg ccagcggcgc
     3721 agccccagcg tcagcgggat cgcggcggtc cccaactgga gcagcagcca gaccgggaac
     3781 gatccccaga gcaacagttg gaccaatggg ccgacgtcgc cgcggcgcag gtaggcgtgg
     3841 acccagatcg tctccagggc gagcgtcgcc ccgatataga tcagactgag gatggcgttg
     3901 agtgttccac cgacgccgtt ggcgatgcgc gaggggttat cctcggcgaa gttgggcagg
     3961 agcgccccca gaccgaccgc catcgagctg aggccgatcg ccaggaccgc gacggtggcg
     4021 acggtcagca gcgtgacgaa cggcccggtc cgcagcatga agctggagag gagcgccagc
     4081 ggggtggtca ggccgacggc gctgatccaa ctgacgatga atttctccca gacgatgcgc
     4141 gtgcgcgcga cgggagccag gccgatcacc cactgctgct tgccctccag gctcagcatg
     4201 ggatagacga agcgcgtggt caggatggag aggacaaacg ccgtggcgcc gatgttgaac
     4261 agcgagatga tgctctgcca gaaggggatg aagatctgaa actggccgaa cccggcggcc
     4321 gaacgcaggt tgatcaggta gatgaagagc aggccgaaca gcatgacgag ctggccccac
     4381 tgggcgggat cgcgccagaa gacggtcaga tccttggtga ccatggcgcg ggtcgcggcg
     4441 ggcatccagc cgagcaggcg atcgaacagg ccgtagagtc cggcgcgccg gcggcggcgg
     4501 gcggcggccg aactgcgcgc gttggtccaa ccgcgataat aaagcccggc cagccagtcg
     4561 cagacgacca ggcccatggc ggcggtcgac cagagggcga gcgcccagaa gccggcctcg
     4621 cgccagaagc cgtgctcgat ggcgacgagg ccatggccca gccagccgct tggcagcagg
     4681 aagaaatcgc cgacgtccat caggctcatc agatcggcgg tgtcttcggc ggaggcctgg
     4741 cgcatcatgc ccatcacgcc ctgggagcgc tgcatcgcga cggccatgac catgcccagg
     4801 acgcccagcg cgagcgtgaa ccggatgatg cgccgggggg gaaaaaacgc ggcgatcacg
     4861 agggcgaggg cggcgccggc cgccgccggg atgatcaggt aggggaccag caggcaggcc
     4921 gccgagggga aataggtcca ggggaggtcg cgcgaggagc gcagggcgac gaagagcgga
     4981 aacgccagga tgacgaaggc ccaggagctg taaacgatcg attcgccgag cttggcgaag
     5041 aacagccgcc ggtgcgggat cggctgcgcc atcaggaatt ccacttccct ggagatgtag
     5101 gtggtcgtca gcatgatgat cagattggag aagatcagca tcgagaagaa cgcgagcagc
     5161 accatccgga tcagccggtc catcaacggc ggcccgaacg gctgctgctc catcaggaag
     5221 cggaaaacga ggtgaaacag ggtggagccg ccgccgatca gcatgaaaac gacgaggagc
     5281 cccacgacca ggtgaatcca cagatggcgg cggagacccg acagatgatt gttggccagg
     5341 cgcagcttga gccgcagaaa gagggggagg aaggcaacgg gggcggtgga ggccatggcg
     5401 ttgggtgatc cggcggagtc gggctcgggg cggacgtcag acgtcggcgg tctcctcttc
     5461 ggtcagcagg aggaacaggt cctcgagcga accgtcgcgc gcgaggcggg cgcgcagctc
     5521 gcggatcgtg ccgagaaaga cgagttggcc cttgttgatc acgccgatgc ggtcggccag
     5581 ttcctccgcg accgacagcg tatgcgtgga gaggaagatg gtgcggccgg cgtcggcctg
     5641 ctcgcgcagg acctgcttga cggtgcgggc cgactgggga tcgagcccga ccatcggttc
     5701 atcgacgatc agcacctgcg gctcgtgcat cagcgcggcg gcgaagacca gcttctgacg
     5761 catgccgtgc gagtaattct cgatgaattg atccgccgcg ggcgtcagcc ggaagaggtc
     5821 gaagtagtgt tccaggcggg cctgctgcac ggggcggggg atgccgaaga gatcgccgac
     5881 gaacgtgaag aagtcgcggc cgctgagttt ttcatagagg taggggcggt cggggatgta
     5941 gccgatgcgg cgcttggcct cgacgggatg gcgctgcaag tcgaagccgg cgacgcgcgc
     6001 ggcgccctgg gtcgggatga gcaggccggc cagcattttg atcgtggtgg ttttgcccgc
     6061 gccgttggga ccgaggaaac agaacagttc gccgggagca acgtccagat tcagatcaac
     6121 caccgccggt ttcccgtcgt aatccttgca caactggtcg gtctggatca tcaaatgggt
     6181 ccacctcggg gagtctgggg gcggggcggg cgcgccgtgc ggcgcgcggg gccctcctga
     6241 aataccatac ctgcaatggt tgcagccatc ggcgagttgg agtggcgggg ttgccggtat
     6301 gcgaatgggg gaaatcccga ctcgctcggc gattgcgcgg gaggcgtcat cggcgcaata
     6361 atgaccatgc gcaagtcgat cgagttaccc gcatttgagt tcgaaaaaat tcttccgcca
     6421 tccgagggag gtgaagcgcc gtgtcagtcc ccccgctgat cgagcactat cttcaccgcg
     6481 gccagatgcg agtggaggaa caccagcacc ggccggttta tcgcgcgcgc gacctggcgg
     6541 ccgtggaatg cctggccccg gcgacggttg cgaaaacggt gttttttgtc tgtggccggc
     6601 agatgatgct ggcggtggtg cccgccgacc gcgatttgaa cctgggccac atccgcctgc
     6661 tgaccggctg ccgcgatgcc cgcctggcga ccgaacggga gatccgccag cggattgaaa
     6721 cgctggcgcc gggggcggtc ccgcccttcg gctcgctgtt cgggatgccg gtcttcatgg
     6781 atgacggcct gctcgacaac gatctgttcg aagtggccgg cggagatccg gcccggtcgc
     6841 tgacgatccg aatggtcgat tttctgcgcc aggagacgcc gctgatcctg ccgctggcct
     6901 gcgcgccggc accggagcga acgcggcggc gcgccggacg gcgcagcggc gccgggatgc
     6961 gcgtgaccga gggcgcccat gccttcgtgc aggcgtaggc gggccgggca taggatgtaa
     7021 gcgggtagcc ccccaagtga gcgaaatccg tcggatccga cggatttcga gtcgcgacgg
     7081 attttcctta cgtcccttat atcccttgtg tccctgctct tcctcgatca cgaggaacga
     7141 gagacgaggg aggattcgtg cgccccgggg ggacaaaacc cctgcggtcc gggtggggga
     7201 aggagcgtcg cgaaccgcag gggctgtccg tgggaggaac gttcagatct tgatcgtcac
     7261 cgtcttgact tcggtgtatt gctggaggcc atactcgccc agttcgcggc cgatgccgga
     7321 ctgcttgaac ccgccgaacg gggcggccga atcgaagacg tcgtagcaat tgacccagac
     7381 cgtgccggcg cggacgttgg cggcgatggc gtgggccttg gtgatgtcgc gggtccagac
     7441 ggcggcggcc aggccgtaga ccgtgcggtt ggcgcgctcg acgacctcat cgaggtcctt
     7501 gaacttgatg aggctcatca ccgggccgaa gatctcctcg cgggcgatct tcatgtcgtc
     7561 gttcacatcg gcgaagaccg tcggctcgat gaagtagccg cgatcgccga cgcggccgcc
     7621 gccacaggtc atccgcgcgc cttcctgctt gccggcgtcg atgtagccca ttaccttgtt
     7681 gaattgctcc tggtccacct gcggtccctg ctcgacgccc tcggcgaagg gatcgccgac
     7741 cttgcggtcg cgggcgcgct cggtgctgcg ctcgacgaac tcgtcgtaga ccttctcctc
     7801 gacgaacagg cgcgaacccg cgcagcagca ctgcccctgg ttgaagaaga gggcgaaatg
     7861 ggagccctcg atggcggcgt ccagatcggc gtcggcgaag acgatgttcg ggctcttgcc
     7921 gcccagctcg agcgtcacgc gtttcaagtt gctcttggcg gcggcctcca tgatcaggtg
     7981 gccgacttcg gtcgagccgg tgaaggcgat cttgtcgacg tccatgtggc gggcgagggc
     8041 ggcgccggcg gtcggtccgt agccggggac gatgttgacc gtgccggcgg ggaacccggc
     8101 ctcgatgatc agctcgccga cgcgcagggc agaaagcggg gtctgttcgg ccaccttcag
     8161 gacgacggtg ttgccgcagg ccagggccgg agcgagcttc caggcctgca tcagcagggg
     8221 gaagttccag gggatgatct ggccgacgac gccgaccggt tcgtggcgcg tgtagcagaa
     8281 gtaatcgccc tgaatcggaa tcgtcttgcc gtggaccttg tcggcccagc cggcgtagta
     8341 gcggtagcag gcgatggtca gcgggaggtc ggccgccttg gcgatctcgt agggcttgcc
     8401 gttgtcgagg ctttcaaggc gggccagttc atcggcgtgt tcctcgatga ggtcggccag
     8461 gcggttgagc aggtagccgc gctcggccgc ggcgagccgg cgccaggggc tgttgcgctc
     8521 gaaggcggcg cgggcggcct tgacggcccg gtcgatatcg gccgcatcac cctcggcgac
     8581 ctcggcgatc acgtcgccgg tggcggggtt gattgtctcg aaggtcttgc ccgaggcggc
     8641 cttgacccat tcattattga taagaagctg cgtggcgccg atgcgcaccc gcggcggcag
     8701 tgtggcggtt gccatgatga gggactcctt tgactggcag aaagcgagct cgctcggccc
     8761 cggaggaggc acggatgcac gggacgtgat acgatagcaa acgctggagg ggatacgatt
     8821 tgcccgacgt atcccgaggc tcaaatgatt acagataaat ctactattgc gcgggcgaag
     8881 gatcaaccgt tttcgtgggt gggggccggg tgcgacgggg aggatgcggg agtgaaaagc
     8941 caatggaggt gaccggccat ggatcaatca acctcgcgga ttgaggtcgt cggcggcgat
     9001 atcacccggc tcgacgtaga tgccatcgtc aatgccgcca acacgtcgct gctgggcggc
     9061 gggggcgtcg acggggcgat tcaccgcgcg gccggacccg aactgctcgc cgagtgccag
     9121 acgctggggg gctgccccac cggcgaggcg cgcatcacga agggctatcg ccttccggcg
     9181 cgccatgtca ttcacaccgt ggggccggtc tggcgcggcg gcaaccgcgg cgaggcggat
     9241 ctcctggcga gctgttaccg caacagcctg ccgctggccg aacgccacgg gctcgggacg
     9301 gtggcgttcc ccgcgatcag ttgcggcgcc tacggctatc cggtcgaggc ggcctgccga
     9361 ctggcggtgc gggagacccg cgactggctg aaacggcacg cgctgcccgc gaaggtgatc
     9421 ttcgtcgcct tcagcgaaga gctggagaaa gcctaccggg aagcattggg gagataaaag
     9481 aattcgggcg cgatcatccg gcgcgctact tgttcttcgc gttggcgctg gcggcttcct
     9541 cgttctcgac gatcatcgac ttggcgctga gcttcggctt gcccggcgcg gggagggcct
     9601 cgttcggggc cggcagggct tcctggggac tgggcaaagc ctcgcgccct tcgaccgggc
     9661 cctcgccttc gcgctccacc aggatcgagc tggacttggc cgtcgtgatg acctcggcgg
     9721 gggtctggcg caagacttcg ctcatcgtcg tcaggcccat cgtgaccttg gcccagccat
     9781 cctggcgcat ggtgatcatc ccctggcgca ccgccagttc gtggatctcg tcggtggtgg
     9841 cgcgctcgag caccagctcg cgcacctcgt cggtgaattc catcagctcg aaaatgccga
     9901 gccggccgtg gtagccggtg tgattgcatt cctcgcaccc ctggccgcgg aagaagcgga
     9961 tcccctcgtc gcgcacctcc ttgagcgtca gcccgaaatc ggccagttcg tcctcatcgg
    10021 gttcgacttc ggtcttgcac gactggcaga tcgtccggac gagacgctgg ccgataaccc
    10081 cttccaccgc cgaggtgatc aggaacggct cgacgcccat gtcgagcaga cgcgtcacgg
    10141 tggcggcggc cgagttggtg tgcagcgtgg agaagacgag gtgaccggtc agcgcggcct
    10201 ggacggcgat ctgggcggtc tcgacgtcgc ggatttcgcc gacgaggacg gtgtcggggt
    10261 cctggcgcag aatcgcgcgc aaacagcggg cgaaggtcag gccgacgctt tcgttgatgt
    10321 tgacctgctg gatgccgtcc atctcgtact cgaccgggtc ttcggtcgtg atcagcttct
    10381 cgccggggtc gcgcacctcg gccagggcgg cgtagagcgt cgtcgtcttg cccgagccgg
    10441 tggggccggt gacgagcagg atgccgttgg ggcggcggat cagtttcttg aacttatcca
    10501 gcacgtcgcc catcatgccg atctggctga tgccgatcat catcatgctc ttgtcgagca
    10561 cgcgcatcac gacggcctcg ccgtggacgg tcggcatgca actgatccgc atgtcgatct
    10621 cgcggccgtc ggcgagcagc ttgatgcggc cgtcctgcgg cttgcgggtc tccgagatgt
    10681 taaggttggc catgaccttg atgcgcgaga cgatggcgac caactgggag cgcgggggcg
    10741 agggcatctc gcgcaggacg ccgtcgacgc ggtagcggac gcggatgaac gtgggaaacg
    10801 gttcgatgtg gatgtcgctg gcgcggtcct cgatggcgcg cagcagcagc aggttgacca
    10861 actggacgat cggctgatcg ccgccgagcg attcgggatc ggtcaggtcg atggcgtttt
    10921 ccgccgtcgt gatgacgtcc tcgtcgaccg tctcggtctc ctgctggctg atgatgtcct
    10981 cgagcgactc atcgcccatg ccatagtatt gttcgatgcg ctcgtcgatt tcggattcgt
    11041 cggcgatgac ggtttcgacg tcgcggccga ggatgaggcg caggtcatcg ccgacggtcg
    11101 ggttggaggg gtcggcgatg gcgacaacga ccgtcccatc ctctttttcc tcgacgggga
    11161 tgacgcgcag ggccttggcg cgctcggcgg ggatcaggcc gcgcaggcga taatcggcgc
    11221 gcgggtagcg cttcagatcg atcatgcgca gaccgtgctc ctcggagacg atcttgagca
    11281 tgtcggcgcc gggatcgatg acatcctgga cgaggatcgg cgaatcgctg tatttggcgg
    11341 cgccttcgcg cgaatcgatg aggttcttga gcagggcgac ctgctccggc gagtaatggg
    11401 agcggatcag atcgcgcatg cggctgccgg tggcgctggc ctcctcgccg cggggcacga
    11461 cgacgccggc tttggccggg ccgtcgggct tggcggcggg atccttgaag agcttgaaca
    11521 gcttttttcc catggtcttc ttccgatgct ccagggttgg ccgcgcggcg tgcgccggca
    11581 acgcgggctg gctcaatatt cctccacggg ggcctgggtc gcgccggcgg cctccatctt
    11641 ggcatcgccg gcgtcggcgc cgccgatccc ctcgcccatc aggttgcggc gcatttcatc
    11701 gtagttctgc gcccgttcca ggcagacctc acccgaaatg atccccttgc gatacaggcg
    11761 cagcagatag tcgtcgagcg tgatcatgcc gagcttggcg ctggtctgaa tgacggaggt
    11821 caggcggttg gtcttggctt cgcggatcag gttggcgacg gcggcgatgt tgtgcatgat
    11881 ctcgaacgcg gcgacgcgcc ccttgcccga ggcgcgcggc agcagcgtct gcgaaatgac
    11941 gctggagagc gcgaccgaga gaaccgagcg gatctgctcc tgctggttgg cggggaactg
    12001 gtcgatgatg cggtcgacgg tggtggcggc gccgatcgtg tgcagggtac cgaagacgag
    12061 gtggccggtc tcggcggcgg tgatggccgc ctccatcgtc tccaggtcgc gcatttcgcc
    12121 gacgagaatg acgtcggggt cctggcgcag ggcaccgcgc agggcattgg cgaacgacgg
    12181 cgtgtcgatg tgcagctcgc ggtgggaaac gatggcccgc ttatgcggat gggtgtactc
    12241 gatcgggtcc tcgatggtga tgatatgaac gctgcggttg atgttgatcc agtcgatcat
    12301 cgtcgccagc gaggtggtct tgcccgaacc ggtggggccg gtgacgatca ccatgccgcg
    12361 gtgggcctgc agtaccggca tgatcgaagc tttgggcagg ccgagctgat cgaaggtgaa
    12421 gatgcgggtc gggatcaacc gcatgacgat gccgatattg cccatcgtgc ggaagacatt
    12481 ggtacggaag cgggcctgct cgctgaaggc gtaggcgaag tcggcgctgc cctgctcctg
    12541 aatctgctgc tggaagcggg ggggcgtgat ctccttcatc aggcgttcgg tgtcgtcggc
    12601 ggtcaggggc tggggatcaa gcggttcgaa ctcgccatga agccggatga cggggggctt
    12661 gcccacgcgc aggtgcaggt cggcggcgtc gcggtcgacc acgatcttga gcatttcgtt
    12721 gatatccatc gctgcgtcct gttccagatg ctgtcgtgcg gtgggagccc ccggggagcc
    12781 gacgggcgtc agtcggcggc ggtgatccgc aggacctcct cgggggtggt gatgcccagc
    12841 ttggccttgg tccagccgtc gtcgcgcagg gtcagcatgc ccttggcgac cgcggctttc
    12901 ttgaggcgcg aggcgggttc gctgcggatg acccggcggc gcagatccga atccatcacc
    12961 atgatctcgt ggatcgccgt ccggccgcgg tagccgctct ggctgcaccg ttcgcagccg
    13021 gccccgtgaa acagctccag attgccctcc tccagttcgg cttcgtagcc caggtcgcgg
    13081 acgacctccg gatcgaaggc gtagggctcc ttgcaggcgc cgcagatgcg gcgcacgagg
    13141 cgctgggcga tggccgcctg caacgaactg gccacgaggt acggcttgac gcccatgtcg
    13201 atcagtcgaa tggtcgaact gggcgcgtcg ttggtatgga gcgtcgagaa gacgaggtga
    13261 ccggtcagcg ccgcgcggat cgcgttttcg gcggtttcca ggtcgcggat ctcgccgacc
    13321 aggatgatgt cgggcgactg gcgcagcaac gagcgcagcc cgtaggcgaa gtccagcccg
    13381 atctcatggt tgatctggac ctgattgatg ccggggatct ggtattcgac cgggtcctca
    13441 agcgtgacca gcttgttctc cggggtgttg agcacgccca gcgcggcgta gagcgtcgtc
    13501 gtcttgcccg agccggtcgg cccggtcatc aggatgacgc cgttgggttc gcggatcagc
    13561 ttcttgaaag tcttgacggt gctgggcagg aagccgacgt cctccaggcc catcatgacc
    13621 gaggattgat cgagcagacg catgacgatc gattcgccga agatggtcgg caggctcgag
    13681 acgcgcaggt cgagcttctt ttccggcatg ttgaggcgga tgcggccgtc cagcgggacg
    13741 cgcttttcgg ccagatccat gcccgaaagg accttcaggc gcgagatgat cgcgttctgc
    13801 cacttgcgcg gcggactggg gacctcgtgc agcacgccgt cgatgcggaa gcggatggtc
    13861 acaccgacgc gggtcggttc gatgtggatg tcgctggcgc gctcgtgaac ggcctgcttg
    13921 aagatgaggt cgacatattt gacgacgggc ggctggttga tttcggccgc ggcctcggtg
    13981 cccagctcca gctcgtcgta gcgctgatcg agcgagccgg gatcgtcgct ctcggccagt
    14041 ttgtcgtaga gctggcgata ttcgtccttg ctgtagtaag cgcggatcgc ccgctcgata
    14101 tcgtcctcgc gcgccacccg gccgtggacc ttgcggccaa ggacatggct caggtcgtcg
    14161 agcgcctgga tattgtgcgg gtcggagatg gccagccaga tctcgcgatc attgtagtcg
    14221 accgggatga cattgtattt gcggacgact tcggccggga cctgggcgat cagttcgggg
    14281 gtgaaattga cctgcgagag gaccaccttg gccatcccgg tgtcggcggc gatggcatcg
    14341 aggatgtcgc cctcggtctg gagcagatcg gtgctgacca attcctggcg cgagcgggtg
    14401 gcggcttcct tgcccccctg gaccgccttg atccatttct tgatctcgtc gtcggttccc
    14461 tttttctgaa cgccgcgctg agtcgtgacg agactcttgc catcgccggg gcgccgatcg
    14521 gcggcttcgc cggcgggcgc gaccggcttc ttgccgttgg attgcctggg gccattgggt
    14581 ttcggatcgg ccatggtcaa tgcctctgac tcggattacg gaattcagcg tcgccgcagc
    14641 gggcaaacgc cgacgccatc gcgcagtttc cctccggcga cagacccggc gggactcgcg
    14701 atcattccga agtgcgggac acggaggtgc tgaagaaaac gggccgccga ctgggaaagc
    14761 cgaacgacgg aggtctctcc agcgccccgg cccagtttca tggtgaattt tactttatgc
    14821 atcaagaaaa agtcaacct
//
LOCUS       DAOPCO010000074        14529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4199_length_14529_cov_4.452812, whole genome shotgun sequence.
ACCESSION   DAOPCO010000074 DAOPCO010000000
VERSION     DAOPCO010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_14529_cov_4.452812"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..1336)
                     /locus_tag="PLS90_07165"
     CDS             complement(329..1336)
                     /locus_tag="PLS90_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HPK02223.1"
                     /translation="MAHTICLIPGDGIGPEVTRAAQAVLEAAGVAIDWLTLPAGATAA
                     EEYGDVLPAKTIEAIEHYRLALKGPVTTPVGKGFSSVNVQLRKRFALYAAVRPVRSMP
                     GIKTRFENVEIVVIRENTEGLYSGIENEIVPGVVQSLKIATRPACERIARFAFKYARE
                     RERRKVTVFHKANIMKKSDGLFLECAQQAHAEMGQGITFQQLIIDNGCMQLVRDPTQF
                     DVLLLENLYGDMISDLCAGLVGGLGVVPGANIGDDCAIFEAVHGSAPDIAGRGLANPL
                     ALIMSSVMMLNYIHELEAAERIKAAYNEILLEHNPENLTADIGGTGGTEDFVRAVIAR
                     L"
     gene            1729..2679
                     /locus_tag="PLS90_07170"
     CDS             1729..2679
                     /locus_tag="PLS90_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02224.1"
                     /translation="MTIRWSVIPLCLMTAAVTAQPPVQEVFPATPADGAVSKLTEDEV
                     IHGLSDEPGDPGHSTIDRELEAAREAYIAEDKDSSLQHLTNALVILSHEAAWSAGEYE
                     ATLREAIAELEKLAHEVQEGQIERDQIGDRFKDAYSKVYAGLSRYHLSRAEGMVRERQ
                     TREGIVRNDQRATETESAAVATPAPLAIDVETTPAQTTDDDEHWRVELGRQISGATEN
                     VRAWSRETGREIRDEPVIAELQEIAGRIIAGTGWATEEVGEALNRFGEKIKQYGRDQT
                     DQGRTKQLSDPDEAADERAEMEREAAKRARDAAEEAAEDH"
     gene            complement(2790..2996)
                     /locus_tag="PLS90_07175"
     CDS             complement(2790..2996)
                     /locus_tag="PLS90_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02225.1"
                     /translation="MSKPVKIVVIVVLLVAASFIFYWQLKAKNLIGRPEAPAPGAPGM
                     PGMPPGFAPPPGTTPMPPAMPPAE"
     gene            3295..4494
                     /locus_tag="PLS90_07180"
     CDS             3295..4494
                     /locus_tag="PLS90_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02226.1"
                     /translation="MQRQPPPSPARPTPRPLSRRRKAAFALAAALLLLLALEAGARLW
                     LVYDRHLPAARPDLAALTYYPELRPLLLADPPPGADTCDVLLLGGSVLFEYYGGPALH
                     LEAALEQRLHRPVRVWNLGRPSHASRDSLIKYRWLDSLRFDAVVVYHAINELRTNHAP
                     ADRFRLDYDHVPWYREVNALAGHPAGGFSRACALPLLVRYAPLWLERQLRPRGPLAPD
                     AMPGPEDLIHGDDIKSTASLAANLSEIAAIARRRGDPLLLLTYAWWLPDGYSDEDLLH
                     DRELAIARAEARPPTVDGPRRLPFADPLYADFAQPVSIWGRREQLVKGLSLHNEVIRA
                     VARDHAIPLVDLAARMGDSGDYFIDICHLSPAGVARLVDGILDGLTLLSFPAAPGDPA
                     RQPAHST"
     gene            4986..5714
                     /locus_tag="PLS90_07185"
     CDS             4986..5714
                     /locus_tag="PLS90_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPK02227.1"
                     /translation="MNPSRLRIHLLAPVALACMLAGCGPSVEKSIAFQEQVSRTDSVE
                     ESLKDLSRQLSMLDTEFQQVSRMVAEGQGGAGGADSNATKALEARVLRLEQAFQESQK
                     TLTAIQNALEEGKGVRIASAARVAAAPPAEPAQQPQEVNEQVTTAKLKVVGKGSSRAV
                     AAPAPAPSRPAAQPRPAVRVEQPEPREAARRSGIYHKVTQGQSIDQIASHYKTSVGAI
                     RQANHLPERARLIAGQQIFVPTGR"
     gene            complement(5737..7242)
                     /gene="argH"
                     /locus_tag="PLS90_07190"
     CDS             complement(5737..7242)
                     /gene="argH"
                     /locus_tag="PLS90_07190"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPK02228.1"
                     /translation="MSSPPASGPIWSKRTSGGPSAAVLAYCSGRDVKGRPAADEQLIP
                     YDLWTNRAHCLMLQRRRIIDAGTCRLILEELERIEEAWHAGRLRLDPALEDVHMNLER
                     LVARRDEAAAGVMHTARSRNDQSATDVRLWLRDRLLEQTLATAELVQTLGRLARAHAE
                     TIAPGWTHGQPAMPTTLGHWAAAHGFALTRDLAALRALWPLINVSPLGAAASFGTSWP
                     IDRRLSARLLAFDAPQANSLDAISTRWEAEARLGGVLAILMSHLASLGQDLIFLSTPP
                     RVGLRLADAHVSGSSIMPNKRNPDFAEVTRARALAVQGLAANLAGVGRGLLTGYNRDT
                     QWTKYWVMDLVEEVGEAPRVFAEVLGGLRADRPVLRELATAGFALAADLADHLARTRH
                     LPFRQAYHVVAEAVARDEARGWISAATLNEILRRAGIDPPLTPEELDAAGRPEQALAR
                     RGSLGGPEPGDVRGQAAALGQLAGEARQWARAQARRIAAARDRARRTRPRD"
     gene            7644..8474
                     /locus_tag="PLS90_07195"
     CDS             7644..8474
                     /locus_tag="PLS90_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02229.1"
                     /translation="MSRNRCKWAMGAALVIACVLAGGCATGGESEPQPEDLTLLVTLN
                     KSVYRPGEIVEARVKLANNTNEQLELPKLSAADGGSISFWYSLATEPDASMQRPPVVS
                     RLERPEGLITLAPGQSEERTFVLTRLAEYSGPMLLQVHYDPNPAGSGRTEPKIFSNPV
                     AYDVKGELLFGRDPSGYLMEEEAIGLANAVAPGEVRSGDAILIEDEMGFYKWWVNLQV
                     QREGGQTQTLAWLVDPYFGRVIGQAQPFPENMQNDPRTRGQKSLRRDPSRPDRVYMED
                     "
     gene            8781..10439
                     /locus_tag="PLS90_07200"
     CDS             8781..10439
                     /locus_tag="PLS90_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02230.1"
                     /translation="MYKRLAWLWAAGWLVATSLATAATVDETRHLPPNDYPSHTVKIL
                     RTTNKAQTQSYVPVVFTMRNNNPFNVIQYLHRPIQHEEGLLFTFVSPEGNGGHVLYCV
                     PEYMIESLGRLVAALDRPGLTTSAGTTRIYRQLKHRRASATDPGFIETAASFSTGNGN
                     DFLIDPWANALYYEDADSGAMALDNALTDWLDVPTAMVDVMVKVYELAAYNDATIGLD
                     YISWKNGPGRDLFAVGLFSEYASVERAGSNLSVFPPNADPSALGLRQGLSTRNNLSAS
                     GWNYAWSYNVDSSFFDYLQTKGKARVLNRARLSMLNSWPAYLDSGDQVMSYAVGTSNP
                     SGIRDSGEIFQANTGRLMTGTTNQIVTREPEDLEQLILNLVLNGFDSGLSGLSPYSYC
                     LEPIETGFFLALDNVIGEQSVTLEVDMEWSDYTAFDDTGFPQIQCRKLHIEDLRLALG
                     DEIIIGGLKRQAHAVATQKVPFFGSLPIVGYLFGGEDNLNKQTELVVAIKPVEITSYQ
                     IARDDQAVIDKATGQAEIVEPDTTMGFDQYIFDKARDVEAMPFN"
     gene            10502..>11403
                     /locus_tag="PLS90_07205"
     CDS             10502..>11403
                     /locus_tag="PLS90_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02231.1"
                     /translation="MTTKWLTRSMRVLGVFGLSAGLTWAAYPEFENVQRLNADPSPTK
                     SESNINEYTYQDEQINIVPSDGVVKVLRTDQKILINDYVTAVIPIKHAEPRELRNVFR
                     QVTALEGGRAEVIRDKETGENFLQVIAPEYMIPYLRDAVAALDDSWVEEYNDGSSDLY
                     LNMQHRDAAEVDFFAANYAGNDGFSTIDTTNNSVRRYDEIYRNEKYAAAATLFDIPCN
                     QVELEIKVYEVNTSNDLKLGLDYINWQNGPGRNLWYFVQSGYSAEQRAFGRTSVFDPF
                     LDARAPIHPGVTDKTLVQDTAARAG"
     assembly_gap    11404..11413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11414..12396
                     /locus_tag="PLS90_07210"
     CDS             <11414..12396
                     /locus_tag="PLS90_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02232.1"
                     /translation="NYLLTSNFIDFLQVKGKARAIDHQTITLCSGSSGSIGTLDQVLA
                     FVDNMNDITDFADENPTYIRRQNNGIGVPDSTPNLLAKTGFGDGLDQFFLDVNGNGIR
                     DFIDENNNGVRDFIDLNNDGIRQENEPFTEPLEDLLGDSPSSVRVQDSTRRLHYENAG
                     TVGMKVCVTPYVGLESMELDIDLDIGEMNGIAPNGTPIINFRSLATTVRLMDGQPFVL
                     AALKQVNDVKSTAKAPFLGSIPVVGYLFGGETDVKRENDVVITITPHFQLSDQVKLYK
                     PPRIDTLQMIIDGTLPQGAPAIDYGYDQWLLDCGHKDHAAANAGADEDMS"
     gene            12549..12776
                     /locus_tag="PLS90_07215"
     CDS             12549..12776
                     /locus_tag="PLS90_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02233.1"
                     /translation="MGSPREEKPRRTPARRIDGDHRRTGRADEPSNPARSGNNRTASH
                     VENPVRDEEDPRIKSKPGQPLHSKKEDMDHV"
     gene            complement(12805..13215)
                     /locus_tag="PLS90_07220"
     CDS             complement(12805..13215)
                     /locus_tag="PLS90_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPK02234.1"
                     /translation="MSTSNSAGNSSKAEEGGEAATAVAQTGDGWRRLVPRRQDGVEAR
                     SEGGERVQLRRRRPARGGVRGWLDRKLGRSVFIQVELDARGAFFWGLINGIDTLETIE
                     ERMRERFGFEREASREATLRFALLLARRGLIGLG"
     gene            complement(13175..14146)
                     /locus_tag="PLS90_07225"
     CDS             complement(13175..14146)
                     /locus_tag="PLS90_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK02235.1"
                     /translation="MTRRRLLASAGLTTAAATLIGCQGTWSSAKGQSGGTGARRSLRL
                     AHLTDVHVQPEKRAPEGLAACLRHAQGLADPPQLILNGGDCILDCFTTDAERARTQWE
                     VWQRVLAAECHLPVIHCLGNHDIWGWDKASARTTGDEERFGKRWALEEFGVERHYRSF
                     DRDGWHFIVLDSTSSDGGNYYFARLDDEQFAWLEEDLARLAGARPVVVLSHIPILSAA
                     SFFDGENEKPGDWVVPRAWMHIDARRIKDLFLKHPNVRLCLSGHLHLVDRVEYCGVTY
                     ICDGAVCAGWWDGPYQECWPGYGVVDLYEDGSFSHEYVQFGWEFEQS"
BASE COUNT         2507 a         5624 c         4111 g         2277 t
ORIGIN      
        1 ccccgccgtt caagcaaaaa agatgagctg ctggacgaaa atatgccccc acgccccgct
       61 ccgaccccgc agccggcacg tcctcccccg cggcctcgcc gcctcagtgg aaaacccaac
      121 cgcccgaaac ctgggagcgc cacgctcctg agtggcagtt tgtgaaccgc cgattcgccc
      181 ccaaagcccg cctcggaatt cggctctcgt ccctcgtcct cggctctcga aatcgagccg
      241 tccaccagat caaccagcgc ctgcgaccgc cggcatcctg gccggcacaa gcccccgccg
      301 tgctatcatc gcctctctca tccgcccctc acagccgcgc gatcaccgcc cgcacgaaat
      361 cctccgtccc ccccgtcccg ccgatgtccg ccgtcaggtt ctccggatta tgctccagca
      421 gaatctcgtt atacgccgcc ttgatccgtt cggccgcttc cagctcgtgg atgtaattca
      481 acatcatcac gctgctcatg atcagcgcca gcggattggc cagcccgcga cccgcaatgt
      541 ccggcgcgct gccgtgaacc gcctcgaaaa tcgcgcaatc gtcgccgata ttcgcccccg
      601 gcaccacccc caggcccccc accagccccg cgcacaggtc gctgatcatg tcgccgtaga
      661 gattctccag cagcagcacg tcgaactgcg tcgggtcgcg caccagctgc atgcagccgt
      721 tgtcgatgat cagttgttga aacgtgatcc cctggcccat ctccgcatgc gcctgctgcg
      781 cgcattccag aaacagcccg tcgctcttct tcatgatgtt cgccttgtgg aacaccgtca
      841 ccttcctgcg ctcgcgttcg cgcgcatatt tgaatgcgaa tcgcgcgatc cgctcgcacg
      901 ccggccgcgt ggcgatcttc aagctctgca ccacccccgg cacgatctcg ttctcgatcc
      961 ccgaatacag cccctcggtg ttctcccgga tgacgacgat ctctacattc tcaaagcgcg
     1021 tcttgatccc cggcatcgac cggaccggac gcaccgccgc atacagcgca aaccgtttgc
     1081 gcagctgcac gttgacgctt gaaaacccct tccccaccgg cgtcgtcacc gggcccttca
     1141 gcgccagccg gtaatgctcg atcgcctcga tcgtcttggc cggcagcacg tcgccatatt
     1201 cctcggccgc cgtcgcgccc gccggcaacg tcagccagtc gatcgcgacc cccgccgcct
     1261 ccagcacggc ctgcgccgcg cgcgtgactt ccggtccgat gccatcgccg gggatgagac
     1321 agatcgtgtg agccatgcgg gtatccttgg cgcgtcagta ggctgggcca tgcggccggc
     1381 ggccgcgctc accccgaccg ggctttgggt cggggattga gatctttggg gtcggagaat
     1441 atggcctccg ggccgctgcc tcagcatggc aactcagccc cgcattgtca aagcccaccc
     1501 cgccccctca aaaaatccgg ctctcggcgc tcgtccctcg tcctcggctc tcgaaaaccg
     1561 ctcctttgcc ccatcatcgc cttctgcctc ctgcccctcg cccgccttat cttctggcac
     1621 gcgacatgca tcaaccctta cccgcaccct gcttccttcg ggaacgcggg gcggactcgc
     1681 cgcgtccatg cccggccagc cgatgtccca cgatgaggtg tcccaaccat gacaatccga
     1741 tggagcgtga ttccactctg cctgatgacg gccgcagtca ctgcccagcc gcccgttcag
     1801 gaggttttcc ccgccacccc ggccgacggg gcggtatcca agctgaccga ggacgaagtc
     1861 atccacggct tgagcgacga acccggcgat ccgggccaca gcacgatcga cagggaactg
     1921 gaagcggcgc gcgaggctta catcgcggag gataaggatt cgtcgctcca gcacctgacc
     1981 aatgcgctgg ttatcctgag ccacgaagcc gcctggtccg cgggggaata cgaggcgacc
     2041 ctccgcgagg ctatcgcgga actcgaaaaa ctggcccatg aggtccagga aggccagatc
     2101 gagcgcgacc agattggcga ccggtttaag gatgcctatt cgaaggtcta tgccgggctg
     2161 tcgcgctatc acctgagccg cgccgaggga atggtccggg agcgccagac gcgcgagggc
     2221 atcgtccgca acgaccagcg cgcgacggag accgaatcgg ccgcggtggc aacgcccgcc
     2281 ccgctggcga tcgacgtcga aacaaccccc gcccagacca ccgacgacga cgagcattgg
     2341 cgcgtggaac tgggccgcca gatctccggc gccaccgaaa acgtccgcgc ctggtcgcgc
     2401 gagaccggcc gggagatccg cgatgaaccc gtcatcgccg aactgcagga aatcgccggc
     2461 cgcatcatcg ccggcaccgg ctgggccacc gaggaagtcg gcgaagcctt gaaccgtttc
     2521 ggcgaaaaga tcaagcagta cggccgcgac cagaccgatc aggggcgcac gaaacagttg
     2581 agcgaccccg atgaagccgc cgacgaacgc gccgagatgg aacgcgaagc ggcgaaacgc
     2641 gcgcgcgacg ccgccgagga agcggccgag gaccattgac gccggcttcc gctgcgccgc
     2701 caaaagaaca cgccacccgc aacggcctgc ggccgcggca ggtggcgctt gttttcggcc
     2761 cccctccatt cggggagcgg catgccggct tattcggccg ggggcatcgc cggcggcatc
     2821 ggcgtcgtcc cgggcggcgg agcgaaaccg gggggcatcc ccggcatccc aggggcgccg
     2881 ggggcgggcg cttcgggtcg accaatcagg ttcttcgctt ttagctgcca gtagaaaata
     2941 aaactggccg cgaccagcag cacgacgatc accacgatct tgaccggctt gctcatgaaa
     3001 tctctcctcc tgacgtgggg tgacggttcc gcgcacctcg agctggggga ccacccgacg
     3061 ggccagcact acggaaccca ccgcgatggt gtgacaaata taccttgggc cggaaatacg
     3121 ctagctaatt ctgcgcgcga aagcgaaaag taacatgcga cccgccgacc ccggccccga
     3181 accctccgaa tcgcgattcg gatcgggatc gccatcgcca tcgcgatcgc cagcgccttc
     3241 gcaatccctc tcgcccgcca tccaaccgat caccccacgc ggagcccttg attcatgcag
     3301 cgccagccac ctccctctcc ggcgcgccct accccccggc ccctctcgcg ccggcgcaag
     3361 gcggccttcg ccctcgccgc cgccctcctc ctgctcctcg cgctcgaggc cggcgcccgc
     3421 ctctggctgg tctatgaccg ccatctgccc gcggcccgcc ccgacctcgc cgcgctgacc
     3481 tactaccccg agctgcgtcc tctcctgctc gccgacccgc cccccggcgc cgacacctgc
     3541 gacgtcctgc tgctgggcgg ctccgtgctg ttcgaatact acggcggccc cgccctccac
     3601 ctcgaggcgg cgctcgagca gcgcctgcat cgccccgttc gcgtctggaa tctcggccgc
     3661 ccgtcgcacg cctcgcgcga cagcctcatc aaataccgct ggctggattc gttgcggttc
     3721 gacgccgtcg tcgtctacca cgcgatcaac gagctccgca ccaaccatgc ccccgccgac
     3781 cgcttccgcc tcgattatga ccacgtcccc tggtatcgcg aggtcaacgc gctcgccggc
     3841 cacccggctg gcggcttcag ccgggcctgc gccctcccgc tcctggtccg ctacgccccg
     3901 ctctggctgg agcgccagct ccgtccgcgc ggaccgctcg cccccgacgc catgcccggc
     3961 cccgaggatc tgatccacgg cgacgacatc aaatcgacgg cgagcctcgc ggccaatctc
     4021 tccgaaatcg cggccatcgc ccggcgccgc ggcgacccct tgctcctcct gacctacgcc
     4081 tggtggctgc ccgacggcta ctccgatgag gacctgctgc acgatcgcga actggcgatc
     4141 gcccgggccg aggcccgccc gcccaccgtc gacggcccgc gccggctccc cttcgccgat
     4201 ccgctttacg ccgatttcgc ccagcccgtc tccatctggg gccggcggga acaactggtc
     4261 aagggcctct cgctccacaa cgaggtgatc cgcgccgtcg cgcgcgatca cgccatcccg
     4321 ctcgtcgacc tggcggcgcg gatgggcgac tccggcgact atttcattga tatctgccat
     4381 ctgagccccg ccggcgtcgc ccggctggtc gacggcatcc tggacggtct gaccttgctc
     4441 agcttccccg cggcccccgg cgatcccgcc cgccaaccag cacacagcac ctgacccccc
     4501 cgccggctcg gtgtggaaca acaaccgatg tggggctacc agccggtgtg ggaccggcgc
     4561 cctcgtcggt cattctttga cccgcgttcg ccgggatgcc tcgagtctgg ccgttcgcgc
     4621 atcccgccgg gaggccagaa tgcagccgag ggttgagcgc agcgacacca ccggtctccc
     4681 ggccccccac ccccatcctt ttttgattga cattcccacc aattgttcac atcttaatcg
     4741 tcgatatggc cgcaatagcg ggcggcccac gccgtccccg ccttccccca atcggcgact
     4801 tcgcttccgt tcacggggtt aaccggccac ccacaacgct ccgacctgag gaaaccctgc
     4861 ctggcggtcc ccaatccgcc ttcaccccac agatcccgtc gatacagcct ccacgacgtt
     4921 cgctgtctct tcccgcgggt gcgcaccgcg cgcacaatcg attcccgttc cacaaggagg
     4981 ttcccatgaa cccgtcgcgt ttgaggatcc atctgcttgc gcccgttgcg ctggcctgca
     5041 tgctggccgg gtgcggccca agcgtcgaaa aaagcatcgc cttccaggaa caggtctccc
     5101 gcaccgattc cgtcgaggag tcgctcaagg acctgagccg tcagctttcg atgctcgaca
     5161 ccgaattcca gcaggtctcg cgcatggtcg ccgagggcca ggggggcgcc ggcggcgccg
     5221 actccaacgc taccaaggcg ctcgaggccc gcgtcctgcg cctcgaacag gccttccagg
     5281 aatcccagaa gaccctgacc gcgatccaga acgccctcga ggagggcaag ggcgtgcgca
     5341 tcgcatcggc cgcccgggtg gcggcggccc cgccggccga gcccgcccag cagccccagg
     5401 aagtcaacga gcaggtcacc acggccaagc tgaaggtcgt cggcaagggc tcctcccgcg
     5461 cggtcgcggc gccggccccc gccccctcgc gcccggcggc tcagccccgg cccgccgtcc
     5521 gcgtcgaaca gcccgaaccg cgcgaggccg cgcgccgcag cggcatctat cacaaggtga
     5581 cccagggcca gtcgatcgat cagatcgcca gccactataa gacgagcgtc ggggcgatcc
     5641 gccaggccaa ccacttgccc gaacgggcgc ggttgatcgc cggccagcag attttcgtgc
     5701 cgacgggtcg ctagagggag cggatcgccg gcgccgtcag tcccgcggcc gtgtccgtcg
     5761 ggcgcggtcg cgggcggcgg cgatccgccg ggcctgtgcg cgcgcccatt ggcgcgcctc
     5821 cccggccagt tgccccagcg ccgccgcctg cccgcgcacg tcgcccggct cggggccgcc
     5881 caggctgccc cgccgcgcca gcgcctgttc cggccggccg gccgcatcca gttcctccgg
     5941 cgtcaacggc gggtcaatcc ccgcgcgccg cagtatctcg ttcagcgtcg ccgcgctgat
     6001 ccagccccgg gcttcatcgc gggcgaccgc ttcggccacc acatgatacg cttggcgaaa
     6061 cggcagatgg cgcgtgcgag ccagatgatc ggccaggtcg gcggccaggg cgaacccggc
     6121 cgtcgccagc tcgcgcagca ccgggcggtc cgcccgcagc ccgcccagca cctcggcgaa
     6181 cacccgcggc gcctccccca cttcctccac cagatccatc acccagtact tcgtccactg
     6241 cgtgtcgcgg ttgtagccgg tcagcagtcc gcgcccgacg ccggccaggt tggccgccag
     6301 cccctgcacg gccagcgcgc gcgcccgcgt cacttcggcg aaatccggat tgcgcttgtt
     6361 gggcatgatc gagctgccgc tcacatgcgc gtcggccagc cgcagcccga cccgcggcgg
     6421 cgtcgacagg aaaatcagat cctgccccag gctggccaga tgcgacatca ggatcgccag
     6481 cacccccccc aaccgggcct ccgcttccca gcgcgtcgag atcgcgtcca ggctgttggc
     6541 ctgcggcgcg tcgaacgcca gcagccgcgc gctcagccgc cggtcgatcg gccacgacgt
     6601 gccgaaactg gccgccgccc ccagcggact gacattgatc agcggccaca gcgcccgcag
     6661 cgccgccagg tcgcgcgtca gcgcgaagcc atgcgccgcc gcccagtgcc ccaacgtcgt
     6721 cggcatcgcc ggctggccgt gggtccagcc gggggcgatc gtctcggcat gcgcccgcgc
     6781 cagccggccc agcgtctgga cgagttccgc cgtcgccagc gtctgttcca gcagccggtc
     6841 gcgcagccac agccgcacgt cggtggccga ttggtcattg cggctgcgcg ccgtgtgcat
     6901 caccccggcc gccgcctcgt cgcgccgcgc caccagccgc tccagattca tatgcacgtc
     6961 ctcgagcgcc gggtccagcc gcaggcgccc cgcgtgccac gcctcctcga tccgttcgag
     7021 ctcctccaga atcagccggc aggtgcccgc gtcgatgatc cgccgccgct ggagcatcag
     7081 gcagtgcgcg cgattcgtcc acagatcgta cggaatcaac tgctcgtcgg cggcggggcg
     7141 ccccttcacg tcgcgccccg aacaataggc caggacggcg gccgacgggc cgccgctcgt
     7201 gcgcttggac cagatcgggc cggacgcggg cgggctggac atggaacgga ctccctcggg
     7261 gatgctggcg ccggctccca tcggcggctg cacggccaat gcgaacgact ccccatcatt
     7321 tccgttgccg gagccctccg caagcctttc gttcggcctc gaccgcactc gtggcaggag
     7381 gacggggccg cttcgaccgc tcccccaaaa aaaacggcgg attcagcgaa aactcagtgg
     7441 ctttcatccc cggccggcgc tatcctcacc ccttttctcc ggctcgccgg gtctcaactg
     7501 agggggtcgc caagcgatga aaaaacttga gtttcccgac ccatgacgct atttgaaagg
     7561 gacccttttg ggaactgccg tcacttcgag agttggttgg atctccgcgg gcgagccgca
     7621 gtgaacgata agaggggtgt aagttgagcc ggaacaggtg caagtgggcg atgggcgcgg
     7681 cgctggtgat cgcctgcgtg ctggccggtg gttgcgccac cggcggtgag agcgaacccc
     7741 agccggagga cctgaccctg ctggtcacgc tcaataagag cgtctaccgc cccggcgaaa
     7801 tcgtggaggc gcgggtgaag ctggccaaca ataccaacga gcaactcgaa ctgcccaagc
     7861 tgagcgcggc cgacggcggc tcgatttcct tctggtactc gctggccacc gagcccgacg
     7921 cttcgatgca gcgccccccc gtcgtctccc ggctcgaacg ccccgagggc ctgatcacac
     7981 tcgcgccggg gcagagtgag gagcgcacct tcgtcttgac ccgcctggcc gaatactccg
     8041 ggcccatgct gctccaggtc cactacgatc ccaacccggc cggctcgggc cgcaccgaac
     8101 cgaagatctt cagcaacccg gtggcctacg acgtcaaggg cgagctgctc tttgggcgcg
     8161 atccctccgg ctacttgatg gaggaggagg cgatcggcct ggccaacgcc gtcgcccccg
     8221 gcgaggtccg cagtggcgac gccatcctca ttgaggacga aatgggcttc tataagtggt
     8281 gggtcaacct gcaggtccag cgcgagggcg gccagaccca gaccctggcc tggctcgtcg
     8341 atccctactt cggccgggtg atcggccagg cgcagccctt ccccgagaac atgcagaacg
     8401 atccccgcac ccgcgggcag aaaagcttgc gtcgcgatcc gagccggccc gatcgcgtct
     8461 acatggaaga ttgagctgct tgcgccgtgg ccggcgggcc gcgcgccgcg cagtttgcca
     8521 aacctgggga aaggaggcca gggtatcgcc gattaagtgt taagcgctga ttgctagggc
     8581 agtcgcctcg gccgtcgggc cggggttgat ccgctgcacc gcccgcgtcg tccgtcccgg
     8641 atctccttcc gatccgcctc ggacttcccg gcatggccgc tgtccacgcc gccttcaggg
     8701 ggcgattcac ggttcagcag actgcccctt tggccctgca agccaccagc agcactcata
     8761 ttccaatgga ggagcgtcac atgtacaaga gactggcctg gctgtgggcg gccggttggc
     8821 tggtcgccac cagtctggcg accgccgcca cggtggacga aacccgtcac ctgcccccca
     8881 acgactaccc gtcccatacg gtcaaaatcc tgcggaccac gaataaggcg cagacccaaa
     8941 gctacgtccc cgtggtcttc acgatgcgca acaacaaccc gtttaacgtc atccagtacc
     9001 tgcatcggcc gattcagcac gaagagggcc tcctcttcac cttcgtctcg cccgagggca
     9061 acggcggaca cgtgctctat tgcgtccccg aatacatgat cgagtcgctc ggtcggctgg
     9121 ttgccgccct cgatcgcccc ggcctgacca cctcggccgg aaccacccgc atctaccgcc
     9181 agctcaagca ccggcgcgcc agcgccaccg acccgggatt catcgagacc gctgcttcgt
     9241 tctccaccgg caacggcaat gacttcctga tcgacccctg ggccaacgcc ctctactacg
     9301 aggacgccga ctccggcgcc atggccctcg ataacgccct gaccgactgg ctcgacgtcc
     9361 cgaccgccat ggtcgacgtc atggtcaagg tctacgaact ggccgcctac aacgacgcga
     9421 cgatcggcct ggattacatc tcctggaaga atggccccgg ccgcgacctc ttcgccgtcg
     9481 gcctcttttc cgaatacgcc agcgtcgaac gcgccggcag caacctgagc gtcttcccgc
     9541 ccaacgccga tccctcggcc ctcggcctgc ggcaggggct cagcacgcgc aacaacctga
     9601 gcgccagcgg ctggaattac gcctggagct acaacgtcga ttcctccttc ttcgattacc
     9661 tccagaccaa gggcaaggcc cgcgtcctga accgcgcccg cctctcgatg ctgaactcct
     9721 ggccggccta cctcgattcg ggcgatcagg tcatgtccta cgccgtcggc acctccaatc
     9781 cctccggcat ccgcgactcg ggcgagatct tccaggccaa caccggccgc ctgatgaccg
     9841 gcacgaccaa ccagatcgtc accagagaac ccgaggatct ggagcagctg atcctgaacc
     9901 tcgtcctcaa cgggttcgac agcggcctct ccggcctctc gccgtattcc tattgcctcg
     9961 aaccgatcga gaccggcttc ttcctggcgc tcgacaatgt catcggcgaa cagtcggtca
    10021 cgttggaagt cgacatggag tggagcgatt acaccgcgtt tgacgacacc ggcttcccgc
    10081 agatccagtg ccgcaagctc cacatcgagg atctgcgcct cgccctcggc gacgaaatca
    10141 tcatcggcgg cctcaagcgc caggcccacg ccgtcgcgac ccagaaggtc cccttcttcg
    10201 gttcgctccc gatcgtcggc tacctcttcg gcggcgagga caacctcaac aagcagaccg
    10261 aactggtcgt cgcgatcaag cccgtcgaaa tcacctccta ccagatcgcc cgggatgatc
    10321 aggcggtcat cgacaaggcg accggccagg ccgaaatcgt cgagccggat accacgatgg
    10381 gcttcgatca atacatcttc gacaaggccc gcgatgtcga ggccatgccg ttcaactgag
    10441 cggcggcccc gtgccattcg ccttccaacc ctgccactga atgagaagaa ggagtcagct
    10501 catgacgacc aaatggctta cccgaagcat gagggtcctg ggcgtgttcg gactgtcggc
    10561 gggcctgact tgggccgcct accccgagtt cgaaaatgtc cagcggctta acgccgatcc
    10621 ctccccgacc aagtcggaaa gcaacatcaa cgaatacacc taccaggacg agcagatcaa
    10681 catcgtcccc tccgacggcg tggtcaaggt cctgcgcacc gatcagaaga tcctgatcaa
    10741 cgactacgtc accgccgtca tcccgatcaa acacgcggag ccgcgcgagc tgcgcaacgt
    10801 cttccgccag gtcaccgccc tcgaaggcgg tcgcgccgag gtcatccgcg acaaggaaac
    10861 cggcgagaac ttcctccagg tcatcgcccc cgaatacatg atcccctatc tgcgcgacgc
    10921 cgtcgccgcc ctcgatgatt cgtgggtcga ggaatacaac gacggctcct cggatctcta
    10981 cctcaacatg cagcatcgcg atgccgccga ggtcgatttc ttcgccgcca actacgccgg
    11041 caacgacggc ttctccacca tcgacaccac caacaacagc gtgcgccgct acgacgagat
    11101 ctatcgcaac gagaaatacg ccgccgccgc cacgctcttc gacatcccct gcaaccaggt
    11161 cgagctcgaa atcaaggttt acgaggtcaa cacctccaat gacctgaaac tcggcctcga
    11221 ctacatcaac tggcagaacg gccccggccg caacctctgg tacttcgtcc agtccggcta
    11281 cagcgccgag cagcgcgcct tcggccgcac cagcgtcttc gatccgttcc tcgacgcccg
    11341 cgccccgatc catcccggcg tcaccgacaa gacgctcgtc caggataccg ccgctcgcgc
    11401 cggnnnnnnn nnntcaacta cctgttgacc tccaatttca tcgacttcct ccaggtcaag
    11461 ggcaaggccc gcgcgatcga tcaccagacc atcacccttt gcagtgggtc gtccggttcg
    11521 atcggcaccc tggaccaggt cctggcgttc gtcgacaaca tgaacgacat caccgacttc
    11581 gcggatgaga acccgaccta catccgccgg cagaacaacg gcatcggcgt ccccgattcc
    11641 acccccaatc tgctcgccaa gaccggtttc ggcgatgggt tggaccagtt cttcctcgac
    11701 gtcaacggca acggcatccg cgacttcatc gacgagaaca acaacggcgt ccgcgacttt
    11761 atcgacctga acaacgacgg catccgccag gaaaacgagc cgttcaccga gccgctcgag
    11821 gacctgctgg gcgactcgcc ctcctccgtc cgcgtccagg attcgacccg ccggctgcac
    11881 tatgaaaacg ccggcaccgt cggcatgaaa gtctgcgtca ccccctacgt cggcctcgag
    11941 tccatggagc tcgatatcga cctcgatatc ggcgagatga acggcatcgc ccccaacggc
    12001 acgccgatca tcaacttccg ctcgctcgcg accaccgtcc gcctgatgga cggccagccc
    12061 ttcgttctgg ccgcgcttaa gcaggtcaac gacgtcaaga gcaccgccaa ggccccgttc
    12121 ctcggcagca tccccgtcgt cggctacctc ttcggcggcg agaccgacgt gaagcgcgag
    12181 aacgacgtcg tcatcacgat cacgccgcac ttccagcttt ccgatcaggt caagctctac
    12241 aagccgccca gaatcgacac gctgcagatg atcatcgacg gcaccctgcc ccagggcgcc
    12301 ccggcgatcg actacggcta cgatcagtgg ctgcttgact gcggccacaa ggatcacgcc
    12361 gcggccaacg ccggcgccga tgaggacatg agctgaatct cggcccgcgg ggcccccggc
    12421 tccgcaccgt ctgattcaaa cccaaccggc agccgccttc accggcgact gccggttgct
    12481 tttttttacg gcatgcggct tgctctgctt cgattccgat gacgcccacc catcggagga
    12541 ggcaaaccat gggcagccca cgcgaagaaa aaccccgccg gaccccggcg cgccgcatcg
    12601 acggcgacca ccgccggacg ggccgcgccg acgaaccctc gaaccccgcc cgcagcggca
    12661 acaaccggac ggccagccac gtcgagaatc ccgtccgcga cgaggaggac ccccgcatca
    12721 agagcaagcc gggccagccc ctccacagca aaaaggaaga tatggatcac gtgtaatgcc
    12781 cccgcgccgc cgcgcgcaac gccctcagcc cagcccgatc aggccgcgcc gcgccagcag
    12841 cagcgcgaac cgcaatgtcg cctcgcggct cgcctcgcgt tcaaacccga accgctcgcg
    12901 catccgctcc tcgatcgtct ccagcgtatc gattccgttg atcaagcccc agaaaaacgc
    12961 cccccgcgca tccagctcga cctggataaa aaccgaccgg cccagcttgc gatcgagcca
    13021 gccgcgcacg cctccgcgcg ccggccgccg gcggcggagc tgcacgcgct cccccccctc
    13081 cgaccgcgcc tccaccccgt cctgccggcg cggcacgagc cgccgccacc cgtcgcccgt
    13141 ctgcgcaacc gccgtggcgg cctctccccc ttcctcagct ttgctcgaat tcccagccga
    13201 attggacgta ctcatggctg aagcttccgt cctcatacag atccaccaca ccgtaccccg
    13261 gccaacactc ctgatacggc ccgtcccacc accccgcgca caccgccccg tcgcagatat
    13321 acgtcacgcc gcaatactcc acccggtcga ccaggtgcag atggccgctc aggcagagcc
    13381 gcacgttggg atgtttgagg aacagatcct tgattcgccg cgcgtcgatg tgcatccacg
    13441 cgcgcggcac cacccagtcg cccggcttct cattctcccc gtcgaaaaac gacgccgcgc
    13501 tcagaatcgg tatgtgactc agcaccacca ccggccgcgc cccggccagc cgcgccagat
    13561 cttcctccag ccacgcgaac tgctcgtcgt ccagccgcgc gaaataataa ttgcccccgt
    13621 ccgagctcgt gctgtccagc acgatgaaat gccacccatc gcggtcgaaa ctacggtaat
    13681 gccgctccac gccgaactct tccagcgccc agcgcttgcc gaaccgctcc tcgtcccccg
    13741 tggttcgcgc gctcgccttg tcccaccccc agatgtcgtg gttccccagg cagtggatca
    13801 ccggcagatg gcactcggcg gccagcaccc gctgccacac ctcccactgc gtccgcgcgc
    13861 gctcggcatc cgtcgtgaag cagtccagaa tgcagtcgcc gccgttcagg atcagttgcg
    13921 gcgggtcggc caacccctgc gcgtgccgca gacacgcggc taagccttcc ggcgcccgct
    13981 tctcgggttg aacgtgcacg tcggtcaggt gcgccagccg cagcgaccgg cgcgcgcccg
    14041 tcccgcccga ctgccccttc gccgaggacc acgtcccctg gcagccgatc aatgtcgctg
    14101 cggcggtggt caggcccgcc gaggccagca gacggcggcg tgtcatccga ttcggcatgc
    14161 tccatctatc tccccgggaa tttcagggca acgcctgatc cgccgcgggt acgatcggcg
    14221 cggcgtcgcg ccctgcccct tgagtctacc cccctcccgt cgccaccgtc aaaccccgcc
    14281 cctgccggca tccaccccca acggcccgcc caaacattcg gctcccggct ctcgtccctc
    14341 ctcctcggct ctcgaaaaca cccatctccc ttcatccccc cggccccatc cctcgccccc
    14401 ctactcgacc gtgtaaagat cgcctgtccc ccgccctcaa ccccgccttt ccttgtccgt
    14461 ccgtcagccc cctcaccctc cccatttgac tggatttgcc cctacgtccg cgtccccccc
    14521 ccccccccc
//
LOCUS       DAOPCO010000075        14317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4272_length_14317_cov_4.987940, whole genome shotgun sequence.
ACCESSION   DAOPCO010000075 DAOPCO010000000
VERSION     DAOPCO010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14317
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4272_length_14317_cov_4.987940"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="PLS90_07230"
     CDS             complement(<1..1992)
                     /locus_tag="PLS90_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02236.1"
                     /translation="MPPSSRFSLPFGLVLALACVLPGGAFALEGFPEPTDAAAPRPEI
                     FRYSLDNPPIETLLVDLDGDGYRDFLTLENGRLNLRNDTGFPFWRDRNPDVDAAAFTR
                     IVTAANLDHQGYGEIVAETTEDVWIIDGYSMAARARVAQARVLGLTDLNEDGIAEIYI
                     ASREADDPAFPPLSVFSLTADGWTELWSSPGCRPESKDGQDAYQIHSLPDQLHQFDLD
                     EDGQPEILLRLPAGGGWIGVGFDGQGRFDEKWEFTAIQDGNSRFTFLDWTDGNRDTYA
                     DIIGYLVDKRRLMNMDIVSLGDPLMTIPLRQLEEPAVSAAGLRRDRVKAGEWPLAQRD
                     HRLSGRSPVKMAIAGAPRESWSYSLGHTPGGLQALELDGRPGADLLVVARNRLTAYSA
                     DGDRLWASDQLGGVVLKGVYDLNADGRNELFIQFRPVLAQPTLALLDAATGEILWKLT
                     TQNYVGSFPRVRIGHFLPDRPGAQLLATGTWGDQEVFFFDFSDGFQAAAQPRWHRILH
                     HVIYPTEDFYDYDGDGAFEFVQVEHSRLRIYDLATGETERDLEWDRHRSYGQMLPLNI
                     DADPYFELLITGDGSVQQVEMIDLDTTATTLRWKVPGKLGHRRYGPGDLIRLKFEPQR
                     VGDVDGDGELEVVYLKYNETGDQKWHFYQRAATDGRIERD"
     gene            2515..3129
                     /locus_tag="PLS90_07235"
     CDS             2515..3129
                     /locus_tag="PLS90_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02237.1"
                     /translation="MNARLKAFTLIELLIVVAIIAILAAIAVPNFLEAQSRAKVSRSR
                     SDMRSIATALEAYRIDNNSALPASAMSAWWCERWGYWERVTSPVAYMTSVPEDQYYKS
                     LRTWGDDYFYFFPVYDKNIMNALLHGGTAPGMWSRVLTPVGGEARGWLTTYIEPAFSL
                     WGRAGSNYMIRGFGPISVGADQVYIEPYDPTNGTISRGNLYYYN"
     gene            3191..5353
                     /locus_tag="PLS90_07240"
     CDS             3191..5353
                     /locus_tag="PLS90_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK02238.1"
                     /translation="MNQRILMLGVVVACIAWGATSARAVNLEDDPGLTITASVGDLGP
                     ANGVEKLIDDDPATEWISETSVGTGGSATLTFTWDHPVPIKRVEVTHNFYPPDQYRIE
                     TWNGSAWVEHVQFQYGSAQSTDLAQNGAMPVDFPAQTDRLRIVIDFAHSANYPRLAEV
                     TVSDTLADVPDNLEDVFGLTVVSGSGNLGGYPVTNLIDNDTNTEWVASLSTGIGGPDT
                     VTFTWPSPVHIEYVLINMGYYNLFEYTVETWNGSSWVVNDGVVDDTTTPYIVKVHKNG
                     AMALDFPDQTTQLRINIQAANQDNYARFAEITILSAAPAEPSRYEDYDLSTVNTFQFH
                     RLNQQTPFVDLNQDGLIDLVAPNGAGARLFFQQANPTEQFSADTNDPTMTALLGNNMD
                     NNVTGFYIGDFSNDGIADVAYLGAYSNNMLPANVKFHNGLGSGEPLPEPTSEFELGNG
                     VIFVYYTAVGDVNGDGRLDLAFRPNPNHAMKVLIATQQGNGTFAITSKLNAGTEEGAV
                     LLYDVDDDGKDDLLTVNGNNRVDLFLTTHIPWVNDDAGTTKTASYTVSGLGAGAVDLD
                     VGDVNGDGGLDLVVSGGTKLSVWTDQSKLWQPSASSIQPDLSFDAGESVVGLEVIDLD
                     VDGRDDILVGTTTGRVLLFLARNADPLFENTPDQIYGVNAFSNVGDVIVQDLDGDLAY
                     DLIAYEDVTGAMTVWMDLEESGSVPQPPTHARHWAIVE"
     gene            5517..6659
                     /locus_tag="PLS90_07245"
     CDS             5517..6659
                     /locus_tag="PLS90_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02239.1"
                     /translation="MDTVAVNHRRCYLETLLYGNPERIPLTPGEPRESTLRRWHAEGL
                     PEGMHHLDAAAERLGIDLQRPATPPVDLGVDFRMIPWFEEKVLEHKDGHYLVQDWMGA
                     ITEISDEYDYTYIRSAKDFVTRKWHRFPVQTREDWHEMRRRYDPDTPQRLPADFAARC
                     ARLRKRDDYAGVSFNGPYWQLREWLGFENLSVLFLTQPDWVEEMIEFWSGFVSRMLER
                     ILSRVQLDYIWFSEDMAFKAHPMIGPDMARRFLLPTWRRWADLARRYGVQIVDVDSDG
                     YIEDLIPVWIEAGVNCCDPIEVAAHNDILRFRERFGTAMAYKGGIDKRAIAAGGRAIL
                     AEIKRNEPVIRGGGFIPGCDHGVPPDISWDNFVEYTRLLAQVTGWL"
     gene            6750..8222
                     /locus_tag="PLS90_07250"
     CDS             6750..8222
                     /locus_tag="PLS90_07250"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02240.1"
                     /translation="MTDIKIAVIGGGSVNWMPVLMRDLYQLEEATGGEIRLCDPNQEH
                     TRAVVALLQRYNELRGKRYAISVHEDRRRALAGADFVLTTFSPGAMDAFHNDLEIPIK
                     YGVRQPVSMTVGPSGISAAIRTVPVAYAIVEEMEEVCPRAWLLNVTNPMTTVTRAMNM
                     ASRGVPVIGMCHEFHEFSRYVGVILGLERPAGIGVMDYLYRWLAEQGLEYRVAGINHF
                     IWLTEARLHGEDVIPRIRQFARDHWDLRPAAGNGEPPDAWANYSAAKLALCRTFGYMP
                     LAGDRHLIEFWPSLCNQANGFGMRYGVHKTTVDLRRHMKERQLAEIERVARGEQAVHW
                     QTSGEEMSAIIRSVLTGRPTTAIINAPNRGQIDNLPDEVVVETLATVSSAAIEPMRCG
                     PLPAAIAALCRLHCAVQELTVRAALAGDRDLLIEAFSLDPLCAAVDFAQIPRLVDDLL
                     EANRPWLPRFFETRARRGGNGRATPVAHAEELSAAVPGGH"
     gene            8224..9282
                     /locus_tag="PLS90_07255"
     CDS             8224..9282
                     /locus_tag="PLS90_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK02241.1"
                     /translation="MEGSIHRVRQVLQGRRPDRAPLFDLLRNDAVLSHFGGRRLDFDN
                     AQEVVYRAHGAALDATRPAVRLPQPERCETLPDGRTMKYLRWTAWQAPRFYASPEEFA
                     QERARYVETFDAAWNAERQRALDEELAFFARDRHRLGDLFYFPATRGVGIQPLYTAIG
                     LEQFCLFMLYYPQLIDDLLECSALEAIAWCEHLPADHGIEAVFVSEDMASKNGPLFSL
                     DWLDRHYFPRLQRVIAAYHQRGILVMFHSDGNLMPVLDRLVAAGIDGLNPLEVQAGMD
                     PVQIHNRYPKLFLSGGIDVSNLLPFATPDEIRRVVRRTLEGTGGRLLVGSSTELNQEV
                     PLENYLALHETALNFTLD"
     gene            9355..10149
                     /locus_tag="PLS90_07260"
     CDS             9355..10149
                     /locus_tag="PLS90_07260"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK02242.1"
                     /translation="MTSQTQTTEPMRLTEEQLEAYRRDGYIMIENLVTPEELKQLHHR
                     QREYTHCGRDASKLSIQVEPRIQRGEVKVDHPGDGIRKIDGLIEHDDLFRRLGTHPNI
                     LEILEQILGPDIKLYRNTLLLKPPLVGSAKGMHQDSPYWPIEPMELCSCWFTLDAATP
                     ENGCMGVIPGAHRRGPLPHHQVPDDFVMDEASYDMRDVKLVPMPAGAGLFFHSLLPHV
                     TAPNTSRHWRRAITMTYMSARSRYTGEGESPNFMTVRGRGYPGCVG"
     gene            10468..12102
                     /locus_tag="PLS90_07265"
     CDS             10468..12102
                     /locus_tag="PLS90_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK02243.1"
                     /translation="MMSTRRSTTRFLRLRIAAQWLALCATGALLLCRLGQPPRSDPGR
                     DSVDADGTQVVDLWCGVIMGRWDESALQEAIDRFNARHPDIRIEPSLIPYTVYNAKLN
                     LAIACGAPPDIVYNAVNFVAGMPRHTELANLLVPVPDSMVTPEMRRGWGETILKTITQ
                     DGRIMKFPDRAYLGGMMIQGNLDYLEMAGVDVKHYLSEGWDYETFKRELKRVQQVVRE
                     RTGREVYAFGLNLVNVPTFLFRFLLPPIIGADATERSLLAYDPAAGRYVLDPAITPEI
                     LAQPLQLMQDMIHRDQMWGRKYLGMDFGQIGIEIYDRLELVTTFQDTPGNSIYSQLLR
                     NRDFAQGLREAPLRVTNLPVPTPTGDEPFLFSVGTDGWGVMRQVPYKGDAHTSAALQV
                     ARYLASPEVQALRFRYSDGMRNTLWPDPDAVEPFIRDLEDPIRQDPWLAYLWDLYESW
                     MKEAETYLAEEEQFPDIHDRRALLNLLGVASYGGYFYSGQGKQTLERVLFNQVEPLQA
                     AREMLAGAARLIDDYYRQRSSGDPQDADRRQISSVR"
     gene            12307..>14317
                     /locus_tag="PLS90_07270"
     CDS             12307..>14317
                     /locus_tag="PLS90_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02244.1"
                     /translation="MELKTDELVVSLDETQGALAIARRDGRGFALRDAAPRVDGDFGV
                     VRAAYRPRIRRESFADPLGQGERLIVDFADLPGGSEIQWRVQLYRERPWVSLTADLRR
                     PNGLLTRLSPLAGSIVVPGGNRAEQVRAFVHSGSQMPCGSCDLGAGGIATAHVTDIDC
                     DLQAESDDRRFKSEFLTALSEPTTGLGMALGFYSLRRAWCSIRMQPADDGIIHLLAAG
                     EYYHHNLAADARRMLGESLLVDLTLDPLTGLENYARAVEAQVQPRFNRELGRGFHNPW
                     FYLGNQTRGEHMLHDAARLAECILSKYYGISVVMTGLWQKQHPVEGPGASQYEDSLNS
                     VGMGEELVDENRYPDGGLERLEREMRATGTEVACGGFFFQAGSRSTIRRRHPDRVING
                     RIDYTHPACREWLRGIIEPFVRRGARYLWADFNDNAIYGVQHDPTMVKGFENTRAGMQ
                     VLRDALGPDGLIGTYTSPTICYTGIVDRMRMGLDMVPFNHWSSLREIVRNMAAAYFFH
                     QRMWMNDPDPIFVCGESDDPATLEEARIRLALGTICGSVCATGERFYNFTPQRMRLLT
                     TALPSAGIAARPLDLMRRDVPEEFLTVLRRDWDAWRVVSYLNLNEAERTYPMQPDALE
                     LDSAEGQLVFDFWTQRCLGALTPGQTVTVPARTCRVLLIRDKKPHP"
BASE COUNT         2544 a         4749 c         4600 g         2424 t
ORIGIN      
        1 gtcgcgttcg atccggccgt cggtcgccgc gcgctgatag aaatgccatt tctggtcgcc
       61 ggtctcgttg tatttcaagt agaccacctc cagctcgccg tcgccgtcga cgtcgccgac
      121 gcgctgcggc tcaaacttca ggcggatcag gtcgccgggg ccgtagcggc gatggcccag
      181 cttgcccggc accttccagc gcagcgtggt ggcggtcgtg tccaggtcga tcatctcaac
      241 ctgctgcacc gagccgtcgc cggtgatcag cagttcaaaa tacgggtccg catcgatgtt
      301 cagcgggagc atctgcccat agctgcggtg gcggtcccat tccaggtcgc gttcggtttc
      361 gcccgttgcc aggtcgtaga tccgcagccg cgaatgctcc acctgaacga attcgaacgc
      421 gccgtcgccg tcgtagtcgt agaaatcctc ggtgggatag atcacgtggt gcaggatgcg
      481 atgccagcgg ggttgggcgg cggcctggaa tccgtcgctg aaatcgaaga aaaagacttc
      541 ctgatcgccc caggtgccgg tcgccagcag ttgggcgccg ggccggtcgg gcaggaagtg
      601 gccgatgcgg acgcgcggga agctgccgac gtagttctgg gtggtcagct tccagaggat
      661 ctccccggtc gccgcgtcga gcagcgcgag cgtcggctgg gccagcacgg gtcgaaactg
      721 aatgaacagc tcgttgcggc cgtcggcgtt gagatcataa acccccttga gaacgacgcc
      781 gccaagctgg tcgctggccc agagccggtc gccgtcggcg ctgtaggccg tcaaccgatt
      841 gcgcgccacc accagcagat cggcgcccgg ccggccgtcc agctccagcg cttgcaatcc
      901 gcccggcgta tggccgagcg agtaactcca gctctcgcgc ggagcgccgg cgatcgccat
      961 cttgaccggc gaccgcccgc tcaatcgatg atcgcgctgc gccagcggcc attcccccgc
     1021 cttcacccga tcccggcgca gcccggcggc ggagaccgcc ggctcctcga gctgacgcaa
     1081 cgggatcgtc atcagcggat cgcccagact gacgatgtcc atgttcatca gccggcgctt
     1141 gtcgaccagg tagccgatga tgtcggcgta cgtgtcgcga ttgccgtcag tccagtccag
     1201 gaacgtgaag cggctgttgc cgtcctggat ggcggtgaac tcccattttt cgtcgaaacg
     1261 cccctggccg tcgaagccga cgccgatcca tcccccgccg gccggcagcc gcagcaggat
     1321 ctcgggctgg ccgtcctcat ccagatcaaa ttgatgcagt tgatccggga gcgaatggat
     1381 ctggtaggcg tcctggccgt ccttgctctc cgggcggcag ccgggcgagc tccacagctc
     1441 ggtccagcca tcggccgtga ggctgaagac cgacagcggg ggaaaggccg gatcgtcggc
     1501 ctcgcgcgag gcaatataga tttccgcgat gccgtcctca ttcagatcgg tgaggcccag
     1561 cacgcgggct tgggcgacgc gcgcgcgcgc ggccatcgag taaccgtcga tgatccacac
     1621 atcctcggtg gtctcggcga cgatttcccc atacccctga tggtccaggt tggcggcggt
     1681 gacgatccgg gtgaacgcgg cggcatcgac gtcgggattc cggtcgcgcc agaaggggaa
     1741 gccggtgtcg ttgcgcagat tgagccggcc attctcgagc gtcaggaaat cgcggtagcc
     1801 gtcgccgtcc agatcgacga ggagcgtctc gattggcggg ttgtccagac tgtagcggaa
     1861 gatctcgggg cggggggcgg ccgcgtcggt cggttcgggg aatccttcca aggcgaacgc
     1921 ccctcccggc agaacgcagg cgagggccag gaccaggccg aatggaaggc tgaagcggga
     1981 ggaggggggc attgcgcaat cctttggacg ggggatcggg ggaccgaacg gcgggatgac
     2041 cgccgggagc cgcatttttt ccgtgcaaac gatttgacaa tatactcccc cctccctata
     2101 ctacaaccca gaaatcgcct catcgtctcg acgccgggct gtttgccgcg caacgatccg
     2161 cgactccctc aggcctgaca agcgtccagt cggaccgggc cgccaaccaa tcgctcagca
     2221 ttccgtgcga ggaatccaca ccgtcagcgc gcgtgcgcaa aaacggggaa ggttgggggc
     2281 gggaagcatg cgggggggtc actgaaggcg aacaggtaag cgcttacatc ggcctgccgc
     2341 gccccgcgtc cgatggtcaa ccggaaggag gtgcattcgc tccagcacga tccattcact
     2401 ttttttcaca tgtcaacatc gtccctgtaa ttggatggcg gttcagtccc ggcccgactt
     2461 cggccgcagc cgatcgtcaa cgacgcccca cgggggcctg atggaggaag atccatgaac
     2521 gcaaggttga aggctttcac cttgattgag ctgttaatcg ttgtggccat catcgccatc
     2581 cttgcggcga ttgcggtgcc caacttcctg gaagcgcagt cacgcgccaa ggtcagccgc
     2641 tcacgctccg acatgcgctc gatcgcgacg gcgctggagg cctaccggat cgacaacaac
     2701 agcgccctgc cggcctcggc gatgtcggcc tggtggtgcg aacgctgggg ttattgggaa
     2761 cgggtcacgt cgccggtggc gtatatgacc tcggtgccgg aagaccagta ttacaagtcg
     2821 ctgcggacgt ggggagatga ttacttctac ttcttcccgg tctacgacaa gaacatcatg
     2881 aatgccctgc tccatggcgg cacggcgccc ggcatgtggt cgcgcgtact gacgccggtc
     2941 ggcggtgaag cccgcggctg gctcaccacc tacatcgaac ccgccttcag cctctggggg
     3001 cgggccggct ccaactacat gatccgcggc tttggcccga tcagcgtcgg cgcggatcag
     3061 gtgtatatcg agccctacga tccgaccaac ggcacgatca gccgggggaa cctctactac
     3121 tacaactgag gcgtggacgt ccggagcggt caggctcccg accgaacatt catcatgaaa
     3181 ggcaagaacc atgaatcagc ggatcttgat gcttggcgtc gtcgtggcat gcatcgcctg
     3241 gggggcgaca tcggctcgcg ccgtcaatct ggaggatgat ccggggctga ccatcaccgc
     3301 ttcggtgggc gacctcggtc cggccaatgg cgtcgaaaaa ctgatcgacg atgacccggc
     3361 caccgaatgg atcagcgaga cctccgtcgg aaccggcggc agcgccacgc tgacgttcac
     3421 ctgggaccat cccgtgccga tcaagcgcgt tgaggtgacg cacaacttct acccgcccga
     3481 ccagtaccgg atcgaaacct ggaacggcag cgcgtgggtc gagcacgtcc agtttcaata
     3541 cggctcggcg caatcgaccg acctggccca gaatggcgcc atgccggtcg attttccggc
     3601 ccagaccgat cgcttgcgga tcgtgattga tttcgcccat tcggccaact atccccggct
     3661 cgccgaggtg accgtcagcg acacgctggc cgacgtcccc gataacctcg aggatgtctt
     3721 cggcctgacc gtcgtcagcg gctcgggcaa tctcggcggc tatcccgtca cgaacctgat
     3781 cgacaacgac accaacaccg agtgggtggc cagcctgagc acgggcatcg gcggccccga
     3841 caccgtcacc ttcacctggc ccagcccggt ccacatcgaa tacgtcctga tcaacatggg
     3901 ctactataac ctcttcgagt atacggtgga gacgtggaac ggctcgagct gggtcgtcaa
     3961 tgatggcgtg gtcgacgata ccaccacccc gtatatcgtg aaggtccata agaatggcgc
     4021 gatggcgctc gacttccccg accagaccac gcagttgcgc atcaatatcc aggccgccaa
     4081 ccaggacaac tacgcgcgtt ttgcggaaat cacgatcctg tcggccgcgc cggccgagcc
     4141 gagccgttac gaggattacg atctatcgac ggtcaacacc ttccagttcc atcggctcaa
     4201 ccagcagacc ccgttcgtcg acctcaatca ggacgggctg atcgacctgg tcgcgcccaa
     4261 cggcgccggc gcgcgcctgt tcttccagca ggccaacccg accgagcagt tcagcgccga
     4321 tacgaacgac ccgaccatga ccgcgctgct gggcaacaac atggacaaca acgtcaccgg
     4381 gttctacatc ggcgacttca gcaacgatgg gattgccgac gtggcctacc tgggggccta
     4441 cagcaacaac atgctgccgg ccaacgtcaa gttccataac ggcctcggct cgggtgaacc
     4501 tctccccgaa cccacctcgg agtttgaact gggcaacggc gtgatcttcg tctactacac
     4561 cgccgtcggc gacgtgaacg gcgatggccg gctggacctg gccttccgcc ccaaccccaa
     4621 ccatgcgatg aaggtcctca tcgccacgca gcagggcaac ggcacgttcg cgatcaccag
     4681 caagctgaac gccggaaccg aagaaggggc ggtcctgctc tacgatgtcg acgacgatgg
     4741 caaggatgac ctgctgacgg tcaacggcaa caaccgggtg gacctgttcc tgacgacgca
     4801 catcccctgg gtcaacgacg acgccgggac gacgaagacg gcgtcttaca cggtcagcgg
     4861 gctgggcgcg ggggccgtcg atctggacgt cggcgacgtg aacggcgacg gcgggctgga
     4921 cctcgtcgtc tcgggcggca cgaaactgag cgtctggacc gatcaatcca agctgtggca
     4981 gcccagcgcc agctcgatcc agcccgatct gagcttcgac gcgggcgaat ccgtcgtcgg
     5041 cctcgaggtg atcgatctgg acgtcgacgg ccgcgacgat atcctggtcg gcaccacgac
     5101 cggacgcgtc ctgctcttcc tggcgcggaa tgccgacccg ctcttcgaga acacgccgga
     5161 ccagatctat ggcgtcaacg ccttctccaa cgtgggcgac gtcatcgtgc aggacctcga
     5221 cggggacttg gcctacgacc tgatcgccta cgaggatgtg accggggcga tgacggtctg
     5281 gatggatctg gaggaatcgg gatcggttcc ccagccgccg acgcatgccc ggcactgggc
     5341 catcgtcgaa taaatcgcct gatgcggcgc gtcccggcgg gttcgccccc gccgggatgc
     5401 gctccccgcc cgctgccggt cggcggcgtc aagcggatcg gccccccctt attgtcgctc
     5461 acgccttcgg catgggccgc gcacgggatc ccgacacatc gcgaaggaac ccccaggtgg
     5521 atacagtcgc cgtcaatcac cgccggtgtt accttgagac tttgctctac ggtaatccgg
     5581 aacgaatccc gctgacgccg ggcgagccgc gggaatccac gctgcgccgc tggcacgccg
     5641 agggtttgcc cgaagggatg catcatctcg acgcggcggc ggagcggctc ggcatcgacc
     5701 tgcagcgccc cgcaacgccg ccggtcgatc tcggcgtgga ctttcgcatg atcccctggt
     5761 tcgaggaaaa ggtgctcgaa cacaaagacg gtcactacct ggtccaggac tggatggggg
     5821 ccatcaccga gatctccgat gagtatgatt acacctacat tcgttcggcg aaggatttcg
     5881 tcacgcgcaa atggcaccga ttcccggtcc agacccgcga ggactggcac gagatgcggc
     5941 gccgttacga cccggatacg ccgcagcggc tgccggcgga tttcgccgcg cgctgcgcgc
     6001 ggctgcgcaa gcgcgatgac tacgccggcg tgagcttcaa cggcccctac tggcaactgc
     6061 gcgagtggct cggcttcgag aatctgtcgg tcctgttcct cacgcagccg gactgggtcg
     6121 aggaaatgat cgaattctgg agcggctttg tcagccgcat gctcgagcgg atcctgtcgc
     6181 gcgttcaact cgactatatc tggttcagcg aagacatggc gttcaaggcc cacccgatga
     6241 tcgggcccga catggcccgg cgcttcctct tgccgacctg gcgccgctgg gccgacctgg
     6301 cccgccgtta cggcgtccag atcgtcgacg tcgattccga cggctacatc gaggatctga
     6361 ttccggtctg gatcgaggcc ggggtgaact gttgcgaccc gatcgaggtc gcggcgcaca
     6421 acgacatcct ccggttccgc gagcgcttcg gcacggcgat ggcctacaag ggcgggatcg
     6481 acaagcgggc gatcgcggcc ggcggccgcg cgatcctggc cgagatcaag cgcaacgaac
     6541 cggtcattcg cggcggcggt ttcatcccgg gctgcgacca tggcgtgccc cccgatatct
     6601 cgtgggacaa cttcgtggag tatacgcgcc tgctggccca ggtcaccggt tggctctagg
     6661 cgacccgatt gccccggctg gggaagcggc cgcgatcgcg gcgatcgctc cctggcgccg
     6721 gacatccttc atgcaaaggc aggaacgacg tgacggacat caagatagcg gttatcggcg
     6781 gcggcagcgt caactggatg ccggtgctga tgcgcgacct gtatcagctg gaggaagcaa
     6841 ccggcggcga gatccgcctg tgcgacccga accaggagca tacgcgggcg gtcgtcgcac
     6901 tgctgcaacg ctacaacgag ctgcgcggga agcgctatgc gatctcggtc catgaagacc
     6961 gccgccgggc gctggcgggc gccgatttcg tgctgaccac cttcagcccc ggcgcgatgg
     7021 atgcctttca taacgacctg gagatcccga tcaaatatgg cgtgcgccag ccggtatcga
     7081 tgacggtggg gccgagcggg atctcggcgg ctatccgcac cgtcccggtc gcttacgcga
     7141 tcgtggagga aatggaggag gtctgccccc gggcctggct gctcaacgtc accaacccga
     7201 tgaccaccgt cacccgcgcg atgaacatgg cgagccgcgg cgtcccggtc atcggcatgt
     7261 gccatgaatt ccatgaattc tcccgctatg tcggcgtcat cctggggctg gagcggcccg
     7321 ccgggatcgg cgtcatggac tacctctacc gctggctggc cgagcagggg ctggagtatc
     7381 gcgtcgccgg catcaaccat ttcatctggc tgaccgaagc gcgcctccac ggcgaggacg
     7441 tcatcccgcg catccggcag ttcgcccgcg atcactggga cctccggccg gcggcgggca
     7501 acggcgaacc ccccgatgcc tgggccaatt acagcgcggc caagctggcc ctctgccgga
     7561 cgttcggcta catgccgctg gcgggcgacc ggcacctgat cgagttctgg ccgagcctgt
     7621 gcaaccaggc caacggattc ggcatgcgtt acggcgtgca taaaaccacc gtcgatctgc
     7681 gccgccacat gaaggagcgg caactcgccg agatcgagcg cgtcgcccgc ggcgagcagg
     7741 cggtccactg gcaaacgagc ggcgaggaga tgagcgcgat catccggtcg gtgctgaccg
     7801 gccggccgac aacggcgatc atcaacgcgc ccaaccgggg ccagatcgac aacctgcccg
     7861 acgaagtggt cgtcgagacg ctggcgacgg tctcgtcggc ggcgatcgag ccgatgcgct
     7921 gcggcccgct gcccgccgcg atcgccgccc tctgccgcct gcactgcgct gtccaggagc
     7981 tgaccgtccg cgccgcgctg gcgggggacc gcgacctgct gatcgaagcg ttcagcctcg
     8041 atccgctgtg cgccgccgtc gatttcgccc agatcccccg gctcgtggac gatctgctcg
     8101 aagcgaaccg cccttggctg ccgcggtttt tcgaaacccg cgcgcgccgg ggcggcaatg
     8161 ggcgcgccac gccggtcgcg cacgccgaag aactttccgc cgccgtgccg ggcggtcact
     8221 gagatggaag gctcgatcca ccgcgtgcgc caggtgcttc aggggcggcg gcccgatcgc
     8281 gccccgctgt tcgacctgtt gcgcaacgat gccgtcctga gccacttcgg cggccgccgg
     8341 ctcgatttcg acaacgccca ggaggtcgtc taccgggcgc acggcgcggc gctcgacgcc
     8401 acgcggccgg ccgtccggtt gccgcagccc gagcgctgcg aaaccctgcc ggacggacga
     8461 accatgaagt acctgcgctg gacggcctgg caggcgccgc gcttctatgc aagtcccgaa
     8521 gagttcgcgc aggagcgggc ccgctacgtt gaaacgtttg atgcggcgtg gaacgccgag
     8581 cgtcaacggg cactggatga ggaactggcg ttcttcgcgc gcgaccgcca ccggctcggc
     8641 gatctgttct attttccggc cacgcgcggc gtcgggatcc agccgctcta caccgccatc
     8701 ggcctggagc agttctgcct cttcatgctg tactacccgc aactcatcga cgatttgctc
     8761 gagtgcagcg cgctggaggc gatcgcctgg tgcgagcacc tgccggccga ccatgggatc
     8821 gaggcggtct tcgtcagcga ggacatggcg tcgaagaacg ggccgctgtt cagcctggat
     8881 tggctggatc ggcattactt cccgcgactc cagcgcgtca tcgcggctta tcatcaacgc
     8941 gggattctcg tgatgtttca ttcggatgga aacctgatgc cggtcctgga ccggctggtg
     9001 gcggccggaa tcgacggctt gaacccgctt gaggtgcagg ccggcatgga tccggtccag
     9061 attcacaacc gctatccgaa actgttcctc tccgggggaa ttgacgtctc gaacctgttg
     9121 ccgttcgcca cgcccgacga gattcgcagg gtcgtgcgcc gcacgctgga gggcactggc
     9181 ggccggctgc tggtgggcag cagcaccgag ctgaatcagg aagtgccgct ggagaattac
     9241 ctcgcgctgc acgaaaccgc cctgaacttc acccttgatt gatttgcgcg ggccgggggc
     9301 aatccgcccc tcgcttttcc cgcaaaacca ttgcacaaac aggagtgatc gaagatgacg
     9361 agccagaccc agaccaccga gccgatgcgg ctgaccgagg agcaactgga ggcctatcgg
     9421 cgcgacggct acatcatgat cgaaaacttg gtcacgcccg aggagctgaa gcaactgcat
     9481 catcgccagc gggaatacac gcattgcggt cgcgacgcga gcaagctgag catccaggtc
     9541 gagccccgga tccagcgcgg cgaggtcaag gtggatcatc cgggcgacgg gattcgcaag
     9601 atcgacggcc tgatcgagca cgacgacctg ttccgccggc tggggaccca tcccaacatt
     9661 ctggaaattc tggaacagat tctggggccc gacatcaagc tctaccgcaa cacgctgctg
     9721 ctcaaaccgc cgctggtcgg ctccgccaag gggatgcacc aggattcgcc ctactggccg
     9781 atcgagccga tggaactgtg ctcctgctgg ttcacgctcg acgccgccac accggaaaac
     9841 ggctgcatgg gcgtcatccc cggggcgcac cggcgcggac ccttgccgca tcatcaggtg
     9901 cccgatgatt ttgtgatgga cgaagcgagc tacgatatgc gcgacgtcaa actggtgccg
     9961 atgccggccg gcgcggggtt gttcttccat tcgctgctgc cgcacgtgac ggcgcccaat
    10021 acgtcgcgcc actggcggcg cgcgatcacg atgacctaca tgtcggcccg ctcgcgctac
    10081 accggcgagg gcgaatcccc caacttcatg acggtccgtg ggcgcggcta ccccggttgc
    10141 gtcgggtgaa aagggtggtc ttgaatcacc cgttcgaacc gccgagagct tgccggactc
    10201 gatcctcggc ccgtcccgca gcagcgatgg cagaactgct cgcatcgatc acgctcacga
    10261 gcaagcgcga cgagcgacgg atttcggctc tgtgaacttt ggtgtataca atcggtaaac
    10321 catatgttac ctctgggcct tgaatctctc cgcaatcccg caggctgatc gtacgcgatc
    10381 gggctggtcg ctcccgcgac ggggattgtc cgtctcgatg ccccgattca ccccgcgcgg
    10441 gttcgaccgc cggggagtgg ggattggatg atgtcgaccc gccgctcgac gacccgcttt
    10501 ctgcgcctgc gcatcgcggc ccagtggctc gcgttgtgcg ccacgggagc gctgctgctc
    10561 tgccgcctgg ggcagccgcc gcgatccgat cccggccgcg actcggtcga tgccgacggc
    10621 acgcaggtgg tcgatctgtg gtgcggggtg atcatggggc gttgggacga gtccgcgctc
    10681 caggaggcca ttgaccgctt caatgcccgc caccccgaca ttcgcatcga gccgagcttg
    10741 atcccctaca cggtttacaa cgccaaactg aatctggcga tcgcctgcgg cgccccgccc
    10801 gatatcgtct acaacgccgt gaactttgtc gccggcatgc cccgccacac ggagctggcg
    10861 aacctgctgg tccccgtccc cgattcgatg gtcacccccg aaatgcgccg gggctggggc
    10921 gagacgatcc tcaagaccat cacgcaggat ggccgcatca tgaagttccc cgaccgggcc
    10981 tacctcggcg gcatgatgat ccagggcaac ctggactacc tcgagatggc cggcgtcgac
    11041 gtcaagcact acctcagcga gggctgggat tacgagacgt tcaagcgcga gctgaaacgc
    11101 gttcagcagg tcgtccgcga acgcaccggg cgcgaggtct acgccttcgg gctgaatctg
    11161 gtgaatgtgc cgacgttcct cttccggttt ctgctgccgc cgatcatcgg cgcggacgcg
    11221 accgaacgtt ccttgctggc ctacgacccg gccgcggggc gctacgtcct ggatcccgcc
    11281 atcacgccgg aaattctggc ccaacccctt caactgatgc aggacatgat ccaccgcgac
    11341 cagatgtggg gccgcaagta tctggggatg gactttggcc agatcggcat cgaaatctac
    11401 gaccggctgg agctggtcac cacctttcag gatacgccgg gcaactcgat ctacagccag
    11461 ctcctgcgca accgggactt cgcgcagggc ctgcgcgaag cgcccttgcg cgtcaccaat
    11521 ctgcccgtgc cgacgcccac cggcgacgaa ccgttcctct tcagcgtggg caccgacggc
    11581 tggggcgtga tgcgccaggt gccctacaag ggggatgccc acacaagcgc cgcgctgcag
    11641 gtggcgcgct acctcgcctc ccccgaggtc caggcgctgc gcttccgcta cagcgacggg
    11701 atgcggaaca ccctctggcc cgatcccgac gccgtcgagc ccttcatccg ggatctggag
    11761 gacccgatcc gccaggatcc ctggctggcg tatctgtggg atctttatga gagctggatg
    11821 aaggaggcgg agacctacct cgccgaagaa gaacaattcc ccgatatcca tgaccgccgg
    11881 gcgctgttga acctgcttgg cgtcgcttcc tacggcggtt acttctactc gggccagggc
    11941 aagcagacgc tggagcgcgt gctgttcaac caggtcgaac cgctccaggc cgcgcgcgag
    12001 atgctcgcgg gggcggcccg gttgatcgac gactactacc gccagcgctc gagcggcgac
    12061 ccgcaagacg cagaccgccg ccagatcagc tcggttcgat aagacctcag gaacgaagga
    12121 gtcccattgg gacggcagat cgcagaccgc atcgggaacc gcgtcagtgg ttcgagatcc
    12181 aagccgtggg ttgaaatcgc cgcccggcgg cctttcaacc caccgtcgcc atccgccaat
    12241 taataaaacc gattgatcgg ttcgataaca acgacgtgag acgaaacaaa agaaagggca
    12301 ccgacgttgg aactgaaaac ggacgaactg gttgtgtcgc tggacgagac gcagggcgcg
    12361 ctcgcgatcg ctcggcgtga cggccgcgga tttgccttgc gcgacgccgc tccccgcgtc
    12421 gacggcgatt tcggcgtggt ccgcgcggcg tatcgtcccc ggatccggcg cgaatccttt
    12481 gccgaccccc tcggccaggg ggaacgcctg atcgttgatt ttgccgacct gccgggcggc
    12541 agcgagattc agtggcgcgt gcagctctac cgggagcggc catgggtcag cctgacggcc
    12601 gatctgcgcc ggcccaacgg cctgctgacg cggctctcgc cgctggcggg cagcatcgtc
    12661 gtccccggcg gcaatcgcgc cgagcaggtg cgcgcgttcg tccacagcgg cagccagatg
    12721 ccgtgcgggt cgtgcgacct gggcgccggc ggcatcgcga cggcccatgt gaccgacatc
    12781 gattgcgacc tgcaggccga atccgatgac cgacggttca agagcgagtt cctgacggcg
    12841 ttgtctgagc cgacgaccgg gctgggcatg gcgctgggct tctattccct gcgccgggcg
    12901 tggtgttcga tccggatgca gccggccgac gacgggatca tccacctgct ggcggcgggc
    12961 gagtactacc atcataacct cgcggccgac gccaggcgca tgctgggcga gagcctgctg
    13021 gtggatctga cgcttgatcc cctgaccggg ctggagaact atgcgcgcgc ggtcgaggcg
    13081 caggttcaac cccggttcaa ccgcgaactg gggcgcgggt tccacaatcc atggttctat
    13141 ctgggtaatc agacgcgcgg cgaacacatg ctgcacgacg cggcccggct ggccgaatgc
    13201 atcctctcga agtattacgg catctcggtg gtcatgaccg ggttgtggca gaagcagcac
    13261 cccgtggaag ggcccggcgc cagtcagtac gaggattccc ttaactccgt cggcatgggc
    13321 gaagagctgg tcgacgagaa ccgctatccg gacggcgggc tggagcgcct cgaacgcgaa
    13381 atgcgcgcga ccggaactga agtggcctgc ggcggcttct tcttccaggc cggttcgcgc
    13441 agcaccatcc gccgccgcca tcccgaccgg gtgatcaacg ggcggatcga ttacacccat
    13501 ccggcgtgcc gcgaatggct gcgcggcatc atcgagccgt ttgtccggcg cggcgcgcgc
    13561 tacctgtggg ccgatttcaa cgataacgcg atctacggcg tgcagcacga tccgacgatg
    13621 gtgaagggct tcgaaaacac ccgcgccgga atgcaggtgc tgcgcgacgc gctcggaccc
    13681 gacggcctga tcggcacgta taccagcccc acgatctgct atacgggcat cgtcgaccgc
    13741 atgcggatgg ggctggacat ggtcccgttc aatcattggt cgagcctgcg cgagatcgtg
    13801 cgcaatatgg cggcggccta tttcttccac cagcgcatgt ggatgaacga ccccgatccg
    13861 atcttcgtct gcggcgagtc ggacgatccg gcgacgctgg aggaggcgcg catccgcctc
    13921 gcgctgggga cgatctgcgg cagcgtctgc gccaccggcg agcggttcta caatttcacg
    13981 ccgcaacgga tgcgcctgct cacgaccgcg ctcccgtcgg cggggatcgc cgcgcggccg
    14041 ctcgacctga tgcgccgcga tgtgcccgag gaattcctca ccgtcctcag gcgcgactgg
    14101 gatgcctggc gcgtcgtcag ctacctgaac ctgaacgagg cggaacgaac ctaccccatg
    14161 cagcccgacg cgctggagct ggattcggcg gagggccagc tggtcttcga tttctggacg
    14221 cagcgctgcc tgggcgcgtt gacgccgggc cagacggtga cggttcccgc tcgcacctgc
    14281 cgcgtcctgc tcatccgcga caagaaaccg catccct
//
LOCUS       DAOPCO010000076        14041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4363_length_14041_cov_5.230230, whole genome shotgun sequence.
ACCESSION   DAOPCO010000076 DAOPCO010000000
VERSION     DAOPCO010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4363_length_14041_cov_5.230230"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1633
                     /locus_tag="PLS90_07275"
     CDS             257..1633
                     /locus_tag="PLS90_07275"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HPK02245.1"
                     /translation="MATATNHKAMELEGSLGAFPLADVMRILAMGRMTGILRLESSEH
                     AVELSIKDGRLIGTASPERFLKLGQLLVYNGLINRRDLDEVLLEQREHDTGLPLGELL
                     IDRGLVTREQVEHALRLQVKEEMWELFSWTDGSFRFEHGATPAMAHGLLSLDINRLLE
                     EGEQHMSHWREIAQNLQNPHQVFAVRPDLAGFPEERLRPNVWKLLSLINGRHSLQTLV
                     YMAGLGRFETLCGLDTLLRLNLIMPVEHPVGSMGSQRSDTGDVETQPDVEEAPVTGAQ
                     GRPGALLGLFGRRGGRGEGAEGGPAWQKPRLTPTHGPFFTSVGMACALINQLAQRLLV
                     EPAVKDRIGKSEPIAQLWLQLGMRFPRADLIQVCDGLLDPRPFEHYVQMAGGITRSLA
                     GCHDDSLEALAGVAGGLMELAEEHLGERAGEILRQVARPFIENPEIQHPNDFMPRTWA
                     RQWAEKVL"
     gene            1630..2826
                     /locus_tag="PLS90_07280"
     CDS             1630..2826
                     /locus_tag="PLS90_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02246.1"
                     /translation="MIGSQHLNERPRQDEAPRERQSGFRSILVIDGSHAVQDLARNAL
                     GEAGYRVTTASNGAAVLTYPSLDDIDLLVIDSELAGLTGRETCRLLKQNDGTHEMPVL
                     LLVPEGAVPNRENLELGPVNHFLLKPFDPRQLVRKVEQIFQQKALDQLTLEHMQRSAD
                     RIMTQLAEKQIQAAVERKTQLIIERCIQNITLSVDQYARNEVDSRITALVSDKEQELV
                     KLTVREVANSMVEKLAVAKVEEALQGVLAQQTEAAVRRVTDQTLPGVIRERVREMLGN
                     LLPREIETRLERAAEKMAPELSQQLIGTVEAVVNKTVPRSAREMLPPVVEGQVRAAME
                     TSIPKRVSDLVAVELRTQMTQRIDPAIDAATRRISRGVLMINAAVGFLVLCGLGAMAW
                     LTFFAK"
     gene            complement(2935..5385)
                     /locus_tag="PLS90_07285"
     CDS             complement(2935..5385)
                     /locus_tag="PLS90_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02247.1"
                     /translation="MPGDAYAPDPQIGELAPPAESPPARLRVRGWGWLWLLPPLTLLV
                     ALRVYVLHGDPSDFPLDRNIATALALTRAEPADSRFFPPPPERPEFPAILIGGRLLAA
                     LGADPIVAGRSVTLAAGLLTAIALYLLGRRLSGRPALGLIAVWLHAILPMAIFMERSL
                     LPDALMNAALTWSIVAAAGIRPPDPRRGNGLFPLLARSAAVALLLTAAALAKLPAVFF
                     APVAGFLLPWSVGWRRPAGWIALAVVAGLTWLGVCAWYRLSPFNPLPGLERISQNTNN
                     LLGSVDQLFRPAMLRLVVARITLVLTLPGLLLALAGWIALQSHPGRKGWLNLWALLGF
                     LFVWLTLGANTYWAAAIAPVGMLLAGCALLRLARQPRLAWGLPVLAVAAWLDPARAQI
                     ADYLETRPGLHALAQIGERWFRAPDAPVPNPRPLFYGQVPDDIRYFTGYPGVTLRSWD
                     YDAAVRELEAGRFDFLLSTHMNGERIVGELFSRKPILDFDLRSHILYDTRAQANLGRP
                     VEQAPAGGMALDLEGELIVLAAEVDQTNPAPGEAIELRLTYRKGPRWPRVVPAVLFFS
                     HADSRQTFLPPPRLQGAYFTSWPWPLLQTPDFAQNDTEQMVHRFDLPRAMPEGRYDLF
                     LALARDFVAPAPRGETRPLAITLDVRHREAPAAGAPQPVDWSDALWLIPFFHAEWTPR
                     GIVTLAQLNSQSSRLPQSQVWWAPNLAPGDYLLALRLAARPAGENPADAWPVLKIARP
                     DGSEQTVEIAHEDARTLRLPFSWRGPGDYLHFTLANPLYGTRSRHPFPLYPHDLLYGS
                     RVIELHGATVLPAAAGGE"
     gene            5566..8247
                     /locus_tag="PLS90_07290"
     CDS             5566..8247
                     /locus_tag="PLS90_07290"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK02248.1"
                     /translation="MPLSLPAVRRSACQFIALAVVPWVIVAGQAVAAGSPPRQALRID
                     CQQRGLYRLTGADMQAAGIELSRVDPRRLALVHNQRAVPIHVRTARVDAFGPEDDLFF
                     FSEGGNLDTVPFVNLGEQYAPRTQIFMLHLDGNGHEPLRFEPVELEPPAAGAEPAAVR
                     VVSGRHHYEDNDVWEFFDVNPGVETDFIYWKKLTIPATDRTTNTLVNPVLFPNTVPGA
                     PVRIHGLLVGVSDGEHNVRISVDGKPYHEARWSGPADHAFAFELPADALAAQRGMIRF
                     EALPQSDGPASTTSLIEYEEETNTFRHLNIDVVMVDWFEVEFRQPTAAVNDYNELLVL
                     DETDRANIARARVTGFSSPDILAFDLGAGQVLRGGAYRHEGTPFYAIDVERPTTDTTL
                     VVSTVERARRPQAVRPRPVKGLFAQAPAAELLILTHPDFLEAAQRLAAWKRERGLTTE
                     VVDILDVFTEASGGYALPEALRRYIQHVYQSQTPPRLRYVLLIGDASGISKYTTWCPA
                     YGFIESGKPINENYFATFGEPTDQPVLAVGRLSVRTAGEADQIVNKIMDYESQRHRGI
                     WRARQLMIAANPNWASHMSQYLIRKWVRPEYFARAIQTSMDNPSPDYPDLMSNRLVEG
                     FNAGAIIAAYMGHGGGTIWEMGPTALQSQFTNHLFDQTNVYRLENRERLPLLFALTCY
                     TNNFNHPHVPQTLGEVFMIADGGAIAVLGASGRSDTSENLVFLDCFNRTIQTHAFRRL
                     GEYLLATHRRIPHRQTATRYLLLGDPSLEFNVPRREIELTGQSGAEARELVLDYRLPE
                     AVPLPARLEWLLISEDEDVVAEWSTEADAAEGAVTWRVPEAASAPETWHRVIVYAPAF
                     GADADYVGAWFIAPTQDELNRLQLRTDQTTTVTLEAP"
     gene            8244..8963
                     /gene="rnc"
                     /locus_tag="PLS90_07295"
     CDS             8244..8963
                     /gene="rnc"
                     /locus_tag="PLS90_07295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPK02249.1"
                     /translation="MTAAGPTDEMLARFIERHGLQGLPVEALRLALTHRSHAYEAGLA
                     ADNERLEFLGDAILAAVAAEWLYHLDPAANEGVLSKRRSRIVSRTALGRIALEMELDQ
                     LILLGRGERETGGARRRSTLGSALEAIVGAVYLHAGYAAASEFVRRELLEVQTERAFA
                     PAGDDYKSSLQEWTQQHCRCVPVYHRVGQSGPDHDRIFTVRVEVNGRALAEARGRRIK
                     QAENEAARLALECLTAGEARP"
     gene            8960..10459
                     /locus_tag="PLS90_07300"
     CDS             8960..10459
                     /locus_tag="PLS90_07300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02250.1"
                     /translation="MTARRLRGRIPIGWLALILFLAALAARLAYLNSRGLWGDEFWTV
                     IAIRLPLPGLIENRITRGHLPTYFLLLKPWYGLFGESEWSLRMPSAIASAAAVPLLYL
                     FVARLWNPTAALWAALFLLLNQRAVWTGQEARSFCLILLAAAGSFHALVHALEGKRRW
                     VWAYGLWSLFGALQLAIYPLLFIWQGVVTAGWLIWRRRWRWGWAVAWLIVAAAAAAAY
                     LYLGAHATESKTEGLPGWYPWRLYAKALTEVFWGEFDYVFPRDIFRWVGLALAVGVIA
                     AAGVESRRMSRAGAAEGAAPALVSPVFGWWLGVTWAMAVLVSFALISGLFQVLDDSTR
                     YFTIGTAGAAALSGVAVASISRRVVRRIAGLALLALLAANTAGWYMHPGDQIREAVRL
                     VAEQRRPDEPVFICRDTQAETIADYYGLGTAPIGIPRARIGRFQIERILEEGTAGAPG
                     FWIILYKERHTPLPMVARRWAEGKFELQGGWNFSRSRVQHYVRGSGGGE"
     gene            complement(10559..10894)
                     /locus_tag="PLS90_07305"
     CDS             complement(10559..10894)
                     /locus_tag="PLS90_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02251.1"
                     /translation="MPTPVPNSLVAGAPNAQPASDPAAGAPAPETIDPAAAAGFLVGA
                     AAIIVTLFGILIPLVTWLLAAGLGLITIGCGLFARRPYRRWILLAGAVALIPASILLL
                     VQLLPHYRF"
     gene            complement(11305..12795)
                     /locus_tag="PLS90_07310"
     CDS             complement(11305..12795)
                     /locus_tag="PLS90_07310"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPK02252.1"
                     /translation="MQISVTSRPPAARADDLIVLLDPSAARELFDPARTAALPGWDDL
                     ARHRKTNEVKCPYQASGGAPLLYLPGAIAPYWDDDEKIRILGGKTWDLALDRHARRIV
                     LMLDGRHGPDAAPLAAEGLALRAYRFTKYKKGAEPKREPSVTLVVSDTNRAAVGRAVK
                     RRLTVIESVNAARDLVNEPGSALTPADLEQAARAVARRRGLKITVLNEPELRKQGYNG
                     LLTVGRGGTVPPRMIILRYQPAGRGARKDVHLGLLGKGVTFDTGGISIKPSARMWEMK
                     GDMGGAAAALFAMDAIAQLKPAVAVTAILVTAQNYVDRNSVLPGDIFVAKNGKTVHVD
                     NTDAEGRLILSDGLCRMGEERVTHLVDAATLTGAVVRALGPALTGVLGNDQFADEVIE
                     IAATQGEPCWRLPLVEEYRQWLDIEVADLNNISNPACSLYAGATTAALFLREFVPQGV
                     HWAHLDIAGTSLVTGQYKYFRPGATGVMVRTMAALAERLAARPADG"
     gene            13062..13898
                     /locus_tag="PLS90_07315"
     CDS             13062..13898
                     /locus_tag="PLS90_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPK02253.1"
                     /translation="MQHLAPDPSLEDFRAAVSLTDEEIDLARAALLVACVEYPELAIE
                     TYLDYLNEQAARVRARIEPWDEPLEQVRTLVEVAIRRGGLRGVRMEEGEDYYDPRNSF
                     LNEVIDRHVGIPISLSIVVMGIAERAGIALAGTAMPMHFLVRVLGLKEPLFLDCFNGG
                     QLLTEEDCRRALTLMSQNKVNYHPEVLQAVSNAEILTRLLTNLKLIYFRQSNLAKAVP
                     ILDRLLVLNPGAPPLLRERGLVLFRLGRGRLARSDLERYLASSENPPDAPQIREILGR
                     IV"
BASE COUNT         2311 a         4075 c         5441 g         2214 t
ORIGIN      
        1 gccgagtgac cggtggtgtc gctgcgctca accaccggct attctctggc atcccggcgg
       61 gatgcggggc aagggggggg cctgtcacca tcgaatcaaa tgttcagtcg ggcgggggct
      121 atgccgataa gtctggcggg aaaggagtag cttgcgttcc ggtggatggg ccgtatccgg
      181 cgcccacggg ccgccgcagc gatcagaccg ggccggattt tcagtggctg gcgcagcaag
      241 ggagtaagga gcaggcatgg cgaccgcgac gaatcacaag gcgatggaac tggaggggag
      301 cctgggcgcc ttccccctgg ccgatgtcat gcgcatcctg gcgatgggtc ggatgacggg
      361 gatcctgcgt ctggaatcct cggagcatgc cgttgaactg agcatcaagg acggccggct
      421 gatcgggacg gcctcgccgg agcggtttct caagctgggc caactgctcg tctacaacgg
      481 cctgatcaac cgccgcgacc tggacgaggt gctgctggaa cagcgcgaac acgataccgg
      541 gctgccgctg ggcgagctgt tgatcgaccg cggtctggtc acgcgcgagc aggtggagca
      601 tgcgctccgg ctgcaggtca aggaggagat gtgggaactg ttttcgtgga ccgacggttc
      661 gttccggttt gaacacgggg cgacgccggc aatggcgcat gggctgctgt cgctggacat
      721 caaccggctg ctcgaagagg gcgagcagca catgtcgcat tggcgcgaaa ttgcgcagaa
      781 tttgcagaat ccgcaccagg ttttcgccgt gcgtccggat ttagccggtt ttccggagga
      841 gcgcctgcgc ccgaacgtgt ggaagctact gtcgctgatc aacggccgcc acagcctgca
      901 gacgctggtc tacatggcgg ggctggggcg gttcgagacg ctgtgcgggc tcgatacgct
      961 gctgcggctg aacctgatca tgccggtgga gcatccggtc ggcagcatgg ggtcgcagcg
     1021 gtccgatacg ggcgacgtgg agacgcagcc cgacgtggag gaggctcccg tcacgggggc
     1081 gcaggggcgg ccgggcgcgt tgctgggatt gttcggtcgg cgcggggggc gcggcgaagg
     1141 ggccgagggg ggcccggcgt ggcagaaacc gcgactgacg ccgacccatg ggccgttttt
     1201 cacgagcgtg gggatggcct gcgcgctgat caatcagttg gcgcagcggc tgctggtcga
     1261 gccggcggtg aaggaccgga tcggcaagag cgagccgatc gcgcagttgt ggctgcaact
     1321 cggcatgcga ttcccgcgcg ccgacctgat ccaggtctgc gacgggctgc tcgacccgcg
     1381 gccgttcgag cactacgtgc agatggctgg ggggatcacg cggagtctgg ccgggtgcca
     1441 tgacgacagc ctggaggcgc tggcgggggt cgccggcggg ctgatggaac tggccgagga
     1501 acacctgggc gagcgggcgg gtgaaatcct gcgccaggtg gcgcggccgt ttattgagaa
     1561 tccggagata cagcacccca acgatttcat gccccgcacg tgggcacgcc agtgggcgga
     1621 gaaggtatta tgatcggttc gcagcatctg aatgaacgtc cgcggcagga cgaggcgccg
     1681 cgtgaacggc agtcgggatt tcgctcgatt ctcgtcatcg acggcagcca tgcggtccag
     1741 gacctggcgc gcaatgcgct gggcgaggcc ggctaccgcg tgacgaccgc cagcaacggg
     1801 gcggccgtgc tgacctatcc gagcctggat gatatcgatc tgctcgtgat cgacagcgaa
     1861 ctggcgggct tgacgggccg ggagacctgc cggctgttga agcagaacga cgggacgcac
     1921 gaaatgccag tgctgctgct ggtgcccgag ggggcggtgc cgaaccggga gaatctggag
     1981 ctggggccgg tcaatcactt tctgctcaag ccgttcgatc cgcgccagct cgtgcgcaag
     2041 gtggagcaga tctttcagca gaaggcgctg gaccaactga cgctggagca catgcaacgg
     2101 tcggccgacc ggatcatgac gcaactggcg gagaagcaga tccaggcggc ggtggagcgc
     2161 aagacgcagc tcatcatcga gcgctgcatt cagaacatca cgctgtcggt ggatcagtat
     2221 gcgcgcaatg aagtggattc gcggatcacg gcgctggtca gcgacaagga acaggaactg
     2281 gtgaagctga cggtgcggga agtggccaat tcgatggtcg aaaagctggc cgtggcgaag
     2341 gtggaggaag cgctgcaggg ggtgctggcg cagcagaccg aggcggccgt gcgtcgggtg
     2401 acggaccaga cgctgccggg ggtgatccgg gagcgggtgc gcgagatgct ggggaacctg
     2461 ttgccgcgcg agatcgagac gcggctggag cgggcggcgg agaagatggc gccggaactg
     2521 agccagcagt tgatcggcac ggtggaggcc gtggtgaaca agacggtgcc gcggtcggcg
     2581 cgcgagatgc tcccgccggt ggtggagggt caggtgcgcg ccgcgatgga gacgagcatt
     2641 cccaagcggg tgagcgacct ggtggcggtg gagctgcgca cccagatgac gcagcggatc
     2701 gacccggcga tcgacgccgc gacgcggcgg atcagccgcg gcgtgctgat gatcaatgcg
     2761 gccgtcggtt ttctcgtcct gtgcggactg ggtgcgatgg cctggctgac atttttcgcg
     2821 aagtaatctt tcttcccatt gatccaagcg atgtgcaatc cgtcggatag aaccgatccg
     2881 acggattgcg ggcgtgatcg cacgctggag ggcgtgctgc acgccgtcga gcgtttattc
     2941 gccgccggcc gctgcgggca gcacggtcgc gccgtggagt tcgatgacgc gcgagccata
     3001 aagcaagtcg tgcggataga gcgggaaggg gtgccgcgag cgggtgccat agagcgggtt
     3061 ggcgagcgtg aagtggagat aatcgccggg gccgcgccag gagaacggca ggcgcagcgt
     3121 gcgcgcatcc tcatgggcga tctcgacggt ctgttcggag ccgtcggggc gggcgatttt
     3181 caagacgggc caggcgtcgg cgggattctc gccggcgggg cgggcggcga gccgcagggc
     3241 gagcagatag tcgccgggcg ccagattggg cgcccaccag acctggctct gggggaggcg
     3301 gctgctctgc gaattgagct gggcgagggt cacgatgccg cggggggtcc actcggcatg
     3361 aaagaagggg atgagccaga gggcgtcgct ccaatcgacc ggctgggggg cgccggcggc
     3421 gggggcctcg cggtggcgca cgtccagcgt gatggccagc gggcgggttt cgccccgtgg
     3481 ggccggcgcg acgaagtcgc gggccagggc gaggaacaga tcgtaacgac cttcgggcat
     3541 cgcgcggggg aggtcgaaac ggtggaccat ctgctcggtg tcgttctggg caaagtcggg
     3601 ggtttgcagc aacggccagg gccatgacgt gaaatacgcc ccttgcaaac gggggggcgg
     3661 caggaaggtc tggcggctgt cggcgtggct gaagaagagg accgccggga cgacgcgcgg
     3721 ccagcggggg cccttgcgat aggtgaggcg cagttcgatg gcctcgcccg gcgccgggtt
     3781 ggtctgatcg acttcggcgg cgagcacgat cagttcgcct tcgagatcca gggccatgcc
     3841 gccggcgggc gcctgttcga ccggccggcc gaggttggct tgcgcgcggg tgtcgtaaag
     3901 gatatgggag cggaggtcga aatcgaggat cggcttgcgc gagaagagtt cgccgacgat
     3961 ccgttcgccg ttcatgtggg tgctgaggag aaaatcgaac cgtccggctt cgagctcgcg
     4021 gacggcggcg tcgtagtccc acgagcggag cgtgacgccc ggatagcccg tgaaatagcg
     4081 gatgtcgtcg ggaacctggc cgtaaaagag cgggcggggg ttggggacgg gggcatccgg
     4141 agcgcgaaac cagcgctcgc cgatctgcgc aagggcgtgc aggccgggcc gggtctcgag
     4201 atagtcggcg atctgggcgc gggcggggtc gagccatgcc gccaccgcca ggacgggcag
     4261 cccccaggcc aggcggggct ggcgcgccag gcgcagcagg gcgcagccgg ccagcagcat
     4321 ccccaccggc gcgatggcgg cggcccagta agtgttggcg cccagcgtca gccagacgaa
     4381 gaggaacccg agcagcgccc acaaattgag ccaccccttg cggccggggt gggactggag
     4441 ggcaatccag cccgccagcg cgagcaggag gcccggcagc gtcaggacga gggtgatgcg
     4501 cgcgacgacc aggcggagca tcgcagggcg gaagagctga tcgaccgagc ccagcaggtt
     4561 gttggtgttc tggctgatgc gctcaagccc gggcagggga ttgaacggcg acaggcggta
     4621 ccaggcgcag acgccgagcc aggtgaggcc ggcgaccacc gccagcgcga tccagccggc
     4681 cggccggcgc cagccgaccg accaggggag gaggaatccg gcgacggggg cgaagaaaac
     4741 ggcgggcagt ttggccaggg cggcggcggt cagcagcagg gcgacggccg cggaacgcgc
     4801 gaggaggggg aagaggccgt tgccgcggcg cgggtcgggg gggcggatgc cggcggccgc
     4861 gacgatgctc caggtcagcg cggcgttcat cagcgcgtcg ggcaggagcg agcgctccat
     4921 gaagatcgcc atgggcagga tcgcgtggag ccagacggcg atgaggccga gcgccggccg
     4981 ccccgacaac cgccggccga gcaggtagag tgcgatggcg gtgaggagac cggcggcgag
     5041 cgtgacggag cggccggcga cgatcggatc ggcgccgagg gcggcgagga ggcgcccgcc
     5101 gatgaggatg gcggggaact cggggcgctc cgggggcgga ggaaaaaacc gggagtcggc
     5161 gggctcggcg cgggtcagcg cgagcgccgt cgcgatattg cggtcgagcg gaaagtcgct
     5221 cggatcgccg tggaggacgt agacgcgcaa cgcgacgagc agggtgagcg gggggaggag
     5281 ccagagccag ccccagccgc gcacgcgcag ccgagcgggc ggcgactcgg ccggcggcgc
     5341 cagctcgccg atctgaggat ccggggcgta cgcgtctccg ggcaagggag tgacctcggg
     5401 ggcaggatgt ttcaatcaag caacgatagc gggtggggcg gctgacctca atggaaaggc
     5461 cgatgacacc cggcgggcgg ctggggtaga atcgtcgtcc aaacgcgaga accgcgctcc
     5521 gttgtcgcgt gcactccagc cccggccctt gcgccgcgtc ccacgatgcc tttgtcgctc
     5581 ccggcggtcc gccgctccgc ttgccagttc atcgcgctcg ccgtcgtgcc gtgggtgatc
     5641 gtcgccggcc aggcggttgc cgccgggtcg ccgccgcgcc aggcgctgcg gatcgattgc
     5701 cagcagcggg gtctgtatcg gctgacgggc gccgacatgc aggcggcggg gatcgagttg
     5761 agccgggtgg atccgcggcg gctggcgctg gtgcataacc agagggccgt gccgatccat
     5821 gtgcgcacgg cgcgggtgga tgccttcggc ccggaggacg acctgttttt cttttccgag
     5881 gggggcaatc tcgacacggt tccgttcgtc aatctgggcg agcagtatgc cccgcgcacg
     5941 cagattttca tgctgcatct ggacggcaac ggccacgagc cgctgcgatt cgagccggtc
     6001 gagctggaac cgccggcggc gggggccgag ccggcggcgg tgcgcgtcgt cagcgggcgg
     6061 catcattacg aggataacga cgtctgggag ttcttcgatg tcaatccggg cgtggagacg
     6121 gatttcatct attggaagaa actgacgatt ccggcgaccg accggacgac gaatacgctg
     6181 gtcaacccgg tgctgttccc caataccgtg ccgggcgcgc cggtccgcat ccacggcttg
     6241 ctggtggggg tttccgatgg cgagcacaac gtgcggatct cggtcgatgg caaaccgtat
     6301 cacgaggcgc ggtggtcggg gccggcggat catgcgtttg cgttcgagct gccggccgac
     6361 gcgctcgcgg cgcagcgcgg gatgatccgt ttcgaagccc tgccgcagag cgacggtccc
     6421 gcctcgacga ccagcctgat cgaatacgag gaagagacga acaccttccg gcacctgaac
     6481 atcgacgtgg tgatggtgga ctggttcgag gtggagtttc gccagccgac ggccgcggtg
     6541 aatgactaca acgagctgct ggtgctggac gaaacggacc gggcgaacat cgcgcgcgcg
     6601 cgcgtgaccg ggttcagctc gccggacatt ctggccttcg atctgggggc gggccaggtg
     6661 ctgcgcggcg gcgcgtatcg ccacgagggg acgccgtttt acgcgatcga tgtcgagcgg
     6721 cccaccaccg acacgacgct cgtcgtctca acggtggagc gcgcgcggcg gccgcaggcg
     6781 gtgcgcccgc gcccggtcaa gggtctgttc gcgcaagctc ccgcagccga gctgctgatc
     6841 ctgacgcatc cggattttct cgaggccgcg caacgcctgg cggcgtggaa acgcgagcgc
     6901 ggcttgacca ccgaggtagt cgacatcctc gatgtgttta ccgaggcgag cgggggatat
     6961 gcgctgccgg aagcgctgcg gcggtacatt cagcatgtgt atcagagcca gacgccgccg
     7021 cggctgcgct atgtgctgct gatcggcgat gcctccggga tttccaagta cacgacgtgg
     7081 tgtccggcgt atggtttcat cgagagcggc aagccgatca atgaaaacta cttcgcgacg
     7141 ttcggggagc cgaccgatca gcccgtgctg gcggtggggc ggctgtcggt gcggacggcc
     7201 ggcgaagccg accagatcgt caacaagatc atggattatg aaagccagcg gcatcgcggg
     7261 atctggcgcg cgcggcaact gatgatcgcg gccaatccga actgggcgtc ccacatgtcc
     7321 cagtatctga tcaggaaatg ggtacgtccg gaatacttcg cgcgagcgat ccagacatcg
     7381 atggacaatc cctcgcccga ttacccggac ctgatgagca accggctggt cgaggggttc
     7441 aatgccgggg cgatcatcgc cgcctatatg ggacacggcg gcgggacgat ctgggagatg
     7501 ggtccgacgg cgctccaatc gcaattcacg aatcacctgt tcgatcagac caacgtctac
     7561 cggctggaga atcgcgagcg gctgccgctg ctgttcgcgc tgacctgcta caccaacaac
     7621 ttcaatcatc cgcacgtgcc gcagacgctg ggcgaggtgt tcatgatcgc cgacggcggg
     7681 gcgatcgcgg tgctcggcgc gtccggccga tccgatacga gtgaaaatct cgtgttcctg
     7741 gattgcttca accggacgat ccagacgcat gcgttccgcc ggctgggcga gtatctgctc
     7801 gccactcaca ggcgcattcc gcatcgccag accgcgaccc gatacctgct gctgggcgat
     7861 ccctcgctgg agttcaacgt gccgcgcagg gagatcgaac tgacggggca gagcggcgcc
     7921 gaggcgcgcg aactggttct cgactatcgg ctgccggagg ccgtgccgct gccggccagg
     7981 ctggaatggc tcctgatctc ggaggacgaa gacgttgtgg ccgagtggtc gacggaggcg
     8041 gacgcggcgg agggggccgt gacctggcgc gtgccggagg ccgcgagcgc gcccgagacg
     8101 tggcatcgcg tcatcgttta tgcgccggcc ttcggggccg atgcggacta cgtgggagcg
     8161 tggttcatcg cgcccacgca ggacgagctg aatcggctcc agctgcgcac cgaccagacg
     8221 acgacggtaa cgctggaggc gccatgaccg ccgcggggcc aaccgatgaa atgctggcgc
     8281 ggttcatcga acggcatggg ttgcagggtc tgccggtcga ggcgctgcgg ctggcgttga
     8341 cgcatcgctc gcacgcgtat gaagccggcc tggccgcgga taatgaacgg ctggagttcc
     8401 tgggcgatgc gatcctcgcc gccgtcgccg cggaatggct gtatcacctg gatccggcgg
     8461 ccaacgaagg ggtgttgagc aaacgccgct cgcggatcgt cagccgcacc gcgctgggcc
     8521 ggatcgcgct ggagatggaa ctggaccaac tgatcctgct ggggcggggg gaacgggaaa
     8581 cgggcggggc gcgccgccga tcgacactgg gctcggcgct ggaggcgatc gtcggggcgg
     8641 tctatctgca cgcggggtac gcagcggcaa gcgaattcgt gcggcgcgag ctgctcgagg
     8701 ttcagactga acgggcgttc gccccggcgg gggacgacta caagtcctcg ctgcaggaat
     8761 ggacgcagca gcactgccgc tgcgtgccgg tgtatcatcg cgtcggccag agcgggccgg
     8821 atcacgaccg gatcttcacg gtgcgcgttg aagtcaatgg gcgggcgctg gccgaagccc
     8881 gcggccggcg gatcaagcag gccgagaacg aagcggcgcg cctggcgctg gaatgcctga
     8941 cggcggggga ggcccggccg tgacggcgcg tcggctccgc gggcgcatcc cgatcggctg
     9001 gctggcgctg atcctgttcc tggcggcgct cgcggcgcgg ctggcatatc tgaacagccg
     9061 cgggctgtgg ggcgacgagt tctggaccgt catcgcgatc cggctgccgt tgccgggcct
     9121 gatcgagaac cggatcacgc gcgggcatct gccgacgtat ttcctgctgc tcaagccgtg
     9181 gtacgggctg ttcggcgaga gcgaatggtc gctgcggatg ccgtcggcga tcgcgagcgc
     9241 cgccgccgtg ccgctgctct acctgttcgt cgcccggctg tggaacccca ccgccgcgct
     9301 gtgggcggcg ctgttcctgc ttttgaacca gcgggcggtc tggaccggcc aggaggcgcg
     9361 atcgttctgt ctgatcctgc tggcggcggc cggctcgttc catgcgctcg tgcatgcgct
     9421 ggaaggcaag cggcggtggg tgtgggcgta tggcctctgg tcgctcttcg gcgcgctgca
     9481 actggcgatc tacccgctgc tgttcatctg gcagggagtg gtcaccgccg ggtggctgat
     9541 ctggcggcgg cgctggcgct ggggctgggc ggtcgcctgg ctgatcgtgg cggcggcggc
     9601 cgcggcggca tatctctacc tgggggcgca cgccaccgag tccaaaaccg aggggttgcc
     9661 tggatggtat ccctggcggc tgtatgccaa ggcgctgacc gaggtgttct ggggggaatt
     9721 cgattacgtc ttcccgcgcg acatattccg ctgggtcggg ttggcgctgg cggtaggggt
     9781 gatcgcggcg gccggagtgg agtcgcgccg aatgagccgt gcaggcgcag cggaaggcgc
     9841 agcgcccgcc ctcgtgagcc cggtgttcgg ctggtggctg ggggtgacgt gggccatggc
     9901 ggtgctggtt tcgttcgcgc tgatttcggg tttgtttcag gtacttgacg actcgacgcg
     9961 atacttcacg atcggcacgg cgggcgccgc cgcgttgagc ggggtggcgg tggcgtcgat
    10021 ttcgcggcgc gttgtgcgtc gcatcgcggg gctggcgctg ctcgcgctgt tggcggcgaa
    10081 cacggcgggc tggtacatgc atcccggcga tcagattcgc gaggcggtgc ggctggtggc
    10141 cgaacagcgc cggccggatg aaccggtgtt catctgccgc gatacgcagg ccgagacgat
    10201 cgcggattat tacggcctgg ggacggcgcc gatcggcatt ccgcgcgcgc ggatcggccg
    10261 gttccagatc gagaggattc tggaagaggg gacggcgggg gcgccggggt tctggatcat
    10321 cctctacaag gaacggcaca cgccgctgcc gatggtcgcc cggcgctggg ccgaagggaa
    10381 gtttgagctg caaggggggt ggaacttcag ccgcagccgg gttcagcatt acgtgcgcgg
    10441 gtcggggggc ggtgagtgaa gcgcggggtg gcggcttgag agtcgaaagc cgataattgg
    10501 ggcggggctt tgggagcgat cacgaggacg agggacgagt gacggaaatt gggttggtct
    10561 agaatcggta gtggggcagg agctggacga ggagcaggat agaagcgggg atgagcgcga
    10621 cggcgccggc gagcaggatc cagcgccggt agggtcgccg ggcgaacagg ccgcagccga
    10681 tcgtgatcag gccgagcccg gcggcgagca gccaggtgac gagcgggatg aggatgccga
    10741 agagcgtgac gatgatcgcg gcggcgccga cgaggaagcc ggcggcggcg gcgggatcga
    10801 tcgtttcggg cgcgggggcg ccggcggcgg ggtctgaggc cggttgggcg ttcggcgcgc
    10861 cggcgacgag cgaattgggt acgggcgttg gcatgggggc gcctccacga aggcgttgac
    10921 gggcgtggcc ggggaacggc aagcccttcg atgggattga tcgtagcgcg gtgcgggcgg
    10981 aaaacacaat aaggaaatct gaatatctga taattgaatt tcgaaactcg aatttcgaaa
    11041 tttgatacaa gttcgaattt tcgaaaaagc ccagatgacg aaaaagggcg tcatgcggag
    11101 agggtgagga tgggacgctc ttccgtgggt gagagtcgcc ccctttgggg aggggggggc
    11161 acacgtcggg gtccccaggt tccgctgtgc gctacctggg ctatccgaat cccgccccgc
    11221 tggggcgggt ccgcggacgc aatacccgag tttcgaaatt cgaatcgggt atttcgagca
    11281 tccgggacga accggttgcg ggactcagcc gtccgccggg cgggcggcca atcgttcggc
    11341 cagcgccgcc atcgtccgaa ccatcacgcc ggttgcgccc gggcggaagt atttatactg
    11401 gccggtgacg agcgacgtgc cggcgatatc cagatgggcc cagtggacgc cctgcgggac
    11461 gaactcgcgg aggaacaggg cggccgtggt ggcgccggca tacagcgaac acgcgggatt
    11521 ggagatgttg ttcaggtccg cgacctcgat atcgagccac tggcggtatt cctcgacgag
    11581 ggggaggcgc cagcacggct cgccctgcgt ggcggcgatc tcaatgacct cgtcggcgaa
    11641 ctggtcgttg cccaggacgc cggtcagggc gggcccgagg gcgcggacga cggcgccggt
    11701 cagcgtggcg gcgtcgacga gatgcgtcac gcgctcctcg cccatgcggc acaggccgtc
    11761 gctcaggatg agtcgcccct cggcgtcggt gttgtcgaca tggacggtct tgccgttttt
    11821 tgcgacgaag atgtcgccgg gcaggacgga gttgcgatcg acatagttct gcgcggtgac
    11881 gaggatcgcg gtgacggcca cggccggttt gagctgcgcg atggcgtcca tggcaaacag
    11941 cgcggcggcg gcgccgccca tgtcgccctt catctcccac atgcgggccg agggcttgat
    12001 gctgatgccg ccggtatcga acgtgacgcc cttgcccagc aggcccagat ggacatcctt
    12061 gcgggcgccg cggccggccg gctggtagcg caggatgatc atccgggggg ggacggtgcc
    12121 gccgcggccg acggtgagca ggccgttgta gccctgcttc cgaagttcgg gctcgttgag
    12181 gacggtgatc ttgagtccgc ggcggcgggc gacggcgcgg gcggcctgtt caagatcggc
    12241 cggggtgagg gccgacccgg gctcgttgac cagatcgcgc gcggcattga ccgactcgat
    12301 gacggtgagc cggcgcttga cggcgcgacc gacggcggcg cggtttgtgt cgctcacgac
    12361 cagggtgact gagggttcgc gtttcggttc ggcgcccttc ttgtatttcg tgaagcgata
    12421 ggcgcgcagg gcgaggccct cggcggccag cggggcggcg tcgggaccat ggcggccgtc
    12481 gagcatgagg acgatgcgcc gcgcgtggcg atccagcgcc aggtcccagg tcttgccgcc
    12541 gagaatgcga attttctcgt cgtcatccca atacggcgcg atggcgccgg gcagatagag
    12601 cagcggcgcc ccgccggatg cctgataggg gcacttgacc tcattggtct tgcgatggcg
    12661 ggcgaggtcg tcccatcccg gcagggcggc ggtccgggcg gggtcaaaga gctcgcgcgc
    12721 ggcggaggga tcaagcagga cgatcaaatc atcggcacgg gcggccggcg gccgggaggt
    12781 cacggagatc tgcatcagac ggggaagctc cttgcgatgg ggtgcaggcg tggcgcttgc
    12841 gagccggtat tttgccgaac cggcgcggga acgtcaacga aggggcggga ggatggaggg
    12901 ctttgcgggc gattacgagg aacggggacg agggctgagg gacgcaagcg ggcaaattga
    12961 ctcgcccacg gccgggggtt gggtgtattc tgtagcgtgg tttcaacgct gattctcgtc
    13021 gtgggacgac gagcggcagc caatgaggat actggagcgc gatgcagcat ttggctcccg
    13081 acccctcgct cgaggatttt cgcgcggcgg tctcgctgac cgatgaggag atcgatctgg
    13141 cgcgcgccgc gctgctggtc gcgtgcgtcg aataccccga gctggcgatc gaaacgtatc
    13201 tggactatct gaacgagcag gcggcgcggg tgcgggcgcg gatcgagccg tgggacgagc
    13261 cgctggagca ggtgcggacg ctggtggagg tggcgatccg gcgcggcggt ctgcgcgggg
    13321 tgcggatgga ggagggcgag gattactacg atccgcgcaa ttccttcctg aatgaggtga
    13381 tcgatcggca cgtcgggatt ccgatttcgc tctcgatcgt ggtgatgggg atagccgagc
    13441 gggcggggat cgcgctggcg gggacggcga tgccgatgca ttttctggtg cgcgtgctcg
    13501 ggctgaagga accgctgttc ctggactgct tcaacggggg ccagctcctg accgaagagg
    13561 actgccggcg cgccctgacg ctgatgtcgc agaacaaggt caactatcac cccgaagtgc
    13621 tgcaagcggt gtcgaacgcg gaaatcctga cgcggctgct gacgaatctg aagctgatct
    13681 atttccgcca gtcgaatctg gccaaggcgg tgccgattct ggaccggctg ctggtgctga
    13741 acccgggggc gccgccgctg ttgcgcgagc gcgggctggt gctgttccgg ctcgggcggg
    13801 ggcggctggc gcgcagcgat ctggagcgct acctggccag cagcgagaat ccgcccgacg
    13861 cgccgcagat ccgggaaatc ctggggcgga tcgtctgaca cacccggcgc gcggggatca
    13921 tggcgcgtcg ggcatgacgc cgtaataatc gacatccacg atttcgtcgc ggccgagcgt
    13981 ctggccggcc tgcatcgcct gttgcagcgc cgacttgcgc gccgggtcga tccagaagac
    14041 c
//
LOCUS       DAOPCO010000077        13905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4408_length_13905_cov_4.886643, whole genome shotgun sequence.
ACCESSION   DAOPCO010000077 DAOPCO010000000
VERSION     DAOPCO010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13905
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4408_length_13905_cov_4.886643"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLS90_07320"
     CDS             <1..1398
                     /locus_tag="PLS90_07320"
                     /inference="COORDINATES: protein motif:HMM:NF001964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPK02254.1"
                     /translation="VLNFEYQGHRINLLDTPGHKDFSEDTYRVLTAVDSVVMVIDAAK
                     GVESQTRKLFEVCRLRGIPIFTFINKMDRPGREPLELLDDLESVLGISACPMNWPLGT
                     GFEFKGVWDRQARAAWFFERTPSGMFRAPVQTGGWDDPEIRARLDEEAWRKVKEEMEI
                     LEGAGAAFDHARVLAGELTPVYFGSALNSFGVQNLLDSFVALSTGPTARISHGSAIAP
                     TAEPFSAFVFKIQANMDPNHRDRVAFIRVVSGKFERDMTARHVQSGKTVRLSNSSKLF
                     GRERETVDVAYGGDIVGIVGANYLAIGDTLGSRPDIVYDEIPHFPAECFAFLHNPSPS
                     NFKRFRTGLAQLLQEGVVHSFELEHGPRNAPLLAAVGPLQFDIVRYRLETEYGAASRL
                     ESAPWERARWARFDAVDRDRIKTAAGVAWARDAEGRPVLLFPNEWHIDYFARTNDFVS
                     LSEYPIDPVEEAISA"
     assembly_gap    1447..1546
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1547..2541)
                     /gene="ilvC"
                     /locus_tag="PLS90_07325"
     CDS             complement(<1547..2541)
                     /gene="ilvC"
                     /locus_tag="PLS90_07325"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPK02255.1"
                     /translation="MPKLYYDQDADLGLLKGKTVGIIGYGSQGHAQAQNLRDSGVKVL
                     IGQRPGGPNYDLAVEHGFQPVSAAEVAQQADIIQILIPDELQSKLYENDLKPHLKPGK
                     ALVFSHGFAIHFSQIVPPSDVDVWMVAPKGPGHLVRRTFTEGGGVPCLVAVHQNASGK
                     ALELALAQAKGVGGTRAGVYETSFREEVETDLFGEQVVLCGGLTALIQAGFETLVEAG
                     YSPEMAYFECCHEVKLIVDLIYEGGIANMRYSISNTAEYGDLTRGPRIVTEQTKAEMK
                     KILNEIQTGQFAREWVLEAQANFASFRARRRLGQEHPIEKVGAEVRSNMPWLKRK"
     gene            complement(2783..3283)
                     /locus_tag="PLS90_07330"
     CDS             complement(2783..3283)
                     /locus_tag="PLS90_07330"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK02256.1"
                     /translation="MTEQENTPQEQQEPTPAAHEATAESAAASKAAQFHGRLVDLYRQ
                     ALTQDGRKAYDRWGLALFHSLPDEEAQAQRENLGLTQGDALDHYNTGCLLAARQDYAG
                     AVAAFERALALNPQMREAAYNKALALELSGKTAEARKAWQSWLEQAPDGEEAAEVRQH
                     LAELAG"
     gene            complement(3457..3825)
                     /locus_tag="PLS90_07335"
     CDS             complement(3457..3825)
                     /locus_tag="PLS90_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02257.1"
                     /translation="MATKVLLADDEEDVKVVLKMFLESKGYDIVTAYDGLDAIDQART
                     HKPDVILLDIMMPLVDGFEVCRKLKGDPETAEIPIIMLSAASHAESVQKGLDAGAAEY
                     LIKPFEPEQLVKVLEDVVKA"
     gene            complement(3986..5620)
                     /locus_tag="PLS90_07340"
     CDS             complement(3986..5620)
                     /locus_tag="PLS90_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02258.1"
                     /translation="MGSERYLALFRELTPLIGSTDPEEICVRAGELIARLLGVEACSL
                     MLVDGAEGDLRLVAATHIAPEHWPRVRTALDEGILGEVVRADQAVLIVGAAEFRRIFG
                     RAPEARYRTPSCVIVPLPIQGRPGGVINVAHPTEDRLFHQRDVELLRAAAGLIGAALA
                     NAFQHQETLKLQQNLEDIFSSLHVGIVTVDENGRIRHTNQRARRMFGHTSRECLAELE
                     DLLPEEVASLCRRLIEQLGSGQEMVQEPVRTTVNAQPLKLEVTVSSLSGIAGQGNDAL
                     VLFHDIGQEEEVRRLRESEKMKRGFLAIISHELRTPISVINGSLPMIDVNGGREVTNE
                     TLSQVHRLLRKNSQRLCDVVNAILDVTEIESGTLQLSLRQVDLPSMIEDILAVQAESA
                     SLKRVTWDVRFDSNLSRVIADKRRLRQVLTEMVANAIKFSHSDQKISILTRVQPPWVE
                     IDISNRGVRIEPECRRTIFEKFQQGDQSTTRVAGGCGLGLFLARNILQLHGGSIELLE
                     TEGDLTTFRIRLPLPTDEEISAHEMTDAHRQPTCAT"
     gene            complement(5911..7899)
                     /locus_tag="PLS90_07345"
     CDS             complement(5911..7899)
                     /locus_tag="PLS90_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02259.1"
                     /translation="MRRAALALALVLTMPFPLGAAPAALQDTVSTAPLSRQAAELMRD
                     WLAGQDPTARPVQPELEAWRDYIATTMNLEPARQEEALRELSETTDDPLLRGVILRTI
                     SENPLFQSRQAEFIRRYGFYANWFNRLVFSGWRAVQGNVQAGLQLGVDAIFSFFRPSA
                     ADALDRRAYALMREAETLRQSGAANQEALRELEQAVDRALAEADMERAQWALEAGRPE
                     VAAFYARQASGLQPEWSGAAALARTAEAEAARLRREAVASAQVGYPDRAPPVDLTPPD
                     HLRALLLGQPTTGTAALAAAAVAALPKPGQSQSTRMRPWARLIEEHAQAPPAERRWLE
                     ALRASAQHNPDVRLERAEDRRRGAVWRFIFVGPEHARERTYKAATWMTTALDAMENVG
                     LFYVFEVIGRAGYALFARPVPAEEVADAQAAWLRLAQEPYGAEGQRIARELAEYYLDQ
                     QRFEQARELEQSAGAQPGRLRKLNRAEARWLLTQSQQLPDGPLREAAREEARRLAPEL
                     KPDKTPVRKAAAEPVEIESNWDRVSDLVGVAAPAHLPGNSDWFDRNPVNGEASGPVYF
                     ETRPTDADVTIRYPVRFGRRLRIHEATVRRAALPPQVSRWLEWTLAERSEAAETIEQL
                     DRLPIPFEIEGGAGPSGIDLYPRLLPIESKPGELDLYR"
     gene            complement(7896..9641)
                     /locus_tag="PLS90_07350"
     CDS             complement(7896..9641)
                     /locus_tag="PLS90_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02260.1"
                     /translation="MRSLTANEKLLVVLTVSALLWRGLFFPVTQGAYTDGILQIDAFR
                     QGLTFWPPLYGLLSRLFVWLPGVGLEGSARIVSWLAGAAVVPVLFAMALRLFGRRAAI
                     YAAVVWLVSPMALRWSVQVMTDMTATALWSAALGALLLAYEQALPELFPSPPRGAPSP
                     RRTFEWLLIGSLLGTLATLTRYQGILLLPPMALASAHLDRVLRHRNPSGTERRTWWGL
                     LPWVAVPLWLLRQGPGPLENHFRQIGERGSQLDFFQTLYGVYWLQFEEFLRALPVFLT
                     LGITGFLLYGLLRTRWETARLRWFGWLALYLMLAILALQSVFSSFQARYLLPLLPLVC
                     LFAGHGFATLDRHVKRRPVYWAVAGAALGYSLLMALLVGVWQGSPFLDLKQAALYINA
                     QEDLRADARVYTNELYNADIQGAKLRFWLNDREVYLEPATAALMQGSLRDNPPPREGD
                     LLVLSSAYGGGIAPYRSLIENLQQQGMARVRRRFPHRLIPFLPDIMAEPGTHQNPLAL
                     VLRYRMQVFETTVMEITAAPQRRLENPPAPPLPEPQLPGPNRETLQRIESLSREIDAL
                     RGAADRPGTATEPGS"
     gene            complement(9638..10201)
                     /locus_tag="PLS90_07355"
     CDS             complement(9638..10201)
                     /locus_tag="PLS90_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02261.1"
                     /translation="MTRLLLLTLLYLFIALPIEVIVLPTLQGFSPHGWHFLYHWPLLA
                     LPALLLGFLRGEIAGMLMGVVAALLVCFSQPPNNSLGAMIVGLSLTGYLAGQAARRGR
                     FTSFASRWLLLLGLLMLNQFVSGLVRRLFWVYAPLAMPWEHPFGLILTSLLGVLILKW
                     AAPRFKLALSADDDRITPPAQGLRRTR"
     gene            complement(10198..11286)
                     /gene="mreC"
                     /locus_tag="PLS90_07360"
     CDS             complement(10198..11286)
                     /gene="mreC"
                     /locus_tag="PLS90_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPK02262.1"
                     /translation="MAWRSRLRLFVLGAFVVAGLTVCTLERFRVDETYRVWGRVRGVL
                     ARYLEPIQVRTTGVIKQIQRIDALWAAEEENRRLRSELSQARLENQQLIERVGRLERL
                     TGLGRWAAPPQLQFLLADVTGLLARDRGAVLMINRGAEDGIAPRDPVVALGGLVGIVQ
                     MVDRDGAQVLTVRDPLFAAGAVTAQTRSRGVVRGSGLEAPLEFMPENETTPIPEGEML
                     ITSGFENSVYPKGIEIGRITARGVNAEGYPYGLVEPSVRLDAVEEVLLVVPAHRPARG
                     AIATTATLGQYWIGMPTPRSEQPLMGPPPPPRDEATTEPLIFRFDAPATSTLRADEPA
                     TGGLAHDDAPSTLGLGVSPLEPPEAPDA"
     gene            11539..12456
                     /locus_tag="PLS90_07365"
     CDS             11539..12456
                     /locus_tag="PLS90_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HPK02263.1"
                     /translation="MDEREDGSVPAAPDAENEVGGARDGPVYGRARAGSPAVPPPGWD
                     PESSAGSAAPAAAESAVWTYPGAGQTPWGEPIPPRDGPAWEWRRELGTVRAAWLTVRE
                     IFTSPDQAFSRAIRDGGLGAPFQFYILMGYVFGILGFVISWPIQLLAERVMGGMFPGT
                     SGGVAAEWPPFMRFLDNGSSFGVALLIQIVLLPFTLTAILFVGSAILHVFLKLLGGGR
                     AGFEASFRAVAYAQGSVTPFQIVPFVGPMVAMVLGIIFLIIGLARMHETATWRAVLAV
                     LLPFMLITVFAVLAAVVVILALYGGARGF"
     gene            13071..>13905
                     /locus_tag="PLS90_07370"
     CDS             13071..>13905
                     /locus_tag="PLS90_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK02264.1"
                     /translation="MTTMGQQIPQALQELIESSVSLLPETTLEILRLAPDYSTTNLTL
                     QRMLEHDRQHDLADRVLALANSVEFHGLSTIRSIPDAIARIGRLRLFNLVLTSVLGGV
                     YRRTDEYARLLWRHALACGYVCAGLAERFKVAESELAFAAGVLHDIGKFIIYRRLPHL
                     YRQFWIGKSHAFRLIELEARTFPDFAHARVGAAALRRMGLLGVLVEAAALHHAFESGP
                     AKASNPGLVSVVSLGNVLVNNVGLERKVCEWKAIEELYCARHIGAQGRTLDEAIAALP
                     GL"
BASE COUNT         2290 a         4670 c         4445 g         2400 t
ORIGIN      
        1 gttctcaatt tcgagtacca gggccaccgc atcaacctgc tggacacgcc cggccacaag
       61 gacttctccg aggataccta ccgggtgctg accgccgtcg actcggtggt gatggtcatc
      121 gacgcggcga agggggtgga gagccagacg cgcaagctgt tcgaggtctg ccgtctgcgg
      181 ggcatcccga ttttcacctt catcaacaag atggaccgcc ccggccgtga gccgctcgaa
      241 ctgctcgacg acctggagag cgtgctgggc atctccgcct gtccgatgaa ctggccgctc
      301 gggaccggat tcgaattcaa gggggtctgg gaccggcaag cccgcgccgc ctggttcttt
      361 gagcggaccc ccagcggcat gttccgcgcc ccggtccaga ccggcggatg ggatgacccc
      421 gaaatccgcg cgcggctcga cgaagaggcc tggcggaagg tgaaggagga gatggagatc
      481 ctggagggcg cgggcgcggc gttcgatcac gcgcgcgtcc tggccggcga gctcacgccg
      541 gtgtatttcg gcagcgcact caacagtttc ggcgtgcaga atctgctcga ttcgttcgtc
      601 gcgctgtcca cggggccgac cgcgcggatc agccatggga gcgccatcgc gccgactgcc
      661 gaaccctttt cggcgttcgt cttcaagatc caggcgaaca tggaccccaa ccaccgcgac
      721 cgcgttgcgt ttattcgagt tgtctcgggc aagttcgagc gcgacatgac cgcccgccac
      781 gttcagtcgg gcaagaccgt ccgtctttcc aactccagca agctgttcgg ccgcgagcgc
      841 gaaaccgtgg acgtcgccta tgggggcgac attgtcggga ttgtcggcgc caactatctg
      901 gccatcggcg acacgctggg cagccgaccg gatattgttt acgacgagat cccgcatttt
      961 ccggccgaat gcttcgcctt cctgcacaac ccgtcgccgt ccaatttcaa gcggttccgc
     1021 accggcctgg cgcagctcct gcaggagggg gtcgtgcatt cgttcgaact ggagcacggg
     1081 ccgcgcaacg cgccgctgct ggcggcggtt gggccgctgc agttcgacat cgtgcgctac
     1141 cggcttgaga ccgaatacgg cgcggccagc cgtctggaaa gcgcgccgtg ggaacgggcg
     1201 cgctgggcgc ggttcgacgc cgtggatcgt gatcggatca aaacggccgc gggcgtcgcc
     1261 tgggcgcggg acgccgaagg ccgccccgtc ctcctttttc ccaacgagtg gcacatcgac
     1321 tactttgccc gtacgaacga tttcgtcagc ttgagcgaat acccgatcga cccggttgag
     1381 gaagcaattt cggcctgagg ccttacctca gcctcaaata atcctcaatc gaatagccgg
     1441 cgtcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntact tccgtttcag
     1561 ccacggcatg ttgctgcgga cctcggcgcc gaccttttcg atcgggtgct cctggccgag
     1621 ccgacgccgc gcccggaaac tcgcgaaatt cgcctgcgcc tccagcaccc attcccgggc
     1681 gaactgcccg gtctgaattt cgttgaggat cttcttcatt tccgccttcg tctgctcggt
     1741 cacgatccgc ggtccgcgcg tcaggtcgcc gtactccgcc gtgttcgaga tcgaatagcg
     1801 catattggcg atgccgcctt cgtagatcaa atcgacgatc agcttcactt cgtggcagca
     1861 ttcgaagtag gccatctccg ggctgtagcc ggcctcgacc agcgtttcaa accccgcctg
     1921 gatcagcgcc gtcaggccgc cgcacagcac cacctgctcg ccgaacagat cggtctcgac
     1981 ctcttcgcga aagctcgtct cgtagacgcc ggcgcgcgtg ccgccgacgc ctttggcctg
     2041 ggccagcgcc agttccagcg ccttgcccga agcgttctgg tgaacggcca cgaggcaagg
     2101 cacgccgccc ccctcggtga acgtgcgccg caccaggtgg cccggcccct tgggcgccac
     2161 catccacacg tcgacgtcgc tgggcggcac gatctgcgag aaatgaatgg caaaaccgtg
     2221 gctgaaaacg agcgccttgc cgggcttcag gtgcggtttg agatcatttt catacagctt
     2281 gctctgcagt tcgtcgggga tcaggatctg gatgatgtcg gcctgttgcg ccacttccgc
     2341 ggcggagacg ggctgaaatc catgttccac ggccagatcg taattgggcc ctcccgggcg
     2401 ctggccgatc aggaccttca ccccgctgtc acgcagattc tgcgcctggg cgtgtccctg
     2461 gctgccgtat ccgataatgc ccaccgtctt gcccttgagc agccccaggt cggcatcctg
     2521 atcgtagtaa agctttggca tggtcgtccc ttccgtcacg gctgattggg cggcgcggca
     2581 atcggcgggg ggagagatcg tccccggcat ggtcgaaccc ggaccgctcc gaaaccccat
     2641 acgctaggga gggccgggac gaagtgcaag gtaatttgac ggaaaaatcg actggtctgg
     2701 aattctcaag cggacaggcg aggggcgggt cgctgtgtcg atcctcggtt ctcgaatttc
     2761 gtcgcggttt tcgccgctta cgctatccgg ccagttccgc caggtgctgg cgcacctcgg
     2821 cggcctcttc gccgtcgggg gcctgctcca gccagctctg ccaggccttg cgggcctcgg
     2881 cggttttgcc gctcaattct aacgccagcg ccttgttgta ggcggcttcg cgcatctgcg
     2941 ggttgagcgc cagcgcgcgc tcgaaggcgg cgacagcccc cgcgtagtcc tggcgcgcgg
     3001 ccagcaggca gccggtgttg tagtgatcca gcgcgtcccc ctgcgtcagg ccgaggtttt
     3061 cgcgctgggc ctgcgcctcc tcatcgggga gcgaatggaa caacgccagt ccccagcgat
     3121 cataggcttt gcgcccatcc tgcgtcaggg cctggcggta gaggtcaacg agtcggccat
     3181 ggaactgggc agccttgctt gccgccgccg actccgcggt tgcttcgtgg gcggccgggg
     3241 tcggttcctg ctgctcctgg ggcgtatttt cctgctcggt catgcgccgc tatccttcca
     3301 cttgttccta tcgggccgcg cggggaaggt cccggcggat tgcggttgtc ccgccgacat
     3361 ccggcggaac atgacaaacg cactatggta gggtttcggg cagggaatga caaggggagt
     3421 tcgaagcgcg ggtaaacgcg caccgcgggg gcgccgtcag gccttcacga cgtcctccag
     3481 aaccttgacg agctgctccg gctcgaaggg cttgatcaga tactcggcgg ccccggcgtc
     3541 gagccccttc tggaccgatt cggcgtggga ggcggccgag agcatgatga tcgggatctc
     3601 cgccgtttcg ggatccccct tcagcttgcg gcacacctcg aagccgtcca ccagcggcat
     3661 catgatgtcc aggaggatca cgtcgggctt gtgcgtgcgc gcctggtcga tcgcatccag
     3721 tccgtcgtag gccgtcacga tgtcgtaccc cttggactcc aggaacattt tcaggacaac
     3781 cttgacatct tcttcgtcat cggccagcag gaccttcgta gccatgggcg ttgctgatcc
     3841 tccctgtgga ttcgtgggcg tcgccgccgt gcgcggggaa ccttgggcgc cgggcgcaga
     3901 cggcacggca ggtcgaaatc gcccccaagt tctagcagac gccgccagcc ctcgcgagtg
     3961 gaatgtgccg ccgccggcgg agcgatcatg tcgcgcaggt gggctggcga tgggcgtccg
     4021 tcatttcatg ggcggagatc tcttcatccg tcggcaatgg cagccggatg cggaaggtgg
     4081 tcaaatcccc ctcggtctcc agcagctcga tgctgccgcc gtggagctgc aggatgttgc
     4141 gcgccaggaa caaccccagt ccgcagccgc cggccacgcg cgtggtcgat tgatccccct
     4201 gctggaactt ctcgaagatg gtccggcgac attccggctc gatgcgcacc ccccgattgc
     4261 tgatgtcgat ttcaacccac ggcggctgca cgcgcgtcag gatgctgatc ttctggtccg
     4321 agtgcgaaaa cttgatcgcg ttggccacca tttcggtcag gacctggcgc agccggcgct
     4381 tgtcggcgat cacccgcgac aggttggaat caaagcgcac gtcccatgtc acgcgtttga
     4441 gcgacgcgct ttcggcctgc accgccagaa tgtcctcgat catcgacggc agatccacct
     4501 ggcgcaacga aagctgcagc gtgccgcttt cgatctccgt cacgtccagg atcgcattga
     4561 ccacgtcgca caggcgctgc gaattcttgc gcagcaggcg gtggacctgg ctcagcgtct
     4621 cgttcgtcac ctcgcgtccg ccgttgacgt cgatcatcgg cagcgatccg ttgatcaccg
     4681 agatcggcgt gcgcagctca tggctgatga tcgccaggaa gccccgtttc atcttttccg
     4741 actcgcgcag gcgccgcacc tcctcctcct gcccgatgtc gtggaacaag accaatgcgt
     4801 cattgccctg accggcgatg ccgctcaggc tgctgaccgt cacttcgagc ttgagcggct
     4861 gggcgttcac cgtcgtccgc accggctcct gcaccatctc ctggcccgag ccgagttgct
     4921 cgatcaggcg ccggcagagg ctcgcgactt cctcgggcag cagatcctca agttccgcca
     4981 gacattcgcg gctggtatgg ccgaacatcc gccgcgcccg ctgattggtg tgccggatcc
     5041 ggccgttctc gtcgacggtg acgatcccca catgcaggct gctgaaaata tcttccaggt
     5101 tctgctgcag cttgagggtt tcctggtgct ggaaggcgtt ggcgagggcg gcgccgatca
     5161 gtccggccgc cgcgcgcagc aactccacgt cgcgctggtg aaacagccgg tcctcggtgg
     5221 gatgcgccac gttgatcacg ccgccgggac gcccctggat cggcaatggc acgatcacgc
     5281 agctcggcgt gcgatagcgg gcctcgggcg cccggccgaa gatccggcga aattccgcgg
     5341 cgccgacgat cagcacggcc tgatccgcgc ggaccacttc gcccaggatc ccctcgtcca
     5401 gcgcggtgcg caccctgggc caatgttccg gggcaatatg cgtcgccgcc accagccgca
     5461 gatcgccttc ggcgccgtcc accagcatca gcgaacaggc ctcgaccccc agcaggcgcg
     5521 caatcaactc ccccgcccga acacaaatct cctcggggtc ggtgctgccg atcagcggcg
     5581 tcagctcgcg gaagagcgcg aggtaacgct ccgaacccaa gctctcgctc gggatcagaa
     5641 cgttgttttc gcgaaggatt tcagatgact tccggaccac aggctttcct tcgacccctg
     5701 gactgagacg gcagtccggc tggcgcccga tccgcacccc gccatctcca ttaacggcac
     5761 gtgtcgcgtt ccgctttagc cacttgtcgc cccgataggc tctgctcctg aggcgatgga
     5821 ttcgtcaggc gcacgcttgt cagcccgctc tgatcaatat actggatttc acgtccgctg
     5881 agaagttttt atcgtccaac accgccaatt tcaacggtac agatccagtt cgcccggctt
     5941 tgattcgatc ggcagaagcc tggggtacag gtcgattccc gacgggccgg ccccgccttc
     6001 gatttcgaag gggatgggca gccgatccaa ctgctcaatc gtctcggccg cctcggagcg
     6061 ctcggcgagc gtccattcca gccagcggct cacctgggga ggcaatgcgg cccgccgcac
     6121 ggtcgcctcg tgaatccgca gccggcggcc gaatcggacc ggatagcgga tcgtcacgtc
     6181 ggcgtcggta gggcgggtct cgaaatagac cgggccgctc gcttcgccat tgaccgggtt
     6241 gcgatcgaac cagtcgctgt tgcccggcag atgggcgggc gccgcgacgc cgaccaggtc
     6301 gctgactcga tcccagttgg attcgatttc gaccggctcg gccgccgcct ttcgcacggg
     6361 cgtcttgtcg ggtttcaatt ccggggcgag gcgtcgcgcc tcctcgcgcg cggcctcgcg
     6421 caagggtcca tcgggcaatt gctgcgactg ggtcaaaagc cagcgcgcct ccgcccggtt
     6481 gagcttgcgc agtcgccccg gttgggcgcc ggcggactgt tccagctcgc gcgcctgctc
     6541 gaatcgctgc tggtcaagat agtattccgc cagctcccgc gcgatgcgtt ggccttcggc
     6601 gccataaggt tcctgcgcca gccgcagcca ggcggcctgc gcatcggcga cctcttcggc
     6661 cggcaccggc cgggcgaaca gggcgtaacc ggcccggccg atcacctcaa acacatagaa
     6721 caagccaacg ttttccatgg catcgagggc cgtcgtcatc cacgtagccg ccttgtaggt
     6781 tcgctcgcgc gcgtgttcgg gcccgacgaa aatgaaacgc cagacggccc cgcgccggcg
     6841 atcctcggcc cgttccaggc ggacgtccgg attgtgttgg gccgaagcgc gcagagcctc
     6901 cagccagcgg cgctcggccg gcggcgcctg cgcatgctcc tcgatcaggc gggcccacgg
     6961 gcgcatccgc gtcgactgac tctggcccgg tttgggcaac gccgccacgg ccgccgcggc
     7021 cagcgccgcc gttcccgtcg tcggttgtcc caaaagcagg gcgcgcagat gatcgggcgg
     7081 cgtcaggtca accggcggcg cgcggtcggg atagccgacc tgagccgatg cgaccgcctc
     7141 gcggcgcagc cgggccgcct ccgcctcggc cgtgcgcgcc agtgcagcgg cgccggacca
     7201 ctccggctgc aagccgctgg cctggcgcgc gtaaaacgcc gccacctcgg gccgacccgc
     7261 ctccagcgcc cattgcgcgc gttccatgtc ggcttcagcc agtgcccggt caaccgcctg
     7321 ttccaactcg cgcagcgcct cctgattcgc ggcgccgctc tggcgcaggg tttccgcctc
     7381 gcgcatcagg gcataagcgc gccggtcaag agcatcggcg gccgaggggc ggaaaaaact
     7441 gaagatcgcg tcgacgccaa gctgcagccc ggcctgcacg ttgccctgga cggcgcgcca
     7501 tccggaaaag accagtcggt tgaaccagtt ggcgtaaaac ccgtagcggc ggatgaattc
     7561 cgcctgccgg ctttggaaca agggattctc gctgatcgtg cgcaggatca ctccgcgcag
     7621 cagcggatca tcggtcgttt ccgacagctc gcgcagggcc tcttcctgcc gcgcgggttc
     7681 aagattcatg gtcgtcgcga tgtaatcgcg ccaggcttcc agctccggct gcacgggccg
     7741 cgccgtggga tcctggccgg ccagccagtc gcgcatgagc tcggcggcct gccggctgag
     7801 gggggcggtg ctcaccgtat cctggagcgc agccggcgcg gcccccagcg ggaacggcat
     7861 cgtgagaacg agggccaggg ccagggctgc gcgcctcatg agcccggctc cgtcgcggtg
     7921 ccgggccggt ccgcggcgcc gcgcaaggcg tcaatctccc ggctcagcga ttcgatccgc
     7981 tgcagggttt cgcggttagg gccgggcagt tgcggctccg gcaagggggg cgccgggggg
     8041 ttctccagcc gccgctgcgg cgcggccgtg atctccatga ccgttgtctc aaagacctgc
     8101 atccggtagc gcagcaccag cgccagcgga ttctgatgcg tccccggctc ggccatgatg
     8161 tccggcagga aaggaatcag acggtggggg aaccgcctcc gcacgcgggc catcccctgc
     8221 tgttgcaggt tctcgatcaa cgaccggtag ggagcgattc cgccgccgta ggcgctcgac
     8281 agcaccagca gatcaccctc cctgggggga ggattatcgc gcaaggagcc ctgcatcagc
     8341 gccgccgtcg ccggctccag atagacctcg cggtcgttca gccagaatct gagctttgct
     8401 ccttgaatat cggcgttgta aagctcgttg gtgtagacgc gcgcgtcggc gcgcagatct
     8461 tcctgtgcat tgatgtagag cgccgcctgt ttgaggtcca gaaacgggct gccctgccac
     8521 acccccacca gcagggccat cagcagcgag taacccaggg ccgcccctgc cacggcccag
     8581 tagaccgggc gccgcttgac gtgccggtcg agcgtggcga agccgtggcc ggcgaacagg
     8641 caaaccagcg gcaggagggg cagcagatag cgcgcctgaa acgacgagaa gaccgactgg
     8701 agcgcgagga tcgccagcat cagatagagc gccagccagc cgaaccagcg cagccgcgcc
     8761 gtctcccagc gcgtgcgcag cagcccgtaa agcaggaagc cggtgatgcc cagcgtcagg
     8821 aaaacgggca atgcccgcag gaactcctcg aactgcagcc aatagacacc gtagagcgtc
     8881 tggaagaagt ccaattgcga gccgcgttcg ccgatctggc gaaaatgatt ttccagcggg
     8941 ccgggaccct ggcgcagcag ccagagcggc accgcgaccc aggggagcag cccccaccag
     9001 gtccggcgtt ccgttccact cggattccgg tgacgcagaa cccggtccag atgggctgag
     9061 gccagggcca tcggcggcaa caggaggatt ccctgatagc gcgtcagcgt ggccagcgtg
     9121 ccgagcagcg agccgatcag cagccattcg aaggtccgcc gcggcgacgg cgcgccccgg
     9181 ggaggggagg ggaacagttc aggcagcgcc tgttcgtagg cgagcagcag cgcgcccagc
     9241 gccgcgctcc acagcgccgt cgccgtcatg tcggtcatga cttgaacgct ccagcgcagc
     9301 gccatcgggc tgacgagcca taccacggcg gcgtaaatcg cggcgcgccg cccgaacagc
     9361 cgcagggcca tggcgaacaa gaccggcacg accgcggccc cggccagcca ggagacaatg
     9421 cgcgccgaac cctccagacc gacgccggga agccagacga acaaccgcga caacaatccg
     9481 tagagcggcg gccagaacgt caggccctga cggaaggcgt cgatctgcag gatgccgtcg
     9541 gtgtaggccc cctgtgttac cggaaagaac aggccgcgcc agagcaatgc cgacaccgtc
     9601 agcaccacca gcagtttctc gttggcggtc aggcttctca tcgcgtcctc cgcagcccct
     9661 gcgcgggcgg cgtgatccgg tcgtcgtcgg ccgacaaggc gagtttgaac cgcggcgcgg
     9721 cccacttcaa gatcagcacg ccgagcaggc tggtcaggat caaaccgaac ggatgctccc
     9781 acggcatcgc cagcggcgcg tagacccaga acagccggcg caccagcccc gacacgaact
     9841 ggttcagcat caacaggccc agcagcagca gccagcgcga ggcgaaactg gtgaaccgcc
     9901 cgcggcgggc ggcctgaccc gccagatagc ccgtcaggct gagaccgacg atcatcgccc
     9961 ccagactgtt gttgggcggc tgggagaagc agaccagcag tgccgccacg acgcccatca
    10021 gcatgccggc gatctcgccg cgcagaaatc cgagcaacag cgccggcagc gccagcaacg
    10081 gccagtgata gagaaaatgc cagccgtgcg gactgaaccc ctgcagggtc ggcaggacga
    10141 tgacctcgat cggcagcgcg atgaacagat agagcagggt cagcaggagc aagcgcgtca
    10201 tgcgtcgggc gcctccggcg gctccagcgg cgagacgccc agccccaggg tggatggcgc
    10261 gtcgtcgtgc gccaggccgc ccgtcgcagg ctcgtcggcg cggagcgtgc tggttgccgg
    10321 cgcatcgaag cggaaaatga gcggctcggt ggtcgcctca tcgcgcggcg gcggcggcgg
    10381 gcccatcagc ggctgttccg agcgcggcgt gggcatgccg atccagtact gccccagcgt
    10441 cgcggtggtc gcgatcgccc cgcgcgccgg acgatgcgcc ggcacgacca gcagcacttc
    10501 ctccacggca tccagccgga cgctcggctc aaccaggcca tagggatatc cctcggcgtt
    10561 caccccccgc gcggtgatgc ggccgatttc aatccccttg ggatagaccg agttttcgaa
    10621 acccgacgtg atcagcattt cgccttccgg aatcggggtt gtttcgtttt cgggcatgaa
    10681 ttccagcggc gcctccagcc ccgaaccgcg caccacgccg cgcgagcgcg tctgcgccgt
    10741 caccgcgccg gcggcgaaca gcggatcgcg cacggtcagc acctgcgctc cgtcgcggtc
    10801 gaccatctgg acaatgccga ccagcccgcc gagcgccacg acgggatcac gcggggcaat
    10861 gccgtcctcc gcgccgcggt taatcatcag gaccgccccg cgatcgcgcg ccagcaggcc
    10921 ggtcacgtcg gccagcagaa attggagttg cgggggggcg gcccagcggc ccaggcccgt
    10981 cagccgttcc aatcgcccca cgcgttcgat caactgctgg ttttccagcc gcgcctggct
    11041 cagctcgctg cgcagccggc ggttctcctc ctcggcggcc cacagtgcgt cgatccgctg
    11101 gatctgtttg atcaccccgg tggtgcgcac ctggatcggc tccaggtagc gggccagcac
    11161 cccccgcacg cggccccaga cccggtaggt ctcatcgacg cggaagcgct ccagcgtgca
    11221 gaccgtcaac cccgccacga cgaacgcgcc cagcacgaac agacgcagac gagaacgcca
    11281 ggccaccgat ggcgaacgcg atgccgacga cacatgcagg tccttgagag cgaggatttc
    11341 gctggcggac gggccggcct cctatcatcg ggattgcccc ggagcatcgc cactcaaaat
    11401 tcgtcccgcg aaattcgatc tcacaaccct catcctcttc cttaccttaa agtggggcgc
    11461 atcgcgccgg ccgccggttt tccgccatgc gactgccgca atgacgtgct atgattcgcc
    11521 gcatgaaagg atcgcaggat ggacgaacga gaagacggtt ccgttccagc ggcccctgat
    11581 gccgagaatg aagtcggcgg cgcgcgcgac ggaccggtct atggtcgcgc gcgagccggc
    11641 tcgccggccg ttcccccgcc gggatgggat cctgagtcct cagccggatc ggccgctccg
    11701 gccgcggcgg aatcggccgt ctggacgtat cccggggcgg ggcaaacgcc gtggggcgaa
    11761 cccatcccgc cgcgcgacgg accggcctgg gaatggcgcc gcgaactggg caccgtccgc
    11821 gccgcctggc tgaccgtgcg cgagatcttc acttcgcccg accaggcctt ttcgcgcgcg
    11881 atccgcgacg gcggcctggg cgcaccgttt cagttctaca ttctgatggg ttacgtcttc
    11941 ggcattctgg gtttcgtgat ttcctggccg atccaattgc ttgccgaacg cgtgatgggc
    12001 gggatgttcc ctggaacgag cgggggcgtt gctgcggaat ggcccccttt catgcgcttt
    12061 cttgacaacg gctcgtcctt tggcgtggcg ctgctgatcc agatcgtgct gctgcccttc
    12121 acgttgacgg ccatcctgtt cgtcggcagc gcgatccttc acgtgttcct gaagctgctc
    12181 ggcggcggcc gcgcgggctt tgaggcctcc ttccgcgccg tggcttacgc ccagggttcc
    12241 gtcacgccat ttcaaatcgt gccgttcgtc ggaccgatgg tggcgatggt tctggggatc
    12301 atctttctga tcatcgggct ggcgcggatg cacgagaccg ccacctggcg cgcggtgctc
    12361 gcggtcctgc tcccgttcat gctgataacc gtgtttgcgg ttctcgccgc cgtcgttgtc
    12421 attctcgcgc tgtacggggg tgcgcggggg ttctgaaatc gtcgctcatc ctcgtgctcg
    12481 gggtcgacac gatccattct gccgtcccta cgggactctt tggattttgt ttccacggtt
    12541 tcccgccact gaagtagcgg gctgaagtct gccgtccctg acgggactgg ctcgctgaac
    12601 ggtccggcaa gctctcggct gctcgaagcg gtcgttaaag attctgctct tcaacccgcg
    12661 ttgtggaccc gttggttgag gatcggcgcc aacggaccca aagccgaatt gattcgaccc
    12721 tctcgtaaac tgccggttcg tgcgcaagcg tcatccttcc cgcaatcggt cgcgttccgg
    12781 cggattccac cgcacccctc ctcccgactc ccgtctcagg gttttcctga ggccgctcag
    12841 ggaatcaccg cgcaggggca gggattcgag accgtgaaaa ccgggatgat gctcatcggt
    12901 tcgcgacgcg cccgcgccga aacatgaact ggcggctgga aagggacttc ctggcggcca
    12961 cggcaaacgg acgagccaac ggtcgtgaag cgggataggg aatgattccc atgcacggaa
    13021 gctagacgct ccagggatgg agcccccgct tcagcgaccc gaccattgga atgaccacca
    13081 tgggccaaca gataccacag gcactgcagg aactgattga atcatcggtc tcgctgctgc
    13141 ccgaaaccac actggaaatc ctccggctgg cgccggatta ctcgaccacg aatctgacgc
    13201 tgcaacgcat gctggagcat gatcgccagc acgatctggc cgaccgggtc ctggcgttgg
    13261 cgaattcagt cgagtttcat gggctttcga cgatccggtc gatccccgac gccatcgcgc
    13321 gaatcggccg gctgcgattg ttcaacctcg tcctgacgag cgtcctgggc ggggtctacc
    13381 ggcgaaccga tgagtatgcg cggctgctgt ggcgccatgc gctcgcctgt ggatacgtct
    13441 gcgccggcct tgccgaacgg ttcaaagtgg ccgagtccga actcgcgttt gcggccggcg
    13501 tcctgcacga catcggcaaa ttcatcatct accggcgctt gcctcatctg tatcgccaat
    13561 tctggatcgg caaatcgcat gcgtttcggc tgattgagct ggaagcgcgg actttccctg
    13621 atttcgcgca cgcgcgagtg ggagccgccg cattgcgacg gatgggcctg ctgggcgttc
    13681 tggtcgaggc ggcggcgttg caccacgctt ttgaaagcgg gccggccaag gccagcaatc
    13741 cgggcctggt cagtgtcgtc tcgctgggca acgttctggt gaacaacgtc ggattggaac
    13801 gcaaggtttg cgagtggaaa gcgatcgagg agctctactg cgcgcgccac atcggcgcac
    13861 agggccggac gctggacgag gcgatcgccg cgctgccggg actgg
//
LOCUS       DAOPCO010000078        13776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4464_length_13776_cov_5.946578, whole genome shotgun sequence.
ACCESSION   DAOPCO010000078 DAOPCO010000000
VERSION     DAOPCO010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_13776_cov_5.946578"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..1711
                     /gene="tyrS"
                     /locus_tag="PLS90_07375"
     CDS             458..1711
                     /gene="tyrS"
                     /locus_tag="PLS90_07375"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPK02265.1"
                     /translation="MNETATVDIPEEIRAEWEKLTAGCVEVLPAEELRDRLVRAKRER
                     RPLRVKLGADPSAPDLHLGHTVALNKLRQFQELGHTVVFIVGDFTARIGDPTGRSETR
                     KPLTAEQIQANAETYLAQLFKILEPERTEVVRNSEWLDPMRFADVVRLTSQYTVARML
                     ERDDFEKRYRENRPIHIHEFLYPLVQGYDSIAVRADIEIGGTDQKFNLLVGRELMREA
                     GMAPQCIMTLPLLVGLDGVDKMSKSLGNYIGITEPPQEIYGKAMSVPDEMMRDYLVLT
                     LSYAPAAADELLGQTRDGRLHPRDLKARIAGELVARYHGAGAAREAAAHFDRVFRQRD
                     TPEEIEEASVAGEAEGLALVRALAQTGLAASNGEARRLITQGGVSVDGERVADANLVL
                     RPGAYLVKVGKRRFKKVVVTAGAER"
     gene            1815..2477
                     /locus_tag="PLS90_07380"
     CDS             1815..2477
                     /locus_tag="PLS90_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02266.1"
                     /translation="MASDRYAQAEWLELSGLPLELNRLRQGAWLVFKKLAELDCDANR
                     RPGEVEITVAELAARCGLEADKAARILELLQRKRRICCYLPEHPEEEGIFAIRSPVKT
                     PIPPERVAETTTNPFLRDLSNYRYARQEDEPPVDERKVQEAVDCYLNMLSTRMNVFIL
                     EQIEIAVRRFPLELVKQTIERAARHDIRSMKWVFKELIRDTARQEKKSPRDEARELGV
                     KL"
     gene            2558..3271
                     /locus_tag="PLS90_07385"
     CDS             2558..3271
                     /locus_tag="PLS90_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02267.1"
                     /translation="MKVIITGASAGIGAGIARVLAGEGFSLGLLARSGDKLRALCEEF
                     DRNQPDGYAVHASAACDLRDPQATETALHALIDRLDGVDALINNAGVVIRKDVWELTL
                     DEWRTLVETNLCGLFYATRAVLPTMREQRRGHLINVSSISGRMPLPGGSAYAATKYAV
                     AGFTESLFQEVRDFGIKATVLFPGSVDSASHRHDPAADHSWKVKPEEVGLACLDALNT
                     APGTCISRIEIRPLGRGPG"
     gene            complement(3286..4020)
                     /locus_tag="PLS90_07390"
     CDS             complement(3286..4020)
                     /locus_tag="PLS90_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02268.1"
                     /translation="MLEQFRPISAVLVLWPTPEFEFPEDHGAFAQRLRAVFPAFSEHR
                     LFQAAQGALPPELPRVALTGDRRRWTLELAPARITLARNLQGLTPAEAGLDTLLRELR
                     EQAGRVQAWLAENLNLRVYRIGLVVQFFCNTRSSANEKIAQYFLQPRALQGQTPHELN
                     LGLLNRLALADNVVVNRWLRVRPLRTNDARRLDFAAQIELDYNTLPEDTRQKTAREVA
                     EFIQAAERNMQDDIPVFNDVDFLGPR"
     gene            complement(4013..4807)
                     /gene="larB"
                     /locus_tag="PLS90_07395"
     CDS             complement(4013..4807)
                     /gene="larB"
                     /locus_tag="PLS90_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPK02269.1"
                     /translation="MNSDLLTQLLQQVCDRRIEIAEAVRRIKALPFEDLEFAKVDLHR
                     ALRHGRPEVIYCPGKTPDQVIQILRTLRRNGQHVLATRADPDLQAIVQAAVPEMRYHA
                     PARILTDLDLDDAEQGIGSVLVVAAGTSDLPVAEEAVLTARACGARVTPVYDVGVAGL
                     HRLLAQLDRIQQARAIVVVAGMEGALPSVVGGLCAAPVIAVPTSVGYGASFGGLAALL
                     GMLNSCASNVTVVNIDNGFGAGYVASLINQLPPPDPAPARRGDGVA"
     gene            5000..6301
                     /locus_tag="PLS90_07400"
     CDS             5000..6301
                     /locus_tag="PLS90_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK02270.1"
                     /translation="MSFPRSVISAAGCLLLVLTANGYAQKTGKTDPYFGLPYEKRQAL
                     KSYDMRNQPLDRPVVRAEPIPWTDAMGRLLVGAVMEEGKPRRTITKDELELRVNIYVK
                     TIDRSDLSSYDEEALFALRRQLLEDWAKTAALATYAERTGITVSEEEIDNALHELARN
                     HAPQGEALQNVGRMIGVPERELRQEVRDGLLVEKAVRREIRENVSIEEKRRIFQDQPN
                     AFLIPTHVDAWQIFAPQVGRLTESQREELMDELKDLRKELRRARHLDDVVRLRDKYGT
                     NEQFVITELNDIGEDEKLPRPVKEQLFGLDVGDTSEIIRSDLGWHIVKILDRREGHRG
                     SFEEALPQIEDYLFEISKDALYLSLQDHFDIPLNTHGLNQWRPVEAPRTAAADELTSS
                     GRPGVDAVRRLEDRRRRGGERIPSVEEVLSRRRQQTEREAF"
     gene            6301..6657
                     /locus_tag="PLS90_07405"
     CDS             6301..6657
                     /locus_tag="PLS90_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02271.1"
                     /translation="MSEDPRKPRWDDFKPVGETRAVPRRPLAGYVWLVIGLTVAGALA
                     GYCGTLGYDFWRGTPPRSGARHAAQRRAYERFMADRPTPEERAAYTRRAALFGAVGGL
                     VLGAGFCVKDLPRGGA"
     gene            6681..8813
                     /locus_tag="PLS90_07410"
     CDS             6681..8813
                     /locus_tag="PLS90_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02272.1"
                     /translation="MIKVLPILIGLLLVSPLRADRIELRDGRVIEGVLLDSPGANPAA
                     ESIRVRIGSSIITLPRANLQSVTPDSPGGNAVIRARDELQAGRITQALEFIEAAIAQG
                     PPGEALAALVLDHGMPLVIAAASLPPGDRARLSRLLQVLAAAAPDNAPLRIRRWQLHL
                     GLGEDAQAEALWSLLPVDLLQTDPGLREQVAGMLISQIDKASAAGDPERARAALEHLA
                     RIDAELADGRRIQFLLEQGWQRRRAEQYEEALELYSRELLPLSREIGRDRIAMTLQAA
                     ERALRSKDEFPRAAELYERYGLPVIPEEAREHIVDIWRDEGWRRMRREDFDGAAEAFA
                     RAEAVIPGAADADRLQLEFRRRLMLAVNESPAVRFELANWAYEQGLLEEAVALFHDLA
                     GDPRYGEAARAYENQLRNLLAEQKLSAMLDLYEAREYGQVLRDVGAFLGGDYPVGFKQ
                     QALEIQKLTRDAIAVLVAEEAQQAESMLQQAQRAFFREDYAEAEQLLDVIEERYRNTV
                     VYERAAEFRSLVGQKLALARLERGQQQTLAGGAQADASDAAGGADAGRAEQAPPPAAD
                     APADDTRMMESELDNLMRTLKRGNLAAQVQPATANRPAVSGTLPEYSPGELKARGLLD
                     QARQAYFDGRIGEADELLAEIRDDYAATPAAEEAARFYQLIRDQIELHERRAAAAASD
                     PPRPAAEPTTGTLPLAAPAGSEDFTQPR"
     gene            complement(8838..9686)
                     /locus_tag="PLS90_07415"
     CDS             complement(8838..9686)
                     /locus_tag="PLS90_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPK02273.1"
                     /translation="MLYVGTSGFSYDDWKGRWYPPALPRGRMFDFYAERFNAVEINST
                     FYSMAGPRMMASLVRRGAGRVVFAVKMPETVTHLGELTPELARDFARAMAPAAEAGLL
                     RAVLVQFPFRFRNTPGNRDYLERVVDAFADMPLVVEIRHASWQSEAGIDFFRRRRVSL
                     CLMDMPRLNGLPAASLELTGPIGYLRFHGRNRRQWWRAEYPGARYDYHYPDEELAAWL
                     EPIRRMRRQVENAFVFFNNHVNAQAPHNANTLLAMLGQPPPDPGYQDLFSSEPGPPDR
                     SDPTDS"
     gene            complement(9697..11127)
                     /locus_tag="PLS90_07420"
     CDS             complement(9697..11127)
                     /locus_tag="PLS90_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPK02274.1"
                     /translation="MAFSLDQFYRLNRRALIWIILFGLLWLLRGYFGLVFMTFVLAFI
                     ALPLARLLTTRFRLPRRLAITAVFAFILLAVVLFVVFVTPRVIAETNRLIGNLSQTEE
                     RLLELKDSLVSKYPSLDPILMGYIASSLEEDQLARIERDLEAAYPLPAPPPLVIQDDD
                     GGTTSVVVGTSLAVPGGLRDLPERGTLSPRDAAQLRQYNLAVAQREEIRGNMLVREFL
                     GSMRDRLRDQLPRLAIQLYRATITLLLALLFSFLITLDIARLGQEIRNLRHSKLHDFY
                     EEAAQPVVRFAFVVGRAIQAQAMIAMANTALTLVGLLLLGIPSLAMLSLIVFVCSFIP
                     VLGVFISTTPIVLVAINAGGLMPALAVIVLIIVIHMIEAYLLNPLIYGQHLKINPVLV
                     LIILFIGHHAFGLWGMLLGVPVASYFIHDVFGVPLWSESRLSSTGRAGPDVKEEELAA
                     RHETSPRLKSPPAAEPPGGGGPAGAS"
     gene            11410..12561
                     /locus_tag="PLS90_07425"
     CDS             11410..12561
                     /locus_tag="PLS90_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK02275.1"
                     /translation="MKSLLHRCALLLVVGGMLLSITGCRYATNRYYDFRDTFRVGVGA
                     TVENPVTGPLPPSFGLYLEATDWLNLGWITHNGYTAELDLRGTFVGPESMTRAGLLWW
                     QMLRINQDYDNAEYINPFKDRDFPWNHRMASPDYSFLGRPAKRLYYDHWANFMNKGTS
                     LLHRGYQYWEYTGVELAICEPFITHFGVMARLGVDLSEVFDFVLGFAYIDFKRDDLTP
                     EEYRLFRQWGDRSRDVTSVSDQTGRVDGEAVPESAPIEGPRPGDLLPLPEMKVIYFDY
                     DRSNIRSDQVPRMDFNLDYLRQNPDLHVMIEGHCDERGTIEYNFNLGLRRARAVRDYF
                     INAGIDPSRLQVVSKGEEEPAVLGTGESVWSLNRRAEFKRIVTIRAVRP"
     gene            12705..13058
                     /locus_tag="PLS90_07430"
     CDS             12705..13058
                     /locus_tag="PLS90_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPK02276.1"
                     /translation="MAGELTPFNPRRLEDLSGGDRDFERELIRTYRASARDALATLTQ
                     ALLSRNRAEIQQAAHKLKGMNSNLGAEGLAGLYQRVLDLLSQDSDFDAIEACVAEIEG
                     QQAALEDALHARANG"
     gene            13584..>13776
                     /locus_tag="PLS90_07435"
     CDS             13584..>13776
                     /locus_tag="PLS90_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPK02277.1"
                     /translation="MDTLQLKGLAFHGYHGTEPWEQEVGRRFVVDVTLHGDWTKAGRS
                     DDLNDAIDYRTIYATARKVL"
BASE COUNT         2393 a         4410 c         4896 g         2077 t
ORIGIN      
        1 cgaggcaaag ggaaatacaa tgttctgtcg ctgagtgcgc ccccgttcgg gggcgggatg
       61 gtggggcagc ggagattgcc ccgtgggggg gagttatgaa ttggggggat tgattgtgag
      121 ttgctgatga acctggggtt cacaacgagg gggccaaatg gcgagttctc gagagccgag
      181 gacgagggac gagagccgag agccgaatct tgggagggcc tttgagaggg atcacgagga
      241 cgggggacga atgggccgga agggggatgg ggcggggtgg ggggagatgc agggaccagg
      301 cagtcccgct cccgattttg atccccccgc ggtgcgcttg cgtcaacaat gcgcccaccg
      361 tgcgcttgtg gcgggcgggg ggaaacggta tgctggggcg actttagccg gcggcggggg
      421 gacccggacc ggcgcacacg gcgggggcgc aggcgctatg aacgagacgg cgacggtgga
      481 cattccggag gaaatccgcg cggagtggga gaagctgacg gcggggtgcg tcgaggtgct
      541 gccggcggag gagctgcgcg accggctggt gcgcgccaaa cgcgagcggc ggccgctgcg
      601 ggtcaagctc ggcgcggatc cctcggcccc cgatctgcac ctgggccaca cggtggcgct
      661 gaacaagctg cgccagtttc aggagctggg ccacacggtg gtcttcatcg tgggggattt
      721 cacggcgcgg atcggcgacc cgaccgggcg cagcgagacg cgcaagccgc tgacggccga
      781 gcagatccag gccaacgccg agacctacct ggcgcagttg ttcaagatcc tcgaaccgga
      841 gcggacggag gtcgtgcgca acagcgagtg gctggacccg atgcggttcg cggacgtggt
      901 gcggctgacg tcgcaataca ccgtggcgcg gatgctggag cgcgacgatt tcgagaagcg
      961 ctatcgggaa aaccggccga tccacatcca tgagttcctc tatccgctgg tgcagggcta
     1021 cgattcgatc gcggtgcggg cggatatcga gatcggcgga acggaccaga agttcaacct
     1081 gctggtgggg cgcgagctga tgcgcgaggc ggggatggcg ccgcagtgca tcatgacgct
     1141 gccgctgctg gtggggctgg acggcgtgga caagatgagc aagagcctgg gcaactacat
     1201 cggcatcacc gagccgccgc aggagattta cggcaaggcg atgagcgtgc ccgacgagat
     1261 gatgcgcgac tacctggtgc taacgctgtc gtatgcgccg gcggcggcgg acgagctgct
     1321 gggccagacg cgcgacgggc ggctgcatcc gcgcgacctg aaggcgcgga ttgccgggga
     1381 gctggtggcg cggtatcacg gcgcgggggc ggcgcgggaa gccgccgcac atttcgaccg
     1441 ggtgttccgg cagcgcgaca cgccggagga gatcgaggag gcgagcgtcg cgggcgaggc
     1501 ggaggggctg gcgctggtcc gggcgctcgc gcagacgggc ctggcggcgt ccaacggcga
     1561 ggcgcgccgg ctgatcacgc aggggggcgt ctcggtcgac ggcgagcggg tggccgacgc
     1621 caatctcgtg ctgcggccgg gggcgtatct ggtgaaggtg ggaaaacgac ggttcaagaa
     1681 agttgtcgtg acggccggcg ctgagcgttg agcgtgaacc cgcggggagg cagcggccgc
     1741 gggcaggcgc cggcgcgggc gcggactggc atgtcgtttg catcgcgagt gggttcgcgg
     1801 atgatgggaa gccgatggca tcggatcgct acgcccaggc cgaatggctc gaactgagcg
     1861 ggttgccgct ggaactgaac cggctgcggc agggggcatg gctggttttc aagaagctgg
     1921 ccgagctgga ctgcgacgcc aaccgccggc cgggggaagt cgagatcacc gttgcggagc
     1981 tggcggcgcg ctgcgggctg gaagccgaca aagccgcgcg gatcctggag ctgctgcagc
     2041 gcaagcggcg gatctgctgc tacctgccgg agcatccgga ggaggagggg atcttcgcga
     2101 ttcgttcgcc ggtcaagacg ccgataccgc ccgaacgcgt cgccgaaacg acgacgaacc
     2161 cgtttctgcg cgacctgtcg aactatcgct atgcgcggca ggaggacgaa ccgccggtcg
     2221 acgagcgcaa ggtgcaggag gcggtcgact gctacctgaa catgctcagc acgcggatga
     2281 acgtgttcat tctggagcag atcgagatcg ccgtgcgccg gttcccgctg gagctggtca
     2341 agcagacgat cgagcgggcg gcgcgccacg acatccgctc gatgaaatgg gtgttcaagg
     2401 agctgatccg cgacacggcg cgccaggaga agaagagccc gcgcgacgag gcccgcgagc
     2461 tgggcgtgaa gctgtagcgc gggaggaatg tggaaagcgg gcgaggacgg acgaggacag
     2521 acgaggatga gggacatttt ttgtgtgagg atcggtcatg aaagtgatca tcacgggggc
     2581 gagcgcgggg atcggcgccg gcattgcgcg ggtgctggcc ggcgagggct tttcgcttgg
     2641 gctgctggcc cgctcgggcg acaagctgag ggcgctgtgc gaggagtttg accgcaatca
     2701 gcccgacggc tacgcggtcc acgcgtcggc cgcgtgcgat ctgcgcgatc cacaagcgac
     2761 cgagaccgcc ctccacgcgc tgatcgaccg cctggacggc gtcgatgcgc tgatcaacaa
     2821 cgccggcgtc gtgatccgca aggacgtctg ggagctgacg ctggacgaat ggcggacgct
     2881 cgttgagacg aacctgtgcg ggctgtttta cgcgacgcgg gcggtgctgc cgacgatgcg
     2941 cgagcagcgc cgcggccatc tgatcaacgt ctcgtcgatt tcggggcgga tgccgctgcc
     3001 ggggggcagc gcctacgccg cgacgaaata cgccgttgcc ggcttcaccg agtcgctgtt
     3061 tcaggaggtt cgcgatttcg ggatcaaggc caccgtgctc tttccggggt cggtcgactc
     3121 cgcttcgcac cgccacgatc cggcggccga tcactcgtgg aaggtgaagc ccgaggaagt
     3181 cggcctggcc tgcctggacg cgttgaacac ggcgccgggg acgtgcatca gccgaatcga
     3241 aatccggccg ctgggccgcg gaccgggttg acccgccgct cgcgcctagc gcgggccgag
     3301 aaaatcgaca tcgttgaaca ccgggatgtc gtcctgcatg tttcgttcgg cggcctgaat
     3361 aaactcggcg acctcgcgcg cggtcttctg gcgcgtgtcc tcgggcagcg tgttgtagtc
     3421 cagctcgatc tgggcggcga aatcgaggcg gcgcgcatcg ttggtgcgca ggggccggac
     3481 gcgcagccag cggttcacga cgacgttgtc ggccagcgcc agccggttga ggaggcccag
     3541 gttgagctca tgcggcgtct gcccctgcag cgcgcgcggc tggaggaagt actgggcgat
     3601 cttctcgttg gcgctcgagc gcgtgttgca gaagaactgg acgacgaggc cgatgcggta
     3661 aacgcgcagg ttgaggtttt cggccagcca ggcctggacg cggccggcct gttcgcgcag
     3721 ctcgcgcagg agggtgtcca ggccggcttc ggccggcgtc aggccctgca ggttgcgcgc
     3781 gagcgtgatg cgggcggggg ccagttcgag cgtccagcgg cgccggtcgc cggtcagcgc
     3841 gacgcgcggc aactccggcg gcagggcccc ctgcgccgcc tgaaacagcc ggtgttcgct
     3901 gaaggcggga aagacggcgc gcaggcgctg ggcgaacgcg ccgtgatcct ccggaaactc
     3961 gaattcgggg gtcggccaga gcaccagcac ggcggagatc ggtcggaatt gctcaagcaa
     4021 cgccgtcacc tcggcgcgcc ggcgcggggt ccggcggcgg aagctgattg atcagcgagg
     4081 ccacgtaacc cgccccgaag ccattgtcga tattgacgac ggtgacattg gaggcgcagg
     4141 aattgagcat gcccagcagc gcggccagac cgccgaacga cgcgccgtag ccgaccgagg
     4201 tcggcacggc gatcaccggc gcggcgcaga gcccgccgac gacgctcggc agggccccct
     4261 ccatccccgc cacgacgacg atggcgcgcg cctgctggat cctgtccagt tgcgccagca
     4321 ggcggtgcag gccggccacg ccgacatcgt agaccggggt cacgcgggcc ccgcaggcgc
     4381 gcgccgtcag cacggcctcc tcggccaccg gcagatccga ggtgccggcg gcgacgacca
     4441 gcaccgagcc gatgccctgt tcggcgtcat ccagatccag gtcggtcaga atccgcgccg
     4501 gggcgtggta gcgcatttcg gggacggcgg cctggacgat cgcctgcagg tcggggtcgg
     4561 cgcgcgtcgc caggacgtgc tggccgttac ggcgcagcgt ccgcaggatc tggatcacct
     4621 gatcgggggt cttgccgggg caatagatca cttcggggcg gccgtggcgc agcgcccggt
     4681 gcagatcgac cttggcgaat tcaagatcct cgaacgggag cgccttgatg cggcggaccg
     4741 cctcggcgat ttcgatccgg cggtcgcaaa cctgttgcag aagctgagtc agcagatccg
     4801 aattcatgcc acgcttcaac ggggccgctc agatggcgga agtccctcgt agggcagtct
     4861 caagcccatg cccccccttt tcaagctgaa aatgagattg tcggggcaac ggacgattcg
     4921 gcagaatggt acgaaggcgc gccgcgatcc agccccgcgg gtcgggcgct cgccgctctg
     4981 tgcggaggtt ctgcccatca tgtcgttccc gcgttcggtg atctccgcgg ccggctgtct
     5041 gctgctcgtg ctcaccgcga acggctatgc ccagaagacg ggcaagaccg atccgtattt
     5101 cgggctgccc tacgagaagc gccaggcact caaatcgtac gacatgcgca atcagccgct
     5161 cgaccgtccg gtcgtgcggg ccgaaccgat cccgtggacc gatgcgatgg gccggctgct
     5221 ggtgggcgcc gtcatggagg agggcaaacc gcgccggacg atcaccaagg acgaactgga
     5281 actgcgcgtc aacatttatg tgaagacgat cgaccggtcc gacctgagct cctacgacga
     5341 ggaggcgctc ttcgccctgc gccgtcagct cctggaggat tgggccaaga cggccgcgct
     5401 cgccacctac gccgagcgga ccggcatcac cgtttccgag gaggaaatcg acaacgcgct
     5461 gcacgagctg gcgcgcaacc acgcgcccca gggcgaggcg ctccagaacg tcggccggat
     5521 gatcggggtt ccggaaaggg aattgcgcca ggaagtgcgc gacggcctgc tggtggaaaa
     5581 ggcggtgcgc cgcgaaatcc gcgaaaacgt gtccatcgag gagaaacgcc ggatattcca
     5641 ggatcaaccc aatgccttcc tgatccccac gcatgtcgat gcgtggcaga tcttcgcgcc
     5701 gcaggtcggg cggctgaccg aatcccagcg cgaggagctg atggatgagc tgaaagacct
     5761 gcgcaaggaa ctgcgccgcg cccgccatct ggatgatgtc gtgcggctgc gggataaata
     5821 tgggacgaac gaacagttcg tgatcaccga gctgaacgac atcggcgagg atgagaagct
     5881 gccgcgtccg gtcaaggagc agctcttcgg cctcgatgtc ggcgacacca gcgagatcat
     5941 ccgctccgat ctgggctggc atatcgtcaa gatcctggac cggcgcgagg gccaccgcgg
     6001 ttcgttcgag gaagcgctgc cccagatcga ggactacctg ttcgagatct ccaaggacgc
     6061 gctctatctg agcctgcagg atcatttcga catcccgctc aacacgcatg ggctcaacca
     6121 gtggcggccg gtggaggcgc cgcggacggc cgcggccgac gagctcactt catctggccg
     6181 ccccggcgtg gatgcggtcc gccggctgga ggatcgacgc cgccgcggcg gcgagcggat
     6241 cccctcggtc gaggaagtgc tctcccgccg gcggcagcag accgagcgtg aagcgttctg
     6301 atgagcgagg atccgcgcaa accgcgttgg gacgacttta agcccgtcgg ggaaacgcga
     6361 gccgttccac gccgaccgct ggccggctac gtctggctgg tcatcggcct gaccgtggcg
     6421 ggggccctgg ccggctactg cgggacgctg ggctatgatt tctggcgcgg gacgccgccg
     6481 cggagcggcg cgcgccacgc ggcccagcgc cgggcctacg agcgtttcat ggccgaccgg
     6541 cccacccccg aggaacgcgc cgcctacacc cgccgcgccg ccctgttcgg cgccgtgggc
     6601 ggcctggtgc tcggcgccgg tttctgcgtc aaggatctcc cgcgcggcgg cgcatgaccg
     6661 gagcctggat gattcgtccc gtgatcaaag tcctgccgat cctcatcggg ctgctgctgg
     6721 tatccccgct gcgcgccgac cggatcgagc tgcgcgacgg gcgggtgatc gaaggcgttc
     6781 tgctggattc gcccggggcg aacccggccg ccgaatccat ccgtgtgcgg atcgggtcga
     6841 gcatcatcac gctgccgcgc gcgaatctgc aatcggtcac gcccgactcg ccggggggca
     6901 acgccgtcat ccgggcgcgc gacgaactgc aggccggccg gatcacccag gcgctggagt
     6961 tcatcgaggc cgccatcgcg caagggccgc cgggcgaggc gctggccgcg ctggtgctgg
     7021 atcacgggat gccgctggtg atcgccgccg cgagcctccc gcccggcgat cgcgcgcgcc
     7081 tgtcgcgtct gctccaggtg ctggccgccg ccgcgcccga caatgcgccc ctgcgcatcc
     7141 ggcgctggca actgcatctg gggctgggcg aggatgccca ggccgaagcc ctctggagcc
     7201 tgctgccggt cgatctgctg caaaccgacc cggggctgcg cgaacaggtg gccgggatgc
     7261 tgatcagtca gatcgacaag gcttcggcgg cgggcgaccc ggagcgggcg cgcgcggcgc
     7321 tggagcatct ggcgcggatc gacgccgaac tggccgacgg ccgccgaatt caattcctgc
     7381 tcgagcaggg atggcagcgg cgcagggccg aacagtacga ggaggcgctg gaactttact
     7441 cacgcgagct gctgccgctg tcgcgcgaga tcgggcgcga tcggatcgcg atgacgctcc
     7501 aggccgccga gcgcgcgctg cgctcgaagg atgaatttcc ccgcgccgcc gagctgtatg
     7561 agcgttacgg cctgccggtc atccccgagg aggcgcgcga gcacattgtc gacatctggc
     7621 gcgacgaagg ctggcggcgc atgcggcgcg aggatttcga cggggccgcc gaggccttcg
     7681 cccgcgccga ggcggtcatc cccggcgcgg ccgacgccga ccggctccag ttggagttcc
     7741 gccgccgtct gatgctcgcc gtcaacgaaa gccccgccgt ccggttcgaa ctggccaact
     7801 gggcctacga acagggcctg ctggaagaag cggtggcgct gttccatgac ctggccggcg
     7861 acccgcgcta cggtgaagcc gcccgggcct acgaaaatca attgcgcaac ctgctggccg
     7921 aacagaagct ctcggccatg ctggatctgt atgaggcgcg cgaatacggg caggtgctgc
     7981 gcgacgtcgg cgcgttcctc gggggcgatt atcccgtcgg cttcaagcag caggcgctcg
     8041 agatccagaa gctgacgcgc gacgcgatcg ccgtgctcgt cgccgaggaa gcgcagcagg
     8101 ccgaatcgat gctgcagcag gcgcagcgcg ccttctttcg cgaggattac gccgaggccg
     8161 aacagttgct cgacgtgatc gaggagcgct accgcaacac cgtggtctac gagcgcgccg
     8221 ccgaattccg ctcgcttgtc ggccagaagc tcgcgctggc ccggctggag cgcggccagc
     8281 agcagaccct ggccggcggg gcgcaggccg atgcgtccga cgccgcgggc ggcgcggatg
     8341 cgggccgcgc ggagcaggcc ccgccgccgg cggcggacgc ccccgccgac gacacgcgga
     8401 tgatggaaag cgaactggac aacctgatgc gcacgctgaa gcggggcaac ctggcggcgc
     8461 aggtgcagcc ggccacggcg aaccgccccg ccgtcagcgg aaccctgccg gagtattcgc
     8521 cgggcgagtt gaaggcccgc ggcctgctcg accaggcgcg gcaagcctat ttcgacggcc
     8581 ggatcggcga ggcggatgaa ctgctcgccg agatccgcga cgactatgcg gcgaccccgg
     8641 cggcggagga ggcggcgcgc ttctaccagt tgattcgcga tcagattgaa ctgcacgagc
     8701 ggcgcgcggc ggcggctgca tccgatccgc cgcgcccggc cgccgagccg accacgggca
     8761 ccctccccct cgccgcaccg gcgggcagcg aggatttcac tcagccgcgc tgaccgaccg
     8821 acgggcacga tccctcttca agaatcggtt ggatccgacc gatccggcgg acctggttca
     8881 ctactgaaca ggtcctgata gccggggtcg ggcggcggct ggccgagcat ggcgagcagc
     8941 gtgttggcgt tgtgcggcgc ctgcgcgttg acatggttat tgaaaaagac aaaggcgttc
     9001 tcgacctggc gccgcatccg ccggatgggc tccagccacg ccgccagttc ctcgtccggg
     9061 taatgatagt catagcgggc gccggggtat tcggcgcgcc accattgccg ccgattgcgg
     9121 ccgtgaaaac gcaggtagcc gatcggcccg gtcagctcaa gcgacgcggc gggcaaaccg
     9181 ttcagccggg gcatgtccat caggcacaga ctgacccggc ggcggcggaa gaagtcgatt
     9241 ccggcctcgc tctgccaact ggcgtggcgg atctcgacga cgagcggcat gtcggcgaac
     9301 gcgtcgacga cccgctccag atagtcccga ttgccggggg tattgcggaa ccggaaggga
     9361 aactgaacca ggacggcgcg cagcaggccg gcctcggcgg ccggcgccat ggcccgcgcg
     9421 aaatcgcgcg cgagttccgg cgtcagctcg cccaggtgcg tcaccgtttc gggcatcttg
     9481 acggcgaaga cgacccggcc ggcgccgcgc cgcaccagcg acgccatcat ccgcgggccg
     9541 gccatgctgt aaaacgtcga gttgatttcg acggcgttga agcgctcggc gtagaagtcg
     9601 aacatccggc cgcgcggcag agcgggcgga taccaacgcc ccttccagtc gtcgtaggag
     9661 aagccgctgg tgccgacgta gagcatgggc ggcgcgtcag gaggcgccgg cgggtccccc
     9721 gccgccgggc ggttccgcgg cggggggcga tttcagccgc ggcgaggttt catggcgcgc
     9781 cgccagttcc tcttccttca cgtccggccc cgcccgccct gtcgaactca gccggctctc
     9841 cgaccacagg ggcaccccga acacgtcgtg gatgaaatac gacgccaccg gcacgcccag
     9901 cagcatcccc cacagcccga acgcgtgatg gccgatgaac aggatgatca gcaccagcac
     9961 ggggttgatc ttgagatgct gaccgtagat cagcgggttg agcagatagg cctcgatcat
    10021 gtggatcacg atgatcagca cgatgaccgc cagcgcgggc atcagcccgc cggcgttgat
    10081 cgccaccagg acgatcggcg tcgtcgagat gaacaccccc agcaccggga tgaagctgca
    10141 gacgaagacg atcagcgaga gcatcgccag cgacgggatg cccagcagga gcaggccgac
    10201 gagcgtcagc gcggtgttgg ccatggcgat catcgcctgg gcctggatcg cgcggccgac
    10261 gacaaaggcg aagcgcacga ccggctgcgc ggcttcttcg taaaaatcgt gcagcttcga
    10321 gtggcgcagg ttgcggatct cctggcccag ccgcgcgatg tcgagcgtga tcaggaagct
    10381 gaacaggagc gccagcagca gcgtgatcgt ggcgcggtag agctggatcg ccagccgggg
    10441 caactggtcg cgcaggcggt cgcgcatcga gccgaggaat tcgcgcacca gcatgttgcc
    10501 gcggatctcc tcgcgctgcg cgacggccag gttgtattgg cgcaactggg ccgcgtcgcg
    10561 gggactcagc gtgccgcgtt cgggcaggtc gcgcagcccc cccggcaccg ccagcgaggt
    10621 cccgacgacg acgctggtcg tgccgccgtc gtcgtcctgg atcaccagcg gcgggggagc
    10681 gggcagcggg taggccgcct ccaggtcgcg ttcgatccgc gcgagctgat cctcttccag
    10741 actcgacgcg atatagccca tcaggatcgg atcgagcgac ggatacttgc tgaccagcga
    10801 gtccttgagc tcgagcagcc gctcctcggt ctggctcagg ttgccgatca ggcggttggt
    10861 ctcggcgatg acgcgcggcg tgacgaacac gacgaacagg accaccgcca gcaggatgaa
    10921 ggcgaagacc gcggtgatgg ccaggcgccg cgggaggcgg aagcgcgtcg tcagcaggcg
    10981 cgccagcggc aaggcgatga acgccaggac gaacgtcatg aagaccagcc cgaaatagcc
    11041 gcgcagcaac cagagcaggc cgaacaggat gatccagatc agcgcgcgcc ggttgaggcg
    11101 gtaaaactgg tcgaggctga acgccatcga aatcgccctt gcgcggaatc ggcccggtcg
    11161 gcgcgaggcc gccgtcgcct cgtgtatagc aggcttggcc ggcgaaacgg aaggaaaacg
    11221 cgtgggccgg ccccggcgcc cggggaaatc gcgccccgcg ccggccggcc ccccgccccc
    11281 ttcggcgggg aatagaagtt gactttcccc cccggcaaag ctaacaagat catggtccta
    11341 tggggtccag gcctgccaat gggctcggga acaaccgcgc cgttttctaa ggatggagga
    11401 ttctccggca tgaagtccct gctacatcgt tgtgcgctgc tgctggtcgt cggcggaatg
    11461 ctgctgtcga tcaccggctg ccgatacgcg accaatcgct actatgattt ccgcgatacg
    11521 ttccgcgtcg gcgtcggcgc cacggtcgaa aaccccgtga ccgggcccct ccctcccagc
    11581 ttcggcctct atctggaagc caccgactgg ctcaacctgg gctggatcac tcacaacggc
    11641 tacaccgccg agctcgatct gcgcgggaca ttcgtcggcc ccgaatcgat gacccgcgcc
    11701 ggcctgctct ggtggcagat gctgcgcatc aaccaggact acgacaacgc cgaatacatc
    11761 aacccgttca aggaccgcga cttcccctgg aatcaccgga tggcgtcgcc cgattacagc
    11821 ttcctcggcc ggccggccaa gcggctctac tacgaccact gggccaactt catgaacaag
    11881 gggaccagcc tgctccaccg cggctaccag tactgggaat acaccggcgt ggaactggcg
    11941 atctgcgagc cgttcatcac ccacttcggc gtgatggccc ggctgggcgt cgacctctcc
    12001 gaggtcttcg attttgttct cggcttcgcc tatatcgact tcaagcgcga cgacctgacg
    12061 cccgaagaat accgcctgtt ccgccagtgg ggcgatcgct cgcgcgacgt cacgtccgtg
    12121 agcgaccaaa cggggagggt tgacggcgag gcggtccccg aaagcgcgcc gatcgaaggg
    12181 ccgcgccccg gcgacctgct tccgctgccc gagatgaagg ttatctactt cgactacgac
    12241 cggtccaaca tccgctccga tcaggtgccg cgcatggact tcaacctgga ttacctgcgc
    12301 cagaatcccg atctgcacgt gatgatcgag gggcactgcg acgagcgcgg gacgatcgag
    12361 tataacttca acctcggtct gcgccgcgcc cgcgcggttc gcgattactt catcaacgcc
    12421 ggcatcgacc cctcgcggtt gcaggtggtc agcaagggcg aggaggagcc ggccgtgctg
    12481 ggcaccggcg agagcgtgtg gtcgctcaac cggcgcgccg agttcaaacg gatcgtcacg
    12541 atccgcgccg ttcgcccctg accgggctgc ccggttttca tgacggcatc gccccggcgg
    12601 tctcggcgcc ccggggcgat tcttctaatg cgccgcgccc aggggccgcg ccggtatggg
    12661 gttgcccatt gagaaccggc gatcccacac tgaaagaaga tggcatggcg ggcgagctga
    12721 cccccttcaa cccccggcgc ctggaagacc tcagcggcgg cgaccgcgac ttcgaacgtg
    12781 agctgatccg gacctatcgc gccagcgccc gcgacgccct ggcgacgctg acccaggcgc
    12841 tgctcagccg caaccgggcg gagattcagc aagccgctca taaattgaag gggatgaaca
    12901 gcaatctcgg cgcggagggc ctcgcgggtc tctaccagcg cgtgctcgat ctgctcagtc
    12961 aggattcgga tttcgacgcc atcgaagcct gcgtcgccga gatcgagggg caacaggcgg
    13021 cgctagagga cgcgctgcac gcgcgcgcca acggctaggc ccccggcttg gagcggccca
    13081 cgctcggaaa tctgtctccg attcctccgg gattgcttcg atcgattctg ccgtccccac
    13141 gggactctgt tggcaggggt gacccggttc ccgccattga agtggcgggc tgaagtctgc
    13201 cgtccctgac gggactgccc tgctgaagga tccggcaagg cgcgggtcgt tcgaatcggc
    13261 cgtttgcccc cccattttca ttcctcgttg tgaacggccg gttcaaaagc aactcgtagg
    13321 aggattcgct tttcaccagg gggtataagg ggacaaaagg gacagaaggg acatagagga
    13381 cgcatgcagg ccattttcat ccgttcagga tgatgccgat ggcatcgtga aaaggtgagc
    13441 ctctttcgtg cccttgcgtg gctttggtga tccaactgtc cgaccgcttc gtgatcccat
    13501 cccgaaggtt atcgttgact ctcccatccc gacatgcaga ttagagtgcc gcaatctgtc
    13561 gtcctgtacc ggagaccaga cggatggata ccctgcaact taaaggcctc gccttccatg
    13621 gctatcatgg aacggagccg tgggaacagg aggtcggacg ccggttcgtc gtcgatgtca
    13681 cgctgcacgg cgactggacc aaagcgggcc ggagcgacga cctaaacgac gcgatcgact
    13741 accgcacgat ttacgcgacc gcccgcaagg tgctgg
//
LOCUS       DAOPCO010000079        13678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4497_length_13678_cov_5.787712, whole genome shotgun sequence.
ACCESSION   DAOPCO010000079 DAOPCO010000000
VERSION     DAOPCO010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13678
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_13678_cov_5.787712"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="PLS90_07440"
     CDS             <1..1205
                     /locus_tag="PLS90_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK02278.1"
                     /translation="LAVAHQALVPVRAELAIVQLLQDQVRNAAGTLDQIGKEFMTGDV
                     RQLRAIVDLYEGEPRAAVERLGEVDPASASVVVKALAQAAAGNAGAARESLAGQQVLT
                     RDATELMRRMVTERTAEELSRVAPVLALTIHLGQQPFFHKVALALTEDALGMLPDDPF
                     ILSRQGEIHLAMGDVAQARAVYERLWRAAPDFTPALTAQVETLQMQARQATARKNADE
                     AQSLLRHSRELLEQHLAEKPEDAVALEKLALVQQEQDELEESVETYRQVIRLDQDNWT
                     AYNNLAWNLARLGQLEEAARMGGEALKRQPENGGILDTVGWIELQRGNLERATELLRR
                     AARQIPNNAEIRFHLAQALARQDRKEEAARLLELILIAAPGYEKAGEVRSLLAEVAPE
                     AGAARGTN"
     gene            1358..2179
                     /locus_tag="PLS90_07445"
     CDS             1358..2179
                     /locus_tag="PLS90_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK02279.1"
                     /translation="MTLPVLAGGGIPLRGSLVVLGLALMLPAGCYRAKVLPTVDEEYA
                     IPLSEPRLVDHADVPLDEDPTQTYHVGLRDKLKIDVRKDPDLSGSYEVNLEGEIFLPL
                     IGTAKVEGLTTEEIEELLTEKLSQYIREPDVHVGMEEYNSKLIYVIGQVRQPGPLRME
                     ADALTLRQAVFGAGLPTPDAALKRCHVISPDYEDPVVYQIDLSEVLYQGKMRENILLR
                     PDDIVYLPAKYATNISNTIRELLRPIRDVEDLQRNRFLGDEDYYDSDRYRDRYRY"
     gene            2204..3790
                     /locus_tag="PLS90_07450"
     CDS             2204..3790
                     /locus_tag="PLS90_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPK02280.1"
                     /translation="MSRPSFDLSRMLLVLRTRKRYWIPLTLGAILAVNLLTLVMPKKY
                     ESVAKVRVYEPRVQVITTQAKDMLTVDDRLETISEEITSYNFLEQIAAKSTVNLTGAL
                     NPNSREYEKVVTRLSKDVKVYTGDELFEIHYKAPTPNQAYHVVNEILEEYLARTSEFY
                     GSRTRRIVELTEQQLAEAMREREKIQERIKEYKEEHPNEIPEAAQAHMAQLANLRSNR
                     QLAIQALEGERNAVQLALAKLKNTPEETVSKTTVEKSREVLQIRSEIRMLEMQVELKL
                     KDFREEHPSVVALRDQIAVLERQLEGLEAAPIEQVETSPNPEFQTLKEEIMRSELEIQ
                     KLQTQIAQQDREIARLESYMKQLPVNELELSKLESELQNADDLVKEYSESLSEARRIA
                     EVDQSGQGPSFEVIDRPRQSNKPVSPNPVKYGALSLVLGFGAGGLFIYLVALLDSAVR
                     TMEEARQVLRMPILGVVQTIQHPSGPLYPAPRRKFAPMRKAATFLVLLIGVGWAMLAV
                     SLFVSMAPGDDPVSRLFGGM"
     gene            3800..6133
                     /locus_tag="PLS90_07455"
     CDS             3800..6133
                     /locus_tag="PLS90_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK02281.1"
                     /translation="MTGNLDRVILELEGVLGEIIPDRFRERLLLIVERYRSERLGMVI
                     TTRIDRRRAEADRERGFFTPAGGGRPPVFTWKATGSLPAILRKFRVDFIGRLGEAELR
                     SRVYLALYLLEAKKPVQPPAAAAEPARPVVKPSAKPTVAAGAERRSRVLRREEPKAVE
                     SQSAPTSAPVVKAEKKAEAQAAGESAPARKPASAEVSRATTRMASSAPRAEATTGGSG
                     VGATSRRSAERPTAPKAETRAREARAGAAPARHERPPRGQSIMPAKRSQAEALQVEQA
                     EPVAEPPMPPQAARIIDVSVQALPAEEPAPAPTKRTAPAIEQEEMALDGPAVMPIEAP
                     RPKRSERSARREASAQKPARQPASEHGAAEPRRESQDTEPLRTAGIEGGEAPATAPIE
                     APDGVIESEEKPNSVVPAPTPVGRPAAAEPSVTSPAPQMPALQTPATQPAAPQPAAPQ
                     LAAASSTSAPSAPPPPAAQPAPRPAPTPGQIVRAPAPGGRALKLPGNFYQEFFGFDYM
                     PFNNTPDSRFYFPTEKHQEALSRMIYVISERKGFVMISGEIGSGKSTLCRTLLARLPR
                     EVKTALITHTHLDSTQIVHAIAEDLGLKPEGKDRFDTIRLINEYLIEQHRKGCTVCII
                     IDEAQNLTSETLEEIRMISNLETEEEKLVQLLLLGQPELRDKMRRPEMQQLRERIAVQ
                     FHLEPLTLKETIQYIQHRLRLATPRKGLEFPRSALEEVYRFSGGVPRLINGICDNVLL
                     TLYTRQLRKATAGIIREAARDMNLEPRVGAIKRIFGR"
     gene            6311..7219
                     /locus_tag="PLS90_07460"
     CDS             6311..7219
                     /locus_tag="PLS90_07460"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HPK02282.1"
                     /translation="MAKVLDALEQARRERLRKLRAAGARVEAPLNPTEPGDDLELELV
                     EAPQRESALRPGQRPVVAPAAHPHIAPEIVALHASDSPVAEQVRQIRTNLETVLAKLT
                     ARTIVITSPVSGDGKTLVAANLATILADDPRTRVMLVDADLRKPDQHRLFGVKGSPGL
                     AEYLRGRHELADVAVGTSIPNLDFIPAGFPPDKPTVLLSGERMGELLKTLEETYQWVL
                     FDTPPMLPVTDASLLARHVTGLILVVRVGSTTRGAIERTQEMLAETHLPILGCILNDF
                     NDHAAPGGYYHKAYGRAEAVAADEQG"
     gene            7216..8391
                     /locus_tag="PLS90_07465"
     CDS             7216..8391
                     /locus_tag="PLS90_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02283.1"
                     /translation="MSGAAAAEALFWAGALFILYTWFGYPLLMAGWARARPRPVRRGR
                     GHQPTMTAVVAARNEAAGIAARARNLLEQDYPTELLDIVLVSDGSTDATAEIIQALAA
                     AEPRVRAIVLPENVGKAAALNAAVEQARGEVLLFADARQRFERNVAAMLAENFADPAV
                     GSATGELMIERGGGVAEQVGLYWRYEKWIRRNEAASGSMLGATGAIYAMRRELWRPLE
                     RGALLDDFLAPMRAVLAGARAVFDERAIAWDRPSERSEQEFRRKLRTLAGNYQAFALE
                     PGLLLPWRNPTWLRVWSHKVFRLLAPYGLVLVFGGALFAPGGFYGALAVLQGVFYGLG
                     LAGIAAERRGRPVRSRVVRLAATFIVLHAAAVGGLWVWLRGGSAAGLWRKAYQENEG"
     gene            complement(8395..>11254)
                     /locus_tag="PLS90_07470"
     CDS             complement(8395..>11254)
                     /locus_tag="PLS90_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK02284.1"
                     /translation="ITTFPADAERPIITEITTRSPVLFIAIHGQLPERQLKELAEEVK
                     DELMLRDEITQVSVTGAREYEISIELSDEKLRRYGLTFDQVSRIVREASQNWPGGSLR
                     GEAEQLTLRTMARRYTGQEFGDIILLTQPDGASIRLRQVAEIHDSFVEDPIYARFNGE
                     PAVLINVSKTNEEDALKIREQVQEYIDHKLRQLPAGVGMHVWFDSSIFITQRIDMLVG
                     NGISGLIIVFLLLWLFLDLRLAIWCSLGIPVALAGALAIMGLTGQTINMISLFAMIMI
                     LGIIVDDAIVISESIYHRRRQGVGPADAAVQGAGEVAWPVFAAVTTTIVAFLPLFFIK
                     GVMGKFIRVLPLVVCSALLVSLLEALMMLPSHLRDLRPFRPLDETATGLRRAALRLRH
                     AFGAGLDRLIAGLYVPLMRHVLNWRYVTVAAAIAFLFLCLGVQRAGLVKFELLPDIDT
                     DYLIAQVEFPSGTPLSITRQAVERMEQALASVARELPTASGEPMVEALFSISGASTGF
                     NAQFGTHIGSLYVELLPTEQRGIFFQQIISRWEQAVGPIPGAVSVRYDTLDTGPGGKP
                     IEVWLMGHDMDHLLAAADRLKARLNQFDGVYQVEDDYRPGKREVRARLKPEARNLGLT
                     EEMLGRQLRQGFYGDEALRVQRGRDELRVWIRYPRDQRRSLGDIESIRIRTPDGREVP
                     LAAVADLDVEQGYAAINRRNGMRRIAVTAEVDTARANAHEIVDFLESGYLADLMRDFP
                     GLVASIEGQKRETQESLESLFIGFPLALLAIFLILATIFRSYAQPLVILVTIPFGLIG
                     AVIGHMVMGYHLTMLSMFGMVALAGIVVNDAIVLIDNVNERIARGVPVFSALCEAGAR
                     RFRAIMLTSVTTSFGLAPLILEKSMQAQFLIPMAISISFGVAFATNLTLILIPCLLLI
                     LNDLRRARVWLFRGRLPQREEVEPAAARRIQSEADAQI"
     assembly_gap    11255..11264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11265..11611)
                     /locus_tag="PLS90_07475"
     CDS             complement(<11265..11611)
                     /locus_tag="PLS90_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK02285.1"
                     /translation="MKQLSAWGVANPVFVNILFWIVIISGGVAAFTMVREVMPEFSVD
                     IIQVTVPYPGAGPEEVEEGIALKLEDAIEGLEGIKNFHTTATEFVGTATIEVLEGYDV
                     QKLKDEITDRVNA"
     gene            complement(11608..13023)
                     /locus_tag="PLS90_07480"
     CDS             complement(11608..13023)
                     /locus_tag="PLS90_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK02286.1"
                     /translation="MRIRSVIVRAVLVVLLIGGGAVVMNRLAALRKPPPQAETVERRL
                     RVEALRVDPLTLRTLLTGFGQARADRRVELIPQVAGRIIEVHPDLDVGRYIDRGETLF
                     RIDPRDYEAAVEQARAALDSLGAQKARLAQARENDQRRIEIARRTLELARRDFERFRQ
                     LVEVDRVESPARMDQAETAMNQAREQVVALENALALYPAQLRDLDAQLQSARARLEQA
                     RLDLERTRVTAPFTGRVDRKSIEIGQLVGPSMANAVTLVDDASLEIPVPLEGGEVARW
                     LDVQSLPDQPTWFSRLEGRPVVIRWTEQPDRVQWAGRLDRIERFDPQTRTVHVVARVT
                     RPLDGQSPAPSDFPLVEGMFCQVEIPGREVAGVYRLPRSAVGPDGNVLIVNDSRIHSR
                     AVHIARYEGDTVLIDDGLQPGQIVLTTQPGIVLDGTLVDVDLVDPQTLWDDAAASPGA
                     PVPAPPIERDERPRELEVTAP"
     gene            complement(13030..13518)
                     /locus_tag="PLS90_07485"
     CDS             complement(13030..13518)
                     /locus_tag="PLS90_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPK02287.1"
                     /translation="MTAKHDSPTAGTIYRNLLRVMAFLHRDLDHAKAWKDLGHDVSFP
                     QFRTMMVLRQIGPCSLKELADALEITAASTSEMVERLVDLGLVERRPDRNDRRRIQLS
                     LTPRAVKGVQRHEGLILKRIDALCERLGPGPANHWRRACAALDDLFDQQPSGGSTAAR
                     PR"
BASE COUNT         2356 a         3872 c         5380 g         2060 t
ORIGIN      
        1 agctggcggt ggcgcatcag gcgctggtgc cggtgcgggc ggaactggcg atcgtgcagc
       61 tcctgcagga ccaggtgcgc aacgcggcgg ggacgctcga ccagatcggc aaggaattca
      121 tgacggggga tgtgcgtcaa ctgcgggcga tcgtggacct gtatgaaggc gagccgcggg
      181 ccgcggtgga acggttgggg gaggtggacc cggcctcggc gtcggtggta gtcaaggcgc
      241 tggcgcaggc ggcggcgggc aacgccggcg cggcgcgcga gagcctggcc ggccagcagg
      301 tgctgacgcg cgacgcgacg gagctgatgc gacggatggt gaccgagcgg acggcggagg
      361 aactgagccg ggtggcgccg gtgctggcgc tgacaattca tctcggccag caaccgttct
      421 tccacaaggt cgcgctggcg ttgaccgagg atgcgctggg gatgttgccg gacgatccgt
      481 tcatcctgag ccggcagggg gagattcatc tggcgatggg cgacgtggct caggcgcggg
      541 cggtctacga gcggctctgg cgggcggcgc ccgacttcac gccggcgttg acggcgcagg
      601 tcgagacgct gcagatgcag gcacggcagg cgacggcacg gaagaatgcg gacgaggcgc
      661 agagcctgct gcggcactcg cgggagttgc tggagcagca cctggcggag aagccggaag
      721 atgcggtggc gctggagaag ctggcgctgg tgcagcagga gcaagacgaa ttggaagagt
      781 cggttgaaac ctatcgtcaa gtgatccggc ttgatcagga taactggacg gcctacaata
      841 atctggcttg gaacctggcg cggttgggcc aactggaaga ggccgcccgg atgggggggg
      901 aggcgctgaa gcgccagccg gagaacgggg ggattctgga cacggttggc tggatcgagt
      961 tgcagcgggg gaatctggag cgggcgacgg agctgttgcg ccgcgccgcg cgccagatcc
     1021 cgaacaatgc ggaaatccgg ttccacctgg cgcaggcgct ggcgcgtcag gatcggaagg
     1081 aagaggcggc gcggttgctg gaactgatcc tgatcgcggc gccggggtat gagaaggcgg
     1141 gggaggtccg ctcgctgctg gcggaggtgg cgcccgaggc gggcgcggcg cgcgggacca
     1201 actgagtggc cgcgcgggaa ggaagcgtga tacaaaggga aagcgaaacg acggctgctg
     1261 acaggagtcc cggagaagct gttgagcaca cgaggccggc gactggaagc ggaggtctcg
     1321 tgaagaagcg gtgcccgtag aagaaggaga acgtccgatg acgctgcccg tccttgccgg
     1381 cggggggatc cccctccgcg gaagtctggt tgtgctgggt ttggcgctga tgctgcccgc
     1441 ggggtgctat cgggccaaag tgctgccgac ggtcgatgag gagtatgcga taccgttgag
     1501 cgagccgcgg ctggtcgacc acgccgacgt gccgctggat gaggatccga cccagacgta
     1561 tcacgtcggg ctgcgcgaca agctgaagat cgacgtgcgc aaggatccgg atctgtcggg
     1621 cagctatgag gtgaacctgg aaggggaaat cttcctgccg ctaatcggga cggcgaaggt
     1681 ggaggggctg acgacggaag agatcgagga gctgctgacc gagaagctgt cgcagtacat
     1741 ccgcgagccg gacgttcatg tgggaatgga ggaatacaac agcaagctga tatacgtgat
     1801 cggtcaggtg cgccagcccg gcccgctgcg gatggaggcc gacgcgctga cgctgcgcca
     1861 ggcggtgttc ggcgcggggc tgccgacgcc ggacgcggcg ctgaagcggt gccacgtgat
     1921 ctcgcccgac tacgaggatc cggtagtgta tcagattgac ctgagcgagg tgttgtatca
     1981 gggcaagatg cgcgagaaca tcctgctgcg gcccgacgac atcgtgtatc tgccggcgaa
     2041 gtatgcgacg aacatcagca acacgatccg ggaattgctg cggccgatcc gcgacgtgga
     2101 agacctgcag cgcaaccgtt ttctgggcga tgaggactac tacgactccg accgctaccg
     2161 cgatcgctac cggtattaac agcgagaaca aaggctgaac gcgatgtcac gaccgagctt
     2221 cgacctgagc cgcatgctgc tggtcctgcg cacgcgcaag cgttactgga tcccgttgac
     2281 gctgggcgcg attctggcgg tgaacctgct gacgctggtg atgccgaaga agtacgagtc
     2341 ggtggcgaag gtgcgcgtct atgagccgcg ggtgcaggtc atcaccacgc aggccaagga
     2401 catgctgacg gtggacgacc ggctcgagac aatctcggag gaaatcacga gctacaactt
     2461 tctggagcag atcgcggcca aatcgacggt caacctgacg ggggcgctca accccaacag
     2521 ccgggagtat gagaaggtgg tgacccggtt gagcaaggac gtgaaggtct acaccgggga
     2581 cgagctgttc gagatccact acaaagcgcc cacgcccaac caggcgtatc atgtggtcaa
     2641 cgagatcctg gaggagtatc tggcgcggac ctcggagttc tacggcagcc ggacgcggcg
     2701 gatcgtcgag ctgaccgagc agcagttggc cgaggcgatg cgcgagcgcg agaagatcca
     2761 ggaacggatc aaggaataca aggaagagca tcccaacgag atcccggaag cggcgcaggc
     2821 gcacatggcg caattggcga atctgcgcag caaccgtcag ttggcgatcc aggcgctcga
     2881 gggggagcgc aacgcggtgc agctcgcgct ggcgaagctg aagaacacgc ccgaggagac
     2941 ggtctccaag acgacggtcg agaagagccg cgaggtgctg cagatccggt cggagatccg
     3001 gatgctggag atgcaggttg agttgaaact gaaggatttc cgggaggagc acccctcggt
     3061 cgtggcgctg cgcgatcaga tcgcggtgct ggagcgccag ttggagggtc tggaggcggc
     3121 gccgatcgaa caggtggaga cgtcgcccaa cccggagttc cagacgctca aggaagagat
     3181 catgcgctcg gagctggaga tccagaagct gcagacgcag atcgcgcagc aggatcggga
     3241 gatcgcgcgc ctggaatcgt acatgaagca attgccggtc aacgagctgg agctgtcgaa
     3301 gctggagagc gagttgcaga atgcggacga cctggtcaag gagtattccg agagcctgag
     3361 cgaggcgcgg cggatcgcgg aagtggacca gtcggggcag gggccgtcgt tcgaggtgat
     3421 tgatcggccg cggcagtcca acaaaccggt ctcgcccaat ccggtcaagt atggcgcgct
     3481 gagcctggtg ctggggttcg gggcgggcgg gctgttcatc tacctggtgg cactgctcga
     3541 ctcggcggtg cggacgatgg aagaggcgcg ccaggtgctg cgcatgccga tcctgggggt
     3601 ggtgcagacg atccagcacc ccagcgggcc gctatatccc gcgccgcggc ggaaattcgc
     3661 gccgatgcgc aaggcggcga cgttcctggt gctgctgatc ggcgtcgggt gggcgatgct
     3721 ggcggtttcc ctgtttgtct cgatggcgcc cggcgatgac ccggtgagcc gcctgttcgg
     3781 tggaatgtga gagcaggcaa tgacggggaa ccttgatcgc gtcatactcg agctggaagg
     3841 ggtgttgggc gagatcatcc cggaccggtt tcgcgagcgg ttgctgctga tcgtggagcg
     3901 ctaccgcagc gagcggttgg ggatggtcat cacgacgcgc atcgaccggc ggcgggcgga
     3961 agcggatcgc gagcgcggct tcttcacgcc ggcggggggc ggccgtccgc cggtgttcac
     4021 gtggaaggcg acgggctcgc tgccggcaat cctgcgcaag tttcgcgttg atttcattgg
     4081 acggctgggc gaggcggagc tccgctcgcg ggtgtatctg gcgctgtatc tgctggaggc
     4141 gaagaagccg gtccagccac cggcggcggc ggccgagccg gccaggccgg ttgtcaaacc
     4201 gagcgccaag ccgacggtcg cggcgggcgc ggagcgaagg tcgcgcgtgc tgcggcgcga
     4261 agagccgaag gcggtggagt cccaatcggc gccgacgtcc gcgcccgtgg tgaaggccga
     4321 gaagaaggcg gaggcgcaag ccgcggggga atccgcgccg gcacggaagc cggcgagcgc
     4381 cgaggtcagc cgcgccacaa cgcggatggc gtcgagcgcg ccgcgtgccg aggcgacgac
     4441 gggcggctcc ggcgtcggcg caacgagcag gagatcggcg gagcggccga cggcgcccaa
     4501 agcagaaacc cgcgcgcgcg aggcgcgggc tggggcggcc ccggcccggc atgagcgacc
     4561 cccgcgcggc cagtccatca tgccggccaa gcgatcgcag gccgaggcgc tgcaagtgga
     4621 gcaggcggag ccggtcgccg agccgccgat gccgccgcag gccgcgcgga tcatcgatgt
     4681 ttcggtgcaa gcgctgccgg ccgaagagcc ggcgcccgcg ccgacgaagc gaacggcgcc
     4741 ggcgatcgaa caggaagaaa tggcgctgga tgggccggcg gtgatgccga tcgaagcgcc
     4801 gcggccgaag cggagcgagc ggtccgccag gcgggaggct tccgcgcaga agcccgcccg
     4861 gcaaccggcg agcgagcacg gcgcggccga gcccaggcgt gaatcgcaag atacggaacc
     4921 tttacgaacc gcggggatcg aagggggcga ggcgcccgcg acggcgccaa ttgaggcgcc
     4981 ggacggggtg attgaatctg aggagaagcc gaattcggtc gttcctgctc cgacgcccgt
     5041 ggggcggccg gccgccgccg agccgagcgt aacgagtccc gctccgcaaa tgcccgcact
     5101 gcaaacgccc gcgacgcagc cggcggcgcc ccagccggcc gcaccgcagc tggccgcggc
     5161 gagttcgact tcggcgccca gcgcgccgcc gccgccggcc gcgcaaccgg cgccgcgacc
     5221 cgcgcccacg ccgggacaga tcgtgcgcgc gcccgcgccg gggggccggg cgctgaagtt
     5281 gccggggaat ttctaccagg agttcttcgg gttcgattac atgccgttca acaacacgcc
     5341 cgactcgcgg ttctacttcc cgactgagaa gcaccaggaa gcgctgtcgc ggatgatcta
     5401 tgtgatctcg gagcgcaagg gcttcgtgat gatctccggt gagatcggct cgggcaagtc
     5461 gacgctctgc cggacgctgc tggcgcggct gccgcgcgag gtgaagacgg cgctgatcac
     5521 gcacacgcat ctggactcga cgcagatcgt ccacgcgatc gccgaggatc tggggctgaa
     5581 gcccgagggg aaggaccggt tcgacacgat ccggctgatc aacgaatacc tgatcgagca
     5641 gcaccgcaag gggtgcacgg tgtgcatcat catcgatgag gcgcagaacc tgacgtccga
     5701 gacgctcgaa gagatccgga tgatcagcaa cctggagacc gaggaggaga agctggtgca
     5761 gctgctgctg ctggggcagc cggaattgcg cgacaagatg cggcggccgg agatgcagca
     5821 actgcgcgag cggatcgcgg tgcagtttca tttggagccg ctgacgctga aggagacgat
     5881 ccagtatatt cagcaccggc tgcggctggc gacgccgcgc aaggggctgg agtttccgcg
     5941 ctcggcgctg gaggaagtct accgcttctc ggggggggtg ccgcggctga tcaacgggat
     6001 ctgcgataac gtgctgctga cgctttacac gcggcaactg cgcaaggcga cggcggggat
     6061 catccgcgag gcggcgcgcg acatgaatct ggagccgcgt gtcggggcga tcaaacggat
     6121 ttttgggcgg tgagggtgcg tcgcgatgga tgaggccggc aaggatgccg gcggtcccag
     6181 gcggtggttg atccggcgga tgggttgatt ccgagagccg aggacgaggg gacgagagcc
     6241 gaatttcagg gcgggccgtt gggggcgatt acgaggacga gggacgattt tagcgtaagg
     6301 gaccgagacg atggcgaaag tactggatgc actggaacag gcgcggcgcg agcggctgcg
     6361 taagctgcgg gcggcgggtg cgcgcgtcga ggccccgctc aacccgaccg agccgggcga
     6421 cgacctggaa ctggaactgg tggaggcgcc gcagcgtgaa tcggcgttgc ggccgggtca
     6481 gcggccggtg gtggcgccgg cggcgcatcc gcacatcgcg ccggagatcg tggcgctgca
     6541 cgcctcggac tcgccggtgg cggagcaggt gcgccagatc cggacgaacc tggagacggt
     6601 gctggcgaag ctgacggcgc ggacgatcgt gatcacgagc ccggtttcgg gcgacggcaa
     6661 gacgctggtg gcggcgaacc tggcgacgat cctggccgac gatccgcgga cgcgcgtgat
     6721 gctggtcgac gccgacctgc gcaaaccgga tcagcaccgg ctgttcggcg tgaaagggtc
     6781 gccggggctg gccgagtatc tgcgcggccg ccacgaactg gccgacgtgg cggtggggac
     6841 gtcgatcccg aacctggact tcatcccggc cggatttccg ccggacaagc cgacggtgct
     6901 gctctcgggc gagcggatgg gcgaactgct caagacgctg gaggagacct accagtgggt
     6961 gctgttcgac acgccgccga tgctgccggt gaccgatgca agcctgctgg cacgccacgt
     7021 gacggggctg atcctggtgg tgcgcgtggg gagcacgacg cgcggggcga tcgagcggac
     7081 gcaggagatg ctggccgaga cccacctgcc gatcctcggg tgcattctga acgacttcaa
     7141 cgatcacgcc gccccggggg gttattatca taaagcctat ggccgggccg aggcggtggc
     7201 ggccgacgaa cagggatgag cggcgccgcc gcggccgaag cgctgttctg ggcgggcgcc
     7261 ctgtttattc tctatacgtg gttcggttat ccgctgctga tggcgggctg ggcgcgggcg
     7321 cggccgcggc cggtgcggcg cgggcgcggg caccagccga cgatgacggc ggtggtggcg
     7381 gcgcgcaatg aagcggccgg gatcgcggcg cgcgcgcgga acctgctcga gcaggactat
     7441 ccgactgagt tacttgacat cgttctcgtg tcggacggat cgaccgacgc gacggcggaa
     7501 atcatccagg ccctcgcggc ggccgagccg cgcgtgcggg cgatcgttct gccggagaat
     7561 gtcggcaagg cggcggcgct gaacgcggcg gtggagcagg cgcgcggcga ggtgctgctg
     7621 ttcgccgacg cgcgccagcg cttcgaacgc aacgtggcgg cgatgctggc ggagaatttc
     7681 gcggatcccg cggtcggatc ggccaccggc gagctgatga tcgaacgggg cggcggcgtg
     7741 gccgagcagg tggggctgta ctggcgctac gagaaatgga tccggcgcaa cgaagcggcg
     7801 agcgggtcga tgctgggggc gaccggggcg atttacgcga tgcggcggga gctgtggcgg
     7861 ccgctggagc ggggggcgct gctggacgat tttctggcgc cgatgcgcgc ggtgctggcc
     7921 ggggcgcggg cggtcttcga tgagcgggcc attgcgtggg accggccgtc ggagcggtcg
     7981 gagcaggaat tccgccgcaa gctgcggacg ctggcgggca attaccaggc gttcgcgctg
     8041 gagccggggc tgctgctgcc atggcgcaat ccgacatggc tgcgcgtctg gtcgcacaag
     8101 gtgttccggc tgctggcgcc ctacgggctg gtgctggtgt tcggcggggc gctgtttgcg
     8161 ccgggcggat tttacggggc gctggcggtg ctgcagggag tgttctacgg actggggctg
     8221 gcggggatcg cggccgagcg ccgggggcga ccggttcgct cgcgcgtggt ccggctggcg
     8281 gcgacgttca tcgtgctgca cgccgcggcg gtggggggat tgtgggtctg gctgcgcggg
     8341 ggaagcgcgg ccgggctgtg gcggaaggcg tatcaggaga acgaagggtg aacttcagat
     8401 ctgcgcgtcg gcctcggatt gaatccggcg ggcggcggcg ggctcgactt cttcgcgctg
     8461 ggggaggcgt ccgcggaaca accagacgcg ggcgcggcgc aggtcattga gaatcagcag
     8521 caggcagggg atcaggatca gcgtcaggtt cgtggcgaag gcgacgccga aggagatcga
     8581 gatcgccatc gggatgagga actgtgcctg catgcttttc tcgaggatca acggggcaag
     8641 gccgaagctg gtggtcaccg aggtcagcat gatggcgcgg aagcggcgcg cgcccgcctc
     8701 gcacagggcg gagaagacgg ggacgccgcg ggcgatccgc tcgttgacgt tgtcgatcag
     8761 gacaatggca tcgttcacga cgatgccggc cagcgccacc atgccgaaca tgctgagcat
     8821 ggtgaggtgg tagcccatga ccatgtggcc gatgacggcg ccgatgaggc cgaacgggat
     8881 cgtgaccaag atgacgagcg gctgggcata ggagcggaag atggtggcga ggatcagaaa
     8941 gatggcgagc agggcgagcg ggaagccgat gaaaaggctc tcgagggact cctgggtttc
     9001 gcgtttctgg ccttcgatgg aagcgacgag gccggggaag tcgcgcatca ggtcggcgag
     9061 gtagcccgac tcgaggaaat cgacgatttc gtgggcgttg gcgcgggcgg tatcgacctc
     9121 ggcggtgacg gcgatgcggc gcatgccgtt gcggcggttg atggcggcgt aaccctgctc
     9181 gacgtcgaga tcggcgacgg cggcaagggg gacttcgcgg ccgtcggggg tgcggatgcg
     9241 gattgattcg atatcgccca gcgagcggcg ctggtcgcgg ggatagcgga tccagacgcg
     9301 cagttcatcc cggccgcgct ggacgcgcag ggcttcgtcg ccgtagaacc cctgacgcaa
     9361 ctggcggccg agcatttcct ccgtgaggcc gaggttgcgc gcttcgggtt tgaggcgggc
     9421 gcggacttcg cgtttgccgg gccggtagtc gtcctcgacc tgatagacgc cgtcgaactg
     9481 gttgaggcgg gccttgagcc gatcggccgc ggcgagcagg tggtccatgt cgtggcccat
     9541 cagccagact tcgatcggct tgccaccggg gccggtgtcg agcgtgtcgt agcggacgga
     9601 gacggcgccg gggatgggcc cgacggcctg ttcccagcgc gagataatct gttgaaagaa
     9661 gatgccgcgc tgctcggtgg ggagcagctc gacgtagagt gagccgatat gcgtgccgaa
     9721 ctgggcattg aaaccggtgg aggcgccgct gatcgagaag agcgcctcga ccatcggttc
     9781 gccggaggcc gtcggcagct cgcgggcgac cgacgccagc gcctgctcca tgcgctcgac
     9841 ggcctggcgc gtgatggaga gcggcgtgcc gctgggaaac tcgacctggg cgatgaggta
     9901 atcggtatcg atatcgggga gaagttcgaa tttgacgagg ccggcgcgct gaacgcccag
     9961 gcagaggaag aggaaggcga tcgcggcggc gacggtcacg tagcgccagt tgaggacatg
    10021 gcgcatgagg gggacgtaga ggccggcgat caggcgatcg aggccggcgc cgaaggcgtg
    10081 acgcaaccgg agcgcggcgc ggcgcaggcc ggtggcggtt tcatcgagag gacgaaaggg
    10141 gcgcaggtcg cgcaggtggc tgggcagcat catcagcgct tcgagcagcg atacgagcag
    10201 ggcggaacag acaacgagcg gcaggacgcg gatgaacttg cccatgacgc ccttgatgaa
    10261 gaagaggggg aggaaagcga cgatcgtggt ggtgacggcg gcgaagaccg gccatgcgac
    10321 ctcgccggcg ccctggacgg ccgcatcggc cgggccgacg ccctgacggc ggcgatggta
    10381 gatcgattcg ctgatgacga tggcgtcgtc gacgatgatg cccaggatca tgatcatcgc
    10441 gaacaggctg atcatgttga tcgtctggcc ggtcaacccc atgatggcca gggcgccggc
    10501 gagggcgacg ggaatgccca atgagcacca gatcgcgagg cgcaggtcga ggaagagcca
    10561 gaggagcagg aagacgatga tcaggccgga gatgccgttg ccgacgagca tgtcgatgcg
    10621 ctgggtgatg aagatcgagc tatcaaacca gacgtgcatg ccgacgccgg cgggcaactg
    10681 gcggagcttg tggtcgatgt actcctggac ctgttcccgg attttgaggg cgtcctcctc
    10741 gttggtcttg ctgacgttga tcaggacggc gggctcgccg ttgaagcggg cgtagatcgg
    10801 atcctcgacg aaactgtcgt ggatttcggc gacctggcgc aggcggatcg aggcgccgtc
    10861 gggctgggtc agcaggatga tgtcgccgaa ctcctggccg gtgtagcggc gggccatcgt
    10921 gcggagggtc agctgttcgg cctcgccgcg cagcgagccg ccgggccagt tctggctggc
    10981 ctcgcggacg atgcggctga cctggtcgaa cgtcaggccg tagcggcgga gcttttcatc
    11041 ggacagctcg atggaaatct catattcgcg ggcgccggtg acgctgacct gcgtgatctc
    11101 gtcgcgcagc atcaactcat ccttgacctc ttcggccagc tccttgagct ggcgctcggg
    11161 gagctggccg tggatcgcga tgaacaggac ggggctgcgg gtggtgatct cggtgatgat
    11221 cgggcgttcg gcatcggcgg ggaaggtggt gatgnnnnnn nnnnatggcg ttgacacggt
    11281 cggtgatttc gtccttgagc ttctggacgt cgtaaccctc gagcacctcg atggtggcgg
    11341 tgccgacgaa ctcggtggcg gtggtgtgga aattcttaat gccctccagg ccctcgatcg
    11401 cgtcctcgag tttgagggcg atcccttcct cgacctcctc ggggccggcg ccgggatagg
    11461 ggacggtgac ctgaatgata tcgacggaaa actcgggcat gacctcgcgg accatggtga
    11521 aggcggccac gccgccgctg atgatcacga tccagaagag gatgttgacg aagacgggat
    11581 tggccacgcc ccaggccgag agctgtttca cggcgcggtg acctccagct cgcgggggcg
    11641 ctcgtcgcgt tcgatggggg gcgcgggaac gggcgcgccg ggcgaggcgg cggcgtcgtc
    11701 ccacagcgtc tgggggtcga ccagatcaac gtcgacgagg gtgccatcga ggacgatgcc
    11761 gggttgggtc gtgaggacga tctggccggg ctggaggccg tcgtcgatca ggacggtgtc
    11821 gccctcgtag cgggcgatgt ggaccgcgcg cgagtggatg cggctgtcgt tgacgatgag
    11881 gacgttgcca tcggggccga cggccgagcg ggggaggcgg tagacgccgg caacttcgcg
    11941 gccggggatc tcgacctggc agaacatccc ctcgacgagg gggaagtcgg acggggcggg
    12001 ggactgtccg tcgagcggcc gggtgacgcg ggcgacgacg tgaacggtgc gggtctgggg
    12061 atcgaagcgt tcgatgcggt cgaggcgacc ggcccactgg acgcggtcgg gctgctcggt
    12121 ccagcggatg acgacgggcc ggccctcgag ccggctgaac caggtcggct ggtcggggag
    12181 ggactggacg tcgagccagc gggcgacctc gcccccctcg agcgggaccg ggatttcgag
    12241 cgaggcgtcg tcgacgaggg tgacggcgtt ggccatggag gggccgacaa gttggccgat
    12301 ctcgatgctc ttgcgatcga cgcggccggt gaagggggcg gtgacgcggg tgcgttccag
    12361 gtcgaggcgg gcctgctcga ggcgggcgcg ggcgctctgg agctgggcgt cgaggtcgcg
    12421 gagttgggcg gggtagagcg ccagggcgtt ttcgagggcg acgacctgct cgcgcgcctg
    12481 attcatggca gtttcggcct gatccatccg cgcgggcgac tcgacgcggt cgacctcgac
    12541 caactggcgg aagcgctcga agtcgcggcg ggcgagttcg agggttcggc gggcgatctc
    12601 gatgcggcgc tggtcgtttt cccgggcctg ggccaggcgc gccttctggg cgccgaggct
    12661 gtcgagcgcg gcccgggcct gctcgacggc cgcttcgtaa tcgcgcgggt cgatgcggaa
    12721 gagggtctcg ccccgatcaa tgtagcgccc tacgtcgagg tcgggatgga cctcgatgat
    12781 gcggccggca acctggggga tgagctcgac gcggcgatcg gcgcgcgcct gaccaaaacc
    12841 ggtcaggagg gtgcggaggg tgagcgggtc gacgcggagg gcttcgacgc ggaggcggcg
    12901 ctcgaccgtt tccgcctgag ggggcggctt gcgaagcgcg gcgaggcggt tcatgacgac
    12961 ggcgccaccg ccgatcagga gcacgaccag gacggcgcgg acgatcaccg aacggatgcg
    13021 caagggcggt tacctcggac gcgcggcagt tgatccaccc gatggttgct ggtcaaacag
    13081 atcgtcgagg gcggcgcagg cgcgccgcca gtgattggcc gggcccggac cgaggcgttc
    13141 gcaaagggcg tcgatgcgct tgaggatcag cccctcgtgg cgctggacgc ccttcacggc
    13201 gcggggagtg aggctgagct ggatgcgccg gcgatcgttg cggtcggggc ggcgctcgac
    13261 cagccccagg tcgacgaggc gctcgaccat ttcggacgtc gaggcggcgg tgatttcgag
    13321 ggcgtcggcg agctccttga gactgcacgg accgatctgg cgcaggacca tcatcgtccg
    13381 gaattgagga aacgagacgt cgtggccgag gtccttccat gccttggcgt ggtccagatc
    13441 gcggtggagg aacgccatca cgcggaggag gttgcggtag atcgtgccgg cggtcgggga
    13501 gtcgtgcttg gcggtcactg ttcccgtctc cttggggtag tccggtgggc cgttggggac
    13561 ggcccggcgg ctcccttgat tctaaggact cctgattatc aggtcaagct gcgggtgggg
    13621 gttgcagcag ttccgcaaga tgtgtcgtgg ggaggtgatc ggggttgctg ggagggct
//
LOCUS       DAOPCO010000080        13667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4503_length_13667_cov_5.157067, whole genome shotgun sequence.
ACCESSION   DAOPCO010000080 DAOPCO010000000
VERSION     DAOPCO010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4503_length_13667_cov_5.157067"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1202)
                     /locus_tag="PLS90_07490"
     CDS             complement(39..1202)
                     /locus_tag="PLS90_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02288.1"
                     /translation="MTEPLPAAPPPAGMAPAGEGRRRRRLSSPRLWVGLLVTAGWLAV
                     MFTLWRNESTRPGSTLRQMGIAPEVLMVEFGGYSHVLWIQQAGRRLGATWLSIVQEES
                     PVASAAVEEGASPGSTPPAAVQATLPKYDLESLTYLEARMLGLRVPLTLAIRVRMNVA
                     FELETIQAQADALGRRLELSAFTEGERLFYRIRVEGDESATTATTAATALWHLPAANG
                     GELLDGGSALAGEIDLCGSAPLESPVVLSDVILPIVSRRRQLAAGQRWSTRVDHPLFG
                     LYHLNLDVEVEGLETIEFDGGKIEAWRMTERVGPSSSTVWYDQEGQLLRRVMEGGIVM
                     ERATAADVRQWRPSFGRMPVFEELDREYIRGHLEPHLTDRSLAGVLPDLPGLR"
     gene            1395..2096
                     /locus_tag="PLS90_07495"
     CDS             1395..2096
                     /locus_tag="PLS90_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02289.1"
                     /translation="MKWLSGLLALLLMSLALEGAAAAEQGDPAPPVVKPMLKLDGATT
                     TTLSPALIRRQRAMLRGTQGAYTTRPIVVAGTFLSYDDATSSVLVAIDRQRSSLPNIL
                     RRRAQNASLEQELLDRELPPQRAYRISRRTLFLDGRASAKKPESPASEGRVADDPQRI
                     DFDQFKPGDPVSLLYRMTNDPDEMPTLINLSLVDPGQTDFRADFSPSAGKPVYRLIPF
                     NQNDPTTSTATPDSP"
     gene            2108..3958
                     /gene="mrdA"
                     /locus_tag="PLS90_07500"
     CDS             2108..3958
                     /gene="mrdA"
                     /locus_tag="PLS90_07500"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPK02290.1"
                     /translation="MNASVRSAPLGAHDSAAHDEPTRDELPFIEKRLALLFGCFVMVM
                     TLFGLQSYKLTVIEGSVYTDRSENNFLSELPLIAPRGQILDRYGAPLAINEPSFEIRM
                     SPFRLKPDEIRATGRRVAELLGRPDINQRAEKVLSLAYAWQSTSLVQNLSLDQVLPIQ
                     EQGFMLPGVIITPHTRRVYPAGELTGHITGHVGHISPEEKAQFRARGYLNQELVGKLA
                     AELAFEDLLHGRAGSEIVERDAYGRPRSSYVDQPAQPGNALYLTLDLGLQRLAHALLG
                     DYHGAIIVMDPRDGAVLAAVDRPGYDPNRPTRGQQYSKLILSPSAKGFPPGSTFKVVT
                     AAAGLTAGFAPTDSVNCDGAYHLAGIRFPCHLRWGHDWENLYDALQHSCNVFFYRWAH
                     LAGVRNMAAMAAGFGFGQPTGFELAPPGKEGGATLVNPNLTRVPTGKVLQMGIGQGEL
                     IGVTPIQMARAYAALCNGGKLFKPRILHEVRTAQGERVRVGQPEEQGALPLAEEHRRQ
                     ILEGLRRVAQEPGGTGSRAGFKKAWRVAGKTGTAETGIRGKPSNAWFCGFAPFENPEV
                     LVIVMVEQAGHGGDVAAPLARQLLAFYFGEPEPVIQPAIGEDKKPAQSGD"
     gene            4191..4967
                     /locus_tag="PLS90_07505"
     CDS             4191..4967
                     /locus_tag="PLS90_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPK02291.1"
                     /translation="MHLLHFPSSPLPDSWLHRVDPRAKLLLVLCVACPCLLLPYRATL
                     GLLPLAAFPLLVLVRSGCPPRAIARAWLTPVPFVLFLAAWHPWLDPSPAWLAGWPGEL
                     TAGWVRFWRILLSALVLIGFLLALVATTSAPALLSALARLGAPRWLVLILGFNLRYLA
                     LLLRRGADLRAARALRAAGGAPLSRNLRLSAGMLTQFLARTLADSEQVEHALTLRGFD
                     GRCPTLGAGPLRRLDFAVIAAGLAIALLPWAASWLAGFAR"
     gene            4964..5764
                     /locus_tag="PLS90_07510"
     CDS             4964..5764
                     /locus_tag="PLS90_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02292.1"
                     /translation="MIPPRILIRRLRFGYPGQPELFHGLDLDVQPGEALGIIGSNGCG
                     KSTLLCLLVGLHDSGGSIHFDDTPLTPPNLAALRRRVGLVFQDSETQLFMPTVLEDVA
                     FGLLNQGLAPAQAEARARDMLRQIGLESLADRPPHQLSGGEQRRAALAGVLVMQPDVL
                     LLDEPSLNLDPRGRRQLIELLDDLPRRLNRPVTRVITSHDLDLVWDLCDRAALLDGGQ
                     IVASVPPDQLLRDADLLAAHGLEPPLSLLARPRILPASPPDQEDRQCI"
     gene            5755..6387
                     /locus_tag="PLS90_07515"
     CDS             5755..6387
                     /locus_tag="PLS90_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPK02293.1"
                     /translation="MHLTDHVIPLDRPAGAVICLAAAGLTVVGLALGARRLDPREIPR
                     LGLLTAWVFVASSISIPLGGLSVHLGLYGLAGFLLRGRIMLCVFTALLLQLVLLQHGG
                     VSTLGVNTLILSFGGFAGWALAAAPVGSLPLRAGLAGALGIFLPALLLVGLLRAVGYP
                     ALVGSLLAAYGGLALVEACLTAMIVQGLSRIHPGALDLAPPPNSTTECAP"
     gene            6384..6839
                     /locus_tag="PLS90_07520"
     CDS             6384..6839
                     /locus_tag="PLS90_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02294.1"
                     /translation="MKSFRGLLLAAGVWSIAICAAAHGLHLSVDVRADHVRIAGAYEN
                     GTPLAGATVAIFAPQREEPYQEGRTDPAGRFAFIPDAGGRWRIRLDDSSGHVLERQVD
                     IPAAGSPAASGPAAPPSPAANFHTFGLLVGVGLLAGISALAFWRTSTSK"
     gene            7049..8287
                     /locus_tag="PLS90_07525"
     CDS             7049..8287
                     /locus_tag="PLS90_07525"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK02295.1"
                     /translation="MKRSIPSPGAMAIGLLLALALQGCHTLSRNGETVAMRSHGDELY
                     RMAPPSVETRWFTFENPQGLKGEGGQARFGRKGSPATAIPAGEALTLVDVEGSGTIRK
                     IWLALGSRQLEEQRGVSIEMYWDHAATPAVQAPAGDFFCHSLGHATPFQSALFASPEG
                     RSHVCFVPMPFRKHARILLRNETEKPVWVYYDVAVTLGEQHDDDMLYFHSTWRRQNPT
                     ILREDFTILPKVTGRGRFLGCHIGVRLNPQVSNMWWGEGEVKMYLDGDEEFPTLCGTG
                     TEDYILDAYGQGLFDNLYMGNHYVAPDNSAYGFYRFHIPDPVWFHQDLRVTIQVMSGP
                     GYPAMLEAMAKTPGLRFMKPTDGTEYFTEEELRAHPDRSSVVEREGDDYCATAYFYLD
                     RPENDLPPLADVRERLADLY"
     gene            8501..8845
                     /gene="rplS"
                     /locus_tag="PLS90_07530"
                     /pseudo
     CDS             8501..8845
                     /gene="rplS"
                     /locus_tag="PLS90_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            9136..9885
                     /gene="fabG"
                     /locus_tag="PLS90_07535"
     CDS             9136..9885
                     /gene="fabG"
                     /locus_tag="PLS90_07535"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPK02296.1"
                     /translation="MGETLKGQVAVVTGGARGIGRAIAEALAAEGATLVLADLNADGA
                     VAAAGEVARDFAVAAQGLGCDVSRSDACQALIDQAVQTHGRLDILVNNAGITRDKLLP
                     RMTDEDWQLVLSVNLNSAFYCSKAAARVMTRARSGRIISIASVVGLMGNAGQCNYSAS
                     KAGLIGFSKSLAREIGGRGVTVNCVAPGFIDTAMTQVLGDDIKEALLKQIPLGKLGTP
                     QDVAAAVVFLASPAAAYITGEVIRVDGGMAM"
     gene            9904..11478
                     /locus_tag="PLS90_07540"
     CDS             9904..11478
                     /locus_tag="PLS90_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02297.1"
                     /translation="MRAQRPLSDGPRPQPHRLAGAGASLFSRALAPLLAILLLAAAAL
                     AFGNLAAYIQIPRPLTFPEGAVVYGAVQVASGEPLYADWRQWPHRFAPYGPLLYAPVG
                     AWMAAAGAPPFAFAYYLAGRIQSLAALCAIALVLSGLARRLGCGWIAAAACGAGMTAL
                     WPTLLGYLASFRPDAPMLCFSLLAVWLALGDGLARPRRRWLILAALWIALLYKITAWS
                     APLVVGFIAWNTLGRRQAVRWAAVYLLGVALIFSLLHLVTGGYSTINILGSQLNGFTP
                     PPFLTPAVSEWGLDRALVMLRIFGVLFIGAWLVLRNRHEPAARAIGLFGVLATLCAAI
                     QGTKVGADINYLAEPFFLAAPLAGWVWSRVLNDPRSPGGRRAAATLALALLVLLPAWR
                     GGRSYLDQWRRIRHVPPERFAVTRMDQLSPPVLLMDAAFTHPDPEAHAVPDPFHYALM
                     VGHGRISPRPLSTRLSAGAYDFVALTAIARSALDAEPACVSVRAALEQAYDLKQQLAE
                     IALWQRRATQPPAIDE"
     gene            complement(11486..12289)
                     /locus_tag="PLS90_07545"
     CDS             complement(11486..12289)
                     /locus_tag="PLS90_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPK02298.1"
                     /translation="MRILFLGDIFAKPGRRAVSHWLPGFRAEAGLDFVIANGENAAGG
                     KGLTHETAHELFEAGVDALTGGNHSFQHRESYSLFDQDKRVLRPGNLPPGAPGRGLGI
                     YDLTSGGRIAVINLQGRAFMKAHDCPYRIADSLIAEARMETAVIVIDFHAEATSEKVA
                     MAAYVDGRVTAVIGTHTHIPTADARLTAAGTAAITDVGMTGPYDSVIGIESGVVLEQL
                     MSGMPVRHRVAEHGVRICGLMFDAELESGRATNVEPLVLPPWPRGLPPT"
     gene            complement(12564..>13667)
                     /locus_tag="PLS90_07550"
     CDS             complement(12564..>13667)
                     /locus_tag="PLS90_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPK02299.1"
                     /translation="NPMAVSAGAFIALATDHIIMGRNSSIGGALPLTIGAEGVGAAEK
                     KFQSVFNAEIRKTARTKGHPEDIAEGFSNPDLVIPGLKKEGDILTLTFDEATSLGLAE
                     YQAETLDELLAREGLSGARVVRFQLTTTDQIARFLSSSAVLGVLMMVGLGALVLELKT
                     PGVGLPGAIALLAFGLAFGGSYLANLSGYMEVIFFFVGVVLLVVEIYVLPGFGIAGIL
                     GILCVVGSLLFALFSFAPDGFSLTAPRLDMLGSAVLTVTAALVGLIPFFIVLGKILPA
                     TPLYRNLMLGPEGRESPAPAETAGAAAAPRAEAAVAPGQTGRALTDLRPAGIALLGEA
                     RVDVLTQGEYLEKGTPVVVVRVEGARVFVARGE"
BASE COUNT         2067 a         5380 c         3969 g         2251 t
ORIGIN      
        1 ccacctcccc ccccgcttcc cgtcagggat gccaggcctc accgcagccc cggcagatcc
       61 ggcaacaccc ccgccagcga ccggtcggtc agatggggct ccaggtgccc ccgaatgtat
      121 tcgcggtcaa gctcctcgaa caccggcatc cgcccgaacg acggacgcca ctggcgcacg
      181 tcggccgccg tggcccgctc catcacgatc cccccctcca tcacgcgccg caagagctgc
      241 ccctcctggt cataccacac ggtcgagctg gacgggccca cgcgttcggt catgcgccac
      301 gcttcgatct tccccccatc gaactcgatc gtctccaacc cctctacctc gacatccaga
      361 ttcaggtgat acagcccgaa cagcggatga tcgacgcgcg tcgaccagcg ctggccggcc
      421 gccagctgcc gccgccgcga gacgatcggc aggatcacgt cgctcaacac gacggggctt
      481 tccagcggcg cgctgccgca cagatcaatc tcccccgcca gcgccgaccc gccgtcgagc
      541 aactcgccgc cgttcgccgc cggcagatgc cacagcgccg tcgccgcggt cgtcgccgtg
      601 gtcgcacttt cgtccccctc gactcgaatc cggtagaaca gccgctctcc ctcggtgaag
      661 gcgctcagct ccagtcgccg ccccagcgca tcggcctggg cctggatcgt ctccagttca
      721 aacgccacgt tcatacgcac ccggatcgcg agcgtcaatg gcacccgcag ccccagcatc
      781 cgcgcttcca gataggtcag cgattccaga tcatatttcg gcagcgtcgc ctggaccgcg
      841 gcgggcgggg ttgaccccgg cgacgccccc tcctcgaccg cggccgacgc gaccggcgac
      901 tcttcctgca cgatcgacag ccacgtcgcc cccagccgcc ggcccgcctg ctggatccac
      961 agcacatgcg agtagccgcc gaactcgacc atcagcactt cgggcgcaat ccccatctgg
     1021 cgcaacgtcg accccggccg cgtcgattcg ttgcgccata atgtgaacat cacggccagc
     1081 cagccggccg tcaccagcag ccctacccac agccgcggcg aactcaatcg ccggcgccgg
     1141 cgcccttccc cggccggcgc cattccggcg ggtggcgggg cggcgggcag gggttcggtc
     1201 atggcggcgc gtcccgtctt agaaactcgg cgcggcgatt tcagacgcgg cgccattgtg
     1261 acccaaaccg cgccaaagca aaagcgattg cattggcgat gcccgccccc ggccaactgt
     1321 gatacagtgt gaccgccgcc gaacgccccc tgcgggatca cgaccggcgg cattttgcga
     1381 cggaggatgt ttcgatgaag tggttgtccg ggctgctggc gctgctgctc atgagtctcg
     1441 ccctcgaagg cgccgccgcg gcggagcagg gggaccccgc ccccccggtc gtcaagccga
     1501 tgctgaagct cgacggcgcg acgacgacga cgctctcgcc ggcgctgatc cgccgccagc
     1561 gggccatgct gcgcggcact cagggcgcct acaccacccg cccgatcgtc gtcgccggca
     1621 ccttcctcag ctacgacgac gcgacctcca gcgttctggt tgcgatcgac cgccagcgct
     1681 ccagcctgcc caacatcctc cggcgccgcg cgcaaaatgc cagtctcgaa caggaattac
     1741 tcgaccgcga actgcccccc cagcgggcct atcgcatcag ccgccgcacc ctcttcctcg
     1801 atggccgcgc cagcgcaaag aaaccggagt cccccgccag cgagggtcgc gtcgccgatg
     1861 acccccagcg catcgacttt gatcaattca agccgggcga tcccgtctcg ctcctctatc
     1921 ggatgacgaa cgatccggac gagatgccca cgctcatcaa cctctcgttg gtcgaccccg
     1981 gccagaccga tttccgcgcc gacttcagtc cttccgccgg caaaccggtc tatcgcctga
     2041 ttcccttcaa tcagaacgac cccacaacct ccacagccac gcccgattcc ccctagcggt
     2101 tccgcccgtg aacgcctcgg ttcgctcagc ccccctcggc gcccacgact ccgccgccca
     2161 cgacgagccg acccgcgatg aactcccgtt catcgagaag cgcctcgccc tgctcttcgg
     2221 ctgcttcgtc atggtgatga cgctctttgg cttgcagtcc tacaaactga cggtcatcga
     2281 agggagcgtc tacaccgatc gctcggagaa taactttctg agtgaattgc ccctcatcgc
     2341 cccccgcggc cagatcctcg atcgctacgg cgcgccgctg gcgatcaacg aaccctcctt
     2401 cgaaatccgc atgagcccct tccgcctcaa gccggacgag attcgcgcca ccggccgccg
     2461 cgtcgccgaa ctccttggtc gacccgacat caaccagcgc gccgaaaaag tcctctcgct
     2521 ggcctacgcc tggcagagca cctccctggt gcagaatctc tcgctcgatc aggtccttcc
     2581 catccaggaa cagggcttca tgctccccgg cgtcatcatc accccccaca cgcgccgcgt
     2641 gtatcccgcc ggcgaactga ccggccatat caccggccat gtcggccaca tcagccccga
     2701 ggaaaaggcc caattccgcg ccaggggcta tctcaatcag gaactggtcg gcaagctcgc
     2761 cgctgaactt gccttcgagg acctgctcca cggccgcgcg gggagcgaga tcgtcgagcg
     2821 cgacgcctac ggccgtcccc gttcaagcta cgtcgaccag ccggcccagc ccggcaatgc
     2881 gctctacctg acgctcgacc tcggcttgca gcggctcgcg catgccctcc tgggcgacta
     2941 ccacggcgcc atcatcgtca tggatccgcg cgatggggcg gtgcttgccg ccgtcgaccg
     3001 acccggctat gaccccaacc gcccgacccg cggccagcaa tactccaaac tcatcctcag
     3061 cccgtcggcc aagggttttc cccccggctc gaccttcaag gtcgtcaccg ccgccgccgg
     3121 cctgaccgcc ggcttcgccc ccaccgacag cgtcaactgc gatggcgcct accatctggc
     3181 cggcatccgc ttcccctgtc acctgcgctg gggccacgac tgggaaaacc tctacgacgc
     3241 cctgcaacac tcctgcaacg tcttcttcta ccgctgggcc cacctggcgg gcgtgcgcaa
     3301 catggcggcg atggcggccg gtttcggctt tggccagccg accggtttcg aactcgcgcc
     3361 cccgggcaag gagggcggcg ccacgctggt caaccccaac ctcacccgcg tccccaccgg
     3421 caaggtcctc cagatgggga tcggccaggg cgaactgatc ggcgtcaccc cgatccagat
     3481 ggcccgcgct tacgccgccc tctgcaatgg cggcaagctg ttcaagccgc gcatcctgca
     3541 cgaggtccgc acggcccagg gcgaacgcgt ccgcgtcggc cagcccgagg agcagggcgc
     3601 cctccccctg gccgaggaac accgccgcca gatcctcgaa ggcttgcggc gcgtcgccca
     3661 ggagcccggc ggcaccggtt cgcgcgccgg attcaaaaag gcgtggcgcg tcgccggcaa
     3721 aaccggcacc gccgaaaccg gcatccgcgg caagccctcc aacgcctggt tctgcggctt
     3781 tgcccccttc gaaaaccccg aggtcctggt catcgtgatg gtcgaacagg ccggccacgg
     3841 cggcgacgtc gccgctcccc tcgcccgtca gctcctcgcc ttctactttg gcgagcccga
     3901 accggtcatc cagcccgcca tcggcgagga caaaaaaccc gctcaatcag gcgactaaga
     3961 ccacacggca atcgagaaat ccctctcgtg tggggcgccc tcctgatcac ccccgcaatc
     4021 ctggatcata ccagccagtg tagggccggc gccctcgccg ggctccccac cgcatcccgg
     4081 agggaagcca ggggccagat ccctctttcc gctggcgcgt cacacgaatt cttgataagt
     4141 gtgaatccag cggcccccac cccgtccgcg cttcgggccc ccgcgtcgcc atgcacctcc
     4201 ttcacttccc ctcatccccc ctccctgact cgtggcttca ccgcgtcgat ccccgcgcca
     4261 agctcctcct cgtcctctgc gtcgcctgcc cctgcctctt gctcccttat cgcgccacgc
     4321 tcggcctcct tccgctggcc gccttcccgc tcctggttct ggtccgatcc ggctgccccc
     4381 cgcgcgccat cgcccgggcc tggctgacgc cggtgccgtt cgtcctcttc ctcgccgcgt
     4441 ggcacccgtg gctcgatccc tcgcccgcct ggctggccgg ctggcccggc gagctgaccg
     4501 ccggctgggt gcgtttctgg cgtattctcc tgagcgccct cgtgctcatc ggctttctcc
     4561 tcgccctggt cgccaccacc tccgccccag cgctgctcag tgctctggcg cgcctgggcg
     4621 ccccgcgctg gctcgtcctg atcctgggct tcaacctgcg ctacctggcg ttgctcctcc
     4681 gccgcggcgc cgatctgcgc gcggcgcgcg cactgcgcgc cgccggcggc gctcccctct
     4741 cccgcaacct gcgcctctcg gccggcatgc tgactcaatt cctcgcccgc acgctggcgg
     4801 actccgaaca ggtggagcac gctctcacgc tgcgcgggtt cgatggtcgc tgcccgacgc
     4861 tcggcgccgg ccccctccgc cgcctcgact tcgccgtcat cgccgccggc ctcgccatcg
     4921 cactgcttcc atgggccgca agctggctcg cggggtttgc ccgatgatcc ccccgcgcat
     4981 ccttatccgc cgactgcgct tcggttatcc cggccagccc gagctgttcc acggcctcga
     5041 cctggacgtc cagcccggcg aagccctcgg catcatcggc tccaacggct gcggcaaatc
     5101 gaccctcctg tgcctcctcg tcggtctgca tgattcgggc gggtcaattc actttgatga
     5161 cacgccgctc acgccgccca acctcgccgc actgcgccgc cgcgtcggcc tcgtattcca
     5221 ggattccgaa acccaactct tcatgccgac cgtcctcgag gacgtcgcct tcggcctgct
     5281 caatcagggc ctggccccgg ctcaggccga ggcccgcgcc cgcgacatgc tccgccagat
     5341 cggcctggaa tcgctcgccg accggccgcc gcatcaactc tccgggggcg agcaacgccg
     5401 cgccgccctg gccggcgtcc tcgtcatgca gcccgacgtc ctgctgctcg atgaacccag
     5461 cctgaacctc gatccgcgcg gccgccgtca gttgatcgag ctcctggacg acctcccccg
     5521 gcgcctcaac cgccccgtca cccgagtcat cacctcccac gatctggatc tggtgtggga
     5581 cctgtgcgac cgcgcggcgc tgctggacgg cgggcaaatc gtcgcctcgg ttccgcccga
     5641 tcagctcctc cgcgacgccg acctcctcgc cgcgcacggc ttggaaccgc cgctcagcct
     5701 gctcgcccgg ccgcgcatcc tgccggcgtc gccgcccgac caagaggacc gccagtgcat
     5761 ctgaccgacc acgtcatccc cttggatcgc ccggccggcg cggtgatctg cctggccgcc
     5821 gctggcctga ccgttgtcgg tctcgccctc ggcgcgcggc ggctcgatcc ccgcgaaatc
     5881 ccccgcctgg gcctcctcac cgcctgggtt ttcgtcgcct cgtcgatcag catccccctg
     5941 ggcggcctca gcgtgcatct cggtctctac ggcctggcgg gcttcctcct ccgcggccgg
     6001 atcatgctct gcgtcttcac cgcgctcctg ctccagttgg tactgctcca gcatgggggc
     6061 gtctccacgc tcggggttaa cacgctgatt ctctccttcg gcgggttcgc cggttgggcc
     6121 ctcgccgccg cgccggtggg atcgctgccg ctgcgcgcgg gcctggccgg cgcgctgggg
     6181 atttttctgc cggcccttct cctcgtcggt ctcctccggg ccgtcggcta cccggcgctt
     6241 gtgggttcgc tcctggcggc ctacggcggg ctcgcccttg tcgaggcctg cctgaccgcc
     6301 atgatcgtcc agggactcag ccggattcac cccggcgcgc tggacttggc gccaccgccg
     6361 aattccacca cggagtgcgc cccgtgaagt cgtttcgagg gctgctgctg gccgccggcg
     6421 tgtggagcat cgccatctgc gccgcggcgc atggattgca tctgtcggtc gatgtccgcg
     6481 ccgatcatgt gcggattgcc ggcgcctatg agaacggaac accgctcgcc ggcgcgaccg
     6541 tcgccatctt cgccccccag cgcgaggagc cctatcagga agggcggacc gatcccgccg
     6601 gccggttcgc attcatcccc gacgccggcg ggcgctggcg catccggctt gatgactcct
     6661 ctggccacgt gctggaacgg caggtggaca tccccgcagc cggctcgccg gcggcttccg
     6721 gtccggccgc gcccccctcg ccggccgcca attttcacac gttcgggttg cttgtcggcg
     6781 tcgggctcct ggccggcatt tcggctctgg ctttctggag aacgagcact tccaagtaat
     6841 ccgcccgcgg tcgggcgccg ggagttggcg ggagggcccg gccttctccc tcgctggcgg
     6901 gcagcgcatg actttcagga ttgaagcctc tgaaaattca tgcaggatag ctgccgttgc
     6961 aagtcgccgc cgcttcctgg cgcggcgcgg caaggccggg cgccgtcggc ccaccgacca
     7021 gtctcaaacc aaaacacgag gtgctgccat gaagcgaagc atcccgtccc cgggggcgat
     7081 ggcgatcggc ttgctgctcg ccctcgcctt gcagggctgc catactctga gccgtaatgg
     7141 agaaaccgtc gcgatgcgat cacacgggga tgagctctac cggatggcgc cgccgtccgt
     7201 cgagacaagg tggttcacct tcgaaaatcc gcagggattg aagggagaag gcgggcaggc
     7261 gcggttcggc cgcaaaggat cgcccgccac cgcgatcccc gcgggcgaag ccctgacgct
     7321 ggtcgatgtc gagggctccg ggacgatccg gaaaatctgg ctcgcgctcg gatcgcgcca
     7381 gctggaggag caacgcggcg tcagcatcga gatgtactgg gatcacgcgg cgactcccgc
     7441 cgtgcaggcc cccgccggcg atttcttctg ccacagtctg gggcatgcca ccccgtttca
     7501 gagcgcgctg ttcgcctccc ccgagggccg ctcgcatgtc tgcttcgtcc cgatgccgtt
     7561 ccgcaagcac gcccgcattc tgctccggaa cgaaaccgaa aagcccgtct gggtctacta
     7621 cgacgtggcg gtcacgctgg gcgagcaaca tgacgacgac atgctctact tccacagcac
     7681 ctggcgccgg caaaacccga cgatcctgcg cgaagacttc accatcctgc ccaaggtcac
     7741 cggccgcggc cggttccttg gctgccatat tggagttcgc ttgaacccgc aagtcagcaa
     7801 catgtggtgg ggcgagggcg aggtgaagat gtatctcgat ggcgacgagg agtttccgac
     7861 cctttgcggc accggcaccg aggactatat cctcgacgcg tacggtcagg gtctgttcga
     7921 taacctctac atggggaacc attacgtcgc ccctgataat tcggcctacg gcttctatcg
     7981 cttccacatt cctgatccgg tctggttcca tcaggacctg cgcgtcacga tccaggtgat
     8041 gagcggcccc ggctatccgg ccatgctcga agccatggcc aaaacccccg gcctgcgctt
     8101 catgaagccg accgacggca cggagtactt caccgaggag gagttgcgcg cgcaccccga
     8161 ccgcagcagc gtcgtcgagc gggaaggcga cgattactgc gcgaccgctt acttctatct
     8221 ggatcgaccg gaaaacgacc tgcccccgct ggccgacgtg cgcgaacgac tggccgatct
     8281 gtattaaatg ccagccctcc gggcgcaatc gggggagcgg ccaccgcacg attttcgctt
     8341 gcccgccgcc cgccggcttg ctactttaac ccgttctatg ccttggcggg gagaactgcg
     8401 tccgccgggc ttggggggcc ggacgggctg cccgtcgcaa cgcgcatcct tcccatcaga
     8461 tgctttacgc ctcgtgaagg cgaccgggag ttgaagaacc atgaacgcgc tcgtgcagca
     8521 ggttgaacgc aaatacctca agaaatccgt gccggacttc cgccccggcg acacagtccg
     8581 cgtcgccgtc cgcatcgtcg aaggcaaccg cgagcggatc cagaatttcg aaggcgtctg
     8641 catcgcccgc catggcgccg gcatgagcga aacgttctcg gtccgcaagg tctccttcgg
     8701 cgtcggcatg gaacggacct tcacccttca ctccccgcgc gtcgaggcga tcaaggtcct
     8761 gcgccagggc cgcgtccgcc gcgccaagct ctactatctg cgcgacctct cgggcaagaa
     8821 ggcccgcatc gccgacaccc gccgcaagag gagcggcaag gacgcgatga tcgagctcct
     8881 cgccggtgag ggtttgacgc ccgagcccgc tccggaggag gcgagcgccc aatccgccgc
     8941 cgcgggcgaa gcgcccgccg aagcgtccca gcccgcctcc gccagcgaag atccgccgat
     9001 cgcctgagcc ccgacccacc tcaatcttcc ggccgccgat gattgaactt gtcggcggcc
     9061 ctttcatttg ccatcttaag ccctctccgc cgaggcatcc gatccggccg ggaatttgcg
     9121 ccaaaggagc acgttatggg tgaaacgttg aagggtcagg tggcggtcgt caccggcggc
     9181 gcccgcggca ttggccgggc catcgccgaa gcgctcgcgg ccgagggggc gacgctggtc
     9241 ctggccgatc tgaacgccga tggcgccgtc gccgccgccg gcgaggtcgc ccgcgatttc
     9301 gccgtcgccg cccagggcct cggctgcgat gtctcccgct ccgacgcctg tcaggcgctc
     9361 atcgaccagg ccgtccagac ccacggccgg ctggacatcc tcgtcaacaa cgccggcatc
     9421 acgcgcgaca aactcctgcc gcgcatgacc gacgaggact ggcaactggt cctgtcggtt
     9481 aacctgaact cggccttcta ctgctcgaag gcggcggcgc gcgtcatgac gcgcgcccgc
     9541 tcgggacgca tcatttccat cgcctcggtc gtcggcctga tgggcaacgc cggccagtgc
     9601 aattactccg catccaaggc cggcctgatc ggtttctcca agtcgctcgc ccgcgaaatc
     9661 ggcggccgcg gcgtcaccgt caattgcgtc gcccccggct tcatcgacac cgcgatgacc
     9721 caggtcctcg gcgatgacat caaggaagcc ctcctcaagc agatcccgct gggcaaactc
     9781 ggcacgcctc aggacgtggc ggcggccgtt gtcttcctcg cctccccggc cgcggcctac
     9841 atcaccggcg aggtcattcg cgtcgacggc ggcatggcga tgtagccccc ctccgctcac
     9901 ccggtgcgcg cccaacgccc actctccgac ggcccccgcc cccagccgca tcgactggcc
     9961 ggcgcggggg cttcgctttt ctcccgcgcc ctcgccccgc tcctcgcgat cctgctcctt
    10021 gccgccgcgg cgctcgcctt cggcaacctg gccgcctata tccagatccc ccggccgctc
    10081 accttccccg aaggggccgt cgtctacggc gccgtccagg tggcgtcggg cgaaccgctt
    10141 tacgccgact ggcgccagtg gccgcaccgc ttcgccccct acggaccgct cctgtatgcg
    10201 ccggtcggcg cgtggatggc cgcggcgggc gcgcccccct tcgccttcgc ttactatctg
    10261 gccggccgca ttcagtcgct cgcggcgctc tgcgccatcg ccctcgtcct gtccggcctg
    10321 gcccgccggc tcggctgcgg ctggatcgcg gcggccgcct gcggcgccgg catgaccgcc
    10381 ctctggccca cgctgctcgg ctacctcgcc agcttccggc ccgacgcccc gatgctctgc
    10441 ttcagtctgc tggccgtctg gcttgccctc ggcgacggcc tggcccgccc gcgccggcgc
    10501 tggctcatcc tggccgctct gtggatcgcg ctgctctaca agatcaccgc gtggagcgcc
    10561 ccgctggtcg tcggcttcat cgcctggaac accctcggcc ggcgacaggc cgtccgctgg
    10621 gccgcggttt acctgctggg cgtcgccctc atcttctccc tgctccacct cgtcaccggc
    10681 ggctattcca ccatcaacat cctgggctcg cagttaaacg gcttcacccc gccgcctttt
    10741 ctcaccccgg ccgtctccga gtggggcctc gatcgcgccc tcgtcatgct gcgcatcttc
    10801 ggcgtgctgt tcatcggcgc ctggctagtc ctgcgcaacc ggcacgagcc ggcggcgcgc
    10861 gcgatcggcc tgttcggcgt tctcgccacg ctctgcgcgg ccatccaggg aacgaaggtc
    10921 ggcgccgaca tcaactacct ggccgagccc ttctttctgg cggccccgct cgccggctgg
    10981 gtctggtccc gcgtcctcaa cgacccccgc tccccaggcg gacggcgcgc ggcggcgacc
    11041 ctcgccctcg ccctgctcgt cctcctcccc gcctggcgcg gcggccgctc ctacctggac
    11101 cagtggcgcc gcatccgcca tgtccctccc gagcgctttg ccgtcacccg catggaccag
    11161 ctgtcccccc cggtcctgct gatggacgcc gccttcaccc accccgaccc cgaggcgcac
    11221 gccgtccccg atccctttca ttacgcgctg atggtcggcc acggccgcat ctccccgcgg
    11281 ccgctgagca cgcggttgag cgccggcgcc tatgatttcg tcgccttgac cgcgatcgcc
    11341 cgttcggccc tcgacgccga gccggcctgc gtatccgtcc gcgcggcgct tgaacaagcc
    11401 tatgatttga aacagcaatt ggccgagatc gccctctggc aacggcgcgc gacacagccc
    11461 ccggccatcg acgaatgaat cgcccctacg tcggcggcag cccgcgcggc cacggtggca
    11521 gcacgagcgg ctccacgttc gtcgcccgcc ccgactccag ttccgcatcg aacatcagcc
    11581 cgcagatccg caccccatgc tccgccaccc gatggcgcac tggcatcccc gacatcagtt
    11641 gttccagcac caccccgctt tcaatcccga tcaccgaatc gtagggcccc gtcatcccca
    11701 catcggtgat cgccgccgtc cccgccgccg tcagccgcgc gtcagccgtc gggatatgcg
    11761 tgtgcgtccc gatcacggcc gtcacccgcc cgtccacata ggccgccatg gcgaccttct
    11821 cgctggtcgc ctccgcgtgg aaatcgatga cgatcacggc ggtttccatc cgcgcttccg
    11881 ctattagcga gtcggcgatc cggtacgggc aatcgtgcgc cttcatgaaa gcgcggccct
    11941 gcaggttgat caccgcgatc cgcccgccgc tggtcaggtc gtagatgccc agcccgcgcc
    12001 ccggcgcccc cggcggcagg ttccccggcc gcagcacccg cttgtcctga tcgaacagcg
    12061 aataactctc gcgatgctgg aacgaatgat tgccccccgt cagcgcgtcg acccccgcct
    12121 cgaacagctc gtgggccgtt tcatgcgtca ggcctttgcc cccggccgcg ttctcgccat
    12181 tggcgatgac gaaatcgagc ccggcctccg cccggaaacc gggcagccaa tggctgaccg
    12241 cccgccgccc cggcttggcg aaaatatcgc ccagaaagag aattcgcatg gatcacgacc
    12301 ggctttccag gcggctgcac cgcgcaagtt gccgagacga agccccgaaa cgacgaactc
    12361 agccccccca tcgtcgcgca actgacgatg cggagcaagc aaggaaggga aaactccggc
    12421 aacgaacgac cggacgtgcc cgcaaacgaa ccagggatca acgagacact tttttcttga
    12481 gtcccttaag tctcttgagt ccctcatgtc cattccgacg ctccggagca ggaccacgcg
    12541 gcgaagccgg tccctgaatt atgtcactcc ccccgcgcga cgaagacgcg cgcgccctcg
    12601 acgcgcacga ccacgaccgg cgttcccttc tccaggtatt ccccctgcgt cagcacatcg
    12661 acccgcgctt cgcccagcag cgcaatcccc gccggacgca gatcggtcag cgcccggccc
    12721 gtctgccccg gcgcgacggc ggcctccgcc cgcggcgccg cggccgcccc cgccgtctcc
    12781 gccggcgccg gactctcccg cccctcgggc cccagcatca agttccgata cagcggggtg
    12841 gcgggcagaa tcttccccag cacgatgaag aacggaatca gcccgaccag cgcggccgtg
    12901 accgtcagca ccgcgctgcc cagcatgtcc agccgcggcg cggtcaggct gaacccgtcg
    12961 ggcgcgaaac tgaacagcgc gaacagcagc gaccccacga cgcagaggat ccccagaatg
    13021 cccgcgatgc cgaatccggg cagcacataa atctcgacca ccagcagcac gacgcccacg
    13081 aagaagaaga tcacttccat gtaacccgac aggttggcca gatacgaacc cccgaaggcc
    13141 agtccgaatg ccagcaatgc aattgcgccc ggcagaccga cccccggcgt cttgagctca
    13201 agaaccaacg cccccagccc caccatcatc agcaccccga gcaccgccga actcgacagg
    13261 aaccgggcga tctgatcggt cgtcgtcaat tggaaccgca cgacgcgcgc gccgctcaac
    13321 ccttcccgcg ccagcagctc atccagcgtt tcggcctgat attccgccag cccgagcgac
    13381 gtcgcctcat cgaacgtcaa cgtcaggatg tccccttcct tcttcaagcc ggggatcacc
    13441 agatccgggt tgctgaaacc ttcggcgatg tcctccggat gccctttggt ccgggcggtc
    13501 ttgcggatct cggcgttaaa cacgctctgg aacttctttt cggccgcgcc gactccctcc
    13561 gccccgatcg tcagcggcag cgcgcctccg atgctgctgt tgcggcccat gatgatgtga
    13621 tcggtcgcca gcgcaatgaa cgcccccgcc gacaccgcca tcgggtt
//
LOCUS       DAOPCO010000081        13597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4529_length_13597_cov_5.614680, whole genome shotgun sequence.
ACCESSION   DAOPCO010000081 DAOPCO010000000
VERSION     DAOPCO010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_13597_cov_5.614680"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLS90_07555"
     CDS             complement(<1..265)
                     /locus_tag="PLS90_07555"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660558.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HPK02300.1"
                     /translation="MRKKISVIGGGNVGSTTALFLAEKHLGDVVLTDIVEGMPQGKGL
                     DMYETGPIHGYHSRIQGTNDYKDIAGSDVVVVTAGLPRKPGMSR"
     gene            575..2581
                     /locus_tag="PLS90_07560"
     CDS             575..2581
                     /locus_tag="PLS90_07560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02301.1"
                     /translation="MDSANQSRPGAGRRARPTRAGRRGLPLLLLLGVALLWRLMFLLA
                     IHDTPAGQWHQWDQSDMHTYLMQGRQLAAGDWLAREPYYPYHLWMSAGSPAAWRALYP
                     PHVFHQAPLLSYGLALFLRFGVDPVPWIRLLHAVLGLINTYLLYRLGRLLGGRVAGLV
                     AGLFGALYGPLLVIESQPLREPAALALTLLALLCVAAMPIRRKGGRERTRALAFGAGF
                     ATGVLALLHEAAAVVLLSAALALAWDGLVVRRAPRRFAACLALLLGGALIGFLPLLAR
                     NLAVGAPPFAQSTRSALTWAMANHPDAPWGGVRWTGPDASFFETLEGTRGRPLRVVIR
                     TLAAYEGAPWRWALNWGRRALALLIGGEASDNTSHAWFRLHVPILALSLDFRWLFALG
                     AAGLLRRGRRGGCGRPAPMLATYGLLMLAALSSVFPLGRYRLFLLPCLAPFAGRLAAQ
                     AWAARRKANWRRLGGMGALVLAVLLGQMALDRAFGAIAAPRPVDFSVAARMLTEWGNP
                     AAAAAELELAAEQGVLTPAMKVELSLAHARAALLAGAEQQALASLESAGRWLAELDEP
                     PPNASAIARESAWLKLTAADASLRDPDGAERIVQGLELRENGRAELLDLKAALAAARG
                     QFDVAIGLATEALEAARGGGDEVQARFIQRRLERYHAGRPATRP"
     gene            2664..3470
                     /locus_tag="PLS90_07565"
     CDS             2664..3470
                     /locus_tag="PLS90_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02302.1"
                     /translation="MKRNSGWYLVISLAALATAALPAPTAWAQEGPSRVLGGPRSAPA
                     GPGGDTIYIERHDGRARSDGAARIERQVPSARRFQDSDIRLNDLRDADARRLRPYRRF
                     YRDPYLRTDDERFIDHRLDPLRYRYYDHFAYPYPYGFYRYGGEPVIIIDDAVIAGGTD
                     TRGGSVTYQAGERTYSAGGEDGRRWVEGHWAEMPREDLIEGETVEVFHPAVYRQREDG
                     SLEELEEERVTREPKYRVVLTPVWIEGHYVDERGIPLEPVRVEEAPTEEP"
     gene            3623..5032
                     /locus_tag="PLS90_07570"
     CDS             3623..5032
                     /locus_tag="PLS90_07570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02303.1"
                     /translation="MDPVHIFCYCSTTVPNRFAHPIQILKTVRALAEQPGVRVTLYFV
                     SLGTKPRAIWRYYGMKPLPNVRVRGMVPSWYFRLMKLNNHLCPEQQKIFHLVRWRLAR
                     ELRRVARTERAFIYTRNENAITMMRKSADAAGIPMYLELHWFKHLDWFRKLPWNQSRE
                     VAPPPIGEYKRLLRKLKANEREVLNQADGILCLTDEIRKVLSRWNLKCPLTTLPSGVE
                     LIAPEELRANGVEPAELESDDSEEEVAAGGDLGGADDGDDSADGDAAQDEAIATTDTS
                     PPNRTPLDILYLGQLYPWKGVDLLVQAMAHLPEYRLTIVGGNRDKDKNRVKSLARELG
                     VNGRVNLVGHISHPKVKRYIRKARVCVVPLPRAGYREARFFTSPMKLFEFAAWGKVIV
                     ASDLPTLREVLTHGQNALLVRPDDPVALAEGLREALENQPLREQLAAGALKLAQDHAY
                     ENRARRILEFCRPGGKVTA"
     gene            5029..6387
                     /locus_tag="PLS90_07575"
     CDS             5029..6387
                     /locus_tag="PLS90_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02304.1"
                     /translation="MNWKERVQQWVVEPTLRGLGVPVLRQYGFEPTEPPRVLLYTASN
                     VGLGHLFRVLRTAAELRRLLPRVNLLLLTDTEHLHVTQYYGQLAVLRLPNFHYFGENF
                     REKPVGLELSKGKLRLLRYNAMLAAVHSFQPHVFLMDTAPHGKRNELYPVLEYLAAQR
                     RPPIRILQLRDVPFVPGEAERTDDARRQLTRNPDFYDYLFVAGDPQYFDLAKVYDWPK
                     KITRKLFYLGFIIPEADGDGPPAVESGTEREIERMMQRPARRIVASFGGGWEAEGLGG
                     ALLAAYAELAAQRGEPLQFYLFTGPSAEDAALAGLQARAAGRDDVLIRKFSPRFSHLL
                     ARCDLAVLQAGSTPFQILETDIPMLLYARDFKSKEQQFRAEQMIRFANVELLGEDGGQ
                     PAVLRAAMARLLDAPRVPRRTGFRYGGVRRSARAIAAMLADSRKPRRLSVEELNRICR
                     EE"
     gene            6727..9255
                     /gene="leuS"
                     /locus_tag="PLS90_07580"
     CDS             6727..9255
                     /gene="leuS"
                     /locus_tag="PLS90_07580"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPK02305.1"
                     /translation="MAIETFYPFEEVEPRWQRAWDEQGLWKTPAGAARRKYYVLEQFP
                     YPSGKLHMGHVRVYTIGDAIARLRRMQGYDVLHPMGWDSFGLPAENAAIKNDAQPAAW
                     TEQCIANMREQFHRLGISYDWEREISTCRDDYYRWNQWIFIRMWEQGLVERRKAAVNW
                     CPRCATVLANEQVINGRCWRHEDTEVEIRPLEQWFLKITDYAEELLKDLEEKLGAWPN
                     LVTAQQRNWIGRSEGALVKFTVKETGEELPVFTTRPDTLFGVTFMSVAAEHPRVREWI
                     RGRENEREVQAFINHVVMEGRAARTDEAAEKKGIALGIHAINPVNEREIPIYIANFVL
                     MEYGTGAVMAVPAHDQRDFEFARKYGIPVEVVIQPEGKTLDAACMEAAYVEPGVMTHS
                     AQFDGLPSEQAKQEITEWLARQGKGEKTVQYRLRDWLISRQRFWGTPIPFIYCEGCGL
                     VPVPLEDLPVKLPENARFGGEGNPLATVPEWLEVECERCGGKGRRETDTMDTFFDSSW
                     YYLRFTDPKNERVPFDKAAAESWMPVDIYVGGKEHAILHLLYSRFFTKVLRDMGLVTL
                     DEPFKQLLTQGMVLNTGTDPRTGETKMFKMSKSIGNVIDPNQLIAERGADVARLFILF
                     ASPPEKDLPWSEQGVEGMSRFVNRIWRHAQNHGEALRAAGGGDVTPGEITEEPDRALL
                     RMIHEATRRVTHDVGERFQFNTAISACMELFNAIGDYAPRAGDALSARLWAMATRRLA
                     VLLTPFAPHLAEELWQRVGGEGLVAQQAWPSWDEAALRVETIEIAVQVNGKVRGKVRV
                     AAEAGEEAVRAAALADETVQRFIDGKTIHKFVYVKGKLASLVVK"
     gene            complement(9280..10623)
                     /locus_tag="PLS90_07585"
     CDS             complement(9280..10623)
                     /locus_tag="PLS90_07585"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPK02306.1"
                     /translation="MADIAPFVATRYALTRSQELARVLTPPYDVITPAMQQAFYDADP
                     HNIVRIDFGKTMPDDNEFENRYARAGALWHQWRDEKVVVEDPKKCFHVYEQEFELPDG
                     RTVRRRGFYAAVRLQDFSEGGIRAHEHTFDGPKADRFRLMRATNANLSPIFCLYDDPS
                     RQVDELLAQATASKAIETRFDGVVQRLWPLCKSSVVARITERMKDQTLFIADGHHRYE
                     TSLLFRDEMREITGRRDGRQPFDYTMMYLNNIHDEGLVVLPTHRILSKEAVLGVNLQE
                     TLDDLAEHFEVEPLALNGGDVENEARRCVEQLSGAGASGTAFVMLLPKGRSYLLKLKP
                     QTDLDELIDDEKIARQIKSLDVTILHRYIINQAWLGNPEIELDDEDVRYEKDAAAVLK
                     AMSTCKWGVGFLLNPTRIEQVCEVARAGLRMPHKSTYFYPKLVTGLVMRDLNSPW"
     gene            complement(10940..12571)
                     /gene="lnt"
                     /locus_tag="PLS90_07590"
     CDS             complement(10940..12571)
                     /gene="lnt"
                     /locus_tag="PLS90_07590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPK02307.1"
                     /translation="MARRLRNALRDPGYWLAAAGGLCGVLAFDPYRLRPLIVLAPLLA
                     WLAVRAAATPGAAFRRLWLFGWVFTFGALKWLWTLFWVVGNPFIYPGVVLLGAYLGLY
                     YALAGWITRRWLWTPREWPGFVAFASLWLLSEWLRTLGRLAMPMPQVGHALAAWPWAI
                     QIASLLGEAGVSLGVLLTAGWLLSLLRLIQDRGGRGRCATRRAALTSTLLLLIFWSHN
                     VGSWLVWRGRVASWASAADARPLDVAMIQPNIDQISKLMSYYPWETPERRREAARLRE
                     VIAARNERLVEQDLTHRPDLIVMPETAYTELDFYSNERIRERVAALARRAGADIVFGA
                     GRDMSAAGRDEIYNSTYLVRADGSWDDTIYDKVRLVPFGETVPYFDIIPGLQESVVGI
                     GSFNAGQAMPLFETGGLQFGALICFESTFHQMARRYARQGADFLVVMTNDAWYLRSAG
                     APQHHNLSLLRAVETRRWLLRSANTGISSTITPAGEISRGLGLEMTGVVEDTLWFPPR
                     QGRTVFTRAGNAWLAMPFLFCAALFIWKMRAGKPA"
     gene            12972..13047
                     /locus_tag="PLS90_07595"
     tRNA            12972..13047
                     /locus_tag="PLS90_07595"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13005..13007,aa:Lys,seq:ttt)
     gene            13078..13153
                     /locus_tag="PLS90_07600"
     tRNA            13078..13153
                     /locus_tag="PLS90_07600"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13112..13114,aa:Glu,seq:ttc)
     gene            complement(13226..>13597)
                     /locus_tag="PLS90_07605"
     CDS             complement(13226..>13597)
                     /locus_tag="PLS90_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK02308.1"
                     /translation="LMKPDVMSTGAAIISCRDNDIIPSDTFIIDNDGAGLGVGNTLAD
                     AEYCIKTGTSMACPLAAGIAALLLEEAPGATPAQIKAILQNNAANAGSPDSNDGYGLI
                     DALATMQDAAVPVKFRQFIVE"
BASE COUNT         2260 a         4304 c         4794 g         2239 t
ORIGIN      
        1 tgcggctcat gcccggcttg cgcggcaggc cggcggtgac gacgaccacg tcggaaccgg
       61 cgatgtcctt gtaatcgttg gtgccctgga tccggctgtg gtagccgtgg atcgggccgg
      121 tctcatacat gtcgaggccc ttgccctggg gcatgccctc gacgatgtcg gtcaggacga
      181 cgtcgcccag gtgcttctcg gccaggaaaa gggccgtggt ggaaccgacg ttgccgccgc
      241 cgatgacgga gattttcttg cgcatgatgg gatacctgtt cttccgcgga tgatctcgat
      301 gggcgccgcc cgccgacgta aaaggaggga ataagcacgc ctcacatccg ttccgccaac
      361 cccgcccgca acgggatggg cggtcggact cccaacgtta gtgaagggcg ggaagggagt
      421 caagcagggg agaaggtcgg gaaaggaagc ggtggcaggg aacgcgggcg gcggaagcaa
      481 cgacggattt tacggaccga cggcaaggac gaggccgcgc gcgggtgcgg cgggcgctgg
      541 ccaatccccg gccgctcgcc tatcctgatc gcccatggac tccgcgaacc agtcccgccc
      601 cggcgcaggc cgccgcgcgc gcccgacacg tgccggccgg aggggcctgc cgctgctgct
      661 cctcctcgga gtcgcgctgc tgtggcggct gatgttcctg ctggccatcc acgacacgcc
      721 cgccggacag tggcaccagt gggaccaatc cgacatgcac acctacctga tgcaggggag
      781 gcaactggcc gccggcgact ggctggcgcg cgagccgtac tatccctacc acctgtggat
      841 gagcgcgggg agtcccgcgg cgtggcgggc gctctatccg ccgcatgttt ttcaccaggc
      901 gccactgctc agctacgggc tggcgctgtt tctgcgcttc ggcgtcgatc ccgtgccgtg
      961 gattcggctg ctgcacgcgg ttctgggctt gatcaacaca tatctgcttt accggctggg
     1021 ccggctcctg ggagggcgcg tcgcgggcct cgtcgccgga ttgttcggcg cgctgtatgg
     1081 tccgctgctc gtgatcgaga gccagcccct gcgcgaaccg gcggcgctgg cactgacgct
     1141 gctggcgctc ctgtgcgtgg cggcgatgcc aatccggagg aagggggggc gcgaacggac
     1201 gcgggcgctg gccttcggcg ccggattcgc gacgggggtg ctcgcgctgc tgcacgaagc
     1261 cgccgccgtc gtgctgctgt ccgccgcgct ggcgcttgcc tgggacggcc ttgtggtccg
     1321 ccgcgcgccg cgacgattcg cggcatgcct ggcgctgctg ctgggcggcg cgttgatcgg
     1381 cttcctgccg ttgctggcgc gcaatctggc cgtcggcgcg ccgcctttcg cgcaatcgac
     1441 ccgctcggcg cttacctggg cgatggccaa tcatcccgat gcgccgtggg gcggggttcg
     1501 ctggaccggc cccgacgcga gcttcttcga gacgctggag ggaacgcggg ggcgaccgtt
     1561 acgtgttgtg atccgcacgc tggcggccta cgagggcgcg ccgtggcgct gggcgctcaa
     1621 ctgggggcgc cgggcgctgg cgctgctcat cggcggcgaa gccagcgaca atacttcgca
     1681 cgcctggttc cgcctgcacg ttccgatcct ggcgctctcg ttggattttc gctggttgtt
     1741 cgcgctgggc gccgccgggc tgctgcggcg cgggcggcgc ggcgggtgcg gccggccggc
     1801 gccgatgctg gcgacctacg ggctgctgat gctcgccgcg ctgtcctcgg tcttcccgct
     1861 cggccgctac cgactgttcc tgctcccctg tctggcgccg ttcgcgggcc ggttggccgc
     1921 gcaagcctgg gccgcgcgcc ggaaggcgaa ctggcggcgg ctggggggaa tgggggcgct
     1981 cgtcctggcg gtgttgctcg gccagatggc gctggatcgg gcgtttgggg cgatcgccgc
     2041 gccgcgcccg gttgatttca gcgtcgccgc gcggatgctg accgaatggg gcaacccggc
     2101 cgccgccgcc gccgagcttg agctggccgc cgaacaaggc gtgctgaccc ccgcaatgaa
     2161 ggtggagctg tcgctggcgc acgcgcgcgc cgcgctgctg gcgggcgccg agcagcaggc
     2221 gctggcgagc ctggagtccg cgggccggtg gctcgcggag ctggacgagc cgccgccgaa
     2281 cgcctcggcg atcgcgaggg aatcagcctg gctgaagctg acggccgccg atgcgtcgct
     2341 gcgcgacccg gacggcgctg aacggattgt gcagggcctg gagttgcgcg aaaatggtcg
     2401 ggctgaactg ctggatctga aggccgcgct ggccgccgcg cgcggtcagt ttgacgttgc
     2461 tattgggctg gccaccgaag cccttgaagc ggctcgcggc ggcggcgacg aggttcaggc
     2521 gcgattcatc cagcgtcggc tcgagcgcta ccacgcggga cggccggcca cccggccctg
     2581 atttcaccga tcgtcggagc gactggcacg caattggcac gtaacgtagc tgaatgcgcc
     2641 tcgatccgaa aaggagaacg gcagtgaaac gcaattcagg ctggtatctg gtgatttctc
     2701 tggcagcgct ggcgacggcg gcgttgccgg cgccgacggc gtgggcgcag gagggcccct
     2761 cgcgcgtgct gggcgggccg cgctcggccc ccgccggtcc cggcggcgac acgatctata
     2821 tcgagcgcca cgacggccgc gcacgttcgg acggcgccgc ccggatcgaa cggcaagtcc
     2881 ccagcgcacg gcgctttcag gattcggaca tccggctgaa cgacctgcgg gatgccgacg
     2941 cgcgtcggct ccgcccgtac cgccgtttct atcgcgaccc gtatctccgc accgacgacg
     3001 agcggttcat cgaccaccgc ctggatccgc tgcgctaccg ttattacgat catttcgcct
     3061 atccctatcc ctacggcttc taccgctacg ggggcgagcc ggtcattatc atcgatgacg
     3121 cggtgattgc cggcgggacg gacacgcgcg gagggagcgt cacctaccag gccggggagc
     3181 ggacctattc ggcgggcggg gaggacggcc gacgctgggt ggagggccac tgggcggaaa
     3241 tgccgcgcga ggatctgatc gagggcgaaa cggtggaggt gttccacccg gccgtctatc
     3301 ggcagcgcga agacggctcg ctggaggaac ttgaggaaga gcgggtgacc cgggaaccga
     3361 agtaccgtgt ggtgctgacg ccggtgtgga tcgagggaca ctatgttgac gagcggggaa
     3421 tcccgctgga gccggtgcgg gtggaggaag cgccgacgga agaaccgtga gcgagggacg
     3481 atcctggccg gcgcttccag gcagcgtaag tcgaagttcc gaaggtttgc gcgtcgtgga
     3541 cgaatccgat aagatgtcgc tgccgtaaca cccgtcccct tgacggaaac gtgtggaccc
     3601 ccctccatcg gagccgttca gcttggatcc cgttcatatt ttttgttatt gctccacgac
     3661 cgttcccaat cgctttgccc atccgatcca gattctgaag acggtgcggg cactggcgga
     3721 acagccgggc gtacgcgtga cgctctattt cgtcagcctg ggcaccaagc cgcgcgcgat
     3781 ctggcgctac tacgggatga agccgctgcc caatgtgcgg gtccgcggca tggtgcccag
     3841 ctggtacttc cggctgatga agctgaacaa ccacctgtgc ccggagcagc agaagatctt
     3901 ccatctggtg cgctggcggc tcgcgcgcga actgcggcgc gtggcgcgaa ccgaacgggc
     3961 tttcatctat acacgcaacg agaatgccat cacgatgatg cgcaagagcg ccgacgccgc
     4021 cggcattccg atgtatctgg aactgcactg gttcaagcac ctggactggt tccgcaaact
     4081 gccctggaac cagtcgcgcg aggtcgctcc gccaccgatc ggggaataca agcggctgct
     4141 gcgcaagctg aaggcgaatg agcgcgaggt gctgaatcag gccgatggga tcctctgcct
     4201 gaccgacgag atccgcaagg tcttgtcgcg gtggaatctg aaatgcccgc tgacgacgct
     4261 gccgagcggt gtcgagctga tcgctcccga agagctgcgg gccaacggag tggaaccggc
     4321 cgagctcgag agcgatgact cggaagagga agtggcggcg gggggggacc tgggcggagc
     4381 cgacgacggc gacgattcgg cggacggcga tgccgcgcaa gatgaggcta tcgcgacgac
     4441 cgacacgtcc ccgccgaacc gaacgccgct cgacattctg tatctgggcc agctctaccc
     4501 ctggaaaggg gtcgacctgc tggtccaggc gatggcccac ctgccggagt accgcctgac
     4561 gatcgtcggc ggcaatcgcg acaaggacaa gaaccgggtc aaatcgctgg cgcgggaact
     4621 gggagtgaac ggccgggtca atctggtggg gcacatcagc caccccaagg tgaagcgcta
     4681 catccgcaag gcgcgggtgt gcgtggtgcc gttgccgcgg gccggatacc gcgaggcgcg
     4741 gttcttcacc agcccgatga agctgttcga attcgcggcg tggggcaagg tgatcgtcgc
     4801 cagcgatctg ccgaccttgc gcgaagtgct gacgcacggg cagaatgcgt tgctcgtgcg
     4861 gccggacgat ccggtcgcgc tggcggaggg actgcgcgag gcgctggaga accagccgct
     4921 gcgcgagcag ctggcggccg gcgcattgaa gttggcccag gaccacgcct atgaaaatcg
     4981 cgcgcggcgc atcctggagt tttgccgccc gggcgggaaa gtgacggcat gaactggaaa
     5041 gagcgggttc agcagtgggt cgtcgagcca acgctgcgag gactgggcgt gccggtgctg
     5101 cggcaatacg gctttgaacc gaccgaaccg ccgcgtgttc tcctttacac ggcgagcaac
     5161 gtcggcctgg ggcatctgtt ccgcgtcctg cgcacggcgg ccgagctgcg ccggctgctg
     5221 ccgcgcgtca acctgctgct gctgaccgac accgagcacc tgcacgtgac gcagtattat
     5281 gggcagttgg cggtgctgcg gctgcccaat ttccattatt tcggcgagaa ttttcgtgaa
     5341 aaaccggtcg gcctggagct gagcaagggc aagctgcgcc tgctgcgcta caatgcgatg
     5401 ctggcggcgg tgcacagctt tcagccgcac gtctttctga tggacacggc gccgcacggc
     5461 aagcgcaacg agctgtatcc ggtgctggag tatctggccg cgcagcggcg gccgccgatc
     5521 cgcatcctgc agttgcgcga cgtaccgttc gtgccgggcg aagccgagcg cacggacgac
     5581 gcccgccgcc aactgacgcg caacccggat ttctacgact acctgttcgt ggcaggggac
     5641 ccgcagtact tcgacctggc caaagtctac gactggccga agaagatcac gcgcaagttg
     5701 ttttacctgg ggttcatcat ccccgaggcc gacggagatg gcccgccggc agtcgaatcg
     5761 ggaaccgaac gcgaaatcga gcggatgatg cagcggccgg cgcggcggat tgtcgccagc
     5821 ttcggcggcg gctgggaagc ggagggactg ggcggggcgc tgctggcggc ctatgccgaa
     5881 ctggccgcgc agcgcggcga accgttgcag ttctatctgt tcaccggacc gtcggccgag
     5941 gatgccgcgc tggccgggct gcaggcgcgc gcggcggggc gcgacgacgt gctgatccgc
     6001 aagttttcac cgcgcttttc gcacctgctg gcgcgttgcg acctggccgt gctgcaggcg
     6061 ggctccaccc cgtttcagat tctcgagacc gacatcccga tgctgctcta cgcgcgcgat
     6121 ttcaagagca aggagcagca gttccgggcc gagcagatga tccggttcgc caacgtggag
     6181 ctgctgggcg aggatggcgg gcagccggcg gttctgcgcg cggcgatggc gcggctgctc
     6241 gatgctccgc gcgtcccgcg ccggacgggg tttcgctacg gcggcgtgcg ccgctcggcg
     6301 cgggcgatcg cggcgatgct ggcggacagc cgcaaacccc ggcggctcag cgtcgaggag
     6361 ctcaaccgga tctgccgcga ggaatgaggg tgaattcccg tttgatgcgt aaataggaat
     6421 caggccgcga agaaccaccg tcggttgagc cgtttgaagc cggtcgtcga gggttctcgt
     6481 cggcctttgc ccttcaaaat cgcgcgttat tccgtttcaa tggtgggttt ccgtgggacg
     6541 gggcgggtta attaccagcc cttgccgcgc aaatgggggc gggcttcgga ggcgaggacc
     6601 aggacgagtt gctcattaac cgggggttca ccgaggggac gaatttgggc agaccttcgg
     6661 gagcgatcac gaggacgaga gacgagagac gattttagag tgagggacga attctggagg
     6721 gcggtcatgg cgatcgagac gttttatccg ttcgaggaag tggaacccag atggcagcgg
     6781 gcgtgggacg agcagggcct gtggaagacg cccgccggcg ccgcgcggcg caaatactac
     6841 gttctggagc agttccccta ccccagcggc aagctgcaca tggggcatgt gcgggtctac
     6901 acgatcggcg acgcgatcgc gcgcctgcgc cggatgcagg gctacgacgt gctgcacccg
     6961 atgggctggg actcgttcgg cctgccggct gagaatgcgg cgatcaagaa cgacgcgcag
     7021 ccggcggcct ggaccgagca gtgcatcgcc aacatgcgcg agcagttcca ccggctgggc
     7081 atttcgtacg actgggagcg ggagatttcg acctgccgcg acgactacta ccgctggaac
     7141 cagtggatat tcatcaggat gtgggagcag gggctcgtcg agcggcgcaa ggcggcggtc
     7201 aactggtgcc cgcggtgcgc gacggtgctg gccaacgagc aggtgatcaa cggccgctgc
     7261 tggcggcacg aagacaccga ggtggagatc cgcccgctgg agcagtggtt cctgaaaatc
     7321 acggactatg ccgaggaact gctgaaggat ctggaggaga agctcggcgc gtggccaaac
     7381 ctggtcaccg cgcagcagcg caactggatc ggccgcagcg agggggcgct ggtcaagttc
     7441 accgtcaagg agacgggcga ggaattgccg gtgttcacga cgcgccccga cacgctgttc
     7501 ggcgtcacgt tcatgtcggt cgcggcggag cacccccggg tgcgcgagtg gatccggggg
     7561 cgcgagaacg aacgcgaggt gcaggccttc atcaatcacg tcgtgatgga ggggcgcgcc
     7621 gcgcggacag acgaggccgc cgagaaaaag gggatcgcgc tgggcatcca tgcgatcaac
     7681 ccggtcaacg agcgcgagat cccgatctac atcgccaact tcgtgctgat ggagtacggc
     7741 acgggcgcgg tgatggccgt gccggcgcac gaccagcgcg acttcgagtt cgcccgcaaa
     7801 tacggcattc cggtcgaggt cgtgattcag cccgaaggca agacgctgga cgcggcgtgc
     7861 atggaagcgg cgtatgtcga accgggggtg atgacccatt cggcgcagtt cgacgggctc
     7921 ccctcggagc aggccaaaca ggagattacc gagtggctgg cgcgccaggg caagggcgaa
     7981 aagacggtgc agtaccggct gcgcgactgg ctgatctcgc gccagcgctt ctgggggaca
     8041 ccgattccgt tcatctactg cgagggctgc ggcctggtgc cggtcccgct ggaggacctg
     8101 ccggtcaagc tgccggagaa cgcgcggttc gggggggaag gcaatccgct cgccaccgtg
     8161 ccggagtggc tcgaggtgga atgcgagcgc tgcggcggca agggccggcg cgaaaccgac
     8221 acgatggaca cgttcttcga cagctcgtgg tattacctgc gcttcaccga tccgaagaac
     8281 gagcgggttc cattcgacaa ggcggccgcc gagagctgga tgccggtcga tatttacgtg
     8341 gggggcaagg agcacgcgat tctgcacctg ctctactcgc gcttcttcac gaaggtcctg
     8401 cgcgacatgg ggctggtgac gctcgatgag ccgttcaagc agctcctgac gcaggggatg
     8461 gtgctcaaca cgggaaccga cccgcggacg ggcgagacga agatgttcaa aatgagcaag
     8521 tcgatcggca acgtgatcga tcccaaccag ctcatcgccg agcgcggcgc cgacgtggcg
     8581 cggctgttca tcctgttcgc ttcgccgccg gagaaggacc tgccgtggtc cgagcagggg
     8641 gtggagggga tgtcgcggtt cgtcaaccgg atctggcgcc acgcgcagaa ccacggcgag
     8701 gcactgcgcg cggcgggcgg gggggacgta acgcccggcg aaattacgga ggaacccgac
     8761 agagcgctgc tgcggatgat ccacgaggcg acgcggcgcg tcacgcacga tgtcggcgag
     8821 cgcttccagt tcaataccgc gatctcggcc tgcatggaac tgttcaatgc catcggcgat
     8881 tacgcgccgc gcgcaggcga tgcgctgagc gcgcgcctgt gggcgatggc gacgcgccgg
     8941 ctggcggtgc tgctgacgcc gttcgcgccg cacctggccg aagagctgtg gcagcgggtc
     9001 ggcggcgagg ggctggtggc ccagcaggcg tggccgtcgt gggatgaggc cgcgctgcgc
     9061 gtcgagacga tcgagatcgc cgtgcaggtc aacggcaagg tgcgcggcaa ggtgcgggta
     9121 gcggccgagg ccggcgagga ggcggtgcgc gcggcggccc tggccgacga aaccgttcag
     9181 cgtttcattg acggcaagac catccataaa ttcgtgtatg tgaagggcaa gctggccagc
     9241 ctggtcgtca agtgaaggga ggagagacga agtagcgcgc taccaggggg agttcagatc
     9301 gcgcatgacc aggccggtca ccagtttggg gtagaaatac gttgatttgt gcggcatgcg
     9361 caggccggcc cgcgccactt cgcacacctg ctcgatccgc gtcggattga gcaggaaacc
     9421 gacgccccac ttgcacgtcg acatcgcctt gaggacggcg gccgcatcct tctcgtagcg
     9481 cacgtcctca tcatccagtt cgatctcggg gttgcccagc cacgcctgat tgatgatgta
     9541 gcggtgcagg atcgtcacgt ccaggctctt gatctggcgg gcgatcttct cgtcgtcgat
     9601 cagttcgtcc agatcggtct gtggcttcag cttgagcagg tagctgcgcc ccttgggcag
     9661 cagcatgacg aaggcggtcc cgctcgcgcc ggcgcccgag agctgttcca cgcagcggcg
     9721 cgcttcattc tccacgtcgc cgccgttcaa cgccagcggt tcgacttcga aatgctccgc
     9781 cagatcgtcg agcgtttcct gaaggttgac gccgagaacc gcctccttgc tcaggatgcg
     9841 atgcgtcggc aggacgacca gcccctcgtc gtggatgttg ttgagataca tcattgtgta
     9901 gtcgaacggc tggcggccgt cgcgccgtcc ggtgatctcg cgcatctcgt cgcggaagag
     9961 caggctggtc tcatagcgat gatggccgtc ggcgatgaac agcgtctggt ccttcatccg
    10021 ttcggtgatg cgcgccacga ccgagctctt gcacagcggc cagaggcgct ggacgacgcc
    10081 atcgaatcgg gtttcgatcg ccttgctcgc cgtcgcctgg gccagcagtt catccacctg
    10141 gcggctcggg tcgtcgtaga ggcagaagat cggactgagg ttggcattgg tcgcgcgcat
    10201 cagccggaag cgatcggcct tgggtccgtc gaacgtgtgt tcgtgggcgc ggattccgcc
    10261 ctcgctgaag tcctgcaggc ggacggccgc atagaacccg cggcggcgca cggtgcggcc
    10321 gtcgggcagc tcaaattcct gctcgtagac gtggaagcat ttcttcgggt cctcgacgac
    10381 gaccttttcg tcgcgccact ggtgccagag ggcgccggcg cgcgcgtagc gattctcgaa
    10441 ctcattgtcg tcgggcatcg tcttgccgaa atcgatgcgc acaatgttgt gcgggtcggc
    10501 gtcatagaat gcctgttgca tcgcgggggt gatcacgtcg tacggcggcg tcagcacgcg
    10561 cgccagttcc tggctgcgcg tcagcgcgta tcgcgtggct acaaagggag caatgtcagc
    10621 catgctgttg tttcctcggc ggcttcctgc ccgatttcaa tatctgcctg gaccgccggg
    10681 cgcgacggct cgagcatgca gttcagtaga atgtgatcgg tatcattccg agcgaacatc
    10741 cggggagagc gcacgacccc tctcccaaag ttccacgccc gcccgtctca agtcaaccct
    10801 gttcctatcg cggcgcatct tccccccgat accttcatcc tggccccgca gggcggcctg
    10861 cgcaggcgcc ccggccgatc cgccggcatc cgccccgggc aatggcctgc cgtgtgtggg
    10921 gaacatcaca ccgcggaatt cacgctggct tgccagccct cattttccat ataaacagag
    10981 cggcgcaaaa caaaaacggc atcgccagcc aggcatttcc cgcgcgggtg aagaccgtgc
    11041 ggccctggcg cggcgggaac cagagcgtat cctcgaccac gccggtcatt tccagcccca
    11101 ggccgcgcga gatttcgccc gccggcgtga tcgtggagct gatgccggtg ttggccgagc
    11161 gcagcagcca gcggcgcgtc tcgaccgcgc gcagcaggga gaggttgtga tgctgcggcg
    11221 cgcccgcgct gcgcagatac cacgcgtcgt tcgtcatcac caccaggaag tcggccccct
    11281 gccgcgcata gcggcgcgcc atctggtgaa aggtcgattc aaaacagatc agcgcgccga
    11341 actggagtcc accggtttcg aacagcggca tcgcctgccc ggcgttgaac gagccgatcc
    11401 cgacgacgct ctcctgcaga ccggggatga tgtcgaaata cggcacggtc tcgccgaacg
    11461 gcaccagccg caccttgtcg tagatcgtgt cgtcccacga tccgtcggcg cgcaccaggt
    11521 aggtgctgtt atagatttcg tcgcggcccg cggcgctcat gtcgcggccc gcgccgaaga
    11581 cgatgtcggc ccctgcccgg cgcgccagcg ccgccacccg ctcgcggatc cgctcgttgc
    11641 tgtaaaagtc cagttcggtg taggccgtct cgggcatgac gatcaggtcg ggccggtgcg
    11701 tcaaatcctg ctcgaccagc cgctcgttgc gcgcggcgat cacctcgcgc agccgcgccg
    11761 cttcgcgccg ccgttccggc gtctcccacg gatagtagct catcagcttg gagatctggt
    11821 cgatgttcgg ttgaatcatc gcgacatcga gcgggcgcgc gtcggcggcg gaagcccagg
    11881 acgccacgcg cccgcgccag acgagccagg aaccgacgtt gtgcgaccag aaaatcaaga
    11941 ggaggagcgt ggaggtcagc gccgcgcgcc gcgtggcaca gcggccgcgc ccgccgcgat
    12001 cctggatcag gcgcagcagc gacagtagcc accccgcggt tagcagcacg ccgaggctca
    12061 cgccggcttc gcccagcagg ctggcgatct gaattgccca gggccacgcc gccagcgcgt
    12121 gacccacctg cggcatcggc atcgcgagcc gccccagcgt ccgcagccat tcgctcagga
    12181 gccacagcga ggcaaacgca acgaagccgg gccattcgcg cggcgtccac agccagcgcc
    12241 gcgtgatcca gcccgccaga gcgtagtaga ggccgagata cgcccccagg agcacgacgc
    12301 cggggtagat gaacggattg ccgaccaccc agaacagcgt ccagagccac ttgagcgctc
    12361 cgaacgtgaa gacccagccg aagagccaca accgacggaa cgcggcgccg ggcgtggccg
    12421 ccgcgcgcac ggccagccac gccagcagcg gcgccagcac gatcagcggc cgcaggcgat
    12481 acggatcgaa ggcgagcacg ccgcagaggc cgcccgccgc cgccagccag taaccggggt
    12541 cgcgcagggc gtttcgcaat cgacgcgcca tgcggcggat gcgtccggct ggggcgtccg
    12601 ccggcgtggg cgggcgttcg gcctggcggc gatccggaac gggatctcgc gcgggatgac
    12661 gcgccatacg ggtggtgcct tcgttggggt tggatggggc gcggcggatg cggggcgcgg
    12721 cggaacggct ctccgcccgc cagtctatgc aagccgaccg gggtcgccta tgaaaaaccg
    12781 aacttccaca atgtgttggg tcagcgagac gccgatttgc tccggtgatt cacgcgcaac
    12841 cgccgcgatc gcggcgtttt ccccgtttga cttgggcccg gtcgcccgct agactgttgt
    12901 gctttggaga cgtccggagg ggcaccggtg tgtgccgccg ggcggattcg agccacggta
    12961 aatcgggtcg cgagcgcgta gctcagtcgg cagagcagcg gccttttaag ccgttggtcg
    13021 tgagttcgaa tctcaccgcg ctcaccaccg tctcccccgt ggccggcgct ccaacgcgtc
    13081 cccatcgtct agaggcctag gacaccggat tttcattccg gcgacagggg ttcgactccc
    13141 cttggggacg ccaaccgttt acccccccgg cgccatgagc gcacgggggg gttcgctttg
    13201 gtagggaacc gaatgatccc gagaactact ccacgatgaa ctggcggaac ttgaccggca
    13261 cggcggcatc ctgcattgtg gccagcgcat cgatcaggcc gtagccgtcg ttgctgtcgg
    13321 gcgaaccggc attggcggca ttgttctgca gaatggcctt gatctgggcg ggcgtcgcgc
    13381 ctggggcctc ctccagcagc agcgccgcga tgccggcggc cagcggacag gccatcgacg
    13441 tgccggtttt gatgcaatac tccgcatcgg cgagcgtatt gccgacgccc aaccccgccc
    13501 catcgttgtc gatgatgaac gtatcggagg gaatgatgtc attgtcgcgg caggagatga
    13561 tcgccgcgcc ggtggacatc acatcgggct tcatcag
//
LOCUS       DAOPCO010000082        13491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4571_length_13491_cov_4.804034, whole genome shotgun sequence.
ACCESSION   DAOPCO010000082 DAOPCO010000000
VERSION     DAOPCO010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13491
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4571_length_13491_cov_4.804034"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PLS90_07610"
     CDS             complement(<1..1112)
                     /locus_tag="PLS90_07610"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPK02309.1"
                     /translation="MTDRTPDDPTGMIPLGLLACPVTVRRPQRTRIIAGLRPELRRLK
                     DNPREPHAIEVSSGLREPLGWLPRATSAWLAPLIDAGRVALRPLALRVASTSAGEAEY
                     AIDIEVFLTRQGAEILHTRGEGGGAPHVLHRMLVRLWRRCERARHNQRMGQDIAIVLA
                     RLDPRQLLPESNLLLNLLPTLHDYRRRLDEREQALGDARRRLGAVQFGEPVRHGSLAL
                     IPLLGSNGHVPSYELLHEALSAGKARVDELNPGGVVPFLKIVNESTQPLLVCEGMLLI
                     APKQNRVVNESLLVPNEMEFALPVSCVEQGRWHRSGRAAEVRGGATALLRSRKLRTLL
                     RRRDAGYANPAQGEVWDEVQACLREMDAPSPTHDLDA"
     gene            1303..2853
                     /locus_tag="PLS90_07615"
     CDS             1303..2853
                     /locus_tag="PLS90_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK02310.1"
                     /translation="MPGRVILALSPLPSLLVQAARCHGLWREIRVVGRKHVHPFMDEC
                     LQAGEPAELLLLLREVDAPTELGRRFAAFGRLGGLIQVFAPRDSPSVRAACRGLEHVR
                     LHTASSVEEAFERVYPGHNLYGDLSNPDLRDYLQYKITICFMRLMDPQPLEEAAQALY
                     ANGERIFSLARAVPEEAGSVEHFREADFPYIEGQSRPVLELKRRIREVGASSLNVLLL
                     GETGTGKEFAAFYLHEFSPRRHGPFVAINCAGLNETFLRSELFGHLKGSFTGAVAENR
                     GLVHQAEGGTLFLDELAEMPLPVQADLLRFLQSRRYRPLGAAKERKADVRIIAGTQPV
                     LREKLRRGEFREDLYYRLAEVEIPTPPLAAIPEDIPRIIRHVCYRMADRMRDGADPRD
                     VIQYFRQGERTLKKYPWPGNVRELISLVRRRVELGDDVVAELKERMERKLSEANPAVA
                     VEGVGAASAPPGFSRIEPLKTIVARYVQWAHARRGALSQQDLARRLGISVNTLKKHLA
                     AAARGVVN"
     gene            3013..3870
                     /locus_tag="PLS90_07620"
     CDS             3013..3870
                     /locus_tag="PLS90_07620"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPK02311.1"
                     /translation="MTARTRLLGVMGWPVEHSLSPPMQNAAIEALGLDWRYVALPVRP
                     EDLAEALRGARAMGFVGVNLTIPHKQQAMRLLDRVDPLAAAIGAVNTVHFTAQGARGY
                     NTDADGYVRTVEQESGYSFRGGRVLQVGAGGVGRAMAFGAARAGAASLAIVNRDPARA
                     RALAADLAASFPGLRIQVVESAAERAAAAAGADLIANATSLGMKPGDPVCLPPELIRE
                     QHVVFDTVYTPDPQTPLLRAARERGARCVEGLGMLARQGAAALRIWSGREPDEALMER
                     VLRRHLGAE"
     gene            3979..5532
                     /gene="lysS"
                     /locus_tag="PLS90_07625"
     CDS             3979..5532
                     /gene="lysS"
                     /locus_tag="PLS90_07625"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPK02312.1"
                     /translation="MAESERIEPSPASETAAADEHRLVRERYDKAARLRATGWDLYVK
                     KHEPTHHAADLRARQQELVASGEPVSIAGRVMSVRAFGKAGFLLIRDETDQIQAYVKK
                     DVTDERGYDYFRHGLDAGDIVGASGKLFYSKTGELTVEAAQLRLLTKAIRPLPEKWHG
                     LKDVELRYRQRYVDLLANPDVRETFRVRSRTIAALRRFLDERGYMEVETPMMQPIYGG
                     AAARPFITHHNTLDMDLYLRIAPELYLKRLLVGGLERVYEINRNFRNEGLSTRHNPEF
                     TMLELYTAWWDYTDTMDLTETLIRETARSVLGAAELAYQGHRLDLGQPFRRVRILDAV
                     AELLGLAAGDANPLRWGLGDMERLRAALGGAGDEIAGILREESDPDEALMRVFEERIE
                     PELIEPTFVIDYPKSKCPLAKSSPADPHTAERFELFIGALETANAYSELNDPREQLEQ
                     FECQVRQRAGGDEEAMNEIDRDYIRALEYGMPPASGLGIGIDRLIMLLTDSASIRDVI
                     LFPLMRPER"
     gene            5597..6814
                     /locus_tag="PLS90_07630"
     CDS             5597..6814
                     /locus_tag="PLS90_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02313.1"
                     /translation="MKSLEWFIAWRYLVSRERKALVSLITFISMTGVAVGVAALITVI
                     GVMDGADELLFGKVAELYPHLRVVNLEGPREEFDPEVIRKLKALPGVTHVQPVMQKQA
                     FIIAGQGRDQNRQSFVQLLGMDRLDQNTIYSIMTYEHGDSVTLGEDEILLGLPLAVNI
                     GAMINDRVTLVSTAPVRTAIGPQMRFLKFTMKDGFRTDFYEFDANTAFISTEMMRKLF
                     PTEPGYDYLHVKLKDPFQADRLAARLHLPGYRISTWSQENGAFFSALKLEKLALFVIL
                     LLIIFVAALNIIGTLILMVNDKTREIGILKAIGASRRTIWKIFLLDGVMIGILGTGAG
                     VAVGLLLCFSIPYIPLDLPASVYNFSRLPVEVQPVTVLLIVCSSMVICTLAALFPAIQ
                     AARLHPVEALRYE"
     gene            6807..7535
                     /locus_tag="PLS90_07635"
     CDS             6807..7535
                     /locus_tag="PLS90_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017113577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02314.1"
                     /translation="MNSLLTARDIYKSYHDGERELRVLRGASLSVARGEAVAVVGMSG
                     AGKSTLLHLLGGLDVPDRGEIAVDGETISGRSLTFLHEFRNRKIGYVFQFHHLLPEFT
                     ALENVMMPALIAGGQRKTVAGEARQMLERLGLADRERHRPSKLSGGEQQRIALARALM
                     NQPALLLADEPTGNLDLHTGDVVINLMWEMTVKQGRGLVIVTHEPLIAARADRVLRLH
                     EGTLLEVARNEIEAQMAQGPGAKP"
     gene            complement(7830..9008)
                     /locus_tag="PLS90_07640"
     CDS             complement(7830..9008)
                     /locus_tag="PLS90_07640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02315.1"
                     /translation="MRIAYLCADPGIPLYGRKGCSTHVRETARILMSLGHSVRIWCTN
                     VGGDETDGPPLDVVHVRPVQSKKLGFDLRHILTDQRLWRRIRADFAQWRPDVIYERYS
                     LYARTGTRATAEFGLPRLLEVNAFLTKEQHQRIRLAWLARRVEGDIIRRAPRVIVVSE
                     PLRREVHELGQSMAAIAKMPMAVNLDKFHPDGPGREELRRRHGLHDRFVIGYVGTLAG
                     WHGIRLLYDVARRLRKRATTGYAFLIVGGDGLKLEMHRRKAREAGLEDVLRFVGSVPH
                     DDVPAHIRAMDAAIVPDTTYWSSPAKLFEYQACGVPVLAPAYPAILESLEDGREGLIY
                     PAGNVKQIAKAVLRLMADPALAGTMGDAARRRAELEHSWLHNGEQILTLYHELGARVT
                     "
     gene            complement(9038..9811)
                     /locus_tag="PLS90_07645"
     CDS             complement(9038..9811)
                     /locus_tag="PLS90_07645"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPK02316.1"
                     /translation="MARLCVNIDHVATLRQARRGIEPEPVFAVGEVELGGSSGVTVHL
                     REDQRHIQERDVRLIREVVQGHLNLEMAAAESVVEKALRIRPDIATLVPERREEVTTE
                     GGLDVRGNLESLRKVTERLRDAGIRVSMFIDPEGDQIDASLACGADEVELHTGQYALA
                     RKPEARLAELRKLYHAARQVHAAGLVLNAGHGLNYSNVQAVAAIPHLQELNIGHAIVS
                     RAVFVGLRSATAEMLRLIDIASRSPEAARPAGLGPVGHS"
     gene            complement(9836..10945)
                     /locus_tag="PLS90_07650"
     CDS             complement(9836..10945)
                     /locus_tag="PLS90_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02317.1"
                     /translation="MEASRARSEFVLIGMALFIAFVIWLIAKQGELEIDRLEIPVQLE
                     NEGQIMNVRISPQFVRIAVQYPVDLRNSIVPSNFSLRINVREVFREDPATWKTPEQLD
                     VEYSLSEDMVRRPSLSQRVQVIDIEPDRVTLDAELVTRRVRVVPYTVGELPPTLRLVG
                     EIRATPPELLVTGTTDGFNALALQQNQILTEPIDLRKITQSNHIFANLVIPEGVKILQ
                     REEERVAIDIAVEEVSEQRTLNDVAINVPIAAENIAARVRPPTANVVVEGPPSALNRL
                     TQEDILLSPQRGLEERVGNVQKVGLEARFRQSVPPDVIQRVKIIETRPTSIEVEFVTP
                     SGEDEAGAEPELPVATESAPLRPGAGLPDGARDGQ"
     gene            complement(10945..11751)
                     /gene="cdaA"
                     /locus_tag="PLS90_07655"
     CDS             complement(10945..11751)
                     /gene="cdaA"
                     /locus_tag="PLS90_07655"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPK02318.1"
                     /translation="MQELTTSIMNFRLLDLADILLVALFFYGIFAILRETRSFVALMG
                     FVTFTLASLLLFLIALTWDLQAMILIFQNFWIVVVLIFLIVFQNEFRKALTDLGQLRL
                     LRGLFPSRERDVLDEIINAVTLLAGRNIGALIVLERRNPLRSYMGSGTVLDATVSAEM
                     IRTVFTPYSPLHDGAMLIRGERILAAGCILPLSDNPELSSELGTRHRAALGLSEETDA
                     VVIVVSEETGTISLAENGQIQRNLKPAELRSRLEEELEIAHQQAQGEEAR"
     gene            12373..12834
                     /gene="rplM"
                     /locus_tag="PLS90_07660"
     CDS             12373..12834
                     /gene="rplM"
                     /locus_tag="PLS90_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPK02319.1"
                     /translation="MGYTKSAKAEALPEKWYVVDARDQVLGRLASDVAHVLRGKHLTN
                     YTPHANMRTHVIVLNADKIRLTGDKWETKLYHHHTGWVGHLRETNARKLNERKPGELV
                     RKAVWGMLPKNRLGHATMTRLRIFSGDQHPHQAQKPEPLPTRQAAAQSAAR"
     gene            12882..13271
                     /gene="rpsI"
                     /locus_tag="PLS90_07665"
     CDS             12882..13271
                     /gene="rpsI"
                     /locus_tag="PLS90_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006689594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPK02320.1"
                     /translation="MEQYLGTGRRKTSVARVYLRPGRGRLIINGLAPEVYFSKRPMVE
                     DVVRAPLRETHTVSKYDVVVNVFGGGVKGQAEAIRHGIARALQNANPAYRPTLKALGF
                     LTRDAREKERKKYGLHKARKATQFSKR"
BASE COUNT         2282 a         4601 c         4317 g         2291 t
ORIGIN      
        1 gcgtccaggt catgggttgg cgacggcgcg tccatctcgc gcaggcaggc ctgcacctca
       61 tcccagacct cgccctgcgc cgggttcgcg tagccggcgt cgcggcggcg caatagcgtc
      121 cgcagcttgc gtgagcgcag cagggccgtg gcgccgcccc gaacttcggc ggcgcggccg
      181 ctgcgatgcc accgcccctg ctcgacgcaa ctgaccggca gggcgaactc catctcgttg
      241 ggcaccagca aggattcatt cacgacgcga ttctgcttgg gcgcgatcaa cagcatcccc
      301 tcgcatacca gaagcggctg ggtcgactcg ttgacgatct tcaggaacgg gacgacaccc
      361 cccggattca gttcatcgac ccgcgccttc cccgcggaca gggcctcgtg caacagttcg
      421 taggacggga catggccgtt gctccccagc agcgggatca gcgccagcga accatggcgg
      481 accggctcgc cgaactggac cgccccgagc cgccgccgcg cgtcgcccag cgcctgttcg
      541 cgctcgtcca gccggcgccg gtagtcgtgc agtgtcggca gcaggttgag cagcaggttg
      601 gactcgggca gcagctggcg cggatcgagc cgggccagca cgatggcgat gtcctgcccc
      661 atccgctggt tgtgtcgggc ccgttcgcat cggcgccaga gccgcaccag catccgatgc
      721 aggacgtgcg gcgcgccgcc gccttccccc cgggtgtgga ggatctccgc cccctggcgc
      781 gtgagaaaga cttcgatgtc gatcgcgtat tccgcttcgc cggccgaagt cgatgcaacc
      841 cgcagcgcca acggccgcag cgccacccgg ccggcgtcga tcagcggcgc cagccacgcg
      901 gacgtcgcgc gcggaagcca gccgagcggt tcacgcagtc cactggagac ctcgatggcg
      961 tggggctcgc gcggattgtc cttcagccgc ctcaattccg ggcgcaggcc ggcgatgatc
     1021 cgcgtccgtt ggggccgcct caccgtgacg gggcaggcga gcagccccag cgggatcatc
     1081 ccggtcggat cgtcgggtgt ccgatctgtc atttcttcgc tccttcctcc ggtgggtttg
     1141 aatgacttgc cgcggaggca ttaaggcgaa ttcggggcca acggcgacgg tttccatgga
     1201 agcttgaatt acctttattt aggctgatga agcgatgctg cttgaaccaa acgtgactct
     1261 cagataataa tgatccatca atttatgacg gaggatgaac ccatgccggg tcgggtgatc
     1321 ctcgcgctgt ccccgctgcc gtcgctgctg gttcaggccg ctcgctgcca cggcctgtgg
     1381 cgcgagatcc gtgtcgtcgg acgcaagcat gtccatccgt tcatggacga gtgccttcaa
     1441 gcgggtgaac cggcggaact gctgttgctg ctgcgggagg tcgatgcgcc gaccgaattg
     1501 ggccgtcgct ttgccgcctt cggccgcctt ggcggattga ttcaggtgtt tgcgccgcgg
     1561 gattcaccca gcgtccgcgc ggcctgtcgc ggattggagc atgtgaggct ccacacggcc
     1621 tcctctgtcg aggaagcgtt cgaacgcgtc tatccagggc ataatctcta tggcgacctg
     1681 agcaatcccg acctgcgcga ctatctccag tacaagatca cgatctgctt catgcggctg
     1741 atggaccccc aaccgctgga ggaagccgcg caggcgctct acgccaacgg cgagcggatc
     1801 ttttcgctgg cccgcgccgt ccccgaggaa gcagggtcgg tcgagcattt ccgtgaagcc
     1861 gacttccctt acatcgaggg gcagtcccgg ccggtgctgg aattgaagcg gcgcattcgc
     1921 gaggtcggtg cctccagttt gaacgtcctg ctcctgggcg agaccggcac cggcaaggaa
     1981 ttcgccgcct tctatctgca tgaattcagc ccccgccgcc acgggccatt cgtcgccatc
     2041 aactgcgccg ggctgaacga gaccttcctg cgttcggaac tgttcgggca cctgaagggt
     2101 tcattcacgg gcgccgtcgc cgaaaacagg gggctggtgc atcaggccga agggggaacg
     2161 ctctttctcg atgaactggc cgagatgccc ctgccggtgc aggccgacct gctgcggttc
     2221 ctgcaatcgc ggcgctatcg gccgctcggc gccgccaagg aacggaaggc cgacgtgcgc
     2281 atcatcgccg gcacgcagcc ggtgttgcgc gagaaactgc gccgcggcga gttccgcgag
     2341 gatctctact accgtctggc cgaggtcgag atccccacgc cgccgctggc ggccatcccc
     2401 gaggatatcc cccgcatcat ccggcatgtc tgctaccgca tggccgaccg gatgcgcgac
     2461 ggcgccgatc cgcgcgatgt gatccagtac ttccgccagg gcgaacggac gctcaagaaa
     2521 tacccatggc cgggcaacgt ccgcgaactc atcagcctcg tccggcgccg cgtcgagctg
     2581 ggcgacgacg tcgtggccga actgaaggaa cgcatggaac gcaaactgtc cgaggcgaat
     2641 ccggccgtcg ccgttgaggg agtcggcgcc gcctccgcgc ctcccggatt cagccggatt
     2701 gaacccttga aaacgatcgt cgcccgttac gtccaatggg cccacgcccg ccgcggcgca
     2761 ctgagccagc aggatctcgc gcgccgcctt gggatttcgg tcaacacact gaagaagcac
     2821 ctcgcagccg ccgcaagggg cgtggtgaat tgatcaaggg ctcctggatc ggcgcgcctc
     2881 cgccgccatg gccctttcgt cgctctttcc gtgtgtccct gattcctttt cccgtcccgg
     2941 tttccgatga cagaaccccg cgtgtttggc aggatggggt ccatccgcgc gtcggagggc
     3001 aacgtggacg acatgaccgc caggacgcgt ctgctcggcg tgatgggctg gccggtggaa
     3061 cacagcctgt cgccgccgat gcagaatgcc gccatcgaag cgctggggct ggactggcgc
     3121 tacgtggcgc tgcccgtgcg gcccgaggat ctggccgagg cgctgcgcgg cgcccgcgcg
     3181 atgggattcg tcggcgtcaa tctgacgatc ccccacaagc agcaggccat gcgcctgttg
     3241 gaccgcgtgg acccgctggc ggcggcgatc ggcgcggtca acacggttca tttcacggcg
     3301 cagggcgccc gcggctacaa caccgacgcc gacggctacg tccgcaccgt cgagcaggag
     3361 agcggatact cgtttcgcgg cgggcgcgtg ctgcaggtcg gggccggcgg cgtgggacgg
     3421 gcgatggcgt tcggcgccgc ccgggccggc gcggccagcc tggccatcgt gaatcgagat
     3481 ccggcgcgcg cccgcgcgct ggcggcggac ctggccgcga gctttccggg gctgaggatc
     3541 caggtcgtgg aatccgcggc ggaacgggcc gccgccgccg ccggcgccga tctgatcgcc
     3601 aacgccacct cgctgggcat gaagccgggc gatccggtct gtctgccgcc cgaactgatc
     3661 cgggagcagc acgtcgtttt cgacacggtt tacacgcccg acccgcaaac gccgctgctg
     3721 cgcgccgcgc gcgagcgggg agcgcgctgc gtcgaggggc tcgggatgct ggcgcgccag
     3781 ggagccgcgg cgctgcgcat ctggtcgggc cgtgaaccgg atgaagcgct gatggaacgc
     3841 gtcctgaggc ggcaccttgg cgcggaatga ccgcatgcct cggccctggt tgacttcgcc
     3901 cggcttcacg gtagactgtt gggattcagc gtgcccgcgg cgaaccgggc gcgccattct
     3961 ccggagactt gccgtttgat ggctgagagc gaacgaatcg agccgagccc ggccagcgaa
     4021 accgccgccg ccgacgagca tcgcctggtg cgcgagcgct acgacaaggc cgcgcgcctg
     4081 cgcgccaccg gctgggacct ctacgtcaaa aagcatgagc cgacgcatca cgccgccgat
     4141 ttgcgcgccc gccagcagga actggtggcc tcgggcgagc cggtttccat cgccggccgc
     4201 gtgatgagcg tgcgcgcctt cggcaaggcc ggcttcctgc tcatccgcga cgagaccgat
     4261 cagattcagg cctacgtgaa gaaggatgtg accgacgagc gcggctatga ctattttcgg
     4321 cacggtctgg acgccggcga catcgtcggc gcgagcggca aattgttcta cagcaaaacc
     4381 ggcgaattga ccgtcgaagc ggcccaactg cggctgttga ccaaggcgat ccgccccctg
     4441 cccgaaaaat ggcatggcct gaaggacgtc gagctgcgtt accggcagcg ctacgtcgat
     4501 ctattggcca accccgacgt gcgcgagaca ttccgcgtgc gcagccggac gatcgccgcg
     4561 ctgcgccgct tcctcgacga gcgcggctac atggaagtcg aaacgccaat gatgcagccg
     4621 atttacggcg gcgcggcggc acggcccttc atcacgcatc acaacacgct cgatatggat
     4681 ctctacctgc gcatcgcgcc cgaactctac ctgaagcggc tgctcgtggg cgggctggag
     4741 cgcgtctacg aaatcaatcg caacttccgc aatgagggac tctcgacccg ccataaccct
     4801 gaattcacga tgctggaact ttacaccgcc tggtgggatt acaccgacac gatggacctg
     4861 accgagacgc tgatccgcga gacggcgcgg tcggtgctcg gcgcggcgga actggcatat
     4921 cagggtcatc gactggatct ggggcagccg ttccgccggg tccgcattct ggatgccgtc
     4981 gccgagttgc tgggtctcgc tgcgggcgat gccaatccac tgcgctgggg tctgggcgac
     5041 atggagcggc tgcgggcggc gctcggcggc gcgggcgacg aaatcgccgg gatactgcgc
     5101 gaggaatccg atcccgacga ggcgttgatg cgcgtcttcg aggagcggat cgaaccggaa
     5161 ctgatcgagc cgaccttcgt gatcgactac cccaagagca aatgcccgct ggccaagtcg
     5221 tcccccgcgg atccgcatac ggccgagcga ttcgagctgt tcatcggcgc actcgagacg
     5281 gccaacgcct acagcgagct gaacgacccc cgggaacagc tcgagcagtt tgaatgtcag
     5341 gtgcggcagc gggccggcgg cgacgaggag gcgatgaacg aaatcgatcg cgattacatc
     5401 cgcgcgctgg aatacggcat gccgccggcc agcggtctgg gcatcggcat cgaccggctg
     5461 atcatgctgc tgaccgattc cgcctcgatc cgcgatgtca tcctgttccc gttgatgcgg
     5521 ccggaacgct gagccccgcg gccctccgtg gtgcatcacg ctgtgcttca tcctggtttg
     5581 attggccggt tgaacaatga aatcacttga atggttcatc gcctggcgct acctcgtctc
     5641 gcgcgaacgc aaggccctcg tttcgctgat tacgtttatt tccatgacgg gggtggccgt
     5701 cggcgtcgcc gcgctgatca ccgtcatcgg cgtcatggac ggggcggatg aactgctgtt
     5761 cggcaaggtg gccgaactct acccgcacct gcgcgtggtc aacctcgaag gcccgcgcga
     5821 ggaattcgac ccggaggtca ttcgcaagct gaaagccctg cccggggtga cgcacgttca
     5881 gcccgtgatg cagaagcagg cgttcatcat cgccggccag gggcgcgacc agaaccgcca
     5941 gagcttcgtc caactgctcg gcatggaccg gctcgatcag aacaccattt actcgatcat
     6001 gacctatgag cacggcgatt cggtcacgct gggcgaggac gagatcctgc tcgggctgcc
     6061 gctggcggtg aacatcggcg cgatgatcaa cgaccgcgtc acgctggttt cgaccgcgcc
     6121 ggtgcggacg gccatcggcc cccagatgcg gtttctcaag ttcaccatga aggatgggtt
     6181 ccgcaccgac ttctatgaat tcgacgccaa cacggcgttc atcagcaccg agatgatgcg
     6241 caagctgttc ccgaccgagc cgggctacga ctacctgcat gtcaagctca aggacccgtt
     6301 ccaggccgac cggctcgcgg cgcggttgca cctgcccggc tatcgcatct cgacctggtc
     6361 gcaggaaaac ggggcgtttt tcagcgcgct gaagctggag aaactcgcgc tgttcgtgat
     6421 tctgctgctg atcattttcg ttgccgcgct caacatcatc ggcaccctga tcctgatggt
     6481 gaacgacaag acgcgcgaaa ttggcatcct gaaagcgatc ggtgcatcgc gccgcacgat
     6541 ctggaaaatc ttcctgctcg acggggtgat gatcggcatc ctggggaccg gcgcgggcgt
     6601 ggcggtcggc ctgctcctct gtttttcgat cccctatatc ccgcttgacc tgcccgcctc
     6661 ggtctacaat ttctcgcgtc tgcccgtgga agtacagccc gtcacggtgt tgctgatcgt
     6721 ctgcagctcg atggtgatct gcacgctggc ggcgctcttc ccggcgattc aggccgcgcg
     6781 gctgcatccg gtggaggccc tgcgctatga atagcctgct gaccgcgcgc gatatttata
     6841 aaagctacca cgacggcgag cgggaactgc gcgtcctgcg cggcgccagc ctgagcgtcg
     6901 cgcgcggcga ggccgtcgcc gtcgtcggca tgtcgggcgc gggcaagtcc acgctgctgc
     6961 atttgctcgg cggcctcgat gtccccgacc gcggcgagat cgccgtcgat ggcgagacga
     7021 tcagcggtcg ctcgttgacg ttcctgcatg agttccgcaa ccgcaagatc ggctacgtct
     7081 ttcagttcca tcatctgttg ccggagttca cggcgctgga gaatgtgatg atgccggcct
     7141 tgatcgccgg cggtcagcgc aagacggtcg ccggcgaggc gcgccagatg ctcgagcgcc
     7201 tcggcctggc ggatcgcgag cgccaccggc cgagcaagct gtcggggggc gagcagcaac
     7261 gcatcgccct ggcccgcgca ttgatgaacc agcccgcgct gttgctcgcc gacgaaccga
     7321 ccggcaatct cgacctgcac accggcgacg tcgtgatcaa tctgatgtgg gaaatgacgg
     7381 tcaagcaggg gcgcggactg gtcatcgtga cgcacgagcc cctgatcgcg gcgcgcgccg
     7441 accgcgtgct gcgcctgcac gaggggacgc tgctggaggt ggccaggaac gagattgagg
     7501 cgcagatggc tcagggcccc ggcgcaaagc cttagccgca tacctgaata cttcgctcgt
     7561 tagtcctcgc tcgaaaattt gtctccgatt cccggaatcg cctggattgg ttctgccgtc
     7621 cctacgggat tcggtcgcgc tggggatccc ggttcccgcc actgacgtgg cgggctgtcg
     7681 tctgccgtcc ctgacgggac tgccctgctg ttagatccgg cgagccctgg atcattcgaa
     7741 tcagccgttc atccccgcca ggttcatccc tcgttgtgaa ccgcaggttc atgagcaact
     7801 cggaataatc tacctggacc tgtccgcctt cacgtgaccc gcgcgcccaa ctcgtgatac
     7861 agcgtcagaa tctgctcgcc gttgtgcagc caggaatgct ccagttcggc gcgacggcgc
     7921 gcggcatcgc ccatggtgcc cgccagcgcc ggatcggcca tcaggcgcag cacagctttc
     7981 gcgatctgct tcacgttgcc cgccggatag atcagccctt cgcgcccgtc ctccagcgat
     8041 tcgaggatgg ccggataggc cggcgccagc accggcacgc cgcaggcctg atactcaaac
     8101 aacttggcgg gggaagacca gtaggttgtg tcgggcacga tcgcggcatc catcgcgcgg
     8161 atgtgggccg gcacgtcgtc gtggggcacc gacccgacaa accgcaggac gtcctccagc
     8221 cccgcctcgc gcgccttgcg gcggtgcatc tccaatttga gcccatcgcc cccgacgatc
     8281 aggaaggcat agccggtcgt ggcccgcttg cgcagccggc gcgcgacatc gtacagcagg
     8341 cggatgccgt gccagccggc cagcgtgccg acgtagccga tgacgaaccg gtcgtgcaga
     8401 ccgtggcgcc ggcgcaattc ctcgcgcccc ggcccgtccg ggtgaaattt gtccagattg
     8461 acggccatcg gcatcttggc gatggccgcc atcgattgcc ccagttcatg cacctcgcgg
     8521 cgcaggggtt ccgaaacaac gatgacccgc ggcgcgcggc ggatgatgtc gccttcgacg
     8581 cgacgcgcca gccacgccag ccggatccgc tggtgctgct ccttggtcag gaaggcattc
     8641 acttcaagca gccgcggcag cccgaattca gccgtggcgc gggtccccgt gcgcgcatag
     8701 agcgagtatc gctcgtagat cacgtcggga cgccactggg cgaagtcggc ccggatgcgg
     8761 cgccacaggc gctggtcggt caaaatgtgg cgcaggtcga accccagctt tttggactga
     8821 accgggcgca cgtggaccac gtcgagcggc ggcccgtcgg tttcatcccc ccccacgttg
     8881 gtgcaccaga tccgcacgct gtggccgagc gacatcagaa tccgggccgt ttcgcgaacg
     8941 tgggtcgaac accccttgcg tccgtaaagc gggatgcccg ggtcggcgca gagataggca
     9001 atacgcatgc gggatggcgc ggcgagcggc cgcttcctca ggagtggccg accggtccaa
     9061 gcccggcggg gcgcgccgcc tcgggcgacc ggctggcgat gtcgatcagc cgcagcattt
     9121 cggccgtggc cgaccgcagc ccgacgaaca ccgcccggct gacgatcgca tggccgatat
     9181 tgagttcctg caggtgcggg atggcggcca ccgcctgcac gttggagtag ttgagcccat
     9241 ggccggcgtt gagcaccagc ccggcggcgt gaacctgccg ggcggcgtga tacagcttgc
     9301 gcagctccgc caggcgcgcc tcgggcttgc gggcgagcgc atactgtccg gtgtgcagtt
     9361 cgacctcgtc cgccccgcaa gcaagcgagg catcgatctg gtccccttcc ggatcaatga
     9421 acatgctgac gcggatcccg gcgtcccgca gccgttcggt caccttgcgc agcgactcca
     9481 ggttgccccg cacatccagg cccccctcgg tggtcacctc ctcgcgccgc tcgggcacca
     9541 gcgtggcgat gtcgggccga atccgcagcg ccttttcgac gacgctctcg gcggcggcca
     9601 tttccaggtt gagatgcccc tggacgacct cccggatcag gcggacgtcg cgctcctgga
     9661 tgtggcgctg atcctcgcgc aggtgaaccg tcacgccgct ggagccgccc agttcaacct
     9721 cgccgaccgc gaaaacgggc tcgggttcga tcccccgacg cgcctgccgc agcgtcgcca
     9781 cgtggtcaat gttgacacat agcctggcca tgagagtgtc cgcttgcgaa ggtgatcatt
     9841 gcccgtcccg ggcgccgtcg gggaggcccg cgccggggcg gaggggcgcc gactcagtcg
     9901 cgaccgggag ttccggctca gcgcccgcct catcttcccc ggacggcgtc acaaactcaa
     9961 cttcgattga cgtcggccgg gtctcgatga tcttgacccg ctggatcaca tcgggcggaa
    10021 cgctctgacg gaagcgcgcc tccaggccga ctttctgcac gttgcccacg cgctcttcca
    10081 gtccgcgctg gggactgagc aggatgtcct cctgggtcag acggttcagc gccgagggtg
    10141 gtccctccac gacgacgttg gccgtcggcg gtcgcacccg ggcggcgatg ttctcggcgg
    10201 cgatcggcac gttgatcgcc acgtcgttca aggtccgctg ctcgctgact tcctccaccg
    10261 cgatgtcgat cgccacccgt tcctcctcgc gttgcaggat cttgacccct tcggggatca
    10321 ccagattggc gaagatatgg ttcgactgcg tgatcttgcg caggtcgatc ggctcggtca
    10381 ggatctggtt ctgctgaaga gcgagagcat tgaacccgtc ggtcgtgccg gtgacgagca
    10441 gttcgggggg cgtcgcgcgg atctcgccca ccagccgcag cgtcggcggc aactcgccca
    10501 ccgtgtacgg aacgacgcgc acccggcgcg tcaccaactc cgcatcaagc gtgacccggt
    10561 ccggttcgat atcgatcacc tgcacccgtt gcgaaagcga cggccgccgc accatgtcct
    10621 ccgaaaggct gtactccaca tccaactgtt ccggggtctt ccaggtggcg gggtcctcgc
    10681 ggaagacttc ccgcacgttg atgcgcagcg aaaaattact cggcacgatg ctgttgcgca
    10741 gatccaccgg gtactggacc gcgatccgga cgaactgcgg cgagatgcgc acattcatga
    10801 tctggccctc gttctcgagc tggaccggga tctccagccg gtcgatctcc agctccccct
    10861 gtttggcgat cagccagatc acgaacgcga tgaacagcgc catgccgatc aggacaaatt
    10921 cactgcgggc gcgcgaagct tccatcaacg cgcctcctcg ccctgcgcct gctggtgggc
    10981 aatctccagt tcttcctcga gtcggctgcg cagctcggcg ggcttcagat tgcgctggat
    11041 ctgcccattt tcagccagcg agatcgttcc cgtttcctcc gagacgacaa taacgacggc
    11101 atcggtctcc tccgacaggc cgagcgcggc gcggtggcgc gtgccgagct cgctgctcag
    11161 ttcgggattg tccgacagcg gcaggatgca gccggcggcc agaatgcgct cgccgcggat
    11221 caacatcgcc ccgtcgtgca gcggggagta gggcgtgaag accgtgcgga tcatctcggc
    11281 cgagacggtc gcatcgagca ccgtgccgga gcccatgtag ctgcgcagcg ggttgcggcg
    11341 ctccagcacg atcagcgcgc cgatgttgcg ccccgccagc agcgtcacgg cgttgatgat
    11401 ctcgtcgagt acgtcgcgct cacggctcgg aaacaggccg cgcagcaggc gcaactggcc
    11461 caggtcggtc aacgccttac ggaactcatt ctggaacacg atcaggaaga tcaacacgac
    11521 gacgatccag aagttctgaa aaatcaggat catcgcctgc aggtcccacg tcagcgcgat
    11581 caggaacagc agcagcgagg ccagggtgaa ggtgacgaaa cccatcagcg cgacgaacga
    11641 ccgcgtctcg cgcagaatcg cgaaaatgcc gtagaagaac agcgcgacca ggaggatgtc
    11701 ggccaggtcg agcagccgaa agttcatgat cgaagtggtg agctcttgca tgccaggggg
    11761 ctccgggtcg catggctccc gagtcgcgcg cgtcgcggcc gtgaggcaag gcgattattt
    11821 caagccccat gaggcgttgc aagcctgctc gcccgccggg cgaccgtcga tgatcctccg
    11881 ccgccggcct ttgctgcacc tttagggacg ggagagacgg ctgagcagcg ggggacgccg
    11941 cggaagccga aggcggctcg ccgcctcaag cgttgcgctg gacccgaccc gccacgaccc
    12001 gatctacccc tcaccgtgcg gtcccaaccc tgaccgcgcc gcccaacccg caataagccc
    12061 ccctcgcccg ggggtatata gagccttgac agccttttcc aaccccctcc caatgggcct
    12121 atcccatttc acgattggct ccccgcgccc gcccacgggg gggtcaggtt ctacctgccc
    12181 ttgcccttga caccccccat ttcccgttga cacgcctcgc cccctccggt aaaaaggtcc
    12241 gtttgtgtgg cgtggtgtgt cctgttctca gggatgcatg gggttccccg cccgcgcggc
    12301 ggcacggttc tcagtgaccg gcaccaccca cggagcccca ccggatcaca tcgctcggaa
    12361 agacctccaa tcatgggtta caccaagtcc gccaaagcgg aagctttgcc ggaaaagtgg
    12421 tacgtggtcg acgcgcgcga tcaggtgctg ggccgcctgg cgagcgacgt cgcccatgtc
    12481 ctgcgcggca aacacctgac caattacacc ccgcacgcca acatgcggac ccacgtcatc
    12541 gtcctcaacg ccgacaagat ccgcctcacc ggcgataagt gggaaaccaa gctctatcat
    12601 catcacaccg gctgggtcgg tcacctgcgc gagaccaacg cgcgcaagct caacgaacgc
    12661 aagcccggtg aactggtgcg caaggcggtc tggggcatgc tccccaagaa ccgcctgggt
    12721 cacgccacga tgacccgcct gcggatcttc agcggcgacc agcacccgca ccaggcgcag
    12781 aagcccgaac ccctgcccac ccggcaggcc gccgcccagt ccgccgcccg ctgagcggcg
    12841 ccgccaaacc tttcgcttga accactagcc gaggagatga catggagcag tatctgggaa
    12901 ccggacgacg caaaacctcc gtggcgcggg tttacctgcg gcccggccgc ggccgactca
    12961 tcatcaatgg cctggcgccg gaggtctact tcagcaagcg cccgatggtc gaggatgtgg
    13021 ttcgcgcgcc gctgcgcgaa acccacaccg tcagcaaata cgacgtcgtc gtcaacgtct
    13081 tcggcggcgg cgtcaagggc caggccgaag cgatccgcca cggcatcgcc cgcgccctgc
    13141 agaacgccaa tcccgcctat cgccccaccc tcaaggcgct gggcttcctc acccgcgacg
    13201 cccgcgagaa ggaacgcaag aaatacggcc tgcacaaggc ccgcaaggca acccagttct
    13261 ccaaacgctg atccttccgg attgggattg acgctgatcc ccccgcgttg ggattggatc
    13321 atcacaaggc cccgccgcca cccccggcgg ggctttgcat ttgaaatctc gtccctcatc
    13381 cctcgtcctc gtcctcggcc tcgcctcgcc ttcccccccc tcccgcatcc cgccaggaac
    13441 gccagatatt gcgcgcggcc tgtaacccgt ggtcgtcatc accccccccc c
//
LOCUS       DAOPCO010000083        13288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4659_length_13288_cov_5.554674, whole genome shotgun sequence.
ACCESSION   DAOPCO010000083 DAOPCO010000000
VERSION     DAOPCO010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13288
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4659_length_13288_cov_5.554674"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            720..4361
                     /locus_tag="PLS90_07670"
     CDS             720..4361
                     /locus_tag="PLS90_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02321.1"
                     /translation="MDPSVCTARSGASWLALLLIIAFACRPPSAGAQTDFNEDSLFDV
                     GDVTTLELRIRQAPAAPEDQLELDVNRSGTLDQLDPRHLANVLIGAANALPGIFIGGG
                     LGNLFITNQAALTFAIEIENANGPFEVLLRSGEVLFSGITSKTGQITLTDLDEGANLI
                     ELIYRDDTGTSFTRTLEVRRDVTPPQLSIESPLPDVVVQSLTVAVTGQAIDEQGLAVV
                     TVNGVPMTLESDGSFHGEVRFQTSGPVALRARAVDTAGNVTRAERLIEMYVSPPDSMT
                     ESEVRIDLPAGTINRNGEEAFIDTELNSDIKALLGPETEGLVNTMPAEGLTRGIIIMP
                     NAMMVSVETGVTPEEGLLGSPPAPMFANQPVISLQNTSGADNSMPLWIFQVIPDTDGD
                     GFPELSLAARAKVAEDGPAAGRVVPIEPEGDDPLPGTFNPEIPVFTETTLKQVESLGS
                     IQRLKQRMKAREAKGETPTARVTFYCCAASAVIPVKGKVRCGSLTLDDVFSRLTDIEA
                     ELFLLKGDIDRRTKRFNEAGLAGEDAAKDILGVAKIPVLGSNQVIEVPTVVNGKAFSC
                     LKNFVTGGAAAIPEAIGKVEDTIKALDDIRLLIGQEDPPPPVADIVARLAERGLDYQH
                     YHILQSVLHSDLTPGAFDDGIEKLTRIKQAKVILGNIGAVKDCIGLLTGVTKVISSWI
                     TIESEDELSDAEFRLYDNLLMRYKRMQDCKRHLIAVQDAALEGSALPGYLQYKTDRKA
                     VLDDLNGLQAFIGNSMDWEHALLDLTDDLIALLEKNEQQVTLTELEALVDHFEQTLLM
                     EKNLLERHEEAKNGLDAVMQVKEQLPVVVAQATAKNEAVISSLDAAVADRGGYAGASF
                     SFAGAGAPFTVVSGAGGDFSHFLFTDLDVDPIGGENRMARLTGRDYTGEAVAEDGLYT
                     GESSGQVETLEFPFTQFSTVDFDASIDIGDILLGESVEDAGGQAPTVQILFPPADFQI
                     PAGFPVRVQTEATDDVAVLGVEVSVDGESQIGLPGGVSEGILRMPGELGPVVIKAAAI
                     DPGGRITTTERTVQIVDAEGAFVIDPPAIRIEGGETQDFEALFAGLPASGVTWKVNGF
                     PGGQPAVTGTIDAAGFYDSGPADFGGVAAALVQVSAELDAFPGFEASARVVVVDSLNV
                     IAPPLAFSFPAPGAPGGLLSSRPLAFSFPAPGAPGGHLVSTPLAFSFPAPGAPGGWLP
                     TPPVAFERQ"
     gene            4438..5868
                     /locus_tag="PLS90_07675"
     CDS             4438..5868
                     /locus_tag="PLS90_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02322.1"
                     /translation="MRTLWTGLVMILAWGAATLAASAQGLAFDSGSDGSDGELVVPDS
                     TVQVLNMNDGLHDDGIYNFTSVTINAGATLEITPNDNNTPIVILVQNDFIMDCAAKIL
                     VNGRDGGIPNPGVGGPGGFDGGVGGVDNVVPQLPGPGLGPGGGIPNLEGDDMGLDGGR
                     NNFMRPSLVPLIGGSGGGGAFATLGSGNSGGGGAGGGGAILIAASGRFELSTCSGSLI
                     DASGGNSLNSGGSVHSGGGGAGGAIRIIASQVDGGWLIDAEGGVGGGTFGEKGGDGED
                     GIIRIETFRLTRNFNIVRGRMFNSLPGIVDGDAADLSTLRISSIGGVAVPDDAGANVA
                     LPDVIIPAGVTNPVRIDVEATNVTPGQTAMVRLNYTGNGGPIIEAETSALAGTAALST
                     AFVDVNLAAGSGTIAAILRTDAFTPQIPPAAPQEQAALNIFENMRVTLDGEPVEFIER
                     TREVGQAGERVEYVTASGRRAAVPVP"
     gene            complement(5890..6315)
                     /locus_tag="PLS90_07680"
     CDS             complement(5890..6315)
                     /locus_tag="PLS90_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02323.1"
                     /translation="MKRIPLIVAWLSLGAVFTGCAFNSVYWTPPPPTVVHESDLASPP
                     GPGQAKYLYYYQREVYFNLDDNRYTYPWQGSWHTHARLPWIFRGDLGSYVVLDLPREK
                     PERFHAQVLEQFPPSFPILVVSSDESMSPPAIPAVPGYE"
     gene            complement(6892..7311)
                     /locus_tag="PLS90_07685"
     CDS             complement(6892..7311)
                     /locus_tag="PLS90_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02324.1"
                     /translation="MRHRTALATLFAGLILVGTGLTGCTTYNSEYYRSGRSRPYYDRH
                     DHVRERVIVREVPRRDRHYHQTIIRHDRHDRGRGPVWRPDRNDHRRDHVRHNDRRDWD
                     RRRDWDRRGNDRDRRDHRDNNRRGGGNDRRGGNNRRH"
     gene            7482..8681
                     /locus_tag="PLS90_07690"
     CDS             7482..8681
                     /locus_tag="PLS90_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPK02325.1"
                     /translation="MNKSIDAIAAGHICLDITPIIPETGRARIEEILTPGSLTMVEAG
                     TVALGGQVANTGLALKRFGHSVAALGGIGDDELGLVVQRLLERHAAAGAIRPAPGAVT
                     SFSVVLAPPGIDRMFLHCPGANDTFGAENIDFDLVRRARLLHFGYPTLMRRMYADRGA
                     ELARIMRQTKDCDVTTSLDMSMPDPHSPAGLADWPRILEQTLPWVDLFLPSVEEIAVA
                     LEREDYLARRRRDPAADLVEHYTVADFRRLADRALAMGCGVCVIKAGSRGFYLRSGPA
                     ERLARFGGAARCEAGPWADRELWCPAYRIDRIASATGSGDCAIAGLLAGVLRGLAPER
                     AMRLACAAGWHNLRALDPLSGLPDWAGLEAFVDSGPPVLDPRLSSDEFNWKPGAEVYV
                     GTRDARR"
     gene            complement(8887..10047)
                     /locus_tag="PLS90_07695"
     CDS             complement(8887..10047)
                     /locus_tag="PLS90_07695"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HPK02326.1"
                     /translation="MRFTPGSSARRRVRGPLFALTLALLLPCATPRAEIVKMVSLLTG
                     AQDGVVSDALGVGHYTIDTDANTLDYEIRFGGLSSSETAAHFHGPADPGVDAGPIHTL
                     ALGALKTGTWNYDEGQEADILGGRVYVNVHSSNHAPGEIRGQVVDMVALLDAAQEDNP
                     SNTSTASGVGLFMIDRDANTLDYYILFDGLVAAESAAHIHGLSLHGTASSPLHTLAAG
                     SPKDGSWNYNENQEAAILSGLTYVNVHSTTYPGGEIRGQITNNLAFLDAAQEGGSVSS
                     SAYGVASFSHDPANLQLGYYITYADLTSAESVAHIHGYAAPGSPAGPLHTLPTSNPKL
                     GVWDYLGALAGPMDQLYVNIHTSNNPGGEIRGQLQPVENVVVPVELSTFGMQ"
     gene            10503..11120
                     /gene="hisH"
                     /locus_tag="PLS90_07700"
     CDS             10503..11120
                     /gene="hisH"
                     /locus_tag="PLS90_07700"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPK02327.1"
                     /translation="MPPRVTIIDYGMGNLHSVSKALEHLECAAEITDDPARVREARRL
                     ILPGVGAFGDAMRELERRRLVEPLLDRVAAGVPLLGVCLGMQLLLETSEESPGVAGLG
                     LIPGTVRRFRTDLKVPQMGWNTVRQVRQAPLFAGIPDESWFYFVHSYYVAPANGAGGA
                     VAGVTDYAGEFPSVLWRDHVQATQFHPEKSQSLGLQMLRNFTRME"
     gene            11183..12115
                     /gene="thrB"
                     /locus_tag="PLS90_07705"
     CDS             11183..12115
                     /gene="thrB"
                     /locus_tag="PLS90_07705"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPK02328.1"
                     /translation="MAATGDLIIRVPASTSNLGPGYDCLGLALNLYNDFTVQVNRRPG
                     ARSTVTGRGRCAGIPAEDNPFVRAYAAVGEMAGADLPAVSVTVDGDVPLGVGLGSSAG
                     GAVAGALAANALLGLPFTYEELIEPLVRVEGHPDNVTPSLLGGLTVSTATDAGPLTHV
                     YQVAEAWRLAFLIPDYVVETTAARGALPSRVPLADAAFNLSRVPFVIHALVAGDGEML
                     GRVMEDRLHEPVRGKMIKRDKRIRRAARAAGACATFISGSGPAIGAFCLGQETAERVA
                     EAMRAETEGAKFTAEALVLRPKRGGARIAEGDQP"
     gene            complement(12112..>13288)
                     /locus_tag="PLS90_07710"
     CDS             complement(12112..>13288)
                     /locus_tag="PLS90_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02329.1"
                     /translation="IERKLLRHPGVEIDILRDYIMVYEWIRGIDAAEVFQQLRKPTEE
                     LHALTHRVRGELAAKGFVVADMKPAHIIVRKRNDGSLASRRGQVVYALVDFELLGRTP
                     EYEEERRRIRRAEYHLFQERRFSGPDVPPLPAHLWPVTIFGVDYVYGVVESTGGRLWV
                     VGHDARLYDYFQPERWRKTPCVQLSSRHQVFYTRTKDHINLVWKTSRVGEKPDLEIPE
                     AAALAEQGYNSPFEEFAIALELERLGVPVTQPRAIYKTGSTSANPDYLADRRRFDSHA
                     ALRTPDGEPILRPDHDYIKVWGFWNGPEEVPADLTDIHRHALNADQAVREGRLKPAQV
                     HEMVEWMKERLAAAGYGTPWLASHHLLLSRDARGRFHRDIHGRIECRLCNFEFVRKI"
BASE COUNT         2157 a         4356 c         4591 g         2184 t
ORIGIN      
        1 gatgatcgcc agcgggaaga gcgcatcccg attgcgctgg agcgcatgga ggaactgatt
       61 gggggaaccg gctgggggag cgattgtcgc ggccgaggcc atctgccgtc gccttcgcag
      121 gggaatagac agtcagatcc ctgacaacag ggctgccggc ggcggatgat gcaacatgtc
      181 ggccagaagc ggaatgagcg cgatgagcca ggcggagagg agtcagggcc gctccgcccg
      241 cggaggtcgt atatcatata aatcatttga tatcagtcat gaatgttcaa agaagggcgc
      301 ccgccgaggc aggccggctg aaagcggcga tggaggtcca tacgagattt tggcgtgaga
      361 gctgacaata atttggcgaa ttccggtcca agcagcaggc tgttggtcaa ataattgacg
      421 aatccagccc cggtgcggtc gcggcgtttc tcccacccgt atctgctcct tctcaggtat
      481 ctcctatgtc cctgggatcc gttccaccct gccgcgccta acccgcgcaa tctggaaaat
      541 tcatgattat gccatcaaga aagatcttgc ctcgccacgc cggttgcctt acccttgcct
      601 ccgcagtctg acagtgtttg ccggtcacgc gctgatccag cgggggtgac ccaaccggga
      661 cgcctggcgg ccttgagtgc gtccttgtgc atccgtccag gtgtttcgcg agggtttccg
      721 tggatccatc ggtttgcacc gctcggtcgg gagcgtcgtg gctggcgctg ctgttgatca
      781 tcgccttcgc ctgccggcct ccctcggccg gcgcccaaac cgacttcaac gaagacagcc
      841 tgttcgacgt cggcgacgtc accacgctgg agctgcgcat ccgccaggcg cccgccgccc
      901 ccgaggacca gcttgagctg gacgtcaacc gcagcgggac gctcgatcag ctcgacccgc
      961 gccacctggc caacgtcctc atcggcgcgg ccaatgccct gcccggcatc tttatcggcg
     1021 gcggcctggg caacctgttc atcacgaacc aggcggcgct gacgttcgct atcgaaatcg
     1081 agaatgccaa cggcccgttc gaggttctgc tccgctcggg cgaggtcctt ttctcgggca
     1141 tcaccagcaa gaccggccag atcacgctga ccgatctgga cgaaggcgcc aacctgatcg
     1201 agctgatcta ccgcgacgat acggggacgt cgttcacgcg gacgctcgaa gtccggcgcg
     1261 acgtgacccc gccgcagctg agcattgaaa gcccgctgcc cgacgtcgtc gtccagtcgc
     1321 tcacggtcgc cgtcaccggc caggcgatcg acgagcaggg cctggccgtc gtgacggtca
     1381 acggcgtgcc gatgaccctg gagtccgacg gcagcttcca cggcgaggta cggtttcaga
     1441 cgagcgggcc ggtggcgttg cgcgcccgcg cggtggatac ggcgggcaac gtgacgcggg
     1501 ccgagcggct gatcgaaatg tatgtctcgc cccccgactc gatgaccgaa agcgaggtgc
     1561 ggatcgacct gccggccggc acaatcaacc gcaacggcga ggaagccttc atcgacaccg
     1621 agctcaacag cgacatcaag gcgttgctcg gcccggagac cgaggggctg gtcaacacga
     1681 tgccggccga gggactgacc cgcgggatca tcatcatgcc caacgcgatg atggtgtcgg
     1741 tggagacggg cgtgacgccg gaggagggcc tgctgggatc gccccccgcg ccgatgttcg
     1801 ccaaccagcc ggtgatctcg ctgcagaaca ccagcggcgc cgacaattcg atgccgctgt
     1861 ggatcttcca ggtgattccc gacaccgacg gagacggctt ccccgagctg tcgctggcgg
     1921 cgcgggccaa ggtggccgag gacgggccgg ccgccgggcg cgtcgtgccg atcgaacccg
     1981 agggcgacga tccgctgccc ggcacgttca atcccgagat ccccgtgttc accgaaacga
     2041 cgctcaagca ggtcgaatcg ctcggctcga tccagcgctt gaagcagcgg atgaaggcgc
     2101 gcgaggcgaa gggcgaaacg cccaccgcgc gcgttacgtt ctattgctgc gccgcgtcgg
     2161 ccgtgatccc cgtcaagggg aaggtccgct gcggctcgct gacgctcgac gacgtgttct
     2221 cgcgcctgac cgacattgag gcggaactct tcctgttgaa gggcgacatc gaccggcgca
     2281 ccaagcgctt caacgaggcg gggctggccg gcgaggatgc cgccaaggac atcctcggcg
     2341 tggccaagat cccggtgctc ggctcgaacc aggtgatcga ggtcccgacg gtcgtcaacg
     2401 gcaaggcgtt cagttgcctg aagaacttcg tcaccggcgg cgccgccgcc atccccgagg
     2461 cgatcggcaa ggtcgaggat acgatcaagg cgctcgacga catccgcctc ctgatcggcc
     2521 aggaggatcc gcctccgccc gtcgccgaca tcgtcgcccg cctggccgag cgcggactgg
     2581 actaccagca ctaccacatc ctgcagtcgg tgctgcactc cgacctgacc cccggggcgt
     2641 tcgatgacgg gatcgaaaag ctgacgcgga tcaagcaggc caaggtgatt ctgggcaata
     2701 tcggcgcggt caaggactgc atcggcctgc tgaccggcgt caccaaggtc atctcgtcgt
     2761 ggatcacgat cgagtcggag gacgagctct ccgacgccga attccgcctc tacgacaacc
     2821 tgctgatgcg ctacaagcgg atgcaggact gcaagcgcca cctgatcgcc gtgcaggacg
     2881 cggcgctcga ggggagcgcg ctgcccggtt acctccaata caagaccgac cgcaaggccg
     2941 tactggacga tctcaacggg ctgcaggcat tcatcggcaa cagcatggat tgggagcatg
     3001 cactgcttga cctgaccgac gacctgatcg cgctgctgga aaagaacgag cagcaggtga
     3061 cgctgacgga actggaggcg ctggtcgatc acttcgaaca gacgctgctg atggagaaga
     3121 acctgctgga gcggcacgag gaggccaaga acggcctgga cgccgtgatg caggtcaagg
     3181 agcaactgcc ggtggtggtg gcgcaggcga cggcgaagaa cgaggcggtc atcagctcgc
     3241 tcgacgcggc ggtggccgac cgcggcggct acgccggcgc gagtttttcg ttcgcgggcg
     3301 ccggcgctcc gttcaccgtc gtcagcggcg cggggggcga tttctcgcat ttcctgttca
     3361 ccgacctcga tgtggacccg atcggcggcg aaaaccggat ggcgcggctg accgggcgcg
     3421 actacaccgg cgaggcggtc gccgaggacg gtctgtacac gggcgaatct tccggccagg
     3481 tggagacgct ggagttcccc ttcacgcagt tcagcaccgt cgatttcgat gcctcaatcg
     3541 atatcggcga catcctgctc ggcgaatcgg tcgaggatgc cggcggccag gcgccgacgg
     3601 tgcagatcct cttcccgccc gctgatttcc agatccccgc cgggttcccc gttcgcgttc
     3661 agaccgaggc gaccgacgat gtcgccgtcc tgggcgtgga ggtgtcggtc gacggcgaat
     3721 cgcagatcgg cctgcccggc ggcgtgagcg aggggattct gcgcatgccc ggggaactgg
     3781 ggccggtggt catcaaggcc gcggcgatcg accccggcgg acggatcacg accaccgaac
     3841 ggacggtcca gatcgtcgat gccgaagggg cgttcgtcat cgatccgccc gcgatccgga
     3901 tcgagggggg cgagacgcag gatttcgaag cgctcttcgc gggcctgccc gccagcggcg
     3961 tcacctggaa ggtcaacggg ttccccggcg gccagccggc ggtgaccggc acaatcgacg
     4021 cggcgggatt ttatgacagc ggtccggccg atttcggcgg cgtcgcggcc gccctggttc
     4081 aggtctcggc cgaactggac gcgttccccg gcttcgaagc gagcgcgcgc gtggtggtgg
     4141 tcgattcgct taatgtgatt gccccgccgc tggcgttcag tttccccgcg cccggcgcgc
     4201 cggggggcct gctcagttcg cggccgctgg cgttcagttt cccggcgccg ggggcgcccg
     4261 gcgggcatct cgtctcgacg ccgctggcgt tcagcttccc cgcgcccggc gcaccggggg
     4321 gctggctgcc gacgccgccc gtggcgttcg agcggcaata gcgggggacg ggcacgagag
     4381 acgagggacg aattcaggac tgagggaaga atcgcacggg aaaaggagaa tcgaacgatg
     4441 agaacccttt ggacgggact ggtcatgatc ctggcgtggg gcgctgccac gcttgccgcc
     4501 tccgcgcagg ggctcgcctt cgacagcggc tcggacggct ccgacggcga actggtcgtc
     4561 cccgacagca cggttcaagt gctcaacatg aacgacgggc tgcacgatga cgggatctac
     4621 aatttcacga gtgtgacaat caacgccggt gcaacgctcg aaatcacgcc caacgacaac
     4681 aacaccccga tcgtgatcct cgtccagaac gatttcatca tggactgcgc ggcgaagatc
     4741 ctcgtcaacg gccgcgatgg cggcatcccg aatcccggcg tgggtggtcc gggcgggttc
     4801 gacggcggcg tcggcggcgt ggataatgtc gtgccgcagt tgcccggacc gggcctggga
     4861 ccaggcgggg gcatccccaa tctggagggg gacgacatgg ggctggacgg cgggcggaat
     4921 aatttcatgc gcccctcgct cgtcccgctg atcggcggtt cggggggcgg cggcgcgttc
     4981 gccacactcg gctcgggcaa cagcggcggc ggcggcgcgg gcggcggcgg cgcgatcctg
     5041 atcgccgcct ccggccgatt cgaactgagc acctgttcag ggagcctgat cgacgcctcg
     5101 ggcggcaact cgctcaattc tggcggctcc gtccattcgg ggggcggcgg cgcgggcggc
     5161 gcgatccgca tcatcgccag ccaggtcgac ggcggctggc tcatcgatgc cgaagggggc
     5221 gtgggcggcg ggacgttcgg cgaaaagggg ggcgacggcg aggacggcat catccggatc
     5281 gagaccttcc gcctgactcg caatttcaac atcgtgcgcg gccgcatgtt caacagcctg
     5341 cccggcatcg tcgacgggga tgcggccgac ctttccacgc tgcgcatcag cagcatcggc
     5401 ggcgtggccg tccccgacga cgccggcgcc aacgtggccc tgcccgacgt gatcatcccg
     5461 gccggcgtga ccaatccggt ccggatcgac gtcgaggcaa ccaatgtgac gccgggtcag
     5521 acggcgatgg tgcggttgaa ctacacgggc aacggcggcc cgatcatcga agccgaaacg
     5581 tcggcgctgg ccggcaccgc ggcgctctcg acggcgtttg tcgacgtgaa cctggccgcc
     5641 ggcagcggca cgatcgccgc catcctgcgc accgacgcct tcacgccgca gattcccccc
     5701 gccgcgcctc aggaacaggc cgcgctgaac attttcgaga acatgcgcgt gacgctcgac
     5761 ggtgagccgg tcgaattcat cgagcggacg cgcgaggtgg gtcaggcggg cgaacgtgtg
     5821 gagtatgtga cggcgtcggg ccggcgcgcc gccgttcctg ttccctgaaa cgaaggagcg
     5881 accgcgcgat tattcgtagc ccggcacggc ggggatcgcc ggcggcgaca tcgactcatc
     5941 gctgctgacg acgaggatcg ggaaggacgg cgggaattgc tccagaacct gcgcgtgaaa
     6001 gcgctcgggc ttctcacgcg gcaggtccag caccacgtag ctgcccaggt cgccgcggaa
     6061 aatccagggc aggcgcgcgt gcgtgtgcca gctcccctgc cagggatagg tgtagcggtt
     6121 gtcgtcgagg ttgaagtaga cctcgcgctg gtaatagtag aggtacttgg cctggccggg
     6181 gccggggggc gacgccaggt cggattcgtg cacgacggtg ggaggcggcg gcgtccagta
     6241 aacgctgttg aaggcgcagc cggtgaaaac cgcccccaga ctcagccatg cgacaatcag
     6301 aggaattcgt ttcataccgc gtccgccgtc tctcaactga attccgtacc ctgtccccgc
     6361 cctcgaccgg tctccttgag gtcgatcaat ccagttagct tcaggtctct tcgcgccaat
     6421 gtcaatcacg acatgaatcg ccgcggcggc gcggcgcaag acggctcgtg ccatccggca
     6481 ttgggggcgc ttgatctggg cggggagcga ggtcgaggca actcttgtgc ggtggcagag
     6541 cgttgcagcg caacgcgtcg acaccgcttt cgattccgat gaccaggaca gatgctgacc
     6601 gatccaaagc ggcgtcgcca agcgctgcgc gctttctgcc gccgcactcc acgagtgcat
     6661 cctgcttcca cggtcttaac gcgacgtctg aacgactgga ccactcccga accgtgtcaa
     6721 gttgcgcccc ccgcggcggc gcggggcgcg tggtgttcga tcgggccaat cgaatggggc
     6781 atggccatcc tggccatggc tgggaggggc ggcgaatggc gctcgccccg gctcatgagc
     6841 aggatggcca tgtcacgtca aggcgcgggc ggaatcagcc tccgcggcgc ctcagtgtct
     6901 tcgattgttg ccgccgcgcc ggtcattgcc gccgccccgc cggttgttat cgcgatgatc
     6961 gcgccggtcc cgatcgttcc cgcgccggtc ccagtcgcgc cggcggtccc agtcgcgccg
     7021 gtcgttgtgg cgcacgtggt cgcggcgatg atcattccgg tccgggcgcc acacagggcc
     7081 ccggccacga tcatggcggt cgtgccggat aatcgtctgg tggtaatgcc ggtcgcggcg
     7141 agggacctcg cgcacgatca cccgctcgcg cacatggtcg tggcggtcgt agtacggccg
     7201 cgaccggccg ctgcggtagt actccgagtt gtaggtcgtg cagccggtca ggccggttcc
     7261 caccaggatc aggcccgcaa agagggtcgc gagagctgtt cgatgtctca tgatgttggc
     7321 tcctattctc gcgcaaggcg aataaaacct ggttaacacc cggcggcgga atgcctatgc
     7381 tggtcgggat gggccgcccc ccggcggatg cggtcttgtc ttttccagcc gtctggggta
     7441 aacgattacc ccaagacttt acgcggaacg ggctcctgcc gatgaacaag tccatcgacg
     7501 ccatcgccgc cggccacatc tgcctcgaca tcacccccat catcccggag accggccgcg
     7561 cgcggatcga ggagatcctc acgccgggca gtctgacgat ggtcgaggcc ggcacggtcg
     7621 ccctcggcgg tcaggtcgcc aacaccggtc tggcgctgaa gcggttcggc cattcggtgg
     7681 ccgccctcgg cggcatcggc gatgatgaac tgggcctggt ggtccagcgg ctgctggaac
     7741 gccacgccgc cgccggcgcc atccggcccg cgccgggcgc ggtcacctcg ttcagcgtcg
     7801 tcctcgcgcc cccgggcatc gaccgcatgt tcctgcactg ccccggcgcc aacgacacgt
     7861 tcggcgccga gaacatcgat ttcgatctgg tccgccgggc tcgcctgctc cacttcggct
     7921 atcccacgct gatgcggcgg atgtatgccg accgtggggc cgagctggcc cggatcatgc
     7981 gccagacgaa agactgcgat gtcactacct ccctggacat gtcgatgccc gatccgcatt
     8041 cgccggccgg ccttgccgac tggccgcgca tattggaaca gacattgccc tgggtggatt
     8101 tgttcctgcc cagcgtggag gaaatcgccg tcgcgctcga acgggaggat tacctcgccc
     8161 gccgccggcg cgaccccgcc gccgatctgg tcgagcacta caccgtcgcc gacttccgcc
     8221 ggctcgccga tcgcgcgctg gcgatgggct gcggcgtctg cgtgatcaag gccggctcgc
     8281 gcgggttcta cctgcggtcg ggcccggccg agcgcctcgc ccgcttcggc ggcgccgcgc
     8341 gctgcgaggc cggtccctgg gccgaccgcg agctgtggtg ccccgcctac cggatcgacc
     8401 ggatcgcctc ggccaccggc agcggcgact gcgccatcgc gggcttgctg gctggcgtgc
     8461 tgcgcgggct cgcgcccgag cgcgccatgc gcctggcctg cgccgccggc tggcacaacc
     8521 tgcgcgcgct ggatccgctt tcgggattgc ccgactgggc gggcctggag gcgtttgtcg
     8581 attcggggcc gccggtgctc gatccgcggc tatccagtga cgaattcaac tggaagccgg
     8641 gcgcggaggt ctacgtcggc acgcgcgacg cgcgccgctg aaccgccgcc gcgcgccggc
     8701 gcggaccgct cgttccgtct ggggtgggat cgttcagaaa aggttctgag agacaatgcc
     8761 ggcgccgccc tggatcgatt tagactggct cgatcctcgt gttgcggtcc aggggggcgc
     8821 cggcgaacgc agggcgtcca ggcggacgcg ggcggggctc cggacggcga aaccccgccc
     8881 gccgcgctat tgcatgccga acgtcgaaag ctccaccggc acgaccacat tctcgaccgg
     8941 ctggagctgg ccgcggatct cgccgccggg attgttcgac gtgtggatat tgacgtagag
     9001 ctggtccatc ggccccgcca gcgcgcccag gtagtcccag acgccaagct tggggttgct
     9061 cgtcggcagc gtgtggagcg gcccggccgg gcttcccggc gcggcgtagc cgtggatgtg
     9121 cgccaccgat tccgcgctcg tcaggtcggc atacgtgatg taatagccga gctgcaggtt
     9181 ggccgggtcg tgggagaacg acgcgacgcc gtaggcgctc gagctgaccg aaccgccctc
     9241 ctgcgcggca tccaggaagg cgagattgtt ggtgatctgg ccgcggatct cgccgccggg
     9301 ataggtcgtg gagtggacgt tgacataggt caagcccgac agaatcgccg cttcctggtt
     9361 ctcgttgtaa ttccagctac cgtccttggg gctgccggcc gccagtgtgt gcagggggct
     9421 ggaggcggtc ccatgcaggg atagcccgtg gatgtgcgcc gccgactccg cggcgacgag
     9481 cccgtcgaag aggatgtagt agtccagcgt gtttgcatcg cggtcgatca tgaacagccc
     9541 gacgcccgac gcggtcgagg tattggaggg gttgtcctcc tgcgcggcgt cgagcagcgc
     9601 gaccatgtcc accacctggc cgcggatctc gcccggcgca tggttgctcg agtggacgtt
     9661 gacatagacc cggccgccca ggatgtcggc ctcctggccc tcgtcgtagt tccaggtgcc
     9721 ggttttgagc gcgccgaggg cgagggtatg gatcgggccg gcatcgacgc ccgggtcggc
     9781 cggtccgtgg aagtgagcgg cggtttccga cgaactcaag ccgccgaagc ggatctcgta
     9841 atccagggtg ttggcgtcgg tgtcgatcgt gtaatgaccg acgccgaggg cgtcgctgac
     9901 gacgccgtcc tgcgccccgg tcagcaggct gaccatcttg acgatttcgg cgcggggggt
     9961 cgcgcagggg agcagcagag ccagggtcag ggcgaacaag ggcccgcgga cgcgccggcg
    10021 ggcggaagaa ccaggtgtga aacgcatgat aatcctccat ggcgcgcgag ggggcaatgc
    10081 gcgcggaacc gaggtgaagg gatgaggatg cggcggcgaa gccgggccgc ggtcggatca
    10141 ttggagaggg agacatcatc attttcgcct tgggtggata attcgtcaac ggaatattcg
    10201 ggtcaaatta aagcaaggag gttaagatcc gaaagatcga aaagaggcgg cgtcctcgcg
    10261 cgaaaattcc gtctctcgtc ctcgtcctcg tgagcgcccc caaagggccg ctcaagatcc
    10321 ggctctcggc tcccgtcact cgtcctcggc tcgggaaact ccctcttttc ctcaagacga
    10381 gacctcgccg actccgctca cgcgccggcc tttaactgcc ctccgcggca tgctatggtg
    10441 gaagcgtgcg tcgtccgccg cggcgccgcc gacctccgct tcgaccggga tcgccccacc
    10501 gcatgccccc ccgcgtcaca atcatcgact atggcatggg caacctgcac agcgtcagca
    10561 aggcgctgga gcacctggaa tgcgcggcgg aaatcaccga tgacccggcg cgcgtccgcg
    10621 aggcgcgccg gctgatcctg cccggcgtgg gggcgttcgg cgacgcgatg cgcgagctgg
    10681 agcggcgccg gctggtggag cccctgctcg accgggtggc ggccggcgtg ccgctgctcg
    10741 gcgtctgtct ggggatgcaa ctcctgctcg agacgagcga ggaatcgccg ggcgtcgccg
    10801 gcctggggct gatcccgggc accgtgcggc ggttccgaac cgatctcaag gttccgcaga
    10861 tgggctggaa cacggtgcgt caggtgcgcc aggccccgct gttcgccggc attccagatg
    10921 aatcgtggtt ctactttgtt cactcatatt acgtcgcgcc ggccaacggc gccggcggcg
    10981 cggtcgcggg ggtgaccgat tacgcgggcg aattcccctc ggtcctgtgg cgcgatcatg
    11041 tgcaggcgac ccagttccac cccgaaaaga gccagagcct cgggcttcag atgctgagga
    11101 atttcacgcg gatggagtag acgcgccatc acgaggacga ggacgaggga cgaattctga
    11161 gggggatgga aaggctgagc gcatggccgc cacgggcgac ctcatcattc gagtacccgc
    11221 atccaccagc aatctcgggc cgggctatga ctgtctgggt ctggcgctga atctgtataa
    11281 cgacttcacg gtgcaggtga atcgccggcc gggggcgcgc agcacggtga ccggtcgcgg
    11341 ccgttgcgcc gggatccccg ccgaggataa tccgttcgtg cgggcgtatg cggccgtggg
    11401 ggaaatggcc ggcgccgacc tgcccgccgt cagcgtcacg gtcgacggcg acgtgccgct
    11461 gggcgtcggt ctgggttcgt ccgccggggg ggccgtcgcc ggcgcgctgg cggccaatgc
    11521 cctgctcggt ttgccgttca cctatgaaga actgatcgag ccgctcgtcc gggtggaggg
    11581 gcatcccgac aacgtcacgc cctcgctgct gggggggctg acggtttcaa cggcgaccga
    11641 tgccggcccg ctgacccatg tctatcaggt cgccgaagcg tggcgcctgg cgtttctgat
    11701 cccggattac gtcgtcgaga ccaccgcggc gcgcggcgcg ctccccagcc gcgtcccgct
    11761 ggcggatgcg gccttcaatc tctcgcgcgt cccgttcgtc attcacgcgc tggtggcggg
    11821 cgatggcgag atgctcggcc gcgtcatgga ggaccggctg catgagccgg tgcgagggaa
    11881 aatgatcaag cgcgacaagc ggatccggcg ggcggcgcgg gcggcgggcg cctgcgccac
    11941 gttcatcagc ggctcggggc cggccatcgg cgcgttctgc ctgggccagg aaacggccga
    12001 gcgcgtcgcc gaggcgatgc gggcggagac cgagggggcg aagttcacgg ccgaggcgct
    12061 ggtgctgcgc cccaaacgcg gcggcgcgcg catcgccgag ggcgatcaac cttagatttt
    12121 ccgcacgaat tcaaagttgc acagccggca ttcgatccgg ccgtggatgt cgcgatggaa
    12181 ccggccccgc gcgtcgcgcg acagcagcag gtggtgcgat gccagccacg gcgtgccgta
    12241 gcccgcggcc gccagtcgct ccttcatcca ctcgaccatc tcgtgcacct gcgccggttt
    12301 cagccgcccc tcgcgcacgg cctgatcggc attgagcgca tggcggtgaa tgtcggtcag
    12361 gtccgccgga acttcctccg gtccgttcca gaagccccac accttgatgt agtcgtgatc
    12421 cgggcggagg atcggctcgc cgtcgggcgt ccgcagcgcg gcgtgggagt cgaaccggcg
    12481 gcggtcggcc agatagtcgg ggttggcgga ggtggagccg gtcttgtaga tcgcgcgcgg
    12541 ctgcgtcacc ggcacgccga gccgctccaa ctccagcgcg atcgcaaact cctcgaacgg
    12601 gctgttgtaa ccctgttcgg ccagcgcggc ggcttcggga atctccagat cgggcttctc
    12661 gccgacgcgc gatgttttcc agaccagatt gatgtgatcc ttcgtgcgcg tgtagaacac
    12721 ctggtggcga ctggagagct gcacgcacgg cgtcttgcgc cagcgttcgg gctggaagta
    12781 gtcgtagagc cgcgcgtcgt ggccgacaac ccacagccgc ccccccgtgc tctcgacgac
    12841 gccatagaca taatcgacgc cgaagatcgt caccggccac agatgcgccg gcagcggcgg
    12901 cacgtccggc ccggagaagc gccgttcctg gaacagatga tactccgcgc ggcggatccg
    12961 gcggcgctcc tcttcgtatt ccggcgtgcg gcccagcaac tcgaaatcga ccagcgcata
    13021 gacgacctgc ccgcgccggc tggcgagcga gccgtcgttg cgcttgcgca cgatgatgtg
    13081 cgccggcttc atgtcggcca ccacgaaccc cttggccgcc agttcgccgc ggacgcggtg
    13141 ggtcagcgca tgcagctcct cggtcggctt gcgcagttgc tggaagacct cggccgcatc
    13201 gatgccgcgg atccactcgt agaccatgat gtaatcgcgc aggatgtcga tctccacgcc
    13261 ggggtggcgc agcagtttgc gctcgatc
//
LOCUS       DAOPCO010000084        13150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4706_length_13150_cov_4.832455, whole genome shotgun sequence.
ACCESSION   DAOPCO010000084 DAOPCO010000000
VERSION     DAOPCO010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13150
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4706_length_13150_cov_4.832455"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /gene="nadA"
                     /locus_tag="PLS90_07715"
     CDS             complement(<1..888)
                     /gene="nadA"
                     /locus_tag="PLS90_07715"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPK02330.1"
                     /translation="MPAVSPVLEHDAIDPSLDLFAEIERLKTQLNAVLLAHYYQDPDI
                     QDVADHIADSLKLAQVAAETDADVIVFAGVHFMAETAKILNPDKLVLMPDAQAGCSLA
                     DSCPPRAFAEFRAQHPDHMVISYINCSAAIKAQSDIIVTSSNAEKIVRQVPEEQPIIF
                     APDRNLGAYLNRVTGRNMLLWNGSCIVHEMFSEKKIVQLKARHPNALVLAHPECEPVV
                     LEHADHIGSTSSIREFARRTPVKELIIATEPGLIHLMQKDSPDKVFIPAPPTNTCACN
                     ECPHMKRNTLEKLYLCMRDR"
     gene            complement(1156..2589)
                     /locus_tag="PLS90_07720"
     CDS             complement(1156..2589)
                     /locus_tag="PLS90_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPK02331.1"
                     /translation="MNRPPDQSESPSRMEAGPPRAPFSLRRALAELPRRLRELEEQLW
                     LVPYETTRELRDRALRWAQILYLSLRGFFEHRAFTNASSLTYTSLLTIVPTLALAVAV
                     LKGFDLDESLFNLILSYPVAQVSPIDQALLDVRSYVANTRTGSLGLAGLLALLIAAGS
                     LLFNIEDAMNQAWSVVPGRRNFARKLSAYLNFVVILIFMIVAFSMTTSGSILTAQGIW
                     DWQIYRFRPLHYVPILILKVAPFLLIWFSLIMIYYIIPGATVQWRSAVLSALVAGVVF
                     QLAQILYIQFWAALLSKRYNVIYGAFAGVLLLLVWIYVSWCVVLWGAELCSAHQNLKD
                     WRRRRRKWHNTPFERETLALRLAVLLGRPLLARTGAARMNVGDMADLLMLPPGPITEL
                     LALFEKNRLVIRPAGEDTYLLARSPEDLPVMEVLRLVRHGGVAREAFRDGNAGEAGSP
                     VLLGPLATMTVRDLTLQPLESVQSFAI"
     gene            complement(2796..2999)
                     /locus_tag="PLS90_07725"
     CDS             complement(2796..2999)
                     /locus_tag="PLS90_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02332.1"
                     /translation="MRNVWRSNEAGSLRLRLRSGGLARRTERAGQSLEEREPEELSVP
                     VDNRPSRELVLALLQAEAAQRIN"
     gene            complement(3002..3982)
                     /locus_tag="PLS90_07730"
     CDS             complement(3002..3982)
                     /locus_tag="PLS90_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK02333.1"
                     /translation="MDFEPDLNELDVNTEPSGDVTEAAGLEGGGMLDVAGILDEGPRQ
                     SVGREAFPERSANPLRHFSLTTQPFADNVNPDYFFRTEAHEEAFLAMKRCIEEHISLG
                     LTTAISGTGKTLLTQVLIHELDPRQYQPILVLAYPGMSRSALLREIAGELKLEDVPPR
                     ANMHTLVTAIQAYIIQLYLKGIRLVLIIDEVHFLGADSLHILRTLSNIEVPERKLVTV
                     LLFGEQIFLKKLEHPNFKSVFSRMFSRVELRPLHATEVPQYVKFRWLMAGGRPTVFGD
                     EVFDRLHALTGGIPREINRLCHNVVAHAARRGEERITADLLDQLAQNGRI"
     gene            complement(4031..5218)
                     /locus_tag="PLS90_07735"
     CDS             complement(4031..5218)
                     /locus_tag="PLS90_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPK02334.1"
                     /translation="MHQRFTIAQVVQATGLEECEIRFFEQVFREFLTFSRLSLDETEF
                     TQDHIDILRRIKTLIHDEQQSIDEVKRQLKRALVSDGANGNGHANGNGAVERSRAEAP
                     LDAPPRETEGLTPYTRAASRRRLARVISVTSGKGGVGKTSVTVNLALAFARAGKKVAI
                     FDADLGLANVHILMGVKPRFNLRHLIEDNVQLEDIVTIAAEGVKVISGGQGVREMANL
                     SAEQRRVILRQMDRLEREVDILLVDTGAGISENVLRFATFSDEIIVVTTPNIAAAADA
                     YSIIKILLEMEPASKIGLIANMVQNMYHSKNVFNRINSATRKYLHYSLGDLGYIVADH
                     HLETAGQVRRPLLTEYPDCEASKCIRAIANTILNTNVFVNDRKESSFGDMMGALKRTM
                     VGV"
     gene            5841..7271
                     /locus_tag="PLS90_07740"
     CDS             5841..7271
                     /locus_tag="PLS90_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPK02335.1"
                     /translation="MSRSSDSGEPPPSRGARRRLLHFLFILLLILGGGWWLGAQPVQS
                     AEATPAVLLPLFTLFLVLLCCSGLISSSETALFSLDKVDLARLRGSTRWIDLRILRLL
                     DRPNDTLITILILNNFANIAISLTVAAITDRLLGSASAVSFLAAAVAATTTILLMGEI
                     VPKMLAHQRPLPAARVLSPALAGASWLMYPLRTFLNFCLKGLYRLLRIPTRRRPEDVT
                     DDELKAMISAGEVAQILEQDEREMIDGVFELRRTTVQEILTPRLSVTAVPDDLDQAAM
                     LDRLRQSPNNRVLVYHDSPDELVGFLLVKEVLLDAESPWRNHLREPLCVPERVGLLDL
                     LKMFRQQRTKMAVVVDEYGGVAGIVTLQDLLEEIVGDIYEKHEYVSHDIQPIGEGAWR
                     ITGSFNLEELADQLGITFPERMGVTLGGFVMNTLGRVPRVGDEVHYEGHAFRVEEMMG
                     RRILGITVRREAPAEAAAPDQTGELP"
     gene            7268..8668
                     /locus_tag="PLS90_07745"
     CDS             7268..8668
                     /locus_tag="PLS90_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPK02336.1"
                     /translation="MTLALILTAIALCMALSFYFSGAEVAIVSANRYRLRSMEEQGDA
                     SAGRLVELLEDSQRLLVMALVGTNLANVLTALFFKLFLQRGWPELAATEALGVILWSE
                     VLSLLILTPILIVFAEILPKALFRAHADALIHRLHFSLRLCLVLLKPVIWSIERVAQL
                     ILSPWSESHRATMRQLTREDVITLLSPEPAAAADSTTADAVEETIAHDAAERERHEPF
                     GEAIAREHDGEEERLSESADQRRMIQNIIELHETLAREIMTPLVDLVAVDLKRYDLNM
                     LKSLAQQSGFSRFPVYRDRIVNLIGYVDIFRVLREDDGTRKLEDFIERAHFVPETKRV
                     DDLLEEFLQMRIKNAIVVNEYGGCSGWISREDMLEEIVGELEDELDEPGDEIVEQAEG
                     VYLADGRTEIDHLNDVLGAEFDDQEWETIAGLFLSEFGHIPQVNDSVCVDGWRLTVVR
                     MDGLAIDTIRLEREKN"
     gene            complement(8734..9903)
                     /locus_tag="PLS90_07750"
     CDS             complement(8734..9903)
                     /locus_tag="PLS90_07750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02337.1"
                     /translation="MWFLVQTPHPWRGIRQRPHHLMAWFARRGHRIHWVQPRYLRWLV
                     ERRRDFLEARREEPLPGLTVQPVTLLNGERLPAVRRCNRSRLARALGAGSGGGEEAGP
                     RILWLYNPHESHLADAVPHDLLVYDIMDEYRGFPWSPPRIAEEEAELLRRADLVFAGT
                     HALHEAKRAMAGGRVECILSGVDRELFAAAGGHGKAEAARRTSSAHQAIRRRARRLAG
                     YAGMIDQRLDQSLLRAAARALPDWEFVLVGPTVGELARLRGDPRIHLHPPVRHEDLPW
                     YYRAWDAALLPFVESELTRHINPTKMLEYGAAGTPIVARALPDVARFYADGAWLYREP
                     EEFLAHLREIDLLPPDAPAPRQRAALQWAEERGWDRLADRMLAIVEEMLARRRPH"
     gene            complement(9911..11020)
                     /locus_tag="PLS90_07755"
     CDS             complement(9911..11020)
                     /locus_tag="PLS90_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPK02338.1"
                     /translation="MTPRRIDPRGRELARRIQRIQHGLRRRRLDALLVFDRHNSRYLL
                     GFASSLSYLFITPREAVAYFDGRYYEAASAAISHLEVRLMAQPRTAIERWGNEFHPRR
                     IGFEGSTAFSSLEQWRELLPGLKWEECGELIARQRIVKSPAEAKLIERSAAMADAIFE
                     TVIAALRPGMTEWDVRNLIHAEAARRGARSLSFECIVASGATGSRPHYEAQARPLERG
                     DLLLIDMGVVVEGYCSDMTRVVALGRAPRARLRKAYDAVLAAEEAALAEVAPGVACAD
                     LDRIAREHIKARRFGRYFTHGLGHGVGLEIHEPPTLNGRSKEVLRAGMVVTIEPGVYL
                     PGLGGVRIEDLVLVTRNGRRVLSRAPKAFRIVPFE"
     gene            complement(11017..12192)
                     /locus_tag="PLS90_07760"
     CDS             complement(11017..12192)
                     /locus_tag="PLS90_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK02339.1"
                     /translation="MILLGLGLGLGLGLATRLGDLPVSLDPSAAALAQIRAPARSSAG
                     GDPHGSRSARHTIVVDVARQVSPAVVSIGVTKTSYGREYQLFFDDPFFAPYLIRPRRQ
                     DFPYLGSGFIIDEAGHVVTNYHVVEGASSITVTLTDRRAFSARLLDADRFVDVALLQI
                     EGLSEREHLPTIALGDSDDIMIGETVLAVGNPFGPLIADPRPSVSVGVVSATSRSFRG
                     DQNGRIYQNMIQTDAAINPGNSGGPLLNLDAEAIGVNTFIFSRSGDSASVGFSIPINR
                     VRRIVEEILEFGRLRAIRMDFETVSLSPPIVQMLDLGVTEGALVRAIDIGGPAERAGL
                     QPKDVITRVDGIDIRDADDLMASFLTRTVGETLDMTVRRDGEELHLKYVIEEGRPPE"
     gene            complement(12358..12636)
                     /locus_tag="PLS90_07765"
     CDS             complement(12358..12636)
                     /locus_tag="PLS90_07765"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HPK02340.1"
                     /translation="MTPPARRRRFEHLPPFPGLETIIRVYHLPPPAIQYLTALTEAAD
                     GVGLVRTLDEERGIVECWVMPDFEHEFDQILAATAAQWPLQPLDDTRE"
     gene            complement(12633..13124)
                     /gene="coaD"
                     /locus_tag="PLS90_07770"
     CDS             complement(12633..13124)
                     /gene="coaD"
                     /locus_tag="PLS90_07770"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPK02341.1"
                     /translation="MYPGTFDGLTYGHLDIIRRGSKLFDRLTVAVAHNEDKNPVFTVE
                     ERIEILRTETRELPNVEIASFYGLTMDYAEKIGASYVIRGIRVVSDFEYELQMALMNR
                     ALNPRVETLFMVPAAEQLYVSSSLIKEVLLLGGDVSNFVPPSTEKLLRRRFNVPRSSP
                     GAS"
BASE COUNT         2278 a         4465 c         4174 g         2233 t
ORIGIN      
        1 gcggtcgcgc atgcacagat acagcttttc cagcgtgttg cgcttcatgt gcgggcattc
       61 attgcaggcg caggtgttgg tcggcggggc gggaatgaat accttgtccg gactgtcctt
      121 ctgcatcagg tggatcagac ccggttccgt ggcgatgatc agctccttga ccggcgtgcg
      181 gcgggcgaat tcccgaatcg acgatgtcga accgatgtga tcggcgtgct cgagcaccac
      241 cggttcgcat tccggatgcg ccagcaccag cgcattggga tggcgcgcct tgagctgcac
      301 gatcttcttc tcgctgaaca tttcgtggac gatgcacgac ccgttccaca gcagcatgtt
      361 ccgcccggtc acgcgattca gatacgcccc caggttgcga tccggggcga agatgatcgg
      421 ctgctcctcc ggaacctgac gcacgatttt ttccgcgttg ctgctcgtca caatgatgtc
      481 gctctgggcc ttgatcgcgg ccgaacagtt gatgtaggag atcaccatgt ggtcggggtg
      541 ctgcgcccgg aactcggcga acgcacgggg cggacaggaa tccgccagcg agcagcccgc
      601 ctgcgcgtcg ggcatgagca ccaacttgtc cgggttcagg atcttggccg tttcggccat
      661 gaaatgcacc ccggcgaaca caatgacgtc ggcatcggtc tcggccgcca cctgagccag
      721 cttcagcgag tcggcaatgt ggtccgcgac gtcctgaatg tcggggtcct ggtagtaatg
      781 cgccagcaga acggcattga gctgggtctt gagccgctcg atctccgcaa acagatccag
      841 cgatggatcg atggcgtcgt gttccaggac ggggctgacg gcgggcatgg cgggtcgctc
      901 ccagcggaat cgaatcgctg tcggccggtt ggccgacctt ctatcttacc atgcgcgaaa
      961 agcgcgtgag gataaggaaa ttgaagattt ccatcacctc ttgtcgtcta aattggagca
     1021 atgacgcagt cctcaacggg ggtgtgccgt gtccaagatc gtgacactca gactcccgga
     1081 tcgcgcggag cgtgctgatc ctcacgatcg atcagcgtcg cgacgcctac cgctagcgct
     1141 ggaattccca ctcccctaaa tcgcgaagct ctgcacgctt tccagcggct ggagcgtgag
     1201 atcacggacg gtcatcgtgg cgagcggacc gagcagcacg ggcgaaccgg cctcgccggc
     1261 gttcccgtcg cggaacgcct cgcgcgccac gcccccgtga cgcaccagcc gcaacacctc
     1321 catcaccggc agatcctcgg gcgagcgcgc cagcagatag gtgtcctccc ccgcggggcg
     1381 gatgacgagg cggttcttct cgaacagcgc cagcagttcc gtgatcggcc cgggcgggag
     1441 catcagcagg tcggccatat cgccgacgtt catccgcgcc gcgccggtcc gcgccagcag
     1501 cggccgcccc agcagcacgg ccagccgcag tgcgagcgtc tcgcgctcga acggcgtgtt
     1561 gtgccatttg cgccgccgcc gccgccagtc cttcaggttc tggtgcgcgc tgcagagttc
     1621 cgcgccccac aacacgacgc accatgacac atagatccac accagcagca gcagcacgcc
     1681 ggcgaacgcg ccgtaaatca cgttgtagcg ctttgagagc agcgccgccc agaactggat
     1741 gtagagaatc tgcgccagct ggaacaccac ccccgccacc agcgccgaga gcacggccga
     1801 gcgccactgc actgtcgccc ccgggatgat gtagtagatc atgatcaggc tgaaccagat
     1861 caggaggaac ggcgcgacct tgaggatcag aatcggcacg tagtgcagcg ggcggaaccg
     1921 gtagatctgc cagtcccaga tcccctgagc ggtcagaatc gagccgctgg tcgtcatcga
     1981 gaaggcaacg atcatgaaga tcaggatgac gacgaagttg agataggccg acagtttgcg
     2041 ggcgaaattg cgccggccgg ggacgaccga ccacgcctga ttcatcgcat cctcgatgtt
     2101 gaacagcagc gacccggccg cgatcagcag cgccagcaga ccggccagcc ccaggctccc
     2161 cgtccgcgtg ttggctacgt aactgcgcac gtccagcagc gcctgatcga tggggctcac
     2221 ctgggcgacc ggatagctca ggatcaggtt gaacaggctc tcatcgagat cgaagccctt
     2281 gagcacggcc acggccagag ccagcgtcgg cacaatcgtc agcaagctgg tgtaggtcag
     2341 cgaggaggcg ttggtgaaag cgcgatgttc aaaaaagccg cgcagcgaca gatagaggat
     2401 ttgcgcccag cgcagcgcgc ggtcgcgcag ctcgcgcgtc gtctcgtacg gcacgagcca
     2461 gagctgctcc tccagctcgc gcaggcggcg cggcagctcg gccagcgcgc ggcgcaacga
     2521 aaacggcgcc cgcggcggac cggcttccat ccgcgaaggc gactcggact gatcgggggg
     2581 ccggttcatc ctgagtcatc ttgccgcagc gcgtggcatc gcgccaaacg aaaaggcgac
     2641 ccgtccccgc cctatgggtc gattctcccg gtatcggctg gctgctatta ccacggcgtc
     2701 aggactttgt gaaaagtgaa accaccagcg tcgagcagcc gtgcttgccc gccgcgctag
     2761 atcaggcgtc gagggatgga cggcgatccg gccgctcaat tgatccgctg ggcggcttcg
     2821 gcctgcagca acgccagcac cagctcgcgg ctgggccggt tgtccaccgg cacgctcagc
     2881 tcctccggtt cgcgttcctc caggctttgg ccggcgcgtt ccgtccggcg ggccaggccc
     2941 ccgctgcgaa gccgcagccg caggctgccc gcttcgttgc ttctccacac gttgcgcatc
     3001 gctagatccg tccgttctgg gcaagttggt ccagcaggtc cgcggtgatg cgttcctcgc
     3061 cgcggcgggc ggcatgggcc acgacattgt gacacagccg gttgatctcg cgcggaatgc
     3121 cgccggtcag cgcatgcagc cggtcgaata cttcgtcgcc gaaaacggtg ggccggccgc
     3181 cggccatcag ccagcggaac ttcacatact gcggcacttc cgtggcatgc aacggccgca
     3241 gctccacccg gctgaacatc cggctgaaaa cggacttgaa attgggatgt tccagcttct
     3301 tcaggaaaat ctgttcgccg aacagcagca ccgtgacgag cttgcgctca ggcacctcga
     3361 tgttggacag cgtccgcagg atgtgcagcg aatcggcccc caggaaatgg acctcgtcga
     3421 tgatgaggac caggcggatg cccttcagat agagctgaat gatgtaggcc tggatcgcgg
     3481 tgacgagcgt gtgcatgttc gcccgcggcg gaacatcctc cagcttcagc tcgccggcga
     3541 tttcgcgcag cagcgccgag cgcgacatcc cgggatacgc cagcacgaga atcggctggt
     3601 actgccgcgg atcgagttcg tggatcagca cctgcgtcag cagcgtcttg cccgtgccgc
     3661 tgatcgccgt cgtcagcccc agcgaaatgt gctcctcgat gcagcgcttc atcgccagaa
     3721 aggcctcttc gtgcgcttcg gtgcgaaaga aataatcggg gttgacgttg tcggcgaacg
     3781 gctgcgtcgt cagcgagaaa tgccgcagcg ggttggccga gcgctcgggg aaagcttcgc
     3841 ggccgaccga ctggcgcggc ccttcgtcca gaatccccgc cacgtccagc atgccgccgc
     3901 cctccagccc cgccgcctcg gtcacgtcgc cgctcggctc ggtgttcaca tccagttcgt
     3961 tcaggtccgg ctcaaagtcc atcggggggc tccttccact ttgagggaaa ccgggtgggg
     4021 acgacgccgg tcagacgccg accattgtgc gcttgagcgc gcccatcatg tcgccgaagg
     4081 aggattcctt gcggtcgttc acaaagacat tggtgttgag gatcgtattg gcgatcgcgc
     4141 ggatgcactt ggacgcctcg cagtcgggat actccgtcag cagcggccgg cgcacctggc
     4201 ccgcggtttc caggtggtgg tcggcgacga tgtagcccag gtcgcccagc gaatagtgca
     4261 gatacttccg cgtggccgag ttgatccggt tgaacacgtt cttgctgtga tacatgttct
     4321 gcaccatgtt cgcgatcagc ccgattttgc tcgccggttc catctcaagc aggatcttga
     4381 tgatcgagta ggcgtcagcg gccgccgcga tgttcggcgt cgtgacgacg atgatctcgt
     4441 cgctgaacgt cgcgaagcgc agcacgttct cgctgatccc cgcgccggtg tcgaccagca
     4501 gaatatccac ctcccgttcc agtcggtcca tctggcgcag gatcacgcgg cgctgctcgg
     4561 cgctcaggtt cgccatctcg cgaaccccct ggcctccgct gatgaccttg accccctccg
     4621 ccgcgatcgt cacgatgtcc tcgagctgga cattgtcctc gatcaggtgg cgcaggttga
     4681 accgcggctt gacgcccatc aggatgtgaa cgttggccag gccgaggtcg gcgtcgaaga
     4741 tggcgacctt cttgccggcg cgcgcaaacg ccagcgccag gttgaccgtc acgctcgtct
     4801 tgccgacccc ccctttgccg ctggtgaccg agatgacgcg cgccagccgg cggcggcttg
     4861 cagcgcgtgt gtagggcgtc agcccctcgg tttcgcgcgg cggggcgtcc aggggcgcct
     4921 ccgcgcggct tcgctcaacg gctccattgc cgttggcatg accgttgccg ttggcgccgt
     4981 cgctgaccag cgcgcgcttg agctggcgct tgacctcgtc aatcgactgc tgctcgtcgt
     5041 ggatcagcgt cttgatgcgg cggagaatat cgatgtggtc ctgggtgaat tcggtttcat
     5101 ccaggctcag gcggctgaag gtaagaaact cgcgaaagac ctgttcgaag aagcggattt
     5161 cgcattcctc gagcccggtc gcctgcacaa cctgggctat cgtgaatcgc tggtgcatgg
     5221 tgagtcaatc ctccttcacc gggggcagtc gcgggggacg ggagcgcaat caacggggat
     5281 gcggtaacgg gaacagagca acattacgca tgacaacggt taagacgtga acactccggg
     5341 tgttcttccg gcgggccggg cctttcatga gctttcaaag aacgcggcgc tcgcggcgtc
     5401 aagccgctga catgacatcg ttaggctcca cagctagcag accccgcggc ccgacgcaag
     5461 cataatccta cttccggcaa ccctccaggc ggtgctttcc ccccgtcgtg atccctgttt
     5521 atcccttcgt gactcacaca tcccgcccaa ttcgtgcggg aaaggcttgc gcgtcccggg
     5581 ccgccccgtc tagactactc ccatgcggcc tcaatggtgc gagggatccc gaatcgtgct
     5641 cgcggatcga cgcgcgccgc cgcctctcag cttgagacca acgacccacg accccacgcg
     5701 gcccccacgc gccggcgtcc aagcagcctt gatctgaacc ggctcacccg acttttcgat
     5761 gcgccccccc ttgaatcttc cttcagctct cagtcaacag gtggtcccca ccgccacggc
     5821 ggccgaccag gagttggatt gtgtcgcgtt catccgatag cggcgagcct cctccatctc
     5881 gcggggcgcg gcgccggctg cttcatttcc tgttcatcct gttgctgatc ctgggcggcg
     5941 gctggtggct cggcgcccag ccggttcagt cggccgaagc gacccccgcc gtcctgctgc
     6001 ccctcttcac gctcttcctg gtcctgctgt gctgctccgg cctgatctcg tcgtccgaaa
     6061 cagcgctttt ctcgctggac aaggtcgacc tggcgcgcct gcgcgggtcg acccgctgga
     6121 tcgatctgcg catcctgcgg ttgctcgatc gccccaacga cacgctgatc acgatcctga
     6181 tcctgaacaa tttcgccaac attgcgatct cgctgacggt cgccgcgatt accgaccgcc
     6241 tgctcggatc ggcgtccgcc gtctcctttc tcgccgccgc cgtcgccgcc accacgacga
     6301 tcctgctgat gggcgagatc gtgcccaaga tgctcgccca ccagcggccg cttcccgccg
     6361 cccgcgtcct ctcacccgcc ctggccggcg ccagctggct gatgtatccc ttgcgaacgt
     6421 tcctcaattt ctgcctcaag gggctctacc gcctgctgcg catccccacc cgccgccgcc
     6481 ccgaggatgt caccgacgac gaactgaagg cgatgatcag cgccggcgaa gtggcccaga
     6541 tcctggagca ggatgaacgg gagatgatcg acggcgtctt tgaactgcgc cgcaccaccg
     6601 tccaggagat cctcaccccg cgcctgtcgg tcacggccgt ccccgacgac ctggatcagg
     6661 ccgcgatgct cgaccggctg cgccagagcc ccaataatcg cgtcctcgtc tatcatgatt
     6721 ccccggacga gctggtcggc ttcctgctcg tcaaggaggt gctcctcgac gccgagagcc
     6781 cctggcgcaa ccatctgcgc gaaccgctct gcgttcccga acgcgtgggc ctgctcgatc
     6841 tgctcaagat gttccgccag cagcggacga agatggccgt cgtcgtcgac gaatatggcg
     6901 gggtcgccgg catcgtcacg ctgcaggacc tgctcgagga gatcgtcggc gacatctatg
     6961 agaaacacga atacgtctcc cacgatatcc agcccatcgg cgagggcgcc tggcgcatca
     7021 ccgggtcctt caacctggag gaactcgccg atcagcttgg catcaccttc ccggaacgga
     7081 tgggcgtgac ccttggcggg tttgtcatga acacgctcgg ccgggtgccg cgcgtcggcg
     7141 atgaggttca ttacgagggt catgccttcc gcgtcgagga gatgatgggc cgccgcatcc
     7201 tcggcatcac cgtccgccgc gaggcccccg ccgaggccgc cgcacccgat cagacggggg
     7261 aacttccatg accctcgccc tgatcctgac cgccatcgcc ctgtgcatgg ccctgtcgtt
     7321 ctacttctca ggcgccgagg ttgccatcgt ctcggccaac cgctaccgcc tgcgcagcat
     7381 ggaggagcag ggcgacgcca gcgccggacg cctcgtcgag ctgctcgagg acagccagcg
     7441 cctgctcgtg atggcgctgg tcggcacaaa cctggccaat gtcctcaccg ccctgttctt
     7501 taaactcttc ctccagcgcg gctggcccga gctggccgcc accgaggcgc tgggcgtcat
     7561 cctctggagc gaggtcctca gcctgctgat cctgaccccg atcctgatcg tcttcgccga
     7621 aatcctgccc aaggcgctct tccgcgccca tgccgatgcg ctcatccacc ggctgcactt
     7681 ctcgctgcgg ctctgcctgg tcctgctcaa accggtcatc tggtcgatcg agcgggtggc
     7741 ccagttgatc ctgtcgccct ggtccgaatc acaccgcgcg accatgcggc agttgacccg
     7801 cgaggacgtg atcacgctcc tttcgcccga accggccgcg gcggcggaca gcaccacggc
     7861 cgacgcggtc gaggaaacca tcgcccatga cgcggccgag cgcgaacggc acgaaccctt
     7921 cggcgaggcg atcgcccggg agcacgatgg cgaggaggaa cggctcagcg aatccgccga
     7981 tcagcgccgg atgatccaga acatcatcga actgcacgaa accctcgccc gcgagatcat
     8041 gaccccgctg gttgacctcg tcgcggtcga tttgaagcgc tatgacctga acatgctcaa
     8101 atcgctggcc cagcagtccg gcttctcccg cttccccgtc tatcgcgacc gcatcgtcaa
     8161 cctgatcggc tacgtcgata tcttccgcgt cctgcgcgag gacgacggca ccaggaaact
     8221 ggaggatttc atcgagcggg cgcatttcgt ccccgaaacc aaacgcgtcg acgacctgct
     8281 ggaggaattc ctccagatgc gcatcaagaa tgccatcgtc gtcaacgaat atggcggctg
     8341 ttccggttgg atcagccgcg aggacatgct tgaggagatc gtcggcgagc tggaggacga
     8401 actcgacgag cccggcgacg aaatcgtcga gcaggccgag ggggtctacc tggccgatgg
     8461 ccgcaccgaa attgatcacc tcaacgacgt cctcggcgcc gagttcgacg atcaggaatg
     8521 ggagaccatc gccggtctgt tcctcagcga attcggtcac atcccgcagg tgaatgactc
     8581 cgtctgcgtc gacggctggc gcctgaccgt cgtgcgcatg gatggccttg cgatcgatac
     8641 catccggctg gaacgcgaga aaaactgacg cgaatccgta tttcggcccg ctgtctggaa
     8701 ctcgtccctc gtccctcacg gacgtgcctt cgatcagtgg ggccgacgcc gagccagcat
     8761 ttcctcgaca atcgccagca tccggtcggc caggcgatcc cagccgcgtt cctcggccca
     8821 ctgcagggcc gcccgctggc gtggagcggg cgcatccggc ggaaggagat cgatctcacg
     8881 cagatgggcg agaaactcct ccggctcgcg gtagagccac gcgccgtcgg catagaaccg
     8941 cgcgacgtcg ggcagcgccc gcgccacgat gggcgtcccc gccgcgccgt attcgagcat
     9001 cttcgtcggg ttgatgtgcc gcgtcagctc gctctcgacg aacggcagca gcgccgcgtc
     9061 ccaggcgcgg tagtaccagg gcagatcttc atggcgaacc ggcggatgca gatggatgcg
     9121 cgggtcgccg cggaggcggg cgagctcgcc gacggtgggg ccgaccagaa cgaactccca
     9181 gtcgggcagc gcgcgcgccg ccgcccgcag gagcgactga tcgaggcgct ggtcgatcat
     9241 gccggcataa ccggcaagcc ggcgggcgcg gcgccggatc gcctggtgcg cggacgacgt
     9301 gcgccgggcg gcttcggcct ttccatgtcc gcccgccgcc gcgaaaagct cccgatcgac
     9361 gccgctcagg atgcactcca cccgcccgcc ggccatggcg cgcttggcct cgtggagcgc
     9421 gtgcgtgccg gcaaagacga gatcggcgcg gcgcaagagc tccgcctcct cttcggcgat
     9481 ccgcggcggc gaccagggaa aaccccggta ttcatccatg atgtcgtaga ccagcagatc
     9541 gtgcggaacg gcgtcggcca gatgactctc gtgcgggtta taaagccaga ggatgcgcgg
     9601 cccggcctcc tcccctcccc ccgagccggc gccgagcgcg cgcgcgagcc ggcttcggtt
     9661 gcatctccgg acggcaggca gccgttcgcc gttcagcagc gtgacgggct gaaccgtcag
     9721 cccgggcagc ggttcctcgc ggcgagcctc aagaaaatcg cggcggcgct cgacgagcca
     9781 gcgcaggtat cgcggctgaa cccagtggat gcggtgcccg cgccgggcaa accacgccat
     9841 caggtggtgc ggtcgctggc gtatgccccg ccaggggtgg ggcgtctgga cgagaaacca
     9901 catccagcgc tcactcgaac ggaacgattc ggaatgcctt cggcgcgcgc gacaggacgc
     9961 ggcggccgtt acgcgtcacc agcaccaggt cctcgatgcg gacgcccccc aggccgggca
    10021 gatacacgcc cggctcaatc gtgacgacca tgccggcccg cagcacctcc ttgctgcggc
    10081 cgttgagcgt cgggggctcg tggatctcaa ggccgacacc gtgaccgagg ccatgagtga
    10141 aatagcggcc gaaacggcgc gccttgatgt gctcgcgcgc gatgcgatcc aggtcggcgc
    10201 aggcgacgcc gggcgcgact tcggccagcg ccgcttcctc ggcggccagg acggcatcgt
    10261 acgccttgcg cagccgcgcc cgcggcgcgc gacccagcgc caccacgcgc gtcatgtcgg
    10321 agcaataccc ctccaccacc acgcccatgt cgatcagcag caggtcgccc cgctcgagcg
    10381 gccgcgcctg cgcctcgtag tgcggacgcg agccggtggc gccgctggcg acgatgcact
    10441 caaaggaaag cgaacgcgcg ccgcggcggg cggcctcggc gtggatcaga ttgcgcacgt
    10501 cccactcggt catgccgggg cgcagcgcgg caatcacggt ctcgaagatg gcatccgcca
    10561 tcgcggcgct gcgttcgatc agcttcgcct cggcgggcga cttcacgatc cgctgccgcg
    10621 cgatcagctc gccgcattcc tcccacttca ggccggggag caactcgcgc cactgctcca
    10681 gggaactgaa cgcggtggag ccttcgaacc cgatccgccg gggatggaat tcgttccccc
    10741 agcgctcgat cgcggtgcgc ggctgcgcca tcagccgcac ctccaggtgg ctgatggcgg
    10801 ccgaggcggc ctcgtaatac cggccgtcga aataggcgac ggcctcgcgc ggcgtgatga
    10861 acagatacga cagcgacgag gcgaagccga gcaggtagcg cgaattgtga cggtcgaaca
    10921 ccagcagggc atcgagccgc cggcggcgca gcccgtgctg gatgcgttgg atccgccggg
    10981 ccagttcgcg gccgcgcggg tcgatgcgcc tgggggtcat tcggggggcc gtccttcctc
    11041 gatcacgtat ttcagatgga gttcctcgcc gtcgcgccgg acggtcatat ccagcgtctc
    11101 gcccaccgtg cgcgtcagga acgaggccat caggtcgtcg gcgtcgcgaa tgtcaatgcc
    11161 gtcgacgcgc gtgatgacgt ccttcggctg cagccccgcc cgctcggcgg ggccgccgat
    11221 atcaatggcg cgcaccagcg cgccttcagt gacgcccagg tcgagcatct gcacgatcgg
    11281 cgggctcagg ctcaccgtct cgaagtccat gcggatggcg cgcagccggc cgaattccag
    11341 aatctcctcg acgatccggc ggacgcgatt gatcgggatg gagaagccga cgctggccga
    11401 atcgcccgac cgcgaaaaga tgaatgtgtt gacgccgatt gcctcggcat ccagattgag
    11461 cagcggcccc ccgctgttgc ccggattgat cgccgcgtcg gtctggatca tgttctgata
    11521 gatccgcccg ttctggtcgc cgcggaacga tcggctggtg gcgctgacga cgccgaccga
    11581 taccgaaggc cggggatcgg cgatcagcgg cccgaacgga ttgccgaccg ccagcaccgt
    11641 ctcgccgatc atgatgtcgt cggaatcccc caacgcaatc gtcggcagat gttcgcgttc
    11701 gctcagcccc tcgatctgca gcagcgcgac atcgacaaac cgatcggcat cgagcaaccg
    11761 cgcggaaaac gcgcgccggt cggtcagcgt caccgtgatg ctgctggccc cctccacgac
    11821 atggtagttg gtgacgacat ggcccgcctc atcgatgatg aaaccggaac caaggtaggg
    11881 gaagtcctgc cgccggggcc ggatcagata cggcgcgaag aacggatcat cgaagaaaag
    11941 ctggtactcg cggccatagg acgttttggt caccccgatg ctgacgacgg cggggctgac
    12001 ctggcgggcg acgtcgacga cgatcgtatg ccgcgccgat cgggagccat ggggatcgcc
    12061 gccggcgctc gagcgggcgg gagcgcggat ctgcgccagc gcggcggccg acggatcgag
    12121 cgacaccggc aggtcgccga ggcgggtggc gaggcccagg cccagtccaa ggcccagacc
    12181 caacagaatc atgcttgcga gaaccaggag gatgggaggg gaacgacgca tggcggcctc
    12241 cgggcgcgaa atcctcagcc gttttattat aacgcggggg catcgcggcg caagatgcgg
    12301 caacgcgccg cacggcgggc cttcccgtcg caccctcaca cgcccctcgt caagggctca
    12361 ctcgcgcgtg tcgtccaggg gctgcagcgg ccactgcgcc gccgtggccg ccaggatctg
    12421 gtcgaattcg tgctcgaagt ccggcatgac ccagcactcg acgatgccgc gctcctcgtc
    12481 cagcgtccgc acgagcccga ccccgtcggc cgcctcggtc agcgccgtca gatactggat
    12541 ggcgggcggc ggcaggtggt agacgcggat gatcgtctcc aaccccggga agggaggcag
    12601 atgctcaaac cgccggcgcc gggcgggcgg cgtcatgagg ccccgggact ggaacgcggg
    12661 acattgaacc ggcgccgaag gagtttttcg gtcgacggcg gaacaaagtt cgacacgtcg
    12721 ccccccagca gcaggacttc cttgatcagc gagctgctta catacaactg ttcggccgcg
    12781 ggcaccatga acagcgtctc cacgcgcgga ttgagcgccc ggttcatcag cgccatctgc
    12841 agctcatact caaaatccga gaccacccgg atgccgcgga tcacgtagct ggcgccgatc
    12901 ttttcggcgt aatccatcgt cagcccgtag aacgaggcga tctccacgtt gggcagctcc
    12961 cgcgtctcgg tccgcagaat ttcaatccgc tcctcgaccg tgaagaccgg gttcttgtcc
    13021 tcgttgtgcg cgacggcgac cgtcagccgg tcgaacaatt tcgatccccg ccggatgata
    13081 tcgagatgac catacgtcaa tccatcgaac gtgcccggat acagcgcacg gtgttccttg
    13141 tcggccgaca
//
LOCUS       DAOPCO010000085        13149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4707_length_13149_cov_4.954330, whole genome shotgun sequence.
ACCESSION   DAOPCO010000085 DAOPCO010000000
VERSION     DAOPCO010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13149
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4707_length_13149_cov_4.954330"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLS90_07775"
     CDS             <1..800
                     /locus_tag="PLS90_07775"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HPK02342.1"
                     /translation="RYYQPLFQHVFDEQLPDAEEVIEQIWQIEPLIRPMLCDSVSCIN
                     DALAEGKRVLAEGAQGVLLDIDFGTYPFVTSSNPSTGGVCTGLGVPPRAITRVMGVVK
                     AYTTRVGEGPFPTELDNALGEKLRADGGEFGATTGRPRRCGWFDACVVRRAVQVCGVE
                     HVALTKLDVLDGLEEIQICTHYETPDGRKLERMPLELTQLEACRPVFETLPGWQTSIA
                     DCTEYRELPGPAQAYVARIEQLLGVHIPIVSVGAKRKLTILRQASFF"
     gene            839..1561
                     /locus_tag="PLS90_07780"
     CDS             839..1561
                     /locus_tag="PLS90_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HPK02343.1"
                     /translation="MPSSAARSPIAMGLYNRMRAAAGHRGWWPADRSAPDRGRDEIII
                     GAVLTQNTSWKNVERAIESLRAAGLLSLARLAAMEPDQIAPHIRAAGYFNVKARRLHS
                     VARFFAPGGKPRFTQLARLGDDELRAQLLAVHGVGRETADSILLYALGRLSFVVDAYT
                     LRIGVRHGLFPPGADYEQARAWFTTRLPHDAALFNDYHAQLVRIGNLYCKPRPRCAAC
                     PLARRDCTASREAWAAIRERLA"
     gene            1646..2716
                     /locus_tag="PLS90_07785"
     CDS             1646..2716
                     /locus_tag="PLS90_07785"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HPK02344.1"
                     /translation="MRDAPFNPADHPHRRRNALTGEWVLVSPHRAQRPWRGGRETPPA
                     DSPPAYDPACYLCPGNERAGGARNPHYADTFVFTNDYTALLPEAPAAGAPDHPLLTLE
                     TVRGVCRVICFSPRHDLTLAQMDQAAVRRVVDVWIDQTLELGRQWRWVQVFENKGALM
                     GCSNPHPHGQIWAGDALPNEPAREDAEQRRYFTDRGRPLLLDYAQLELDREERVIVQN
                     EDWLAVVPFWAVWPFELLLLPRRRQIRRLPELSDSERGALAEILRRLLIRYDNLFQID
                     FPYSMGWHGAPFGDDGDEATAHWQLHAHFYPPLLRSATLRKFLVGYEMLAEVQRDITP
                     EQAANRLRAAAEIHFAASPSDR"
     gene            2713..3900
                     /gene="galK"
                     /locus_tag="PLS90_07790"
     CDS             2713..3900
                     /gene="galK"
                     /locus_tag="PLS90_07790"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPK02345.1"
                     /translation="MTSQPLPPASPLPGQVVRMFRDRFHTEPAFLVRAPGRVNLIGEH
                     TDYNGGFVLPMAIERAVWIALRPRADELVTVWSLDFHEEQSFSAVAPRHDPSAGWVEY
                     QKGVAWALGAEGVPLRGWEGVVGGDIPIGGGLSSSAALELAAARAFAAVANLDWDPIR
                     MARLARQAENHWVGVSCGIMDQLISAAGRRDHALLIDCRTLAISPVPLPAGAAVVILD
                     TATRRSLESSAYNERRARCEAAARHFGLDSLRDLTREMLEARGAELEPVTRRRARHVV
                     TENARTLEAAEALRAGDATRLGRLMDASHASLRGDFEVSSPELDQMVALARARPECLG
                     ARMTGAGFGGCAIALIDAARSREFSAAVDAAYRRASGREPTIDLTRAAPGADLVPLIP
                     PSP"
     gene            4112..4585
                     /locus_tag="PLS90_07795"
     CDS             4112..4585
                     /locus_tag="PLS90_07795"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HPK02346.1"
                     /translation="MNAPHPYLFTYGTLRPGSRQIMSRVLTCHARHLGPATVAGSLFD
                     LGEFPGAVPPLHEDDSIRGDLFYLNHPRVLLELLDNYEGCGERDAPPHLFRRELAPAR
                     LADGTSYMAWVYWYGGDVAHCTQIPGGDYLALLAREELAPAGIDPGVLINLDGAI"
     gene            complement(4608..4904)
                     /locus_tag="PLS90_07800"
     CDS             complement(4608..4904)
                     /locus_tag="PLS90_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02347.1"
                     /translation="MKSIETRTYTNVVLTVLAMLLLVLVAQPYVSLPAAHAQLDLSRN
                     EQQSDKLNSVEPLADQAAATREVAAANLKIAEAITKAAAAQSEIAAALARLSVD"
     gene            5114..5965
                     /locus_tag="PLS90_07805"
     CDS             5114..5965
                     /locus_tag="PLS90_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK02348.1"
                     /translation="MTPVPSPDPRAAAAYWNQILDPRNLAHAPAAETPRAIQRDIALA
                     RTPDFLAALRHLAPTASRPPGVIDLGAGLGNHALAAARMGHRVLALDASLDRMRRLRI
                     RAARVGVQLPAVVARAEALPFRTGSVGALYTKSVLIHTDLDRALDEIARILGPGGRCA
                     LIEPQPGNPFVRLYRATLAPSAWKSITRYFDAPRQRLVAQRIGPTRLRPFYFLGFLGF
                     IFEFGVPWPKVRNGVLACLRPVDALLFSLIPLLRRWAWFGLLEAEKPAAKLNAEADSD
                     GDLSSTD"
     gene            6269..8788
                     /locus_tag="PLS90_07810"
     CDS             6269..8788
                     /locus_tag="PLS90_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02349.1"
                     /translation="MRFHRPLALLLFALPCWAAAQPNPSASAPTSAPQLLDVRLRPLG
                     DSLRIIFPLTNQAAYSVKPELASRRVRIDFIGAEIRFLPPELNRVGLPWLDGIWLASS
                     PPGIATLELRLPSPRMRITHFALTDPPAVVIDLFREDQPWVHTTVASDGPLPAVRLPA
                     SDEDRSSSASASLGNSPVAQEPVNEIRLIDADYDYFPISQHEFRSAEANEVLDDFLAR
                     RWAHVVRKGIEYLKTKPINPDETGFLLHLVAEARWRLGRDTFPQPLDEMRNYYEQALR
                     VAGAGDLGAFAHWRLAQLAGQMHDFDRALQHLRQAAATSQPAQQLRVELLRVQALRAA
                     RKTNPALKQIQALLAGAALPNGVELSNEQRVELLLDRGDILTETGQFEAAWQAYQEAR
                     KIDPAWLSLNPDSGELMALAAAEIGELTTARQTLEYINQHFSYREDERKVALALQFAD
                     VLERLGDLDAAEGTYAALLAELGDTPKGAAVERRMLKVFPDRVVGGETRYCRLLWRRG
                     KLTQAMTELDRVWNLCLREGISTEPLRELVGKIVPDFIAFCARHDQPFAAVQAWNLYQ
                     ASIGNSAARQSCMPPLIESLALLNLNDEALRQLLLYEQHATADAEDSAHLALLKAQLL
                     SRLERTVEAIELIEKNLTRIDDPDQSAKALRLLAEAYSAEDRPLEAAQAWQALAEVNP
                     ADSTTVGEASLRAGEIFLEQAMPLQAMEIGLKALFHEQQHLDQGLPVTWAPEVRDGLL
                     LMLAKAYRARGDQQRALILLDEYLARPSITAGEAAFARRMVAECHREAGRLDASRAAY
                     EAILADERAPAPWPQIARRALDELSWNEQNPQWSIPRPQRN"
     gene            8791..10077
                     /locus_tag="PLS90_07815"
     CDS             8791..10077
                     /locus_tag="PLS90_07815"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02350.1"
                     /translation="MAARLTQTTPAPPAPHDPSTSAPTSLRMRQAMQMLRGAEHKIAD
                     AYQQLGAEVQRLTAELDSSNLELRRNLAEREKMQAILISTLQSLTIGVLAVGQDGVVI
                     VANPAACEIFRRPLEQITTRHLEEILDGIPDSGQLLLTLQGAGGGQSRIAWSQPVDQG
                     APRQIELTAVRSLPPSDIHLAGLILAEDQTHLRRLEHQATLRSRLTGMGEIAMNLAHE
                     IRNPLGSIALFATALERELEGNESLAPMARQIVAGVKSLEHLVANTLEFARPRRMAMA
                     RVNLKDVIRDTLVYLEHPLEQHAIQVQYDHELQPDAWVSADAEQLRQVFLNLGLNAIQ
                     AMDDGGKLEFLIQADAAGGWEVAVRDNGVGIPRELIERIFDPFFTTRDKGSGIGLAIV
                     HSILQAHDARIEVESRVAVGTTFRVVFPAYRQFEGI"
     gene            10095..11528
                     /locus_tag="PLS90_07820"
     CDS             10095..11528
                     /locus_tag="PLS90_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK02351.1"
                     /translation="MRTGTDPNGDVAAPRGVILVADDEPGMRSALREVLRRSGWRVEA
                     VECGAQALARLAEDDAVDLLLTDFRMPGMSGLELLREARAQRPALPVIMMTAFGTVED
                     AVAAMRDGAADYLMKPFSFDTVTQTVERVMAERLDPPRTPTGRPAESATPVLPAARGG
                     NIHARIIAESPSLRALLEIAADVAEADSTVMLSGESGTGKEVVARFIHECSNRKGPFV
                     AINCAALPEGLLESELFGHEKGAFTGAVLARKGRFEQANGGTLLLDEISEMPLALQAK
                     LLRVLQEREITPIGANETIRLDVRVIATTNRDLRQEVMEHRFRQDLYYRLNVIALDIP
                     PLRNRREDILPLAEYFLARYRRKNRPAQRLGADVRRYLLDCEWPGNVRELENLIERAC
                     LLARGTEITLADLHLGPARQSTPAGAMPFPFGDDCLTLEELERRLITHTLERTGGNRT
                     RTADLLGVSVRTIRNKIHQYGLVEAAG"
     gene            complement(11880..13073)
                     /locus_tag="PLS90_07825"
     CDS             complement(11880..13073)
                     /locus_tag="PLS90_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK02352.1"
                     /translation="MAEVRKVATRRETRPRVAEKVRTLPPSGIRAFFELVIGRDDVIS
                     LGVGEPDFVTPWHIRETALYRISQGDTTYTSNSGLLSTRAAIARYLHERFKATFDPED
                     EMLLTVGVSEGLDLVLRAILEPGDEVIIWQPSYVAYAPLVTLAGGVPVALNTSIEAGF
                     GLDLNALENLITDRTRAVFINYPSNPTGATLRREDLEGLVRICVPRQILMISDEVYAE
                     LTHDGDHVSLAAIPGAAEWTVLLSGFSKAFAMTGWRLGYAAGPREVIAAMTKIHQYSI
                     MCAPIMAQRAGEAALTEGLDDMREMCAIYRQRRNMIVKGLNEIGLPCHRPAGAFYAFP
                     SIRHTGLDSETFCRKLLEEESVAIVPGNAFGEAGEGYVRMAYAASFQTIERALAGLER
                     FLKRL"
BASE COUNT         2103 a         4924 c         3936 g         2186 t
ORIGIN      
        1 cgcgctacta ccagcccctg tttcagcacg ttttcgacga acaactgccc gacgccgagg
       61 aagtcatcga acagatctgg cagatcgagc cgctcatccg cccgatgctc tgcgattcgg
      121 tctcctgcat caacgatgcg ctcgccgagg gcaaacgcgt gctcgccgag ggggcccagg
      181 gcgtcctgct tgatatcgac ttcggcacct acccgttcgt caccagttcc aatccgtcga
      241 cggggggcgt ctgcaccggc ctcggcgtcc ccccccgcgc gatcacccgc gtgatgggcg
      301 tcgtcaaggc ctacaccacg cgcgtcggcg aggggccgtt cccgaccgaa ctggacaacg
      361 ccctgggcga gaagctccgc gccgacggcg gcgaattcgg cgccaccacc ggccggcccc
      421 gccgctgcgg ctggttcgat gcctgcgtcg tccgtcgcgc cgtccaggtc tgcggcgtcg
      481 agcatgtcgc gctcaccaag ctcgatgtcc tcgatggcct ggaggaaatc cagatctgca
      541 cccattacga gacccccgac gggcgcaaac tcgagcgcat gccgctcgaa ttgacccagc
      601 tcgaagcctg ccgcccggtg tttgaaacac tccccggttg gcaaacgtcc atcgccgact
      661 gcacggaata ccgcgagctg cccgggccgg cgcaagctta cgtcgcgcgc atcgagcaac
      721 tgctcggcgt tcacatcccg atcgtctcgg tgggggccaa gcgcaagctc accatcctgc
      781 gccaggcgtc cttcttctga tccgccgcga aggcggtcca caagacacac gggagccgat
      841 gccgtcgtcc gccgccagat cccccatcgc gatgggcctc tacaatcgca tgcgggccgc
      901 cgccggccat cgcggctggt ggcccgccga tcgctccgcc cccgatcgcg gccgcgatga
      961 aatcatcatt ggcgccgtcc tcacccagaa cacctcctgg aagaacgtcg aacgagcgat
     1021 tgaaagcttg cgcgcggccg gactgctgtc gctcgcccgg ctcgccgcca tggaaccgga
     1081 tcagatcgcg ccgcatatcc gcgcggcggg ctatttcaac gtgaaggctc gccggctcca
     1141 ctccgtcgcg cggttcttcg cgcccggcgg caaaccccgc ttcacccaac tggcccggct
     1201 gggcgacgac gaactccgcg cgcaactgct cgccgtccac ggcgtcggcc gcgagaccgc
     1261 cgacagtatc ctgctttacg cgctcggtcg cctcagtttt gtcgtcgacg cttacacgct
     1321 gcggatcggc gtgcgccacg gtctcttccc ccccggcgcc gactacgagc aggcgcgcgc
     1381 ctggttcacg acgcgcctcc cccacgacgc cgccctgttc aacgactatc acgcgcagct
     1441 ggtccgcatc ggcaatctct actgcaaacc caggccgcgc tgcgccgctt gcccgctcgc
     1501 ccggcgcgac tgcaccgcat cgcgcgaggc ctgggccgcc atccgcgaac gtctggcgta
     1561 acgcttgcgg ccagaagcga agccagttgc ccggcagcgg gcttgctgtc acaatgcggg
     1621 ccgagttcac gcgtcggatc agcgcatgcg cgacgccccg ttcaaccccg ccgaccatcc
     1681 tcaccgccgc cgcaacgccc tgactggcga atgggttctc gtctcgccgc accgcgccca
     1741 gcgcccctgg cgcggcggcc gcgaaacgcc ccccgccgac tcgccccccg cttacgaccc
     1801 cgcctgctac ctctgccccg gcaacgaacg cgccggcggc gcacggaacc cgcactacgc
     1861 cgatacgttc gtcttcacca atgattacac ggccctgctg cccgaagccc ccgccgcggg
     1921 cgccccggac cacccgctgc tgaccctgga aaccgtccgc ggcgtttgtc gcgtgatctg
     1981 cttctcgccg cgccacgacc tgacgctggc gcagatggac caggccgccg tgcgccgcgt
     2041 cgtcgacgtc tggatcgatc agacgcttga gctgggccgc caatggcgct gggtccaggt
     2101 cttcgaaaac aagggcgcgc tgatgggttg ctccaacccg catccccacg gccagatctg
     2161 ggccggcgac gccctcccaa acgagcccgc cagagaagac gcggagcagc gccgctactt
     2221 caccgaccgg ggccgtccgc tcctgctgga ctacgcccag ctcgagctcg accgcgagga
     2281 gcgcgtcatc gtccagaacg aggactggct cgccgtcgtc ccgttctggg cggtctggcc
     2341 attcgaactc ctcctgctgc cgcgccggcg ccagatccgc cgccttccgg aactgtcgga
     2401 ctccgaacgc ggcgccctcg ccgagatcct ccgccgcctc ttgatccgct acgacaacct
     2461 cttccagatc gactttccct attccatggg ctggcacggc gccccgtttg gcgacgacgg
     2521 cgacgaggcc acggcccatt ggcagcttca cgcgcacttc tacccgccgc tcctgcgatc
     2581 cgccacgctc cgtaaattcc tggtcggcta cgaaatgctg gccgaggttc agcgcgatat
     2641 cacgcccgag caggccgcca accggttgcg cgcggccgcc gagattcatt tcgcggcgag
     2701 cccgtccgac cgatgacctc ccagccgctg ccgccagcca gcccgctccc cgggcaggtc
     2761 gtgagaatgt ttcgcgaccg gttccacaca gaaccggcat tccttgtccg cgcgcccggc
     2821 cgcgtcaacc tcatcggcga gcataccgac tacaacggcg gcttcgtgct gccgatggcg
     2881 atcgagcgcg cggtgtggat cgccctgcgc ccgcgcgccg acgagctcgt caccgtctgg
     2941 tcgctggatt tccacgagga acaatcgttc tcggcggtcg cgccccggca cgacccctcc
     3001 gccggctggg tggaatatca gaaaggcgtc gcctgggcgc ttggcgccga gggcgtcccg
     3061 cttcgcggct gggagggcgt cgtcggcgga gatatcccca ttggcggggg cctctcctcc
     3121 tccgccgcgc tcgaactggc cgccgcccgg gccttcgccg ccgtcgccaa tctcgattgg
     3181 gacccgatcc gcatggcccg cctggcgcgg caggcggaaa accactgggt cggcgtgagc
     3241 tgcggcatca tggatcagct catttcggcc gccggccgcc gggaccatgc cctgctgatc
     3301 gattgccgga cgctggccat atcgcccgtc ccccttcccg ccggcgcggc cgtcgtgatc
     3361 ctggacaccg ccacccgccg gagcctcgag agctcggctt acaacgagcg ccgcgcgcgg
     3421 tgtgaggccg ccgcccgcca cttcgggctc gactccctgc gcgacctcac gcgcgagatg
     3481 ctcgaagcgc gcggcgccga actcgagccc gtcacccgcc gccgcgcccg ccacgtcgtg
     3541 acggaaaatg ccagaaccct cgaagccgcc gaagccctgc gcgcgggcga cgcgacccgc
     3601 ctcggccggt tgatggatgc cagccacgcc agcctccgcg gcgacttcga agtctccagc
     3661 ccggaacttg accagatggt cgctctggcc cgcgcccggc ccgaatgcct tggcgcccgg
     3721 atgaccggcg ctggctttgg cggctgcgcg atcgcgctga tcgatgccgc gcggtcgcgc
     3781 gagttctccg ccgctgttga cgcggcctac cgccgggcaa gcggccgcga gccgacaatt
     3841 gacctcacgc gcgccgcgcc cggcgccgac ctcgtccccc tgataccgcc gagtccttga
     3901 cgatcagttt atctttttca gtaacgggtt ttggttgaca agccgggccc cttgtcgcac
     3961 aatgccttcc gagcggtcgg cacaagcttt cttctcttca gcgtcgtttc gttgcggact
     4021 gatctggacc acgctcaaac ctatcgggag aggccccacc ccgcccggtg cgagacgcgt
     4081 cccgcgcgcc gtctgctggg gccgctcggc catgaatgcc ccgcacccct acctcttcac
     4141 ctatggcacg ctgcgacccg gctcgcggca gatcatgtcg cgcgtgctga cctgccatgc
     4201 ccgccacctc ggacccgcca cggtggccgg ttcgctcttt gacctgggcg aatttcccgg
     4261 cgccgtcccc ccgctgcacg aggacgactc catccgcggc gacctgtttt acctcaacca
     4321 cccgcgagtt ctgctggagt tgctcgataa ttacgaaggc tgtggcgagc gcgacgcccc
     4381 gccgcacctc ttccggcgcg agctggcccc cgcccgcctc gccgacggca cgtcctacat
     4441 ggcatgggtc tattggtacg gcggcgatgt cgctcactgc acgcagatcc ccggcggcga
     4501 ttacctcgcg ctgcttgccc gcgaagaact tgcccccgcc ggcatcgatc cgggcgtcct
     4561 gatcaacctc gacggcgcga tctgaaccgc cggttccacg tgaatcgtta gtcgaccgac
     4621 agccgcgcca gcgccgccgc gatttcgctc tgcgccgccg cggccttggt gatcgcctcg
     4681 gcgatcttca aattcgccgc cgccacttcc cgcgtcgccg ccgcctgatc cgccagcggc
     4741 tccaccgagt tcagcttatc gctctgctgc tcgttgcggc tcagatcaag ctgcgcgtgc
     4801 gcggcgggca ggctcacata cggctgcgcc accagcacca gcagcagcat cgccagcacc
     4861 gtcagcacca cgttggtata ggtccgcgtt tcgatgctct tcatctttag gctccttctt
     4921 ccttcgccgg ccgccggttg acggatgcct gttcgccctg tcatcatcgc ggtgccggcc
     4981 gcgggttggg actcgtcttc cacttaacgt cctttatcgg caccccggcc ctccagatca
     5041 acctctgttc gcgccgcgca cctcgcgccc gccgatccca cgcgtcacac gtcgcctcgc
     5101 ggtcgcctcg cccatgaccc ctgttccctc ccccgacccg cgcgccgcgg cagcctattg
     5161 gaaccagatc ctcgacccgc gcaatctggc gcacgcgccc gccgccgaga cgccgcgcgc
     5221 catccagcgc gacatcgccc tggcccgcac gcccgatttc ctggccgctc tgcgccatct
     5281 ggctccgacc gcaagccgtc ccccgggcgt catcgatctg ggcgcgggcc tcggcaacca
     5341 tgcgttggcc gccgcccgga tgggccaccg cgtcctggcg ctcgacgcct ccctggatcg
     5401 gatgcgccgg ctgcggatcc gagcggcgcg cgtgggcgtg cagcttccag ccgtcgtcgc
     5461 ccgcgccgag gcgctcccgt tccgcaccgg cagcgtcggg gcgctctata cgaagtccgt
     5521 cctgatccac accgatctgg accgcgcatt ggacgaaatc gcccgcatcc tgggcccggg
     5581 cgggcgctgc gcgctcatcg aaccccagcc gggcaatccc tttgttcgcc tctaccgggc
     5641 gacgctggcc ccgtccgcct ggaagtccat tacgcgctac ttcgatgccc cgcggcagcg
     5701 gctggtcgcc cagcggatcg gccccacgcg cctccgaccc ttctacttcc ttggctttct
     5761 tgggtttatc ttcgagttcg gcgtcccctg gccgaaggtc cgcaacggcg tgctcgcgtg
     5821 cctgcggccc gtggatgcgc tgcttttcag tctgatcccc ctgctgcgac ggtgggcctg
     5881 gttcgggctg ctcgaagcgg aaaaacctgc cgccaagctc aacgctgagg ccgattctga
     5941 cggcgatttg tcgtcaacag attgacatgt caattcgatt ctgcttccga atcgccattt
     6001 tcatgacagc catggagttc ctttcccacc gaccgggcag ttcatgccgc cgcatgcgca
     6061 tgtggaccct cggcacccgc tcgctccgat tcaaatgatt gaaacaagac gacttgaggg
     6121 atttcatagg ctcctgtgaa cggcggtgac gatctcgggc accgcgcctc ccgccacccg
     6181 gcaatcgctt attacccctt gtggcatggc agttgcccca tgcccgtggc cgatgaactc
     6241 tcggtccggg aaggacttcg ttcgcggcat gcgtttccat cgccctctcg ccctgctcct
     6301 cttcgcgctc ccctgctggg cggcggccca gccgaacccc tccgcttcgg cccccacgtc
     6361 ggccccgcag ttgcttgacg tccggctgcg cccgctcggc gattcgttgc gcatcatttt
     6421 cccgcttacg aatcaggccg cctacagcgt caaacccgag ctggcgtcgc gccgcgtccg
     6481 cattgatttc atcggcgctg aaatccgctt tctccccccc gaactcaacc gcgtcggcct
     6541 cccctggctc gacggaatct ggctggcttc gtcgccccca ggcatcgcca cgctcgaatt
     6601 gcgtctcccc tcgccgcgga tgcggatcac gcactttgcc ctgaccgacc cgccggcggt
     6661 cgttatcgat ctatttcgcg aagatcagcc ctgggtccat acgacggtgg cctccgacgg
     6721 tccgctgccg gccgtccggc tccccgcgtc cgacgaggat cgttccagtt ccgcctccgc
     6781 gtcgctgggc aacagccccg tcgcccagga accggtgaat gaaattcgcc tgatcgacgc
     6841 cgattacgat tacttcccca tcagccagca tgaattccgc tcggccgaag ccaacgaagt
     6901 cctcgacgat tttctcgccc ggcgctgggc ccatgtcgtc cgcaagggga ttgagtatct
     6961 gaaaaccaaa ccgatcaacc cggacgaaac cggcttcctg cttcacctgg tcgccgaggc
     7021 ccgctggcgg ctgggccgcg acacgtttcc ccagccgctc gatgaaatgc ggaattacta
     7081 tgagcaggcc ctgcgcgtcg ccggcgcggg cgacctgggc gccttcgccc actggcgcct
     7141 ggcgcagttg gccggccaga tgcacgattt cgaccgcgcc ctgcagcacc tgcggcaggc
     7201 ggcggcaacc tcccagcccg cgcagcaact gcgcgttgag ctgctgcgtg tgcaggccct
     7261 gcgcgccgcg cggaaaacca acccggcgct caagcagatc caggccttgc tcgcgggcgc
     7321 ggcgctcccc aacggcgtcg aactctccaa cgaacagcgc gtggaactcc tcctcgatcg
     7381 cggcgacatc ctgaccgaaa cgggtcaatt cgaagccgcc tggcaggcct accaggaagc
     7441 gcgaaagatc gatcccgcat ggctcagctt gaatcccgac agcggggagt tgatggcgct
     7501 ggccgcggcc gagatcggcg aactgaccac ggcccgccag accctcgaat acatcaacca
     7561 gcacttcagc tatcgcgaag atgaacgcaa ggtggcgctg gccctccagt tcgccgacgt
     7621 cctggaacgg ctgggcgacc tggacgccgc cgagggcacc tatgccgcgc tgctggccga
     7681 attgggcgac acccccaagg gggccgccgt cgagcggcgc atgctcaagg tttttccgga
     7741 ccgcgtcgtc ggcggcgaaa cccgctactg ccggctcctc tggcgccggg gcaaactgac
     7801 gcaggcgatg actgaactcg accgcgtctg gaatctgtgt ctgcgcgagg ggatcagcac
     7861 cgagccgctg cgcgagctcg tcggcaagat cgtcccggac ttcatcgcgt tctgcgcgcg
     7921 ccacgaccag ccgttcgccg cggtgcaagc ctggaatctc tatcaggcca gcatcggcaa
     7981 ttctgccgcg cgccagtcgt gcatgccgcc gctcatcgaa tccttggccc tgctgaacct
     8041 gaatgacgaa gcgctgcggc aactgctgct ctacgaacaa catgcgacgg ccgacgcgga
     8101 ggactccgcg caccttgctt tgctcaaggc gcaactgctg agccggctgg agcgcaccgt
     8161 cgaagccatc gaactgatcg aaaagaacct gacgcggatc gacgatcccg atcagtcggc
     8221 caaagccctg cggctgctgg ccgaagccta cagcgccgag gatcgcccgc tggaagccgc
     8281 gcaagcctgg caggccctgg ccgaagtcaa cccggccgat tccaccaccg tcggcgaagc
     8341 gagcctgcgc gccggcgaaa tcttcctgga gcaggcgatg cccctgcagg cgatggaaat
     8401 cggcctgaag gccctgttcc atgagcagca acacctggac cagggactcc ccgtgacatg
     8461 ggcgcccgag gtccgcgacg gcttgctcct gatgctcgcc aaagcctacc gcgcgcgcgg
     8521 cgaccagcag cgcgcgctca tcctgctcga tgagtatctc gcgcggccct ccatcaccgc
     8581 cggcgaggcg gcgttcgcgc gccggatggt cgccgagtgt caccgcgagg cgggccgact
     8641 ggacgcaagc cgcgccgcct acgaggcgat cctcgccgac gaacgggccc ccgcgccctg
     8701 gccgcagatc gcccgtcgcg ccctcgacga gctctcctgg aacgagcaga acccccagtg
     8761 gtcgatcccg cgcccgcaaa ggaattgacg atggccgccc gcttgacgca aaccacgccc
     8821 gcgcccccgg cgccgcacga cccctcgacc tccgcgccga catccctccg gatgcgccag
     8881 gcgatgcaga tgctgcgcgg cgccgaacac aagatcgccg atgcttatca gcagctcggg
     8941 gcggaagtgc agcgcctgac cgccgaactc gattccagca acctggaatt gcgacgcaac
     9001 ctggccgaac gcgaaaagat gcaggcgatt ctgatctcga cgctgcagag cttgacgatc
     9061 ggcgtgctgg ccgtcggtca ggacggcgtc gtgatcgtcg ccaatcccgc ggcctgcgag
     9121 atcttccgcc gcccattgga gcaaatcacc acgcgccatc tggaagagat cctcgacggc
     9181 atccccgaca gcggtcagct tctgctgacg ctgcaagggg ccggcggcgg ccagagccgg
     9241 atcgcctgga gccaacccgt cgatcagggc gcgccgcgtc agatcgaact gaccgcggtc
     9301 cggtcgctgc cgccttcgga tatccatctg gccggtctga tcctcgccga ggatcagacc
     9361 cacctgcgcc ggctcgagca ccaggccacg ctgcgttcgc gcctgaccgg catgggtgaa
     9421 atcgcaatga atctggccca cgaaatccgc aacccgctgg ggtcgattgc cctgttcgcc
     9481 acggcgctgg agcgcgaatt ggaagggaat gagtcgctcg ccccgatggc gcgccagatc
     9541 gtcgccggcg tcaaatcgct cgagcatctg gtcgccaaca cgctggagtt tgcccgtccg
     9601 cgccgcatgg cgatggcgcg cgtcaacctg aaggatgtca ttcgcgacac gctggtctac
     9661 cttgagcatc cgctcgagca gcatgcgatc caggtccagt acgatcacga gctccagccc
     9721 gacgcctggg tttcggccga cgccgagcag cttcgccagg tcttcctgaa tctgggactc
     9781 aacgcgattc aggcgatgga cgacggcggc aagctcgaat tcctgattca ggccgatgcg
     9841 gccggcggct gggaggtcgc cgtgcgcgac aatggcgtcg gcatcccgcg cgaactgatc
     9901 gagcgcattt tcgatccgtt cttcaccacg cgcgacaagg gctccggcat cggcctggca
     9961 atcgtccact cgatcctgca ggcccacgac gcccgcatcg aggtcgagtc gcgcgtcgcg
    10021 gtcggaacga cgtttcgcgt cgttttcccc gcataccgtc agtttgaagg catttgacgg
    10081 aggaaacccc cgtgatgcga accggaacag atccgaacgg cgatgtcgcc gccccgcgcg
    10141 gcgtcatcct ggtcgccgac gacgaacccg gcatgcgcag cgcgctgcgc gaagtcctgc
    10201 gccgatccgg ctggcgcgtc gaggcggtcg aatgcggcgc gcaggcgctg gcccgcctcg
    10261 ccgaggatga cgccgtcgac ctgctgctga ccgacttccg catgcccggc atgagcggcc
    10321 tggaactgct gcgcgaagcc cgcgcgcagc gtcccgcgct gcccgtcatc atgatgaccg
    10381 ccttcgggac cgtggaggat gccgtcgccg cgatgcgcga cggggccgcc gattatctga
    10441 tgaaaccctt ttcgttcgac accgtgacgc agaccgtcga acgcgtgatg gccgaacgcc
    10501 tcgacccgcc gcggacgccg accggccgac cggcggagag cgccaccccg gtgctgcccg
    10561 cggcccgcgg cggcaacatc catgcgcgca tcatcgccga aagccccagc ctgcgtgcgc
    10621 tcctcgagat tgccgccgac gtggccgaag ccgacagcac ggtgatgctg agcggcgaaa
    10681 gcggcaccgg caaggaagtc gtcgcccgct tcatccatga atgcagcaac cgcaaggggc
    10741 cgttcgtcgc catcaattgc gcggcgctcc ccgaaggctt gctcgaaagc gaattgttcg
    10801 gccacgaaaa gggggccttc accggcgccg tcctggcgcg gaaggggcgt ttcgaacagg
    10861 ccaacggcgg caccctgctg ctcgatgaaa tcagtgaaat gcccctggcg ctgcaggcca
    10921 aactgctgcg cgttctgcag gaacgggaga ttacccccat cggcgccaat gaaacgattc
    10981 ggctcgacgt gcgggtgatc gcgaccacca accgcgatct gcgccaggag gtcatggaac
    11041 atcgcttccg ccaggacctc tattatcgtt tgaacgtcat tgcattggat attccgccgc
    11101 tgcgcaatcg ccgcgaggat atcctgccgt tggccgagta tttcctcgcc cgctatcggc
    11161 gtaaaaaccg cccggctcaa cggcttggcg cggatgtccg ccgctacctg ctggattgcg
    11221 aatggccggg caatgtccgg gaattggaaa acctgatcga gcgggcgtgc ctgctcgcgc
    11281 gcgggaccga gatcacgctc gcggatcttc acctcgggcc ggcgcgtcag tcaacgccgg
    11341 ccggcgccat gcccttcccg ttcggcgacg actgtctcac gctcgaggaa ctggaacggc
    11401 ggctgatcac gcacacgctg gaacgcaccg gcggcaaccg cacccgcacc gccgacctgc
    11461 tcggcgtcag cgtgcgcacc attcgcaata agattcacca gtatggcctg gtcgaagccg
    11521 ccggctgagc agccggcgac cgattccatt gttccgcttc cacgtgccga cggtaatccg
    11581 caatggaaac gccgcggtcc ttcagggcgc cgcgcaaggc ctgaacgcgc ggacttagtt
    11641 cctcatccgc agcctgacac cgcgagccaa gggcggcaaa ataatccgcc accagacccg
    11701 acagggatcg gccctgctcg cggcaaatgc gcttggccta acggacgaga tcttcatcga
    11761 ttcgcagcgt aagtttcgtt tgcatgagct gctctcccgg atgtgcatct ccggatacgt
    11821 caccgtcgcg cgtgtaatcc acccgatgca ccagagggtg cggtcgcgcc ggcgcaccct
    11881 cacaaccgct tcaggaagcg ctccaatccc gccagcgccc gctcgatcgt ctggaaactg
    11941 gccgcatacg ccatccgcac gtaaccctcg cccgcctcgc cgaacgcatt gcccggcacg
    12001 atcgcgaccg attcctcctc cagcagtttg cgacagaagg tctccgagtc caaccccgta
    12061 tgccggatcg acggaaaggc gtagaacgcg ccggccggcc ggtgacaggg caggccgatc
    12121 tcgttgagcc ccttgacgat catgttgcgc cgctggcggt aaatcgcgca catttcgcgc
    12181 atgtcgtcca gcccttcggt cagcgccgcc tcccccgccc gctgcgccat gattggcgcg
    12241 cacatgatcg agtactgatg gattttcgtc attgcggcga tcacctcgcg cggcccggcg
    12301 gcgtagccca gccgccaccc ggtcatcgcg aacgccttgc tgaaccccga cagcagcacc
    12361 gtccactccg ccgcgcccgg gatcgccgcc aggctcacat ggtcgccgtc gtgcgtcagc
    12421 tccgcgtaga cctcgtcgct gatcatcaga atctggcgcg gcacgcagat ccgcacgagc
    12481 ccttccagat cctcgcggcg cagcgtcgcc cccgtcggat tggacggata attaatgaaa
    12541 acggcccgcg tccggtccgt gatcaggttt tccagcgcgt ttaaatcgag accgaagccc
    12601 gcctcgatcg aggtgttgag cgccaccggc acgccccccg ccagtgtcac cagcggcgca
    12661 tacgccacgt agctcggctg ccagatgatc acctcgtcgc ccggctccag gatcgcgcgc
    12721 aggaccagat ccagcccctc gctcaccccc accgtcagca gcatctcgtc ttccggatca
    12781 aaggtcgcct tgaaccgttc gtgcaggtag cgcgcaatcg ccgcgcgcgt gctcaacagc
    12841 ccggaattcg acgtgtaggt cgtgtcgccc tgactgatcc ggtagagcgc cgtctcgcgg
    12901 atgtgccacg gcgtgacgaa atcgggttcg ccgacgccga gcgaaatcac atcatcgcgg
    12961 ccgatgacca gctcgaaaaa cgcccggatc cctgacggtg gcagcgttcg caccttctcc
    13021 gccacccgcg gccgagtctc ccgccgcgtc gcaaccttcc gcacttctgc catggtctgc
    13081 ttccttccct tgtcgaatat ccgtctctcg cccgcaatct aaatcgaccc tcgtctctcg
    13141 tcctcgtat
//
LOCUS       DAOPCO010000086        12985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4783_length_12985_cov_5.376102, whole genome shotgun sequence.
ACCESSION   DAOPCO010000086 DAOPCO010000000
VERSION     DAOPCO010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12985
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4783_length_12985_cov_5.376102"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLS90_07830"
     CDS             complement(<1..598)
                     /locus_tag="PLS90_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02353.1"
                     /translation="MNSALTRSARAITLVELLIALTIAMAFLSGVYATFIHVVRAHDT
                     AEAREQAAANGRVALSTLSEDFKEVNTMGPDVFFMGIDGGLDYGDGIDNDGDGLIDEE
                     IVDGLNESPTPALAADRHARIGDRFERPQYVDREDLGDRDVDVGRSGLRQPGQLRPGH
                     RRVDGEGLGSRGGDHPPGEPGDQLGGHGIRGRGRSAERR"
     gene            complement(579..1001)
                     /locus_tag="PLS90_07835"
     CDS             complement(579..1001)
                     /locus_tag="PLS90_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK02354.1"
                     /translation="MRRQRAHAFSLLEVLVGLSILLAGLLAIAAFFPTMIAGQRDAEL
                     LTSAAALAQLKAEEIRRDNDSAGQLIAAIRDLDEPTEPLAFPSDPRLAYSFCGETLLY
                     QDAPAGDPRAAADVARIIVRKAADFDPDQEVIYELRFD"
     gene            complement(998..1570)
                     /locus_tag="PLS90_07840"
     CDS             complement(998..1570)
                     /locus_tag="PLS90_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02355.1"
                     /translation="MTRLLTIARSPRRASGRAGFSMVELIAVIVIGLILASVAMAGVR
                     IYERDLPVRQQASRLVKAFASARSFAIANNSFFAVQYSPELGIFWIDETDAGGGPLVH
                     KVMRPESIDDKVAVDAIFFGANAADLNQDRLSILFRPDGSSDDVRIFLRMLGAAATDA
                     TQTHTVRLYGPTGQARVFENQRLEPTAATP"
     gene            complement(2440..3411)
                     /locus_tag="PLS90_07845"
     CDS             complement(2440..3411)
                     /locus_tag="PLS90_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02356.1"
                     /translation="MFSLRPSATRCSPPAPLVGLAGGLLRAAASVLLVAWGVATVTFM
                     LMRAIPGGPLSRERTPPAAVRNAIAARYHLDDPLWRQYVRYLGDAARGTFGLSYDHPG
                     RTVGEIIAQRLPVSAVLGALALGLALAAAFPLGVAGALHQGRWPDRLNAGFAAAGVSV
                     PSFILGALLLYTFAYRLRWFPATGWFGLSSAILPAAALAALPAAYLARLIRSELVEVL
                     HSEFLLTARAKGLSLPRVVLVHALRHTLSPVLAYLGPQAAAIMTGSFVIERIFNIPGL
                     GEQFVTSITNRNYPMIMGVTLLYTVMLVVFNLAADALARWLDPRLRQ"
     gene            complement(3421..5265)
                     /locus_tag="PLS90_07850"
     CDS             complement(3421..5265)
                     /locus_tag="PLS90_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02357.1"
                     /translation="MLDALVLCFSFHWPACATAAAHAALVDRALAPLLACAARRPRLP
                     LTLHLTGPLLEHLAQSSPDLLEELAGLVARSQVEPLGGPWAPCLLPLLDPASQDDQLA
                     ALADACHARLDARPAGALPTGWVWDHETAARLARHGYAYALLPEARLRAARPEPPAAG
                     LPAVTTQGGPLRLLPVAAVLPGDADAAQIEQGLRALAAARAAGFGAAALTWLFTPERP
                     PRDLNAALAPVIRLTDAAREAPFALAGTAVARAAAGDEIHLPDTRYSELCRHWPGADS
                     QPAAPWMDLASWRAGLLRDPANRAWQAGCRAVSAQLAAAAPGAPELGRARRLLRVAQS
                     AEALQVGQPPGRATAATRHALAAARNALARIHHGDAPWVASSQEADPAVLLESDRWVL
                     AADPAGGGLRHLHYRPRDLSLLGDRGFPGDVVFGADRFLPRDASAADPESVRAAAAGA
                     GPLQHRLLASPRALRLGLHREFELELEGRSGALSLRKSVILERGEDEFTLECVLKNTA
                     AAVLPLAWAIELNLRAGAIGALWVHAPDAPAPAAGAVLTHLLQFGFDDPRRNLRIRFS
                     FSWPCELTLPPAEPAAEGLLTLFPIWRLELEPGIPRPIAVTVDARPLF"
     gene            complement(5485..5751)
                     /locus_tag="PLS90_07855"
     CDS             complement(5485..5751)
                     /locus_tag="PLS90_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HPK02358.1"
                     /translation="MVSWRLVYTAQARRDAKKLARGDLKPEAQRLLELLADDPLKSPP
                     PYENLRGDLAGALTRRISLQHRLVYQVFQETRTFKVIRMWTHYE"
     gene            complement(5745..5987)
                     /locus_tag="PLS90_07860"
     CDS             complement(5745..5987)
                     /locus_tag="PLS90_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HPK02359.1"
                     /translation="MSVISATEARKQLYSLLDEVAASHELVRIAGRRNSAVLISEQDW
                     PSIQETLHLLSIPGMRESIRKGLATPVDKCSEKRDW"
     gene            6294..6596
                     /locus_tag="PLS90_07865"
     CDS             6294..6596
                     /locus_tag="PLS90_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK02360.1"
                     /translation="MAATSTKKAMTKTQLVGTLAEKSGISKKEAGELLNVLAETAYKE
                     AKRGFTIPGIGKLVVVKRKARKGRNPATGETIKIPAKTVVKFRIAKACKDAVTPAK"
     gene            complement(6730..8202)
                     /locus_tag="PLS90_07870"
     CDS             complement(6730..8202)
                     /locus_tag="PLS90_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPK02361.1"
                     /translation="MSGRNWLTTLTLTGLILGAIVGEFYYRRAADVDNGPARLAEHDA
                     LVARKNADDAAWTPADQVRLDALRGEIGPVLSKRAFGRAASYIGSDIFMGLLKMLIMP
                     LIVSSVIVGVTSAGDFSRLGRMAGVTFCYFFSTMLLAVALGLVLVSVIQPGRGFFDGP
                     GGDEARATYLTMGAERRGEMEAMHATEGVDSPKTTMDALMGILIRMVPNNPVDDLARG
                     QTLPVIVFSIFFGIILSMIGPSGRPLIDLMRALMDVMIKMTEYVLCLAPVGVFFLVGR
                     AVAGIGLETFAQKIGVYMFTVIAGLLLHGFVVLPLILWIFGRTNPWRFTIAMREALLL
                     AFSTASSSATLPVTIDCAQTRGGCSRRASGFVLPLGSTVNMDGTALYEAVAVVFLAQA
                     FSGGAIPLSDLIILALTATLAAVGAAGIPEAGLTTMIIVITAVNAGGTVYIPPAAIGL
                     ILGVDRILDMTRTMINVWGDAVGARIITRREPDGGAPEAG"
     gene            8570..10036
                     /locus_tag="PLS90_07875"
     CDS             8570..10036
                     /locus_tag="PLS90_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02362.1"
                     /translation="MPAPEDPRHREIDALFGDPNDALAADPRGAEQPPADAPRRDQSD
                     PLDDLLEAIDNALGGDDYRQFDTSHAMRRNVPADDVLRRADQLFGNPEDTAANQGIAR
                     GDIYRPAEKPQPAPAAEAHETSAKIRLPEETGAPGLTGTPDEPPRTQQGFTVYPSFGP
                     HAVEPEEDEPPPMRPPAPPPVDDGPIDFPSLDEEPAPPQASPPERARAAADAAVPAAE
                     LPTQRQFDDTIDSGHLNARKAETYRGTTQCDLEEDGRYALLIAPRSRVDMAVLAAVMG
                     PVLGLSPEGTRHAILRRRGILIENQPLSVAAGIANQLSHCGQPVLLVLQHRCEESGEP
                     ADLMMLEEHDGGLRLATAAEVFHCRWGQVICLGAGQVRLAPGAPPRRVLDLLLHEPAR
                     HFRAWEATVRAKAPDLGAQNPTLREIAGWLVARCPQAIHTRSLESWIAGSEPDGLPAF
                     ASLIEYHNFMRWHVLAHYAPAKPFRARRRPPDPSPQIT"
     gene            10089..10703
                     /locus_tag="PLS90_07880"
     CDS             10089..10703
                     /locus_tag="PLS90_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02363.1"
                     /translation="MEIDRALNQIEQIHDHLARGEVVRDWRWGPVSLTGVLALAAGAL
                     QSALIGPEPTGPAFLVYWLGLAALAALVGGAAVVKSYLAQPSAWARRQTRIVLAQFAP
                     TVAVATLLTVSFAGAGAAGIGRLPALWALVMGLGTFAARPYLPRAIGWAGLYYLLAGG
                     LLLAFADRSFAFLGWGMALAFGPPQILAGLVFHWNLERHGGPRA"
     gene            10700..11092
                     /locus_tag="PLS90_07885"
     CDS             10700..11092
                     /locus_tag="PLS90_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HPK02364.1"
                     /translation="MTPRAYEPGRFAYEGLDRVLHEKARLGILTSLATRPEGLRFNEL
                     KELCSLSDGNLSRHLQTLEEAGLVEVWKGWRDRRPRTLVRLSADGRRRFVEYLEELER
                     VVRDAAAARRRSAEAGGRAEGEDEWAPA"
     gene            11153..12721
                     /locus_tag="PLS90_07890"
     CDS             11153..12721
                     /locus_tag="PLS90_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HPK02365.1"
                     /translation="MTDPAAAQAQPDLDLLARADFAADAAGRAVARTFSLALLLGVAA
                     DLFFHGRPAGLSVMLYVILYWSALAALLRERYARAPAFAHGLFLPIALLGLAFALFAN
                     PTLSFLNGWLLVGLLGLQSILLAGRSRWAWHQLPILADLLALPPLSIHDGAVALRVGL
                     NGLPLNEDRRQTAVKVLAGIVLALPVLLVGFLLLRSADAVFASGAAWVGEQLAGLELQ
                     PAARHCLFILIVSGGALGLLWALTREPVAAAARTLAPPRFIDPVVIATMLALLNLLYL
                     LFCAVQFAYLFSTGERALPAGFTYAEYARRGFYELCLVTGLNIACVLPGLFMVCPGGR
                     RSSLLLRSLQTLLVAQSGVILASAFMRLSLYEAAYGYTATRLFARTMILLMAATLAML
                     AARVWLERFPFAKAFLLTALALYLGLNYFNGEAFIARRNIDRHLAGADLDLPYLQRLS
                     HDAVPELMRLLDSPDPLIRQGVRDHLAQRRRALADGEAWVSWNRARQRARALLQNDVP
                     HADPIQLQPTGDPS"
     gene            12718..>12985
                     /locus_tag="PLS90_07895"
     CDS             12718..>12985
                     /locus_tag="PLS90_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPK02366.1"
                     /translation="MKIIRAEAMGMCFGVRDALGVTRRIADPASVTIHGELVHNPRVV
                     RELSARGFTVLDETERRDATPATSRVMITAHGISERERKRLEERG"
BASE COUNT         2072 a         4358 c         4638 g         1917 t
ORIGIN      
        1 gccgccgctc ggcggatcgt ccacggcccc gtatgccgtg ccccccgagc tggtcgcccg
       61 gctcgcccgg cgggtggtcg ccgccccggg agccgagacc ttcgccgtcc acacgccgat
      121 gaccggggcg gagctggccc ggctgccgca gtccactgcg gccgacgtcg acgtcgcggt
      181 cgcccagatc ctcgcggtcg acgtattgcg ggcgctcgaa gcggtcgccg atgcgcgcgt
      241 ggcggtcggc ggcgagcgcg ggggtcggcg attcgttgag gccgtccacg atttcctcgt
      301 cgatcaggcc gtcgccgtcg ttgtcgatcc catcgccgta gtcgagcccg ccatcaatcc
      361 ccatgaaaaa cacatcgggg cccatcgtgt tgacttcctt gaaatcctcg ctgagcgtgc
      421 tcagcgcgac gcgaccgttg gcggcggcct gttcgcgcgc ttcggcggta tcatgcgcgc
      481 ggacgacatg gatgaacgtg gcgtagacgc ccgagaggaa cgccatcgcg atggtcaggg
      541 cgatcagcag ctcgaccagc gtgatcgcgc gggcggacct agtcaaagcg gagttcatag
      601 atcacttcct ggtccggatc gaagtccgcc gctttgcgaa cgatgatgcg ggcgacatcc
      661 gcggccgcgc gcggatcgcc cgccggcgcg tcctggtaca gcagggtttc accgcagaaa
      721 ctgtaggcca gccgcggatc cgaggggaag gcgagcggtt cggtcggctc gtccaggtcg
      781 cggatggcgg cgatcaattg acccgccgag tcgttgtcgc ggcgaatctc ctcggccttc
      841 agctgggcca gtgccgccgc cgaagtcagc aactcggcgt cgcgctggcc ggcgatcatc
      901 gtcgggaaga acgccgcgat cgcgagcaat ccggccagca gaatcgacag gccgacgagg
      961 acctcgagca gcgagaaggc gtgggcgcgc tggcgtctca tggggtcgcc gccgtcggct
     1021 ccagccgctg gttttcaaag acgcgcgcct ggccggtcgg gccatagagc cggaccgtat
     1081 gggtctgcgt cgcgtcggtc gcggcggcgc ccagcatccg caggaagatc cggacatcgt
     1141 cgctgctgcc gtcgggccgg aacaggattg agaggcgatc ctggttgagg tcggcggcgt
     1201 tggcgccgaa gaagatggcg tccacggcga ccttgtcgtc gattgactcg gggcgcatga
     1261 ccttgtggac gagcgggccg ccgccggcgt cggtttcgtc gatccagaaa atgcccagct
     1321 cggggctgta ttgcaccgcg aagaacgaat tattggcgat cgcaaacgag cgcgcgctgg
     1381 cgaaggcctt gaccaggcgg gaagcctgct ggcgcaccgg caagtcgcgc tcatagatcc
     1441 gcacgccggc catcgcaacc gaggccagga tcaggccaat gacgatcacg gcgatcagct
     1501 cgaccatgga aaagccggcg cggccggagg cgcggcgcgg ggagcgggcg atggtgagca
     1561 ggcgcgtcat ccgcggaact ccggttaccg ggccgaggca tcgggcgcgg gggcggcggc
     1621 cgattggctc tgcggggggc ggtctggact ccgcccggcg attcaaccgg cccgttgcag
     1681 cgcatccaac gggcgacggc gggcagcgct ccggcgcgcg gcataaagcc agcaacgaga
     1741 gcgcaaaata cctgattgga gcgtatatcg gaatggaggg ggtgtcaaac tgaacatccc
     1801 cggatgcggc ggagcgggcg aatcggcgaa ggggcggcaa aaggagtgaa agaaagatga
     1861 gccgcggcac gattgctcga cgatgccgcg gctccagtcg cataaggccc ctgggtgaga
     1921 gggaagcgct tattccgttt tcgcggcgat tggcggtcgc cgcaagcagg gcccgatgcc
     1981 acggcaggga catcaggcgg gccgaggacg aaggcttcgc gacccgagcc gcgcccgcgt
     2041 cctcgcgcgg cgacaacttc agggcccgga aaacggggcc gggcccccaa ccgcgcgttg
     2101 ccaggatcaa atcgccacct gggagggtga agcctccttt aacggaagat gcgaagagac
     2161 agttgctatc gcgggtccgg tccgatccgc tgaggaggga gatgagcaaa aagcggaccg
     2221 agtgaaaagc cgccgtgatt cgttaaatat gttaacaagt aagccgaaga ttgccaaaac
     2281 cgtgacaggg cgacgggttg cgggcgtcaa aatgccctga tttacagcat catttttgag
     2341 aaccttctca aaatgataag aaattcggga ttgcccggac gcgatttcgc ggtccccgct
     2401 cgaatgtcga ttccggggcg ccggggtgag aaactgaggt tactgccgga ggcgcgggtc
     2461 gagccaccgc gccagcgcgt cggctgccag attgaacaca accagcatca ccgtgtagag
     2521 cagcgtgacg cccatgatca tcgggtagtt gcggttggtg atcgacgtga cgaactgctc
     2581 gcccagcccg gggatgttga agatgcgctc gatcacgaac gagccggtca tgatcgcggc
     2641 cgcctgcggg ccgagatagg ccagcaccgg cgacagcgtg tggcgcaggg cgtgcaccag
     2701 caccacgcgc ggcagcgaga gtcccttggc gcgcgccgtc agcagaaatt cgctgtggag
     2761 cacctcgacc agctcgctgc ggatcagccg ggccaggtag gccgccggca gcgcggccag
     2821 cgcggcggcc ggcaggatgg ccgacgacag accgaaccag ccggtggccg gaaaccagcg
     2881 cagccggtag gcgaatgtgt agagcagcag cgcccccagg atgaaggaag ggaccgacac
     2941 cccggcggcg gcgaagccgg cattgagccg atcgggccag cgcccctgat gcagcgcccc
     3001 ggcgacgccg agcgggaagg ccgcggccag cgccagaccg agcgccagcg cgcccagcac
     3061 ggccgaaacc ggcaggcgct gtgcgatgat ttcgcccacg gtgcggccgg ggtgatcgta
     3121 cgacaggccg aacgtgcccc gggcggcgtc gcccaggtag cgcacgtact gccgccagag
     3181 cgggtcgtcg agatggtagc gggcggcgat ggcgttgcgc acggcggcgg ggggcgtccg
     3241 ttcgcggctg agcggcccgc cggggatcgc gcgcatgagc atgaacgtga ccgtggcgac
     3301 gccccacgcg accagcagga cgctcgccgc cgcgcggagc agtccgccgg ccaggcccac
     3361 gaggggcgcc gggggggagc atcttgtcgc gctggggcgg agggaaaaca tggggttcct
     3421 tcagaacagc ggacgggcgt cgaccgtcac ggcgatcggc cgcgggatgc cgggttccag
     3481 ctccagccgc cagatgggga acagtgtcag caggccttcc gcagccggct cggcgggcgg
     3541 cagcgtgagc tcgcacggcc aggagaagct gaaccggatc cgcagattgc gccgggggtc
     3601 gtcaaagccg aattgaagaa gatgggtcag cacggcgccg gcggcgggcg ccggcgcgtc
     3661 cggagcatgc acccagaggg cgccgatggc gccggcacgg aggttcaact cgatcgccca
     3721 ggcgagcggc agcaccgccg ccgcggtgtt cttgaggacg cactcgagcg tgaattcatc
     3781 ctcgccgcgt tccaggatga ccgacttgcg caggctcagc gcaccggagc ggccctccag
     3841 ctcgagttcg aattcgcggt gcagaccgag ccgcaaggcg cggggcgagg cgagcaggcg
     3901 gtgttgcaat ggcccggcgc cggcagccgc cgcgcgcacg gactcgggat cggccgccga
     3961 cgcgtcgcgc ggcaggaacc tgtccgcgcc gaagacgacg tcgccgggga accccctgtc
     4021 acccagcaga ctgagatcgc gcgggcggta gtgcaggtga cgcagcccgc cgcccgcggg
     4081 gtcggccgcg agcacccagc ggtcactttc caggagaacc gccggatcgg cttcttggga
     4141 agaagcgacc cacggggcgt cgccgtggtg gatgcgggcg agggcgttgc gggcggcggc
     4201 gagtgcgtgg cgcgtcgcgg ccgtcgcgcg gcccgggggc tgcccgacct ggagcgcctc
     4261 ggcggactgg gcgacgcgca gcaaccggcg cgcgcgcccc agctccggcg caccgggcgc
     4321 cgccgcggcc agttgcgccg acaccgcgcg gcaaccggcc tgccacgcgc ggttggcggg
     4381 gtcgcgcagc agtccggcgc gccacgaggc caggtccatc cacggcgcgg cgggctgaga
     4441 gtcggccccc ggccagtggc ggcacaactc gctgtagcgc gtgtcgggca ggtgaatttc
     4501 gtcgccggcg gcggcgcggg cgaccgccgt gccggccagg gcaaacggcg cttcgcgcgc
     4561 ggcatccgtg agacgaatca ccggcgcgag cgcggcgttc agatcgcgcg gcgggcgttc
     4621 gggcgtgaag agccaggtga gggcggcggc gccgaatccc gccgcgcgcg cggccgccag
     4681 cgcgcgcagc ccctgctcga tctgcgccgc gtcggcatcg ccgggcagca cggccgccac
     4741 cggcagcagg cgcaacggcc cgccttgggt ggtcacggcg ggcaggcccg ccgccggtgg
     4801 ttcgggccgc gccgcccgca gccgcgcttc gggcagcagc gcataggcgt agccgtggcg
     4861 cgccaggcgg gccgcggtct cgtgatccca tacccagccg gtcggcagcg cgccggcggg
     4921 ccgggcgtcc aggcgcgcat ggcaggcgtc ggccagggcg gcgagctggt cgtcctggct
     4981 ggcgggatca agcagcggga gcaggcacgg cgcccacgga ccgccgagcg gctcgacctg
     5041 gctgcgcgcc acgagcccgg ccaactcctc gagcaggtcg gggctgctct gcgccaggtg
     5101 ctccagcagg gggccggtca ggtggagcgt caggggcagc cggggccggc gggcggcgca
     5161 ggccagcagc ggggcgagcg cgcggtcgac cagcgccgcg tgcgccgccg cagtggcgca
     5221 cgccggccag tggaaactga agcagagaac cagggcatcc aacatactgg atcggttcgc
     5281 ttcggcggga accggcgcgc gcaggcagat cggggcggaa gcgccatccg cccgccctgt
     5341 ttaccccaac cggcggggac ttggaagcta aagggcggga agttgcggca agcgccgagt
     5401 tgaatctggt cgcgggcgtc ggctaactct ctcgctccat tacatcgtca agatgatatg
     5461 caatgactac taagattgtt attcttattc ataatgagtc cacatgcgaa tgactttgaa
     5521 ggttctggtt tcctggaata cctggtagac gagtcggtgt tggaggctga tgcgtcgtgt
     5581 gagagcgccg gcgagatcgc cgcgcaggtt ctcataggga ggcggggact tcagcgggtc
     5641 gtcggccagc agctccagca gtcgttgcgc ctcgggtttc aggtcgccgc gggcgagctt
     5701 tttcgcgtcg cgccgcgcct gggcggtgta aaccagccgc caactcacca gtcacgtttc
     5761 tcggagcact tatcgacggg cgtggccaga cccttccgta tcgactcgcg catgcccggg
     5821 atggagagca aatgcagcgt ttcctggatc gagggccaat cctgttccga gatgagaacc
     5881 gcggaattgc gccggccggc gatgcggacc agttcatggc tcgccgcaac ttcatccagc
     5941 aggctgtaga gttgtttgcg agcttcggtg gctgagatca cggatatggg acaggccctc
     6001 cgggggcgta gtgttaagcg tacgctgtcg gcccgcgtta atctgaagac acataccggc
     6061 aaacatccgg ccgcgtcgcg ggatgcgggc ggacgatggc tcgatctcaa tccggttacc
     6121 gataccgatc agctgccgaa tgtcggtccg gccgctccga cccctgaaaa acaaggcgat
     6181 tcggcccttt tttttgcacg gccggggttg actattgcgg cggacggcac ataatgaccg
     6241 ctagtcatgg tggtggttgc ctgtgactga cttcaatcga gaggagaagg cctatggccg
     6301 caacatctac caagaaggcg atgacgaaga cccaactggt ggggacgctc gccgagaagt
     6361 ccggcatttc caagaaggaa gcgggcgaat tgctcaacgt gctggccgag acggcctaca
     6421 aagaagccaa gcgcgggttc acgattcccg gcatcgggaa actggtcgtc gtgaaacgca
     6481 aggcgcgcaa gggtcgcaac ccggcgacgg gcgaaacgat caagatcccg gcgaagacgg
     6541 tcgtcaagtt ccgcatcgcc aaggcgtgca aggacgccgt cacgcccgcc aagtgatcgc
     6601 ggccggcggc cgtgcggtcg ccgcgccccg tcgacgccca cgcagaaaac ctacgccctg
     6661 cccgcggccc ccggcccacg gcagggcgtc ctttttgcct gcgcccgcgg cgcgtccgcg
     6721 gccgccgtgc tagccggcct ccggcgcgcc gccgtcgggc tcgcggcgcg tgatgatccg
     6781 cgcgccgacc gcgtcgcccc acacattgat catcgtgcgc gtcatgtcca gaatccggtc
     6841 cacgcccagg atcaggccga tcgccgcggg cggaatataa accgtcccgc ccgcgttgac
     6901 cgccgtgatg acgatgatca tcgtggtcag gcccgcctcc gggatccccg ccgcgccgac
     6961 ggccgccagc gtcgccgtca gcgccagaat gatcaggtcg ctcaacggga tggccccgcc
     7021 gctgaaggcc tgcgccagga agacgacggc caccgcctcg tagagcgcgg tgccgtccat
     7081 gttgaccgtc gagccgagcg gcaggacaaa gcccgacgcc cgccgactgc agccgccgcg
     7141 ggtctgcgcg cagtcgatcg tcaccggcag cgtggccgag ctggaggcgg tcgagaacgc
     7201 cagcagcagc gcctcgcgca tcgcgatcgt gaagcgccac ggattggtcc ggccgaagat
     7261 ccacaggatc agcggcagca cgacgaaacc gtgcaacagc agcccggcga tcaccgtgaa
     7321 catatagacg ccgattttct gcgcgaacgt ctccagcccg atgccggcca ccgcgcgccc
     7381 gaccaggaag aagacgccga ccggcgccag acagaggaca tactcggtca tcttgatcat
     7441 cacatccatc agcgcgcgca tcaggtcgat cagcggccgg cccgacggcc cgatcatcga
     7501 caggatgatg ccgaagaaga tcgagaagac gatcaccggc agcgtctggc cgcgcgcgag
     7561 gtcgtccacc gggttgttcg gcaccatccg gatcaggatc cccatcaagg catccatcgt
     7621 ggtcttgggc gagtccaccc cctcggtcgc gtgcatcgct tccatttcgc cgcgccgctc
     7681 ggcgcccatg gtcaggtagg tggcgcgcgc ctcatcgccg cccgggccgt cgaaaaagcc
     7741 gcggccgggc tgaatcaccg acaccagcac gaggccgagc gcgaccgcca gcagcatcgt
     7801 ggagaagaag tagcaaaagg tcacgccggc catgcggccc agacgggaaa aatccccggc
     7861 gctcgtcacc ccgacgatca cgctggagac gatcagcggc atgatcagca tcttgagcag
     7921 gcccatgaaa atgtcggagc cgatgtagga ggcggcccgg ccgaaggcgc gcttgctcag
     7981 gaccggtccg atctcgccgc gcagcgcgtc gagccggacc tgatcggcgg gggtccaggc
     8041 ggcgtcgtcg gcgttcttgc gggcgacgag cgcatcatgt tcagccagcc gcgccggccc
     8101 gttgtcgacg tcggcggccc ggcgatagta gaactctccc acgatcgcgc cgaggatcag
     8161 cccggtcagc gtcaacgtcg tcagccagtt gcgtcccgac atgaagtctc cctctcgttc
     8221 gcgcgggcga tcgcatggat aggccgccag atgcaaaagt ccgtcagtct accgtctggc
     8281 ggggggagag acaagtacca gcgccgggcg ctgaatgtga tcggcccctt tctcatcggg
     8341 atcgggattg gcatcgccat cgtgcttccg ctgttcccgg gcagcgcgcc gcgcatcctg
     8401 cttgacttgg cacggcacgt ggtaaggatg aaggaagggg gtgaacccgg cgcgcgccgg
     8461 ccgcctcgat tccgggccgc tgctccgccg gccgttcagc atcccgtgcg cgtcgtcccg
     8521 cccggccgcg cgccccccgc cggatcccgc ggagcaggag acttcctcca tgccagcgcc
     8581 cgaagatccc cgtcatcgcg agatcgacgc gctgttcggc gaccccaacg acgcgcttgc
     8641 ggccgatccg cgcggcgcgg agcagccgcc ggccgatgca ccccgccgcg atcagtccga
     8701 cccgctggat gatctgctcg aggcgatcga caacgcgctg gggggcgacg actaccggca
     8761 gttcgacacc agccacgcga tgcggcgcaa tgttccggcc gacgacgtgt tgcgccgggc
     8821 cgatcagctt ttcggcaacc cggaggatac cgccgccaat caggggatcg cgcgcggcga
     8881 catttaccgc ccggccgaga agccgcaacc ggcgccggcc gcggaggcgc acgagacgtc
     8941 cgcgaagatc cggctgccgg aagaaaccgg cgcgccggga ctgaccggaa cgcccgacga
     9001 accgccgcga acacaacagg ggttcacggt ctacccttcg tttgggccgc atgccgtcga
     9061 accggaggaa gatgaaccgc cgcccatgcg gccgccggcg ccgccgccgg tcgatgacgg
     9121 cccgatcgac ttcccttcgc tggacgagga gccggcgccg ccccaggcca gcccgcccga
     9181 acgcgcgcgc gccgccgccg atgccgccgt tcccgccgcc gaactgccca cgcagcgtca
     9241 gttcgacgat acgatcgaca gcggccacct caatgcgcgc aaggccgaga cctatcgcgg
     9301 cacgacgcaa tgcgatctgg aggaggatgg ccgctacgcg ctgctgatcg cgccgcgcag
     9361 ccgcgtcgac atggcggtgc tcgccgccgt catgggtccg gtgctcggcc ttagccccga
     9421 agggacgcgc cacgcgattc tgcggcgccg ggggattctg atcgagaacc agccgctgtc
     9481 ggtcgccgcc ggcatcgcca atcagttgag ccactgtggc cagccggtgc tgctggtgct
     9541 ccagcaccgc tgcgaggagt ccggcgagcc ggccgacctg atgatgctcg aggagcacga
     9601 cggcgggttg cggctggcga ccgccgccga ggtatttcac tgccgctggg gccaggtgat
     9661 ctgtctcggg gcggggcagg tgcggctggc gccgggcgcg ccgccgcggc gggtgctcga
     9721 tctgctgctg cacgaaccgg cgcggcattt ccgcgcatgg gaagcgacgg tccgggcgaa
     9781 ggcgccggat ctgggcgcgc agaatccgac gctgcgcgag atcgcgggct ggctggtcgc
     9841 tcgctgcccg caggcgatcc atacgcgctc gctggagtcg tggatcgccg ggtcggaacc
     9901 cgacggtctc ccggccttcg ccagcctgat cgaatatcac aacttcatgc gctggcatgt
     9961 gctggcgcac tacgcgccgg ccaagccgtt ccgggcgcgg cggcggccgc cggacccgtc
    10021 tccgcaaatt acttgacgac gcaaagtgca tacggtaggc tgcgcgcatt caaccccggg
    10081 tgcgggccat ggaaatcgat cgcgcgctca atcagattga acagatccac gatcatctcg
    10141 cgcgcggcga agtcgtccgc gactggcgct ggggaccggt cagtctgacc ggcgtgctgg
    10201 cgctcgccgc cggcgcgctg caatcggccc tgatcggccc cgagccgacc ggcccggcgt
    10261 tcctggtcta ctggctgggg ttggccgcgc tggcggcgct cgtggggggc gccgccgtgg
    10321 tcaagagcta cctggcgcag ccgagcgcgt gggcgcggcg gcagacgcgg atcgtcctgg
    10381 cgcagttcgc cccgacggtc gcggtcgcga cgctgctgac cgtttcgttt gcgggcgccg
    10441 gcgcggcggg catcggccgg ctgccggcgc tgtgggcgct ggtgatgggc ctgggcacgt
    10501 tcgcggcgcg cccgtacctg ccccgcgcga tcggttgggc cggcctgtat tacctgctcg
    10561 cggggggcct gctgctggcg ttcgccgacc ggtcgtttgc gtttctcggc tgggggatgg
    10621 cgctggcgtt cggcccgccg cagatcctag ccgggctggt gttccactgg aatctggagc
    10681 gccacggagg tccgcgagca tgacgccgcg cgcctacgaa ccgggtcgtt tcgcctacga
    10741 ggggctcgat cgcgtgctgc acgagaaggc gcggctgggg attctgacgt cgctggcgac
    10801 ccggccggag gggctgcggt tcaacgaact gaaggaactc tgctcgctga gcgacggcaa
    10861 cctcagccgg cacctgcaga cgctggaaga ggcggggctg gtggaggtgt ggaagggctg
    10921 gcgcgatcgg cggccgcgga cgctggtgcg gctgtcggcc gacgggcggc ggcggtttgt
    10981 ggagtatctg gaggaactgg aacgggtggt gcgcgacgcg gcggccgcgc ggcggcggtc
    11041 ggcggaagcc ggcggccgag ccgaggggga ggacgagtgg gcgccggcgt gaaatttttt
    11101 tcccctgatg ctttgcattg caaagtttac cccgctccca ggagctgacc cgatgaccga
    11161 tcccgccgcc gcccaggccc agcccgacct ggatctgctt gcgcgcgccg acttcgccgc
    11221 cgacgccgcc ggccgcgccg tcgcccggac cttcagcctc gccctgctgc tcggcgtcgc
    11281 cgccgacctg ttctttcacg gacgccccgc cggtctctcc gtcatgcttt acgtcattct
    11341 ttactggagc gcgttggcgg cgctcctgcg cgagcggtac gcgcgggcgc ccgcgttcgc
    11401 gcatggcctg ttcctgccga tcgccctgct ggggctggcc ttcgcgctgt ttgccaaccc
    11461 gaccctgagc tttctcaacg gttggctgct ggtggggctc ctcgggctcc agtcgattct
    11521 gctggcgggc cgcagccgct gggcctggca tcagctcccg atcctggcgg acctgctggc
    11581 gctcccgccg ctgtcgatcc atgacggcgc ggttgctttg cgcgtcgggc tcaacgggtt
    11641 gccgctgaat gaagaccgcc ggcaaaccgc cgtgaaggtc ctcgcgggca tcgtgctcgc
    11701 gctgccggtg ctgctggtgg gcttcctgct gctgcggtcg gccgatgccg tcttcgcgag
    11761 cggggccgcg tgggtcggtg agcaactcgc cgggctggaa ctgcaacccg ccgcccggca
    11821 ctgcctgttc attctgatcg tcagcggggg cgcgctcggg ttgctgtggg cgctgacccg
    11881 cgagccggtc gccgccgcgg cccgaacgct cgcgcccccg cgcttcatcg atcccgtcgt
    11941 catcgcaacc atgctcgcgc tgctcaacct gctctatctg ctgttctgcg ccgtccagtt
    12001 cgcctatctg ttctcgacgg gcgaacgcgc gctgccggcg gggttcactt acgcggaata
    12061 cgcgcggcgg gggttctatg aactctgcct ggtcacgggg ctgaacatcg cctgcgtgct
    12121 gccgggcctc ttcatggttt gccccggggg aaggcggtcc tcgctcctgc tccgttccct
    12181 gcagacgctg ctcgtcgcgc agagcggcgt catcctggcc tccgccttca tgcgcctctc
    12241 gctgtatgaa gcggcctacg gctacacggc cacgcggctg ttcgcccgca cgatgatcct
    12301 gctgatggcg gcgacgctgg cgatgctggc ggcgcgcgtc tggctggagc ggtttccgtt
    12361 tgcaaaggcg tttctgctca ccgcgctcgc gctatacctc ggcctcaact atttcaacgg
    12421 cgaggccttc atcgcccgcc gcaacatcga ccgccacttg gccggcgcgg acctggatct
    12481 gccgtatctg cagcggctgt cgcacgatgc ggttcccgaa ctgatgcggc tgctggatag
    12541 tcccgacccg ctcattcgac aaggcgtgcg ggatcatctt gcgcaacggc ggcgcgcgct
    12601 cgccgacggc gaagcatggg tcagctggaa ccgagcgcgt cagcgggcgc gcgcgctgct
    12661 gcagaacgac gttccgcatg cggacccgat tcaacttcaa ccgacaggag acccgtcatg
    12721 aaaatcatcc gcgccgaggc catggggatg tgttttggcg tgcgcgacgc cctcggggtc
    12781 acgcgccgca tcgccgatcc cgcttccgtc acgattcacg gcgaactggt gcacaatccc
    12841 cgcgtggtgc gcgagctgtc ggcgcgcggg ttcacggtcc tggatgaaac ggagcggcgc
    12901 gacgcaacgc ccgcgacctc gcgggtcatg attaccgcgc acgggatcag cgaacgagaa
    12961 cgcaaacgcc tggaggagcg cggcc
//
LOCUS       DAOPCO010000087        12974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4789_length_12974_cov_5.247233, whole genome shotgun sequence.
ACCESSION   DAOPCO010000087 DAOPCO010000000
VERSION     DAOPCO010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4789_length_12974_cov_5.247233"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLS90_07900"
     CDS             <1..961
                     /locus_tag="PLS90_07900"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPK02367.1"
                     /translation="GSGMARRGGRVRLLVAGLALVLACRGEMVRGETRQRIELPFEGV
                     IHIHRTQSEPRELSIHLLLIDLATPGLRFMLTPANGPEAPGETTVETTRQFVARTGAQ
                     IGVNVSFYKPAPQLPFVDNLFLMVAQGDCYSGFGNGPAINLGRDNRATLLTPAAGGAP
                     DDCQPAQDVAPHVAFGGNERLIDGGRNVAAWEELHPRTAAGLTEDGRLMLAVVDGRQP
                     GRSAGMTTPELAELMLEYGAVEAINLDGGGSTTLVFADPDPRLVNVPVGIDDIAGTER
                     PVGGNLAIFVPYRDGAAEAERPMSEGDPRWYDGATTAPAERAR"
     gene            958..2061
                     /locus_tag="PLS90_07905"
     CDS             958..2061
                     /locus_tag="PLS90_07905"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPK02368.1"
                     /translation="MRGAAPAAIASSAPGRICLFGEHQDYLGLPVIAMAIDLRIEVRG
                     RRRDDGDATLRIELPDLGRRDAFNPNEEPVYRHGRDYLPAAANVLRRLGLSWPAGYDI
                     RVTSRIPINSGASSSSALQVAWVGFLLAAAGDARATDPLFVAEIAFQSEVAEFGSPGG
                     MMDQFSTALGGMIYLETRPPYRVERLPARDWAFVLIDSGEPKGTNEVLGRVRGGVEAG
                     LAALRAARGGAAVDVGTLTASEAAELEAGAAREMLESNLRSREITQEARRRLEQPACD
                     PREIGALLERHHAELAEHLRVSTERIDSLLRRAREMGAFGGKINGSGGGGGCFSLCPV
                     EAERVAAAIRADGYRVDVVRPAEGLRVEVGEMA"
     gene            2120..2866
                     /locus_tag="PLS90_07910"
     CDS             2120..2866
                     /locus_tag="PLS90_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPK02369.1"
                     /translation="MQRDYEADIRSVYQSILVDGDIAIDVGAHVGEHSIGILEKITPS
                     GHLYAVEPLPAKFREMSERIAHYYPSWRSNLTCYDCALSDRRGRDEFVVAVDAPWFSG
                     LKTRCYDVPTRLERIEVEVRMLDEVFADLPRLRYLKVDAEGGEYHILRGGRGLIERHR
                     PLVTFEFGGNSIGEYGITSQDMWDLWQALDYRLVDIDGAAMVDGDCFNRSVEEQRLWD
                     YIAIPAENEAMAGTVRAVLRGGLIRLKSAA"
     gene            complement(3172..3582)
                     /locus_tag="PLS90_07915"
     CDS             complement(3172..3582)
                     /locus_tag="PLS90_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02370.1"
                     /translation="MTTLNLALVCFSLELVLLALALILGHRPLHQRLTPARATAPAHP
                     SVAQAAARLCAVHESLMRQDRAIAARIAAGEDSTVLAEAAVGCRRTIMTVRKAAATLA
                     DQGAAAVPHCLEMLSGCEALAGTLGELVPDPPRA"
     gene            complement(3596..5377)
                     /locus_tag="PLS90_07920"
     CDS             complement(3596..5377)
                     /locus_tag="PLS90_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02371.1"
                     /translation="MRATLLLLFIGLPLLGAAPAPTGPALPSFAELAASADDPDPGTP
                     TGPSIASTEVSALSPAISWPGDAPPRVSQPTLSLEPADAAGPRILKLALPLAAAEAFE
                     AILWWRDDDGARQRIVLQPVAPGWLAAAVDHAWTDTVWELNATVIAYKGGSTVHHWEQ
                     TYLLTGMGQRLDLERSTDRLAANPAPDRADLLTSPTAGSSGRGLALDPHLALNRQRKP
                     AAAADHAISIPVLLIVLALLNVGLALVLGVAAVLARRLAAGGGAWLPRLRGGDVKTRL
                     REELAAAGLINPRDGQPMPLDAMAATIPMASPDSPGPQADLPPAAPAAPAPFPPEDLG
                     PDAEPLGAPEVYDAESPLPPAEDFPAEPLEAPAEFTDILGPAEDDDVVPPDDAPLPRI
                     PAAVPVRDASELTAANEVRGVLEDLVAAAEERAPELPLPAEPEEEASAPPTAIPASPT
                     ADEDTAPLPPPALDLTAVADLAEPPLPAAPVQTESPPDDSPPVDASAPPEAAADNSNG
                     DPDLGADAEHSRRVLDALKKRLSLRRKPKDPPAEPESPEAAEEELPEPLPSPLEPDPE
                     EEVVLREAASPEPDTEDLKFNFDDLSF"
     gene            6236..6688
                     /locus_tag="PLS90_07925"
     CDS             6236..6688
                     /locus_tag="PLS90_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK02372.1"
                     /translation="MLTQTSEIAIKALIFLECRGEGKPVAPREIARQMGASPTYLAKV
                     MGQLVKAGILRSQRGPQGGMMLARPPGEITLRQIVEATQGLIVPDYCQAIGDALGPVC
                     GFHRAMWEVRRNMLETMERWTLAELTAEPTPTGELAGNPACQMAFLDT"
     gene            6899..7864
                     /locus_tag="PLS90_07930"
     CDS             6899..7864
                     /locus_tag="PLS90_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HPK02373.1"
                     /translation="MARRPSWAWAIALTALIAAASAELANAQGEPVSSATPPIAAAPG
                     ADPRVESGTGDGPTNAAVAAAPADAISTATAAGAAGGIDPAAVNFLNKCASCHTVGGA
                     GIGNSPDLLPSTQWPRADLATAIERMEKNVGPLEAGEVEALTDLLRAPDVQARLEAAR
                     QEREARRQATAEPGLARTGEALFHGAQPLARRGMACAACHAAGGRGGTLAADLTHVYD
                     RMGETALRSAIENPSFPLMRPAYGARPLTSQEAAHLTAYLERAHASATDAQADPPAAA
                     AERGFGWAGLLLAGVAMSGAALGLRRKDHGGVRARLVRQATRSER"
     gene            7861..11493
                     /locus_tag="PLS90_07935"
     CDS             7861..11493
                     /locus_tag="PLS90_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HPK02374.1"
                     /translation="MSWITDLFQPEQRTWEDFYRNRWSYDKIVRSTHGVNCTGSCSWN
                     IYVKRGIVTWEMQALDYPQIDAKLPPYEPRGCQRGISCSWYVYSPMRIKYPTVRGVLL
                     DLWRAARKEHPNDPVAAWASIQDDPEKRRAIQRARGKGGFRRAHWEEVYEIMAAANIH
                     TVKRYGPDRLIGFSPIPAMSMISYAAGARFLQLMGGVCLSFYDWYCDLPPASPEIWGE
                     QTDVAESADWYKSKFIAVVGSNVLMTRTPDAHFLVEARHQGAKVVVFSPDFSQTSKVA
                     DEWLPLHQGQDGAFWMAVNHVILKEAYAERETAPFVEYVKRYTDAPYLVELEEEKEGA
                     RRPGRMLRAARVEPFAQAEHAEWKLLVLDGEGRLRMPMGSVGHRWQTTKGQWNLKQED
                     ALTGEPIDPLLSLHGWSRSRATIEIDDFSEGEPRALRREVPIHTIETVDGPRLVTTAF
                     DLMMAQYGVNRGGYGGEWPSGYDDASQPFTPGWQERFTGVAAENVVRFAREWTRTAEK
                     SGGRCMIIIGAGVNHWYHNNLIYRACIHALMLTGCIGKNGGGWNHYVGQEKLVPQASW
                     APIAFGLDWGGPPRLQNAPSWHYMHSDQWRYDRPFGDMCPVSETGHPLSRGHTADRQV
                     TAVRHGWLPCFPQFNRHNFAVVQEAREAGAQTDEEIRRHVVERLKSGELKFAMEDPDA
                     PECWPRVWYIWRGNAIQASAKGHEYFLKHYLGTHNNLVAEEEGAGHVEEVVWHDKVEL
                     GKMDLIVDLNFRMDTSALYSDIVLPAATYYEKDDLSSTDMHSYIHPLQAAVPPCWEAK
                     SDWAIFRELAWHTQRLGERHFPRPVKDIVATPLMHDTPAELAQPTFKDWSRGECEATP
                     GKTMPNLTLVERDYANVFMRFVSLGRKFRDGGLSVHGTHYDVSDVYDAYCERAPVENW
                     NGSGSFPSLREDRDVCNVILHFAAETNGELAWRAFVSESKKTGIDHTHLAEPMRGVRT
                     TFADICAQPRRVLTTPFWTGITNGGRTYAAYCQNVEERIPWRTLTGRQHFYLDHEAYR
                     AFGEHLATFKPRPPQAASRDLVQTPGDPRAITLNYLTPHGKWHIHSTFGDTLRMMTLS
                     RGCEPIWLNDRDAERAGIIDNDWVEAINDHGVVVTRACVSARIPKGLCFIYHATERTL
                     GVPKSPSRGNRRAGAHNSLTRARLKPLFMIGGYAQFTYAFNYWGPPGVNRDTWIQVKK
                     IEQPEW"
     gene            12025..12675
                     /locus_tag="PLS90_07940"
     CDS             12025..12675
                     /locus_tag="PLS90_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HPK02375.1"
                     /translation="MKTRTGAVRTVLAAVGLLVLAAPAARAHCDTLDGPVVGDAREAL
                     KQRDVTPVLKWVRAEDEAEIREAFDETLEVRELNETARELADRYFFETLVRVHRAVEG
                     APYTGLKPAGTEVAAGIRLADEALETGSVDEVVERLQAELEEGVGARLKRSHALRQHA
                     AESVAAGREYVEAYVEAIHYVEGLHGAIEGAAHGHGNADAGAHADGAAAEKKHGGH"
BASE COUNT         2230 a         3464 c         5382 g         1898 t
ORIGIN      
        1 tggaagcggg atggcgcggc ggggcggaag ggttcggttg ctggtcgccg ggttggcgct
       61 ggtccttgcc tgccggggcg agatggtccg gggcgagacg cgccagcgga ttgaacttcc
      121 gttcgaaggc gtgatccaca ttcaccgcac gcagagcgag ccgcgcgagc tgagcatcca
      181 cctgctgctg atcgacctgg cgacgccggg gctgcggttc atgctgacgc cggcgaacgg
      241 gccggaggcg ccgggggaaa cgacggtgga gacgacgcgg cagttcgtgg cgcggactgg
      301 cgcgcagatc ggcgtcaacg tctcgtttta caagccggcg ccgcagctcc cgttcgtcga
      361 caacctgttt ctgatggtcg cgcaggggga ctgctattcg ggtttcggca acgggccggc
      421 gatcaatctg ggacgggaca acagggcgac gctgctgacg ccggcggcgg ggggcgcgcc
      481 cgatgattgc cagccggcgc aggatgtggc gccgcacgtt gcgttcggcg ggaacgaacg
      541 gctgatcgac ggcggccgga acgtcgcggc gtgggaggag ctgcacccgc ggacggcggc
      601 ggggctgacc gaggacgggc ggctgatgct ggccgtcgtc gatggccgtc aaccgggccg
      661 cagcgccggg atgacgacgc cggagctggc ggagctgatg ctggaatacg gcgccgtgga
      721 ggcgatcaat ctggacggcg gggggtcgac gacgctggtc ttcgcggacc ccgacccgcg
      781 gctggtgaac gtgccggtcg ggatcgacga catcgcgggg acggagcggc cggtgggcgg
      841 gaatctggcg attttcgttc cgtatcgcga cggcgcggcg gaggcggagc ggccgatgag
      901 cgagggggac ccgcgctggt atgacggggc gacgaccgcg ccggccgagc gggcgcgatg
      961 aggggggcgg cgccggcggc gatcgcgagc tccgcgccgg gccggatctg cctgttcggc
     1021 gagcaccagg attacctggg gctgccggtg attgcgatgg cgatcgacct gcggatcgag
     1081 gtgcggggtc ggcggcgcga cgacggcgac gcgacgctgc ggatcgagct gcccgacctg
     1141 gggcggcgcg acgcgttcaa tccgaatgag gagccggtct accgccacgg ccgcgattac
     1201 ctgccggcgg cggcgaacgt gctgcggcgg cttgggctga gctggccggc ggggtatgat
     1261 atacgggtaa ccagccggat cccgatcaac agcggggcgt cgagttcgtc ggcgctgcag
     1321 gtggcgtggg tcgggtttct gctggcggcg gcgggggacg cgcgcgcgac tgatccgctg
     1381 ttcgtggcgg agatcgcgtt tcagtccgaa gtcgctgagt tcggttcgcc gggcgggatg
     1441 atggaccagt tcagcacggc gctgggcggg atgatctacc tggagacgcg cccgccgtac
     1501 cgggttgaac ggctgccggc gcgcgactgg gcgttcgtgc tgatcgactc gggcgagccg
     1561 aaagggacga acgaagtgct gggccgggtg cgcggggggg tggaggccgg gctggcggcg
     1621 ctgcgcgcgg cgcgcggggg cgcggcggtg gacgtcggga cgctgacggc gagcgaggcg
     1681 gcggagcttg aggcgggggc ggcgcgcgaa atgctggagt cgaacctgcg cagccgcgag
     1741 atcacgcagg aggcgcggcg acggctcgag cagccggcgt gcgatccgcg ggaaatcggc
     1801 gcgctgctgg agcggcatca cgcggaactg gcggagcatt tgcgggtttc gacggagcgg
     1861 atcgatagcc tgctgcggcg ggcgcgggag atgggggcat tcgggggcaa gatcaacggg
     1921 tcggggggcg gcggcgggtg cttttcgctc tgcccggtcg aggcggaacg cgtagcggcg
     1981 gcgatccgcg ccgatggtta ccgggtcgac gtcgtgcggc cggcggaggg gttgcgggtg
     2041 gaggttgggg aaatggcgtg aaaaaggcag cccgccggga atccggcggc gggcgcaact
     2101 ccctggaggc gacgggctca tgcaacggga ttacgaagcc gatatccgga gcgtttacca
     2161 atcgattctg gtcgacggcg acattgcgat cgacgtcggc gcccacgtgg gcgagcattc
     2221 gatcgggatt ctggagaaga tcacgccgag tgggcatttg tacgcggtgg agccgctgcc
     2281 ggccaagttc cgggagatgt ccgagcggat cgcgcattat tatccgagct ggcgaagcaa
     2341 cctgacgtgt tacgactgcg cgctgagcga ccggcggggg cgcgatgagt tcgtcgtcgc
     2401 ggtcgatgcg ccgtggttca gcggactgaa gacgcggtgt tacgacgtgc cgacacggct
     2461 ggagcggatc gaggtcgagg tgcggatgct ggacgaggta ttcgccgatc tgccgcggct
     2521 gcgctacctg aaagtggatg ccgagggcgg ggagtatcac attttgcggg gcgggcgcgg
     2581 gctgattgag cgccaccggc cgctcgtcac gtttgaattc ggcggcaatt cgattgggga
     2641 atatgggatt accagccagg acatgtggga tctgtggcaa gcattggatt atcggttggt
     2701 cgatattgac ggcgcggcga tggtcgatgg ggattgtttc aaccggagcg tcgaggagca
     2761 gcggttgtgg gattacattg cgattccggc ggaaaatgag gcgatggcgg ggacagtccg
     2821 ggcggtcttg cggggggggc tcattaggtt aaaaagcgcg gcgtgaggta agggaaattt
     2881 ttagttggga taaaatccgt cggatcggac agctcagccg gattttggaa ctgccggggg
     2941 tttgaccggc gaggacgccg gacacgacag cggtagcggc gctgtggttg gttgtgttcc
     3001 cgccatccct tgccaggggt ttggggcgtt cggggggcga cgcagggttc cggcctgcgg
     3061 ccgtcaccct gcgcgttatt ccggtcgtcc gctttgcgga ctggtcgtga ggccggcagg
     3121 ggttgaactg acgaaggtct ggtttcgaga gtcgaggacg agggatgagc tctatgcgcg
     3181 gggagggtcg gggacgagtt cgccgagggt gccggcgagg gcttcgcagc cgctgagcat
     3241 ttcgaggcag tgcgggacgg cggcggcgcc ctgatcggcg agggtcgcgg ccgccttgcg
     3301 gacggtcatg atcgtgcggc ggcagccgac ggcggcctcg gccaggaccg tggaatcctc
     3361 gccggcggcg atgcgcgcgg cgatcgcccg gtcctggcgc atgagcgatt cgtggacagc
     3421 gcacaggcgg gcggccgcct gggcgacgga cgggtgagcg ggcgccgtcg cgcgggcggg
     3481 ggtcagccgc tggtgcaacg ggcgatggcc cagaatgagc gccagcgcga gcaggacgag
     3541 ttcgagggag aaacagacga gggccaggtt gagcgtggtc atcgtgcgcg gcctttcaaa
     3601 aactaaggtc gtcaaaattg aatttgagat cttcggtgtc gggctcgggg gaggccgcct
     3661 cgcgcagcac gacttcttct tccgggtccg gttcgagcgg actgggcaac ggttcgggca
     3721 gttcttcctc ggcagcttcg ggggattccg gttcggccgg ggggtccttc ggcttgcggc
     3781 gcagcgagag gcgcttcttg agcgcgtcga ggacgcgacg ggagtgctcc gcgtcggcgc
     3841 ccaggtcggg gtcgccgttg gagttgtcgg cggccgcctc gggcggggcc gatgcatcga
     3901 ccggcggcga gtcgtccgga ggggattcgg tttggaccgg cgcggcgggg agcggcggct
     3961 cggccaggtc ggcgacggcg gtcaggtcca gagcgggtgg cggtagcggg gcggtatcct
     4021 cgtcggccgt gggggaagcg ggaatggcgg tcggcggcgc ggaggcctcc tcctccggtt
     4081 cggccggcag cggcagctcg ggggcgcgtt cctcggcggc ggcgaccagg tcctccagga
     4141 cgccgcggac ttcgttggcg gcggtcaatt ccgaggcatc cctgacgggg acggcggccg
     4201 gaatgcgggg gagcggggcg tcgtcgggcg gcacaacatc gtcgtcctcg gcagggccca
     4261 gaatatcggt gaattcggcg ggagcctcga gcggttcggc cgggaagtcc tccgcagggg
     4321 gaagcggcga ttcggcgtca tatacctccg gggcgccgag cggttcggcg tcgggaccga
     4381 ggtcttcggg agggaatggc gccggcgcgg cgggtgcggc gggcggaagg tcagcctggg
     4441 ggccgggtga gtcgggagag gccatcggga tcgtggccgc catggcgtcg agcggcatcg
     4501 gctgaccgtc gcgggggttg atcagaccgg cggcggcgag ttcctcgcgc aggcgggtct
     4561 tgacgtcgcc gccgcgcagc cgggggagcc aggcgccgcc gcccgcggcc aggcggcgcg
     4621 ccaggaccgc cgcgacgccc aggacaagcg ccaggccgac gttgagcagc gccagaacga
     4681 tcagcaggac ggggatcgag atcgcgtgat cggcggccgc ggcgggcttg cgttgccggt
     4741 tgagcgccag gtgcgggtcc agggcaaggc cgcggccgga cgaaccggcg gtgggagacg
     4801 tgaggaggtc ggcgcgatcc ggcgccgggt tggcggcgag ccggtcggtg gagcgctcca
     4861 gatcgagccg ctggcccatg ccggtcagga ggtaggtctg ctcccagtgg tggacggtgc
     4921 tgccgccctt gtaggcgatg acggtggcgt tgagttccca gacggtgtcg gtccaggcgt
     4981 ggtcgacggc ggcggcgagc cagccggggg cgaccggctg aaggacaatg cgctggcggg
     5041 cgccgtcgtc gtcgcgccac cagaggatgg cttcgaaggc ctcggcggca gcgaggggca
     5101 gagccaattt gaggatccgg ggacccgccg cgtcggctgg ttcgaggctc agggtgggtt
     5161 gtgaaacgcg cgggggggcg tcgccaggcc atgagatggc gggcgagaga gcggaaactt
     5221 cggttgatgc gatggatggg ccggtgggag tcccgggatc gggatcgtcg gccgatgccg
     5281 cgagttcggc gaacgagggc agggcggggc ccgtgggggc cggcgcggcg cccagcagcg
     5341 gcagaccgat gaagaggagc agcagcgttg cgcgcatgga gcggttcttc actcgaggaa
     5401 aatccggatg cttgcggcgc aaggatcctg ttggtcgtat cggcgcgcgg gggagcgaaa
     5461 tcaaccggcg cgtgggggat tgcgacgatg aagaggatga agacaggaaa gaattctgga
     5521 agtcgtcgtc atagtgctgt ggacagcgtg gataagcggg tcaagtcatg ttgtttcaag
     5581 ggttttgggg tcttggcgga tcgtgggaga attcagaatt cactcacagg aacggcgcgg
     5641 ggcggggagc gatggcgcgt gagaggggtg gggtgaggcg ggcctggggg acaggctgtg
     5701 gaaaaccgga gcccggctgg cgggggtgaa atgaggcctg gaaagcgggg aagcagggtc
     5761 agttccgagc ggcgggatgc cggagttatc cacagaacgg gtgccgggcg gcgataaccg
     5821 acggcgacat cggctttatc tagataagtc ctttaaatca aatggcttaa gtagattttg
     5881 aggggcttag aaaaaattgg cggaggcgtg gaagggggag ttattcacag ggcgcgaaac
     5941 ggctgtggat aacttccaaa gccgcgtcaa atcggccttt tcggcgtgtc ggaggccacg
     6001 aagagacccc gtgccagacg acttcttcac atgggggggc cggcgggcgg ggatacgggg
     6061 atagttcctg agcaggttcc ggggagtatg aaccacggag gacgccacgg aaagaagcac
     6121 gggatcacga aagggacgaa aagtgggggc tggatttttt tttgattaag gggattgacg
     6181 gcggggacga attaatgata tgtgatatct tgaatatccg gaatggagcg cctggttgct
     6241 cacacagact tcggaaatcg ccatcaaggc cctgattttc ctcgaatgcc ggggggaagg
     6301 caagccggtc gcgccgcgcg agatcgcgcg gcagatgggc gcgtcgccga cgtatctggc
     6361 caaggtgatg gggcagttgg tcaaggcggg gatcctgcgc tcgcagcggg ggccgcaggg
     6421 ggggatgatg ctggcgcggc cgccggggga gatcacgttg cggcagatcg tggaggcgac
     6481 gcaggggctg atcgtgccgg actattgcca ggcgatcggc gacgcgctgg gtccggtgtg
     6541 cgggtttcac cgggcgatgt gggaggtgcg gcgcaacatg ctggagacga tggagcgctg
     6601 gacgctggcg gagctgacgg ccgagccgac gccgacgggg gaactggcgg ggaatccggc
     6661 gtgccagatg gcgttcctgg atacgtaaag caccaactga agatgaaatg ggatcacgaa
     6721 gggcacgaaa gggcacgaaa tggaatggat ttcgagagcc gaggatgacg gacgagagcc
     6781 gagagccgtc taatataaga catcggtatt agattataat aaaacttttt tagcctaaag
     6841 aaacggaagc gacatggcgt cagaaacatc ccgagcgggg ggccgattgc ggcccggcgt
     6901 ggcgcggcga ccgagctggg cgtgggcgat tgcgctgacg gcgctgatcg ccgcggccag
     6961 cgccgagctg gcgaacgccc agggggaacc ggtgtcttcc gcgactccgc cgattgccgc
     7021 ggcgcctggc gccgatccga gggtagaaag cggaaccggc gacgggccaa cgaacgccgc
     7081 cgtagcggcg gcaccggcgg acgcgatttc aaccgccacg gcggcgggag ccgcgggggg
     7141 gatcgatccg gccgcggtga acttcctgaa caagtgcgcc agttgccata cggtcggcgg
     7201 ggcggggatc gggaactcgc cggacctctt gccgtcgacg cagtggccgc gggccgacct
     7261 ggcgacggcg atcgagcgaa tggagaaaaa cgtggggccg ctggaggcgg gcgaggtgga
     7321 ggcgctgacc gatctgctgc gggcgcccga cgtgcaggcg cggctggaag cggcgcgcca
     7381 ggagcgcgag gcgcggcgcc aggcgaccgc cgagccgggc ttggcccgaa ccggggaggc
     7441 gctgttccac ggcgcacagc cgctggcgcg gcgcggaatg gcctgcgcgg cgtgtcacgc
     7501 ggcggggggg cggggcggga cgctcgcggc cgacctgacg cacgtgtatg accggatggg
     7561 ggagacggcg ctgcgttcgg cgatcgagaa tccgagtttt ccgctgatgc ggccggcgta
     7621 tggcgcgcgc ccgttgacga gccaggaagc ggcgcacctg acggcatatc tggaacgggc
     7681 acacgcgagc gccacggacg cgcaggccga tccgccggcg gcggcggcgg aacgcgggtt
     7741 cggctgggcg gggctgctgc tggcgggggt cgcgatgagc ggggcggcgc tggggctgcg
     7801 gcggaaggat cacgggggcg tgcgggcgcg actggtgaga caggcaacga ggagcgaacg
     7861 atgagttgga tcaccgacct attccagccg gagcagcgca cgtgggagga cttctaccgc
     7921 aaccggtgga gctacgacaa gatcgtccgc tcgacgcacg gggtgaactg cacaggatcg
     7981 tgcagttgga acatctacgt caagcggggg atcgtgacgt gggagatgca ggcgctggat
     8041 tacccacaga tcgatgcgaa gctgccgccg tatgagccgc ggggctgcca gcgcggaatt
     8101 tcgtgttcgt ggtatgtcta cagtccgatg cggatcaaat acccgacggt gcgcggggtg
     8161 ctgctggacc tgtggcgggc ggcgcgcaag gagcatccga acgatccggt ggcggcgtgg
     8221 gcgtcgatcc aggacgatcc ggagaagcgg cgggcgatcc agcgggcacg cggcaagggg
     8281 ggcttccgcc gggcgcactg ggaggaagtg tatgaaatca tggcggcggc gaacattcat
     8341 acggtgaagc gttatgggcc ggaccggttg atcggatttt cgccgattcc ggcgatgtcg
     8401 atgatcagtt acgccgccgg cgcgcggttt ctgcagctca tggggggcgt ctgcctgagc
     8461 ttctacgact ggtattgcga tctgccgccg gcgtcgccgg agatctgggg cgagcagacc
     8521 gacgtggcgg agagcgcgga ctggtacaag agcaaattca tcgcggtggt ggggtcgaac
     8581 gtgctgatga cgcggacgcc ggacgcgcac tttctggtgg aggcgcgcca ccagggggcc
     8641 aaggtggtcg tcttctcgcc cgacttcagc cagacgagca aggtggcgga cgagtggctg
     8701 ccgctgcacc aggggcagga cggcgcgttc tggatggcgg tgaaccacgt gatcctgaag
     8761 gaggcgtatg ccgagcgcga gacggcgccg ttcgtggagt atgtgaagcg gtatacggac
     8821 gcgccgtatc tggtggagct ggaggaggag aaggaggggg cgcggcggcc ggggcggatg
     8881 ctgcgcgcgg cgcgggtcga gccgttcgcc caggcagagc atgccgagtg gaaactgctg
     8941 gtgctggacg gggagggccg gctgcggatg ccgatgggga gcgtcgggca ccgctggcag
     9001 acgaccaagg ggcagtggaa cctgaaacag gaagatgcgc tgacgggcga gccgatcgat
     9061 ccgctgctga gcctgcacgg gtggagccgg agccgggcga cgatcgagat cgacgacttc
     9121 agcgaggggg aaccgcgggc gctgcggcgc gaagtgccga tccacacgat cgagacggtc
     9181 gacgggccgc ggctggtgac gacggcgttt gacctgatga tggcgcagta tggggtcaac
     9241 cgcgggggct acggcggcga gtggccgagc ggttatgacg acgcgagcca gccgttcaca
     9301 ccgggatggc aggagcggtt cacgggcgtg gcggcggaga acgtggtgcg gtttgcgcgg
     9361 gaatggacgc ggacggccga gaaatcgggg ggccggtgca tgatcatcat cggcgccggc
     9421 gtgaaccact ggtatcacaa caacttgatc tatcgcgcgt gcattcacgc gttgatgctg
     9481 accggctgca tcgggaagaa cggcggcggg tggaaccact atgtgggcca ggagaagctg
     9541 gtgccgcagg cgagctgggc gccgatcgcg ttcgggctgg actggggggg gccgccgcgg
     9601 ctgcagaacg cgccgagctg gcattacatg cacagcgacc agtggcgcta cgaccggccg
     9661 ttcggcgaca tgtgcccggt gagcgagaca gggcatccgc tgagccgggg ccacacggcg
     9721 gaccggcagg tgacggcggt gcgccacggc tggctgccgt gttttccgca gttcaaccgg
     9781 cacaacttcg cggtggtgca ggaggcgcgg gaggcggggg cgcagacgga tgaggagatc
     9841 cggcgccacg tggtggaacg gctcaagtcg ggggaattga agttcgcgat ggaggatccg
     9901 gacgcgccgg agtgctggcc gcgggtgtgg tacatctggc gcggcaacgc gatccaggcg
     9961 tcggcgaagg ggcacgagta cttcctgaag cattacctgg ggacgcacaa caacctggtg
    10021 gcggaggaag aaggggcggg gcacgtggag gaggtcgtct ggcacgacaa ggtcgagctg
    10081 ggcaagatgg acctgatcgt ggatttgaat tttcggatgg atacgtcggc gctctacagc
    10141 gacatcgtgc tgccggcggc gacatattat gagaaggacg acctgtcgag caccgacatg
    10201 cacagttaca tccacccgct gcaggcggcg gtgccgccgt gctgggaggc gaagagcgac
    10261 tgggcgattt tccgggagct ggcgtggcac acgcagcggc tgggggaacg gcattttccg
    10321 cggccggtga aggacatcgt cgcgacgccg ctgatgcacg acacgccggc cgagctggcg
    10381 cagccgacgt tcaaggactg gagcagagga gagtgcgagg cgacgccggg caagacgatg
    10441 ccgaacctga cgctcgtcga gcgcgactac gcgaacgtct tcatgcggtt tgtgtcgctg
    10501 gggcgcaagt tccgcgacgg ggggctgagc gttcacggaa cgcactacga cgtgagcgac
    10561 gtttacgatg cgtattgcga gcgggcgccg gtggagaatt ggaatggttc ggggagtttc
    10621 ccctcgctgc gcgaggaccg cgacgtgtgc aacgtgatcc tgcacttcgc ggcggagacg
    10681 aacggcgaac tggcgtggcg cgcgttcgtg agcgagagca agaagaccgg gatcgaccac
    10741 acgcacctgg ccgagccgat gcggggggtg cgaacgacgt tcgccgacat ctgcgcgcag
    10801 ccgcggcggg tgctgaccac gccgttctgg accgggatca ccaacggggg gcggacgtac
    10861 gcggcctact gtcagaacgt cgaggaacgg atcccctggc ggacgctgac gggccggcag
    10921 catttctacc tggatcacga ggcctaccgg gcgtttgggg agcacctggc gacgttcaag
    10981 ccgcggccgc cgcaggcggc gtcgcgcgac ctggtgcaga cgccgggcga cccgcgggcg
    11041 atcacgctga actacctgac gccgcatggg aagtggcaca tccactccac gttcggggat
    11101 acgctgcgga tgatgacgct gtcgcgcggg tgcgagccga tctggctgaa cgaccgcgac
    11161 gccgagcggg cggggatcat cgacaacgac tgggtggagg cgatcaacga tcacggggtg
    11221 gtggtgacgc gggcgtgcgt gagcgcgcgg atcccgaagg ggctgtgttt catctatcac
    11281 gcgacggagc ggacgctggg ggtgccgaag agcccgagcc gggggaatcg gcgcgccggg
    11341 gcgcacaaca gcctgacgcg ggcgcggctg aagccgctgt tcatgatcgg cggctacgcg
    11401 cagtttacgt atgcgttcaa ctactggggg ccgccggggg tgaaccggga tacgtggatt
    11461 caggtgaaga agattgaaca gccggagtgg tgaaccgccg aagcgggagc gcgagggaaa
    11521 gaagccacgg acggcgggga ggaaaggcga gggatttacg gagcaggccc gaaggcggac
    11581 aacaagtaac agcctgacgc caatgggacg gggctgacat gagtatgccg gcgttggcgg
    11641 ggaaaccgtt gaaacggtag ggaagaaggg ggcgcgcggt caccgggccg aggcccggtg
    11701 cgaataacag ggcccccgag ggggcccccg aaaagcccgc tggcgcgggc tcgcgatgcg
    11761 atcgcgatcc cgatagcgac ggcgatgcgc agtgaggccg gcaaggatgc cggcggtccc
    11821 aggcggtggc gggtattcga gagccgagga cgagggacga gggacgagag ccgaattttg
    11881 gggggggccg ttgggggcga ggacgcggtg atcatgaggc ggaggtagaa cgtaaggaga
    11941 aatattggga cgggccgttg gggcgaatac gaagacagcg gacggcagac gagggacgac
    12001 attttagtga agggaacggg aaagatgaaa acgaggacgg gggcggtgag aacggtgctg
    12061 gcggcggtgg ggctgctcgt gctggcggcg ccggcggcgc gggcgcattg cgacacgctg
    12121 gacggaccgg tggtgggcga cgcgcgcgag gccctgaaac agcgggacgt gacgccggtg
    12181 ctcaagtggg tgcgggcgga ggatgaagcg gagatccggg aagcgttcga tgagacgttg
    12241 gaggtgcgcg aattgaatga gacggcgcgc gagctggccg accgctactt cttcgagacg
    12301 ctggtgcgcg tgcatcgggc ggtggaaggc gcgccatata cgggactcaa accggccggg
    12361 acggaggtcg cggcggggat tcggctggcc gacgaggcct tggaaacggg gagcgtcgat
    12421 gaggtcgtgg agcggctgca ggccgagctg gaggaagggg tcggcgcgcg gttgaagcgg
    12481 tcgcacgcgc tgcggcaaca cgcggcggag tcggtcgcgg ccggtcgcga atacgtcgag
    12541 gcgtacgtcg aggcgatcca ttacgtggaa gggctgcacg gagcgattga aggcgcggcg
    12601 cacggccacg ggaacgcgga cgcgggagcg catgccgacg gggcggcggc ggagaagaag
    12661 catggaggcc attagaaata gcacggaggg gaggaggagg gcacggagga gaggggagcc
    12721 tgacgccaat gggacggggc tgacatgggt ttgccggcgt tggcggggaa accgttgaaa
    12781 cggtagggaa gaagggggcg cgcggtcacc gggccgaggc ccggtgcgaa taacagggcc
    12841 cccgaggggg cccccgaaaa gcccgctggc gcgggctcgc gatgcgatcg cgatcccgat
    12901 agcgacggcg atgcgcagtg aggccggcaa ggatgccggc ggtcccaggc ggtggcgggt
    12961 attcgagagc cgag
//
LOCUS       DAOPCO010000088        12956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4794_length_12956_cov_5.064956, whole genome shotgun sequence.
ACCESSION   DAOPCO010000088 DAOPCO010000000
VERSION     DAOPCO010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12956
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4794_length_12956_cov_5.064956"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..2467)
                     /locus_tag="PLS90_07945"
     CDS             complement(131..2467)
                     /locus_tag="PLS90_07945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02376.1"
                     /translation="MEHSPTHPPAVARPPGEGGARPRRGAGALLESLRRRRSHAPAHL
                     LALLLYMMLTAVATWPLVTRPHRLLLGTPGDSMLWAWNFSWTRRALLADHQLPFFVDY
                     FYRPFDISMLHYTHSFFYGLLYTLGAFVYENPYFWSNLFLMGSFVVAGLGTYALARRV
                     AGDTPGALLAGVIYAFSSFRMVRATGHLNILSTELIPWFLYFLLRGARENRPRHFVAA
                     GLCLAASAWNDYYNTLFLLLFSVLFILLAPVLRLNHGWSARQRWRGLRAGAVLTLILL
                     APLVWGIWVTVHTTELSSRASHDQYPVGVLNYLLPSPGHTLLSRWLFPLYHFGIKAAE
                     GEGTVFFGYVAIVLMLAAVVGLRRRPPEFKFLMICMLVFLFLSFGSPMSDPDVGHLWK
                     VSMPYDWLLRIPFFKDFRAPARFGGMVALCVAVLAACGFRLLTARRGRGERRLWAAGL
                     IALVMLENLHAPFPYTYDALDPERVPHELNAIIRADPVGGAVLTLPFSHENFPAVWNQ
                     TFHHKPIITGIQSRPTESQIQYYTRIDVHDWFHADPQYREVAFDPLAPPQVRNYFGPP
                     GRRAWDELEKSWTREAILRFCYFFDLRYLLIPPSDLPTDVKGRLRKELFPIEREFESG
                     EAWLALLEKQREALPIVIEAGSAADHFYFTENFAEAAGGCRWIVGRQGQVTFRLDAPR
                     DLSISIGTGYPTHLLTAKQTMKVSLGGETFLDIKLPPDWSTHEFRVPARVVRPGYNRI
                     LIEAGYAPSPADADLGLDDRPLSIMIGRITIAEAPPAG"
     gene            2691..3581
                     /locus_tag="PLS90_07950"
     CDS             2691..3581
                     /locus_tag="PLS90_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HPK02377.1"
                     /translation="MGSKPWTTFLILRAMRPRQWMKNLLLFAGLFFSRNLLDPLSVKR
                     ALAGFVIFCGLSGAVYLLNDLIDAPRDRLHPRKRHRPIAAGQLAPRDAVLAIILLLAA
                     GLGAALWLSSYFWICCVAYLVLMLSYTTILKEIFLIDTLIIALGFIIRAVSGVIVLRT
                     PDNQVDLTSWFVICVLFLSLLLAFCKRRSELVNLEGSAIEFRPVLSLYSLRMMDSVIA
                     VCAAGCILSYTLYATHMEELWLMLSTLPFVLYGIFRYLYLVYNSDVTEAPEHALASDP
                     PLLGCVFLWGCSLVLVYFPA"
     gene            3605..4408
                     /locus_tag="PLS90_07955"
     CDS             3605..4408
                     /locus_tag="PLS90_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02378.1"
                     /translation="MPSRALLLGALCLLFYPDAAAQPPAESLLRERPAPASYPDPIVP
                     WLGLPSDAPPILHYRHDIRIGPPGAGLSIRESRWQADRLKLLGFGGSLGLVGVGTVSA
                     EAPLDLATTVTLETIDLNAVLRYLQLPRAEEIEGRVSGTVHLSLLGREWRRIDVALHA
                     PAGTVRIHRRLIADVLTTYLSEQLSREEIYASLDQAYRGIEMIPFDAVRVNGRFDQQT
                     LNLRVPLRNEILNLDLEPRIERAILWDLWDLLRRTGFEELEDIEWRTRR"
     gene            4624..6396
                     /locus_tag="PLS90_07960"
     CDS             4624..6396
                     /locus_tag="PLS90_07960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02379.1"
                     /translation="MTVPPKHKPFGLRHGVWLALLAAGCVGFTVAQWSRGYVDFGDGN
                     YMYIGMRIAEGVVPYRDILAPQPPCHLFMGALIYKLSQAFRPETPVYFFRGYSVALRL
                     LTLLLVVALAMRAWGRPSTALVAGAIYLWFPIGFWWSFAFQSEPLEIVFLLAMAWCAL
                     RGTRAGDIGTGIFAALAALTNATAAPFLLVLIIFMLLHAPGRALRMAAPCLLLAGLVT
                     LALHIWTEGAFLDVVVFKQVGTYPGGLGSPQFLNYARGKLLDQGGKVLALEGAFWVIA
                     LIAVPRYFRSSPLPPMARAGLTWFLLATMTAILYVTKGGTADYIFSLSEPAVAILAAG
                     ELVAWARRWKTRAAPEIGGGLSIRSIPFALPKLAAAWMLAVLALGPGLNFHALLWDQR
                     AYELSERGTPDRPGVRLVRRIIQEHSRPRDFILAPPYYAVDSDRRLWGEFSELFIWNI
                     TYLNDLRAGLREGTGWRKMREIGEALRAQQIPIVILEMGQTGMSREVMLALQDRYVPL
                     RPDRRRFLLHTLNTRLGFFIPAPPLEDETAAAERIRQWELFKLELLEVFGPGAANDQF
                     GSWYAERPAGPGASSGEAPPREPA"
     gene            6393..6950
                     /locus_tag="PLS90_07965"
     CDS             6393..6950
                     /locus_tag="PLS90_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK02380.1"
                     /translation="MNEPYDPHSASTDEELLPVVDEHDRPIGVRRRREIHRLGLRHRS
                     VQVCLVDPAGRIWLQKRSRRKDTFPGWWDMAVTGHVEPGETYAEAAIRELREELGLAA
                     QPRWIGRTAAAALTGWEFQEHYLLRWNGPITDFNREEIDELRPFRSDEIEQAARANHP
                     DLRLTPGVIYILPHLLAALKPEQTP"
     gene            6947..9850
                     /locus_tag="PLS90_07970"
     CDS             6947..9850
                     /locus_tag="PLS90_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK02381.1"
                     /translation="MNRPRVETGTGRAADDAAARRGGAHEPLTIRRDSFYRLEWLVLL
                     TCLAAVILVYPLRLQDAWLGQLYRWWDRSDGFWSRWTHAQFGAYLSFFYSPLILKGVI
                     AGVGAFIFCGLALARGLLGPHLRCGTIVERPQSPWAWCSVALLLGWVLASGLWSPTPA
                     QARDAAWFVAPFGLLLFLLLRRGLLRQEARWLAIALVSLGAIVMAVMLMQALPGLSEL
                     TFRVMTRFDDFRNLYGSLMGHNTAVASLLLMTLFPALALAAGARTRRRRLALGLYLVA
                     LVYGLIVTQSRAIWLIGPPLIVGQFIALLRATRSERQRRRLRGLPTLLLAIAALAILT
                     QVIRKPWNPFFVRAYTLSERLRDLGPDALRRESRLRLNVIGATLVPDRPLLGHGLAAF
                     QYVYPRRQGEYFSRHPESRLGRTEKRSHMAHNEYLQVAIEQGLIGLALLITVLAEVAR
                     RGGRRGRRLRGRSRLLHEAFGWSSLGFLLHAAVDFPFHIPQLLVPGLLCVAAWSGTGG
                     LIRRVGGDDPGRALPAGRSNFRWPAFARLLATMLLFFAVPLAGLPLVRRLQADVAYNE
                     GGAYLTRYDLFGQSWPPEEALANFELAAQSLREALRLQPSHSFAWQKLSQAQWLGGLM
                     KARMAALRQADGAPAADLTRAALDDMKAALGCLFKAAEGLDYHYLPYQRGRIYQSLYR
                     VTGDPRYQQLYVEGLEQALAYSDVFVDGLHELAEALARNPATRPRAMELRRRIIQFWP
                     DHFVRWYVDPAYELIANKRYQAAARELGWIVDLWADRDPWVLIEAALAHLRAGDRDRA
                     LELIRQLRERDLPTYHGAGGHLLEPLLLGQWAQAYEALASIMPPDAPSRAEMKVVEHE
                     LQRRLGRTTEPSRYPPPEGVAADAWQRLVAETRPMVLLHLFNDPERAEQALRDRLALP
                     GPPPAVDFWLEAIYIARARGNAELGREALERAAALDPRHPALLALRSTPDEEP"
     gene            10004..11026
                     /locus_tag="PLS90_07975"
     CDS             10004..11026
                     /locus_tag="PLS90_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02382.1"
                     /translation="MNRVKPPTTEASQTRILIVDDEPMLRSVVQDFLALLGYQKYWLA
                     GDGREALEILRAHPIDVMLSDIRMPEMELEELLTIVNDEFPGLVVIATSGYSDFESAR
                     NILCRGAHDFLEKPLNLDALEQSIGWVIRRRRILDEVAEQFGAGPTGDEAWRRLDHLC
                     DTLTDAGRTFPRALLHCVRTYQLSRRLDLPLSEEEHQELALAILLHEIGASLQVHSIC
                     ALPRLLDEEELALVHHQSRHSARLVGQVLDRPPLLAMIADHVHWREISALDPNANAQT
                     RLTVWLGLLNLLDGCLNSRADRGPIPIDQLRHSLQRRARQSPLAAVDHLLAKWPVVER
                     FYASSL"
     gene            complement(11198..11623)
                     /gene="msrB"
                     /locus_tag="PLS90_07980"
     CDS             complement(11198..11623)
                     /gene="msrB"
                     /locus_tag="PLS90_07980"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015487079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPK02383.1"
                     /translation="MEGCDVRTNEEWKQALTPEQYYVTREKGTERPFTGEYVHHKEAG
                     TYHCVCCGEPLFTSEAKFDSGTGWPSFFQPISGERVKELPDDSLGMRRTEVVCGKCDA
                     HLGHVFEDGPAPTGLRYCLNSASLTFQRASEGETPSAQP"
     gene            complement(11857..12393)
                     /locus_tag="PLS90_07985"
     CDS             complement(11857..12393)
                     /locus_tag="PLS90_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02384.1"
                     /translation="MSSGVSSSGSQPTRPQGTGLQGLITEMRAIQETQETETSRLRQR
                     LSWLESRRQEFYNLVASDNGSVDTRRKLEETDRQIERMQSTLNRAESDLKQSLLEIRQ
                     RIIALLNEEMALLEEQLKELRRRKNEIHHELLPEATARVTRLHEEESQLDQQIEEVVK
                     RLSALANFDVSAPNTSRS"
BASE COUNT         2023 a         4626 c         3981 g         2326 t
ORIGIN      
        1 gtgggcaaca ctttgacgcg gaaaacgatc tgctaacggc cacccatggg atcaaactgg
       61 gagccaactg tcccatggag atcgtatgct ttcatgccca gcagtgcggc cgacgacggg
      121 tgcggaggca tcagcccgcc ggcggcgctt ctgcgatcgt gatccgcccg atcatgatcg
      181 acagcgggcg gtcatcgagg ccgagatcgg cgtcggccgg cgagggagcg taacccgcct
      241 cgatcaggat gcgattgtag ccgggccgga ccacgcgcgc cggaacgcga aattcatggg
      301 tcgaccagtc gggcggcagc ttgatgtcca ggaacgtctc ccccccaagc gaaaccttca
      361 tcgtctgctt cgcggtgagc agatgggttg gatagccggt tccgatggaa atcgacagat
      421 cgcgcggagc gtcgagccgg aacgtgacct gcccctgccg tccgacgatc cagcggcagc
      481 cgccggccgc ctcggcgaag ttctccgtaa agtaaaaatg gtccgcggcc gagcccgcct
      541 cgatcacgat cggcaacgct tcccgctgct tctccagcag cgccagccag gcctcgccgc
      601 tctcgaattc ccgctcgatg ggaaacagct ccttgcgcag ccgacccttc acgtcggtcg
      661 gcaggtcgct cggcggaatc aacagatagc gcagatcgaa aaagtaacag aaacgcagga
      721 tcgcctcgcg agtccaggat ttttccagtt cgtcccacgc gcgccggccg ggaggaccaa
      781 agtagttcct gacctgcggc ggcgccaacg gatcgaacgc cacctcgcga tactgcgggt
      841 cggcgtgaaa ccagtcgtgg acgtcgatcc gagtgtaata ctggatttgc gactcggtcg
      901 gtcgcgactg aatcccggtg atgatcggct tgtgatgaaa cgtctggttc cagacggccg
      961 ggaaattctc atgcgagaac gggagggtca agaccgcgcc gccaacggga tcggcgcgga
     1021 tgatcgcgtt cagttcatgc ggcacgcgct ccggatcgag cgcgtcatac gtgtagggga
     1081 atggggcgtg caggttctcg agcatcacga gggcgatcag gccggcggcc cacaggcgtc
     1141 gttcgccgcg cccccggcgg gcggtgagca accggaatcc gcaggccgcc agcacggcga
     1201 cgcacagcgc gaccatcccg ccgaatcgcg ccggcgcgcg gaagtccttg aagaacggga
     1261 tccgcagcag ccagtcgtac ggcatcgaaa ccttccacag gtggccgaca tcgggatcgc
     1321 tcatcggcga gccgaacgag agaaacagga agacgagcat gcaaatcatc aggaatttga
     1381 actcgggcgg tctgcgcctc aaccccacca ccgccgccag catcagcacg atcgcgacgt
     1441 agccgaagaa gacggttcct tcaccctccg ccgccttgat gccgaagtga tacagcggga
     1501 acagccagcg cgacagcaac gtgtggcccg gcgacggcag caggtagttc agcacgccga
     1561 cggggtattg gtcgtgcgag gcgcggctgc tgagttcggt cgtgtgcacg gtgacccaga
     1621 tgccccagac cagcggcgcc agcaagatca gcgtgagcac ggcgccggcg cgcagcccgc
     1681 gccagcgctg ccgcgcggac cagccatggt tcagccgcag caccggcgcc agcaggatga
     1741 acaacaccga aaacagcagc aggaacagcg tattgtaata gtcgttccat gccgaggcgg
     1801 cgaggcagag gccggcggcg acgaaatggc gcggccggtt ctcccgcgcg ccgcgcagca
     1861 gaaaataaag aaaccacggg atgagttccg tgctgagaat gttgagatgg ccggtcgccc
     1921 gcaccatgcg aaagctgctg aaggcataga tcacgccggc caggagcgca cccggcgtgt
     1981 cgccggcgac ccgacgcgcc agcgcgtacg tccccaggcc ggcaaccacg aacgacccca
     2041 tcaggaaaag attggaccag aaatacgggt tctcatacac gaacgcgccc agcgtgtaga
     2101 gcaggccata gaagaacgag tgcgtgtaat gaagcatcga gatgtcgaac gggcggtaaa
     2161 agtaatcgac gaaaaacggc aactggtgat cggcgagtag cgcgcgccgc gtccaactga
     2221 aattccaggc ccacaacatc gaatcgcccg gcgtgcccag cagcaggcga tgcggccgcg
     2281 tgacgagcgg ccatgtcgcg acggcggtca gcatcatgta gagcagcagg gcgagcagat
     2341 gggccggcgc atgcgaacgc cgccgccgaa gcgactccag cagcgcgccg gccccgcgac
     2401 gcggtcgcgc gccgccctct cccggagggc gcgcgacggc gggcgggtgc gtcggagaat
     2461 gctccacgat cctcggaacc tcggcgggag tcgcgttcag tcggttggca gtgtatggaa
     2521 gcgccctgcc cggcgcaaga gtgagcaaca cggtccagtc ggctttccgg cccgccgatt
     2581 gcattgattc ttggcggatg gcaaacaggc gggtatcatc gggttcatgc tgtcgcggcg
     2641 ccctgctccg cggcgtcccc caccatccga tcgcgaattg aggcccggcg ttgggatcga
     2701 agccatggac gacgttcctc attctgcggg cgatgcgccc ccgccagtgg atgaagaatt
     2761 tgctgctgtt cgccgggctg ttcttctcgc gcaatctcct cgacccgctt tcggtcaagc
     2821 gcgcgctggc cggcttcgtg atcttctgcg gcctgtccgg cgcggtctat ctgctcaatg
     2881 atctgatcga cgcgccgcgc gaccggctcc acccgcgtaa acggcatcgg ccgatcgccg
     2941 cgggtcagct cgctccgcgc gacgcggtcc tcgcgatcat cctgctcctg gcggccggcc
     3001 tcggcgccgc cctctggctc tcgtcgtact tctggatctg ctgcgtcgcc tatctggtcc
     3061 tgatgctctc ctacacgacg attctcaagg agatcttcct gatcgacacg ctgatcatcg
     3121 cgctgggctt cattatccgc gcggtttcgg gcgtgatcgt gctgcgcacc cccgacaacc
     3181 aggtcgacct gacgtcgtgg ttcgtgatct gcgtcctgtt cctctcgctg ctgctggcgt
     3241 tctgcaagcg gcgcagcgaa ctggtgaacc ttgagggcag cgcaatcgag ttccggccgg
     3301 tcctctcgct ctactcgctg cggatgatgg acagcgtcat cgccgtctgc gcggccggct
     3361 gcatcctctc ctacacgctc tacgcgacgc acatggagga actctggctg atgctctcga
     3421 cgctcccctt cgtcctctac gggatttttc gctacctcta cctggtctac aacagcgacg
     3481 taacggaggc gcccgaacat gccctggcca gcgacccgcc gctgctggga tgtgtgttcc
     3541 tctggggctg ctcgctcgtg ctggtctact ttcccgcctg atgcgcgccg gggcgccgcg
     3601 caccgtgccg tcgcgggcac tgctgctggg cgcgctctgc ctcttgttct atcccgatgc
     3661 ggccgcgcaa ccgccggccg aatcgctcct gcgggagcgc ccggcgccgg cgtcgtatcc
     3721 cgatcccatc gtgccgtggc tggggctgcc gtccgatgcg ccgccgatcc tccactaccg
     3781 ccacgatatc cgcatcggcc ctcccggcgc gggcctgtcc atccgcgaaa gccgttggca
     3841 ggccgaccgg ctcaagttgc tcggctttgg cggcagcctc gggctggtcg gcgtcggaac
     3901 cgtttcggcc gaagcaccgc tcgatctggc gacgacggta acgctcgaaa cgatcgacct
     3961 gaacgcggtt ctgcgctacc tgcaactgcc gcgcgccgag gaaatcgaag gccgagtttc
     4021 cggaaccgtt catctctcgc tgctgggccg cgaatggcgc cgcatcgacg tggccctcca
     4081 cgctcccgcc ggaaccgtcc ggatccaccg gcggctgatc gccgacgtgc tcaccaccta
     4141 tctgagcgag cagctcagcc gcgaggagat ctatgcctcg ctggaccagg cctaccgcgg
     4201 catcgagatg attcccttcg acgcggtgcg cgtcaacggc cgcttcgacc agcagacgtt
     4261 gaacctgcgc gtcccgctgc gcaacgagat cctgaatctg gacctcgagc cgcgcatcga
     4321 gcgcgcgatc ctttgggacc tgtgggactt gctgcgtcgg acgggctttg aggaacttga
     4381 ggacatcgag tggcggaccc gtcgttagta tgacgctgta aaggttcatc ccgcgccaca
     4441 gccccccgtc cccccccagg cctccggggt gctccccgtt ttgaattgcc aaccctttcc
     4501 accgccatca tcatgggcgg gaatgctgga gtccgacata aacgtgcggc gtgtcatttc
     4561 cgtcccctgc atccctgccc tgacccgcga acaaccgaac caccactccc cgtgtgaaag
     4621 ccgatgaccg tcccccccaa acacaagccg ttcggcctgc gccatggcgt gtggctcgcg
     4681 ctgctggcgg ccggctgtgt cggcttcacc gtcgcgcagt ggtcgcgcgg ttatgtcgat
     4741 ttcggcgacg gcaattacat gtacatcggc atgcggatcg ccgaaggcgt cgtcccttat
     4801 cgcgacatcc tggcgccgca gcccccctgc cacctgttca tgggcgcgct gatctacaaa
     4861 ctgtcgcagg cgttccgtcc cgaaaccccc gtctacttct tccgcggcta ctcggttgcg
     4921 ctgcggctgc ttaccctgct gctggtggtg gcgctggcga tgcgcgcctg gggccgaccc
     4981 tcgacggcgc tggtggcggg ggcgatctat ctctggtttc ccatcggctt ctggtggtcg
     5041 tttgcctttc aatccgaacc gctcgagatc gtctttctgc tggccatggc atggtgcgcg
     5101 ctgcggggga cacgcgccgg cgacatcggg accggcatct tcgccgccct ggccgccctc
     5161 accaacgcga ccgccgcccc ttttctgctc gtcctgatca tcttcatgct gttgcacgcc
     5221 ccggggcgcg cgctgcgcat ggcggccccc tgtctgctgc tggcgggcct cgtcacgctc
     5281 gcgctgcaca tctggaccga aggcgccttc ctggatgtcg tcgtgttcaa gcaggtggga
     5341 acctaccccg gcgggctcgg ttcgccccag ttcctgaact acgcgcgcgg caaactgctc
     5401 gaccagggcg gcaaggtcct cgcgctggag ggcgcattct gggtcatcgc gttgatcgcc
     5461 gttccgcgct atttccgctc cagtccgctc ccgccgatgg cgcgcgccgg actgacctgg
     5521 ttcctgctgg cgaccatgac ggcaatcctc tacgtcacca agggcggcac cgccgactac
     5581 atcttcagcc tcagcgagcc ggccgtcgcg atcctcgccg cgggcgaact ggtcgcctgg
     5641 gcgcggcgct ggaaaacccg cgccgccccc gaaatcggcg gcggcttgtc gatccgctcg
     5701 atcccgtttg ccctccccaa actggcggcg gcgtggatgc tcgcggtcct cgccctcggc
     5761 ccgggcctca atttccacgc gctcctgtgg gatcaacgcg cctacgagct gagcgaacgc
     5821 ggaacccccg accggcccgg cgtccgcctc gtgcggcgga tcatccagga gcacagccgg
     5881 ccgcgcgatt tcattctggc gccgccgtat tacgccgtcg attccgaccg gcgtctgtgg
     5941 ggcgagttca gcgagctgtt catctggaac atcacctacc tgaacgacct gcgcgccggc
     6001 ttgcgcgagg gaaccggctg gcgcaagatg cgcgagatcg gcgaagcgct ccgcgcccag
     6061 cagattccaa tcgtcatcct cgagatgggc cagaccggca tgtcccgtga ggtgatgctg
     6121 gcgctccagg atcgctacgt cccgctacgc ccggatcggc gccggttcct gctccacacg
     6181 ctcaacacac ggttgggctt ctttatcccg gcgccgccgc tcgaagacga aactgccgcc
     6241 gccgaacgga tccgtcagtg ggaactgttc aaactcgagc tgctcgaagt cttcgggccc
     6301 ggcgcggcga acgatcaatt cgggtcctgg tacgccgagc gtccggccgg acccggcgcg
     6361 tcgagcggcg aggcgccgcc gcgggagcca gcgtgaacga gccgtacgat ccccattcgg
     6421 cctcgaccga cgaggaactg ctccccgtcg tcgatgagca cgaccgcccg atcggcgtgc
     6481 ggcgtcggcg cgagatccat cggctcggcc tgcgccaccg ctcggttcag gtctgccttg
     6541 tcgatccggc cgggcggatc tggttgcaga agcgcagccg ccgcaaggac accttccccg
     6601 gttggtggga catggcggta accgggcacg tcgagccggg cgaaacctac gccgaggcgg
     6661 cgatccgcga actgcgcgag gaactcggtc tggcggccca accgcgctgg atcggccgca
     6721 ccgccgccgc cgcgctgacc ggctgggagt tccaggaaca ctatctcctg cgctggaacg
     6781 ggccgatcac ggatttcaac cgcgaggaga tcgacgaact gcgccccttc cgctccgatg
     6841 agatcgaaca ggctgcccgc gcaaatcacc ccgatctgcg cctgacgccc ggcgtgatct
     6901 acatcctgcc ccacctgctg gcggccctga agccggagca aacgccatga accgcccgcg
     6961 ggtcgaaacc ggaaccgggc gggcggcgga cgacgcggcg gcgcggcgcg gcggcgcgca
     7021 cgaaccgctc acgatccgtc gcgacagctt ctaccggctc gaatggctcg tccttctcac
     7081 ttgcctggcc gcggtgatcc tggtctatcc cctgcgcctg caggacgcct ggctcggcca
     7141 gctctaccgc tggtgggacc ggtccgatgg cttctggtcg cgctggacgc acgcgcagtt
     7201 cggcgcctac ctttcgttct tctactcgcc gctgatactg aaaggcgtca tcgccggcgt
     7261 cggggcattc atcttttgcg ggctggcgct ggcgcgcggg ctgctcggcc cgcacctccg
     7321 ctgcggaacg atcgtcgagc gtccacaatc gccgtgggcg tggtgttcgg tggcgctgct
     7381 gctcggctgg gtcctcgcct cgggcctctg gtcgcccacg ccggcacagg cgcgcgacgc
     7441 cgcctggttc gtcgcgccat tcggcctcct gctgtttctc ctcctgcggc gcggcctgct
     7501 gcgtcaggaa gcccgctggc ttgcaatcgc gctggtcagc ctgggcgcga tcgtgatggc
     7561 ggtcatgctg atgcaggcgc tgcccggcct cagcgagctg acctttcgcg tgatgacgcg
     7621 cttcgatgat ttccgcaatc tgtatggctc gctgatgggt cacaacaccg cggtcgcgtc
     7681 gctgctgctg atgacgctgt ttccggccct cgcgctggcc gcgggggcca ggaccaggcg
     7741 gcgccggctc gcgctcggcc tctatctggt cgcgctggtc tacggcctga tcgtcacgca
     7801 aagccgcgca atctggctca tcgggccgcc gctgatcgtc ggccagttca tcgcgctcct
     7861 gcgcgccacg cgcagtgagc ggcagcggcg ccggctgcgc gggctgccca cgctgctgct
     7921 ggcgatcgcg gcgctggcga tcctgaccca ggtgatccgc aagccgtgga atccgttctt
     7981 tgtccgcgcc tacacgctga gcgaacggct tcgcgacctg ggacccgatg cgctgcgccg
     8041 cgagtcgcgc ctgcggctca acgtcatcgg cgcgacgctc gtgcccgatc ggcccctcct
     8101 cggccacggc ctcgcggctt ttcagtatgt ctatcccagg cgccaggggg agtatttcag
     8161 ccggcatccc gagagccgcc tcggccgcac tgagaagcgc tcccacatgg cgcacaatga
     8221 atacctccag gtggcgatcg agcaggggct catcgggctg gccctgctaa tcaccgtcct
     8281 cgccgaagtg gcgcggcgcg gcgggcgccg cggccgccgg ctgcgcggcc gaagccgcct
     8341 gctgcacgag gccttcggct ggagcagcct cggcttcctc ctgcacgcgg ccgtcgattt
     8401 cccgtttcat atcccccaac tgctggttcc ggggctgctg tgcgtggcgg cgtggtccgg
     8461 cacgggcggc cttatccgcc gcgtcggcgg cgatgatccg gggcgcgcct tgccggcggg
     8521 ccgatccaat ttccgctggc cggccttcgc ccgcctgctg gccacgatgc tcctgttctt
     8581 cgccgtgccg ctggccggcc tgccgctcgt gcgccggctc caggccgacg tcgcctataa
     8641 tgaaggcggc gcctacctga cccgttatga cctgttcggc cagagttggc cgcccgagga
     8701 ggcgctggcg aatttcgagc tcgccgccca gtcgctccgc gaggccctcc gcctgcaacc
     8761 gagccattcc ttcgcctggc agaagctgtc gcaggcccag tggcttggcg gcctgatgaa
     8821 ggctcgcatg gcggccctgc gccaggccga cggcgcgccc gcggccgacc tgacccgcgc
     8881 cgcgctcgac gacatgaagg ccgccctcgg ctgcctcttc aaggccgccg aggggctcga
     8941 ttaccactat ttgccttatc agcgcggccg gatctatcaa tcgctctacc gcgtcaccgg
     9001 cgacccgcgc tatcagcagc tctacgtcga ggggctggaa caggcgctcg cctactccga
     9061 tgtgttcgtg gatggtttgc atgaattggc cgaggcgctc gcccggaatc cggcgacgcg
     9121 cccgcgcgcc atggagctgc ggcgccggat catccagttc tggcccgatc atttcgtccg
     9181 ctggtacgtc gacccggcct acgagctgat tgcgaacaaa cgttaccagg cggcggcccg
     9241 cgaactgggc tggatcgtcg atctgtgggc ggaccgcgat ccctgggtcc tcatcgaagc
     9301 cgccctggcc catctccgcg ccggcgaccg cgaccgggcg ctcgaactga tccgccaact
     9361 tcgggagcgc gatctgccga cctatcatgg cgccggcggc catctgcttg agccccttct
     9421 gctcgggcaa tgggcgcagg cttacgaagc gctggcgagc atcatgccgc ccgacgcccc
     9481 ctcgcgcgcc gaaatgaagg tcgtcgaaca cgaactgcaa cgacgcctcg gccgcaccac
     9541 cgagccctcg cgctacccac cgccagaagg cgtggcggcc gacgcatggc aacggctggt
     9601 cgccgagacg cgcccgatgg tcctgctgca cctgttcaac gatcctgagc gggccgaaca
     9661 ggcgctgcgg gaccgactgg cgctgcccgg gccgccgccg gcggtcgatt tctggctcga
     9721 agcgatctac atcgcgcgcg cccggggcaa cgccgagctg ggacgcgaag cgctggaacg
     9781 ggccgccgcg ctcgatcccc gccacccggc gctcctcgcc ctgcgctcga cgcccgacga
     9841 ggaaccgtag cgcctgtccc ccaatcccta tccgcttgct ttgactgatt ccgctcttta
     9901 cagtgttcgt gaccgctctt aaccgccccg ccgggcgccg cggatggccg ccgacgcacg
     9961 ggcgcatgac ccctccccga tgggctagca ggtcggattg attgtgaatc gcgtcaagcc
    10021 cccgacgacc gaagcgtcgc aaacccgcat cctgatcgtt gatgatgaac cgatgctccg
    10081 ctcggtcgtc caggacttcc tggccctact cggctatcaa aagtactggc tggccggcga
    10141 cgggcgcgag gcgctcgaaa tcctccgcgc ccaccccatc gacgtcatgc tgagcgacat
    10201 ccgcatgccc gaaatggaac tcgaggagtt gctgacgatc gtcaacgacg aattccccgg
    10261 tctggtcgta atcgccacat cgggttattc ggatttcgaa agcgcgcgca atatcctctg
    10321 ccgcggcgcc cacgacttcc ttgaaaaacc gctcaacctg gacgcgctgg agcaatccat
    10381 cggctgggtc atccgccgcc gcagaatcct cgacgaggtc gctgaacaat tcggcgccgg
    10441 cccgactggc gacgaagcct ggcgccggct cgaccacctg tgcgacacgc tgaccgacgc
    10501 cggccgcacc ttcccgcgcg ccctgctcca ttgcgtccgg acctatcagc tctcgcgccg
    10561 gctcgacctg ccgctcagcg aagaggaaca tcaggaactg gccctggcga tcctcctgca
    10621 cgagatcggc gccagcctgc aggttcattc catctgcgcc ctgccgcgcc tgctcgacga
    10681 ggaagaactc gccctcgtcc atcatcaaag ccgccacagc gcccgtctgg tcggccaggt
    10741 gctcgatcgg ccgccgctcc tggcgatgat cgccgaccac gtccactggc gcgagatcag
    10801 cgccctggat ccgaacgcaa acgcccagac ccgcctgacc gtctggctcg gactgctcaa
    10861 tctgctcgac ggctgcctca acagccgcgc cgaccgcggc ccgattccca tcgatcagct
    10921 ccgccattcg ctccagcgcc gcgcccgcca gtccccgctg gccgccgtcg atcatctgct
    10981 cgccaagtgg cccgtcgtgg aacgcttcta cgcatcatca ctgtagcaag cgtcccttcc
    11041 tgcttcgccc ccgatccccg tccctcgtcc gcggctctcg aaaactctcc atctgagcaa
    11101 ttcagaccga cgctcacgcg gcaaattccc agccacggaa gaggccgatc cccacccgca
    11161 tccgaaccat ctcaaatcct tacggtcttt gagttctcta cggctgggcg gacggcgttt
    11221 cgccctcgga cgcgcgctga aacgtcagcg acgccgagtt caggcaataa cgcaaccccg
    11281 tcggcgccgg accgtcctcg aagacgtgtc ccaggtgcgc gtcgcacttc ccgcagacca
    11341 cttccgtgcg ccgcatcccc aggctgtcgt ccggcagctc cttgacccgc tcaccgctga
    11401 tcggctggaa gaagctgggc cagcccgtcc ccgaatcgaa cttcgcctcg gaagtgaaca
    11461 acggctcgcc gcagcagacg caatgatacg tcccggcttc cttgtgatgc acatactcgc
    11521 ccgtgaaggg ccgttcggtc cccttctcgc gcgtgacgta atactgctcc ggggtcaacg
    11581 cctgcttcca ttcttcattg gttctcacgt cgcatccctc catcggtaaa gacgccgccc
    11641 gcggctgcgc ggcggatagt tgcggctcgg gcggcggggg ctgcctccgg catccgggga
    11701 tggccgccgt cccgcccagc gccagcatga tcacgatgcg ccagatcgtc gccatgtgcg
    11761 tttctcctgc tctgcaagat gccttttggt cggctcgagc ggcgaaccct gacagccgcg
    11821 gcgacgattt cccggctgcg ttcgccgggt tgccccctaa cttcggctcg tgttcggcgc
    11881 ggacacgtcg aaattggcca acgccgacag ccgcttgacg acttcctcga tctgctgatc
    11941 cagttgcgac tcctcttcgt gcagccgcgt cactcgcgcc gtggcctccg gcagcagttc
    12001 atgatggatc tcgttcttgc gccggcgcaa ctccttcagt tgctcctcca gcaacgccat
    12061 ctcttcgttg agcagcgcga tgatgcgctg gcggatctcc agcagcgatt gtttcaggtc
    12121 gctctcggcc cggttcagcg tcgattgcat ccgctcgatc tgccggtcgg tctcttccag
    12181 cttccgtcgc gtgtcgacgc tgccgttgtc gctcgccacc aggttgtaga attcctgccg
    12241 gcggctctcc agccagctca accgctgccg cagccggctc gtctcggttt cctgcgtctc
    12301 ctggatcgcc cgcatctcgg taatcagtcc ctgcaggccg gtgccctggg gacgcgtggg
    12361 ctggcttccc gacgatgata ctccactgct catgcgttca gttgttctcc gtttttcgga
    12421 cccgccatct cactcccccg ccagggcgca tttcgcccgc cggacggcaa tgacgaaaac
    12481 cgcctgttcc tccgcgcgca cgcccatgat cggcggatgg cccttcggga tgggtgatgt
    12541 cggagagcag tccacttcga accgctcccg tatgtgcctc tgccgtggcg ctcagattat
    12601 atcctgaatt caacccttcg ccaatcccat tccccttcca taaacttcgt ctctcgtcct
    12661 cgtccttatg atcgccccca tgggcccgcc ccaattctta ctgcttcgtt gtgaaaccgc
    12721 cggttcatgg aactcgtagc cgcccccgaa ggccggcccc gaattcggct cccggctctc
    12781 gtccctcgtc ttcggctctc gaaatcctgc cctttcgccc cctcgtcctc cctcctctct
    12841 ctccgaccac caatccattt cgtgtcaatt cgtggctttc tagatccaac tccattcatt
    12901 tttccgtttc ttcgtgacgc accacatggc gcatctcaat cgctttctca aaattc
//
LOCUS       DAOPCO010000089        12920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4813_length_12920_cov_4.700194, whole genome shotgun sequence.
ACCESSION   DAOPCO010000089 DAOPCO010000000
VERSION     DAOPCO010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_12920_cov_4.700194"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLS90_07990"
     CDS             <1..418
                     /locus_tag="PLS90_07990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02385.1"
                     /translation="RRVYLALGGLDPGLPYPGEDADFCWRVADAGYRLSYAPEARVTH
                     HHRATLRALWIQSCHWGIGQADLFVKWRSRWRAWCWFKPVLLVWAAKGLLKSPWRWLR
                     GRTPFERREPWLDFLANSAMLWGRFWGGLRRGTLVF"
     gene            591..2282
                     /locus_tag="PLS90_07995"
     CDS             591..2282
                     /locus_tag="PLS90_07995"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HPK02386.1"
                     /translation="MADQTDQATLSKVEFIKENSDGLRGTIEEVLNSDASHFEENDLQ
                     LLKFHGSYQQDDRDLRAERRKAKLDKAWIFMVRAKIPGGRVTAEQFLDMLDLGCKVGN
                     GSIRITSRQGIQFHGVGKENLRYTIRRINQTALSTLGACGDVNRNVMCCGLVDQDWRR
                     SLGMEQLCLQVADHFTPHATGYWEIWCDGERFGQKVEHTTHEPIYGARYMPRKFKIAV
                     TVPEDNCVDVYTNDLGVEVVHHNGRVMAYDLIVGGGMGFAHGNEATYPRLGTRMVRVT
                     PAELLEALETVLKIQRDYGDRSDRKHARLKYVVEERGAEWFRDEMRRRTGRNYPEAGP
                     LPPYKVLDHLGWEQRFDGRWNVGLHIDDGRVRDRDGAPLMTGLRRIVERFRPQVRMTP
                     RQDVVLVGIDENDRPEIQRLLDEHGLETDAGLKRMRRLSMACVALPTCGLALAESERY
                     MPSLLRQLDALGVGDEPVEIRMTGCPNSCVRSPLAEIGVVGRGPGKYALYLGGNQEGT
                     RLAFPFLERVDDTQLAQVIFRLIDAWRRETGRAASFGDWSTQKGCQALEPIAALD"
     gene            2296..3828
                     /locus_tag="PLS90_08000"
     CDS             2296..3828
                     /locus_tag="PLS90_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HPK02387.1"
                     /translation="MSPFQFPDVPYTIEKIMESIRVQIVEGAAETEPESAHPPNELTP
                     TAPRPLVDTGDPLSQLAAQVDLLRQHQGLDPGYRVRSHRLVLGPLINLVKRVIHWGSR
                     PYTDAIRRRQEAFNDVAVQALREVMIELERARRQIADTRAELHADLRRENAALRQDLQ
                     SLQTLIHQVQEEAGRKLFQELEDLGGRLTQLIHDSIQQATRYQEWLVEEKQHYLEGMI
                     ADVARKAAEEAEQIRRNHAARLDELARSARALIARHDLGPFFSRVPEDKRLATMATTR
                     GTWEDISGRQAFYVDLLERAPGQVLDVGCGRGELLAQLLHRGVECWGAEIDPLLVRLC
                     RERGLHVVQCDALAALRGAPPQSLGAVFAAQVVEHLFPGELLCFLRLARTRLARGGLL
                     VLETLNPAALAVLAKSYYRDIDHKQPLHPEYMKLLAELAGFEPVELHYLAPFHETERL
                     PDLPPAESLGLSGAARQSLQERFDQLNRILYGMQDYYIVAHQGEPAPVDSRDDDGGAA
                     NT"
     gene            3825..6140
                     /locus_tag="PLS90_08005"
     CDS             3825..6140
                     /locus_tag="PLS90_08005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02388.1"
                     /translation="MKLALISSSPVPRVYGGMDRLLEGLTEALRQRHPTDLITYPVDE
                     RTADGVLRGYYDFYTMDLSAYDAVISYKAPAYMVRHPVQVLYLSHRLRVFYDLYEQRD
                     AHHAKMRRLVQWMDNWAMSPERIPHIFCVGKTVTRRLRRWGGIASTPIHHPTTFVPDP
                     PREGEHFLAVGRLHPWKRFDLIIQAMRASRCDVPLLIVGSGPDEGRLRELAAGDSRIR
                     FLGHVDENRLRELYARSIGHIFPPINEDLGMITFESFLAGKPVLTTTDSGEPAEIVEH
                     GKTGFVVEPNPAAMAERLDWMATHFSELEEMGRHCIERVRDVTWERLVDRLLDAVEKT
                     RQARAGSQVAIHTDRESPRPRDEKIHLLVTDNQIIDPPQGGGRVRIWQLYRHLPADFV
                     TTYLGTHDHPGPVFRDQWLAPNFREIIMPLTIRHFKMHEVWRRLTRGDATVDVTIPLL
                     LGRHSPRYQRLLHEHLQRADLLIVSHPWMTPWLPSDGPPLIYDSHNCEPAVKEPLLKR
                     TLAGRYLARRVEQVEREAVTRARLVLACSHADAEQFERRYGAPPEKLLHIPNGVDCDA
                     IAPVEATRRHILRRRLGIPDRPFAIFVGSHYGPNMDAAEFLIRQLAPQLTELTVGIIG
                     GVGPAWREQHGDELPANVRLFGFVDAEQLVQLYQCADLGLNPMQQGSGTNIKMLDYMA
                     AGLPIVTTETGARGLAGENGVHWCNATVFEFPQAVRELLAAPARAAEMGRAARRLAEE
                     VYDWARISLRLLLGRDAAGAELVATLREDSA"
     gene            6137..7186
                     /locus_tag="PLS90_08010"
     CDS             6137..7186
                     /locus_tag="PLS90_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02389.1"
                     /translation="MKLLVVSTYPPLACGIGRYAAQQVDFLRREGHWVDVLTPAGCDG
                     DFQDDLYGRFRILALLKYLWAYDMAYLHFAPQFFYDTASRASRFMTSFGFLLVMLLAG
                     KRITFMIHETGYKIGQEGRGLLRHRIDRWYWRLAGRVVFHSTRERDTFCWFYRLKTTR
                     ANLEVWPHDKYMVPLWPHDRAEARRRLSLEPDKLFFLCIGFVQPHKGFDRALEAFLKT
                     PGEHLRFKIVGSVRINWDVAHQYAQRLHELAEQDARAEFVESFVNDEMFDMWVAAADY
                     VVLPYREIWTSSVAARARLYGRPLIAADTGGLAEQLTEGSMLFRDDDELVECLRRIAA
                     RTNPSPAGEPTPTPH"
     gene            complement(7281..10049)
                     /locus_tag="PLS90_08015"
     CDS             complement(7281..10049)
                     /locus_tag="PLS90_08015"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HPK02390.1"
                     /translation="MKLKTHLRECNLDQLRAIADWWGLQLEAGEPALDAAGLAEYLYP
                     RMQVASNFRNAFDQLTPAQRDWVYFLAIHGGELPIEEFRKRCRFETADAMNEALAPLF
                     ERGFLWRERLRDKLISHDLIGIPEPFVRLVDLPPYWQGFLGYYLQALGTDDLRAITKH
                     TLETSAPTRRKQALVHFLRKRLLDPKSLQQVLARLNPLQVEMFKQILQKNGVCAWKDL
                     LDAGVHKKFDHQRADKLRELVEQSGLVFVLRSAPNKYNNLLLVPRDVAWVIQNGYKRD
                     ERSLEELSHSGPEATTRKGAEGGMRPGVILDNSNNILRDLVILCAYIQRNPVKMLNNG
                     SLGRNDLKKIVPLMSHNKTIKYVSFLALFAIVRKLIIAVGDQWRVSSSLGRWLEGGQA
                     CYRDLYQFWLNTSEWNEEFIDGDVVHADNYPQNLISIIELRKLVLRMLEKLPSETWID
                     FETFAESLLPQIAIEIPGRFDLTPADKHNRHPTLIMESVVAETLYWLGLVVLGVSDLE
                     VARQLGSRPNEAIAPYDPSRPFSSRLVGDEQLMFMFRLSETGAEILTRTYLDPEKLFA
                     RMPDGALPFASQSTYFTVQPNQEIVTPPDLQLDKFFTLLQFADIKKVDVMTTLTISHD
                     SIRAGLDSGLTARQMLALLQECSRKDVPETVRQMISECESRHGELDMGMAGGYLRVAD
                     RHHLEELRANSKIAPYIKDVFDDRLVLLSRTTDFRKLTRELQRLGYLPRVDSDSLYVT
                     NEGLFQITLREEELYDLLAILQFGILLEEEKEASIFEDRIQPLFQRLSGHAQENFNPK
                     FYAESIARNFIANYEKANKKTIDEETRKYRKQLNRLLTQMPKARALSGYKGENPAREP
                     AEIGKLVRYAIEHEKPVRILYERTTGEQHELTIEPEALQGKKVYAYCPDQDEHHIYSL
                     ERVQQASI"
     gene            complement(10098..11828)
                     /locus_tag="PLS90_08020"
     CDS             complement(10098..11828)
                     /locus_tag="PLS90_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HPK02391.1"
                     /translation="MSQEKPLIVQSDRTMLLETDHPLYEAARDVLAAFAEIVTSPEHF
                     HTYRITPLSLWNAASAGLTAEEILASLRAYSRYAIPQNIEYDIREYVSRYGRLRLVQQ
                     GDALVLECDDALLLEQFYADERIRRYFEKRLTRTSAQVKPNERGFLKQALIKKEFPVE
                     DLAGYEEGERLEVALRERTAAGEPFAPREYQREAITVFHQYGRASGGAGVLVLPCGAG
                     KTIIGLGILQAIGQQTLIVVTNITALRQWRDEILDKTTLTPDEIGEYSGEVKEIRPVT
                     ITTYQILTYRKRREEEFVHMELFNRRAWGLIIYDEVHLLPAPVFRAVAGIQGKRRLGL
                     TATLLREDGKESDVFSLIGPKKIDIPWKTLERQGWIAQALCTEVRIPLPEHVRHEYAV
                     ADAKAKFRIASENDEKIQVAREIIRRHPDDQVLIIGQYLDQLEKVAAEFSAPIITGKT
                     PNREREQIYADFRNGVIHVLIVSKVANFSIDLPDAAVAIQISGTFGSRQEEAQRLGRI
                     LRPKANGNMAHFYTLVTQDTRERDFAVKRQLFLTEQGYRYRIVSSLDELEPEEDAATD
                     HADLGRAAMN"
     gene            12177..>12920
                     /locus_tag="PLS90_08025"
     CDS             12177..>12920
                     /locus_tag="PLS90_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02392.1"
                     /translation="MHAVRNLLVNLVRRDLTLRYRSTVLGFFWSFVKPLALTAIFYIV
                     FTYVLRIPLREARVPFVLHLLTGMLAWTFFAGATGEAMDVILANANLIKKVRLPLAVF
                     PLATVCSHLVHFFLALLVLVGLLIVADLPPTPAILLVLPIAALQFLLVLAVAFVLSAL
                     NVFYRDIASVWEVLTTAWFYATPIIYPVYMALEEIDRRGWGWLRLPYLANPMTPLVLA
                     YRRVLLYAGVGDTPQAPRLLEIPDFALLAG"
BASE COUNT         2144 a         4610 c         3835 g         2331 t
ORIGIN      
        1 ccgccgcgtc tatctggccc tcggcgggct cgaccccggc ctcccctacc cgggcgagga
       61 cgccgacttc tgctggcgtg tcgccgatgc cggctatcgg ctgagttacg cccccgaggc
      121 gcgcgtaacg catcatcacc gcgccacgct gcgcgcgctc tggatccagt cgtgccattg
      181 gggcatcggc caggccgatc tgttcgtcaa gtggcgctcg cgctggcgcg cctggtgctg
      241 gttcaaaccg gtgctgctgg tctgggccgc caagggcttg ctcaaatccc cgtggcgttg
      301 gctgcgcggc cggacgccct tcgagcggcg cgagccgtgg ctcgattttc tggccaattc
      361 ggcgatgctc tgggggcgct tctggggggg cttgcgccgc ggcacgctcg tcttttgacc
      421 cgcgcgcccg cttgcaaagg ctgggccttt cccctaaaat gtggcctctg acgggtctgg
      481 acacgggggg cggagacgaa atgcctgtca gccactgatg ctcgccgcga ccggccgggc
      541 gcaacaccga cgcatccctt gcacgctgaa tttcggaaga aggacgtaac atggcagacc
      601 aaaccgatca ggccactttg agcaaagtgg agttcatcaa ggaaaacagc gatggcctgc
      661 gcgggacgat tgaggaggtc ctcaacagcg acgccagtca ttttgaagag aacgacctgc
      721 aactgctcaa gttccacggc tcctatcagc aggacgaccg cgacctgcgc gccgaacgcc
      781 gcaaggccaa gctggacaag gcgtggatct tcatggtccg cgccaaaatc ccgggcgggc
      841 gcgtcaccgc cgagcagttc ctcgatatgc tcgacctggg ctgcaaggtc ggcaacggct
      901 cgatccgcat cacctcgcgc cagggcattc agttccacgg cgtcggcaag gaaaacctgc
      961 gctacaccat ccgccgcatc aaccagacgg cgttgagcac gctgggcgcc tgcggcgacg
     1021 tcaaccgcaa cgtcatgtgc tgcggcctgg tcgaccaaga ctggcgccgc tcgctcggca
     1081 tggagcaact ctgcctgcag gtcgccgatc acttcacccc ccacgccacc ggctactggg
     1141 aaatctggtg cgacggcgaa cgcttcggcc agaaggtcga acacacgacc cacgagccga
     1201 tctatggcgc gcgctacatg ccgcgcaagt tcaagatcgc cgtcaccgtg cccgaggaca
     1261 actgcgtcga tgtctacacc aatgacctcg gcgttgaggt cgtccaccat aatggccggg
     1321 tcatggccta cgatctgatc gtcggcggcg ggatgggctt cgcccatggc aacgaggcga
     1381 cctatccgcg gctcggcacc cgcatggtgc gggtcacgcc ggccgaactg ctcgaagcgc
     1441 tcgaaacggt cttgaagatc cagcgtgact atggcgaccg cagcgaccgc aagcatgccc
     1501 gcctcaagta cgtcgtcgag gagcgcggcg ccgagtggtt ccgcgacgaa atgcgccggc
     1561 gcaccggccg caactacccc gaggccggcc ccctgcctcc gtacaaggtg ctcgatcacc
     1621 tgggctggga gcagcgcttc gacggccgct ggaacgtcgg ccttcacatc gacgacggcc
     1681 gcgtgcgcga ccgcgacggc gcgccgctca tgaccggact gcggcgcatc gtcgagcgct
     1741 tccggcccca ggtccgcatg accccccgcc aggacgtcgt cctggtcggc atcgacgaga
     1801 atgaccggcc cgagatccaa cggctcctcg acgagcacgg cctggagacc gacgccgggc
     1861 tgaagcggat gcgccggctc tcgatggcct gcgtcgcgct gcccacctgc ggcctggcac
     1921 tggcggaatc cgaacgctac atgccgtcgc tcctgcgcca gctcgatgcg ctcggcgtgg
     1981 gcgacgaacc ggtggaaatc cggatgaccg gctgccccaa ctcctgcgtc cgcagcccgc
     2041 tggccgagat cggcgtcgtc ggccgcggtc ccggcaagta cgccctctat ctgggcggca
     2101 accaggaagg gacccgcctg gcctttccct tcctggagcg ggtggacgat acgcagttgg
     2161 cgcaggtcat cttccgcctg atcgatgcct ggcgccgcga gaccggccgc gccgcgtcgt
     2221 tcggcgattg gagcacacag aagggatgcc aggcgctcga gccgattgcc gcgctcgact
     2281 gaacggcgcg ttcccatgag cccgtttcaa ttccccgacg tcccctacac gatcgaaaaa
     2341 atcatggagt cgatccgtgt gcagatcgtc gagggcgccg cggaaacaga acccgaaagc
     2401 gcacaccccc ccaacgaact gacccccacg gcgccgcggc cactggtcga caccggcgac
     2461 ccgctgtccc aactggcggc gcaggtcgat ctcctgcgcc agcatcaggg gctcgatccc
     2521 ggctaccgcg tccgctcgca ccgcctcgtg ctggggccgc tgatcaacct cgtcaagcgc
     2581 gtcatccact ggggttcgcg gccctacacc gatgcaatcc ggcgccgcca ggaagccttc
     2641 aacgacgtcg ccgtccaggc cctgcgcgaa gtgatgatcg aactggagcg cgcgcgccgg
     2701 cagatcgccg acacgcgcgc cgagctccac gccgacctgc ggcgggagaa cgctgccctg
     2761 cgccaggacc tccagtcgct ccagaccttg atccaccagg ttcaggagga ggcgggacgc
     2821 aagctgtttc aggagctgga ggacctgggc ggacggctca cccaactcat ccacgattcg
     2881 attcagcagg ccacccgcta ccaggagtgg ctggttgagg aaaagcagca ttacctggag
     2941 gggatgattg ccgacgttgc gcgcaaggcg gccgaggaag ccgaacagat ccgccgcaac
     3001 cacgcggcgc gcctggatga actcgcccga tcggcccggg ccctgatcgc ccgccacgac
     3061 cttgggccgt ttttcagccg cgttcccgag gacaaacggc tcgccaccat ggcgacgacc
     3121 cgcggcacgt gggaggacat cagcggccgc caggcgtttt atgtcgatct gctggagcgc
     3181 gcccccggcc aggtgctgga tgtcggctgc ggccgcggcg agctgctcgc ccagctcctc
     3241 catcgcggcg tggagtgctg gggcgcggag attgatccgc tgctcgtccg gctgtgccgc
     3301 gagcgcgggc tgcacgtcgt ccagtgcgat gccctcgccg cgctgcgcgg cgcccccccg
     3361 cagtcgctcg gagcggtctt cgcggcgcag gtcgtcgagc acctgttccc cggcgagctg
     3421 ctctgcttcc tgcgcctcgc ccgcacccgg ctggcccggg gcggcctgct cgtcctcgag
     3481 acgctcaatc ccgccgccct tgccgtgctg gccaagtcgt actaccgtga tattgaccac
     3541 aagcaaccgt tgcatccaga atacatgaag ctgctggccg aactggccgg ttttgagccg
     3601 gtggaattgc actatctggc gccgttccac gagaccgagc gcctgcccga cctccccccg
     3661 gccgaatcgc tcggcctcag cggcgcggcg cgccaatccc tgcaggagcg cttcgaccag
     3721 ttgaaccgga ttctctacgg gatgcaggat tactacatcg tggcgcatca aggggagccg
     3781 gcgccggtcg actcgcgcga cgacgacggc ggcgccgcca atacatgaag cttgcgctga
     3841 tcagttcctc gcccgtgccg cgcgtctatg gcggcatgga tcgcctgctg gaggggctta
     3901 ccgaggccct gcgccagcgc caccccaccg acctgatcac ctaccccgtc gacgaacgga
     3961 cggccgacgg cgtcctgcgc ggctactacg atttctacac catggacctg agcgcctacg
     4021 atgcggtgat cagctacaag gccccggcct acatggtgcg ccaccccgtc caggtgcttt
     4081 acctgagcca ccgcctgcgg gtcttctacg acctctacga gcagcgcgac gcccaccacg
     4141 ccaagatgcg ccggctggtc caatggatgg acaactgggc gatgtcgccc gaacggatcc
     4201 cgcacatctt ttgcgtcggc aagacggtca cgcgccgcct gcgccgctgg ggcgggatcg
     4261 cctccactcc catccatcat cccacgacgt tcgtcccgga tccgccgcgc gagggcgagc
     4321 acttcctcgc cgtcggccgc ctgcatccct ggaaacggtt cgacctgatc atccaggcga
     4381 tgcgcgcgag ccggtgcgat gtccccctgc tgattgtcgg cagtggcccc gatgagggcc
     4441 ggctgcgtga actggccgcc ggcgacagcc ggatccgctt ccttggccat gtcgacgaaa
     4501 accgcctgcg cgagctttat gcccgctcga tcggccacat ctttcccccg atcaatgaag
     4561 acctcggcat gatcaccttc gaatcgttcc tggcgggcaa gcccgtgctg accaccaccg
     4621 attcgggcga gccggccgag atcgtcgagc acggcaaaac cggctttgtc gtcgagccga
     4681 atcccgccgc catggccgaa cggctcgact ggatggcgac ccatttcagc gaactggagg
     4741 agatgggccg ccactgcatc gaacgcgtgc gcgacgtcac ctgggagcgc cttgtcgacc
     4801 gcctgctcga tgccgtcgag aagacgcgcc aggcgcgcgc cggcagccag gtcgccattc
     4861 ataccgaccg cgaatcgccc cgcccccgcg acgaaaaaat ccacctgctc gtcaccgaca
     4921 atcagatcat cgacccgccc caggggggcg gccgcgtccg catctggcag ctctatcgcc
     4981 atctccccgc cgacttcgtc accacctacc tcggcaccca cgatcatccg ggaccggtct
     5041 tccgcgatca gtggctcgcc cccaacttcc gcgagatcat catgccgctg acgatccgcc
     5101 acttcaaaat gcatgaggtc tggcgccggc tgacccgcgg cgatgccacc gtcgacgtca
     5161 ccatcccgct gctgctgggc cgccactcgc cccgttatca gcggctgctg cacgagcacc
     5221 tccagcgcgc cgacctgctg atcgtctccc atccctggat gaccccctgg ctgccttccg
     5281 acggtccgcc gctgatctac gactcgcaca attgcgaacc ggccgtgaag gaaccgctgc
     5341 tcaagcggac gcttgccggg cgctacctgg cgcggcgcgt cgaacaggtc gaacgcgagg
     5401 cggtcacgcg ggcgcgcctt gtcctggcgt gcagccacgc cgacgccgag caatttgagc
     5461 ggcgctacgg cgcgcccccc gaaaaactgc tccacatccc caacggcgtc gattgcgatg
     5521 cgatcgcccc ggtcgaggcc acgcgccgcc atatcctgcg ccgccgcctc ggcatccccg
     5581 accgcccgtt cgcgatcttc gtcggcagcc actacgggcc gaatatggac gccgccgaat
     5641 tcctcatccg gcagctcgcc ccgcagttga ccgaactgac cgtcggcatc atcggcggcg
     5701 tcggaccggc ctggcgcgaa cagcacggcg acgaactgcc cgccaacgtc cgcctgttcg
     5761 gtttcgtcga cgccgaacaa ctcgtccagc tttaccaatg cgccgatctg ggcctcaacc
     5821 cgatgcagca gggctccggc acgaatatca agatgctgga ctacatggcg gccgggctgc
     5881 cgatcgtcac gaccgagacc ggcgcccgcg gcctggcggg cgaaaacggc gtccactggt
     5941 gcaacgccac cgtgttcgag tttccccagg cggtgcgcga attgctggcc gcccccgccc
     6001 gcgcggccga gatgggtcgc gccgcgcgcc gcctcgccga ggaggtctac gactgggccc
     6061 gcatcagcct gcgcctgctg ctggggcgcg acgcggccgg cgccgaactc gtcgccacgc
     6121 tccgcgagga cagcgcatga agctcctcgt cgtttccacc taccctcccc tcgcctgcgg
     6181 catcggccgc tatgcggccc agcaggtcga tttcctgcgc cgcgaggggc attgggtcga
     6241 tgtcctgacg cccgccggct gcgacggcga tttccaggac gacctgtacg gccggtttcg
     6301 catcctcgcg ctgctcaaat acctctgggc ctacgacatg gcctacctgc acttcgcccc
     6361 gcagttcttc tacgacaccg ccagccgcgc cagccgtttc atgaccagct tcggctttct
     6421 gctcgtcatg ctgctggccg gcaagcgcat cacgttcatg atccatgaga ccggctacaa
     6481 gatcggccag gaggggcgcg gcctgctgcg ccatcgcatc gatcgctggt actggcgcct
     6541 ggccggccgc gtcgtcttcc actccacgcg cgaacgcgac actttctgct ggttctaccg
     6601 actgaaaacc acccgggcga atctcgaggt ctggccgcac gacaagtaca tggttcccct
     6661 ctggccgcac gaccgcgccg aagcacgccg gcgcctgagc ctggagcccg acaagctgtt
     6721 cttcctctgc atcggcttcg tccagccgca caagggcttc gaccgcgcgc tggaggcgtt
     6781 cctcaagacg ccgggcgagc atctgcgctt caagatcgtc ggctcggtcc ggatcaactg
     6841 ggacgtggcg caccagtacg cgcagcggct gcacgagctg gccgagcagg atgcccgcgc
     6901 cgagttcgtc gagtcgttcg tcaacgacga gatgttcgac atgtgggtcg ccgcggccga
     6961 ttacgtggtt ctaccctacc gcgagatctg gacctcaagc gtcgccgcgc gcgcgcggct
     7021 ctatggccgg ccgctcatcg ccgccgacac gggcggcctc gccgagcagt taaccgaggg
     7081 ctcgatgctc tttcgcgatg atgatgagct ggtcgaatgc ctgcgccgga tcgccgcccg
     7141 gaccaacccc tcccccgccg gcgaaccaac ccccacgccg cactgacccc aggaatccgt
     7201 cacccgtctc tcgctctaaa gtcgtctctc gtcctcggct ctcgcagatt tctgtttttt
     7261 ttgcttcggg cccctcccgc tcaaatcgac gcctgttgta cccgctccag tgaataaata
     7321 tgatgttcgt cctgatcggg gcagtaggcg taaaccttct tcccctgcag ggcctccggc
     7381 tcgatcgtca gttcatgctg ctcgccggtc gtgcgttcgt agagaatccg cacgggcttc
     7441 tcatgctcga tggcgtagcg caccagcttg ccgatctccg ccggttcgcg cgccgggttt
     7501 tcccccttgt atcccgacaa ggcccgggcc ttcggcatct gcgtcagcag ccggttgagc
     7561 tgcttgcggt atttccgcgt ttcctcgtcg atcgttttct tattggcttt ttcataattg
     7621 gcaatgaaat ttcgcgcgat cgattccgca tagaatttcg gattgaaatt ctcctgcgca
     7681 tgccccgaca accgctggaa cagcggctgg atccggtcct cgaagatcga cgcctccttc
     7741 tcctcctcca gcagaatccc gaattgcagg atcgccagca gatcgtacaa ttcctcttcg
     7801 cgcagcgtaa tctgaaacag cccttcattc gtcacgtaga ggctgtccga atcaacccgc
     7861 ggcagatagc cgaggcgctg cagctcccgg gtcagcttcc tgaaatcggt cgtccggctc
     7921 agcaggacga gccggtcgtc gaacacgtcc ttgatatacg gcgcgatctt gctgttcgcg
     7981 cgcaactcct ccaggtgatg ccggtcggcg acgcgcaggt agcccccggc catccccatg
     8041 tcgagctcgc cgtggcgcga ttcgcactcg ctgatcatct gacgcaccgt ctccggcacg
     8101 tccttgcgcg aacattcctg cagcagagcc agcatctgcc gcgccgtcaa cccggaatcc
     8161 aggccggcgc ggattgaatc atgcgagatc gtcaacgtcg tcatgacgtc gaccttcttg
     8221 atatcggcaa actggagcag cgtgaagaat ttgtccagtt gcagatcggg cggcgtgaca
     8281 atctcctgat tcggctgcac cgtgaaatac gtcgactgcg aggcgaacgg cagcgcccca
     8341 tccggcatgc gcgcgaacag cttttcgggg tcgagatacg ttcgcgtgag gatctccgcc
     8401 cccgtctccg agagccggaa catgaacatc agttgctcgt cgccgacgag ccgcgacgag
     8461 aacggccggc tcgggtcgta cggcgcgatc gcctcattcg gccggctgcc cagttggcgc
     8521 gcgacttcca gatccgacac ccccagcacc accagcccca gccagtagag cgtctccgcc
     8581 accaccgact ccatgatcag cgtcggatgg cggttatgct tgtcggcggg cgtcaggtcg
     8641 aagcgccccg gaatctcgat cgcgatctgc ggcagcaggc tctcggcgaa cgtttcaaaa
     8701 tcgatccacg tctcggacgg cagcttttcc agcatgcgca gcaccagctt gcgcaactcg
     8761 atgatgctga tcagattctg cgggtaattg tcggcgtgga ccacgtcgcc gtcgatgaat
     8821 tcctcgttcc attcgctcgt gttgagccag aactgataca gatcgcgata acaggcctgg
     8881 cccccctcca gccagcggcc gagcgaactg gacacgcgcc actgatcgcc gacggcgatg
     8941 atcagcttgc gcacgatcgc gaacagcgcc aggaacgaaa catacttgat cgtcttgttg
     9001 tggctcatca gcgggacgat ctttttcaga tcgttgcgcc ccaggctgcc gttgttgagc
     9061 atcttcaccg gattgcgctg aatgtaggcg cacaggatga ccaggtcgcg cagaatatta
     9121 ttggaattat caaggatgac gcccggccgc atccccccct cggccccctt gcgcgtggtc
     9181 gcttcgggcc cgctgtggct cagctcctcc agcgagcgct cgtcgcgctt gtagccgttc
     9241 tgaatcaccc acgccacgtc gcgcggcacc agcagcaggt tgttgtattt gttcggggcc
     9301 gaacgcagca cgaacaccag cccgctctgc tcgaccagct cgcgcagctt atccgcccgt
     9361 tgatggtcga atttcttgtg cacgccggcg tcgagcaggt ccttccacgc gcagacgccg
     9421 ttcttctgca ggatctgctt gaacatttcc acctgcagcg ggttgagccg tgcaagcacc
     9481 tgctgcaggc tcttcggatc gagcagccgc ttgcgcagga aatgcaccag cgcctgcttg
     9541 cgccgcgtcg gcgcgcttgt ctcgagcgta tgcttcgtga tcgcgcgcag atcgtcggtg
     9601 cccagcgcct gcagatagta gccgagaaat ccctgccagt aggggggcag atccaccagc
     9661 cgcacgaacg gctcgggaat accaatcagg tcgtggctga tcagcttgtc gcgcagccgc
     9721 tcgcgccaca gaaaaccccg ctcgaacagc ggggcgaggg cttcgttcat cgcatcggcc
     9781 gtttcgaacc ggcaacgttt gcggaattcc tcaatcggca actccccgcc atgaatcgcc
     9841 agaaaataaa cccagtcgcg ctgcgccggc gtcaactggt cgaacgcgtt gcggaaattg
     9901 gacgcaacct gcattcgcgg gtagaggtac tcggccagcc ccgcggcgtc cagcgccggc
     9961 tcgcccgcct ccagttgcag cccccaccag tcggcaatcg cgcgcagctg atccagattg
    10021 cattcgcgca gatgagtctt cagtttcatg atggttcggc cggaacggga tgcccctccc
    10081 cttccggcgc cctccggtca attcatggcc gcacggccca ggtcggcgtg atccgtcgcc
    10141 gcatcctcct ccggttccag ttcatccaga ctgctgacga tccggtagcg atacccctgc
    10201 tcggtcagga acaactggcg cttgaccgcg aaatcccgct cgcgcgtatc ctgcgtcacc
    10261 agcgtgtaga agtgcgccat gttgccgttg gccttgggcc gcaggatccg ccccagccgc
    10321 tgcgcctcct cctgccgcga accgaacgtc ccggaaatct ggatcgccac ggcggcgtcg
    10381 ggcaggtcga tcgaaaagtt cgccaccttg ctgacgatca gcacatggat cacgccattg
    10441 cgaaaatcgg cgtaaatctg ttcgcgctcg cggttgggcg tcttgccggt aatgatcggc
    10501 gccgaaaatt ccgcggccac cttctccaac tggtccagat actgtccgat gatcagcacc
    10561 tgatcgtcgg ggtgccgccg gatgatctcg cgcgcgacct gaatcttctc atcattttcc
    10621 gacgcgatgc ggaacttcgc cttcgcatcg gccaccgcat actcgtgccg gacatgctcg
    10681 ggcagcggaa tccgcacctc ggtgcatagc gcctgcgcga tccacccctg gcgctccagc
    10741 gtcttccacg ggatatcgat cttcttcggc ccgatcagcg agaagacatc cgactccttg
    10801 ccgtcctcgc gcagcagcgt cgccgtcagc cccagccggc gctttccctg gatccccgca
    10861 accgcccgga acaccggcgc cggcagcagg tgaacctcat cgtaaatgat cagcccccac
    10921 gcgcgccggt tgaacagctc catgtggacg aactcttctt cgcgccgctt gcgataggtc
    10981 aggatctgat atgtcgtgat ggtgaccggc cggatctcct tgacctcgcc gctgtattcg
    11041 ccaatctcgt cgggcgtcag cgtcgtcttg tccaggattt catcgcgcca ctgccgcaac
    11101 gccgtgatgt tggtcacgac gatcagcgtc tgctggccga tcgcctgcag aatgcccagc
    11161 ccgatgatcg tcttgcccgc cccgcacggc agcaccagca cgcccgcgcc gccgctggcc
    11221 cggccgtact gatgaaacac cgtgatcgcc tcgcgctggt attcgcgcgg ggcgaacggt
    11281 tcgcccgccg ccgtccgctc gcgcagcgcg acctcgagcc gttcgccttc ctcgtagcct
    11341 gccaggtcct cgaccggaaa ttccttcttg atcagcgcct gcttgaggaa cccgcgctcg
    11401 ttgggcttga cctgcgcgct ggtcctggtc agccgcttct caaagtagcg gcgaatccgc
    11461 tcgtcggcgt agaactgctc cagcagcagg gcatcgtcgc attcgagcac cagcgcgtcg
    11521 ccctgctgca ccagccgcaa ccggccgtag cgcgaaacat actcgcgaat gtcgtattcg
    11581 atattctggg gaatcgcgta gcggctgtag gcgcgcagac tggccaggat ctcctcggcc
    11641 gtcagccccg cgctggccgc gttccacagc gacagcggcg tgatgcgata ggtgtgaaaa
    11701 tgctcgggcg acgtcacaat ctccgcgaac gccgccagga cgtcgcgcgc cgcttcatac
    11761 aggggatggt ccgtctccaa cagcatcgtg cggtcggatt ggacgatgag cggcttttcc
    11821 tgagacatca tggggctggc gggatcaacg atgagtattg cgttcaaggc cgaccgacga
    11881 ccctggaccg gtaagagatt cggcgcgctc cagccggccg cttggagggc ggtgttcgct
    11941 tctctccccc agaaacaggc ccgcagagtt tgattatctc tcccccaaag agagaggtca
    12001 aagtcttttt cccaaccaca caattcgtcc ctcgtcctcg tgatcggcat tcctgccaaa
    12061 tcgcgttgca aaccctcccc ccatcaacga tcatgccaaa ggcgtcccct ggcccacgcg
    12121 caatgcccgc cccacgggga cgccgctctc atctccgccc cgcgcgacga tcctcgatgc
    12181 atgccgttcg caacctgctt gtcaacctcg tccggcgcga tctgaccctc cgctatcgtt
    12241 cgaccgtgct gggcttcttc tggtccttcg tcaagccgct tgccctgact gccatcttct
    12301 acatcgtctt cacctatgtc ctgcgcatcc cgctgcgcga agcgcgcgtc ccgttcgtcc
    12361 tgcacctgct gaccggcatg ctcgcctgga ccttcttcgc cggcgccacc ggcgaggcga
    12421 tggacgtcat cctcgccaac gccaacctga tcaagaaggt ccgtctgccg cttgcggtct
    12481 tccccctggc cacggtctgc tcgcatctgg tccacttctt tctggcgctg ctcgtccttg
    12541 tcggcctgct gatcgtcgcc gatctgcccc ccacaccggc gatcctgctc gttctgccca
    12601 tcgcggccct ccagtttctg ctggtgctcg ccgtggcgtt cgtgctgtcc gcgctcaatg
    12661 tcttctatcg cgacattgcc tcggtgtggg aggtgctgac gaccgcctgg ttttacgcca
    12721 cgccgatcat ctatccggtc tacatggcgc tggaggaaat cgaccggcgc ggctggggct
    12781 ggctccggct cccctacctg gccaacccga tgaccccgct ggtcctggcc taccgccgcg
    12841 tgctgctcta tgccggcgtc ggcgacacgc cccaggcgcc ccgcctgctg gaaatccctg
    12901 atttcgccct gctggcgggt
//
LOCUS       DAOPCO010000090        12765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4894_length_12765_cov_4.884500, whole genome shotgun sequence.
ACCESSION   DAOPCO010000090 DAOPCO010000000
VERSION     DAOPCO010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4894_length_12765_cov_4.884500"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PLS90_08030"
     CDS             <1..535
                     /locus_tag="PLS90_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02393.1"
                     /translation="APGEIALIAAGAAADPPGWLPAPPAPPPRVFHEPGRDVAAWAAW
                     AEPQALENFPRAAGGTSGRLALAAADGVALLAPGTRFEPGVGPTPRDNTIDGVNYPRG
                     NPSPACPINRLGALDSPATKAGEREPAAGMGLTRIRPGMNTASDAAFAVLPVTPGRSE
                     LIPAPQRTGLTDWPCYH"
     gene            757..1917
                     /locus_tag="PLS90_08035"
     CDS             757..1917
                     /locus_tag="PLS90_08035"
                     /inference="COORDINATES: protein motif:HMM:NF022818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HPK02394.1"
                     /translation="MALHPDQPQALAPGGSRRERPHAPPPGFWNRLNARLTPRLRRLL
                     VVLLIFLTALAAYLVGYEWTAGYHPVRRAAMLRASGEAWLYDLNPLRIDADQLFHSPL
                     RADTIPALVDPELLSAADAAFLDDDDLVLGVALDGAARAYPLLIIEWHELVNDTLAGH
                     PLLVVHQPFSGATAVYTRRIGPTVPEFGIGSLIFNSRILLYERRAEGRGQSLWDGMTG
                     QAVSGARAAEGAQLARLPFERCSWRAWRARHPDTTVVGGNQGLERPYRAPFFRIYEFN
                     DQVYWPMPEPSPARPDLGPKELMFLLWVGGRATPAAFALSDLAQTLEHRVDVAIAGRR
                     VRLEYDPAGQLVRVHPLGGDVEDLRWAYAYRFTLDAAWPAAPIWRPRFTYSQ"
     gene            complement(1921..2670)
                     /locus_tag="PLS90_08040"
     CDS             complement(1921..2670)
                     /locus_tag="PLS90_08040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK02395.1"
                     /translation="MKGPSTSIRSAMLALCGLALLVGGCSREPRHLDEALAGQPAGVY
                     AYFQTDLGDFLARLFIERAPRTTANFIGLAEGTKEWVEPKSEKWVTGPFYDGLLFFKV
                     IDNEFILTGDPLHLGIGGPGYQLDPEFHPELRHDRAGLLTVPPQGEEEKASGSQFMIT
                     LGPRPTADDRSSIFGEVVWGMETVRAIGRIPTHMDNRPVKDIKLRRVTIHRVGGAREA
                     TGADDAPTTAPATTTPETTAAAESTEVAPPD"
     gene            complement(3310..3849)
                     /locus_tag="PLS90_08045"
     CDS             complement(3310..3849)
                     /locus_tag="PLS90_08045"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPK02396.1"
                     /translation="MQRIQRIPASSRTALLLLALPVVILSLAAARTEPTAVGVVDVDL
                     VRERSAHVQAQLRALASPVQQIQDSLKAKQAELQQLLSEYAAQQSAATEEVNQRRVER
                     IREVSREAEELSRQFEDAWNQAEQAGLAPLRDTIFAAVEQEAAARGMRVILRSDSVVY
                     MDDAQDITQAVVSRLDANR"
     gene            4179..4532
                     /locus_tag="PLS90_08050"
     CDS             4179..4532
                     /locus_tag="PLS90_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02397.1"
                     /translation="MNLENYRLSFPLILVEVPPSANARARVFPDRAALLSHARSRGGN
                     DLNGKPERNDLDLLEAVTADLTAYDLVQSAEEAREELLRLEQRAIPSCVQQAARAREL
                     RREAERLGWITKTID"
     gene            complement(4607..5716)
                     /locus_tag="PLS90_08055"
     CDS             complement(4607..5716)
                     /locus_tag="PLS90_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02398.1"
                     /translation="MTRLRRCLLCALLAAAGCADGPPDGSLRRLATPAAPYASGVTVL
                     AEQVAVLQLPAPPRHLVLSDAALYVFLNRHGLATLDLSDPARPRLAHWLQGAMANDDQ
                     RGHHFLNGILEPERARLVVADRSQGITFIDVSQPLAPRVERTIEFPGDLPTAILPDGN
                     GYYVAGGGRGLFHLAEGWSEGGPIKPVLTDFDHVKDLRWLAPNLLVFSDHVQNGLQVL
                     DVSRRDAPRALALFSLRQYCDSLQVIGQMVALQVRELGVVLVNLEKPERPWIQSVLPT
                     GGISSLTCMARWGEHRLLVGDTAGTIQILDLEDQARPCWVGRILARSSIACLETRGDL
                     LYVGLNHEPAAEAIRPGHQFAVYRLTARRVSDPEG"
     gene            complement(5772..6230)
                     /locus_tag="PLS90_08060"
     CDS             complement(5772..6230)
                     /locus_tag="PLS90_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02399.1"
                     /translation="MARLNLLRARADKPSSTEADGRRRVLLIDDERDLCTLLQMALEP
                     RGYVVEAAHDGIEGWNRIEQSPPALVVLDIKMPKMNGYQLLARLQRDERTRDIPVVVF
                     TSVTEGAPELTEAEWAERLEVGAFIAKPSDPERVVDAVDRLLQPDPAAQG"
     gene            6688..7770
                     /locus_tag="PLS90_08065"
     CDS             6688..7770
                     /locus_tag="PLS90_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02400.1"
                     /translation="MSKYRIMVIDDDDDTRRMLSFILRDRFEVVEAADGLEALSKLET
                     HEPDLAIIDVMMPLMDGYQVCEAIRKHARFNNMQVMFLSAYGTKENIRKGYASGANLF
                     LTKPVEPERVLKNIDFTLRHEPPPLRTKRYTLEQLRELEERQARDGQAVSRTAETSDR
                     RDHGFDVFSEDLPLAGGQPAGAAALMARLLIVDDDVEMLQMLDIALREEFEITTALNG
                     LEAIEHLVEYDPDLMLIDIMMPKMNGYQLLQSVRRNAYFRNLPVIIVSAKSSPRDREY
                     AARLGATGFLAKPFQIDELLETVRPLLAECRIKKKRVALVDIKAKAFKQLRDREERKR
                     DRAGGPAPIQFRPVGPEKRDADPPRP"
     gene            complement(7781..9475)
                     /locus_tag="PLS90_08070"
     CDS             complement(7781..9475)
                     /locus_tag="PLS90_08070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02401.1"
                     /translation="MSQASAAGYLELRDGAATSPLAGDLRRLGLLLAGLTLVRVILAP
                     LFPMLSGEAYYWLWSKHLAPGYFDHPPMVGVMSAIFFGWVEASELAARSGPIILAVLS
                     SLIVYRLARDLFPSGPTAWRGAALFSVTPIFFANGLLTQPDNSLVLFMTLTWFAFWRG
                     CGGAAPDPADARRPERLGWWALAGVAAGCALLSKFMAWVLLPPLWAFLAVSRPHRHLL
                     RSPGPWIAAGLALLVLSPNLIWNAQHEWINYAFQWRRSDLPEAEFEAKNIPLYLFGPL
                     LTLSPLLYFALLRGVVRGWSLWRREHDARWLFLLAAGVPLPLFLGLLSVMVTISLHWP
                     AAGYIPLILLAVGLIHEQGMFGARYVRWLTRLCVGTTLFAVALALTVLALPTSVPWED
                     LNEDLAEIKAELLGWEAIGGRVRAERQAMEDDSGNRTVIMADNWHLAGPLAFYSAAYG
                     DVFAVVDEDAHNFEIWRQEQGGLKGADAVFVIKKSKPNYKHSKLEKKYDKYHRFLDPL
                     FEDVDAVSSVLVYADDGSTAEYYGVDVSRPRLREFLIFRCYGFKGRLARADDRDDD"
     gene            complement(9529..11571)
                     /locus_tag="PLS90_08075"
     CDS             complement(9529..11571)
                     /locus_tag="PLS90_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK02402.1"
                     /translation="MNATSSAPPVPEPRRAWRALTDGQEARASADQLLCGLGLLFWLA
                     MFGWLWLGAPTLGRVLGLLWFVLGALWPMGYFYSLALALPWFGNNPGGPHHLYLVEMG
                     LMGLVSGHLTTRLLLWVSPKRSRLDAAVWFFVLYSCVCVFPQWRYLWAELLTYPRQFL
                     FVIHNHYGTSYTFGLQLALKLALSAGLYVALRDRRLPALRLLRLWWLLVVSLAASALL
                     GVGEYFDWLSLAWWRAENPDISRFGYDRLQSLYWHSGWYAQYLAALAPGALALAVIGR
                     VARHPERAALLSRRPFRLAAIRVRWFYWSLVLLLCLTQLMTQQRGGWIALTAGLAVVI
                     AAVMITNGPERLRWGRTALAVGLTVTTIVLLAGSVALFSPVFRHRVTELFAYEDRMDI
                     WLSAIGLFRIHPLVGVGLGNYHRVHLGVYPEGHPYFHIDKVAPHSLYLHVLSERGLIG
                     LIALLLIIAGVVWGAWRIVKESRDDLARWGTGLALLGGVVAILFYGIFQETFYIRTVD
                     LIFWMFVALASWSPALALEPAAGAPPRWKRAVWIGLLILAAGGVVWGHRFQLQPVVFY
                     LDGETFLTAGQYAKIDVPRDARRVAIRIVGYDPAIPQSPVTITFRAAGEELAREVLDT
                     RRAREIELTLPPDRRPGEPIVAEVSRTWNPLLYDMRWFPLPRVGVLYQPPRVLETE"
     gene            11793..12512
                     /locus_tag="PLS90_08080"
     CDS             11793..12512
                     /locus_tag="PLS90_08080"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HPK02403.1"
                     /translation="MAPFSRIARAVALLALTIGVLPLMGCLRTTHKVETYHKIDAHIV
                     LDVRQIRDEAEQVETYVRSDDPAPQAGGDQPVSRVWTEPLPDSDSLFARVFRFSPVAL
                     AVTPQEADAAKKARKERSKQVDEALTKHWVGEDDHGYLAVRLGKDEDKELRARVDALV
                     KAENRDRKTIYQEIAERKGNGKELLEAVEIIFADEIRNKLKKGQLFRAPRDAKGFDVF
                     RDSPLGRKYPDARPGDWLEKR"
BASE COUNT         2210 a         4593 c         4084 g         1878 t
ORIGIN      
        1 cgcgccggga gagatcgcgc tgatcgccgc gggcgccgcc gccgatccgc cgggctggct
       61 gcccgccccg cccgcgccgc cgccgcgcgt attccacgaa ccgggccgcg acgtcgccgc
      121 gtgggccgca tgggccgagc cgcaagcgct cgagaatttc ccccgcgccg ccggaggcac
      181 gtccggcagg ctggcgctgg ccgccgccga cggcgtcgcg ctcctcgcgc cgggaacgcg
      241 ctttgagccc ggcgtgggcc cgaccccccg cgacaacacg atcgacggcg ttaactaccc
      301 gcgcggcaac ccgagccccg cctgtccgat caaccgcctg ggggcattgg actcgcccgc
      361 aacgaaggcc ggcgagcgcg agcccgccgc cggcatgggc ctgacgcgca tccgccccgg
      421 gatgaacacc gcctccgacg ccgcattcgc cgtgctgccg gtcacccccg gccgcagtga
      481 attgatcccg gcgccgcagc gaacggggct gaccgactgg ccgtgctacc actgacgccg
      541 cgctacaaaa ggagcgaggt cgcgcccgct ttgtggagtg cggcggcaga gcgcggcgcc
      601 agccgcgcgc cgacgccgct ttaggatcga ccacccgcgg ccgattccgc atgatcccac
      661 ggcctggatc tccaaccact ttcccgatct cttgctctcg cggcactcgg ttatgccgct
      721 tccctcggcc cgcacctcac gctagcatgg tcccccatgg cacttcatcc ggatcaaccg
      781 caggcattgg ctcccggcgg ctcgcggcgg gagcgcccgc atgctccccc acccggcttc
      841 tggaaccgcc tcaatgcccg actcaccccc cgcctccggc ggctgctcgt cgtcctcctg
      901 attttcctga ccgccctggc cgcctacctg gtcggctatg agtggaccgc cggctaccac
      961 ccggtgcggc gcgcggcgat gctgcgcgcc tcgggcgagg cctggctcta cgacctgaac
     1021 cccctgcgaa tcgacgcgga tcagctcttc cacagccctc tgcgggccga taccattccg
     1081 gccctggttg accccgaact gctcagcgcc gccgacgccg cctttctcga cgacgacgat
     1141 ctggtgcttg gcgtggcgct ggacggcgcg gcccgcgcct atcccctgct gattatcgaa
     1201 tggcatgagc tggtgaacga cacgctggcg ggccacccgc tgcttgtcgt ccatcaaccc
     1261 ttcagcggcg cgaccgccgt ctacacccgg cggatcggcc cgacggttcc cgaattcggc
     1321 atcggcagcc tgatcttcaa cagccggatt ctgctctatg aacgccgggc cgagggccgc
     1381 ggccagtccc tctgggacgg catgaccggc caggcggtca gcggcgcgcg cgcggcggag
     1441 ggggcgcagt tggcgcgcct cccgttcgaa cgctgttcct ggcgcgcctg gcgcgcgcgc
     1501 caccccgaca ccaccgtcgt cggcggcaat cagggtctgg aacgcccgta tcgcgccccg
     1561 ttcttccgca tctacgaatt caacgatcag gtctactggc cgatgcccga accatccccc
     1621 gcgcggcccg acctgggtcc caaggaactg atgttcctgc tctgggtcgg cggccgcgcg
     1681 acgcccgcgg ccttcgcgct gagcgatctg gcgcaaacgc tcgagcatcg ggtcgacgtc
     1741 gccatcgcgg gccggcgcgt ccgcctggaa tatgaccccg ccggccagct cgtgcgcgtc
     1801 catcccctcg gcggcgacgt ggaagacctg cgctgggcct acgcctaccg gttcacgctc
     1861 gatgccgcct ggcccgcggc gccgatctgg cggccccggt tcacctattc gcagtaagcc
     1921 tcagtccggc ggcgcaacct cggtcgattc ggcggcggcg gtcgtctccg gcgtcgtcgt
     1981 cgccggcgcg gtcgtgggcg cgtcgtccgc cccggttgcc tcgcgcgccc cgccgacgcg
     2041 gtggatcgtc acccgcctca gtttgatatc cttgaccggg cggttgtcca tgtgggtcgg
     2101 gatgcggccg atcgcccgga ccgtttccat cccccagacg acctcgccga aaatggagct
     2161 gcggtcgtcg gccgtcggcc gcggccccag cgtgatcatg aactggctcc ccgaggcctt
     2221 ctcttcctcc ccctgcgggg ggaccgtcaa caggcccgcg cggtcgtggc gcagctcggg
     2281 gtggaattcc gggtcgagct ggtagcccgg cccgccgatg cccaggtgca gcggatcgcc
     2341 cgtcaggatg aactcattgt cgatgacctt gaagaacagc agcccgtcat agaacgggcc
     2401 ggtgacccac ttttcggact tcggctcaac ccattctttc gtcccctcgg ccaggccgat
     2461 gaagttggcc gtcgtcctgg gcgcgcgctc gatgaacagc cgcgccagaa aatcgcccag
     2521 atccgtctga aaatacgcgt agacgcccgc cggctggccc gccagcgcct catccaggtg
     2581 acgcggctcc cggctgcacc cgcccacgag cagtgcgagc ccgcacaacg caagcatcgc
     2641 gcttcggatg gaggtggaag gacctttcat gcttcgacct ttcatcacgg caacttcttc
     2701 tcccctatag aacagaatcc gccgcgcgct gtccagcggc ggctggcccg gcgcccggcg
     2761 gcggggcctt ggctttcgcc cccgccgccc gcgggcgcgg aacgaccggc gccgccggca
     2821 ttcagtcata aatgggattg gctgaaaatg agaactcccg gggagtggac cgcgcgggac
     2881 ccgtccccgg gagaggtgct tgggttgcgg gcgagagggc tgcgtcaggg gatggagtta
     2941 ggtatggatg cctttgtctc gcgttcgtga gcgcacctga cgctgccagt ccaatcggta
     3001 gagtggacac ggtttagcct tcaggtactg ctccttgtgt ggatgcgaac aataaaccag
     3061 gtcgggccgt tgttcgtgac gatgatgata gaggcaatcg cacagggtgc agttgacctc
     3121 gacccgatgt gccgtcatga gccttgtcgg ttactccttt ccaggtgtgc gtcgtaggcg
     3181 gcgggggcgc gtgtgcgatt cccggccgtg gttcccgcgc ggtccctcgc ccggaccgtg
     3241 ctacaagccc tcacgggcat tatagggcgc gcgggcccgg cgcgtcacgt aaatcccccc
     3301 gggcgcagct taccgattcg catcgagccg gctgacgacc gcctgcgtga tgtcctgcgc
     3361 gtcgtccatg taaacgacgc tgtcgctgcg cagaatcacg cgcatgccgc gcgccgccgc
     3421 ctcctgctcc accgcagcga agatcgtgtc gcgcagcggc gccagacccg cctgctcggc
     3481 ctggttccag gcgtcttcaa actgccgcga cagctcctcg gcttcccgcg acacctcgcg
     3541 gatccgctcc acccgccgct ggttcacttc ctcggtggcg gccgactgct gcgccgcata
     3601 ctcgctcagc agttgctgca gctccgcctg cttcgctttc aggctgtcct ggatctgctg
     3661 gacggggctc gccagcgcgc gcaactgcgc ctgcacatgc gccgagcgtt cgcggaccag
     3721 gtccacgtcg acgacgccga ccgccgtcgg ctcggtgcgc gcggccgcca gcgacaggat
     3781 cacgacgggg agggccagca ggagcagggc cgtccgcgaa ctggccggga ttcgttgaat
     3841 gcgttgcatg gcttgacctc gagtggactg gcccccgcga cgggggccgg gcggctcgtt
     3901 cgccgcgacc ggcgggatcg gaccgcatcg aatcagccca ccatcatgcc cggggtttgg
     3961 caatgggaat tcgaaccggc gatggggagt gcggtggcag agcgcggcgc cggccgcgcg
     4021 tcgacgccgc tttaattcac gtggccgcag ccgattccga tcgctcggct ttcccccccg
     4081 acattccttt tctcgtcaaa tgataaatct tgacactctt ccgaccaacc tgtgaagatg
     4141 ctattcgtta gattgttaaa attcggacaa atttgaccgt gaacttagaa aattacaggc
     4201 tgagttttcc gctcatcctc gtggaagtgc cgccctcggc caacgcccgc gcgcgcgtct
     4261 ttcccgaccg cgcggcgttg ctgtcgcacg ctcgatcgcg cggcggcaac gacctgaacg
     4321 gcaaacccga acgcaatgac ctcgacctcc tcgaagccgt caccgccgac ctgaccgcct
     4381 acgacctggt gcagagcgcc gaagaagccc gcgaggaact gctccgcctg gagcaacggg
     4441 ccatcccgtc ctgcgtccaa caggccgcgc gcgcccgcga actgcgccgc gaagccgaac
     4501 ggctgggctg gatcaccaaa accatcgact gaagccggaa acacctggcg ccgatcggac
     4561 gcgccccgcc ctccaagccg ccgaacaccg cggcgtgata aaccgcttac ccttccggat
     4621 cgctgacccg ccgggcggtc aaccgataga cggcgaactg atgaccgggc cggatcgcct
     4681 cggccgccgg ctcatgattc aagccgacgt agagcaggtc gccccgcgtc tccaggcagg
     4741 cgatcgacga gcgcgcgaga atccgcccga cccagcacgg ccgcgcctgg tcttccagat
     4801 cgaggatctg tatcgtcccg gccgtgtcgc cgaccagcag ccggtgctcc ccccaccgcg
     4861 ccatgcaggt cagcgacgaa atcccgcccg tcggcagaac actctgaatc cagggacgct
     4921 cgggtttctc tagattcacc agcaccacgc cgagctcgcg cacctgcaag gccaccatct
     4981 ggccgatcac ctgcaggctg tcgcaatact gcctcaacga aaacaacgcc agtgcgcgcg
     5041 gggcgtcgcg ccggctcacg tccagcactt gcagcccgtt ctggacgtga tccgagaaga
     5101 cgagtagatt gggcgccagc cagcgcagat ccttgacgtg gtcgaagtcg gtcaggaccg
     5161 gcttgatcgg ccccccctcg ctccagccct cggcgaggtg aaacagtccc cgcccgcccc
     5221 cggccacgta gtagccgttg ccgtcgggca ggatcgccgt cgggaggtcg ccagggaact
     5281 caatcgtccg ctcgacccgc ggcgcgagag gctgcgagac gtcgatgaac gtgatcccct
     5341 gggaccggtc ggccaccacc aggcgcgccc gttcgggctc cagaataccg ttgaggaaat
     5401 gatggccgcg ctggtcgtcg ttcgccattg ccccttgcag ccagtgggcc agacgcggcc
     5461 gggcggggtc gctcaggtcg agcgtcgcca gcccatggcg attgagaaag acgtagagcg
     5521 ccgcgtcgga cagcaccaga tgccgcggcg gcgccggcaa ctggagcacc gccacctgct
     5581 cggcgagcac cgtgacgccg ctcgcgtagg gcgccgcggg cgtcgcgagc cggcgcaggc
     5641 tgccgtcggg cggtccgtcg gcgcacccgg ccgccgcgag cagcgcgcag agcaggcagc
     5701 ggcgcaatcg agtcatgcgg gcagtttacg gcggatgccg ttcaatcgga cagcgcaatc
     5761 gcgcgggccg gtcagccctg ggcggcggga tcgggctgca acaggcggtc gacggcgtcg
     5821 acgacgcgct caggatcgct cggtttggcg atgaatgccc ccacctccag ccgttcggcc
     5881 cattccgcct cggtcagctc gggcgcccct tcggtcacgc tcgtgaagac gacgaccggg
     5941 atgtcgcgcg tgcgttcgtc gcgctgcagc cgcgccagca gttgatatcc gttcatcttc
     6001 ggcatcttga tgtcgaggac gaccagcgcg ggagggctct gttcgatccg gttccagccc
     6061 tcgatgccgt cgtgcgccgc ctcgacgacg tagccgcgcg gctcgagcgc catctgcagc
     6121 agcgtgcaga gatcccgttc gtcgtcgatc agcaatacgc gccgacgccc gtcggcctcg
     6181 gtcgagctgg gcttgtccgc ccgggcccgc agcaggttca aacgcgccat gacacgcctc
     6241 atgcgatgat tgaactggct ggccgcggcg ggggcgcgcg gccgaaaccc caaagcttag
     6301 agtatagtcc gcccgcgcgc cggcggcttg ggaaaaaacg ggcccacccc gcgcgcatct
     6361 caacaccgtt cgcgccgccc ggcattgggt ggcgcttacc tgagcagagc gcggggccga
     6421 ggcatctcct gtggagggca gcggcaaagc gcggcgcgag ccgcgcgtcg acactgcttt
     6481 ggagcttata acgttggctg atgctgaccg atccacaacg gcgccgccag gcgctgggtg
     6541 cttcccactg cctcactcca ggaggacatc atcgaacggt cttgcgttca gaagacgaag
     6601 gcagtacgat ttacctacct ggtcgtgtca agggcgggtc ccctcccgct ggatccatcg
     6661 gatctccgat caccgaaagg tggcgggatg agcaagtatc gcatcatggt catcgatgac
     6721 gacgacgaca cgcgtcggat gctctcgttc atcctgcgcg accggttcga agtcgtcgag
     6781 gccgccgatg gtctcgaagc cctcagcaag ctcgaaaccc acgaaccgga cctcgccatc
     6841 atcgatgtca tgatgccgct gatggacggc tatcaggtct gcgaagccat ccgcaagcat
     6901 gcccggttca acaatatgca ggtcatgttc ctctcggcct acgggacgaa ggagaacatc
     6961 aggaaaggct atgcctccgg cgccaacctg ttcctgacca aaccggtcga acccgagcgc
     7021 gtgctgaaaa acatcgactt cacgctgcgg cacgagccgc cccccctgcg caccaaacgc
     7081 tacacgctgg agcagctccg cgagttggag gagcgccagg cgcgcgacgg ccaggccgtc
     7141 tcccgaacgg ccgagacctc cgaccggcgc gatcacggct tcgacgtctt cagcgaggac
     7201 ctccccctgg ccggcggcca gccggccggc gccgccgcgc tgatggcgcg cctgctgatc
     7261 gtcgacgacg atgtcgaaat gctgcagatg ctcgacatcg ccctgcgcga ggaatttgaa
     7321 atcacgacgg cgctcaacgg gctggaggcg atcgaacacc tggtcgaata tgaccccgac
     7381 ctgatgctga tcgacatcat gatgcccaag atgaacggct atcaattgct tcagtcggtc
     7441 cgccgcaacg cctatttccg caacctgccg gtgatcatcg tctcggccaa gtccagcccg
     7501 cgcgaccggg agtatgcggc ccggctgggc gccaccgggt tcctggccaa gccgttccag
     7561 atcgacgagt tgctggaaac ggtccgcccg ctgctggccg aatgccggat caagaagaaa
     7621 cgcgtggcgc tggtcgacat caaggccaag gcgttcaagc aactgcgcga ccgcgaagag
     7681 cgcaagcgcg accgcgccgg cgggccggcg ccgattcagt tccgcccggt cggccccgaa
     7741 aagcgcgacg cggacccgcc acgaccgtag ttcccttcac tcaatcgtcg tcgcggtcat
     7801 ccgcccgcgc cagccgcccc ttgaacccgt aacagcggaa aatcaggaac tcgcgcagcc
     7861 gcggccgcga cacgtcgacg ccgtaatact ccgcggtgct cccgtcgtcg gcatagacca
     7921 gaacggacga caccgcgtcg acgtcctcga acaacgggtc cagaaagcgg tgatacttgt
     7981 cgtacttctt ctccagcttg ctgtgcttgt agttgggctt gcttttcttg atcacgaaca
     8041 ccgcgtcggc gcccttcagg ccgccctgtt cctgccgcca gatctcgaaa ttgtgcgcgt
     8101 cctcgtcgac gacggcgaag acgtcgccgt aggcggcgga atagaacgcc agcggacccg
     8161 ccagatgcca gttgtcggcc atgatgaccg tccggttgcc gctgtcgtcc tccatcgcct
     8221 gccgttcggc gcggactcgc ccgccgatcg cctcccagcc cagcaactcc gccttgattt
     8281 ccgccaggtc ctcgttgagg tcctcccagg ggacgctggt gggcaacgcc agcaccgtca
     8341 aggccagcgc gaccgcgaac agcgtcgtgc cgacgcacag ccgcgtcaac cagcggacat
     8401 agcgggcgcc gaacatcccc tgttcgtgaa tcagcccgac agccagcagg atcagcggaa
     8461 tgtagccggc cgccggccag tgcaggctga tcgtgaccat caccgacagc agccccagaa
     8521 acagcggcag cggcacaccg gccgccagca ggaacagcca gcgcgcgtcg tgttcgcgcc
     8581 gccacaggct ccatccgcgg acaacccccc gcagcagggc gaaatagagc agcggcgaga
     8641 gcgtcagcag cggaccgaac aggtacagcg ggatgttctt ggcttcgaac tcggcctccg
     8701 gcaggtcgga gcgccgccac tggaacgcgt agttgatcca ctcgtgctgg gcgttccaga
     8761 tcagattggg cgagagaacc agcagcgcga ggcccgccgc gatccacggc ccgggcgagc
     8821 ggagcaggtg gcggtgcgga cgcgagacgg cgaggaacgc ccacagcggc ggcagcagca
     8881 cccacgccat gaatttgctc agcagcgcgc agccggcggc gacgcccgcc agcgcccacc
     8941 agccgagtcg ctccggccgc cgggcatcgg ccgggtcggg cgccgcgccc ccgcaaccgc
     9001 gccagaacgc gaaccaggtc aacgtcatga acagcaccag gctgttgtcg ggctgcgtca
     9061 gcaggccgtt ggcgaagaag atcggcgtca ccgagaacag cgccgcgccg cgccatgccg
     9121 tcgggccgga cgggaacaga tcgcgcgcca gccggtagac gatcagcgag ctgagtaccg
     9181 ccaggatgat cgggccggac cgcgccgcca gctcgctggc ctcgacccag ccgaagaaaa
     9241 tcgcgctcat cacgccgacc atcggcggat gatcgaaata gcccggcgcc aggtgcttgc
     9301 tccacagcca gtaataggct tcgcccgaca gcatcgggaa cagcggcgcc aggatgaccc
     9361 ggacgagcgt caacccggcc agcagcagcc ccagccgccg caggtcgccg gccagcggcg
     9421 acgtcgcggc tccatccctg agttccagat agccggcggc ggaggcttgc gacatggatg
     9481 cgggctccag gggagatagc aacggatggc gcgcggcccc ggcgaggtct attccgtctc
     9541 gaggacgcgc gggggctgat acagcacgcc gacgcgcggc agtggaaacc accgcatgtc
     9601 gtagagcagt ggattccaag tgcgcgagac ctcggccacg atcggctcgc cggggcggcg
     9661 gtcgggcggc aacgtcagct cgatctcgcg cgcgcgccgc gtgtccagga cttcccgcgc
     9721 cagctcctcg cccgccgcgc ggaacgtgat cgtgaccggc gactgcggaa tcgccggatc
     9781 gtaacccacg atgcgaatcg ccacccgccg ggcgtcgcgc ggaacgtcga ttttggcata
     9841 ctgccccgct gtaagaaagg tctcgccgtc gagatagaac acgaccggct gcaactggaa
     9901 gcgatgcccc cagacgacgc cccccgcggc cagaatgagc agcccgatcc acaccgcgcg
     9961 cttccaccgc ggcggcgcgc cggccgccgg ttccagcgcc agcgccggcg accagcttgc
    10021 cagcgcgacg aacatccaga agatcaggtc gaccgtccgg atatagaacg tttcctggaa
    10081 aatgccgtag aacaggatcg ccacgacgcc gcccaggagc gccaggccgg ttccccagcg
    10141 cgccagatcg tcgcgcgact ccttcacgat gcgccaggcg ccccacacga cgcccgcgat
    10201 gatcagcagc agcgcgatca gcccgatcag accacgttcg cttaagacat gcaggtagag
    10261 gctgtgcggc gcgaccttgt cgatgtggaa gtacggatgc ccttcggggt agacgcccag
    10321 gtggacgcgg tgatagttgc ccaggccgac ccccaccagc gggtggatcc ggaacagccc
    10381 gatcgccgac agccagatgt ccatccggtc ctcgtaggcg aatagttcgg tcacacgatg
    10441 gcgaaacacc ggactgaaca gcgccaccga ccccgccagc aggacgatcg tcgtgaccgt
    10501 cagccccacc gccagcgccg ttcggcccca gcgcagccgc tccggcccgt tggtgatcat
    10561 cacggcggcg atcaccaccg ccagaccggc cgtcagggcg atccagcccc cgcgctgctg
    10621 cgtcatcagt tgggtcaggc agagcaacag caccagcgac cagtagaacc agcggacgcg
    10681 aatggccgcc agccggaacg gccgccggct gagcagcgcg gcgcgctcgg ggtgcctcgc
    10741 cacgcgcccg atcaccgcca gcgcgagcgc ccccggcgcc agcgccgcca gatactgcgc
    10801 ataccagccc gaatgccagt agagcgattg cagccggtcg tatccgaacc gcgagatgtc
    10861 ggggttttcc gcgcgccacc aggccagcga cagccagtcg aagtattccc ccacgcccag
    10921 cagcgccgac gccgccaggc tcaccaccag cagccaccac agccgcagca ggcgcagcgc
    10981 gggcaggcgc cggtcgcgca gcgcgacgta gagcccggcg ctcaacgcca gcttcagcgc
    11041 cagttgcagc ccgaacgtgt agctcgtccc gtaatggtta tgaatcacga acaggaattg
    11101 ccgcggatac gtcagcagtt cggcccacag ataacgccac tggggaaaga cgcagacgca
    11161 cgagtagagc acgaagaacc agaccgccgc gtccagccgg ctgcgcttgg gactgaccca
    11221 cagcagcagc cgggtcgtca gatgccccga gaccaggccc atcagcccca tctcgaccag
    11281 gtagaggtga tgcggcccgc ccgggttgtt gccgaaccac ggcagcgcca gcgccagcga
    11341 atagaagtag cccatcggcc acaacgcccc gagcacaaac cagagcagcc cgaggacgcg
    11401 ccccagcgtc ggcgccccca gccagagcca gccgaacatc gcaagccaga acaggagtcc
    11461 aagcccgcag aggagctgat cggccgaagc gcgcgcttcc tgcccgtcgg tcagcgcgcg
    11521 ccaggcacgg cggggttcgg ggacgggggg cgcgctggag gttgcgttca tgaaacggag
    11581 cctgtcgcca gagtgcggcg tgactgctgc gattgaacct gaaaaccgac cgacccgcca
    11641 cgttcgtctg cctggcacgc gcgggaaccg gcgccggctc gccggtgtct atcatcgcac
    11701 gccgcgcttt tgagggtgaa cccgccgcgg cagccggtta ggatgaaccc atcccccggg
    11761 cacacccgga cagatcagga ggccggactc tcatggcacc attttcacgc atcgcgcggg
    11821 cggtcgccct gctggcgctg acgatcgggg tgctcccgct gatgggctgc ctgcgcacga
    11881 cgcataaggt ggaaacctat cacaagatcg acgcgcatat cgtcctcgat gtgcgccaga
    11941 tccgcgacga ggccgaacag gtcgagacct acgtccgcag cgacgatccg gccccccagg
    12001 ccggcggcga ccagccggtc tcgcgcgtgt ggaccgagcc gctccccgat tccgactcgc
    12061 tcttcgcccg cgtcttccgc ttcagccccg tcgcgctggc cgtcacgccc caggaggccg
    12121 acgccgcgaa gaaggcccgc aaggagcgca gcaagcaggt cgacgaagcc ctcaccaaac
    12181 actgggtcgg cgaagacgac cacggctatc tggccgtgcg gctgggcaag gatgaggata
    12241 aggagttgcg cgcgcgcgtc gatgcgctgg tcaaggccga gaaccgcgac cgcaagacga
    12301 tttatcagga aatcgccgaa cgcaagggca acggcaagga actgctcgag gcggtcgaga
    12361 tcatcttcgc cgacgaaatc cgcaacaagc tgaagaaggg ccagctcttc cgcgcgcccc
    12421 gcgacgccaa gggcttcgat gtcttccggg attcgcccct gggccgcaaa taccccgatg
    12481 cccgccccgg cgactggctc gagaagcgat agcgcggcgc cgcgaaccgg cccccgcgtc
    12541 cccgatgttg cgcgcccgcg accaccgcgc gggcgcgcaa ccctcgcttg attttagatc
    12601 ggccgtttgt caccatgcgg cgtctgttct ctgaacggat gacagacgcc cggccgcggc
    12661 cacgccgacg atgcgaccgg ccgggattct ctcaagaaag gttctgccgc catggccaag
    12721 gaagcattca agcgcaccaa gccgcacgtg aacgtgggga cgatc
//
LOCUS       DAOPCO010000091        12587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4973_length_12587_cov_4.578439, whole genome shotgun sequence.
ACCESSION   DAOPCO010000091 DAOPCO010000000
VERSION     DAOPCO010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12587
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4973_length_12587_cov_4.578439"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            529..1587
                     /locus_tag="PLS90_08085"
     CDS             529..1587
                     /locus_tag="PLS90_08085"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPK02404.1"
                     /translation="MRVTQDIHVSRLVPLIAPCDLKAELPMTEPANETVAAAREAIIR
                     ILRGEDRRLIVVVGPCSIHDERAGMEYARRLQGLARELSDRLLIVMRLYFEKPRTTLG
                     WKGLINDPLLDGTFDMETGLRKVRRLLIDVAEMGLPAATEMLDPITPQYTADLISWAS
                     IGARTTESQTHRQMASGLSMPIGYKNATNGDLMAAINAMQAARSPHTFLGIDGAGRTC
                     VVHTTGNPFGHIILRGGHDGPNYDSACVADAVERMSKAGLPPLVMIDCSHANSRKNYL
                     FQARVFHDVLRQRAEGNPAIIGMMVESNLEAGNQPLTGDLASLRYGVSITDACLGWDD
                     TADLLRAAHEALGVSKVA"
     gene            complement(1785..4319)
                     /locus_tag="PLS90_08090"
     CDS             complement(1785..4319)
                     /locus_tag="PLS90_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPK02405.1"
                     /translation="MRLLHRDPSIRAVEIPRPIVPVALAHLSGIVIGLALAAPPGWLA
                     AVSVISLLISARRLWRARRIDGPELGTGWPLLVAVAALGGLQGGALERGDRAATATIA
                     PLVGEKLVEVTGIVAEPPLERPYGVEILLESATLLPLRGGAPAPRSIGGAHRIALLVQ
                     GEAAARLSGRPLPVPGARVKAWGDVLGTRADTRPGTFDSTAYWRGRAVVARLSVDRPG
                     DLLLGETVRPWRSAVHRLLRRVQEGLARGFASRLPARHADLARALVLGEAHRLPPAER
                     EAFGEVGLAHLLAVSGLHTGVVLGLLVVLARLSGLSPRRGALIAIGGLAVYMLLTGLR
                     PPVVRASVMGFFILAGLALGRFTTALASIALACCVTLLIDPRSLLRLDWQLSYACALS
                     IILLAPPLGELFGGPADDDPLLGARRRGSRWRHRLRRWIVLPFAVTLAVQFGLAPIQL
                     FYFHQFNPYAPLVNLPAIPLTGVALIGSLATGLVGPVSVLGAAAGWVGRIALDLLLQL
                     NQAFAQWPGIRITMLPLPAPWLALYYALLLGGSGLLTGEGRRLLLTARQRASLAGCML
                     GAAAVLAWAPGPPRGSGEILDLFILDVGQGDAAVVRFPNGKLMVIDAGRQPPADQGRL
                     VVAPFLRTLGADRIDCLVLTHADADHIGGAPYLLDHFPIEAILEGPDLSDSTTFEDYR
                     ARREPLRDRVIRARWGASLKGFEPVAVQLLGPVPGRNDNDASVVLLIDFDDVEILLPG
                     DLEQAGERAVIEAGLSRDVEVVKLGHHGSRTSTSEALLEAFQPELALISVGRNNRYGH
                     PAPEVLARLREFGVATARTDRDGTLWLRTDGRRIQLWRYAGKRGRP"
     gene            complement(4328..6637)
                     /gene="priA"
                     /locus_tag="PLS90_08095"
     CDS             complement(4328..6637)
                     /gene="priA"
                     /locus_tag="PLS90_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPK02406.1"
                     /translation="MTRAAPPPPGPGRLSYARVAVGRPLPEPLTYYIPEKWSEVIRVG
                     SLVAVPLRSEEAAGFVIDLADKPDIRPQLVRPIARPLSPDYTLEPELIDLGRWIADYY
                     FASLGETLATVSMIGLSDVRPRTRTLLRLAHVEHWLTRNREQAPDGKRATAGHRQVID
                     ALLTAGNRPMAAEDLRAAASVGPGVIKTMLERGWLVASDEIVERDDEYDVDGPPTRSE
                     EIQLTPRQQAVHDELITALHSGAYHAFLLHGVTGSGKTEIYLRVIEEGLRTGRSAIVL
                     VPEISLTPQVVEAFRRRLGGVVGVYHSKLNAGQKFDLWRRIRARETRVVIGARSALFS
                     PLPNLGVVIVDEEHEASYKQDSTPRYHARDMAVLRATRAGAVVILGSATPSIESLHNA
                     REGKYRRLTLPERIGPHASPVMTVVDMKRHVSQGSGELLGGHLISPLLEEAIHERLCR
                     GEQVLLLLNRRGFANQVMCLACSQTLQCPHCDVSLTYHKTLDQLMCHWCGYKQRVPAV
                     CPRCNKAGEIHKLGLGTQRIEETLAEKFPGARSIRIDVDSMRRKGAFQQAWRAITQGE
                     IDIILGTQMIAKGLHLERVTLVGVISADFALFLPDFRAAERTYSLLTQVAGRAGRGQF
                     PGEVILQTFMPHHYAIDAAARLAEEAFYERELHIRRMLRFPPFSRLAGLILSGKDENL
                     VRDQAAQLAGFLKTLAYRPRFRAVRVLGPTPAPIGRIEDQYRWRILIRSAHAGLLHTL
                     LGDGLREFERHHKKSQVNLTLDIDPQDLL"
     gene            complement(6634..9159)
                     /locus_tag="PLS90_08100"
     CDS             complement(6634..9159)
                     /locus_tag="PLS90_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02407.1"
                     /translation="MHDSPGRSAALRPGRGRLPAGSRPWVGPVLLLLLVTLLHERSLP
                     WRGVVPFDERILHLGNGLFSWRCLRAGTLPLWNPYAFCGLPHLATHHAAALYPPVYLF
                     FAALPPFFAFAWMQVFHRFLMGLGTFALLRFGWRLRHGPALAGGVCFMLSGYHIAHEP
                     HQPMIWAAAWAPWMLWCSRETLRGRRWAGPLLAVLLALQIAAGYMQLTVMTLLVLGAE
                     GVWLARPRRGRRFRLGPPVRLAFALAVGIGLMAVQLWMTRDLLPMTFRGTPHFEGFIS
                     HRFDLPRLIAIFFPMVFGAGVTSSFFDEPYFGVWDFPEMIVSLPAPAWLGIGLLLLGP
                     ILVKPGRLAPLRRTVRDGVRFWFVFALFCLLLLPAEVSPLPRLLYHLPVLNLFRIQTR
                     WMALGSLALALLAAAGFDRLLGAVADGRRLRLAPPLILGATALLAAYPAAGLWLMLTL
                     RWGQPEIDWPWFVTEWFRLSNPAIWLPLAFGAGALALLPLAQLARRRGGALMLGWIAL
                     VVVEQMILARHMIFRFDMTQAEATPPRNEVVAALREWHGADPADVRVLPVTGDDIRQT
                     VETLPLFFPQINGVYSLGGNWPLISLDYSLLLRMANIGTLEDPEGLLARPELLAPFNA
                     RYLLFGYRFEGDIYRPYTAGMAPPALLERVFAGDYPWLVPRLTTAKGATIVENTRALP
                     RAWMVERLEPVAGPREVIDRAWREPPDLDPANVALVELMGEQRLGPTDALARGSVAIT
                     ERRADRLRLAVESPGGPGFVVLSETWYPWWWTRVNGELTEVYRVDGVLRGFYVPEGRS
                     EVYMRYLPPAFKQGLAVSLLAGAVWAGWVATALILSRRRKAGP"
     gene            9484..10257
                     /locus_tag="PLS90_08105"
     CDS             9484..10257
                     /locus_tag="PLS90_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02408.1"
                     /translation="MLRRSVTFWILLLIIPSANAQNPQMDFDGCAKFNVTQSAPTNKA
                     VTADARNATNDNYGVYATAYYGTNAYGLYARGYGASGENFAAWLHGRTKVSGLLDVSG
                     WVLPTSTNAFGLGNAGLQWKYIYTEDNIVRGMRDFSSLDLVQAIKASTPPAAKPELSF
                     HSRKHLNVAGGFQELDPASLPVRLEGGGTYYLVSREFSSDQYYDQYPLVGSDVMDALE
                     LTAPNDPVAGYFTWSASGLGSWTSGDSMKSYGPVNMKVE"
     gene            10355..>10820
                     /locus_tag="PLS90_08110"
     CDS             10355..>10820
                     /locus_tag="PLS90_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK02409.1"
                     /translation="MAMATATTSTIGSDPQFADPKLIGGPFYPITLADIDADGDLDLV
                     MSFTDFYQRPKPIVWLENGGFGNPPFADYHTIGEEGSGIVRSIWDSDMDGDGDTDVIV
                     ARYDSNQYSLVIAWYENNGSMPPSFVRHEVCSFAIGIGNIGYGSCTVADMDSD"
     assembly_gap    10821..10830
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10831..11579
                     /locus_tag="PLS90_08115"
     CDS             <10831..11579
                     /locus_tag="PLS90_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK02410.1"
                     /translation="DSHQDILAVYWAKNGDALLESHIGWLRNSGTQPPTFTEQTISSS
                     AAGASFVTAVDLNGDGHMDVVANAPTDSDIVYYQNDGGSPPEFTTITAWSGGAFNLAA
                     DLDGDDDIDLVAGRSWWENNGSNPPEFTLHAVDAIPSDNAPRSLATVDLNNDGLLDIV
                     GSGLNDRTLYWAENEGGTPLQFRVDSIGSVNYATISIATGDVDEDGDQDIVAANWIQP
                     SVQPMGGLFYYRNRLIDKTYVAERAWEIYR"
     gene            11813..12163
                     /locus_tag="PLS90_08120"
     CDS             11813..12163
                     /locus_tag="PLS90_08120"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HPK02411.1"
                     /translation="MYIAVMGSDGLVRYRGIAGVKQLGEATGKIHPSDFNRLAMFLVK
                     AGYMELDSTYSGDALNASGVYTTAVRNGERRVILNYDNRGPEDLWAIEQLIDHTLTKV
                     VWDRPTTSTATIAP"
     gene            complement(12247..>12587)
                     /locus_tag="PLS90_08125"
     CDS             complement(12247..>12587)
                     /locus_tag="PLS90_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPK02412.1"
                     /translation="VRREPDFMQAVPLLRPSGRMVIMAGRDATPAFPVGPFYTRDCKL
                     FGYAMFNATSEKQRECAEDINHWLSERKLRANICKTMKLAEAAEAHRIQEENTIHHAG
                     KLSGKIVLTP"
BASE COUNT         2389 a         4301 c         3948 g         1939 t
ORIGIN      
        1 gagcgacatg acatgcgggg cgcggtcggc ggccgccgcg cgcccatggc cggggttcgg
       61 gaggaggaag aatgcagtgc aacttcggtt gtcggggcga atcgctggga atgagcccgg
      121 ggcaacgggc cgctgaggtt ccccgggcgg ggggactccc tgagtatgaa aaaccctccc
      181 tcccgtgtca acgcccaccg agaaaatcgt ctctcgccct catcaccaga gtccccctca
      241 aaaattcgtc tcccggctcg cgtccctcgt cctcggctct cgatatgttc ccgaaacctc
      301 cttttgatcg tgtccccggc tctcaaaacc gcatcacgca tcccgccagc ccctgcaatt
      361 gttttgcccg actcggccgg ttcgacatag aatctgtctg aaagcacctg ctgccggacc
      421 gcccagcagg ccagtcccgt cggggacggc ggacgccagc ccgccgcttc catgggacaa
      481 tgaacgtatt ctcattgaac caatgcagtc tggcgcgagg gggcattcat gcgggttact
      541 caggatatcc atgtcagtcg gctggtgccg ctgatcgccc cgtgcgatct gaaggccgaa
      601 ctgccgatga ccgagccggc caacgaaacg gtcgcggcgg cgcgcgaggc gatcatccgc
      661 atcctgcgcg gggaggaccg tcggctgatc gtcgtcgtcg gcccctgctc gatccacgat
      721 gagcgcgccg gcatggaata tgcccgccgc ctgcagggcc ttgcacgcga actgagcgac
      781 cgcctgctga tcgtgatgcg gctctatttc gaaaagcccc gcacgacgct cggctggaag
      841 ggcctgatca acgacccctt actcgacggc acgttcgaca tggagaccgg gctgcgcaag
      901 gtccgccgcc tgctgatcga cgtcgccgaa atgggcctcc ccgccgcgac ggaaatgctc
      961 gatccgataa ccccgcagta caccgccgac ctgatctcgt gggcctcgat cggcgcacgc
     1021 accaccgaaa gccagaccca ccgccagatg gccagcggcc tgtcgatgcc gatcggctac
     1081 aagaacgcga ccaacggcga cctgatggcc gcgatcaacg cgatgcaggc cgcccgcagc
     1141 ccgcatactt ttctcggcat cgacggcgcc ggacgcacct gcgtcgtcca caccaccggc
     1201 aatcccttcg gtcacatcat cctgcgcggc gggcacgacg gccccaatta cgactcggct
     1261 tgcgtcgccg acgccgtcga gcggatgagc aaggccggcc tcccgccgct cgtcatgatc
     1321 gattgcagcc acgccaattc acgcaagaac tacctgtttc aggcgcgcgt cttccacgat
     1381 gtcctgcgcc agcgcgccga gggcaacccg gcgatcatcg gcatgatggt cgaaagcaat
     1441 ctggaggccg gcaaccagcc attgacgggc gacctggcca gcctgcgcta cggcgtctcg
     1501 atcaccgacg cgtgcctcgg ctgggacgac accgcggatc tcctccgcgc ggcgcacgaa
     1561 gcccttggcg tcagcaaggt tgcctgatta tgaagatgcg gagcagggcc ttgtcggcct
     1621 cccgaacgaa gacgaagaag cgcactggaa cgacggagaa catccgttcc ggcttaaaaa
     1681 agacccagcg cgcgcggtaa ccaaccagac gctgataaat cggtatcggt atcgccatcg
     1741 ctatcggtct cgaaaccgcg cactacgcga aaccccgccg cgtttcacgg ccggccgcgt
     1801 ttgcccgcgt agcgccagag ctggatccgc cggccgtcgg tccgcagcca gagcgtcccg
     1861 tcgcggtcgg tgcgggccgt cgccactcca aactcgcgca gccgggcgag cacctcgggc
     1921 gccggatgcc cgtaacggtt gttccgcccg acgctgatca gcgccagttc gggctggaag
     1981 gcctcaagca gtgcctcgct cgtggatgtg cggctcccgt gatgccccag cttgacaacc
     2041 tccacgtccc ggctcaaccc cgcctcgatc accgcccgct cgcccgcctg ctccaaatcc
     2101 cccggcagca ggatttccac atcatcgaaa tcgatcagca ggacgaccga ggcgtcgttg
     2161 tcgttccgtc cggggacagg ccccagaagc tggaccgcga ccggctcgaa gcccttaagc
     2221 gatgcgcccc agcgggctcg gatgacccgg tcgcgcagtg gctcgcggcg cgcgcgatag
     2281 tcctcgaaag tcgtggaatc ggaaaggtcg ggaccttcca ggatggcctc gatcggaaag
     2341 tgatccagca ggtagggcgc cccgccgatg tgatcggcat cggcgtgcgt cagcaccagg
     2401 caatcgatcc ggtcggcgcc gagcgtgcgc aggaatggcg cgaccacgag ccgcccctga
     2461 tcggcggggg gctgccgccc ggcgtcgatc accatcagtt taccattcgg gaaacgaacg
     2521 accgcggcgt cgccctgccc gacatcgagg atgaacagat cgagaatctc cccgctcccg
     2581 cggggcggac ccggcgccca cgccagcacc gccgcggccc cgagcatgca gccggccagg
     2641 ctcgcgcgct ggcgcgccgt caacagcagg cgtcgcccct cgcctgtcag cagcccgctg
     2701 ccgcccagca gcaatgcgta gtacagcgcg agccacggcg ccggcagcgg cagcatcgtg
     2761 atgcgaatgc ccggccactg ggcgaacgcc tgattgagct gcagcaggag gtcgagcgcg
     2821 atccgcccga cccagcccgc cgccgccccc agaacggaaa cgggcccgac cagcccggtc
     2881 gcgagcgagc cgatcagcgc gaccccggtc agcggaatcg ccggcaggtt gaccagcggc
     2941 gcgtaggggt tgaactggtg gaaatagaag agctggatcg gcgccaggcc gaactggacg
     3001 gcgagcgtca cggcgaacgg gagcacgatc cagcgccgca ggcgatggcg ccaccgggaa
     3061 ccgcgccggc gcgcgccgag cagcgggtcg tcgtcggccg gcccgccgaa cagctcgccc
     3121 agcggcggcg cgagcagaat gatcgacagc gcgcaggcgt aggagagctg ccagtcgagg
     3181 cgcagcaggc tgcgcggatc aatcagcagc gtgacgcagc aggccagtgc gatcgacgcc
     3241 agcgccgtgg tgaatcggcc cagcgcgagg ccggcgagga tgaaaaatcc catgacggag
     3301 gcgcgcacaa ccggcggccg caacccggtc agcagcatgt aaacggcgag cccgccgatg
     3361 gcgatcagcg cgccgcggcg cggcgacagc ccgctcagcc gcgccagcac gacgagcaac
     3421 cccaggacga cgcccgtgtg gagccccgac accgcgagca gatgcgccag ccccacctcg
     3481 ccgaaggctt cgcgttcggc cggtggcagc cggtgcgcct cgccaaggac cagagcgcgg
     3541 gccaggtcgg cgtggcgcgc cggcaaacgc gaggcgaaac cgcgcgcgag cccctcctga
     3601 acgcgccgca gcagccgatg gacggccgat cgccagggac ggaccgtctc gcccagcagc
     3661 agatcgccgg gccggtcgac gctgaggcgg gccacgacgg cgcgcccgcg ccagtaggcg
     3721 gtcgaatcga aagtacccgg ccgcgtgtcg gcgcgcgtgc cgagcacatc gccccaggcc
     3781 ttgacccgtg cgccggggac gggcagcggc cgaccactca gccgcgcggc cgcctcgccc
     3841 tgaacgagca gtgcgatccg gtgcgcgccg ccgatcgacc gcggcgcggg cgctcccccg
     3901 cgcaacggca gcagcgtcgc gctctcaagc aggatctcca cgccgtacgg ccgctccagg
     3961 ggcggctcgg ccacgatgcc ggtcacctcg accagcttct cgccaaccag cggcgcgata
     4021 gtcgccgtcg cggcgcggtc gccgcgttcc aaggcccccc cttgcagtcc gcccagcgcg
     4081 gccacggcca ccagcagcgg ccagcccgtg cccagctccg gaccatcgat ccggcgcgcg
     4141 cgccagagcc gccgcgctga tatcagcagg ctgatcacgc tcacggccgc gagccagccc
     4201 ggcggcgccg ccagcgccag accgatcacg atccccgaca gatgcgccag cgcgaccggt
     4261 acgatcggcc gcggtatctc gaccgcccga atggatggat cgcggtggag caggcgcatg
     4321 gcggtggcta caacagatcc tgcggatcaa tatccagcgt caggttgacc tgcgatttct
     4381 tgtggtggcg ctcgaactcg cgcaacccgt cgcccagcag tgtgtgcagc aacccggcat
     4441 gcgcgctccg gatcaggatc cgccagcgat actgatcctc gatccgcccg atcggagcgg
     4501 gcgtcgggcc cagaacccgc accgcccgaa agcgcggccg atatgccagc gtcttcagaa
     4561 acccggcaag ctgcgccgcc tggtcgcgca cgaggttctc gtccttgccc gacaggatca
     4621 ggccggccag gcgggaaaag ggcggaaatc gcagcatccg gcggatgtgc agctcgcgct
     4681 cgtaaaatgc ctcctcggcg agccgggcgg cggcatcgat cgcgtagtga tgcggcatga
     4741 aggtctgcag aatgacctcg ccgggaaact ggccccggcc ggcgcggccg gccacctggg
     4801 tcagcagcga gtaggtccgt tccgccgccc ggaaatcggg aagaaacaac gcgaagtcgg
     4861 ccgaaatcac gccgacgagc gtcacccgct ccaggtgcag ccccttggcg atcatctgcg
     4921 tccccaggat gatgtcgatc tccccctgcg tgatcgcgcg ccatgcctgc tgaaacgccc
     4981 ccttgcgccg catcgaatcg acgtcgatcc ggatcgaccg cgcgccggga aatttctccg
     5041 ccagcgtctc ttcgatccgc tgcgtcccca gccccagttt gtggatctcc cccgccttgt
     5101 tgcaccgcgg gcagaccgcc gggacccgct gcttgtagcc gcaccagtgg cacatcagtt
     5161 gatcgagcgt cttgtgatac gtcagcgaca cgtcgcagtg cgggcattgc agcgtctgac
     5221 tgcacgccag gcacatcacc tggttggcga acccccggcg gttgagcagc agcagcacct
     5281 gttcgccccg gcacagccgc tcgtggatcg cttcctccag cagcggcgaa atcagatgcc
     5341 cgcccagcag ttccccgctc ccctggctga cgtggcgctt catatcgacg acggtcatca
     5401 ccggcgaggc gtgcggcccg atgcgctccg gcagcgtcag ccgccggtac ttcccctcgc
     5461 gcgcattgtg cagcgattcg atcgagggcg tggccgaacc gagaattaca acggcgccgg
     5521 cgcgcgtcgc gcgcagcacc gccatgtcgc gcgcgtggta gcgcggcgtc gaatcctgct
     5581 tgtagctcgc ctcgtgctcc tcgtcgacga tcacgacgcc cagatttggc agcggcgaga
     5641 acagcgccga gcgcgccccg atgacgaccc gcgtctcccg cgcgcgaatc cgtcgccaca
     5701 ggtcgaactt ctgccccgcg ttcagtttgc tgtgatagac gccgacgacg ccccccagcc
     5761 ggcggcggaa ggcctccacc acctgcggcg tcagcgaaat ctccggcacc agcacgatcg
     5821 ccgaccgtcc cgtgcgcaac ccctcctcga tcacccgcag gtatatctcc gtcttcccgc
     5881 tgccggtcac cccgtggagc agaaacgcgt ggtaggcgcc gctgtgcagc gccgtgatca
     5941 gctcgtcgtg caccgcctgc tggcgcggcg tcagctggat ctcctccgag cgcgtcggtg
     6001 gaccgtccac gtcgtattca tcgtcgcgtt cgacgatctc atcgctggcg accagccagc
     6061 cgcgctcgag catcgtcttg atcacgccgg gaccgaccga cgcagccgcc cgcagatcct
     6121 cggccgccat cggccggttg cccgccgtca gcagcgcgtc gatcacctgg cgatgccccg
     6181 ccgtcgcgcg cttgccgtcc ggagcctgtt cccggttccg ggtaagccag tgctcgacgt
     6241 gcgccaggcg cagcagcgtg cgcgtgcgcg gccggacatc gctcagcccg atcatcgaga
     6301 cggtggcaag cgtctccccc agcgaggcga aatagtagtc ggcgatccac cgccccagat
     6361 cgatcagctc cggctccagt gtatagtccg gcgacagcgg ccgcgcgatc ggccgcacca
     6421 gttgtggccg gatgtcgggc ttgtcggcca gatcgatgac gaagccggcg gcttcctcgc
     6481 tccgcagcgg aacggccacg agcgacccga cgcggatcac ctcgctccat ttttcgggta
     6541 tgtagtaggt cagcggctcg ggcagcgggc ggccgacggc cacccgggcg tagctcagcc
     6601 ggccgggtcc gggcggcggc ggcgcggcgc gcgtcatggc cccgccttgc gccggcggct
     6661 caggatcagc gccgtggcca cccagccggc ccacacggcg ccggccagca gactgaccgc
     6721 cagcccctgc ttgaacgccg gcggcaggta gcgcatgtag acctcgctcc gtccctcggg
     6781 cacgtagaac ccgcgcagga ccccgtcgac ccggtacacc tcggtcagct caccgttgac
     6841 cctggtccac caccacggat accacgtttc gctcagcacg acgaaccccg gtccgcccgg
     6901 cgactcgacc gccaggcgca accggtcggc gcggcgctcc gtaatggcca cgctcccgcg
     6961 cgccagcgca tcggtcgggc ccagccgctg ctcgcccatc agctccacca gcgccacgtt
     7021 cgccgggtcc aggtccggcg gctcgcgcca cgcccggtcg atcacctcgc gcggaccggc
     7081 caccggctcc agccgctcca ccatccacgc gcgcggcagc gcccgcgtgt tctccacgat
     7141 cgtcgcgccc ttcgccgtcg tcaaccgcgg caccagccac ggatagtctc ccgcgaagac
     7201 ccgctccagc agcgccggcg gcgccatccc cgccgtgtag ggccgataga tgtccccctc
     7261 gaaccggtag ccgaacagca ggtagcgcgc gttgaacggc gccagcagct ccggccgcgc
     7321 cagcagtccc tccggatcct cgagcgtccc gatgttcgcc atccgcagca gcagactgta
     7381 atcgagcgag atcagcggcc agttgccccc cagcgaataa accccgttaa tctgcggaaa
     7441 gaacagcggc agcgtttcaa ccgtctgacg gatgtcgtcg ccggtcaccg gcagcacgcg
     7501 cacgtcggcc ggatcggccc cgtgccactc acgcaatgcg gcgacgactt cattccgcgg
     7561 cggcgtcgcc tcggcctgcg tcatgtcgaa gcggaaaatc atgtgccgcg ccagaatcat
     7621 ctgttcgacc acgaccagcg cgatccagcc gagcatcagc gcccccccgc gccggcgggc
     7681 cagctgtgcg agcggcagca gcgccagcgc gcccgccccg aacgccagcg gcagccagat
     7741 cgccggattg gacagccgga accactcggt cacgaaccac ggccagtcga tctcgggttg
     7801 gccccagcgc agcgtcagca tcagccacag ccccgccgcg ggataggccg ccagcagcgc
     7861 cgtcgccccc aggatcagcg gcggcgcgag ccgcagccgg cgcccgtcgg ccaccgcccc
     7921 cagcagccgg tcgaatcccg cggccgccag caacgccagc gccaggctcc ccagcgccat
     7981 ccaccgcgtc tggatccgga acagattgag caccggcagg tgatacagca gccggggcag
     8041 cggcgatacc tcggccggca gcagcagcag gcagaacagc gcgaagacaa accagaaccg
     8101 gacgccgtcg cgcaccgtcc gccgcagcgg cgccagccga cccggcttga ccaggatcgg
     8161 cccgagcaac agcagcccga tgccgagcca cgccggcgcc ggcagcgaca cgatcatctc
     8221 cggaaaatcc cacacgccga aatacggctc gtcgaaaaac gacgaggtta ccccggcccc
     8281 gaacaccatc ggaaagaaaa tcgcgatgag ccgcggcaga tcgaagcggt gcgaaatgaa
     8341 cccctcaaaa tgcggtgtgc cgcggaacgt catcggcagc aggtcccgcg tcatccacaa
     8401 ctggacggcc atcagcccga tgccgacggc cagcgcgaac gccagccgga cgggcgggcc
     8461 gagccggaac cggcgcccgc gccgcggccg cgccagccac accccctccg cccccagcac
     8521 cagcagcgtc atcaccgtca gttgcatgta gcccgccgcg atctgcagcg ccagcagcac
     8581 ggccagcagc gggcccgccc agcgccgccc gcgcagcgtc tcgcgcgaac accacagcat
     8641 ccacggcgcc cacgccgccg cccagatcat cggctggtgc ggctcgtgcg cgatgtggta
     8701 gccgctcagc atgaagcaga cgccgccggc cagcgccggg ccgtggcgca agcgccagcc
     8761 gaatcgcaac agcgcgaacg tgccaaggcc catcagaaaa cgatgaaaca cctgcatcca
     8821 cgcgaaggcg aagaagggtg ggagcgcggc gaagaacaga taaaccggcg gatagagcgc
     8881 cgcggcgtga tgcgtcgcca ggtgcggcag cccgcagaac gcatacggat tccacagcgg
     8941 cagcgtcccc gcgcgcagac agcgccacga gaacagccca ttgccgaggt gcaggatgcg
     9001 ctcatcgaac gggacgaccc cgcgccacgg cagcgagcgc tcgtgcagca gcgtcacgag
     9061 cagcagcagc agcaccggcc cgacccacgg tcgcgacccg gccggcaggc gtccgcggcc
     9121 ggggcggagg gcggcggagc gtccggggga gtcgtgcatg gcaaaggcag accggggaaa
     9181 gaatcaggca gtgagaagcc acccatggcg cttccgtttg tcgcacaaat gatgcggatt
     9241 gccaagccgg gatctcgtag gtggagaccg cgtccagaag attcgaccag agcccgggga
     9301 gaacggcagg attgaaagtg gatccctcgc gagtccgcgg agattgcagg ggtctatagg
     9361 tgataccgga gtcaactttt gcgttttgtt attgacaatc aattcagtga ccgcaatagt
     9421 cgtgccgctg ggtttcgcac gctcatgcac cgtctatcaa tcaagcaagg agggtgtgcc
     9481 ccaatgcttc ggcgatcagt cactttctgg atcctcttgc tgataattcc ttccgccaat
     9541 gcacagaatc ctcagatgga ttttgatggg tgcgcaaaat tcaatgttac tcagtctgca
     9601 cccaccaaca aagctgtcac ggcagacgca agaaacgcga ccaatgacaa ctatggcgtc
     9661 tacgccacgg cttactatgg aaccaacgcg tacggtctat atgcccgtgg gtatggcgcg
     9721 tccggtgaaa attttgccgc atggttgcac gggcggacaa aggtgagcgg cctgctcgat
     9781 gtctctggtt gggtgttgcc gacatcaaca aatgccttcg ggctcggcaa cgcggggctg
     9841 cagtggaaat acatctatac cgaagacaat atcgtgcgcg ggatgaggga tttctccagt
     9901 ctcgatttgg tccaggcaat caaagcctct accccgcctg cagccaaacc cgaattatcc
     9961 tttcactcaa ggaagcatct caatgtggcc ggcggttttc aagagctgga cccggcttcg
    10021 ctgccggtac ggcttgaagg aggcggaacc tactatcttg tcagtcgcga gttcagctcc
    10081 gatcaatact acgatcagta tccattggtt ggcagcgatg tgatggatgc gcttgaacta
    10141 actgcgccga atgatccagt tgcgggatat tttacctgga gtgcgagcgg actggggagt
    10201 tggacatccg gtgattcaat gaagtcctat ggtcccgtga atatgaaagt cgagtagtcc
    10261 aatgaagaac atgccagaga tttccgcagc gcatggagag agcggctcaa ggcggaagac
    10321 gggagtgggt gctttcagaa gtgtaattct ctgcatggcc atggccaccg cgacgacctc
    10381 caccattggt tccgatccgc aattcgccga tcccaagctc atcggtgggc ccttttatcc
    10441 gattaccctt gcggatatcg acgccgatgg cgatcttgac cttgtgatgt cgtttaccga
    10501 tttttatcag cggcccaaac cgatcgtgtg gcttgagaac ggtggatttg gtaacccgcc
    10561 tttcgcggat tatcatacaa ttggcgagga gggatcaggt atagtccgat cgatctggga
    10621 ttcggacatg gatggagacg gggatacaga tgtgattgtt gcacggtatg attcgaacca
    10681 atattcgttg gtcattgcct ggtatgagaa caacggatcg atgccaccca gttttgtgcg
    10741 acacgaagtg tgcagctttg caatcgggat cgggaacatc ggttacggtt cctgcacggt
    10801 tgcggacatg gatagcgata nnnnnnnnnn gcgatagcca tcaggacatc cttgctgtct
    10861 actgggcaaa gaacggcgat gctcttttgg agagccatat tggctggttg cgcaatagcg
    10921 ggacacagcc gcctacattc acggagcaaa caatatcctc atcggcggca ggcgcctctt
    10981 tcgtcacggc cgtggatctg aatggggatg gccatatgga tgtggtggcc aatgcgccga
    11041 ctgacagcga tattgtgtac tatcagaatg acggtggttc acctcccgaa tttaccacga
    11101 taactgcctg gagcggaggc gcatttaacc tggccgcgga cttggatgga gatgacgata
    11161 ttgacttggt tgcaggacgg tcgtggtggg agaataatgg cagcaatccg cccgaattca
    11221 cacttcatgc agtcgacgcg attccatcag ataacgcgcc caggagtctg gccacagtgg
    11281 atctgaacaa tgacggactg ctagatatcg ttgggtccgg actcaatgat agaacactgt
    11341 attgggcaga gaatgagggc gggacgccgc tgcaattcag ggtagactcc attggaagcg
    11401 tcaattatgc caccatcagt attgccacgg gtgatgtcga cgaagatggc gaccaagata
    11461 ttgttgcggc gaattggatt caaccttcgg tccagcccat gggcggcctc ttctattaca
    11521 gaaataggct catagacaag acttatgtgg ctgaaagagc ctgggaaatc tacagatagg
    11581 cgaaatgtaa cgaggagata tgttcatgaa ttataggttt tgtcttgcac ttgcatgcat
    11641 ctgttctgtg gctattgccg gtgaaatcgc ggagcatcac ggcaagctgc aggtatcaga
    11701 gcgaggacct tcgcccgact cccgcagcag atccgagctg gcgcccgtgg aacatggggt
    11761 cacggaaatc ggcattgagc gtgcaggtca gccaggaaaa ttcctacacc cgatgtatat
    11821 tgccgttatg gggagcgatg gcttggtgcg atatcgaggg atagcaggtg tcaagcaact
    11881 cggagaggcc acgggaaaga ttcacccatc cgacttcaat cggctggcca tgttcctggt
    11941 gaaggccggc tacatggagc tggacagcac gtattctggc gacgcgctca atgcctcggg
    12001 ggtgtataca accgccgtca ggaacggcga gcggcgcgtg atcctgaact atgacaaccg
    12061 cggcccggag gacctgtggg cgatcgagca actgatcgat cacacgctca ccaaggtggt
    12121 gtgggaccgc ccaacaacgt ccactgccac gatcgcgccg tagcgccaac aggggaacac
    12181 gggcaagatg ccccatgcca cctgtcgtcc gtcaacacag ggattctgcg gctcgcggga
    12241 caccgcttac ggcgtcagca cgatcttgcc cgagagtttg ccggcgtggt ggattgtgtt
    12301 ttcttcctgg atccggtgcg cctcggcggc ttcggccagc ttcatcgtct tgcagatgtt
    12361 cgcccgcagc ttccgttccg acagccagtg gttgatatcc tcggcgcatt cgcgctgctt
    12421 ctcgctcgtc gcgttgaaca ttgcatagcc gaacagcttg cagtcgcgcg tgtagaacgg
    12481 accgacggga aacgccggcg tggcgtcgcg ccccgccatg atcaccatcc gcccgctcgg
    12541 ccgcaacaat gggaccgcct gcatgaaatc gggctcgcgc cgcacgt
//
LOCUS       DAOPCO010000092        12558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_4981_length_12558_cov_4.784132, whole genome shotgun sequence.
ACCESSION   DAOPCO010000092 DAOPCO010000000
VERSION     DAOPCO010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12558
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4981_length_12558_cov_4.784132"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..548
                     /locus_tag="PLS90_08130"
     CDS             345..548
                     /locus_tag="PLS90_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02413.1"
                     /translation="MIRQCCKCRRIWKEGRWLYPRLTELTHRDISHCYCDACFKEEMA
                     TLRAHRRPGPAVAVIRSLRRLFH"
     gene            complement(646..1896)
                     /locus_tag="PLS90_08135"
     CDS             complement(646..1896)
                     /locus_tag="PLS90_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02414.1"
                     /translation="MHDEAAPTPIQIPWQFLGLISFLALSMALAQVLYMRKQIDEELE
                     RAAASMSSSPRMAYQDFHHVLEGSDLLTLGRISEARNQFIDRLDEAVKRPLRLRPAPD
                     SAYSVAREAIDILQELEREYGGDTEITRNLIVIAVKESAQSQLEEGTLSGWQGLQSFF
                     RALEAKGLVPRHLIENYDEWIANIRNVERRPLGLRDAIHRVAQCLQLAFNQLPPLSPA
                     SAGQPPVAPLPFPLSDDGLILADRHLREGLIALRQFDYDFPDPPTPEPILLQLRGQIL
                     FNRASMKLAHVRERGRTPLQLGTDFITDSILRPPGAQLAEVPPPHRMYELFLSHTRAE
                     FEEAIRAFDAAVGLDESLRQAYLALSYAELAMLDELEQIDPGTNRALSEERIAQVTGG
                     VGRRVIDQLRQRNQPLMIVRMPWD"
     gene            complement(1900..2466)
                     /gene="rsmD"
                     /locus_tag="PLS90_08140"
     CDS             complement(1900..2466)
                     /gene="rsmD"
                     /locus_tag="PLS90_08140"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPK02415.1"
                     /translation="MSTRIIAGQRRGMIIRTPRGRQTRPTLGRVRESLFMILAPRLEG
                     ARVLDLFAGSGALGLEALSRGAACCVFVDQAHGAIAALRANVAQLGWRDRAEIVAHNA
                     LRWLAAPPRRPFDLILLDPPYGEETAGRALRRLADALDAWLAPDGVVVAQIGRRDPLD
                     EAFGALRAFRWESYSETRIGFFRRAEEA"
     gene            complement(2463..4016)
                     /locus_tag="PLS90_08145"
     CDS             complement(2463..4016)
                     /locus_tag="PLS90_08145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02416.1"
                     /translation="MKPAPIIFLVVLGAYALTAGGHLYSPDEEILYRMTRALATGPSL
                     AIEPLGDFATRQGRDGRYYGQYGIGQPLLAVPFYWAGRAVAGLAGRSQWNRLYGPSGT
                     APHVVGEEPTAAAYATRWALSWFNILLGALLAVLLYGVCVETTGQREAAGAAALLYAL
                     GSLAWPHSRPFFTESCAAFFILLAWWALLRARRGRVVRWCAVAGAAAGYAALVRNDSV
                     LAYPGLAILLLGPAAWGARAEGRPAWQAWAAFCAPALVCGVILLGMNVVLYGGPFESG
                     YADQPEGLAFTTPVLAGLYGLLFSIGKGLFFFSPALALGFLGWRPLALAQTDQRRLML
                     WALAFVIAVPLVVHAKWQNWSGGWCWGPRHIFLIQPFLAIPIAAWLARPWGPPTRVIL
                     LVALIVGGTVQLLGASQDFIRFYRDHFRRSPHTFFVVYDDYDRMYWESFFQLYSRPEP
                     DPRIPLQPVALYPPAPTQDSLYFPQRSVWAAYPRMIREEGIDNFWVRLAGLGARPRAS
                     DAPETRIQP"
     gene            complement(4013..5086)
                     /locus_tag="PLS90_08150"
     CDS             complement(4013..5086)
                     /locus_tag="PLS90_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPK02417.1"
                     /translation="MSGTRQLMIGAFVVGGLVLFTMVTAMLNEWDFLSQRHIYYGMFK
                     NARELNVGAPILVYGYPSGTVASIRLVPGEYPLMVEMKIDKNIELHADAKVHIISASL
                     IGDTTINIDAGTEEFDVLPPGSHIVGTSGNELEEVAAEVADELISTLRGINNYLYDRQ
                     TQDDFKRIVGNLATATEKMDQTLSIVNEEFRPMVEAMMDSATKWNALVTDARSLVQRA
                     DAQVSVISSQVEGTAAAWESAAGSFSTELEETSAAIQELSASLNQTAGRLDEQIEMNA
                     GRIEQTLEELKTVARSIREMVDQVQSGQGTLGKLITDSGPFDRLNQLLTALSNTLTGS
                     RAPMFNLGEGGSAGTAPGRPTAP"
     gene            complement(5672..6439)
                     /gene="tsaB"
                     /locus_tag="PLS90_08155"
     CDS             complement(5672..6439)
                     /gene="tsaB"
                     /locus_tag="PLS90_08155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPK02418.1"
                     /translation="MPPATQSPRVVLGIESSTPAGGVALADTDGRLLAHLWRESRQPV
                     SGRLLSDIDTLLEGYDLTREAVAAVAVTLGPGAFTGLRVGLAVAKTLAHGLSAPLFAF
                     STLEAAAWRTGLAGEAVAVLLDARRAEVYSGVYRLPEAPSGAMEVLREARAEPLASFL
                     EAIGRTDWPTLHLTGQGADLYREALASAAGVGERLRFVPPPLDRPGADSVARLGAHEL
                     AAGRAGRDPLTLGPVYLRVSDAEKRHRIAANLEWTAR"
     gene            complement(6439..6813)
                     /locus_tag="PLS90_08160"
     CDS             complement(6439..6813)
                     /locus_tag="PLS90_08160"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HPK02419.1"
                     /translation="MRTEQENLAASLCHLFNILPLWGLLIAGAIWFQLREESRRVVLH
                     AQQAMIFNVMGLALVLIWLLVGLLAKVVKYVSLPLGQGLVLVNNGILLVLGAAYVIIC
                     LMGFARCLGGQSFHYPLIKVER"
     gene            complement(6924..7859)
                     /locus_tag="PLS90_08165"
     CDS             complement(6924..7859)
                     /locus_tag="PLS90_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02420.1"
                     /translation="MNRLSFLHLVSMFLIFLAALAWPAVPVQAQEPEGSGEDAPSTQT
                     IQIKLQPVLKETPQRVKLAPVRKGERTQSGAAEPPASSGRATPPRREAQPAEPAADIV
                     RVGPGAPAADDVASALAAPPSSAALDNLRRFVIEQKDSPQIPAVLLRIGQISQVLGDM
                     DEAEMAYQAAAIVAESPEIVYSATLGLAEAAAVRGDFAAAAEHWETLRFEFPDRPAPI
                     AAGAANALALTGSGKFDAGGAAWRELAEVASGDPTAAATLAAARLGEALNAELAGRAD
                     EARALYEQVVAAAADSAEARIARGRLEDLARPLVP"
     gene            complement(7994..8806)
                     /gene="trpA"
                     /locus_tag="PLS90_08170"
     CDS             complement(7994..8806)
                     /gene="trpA"
                     /locus_tag="PLS90_08170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPK02421.1"
                     /translation="MNRIDRMFAARRREGRAAMIFFLTAGCPDLESTEQAIDALAEAG
                     VDLIELGIPFSDPIADGPTIQRASQVALERGTTVKAILDLVRRVRARHPDLALLLFTA
                     YNPVFHYGEAAFVAAAAEAGADGLLIPDLPPEEAGELRRLASERELCVVFLVAPTSSS
                     ARARAICEASTGFVYYISLRGVTGARAELPADIEERLRELKTLTGKPVAVGFGISGPD
                     QARALVPLCDGIIVGSALVRLIGEQPRPTLREDVRRFAGEMCAALAEAATAR"
     gene            complement(8803..10035)
                     /gene="trpB"
                     /locus_tag="PLS90_08175"
     CDS             complement(8803..10035)
                     /gene="trpB"
                     /locus_tag="PLS90_08175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPK02422.1"
                     /translation="MSSPSLPAPPALNDLPDKTGHFGEFGGRYVPETLMGALLDLERE
                     YLAIREDAAFHAELNGLLADYVGRPTPLYEARRLAEELGGKARIFLKREDLAHTGAHK
                     INNTLGQALLARRLGKRRIIAETGAGQHGVAAATACALLGLECAVYMGEEDMRRQELN
                     VFRMRLLGAEVVPVLSGSRTLKDATNEAMRVWMQTVDDTHYIIGSCVGPHPFPMMVRD
                     FQSVIGREARAQCLEKTDRLPDAVLACVGGGSNSLGLFAPFYDDRDVRMFGVEAEGEG
                     ILTGRHACSITTRRIGVFHGSKSYVLQNEDGQITPAHSISAGLDYPGVGPEHSFYAAS
                     GRADYLSVKDDEALAATRQLCRLEGILPALESAHALAALPRVLAQLPAGSTIIVSLSG
                     RGDKDVYTLRGVMQEGEG"
     gene            complement(10384..11328)
                     /gene="hflC"
                     /locus_tag="PLS90_08180"
                     /pseudo
     CDS             complement(10384..11328)
                     /gene="hflC"
                     /locus_tag="PLS90_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
     gene            complement(11325..12317)
                     /gene="hflK"
                     /locus_tag="PLS90_08185"
     CDS             complement(11325..12317)
                     /gene="hflK"
                     /locus_tag="PLS90_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HPK02423.1"
                     /translation="MARVVRINRIVLPKGVGRLIAGLFGAIALIIVVMSSIYTVQAEE
                     LGVVLRFGKFVRMTEPGLRFKLPLGIERVELVPVNRQMKEEFGFRTAHVDVRTDYRPG
                     NYADESLMVTGDRNTVVVEWIVQYRINEPKAFLFNVRNPVDTLRYASEAVMREVVGDR
                     TVDEVLTIGREEVQRVAQDRLQELLNDYQMGLGIEQVALQDVNPPGPVQDSFNEVNQA
                     QQERERMINEAWAEYNREVPRAHGEADQLISEARGYATQRVNEAEGDAARFTALFREY
                     VEAPEVTRQRIFLETMGRLLPRLGRKIILDDAAGQILPLLQLDSPPARVEGGAQ"
BASE COUNT         2130 a         4427 c         3978 g         2023 t
ORIGIN      
        1 gctcctcggg gacgagcttt tttgacgcag ccgctacggc ttcggaatcg cagttgtcga
       61 ctcgctcttt tccactgatt tcgatcgcga tccccccgat tttttctttc accggccctc
      121 cagatcccga gatcgcatct ctcattttga tggaacggtc atcgattgtg agatttcctg
      181 agcacaatgt gtgattttat gccgaccggc taaactccag ataactacca taacatcagt
      241 gacttggatg gctgattggg gatccttgac gattggcatt gaaaatgctc caccctctgg
      301 tcagggaaag cccggggatc caccgctcgg aaggaaccac ccagatgatc cgccagtgct
      361 gcaagtgtcg ccgaatctgg aaggaaggcc gctggctcta tccgcgcctg accgaactga
      421 cccaccgcga catctcccac tgttattgcg acgcctgctt caaggaggaa atggcgacgc
      481 tgcgtgctca ccgtcggccg ggcccggccg ttgcggtgat ccgcagtctt cgccgcctgt
      541 ttcactaacc tccgccgccg ccagaagaac tgagaccaaa cgcccccacg cgtccctcct
      601 gttcctcgct ctcgaactcg gcccacgcat cttgctctgc ctccgctaat cccacggcat
      661 ccggacaatc atcagcggct ggttgcgctg gcgcaactgg tcgatgaccc gtcggccgac
      721 cccgcccgtg acctgcgcga tccgttcctc gctcagcgcg cgattcgtcc ccgggtcaat
      781 ttgctccagt tcgtccagca tcgccagctc ggcatagctc agcgccagat aggcctgacg
      841 cagcgattca tcaagtccga cggcggcgtc gaacgcgcgg atcgcttcct caaactcggc
      901 gcgcgtgtgc gacagaaaca gttcgtacat tcggtgaggc ggcgggacct cggcaagctg
      961 cgccccggga ggccgcagga tcgaatcggt gatgaaatcg gtgccgagct gcagcggcgt
     1021 ccggccgcgt tcgcgcacgt gagccagttt catcgaggcc cgattgaaca ggatctggcc
     1081 gcgcagctgg agcaggatcg gctcgggcgt cggcggatcg ggaaagtcgt aatcgaactg
     1141 ccgcagggcg atcaagccct cgcgcaaatg gcggtcggcc aggatcagcc cgtcgtcgga
     1201 caacggaaac ggcaacggcg cgaccggcgg ctggcccgcc gaggccgggc tcagcggcgg
     1261 caactgattg aacgccaatt gcaggcattg cgccacgcgg tggatcgcgt cgcgcagacc
     1321 cagcggccgc cgctcgacgt tccggatgtt cgcaatccac tcgtcatagt tctcgataag
     1381 atgccgcggc acgagtccct tggcctccag cgcgcggaag aagctctgca agccctgcca
     1441 gccggaaagc gttccctcct cgagctggct ttgcgccgat tccttgaccg caatcacgat
     1501 caaattgcgc gtgatttcgg tgtcgccgcc gtattcgcgc tccagctcct gcaggatgtc
     1561 gatcgcttcg cgggcgacac tgtaggccga atcgggggcg gggcgcagcc gcagcggtcg
     1621 cttgaccgcc tcgtccagcc ggtcgatgaa ctgattgcgc gcctcgctga tccggcccag
     1681 cgtcaacaga tcgcttccct ccaggacatg atggaaatcc tgatacgcca tgcgcgggct
     1741 gctgctcata ctggcggcgg cccgctccag ttcttcgtcg atctgtttgc gcatgtagag
     1801 gacctgcgcc agggccattg acagggcgag gaaactgatc aggccgagga attgccacgg
     1861 gatctggatc ggcgtggggg cggcttcgtc gtgcatgcgt tacgcctcct cggcacggcg
     1921 aaaaaagccg atccgcgtct cggagtaact ttcccagcgg aacgcgcgca acgcgccgaa
     1981 tgcctcgtcg agcggatcgc ggcgcccgat ctgggcgacg accacgccgt ccggcgcgag
     2041 ccaggcatcc agcgcgtcgg ctaatcgccg cagggcgcgg ccggcggttt cctcgccgta
     2101 gggcggatcg agcagaatca aatcgaacgg gcggcgcggc ggcgcggcga gccagcgcag
     2161 ggcattatgc gcgacgattt ccgcgcggtc gcgccagccc agttgggcga cgttggcgcg
     2221 cagcgcggcg atcgccccat gcgcctgatc gacgaacacg caacaggccg cgccgcggct
     2281 cagggcctcc agcccgagcg cgccgctgcc ggcgaacagg tccagcacgc gcgctccctc
     2341 cagccgcggc gccaggatca tgaacagcga ttcgcgcacg cgacccagcg tcgggcgggt
     2401 ctgccgcccc cgcggcgtgc ggatgatcat gccgcggcgc tgaccggcga tgatgcgcgt
     2461 gctcatggtt ggattcgcgt ctcgggggca tcggacgcgc gcgggcgggc gcccagcccg
     2521 gccagccgca cccagaaatt gtcgatcccc tcctcccgga tcatgcgcgg atacgccgcc
     2581 cagacgctcc gctgcggaaa gtagagcgaa tcctgcgtcg gggcgggggg ataaagcgcc
     2641 acgggctgca gcggaatccg gggatcgggc tcgggccggg agtagagctg gaagaacgac
     2701 tcccagtaca tccggtcgta atcatcataa acgacgaaga acgtgtgggg cgagcggcgg
     2761 aaatgatcgc gatagaagcg gatgaaatcc tgtgaggcgc ccagaagctg caccgtcccg
     2821 ccgacgatca gagcgaccag caggatcacc cgcgtcgggg gaccccacgg ccgcgccagc
     2881 cacgcggcga tcggaatcgc caggaagggc tgaatcagga agatgtggcg cggcccccag
     2941 caccaccccc ccgaccagtt ctgccacttg gcgtggacaa ccagcgggac ggcgatgaca
     3001 aaggcgagcg cccagagcat cagccggcgc tggtcggtct gcgccagcgc cagcggacgc
     3061 cagccaagga aacccagcgc cagcgccggc gagaagaaaa acagcccctt gccaatgctg
     3121 aacagaagcc cgtacaagcc cgccaggacg ggcgtggtga aggccagccc ctcgggctga
     3181 tcggcatagc ccgattcgaa cggcccgccg tagagcacga cgttcatccc cagcaggatg
     3241 acgccgcaaa ccagcgcggg agcgcagaac gccgcccacg cctgccaggc gggccggccc
     3301 tccgcgcggg cgccccacgc cgcgggaccc agcaggagga ttgcaagccc ggggtaggcc
     3361 agcaccgaat cgttgcgcac gagcgcggcg tagccggcgg cggccccggc gacggcgcac
     3421 caccgcacga cccgcccgcg gcgggcgcgc agcaacgccc accacgccag cagaatgaaa
     3481 aacgcggcac agctctccgt aaagaacggg cggctgtgcg gccaggccag cgaccccagc
     3541 gcatacagca gcgccgcggc gcccgccgcc tcccgctggc cggtcgtctc gacgcaaaca
     3601 ccgtagagca gcaccgcgag cagcgcgccc aggaggatat tgaaccagga cagcgcccag
     3661 cgcgtggcgt aggcggcggc ggtcggttcc tcgccgacga cgtgcggcgc cgtgccgctc
     3721 ggcccgtaga gccggttcca ctggctgcgc ccggccaggc cggccaccgc gcggccggcc
     3781 cagtagaacg gaacggcaag cagcggctgg ccgatcccgt attgtccgta ataccgtccg
     3841 tcgcggccct ggcgcgtggc gaaatccccc agcggttcga tagccagtga ggggccggtc
     3901 gccagtgcgc gcgtcatccg atagagaatc tcctcgtcgg gcgagtacag atgcccgccg
     3961 gccgtcaacg cgtacgcccc cagcacaacg aggaagatga tcggcgcggg cttcacggcg
     4021 cggtcggccg cccgggcgct gtccccgcgc ttcccccctc gcccaggttg aacatcggcg
     4081 cccggctgcc ggtcagcgtg ttcgacagcg ccgtcagcaa ctggttgagg cgatcgaacg
     4141 gccccgaatc ggtgatcagc ttgcccagcg tcccctggcc ggattgcacc tggtcgacca
     4201 tctcgcggat cgagcgcgcg acggtcttca gctcctcgag cgtctgctcg atccgcccgg
     4261 cattcatctc gatctgttca tcgaggcgcc cggccgtctg gttcaggctg gcggagagct
     4321 cctgaatggc cgcgctcgtc tcctcgagtt cggtgctgaa cgagccggcg gccgattccc
     4381 acgccgcggc ggtcccttcc acctggctgc tgatcacgct gacctgcgcg tcggcccgct
     4441 ggaccagcga acgggcatcg gtgaccagcg cgttccactt cgtggcgctg tccatcatcg
     4501 cttcgaccat cggccggaat tcctcgttca caatcgacag cgtctgatcc atcttctcgg
     4561 tggcggtggc caggttgccg acgatccgct tgaaatcgtc ctgcgtctgc cggtcgtaga
     4621 ggtaattgtt gatcccgcgc agcgtgctga tcagctcgtc ggccacttcg gccgccactt
     4681 cctccagttc attgcccgag gtgcccacga tgtgactgcc cggcggcagc acgtcgaact
     4741 cctccgtccc ggcgtcgatg ttgatcgtcg tgtcgccaat caggctcgcg ctgatgatgt
     4801 gaaccttggc gtcggcgtgc agctcgatgt tcttgtcgat cttcatctcg accatcagcg
     4861 gatactcgcc cggcaccagc cggatgctgg caaccgtccc ggaggggtag ccgtaaacca
     4921 ggatcggcgc gccgacattc agttcgcgcg cgttcttgaa catcccgtag taaatgtgcc
     4981 gctggctgag gaagtcccac tcgttgagca tggcggtgac catcgtgaac aggaccaggc
     5041 cgcctaccac gaaagccccg atcatcaact ggcgggtgcc gctcatcttt gactcccaat
     5101 gcgggcgggt ggaatgtcgc gggcgggccc caccgccctc gactccggcg gcgggccggt
     5161 tgccgacagt ctaccagact tgccagcccc ccgccccgtt tgtctcacgg gattaatgtc
     5221 gtccttcgtc ctcgtcctcg cccccaacag cggccgagtc ggatctcttc gacgccatgg
     5281 agtcggttcg tgccggcgcg ctccgatgcg ccgcccgaag ctgggcatcc cgatcgtgac
     5341 gaacgcctcg cggctgaaaa cttgacgccc tccaaaagct ggcgaatact gaccgtggag
     5401 cttgcgggta accgtctgcc caactccgcc tcccgaggcg gtgcggcaac ggccgtgatc
     5461 cccgcaagct ctcttttgtc ggctacgaga tggtcgtcga agcgacgtct ctggggcggc
     5521 ttccgaacca ccagccgcgg ccgccgcagc ttctcgcctt cgcgtgccac cagatcatct
     5581 cccttttcgt gcgcgttcgt gcctttcgtg atccacatcg ccttctgcgt aatatcctcc
     5641 cctgcctctc tgccgcatcc atcgccatcc cctaacgcgc ggtccattcc agattggcgg
     5701 cgatccggtg gcgcttctcg gcatcgctga cacgcaggta gaccggtccc agcgtcagcg
     5761 gatcgcggcc ggcccggccc gcggccagct catgcgcgcc gagccgggcg acgctgtcgg
     5821 cgccggggcg gtccagcggc ggcgggacga agcgcagccg ctcgccgacg ccggcggccg
     5881 aagccagcgc ctcgcgatac agatcggcgc cctggcccgt cagatggagc gtcggccagt
     5941 ccgtccggcc gatcgcctcc agaaagctgg cgagcggttc ggcgcgcgcc tcgcgcagaa
     6001 cctccatcgc gcccgacggc gcctccggca accgatagac gccgctataa acttccgcac
     6061 gccgggcgtc aagcagcacg gcaacggcct ctccggcgag accggtgcgc caggccgccg
     6121 cctccagcgt ggaaaacgcg aacagcggcg cggacaatcc gtgcgccagc gttttggcca
     6181 cggccaggcc cacgcgcagc ccggtaaatg cccccggccc gagcgtcacc gctaccgccg
     6241 cgaccgcctc gcgcgtcaga tcgtatcctt ccagcagcgt gtcgatgtcg ctcagcaggc
     6301 gtcccgagac cggctggcgg ctctcgcgcc acaagtgggc cagcagccgc ccgtcggtgt
     6361 cggccagcgc cacgcctccc gccggcgtcg aggattcaat cccgagcacc acccgcggcg
     6421 attgcgtggc cggcggcact accgctccac cttaatcagc ggataatgga acgattgccc
     6481 ccccaggcaa cgcgcgaacc ccatcaggca gatgatgacg taggccgccc ccagcaccag
     6541 caggatgccg ttattgacca ggaccagccc ctgccccagc ggcagcgaga catacttgac
     6601 gaccttcgcc agcaggccca cgagcagcca gatcaggacg agcgcaaggc ccatcacatt
     6661 gaaaatcatc gcctgctgcg cgtgcaggac gacgcggcgg ctttcctcgc gcaattgaaa
     6721 ccagatggcg ccggcgatca acaatcccca caacggaagg atgttgaaca ggtgacagag
     6781 tgaagcggcg aggttttcct gttccgtgcg catggaagga tgtcggactt gggatcgagc
     6841 ggccccggga agaacgaccg ccgggcggca accggttcaa tttgatccgt cgccgccggc
     6901 caggtcaagc cggatgcggc cgctcacggc acgagcggac gggccagatc ttccaggcgg
     6961 ccgcgggcga tccgcgcctc ggccgaatcc gcggccgcgg cgacgacctg ctcgtaaagc
     7021 gcgcgggctt cgtccgcccg cccggcgagt tcggcattca gggcttcgcc cagccgtgcc
     7081 gcggccagcg tcgccgccgc cgtcggatcg cccgaggcga cctcggccag ttcacgccag
     7141 gcggcgcccc ccgcgtcgaa cttcccactg ccggtcagtg ccagcgcgtt cgcggcgccg
     7201 gcggcaatcg gcgccggccg gtcgggaaac tcaaagcgga gcgtctccca gtgttcggcg
     7261 gcggcggcga aatcgccgcg aacggcggcc gcttcggcca gccccagcgt agcggaataa
     7321 acaatttcgg gcgactcggc gacgatcgcc gcggcctgat atgccatctc ggcctcgtcc
     7381 atatcaccca acacctggct gatctggccg atccgcagca gcacggcggg aatttgcggc
     7441 gagtccttct gctcaatgac gaagcgtcgc aggttgtcga gcgccgccga cgaaggcggg
     7501 gccgcgagcg cggaagcaac atcgtcggcc gccggcgcgc ccggaccaac gcggacgata
     7561 tcggccgcgg gttcggcggg ttgggcctcc cgccggggcg gcgtcgcccg tccactggac
     7621 gcgggcggtt cggccgcgcc gctctgagtc ctctcgccct tgcgcaccgg cgcgagcttg
     7681 acccgctggg gggtctcctt caagaccggc tgcagtttga tctgaatggt ctgcgtcgaa
     7741 ggcgcgtcct caccggagcc ttcgggctcc tgggcctgca ccggcactgc gggccacgcc
     7801 agcgccgcga ggaagatgag gaacatcgaa acgaggtgaa gaaaactcag ccggttcatg
     7861 gttcagggtc tggccgttgc ggcggcccag ctctcagtcg caagttgact gaatcttgtc
     7921 gcatcaccgc gcaatgcgcc agtgaaacgt ggcgggaaga aaaggggacg gaggggatca
     7981 caacaccggc cattcaccgg gcggtcgcgg cctcggcaag cgccgcgcac atctcgccgg
     8041 cgaaccggcg aacgtcctcc ctcagcgtcg gccgcggctg ctccccgatc agccgaacca
     8101 gcgcgctgcc gacgataatg ccgtcgcaca gcgggaccag cgcgcgcgcc tgatcaggtc
     8161 ccgagatgcc gaacccgacg gcgaccggtt ttccggtcag agtcttcagc tcacgcagcc
     8221 gttcttcaat gtcggcgggg agttccgccc gggcgcccgt gaccccccgc agcgaaatat
     8281 aatagacgaa gcccgtcgac gcctcgcaga tggcccgcgc ccgcgcggag gaggaagtgg
     8341 gcgccaccag gaacacaacg cacagttcgc gctcgctggc cagccgccgc agctcgccgg
     8401 cctcctcggg cggcagatcg ggaatcagca agccatcggc cccggcctcg gccgccgccg
     8461 cgacgaacgc cgcctcgccg taatgaaaaa cagggttgta ggcggtgaac aacagcaagg
     8521 ccagatcggg gtgccgggcg cgcacacgcc gcaccagatc cagaatcgcc ttgacggtcg
     8581 tcccccgttc cagcgccacc tgcgaggcgc gctgaatcgt cggcccatcc gcgatcggat
     8641 cggaaaacgg aatcccgagc tcgatcagat cgacgccggc ctcggccagc gcatcgatcg
     8701 cctgctcggt cgattccagg tccggacagc cggccgtcag gaagaagatc atcgcggcac
     8761 ggccttcgcg ccgccgcgct gcaaacatcc ggtcgatccg attcatccct cgccctcctg
     8821 catcacgccg cgcagtgtat aaacgtcctt gtcaccgcgc cccgacaggc tgacgatgat
     8881 ggttgatccg gccggcaatt gcgcgagcac cctgggcagc gcggccagcg cgtgcgccga
     8941 ttcgagcgcc ggcagaatcc cctccagccg gcagagctgg cgggtggcgg ccagcgcctc
     9001 gtcgtccttt acgctcaggt agtcggcgcg gcccgacgcg gcatagaagc tatgttcggg
     9061 cccgacgccg gggtaatcca ggccggccga gatgctgtgc gccggcgtga tctggccgtc
     9121 ctcgttctgc agcacgtagc tcttcgaacc gtgaaagacg ccgatccgcc gcgtggtgat
     9181 cgagcacgca tggcgtccgg tcagtatccc ctcgccctcg gcctccactc cgaacatccg
     9241 cacgtcgcgg tcatcgtaaa acggagcgaa caggcccagc gaattggagc ccccgccgac
     9301 gcacgcgagc acggcgtcgg gcagacggtc ggttttttcc aggcattgcg cgcgcgcctc
     9361 gcggccgatc accgactgga aatcacgcac catcatcggg aacgggtggg gcccgacgca
     9421 cgagccgatg atgtaatgcg tatcgtccac cgtctgcatc cagacgcgca tcgcttcatt
     9481 ggtcgcgtcc ttcagggtgc gcgagccgct caggacgggc accacctcgg cgccgagcag
     9541 ccgcatccgg aagacgttga gctcctggcg ccgcatgtcc tcctcaccca tgtagaccgc
     9601 gcactccaga ccgagcagcg cgcaggcggt ggccgccgcg acgccgtgct gaccggcccc
     9661 ggtttcggcg atgatgcgcc gcttgcccag ccggcgcgcc agcagcgcct ggcccagcgt
     9721 gttgttgatc ttatgcgcgc cggtgtgcgc caggtcctcg cgcttcagaa aaatgcgcgc
     9781 cttgccgccg agctcctcgg ccagccgccg cgcttcatac agcggcgtgg gccggcccac
     9841 gtaatcggcc agcagcccgt tcaattcggc atgaaacgcg gcatcctcgc ggatggcgag
     9901 gtattcccgc tccagatcga gcagcgctcc catcagcgtt tcgggaacat agcgcccgcc
     9961 gaactcgccg aaatgaccgg tcttgtcggg tagatcgttg agcgccggcg gcgccgggag
    10021 gctgggtgac gacatgaggg ttcctgtctg atgggtgatg cgagggcccc gcgccccgtc
    10081 agagttgacc gagccggccg gcgtgcgcgg ggtccgttcc gattatcccc gtttcccccc
    10141 catcaggcaa tcagggagcc gcgcggttca gtcgccctcg cccggcagcg tggtcggagc
    10201 gtcggcgtcg agaccgaccg cggattccaa tccgggcggg aaccgtccgg gcaacggggc
    10261 cggcagcggg cttggccgcg gcagcggttc cagctccagc gtcgcggtcg tcgcgggcag
    10321 ttcatcccgc agccgttcgg ccagcgcggc gaacgcggcg gcgtccccga ccgagccggt
    10381 gcccttgagc aattcaaaca ggtccgaatc ggtcgacagc acgaccatcg tatcgtcgcc
    10441 gagcgagctt tcgtaggtcg ccatggtctg caggaaccgg taaaagtcgg ccgcctgtgg
    10501 actggcattg taggcctcgg catagatctc ggcggcgcgc gcatcggccg cgccgcggat
    10561 ctgctggacc tgcttgtagg ccagcgagcg gatctcgcgc agttcctgtt cctttcgccc
    10621 gcgcagcttg gccgcctccc ccatgccctg cgagcggaac atctcggcga gctgcttgcg
    10681 ctcgctgatc atccgctggt agatcgacgt ctgcacctgt tcattgtagt tcaggcgttt
    10741 gatccggatg tcgagcaatt ccacgcccag ttccgtcagg tagggggcgg cccgctgatg
    10801 gatttctcgt tcgacctggg tgcggcccac gctgatctga cccaattcgc ccaggttgag
    10861 cgacgggtcg tccagcacct catcgcgcac cggcacgcgc cccttcgtcg tacgcacgat
    10921 ctcgatcagg tcgtggcgcg ccaccgcctc gcgcgtcgcg ctgtccagaa tgtcgtccag
    10981 ccgcgactgc gccagccgct cgtcggccag ccgcaggtaa aactgcttgg gatcggcgat
    11041 gcgccatcgg ccatagacgt cgatcccgat gtagactttc tccttggtcg ccacgtcgtt
    11101 gggatcgccg tcccactcca gcacccgctt ctccagccga tgaaccttct ggatgaaggg
    11161 taatttgaac ttgaggccgg gcgtcgtcac ggcctcgccg acgatttctc cgaactgggt
    11221 caggataacc tgctcgcgct cgttgacggt gtagaacgtc tgcccggcga cgaccaggac
    11281 aacgatcagg atgacgccga ccagaatcca gcgggcggag aaggtcattg cgcccctcct
    11341 tccacacgtg ctggaggcga gtccagttgc agcagcggga ggatctgtcc ggccgcgtcg
    11401 tcgaggatga tcttgcgccc cagccgcggc agcagccgtc ccatcgtttc gaggaagatg
    11461 cgctggcgcg tcacctcggg cgcctccaca tactcgcgaa acagcgcggt gaaccgggcg
    11521 gcatccccct cggcttcatt cacgcgctgc gtggcatagc cgcgcgcctc gctgatcagt
    11581 tggtcggctt cgccatgggc ccgcggcact tcgcggttgt actcggccca cgcctcgttg
    11641 atcatccgct cgcgttcctg ctgcgcctgg ttgacttcat tgaacgaatc ctggaccgga
    11701 cccggcgggt tcacgtcctg caacgccacc tgctcgatgc ccagacccat ctgataatcg
    11761 ttgagtaatt cctgcagccg atcctgggcc acgcgctgga cctcctcgcg gccgatcgtc
    11821 aacacctcat ccaccgtccg gtcgccgaca acctcgcgca tcaccgcctc gctcgcgtag
    11881 cgcagcgtgt cgaccggatt gcgcacgttg aacagaaacg ccttcggctc gttgatccga
    11941 tactgcacga tccattccac caccacggtg ttgcggtcgc ccgtcaccat cagcgattca
    12001 tcggcgtaat tgcccggacg gtaatcggtg cgcacgtcga catgggcggt ccggaaacca
    12061 aactcttctt tcatctggcg gttgaccggc accagttcca cccgctcgat gccgagcggg
    12121 agcttgaacc gcaggcccgg ctcggtcatg cgcacaaact tgccgaagcg cagcacgacg
    12181 cccaactcct ccgcctgcac cgtgtagatc gacgacatca cgacgatgat cagcgcgatc
    12241 gcgccgaaca gcccggcgat cagccgaccc acccctttgg gcagcacaat gcgattgatt
    12301 ctgacgacgc gcgccatggc ctgaccttca ccgaacaaat cgtgcgcgga agagccgggg
    12361 gggacgtgcg aagccccacc gggcccccat tcaaagtgat ccaccgccca cgttcaacct
    12421 ctttccgccg cctccagtcg gcgctgccgg gccccttctc ggaactctcg accattttgg
    12481 tttgccatcc tgagcaaccc tcagcatcgt gatttggagg ctgactcagc ctggcagccg
    12541 cggaataagt gggcaaca
//
LOCUS       DAOPCO010000093        12405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5053_length_12405_cov_5.019352, whole genome shotgun sequence.
ACCESSION   DAOPCO010000093 DAOPCO010000000
VERSION     DAOPCO010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12405
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5053_length_12405_cov_5.019352"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..2324)
                     /locus_tag="PLS90_08190"
     CDS             complement(90..2324)
                     /locus_tag="PLS90_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPK02424.1"
                     /translation="MPPASQPPPPQSAAAERIEGVVVRITFQNPENGYSVVRVEPRGD
                     VPPAARAGDGVITVVGVLPGVEVGQALLCEGEWIRDPRFGMQFKANWFKPMLPSGVKG
                     IEAYLASGAIRGIGPVMARRIVAHFGERTFELIDADPRRLGEVPGIGPKTLAKLRAAW
                     TECRHDRELITFLGEHNISPAIAARLRKEYGDNALAIVRFNPYRLITDVRGIGFHRAD
                     EIARKIGLPPDAPERIDAALAHALERQADEGHTWQPREMLVDQVIALIGAEPERVQER
                     LDEAILKQRLVVDRVEEQDAVFLRPLHEAEARVAHCLRKMLAARRRLPQIDGVAALAD
                     FEQRARFRLAPAQRKAVLEFVKGGLLVLTGGPGTGKTTTIRSIIELFTQARMQVKLAA
                     PTGRAARRLSETAKLPAETIHRLLGYQAPLGTFGRDAANPIEAQLIIIDEASMLDAAL
                     AAHLFAAIAPGSCLLLVGDEDQLPSVGPGNVLGDIIASARLPLVRLTEVFRQAQASLI
                     VTNAHRINQGHMPLLNPPQPAPGEPEPDFFFIEREEPAQIIEAIRTLAAERIPRKFGL
                     DPAREIQLLTPMRRGELGVDALNRELRELLNPQATAPAAATVGGLAAGDRVMQTANNY
                     DKQVFNGDIGRVAHVNADTQELLIDFDGRAISYLRDELDQLVPAYAITIHKSQGSEYP
                     AVIIPIHTQHWIMLQRNLIYTAITRARRLVCLVGTKRALRRAVANATRARRLTALRAM
                     LQRD"
     gene            complement(2343..4184)
                     /locus_tag="PLS90_08195"
     CDS             complement(2343..4184)
                     /locus_tag="PLS90_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPK02425.1"
                     /translation="MIRCPKVPRRTPVAVLLLVCLPWLAPPVARGQAPGDPAEPSAAQ
                     SLVSALWVSMRTTPLRDPVKLDPFLGEISEMPFDEIILQVVLDGEAFFNSRVLPRAAG
                     LSDTFDPLETILTELHSAQRPIRVIAWLDLFSAGAENSAALLAPTHVRNAHPDWLSAD
                     RAARHVDAQGRTWLEPGLPEVMTHLEMVVADLVARYPVDGIYLGALHDPQADWGFHPA
                     LVERWQQSHGPGVLPNAADSRWVSFRANLYTQALRRLRDVARRARPGLPLSAGGETIG
                     APPASPEEFFGTSVYRDLHQDWSAWLRLQLLDRLYLKSFFDEVSSAEQFNKWMSTALA
                     IGHPAGAQVTIGVSGHLNESVQALAQLRRAAEAGADGLAIDDHSRIVQDTGARSLFLS
                     AAERTVFSPTYLQTLAQVREAQRLREQKAAESQAKTEQVLSATVPATTATSPTLAADP
                     DQPLEPFPTEQATSGAPATQPELAASTAERPHLAADQLELPPPPPPDLEEADGRLIPA
                     EEVPYEDASAPVEALPSPAGEDDETMRALEDLVEMRIRTRPPDSPTTIPAELLEPPVP
                     PAVRERRETIDRLLQGGELGKKRWGDIRPDDQAQRYLREQFSNIFTD"
     gene            4500..6074
                     /locus_tag="PLS90_08200"
     CDS             4500..6074
                     /locus_tag="PLS90_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK02426.1"
                     /translation="MPMTLAQRIAIQWIVLGALVWGLWRVANDPSARAVPQRRVHEDG
                     REVVTLWMGTIMGRWDELAMREAIRRFNDTHREIFIDAKLMPATVYGSKVNLAVASGR
                     PPDVVYNADELVQSNRQRSDVSELVAPIPESMISAETRAAWGPAILRAIRVEGKIVRF
                     PDSAFLNGMLLRGNLDYFREAGVDIEQYLEHGWDYETFTREMTKVQEAARRTTGREVY
                     AFGLNLANAHRLLYTTLLPPIIGAEARARSFLEYDERSGRYRVDPAITVDALAAPLRF
                     MQDLIHKDGLWGRKYLGMDFGQIGVEMFNRLDLVSILADTPGYSIYSQLYAEEEIRRG
                     VRERPFHATNIPAPTPRAGMAPVFSAGANGWGVMRRIPPLSDAHTRAALEVARYVASP
                     EVQALVFRMSKVRIHLWPDDAAVAALVTDVEDPIAADAWLSYLWGLYKDWMKNAATYH
                     ADAGDWPDPATRKDLLIRMGPDAPASYLTAGMGKQILEAVLFNQKAPEEAARELLAGM
                     QREIDEYYARNAPGAR"
     gene            complement(6109..7227)
                     /locus_tag="PLS90_08205"
     CDS             complement(6109..7227)
                     /locus_tag="PLS90_08205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02427.1"
                     /translation="MNHPEPTAAEPNPDARYRFGVVGAAMRAEYYLRIARALPDRFEV
                     TGAVARNPDRARAFSRRWDCPTFPTIDEMLKHTRPEFVITAVNWEQNAAVTAQLVELG
                     MPVLAETPPASTLEGLLELHALVQRRGGRVQVAEGVPLRPRHQAQLAVVAKGWLGRVT
                     QAQISVAHGYHGIALMRKLLGIGFEHCRVTARSFSSPLVKSLGPEGTPEQETTHDSQQ
                     YLFWFDFGDRLGVMDFTFDQYWSWVRNERTLIRGERGEIIGDTIYYLKDHKTPMRLEL
                     CPHRPDRTTIVEGYFLKGIQVGEDWVYTNPLVPANLPDDEIAGGVVMANMGKYVRTGV
                     PFYSLADGMQDHYLYLVAQRALGAEASLATETMPWATP"
     gene            complement(7737..10151)
                     /locus_tag="PLS90_08210"
     CDS             complement(7737..10151)
                     /locus_tag="PLS90_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02428.1"
                     /translation="MLRLDPKSKTLNLRHPAVKLENWQLGITANGNRLKSTQADVELL
                     DADLPHFRLKFPDLGLVWMVRGETDEEHGTLLLHSQLTNESNEPIALGKVYPLECPGR
                     VQIGQTDQNVVNLPLPVGQFGRKVGRITDLTTARASKIKFQFYNQDARLAFQVGFITF
                     QRANTEIHYNFDEQNGMRGLRAFCDFDGWKLQPGQSTSAETFILAVGDNPFRQLEHWA
                     DLAAAAAPCPPRVWEDAPIGWVGFSWVDPFTVERYEDVVLRNAEAIEHRLKGFGADYI
                     WVSIGNMEAGIAGDWLSWNERAFPSGCAYLVHRLRQYGLKLGFWVGPFWVGSNLKEIL
                     EELGEDALQHKPDGSRNIVCKKYAWGRPGFWPREERPDMYGLDPTHPRAHKFIRETFE
                     TYRRWGIRYYMLDFLSGGTGNMGGYDEDSPEVDSGAPASHPDADYLLYAKHHDPTVVS
                     GPEAYCKFLKVVRDAAGDDTYFLASTGPTVHNVGLMDGVRTGNDFGGGMMLYPETDFL
                     HYPGTYVIGDAHFWTGPLPALMSMATSYYTHRRLYLNDVNMLTIDKPVPITAARMYAT
                     IHGISGGPTMIGDDLDRMDEDRLAMIKKTLPRSPEIAVPVDLFDRVAPDYPRIFQRKI
                     KKPWGEFDVVSVFNFSSEPLVETVELSRLGLDPDAEYVTWEFWNEEYVGLVRGALRAC
                     VAPGSVSIYRLARNNGYPMLLATDMHMMMGEMEIDASHWDAAAGTLAGRALRPCGERG
                     NLYLWAPNGLEADDPTGLWLAKDNRDHSLVVKLPMVFGPEGSRDWSVRFIPLRKELPP
                     ERCQLI"
     gene            complement(10354..>12405)
                     /locus_tag="PLS90_08215"
     CDS             complement(10354..>12405)
                     /locus_tag="PLS90_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02429.1"
                     /translation="WIYDLGATEPKLYSAWGHPIRTYQTWTGAAPLAHGARPALMMIN
                     RNLPGTRVVTQDGEQAEILWSDVAGGVEDQYQADITIDRGAPNPFIDLDGDGLVEILS
                     IYNNEHGDGVSHLIVFRPTDGERLCEAPGVRVLTAEDLDSDGKPEIFLQENDTLRIAH
                     WTGSGFQDLWHAEGVEPVFQPTPSAGVLWGNKCSNSVDANPLVWRETEGSENFLLRFP
                     DGVWSCRLDAAAGDLARTSLITAHAALAPPEEPADARFNGLAVFARHDDTLTTVYEVP
                     FRRQYLAQPPLVGQLDGETRVLARDHQDALVSLSVDGDDTRRLLKMPQLGFGYSIEHP
                     MIADVDGDGTGEAVAIDGERGEPALAILNGDGAVQRRIISDSGAESLVLVAAGRLGPG
                     QGMWIAVRCAKVFTQPYVEAYNGKTGELLWRRENYGVYLDKPSKFMIHVPTAVWDYDE
                     DGVDDLLASSESYYGILDGATGEDLVPPDPPFVTAIPGHWGAYSNPIVADYLHRGRPQ
                     VFLSQAFGLAPLIDLDGKPIWHYGLSRDTTMRSHPGVADLDGDGKLEIVTTQADGLLR
                     AFAADPLDEKCPTCPADVELTEANHSAAVRWEFSLTPPLSDVVSADLDGDGMDELLVG
                     AGDGKLHALKDRNGQCDSLWAVELGRVVGSPVIADLTGTGKPVILVPESGGYLHALGG
                     N"
BASE COUNT         1936 a         3834 c         4548 g         2087 t
ORIGIN      
        1 ccgggtgacc ggtggtatcg ctgcgctcaa ccaccggcta ccttccggca tccctgcggg
       61 aggcggggat taacgcgtcc atccggggat cagtcgcgct ggagcatggc ccggagggcg
      121 gtgagacggc gggcgcgggt ggcgttggcg accgcccggc gcagcgcgcg cttcgtgccg
      181 acgaggcaga ccaggcggcg cgcgcgggtg atcgcggtgt agatcaggtt gcgctggagc
      241 atgatccagt gctgcgtgtg gatcgggatg atcacggcgg ggtattccga gccctgcgac
      301 ttgtggatcg tgatcgcgta tgcgggcacg agctggtcga gctcatcgcg cagatagctg
      361 atcgcgcggc cgtcgaagtc gatcaggagt tcctgagtgt cggcgttgac gtgcgcgacg
      421 cggccgatgt cgccgttgaa cacctgcttg tcgtagttgt tggcggtctg catgacgcga
      481 tcaccggccg cgaggccgcc gacggtcgcc gcggccgggg cggtggcctg cgggttgagc
      541 agctcgcgca gttcgcggtt gagggcgtcg acgcccagtt caccgcgccg catcggggtg
      601 agcagttgga tctcgcgcgc cggatcgagg ccgaatttcc gcgggatccg ctcggccgcc
      661 agcgtgcgga tcgcctcgat gatctgcgcc ggttcctccc gttcgatgaa gaagaagtcg
      721 ggctcgggtt cgcccggggc gggttgcggc ggattgagca acggcatgtg accctggttg
      781 atccggtgcg cgttggtgac gatgaggctg gcctgggcct ggcggaagac ctcggtcagg
      841 cggacgagcg gcagccgggc cgacgcgatg atgtcgccca ggacgttgcc cggcccgacc
      901 gaaggcaact ggtcctcgtc gccgacgagg agcaggcaac tgcccggcgc gatcgccgca
      961 aacaggtggg cggccagcgc ggcgtcgagc atcgaggcct cgtcgatgat aatcagttgg
     1021 gcttcgatcg ggttggcggc gtcgcggccg aatgtgccga ggggggcctg gtagcccagc
     1081 aagcggtgaa tcgtttcggc cggcagcttg gcggtctcgc tcagccggcg cgcggcgcgt
     1141 ccggtcggcg ccgccagctt gacctgcatc cgggcctggg tgaacagttc gatgatcgag
     1201 cggatcgtgg tcgtcttgcc ggtgccgggg ccgccggtga ggaccagcag tccccccttg
     1261 acgaactcga ggacggcctt gcgctgggcg ggggcgaggc ggaaccgggc gcgctgctcg
     1321 aaatcggcga gcgcggcgac cccatcgatc tggggcagcc ggcggcgcgc cgcgagcatc
     1381 ttgcggaggc aatgcgccac gcgcgcctcg gcctcgtgca gcggccgcag gaagacggca
     1441 tcctgctcct cgacgcgatc gacgacgagg cgctgcttga ggatcgcctc gtccaggcgc
     1501 tcctggaccc gctcgggttc ggcgccgatg agcgcgatga cctgatcgac cagcatttcg
     1561 cgcggctgcc aggtatggcc ttcgtcggcc tggcgctcca gggcgtgcgc cagcgcggca
     1621 tcgatgcgtt ccggggcatc gggcggcaag ccgatcttgc gcgcgatttc gtcggcgcgg
     1681 tggaatccga tgccgcgcac gtcggtaatc aggcggtagg ggttgaagcg gacgatcgcg
     1741 agggcgttgt cgccgtattc cttgcgcagg cgggcggcga tcgcggggga gatattgtgc
     1801 tcgcccagga aggtgatcag ttcgcgatcg tggcggcact cggtccaggc ggcgcgcagt
     1861 ttggcgaggg ttttggggcc gatgccgggg acttcgccga ggcggcgcgg gtcggcgtcg
     1921 atcaattcga aggtgcgctc gccgaaatgc gcgacgatcc gccgggccat caccgggccg
     1981 atgccgcgaa tcgcccccga ggcgaggtag gcctcaatcc ccttgacccc gctgggcagc
     2041 atcggcttga accagttggc cttgaactgc atgccgaagc gggggtcgcg aatccactcc
     2101 ccctcgcaga gcagcgcctg gccgacctcg acgccgggca ggacgccgac gacggtgatg
     2161 acgccgtcgc cggcgcgggc ggccgggggg acatcgccgc gcggttcgac gcgcacgacg
     2221 ctgtagccgt tttcggggtt ttgaaaggtg atccggacga cgaccccctc gatccgttcg
     2281 gcggcggcgg actgcggggg gggcggctgc gaagcggggg gcatgcgcgg caggtggccc
     2341 cctcagtcgg tgaagatgtt gctgaactgc tcgcgcaggt agcgttgcgc ctggtcgtcg
     2401 ggacggatgt cgccccagcg tttcttgccg agctcgcccc cctggaggag gcgatcgatc
     2461 gtttcgcggc gctcgcggac ggccggcggg accgggggct cgagcagttc ggccgggatc
     2521 gtcgttggcg aatcgggcgg tcgcgtccga atccgcattt cgaccagatc ctcgagggcg
     2581 cgcatcgtct catcgtcctc gccggcgggc gaggggaggg cctcgaccgg cgcggaggca
     2641 tcttcgtagg ggacctcttc ggccgggatc agccgtccgt cggcttcttc gaggtcgggc
     2701 ggaggcgggg ggggaagctc cagttgatcg gcggcgagat gcgggcgttc agcggtagat
     2761 gcggccaact cgggctgtgt cgccggcgcg ccggacgttg cctgttccgt ggggaacggc
     2821 tccagcggct ggtccgggtc ggcggccagg gtggggctcg tcgcggtcgt ggcggggacg
     2881 gtggccgaga ggacctgttc ggtcttggcc tgggattcgg cggctttctg ttcgcgcagg
     2941 cgctgtgctt cacgcacctg ggcgagggtc tgcaggtagg tgggggagaa aacggtccgc
     3001 tcggcggcgc tcaggaagag gctgcgcgcc cccgtatcct ggacgatgcg gctgtggtcg
     3061 tcaatcgcga gaccgtcggc gcccgcttcg gcggcgcggc gcagctgggc cagcgcctgg
     3121 accgattcat tcaggtggcc gctgacgccg atggtcacct gcgcgccggc cggatggccg
     3181 atggccaatg cggtgctcat ccacttgttg aattgctcag ccgaagagac ctcgtcgaaa
     3241 aacgacttca gatagagccg gtcgagcaac tggagccgca gccaggccga ccagtcctgg
     3301 tgcaagtcgc gatagacgct ggtgccgaag aactcctcgg gcgaggcggg gggggcgccg
     3361 atcgtttcgc cgccagcgga gaggggcaga cccggccgcg cgcgccgggc gacatcgcgc
     3421 aaccgcctga gcgcctgggt gtagaggttg gcccggaacg agacccagcg cgagtcggcg
     3481 gcgttgggca gcacgccggg gccgtggctc tgctgccagc gctcgacgag cgcgggatga
     3541 aaaccccagt cggcctgcgg gtcgtgcagc gcgccaagat agatgccgtc gaccggatag
     3601 cgcgcgacga gatcggcgac gaccatttcc aggtgggtca tgacttccgg gaggcccggt
     3661 tcgagccagg tgcgcccctg ggcatcgaca tgccgcgcgg cgcggtcggc cgagagccag
     3721 tcgggatggg cgttgcgcac gtgcgtcggg gcgagcaggg cggcgctgtt ctcagcgccc
     3781 gccgagaaga ggtccagcca ggcgatgacg cggatcggcc gctgggcgct gtgcagctcg
     3841 gtcagaatcg tctcgagcgg gtcgaaggtg tcgctcaggc cggcggcgcg cggcaggacg
     3901 cgcgagttga aaaaggcctc gccgtcgagg acgacctgca ggatgatctc gtcgaagggc
     3961 atctcgctga tttcgccgag gaaggggtcc aatttgacgg ggtcgcgcag cggtgtggtg
     4021 cgcatggaga cccagagcgc gctgacgagg ctctgcgccg ccgacggttc ggccggatcg
     4081 ccgggcgcct ggccgcgggc gacggggggc gccagccacg gcaggcaaac gagaagcaga
     4141 acagcgacgg gcgtccgacg cggaaccttg ggacaacgga tcatagcgaa accagagcga
     4201 gggtgatgaa tgtaaggcgg aatcctcgtc tctgaacaag gctaagcgac gggcgaatgg
     4261 aatgcaatat ggtaaaggga gggcggcggc ggggcgcggc gccccagggg gacgaccggc
     4321 ggcgggaggt cgggggaatc cgcggacccc gcgatggagg ggattttcgg tggcgggcgg
     4381 ggagcggagc ggcgtaatat actcgggtat catgcccagc gggcggattt cccgctttgc
     4441 ccgaaccgtg ccccgcgcgg ggcccacggg cccccgcctg tgtctgaccg gaggccatcg
     4501 tgccgatgac gctcgcccag cggattgcca ttcaatggat cgttctgggc gccctggtct
     4561 gggggctgtg gcgggtggcc aacgacccgt cggcgcgggc ggtgcctcag cggcgggtcc
     4621 atgaagacgg gcgcgaggtc gtcaccctgt ggatggggac gatcatgggg cggtgggacg
     4681 agctggcgat gcgcgaggcg atcaggcgct tcaacgatac gcatcgcgag atattcatcg
     4741 acgccaagtt gatgccggcg acggtttacg gcagcaaagt caatctggcc gtagcgagcg
     4801 gacggccccc cgacgtcgtc tacaacgccg acgaactggt ccagtccaac cgccagcggt
     4861 cggacgtcag cgagctggtg gcgccgatcc ctgagtcgat gatcagcgct gaaacccgcg
     4921 cggcctgggg cccggccatc ctgcgcgcga ttcgcgtcga gggcaagatc gtccgctttc
     4981 ccgacagcgc gttcctcaac ggcatgctgc tgcgcggcaa cctggattat ttccgggagg
     5041 cgggcgtcga catcgagcag tacctggagc acggctggga ctatgagacg ttcacgcgcg
     5101 agatgacgaa ggtccaggag gccgcgcggc gaacaaccgg gcgcgaggtt tatgccttcg
     5161 ggctcaatct ggccaacgcg cacaggctgc tctacacgac gctgctgccg ccgatcatcg
     5221 gcgccgaggc gcgcgcgcgg tcctttctgg aatacgatga gaggagcgga cgctaccgcg
     5281 tcgacccggc gatcacggtg gatgcgctcg ccgcgccgct gcggttcatg caggacctga
     5341 ttcacaagga cgggctgtgg gggcgcaagt atctcggcat ggatttcggc cagatcggcg
     5401 tggagatgtt caatcgcctc gacctggtga gcattctggc cgatacgccg gggtattcga
     5461 tttattcgca gctctatgcc gaggaagaga tccggcgcgg cgtgcgggag cggccgttcc
     5521 atgccacgaa cattccggcg ccgacgccgc gggcggggat ggcgccggtc ttttcggcgg
     5581 gcgccaacgg gtggggggtg atgcgccgga ttccgccgct gagcgacgcg cacacccgcg
     5641 cagcgctcga ggtcgcgcgc tatgtggcct cgcccgaagt gcaggcgctc gtcttccgga
     5701 tgagcaaggt gcggattcac ctgtggcccg acgacgcagc ggtggcggcg ctcgtgaccg
     5761 acgtcgaaga cccgatcgcc gccgacgcgt ggctctccta cctgtggggg ctatacaagg
     5821 actggatgaa gaacgccgcc acctatcatg ccgatgccgg ggactggccc gaccccgcga
     5881 cgcgcaagga tctgctgatc cggatggggc ccgacgcgcc ggcgagctac ctgaccgccg
     5941 ggatgggcaa gcagatcctg gaggcggtgc tattcaatca gaaggcgccg gaggaggccg
     6001 cccgcgaact gctggccggg atgcagaggg agatcgacga gtattatgcg cgcaatgcgc
     6061 cgggcgcgcg gtagccgcgg gccgtgctgc gggggaatcg ggcttcgctc agggggttgc
     6121 ccagggcatc gtttcggtgg cgaggctggc ctcggcgccc agcgcgcgct gcgcgacgag
     6181 atagaggtaa tggtcctgca tcccgtcggc gagcgaatag aacggcacgc cggtgcggac
     6241 atatttcccc atgttggcca tcacgacgcc gcccgcgatc tcgtcatcgg gcaggttggc
     6301 gggcaccagc gggttcgtgt agacccagtc ctcgccgacc tggatgccct tcaggaaata
     6361 gccttcgacg atcgtcgtgc ggtcggggcg atgcgggcag agttcgagcc gcatcggcgt
     6421 cttgtgatcc ttcaggtaat agatcgtatc gccgatgatt tcgccgcgtt cgccgcggat
     6481 cagcgtccgt tcattgcgga cccagctcca gtactggtcg aacgtgaaat ccatcacgcc
     6541 gaggcggtcg ccgaaatcga accagaagag atactgctgc gagtcgtgcg tcgtttcctg
     6601 ttcgggcgtt ccctccggcc cgagggattt gaccagcggg gagctgaacg agcgcgccgt
     6661 gacgcggcaa tgctcgaaac cgatcccgag cagcttgcgc atcagggcga tgccgtggta
     6721 gccgtgcgcg acggagatct gggcctgcgt gacgcggccg agccagcctt tcgcgaccac
     6781 ggccaactgc gcctgatggc gcggccggag cgggacgccc tcggcgacct gcacgcgtcc
     6841 gccgcggcgc tggaccagcg catgcaattc gagcagccct tcgagggtcg acgccggcgg
     6901 ggtttccgcc agcaccggca tgccgagctc gaccagttgc gccgtcacgg cggcattctg
     6961 ttcccagttg acggccgtga tgacgaattc cgggcgagtg tgtttgagca tctcgtcgat
     7021 cgtggggaag gttgggcaat cccagcggcg ggagaaggcc cgggcccgat cgggattgcg
     7081 ggcgacggcg ccggtgacct cgaagcggtc ggggagggca cgcgcgatgc gcagataata
     7141 ttcggcgcgc atcgccgccc ccacgacccc aaagcggtat ctggcatcag gattgggttc
     7201 ggccgccgtg ggttcggggt ggttcatttt cctgcgcctt tgtggtgaat tcggtcggag
     7261 ggattcgaga atttgttatc gaaccgatag atcggttctg tgaattgggg gatagcgacc
     7321 gtgagttgca gggccgcggg gcgtccctgc aacccacggc ctggatagcg aaccgctgac
     7381 gcggttcctg atgtcgcgcg acgtttctcg gttcgcgtgg ggcggatccg gatgtggggc
     7441 ggcatctgcc gtccctgcgg gactcttcaa tcattccatc ttttctcccg ccgatgaagt
     7501 gattgtcatt ccccaagttc agacgagctc cggccacccc ttccaggggt ttgatgcgtc
     7561 ggggcggcga cgcagggttc cggcccgcgg ccgtcaccct gcgctttatt ccagtcgtcc
     7621 gctccgcgga ctgagtggcg ggcgaacgtc tgccgtccct gaggggactg ggccgctggt
     7681 gggtgcggaa aggcctgagc cgtttgaatc ggtcattcac ggtccccgtg ccggggctag
     7741 atcaactggc accgttcggg ggggagttcc ttacgcaagg ggatgaagcg cacgctccaa
     7801 tccctgctcc cttcgggtcc gaagaccatc ggcaacttga cgacgagcga atggtcgcgg
     7861 ttgtccttgg ccagccacag gccggtgggg tcgtcggcct ccagcccgtt cggcgcccag
     7921 aggtagaggt tgccgcgctc gccgcagggg cgcagcgcgc gccccgcaag ggttcccgcg
     7981 gcggcgtccc aatggctggc gtcgatttcc atctcgccca tcatcatgtg catgtcggtg
     8041 gccagcagca tgggatagcc gttgttgcgg gcgaggcgat agatgctgac gctgccgggc
     8101 gcgacgcacg cgcgcaacgc gccgcgaacc agcccgacat actcctcgtt ccagaattcc
     8161 caggtcacgt attcggcgtc gggatcgagc cccaggcggc tcagctcgac cgtctcgacg
     8221 agcggctcgg aggagaaatt gaagaccgaa acgacgtcga attcgcccca cggcttcttg
     8281 atcttgcgct ggaagatccg cggataatcg ggggcgaccc ggtcgaacag atcgaccggg
     8341 acggcgattt ccggcgaacg cggcagggtc ttcttgatca tcgcgaggcg atcctcgtcc
     8401 atccggtcca ggtcgtcgcc gatcatcgtc ggaccgccgc tgatgccgtg gatcgtggcg
     8461 tacatgcgcg ccgcggtgat gggcaccggc ttatcgatcg tcagcatgtt cacgtcgttc
     8521 agatagagcc gccggtgggt gtagtacgac gtcgccatgc tcatcagggc aggcagcggg
     8581 ccggtccaga agtgggcatc gccgatgacg taggttcccg ggtaatgcag gaagtcggtc
     8641 tcggggtaga gcatcatgcc gccgccaaaa tcgttgccgg tccggacgcc gtccatcagg
     8701 ccgacgttgt gcaccgtggg gccggtgctc gccaggaagt aggtatcgtc gccggcggcg
     8761 tcgcgcacga ccttgaggaa tttgcagtag gcttccggac ccgagacgac ggtcgggtcg
     8821 tggtgcttgg cgtaaagcag atagtccgcg tcgggatggc ttgcgggggc gcccgaatcg
     8881 acctcggggg aatcctcgtc gtagccgccc atgttgccgg tgccgccgct gaggaaatcg
     8941 agcatatagt agcgaatgcc ccagcgccgg taggtctcaa aggtctcgcg gatgaacttg
     9001 tgggccctgg ggtgggtggg atcgaggccg tacatgtcgg ggcgctcctc gcgcggccag
     9061 aagccgggcc ggccccatgc atactttttg cagacgatat tgcgcgagcc atccggcttg
     9121 tgctgcaggg cgtcctcgcc cagttcctcg aggatttcct tcagattgga gccgacccag
     9181 aacggtccga cccagaagcc gagtttgagc ccgtactggc gcagccgatg gaccagatag
     9241 gcgcagccgc tggggaaggc gcgctcgttc cagctgagcc agtcgccggc gatgcccgct
     9301 tccatgttgc cgatgctgac ccagatgtag tcggcgccga agcccttgag ccggtgctca
     9361 atggcctcgg cgttgcgcag gacgacatcc tcgtagcgtt cgacggtgaa cggatcgacc
     9421 caggagaagc cgacccagcc gatcggggcg tcctcccaga cgcgcggcgg acagggggcc
     9481 gccgcggccg ccaggtcggc ccagtgctcc agttggcgga aggggttgtc gcccacggcc
     9541 aggatgaagg tttcggccga ggtggactgg ccgggctgaa gcttccagcc gtcgaaatcg
     9601 cagaaagcgc gcaggccgcg catcccgttc tgctcatcga aattgtaatg gatttcggta
     9661 ttggcgcgtt ggaacgtgat gaacccgacc tgaaacgcga gccgcgcatc ctggttgtag
     9721 aactgaaact tgatcttgct cgcgcgcgcc gtcgtgaggt cggtgatgcg gccgaccttg
     9781 cggccgaact gccccaccgg cagcggcagg ttcaccacgt tctgatcggt ctggccgatc
     9841 tgcacgcggc ccgggcattc gagcggatag accttgccga gcgcgatggg ctcgttcgat
     9901 tcgttggtca gctgcgaatg cagcaggagg gttccgtgtt cctcatccgt ttcgccgcga
     9961 accatccaga ccagtccgag atccggaaat ttgagccgga agtgggggag gtcggcatcg
    10021 agcagttcga cgtcggcctg ggtcgatttg aggcggttgc cgttggcggt gatccccagt
    10081 tgccagttct ccagcttcac cgccggatgc cgcaggttca gcgtcttgga tttcgggtcc
    10141 agtcgtagca tggccggttg ccgtttgcgg aaggcactgt ccgcaaactc cctttctatg
    10201 gatgtaagga gtgtgaatgt cgcgaggcga gactccccgc gcgcgggagg ttgctccgat
    10261 cgcgcgcggg agcccgattt tcaactattg ggagcgtctc cgcccaggtt cggcccttgc
    10321 ggaatgccgg agccgaccgg ccccggcgcc gtttcagttg ccgcccaggg cgtggaggta
    10381 gccgccggat tccggcacga gaatcaccgg tttgcccgtt ccggtcaggt ccgcgatgac
    10441 ggggctgcca accacccggc ccagctcgac ggcccagagc gaatcgcact ggccgttgcg
    10501 gtccttgagc gcgtgcagct tgccgtcgcc cgcgccgacg agcagttcgt ccatgccgtc
    10561 gccatccagg tcggccgaga cgacgtcgct cagggggggc gtcaggctaa actcccaacg
    10621 gaccgccgcg gagtggttgg cctcggtcag ctcgacgtcg gccgggcagg tggggcattt
    10681 ctcgtcgagc ggatcggcgg cgaacgcccg gagcaagccg tcggcctgcg tcgtgacgat
    10741 ctccagcttg ccgtcgccgt cgaggtccgc gacgccgggg tgcgagcgca tcgtcgtgtc
    10801 gcgcgacagg ccgtaatgcc agatcggctt gccgtccaag tcgatcaggg gcgccagccc
    10861 gaaggcctgg ctgaggaaca cctgcggccg gccccgatgg agatagtccg cgacgatcgg
    10921 gttggagtac gccccccagt ggcccggaat cgccgtaacg aacggggggt cggggggaac
    10981 cagatcctca ccggtcgccc cgtcgagaat gccgtaatag ctctccgagg aggccagcaa
    11041 gtcatcgacg ccgtcctcgt catagtccca cacggccgtg ggaacgtgaa tcatgaattt
    11101 gctcggcttg tccaggtaga cgccgtagtt ctcgcggcgc cagagcagct cgccggtctt
    11161 gccgttgtag gcctcgacgt agggctgggt gaaaaccttg gcgcagcgga cggcgatcca
    11221 catcccctgg ccgggaccga gccgaccggc cgcaaccagc accaggctct cggcaccgga
    11281 gtccgagatg atgcggcgct ggacggcgcc gtcgccgttg aggatggcca gggcgggttc
    11341 gccccgctcg ccgtcgatcg cgacggcttc acccgttcca tcgccgtcga catccgcgat
    11401 catcgggtgc tcgatcgagt agccgaaacc aagctgcggc atcttgagga gccggcgcgt
    11461 gtcgtcgccg tcgaccgaca gcgacacgag cgcgtcctga tggtcgcgcg cgaggacgcg
    11521 cgtctcgcca tcgagctgac cgacgagcgg cggctgcgcc aggtactggc ggcggaaggg
    11581 aacctcatag accgtcgtca gcgtgtcgtc gtggcgggcg aagacggcca acccattaaa
    11641 gcgggcgtcc gcgggctcct cggggggagc gagcgcggcg tgtgcggtga tcaggctggt
    11701 cctggccagg tcgcccgccg cggcatcgag gcggcagctc cagacgccgt cggggaagcg
    11761 cagcagaaaa ttctcggagc cctcggtctc gcgccagacc agtggattgg catcgaccga
    11821 gttggagcac ttgttgcccc agaggacgcc cgccgagggc gtcggttgaa agaccggctc
    11881 gaccccttcg gcgtgccaca gatcctggaa accggagccg gtccagtggg cgatgcgcag
    11941 cgtgtcattt tcctggagga agatttccgg tttgccgtcg ctgtccagat cctcggcagt
    12001 caggacgcgc acgccgggcg cctcgcagag ccgctcgccg tcggtcggcc ggaagacgat
    12061 caggtgggag acgccgtcgc catgctcgtt gttgtagatc gagaggattt cgaccaggcc
    12121 gtcgccgtcg agatcgataa acggattcgg cgcgccgcga tcgatcgtga tatccgcctg
    12181 gtactgatcc tcgacgccgc cggcgacgtc gctccagagg atctcggctt gttcgccatc
    12241 ctgggtgacg acgcgggtcc ccggcagatt gcggttgatc atcatcagcg ccgggcgcgc
    12301 gccgtgggcc agcggcgcgg cgccggtcca cgtctggtag gtccggatcg ggtgacccca
    12361 ggcggaatag agcttgggtt cggtcgcgcc caggtcgtag atcca
//
LOCUS       DAOPCO010000094        12387 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5061_length_12387_cov_4.805060, whole genome shotgun sequence.
ACCESSION   DAOPCO010000094 DAOPCO010000000
VERSION     DAOPCO010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5061_length_12387_cov_4.805060"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLS90_08220"
     CDS             <1..772
                     /locus_tag="PLS90_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPK02430.1"
                     /translation="GDRLVATSSMGAPLRFFPELSTDAPPTDARRERLEATRNWLETL
                     GAERLFLHLPLDADQLGSIGQLLESHGVQPAPDRLGNAGGAAWFDGVQSRMRVALPYF
                     PEREFTLSLWVKPDPQQPEPPREGRIVSAWAEVLDDPVRVSFSRDGLSAGVENGRIWR
                     TDAFPLSADRWTHAAIVRTDYDLRLFIDGALVDSVRIHHPPDPIHTRAQDLGIGWNPN
                     HAPDGEFFRGAVDDLRIYGRALGEDEIRRLAAPPDAFP"
     gene            complement(792..2105)
                     /locus_tag="PLS90_08225"
     CDS             complement(792..2105)
                     /locus_tag="PLS90_08225"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK02431.1"
                     /translation="MKSLVHWSILICILLAGCVRDDAVRAASAAAAEGAHRPNPNVVP
                     LAQDYVVVAQVPDARYFFHDPNMALLSDGSILVAAPCWSDDERSTIMKRSDDGGATWR
                     ELGRLPYEDATPFMIGDRLYMFVQAETWRDVSFVTSFDRGETWSEPSTIFTGEYWTIS
                     NSTVIKDGRFYLALSTKYPDQERKYQRLVMAVADLSQDLLDPAAWRLSNVIDYAPVPD
                     AICRWMFPHEVQVEKFNRPWSGVSWLEHNTVEVNGRIRVLSRCVIDGFSTANLCSVAD
                     LDETGGELKLSFTQYYPLPGGQNKFFILYDDVSRFYFLLSNLVTDSQDLFERHAQLKA
                     SGFAAPPGNERRWLFLHYSIDCLNWFPAGNVAHWPDRVTQSFMYPSAAIDGDDIILIS
                     RTSKDGPNQHDADLVTFHRIRDFRSLTLPLHPGPEAYLPPAQASR"
     gene            complement(2258..3799)
                     /locus_tag="PLS90_08230"
     CDS             complement(2258..3799)
                     /locus_tag="PLS90_08230"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPK02432.1"
                     /translation="MSCIIRLFAAVTATLLISTAMAATINVGPSGDYTTITEALQNAA
                     NGDEIVIAPGTYDEHVDLSAMSDVTVRGQNRDTTIIAIGAANDVNEGILLDGSTGIVI
                     EDLTINMSADNGVDFRASIQAMNKSTFTARRVTMTGGGRSVSLFRGVDMTLQDCILSN
                     VRRLEGLSAGTGSGIEIGSDEDWDATDAMPTTVHVENCQFINCERELFLNPYAVDIDP
                     GTEEDLRPRSRFGDLTFIGCTFTGAHATLDIVNHMMTGQWAPGSEILFEGCHWSDWFG
                     RLFSFDSFPTTDAPDRVIFRDCSFLGNTAASTNGSVLLLGYACPFVLENTVINAYAFE
                     SIYVQNQCRGLEVTHCTLSNRNDTVIGSRAVAVNPLVTIRNSLLFCPGGFTRPPVNSY
                     GTVTNIAYNIDYSIVNSGNPADFESTVTVVRGGNYTHNVPDPNYIKFIAAANEDFANQ
                     EVDLHLMEDSPAIDTALGMGFDHDVEGLYRPQGPAPDMGAYEFDKGDTEPGPTPNAAR
                     HWVVF"
     gene            complement(3918..5414)
                     /locus_tag="PLS90_08235"
     CDS             complement(3918..5414)
                     /locus_tag="PLS90_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02433.1"
                     /translation="MCHPSRLFALALFGLAGLLAHAAVITVGPTGDYATIGEAITNAA
                     DGDEIQIAAGSYDEAVEISGRQDLTLKGDPGGGTIIAIGAGTAANQGVLINGSTGIVL
                     QDLTIDMTADNGVEYRAAIQAMEKSTFTANNVSMTGGGRNVSLWRGAEAELIDCELTN
                     VSRSGGNGGGIEIGSDEDYTTNTAMYTTVEATGCLFQHTEYPVYINPFATLGNTVART
                     RVGSLTFRDCEFDSNGGASNITMRFMTGQWEPGSEVLFEDCKFSNWAGYNFHMDGFPM
                     PTQAPEHVTFRRCEMIKLDASTSNAALYCNYACPIILENTAIATWQSEVIYLGARNEG
                     LTVTHCTLANVNPVVIAARAMNATVDAVVRNSIIAVRTTTPNRPPLTGYNEGTPLHFD
                     LDYTISNSLRDDDDGIQETVTTPPTLGANYVWNNTDENYANFINWAHPATDMQLGDPS
                     PALGAGADLGYTDDVLGKSRPNPAATNPDLGAYESYLVPAELSEFQFD"
     gene            6387..7067
                     /locus_tag="PLS90_08240"
     CDS             6387..7067
                     /locus_tag="PLS90_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02434.1"
                     /translation="MKQHGFTLIELLIVVAIIAILAAIAVPNFLEAQVRSKVSRVHAD
                     MRTLATGLEAYRVDNNTLPAWFDPGAAYLPISAGVVLSTPVAYLTNPRMVDPFASQQN
                     LQSWTLAGGIPFLQIFTNDDSSALRTIEIDTLMRQAATNPTVLPRCFPLKNYIIISSG
                     PDHVDNTVGGYAPYAPADPLGSGVYDPTNGTVSNGDIFRMHNGRIPRGYIEPLDSTRT
                     MRLTDPYL"
     gene            complement(7131..7793)
                     /locus_tag="PLS90_08245"
     CDS             complement(7131..7793)
                     /locus_tag="PLS90_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK02435.1"
                     /translation="MACEQRLRIDGRPGGPLMTNSYRLLDRPTGVWALVDPTDDALDL
                     WRVELARTGPPACVLITHGHFDHIGGVAAVRRRYPELPVWAHPDTAPLLADARLNQAA
                     AIGLPCEPAAATHYYREGDRVVIGGSELRVIDAPGHCVGSVMLHGDGQLIAGDVIFQG
                     GVGRWDLPGADYATLARTIRDKVMTLPDDTRIFPGHGPATTVGRERATNPLVREMLAR
                     VP"
     gene            complement(7891..8799)
                     /locus_tag="PLS90_08250"
     CDS             complement(7891..8799)
                     /locus_tag="PLS90_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HPK02436.1"
                     /translation="MAPLHCRARRRPTSLPFLLLIAAVHGCLPSEAPRAATADAPTTS
                     AQSVAPHLDRVLAAYVDERGLVDYAALRADRQGLDAYVAWLARVEPSTLASWPRAGQM
                     AFWINAYNGLTLRLIVDHYPIATAGAALVEPDAPANSVRQIAGAFSKISWPIAGRRTT
                     LDQIEHRILRARFGDPRIHMALVRGARSCPPLRREPYAAETLDRQLDEQARWFLHSPR
                     GLRFNSATGRGTLYLSSIFRWHMDDFAPIKNDNGEKGLFAFLRQYGPPALDALQPRGT
                     VIRFMRFDWRLNEQPSREERPIAKGL"
     gene            9194..9269
                     /locus_tag="PLS90_08255"
     tRNA            9194..9269
                     /locus_tag="PLS90_08255"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9227..9229,aa:Lys,seq:ctt)
     gene            9830..11224
                     /locus_tag="PLS90_08260"
     CDS             9830..11224
                     /locus_tag="PLS90_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPK02437.1"
                     /translation="MPKSSTTPRFRFTKNDLLAVAVPAGKDYIEVEDTEIRVLRCRIH
                     RPLADGRPGKMTMLINKSVRGRHIRKTLGEYRPGAQGNKIEDFRAKARAELTKIDEDA
                     AKWLEGEAERKADEITLEGAFGLALKASQRGAMARRDWAHAQTAFLAWMKKHHPYVTT
                     WARLRRVHVREYLADQTPSARSIERGKAELSPTRKRLLLQPVAQTARYMWLEYELPNV
                     AERLGLSAKLAKTPAPVYLQDVLAFLDYLKDKAPVASLEAGAALAGLAGLQLLEVLRL
                     TWNKVDFDRGLVEISGEVKNRYRNRVIPIPARCVEALRRAYSNRPAEIVREIHGGHVV
                     VSPQGRPFTGSSWMNYGKIMRTCMREWNPAMRWTPKDLRNCLLTLGAIRGFGGDVLEQ
                     YCGHAPRTVTARNYVPRLASASIGEAAQLEEQMEVFRRLVVENVEREITISNEKAKAK
                     AEAGANVRTIGAEG"
     gene            11229..11948
                     /locus_tag="PLS90_08265"
     CDS             11229..11948
                     /locus_tag="PLS90_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02438.1"
                     /translation="MTVNFDEAIRIARQAAGAGFNAEALRADLAGLYQQRTEAGGESR
                     RGIASLPARDVAALHRLEGHLEKVLEGWQSLSPEADTAVKVHAGVKALFRIRAATGRA
                     FDSDECTELVAATGTHIDELIQHAGNLLTAIRVAANAGAADYSRRAPESSGCCLDDEG
                     RIKKIVKRRGGGARKDGKREAVRALAEAWERHTGEPRGWLVAGDGKPTRFGRFIRAAA
                     PDLNDRLDSLQNYLRKHQPSH"
     gene            complement(12157..>12387)
                     /locus_tag="PLS90_08270"
     CDS             complement(12157..>12387)
                     /locus_tag="PLS90_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02439.1"
                     /translation="FFSAARGCVGAVSVRGLGLACDPGVNVGCTPAKAIAHAERAKRK
                     PTAMQSDVNAIRSQSEHLGNVAHRKQGIDLSD"
BASE COUNT         2168 a         3987 c         4012 g         2220 t
ORIGIN      
        1 cggcgaccgg ctggttgcga cctcttccat gggggccccg ttgcgcttct ttcccgaact
       61 gagcacggac gcgcccccca ccgacgcgcg ccgcgagcgg ctggaagcca cccggaactg
      121 gctggaaacg ctcggcgccg agcgcctgtt cctgcatctg ccgctcgatg ccgaccagct
      181 tggcagcatc ggccagctgc tggagtcgca cggtgtgcaa cccgcacccg atcggctggg
      241 aaacgccggc ggcgcggcct ggttcgacgg cgtgcaatcc cggatgcgcg tggcgctgcc
      301 gtatttcccc gagcgtgagt tcacgttgag cctgtgggtg aagcccgatc cgcaacagcc
      361 cgagccgccg cgcgagggac ggatcgtctc ggcctgggcc gaggtgctcg acgatccggt
      421 gcgcgtctcc ttctcgcgcg acgggctctc ggccggcgtt gagaacggcc gcatctggcg
      481 taccgatgct ttcccgctat ccgccgaccg ctggacgcat gcggccatcg tccgcaccga
      541 ctacgatctg cggctgttca tcgatggcgc gttggtcgac tccgtccgca ttcatcatcc
      601 gcccgatccg atccacactc gggcgcagga cctggggatc ggctggaatc ccaaccatgc
      661 gcccgacggc gaattcttcc gcggcgccgt ggacgatctg cggatctacg gccgggcgct
      721 cggcgaggat gaaatccgcc ggctggcggc gccgcccgac gccttccctt gaacggcgga
      781 tcaaccttga atcaacggct ggcctgagcg ggcgggagat acgcctcagg tccggggtgc
      841 agcggcagcg tcaggctgcg gaagtccctg atccggtgaa acgtgaccag atcggcgtca
      901 tgctggttgg ggccgtcctt cgacgtgcgt gaaatcagaa tgatatcatc gccgtcgatc
      961 gcggccgagg gatacatgaa cgattgcgtg acccggtcgg gccagtgcgc gacgttgccg
     1021 gccgggaacc agttcagaca atcgatgctg tagtgcagaa acagccagcg gcgttcattg
     1081 cccggcggcg cggcgaatcc gctcgccttc aactgggcat gccgctcaaa cagatcctgg
     1141 gaatcggtca ccagattgga caagaggaaa tagaagcgac tcacatcgtc gtagaggatg
     1201 aaaaacttgt tctggccgcc gggcagcgga tagtactgcg tgaagctgag tttcagctcg
     1261 ccgccggtct catcgagatc ggcgaccgaa cagagattcg cggttgaaaa gccgtcgatt
     1321 acgcagcgcg acagcacgcg gatgcgcccg ttcacctcga cggtgttgtg ctccagccag
     1381 gaaacgccgc tccacgggcg gttgaatttc tcgacctgga cctcatgcgg aaacatccag
     1441 cggcagatcg cgtcgggcac gggggcgtaa tcgatcacgt tggacagccg ccaggcggcc
     1501 ggatcgagca ggtcctggct cagatcggcc acggccatca ccagccgctg gtatttgcgc
     1561 tcctgatcgg gatacttggt tgaaagcgcg aggtagaagc ggccgtcctt gatcacggtc
     1621 gaattggaaa tcgtccagta ctcgccggtg aagatggttg acggttcgct ccacgtttca
     1681 ccccggtcaa agctggtgac aaacgacaca tcgcgccatg tttcggcctg aacgaacatg
     1741 tagagccggt cgccgatcat gaacggcgtg gcgtcctcgt agggcagccg gcccagctcg
     1801 cgccaggtcg cgccgccatc gtcgctgcgc ttcatgatcg tcgagcgctc gtcgtccgac
     1861 cagcaagggg cggcaaccag aatcgaccca tcggacagca gggccatgtt gggatcatgg
     1921 aaaaagtaac gtgcatccgg gacctgcgcg acgacgacat agtcctgcgc cagcgggacg
     1981 acgttcgggt tcggacggtg ggcgccctcg gcggcggcgg ccgacgcggc gcgaacggcg
     2041 tcatcgcgca cgcatcccgc cagcaggatg caaatcagga tcgaccaatg aaccagggat
     2101 ttcattcgct ggagcctttg cagagtcatg ttggaacgga gtggcctttc gccggccgga
     2161 gaggtcggcc ggcgaaaggc cgtggagggg agggcggggg cacggcgccg ggggattccg
     2221 tcggatcaag ccacccgcat cgggatcatg gtcagactta gaacaccacc cagtgacgcg
     2281 ccgcattggg cgtgggaccg ggctccgtgt cccccttgtc gaattcatag gcgcccatgt
     2341 cgggggcggg gccttgcggg cgatagagcc cctcgacatc gtggtcgaat cccatcccaa
     2401 gcgccgtatc gatcgcgggc gaatcctcca tcaggtgcag atccacttcc tgattggcga
     2461 agtcctcgtt ggccgccgcg atgaacttga tgtaattggg atccgggacg ttgtgcgtgt
     2521 agttgccgcc gcgcaccacg gtgaccgtcg actcaaagtc cgcgggattg cccgaattca
     2581 cgatcgaata gtcgatgtta taggcgatat tcgtcaccgt cccgtaggaa ttgaccggcg
     2641 gccgcgtgaa cccgccgggg cagaacagca gcgagttgcg gatcgtgacc agcggattga
     2701 cggcgaccgc ccggctgccg atcaccgtat cgttgcggtt cgacagcgtg cagtgcgtca
     2761 cctccagacc ccggcactgg ttctgcacat agatgctctc gaaggcatag gcattgatca
     2821 ccgtgttctc cagcacgaac gggcaggcat agcccagcag caggaccgag ccattggtgc
     2881 tcgccgccgt gttgcccagg aacgagcagt cgcggaagat cacccggtcc ggcgcgtcgg
     2941 tcgtcgggaa gctgtcgaac gagaacaggc gcccgaacca gtcgctccaa tggcagccct
     3001 caaagaggat ctcgctgccg ggggcccact gaccggtcat catgtggttg acgatgtcga
     3061 gcgtggcgtg cgcgccggtg aacgtgcagc cgatgaacgt caggtcgccg aagcgcgacc
     3121 gcggccgcag gtcttcctcc gtaccggggt cgatgtcgac cgcatagggg ttgaggaaga
     3181 gctcgcgctc gcaattgatg aactggcagt tctcgacgtg aaccgtcgtc ggcatcgcgt
     3241 cggtcgcgtc ccagtcctcg tcgctgccga tctcgatccc gctgccggtg ccggccgaca
     3301 gtccctcgag ccgccgcaca ttgctcagga tgcaatcctg caacgtcatg tcgacgccgc
     3361 ggaagagcga gacgctgcgg ccgccgccgg tcatcgtcac gcgccgggcg gtgaacgtcg
     3421 atttgttcat tgcctggatg ctcgcgcgga aatcgacgcc gttgtcggcg ctcatgttga
     3481 tcgtcagatc ctcgatcacg atgccggtcg agccgtcgag caggatccct tcattgacgt
     3541 cgttggcggc gccgatggcg atgatcgtcg tgtcgcggtt ctggccgcgc accgtcacat
     3601 cgctcatggc gctcaggtcg acgtgctcgt cgtacgttcc cggcgcaatc acgatctcat
     3661 cgccgttggc cgcgttctgc agcgcctcgg taatggtcgt gtaatcgccc gaggggccga
     3721 cgttgatcgt ggccgccatc gcggtcgaaa tcagcagcgt cgccgtcacg gctgcaaata
     3781 gtcgaatgat gcacgacatg cctggtcttc ctttccccgg ttattcccgg ttgacacaat
     3841 gggggttaca gggtggcgtg gccggcggcg tgctccgccg cgcgccgccg gccgcttcga
     3901 acgatttccg tccgcgctca gtcgaactgg aattccgaca gctcggccgg caccagatag
     3961 gactcgtaag cgcccagatc gggattggtc gccgccggat tgggacggct cttgccgaga
     4021 acgtcgtcgg tatagcccag gtcggcgccc gccccgagcg ccggcgacgg atcgcccagc
     4081 tgcatgtcgg tcgccggatg cgcccagttg atgaagttgg cgtagttctc gtcggtgttg
     4141 ttccagacgt aattggcgcc gagcgtgggc ggcgtcgtca ccgtctcctg gatgccgtcg
     4201 tcgtcgtccc gcagcgaatt cgagatcgtg tagtccaggt cgaaatgcag cggggtcccc
     4261 tcgttgtagc cggtcagcgg cggccggttg ggcgtcgtcg tccgcacggc gatgatcgag
     4321 ttgcgcacga cggcgtcgac cgtcgcgttc atggcccgcg cggcgatcac gacggggttg
     4381 acgttggcca gcgtgcaatg cgttaccgtc aagccctcat tgcgcgcccc cagatagatc
     4441 acttcggatt gccatgtcgc gatcgccgtg ttttccagga tgatcggaca ggcatagttg
     4501 cagtagagcg cggcgttgga ggttgaggca tccagcttga tcatctcgca gcggcggaac
     4561 gtcacgtgtt ccggcgcctg cgtcggcatc gggaaaccgt ccatgtggaa gttgtagccg
     4621 gcccagttgc tgaacttgca gtcctcgaac agcacctcgc tgcccggttc ccactggccc
     4681 gtcatgaagc gcatggtgat gttgctggcg ccgccgttgc tgtcgaattc gcaatcgcgg
     4741 aacgtgaggc tgcccacgcg cgtgcgcgcc accgtgttgc ccagcgtcgc gaacggattg
     4801 atgtagaccg ggtactcggt gtgctggaac aggcagccgg tcgcttcgac cgtggtgtac
     4861 atcgccgtgt tggtggtgta atcctcgtcg ctgccgattt caatgccgcc cccgttgccg
     4921 ccggagcgcg aaacgttagt cagctcgcag tcgatcagct cggcctcggc gccgcgccag
     4981 aggctgacgt tgcggccgcc gccggtcatg ctgacgttgt tcgccgtgaa cgtcgacttc
     5041 tccatcgcct gaatcgcggc gcggtattcg acgccgttat cggccgtcat gtcgatcgtc
     5101 aggtcctgca gcacgatccc ggtcgagccg ttgatcagga cgccctgatt ggccgccgtg
     5161 ccggcgccga tggcgatgat cgttccgccg ccggggtcgc ccttcaacgt cagatcctgg
     5221 cgcccgctga tttcgacggc ttcatcgtag ctcccggcgg cgatctggat ttcgtcgccg
     5281 tcggcggcgt tggtaatcgc ctcgccgatc gtcgcgtaat cgcccgtggg gccgaccgtg
     5341 atcaccgccg cgtgggccag caggccggcc agcccgaaca gggccaaagc aaagagtctg
     5401 gatggatggc acatgtggag gtcctccctg tcgtttcaaa ggcagatgcg tgcggagagc
     5461 cgccgcggcg aggcgtggcg cgcgtcgtcg ccgccgggcc ggacgctctg ctggagtggc
     5521 gcgtgcatcg aatcgtcccc ggattgatgc atcgaccggt tccgtcggcc cgtccggcgg
     5581 cggcatcgtc ccgactgcgc gatgccgaac gccctcccgt gcggaccgat cctcccgtca
     5641 agttgcccca caggccgcga aacaacataa aaaccttgca cgcccgcgca catcccctca
     5701 tcggcagagc cggcagcctc atcgcgaggc aacgtccttt caggtcgtgt ccacctcctt
     5761 tcactggggc gggcggcctc ggggctgatt cggtgattaa cgttaatgaa tataggcctg
     5821 ccttgcccga cctgtcaagc ccttttctgt cccgcggcgc ctgatttccg ccgggctccc
     5881 cggcgcccat ccgtcagccc gcccgacccc ctccaggctc cgctggccgg tgtgcccgcg
     5941 acggagctcg agcgcactct gcgccgcgct gaagggcttg cattacccca tttcccgcgc
     6001 cctcaccggc tgcataccgg caaggcgagc gggttaggaa caggacctgg cggatccggc
     6061 cagacgggga ctgcgccatc cccggcggat cacggcgggg gcccccgggt ggccgcgggc
     6121 cgtgcgccaa cgaccggagc cagccctccc gcgcccgcgg cggaggaaaa aatgagaaac
     6181 cccttgacaa ggtttgtcgg gcgggccgat actgagctgg attcatagtt aacgttaatg
     6241 gatcgcggct ggggagggcc gctcgtgacc aatctcttca gttgtcgacc ccgcgtccgg
     6301 cggccccgcc cacgcccgcg ccggcgagtc cgccagccgc gattgaaacc atcattccct
     6361 tgaagcgccg gaggagacgc ctgtccatga agcagcacgg attcactttg atcgagcttc
     6421 tgatcgtcgt cgcgatcatc gccatcctgg ccgccatcgc ggtgcccaat ttcctcgagg
     6481 cgcaggtccg ctccaaagtc tcgcgcgtcc acgccgacat gcgcacgctg gccaccggcc
     6541 tggaagccta ccgcgtcgac aataatacgc tgccggcctg gttcgatccc ggcgccgcct
     6601 atctccccat ctcggccggc gtggtcctct ccacccccgt cgcctatctg accaacccgc
     6661 gcatggtcga cccgttcgcc tcgcagcaga acttgcagag ttggacgctc gccggcggca
     6721 tcccgtttct ccagatcttc accaacgacg attccagcgc cctgcgcacc atcgagatcg
     6781 acacgctgat gcgccaggcg gcgaccaacc cgacggttct cccccgctgc ttcccgctga
     6841 agaactacat catcatcagc tcgggccccg accacgtcga caacaccgtc ggcggctacg
     6901 ctccctacgc cccggccgat ccgctcggca gcggcgtcta tgacccgacc aacggcaccg
     6961 tgagcaatgg cgacatcttc cgcatgcaca acgggcggat tccgcgcggc tacatcgaac
     7021 cgctcgactc cacccgcacc atgcggctaa ccgatcccta tctgtaggat gattggcgga
     7081 tcaggggcag cgagagccag gacttccgcg cggcggccgc ccgagcttcc ttacgggacc
     7141 cgcgccagca tctcccgcac cagggggttg gttgcgcgtt cccgaccgac ggtcgtggcc
     7201 gggccgtggc ccgggaagat ccgcgtgtcg tcgggcagcg tcatcacctt gtcccggatc
     7261 gtgcgcgcca gcgtcgcgta atcggcgccg ggcaggtccc agcggccaac gcccccctga
     7321 aagatcacgt cgccggcgat gagctgaccg tctccgtgga gcatcaccga gccgacgcag
     7381 tgccccggcg cgtcgatcac ccgcagctcg ctcccgccga tcaccacccg gtcgccttcg
     7441 cggtaatagt gcgtggccgc ggccggttcg cagggcaggc cgatcgccgc cgcctggttc
     7501 agccgggcat cggccagcag cggcgccgtg tcgggatgcg cccagaccgg cagctcgggg
     7561 tagcgccggc ggacggccgc cacgccgccg atgtgatcga agtggccgtg ggtgatcagc
     7621 acgcacgccg gcggtcccgt gcgcgccagt tccacgcgcc agaggtcgag cgcatcgtcg
     7681 gtcggatcca ccagggccca cacgccggtc ggccgatcca gcagccggta gctgttggtc
     7741 atcagcggcc cgcccggccg cccgtcaatc cgcagtcgtt gttcacaagc catctcgttc
     7801 cactatctcc aaaagtcgtt cctcgtcctc gcccctcgtc ctcggctttc gaaaaccggc
     7861 cattttcccc cttcgttgtg acccgcgggt tcataagcct ttcgcgatcg ggcgttcctc
     7921 ccgcgacggt tgttcgttca atcgccaatc gaaacgcata aaccggatga ccgtgccgcg
     7981 cggttgcagc gcatcgagcg cgggcgggcc gtactgcctc aggaaggcga ataagccctt
     8041 ctcgccgttg tcgttcttga tcggcgcgaa gtcgtccatg tgccagcgga agatcgacga
     8101 gagatacagc gttccgcggc ccgtcgccga gttgaagcgc agcccgcgcg ggctgtgcag
     8161 gaaccatcgc gcctgctcgt ccagttgccg gtcgagcgtt tcggccgcat agggctcgcg
     8221 ccgcagcggc gggcaactgc gcgccccgcg gacgagtgcc atgtgaatcc gcggatcgcc
     8281 gaaacgcgcg cgcaggatcc ggtgttcgat ctgatccagc gtggtgcgcc ggccggcgat
     8341 cggccaactg atcttgctga acgccccggc gatctggcgc acgctgttgg cgggcgcatc
     8401 gggctcgacg agcgcggcgc cagccgtcgc gatcggataa tgatcgacga tcagccgcag
     8461 cgtcagcccg ttgtaggcgt tgatccagaa ggccatctgc ccggcgcggg gccacgaggc
     8521 aagggttgac ggttcgaccc tggccagcca ggcgacgtag gcgtccagcc cctggcgatc
     8581 ggcccgcagc gccgcgtagt cgaccagccc gcgctcatcg acataggcgg cgagcacccg
     8641 atccaggtgc ggcgcgacgc tctgcgcgct cgtcgtcggc gcatccgcgg tcgcggcccg
     8701 cggcgcttcc gatggtagac atccatgcac ggcggcaatc agcagcagga acgggagcga
     8761 agtcgggcgg cgtctcgccc ggcaatgcaa cggggccatg ggttcctctt ggagcgggat
     8821 gcaaccgaac aaagtccggg gaagcgggca gcgggggtcg cgagccgccg gcgcgacgcc
     8881 gcgcgggccg ccgcgtcgcc gtgcccgtgc gccggccgcg agccgtcgcg tggcctcctt
     8941 cattctagat caatctcccg ttcccggcac ggattcgttc gcgggaaagt tgggcttccg
     9001 gttcgtcgcc caggcgaaaa ggcgtccctc gttctcccac cgcggccccg aggcgcgcct
     9061 caaacgcgat aaatggtctt gatcttgacg cggattctgg aaaaataggc tcatctgcgt
     9121 tcggcggacg gaggcggatg gatcctcccc gatccccacc ccccgccgcc cggccgacgt
     9181 tgcgtgtgaa cctgggtcat tagctcaatt ggtagagcag cggactctta atccgttggt
     9241 tataggttcg agtcctatat gacccaccat tgaaaataaa gggcgttcga gtgatgacga
     9301 gccagacacg cgcccgaaat ggctggaaac ggccaggaat cgccaaggcc ggctggcacg
     9361 aaataacctc gatcttataa ccaccatgca cccaagaagc cccccacgtg ggggcttttt
     9421 gctatccggt gcgagctcga gcgatcaagt ggcaagacct gcgggagaac ttgattccgc
     9481 tttgcgatag gttccggtgt ggagaacctc tgggctcacg tagagccgta ctcaaaccat
     9541 tccggcgaat tcgtactatg ccatctgatc cggtgatttc gtactatgcc atataagttt
     9601 tttcagtatt ttaaagtaat tattttcagt ggcgtccccg gcaggattcg aacctgcgac
     9661 ctacggatta ggaatcccaa aaagtgccac tttcggcgtc tttcaatgga aaacggcccc
     9721 ttactttttc ccttgcaatc cgggcgattt actcttacac tccctttcgt tgtcctatag
     9781 gtcattgcgt tgagcaatgg aaactgaatg gaaaatgagc gacctacgga tgccgaaaag
     9841 cagcaccaca ccacgattca gattcacgaa aaatgatctg ctggccgtgg ccgttccagc
     9901 cggcaaggat tatatcgagg tcgaggatac ggagatccgc gtcctgcgct gccgcatcca
     9961 tcgcccgctg gctgatggcc ggccgggcaa gatgacgatg ctgatcaaca agtcagtgag
    10021 gggccgccac atccgaaaga cgcttgggga gtatcggccg ggggcgcagg ggaacaagat
    10081 tgaagacttc cgggctaagg cgcgggctga attaacaaag atcgatgagg acgctgccaa
    10141 gtggcttgag ggtgaggccg agcgcaaggc cgacgagatc acgctcgaag gcgccttcgg
    10201 gctggcgctc aaggcatcgc agcggggggc catggcgcgg cgcgattggg cccatgcgca
    10261 gacggcgttc ctggcatgga tgaaaaagca tcatccctac gttacgacat gggcgcgcct
    10321 gcgcagggtg catgtgcgcg aatacctggc cgatcagacg ccgagcgcgc gcagcatcga
    10381 acgcgggaag gccgaactct cccctacccg caagcggctg ctcctgcaac ccgtcgctca
    10441 gacggcgcgg tatatgtggc tggagtatga actcccgaac gtggccgaac ggctggggct
    10501 ctctgcgaag ctggccaaga ctccggcgcc ggtttacttg caggatgtgc tggcgttcct
    10561 tgactacctg aaggacaagg cgccggtggc gtcccttgag gccggtgcgg cgctggctgg
    10621 gctggctggg ctgcaactgc tggaggtcct gcgcctgacg tggaacaagg tcgacttcga
    10681 tcgggggctg gtcgagatca gcggcgaagt gaagaatcgc taccgcaacc gcgtcattcc
    10741 gatccccgcg cgctgcgtcg aggcgctcag gcgggcttat agcaaccggc cggcagaaat
    10801 cgttcgggag atccatggcg gacatgttgt cgtgtcgcct caagggcgac cgttcaccgg
    10861 aagctcatgg atgaactacg gcaagatcat gcgcacctgc atgcgggaat ggaatccggc
    10921 gatgcgctgg acgccaaagg atctgcgcaa ctgcttgctg accctggggg ccatccgcgg
    10981 cttcggaggc gatgttctgg agcaatactg cgggcacgct ccgcggacgg tgacggcgcg
    11041 gaactacgtc ccgcggctgg cttctgcgag tattggcgaa gcggcacagc ttgaggagca
    11101 gatggaagtc ttccggcggc tggtggtcga gaacgtcgag cgggagatca cgatcagcaa
    11161 cgagaaagcc aaggcgaagg ccgaggccgg cgcgaacgtc cggactatcg gcgcggaggg
    11221 gtgaggcaat gactgtaaac ttcgatgagg caatcaggat cgcgcgacag gcggcgggcg
    11281 cgggcttcaa tgccgaggcg ctacgggccg atctggccgg gctctatcag caacgaaccg
    11341 aggcgggcgg cgaatctcgg cggggcatcg caagtctacc ggcgcgcgac gtggccgcgc
    11401 tccatcgact cgagggccac ctcgagaagg ttctggaggg ctggcagtcg ctttcgcccg
    11461 aggccgacac ggccgtcaag gttcacgcgg gggtgaaggc gctattcagg attcgggcag
    11521 cgacgggaag ggctttcgat tctgatgaat gcacagaact ggtggccgcg accggaacgc
    11581 atatcgatga gttgattcag cacgcgggaa atctgttgac cgccatccga gtggccgcca
    11641 atgccggggc cgcggactat tcgcggcgcg cgccggaatc aagtggttgc tgcctggacg
    11701 atgaggggcg aatcaagaaa atcgtcaagc gcaggggcgg cggagcgaga aaggacggga
    11761 aacgcgaggc agtccgggcc ttggctgagg catgggagcg acacacgggc gaaccgcggg
    11821 gatggctggt tgcgggggat ggaaaaccga cccgcttcgg tcgattcatc cgcgcggcgg
    11881 cgcccgatct gaacgacagg ttggactcgc tccagaacta cttgcgaaaa caccaaccaa
    11941 gccactgaat caaacgacat tccatgattc gagggcctgg ccgatgccgg gcctttttct
    12001 ttcccctctg catcggtatg taatggccgt tcgattttca tgcggccgtt acatacctat
    12061 cgtgtttaat atgtcttgca caggttgacg ccctcctctc ccctctggta accttgtcac
    12121 agactttcga ggggagaaaa cctgatgact tcaccttcaa tcactcagat cgatgccctg
    12181 ctttcggtgc gcgacgttgc ccaagtgttc ggactgactc cgaatagcat tcacgtcgct
    12241 ctgcatcgcg gtcggtttcc gcttcgcccg ttccgcgtgg gcaatcgcct tcgctggcgt
    12301 gcatccgacg ttcacgcctg gatcgcacgc gaggccgagg ccgcgaacgc tgacggcgcc
    12361 gacgcacccg cgcgcggctg aaaaaaa
//
LOCUS       DAOPCO010000095        12381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5063_length_12381_cov_4.211261, whole genome shotgun sequence.
ACCESSION   DAOPCO010000095 DAOPCO010000000
VERSION     DAOPCO010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5063_length_12381_cov_4.211261"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLS90_08275"
     CDS             <1..696
                     /locus_tag="PLS90_08275"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK02440.1"
                     /translation="LARLPGHGTTPIDFADQTPETLYAGALHELRALRAEYDEVTVIG
                     FSMGGTLATLLAAREPVDRLVLIAPYYGVTYAWYYVLPPHVWHQMLGWSMPWLYRWEC
                     FTKLNRPEGRTGLYSYKYVPSRGVETLFALGEEARAAGLLEAIQCPVLLVMSEGDQAS
                     CPVAARAAFARIGSPQKQARWFPGRSNHHLLRDWDGEAAKGAILEFLGERRVDASSQP
                     RGAETDSMASSRS"
     gene            complement(648..2003)
                     /locus_tag="PLS90_08280"
     CDS             complement(648..2003)
                     /locus_tag="PLS90_08280"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPK02441.1"
                     /translation="MARKDPAYKWEIANAFKGQRVKTYLLRLTSQTWRSGQEVNAPVW
                     THWLLVNVPDSLYQDTALLLIAGGANNDPTPEAADPLTRDLALATGSVTAMLYQVPNQ
                     PLSFTGEGGSDRVEDAIIAWSWDKYLATGDETWPAQLPMVNSVVRAMDAIQEFMLSTE
                     GGEFPLQDFFLTGASKRGWTAWLTAAVDPRVMGIAPLVIDVLNMRENIRLHHAAYGFY
                     APTLRDYEAAGIMDRMETPATNALIQIVDPFAYRDRLDMPKLLIYAAGDQFFLPDSSR
                     LYFSRLRAPKYLRYIPNADHSLQNTNVRESLLGFYRAVLNDMPLPTFDWQLEPGGALR
                     VFTRVDPLEVNLWQADNPAARDFRLETIGPAWTKTSLPYQDDGIYRADPGAPPTGWRA
                     YFLEVVFDSGFPEPYIFTTDVNVAPATLPFAADDDQPTTETAPLQDLDDAIESVSAPR
                     G"
     gene            complement(2174..2590)
                     /locus_tag="PLS90_08285"
     CDS             complement(2174..2590)
                     /locus_tag="PLS90_08285"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HPK02442.1"
                     /translation="MTPPDPSPPPRECDVEILPPADGPLPAQRSSAPRPAPHPLRYLF
                     SYVPGVTTLVALWLYLLDEHASSLHKLSIVLTFLYFVWPLDLLKDLFVGLGYLDDLAI
                     FYGLVRFIGSETLRPYRLEARRWLRGLPPLNPHIAP"
     gene            complement(2592..3530)
                     /locus_tag="PLS90_08290"
     CDS             complement(2592..3530)
                     /locus_tag="PLS90_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02443.1"
                     /translation="MPVLTRQQFYPFDETDFLRSTVDLGRIKRSYFVGEPVAVPLVLS
                     NHTRFPVTIETNFVPVSQLQVYIQPENDREERYPGAHLPELYGTVEIPLRPLASSTTQ
                     VHLWADRESSVGLAFEAPGEYRIRIVQTASVKEGGAALRNIQLNYPEFTLRIRETPDE
                     LKPLIERLKQIKGFISMQLGRLPPEMSIEEAEEIVRTAPASPLTAYLYNCLGARYQLE
                     FIDDPSRRDLADKALFYYQSASLSDSPIQIEILLNLLRFFDRLDVPQGAADTARRLVE
                     TMPSNQRKNLGSHSLVQKYLALYDDIEPMTFWTLLP"
     gene            complement(3728..4642)
                     /locus_tag="PLS90_08295"
     CDS             complement(3728..4642)
                     /locus_tag="PLS90_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02444.1"
                     /translation="MAGTLDRPPPPPAESPVSDLPIMENPEWRPAPLREPTHGMRRMR
                     PDATFRTLFGLVVIWGVLVMVFVANRLSDNFLEVEPRLSQSINTDQVLMRRLDPAQKL
                     PLPLTMYMRNVSAQQAMQLVTNYVGFEMDPDDLTLLDEEPRTISFDEIPLYQAIHQLI
                     DNPGLGVSMMGRQLNLFHQRIELEGDADSGSVNWQARLPLSEARQLIVPSGQLNLWFM
                     IRLEGPPANGQGEWREVLVELWRGSDLLSLTRAPLDAQGRAQLELSASDSLVFMVKRI
                     SERAAAPGEYELAFYYLYDAEPAPAAAD"
     gene            complement(4643..5128)
                     /locus_tag="PLS90_08300"
     CDS             complement(4643..5128)
                     /locus_tag="PLS90_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HPK02445.1"
                     /translation="MTLPPNFSLVWQDRLAGSGHPGHGPTLIDTLTELRRRGFHAILS
                     LTEDAPEPALLTEHGFATLHVPIEDFTAPTPEQVRTGVRWLNEQLEHEGRALVHCQAG
                     YGRTGTILACFLVSRGLDPAAAIGEIRRTRPGSLEVYAQEFAVYQFARQLRQESGEEE
                     P"
     gene            complement(5370..5885)
                     /locus_tag="PLS90_08305"
     CDS             complement(5370..5885)
                     /locus_tag="PLS90_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPK02446.1"
                     /translation="MISQAMQDKLLEQVRVEWESEFAYLTMMAWCYNNDFDGFGAWFH
                     QQANEEREHGLRILRFINNAGGSIGIPSINVIAPDFRNLQHLFELTLQHEEQVTGSIN
                     RLVDQAMADNDHMTHTFLQWFIQEQVEEESTARHILAKIRRAEGAPGALFMIEQQLAG
                     ASPRPAAEADA"
     gene            complement(5985..6362)
                     /locus_tag="PLS90_08310"
     CDS             complement(5985..6362)
                     /locus_tag="PLS90_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HPK02447.1"
                     /translation="MTRLFIIIGSAFGLLGVAAGAFGAHGLRNILSPEMLAIFETAVR
                     YQMIHALALLAAAWLAQAAPGRAIHAAGGCFIAGILIFSGSLYLLTLSGVRAWGAVTP
                     IGGIAFLAGWALMGWHGAKRVPR"
     gene            complement(6463..7911)
                     /locus_tag="PLS90_08315"
     CDS             complement(6463..7911)
                     /locus_tag="PLS90_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02448.1"
                     /translation="MSPGHDETTRAPAGAFKPLCDRLMPPLRLRTFFQPAWLPGLASL
                     LLLGLMARVNRVHGTTGDEPHYLVMMDSLVRDGDLLILNNHEREITRDFFGEQLAPHL
                     SHAAPDRSLHFPMTALLLAPAYALDRHDGVLLLLVLLHACAVLWLRAALIHLGIAPAA
                     ASAVAFVWLLTVPWSLMALQVYPDSLGLVLVAAVLRLLVGKRKPIVRTMLTGLLLGIL
                     ASLHVKFYPLAIALLAGGIWLGGVRRAWPVMLFPFLLVVALNSILLARLYGSFWPWAP
                     YGSFASHGAGGSLLLGLPAYLADLQYGLLPQGPLWLALVPGVVVLARSRDRGPRGRFA
                     WVCLAAGLIHLLAIANTRLDLFGWSTAARNLTVAVPVGVAIAGLGLQATWRRRGFRWF
                     WALLFVWQLALLVVYQFDWWGRYKLPTLLARWLGVPRESVPSFFDYFHPDAPPDPGAW
                     VLFVAFAAVAIILGVLGWRLGRLSPEDRAALN"
     gene            8060..10444
                     /locus_tag="PLS90_08320"
     CDS             8060..10444
                     /locus_tag="PLS90_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02449.1"
                     /translation="MTMPNDRAQTPGDRAWFAGIFCVGAVALVFHAAMYSWWAFDDAF
                     ISMRYAENLVRHGALEYNLGERVEGYSNLLWTLQLALADRLGLPMFPVARTLGLLANL
                     GILLLGVYHALRFARTASPSRIARDVSACVVVLGLACAGPLAGHAQTGMETTQHALLL
                     DVAFLGGALAGRERRGWTVGAGCAMALAVFSRPEAFGYLPFFWLSLVLPESARRRLAG
                     PSAAGISPRWFVLGAALPLLALAALLAWRISYYGSPLPLTVTAKSVADAGLGGLRYIQ
                     LFFTYGPTGGVWVVLAPAVVAALCWRLPGRSENSDLCRGVPSRLLPTVLWLAGPALLL
                     FANCLFVIKVGGDILTFSRFMVPSILPVALILAYAAMRLTLVAGAVASPPAKAGCALY
                     LVLVLTLYAGQATNLFHSVEYHSRQVMTTAKAPRFIAWALKALHKGEIVTRSPYYNPY
                     SGLLVPLIQDHWMPGQSAVLGDIGMAGALAKEVRIIDANGLVTPVFARLQAERDNRAY
                     RRQALEFLTARRPEWLVLVQRAGAIELVPGLPLSEWEEASAYETLLVYRDWGERLIEP
                     APVREVVVLRRRGLAPPDTKARRQNYRRLAEVFPNSAAAAAIARNAARNADLEIPHHV
                     LEVGETAPENRIQIEGRTLVGTQMLGEFAGGGRDGWSFTGDAMTDQPFRAGAAARPTQ
                     GYVGEWLIDTSQGGPTRTAPTGRARSPEFVPRAGDYLGFRVAGAVAESSGVRLEVNGE
                     LAAHWQGVNSALLRSVLVDLTPWAGLPVRIVVEDMETRDWGWVMADHFVVYRSE"
     gene            complement(10455..>12381)
                     /locus_tag="PLS90_08325"
     CDS             complement(10455..>12381)
                     /locus_tag="PLS90_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK02450.1"
                     /translation="PFALLLSLDPGPRNRLLWRGVGCLAALLTILAIFYCRSKGEWIF
                     GILTIVIFSAIFSRIGQMPGLRKRYLLATVGGLLLLLLSIAWMLSPDLLSQLKGLDYS
                     VKSRSIIWAGAVGIFLDHPILGAGPGNFRVFFPEHRAHDYFLHEISNVTLYAHNYFLD
                     LLAETGILGFAAFVCFLLCLLGLALRWAFKHPDPRLRGLLTAMVAGIFGIFGSNLSSP
                     NGRWVIGASSLWTVLGFCCGAILQARGIHAGRLEPAEGPDPTQAPRRPRLTLAAALVA
                     VALVALPLCAYRGYVYFDSAIDYARGMRMMEPAYDMLTSGRVSDPEMVAAMLQRSATD
                     FEQSIKDNPSNVSSYYKLGSIYSTLSSLTRDEASRRNAAGQPDEARRLTEVADYYLVA
                     SKRNYAMLQQRWPDYAEIHYNLGIIYYDYAQLLDRIARGETPPRVLDSVDEGKDYRAE
                     AERYRQLALEHVQRMSELSIKPEVYRQRGQLLATMQLSERALQVYAEGAERYPEDRQL
                     LGQYLMTAEAAGDPVAMADVLEHLWRLNPSDELLLQQLLDVALQHELNDVLERTLEEM
                     ERINPIDPQLYAARAQLFRNRNEPELLMRAVERYLRVGGRDPETIQLGIETAQALNNS
                     EILDRLTQLREQMTSQP"
BASE COUNT         2154 a         3685 c         4470 g         2072 t
ORIGIN      
        1 ctggcgcgcc tgcccggcca cgggacgacg ccgattgatt tcgcggatca gacccccgag
       61 acgctgtatg ccggtgcgct gcacgagctg cgcgcgctgc gcgcggaata tgacgaggta
      121 accgtgatcg ggttttccat gggcgggacg ctggcgactt tgctggcggc ccgtgaaccg
      181 gtcgaccggc tggtgttgat cgccccctat tacggcgtga cctacgcgtg gtactacgtg
      241 ctgccgccgc acgtctggca tcagatgctg gggtggagca tgccgtggct ctaccggtgg
      301 gagtgcttca cgaagctgaa ccggccggaa gggcgcacgg gcctgtattc ctacaagtat
      361 gtgccgtcgc gcggcgttga gacgctgttc gcgctgggcg aagaggcgcg ggcggcgggg
      421 ctgctcgaag caatccagtg ccccgtcctg ctggtgatgt ccgaaggcga tcaggccagt
      481 tgcccggtcg cggcgcgcgc ggccttcgcg cgcatcggct cgcctcagaa gcaggcgcgg
      541 tggttcccgg gacgatccaa ccaccacttg ctgcgcgact gggatggcga agcggccaag
      601 ggggcgatcc tcgagtttct gggggagcgt cgcgtggacg catcttctca gccgcggggg
      661 gcggagaccg attcgatggc gtcgtccaga tcctgaagcg gtgcggtttc ggtggtcggc
      721 tggtcgtcgt cggccgcgaa cggcagcgtt gcgggcgcga cattgacatc ggtcgtgaag
      781 atatacggtt cggggaaacc ggaatcgaag acgacttcaa ggaagtaggc gcgccagccg
      841 gttggggggg cgccggggtc ggcccgatag atgccgtcgt cctgatacgg cagcgaggtt
      901 ttggtccagg ccggtccgat cgtctccagc ctgaaatcgc gcgcggcggg gttgtcggcc
      961 tgccacaggt tgacttcgag cgggtcgacg cgggtaaaaa cgcggagggc gccgcccggc
     1021 tccagttgcc agtcgaaggt gggcagcggc atgtcgttca agacggcgcg gtagaacccc
     1081 agcaggctct ccctgacatt ggtgttttgc agcgagtggt cggcgttggg gatgtagcgc
     1141 aggtatttgg gggcgcggag cctgctgaaa tagaggcgcg aggaatcagg gaggaagaac
     1201 tgatcgccgg cggcatagat caggagcttg ggcatgtcga gccggtcgcg gtaggcgaac
     1261 ggatcgacga tctggatcaa ggcgtttgtg gcgggcgttt ccatccggtc catgatgccg
     1321 gccgcctcgt agtcgcgcag cgtgggggca tagaagccgt aagcggcgtg gtgcaggcgg
     1381 atgttttcgc gcatgttgag gacgtcgatg acgagcgggg cgatgcccat gacgcgcggg
     1441 tcgacggcgg cggtcagcca ggcggtccag ccgcgcttgg aggcgccggt caggaagaaa
     1501 tcctggagcg ggaattcccc gccttcggtc gacagcatga actcctggat ggcgtccatc
     1561 gcgcggacga cgctgttgac catcgggagt tgggccggcc acgtttcgtc gccggtggcc
     1621 aggtatttgt cccaactcca ggcgatgatc gcgtcttcga ctcggtcgga tcccccttcg
     1681 ccggtgaacg acagcggctg attggggacc tgatagagca tggccgtgac ggagccggtg
     1741 gccagggcca gatcgcgcgt gagcgggtcg gcggcctcgg gcgtcgggtc gttgttcgcg
     1801 ccgccggcaa tcagcagcag ggcggtatcc tgatagaggc tgtcgggcac gttgacgagc
     1861 aaccagtgcg tccagaccgg ggcattgacc tcctggccgg aacgccaggt ctgcgaggtg
     1921 agccgcaaca gataggtttt gacgcgctgg cccttgaacg cgttggcgat ttcccacttg
     1981 taggcggggt cttttcgggc cacgtagcga tcaagcgcgg tctgacccga ggttgtcggg
     2041 ccatccgact gggcgtgggc cgcgggccag aggactgcaa aaagtgttgc tgtcaggaga
     2101 cttaccaggt atttcgtcgt ggataacatg aagcggatgc ctttccaggg agacaaggtg
     2161 gagcgatgat ccgtcagggg gcgatgtggg ggttgagcgg ggggagtccc cgcagccagc
     2221 ggcgcgcctc caggcgatag gggcgcaggg tttccgaccc gataaaacgg acgaggccgt
     2281 aaaagatcgc cagatcgtcg aggtagccca gacccacgaa caggtccttg agcaggtcga
     2341 gaggccagac gaagtagagg aaggtcagca cgatggagag cttgtggagc gaggaggcat
     2401 gctcgtccag caggtagagc cagagcgcca cgagcgtggt gacgcccggg acataggaaa
     2461 agagatagcg cagcggatgc ggcgcggggc gcggcgcgga gctgcgttgc gcggggagtg
     2521 ggccatccgc cggtgggagg atctcgacgt cgcattcccg gggcggtggg ctgggatcag
     2581 gcggagtcat ttcagggcag cagcgtccag aacgtcatcg gttcgatgtc atcgtagagc
     2641 gccaggtact tctggaccag cgagtgggag ccgaggttct tgcgctgatt cgacggcatc
     2701 gtctccacca gccgccgggc ggtgtcggct gccccttgcg ggacatccag gcggtcgaag
     2761 aaccggagca ggttgagcag gatttcgatc tggatcggtg aatcgctgag cgaggccgac
     2821 tggtagtaga acagcgcctt gtcggccagg tcgcggcggg acggatcgtc gatgaactcc
     2881 agttgatacc gcgcgcccag gcagttgtag aggtaggccg tcagcggcga agcgggggcg
     2941 gtgcggacga tttcctcggc ttcctcaatc gacatctcgg gggggagccg gcccaactgc
     3001 atgctgatga aacccttgat ttgtttcagg cgctcgatca gcggcttcag ctcgtcaggg
     3061 gtttcgcgga tccgcaaggt gaattcgggg taattcagtt ggatgttccg cagggcggcg
     3121 cccccctcct tgaccgacgc ggtctggacg atgcgaatcc tgtattcgcc gggggcttcg
     3181 aaggccagtc cgacgctcga ttcgcgatcc gcccagaggt ggacctgcgt cgtcgagctg
     3241 gcgagcgggc ggagcgggat ttcaaccgtg ccgtagagtt caggcagatg cgcgcccgga
     3301 tagcgttcct cgcgatcatt ttcgggctgg atgtagacct gaagctgcga gaccgggacg
     3361 aaattggtct cgatcgtcac cgggaagcgg gtgtgattgc tgaggacgag cggcacggcg
     3421 accggctcgc cgacgaagta gctgcgcttg atgcgcccaa ggtcgacggt cgagcgcaga
     3481 aaatccgtct catcgaaagg gtagaactgt tgacgcgtga ggacgggcac gccggtttgc
     3541 tgggcaggcg ccgggaacaa ggcgaggcag aagactcctg cgctcaggat caggcggaat
     3601 gaacggcgga cagacatgga acgaggaacc cttccgtaac gaattgcgaa gcgggcaaat
     3661 tcctggagcg gcgccggcgg gctggtctcg gctgcgacac gagattggac ccatggcgcc
     3721 ggccgggtca atcggcggcc gccggggccg gttcggcgtc atagagataa tagaaggcca
     3781 gctcatactc gccgggggcg gcggcgcgct cgctgatcct ttttaccatg aacaccaggc
     3841 tgtcgctggc gcttaattcg agctgggccc ggccctgcgc gtcgagcggc gcgcgggtga
     3901 gcgacagcag atccgaaccg cgccagagtt cgaccagcac ctcgcgccat tccccctggc
     3961 cgttggcggg cgggccctcc agccggatca tgaaccagag gttcaactgg ccgctgggga
     4021 cgatgagctg gcgcgcctcg gagaggggga gccgcgcctg ccagttgacg ctgccgctgt
     4081 cggcatcccc ttccagttcg atgcgttgat gaaacagatt gagctggcgc cccatcatcg
     4141 aaacgccgag acccgggttg tcgatcagtt ggtggatcgc ctgatagagc gggatttcat
     4201 cgaagctgat cgtccgcggc tcctcatcca gcagcgtcag atcgtccggg tccatttcaa
     4261 aaccgacgta attggtgacc aactgcatcg cctgttgcgc cgaaacgttg cgcatataca
     4321 tcgtcagcgg cagcgggagt ttctgggcgg gatcgagtcg gcgcatcagg acctgatcgg
     4381 tgttgatgct ctggctcagg cggggctcca cttccaggaa attatccgag aggcggttgg
     4441 cgacgaagac catcaccagc acgccccaga tgaccaccag gccgaagagg gtgcggaagg
     4501 tggcgtcggg gcgcatgcga cgcatcccgt gggtgggttc gcgcagcggg gccgggcgcc
     4561 attccgggtt ttccatgatc ggcaggtccg ataccgggct ttccgccggc gggggagggg
     4621 ggcgatcgag cgtcccggcc acctagggct cctcctctcc ggattcctgg cgaagctggc
     4681 gggcgaactg gtagacggcg aactcctgcg catagacctc gagcgaacca gggcgcgtcc
     4741 ggcggatctc gccaatggcc gcggcggggt ccaacccgcg cgagaccagg aagcaggcca
     4801 gtatcgtccc ggtgcgcccg tagcccgctt gacaatggac caacgcgcgg ccctcatgtt
     4861 ccagttgttc gttgagccag cggacgccgg tccgcacctg ctcgggggtg ggggcggtga
     4921 aatcctcaat cggtacatgc aacgtggcga agccgtgttc ggtcaggagc gccggttcgg
     4981 gggcgtcctc ggtcaggctg aggatcgcgt gaaagccgcg gcggcggagt tcggtcagcg
     5041 tgtcgatcag cgtgggtccg tgaccgggat ggccggagcc ggccagtcgg tcctgccaga
     5101 cgaggctgaa gttgggcgga agggtcatgc ggagggcggc cgcctgcaaa gggcgatctc
     5161 ctttagtatc agtcttcgag tatcctgcaa gctcgcacgg ggacacaaag gatttctgcc
     5221 ggaggaaatg agcggggaga aggaggggag caagggaagg ggaggcaagc ggcggcctgg
     5281 acgccggttt cgagagccga agaagagcga cgagcgacga tggttgggag atagaggagt
     5341 gccgaatggt tttcgacgag atcggtggct caagcgtcgg cttcggctgc ggggcgcggg
     5401 ctggcgccgg ccagttgctg ctcgatcatg aacagggcgc ccggggcccc ctcggcccga
     5461 cggattttcg ccagaatgtg acgcgcggtc gattcctcct cgacctgttc ctgaatgaac
     5521 cattgcagga aggtgtgcgt catgtggtcg ttatcggcca tggcctgatc caccagccga
     5581 ttgatgctgc cggtgacctg ttcctcgtgc tgcaacgtca gttcgaacag gtgctgcagg
     5641 ttgcggaaat cgggggcgat cacgttgatc gacgggatgc caatgctgcc gcccgcattg
     5701 ttgataaacc gcaggatgcg caggccatgt tcgcgttctt cattggcctg ctggtgaaac
     5761 caggcgccga agccgtcgaa atcgttgttg tagcaccacg ccatcatcgt cagataggcg
     5821 aactccgatt cccattcgac ccgtacctgc tccaacagct tgtcctgcat ggcctggctg
     5881 atcatggaag cggcccgcct ttcgttccgg gtaagtgaat gtgcgggtca ttgtggctgg
     5941 aaacggcaac ggcttcaatc agaaagcagg cgcgacggtc ggcgctagcg gggcacgcgt
     6001 ttggcgccgt gccagcccat gagcgcccaa ccggccagga aggcgatgcc gccgatcggg
     6061 gtgacggcgc cccaggcgcg gacgccgctg agcgtcagga gatagagact gcccgagaag
     6121 atgaggattc cggcgatgaa gcacccgccg gcggcgtgga tggcacggcc cggcgccgcc
     6181 tgcgccagcc atgcggcggc cagcagcgcc aacgcatgga tcatctggta gcggacggcc
     6241 gtttcgaaga tggcgagcat ttcgggggaa aggatattgc gcagtccatg cgcgccgaac
     6301 gcccccgccg ccacgccgag caacccgaac gccgagccga tgatgatgaa aaggcgagtc
     6361 atgttgcctt ccgagctgga gtgtgaacgc aaggacttgg atagaggggg catttccatg
     6421 gcgcgccggg aggagccacg tgcttcgctc tctgcttcgg tcctagttca gagcggcccg
     6481 gtcctcaggc gaaaggcggc cgagcctcca accgagcacc ccaaggataa tcgcgacagc
     6541 cgcgaaagcc acaaaaagca cccatgcccc cggatcgggc ggggcgtcgg gatggaagta
     6601 atcgaagaag ctcggcacgg actcgcgagg gacgccaagc caccgcgcca gaagcgtcgg
     6661 cagcttgtag cggccccacc agtcgaattg gtagaccacc agcagcgcaa gctgccagac
     6721 aaacagcagc gcccagaacc agcggaagcc gcgccggcgc caggtagctt ggagcccgag
     6781 ccccgcgatg gcgacgccca ccggcacggc gacggtcagg ttgcgggcgg cggtgctcca
     6841 gccgaagagg tcgagccgcg tgttggcgat cgcaagcagg tgaatcaggc cggctgccag
     6901 gcagacccac gcaaaccggc cgcgtgggcc tcggtctcgg cttcgggcga gaacgacaac
     6961 gcccggaaca agcgccagcc agagcggccc ctgtggcagc agaccgtact ggaggtccgc
     7021 gaggtaggcg ggcaaaccga gcaggaggct gccgcccgcg ccgtgcgacg cgaaggagcc
     7081 atagggcgcc cacggccaga agctgccgta gagccgcgcc agcaggatgg agttcagggc
     7141 gacgaccagc aggaagggga acagcatgac aggccatgcc cggcgcacgc cgccaagcca
     7201 gatgccgccg gccagcagcg cgatggccag cgggtagaat ttgacatgca gcgaggcgag
     7261 gatgccgagc aagaggccgg tcagcatcgt ccggacgatc ggtttgcgct tgccgaccag
     7321 aaggcgcagg acggccgcaa ccagcacgag ccccagtgaa tcgggataga cctgcagggc
     7381 catcaggctc cacgggaccg tcagtagcca gacgaaggca acggcgcttg cggcggccgg
     7441 cgcgatgccg agatggatca gcgccgcgcg aagccacagg acggcgcacg catggagcag
     7501 gacgaggagc aggaggacgc cgtcgtgccg gtcgagggcg taggccggcg ccagcagcag
     7561 cgccgtcatc gggaagtgca gcgagcggtc gggggcggcg tgggagagat ggggcgcaag
     7621 ttgctcgccg aagaaatcgc gcgtaatctc gcgctcgtgg ttgttgagga tcagcagatc
     7681 cccgtcgcgc accagcgaat ccatcatgac gagataatgc ggctcgtcgc ccgtcgtgcc
     7741 atggacgcgg ttgacgcgcg ccatcagtcc gagcaggagg agggacgcca ggccggggag
     7801 ccaggctggc tggaagaaag tgcgcaggcg gagcgggggc atcagccgat cacataaggg
     7861 tttgaacgcg ccggccggcg cgcgggtcgt ttcatcatga ccggggctca tctcactttc
     7921 cagatggatg gattcgacac ggtcgcccgg tgtcgcagcc aggggacgaa tcgattgacc
     7981 attgcccgcc gccgtgcgac aagttcaccg tccgaaccga atgccgccgc gcagcaaccg
     8041 ggaatgccgt gatccgccgt tgacgatgcc gaacgaccgc gcccagaccc caggcgatcg
     8101 cgcctggttt gccggcattt tctgcgtcgg cgccgtcgcg ctcgtcttcc atgccgcgat
     8161 gtacagctgg tgggcgttcg acgatgcgtt catctcgatg cgctatgcgg aaaacctggt
     8221 tcgccacggc gcgctcgaat acaacctggg cgagcgggtc gaaggctact ccaacctgct
     8281 gtggacgctg cagctcgcac tggccgaccg gctgggcctg ccgatgtttc cagttgcgcg
     8341 gacgctgggc ctgctcgcca atctcgggat tctgctgctg ggcgtctacc atgcgttgcg
     8401 gtttgcgcgc acggcttcgc ccagcagaat cgcgcgcgat gtcagcgcct gcgtcgtcgt
     8461 gctcggcctt gcctgcgccg gcccgctggc cggccacgcg cagaccggga tggagacgac
     8521 gcagcacgcc ctgctgctcg atgttgcgtt tctggggggc gcgctggccg gccgggagcg
     8581 gcgcggctgg accgtcggcg cggggtgcgc catggcgctc gctgtattca gccggcccga
     8641 agcgttcgga tacctgccgt tcttctggct gtcgctggtc ctgcccgaat ccgcgcgacg
     8701 aagacttgcc ggtccatcgg cggcggggat ttcgccgcgg tggttcgtcc tcggcgcggc
     8761 actgccgctt ttggcgctgg cggccttgct cgcatggcgc atcagctatt atggctcgcc
     8821 gttgccgctg accgtcaccg ccaagtccgt tgccgacgcc ggcttgggcg ggctccgata
     8881 cattcaactc ttcttcacgt acgggccgac gggtggcgtg tgggtcgtgc tggcgccggc
     8941 cgtcgtcgcg gcgctgtgct ggcgtctgcc cggccggagc gaaaattcgg acctttgtcg
     9001 cggagttcct tcgcgtctgc tgcccaccgt cctctggctg gcggggccgg cgctgctcct
     9061 ctttgccaac tgcctgttcg taatcaaggt cggcggcgac atcctcacgt tttcgcggtt
     9121 catggtcccc tcgattctgc cagtggcgct gatcctcgcc tatgcggcga tgcggttgac
     9181 gctcgttgcc ggcgccgtgg catcgccgcc cgcgaaggcc ggctgcgctt tgtacctggt
     9241 tcttgtcctg acgctctatg cgggtcaggc gaccaatctg tttcattccg tcgagtatca
     9301 ctcgcggcag gtgatgacga cggcgaaggc gccgcgtttc atcgcctggg cgctcaaggc
     9361 cctgcacaag ggggagatcg tcacgcgctc gccgtactac aatccctact ccggcctgct
     9421 ggtgccgctg attcaggatc actggatgcc gggccagagc gccgtcctcg gcgacatcgg
     9481 catggcgggc gcgctggcca aggaagtccg gatcatcgat gcgaacggcc tggtgacgcc
     9541 ggtcttcgcc cgcctgcagg cggaacgcga caaccgggcg tatcgccgcc aggcgctgga
     9601 gttcctgacg gcccggcggc ccgaatggct ggtgctggtc caacgcgcgg gcgcgatcga
     9661 gctcgtgccg ggcctgccgc tgagtgaatg ggaggaggct tcggcctacg agacgttgct
     9721 cgtctatcgg gactgggggg agcgactgat cgagccggcg ccggtgaggg aggtggttgt
     9781 gctacggcgg aggggactgg cgccgccgga tacgaaggcg agacggcaaa actatcgtcg
     9841 gctggccgag gtttttccga attcagcggc ggcggcggcg atcgcgcgca atgccgcgcg
     9901 caacgcggac ctcgagattc cgcatcatgt gttggaggtg ggagaaaccg cgccggaaaa
     9961 ccgaatccag atcgagggtc gcacgctggt gggaacacaa atgctgggtg agttcgcggg
    10021 cggcgggcgc gacggctgga gcttcacggg cgacgcgatg acggaccagc cgtttcgcgc
    10081 cggggcggcg gcgcggccga cgcagggcta cgtcggcgag tggctgatcg atacatcgca
    10141 gggcgggcca acgcggaccg cgccgaccgg tcgcgcccgc tcaccggagt tcgtcccccg
    10201 cgccggcgat tacctcggct ttcgggtggc gggcgccgtg gcggagtcca gcggcgtgcg
    10261 cctggaggtg aacggcgaac tcgcggccca ttggcagggg gtcaactccg cgttgctgcg
    10321 ttccgtgctg gtggatctga cgccgtgggc cggcctgccc gtgcggatag tcgtcgagga
    10381 catggagacg agggattggg gctgggtcat ggccgaccat ttcgtcgtgt atcggtcgga
    10441 atgaggtgcg gcgctcacgg ttggctggtc atttgctcgc gcagttgggt gaggcggtcg
    10501 aggatttcgc tgttgttcag agcctgcgcc gtctcgatgc caagctggat cgtttccgga
    10561 tcgcgcccgc ccacgcgcag ataacgttcg acggcgcgca tcagcagttc gggttcattg
    10621 cgattgcgaa acaactgcgc ccgggccgcg tagagctggg gatcgatagg gttgatccgc
    10681 tccatctctt cgagcgtccg ttcgagcacg tcgttgagtt catgctgcag cgccacatcc
    10741 agcagctgct ggagcagcag ttcgtcgctg gggttcaggc gccagaggtg ttcgaggacg
    10801 tcggccatgg cgaccggatc gcccgccgcc tcggccgtca tcagatactg gccgagcagt
    10861 tggcggtcct ccggatagcg ctcggcgccc tcggcataga cttgcagcgc gcgctcggaa
    10921 agctgcatgg tcgccagcaa ctggccgcgc tggcggtaga cctccggctt gatggacagc
    10981 tcgctcatcc gctggacatg ttcgagggcc aactggcggt agcgttcggc ctcggcccga
    11041 taatccttcc cctcgtcgac tgagtcaagg acgcgggggg gcgtctcgcc gcgcgcaatc
    11101 cgatcgagca actgcgcata gtcgtaatag ataattccca ggttgtagtg gatctcggcg
    11161 tagtccggcc agcgctgctg gagcatggcg tagttgcgct tggaggccac caggtagtag
    11221 tcggcgactt cggtcaggcg gcgggcttcg tcgggctggc cggcggcgtt gcgccgtgag
    11281 gcttcgtcgc gggtcaggct ggacagcgtc gaatagatcg atccgagttt gtaatacgag
    11341 gagacgttgc tcggattatc cttgatcgac tgctcgaaat cggtggcgct gcgttgcagc
    11401 atcgccgcca ccatctccgg gtcggagacg cgccccgacg tcagcatgtc gtaggccggc
    11461 tccatcatgc gcatgccccg ggcataatcg atcgccgaat caaagtagac gtaaccccgg
    11521 taggcgcaaa gcggcaaagc gaccagcgcc accgcgacca gcgccgccgc cagggtcagg
    11581 cggggccgcc gcggggcctg cgtcgggtcc ggcccctcgg ccggttcgag ccggccggcg
    11641 tggattcccc gcgcctgcag aatcgcgccg cagcagaacc ccagcaccgt ccacagggac
    11701 gacgcgccga tgacccagcg cccgttgggg ctgctcaggt tgctgccgaa gatcccgaag
    11761 atgccggcga ccatcgccgt cagcaggccg cgcaggcgcg ggtcgggatg cttgaaggcc
    11821 cagcggaggg ccaaccccag caggcagagg agaaagcaga caaacgcggc gaaaccgaga
    11881 atgccggttt cggcgagcag atcgagaaag taattgtgag cgtagagcgt gacgtttgag
    11941 atctcgtgga ggaagtaatc atgcgcgcgg tgctcgggga agaacacgcg gaaattgccc
    12001 ggccccgctc cgagaatcgg gtggtcgagg aagatgccga ccgcccccgc ccagatgatg
    12061 ctgcgcgatt tgacggagta atccaacccc ttgagctggc tgagcagatc gggcgagagc
    12121 atccaggcga tcgacagcag gagcagtagc agtccgccga cggtggccag tagatagcgc
    12181 ttgcgcaggc cgggcatctg gccgatgcgc gagaagatcg cggagaagat cacgatggtg
    12241 aggattccga agatccactc gcccttgctg cggcaataga agatggccag gatcgtcagc
    12301 aaggcggcca ggcagccgac gccgcgccac aacaggcggt tgcgcggacc ggggtccagg
    12361 ctcaacagca gggcgaacgg c
//
LOCUS       DAOPCO010000096        12371 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5067_length_12371_cov_4.749838, whole genome shotgun sequence.
ACCESSION   DAOPCO010000096 DAOPCO010000000
VERSION     DAOPCO010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12371
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5067_length_12371_cov_4.749838"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="PLS90_08330"
     CDS             <1..1846
                     /locus_tag="PLS90_08330"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011129353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HPK02451.1"
                     /translation="NSLLWAIGEDIPYIPNKRRGGVVIGGSIAPIFFNTLEDSGALPI
                     QCDVSNLRTGQRIRVLPRAGKITDPEGNTLATFTLQPNTLPDEYRAGGRVPLIIGRQL
                     TNNVRAELGLGESDVFTHPRFVVEEGVGFTLAQKMVGRACGCGVEGVHPGTSCTPKMT
                     TVGSQDTTGPMTRDEMIELACLGFSADLVLQSFCHTAAYPKPADKQTHATLPRFIIER
                     GGISLRPGDGIIHSWLNRMLLPDTVGTGADSHTRFPIGISFPAGSGLVAFAAALGVMP
                     LEMPESVLVRFSGRLRPGITLRDVVNAIPFYAVQAGLLTVPARNKKNVFNGRILEIEG
                     LPDLKVEQAFEISDASAERSAAGCTIELGEEPIKEYLRSNITLLQAMIHDGYQSVKTI
                     ERRIRKMEEWMANPKLLRRDPNARFAAVLEIDLDDIREPLVACPNNPDDVRPISEVAG
                     DPVQEVFIGSCMTNIGHYRAAAKVLEGYTELPVRLWVCPPTRMDEEQLKAEGVYATFE
                     NIKDARTELPGCSLCMGNQARLNPGSTCMSTSTRNFEGRMGDNTRVYLGSAEISAVTA
                     ILGRVPTPAEYMEAVTTRIDPLSAELYRYLNFDQIKEYRERAQLAGVS"
     assembly_gap    1922..2021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2348..2424
                     /locus_tag="PLS90_08335"
     tRNA            2348..2424
                     /locus_tag="PLS90_08335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Val,seq:gac)
     gene            complement(2573..3502)
                     /locus_tag="PLS90_08340"
     CDS             complement(2573..3502)
                     /locus_tag="PLS90_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HPK02452.1"
                     /translation="MARPMSKIDPAWWDYTTLAPEILRDAAGLTERDLGELQRPGFTI
                     QFYDSLEELFVAEAMEYIEAWRQSTADNPTGICGPIGPTEQLPLVARIVNSLGLNLMD
                     AHFWGMDEWVEAGRAVTAAHPLSFQRADRELCFDRIDARLRMPEANLHFPVGDLAEYT
                     ASFDQVRCVIMQGGQGEVKHWAFNDPPRREGEFRDAPPPAERYRQLSARVTELHPLTL
                     IQNARTSAGGYLPQVPTQAVTVGPVETWKAERVSIWHPGHHDNAFGMRLTAFMISKRI
                     VDSAVPMSLLAEHPDVRFHYYRGGLGEVTVEMH"
     gene            complement(3483..4274)
                     /locus_tag="PLS90_08345"
     CDS             complement(3483..4274)
                     /locus_tag="PLS90_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007327666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPK02453.1"
                     /translation="MAHPDDVEFVMAGTLILLAEAGYEPHVMNIADGSLGSTSAGAEE
                     TAGRRLAEARAAAESIGAIHHPPIARDLELFYCDRLLRRLAAVVRRINPTIVLVQAPF
                     DYMEDHMNASRLAVSATFSKGMPNYRTDPPVDAAGGPCAVYHAMPHGLRDPLRRRVEP
                     EFLVDITRCIGRKAEMLACHHSQREFLTQSQAMDSFVETMRAMAAELANRYGLAGYAE
                     GWRRRLHLGFGEEDFDPLRDALGESIYECEPNDSGKEQGDGSSDE"
     gene            4688..6688
                     /locus_tag="PLS90_08350"
     CDS             4688..6688
                     /locus_tag="PLS90_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HPK02454.1"
                     /translation="MSDRDGRKRVIIERVSPEIDCGQYAAKITVGERLTVEADVFCDG
                     HDRPWAALLFRREGSPRWSEIPMQPLPNDRWRASFLVTDQGRYEYSVLAGVDHFGTWH
                     HDLRKKIHAGVDVAVDLLIGGRLIQRAAERAERDGGGDGRQLAEWAALLGSDAPVAEK
                     LSLIQDGRLLSLLACHRDRDLALRYPRVLRLIAERERARFSTWYELFPRSTSGDESRH
                     GTFRDVERRLPAIAGMGFDILYLPPIHPIGLTKRKGKNNALVAGPDDPGSPWAIGGPE
                     GGHTAVHPQLGTLADFRRLVRAAADLGIEIALDIALQCSPDHPYVRDHPEWFTRRPDG
                     AVQYAENPPKKYEDIYPFNFECDDWRALWAEILGIFQHWIEHGVRVFRVDNPHTKPFR
                     MWDWLIEQIHRDHPDVLFLAEAFTRPKVMYRLAKGGFTQSYTYFAWRNEKWDLTEYLR
                     ELTATEARDFFRPNFWPNTPDILTEFLQRNGRAGFLQRLVLAATLAASYGIYGPAFEL
                     MESTPRHEGSEEYLNSEKYQLRAWDLDRPDSLSDFIARVNQIRRENPALQSNRNLAFH
                     TINNDQLIAYSKATEDRANVILCVVNLDPHHTQSGWIALDLNALGLAGDQPFQVHDLL
                     SNARFQWTGARTFVELDPRVVPAHIFRIRRRLRSERDFDYYL"
     gene            6707..10084
                     /gene="treS"
                     /locus_tag="PLS90_08355"
     CDS             6707..10084
                     /gene="treS"
                     /locus_tag="PLS90_08355"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HPK02455.1"
                     /translation="MARTRKPPIELDHDPQWYKDAIIYELHVRAFHDSDGDGYGDFRG
                     LAQKLDYVSDLGVTAIWLLPFYPSPLRDDGYDIANYMDVHPRYGNLRDFKTLLAEAEK
                     RGIRIISELVLNHTSDLHPWFQRARQAPPGSPHRDWYVWEQNPDRYQEARIIFQDYEH
                     SNWTWDHVANAYYWHRFYSHQPDLNFDNPAVRRELIKVLDYWFAMGVSGMRLDAVPYL
                     YEQDGTNCENLPRTHEFLKELRRHLDRKFDGRMLLAEANQWPEDSREYFGDGDECHMA
                     FHFPLMPRMFMAVQLEDRFPIIDILDQTPAIPDNAQWALFLRNHDELTLEMVTDEERD
                     YMYRAYASDLQARVNLGIRRRLAPLLGNNRRKIELMNGLLFSLPGTPVLYYGDEIGMG
                     DNIYLGDRDSVRTPMQWSADRNAGFSRANPQQLYLPVITDYEYRYETINVETQRNNPH
                     SLLWWMKRLIALRKNYRAFSRGTMDILHPDNRKVLAFIRRHDEEIVLVVANLSRFVQC
                     LELDLKSFEGMTPVELFGQNPFPRIGELPYFITLGPHSFYWFALERSDADARRQSLEE
                     RPLPQLTVAGAWDHLFRPERRAAFERILPEYLALARWFGGKARKIKSTCIVETIPVPI
                     RPHHALLVMLEVEYTDGDPETYLLALAWADGERAQQLQTASRHLILARLDVGARGGEP
                     RAGVLYDAFCERAFNAALLTVLARRRKLVGLQGHVLGLNTPQLARLLAGDSPDNLESH
                     VIRGEQSNTSVIYGERFILKLFRRIERGINPDIELTRCLTARRFAPIAPLAGWFEYVQ
                     GRREPIQLGLLQAFVVNEGDAWQYTLDAMGWFMEQVLSHSGRPLPPAATGGLVELSLR
                     EPPEEARESIGHYLEMARLLGERTAQMHQALAADPADPAFKPESFSQLYQRSLYQSMR
                     NLIGQTFQLLDRQLRALPESTRDPARDLLARQDELLARLRAVLQRRITAKRIRTHGDY
                     HLGQVLYTGKDFVILDFEGEPSRSLTERRLKRSALRDVAGMLRSFHYAAYTVCRNLLR
                     DGMIHEEDRPRMQQWARFWYRWVAAAFLAGYLPIGQGAGFLPRTHDELQLLIDAYMID
                     KCVYELKYELNNRPDWVEIPLKGLLNLLESTLAPHVE"
     gene            10104..12041
                     /gene="glgB"
                     /locus_tag="PLS90_08360"
     CDS             10104..12041
                     /gene="glgB"
                     /locus_tag="PLS90_08360"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015183599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HPK02456.1"
                     /translation="MTQTGMSVPPVLYDNSLLTDDDLHWFNEGTHYRLYQKLGSHVVE
                     RDGRSGVNFAVWAPNAEAVTVIGDFNGWNKESHPLRPRGQSGVWEGFIPGLAKGTHYK
                     YHVRSRFNLYRADKADPYAVHHETPPHTGSIVWDLDYEWRDADWMAHRHARNSLNSPI
                     AIYEVHLGSWRRNPAENHRPLNYRELAPLLADYVADSGFTHVEFLPIMEHPFYGSWGY
                     QTTGYFAPTSRYGTPQDFMFLIDYLHQHGIGVILDWVPSHFPTDEHGLGYFDGTHLYE
                     HADPRQGFHQDWASLIFNYGRNEVRNFLMSSALYWLDRYHVDGLRVDAVASMLYLDYS
                     RKAGEWVPNAFGGRENLEAIDFLRRFNTEVFQHFPDVQTFAEESTAWPMVSRPTYVGG
                     LGFGLKWDMGWMHDTLSYFQKDPIHRKYHHGQITFRMLYAFNENFILPLSHDEVVHGK
                     RSLLDKMPGDRWRKFANLRLLFSYMYAQPAKKLLFMGGEFGQWREWSHDESLDWHLFL
                     EPDHKGLHNLVRQLNRIYREEPALHHFDLSSDGFEWIDCNNTEDSTVILLRRGPGGDR
                     DVIIAAFNFTPVVRYNYRVGVVHKGLWREILNSDALEFGGSGVGNMGAVHSDPIPFHG
                     RPQSINITLPPLGAVFFRHEG"
     gene            12031..>12371
                     /locus_tag="PLS90_08365"
     CDS             12031..>12371
                     /locus_tag="PLS90_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02457.1"
                     /translation="MRADYADFLSGRRLGATPGATGRARFSVWAPAARRVELHLHGAR
                     EEFLDLSSGGDGLFSREVPDVPPGALYTYRLDGRREFHGNFRFESFNRLFHAGDRVVG
                     GLDLGGVDATI"
BASE COUNT         2140 a         4852 c         3248 g         2031 t
ORIGIN      
        1 caactccctc ctctgggcca tcggcgagga catcccctac atcccgaaca agcgccgcgg
       61 cggcgtcgtc attggcggca gcatcgcccc cattttcttc aatacgctcg aggattcggg
      121 tgcgctcccc atccagtgcg atgtctccaa cctgcgcacc ggtcagcgga tccgcgtcct
      181 gccccgcgcc ggaaagatca ccgaccccga gggcaacacg ctggcgacct tcaccctcca
      241 gcccaacacg ctccccgatg aataccgcgc cggcggccgc gtgccgctga tcatcggccg
      301 ccaactgacc aacaacgtgc gcgccgagct cggcctgggc gaaagcgacg tcttcaccca
      361 cccccgcttc gtcgttgagg aaggcgtcgg tttcaccctc gcccagaaaa tggtcggccg
      421 cgcctgcggc tgcggcgtcg agggcgtcca ccccggcacg agctgcaccc ccaaaatgac
      481 gaccgtcggc agccaggaca ccaccggccc gatgacccgc gacgaaatga tcgagctcgc
      541 ctgcctcggc ttcagcgccg acctcgtcct ccaatcgttc tgccacacgg ccgcctaccc
      601 caagccggcc gacaagcaga cgcacgcgac cctcccccgc ttcatcatcg agcgcggcgg
      661 catctcgctc cgccccggcg acggcatcat ccatagctgg ctcaaccgca tgctgctgcc
      721 cgacaccgtc ggcaccggcg ccgacagcca cacccgcttc cccatcggca tcagcttccc
      781 ggcgggctcg ggactcgtcg cgttcgccgc cgccctcggc gtcatgcccc tcgaaatgcc
      841 cgaatcggtc ctcgtccgct tcagcggccg gctgcgcccc ggcatcacgc tgcgcgacgt
      901 cgtcaacgcg atcccgttct acgcggtcca ggcgggcctg ttgaccgtcc cggccaggaa
      961 caagaagaac gtcttcaacg gccgcatcct ggagatcgag ggcctgcccg acctgaaagt
     1021 cgagcaggcg ttcgagatct ccgacgcctc ggccgaacgc tccgccgccg gctgcacgat
     1081 cgagctgggc gaggagccga tcaaggaata cctgcgctcc aacatcacgc tcctccaggc
     1141 gatgatccac gacggctatc agtccgtcaa aaccatcgag cgccgcatcc gcaagatgga
     1201 ggagtggatg gccaacccca agctgctgcg gcgcgacccc aacgcccgct tcgccgccgt
     1261 cctcgaaatc gacctcgacg acatccgcga gccgctcgtc gcctgcccca ataaccccga
     1321 cgacgtccgc ccgatcagcg aagtggccgg cgaccccgtc caggaggtct tcatcggctc
     1381 ctgcatgacc aacatcggcc actaccgcgc cgcggccaag gtgctcgagg gctacaccga
     1441 actcccggtc cgactgtggg tctgcccgcc cacccggatg gacgaggaac agctcaaggc
     1501 cgagggggtc tacgccacgt tcgagaacat caaggacgcg cgcaccgaac tgcccggctg
     1561 cagcctctgc atgggcaacc aggcccggct caaccccggc tcgacctgca tgtcgacctc
     1621 cacgcgcaat ttcgagggac gcatgggcga caacacccgc gtctacctcg gcagcgccga
     1681 aatctccgcc gtcacggcca tcctcggccg cgtgccgacc cccgccgaat acatggaggc
     1741 ggtcaccacc cgcatcgacc ccctctccgc cgagctctac cgctatctga acttcgatca
     1801 gatcaaggaa taccgcgaac gcgcccaact ggccggcgtc tcgtaaacta caactccggg
     1861 aggaccagcc cacgaccgac cgatgcctta ccggccctcg tgctcgcccc catcggcctt
     1921 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccaaaatt cggctcccgg
     2041 ctctcgtccc tcgtcctcgg ctctcgtcac cgaaccctct gccatgtcaa ccagcgcctg
     2101 ggaccgccgg catccttgcc ggcacgtctg ggtccgcccc ccatcggcct tccccaaaat
     2161 tcggctcccg gctctcgtcc ctcgtcctcg gctctcgtca ccgaaccatc tgccatgtca
     2221 accggcgcct gggaccgccg gtgatccctt tccccttccg cggccggcct ccacagcccc
     2281 gcaacctcgc agaaccgcct tgaaatggcc cgcccgtttc gctagcgtat gggcgtcctc
     2341 agcgcgtggg tggttagctc agctggctag agcgctgcgt tgacatcgca gaggtcagag
     2401 gttcaagtcc tctaccaccc accatcgcaa aacaccgaac gcagccgccc ccgccgggcg
     2461 gcgtttttgt tcccgccgcg catccgccga atcccgaatc acaccagacg ccgcgtatcg
     2521 ctcagtcgct cggacgagaa tcgcgagcca ggagacggct cttccttccc gctcaatgca
     2581 tctccaccgt cacctcgccc agcccgccgc ggtaataatg aaacctcacg tccggatgct
     2641 cggccagcag cgacatcggc acggcgctgt ccacgattcg cttcgatatc atgaacgccg
     2701 tcagccgcat cccgaacgcg ttgtcgtgat gccccggatg ccagatgctg acccgctcgg
     2761 ccttccacgt ttcgaccgga ccgaccgtca cggcctgcgt cggcacctgg ggcaggtagc
     2821 ccccggccga agtccgcgcg ttctggatca gcgtcagcgg gtgcagctcc gtcacgcgcg
     2881 cgctcagttg ccgatacctt tccgccggcg gcggggcgtc gcgaaattcc ccctcgcgcc
     2941 gcggcggatc gttgaacgcc caatgcttca cctccccctg gcccccctgc atgatcacgc
     3001 accgcacctg atcaaagctc gccgtatact ccgccaggtc gccgaccggg aaatgcaggt
     3061 tcgcctcggg catgcgcagc cgcgcgtcga tccggtcgaa gcacagttcg cgatcggcgc
     3121 gctggaacga cagcggatgc gccgccgtca cggcccgccc cgcctcgacc cattcatcca
     3181 tcccccagaa gtgcgcgtcc atcaggttca gccccaggct gttgacgatc cgcgcgacga
     3241 gcggcagttg ctccgtcggc ccgatcgggc cgcagatccc cgtcgggttg tcggccgtcg
     3301 attgccgcca cgcctcgatg tattccatcg cctcggcgac gaacagctcc tccagcgagt
     3361 cataaaactg gatcgtaaac ccgggccgct gcagctcgcc cagatcccgc tcggtcagcc
     3421 ccgccgcatc ccgcaggatc tcgggcgcca gtgttgtgta atcccaccag gccggatcga
     3481 tcttactcat cggacgagcc atcgccctgc tccttgcctg aatcgtttgg ttcgcattcg
     3541 taaatggatt cccccagtgc gtcgcgcagc gggtcgaagt cctcctcgcc gaatcccaga
     3601 tgcagccgcc tgcgccaacc ttccgcatac cccgccagcc cataccggtt cgccagttcg
     3661 gccgccatcg cgcgcatcgt ctcgacgaac gaatccatcg cctgcgactg cgtcagaaac
     3721 tcccgctgac tgtgatgaca cgcgagcatc tccgccttcc gaccgatgca ccgcgtgata
     3781 tcgaccagga actcgggctc cacccggcgg cgcagcggat cgcgcaggcc gtgcggcatc
     3841 gcgtggtaga ccgcgcacgg cccgccggcc gcgtccaccg gcggatcggt tcggtagttc
     3901 ggcatcccct tgctgaacgt cgcgctgacc gccagccggc tggcgttcat gtgatcttcc
     3961 atgtaatcga acggcgcctg aaccagcacg atcgtcggat tgatccggcg gacgacggcc
     4021 gccaaccggc ggagcagtcg gtcgcaataa aatagctcca ggtcgcgcgc gatgggcgga
     4081 tggtggatcg cgccgatcga ttcggccgcc gcccgggcct cggccagccg ccgcccggcg
     4141 gtctcctccg ccccggccga tgtgctgccc agactcccgt ccgcgatgtt catcacgtgc
     4201 ggttcgtagc cggcctccgc cagcagaatc agcgtgccgg ccatcacgaa ctcaacatcg
     4261 tccggatgcg ccacgcacgc caagactcgc ggcctgtcgc atggctcgcc cgggggattc
     4321 atgacggatc ctccaccgca accttgatcc caatccaatt ttggagggga attctgactg
     4381 ttcggcagcg ctctggcaac agaaccgtat cacgccgccc acgcaccctc aagaatctcc
     4441 cgtccgctca tctcccgcgg gaactcgatt ttccagcccc ctcgcgctcc atgcggcttg
     4501 gatcttgctc ctgaacgatc gccatcaaaa ccatgcttcc ccggctctcc cggggaagac
     4561 ggtcgcggcg ttggccgcaa gcccgcccgc tttcacttcg cgccgccgcg cgggctaaac
     4621 tgccggaagc ttgattctcc cgctgccata tgcaccccca ccctgcacga aacgacgaga
     4681 gggcatgatg tcggaccgcg acggccgcaa gcgcgtcatc attgagcggg tcagccccga
     4741 gatcgattgc ggccaatacg ccgcgaaaat caccgtcggc gagcgcctga cggtcgaggc
     4801 cgacgtcttc tgcgacggcc acgatcgccc ctgggccgcc ctcctcttcc gccgcgaggg
     4861 ctctccgcgt tggtccgaaa tcccgatgca gccgctcccc aacgaccgct ggcgcgcctc
     4921 gtttctcgtc accgaccagg gccgctacga atactccgtc ctcgccggcg tcgatcactt
     4981 cggcacctgg catcacgatc tgcgcaagaa aatccacgcc ggcgtcgatg tcgcggtcga
     5041 cctgctcatc ggcggccggc tgatccagcg cgccgccgaa cgcgccgaac gcgacggcgg
     5101 cggcgacggc cggcaactcg ccgaatgggc cgcgctgctc ggttccgatg cccccgtcgc
     5161 cgaaaaactc agcctgatcc aggacggtcg cctcctctcg ctgctcgcct gccatcgcga
     5221 ccgcgacctg gccttgcgct acccgcgcgt cctgcgactc atcgccgaac gcgaacgcgc
     5281 ccgcttcagc acctggtacg aactttttcc ccgctccacc tcgggcgacg aatcccgcca
     5341 cggcacgttc cgcgatgtcg agcgccgcct gcccgccatc gccgggatgg gtttcgacat
     5401 cctctacctg cccccgatcc atcccatcgg cctcactaaa cgcaagggga agaacaatgc
     5461 cctcgtcgcc gggcccgacg accccggcag cccctgggcc atcggcggac ccgagggagg
     5521 ccatacggcc gtccatcccc agctcggcac gctggccgac ttccgccgcc tcgtgcgcgc
     5581 cgctgccgat ctcggcatcg agatcgccct cgacatcgcc ctgcaatgct cgcccgatca
     5641 tccctatgtg cgcgaccacc ccgaatggtt cacccggcgc cccgacggcg ccgtccaata
     5701 cgccgagaac cccccgaaaa aatacgagga catctatccc ttcaatttcg aatgcgacga
     5761 ctggcgcgcc ctgtgggccg aaatcctcgg catcttccag cactggatcg agcacggcgt
     5821 gcgcgtcttt cgcgtcgaca acccgcacac caagcccttc cgcatgtggg actggctgat
     5881 cgaacagatc catcgcgacc accccgacgt cctcttcctc gccgaggcct tcacccgtcc
     5941 caaggtcatg tatcgcctcg ccaagggggg cttcacccag tcctatacct acttcgcctg
     6001 gcgcaacgaa aaatgggatc tgaccgagta cctgcgcgag ttgacagcga ccgaggcgcg
     6061 cgatttcttc cgccccaact tctggcccaa tacgcccgac atcctcaccg agttcctcca
     6121 gcgcaacggc cgcgccggtt tcctccagcg actcgtcctc gccgccacgc tcgccgccag
     6181 ctacggcatc tacggcccgg cgttcgagct gatggaatcg accccccgcc acgagggcag
     6241 cgaggaatac ctcaactccg agaagtatca gctccgcgcc tgggacctcg atcggcccga
     6301 cagcctcagc gacttcatcg cgcgcgtcaa ccagatccgt cgcgaaaacc cggccctcca
     6361 gtcgaaccgg aacctcgcgt tccacacgat caacaacgat cagctcatcg cctacagcaa
     6421 ggccaccgag gatcgcgcca acgtcatcct ctgcgtcgtc aatctcgacc cgcaccacac
     6481 ccagtcgggc tggatcgcgc tcgatctgaa cgctctcggc ctcgcgggcg accagccgtt
     6541 ccaggtccac gacctcctct ccaatgcccg gtttcagtgg accggcgcgc gcacgttcgt
     6601 cgagctcgac ccgcgcgtcg tgcccgcgca tatcttccgc atccgccgcc gcctccgcag
     6661 cgagcgcgat ttcgactact acctttgacg ggaggcgacg gccggcatgg cgcgcacgcg
     6721 gaaaccgccc atcgaacttg accacgatcc ccagtggtac aaggacgcta tcatctacga
     6781 gctccacgtc cgcgccttcc acgacagcga cggcgacggc tacggcgact tccgcggcct
     6841 cgcccagaag ctcgattacg tcagcgacct gggcgtcacc gcgatctggc tcctcccgtt
     6901 ctatccttcc cccctgcgcg acgacggcta cgacatcgcc aactacatgg acgtccaccc
     6961 gcgctatggc aacctgcgcg atttcaaaac cctgctggcc gaggccgaga agcgcggcat
     7021 ccgcatcatc agcgagcttg tcctcaatca cacctccgac ctgcatccct ggttccagcg
     7081 cgcccgccag gcgccccccg gcagccccca ccgcgactgg tacgtctggg aacagaaccc
     7141 cgaccgctat caggaagccc gcatcatctt ccaggattac gaacactcca actggacctg
     7201 ggaccacgtc gccaacgcct actactggca ccgcttctat tcgcatcagc ccgacctgaa
     7261 tttcgataac cccgccgtcc gccgcgagct gatcaaggtc ctcgattact ggttcgccat
     7321 gggcgtctcc ggcatgcggc tcgacgccgt cccgtacctc tacgagcagg acggcaccaa
     7381 ctgtgaaaac ctgcccagga cccacgaatt ccttaaggaa ctgcgccgcc acctcgaccg
     7441 caaattcgac ggccgcatgc tgctcgccga ggcgaaccag tggcccgagg attcgcgcga
     7501 atacttcggc gacggcgacg agtgccacat ggccttccat ttccctctca tgccgcgcat
     7561 gttcatggcc gtccagctcg aggaccgctt ccccatcatc gacatcctcg accagacccc
     7621 cgcgatcccc gacaacgccc agtgggccct cttcctgcgc aatcacgacg agctgaccct
     7681 ggagatggtc accgacgaag agcgcgatta catgtaccgc gcctatgcga gcgacctcca
     7741 ggcgcgggtc aacctcggca tccgccgccg cctcgccccc ctgctcggca acaaccgccg
     7801 caagatcgag ctgatgaacg gcctcctctt ttccctcccc ggcacgcccg tcctctatta
     7861 cggcgacgaa atcggcatgg gcgacaacat ctacctcggc gaccgcgaca gcgtccgcac
     7921 ccccatgcag tggtccgccg accgcaacgc cggcttctcc cgcgccaatc cccagcagct
     7981 ctacctgccc gtcatcaccg actacgagta ccgctacgaa acgatcaatg tcgaaaccca
     8041 gcgcaacaat ccccactccc tgctctggtg gatgaagcgc ctgatcgccc tgcgcaagaa
     8101 ctaccgcgcc ttcagccgcg gcacgatgga catcctccac cccgacaacc gcaaggtgct
     8161 ggccttcatc cgccgccacg acgaggagat cgtcctggtc gtcgccaacc tctcacgctt
     8221 cgtccaatgc ctcgagctgg atctgaaatc cttcgagggg atgacccccg tcgagctctt
     8281 cggccagaac cccttccccc gcatcggcga actgccctac ttcatcacgc tcggtcccca
     8341 cagcttctac tggttcgccc tggaacgcag cgacgccgac gcccgccggc aatccctgga
     8401 ggaacgcccc ctgccgcagc tgaccgtcgc cggcgcctgg gatcacctct tccgtcccga
     8461 gcgccgcgcc gctttcgaac ggatcctccc cgaatacctc gccctggccc gctggttcgg
     8521 cggcaaggcc cgcaaaatca agtcgacttg catcgtcgag acgatccccg tcccgattcg
     8581 cccccaccac gccctgctcg tcatgctcga agtcgaatac accgacggcg accccgaaac
     8641 ctacctgctg gctctcgcct gggccgacgg cgagcgcgct cagcaactcc agaccgcctc
     8701 ccgccacctg atcctggccc ggctcgacgt cggcgcgcgc ggcggcgagc cccgcgccgg
     8761 cgtcctctac gatgccttct gcgagcgcgc gttcaatgcc gcgctgctca cggtcctggc
     8821 ccgccgccgc aagctcgtcg gcctgcaggg ccacgtcctc ggcctcaaca ccccccagct
     8881 cgcgcggctc ctcgccggcg attcgcccga caacctcgag tcgcacgtca tccgcggcga
     8941 acaaagcaac acctcggtca tctacggcga acgcttcatc ctcaagctct tccgccgcat
     9001 cgagcgcggc atcaaccccg atatcgaatt gacccgctgc ctcaccgcgc gacgcttcgc
     9061 cccgatcgcc cctctcgccg gctggttcga gtacgtccag ggacgccgcg aaccgatcca
     9121 gctcggcctg ctccaggcct tcgtcgtcaa tgagggcgac gcctggcagt acaccctcga
     9181 cgccatgggc tggttcatgg agcaggtcct ctcgcacagc ggccgcccgc tcccccccgc
     9241 ggcgaccggc ggcctcgtcg agctctcact ccgcgaaccc cccgaggagg cccgcgaaag
     9301 catcggccac tacctcgaaa tggcgcgcct gcttggcgaa cggaccgccc agatgcatca
     9361 ggccctcgcc gccgaccccg ccgatcccgc cttcaaaccc gagtccttct cccagctcta
     9421 tcagcgctcg ctctaccagt cgatgcgcaa cctcatcggc cagaccttcc agttgctcga
     9481 ccgccaactg cgcgccctcc ccgaatcgac gcgcgatccg gcccgcgacc tgctcgcccg
     9541 ccaggacgaa ctcctcgccc ggctgcgcgc cgtcctgcag cggcgcatca ccgccaaacg
     9601 catccgcacc cacggcgatt accacctcgg ccaggtcctc tacaccggca aggatttcgt
     9661 catcctggat ttcgagggcg agcccagccg ctcgctcacc gaacgccgcc tcaaacgctc
     9721 cgccctgcgc gatgtggccg gcatgttgcg ttcctttcac tatgcggcct acaccgtctg
     9781 tcgcaatctg ttgcgcgacg gcatgattca cgaggaggat cgcccccgca tgcagcaatg
     9841 ggcccgcttc tggtaccgct gggtcgccgc ggccttcctg gccggctacc tccccatcgg
     9901 ccagggcgcc ggcttcctcc cccgcactca cgacgaactc caattgctca ttgatgccta
     9961 tatgattgat aaatgcgtct atgagctgaa atacgagctc aataaccgac ccgactgggt
    10021 ggagatcccc ctcaagggcc tgctgaatct gctggagtcc accctcgcgc cgcatgtcga
    10081 gtgatcctgg attggagcgc cccatgaccc agaccggtat gtccgtcccc cccgtcctct
    10141 acgacaacag cctgctgacc gatgacgatc tgcactggtt caacgaggga acccactatc
    10201 gcctctacca gaagctcggc tcgcacgtcg tcgagcgcga cggccgctcc ggtgtcaact
    10261 tcgccgtctg ggcccccaac gccgaggccg tcaccgtcat cggcgacttc aacggctgga
    10321 acaaggagtc ccatcccctc cgcccccgcg gccagagcgg cgtctgggaa ggcttcatcc
    10381 ccggcctggc caagggaacg cactacaaat accacgtccg ctcccggttc aatttatacc
    10441 gcgccgataa ggccgacccc tacgccgtcc accacgaaac cccgccccac accggctcga
    10501 tcgtctggga tctggactac gaatggcgcg acgccgactg gatggcccac cgccacgcgc
    10561 gcaacagcct caacagcccc atcgcgatct acgaggtgca tctgggctcc tggcgacgca
    10621 atcccgcgga aaatcaccgc ccgctcaatt accgcgaact cgcccccctc ctcgccgatt
    10681 atgtcgccga ctccggcttc acccacgtcg agttcctccc catcatggaa caccccttct
    10741 acggctcctg gggctaccag accaccggct acttcgcccc caccagccgc tacggcaccc
    10801 cccaggactt catgttcctc atcgactacc tgcaccagca cggcatcggc gtcatcctcg
    10861 actgggtccc ctcgcacttc cccaccgacg agcacggcct cggctatttc gacggcaccc
    10921 atctctacga acacgccgac ccccgccaag gctttcatca ggactgggcc agcctcatct
    10981 tcaactacgg ccgcaacgag gtgcgcaact tcctcatgag cagcgccctc tactggctcg
    11041 accgctatca cgtggatggc ctccgcgtcg acgccgtcgc ctcgatgctc tacctcgact
    11101 actcacgcaa ggccggcgaa tgggtcccca acgccttcgg cggacgcgaa aatctcgagg
    11161 cgatcgactt cctccgccgc ttcaacaccg aagtcttcca gcatttcccc gacgtccaga
    11221 ccttcgccga ggaatcaacc gcctggccca tggtctcccg ccccacctac gtcggcggcc
    11281 tcggcttcgg cctcaaatgg gacatgggct ggatgcacga taccctctcc tacttccaga
    11341 aggatccgat ccatcgcaaa taccaccacg gccagatcac cttccgcatg ctctatgcct
    11401 tcaacgaaaa cttcatcctc cccctctccc acgatgaagt ggtccatggc aagcgctccc
    11461 tcctcgacaa gatgcccggc gatcgctggc gcaaattcgc caacctgcgc ctgctgttca
    11521 gctatatgta cgcccagccc gccaaaaaac tcctcttcat gggcggcgaa ttcggccagt
    11581 ggcgcgagtg gtcccacgac gaaagcctcg actggcacct cttcctcgaa cccgaccaca
    11641 agggcctgca caacctcgtc cgccagctca accggatcta ccgcgaagag cccgccctcc
    11701 atcacttcga tctcagcagc gatggcttcg agtggatcga ctgcaacaac accgaggaca
    11761 gcacggtcat cctcctgcgc cgcgggccgg ggggcgatcg cgacgtcatc atcgccgcct
    11821 tcaactttac ccccgtcgtc cgctacaact accgcgtcgg cgtcgtccac aagggcctct
    11881 ggcgcgaaat cctcaactcc gatgccctgg aattcggcgg cagcggcgtc ggcaacatgg
    11941 gcgccgtcca cagcgatccc atcccgttcc atggccgccc ccaatcgatc aacatcaccc
    12001 tgcccccgct gggcgcggtc ttcttccgtc atgagggctg actatgccga ctttctctcc
    12061 ggccgcaggc tgggcgcaac gccgggtgca actggccgcg cgcggttttc ggtctgggcg
    12121 ccggcggccc gccgggtcga actccacctc cacggcgcgc gcgaggaatt cctcgatctc
    12181 tcctccggcg gcgacggcct cttctcgcgc gaggtccccg atgtcccccc cggcgcgctc
    12241 tacacctatc gcctcgatgg ccggcgcgag ttccacggta actttcgttt tgagtccttc
    12301 aatcgccttt ttcatgcggg tgaccgcgtc gttggcggcc tcgaccttgg aggcgtcgac
    12361 gcgactatag a
//
LOCUS       DAOPCO010000097        12322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5088_length_12322_cov_5.547648, whole genome shotgun sequence.
ACCESSION   DAOPCO010000097 DAOPCO010000000
VERSION     DAOPCO010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_12322_cov_5.547648"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLS90_08370"
     CDS             <1..288
                     /locus_tag="PLS90_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HPK02458.1"
                     /translation="DADVILGENKDLFLIPDILCNAGGVVVSYFEWVQDIQMFFWSEE
                     EVVRRLREIMISRFEEVHRYARDNNVSMRTAALSLGVGRIAQEKTQRGLYP"
     gene            complement(351..2156)
                     /locus_tag="PLS90_08375"
     CDS             complement(351..2156)
                     /locus_tag="PLS90_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPK02459.1"
                     /translation="MQDYTLLVNLSLMFAIGLGLGLFTHRLGLSPIVGYLLTGIALGP
                     STPGFVADAHMATEFAEIGVILLMFGVGLHFHIGDLIKVRSVALPGAFAQVVVATFLG
                     VLVSLMFGRTITVGVVLGLAVSVASTVVLIRVLMDNNVLETGHGHLAVGWLLVEDLFT
                     VLALVILPGMAAVLSGRGDAAGADPWLSLAGAMGRIVLLVTAVMVLGKRAVPWLLRQV
                     ARSRSRELFTLTVLALALAIATGSAVGFGVSMALGAFLAGMVVGQSEVSHQAAADALP
                     MRDAFAVLFFVSVGLLFDPKVILENPGFLIGLLIVIMIAKPLTAFIICWLFGRSVRTG
                     LTVGLALAQIGEFSFILGSAAVQLGMVSAASQSLLVTCAVITIALNPPLFRLIGPVEN
                     WIRRRPKLWRLLNRRSELRGEELNASTRARLMTQGDVPRTSALVVGYGPVGQTVAEIL
                     RRFHIQPVIIDMNLNTVESLSAAGVMAIYGDAARREVLESAGIARASYLIVTPPEPAI
                     RTSIIMAARSVHAGLKIIVRTRYLSERQWQESLGVAGFACEEVEVAIGLAAELLEDFG
                     VGDSRLQSEVREMRHALEAGLLADAHRNGDHAPQT"
     gene            complement(2325..2780)
                     /locus_tag="PLS90_08380"
     CDS             complement(2325..2780)
                     /locus_tag="PLS90_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02460.1"
                     /translation="MMSSPNVQTVEILLVEDNPGDVRLTLEAFKDITIANRLHVVPDG
                     EEAIKFLRHQHGYADSPRPHMILLDLNLPLMNGIEVLREIKKDNELRSIPVVVLTTSR
                     NEEDILKAYNLNANCYITKPVDINQFMQVVRTIDNFWFSIAALPGRTLT"
     gene            complement(2939..4012)
                     /gene="hisC"
                     /locus_tag="PLS90_08385"
     CDS             complement(2939..4012)
                     /gene="hisC"
                     /locus_tag="PLS90_08385"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPK02461.1"
                     /translation="MSPTPPLPEPNAFLAEIKPYEPGEQPQAPGFIKLNTNEFPYPAA
                     PEVIEAIKREAADTVRVYPDPTCRALREALAAKHGLHPEQIIVGNGSDEILRLLFHAY
                     VGAGRNLGIVQPTYTLFEVLARQFGARTLVFPLDASEKLSAGFLAAPWDAVFLPVPNP
                     PIGGVFPERDLRKLAERGKLLVLDGAYIDFAQDHDPANLLAEFPNVVLTRSFSKSHGL
                     AGMRLGYAMAHREIIANLNKLRDSYNVDRVTQAAGLAALAADRYYAKRCAEIVESRRK
                     LAADLTKRGFKVPASQANFVFARHARAPEIYDALKQRKILVRHFNHSGLQGGVRITIG
                     TPEENKALLYALDAIMPEGNFRR"
     gene            4275..5450
                     /locus_tag="PLS90_08390"
     CDS             4275..5450
                     /locus_tag="PLS90_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02462.1"
                     /translation="MMNEALLQIVRNEPSWCLSTRSTRLAVTRVGGHVAPVTFFADSA
                     APIEPYYIAPWWDEGTDPELPSLLAVLRGDFFCAPFGNNEKPRDGKHYPPHGESANAR
                     WQLEEVRHGAAGARLRMSQAQTITGGRIEKTLLLVEGHQAVYSRHVLTGMEGPMPIGH
                     HPNHYIPDDAGQAYLSFAPYTHAHTYVEPVERPEHRGYSILQPDTAIEDLRRCPRRDG
                     TTTDLTRYPARRGYEDIVILAGCKGEPFGWSALALPSRGYVWFSLKDPRVLVSTLLWF
                     SNGGRHVAPWSGRNHNCIGIEEITGFFHAGIHACAEKNFLSEMGIATHVMLKRDEALA
                     VNFIQGVARINPDFKGVSAIEAKDEETIRIVDEQGQAVEAKVEWKFARDGETKDFRD"
     gene            5688..5957
                     /locus_tag="PLS90_08395"
     CDS             5688..5957
                     /locus_tag="PLS90_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02463.1"
                     /translation="MAKKAAKKAPAKKSAAKKKTATKRPVKKATKTAKKSVKKAAKKT
                     AKKAVKKTARKTAAKKATKKAAKKAAPKKAAKKTTKKAAKKTAKK"
     gene            complement(6562..7914)
                     /gene="miaB"
                     /locus_tag="PLS90_08400"
     CDS             complement(6562..7914)
                     /gene="miaB"
                     /locus_tag="PLS90_08400"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPK02464.1"
                     /translation="MTRTYHITTFGCQMNAYDSDVMEGIMTAKGWTRADREEEADVIL
                     YNTCVVRDHAEQRALARLDQLARLKRERPDRIIGVTGCMAQKEAEALHERLPHVDLVL
                     GTRAIARLSPLLDRVIATGTPQACTEILDDGYPADVVPVRQRPLKALVNIIFGCNKNC
                     SYCIVPRTRGREVSRPWRDIVEEARRLRAHQYREITLVGQNVNSYRQPGEGTRSFGNL
                     LRRVGEAAGGADPDAAWIRYITSHPRDCNAAHIRAVAETPNVCENFHLPVQAGANRVL
                     RRMRRSYTRERYLELIDEVRAAVPDATLTTDIIVGFPGETPDEFDQTLDLVRRVRFDS
                     AYMYMYSTRPGTPAAEEFKDHIPLAEKKARLAALIALQEQISLELNRAAIGRRFAVLI
                     EDVAPRTPGDLLARTRGDKMVILPGPREWVGRFAEVEVHDANGHTLFARPVAVARAAA
                     "
     gene            8041..8187
                     /locus_tag="PLS90_08405"
     CDS             8041..8187
                     /locus_tag="PLS90_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02465.1"
                     /translation="MNAKQFCDWQTAGGTDDVMRLVDCRERADILWCAIGGIAANPWA
                     KERS"
     gene            8196..8498
                     /locus_tag="PLS90_08410"
     CDS             8196..8498
                     /locus_tag="PLS90_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02466.1"
                     /translation="MDFVVATDAVECATRALEDAGFRADHHPWSIHFKGRSQVVLQLS
                     TEAFCSEFASRAVPAGVHGILMRVASLHDMAAIPFASAAATGDPVGQTPLTVLRPR"
     gene            8495..9712
                     /locus_tag="PLS90_08415"
     CDS             8495..9712
                     /locus_tag="PLS90_08415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPK02467.1"
                     /translation="MTSPLHPRATLRPDRDKPVRQRHPWVFSGALARIDDAAEDGGLV
                     DLFSAGGEFLARGYLNRRSQIMIRLLTWDAAEAIDDAFWRGRIARACAGRVIPPDGAG
                     RLVYSESDGLPGLILDRYGDWLVVQALTLGIETRKQQLARVARELTGARGVFERSDVD
                     VREKEGLAPATGLLAGEEPPAELTFVESAFDDRPLRQIVDLRRGHKTGAYLDQAINRR
                     RVGRWCAALGPGAQCLNAFAYTGAFALHLLAAGAAGVLNIDSSAEALATARRNLELNG
                     FAPNDDDFHCANVFEALRGLRDRARRFDLIVLDPPRLAQSAAHVDRAARAYKDLSMLA
                     LKLLRPGGLLATFSCSGQISAELFQKIVFSAALDAGRDAQIVERFTQAPDHPVLLSFP
                     EASYLKGLLLRAG"
     gene            complement(9729..10292)
                     /locus_tag="PLS90_08420"
     CDS             complement(9729..10292)
                     /locus_tag="PLS90_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02468.1"
                     /translation="MRAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVSRMKADLR
                     TLATAVEAYAVDNNAYPPHRADDGGEIGYPARYVPLTTPVAYIASIPARDVFHQEDVT
                     GQGGSLEWVSWTNIDNFPATHALDPARDTHRWMLRSRGPDGLSEANDVRNAFLVFGLA
                     LGPSMLYDPTNGTVSRGDIVRTAILSP"
     gene            complement(10378..11466)
                     /locus_tag="PLS90_08425"
     CDS             complement(10378..11466)
                     /locus_tag="PLS90_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPK02469.1"
                     /translation="MSPTSPGSGPSSPADPARFRTYPRLSRLWKQSTRRLGLDATTRA
                     ELAAWRRRLRRHLRRLTGYDRLAPAPLAPRLTEEVACDGYRRQRVEIQVEPGLFMPLY
                     VLIPAGSAGPWPPVMALHGHGSGGKYAVAGRRDLPDLDETIRIHNYDYGVQLVREGFI
                     VFCPDARGFGERQEARVEGGLLVSSCLAINQMALPLGRTVTGMWAWDVHRLVDYILTR
                     GDCRAAPLGCMGLSGGGLQTLWAAALDDRIGYSVVSGYFYGYREALFDVYVHCSCNYV
                     PGLFEAIDMGDLGALIAPRPLCIETGARDPLNGAGGLKNVRSQVRIARQAYRMLGASR
                     ALTHAVFDGPHRWDGTEAIPWMKRAAGL"
     gene            11696..>12322
                     /locus_tag="PLS90_08430"
     CDS             11696..>12322
                     /locus_tag="PLS90_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02470.1"
                     /translation="MTETLTQPRKTRYAIVGTGGRCEMFRDALNTDFSAHGEIVGFCD
                     VNRGRLLRYQEETRKATGRVVPIFDLAANPDGFDEMVRRTQPDVVIVTTIDRFHHQYI
                     VRAMELGCDAISEKPMTMEADKCQKIIDAQARTGRRCRVTFNYRYSPPRVQVKDLLMS
                     GIIGDIVSVDFHWMLDTHHGADYFRRWHRNKENSGGLMVHKATHHFDLV"
BASE COUNT         2188 a         3791 c         4399 g         1944 t
ORIGIN      
        1 gacgccgacg tgatcctcgg cgaaaacaag gatctgttcc tcattccgga catcctgtgc
       61 aatgccgggg gcgtggtcgt cagctacttt gagtgggtgc aggatatcca gatgttcttc
      121 tggtcggagg aggaagtggt gcgccggctg cgcgagatca tgatcagccg gttcgaggag
      181 gtccaccgct atgcgcgcga caacaacgtc tcgatgcgga cggcggcgct gagcctgggc
      241 gtcgggcgca tcgcccagga gaagacgcag cgcggattgt atccgtaagc gagtcgtttt
      301 cgagagccga cgacgagaac gagggacgac agggagaagt gcgggggcgg tcaagtctgc
      361 ggtgcgtggt ccccgttacg atgggcgtcg gcgaggaggc cggcttccag agcgtggcgc
      421 atctcgcgaa cctcggactg gagacggctg tcgccgacgc cgaagtcctc gagcagttcg
      481 gcggccaggc caatcgcgac ctcgacctcc tcgcaggcaa accccgcgac gcccaggctc
      541 tcctgccact ggcgttcgct caaatagcgc gtgcggacga tgatcttcag cccggcatgg
      601 accgaacggg ccgccataat gatcgaagtg cggatcgccg gctcgggcgg ggtgacgatc
      661 aggtaacttg cccgggcaat gccggcgctt tcgaggacct cgcggcgcgc ggcgtcgccg
      721 tagattgcca tcacgccggc ggcgctgagc gactcgaccg tattcaggtt catgtcgatg
      781 atgaccggtt ggatgtggaa ccggcggagg atttcggcga cggtctggcc gaccgggccg
      841 tagccgacga cgagcgcaga ggtccgcggg acgtcgcctt gcgtcatcaa gcgggcgcgg
      901 gtgctggcgt tgagctcctc gccgcggagc tcggagcgcc gattgagaag gcgccacaac
      961 ttcgggcggc ggcgaatcca gttttcgacc ggcccgatca ggcggaaaag cggcggattg
     1021 agcgcgatcg tgataacggc gcaggtgacc agcaggctct ggctggcggc cgagaccatc
     1081 cccagctgga ccgcggcgct gcccaggatg aacgagaact cgccgatctg ggccaacgcg
     1141 aggccgacgg tcagtccggt ccggaccgag cggccgaaga gccagcagat gatgaacgcg
     1201 gtcagcggtt tggcgatcat gatgacgatc agcagaccga tcagaaagcc gggattttcg
     1261 aggatcacct tgggatcgaa gagcaggccg accgagacga aaaagaggac ggcgaaggcg
     1321 tcgcgcatcg gcaacgcatc ggcggcggcc tgatggctca cctcggattg cccgacgacc
     1381 atgccggcga ggaaggcgcc cagggccatc gagacgccga agccgacggc cgaaccggtg
     1441 gcgatggcga gcgccagcgc cagcaccgtc agcgtgaaca gttcgcgcga ccgtgagcgc
     1501 gcgacctggc gcagcagcca ggggacggcc cgtttgccga ggaccatcac cgccgtgacg
     1561 agcaacacga tgcggcccat ggcgccggcg agcgagagcc agggatcggc cccggctgcg
     1621 tcgccccggc cgctcagcac cgcggccatg ccgggcagga tcaccagcgc cagcacggtg
     1681 aagagatcct cgacgagcag ccagccgacg gccagatggc catggccggt ctccagcacg
     1741 ttgttgtcca tcaggacgcg gatgaggacg accgtgctgg cgaccgagac ggcgaggccg
     1801 aggacgacgc cgacagtgat cgtgcgtccg aacatgagcg agacaaggac gccgaggaac
     1861 gtggcgacga cgacctgggc gaatgcgccg gggagggcga ccgagcgcac cttgatcaga
     1921 tcgccgatgt ggaagtgcag gccgacgccg aacatcagca ggatcacgcc gatctcggcg
     1981 aattccgtcg ccatgtgggc gtcggcgacg aagccggggg tcgaagggcc cagcgcgatg
     2041 cccgtcagca gatagccgac gatcggcgac aggccgagcc ggtgcgtgaa cagcccaagc
     2101 cccagcccga tcgcgaacat gagcgagaga ttgacgagca gggtatagtc ttgcatccga
     2161 agagccaccg ccggatgggg aatacaagcc gtgggggaga gattttaact ggcggttgtt
     2221 tggtggtcaa aaaaaagtga gggaccggat gccgcaacga attcggcgca ttgcggtgca
     2281 acgctctgcc accgcactcc cgtaagccgg atgtgtctct gcgatcaggt cagggttcgg
     2341 ccgggcaagg cggcgatgga gaaccagaag ttgtcgatcg tccgcacgac ctgcatgaac
     2401 tgattgatat cgaccggctt ggtgatgtag cagttggcgt tcaagttata ggccttgagg
     2461 atgtcctctt cgttgcgcga ggtcgtcagg acgacgaccg gaatcgatcg cagctcgtta
     2521 tccttcttga tttcgcgcaa gacctcgata ccgttcatca gcggcaggtt caggtcgagc
     2581 agaatcatgt gcggacgcgg gctgtcggcg tagccgtgct gatgccggag aaacttgatg
     2641 gcttcctcgc cgtccgggac gacatgcagc cggttcgcga tcgtgatgtc cttgaaggct
     2701 tccagggtga ggcggacatc gccggggttg tcttcaacca gcagaatttc aacggtctgg
     2761 acgttgggcg agctcatcat tgcgcttctt tcccggcccg ggaggacgac ccgagatcgg
     2821 ataatctccc gggcagtctg ttccgacacg aaaggatcgg gtgggccagt cgatcccaaa
     2881 cccgaacata acggagggga aatgcaggaa gcgtgccggg tgagccggtt gtcggcgctc
     2941 agcgccggaa gttgccttca ggcatgatgg cgtccagggc gtagagcagg gccttgttct
     3001 cttccggggt gccgatcgtg atgcggacgc cgccctgcag gccgctgtgg ttgaaatggc
     3061 ggaccaggat tttgcgctgc ttgagggcat cgtagatttc gggggcgcgg gcgtggcgcg
     3121 caaagacgaa attcgcctgg ctcgccggca ccttgaatcc gcgcttggtc agatcggcgg
     3181 ccagcttccg gcgggattcg acgatttcgg cgcagcgctt cgcatagtag cgatcggccg
     3241 cgagcgcggc caggccggcc gcctgcgtga cgcggtcgac gttgtaggag tcgcgcagct
     3301 tgttcaggtt cgcgatgatc tccctgtgcg ccatggcgta gcccagccgc atcccggcca
     3361 ggccgtgcga cttggagaag gaacgcgtca agacgacatt ggggaattcg gccagcaggt
     3421 tggcgggatc gtggtcctgg gcgaagtcga tataggcgcc gtcgaggacg agcaacttgc
     3481 cgcgctcggc cagcttgcgc aggtcgcgtt cggggaagac gccgccgatg ggcgggttgg
     3541 ggacggggag gaagacggcg tcccacggcg cggcgaggaa tccggcgctg agcttctcgc
     3601 tggcatccag cgggaagacg agcgtgcgcg cgccgaactg ccgcgccagc acctcgaaga
     3661 gcgtataggt gggctgaacg atgccgaggt tgcggcccgc gccgacatag gcgtgaaaca
     3721 gcaggcggag gatctcatcg gaaccgttgc cgacgatgat ctgctcggga tgaaggccgt
     3781 gtttggcggc gagcgcctcg cgcagcgcgc ggcacgtggg atcggggtag acgcggacgg
     3841 tgtcggcggc ttcgcgcttg atcgcctcga tgacttccgg cgcggcgggg taggggaatt
     3901 cgttcgtgtt gagcttgatg aagcccggcg cctggggctg ctcgcccggc tcatacggct
     3961 tgatctccgc caggaacgcg ttgggctcgg gcagcggcgg cgtggggctc atcgtcggac
     4021 tcgattctgg atgttgggat cgcgcggatc gccgggagat cgttgcatcg ggcgcgaacc
     4081 gccgcatccg ttgtaattcg cgcgggggcg aatggcaagc agagcgaaaa ggtgggatac
     4141 gcgggaagga gcggtgacca ggacgaggac gagggacgat tctggagtga ggggcgtgaa
     4201 gggggttgcg agggcgattg gcgagggata ccgtttgtgc gtattgagat ctcgcccgaa
     4261 ggaaggggga cccgatgatg aacgaagcct tgctgcagat cgtccgcaat gaaccgtcgt
     4321 ggtgcctgtc gaccaggtcg acgcggctgg cggtgacccg cgtcggcggg catgttgcgc
     4381 cggtaacgtt tttcgcggac agcgctgcgc cgatcgagcc gtactacatc gcgccgtggt
     4441 gggacgaggg aaccgatccc gagctgccgt cgctgctggc ggtgctgcgc ggcgactttt
     4501 tctgcgcgcc gtttggcaac aacgagaagc cgcgcgacgg caagcactat ccgccgcatg
     4561 gggaatcggc caatgcccgg tggcaactgg aggaggtccg ccacggcgcc gcgggggcgc
     4621 gcctgcggat gtcgcaggcg cagacgatca cgggcggccg gatcgagaaa acgctgctgc
     4681 tggtcgaggg gcaccaggcg gtctactcgc gccacgtgct gaccggcatg gaagggccga
     4741 tgccgatcgg ccaccatccc aaccactaca tcccggacga cgcggggcag gcgtatcttt
     4801 cgttcgcgcc gtacacgcat gcacatacct acgtggagcc ggtcgaacgg ccggagcatc
     4861 gaggctattc gatcctgcag ccggacacgg cgatcgagga tctgcgccgc tgcccgcggc
     4921 gcgacgggac gacgaccgat ctgacgcgct accccgcgcg gcgcggctac gaggacatcg
     4981 tgattctggc cggatgcaag ggggaaccgt tcggctggag cgcgctggcg ctcccgtcgc
     5041 gcggctatgt gtggttcagt ctgaaggatc cacgcgtgct cgtttccacg ttgctgtggt
     5101 tctccaacgg cggccggcac gttgcgccgt ggagcggccg caaccacaac tgcatcggca
     5161 tcgaggagat caccggcttc ttccatgcgg ggatccacgc gtgcgcggaa aaaaattttt
     5221 tgagcgagat ggggatcgcg acgcacgtga tgttgaagcg cgacgaagcg ctggcggtga
     5281 acttcattca gggcgtggcg cgaatcaacc ccgattttaa aggggtttcg gcgattgaag
     5341 cgaaggatga agagacgatc cgcatcgtgg acgagcaagg ccaggcggtt gaagcgaagg
     5401 tcgaatggaa gtttgcgcgc gatggagaga caaaagattt tcgcgattga agtttttcat
     5461 cttgacacga aacacgactc ttgaaaaaaa catggttgaa gcgtgcatca tggtggagca
     5521 cgcgccgcgt tgtttgcgaa ccgcagacct acttgggacg atgacaggac ctctcggcag
     5581 gcatctttcg cgcaagcgaa cctgttcctg aaatcgaacc aaccgcaaac gacgcccggg
     5641 gtgtggttca acactacctc ctgactgcag gaaaggagac tttctttatg gcaaagaaag
     5701 cagcgaagaa agcaccggcc aagaagtcgg cggcgaagaa gaagaccgcg acgaagcggc
     5761 cggtaaagaa ggcgacgaag accgcgaaga agtcggtcaa gaaggcggcc aagaagacgg
     5821 cgaagaaggc cgtgaagaag acggcgcgca agaccgcggc gaagaaggcg accaagaagg
     5881 cggccaagaa ggctgctccg aagaaggccg ccaagaagac gacgaagaag gccgccaaga
     5941 agaccgccaa gaagtagccc ggcggattga accccggagc cgacggcccg ccgagaccgt
     6001 ccgctccgcg ggttctggat cggcatcgac ccgcaacgag acccacagcg gtcgcctcgc
     6061 cacacccctt caagcagtcg catggctttt cccatgccct tcttgcagac cacaccccac
     6121 ccggtggggt gaccgctggg cgaaacgaac gggccgaccg accgatcgag cggcggcgca
     6181 gagccgctcc cggtggagca accgatcatg aaaacccgga tgacgcttcg gcgtcaggcc
     6241 gggtttttct ttgtaagcca ttcaacgacc agtggccgcg tcgtctctcg tcctcgtcct
     6301 cgtggttgaa cgtcgtcggt ccgatccgac ggaccggacg gatttcgatg gcgaatacga
     6361 gttcttcatg tagctgcggt tcgcagcgaa gcgacaagag gcaggttttc gagagccgag
     6421 gacgagggac gagagccgag agccgagagc cgaaactttg agcgggccgt tgggggcgat
     6481 cacgcggacg agtgacgagg gaggcagagg tcggggcggc ggcgacggcg cggcgaggca
     6541 agccgggttg ggctggctcg ctcaggcggc ggcccgcgcg acggcgaccg ggcgggcgaa
     6601 gagcgtgtgg ccgttggcat cgtggacttc gacttcggcg aaccggccga cccactcgcg
     6661 cgggccgggc aggataacca tcttgtcgcc gcgcgtgcgc gccagcagat cgcccggcgt
     6721 gcgcggggcg acgtcctcga tcagcacggc gaagcgccgg ccgatcgccg cgcggttgag
     6781 ctccaggctg atctgctcct ggagcgcgat cagggcggcg aggcgcgcct tcttctccgc
     6841 cagcgggatg tggtccttga actcctcggc ggcgggcgtg ccgggccgcg tggaatacat
     6901 atacatatag gccgagtcga agcggacgcg gcggacgagg tcgagcgtct gatcgaattc
     6961 atccggcgtc tcgccgggga agccgacgat gatgtcggtc gtcagcgtcg cgtcggggac
     7021 ggcggcgcgc acctcgtcga tcaactccag gtaacgctcg cgcgtgtagg agcggcgcat
     7081 gcggcgcagg acgcggttgg cgccggcctg gaccggcaga tgaaagttct cgcagacgtt
     7141 gggggtctcg gcgacggcgc ggatgtgcgc cgcgttgcag tcgcgcgggt gcgaggtgat
     7201 gtagcgaatc caggcggcat cgggatcggc gccgccggcg gcctcgccga cgcggcgcag
     7261 caggttgccg aaactgcgcg ttccctcgcc cggctggcgg tagctgttga cgttctggcc
     7321 gacgagcgtg atctcgcgat actgatgcgc gcggagccgg cgcgcctcct cgacgatgtc
     7381 gcgccacggc cggctgacct cgcgcccgcg cgtgcgcggc acgatgcagt aggagcagtt
     7441 cttgttgcag ccgaagatga tgttgaccag cgccttgagc gggcgctggc ggaccggcac
     7501 cacgtcggcc gggtagccgt cgtcgaggat ttcggtgcag gcctgcgggg tccccgtcgc
     7561 gatcacgcgg tcgagcagcg ggctcagccg cgcgatggcg cgcgtgccga ggaccagatc
     7621 gacatggggg agccgttcgt ggagcgcctc ggcttccttc tgcgccatgc agccggtcac
     7681 gccgatgatg cggtcgggac gctcgcgctt gagccgggcc agttggtcga gccgggcgag
     7741 ggcgcgctgc tcggcgtgat cgcgtacgac gcaggtgttg tagaggatga cgtcggcctc
     7801 ttcctcgcgg tcggcgcgcg tccagccctt ggccgtcatg atcccttcca tgacgtccga
     7861 gtcataggcg ttcatctggc agccgaaggt ggtgatgtga taggtccgag tcatcgcggg
     7921 caacatttta cggtcggcgg cggggcggaa caagggagaa accggaacgt cggcgcccgc
     7981 gccgcttccc aatcgacacc ggggcccgtc aatcctgcta ttcgcgaaca ggtgcgggtg
     8041 atgaacgcca aacaattctg cgactggcag accgccggcg gaacggacga tgtcatgcgg
     8101 ctcgtggact gccgcgagcg ggccgacatc ctgtggtgtg cgatcggcgg aattgcggcc
     8161 aacccttggg cgaaggaacg gtcttaacgc aagacgtgga ctttgtcgtc gcgaccgatg
     8221 cggtcgagtg cgcgacccgg gcgctcgagg acgcggggtt ccgcgccgat caccatccat
     8281 ggtcgatcca tttcaaaggt cgctcccagg tcgtccttca actctcgacg gaggcctttt
     8341 gtagcgaatt cgcgtcgcgc gcggtgccgg ccggcgtgca tggcatcctg atgcgggtcg
     8401 cctcgctgca cgacatggcg gcgattccct ttgcatcagc ggccgcaacc ggcgatcctg
     8461 tgggccaaac tccgctgacg gtcctccggc cccgatgacg tctccgctcc atccccgcgc
     8521 cacactccgc cccgaccgcg acaagccggt ccgccagcgc catccgtggg tgttctcggg
     8581 cgcgctggcg cggatcgacg acgcggccga ggatggcggc ctggtcgatc tgttcagcgc
     8641 cggcggcgag tttctcgcgc gcggttacct caatcgccgc tcgcagatca tgatccggct
     8701 gctgacgtgg gacgcggccg aggccatcga cgatgcgttc tggcgcggcc ggatcgcgcg
     8761 cgcatgcgcc ggccgcgtga tcccgcccga tggggccggt cgcctcgtct actccgaatc
     8821 cgacgggctg cctggtctga tcctcgaccg ttacggcgac tggctggttg tgcaggcgct
     8881 gacgctcggc atcgagacgc gcaagcagca gcttgcccgg gtcgcccggg aactgaccgg
     8941 cgcgcgcggc gtgttcgagc ggagcgacgt cgatgttcgg gagaaggagg gtctggcgcc
     9001 cgccacggga ctcttggcgg gggaggagcc gcccgctgag ttgacgtttg ttgaatcggc
     9061 gttcgacgac cgtccgctgc gccagatcgt cgacttgcgg cgcgggcaca agaccggcgc
     9121 gtatctggac caggcgatca accgccgacg cgtcggccgc tggtgcgccg cgctcggccc
     9181 gggggcgcaa tgtctcaatg ccttcgccta caccggcgcg ttcgcgctgc atctgctcgc
     9241 ggcgggcgcc gccggtgtgc tgaacatcga cagctcggcc gaagcgctcg cgacggcgcg
     9301 gcgcaatctt gaactgaacg gcttcgcgcc gaacgacgac gatttccact gcgccaacgt
     9361 ttttgaggcg ctgcgcggcc tgcgcgatcg cgcgcggcga ttcgatctga tcgtgctcga
     9421 cccgccccgg ctggcgcagt cggccgcgca cgtcgaccgc gccgcgcggg cctacaagga
     9481 cctgagcatg ctcgcgttga agctgctgcg ccccggcggg ttgctggcga cgttttcgtg
     9541 ctccggccag atctcagccg aattgtttca gaagatcgtc ttcagcgcgg cgctcgatgc
     9601 cggccgcgac gcgcagatag tcgagcggtt cacgcaggcg cccgatcacc ccgtcctgct
     9661 cagcttcccc gaggcgtcgt atttaaaagg gctgctgctg cgggccgggt gatgttccca
     9721 ccctgccgtc agggcgacag gatcgcggtg cggacgatat cgccgcgcga gacggtgcca
     9781 ttggtcgggt cgtaaagcat cgacggtccc agcgccaggc cgaagaccag aaacgcgttg
     9841 cgcacgtcgt tggcttcgct caggccgtcg gggccgcgac tgcgcagcat ccagcggtgc
     9901 gtatcgcgcg ccggatcgag cgcgtgcgtc gccggaaaat tatcgatgtt cgtccaggag
     9961 acccattcca gcgagccccc ctggccggtc acgtcctcct ggtggaagac gtcgcgcgcc
    10021 gggatcgacg cgatgtaggc caccggggtc gtcagcggaa cgtagcgcgc ggggtagccg
    10081 atctcgccgc cgtcgtcggc gcgatgcggc ggataggcgt tgttgtcgac ggcgtaggcc
    10141 tcgaccgccg tcgccagcgt gcggaggtcg gccttcatcc ggctgacctt ggagcgggtc
    10201 tgcgcctcca ggaagttggg cacggcgatc gccgccagaa tcgcgatgat cgcgacgacg
    10261 atcagcagtt cgatcagggt gaaggcgcgc atggggctgc tttcggttgg ctgtggggaa
    10321 atcccggggt cctcacttta acagccgccg aatgaaagat cacgtccggt gaggggctca
    10381 cagtccggcg gcgcgcttca tccaggggat cgcctcagtc ccgtcccagc ggtgcgggcc
    10441 gtcgaagacc gcgtgcgtca acgcccgcga tgccccgagc atgcggtagg cctgacgcgc
    10501 gatgcggacc tgcgaccgga cgttcttcaa cccgccggcc ccgttgagcg gatcgcgtgc
    10561 gccggtctcg atgcacagtg gccggggcgc gatcagcgcg ccgaggtcgc ccatatcgat
    10621 cgcttcgaac agccccggca cgtagttgca cgagcagtgg acataaacgt cgaacagcgc
    10681 ctcgcggtag ccgtagaaat agccgctcac gacgctgtag ccgatccggt cgtccagcgc
    10741 ggcggcccac agcgtctgca ggccgccgcc cgagagcccc atgcagccca gcggagcggc
    10801 gcggcagtcc ccccgggtca ggatgtaatc caccagtcga tggacgtccc aggcccacat
    10861 cccggttacg gtccggccca gcggcagcgc catctggttg atcgcgaggc aggaggagac
    10921 cagcaggccg ccctcgacgc gggcctcctg gcgctcgccg aagccgcgcg cgtcggggca
    10981 gaagacgatg aacccctcgc gtaccaactg cacgccgtaa tcatagttgt ggatgcggat
    11041 tgtctcgtcc aggtcgggca ggtcgcggcg gccggccacc gcatacttgc cgccgctgcc
    11101 gtggccgtgc agcgccatga ccggcggcca gggcccggcc gacccggccg gaatgagcac
    11161 gtagagcggc atgaagaggc ccggctcgac ctgaatttcc acgcgctggc ggcggtagcc
    11221 gtcgcaggcg acctcctcgg tgaggcgggg cgccaacggc gcgggcgcga gccggtcata
    11281 ccccgtgagg cggcgcaggt gccggcgcag gcggcggcgc cacgccgcca gctccgcgcg
    11341 cgtcgtcgcg tcgagtccca gccggcgggt cgactgcttc cacaggcggc tcaggcgggg
    11401 gtaggtgcgg aagcgggcgg ggtcggccgg cgagctcgga ccggagcctg gcgaagtggg
    11461 ggacatgaag ggatctccta taagtagaac gggattgagg cgggagggaa aacggggtag
    11521 cctgacgcat ccacgcaacg gccgccgtgg gcgaggtaaa ggatgtaagg cgagggggga
    11581 ggcggctgcg ggcgcggaga cgtaaacgct tacgtgaccg ccccacgcct gcttccgccc
    11641 cgaccggggc aatggccgcc gggcgcgccg ccacctcacc ccaggatcca aatccatgac
    11701 cgaaacgcta acacaaccgc gcaagacgcg ctatgccatc gtcggcacgg gcgggcgctg
    11761 cgagatgttc cgcgacgccc tcaacaccga tttctccgcc cacggcgaaa tcgtcggctt
    11821 ctgcgacgtc aatcgcgggc ggctcctgcg ctatcaggag gagacgcgca aggcgaccgg
    11881 ccgggtcgtg ccgatcttcg acctggccgc aaaccccgac ggcttcgacg aaatggtccg
    11941 gcgaacccag cccgacgtgg tgatcgtcac gacgatcgac cgcttccatc atcaatatat
    12001 cgtgcgcgcg atggaactgg gctgcgacgc catcagcgag aagccgatga cgatggaagc
    12061 cgacaagtgc cagaagatca tcgacgcgca ggcgcgcacg ggccggcgct gccgggtcac
    12121 gttcaactat cgctactcgc cgccgcgcgt gcaggtcaag gatctgctca tgtcggggat
    12181 catcggcgac atcgtgtcgg tcgatttcca ctggatgctt gacacgcatc atggggccga
    12241 ctacttccgc cgctggcacc gcaacaagga gaactccggc ggcctgatgg tgcacaaggc
    12301 gacgcaccat ttcgacctgg tc
//
LOCUS       DAOPCO010000098        12299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5095_length_12299_cov_4.829141, whole genome shotgun sequence.
ACCESSION   DAOPCO010000098 DAOPCO010000000
VERSION     DAOPCO010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5095_length_12299_cov_4.829141"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..416
                     /locus_tag="PLS90_08435"
     CDS             147..416
                     /locus_tag="PLS90_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02471.1"
                     /translation="MRLTAVRNLTCCLLLAGAGLLALPGCGDQEPVDAMDSMTTPAND
                     LPASDVSAPAPEAQVHDESEPGHVHDASEADHVHTDEMTTETAQQ"
     gene            795..1157
                     /locus_tag="PLS90_08440"
     CDS             795..1157
                     /locus_tag="PLS90_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02472.1"
                     /translation="MIDDEANVRHLLELLLRDAGFDVRAFGSPAEALTGLGARPPDLI
                     ISDQDLADEMNGVELIGKLREAAGQPVLPAMLLTGNVNSWDEGDEPEMAGIIVLRKPF
                     GIVELQAQVERLLGVNAC"
     gene            1164..1322
                     /locus_tag="PLS90_08445"
     CDS             1164..1322
                     /locus_tag="PLS90_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02473.1"
                     /translation="MGGSAIPRAEHLEKEKEQRLRRTCVEAEPLILVVLGVAIGSIAL
                     RCCCRCRR"
     gene            1563..2078
                     /locus_tag="PLS90_08450"
     CDS             1563..2078
                     /locus_tag="PLS90_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02474.1"
                     /translation="MRKAARRGFTLVELIIVIVILGILGTIAIPQLISSTKDAEEATL
                     AADLTMLRNSISLYYHQHNSVYPGVVTSDGAGTATDATNNVAAFISQLREYSDKSGRT
                     SAVLDRANFPFGPYLNNGLPENPINGLATVKMLTDSDAIASGEIDGTTGWLYNSTTGE
                     IRSNTTGYLEY"
     gene            complement(2174..2983)
                     /locus_tag="PLS90_08455"
     CDS             complement(2174..2983)
                     /locus_tag="PLS90_08455"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPK02475.1"
                     /translation="MVDSGSNPFDLSGRVALVTGASRGLGQYMARALARAGADLVITS
                     RNRDSLTDFQREIEALGRRALPLPLDVTDEGSIRAMARDALAQYGRIDILVNNAGRNR
                     RKPALDVTWEDWDFVVNANLRGTFFVAQAVAPSMIERGYGRIINIGSVTCVFGYAGLA
                     PYCASRGGTRQLTMSLADEWGPYGVTVNCLAPGWFKTAQTAVLYNDPEWVEYLVDRIP
                     VKRPGLPTDLDGAVVFLASEASRYVTGQTLLVDGGISTGATRAMPVKQSEK"
     gene            complement(3310..5271)
                     /locus_tag="PLS90_08460"
     CDS             complement(3310..5271)
                     /locus_tag="PLS90_08460"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPK02476.1"
                     /translation="MGTNVAHKLIREHLLEGEMTPSREIGLRIDQTLTQDATGTLVML
                     ELEAMGLDRAKTEVSAQYVDHNLLQTDHKNPDDHIFLQSACERFGLWFSRPGNGVSHP
                     VHMHRLGRPGKTLLGADSHTCAAGAIGMLAIGAGGLDVAMAIAGYPFYVKMPKIWGVR
                     LTGKLPPWVSAKDVILELLRRHGVAGGVGRIIEYYGPGLDQLSAWDRHTICNMGAELG
                     ATTSVFPSDGETRRFLHSEGRPADWIELTADADAHYDLHDEIDLEQLEPLIAKPSSPG
                     NVVPVREIAGLPIYQAYVGSSANPGFRDLAIPAAMVRGRRVHPHVSFDLNPASRQILE
                     NLIQEGWLGPLVHAGGRLHQTGCNGCIGMGQAPASGRNSLRTVPRNFPGRSGTREDSV
                     FLCGPETATASALRGVITDPRDLDMEYPQIRELDRQIINATMLDPPLPPDEARRRELI
                     KGPNIASLALLEPIADTLELVVLLKVGDNLSTDEILPAGTRVLPYRSNIPKISEFCFD
                     MVDPTYHERALGVRDAGGHAVVGGANYGQGSSREHAALAPRYLGLALVLARSFARIHW
                     QNLVNFGILPVSFADPADYDRIEQGDKLRVENVRERVQQGRVLTVRITGKRGEREIPV
                     RHDLSGRQVAILLAGGQINRVREEAGKKK"
     gene            5531..6295
                     /locus_tag="PLS90_08465"
     CDS             5531..6295
                     /locus_tag="PLS90_08465"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HPK02477.1"
                     /translation="MLQIVRKLESFRIGATDGEIGNLHDVLFDDEFWTVRYLVVDTGN
                     WLPGRKVVIPPDKVSLRSHKDNVIPVDLTREKIKASPPLEVERPVSRQHQIDLYAYYG
                     WPVYWGGGMGPAAPYPYVPLMGAMTGSEPAALATEEEEPRREDDPHLRSAREVTGYHV
                     EAIDGGIGHVEDFIVDDETWQVRYLIVDTKNWLPGRKVLIAPLWLHRFDWIAQKAYLD
                     LDKEQIRNSPPYEEHVDLTRSREEEIYDYYGKPRYW"
     gene            complement(6369..6950)
                     /locus_tag="PLS90_08470"
     CDS             complement(6369..6950)
                     /locus_tag="PLS90_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02478.1"
                     /translation="MLRGCARLLLLTLGLAALGCRPDGPASQSPGTRPNEAPAGETTP
                     QPVLSEAAAPAAAAPAAAETEPADAATTAPHRVRLVWQSEMEENAFGYTVYRRETEAG
                     DEIALNPENPLHAAGTTHIPQEYTFYDLNVQPGVTYYYRIRATDLDGSYAWPFPPAEA
                     RPKPLTPAEAAEIEANGTMYRRPLEGAELPGNK"
     gene            complement(7291..7560)
                     /locus_tag="PLS90_08475"
     CDS             complement(7291..7560)
                     /locus_tag="PLS90_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK02479.1"
                     /translation="MTKTQLVNHLAEGVGLTKRQAADALNVLAETAYDEAKRGFTLPG
                     IGKLVVVKRKARKGRNPATGETIKIPAKTVLKFRVAKACKDRVLS"
     gene            complement(7904..10774)
                     /locus_tag="PLS90_08480"
     CDS             complement(7904..10774)
                     /locus_tag="PLS90_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HPK02480.1"
                     /translation="MLRKEPPSAVRQALSRLGIAWDQVLLCTDTDIDRMGRYVRKWLV
                     ITAQRLLVISEEEPAEVVREIDLNQVEEYRCQSVIGSGLLQVKVDGMYIDMLRYSNRM
                     GDKFHKVARKLERFRLGEPIVLHPEDDIDPRRCPSCNLMLSFPGDVCPRCVSKGAVLA
                     RMWVLMKPYRGAALTMMALLMVGIALDLVAPQLTRFLVDRVLPGSPEQLGEIRANLQL
                     YQHLLGVLLTVVGVLALTNVSRMGINMINGRLGNKIGTAITFDMRGRLVSHLQQLSVG
                     YYDRQQVGSLVGRVAYDTESLHGFVNQLTSGFLFQLLMVIGVGTMMFILNAKLALFTL
                     IPAPLVIAGSIFFWRYIYPRYYRFWDASSKQAGMLSGVLSGIRVVKAFSQEQREIDRF
                     NRVSGRLRDNRLHVDNATALFNPCMAIIFQMGGWIVWYVGGRDVLGGTMTLGSLIAFF
                     AYQAMFYGPLTTLTQFTNWLTQFATQAHRIFEILDTPIEITEARDAIPLERINGHILF
                     DNVTFGYTRNMPVIQDVTLEIQPGEMIGVVGRSGSGKTTLINLLSRFYDVELGAVKID
                     GIDVRRIRKDDLRSRIGVVLQEPFLFRGSIWDNIAYGRPDATPTEVFEAAKAGNCHDF
                     IMRQAHGYDTWIGERGAGLSGGERQRIGISRVLLTDPKILILDEATSNVDAESEAAIQ
                     AALAEVVRNRTTIAIAHRLSTLRSADRIVVVDRGRIAEMGTHQELLERDGIYARLVKI
                     QGHMTTQPTIEQIAQREEDEARDGQRQADEDNLPIGPNHVGPLPHPRSHHVRWLTPDR
                     AHIHSGSYNTLHLTVEGEGIYCGLFALRCLPVHYPEEYISLRMTGARGREIEVGIIRR
                     IADWPETTQQLVREALLKRYLVHTITAIESIQQFSGYLNFRVQSDLGPLEFMLRWQAD
                     RAHDYGPNGRMLLDTEENRYLIPDLNRLTEHERKLFQRYIYW"
     gene            complement(11096..>12299)
                     /locus_tag="PLS90_08485"
     CDS             complement(11096..>12299)
                     /locus_tag="PLS90_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPK02481.1"
                     /translation="SRLHTAVKIALQYSAPTVHVKDASRVVGVVQALLDPQRRDEYAA
                     RVRAEYDALREHHGRGREEKTLIAYADARANAFRSDWDTLDIPTPEFRGLRVLDDYPI
                     DELVRYIDWTPFFHAWEMKGIFPRILDHPERGAEARKLYDDARRLLERIIRERRLRAR
                     GVVFFHPANAIGDDVEVYTDERRGGLLATFHFLRQQGERPDGKPNYSLADFIAPRESG
                     RLDYLGGFAVTAGHGVAELVAEFQADHDDYQAIMVKALADRLAEAFAERLHELVRAWW
                     GYGQGENLSQEALIAERYRGIRPAAGYPACPDHTEKRILFDLLGAERNAGIELTENFA
                     MTPGAAVSGLYFAHPAALYFDVGRLGLDQVENYAERKGMELHEVERWLGPNLGYEPAA
                     PARAAATV"
BASE COUNT         2136 a         3666 c         4334 g         2163 t
ORIGIN      
        1 gcgccggcgg cggcggcgcg ccgtgagctt cggatgggac aggtccgatg ttgcggaaca
       61 gatggatttg actgaaaatc ggcgagaatc ggacgggatg cgcccgccga tcaacataaa
      121 gcccccaaag aaagggcccc ctaccgatgc gactcaccgc tgttcggaat ctgacctgct
      181 gcctgctcct ggccggcgcg ggtctgctgg cgctgccggg atgtggagac caggagccgg
      241 ttgacgcgat ggattcgatg acgacgccgg ccaacgacct gcccgcttcg gatgtcagcg
      301 cccccgcccc ggaggcgcag gtccatgatg agagcgaacc gggtcatgtc catgatgcga
      361 gcgaagcgga tcatgtccac accgacgaga tgacgacgga gaccgcgcag cagtaagccg
      421 cgcgctcctt ctcgtttgca acgcgggggc cggcgtggcg gcgggcaggg cgaccgggtt
      481 gaaaccggtt gccggtcgcc gcgccggtcc ccgtttttgc tgggagagcg cggcccggtc
      541 gtggaggggt gtttcgccgg gtccctggtg cacggcagcg agttcctctg attaacgttg
      601 ttgtttttgt atcgtttggc tgcaacctac actttcccgc cccgcctgcc gaaaagaaac
      661 gggatacggc ggggcttgcc ctttaagccc gcccttgcct ggcatcggac tctgccgcga
      721 aaccgcatca atccctcgat cggcgactga ccggcgaggc agaaccacag gaattcgtga
      781 tgaagtgtgt attcgtgatc gacgacgagg cgaacgtccg ccatctgctg gagttgcttc
      841 tgcgcgacgc gggcttcgac gtgcgggcgt tcggctcgcc ggcggaggcg ttgacgggcc
      901 tcggcgcgcg cccgccggac ctgatcatca gcgatcaaga cctggccgac gaaatgaacg
      961 gcgtggagct gatcgggaag ctgcgcgagg cggccggaca gccggtattg ccggcgatgc
     1021 tgttgaccgg caacgtgaac tcgtgggatg aaggtgacga gccggagatg gcgggcatca
     1081 tcgtcctcag gaaaccgttc ggcatcgttg agttgcaggc gcaggtggaa cggttgctgg
     1141 gcgtgaacgc gtgctgaggg gtgatgggag gaagcgcgat tccgagggcg gagcacctgg
     1201 aaaaggagaa ggagcagcgg ctgcgccgga cctgcgtcga ggccgaaccg ctgatcctcg
     1261 tggtgctggg cgtcgcgatc gggtccattg cgctgcgctg ctgctgccgc tgtcgaagat
     1321 gacgccggtg gcccgcagcg gccactgatc agaagcaaga aatccgaatt tcgaaatccg
     1381 aatcagaatt cgcggcagtt tacgcgacgg gtagcaccgg gatcggctcg cgggcccagc
     1441 tccgggataa cggatatatt taacgttatt aacgatttgc cgggtgagaa cggtcaagtt
     1501 tcttccagcc tggtcgatac actcattgga cgtacgaaca cgtttgcaga aagggtactg
     1561 cgatgaggaa ggctgcccgc aggggcttca cgctggttga gctgatcatc gtcatcgtga
     1621 tccttggcat actggggacg attgccattc cgcaacttat cagttccacc aaggacgccg
     1681 aggaagccac gctggccgcc gacctgacca tgttgcgcaa ctccatcagc ctgtattatc
     1741 accagcacaa ctccgtctac cccggcgtgg tgacgtccga tggggcgggc acagccaccg
     1801 acgcgaccaa taacgtggcg gccttcatca gccagcttcg ggagtactcg gacaagagcg
     1861 gcaggacgag cgcggtgctc gatcgagcga acttcccgtt tgggccgtac ttgaacaacg
     1921 ggttgcccga aaatccgatc aacggcctgg cgacagtcaa gatgctgacc gattccgacg
     1981 cgatcgcttc cggcgagatt gacggcacga ccggctggct ctacaacagc acgaccggcg
     2041 agatccgctc caacacgacc ggttatctgg aatattagcc caagcctgct ggaagatacg
     2101 caacgggttg caagccagcg ccaatgcgcc aatggcgccg tcgccgggcc agccggccga
     2161 ctcgcaattt cccttatttt tccgattgtt tcaccggcat ggcgcgcgtg gcgccggttg
     2221 aaatcccgcc atcgacgagc agcgtctggc cggtcacgta gcggctcgcc tccgacgcga
     2281 ggaagacaac cgccccgtcc agatcggtcg gcaggcccgg ccgcttgacg gggatccgat
     2341 cgacgagata ctcaacccac tccgggtcgt tgtagagcac ggcggtctgg gccgtcttga
     2401 accagccggg cgcgaggcag ttgaccgtga cgccgtatgg cccccattcg tcggcgaggc
     2461 tcatcgtgag ctggcgcgtg ccgccgcggc tggcgcagta gggggcgagg ccggcatagc
     2521 cgaacacgca agtaaccgaa ccgatgttga tgatgcggcc atagccgcgc tcgatcatgc
     2581 tgggggcgac cgcctgggcg acgaaaaacg tgccgcgcag gttggcgttg acgacgaaat
     2641 cccagtcctc ccaggtgacg tcgagcgcgg gtttgcggcg gttgcggccg gcgttgttga
     2701 cgaggatgtc gatgcggccg tattgcgcga gcgcgtcgcg ggccatcgcg cggatgctgc
     2761 cctcgtcggt gacgtcgagg gggagcggca gggcgcgccg gccgagcgct tcgatttccc
     2821 gctggaaatc ggtcaaggaa tcgcggttgc ggctggtgat gacgaggtcg gcgccggcgc
     2881 gggcgagcgc gcgcgccatg tactggccca ggccgcggct ggcgccggtg acgagcgcga
     2941 cgcggcccga aagatcaaat ggattgctgc cggaatcaac catttagccc ctccgcggaa
     3001 attcgaagct tgaatctcga aatccgaagc aaattcaaag atcaaagatc aaagatcaag
     3061 taaccaaacg ccgctatccc ttccgggaaa atacgaagct cgaattgcaa aatttgacac
     3121 aattaaaaat atccaatgct atatcacgaa attccctgat tccagagggc gaacccccgg
     3181 gtgcgcgtgc acgccgggcc atccagccgc tcgatattct ggcgaatata atcctgattg
     3241 cattccgcaa tattcaaatt ttgaatttgt ttcggacttc gagcttcgta tttcgaattt
     3301 ctctttcatt cattttttct ttcctgcttc ctcccgcacg cggttgatct ggccgcccgc
     3361 cagcaggatc gccacctggc gtccggacag gtcatgacgc acggggatct cgcgctctcc
     3421 gcgcttgccc gtgatccgga ccgtgagtac gcggccctgc tgcacccgtt cgcgaacatt
     3481 ctccacgcgc agcttgtcgc cctgctcgat tcggtcgtag tcggccgggt cggcaaaact
     3541 caccggcagg atgccgaaat taacgaggtt ttgccaatgg attcgggcaa agctccgggc
     3601 aagcacgagc gccaggccga gatagcgggg cgcgagcgcg gcatgttcgc ggctggagcc
     3661 ctggccgtag ttggcgccgc cgacgacggc gtggcccccc gcgtcgcgca cgcccagcgc
     3721 gcgctcgtga taggtcgggt caaccatgtc gaagcagaat tcgctgattt tcgggatatt
     3781 gctgcgatag ggaaggacgc gggtgccggc cggcaggatt tcgtcggtcg acaggttgtc
     3841 gccgaccttg agcagcacca ccagttccag cgtgtcggcg atcggttcga gcagcgccag
     3901 cgaagcgatg ttcggaccct tgatcagctc gcgccggcgc gcttcgtccg gcggcagcgg
     3961 cggatcgagc atcgtcgcat tgatgatctg ccggtccagc tcgcggatct gcgggtattc
     4021 catgtccagg tcgcgcggat cggtgatgac gccgcgcagg gccgaggcgg tggccgtctc
     4081 cgggccgcag aggaagaccg aatcctcgcg ggtgcccgag cggcccggga aattgcgcgg
     4141 cacagtgcgc aggctgttgc gtcccgacgc cggcgcctgg cccatcccga tgcagccgtt
     4201 gcagccggtc tggtgcaggc gtccgccggc gtggaccagc ggaccgagcc aaccctcctg
     4261 gatcagattc tcaagaatct ggcgcgaggc gggattgagg tcgaacgaga catggggatg
     4321 cacgcgccgg ccgcggacca tcgccgccgg gatggccagg tcgcgaaacc cgggattggc
     4381 cgacgaaccg acatacgcct gatagatcgg caggccggcg atctcgcgca cgggaaccac
     4441 gttgcccggg ctggagggct tggcgatgag cggttccagt tgctcgaggt cgatctcgtc
     4501 gtgcagatcg tagtgcgcat cggcgtcggc ggtcagttcg atccagtcgg ccgggcggcc
     4561 ctcgctgtgg aggaagcggc gcgtttcgcc atccgagggg aagacgctgg tggtcgcgcc
     4621 gagctcggcg cccatgttgc agatcgtgtg gcgatcccac gcgctgagct gatcgaggcc
     4681 ggggccgtag tattcgatga tcctgccgac gccgccggcg acgccgtggc gccgcagcag
     4741 ctccaggatg acgtccttcg ccgagaccca cggcgggagt ttgccggtca gccgcacgcc
     4801 ccagatcttt ggcatcttga cgtaaaacgg atagccggcg atcgccatgg cgacatcgag
     4861 tccgcccgcg ccgatggcga gcatcccgat cgccccggcg gcgcacgtgt ggctgtcggc
     4921 ccccagcagc gtcttgccgg ggcggcccag ccggtgcatg tggaccggat gcgagacgcc
     4981 gttgcccggc cggctgaacc agaggccgaa gcgttcgcag gcgctctgca ggaaaatgtg
     5041 gtcgtcggga ttcttatggt ccgtctggag caggttgtgg tcgacatact gggcggagac
     5101 ctcggtcttc gcccggtcca gccccatcgc ctccagttcg agcatcacca gcgtgccggt
     5161 cgcgtcctgc gtcaacgtct gatcgatgcg caggccgatt tcgcggctcg gcgtcatctc
     5221 gccttccagc agatgttcgc ggatcagctt gtgggctacg ttggttccca tgatggtgcc
     5281 tccggttcat gccggtgagt cgtcagtcaa ctcgaaccgt cgaggcgcgc ttccggtcgc
     5341 ggatcgcgcg ccctggcctc ctcggtcacg gttgatgatc ggtttgggaa gaatcccttc
     5401 gggaaaaata ggagcgaaaa gcatgccgcg cccgccatcg ctcctgcact gctgaagggc
     5461 acggttgttg ttagcaagat cattctgctt gatccgggga aggaccgcga tagaaaggtg
     5521 ggacctggcg atgttacaga tcgtacgcaa actggagagc ttccgcatcg gcgcgaccga
     5581 cggcgaaatc ggcaatctgc acgacgtttt gttcgacgac gaattctgga cggtccgtta
     5641 cctcgtcgtc gacacgggca actggctgcc ggggagaaag gtggtgattc cgcccgacaa
     5701 ggtgagtctg cgctcgcaca aggacaacgt gatcccggtc gacctgacgc gggagaagat
     5761 caaggccagt ccgccgctgg aggtggagcg tcccgtctcg cgccagcacc agatcgatct
     5821 ctacgcgtat tacggctggc cggtttactg gggcggcggg atggggccgg cggcgcccta
     5881 cccttacgtg ccgctgatgg gcgccatgac cggcagcgaa ccggcggcgc tggcgaccga
     5941 ggaggaggaa ccccggcgcg aagacgaccc gcatctgcgc agcgcccgcg aggtgaccgg
     6001 ctaccatgtc gaggcgatcg acggcgggat cggccacgtc gaggatttca tcgtcgacga
     6061 cgaaacctgg caggtgcgct atctgatcgt cgataccaag aactggctgc ccggccgcaa
     6121 ggtgctgatc gcgccgctgt ggctgcaccg gttcgactgg atcgcgcaga aggcctacct
     6181 cgatctcgac aaggagcaga tccgcaacag cccgccgtac gaggagcatg tcgacctgac
     6241 gcgctcgcgc gaggaggaaa tttacgacta ctacggcaag ccgcgctatt ggtgagccgt
     6301 gcccatcggt ctcgtatcgg tatctggatc gatttcggta tcgtaatcgg gcagatgaac
     6361 ctgctcgctt acttgttgcc cggcagctcg gcgccctcaa gcgggcggcg gtacatcgtc
     6421 ccgttcgcct cgatctcggc ggcttcggcg ggcgtgagcg gtttgggtcg tgcctcggcg
     6481 gggggaaacg gccaggcata cgaaccatcc agatcggtgg cgcggatgcg gtagtaatag
     6541 gtgacgcccg gctgcacgtt caagtcgtag aaggtgtatt cctgcgggat gtgcgtggtg
     6601 ccggcggcgt ggagcggatt ttcgggattc agggcgattt cgtcgcccgc ctcggtctcg
     6661 cgacggtaaa ccgtgtagcc gaacgcgttc tcctccatct cgctctgcca gacgagccgg
     6721 acgcgatggg gggccgtcgt ggcggcgtcc gccggttcgg tctcggcggc ggcgggcgcg
     6781 gcggcggcgg gtgcggcggc ttcggatagc accggttgcg gagtcgtttc gccagcgggg
     6841 gcttcgttgg ggcgggtacc tggggattga ctggccggac cgtcggggcg acagcccagg
     6901 gcggccagcc ccaacgtcag gagcagcagg cgagcacacc cgcgcaacat ggcaacctcc
     6961 gcggaggaat agcggcaagc aacaccgaag ccaactgccg ccgtctggcc gaagattata
     7021 gtcccccggc ccagcggttg ctccctcgat ttcgttttcc gcgagcagac gtgaagggtc
     7081 gggcaatgcg agggtttcga gagccgggga cgagagccga gagccgggag ccgaattctg
     7141 gagaaggccg ttgggggcga atacgacgac gagggacgag gggcggatgc ttcgggaagg
     7201 gaatgtgagg cgcaccggaa taaagaaaag gcggcgagga atggggtcct cgccgcccgt
     7261 ggaaatgatt ccgctttcat ccgcggggaa tcaggacaac acgcgatcct tgcaggcctt
     7321 ggccacgcgg aatttgagca ccgtcttggc cgggattttg atcgtttcac cggtggcggg
     7381 attgcgcccc ttgcgggcct tgcgcttgac gacgaccagc ttgccgatcc cgggcaaggt
     7441 gaagccgcgt ttggcctcgt cgtaggccgt ctcggccagg acgttgagcg catcggcggc
     7501 ctgacgcttg gtcaagccga cgccttccgc cagatgattg accagttgag tcttggtcat
     7561 cgctttcttc gtcgcagtcg ccataagccg cacccttcct gtcgaaatag tccgaccatc
     7621 catcagtagg attgaaactc accggccatt atgatgaggc gcgcctgact gtcaatgggc
     7681 aaaacacgcg gcgaggcgct tttccgcgcg gccgacgggg cccgtgaacc accgaatcga
     7741 ccggtcgaga cccctgccgg accgatccgc caacccgttc cgccagggac ggcagaccac
     7801 ccgcccgagc cgaatcctgg gcgggccgtt gggggcgatt acgaggacga gggagtagag
     7861 acgaaggagg agagacgagg gaggagagac gcactttggg gggttaccag taaatgtagc
     7921 gctggaagag tttgcgttcg tgctccgtca gcctgttcag gtcggggatc aggtagcgat
     7981 tctcctcggt gtcgagcagc atgcggccgt tggggccgta atcgtgggcg cggtcggcct
     8041 gccagcgcag catgaattcg agcgggccca ggtcgctctg aacgcggaag ttgaggtagc
     8101 cgctgaactg ttgaatgctc tcgatggcgg tgatggtgtg caccaggtag cgcttgagga
     8161 gggcctcgcg caccagttgc tgagtcgtct cgggccagtc ggcgatgcgg cggatgatgc
     8221 cgacctcgat ctcgcgtccg cgcgcgccgg tcatccgcag cgagatgtat tcctcggggt
     8281 aatgcacggg caggcaacgc agggcgaaca ggccacaata gatcccctcg ccttcgacgg
     8341 tcaggtgcag ggtgttgtag ctgcccgagt gaatgtgggc ccgatcgggc gtcagccagc
     8401 gaacgtgatg gctgcggggg tgggggaggg ggccgacatg gttcgggccg atgggcaggt
     8461 tgtcctcgtc ggcctggcgc tggccgtcgc gggcctcgtc ttcctcgcgc tgggcgatct
     8521 gttcgatcgt gggctgggtg gtcatgtgtc cctggatctt gaccaggcgc gcgtagatcc
     8581 cgtcgcgctc gagcagttcc tggtgggtgc ccatttcggc gatgcgtccg cggtcgacga
     8641 cgacgatgcg gtcggcgctg cgcagcgtgg agagccggtg ggcgatggcg atggtggtgc
     8701 ggttgcggac gacttcggcg agcgcggcct ggatggcggc ttcgctttcg gcgtcgacgt
     8761 tgctggtggc ttcgtccagg atcaggatct tggggtcggt cagcagcacg cggctgatgc
     8821 cgatgcgctg gcgctcgccg ccggagaggc cggcgccccg ctcgccgatc caggtgtcgt
     8881 agccgtgcgc ctggcgcatg atgaagtcgt ggcagttgcc cgccttggcg gcctcgaaga
     8941 cctcggtggg ggtggcgtcg ggtcggccgt aggcgatgtt gtcccagatg ctgccgcgga
     9001 agaggaaggg ctcctgcagc acgacgccga tgcggctgcg caggtcgtcc ttgcggatcc
     9061 ggcggacgtc gatgccgtcg attttgacgg cgcccagttc gacatcatag aagcgcgaga
     9121 gcaggttgat cagcgtcgtc ttgcccgagc cggagcggcc gacgacgccg atcatctcgc
     9181 ccggctggat ctccagcgtg acatcctgga tcaccggcat gttgcgcgtg tagccgaagg
     9241 tgacgttatc gaacaggatg tggccgttga tccgctcgag ggggatcgcg tcgcgcgcct
     9301 cggtgatttc gatcggtgtg tcgaggatct cgaagatccg gtgcgcctgc gtggcgaact
     9361 gcgtcagcca gttggtgaac tgggtcaggg tcgtcagcgg cccgtagaac atcgcctgat
     9421 aggcgaagaa ggcgatcagg ctgccgagcg tcattgtgcc gcccagcacg tcgcggccgc
     9481 cgacatacca gacgatccag ccccccatct ggaagatgat cgccatgcac gggttgaaca
     9541 gggcagtggc gttgtcgacg tggaggcggt tgtcgcgcag gcggccgctg acgcggttga
     9601 agcggtcgat ctcgcgctgc tcctggctga aagccttgac gacgcgaatg ccggacagca
     9661 cgcccgacag catgccggcc tgcttgctgg aggcgtccca gaagcggtag tagcgcggat
     9721 agatgtagcg ccagaagaag atcgagccgg cgatgaccag cggggcgggg atgagcgtga
     9781 agagcgccag cttggcgttc aggatgaaca tcatcgtgcc gacgccgatc accatcagca
     9841 gctggaacag gaagccgctg gtgagctggt tgacgaagcc gtggagcgat tcggtgtcgt
     9901 aggcgacgcg gccgacgagc gagccgacct gctgccggtc gtaatagccg accgaaagct
     9961 gctggaggtg gctgaccagc cggccgcgca tgtcgaacgt gatcgcggtg ccgatcttgt
    10021 tgcccagccg gccgttgatc atgttgatcc ccatccggct gacgttggtc agcgcgagga
    10081 cgccgacgac tgtcagcagg acgccgagca ggtgctgata gagctggaga ttggcgcgga
    10141 tctcgccgag ctgttcgggg ctgccgggca ggacgcgatc gacgaggaag cgggtcaact
    10201 gcggggcgac cagatcgagc gcgataccga ccatcagcag cgccatcatc gtcagcgcgg
    10261 cgccgcggta gggcttcatc aggacccaca tgcgggccag gacggcgccc ttgctgacgc
    10321 agcgcgggca gacgtcgccg ggaaacgaga gcatcaggtt gcaggagggg cagcggcgcg
    10381 gatcgatgtc gtcctcgggg tgcaggacga tcggctcgcc caggcggaag cgctccagct
    10441 tgcgggcgac cttgtggaac ttgtcgccca tgcggttgct gtagcgcagc atgtcgatgt
    10501 acatgccatc gactttgacc tggagcaggc cggagccgat gacgctctgg cagcggtact
    10561 cctcgacctg gttcagatcg atctcgcgga cgacttcggc cggctcctcc tcgctgatga
    10621 cgagcagccg ctgggcggta atgacgagcc acttgcggac gtagcggccc atgcggtcga
    10681 tgtcggtatc ggtgcagagc agcacctgat cccaggcgat gccgaggcgg gacagggctt
    10741 gcctgaccgc cgacggcggt tctttcctca gcatgagcgg cgccttgtag cgtgtggagg
    10801 gcttgggtcg gaagccggcc gacggggcac acgggggggt cgcatggatc ggggtaaccc
    10861 gctcaagtgc tgcccgtact gtagcgtgtg caagcgggag ggcaagcaaa aagagggggg
    10921 cgttttggcg tgagaccacc cgcggcgcgc cggggcggaa gaacgcgagc cgggagacgg
    10981 atttggggcc tgcctggggg ggcgattacg aggacgcgga cgagggacga aagacgaagg
    11041 gattgggaaa tccggcggat cggtccgacc cgacggattt ccgcacggcg ccgggctaga
    11101 ccgtggcggc ggcgcgggcg ggggcggccg gctcgtagcc cagattgggg ccaagccagc
    11161 gttccacctc gtgcaattcc atccccttgc gttcggcgta attctccacc tggtccaggc
    11221 cgaggcggcc gacgtcgaag tagagcgcgg cgggatgcgc gaagtagagc ccgctgacgg
    11281 cggcgcccgg cgtcatcgca aagttttcgg tcagctcgat gccggcgttg cgttcggcgc
    11341 cgagcaggtc gaacaggatc cgcttctcgg tgtgatcggg gcaggcgggg tagccggcgg
    11401 cggggcggat gccgcggtag cgttcggcga tcagggcttc ctggctcagg ttttcgccct
    11461 ggccatagcc ccaccaggcg cgcacgagtt cgtgcagccg ctcggcgaaa gcctcggcca
    11521 gtcggtcggc cagtgccttg accatgatcg cctggtaatc atcgtgatcg gcctggaact
    11581 cggcgaccaa ttcggcgacg ccatggcccg ccgtgacggc aaacccgccg agatagtcga
    11641 gccggccgct ttcgcgcggc gcgatgaaat cggccaggct gtagttcggt ttgccgtcgg
    11701 ggcgctcccc ctgctggcgc aggaagtgga acgtggcaag caggccgccg cggcgttcgt
    11761 cggtgtagac ttcgacatca tcgccgatgg cgttggccgg atggaagaaa acgacgccgc
    11821 gggcgcgcag acggcgctcg cgaatgatgc gctcgagcag ccggcgcgcg tcgtcgtaga
    11881 gcttgcgggc ttcggcgccc cgctcgggat gatccagaat cctggggaag atccccttca
    11941 tctcccaggc gtggaagaac ggagtccagt cgatgtaacg gacgagttcg tcgatggggt
    12001 aatcatccag cacgcgcagg ccgcggaatt cgggggtcgg gatgtcgagc gtgtcccaat
    12061 cggaacggaa ggcgttggcc cgggcgtcgg cgtaggcgat cagggttttc tcttcgcggc
    12121 cgcggccatg gtgctcgcgc agcgcgtcgt actcggcccg cacgcgcgcg gcgtattcgt
    12181 cgcggcgctg gggatcgagc agcgcctgga cgacgccgac gacgcgcgag gcgtccttga
    12241 cgtgaacggt cggggcgctg tattgcagcg cgatcttgac ggcggtgtgc agccggcta
//
LOCUS       DAOPCO010000099        12254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5122_length_12254_cov_5.014018, whole genome shotgun sequence.
ACCESSION   DAOPCO010000099 DAOPCO010000000
VERSION     DAOPCO010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12254
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5122_length_12254_cov_5.014018"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1113
                     /locus_tag="PLS90_08490"
     CDS             223..1113
                     /locus_tag="PLS90_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02482.1"
                     /translation="MMPSPPPATTLPQPLDRPPAGPRWWLRLRRQPSFWITSAALLAL
                     VAVLWLGPALSPYSEREQDLTAIYAGPSAQRWFGADGLGRDLMTRVFHGGRISLAVGV
                     VGALVSLILGVAVGGVAGYAGGRTEQLIMRLIDFLYGVPLLLVVIALMVVTGPGLVNI
                     FIALGLVYWLGMARVVRARVAELRSRDYVTAARALGAGPARILLRHVLPNAAGVIVVT
                     ATFMIPQAIFAESFLSFLGLGVTLPHASWGTLAADGLDAMRSHPHVLAFPAVAICLTM
                     FLFQALGESLRAALDPRGED"
     gene            complement(1133..2854)
                     /locus_tag="PLS90_08495"
     CDS             complement(1133..2854)
                     /locus_tag="PLS90_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02483.1"
                     /translation="MKEQQPTRTFTPGELSGGWQLTLRPRELVPGLKPEYIIDQFPAA
                     IGRHPTNDIELPFDAVSRYHARIEIYGGKPRIIDLRSSNGTYVNGKRVQVAPIVDQDG
                     ISFGSLEFSLTIQEFDSDQRVITRSDQTSAVHFVQQDDMVQTVIHADLPDDTSVPHLI
                     LEEDITDEETLRKAKIRLICLYRLQEVLRSTADEEKLMRNVLRLLFEVLPVDRGVMLM
                     RDKDDPAVFRPCAVKVRDGLASESIGISRTILQRGLRERIAILTRNAPEDSRFQGAES
                     IVANSMRSVMCVPLISARHVFGFCHLDTVEPVRMFTQEDLTFLATVCAEMATHLHNLR
                     MVQDKIQTERMAAIGQTITGMAHNIKNILLLSQGGVEMMDKQLERKQYDTVEETWGVV
                     RRGLERIHGLVADMLDYSRARTIEKRPIDLGEFLTELCATFADEMRKRNIDCRLELAP
                     DLPRILVDVDGLEKAVVNLIVNALEALPEDKGTVTLRTLRNDEGHAQIEVEDDAGGIP
                     AEVLPRIFVPFFTTKGSKGSGLGLAMTRKFIEDMGGRIEAHSSEGVGTRFTITLYTEP
                     EGPRLKE"
     gene            complement(2942..3547)
                     /locus_tag="PLS90_08500"
     CDS             complement(2942..3547)
                     /locus_tag="PLS90_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02484.1"
                     /translation="MDGLITLIIVFVVFWSFMKKVQQVMQEMRERYSKLDEGDAPPTG
                     REMEVQRELQRRIEELERQRKADVSAQRRAAGQSSLDAPAAARPGPVVVAAPPRRLKP
                     VDAGEPRPMAPAAPAARRAISVMPVMEEPPRAEAPVPVEPARQPRPAAPRPAPRRPAG
                     RPGRAVAQLRFSPSMMRRVVVLNEIFQPPVALRDNPPWGRR"
     gene            complement(3564..4064)
                     /locus_tag="PLS90_08505"
     CDS             complement(3564..4064)
                     /locus_tag="PLS90_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPK02485.1"
                     /translation="MKLFLLRHGEAVDAAEVGGDDRARTLTKRGRRQGKIAGKALRRL
                     DWRPAVVVTSPYPRAAETARRAAKALKGDVELVVDAALEPGASPEAMLECVGRHGDEG
                     DLLLVGHNPDLERLIACLVGGGAAGIELEKGAVAALDLGEEPPAPGRATLLALAPPAL
                     LEALVD"
     gene            complement(4693..6141)
                     /locus_tag="PLS90_08510"
     CDS             complement(4693..6141)
                     /locus_tag="PLS90_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK02486.1"
                     /translation="MHEKLTSKIRVLCVDDDPNVTWILSDGLNAEEFIVETCNDGRAA
                     VNKLSGFKPDVCLLDIKMPGMDGIQLLNYIKRQDPGISVIMISGHADTPIVVQAVQGG
                     ADDFIVKPFDVEMVEICIQKVIEKKDLKQRVQRLESELARQSLWENFIGESPAFLRVR
                     ELIEQISTTDLNVLIRGESGTGKDIVARMIHQLSDRARGPFVKVNCAALPPELLESEL
                     FGHERGAFTGAYRSKPGRFELANKGTIFLDEIGEMAAPVQATLLQVLEHKEFFRVGGT
                     QSVKVDVRLITATNIDIEKHIQEKKFRLDLFYRINDLTVHMPALRERGDDMILLAHHF
                     LEKYGGKFGKGTIDLGQEAERALLQYHWPGNVRELESLIKRAVIFGGGEILKHIERHK
                     DDRAGASESTDGDPFNPEELPTSYFENKTLKEISEGILSQIERRAIKMCLDRQGWNRK
                     KTARDLGISYRCLLYKIQQYDLQKDTVTADSE"
     gene            complement(6196..7944)
                     /locus_tag="PLS90_08515"
     CDS             complement(6196..7944)
                     /locus_tag="PLS90_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02487.1"
                     /translation="MLNCTDPLDPPHLDAGQPEPRPRILIVDDDFDIRLVLSEILRDQ
                     ACEVALAENLSQAREQIEKSPFDTVILDVYLGGDEMGLSLIPWLHERQPNTPVVVISG
                     LANMDDVLVALKTGAYDMLTKPFNNVDVVNGVARAVEKKRMADQNEALLAALRRERDL
                     LEERVRAATREQAETIEMLRLINEQVAAMFELSQAAAADSTTEEVVRRVFELLGRMID
                     FQGGVCVLYDLRARGINLTYSQGERAEGLARIVGRLLAENTDRLILLAEQPNGALTGG
                     LKEHIDAALGPDAPARGLMLMPLYVRQTLLGVVGLIRPHTAMPLDGDDERMLSLAISH
                     LLAALEQRSFIARTAQLSGLGELISEIAHDLRHPMTALRGAARMLIDGWRIDEKRGRC
                     LEELTANLSRMESLVAELVNFYNPREMNMVPVDLHGLLDRALEASSHYIEQGGIELER
                     RYAPGQPMILGLTRNLMEAFINVITNACQAMAGGGKLTVTTEANLNGIHEHRLRGANL
                     QPGGYLCVSIRDTGGGIPEEDHEKVFKRFYTTKPEGHGLGLSAVMRIMRKNLGHVAME
                     SRVGEGTTFHLYLPKT"
     gene            8497..9090
                     /locus_tag="PLS90_08520"
     CDS             8497..9090
                     /locus_tag="PLS90_08520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPK02488.1"
                     /translation="MDESLFTRELAVYNDKLRQAEERIASLEAALQHSRREAIHDRET
                     ALLSSAYFLTRLQEEIVRSERYRHFLSLILIHLQLKNVQSTQELNRELAQIGREMSLG
                     LTRRSDTIALYRRCQMIILLPETDGPGAAHLLERYHATFPSNDRTLSYSVLTYPVDAS
                     NLELVLTRLQDLSEGLYRGTSAVRLSRDPLPSPSPQF"
     gene            complement(9106..10266)
                     /locus_tag="PLS90_08525"
     CDS             complement(9106..10266)
                     /locus_tag="PLS90_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPK02489.1"
                     /translation="MPLVMREDLERLEGELLAPYAQHSAQSRGREAPEPPDPYRTEFQ
                     RDRDRIIHSSAFRKLEYKTQVYVTHEGDFFRTRLTHTIEVAQIARTLAQRLGLNPDLT
                     EAIALAHDLGHTPFGHTGETVLDELMREEGGFEHNAQSLRIVELLEERFHDRPGLNLT
                     WEVRAGIAQHSTAYDRPEITRYHEGSASLESRIADLSDEIAYNNHDIDDALDMGLLHV
                     EQLREVPWVWAIWESVCRDYPADVPPRILKFRALGRLMETMIGDVLTHTSRRLDELRP
                     RSPREAAQCDPRLADFSPAMREGQVQLREFLMRNVYRHPTTLKMQMKAARFLKRLFLL
                     YEESPELLPLKYQSRVAAFGLRRVITDYLSGMTDRYCLEEYMTHFEPTLRRE"
     gene            10455..10952
                     /locus_tag="PLS90_08530"
     CDS             10455..10952
                     /locus_tag="PLS90_08530"
                     /inference="COORDINATES: protein motif:HMM:NF021486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HPK02490.1"
                     /translation="MPRFERHFTLDEANALIPRIRSIFAKVHQLVRDLALPSDVPLPP
                     FGAQAHPPASADTPDPAANGHAGNGNGHGAANVVDIESVWKDLSRHEKMQLINGLLQA
                     VIDDGVVIQDVERGLVDFPAWHGAEEILLCYELADGPQIVAWHDLTSGFAGRRPLDEL
                     FDEPA"
     gene            10979..11659
                     /locus_tag="PLS90_08535"
     CDS             10979..11659
                     /locus_tag="PLS90_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02491.1"
                     /translation="MPQTHWTHPLAGALLLAAGLTLAGCGTAAKQLHADYRALGEEFY
                     TQQMAELDLLSDLLNELGREYFVLQQEYEALGRDQLAQGALRRAQAFHQRHQQLLEMK
                     NGFARKLNQMRRGDLAAVPDAPAAAAPGPPPADPRRVRIVPGAAPGQGAAIQPPARAA
                     QPAPAPPARPVAPSQPLPAPPQDQPSPQAQPSPQPQPAPPAPAPQPAPAQPAAPPAQP
                     AAPPPPGP"
     gene            11715..>12254
                     /locus_tag="PLS90_08540"
     CDS             11715..>12254
                     /locus_tag="PLS90_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HPK02492.1"
                     /translation="MAPLFHRFPRRPPDCPIAPEQCEEPYADAKARMIYEHLWRRGVR
                     DPGVLHAILRVPRERFVPPDLRDEAYEDRALPLAAGQTISQPYIVARMTELARVGPGS
                     LVLEIGAGSGYQTAVLLELGAQVWGIEIVAELADLARDRLIQLGYDRFEILHADGRAG
                     LPDKAPFDAILLAAAPESIP"
BASE COUNT         1857 a         4782 c         3467 g         2148 t
ORIGIN      
        1 aacgtctgat ccgcccccaa cgccgactca aaactcggct ctcggctctc ggctctcgtc
       61 ctcggctctc gaaaaggccg atcaacaatc gcgtgaactc gttgatcgcg atggcttatt
      121 cttagtttcc cggctgcggc ttccgcgctc gtcctttatg tcccttaagt cccttgcgtc
      181 gctgctgtcc cttctgaaat cctctcgccc aggcccttcg ccatgatgcc ttccccgccc
      241 cccgcaacca ccctcccgca acccctcgac cgcccccccg ccggcccgcg ctggtggttg
      301 cgcctgcgcc gccagccctc gttctggatc acctcggccg ccctgctcgc cctcgtcgcg
      361 gtgctctggc tgggacccgc cctcagcccc tacagcgagc gggaacagga tctgacggcg
      421 atttacgccg gccccagcgc gcaacgctgg ttcggcgccg acggcctcgg ccgcgacctg
      481 atgacgcgcg tctttcacgg cggacgcatc tcgctggcgg tcggcgtcgt cggcgccctc
      541 gtcagtctga tcctgggcgt cgcggtcggc ggcgtggccg gctacgccgg cggccgcacc
      601 gagcagctta tcatgcgcct gatcgatttt ctctacggcg tcccgttgct gctggtggtc
      661 atcgccctga tggtggtcac cggccccggt ctggttaata tcttcatcgc gctgggcctg
      721 gtctactggc tcggcatggc gcgcgtcgtg cgggcgcgcg tggcggaact ccgttcgcgc
      781 gattacgtca cggcggcgcg cgccctgggc gccggcccgg cccgcatcct cctgcgccat
      841 gtcctcccca acgccgccgg cgttatcgtc gtcacggcca ccttcatgat cccccaggcc
      901 attttcgccg agtcgttcct cagcttcctc ggcctcggcg tcacgctccc ccacgcctcc
      961 tggggcacgc tggccgccga cggcctcgac gccatgcgct cgcacccgca cgtgctcgcc
     1021 ttcccggccg tcgcgatctg cctgacgatg tttctcttcc aggcgctggg cgaatcgctc
     1081 cgcgcggcgc tcgacccccg cggcgaggat tgaccgctcc gcccccgcgc cgctactctt
     1141 tcagccgcgg cccttcgggt tccgtataca gcgtgatcgt gaatcgcgtc cccacgcctt
     1201 cgcttgagtg ggcttcaatc cgtcccccca tgtcctcgat gaacttgcgc gtcatcgcca
     1261 gccccagccc cgaccccttc gagcccttgg tcgtgaagaa cggcacgaag atccgcggca
     1321 gcacctccgc cggaatcccc ccggcgtcgt cctcaacttc gatctgcgca tgcccctcgt
     1381 cgttgcgcag cgtccgcagc gtcaccgttc ccttgtcctc gggcagcgcc tccagcgcgt
     1441 tgacgatcag gttgaccacc gccttctcca gcccgtccac atccaccagg atccgcggca
     1501 gatcgggcgc cagttccagc cggcagtcga tattccgctt gcgcatctcg tcggcgaacg
     1561 tcgcgcacag ctccgtcaaa aactcgccca ggtcgatcgg ccgtttctcg atcgtccgcg
     1621 cgcgcgagta gtccagcatg tccgccacca gcccgtggat ccgctccagc ccgcgccgca
     1681 ccacccccca tgtctcctcc accgtgtcgt attgcttgcg ctcgagctgc ttgtccatca
     1741 tctccacccc gccctggctc agcagcagga tgttcttgat gttgtgcgcc atccccgtga
     1801 tcgtctgccc gatcgccgcc atccgttcgg tctggatctt gtcctgcacc atccgcaggt
     1861 tgtgcaggtg cgtggccatc tccgcgcaga ccgtcgccag aaacgtcaga tcctcctgcg
     1921 tgaacatccg cactggctcg accgtgtcca gatggcagaa cccgaagaca tgccgcgcgc
     1981 tgatcagcgg cacgcacatc accgagcgca tactgttggc cacgatcgat tcggccccct
     2041 gaaaccgcga atcctccggc gcgttgcgcg tcaggatcgc gatccgttcg cgcaggcccc
     2101 gctgcagaat cgtccggctg atcccgatcg attcgctcgc cagcccgtcg cgcaccttca
     2161 ccgcgcacgg gcggaacacc gcggggtcgt ccttgtcgcg catcagcatc accccgcggt
     2221 cgaccggcag cacctcgaac agcagccgca ggacgttgcg catcagcttc tcctcatcgg
     2281 ccgtcgagcg cagcacctcc tgcagccgat acaggcagat cagccggatc ttcgccttcc
     2341 gcagcgtctc ctcgtcggtg atgtcctcct ccagaatcag atgcggcacc gacgtgtcgt
     2401 cgggcagatc ggcgtggatc accgtctgca ccatgtcgtc ctgctgcacg aaatgcaccg
     2461 ccgaggtctg gtcgctccgc gtgatcaccc gctgatcgct gtcgaactcc tggatcgtca
     2521 gcgagaactc cagactgccg aacgaaatcc cgtcctggtc gacgatcggc gccacctgca
     2581 cccgtttgcc gttgacatag gtcccgttgg acgagcgcag gtcgatgatc cgcggcttgc
     2641 cgccgtagat ctcgatccgc gcgtgatagc gcgagaccgc gtcgaacggc agttcgatgt
     2701 cgttggtggg gtgccggccg atcgccgccg gaaactggtc gatgatatac tccggcttca
     2761 gcccgggaac gagttcccgc ggccgcagcg tcagctgcca gccgccgctc agctcgccgg
     2821 gcgtaaaggt gcgggttggc tgttgctctt tcatcggggg cgaggcggcg cttccgggtt
     2881 cgactgagct cggctcatcg gccgggcctg cactggggag tatgccatcg tgcctgctcc
     2941 gtcaacgccg gccccacggc ggattgtcgc gcagcgcgac gggcggctga aaaatctcgt
     3001 tgagcaccac gacccgccgc atcatcgacg gcgagaagcg aagctgcgcc acggcgcggc
     3061 cgggacggcc cgccggccgc cgcggcgcgg gtcgcggcgc cgccggccgc ggttgccgcg
     3121 ccggctcgac cggaaccgga gcctccgcgc gcggcggctc ctccatcacc ggcatcacgc
     3181 tgatcgcgcg gcgcgccgcc ggcgcggccg gcgccatcgg gcgcggctca ccggcgtcca
     3241 ccggcttgag ccggcgcggc ggcgccgcca ccacgaccgg gcccggccga gccgccgccg
     3301 gcgcatccag cgacgattga cccgccgcgc ggcgctgcgc cgacacatcg gccttgcgct
     3361 gccgctccag ttcctcaatc cgccgctgca gctcgcgctg cacctccatc tcgcgccccg
     3421 tcggcggcgc gtccccctcg tccagcttgc tgtaacgctc gcgcatctcc tgcatcacct
     3481 gctggacctt cttcatgaag gaccagaaga caacgaacac gatgatcagg gtaatcaggc
     3541 cgtccatgag cggttcccgt cggtcagtcg accagcgcct ccagcagcgc cggcggcgcc
     3601 agcgccagca gcgtcgcgcg ccccggcgcg ggaggctctt cgcccagatc cagcgccgcc
     3661 accgctcctt tctccagctc aatcccggcc gccccgccgc cgacaaggca ggcgatcagg
     3721 cgctccagat cgggattgtg ccccaccagc agcaggtcgc cttcgtcgcc gtggcgcccc
     3781 acgcactcca gcatggcttc gggcgatgcg cccggttcca gcgcggcatc caccaccagc
     3841 tcgacgtccc ccttcaacgc cttcgccgcc cggcgcgccg tctccgccgc gcgcggatac
     3901 gggctcgtca ccaccaccgc cggacgccag tccagccggc gcagcgcctt gccggcgatc
     3961 ttgccttgcc ggcgcccccg cttcgtcagc gttcgggccc ggtcatcccc gccgacctcc
     4021 gccgcgtcga cggcctcgcc atgtcgtaga agaaacagct tcatcggggt tccgtgtttg
     4081 cccggcgcct ggtccatgga ccgacccgcg ccgcttcatg ccgttttgct gccggcttca
     4141 tcatccccaa tcggccgcat cgccgtcaac ggaaggatgc gacccgcgca ggccgcgccc
     4201 cccaaccctg ttcgcgccgc ctggccttga atgtgtggca tgccaccaat cgcaagcggc
     4261 ttcaggattc caggttcaat ccaggtgctt ccaactgatc ggttcagtcc gcggagtgga
     4321 cggccgggat aaagcgcagg atgacggccg catgccggta ccctacgtcg ccacccccga
     4381 cgtatcaaac ccctggaagg ggtggccgga gccggtctga atttaaggaa tggcaagcat
     4441 tcagcaggga gcggagtcga ggcagcgttt gtggagtgcg gtgccggagc gttgccgcgc
     4501 aacgcgtcgg caccgctttg gatcggatca cctgggctga tgctgaagag tgaaaatgcc
     4561 cgtcatgcgt cccttatgtc ccttatgtct cttattccgg cgccccaccg cgcccggagt
     4621 cgagctgcat accggcgcca ccgtctaacc accaagcgcg cgccgcccgg ggaccgcata
     4681 ccccttctcc cgtcactcgc tgtccgcggt cacggtgtcc ttctgcaggt cgtattgctg
     4741 aatcttgtag agcaggcagc gatacgaaat ccccaggtcc cgggcggttt tcttgcggtt
     4801 ccacccctgg cggtccagac acatcttgat cgcccgccgt tcgatctggc tcagaatccc
     4861 ctcgctgatc tccttgagcg tcttgttctc gaaatacgag gtcggtaatt cctccggatt
     4921 gaacgggtcg ccgtccgtcg attcgctcgc gccggcccgg tcgtccttgt ggcgctcgat
     4981 gtgcttcagg atctcccccc cgccgaagat caccgcccgc ttgatcaggc tctccagttc
     5041 gcgcacgttc cccggccagt ggtattgcag cagcgcgcgt tcggcctcct gacccaggtc
     5101 gatcgtcccc ttgccgaact tgcccccgta tttctccagg aaatggtgcg ccagcaggat
     5161 catgtcgtcc ccccgctcgc gcagcgccgg catgtggacc gtcagatcgt tgatccgata
     5221 gaacagatcg agccggaatt tcttctcctg gatgtgcttt tcaatatcga tattcgtcgc
     5281 ggtgatcagc cgcacgtcga ccttcacgct ctgcgtcccg ccgacccgga aaaactcctt
     5341 atgctcgagc acctgcagca aggtcgcctg caccggcgcc gccatctcgc cgatttcatc
     5401 cagaaagatc gttcccttgt tggccagctc aaaccgcccc ggcttgctgc gatacgcccc
     5461 ggtgaacgcc cccctttcgt gcccgaacag ctcgctctca agcagctcgg gcggcagcgc
     5521 cgcgcagttg accttcacga acggcccccg cgcccggtcc gagagctgat ggatcatccg
     5581 cgcgacgatg tccttgcccg tcccgctctc cccccggatc agcacgttca aatcggtcgt
     5641 gctgatctgc tcgatcaatt cccgcacgcg caggaacgcc ggcgattcac cgatgaaatt
     5701 ctcccacagc gactgccgcg ccagttccga ctccagacgc tgcacccgct gcttcaaatc
     5761 cttcttctcg attaccttct ggatgcagat ctcgaccatc tccacgtcga acggcttgac
     5821 gatgaagtcg tcggcccccc cctgcaccgc ctgcaccacg atcggcgtgt cggcgtgccc
     5881 cgaaatcatg atcaccgaga tccccgggtc ctgccgcttg atgtagttga gcagttgaat
     5941 cccgtccatc cccggcatct tgatgtccag caggcagacg tcgggcttga accccgacag
     6001 cttgttcacc gcggcgcgtc cgtcattgca ggtctcaacg atgaattcct cggcgttcag
     6061 gccgtccgac agaatccacg tgacgttggg atcgtcgtcg acacacagga ctcggatctt
     6121 gctcgtcagc ttctcgtgca tcgggtacac ccttccaatc gacaggtcaa acgcgcctcc
     6181 gccggcgccg ccgcgtcagg tcttcggcag atacagatga aatgtcgtcc cctcgccgac
     6241 gcgactctcc atcgccacgt gccccaggtt cttgcgcatg atccgcatca ccgccgacag
     6301 ccccaggccg tgaccctccg gcttcgtcgt atagaaccgc ttgaacacct tctcgtggtc
     6361 ttcctccgga atgcccccgc ccgtgtcgcg gatcgacacg cacaggtagc cccccggctg
     6421 caggttcgcc ccccgcaacc ggtgctcgtg gatcccgttc aggttcgcct cggtcgtcac
     6481 cgtcaacttg ccgccgccgg ccatcgcctg gcaggcattg gtgatcacgt tgatgaacgc
     6541 ctccatcagg ttgcgcgtca gccccagaat catcggttgc cccggcgcgt agcggcgctc
     6601 cagttcgatc cccccctgct caatgtaatg cgaactcgcc tccagcgctc ggtccagcag
     6661 cccgtgcagg tccaccggca ccatgttcat ctcgcgcggg ttgtagaagt tcaccagttc
     6721 ggccaccagg ctttccatcc gcgacaggtt tgccgtcagt tcctccaggc accgcccgcg
     6781 tttctcgtcg atccgccacc catcgatcag catccgcgcc gccccgcgca gcgccgtcat
     6841 cgggtggcgc aggtcgtgcg cgatctctga aatcagttcc cccagccccg aaagctgcgc
     6901 ggttcgcgcg atgaagctgc gctgctccag cgccgccagc agatgcgaga tcgccagcga
     6961 caacatccgc tcgtcgtccc cgtcgagcgg catcgccgta tgcggccgga tcagtcccac
     7021 cacccccagc agcgtctggc gcacgtagag cggcatcagc atcagcccgc gcgccggtgc
     7081 gtcgggcccc agcgcggcgt cgatgtgctc cttcagcccc ccggtcagcg cgccgttggg
     7141 ctgctcggcc agcaggatca gccggtcggt gttctcggcc agcagccgcc cgacgatccg
     7201 ggccaggccc tcggcccgct ccccctggct gtacgtcagg ttgatcccgc gcgcgcgcag
     7261 atcgtacagc acgcacacgc ccccctgaaa atcgatcatc cggccgagca gctcgaaaac
     7321 ccgccgcacg acctcctccg tcgtgctgtc ggccgccgcc gcctggctca gctcgaacat
     7381 cgccgcgacc tgttcattaa tcagccgcag catctcgatc gtctcggcct gctcgcgcgt
     7441 cgccgcgcgc acccgttcct ccagcaggtc gcgctcccgg cgcagcgccg ccagcagcgc
     7501 ctcgttctga tccgccatcc gcttcttttc aaccgcccgc gccaccccgt tgaccacgtc
     7561 cacgttgttg aacggcttgg tcaacatgtc gtacgcgccc gtcttgagcg ccaccagcac
     7621 gtcgtccatg ttcgccagac ccgaaatgac gaccaccgga gtattgggct ggcgctcgtg
     7681 cagccagggg atcagcgaca gacccatctc gtccccgccg agatagacgt ccagaatcac
     7741 cgtatcgaac ggcgactttt cgatctgctc gcgcgcctgg ctgaggttct ccgccagcgc
     7801 cacctcgcac gcctgatcgc gcaggatctc gctcagcacc agccggatgt caaaatcgtc
     7861 gtcgacgatc agaatgcgcg gccgcggctc cggctggccg gcatccagat gcggagggtc
     7921 cagggggtcg gtgcagttga gcatggcgag gcgaattctc cccggttgcg ccggcagcgg
     7981 tggggccgta accggaacgg acaggtgcat actctagcat cacggcccgc cccggatgca
     8041 agcggactca cttctatgca tcggcgttca caccctcaaa ctgaagatct gaggcaaaaa
     8101 gggtgcaaaa gcttgctgtt taatgctgca aattgttgca tgcgccgggg aagaccacca
     8161 ccccaattcg ccgcgggccg ccctgaatct tatcttgatt atcggatcac gaatactgaa
     8221 agtggtctcg catcggggtt ttcgcccctc cgccgcttcc gcgccccctc ggcgccctta
     8281 cccggttggt tggcatcagg attgcatgcc tccaccacgt cattcagcag ggctgctgag
     8341 cgtgccgctt gaactctggc cggttttggc tgcgcggtcg ccgtccgacg ggctcccccg
     8401 atgactgctt cgcatgccgc ttccgctgat cgctggccct cgccgacggc ccccctgacc
     8461 ccttcggcga ctcaacccgt tgctcgtgct gacgcgatgg acgaatccct cttcacccgc
     8521 gaactggccg tctacaacga caaactccgc caggccgagg agcgcatcgc ttccctcgag
     8581 gccgcgctcc agcattcccg ccgcgaggcg atccacgacc gcgaaaccgc cctcctctcc
     8641 tccgcctatt tcctcacccg cctccaggag gaaattgtcc gttccgagcg ctaccgccat
     8701 ttcctcagcc tgatcctgat ccacctccaa ctcaaaaacg tccagtccac ccaggaactc
     8761 aaccgcgaac tggcccagat cggccgcgaa atgagcctcg gcctcacccg ccgcagcgac
     8821 accatcgccc tctaccgccg ctgccagatg atcatcctcc tccccgaaac cgacggcccc
     8881 ggcgccgccc acctcctcga acgctaccac gccaccttcc ccagcaacga ccgcaccctc
     8941 tcctactcgg tcctcaccta cccggtcgac gccagcaacc tagaactggt tctgacccgc
     9001 ctgcaggacc tctccgaggg actctaccgc ggcacgagcg ccgtccgcct ctcccgcgac
     9061 ccgctccctt ccccttcgcc ccagttctga ccccgccccg cccggttatt cgcggcgcaa
     9121 cgtcggctcg aagtgcgtca tgtactcctc caggcagtag cggtcggtca tccccgacag
     9181 gtaatccgtg atcacccggc gcagaccgaa cgccgccacg cgcgactggt atttcagcgg
     9241 gagcagctcg ggcgactcct cgtagagcag gaacagccgc ttcagaaacc gcgccgcctt
     9301 catctgcatc ttcagcgtcg tcgggtgccg gtagacgttc cgcatcagaa actcgcgcaa
     9361 ctgcacctgc ccctcgcgca tcgccgggct gaaatcggcc aaccgcgggt cgcactgcgc
     9421 ggcttcccgc ggcgaccgcg gccgcagctc gtccagccgc cggctggtgt gcgtcagcac
     9481 gtcgccgatc atcgtctcca tcagccgccc cagcgcgcgg aatttcaaga tccgcggcgg
     9541 cacgtccgcc ggatagtccc tgcacaccga ctcccagatc gcccacaccc agggcacctc
     9601 gcgcagctgt tccacatgca acagccccat atcgagcgcg tcatcgatgt catggttgtt
     9661 gtaggcgatc tcgtccgaca ggtcggcgat ccgcgattcc agcgacgcgc tcccctcgtg
     9721 atatcgcgtg atctcgggcc ggtcatacgc cgtcgaatgc tgcgcgatcc ccgcccgcac
     9781 ttcccacgtc aggttcagcc ccggccggtc atgaaaccgc tcttccagca gctcgacgat
     9841 ccgcaggctc tgcgcattgt gctcgaaccc tccctcctcc cgcatcagtt catccagcac
     9901 cgtctcgccg gtgtgcccga acggcgtgtg gcccagatcg tgcgccagcg cgatcgcctc
     9961 ggtcaggtcg ggattcaacc ccagccgctg cgccagcgtg cgggcgatct gcgccacttc
    10021 gatcgtgtgc gtcagccgcg tgcggaaaaa atccccctcg tgcgtcacgt agacctgcgt
    10081 cttgtattcc agcttgcgga acgccgagga gtggatgatc cggtcgcggt cgcgctgaaa
    10141 ttccgtccgg tacgggtccg gcggctcggg cgcctccctc ccgcgcgatt gcgcgctgtg
    10201 ctgcgcatac ggcgccagca gttccccttc cagacgttcc agatcctcac gcatcaccag
    10261 cggcatcgat cccggttccc cccaggggtc ttgcggccgt cccgaccggc gccttccacg
    10321 ttggcacaag ggggggccac agacaattga caactgattg aggaaacggc ggcaaatctg
    10381 ttaagaagga cggcggccgc gcggcggacg agccccgccc ccgcgccgcg caagtcccct
    10441 gatcggattt ccccttgccg cgcttcgaac gccatttcac gctcgacgaa gctaacgcgc
    10501 tgatcccgcg catccgctcg atcttcgcca aggtccacca gctcgtgcgc gacctggcgc
    10561 tgccgagcga tgtcccgctc ccccccttcg gcgcccaggc ccaccccccg gcgtccgccg
    10621 acacgcccga ccccgccgcc aacggccacg cgggcaacgg caacggccac ggcgcggcca
    10681 acgtcgtcga tatcgaatcg gtctggaaag atctgagccg ccacgagaag atgcaactga
    10741 tcaacggcct gctccaggcg gtcatcgacg acggcgtcgt catccaggac gtcgaacgcg
    10801 gcctggtcga tttccccgcc tggcatggcg cggaggaaat ccttctctgt tacgaactgg
    10861 ccgacggtcc ccagatcgtc gcctggcacg atctgacctc gggcttcgcc ggccgccggc
    10921 cgctcgatga actcttcgac gaacccgctt gaaatcatcc cggagaacgc ccctcacgat
    10981 gcctcaaacg cactggacgc accccctcgc gggcgcgctg ctgctggccg ccggcctgac
    11041 cctggccggc tgcggcaccg ccgccaaaca gcttcacgcc gactatcgcg ccctcggcga
    11101 ggaattctac acccagcaga tggccgagct ggatctgctc agcgacctgc tcaacgaact
    11161 gggccgcgaa tacttcgtcc tgcagcagga atacgaggcc ctcggccgcg accagctcgc
    11221 ccagggcgcc ctgcgccgcg cccaggcgtt ccatcagcgc caccagcaac tgctcgagat
    11281 gaaaaacggc ttcgcccgca agctcaacca gatgcgccgc ggcgacctgg ccgcggtccc
    11341 cgacgccccg gccgcggccg ccccgggccc gcccccggcc gatccgcggc gcgtccgcat
    11401 tgtccccggc gcggcgcccg gccagggcgc cgcgatccag cccccggcgc gcgccgccca
    11461 gccggccccc gcgccccctg cccggccggt ggcgcccagc cagcccttgc ccgccccgcc
    11521 gcaggatcag ccgtcgccgc aggctcagcc gtcgccacag ccgcaaccgg ccccgccggc
    11581 ccccgcgccg caaccggccc cggctcagcc cgctgcaccc ccggctcaac ccgccgcgcc
    11641 gccgcccccc ggcccctgac cgccgcgcgg aagggtggcg cgatgctcgc ttagcccctc
    11701 ggctggagac ccccatggcg ccgctcttcc atcgattccc gcgccggccc cccgactgcc
    11761 cgatcgcccc cgagcaatgc gaggagccct acgccgacgc caaggcgcgc atgatctatg
    11821 aacatctctg gcggcgcggc gtccgcgacc ccggcgtcct gcacgccatc ctccgcgtcc
    11881 cgcgcgaacg cttcgtgccc cccgacctgc gcgacgaagc ctatgaagac cgcgctctcc
    11941 ccctcgccgc cggccagacc atctcccagc cctacatcgt cgcccgcatg accgaactgg
    12001 cccgcgtcgg ccctggctcc ctcgtcctcg aaatcggcgc cggctcgggc tatcagaccg
    12061 ccgtcctgct cgaactgggc gcccaggtct ggggcatcga gatcgtcgcc gagctggccg
    12121 accttgcccg cgaccggctc attcaactcg gctacgaccg gtttgaaatc ctccacgccg
    12181 acggacgcgc cggcctgccc gacaaggccc cgttcgacgc gatcctgctc gccgccgccc
    12241 ccgaatccat cccc
//
LOCUS       DAOPCO010000100        12114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5208_length_12114_cov_5.143627, whole genome shotgun sequence.
ACCESSION   DAOPCO010000100 DAOPCO010000000
VERSION     DAOPCO010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_12114_cov_5.143627"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /gene="rplC"
                     /locus_tag="PLS90_08545"
     CDS             complement(<1..133)
                     /gene="rplC"
                     /locus_tag="PLS90_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012164967.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPK02493.1"
                     /translation="MTIGLLGKKLGMTRIYDENGTIIPVTVIQAGPCPIIQVKTAQKD
                     "
     gene            complement(253..567)
                     /gene="rpsJ"
                     /locus_tag="PLS90_08550"
     CDS             complement(253..567)
                     /gene="rpsJ"
                     /locus_tag="PLS90_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPK02494.1"
                     /translation="MARQQKIRINLKAFDHRLLDQSVSEICDTARRNGARIAGPIPLP
                     TRISKYTVLRSPHVDKKSREQFEMRRHKRLIDILEPNQKTLDALTELDLPAGVYVEIK
                     LT"
     gene            complement(648..1838)
                     /gene="tuf"
                     /locus_tag="PLS90_08555"
     CDS             complement(648..1838)
                     /gene="tuf"
                     /locus_tag="PLS90_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPK02495.1"
                     /translation="MAKEAFKRTKPHVNVGTIGHIDHGKTTLTAAITLTQANRGFGQA
                     VAFDQIDKAPEEKARGITISTAHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPYIVVYLNKCDAVDDPELLELVELEVRELLDK
                     YEFPGDETPIIRGSALEALEGKDTEYGAQSIARLMDAIDSYIPEPQRPLDQPFLMPVE
                     DVFSITGRGTVATGRIERGMVKVGETVELVGIRPTATTVVTGVEMFRKTLDQGQAGDN
                     VGLLLRGVDKEGIERGQVICKPKSITPHTHFKANVYVLTKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGEVILPEGVEMVMPGDNIELEGKLISPIAMEDGLRFAIREGGRTVGAGVVS
                     KIIE"
     gene            2667..6206
                     /locus_tag="PLS90_08560"
     CDS             2667..6206
                     /locus_tag="PLS90_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK02496.1"
                     /translation="MSQSVPTPAPPAPAGAGRGFWSLVILQFQGALSDNIFRISLMLM
                     ASSILTDSSAQFGKYNALLGMLFPISYLLFSVWAGFLADRYSKRTVTLATKLAEVVLM
                     LLAVGAFLSVGNLETATPRSLALPTVVLFLMFSQSAFFSPAKYAIIPELVPEHRLGWA
                     NGVIGLWTYLAIILGATVATGLVPLLKPNLLTGIPMLLVVLAVVGYVIGLRIARVPAA
                     NPQRPILTNPLPELRGYFGVIFKDRALTLTVLGLAYFWALGSVLLVHVLVWSRDSMGY
                     GDQGQGAHFLLLALGIGIGSALAGALSRNRIEVGLIPVGALMMAAFAFPLALASPDNR
                     VAMAICLALVALGGGIFSVPLNALLQQLTDPRDRGSLLAANNYVTNVAMLFATFLYWL
                     ANKAEISPNGIFVYVGFITLCGTALVLFFTPEALVRFAGYALTRTIYRIRVTGVEHIP
                     ASGPALIVSNHVSFVDALLLMAVTPRPIRFIAHESFFGKAFIGMFLRATRSIPVSASQ
                     GPRELIRSMKTAAEALRQGEIICIFIEGQITRTGMMLPFRRGYEFILKEAPAPLIPVY
                     LYGVWGSIFSFSEGRFFWKWPRQVPYRVAVRFGAPLPHNVDPYRLRLILQELGADSAI
                     AAKPDLPLVHHRMVASARREWRRFSMVDSVSTDPVSLGGLLWRTLVVARKLRPIWQGQ
                     ERVGILLPPGIGGALVNFAAAFTGRTVINLNYTVGQDVLNSCAERTGMRTIITSLKFM
                     ERLKLECPVPAVYMEDLRTRPPLLEVIKCILAARFLPARRLEKFCGAIRHPVPDDPLT
                     IIFSSGSTGLPKGVVLSHFNVTANLESFKQGVRFYPSDRLLGVLPFFHSFGYTVSLWG
                     AVGIPIGAVYHYNPLDVKTIGEMCQRHKVTVIVSTATFMQHYIRRIAPEQFGGLNAVV
                     AGAERLPESTRLRFLEKFGVDPLQGYGTTELSPVVSVNVDHYRSPGFYQVGHKPGSIG
                     HPIPGVAVQVRDPETGEPRGVNEEGLLWVKGPNVMQGYLGMPEKTAEVLRDGWYNTGD
                     MARIDEDGFIFITGRLSRFSKIGGEMVPHGKVEDAISEVLKLEETQVAVTGVPDEKKG
                     EQLVVLHTLDEAQLEGVSEKLSAAGLPNLFIPRREFFFKVEALPLLGTGKLDLQGVQA
                     LARQLAAERAANGSNGDEVAANAGEKDGEAGRA"
     gene            6297..6722
                     /locus_tag="PLS90_08565"
     CDS             6297..6722
                     /locus_tag="PLS90_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPK02497.1"
                     /translation="MSVYQHEIVVRTAGHGDMHDLTDEVATLVGQSGIERGVAHVFNI
                     GSTAAIGTIEFEPGLQRDLPDILDKLMPPSRAYGHEQAWHDGNGHSHLQATVLGPELT
                     VPIRDGRLLLGTWQQIFHLECDVKPRERRVVVTILGEPA"
     gene            6740..7066
                     /locus_tag="PLS90_08570"
     CDS             6740..7066
                     /locus_tag="PLS90_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPK02498.1"
                     /translation="MTLAECQRAVDDWIRTVGVRYFSELTNMAVLTEEVGELARLMAR
                     QYGDQSFKPGEEQLDLGEELADVLFVLICLANQTGVDLEQAFEKTMEKKSSRDRDRHR
                     RNEKLS"
     gene            complement(7407..8120)
                     /locus_tag="PLS90_08575"
     CDS             complement(7407..8120)
                     /locus_tag="PLS90_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HPK02499.1"
                     /translation="MAIFAAGAVAVFAATVLIVTAAPPNPAGTRLADDAALQAETPTP
                     ALSMSLAEYLRQRLGRRFHEREEWTGGHAFPPQPERDRTWEPIGAAKFITIHHTGAIS
                     NNPPAELIRSIYRDHTRPHSRLDAADVGYHFFVDSRGEVWEGRIVGRKGTHVGSTPDG
                     RNNAGNIGICGLGNFVSRRPPEPMVSSVVDLAELIQEYYGRKMTIRGHRDWRGINGFA
                     STDCPGLLEDAVRLARLRQ"
     gene            complement(8687..9079)
                     /gene="pspC"
                     /locus_tag="PLS90_08580"
     CDS             complement(8687..9079)
                     /gene="pspC"
                     /locus_tag="PLS90_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HPK02500.1"
                     /translation="MKHRSYAGRPRLFRSSTDRMLLGVCGGLAEHFDFSPAGVRLLAV
                     ILFIFSGFFPLILLYIVLGVILPPAPLRPAFESEQEEELWDLYHASRGAAMGKLRQRF
                     QALDRRLQRIESIVTSPEFELEEEYRRL"
     gene            complement(9234..9668)
                     /locus_tag="PLS90_08585"
     CDS             complement(9234..9668)
                     /locus_tag="PLS90_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02501.1"
                     /translation="MLKLIGIIALVMLGVWLLGGLVAGVLGGLVGLVAGLLSFVFGIV
                     GFVLKLVGGLVLLIVKAGLTLVLLGVALLAIGGLILRALFRRGDRPRAAAAWRSHYGH
                     SMRRRDDIHAAMDESLRRAEERMARLEGVLAERMRRRHFEPW"
     gene            complement(9736..10014)
                     /locus_tag="PLS90_08590"
     CDS             complement(9736..10014)
                     /locus_tag="PLS90_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02502.1"
                     /translation="MEPIIPLAAILMPVMLFAIAALGPLLLIALIVWVGFRVVRGGNR
                     REESLNAQRAAQDELRALMSGMEKMERRIENLETILLERGRSGAAERL"
     gene            complement(10309..10995)
                     /gene="pspA"
                     /locus_tag="PLS90_08595"
     CDS             complement(10309..10995)
                     /gene="pspA"
                     /locus_tag="PLS90_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HPK02503.1"
                     /translation="MGIFTRFRDIVSANLNAILDRAEDPEKMVRLMIQEMEDTLIEVK
                     SNCAGVLAEQKKLERALKGARREAADWEAKAGLALQKQREDLARAALAEKRRHEQRVR
                     SLEEELDRTRENVASFKDDIAQLEAKLNDAREKQRTIIQRRQAAESRFRTAAHVRRVD
                     TTEAFARFEAYERGIDRLEAEADLVDSLRPRRPTLRDELADLEYSDEIEQELERLKEQ
                     LRSGSAGRNA"
     gene            11236..>12114
                     /locus_tag="PLS90_08600"
     CDS             11236..>12114
                     /locus_tag="PLS90_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK02504.1"
                     /translation="MSGTSPDPPQALGESEAFLALQERISQAARTDRPVILIGERGTG
                     KELAAARLHLLSARWGQPYVTLNCASLAPTLIESELFGHEAGAFTGAVRLRKGRFEIA
                     DGGSLFLDEVANIPPTVQEKLLRTVEYGQMERVGSSETRRVDVRLIAATNVDLRAEAE
                     AGRFRRDLLDRLAFEVIVLPPLRRRREDIVLLARHFARRMAGQLGLEFQPEFSPAARQ
                     ALLAHPWPGNVRELRNVVERMVYRTRARLIEEIVFDPFDSPDMPGGGPGPAGNARAAS
                     SPEAAAGLAELNALPLP"
BASE COUNT         1957 a         3767 c         4201 g         2189 t
ORIGIN      
        1 cgtccttctg ggccgttttg acctggatga tcgggcaggg gccggcctga atgacggtca
       61 cgggaatgat cgtcccgttc tcatcataga tgcgcgtcat gccgagcttt tttccaagca
      121 gtccaattgt cattcccgga tgctcctctt ggcgggaagg cgatctcggc gccgacggcc
      181 ttgtccgtcc caccgattgg ccccagcccg gcgtcgccga tcacggcagg gcccgcgcct
      241 tcactcgtcc gctcaagtca gcttgatttc cacatagacc cccgccggca gatcgagctc
      301 cgtcagggca tcgagcgtct tctgattcgg ttccaagata tcaatcagcc gcttgtggcg
      361 gcgcatctcg aactgctcgc gcgacttttt gtcgacgtgc ggcgagcgca gcacggtgta
      421 tttgctgatc cgcgtcggga gcggaatcgg cccagcgatg cgggccccgt tgcgccgtgc
      481 cgtgtcgcag atctcgctga ccgactgatc gagcaaccga tggtcaaagg ccttcaggtt
      541 gatgcggatc ttctgctgtc gcgccatgtc ctggttccac ctaccgagcg tcttcgcgtc
      601 gttacaaagg ttcgccgccc gagggcgagc catcgggcgg ccgtgggtta ttcgatgatc
      661 ttgctgacca cgccggcgcc gacggtgcgg ccgccctcgc ggatggcgaa gcgcaggcca
      721 tcctccatcg cgatcgggct gatcagcttg ccctcgagct cgatgttgtc gcccggcatc
      781 accatttcca ccccttcggg caggatcacc tcgcccgtca cgtcggtcgt gcggaagtag
      841 aactgcggcc ggtagccctt gaagaacggc gtgtggcggc ccccctcctc cttggtcagc
      901 acgtagacgt tggccttgaa gtgggtgtgc ggcgtgatcg acttgggctt gcagatcacc
      961 tggccgcgct cgatcccctc cttgtcgacg ccgcgcagca gcaggccgac attgtcgccc
     1021 gcctggccct ggtccagcgt cttgcggaac atctcgacgc cggtgacgac cgtcgtcgcg
     1081 gtggggcgga tgccgaccag ttcgaccgtc tcgccgacct tgaccatgcc gcgctcgatg
     1141 cggccggtgg cgaccgtgcc gcgccccgtg atcgagaaga cgtcctcgac cggcatcagg
     1201 aacggctggt ccagcgggcg ctgcggctcg gggatgtagc tgtcgatggc gtccatcagc
     1261 ctggcgatcg actgcgcccc gtactccgtg tccttgccct cgagcgcctc gagcgcgctg
     1321 ccgcggatga tcggcgtctc gtcgccggga aactcatact tgtcgagcag ctcgcgcacc
     1381 tccagctcga ccagctcgag cagctcgggg tcgtccaccg cgtcgcactt gttcaggtag
     1441 acgacgatgt acgggacgcc gacctggcgc gccagcagga tgtgctcgcg cgtctggggc
     1501 atcgggccgt cggtcgccgc gacgacgagg atcgcgccgt ccatctgcgc cgcgccggtg
     1561 atcatgttct tgatgtagtc ggcgtggccc gggcagtcga cgtgcgcgta gtggcggttg
     1621 gcggtctcat actcgacgtg cgcggtcgaa atggtgatgc cgcgcgcctt ctcctcgggg
     1681 gccttgtcga tctggtcgaa cgccacggcc tggccgaaac cccggttggc ctgcgtcagc
     1741 gtaatcgccg ccgtcagcgt cgtcttgccg tggtcgatgt gaccgatcgt ccccacgttc
     1801 acgtgcggct tggtgcgctt gaatgcttcc ttggccatgt tgcatctccc acgtaagggt
     1861 atctggacgg ctggcttcga cgggaatcga agagaccgtc gggcgccgcg gctccggacg
     1921 gggaagccgg caacacctgc aaagactgga ggctgaagcg aggagggcga gggtcttgcg
     1981 cgccggcgcc ggtcaagccc aggggggcca ccagcagccg cggtcgcgaa ggagccctcg
     2041 cttcttgcat ctcaaccact tcagcgagcg ggtcgatcca aaccagcgga ccggacgatc
     2101 cgccgcccca gtgaggggac tggggccgcc gccttaacct ggccgcgaac atatttcgtc
     2161 cgtgagcagg tcacaaagtt tggcattgta gcgcaatggg tggcgaagtc aagctggaaa
     2221 ggcgcacttt tttcgcggcc gtcacccggg tgggaaaggc gcgtgccgtc gacctccccc
     2281 gccgatgcca aaccgcccat atgtagcatc gaacggcggg cggttcaagg gggaaaagag
     2341 tctttataga tgtggttggt gaaccggggg ttcggaacaa aggggatgga aatggaggtt
     2401 ttcgagagcc gaggatgagg gccgagagcc gagagccgaa ttctggagag gggcttcggg
     2461 agcgctcaca aggacgatga tgagggacgc gggacgtaat cttgcgggag ggcatcaagg
     2521 acgcaaagga catatagggg tcctcgaacg ggggtttcaa tttcagttgc agggattgcg
     2581 ccggggtcgg caagatgacg aacccgtcgt ccatgccgga taaccgtctg agcaatcagg
     2641 aatttcacat tgaagcgaac gcgcccatga gtcaaagcgt ccccaccccc gccccccccg
     2701 cgccggcggg cgccggccgc ggtttctggt cgctggtgat cctgcaattc cagggggcgc
     2761 tgagcgacaa catcttccgc atctccctga tgctgatggc gagctcaatc ctgaccgatt
     2821 cgtcggcgca gttcggcaaa tataacgccc tgctggggat gttgttcccg atctcctacc
     2881 tgctgttctc ggtctgggcg ggttttctgg ccgatcgcta cagcaagcgg accgtgacgc
     2941 tcgccaccaa actggccgaa gtggtgctga tgctcctggc cgtcggcgct ttcctgagcg
     3001 tcgggaatct ggagacggcg acgccgcgct cgctggcgtt gcccacggtc gtgctgttcc
     3061 tgatgttcag ccagagcgcg ttcttcagcc cggccaagta tgccatcatc cccgaactcg
     3121 tgccggagca ccggctgggc tgggccaacg gggtcatcgg cctgtggacc tatctggcga
     3181 tcatcctggg cgcgacggtc gcaacaggcc tggtgcccct gctcaagccg aatctgctga
     3241 cggggatccc gatgctgctg gtcgtgctgg ccgttgtcgg ctacgtgatc ggcttgcgga
     3301 tcgcccgggt gccggccgcc aacccgcaac ggccgatcct gaccaaccca ctgccggagc
     3361 tgcgcggcta cttcggcgtg attttcaagg accgcgcctt gacgctgacg gtgctcggcc
     3421 tggcctactt ctgggcgctc ggctcggtgc tgctggtgca cgtgctggtc tggagtcgcg
     3481 attcgatggg ctacggcgac cagggccagg gggcgcactt cctgctgctg gcgctgggca
     3541 tcggcatcgg ttcggcgctg gcgggggcgc tcagccgcaa tcgaatcgag gtcggcctga
     3601 tccccgtcgg ggcgctgatg atggcggcct tcgcctttcc gctggcgctg gcgtcgccgg
     3661 ataaccgggt ggccatggcg atctgcctgg cgctggtggc cctcggcggg ggcatcttct
     3721 ccgtgccgct caatgcactg ctgcagcagt tgaccgaccc gcgcgaccgg gggagcctgc
     3781 tcgccgcgaa caattacgtc accaacgtcg ccatgctgtt cgccacattc ctgtactggc
     3841 tggcgaacaa ggccgagatc tcgcccaacg ggatcttcgt ctacgtcggt ttcatcacgc
     3901 tgtgcgggac ggccctggtg ctcttcttca cgcccgaagc gctggttcgc ttcgccggct
     3961 acgccctgac ccggacgatc taccgcattc gcgtcaccgg cgtcgaacac atcccggcgt
     4021 cgggcccggc gctgatcgtg agcaaccatg tctcgtttgt ggacgcgctg ctgctgatgg
     4081 cggttacgcc ccggccgatc cggttcatcg cgcacgagag cttcttcggc aaggcgttca
     4141 tcggaatgtt tctgcgggcg acgcgcagca tcccggtcag cgcctcgcag ggtccgcgcg
     4201 agctgatccg ctcgatgaaa accgccgccg aagcgctccg tcagggcgag atcatctgca
     4261 ttttcatcga agggcagatc acgcgcacgg ggatgatgct ccccttccgg cgcggctatg
     4321 agttcatcct gaaggaagcg ccggctccgc tcatcccggt ctacctctat ggcgtctggg
     4381 gcagcatttt cagtttttca gagggccggt tcttctggaa atggccgcgg caggtgccgt
     4441 atcgcgtcgc cgtgcggttc ggcgcgccgc tgccgcacaa tgtcgacccc tatcgcctgc
     4501 gcctgatcct gcaggaactg ggggcggaca gcgccatcgc cgcgaagccg gacctgccgc
     4561 tggtgcatca ccggatggtg gccagcgcgc ggcgcgagtg gcggcggttc agcatggtcg
     4621 attcggtgag caccgatccc gtgagcctgg gcgggctgct gtggcgcacg ctggtcgtcg
     4681 cgcgcaagct gcgcccgatc tggcaggggc aggagcgcgt cgggatcctg ctgccgccgg
     4741 ggatcggcgg ggcgctggtg aacttcgcgg cggcctttac ggggcgcacc gtcatcaacc
     4801 tgaactacac ggtcgggcag gatgtcctca attcgtgcgc cgagcgcacc ggcatgcgga
     4861 cgatcatcac ctcgctcaag ttcatggagc ggctcaagct ggagtgcccg gtgccggcgg
     4921 tctacatgga ggatctgcga acccggccgc cgctgctcga ggtcatcaag tgcattctgg
     4981 cggcgcggtt cctgccggcg cgccggctgg aaaaattctg cggagcgatc cgccacccgg
     5041 ttccggatga tccgctgacg atcatctttt ccagcggctc gaccggactg cccaaggggg
     5101 tcgtgctgag ccacttcaac gtgacggcca acctggaaag cttcaagcag ggcgtgcggt
     5161 tctaccccag cgaccggctg ctgggggtgc tgccgttctt ccacagcttc ggctacaccg
     5221 tctcgctgtg gggggcggtg ggcattccga tcggcgcggt ctatcattac aacccgctcg
     5281 acgtgaagac gatcggcgag atgtgccagc gccacaaggt gacggtgatc gtcagcacgg
     5341 cgacgttcat gcagcactac atccggcgga ttgcgccgga acagttcggc gggctgaatg
     5401 ccgtcgtcgc cggcgccgag cggctgccgg agtcgacgcg gctgcgcttc ctggagaagt
     5461 tcggcgtcga cccgctgcag ggttacggca cgacggagct gtcgccggtc gtttcggtca
     5521 atgtcgatca ttaccgttcg cccggtttct atcaggtcgg tcacaagccc ggttcgatcg
     5581 ggcatccgat cccgggcgtg gcggtgcagg tgcgcgatcc cgagacgggc gaaccgaggg
     5641 gggtgaacga agaggggctg ctgtgggtga aggggcccaa cgtgatgcag ggctacctgg
     5701 ggatgccgga gaagacggcc gaggtcctgc gcgacggctg gtacaacacc ggcgacatgg
     5761 cgcggatcga cgaggacggg ttcattttca tcaccggccg gttgtcgcgc ttcagcaaga
     5821 tcggcggcga aatggtgccg cacggcaagg tcgaggacgc catcagcgaa gtgctgaagc
     5881 tggaagagac acaggtggcg gtgaccggcg ttcccgacga gaagaagggc gaacagctgg
     5941 tagtgctgca cacgctggat gaagcgcagc tggaaggagt gagcgaaaag ctgagcgccg
     6001 ccgggctgcc caacctgttc atcccgcggc gcgagttttt cttcaaggtg gaggcgctgc
     6061 cgctgctggg gacgggcaag ctcgacctgc aaggggtgca ggcgctggcg cggcaactgg
     6121 cggccgaacg cgccgccaac gggagcaatg gggacgaagt ggcggccaac gcgggagaga
     6181 aggatggcga ggccgggcgg gcgtgatcgc gatgcatcgg ccgagcatgg cgaagatcgt
     6241 ttcgaacaga cccgcagact gagcggagaa gcccggcaac gggggggagt ccagcgatga
     6301 gcgtgtacca gcacgaaatc gtggtgcgga ccgctggcca cggcgacatg cacgacctga
     6361 ccgacgaggt cgcgacgctc gtcggccaga gcgggatcga gcgcggcgtg gcgcatgtgt
     6421 tcaacatcgg gtcgacggcg gcgatcggga cgatcgaatt cgaaccgggg ctccagcgcg
     6481 acctgcccga catcctcgac aaactgatgc cgcccagccg cgcctacggg cacgaacagg
     6541 cctggcacga cggcaacggc cacagccacc tgcaggcgac ggtgctcggg ccggagctga
     6601 ctgttccgat tcgggacggc cggctgctgc tgggcacctg gcagcagatc tttcatctgg
     6661 aatgcgacgt gaagccgcgc gagcggcggg tcgtcgtcac catcctggga gagccggcat
     6721 gacgcgggag ggcaaggcgc tgacgctggc ggagtgccag cgggcggtcg acgactggat
     6781 ccggacggtc ggggtgcgct acttttccga gctgaccaac atggcggtgc tgacggaaga
     6841 ggtgggcgaa ctggcgcggc tgatggcgcg gcaatacggc gaccagagct tcaagccggg
     6901 cgaggagcag ctcgacctcg gcgaggaact ggccgatgtg ctgttcgtgc tgatctgtct
     6961 ggcgaatcag acgggggtcg atctggaaca ggcgtttgaa aagacgatgg agaagaaaag
     7021 tagccgggac cgggaccggc atcgaaggaa tgagaagctg agttagggga aattcgaagc
     7081 tcgaaccacg aaattcgaaa caacattgaa attctaatgc ctaaacagtt gtagtaattt
     7141 acgaacggct tccatttggc gccgtcccta cgggactctt ccctttctta tttccccgct
     7201 tcccgccact gaagtggcgg gctgaaatct gccgtccctg acgggactgg catcggacga
     7261 gtccggaaaa ccatcgatcg ttcaaatcgg tgattcctgg cccgtagctt cattcctcgt
     7321 tgtaaacggc aggtgcacga caaaatcgtt gatgattgga ttctgtgaat tttgatttgc
     7381 gatgttcgaa tttcggaatt tcccgcctac tgtctcagcc ttgccagccg gacggcgtcc
     7441 tccagcaggc cggggcaatc ggttgaggcg aagccgttga tgccgcgcca gtcgcgatgg
     7501 ccgcggatcg tcatcttgcg tccgtagtac tcctgaatca gttcggccag gtcgacgacc
     7561 gaggagacca ttggctcggg ggggcggcgg ctgacgaaat tgccgagccc gcagatgccg
     7621 atgttgcccg cgttgttgcg gccatcgggg gtcgagccga catgcgtccc cttgcggccg
     7681 acgatgcggc cttcccacac ttcgccgcga ctgtcgacga agaagtgata gccgacgtcg
     7741 gcggcgtcga gccggctatg cgggcgcgta tggtcgcgat agatcgaacg gatcagctcg
     7801 gccggcgggt tgttgctgat cgcgcccgtg tgatgaatcg tgatgaattt ggcggcgccg
     7861 atcggctccc atgtgcggtc gcgttcgggc tgggggggaa acgcgtggcc gccggtccac
     7921 tcctcacgct cgtggaaccg ccgccccagc cgctggcgga ggtattcggc caggctcatc
     7981 gagagggcgg gggttggggt ctcggcctga agggcggcgt cgtcggccag gcgcgtgccg
     8041 gcggggttcg gcggcgcggc cgtcacgatc aggacggtcg ccgcgaagac cgcgaccgcg
     8101 cccgccgcaa agatggccat ccggcgcgaa aacggatggg actgggctcg gctgggtcgg
     8161 tcgggattca tccgagtcag gggcccctcg atcgggatca ggcgggccat tcgcccgcat
     8221 gcagattagc acaagccaac ggcaatgcct acccggcagg cgccgccggg ggggggcctg
     8281 gttgcggctc gtggcggacc aggggttcgc ggtgtgggcg cccgcgaacc cctgcggacg
     8341 tgggggatca aggagaggga gaaacttcag tgggtttgcg ccagccgcgc ggcccggggc
     8401 ggctcggacg gttacctgcc tggtttcctt gcgcgggaat tccccgtgcg ttggccgcgg
     8461 catcggtggc actctcgggg atcggtccgg tatgcgtatg ccaatgatcc ggggcccgac
     8521 cccgtgaccc ttcctcgtgt ttaaggaggt tcctcgcggc aaaaaggttg cactggaatg
     8581 gaactcgaag cacgaagttg cgcctggtga aggcggtctt tcggcagatc ccgcgaaatc
     8641 caacaggtgg cgataccgat ggcgataccg ataccgctgg cgatcgctaa agccgacggt
     8701 actcttcctc gagttcgaac tcgggggagg tgacgatcga ttcgatccgc tggaggcgcc
     8761 ggtcgagcgc ctggaagcgt tggcgcagct tgcccatggc ggcgcccctg gaggcgtgat
     8821 acaggtccca cagttcctct tcctgttcgc tctcgaaagc ggggcggagc ggggcgggcg
     8881 gcaggatgac ccccagcacg atgtagagca gaatcagggg gaagaagccg ctgaagatga
     8941 acaggatcac cgcgaggagc cggacgccgg cgggactgaa atcgaagtgc tcggccaggc
     9001 cgccgcagac gcccagcagc atgcggtcgg tgctgctgcg gaagagcctg ggtcgcccgg
     9061 catacgaacg gtgtttcatt tcagtccctt tcatcgcccg tatggctatc gctctcggga
     9121 tcgagatcgc gatcgctgca tcaggatcgt tgccaggttt tccagccggc gttccatctc
     9181 gtcggcccgg tgcatcaagc tatggctttg ccagggaagc catggtccgt tcctcaccag
     9241 ggttcaaagt ggcggcggcg catccgctcg gccagaactc cttcgaggcg cgccatccgc
     9301 tcctccgcgc ggcggaggct ctcatccatg gcggcgtgga tgtcgtcgcg ccggcgcatc
     9361 gaatggccgt agtggctgcg ccaggcggcc gcggcgcggg ggcggtcgcc gcgccggaag
     9421 agggcgcgca ggatgagccc gccgatcgcg agaagcgcca cgccgagcag gacgagcgtc
     9481 agcccggcct tcacgatgag cagcaccagg ccgccgacca gcttgagcac gaatccgacg
     9541 atgccgaaga cgaagctcag gaggccggcg acgaggccga caagaccgcc caggacgccc
     9601 gcgacgaggc cgccgagcag ccagacgccc agcatgacca gggcgatgat tccgatcagc
     9661 ttgagcattc tctccatcct tgttccttcc atgatgcgcc gaaatttcgt ccgatagtgt
     9721 tgattaggcg ccgattcaga gccgttccgc ggcgccggag cgcccgcgct ccagcagaat
     9781 cgtctccagg ttctcgatgc gccgttccat cttctccatg ccgctcatca gggcgcgcag
     9841 ctcgtcctgg gcggcgcgtt gcgcgttcag cgattcctcg cggcggttgc cgccccggac
     9901 gacgcggaat cccacccaca cgatcagcgc gatcaggagc agcgggccga gggccgcgat
     9961 cgcgaagagc ataacgggca tcaggatggc cgcaagcggg atgatgggtt ccatggatca
    10021 gcgtccttcg tttccgcgaa ttcgcgcgct caagcaattc caggctggcg gcctcatccg
    10081 tccgaccgcc gcttcggttg cggcggggcg tggaccaggc gtgcccgact tcaagattac
    10141 catagcaacg gggccgagcg tgaggatgac agggctgggg ggagctgagg ttcagcgggc
    10201 ggcaccgttt cgatcatgca agacctcaaa gaccaatcaa gccgattcgg gatgcggcgc
    10261 ggccgactgc cccccacggg atcccttgca cgacgcccag gcggcgtttc aagcgttccg
    10321 cccggccgaa cccgaccgga gctgctcctt cagccgctcc agttcctgct cgatctcgtc
    10381 gctgtattcc aggtcggcca gctcgtcgcg cagcgtggga cggcgcgggc gcaggctgtc
    10441 gaccaggtcg gcctcggcct ccagccggtc gatgccgcgc tcgtaggctt cgaacctggc
    10501 gaacgcctcg gtcgtatcga cgcggcggac gtgggcggcg gtacggaagc gcgactcggc
    10561 ggcctggcgg cgctggatga tcgtccgctg cttctcgcgc gcgtcgttga gcttggcttc
    10621 gagctgggcg atgtcgtcct tgaagctggc gacgttctcg cgcgtgcggt ccagttcctc
    10681 ctcgagcgag cggacgcgct gttcgtggcg gcgcttctcg gccagcgccg cgcgggccag
    10741 gtcctcgcgc tgcttctgga gggcgaggcc ggctttggcc tcccagtccg cggcctcgcg
    10801 gcgggcgccc ttgagcgcgc gctcgagctt cttctgctcg gccaggacgc cggcgcagtt
    10861 ggacttgacc tcgatcagcg tgtcctccat ctcctggatc atcagccgga ccattttttc
    10921 ggggtcctcg gcccggtcca ggatcgcgtt gaggttggcg ctgacgatgt cgcggaatcg
    10981 ggtgaagatg cccatggcga tgcttccttg tggttgacgg tctgtgagat cgccggtggc
    11041 gaagcccatg gcgatgcttc ctgttggcgg gtggcgagtg cgagaaccgg gccaaaggct
    11101 ctgatttcag ataagtgatg ttttatcaat ataatacgtg aaatcgggcg cgtaacgatg
    11161 gccgattttg agtggtcttg tagacatatt tttggtgatt cacaccatta atctggctac
    11221 agtcaccaca tggttgtgag cgggacatct cccgatccgc cgcaggcgtt aggcgaatct
    11281 gaggcgtttc tggcgctgca ggagcggatc tcgcaggcgg cccggaccga ccggccggtg
    11341 atcctgatcg gcgagcgcgg cacggggaag gaactggccg cggcgcggct gcacctgctc
    11401 tcggcgcgct gggggcaacc ctacgtcacg ctcaactgcg cgtcgctggc gccgacgctg
    11461 atcgaatcgg agctgttcgg ccacgaggcg ggtgcgttca cgggggccgt gcggctgcgc
    11521 aagggccggt tcgagatcgc cgacggcggc agcctgtttc tggacgaggt cgccaacatc
    11581 ccgccgacgg tgcaggagaa actgctgcgg acggtcgaat acgggcagat ggagcgggtg
    11641 gggagttcgg agacgcggcg agtggacgtg cggctgatcg cggcgaccaa cgtggatctg
    11701 cgcgccgaag cggaggcggg ccggttccgg cgggatctgc tcgaccggct cgcgtttgag
    11761 gtgatcgtgc tgccgccgct gcgtcgccgg cgggaggata tcgtgctgct ggcgcgccac
    11821 ttcgcgcgcc ggatggccgg ccagctcggc ctggagttcc agccggagtt ctcgcccgcg
    11881 gcgcggcagg cgctgctggc gcatccgtgg ccgggcaatg tgcgcgagct gcggaatgtc
    11941 gtcgagcgga tggtctaccg cacgcgcgcc cgcttgattg aggagatcgt cttcgacccg
    12001 ttcgactcgc ccgacatgcc gggtggcggg ccaggcccgg ccggaaacgc gcgcgccgcg
    12061 tcgtcgccgg aggccgcggc cggcctcgcc gagctgaacg ccctcccctt gccc
//
LOCUS       DAOPCO010000101        12026 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5253_length_12026_cov_5.857405, whole genome shotgun sequence.
ACCESSION   DAOPCO010000101 DAOPCO010000000
VERSION     DAOPCO010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 12026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12026
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5253_length_12026_cov_5.857405"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLS90_08605"
     CDS             <1..346
                     /locus_tag="PLS90_08605"
                     /inference="COORDINATES: protein motif:HMM:NF028226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HPK02505.1"
                     /translation="IVVLFILYSSWRVVRENVLLLSDAAQLDPGPIREVVEAVEHVAN
                     SHAIRTHGMPDDVHLDLHIVVDAELTAAQTQRVEMEVRQAVQNRFPQVSEVSIHHQTE
                     QPRTGAPLIRPR"
     gene            complement(354..1511)
                     /locus_tag="PLS90_08610"
     CDS             complement(354..1511)
                     /locus_tag="PLS90_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02506.1"
                     /translation="MHDPRQPASARRRPLGRGRRIAFAAVTLVFMAGVLLGALELFLR
                     AADLRAGMVFHPAPEGYMRLDAEAGYVFAPNYPPHLRRYFDGENQTLLYTNSLGMRDV
                     EPPPPDGRRRILTLGCSVTEGVGLAEPEAPWPRQLERLWREKAGGAEPPVVVMNGAAL
                     GYNTFQQVARARQLQPHVRADVWLMGWLDGQNGMWVRNLFGAQGQFVYRLGFIWDRKY
                     WDCFAGGRSEWTLWLVQHSALVRFILFRAADWSDCPDPAGPYGMDLRAASRDALERFR
                     AATDEAGVEPWIVILPAPADLERWRQAPRGDAAEQVAAICADLGLPCVDATPAIQARL
                     ADATAQSGKGVIGLWALSEYDQQHYNPAGHRMLAEVIDALIPLGPAARRDE"
     gene            complement(1593..1868)
                     /locus_tag="PLS90_08615"
     CDS             complement(1593..1868)
                     /locus_tag="PLS90_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02507.1"
                     /translation="MAKANKPANAPRKSAYELWALLFCFGGAALALLIALGAHAAGGR
                     VGLAFWVFVVTQVAAIGLGLLTWRSPQGRAAAITATVLILLTLMALR"
     gene            2228..2788
                     /locus_tag="PLS90_08620"
     CDS             2228..2788
                     /locus_tag="PLS90_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02508.1"
                     /translation="MSFDCFFFRLADGIDVEELNQFLSSADYEEYLDEGDDSAAAGDE
                     PLLVPARFLDLDLDEEELNELLTRAGEELNKPELNDFLQEALAEAAEFNGMLQIAVGN
                     NDHPRELLRDFAEILKRLEADRIALFLDETSQAYDAANADELAEVADQPTHDFELAYD
                     EDEMDDQDEMDDDEDLDDDSEEDDDR"
     gene            2905..3681
                     /locus_tag="PLS90_08625"
     CDS             2905..3681
                     /locus_tag="PLS90_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02509.1"
                     /translation="MSYDLEFFRLADGVSKAQVNDFLRSDAYMAFCKEQERLEEALAE
                     NPAEAHDMVFTTLPTRFVDTSLDEERLLGLLGRALLQRSIPPAERSPELQAWLERRPG
                     AELPPEHVEDLSLAFEYAGTPGTQPMMFSMRGLNGALTMVAGMLEDLKEERIVVFDPQ
                     LAAIVDGDDARALLTAMGREASAIFNSLLASIDPNAADDEGADYEDADDEEEDEEDWE
                     DDEDDEDWDDDEDWEDDEDWDGDEDEDEDDDEDDDLDDDE"
     gene            4021..5169
                     /locus_tag="PLS90_08630"
     CDS             4021..5169
                     /locus_tag="PLS90_08630"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK02510.1"
                     /translation="MAEPPAPPPDPSPPGVQVRPRRRPILTTALVVLCTLIFLAFSHL
                     NGWRGIVSLELLERFWVLRAERIWNGEFRALLAAPFVHVDPFHLAVNMVFLWLIGNRV
                     ERLLGHQTMFAVVFFGAIISSGCELALGEWAPMVGCSGVICALFGLMVALRRRAPMFH
                     HFLRPPMLHVILGLLALSALLDLVGLLPVANVSHLSGLIFGWAVGHLFFQGRCRWPAA
                     AALAALVALCVMGATWLPFSSHWVAWRYLKAQVEGQSARADAVLEHSPFRDDPETLNH
                     IAWRLATSYDKRRRDGARAVELARRACEASNWQWPEIIDTLAAAYAESGQWGEAAATS
                     ERAVALLEQLLADETGAVAPQRKNLLRLMAPSLRENLESIRKGEKIRE"
     gene            complement(5206..5595)
                     /locus_tag="PLS90_08635"
     CDS             complement(5206..5595)
                     /locus_tag="PLS90_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK02511.1"
                     /translation="MSTMQPSITRLAVDYRPHPNICLREMLIQGDQLTVQLDGQILFA
                     SAGPLRRLLQEQAEDYAPRRLVLDCRRVTFVDSEGLAMLVGLDRHCRGNAIELWLKNP
                     GEHLARLLKMTRLDQILAVAPQTTGDD"
     gene            complement(5595..9374)
                     /gene="dnaK"
                     /locus_tag="PLS90_08640"
     CDS             complement(5595..9374)
                     /gene="dnaK"
                     /locus_tag="PLS90_08640"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK02512.1"
                     /translation="MDGQGHVIGIDLGTTNSVVAVIIGGEPEVIPNAEGQSKTPSVIA
                     LLDDGQIIVGEIARRQAATQPQRTISSAKRLIGRFYNEVVEEGEQYPFELDEAEDGRL
                     VIPVGDRAFLPEELSALVLEKLKLAAEHYLGESVERAIVTVPAYFDDMQRQATLEAAR
                     LAGLEVLRLINEPTAAAMAYGLGREDQAIVAIYDFGGGTFDFSVLDIENKTFEVLVST
                     GNSRLGGDDLDDLLVDLLAERFLAEHQIDLRDDLLTLRRLKDAAEAAKCELSTAVETL
                     VSLPFISYRDGQPLHLEIAVTRDEFEDLIEPLVRETIDCCRKGLRDAGLKSESIQRVI
                     LVGGSTRIPLVQESVEEFFGLTPFKGVNPDEVVAIGAATQGAVLNGELDEVVLLDVTP
                     HNLGIEIRGNRVSTVIEKNSTIPLKVEKTFTTTEPEQTFVNIHVLQGEGEKASDCRSL
                     GRFTLSGIEPAAPGVPRIRVTFLVNADGIIEITASDSQSGAERSLKINHAYLNGEERR
                     SREKQSVRRQRRRRSQRVGAPAAAGSTGAEPVEAASTGGARSLAGARRKAAMAAAHAQ
                     SQAQSEHGEFAEVTTGGSSSWREGRQHTKPALEPAPAAEIAAAPAPPPAAAARGGKPA
                     APATPAAARKSERQEVVAAARPSTPAREAQPADATPLAVRHSARAAELAETCVRGEQA
                     PDGTGAAGGAPMPGSNTERGCLPHINTPVPPELSQTVDLLTRNSEDELARKTYEGARD
                     LLVHWVSEHAEDASLQLLLARYHLIMNRAEDARQILQQVHATAPDLREPVRVLYGALC
                     RRFPNYLAARHDRARLALDADDLLTAMGELEFLLAREEQNPEIADLLGGVYERIIECN
                     PDPHVQFKLVKLHLRRQKLDAAIGLLQQLVHNREYRQRASRVLGLCFWQKGMRYLAWQ
                     KLRELPVDEEMKDILYRLSADMELNDELLHARYALERIYEADIQYRDVAERMEKLAYR
                     VKLQEDERYGGPKGGGEKEEGAPQPFHAGGEFVRRFELLEELNRGSMGIVFRARDKML
                     EEVVAIKVLNDFLCADPEAIERFKQEARAARKLTHQNIVRIHDMFEMDGHKIISMEYI
                     DGEDLKALLKRNMTFSEDITLNFLMQICEGLAYAHRLNIVHRDVKPANIMITDRNQVK
                     ITDFGIAKILTANQTIAGTTVMGTPLYMAPEQIEGGQIDHRCDIYALGIMLYELVTGR
                     PPFCEGNIEYQHLNSPAPEIKAGISDRLKGIIMKCIEKSPEKRFQTVEEILARII"
     gene            9762..11393
                     /gene="murJ"
                     /locus_tag="PLS90_08645"
     CDS             9762..11393
                     /gene="murJ"
                     /locus_tag="PLS90_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPK02513.1"
                     /translation="MSEKRTVLKAAGVVGGITLLSRFTGLIRDVLITALFGARGIADT
                     FFVAFEIPNLLRRVLGEGALSSFLVPLFSQRRQEEGDAGGWRFFNLTLNFMLVVSLAL
                     TVLGILFSREVYLVAGGLGQLLRGGDAGQTGRMVDLGGRFTAIMFPFLIGLSMASLMM
                     GACHALRRFVAPSLGSVMLNVALIAAGGAALALNLEQAPAAIWLCWAVLVGSLVRVLI
                     MLPTLHAHGWRWRPVLNLRDPELYRLLRMLATGTLSMSISQINIVVAGFFALYLGEGI
                     KTYLMTANRLVQLPMALTATAVATAMLPQLTQYLLEGRRRELGDLMGFTKRLEIVLML
                     PAIAGLIVFGQPIMELLFERRLWSAADTRAATLALLCYAPSLLPLGGARLLTSLFYAR
                     KDVVTPLKAGALSMLVNVILNAVFALSLPLAQAGLALAFTVGTYVNYALLAWYARRDF
                     GEAMTEQKQVGATLARSTAAALLACGAGALAYYPLRRWLPIPLDSALTRGALLLPVIA
                     LVAGLYFVLAHALRVPDADRAKSILTRRLKLNRKP"
     gene            11846..11922
                     /locus_tag="PLS90_08650"
     tRNA            11846..11922
                     /locus_tag="PLS90_08650"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11880..11882,aa:Pro,seq:tgg)
BASE COUNT         1837 a         4340 c         3720 g         2129 t
ORIGIN      
        1 gattgtcgtc cttttcattc tctattcgag ctggcgcgtg gtgcgtgaaa acgtcctgct
       61 cctctccgac gccgcgcagc tcgatccggg gccgatccgc gaggtcgtcg aggcagtcga
      121 gcatgtggcc aacagccatg cgatccgcac ccacgggatg cccgacgatg tccatctgga
      181 cctgcacatc gttgtggatg ccgagctgac ggcggcgcag acccagcgcg tcgaaatgga
      241 agtgcgccag gccgtgcaga accgattccc gcaggtcagc gaggtctcga ttcaccacca
      301 aaccgagcaa ccgcgcaccg gcgccccgct gatccgtccg cggtgatgcc tcgtcattcg
      361 tcgcgccgcg cggcgggccc cagcggaatc agcgcgtcga tgacctcggc cagcatccgg
      421 tgacccgccg gattgtagtg ctgctggtcg tactccgaca gggcccacag cccgatcacg
      481 cccttcccgc tctgcgccgt cgcgtccgcc aggcgcgcct gaatcgccgg cgtcgcgtcg
      541 acgcagggca ggcccaggtc ggcgcagatc gccgccacct gctcggccgc gtcgccgcgc
      601 ggcgcctgcc gccagcgttc aagatcggcc ggcgccggga ggatgacgat ccagggctcg
      661 acgcccgctt cgtcggtcgc cgcacggaac cgctccagcg cgtcacggct ggccgcgcgc
      721 agatccatcc cgtacggacc cgccggatcg gggcagtcgc tccagtcggc cgcgcggaac
      781 aaaatgaagc gcacaagggc gctgtgctgg acgagccaca acgtccattc gctccttccg
      841 ccggcaaagc agtcccagta tttgcggtcc cagatgaatc cgaggcgata gacgaactgc
      901 ccttgcgcgc cgaacaggtt gcggacccac atgccgttct ggccgtcaag ccagcccatc
      961 agccagacgt cggcccgcac gtggggttga agctgacgcg cgcgcgcgac ctgctgaaac
     1021 gtgttgtagc cgagcgcggc gccgttcatg acgacgacgg gcggctcggc gccgccggcc
     1081 ttctcccgcc acaaccgttc cagctgacgc ggccagggag cctccggctc ggccaggccc
     1141 accccttcgg tgacgctgca gccgagggtc aggatgcgcc ggcggccatc gggcggcggc
     1201 ggttccacgt cgcgcatgcc gagcgaattg gtgtagagca gggtctggtt ctcgccgtcg
     1261 aagtaacggc gcaggtgcgg cgggtagttt ggggcaaaca catagccggc ctccgcatcg
     1321 aggcgcatgt agccttcggg cgcggggtga aaaaccatgc cggcgcgcag atcggccgcg
     1381 cgcagaaaca gctcgagcgc gccgagcaac acccccgcca tgaagacgag cgtgacggcg
     1441 gcaaacgcga tccgccgccc ccggccgagc ggccgccggc gcgcggaagc gggttgtcgc
     1501 gggtcatgca tggtgagacg aatcgcccgg tccggcttgg aactgcttcc cccgcagctg
     1561 gctgaccgcc gaattcggcc cacgggcccg gctcaacgca gcgccatcag cgtcagcaga
     1621 atcagcacgg ttgcggtgat cgcggcggcg cggccctggg gactgcgcca ggtcagcagg
     1681 cccaggccga tcgctgcgac ttgcgtgact acgaaaaccc agaaggccag cccgacgcgg
     1741 ccccccgcgg cgtgagcgcc cagcgcgatc agcagcgcca gggcggcgcc gccaaaacag
     1801 aacagcaacg cccagagctc gtaagccgac ttgcgcggag cgtttgccgg tttgttcgcc
     1861 ttcgccatga atctgataac ctccctgggc gaaaggttta gcggacacgt atttcagtca
     1921 ttgtggtcgg ctgtgcccgc cggcgcaacc cgaaataagg aacatggaat cgaataagcc
     1981 cccgccctca ctctggatcg ttcctcttct ctcgtctctc gtccctcgcc ctcgtttcgt
     2041 atcccttatg cccttacgtc cccgtcctct ctgtcctttc cgctattctc cggcgttgag
     2101 cttcccgctt gcctttttcc agcgcttcgt gaataactgc gccgtccgcg gtggcatcga
     2161 ttcttttctg gcgcacccgc gaccggattg cggcgcggcg ccgccgaact ccccggagaa
     2221 tcgctcgatg agcttcgact gcttcttctt ccgcctggcc gatggcatcg acgtcgagga
     2281 gctgaaccaa ttcctcagct ccgccgacta tgaggaatac ctcgacgagg gcgacgactc
     2341 ggcggccgcc ggcgacgaac cgctcctcgt ccccgcccgc tttctcgacc tcgatctgga
     2401 cgaagaggaa ctgaacgagc tgctcacccg ggccggcgag gaattgaaca agccggaatt
     2461 gaacgacttt ctccaggagg cgctcgccga ggcggccgaa ttcaacggca tgcttcagat
     2521 cgcggtcggc aacaacgacc atccgcgcga gctgctgcgc gatttcgcgg aaatcctcaa
     2581 gcgcctcgag gccgaccgca tcgcactctt cctggacgaa accagccagg cctacgacgc
     2641 cgccaatgcc gacgaactgg ccgaagtggc cgatcagccg acccatgatt tcgaactcgc
     2701 ctacgatgag gatgagatgg acgatcagga cgagatggac gatgatgagg acctcgacga
     2761 cgacagtgag gaggacgacg accgctagaa acgtcgcggc cgaacgggcc atcaacagcc
     2821 ggcgagggcg tccgccaggg cttggtgcgg ccgccacgcc acttgtcggc ggcgaccctg
     2881 actttcgcac cgggagggac ggcaatgagc tacgatctcg aattcttccg cctggccgac
     2941 ggagtgtcca aggcgcaggt caatgatttc ctccggtcgg atgcttacat ggcgttctgt
     3001 aaggaacagg agcgcctgga agaggcgctg gcggaaaatc cggcggaggc ccacgatatg
     3061 gttttcacca cgctccccac gcgctttgtc gacacctcgc tcgacgagga gcgcctgctt
     3121 ggtttgctcg gccgcgccct cctccagcgt tccatccccc ccgccgagcg ttcccctgaa
     3181 cttcaggcct ggctggagcg ccggcccggc gccgagcttc ccccggagca cgtcgaagac
     3241 ctcagcctgg ctttcgaata cgccggcaca cccggaaccc aaccgatgat gttcagcatg
     3301 cgcggattga atggggccct gacgatggtg gccggcatgc tcgaggacct caaggaggaa
     3361 cggatcgtcg tgttcgaccc ccagcttgcg gccatcgtcg acggcgacga cgcgcgcgcg
     3421 ttgctcaccg cgatgggccg cgaagccagc gcgattttca acagcctgct cgcttcgatc
     3481 gatcccaacg ccgccgacga tgagggcgcg gattacgaag acgcggacga cgaggaggaa
     3541 gacgaggagg attgggagga tgacgaggac gacgaggatt gggatgacga cgaagactgg
     3601 gaggatgacg aagactggga cggcgatgaa gatgaagatg aagacgacga tgaggatgac
     3661 gacctcgacg acgacgagta accgcccgtc cgactagatt tccgtccctc gtcctcgctc
     3721 aaaaatccgt ctctcgttct cgtcctcgtc ttcgtgatcg ctcccaacgg cccaccccaa
     3781 gattcggctc ccggctctcg tccctcgtcc tcggctctcg aaaaccttcg tctccgtcga
     3841 tctcccaaag caacgatcgc cgccccacgc acccttcaca ccatccgtct ctcccaagcc
     3901 ctcccacggc cagactggcg tcggcacgct tgttccgtta aattggggcg ggctgggccg
     3961 cctccacgga ttgcttccac cggcccgcct ccccgaccgc ccccaggttg tctccgccgc
     4021 atggccgaac cgcccgctcc gccccccgac ccgtcgccgc cgggcgtgca ggttcgcccg
     4081 cgccggcggc ccatcctgac caccgccctc gtcgtcctct gcacgctcat ttttctcgcc
     4141 ttctcccacc tcaacggctg gcgcggcatc gtctcgctcg aactgctcga gcgcttctgg
     4201 gtgctgcgcg ccgagcgcat ctggaacggc gagttccgcg ccctgctggc cgcgccgttc
     4261 gtccacgtcg acccgttcca tctggccgtc aacatggtct ttctctggct catcggcaac
     4321 cgcgtcgagc gcctgctggg ccatcagacg atgttcgccg tcgtcttctt cggcgcgatc
     4381 atcagctccg gctgcgagct ggcgctgggc gagtgggccc cgatggtggg ctgctccggc
     4441 gtcatctgcg cgttgttcgg cctgatggtc gcgctgcgcc gccgcgcgcc gatgttccat
     4501 catttcctcc gcccgcccat gctgcatgtc atcctcggcc tgctggccct ctccgcgctg
     4561 ctcgacctgg tcgggctcct cccggtggcc aatgtctccc acctgagcgg cctgatcttc
     4621 ggctgggccg tcgggcatct cttctttcag gggcgctgcc gctggcccgc cgccgccgcg
     4681 ctcgccgccc tggtggccct ctgcgtgatg ggcgcgacct ggctgccgtt cagcagccac
     4741 tgggtcgcct ggcgctatct gaaggcgcag gtcgagggac aatcggcgcg ggccgacgcc
     4801 gtgctggagc attccccgtt ccgcgacgat cccgagacgc tcaatcacat cgcctggcgg
     4861 ctggccacgt cgtatgacaa gcgacggcgc gacggcgccc gcgcggtcga gctcgcccgg
     4921 cgcgcctgcg aagcctcgaa ctggcaatgg ccggagatta tcgatacgct cgccgccgcc
     4981 tatgccgaga gcggccagtg gggcgaggcc gcggcgacct ccgagcgggc ggtcgccctg
     5041 ctggagcaac tgctggcgga tgagaccggc gcggtcgcgc cgcaacgcaa aaacctgctg
     5101 cggctgatgg cgccgtcgtt gcgcgaaaat ctggagtcga ttcgtaaggg tgaaaaaatc
     5161 cgtgaatagc cgacccgccg cggcgcgggc cgccgcgcac gccggctaat cgtcgccggt
     5221 cgtctggggg gccacggcca gaatctggtc gagccgcgtc atcttgagca accgcgccag
     5281 gtgctcgccg ggattcttca gccacagctc gatcgcgttg ccgcggcaat ggcgatccag
     5341 gccgacaagc atcgccagcc cctcgctgtc gacgaacgtc acccgccggc agtccagcac
     5401 cagccgccgc ggcgcgtaat cctccgcctg ttcctgcagc agccggcgca gcggccccgc
     5461 cgaggcaaac aggatctgcc cgtcgagctg cacggtcaac tgatccccct gaatcagcat
     5521 ctcgcgcagg cagatgttgg ggtgcggccg gtaatcgacc gccagccgcg ttatgctggg
     5581 ttgcatcgtg ctcatcagat gatccgcgcc aggatctcct caaccgtctg gaaccgcttc
     5641 tcggggctct tttcgatgca tttcatgatg atccccttga gccggtcgct gatgcccgcc
     5701 ttgatctccg gcgccgggga attcaggtgc tggtactcga tgttcccctc gcagaacggc
     5761 ggccggcccg tgaccagctc atacagcatg atccccagcg cgtaaatgtc gcagcggtga
     5821 tcgatctggc ccccctcgat ctgctcgggc gccatgtaga gcggcgtccc catcacggtc
     5881 gtcccggcga tcgtctgatt cgccgtcaga atcttcgcga tcccgaaatc ggtgatcttc
     5941 acctgattgc ggtcggtgat catgatgttg gccggcttga cgtcgcggtg cacgatgttg
     6001 agccggtgcg cataggccag cccctcgcag atctgcatca ggaaattcag cgtgatgtcc
     6061 tcgctgaacg tcatgttgcg cttgagcagc gccttcagat cctcgccgtc gatgtactcc
     6121 atcgagatga tcttgtggcc gtccatctcg aacatgtcgt ggatccggac gatgttctga
     6181 tgcgtcagct tgcgcgcggc gcgggcttcc tgcttgaacc gctcgatcgc ctcggggtcg
     6241 gcgcagagga aatcgttgag gaccttgatc gcaacgacct cctccagcat cttgtcgcgc
     6301 gcgcggaaga cgatccccat cgacccgcgg ttgagctcct cgagcagttc gaagcggcgg
     6361 acgaattccc ccccggcgtg aaacggctgc ggcgctccct cctccttctc gccgcccccc
     6421 ttcgggccgc cgtagcgctc gtcttcctgc aacttgacgc gatacgccag cttctccatg
     6481 cgctcggcca cgtcgcgata ctggatgtcg gcctcgtaga tccgttccag cgcatagcgc
     6541 gcatgcagca gctcgtcgtt cagttccatg tcggccgaga gccggtaaag gatgtccttc
     6601 atctcctcgt cgaccggcag ctcgcgcaac ttctgccacg ccagatagcg catccccttc
     6661 tgccagaagc agaggcccag cacgcggctc gcgcgctggc gatactcgcg attgtgcacc
     6721 agttgctgca gcaggccgat cgccgcgtcg agcttctgcc gccgcaggtg cagcttgacc
     6781 agcttgaact gcacgtgcgg atccgggttg cactcgatga tccgctcgta gacgcccccc
     6841 agcaggtcgg cgatctcggg gttctgctcc tcgcgggcga gcaggaattc cagttcgccc
     6901 atcgccgtca gcagatcgtc ggcgtccagc gccaggcggg cgcgatcgtg ccgcgccgcc
     6961 aggtaattgg gaaaccgccg gcagagcgcg ccgtagagca cgcgcaccgg ttcgcgcaag
     7021 tccggcgccg tggcgtggac ctgctgcagg atctgccgcg catcctccgc gcggttcatg
     7081 atcaggtggt agcgcgccag cagcagttgc agcgacgcat cctcggcgtg ctcgctgacc
     7141 cagtggacga gcaggtcgcg cgccccctcg taggtcttgc gcgccagttc atcctcgctg
     7201 ttgcgcgtca gcaggtcgac cgtctggctc agttcgggcg gcacgggcgt attgatatgc
     7261 ggcaggcagc cgcgctcggt attgctgccg ggcattggcg cgccgccggc cgcgccggtg
     7321 ccgtcgggcg cctgctcgcc gcggacacag gtctcggcca gctcggccgc gcgcgccgaa
     7381 tgacgcaccg ccagcggcgt cgcatcggcg ggttgggctt cgcgcgccgg tgtggagggc
     7441 cgcgccgccg cgacgacttc ctggcgctcc gacttcctgg ccgccgccgg tgtcgccggc
     7501 gcggccggtt tgcccccgcg cgccgcggcc gccggcggcg gcgcgggggc cgccgcgatc
     7561 tccgccgccg gcgccggttc cagcgccggc tttgtgtgct gtcgcccttc gcgccagctc
     7621 gaactgccgc ccgtcgttac ttcggcgaat tccccgtgct cggactgcgc ctggctttgc
     7681 gcgtgcgccg ccgccatcgc ggccttgcgc cgcgcgccgg ccagcgagcg cgctcccccc
     7741 gtgctggccg cctcgaccgg ctcggccccg gtggaccccg ccgccgcggg cgcgccgacc
     7801 cgctgcgacc gccgtcgccg ctgccgccgc accgattgct tctcccggct gcgccgctct
     7861 tcgccgttca aatacgcatg gttgatcttc agcgagcgtt ccgcgccgct ctgcgaatcg
     7921 ctggccgtga tctcgatgat cccgtcggcg ttcaccagaa acgtcacccg aatccgcggc
     7981 acccccggcg ccgccggttc gatccccgac agcgtgaacc gccccagact gcggcagtcc
     8041 gacgccttct ccccctcgcc ctgcaacacg tgaatattga cgaaggtctg ctccggctcg
     8101 gtcgtcgtaa acgttttctc gaccttcagc ggaatcgtgc tgttcttctc aatcaccgtg
     8161 ctgacccgat tgccgcgaat ctcgatgccc agattgtgcg gcgtcacgtc cagcagcacg
     8221 acctcgtcca gttcgccgtt gagcacggcg ccctgcgtcg ctgccccgat cgccaccacc
     8281 tcgtcggggt tgaccccctt gaacggcgtc aggccgaaga actcctccac gctctcctgc
     8341 accagcggaa tccgcgtact cccgccgacc aggatcaccc gctggatcga ttccgatttc
     8401 agccccgcgt cgcgcagccc cttgcggcag cagtcgatcg tctcgcgcac cagcggctcg
     8461 atcagatcct cgaactcgtc gcgcgtgacg gcgatctcca ggtgcagcgg ctgcccgtcg
     8521 cggtacgaaa tgaacggcag gctgacgagc gtttccacgg ccgtcgacag ctcgcacttg
     8581 gccgcctcgg cggcgtcctt caaccggcgc agcgtcagca ggtcgtcgcg cagatcgatc
     8641 tggtgctccg ccaggaaccg ttcggccagc aggtccacca gcaggtcgtc caggtcatcg
     8701 ccgcccagcc ggctgttgcc ggtcgagacg agcacctcaa acgtcttgtt ttcgatatcc
     8761 aggacgctga aatcgaacgt tccgccgccg aagtcgtaga tcgcgacgat cgcctggtcc
     8821 tcgcgcccca ggccgtaggc catcgcggcg gcggtcggct cgttgatcag ccgcagcacc
     8881 tccagcccgg ccagccgcgc ggcttccagc gtcgcctggc gctgcatgtc gtcgaaatac
     8941 gccggcaccg tcacgatcgc ccgctcgacc gattcgccca gataatgctc ggccgccagc
     9001 ttgagcttct ccagcaccag ggccgacagc tcctcgggca ggaacgcgcg gtcccccacc
     9061 gggatcacca gccggccgtc ctcggcctcg tccagctcga acggatactg ctcgccctcc
     9121 tcgaccacct cgttgtagaa gcggccgatc aggcgcttgg cggaggaaat cgtgcgctgg
     9181 ggctgcgtcg ccgcctggcg ccgcgcgatt tcccccacga tgatctgccc gtcgtcgagc
     9241 agcgcgatca ccgacggggt cttgctttgc ccctcggcgt tgggaatgac ttcgggttcg
     9301 ccgccaatga taacggcgac gacggaattt gtcgttccaa gatcgattcc gatgacgtga
     9361 ccttgtccgt ccatggagcg gtctggtggc ctcctggggg tgctgtcgag gtcagcgcaa
     9421 tcgaaccgcg gcgagggggc aaaccagctc gcgtccgaag gcctgtccaa aaccatgcgt
     9481 gatcgccccg atgccggcgc catcggggat cttcgattcc tgtatcggca gccggggccc
     9541 ggaagtgaac ccctattccg gaaagcgacg gtccccggcg cgacttccct cgctttcgct
     9601 cggcgcctct gctaatcact gaggggcgcc cggcggggcc gggcacaggc ccccccgtcg
     9661 cctgcaaggc tgccgtcgca tggagggccg gcggtttttt acttctcaag ccgattccga
     9721 tcccgattcc gatcccgaga gcggtttctc gcccgacccc gatgagtgaa aaacggacag
     9781 tcctcaaggc cgccggggtc gtcgggggga ttaccctcct gtcgcgcttc accggcctga
     9841 tccgcgacgt cctgatcacg gcgctgttcg gcgcccgcgg catcgccgac accttctttg
     9901 tcgccttcga aatccccaac ttgctgcgcc gcgtcctcgg cgagggcgcc ctttcctcct
     9961 tccttgtccc gctcttttcg cagcgccgcc aggaggaggg cgacgccggc ggctggcgct
    10021 tcttcaacct taccctcaac ttcatgctcg tcgtctcgct cgcgctgacc gtcctgggca
    10081 tcctcttctc gcgcgaggtc tacctcgtcg ccggcggcct gggccaactg cttcgcggcg
    10141 gcgacgccgg ccagaccggc cggatggtcg acctgggcgg ccgcttcacc gccatcatgt
    10201 tccccttcct gatcggcctg agcatggcct cgctgatgat gggcgcctgc catgccctgc
    10261 gccgcttcgt cgccccctcg ctcggctcgg tcatgctcaa cgtcgccctc atcgcggccg
    10321 gcggcgcggc cctcgccctc aacctggagc aggcccccgc cgccatctgg ctctgctggg
    10381 ccgtcctcgt cggctcgctg gtccgcgtcc tgatcatgct tccgacgctc catgcccacg
    10441 gctggcgctg gcgacccgtc ctcaacctgc gcgatccgga gctctaccgt ctgctgcgca
    10501 tgctggccac cggcaccctc tcgatgtcga tcagccagat caacatcgtc gtcgccgggt
    10561 tcttcgcgct ctatctgggc gaggggatca agacctacct gatgaccgcc aaccggctcg
    10621 tgcagctgcc gatggcgctg accgccaccg ccgtcgccac cgccatgctc ccccaactga
    10681 cgcagtacct gctcgagggg cgccgccgcg aactgggcga tctgatgggc ttcaccaagc
    10741 ggctggaaat tgtcctgatg ctgccggcca tcgccggcct catcgtcttc ggccagccga
    10801 tcatggaact gctcttcgaa cggcggctgt ggagcgccgc cgacacccgc gcggcgacgc
    10861 tggcgctgct ctgctacgcc cccagcctgt tgccgctcgg gggcgcccgg ctgctgactt
    10921 cgctgttcta tgcccgcaag gacgtcgtca cgccgctcaa ggcgggcgcc ctctcgatgc
    10981 tcgtcaacgt gatcctgaat gccgtcttcg cgctctcgct gccgctggcg caggccggcc
    11041 tggcgctggc cttcaccgtt gggacgtatg tgaactatgc cctgctggcg tggtatgcgc
    11101 gccgcgactt cggcgaggcg atgactgaac agaagcaggt tggcgcgacg ctcgcccgct
    11161 cgacggccgc cgcgctgctg gcctgcggcg cgggcgcgct ggcctactat ccgctgcgcc
    11221 gctggctgcc gatcccgctg gatagcgccc tcacccgcgg cgccctcttg ttgccggtca
    11281 tcgcgctcgt cgccgggctc tatttcgtcc tggcgcacgc actgcgcgtc cccgacgccg
    11341 accgcgccaa gtcaatcctg acccgccggc tcaaactcaa ccgcaaaccc tgaccccttg
    11401 gcggaacgat accaaattca atgcagacgg ttccgaccca gtggccggct gagtccgcgc
    11461 agcgggcgat tcggaataaa gcgcagggtg acggccgcgg gccgggaccc tgcgtcgccg
    11521 cccccaacga gcgaagcccc tggcaggggc ggccggagag gcttgcgccg tccggctgcc
    11581 aatgggatgc cccgcagctc gagaaggcgc cttaagagcc cgcgccggcg ggcttcttcc
    11641 aggccatcgg ccctctcatt agcaccgggc ctcggcctgg tgacccgttg cgataaattt
    11701 tcaaaactgt ttcaacggtt ttcttcacaa tccggacaaa ccgatgtgaa cttcatccct
    11761 cggcatcagg ctgacacttg ccgcccgcgc aaattcaatt ccctttgatt cacaaccgcg
    11821 cgctgataga ctggcggaaa cgcgtcgggg tgtggcgcag ttggctagcg cacctgcttt
    11881 gggagcaggg ggtcgtcagt tcgagtctga ccaccccgac cattgaaaat aaaggatttg
    11941 cccgaacctc aaagcctcag aaaattgctc gtagtagcaa ccgtggtagc aaccccctgc
    12001 cctcgcccgc cggatcggac aggacc
//
LOCUS       DAOPCO010000102        11916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5315_length_11916_cov_5.144676, whole genome shotgun sequence.
ACCESSION   DAOPCO010000102 DAOPCO010000000
VERSION     DAOPCO010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11916
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_11916_cov_5.144676"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PLS90_08655"
     CDS             <1..762
                     /locus_tag="PLS90_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK02514.1"
                     /translation="RGDCLLHKEDARVSECFVHAFENEGIKVHLEVEVSEVQYDGAAF
                     HLKLVGGTRLEGDALLVAVGRRPNSAALQCARSGIELTDEGFIQVDERLRTTCKGVFA
                     AGEVAGQPPFTHAAWEDHLRILDTLEGGKRTRADRALGYCTFTEPQMGRAGLSMKQAQ
                     AAGYRARCVGKPIREIARAAEWCHHEGFFEMVIDEESDRILGATLVGHEAGELVHLFL
                     GLIECKATWRVLADMVAIHPTYGEGLRSMARQLAD"
     gene            875..1381
                     /locus_tag="PLS90_08660"
     CDS             875..1381
                     /locus_tag="PLS90_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009504061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPK02515.1"
                     /translation="MDVGQLGEQLVQWLNFESRPLDVILRLSMAALCGGIIGWDRERE
                     DKPAGLRTHMMVALGAAAFMVLTVEVSRQTTGFAYDPVRTVAGIAGGLGFLGAGTIIQ
                     SRHRVEGVTTAAGIWVVGALGTICGAGLYGVALLTTILAFIILTVVQFFKLRVLHLRA
                     QPPAHKPD"
     gene            complement(1407..2054)
                     /gene="eda"
                     /locus_tag="PLS90_08665"
     CDS             complement(1407..2054)
                     /gene="eda"
                     /locus_tag="PLS90_08665"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPK02516.1"
                     /translation="MQNQALEQIERTGLVPVIAMEDPELAVPLARALAEGEIPVIELT
                     FRTSAAADAIARIRRECPDVLAGAGTVRTPDQVRQAHQAGARFIVAPGFNPDVAQACA
                     ALQLPFVPGVVTPTEIDRALLAGCQVLKFFPAEPSGGIEMLKALAAPFRHTGVKFLPT
                     GGIREDNFAAYLALDIVTAVGGTWIAPAGAIARQEWASITKLCRQAREIARTARG"
     gene            complement(2165..2701)
                     /locus_tag="PLS90_08670"
     CDS             complement(2165..2701)
                     /locus_tag="PLS90_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02517.1"
                     /translation="MLARWPPRLFPLPPFGGGEKVRYIDHERPDPSVAPDGYAPVPAD
                     LWRDYENLRPPPVQPGRVMHRFSTIGEQQGDYDRRSPWYSYPIALAGFWALMLLVGLA
                     VAARGLGERGFHLDGDETWLLILGGIALGTLPASIASLLYLGEARRTEREMLARRRRE
                     KEAAREQARKQLEHDQGW"
     gene            2912..4219
                     /locus_tag="PLS90_08675"
     CDS             2912..4219
                     /locus_tag="PLS90_08675"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HPK02518.1"
                     /translation="MLRYDLAYALALPALMPYLAWRRLARGKYAESAGGMLGRRLPPP
                     AEARRFADGSLWIHAVSVGEVQAARVVADGLQALAPRLPLVISTITETGQAAARRLFP
                     DAVHTYYPVDFSVNVERFQRVFNPRLFVLLESDLWPNFLAHASRRGTRCVLLNADISD
                     RSFPRYLRFRRYLGPALGALDAVCAQTEVDAERFTALGVAPARIRVTGNCKLDLPAQV
                     LDERERRELCARFGLNPERRWIVAGSTHEGEEALILRGFAELRREMPGLGLLIAPRHP
                     ERFAAVEQLARDAAPAGEGWRVGAASRPEPASDPDVVVLDAMGVLARSYGLGAVAIVA
                     GSFCPTGGHNLLEAAAHGVPVVYGPDMHSQREIDRLFKSMCSGLQVRPDELHATLRRL
                     LTDEAFRAAEGRKALQVLERNQGSARRTTEAAREILLATGRWP"
     gene            complement(4280..5365)
                     /locus_tag="PLS90_08680"
     CDS             complement(4280..5365)
                     /locus_tag="PLS90_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02519.1"
                     /translation="MSSQRAQRVVWLAGTRGEILLLGPSFKALCRPPAEGGNPRAVGW
                     LACTGEHGTAAYQTLDALALAPDETGALCHPADDPAVRLRLMLERTESFVHLRRASHV
                     VFAGYGPTAAAAALCCHGRGCRGLWLRPADRLGIIGRLDWEAGLARVIAALAPRVAIL
                     DVPAMPDAVAEGLAAAPPSSPLEAEIPGLRDAAPLALVAIYRREWGVTHGNAASRLIH
                     AAGRWAEAAPERDWVISSTLNMRLEGPFRALERRPANLLRVPPLPYPLWRELLGRAEL
                     VLTDSPLIADEALQRAIPVLTLGERNPDRADHAITPDELNSPALRERLDRARARPVPA
                     NVPPPIPTPHAWITDRIVQWLAREEEP"
     gene            complement(5362..6555)
                     /locus_tag="PLS90_08685"
     CDS             complement(5362..6555)
                     /locus_tag="PLS90_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK02520.1"
                     /translation="MSPTPPGRSRWLEPDKLTESLAALVQINSVNPGFGGPQGGERQV
                     LERAAGILASAGLTPRLVETQPGRPLLRTRLRGRTPGPPLVFETHVDTVAVTGMKIEP
                     WGGRVDRGRLWGRGSTDAKGQAVAMMHALAAWAEADAPPPHDVELVLVHDEEFSFGGA
                     RALLADGLEAVGIVIGEPTSLRIVTAHKGSVRLWLGVAGKSAHGARPHLGINAISAAS
                     ALVRVIDEQYVPELARRSAPLVGSPTINVGQIVGGVQPNLVPPSCRMHVDRRTIPGET
                     TDDLRRELEGLFAAAKQLFPQFEGCIEEVTLDARAVQAPTDSALVRAAQAVTGAAGRP
                     TAPIGVDYATDACELGHAGIPFIILGPGSIDQAHTEDEFIEIAELEAGARVYAGLMGR
                     DLSAP"
     gene            complement(6614..7264)
                     /locus_tag="PLS90_08690"
     CDS             complement(6614..7264)
                     /locus_tag="PLS90_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPK02521.1"
                     /translation="MKCGEPATHKFTRIEKGQIFDVYLCDRHAAETSQYIPKPEIPLS
                     EILEGLLKQEVKAQSGSTAPAGVVCSQCGLRFEAYKRNLMLGCDACYESFEEYLVPDL
                     RRFHGETRHIGRKPGGGRQVPDQQDAPARHRKSAGSLELSVPPIPQAPDKPVQKKQAD
                     PAKAEREHLIRKLRREMQEAIASEDFARAAECRDQIRALAGDMQQAGSDAPAEEKE"
     gene            complement(7612..8328)
                     /gene="ric"
                     /locus_tag="PLS90_08695"
     CDS             complement(7612..8328)
                     /gene="ric"
                     /locus_tag="PLS90_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008670957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HPK02522.1"
                     /translation="MTATLNPEMTVGAFVAERPARSRVFEKLGIDYCCGGKKPLAEVC
                     ARRNLRVEDVLQLIEAADAGPEEPAVQPARMTLAELCDHIEQTHHALLRRELPRLAAM
                     IEKVLNAHGQSYPWIQALQQVFHGLADELFSHMMKEEQILFPLVRKLEAGDPSAAPMI
                     AAEHGPIHCMEAEHDSAGDALARMSELTVVYTPPQGACNTFRAMLDGLRELELDLHQH
                     IHKENNILFPRAQEQARALV"
     gene            complement(8388..10766)
                     /locus_tag="PLS90_08700"
     CDS             complement(8388..10766)
                     /locus_tag="PLS90_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009578910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HPK02523.1"
                     /translation="MRYFKLWLALGLVIVASFAVLGYFGFEIYRQMPPIPAEVVDADG
                     NVLFTGQDIRDGQNVWQSIGGQELGSIWGHGAYVAPDWSADWLHRESVLILNAWARAE
                     HGAPYADLDERAQSDLRTRLRTLIRTNTYDPDTQRLVLNNDRAAAIERLGRYYGALFS
                     DAAEFPADVQEIADGGFNPAELRDAYAVATNTIRDPERQRQMNAFFFWTAWAAGAERP
                     RATEAAAVQSFSDRPAITYTNNWPAEALIANRPSGAIIVWSVVSFVLLLAGIGALAWY
                     YAVLKHRDDPETNLPVGDPLLGLQPTPSMKATLKYFWVVVALIVAQVGLGAVTAHYGV
                     EGSGFYGFPLADILPYAVTRTWHTQLGIFWIATAWLATGLFMAPAVSGHEPKFQRAGV
                     NFLFVCLLIIVVGSMFGTWYGTRQTMGYTMNFWFGHQGYEYVDLGRFWQLFLFIGLVL
                     WLVLMGRGMWPAFRQPGEHRHLLGLFLVSSAAITLFYAAGLMWGRQTHLALAEYWRWW
                     VVHLWVEGFFEVFATVVIAFLFTRMGLLRTATATAAVLFATTIFLSGGIIGTFHHLYF
                     TGTPTAILALGAVFSALEVVPLVLIGFEAYENLTLSRVRPWVNAYKWPIYFFIAVAFW
                     NLVGAGLFGFLINPPIALYYMQGLNTTPVHGHTALFGVYGMLGMGLTLFCLKGMAARN
                     VWRTRVLSFAFWSINIGLALMVLLSLLPIGLMQTWASVEYGLWYARSAEFLQNGLMDN
                     LRWLRVVGDTIFAIGIVAFGWFVLGLKMGWSVDSGRVDLSTEQYPQGAVAGK"
     gene            10933..11373
                     /locus_tag="PLS90_08705"
     CDS             10933..11373
                     /locus_tag="PLS90_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPK02524.1"
                     /translation="MFSQTAEYALRATVALAQQPDNPLTTAEIARITKVPAGYLSKVL
                     QMLAREKIIRAIRGLHGGYQLLRPAEELTIFDVVNAVDPIKRIHSCPLGLAAHGAQLC
                     PLHRRMDDAMAAIQEALSATTLAEVVAEPSASIPLCPGISFVAD"
     gene            11430..>11916
                     /locus_tag="PLS90_08710"
     CDS             11430..>11916
                     /locus_tag="PLS90_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02525.1"
                     /translation="MPEPAPPLAAAAPAAAVDIPLPHRPYFLAAIATVLTAGASWGAW
                     LLWSIGFAGDFTGISLHHVNAHGHAQIFGWVGLFVMGFGYQMLPRLWHAPAAAPRGAG
                     TALGLMLSGLVLRTAGMSAPAAGWAPPAALGGGALEIAAIGVFAGHLAGTLRAARRPL
                     EP"
BASE COUNT         1778 a         3799 c         4516 g         1823 t
ORIGIN      
        1 cgcggggact gcctgctgca caaggaggat gcgcgcgtca gcgagtgctt tgtgcatgct
       61 ttcgagaacg aagggatcaa ggtccatctg gaggtggaag tgagcgaagt gcagtacgac
      121 ggagcggcat ttcacctgaa gctggtcggc gggacgcggc tggagggcga cgcgctgctg
      181 gtggccgtcg gccgccggcc caattccgcg gcgctccagt gcgccaggtc cggcatcgaa
      241 ctgacggatg agggattcat ccaggtcgat gagcggctca ggacgacgtg caagggggtg
      301 tttgccgccg gggaagtggc ggggcagccg ccgtttaccc atgccgcctg ggaggaccac
      361 ttgcgcattc tggatacgct ggaggggggc aaacggacgc gggcggatcg ggcgctcggc
      421 tactgcacgt tcaccgagcc gcagatgggc cgcgccgggc tgtcgatgaa gcaggcccag
      481 gcggcgggct atcgcgcgcg gtgcgttggc aaaccgatcc gcgagatcgc ccgcgcggcc
      541 gaatggtgcc accacgaggg ctttttcgag atggtgatcg atgaggagtc ggaccggatc
      601 ctgggcgcca cgctggtcgg ccatgaggcc ggcgaactcg tccatctgtt cctcggcctg
      661 atcgaatgca aggcgacatg gcgcgtgctg gccgacatgg tggcgatcca ccccacctac
      721 ggcgaagggc tgcggagcat ggcgcgccag cttgccgact gagcggcggc ggtcccgctc
      781 cggggggaat tcaccggtca cgtcgcctga gttatgatgc atgcagccaa ggccgcggcc
      841 acttggcgca aatcaccgca ggggggagac gaccatggac gtcgggcagt tgggtgagca
      901 actggtgcaa tggctcaatt tcgagtcgcg gccactggat gtcatcctgc ggctgtcgat
      961 ggcggcgctc tgcggcggca tcatcggctg ggaccgcgaa cgcgaggaca agccggccgg
     1021 tctgcgcacg cacatgatgg tggcgctggg ggccgcggcg tttatggtgt tgacggtcga
     1081 agtcagccgg cagacgaccg ggttcgcgta cgatccggtc cgcacggtgg ccggcatcgc
     1141 cggcggattg gggttcctcg gcgccggcac gatcatccag tcgcgccatc gcgtcgaagg
     1201 ggtcaccacg gcggcgggca tctgggtggt gggggcgctg ggcacgatct gcggcgcggg
     1261 tctctacggc gtggcgctgc tgaccacgat cctggcgttc atcattctga cggtggtgca
     1321 gttcttcaag ctgcgcgtgc tgcatctgag ggcacagccg ccggcgcaca agccggactg
     1381 acccgcgcgg cgcggcgagg ggagcgtcag ccgcgcgccg tgcgcgcgat ctcccgcgcc
     1441 tggcggcaga gcttggtaat cgaggcccac tcctgccggg cgattgcgcc ggccggggcg
     1501 atccatgtcc cgccgacggc cgttacgatg tccagcgcca ggtaggccgc gaaattgtcc
     1561 tcgcggatcc cgccggtcgg caggaacttg accccggtgt ggcggaacgg cgccgccagc
     1621 gccttgagca tctcgatgcc gccagagggc tcggccggaa agaacttgag cacctgacac
     1681 cccgccagca gggcgcggtc gatctcggtc ggcgtgacca cccccggcac gaacgggagt
     1741 tgcagcgcgg cacaggcttg cgccacgtcc gggttgaagc cgggcgcgac gatgaaccgc
     1801 gcgcccgcct ggtgcgcctg ccgcacctgg tccggcgtgc ggacggttcc ggcgccggcc
     1861 agcacgtcgg gacactcccg gcgaattcgc gcgatcgcgt cggccgcggc gctggtgcgg
     1921 aacgtcagct cgatcaccgg gatctccccc tcggccagcg cccgcgccag cggcacggcc
     1981 agctcggggt cttccatcgc gatgacgggg accaggccgg tccgctcgat ctgctccaac
     2041 gcctgattct gcatgcgcgt gctttctgtt ctggaattga gttgcccgcg aattctgccc
     2101 gaaccgcgcg gcaatgacca ttcttccctg gctttcggaa gctccggcat ccgtcgggcc
     2161 ggggttacca gccctggtcg tgttccaact gcttgcgggc ctgctcgcgg gcggcttcct
     2221 tctcccggcg gcggcgcgcg agcatctcgc gctcggtacg ccgcgcttcg cccaggtaga
     2281 gcaggctggc gatgctggcg ggcagcgtgc cgagggcgat cccgccgagg atcagcagcc
     2341 aggtttcgtc gccatcgaga tggaatcccc gctcgccgag gccccgggcc gccacggcca
     2401 gcccgaccag gagcatcagg gcccagaacc cggccagcgc gatcggatag gaataccacg
     2461 gcgaacggcg atcataatcg ccctgctgct cgccgatcgt cgagaagcgg tgcatcacgc
     2521 ggccgggctg cacgggcggg gggcgcaggt tttcgtagtc ccgccacagg tcggcgggaa
     2581 ccggcgcata gccgtcgggg gcgacggacg gatcggggcg ttcgtgatcg atgtagcgga
     2641 ccttctcgcc gccgccgaag ggcggaagag ggaacaggcg aggtggccat cgcgccaaca
     2701 tgatgccctc gatgcccgga acgaccgctg catggatgat agggactggg caacgcgggg
     2761 gacaagcgga atcccggtca tgaattgcgc agagacgttt gggtcgcggg tggaaaaagg
     2821 ttgggaaatc gggggcgcgg gggggaagat ggggcgcgca tgccgtcgcg cccggcggcg
     2881 cgccaccccc cgatgtcggc cgtctcgcgc cttgttacgc tacgatctcg cttacgccct
     2941 cgcgcttccg gcgctgatgc cgtatctcgc ctggcggcgc ctcgcgcgcg gcaaatacgc
     3001 cgagagcgcc ggcgggatgc tggggcgccg gctgccgccc ccggccgaag cgcggcggtt
     3061 cgccgacggc tcgctgtgga tccacgccgt ttcggtgggc gaagtgcagg ccgcgcgcgt
     3121 cgtggccgat gggctgcagg cacttgcgcc gcggttgccg ctggtcatca gcacgatcac
     3181 cgagacgggg caggcggcgg cccggcgatt gtttcccgat gcggtccaca cgtattatcc
     3241 ggtggatttt tcggtgaacg tcgagcgctt ccagcgggtg ttcaatccca ggctgttcgt
     3301 cctgctcgag agcgacctgt ggcccaattt tctggcgcat gcgagccggc gggggacgcg
     3361 ctgcgtgctg ctcaacgccg acatctccga ccgctcgttt ccgcgctacc tgcggtttcg
     3421 ccgctacctc gggccggcgc tgggggcgct cgacgccgtc tgcgcccaga ccgaggtcga
     3481 cgccgagcgg ttcaccgcgc tgggcgtcgc gccggcgcgg atccgcgtca ccggcaactg
     3541 caagctcgac ctgcccgcac aggtgcttga tgagcgcgag cggcgggaac tgtgcgcgcg
     3601 gttcggcctg aatccggagc ggcggtggat cgtcgccggg tcgacgcatg aaggcgagga
     3661 agcgctgatc ctgcgggggt tcgcggagct gcgccgcgag atgccggggc tggggctgct
     3721 gatcgcgccg cggcatcccg agcggttcgc ggcggtggag caactggcgc gcgacgcggc
     3781 gccggcgggc gaagggtggc gcgtcggcgc ggccagccgc cccgagccgg cgagcgatcc
     3841 cgacgtcgtg gtgctcgatg cgatgggggt gctggcgcgg agttacggtc tcggcgcggt
     3901 cgcgatcgtc gccggcagct tctgcccgac cggcgggcac aacctgctgg aggccgccgc
     3961 gcacggcgtg ccggtggttt acggccccga catgcacagc cagcgcgaga tcgaccggct
     4021 gttcaagtcg atgtgttcgg gcttgcaggt gcggcccgac gagctgcacg cgacgctgcg
     4081 gcgactgctg acggacgaag ccttccgcgc ggccgagggg cgcaaggcgt tgcaggtgct
     4141 cgaacgcaac caggggagcg cgcggcggac gaccgaggcc gcccgcgaga tcctgctggc
     4201 gaccggccgc tggccgtaag ggcagggatt ccgacggatc ggacggattt cgagggacag
     4261 caaacgggtg gcctgcgggc tacggttctt cctcgcgcgc gagccattgg acgatgcggt
     4321 cggtgatcca ggcgtgaggc gtcgggatgg ggggcgggac gtttgccggg accggtcgcg
     4381 cgcgcgcgcg atccagtcgc tcccgcagcg cgggcgagtt cagttcatcg ggcgtgatcg
     4441 cgtggtccgc gcgatcggga ttgcgctcgc ccagcgtgag cacggggatc gcgcgctgga
     4501 gggcttcgtc ggcgatcagc ggcgagtcgg tcaggacgag ctccgcccgg ccgagcagct
     4561 cgcgccagag cggataaggc aggggcggga cgcgcagcag gttggcgggg cgtcgttcga
     4621 gcgcgcggaa cggcccctcg aggcgcatgt tcagcgtgct ggagatcacc caatcgcgtt
     4681 cgggcgccgc ctcggcccag cggcccgcgg cgtgaatcaa gcgcgaggcg gcattgccgt
     4741 gggtcacgcc ccactcgcgg cggtagatgg ccaccagcgc cagcggggcc gcgtcgcgca
     4801 aaccggggat ttcggcttcc aacggggaac tgggcggggc cgcggccagt ccctcggcga
     4861 cggcgtcggg catcgccggc acgtcgagga tcgcgacgcg cggcgcgagc gccgcgatca
     4921 cgcgcgccag cccggcctcc cagtccagtc ggccgatgat ccccaagcgg tcggcgggcc
     4981 gcagccagag gccgcggcaa ccgcgcccgt ggcagcacag cgcggcggcc gccgccgtcg
     5041 gaccatatcc cgcgaagacg acgtgcgacg cccggcgcaa gtgaacgaac gattcggtcc
     5101 gctccagcat cagccgcagc cggaccgcgg ggtcgtcggc cgggtggcag agcgcgccgg
     5161 tctcgtcggg cgccagcgcg agcgcatcga gggtctgata cgccgccgtt ccgtgctcgc
     5221 cggtgcaggc cagccagccg accgcgcgcg gattgccgcc ctcggccggc gggcggcaga
     5281 gcgccttgaa cgatgggccg agcaggagga tctccccgcg tgtcccggcc agccagacca
     5341 cacgctgggc gcgctggctg ctcatggagc cgacagatcg cggcccatca gcccggcgta
     5401 gacgcgggcg ccggcttcca gctcggcgat ctcgatgaat tcgtcctcgg tgtgcgcctg
     5461 gtcgatgctg cccggtccga ggatgatgaa gggaatgccg gcgtggccca gttcgcaggc
     5521 gtcggtggcg tagtcgacgc cgatgggcgc ggtggggcgt ccggcggcgc cggtgacggc
     5581 ctgcgccgcg cggaccagcg cgctgtcggt cggggcctgc acggcgcggg cgtcgagcgt
     5641 cacttcctcg atgcaccctt cgaattgggg aaagagctgt ttcgccgcgg cgaacagccc
     5701 ctccagttcg cggcggagat cgtcggtcgt ctcgccgggg atcgtccggc ggtccacatg
     5761 catgcggcag gaagggggga cgagattggg ctgcacgccg ccgacgatct ggccgacgtt
     5821 gatcgtgggc gagccgacga gcggcgcgct gcgccgcgcc agttcgggga cgtattgctc
     5881 gtcgatgacg cggacgagcg ccgacgccgc cgagatcgca ttgatcccca ggtgggggcg
     5941 cgccccgtgc gccgatttcc ccgccacgcc gagccacaac cgcacgcttc ccttgtgggc
     6001 ggtcacgatg cgcaggctgg tcggctcgcc gatgacgatc ccgacggcct ccaggccatc
     6061 ggccaggagg gcccgggcgc cgccgaagga gaactcctcg tcatgaacga gtaccagttc
     6121 gacgtcgtgc ggggggggcg cgtcggcctc cgcccacgcc gccagcgcgt gcatcattgc
     6181 cacggcctgg cccttggcgt cggtcgagcc gcggccccac aggcggcccc ggtcgacgcg
     6241 gccgccccac ggttcgatct tcatgccggt gacggcgacg gtatcgacgt gggtttcgaa
     6301 gacgagcggc gggccgggcg ttcgtccgcg cagccgtgtg cggaggagcg ggcggccggg
     6361 ttgcgtttcg accaggcgcg gcgtcaggcc ggccgaggcc aggatcccgg cggcgcgctc
     6421 cagcacctgg cgctcgcccc cctgcggccc gccgaagccc ggattcacgc tgttgatctg
     6481 gaccagcgcg gccaggcttt cggttagttt gtcgggctcc agccagcggg agcggcccgg
     6541 cggcgtcggg ctcatgacgg gaagttcgga ggtcggggtc tggcggcggg cctgcgcggc
     6601 atggcggtga cgctcactcc ttttcctcgg cgggggcgtc gctgccggcc tgctgcatat
     6661 cgccggcgag cgcgcggatc tggtcgcggc actcggcggc gcgggcgaag tcctcgctgg
     6721 cgatcgcctc ctgcatttcg cggcgcagct tgcggatcag gtgctcgcgc tcggccttgg
     6781 ccgggtcggc ctgcttcttc tggaccggct tgtcgggggc ctgcgggatc ggcggcacgc
     6841 tcagttccag gctgccggcc gatttgcgat ggcgcgccgg cgcatcctgc tgatcgggca
     6901 cctggcgtcc gccccccggt ttgcggccga tgtggcgcgt ctcgccgtgg aaccggcgca
     6961 ggtcggggac gaggtattcc tcgaaggact cgtagcaggc gtcgcagccg agcatcaggt
     7021 tgcgcttgta ggcctcgaag cgcaggccgc actggctgca aacgactccg gccggggcgg
     7081 tcgagccgct ctgggccttc acctcctgct tgagcagccc ctcgaggatc tcgctgagtg
     7141 gaatttccgg tttgggaata tactggctgg tttcggccgc gtgccgatcg cacaggtaga
     7201 cgtcgaagat ctggcccttc tcgatgcgtg tgaacttatg cgtcgccggt tccccgcatt
     7261 tcatgcattt tttgttgccg ttcatatcgt tctctggcgc tccctgatgg tatcacccca
     7321 cggcagccgg ttacttccca atataaacca atcccccggc cgtcgcacgt gcaaaggcaa
     7381 ggagattggc gtgggcgtcg agggggcgga ggcagccggc ccatgcgccg gggcggcggc
     7441 ggaagggatt ttccagcgcc tcggggtcat ggccaggatg gccatgccac gtggtggagc
     7501 gtgagggagt gaagctggat tgaccggcgg cggcgccggt cgcacgccgg cgtggcgccg
     7561 attcccacta cggggttccg gcgccccgcc gggatgcggg ctggacgggg cctagaccag
     7621 ggcgcgggcc tgttcctggg cgcgggggaa gaggatgttg ttctccttgt ggatgtgctg
     7681 gtggaggtcc agctccagct cgcgcaggcc gtccagcatg gcgcggaagg tgttgcaggc
     7741 cccctgaggc ggggtgtaga cgaccgtcag ctcgctcatg cgcgccaggg cgtcgccggc
     7801 cgagtcatgt tcggcctcca tgcagtggat cgggccgtgc tcggcggcga tcatcggggc
     7861 ggcggagggg tcgccggcct ccagtttgcg cacgagcggg aagaggatct gttcttcctt
     7921 catcatgtgg ctgaacagct cgtcggccag gccgtggaag acctgctgga gcgcctggat
     7981 ccagggatag gactggccgt gggcgttgag gaccttctcg atcatcgcgg ccaggcgggg
     8041 gagctcgcgg cgcagcaggg cgtggtgcgt ctgttcgatg tgatcgcaca gctcggccag
     8101 ggtcattctg gcgggctgga cggcgggctc ctcggggccg gcatcggcgg cctcgatgag
     8161 ctgcaggacg tcctcgacac gcaggttgcg ccgggcgcag acctcggcca gcggcttctt
     8221 gccgccgcag cagtagtcga tgcccagttt ttcgaagacg cgtgagcggg cggggcgttc
     8281 ggcgacgaag gcgccgacgg tcatctcggg gttgagtgta gcggtcatgg acgactctcc
     8341 ttgattcaaa cgggatggag gacgccgccg gcggggcggc gcccgcgtta cttgccggcg
     8401 acggcgccct gggggtactg ctcggtgctc aggtcgacgc ggccgctatc gaccgaccag
     8461 cccatcttca agccgaggac gaaccagccg aaggcgacga tgccgatggc gaagatggtg
     8521 tcgccgacga cgcgcaacca gcgcaggttg tccatcaggc cgttctggag gaactcggcc
     8581 gagcgggcgt accacaggcc gtattcgacg ctggcccagg tctgcatcag gccgatcggc
     8641 agcaggctga gcaggaccat cagcgccagg ccgatgttga tcgaccagaa ggcgaaggag
     8701 aggacgcggg tgcgccagac gttgcgcgcg gccatgccct tcaggcagaa gagggtcagg
     8761 cccatgccga gcatgccgta gacgccgaac agggcggtgt ggccatggac gggggtcgtg
     8821 ttgagcccct gcatgtagta gagggcgatc ggcggattga tcaggaagcc gaacagaccg
     8881 gcgccgacca ggttccagaa ggcaacggcg atgaagaagt agatcggcca cttgtaggcg
     8941 ttgacccacg ggcggacgcg cgagagcgtc aggttctcgt aggcttcgaa gccgatgagg
     9001 accagcggga cgacttccag cgcgctgaag accgcgccga gggcgaggat cgccgtgggc
     9061 gtgccggtga agtaaaggtg atggaacgtg ccgatgatgc cgcccgagag gaagatcgtg
     9121 gtggcgaaga ggacggcggc ggtcgccgtc gccgtgcgca gcaggcccat acgggtgaag
     9181 aggaacgcga tgacgacggt ggcgaagacc tcgaagaagc cctcgaccca caggtggacg
     9241 acccaccagc gccagtattc ggccagggcc aggtgggtct ggcgccccca catcaggccg
     9301 gcggcgtaga acagcgtgat ggcggccgag ctgaccagga acaggccgag cagatggcgg
     9361 tgctcgccgg gctggcggaa ggcgggccac atcccgcggc ccatcagcac gagccacagg
     9421 accaggccga tgaacaggaa gagctgccag aaccggccga gatcgacgta ttcgtacccc
     9481 tgatggccga accagaagtt catcgtgtag cccatcgtct ggcgggtgcc gtaccaggtg
     9541 ccgaacatcg agccgacgac gatgatgagc aggcagacga agaggaaatt gacgccggcg
     9601 cgctggaatt tcggctcgtg gcccgagacg gccggggcca tgaacaggcc ggtggcgagc
     9661 caggccgtgg cgatccagaa aatgccgagc tgggtgtgcc aggtgcgcgt gacggcatag
     9721 ggaaggatgt cggccagggg gaagccgtag aagccggagc cctcgacgcc gtaatgggcg
     9781 gtgacggcgc ccaggccaac ctgcgcgacg atgagcgcga cgacgaccca gaagtacttg
     9841 agcgtagcct tcatcgaggg ggtgggctgg aggccgagga gcgggtcgcc gacggggagg
     9901 ttcgtctcgg ggtcgtcgcg atgcttcagc acagcgtaat accaggccag tgcgccgatg
     9961 ccggccagca gcaggacgaa gctgacgacc gaccagacga tgatggcgcc gctggggcgg
    10021 ttggcgatga gcgcctcggc cggccagttg ttggtgtagg tgatggcggg ccggtcgctg
    10081 aaggactgga cggcggcggc ttcggtcgcg cggggccgct cggcgccggc ggcccatgcg
    10141 gtccagaaga aaaaggcgtt catttggcgc tggcgctcgg ggtcgcggat tgtgttggtg
    10201 gcgacggcgt aggcgtcgcg caactcggcg ggattgaagc cgccgtcggc gatctcctgg
    10261 acgtcggcgg ggaactcggc ggcgtcgctg aagagggcgc cgtagtagcg gccgagacgc
    10321 tcgatggcgg cggcgcggtc gttgttgagg accaggcgct gcgtgtcggg atcgtaggta
    10381 ttggtgcgga tgagggtacg caggcgggtg cgcagatccg actgggcgcg ttcatccagg
    10441 tcggcgtagg gggcgccgtg ctcggcgcgg gcccaggcat tgagaatcag gaccgactcg
    10501 cgatggagcc agtcggcgga ccagtcgggg gcgacgtagg cgccatggcc ccagatggag
    10561 ccgagttcct ggccgccgat cgactgccag acgttctggc cgtcgcggat atcctggccg
    10621 gtgaagagga cgttgccgtc ggcgtcaaca acttcggccg ggatgggggg catctggcga
    10681 tagatctcga atccgaagta tccgaggacg gcgaacgagg cgacgatgac gagaccgagc
    10741 gccagccaga gcttgaagta gcgcatgagg aggaaaacct ccgggggggg tggatcgccg
    10801 gcggggggcc gggaaaaagt ggatgctttt atccacttga cggcgagcat accgcgccgc
    10861 taaagtggat gtcaaggtta agatatccat tttttcctgg atatcgcagt tccccctgaa
    10921 agctcctcct gcatgttctc tcagaccgcc gaatacgccc tgcgcgccac cgtcgccctg
    10981 gcccagcagc ccgacaaccc gctgacaacg gccgagatcg cccggatcac gaaggtcccg
    11041 gccggctacc tgtccaaggt cctgcagatg ctggcgcggg agaagatcat ccgggcgatc
    11101 cgggggctgc acggcggcta tcagctgctg cgccccgccg aggaactgac gatcttcgac
    11161 gtggtcaacg ccgtcgatcc gatcaagcgg atccacagtt gtccgctggg tctggcggcg
    11221 cacggcgcgc agctctgccc gctgcaccgg cggatggacg acgcgatggc cgcgatccag
    11281 gaggcgctgt cggcgacgac gctggccgag gtggtggccg agccgtcggc cagcatcccg
    11341 ctgtgtccgg ggatcagctt cgtggcggac tgaccgccgc aacctgattc gcgtatcaag
    11401 ccgcaaaacc cctccgccgg agcccgccca tgcctgaacc cgccccgccg ctcgcggccg
    11461 ccgcgccagc cgccgccgtc gacatcccgc tgccgcatcg gccgtatttt ctggccgcca
    11521 tcgcgaccgt cctgaccgcc ggcgcctcgt ggggggcctg gctgctgtgg agcatcggct
    11581 ttgccggcga tttcacgggc atttcgctgc atcacgtcaa cgcgcacggc cacgcgcaga
    11641 tcttcggctg ggtcgggttg ttcgtgatgg gatttggata ccagatgctt ccgcggctgt
    11701 ggcacgcccc ggcggcggcg ccgcgtgggg cggggacggc gctggggctg atgctgagcg
    11761 ggctcgtgct gcgcacggcg gggatgagcg cgccggccgc cggctgggcg ccgccggcgg
    11821 cgctgggggg cggggcgctg gagatcgcgg cgatcggcgt gttcgccggc cacctggcgg
    11881 gcacgctgcg cgcggcgcgg cggccgctgg agccct
//
LOCUS       DAOPCO010000103        11861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5331_length_11861_cov_5.124428, whole genome shotgun sequence.
ACCESSION   DAOPCO010000103 DAOPCO010000000
VERSION     DAOPCO010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11861
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5331_length_11861_cov_5.124428"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..2224)
                     /locus_tag="PLS90_08715"
     CDS             complement(206..2224)
                     /locus_tag="PLS90_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02526.1"
                     /translation="MRLNRHRQQREAEVARGVDPNANAAGTPAPDPVPVTLWISDAEG
                     SRIYLGREWHGLARVRPGEESGNGWLEAIHRDDRAEVATRLEQATARQQAFSVEYRLR
                     TETGDYRWMLDSGRPRYALDGTFLGLAGTVLDVTERRETLEALRKSERRYRQLVSLLP
                     VAVFACDAKGQLQYCNTRAVSLWGREPKLNCSEELYCGSHRLYYPDGAPMSRDECPMS
                     QAVRRGQSFREKELIIEQPTGVRVLVSVNIDPLLDEDENIVGAVSVFQDVTARKRTEE
                     ELRTLTEELERRVRERTAVAEQRARQLQAVTLQLTEAEERERRRLAGVLHDGLQQSLV
                     AARLSASLIKTRLESRAALLELAAELDAILAQCIQESRTLSHELVPSILYETGLCAAL
                     KWLARHVKEKHGLAVRVTAGEEPLLSDLTRSFLFQSVRELLFNVVKHAGVMEAWISAG
                     NDEQGWRLEVADGGQGFNVANAMGVDVDRGFGLCAIRERIGLMDGEMEIHSEPGKGTR
                     IILRVPLESTAIRSQQAINPVMLEETPPREGTSGAAAEPGMIRVLLVDDHRVMRSGLE
                     SLLEEEDDIEVIAGASNGLEAVDCARRLKPDVILMDVSMPEMDGVEATRVISAEFPEI
                     RIIGLSMHEEADMAHRMLEAGAAAYFSKGGAPDMLIDAIRDLEATPLR"
     gene            2801..3802
                     /locus_tag="PLS90_08720"
     CDS             2801..3802
                     /locus_tag="PLS90_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPK02527.1"
                     /translation="MLPLETLIDDERRRLDAPLARRVLEAVKLLNRLSLGSEHLRRNL
                     AELGGARTKEDRSPVTVADLLHQGLVQQWIARLFPGDGLICEEPRSLQEAVMHEAQAA
                     ADRFYGMPLEDRLPDLPERAEVTWILDPIDGTKGYLAGRYYAIALGFFVGDQPWFGAM
                     AVPPAPAMDLRISRRIGFAISGVGGWVCDIVEAENLMCQKLERAEATPRDPVRVAMSL
                     EHGKTDERIKPAGEDDHARIEIVKLDSQAKYLAVALGEIDAYLRDRRDDGQSDVAWDH
                     MPGAVIARAAGCSVTHYDGGEVRFEPQRELSLKGGLACYRGGNLGPVGKLVQRLVQR"
     gene            3799..6096
                     /locus_tag="PLS90_08725"
     CDS             3799..6096
                     /locus_tag="PLS90_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02528.1"
                     /translation="MSAGRGLCRLIGLSLLAAWAAPEARSQPAPAPNDELTRVDDYAS
                     SALRDLFSNIPHGFPRFEIEGRARESRLLTHLLWYHFINRPGNGRALFNKEYLTLADM
                     WLGEAAVPRQQNQLIQEIHRENISAVKLDAEGYVHSHQHFSHAHEHGWPFPMWTQAEA
                     NPDAIRGIAAGWHFQRLEDNAPGWVWDYLRSWGHPEYYGEGAAASFELVNLESRGIAN
                     QRWHLRATGPSPALLTPAGMPIDAFNAPFLQIRWKRSGEPPHHRRPYVEWLRDEDQEW
                     SPQRRVHIYFDHEEHAEGTDLVFSMIEMYRHPLWRGQIKRLRFCLAPGETGVEFEIDS
                     IFTVYDTRHTINNPIFILACWNTFRWTGDLAFLRANINRMRLALRYQQYAMSALELGH
                     IRNPWVGHDGLAGWQLGSEGGKDIQSGHGIGNNYWDLMPFGRDDLYATSQYHAATLAM
                     AEVEEAVLDHPGWDLPRGPLGLDPTLLRRYAEEVRQTANALFWNPETGRFHAAIDAEG
                     KPHDYGFTYLNLEAIWYGLASEEHAAQIMSWINGDRFVVGDTSMGKDLYAWEFAPRTT
                     TRRNLEWYGPTWTNPEEIAWGDQVQDGGAVLGFSFFDLWARLRVLGPDNAWNRLGEII
                     DWQERVWRQGDYRDYYAAAGRGKLQGCGTPGGLGIDCEFYESSLLPAIVVYGFLGLDP
                     RADCLAIHPRLPGATPAMTVRNVLYHWTPLDITAGGDSIEITLKETPLDPIQIQVPEG
                     YRLAESDAAGPLFQIAKAGAYRFER"
     gene            complement(6139..6360)
                     /locus_tag="PLS90_08730"
     CDS             complement(6139..6360)
                     /locus_tag="PLS90_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HPK02529.1"
                     /translation="MRKEYDFTNSKRNPYAIHFRKQVTLRLGTDVIEYFKAMAQETGV
                     PYQNLINLYLRECARSGRKLALKWVKQAS"
     gene            complement(6357..6647)
                     /locus_tag="PLS90_08735"
     CDS             complement(6357..6647)
                     /locus_tag="PLS90_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015078625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HPK02530.1"
                     /translation="MSSIRFTWDPAKAELNSRKHAVSFEEASTVFADELGRLMYDPDH
                     SNDEDRYLILGMSTSLRILVVCHVYREDAGLIRIISARKATSLERKQYEDLP"
     gene            complement(6867..8369)
                     /locus_tag="PLS90_08740"
     CDS             complement(6867..8369)
                     /locus_tag="PLS90_08740"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK02531.1"
                     /translation="MLRESQYILRQVNIALDLLLGAAAFLIAHALRALLSIYIVPGFV
                     SPSTLGYYLWLLPAGPLAIVLVLAYNGAYRDLRLRIRTAEQARRIATSCFEAALILVG
                     IDFLFGGVEFLGWRLRPAEAANTSRAILVLFPLVAFALLTGKAWLLRRFLMAMRRRGL
                     HDRRVILVGSGAALSEFAETIAHHPIWGLNIVGIVTDRPDCQGAGFDPDRVPESTLGW
                     PILADLERAPAVLWRTPIDEVIFLPESAPLASLRPLLEICEEMGVRTHLPLNFFSARI
                     AHPVIDRFEETPVLSYWPTQPIGPALLFKYAFDRIAAVILLVLASPVMLATAIAIRLT
                     SRKGEPIIFSQQRVGLNGRLFTCWKFRSMRSDAEMERETLEQLNEQQGPVFKMRHDPR
                     ITPLGRFLRKFSIDELPQLWNVVRGEMSLVGPRPPLPQEIEKYDRWQRRRLSMKPGLT
                     CIWQVSGRNLLPFDTWMKLDLEYIDNWSLLLDFKILARTVYVVLTGYGAM"
     gene            complement(8529..10067)
                     /locus_tag="PLS90_08745"
     CDS             complement(8529..10067)
                     /locus_tag="PLS90_08745"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HPK02532.1"
                     /translation="MAGADDTNAPKPIRITELLLRDAHQSLLATRMRTEDMVPICEQL
                     DKVGYWSLEVWGGATFDSCLRFLGEDPWERLRTLKKHLPRTPLQMLLRGQNILGYRNY
                     ADDIVERFVDRSIANGMDVFRIFDALNDVRNLKTAVRAVLKGGAHAQGAICYTVSPVH
                     TLEKFVQTARQIEDLGCQSLVVKDMAALMTPVPAYELVRALKEAVKIPVHVHTHATTG
                     VAPMVLLRAIEAGADGVDTAISALSLGSGHVPTEAIVETLRTTPHDTGLKQEDLLPIA
                     DYVRKIRPKYSEFESTFTGADPRIFVSQIPGGMLSNLESQLKKMNCFDRIDEVLEEVP
                     RVRKEMGYIPLVTPTSQIVGTQAVMNVLTGNRYQTVPEESRNIFAGRYGETPAPVDPE
                     IQKKVLGDEQPITVRPADLIPNEWDKIRQEVEGKARNDEDVLSYAIFPKVWLDFYEQK
                     INPRPAAPAPSPAPVAAPAPPPAPAAPQPQAAAAAVHNGRPLRMTIAVGGRSFQAEVA
                     ILEP"
     gene            complement(10147..11142)
                     /locus_tag="PLS90_08750"
     CDS             complement(10147..11142)
                     /locus_tag="PLS90_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HPK02533.1"
                     /translation="MPKFKALWVEEQTGGVFIRRIVERDSDDLPDGDVLIEVHYSSLN
                     YKDALSAHGNRGVTRAYPHTPGIDAAGVVVASRNGAFREGDPVIVTGRDLGMNTAGGL
                     AGMIRVPADWVVPLPDGLTLRDAMIIGTAGFTAAQSVWRLEEHGVLPGSGAVAVTGAT
                     GGVGSFAVALLAHLGYRVAAITGKRAREPWLREIGAAEVLGREEFMTDAARPLLSARW
                     AGAVDCVGGEALSILLRSTHRRGAVTTCGMVAGPELHVSVFPFILRGVNLLGIDSAEC
                     PNPMRREVWRRLAGPWKIPSLDALATEIPLEEAIPALEAMIRGESVGRRVVRTRL"
     gene            complement(11187..11666)
                     /locus_tag="PLS90_08755"
     CDS             complement(11187..11666)
                     /locus_tag="PLS90_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPK02534.1"
                     /translation="MAGCARPQTRQMLATAYCGCGECNGYTRGSWKFLKLDFWNRYVT
                     ADWGKGQKYTGRTANGMRPHPPRPGLLSWNTLVHPWMAAPRLLLPWLILRQPGTIAAD
                     TDYYPFGTEMVIPGWGWGRVEDRGSAIQGPDRIDLFHRSHGKANDWGRRWVTVEMVD"
BASE COUNT         1965 a         3798 c         4059 g         2039 t
ORIGIN      
        1 cgcgtccctc gccctcggct ctcgaaattt gtttccttct ttcccgccac tgaagtggcg
       61 ggctgaagtc tgccgtccct gataggactg gcggtgggac gattccggca cgccctcggc
      121 cgctcgaatc ggtcgtgcgt ggccgcccat gagggactta ccctcaaatt cggttcccgg
      181 ttcgcgtccc tcgcccttgg ctctcctacc gcagcggcgt cgcctccaga tcgcggatcg
      241 catcgatcag catgtcgggg gcaccgccct tgctgaaata agccgccgcc cccgcctcca
      301 gcatgcggtg ggccatgtcc gcctcctcat gcatcgacag gccgatgatc cggatttcgg
      361 ggaactccgc gctgatcacg cgcgtcgcct ccaccccgtc catctcgggc atcgacacgt
      421 ccatcaggat cacatcgggc ttgagtcggc gcgcgcagtc gaccgcctcc agtccattgc
      481 tcgcgccggc gatcacctcg atgtcgtctt cttcctccag cagcgattcc aggccgctgc
      541 gcatcacccg atggtcgtcc accagcagca cacggatcat gccgggctcc gccgcggcgc
      601 cgctggttcc ctcgcgcggc ggggtctcct ccagcatcac cggattgatc gcctgctggg
      661 aacggatcgc cgtcgattca aggggcacgc gcaggatgat ccgcgtccct ttgcccggtt
      721 cgctgtggat ctccatctcg ccgtccatca gcccgatccg ttcgcgaatc gcgcacaggc
      781 cgaacccgcg atccacatcg acgcccattg cgttcgccac gttgaagccc tgcccgccgt
      841 cggccacctc cagccgccag ccctgttcat cgttccccgc cgagatccag gcctccatca
      901 cgccggcgtg cttgaccaca ttgaagagca actcgcgcac cgactggaac aggaacgagc
      961 gggtcagatc cgagagcagc ggctcctcgc cggccgtgac gcgcacggcg aggccatgct
     1021 tctccttgac gtggcgcgcg agccacttca gcgcagcgca gagccccgtc tcgtacagga
     1081 tcgaggggac cagctcatgc gacagcgtcc gcgattcctg aatgcactgg gcgaggatcg
     1141 cgtcgagctc ggcggccagt tccagcagcg ccgcgcggct ctccagccgc gtcttgatca
     1201 ggctggcgct cagccgcgcc gccaccaatg attgctgcaa gccgtcatgc agcacgccgg
     1261 ccagccgccg ccgttcgcgt tcctccgctt cggtcaactg cagggtcacc gcctgcaact
     1321 gccgcgcgcg ctgttccgcc accgccgtcc gttcccgcac gcgccgctcc agttcctcgg
     1381 tcagcgtccg cagttcttcc tcggtccgct tgcgggccgt cacgtcctga aacacgctga
     1441 ccgcgccgac gatgttctcg tcctcgtcca gcagcgggtc gatgttcact gaaacgagca
     1501 cccgcacgcc ggtgggctgc tcgatgatca gttccttctc gcggaacgat tgcccgcggc
     1561 gcaccgcctg actcatcggg cactcatcgc gcgacatcgg cgccccgtcc ggataataca
     1621 gccggtgcga gccgcagtag agttcctcgg agcagttgag cttcggctcc cggccccaca
     1681 gcgacaccgc ccgcgtgttg caatactgaa gctgaccctt cgcgtcgcag gcgaagaccg
     1741 ccaccggcag cagcgagacc aactggcggt agcgccgttc gctcttacgc agcgcctcca
     1801 gggtctcgcg ccgctcggtg acgtcgagca ccgtacccgc caggccgagg aacgtcccgt
     1861 ccagggcgta gcgcggccgg ccgctatcga gcatccagcg atagtcgccg gtctccgtgc
     1921 gcaggcgata ttccacgctg aaggcctgct ggcgcgcggt cgcctgttcg agacgcgtcg
     1981 ccacttccgc ccgatcgtcg cgatgaatcg cctccagcca cccgttgccg gactcctcgc
     2041 cgggtcgcac ccgcgccaag ccatgccatt cgcgaccgag atagatccgc gagccctcgg
     2101 catcggagat ccacagcgtc accggcaccg ggtcgggcgc cggcgtgccg gcggcattgg
     2161 cgttcggatc gaccccgcgg gcgacctcgg cctcgcgctg ctgccggtgg cgattcagtc
     2221 tcatgcggcc tgcgtcccag cgagggttac aaagaagaat gcgggaggtg cgcgtgcggt
     2281 ctggagggga tcgcagccct ccgtaatggg gtctgcttcg cccatcattt tgccacgcgc
     2341 ccgcaagacg cgtcacgcac attcaacccc cgaaacaaga ctcgtccctc gactctcgct
     2401 cgaaaattcg tttccgattc ccgggatcgc ctggatcggt tctgccgtct ctacgggact
     2461 ctgttgcgat ggggatcccg gttcccgcca ctgacgtggc gggctgaagt ctctcgtccc
     2521 tgacgggact gccctgttga aggatccggc gaggcctgga acgttcgaat cggtcactcc
     2581 ctccccattt tcatccctcg ttgtgaaccg cagcttcgtg agcaactcgg aattgtgatc
     2641 gaagaagcat tctggcgtct ctatggggct gccggtgggt cggttcggca agcgaccggc
     2701 cgtccggctc tcctggcaag cctgttggca gatcggggag ggttgcggta aactcgcagt
     2761 ttccgctcgc gcccacccct gtcaccgatt gaggaatcgc atgctccccc tggaaacgct
     2821 gatcgacgac gaacgccgcc ggctggacgc cccgctcgcc cgccgggtgc tggaggcggt
     2881 caagctgctc aaccgcctgt cgctgggttc ggaacatttg cggcggaatc tggccgagct
     2941 gggcggcgca cgcaccaaag aggatcgttc gccggtgacg gtcgccgacc tgctgcacca
     3001 ggggctggtg cagcagtgga tcgcgcggct gtttccgggc gacggcctga tctgcgagga
     3061 gccgcgctcg ctgcaggaag ccgtgatgca cgaggcccag gccgcggccg accggtttta
     3121 cggcatgccg ctcgaggacc ggctgcccga cctgcccgag cgcgccgagg ttacatggat
     3181 cctcgatcca atcgacggca ccaagggtta cctcgccggc cgctactacg caatcgcgct
     3241 ggggttcttc gtcggcgatc agccatggtt cggcgcgatg gccgttccgc ccgcgccggc
     3301 gatggacctg cggatcagcc gacggatcgg tttcgcgatc agcggcgtgg ggggctgggt
     3361 gtgcgacatc gtcgaggccg agaacctgat gtgtcagaag ctggagcgcg ccgaggcgac
     3421 gccgcgcgat ccagtccggg tggccatgtc gctcgaacac ggcaagacgg atgagcggat
     3481 caagcctgcg ggggaggatg accacgcgcg gattgagatc gtcaagctgg acagtcaggc
     3541 gaagtatctt gcagtggcgc tgggggagat cgacgcctat ctgcgcgacc ggcgcgacga
     3601 cggccagtcc gacgtggcgt gggaccacat gccgggggcg gtgattgccc gcgcggccgg
     3661 ctgttcggtg acgcattacg acggcggcga ggtccggttt gagccgcagc gcgagctcag
     3721 cctgaaggga ggcctcgcat gctatcgggg cgggaacctc ggaccggtcg ggaagctggt
     3781 ccagcggctt gtgcagcgat gagcgcgggt cgtggattgt gccggctgat cgggctaagc
     3841 ctgctggcgg catgggccgc tcccgaggcg agatcgcagc cggcccccgc gccgaacgac
     3901 gagctgacgc gcgtggacga ctatgcgtcg tcggcgctgc gggatctgtt cagcaacatc
     3961 ccccatgggt ttccaaggtt tgaaatcgaa ggccgcgcgc gcgagtcgcg cctgctgacc
     4021 catctgctct ggtatcactt catcaatcgg ccgggcaatg gccgcgcgtt gttcaacaag
     4081 gagtatctga cgctggcgga catgtggctc ggagaggccg ccgttccgcg ccagcagaac
     4141 cagcttattc aggaaatcca ccgcgaaaac atcagcgcgg tcaagctgga cgccgaaggc
     4201 tacgtgcact cgcaccagca tttctcgcac gcgcatgaac acggctggcc ctttccgatg
     4261 tggacgcagg ccgaggcgaa tcccgatgcg atccgcggca tcgcggcggg atggcacttc
     4321 caacgcctgg aggacaacgc tccgggctgg gtctgggact atctgcgctc gtggggccac
     4381 cccgaatatt acggcgaggg ggcggccgcc tcgttcgagc tggtcaatct ggaatcacgc
     4441 ggtattgcga accagcgctg gcatctgcgg gcaacggggc cgtcgcccgc gctgctgacg
     4501 ccggccggca tgccgatcga cgcgttcaac gcgccgttcc tgcagatccg ctggaaacgc
     4561 agcggtgagc cgccccacca ccggcggccc tacgttgaat ggctgcgcga tgaggaccag
     4621 gaatggagcc cgcagcggcg cgtccatatc tacttcgatc acgaagagca cgccgagggg
     4681 acggaccttg tcttctcaat gatcgagatg tatcgccatc cgctgtggcg cggtcagatc
     4741 aagcggctgc gtttctgcct ggcgccgggc gaaaccggcg tcgagttcga aattgattcg
     4801 atcttcaccg tctacgacac gcgccacacg atcaacaatc cgatcttcat cctggcctgc
     4861 tggaacacgt tccgctggac gggggatctg gccttcctgc gcgcgaacat caatcggatg
     4921 cggctggcgc tgcgctatca gcagtacgcg atgagcgcgc ttgagctggg acacatccgc
     4981 aacccgtggg tcggccacga cggtctggcc ggctggcaat tgggttcgga ggggggcaag
     5041 gatatccagt caggccatgg catcggcaac aattactggg acctgatgcc gttcggccgc
     5101 gacgacctgt atgccacgtc gcaataccat gcggcgacgc tggcgatggc cgaggtggag
     5161 gaggcggtgc tggatcaccc cggctgggat ctgccgcgcg gtccgctggg gctcgatccg
     5221 acgctgttgc gccggtatgc cgaagaagtg cgccagacgg ccaacgcgct gttctggaat
     5281 ccggagaccg gccggttcca cgccgccatc gacgcggagg gcaagccgca tgattacggc
     5341 ttcacctacc tgaacctgga ggcgatctgg tatggcctgg ccagcgaaga gcacgcggcg
     5401 cagatcatga gctggatcaa cggcgaccgg ttcgtcgtcg gcgatacctc gatggggaag
     5461 gacctctacg cctgggaatt cgcgccgcgc acgacgaccc gcagaaacct ggaatggtat
     5521 ggcccgacct ggaccaatcc ggaggaaatc gcctggggcg atcaggtgca ggacggcggc
     5581 gccgtgctgg gcttcagctt cttcgatctc tgggcgcggc tgcgcgtgct cgggcccgac
     5641 aacgcctgga accgcctcgg cgaaatcatc gactggcagg aacgcgtctg gcgccagggg
     5701 gattaccgcg attactacgc ggcggcgggt cgcggcaagc tgcagggctg cggcacgccc
     5761 ggcggcctgg gcatcgactg cgagttttat gaaagcagcc tgctgccggc gattgtggtg
     5821 tatgggtttc tggggcttga tccgcgcgct gattgtctgg cgatccaccc gcgcctcccc
     5881 ggcgcgacgc cggcaatgac cgtgcgcaac gtgctttacc actggacgcc gctggacatc
     5941 acggcgggcg gcgactcgat cgagatcacg ttgaaggaga cgccgctcga tccgattcag
     6001 atccaggtcc cggagggtta ccggctggcg gagagcgacg cggcgggccc gctctttcaa
     6061 atcgcgaagg cgggcgccta ccgcttcgag cgctagggca aggccggcga cagccgccct
     6121 gccggcgccg cgcggcgatc aggacgcctg cttcacccat ttgagcgcga gcttcctgcc
     6181 cgagcgggca cattcgcgaa ggtagaggtt gatcaggttc tggtagggaa cgccggtttc
     6241 ctgcgccatg gctttgaaat actcgatcac atcggtgcca agtcgaagtg tcacctgctt
     6301 gcggaaatgt atggcgtaag ggtttcgctt cgagttggtg aaatcgtatt cttttctcat
     6361 ggtagatcct cgtactgctt gcgctcaagc gacgtagcct tgcgtgccga gataattcgg
     6421 attagtccgg catcttctcg atagacatga caaacgacca atatccgcag gctcgtgctc
     6481 attcccagta tgagataccg atcttcatca ttggagtgat ccgggtcgta catcaatcgg
     6541 cctaattcgt cggcaaatac cgtggaggcc tcctcaaaag agactgcatg ctttctggag
     6601 ttcagttcgg ctttggcagg gtcccaggta aaccgtattg aactcatatt gatattgtat
     6661 ggctctaatc attatgactc aagaagaaat ggttcatccg cccgccccgc gtctgcttcc
     6721 tggaacgaga gcctgcaaag ccggcctttg aaataaaggt ttgttcgact gccctgcttc
     6781 agggagacga ttgagttctc acaagttcac tcccgccgaa agccatctcg gtgtctccac
     6841 ctttcccgcc cgcacgggag ggtgggttac atcgcgccgt agccggtcag gacgacgtag
     6901 accgtgcggg cgaggatttt gaaatcgagc agcagcgacc agttgtcgat gtactcaagg
     6961 tccagtttca tccaggtgtc gaaggggagc agattgcggc cgctgacctg ccagatgcag
     7021 gtcaggccgg gcttcatcga aaggcggcgg cgttgccagc ggtcgtattt ctcgatttcc
     7081 tgggggaggg gagggcgcgg cccgacgagc gacatttcgc cccgcacgac gttccacagt
     7141 tgcggcagtt catcgatcga gaacttgcgg aggaaccggc cgagcggcgt gatgcgcgga
     7201 tcgtgacgca tcttgaaaac gggcccctgt tgctcattga gctgctccag ggtttcccgt
     7261 tccatttcgg cgtccgagcg catcgagcgg aatttccagc aggtgaagag ccggccgttg
     7321 aggccgacgc gctgctggct gaagatgatc ggttcgccct tgcgcgaggt cagccggatg
     7381 gcgatggccg tcgccagcat cacgggcgac gccagcacga gcaggatcac ggcggcgatc
     7441 cggtcgaacg catatttgaa gaggagggcg ggcccgatcg gctgcgtcgg ccagtaggag
     7501 aggaccgggg tttcctcaaa gcggtcgatg acggggtggg cgatgcgggc ggagaagaaa
     7561 ttgagcggca ggtgcgtgcg gacgcccatc tcctcgcaga tctcgagcag cggccgcagg
     7621 ctggccagcg gggcggactc gggcaggaaa atgacttcgt cgatcggggt gcgccagagg
     7681 acggcggggg cgcgctccag gtcggcgagg attggccagc cgagcgtcga ttccgggacg
     7741 cgatcgggat cgaagccggc gccctggcag tcgggccggt cggtgacgat gccgacgatg
     7801 ttgagccccc agatcgggtg atgggcgatc gtttcggcga attcgctcag cgcggcgccc
     7861 gaaccgacga ggatcacgcg gcggtcgtgg aggccgcgcc ggcgcatcgc catcaggaag
     7921 cgccgcagca gccacgcctt gccggtcagc agcgcgaagg caaccagcgg gaaaaggacc
     7981 aggatcgcgc gcgaggtgtt ggccgcttcg gccgggcgca ggcgccagcc gaggaactcg
     8041 accccgccga acagaaagtc gatgcccacc aggatcagcg cggcctcgaa gcatgatgtg
     8101 gcgatgcggc gcgcctgttc ggccgtgcgg atccgcaggc gcagatcgcg ataggcgccg
     8161 ttgtaggcca gcaccagcac gatggccagc ggcccggccg gcaacagcca gaggtaatag
     8221 cccagcgtgg agggactgac gaagccgggg acgatgtaga tgctgagcag ggcgcgcagg
     8281 gcatgggcga tgaggaaggc cgcggcgccc agcagcaggt cgagcgcgat gttgacctga
     8341 cggaggatgt actggctttc acgcagcatg aggggagggg cgggtcgcgc gcgcgaccga
     8401 ctgaccgagt cgggacatcc gccggcgggc cggaaagcgg ccgccgggca cgccaccaat
     8461 gtatgccagc ggcggaaagt gtgtcaaacc gggcaagggg gggcggtact cgggaagtcc
     8521 gcaggcgttt acggttcgag gatggcgacc tcggcctgga acgagcggcc gccgacggcg
     8581 atcgtcatgc gcagcggtcg gccgttgtgg acggcggccg ccgccgcctg gggctggggc
     8641 gcggcgggcg ccgggggggg tgcgggggcg gccacgggcg cgggggacgg cgccggcgcc
     8701 gccggccgcg gattgatctt ctgttcgtaa aagtccagcc acaccttggg gaagatcgcg
     8761 taggagagga catcctcgtc gttgcgcgct ttgccctcca cctcctggcg gatcttgtcc
     8821 cactcgttgg ggatgagatc ggccgggcgg acggtgatcg gctgttcgtc gccgaggacc
     8881 tttttctgga tttcggggtc gaccggggcc ggcgtctccc catagcgtcc ggcgaaaata
     8941 ttgcggctct cctcggggac ggtctgatag cgattgccgg tcaggacgtt catcactgcc
     9001 tgcgtgccga cgatctgcga ggtgggggtc accagcggga tgtagcccat ctccttgcgg
     9061 acgcgcggca cttcctccag cacctcgtcg atccggtcga agcagttcat tttcttgagc
     9121 tggctttcga ggttggagag catgccgccg gggatctggc tgacgaagat gcgcgggtcg
     9181 gcgccggtga acgtgctttc aaattcgctg tatttggggc ggatcttgcg cacgtagtcg
     9241 gcgatcggca gcaggtcctc ctgtttcagc cccgtgtcgt gaggggtcgt gcggagcgtt
     9301 tcgacgatcg cttcggtggg gacatggccc gaaccgagcg agagcgcgct gatggcggta
     9361 tcgacgccgt cggcgcccgc ctcgatggcc cgcagcagga ccatcggcgc cacgccggtt
     9421 gtcgcgtgcg tatgaacatg gacggggatt ttgaccgcct ccttgagcgc gcgaaccagc
     9481 tcgtaggccg gcacgggggt catcagcgcg gccatgtcct tgacgacgag cgactggcag
     9541 cccagatcct cgatctgacg ggcggtctgg acgaacttct ccagcgtgtg gacgggcgag
     9601 accgtgtagc agatggcgcc ctgggcgtgg gcgcccccct tgagcacggc tcggaccgcc
     9661 gtcttcagat tgcgcacgtc gttcagggcg tcgaagatgc gaaagacgtc catcccgttg
     9721 gcgatcgagc gatcgacgaa ccgttcgacg atatcgtcgg cgtaattgcg gtagcccagg
     9781 atattctggc cgcgcaggag catctgcagc ggcgtccgcg gcaggtgctt tttgagcgtg
     9841 cgcaggcgct cccagggatc ctcgcccagg aaccgcaggc aggaatcgaa cgtggcgccg
     9901 ccccagacct cgagcgacca gtagccgacc ttgtcgagct gttcgcagat cggcaccatg
     9961 tcctcggtgc gcatgcgggt cgccagcagc gattgatgcg cgtcgcgcag cagcagttcg
    10021 gtgatccgaa tcggtttggg tgcgttggta tcgtcggcgc ctgccatggg tgtctcgctt
    10081 tgttctcggt ctggtgggcg ttccccccga accgccagca taacagggcg cgggggagga
    10141 agccagtcag agtcgcgtgc gaacgacgcg ccggccgacg ctttcgccgc ggatcatcgc
    10201 ctcgagcgcg gggatcgcct cctccagcgg gatctcggtg gcgagggcgt ccaggctggg
    10261 gatcttccac ggcccggcca gccggcgcca gacctcgcgg cgcatcggat tggggcactc
    10321 ggccgagtcg atgccgagca ggttgacccc gcgcaggatg aagggaaaga ccgagacgtg
    10381 cagttcgggg ccggcgacca tgccgcaggt ggtgacggcg ccgcgccggt gggtggagcg
    10441 cagcaggatc gaaagggctt caccgccgac gcaatcgacg gcgccggccc agcgcgcgct
    10501 gagcagcggc cgggcggcat cggtcatgaa ttcctcgcgg ccgaggactt cggcggcgcc
    10561 gatctcgcgc agccacggct cgcgcgcgcg cttgccggtg atggcggcga cgcgatagcc
    10621 cagatgggcc agcagggcga cggcgaagct gccgacgccg ccggtggcgc cggtgacggc
    10681 caccgcgccc gagccgggca gcacgccgtg ttcctccagg cgccagaccg attgcgccgc
    10741 cgtgaagccg gccgtgccga tgatcattgc gtcgcgcagc gtcaggccgt cgggcaacgg
    10801 cacgacccag tcggcgggca cgcggatcat cccggccaag ccccccgcgg tattcatgcc
    10861 caggtcgcgg ccggtgacga tgaccgggtc gccctcgcgg aaggcgccgt tgcggctcgc
    10921 cacgacgacg ccggcggcat cgatgcccgg tgtatgcggg taggcgcgcg tcacgccgcg
    10981 gttgccgtgc gccgacagcg catccttgta gttgagcgag gagtagtgca cctcgatcag
    11041 gacgtcgcca tcgggcaggt cgtcgctgtc gcgctcgacg atgcgccgga tgaaaacgcc
    11101 gccagtctgt tcttccaccc acagggcttt aaacttcggc acgatcgact ccagtgcttg
    11161 ggggatgcgg tgatgcgagg cgaacgtcag tccaccattt cgacggtgac ccagcggcgg
    11221 ccccagtcgt tggccttgcc gtgcgagcga tgaaagagat cgatgcggtc gggaccctgg
    11281 atggccgagc cgcggtcctc gacgcggccc cagccccagc cggggatgac catttcggtg
    11341 ccaaagggat agtagtcggt gtcggcggcg atcgtcccgg gctgccggag gattagccac
    11401 ggcaatagca ggcgcggcgc cgccatccac ggatgcacca gcgtgttcca ggagaggagg
    11461 cccggccgcg ggggatgcgg gcgcatgccg ttggccgtcc ggccggtgta tttctggccc
    11521 ttgccccagt cggcggtgac gtagcggttc cagaagtcga gtttgaggaa tttccaactg
    11581 ccgcgcgtgt agccgttgca ctcgccgcag ccgcagtagg cggtcgccag catctggcgc
    11641 gtctgaggac gggcgcagcc agccatgagg catgccgttc ccaacaacac tacgcagagg
    11701 cgggagcgga aacgaccgtg agacaggcga gccggggacg accgaagcat ggcatcgagc
    11761 ctcggggcca gtttaaggtg ctggcctggg atggtcaagc gggggagcgc cggcggcggc
    11821 ggcgcgccgt gagcttcgga tgggacaggt ccgatgttgc g
//
LOCUS       DAOPCO010000104        11799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5359_length_11799_cov_4.861546, whole genome shotgun sequence.
ACCESSION   DAOPCO010000104 DAOPCO010000000
VERSION     DAOPCO010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11799
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_11799_cov_4.861546"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PLS90_08760"
     CDS             complement(<1..475)
                     /locus_tag="PLS90_08760"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HPK02535.1"
                     /translation="MESTALFLTGTAVGCLIGVIGARFYQKRATVNRIQSAREEARLI
                     LDSARAEADKSLQLAEQRAKEEAQRRVKQVERELAKRRHEAERRERKLAQREKALERR
                     EETIAGQEAELERGRHQCAAREAALARQTAEVEAQRAEAIRRCEEISGLTSDQARK"
     gene            728..1963
                     /locus_tag="PLS90_08765"
     CDS             728..1963
                     /locus_tag="PLS90_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPK02536.1"
                     /translation="MSGLAQIPPAPSVRPLQALLIKPAGPDCNLACRYCFYLQKAALF
                     PDSKRHRISNPVLEATIRQALESGSPMVNFGWQGGEPTLMGLDFFRCAVQLQQSLSFP
                     GQQVANGLQTNGLLLDENWAAFLRDHRFLVGLSLDGPAHIHNHYRRTAGDADCYPRVI
                     RAARRLRTAGVQTNALTVIHDYSVRFPEEIYDFHRSEGLRWMQFIPCLEPDPAAPGRP
                     AAFSAPPRAYGEFLCRVFDRWIADFRDGWPRTSVRWFDSLFHTYVGQPAPECTLLPEC
                     GVYVVVEHNGDVYCCDFYVESRWRLGNVLEGDLLDMLNSTRQREFGRIKAALPRECAD
                     CPWLDHCRGGCPKDRQFTPSAGGTNYFCESYRLFFEHADARLRRLAADWRRRQAASPV
                     GGATTTSPARPRRKRVHRR"
     gene            complement(2532..3464)
                     /locus_tag="PLS90_08770"
     CDS             complement(2532..3464)
                     /locus_tag="PLS90_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HPK02537.1"
                     /translation="MDPTVKQLRYLVAVADQLHFRRAAEACHVTQPALSEQIAQLEDL
                     LGVRLVERSRRRVMMTPVGEEVVRRARRVLRDLDDLVQTAGRAGQPFSTPLRMGVIPT
                     IAPYLLPRILAQVRAESPELQLLLREEQTGRVVADLREGRLDVGLLALPIEGDDLEVQ
                     ELYQEDFVLLSPRGRGRQSTRRLPVTELAQRRVLLLEEGHCLRQQALEVCAMAGAREN
                     DDFRASSLGTLVQMVANGIGVTLLPWLALDVEVRGQSALAVRRFKPPVPSRRVGLIWR
                     RHSARRDEIRDLGRMIHRHLPAADRNGFRPAPAA"
     gene            3713..4249
                     /locus_tag="PLS90_08775"
     CDS             3713..4249
                     /locus_tag="PLS90_08775"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPK02538.1"
                     /translation="MLTIGDRFPSFSLKGVYTDDPKTAFKDVTDKDDAGKWKIFFFWP
                     KDFTFVCPTEIVAFGQMNSDFNDRDAVVYGVSTDTEFVHLAWRQNHPDLKGIPFAMLA
                     DIKRELSHALGVLDAKEGVCLRATFIVDPDNIIRFVSVNDLSVGRNPKEVLRVLDALQ
                     TDELCPCNWEKGQPTLTV"
     gene            4433..4981
                     /locus_tag="PLS90_08780"
     CDS             4433..4981
                     /locus_tag="PLS90_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPK02539.1"
                     /translation="MSIDRLREMLPEYAKDLRLNLSSLIANPEGMTDQQLWGTFVASA
                     CAAGEPTVIAAAIEQARAHISDEAVTAAKAAAAIMSMNNVYYRFVHLAGNKDYSQMPA
                     KLRMNVIANPGVDKQDFELWSLAVSAINGCGMCIDSHERQLVKAGLAKETIQHAVRIA
                     AVVRAVAATLAAEAAAQSYTAN"
     gene            5095..5183
                     /locus_tag="PLS90_08785"
     tRNA            5095..5183
                     /locus_tag="PLS90_08785"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5129..5131,aa:Ser,seq:gga)
     gene            5406..6443
                     /locus_tag="PLS90_08790"
     CDS             5406..6443
                     /locus_tag="PLS90_08790"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPK02540.1"
                     /translation="MSIVQEPFGRIDGQAVSLFTLTNAHGLRARITNYGCTIVALETP
                     DRDGRLADIVLGFDDVARYPGGPYFGAIIGRFANRIAGACFTLAGREYRLAANNGPNH
                     VHGGIKGFDKRVWTPRIIPRDGREALQLAYLSPDGEEGYPGNLDCAVIYDLNDQDEFV
                     LEYSATTDRPTHVNLTNHSYFNLAGAGRGDILGHELTLGADAYTPSNAELIPTGEIRP
                     VDGTPFDFREPRAIGERIDQLGGGYDHNYCLRGGGGERPAFCARLREPAGGRILEVYT
                     TTPGVQFYTCSFADAPIIGKAGRAYGRHAAVCLETQHYPDAPNRPHFPSTELGAGQTY
                     RQVTIWRFCHE"
     gene            6539..6763
                     /locus_tag="PLS90_08795"
     CDS             6539..6763
                     /locus_tag="PLS90_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02541.1"
                     /translation="MKPHSLPMLGLCAVSLALLAACGPTPTGDAPAAEEAAPAAQAPP
                     RARSSFTRRSISSTPNRFWRRLRSSPGSTC"
     gene            6757..7020
                     /locus_tag="PLS90_08800"
     CDS             6757..7020
                     /locus_tag="PLS90_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02542.1"
                     /translation="MLSVYDSEAAKTTGLVARLVAERDRPRCDVFWNNEIVRTIQLQH
                     EGLLQPYAAPGAAHVPAAFKDPEGYWTGFAGRMRVVAFNTDRY"
     gene            7033..7677
                     /locus_tag="PLS90_08805"
     CDS             7033..7677
                     /locus_tag="PLS90_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK02543.1"
                     /translation="MGDAAARPAPPASLTQLAAPEFKGQVAMAYPLFGTTATHGAILF
                     ACWGPAEACAFFERLAANDMRIMEGNMTAAKAVAAGEIPLCTTDSDDACLLRGEGQPI
                     GWLPIHHEPAAADRGGALMIPNTLALMAGAPHPEEGRALIDYLLAPTVEARLANSPSR
                     QIPLLRAADGFTTAALPADVRAMMAEPLIDPQFHVAADYLEGSAEFFRATFARP"
     gene            complement(7992..8591)
                     /locus_tag="PLS90_08810"
     CDS             complement(7992..8591)
                     /locus_tag="PLS90_08810"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HPK02544.1"
                     /translation="MTHPDRQEEKKALRRQARAARAALDPGWRAEASRRMHEHLLRID
                     PVREAETISLFVSVGAEVDTHPLIHAFLAAHKRVAVPCVLHAQRRLELRVIESFPDGF
                     QARDFGILEPEPLHHPEVVGIADVDVILVPGLLFNPGNGIRLGYGGGYYDRLLAEEHQ
                     ALAIALGFSTQLVAELPVEPWDRPVDAICTDAGWHPIRI"
     gene            complement(8617..9381)
                     /locus_tag="PLS90_08815"
     CDS             complement(8617..9381)
                     /locus_tag="PLS90_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HPK02545.1"
                     /translation="MPDALTHRRVPPALIAAAGGGKRLLPMTADRPKCMLEVGGEPIL
                     IHQIRALQACGVSQIQLIVGHGEESVRAACAALDHDGISFARNERHATTNSLFSFGCT
                     TLDPYPDGLLVLNSDVLFHPTLLRRLIDNPRENVLLADLRSRLGEEEMKICLDTDFRI
                     RRISKLVDPLQAQAENLGVLKLGPETASRMLRLARADGADTRLCWIPDAIDFLAGEID
                     FFALGSGELPWIEIDYPHDLRRAREEIWPQIQQGSI"
     gene            complement(9473..10585)
                     /locus_tag="PLS90_08820"
     CDS             complement(9473..10585)
                     /locus_tag="PLS90_08820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPK02546.1"
                     /translation="MAAPSVSIIIPCHGHAHWLPEAVRSALEQTHPSVEVIIVNDGSP
                     DDTARVGRAWAERHPDRVQYREQPNQGQARARQTGLEAARGEFVLCLDADDRLAPEMV
                     SICLGEFGRRPAAAAVVANAWLVAADGRSRLRPFDQRQRVRWPAVLASNPVGMFGAAL
                     LRTETVRALGGVAVAGACGAEDWDLWVRVARCGLAVAHRPERLACYRQSGASHSRRAL
                     ETLRAYGELLDRAMRPDERLSGWPRVAGPLAAEAAAGLMNERVFYALGVALAGSAGAD
                     ELASLMAFLRPGALSVRRANDQLLSALQFSLAAAGRRELRPTVPVDQIVALVREAASA
                     RGLAPGGALEGQLRRTLANPWRRRSLAARLRGRLRL"
     gene            10848..11168
                     /locus_tag="PLS90_08825"
     CDS             10848..11168
                     /locus_tag="PLS90_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02547.1"
                     /translation="MRAIPTPEIVDFGPKAIQAGEPVTVCDGHCAMWLSTVNATPETI
                     VVMRNRVLESSYAGPKKMSALIPDEFIDHPGFYEFYLLDRGTHKKSETRVLIVEGAPA
                     LASV"
     gene            11285..11578
                     /locus_tag="PLS90_08830"
     CDS             11285..11578
                     /locus_tag="PLS90_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HPK02548.1"
                     /translation="MPPLNQLFDPTFLAIWAAFGCVVGIVARKIMFPRRRGGGGFGGL
                     IQTSILGMLGSLVGVAAGVLLRLPGAGELGLISLVLAVVGTLLVVIIVTKFQG"
     gene            complement(11553..>11799)
                     /locus_tag="PLS90_08835"
     CDS             complement(11553..>11799)
                     /locus_tag="PLS90_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017842612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HPK02549.1"
                     /translation="LEYLLGPRSVIPYRAVFVAFTWVGAVLPIGLIIDFSDVMNGLMA
                     IPNLIGLVLLAGVVNRETNSYFAKRKAGAVSPETSSQ"
BASE COUNT         1882 a         4303 c         3721 g         1893 t
ORIGIN      
        1 gcttgcgggc ctggtcgctg gtcaggcccg aaatctcctc gcagcggcgg atcgcctcgg
       61 cgcgttgcgc ctcgacctcc gccgtctgcc gcgccagcgc agcttcgcgg gcggcgcact
      121 ggtggcgtcc ccgttccagc tccgcttcct gtcctgcaat cgtctcctcc ctgcgttcga
      181 gcgccttttc gcgctgcgcc agcttccgct cgcgccgctc ggcctcgtgg cgccgcttgg
      241 ccagttcccg ttcgacctgc ttgacgcgcc gctgggcctc ttccttggct cgctgttcgg
      301 caagttggag cgatttgtcg gcttccgccc gcgccgaatc caggattaac cgggcttcct
      361 cacgggcgga ttggatgcga ttaacggtcg cccgcttctg gtaaaaccgg gcgccaatga
      421 ctccgatcag gcaacccaca gcggtcccgg tcaggaacaa cgctgtggat tccatcaggt
      481 aatctttcaa cctcctgtgg atacgggttg gtgtcagggc agatcaacat cgtccgccgg
      541 cgccccgtcc cagggaccga accccggcgg caagacctcc caatgaggaa agtctcccca
      601 atgttagaat tcgacgcaag cgcccgtttg tcaaggtgat ttcctgatcg gcgcatcggc
      661 tgttccccca ccgccgcgcc gccagaaacc cctcgccaaa tcgacttgcc cctccttaat
      721 atgccccatg tccggtttgg ctcaaatccc cccggccccc tccgtccgcc cgctccaggc
      781 cttgctcatc aagccggccg gacccgactg caatctggcc tgccgctatt gtttctacct
      841 gcaaaaggcc gcgcttttcc cggattccaa gcgccaccgc ataagcaacc ccgtgctgga
      901 agcaaccatc cgccaggcac tcgaatccgg ctcgccgatg gtcaactttg gctggcaggg
      961 gggcgagccg acgctgatgg gtctggactt tttccgctgc gccgtccagc tccagcaatc
     1021 gctcagcttc cccggtcagc aggtcgccaa tggactgcag accaatggcc tcctgctcga
     1081 cgagaactgg gccgcgttcc tccgcgacca tcggttcctt gtcggcctct ccctcgatgg
     1141 ccccgcccac atccacaatc attaccgccg cacggcgggc gacgccgatt gctatccgcg
     1201 cgtgatccgc gccgcgcggc ggctgcgcac ggccggtgtg cagaccaatg ccctgaccgt
     1261 catccatgac tattccgtcc gcttccccga ggagatctac gatttccacc ggagcgaggg
     1321 cctgcgctgg atgcagttta tcccgtgcct cgagccggat ccggccgcgc ccggacgccc
     1381 cgccgcgttc tccgccccgc cgcgggccta cggcgagttc ctctgccggg tcttcgaccg
     1441 ctggattgcg gacttccgcg acggatggcc ccggacctcc gtccgctggt tcgattcgct
     1501 ctttcacacc tacgtcggcc agcccgcccc cgaatgcacc ctcctgcctg agtgcggggt
     1561 ttacgtcgtc gtcgagcata acggcgacgt ctattgttgc gatttctacg tcgagtcgcg
     1621 atggcggctc ggcaatgtgc tggagggcga cctgctcgac atgctgaatt cgacgcgcca
     1681 gcgcgagttc ggccggatca aggccgcgct cccccgcgaa tgcgcggatt gcccctggct
     1741 cgaccattgc cgcggcggtt gtcccaagga ccggcagttc accccctccg ccggcggtac
     1801 aaattacttc tgcgagtcgt atcgcctgtt cttcgaacat gccgacgcgc ggctgcggcg
     1861 gctggcggcg gactggcgcc gacgccaggc tgcgagcccg gtcggcggcg cgacaaccac
     1921 gagccccgcc cgaccccgtc gcaaacgcgt tcaccgccgc tgaccggcac cgcgcgcggc
     1981 ttggcattgc ccccaattgt tatcactcat cgttatcact catcgtaatc ttccctgttc
     2041 ataaccatcc cctcccaccc cggattcatc ctccgcgcct gcacccctcc gcgtctcgtc
     2101 ggctcggcgt gagaccatca tccccaacgg ccctccccca caatccggct cccggctctc
     2161 gcccatcgtc cccggctccc gaaaagacct tacaagatcc acgaccgggt gtcggaccgc
     2221 cgccctcgcc ggtccgcgat ggcgaaactt gccgaacgac atggccgaac aacatgcagg
     2281 acgcattccg gctccggcca atcaggaacc gcgccagcgg ttcgatatcc aagccgtggg
     2341 ttgcatggcc gccgggcggc catgcaaccc acggtcagcg cggtcaacaa ccccgaaccg
     2401 attgatcggt tcgataattc aattctccgc tgcgaccgac cacacgcaga acgccaggca
     2461 agggcctcac gccgaaaccc cgagacgccg agatcaagag gtcggaaacg atttgccgcg
     2521 cgcggccggg ttcatgccgc cggggcgggg cggaagccgt tccggtcggc ggcgggcagg
     2581 tggcggtgga tcatccggcc caggtcgcgg atctcgtcgc gccgcgccga gtgccgccgc
     2641 cagatcagcc ccacccgccg cgaggggacc gggggcttga accgccgcac cgccagcgcg
     2701 ctctggccgc gcacttcgac atcgagcgcg agccacggca acagcgtcac gccgatgccg
     2761 ttcgccacca tctggacgag cgtccccagg ctgctggcgc ggaaatcgtc gttttcacgg
     2821 gcgccggcca tcgcgcagac ctccagcgcc tgttggcgca ggcagtgacc ttcctcgagc
     2881 agcagcacgc ggcgctgcgc cagctcggtc accggcaggc gccgcgtcga ctggcggccc
     2941 cgcccccgcg gcgacagcag gacgaaatcc tcctgataca gctcctggac ctccaggtcg
     3001 tcgccttcga tcggcagcgc gagcaggccg acgtcgagcc ggccctcgcg caggtcggcc
     3061 accacccggc cagtctgctc ctcgcgcagc agcagctgca actcgggaga ctccgcgcgc
     3121 acctgggcga ggatccgcgg cagcagatac ggtgcgatcg tcggaatgac gcccatgcgc
     3181 agcggcgtcg agaaaggctg gccggcgcgc cccgccgtct gcaccagatc gtccagatcg
     3241 cgcaggacgc ggcgcgcgcg ccggaccacc tcctcgccca ccggcgtcat catcacgcgg
     3301 cggcgactgc gctccaccag ccgcacgccg agcagatcct ccagttgggc gatctgctcg
     3361 ctcagcgccg gctgcgtcac gtgacacgcc tcggcggcgc ggcggaaatg caattggtcg
     3421 gccacggcga ccaggtagcg gagttgtttg acggtcgggt ccacggggtc cctcgctcaa
     3481 gaatcgtccc tcgtcctcgg ctctcgaaaa cccgccagct tgtcccctcg ttgtaaacgg
     3541 ccggttcgtg agcacctcgt attcgccccc gccccctcta ttgataagca ttccctatca
     3601 tattgcaagg aaaatacaat ttgactgatt gacgccgccc ggctatcgta atggccaagt
     3661 ctggaggcaa ctcagcctgc aatcccaatt atccgcgaaa ggatccgcca tcatgctcac
     3721 catcggcgat cgcttcccct cgttcagtct caagggcgtc tacaccgatg accccaagac
     3781 cgccttcaag gacgtgaccg acaaggatga cgccggcaag tggaagatct tcttcttctg
     3841 gcccaaggac ttcacgttcg tctgccccac cgaaatcgtc gccttcggcc agatgaacag
     3901 cgatttcaac gaccgcgatg ccgtcgtcta cggcgtcagc accgacaccg agttcgttca
     3961 cctggcctgg cggcagaacc accccgacct gaagggcatc ccgttcgcca tgctggccga
     4021 catcaagcgc gagctctccc acgcgctcgg cgtgctcgac gccaaggagg gggtctgcct
     4081 gcgcgcgacg ttcatcgtcg accccgacaa catcatccgc ttcgtttcgg tgaacgacct
     4141 gagcgtgggc cgcaacccga aggaagtgct gcgcgtgctc gacgcgctgc agaccgacga
     4201 gctctgcccc tgcaactggg aaaagggcca gccgacgctc acggtctgag ccagcccgcc
     4261 gcgcgacggc cggcggcgcc cgggcccccc gcgccatgga ccgccggccg cagctcgcgg
     4321 ccagatttga gatgctcggg tcctgtatgc cgccccagcc cgccccgcgt taccggcctc
     4381 cgggaccaat cacccccctt ggaaccccat tgcccacctg gaggatgtca tcatgtcgat
     4441 cgaccgcttg cgcgagatgc tgcccgaata cgccaaggat ctgcgcctga acctgtcgag
     4501 tctgatcgcg aatcccgagg ggatgaccga ccagcaactg tgggggacgt tcgtcgcctc
     4561 ggcctgcgcc gcgggcgagc ccaccgtgat cgccgccgcc atcgaacagg cccgcgccca
     4621 catcagcgac gaggcggtca ccgccgccaa ggccgccgcc gcgatcatga gcatgaacaa
     4681 cgtctactac cgcttcgtcc atctggccgg caacaaggat tacagccaga tgccggccaa
     4741 gctgcggatg aacgtgatcg ccaaccccgg cgtcgacaag caggacttcg aactgtggtc
     4801 gctggcagtt tcggcgatca acggctgcgg gatgtgcatc gactcgcatg aacgccagtt
     4861 ggtcaaggcc ggcctcgcga aggagaccat ccagcacgcc gtccggatcg cggcggtggt
     4921 gcgcgccgtc gcggccaccc tggccgcgga agccgccgcg caatcctata cggcgaactg
     4981 agcgaccggg tgagacggtc ttcgggaaaa ggcttgtttc acccggccgc ttccctgtag
     5041 taatatcgcc ctgaccgcgc ggcgcctcgt tcgccgcgcg gccctgccca gggcggagag
     5101 gtgaccgagc ggtcgaaggt gcacgcctgg agagcgtgtg taccccttgg gtaccgaggg
     5161 ttcgaatccc tccctctccg ccatcccgga ttctgcccgc cccattcccg caaccagggc
     5221 tcggccctcc ccccggaccc cggatttcgg tccgcggcgc tcccgcgcgc acgtagggaa
     5281 atgcgcgcgc tatccccgat ccgcgcctgc gccgacgccg ccctcccgtg cgcgcaaatg
     5341 gggctttgcc cttcacccgc cgattttcca taatgcggcc agatcgcgcc cccgggagtc
     5401 cacggatgag cattgtgcag gaaccgttcg gccgcatcga cggccaggcc gtcagtctgt
     5461 tcacgctgac caacgcccac ggtctgcgcg cgcgcatcac gaactacggc tgcacgatcg
     5521 tcgcgctcga gacgcccgac cgcgacggac gcctggcgga catcgtgctg gggttcgacg
     5581 acgtcgcgcg ctaccccggc gggccgtatt tcggcgcgat catcggccgc ttcgccaacc
     5641 ggatcgccgg cgcgtgcttc acgctcgccg gccgcgagta tcggctcgcc gccaacaacg
     5701 gccccaacca cgttcacggc ggcatcaagg gcttcgacaa gcgcgtctgg accccgcgga
     5761 tcatcccgcg cgacggccgc gaggcgctcc agctcgccta cctcagcccc gacggcgagg
     5821 agggctatcc cggcaatctg gattgcgccg taatctatga tctgaacgac caggatgaat
     5881 tcgtgctgga gtacagcgcg acgaccgacc ggcccacgca cgtcaatctg accaaccata
     5941 gttattttaa tctggcgggc gcaggtcgcg gcgatatcct cggccacgaa ctgacgctcg
     6001 gcgccgacgc ctacaccccg tcgaacgctg aactgatccc gaccggcgag atccgcccgg
     6061 tcgacggcac gccgttcgat ttccgcgagc cgcgtgcgat cggcgagcgc atcgaccagc
     6121 tcggcggcgg ctacgatcac aattactgcc tgcgcggcgg cgggggcgag cggcccgcct
     6181 tctgcgcgcg gttgcgcgag cccgccggcg ggcgcattct ggaggtctac accaccaccc
     6241 ccggcgttca gttttatacc tgcagctttg ccgacgcccc gatcatcggc aaggccggcc
     6301 gcgcctacgg gcgccacgcc gccgtctgcc tcgaaaccca gcattacccc gacgcgccca
     6361 accgcccgca tttcccctcg accgaactcg gcgcgggcca gacctaccgt caggtgacga
     6421 tctggcgctt ttgccacgaa tgaccgccgg tggtggtttc cgcgcgcgcc cgcggcctgc
     6481 cgcctgctcg tccacgtccg cgtgcacgag gtgatttttt tcggattgga gccctcgcat
     6541 gaagccgcac tcgctgccga tgctcggcct ctgcgccgtc agtctcgcgc tgctggcggc
     6601 ctgcggcccc acgcccaccg gcgacgcgcc cgccgcggaa gaggccgcgc ccgccgcgca
     6661 ggcgccgccg cgcgcacggt cgtcgtttac acggcgctcg atcagctcta ctccgaaccg
     6721 attctggcgg cgtttgcgga gcagcccggg gtcgacgtgc tgagcgtcta cgattcggag
     6781 gcggccaaga caaccggcct ggtggcgcgc ctcgtcgccg agcgcgaccg cccgcgctgc
     6841 gacgtcttct ggaataacga aatcgtccgc acgatccagc tccagcacga gggtctcttg
     6901 cagccctacg ccgcgccggg cgccgctcac gtcccggcgg cgttcaagga tcccgaaggc
     6961 tattggaccg gctttgccgg ccgcatgcgc gtcgtcgcgt tcaacaccga ccgctactag
     7021 gcgccggctc cagtgggcga cgccgccgcg cggcccgcgc cccccgcgtc gctgacgcag
     7081 cttgccgcgc ccgaattcaa gggccaggtc gcgatggcgt atcccctctt tggcacgacg
     7141 gccacgcacg gcgcgatcct gttcgcatgc tgggggccgg ccgaggcatg cgcgttcttc
     7201 gagcgactgg ccgccaacga catgcggatc atggagggca acatgaccgc cgccaaagcc
     7261 gtcgctgccg gcgagatccc cctctgcacg accgacagcg acgacgcctg tctgctgcgc
     7321 ggcgagggcc aacccatcgg ctggctgccg atccaccacg aaccggccgc cgccgaccgg
     7381 gggggcgccc tgatgatccc caatacgctg gcgctgatgg ctggcgcgcc ccacccggag
     7441 gaaggccggg cgctgatcga ttacctgctg gcgccgaccg tcgaggcgcg gctggccaac
     7501 tcgccctcgc ggcagatccc gctgctgcgc gccgccgacg gcttcaccac cgccgcgctg
     7561 cccgccgacg tgcgcgcgat gatggccgag ccgctgatcg atccgcaatt ccacgtcgcg
     7621 gcggactatt tggaagggag cgccgagttt ttccgcgcga cgttcgcccg accgtaaaca
     7681 gcaacgtaag accgagcacg aagacgtgtt gcccatcaat ctgcggatca caacgcggga
     7741 cgagaatgga gggtgaaccg ccgattcgaa cgacccaggc cttgccggat cgatcagccg
     7801 gccagtcccg tcagggacgg cagaaccaac ttcaagcaat cccgagaata ggggcagatt
     7861 ttcgagtgag cctttcacga cgccttcggc gccgccctga atgaatgaac ttgccctgct
     7921 tgcgcccctc ctgtccctta cgtcccctct gtcccttatg tcctttacgc cccttctgaa
     7981 accctcacgg ctcagatccg gatcgggtgc cagcccgcgt cggtgcagat cgcatcgacc
     8041 ggcctgtccc acggctcaac cggcagctcc gcgaccaact gggtcgagaa ccccagcgcg
     8101 attgcgagcg cctggtgctc ctcggccagc agccggtcgt aatagccccc gccgtagccc
     8161 agccggatgc cgttgcccgg gttgaacagc agccccggca cgagaatcac atcgacatcg
     8221 gcgatcccga cgacctcggg gtgatgcagc ggttcgggtt cgaggatgcc gaaatcgcgc
     8281 gcctgaaagc cgtcgggaaa cgactcgatg acgcgcaact cgaggcggcg ctgggcatgc
     8341 agcacgcagg ggaccgccac ccgtttgtgc gcggccagaa aggcgtggat cagcgggtgc
     8401 gtatcgacct cggcgccgac gctgacgaac agcgaaatcg tttcagcttc acgaaccggg
     8461 tcgatgcgga gcaggtgttc gtgcattcgg cgcgaggctt cggcgcgcca gccggggtcg
     8521 agggccgccc gcgccgcgcg cgcctggcgc cgcagggcct tcttctcttc ctgccgatcg
     8581 gggtgggtca tcttcggtcc gtgttctctc gtcgggtcag atgcttccct gctggatttg
     8641 cggccagatt tcctcgcggg cgcggcgcag atcgtggggg tagtcgatct cgatccacgg
     8701 cagctcgccc gagccgagcg cgaagaagtc gatctcgccg gccaggaaat cgatcgcatc
     8761 ggggatccag cacagccgcg tatcggcccc gtccgcccgc gccagccgca gcatccggct
     8821 ggccgtctcg ggccccagct tgagcacgcc gaggttttcg gcctgcgcct gaagcggatc
     8881 gaccagcttg ctgatgcgcc ggatccggaa atccgtgtcc aggcagatct tcatttcctc
     8941 ctcgcccagc cggctgcgca ggtcggccag cagcacgttc tcgcgggggt tgtcgatcag
     9001 ccggcgcagc agcgtcgggt ggaacagcac gtcggagttg agaacgagca ggccgtcggg
     9061 ataggggtcg agcgtcgtgc agccgaagga gaacaggctg ttggtggtgg cgtggcgttc
     9121 gttgcgggca aacgagatgc cgtcgtggtc gagcgccgcg caggccgcgc gcacgctctc
     9181 ctcgccgtgg ccgacgatca attgaatttg cgagacgccg cacgcctgca atgcccggat
     9241 ctgatggatc aggatcggct cgccgccgac ctcgagcatg cacttggggc ggtcggcggt
     9301 catcggcagc aggcgcttgc cgccgccggc ggcggcgatc agcgcgggcg ggacgcggcg
     9361 gtgggtcagg gcgtcgggca tcggggcttc ttcacggggc gctcgggcgt cggccaaagc
     9421 ccatactctg gcagccccgc cgcgcaaaag caaccgcgcg atccaaccag ctttacaagc
     9481 gcaaccggcc gcggaggcgc gcagccagcg agcgccggcg ccaggggttc gccagcgtcc
     9541 ggcgcagttg gccttccagc gcgccgcccg gcgcaagccc ccgcgccgac gcggcctcgc
     9601 gcacaagcgc cacgatctga tcgaccggca cggtcggccg cagttcacgc cggccggctg
     9661 ccgccaggct gaattgcagc gcactcagga gctggtcgtt cgcccggcgg acggagagcg
     9721 cgcccggccg caggaacgcc atgagcgatg ccagttcgtc ggccccggcg ctccccgcca
     9781 gcgccacgcc caacgcataa aagacgcgct cgttcatcag gccggcggcc gcttcggccg
     9841 cgagcggccc ggcgacgcgc ggccagccgc tcagccgctc atccgggcgc atcgcccggt
     9901 ccagcagctc gccgtaagcg cgcagcgtct ccagcgcgcg gcggctgtgg ctcgcgccgc
     9961 tctggcgata gcaggccagc cgctcgggcc gatgcgcgac cgccaggccg caccgtgcga
    10021 cgcgtaccca caaatcccag tcctcggcgc cgcaggcccc cgccacggcg acgcccccca
    10081 gcgcccggac ggtctcggtc cgcagcaacg ccgcgccgaa catgccgacc gggttggacg
    10141 cgaggaccgc cggccagcgc acgcgctggc gctgatcgaa cggccgcagc cggctccgcc
    10201 cgtccgccgc cacgagccag gcgttcgcca cgacggccgc ggcggcgggg cgccggccga
    10261 actcgccgag gcagatcgag accatctccg gcgcgaggcg atcatcggcg tccagacaga
    10321 ggacaaactc gccgcgcgcg gcctccaggc cggtctggcg cgcgcgcgcc tgcccctggt
    10381 ttggctgctc ccgatactgc acgcgatccg gatgccgctc ggcccatgcc cggccgacgc
    10441 gcgcggtgtc gtcgggcgag ccgtcgttaa cgatgatcac ttcaacgctc gggtgggtct
    10501 gctccagcgc cgaccggaca gcctcgggca gccagtgcgc gtgcccatgg caggggatga
    10561 tgatgctgac gctgggcgcg gccatgccgt ggaagatagc cccggcggcg ggccgctcgc
    10621 aaggccgccg agacgcgggc aagaaaccga atgggcttta agtcaaaaga tgatgaaaaa
    10681 gtacttgata gctggagtcg gttgtcctga ttattatcac aggcttgccc gtatcaggat
    10741 gctgcggatc gggaacggcg cggccccgcg gtctgggggc gtcgcccagc ccatccaaaa
    10801 actgttctct ttgcgctgat tgcaggaatg agaaagggga gatcacgatg agggccattc
    10861 cgaccccgga gattgtggat ttcggtccga aggcgattca ggcgggcgag cccgtcaccg
    10921 tctgcgacgg ccattgcgcg atgtggttga gcacggtcaa tgccactccg gagacgatcg
    10981 tcgtcatgcg caaccgggtg ctggaatcgt cctacgcggg accgaagaag atgtccgcgc
    11041 tgattccgga cgagttcatt gaccaccccg ggttctatga gttctatctg ctggaccgcg
    11101 gcacgcacaa gaagagcgaa acgcgcgtgc tgatcgtcga gggggcaccg gcgctcgcgt
    11161 cggtctgagc cgggcgctcc gcccgcggct cgcgaaccgc ccggcgcgtg gcgcgcgtcg
    11221 ggcacgcggg ccgggaaagc ggagccgacc gtcgggcccg ctcccgcgcg ccctggggat
    11281 tgccatgccg ccgctcaatc agttgtttga tcccacgttc ctggcgatct gggccgcctt
    11341 cggctgcgtc gtcggtatcg tcgcccgcaa gatcatgttt ccgcgccgcc ggggcggcgg
    11401 tggctttggc gggctcattc agaccagcat cctcggcatg ctcggctcgc tggtgggggt
    11461 ggcggccggc gtcctgctgc gcctccccgg cgcgggcgag ctgggcctga tcagcctggt
    11521 tctggccgtc gtcggcacgc tcctcgtcgt aatcattgtg acgaagtttc agggctgacc
    11581 gcccccgcct tgcgcttggc gaagtagctg ttcgtctccc ggttcacgac gcccgccagc
    11641 agcaccagcc cgatcaggtt cggaattgcc atcaggccgt tcatcacgtc ggaaaagtcg
    11701 atgatcagcc cgatgggcag cacggcgccg acccacgtga acgccacgaa cacggcccga
    11761 taagggatga ccgagcgcgg accgagcagg tattcgagc
//
LOCUS       DAOPCO010000105        11797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5360_length_11797_cov_4.560722, whole genome shotgun sequence.
ACCESSION   DAOPCO010000105 DAOPCO010000000
VERSION     DAOPCO010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5360_length_11797_cov_4.560722"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLS90_08840"
     CDS             complement(<1..202)
                     /locus_tag="PLS90_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPK02550.1"
                     /translation="MARYTGPVDRLCRREGKNLHLKGERYHKGKCPWLKRPFPPGASA
                     QSRTKQSPYANQLREKQSLKRIY"
     gene            complement(283..744)
                     /gene="rplQ"
                     /locus_tag="PLS90_08845"
     CDS             complement(283..744)
                     /gene="rplQ"
                     /locus_tag="PLS90_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPK02551.1"
                     /translation="MRHRKIKSRLNRTTEHRAAMLRNLAVALVEHERIRTTKPKADAL
                     RPFVESLVTLAKEGSLASKRLAISRLQDRFAVSKLFDQIAPRSKDRPGGYLRIVKDGV
                     RMGDGAAMAFIEFVDAAPGGDETTERPRDRRKLLRQRMHQRRKEMARMRQA"
     gene            complement(793..1806)
                     /locus_tag="PLS90_08850"
     CDS             complement(793..1806)
                     /locus_tag="PLS90_08850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPK02552.1"
                     /translation="MNFKAMIKPRHLAVETDTLTPTFGRFVAEPFERGFGVTIGNSLR
                     RILLSSIPGAAVVSVRIEGVAHEFMVKEGLREDISDVILNLKGLHIRMSPLIPQTTVY
                     LEAHGPKQVKASDIELNADVEILNPDHVIANLDEGAVLQMEITIATGRGYVPASEFKK
                     DPDDIGLVAIDAVFSPIQNVQYQVESARVGQVTDFDRLVLEVHTDGTVSPEEAVSTAA
                     QILKNHLDIFIRPGEEVEVLQPVGEEAEAALPASDVEEKLDKSIEELELSVRSYNCLE
                     AAGIKTIRDLVQKSESEMLKYRNFGRKSLTEIKNILKEMGLGFNMRLDETGMPIMSTD
                     ENA"
     gene            complement(1914..2312)
                     /gene="rpsK"
                     /locus_tag="PLS90_08855"
     CDS             complement(1914..2312)
                     /gene="rpsK"
                     /locus_tag="PLS90_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPK02553.1"
                     /translation="MAKKEKSAAKIKRKVRKNVTVGVAHIHATFNNTIVTITDTAGDT
                     LSWASAGSSNFKGSRKSTPFAAQLAAENAARKAMDHGLRSVEVWVRGPGSGREAAIRS
                     LQASGLEIRIIRDVTPIPHNGCRPPKKRRV"
     gene            complement(2385..2771)
                     /gene="rpsM"
                     /locus_tag="PLS90_08860"
     CDS             complement(2385..2771)
                     /gene="rpsM"
                     /locus_tag="PLS90_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008859826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPK02554.1"
                     /translation="MARIAGIDLPRDKRVEIGLRYIYGIGPARSQNLLRQAQINPDTR
                     VRDLTEEEVGRLNNLLTNAEYKVEGDLRREVQQNIKRLMDIGCYRGTRHIRGLPVRGQ
                     RTRTNARTRKGPARAIAGKKKAPPKG"
     gene            complement(2827..2940)
                     /gene="rpmJ"
                     /locus_tag="PLS90_08865"
     CDS             complement(2827..2940)
                     /gene="rpmJ"
                     /locus_tag="PLS90_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPK02555.1"
                     /translation="MKVRASVKKICNKCKIIRRKGVVRVICENPKHKQRQG"
     gene            complement(2965..3183)
                     /gene="infA"
                     /locus_tag="PLS90_08870"
     CDS             complement(2965..3183)
                     /gene="infA"
                     /locus_tag="PLS90_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPK02556.1"
                     /translation="MAKEDAIEISGTVIEPLPNAMFRVELENGLKVLAHISGKMRMNF
                     IRILPGDKVTVELSPYDLTRGRITYRFK"
     gene            complement(3388..4143)
                     /gene="map"
                     /locus_tag="PLS90_08875"
     CDS             complement(3388..4143)
                     /gene="map"
                     /locus_tag="PLS90_08875"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPK02557.1"
                     /translation="MANRITIKSKDELNTMRRAGALLREVMEEVIGHVRPGVTTGELD
                     RVARHEIERRGAIPAFLGLYDFPGSLCISINEEIVHGIPGKRVLVEGDIASLDLGLIL
                     DGYYADMARTVPVGRADEVSNRLIQVTGEALEIGIEFLRPGLRMGDLSAAVQAHVERN
                     GFAVVRDYTGHGIGRRPHEDPKIPNYGEPGRGVRWQVGMVVCIEPMVNVGTYQTLLRE
                     DRWTVVTADGSRSAHMEDTIAVTEDGPWVLTAP"
     gene            complement(4181..4885)
                     /locus_tag="PLS90_08880"
     CDS             complement(4181..4885)
                     /locus_tag="PLS90_08880"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPK02558.1"
                     /translation="MPEGGPVPQRMVIGILGPPGCGKGTQSERIQRTFHFAHLSSGGI
                     LRDEIRQQTPLGRLAGELMSHGNLVPDPVINEVMVRRLKSTIDHGDGVLLDGYPRTVP
                     QLRHLIGKLEAWAVPLNAMLYLQIDDDSLVERLTGRRICGTCGAVYHLLDLPPTVPNR
                     CDKCNGELIIRPDDAVDAIRERMRVYHNETEPILAEMQKEHYFYRIPAEGNPETVFHH
                     IRQVLEPLMHGSPSTL"
     gene            complement(4941..6368)
                     /gene="secY"
                     /locus_tag="PLS90_08885"
     CDS             complement(4941..6368)
                     /gene="secY"
                     /locus_tag="PLS90_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPK02559.1"
                     /translation="MILGSLINIFRVPDLRRKVLITLGLILVYRIGAHVPTPFIDPVK
                     IREFFRGTFGSGGVFNVVDMFSGGAFSQMTVFALGIMPYISASIILQLLTAVWPRLEK
                     ISKEGEAGRKKITQWTRYGTVGLSLFQGFGIGIYLLNEQLSLIPDHPALFLFVTAMTM
                     CTGTCFIMWLGEKISQHGIGNGISIIITIGIIARYPIDLNLALMSIRNNSMTPIGLMM
                     VLALGVLVAALIVFGQQANRKVPVQHARRMVGRKMVQGGNTFIPLKLNTAGVIPVIFS
                     SAILTFPAMILPMFGGGAPGSIGFTISSWLEMNSIHNPYNAFNLTSGGIFSLLKMVNI
                     YTLAYIALTAFFCFFYTAITLNPVDLADNLKKSGAYIPGIKPGKHTADYIDHILVRIT
                     VVGAFFLIVVALVPDMLYVSFNIPFGVAQMAGGTGLIIVVGVVLDTMMQIESQLMMRH
                     YEGFGRSGGRVGSRSRRPVVRMRGA"
     gene            complement(6371..6814)
                     /gene="rplO"
                     /locus_tag="PLS90_08890"
     CDS             complement(6371..6814)
                     /gene="rplO"
                     /locus_tag="PLS90_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006364582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPK02560.1"
                     /translation="MKMHELPGDPGRKQQPKRVGRGEGSGQGKTAGKGHKGQQARSGS
                     GKGPAFEGGQMRLIRRLPKFGFNNDRFRVRRDEVDLRDLNGFEDGATVDHEAMLAAGI
                     VSRQARRIKVLANGKLEKKLTVRAHGFSSAARAAIEAAGGRCEEI"
     gene            complement(6820..7005)
                     /gene="rpmD"
                     /locus_tag="PLS90_08895"
     CDS             complement(6820..7005)
                     /gene="rpmD"
                     /locus_tag="PLS90_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019174653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPK02561.1"
                     /translation="MASKLKITWVKSAIGAKKQHQRTIRALGLHRLNQSVVKDASDPV
                     RGMVFAVNHLVRVEEVD"
     gene            complement(7404..7916)
                     /gene="rpsE"
                     /locus_tag="PLS90_08900"
                     /pseudo
     CDS             complement(7404..7916)
                     /gene="rpsE"
                     /locus_tag="PLS90_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(7997..8359)
                     /gene="rplR"
                     /locus_tag="PLS90_08905"
     CDS             complement(7997..8359)
                     /gene="rplR"
                     /locus_tag="PLS90_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPK02562.1"
                     /translation="MALTRTEALTRRHLRVRRKVAGSPERPRLCIHKSLKHIYAQVID
                     DSAGRTICFVTTNNKANKSEARNFSNRAWAEKLGALIAERARAQGVEQVVFDRGGYRY
                     HGVVKAFAEAARKSGLKF"
     gene            complement(8427..8963)
                     /gene="rplF"
                     /locus_tag="PLS90_08910"
     CDS             complement(8427..8963)
                     /gene="rplF"
                     /locus_tag="PLS90_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPK02563.1"
                     /translation="MSRIGKIPITLPRGAEARIDGNVLTVKGPKGSLSLDFHNHVKVR
                     LEDGALLVERHSDERQDRAYHGLYQRLIRNMVTGVAEGFAKQLELVGVGYRATMDGKT
                     LVMNLGFSHEVRFGPIDGVTLAVPDPTHVTVNGIDKQLVGQVAANIRRIRPPEPYKGK
                     GIRYSGEHIRRKVGKAGV"
     gene            complement(9003..9455)
                     /gene="rpsH"
                     /locus_tag="PLS90_08915"
     CDS             complement(9003..9455)
                     /gene="rpsH"
                     /locus_tag="PLS90_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPK02564.1"
                     /translation="MSVVTDPIADMLTRLRNAIHAKQDSVRMPASKMKIAIAKILREE
                     GYIKYFKVVRAGSVKRNPKAGDKRDAKPLSHNLLIVYLKFGPGGEKVINGLTRVSRPG
                     LRRYSGSHDIPKVLGGMGTTIVSTSKGVMTGREARRQNVGGEIICSIW"
     gene            complement(9487..9672)
                     /locus_tag="PLS90_08920"
     CDS             complement(9487..9672)
                     /locus_tag="PLS90_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002557082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPK02565.1"
                     /translation="MARTCLKAKALRKPKFKTRQHNRCPLCGRSRAFMRQFKMCRLCF
                     RKYASLGQIPGVTKSSW"
     assembly_gap    10007..10106
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10245..10817)
                     /gene="rplE"
                     /locus_tag="PLS90_08925"
     CDS             complement(10245..10817)
                     /gene="rplE"
                     /locus_tag="PLS90_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPK02566.1"
                     /translation="MSDKATQNGYRPRLLADYQERIRPSLMDELKLKNVMEVPRLLKV
                     VLNMGVGEGSRDEKILQAAEEDLALISGQKPRRTRAKISVAAFKLRAGMPVGCMVTLR
                     GPYMYEFLERLINVAIPRIRDFRGLPPRAFDGKGNYNFGIREHTIFTEIDHRQVQQTF
                     GMNITLVTNAKSDDHCRTLLRRFGLPLREN"
     gene            complement(10867..11196)
                     /gene="rplX"
                     /locus_tag="PLS90_08930"
     CDS             complement(10867..11196)
                     /gene="rplX"
                     /locus_tag="PLS90_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPK02567.1"
                     /translation="MALRIKRDDLVLVISGAFKGARGRVLAVDTARQRAIVEGVNRVK
                     KHQKARGQQDAGGIIEKEAPIHMSNLMLVETGGRGRAARFRVKTDEHGNKQRVLKLRG
                     ESKEVTV"
     gene            complement(11208..11576)
                     /gene="rplN"
                     /locus_tag="PLS90_08935"
     CDS             complement(11208..11576)
                     /gene="rplN"
                     /locus_tag="PLS90_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPK02568.1"
                     /translation="MIQEYTVLDVADNSGARKLRCFKVLGGSRRRYASIGDVIVCSVR
                     EAAPDSNIKKGQVVKAVIVRTVKEVGREDGSYIRFDSNAAVLINANMEPIGTRIFGAV
                     GRELRKRNFMKIVSLAPEVI"
     gene            complement(11644..>11797)
                     /locus_tag="PLS90_08940"
     CDS             complement(11644..>11797)
                     /locus_tag="PLS90_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPK02569.1"
                     /translation="YGKTMRQTKRLYAHDEENNCREGDTVRVIETRPLSRHKRWRVSA
                     ILERAK"
BASE COUNT         1736 a         3608 c         4031 g         2322 t
ORIGIN      
        1 cgtagatccg cttgaggctc tgcttttcgc gcaactgatt ggcgtagggc gactgtttgg
       61 tgcgcgactg agcggacgcg cccgggggga acggccgctt gagccacggg catttgccct
      121 tgtggtagcg ttcgccctta aggtggagat tcttcccctc ccggcggcaa agccggtcaa
      181 cggggccggt ataacgtgcc acgctctgat atcctcctgc cttcggtgaa aggcgttgcg
      241 tccttcgctg gctgagttgg tcgcgggccg gtcggcgcgg ggctaggcct ggcgcatccg
      301 ggccatttcc ttgcgccgct gatgcatccg ctggcgcaac agtttgcggc gatccctggg
      361 ccgctcggtc gtttcgtcgc cgcccggcgc ggcgtcgaca aactcgatga acgccattgc
      421 ggcgccgtcg cccatccgca cgccgtcctt gacgatgcgc aggtagccgc cggggcggtc
      481 cttggaacgg ggggcgatct ggtcgaacag cttgctgacg gcgaagcgat cctgcaggcg
      541 gctgatcgcc aggcgcttgg aggcgagcga gccttccttg gccagggtca ccaggctttc
      601 gacgaagggg cgcagcgcat cggccttggg tttggtcgtg cggatgcgtt catgttcgac
      661 cagcgccacg gcgaggttgc gcagcatcgc cgcgcggtgt tcggtggttc ggttcagtcg
      721 gctcttgatc ttgcggtgtc gcatcgtcgt tcagttcctg ggttggactt ggcccggcgc
      781 cgcagcggcc gatcaggcgt tctcgtcggt cgacatgatc ggcatgccgg tctcgtcgag
      841 gcgcatgttg aagccgaggc ccatctcctt gaggatgttc ttgatttcgg tgagcgattt
      901 gcggccgaag ttgcggtatt tgagcatttc gctttcggat ttctggacca ggtcgcgaat
      961 cgtcttgatc ccggccgctt cgagacagtt gtaggagcgg accgacagtt cgagttcctc
     1021 gatgctcttg tcgagcttct cttcgacatc ggaggcgggc agggcggcct cggcttcctc
     1081 gccgacgggc tgcaggactt cgacttcctc gcccgggcgg atgaagatat cgaggtggtt
     1141 cttgaggatc tgcgccgcgg tagagacggc ctcctcgggg ctgacggtgc cgtcggtgtg
     1201 gacttcgagg acaagccgat cgaagtcggt cacctggccg acacgggcgc tctcgacctg
     1261 atactggaca ttctggatcg gggagaaaac ggcgtcgatc gcgaccaggc cgatgtcgtc
     1321 gggatccttc ttgaactcgg aggcggggac gtaaccgcgg ccggtggcga tcgtgatctc
     1381 catctggagc acggcgccct catccaggtt ggcgatgacg tgatcggggt tgaggatttc
     1441 gacgtcggcg ttcagttcga tgtcggaggc cttgacctgc ttcgggccat gggcctccag
     1501 gtagacggtg gtctgcggga tcagggggga catccggatg tggagcccct tgaggttgag
     1561 gatcacatcg gagatatcct cgcgcaggcc ttccttgacc atgaattcgt gggccacccc
     1621 ttcgatccgg acggagacga cggccgcgcc ggggatcgag gagaggagga tccggcgaag
     1681 ggaattgccg atggtgacgc caaagccgcg ctcgaacggt tcggcgacga accggccgaa
     1741 agtcggcgtc agggtgtcgg tctccacggc cagatgtcgc ggcttgatca tcgctttgaa
     1801 attcatctcg tcgcatcgct ccttgtgtga cgactggggc gggctggatg tccgatccgg
     1861 ccgtccgccg gtgaggaaac tcgtcctgct caggcaggga ctccgtgggc cgatcagacc
     1921 cgtcgtttct tgggcggtcg gcagccgttg tgcggaatcg gggtcacgtc gcggatgatc
     1981 cggatctcca ggccgctggc ctgcagggac cggatggcgg cctcgcgccc cgagccgggt
     2041 ccgcgcaccc agacctcgac cgagcgcagg ccgtgatcca tcgccttgcg ggcggcgttt
     2101 tcggcggcca actgggcggc gaacggggtg ctcttgcgcg agcccttgaa attggacgac
     2161 ccggccgagg cccaggagag cgtgtcgccg gcggtgtcgg taatcgtcac gatcgtgttg
     2221 ttgaacgtcg cgtggatatg cgccacgccg accgtgacat tcttgcggac cttgcgtttg
     2281 atcttggccg ctgatttttc cttcttcgcc atgccgtctc atccgttctg gtgaatcatg
     2341 gggcgtgtcg ggggaggtgt tgcgcccccg cggaggttgc gggctcagcc cttgggcggc
     2401 gccttcttct tgccggcgat ggcccgggcc gggcccttgc gcgtgcgggc gttggtccgc
     2461 gtgcgctggc cgcggacggg caggccgcgg atgtggcgcg tgccacgata gcagccgatg
     2521 tccatcagcc gcttgatgtt ctgctgaact tcgcggcgca ggtcgccctc gaccttatac
     2581 tcggcgttgg tcagcaggtt gttgaggcgg ccgacctcct cctcggtcag gtcgcggacg
     2641 cgggtatcgg ggttgatctg cgcctggcgg agcaggttct gcgagcgggc ggggccgatg
     2701 ccgtagatat aacggagccc gatttcgacg cgcttgtcgc gcgggaggtc gatgccggca
     2761 atgcgtgcca cgggtgctca ctccattcaa gcggaattca aaattcaagg gttggcgata
     2821 acgggctcag ccctggcgct gtttgtgttt ggggttttcg cagatcacgc ggacgacgcc
     2881 cttgcgccga atgatcttgc atttattgca gattttcttg accgaagctc gtactttcat
     2941 ggtcgtcgcc cctcgccccg tgcattattt gaaccggtaa gtgatgcgcc cgcgggtcaa
     3001 atcgtagggc gacagttcga cggtaacctt gtcgcccggc agaatccgaa tgaaattcat
     3061 gcgcatcttg cccgaaatgt gggccaggac cttcaacccg ttttccagct caacgcggaa
     3121 catcgcattg ggaaggggtt cgatgaccgt cccactgatt tcgatggcat cttctttggc
     3181 cataagcgga tctggatcag gctcccgcgt gtgttgggtg gttctggccg tatgactgga
     3241 actcatggag tccaaacaca caccaaaact cagatcttat gattccagag aggtggaatc
     3301 aagccttttt tgggcggcgc ggcgtctccc gggggcaaat ccgcccgggg ccgtcgcccg
     3361 cacgcccgct cccctcaccc cgggtgatca gggggccgtc aggacccagg gtccgtcctc
     3421 cgtcacggcg atcgtgtcct ccatgtgggc cgaccggctg ccgtcggccg taacgaccgt
     3481 ccagcgatcc tcgcgcagca gggtctgata cgtgccgacg ttgaccatcg gctcgataca
     3541 gacgaccatg ccgacctgcc agcggacgcc ccgccccggc tcgccgtagt tcgggatctt
     3601 ggggtcctcg tgcgggcggc ggccgatccc gtggccggtg taatcgcgca cgacggcgaa
     3661 gccgttgcgc tcgacgtggg cctggacggc ggcggaaaga tcgcccatcc gcaggcccgg
     3721 gcggaggaat tcgatgccga tttccagcgc ctcgccggtg acctggatca accggttgga
     3781 gacctcatcg gcgcggccga cggggacggt gcgcgccatg tcggcatagt agccgtcgag
     3841 gatcaggccg aggtcgaggc tggcgatgtc gccctcgacc aggacgcgct tgcccgggat
     3901 gccgtgcacg atctcctcgt tgatcgagat gcagagcgag ccggggaaat cgtagaggcc
     3961 gaggaaggcg gggattgccc cgcggcgctc gatctcgtgt ctggccaccc ggtccagttc
     4021 accggtggtg acgccggggc ggacgtggcc gatcacctcc tccatgactt cgcgcaacag
     4081 ggcgccggcg cggcgcatcg tgttgagctc gtccttcgat ttgatcgtga tccggttggc
     4141 caaggctgct gctccggctc ggtccggcgt cgtggccggc tcaaagggtg ctcgggctgc
     4201 cgtgcatcag cggctcgagc acctggcgga tgtggtggaa cacggtttcc gggttgccct
     4261 cggccgggat ccggtagaaa tagtgctcct tctgcatctc cgccagaatc ggctcggttt
     4321 cgttgtggta gacgcgcatc cgttcgcgga tggcgtcgac ggcgtcatcg gggcggatga
     4381 tcagctcgcc gttgcacttg tcgcagcggt tggggaccgt cggcggcaga tcgagcagat
     4441 ggtagaccgc gccgcaggtc ccgcagatgc gtcgtccggt gagccgttcc accagactgt
     4501 cgtcgtcgat ctggaggtag agcatggcgt tcaggggaac ggcccaagcc tccagtttcc
     4561 cgatcagatg ccgcagttgg ggcaccgtgc ggggatagcc atcgagcagg acgccgtcgc
     4621 catggtcgat ggtggacttg aggcgccgaa ccatgacctc gttgatgacg gggtccggca
     4681 ccagattgcc gtggctcatc agctcaccgg cgagtcggcc caggggcgtc tgctggcgga
     4741 tttcatcgcg caggatgccg cccgaggaga ggtgcgcgaa gtggaacgtg cgctggatgc
     4801 gctcggactg ggtgcccttg ccacagccgg ggggccccag aatgccgatt accattcgct
     4861 ggggaaccgg gccaccttcg ggcattcagc cctcctcgtc atattgccgg actgttcgcc
     4921 gcgcggcgcg gccggggcga tcacgcaccc cgcatgcgga cgacgggccg ccgcgagcgg
     4981 ctgccgacgc gtccaccgct ccggccaaat ccttcgtaat ggcgcatcat caattgcgac
     5041 tcgatctgca tcatcgtatc gagcacgacg cccacgacga tgatcaggcc ggtgccgccc
     5101 gccatctgcg ccacgccgaa cgggatgttg aagctgacgt agagcatgtc ggggacgagc
     5161 gcgacgacga tcaggaaaaa cgccccgacg acggtgatgc gcaccagaat atgatcgata
     5221 tagtcggcgg tgtgcttgcc cggcttgatc cccgggatgt aggcgccgct cttcttcagg
     5281 ttgtcggcca ggtcgaccgg attgagcgtg atggcggtgt agaagaaaca gaagaaggcg
     5341 gtcagcgcga tataggccag cgtgtagatg ttgaccatct tgagcaggct gaagatgccg
     5401 cccgaggtca gattgaaggc attgtagggg ttgtggatcg agttcatctc gagccagctg
     5461 ctgatggtga agccgatcga gccgggggcg ccgccgccga acatcggcag aatcatcgcc
     5521 ggaaaggtca ggatcgccga ggagaagatc accgggatga cgccggcggt gttcagcttg
     5581 agcgggatga acgtgttgcc gccctggacc attttgcgcc cgaccatccg gcgggcgtgc
     5641 tggaccggga ccttgcggtt ggcctgctgg ccgaagacga tcagcgccgc caccagcacg
     5701 ccgagcgcga ggaccatcat cagcccgatg ggggtcatcg agttgttgcg gatcgacatc
     5761 agggccaggt tcaggtcgat cggatagcgg gcgatgatcc cgatcgtgat gatgatcgag
     5821 atgccgttgc cgatgccgtg ctggctgatc ttctcgccca gccacatgat gaagcaggtg
     5881 ccggtgcaca tcgtcatcgc ggtgacgaag aggaacaggg cggggtgatc ggggatcagc
     5941 gagagttgtt cgttgagcag atagatgccg atgccgaagc cctggaacag actcaggcca
     6001 acggtgccgt agcgggtcca ctgggtgatt ttcttgcggc cggcctcgcc ttccttgctg
     6061 atcttttcca gccggggcca gacggccgtg aggagctgga ggatgatgga ggccgagatg
     6121 tagggcatga tgcccagggc gaagacggtc atctggctga aggcgccgcc gctgaacatg
     6181 tcgacgacgt tgaacacgcc gccgctgccg aaggttccgc gaaagaactc gcggatcttg
     6241 accggatcga tgaacggcgt ggggacgtgg gcgccgatgc ggtagacgag aatcagcccg
     6301 agcgtgatga gcaccttgcg gcgcaggtcg gggacgcgga agatgttgat aagggaaccc
     6361 aggatcatgg tcagatctct tcgcagcggc cgccggcggc ctcaatggcc gcgcgcgcgg
     6421 cggaagagaa accgtgcgcc ctgacggtca gttttttctc cagcttgcca ttggcgagga
     6481 ccttgatgcg gcgggcctgg cgcgagacga tgccggcggc gagcatcgcc tcgtgatcga
     6541 cggtggcgcc gtcctcgaag ccgttcaggt cgcgcagatc gacttcgtcg cgccggaccc
     6601 ggaaccggtc gttgttgaag ccgaacttgg ggaggcggcg gatgaggcgc atctgcccac
     6661 cctcaaaggc ggggcccttg ccgctgccgg agcgggcctg ctggcccttg tggcccttgc
     6721 cggcagtctt gccctggccc gagccctcgc cgcggccgac gcgcttgggc tgctgctttc
     6781 ttcccggatc gccgggcagc tcgtgcatct tcaacatgat cagtcgacct cctcgacccg
     6841 aaccagatga ttgacggcga acaccatccc gcggacgggg tcggaggcgt ccttgacgac
     6901 gctctggttg agccgatgca atccgagcgc gcggatcgtg cgttgatgtt gcttcttggc
     6961 gccgatcgcg ctcttgaccc aggtgatttt cagtttgctc gccatcgctt cacattcctg
     7021 ccttaacggg atccagcggg tcggcctcac gatccgctgt cggggcggtc ttgttcctcg
     7081 gccggctgct cggcggggcg gccgggctcc gcgggcgggg tcgcctcggc gggcgagggg
     7141 gtttcggccg gcgccggggc ggccgccgcg gcggaaggcg gattcggcgc gccgctgtcg
     7201 ggcgccggag gctcggcggg ggcgggctgg ggggcggctt cggccggttg cggggcggcc
     7261 ggggccgggg ccggcgccgc ggcgcgcacg ccagcgggct gttccgcgga cgcggcgggt
     7321 ttctcctcgg ctttggagtc gccccgggca tcctcgccca ggacacgcgc cagcgtgtct
     7381 tcgtagatcg ccgcggcgcg cttgccgatc agttcgtcga tcttcttgcc gcgcaggcgg
     7441 gcgacatgct cgggccgctt gagcgaggcc agcccctgga gcgtcgcctt ggcgacgttc
     7501 atcacgttgt ccgagccgag gctcttggcc aggatgtcct ggacgccggc caattgaagc
     7561 accttgcgca cctgggcgcc ggcgatcagg ccggtacctt cggaagccgg cttgagcagg
     7621 acgcgggcgg tggagtagag gccatagact tcgtggggga cggtgcggcc gacgagcggg
     7681 acctggatca ggttcttctt gccattctcg accgccttgg cgatggcctc ggggacttcg
     7741 ttggccttgc cgaagccgag gccgacgtgg ccctcgccat cgccgacgac caccagcgcg
     7801 gagaaactga agcgccggcc gcccttgacc accttggcgc agcggttcag gacgatcaat
     7861 tcgtcgcgca ggttgagctt ggagggatcg atgcgggccg ccttgatgac gggcatcgcc
     7921 tcctcctgcg gttccttcgg ctcgcgattt ttctgctcgg tcgagttcat gctggcatcc
     7981 ttaaccttcg gttctctcaa aacttcaagc cactcttgcg cgccgcttcg gcgaacgcct
     8041 tgacgacgcc gtggtagcgg tagccgccgc ggtcgaagac gacctgctcg accccctggg
     8101 cgcgggcgcg ctcggcgatc agcgcgccga gcttctcggc ccaggcgcgg ttgctgaagt
     8161 tgcgcgcctc gctcttgttc gccttgttgt tggtggtcac gaagcagatc gtgcgcccgg
     8221 cgctgtcgtc gatcacctgg gcgtagatgt gcttcaggct cttgtggata cacagccggg
     8281 ggcgctcggg gctgccggcc accttgcggc ggacgcgcag gtggcgccga gtcagtgctt
     8341 cagtgcgtgt caaggccatc gtgcccaact cctcagtcgc tcaatcgatc cgcggtcgcg
     8401 cgggaccgcg ctgtcgccgt gccgcatcag acgccggcct tgcccacctt gcgccggatg
     8461 tgttcgccgc tgtagcggat gcccttgccc ttgtagggct ccggcgggcg gatgcgccgg
     8521 atattggccg ccacctggcc gacgagctgc ttgtcgatcc cgttgacggt gacgtgcgtc
     8581 gggtcgggga cggcgagggt gacgccgtcg atcggcccga aacgcacctc atggctgaaa
     8641 ccgaggttca tcacgagcgt cttgccgtcc atcgtggcgc ggtagccgac gccgaccagc
     8701 tcaagctgct tggcgaagcc ttcggcgacg ccggtcacca tgttgcggat caaccgctgg
     8761 tagaggccgt gataggcgcg gtcctgacgt tcgtcgctgt ggcgttcgac gagcagcgcg
     8821 ccgtcctcca ggcggacctt gacgtgattg tggaagtcca ggctcaggct gcccttgggg
     8881 cccttgacgg tcaggacgtt gccatcgatg cgggcctcgg cgccgcgggg caaggtgatg
     8941 ggaattttgc cgatacgaga cacggtaggt cactctcctg gatggcgtgg gcgccgcggc
     9001 gcttaccaga tgctgcagat gatttcgccg ccgacgttct ggcgccgggc ctcgcggccg
     9061 gtcatcacgc ccttgctggt gctgacgatc gtggtgccca tgccgccgag caccttgggg
     9121 atatcgtggc tgccggaata gcggcgcagg ccgggccggc tgacccgggt caggccgttg
     9181 atcaccttct cgccgcccgg gccgaacttc agatagacga tgagcaggtt gtgcgagagg
     9241 ggtttggcat cgcgcttgtc gcccgccttg ggattgcgct tgacgctgcc ggcgcggacg
     9301 accttgaagt atttgatgta gccttcctcg cgcaggatct tggcgatcgc gatcttcatc
     9361 ttgctggccg gcatgcgcac cgaatcctgc ttggcatgga tcgcgttgcg caggcgcgtc
     9421 agcatatcgg cgatggggtc ggtcacgact gacatgttcg tccggttcct cttcccttgg
     9481 cggttctcac caactcgact tggtcacgcc cgggatctgc cccaggctgg cgtacttgcg
     9541 gaagcacagc cggcacattt tgaattgccg catgaaggcg cgcgaacggc cgcacagcgg
     9601 gcagcgattg tgctggcgcg tcttgaactt gggctttctg agcgccttgg ctttcagaca
     9661 ggttctggcc acggaatcga tctccttcgt ccctcacgct aaaaatacgt cttctcgttc
     9721 ctggtccctc atccacgtga tcgagttgag cggttctgtc gtccctacgg gactctccgg
     9781 ttcttctttc ccgttttccc gccactgaag tggcgggctg aagtcttccg tccctggcgg
     9841 gactgggctt tgggcgaggc cggcgatgcc tcggctattc gatgcccgga tttatttttc
     9901 cttgtcctgg cttctcagtt cggcccccaa ggggcggctc aaatgccccc agcgggactg
     9961 ggctttgggc gaggccggcg atgcctcggc tattcgatgc ccggatnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnccca gcgggactgg gctttgggcg aggccggcga
    10141 tgcctcggct attcgatgcc ccgcgttctt tcgctccttg tcctcattct ccaatcggcc
    10201 ccgagggggc agctcaaatg cccctggcgg gactgggtca agcctcagtt ctcgcgcagc
    10261 ggcaggccga agcgccgcag cagcgtccgg cagtggtcgt cgcttttcgc attggtcacg
    10321 agcgtgatgt tcatgccgaa ggtctgctgc acctggcggt gatcgatctc ggtgaagatc
    10381 gtatgctcgc ggatgccgaa attgtagttg cccttgccgt cgaacgcccg cggggggagg
    10441 ccgcggaagt cgcggatgcg ggggatcgcg acgttgatca ggcgctcgag gaactcatac
    10501 atgtagggcc cgcgcagcgt gaccatgcag ccgacgggca tgccggcgcg cagtttgaac
    10561 gccgcgaccg agatcttggc ccgggtgcgc cggggcttct ggccgctgat cagggccagg
    10621 tcctcctcgg cggcctggag gatcttctca tcgcgcgagc cttcgccgac gcccatgttg
    10681 agcacgacct tgagcaggcg ggggacctcc atcacgttct tcagcttcag ctcgtccatc
    10741 aggctggggc ggatccgctc ctgataatcg gcgagcagcc ggggccggta gccgttctgt
    10801 gtcgctttgt cactcatcat ccacgctctt gggtgtcggt tggggggacg ggcagagtcg
    10861 cgccggtcag accgtcacct ccttggattc gccgcgcagc ttcaacacgc gctgcttgtt
    10921 gccgtgctcg tcggtcttga cgcggaagcg cgccgcgcgc ccgcgaccgc cggtctcgac
    10981 gagcatcagg ttgctcatgt ggatcggcgc ttccttctcg atgatgccgc cggcatcctg
    11041 ctggccgcgg gccttctggt gcttcttgac gcggttgacg ccctcgacga tcgcgcgctg
    11101 acgcgcggtg tcgacggcca gcacgcggcc gcgggcgccc ttgaaggcgc cggagatcac
    11161 cagaaccagg tcgtcgcgtt tgatgcgcag ggccatcgct ccactcccta gatgacttcg
    11221 ggcgccagcg aaacaatctt catgaagttt cgcttgcgca attcgcggcc gaccgcaccg
    11281 aagatacggg ttccaatcgg ctccatgttg gcgttgatca ggacggcggc gttgctgtcg
    11341 aagcggatgt aggagccatc ctcgcggccg acttccttga ccgtgcgcac gatgacggcc
    11401 ttgacgacct gacccttctt gatgttgctg tcgggggcgg cctcgcgcac cgaacagacg
    11461 atcacatcgc cgatgctggc gtagcgccgg cgcgacccgc cgagcacctt gaaacagcgc
    11521 agcttgcgcg cgccgctgtt gtcggccaca tccagtacgg tatattcctg aatcatcgtc
    11581 gtctcgcatt cccagcgttc tcggccgcgc cccggcggca cggcgcatcg ccagcgaagt
    11641 cggctacttc gcccgctcca gaatcgcgct gacgcgccag cgcttgtggc gcgacagcgg
    11701 ccgcgtttcg atcacgcgca cggtatcccc ttcgcggcag ttgttctcct cgtcgtgggc
    11761 atagagccgt ttggtctggc gcatcgtctt gccgtag
//
LOCUS       DAOPCO010000106        11780 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5368_length_11780_cov_4.831215, whole genome shotgun sequence.
ACCESSION   DAOPCO010000106 DAOPCO010000000
VERSION     DAOPCO010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_11780_cov_4.831215"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLS90_08945"
     CDS             complement(<1..346)
                     /locus_tag="PLS90_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02570.1"
                     /translation="MTSSEHNEPRNNLIALYGLISVVGLFLITFLLIYFFSFWRQTIV
                     SAIVEGAPTPELRDLRAREDTALNKYAYLDAATGTVAIPIERAIELEAAAPWRAAMDL
                     PTKVTPEATATPT"
     gene            complement(360..1574)
                     /locus_tag="PLS90_08950"
     CDS             complement(360..1574)
                     /locus_tag="PLS90_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HPK02571.1"
                     /translation="MMRPEAQVDYRLKGSHPQLVWRVLLALAIASLVLGLVFSFAGAD
                     DSEAWRQFKFSYVTSYACYLSLSLGALFFVLIQHSCNARWSVTVRRVSEAIMMNMGLM
                     LVLLVPILFWLPDLYHWADAEHVAHDALLQQKAPYLNIPFFLIRMGLYFVIWIGLSTY
                     LYNRSVRQDETGDYGLTQAMRRISPVGLILFSLSLTYFAFDLLMSLDAHWYSTIFGVY
                     YFSGAFMGALAFMILICIWVCSTGAVGRAINREHFHDLGKLMFGMVVFWAYIAFSQLL
                     LIWMGNIPEETVWYMHRYTGTWKGFSWFLLIGHFILPFLYLLPRSVKRNQALLGIGAL
                     WMLAMHYLDMYYLAMPSFTHENVPFNIVDPALFIGMGCLFAAAVVWRLTRRHVVPIRD
                     PYLAESLAFENF"
     gene            complement(1571..2188)
                     /locus_tag="PLS90_08955"
     CDS             complement(1571..2188)
                     /locus_tag="PLS90_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPK02572.1"
                     /translation="MSSPIRIMVLLLSAALLGGCRGRVTDEPPVHLNPNMDDQEKLDP
                     QEEFSFFPDGRASREPVAGTIARGDLQVDPVRYEGQTAAGNWIQHGPLPTTEELLRRG
                     QRQFNIYCAPCHDRVGTGRGMVTNYAGMTPPTNLQDARIREMPDGQIFHTITYGVRTM
                     PSYRHQVGVTDRWAIVAYLRALQRSQSTTLADVPSDMRSTLEVRP"
     gene            complement(2193..2768)
                     /locus_tag="PLS90_08960"
     CDS             complement(2193..2768)
                     /locus_tag="PLS90_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HPK02573.1"
                     /translation="MRLPHFLRLVTGQLPEDNPFGIVAEFDNPGALLHAAEAVRKAGY
                     RAFDVHSPFPIHGMDEAMGVRRTILPFLVLGGGLTGCVGALAMQIWMMAYDYPLVISG
                     KPLMSIPAYIPVTFELTILLSGFAAVGSMLFLNFLPMLYHPLFKHEAFRKATDNAFFL
                     SIEARDKQFDVEGTRSLLESIGGRNITLLEP"
     gene            complement(2775..4190)
                     /gene="nrfD"
                     /locus_tag="PLS90_08965"
     CDS             complement(2775..4190)
                     /gene="nrfD"
                     /locus_tag="PLS90_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020147620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPK02574.1"
                     /translation="MAVAHTNPPLQQAPPDEPLVAGGHDFASATETVSRVAESRTPKG
                     WYVAFAIAVTFLGVLAVVISYLLWEGVGVWGVNNPVGWGWAIVNFVWWIGIGHAGTLI
                     AAILFLFRQKWRTSINRFAEAMTIFAVACAGIFPGIHVGRVWVAYWLFPIPNQMGIWP
                     QFRSPLLWDVFAVSTYATISVLFWYVGMVPDLATLRDRAKTPLRRIAYGIFALGWRGS
                     HRHWHVYEVAYLVLAGVSTPLVLSVHSVVSFDFAVSVLPGWHTTIFPPYFVDGAIFSG
                     FAMVMTLMIPARQWFGLKDFVTLRHLENMNKILLATGMIVGYAYSTEFFIAWYSGNLY
                     ERFTFIHRALGPYAWAYWIMISCNVISPQLFWFRRLRRSIPVMFVVSIFVNIGMWFER
                     YVIVVTSLASDFLPSSWDYFSPTLIDVLTYLGSFGLFFTLFLLFLRFLPVLALAEIKA
                     IMPQAHAHHGHEPAHAGHATE"
     gene            complement(4194..7235)
                     /locus_tag="PLS90_08970"
     CDS             complement(4194..7235)
                     /locus_tag="PLS90_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HPK02575.1"
                     /translation="MAHAPDKAPETLGRGLNERLTDAHGRRYWRSLDELENTPQFQEF
                     LHREFPEGAAEMLSPFTRRAFLNLMGASIALAGGLTGCRRPEHKILPYAKAPENVLPG
                     IPKYYATTMERAGAAVGLIVECHDGRPTKIEGNPDHPGNLGAVDPITQAEILELYDPD
                     RSTGVLHENRPATREQFDAFARERFSELRRNGGAGLHILGELKSSPSFRAVRERFQAD
                     FPRSRWHVWEPVNEDNVAAGTALALGRAAHPAVDFENADVVLALGSDFLASGPTWMAD
                     ARRFARRRVPEGERPMNRLYAVEGAFTLTGAMADHRLRLAPSQIPLVALVLMNEISGG
                     ENAALAGPLLANVEGLDVAWLQAVARDVAAHPGRAAVLVGAEQPPAVHAAAAVLNGVI
                     GSECVAYRPYPNEDYGRAGRSIAELAAALEAGEVDTLVILGGNPVYDAPADLNFAERL
                     SRAAHVIHLSYHVNETSRHGAVQWHVPRAHALEAWGDTMAFDGTVALQQPMIAPIYDG
                     LSDAELMALLGGQPLTRGYDILRAFHRGDEAEGPAFEARWEQMLHDGLVPGTAGTIAA
                     LGAPAAGSASVGAELAEAASAAAPTGDAMELIFQADHHLFDGRYANNGWLQEAPDPVT
                     RISWDNAALISPATAEALRAPLGTYINIEVDGQSVKIPAWTLPGMADNVVVVRLGYGR
                     ADAGRVAEGVGFNVYPLRTLAGLHVRTGVKVTRAGGHFDLANTQDHWSIEGRPIYRQT
                     TGALYAEHPLIIKEMEAETPSHNGQTQPFREFEYDGEQWGMAIDLSTCIGCNACLVAC
                     QAENNIPIVGREQVNNGREMHWIRVDRYFSGQRLEDVEMVFQPMPCQHCENAPCEQVC
                     PVAATTHSPDGLNEMTYNRCVGTRYCSNNCPYKVRRFNFFNYHEDYPDLLKMASNPNV
                     TVRMRGVMEKCTYCVQRIRRDQLRRRGTDEPYREGSVRTACQTACPTEAIVFGNINDE
                     TSLVARAKRLNRNYEVMGNLNFRPRTSYLARLRNPNPELEA"
     gene            complement(7341..7982)
                     /locus_tag="PLS90_08975"
     CDS             complement(7341..7982)
                     /locus_tag="PLS90_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK02576.1"
                     /translation="MSTVFPRWVNRLPFLMIFIGGGGLFGVVGFVWYYFSPWYTDVGY
                     APRQPVPYSHKLHAGTLGIDCRYCHTGVEVGQHATIPPTQTCMNCHTMIRPESPRLLP
                     VRESFATGEPVEWLRVHKLPDYVRFSHQAHVQAGVGCVECHGRVDQMEVVRQTKPLSM
                     GWCLDCHRAPEAALRPPEMVTVMDYVATAPDEGARLRAAGQIEPPTHCSACHY"
     gene            8578..9582
                     /gene="sppA"
                     /locus_tag="PLS90_08980"
     CDS             8578..9582
                     /gene="sppA"
                     /locus_tag="PLS90_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPK02577.1"
                     /translation="MRRLNPLLLVPLLFALPACITLPPLGIGNDRGRLSMEIVRPAEH
                     WMTHDQILMIPLDGLVREGDTSTYFGEVGMLVELKDRLLAAERNRRIKAIVLRIDSPG
                     GTVTAADLIHQELERFKERTGLPVIAHLNATAASGGLYIAMAADEIYALPTTLTGSIG
                     VIVTLPKVHKLADKIGFEMAVVKSGENKDLGSPWQPMSPAERAIFQNIIDNYYARFLD
                     VILASRAPRGMTEEGLRALADGRVFGPETALEAKLIDGILYPDAVFDRAEELAGIADA
                     EIITYEYPYTWRGNIYARSDSPNARPPAAGGDLNLIKLELGQIESLLGASRFHYLWLP
                     "
     gene            9616..10863
                     /gene="argJ"
                     /locus_tag="PLS90_08985"
     CDS             9616..10863
                     /gene="argJ"
                     /locus_tag="PLS90_08985"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPK02578.1"
                     /translation="MSKRDGQTASTADEIPPLRGPKGFRAGGIAIGLKASGRLDLAAI
                     VSDRPATAAGVMTQNLFCGAPVTVARQRLAASPRLRGLIVNSGIANACTGEQGVLDAV
                     AMAGALEGAIGAAPGECLAASTGVIGHRLPIDRIAAETPRLVDSLADDGFGVFAHAIM
                     TTDLQPKWARRTVRMGAFPRSGPSVTILGVAKGSGMIEPNMATMLAFITTDYPLGPGQ
                     ARQIVRRVADATFNCVTVDGDTSTSDMLLLLANGAAMERRTTDAARDERFEAALREVC
                     ESLARQIARDGEGANHLVTIEVNGAPDDDGARRIAKAIANSKLVKTALFGHDPNWGRI
                     CCAAGYAGVPFKPEEFDLKLQGRAVMRAGLPARFDRAALAHALRKEHILIQATVGPGR
                     GRARVWTCDLSYDYVRINAEYTT"
     gene            11098..>11780
                     /locus_tag="PLS90_08990"
     CDS             11098..>11780
                     /locus_tag="PLS90_08990"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02579.1"
                     /translation="MTPRPDTPDTPDTSSTPSTPTAPTPSPPSPHADLPPDALASPLA
                     RAGLEVARRLQAAGHQACFVGGCVRNALLGLPVKDIDLATSARPDQVAALFERCESVG
                     AHFGVQIVVLEDFQFEVATFRAEGAYVDHRRPTQVRYGSFDDDWRRRDFTVNAIYYDP
                     LARRLLDPAGGRDDLAQRRLRAVGDPDLRFAEDALRLLRAVRFAGRYDLTLEPATAAA
                     IRRHGATLRA"
BASE COUNT         2017 a         4619 c         3265 g         1879 t
ORIGIN      
        1 gcgtcggcgt cgccgtcgcc tcgggcgtca ccttcgtcgg cagatccatg gccgcccgcc
       61 acggcgcggc cgcttccagt tcaatcgccc gctcgatggg gatcgccacc gtccccgtgg
      121 cggcatccag atacgcatac ttgttcaggg ccgtgtcttc cctcgcgcgc agatcgcgca
      181 gttccggcgt gggcgctccc tccacgatag cactgacgat cgtctgccgc caaaacgaaa
      241 agaagtagat cagcaggaac gtgatcagga acagccccac cacggagatc aggccataca
      301 gcgcgatcag attgtttctc ggttcattat gctcggaact tgtcatgcgg gccgtcgcct
      361 tagaaattct cgaaagctaa cgattcggcc agatacggat cgcgaatcgg cacgacatgg
      421 cgccgcgtca gccgccacac caccgccgcc gcgaacaggc accccatccc gatgaacagc
      481 gccggatcga cgatgttgaa cggcacgttc tcatgcgtga acgacggcat ggccaggtaa
      541 tacatgtcca ggtagtgcat cgccagcatc cacagcgccc cgatccccag cagcgcctgg
      601 ttgcgcttga ccgaccgcgg caacagatac aggaacggca ggatgaagtg gccgatcagc
      661 aggaaccagc tgaacccctt ccacgtcccc gtgtagcggt gcatgtacca caccgtctcc
      721 tccgggatgt tccccatcca gatcagcagg agctgcgaaa acgcgatgta ggcccagaac
      781 acgaccatgc cgaacatgag cttgcccagg tcgtggaaat gctcgcggtt gatcgcccgg
      841 cccaccgccc ccgtcgagca cacccagatg cagatcagga tcatgaacgc cagcgcgccc
      901 atgaacgccc cggaaaaata atacacgccg aaaatcgtcg aataccagtg cgcgtcgagc
      961 gacatcagca ggtcgaacgc gaaatacgtc aggctcagcg aaaacagaat cagccccacc
     1021 gggctgatcc gccgcatcgc ctgcgtcagc ccgtaatccc ctgtttcgtc ctgacgcacc
     1081 gaccggttgt aaagatatgt gctcagcccg atccagatca cgaaatacag ccccatgcgg
     1141 atcaggaaga acggaatatt cagatacggc gccttctgct gcaacagggc gtcgtgcgcc
     1201 acgtgctccg cgtcggccca gtgatacagg tcgggcagcc agaacaggat cggcacgagc
     1261 agcaccagca tcagccccat gttcatcatg atcgcttcgc tcacccgccg caccgtcacg
     1321 ctccagcgcg cgttgcaact gtgctggatc agcacgaaga acagcgcgcc gagcgacagc
     1381 gacaggtaac acgcatagct cgtcacgtac gaaaacttga actgccgcca ggcctcgctg
     1441 tcgtccgcac cggcgaagct gaagaccagc ccgagcacca gcgacgcgat cgccagcgcc
     1501 agcagcaccc gccacaccag ttgcggatga ctccccttca ggcggtaatc gacttgggct
     1561 tcgggacgca tcatggcctc acttcaagtg tcgagcgcat gtcggacggc acgtccgcca
     1621 gcgtcgtgga ctggctgcgc tgcaacgcgc gcaggtaggc cacgatcgcc cagcgatccg
     1681 tcaccccgac ctgatgccgg tacgacggca tcgtccgcac cccatacgtg atcgtgtgga
     1741 agatctgccc gtcgggcatc tcgcggatgc gcgcgtcctg caggttcgtc ggcggcgtca
     1801 tcccggcgta gttggtcacc atcccgcgac ccgtgccgac ccggtcgtgg cagggcgcgc
     1861 agtagatgtt gaactgccgc tggccccgcc gcagcagttc ctccgtcgtc ggcagcggcc
     1921 cgtgctggat ccagttgccc gccgccgtct gaccctcgta gcgcaccggg tccacctgca
     1981 gatcgccccg cgcgattgtc cccgccaccg gctcgcgcga cgcccgtccg tccggaaaaa
     2041 acgaaaactc ctcctgcggg tcgagcttct cctggtcgtc catgttcgga ttcagatgca
     2101 ccggcggctc gtcggtgacc cggccgcggc atccccccag cagcgcggcg ctcagcagca
     2161 ggaccatgat gcggattggc gaactcatcg gctcatggct ccagcagcgt gatgttgcga
     2221 ccgccgatcg actcgagcag cgaccgtgtt ccctcgacgt cgaactgctt gtcgcgcgcc
     2281 tcgatcgaca ggaagaacgc gttgtccgtc gccttgcgga acgcctcgtg cttgaacagc
     2341 gggtgataca gcatcggcag aaaattgagg aacagcatgc tccccaccgc cgcgaatccc
     2401 gacagcagaa tcgtcagctc gaacgtcacc ggaatgtagg ccgggatgct catcagcggc
     2461 ttgccgctga tcaccagcgg atagtcgtag gccatcatcc agatctgcat cgcgagcgcg
     2521 ccgacgcagc ccgtcagccc gccccccagc accaggaacg gcaggatcgt ccgccgcacc
     2581 cccatcgcct catccatccc gtggatcgga aacggcgaat gcacgtcgaa cgcgcgatag
     2641 cccgccttgc gcaccgcctc ggccgcatgc agcagcgcgc ccggattgtc gaactccgcc
     2701 acgatcccga acggattgtc ctcgggcagt tggccggtca ccaggcggag gaaatgcggc
     2761 agtctcatcc tcggctactc cgtcgcgtgg cccgcatggg ccggttcgtg gccgtggtgc
     2821 gcgtgggcct gcggcatgat cgccttgatc tccgccagcg ccagcaccgg caggaagcgc
     2881 aggaacagca ggaacagcgt gaagaacagc ccgaagctcc ccaggtaggt cagcacgtcg
     2941 atcagcgtcg ggctgaaata gtcccagctc gacggcagaa agtcgctcgc cagcgacgtc
     3001 accacgatca cgtagcgctc gaaccacatc ccgatgttca cgaagatcga gacgacgaac
     3061 atcaccggga tcgagcggcg cagccggcgg aaccagaaca gctgcggcga aatcacattg
     3121 cacgaaatca tgatccagta ggcccatgca tacggcccca gcgcccggtg gatgaacgtg
     3181 aaccgctcat acaggttgcc gctgtaccac gcgatgaaga actccgtgct gtaggcgtag
     3241 cccacgatca tccccgtcgc cagcaggatc ttgttcatgt tctccagatg ccgcagcgtc
     3301 acgaaatcct tcagcccgaa ccactggcgc gccgggatca tcagcgtcat caccatcgcg
     3361 aacccggaaa aaatcgcccc gtcgacgaaa tacggcggga agatcgtcgt gtgccatccc
     3421 ggcagcaccg acaccgcgaa gtcgaacgac acgaccgagt gcaccgacag caccagcggc
     3481 gtcgaaaccc ccgccaggac cagatacgcc acctcgtaca cgtgccagtg ccggtgcgac
     3541 ccgcgccagc ccagcgcgaa aatcccatac gcgatccgcc gcagcggcgt cttcgcccgg
     3601 tcgcgcagcg tcgccaggtc cggcaccatc cccacatacc agaacagcac cgaaatcgtc
     3661 gcatacgtgc tcaccgcgaa cacgtcccac aacagcggac tgcggaactg cggccagatc
     3721 cccatctggt tcggaatcgg gaacagccag taggcgaccc acacgcggcc gacgtgaatc
     3781 cccgggaata tcccggcgca ggccaccgcg aaaatcgtca tcgcctcggc gaaccggttg
     3841 atcgacgtcc gccacttctg ccggaacaga aacagaatcg cagcaatcag cgtccccgca
     3901 tggccgatgc cgatccacca cacgaagttg acgatcgccc agccccagcc gaccgggttg
     3961 ttcacccccc agacgccgac gccctcccac agcaggtagc tgatcaccac cgccagaacg
     4021 ccgaggaacg tcaccgcgat cgcgaacgcc acataccacc ccttcggcgt ccggctctcc
     4081 gccacccggc tcaccgtctc ggtcgccgag gcgaaatcgt gccccccggc caccagcggt
     4141 tcgtccggcg gcgcctgctg cagcggtgga ttggtgtgcg cgacggccat cgatcaggct
     4201 tccagctcgg gattcgggtt ccgcagacgc gccagatacg aggtccgcgg tcggaaattc
     4261 aaattcccca tcacctcata gttgcggttg agccgcttcg cccgcgccac caggctcgtc
     4321 tcgtcgttga tgttgccgaa cacgatcgcc tcggtcgggc acgccgtctg gcacgccgtc
     4381 cgcacgctcc cctcgcgata cggctcgtcc gttccccgcc gccggagctg gtcgcgacgg
     4441 atccgctgca cgcaatacgt gcatttctcc atcacgccgc gcatccgcac cgtcacgttc
     4501 gggttcgacg ccatcttcaa cagatccggg tagtcctcgt ggtaattgaa aaagttgaac
     4561 cgccgcacct tgtacgggca gttgttcgag cagtagcgcg tcccgacgca ccggttgtac
     4621 gtcatctcgt tcagcccgtc ggggctgtgc gtcgtcgccg ccaccgggca cacctgctcg
     4681 cacggcgcgt tctcgcagtg ctggcacggc atcggctgga acaccatctc cacatcctcc
     4741 agcctctggc cgctgaagta gcggtccacc cggatccagt gcatctcccg cccgttgttg
     4801 acctgttccc ggccgacgat cgggatgttg ttctcggcct gacacgccac caggcaggca
     4861 ttgcatccga tgcacgtgct caggtcgatc gccatccccc actgctcgcc gtcatactca
     4921 aattcgcgaa agggctgcgt ctgcccgttg tgcgacggcg tctccgcctc catctccttg
     4981 atgatcagcg ggtgctcggc atacagcgcg ccggtcgtct gccggtaaat cggccggccc
     5041 tcgatcgacc agtgatcctg cgtgttggcc aggtcgaagt gcccccccgc ccgcgtgacc
     5101 ttgacccccg tccggacgtg cagccccgcc agcgtccgca gcggatagac gttgaagccc
     5161 accccctcgg ccacgcggcc ggcgtcggcc cggccgtagc ccagccgcac caccacgacg
     5221 ttgtcggcca tccccggcag cgtccacgcc ggaatcttca cgctctggcc gtccacttcg
     5281 atgttgatgt aggtgcccag cggcgcgcgc agcgcctcgg ccgtcgccgg actgatcagc
     5341 gccgcgttgt cccacgaaat ccgcgtcacc ggatcgggcg cttcctgcaa ccagccgttg
     5401 ttggcgtagc gtccgtcgaa cagatgatgg tcggcctgga agatcagctc catcgcgtcg
     5461 cccgtcggcg cggcggccga cgccgcctcc gccagctccg ccccgaccga cgccgaaccc
     5521 gccgcgggtg ctcccagcgc cgcgatcgtc cccgccgtcc ccggcacgag cccgtcgtgc
     5581 agcatctgct cccagcgcgc ctcgaacgcc ggaccctccg cctcgtctcc ccggtggaac
     5641 gcccgcagga tgtcgtaccc gcgcgtcagc ggctggccac ccagcagcgc catcagttcc
     5701 gcatcggaca gcccgtcgta gatcggcgcg atcatcggct gctgcagcgc gaccgtcccg
     5761 tcgaacgcca tcgtgtcgcc ccacgcttcc agcgcgtgcg cccgcggcac gtgccactgc
     5821 accgccccat gccggctcgt ctcattcacg tgatagctca ggtgaatcac atgcgccgcg
     5881 cggctcaacc gctcggcaaa attcagatcg gccggcgcgt catacaccgg gttccccccc
     5941 aggatcacca gcgtgtcgac ctcgcccgcc tccagcgccg ccgccagctc cgcgatcgac
     6001 cggcccgccc gcccgtagtc ctcgttcgga tacggccggt acgccacgca ttcgctcccg
     6061 atcacgccat tgagcaccgc cgcggccgcg tgcaccgccg gcggctgctc cgcaccgacc
     6121 agcaccgccg cgcgtccggg gtgcgccgcc acgtcgcgcg ccaccgcctg cagccatgcc
     6181 acgtccagcc cttccacgtt cgccagcagc ggcccggcca gcgccgcgtt ctccccgccg
     6241 ctgatttcgt tcatcagcac cagcgccacc agcgggatct ggctcggcgc cagccgcagc
     6301 cggtggtcgg ccatcgcgcc ggtcagcgta aacgcccctt ccaccgcata cagccggttc
     6361 atcggtcgct cgccctcggg aacgcgccgc cgcgcaaacc gccgcgcgtc ggccatccac
     6421 gtcggcccgc tcgccagaaa atccgacccc agcgcgagga cgacgtccgc gttctcgaaa
     6481 tccaccgccg ggtgcgccgc ccgacccagc gccagcgccg tccccgccgc cacgttgtcc
     6541 tcgttcaccg gctcccacac atgccagcgc gaccgcggga aatcggcctg gaaccgctcg
     6601 cgcacggccc ggaacgacgg cgaggatttc agttcgccca gaatgtgcag tcccgccccg
     6661 ccgttgcgcc gcagctcact gaaccgctcg cgcgcgaacg catcgaactg ctcgcgcgtc
     6721 gccggccggt tttcatgcag cacccccgtc gaacggtccg ggtcatacaa ctcaagaatc
     6781 tcggcctgcg tgatcggatc gaccgccccc agattgcccg ggtgatccgg gttcccctcg
     6841 atcttcgtcg ggcggccgtc atggcactcg acgatcagcc ccaccgccgc gccggcccgc
     6901 tccatcgtcg tcgcataata cttcggaatc cccggcagga cgttctccgg cgccttcgca
     6961 tacggcagga tcttgtgctc gggacgccgg cagcccgtca acccgcccgc cagcgcgatc
     7021 gacgccccca tcagattcag aaacgcccgc cgcgtgaacg ggctcagcat ctcggccgcc
     7081 ccttcgggaa actcccgatg cagaaactcc tggaactgcg gcgtgttttc cagctcgtcc
     7141 aggctgcgcc agtagcgccg gccatgggcg tcggtcaacc gctcgttcag gccgcggccc
     7201 agcgtctcgg gggctttgtc cggtgcgtgt gccatgcttg cagcgcgtgc tctttcaaat
     7261 tgcgggtcgt tccacccgac ggtttcatcc tcgtccatcc gcgcgcgggc cgccgcccgg
     7321 ccgcgccgcg ccccgcccgc ctagtaatgg caggccgagc aatgcgtcgg cggctcgatc
     7381 tgccccgccg cccgcagccg cgccccctca tccggggccg tcgccacgta atccatcacc
     7441 gtcaccatct ccggcggccg cagcgccgcc tccggcgccc ggtggcaatc caggcaccag
     7501 cccatgctca gcggcttggt ctggcgcacg acctccatct gatccacccg gccgtggcac
     7561 tccacgcagc ccacgcccgc ctgcacatgc gcctggtgcg aaaagcgaac ataatcgggc
     7621 agcttgtgca cccgcagcca ttccaccggc tccccggtgg cgaaactctc ccgcaccggc
     7681 agcagccgcg ggctctccgg ccggatcatc gtatggcagt tcatgcaggt ctgcgtcggc
     7741 gggatcgttg cgtgctggcc cacttcaacc cccgtgtggc agtaacggca gtcgatgccg
     7801 agcgtcccgg cgtgcagctt gtgactgtag ggcaccggct gccgcggggc atagccgaca
     7861 tcggtgtacc agggcgagaa gtaataccag acgaagccga ccacgccgaa caacccaccc
     7921 ccgccgatga agatcatcag aaagggaagc cggttgaccc acctgggaaa gaccgtggac
     7981 aagatggaag tccctgccct ctaacggagt tcaaatgaat gcgaacggtt tggcgccgcg
     8041 atgaccgatc cggttgctgg gaattgatcg cccggaagct tgcccgagaa tcgcgccata
     8101 tcgtaatgag gccatttcgg aaaatccacc acaaaatgct gtcctgcagg tcatcaatat
     8161 cggaccccaa ggcccgagtt ccctccccgt tttcccccaa aaaaccagca aatccgcccc
     8221 ctcagcccct tccctcccgc ttcccatttc ccctcttcca cccccctgtc tgtgaaaaaa
     8281 gtctctgatc tcccccgccc tcttgtgatt cttttcactg actctccccc gcaagcatcg
     8341 ctcctcactc caattgcggc tctcggcctc gtcctgtcct cgtgatcgtc cccgaagtcc
     8401 tgtcccaaga ctcggctcct gtccctcgtc ctcggctttc gaaatccttg ttcttcttcc
     8461 ccacccccgg gcctccttct ggcatcttcc cgcccggatg tgttagtttg ctgtggcgat
     8521 tgcccggacc gttcgcatcc accccgcatc ggcccgatcc cccaaggttc tcctcgcatg
     8581 cgccgcctca atccgttgct cctcgtgccg ctgctgtttg ccctgccggc ctgcatcacc
     8641 ctcccgccgc tcggcatcgg caacgaccgc ggacgccttt ccatggaaat cgtccgcccc
     8701 gccgagcatt ggatgaccca cgaccagatc ctgatgatcc cgctcgacgg cctcgtgcgc
     8761 gagggcgaca cctcgaccta tttcggcgaa gtcggcatgc tcgtcgaact gaaggatcgc
     8821 ctcctcgccg ccgagcgcaa ccgccgcatc aaggcgatcg tcctccgcat cgattccccc
     8881 ggcggcaccg tcaccgccgc cgacctgatt catcaggaac tcgaacgatt caaggagcgc
     8941 accggcctgc ccgtcatcgc ccacctcaac gccaccgccg cttccggcgg actctacatc
     9001 gccatggcgg ccgacgagat ctacgccctt cccaccaccc tgaccggcag catcggcgtc
     9061 atcgtcaccc tgcccaaggt ccacaagctc gccgacaaga tcggcttcga aatggccgtc
     9121 gtcaaatcgg gcgagaacaa ggacctcggt tccccctggc agccgatgag ccccgccgag
     9181 cgcgccatct tccagaacat catcgataac tactacgcgc gcttcctcga cgtcatcctc
     9241 gccagccgcg cgcccagggg catgaccgag gagggcttgc gcgccctcgc cgacggccgc
     9301 gtcttcggcc ccgagaccgc cctcgaggcc aagctcatcg acggcattct ctaccccgac
     9361 gccgtcttcg accgcgcaga ggagctggcc ggcatcgccg acgccgaaat catcacctac
     9421 gaatacccct acacctggcg cggcaacatc tacgcgcgca gcgacagccc caatgcccgc
     9481 ccccccgccg ccggcggcga cctcaacctg atcaagctcg agctgggcca gatcgagtcg
     9541 ctgctgggcg cctcgcgctt ccattacctg tggctcccct gaacccggcg catcctctcc
     9601 gcaaaggcgc acagcatgag caagcgtgac ggacaaaccg cctccaccgc cgacgagatc
     9661 ccgcccctgc gcggtccgaa ggggttccgc gccggcggca tcgccattgg cctcaaggcc
     9721 tcgggccgcc tcgatctggc ggcgatcgtc agcgaccgcc ccgccaccgc cgccggcgtc
     9781 atgacgcaga acctcttctg cggcgccccg gtcactgtcg cgcgccagcg cctcgccgcc
     9841 tccccccgcc tgcgcggcct catcgtcaac tccggcatcg ccaacgcctg caccggcgag
     9901 cagggcgtcc tcgacgccgt cgccatggcc ggcgccctcg agggcgccat cggcgccgcc
     9961 ccgggcgaat gcctcgccgc ctccaccggc gtcatcggcc atcgcctccc catcgacaga
    10021 atcgccgccg aaaccccgcg cctcgtcgat tccctcgccg acgacggctt cggggtcttc
    10081 gcccacgcga tcatgaccac cgacctccag cccaagtggg cgcgccgcac cgtccgcatg
    10141 ggcgccttcc cccgttccgg accgtccgtc accatcctcg gcgtcgccaa gggctccggc
    10201 atgatcgaac cgaacatggc gaccatgctc gcctttatca ccaccgatta cccgctcgga
    10261 cccggtcagg cgcgccagat cgtccgccgc gtcgccgacg ccaccttcaa ctgcgtcacc
    10321 gtcgacggcg acacctccac cagcgacatg ctcctgctgc tcgccaacgg cgccgccatg
    10381 gaacgccgga cgaccgacgc cgcgcgcgac gaacgcttcg aagccgccct ccgcgaagtc
    10441 tgcgaatcgc tcgcccgtca gatcgcccgc gacggcgagg gggccaatca tctggtcacc
    10501 atcgaggtca acggcgcccc cgacgacgac ggcgcccggc gcatcgccaa ggctatcgcc
    10561 aacagcaaac tggtcaaaac cgccctcttc ggccacgacc ccaactgggg ccgcatctgc
    10621 tgcgccgccg gctacgccgg cgtccccttc aaacccgagg agttcgatct gaagctccag
    10681 gggcgcgcgg tcatgcgcgc cggcctcccc gcgcgcttcg accgcgccgc cctcgcccac
    10741 gcgctgcgca aggaacacat cctgatccag gccaccgtcg gccccggccg cggccgcgcc
    10801 cgcgtctgga cctgcgacct gagctacgac tacgtccgca tcaacgccga gtacaccacc
    10861 tgactctgga tccgtccctc gcccctcgtc ctcgtgttcg cccctcgcag cgccactctc
    10921 tgagtggcgc tgcccccaac ggcctccctc agaattcggc tctccgctct cgtccctcgt
    10981 cctcggctct cgaattccct ggccttcctg ttccctctca ctcttcttcc ctctcactcc
    11041 ctctccctct ccttcctcca cttccccacc gcctcccgaa tcgcttacaa tcatccaatg
    11101 accccccgtc ccgacacccc cgacactccg gacacttcct ccaccccgtc aacccccact
    11161 gcgccaaccc cctccccgcc ctcccctcac gcggatctcc cccccgacgc cctcgcctcg
    11221 ccgctggcgc gcgcgggact cgaggtcgcc cgccgtctcc aggccgccgg ccaccaagcc
    11281 tgtttcgtcg gcggctgtgt ccgcaatgcc ctgctcggcc tgccggtcaa ggatatcgat
    11341 ctcgccacct ccgcccgccc cgatcaggtc gcggcgctct tcgagcgctg cgagtcggtc
    11401 ggcgcccact tcggcgtcca gatcgtcgtc ctggaggact tccagttcga ggtcgccacg
    11461 ttccgcgccg agggcgccta cgtcgatcac cgccgcccca cgcaggtccg ctacggctcg
    11521 ttcgacgacg actggcgccg gcgcgacttt accgtcaacg ccatctacta cgaccccctc
    11581 gcccgccgcc tgctcgaccc cgccggcggc cgcgacgacc tcgcccagcg tcgcctgcgc
    11641 gccgtcggtg accccgacct gcgcttcgcc gaggacgccc tgcgcctcct ccgcgccgtc
    11701 cgcttcgccg gccgctatga cctgaccctc gaacccgcta ccgccgccgc catccggcgt
    11761 cacggcgcca cgctccgcgc
//
LOCUS       DAOPCO010000107        11772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5373_length_11772_cov_6.094137, whole genome shotgun sequence.
ACCESSION   DAOPCO010000107 DAOPCO010000000
VERSION     DAOPCO010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5373_length_11772_cov_6.094137"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLS90_08995"
     CDS             complement(<1..180)
                     /locus_tag="PLS90_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014769520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HPK02580.1"
                     /translation="MILQDDRRPTMSPLGADITAEQAQAVLEAARKKAEQLKLAMNIA
                     VVDAGANLKAFLRMDG"
     gene            630..941
                     /locus_tag="PLS90_09000"
     CDS             630..941
                     /locus_tag="PLS90_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02581.1"
                     /translation="MANAVQQDMNDVRSDLRQLREDLDSIKADLARLAHTAVDSGKDK
                     AHRARERAGEKLEDAYERVRHESERAFDCVQHSVEERPITSLLTAFGVGMLLGKIISE
                     K"
     gene            945..1229
                     /locus_tag="PLS90_09005"
     CDS             945..1229
                     /locus_tag="PLS90_09005"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPK02582.1"
                     /translation="MTELVELFLSAIDVIKAELRKSREGVYELAVIAGLVVCAFFLLV
                     VAFGLIVAGTFIGLVNSNVPVPLAALCTSVLCILIAWSLIWFGRERARRR"
     gene            1196..1450
                     /locus_tag="PLS90_09010"
     CDS             1196..1450
                     /locus_tag="PLS90_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02583.1"
                     /translation="MVWSGTSKTALGARRAEIPPENEAEAARMRLRAAAGGVPVETCV
                     RRRPWISLALALGAGVACGVSPALRRTAVTTAIALLRRCG"
     gene            1782..3833
                     /locus_tag="PLS90_09015"
     CDS             1782..3833
                     /locus_tag="PLS90_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK02584.1"
                     /translation="MPSQDPHTRRRSSTGAAPLRLLFVLAAGLLLGGLGGYLIGLRKT
                     PAPARHAAAQPPPPSAPRTLYTCGMHPEVISEEPGLCPKCEMKLTPMDPDRARLILEA
                     RGEGSATPAPPAGERNVLYWRSPMDPAFIRNEPGADAMGMDLVPVYEDEVAGGPSIRI
                     DPVTEQNMGLRYDVVRVGPLRKTFRTVGTVQYDEQSLGTVTTKVEGWVEKVHVQETGT
                     QVHAGDPLFDLYSPQLFSAQEEYLTALRDVERSSGTAADLARTRLRSAEERLRLFDIR
                     DEQIDQLRTTGEVSRTMTIHADLTGIVIERKVTEGDMLTPGMPAYRIADISTVWVIGR
                     IYESDLPFVRLGQEALMRLDYLPGRTWRGRVTYIYPYLPEGTREVPVRMEFHNPGYEL
                     KPGMYATIHLETTLAAEAVLAPAMAVIDTGERQIAFVAREPGKFEPRRVQTGARNDRD
                     EIQVLAGLAPGERVVVSGQFLLDSESRLREATLKMLQPGLVQTSEVLDHGAEPAARPG
                     AMAFERGTDAQPDAVDPVRYVCPMPSHAGILYDQPGDCPLCAMKLVPVRESDRPPAWV
                     AAHWTCPMPEHAHIEAEGPGNCPECGMTLIPVIPSAEAAKKSESVEATAGESAGIALY
                     VCPMESHMHIVSDQPGRCPECNMVLVPTSEVPFGKESELIWRALHGAGDEPTTSSAGA
                     R"
     gene            3833..4171
                     /locus_tag="PLS90_09020"
     CDS             3833..4171
                     /locus_tag="PLS90_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02585.1"
                     /translation="MNRVIARPIARIPAMFDSWSVFQPVVEDPFEQEALVECGGRKRA
                     ALGDAALDRSASAHVVGSKRCRRGALQRSAATLHKGCLDLAAQVKHWNSTRVSILAIR
                     IVNGHDSPDY"
     gene            4152..7601
                     /locus_tag="PLS90_09025"
     CDS             4152..7601
                     /locus_tag="PLS90_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK02586.1"
                     /translation="MIRRIIEFSLANKFIILLMMLAIVAGGIHAAKNIRLDAIPDLSD
                     VQVIILTEYAGQAPRIVEDQVTYPITTKMLAVPYAKVVRGYSFYNYSFVYVIFEDGTD
                     MYWARSRVLEYLSQLQSQLPDGVSPQLGPDATGVGWAFQYTLTSDARDLAQLRSLQDW
                     FLKYELTSIDGVAEVASLGGFERQYQVTVEPEKLRAYDLSITAVRDAIRKSNNEVGGR
                     TIEMAETDYMLRGMGYLGTLSPEEQRAAEARGIAIDLARMDKTIDELEAVALGVRADG
                     TPILLRDVGRVAAGPEMRRGVLEWNGKGEAVGGIVVVRYGADTLATIARVRERLAEIR
                     RSLPEDVRIHVGYDRTALIERAIATLRHTLIEESVIVSIIIILFLLHFRSALVPILTL
                     PLAVLISFVIMHYQGLGANIMSLGGIAIAIGAMVDASIIVVENVHKHYETKREDQPHW
                     ELVREACVEVGPTLFFALLVITVSFMPIFTLEAQEGRMFRPLAFTKTYAMAASAFLAV
                     TVSPVLAGFFIRRDVLPPRWSRRRRRLALLLTPPLVFLLLWLGTRRAGGGASGSALGL
                     SGWTWVALLVALATFLALLPQTMHPEERNPISRGLVRAYQPFLALLIRSRAVCWAIVV
                     AALLSALTIFFPYLHLGREFMPPLYEGDLLYMPTTLPGISATKARELLQQTDKIIAAF
                     PEVESVYGKIGRAETATDPAPMMMIETTIRLKDEAQWPAVEIRDDAGRVVDRRPRTSE
                     ELTDALNREIKFPGLTNAWTMPIKTRIDMLSTGIKTPVGIKIGGSDLATLERIGEQVE
                     AVVRDVPGTLSVYAERVMGDRYVNFIPDRQALQRYGLTVADVQEVFITAIGGMAIANT
                     VEGLERYSINLRYPRELRDNIDELKRVVVPTPQGFHIPLGDLGVFQIETGPAGIKSEQ
                     SRPNAWVYVDLDDIDVGTYVEHARAAVDAAIADGRIVLPAGYSIHWSGQYEYMVRARA
                     RLRAVVPLTLLIILVLIYLSTGSAFQTFVVMACLPFSLLGAFWMLYWLDYNLSVAVWV
                     GIIALAGLDAETSQVMLLYLDLAWDKLKRSGARLDWAAARAAVLEGAATRLRPKAMTV
                     GSTLFGLLPIMWASGAGADTMKRIAVPMIGGMITSFILEMAVIPVVYYLWKTHGLERR
                     VDA"
     gene            7594..8091
                     /locus_tag="PLS90_09030"
     CDS             7594..8091
                     /locus_tag="PLS90_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPK02587.1"
                     /translation="MLELDAAITCLERAPRLLRELLAPIPAELLRRRRLAGKWTIHEH
                     ACHVATVERDFFFERWRRFQEEARPEFTYFGGESNPDDYYLRMDLNRTLAEFDESRAR
                     VVELARAADADFWSRPAVHPQYTHYTPAIMVRHLMLHDQFHMYRIEELWLTRDEFLSP
                     APAPQ"
     gene            8093..9451
                     /locus_tag="PLS90_09035"
     CDS             8093..9451
                     /locus_tag="PLS90_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK02588.1"
                     /translation="MPPRSPAPLLILALFAAGCVRLPAPVGAPPQWRAAQQPPLPPDQ
                     AEALATAPTLHQALRLALERNPALAAARRRWFAAIQTIPQAASLPDPMLEAGVDLTEP
                     MPMLERGWSFGIRQELPWWRKLWARGELAAREADVMRLEYEIAARDLLIDVKDAWYEL
                     YYLDQAAPITARVEEILRNQALLAYGELRTGRTQLAEAFRAESQAAQLAYDRLLLADE
                     RVARAERLRALLNLPPGAVIGPITAAPIYPVTDDRQELYARAEQWAQRLRISGLRAEQ
                     AEYEAYLARLSRLPDLTLGWMNEANVPAMMGDTMRTALGGMNLPIWQQRNHALIRERE
                     ALAEAARLDAAAELNRLGEAVANAWLRVRTTDRLARLYCEELLPRAQAAMAQAEALFR
                     ADQAAFASLLETTMAWHNFQLAYHRARADHGQAIGMLERALGTTAEARPAGATPDGEG
                     RP"
     gene            9448..10968
                     /locus_tag="PLS90_09040"
     CDS             9448..10968
                     /locus_tag="PLS90_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK02589.1"
                     /translation="MNNRTTCLRLLFCFALLPALALSGCAHRAFSDRLSEYEAQRELP
                     FSARTLAWTAQAAAGELAAPAPASPPPVALADAATSPSEPVREQVLASVLEISPARLD
                     ALRQTIASPAARDAALADRLEWPELALAVAVASPAARAAEERWRATLRQFSQAEHLES
                     LINQYRSFTATLETGAGDPLNRAMTDAFFPYPSTLAFKGEMIREQARMARLEWELALR
                     AALIEAGQAFFQFQYLHRAEATTRENLSLVGDLLAVIEQRYAAGQAGQADLLRMQTLR
                     ERLKNELRDLQSRQRVARAEINALLGRGPEAALGTPASRDLPLNDVGLAELARAALDQ
                     RQEVKLAESRAALRAIAIRMGEIMNRPPASQGASLFEPRMPAAEAAARPAPRPAFAQA
                     EAYLAELRGRLAADEAESAQARATAQARAAAWLEEREIARRAAALLREIVLPQNRSTY
                     EASLSAYSAAELTFVELLDAERALLDARLELDAARRELNQVLIRRAEVTGRVPVGD"
     gene            11110..11586
                     /gene="bcp"
                     /locus_tag="PLS90_09045"
     CDS             11110..11586
                     /gene="bcp"
                     /locus_tag="PLS90_09045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPK02590.1"
                     /translation="MPVEAGQPAPDFTLPSSAGGELTLSSFRGRNIVVLYFYPKDNTS
                     GCTREACAFRDARDELAQLGAVVIGVSPDSLKSHDKFIRNHNLNFPLLADEEKQVCEA
                     YGVWVQKSMYGRKYMGVERSTFIIDREGRIARAWRKVSVAGHDDEVIAAVRELTGA"
BASE COUNT         1874 a         3982 c         4007 g         1909 t
ORIGIN      
        1 gccgtccatc cgcaggaagg ccttcaggtt ggcccccgcg tcgacaacgg cgatgttcat
       61 cgccagcttg agttgttcag ccttcttgcg cgccgcttcc agcacggcct gcgcttgttc
      121 ggcggtaata tccgctccca gtggactcat ggtgggtctc ctgtcgtctt gaaggatcat
      181 cttgggtttg gggacgcgtg cccgcgaact gttcttttac cgcaccggcg atggaagtgc
      241 aagcctccgg cgtgatttcg ccgcaggccg gcccttcgaa ggcttgtcat tccccaaatc
      301 gcaagctgct taaggcttcc atcttcaatg caggcggttc cgacttgtta accgggcaag
      361 tccgcgaggc ggacgaccgg aataaagcgc ggggtgacgg ctgcaggccg gaaccctgcg
      421 tcgccccccg acgaatcaaa cccctgcaag gggtggccgg agttggcctg aattcgtgga
      481 attagaagca ctctgaaggg cgaacgatca accgtcgcat ggaatgatta ttgctccctc
      541 ttcgcgatgt ggatggatct ggaacgaagc cgtgggcttc gatcatcaac ggtgaatttt
      601 ctccgcatcg agcagggagg tgaatcaaca tggccaacgc ggtccaacag gatatgaatg
      661 atgttcgctc ggatcttcgc cagctgcgcg aggatctgga ttcgatcaag gccgacctgg
      721 cgcgcctggc gcacacggcc gtcgactcgg gcaaggacaa ggcccaccgc gccagagaac
      781 gcgcgggcga aaaactggag gacgcctatg aacgcgtccg ccatgagagc gagcgcgcct
      841 tcgattgcgt gcagcactcg gtggaagagc ggccgatcac cagcctgctg accgcgttcg
      901 gcgtcggcat gctgctgggc aagatcatct cggagaaata ggcgatgacc gaactggtcg
      961 aactgttcct ctcggccatc gatgtcatca aggcggagct gcgcaagtcg cgcgagggag
     1021 tctatgagct ggccgtcatc gcggggctgg tggtctgcgc attttttctg ctcgtggtgg
     1081 cgttcggtct gattgtcgcc gggacgttca tcggtttggt caacagcaac gtgcccgtcc
     1141 cgctggcggc gctgtgcacg agcgtcctgt gcatcctgat tgcatggagc ctgatatggt
     1201 ttggtcggga acgagcaaga cggcgctagg cgcgcggcgg gcggagattc ccccggaaaa
     1261 cgaggccgag gcggcgcgga tgcggttgcg cgccgccgcc ggcggcgttc cggtggagac
     1321 ctgcgtccgg cgccgcccgt ggatcagcct ggcgctggcg ctcggggcgg gcgtcgcctg
     1381 cggcgtgtcg cccgcgttgc gccgcaccgc cgtcacgacc gccatcgcgc tcctgcgccg
     1441 gtgcggttga cgggcgaggg gagcgtttgc cgtccgaccg atccggccgg gtgggggatt
     1501 tgccacatgg aacgctgctg gatatgcaac agcgcgccgc gaaataaagg cggcgcggcg
     1561 ccgatcggga cggtggcgtt catcgcccac agcggttttt acgtctggaa ttgaaggttt
     1621 tgcgcatcgg acgccccggc gggccacctc ttcggcgccc gccgcgcccc gctggcatac
     1681 gggctgctct ggtccctggg ctcgggggat tgaaacgctg cgcgccagcg catcccccga
     1741 ccgggcggcc tgagcccgga cgccagccag cgacgttttc catgccttcc caggatcccc
     1801 acacgcgccg ccgatcatcc accggcgcgg cgcctctccg cctgcttttc gtgctggccg
     1861 ccggcctgtt gctgggtggc ctcggcggtt atctgatcgg tctgcgaaag acgccggcgc
     1921 cggcccggca cgccgcggcg caacccccgc cgccgagcgc gccccgcacg ctctacactt
     1981 gcggcatgca tcccgaggtg attagcgagg aaccgggact ctgccccaag tgcgagatga
     2041 aactgacgcc gatggatccg gaccgtgcgc ggctgatcct ggaggcgcgc ggcgagggga
     2101 gcgcgacgcc cgcgccgccg gcgggcgagc gcaacgtcct ctattggcgc tcgccgatgg
     2161 accccgcctt catccgcaac gagccgggtg ccgacgcgat gggtatggac ctcgtcccgg
     2221 tctacgagga cgaagtcgcc gggggaccct cgatccggat tgatccggtc accgaacaga
     2281 acatgggcct gcgctacgac gtcgtccgcg tcgggccgtt gcgcaagacc tttcgcaccg
     2341 tcggcacggt ccagtacgac gagcagagcc tggggaccgt gaccaccaaa gtggagggct
     2401 gggtggagaa ggtgcatgtg caggagacgg gcacgcaggt gcatgccggc gacccgttgt
     2461 tcgatctgta ttccccgcag cttttcagcg cgcaggagga gtacctgacc gcgctgcgcg
     2521 acgtcgaacg cagctcgggc accgcggccg acctggcgcg gacgcggctg cgttcggccg
     2581 aggagcgcct gcgcctgttc gacatccgcg acgagcagat cgatcagttg cgcacgacgg
     2641 gggaagtctc gcgcacgatg acgatccatg ccgatctgac ggggatcgtg atcgaacgca
     2701 aggtgaccga gggcgacatg ctgaccccgg ggatgccggc ctatcgcatc gcggatatct
     2761 ccaccgtctg ggtcatcggc cggatctacg aatccgatct gccgtttgtc cggctcggcc
     2821 aggaggcgct gatgcggctg gactacctgc ccggccgaac ctggcgcggc cgcgtgacct
     2881 acatctatcc ctacctgcct gagggaacgc gcgaggtgcc ggtgcggatg gagtttcata
     2941 atccgggcta tgaactgaag ccggggatgt atgccacgat ccatctggag actacgctgg
     3001 cggccgaggc ggtactggcg ccggcgatgg cggtgatcga caccggcgag cggcagatcg
     3061 cgttcgtcgc gcgcgaaccg ggcaagttcg aaccgcgccg cgtccagacc ggcgcgcgca
     3121 acgaccggga tgaaattcag gtgctggcgg ggctggcgcc gggcgaacgg gtcgtcgtca
     3181 gcgggcagtt cctgctcgac agcgagagcc gcctgcgcga ggcgacgctc aagatgctgc
     3241 agcccggttt ggtgcagacg agcgaggtgc tggaccacgg ggcggagccc gccgcgcggc
     3301 cgggcgccat ggcgtttgaa cgcggaacag acgcgcagcc ggacgcggtt gaccccgtgc
     3361 gctacgtctg cccgatgccg agtcatgccg gcatcctcta cgatcagccg ggggactgcc
     3421 cgctgtgcgc gatgaagctc gtgccggtgc gtgaatccga ccggccgccg gcgtgggtcg
     3481 ccgcgcactg gacctgcccg atgcccgagc atgcccacat cgaggccgaa ggaccgggca
     3541 attgcccgga gtgcggaatg acgctgatcc cggtcatccc gtcggccgaa gccgcaaaaa
     3601 aatccgaatc agtcgaagcg accgcgggcg agtcggccgg catcgcgttg tatgtctgcc
     3661 cgatggagag ccacatgcac atcgtgagcg atcagccggg acgctgtccg gagtgcaaca
     3721 tggtgctggt gccgaccagc gaggtgccgt tcggcaagga aagcgaactg atctggcgcg
     3781 ccctgcacgg cgccggcgac gaaccgacga catcaagcgc gggggcgcgc tgatgaaccg
     3841 cgtcatcgcc cggccaatcg cgcgcatccc ggcaatgttc gacagttggt ctgtctttca
     3901 gcccgtcgtg gaggacccgt tcgagcagga agctcttgtg gagtgcggcg gcagaaagcg
     3961 cgcagcgctt ggcgacgccg ctttggatcg gtcggcatca gcccatgtcg tcggatctaa
     4021 acggtgtcga cgcggtgcgc tgcaacgctc tgccgccaca ctccacaaag gctgcctgga
     4081 tctcgctgct caggtcaagc attggaattc gacgcgtgtt tccattctcg ccattcggat
     4141 tgtgaacggg catgattcgc cggattattg aattctcgct ggccaataaa ttcatcatcc
     4201 tgctgatgat gctggcgatt gtcgccggcg gaattcacgc ggcgaagaat atccgtctcg
     4261 acgccatccc cgacctctcc gacgtgcagg tcattatcct caccgaatac gccgggcagg
     4321 cgccgcggat cgtcgaggac caggtcacct atccgatcac gaccaagatg ctggcggtgc
     4381 cgtacgccaa ggtcgtccgc ggctattcgt tttacaatta ttccttcgtc tacgtgattt
     4441 tcgaggatgg gaccgacatg tactgggcgc gctcgcgcgt cctggagtac ctctcccagc
     4501 ttcagtccca gctccccgac ggggtgtcgc cgcagctcgg gcccgacgcg accggcgtcg
     4561 gctgggcgtt tcaatacacg ctgaccagcg acgcgcgcga cctggcccag cttcgctcgc
     4621 tgcaggactg gttcctcaag tacgagctga cttcgatcga cggcgtcgcc gaagtggcca
     4681 gcctgggcgg cttcgagcgc cagtatcagg tgacggtcga gccggaaaaa ctgcgggcgt
     4741 atgacctgtc aatcaccgcg gtgcgcgatg cgatccgcaa gtcgaacaac gaggtcggcg
     4801 gccggacgat cgagatggcc gagaccgact acatgctgcg cggcatgggc tacctcggca
     4861 cgctatcgcc cgaagagcag cgcgcggccg aggcgcgcgg catcgcgatc gacctcgcgc
     4921 gcatggataa gacgatcgac gagctggagg cggtggcgct gggcgtccgc gccgacggca
     4981 cgccgatcct gctgcgcgac gtgggccggg tggcggcggg ccccgagatg cgccgcggcg
     5041 tgctggaatg gaacggcaag ggcgaggccg tcggcggaat cgtcgttgtc cgttacggcg
     5101 ccgacacgct ggcgacgatc gcccgcgtgc gcgagcggct cgccgagatt cggcgctcgc
     5161 tgcccgagga cgtccggatt cacgtcggct acgaccggac ggcgctgatc gagcgcgcga
     5221 tcgccacgct gcgccacacg ctcatcgagg aatccgtcat cgtttccatt atcattatcc
     5281 tctttctatt gcattttcgc agcgcgctgg tgccgatcct gaccctcccc ctggcggttc
     5341 tcatcagctt cgtcatcatg cactaccagg ggctcggcgc caatatcatg agcctgggcg
     5401 ggatcgcgat cgccatcggc gcgatggtcg acgcctcgat catcgtcgtc gaaaacgtcc
     5461 acaagcatta cgaaacgaaa cgcgaggacc agccgcactg ggaactcgtg cgcgaggcgt
     5521 gcgtggaggt cggcccgacg ctgtttttcg ccttgctggt gattacggtc agcttcatgc
     5581 cgatcttcac actcgaggcc caggaagggc gcatgttccg gccgctggcg ttcaccaaga
     5641 cctacgcgat ggcggcctcg gcgtttctgg cggtcacggt ctcgccggtg ctggccgggt
     5701 ttttcatccg gcgcgacgta ttgccgccgc gctggtcgcg ccgccgccgc cggctggcgc
     5761 tgctgctcac cccgccgctc gtcttcctgc tcctctggct cggcacgcgc cgggcgggcg
     5821 ggggcgcgtc gggctccgcg ctcgggcttt ccgggtggac atgggtggcg ctcctcgtcg
     5881 cgctcgcgac gttcctggcg ctgctgccgc aaacgatgca cccggaggag cgcaacccga
     5941 tcagccgcgg cctcgtccgc gcgtatcagc cgtttctggc gctgctgatc cgttcgcgcg
     6001 cggtctgctg ggccatcgtc gtcgcggcgc tcctctccgc gctgacgatc ttcttcccct
     6061 atctccatct gggccgcgaa ttcatgccgc cgctgtatga gggcgacctg ctttacatgc
     6121 cgaccacgct gcccggcatc agcgcgacga aggcccgcga actcctgcag cagaccgaca
     6181 agatcatcgc cgccttcccc gaggtggaat cggtctacgg caagatcggg cgggccgaaa
     6241 cggcgaccga ccccgccccg atgatgatga tcgagacgac gatccggctg aaggacgaag
     6301 cacagtggcc ggcggtggag atccgcgacg acgccggccg cgtcgttgac cgccggccgc
     6361 gcacgtcgga ggaactgacc gacgccctca accgggagat caagttcccc ggcctgacca
     6421 acgcctggac gatgccgatc aagacccgga tcgacatgct ctcgaccggc atcaagaccc
     6481 ccgtcggcat caagatcggc ggctcggacc tggcgacgct ggagcgcatc ggcgagcagg
     6541 tcgaggcggt cgtccgcgat gtccccggca cgctgagcgt ctatgccgaa cgcgtcatgg
     6601 gcgatcgcta cgtgaacttc atccccgacc gccaggcgct ccagcgctat ggcctgacgg
     6661 tcgccgacgt ccaggaagtc ttcatcaccg cgatcggcgg gatggcgatc gccaacacgg
     6721 tcgaggggct ggagcgctat tcgatcaacc tgcgctatcc gcgcgagctg cgcgacaaca
     6781 tcgatgaact gaagcgcgtc gtcgtcccca cgccgcaggg ttttcacatc ccgctcggcg
     6841 atctgggcgt gttccagatc gagaccgggc cggccggcat caagagcgag cagagccgcc
     6901 ccaacgcgtg ggtctatgtc gacctggacg acatcgacgt ggggacctac gtggagcacg
     6961 cgcgcgcggc ggtcgatgcc gcaatcgccg acgggcggat cgtcctgccc gccggctatt
     7021 cgatccactg gtcgggccag tatgaataca tggtgcgcgc gcgggcccgg ctgcgggcgg
     7081 tcgtcccgct cacgctgctc atcattctcg tcctcattta cctgagcacg ggcagcgcgt
     7141 ttcaaacttt cgtcgtcatg gcgtgcctgc cgttcagcct gctcggcgcg ttctggatgc
     7201 tctattggct cgattacaac ctgtcggtgg cggtctgggt cggcatcatc gcgctggccg
     7261 gcctggatgc cgagaccagc caggtgatgc tgctctacct cgacctggcc tgggacaaac
     7321 tcaagcgctc cggggcgcgg ctggactggg cggccgcgcg cgcggcggtg ctcgaaggag
     7381 cggccacgcg cctgcgcccc aaggcgatga cggtcggctc gaccctcttc ggcttgctcc
     7441 cgatcatgtg ggcctcgggc gccggcgccg acacgatgaa acgcatcgcc gtcccgatga
     7501 tcggcggcat gatcaccagt tttattctgg agatggccgt catcccggtg gtgtattatc
     7561 tgtggaaaac ccatggattg gagagacgtg tcgatgcttg aactggatgc cgccatcacc
     7621 tgcctggagc gcgccccgcg gctgctgcgc gaactgctgg ccccgatccc cgccgagctg
     7681 ctgcgccggc ggcgcctcgc gggcaaatgg acgatccatg aacacgcctg tcatgtcgcg
     7741 acggtcgagc gcgatttctt cttcgagcgc tggcgccggt ttcaggaaga agcgcgcccg
     7801 gaattcacct acttcggcgg cgagtcgaat cccgacgatt actatctgcg gatggacctg
     7861 aaccgaacgc tggctgagtt cgacgaatcg cgcgcccgcg tcgtcgaact ggcgcgcgcg
     7921 gccgacgccg acttctggtc gcggcccgcc gtccatccgc aatacacgca ctacaccccc
     7981 gcgatcatgg tccgccacct gatgctgcat gatcagtttc acatgtaccg catcgaggaa
     8041 ttgtggctga cgcgggacga attcctctcg cccgcacccg ctccgcagta acatgccgcc
     8101 aagaagccca gcgccgctgc tcatcctcgc cctcttcgcg gcgggatgcg tgcggctgcc
     8161 cgctccggtc ggcgctccgc cgcagtggcg cgccgcgcag cagccgccgt tgccgccgga
     8221 tcaggccgag gcgctggcga ccgcgccgac gctgcaccag gcgctgcggc tggcgctgga
     8281 acgcaacccg gcgctggccg ccgcgcgccg gcgctggttc gccgcgattc agacgatccc
     8341 gcaagccgcc agcctgcccg atccgatgct cgaggccggc gtggatctga ccgagccgat
     8401 gccgatgctc gaacgcggct ggagtttcgg catccggcag gagctcccct ggtggcgcaa
     8461 gctgtgggcc cgcggcgagc tggccgcccg cgaagcggac gtgatgcgcc tcgaatacga
     8521 gatcgccgcg cgcgacctgc tgatcgacgt caaggacgcc tggtacgaac tgtattacct
     8581 cgatcaggcc gcgccgatca cggcgcgcgt cgaggagatc ctgcgcaacc aggccttgct
     8641 ggcgtatggc gagttgcgga ccggacgcac gcagcttgcc gaggccttcc gcgccgaaag
     8701 ccaggcggcg cagttggcct acgatcgcct gctgctggcc gacgagcgcg tcgcgcgcgc
     8761 cgagcggctc cgcgccctgc tcaatctgcc gcccggcgcc gtcatcgggc caatcacggc
     8821 ggcgccgatc tatccggtca ccgacgatcg ccaggaactg tacgcccgcg ccgagcagtg
     8881 ggcccagcgc ctgcgcatca gcggactgcg cgccgagcaa gctgaatacg aggcctatct
     8941 ggcgcggctg agccgcctcc ccgatctgac gctgggctgg atgaacgagg ccaacgtgcc
     9001 ggcgatgatg ggcgacacga tgcgaaccgc gctcggcggt atgaatctgc ccatctggca
     9061 gcagcgcaac cacgcgctga tccgcgagcg cgaagcgctg gccgaggccg cccggctgga
     9121 cgccgccgcc gaactgaacc ggctcgggga agcggtcgcc aacgcctggc tgcgcgtgcg
     9181 caccaccgac cggctcgccc ggctgtactg cgaggaactg ttgccccggg cgcaggcggc
     9241 gatggcgcag gccgaggcgc tctttcgggc cgatcaggcg gccttcgcct cgctgctcga
     9301 aaccacgatg gcctggcaca actttcaact cgcctaccat cgcgcgcggg ccgatcacgg
     9361 ccaggcgatc gggatgctgg aacgggcgct gggaacgacg gcggaggccc ggccggccgg
     9421 cgcgacgccc gacggggagg gccggccgtg aacaatcgga ccacctgcct gcggttgctc
     9481 ttctgcttcg cgctgctgcc ggccctggcg ctcagcggct gcgcccaccg ggcgttcagc
     9541 gaccggttga gcgaatatga agcgcagcgc gagttgccgt tcagcgcgcg gacgctggct
     9601 tggacggccc aggccgcggc cggagagctc gcggcccccg ccccggcgag cccgccgccg
     9661 gttgcgctgg ccgacgccgc gaccagcccc tccgagccgg tgcgcgagca ggtgctggcg
     9721 tcggtcctgg aaatttcgcc ggcccggctc gacgccctgc ggcaaacgat cgcgtcgccc
     9781 gccgcgcgcg acgcggcgct ggccgaccgg ctggaatggc ccgaactggc tctggcggtt
     9841 gccgtcgcca gccccgccgc ccgtgcggcg gaggaacgct ggcgcgcgac gctccgccag
     9901 ttcagccagg ccgagcacct cgaatcgctg atcaatcaat accgttcgtt cacggcgacg
     9961 ctcgagactg gcgcgggcga tccgctcaac cgcgcgatga cggacgcctt ctttccctac
    10021 ccctccacgc tcgcgttcaa gggtgagatg atccgcgagc aggcgcgcat ggcccggctc
    10081 gaatgggaac tggcgctgcg cgcggcgctg atcgaggcgg ggcaggcttt ttttcaattt
    10141 cagtatctcc accgcgccga ggcgaccacg cgcgagaatc tctcgctcgt cggcgacctg
    10201 ctggcggtca tcgagcagcg ctacgcggcg ggccaggccg gccaggcgga cctgctgcgg
    10261 atgcagacgc tgcgcgagcg cttgaagaac gaactgcggg acctgcaatc gcggcaacgg
    10321 gtcgcccgcg ccgagatcaa cgccctgctg gggcgcggcc cggaggcggc gctgggcacg
    10381 cccgcgtcgc gggatctgcc gctgaacgac gtcgggttgg cggagctcgc gcgcgccgcg
    10441 ctggaccagc gccaggaggt caagctggcc gagtcgcggg cggcgctgcg cgccatcgcg
    10501 atccgtatgg gcgagatcat gaatcggccg ccggccagtc agggggcaag cctgttcgaa
    10561 ccgcggatgc ccgcggcgga ggcggcggcc cggcccgcgc cgcgcccggc ctttgcccag
    10621 gccgaggcgt acctggccga actgcgcggc cggctcgccg ccgacgaggc ggagagcgcg
    10681 caggcgcgcg ccaccgcgca ggcccgcgcc gccgcctggc tggaggaacg cgagatcgcc
    10741 cggcgcgcgg cggccctcct ccgcgagatc gtcctcccgc agaatcgctc gacgtatgag
    10801 gcgagcctgt cagcctacag cgccgccgag ctgacgtttg tcgagctgct cgatgccgag
    10861 cgcgcgctgc tcgacgcgcg gctggaactg gacgccgccc gccgcgagct gaaccaggtc
    10921 ctcatccgca gggccgaggt gaccggccgc gtgccggtgg gggattgaac gaccgcgggc
    10981 ggccgcgttc gccgctccgg cgacgcttgc ggggctcggc gcgttccgat aaattgagac
    11041 cgcggccgcg accggcgagc cccgccaacc agccacacgc ttgacgtcag accaacggga
    11101 gcgcgaacca tgcccgtcga agcaggccag ccggcccccg atttcactct tccctccagc
    11161 gcgggcgggg aactgaccct ctcctccttc cgcggccgga acatcgtcgt gctctacttc
    11221 tatccgaagg acaacacgtc gggctgcacg cgcgaggcgt gcgcgttccg cgacgcgcgc
    11281 gacgagctgg cgcagctggg cgccgtcgtc atcggcgtca gccccgattc gctcaagtcg
    11341 cacgacaagt tcatccgcaa ccacaacttg aatttcccgc tgctggccga cgaggaaaag
    11401 caggtctgcg aggcctatgg cgtgtgggtg cagaagagca tgtacgggcg caaatacatg
    11461 ggggtcgaac gctcgacgtt catcatcgac cgcgagggca gaatcgcgcg cgcctggcgc
    11521 aaggtcagcg tcgccgggca cgacgatgag gtgatcgcgg ccgtgcgcga gctgaccggc
    11581 gcgtagccgc cgcgcctgat tggttcccac ggtgggaatg gcgtggtctc agttcttgag
    11641 gcatcccggc agggatgcca gagggtagcc ggtggttgag cgcagcgaca ccaccggtcc
    11701 cccggcaacc ccaaacgccc gcatcccgga gggatgccag caggtagccg gtggttgagc
    11761 gcagcgacac ca
//
LOCUS       DAOPCO010000108        11763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5378_length_11763_cov_6.079433, whole genome shotgun sequence.
ACCESSION   DAOPCO010000108 DAOPCO010000000
VERSION     DAOPCO010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5378_length_11763_cov_6.079433"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1025
                     /locus_tag="PLS90_09050"
     CDS             288..1025
                     /locus_tag="PLS90_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK02591.1"
                     /translation="MRIAVISDIHGNLPALEAVVGDLARRGADCVVNLGDALSGPLLP
                     GETARFLIEQDWLHLAGNHERQLLASTANEQGDADEFARACLSPRELEWVASLKPATA
                     FSADVLLCHGTPTTDAQYLLETVEPAGVRLAAPAEVDLRLNGVAAAVIACGHAHVPRS
                     IRNSRGQLLVNPGSVGLPAYRARRPHPHTIETGSPDARYAIVERRNGGWMATHFAVPY
                     AHQSMAELARSRGNAMWARALATGYVS"
     gene            1217..>2196
                     /locus_tag="PLS90_09055"
     CDS             1217..>2196
                     /locus_tag="PLS90_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02592.1"
                     /translation="MNRIIRLSMIALLAILAHRQAAAQWPVDLTTDPGLTVTSNQAGH
                     PTFPLSNLTDKGDTTSFSSLWLEETANGIQNTAEIIFEWASPVHIEYVEVANFYENMR
                     SYSLSTWDGSQWVTNHTATDLPNQIYRIDAFRKGELNGADFPDQTTRLRINITAVWVE
                     GQGARLSEVTVLDALPPTPDRPADYNLAHIAGTSTGEFRRINQQDPFFDVTGDGLVDL
                     ALPSATGSRIYLQAPTEPRFAADSYLELSKDGAQYPSAMFYGHLNNDAFPDVGILQGH
                     TTAVVETQIFRGTSSGVEGAPFIKFSPPTSRSVVTAHYGTSGDVNGDGRQ"
     assembly_gap    2197..2296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2297..2764
                     /locus_tag="PLS90_09060"
     CDS             <2297..2764
                     /locus_tag="PLS90_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK02593.1"
                     /translation="SGPKSDIAVGDVDGDGGLDLVASGGTGIAVWTNQSTLWATGQLG
                     VPPDFELELAWNILGCALADINSDGRDDLVVGGDLGYVFVLLANASGPLFNTGNSRVY
                     QVEGFFAVGDVVVQDLDNNNNPDLVAIEQTWGNGAVVMDVGILPNAVSNWAIY"
     gene            2798..3730
                     /locus_tag="PLS90_09065"
     CDS             2798..3730
                     /locus_tag="PLS90_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02594.1"
                     /translation="MRKLLMAVMLIGSLELPAAAQTHLEDTATTVISNRGQHATYPYA
                     NVRDDLIDVPGNDPFKMWLENTDDGIQGDGWLEFQWDSPVHIEYVAVHAHYYDIKTYS
                     VETWNGSSYVVHNTLTDEPLVLYRMDEHRNGDMSGPDFPAATTRLRINVAEMWTPSQG
                     LRISEVEILDQNLLPEVNVEPVALDFTEFSGYEVNIQAGETTKTQQITVENTGDAQLR
                     VDDLVISGANAAAFSVDPGDYPFGGQALQDRMPINHIAPGEAKVLNVRFHPNVGSNTF
                     GMIGLLAIENNDQTVNVALEGDAVPAELSDFLLE"
     gene            3856..4494
                     /locus_tag="PLS90_09070"
     CDS             3856..4494
                     /locus_tag="PLS90_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02595.1"
                     /translation="MTKRTPGFTLIELLIVVAIIAILAAIAVPNFLEAQTRARISAVK
                     ADQRTVDIALNSYRVDYNTIPYTDILGVIFRLTTPVAHLSSPLQAAFFDLHDKYYGLG
                     TAYGNSIVELYGMIPFGVYNREWYNDVYKVLNTAPGTSTASGPWIYETNFQVFAQTGE
                     YMMLSQGPHGGNAYEPYSSPPGYGWIWLSYDPTNGTISNGNIIRLDGRGIQN"
     gene            4683..7052
                     /locus_tag="PLS90_09075"
     CDS             4683..7052
                     /locus_tag="PLS90_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02596.1"
                     /translation="MNPIHPGPPFAIRQAQNTITLLREGQPLAEISLPFDCQPLEPDR
                     QPEFVELFGAGKRLRWAFEPQAGLRAELWLSVYNDLPFVLLGGRLAATGPAPVLVEDI
                     TTCDIVLRRDSHAQSRFLINSGSQGFSGSLPLDAAEVAQSAAEAVAASSETIDLVLTC
                     STLCAFRGATPDDCLVAGFITVRRAEAFFGRRREGDVFRIQPRIAYDQVQLDRGEALT
                     LETMMLVTDRTAGRALETWADAVAAVLRPQFSRSMLGLYNTWYAYWTPTSDHGDAALW
                     VRGTQELVESGLIHYGIAGSFAGVHHNRAAFGEPEPWPGHFPDGFGPPVREIRARGGV
                     ACGGVFWGHVSKCSSLFEQHPEFMIKDADGQPLEQSPVSWGACPHPFYDLDLTDPEAF
                     ECLRRVAPRPFAELGIKYFLMDFMGLASRRSKGARPADPKVCWPVEAERRQLAMFKEE
                     LGEGAMIGVYTSPTNRFAGLVDRVRLSKDAGVITAVSQLDDAGRGNLAHKTNADFRWE
                     WTQDVARNMAAGYFYNEKLWINDPDPISVGIDDEPQTLEEARVRIMICACSGGFPTVG
                     EPLSRMHPNRMAMLKQALPAYGHAYRCLDLMDQTVPSIHHLHVPQPGGPYELLALFNW
                     NDEPRRFDLDLEAMDLFGAHHGFDFWEQAPLGVLAGRFNHTVPPRAARLLRLTPLAAR
                     PQLIGTDRHVTVGALEVGGLCWDAASANLRGVARRPLPETGTLFFAVPEDWSLLDADG
                     LNLRQEDGIVRLELEFTPTGAPWRLAFAPAAGAVGASQAPAQRSLSVSN"
     gene            7133..7966
                     /locus_tag="PLS90_09080"
     CDS             7133..7966
                     /locus_tag="PLS90_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK02597.1"
                     /translation="MIVTNEHRQMYREEGYFILEKAIDPATLRQLQDESARLKHCFDE
                     KMRESGTDVWDISHYGKRYFISSYHTQSPILERYLFSELMAEICRATLGPEVFLFQEQ
                     FVIKGAEVGMKFSWHQDGGWFYPDCPPYVTVWAALDDMTEENGTIYVLPWSHMDRRGW
                     IEHKLCPQTNDRVGYDGPDPGIPVFVPAGSIVVFSNQTLHRSGPNVSPNLRRAYLAQY
                     SCAPILNRHFIYADYADPAVTPDSLPFFARRFLENWEINRRDTYPLRQPYHVEAMKAP
                     A"
     gene            complement(8419..8505)
                     /locus_tag="PLS90_09085"
     tRNA            complement(8419..8505)
                     /locus_tag="PLS90_09085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8469..8471),aa:Leu,seq:caa)
     gene            complement(8703..10385)
                     /locus_tag="PLS90_09090"
     CDS             complement(8703..10385)
                     /locus_tag="PLS90_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK02598.1"
                     /translation="MITRHPHSRTLVIRTYGNLDALQVEHYRQQIRLLVDNGYRSLIF
                     DLEETPFINSSALGLLVEFHNTMRRLNGSVRLVNCSEQVVWMLQQTRLDVLLLVDEPP
                     RELVPEPTLESLHSLMVEEVLLLSHINQFTEQAFRENDPVEIGRMILRTVMQALKSAR
                     GIVFLLGEDGRVMRLIHHEGAPLACPAEQIREVHLGHAKLEDAILKSQDVSWHEFSGF
                     PAPEEWLFRKIGFASMLAAPIASGERCFGLLAVEGSEETAHALRAVRPLLRTLTNICG
                     LAIEKAHLLSEVRARNAELEHAVARLKQFHQSLHGAGRLAAMGTVVSGLGHQLNNKMV
                     PLVGYTQLLMMDKNLGEQQKQRMESIHKTTQEIKEIVEKLVKVARPSRADAGQCDLGL
                     VIQTALDLLSEPIGKNRISIRVNLGQEPVALGGDPTALLQVLVAILHRACHSFAPDAE
                     ESRVWIIGSVAEGNLRLCIEDNGDDLDEFDTGEWADPLVPFQAMKHGRLFNYTIPRSL
                     LSRMHGKLQLEPREGGGKRVVIDLPVGQDAPPAAGAAARPDAGAPRSSRPAN"
     gene            complement(10576..11589)
                     /locus_tag="PLS90_09095"
     CDS             complement(10576..11589)
                     /locus_tag="PLS90_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPK02599.1"
                     /translation="MPAQPKQPAQPDNLVVEIAQYLERELAAVDRVLHDILDSQSSLV
                     REVGEYICLAGGKKLRPMITLLAARAYNAPSPAPVQLAAAMEAVHVATLMHDDVIDKA
                     PMRRGRPSVNARWGDDVAILMADYLYAAAFELSLNYLDHEPLRLICQVTRRMCEGEMF
                     QIEKRGRWLEPEDYIHLITCKTACLFSACAAVGGIAGGLDPVTIARLSAFGLEFGLAF
                     QITDDALDLTATDALWGKPVGIDMATGKQTLPLILALRQATDAERRTIESILENGGSV
                     AAANGEMLAILERYGCIDEALETARRFARQAVAHLDGLTVYDEVAAEYMKILPDYVIT
                     RQY"
BASE COUNT         2070 a         3921 c         3580 g         2092 t
ORIGIN      
        1 cgcatcttgc tatacatagc aaatcgtata tcattcagaa taaccctgcg tcaacgggaa
       61 tcgtggttga acctggttcc gcctgcgggg ctgtttgatg cttttctggc ggagattgtg
      121 gcaaagcccg ccacagttta acgtcaagct caatccagca atccggtccg tgaaattcat
      181 gatgaaaaac acgttgacta cttcttgcaa tcactgctat cttccatgca gacttagata
      241 gcattcatag caaaaatgat cccgaggtct ggattcgcca cccgcccatg cgcattgcgg
      301 tgatctccga tattcacggg aacctgcccg ccctggaggc cgttgtcggc gacctcgccc
      361 gccgcggcgc cgactgcgtc gtcaacctcg gcgacgccct ttcggggccc ttgttgcccg
      421 gcgagaccgc gcgctttctg atcgagcagg actggctgca cctggcgggc aatcacgagc
      481 gccaactgct ggcgagcacg gcgaatgaac agggcgacgc cgatgaattc gcgcgcgcct
      541 gcctttcgcc gcgcgagctc gagtgggtcg cctcgctcaa accggcgacg gccttctccg
      601 ccgacgtcct gctgtgccac gggacgccga cgaccgatgc gcaatacctg ctggaaacgg
      661 tggagcccgc gggggtgcgc ctcgccgcgc cggcggaggt cgacctgcgc ctgaatggcg
      721 tggcggccgc cgtgattgcc tgcgggcacg cgcacgtgcc gcgctcgatc cgcaattccc
      781 gcggccagct cctggtcaat ccgggcagcg tggggctgcc ggcctatcgc gcccgccggc
      841 ctcacccgca cacgatcgag accggctcgc ccgacgcccg ctatgcgatc gtcgagcgcc
      901 gcaacggcgg ctggatggcc acgcatttcg ccgtgcccta cgcgcaccag tcgatggcgg
      961 agctggcgcg ttcgcgcggc aacgcgatgt gggcgcgtgc cctggcaacg ggctacgtgt
     1021 cgtgagcgcg cgcgggtcgt cgcgcgggcg ccgccatgcg gcgattcatt cggttcgctg
     1081 tttatacgag gaaaggaggt cgctggtgtc ccaacgcaag acagccgtcg aagtcggtct
     1141 gtcacgggcg agcctggccg tcgccaggat gcgtccccat taagccaatc tgaccgccaa
     1201 ccaggaggac ctctgaatga accgcatcat ccgattatcc atgatcgcgc tgctcgcgat
     1261 cctcgcgcac cggcaggccg ccgcgcagtg gccggtcgac ctcacgaccg atccgggcct
     1321 gacggtcact tcgaaccagg cgggccatcc gactttcccg ctttccaatc tgacggacaa
     1381 gggagacacc acttcgttca gcagcctatg gctggaggaa acggccaacg ggattcagaa
     1441 caccgccgag atcatctttg aatgggcgtc gccggtccac atcgaatacg tcgaagtcgc
     1501 caatttctac gagaacatgc gcagctattc gttatcgacc tgggatggct cgcaatgggt
     1561 gaccaaccat acggcgacgg atctgcccaa tcagatctat cgcatcgacg cgttccgcaa
     1621 gggagaactg aacggggcgg atttcccaga ccagacgacc cggctgcgca tcaacataac
     1681 ggcagtgtgg gtcgaggggc agggggcgcg tctgtccgag gtgaccgtgc tggacgcgct
     1741 gccgcccacg ccggatcggc cggcggacta taacctcgcg cacatcgccg gcacctccac
     1801 cggcgaattc cgccggatca accagcagga tcccttcttt gatgtgacgg gagatgggct
     1861 ggtggatctg gcgctgccga gcgcgacggg gtcgcggatc tatcttcagg cgccgaccga
     1921 accgcggttc gccgccgaca gctatctcga attgtccaag gatggagccc aatacccctc
     1981 cgccatgttc tatggccacc tgaataacga cgcgttcccc gacgtcggca tcctgcaggg
     2041 ccataccacc gccgtggtgg agacgcagat cttccgcggc accagttcgg gggtggaagg
     2101 tgcccccttc atcaagttct cgccgccaac ctcccgctcc gtcgtgacgg cgcactatgg
     2161 cacgagcggc gatgtgaacg gcgatgggcg ccaggannnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnntccg gccccaagtc ggatatcgcg gtgggcgatg tcgacggcga
     2341 tggcggcctc gatctggttg cgtccggcgg gaccggcatc gccgtctgga ccaatcagtc
     2401 aaccttgtgg gcgaccggcc agttgggcgt tccgcccgat tttgaactgg agctggcctg
     2461 gaatatcctc ggctgcgccc tggccgacat caactccgac ggccgcgatg atctcgtcgt
     2521 cggcggcgat ctcggctacg tcttcgtcct cctggccaat gccagcggtc cgctgttcaa
     2581 caccggcaat tcgcgcgttt accaggtcga ggggttcttt gccgtgggcg acgtcgtcgt
     2641 ccaggacctc gataacaaca ataaccccga cctggtggcg atcgagcaga cgtggggcaa
     2701 tggagcggtt gtgatggatg ttggcattct gccgaacgcc gtctccaatt gggcgattta
     2761 ctaatctttg aagcgggagc gaaaggagcg caacaccatg cggaaactgc ttatggctgt
     2821 gatgttgatc ggatcgctgg aattgcccgc ggcggcacag acgcatctcg aggacaccgc
     2881 cacgaccgtg atctccaacc ggggccaaca tgccacctat ccctacgcca acgtgcgcga
     2941 cgacctgatc gatgtccccg ggaacgaccc gtttaaaatg tggcttgaaa acaccgacga
     3001 cggcattcag ggcgacggct ggcttgaatt ccaatgggat tctcccgttc acattgaata
     3061 cgtggccgtt cacgcccatt actacgatat caaaacctac agcgtggaaa cgtggaatgg
     3121 ctcctcctac gtggtgcaca acaccttgac cgatgagccc cttgtgctct accgcatgga
     3181 tgagcatcgc aacggcgaca tgagcggacc cgattttccc gccgccacca cccggctgcg
     3241 gatcaacgtg gccgaaatgt ggacgcccag ccagggcctg cgcatttccg aggtggagat
     3301 cctcgaccag aacctcctgc cggaagtcaa tgtggaaccc gttgcgctgg actttaccga
     3361 gttcagtggc tacgaggtga acatccaggc cggcgaaacg acgaagaccc agcagatcac
     3421 ggtcgagaat accggcgacg cccagctccg cgtagacgat ctggtcatct ccggcgccaa
     3481 cgccgcggcc ttttccgtcg accccggcga ctatccgttc ggcgggcagg cgctgcagga
     3541 tcgcatgccg atcaatcata tcgcgccggg cgaagccaag gtgctcaatg tccgtttcca
     3601 ccccaacgtc ggatcgaaca cgttcggcat gatcgggctg ctggccatcg agaacaatga
     3661 tcagaccgtc aacgtcgccc tggagggcga tgccgtgccc gccgagttga gcgatttcct
     3721 gctggaatga gtggtcttcc aacagcaatc ccaacttccg tcggggaaat caacaccagg
     3781 ctgccatacg ggagccgaac ggcccatacc cgttcggctc ccgcaattcg caaaacaaca
     3841 tcgaggtgaa cggagatgac gaaacgaacc cctggattca cgctcatcga gcttctgatc
     3901 gtggtggcga tcatcgccat cctggcggcg atcgccgtgc cgaactttct ggaggcgcag
     3961 acgcgcgccc gcatctccgc ggtcaaggcc gaccagcgga ccgtcgacat tgcgctcaac
     4021 agctaccgcg tcgattacaa cacgattccc tacaccgata tcctcggcgt catcttccgg
     4081 ctgacgacgc cggtggcgca tctgtccagc ccgttgcagg ccgccttctt cgacctgcac
     4141 gacaagtact atgggctggg caccgcctat ggcaattcga ttgtcgaact gtatgggatg
     4201 attccgttcg gcgtctacaa ccgcgagtgg tacaatgacg tctataaggt gctgaatacc
     4261 gcccccggca cgtcgacggc cagcggcccc tggatctatg aaacaaactt tcaggtcttc
     4321 gcccaaacgg gggaatacat gatgctttcg caaggcccgc acggcggcaa tgcctatgaa
     4381 ccctattctt cgccgcccgg ctatggctgg atctggttgt cgtacgatcc gaccaacggc
     4441 accatttcca acggaaacat catccgtctg gacggaaggg gcatccagaa ttgaggcgcg
     4501 accgtcgcgt cgcgcgacca ttcgttgagc ttcactctgg aaatcgacat gtggggggct
     4561 cggcgcgccg cctccgattt ctccccgttc ccgaatttca atgaaattat tccattggaa
     4621 atagcagatg aatctcgccg gctttcggcg ccccctcatc cctcagtata aaggccagtc
     4681 gcatgaatcc aatccatccc ggccctccgt tcgccatccg gcaggcgcaa aacacaatca
     4741 cgctgctgcg cgagggccag ccgctggcgg agatctcgct tccctttgac tgccagccgc
     4801 tcgaaccgga ccgccaaccc gaatttgtcg agctgttcgg cgcgggcaag cgcctgcgct
     4861 gggcgttcga gccccaggcc ggactccgcg ccgaactctg gctgtcggtc tacaacgacc
     4921 tgccgttcgt cctgctcggc ggccggctcg cggcgaccgg gccggcgccc gtgctggtcg
     4981 aagacatcac gacgtgcgac atcgtgctgc gccgcgactc ccacgcccag tcgcgctttc
     5041 tgatcaactc cgggtcgcag gggttcagcg ggtcgctgcc gctcgacgcg gcggaggtcg
     5101 cccaaagcgc cgccgaggcg gtggcggctt caagcgaaac catcgatctg gtccttacct
     5161 gctcgacgct ctgcgcattt cgcggcgcga cgcccgatga ttgcctcgtt gccggattca
     5221 tcacggttcg ccgcgcggag gcgttctttg gccgccggcg cgaaggcgac gtctttcgca
     5281 tccaaccgcg gattgcctat gatcaggtgc aactcgatcg gggcgaagcg ctgaccctcg
     5341 aaaccatgat gcttgtcacg gaccgcaccg ctgggcgggc cctggaaacc tgggccgatg
     5401 ccgtcgcggc cgtcttgcgt ccccaattca gccgctcgat gctcggactg tacaatacct
     5461 ggtatgccta ctggaccccg acgagcgatc acggcgacgc cgcgctctgg gtccgcggca
     5521 cgcaggaact ggttgaatcc ggcttgatcc attacggcat tgccggctcg tttgccggcg
     5581 tgcatcacaa ccgggcggcg ttcggcgagc ccgaaccatg gcccgggcat ttccccgacg
     5641 gcttcggccc tcccgtccgc gagattcgcg cgcgcggcgg cgtcgcctgc ggcggcgtgt
     5701 tctggggtca tgtcagcaag tgcagctcgc tgttcgagca gcatcccgaa ttcatgatca
     5761 aggacgccga cgggcagccg ctcgaacaga gcccggtgtc gtggggcgcc tgcccgcacc
     5821 cattttatga tctggatctg accgatcccg aggcattcga atgcctgcgc cgcgtcgcgc
     5881 cgcgaccgtt cgccgaactg gggatcaaat actttctcat ggatttcatg ggtcttgcgt
     5941 cgcgccgctc caagggcgcc cggcccgccg accccaaggt gtgctggccg gtggaggccg
     6001 aacgccgcca gctcgcaatg ttcaaggagg aactgggcga gggcgcgatg atcggcgtct
     6061 acaccagccc gaccaatcgc ttcgccggcc tggtcgaccg ggtccgcctg tcgaaggacg
     6121 cgggcgtcat cacggccgtc tcgcagcttg acgacgccgg ccgcggcaac ctggcgcata
     6181 agaccaatgc cgattttcgc tgggaatgga cgcaagacgt cgcgcgcaac atggccgccg
     6241 gctatttcta caatgaaaag ctctggatca acgaccccga tccgatttcc gtcggcatcg
     6301 acgacgagcc ccagaccctc gaagaggccc gcgtccgcat catgatctgc gcctgttcgg
     6361 gcggctttcc gactgtcggt gaaccgctct cgcgcatgca tcccaaccgc atggcgatgc
     6421 ttaagcaggc gctgccggct tacggccacg cctaccgctg cctcgatctg atggaccaga
     6481 ccgtgccctc cattcatcat ctccacgtgc cgcaaccggg cggtccgtat gaactgctgg
     6541 ccctgttcaa ctggaacgat gagccgcgcc ggttcgacct ggatctggag gcgatggacc
     6601 tgttcggcgc gcaccacgga ttcgatttct gggaacaggc accgctcggc gtcctcgccg
     6661 gccggttcaa ccacacggtt ccgccgcgcg ccgcccgcct gctccgcctg acgccgctgg
     6721 ccgcgcgccc gcaactgatc ggcaccgacc ggcacgtcac ggtcggcgcg ctggaggtcg
     6781 gcggtctctg ctgggacgcg gcctcggcca atctgcgcgg cgtggcccgc cggccgttgc
     6841 ccgagacggg cacgctgttc ttcgccgtcc ccgaggattg gagcctgctc gacgcggacg
     6901 gcctgaacct gcgccaggag gacggcatcg tgcggcttga gctcgaattc acgccgacgg
     6961 gcgccccgtg gcgcctggcg ttcgcccccg ccgccggcgc ggtcggcgcg tctcaggcgc
     7021 cggcgcagcg atcgctttcg gtttcgaact gaatcaggcc aggcggtcgc ccgccgtccg
     7081 catcagtcgt ggcgtcgcgc atgcggcgct ctcatcaaga aaggtcaatg ccatgattgt
     7141 caccaatgaa catcgccaga tgtaccgcga ggaaggctat ttcatcctcg aaaaggcgat
     7201 cgaccccgcc acgctgcgcc agttgcagga tgagtcggcc cgcctgaagc attgcttcga
     7261 cgagaagatg cgcgagtccg gcaccgacgt ctgggacatc agccactacg gcaaacgcta
     7321 cttcatctcc tcgtatcata cccagagccc gatcctggag cgctacctgt tcagcgaact
     7381 gatggcggag atctgccgcg ccacgctcgg gccggaggtc ttcctgttcc aggagcagtt
     7441 cgtgatcaag ggcgccgagg tcggcatgaa gttctcctgg catcaggacg gcggctggtt
     7501 ctatcccgac tgcccgccct acgtgacggt ctgggccgcg ctcgacgaca tgaccgagga
     7561 gaacggcacc atctatgtcc tgccctggtc gcacatggac cggcgcggct ggatcgagca
     7621 caaactctgc ccgcagacta acgaccgcgt cggctatgac gggcccgacc cgggcattcc
     7681 cgtctttgtc cccgccggca gcatcgtcgt cttttccaat cagacgctcc atcgcagcgg
     7741 gccgaacgtc tcgcccaacc tgcgccgcgc ctacctggcg caatattcct gcgccccgat
     7801 cctcaaccgg cacttcattt acgccgacta cgccgatccc gccgtgacgc ccgacagcct
     7861 cccgttcttc gcccgccgct tcctggagaa ctgggagatc aaccgccgcg acacctatcc
     7921 cctgcgccag ccgtatcatg tcgaggcgat gaaagcgccg gcgtaaagaa agcgcggccg
     7981 cggttcgcct gcacttgacc gatggaatgg ccaaacgaca aggaaccgcg ccagcggttc
     8041 gatatccaag ccgtgggttg aacgtccgcc cggcggccgt tcaacccatg ttcaccatcc
     8101 ccccaaatca aagaaccgag tgatcggttc gataactgat tttcgagacc cggagacgag
     8161 cgacgccaag ggacgaggtc cctggcctgg agcgacatgc tgaccggcgc ccgctggttc
     8221 ctacggcctg ggcaactggc agacctacga aacccttcag gacggcgtgc cggacacgca
     8281 agcccgcgag gtgacgacct tcaacaaata cgagacgcgg tcttgtcgga atttccgccc
     8341 ttttggcagg ggtcgggagc taaaccgcgt cgcgagttcg acggatcaga tcgaagcgtg
     8401 gatcattgat ttcatggatg gtgccggagg aggggctcga accctcatga ggttgccctc
     8461 actggatttt gagtccagcg cgtctgccaa ttccgccact ccggccaggg gggcgagcag
     8521 ccctatgcta acagagcccg gcgctcagtc aatcgccgcc tcgcgcctca tcgcctcgcc
     8581 ggacgaattt catggatcga tccagcccgc ggatcgcgct cggctcgccc gcccgcctgg
     8641 ggcctgcgcc ccgcagcccg cggcgccagg cggcggcaat cgaagagaat acgcggcgga
     8701 aatcagttgg cgggccggga ggagcgcggc gcgccggcgt cggggcgggc ggcggcgccg
     8761 gcggcgggcg gcgcgtcctg gcccaccggc agatcgatca ccacgcgttt gccgccccct
     8821 tcgcgcggct caagctgcag cttgccgtgc atccggctga gcaggctgcg cgggatcgtg
     8881 tagttgaaca gccgtccatg tttcatcgcc tggaacggca cgagcggatc ggcccattcc
     8941 ccggtgtcga attcatccag gtcgtcgccg ttgtcctcga tgcacagccg cagattccct
     9001 tcggcgaccg aaccgatgat ccagacccgg ctctcctcgg cgtcgggcgc gaacgagtgg
     9061 caggcgcggt gcaggatcgc gacgagcacc tgcagcagcg ccgtcgggtc gccgccgagc
     9121 gcgacgggtt cctggcccag attgacgcgg atgctgatcc ggtttttccc gatcggctcg
     9181 ctcagcagat cgagcgccgt ctgaatcacc agccccagat cgcactggcc ggcgtcggcg
     9241 cgcgacgggc gcgccacctt gaccagcttc tcgacgattt ccttgatctc ctgcgtcgtc
     9301 ttgtggatcg actccatgcg ttgcttctgc tgctcgccca ggttcttgtc catcatcagc
     9361 aactgcgtgt agccgaccag cgggaccatc ttgttgttga gctgatggcc caggcccgag
     9421 acgacggtgc ccatcgcggc cagtcgcccc gcgccgtgga gcgactggtg aaactgcttc
     9481 agccgggcga cggcgtgctc cagctcggcg ttgcgcgcgc gcacctcgct cagcaggtgg
     9541 gccttctcga tggcgaggcc gcagatattg gtcagcgtgc gcagcagcgg ccggacggcg
     9601 cgcagcgcat gcgccgtctc ctcgctcccc tccaccgcca gcaggccgaa acagcgctcg
     9661 ccgctggcga tcggcgccgc cagcatcgag gcaaagccga tcttgcggaa cagccattcc
     9721 tcgggggcgg gaaagccgct gaactcgtgc caactgacgt cctggctctt caggatcgcg
     9781 tcctccagct tcgcatgccc cagatgcact tctcggatct gctcggcggg acacgccagc
     9841 ggcgcccctt cgtggtggat caggcgcatc acgcgcccgt cctccccgag caggaagacg
     9901 atcccgcgcg ccgacttgag cgcctgcatc accgtccgca ggatcatccg gccaatctcc
     9961 acgggatcgt tttcgcgaaa cgcctgttcg gtgaactgat tgatgtgcga gagcagcagg
    10021 acttcttcca ccatcagcga gtgcaggctc tccaaggtcg gctcggggac caactcgcgc
    10081 ggcggctcgt ccaccagcag caggacgtcc agccgggtct gctgcagcat ccagacgacc
    10141 tgctcgctgc aattgaccag ccgaaccgag ccgttgagcc gccgcatcgt gttatggaat
    10201 tcaaccagca gccccagcgc gctcgaattg atgaacggcg tctcctccag atcgaaaatc
    10261 agcgagcgat agccgttatc gaccagcagc cggatctgct ggcggtaatg ttccacctgc
    10321 agcgcatcca gattgccgta ggtccggatc accagcgtac gggaatgagg gtgacgtgtg
    10381 atcatgttca agcgtcgcaa cccgccgtct ctcgcggctg atggatgggc aagagctgcc
    10441 ccccttcgga caaaagcacg cgaacgccgg ggaatggtcc cgccgccggc ggattgctct
    10501 ccgctcatga cttatcgacc agccgcgccg gtcgcttgag gaatgattat tttattgaag
    10561 ttacacgcgg tcgcgtcagt actgacgcgt gatcacataa tcgggcagga ttttcatata
    10621 ttcggcggcg acttcgtcat acaccgtcag gccgtccaga tgcgcgaccg cctgccgcgc
    10681 gaaccgccgg gcggtctcca gggcctcatc gatgcagccg taacgttcca ggatcgcgag
    10741 catctcgccg ttggcggcgg cgaccgaccc gccgttctcc aggatcgatt cgatcgtcct
    10801 gcgctcggca tcggtcgcct ggcgcagcgc caggatcagc ggcagcgtct gcttgcccgt
    10861 cgccatgtcg atccccaccg gcttgcccca gagggcgtcg gtggcggtca ggtccagcgc
    10921 gtcgtcggtg atctggaacg ccagcccgaa ctccagcccg aaggccgaca gccgggcgat
    10981 cgtcacggga tcgagcccgc cggcgatccc gccgacggcc gcgcaggccg aaaacaggca
    11041 ggccgtcttg caggtgatca ggtggatgta atcctcgggc tcgagccacc ggccccgctt
    11101 ttcgatctgg aacatctcgc cctcgcacat ccggcgggtc acctggcaga tcagccgcag
    11161 cggctcatgg tccaggtagt tcagcgagag ttcgaacgcg gcggcgtaga ggtaatcggc
    11221 catcaggatg gcgacgtcgt cgccccagcg ggcgttgacc gacgggcgtc cgcgacgcat
    11281 cggcgccttg tcgatcacgt cgtcgtgcat cagcgtggcc acatgcaccg cctccatcgc
    11341 ggcggccagt tgcaccggcg cggggctggg tgcattatag gcccgtgcgg ccagcagcgt
    11401 gatcatcggc cggagcttct tgccccccgc cagacagata tactcgccga cctcgcggac
    11461 caggctggat tgcgaatcca gaatgtcgtg caggacgcgg tcgacggcgg ccaactcgcg
    11521 ctccaggtat tgagcgatct cgacgaccag attgtcgggc tgggcgggtt gtttgggctg
    11581 agcgggcatc gaaacgtcgc gcctcggggt ggcgttggtc gaactgagcc atctgcgtgc
    11641 aaggatcggg gccgagcgcc ctgacgacag gttaatcgcc cccccgcttg aacgcaagcc
    11701 gtttccagtc aaatggctgg ctcctgaaat cgcgggagga cgcgcgcctt gcgcgatccc
    11761 gcg
//
LOCUS       DAOPCO010000109        11748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5388_length_11748_cov_6.065937, whole genome shotgun sequence.
ACCESSION   DAOPCO010000109 DAOPCO010000000
VERSION     DAOPCO010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11748
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5388_length_11748_cov_6.065937"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..615
                     /locus_tag="PLS90_09100"
     CDS             85..615
                     /locus_tag="PLS90_09100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPK02600.1"
                     /translation="MPETLAAAPWQGANLSFWLIAALVLISGGFAAFTRNIVQAAYSL
                     FFTLVGMAGLYILLGSPFLAITQVIIYVGGILVLLMFGVLLTNRPLERARRGQGRLYV
                     VGAVVAALALAMVLLRIVFTTEWMTGSAAVEPVGGTRPIGRLLLTEYLLPFELAGMLL
                     LLGLIGAAYLVRRRET"
     gene            612..965
                     /gene="nuoK"
                     /locus_tag="PLS90_09105"
     CDS             612..965
                     /gene="nuoK"
                     /locus_tag="PLS90_09105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPK02601.1"
                     /translation="MSGLSAFLALDGGAPVGLHAYLLVAALLFAFGLTACLARRNAIG
                     ILIGIELILNAAILNFMAFWRFGASGDDVSGPMFGIFMIILAACEAAVALAVLLNLYF
                     NFGTVEVDDIAQLRR"
     gene            1012..3129
                     /gene="nuoL"
                     /locus_tag="PLS90_09110"
     CDS             1012..3129
                     /gene="nuoL"
                     /locus_tag="PLS90_09110"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPK02602.1"
                     /translation="MSDGVTLFLALGIFLSPLAGFILLAFLNRRLPRNGDWLATALIA
                     LPLLLAVALFARALRSPSPLLLNLTFTWLPMTTAAGLDGGLMVDRLTAVMLLVVTLVS
                     FLVHLFSTGYMRGDARYGRYFTCLQLFTLSMLGLVLANNLLFLYIFWELVGLSSYLLI
                     GHWFEKKSASNAAIKAFITTRLGDVGMFIGIMICYLEVGSLQYADLFAAVETGTLAGA
                     WRTIAGLGIFIGAMGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYLTGRLLP
                     LFDPQTLLGIAYIGAFTALFAASIALVQDDIKKVLAYSTVSQLGYMILALGVGGYVSG
                     LFHLTTHALFKAGLFLGSGAVIHAMHHEQSMSRYGGLARKMPWTAVCYLLATLALVGF
                     PMFSGFWSKDAILSDALAFSILSEGHWFLPAAGFATVFMTAFYMFRQYFLTFTGAPRD
                     RHAYDHAHEVGWSMRAPLVILGILAVVGGGLTGKWFGAMNPSRSGVAQVAAFHEAGHR
                     SPWVAAALAAVPDSHAAEHAADGAAAMHGDDHAAHVHHQAHRRAMILSTGLGLLGILL
                     GMLMYYERRGGATVLQPAVFAARLRPLHTLLLNKYFIDELYHWLFVRTTVVAGWFSAL
                     FDRRAIDAVVNLVGRLGWLGGWLIEIVDRVAVDWFLVMGSARAVSFAGDRMSYAHTGR
                     VRQYMVYSAVGLVVLGGFVVWWM"
     gene            3190..4707
                     /locus_tag="PLS90_09115"
     CDS             3190..4707
                     /locus_tag="PLS90_09115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPK02603.1"
                     /translation="MLLSLITFLPLVGMVIVLALPGERHGLIRWTAVGFTGVVFALTL
                     YLWHIFDPGSIQAGTAYSGYDARTLIEIPWIKPYHIYYRFGVDGLSVLLIVMTGLLSF
                     LATFVAFPIEKQVKGFFALYLLLVTGMFGVFMALDFFLFYVFWEVMLLPMYFLIGIWG
                     GPRKEYAAIKFFIYTLLGSVLILLVMLAYYFKMKSIGLEALAFNIPELIARRPFSVPG
                     GPTLFQHLLFWGLFIGFAIKVPIFPFHTWLPDAHVEAPTAISVILAGVLLKMGGYGIL
                     RLNYPLLPEVGAHEAVMFFLAVLGVVNIIYGAFCALGQKDFKRLVAYSSVSHMGFVLL
                     GIAVLKEAAVNGAIFQMFAHGITSAMMFTLVGVLYERAHHRDIERFGGIALQMPVYFA
                     LAVVGFFASLGLPSMVGFIGEVMVFLGAFTYSPWIAGIATIGMLLTAGYILWTMQRVY
                     LGPKREEHAGFADVNRWEAISLVPLAAVAILLGVLPSIAINIYHTSTAVFMTLFR"
     gene            4716..6212
                     /locus_tag="PLS90_09120"
     CDS             4716..6212
                     /locus_tag="PLS90_09120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPK02604.1"
                     /translation="MTVNELLHNLMATLPELILGVGICAVILADMLTPAARGRQVTAA
                     LALLAVVWGLTLTAAMPAGTGGDFGGMLARDGLARVFKLLFLTGAASTILFSLRSRET
                     IGYRQGEFHALVLGATLGACLLAAADNIILLVLALETLSISSYVLAGYVKHERRSAEA
                     ALKYLLYGAVASGIMLFGFGYLYGMTGTTSISTAAAALAAGPPNRLAFTLALLLVLVG
                     FGFKMAMVPFHFWCPDVYQGAPTPVTAYLAVVSKAAGFAALMRVLLPVFEAPAAFATL
                     TGSGQLALLFGILAAVTMLVGNFIALRQTDIKRLLAYSSIAHAGYLLMGFTVFSPDAA
                     ETILFYFVIYLFMNLGAFWCAIVLIEQTGSAELDSFRGAAWRSPFLGGVMFIFLISLT
                     GLPPTAGFVAKLRLFEVVLSAGMAGLEGGAAAAGYAYLALAIFGVLNSAVSLYYYMKI
                     MRAMALERPASREPIDLSAADRLYALLFAVPTVALLFFTPVLRLVDIL"
     gene            6239..6730
                     /locus_tag="PLS90_09125"
     CDS             6239..6730
                     /locus_tag="PLS90_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02605.1"
                     /translation="MTAPELKAHELAALNRAFNDRFMSLARYALDAQPYVPEKMAWAL
                     EDLRRIAEADRRAADRLAEWIAEHGGIAQTQPIPPRIAEWNYLALDFILNSLIDELTA
                     QRERYRRELADIQTPAVRDMLGWLIRQLDDDIIRLRSHSGEPAAARGDKAQAPGDEAR
                     AEN"
     gene            complement(6727..10872)
                     /locus_tag="PLS90_09130"
     CDS             complement(6727..10872)
                     /locus_tag="PLS90_09130"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPK02606.1"
                     /translation="MPFPSARRRPAMGLVLLFAGWLWPAPAANARVWHVDDDAADQPA
                     ADFATIQAGIDAAAEGDTLVVHPGRYFEVLRLADKNLHIRGADPTSPAIVEATQIDAQ
                     LVDSVVVFSGRVGPATILEGLTLLNGSAQDGGGLRSAGDRPTSPTIRNNIIRLNFAID
                     RGGGIFRCDGLIERNRIESNEADRGGAIQGCAGVVRANTLSDNVARLGGAAAECPAAF
                     ESNVLRRNEARLQAGALYRCSGPIRDNAFVANSSAEDGGALHSCHGLIENNRFLHNLA
                     RQGGAIFGCNGTIRGNHVEKNRGNLGAGVMQCHGLIEHNLLVANVALQMGGGLAVCGG
                     TIRLNLIEGNVALAAGGGLAQTTAVVEFNQISDNHAPRGAGLARCSGKVRRNRIERNY
                     AEQAGGAIEESFLELDSNLIRGNGAPRGGALYACGGTVQNNTFVANWCDDGQAVIDIA
                     TRPMMFRNNIVAFANGAALRIPAGEPIALVSRHNLFHEIAGLLLLTPDGAAHLQPEQL
                     DQLHDWSDGNRTGDPRFVDAGGKDGDPALGDDDDFRLTLDSPAIDAGLPDVLLNLPAL
                     DLSGAARLAGDAVDLGAYEWGAAPDSDGDGLDDDRELALGADPTRIDSDGDGADDSFE
                     WLRGTPPGVAGTPVGLEVAPGDGVQLAIAMAYEGERIRIAPGVYYETLDSQGKNLHLT
                     GWDPGSAQTRDATVIDATGRGPVLKLAGTEDRRFILAGLTLRRGYAVQHSGIDGRGAS
                     PVLRNLAVRDCRTTGGATVAQCTGPIVDCEFSGNQAVIGAALWQCHGEIRGNQFIGNR
                     AVDGGALANCNGPIEDNLFDANHAIDEGGALYVCNGPVVRGNRFIRNQALFGGAIYRG
                     SSRVIENEFIANIGRRGGGAIQFVSGVISDNYCEDNFANFGAAFSTCNGEIRRNTFTN
                     NRAHVQGGALYSCSGEIKQNQMDGNSAYQGGALYSCSGEIAFNSLRRNEAVREGGALY
                     GCSGRIRGNRIEDNLAQYGGAIWFSSAIFENNDVIGNVATRQAGGIGDSTGVFRRNVV
                     MNNSSEEAGAFLRFDGDIQHNVIRGNSAVREGGALRDCRGRVMNNLIEGNRAWRGGAM
                     QGCSADVINNTFIGNQAIPGPAGEPVQGGALHNASGRIINNIFQANGHIALYSGDPDP
                     PRPGEQTPAPVVPRELRRNLFFAHYEAIYGTLAGLRVTSASSLDDVLPHSRENWEGDP
                     LLEDADGPDGDPATFADNRPWLAPGSPAIDAGDDGVNPAGADLAGGPRYHAALAAESV
                     IDLGAYEFFGVVISAHGSFADLRPGSSFTVEWLCSPAAGSAVRLELLRFGAVIAELGT
                     DWSPLGRHAVTLRIPKDLPAGEGYQLRVSSLWNPVYEDTVPRDGFALKAERNAAADWR
                     QYY"
     gene            complement(11043..>11748)
                     /gene="lgt"
                     /locus_tag="PLS90_09135"
     CDS             complement(11043..>11748)
                     /gene="lgt"
                     /locus_tag="PLS90_09135"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007360342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPK02607.1"
                     /translation="LRHYHRRGRSPLDPDRIYTAITALALGVLIGGRLGYLLLYAPGD
                     TLRDPLLVFRIWEGGMASHGGFLGVAVALAWLARRFKVSFLLLGDLLCSIAPLGLLLG
                     RIANFINGELPGRVTDVPWAVIFPTSAPPGTPLSQIPPRHPSQLYEAGLEGLLLFVYL
                     QWRFWRTNALATPGRLSAEFLLLYAIVRMVGEQFREPDAPPILGLTRGAFYSLFLILA
                     AGAVWAWSRRSNRAQP"
BASE COUNT         1740 a         3633 c         4084 g         2291 t
ORIGIN      
        1 gaaaaaggaa gccgagacca aggccgcggc cgaggcctcc gctccccagg ccggcgacgc
       61 cccgacgcgg aatggagcag cctgatgccc gagacgctcg ccgccgcccc ttggcaaggc
      121 gccaacctca gtttctggct gattgccgcg ctggtgctga tttccggtgg gttcgcggcg
      181 tttacgcgca acatcgtgca ggccgcctat tcgctgttct tcacgctggt cggcatggcc
      241 gggctctata ttctattggg ttcgccgttt ctggccatta cgcaggtcat tatttatgtc
      301 ggcggcattc tggtgctgct gatgttcggc gtgctgctga ccaaccggcc gctcgagcgc
      361 gcccggcgcg gccagggtcg gctctacgtc gtcggcgcgg tcgtcgcggc gctggcgctg
      421 gccatggtgc tgctgcggat cgtcttcacg accgagtgga tgaccgggtc ggccgcggtg
      481 gaaccggtgg gggggacgcg gccgatcggc cggctgctgc tgaccgaata tctattgccc
      541 tttgagctgg ccggcatgct gctgctgctc gggctgatcg gcgcggcgta tctggtgcgg
      601 aggcgggaga catgagcggc ctgtcggcat ttctggcgtt ggacggcggg gcgccggtgg
      661 ggctgcatgc ctacctgctg gtggccgcgc tgctctttgc atttggttta acggcctgcc
      721 tggcgcggcg caatgcgatc gggattctga ttggcattga gctgatatta aacgcagcga
      781 ttctgaattt catggcgttt tggcgcttcg gcgcttcggg cgacgatgtg agcgggccga
      841 tgttcggcat tttcatgatc attctcgccg cgtgcgaggc cgcggtcgcg ctggcggtgc
      901 tgctcaatct gtatttcaac tttggcacgg tcgaggtcga cgatatcgcc cagttgaggc
      961 gctgattccg ccggaagcag tcaggccgac acgaggaaac cacggacaga catgagcgac
     1021 ggcgttacac tatttctggc attgggcatt tttctttcgc cgctggcggg gttcatcctg
     1081 cttgccttcc tgaaccggcg tctgccgcgc aacggcgact ggctcgccac ggcgctgatc
     1141 gcgctcccgt tgctgcttgc cgtcgccttg ttcgcgcggg cgttgcgttc accgtcgccg
     1201 ctgctgttga acctgacatt cacatggctg ccgatgacga cggcggcggg gttggacggt
     1261 ggtctgatgg tcgaccggct gaccgccgtg atgctgctcg tcgtcacgct ggtcagcttt
     1321 ctggtgcacc tgttttcgac cggctacatg cggggcgacg cgcgctacgg gcgctatttc
     1381 acctgcctgc agcttttcac gctgtcgatg ctcgggctcg tcctcgccaa taacctgctt
     1441 ttcctttaca ttttctggga actggtcggg ctttcctcgt atctgctgat cggccactgg
     1501 ttcgagaaga aatcggcgag caatgcggcg atcaaggcgt tcattacgac gcggctgggc
     1561 gacgtcggca tgttcattgg cattatgatt tgttacctgg aggtcggctc gctgcagtat
     1621 gccgacctgt tcgcggcggt cgaaacgggc acgctcgccg gcgcctggcg gacgatcgcc
     1681 gggctcggca ttttcatcgg cgcgatgggc aagagcgcgc agtttccgct gcacgtctgg
     1741 ctgcccgacg cgatggaagg cccgacgcct gtcagcgccc tgatccacgc cgccacgatg
     1801 gtcgccgccg gagtgtatct gaccggccgg ctgctgccgc tgtttgaccc gcaaacgctc
     1861 ctgggcattg cctacattgg ggcgtttacg gcgctcttcg cggcgagcat tgcactggtg
     1921 caggacgaca tcaagaaggt gctggcctac tccaccgtca gccagctcgg ctacatgatc
     1981 ctcgcgctcg gcgtcggcgg gtatgtttcc ggtctgtttc acctgacgac gcacgcgctg
     2041 tttaaggccg gtctgttcct cgggtccggc gccgtgatcc acgcgatgca tcacgagcaa
     2101 tcgatgagcc gctacggcgg cctcgcgcgc aagatgccgt ggacggcggt ctgttatctg
     2161 ctcgcgacgc ttgcgctcgt gggttttccg atgttttccg gtttctggag caaggacgcc
     2221 atcctcagcg atgcattggc attttcgatc ctgagcgagg gacactggtt cctgcccgcc
     2281 gccgggttcg ccaccgtctt catgaccgcg ttctacatgt tccggcagta tttcctgacg
     2341 ttcaccggcg cgccgcgcga ccgtcacgcc tacgatcacg cgcatgaagt gggctggtcg
     2401 atgcgcgcgc cgctggtcat cctggggatt ctggcggtgg tgggcggcgg actcaccggc
     2461 aagtggttcg gcgcgatgaa cccgagccgg agcggcgtgg cgcaggtggc ggcgttccat
     2521 gaggcgggcc accgttcgcc gtgggtcgca gcggcgctgg cggccgtgcc ggattctcac
     2581 gcggcggagc atgcggcgga cggcgccgcc gcgatgcacg gcgacgatca cgccgcccac
     2641 gttcaccacc aggcgcaccg gagggcgatg attctttcga ctggtttggg gctgctgggg
     2701 attctcctgg gcatgctgat gtattacgag cggcggggcg gcgcgaccgt gctgcagccg
     2761 gccgtattcg ccgcgcgtct ccggccgctc cacacgctgc tgttgaacaa atattttatt
     2821 gatgagctat accattggct tttcgtgcgt acgacggtcg tggccggctg gttcagcgcg
     2881 ttgttcgacc gccgcgcgat cgatgccgtg gtcaacctcg tcggccggct gggctggctt
     2941 ggcggctggt tgatcgaaat cgtcgatcgc gtcgccgtgg actggttcct ggtgatgggc
     3001 tcggcgcgcg ccgtcagctt cgcgggcgac cggatgtcgt atgcgcacac cggacgcgtg
     3061 cggcaataca tggtttattc ggccgtgggc ctggttgtgc tcgggggatt tgttgtctgg
     3121 tggatgtgat gggacgggct ttgaggatga ataaaaggac gaggacgagg gactgacgat
     3181 atggatcaga tgctgctcag tttgatcacc ttcctgccgc tggtcggcat ggtcattgtg
     3241 ctggcgctgc cgggtgaacg ccacggcctg atccgctgga ccgcggtcgg cttcacgggg
     3301 gtagtcttcg cgctgacgct ttatctgtgg cacattttcg atcccggctc gatccaggcg
     3361 ggcacggcct attcgggcta cgacgcccgc acgctgatcg agatcccgtg gatcaagccg
     3421 tatcacatct attaccggtt cggcgttgac ggcctctccg tcctgctgat tgtcatgacc
     3481 ggcctgttgt ccttcctcgc cacgttcgtc gcttttccga tcgagaaaca ggtcaagggc
     3541 tttttcgcgc tttatctgct gctggtcacc ggcatgttcg gagtcttcat ggcgctcgat
     3601 ttcttcctgt tttacgtttt ctgggaagtc atgctgctgc cgatgtattt tctcattgga
     3661 atatggggcg ggccgcgcaa ggaatacgcg gccatcaagt tctttatcta cacactgctt
     3721 ggctcggtgc tgatcctgct ggtcatgctg gcgtactatt tcaagatgaa gtcgatcggg
     3781 ttggaggcgc tggcgttcaa catcccggaa ttgatcgcgc ggcgtccatt cagcgtgccc
     3841 ggtggcccca cgctcttcca gcatctgctg ttctggggcc tgttcattgg atttgcgatc
     3901 aaggttccga ttttcccgtt ccacacatgg ctgcccgatg cccacgtcga ggcgcccacc
     3961 gcgatttccg tcattctggc gggcgtgctg ctcaagatgg gcggctacgg cattctgcgg
     4021 ctgaactacc cgctgctgcc cgaggtcggc gcgcacgagg ccgtgatgtt cttcctcgcg
     4081 gtgctggggg tcgtgaatat catctacggc gcgttctgcg cgctgggaca gaaggatttc
     4141 aagcggctcg tcgcctattc ctcggtcagc cacatgggat tcgttctgct ggggatcgcc
     4201 gtgctgaagg aggcggcggt caacggcgcg atcttccaga tgttcgcgca cgggatcacg
     4261 tcggcgatga tgttcacgct ggtgggtgtc ctttacgagc gcgcgcatca ccgcgacatc
     4321 gaacgcttcg gcggaatcgc gctgcagatg ccggtttatt tcgcgctcgc ggtcgtcggg
     4381 ttcttcgcct cgctcggtct gccgtcaatg gtcggcttca tcggcgaggt gatggtgttc
     4441 ctgggcgcgt tcacctacag cccatggatc gccggcatcg cgacgatcgg catgctgctg
     4501 accgccggct acattctgtg gacgatgcag cgcgtttatc tcggcccgaa aagagaagag
     4561 cacgccggct tcgcggatgt caaccgctgg gaggcgattt cgctcgttcc cctggccgcg
     4621 gtcgcgatcc tgcttggggt gctcccctcg attgccatta acatttatca cacttcgacg
     4681 gcggtcttca tgacgctgtt ccgataggcg cggcgatgac ggtcaatgaa ctgttgcaca
     4741 atctaatggc cacgctgccc gagctgatcc tcggcgttgg catctgcgcg gtcattctgg
     4801 ccgacatgct gacgccggcg gcgcgcggtc ggcaggtgac ggcggcgctg gccctgctgg
     4861 cggtggtgtg gggcttgacg ctgacggcgg cgatgccggc gggcacgggg ggcgatttcg
     4921 gcggcatgct ggcgcgggac gggctggcgc gcgtcttcaa gttgttgttc cttaccgggg
     4981 cggcgtcgac gatcctgttc tcgctgcgca gccgcgaaac gattggctac cggcaggggg
     5041 aattccatgc gctggtgctc ggcgcaacgc tcggggcctg cctgctcgct gccgccgata
     5101 acattattct gctggttctc gcgctggaaa cgctgtcgat ctcgtcctat gtgctggccg
     5161 gatacgtcaa gcacgaacgg cggtcggccg aagccgcgct caaatatctc ctctacgggg
     5221 ccgtcgcttc cggcatcatg ctgttcggat tcggttatct ttacggcatg accgggacga
     5281 cctcgatttc cacagcggcc gccgcgctgg cggccgggcc gccgaacagg ctggccttca
     5341 cgctggccct gctgctcgtg ctggtcggct tcggcttcaa aatggcgatg gttccgtttc
     5401 atttctggtg ccccgacgtt tatcagggcg cgccgacgcc ggtgaccgcg tatctggcag
     5461 tcgtttccaa ggcggcgggc ttcgcggcgc tgatgcgcgt gctgctgccg gtgttcgaag
     5521 cgcccgcggc attcgcgacg ctgaccggct ccgggcagct ggcgctgctg tttggaattc
     5581 tggctgcggt tacgatgctc gtcggtaatt tcattgccct gcgtcagacc gacatcaagc
     5641 gcctgctggc ctattcgtcg attgcccatg cgggctatct gctgatgggc tttacggttt
     5701 tttcgccgga tgcggcggag acaattctgt tctacttcgt gatctacctg ttcatgaacc
     5761 tgggcgcgtt ctggtgcgcg atcgtactga tcgaacagac ggggagcgcg gagctcgatt
     5821 ccttccgcgg cgcggcgtgg cgctcgccgt ttctgggcgg cgtgatgttt attttcctga
     5881 tttcgctgac gggcctgccg ccgacggcgg ggttcgtggc gaagctgcgg ctgttcgaag
     5941 tcgtgctgag cgccggcatg gccgggctgg aggggggcgc cgcggcggcc ggctatgctt
     6001 atctcgcgct ggcgattttc ggcgtcctca acagcgcggt ctcgctttat tattacatga
     6061 aaatcatgcg cgcgatggcg ctcgagcggc ccgcgagccg ggagccgatc gatctgagcg
     6121 ccgccgaccg gctctacgcg ctgctgttcg ccgtcccgac ggtcgcgctg ctgtttttca
     6181 cgccggtcct gcggctggtt gatattctgt aaggcgggcg agaggaatgg atcgagccat
     6241 gacggcgccg gagctcaagg cccatgaact ggcggcactg aatcgcgcgt tcaacgaccg
     6301 gttcatgtcg ctcgcgcgct atgcgctcga tgcccagccc tacgtgccgg aaaagatggc
     6361 gtgggcgctg gaggatctgc ggcggatcgc cgaggcggac cgccgcgccg ccgaccgcct
     6421 ggcggaatgg atcgcggaac acggcggcat cgcacagacg cagccgattc cgccgcggat
     6481 tgccgaatgg aactatctcg cattggattt catcttgaac agcctgatcg atgaattgac
     6541 cgcccagcgc gagcggtatc ggcgcgagct ggcggatatc cagacgccgg ctgtgcggga
     6601 tatgctgggc tggttgatcc gccagcttga tgacgacatc atccgcctgc gcagccacag
     6661 cggcgagccg gccgccgcgc gcggggacaa ggcgcaagcg ccgggggacg aagcgcgcgc
     6721 cgaaaattaa taatattgcc tccagtcggc ggccgcgttg cgctcggcct tgagcgcgaa
     6781 tccatcgcgc ggcacggtgt cctcatacac cggattccac aggcttgaca cgcgaagctg
     6841 gtagccttcg ccggcgggca gatccttggg gatccggagc gtgacggcat ggcgtccgag
     6901 cggactccag tccgttccga gttcggcgat gacggcgccg aagcgcagca gttcgaggcg
     6961 cacggcgctg ccggccgcgg gactgcagag ccattcgacg gtgaagctgg acccggggcg
     7021 gagatccgcg aacgagccgt gggccgagat cacgacgccg aagaattcat aggcgccaag
     7081 gtcgatcacg ctctccgcgg ccagcgcggc gtgatagcgg ggtccgccgg cgagatcggc
     7141 gccggccgga ttgacgccgt cgtcgccggc gtcgatcgcg ggcgaccccg gcgcgagcca
     7201 cggccggttg tcggcgaacg tggcgggatc gccgtcgggt ccgtcggcgt cctcgagcag
     7261 cgggtcgccc tcccagttct cccggctgtg ggggagcacg tcgtcgaggc tggaggcgga
     7321 ggtcacgcgg agtccggcga gcgtgccata gatcgcttcg tagtgggcga agaagagatt
     7381 gcggcgcagc tcgcgcggca caacgggagc aggcgtctgc tcgccggggc gcgggggatc
     7441 gggatcgccg ctgtagagcg cgatatggcc gttcgcctga aaaatattgt taatgatccg
     7501 gccggaggcg ttgtgcaggg cgcctccctg caccggctcg cccgccgggc cggggatcgc
     7561 ctgattgcca atgaaggtat tattaatgac atcggcggag cagccctgca tggcgccgcc
     7621 gcgccaggcc cggttcccct cgatcagatt gttcattacc cggccgcggc agtcgcgcag
     7681 ggcgcccccc tcgcgcacgg ctgaattgcc gcgaatcaca ttgtgctgaa tatcgccgtc
     7741 gaagcggagg aacgcgccag cttcttccga gctgttgttc atgacgacgt tacggcggaa
     7801 cacgccggtc gagtcgccga tgccgccggc ctggcgggtc gccacattgc ctatcacgtc
     7861 attgttttcg aagatggcgg aggagaacca gatcgcgccg ccgtattgcg ccaggttgtc
     7921 ctcgatgcga ttgccgcgga tccttcccga gcagccatag agggcgcccc cttcgcggac
     7981 ggcctcattg cggcgcaggc tgttgaaggc gatctcgccg gaacaggaat agagtgcacc
     8041 gccctgatac gcggaattgc cgtccatctg gttctgcttg atctcgccgg aacaggaata
     8101 gagcgcacca ccctgcacgt gggcgcggtt gttggtgaag gtgttgcggc ggatttcgcc
     8161 gttgcaggtg gagaacgccg cgccgaaatt ggcgaaatta tcctcgcaat aattatcgct
     8221 gatcacgccg gagacgaact ggatcgcgcc gccgccgcgg cgcccgatat tggcaatgaa
     8281 ttcattttca atgacgcggc tggagccgcg gtaaatcgcg ccgccgaaaa gagcctggtt
     8341 gcggatgaac cggtttccgc gaacgaccgg gccgttgcag acatagagcg cgcccccttc
     8401 gtcgatcgcg tgattggcgt cgaacaggtt gtcctcgatc gggccgttgc agttggccag
     8461 ggcgccccca tcgacggcgc gattgccgat gaattgattg ccgcgaattt cgccgtggca
     8521 ctgccacagt gcggcgccga tcacggcctg attgccgctg aattcgcagt cgacgatcgg
     8581 cccggtgcac tgggcgacgg tggcgcctcc cgtggttcgg cagtcgcgga cggcgaggtt
     8641 gcgcagcacg ggcgaggcgc cccgcccgtc gatgccggaa tgctgaacgg cgtagccccg
     8701 gcgcagcgtc aggcccgcga ggatgaagcg ccggtcctcc gtgcccgcca gcttgagcac
     8761 ggggccgcgc ccggtcgcgt cgatcacggt tgcatcgcgc gtttgcgcgc tgcccgggtc
     8821 ccagccggtc agatgcaggt tcttgccctg gctgtcgagc gtttcgtagt agacgccggg
     8881 cgcgatgcga atccgttcgc cttcgtaggc catcgcaatc gcgagctgga cgccgtcgcc
     8941 gggcgcgact tccaggccaa cgggcgtccc cgcaacgccg gggggcgtgc cgcgcagcca
     9001 ttcgaagctg tcgtcggcgc cgtcgccgtc cgaatcgatc cgcgtggggt cggcgcccag
     9061 cgccagttcg cggtcgtcgt ccaggccgtc gccgtcgctg tcgggcgccg cgccccattc
     9121 gtaggcgccc aggtccacgg cgtcgccggc cagccgggcc gcgccggaca ggtcgagcgc
     9181 gggcaggttc agcagcacgt cgggcaggcc ggcatcgatg gccggcgaat cgagggtcag
     9241 ccggaaatca tcgtcgtccc cgagggcggg atcgccgtcc ttgccgccgg cgtcgacgaa
     9301 tcgcgggtcg cccgtccggt tcccgtcgct ccagtcgtgg agttgatcaa gctgctccgg
     9361 ctggaggtgc gccgcgccgt cgggcgtcag cagcagcagc cccgcgatct catggaacag
     9421 gttatggcgg ctgaccagcg cgatcggctc gccggcggga attcgcaggg cggctccatt
     9481 ggcgaacgcc acgatgttgt tgcggaacat catcggccgg gtggcgatat cgatgacggc
     9541 ctgaccgtcg tcgcaccagt tggcgacgaa ggtgttgttc tgcaccgtgc cgccgcaggc
     9601 gtagagcgcg ccgccgcggg gggcgccgtt gccgcggatc agattgctgt cgagttcaag
     9661 gaaggactcc tcgatcgcgc cgccggcctg ctcggcgtag ttgcgctcga tgcgattgcg
     9721 gcggaccttg cccgagcagc gagcgaggcc ggcgccgcgc ggcgcgtgat tgtcgctgat
     9781 ctgattgaac tcgaccacgg cggtcgtctg ggccaggccg ccccccgccg ccagggcgac
     9841 gttgccctcg atcagattga gccggatcgt gccgccgcac accgccaggc cgccgcccat
     9901 ctggagcgcc acgttggcga cgaggagatt gtgctcgatc aggccgtggc actgcatcac
     9961 gccggcgccc aggttgccgc ggttcttctc gacgtggttg ccgcggatcg tgccgttgca
    10021 gccgaaaatc gctcccccct ggcgcgcgag attgtggaga aagcgattgt tttcgatgag
    10081 tccgtggcag gaatgcagcg ccccgccgtc ctcggcgctg gaatttgcaa cgaaggcgtt
    10141 gtcgcggatc gggccggagc agcgataaag cgcgccggcc tggaggcgcg cctcgttgcg
    10201 gcgcaagacg ttggactcga aggcggcggg gcactcggcg gcggcgccgc ccagccgggc
    10261 gacgttgtcc gacagggtat tggcgcggac gacgccggcg cagccctgaa tcgcgccgcc
    10321 gcggtcggcc tcgttggatt cgatccggtt gcgttcgatg aggccgtcgc agcggaagat
    10381 gccgccgccg cggtcgatcg cgaaattgag ccggatgatg ttattgcgga tcgtcggcga
    10441 ggtgggccgg tccccggcgc tgcgcagccc gccgccatcc tgcgccgagc cgttcagcag
    10501 ggtcagcccc tcgaggattg tcgccgggcc gacgcggccg gagaacacga cgaccgaatc
    10561 gaccagttgg gcgtcgatct gcgtcgcttc gacgattgcc ggcgaagtag gatcggcgcc
    10621 gcggatatgc aggttcttgt cggcgaggcg caggacctcg aagtagcggc cgggatgaac
    10681 gacgagcgta tcgccctcgg cggcggcgtc gatgccggcc tgaatggtgg caaagtcggc
    10741 cgccggctga tcggcggcgt cgtcgtccac gtgccagaca cgggcgttgg ccgcgggggc
    10801 gggccacagc cagccggcaa agagcagaac cagccccatc gccggccggc gtctggcgga
    10861 gggaaatggc atgaatcgca tcagtccgtg gagagggaaa gggaacgatc cgatcggggc
    10921 ggcgcttcgc cgccgctgga tcagtatagg ccctcccgcg ccgccggggc aacccggctg
    10981 gggaaattaa ggacggatcg ggccgatccg gcgcgttctg gaaacggggt aagctggatg
    11041 ggtcagggtt gtgcgcgatt ggatcggcgc gaccaggccc agacggcgcc ggcggccaga
    11101 atcagaaaca ggctgtagaa cgcgccgcgc gtcaggccga ggatcggcgg cgcgtcgggt
    11161 tcgcgaaact gttcgcccac catgcgcacg atggcgtaga gcagaaggaa ctccgcggac
    11221 agccggccgg gcgtggccag cgcgttggtg cgccagaacc gccattgcag gtagacgaac
    11281 aggagcaggc cctcgaggcc cgcctcgtag agctgcgaag gatggcgcgg cgggatctgg
    11341 ctgagcggcg tgcccggcgg ggcgctcgtg ggaaagatca cggcccacgg cacgtccgtg
    11401 acgcggccgg ggagctcgcc gttgatgaaa ttcgcgatcc ggcccagcag caggccgagc
    11461 ggggcgatcg agcagagcag gtcgcccagc aacagaaacg acaccttgaa gcgtcgcgcg
    11521 agccaggcca gcgcgacggc cacgcccagg aagccgccgt ggctggccat ccccccctcc
    11581 cagatccgga agacgagcag gggatcgcgc agcgtgtcgc cgggcgcgta gagcagcagg
    11641 tagccaagcc ggccgccgat gaggacgccg agggccagcg ccgtgatcgc ggtgtagatc
    11701 cggtcgggat cgagcggcga gcggccgcgg cggtggtaat ggcgcagc
//
LOCUS       DAOPCO010000110        11747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5389_length_11747_cov_4.604858, whole genome shotgun sequence.
ACCESSION   DAOPCO010000110 DAOPCO010000000
VERSION     DAOPCO010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_11747_cov_4.604858"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLS90_09140"
     CDS             complement(<1..868)
                     /locus_tag="PLS90_09140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02608.1"
                     /translation="MASPPPPPSLFVVTLEDLSRPTGQSLRLRALLPHLGRRFHLTLA
                     SCSETLPAEVAPHIAARWRLPLPAGARPLRWFLHPLGPWRMSRLRRALRRLPVEDSIL
                     YTDSILLAEMVRPRRAARAVVEVNGSITEEFRSRGLPLTGPIRRWLERWERRAFRLPD
                     HLIVAGAGLEQFVREFAGLAPGDRPAISVLGNGIDPARFHPGVDGARLRRRQEWGARP
                     VGIFHGWFRSYQGVDNLIRAVPHILRRRPDFLLVLVGDGPCRADAEALADRLGVRSSI
                     QFAGAVEAGEIAA"
     gene            complement(877..1926)
                     /locus_tag="PLS90_09145"
     CDS             complement(877..1926)
                     /locus_tag="PLS90_09145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02609.1"
                     /translation="MNQPELHPVEPEPASGDPPAIQLSVIVPMFNEEENVELTVNRLR
                     DTLRRFPDGPWEIILVNDGSLDRTMEIARELSRRPGYECVRVVGYRRNRGRGFALRTG
                     FAAARGRWVVSIDADLSYEPEYILRLTDVLREEEDIDMVLASAYMPGGGVVNAPLFRH
                     LVSRFGNVLLSWFMSSGRNKVHTITCVFRAYRREVLDDLELESEGKDIHLEILSKAMM
                     LGYRFREVPATLKWRTRGKSKFRFGTTALSHLIFALFERPILVFGLFGILLMLCSFGI
                     MGYLAVDYLRNGFLTEGRPMFTLVLLAFLGGLQLFAFGIIGTQFVTLRKEIVRMQRRL
                     RELTSRDRGRPAGSD"
     gene            complement(2239..3219)
                     /locus_tag="PLS90_09150"
     CDS             complement(2239..3219)
                     /locus_tag="PLS90_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02610.1"
                     /translation="MTKTPIAIVGLGKWGRNHVRVFAALPDCQVRWLFDLNDRAAAEQ
                     LALYPHLRAATSFDQILSDPAVQAIVIATTAGSHYQLAREALLAGKDVFVEKPMTLDL
                     AEGEELCAIAAERGRLIQVGHLLLFHPAVDYIKGLIDRNDLGEIYYIYSQRLNLGVVR
                     NDENALLSLAPHDISLINYLLGAAPIAAQAAGGCYLQDRIEDVIFVTLTYPQRKLAHI
                     HVSWLDPHKTRRLTIVGSRKMAVFDDTETTEKVRIYDKGVLRQEYENYGELLAVRTGD
                     ILIPNIPNTEPLKLQAQHFLECVRDRTPPRVDGRMGLEVVRVLAEASRRL"
     gene            complement(3375..5249)
                     /locus_tag="PLS90_09155"
     CDS             complement(3375..5249)
                     /locus_tag="PLS90_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HPK02611.1"
                     /translation="MSGSPHVATVVGARPQFIKLAMLTRRLAALTPVPFRHTLIHTGQ
                     HYDADMSEVFFRELELPRPRYELGVGSDRAAQQIAAIMSRLEPLLTADRPDALLVLGD
                     THSTLAAALVAAHLEIPLVHIEAGERIYRRRRVPEESNRVVADHLATLCLTASRPATW
                     FLEREGIAGRRVRFAGDLHYDLFLWAVGRPELRRVTSAALGLAPGGFILATLHRPENT
                     DAPERLAGLLRQLDEAPLPVVLPMHPRLRGRLADQAFTPKRALRLIDPVGYFDLLGLL
                     LDCRRVVSDSGGVIREAYFARRPCVVPMADNWWRQIAWLGWSIEVGDDEAKLRDALAD
                     FDPPATYPEGLFGDGNAAATIIDATAELIGSGAEPIWTPRGRPTSAPRAGAPGERTDF
                     TWAGYRRLIEGLRAEGYDLAPFPEAAARLSAARPFALLRHDVDMDLEAAVELARLEHG
                     WGVQSTYFIMVRNPFYSPFSAEGSRAVRQILDCGHHLGLHLEAAAYPADHPLHEACAA
                     EAGMLEAWFGRPVEIVSFHRPDARVLGGDPSLTAPRPHTYMALFRETIQYLSDSRGRW
                     REGHPLRHEAFHRRRPLHLLVHPIWYGAEPTGPHETLERFLDRHAARSRQALEANCRP
                     "
     gene            complement(5249..6286)
                     /locus_tag="PLS90_09160"
     CDS             complement(5249..6286)
                     /locus_tag="PLS90_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02612.1"
                     /translation="MQHPPSRRAADSPNPPSPGYPGYDEEVASRLTLLDFYHAWYGYR
                     FWFIAGFLIVAVGAFVWTKFFMPKFYRSEVRFFVGEKFTPESMIGLADIKLFTGSRND
                     QYQAVVHSNLAEQYLLSSDLITTVTWQLKHTPEEGQPPLDICQLLGIEEQDALVREAK
                     LVKLMRENLLQVRLSGTTGIVIYQVEMPTPMAAARYANASVRQLQRQMTQQAFSYWDE
                     ARRLYDQELERVMAERRELALRLDRLPPYDRYGPIEQERKALKEQLDVQAEQIALIRS
                     QVELLRLATEPTAKAAAQPVKVIDWGDPPIKKSRPKTILTTILAAGLYSFVFMLGLVV
                     AGFFTAQARRA"
     gene            complement(6311..7261)
                     /locus_tag="PLS90_09165"
     CDS             complement(6311..7261)
                     /locus_tag="PLS90_09165"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK02613.1"
                     /translation="MKMRRPVRLRLPLAILLLLVGTAVAQPDDDATTAALRIVPAAGD
                     ILEIRIGIDPAPTGEPYRILVGDQLSLVFHFGWPPADEPYPIQPGDEIKLYFRYSPEI
                     SQLYAQPEDDRIAVLSRPYVVEPDGKLWLAGLAEPVQVAGKTTTQFAADLRRLYQDQL
                     TSPQVQVSVTPMFEKQRTLKELFQAYEDRPVSFMEGRVTADGRIAAPLIPEITAAGKT
                     PAALSAELTAIYHELGYPWTSVTVIVGPAPEQRTPPLEALQPPNGRVAIAEDGTFLLP
                     FLPPQSVGDRSLAELAARLAEQYRLLGVPNPDVRIRRVRP"
     gene            complement(7385..8593)
                     /locus_tag="PLS90_09170"
     CDS             complement(7385..8593)
                     /locus_tag="PLS90_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPK02614.1"
                     /translation="MVSRVWLLLCLLAWSVAVPAADLSDVQLSEVFSGLTQPIFITHA
                     GDGRNDLYIVQQNGVIRVARGNQQNGFDLLPEPFHTFSVIENTDEKGLLGLAFHPDYV
                     NTGRLFVNLTRRVTGQLRTYVLELKRSGENYYQTDGSEKEILAFDQPQDNHNGGMMAF
                     GPEDDMLYISTGDGGAGGDIGEGHAPEGNGQSLATPLGKILRIDINPESGPYIIPGDN
                     PFIGMEGALPEIWALGLRNPWRFSFDRGGSHALFCGDVGQGDREEVDIIQAGGNYGWP
                     ILEGTRCNRPSQGCDNPDLIPPIAEYVNGGFQAVTGGYVYRGARIPSMQGLYIFGDYG
                     SGEIFYLEPDGQGGWQRPELFQDTSYLIASFGEDLNGELYLCHRGGRIYRFISTDPGN
                     TAARHAGRFH"
     gene            complement(8686..9540)
                     /gene="folP"
                     /locus_tag="PLS90_09175"
     CDS             complement(8686..9540)
                     /gene="folP"
                     /locus_tag="PLS90_09175"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPK02615.1"
                     /translation="MLWRCGRHQFDLSRRCLVMGVINVTPDSFSDGGRYFDAQVAADH
                     GSRLAEQGADILDIGGESTRPGAPPVPLDEELRRVIPVIEALRARLPRHALSIDTAKA
                     GVARAALAAGADIINDVTALTGDPAMLAVAAAADAGIVLMHMRGTPATMQQLTQYDDV
                     VREVGEYLRERLRASTAAGIDAERVVLDPGIGFAKSFEQNLELFQRLAELCDLGRPLL
                     IGASRKAFIGRLLGDAPPEARLEGTAAAVAAAVLAGARIVRVHDVAAMARVVRVTEAL
                     RPRRAIRG"
     gene            9823..10260
                     /locus_tag="PLS90_09180"
     CDS             9823..10260
                     /locus_tag="PLS90_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPK02616.1"
                     /translation="MKIFTIGFTRKSAREFFGLIRDSQARRLVDVRLHNVSQLAGFAK
                     KDDLAWFAREICGIDYVHLPELAPTEEILEAYRKNKRDWKTFEARFLALMKARKIEET
                     VPKKVLAEGCLVCSEDKPEHCHRRFVAEYLQAHWGNVEIIHLG"
     gene            10310..11296
                     /locus_tag="PLS90_09185"
     CDS             10310..11296
                     /locus_tag="PLS90_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02617.1"
                     /translation="MVPNADDPLERFTFEAARLMAEHPPVWRDRNKRIVYEIACPAGC
                     THAGTIAYSRWRAMAPPEAIHPAEAFRRVERRQDVYDYVPAVAGAMEWHVNFADPHLF
                     VAYGSGLFAQDEMQVAEHPVLGALREALDARGASALTEQRGQPTPVLVKGVERRCRIA
                     TDRNAEAGRPAGLYGNAFQAAAEEAIRRATTRIEPPTITNLIAMAAPAGGDGRYDEEE
                     IERILTTAHTGFRAAVLESGGAPAVVHTGFWGCGAFGGNRQLMALLQVLAAGMAGVAR
                     LVFHTFDAAGNAAFDAAAGRAREALGAGEIPTRDLIRRIAEMGFEWGVSDGN"
     gene            11322..11615
                     /locus_tag="PLS90_09190"
     CDS             11322..11615
                     /locus_tag="PLS90_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02618.1"
                     /translation="MATTKTLISPAENAILEGNYTLAMIRNGAAVFNVLWRLDSDSDG
                     MTDDWKSNYRHGQEGIGANPRCPTEAQPNELDRDSERPADDAALADLVNEEAG"
BASE COUNT         2005 a         3855 c         4097 g         1790 t
ORIGIN      
        1 acgcggcgat ctcgccggcc tcgaccgcgc ccgcaaattg aatactggat cgaacgccga
       61 gtcggtcggc gagcgcttcc gcgtcggctc ggcacggccc atcgcccacc aggacgagca
      121 gaaaatcggg tcgccggcgg aggatgtgcg gcacggcgcg gatcagattg tcgacgccct
      181 ggtagctgcg aaaccagccg tggaagatgc cgacgggccg cgcgccccat tcctggcgcc
      241 gccggaggcg ggcgccgtcg actccgggat ggaaccgcgc gggatcgatc ccgttgccga
      301 gcaccgagat ggccggccgg tcgcccggcg ccaggcccgc gaactcccgt acgaactgct
      361 cgaggcccgc gccggcgacg atcagatgat cgggcagccg gaacgcccgt cgctcccagc
      421 gttcgagcca gcgtcggatg ggcccggtca gcggcagacc gcggctgcgg aattcctcgg
      481 tgatgctccc gttgacctcc acgaccgcgc gggcggcgcg ccgcggccgg accatttcgg
      541 ccagcaggat ggaatcggtg tagaggatcg agtcttccac cgggagccgc cgcagcgccc
      601 ggcgcagccg cgacatgcgc cacggtccca acgggtgcag gaaccagcgg agcggccgcg
      661 cgccggccgg cagcggcagc cgccaccgcg cggcgatatg cggcgccacc tccgccggga
      721 gcgtttccga acacgaggcg agcgtgagat gaaagcgccg ccccagatgc ggcagcagcg
      781 cgcgcaaccg cagcgactgg ccggtcgggc gcgacagatc ctccagcgtg acgacgaaga
      841 gcgagggcgg aggagggggc gaagccaagg ttggagtcaa tcggacccgg cggggcgacc
      901 ccgatcgcgg ctggtcagtt cgcgcaggcg gcgctgcatg cgcacgatct ccttgcgcag
      961 ggtgacaaac tgcgtgccga tgatcccgaa ggcgaacagt tgcaggccgc ccaggaacgc
     1021 cagcaggacg agcgtgaaca tcgggcgtcc ttcggtcaga aacccgttgc ggaggtaatc
     1081 cacggccaga taccccatga tcccgaacga gcagagcatg agcagaatgc cgaacagacc
     1141 gaagacgagg atcggccgct caaagagggc gaagatcagg tgcgagaggg cggtcgtccc
     1201 gaaccggaat ttggacttgc cgcgcgtgcg ccatttgagc gtcgcgggca cctcgcggaa
     1261 gcggtagccg agcatcatcg ccttggagag gatctccaga tgaatatcct tgccttcgga
     1321 ctccagttcc agatcgtcga gcacctcgcg ccggtaggcc cggaacacgc aggtgatcgt
     1381 atggacttta ttcctgccgg acgacatgaa ccaactgagc agcacgttgc cgaagcggct
     1441 gaccaggtgg cggaagagcg gcgcattgac cacgccgccg ccgggcatat aggccgaggc
     1501 gaggaccatg tcgatgtcct cctcctcgcg caggacgtcg gtcagccgca ggatgtattc
     1561 gggttcgtaa ctcagatcgg cgtcgatgct gacgacccag cggccgcgcg cggcggcgaa
     1621 cccggtgcgc agcgcgaaac cgcgcccgcg attgcggcgg tagcccacga cccgcacgca
     1681 ttcatagccg ggccggcgcg acagttcgcg cgcaatttcc atcgtccggt ccaggctgcc
     1741 gtcgttgacg aggatgatct cccacgggcc gtcgggaaac cgccgcagcg tgtcgcgcag
     1801 gcggttcacc gtcagctcga cattctcctc ctcgttgaac atcgggacga tgacggacag
     1861 ctggatcgcg ggagggtcgc cggacgcggg ctccggctcg accgggtgca gctcgggctg
     1921 attcatcccc tcgccgccgg ggctgggggg cggcccggac agcggccggg cctcatcgga
     1981 aggctgggcg gtggcgagcg ccggctcctc aagttcggtc cgggcctttg cgtttccgga
     2041 gcgttccgcg cgcggcgctg ggacatcgag gcggcgcctg ctcggccgca aaccctgaaa
     2101 cagcgtggcg aagggcaagg tcggaaagat attgaacaag cgatgctccc cccatgcggc
     2161 ccattgggct tcgtggcggg gtccgctctc ccccggctcc tgcctcttcc gtgcgaacgc
     2221 cgccagatag ggaacagtct ataaccgtcg gctcgcttcg gcaagcacgc gaacgacttc
     2281 caggcccatc cggccgtcga cgcgcggggg cgtccggtcg cgcacgcact caaggaagtg
     2341 ctgcgcctgc agcttgagcg gctcggtgtt ggggatgttc gggatcagga tgtcgcccgt
     2401 gcgcaccgcc agcaactcgc cgtaattctc gtattcctgg cgcagcacgc ccttgtcgta
     2461 gatccgcact ttttcggtgg tttcggtgtc gtcgaagacc gccatcttgc gcgagccgac
     2521 gatcgtcagc cgccgcgtct tgtgcgggtc gagccacgag acgtgaatat gcgccagctt
     2581 gcgttgcgga tacgtcagtg tcacgaagat cacgtcttcg atccggtcct gcaggtagca
     2641 gccgccggcg gcctgggccg caatcggcgc ggcgcccagc aggtagttga tcagcgagat
     2701 atcgtgcggc gccaggctga gcagggcgtt ctcgtcgttg cgcacgacgc ccaggttcag
     2761 ccgctgactg taaatgtagt aaatctcgcc cagatcattc cggtcgatca ggcccttgat
     2821 gtagtccacg gcggggtgaa acaggaggag atgccccacc tggatcagtc gtccgcgctc
     2881 agcggcgatc gcgcacagct cttccccctc ggccagatcc agcgtcatcg gtttctcaac
     2941 gaacacgtcc ttgccggcca ggagcgcttc gcgggcgagc tggtagtgcg aaccggccgt
     3001 ggtggcgatg acgatcgcct ggaccgccgg atcgctcaga atctggtcga acgacgtcgc
     3061 cgcccgcaga tgcgggtaaa gcgcgagctg ttcggcggcg gcccggtcat tcagatcgaa
     3121 gagccagcga acctggcaat cggggagtgc ggcgaagacg cggacgtgat tccgccccca
     3181 cttgcccagg cccacaatcg cgatcggtgt tttggtcatg ctccgcggcc tgtcgctgcc
     3241 gaatccagcc agaaaggcga ttgaaccgga atccccctcc ccgcgcaacc gcaatcaggg
     3301 ggggctgaag cgacaaaaag gggggcaagg gacgccatcg acaaggtggg cgtgagaggc
     3361 tgatgcgtga gcggctacgg ccggcagttg gcctccagcg cctgccggga tcgggcggcg
     3421 tggcggtcca gaaaacgctc cagcgtctcg tgcggaccgg tcggctccgc gccgtaccag
     3481 atcggatgga ccagcaggtg cagcgggcgg cggcggtgga aggcttcgtg gcgcagcgga
     3541 tgcccctcgc gccagcggcc gcgcgaatcg ctcaagtact ggatcgtctc gcgaaaaagc
     3601 gccatgtaag tgtgcgggcg gggcgccgtc agcgacggat ccccccccag gacgcgcgcg
     3661 tcggggcggt ggaagctgac gatctcgaca ggccggccga accatgcttc gagcatcccc
     3721 gcttcggccg cgcacgcctc gtgcagcgga tgatcggcgg gataagccgc cgcttcgagg
     3781 tggagcccaa ggtggtggcc gcaatccagg atctggcgaa ccgcgcgcga cccctcggcc
     3841 gagaacggcg aatagaacgg attgcgcacc atgatgaagt atgtggactg gacgccccag
     3901 ccgtgttcga ggcgcgccag ctcaacggcc gcttccagat ccatgtcgac gtcgtggcgc
     3961 agcagcgcga acggccgcgc ggccgacaat cgcgcggcgg cctcgggaaa cggggcgagg
     4021 tcgtaaccct cggcgcgcag gccctcgatc agccggcgat accccgccca ggtgaaatcg
     4081 gtccgctcgc cgggcgcgcc cgctctgggc gccgacgtcg gccttccccg tggagtccat
     4141 atcggttcgg cccccgaacc gatcagctcg gcggtggcat cgatgatcgt cgctgcggcg
     4201 ttgccgtcgc cgaacaaccc ctcgggatag gtcgcggggg gatcgaaatc ggccagcgcg
     4261 tcgcgcagct tcgcctcatc gtcgccgacc tcgatcgacc agcccaacca ggcgatctgc
     4321 cgccaccagt tatcggccat cgggacgaca cagggccgcc gggcgaagta ggcttcgcgg
     4381 atgacgcccc ccgaatccga gaccacgcgc cggcaatcga gcaacaggcc gagcagatcg
     4441 aaatagccga cggggtcgat caggcgcagc gcgcgcttcg gggtgaatgc ctgatcggcg
     4501 aggcggccgc gcagccgcgg gtgcatcggc aggacgaccg gcagcggcgc ttcgtcgagc
     4561 tggcgcagca ggccggccag ccgttccggt gcgtcggtgt tttccggtcg gtgcagcgtg
     4621 gccagaatga agccgcccgg cgcgagccca agcgcggcgg atgtgacgcg gcgcaattcg
     4681 ggccttccga cggcccagag gaagaggtcg taatgcaggt cgccggcgaa gcgcacacgc
     4741 cggccggcga tcccctcgcg ctccagaaac cacgtcgccg ggcgcgacgc cgtcaggcac
     4801 aatgtcgcca ggtgatcggc gacgacgcgg ttggactcct ccggcacgcg ccgccgccgg
     4861 tagatccgct cgcccgcctc gatgtggacg agcggaatct ccagatgcgc ggcgaccagc
     4921 gccgccgcca gcgtggaatg cgtgtcgccc agcaccagca gcgcgtcggg ccggtccgcg
     4981 gtcagcaagg gttccagccg gctcatgatc gcggcgatct gctgcgcggc ccggtccgac
     5041 ccgacaccca gctcatatcg cggccgggga agttccagct cgcgaaagaa gacttcgctc
     5101 atgtcggcgt cgtagtgctg gccggtgtgg atcagcgtgt ggcggaacgg cacgggcgtc
     5161 agcgccgcca gccggcgggt gagcatcgcc agcttgatga actgcgggcg cgcgccaaca
     5221 acggtggcga catggggcga cccgctcatc acgcgcggcg ggcctgggcc gtgaagaagc
     5281 cggcgaccac cagccccagc atgaagacga agctgtagag gccggcggcg aggatcgtcg
     5341 tcaggatcgt tttgggccgg ctcttcttga tcggcggatc gccccagtcg atgaccttga
     5401 ccggctgggc ggccgccttg gccgtcggtt cggtcgccag ccgcagcaac tcgacctgcg
     5461 agcggatcag cgcgatctgt tcggcctgca cgtcgagctg ttccttgagc gccttgcgct
     5521 cctgctcgat cgggccatag cgatcgtacg gcggcaaccg atccagccgc agcgccagct
     5581 cgcggcgctc cgccattacc cgctccagct cctgatcgta gagccggcgc gcttcatccc
     5641 agtaggaaaa ggcctgctgg gtcatctggc gctggagttg ccggacactg gcgttggcat
     5701 agcgcgcggc ggccatcggc gtcggcattt cgacctgata gatcacgatg cccgtcgtgc
     5761 ccgacagccg cacctgcagc aggttttcgc gcatcagctt gaccagtttg gcctcgcgga
     5821 caagcgcgtc ctgctcctcg atgccgagca actggcagat gtccagtggc ggctgacctt
     5881 cttccggggt gtgcttcaac tgccacgtca ccgtggtgat cagatccgac gagagcaggt
     5941 attgctcggc gaggttggag tgaaccacgg cctgatactg gtcgttgcgg ctgccggtga
     6001 agagcttgat gtcggccagg ccgatcatcg attcgggcgt gaacttctcg ccgacgaaga
     6061 agcggacctc gctgcggtag aacttcggca tgaagaactt ggtccagacg aacgcgccga
     6121 cggcgacgat cagaaacccc gcgatgaacc agaaccgata gccataccag gcgtggtaga
     6181 aatccagcag ggtcaggcgc gatgcgactt cctcatcata ccccggatac cccggcgagg
     6241 gagggttggg gctgtccgcc gcgcgacgtg aagggggatg ctgcatggag cgggtcgatc
     6301 tccttgtcga tcaggggcgg acccgtcgga tccgaacatc cgggttcggc acgccgagca
     6361 gccggtattg ctccgccagg cgcgcggcca gttcagcgag cgaccggtcg ccgaccgact
     6421 gcggcggcag gaacggcagc aggaaggtcc catcctccgc gatggcgacg cgaccattgg
     6481 ggggctgcag cgcctcgagc ggcggcgttc gctgttcggg cgccgggccg acgatcaccg
     6541 tgaccgaggt ccaggggtag cccagttcgt gatagatcgc ggtgagctcg gcgctcaagg
     6601 cggcgggggt tttgccggcg gcggtgatct cggggatcag cggcgcggcg atccgaccgt
     6661 cggccgtcac ccgcccctcc atgaaggaga cgggccggtc ttcgtaggcc tggaacagct
     6721 ccttgagcgt ccgctgcttc tcgaacatcg gcgtcaccga gacctgcacc tgcgggctgg
     6781 tcaactggtc ctgatacagc cggcgcaggt cggctgcgaa ctgggtcgtg gttttccccg
     6841 ccacctgcac cggctcggcc agaccggcca gccagagctt gccgtcgggt tccaccacat
     6901 acgggcggct caggacggcg atgcgatcgt cctccggctg cgcgtagagc tggctgattt
     6961 cgggactgta gcggaaatac agcttgatct cgtcgcccgg ctggatcggg taaggctcgt
     7021 ccgcgggcgg ccagccgaag tgaaagacca gcgagagctg atcgccgacg aggatgcgat
     7081 agggctctcc ggtgggcgcc ggatcgatcc cgatccggat ctcaaggatg tcgccggcgg
     7141 ccgggacgat gcgcagcgcg gccgtcgtcg catcgtcgtc gggttgcgcg acggcggtcc
     7201 cgacaaggag gagcaggatt gccagcggca accgcaatcg aaccggacgt cgcatcttca
     7261 tcggctgaag ttcccccgtc atgcggaccg cggccgcgcg cgggcgctgc ccgtccctgt
     7321 tcccaccgga caggggtagt ttgcacgact gcacggccgg gcgcaagatt ggcgcttgac
     7381 gggtttaatg gaaccggccg gcatggcgcg ccgccgtatt gcccgggtcg gtgctgatga
     7441 agcggtagat ccggccgccg cggtggcaga gatagagctc cccattcaga tcctcgccga
     7501 acgaggcaat cagataggac gtatcctgga acagctcggg ccgttgccag cccccctgcc
     7561 cgtcgggttc cagatagaag atctcgcccg agccgtagtc gccgaagatg tagaggccct
     7621 gcatcgaggg gatgcgcgcg ccgcgataga cgtaaccgcc ggtaacggcc tggaagccgc
     7681 cgttgacata ctcggcgatg ggagggatca ggtcggggtt gtcgcaaccc tggctggggc
     7741 gattgcagcg cgttccctcc aggatcggcc agccgtagtt gccgccggcc tgaatgatgt
     7801 caacctcttc gcggtcgccc tggccgacat cgccgcagaa cagggcgtgg ctgccgccgc
     7861 ggtcgaacga gaagcgccac ggattgcgca ggccgagggc ccagatttcg ggcaacgcgc
     7921 cttccatccc gatgaacgga ttgtcgccgg ggatgatata gggcccgctt tcgggattga
     7981 tgtcgatccg cagaatcttg ccgagcgggg tcgccagcga ctggccgttg ccttcggggg
     8041 cgtggccttc gccgatgtcg ccgcccgcac cgccgtcgcc ggtcgagatg tagagcatgt
     8101 cgtcctccgg gccgaaggcc atcatcccgc cattgtggtt gtcctgcggc tggtcgaacg
     8161 cgaggatctc cttctccgag ccgtcggtct ggtagtagtt ctcgcctgaa cgcttcagct
     8221 cgagcacgta ggtgcgcagt tggcccgtca cgcggcgggt caggttgacg aacagccgac
     8281 ccgtgttgac gtaatcgggg tgaaacgcca gcccgagcag gcccttctcg tcggtgttct
     8341 caatgacgct gaaggtgtgg aacggttcgg gcaggaggtc gaagccgttc tgctggttgc
     8401 cccgggcgac gcggatgacg ccgttctgct ggacgatgta gagatcgttt cgcccgtcgc
     8461 cggcgtgggt gatgaagatc ggttgcgtga gcccggaaaa gacttcgctg agctgaacat
     8521 cggagagatc cgccgccggc accgcaacgg accaggccag cagacacaac aacaaccata
     8581 ctctggaaac catccggata cacctttgcg ggggggtcgg ggccgcccgt cggcggacgc
     8641 gcgcgaaatt gatttagcca agtttacagt gcgcgagcgg aaccgtcacc ctcggattgc
     8701 gcgccggggg cgcagcgcct cggtgacccg caccacgcgg gccatcgcgg ccacgtcgtg
     8761 cacccggacg atccgcgccc cggccaggac cgccgcggcg acggcggccg ccgtcccctc
     8821 cagccgcgcc tcgggcgggg cgtcgcccaa caggcgcccg ataaacgcct tgcgcgacgc
     8881 gccgatcagc agcggccgcc ccagatcgca caattccgcc agccgctgaa acagttccag
     8941 gttctgctcg aacgacttgg cgaaaccgat ccccgggtcc agcacgacgc gttcggcgtc
     9001 gatgcccgcc gcggtcgagg cgcgcagccg ttcgcgcaga tactccccca cttcgcgcac
     9061 gacgtcgtcg tattgggtca actgctgcat cgtcgccggc gtgccgcgca tatgcatcag
     9121 gacgatgccg gcatcggcgg cggcggcgac ggcgagcatc gccgggtcgc ccgtcagcgc
     9181 cgtcacgtcg ttgatgatgt cggccccggc cgccagcgcc gcgcgggcga cgcccgcctt
     9241 ggccgtgtcg atcgagagcg cgtggcgcgg caggcgcgcg cgcaaggcct cgatgaccgg
     9301 gatcacgcgg cgcagttcct catcgagggg gaccggcggc gcgccgggcc gggtggattc
     9361 gccgccgatg tcgaggatgt cggcgccctg ctcggccagc cggctgccgt gatccgccgc
     9421 cacctgggcg tcgaagtagc gtccgccgtc gctgaacgaa tcgggcgtga cgttgatcac
     9481 gcccatcacc agacagcgcc ggctcagatc gaactggtgg cggccacagc gccagagcat
     9541 gcacggtccc ctcgcgaaga atctgccggg ggaaacagtc tgccgccgcc gtccggcgcg
     9601 cgcaacgggt aatcaccggc aaagacgcgg gcccaactcc ggtgatgtct tcttctgtta
     9661 tttccgtgta tgatgtctgt ttcagccgtc ggatggccgc gatctcggcg tttggttcgg
     9721 gcaagcgtcg agcgcgaccc ggggaggaag caacggcgcg acggatcgcg ccagaggccg
     9781 gtttcagcca gccgacaggg gacgagcgga aggaggaggc ggatgaagat cttcaccatc
     9841 ggatttaccc ggaagagcgc ccgggagttt ttcgggctga ttcgcgacag ccaggcccgg
     9901 cggttggtcg acgtgcggct gcataacgtc tcgcagctcg cggggttcgc caagaaggac
     9961 gacctggcct ggttcgcccg ggaaatctgc ggcatcgact acgtccatct gcccgagctg
    10021 gcgccgaccg aggagatcct ggaggcctac cggaagaaca agcgcgattg gaaaacgttc
    10081 gaagcccggt ttctggcgct gatgaaggca aggaagatcg aggaaaccgt gccgaaaaag
    10141 gtgctcgccg agggatgcct cgtctgcagc gaggacaagc ccgagcactg ccatcgccgc
    10201 tttgtcgcgg agtatctgca agcccattgg gggaatgtcg agatcatcca tctgggttaa
    10261 gggcggccac agttcggttg cgaatctgca tgaaaggagc gcattcaccg tggtccccaa
    10321 cgctgacgac ccactcgaac ggttcacctt cgaggccgcg cggctgatgg cggaacatcc
    10381 gcccgtctgg cgcgaccgca acaagcgcat cgtttacgag atcgcgtgcc cggccggctg
    10441 cacgcacgcc ggcacgatcg cctattcgcg ctggcgggcg atggcgccgc cggaagcgat
    10501 ccatccggcc gaggcctttc gccgcgtcga acggcgccag gacgtctatg actacgtccc
    10561 cgccgtggcg ggcgcgatgg agtggcacgt caacttcgcc gacccgcacc tgttcgtcgc
    10621 ctacggctcc ggcctgttcg cccaggacga gatgcaggtg gccgagcatc cggtgctggg
    10681 ggcgctgcgc gaggcgctcg atgcccgggg cgcgtccgcg ctgaccgagc aacgcggcca
    10741 gccgacgccg gtgctggtca agggggtgga gcggcgctgc cggatcgcga ccgaccggaa
    10801 cgccgaggcg ggccggcccg ccgggctcta tggcaacgcc ttccaggcag ccgcggagga
    10861 ggccatccgc cgggcgacga cccgcatcga gccgcccacg atcaccaacc tgatcgcgat
    10921 ggccgccccc gccggcggcg acggacgtta cgacgaggag gaaatcgagc ggatcctgac
    10981 cacggcgcat accggatttc gggcggcggt cctggagtcg ggcggcgcgc cggccgtcgt
    11041 ccacaccggg ttctggggct gcggcgcgtt cggcggcaac cgccaactga tggccctgct
    11101 ccaagtgctg gcggccggga tggccggcgt cgcgcggctg gtgttccaca cgttcgacgc
    11161 ggcggggaac gcggcgttcg atgccgccgc cggccgggca agggaagcgc tgggcgcggg
    11221 cgagatcccg acccgcgacc tgatccgccg catcgcggag atgggattcg agtggggcgt
    11281 cagcgacggc aactgagcgc cgctccgctg gcgtggggtg tatggcgacg acgaaaaccc
    11341 ttatttcccc ggccgagaac gccatcctgg aggggaatta cacgctggcc atgatccgca
    11401 acggcgcggc tgtcttcaat gtgctctggc gcctcgattc cgacagcgac gggatgacgg
    11461 atgattggaa atcgaactat cgccatggcc aggagggcat cggagccaac ccccggtgtc
    11521 cgacggaagc gcaaccgaac gagttggacc gcgactccga acggccggca gatgatgccg
    11581 cccttgcgga tctggtgaac gaggaagctg gatgagtcgg gacgatgcca aaatcccatg
    11641 ccactcaaac agagaagccg ccttgcggcg gctcccaagt caagaaaact gagtggtgga
    11701 cctggacggg atcgaaccga cgacctccgc attgcgaacg cggcgct
//
LOCUS       DAOPCO010000111        11709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5409_length_11709_cov_4.715892, whole genome shotgun sequence.
ACCESSION   DAOPCO010000111 DAOPCO010000000
VERSION     DAOPCO010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11709
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5409_length_11709_cov_4.715892"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="PLS90_09195"
     CDS             <1..912
                     /locus_tag="PLS90_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02619.1"
                     /translation="AAAQGIDLRDYHDLIALGVDHRVLSPHFRRWLNWRTASPALRHY
                     LAHSQGGPAARMEGESLREWLWLAWLEGQSEAEAIGAACQSGGALGRPHMVGLVGPTG
                     GGETTSLAKLSSIIRHEKRQNPVIVTLDAQRFGATEQWRKLARLMGIEIQEAVTQEDV
                     RACMETWDRFDWVGIDTPGGMPPDSPVGRLYGSIVAQCPDLNTLLVMPTTQHESAAAS
                     AMRRARDFGARGIVFTKLDETERHGGIVNLTMDGTWNIAGFATGQRVPQDWVPASSAT
                     LWERVLAPAAAGDAALGAHRLTGGVRA"
     gene            909..1796
                     /locus_tag="PLS90_09200"
     CDS             909..1796
                     /locus_tag="PLS90_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPK02620.1"
                     /translation="MSVLTTSTIRRPRIQAAAGRPIVRHVPHVITVTSGKGGVGKTNL
                     TANLGLLLREGRRRVMVLDADLGLANIDVILGLHPDFNLSHVLSGEKRLGEILTRAPG
                     GLRVLPASSGISSITDLSDAQKIRLLQELEGLQEDFDYLLIDTGAGISNNVIYFNLAA
                     QTIVVVLTPEPTSLTDAYALIKVLSRDYHQRRFKVLANDVRTEAEGLEVFEKLTRVAD
                     RFLNVSLDYLGHVLHDRQLRESVRMQRPFVEAFPDGPASASLRKVAKQITAMPHNPMD
                     SDLGLLWRNLLGGPAAAGH"
     gene            1911..2795
                     /locus_tag="PLS90_09205"
     CDS             1911..2795
                     /locus_tag="PLS90_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HPK02621.1"
                     /translation="MSGAVTYAPAKGRRRKIQTAAPAVAPVQAEDAAPPVKPKAKVKT
                     PRTAYAEAAKTSLEARNEMIVEYLPLVKFVASRIASRLPSHIELNDLINSGIIGLIDA
                     IEKFDASRKIKFKTYAEFRIKGAILDELRALDWVPRSTRQKASRLERAYAELEQMLGR
                     AASDAEMMEFLGISLEEFDQLVREARGVALISLDELQGGDHDENFERNLLEYLADPEH
                     MSPAQVMNLDQVYKIVADAIDQLPEKERLVISLYYYEELTMKEIGEILSITESRVSQI
                     HTKAILRLRGRLTKLLGD"
     gene            2902..4161
                     /locus_tag="PLS90_09210"
     CDS             2902..4161
                     /locus_tag="PLS90_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK02622.1"
                     /translation="MAIVRLQPEMITPAASLPSLFASLLKITEALGSSLELHTVLQTI
                     LEQTTQEMQAQQGSILLFDEHQDRLQMLASIGLPEEMVRKGYIARRGSIAEYVIEHNQ
                     PLILNDQGGGNEYRPLDNTRRIVSSMCVPLRARGRVLGTINLNRLDPELGPFHDADLE
                     TMVLLTSHAAIFIENSRLHEANLRSERLAAIGQTVAGISHCIKNILTGVRGGLSLIDM
                     ATGSQNWEMLGKGRDILGRNVERISSIMLDMLDYSKERTPAKARLPLNSLIEEVFNTV
                     RGEAAQREIALESQVDADAAFVDGDGQQLFRCLLNLVGNALDATPKGGRVWVTAETAT
                     APAALNRLRGGDATAAAVIRVCDSGPGIAAENHTTIFEPFFSTKGSKGTGLGLAVTRK
                     LIREHGGDIELESAPGESAIFAIYLPA"
     gene            complement(4286..5104)
                     /locus_tag="PLS90_09215"
     CDS             complement(4286..5104)
                     /locus_tag="PLS90_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPK02623.1"
                     /translation="MNRERKIGWGILAAAALAAVIAVGMFLAHESASLYFTLVFDDAK
                     GLQAGDRVQLNGVDIGVVKVVQLHTDPSPRVDVRVKIEPEHAEKVRADATAIISSTSF
                     PNVSGQKVVEIHNSETQPPAPPMQDDAVVYGQNGALGLQAWKLKQSLSSGADSLMKQV
                     GALGEQAAELAAELSEMATSPQVQEAIGELRGFLATMKEEGKQALAELEAKWPELKRQ
                     IEPVLNELRDFGREYIAEQIRALIEEIERTLRAWRGEMEPADSTPAPAVEGEVT"
     gene            complement(5346..5657)
                     /locus_tag="PLS90_09220"
     CDS             complement(5346..5657)
                     /locus_tag="PLS90_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02624.1"
                     /translation="MYSAISSLSSGLQHAERGFASAARTVAYAPATGEEQIRAVDGAE
                     ANSRAAIDSHRRDPRQAVFRRREAEAAAEVIRRQRAFESSAVALRRLDQTVGTLLDVE
                     A"
     gene            6209..9442
                     /gene="carB"
                     /locus_tag="PLS90_09225"
     CDS             6209..9442
                     /gene="carB"
                     /locus_tag="PLS90_09225"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPK02625.1"
                     /translation="MPKRSDFHKVLIIGSGPIVIGQACEFDYSGTQACKALRELGYEI
                     VLVNSNPATIMTDPGMADATYIEPLNVATLTRIIERERPDALLPNLGGQTGLNLCAEL
                     ARAGVLARHGVQVIGVEVEAIERGEDRIAFKATMNALGIDLPRSEPAYSVDDAERIAA
                     ELGYPVVIRPAYTLGGTGGGLVYNQEELRVVASRGISASMVGQILVEESVLGWEELEL
                     EVVRDARGQMITVCFIENIDPMGVHTGDSFCAAPMLTIDPALQRRLQDYAYRIVEAIR
                     VIGGTNVQFAHDPATGRIIVIEINPRTSRSSALASKATGFPIALISAKLACGLTLDEI
                     PYWREGTLDKYTPSGDYVVIKFARWDFEKFKGAEDRLGTQMRAVGEVMSIGRNYKEAF
                     QKAIRSIEKGRYGLGFARDFHHKTREELLALLGWPTSERQFIMYEALRKGATIDELHQ
                     KTRIKPWFIQQMRELVELEEQILACNGVELPDELLIRAKRDGFADRYLARLLDLPEAE
                     LRTRRLRLGIAEAWEPVPVSGVDEAAYYYSTYNAPDRSRAGGGRKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALREAGVQSIMVNCNPETVSTDYDTSDKLYFEPLTVEDVLSIHQK
                     ERPDGVIVQFGGQTPLNIAGELAAAGVKILGTPPATIDLAEDRDQFRRMMQQLGIPMP
                     EAGMAASLDEARALAARIGYPLMVRPSYVLGGRGMEVVDDEAMLEAYVAAAIDVSPER
                     PILLDKFLVNAIEAEADAICDGIDAFVPAVMEHIELAGIHSGDSACVIPPISIPAKHL
                     DTIDEYTRRIAMELGVIGLMNVQYAICDDVVYVLEANPRASRTVPLVSKVCNIPMARL
                     ATQVMLGRRLADLGLRPRPVPHFGVKEAVFPFNMFPEVDPVLGPEMRSTGEVLGLAES
                     FGLAYFKAQAATQRPLPTHGTVLITVAEHDRPEAVEVARRFRQLGFHIKATGGTRAWL
                     AEHGIEAEAIDKLHEGRPNIVDGIMNGEIDLVINTPAGRRSQHDDSYIRKSAIRYKIP
                     YITTMAGALAAAKGIAAWSESGETIKSLQDYHADLAGARPEHASLAS"
     gene            10193..10834
                     /locus_tag="PLS90_09230"
     CDS             10193..10834
                     /locus_tag="PLS90_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK02626.1"
                     /translation="MQRLQTQDTESIKVALPQEIVRFKESRYGHRLPGVLLVRAGKSC
                     DEAVDLLSHSPRTIQYWVESFEQSGWAGLQDNDRPGRPIAIDERTRQPVVQDLRLSPR
                     EWGYEQNLWGGKLLLHHLARRDDTHLGVRQCQRLFHELGFCRWPPIDDAGRHVRRTKL
                     SGLPRAAAAPSPAWSPTGVAPDMRNYLKRFVCLRLQRAITRPIHNLSSQKGTE"
     gene            10834..11424
                     /locus_tag="PLS90_09235"
     CDS             10834..11424
                     /locus_tag="PLS90_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02627.1"
                     /translation="MFKIRAFTLIELLIVVAIIAILAAIAVPNFLEAQSRAKVSAAKS
                     DIRAMVTGLEAYRVDNTQYPGDFEHFTGYLVARNSPDKVWDYSRTLQRITTPIAYLTE
                     LPSSTPFGPASSAHWLEIHGYMYQGGDWWWRAINEGNYKAYYNEQYWDAKYLLSCMGP
                     AKSFGTFIPYDPTNGTISLGDIIYVGGNSASYHSFN"
BASE COUNT         2137 a         3782 c         3802 g         1988 t
ORIGIN      
        1 gcggccgccc agggcatcga cctgcgggac tatcatgatc tgatcgcgct gggcgtggat
       61 caccgggtgc tgagccccca ctttcgccgg tggctcaact ggcgcacggc ctcgcccgcg
      121 ctgcggcact acctggccca cagccagggc ggccccgccg cgcggatgga gggcgagtcg
      181 ctgcgcgagt ggttgtggct ggcctggctg gagggccaga gcgaagcgga ggcgatcggc
      241 gcggcgtgcc agagcggagg ggcgctgggc cggccgcata tggtcggtct ggtgggtccg
      301 acgggcggcg gagaaacgac gtcattggcc aagctctcgt caattatccg tcacgaaaaa
      361 cgtcagaatc ccgtcattgt gacgctcgac gcgcagcggt tcggcgccac ggaacagtgg
      421 cgcaagctcg cccgcctcat gggaatcgaa attcaggaag ctgtaactca agaagatgta
      481 agggcctgca tggagacctg ggaccgcttc gactgggtcg ggatcgatac ccccgggggg
      541 atgccgcccg actccccggt cggccggctc tatggctcaa tcgttgctca atgccccgac
      601 ctgaacacgc tgctggtcat gccgaccacc cagcacgaaa gcgccgccgc cagcgccatg
      661 cgccgggcgc gcgatttcgg cgcccgggga attgtgttta ccaaactgga cgagaccgaa
      721 cgccacggag ggattgtgaa tctgacgatg gatggaacct ggaacatcgc cggcttcgcc
      781 acgggacaac gggtgcccca ggattgggtg ccagccagtt ccgccactct ttgggaacgg
      841 gtgctggcgc cggccgccgc cggcgatgcc gcgctgggcg cgcaccggtt gaccggcggg
      901 gtgcgggcat gagcgtgctg acgacttcaa ccattcgccg cccccgcatt caggccgccg
      961 ctggccgccc catcgtgcgg cacgtccccc acgtgatcac cgtcaccagc ggcaaggggg
     1021 gcgtcggcaa gaccaacctg acggccaatc tgggcctgct gctgcgcgag gggcgccgcc
     1081 gcgtgatggt cctcgacgcc gacctgggcc tggccaacat cgacgtgatc ctcggcctgc
     1141 atcccgattt caacctgagc cacgtgctga gcggcgagaa acggcttggc gagatcctga
     1201 cgcgcgcccc cggcggcctg cgcgtcctgc ccgccagcag cggcatcagc tcgatcaccg
     1261 acctgagcga cgcgcagaag atccgcctgc tgcaggaact ggaggggctg caggaggatt
     1321 tcgattacct gctgatcgac accggcgccg ggatttccaa taacgtgatc tatttcaacc
     1381 tggcggcgca gacgatcgtc gtcgtgctga cgccggagcc gacgtcgctg accgacgcct
     1441 acgcgctgat caaggtcctg tcgcgcgact accaccagcg ccggttcaag gtgctggcca
     1501 acgacgtgcg gaccgaggcc gaagggctgg aggtctttga gaagctgacg cgcgtcgccg
     1561 accggtttct gaatgtttcc ctcgactacc tgggccacgt gctgcacgac cgccagttgc
     1621 gcgagtcggt gcgcatgcag cgcccgtttg tcgaagcgtt tcccgacggg ccggccagcg
     1681 ccagcctgcg gaaagtggca aagcagatca ccgcgatgcc gcacaacccg atggacagcg
     1741 atctgggact gctctggcgc aatctgctgg gcggaccggc tgccgcgggt cactgaacgg
     1801 gagcccggct cccctggggc gtgacgccca tgcaacacac cacggatagc ccagccggac
     1861 gggacaggaa gtcagacgcc gggccggcca cgatcaggag gatggataca atgagtggag
     1921 cagtcaccta cgcgcccgcc aaggggcggc gccgcaagat ccagacggcg gcgccggcgg
     1981 tcgcgccggt tcaggcggag gacgccgcac cgcccgtcaa gcccaaagcc aaggtcaaga
     2041 cgccgcgcac ggcctacgcc gaagcggcca agaccagcct tgaggcgcgc aacgagatga
     2101 ttgtggagta cctgccgctg gtcaagttcg tggccagtcg catcgccagc cggctgccca
     2161 gccacatcga gctgaacgac ctgatcaact cggggatcat cggcctgatc gacgcgatcg
     2221 agaagttcga cgccagccgc aagatcaagt tcaagactta cgccgaattc cgcatcaagg
     2281 gggcgatcct ggacgagctg cgcgcgctgg actgggtgcc gcgctcgacc cgccagaagg
     2341 ccagccggct ggagcgggcc tacgccgaac tggagcagat gctgggccgg gcggcctccg
     2401 acgccgagat gatggagttc ctcggcatca gcctggagga attcgaccag ttggtgcgcg
     2461 aagcccgcgg cgtcgccctg atcagcctgg acgaactcca gggcggcgac cacgacgaga
     2521 acttcgagcg caacctgctg gaatacctgg ccgaccccga acacatgagc cccgcccagg
     2581 tgatgaacct ggaccaggtc tacaagatcg tggccgacgc catcgaccag ttgcccgaga
     2641 aggaacggct cgtcatttcg ctctattact acgaagaact gacgatgaag gaaatcgggg
     2701 agatcctctc gatcaccgaa agccgggtct cgcagatcca caccaaggcg atcctgcggc
     2761 tgcgcggccg gctgaccaaa ctgcttgggg actaaagaat tggttcggat cagtcgaaac
     2821 gagagtgtgg accgtacccg ctgccgggga atggaaactc gacgggccgc gacttcccgc
     2881 cgctgactgg gaccgataac catggcgatt gtccgtctgc aacccgagat gatcacgccc
     2941 gcggcgtcgc tcccctcgct gttcgcctcg ctgctcaaga tcaccgaagc gctcgggtcg
     3001 agcctggaac tgcatacggt gcttcagacg atcctggagc agacgactca ggaaatgcag
     3061 gcccagcagg gatcgatcct gctcttcgac gagcaccagg atcggctgca gatgctggcc
     3121 agcatcggcc tgcccgagga gatggtgcgc aagggctaca tcgcccgccg cggctcgatt
     3181 gccgagtacg tgattgaaca caatcagccg ctgattctga acgaccaggg gggcggcaac
     3241 gagtatcgcc cgctggacaa cacgcgccgg atcgtctcct cgatgtgcgt gccgctgcgc
     3301 gcgcgcggcc gcgtcctggg cacgatcaac ctgaatcggc tcgatccgga gctcggtccg
     3361 ttccacgacg ccgacctgga gacgatggtg ctgctgacga gtcatgccgc catcttcatc
     3421 gaaaactcgc gcctgcacga agcgaacctg cgcagcgagc ggctcgccgc gatcggccag
     3481 accgtcgccg gcatcagcca ctgcatcaag aacatcctga ccggcgtgcg cggcggcctt
     3541 tccctgatcg acatggcgac cggcagccag aactgggaga tgctgggcaa ggggcgcgac
     3601 atcctcggcc gcaatgtcga gcgcatcagc tcgatcatgc tcgacatgct cgactattcg
     3661 aaggagcgca cgccggcgaa ggcccgcctg ccgctcaact cactgatcga ggaagtgttc
     3721 aacaccgtgc gcggcgaggc ggcgcagcgc gaaatcgcgc tcgaaagcca ggtcgacgcc
     3781 gatgccgcct tcgtcgacgg tgacggtcaa cagcttttcc gctgcctgct gaatctggtg
     3841 ggcaatgcct tggacgcgac gcccaagggc ggccgcgtgt gggtgacggc cgagaccgcg
     3901 acggcgccgg cggcgctcaa ccggctgcgc ggcggcgacg cgaccgcggc ggccgtgatc
     3961 cgcgtctgcg attccgggcc gggcatcgcc gccgaaaacc acaccacgat tttcgaaccg
     4021 ttcttctcga ccaagggttc caaggggacc ggcctggggc tcgccgtcac gcgcaagctg
     4081 attcgcgagc acggcggcga catcgaactg gaatccgccc ccggcgaatc ggccatcttc
     4141 gccatctacc ttccggcctg acacataaca tcgtccctcg tcgtcgtgat cgacgatcag
     4201 ggaatggacg caagggacat caaggaccta cgggacacaa cggacgcgcg tccgtccccc
     4261 tcgtcctggc tcttcactct tttcctcaag tcacttcgcc ttcgaccgcc ggggcgggcg
     4321 tggaatcggc cggttccatt tcgccgcgcc aggcccgcag cgtccgctcg atttcctcga
     4381 tcagcgcgcg gatctgctcg gcgatgtatt cgcggccgaa gtcgcgcagt tcgttgagca
     4441 ccggctcgat ctggcgcttc agttcgggcc acttcgcctc cagctcggcc agcgcctgct
     4501 tgccctcctc cttcatcgtg gcgaggaacc cgcgcagttc gccgatggcc tcctgcacct
     4561 gcgggctcgt cgccatctcg ctcagctcgg cggccagttc ggcggcctgc tcgcccagcg
     4621 cgccgacctg tttcatcagc gaatcggcgc cgctgctgag cgactgtttc agtttccacg
     4681 cctgcaagcc gagcgcgccg ttctggccgt agacgacggc gtcgtcctgc atcggcggcg
     4741 ccgggggctg cgtttcgctg ttgtgaatct cgaccacctt ctgccccgag acgttgggga
     4801 acgaggtgct ggagatgatc gccgtggcgt cggcgcgcac cttctcggcg tgctccggct
     4861 cgatcttcac gcgcacgtcg acccgcggcg acgggtcggt gtgaagctgg accaccttga
     4921 cgacgccgat atcgacgccg ttgagctgga cgcggtcgcc cgcctgcaag cctttggcgt
     4981 catcgaagac cagcgtgaag tagaggctgg ccgactcgtg ggccagaaac atgccgacgg
     5041 cgatcaccgc cgccagcgcc gccgcggcga ggatgcccca gccgatcttg cgctcgcgat
     5101 tcatcctccc ccgcctcccg ccgtgcgaat cttcccgggg ctggcggccc cgcgcggcca
     5161 tgaactccgg gctggcttca ctccgatgcg gcctcggttc ccatcctgac ccagacgcgc
     5221 gagcgggtca atggcctccc tcacgagcgg caggcggaaa tccgtcggat ccgaccgatc
     5281 cgacggattt cgcggctttc atgtgacaat gttgattcgc ggaatgaaaa agcggagcga
     5341 gcgcttcacg cctcgacgtc gagcagcgtc ccgacggtct ggtcgagccg gcgcagcgcc
     5401 acggcggacg attcgaacgc gcgctggcgg cggatcactt cggcggcggc ttcggcttcc
     5461 cgccggcgga agacggcttg acgggggtcg cgccggtggg agtcgatggc ggcgcgcgag
     5521 ttggcctcgg ctccgtccac cgcgcgaatc tgctcctcgc ccgttgccgg agcatacgca
     5581 accgtgcggg ccgccgaggc aaagcctcgt tcggcatgct gcaacccgga ggagagcgag
     5641 gagatcgcgc tgtacacgac cgacccccgt tcacggcgcc ggaatcggct cggcgagcgt
     5701 tccggcggat tgacggaagg gacggggcct cctcctgagt ctatcccccc tggtccgtct
     5761 atcggcggtt gcgggaggcg acttgagcgg aaaattgatt ttttttgggg gaagctcaac
     5821 gaatggaaga ccagcggttt tcgagagccg aggacgagag ccgaatttgg gggtcggccg
     5881 ttgggggcga tcacgaactg cccatgaaca gcggttcgca acgaaaatga ggggtgaatg
     5941 gccgattcga acgatccagg ccttgccggt tcgatcagca gggcagtccc gtcagggacg
     6001 gcagacttca gcccgctact tcagtggcgg gaaccgggat gcccatagcg acagagtccc
     6061 gcagggacgg cagaacctct ggaatcgatc acgaggacaa gggccgaggg acgggatgag
     6121 agacgggcaa tggattcaga gttaggttga tcgggcgccc tgctttcagg tataactatc
     6181 cgatttcgtc gggggggtta cgccatcgat gcccaagcgc agcgacttcc acaaggttct
     6241 gatcatcggc tccggaccga tcgtcatcgg ccaggcctgc gaattcgact actcgggcac
     6301 gcaggcctgc aaggcgctgc gtgaactcgg ctacgaaatt gtgctggtca attccaatcc
     6361 ggccacgatc atgaccgatc ccggcatggc cgacgcgacc tatatcgagc cgctcaacgt
     6421 ggccaccctg acccggatca tcgagcggga acgccccgac gccctgctgc ccaatctggg
     6481 cggccagacc ggtctgaatc tgtgcgccga actggcccgc gccggcgttc tggcgcgcca
     6541 cggcgtccag gtgatcggtg tggaagtgga ggcgatcgag cggggcgagg atcggatcgc
     6601 cttcaaggcg acgatgaacg ccctggggat cgacctgccg cgcagcgaac cggcctattc
     6661 cgtcgacgac gccgagcgga tcgccgccga gttgggttac cccgtcgtga tccggcccgc
     6721 ctacacgctg ggggggaccg gcggcggcct ggtttacaac caggaggagc tgcgcgtggt
     6781 cgccagccgg gggatctcgg ccagcatggt cggccagatc ctggtcgagg aatcggtgct
     6841 gggctgggag gaactggagc tggaagtcgt ccgcgacgcc cgcggccaga tgatcaccgt
     6901 ctgttttatc gaaaacatcg atccgatggg ggtgcacacg ggcgattcgt tctgcgcggc
     6961 gccgatgctg acgatcgacc cggcgctcca gcgccgcctg caggattacg cctaccggat
     7021 cgtcgaggca atccgcgtga tcggggggac gaacgtccag ttcgcccacg atccggcgac
     7081 gggccgcatc atcgtgatcg agatcaaccc gcgcacctcg cggtcgtcgg cgctggcctc
     7141 caaggcgacc gggttcccta tcgcgctgat ctcggcgaag ctggcctgcg gcctgacgct
     7201 cgacgaaatc ccctactggc gcgaggggac gctcgacaag tacaccccgt cgggcgacta
     7261 cgtcgtgatc aagttcgccc gctgggactt cgagaaattc aagggggccg aggaccggct
     7321 cggcacgcag atgcgggccg tcggcgaggt gatgagcatc ggccgcaact acaaggaggc
     7381 gttccagaag gccatccgct cgattgaaaa ggggcgctac ggcctggggt tcgcccgcga
     7441 tttccaccac aagacgcgcg aggaactgct cgccctgctg ggctggccga ccagcgagcg
     7501 ccagttcatc atgtatgagg cgctgcgcaa gggcgcgacg attgacgaac tgcatcagaa
     7561 gacccggatc aagccctggt tcatccagca gatgcgcgaa ctggtcgaac tggaagagca
     7621 gattctggcg tgcaacgggg tcgagctgcc cgacgaactg ctgatccgcg ccaaacgcga
     7681 cggcttcgcc gaccgctacc tggcgcggct gctggacctg cccgaggccg agctgcgtac
     7741 gcgccggctc cggctgggga tcgccgaggc gtgggaaccg gttccggtca gcggcgttga
     7801 cgaggcggcc tactattact ccacctacaa cgcgccggac cggtcccggg ccggcggcgg
     7861 gcgcaaggtg atggtcctcg gcggcggacc caatcgcatc ggccagggga tcgagttcga
     7921 ctattgctgc gtccacgcgg cgttcgcgct gcgcgaggcg ggtgtgcaat cgatcatggt
     7981 caactgcaat ccggaaacgg tctcgaccga ctacgacacc tccgacaagc tgtatttcga
     8041 accgttgacc gtggaggacg tcctcagcat tcatcagaag gagcggcccg acggcgtcat
     8101 cgttcagttc ggcgggcaga cgccgctcaa tatcgcgggc gagttggcgg cggccggcgt
     8161 gaaaattctg ggcaccccgc cggcgacgat cgacctggcc gaggaccgcg accagttccg
     8221 ccggatgatg cagcagctcg gcatccccat gcccgaggcc gggatggccg cttcgctcga
     8281 cgaggcccgc gccttggccg cgcgcatcgg ctatccgctg atggtccgcc cctcctatgt
     8341 gctgggcggg cgcgggatgg aggtggtcga cgacgaagcg atgctcgagg cgtatgtcgc
     8401 cgcggcgatc gacgtcagcc ccgagcggcc gatcctgctc gacaagtttc tggtcaacgc
     8461 catcgaggcc gaggccgacg cgatctgcga cggcatcgat gccttcgtgc cggcggtgat
     8521 ggagcacatc gagctggccg gcattcattc gggcgattca gcctgcgtga tcccccccat
     8581 cagcatcccg gccaagcatc tcgacacgat tgacgaatac acccgccgga tcgcgatgga
     8641 actgggcgtg atcggtctga tgaacgtgca gtatgcgatc tgcgacgacg tggtctacgt
     8701 gctcgaagca aacccgcggg cgtcgcgcac ggtgccgctc gtctccaagg tgtgcaacat
     8761 cccgatggcg cggctggcga cgcaggtgat gctgggccgg cggctggccg atctgggcct
     8821 caggccccgg ccggtcccgc acttcggcgt caaggaggcg gtcttcccgt tcaacatgtt
     8881 cccggaggtc gatccggtgc tggggcccga gatgcgctcg acgggcgagg tgctggggct
     8941 ggccgagtcg ttcgggctgg cgtatttcaa ggcgcaggcg gcgacccagc gcccgctgcc
     9001 gacgcacggg acggtgctga tcaccgtggc cgaacacgac cggccggagg cggtggaagt
     9061 ggcgcgccgt ttccgtcagt tgggattcca catcaaggcg acgggcggca cgcgggcctg
     9121 gctcgccgag catggcatcg aggccgaagc gatcgacaag ctgcacgaag ggcggcccaa
     9181 catcgtcgac ggcatcatga acggcgagat cgatctggtg atcaacacgc cggcggggcg
     9241 ccgcagccag cacgacgact cctacatccg caagagcgcg attcgctaca agatccccta
     9301 catcacgacg atggccggcg cgttggcggc cgccaagggg atcgccgcct ggtccgaatc
     9361 cggcgagacg atcaaatcgc tgcaggacta ccacgccgat ctcgccggcg cccggcccga
     9421 gcacgcgtcg ctggcgagct gaggggccgt cctcattcaa gaattcggct ctcgtcctcg
     9481 tcctcgtatc gagctgttcc gccgcccctg cggacctctt tcgttattgt ttccgatttc
     9541 ccgccacgga agtcgcgggc tgacatctgc cagccctgac cggactggcc gcccgaccga
     9601 tccggcaagg cctcggccgt tgaaatcggt ccttcaccgc cccccatacc cattcctcat
     9661 tgtgaaccac cggttcacga gcaatccgat caccttggcc tccacccctc ggcatctcgg
     9721 cgccgcggcg tgcgatgatt tcttcaattg gagcatgcgt gtcggtctcg gtttcggaat
     9781 tcgtgccatt cgtgcttctc cctttatgtt tcttccctcg attttcaatc gaagtgaagt
     9841 cggcccctgg ctaccgtctg gaccagcgtc tccagccccc caatacgaac agaactgcga
     9901 tgtgccccaa aaacagcaac aggtccataa aacagcactt gacagattgg cgaaagtagt
     9961 gctaaatcca tttaacacgc tgagaactgc tcgaccgaac tgagagctgc ttgaccgaaa
    10021 tgggaccttg tttatcgtga acgcggaggt ccagccattt gcgtttcgca ggaggatagg
    10081 gggcgatgag ggagtgcatc agttggctcc tacttctata tgggacgaca taagtaattg
    10141 cgcatagctg cgtggtcggc tctacccttg attcaattca agggggtgga ccttgcaaag
    10201 acttcaaacc caagataccg agagcatcaa ggtggccttg ccgcaggaaa tcgtccgatt
    10261 caaggaatcg cgctatggcc atcgcctgcc tggggtgctg ctggtgcgcg cgggcaagtc
    10321 ctgcgatgag gcggtcgacc tgctgagcca cagtccgcgc acgatccagt attgggtgga
    10381 atccttcgag cagagcggct gggccggact ccaagacaat gaccgaccgg ggcgtcccat
    10441 cgcgattgac gagcgaaccc gccaacctgt ggtgcaagac ctgcgcctgt cgccgcgcga
    10501 gtggggctac gaacagaatc tttggggcgg caaactgctc ttgcatcacc tggcccggcg
    10561 cgatgacacc catctaggtg tgcgccagtg ccaacgactg ttccacgaat tgggcttctg
    10621 ccgctggccg cctattgacg atgccggccg tcacgttcgg cgcaccaagc tttcaggcct
    10681 tcctcgggca gctgctgcgc cgtcgccggc atggtcgcca acaggagttg cgccggatat
    10741 gcgcaattat ttaaaacgct ttgtatgcct gcgcttacag agggctatca cccgcccgat
    10801 acacaactta tcatcacaga aaggaactga ataatgttca agattagagc ttttacactg
    10861 atcgagctgt taatcgtcgt agcgatcatt gcgatcttgg cggccatcgc tgtacccaac
    10921 ttcctggaag ctcagagtcg ggccaaggtc tcggcggcca aatccgatat ccgggcgatg
    10981 gtgaccggct tagaggccta ccgagtcgac aacacccaat atccggggga tttcgagcat
    11041 ttcacggggt atctcgtggc ccggaattct ccagataagg tgtgggatta cagccgcacg
    11101 ctccagcgca tcactacccc gattgcctac ttgaccgagc ttccctccag cacgcccttc
    11161 ggtccggcct ccagtgctca ctggctggag atacacggct acatgtatca gggcggggac
    11221 tggtggtggc gggcgatcaa cgagggcaac tataaggctt attacaacga acagtattgg
    11281 gacgcgaagt acctgctcag ttgcatgggc ccggccaaga gcttcggcac tttcatccct
    11341 tacgatccga ccaacggcac gatcagtctt ggggacatca tctacgtcgg aggcaatagc
    11401 gcttcgtatc attcctttaa ttgaggggcg gcttgtccgg tgcggcgccg gggaataatg
    11461 gatgggggtc tgcttccacg cgctgcatgt gcggcgcacg catggccgcg gatgagcggc
    11521 ctatagtttc gcggccgact tggacgggag caaaatcgat gcgcccgtgg gacatcagac
    11581 cgtcccgagt cgcattgggc actatttcca gcggctggat ccgtcccgcc ccgggcccgg
    11641 ccgggcaatt tcgaaaatgt cgtggcgact gataagagcg gataacgcaa aatacccgca
    11701 catgggacc
//
LOCUS       DAOPCO010000112        11701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5416_length_11701_cov_4.521467, whole genome shotgun sequence.
ACCESSION   DAOPCO010000112 DAOPCO010000000
VERSION     DAOPCO010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5416_length_11701_cov_4.521467"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLS90_09240"
     CDS             complement(<1..246)
                     /locus_tag="PLS90_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008315861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HPK02628.1"
                     /translation="MNPPIPSLQFLDWLVIVGYLFLTIGLGLYFTRRAGRSMDAFFVS
                     GRSLPWYLAGVSMIATSFASDTPLWISSLVRQYGIYYI"
     gene            815..1942
                     /locus_tag="PLS90_09245"
     CDS             815..1942
                     /locus_tag="PLS90_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK02629.1"
                     /translation="MSDRPPKTPRAAHRRASSNLAPVKTPATLAEVARLAGFSTATVS
                     RAFNFPDKVKPEVRQRILDVAKLVRYRPNRLVSGVLHGRVPILGVVLNVLNTAFMPEL
                     LGGVFEACETAGYSCMVYDSAARQEREVNALKLCIQHRLSGIILFPVDNESLERSQIL
                     TEIGELDVPVVTLMSPIAALPEAPLVGNDDYKGALAAVGHLIELGHRRILHLSGSDAH
                     YRSSDERLRGYRDAYTRAGLAVPRDLVRIAGFGPGMQTWRILEELEFKGKTRPYTAIF
                     AASDYVANRAIQFFQSRGIQVPQQVSIVGFGDLPLCELTWPPLTSVNQDFHRVGVMAA
                     QVCLDRVKRKPTNEASPWLVPVSLIVRESTGPIARIRKRPS"
     gene            complement(2145..2849)
                     /locus_tag="PLS90_09250"
     CDS             complement(2145..2849)
                     /locus_tag="PLS90_09250"
                     /inference="COORDINATES: protein motif:HMM:NF027338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HPK02630.1"
                     /translation="MGEMQSPSEQVIQYTIRRAEHPPDLTSGWDSDPWRRGDVLAVTH
                     FHAQSGTHRPITRTRVLYDDKGLSLFFQVIDRYVRCLWTRYQDPVCEDSCVEFYVQPK
                     PDRGFFNFEMNCGGTLMVSYSTADGSDWSRNRTVASVPFELGRQVKIHHTLPARVEPE
                     LTEPTLWELSCFIPFSLLEHYVGPLGKLHGQCWRANFFKCGDHTSHPHWAAWSPIGPE
                     LDFHQPRYFGTVIFEG"
     gene            complement(3025..3831)
                     /locus_tag="PLS90_09255"
     CDS             complement(3025..3831)
                     /locus_tag="PLS90_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02631.1"
                     /translation="MEQLSMQRQVCTWDDFRQHWAGFLNFRIDGECRFPDFPFPPIDQ
                     VVDEVRRDPDSRIWRGAAGDSLDKTDIAEEFRAMSLAQAMESRFQMSHFKLNNFFGPG
                     QLLHRFDTDCLIPWLDRLKEAGFTFERYYPIFFISGRQCSTNFHIDPTNVVAWQIYGT
                     KFFHGTQDPERWAPYEMRMNWRDTRRPPGITEADLLTYRMSPGDMLWNAMLTPHWVEA
                     GDDEVALSMNLAIDGLRLNGELCPHGRELREYMIAQGLDVDLKGKAPGDY"
     gene            complement(3925..4887)
                     /locus_tag="PLS90_09260"
     CDS             complement(3925..4887)
                     /locus_tag="PLS90_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK02632.1"
                     /translation="MRLVVIGGTGHTGTYLIPRLVEAGHEVIQISRGQQHPYLLHAAW
                     AHVTQIAADREAEDRAGTFGERVRALEPDVVIDMVCFTPESVRSLVEALRGCVQHFLH
                     CGTIWVHGPAAEVPTTEAAPRRPFGDYGIRKAEIEVYLLREARLTGFPATILHPGHIV
                     GPGHWPLNPAGHFNPEIFQRLARGEEVALPNLGMETVHHVHADDVAQAFMQALTHRSV
                     ALGESFHVVSSAALTLRGYAEAVASWFGRPARLRFVPWEEWKEAAAPDDARFTWDHIA
                     HSPNCSIEKARRLLNYQPRYSSLQAVFEALSWMIDHGKLVCDRQ"
     gene            complement(4962..5381)
                     /locus_tag="PLS90_09265"
     CDS             complement(4962..5381)
                     /locus_tag="PLS90_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02633.1"
                     /translation="MWYRWMLIAFLTNGFSQFGARILQDYGLAETHAYLYLSFWYGTG
                     LLIALATFLPLRQRIRPREILIGGIMGLCSSTGWFLLTGALGQGIPGYLAFPVAVGGS
                     LSVVALVGIVILRERLSAYGYAGVLCGIAAIIVLTTA"
     gene            complement(5403..5954)
                     /locus_tag="PLS90_09270"
     CDS             complement(5403..5954)
                     /locus_tag="PLS90_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02634.1"
                     /translation="MSVGMLMLVIALLVLAGLGITHKLADLRDCRPSAVNVSLFFWAA
                     VVMWSYTLFVKVLGQGESLFPPFTRSAVIVAIICGTCASFGILTFQIGVRFGPITTSW
                     LIVNLSTLIPAVLSLIIYQEWKQEIKWQHPVSLLLVLGSVFLLWRDKVVLARLAPVAV
                     AAAPGAPATVKSQATAGRAPGRS"
     gene            complement(5965..7017)
                     /locus_tag="PLS90_09275"
     CDS             complement(5965..7017)
                     /locus_tag="PLS90_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK02635.1"
                     /translation="MNGRERIRNLLEGRPVDRTPVLASTLANAAWLRKIPQGKFHGDA
                     HTLARTVIDVSDQMGLDGIYISSDNWILHEALGGALILPEDDEPRGVKHHLLDSIDEL
                     TGLRVPDPQRDGRMPMMLEAARKAVELADGRLFVEANIDSGPFQLALTLLGSEQAIMV
                     ALREPERFHDLLEFNTQVAIAYGYAMAQCGVDAIQFGESSASLVSSELYREMVLPYDQ
                     RLIAALKAEGVKVFLHVCGNSTHLLDDLEASGADCFELDAMVDLDVAFARIGNRIVLR
                     GNVDTLLLLNGPVEEIHAAAARCMEKARAGGGRLILSPGCGVPKYTPAHHVAALVTAA
                     TRQDADCLPVGSVDRK"
     gene            complement(7062..8927)
                     /locus_tag="PLS90_09280"
     CDS             complement(7062..8927)
                     /locus_tag="PLS90_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02636.1"
                     /translation="MMIRYTGFALRTLILGALMLLMAAPALAVDTLTIANARSDQFAL
                     ADRAVIVAAEAAKVPAERLKALGQRDANVSFDLLTTGSTLPVDKLPIYLGTKADLNSF
                     ATDVPSLVKDARLDAEGFVIATRGDSPGIWIIGADDSGLRYGVGEFWNYHAQIDREGI
                     TVPANLHVERSPMFSKRILWNWNFMTNWNEDIPSIHQTRMVDPNGTLEPYLEQPDGYL
                     NQFQRVVDYAADHKLNGLIIWGFINDSHGGVESAQRLSSYARRNAVRILPGIGTVIYG
                     GFYHGGDSPWSLTTWLREHPDVRRMVGKDGQPIDAPCPSDPALQQFLREGSEWFFTTF
                     PDIGGVNIEHGDFFECQCDRCQAERAKPGNDPNFLWDMMVTQLPVIETGLRINPSIWF
                     TYSPYWGYNREMMENPPSFLRQYPEQAIVQWTYSGMIGSPAGWPADLKPPVGATHSIG
                     LLHQGSYWSQPRQWWGSPGQTYALIPDIIQQTCARAIEDASEGVVIVGQVGAASPQND
                     LNYLAFEDFTWNPRLSWDDWFANRLPAIYGSEQLARRFYELAGDETQSIEALESAREE
                     ALELATELEDPRQIRRWKNLAEELRRRLALVNAGMTQPYGPGPLEGIEDVQLDYP"
     gene            9412..>11701
                     /locus_tag="PLS90_09285"
     CDS             9412..>11701
                     /locus_tag="PLS90_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02637.1"
                     /translation="MNRTLNCWLVFALALVIRPLGAEQLPAWDAPQFMTLIEQGRPQG
                     AIILADQATATEEIAARKLAEWLGEQSGVEFPILRTREMDAHSTGNFVLLGTPQNQPL
                     LAELVIEGSGGAADLPFLDDEGFGVETFSRDGRNYLLVGGRTPAGVFHGAIYARDFLF
                     DILHPVDQIVVRDVELVRNPALLERGPYLLPQYGVVPEYTLEHWKHIIDRMAEGGLNR
                     IYWWVAGMYTSPRFPETFTITNSKMGVEDINTLIDYAHERGMKFLVGGGAFFWHGVDG
                     YLEDHPELAATSSAGMCPSNPEARRFMQAYSLEWLETFPKADGLWLEPRDEAGACFCE
                     DCQVRLDAFNSRAYGQNEISFLKDLMKQVWDRNPAHTLVWLIEFLEHKDFFPHLDDPL
                     YFERIREIQDSRVKWMVVWEAFNLPGPGNDRRPVPFFTRNGLHWDKPYWPNLQNVFDH
                     ARICAEQGYLGYSNAWEIGFASNDWYIHDVPYPVDIIPEVVTRLAFREACWAPEQSWD
                     AFTERVHRYFFSREAPRQLAEDILWLRQYITAANHEFRSPMEFDTETALADEVRRVDQ
                     LEDRTEKASALERLSALVDILEETRAEALPRMLEIEARLAEIEPQASRKTLQTLAIMR
                     RAIDDSRAVYATAVPDDATLDAALAAVARMKEGAREEDARRDAATDVYLTEHGRAVAH
                     VYAPFDNEWAGRRLAGRLKQWTGSDLTLQCAGAKPEGSGDLIAIGTVESNPVVREVLG
                     ENPRLEGLGEEGYILQTIRWKDR"
BASE COUNT         2433 a         3431 c         3602 g         2235 t
ORIGIN      
        1 gatgtaatag atgccatatt ggcgcaccaa ggaggaaatc cacagcgggg tgtctgaggc
       61 gaaactggtg gcaatcatcg agacgccggc gagataccag ggcagcgaac gacccgatac
      121 aaagaacgca tccatgctcc ggccggcccg ccgggtgaag tacaggccca atccgatcgt
      181 caggaacagg tagccaacga tcaccagcca gtccaggaat tgcagggaag gtattggtgg
      241 attcataact agtcagttcc ttggcggtgg cgccttcgag cgtgaatggc aaccagataa
      301 atcgactaac gggaggggcg acccaatctg atctgtggag caggtcgaaa cgggaaaaag
      361 acccgatata tccatatctg aggttcgggc ataccctgca atcttcgaca aaggccaggg
      421 cttcatgtca tggcgatcgt tggcgctcga tcactcttgc ctctcgatcc cgagagctgc
      481 taagggcttg caaagccagc agcaactgtg tgtgaatcct tcctggagat tcgacctgtg
      541 aacgctcggc catgccgtga caagcttcct ggaaggctgg gtccgacgtt agtgccaatc
      601 aggatccggc cacgtcaaac caatgtattc cgttctcaac gaaaggccgt gcgctgtgct
      661 gctgggtcag gatggtaaac cgttgtaata ctcaatgcaa gtggaaatgt aatctgttac
      721 aagaagcctt ggatttgagc aggcgatgcg ccatgctggg gttgtcctca tccctgccgc
      781 tcgccgacgc ggccggccaa ggtgaaagga ttcgatgagc gatcggccac caaagactcc
      841 gcgcgcggcg catcgacgcg ccagttcaaa cctggctccc gtcaagacgc ccgccacctt
      901 ggccgaagtc gcccgcctgg cgggattctc gacagccacg gtttcgcgcg ctttcaattt
      961 ccccgacaag gtcaagcctg aggtgcgtca gcgcatcctg gacgtggcca aactggtccg
     1021 ctatcggccc aaccgcctgg tcagcggcgt gctgcacggg cgtgtcccca tcctcggcgt
     1081 cgtccttaac gtcctgaaca ccgccttcat gcccgagctg ctgggcggcg tgttcgaagc
     1141 ctgcgaaaca gccggctact cctgcatggt ctatgactcc gccgcgcgcc aggagcggga
     1201 agtcaacgcc ctcaaacttt gcatccagca tcgcctgtcg ggcattatac tttttcctgt
     1261 cgataatgaa tcgctcgaac gcagccagat actcactgag attggcgaac tggatgtgcc
     1321 ggtcgtgacg ctgatgtcgc ccatcgccgc cctgcccgaa gccccgctcg ttggcaacga
     1381 cgattacaag ggggccttgg cggccgtcgg gcacctcatc gagttgggcc accgccgcat
     1441 cctgcatctg agcggatcgg acgcccacta ccgctcatcg gatgaacggc tgcgaggcta
     1501 tcgagatgcc tacacccgcg ccggactagc ggttcccagg gatctggtgc ggatcgccgg
     1561 ctttggcccc ggcatgcaga cctggcgcat cctggaggag ctggaattca aggggaaaac
     1621 gcgcccgtac accgcgatct tcgccgccag cgactatgtc gcgaaccgtg ccattcagtt
     1681 ctttcagagc agagggattc aggtgcctca gcaggtgtcg attgtcggtt tcggcgacct
     1741 gccgctttgc gagctgacct ggccgccgct gacctccgtc aatcaggatt tccaccgtgt
     1801 tggcgtgatg gccgcccagg tctgcctcga tcgggtaaag cggaagccaa ccaacgaagc
     1861 atcgccctgg cttgttcccg tgagcctgat cgtgcgcgag agcaccggcc cgatcgcccg
     1921 aatccgcaag cgaccctctt gaatcagcct tattcctcaa ttcaagcgca tctcaacgcg
     1981 cttatcgccc ccgcgccgtt cagcggtgaa taagttccta actgagcctc ctgcaaaagt
     2041 ccaatttccc gcgagagccg gtacagaaag agccaaggca tggagcgagg cccggcatcc
     2101 tgaccagcat ggatgggggg cccgagacgg cgaaagcggg ggtgttatcc ctcaaagatg
     2161 accgtgccaa agtacctggg ctgatggaaa tcgagctctg gcccgatggg cgaccaagcc
     2221 gcccaatggg gatgggaggt gtgatcacca cacttgaaaa aattcgcccg ccagcactgc
     2281 ccatggagct tcccgagcgg tccgacataa tgttccagta aggagaatgg gatgaagcag
     2341 cttaattccc aaagggttgg ctccgtaagt tccggttcga ccctcgccgg caaggtgtga
     2401 tgaatcttca cctgtcggcc caactcaaaa ggcacgctcg cgaccgttcg attgcggctc
     2461 caatcgctcc cgtcggcggt tgaatacgac accatgagcg ttccaccgca attcatctcg
     2521 aagttaaaga aacctctatc cggcttgggt tgcacataga actcgacgca gctgtcttcg
     2581 caaaccggat cctgatacct ggtccataaa cagcgaacat agcggtcaat cacttgaaag
     2641 aaaagggata ggcccttgtc gtcgtagaga acccgcgtcc gagtaatcgg ccgatgagtc
     2701 ccgctttggg catgaaagtg ggtcaccgcg agcacatccc ctcggcgcca gggatcggag
     2761 tcccatccac tggtcaggtc agggggatgt tccgcgcgac gaatcgtgta ttggatcacc
     2821 tgttcggatg gagactgcat ttcgcccatg tggacatcct gccggtgagg ctcctccagt
     2881 cgagggtaca acgcgaactg attagtcgga caggtcccgg ttgtggggac tgttggggtt
     2941 gggggacgag cacaatcccg ctccgcgcac ttcgggcctc tgacgatgct attggacggt
     3001 caggagagaa actcatttgg tctgtcagta atcgccggga gcttttccct tgagatccac
     3061 gtccaagcct tgggcgatca tgtactcccg caactctctc ccgtgggggc agagctcgcc
     3121 gttgaggcgc agtccgtcga tggcgaggtt catgctcaag gcgacttcat catcgccggc
     3181 ttcgacccaa tgcggcgtca gcatggcatt ccaaagcatg tcgccaggag acattctgta
     3241 ggtgagcaag tccgcttcgg tgatgccggg cggacggcgc gtatcgcgcc agttcatgcg
     3301 catctcgtag ggcgcccagc gttcgggatc ctgggtgcca tggaagaact tagtgccata
     3361 gatctgccac gcgacgacgt tggtcggatc gatatgaaaa ttggtggaac actgccgccc
     3421 actgatgaag aagatcgggt aatagcgttc gaaagtaaag ccggcttcct tgagacgatc
     3481 gagccagggg atcaggcaat ccgtatcaaa ccggtgaagc agctgaccgg ggccgaagaa
     3541 attgttcagt ttgaagtgag acatctgaaa ccggctttcc atggcctgcg cgagggacat
     3601 ggcacggaac tcctcggcaa tgtcggtctt atcgagcgaa tcaccggcgg cgccgcgcca
     3661 gatgcgtgag tccgggtcgc gacgcacctc atcgacaacc tgatcgatgg ggggaaaggg
     3721 gaagtcggga aagcgacatt cgccatcgat gcggaaattg aggaaacccg cccaatgctg
     3781 ccggaaatca tcccaagtac acacctggcg ttgcatgctt agctgttcca tggtcgaggc
     3841 atctcctggt atctgtcaaa tgagtaacag gccgtcggag gatcgaaagc ggaacaagga
     3901 gggaatcaag cgacaaggcg aatttcattg cctgtcgcac accagttttc cgtgatcaat
     3961 catccatgac agcgcttcga acacggcctg cagcgagctg taacgcggct ggtagttcaa
     4021 cagtcgccgg gctttttcga tgctgcagtt cggactgtgg gcgatgtggt cccaggtgaa
     4081 ccgggcatcg tcgggtgcgg ccgcctcttt ccattcctcc caggggacga accgcagccg
     4141 ggcgggccgg ccgaaccacg aagccactgc ctcggcgtag ccacgaaggg tgagcgccgc
     4201 ggatgaaacg acatggaagc tttcgcccag agcgacactg cggtgggtca gggcctgcat
     4261 gaaagcctgg gcgacatcgt ccgcatgaac gtgatggacg gtttccatgc ccaggttggg
     4321 caaagcgacc tcttcccccc gcgcaagccg ctgaaagatc tcggggttga aatgtccggc
     4381 cgggttaagt ggccagtgcc ccggcccgac gatatgcccc ggatgcagga tcgtggccgg
     4441 gaaccccgtc agccgcgcct cgcgcaagag ataaacttca atctctgcct tcctgatgcc
     4501 gtagtcgcca aaggggcgtc ggggcgccgc ttccgtggtg ggcacttcgg cggcggggcc
     4561 gtggacccag atcgtgccgc agtgtaggaa atgctgaacg cacccgcgca gggcctcgac
     4621 cagactgcgg acgctctcgg gtgtgaagca gaccatgtcg atcacaacgt cgggctccag
     4681 cgcccggacg cgttcgccaa aagttcccgc gcggtcttcg gcttcccggt ccgccgcaat
     4741 ctgagtgaca tgcgcccatg ccgcatgcag caagtacggg tgctgctgtc cgcggctgat
     4801 ctggatcacc tcgtgaccgg cttcgaccag gcgcggaatc agataggtgc cggtgtggcc
     4861 ggtgcccccg ataacgacga gacgcatgga tgctccttcc tgaacatgac ccgggaatac
     4921 gatggttcgc cgtcagtcag atcttggccg gagagctggc atcaggcggt ggtgagcacg
     4981 atgatggctg cgatgccgca gagcacaccc gcatagccgt aggcgctcag gcgctcgcgc
     5041 aggatcacaa tcccgacgag cgcaacaacg gacagactgc cgccgacggc cacgggaaag
     5101 gccagataac ccggaatgcc ctggcccagc gcgccggtca ggaggaacca gcccgtcgaa
     5161 ctgcacaggc ccatgatgcc cccgatcagg atttcacggg gccgaatgcg ctggcgcaag
     5221 gggagaaagg ttgcaagggc gatcagcagc ccggtgccgt accagaagct caggtaaagg
     5281 taagcgtggg tctccgccag gccatagtcc tgaagtatcc gtgccccgaa ctggctgaac
     5341 ccattggtca gaaatgcaat cagcatccag cgataccaca tgatgtcgca aaccttccgg
     5401 gattaggatc gaccgggggc gcggcccgcg gtcgcctggg atttgacggt ggccggcgcc
     5461 ccaggcgcgg cagccacggc gacaggggcg agcctggcca ggacgacctt atcgcgccac
     5521 agcaggaaca ccgaccccag caccagtaac agcgatacgg gatgctgcca tttgatctcc
     5581 tgcttccact cttgatagat tatcagcgat agtacggccg ggatcagcgt ggaaagattg
     5641 acgatcagcc aactggtggt gatcggacca aagcgcacgc cgatctgaaa ggtcagaatg
     5701 ccgaagctcg cgcaggtgcc gcaaatgatc gcgacaatca ccgcgctgcg cgtaaaggga
     5761 gggaacaggc tttccccctg gcccaagact ttgacgaaaa gcgtatagct ccacatcacc
     5821 acggccgccc agaagaacag ggaaacgttg acggcggagg ggcggcaatc gcgcaaatca
     5881 gccagcttgt gcgtgatgcc caggccagcc agaaccagca gggcgatcac aagcatcaac
     5941 atgccaacgg acatgaattg agtctcactt tctgtcaacg gatccgaccg gcaggcaatc
     6001 cgcgtcttgc cgcgtggctg ccgtcaccag ggccgcgacg tggtgggctg gggtgtattt
     6061 tggcactccg cagccaggac tgaggatcag tcgccccccg ccggcacgcg ccttttccat
     6121 gcaccgggcc gccgccgcgt ggatctcctc aacagggccg ttgagcagga gcaacgtatc
     6181 cacgtttccc cgcagcacga tgcggtttcc gatacgggca aaggccacat ccaggtcgac
     6241 catcgcatcg agttcaaagc aatcggcccc gctcgcttca aggtcatcga gcagatgggt
     6301 cgaattgccg caaacatgca ggaagacctt gacgccctcg gccttcaacg cggcaatcag
     6361 gcgctgatca tagggcaaca ccatctcgcg gtaaagctcg gacgagacca ggctggcgga
     6421 agattcccca aactgaatgg cgtccacgcc gcactgggcc atggcatagc cataggcgat
     6481 cgccacttgc gtgttgaact cgagcaggtc atgaaagcgt tcgggctcgc gcagcgccac
     6541 catgatggcc tgctcagaac cgagcagggt caaagccagc tgaaacggac cgctgtcgat
     6601 attggcctcg acgaaaagcc ggccgtccgc gagctcgacg gccttgcggg cggcctcgag
     6661 catcatgggc atccgcccgt cccgctgcgg atcagggacg cgcaacccgg tcaactcgtc
     6721 gatcgagtcc agcaggtggt gcttgacgcc ccggggttca tcatcctccg gaaggatcag
     6781 cgcgccgccg agggcctcat gcagaatcca gttgtcactg gagatgtaaa tgccatcgag
     6841 acccatctgg tccgagacat caatcacggt ccgcgccagg gtgtgagcat cgccatggaa
     6901 tttcccctgg gggattttcc gcagccaggc ggcgttggca agcgtgctcg ccagcaccgg
     6961 cgtcctgtcg acgggtcgcc cttcaagcag attccggata cgttctctgc cgttcatgat
     7021 cgggacaggg tccttcctgt gcgggatccg gggccgcagc ttcagggata atccagttgc
     7081 acgtcttcga tgccctcgag cggtcccggg ccgtagggtt gcgtcatccc tgcgttgacc
     7141 agcgccagac gccggcgcag ttcctcggcg agatttttcc aacgtcgaat ctggcgtgga
     7201 tcttcgagct cggtcgctag ttccagagcc tcctcgcgcg ctgactccag ggcctcaatc
     7261 gactgcgtct catcccccgc cagttcgtaa aagcgccgag cgagttgttc actgccgtag
     7321 atggccggca agcggttggc gaaccagtca tcccaggaga ggcgcgggtt ccaggtgaag
     7381 tcctcgaaag ccaggtagtt caggtcgttc tgcgggctag ccgcgcccac ctgaccgacg
     7441 atgaccaccc cttcggaggc atcttcgatt gcccgcgcgc aggtctgctg gatgatgtcg
     7501 ggaatgagcg catacgtctg gcccggcgag ccccaccact ggcgcggctg ggaccagtag
     7561 ctgccctgat gcaacaggcc gatggagtgg gtggccccga cggggggctt caggtcggcc
     7621 ggccaacccg ccgggctgcc gatcatgccc gaataagtcc attgaacgat ggcttgctcg
     7681 ggatactgcc gcaggaagga ggggggattc tccatcattt cgcggttgta gccccaatag
     7741 ggactgtagg taaaccagat ggagggattg atgcgcaatc cggtttcaat cacgggcaat
     7801 tgcgtgacca tcatatccca gaggaaattg gggtcgttgc cgggtttggc gcgttcggcc
     7861 tgacagcggt cgcactggca ctcaaaaaag tcgccgtgct cgatgttgac cccgccgata
     7921 tcggggaagg ttgtgaagaa ccactcggag ccctctcgca ggaactgctg caaggccgga
     7981 tcgctggggc aaggggcgtc gatgggttga ccatctttgc cgaccatgcg gcgcacgtcg
     8041 ggatgctctc gcagccaggt ggtcagactc caaggagaat cgccaccgtg atagaaccca
     8101 ccatagatga cggtgccgat gccgggaaga atgcgcacgg cgttacgccg cgcataggag
     8161 gaaaggcgct gggcggactc gacgccgccg tgcgaatcgt tgatgaatcc ccagatgatc
     8221 aggccgttga gcttgtggtc ggcggcataa tccaccacac gctgaaactg attgagatag
     8281 ccgtcgggtt gttccagata gggctcgagg gtgccgttgg ggtcaaccat tctggtctgg
     8341 tgaatgctgg ggatatcctc gttccaattc gtcatgaagt tccagttcca cagaatccgc
     8401 ttggagaaca tcggcgagcg ctcgacatgg agattggctg gaaccgtgat gccctcgcgg
     8461 tcgatttgcg cgtgatagtt ccagaattcg cctacgccat agcgcagccc gctatcgtcg
     8521 gcgccgatga tccagatgcc cggcgaatcg ccccgcgtgg caatcacgaa cccctcggca
     8581 tcaagccggg catccttgac tagagaaggg acgtcagttg cgaacgaatt gagatcggcc
     8641 ttcgtgccga ggtagatcgg caacttgtca accggcaggg tcgagccggt cgtcaggagg
     8701 tcgaagctta cgttggcgtc gcgctggcca agggctttca gccgttcggc cggaaccttg
     8761 gccgcttcgg cggctacgat cacggcgcga tcggccaggg caaattgatc actgcgagca
     8821 ttggcgatcg tcagggtgtc aactgcaagg gccggtgctg ccatcagcag catcagggct
     8881 cccaatatga gcgttcgtag agcgaaaccc gtgtagcgaa tcatcatgca tccttactag
     8941 ccgaaagtga agaattcatc gccgccctca tgaggaaaga gttcccatgc tccacctggt
     9001 taaagtcaga cgtgatcctg tccgagcgca cgctcaaggt ctggaaaccc ctgtgataaa
     9061 accatgtaac acggccgagg ggggaaaggt caaggggaat cggtcacgga tttttccggt
     9121 tgcctgattc tgataaaagc ccgcaccagc cgactttgaa agcaatcgga ggtgatccat
     9181 tgaccacttg gaagacgatg gacgcttgtt gattgtctgt gattggccgg tggatcgcag
     9241 gttacgcccc gacagaatcc atgggcaagg ggaggggtcg ggcgtgccaa tagcgcgtgc
     9301 gaagagcctc tcttcttttg cttgttttct taggttaaat gcttttacta tggcggcctc
     9361 gatactctac acaggacgca cgcgggaaaa cctgaaagga cgcttcgagc gatgaatcgg
     9421 accctgaact gttggctggt atttgccctg gcgctagtga tccgtcccct tggcgcggag
     9481 caattgcccg cttgggatgc tccccagttc atgaccctga tcgaacaggg acgcccccaa
     9541 ggcgcgatca tcctggcgga tcaggccacg gccaccgagg aaattgccgc gcgcaaactg
     9601 gccgagtggt tgggcgagca gagtggtgtc gaatttccca ttttgcggac ccgggagatg
     9661 gatgcgcact ccacagggaa ttttgtcctg ctggggactc cccaaaacca accgctgctg
     9721 gccgaactgg tgatcgaagg cagtggcggc gcggccgatc tgccattcct tgatgacgaa
     9781 gggttcggcg tggagacctt cagccgggat ggccgaaatt acctgctggt cggcggacgc
     9841 actccggcgg gagtttttca tggcgcgatc tatgcgcgcg acttcctgtt tgacattctt
     9901 caccctgtcg atcagatcgt ggtgcgcgat gtcgagctgg tgcgaaaccc ggctctgctt
     9961 gagcgcggcc cgtacctgtt gccgcagtac ggagtggtgc cggaatacac gctggagcac
    10021 tggaagcata tcatcgatcg catggcggag gggggcctca accgcattta ctggtgggtg
    10081 gcggggatgt atacgtcccc gcgatttccc gaaacattca ccatcaccaa tagcaaaatg
    10141 ggtgttgagg atatcaacac actgatcgat tatgcccacg agcgcgggat gaaattcctc
    10201 gtcggtggcg gcgccttctt ctggcatggc gtcgatggct atctggagga tcatcccgag
    10261 ctggccgcga cctcgtcggc gggcatgtgt ccttccaacc cggaagcgcg tcgattcatg
    10321 caggcttact cgctggaatg gctggaaact tttcccaagg ccgacggatt gtggctggag
    10381 ccccgcgacg aagcgggcgc gtgcttctgc gaggattgcc aggtccggct ggatgcgttc
    10441 aattcgcgcg cgtatgggca gaatgaaatc agctttctca aggatctgat gaagcaggta
    10501 tgggatcgca atcccgcgca taccctggtc tggttgattg aattccttga gcacaaggac
    10561 tttttcccgc atctggacga cccgctgtat ttcgaaagga ttcgcgaaat ccaggattcc
    10621 cgcgtcaaat ggatggtcgt ttgggaagcc ttcaacctgc cggggccggg caatgaccga
    10681 cgaccggttc cctttttcac gcgcaatggg ttgcactggg acaaacccta ttggcctaac
    10741 ctgcaaaatg tgttcgacca tgcgcgcatc tgcgctgagc aagggtatct gggctattcc
    10801 aacgcctggg aaatcggctt tgccagcaac gactggtata tccatgatgt gccttatccc
    10861 gttgatatca ttcccgaggt ggtaacccgg ttggcattcc gcgaggcctg ctgggctccg
    10921 gaacaatcct gggatgcttt tacagagcgt gttcatcggt atttcttcag tcgcgaggct
    10981 ccgcgccaat tggccgagga catcctctgg ctgaggcagt atatcaccgc ggccaatcat
    11041 gaatttcggt cgccgatgga attcgacacc gaaacggctc tggcggatga agtcaggcgc
    11101 gtcgatcaac ttgaagatcg gactgaaaag gcgtccgccc tggaacggct ttccgccctg
    11161 gtcgatattc tggaggagac ccgcgcggag gccttgcccc ggatgttaga aatcgaggcg
    11221 cggctggcgg agatcgaacc gcaagcctcg cgcaagaccc tgcagacgct cgccatcatg
    11281 cggcgtgcga ttgatgatag tcgggctgtc tatgcgacag ccgttcccga tgatgcaacg
    11341 ctcgacgccg ccctggccgc cgtggccagg atgaaagagg gggcaagaga agaggatgcc
    11401 cgcagggacg cggcgaccga cgtgtacctc acggaacatg gccgcgccgt tgcccacgtc
    11461 tatgctccgt tcgacaatga atgggcgggg cgccgcctgg ccggtcgact caaacagtgg
    11521 acgggaagcg atctgaccct gcagtgcgca ggggccaagc ctgaaggatc tggcgacctg
    11581 attgccattg gcaccgtgga atccaatccc gtagtgcgtg aggtcctggg cgagaatccc
    11641 cgcctggagg ggctcggcga agaaggctat attctgcaga ccatacgctg gaaggatcgt
    11701 c
//
LOCUS       DAOPCO010000113        11626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5457_length_11626_cov_4.787400, whole genome shotgun sequence.
ACCESSION   DAOPCO010000113 DAOPCO010000000
VERSION     DAOPCO010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11626
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5457_length_11626_cov_4.787400"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..176
                     /gene="yidD"
                     /locus_tag="PLS90_09290"
     CDS             9..176
                     /gene="yidD"
                     /locus_tag="PLS90_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPK02638.1"
                     /translation="MLRRLVRCQPFHPGGYDPVPGADPDEDRRRAALRPRGRRRPFAH
                     DHPAAHPHSGS"
     gene            191..2074
                     /locus_tag="PLS90_09295"
     CDS             191..2074
                     /locus_tag="PLS90_09295"
                     /inference="COORDINATES: protein motif:HMM:NF026199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HPK02639.1"
                     /translation="MDKSQVQRLLLFVALSLMIMQLTSWWTRRNAPPAPAPAELREGV
                     ARSDRAATTTLTQALQPAPQATPAPAEPRPAADELTLTTPQFEVVFSTAGAVPVRWDI
                     IDPEYVTPRSPDDLAAINRDAPLVPEGHEPLIDPRLAHQGDYPLPFELVLREMGQSYL
                     NELNSDVYTHEPVREEGFTGYRFTSPLVEPGVRVAKTFLLAEQGFAGRLRIQVTNESQ
                     FSLTFDSGTGGLGVTLGPGLGLPFDDDNPIARFASVSAVAQTEQEFFYYKMKEPGESQ
                     ALNTPSLRWGGVASMYYLISLIPDGQTPVTTFKSMIPSELARFIPPQRQKEHLPYYPA
                     AEIYTPPFDLGPGQQFTAGFDLFAGPRQHKVLEQAGHDLDRVMFYDSTRVMRGLCLIL
                     MRLLDWFHGLVANWGMAIILLTVFVKLVTFPLVHKGLKSQAKMTAEMNRIKPLIDKLN
                     EKYKDNPQKKQQEMFKLYREHGVNPLGMFKGCIWMAIQIPIFYALYRLLYQSIDLRGA
                     SFLWVADLSRPDMLFRLPVALPFIGEWFNLLPLITAASQMAMSKFMTTPPTDPQQAQM
                     QKTMMYFMPVIILFITYRFPSGLMLYWLVSNLWQVLQQVWVNKHIRKPPQASAHAPAR
                     ASS"
     gene            2133..2753
                     /locus_tag="PLS90_09300"
     CDS             2133..2753
                     /locus_tag="PLS90_09300"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPK02640.1"
                     /translation="MSDIVVAGNDEAQMLEEALQRLGLPREAVDYHVTREKEEDLLPG
                     ATPLMQMHVSVKSEYVADKALDHLEAILSILDIEAELEDEIRNGIIFIHITSKQAASL
                     LIGRDGQNLDALQHLITRMVMRVGRDSPLVVIDIEGYRMRQFAKLEKLGQLAMKRARE
                     TGNEIELDPMPPLERKYLHHYLKSEPGITTFSRGEEPERYLVIIAD"
     gene            2843..3592
                     /gene="hisA"
                     /locus_tag="PLS90_09305"
     CDS             2843..3592
                     /gene="hisA"
                     /locus_tag="PLS90_09305"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPK02641.1"
                     /translation="MFLPIPAIDLMNGQVVRLSRGRAQEKTVYSDDPVAVARQFEALG
                     ARRLHVVDLDGAFTGQPRNLEILRRIREATALQIEMGGGLRTRDLVERVLGLGLDFAI
                     LGTSALRDRALIAGLAADYGDKLIVGIDAKDGRVAIEGWVETSELTAIDFARELQAMG
                     VGTVIYTDIATDGMLSGPNLSAQAEMADAVAMRVIASGGIRDVADLRALRDLNRPNLI
                     GAITGRAVYEQRLSIAEAVQAMAEANGASRP"
     gene            complement(3504..4931)
                     /locus_tag="PLS90_09310"
     CDS             complement(3504..4931)
                     /locus_tag="PLS90_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02642.1"
                     /translation="MLVATICWTRGLRFADPPEGMWDFFWQAIPKADLIARPWQSIWR
                     LHAQPPGYQLWGLFWLALGGPERFPANVQIGHVLLGGLVTAFSWKLAHALTGSRAWAT
                     LAGLLTALNPSLFLYEGYLIYEMLVIALVTGAGVALWRTLREGRTRWLVLYCALLNLL
                     VLTRSLYHLVFLAAALAPAWPALRRLRPWGRVLLLAGTFALPVAWYGKNYCQYGFFGA
                     SSWFGMGLFKTVSTYYLQPDWEAVEPAIRPDYPYVLDKYAYQHAIAEYEAYGFDRESE
                     VPLLSRNNLHNINVPEISAGYGAAALKLIRLRPALYLLAVLDGYGSFCRTPGTFVHLS
                     VNWEQVPWLPVYREAIYGEWLESEFSVHLPFSIGSMMFFYFPALMLGGCATVGYWRRR
                     ARARGLFDPRPWLLGWMVLVCVYVAIIGSLFENGENMRFRFATEPLTFAIGLLLIRGG
                     WRRWLRPWPGRLRQSRGAARRRPGR"
     gene            5059..5490
                     /locus_tag="PLS90_09315"
     CDS             5059..5490
                     /locus_tag="PLS90_09315"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPK02643.1"
                     /translation="MERRHQERVRILKLAIDKRQRLEPVYLFRPKDEYADRAAISALI
                     YDISRTGMRIWFHSDDLFEDEQFTVVVYPYLSLSLNKFEVPVRRVWINTRDPACTELG
                     VEYHELTAQQEARIESLMRNFRGHSEEVFLRCLLLPSSLPR"
     gene            complement(5506..7137)
                     /locus_tag="PLS90_09320"
     CDS             complement(5506..7137)
                     /locus_tag="PLS90_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02644.1"
                     /translation="MVDTLPPASAEVPRVLIIDDEFDVRQITSIALQYEGWMVREASN
                     GREGLAIAREYRPHLILVDLMMPEMTGVEFCRRLTEIPDLRDVSILLISAVSERAKVL
                     NDFWELPLARKNYLHKPFTSDQLVECVKGLVPRFQTGSAPIRPEPAPRPRPAAAPTPP
                     AMPARSAPSEPKKPVMAASPSQAGGLGDHRAGYRVLIVDDDPDIRMILSTALGIYHTV
                     ETAENGKLGLAALDRFLPDFIIMDVNMPVMKGPETAEAIRKHPRLRHVPIFFLTAETD
                     KDLPRKLYDVGGNLYLKKPLDPQQLLKFIDFFLKETELEREGNYKKFKDAPPIEQPTA
                     PAAGAPAAPTRAPARAAAPTGGTSVRILTIDASREEQVLLRQLIQGSDGQPPLVSGGP
                     YELIWSDQPRPALANLGDWEPDVILYNPRNPGLDGVAFGQTLKLEHQLDPCQVAFVGT
                     RFYDADHAYSERHFGRKVIDLSMDRQAIARGLEGVLAEAARNPRPKKLSFEALQRQDR
                     DDLRQQAAQQDRLTKERESFRRRYAGIQQFIDRNF"
     gene            complement(7331..8842)
                     /gene="guaB"
                     /locus_tag="PLS90_09325"
     CDS             complement(7331..8842)
                     /gene="guaB"
                     /locus_tag="PLS90_09325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPK02645.1"
                     /translation="MANAHLDEFMEHFRHTALTYDDVTLLTQYADFLPADTDLRTRLT
                     RNIELNVPFVSAAMDTVTEAEMAIAMALQGGIGIIHKNLKPTTQRSNIKRVKYYLNGF
                     LTKARTMEPDQTVADVMRIKAEKNWSFHSFPILDDNRRLLGIITGRELKYCNDPKTPI
                     RELMISQPVTGPEGTTIEEAYATLCRHRISILPIVKPDGTFLGMYCFKDVKEILTGSH
                     PLYNRDEQHKLRCGAAVGPNTYERVEILMESPVDVLVVDTAHGYSAGVIEMIRWIKRH
                     YPRVDVIGGNVATAEGARALIDAGADGVKVGVGPGSICTTRVVAGVGVPQLTAVYECA
                     RAARGTGVPIIADGGIRYSGDVAKALAAGASSVMMGSVLAGTDEGPGERVLYHGRQYV
                     IYRGMGSIEAMAQRYGSADRYGQAQTTPEKLVAEGIEGMVPYAGSVNDVLAQFIGGLR
                     NALGYNGCRTIPELQERARMVRVSDAGRRESHPHDITLTKDAPNYKVMGGDGL"
     gene            complement(9114..9713)
                     /locus_tag="PLS90_09330"
     CDS             complement(9114..9713)
                     /locus_tag="PLS90_09330"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018937146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPK02646.1"
                     /translation="MLLLIDNYDSFTYNLAQYLGELGHEPAVHRNDQLTVARIEAMAP
                     AGIIISPGPCTPNEAGISLEVVQRLGARIPILGVCLGHQAIGQAYGGRVVRAPEIMHG
                     KTSQIFHNGAGVMAGLPNPFEATRYHSLIVEEATLPDCLEVTAWTEEPHRLIMGLRHR
                     RHPVQGVQFHPESILTAVGKDLLRGFLQTLKLETPQSAM"
     gene            complement(9797..10285)
                     /locus_tag="PLS90_09335"
     CDS             complement(9797..10285)
                     /locus_tag="PLS90_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02647.1"
                     /translation="MFANTHDIHSQIRQNAEQLNQIHEKMHYNDYDSHRFFSKFDELA
                     WPGGLNEGKRRLREGDPEEIEIAICFLEEDPSFYRSSNIKSDVIRILCRHPLSEAQKK
                     RLGDAICRLVDTRLGREFIALGRLAKKIDNPRFRGQLYQRLNSDSLEIRQRAQTLVRA
                     MQ"
     gene            complement(10407..11234)
                     /gene="murI"
                     /locus_tag="PLS90_09340"
     CDS             complement(10407..11234)
                     /gene="murI"
                     /locus_tag="PLS90_09340"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPK02648.1"
                     /translation="MRLGVFDSGIGGLTVLRAIRRRWPGHATVYLGDTARVPYGIKSR
                     QTVIHYARQNTRLLLAEAVDLLVIACNTASAFALEALRDLPVPVVGVIEPGAAAAVGA
                     TRSGRIGVIGTAATIASGAYEAAIRRLAPAARVSSRACPLLVPLAEEGWETTAVARAT
                     LERYLEGWLPAPASDPETPDVLVLGCTHYPVFKPLLSELLGPGITLIDSADAVAAALA
                     PWLDAAPPEPAPGHRLLVTDAADGFARAARRLLGETVIDLQVVDLLPAVADKQLQEG"
     gene            complement(11231..>11626)
                     /locus_tag="PLS90_09345"
     CDS             complement(11231..>11626)
                     /locus_tag="PLS90_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018865936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPK02649.1"
                     /translation="CAETVGAETAEKLRGYSLRLYRYAHEYLAERGIVLADTKFEFGW
                     VDDEIILIDELLTPDSSRFWVKGSFEAGGEPISFDKQYVRDYLETLDWNKQPPPPPLP
                     AQVVANTAARYREIFEKITGQAIDGAEVP"
BASE COUNT         1991 a         4034 c         3626 g         1975 t
ORIGIN      
        1 tcgcgctgat tctccgccgc ctggtgcgct gccagccgtt tcatcccggc ggctacgatc
       61 cggttcccgg cgcggatccc gatgaggacc gccgccgcgc ggcgctccgc ccccgcggcc
      121 ggcgccggcc gttcgcgcac gaccaccccg ctgcccatcc ccattcagga tcctgaagtc
      181 aaccggcccc atggataaat cgcaggttca acgtctcctt ctgttcgtgg ccctcagcct
      241 gatgatcatg caactgacct cgtggtggac gcggcgcaac gcgccgccgg cccccgcgcc
      301 ggccgaactg cgcgagggcg tcgcccgctc cgaccgcgcc gccacaacga ctctgaccca
      361 ggcgctccag cccgctcccc aggccacccc cgcgccggcc gaaccgcgcc ccgccgccga
      421 cgaactcacc ctgaccaccc ctcaattcga agtcgtcttt tcgaccgccg gcgccgtccc
      481 ggtccgctgg gacatcatcg accccgagta tgtcacgccg cgttcgcccg atgatctcgc
      541 cgccatcaat cgcgacgccc cgctcgttcc cgaggggcat gagcccctga tcgatccccg
      601 cctggcgcac cagggcgact acccgctccc tttcgagctg gtcctgcgcg agatgggcca
      661 gagctacctc aacgagctca acagcgacgt ctacacccat gaaccggtgc gcgaggaggg
      721 cttcaccggc taccggttca cctcgccgct ggtcgagccc ggcgtgcgcg tcgccaagac
      781 cttcctgctg gccgagcagg ggtttgccgg ccgcctgcgc atccaggtca ccaatgagtc
      841 gcagttcagc ctgacgttcg acagcggcac cggcggcctg ggcgtcacgc tgggccccgg
      901 tctggggctg cccttcgacg acgacaatcc gattgcccgc tttgcttcgg tcagcgccgt
      961 cgcccagacc gagcaggagt tcttttatta caagatgaag gagccgggcg agagccaggc
     1021 gctcaacacg cccagcctgc gctggggcgg cgtcgcctcg atgtattacc tgatctcgct
     1081 cattcccgac ggccagaccc ccgtcaccac gttcaaaagc atgatcccct ccgaactggc
     1141 gcgcttcatc cccccccagc gccagaagga gcacctgccg tactaccccg ccgccgagat
     1201 ctacacgccg ccgtttgacc tggggcccgg ccagcagttc accgccgggt tcgacctgtt
     1261 cgccggcccg cgccagcaca aggtcctcga gcaggccggc cacgacctgg accgcgtcat
     1321 gttctacgac tcgacccgcg tcatgcgcgg cctctgcctg atcctgatgc gcctgctgga
     1381 ctggttccac gggctggtgg ccaactgggg gatggcgatc atcctgctga cggtctttgt
     1441 caagctggtt accttcccgc tggtccacaa ggggctcaag tcgcaggcca aaatgacggc
     1501 cgaaatgaac cggatcaagc cgctgatcga caaattgaac gaaaaataca aggacaaccc
     1561 ccagaaaaag cagcaggaga tgttcaagct gtatcgcgag catggggtca acccgctggg
     1621 gatgttcaag ggctgcatct ggatggcgat ccagatcccg atcttctacg ccctctaccg
     1681 gctcctgtat cagtcgatcg acttgcgcgg ggccagtttc ctgtgggtgg ccgacctgtc
     1741 gcggcccgac atgctgttcc ggctgcccgt cgcgttgccg ttcatcggcg agtggttcaa
     1801 cctgctgccg ctgatcaccg ccgcctcgca gatggcgatg agcaagttca tgacgacccc
     1861 gccgaccgac ccgcagcagg ctcagatgca gaagacgatg atgtacttca tgccggtcat
     1921 catcctgttc ataacctatc gctttccctc ggggctgatg ttatattggc tggtttccaa
     1981 cctgtggcag gttctgcagc aggtgtgggt caacaagcac atccgcaaac cgccgcaggc
     2041 gtccgcccat gcgccggcgc gggcgtcgag ttgaggggaa ccctcgccgc cgcgctgccg
     2101 gagggagctt cgccaggaag gaatcgacat cgatgagcga tattgtcgtg gccggcaacg
     2161 acgaggccca gatgctggag gaagcgctgc aacggctcgg gctgccgcgc gaggccgtcg
     2221 actaccacgt cacccgcgag aaagaggagg atctgctgcc gggcgccacc ccgctgatgc
     2281 agatgcacgt gtcggtcaag agcgagtatg tcgccgacaa ggcgctcgat cacctcgagg
     2341 cgattctctc gatcctcgac atcgaggccg aactcgagga cgagatccgc aacggcatca
     2401 tcttcatcca catcacctcc aagcaggccg cgtcgctgct gatcggccgc gacggccaga
     2461 acctggatgc cctccagcac ctgatcaccc gcatggtgat gcgcgtcggc cgcgactcgc
     2521 cgctggtcgt gatcgacatc gagggctatc gcatgcgcca gttcgccaag ctggaaaagc
     2581 tgggccagct cgcgatgaag cgcgcgcgcg aaaccggcaa cgagatcgag ctggatccca
     2641 tgccccccct cgagcgcaaa tacctgcacc actacctgaa gagcgaaccg ggcatcacca
     2701 ccttcagccg cggcgaggaa cccgagcgct acctcgtgat catcgcggat tgagcaatcg
     2761 gcaattcaac gttccagatt cgccatggct tgaatgatga acctcgagct ttgaactttg
     2821 attcctgact attgaatccc tgatgttcct cccgatcccc gccatcgatc tgatgaacgg
     2881 ccaggttgtg cgactcagcc ggggccgggc gcaggaaaag acggtttact cggacgatcc
     2941 ggtcgccgtc gcccgccagt tcgaagcgct cggggcgcgg cggctgcacg tcgtcgatct
     3001 cgacggcgcc ttcaccggcc agccgcgcaa cctggagatc ctccgccgca tccgcgaggc
     3061 gacggcgctg cagatcgaga tgggcggcgg cctgcgcacg cgcgacctcg tcgagcgcgt
     3121 cctcggtctc gggctcgatt tcgccatcct cggcaccagc gccctgcgcg atcgcgccct
     3181 catcgccggg ctcgccgccg actacggcga taagctgatc gtcggcatcg acgccaagga
     3241 tggccgcgtc gccatcgaag gctgggtgga aacgtccgag ctgaccgcca tcgacttcgc
     3301 ccgcgagctc caggcgatgg gcgtcggcac cgtcatttat accgacatcg ccaccgacgg
     3361 catgctcagc gggccgaacc tgtcggcgca ggccgagatg gccgacgccg tcgccatgcg
     3421 cgtcatcgcc tctggcggca tccgcgacgt ggccgatctg cgcgcccttc gcgatctgaa
     3481 ccggcccaat ctgatcggcg ccatcaccgg ccgggccgtc tacgagcagc gcctctcgat
     3541 tgccgaagcc gtccaggcca tggccgaagc caacggcgcc agccgcccct gatcagcagc
     3601 agtccgatcg caaacgtcag cggctccgtc gcgaagcgga accgcatgtt ctcgccgttc
     3661 tcgaacagcg agccgatgat cgccacgtaa acgcagacca gcaccatcca acccagcagc
     3721 cacgggcgcg ggtcgaacaa cccccgcgcg cgcgcccgcc gccgccagta cccgaccgtc
     3781 gcgcagccgc cgagcatcag cgccggaaag tagaagaaca tcatcgaccc gatcgaaaag
     3841 ggcagatgga cgctgaattc cgactccagc cattctccat agatcgcttc gcgatagacc
     3901 gggagccagg ggacctgctc ccaattgacg ctcagatgga caaacgtccc cggcgtgcgg
     3961 cagaagctgc cgtagccgtc cagcaccgcc agcaggtaga gcgccggtcg cagccggatc
     4021 agtttgagcg ccgccgcgcc gtagccggct gagatctccg gaacgttgat gttgtgcaga
     4081 ttgttgcgcg agagcaacgg aacttcgctc tcgcggtcga agccgtaggc ttcatactcc
     4141 gcgatcgcgt gctggtaggc gtatttgtcc agaacgtagg gatagtccgg tcggatcgcc
     4201 ggttccaccg cctcccagtc gggctggagg taatacgtgc tgacggtctt gaacaggccc
     4261 atgccgaacc agctcgacgc gccgaaaaag ccgtattggc aatagttttt gccataccac
     4321 gccaccggca acgcgaacgt cccggccagc agcagcaccc ggccccaggg ccgcagccgc
     4381 cgcagcgccg gccaggccgg cgcgagcgcc gccgccagga acacgaggtg atacagactg
     4441 cgggtcagga ccagcagatt gagcagtgcg cagtaaagca ccagccagcg cgtgcgcccc
     4501 tcccgcagcg ttcgccacag cgccaccccc gcgccggtga cgagcgcgat taccagcatc
     4561 tcgtagatca gatacccctc atacagaaaa agcgacgggt tgagcgctgt cagcaggccg
     4621 gccagcgtgg cccacgctcg gctgccggtc agcgcgtgcg ccagtttcca gctgaacgcc
     4681 gtgacgaggc cccccagcag cacatggccg atctgcacgt tggcggggaa gcgctcgggc
     4741 ccgccgagcg cgagccagaa cagcccccag agctgatagc cggggggttg cgcatgcagg
     4801 cgccagatgc tttgccaggg gcgggcgatc aggtcggcct tggggatcgc ctgccagaag
     4861 aaatcccaca tcccctcggg gggatcggcg aacctcagtc cccgggtcca gcagatcgtt
     4921 gcgacgagca catgcagcgc cgccggagcc cagcatgcga ccgcctccgc tcgggcgatt
     4981 ccgccgattg tcggagcgtt tgcgctgtga tacggtcgtg agtcggaatc ggatgcggga
     5041 gaccgatgga tgggaaccat ggaacgacgt caccaggaac gggtccgcat tctgaagctg
     5101 gcgatcgata agcgccagcg cctcgaaccg gtctatctgt tccgacccaa ggacgaatac
     5161 gccgaccgcg cggccatcag cgcattgatc tatgatatca gtcgcaccgg catgcgcatc
     5221 tggtttcact ccgacgacct gttcgaggat gagcagttca ccgtcgtcgt ctacccctac
     5281 ctgagcctct ccttgaacaa gttcgaagtg ccggtgcgcc gcgtctggat caacacccgc
     5341 gacccggcct gcaccgagct gggggttgaa taccacgagc tgaccgcgca gcaggaagcg
     5401 cgcatcgaga gcctgatgcg caacttccgc ggccacagcg aggaagtctt cctgcgctgc
     5461 ctgctgctgc cgtcgagtct tccgcgctga gcggcggcga tcggctcaga aatttcggtc
     5521 gatgaactgc tggatgcctg cgtagcggcg gcggaacgat tcacgttcct tcgtcagccg
     5581 gtcctgctgc gccgcctgct gccgcaggtc gtcgcgatcc tgacgctgaa gcgcttcgaa
     5641 actcaatttc ttcggccgcg ggttccgcgc cgcctcggcc agcaccccct ccagcccgcg
     5701 cgcgatcgcc tgccgatcca tcgacaaatc gatcaccttc cggccaaagt gccgctcgct
     5761 gtaggcatga tcggcgtcat agaaccgcgt cccgacaaag gccacctggc acgggtcgag
     5821 ttgatgctcc agcttgagcg tctggccgaa ggcaacccca tcgagcccgg gattgcgtgg
     5881 attgtagagg atcacgtccg gctcccagtc gcccaggttg gccagcgcgg gccgcggttg
     5941 gtcgctccag atcaattcat acgggccgcc gctcaccagc gggggctgcc catcggagcc
     6001 ctgaatgagc tggcgcagaa gcacctgttc ctcgcggctg gcgtcgatcg tcagaatgcg
     6061 cacgctggtc ccgccggtgg gcgcggcggc ccgggcgggg gcgcgcgtgg gcgcggccgg
     6121 cgctcccgcc gcgggcgcgg tgggctgttc aatcggcggc gcgtccttga atttcttgta
     6181 attcccttcc cgctccagct ctgtctcctt gaggaaaaaa tcgatgaact tgagcaactg
     6241 ttgcggatcg agcggcttct ttaaatagag attccccccg acgtcataca gcttgcgcgg
     6301 cagatccttg tcggtctccg ccgtcaggaa aaagatcggc acgtgacgca gccgcgggtg
     6361 tttgcggatg gcttcggccg tctccggccc cttcatcacc ggcatgttca cgtccatgat
     6421 gatgaagtcg ggcagaaacc ggtccagcgc cgcgagcccc agcttgccgt tctcggccgt
     6481 ctcgaccgta tggtagattc ccagcgccgt gctcaggatc atgcggatgt cggggtcgtc
     6541 gtcgacgatc agcacccggt agcccgcacg gtgatcgccc agcccgccgg cctggctcgg
     6601 cgatgcggcc atcacgggtt tctttggttc ggagggcgcc gacctggcgg gcatggccgg
     6661 cggcgtcggc gcggcggccg gccgcggtcg cggcgccggt tccggccgaa tcggcgccga
     6721 tcccgtctgg aaccgcggca caagcccctt gacgcactcc acgagctggt cgctggtgaa
     6781 cggcttgtgc aggtagttct tgcgcgccag cggcaactcc cagaaatcgt tcagcacctt
     6841 ggctcgttcg ctgacggcgc tgatcagcag gatggagacg tcgcgcagat cggggatctc
     6901 cgtcagccgg cggcagaatt ccacccccgt catctcgggc atcatcaggt cgaccaggat
     6961 cagatgcggg cggtattccc gggcgatggc gagcccttcg cgcccattgc tggcctcccg
     7021 caccatccag ccctcgtatt gcagcgcgat gctggtgatc tggcgcacgt cgaattcatc
     7081 atcgatgatc aaaacgcgcg ggacttccgc ggaggcgggc gggagggtgt ctaccatggc
     7141 gggatgcgat gcttccaggg gcccatgccg cgggcgaggg cagcgggccg gattcctcta
     7201 cgccggatgg cgtctgtgcc ttcgatcata ggtcggccgc ggggccggtc aagaacggaa
     7261 aaaccccgcc cctcgtccct cgtcctcgct cccgaaggct cacccaaaac tcggcactcg
     7321 agaatcggcc ctacagcccg tccccgccca tcaccttgta attgggcgcg tccttggtca
     7381 gcgtgatgtc gtgggggtgc gattcgcgcc ggccggcgtc ggagacgcgc accatccggg
     7441 cccgctcctg caactccgga atcgtccggc agccgttata gcccagcgcg ttgcgcagcc
     7501 cgccgatgaa ctgcgccagc acatcgttca ccgaaccggc gtagggcacc atcccctcga
     7561 tcccctcggc caccagtttc tccggcgtcg tctgcgcctg gccgtagcgg tcggcgctgc
     7621 cgtagcgctg cgccatcgcc tcgatcgagc ccatcccccg gtaaatcaca tactggcggc
     7681 cgtggtagag cacgcgctcg cccggcccct cgtcggttcc cgccagcacg ctgcccatca
     7741 tcacgctgga ggcccccgcc gccagcgcct tggccacatc gccgctgtag cggattcccc
     7801 cgtcggcgat gatcggcacg ccggtgccgc gcgccgcccg ggcgcattca tagaccgccg
     7861 tcaattgcgg cacgccgacc cccgccacga cgcgcgtcgt gcagatcgaa ccgggaccga
     7921 cgccgacctt cacgccgtcg gcccccgcgt cgatcagcgc ccgcgccccc tcggccgtcg
     7981 ccacattgcc cccgatcacg tccacccgcg ggtaatgccg cttgatccag cggatcattt
     8041 cgatcacgcc cgccgaatag ccgtgcgccg tatccacgac cagcacatcg accggcgatt
     8101 ccatcaggat ctcgacccgt tcatatgtat tggggccgac cgctgcgccg caacgcagct
     8161 tgtgctgctc gtcgcgattg tagagtggat ggctccccgt caggatctcc ttgacgtcct
     8221 tgaagcagta catcccgagg aacgtcccgt ctggcttgac gatcggcagg atcgagatgc
     8281 gatggcggca cagcgtggcg taggcctcct cgatcgtcgt cccctccggc ccggtcaccg
     8341 gctggctgat catcagttcg cggatcggcg tcttcggatc gttgcagtat ttcagctcgc
     8401 ggccggtgat gatccccagc aggcggcggt tgtcgtccag aatcgggaag gaatgaaacg
     8461 accagttctt ctcggccttg atccgcatca cgtccgcgac cgtctggtcg ggttccatcg
     8521 tgcgcgcctt ggttagaaaa ccattaagat aatatttgac tcgcttgata ttcgaacgct
     8581 gcgtcgtcgg cttcaggttt ttgtggatga tcccgatccc cccttgcagc gccatcgcga
     8641 tcgccatctc cgcctcggtc acggtgtcca tcgccgcgct gacgaacgga acgttcagtt
     8701 cgatgttgcg cgtcaggcgg gtccgcagat cggtgtcggc cggcagaaaa tcggcgtatt
     8761 gcgtcagcag cgtcacgtcg tcgtacgtca gcgccgtatg ccggaaatgc tccatgaatt
     8821 catccaggtg ggcattggcc atcgagaaca accacctccc gcaacgctcg cgccgaacat
     8881 cggacccgcc gccccacccc tgggtacagc ggctaatttc cctagtgaaa caggaacacg
     8941 gaagcttgtc aacgagcccc cagcctgcac gtgaaaagcg cccgttcagc tcgcgccgat
     9001 tcgtgcgact ggaacagcat gctgcaggtt ggctcggccg cgggcgccat cttgccggca
     9061 caattgcgga cggcggcaag acgctgcgca acctaatcca cagttcgccg cgatcacatc
     9121 gctgattgcg gggtttcgag cttgagcgtc tgcaggaacc cgcgcagcag gtccttgccg
     9181 accgctgtga ggatcgattc gggatggaac tggaccccct ggacgggatg ccgacggtgg
     9241 cgcagcccca tgatcagccg gtggggctcc tcggtccagg cggtgacttc caggcagtcg
     9301 ggcagcgtcg cttcctccac gatcagcgaa tgatagcgcg tcgcctcgaa cgggttgggc
     9361 aggccggcca tgacccccgc gccgttgtgg aagatttgcg aggtcttgcc gtgcatgatt
     9421 tccggcgcgc gcacgacgcg gccgccgtag gcctgcccga tcgcctgatg ccccaggcaa
     9481 accccgagga tcgggattcg ggcgccgagg cgttggacga cttccaatga aatgcccgcc
     9541 tcgttgggcg tgcacggccc cggcgagatg atgatcccgg ccggcgccat cgcctcgata
     9601 cgcgccaccg tcaactggtc gttgcggtgg acggccggct cgtggcccag ttcgcccagg
     9661 tactgcgcca ggttgtaggt gaaggaatcg tagttatcaa tcaggaggag catcggctgc
     9721 gttgcggatg gaaatcaggc cgctcccccg cgcgccgggt gacgccgcgc ggcgagccat
     9781 ccctgatcgt atcacatcac tgcatcgcgc ggacaagggt ttgtgcccgc tgccggatct
     9841 ccagcgagtc ggagttgagc cgttggtaca gttggccgcg gaagcgggga ttgtcgatct
     9901 ttttggccag ccggcccagg gcgatgaatt cccgtcccag ccgggtgtcg accagccggc
     9961 agatcgcgtc gcccagccgc ttcttttgcg cctcgctcag cggatggcgg caaaggatgc
    10021 ggatgacgtc ggacttgatg ttgctcgagc gatagaacga gggatcttct tcgaggaaac
    10081 agattgcaat ctcaatctcc tcggggtcgc cctcgcgcag gcgccgtttg ccctcgttca
    10141 ggccgccggg ccaggcaagc tcatcgaact tcgagaagaa ccgatgcgaa tcatagtcat
    10201 tgtagtgcat cttctcgtgg atttgattga gttgctccgc gttctgacgg atctgcgagt
    10261 ggatatcatg cgtgttggca aacatgacgc acccatcggc ccatgggatc gggtggcggc
    10321 ggcaaaccgc ctgggcgggg cccaacggca tccggcaagg aaccgcccgt gcaaggcgtg
    10381 ccttcctcgt cgcgacgacc gtcgactcag ccttcctgaa gttgcttatc ggcaacggcc
    10441 gggaggaggt caaccacctg cagatcaatt accgtttcgc cgagcaggcg gcgcgcggcg
    10501 cgggcgaacc cgtcggcggc gtcggtcacc agcaggcgat gtccgggggc cggctcgggc
    10561 ggcgccgcgt cgagccaggg ggccagcgcc gccgccacgg catccgccga atcgatcagc
    10621 gtgatccccg ggccgagcag ttcgctcagc agcggcttga agaccgggta atgcgtgcag
    10681 cccaggacca gcacatcggg agtttcggga tcggacgcgg gggcgggaag ccacccctcc
    10741 agataccgtt cgagcgtcgc ccgcgccacc gccgtcgttt cccagccttc ctcggccagc
    10801 ggcaccagca gcgggcaggc gcgcgagctg acccgcgccg cgggcgcgag ccggcggatc
    10861 gccgcctcat aggccccgct ggcgatcgtc gccgccgtgc cgatgacgcc gatccggccg
    10921 ctgcgggtgg cgccgaccgc cgccgccgcg cccggctcga tcaccccgac caccggcacc
    10981 ggcaggtccc gcagcgcctc cagcgcgaac gccgatgccg tgttgcaggc gatgaccagc
    11041 agatcgaccg cctcggccag cagcagacgc gtgttctgcc gggcatagtg gattacagtc
    11101 tggcgcgatt tgatgccgta cgggacgcgg gcggtgtcgc ccagatacac ggtggcatgg
    11161 cccggccagc gccgccggat ggcgcgcagg accgtcagcc ccccgatgcc gctgtcgaag
    11221 acgcccagcc tcatgggacc tcggcgccgt cgatcgcctg gccggtgatt ttttcgaaga
    11281 tctcgcggta gcgggccgcc gtgttggcca ccacctgcgc cggcagcggc ggcggcggcg
    11341 gctgtttgtt ccagtccagc gtctccaggt aatcgcggac gtactgcttg tcgaacgaga
    11401 tcggctcgcc gcccgcttcg aacgagccct tgacccagaa ccgcgacgaa tcgggcgtca
    11461 gcagctcatc gatcagaatg atctcatcgt cgacccaacc gaactcgaac ttcgtatcgg
    11521 ccaggacgat gccgcgctcg gccagatact cgtgcgcata acggtagagc cgcagactgt
    11581 agccgcggag cttctccgcg gtctcggcgc cgacggtctc ggcgca
//
LOCUS       DAOPCO010000114        11545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5497_length_11545_cov_4.639252, whole genome shotgun sequence.
ACCESSION   DAOPCO010000114 DAOPCO010000000
VERSION     DAOPCO010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11545
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5497_length_11545_cov_4.639252"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="PLS90_09350"
     CDS             <1..353
                     /locus_tag="PLS90_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02650.1"
                     /translation="SMGADGFQRYPGADGSLLGKGSLKDSFILGNLLTTIPPGTLLIA
                     AHHSPTLNQVFLHAFALARNTSPQLGGLHLIRLGEAVPPLAELPNPGICRDLDGPVEL
                     VLPLLLGAVFSLIE"
     gene            complement(1082..1708)
                     /locus_tag="PLS90_09355"
     CDS             complement(1082..1708)
                     /locus_tag="PLS90_09355"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPK02651.1"
                     /translation="MAPESRLLLFDFDGVVVDSYRAFFDAYTAACRAIARPGLADEQT
                     FLGVFDSNMLTGLKRLGIEGPALARFIDTLKRELTPRMPSVRCFDGMLDLLAEAARRH
                     PTRILTSNLTALVTEYLQSQGLAAIPPVLGVDVEPSKVAKIRLARAQEPALTACYIGD
                     TAGDMLEGREAGAIPVGVAWGWHDPSRLRAAGAQIIVTRPEDLLNLAL"
     gene            complement(1722..3806)
                     /locus_tag="PLS90_09360"
     CDS             complement(1722..3806)
                     /locus_tag="PLS90_09360"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPK02652.1"
                     /translation="MKVYETSRIRNVALAGHTGAGKTSLGEAMLYTLKQTDRLGKTDD
                     GNTVSDYDAEEIRRHHSIQVSLLPVEHDGAKINLLDLPGFRDFVGEIKSGLRAADSML
                     LVFDANGGVETGAETVHAYAVDMNRPRAYFINMLDKEHASYAKAFDALRAAFEPRMVR
                     LTLPVGEGLEFKGVIDLMRMKFVKEDGQKVSYEEIPADLLPEAQTAHAELIEIAAEGD
                     EELLEKFLGEETLSPEEILKGLSENFAEGGIVPVLCGSATALKGIRPLLDFLAHCAPD
                     PTHTAYEYTEGKSEEIKTITPSPDGSALLWVFKTVADPFIGRMSYFKVLRGSARKDGT
                     YHNVNHNSNEKFAHPLICKGKKSEEVDEVAAGDLGAVAKLDATRTGDTLVEPGTPDLQ
                     VTAVTIPKPTAFAAITAKSRADEDKVGIGFHRLMEQDPTLSLYRDPTISQTILSGMGE
                     THLQVAVARLKDLVKVEVDMEIPRVPYRETITRKNEGQGKYKKQTGGHGQYGDCWIRF
                     EPLPEGSGFEFEWAIVGGVIPTNFKSAVEKGLLESLEKGVLSGCPTVDIKATCYDGSY
                     HAVDSSDMSFKVAASLAFKNIIPKCAPIILEPIYKATISVPEEYMGDVMGDLNSRRGR
                     ILGMNQEGRRQIIEAHVPLAELYTYGRQLNSLTRGRGVYEMEFDHYERVPNDVQEKIV
                     AEYAQRREAEAE"
     gene            4299..5303
                     /locus_tag="PLS90_09365"
     CDS             4299..5303
                     /locus_tag="PLS90_09365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015111117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02653.1"
                     /translation="MVVSTSYPGSEVAARGASAIELSVVIPVYNEAENLRELHRRLTA
                     ALEPLALEYEILLVDDHSRDETPRIILELATADRRVRSLRFSRNFGHQMALSAGLRYA
                     RGEIVVMMDGDLQDPPEVLPDLLRKIRDEDWDVVYAIRRRRKESLVYRTLYKGFYRIL
                     SRMSYIDIPVDAGDFCAMRRCVADALNALPECNRFLRGLRAWVGFRQTGLEYERQARH
                     AGEAKYTLAALLRLAFDGIFTFSYVPLRVAMWFGFVISSLGLLYAVYLVWARLVHHSF
                     TGVLTGWTTLVVAVMVLGGLQMMLIGIVGEYIGRIFEETKRRPPYIVHRFVNFEDEAR
                     "
     gene            complement(5332..5640)
                     /locus_tag="PLS90_09370"
     CDS             complement(5332..5640)
                     /locus_tag="PLS90_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02654.1"
                     /translation="MKMLGQITWTAALALFLTMAAPASAAGSDQPIGWIRTGGATGTT
                     KPITPRALQDDFGLQFTGGYNPSNQLERPATCAKPSGWLRIGGATGTTRPICRATPCD
                     "
     gene            complement(5931..6899)
                     /locus_tag="PLS90_09375"
     CDS             complement(5931..6899)
                     /locus_tag="PLS90_09375"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPK02655.1"
                     /translation="MELIRQLDDLQNLCRDGTSLCIGVFDGVHRGHQSLIHHAVQHAR
                     RRGLRSLAFTFEQHPLRLLAPPYAPRMLTTPLEKAELLAQTGVDLCLMQPFTPEFSRI
                     SAEDFMERIVHRLCRARHVTCGPDFRFGHRGAGTIDRLKEFGAQRGFQVEVIDALLDG
                     RLPVRSTRIRQLLGEGRLEDAMRLLGRPYRLSGRVVEGDRRGRQIGYPTANIQPPEEK
                     LIPGDGVYAVRVAVAPSDAESITGAATHHPAMLNIGVRPTFSGQGRTIEAHLFDFSGD
                     LYGHVVGVTFVARVRDEKPFDSPEALFAQLQADEAACRSLLDTNSP"
     gene            complement(6936..8111)
                     /locus_tag="PLS90_09380"
     CDS             complement(6936..8111)
                     /locus_tag="PLS90_09380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPK02656.1"
                     /translation="MTWPILYAVIFLLAFGLSAALCALARRVGLRIGLMDRPSGRKAH
                     AQPVAVTGGWGFFSTFLLLVLAGSLLAGPMAGLLPPGWDEVRRYMANIAGVRTEILAV
                     LAGATLLFALGAIDDVRPLGPRLKLTVQVLAVLPLLLAGITVRGFLPLPLGWLLSLFW
                     IVLLTNSFNLLDNMDGLSATIALVICAVLSVAALQGGERWLPALFLCLAGALAGFLCY
                     NFHPATIFMGDAGSLVLGYLMGVFSILVTYYEPSPAIPTGLPILMPLAIMGVPLFDTL
                     SVIFIRWRTRRPIMQGDRNHFSHRLLAMGFSVRQAAVAIALLTAATGLLALPLRRLAP
                     GPALLHLLALGLLFAVIIMLEIAGRRGAMPGTRADLAPNPPAPEHAALQPGRSDASG"
     gene            8446..9798
                     /gene="ftsY"
                     /locus_tag="PLS90_09385"
     CDS             8446..9798
                     /gene="ftsY"
                     /locus_tag="PLS90_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPK02657.1"
                     /translation="MLKWFRRKKQEEPVESAQAPEAPAAAEPASTDEGPSDQLLHDQP
                     IPDDLQRDEPATLGEFSPADAEQMEWIAPTESTPVELDAAALSVEMERDAFRSDAEAT
                     ETEASGVSPFEREADETDSVEAVAAEEPAPEPAPEKKRGFFLRLRDRLGKTRSSLVEA
                     VRGAVRLRGKVDETLLEEIEEILIQSDVGVQTTTRIVDQMRKAARRQGALSPDELLAL
                     FKAEIERILTENNRPLEFDRARPMIVLVVGVNGTGKTTTIGKIGKVLADQGKRVMMVA
                     ADTFRAAAADQLQVWAERTGSCIVRQSEGADPASVVYEALEGARRDTPDVILIDTAGR
                     LHTKTHLMEELSKIGRVIRKHYPEAPHETVLVLDATTGQNALNQLRLFNDACQLTGLI
                     MTKLDGTAKGGILIACKDALNLPIFKIGIGESAEDLRDFDAHDFAEALFGGGNGADGA
                     "
     gene            9841..10545
                     /locus_tag="PLS90_09390"
     CDS             9841..10545
                     /locus_tag="PLS90_09390"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPK02658.1"
                     /translation="MDKLTRQYVELTEVDRALAQTREQLERYPALLARLEKQVEQAGA
                     AIAEAEAELERARRDRRQAEKEVETLRERISKYLLQQTQVKTNKEYEAITHEIEATRT
                     RIDEWETRGLEYLELEDQAAERKRGSEAEVVRLREELGRERERIEGLMAEKREAVARL
                     EEEKRQRLAALAPDEAEDYELLNNRNPGSAAVAVQQGHCGGCNWALVPHTQQQVRQGT
                     EPVRCEHCRRFLYEPA"
     gene            10742..11065
                     /locus_tag="PLS90_09395"
     CDS             10742..11065
                     /locus_tag="PLS90_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02659.1"
                     /translation="MFVQLHLKHESEYQEGELVALAESIGKLLHTLEISRVRSLLLDE
                     QALLQLQANRYPAAAVEEPAGEEAEPEPAVVRLQVWCNRAQIEPHIVRGLPAHLMREV
                     KYTYV"
     gene            11136..>11545
                     /locus_tag="PLS90_09400"
     CDS             11136..>11545
                     /locus_tag="PLS90_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPK02660.1"
                     /translation="MEVIHDPAEYQQRTLAWRRDGLRHALVPTMGALHAGHYRLIEEA
                     RAHADRVSVSLFVNPKQFGPNEDLAKYPRTFEADRAACAVRGVDLLFAPDAEAMYPAG
                     FQTHVEVAGLSEPLCGAARPGHFRGVTTVVLKLLL"
BASE COUNT         2038 a         3189 c         4452 g         1866 t
ORIGIN      
        1 tgagcatggg cgccgacgga tttcagcgct atccgggggc ggacgggtcg ctgctgggca
       61 agggcagcct gaaggattcg tttattctgg gcaatctgct gacgacgatc ccgccgggga
      121 cgctgctgat cgcggcgcat cattcgccga cgctgaacca ggtctttctg catgcgttcg
      181 cgctggcccg caacacaagc ccgcaactgg ggggactgca tctgatccgg ctgggcgagg
      241 cggtgccgcc gctcgcggag ctgcccaatc cggggatctg ccgggacctg gatggacccg
      301 tggagttggt gctgccgttg ctgctggggg cggtgttcag tctgattgag tgaaggggga
      361 aaggggaagc gaagggcaaa agggacgtaa gggacaggaa ggacgcaagg gacatagggg
      421 acagggagga tttggttgcg gacggtcaga ggggcgaggc cggatccggc cgcacctttc
      481 aggggctggg ctcgtcgggg gcggcgacgc agggttccgg cctgcggccg cggaccctgc
      541 gctttattcc ggtcgtccgc tccgcggact gatggggtga acaagtcggg cccgccacgc
      601 acgaggttct cgctttaatc aactctcggg tgatgtcctg gattagcgaa agcttgaccc
      661 ttttcgttag tctagaaccc ttcacggcta tcgggcgtta tcctgaacgg aggataacag
      721 gtggctggcc ctatttgctg attattcgcc ccctgcgggg acggggatgt tgcgcatcgg
      781 catccccagg ttccgctgcg ctccacttgg ggctattcaa aatgccgccc cgctggggcg
      841 ctgtcgcgga cggcatctca tgaatgtgag gcagcgctca acggaaagag aacgaaattt
      901 tcgtacggga gtggatgttt agcatggcgg cggcttgtgc cggcaaggat gccggcggtc
      961 ccaggcggtg gttaaggaga cgatgggccg atttcgagag ccgtggacga aggacgagag
     1021 ccgagagccg aatttcaggg ttgccctggg agggatcacg aggacggggg acgagggacg
     1081 attacagggc gaggttcagg aggtcttcgg ggcgggtgac gatgatctgg gcgccggcgg
     1141 cgcggaggcg ggagggatcg tgccagcccc aggcgacgcc gacggggatc gcgccggcct
     1201 cgcgaccctc cagcatgtcg ccggcggtat cgccgatgta gcaggccgtg agcgccggtt
     1261 cctgcgcgcg cgcgaggcgg atcttcgcga ccttgctcgg ctcgacatcg acccccagga
     1321 ccggcgggat ggcggcgagc ccctgcgatt gcagatactc cgtgacgagc gccgtcaggt
     1381 tggaggtgag gatgcgcgtc gggtggcgac gcgcggcctc ggcgagcagg tcgagcatgc
     1441 catcgaagca gcggaccgac ggcatgcggg gggtgagttc gcgcttcagc gtgtcgatga
     1501 agcgggcgag cgccgggcct tcgatgccga gtcgcttgag gccggtgagc atgttgctgt
     1561 cgaacacgcc gagaaaggtc tgttcatcgg cgaggccggg gcgcgcgatg gcgcggcagg
     1621 cggcggtgta ggcgtcgaag aacgcgcgat acgaatcgac gacaacgccg tcgaagtcga
     1681 acagcagcag gcgggattca ggcgccatgg cctgagcgcc cctactcggc ctcggcttcg
     1741 cgccgctggg cgtactcggc gacgatcttc tcctggacgt cgttcgggac gcgttcgtaa
     1801 tggtcgaact ccatctcgta gacgccgcgg ccgcgggtga gggaattgag ctggcggccg
     1861 taggtgtaaa gctcggcgag ggggacgtgg gcctcgataa tctggcggcg cccttcctgg
     1921 ttcatgccga ggatgcggcc gcggcggctg ttcaggtcgc ccatcacgtc gcccatgtac
     1981 tcctcgggga cggagatggt ggccttataa atcggctcca gaatgatcgg ggcgcatttg
     2041 gggatgatgt tcttgaaggc cagcgaggcg gcgactttga acgacatgtc ggaggagtcg
     2101 accgcgtggt atgagccgtc gtagcaggtg gccttgatgt cgacggtcgg gcaaccggag
     2161 aggacgccct tttcgagcga ttcaaggagc cctttctcga cggcggactt gaagttggtg
     2221 gggatcacac cgccgacgat ggcccactcg aactcgaagc cgcttccctc gggcagcggc
     2281 tcgaagcgga tccagcagtc gccatactga ccgtggccgc cggtctgctt cttatacttg
     2341 ccctggcctt cgttcttgcg ggtgatcgtt tcgcggtagg ggacgcgcgg gatttccatg
     2401 tcgacctcga ccttgaccag gtccttcagg cgggcgacgg cgacctgcag atgggtttcg
     2461 cccatgcccg agaggatggt ctgactgatc gtggggtcgc ggtagagact gagcgtgggg
     2521 tcctgctcca tcaggcgatg gaagccgatg ccgaccttgt cctcgtcggc gcgggatttg
     2581 gcggtgatcg ccgcgaaggc cgtcggcttg ggaatggtca cggccgtgac ctggaggtcg
     2641 ggggttcccg gctcgacgag ggtgtcgccg gtccgcgtgg cgtcgagctt ggcgacggcg
     2701 ccgaggtcgc cggcggcgac ttcgtcgact tcctcgcttt tcttcccctt gcagatgagg
     2761 ggatgggcga atttctcgtt cgagttgtga ttgacgttgt gataggtgcc gtccttgcgg
     2821 gcgctgccgc ggaggacctt gaagtaactc atgcggccga tgaacgggtc ggcgacggtc
     2881 ttgaagaccc agaggagggc cgagccgtca ggggacggcg tgatcgtctt gatctcttcg
     2941 gattttccct cggtgtattc gtaggcggtg tgggtgggat cgggggcgca gtgggcgagg
     3001 aagtcgagca gcgggcggat gcccttgagg gcggtggccg agccgcagag gacggggacg
     3061 atgccgccct cggcgaagtt ttcgctcagg cccttgagga tttcctcggg gctgagcgtc
     3121 tcctcgccga ggaatttttc gagcaattcc tcgtcgccct cggccgcgat ctcgatcagt
     3181 tcggcatgcg cggtctgcgc ctccggcagg aggtcggcgg gaatctcttc atagctgacc
     3241 ttctgaccat cctccttgac gaacttcatg cgcatcagat cgataacgcc cttgaactca
     3301 agaccttcgc cgacggggag ggtcaggcgg accatgcggg gttcgaaggc ggcgcggagg
     3361 gcgtcgaagg ctttggcgta ggaggcgtgc tccttgtcga gcatgttgat gaagtaggcg
     3421 cgggggcggt tcatgtcgac ggcgtaggca tggaccgttt cggcgccggt ctcgacgccg
     3481 ccgttggcgt cgaagacgag gagcatgctg tcggcggcgc gcaggccgct cttgatttcg
     3541 ccgacgaagt cgcggaagcc gggcaggtcg agcagattga ttttggcgcc atcgtgttcg
     3601 acggggagga gcgaaacctg gatggaatgg tggcggcgga tttcctcggc gtcgtagtcg
     3661 gagacggtgt tgccgtcgtc ggtcttgccg aggcggtcgg tctgcttgag ggtgtagagc
     3721 atggcctcgc cgaggctggt cttgcccgcg ccggtgtgac cggcgagggc gacgttgcga
     3781 atccgggagg tttcataaac tttcaaggcg ccatctcctt cgaccgagag tgagtgggtc
     3841 ggttgcgggg gcatgccgtg ccgcccgtcc gcggatcagc ggcagggcat gaagtggtcg
     3901 agtatagcgg cgaagggcgc gggtggcaac gggattgtca tgtccgaaat gacggcggga
     3961 gggggaatcc gggagggggg atgggggatc gcgaaaggcg ggacagggga tcgggggatc
     4021 acgaaaggca cgaaaggacg cgaaaggaag gataattgga tcacccccgc cgcggaggtc
     4081 agggagatcg cagaggagca gcgagggatg atttccaggt tcgggaagac gggccgtcgg
     4141 atccaaagcg gcgccggcgc gttgcgttcc aacgctctgg tggaccacac ttctcaaacc
     4201 ggtgaatccc gcgacgtatg aacaaaagaa gattgcaatt tggcgggcgt tgactggtaa
     4261 tcagtccgtt ggggagagtt gtccgagggt cgtaccagat ggttgtcagc acttcgtatc
     4321 cgggttcgga agtcgcggcc aggggggctt cggcaatcga actgtcggtg gtgatcccgg
     4381 tctataatga agctgaaaac ctgcgcgagc tgcaccggcg gctgacggcg gcgctcgagc
     4441 cgctggcgct tgagtatgaa atcctgctcg tggacgacca cagccgcgat gagacgccga
     4501 ggatcattct ggagctggca acggccgacc ggcgggtgcg atcgctgcgc ttttcgcgca
     4561 atttcgggca tcagatggcg ctgagcgccg gcttgcgcta cgcgcggggg gaaatcgtcg
     4621 tgatgatgga cggcgacctg caggacccgc cggaagtact gcccgacctg ctgcgcaaga
     4681 tccgcgacga ggactgggac gtggtctacg ccatccggcg gcggcgcaaa gagagcctgg
     4741 tctaccggac gctctacaag ggattctatc ggatcctcag ccgaatgtcg tacatcgaca
     4801 ttccggtgga cgcgggggat ttctgtgcga tgcgccgctg cgtcgccgac gcgctgaacg
     4861 cgctgcccga atgcaaccgc tttctgcgcg gcctgcgcgc gtgggtcgga tttcgccaga
     4921 cggggttgga gtatgagcgg caggcgcgcc acgcgggcga ggcgaaatac acgctggcgg
     4981 cgctcctccg gctcgccttc gacgggatct tcacgttttc ctatgtcccg ctgcgcgtgg
     5041 cgatgtggtt cggtttcgtg atctcgtcgc tgggattgct ctacgcggtc tatctggtgt
     5101 gggcgcggct ggtgcatcac agcttcacgg gggtgttgac gggctggacg acgctcgtcg
     5161 tcgcggtaat ggtgctgggg gggctgcaga tgatgctgat cgggatcgtg ggggagtaca
     5221 tcggccggat cttcgaggaa accaagcgcc ggccgcctta catcgtccac cggtttgtga
     5281 attttgagga tgaggcgcgg tgaggggtag aggccgttcg cggcggaggc ctcaatcgca
     5341 cggggtggcg cggcagatcg gtcgggtcgt gccggtcgcg ccgccgatgc ggagccatcc
     5401 agagggcttg gcgcacgtgg cggggcgttc gagctggttg gatggattgt aacccccggt
     5461 gaactgaagg ccaaagtcat cctggagagc tcgcggggta atgggctttg tcgttccggt
     5521 cgcaccaccc gtgcggatcc agccaatcgg ctgatcagat ccggccgccg aggcgggcgc
     5581 cgccatggtc aggaacaggg ccaatgcggc cgtccatgtg atctgtccga gcattttcat
     5641 ctccagtttc cttccgttga tgaggtattc cgccgaaggc gttcgggtca tacgggatgc
     5701 acggatcaag ccatttcggt ggggtgggtc cggtttttgg ggaagggttg agtttattcg
     5761 gtttcctatc gattttgtat ggcgtcctaa gtatgtcggc gggcatcgga gctggtttag
     5821 caacggcggg ctgtggcaaa tgcaacagat ttggggatgg aatgcggagg aaaccccggc
     5881 gggggcgcct ctcccacacc ggcactggcc cccacaccgg ctccggatgt tcagggggaa
     5941 ttggtatcga ggagcgagcg gcaggcggct tcatcggcct ggagctgggc gaagagggct
     6001 tcaggggagt cgaagggctt ttcatcgcgc acgcgcgcga caaaggtgac gccgacgacg
     6061 tggccataga gatcgccgga gaaatcgaat agatgggctt cgatcgtgcg cccctggccg
     6121 ctgaaggtcg gccggacgcc gatgttcagc atggcggggt gatgcgtggc ggcgccggtg
     6181 atggattcgg cgtcggaagg ggcgacggca acgcgcacgg catagacgcc gtcgccgggg
     6241 atcagctttt cctccggcgg ctggatgttg gcggtggggt agccgatctg gcgtccgcgg
     6301 cggtcgccct cgacgacgcg gccggaaagg cgataggggc ggccgaggag gcgcatggcg
     6361 tcctccagcc ggccctcgcc gagcaactgg cggatgcggg tcgagcggac ggggaggcgg
     6421 ccgtcgagga gcgcgtcgat cacctcgacc tggaagccgc gctgggcgcc gaattccttc
     6481 aggcggtcga tggtgccggc gccgcgatgg ccaaagcgga aatcgggccc gcaggtgacg
     6541 tgacgcgcgc ggcagaggcg atggacgatc cgctccatga aatcctcggc cgagatgcgc
     6601 gagaactccg gcgtgaaggg ctgcatcagg cagaggtcga cgccggtctg cgccagcagt
     6661 tcggccttct cgagcggcgt cgtcagcatc cggggggcgt aggggggggc gagcaggcgc
     6721 agtggatgct gctcgaaggt gaaggcgagc gagcgcagcc cgcggcggcg cgcatgctgg
     6781 acggcatgat ggatcagcga ctggtggccg cggtggacgc cgtcgaagac gccgatgcac
     6841 agactggtgc cgtcgcggca gaggttctgc aggtcgtcaa gctggcggat cagttccatg
     6901 ggtggcgctc ctttgcgcgg cgcggctgac cggcctcagc ccgaagcatc cgaccggccg
     6961 ggctgaagcg ccgcgtgctc cggggccggg ggattcggcg cgaggtccgc gcgcgtgccg
     7021 ggcatcgcgc cgcggcggcc ggcgatttcc agcatgatga tcactgcgaa gagcaacccg
     7081 agggccagca gatgaaggag cgccggaccg ggggcaaggc ggcgcagagg gagcgccagc
     7141 agtccggtgg cggcggtcag cagcgcgatc gcgacggcgg cctgccggac ggagaaaccc
     7201 atggcgagca accgatgcga gaaatgattg cggtcgccct gcatgatcgg gcgccgcgtg
     7261 cgccagcgga tgaagatcac ggagagcgta tcgaacagcg gtacgcccat gatcgccagc
     7321 ggcatcagga tcgggagccc tgtcgggatt gcagggctcg gctcatagta ggtcaccaaa
     7381 atggagaaga cgcccatcag gtagcccagc acgagactgc cggcatcgcc catgaagatc
     7441 gtcgcgggat gaaaattgta gcaaagaaag ccggcgagcg cgcccgcgag acacaaaaac
     7501 agggccggca gccagcgttc gccgccctgg agcgcggcga cggagagcac ggcgcagatc
     7561 accagcgcga tggtggcgct gaggccgtcc atgttgtcga gcaagttgaa ggagttggtc
     7621 agcaggacga tccagaaaag gctgaggagc cagcccagcg gcaggggaag gaagccccgg
     7681 accgtgatgc cggccagcag gagggggagg acggccagga cctgaacggt gagcttgagg
     7741 cgggggccga gcgggcgtac gtcgtcgatt gcgccgaggg cgaacagcag ggtcgcgcca
     7801 gccaggacgg cgaggatttc cgtccgcacg ccggcgatat tggccatgta gcggcggact
     7861 tcatcccagc cgggagggag gagtccggcc atcggccccg cgaggaggga gccggccagc
     7921 acgaggagca gaaaggtgga gaagaaaccc cagccgccgg tgacggcgac cggttgggca
     7981 tgggctttgc ggcccgaggg gcgatccatg aggccgatgc gcaagccgac gcgtcgggcg
     8041 agggcgcaga gcgccgccga caggccgaag gcgagcagga aaattacggc atagaggatc
     8101 ggccaggtca tctcgcgtcg gggtctcgcg cgggagtctg tcgatgttcg acttggctga
     8161 agcttcgcag atgccggcgc gattcgcaag caggggccgc cggggattga ggatgaggga
     8221 cgagagccgg gagccgtatt ctggactggg ccgctggggg cgaacacgcg gacgaggacg
     8281 agggacgagg acgagggacg agagacgggg gggcgaatgg ggttgttggc tttggatggg
     8341 aggggtataa gtggcggggg gctgcccggt cggcagggat ggaatgggac cggcgccgac
     8401 gcggaacgcg acccgatccg gatgatccgc cccgaggagt ggatgatgct gaaatggttt
     8461 cgtcgcaaga agcaggagga gccggtggag tcggcgcagg cgcctgaagc gccggcggcc
     8521 gccgagcccg cctccacgga cgaaggcccg tcggatcagc ttctgcacga ccagccgatt
     8581 ccggacgacc tgcaacggga tgaaccggcg acgctggggg aattcagtcc ggccgacgcc
     8641 gagcaaatgg agtggatcgc gccgacggaa tcgacgccgg tggaactgga cgcggccgcg
     8701 ctgagcgtgg agatggaacg cgacgccttc cggagcgacg cggaagcgac cgagaccgaa
     8761 gcgtcaggag tcagtccgtt tgaacgggaa gcggacgaga cggattcggt cgaggcggtg
     8821 gcggcagagg aaccggcgcc cgagccagcc ccggaaaaaa agcgcgggtt cttcctgcgc
     8881 ctgcgcgacc ggctgggcaa gacgcgcagc tcgctggtgg aggcggtccg cggggcggtg
     8941 cgcctgcggg gcaaggtgga cgagaccctg ctggaggaga tcgaggagat cctgatccag
     9001 agcgacgtgg gggtgcagac cacgacgcgg atcgtcgacc agatgcgcaa ggcggcgcgg
     9061 cgccaggggg cgctgtcgcc tgatgagttg ctcgcgctgt tcaaggcgga aatcgagcgg
     9121 atactgaccg aaaacaaccg gccgctggaa ttcgaccgcg cgcgcccgat gatcgtcctg
     9181 gtcgtcgggg tgaacgggac ggggaaaacg acgacgatcg gcaagatcgg caaggtgctg
     9241 gcggaccagg gcaagcgggt gatgatggtg gcggccgata cgttccgcgc ggcggcggcc
     9301 gaccagttgc aggtctgggc cgagcggacc ggctcgtgca tcgtgcgtca gagcgagggg
     9361 gcggacccgg cgtcggtcgt ctacgaggcg ctggaggggg cgcggcgcga cacgcccgac
     9421 gtgatcctga tcgacacggc ggggcggttg cacaccaaga cgcacctgat ggaggagctg
     9481 agcaagatcg ggcgggtgat ccggaagcat tatccggagg cgccgcacga aaccgtgctg
     9541 gtgcttgacg cgacgacggg gcagaatgcc ctcaatcagc ttcgcctgtt caacgacgcg
     9601 tgccagctta cggggctgat catgacgaag ctggacggga cggccaaggg cgggatcctg
     9661 atcgcctgca aggacgcgtt gaatctgccg atcttcaaga tcgggatcgg cgagagcgcg
     9721 gaggatctgc gcgactttga tgcgcacgat ttcgccgagg cgctcttcgg cggcgggaac
     9781 ggcgcagacg gcgcctgacg ggcggcgggc ggagtccggc ccgcgcggtg gggagaagcg
     9841 atggataagt tgacacgcca atatgtcgag ctgaccgagg tcgaccgcgc gctggcgcag
     9901 acgcgcgagc aactggagcg ctacccggcg ttgctggcgc ggctcgaaaa acaggtggag
     9961 caggccggcg cggcgattgc ggaggccgag gcggagctgg agcgggcgcg gcgcgaccgg
    10021 cgccaggccg agaaggaagt ggaaacgctg cgtgagcgca tttccaagta cctgctgcag
    10081 cagacccagg tcaagacgaa caaggaatac gaggcgatca cgcacgagat cgaggcaacg
    10141 cgcacgcgga tcgatgagtg ggaaacgcgc gggcttgagt accttgaact ggaggatcag
    10201 gcggccgaac ggaagcgcgg aagcgaggcg gaggtcgtcc ggctgcgcga ggaactgggc
    10261 cgcgaacggg aacgaatcga gggcctgatg gccgagaagc gggaggccgt ggcacggctg
    10321 gaggaggaga agcgccagcg gctggcggcg ctcgcgccgg atgaggcgga ggattacgag
    10381 ctgctcaaca accgcaatcc cggctcggcg gccgtggccg tgcagcaggg gcactgcggc
    10441 gggtgcaact gggcgctcgt gccgcacacg cagcagcagg tgcgccaggg caccgagccg
    10501 gtccgctgtg aacattgtcg gcggtttcta tacgagccgg cgtgaggggt ttcggcgggg
    10561 atacgcggcg gtcactggct gaggcccggc accaataaca ggaccccaaa ggggccagaa
    10621 gaatgcccgc tggcgcaggc tcccaaggcg cggaaacatg gagggcaagg ccactgtgga
    10681 gagtggcgtt cggctttcga acaccttgga aaagtccccg gaccgcggag gaccgcatgc
    10741 catgttcgtt caactccatc tgaagcacga gagcgaatac caggaagggg agctggtcgc
    10801 gctggccgaa tcgatcggca agctgctgca cacgctggag atctcgcgcg tccgctcgct
    10861 gctgctcgat gagcaggcgc tgctgcagct ccaggcgaac cggtatccgg cggcggcggt
    10921 cgaggaaccg gccggcgagg aggccgaacc ggaacccgcc gtggtgcggt tgcaggtgtg
    10981 gtgcaaccgg gcgcagattg aaccgcacat cgtgcgcggg ctgcccgcgc atctgatgcg
    11041 cgaggtcaaa tacacctacg tctgacgggc ggggggccgt ggacgattgc gagggcgata
    11101 ccgaaaccga ttgttggggg cggggcaagg gcgcgatgga agtcattcat gatccggcgg
    11161 aatatcagca acggacgctg gcgtggcggc gcgacgggct gcgccacgcc ctggtgccga
    11221 cgatgggcgc gctgcacgcc gggcattacc ggctgatcga ggaggcgcgc gcgcacgccg
    11281 accgggtcag cgtgagcctg ttcgtcaacc cgaagcagtt cgggccgaac gaagacctgg
    11341 caaagtatcc gcggacgttt gaggctgacc gcgcggcatg tgcggtgcgc ggggtggacc
    11401 tgttgttcgc gccggatgcc gaggcgatgt atccggcggg gtttcagacg cacgtggagg
    11461 tggcggggct gagcgagccg ctgtgcggag cggcgcggcc ggggcatttc cgcggggtga
    11521 cgacggtggt gctcaagctg ctgct
//
LOCUS       DAOPCO010000115        11532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5507_length_11532_cov_4.906509, whole genome shotgun sequence.
ACCESSION   DAOPCO010000115 DAOPCO010000000
VERSION     DAOPCO010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5507_length_11532_cov_4.906509"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            551..1552
                     /locus_tag="PLS90_09405"
     CDS             551..1552
                     /locus_tag="PLS90_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK02661.1"
                     /translation="MVNIKNVADRAGVSIATVSHVLNQTRRVRPETAARVRTAIEELG
                     YSQNLAARNLATGRSNILGLIVSDIQNPHFPELTRNFQDQALLHNMEVLVMNTGYDPQ
                     RTSDCVRRLIGLRVPGLAIMTTEYDTGLVELVARQNIRAVYYFNPGPLSPLISCILVN
                     YGEGIREAVNHLEELGHRQIAFIGGAPHLQSAMYRRQAFIEQMQARGEGAMRIVDADF
                     SAEGGYYGCSRLLSSAPPTAIVCANDLMAFGAMHCCRDKGLRIPQDISIVGFDNISFS
                     QYSHPPLTTVAIPRDKLGQLAFKALWEMINRQTSEGRVIQLGTRLLVRESTAPPRKN"
     gene            1779..1970
                     /locus_tag="PLS90_09410"
     CDS             1779..1970
                     /locus_tag="PLS90_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HPK02662.1"
                     /translation="MARLKEGQDYYRDQNGLLVFTADYLRRRGFCCHCGCRNCPYPET
                     EALPTSATAVAAESPVSVK"
     gene            complement(2030..3850)
                     /locus_tag="PLS90_09415"
     CDS             complement(2030..3850)
                     /locus_tag="PLS90_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HPK02663.1"
                     /translation="MTSILGISAYYHDSAACLVIDGEIIAAAQEERFSRVKHDERWPA
                     QAIAYCLKEAGLRPEALDAVVFYEKPLIKFDRILETFLAEAPRGLKMFMRGLQLWSTQ
                     RLHIAAEIQRRIGEEWRGKLLFTEHHQSHAASAFYPSPFEEAAILTIDGVGEWATTTI
                     AHGKGRELKILKEIHFPHSLGLLYSAFTYFCGFRVNSGEYKLMGLAPYGEPRYVDLIH
                     EHLITVGEDGAFRLNLKNFRYTRGDTMTGEGFIRLFGGPPRRPETPITQREMDLAASV
                     QQVTEEIMVKLAATAVRETGCRKLCLAGGVGLNCVGNGRILRAGVVDDLWIQPAAGDA
                     GGALGAALDVWHNFYLKPRTPRAGDAMRGSRLGPRWRDEEIAERLRPRGAVMHWTAPE
                     ELPDRVAELIERENVIGWFQGRMEFGPRALGSRSILGDARSPRMQSVMNLKIKFRESF
                     RPFAPCVLAERVSDWFELDRESPYMLLVAPVLEKHRLAPDASGGLRGLDRLKARRSTV
                     PAITHVDYSARIQTVDAERHGVLYDVMKAFERRTGCPVIINTSFNIRGEPIVCRPEEA
                     YRCFMLTDMDALVLGHWVLLKHEQPPMEGAEEYRRQFKLD"
     gene            complement(4213..4680)
                     /gene="dut"
                     /locus_tag="PLS90_09420"
     CDS             complement(4213..4680)
                     /gene="dut"
                     /locus_tag="PLS90_09420"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPK02664.1"
                     /translation="MPATMSTVQIQIARSPAAADLPLPQYATEGAAGLDLRAAVPADQ
                     PFALAAGAWGIVPTGLRVAIPPGYEGQVRPRSGLALRDGIGILNGPGTIDSDYRGEIG
                     IILFNFSDRDFLIRRGDRIAQLVIQRVERCEWKEVETLPDTTRGPGGFGHTGR"
     gene            4895..6964
                     /gene="ligA"
                     /locus_tag="PLS90_09425"
     CDS             4895..6964
                     /gene="ligA"
                     /locus_tag="PLS90_09425"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPK02665.1"
                     /translation="MSNSRLSVEEAAIRIESLREQIRHHDELYYRHAAPEITDREYDA
                     LVDELRELEKRFPQFSSEASPTETVGSDRAEGFETVVHPVPMLSISNTYTPGEVREFD
                     ERIRRLLGRGEKEAIPYVVELKIDGVAVTIMYQDGRLEYAATRGDGVRGDLITANIRT
                     IHKIPARLPEGAGLAPRGRFEVRGEVFMRHGEFQKLNQQRIELGLEPFANPRNTTAGT
                     LKLLDARLVAERPLDIFLYAVGAHDAPLPARHWEVLELLDRLGLPTNPERRRCENLDE
                     VLAAIESWETARKELDYDTDGLVIKVDELALREELGARSKSPRWMIAYKFSAEQAQTR
                     LHGIELQVGRTGAVTPVAHLEPVFVAGSTISRATLHNADEIERKDIRVGDTVIIEKGG
                     DVIPKVVRVIESLRTGQEAPFVYPTECPVCGGGLHRPEGEAVHRCINASCPAQVKGRI
                     LHYASRNAMDIDGLGDKIVDQLVDGGHVEDIAGLYELTREQLAGLERMAEKSADNLLR
                     GIDESRRRPLANLIYALGIRFVGSAGARLLVRRYASIDALIAAGEEELTAIDGVGEVV
                     ARSVREFFAEEKNRRLIERLRMHGVNLERLPEEAPPSAEALAGSPFAGKTCVLTGKLE
                     TMSREEGEERIAALGGKAAGSVSKKTDLVIAGPGAGSKLEKARQLGIEIIDEAEFIRR
                     LEAAAGG"
     gene            complement(6989..10897)
                     /locus_tag="PLS90_09430"
     CDS             complement(6989..10897)
                     /locus_tag="PLS90_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02666.1"
                     /translation="MIRGIKDRRDKRLRERLRDEQGLLAGALRDPTIREQVIDLCLAD
                     PDLLRDILHSEPAIDQILRDRVLLDRILPTEEAFHRLIENDAQLSLILHHPRSAEKLL
                     ADPLLLHRLLGDDRTLAALLEHEDLLRRLLADDRLAALIADDTRLLHLLLRDRRALPL
                     LLENEELLSRIITSPQGQARIFAHEALLHLILADEKSLRALVGQPELLDRLLAREETF
                     DRLLGQSDWIDRFLEHPAVLDRLLQLPGALDRLLTHDAVLSRFAQHPDLLDRLIAHDD
                     FIACLAEQPDRLERLLRHGPLLNVILDREAFLGRLLSDPRLLARALATPAFVERLVSS
                     DPALDFIFRPGGLLELMLQRGQGVNRLGDRAELLDALLDSPPLLARLHARDDLLSRLS
                     RDERLQRAILRDEQTFVTLLRRPAAREPLARAARDLLPLLLESEPAFALLQADAELRR
                     RLLRDEDALARVLASPALLEAVLDSPAAFPRLAVKPALLDRLFGHEAGWSALLRRPEL
                     IDRLLATETAFQALAGKTGLLRRLLAHPAARRLILDDDALVAELLLHPPVVERLAGDE
                     TFLQSLLRPERPFLGALLRRSEALAHLRSDPQWLDIALSEPALLRRLAARDDLLQAML
                     DDPTARDRIFQRHEWLRGLLRDPRMLPLILADGALLDRIVAAPRAHERVLANFEILDR
                     LLAEDLALDRLVKHSFMLDRLLARPAVLRRALELKPFLHSVIASAETVDAIADLPAAL
                     DNLLDDPRVHEFLLSHPARLERLLRDPRTQERIVHLPDVLEALLAHERTVSRLLAHGE
                     LLDRLAGDDGLLQRVLQRDAALDRLLGEERLIARLLAHPVALGAILAHPATLESIREN
                     PILLGRLLRHDDVLQFITGDPRLVGRLLQSEFVVARLLSARDTLPVLASALQAHGGSP
                     LLADLAADDEILARRLLGDPRFLERILRDDRLCTLIIMERLVLDDRLLDRLPIDERLL
                     QKLLARDSVVERILADRRVQERMADDPRLLSHRPGADAPQPRADQLRLLADPSSPIAV
                     QLEFDRLWELLRPHARADRVTDERLAEARSRLRPGDDPARALLEVIRADGVLRLRQGD
                     LAIPDDEDPWPPLVATLIARAWDFDADTDAPVVLDVGAGHGLAIYHDKSRFPNARIIA
                     FEPDPRRHALAVENARRLLWRDVEIHPFLPTGGRTGARKRDRAPDHRRLSHYLRGPVH
                     LLRLRVPAGEADLLRDARRRLGLVRAVIVELRLAPGESGNRLRKVLNVLSKAGFEVRV
                     GQPVAAQRLDPAAALHVAGGDVTRVYARNRRPVAGLSAAD"
     gene            11207..>11532
                     /locus_tag="PLS90_09435"
     CDS             11207..>11532
                     /locus_tag="PLS90_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02667.1"
                     /translation="MATLVCSLSGCTSEKPSSESGSSKPDLLVMETPVLPEVEPPEVI
                     AEPEEAESTPTDSLTTGATGDEAEAPVGFERNIQDFPANLLQNPSFEAWEDSLVGWQV
                     TGAEVAA"
BASE COUNT         2014 a         3472 c         4250 g         1796 t
ORIGIN      
        1 ccagcagagc cagcagccca ccgagcagcg gccaaagtaa caggtaatcc atgtgtgggg
       61 tctcccgacg gccggacagg tccgcaggtc agtgcctcgg cgccggccgg atatggcgtg
      121 acgtgggacg agtcccgtga tctcggccca cgaagcggcc cgacgggaag ggactcacgt
      181 cgggcgtctg ccggtccaag gggacacggc tagacaaagc caaaacccta acatcgcgct
      241 tgggaatcgc aagagaaaag agcaaacagg gtccgatgca ggctccttga ttgcgaacga
      301 acgggggcgg catcgccgca agcggcattt tgcgattttt caggattcag cagaaaatcg
      361 cgtacattga gtgcaggttt ttcggaagtt gttcatgatt ttgacttcgc ggagatcgag
      421 aaatagaatc tgaaaggaca taaaggacgt aaaggacaat aggacgttag gggggtagta
      481 tgatgaaatg accggttttc gagagccgag gacgaaacgg gagacgggat tcagggttag
      541 ggggcgagag ttggtcaata tcaagaatgt agctgaccgg gcgggcgtct cgatcgccac
      601 cgtctcccat gtgctcaatc agactcgccg ggtgcggccc gagacggccg cgcgcgtccg
      661 gacggcgatc gaggagctgg gctactccca gaaccttgcc gcgcgcaacc tggccaccgg
      721 ccgcagcaac atcctgggcc tgatcgtctc cgacatccag aacccgcatt tccccgagct
      781 gacccgcaac ttccaggatc aggcgctgct gcacaacatg gaagtgctgg tgatgaacac
      841 cggctacgac ccgcagcgga cgtcggactg cgtccggcgg ctgatcggcc tgcgcgtgcc
      901 gggtctggcg atcatgacga cggaatatga cacggggctg gtcgagctgg tggcgcgcca
      961 gaacatccgc gcggtgtact atttcaatcc ggggccgctc agcccgctga tcagttgcat
     1021 tctggtgaac tatggcgagg ggatccgcga ggcggtcaat catttggagg agttggggca
     1081 tcggcagatc gcgttcatcg gcggcgcgcc gcacctgcag tcggcgatgt atcggcggca
     1141 ggcattcatc gaacagatgc aggcgcgcgg cgagggggcg atgcgcatcg tcgatgccga
     1201 cttcagcgcc gagggaggct actacggttg ctcgcggctg ctgagttccg cgccgccgac
     1261 cgcaatcgtc tgcgccaatg acctgatggc gttcggcgcg atgcactgct gccgcgacaa
     1321 gggattgcgg ataccgcagg acatctcgat cgtcggcttc gacaacatca gcttctcgca
     1381 gtacagccat ccgccgctga caaccgtcgc catcccgcgc gacaagctgg ggcagttggc
     1441 cttcaaggcg ttgtgggaga tgatcaaccg ccagacaagc gaggggcgcg tgatccaact
     1501 ggggacgcgg ttgctggtgc gggaatcaac ggcgccgccg cgcaagaatt aggcggcgga
     1561 catcccgggg gtgttcgcca atccggtcta tcgcacctca cacgatgcgg ggtcctggaa
     1621 gccgtgcaat gtcgttccgg actgcggggt ccggctccgg gtttttgctt gaagcgccgg
     1681 cgcggctggg gtagatatac atgagttgtt tgtcagttaa gcgcactcgc cgcgacacgc
     1741 cgcccgattt ccccacagat gcggaaccgc ttggagtcat ggcccgattg aaggaaggac
     1801 aggactacta ccgcgaccag aacgggttac tggtgttcac ggcggactac ctgcggcggc
     1861 gcggcttctg ctgccattgc ggctgtcgca actgccccta cccggaaacc gaggcgctgc
     1921 cgacgtcggc gacggccgtc gccgccgagt cgcccgtctc cgtcaagtga tcgatccgtc
     1981 ttccctcggc atccattcag attaatcggc gcttaccggg gcgatcgcgt caatccagct
     2041 tgaactggcg gcggtattct tcggcgcctt ccatgggcgg ctgctcgtgc ttcagcagca
     2101 cccagtggcc cagcaccagg gcgtccatgt cggtcagcat aaagcagcga taggcctcct
     2161 cgggccggca gacgatcggt tcgccgcgga tgttgaacga ggtgttgatg atcaccgggc
     2221 agccggtgcg ccgctcgaac gccttcatca cgtcgtagag cacgccgtgg cgttcggcgt
     2281 ccaccgtctg gatccgcgcg gaataatcga cgtgcgtgat cgcgggcacg gtggagcgcc
     2341 gggctttcaa ccggtccagt cccctcagcc cgccgctggc gtccggcgcc aggcggtgct
     2401 tttccagtac gggcgccacc agcagcatgt agggcgattc gcgatcgagc tcgaaccagt
     2461 cgctgacgcg ctcggccagc acgcaggggg cgaacggccg gaacgactcg cggaacttga
     2521 tcttcagatt catcaccgat tgcatgcgcg ggctgcgggc atcgccgagg atcgagcggc
     2581 tgccgagcgc gcgagggccg aattccatcc gcccctgaaa ccagccgatc acgttttccc
     2641 gctcgatcag ctccgcgacg cggtcgggca gctcctccgg ggcggtccag tgcatcaccg
     2701 cgccgcgggg ccggagccgc tccgcgatct cctcatcgcg ccaccggggg cccagccggc
     2761 tgccgcgcat ggcgtcgccg gcgcggggcg tgcggggttt gaggtagaag ttgtgccaga
     2821 cgtcgagcgc cgcgccgagt gcgccgccgg catcgcccgc ggcgggctgg atccacaggt
     2881 cgtcgacgac gccggcgcgc aggatcctgc cgttgccgac gcagttgagt ccgacgccgc
     2941 cggcgaggca cagcttgcgg caaccggttt ccctgacggc cgtggcggcc agcttgacca
     3001 tgatttcctc ggtgacctgc tggaccgagg cggccaggtc catttcgcgc tgggtgatcg
     3061 gcgtctcggg ccggcggggg gggccgccga acagccggat gaagccctcg ccggtcatcg
     3121 tgtcgccccg cgtgtagcgg aagttcttca ggttgaggcg aaacgcgccg tcctcgccga
     3181 ccgtgatcag gtgctcgtgg atcaggtcga cgtatctggg ctcgccgtag ggcgccaggc
     3241 ccatcagctt gtattcgccg ctgttgacgc ggaagccgca aaagtaggtg aaggcggagt
     3301 acagcagccc cagcgaatgg ggaaagtgga tctccttgag gatcttgagt tcgcgcccct
     3361 tgccgtgggc gatcgtcgtc gtcgcccatt ctcccacgcc gtcgatcgtc agaatcgccg
     3421 cctcctcgaa cggcgaggga tagaacgcgc tggcggcgtg ggactgatga tgctcggtga
     3481 agagcagctt gccgcgccac tcctcaccga tccggcgttg gatttccgcg gcgatgtgga
     3541 gccgctgcgt tgaccagagc tgcaggccgc gcatgaacat cttcaggccg cgcggcgcct
     3601 cggcaaggaa ggtctccagg atccggtcaa acttgatcaa tggcttttca tagaacacga
     3661 cggcgtcgag cgcctccggc cgcaaacccg cttccttcag gcagtaggcg atggcctgtg
     3721 ccggccagcg ctcgtcgtgc ttgacgcggg agaatcgctc ctcctgggcg gcggcgatga
     3781 tctcgccgtc gatcaccagg caggccgcgc tgtcgtggta ataagccgaa atgccgagaa
     3841 tggaagtcat cgccgcgccg tcacgtgcct gccctcaccg tcggttcgag ctgttctgcc
     3901 gtccctatgg gactctttcg ttctggcttc ccgattcccg ccacggaagt ggcgggctga
     3961 aatctgccgt ccctggcgtg accggcgtgc cgattggtcc gggaaatcct tcggccgttc
     4021 gaatcggccg ttccttgttc catccttcct tggggaccgt cggttcttga gcaactcatg
     4081 atcgatccgc ggaccgtcat gcgcgctggg gacgcgctcc atggtcctgt ccatcatagc
     4141 gtccggccgt gcgcgtgcga tattccaaat cgatcggcgt ttgattggga aatcgggccg
     4201 gggacggcgg ggtcagcgac cggtgtgacc gaaaccgccg gggccgcgcg tcgtgtcagg
     4261 gagtgtttcg acttccttcc attcgcagcg ttcgacgcgc tggatcacaa gctgcgcgat
     4321 gcggtcgccg cgccggatca ggaagtcgcg gtccgaaaaa ttgaacagga tgatgccgat
     4381 ctcgccgcgg taatccgaat caatcgtgcc cggtccgttg aggatgccga tgccgtcgcg
     4441 cagggccagg cccgagcggg ggcgaacctg cccttcgtat ccgggaggaa tcgccacgcg
     4501 caggccggtg gggacgatgc cccaggcgcc ggcggcgagc gcaaacggct ggtcggcggg
     4561 gacggcggcg cgcaggtcga gccccgccgc gccctcggtt gcgtattgcg gcagcggcag
     4621 atcggccgcc gcgggcgaac gcgcgatctg gatctggacg gtcgacatgg tggcgggcat
     4681 cggttctctc tttcatttcc ggtccggaac gggtcccgct atactctagc cgcgcggaag
     4741 cgtggttgaa accgtcaatt ttcaggagag acaactgggg ggctgatcgc gagttagtca
     4801 tgaatcggcg gatttcagag aggggagaaa cagcaagatt tctagagccg aggacgagag
     4861 acgagggacg gattatgagc gagggacagt gcgtatgagc aactcccgat tatccgtgga
     4921 agaggccgcc atccggatcg aatcgttgcg cgagcagatc cggcatcacg atgagctgta
     4981 ttaccggcac gccgcgccgg agatcaccga ccgcgaatac gacgcgctgg tggacgagct
     5041 gcgcgagctg gagaaacgct tcccgcagtt cagcagcgag gcatcaccga cggagacggt
     5101 cggcagcgac cgcgccgagg ggttcgagac ggtcgtccat ccggtgccga tgctctcgat
     5161 cagcaacacc tacacgccgg gcgaggtgcg cgagttcgat gagcggatcc ggcggctgct
     5221 gggccgcggc gagaaggagg cgatccccta cgtcgtggag ctgaagatcg acggcgtcgc
     5281 cgtgacgatc atgtaccagg acgggcggct ggaatatgcg gcgacgcgcg gcgacggcgt
     5341 gcgcggcgac ctgattacgg ccaatatccg gacgattcac aagatcccgg cgcgacttcc
     5401 ggaaggggcc gggctggcgc cgcgggggcg attcgaggtg cgcggggaag tcttcatgcg
     5461 ccacggcgaa ttccagaaac tgaatcagca gcggatcgaa ctgggcctgg agccgttcgc
     5521 caacccgcgc aacacgacgg ccgggacgct caagctgctc gatgcgcggc tggtggccga
     5581 gcggccgctg gacatctttc tgtatgcggt gggggcgcac gacgcgccgc tgcccgcgcg
     5641 gcactgggaa gtgctcgagt tgctggaccg gctggggctg ccgaccaacc ccgaacggcg
     5701 gcgctgcgaa aacctggacg aggtgttggc cgcgatcgaa agctgggaga cggcgcgcaa
     5761 ggagctcgat tacgatacgg acgggctggt gatcaaggtg gacgagctgg ccttgcgcga
     5821 ggagttgggg gcgcggtcca aaagcccgcg ctggatgatc gcctacaagt tttccgccga
     5881 gcaggctcag acgcggctcc atgggatcga gctgcaggtg ggccggacgg gggcggtcac
     5941 gccggtggcg cacctggagc cggtgtttgt cgccggttcg acgatcagcc gggcgacgct
     6001 gcacaatgcc gacgagatcg agcgcaagga catccgcgtc ggcgatacgg tgatcatcga
     6061 gaaggggggc gacgtcattc cgaaggtcgt gcgcgtgatc gaatcgctgc gcaccggcca
     6121 ggaggcgccc ttcgtctacc cgacggagtg cccggtgtgc ggcggcggcc tgcatcgtcc
     6181 ggagggtgag gcggtgcatc gctgcatcaa cgcctcctgt ccggcgcagg tcaaggggcg
     6241 catcctgcac tacgcctcgc gcaacgcgat ggatatcgac ggactgggcg acaaaatcgt
     6301 cgaccagctc gtggacggcg gccatgtcga ggatatcgcg gggttgtacg aactgacgcg
     6361 ggagcagctc gcggggctgg agcggatggc ggagaaatcg gccgacaacc tgctgcgcgg
     6421 catcgatgaa agccggcgcc ggccgctggc gaacctgatt tatgcgctcg gcatccggtt
     6481 tgtcggttcg gcgggggcgc ggctgctggt ccgccgctat gcgtcgatcg acgcgctgat
     6541 cgccgccggc gaggaggaac tgacggcgat tgacggggtg ggggaagtgg tggcgcggag
     6601 cgtgcgggag ttcttcgccg aggagaagaa tcggcggctg atcgagcggc ttcgcatgca
     6661 cggcgtcaat ctggagcgtc tgcccgagga ggcgcccccc tcggccgagg cgctcgccgg
     6721 atcgccgttc gcgggcaaga cctgcgtgct gacgggcaag ctggagacga tgagccgcga
     6781 ggaaggggag gagcggatcg cggcgctggg cggcaaggcg gcggggagcg tcagcaaaaa
     6841 gaccgatctg gtgatcgcgg ggcccggcgc gggttccaag ctggaaaaag cacgccagtt
     6901 ggggattgag atcatcgacg aggcggagtt catccgtcgg ttggaagccg ccgcgggcgg
     6961 ctgaggcgct acgggaactt gcgaaggctc aatcggcggc ggagaggccg gcgacgggac
     7021 gccgattgcg cgcgtaaact cgcgtgacgt cgccgccggc gacatggagc gcggcggcgg
     7081 ggtcaagccg ttgagccgca acaggctgac cgacgcgcac ctcaaacccg gccttggaga
     7141 gcacgttgag gactttgcgc aggcggttgc ccgactcgcc cggcgccagg cgcaattcca
     7201 cgatcaccgc ccgcaccagg cccagccggc ggcgggcgtc gcgcagcaga tcggcctcgc
     7261 ccgccggaac acgcagccgg agcaggtgga ccggtccgcg caagtaatgc gagagccggc
     7321 gatgatcggg cgctcggtcg cgcttgcggg cgccggtccg gccgccggtc ggcagaaacg
     7381 gatgaatctc gacgtcgcgc cacagcaggc ggcgggcgtt ctcaaccgcg agcgcgtggc
     7441 gccgggggtc cggttcgaac gcgatgatgc gcgcgttggg gaaccgcgat ttgtcatgat
     7501 agattgccag gccatggccc gcccccacgt cgagcaccac gggggcgtcg gtgtcggcgt
     7561 cgaaatccca ggcgcgggcg atcagcgtgg cgaccagcgg cggccatgga tcctcgtcat
     7621 cgggaatggc caggtcgccc tggcgaagcc gcaatacgcc gtcggcgcgg atgacctcca
     7681 ggagggcgcg ggcggggtcg tcgcccgggc gcagacggga acgcgcctcg gccaggcgct
     7741 cgtcggtgac gcggtcggcg cgggcatggg gccggagcaa ttcccagagg cgatcaaact
     7801 caagctggac ggcgatcggg ctggaaggat cggcgaggag gcggagttgg tccgcgcgcg
     7861 gctgcggcgc gtcggcgccg ggccggtgcg acagcaggcg tggatcgtcg gccatgcgtt
     7921 cctgaacgcg gcggtcggcc aggatgcgct cgacgaccga atcgcgagcg aggagcttct
     7981 gaagcagccg ttcatcgatc ggcaggcggt cgagcagccg gtcgtcgagc acgaggcgtt
     8041 ccatgatgat cagcgtgcag agccggtcgt cgcgcaggat gcgctcgagg aagcgcgggt
     8101 cgccgagcag gcggcgggcc aggatttcgt cgtcggccgc cagatccgcc agcagggggc
     8161 tgccgccgtg ggcttgcagg gcgctggcca gcacgggcag cgtgtcgcgc gcggaaagca
     8221 gacgcgcgac gacgaactcg ctttgaagca gccggcccac gaggcgcggg tcgccggtga
     8281 tgaattgcag gacatcgtca tggcgcagca ggcgaccgag caggatcggg ttttcgcgga
     8341 tgctctcgag cgtggcgggg tgggcgagga tggcgccgag cgcgacggga tgggcgagga
     8401 ggcgggcgat cagccgctcc tcgccgagca gccggtcgag ggcggcgtcg cgctggagga
     8461 cgcgctgcag cagaccgtcg tcgccggcga gccggtccag cagctcgccg tgggcgagca
     8521 gacgtgaaac ggtacgttca tgcgcgagca aggcttcgag cacgtcgggc aggtgaacga
     8581 ttcgctcctg ggtccggggg tcgcgcaata ggcgctcgag gcgcgcggga tgcgagagca
     8641 ggaactcgtg aacgcgcgga tcatcgagga ggttgtccag cgcggcgggc aggtcggcga
     8701 tggcgtcgac cgtttcggcc gatgcgatga cgctgtgaag gaacggcttg agctccagag
     8761 cgcggcgcag gacagcgggc cgcgccaaca gccgatcgag catgaacgaa tgcttgacga
     8821 ggcggtcgag cgcgaggtcc tcggcgagca accggtccag aatctcgaaa ttggcgagga
     8881 cccgctcgtg ggcacgcggc gcggcgacga tccggtcgag cagcgcgcca tcggcgagga
     8941 tgagcgggag catgcgggga tcgcgcagca ggccgcgcag ccactcgtgg cgctggaaga
     9001 tccggtcgcg ggcggtggga tcgtccagca tggcctgaag caggtcgtcg cgtgcggcga
     9061 gccgccggag gagggcgggt tcgctcagcg cgatgtccag ccattgagga tccgagcgaa
     9121 ggtgcgcgag cgcctcggac cggcgcagca gcgcgccgag gaacggccgt tcagggcgga
     9181 ggagcgattg aaggaacgtt tcgtctccgg ccagccgctc gacgaccggc gggtgaagca
     9241 gcagttcggc cacgagggcg tcgtcatcca ggatcagccg gcgggcggcg ggatgcgcga
     9301 gcagtcgccg gaggaggccc gttttgccgg cgagcgcctg gaaggcggtt tcggtggcaa
     9361 gcagccggtc gatcagttcg ggccggcgca gcagggccga ccagcccgcc tcgtgcccga
     9421 acaaccggtc gagcagcgcg ggtttcacgg cgaggcgcgg gaacgccgcc ggcgaatcca
     9481 gaacggcctc gagcagcgcg ggtgacgcga gcacccgcgc gagcgcatcc tcgtcgcgca
     9541 gcagccggcg ccgcagttcc gcatcggcct ggagcagggc gaacgccggc tccgattcaa
     9601 gcagcagcgg gagaagatcg cgcgcggcgc gcgccagcgg ttcgcgggcg gcggggcgcc
     9661 gcagcaacgt gacgaacgtc tgttcatcgc gcaggatggc ccgctgcagg cgctcgtcgc
     9721 gcgagagacg gctgagaagg tcgtcgcgcg cgtgcaaacg cgccagcagg ggaggggagt
     9781 cgagcagggc atcgagcagc tcagcccgat cgccgaggcg gttcacgccc tgcccgcgct
     9841 gaagcatcaa ttcgagcagg ccgcccggac ggaaaatgaa atcaagcgcc ggatcggagg
     9901 agaccagccg ctcgacgaac gcgggcgtgg ccagcgcgcg ggccagcagc cgcggatcgg
     9961 agaggagccg gccgaggaac gcctcccggt caaggatgac attcaggagc gggccgtgcc
    10021 ggaggagacg ttcgaggcgg tcgggctgct cggccaggca ggcaatgaag tcgtcgtgcg
    10081 cgatgaggcg atcgagcagg tcgggatgct gggcaaagcg ggatagaacg gcgtcgtgcg
    10141 tcagcaggcg gtccagtgcg ccgggcaatt gaagcaggcg atccagaacg gcggggtgct
    10201 cgaggaaccg gtcgatccaa tcggactggc cgagcaggcg gtcgaacgtc tcttcccgcg
    10261 cgagcaggcg gtcgagcagt tccggttggc cgacgagggc gcgcagcgat ttctcatcgg
    10321 cgaggatgag gtgcaggagc gcctcgtggg cgaagatccg cgcctggccc tggggcgaag
    10381 tgatgatgcg gctgaggagt tcctcgtttt cgagcagcag cgggagcgcg cggcggtcgc
    10441 ggaggagcag gtgaagcagc cgggtatcgt cggcgatgag cgcggcgagg cggtcatcgg
    10501 cgagcagccg gcgcagcagg tcctcgtgtt cgaggagggc ggccagcgtg cggtcgtcgc
    10561 cgagcaggcg gtgcagcagc agggggtcgg cgagcagctt ttcggcgctg cgcgggtggt
    10621 gcaggatcag gctgagttgg gcgtcgttct cgatcagccg gtggaacgcc tcctcggtgg
    10681 ggaggatgcg gtcgagcagg acccggtcgc gaaggatttg atcgatggcc ggttctgagt
    10741 gcagaatgtc gcgcagcagg tcggggtcgg cgaggcacag gtcgatgacc tgttcgcgga
    10801 tggtcgggtc gcgcagcgcg cccgccagca ggccctgctc gtcgcggagc cgctcgcgca
    10861 ggcgcttgtc gcgccggtcc ttgatgccgc ggatcaccag gttgcctgcc ctcgttcgtc
    10921 aatggaatgt tcagtccgcc ggagcgacaa gcccgtgcaa gaatcgcgag caaaacagga
    10981 tacgaccgtc cgtgccggag cgccactgtc gtatttcctt tgccagagcg gttccgaaga
    11041 acaggtccgg gggaggatcg ccgccgggcg tccgtcaaat cgttgttgtt tgcgagggcg
    11101 tcgagtagga taatttgcac gccggaattg catcacacga aagcaggagc gaacaatcgt
    11161 gtcaagccgt ggatttacag tatactgcgc catccagccc tttctgttgg cgacgctggt
    11221 ctgctcgctg agcggatgta cttcggagaa gccgtcttcg gagtcgggat catccaagcc
    11281 ggacctcctg gtgatggaaa cgcctgttct gccggaggtg gaaccacccg aggtaattgc
    11341 cgagccggag gaggccgaga gcacacccac ggactccctg acgaccggcg cgacgggaga
    11401 tgaggctgaa gcgccagtcg gttttgagcg gaacatccag gattttcccg cgaatctgct
    11461 gcagaatccc tcattcgagg cgtgggaaga ttcactggtc ggatggcagg tgacgggcgc
    11521 cgaggtggcc gc
//
LOCUS       DAOPCO010000116        11476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5541_length_11476_cov_5.289379, whole genome shotgun sequence.
ACCESSION   DAOPCO010000116 DAOPCO010000000
VERSION     DAOPCO010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11476
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5541_length_11476_cov_5.289379"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PLS90_09440"
     CDS             complement(<1..172)
                     /locus_tag="PLS90_09440"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK02668.1"
                     /translation="MANYLVTGGAGFIGSNLVEYLLDAGEAVRVLDDFETGRRENLAP
                     FARRIELIEGSLT"
     gene            complement(182..2449)
                     /locus_tag="PLS90_09445"
     CDS             complement(182..2449)
                     /locus_tag="PLS90_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPK02669.1"
                     /translation="MRPISVLLLAAAGWSLSAAPGLAVNSEAIKDDTYENLGEGQFES
                     VALSSDGFVRPAFAREQLGESGTPIIWDAVTEAGGTVLAATGHEGRLVRILDETTSET
                     VAQVAEPELTSLLRLQDGTVLAAAAPSGHIYRLAKDDTLTTYAAVEAKFIWRMVEGPN
                     GDVFVATGAEGKLFRLRPSGATADVREIHDFKSANLLDLWIDRDGLMGQAGALYIGGQ
                     DPGWLYRYLPEADQVEVVFNPRAEEIRAVRPYARGLALAVNTERAPTPQAMNLTMRMA
                     GAPPAGPAPGAPQMPGDVSQALAEALSPARQPDYGTPRSEVVLLQRDGFSRTLWNAPE
                     RPIHDLAVSPDGEGVLAAAGGNGRLFEVRDDGEYAIVADLRDDYIVRLAESGEQLLMT
                     TARNGTVWAIHDGAAADAAYISRPLDAGALVRWGHLYWHGEIPAGQSVRVMTRLGNDG
                     DPESDLWGAWSEAREVSPGQALELPGGPARFLQYKVQFGKPGRGGESARMDYVEVFYI
                     EPNAAPRITQLTVTPAGGQQPGRPSQPPQRPGQPPQPPQPGGPGGSGMPPGQAQPSAG
                     GVEERSNAMTINIQWQAEDPNEDTLRYHLYYRAADEAEWKLIDDELDVNQLAIGVGGI
                     ADGRYRFKVRATDQFDNPPGEGLLAEEISDEIVIDNTRPEFENLQAEVDGLKATITFR
                     LRDELSLISSVKVDIDNGDSYPLLPVDGMADELSEEYRFVTPALDPGEHVATFNATDR
                     EGNTQVRKIIFNVRR"
     gene            complement(2475..4361)
                     /locus_tag="PLS90_09450"
     CDS             complement(2475..4361)
                     /locus_tag="PLS90_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HPK02670.1"
                     /translation="MPFCLSCRLALLSLLAVAVAGAGAQGAEPLPAAFMPSSEVRPGM
                     RGEGRTVFRGFDVETFQAEILGVDHNAYPGSNMILARLSGPLLEQHGVVAGMSGSPVF
                     IDGRLIGAVAYGWSFAYHPYCGITPIEDMWSVWNKVDEAPATTRMARNGAGRPWNYRE
                     AWDAYQRRLAGEPAEAQPPPTGFRPSLPQFRGLEGEMRPLMTPVFVSSASPRTRDRLG
                     RFLAGRGLELFAAGGLTGADAAAPMDAPPLQAGSSLGVPMITGDLMLGGVGTVTWADG
                     DRIIAFGHPMYALGPTNAPMSPSRVLGFMQSYNRSFKLAEVGPAIGTITQDRLFAVAG
                     RVGPTPGQVEVRAEIVGEAAHRAREFRFKIWDHPDFMPMMVVTALEEAYLSSVSEAGD
                     YTARTVTRIRMRDGREIEKSFVASSDVFTILSASYEMMFDLFLLTENPFRVGQIDRID
                     MRTEVNPGIRRDSMLWMEPEYSRYEPGELVQIRARFQEYLGPEYDRVLELRLPEDLKP
                     GTYVLHVADASTAVRIESMARPGQFLADDFDDLIRIARASHVPEDKLYLYLYEPALGV
                     DLLEHSLGGMPVSVESLIQATAPAGLQSMSVGRLAQTQTYDFPAPVYGAQTLLLDVVE
                     HVQR"
     gene            complement(4585..11436)
                     /locus_tag="PLS90_09455"
     CDS             complement(4585..11436)
                     /locus_tag="PLS90_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK02671.1"
                     /translation="MSVVSNRTMAGALLGLGLWLLAGVAQAGAPFEVWFPVDGNINTT
                     LDDAAVARSVRWPEGLDPQFLYYRRPTGFGDFEVLTRSLNDNELTEFDVREAINRAVE
                     TWNDALDDFQFPPPIAADLWAPHPNSPPWWEYHPRNSALDGYNLITFLDDETFPGDVT
                     GGQIAATLLTFFHRDFSLEDILAGYPEVDPDLLNSLHFGDTDVTRVALDFDGDDQLDL
                     FFPEIRNYKQGELIDVDIIFATTLTYYDWPEDPDDIPVNMEPFVAGSPDIQATMMTQL
                     GLAQGIGLTYIIDSVMYPFTEGPSSPFPTNPYRKRELAFDDIMTARLTHNPNWLDPNF
                     GAIAGSVLEGAVVDGDAIDVDDDGEVTQPVDEENELDAYIVQKPVFLGVRPVQLAESA
                     VLAGGNTGRVDPEYTQHPDNIEAESVLGDANDGTYRMIAQVLTGRELVIQRGEGDDDL
                     FFPIDIEGDEEPDEIEGGITLEDDGVVDTVSRLNSAYLFPGLPSHDDNGTTLSYALYL
                     DEDGEYPLNPVHDFFLEEEDYPPEFYGGPMLTITQGNGQAPVFFNRADREFENEYITV
                     DVDLIGRFALSINDGQALLSGFRNGPESFVSVTSPLGQFSNKFTAIGNVVQSMLISDA
                     DKRATGVFSRNNDFELRITIRETENGGESGINDGVEVTYSLTNPTFTPRVYSLRQVLD
                     TLLFGSENPIYVLDYEGTQEVLDESIALTGDEIPTNVIYQTSIADPVFRGYINLRGAG
                     TVTPDTVIIDRLSALENDGKARGTDLRGPDAGALDTGVALLWEGIEVPGQETVNLSFN
                     IGFLPPGELRDSWVPFPDGDVTIGDLTGFEDDPGRVALVHVTQGEITDNIDIITNTGE
                     EIEGVAQVSPEEGYASGPLRFRRARNGGFPDDDFVTMHGALGDIDNDGDLDCFVANFG
                     GGADLVASRINRLYLNEQRVLPDGTIEYYFRDVTLGEDGIKGTPDDRFDRIVAENSVG
                     VNMADFDGDGDIDLFVTNINGPNRFYENLGPDPDKIGFFADISAEVLPGLLNRGWDEQ
                     DPSGWDFPHRAAVGDIDSDGDLDLIISQRIPFFDDTQTNAWADIAPRDEDFPLGDQLM
                     DSRADLFSEALLFAERVLVNQINQPNYSPYKRGFYFVDETLGSDERAGTLTSLVTQRV
                     TLNSSINEGVALISFDSSELDRMPPVFPIMFNISDDGTINGVASPMAAAGAEPRLGPY
                     VSSASLDLFSVRNLSEDILYVSGLIPLTLDVEEGEGIVTAGLRSALTGLTFDGGHQAY
                     FRNVDMWSMEEGLLGADQVDDGYFACFNYNLDYGVATGTTQFDMLPFDQAAIGEGDPP
                     SPTVMPTAGGLFAIEVDPTNPSRLRHDAFPLLIGFPEGHDGDWNFQNEPPFERDLASN
                     VSRVGWAAYIADFQNRSAPRPFIAADNEDGLGGPPYTSNYTDEFGDYSAHGVARGFGT
                     SEAGGIGGIAVQYLQNYPGIYTGLADSEEVEYYPELAVEPSEGAESVQAGEPFGVAVA
                     DFDWDGDYDFFLANTTVPGITINPSTVIIGGNPAPNQLFLNDGFARFTEMSDALLPNN
                     ELISMHTVSGDLDNDGDIDLVVFNANDGNEIFFNTIFNKPPDLAKTNDATLFYETTRL
                     FLPDLSGSVLAPPAPIDSSFSGLSIRTTVGDINHDGRPDMVIAEGGQYSADGDFTRVL
                     LNKGQPIGDSVPVFKPGDATFPAPHVDMWNHNFLADPLPLVGFMGEQFETSDVALGDV
                     DNDGDLDMFIASVGSGTRLYLNQDSDIQLFNSAPDDDALGDAIFDPGPGFPVLEDPDG
                     GDLLGVVLKQQNRRIKLADLNGDGLLDVVIANGLTNSGAPNALLLNQPGDPGTFIDVT
                     ETNLPTTEIGGVTMGIPDNTNDVGLADFDADGDIDILFVNESTAGAPYGFRLLLNDGN
                     AMFEETEPGSLIPEFIGLKPYAIVLADFDNLGETTEDVNFNGVLDPGEDTNGNGILDW
                     EDKGESDGICNASVDIFISVMNGPDLLLVNDPAQPGHFLAGESSSRLPDTEFGGGESR
                     GCDAGDIDLDGDMDIVVAQYVGGVTRHVRVWRNGTRTFADGTVRQGYFTEISHEVPFS
                     RANLHVSEIGDEPDDAFDFAGWATDVDLFDADLDGDLDMMITCLGRTGELAVMGAQDI
                     FYCNRLIGDGFNAKPEGIIQTPGDPIVFSATPPAAELGETKQVRLIGGNFAPDQVTYL
                     GGEGEDAQYERQPGVHVSFGQGIEVLEVDRKSGAVLDVVIRVKEDATLGPRRIAVQNM
                     TSGGSTTTKMGIFTVYEELPDAIRNAVPDTVWTMY"
BASE COUNT         1922 a         3947 c         3593 g         2014 t
ORIGIN      
        1 cggtcaggct cccttcgatc agctcgatgc gccgagcgaa tggcgccaga ttttcccgcc
       61 ggcccgtctc aaaatcatcc agcacgcgca ccgcctcgcc ggcgtcgagc agatactcca
      121 ccaggttgga gccgatgaac ccggcgcccc ccgtgaccag gtaattcgcc atagagccgt
      181 cctaccttcg gacgttgaag atgatcttgc gcacctgcgt gttgccttcg cgatcggtgg
      241 cgttgaacgt cgccacgtgc tcgcccgggt cgagcgccgg cgtcacgaag cgatactcct
      301 cgctcaattc atcggccatg ccgtcgacgg gcaacagcgg ataactgtcg ccgttgtcga
      361 tgtccacctt gaccgacgag atcaacgaca actcatcgcg caaccggaag gtgatggtcg
      421 ccttcagacc gtccacctcc gcctggaggt tttcaaattc cggccgcgta ttgtcgatca
      481 caatctcatc ggagatttcc tcggccagca gcccctcgcc cggcgggttg tcgaactgat
      541 ccgtcgcgcg caccttaaac cggtagcgtc cgtcggcgat cccgccgacg ccgatcgcaa
      601 gctggttcac gtccagttcg tcatcgatca gcttccactc cgcttcgtcc gcggcccggt
      661 agtagaggtg gtagcgcagc gtgtcctcgt tgggatcttc ggcctgccat tggatgttga
      721 tcgtcatggc gttggagcgt tcctcgacgc cccccgccga gggttgcgcc tggcccggcg
      781 gcatccccga accgccgggg ccgcccggct gcggcggttg cggcggctgg cccggtcgct
      841 gcggcggttg cgacggacgc cccggttgct gcccccccgc cggcgtgacg gtcaactgtg
      901 tgatccgcgg cgcggcgttc ggctcgatgt agaacacctc gacgtaatcc atccgggccg
      961 actcaccgcc gcgccccggc ttcccgaatt gaaccttgta ctggaggaac cgcgccggcc
     1021 cgcccggcag ctcgagcgcc tggcccggcg atacctcgcg agcctccgac catgcgcccc
     1081 acagatccga ttccgggtcg ccgtcgttgc ccagccgcgt catgacgcgc accgactggc
     1141 cggcggggat ctcgccatgc cagtagaggt gcccccagcg caccagcgca ccggcgtcga
     1201 gcggccgcga aatgtaggcc gcgtcggcgg cggcgccgtc atgaatcgcc cagacggtcc
     1261 cgttgcgcgc cgtcgtcatc agcaactgct cgccgctctc ggccagccgc acgatgtaat
     1321 cgtcgcgcag gtcggccacg atcgcatact cgccgtcatc gcgcacctcg aagagccgcc
     1381 cgttgccgcc cgccgccgcc agcacgccct cgccatcggg cgagacggcc aggtcgtgga
     1441 tcggccgctc cggcgcgttc cacagcgtcc ggctgaatcc gtcgcgctgc agcaggacga
     1501 cctcgctgcg cggcgtcccg taatcgggct ggcgggccgg cgagagcgcc tcggccagcg
     1561 cctggctcac gtcgcccggc atctgcggcg cgccgggagc cggccccgca gggggcgccc
     1621 ccgccatccg catcgtcagg ttcatcgcct gcggcgtcgg cgcccgctcg gtattgaccg
     1681 ccagcgccag accccgcgca tagggccgca ccgcgcggat ctcctccgcc cgcgggttaa
     1741 acaccacctc gacctggtcc gcttccggca gatagcggta gagccagccc ggatcctggc
     1801 cgccgatgta gagcgcgccg gcctggccca tcaacccgtc gcggtcgatc cacaggtcga
     1861 gcaggttggc cgatttgaag tcatgaattt cccgcacgtc ggccgtcgcg ccgctcggcc
     1921 gcaggcggaa cagtttgccc tcggcccccg tcgcgacaaa cacgtcgccg ttgggcccct
     1981 cgaccatccg ccagatgaac ttggcctcga ccgccgcgta ggtcgtcagc gtgtcgtctt
     2041 tcgccagccg ataaatgtgg cccgacgggg cggccgccgc cagcaccgtc ccatcctgaa
     2101 gccgcagcag cgacgtcagt tccggttcgg ccacctgcgc gaccgtttcg ctggtggtct
     2161 cgtccaggat gcgcaccagc cgcccttcgt ggccggtcgc ggccagcacc gtcccgccgg
     2221 cctcggtcac cgcgtcccag atgatcggcg tcccgctttc accaagctgt tcgcgcgcga
     2281 acgccggccg gacgaacccg tccgaactga gcgccaccga ttcgaactgg ccctcgccca
     2341 gattttcata ggtgtcgtcc ttgatcgcct cggaattgac ggcgaggccc ggggcggccg
     2401 acaggctcca tccggccgcc gccagcagca gcacgctgat cggtcgcaag agcgcttcct
     2461 tgactgatcc ggtcttaacg ttggacatgt tccaccacat cgagcaacag ggtctgcgct
     2521 ccgtataccg gggcggggaa atcgtaggtc tgcgtctgcg ccagccgccc cacgctcatc
     2581 gactggagcc cggccggggc cgtcgcctga atcaggcttt cgaccgacac cggcatcccc
     2641 cccagcgaat gctccagcag atcgacgccc agcgccggct cgtagagata gagatacagc
     2701 ttgtcctccg gcacatgcga ggcgcgcgcg atgcggatca gatcgtcaaa gtcatcggcg
     2761 aggaactgcc ccggccgcgc catcgattcg atccgcaccg cggtggaggc gtcggccacg
     2821 tgcagcacgt aggtgccggg tttcagatcc tccggcagcc gcagctccag cacccggtcg
     2881 tattccggcc ccaggtattc ctggaaccgg gcgcggatct ggaccagctc gcccggttca
     2941 tagcggctgt actcgggctc catccacaac atcgaatcgc gccggatgcc cggattgact
     3001 tccgtccgca tgtcgatccg gtcgatctgc ccgacccgga acgggttttc ggtcagcaga
     3061 aacagatcga acatcatctc atagctggcg ctgagaattg taaacacatc ggacgaggcg
     3121 acgaacgatt tttcgatctc gcggccgtcg cgcatgcgga tccgcgtcac ggtccgcgcc
     3181 gtgtagtccc cggcctccga gaccgacgaa agatacgctt cctccagcgc cgtcacgacc
     3241 atcatcggca tgaaatcggg gtgatcccag atcttgaacc ggaactcgcg cgcgcgatgc
     3301 gccgcctcgc ccacgatctc ggcgcgaacc tccacctgcc ccggcgtggg ccccacgcgc
     3361 cccgcgacgg cgaacagccg gtcctgcgtg atcgtgccga tggcggggcc gacttcggcc
     3421 agcttgaatg atcggttgta gctctgcatg aaccccagca cgcgcgaggg cgacatcggc
     3481 gcattcgtcg gcccgagggc atacatcgga tggccgaacg cgataatccg gtcgccgtcc
     3541 gcccaggtga cggtgccgac gccccccagc atcaggtcgc cggtgatcat cggcacgccc
     3601 agcgacgagc ccgcctggag cggtggggcg tccatgggcg ccgcggcgtc ggcgccggtc
     3661 aggccgccgg cggcaaacag ctccaatccg cgcccggcga ggaagcgccc cagccggtcg
     3721 cgcgtccgcg gcgaggccga cgacacgaag accggcgtca tcagcggccg catctccccc
     3781 tcaagcccgc gaaactgcgg caacgagggc cgaaatcccg tcggcggcgg ctgcgcttcg
     3841 gccggctcgc ccgccagccg ccgctgatag gcgtcccatg cctcgcggta gttccacggc
     3901 cggcccgcgc cgttgcgcgc catccgcgtt gtcgccggcg cctcatccac cttgttccag
     3961 accgaccaca tgtcctcgat cggcgtgatg ccgcagtacg ggtgataggc gaacgaccag
     4021 ccgtaggcga cggcgccgat cagccggccg tcgatgaaca cggggctgcc gctcatcccg
     4081 gccacgacgc catgctgctc gagcagcggg cccgacagcc gcgccaggat catgttgctc
     4141 cccggatagg cgttgtgatc gacgccgagg atttcggcct gaaacgtctc gacgtcaaat
     4201 ccgcgaaaga ccgtccgccc ctcgccgcgc atccccgggc gcacctcgct cgacggcatg
     4261 aacgcggccg gcagcggctc ggctccttgc gcaccggcgc cggcaaccgc gacggccagc
     4321 agcgagagaa gcgccaggcg acacgacaaa cagaaaggca tgaaggggga ccttggggcg
     4381 gagatgggcg aggcccggtc gcactgacgc gcatccgggc gggactgggt atccgcaaaa
     4441 ttctaccggc ttggcccggc cagtgcaatt ttcaaaagcc tgcgtgccgt ttcgtgtctt
     4501 gcgtgatccc ttccctgacc caaaaacaaa cgcggggaaa accgcgctgc cggctttccc
     4561 cgcgctcatt ccattcaatc gcgatcaata catcgtccag actgtgtcgg gcaccgcgtt
     4621 gcggatcgcg tcgggcagct cctcatagac cgtgaagatg cccatcttcg tcgtcgtcga
     4681 gccgccgctg gtcatgttct ggacggcaat ccggcgcggc ccgagcgtcg cgtcctcctt
     4741 gacgcggatc accacgtcga gcaccgcgcc actcttgcga tcgacttcca gcacttcgat
     4801 cccctggccg aacgagacat gcacgccggg ctgccgctcg tattgcgcgt cctctccctc
     4861 gccgcccagg taggtcacct ggtcgggagc gaagttgccg ccgatcagtc gcacctgctt
     4921 cgtctcgccc agttcggccg ccgggggcgt ggcgctgaac acgatcggat cgcccggcgt
     4981 ctggatgatc ccctccggct tcgcgttgaa cccgtcgccg atcagccggt tgcagtagaa
     5041 gatgtcctgc gcgcccatta cggccagctc gccggtccgg ccgaggcagg tgatcatcat
     5101 gtccaggtcg ccgtccaggt cggcgtcgaa cagatcgacg tcggtcgccc atccggcaaa
     5161 atcgaacgcg tcatcgggct cgtcgccgat ctccgagacg tgaagattgg cgcggctgaa
     5221 cgggacttca tgcgagatct cggtgaagta gccctggcgg accgtcccgt cggcaaaggt
     5281 gcgcgtcccg ttgcgccaga cgcggacgtg ccgggtcacg ccgccgacat actgcgccac
     5341 gacgatgtcc atgtcgccgt ccaggtcgat gtcgccggcg tcgcaaccgc gcgactcgcc
     5401 gcccccgaac tcggtgtcgg gcaggcgcga actggactcc ccggccagga aatggcccgg
     5461 ctgcgccgga tcattcacca gcagcagatc gggtccattc atcacgctga tgaagatgtc
     5521 gaccgacgca ttgcagatcc cgtccgattc gcccttgtcc tcccagtcca ggatgccgtt
     5581 gccgttggtg tcttcgcccg ggtcgagcac gccgttgaag ttcacgtcct cggtcgtttc
     5641 acccagattg tcgaagtcgg ccagcacgat ggcatacggc ttgagtccga tgaactccgg
     5701 aatcagcgac cccggttcgg tctcctcgaa catcgcgttg ccgtcgttga gcagcaggcg
     5761 gaagccgtac ggcgcgccgg ccgtcgactc gttgacgaac agaatgtcga tgtcgccgtc
     5821 ggcgtcgaaa tccgccagcc ccacgtcgtt ggtattgtcc gggatgccca tcgtgacgcc
     5881 gccgatctcc gtcgtcggca ggttggtctc ggtcacgtcg ataaaggttc cggggtcgcc
     5941 cggctgattc agcagcaggg cgttgggcgc gccggaattc gtcagcccgt tggcgatcac
     6001 gacgtccagc aggccgtccc cattcaggtc ggccagcttg atccgtcggt tctgctgttt
     6061 aagcacaacg ccgagcaggt cgcccccgtc gggatcctcc agcacgggga aacccggccc
     6121 ggggtcgaag atcgcgtcgc ccagggcgtc gtcgtcgggc gcggagttga acaactgaat
     6181 gtcgctgtcc tggttcagat agagccgcgt gccgctgccg accgaggcaa taaacatgtc
     6241 gaggtcgccg tcgttgtcga catcgcccag cgccacgtcg ctcgtctcga actgctcgcc
     6301 catgaacccg accagcggca gcgggtcggc caggaaattg tggttccaca tgtccacgtg
     6361 cggggccggg aaagtcgcgt cgcccggttt gaacaccggc accgaatcgc cgatcggctg
     6421 gcccttgttc agcagcacgc gggtgaagtc gccgtcggcc gagtactgac cgccttcggc
     6481 gatcaccatg tcgggccgcc cgtcgtggtt gatgtcgccc accgtcgtcc ggatcgaaag
     6541 cccggagaaa ctcgagtcga tgggcgccgg cggcgccagc acgctgccgc tcagatccgg
     6601 caggaacaac cgcgttgttt catagaacag tgtcgcgtcg ttcgttttcg cgaggtccgg
     6661 cggcttgttg aagatcgtat tgaaaaagat ctcgttcccg tcgttggcgt tgaacacgac
     6721 caggtcgata tccccgtcat tatccaggtc gcctgacacc gtatgcatac tgatgagctc
     6781 attgttgggc agcagcgcat cggacatttc ggtgaaccgg gcgaaaccgt cattcaggaa
     6841 cagctgattc ggcgcgggat tgcccccgat gatgaccgtg ctgggattga tggtgatgcc
     6901 ggggaccgtc gtattggcca ggaagaagtc ataatcgccg tcccagtcaa agtccgcgac
     6961 ggcgacgccg aacggctcgc ccgcctggac cgactcggcc ccttcggacg gttccacggc
     7021 gagttccgga tagtactcca cttcctcgct gtccgctaat ccggtgtaga tgccgggata
     7081 gttctgcaga tactgcacgg caatcccacc gatgccgcct gcctcggagg tgccaaatcc
     7141 gcgcgcgacg ccatgggccg aataatcccc aaattcgtcg gtatagttgg acgtataagg
     7201 cggcccgccc agaccatcct cattatcggc ggcgatgaac ggccgcggcg ccgaacggtt
     7261 ctggaaatcg gcaatatacg ccgcccagcc cacgcgcgag acgttgctgg caaggtcccg
     7321 ttcgaacggc ggttcattct ggaagttcca atcgccgtca tgcccttcgg ggaaaccgat
     7381 cagcagcggg aacgcgtcat gacgcaaccg cgacggattg gtcggatcga cctcgatcgc
     7441 gaacaggccg ccggccgtcg gcatcaccgt cggcgacggc ggatcgcctt cgccgatcgc
     7501 cgcctgatcg aacggcagca tgtcgaactg ggtggtcccg gtcgccactc cgtagtccag
     7561 gttgtagttg aagcaggcga aatagccgtc gtcgacttgg tcggcgccga gcaacccctc
     7621 ctccatcgac cacatgtcga cattgcggaa atacgcctgg tggccgccgt cgaatgtgag
     7681 gcccgttaag gccgagcgga gccccgccgt cacgatgccc tcgccttcct cgacatccag
     7741 cgtcaaggga atcagaccgc tcacatagag aatgtcttcc gacaggtttc gcaccgagaa
     7801 cagatccaga ctggccgacg agacatacgg tcccagtcgc ggctcggctc cggcggccgc
     7861 catcggcgag gcgacgccgt tgatcgtgcc gtcatcggaa atattgaaca tgatcgggaa
     7921 gacgggcggc atccggtcca gttccgagga gtcgaacgag atcagcgcca ccccttcgtt
     7981 gatgctgctg ttcagcgtca cccgctgggt cacgagcgag gtcagcgtgc cggcccgttc
     8041 atcgctgccc agcgtttcat ccacgaagta gaagccgcgc ttgtaggggc tgtaattcgg
     8101 ctgattgatc tggttgacaa ggacccgttc ggcaaacagc agcgcctcgg agaagaggtc
     8161 cgcgcgcgag tccatcagtt gatcgcccag cgggaaatcc tcgtcgcgcg gtgcgatatc
     8221 ggcccacgcg ttggtctgcg tgtcgtcgaa gaacgggatc cgctgactga tgatgaggtc
     8281 cagatcgccg tcgctgtcga tgtcgccgac cgcggcgcga tgcggaaaat cccagcccga
     8341 cgggtcctgt tcgtcccagc cgcgattgag caggccggga aggacttccg ccgaaatgtc
     8401 ggcgaagaac ccgatcttgt ccggatcggg gcccagattc tcgtagaacc ggttcggccc
     8461 gttaatgttc gtgacgaaca gatcgatgtc gccgtcgccg tcgaagtcgg ccatattcac
     8521 gccgacgctg ttctccgcca cgatccggtc gaagcggtcg tcgggcgtcc ccttgatccc
     8581 gtcctcgccc agcgtcacgt cgcggaagta gtactcgatc gtgccgtcgg gcaggacccg
     8641 ctgttcgttc aaatacagcc ggttgatccg gctggcgacc aggtcggcgc cgccgccgaa
     8701 attggccacg aagcagtcca ggtcgccgtc gttgtcgatg tcgcccaacg ccccgtgcat
     8761 cgtgacgaag tcatcgtccg ggaacccgcc gttgcgcgcc cggcggaacc gcagcggacc
     8821 ggaggcgtag ccttcctcgg gcgaaacctg cgccacccct tcgatctcct cgccggtgtt
     8881 ggtgatgatg tcgatattgt cggtgatctc gccctgggtg acatggacga gcgcgacgcg
     8941 ccccggatcg tcctcgaacc cggtcagatc gccgatggtc acgtcgccgt cggggaacgg
     9001 cacccacgaa tcgcgcagct cgccgggcgg caggaagccg atgttgaacg agaggttcac
     9061 ggtttcctga ccgggcacct cgatcccttc ccacagcagc gccacgccgg tgtcgagggc
     9121 gccggcgtcc ggcccgcgca gatccgtgcc gcgcgccttc ccgtcgttct ccagggcgct
     9181 cagccggtcg atgatcaccg tgtcgggcgt gacggtcccc gccccgcgca gattgatgta
     9241 gccgcggaaa accgggtcgg cgatcgatgt ctggtaaatc acgttggtcg gaatctcatc
     9301 gcccgtcagc gcgattgatt cgtccaggac ttcctgggtc ccctcatagt cgagcacata
     9361 gatcgggttc tcgctgccga acagcagcgt gtcgagcacc tggcgcagcg agtagacgcg
     9421 cggggtgaac gtcgggttcg tcaggctgta ggtcacttcc acgccgtcat tgatgccgct
     9481 ctcgccgccg ttctcggtct cgcggatcgt gatgcgcagt tcaaaatcgt tgttgcggct
     9541 gaagacgccc gtcgcgcgct tgtccgcgtc cgaaatcagc atcgactgaa cgacgttgcc
     9601 gatcgcggtg aacttgttgg agaactggcc cagcggcgag gtgaccgaca cgaacgactc
     9661 gggcccattg cggaagcccg acagcagcgc ctggccgtcg ttgatcgaca gcgcgaaccg
     9721 cccgatcaga tcgacgtcga ccgtgatgta ctcgttctcg aactcgcggt cggcgcggtt
     9781 gaagaacacc ggcgcctggc cgttgccctg cgtgatggtc agcatcggtc cgccgtaaaa
     9841 ctccggcgga tagtcctctt cctccaggaa aaaatcatgc accgggttga gcgggtattc
     9901 cccgtcctca tccagataga gcgcgtagct gagcgtcgtg ccgttgtcgt cgtgcgaggg
     9961 cagaccgggg aacaggtagg ccgaattgag ccgcgacacg gtgtcgacca ccccgtcgtc
    10021 ctccagcgtg atgcccccct cgatctcgtc gggctcctcg tcgccctcga tgtcgatcgg
    10081 aaagaacagg tcgtcgtccc cctcgccgcg ctggatcacc agctcgcgcc cggtcaaaac
    10141 ctgggcgatc atccggtagg tgccgtcgtt ggcgtcgccc agcacggatt cggcctcgat
    10201 attgtcgggg tgttgcgtgt actccggatc gacgcggccg gtgttccccc ccgccagcac
    10261 ggcactctcg gcaagctgca ccggccgcac gcccaggaag accggcttct ggacgatgta
    10321 ggcgtccagt tcgttctcct cgtcgaccgg ctgcgtcacc tcgccgtcgt catcgacatc
    10381 aatcgcgtcg ccgtcgacga ccgcgccctc cagcacgctg cccgcgatcg ccccgaaatt
    10441 ggggtccagc cagttggggt tgtgcgtcag tcgcgccgtc atgatgtcgt cgaacgccag
    10501 ctcgcgcttg cgatacgggt tcgtcgggaa cggactgctt ggcccctcgg tgaacggata
    10561 catcaccgaa tcgatgatgt aggtcaggcc gatcccctgg gccagtccca gttgcgtcat
    10621 catcgtggcc tggatatcgg gcgagccggc cacgaacggc tccatattca cggggatgtc
    10681 gtccgggtct tcgggccagt cgtagtaggt cagcgtcgtg gcgaaaatga tgtcgacgtc
    10741 gatcagttcg ccctgcttgt agttgcggat ctcgggaaag aacaggtcca gttggtcgtc
    10801 tccatcgaaa tccagcgcca cccgcgtcac gtcggtgtcg ccgaagtgca gcgaattgag
    10861 caggtcgggg tccacttcgg gatagcccgc caggatatcc tccaggctga aatcgcgatg
    10921 gaagaaggtc aacagcgtgg cggcgatctg gccccccgtc acgtcgccgg gaaacgtctc
    10981 gtcatccaga aaggtgatca ggttgtagcc gtcgagcgcg gaattgcgcg gatgatattc
    11041 ccaccagggg ggcgagttgg ggtgaggcgc ccacaggtcc gcggcgatcg gcggcgggaa
    11101 ctggaagtcg tccagcgcgt cgttccaggt ttccacggcg cggttgatcg cctcgcgcac
    11161 gtcaaattcg gtcagttcgt tgtcgttcag gctgcgcgtc aggacttcga aatcgccgaa
    11221 tcccgtcggc cgccgataat acagaaactg cggatccagc ccctcgggcc agcgcaccga
    11281 ccgggcgacg gcggcgtcgt ccagcgttgt gttgatgttg ccgtcgaccg ggaaccagac
    11341 ctcgaacggc gcgccggcct gcgccacgcc cgccagcagc cacaagccca gccccaacag
    11401 ggccccggcc atcgtgcgat ttgagacaac tgacaccatc aatctccctt gcctgtcacc
    11461 ccggcctgcc gatggg
//
LOCUS       DAOPCO010000117        11366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5610_length_11366_cov_4.435594, whole genome shotgun sequence.
ACCESSION   DAOPCO010000117 DAOPCO010000000
VERSION     DAOPCO010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5610_length_11366_cov_4.435594"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..353
                     /gene="secE"
                     /locus_tag="PLS90_09460"
     CDS             93..353
                     /gene="secE"
                     /locus_tag="PLS90_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPK02672.1"
                     /translation="MGKIIVLVVLALIVLAVLVSLPKLMPKLRSLRKFFDEVALEMTK
                     VTWPTQQEVINSTALVLVVTVILTVVVGFADLIFGRLVRLLF"
     gene            448..984
                     /gene="nusG"
                     /locus_tag="PLS90_09465"
     CDS             448..984
                     /gene="nusG"
                     /locus_tag="PLS90_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPK02673.1"
                     /translation="MSGTAWYAVHTYSSFENKVKQTIERMAQLEGLSDKVHRVIIPLE
                     PVIEIKSGKKNTVMRNLMPGYLLVEIEPEDEVFDMIRNIKGVSGFVGGGAKPAPLSQS
                     EVDNILSMLESKADKPKPEVTYQKGDQVKVNEGPFTNFVGTVDHVDQEKHKLQVMVSI
                     FGRPTPVELDVLQVEPAS"
     gene            1101..1526
                     /gene="rplK"
                     /locus_tag="PLS90_09470"
     CDS             1101..1526
                     /gene="rplK"
                     /locus_tag="PLS90_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPK02674.1"
                     /translation="MAKKVIGMVKIQVPAGQANPAPPIGPALGQHGVNIMEFCKQFNA
                     RTKGQEGLIIPAVITVYADRSFTFITKSPPAAVLIKRAAGLAKGSGEPNKNKVGTIQR
                     EQLREIAQIKMNDLNAGSLEAAIDMMAGTARSMGVTVEG"
     gene            1640..2320
                     /gene="rplA"
                     /locus_tag="PLS90_09475"
     CDS             1640..2320
                     /gene="rplA"
                     /locus_tag="PLS90_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPK02675.1"
                     /translation="MPKHGKQYRAAIEQVDRQKRYTISEAVALLKKANFAKFDESVDL
                     DLRLGVDPRNAEQQVRGTVNLPHGTGKKVRVAVFAKGDAAREAEEAGADVVGAEDLVQ
                     KIQGGFLEFDAAVATPDMMREVSKIGRVLGPRGLMPNPKTGTVTNNVSQVVQELKAGK
                     VEYRLDRNANVHCTVGKKSFAEEALAENVQTLIDAIQRARPASAKGVFMRSATLSTTM
                     GPGIKLAV"
     gene            2638..3177
                     /gene="rplJ"
                     /locus_tag="PLS90_09480"
     CDS             2638..3177
                     /gene="rplJ"
                     /locus_tag="PLS90_09480"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPK02676.1"
                     /translation="MLRRPEKVELVNELSEKLRQAQSIILTDFQGMNVAEVSDLRSQL
                     RSQAIEMRVVKNRLIRRALSDAGCDSLDSLLVGNTAVVFGMSDPVAPAKILVKYAKDN
                     EKLKVKGGLLERKALDSAGVDNLSRMPGRHELLTMMARDLKAPATKMATVFQAGLLKV
                     AHAMNALARKKEEAGEAAA"
     gene            3246..3647
                     /gene="rplL"
                     /locus_tag="PLS90_09485"
     CDS             3246..3647
                     /gene="rplL"
                     /locus_tag="PLS90_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002854784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPK02677.1"
                     /translation="MAETLTPDQVLEAVEQWSLLQVNEFVKKFEEKFDVSAAAPVAVA
                     AGPVAGGGGEAAPAAEEKTEFTVVLKAVPADKKIKVIKEVRALKPDLGLKEAKDLVDA
                     APKELLTDVSKDDAEKAKKQLEEAGAEVELK"
     gene            3856..7776
                     /gene="rpoB"
                     /locus_tag="PLS90_09490"
                     /pseudo
     CDS             3856..7776
                     /gene="rpoB"
                     /locus_tag="PLS90_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            8078..>9857
                     /locus_tag="PLS90_09495"
     CDS             8078..>9857
                     /locus_tag="PLS90_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK02678.1"
                     /translation="MATAGITGSQRTMFQPGAPTGRSERIDETNAICRISLASPQQIL
                     KWSSRQRRQAEHLSNLQLGRLGDEIDPTSFGEVKKPETINYRTFKPEKDGLFCERIFG
                     PVKDWECACGKYKRIKYKGIVCDRCGVEVTESKVRRHRMGHIKLAAPVCHIWFYKGTS
                     SRIANLLDISARDLGKVLYFQDYIVVEPGDTPLKEKQILSEEEARKYSEVYGDKVKIM
                     IGAEAVRELLKKINIEEEANRLMLEMRQSDSLQKRTKIIKRLKVVEAFRKSDNRPEWM
                     ILEVLPVIPPDLRPLVHLDGGRFATSDLNDLYRRVINRNNRLKRLIELHAPDVILRNE
                     KRMLQEAVDALFDNSRRSRPTKGHNNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRS
                     VIVVGPDLKFNQCGLPKKMALELFDPFIIHELQKRGHCNTIKSAKRMIEKEEPVVYDI
                     LDDIIQDHPVLLNRAPTLHRLGVQAFMPKLVEGKAIQLHPLACAAFNADFDGDQMAVH
                     VPLSTEAKLEARLLMLAENNILSPANGFPIASPSQDMVLGCFYVTKMVPGAKGEGMRF
                     ADPDEALMAYQYERVALQAEVEVRIDG"
     assembly_gap    9858..9867
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9868..>11366
                     /locus_tag="PLS90_09500"
     CDS             <9868..>11366
                     /locus_tag="PLS90_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK02679.1"
                     /translation="TTPGRIIFSRALPNEIRYFDEDSPYFNKVMIKKTLVQLVDDAHR
                     RCGARRTAQMLDAIMQLGFNFAKRGGMSICVDDMLIPESKPKIIREAVKNVARVQKRY
                     EQGSITNEERYFDVINIWSHASEAVAKDMTLGLEKDQSGLNPIYIMAHSGARGNMAQI
                     RQLAGMRGLMQKPTKKLTGGVGEIIESPIMSNFREGLTVLEYFISTHGARKGLADTAL
                     KTSDAGYLTRRLVDIAQDLIVTEEDCGTDDGIEVEPIKEITAEGERILEPLRDRIVGR
                     VAVRTMKNPVTNKVVVHRNQLIDEQTARDIENAGFERILIRSVLTCESRRGVCAKCYG
                     RNLATGQDVEIGEAVGVIAAQSIGEPGTQLTLRTFHIGGTASLQLEGWYEAVEDGIVH
                     YREGLRTVPREEETGEIVNVVIVRTGTVTVEDKNGNEIQVLPNIPYGATITKAEGQEV
                     KKGEKFVQWDTNQTPIIAESDGIVKFSDIIRGVTVAEDIDSRTGNVQLV"
BASE COUNT         2352 a         3099 c         4183 g         1722 t
ORIGIN      
        1 gggttcgagc ccctcctggc ctgccagcgc agacggtgtg tggatggcga acccgtccgc
       61 aacggcgcaa ttccggggaa gccgcaggat ccatgggtaa aatcatcgtg ttggtggtgc
      121 tggcgctgat cgtgctggcg gtgctggtca gtctgcccaa gctgatgccg aagctgcgca
      181 gtctgcggaa gtttttcgac gaggtggccc ttgagatgac gaaagttacc tggccgacgc
      241 agcaggaagt gatcaactcg acggcgctgg tgctggtggt gacggtgatt ctgacggtgg
      301 tggtcggttt tgccgatctg attttcgggc ggctggtgcg actgctgttc tagcagtgcc
      361 gggccgcgca atcccagaac aatcctagat cagtagaacg gtggtagttg tcccgtggtg
      421 atggtgaggt taggtgagga ggcaacggtg tctggaacag cgtggtatgc ggtacatacc
      481 tactccagct tcgagaacaa ggtcaagcag acgatcgagc gaatggcgca gctcgagggg
      541 ctgagtgaca aggtgcatcg cgtgatcatt ccgctcgagc cggtgatcga gatcaagagc
      601 gggaagaaga acacggtgat gcggaacctg atgccgggct atctgctggt ggagatcgag
      661 cccgaggatg aagtgttcga catgatccgc aacatcaagg gggtgtcggg tttcgtcggc
      721 gggggggcca agccggcgcc gctgtcgcag tcggaggtcg acaacattct gagcatgctc
      781 gaatcgaagg cggacaagcc gaagcccgag gtgacgtatc agaaaggcga tcaggtcaag
      841 gtcaacgagg gaccgttcac gaatttcgtg gggacggtgg atcacgttga tcaggagaag
      901 cacaagctgc aggtgatggt gtcgatcttt ggccggccga cgccggtcga actggacgtg
      961 ctgcaggtgg agccggcgag ctgaggctgg gggggcgcgg cgcctcggga tcggcgggtt
     1021 cgagagccga ggacgagggg cgggagacgg ttttttgtgt gagacgatag acgaattacg
     1081 agaaagatga gtgacggacg atggcgaaga aagtgatcgg catggtgaag attcaggtgc
     1141 cggccgggca ggcgaacccc gcgccgccga tcggcccggc gctgggtcag catggcgtga
     1201 acatcatgga gttctgcaag cagttcaatg cgcggacgaa gggtcaggag gggctgatca
     1261 tcccggcggt gatcacggtg tatgccgatc gcagcttcac gttcatcacg aaatcgccgc
     1321 cggcggcggt gctgatcaag cgcgcggcgg ggctggccaa gggatcgggc gagccgaaca
     1381 agaacaaggt ggggacgatc cagcgcgagc agttgcgcga gatcgcccag atcaagatga
     1441 acgacctgaa cgccggcagc ctggaggcgg ccatcgacat gatggcgggg acggcgcgct
     1501 cgatgggcgt gaccgtggaa ggctgagcgg cccgcagttt cgcccggagg ggaaaagctc
     1561 cgggagtgaa cccaccaatc caacacgtcc cgtgggaggc cgccgggcgc ggccgccagg
     1621 accacaggag aacctcccca tgcccaaaca tggcaagcaa taccgcgcgg cgatcgagca
     1681 ggtcgatcgc cagaagcgtt atacgatcag cgaggcggtg gccctgctga agaaggcgaa
     1741 cttcgccaaa ttcgacgaat cggtcgatct ggacctgcgt ctgggagtcg atccgcgcaa
     1801 tgccgagcag caggtgcgcg ggacggtgaa tctgccgcat gggacgggca agaaagtccg
     1861 cgtggcggtg ttcgcgaagg gggatgcggc gcgcgaggcg gaagaggccg gggccgacgt
     1921 cgtcggcgcc gaggatctgg tgcagaagat ccagggcggg ttcctggagt tcgacgcggc
     1981 ggtggcgacg cccgacatga tgcgggaggt cagcaagatc ggccgcgtgc tgggcccgcg
     2041 cgggttgatg cccaacccca agacggggac ggtgacgaac aacgtgagcc aggtcgtgca
     2101 ggaactgaag gcgggcaagg tggagtatcg gctcgaccgc aacgccaatg tccactgcac
     2161 ggtgggcaag aagagctttg cggaagaggc gctggcggag aacgtgcaga cgctgatcga
     2221 cgcgatccag cgggcgcggc cggcgtcggc caagggggtg ttcatgcgtt cggcgacgct
     2281 cagcacgacg atggggccgg gcatcaagct ggcggtctga gcgcgcaaag tttattcggg
     2341 cgggccccag acggagcgcc gcgcccgaca tcgaccgaga ccgaagacag caggtcccgc
     2401 cccggaggcg ggttaaggaa gcgagggctt cgcctgccga ggttggggtg acaggcccat
     2461 tgaggggcgg ggagcccgcc acagtgcctt gcggggcatg tggtttcccc agccggaacc
     2521 gccgccgcgg ggttccgggt tcctcctgga tggaccgccg aaccgctcca gccccgcccc
     2581 gcggactgca acggtagatc ggcggtgacc atcccgagga aaggaggaga aaccgacatg
     2641 ctgagacgac ccgagaaagt cgagctggtc aacgagctga gcgagaagct gcgccaggcc
     2701 caatcgatca tcctgacgga ttttcagggg atgaacgtgg ccgaagtgag cgacctgcgt
     2761 tcgcaattgc ggtcgcaggc gatcgagatg cgcgtggtga agaatcggct gattcggcgc
     2821 gcgctatcgg acgcgggttg cgacagcctg gattcgctgc tggtgggcaa cacggcggtg
     2881 gtgttcggga tgagcgatcc cgtggcgccg gcgaagatcc tggtcaagta tgccaaggac
     2941 aatgagaagc tcaaggtcaa gggcgggctg ctcgagcgca aggcgcttga ttcggccggc
     3001 gtggacaacc tgtcgcggat gcccggacgc cacgagctgt tgacgatgat ggcgcgcgac
     3061 ctgaaggcgc ccgccacgaa gatggcgacg gtgttccagg ccgggctgct caaggtggcg
     3121 cacgcgatga acgccctggc gcgcaagaag gaagaagccg gcgaagcggc ggcgtgagcc
     3181 gcgcgcgacc gaaccgatag cccgatcccc gtttgaagcg agaaagatca aggagcaaga
     3241 gaacgatggc agagacgtta acgcccgatc aggtgctgga agcggtggaa cagtggtcgc
     3301 tgcttcaggt caacgaattc gtgaagaagt ttgaagagaa gttcgacgtg tcggcggcgg
     3361 cgccggtggc ggtggcggcc ggcccggtgg cgggcggcgg gggagaagcg gccccggcgg
     3421 ccgaggagaa gaccgaattc accgtcgtgc tgaaggcggt ccccgcggac aagaagatca
     3481 aggtgatcaa ggaagtgcgg gcgctgaagc cggacctggg cctgaaggag gccaaggacc
     3541 tggtggacgc ggcgccgaag gagttgctga ccgacgtgtc gaaggacgac gcggagaagg
     3601 cgaagaagca gcttgaggaa gccggagcgg aagtcgaatt gaaataaatt gcgactacgc
     3661 ggcggcaagc tggtaagatg gtgcaaggct cgggtctggc ccgccgggag gtgggatcag
     3721 acccggcccg gcatttcccc ggccgggggc cgaaggcgcc ccccaggtcc cgatctcatt
     3781 tgaaatcaaa tccaaccgct gacgccccca gcgtgcagac gccgggagct cggcccacgg
     3841 aggtccgatc agtctatggc ggccaaactg tcctccaacg gcaacgggcg cttctccttc
     3901 gcgcgcatcc ccgaaatcat ggagatgccg tatctgctgg agacgcagaa gctctcgtac
     3961 agtgaattcc tgcagatgaa tgttccgccc gacctgcggg cgaaccaggg gttgcaggag
     4021 gtgttcctgt cggtcttccc gatccaggcg ccgaacaatc cgtcgacgct ggaatttgtc
     4081 gagtatagtt tcggctcgcc gaagtacagc gtgcgggagt gcatcgagcg ggggatgacg
     4141 tatgccgtac cgctcaaggt caagctgcag ttgatcatcc gcgagacgga cgattcgacc
     4201 ggcgtgaccg aggtgaagga catcaaggag caggaaacgt acatgggcga gatcccgctc
     4261 atgacggagc aggggacgtt catcatcaac ggcgccgagc gcgtcatcgt gagccagttg
     4321 caccgatcgc cgggcgtttc gttctcgcag gggctgcact ccaacggcaa ggagattttt
     4381 tcggcgcgga tcattccgta tcgcggggcg tgggtcgagt tcgagatcga catcaacaac
     4441 ctgatgtggg tgatgatcga ccggaagcgg aagattccgg cgacgacatt tctgcgcgcg
     4501 ttcgggacgc acgccgacgc ggaattcgtt tccgagttct tcaagaccga gacggtcgcg
     4561 ctggacgact tctcgcgcgg cgggacgaag gttcaggacc tggatcagta cgtgggcgag
     4621 gaactgatcg aggaggtgat cgatcccaag agcggcaaga agctggccga gcgcggggcg
     4681 aaggtcacca agaagctggc cgagcaattg aagagcagcg gcgtgaagaa ggtgctgctg
     4741 acggtcgccg acgcgtatgc cgacctgctg gggcggatcc tggccgagga cgtcgttgac
     4801 acgcggacgg gcgagatcat cggcgagtac tgggagcggg tgtcgaccaa cttcctgcgt
     4861 catgcggccg agtgcaagat cagcaagatc aagctgctgg agcggatgca cgaagaaaac
     4921 gacacgatgc tgctgacgct ggagaaggac aaggtccgct cgtacgagga agcgctgatc
     4981 gagttcttca agaagatgcg tccgggcaac ccggtgtcgg tggcggcggg ccggcggctg
     5041 atggaagaca tgttcttctc ggagaagcgc tacgacctgg ggctggtggg ccgctacaag
     5101 atcaaccgga cgttcgggct gccggccgac gatcagagcc ggttgctgag cgcgcgcgac
     5161 gtggtggaag tgatgaagcg gctggtgtcg ctgcagcgcg atctggccga cgccgacgac
     5221 atcgatcacc tgggcaaccg gcgggtgcgg tcggtgggcg agctgctgca gaaccagatc
     5281 cgcgtcgggc tggccgagct ggagaagacg gcgcgggagc ggatggcgat catcgagctg
     5341 gacaacctgc tgccgcacaa cctgatcaac gccaaaccgg tggtgtcggc gatcaaggac
     5401 ttcttcgggc gcagccagct cagccagttc atggaccaga ccaacccgct gagcgagctg
     5461 acgcacaagc ggcggctgtc ggcgctgggt ccggggggtc tgtcgcgcga ccgggcgggg
     5521 ttcgaggtgc gcgacgtgca ctattcgcat tacgggcgga tctgcccgat cgagacgccc
     5581 gaaggtccga acatcgggct gatctcgtct ttgtcgacgt atgcgcggat caatcagttc
     5641 gggtttatcg aaacgccgta catcaaggtg aaggacagca aggtcacgag cgagatcgag
     5701 tacatgacgg cggacgtgga ggacgaattc cacatcgcgc aggcgaacgc ggaggtcgat
     5761 ccgaagacgc gcaaattcgt caacgacctg gtgctgtgcc gctatcgcga cgattacccg
     5821 cgcgtgccga acagccaggt cgactacatg gacgtctcgc cgaagcaact cgtgtcggtg
     5881 tcggcctcgc tgatcccgtt cctggagcac gacgacgcca accgggcgct gatggggtcg
     5941 aacatgcagc gccaggcggt gccgctgctc tacacgagca gcccggtggt gggcacgggc
     6001 gtggaacgca aggcggcgat ggactcgggc gtgtgcatca tcgcgcggac aagcggcgag
     6061 gtgatcagcg tggtggccga cgagatcgtc gtgcgcaacg aactgggcgg gacggatcac
     6121 tacaagctgc gcaagttcca gcggtccaac cagaatacgt gcatcaacca gaagccgatc
     6181 gtctaccgcg ggcagaaagt gcaggcgggc gacgtgctgg ccgacgggcc agcgacggaa
     6241 gcgggcgagc tggcgctggg caagaacctg atggtggcgt tcatgccgtg gggcggctac
     6301 aacttcgaag acgcgattct gttgagcgag cggctggtca aggacgacgt cttcacttcg
     6361 atacacatcg aggaattcga gatcgacgcc cgcgacacca agctgggcaa ggaagagatc
     6421 acgcgcgaca tcccgaacgt gagcgaggac gcgctgtcga agctggatga agagggtctg
     6481 gtgttcatcg gcgcggaggt cgtgccgggc gacatcctgg tgggcaaggt gacgcccaag
     6541 ggcgagaccg agctgacgag cgaggagaag ctgctgcggg cgatcttcgg ggagaaggcg
     6601 ggcgacgtgc gcgacgccag cctgaaggtt ccgccgggga cgcaggggac ggtgatcgac
     6661 atcaaggtct tcagccggaa ggaacggacg gggcggaccg atcgccagga caagcagaag
     6721 atcgcggacc tgaatcgcga acgcgacgag gggatcgcgc agctgtggtc ggaattcgat
     6781 ctggagctgc aggagctgct caaggacatc gatcgcccga tcgtgaattt tgaaacgggc
     6841 aagaccgagt tgaagccggg gggcaaggtg acggcggcgg tgatcgacta cgtgcgccat
     6901 tgcctgcgga ccggcatgct gccgatcgac gggccgaagg ggatggaggt caagcggctg
     6961 taccagcgga cgatcgcgcg cgagacggag atcaacgacc agacgaacaa caagatcgac
     7021 cggatcaaga cgggcgacga gctgccgccg ggggtcaaca agctggtcaa ggtgtacctg
     7081 gccacgaagc gcaaggtgtc ggtgggggac aagatggcgg gacgccacgg caacaagggt
     7141 gtggtggcgc ggatcctgcc ggaggaggac atgccgttcc tggccgatgg gcggccggtg
     7201 gacatcgtgt tgaacccgct gggcgtgccg agccgaatga acgtcggtca gatcctggag
     7261 acgcacctgg gctgggcggc ggcggcgctg gggatgaaga tcgcgacacc ggtgttcgac
     7321 ggcgcgcggg aggagcagat caaggaatgg ctgcgccggg cgcagttgcc cgagagcggc
     7381 cagacgacgc tttacgacgg gttgaccggg gaggcgttcg accgccaggt gaccgtcggc
     7441 atcatctaca tgatgaagct ggcgcacctg gtcgacgaca agatgcacgc gcggtcgatc
     7501 ggaccctatt cgctcgtgac gcagcagccg ctgggaggca aagcgcagtt cggcggccag
     7561 cgcttcggcg agatggaggt ctgggcgctg gaagcctacg gggcgtcgca cacgctgcag
     7621 gagctgctga cggtcaagag cgacgacgtc cagggccgca cgcgcgtgta tgaagcgatc
     7681 gtgaagggga tgaatgcccc aacgccgggg gtgcccgaat cgttcaacgt gctggtgaag
     7741 gagctgcagg ggctatgtct gaacctggag ctgctcagca cgaaggacct ggaggaagag
     7801 acggcggcgg cggaacaggg cctggaggcg atggccgagg ggctgctgcc ggcggggctg
     7861 gtggacgagg acgaggacga agaagaggac gtctcggcgg aggtcagtgc aggggacgag
     7921 gacgaagagc tgtgagggat ggcgggggcg gggcgcggcg ctcctccccg ccgactcgat
     7981 cgcggcggcg caagggaacg tttttcgaat caatcaccag atccgcctcg ggcgtcgctc
     8041 gcgtccggcg tgaggcgaat caaaggggaa acgaagcatg gcaacagccg gtatcacggg
     8101 ttcgcagcgc acgatgtttc agccgggggc cccgacgggg cggtccgaac gcattgatga
     8161 gaccaatgcg atctgccgga tttcgctggc ctcgccccag cagatcctga agtggtcgag
     8221 ccgccagcgg cgccaggcgg agcatctgag caacctgcag cttggccgcc tgggcgacga
     8281 gatcgatccg accagcttcg gggaagtgaa gaagcccgag acgatcaatt accggacgtt
     8341 caaaccggag aaggacgggc tgttctgcga gcggattttc ggtccggtga aggactggga
     8401 gtgcgcctgc ggcaagtaca agcggatcaa gtacaagggg atcgtctgcg atcgctgcgg
     8461 cgtggaggtg accgagagca aggtccggcg ccaccggatg ggccacatca agctggcggc
     8521 gccggtctgc cacatctggt tctacaaggg gacgagcagc cggatcgcca acctgctgga
     8581 catatcggcg cgcgacctgg gcaaggtgct ctatttccag gactacatcg tcgtggagcc
     8641 gggcgacacg ccgctgaagg agaagcagat cctgtcggag gaagaggcgc gcaagtacag
     8701 cgaggtctac ggcgacaagg tcaagatcat gatcggcgcc gaggcggtgc gcgagctgct
     8761 gaagaagatc aacatcgagg aagaagccaa ccggctgatg ctggagatgc gccagagcga
     8821 ttcgctgcag aagcggacca agatcatcaa gcggctgaag gtcgtggagg cgttccgcaa
     8881 gtcggacaac cggccggagt ggatgattct ggaagtgctg ccggtgattc cgccggacct
     8941 gcgcccgctg gtccacctgg acggcggccg gttcgccacg tcggacttga acgacctgta
     9001 ccggcgcgtg atcaaccgga acaaccggct gaagcggctg atcgagctgc acgcgcccga
     9061 cgtgatcctg cgcaatgaga agcggatgct gcaggaagcg gtcgacgcgc tgttcgacaa
     9121 cagccgccgg tcgcggccga cgaaagggca caacaaccgg ccgctgaagt cgctgtcgga
     9181 catgctcaag gggaagcagg ggcggttccg gcagaacctg ctgggcaagc gggtcgatta
     9241 ctcgggccgg tcggtgatcg tggtgggtcc ggatctgaaa ttcaatcagt gcggcctgcc
     9301 gaagaagatg gcgctcgagt tgttcgatcc gttcatcatc cacgagctgc agaagcgggg
     9361 ccactgcaac acgatcaagt cggccaagcg gatgatcgag aaggaagagc cggtcgttta
     9421 cgatattctg gacgacatca ttcaggatca cccggtgttg ctgaaccgtg cgccgacgct
     9481 gcaccggctg ggggttcagg cgttcatgcc gaagctggtc gagggcaagg cgatccagct
     9541 ccatccgctg gcctgcgcgg cgttcaacgc ggacttcgac ggcgaccaga tggcggtgca
     9601 cgtaccactg tcgaccgaag cgaagctgga agcgcggctg ctgatgctgg cggagaacaa
     9661 cattctctcg ccggccaacg ggttcccgat cgccagcccg agccaggaca tggtgctggg
     9721 ctgcttctac gtgacgaaga tggtgcccgg cgccaaaggc gaagggatgc ggttcgccga
     9781 tccggacgag gcgttgatgg cgtaccagta tgaacgggtg gcgctgcagg ccgaggtcga
     9841 ggtgcggatc gacggcannn nnnnnnnaga cgacgccggg gcggatcata ttcagccgcg
     9901 ccctgccgaa cgagatccgg tacttcgacg aggattcgcc gtatttcaac aaggtgatga
     9961 tcaagaagac gctggtgcag ctcgttgacg acgcgcaccg gcggtgcggc gcgcggcgga
    10021 cggctcagat gctcgacgcg atcatgcagc tgggcttcaa cttcgccaag cgcggcggca
    10081 tgtcgatctg cgtcgacgac atgctgattc cggagtccaa gccgaagatc atccgcgagg
    10141 cggtcaagaa cgtggcccgc gtgcagaagc gttacgagca gggttcgatc acgaacgagg
    10201 agcgctactt cgacgtgatc aacatctggt cgcacgcctc ggaagcggtg gcgaaggaca
    10261 tgacgctggg gctggagaag gaccagtcgg gactgaaccc gatctacatc atggctcact
    10321 cgggcgcgcg tggcaacatg gcgcagatcc gccagcttgc cggcatgcgc ggactgatgc
    10381 agaagccgac gaagaagctg acgggcggcg tgggcgagat catcgagtcg ccgatcatgt
    10441 cgaacttccg cgaggggctg accgtgctgg agtacttcat ttccacgcac ggggcgcgca
    10501 agggcctggc cgacacggcg ctgaagacct ccgacgcggg ttatctgacg cgccggctgg
    10561 tcgatatcgc gcaggacctg atcgtgacgg aggaggactg cgggacggac gacgggatcg
    10621 aggtcgagcc gatcaaggag atcacggccg agggcgagcg aatcctggag ccgctgcgcg
    10681 accgcatcgt cggccgcgtc gccgtccgga cgatgaagaa ccccgtgacg aacaaggtgg
    10741 tggtgcatcg caaccaactg atcgacgagc agacggcgcg cgacatcgag aacgccggtt
    10801 tcgagcggat cctgatccgt tcggtgctga cgtgcgaaag ccgccggggc gtctgcgcga
    10861 agtgttacgg acggaacctg gccaccggcc aggacgtgga gatcggcgag gcagtgggcg
    10921 tgatcgcggc gcagtcgatc ggcgagccgg gcacgcagtt gacgctgcgc acgttccaca
    10981 tcggcgggac cgccagcctg cagcttgagg gttggtacga ggcggtcgag gacggcatcg
    11041 tgcactatcg cgaggggctg cgcaccgtgc cgcgcgagga ggagacgggc gagatcgtca
    11101 acgtcgtgat cgtgcggacc ggcaccgtga cggtcgagga caagaacggc aacgagatcc
    11161 aggtgctgcc gaacatcccg tacggcgcga cgatcacgaa ggccgagggt caggaagtca
    11221 agaagggcga gaaattcgtc cagtgggata cgaaccagac gccgattatc gccgagagcg
    11281 acggcatcgt gaagttctcg gacattatcc ggggggtcac ggtggccgag gatatcgact
    11341 cgcggacggg caacgtgcag ctcgtg
//
LOCUS       DAOPCO010000118        11243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5679_length_11243_cov_5.851448, whole genome shotgun sequence.
ACCESSION   DAOPCO010000118 DAOPCO010000000
VERSION     DAOPCO010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5679_length_11243_cov_5.851448"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLS90_09505"
     CDS             <1..291
                     /locus_tag="PLS90_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02680.1"
                     /translation="IPATWIDSGMTAPVYPTQGVPGCSACFAVGQLDADPQEEIVYTN
                     DDGFLVIVDLVDQTETELSVGPGDGSEYVGLSIGNFMPDYLVPAELSSFAVE"
     gene            421..1593
                     /locus_tag="PLS90_09510"
     CDS             421..1593
                     /locus_tag="PLS90_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02681.1"
                     /translation="MFGLKTHSDRAIRLAAFAIVALAAAPSQALDEIAWLDLEGGLHL
                     LTPDGNTAGTFADTQTVPGSSESNIFLVAYDRDKDGLDELIIGTTDESRPSDIGLLVW
                     DPKLNSGAGGVVVPSSNLNVISMPGIMEHLGAIATINPEGTPREVVVQGAINYHSSFT
                     FLDGSARDANNNIVADSTYGGWQVYDLDGNGADEIFYFRGTGLYYREYDGQANVDEVF
                     YANIPIPGGPFQRWNFLLLIGDVDGDSEPDILFGPPDPNDDGHMLVYYHPLLVPEGDL
                     PLEVEDLIDSGMEISRHAAPGGGRGGGGCLYTLGQMDADPQLELLHINDDGWIAIIDL
                     VDKTETVLNGVGPGVSNRENGEYSLTTAGYSALTTGNFSSTGGGEIPNRARHWSLY"
     gene            1709..1770
                     /locus_tag="PLS90_09515"
                     /pseudo
     CDS             1709..1770
                     /locus_tag="PLS90_09515"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            2088..2303
                     /locus_tag="PLS90_09520"
     CDS             2088..2303
                     /locus_tag="PLS90_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02682.1"
                     /translation="MEGYEYADPYYVAAMRLFPDWANGTYGYKITENVQWRLTSPGPT
                     QMYWSLGLAYDPSNGTVSPGVIIRMGP"
     gene            complement(2327..3259)
                     /locus_tag="PLS90_09525"
     CDS             complement(2327..3259)
                     /locus_tag="PLS90_09525"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK02683.1"
                     /translation="MASSFCFHDLAKPRLLHDFKNTIFTLFHLPLNFRNPVRPEETLF
                     EREPDRWSPVYRFLWDTRQGRRDSETLATKLVGEALRCGSPAKLKCFAGLTHISIPIV
                     IDGKVEMVLLTGDFLTGPPDSQYIDSLVTLARRYGGNPDPILQGIQTVPILPVETVDS
                     LINLLVIFCDYIALLGEDLLLKRRIGESDPVGQIREFIQNHYREKLTLNRIASEVHLS
                     STYVSRLFRRQSAQTLTAYQNEVRIAEARRLLATTRMRIGEICYEVGYQNLSHFTRNF
                     KNLVGVSPSVYRNEHLNSQRRMAHLDDELTRNRC"
     gene            3540..4736
                     /locus_tag="PLS90_09530"
     CDS             3540..4736
                     /locus_tag="PLS90_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02684.1"
                     /translation="MTAHALPLAQPPFRGAQLPAARTVFGRASYILSSQRVQAALTKT
                     GGHLGPVSFDLGDRAIQPYAIAPWWNERQPPERPVMQRVLRGDFFCLPFGGCHGGDNH
                     MPGVPTHGDTAARRWRFRDFDQSEERTWIELEMRVRYPRAHVLKRIELRVDHTAIYQS
                     HVIRGVSGPICFGHHGMLQFPDGCEAAYLSSSPFPRGQVLPDQGDDTASRERNFLRPG
                     SCFTRLEAVEDIHGGLTDISRFPARRGYDDLVMLAADPSLPFAWDAVSNPTLGYVWFS
                     LRDPAVLASTILWLSNGGIDYPPWNGRHMNVLGLENVTSYFDYGLHASSGPNPFSDLG
                     FKTSVTLSPDQSLAVRAIFGVAAVPRTFSKVDSIVPVAGGIELVDSLGARVRTPLDLE
                     FIAGEG"
     gene            5067..5894
                     /locus_tag="PLS90_09535"
     CDS             5067..5894
                     /locus_tag="PLS90_09535"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPK02685.1"
                     /translation="MSAATPTNGSDGSARRARFQGKVVLVTGSSRGIGREIAAAFAAE
                     GAHVVITSCDAGEVHAVHRQFEEHGWSAMPCVADVRDAEAIARMVEAILARWSAIDIL
                     VNNAAIFPKRIPFLEIPFENWREVIDVNLFGVVRVTQAVGRIMARQPEGGRIVNISSL
                     NASRYRVTTIGQTQYNVAKAALDNLTMGLAMELAPHGIIVNAIAPGFVDSTTLGKEDG
                     QDDDPLRQDYLEGGRIPLRRYGQPRDCANLVLFLASPECTYMAGETVRIDGGLSFIF"
     gene            complement(5924..6940)
                     /locus_tag="PLS90_09540"
     CDS             complement(5924..6940)
                     /locus_tag="PLS90_09540"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPK02686.1"
                     /translation="MASRHRPPAEPSAPAARGAAPDAGTRRRRLVIYGNPQVPGVGAV
                     LAELWAWAAARGMALSIADDIVALAAAPPGAAAHESYPAEDGSRIMARPDEDPLLVCL
                     GGDGTILHAVRHFWPFRGPVLGVNLGSLSFNAAIDPERVTQVVENWMAGRAAVSRRIL
                     LQARLTRGGQVTEQGIAVNDLVVSKHADARVIHLTMRQGEELVSAFIGDGLIVSTPTG
                     STAYNLSAGGPIVYPTLEAIVATAICPHTLASRPVVLPPAPPLTLQFVPHHGRDQATI
                     WLDGQVAWEFRQGDEVSIGPADRPIDLLVGTDMSYFERLRSKLAWSGEMRPAGLVQEG
                     SGAG"
     gene            complement(6949..7779)
                     /locus_tag="PLS90_09545"
     CDS             complement(6949..7779)
                     /locus_tag="PLS90_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPK02687.1"
                     /translation="MDQLLVDRALAESRERAQRLIRAGLVIVGEQRVDKPGQRVDADA
                     PVRLKGEPCPYVSRGGLKLAGALRRFGLTDLRGRIAVDVGASTGGFTDCLLQHGAATV
                     WAIDTGRGQLHARLREDPRVRLLERCNARYLAPDWLDEQRVDLIAIDVSFISLRLILP
                     PLPPLLAPAGSVVALIKPQFEAGPADVGRGGVVRERRVHRRVLADVLEFAAQTGWSAV
                     GLCPSPLRGPAGNLEFFAHLRRRAGGDPSATLAENAIERVLDEAYTQEATPRAAEPGA
                     "
     gene            complement(7882..8313)
                     /locus_tag="PLS90_09550"
     CDS             complement(7882..8313)
                     /locus_tag="PLS90_09550"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK02688.1"
                     /translation="MTEEHAAVAPGPGLLSRLGELGRSDEEANRGLPDLEHDWEFALF
                     GIRYEVIDQVQRALEAEGLSQAELARRMGVSRAYVSKILNEQANFQMETIAKLAVALN
                     REIHIRFHEKPGEPVARRKAARGRKASSTATRVRKSKANPG"
     gene            complement(8329..8748)
                     /locus_tag="PLS90_09555"
     CDS             complement(8329..8748)
                     /locus_tag="PLS90_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02689.1"
                     /translation="MGQEGGRRKAIRLLQIGPRCDVLIELAAWFKSSPREIKRVLKKL
                     QMIAEYDDLPRLSTIKRVGGTGRIIQISGGNGRLYCFLDRNGMTTVICVNTFWIGGGD
                     KLKRQSAEIEEAERRMLLWQSARIVSGIVGARIARAG"
     gene            complement(8874..9509)
                     /locus_tag="PLS90_09560"
     CDS             complement(8874..9509)
                     /locus_tag="PLS90_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02690.1"
                     /translation="MTLACLLPLSALGGCQARFPAERIPKMKWLILPFEQPPSMSTTP
                     RAIRGWWFGARTVRQNPRAGQMIADSLNRAMAEYDFINLYSAIDLRYYFADKRQILED
                     TYPHLSEGEIQDLLGQVDPLDYGKELGADKILTGRIIRQYLAENRTIHWWWSVAEIEA
                     QVIDVQTGRVEWSERYDVRTQLASQSAVIDDVVARMLEDLKEDYFLPLSRR"
     gene            9798..10907
                     /locus_tag="PLS90_09565"
     CDS             9798..10907
                     /locus_tag="PLS90_09565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02691.1"
                     /translation="MHAPLTPVPLRPAERGVLAVVLAGALLLRALDALLWHPFTPDEE
                     FTLALGRMHWGEMLRATAADTHPPLYYALVKLVFLLTPDEYAPARLLSVALAGATLWL
                     IFRFARDGFSPAAGWVALLCASFAPYMIYWQHAARNHQLLPPAVLLIIGLSYRYASGG
                     GRARWWALAAAWLVAIQTNYMALVFGLVWGAAFILAEPMAPRRRARLAATPLPGLLSF
                     VPWLGILGRQVQAGPMNKGFFQETVSPIYLYFHALFGRMEPYQPNQTGPLFLLALLAF
                     ALVAVLGARAVGHRWSFWVLLLGLPTMPIVAAKAAGWTLAERHLAFALPVFFVYWGAA
                     CVATVRQLHARCRAPGVQQPTGEETAPCSADSKPK"
     gene            10880..>11243
                     /locus_tag="PLS90_09570"
     CDS             10880..>11243
                     /locus_tag="PLS90_09570"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK02692.1"
                     /translation="MQRRLEAEVMDTEAEAVDYDRMDHSEPNEAFVARLIELGACGRM
                     LDIGTGPGHIPLLVCERIRDAEVLGVDLADAMLAIAERRRRASAHRERLRYERADACA
                     LPYADGAFDAVFSNTILHH"
BASE COUNT         1831 a         3991 c         3460 g         1961 t
ORIGIN      
        1 atccccgcca cctggatcga cagcggcatg accgccccgg tatatccgac ccagggcgtc
       61 ccagggtgct cggcgtgctt cgcggtcggt cagctcgacg ccgatccgca ggaggaaatc
      121 gtttacacca acgacgacgg cttcctggtc atcgtcgacc tggttgatca gaccgagacc
      181 gaattgagcg tcggccccgg cgacggcagc gaatatgtcg gtctgtccat cggcaatttc
      241 atgcccgact acctggtccc ggccgagctg tccagcttcg ccgtggagta agcgtcacgc
      301 aaggctggcg tgcagcctga gttaaaagcg gggtgatcgg tcggccggcc gatcaccccg
      361 gcacgccaga tccccggccg cgccggcacc cgttggaacc gaatcgcaaa ggagacgcat
      421 atgtttggcc tgaagaccca ttctgatcgg gcgatccgac tggccgcatt cgccatcgtc
      481 gctctggccg ccgcgcccag ccaggccctc gacgagatcg cctggctcga cctcgagggg
      541 ggactgcacc tgctcacacc cgatgggaat accgccggca cctttgccga cacccagacg
      601 gttcccggca gctcggagtc caacatcttc ctggttgcct atgaccgcga caaggacggc
      661 ctcgacgagc tgatcatcgg caccaccgat gaaagccggc ccagtgacat cgggctgctg
      721 gtctgggacc cgaagctcaa cagcggggcc ggcggcgtcg tcgtgccgtc gtcgaatctc
      781 aatgtcattt cgatgcccgg catcatggag cacctggggg cgatcgcgac gatcaacccc
      841 gaggggacgc cgcgcgaggt cgtcgttcag ggcgcgatca attatcactc ctcgttcacc
      901 ttcctggatg ggtcggcgcg cgatgcgaat aacaacatcg tggccgattc gacctacggc
      961 gggtggcagg tctacgacct cgacggcaac ggcgccgacg agatcttcta tttccgcggc
     1021 acgggcctgt actaccgcga gtatgacggc caggccaacg tcgacgaagt tttctatgcc
     1081 aacatcccca ttcccggcgg gcccttccaa cgctggaatt tcctcctgct gatcggcgac
     1141 gtcgacggcg acagcgagcc cgatatcctc ttcggcccgc ccgacccgaa cgacgacggc
     1201 cacatgctgg tctactacca cccgctcctt gtccccgagg gggacctgcc cctggaagtg
     1261 gaagacctga tcgacagcgg catggagatt tcacgtcacg ccgcgccggg cggcggtcgc
     1321 ggcggcggcg gctgcctcta caccctcggt cagatggacg ccgacccgca gttggaactg
     1381 ctgcatatca acgatgacgg ctggattgcc atcatcgacc tggtcgacaa gaccgaaacc
     1441 gtcctgaacg gcgtggggcc gggcgtcagc aaccgtgaga acggcgaata ctcactgacg
     1501 accgccggct actccgccct caccaccgga aacttcagca gcacgggcgg gggcgaaatc
     1561 cccaatcgcg cacggcactg gagcctctat tgatccgccg ctttgggcgc cggggccggc
     1621 ggacgccgcc ccggcgctcc gatccctcga ttctcttgca agaaaggtcc aatcaacgat
     1681 gagcccgcga tctctcccaa cccgggtgat gtccacgccg agggtcccgg cgcgcgcctt
     1741 caccctcatt gaattgctga ttgtggtcgc gatcattgcc atcctggccg cgatcgccgt
     1801 ccccaatttc ctcgaggccc aggtccgcgc caaggtcagc cgcggccatt ccgacatgcg
     1861 cagcatggcc atcgccctgg aaagctatcg caccgaccac aatcagtatc cgctgacttc
     1921 gtggtactcc tggaacgccg gcaagttgcc caactcctac tccaacaaca cgaccaccca
     1981 gcacgactcc tactactgtc tgacgatgct gaccacgccg gtggcttata ccaccagcct
     2041 cgggcgtcat ccgttctggc cgtcgccccc ccgggtcgag ctggccgatg gaaggctacg
     2101 aatacgccga cccgtattat gtcgccgcga tgcgtctgtt ccccgattgg gccaacggca
     2161 cctacggcta caagattacc gaaaacgtcc agtggcgcct cacgtctccc ggcccgaccc
     2221 agatgtactg gtcgctcggc ctggcctacg atccgtccaa cgggaccgtc agcccgggcg
     2281 tcatcatccg gatggggccg tgacgtcctg ccgcggctcc cgctcgtcag cagcggtttc
     2341 tggttaactc gtcgtccagg tgggccatcc ggcgctggct gttgagatgt tcgttgcggt
     2401 agacgctggg cgaaaccccg accaggttct tgaagttgcg cgtgaaatgg gaaaggttct
     2461 ggtagccgac ttcgtaacag atctcgccga tccgcatccg cgtcgtcgcc agcagccgtc
     2521 gcgcctcggc aatccgcact tcattctgat acgccgtcag cgtctgcgcc gactggcggc
     2581 ggaacagccg gctgacatac gtcgaactga ggtgaacttc cgacgcgatc cggttcaggg
     2641 tcagtttctc ccggtaatgg ttctgaatga actcccggat ctgcccgacc ggatcgctct
     2701 cgccgatgcg ccgtttcagc agcaggtctt cgcccagcag cgcgatgtag tcacagaaga
     2761 tgacgagcag attgatcagg ctgtcgacgg tttcgaccgg gaggatcggg acggtttgaa
     2821 tcccctgcag gatcgggtcc gggttgcccc cgtagcgacg cgccagcgtc accagcgagt
     2881 cgatgtattg cgaatcgggg gggccggtca gaaagtcgcc ggtcagcaga accatctcca
     2941 ccttgccgtc gatcacgatc gggatgctga tgtgcgtcaa ccccgcgaaa cacttcagct
     3001 tggccggcga cccgcagcgc agcgcctcgc cgaccagctt cgtggcgagc gtttcgctgt
     3061 cgcggcgccc ctggcgcgta tcccacagga accggtaaac cggcgaccag cggtccggtt
     3121 cgcgctcaaa cagcgtctct tccggccgca ccggattgcg gaaattgagc ggcagatgga
     3181 acagcgtgaa gatcgtgttt ttgaagtcgt gcagcagtcg aggctttgcc aggtcatgga
     3241 aacaaaagct cgatgccatt ccagcaacct cccccgcctc agggatcgat tcatactgat
     3301 ggataccctt aagaataaca ggaagcccgg gagacgcgcc atcagctttt ttcccggcgg
     3361 cccgcgcggg cgtttccggg gtgtttgccg gttttgcgtg ccaacggcaa gaaagggcaa
     3421 tgttttcacg ccagactggt tgtccgccca gcccccgtgc gggcaccatg atccgcgaat
     3481 tgccgccgat cgggctctcg gattcctggg gcagttcatc catccgtgaa aggcgtccga
     3541 tgacggcaca tgctctccca ttagcccagc ctccgttccg cggcgcgcaa ctccccgccg
     3601 cccggacggt cttcgggcgc gcctcctaca tcctctccag tcagcgcgtc caggccgccc
     3661 tcaccaagac cggcggccat ctggggcccg tcagtttcga cctcggcgac cgcgccatcc
     3721 agccctacgc catcgccccc tggtggaacg agcgtcagcc tcccgagcgc cccgtgatgc
     3781 agcgcgtcct gcgcggcgat ttcttctgtc tccccttcgg cggctgccac ggcggcgaca
     3841 accacatgcc cggcgtgccg acccatggcg acacggccgc ccgccgctgg cgcttccgcg
     3901 acttcgacca gagcgaggag cggacctgga tcgaactgga gatgcgggtg cgctatcccc
     3961 gcgcccatgt cctcaagcgg atcgagctgc gcgtcgatca taccgccatc tatcagtcgc
     4021 acgtcatccg cggcgtcagc ggcccgatct gcttcggcca tcacggcatg ctgcaattcc
     4081 ccgacggctg cgaggcggcc tatctttcct ccagcccctt cccgcgcggc caggtgctgc
     4141 cagatcaggg cgacgacacc gcgtcgcgcg agcgcaactt cctgcgcccg ggaagctgct
     4201 tcacgcggct cgaggcggtc gaggatattc acggcggcct gaccgacatc agccgctttc
     4261 ccgcgcgtcg cggctacgac gacctcgtca tgctggccgc cgatccctcg ctcccgtttg
     4321 cctgggacgc ggtttccaac cccaccctgg gctatgtctg gttctcgctg cgcgatccgg
     4381 ccgtgctcgc ctccaccatc ctgtggctgt ccaacggcgg catcgactat ccgccgtgga
     4441 acgggcggca catgaatgtg ctcggtctgg aaaacgtgac gagttatttc gattacggtc
     4501 tccatgcctc cagcggcccc aatccgtttt cggacctcgg tttcaagacc agtgtcacgc
     4561 tcagccccga ccagtcgctc gcggtccgag cgatcttcgg cgtggcggcg gtcccgcgca
     4621 cgttcagtaa ggtggattcg atcgtgccgg tcgccggcgg catcgagctg gttgactccc
     4681 tcggcgcccg agtccggaca cccctggatc tcgagttcat cgccggggag ggttgaggcc
     4741 gctccctcaa cccgcggggt tttcgagagc cgaagacgaa ggacgtgagc cgcgcgccgg
     4801 attttggaga aggcctttgg gggcgattac gaggacgagg gacggaggga tcaccgcgcg
     4861 aaatgggtcg acgaagcttt ccgcgatcta tctgctggca atccaagctg gattcgggcc
     4921 atcatgctca cggtttccaa ccggatgatt ctcactttgg cacaagatcc ggcgaccgga
     4981 tgccaccccc cgctcgccgc aacgggccgt cgcccgctcg gcctcacggg ccgattgtgc
     5041 cctgaagttc acctggggag gattgcatga gcgccgccac gcccaccaac ggctccgacg
     5101 ggtccgcccg gcgcgcgcgc ttccagggca aagtcgtcct ggtgaccggt tcgagccgcg
     5161 gcatcggccg tgaaatcgcc gccgccttcg cggccgaagg cgcgcatgtc gtcatcacct
     5221 cgtgcgacgc cggcgaggtc cacgcggtcc atcgccagtt cgaagagcac ggctggtccg
     5281 ccatgccctg cgtcgccgac gtgcgcgacg ccgaggccat cgcccgcatg gtcgaggcga
     5341 tcctcgcccg atggtccgcg atcgacattc tggtcaataa cgccgcgatt ttccccaagc
     5401 gcatcccgtt cctggagatc ccgttcgaga actggcgcga agtgatcgac gtcaatctgt
     5461 tcggcgttgt gcgcgtcacg caggccgtcg gccgcatcat ggcccgccag cccgagggcg
     5521 gccggatcgt caacatctcc tcgctcaacg ccagccgcta tcgcgtcacc acgatcgggc
     5581 agacccaata caacgtggcc aaggccgccc tcgacaacct gacgatgggg ttggcgatgg
     5641 aattggcgcc gcacggcatt atcgtcaacg ccatcgcccc cggcttcgtc gacagcacga
     5701 ccctgggcaa ggaggacgga caggacgacg accccctccg ccaggattat ctcgaaggcg
     5761 gacgcatccc gctgcgccgc tacgggcagc cgcgcgactg cgccaacctg gtgctcttcc
     5821 tggcgtcgcc cgaatgcacc tacatggccg gcgaaaccgt ccgtatcgat ggcggcctga
     5881 gtttcatttt ctgaatttct gatccgaacc cgccgccccc gcctcagcct gcaccgcttc
     5941 cctcctgcac gagccccgcc ggccgcatct cgccgctcca ggccagcttg ctccgcaggc
     6001 gttcgaaata actcatgtcg gtcccgacca gcaggtcgat cggccgatcc gccggcccga
     6061 tggaaacctc gtcgccctgg cggaattccc acgccacctg gccgtcgagc cagatcgtcg
     6121 cctgatcgcg tccgtggtgc ggcacgaact gcagcgtcag cggcggcgcg ggggggagca
     6181 cgaccggccg cgacgccagc gtgtgcgggc agatcgccgt ggcgacgatc gcctccagcg
     6241 tcgggtagac gatcggcccg cccgccgaca agttgtaggc cgtcgagccg gtcggcgtgg
     6301 agacgatcag cccatcgccg atgaacgccg aaacgagctc ttcgccctgg cgcatcgtca
     6361 ggtggatcac gcgcgcgtcg gcgtgcttgc tcacgaccag gtcgttgacg gcgatcccct
     6421 gttcggtcac ctgcccgccg cgcgtcagcc gcgcctgcag cagaatccgg cggctgacgg
     6481 cggcccgtcc ggccatccag ttctccacca cctgtgtcac gcgctcggga tcgatcgccg
     6541 cgttgaagct cagcgatccc aggtttaccc ccagcaccgg gccgcgaaac ggccagaaat
     6601 gccggaccgc gtgcaggatc gtcccgtcgc cccccaggca gaccagcagc ggatcctcat
     6661 ccggccgggc catgatccgc gagccatcct cggccgggta ggattcgtgc gccgcggcgc
     6721 cggggggcgc ggcggccaac gcgacgatgt catcggcgat gctcagcgcc atgccccgcg
     6781 ccgccgccca ggcccacagc tcggccagga cggcgccgac cccgggcacc tgcgggttgc
     6841 cataaatgac aagccggcgc cggcgcgtac cggcgtccgg ggcggcgccg cgcgccgcgg
     6901 gggcggaggg ttcggccggc gggcggtgtc gggatgccat ggggcgggtc acgctccggg
     6961 ttcggctgcg cgcggggttg cttcctgagt ataggcttcg tccagaaccc gctcaatcgc
     7021 gttctccgcg agcgtggcgg acgggtcgcc gccggcacgc cggcgcagat gggcaaagaa
     7081 ctccagatta ccagcgggtc cccgcagcgg cgacggacac aacccgacgg cgctccatcc
     7141 ggtctgtgcc gcgaattcga gcacatcggc caggacgcgc cggtgaaccc ggcgctcgcg
     7201 cacgacgccc ccccggccga catcggccgg ccccgcctca aactgcggtt tgatcagcgc
     7261 cacgacgctc cccgccggcg ccagcagcgg ggggagggga gggaggatca acctcagcga
     7321 gatgaaggaa acgtcgatcg cgatcagatc gacccgttgc tcgtcgagcc agtccggcgc
     7381 cagatagcgg gcattacacc gctccagcag ccggacacgc ggatcctcgc gcagccgggc
     7441 atgcagttgc ccgcggccgg tatcgatcgc ccagaccgtc gccgcgccgt gttgcagcag
     7501 gcaatcggtg aaaccgccgg tcgaggcgcc gacatcgacg gcgatgcggc cgcgcaagtc
     7561 ggtcagcccg aaccgccgga gcgcgcccgc cagcttgagg cccccccggc tgacatacgg
     7621 gcacggctcg cccttcagcc ggaccggcgc gtcggcgtcg acccgctggc cgggcttgtc
     7681 gacgcgctgt tcgccgacga tcaccagccc ggcgcggatg agccgttgcg cccgctcgcg
     7741 actctccgcc agcgcgcgat cgaccagaag ctgatccacg cgaatcttca tgcaccgccg
     7801 gccaaagccc cttcccgccg ggagcgtcga gccagtttca tcgtttcacc aagccgccga
     7861 cccgaggcgt caatggctcg ctcatcccgg attcgccttg ctcttgcgca cgcgagtggc
     7921 ggtcgagctg gcttttcgcc cgcgcgccgc tttccttctg gcgacgggct cgcccggttt
     7981 ttcatgaaac cgtatatgga tttcccggtt cagcgccacc gcgagtttcg cgatggtttc
     8041 catctggaag ttggcctgtt cattgagaat cttgctgaca taggcccgcg agactcccat
     8101 ccgccgcgcc aattctgctt gcgagagccc ttcagcttcc agcgcgcgct gaacctggtc
     8161 gatcacctca tagcggatcc cgaacagcgc aaattcccaa tcatgctcca gatcgggcag
     8221 accccgattt gcctcttcgt ccgagcgccc cagctcgccg agccgactca acaggccggg
     8281 tcccggggca acggctgcgt gctcttccgt catgagcttc gcttctcctt atccggcgcg
     8341 cgcgatacgc gcccccacaa tccctgagac gatccgggca ctttgccaaa gcaacatacg
     8401 gcgttcggct tcctcaatct cggcgctctg tcgtttcagt ttgtcgccgc cgccgatcca
     8461 gaaggtattg acacagatca ccgtggtcat gccatttcga tccagaaagc agtaaagccg
     8521 gccgttgccg cccgaaatct ggataatccg ccctgtcccg ccaacgcgtt tgatcgtcga
     8581 cagtcggggc agatcatcgt actcggcgat catctggagc ttcttgagaa cccgtttgat
     8641 ttccctcggg ctgctcttga accaggccgc caactcaatc agcacgtcgc agcgcggacc
     8701 gatctgtagc aggcggatcg ccttgcgtcg tccaccttcc tgacccaacc ccttgattgt
     8761 acgcaaaagg agaggagtgt caaccttcag gttaacaccg gctcgccgtt caggcttatt
     8821 cttctcggcg ctcagcgttc cagactcttg ggcggtcggg ggccgcccgt cggctatcgc
     8881 cggctcagcg gcaggaagta atcctctttc agatcctcga gcatccgggc caccacgtcg
     8941 tcgatcacgg ccgactgcga ggccagttgc gtccggacat catagcgttc ggaccactcc
     9001 acccggccgg tctgcacgtc gatcacctgc gcctcgattt ccgccacgct ccaccaccag
     9061 tggatcgtcc ggttttcggc cagatactgg cggatgatcc gccccgtcag gatcttgtcc
     9121 gcgcccagtt ccttgccgta atcgagcgga tcgacctgcc ccagcagatc ctggatctcc
     9181 ccttcggaca gatgcggata ggtatcctcc aggatctggc gcttgtcggc gaagtaatag
     9241 cgcaggtcga tcgccgagta gaggttgatg aaatcatact cggccatcgc gcggttgagc
     9301 gaatcggcga tcatctggcc ggcgcgcggg ttctgccgca ccgtgcgcgc gccgaaccac
     9361 cagccgcgga tcgcgcgcgg cgtcgtgctc atcgaggggg gctgctcaaa cgggaggatc
     9421 agccatttca tcttggggat gcgttcggcc ggaaaccgcg cctggcagcc gccaagcgcg
     9481 ctcaacggca gcaggcaagc gagggtcaag agggcggaaa tcgaacgccg ccacgcgcca
     9541 cgcgcagggg aaatcatgcg ttaagaagcc tccagggagc agccggcgat cccagaccgc
     9601 caaatgtcag accagcttat ccccagcaca aggggtgtca atggaaatgc atgatccagc
     9661 agtgttcgac ccttgtccca cgtccaatgc ctccgacatt cgccttgtcc gcggcgccgg
     9721 gctctcgtac gatccatcct cgggttcatt tccggtgcga tcccgcgcgc gccggcatcc
     9781 cggccagtcc ggtcaccatg cacgccccac tcaccccggt tcccctgcgt cccgccgagc
     9841 gcggtgtcct ggccgtcgtg ctggccggtg ccctgctcct gcgcgcgctg gacgccctcc
     9901 tgtggcatcc gttcacgccc gacgaggaat ttaccctcgc cctgggccgc atgcactggg
     9961 gcgagatgct gcgcgcgacg gcggccgaca cgcaccctcc gctctactat gccctggtca
    10021 agctcgtctt cctgctgacg ccggatgaat acgccccggc gcggctcctc tcggtcgccc
    10081 tcgccggggc gacgctgtgg ctgatctttc gcttcgcgcg cgacggcttt tcccccgcgg
    10141 ccggctgggt cgcgctcctc tgcgcgtcgt tcgcgcccta catgatctac tggcagcacg
    10201 cggcgcggaa ccaccagctc ctgccccccg ccgtcctgct gatcatcggg ctgagctatc
    10261 gctatgcgag cggcggcggc cgcgcgcgct ggtgggcgct ggccgccgcc tggctggtcg
    10321 cgatccagac caactacatg gccctcgtct tcggcctcgt ctggggcgcg gcgttcatcc
    10381 tcgccgagcc gatggcgccg cgccgccgcg cccggctcgc cgcgacgccg ctccccggcc
    10441 tgctttcctt tgttccgtgg ttggggattc tggggcggca ggtccaggcc ggcccgatga
    10501 acaaggggtt ctttcaggaa accgtcagcc cgatctacct ctatttccac gccctgttcg
    10561 gccggatgga gccgtatcag cccaaccaga ccggcccgct gtttctgctc gcgctgctcg
    10621 cgttcgcgct cgtcgcggtg ctgggggcgc gggcggtcgg ccaccgctgg agcttctggg
    10681 ttctgctgct cggcttgccg acgatgccga tcgtggcggc gaaggcggcc ggctggacgc
    10741 tcgccgagcg ccatctcgcc ttcgccctgc cggtgttctt cgtctactgg ggcgcggcgt
    10801 gcgttgcgac agtccggcaa cttcacgccc gatgccgggc gccgggcgtt caacaaccca
    10861 cgggggagga aaccgcgcca tgcagcgcag actcgaagcc gaagtgatgg ataccgaggc
    10921 cgaggcggtc gactacgacc ggatggacca cagcgagccg aacgaggcgt ttgtcgcgcg
    10981 gctgatcgag ctgggcgcct gcggccggat gctcgacatc ggcaccggac ccggccatat
    11041 cccgctcctc gtctgcgagc gcatccgcga tgccgaggtg ctgggcgtcg atctggccga
    11101 cgccatgctg gccatcgccg agcggcggcg gcgggcgtcg gcccaccgcg agcgcctgcg
    11161 ctacgaacgc gccgatgcct gcgcgctgcc gtatgccgac ggcgcgttcg atgcggtttt
    11221 cagcaacacg atcctgcacc aca
//
LOCUS       DAOPCO010000119        11231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5684_length_11231_cov_4.135379, whole genome shotgun sequence.
ACCESSION   DAOPCO010000119 DAOPCO010000000
VERSION     DAOPCO010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5684_length_11231_cov_4.135379"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1621
                     /locus_tag="PLS90_09575"
     CDS             158..1621
                     /locus_tag="PLS90_09575"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HPK02693.1"
                     /translation="MKKTWPFLFALILNSAAGLLAGPRPAAAPAWPATFEDWVVQRVG
                     PVIYPTAPRPKTDWDPLIDLNHELGARAVKTWLGGAAPGEAFARLQTPPYHRLAREHD
                     VIHINVCLNYVTQAYIDNGNRLDNQTLDAAREEWRRITRHLCELTTDPQRIFLLSVGA
                     ELNVYLGTAQRHPDFPVDQLVNTAHQGKEQALAELNGRPRPQVYTVAEFQGEVEFEAY
                     ARKWAPRFETDLISLSYYTFFRPLDDSLTFLRSITPARGPFGADRLMLGEYGLTHEVC
                     NWNQAAHARWHLDMLRQAFDQHAQFAFYYSLADDPGVIRDAGHDGLIGWTRRDDPGRR
                     LTWFLYHALYHRLPPLIPDYELYEYHDELNQPAAGDAPNFVLEDLTVLGAEHRPGDAV
                     AFRVTVRNAGPAPAPAASVNFYVNDRIVAYAGVPALEPGAQAKVESTATDPRFVWSAQ
                     AGTHRLTAHANVRHGVPELAFTDNEATIYFTIAADEP"
     gene            1711..2811
                     /locus_tag="PLS90_09580"
     CDS             1711..2811
                     /locus_tag="PLS90_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK02694.1"
                     /translation="MNSRERVRLAVQHQETDRLPIDFIARDEIAQALAAHFHAPDGEA
                     LLQRLNVDLRPVGPTIKQQASPICYADPTIAVRHDDQLQSDVFYDIWGVGFVRKYALG
                     GEYLDLVHSPLRDKGSLDDLAAYPFPTADIWDYDTIADQTARHGDYCLWAHSRGFFEI
                     SWFMRGMDQFMLDLNLNREYAETLMDRIIDYLLDRTVRILEADRNRRIDIVEINDDVG
                     GQHGLLLSPELWREFIRPRMDRMIRRIKQFGVLIRYHSCGGIREIIPDLIELGVDILN
                     PVQPLARGMELDGLKRDFGARITFDGGVDTQRLLPHRHADEFEAQTRDILRMMGRDGG
                     FIMAPSHALQTDVPLENILKLYELSALPLDRI"
     gene            complement(2874..4295)
                     /gene="carB"
                     /locus_tag="PLS90_09585"
                     /pseudo
     CDS             complement(2874..4295)
                     /gene="carB"
                     /locus_tag="PLS90_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
     gene            complement(4368..4727)
                     /locus_tag="PLS90_09590"
     CDS             complement(4368..4727)
                     /locus_tag="PLS90_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02695.1"
                     /translation="MNPTTLEQTKRAMIEQEPHWYVAFMDFVDEFRRTRDVARFAEPW
                     PLDHPRYDALLASTLEYLCDEAGIEPPAWSWEIPACPDPWFVAGVENLKALALVQSPA
                     HFRRRKIFVLENFLARV"
     gene            complement(4708..5223)
                     /locus_tag="PLS90_09595"
     CDS             complement(4708..5223)
                     /locus_tag="PLS90_09595"
                     /inference="COORDINATES: protein motif:HMM:NF039330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HPK02696.1"
                     /translation="MNKEEIVGALETLNALLRERGIKGEICLYGGAAMCLAFDARQST
                     KDVDAIFHPSTEVRAAAAETARRLGLPVDWLNDGVKEFLVDHPRRAWKSMSNLTVLVA
                     EADYLFAMKAMSARVDTSDRDDIRRLLTELGVLQLDDALTIVEKYYPRNQIKPATLFF
                     LEELFDESDNP"
     gene            complement(5302..6426)
                     /gene="carA"
                     /locus_tag="PLS90_09600"
     CDS             complement(5302..6426)
                     /gene="carA"
                     /locus_tag="PLS90_09600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPK02697.1"
                     /translation="MIHRRPPAVLMLADGTRFDGRAFGAQGESSGEVVFNTAMTGYQE
                     ILTDPSYRGQMVTLTYPLIGNYGTTSFDEQSDRIQAAALIIKELSGVESNFRSQERIE
                     TYLSEAGVIGIEGIDTRELVLKLRTAGAMNGVLSTTDFDDDSLLAKARRAPDMNGLDL
                     VREVTCAQPYRFEPREGYPVVDGPRYRVVAYDFGVKRNILELLCAQNMEVHVVPAGTP
                     PADVLALEPDGIFASNGPGDPAAVTYAIDSLRELIGRKPIFGICLGQQLLGAALGART
                     FKLKFGHRGANHPVRDERTGRIEITSQNHGFCVDPKTLPADCEITHWNLNDNTLEGFR
                     HTKLPLFAVQYHPEASPGPHDSGYLFPRFRALIEEWTGRG"
     gene            6659..6733
                     /locus_tag="PLS90_09605"
     tRNA            6659..6733
                     /locus_tag="PLS90_09605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6691..6693,aa:Gln,seq:ttg)
     gene            6809..7846
                     /locus_tag="PLS90_09610"
     CDS             6809..7846
                     /locus_tag="PLS90_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPK02698.1"
                     /translation="MTPTDRPLAAVIIAAGQGKRMRSRLPKVLHEIAGRPMIDYPLGA
                     VEAVGVERIFVVVGNGGDLVRAHVGGRALCVDQPERLGTGHAVKMAAPHLGDFHGDVL
                     ILYGDTPLLMRETLQRLIDTHRERRGAGVLLAATLRDPTGYGRIHRDANGAVLRIIEE
                     KDCTPEQRLIREANVGAYCFDCRRLLEGLDLLQNNNAQGEYYLTDVPEKLRALGHAVY
                     PVQASDESEVLGVNSRAQLAEVAVYLRRRVLNRLMDEGVTIVDPATTYIDQTVAIGPD
                     TVVEPFTILRGDTTIGERCRIGPGAELIDARVGHGVHIRHSVVHETEIPDGARVGPFA
                     WLRPQQAPQVT"
     gene            8215..11094
                     /locus_tag="PLS90_09615"
     CDS             8215..11094
                     /locus_tag="PLS90_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPK02699.1"
                     /translation="MTWLCFLLTAGASQPSYLHAQNDQPVYRIVFSENVAQLQDAQRL
                     ESMLRDLGYSPLFVEPQGEGFRVLYGHFLTERQANEAMRRLEAEGLLPQGITQVRQSV
                     FAPTTSSAASHTILIRFIDRERAAEAVRRLEASGFSPVSMATVSGQYLVQVGRYTYRD
                     GITNLGRIKGMDYATAELRRLSEQGEALPPRPISETRPPEDSIESVIADRLASSEDFA
                     DLSDEQKDQLVKSIVLTQNLRAGNELAPQVIDMDKRLQSLGEEVEGLLHQVEAEADEA
                     RQIKEQIEELVVQAKRHQAAEEWAEAIQIYQRIRDEVDPQDKLRYRLYIDDQIAWLRG
                     RQSGELIPGQQAETRRQVELLKERALRLAGSADQESLIQAAVLWNQIWSMSPADRQTA
                     EDALGSINASLANIRTQNQELMASTVNKRLQTLTMAGGAALAALLALLAFVWLRGRSQ
                     HKELMRKVQEITSIRPMRELDSGGGGLLDSGGGTATESEIFSPRSPAAAAGPASLDPL
                     GGVEDAPSAGGGSADDDIFGDLSGGLPSAAPANNAAGGATTGVDDMNLDDIFGDVGGA
                     APEATQRIQAPDTAQSQPGGDLDDVFGDIFSAEETSPAVAPGVPETPPPPPQSDTSQP
                     GPISFGDFEFESPGASAEETAAGQSQDDELLSIFDDAIDSATGDETSPSFEPDQGGAE
                     EEAPFAGGLGSNPFTGAPQAVPTDEDNEIPALRLDGGAADDTRDGETSVNFTGFDAPS
                     PENDETNVPIGTMPDFSFDDLSGATAALGQAPGADLDFENDDLGATPLDWTGNHPAAA
                     LTVECESPPRGSHQYLRYEKTEGAAVTPFMHRFGEVGGQVEIEFDLRCNSKNKFLLGF
                     FVEKDGDPRSAIHTKILRSETQTTPTIHMQGEPAPYLLGSWAHIRYVVDLDNGTLNGY
                     IDGTHVARNVTLENNPGSLNTLTIRDNTSTTGVLLLDNIRVQPIS"
BASE COUNT         1960 a         4048 c         3248 g         1975 t
ORIGIN      
        1 agaattggct ccctcggctt ccggattctc attgacatcc gactcactcc tggccgaaaa
       61 cagagcgtca tcgaatgggc gcccattcag ccgatcctct taatctatgt cctcgtttta
      121 ctctgccgcc gccatgtatc acacacgagt gcaaaacatg aaaaaaacct ggcctttcct
      181 cttcgccctt atccttaata gcgcggcggg cctgctcgcc ggcccgcggc ccgcggccgc
      241 tccggcctgg cccgccacct ttgaagactg ggtcgtccaa cgcgtcggtc cggtcatcta
      301 tcccacggcg ccccgcccta agacggactg ggatccactc atcgatttga accacgaact
      361 gggcgcccgt gcggtcaaaa catggcttgg cggcgccgcg ccgggcgagg ccttcgcccg
      421 actccaaacc ccgccctacc accggttggc gcgcgaacat gacgtcatcc acatcaatgt
      481 ctgcctgaac tatgtcaccc aggcctacat cgacaatggc aaccgccttg acaaccaaac
      541 cctggacgcc gcacgggaag agtggcgccg catcacgcgc catctctgcg agctcaccac
      601 cgacccccag cgcatatttc tcctcagcgt cggcgccgaa ctgaacgtct atctcggcac
      661 ggcccagcgc caccccgact ttccggtcga tcaactcgtc aacaccgccc atcagggcaa
      721 ggagcaggcc ctggccgagc tgaacggtcg gccgcggccg caggtctata ccgtggctga
      781 gttccagggc gaggtggaat tcgaggccta cgcgcgcaag tgggcgccgc gcttcgaaac
      841 cgacctgatc agcctctcct attacacttt cttccggccc ttggatgact cgctgacctt
      901 tctccgctcc attacccccg cgcgcggccc cttcggcgcc gaccgcctga tgctcggcga
      961 atatggcctg acgcacgaag tctgcaactg gaaccaggcg gcccatgccc gctggcacct
     1021 ggacatgctg cgccaggctt tcgaccagca tgcccagttt gccttttact attcacttgc
     1081 cgacgacccc ggcgtcattc gcgacgcagg ccacgacggc ttgatcggct ggacccgccg
     1141 cgatgacccc ggccggcgcc tgacctggtt cctctatcac gcgctgtatc accgcctgcc
     1201 gccgctaatc cccgactatg agctctacga gtaccacgat gagctgaacc agccggccgc
     1261 gggcgacgcc ccgaatttcg tgctggagga tctgacggtg ctcggcgcgg agcaccgccc
     1321 gggcgacgcc gtcgcgttcc gcgtcacggt gcgcaacgcc ggcccggcgc ccgcgcccgc
     1381 cgcctcggtc aatttctacg tcaatgaccg cattgtcgcc tatgccggcg tgcccgcgct
     1441 ggaacccgga gctcaggcca aagtcgaatc gacggctacc gacccgcgtt tcgtctggtc
     1501 cgctcaggcc ggcacgcacc ggctcactgc ccatgccaac gtgcgccacg gcgtgcccga
     1561 actcgccttc accgacaatg aagcaacaat ttacttcacg atcgccgcgg acgaaccgta
     1621 gcccgcgccc cgccgcgcgc tgcccgcgcc ggcgttgaac cgcctttgcg cagacctgcc
     1681 aattccatct cgaacagggg gaagtgaacc atgaactcca gggaacgcgt tcggttggcc
     1741 gttcagcacc aggaaacgga ccgcctgccg attgacttca tcgctcgcga cgagattgcc
     1801 caggcgcttg ccgcgcattt tcacgccccc gatggggaag cgctcctcca acgcctcaac
     1861 gtcgatctgc ggcccgtcgg cccaaccatt aagcagcagg cgtcgcccat ctgctacgcc
     1921 gatccgacga tcgccgtgcg ccacgacgac cagttgcaga gcgacgtctt ctacgacatc
     1981 tggggcgtgg gcttcgtgcg caaatacgcg ctcggcggcg aatacctcga cctcgttcac
     2041 agcccgctgc gcgacaaggg cagcctggat gaccttgccg cctacccctt ccccaccgca
     2101 gacatctggg actacgacac gatcgcggat cagaccgccc gccacggcga ctactgcctc
     2161 tgggcgcatt cacgcggctt cttcgaaatc agttggttca tgcgcggcat ggaccagttc
     2221 atgctcgact tgaatctgaa ccgcgaatac gccgaaacgc tgatggaccg gatcatcgat
     2281 tacctgctcg accgcaccgt gcggatcctc gaggccgacc gcaaccgccg catcgacatc
     2341 gtcgagatca atgacgacgt cggcggtcag cacggtctgc tgctcagccc cgaactctgg
     2401 cgcgagttca tccggccccg catggaccgg atgatccgcc ggatcaagca gttcggcgtc
     2461 ctgatccgct atcacagttg cggcggcatc cgcgagatca ttcccgatct gatcgagctg
     2521 ggcgtcgata tcctcaatcc agtgcaaccg ctggcgcgcg gcatggagct cgacgggctc
     2581 aaacgcgact tcggcgcccg catcacgttc gacggcggcg tcgacaccca acgccttctg
     2641 ccccaccgcc acgccgacga gtttgaagct cagacgcgcg acatcctgcg catgatgggc
     2701 cgcgacggag gcttcatcat ggccccctcc catgcgctgc agaccgatgt ccccttggaa
     2761 aacatcctca aactctacga gctgtccgct ctcccgctcg atcggatttg aggtcccgcg
     2821 cggcctccgg tgctgcggac gcgtcaatcc ccaaacgcgc gcgccagccc cgcgagttcc
     2881 tgctcggttt caatcagctc ctcgatgttg cgcagaaacc agggatcgat cccggtcagc
     2941 gcgaaaacct cgtcgcgcga catccccccg ggccccaggg cgtatttcac atagaagatc
     3001 cgctccggat tcggcgtctt gagccgatac gccaactggt ccggatcgat ccggtccttg
     3061 ccgtcggccc ccaggcccgc ccggctgatt tccagcccgc gcagcccctt ctggagcgct
     3121 tccttgaacg tccgcccgat cgccatcgtc tcgccgaccg acttcatctg cgtccccagg
     3181 atcggctcgg cccccggaaa cttctcgaac gccagccgcg ggattttcac cacgcagtag
     3241 tcgatcgtcg gctcgaagca ggccggcgtc ttcagcgtga tgtcgttggc cagttcgtcc
     3301 agccgatacc ccaccgccag cttggccgcg atcttggcga tcggaaaccc cgtcgccttg
     3361 gacgccagcg ccgacgatcg cgagacgcgc gggttcatct cgatcacgat ccgccggccc
     3421 gtcctcggat cgaccgcgaa ctggatgttc gagccgcccg tgtcgacgcc gatcgcccgg
     3481 ataatcgtct tggcgtcgtc gcgcatcgcc tggtactccc ggtcggtcag cgtctggatc
     3541 ggcgccaccg tgatcgagtc gcccgtgtga acccccatcg gatcgaaatt ctcgatcgaa
     3601 cagatgatca cgacgttgtc cgcgttgtcg cgcatcactt ccagctcgaa ctctttccag
     3661 cccagcaggc tctcctcgac catcacctcg cacgtgcgcg acagccgcag gccgcgcgcg
     3721 acgatttcct tgtattcctc gatgttgtaa gcggtgccgc cgcccgagcc tcccagcgtg
     3781 tagctcggcc ggatgatcgc cggaaacttg acgtaatcaa tgacctccag cgcctcttcc
     3841 atcgaatgcg cgaaccccga gcgcggcatg tccagcccgt gcgccagcat cgtgttcttg
     3901 aacagctcgc ggtcctcggc gcgcgcgatc gcctcggcgt tcgccccgat cagctcgatc
     3961 cccagccgct cgaacacccc tttctccgcc agcgccatcg ccgtgttcag cgccgtctgc
     4021 cctcccagcg tgggcagcac cgcgtccggt cgctcccgct ccaggatcgt ctccaccacc
     4081 tccggcgtca ccggctccac atacgtccga tcgccgaact ccgggtcggt catgatcgtc
     4141 gccggattgg agttgaccag cacgacttca tagccgtcgg cgcgcagcgc cttgcagccc
     4201 tgcgttccgg aataatcaaa ctcgcaggcc tggccgatca cgatcggccc cgaaccaatg
     4261 atcaggatct tgtggaggtc ggtgcgtttg ggcatgaagt gctctttcca tcttaagtaa
     4321 ggcgaaacgc cggacttgat tccccgcctc tgcctggcgt ccggtgctca cactcgcgcg
     4381 agaaagtttt ccagtacgaa gattttccgg cgtcggaaat gcgccggact ctgcacgagc
     4441 gccagtgcct tgagattctc tacccccgcg acgaaccatg gatcggggca tgccggaatt
     4501 tcccacgacc aggcgggcgg ttcaatcccc gcctcgtcgc aaagatattc cagcgtggac
     4561 gccagcaacg catcgtagcg cggatgatct agcggccagg gctccgcgaa ccgcgccaca
     4621 tcgcgcgtac gacggaactc gtcgacgaaa tccatgaacg ccacgtacca atgcggttcc
     4681 tgctcgatca tcgcgcgctt ggtctgttca agggttgtcg gattcatcga acagttcctc
     4741 gagaaagaac agcgtggccg gcttgatctg attccgcgga taatacttct ccacgattgt
     4801 caacgcgtcg tccaattgaa ggactcccaa ttcggtcaac agccggcgga tgtcgtcgcg
     4861 gtccgaggtg tccactcgag cggacatggc tttcatcgcg aacaggtaat cggcctcggc
     4921 gacgagcacc gtcagattgg acatactttt ccatgccctg cgcgggtggt cgaccagaaa
     4981 ctctttcacg ccgtcattga gccagtcaac gggtaggccc aaccgccgcg cggtctcggc
     5041 ggccgccgcg cgcacctcgg ttgacggatg gaagatcgcg tcgacatcct tcgtgctctg
     5101 gcgcgcatcg aatgccagac acatcgccgc gccgccgtag agacagattt ctcctttgat
     5161 gcccctttcg cgtagaagag cattgagcgt ttccagagcg ccgacaattt cctctttgtt
     5221 catgttgcct tcctcgttga cgccagggcc gccatctcgg cctccgtcgt cgcctgatcc
     5281 tggtcgcggg cgatttgccg atcaccctcg cccggtccac tcctcaatca gcgcccggaa
     5341 ccgcgggaac agatacccgc tgtcgtgcgg cccgggactc gcctcggggt gatactgcac
     5401 ggcgaacagc ggcagcttcg tatgccggaa gccctccagc gtgttgtcgt tgagattcca
     5461 gtgcgtgatc tcgcaatcgg ccggcaacgt cttcgggtcg acgcaaaacc catgattctg
     5521 gctggtgatt tcgatccgcc ccgttcgctc gtcgcgcacc gggtgattgg cgccccggtg
     5581 cccgaacttc agcttgaacg tcctcgcccc cagcgccgcc ccgagcaact gctgtcccag
     5641 acagatgccg aaaatcggct tgcgtccgat cagttcgcgc aggctgtcga tcgcgtaggt
     5701 caccgccgcc gggtcgcccg gcccgttgct ggcgaagatc ccgtccggct ccagcgccag
     5761 tacgtcggcg ggcggcgtcc ccgccggcac gacatgcacc tccatgtttt gcgcgcacag
     5821 cagctccagg atgttccgct tcacgccgaa gtcatacgcc actacccggt agcgcggccc
     5881 gtccaccacc ggatacccct cgcgcggctc aaaccggtac ggctgggcgc acgtcacctc
     5941 ccgcaccagg tccaggccgt tcatgtcggg cgcccgacgc gccttggcca gcagcgagtc
     6001 gtcatcgaag tccgtcgtcg agagcacgcc gttcatcgcc cctgccgtcc gcaacttcag
     6061 cacgagttcc cgcgtgtcga tcccctcgat gccgatcacg ccggcttcgc tcagatacgt
     6121 ttcgatccgt tcctgcgagc ggaagttgga ctccacgccc gaaagctcct tgatgatcag
     6181 cgccgccgcc tggatccggt ccgactgctc gtcgaacgag gtggttccgt agttgccgat
     6241 caacggatat gtcaacgtca ccatctggcc gcgatagctc ggatcggtca ggatctcctg
     6301 atagccggtc atcgccgtat tgaacaccac ttcgccggag ctctcgccct gggcgccgaa
     6361 ggcgcgcccg tcgaagcggg tgccatcggc cagcatcagg acggcggggg ggcggcggtg
     6421 gatcatcggg catccttttt gaggcgggct tcgcgcacca tccggctcca cccggttggc
     6481 acgccgcgcg atgcgcgcaa caggtaaaat cccaatattc tcccgtgccc tcacccggcc
     6541 tgtcaagccc cgccctctct tcttgccttc cccgtctttc cgtcttcgcc atctccccgc
     6601 ctcccgcaat tgacacccac tccccatccc gataagaata gaaaggccga cccgacgcag
     6661 ggggttcgcc aagtggtaag gcaccggatt ttggttccgg cattcgaagg ttcgaatcct
     6721 tcaccccctg ccaatccatc cgcgaccctc aatctccgcg tggaagccgc tccatccggc
     6781 gcaaccctcc cttgaaattc ccttgccgat gacaccaacc gaccgccccc tcgccgccgt
     6841 cattattgcc gcggggcagg gcaaacgcat gcgctccagg ctccccaaag tgctccacga
     6901 gatcgcgggg cggccgatga ttgactaccc cctgggcgct gtcgaagcgg tcggcgtcga
     6961 acgcatcttc gtcgtcgtgg gcaatggcgg cgacctggtg cgcgcgcacg tcggcgggcg
     7021 cgccctctgc gtcgaccagc ccgaacgcct cggcaccggc catgccgtca agatggccgc
     7081 gccccatcta ggcgactttc acggcgacgt cctgattctc tacggcgata cgccgttgtt
     7141 gatgcgcgag acgctccagc gcttgatcga cacgcaccgc gagcggcgcg gcgccggcgt
     7201 cctgctggcg gccacgctgc gcgaccccac cggttacgga cgcatccacc gcgacgcgaa
     7261 cggcgccgtc ctgcgcatca tcgaggagaa ggactgcacg ccggaacagc gcctgatccg
     7321 cgaggcgaat gtcggcgcct actgtttcga ctgccgccgc ctgctcgagg gtctcgacct
     7381 gctccagaac aacaatgcgc agggggagta ttacctgacc gatgtccccg agaaactgcg
     7441 cgcgctgggt cacgcggttt atcccgtgca ggcctcggac gaaagcgagg tgctgggggt
     7501 caactcccgc gcccagttgg ccgaggtcgc cgtctatctg cgccgccgcg tgctgaaccg
     7561 gctgatggat gagggggtca cgatcgtcga tcccgcgacg acttacatcg atcaaaccgt
     7621 cgcgatcggc cccgacacag tcgtggaacc gtttaccatc ctgcgcggcg acaccaccat
     7681 tggcgaacgc tgccgcatcg ggccgggcgc cgagctgatc gacgcgcgcg tcggccatgg
     7741 ggtccacatc cgccacagcg tcgtgcacga gacggagatc cccgatggcg cgcgggtcgg
     7801 cccgtttgcc tggctgcgac cccagcaggc cccgcaagtg acttaagcga cttgggttgg
     7861 acggattcgc gcttgatgaa cccatcgttt tgtgtcttga tgttgactga gtgatcccac
     7921 cggcgtcgcg ggatggggat ccgtcatttg caccccctgt cggcggcgga gttccggtcg
     7981 ggcgggccct ccggccgaac gcggacgtcg gatgccgtct cgcgcgcgcc ggcgggcggg
     8041 ttggggcacc cgattctccg ccccgcctct ttgcctccgg ggggggtggc ttgcggggtc
     8101 gcgggacacc taaatggcag attacgaact ctcaagaagc aggtgaggcc gcgcatcgcc
     8161 gtcggcccga gaaagggaat gtctatgatg tgccgggcga ttgggctccg gtctttgact
     8221 tggctgtgct tcctcctgac ggccggggcc tcccaaccct cctacctcca cgctcagaac
     8281 gaccagccgg tctaccggat cgtcttcagt gaaaacgtcg cgcagttgca ggatgcccag
     8341 cgcctcgaat cgatgctgcg ggacctgggt tattctcccc tcttcgtcga accgcaaggc
     8401 gagggcttcc gtgtcctcta tggtcacttc ctcaccgaac ggcaggcgaa tgaggccatg
     8461 cgccgtctgg aggctgaggg cctgctcccc cagggcatca cgcaggtgcg ccagtcggtt
     8521 ttcgccccga ccaccagttc cgcggccagt cacaccatcc tgattcgttt catcgaccgc
     8581 gagcgcgccg ccgaggcggt gcgccggctc gaagccagcg gcttcagccc cgtcagcatg
     8641 gcaaccgtct cgggccagta cctcgttcag gtcggccgct acacttaccg cgacggcatc
     8701 accaacctcg gccggatcaa gggcatggac tacgccacgg ccgaactgcg ccggctttcc
     8761 gaacagggcg aagccctgcc cccccgcccg atctccgaaa cccggccgcc ggaggacagc
     8821 attgaatcgg tgatcgccga tcgcctcgcc agttccgagg atttcgccga tctgagcgac
     8881 gaacagaagg atcaactcgt caaaagcatc gtgctgaccc agaacctgcg ggcgggcaat
     8941 gagctggccc cgcaggtcat cgacatggac aagcgtctcc agtcgctcgg cgaggaggtc
     9001 gagggcctgc tgcaccaggt cgaggccgag gccgatgagg cccgccagat caaggagcag
     9061 atcgaggaac tcgtcgtcca ggccaagcgc catcaggcgg ccgaggagtg ggccgaggcg
     9121 atccagatct atcaacggat ccgcgacgag gtcgatcccc aggacaagct gcgctaccgc
     9181 ctctacatcg atgaccagat cgcttggctg cgcggacggc aatccggcga actgatcccc
     9241 ggccaacagg ccgagacccg gcgccaggtt gaactgctca aggaacgcgc gctgcgcctg
     9301 gccggctcgg ccgatcagga gtcgctgatc caggccgccg tcctctggaa tcagatctgg
     9361 agcatgagcc ccgccgatcg ccagacggcc gaggatgccc tcggcagcat caacgcctca
     9421 ctggccaaca tccgcaccca gaaccaggag ctgatggctt ccacggtgaa caagcgtctg
     9481 cagacgctga ccatggccgg cggcgccgcc ctggccgccc tgctcgcgct gctcgcgttt
     9541 gtctggttgc gcggacgctc gcaacacaag gagctgatgc gcaaggttca ggaaatcact
     9601 tcgatccggc ccatgcgcga attggacagc ggcggcggcg gattgctcga cagcggcggc
     9661 ggcaccgcca ccgagtcgga gattttctcc ccgcgttccc cggcggcggc cgccggcccg
     9721 gcctcgctcg atccgctcgg cggcgtcgag gacgccccct cggccggcgg cggctcggcc
     9781 gatgacgaca tcttcggcga tctgagcggc ggcctgccca gcgcggcgcc ggcgaacaac
     9841 gccgccggcg gcgcgacgac tggcgtcgac gacatgaatc tggatgacat cttcggcgat
     9901 gtcggcggcg ccgctcccga agccacgcag cgcatccagg cccccgacac ggcccagagt
     9961 caacccggcg gcgatctcga cgatgtcttc ggcgacatct tctcggccga ggagacctcg
    10021 ccggcggtgg cacctggcgt tcccgaaacg ccccccccgc cacctcagtc cgatacgtct
    10081 caacccggac cgatctcgtt cggcgacttc gagtttgaat cgccgggcgc ttcggccgag
    10141 gagaccgccg ccggccagag ccaggacgat gaactcctct cgatcttcga cgatgccatc
    10201 gattccgcta ctggcgacga aactagccca tccttcgaac cggatcaagg aggggcggaa
    10261 gaagaagccc ctttcgccgg gggcctgggg agtaacccgt tcacgggtgc gcctcaggcc
    10321 gttccgaccg acgaagacaa cgagatcccc gcgctgcggc tcgatggcgg cgcggccgac
    10381 gatacccgcg acggcgaaac ctcagtcaat ttcaccggat ttgacgcgcc ttcccccgag
    10441 aacgacgaaa ccaacgtccc catcggcacg atgcccgact tctcgttcga tgatctctcc
    10501 ggcgccaccg ccgcgctcgg acaggccccg ggcgccgatc tcgatttcga gaacgacgac
    10561 ctcggcgcca cgccgctcga ctggaccggc aatcaccccg ccgcggcgct gaccgtcgaa
    10621 tgtgaaagcc cgccccgcgg ctcccaccag tacctccgct acgagaaaac cgaaggcgcc
    10681 gccgtcaccc ccttcatgca tcgcttcggc gaggtcggcg gtcaggtcga gatcgaattc
    10741 gacttgcgct gcaacagcaa gaataagttc ctcctcggct tcttcgtcga aaaggatggg
    10801 gacccccgtt cggcgatcca caccaaaatc ctgcgtagtg aaacacaaac cacgccgacg
    10861 attcacatgc agggcgaacc ggccccctac ctgctcggct cctgggccca catccgctac
    10921 gtcgtcgatt tggataacgg aactctcaac ggctacatcg acgggacgca cgtcgcgcgc
    10981 aacgtcaccc tcgagaacaa tcccggttcg ctcaataccc tgacgatccg cgacaacacc
    11041 agcaccaccg gcgtcctgct gctcgacaac atccgggtac agccgatttc gtgaaaatcg
    11101 cctggcgtca ccgctggaac gtaacccccg ccgaagcacg tcagctccag gccgaggcgc
    11161 gccaccgggt catcgttcgc gacgactccc ccgtgttgac cttcccccgg cgcgtcgtcg
    11221 cgcttgacgt c
//
LOCUS       DAOPCO010000120        11133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5735_length_11133_cov_5.469670, whole genome shotgun sequence.
ACCESSION   DAOPCO010000120 DAOPCO010000000
VERSION     DAOPCO010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5735_length_11133_cov_5.469670"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..678)
                     /locus_tag="PLS90_09620"
     CDS             complement(196..678)
                     /locus_tag="PLS90_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK02700.1"
                     /translation="MTADALDAIEFRPADLGEILELREHELIRGTGREREFPGDRDPT
                     TRHFGAFRRGTCVGCLSAMLSEWEGAPAWQVRGMAVAADWRGRGVGARLLARAGALLV
                     EERAGPGGTRVRLLWCNARRPAVGFYEKQGWRVVSGPFEIPSVGPHVRMVKGIGEAEV
                     "
     gene            complement(702..2330)
                     /locus_tag="PLS90_09625"
     CDS             complement(702..2330)
                     /locus_tag="PLS90_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02701.1"
                     /translation="MSASTEKIRFLHLLLTLAQLPAFAPASWAQETFDLEDYFILTQG
                     SRWHYTAINGASTDDDFAWEVLPETITVTGGHEATRIYTDVDDPGDSRMDDEDFWYFD
                     SNGDLYFAGFHNGVADGGGTFPAQTIILTDPIKIGGRDMEIGEVVMDTGAGTVQTALG
                     PINATIQSTITYIEVMDQVVTPLGNFKNAIRLHIEISGSAGFVNFDIKDADVILARHV
                     GMILQSQEFDQDDAEAHAIDGGFIAGMTITDDPPCTNFLNPMMVGTIDVQDLEDPSGL
                     AASTKNPGVLWTHDDDGAAPNLYAISTTGAHLGTFTITGLAPGDWEDLAVGPGPDAQK
                     TYLYIADIGDNAETRAQINIYRVEEPAVSSSQAPPLMRNIASFENFPLVFPDAPDNAE
                     AFLLDPTTLDFYIIVKKGDGKSRVWKAAAPHTHNQQATLELIDTLYFDNQVTGGAVNQ
                     DGSQFAVRTSTEIFTFTIPAGKTMQEVITGFTYQPCVVSTMTEDDGEAVAFNPNGGFY
                     TTQEVSANKAGGQPLFFYPDLASNAVGGSWTTYR"
     gene            2666..4147
                     /locus_tag="PLS90_09630"
     CDS             2666..4147
                     /locus_tag="PLS90_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK02702.1"
                     /translation="MSTAAAHPPSSSGAVRRDALLLIVDDREDNRVTIAEAFERQGYA
                     VLTAAGGQEALRQIAEHAPDLVICDLKMPDMDGLEVLRTARAAQNPPEIILLTAYGTV
                     ETAVDALKLGAFNYLTKPVNLKDLRAQAEKALEHRQLRRDVGRYRRQAEAAAHNGWPE
                     QFVFESPAMRRLMEMVRRLAPTQSNVLIDGESGVGKEVVAQALHNLGPDPAARPFIVV
                     HCAALPESLLEAELFGHEKGAFTGAEKQTLGRLELADGGTLFLDEIGEVPLAMQVKLL
                     RVLEQRELMRVGGRRVIRVNFRLLAATNRNLEEEVAAGRFREDLFYRLNVVRLRVPPL
                     RERRDDILPLLRHFLTRFAAEGGQPQPRLTPEVERRLVAYPWPGNVRELRNLAERLSI
                     FAAGRELRADDLPFEIANPFAAGRIVAGRGLGPGSAVATDMLDLQQIERITIERALAR
                     FGGNRSAAAEALGISRRTLQRKLKEYQMAAQEDEDDSTDENGD"
     gene            4340..4888
                     /locus_tag="PLS90_09635"
     CDS             4340..4888
                     /locus_tag="PLS90_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HPK02703.1"
                     /translation="MNHPWHDVPLGAESIDNMQAVVEIPKGSKVKYELDKTTGLLRVD
                     RVLYSSVTYPANYGFLPQTLGDDHDPLDVLVLMQEPVISLSILRVKPIGMMTMIDQGE
                     NDEKIISVHLDDPEYRDYDHIRQLPPHRLMELRMFFEDYKKLEHKEVRVRDFFGPDDA
                     RRVVAHCARQYQEQVAPTRGVA"
     gene            complement(4905..5651)
                     /locus_tag="PLS90_09640"
     CDS             complement(4905..5651)
                     /locus_tag="PLS90_09640"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HPK02704.1"
                     /translation="MKALAACGTRVMTAGLMLIAALGARVGAEEIPAVAEAPTTVTVA
                     VLTGGHGYDEPAFEILFDSLAGVEATIVNERDFLTAPAEERDGYDVVLFYNMIMETPE
                     GDYKEGLEHLGTSDQGIVVMHHALLSYPEWPGWTDVMGIADRKFGYFHDQQVKVHVEE
                     VEHPITAGIQDWEMIDETYTMADADPADGNTVLLTLDQENSMRTIGWTREHGQARVFC
                     LQSGHDNQTWVDPNFREVLRRGILWAAGRL"
     gene            complement(5706..7040)
                     /locus_tag="PLS90_09645"
     CDS             complement(5706..7040)
                     /locus_tag="PLS90_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02705.1"
                     /translation="MKQGKTGGKKLTRRGFLGRALAAAAAPYIIPGSALGLNGHTAPS
                     NRVVMASIGLGFAWDMFLQHKDVQLVAVCDVQRERRDAAKAKVDAFLQNQDCRTYNDF
                     REVIARPEIDAVYIATPDHWHALVTIAAARAGKHIYCQKPLTRTIAEGQAVVEAVRRH
                     NVVFQHGTQQRHDLAMLFGCELALNGYIGEVKQLKIGSPRGQTCPPQPIEPVPDGLDW
                     EMWVGPAPWSPFTGRRIKSHDWYFISDYSMGYIAGWGVHHADSGQQASGMDEPTGLIE
                     VDARGTFATDGLFDTPYEWNMNFKFPNGITWNWTDTPNWYPERNWSDPVRHNMGIRIE
                     GTEGSIFIWRSQVEAEPKSLLNVKIGHRDKVQLCRPGGEPIPDFIECVRRGLRTCAPV
                     EVAHRSTTLCSLGAISMQLGRKVVWDPVKEEIVNDPEAQRLCSRAMREPWSI"
     gene            complement(7146..9215)
                     /locus_tag="PLS90_09650"
     CDS             complement(7146..9215)
                     /locus_tag="PLS90_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02706.1"
                     /translation="MPAGGWARALLFAMAAWTAMLGAGATADPPAGAEELDAAFAALA
                     GYSWEATREPLTVIDAAIAQSRGDEAARAELETRLMAVLGGDAPQAAKKFACERLSLI
                     GSGRSVAALASLLGVRELAHPARLALERIPDPVVDAALIGALETTDGDLLRGTIGSIG
                     NRGVAGATAPLTQWARDDAASSETVLTAIRALGMIGTAESARALGELQAEAPAELRDA
                     ITDARLIAARRMQRAGLNEEAAALYREVLALEGGERWRAACLRGLVDAEPEHAREHLA
                     EALAGEDARLMRVAAETIADADDPAAVRHFSAMLSDLPRASQVILLDTLRSQLGTEAR
                     DFARGAVRSDAAELRLAAIRALGFVGDETDLAELIRAAQSEVEAERDAARTSLELLPA
                     EATGAQLVAMLPAADPGAQAEIIAALAARREVDSLGTITDYLDAENNAVRAAGWEALA
                     TMGTLEQLPVVIEHLQRAEGNEAPKPVREALQAIVRREREAALPTLLSSLSEAPVAAR
                     VALLHSLALLGTGEALEAVRAAVGESDPALKDGAVRTLTEWPTVEAAPDLLRIAREGE
                     KPVWRILALRSYIQLVRGSDATREEKLEQLRPAIELAVNAEEKRRVISALGELAIEPT
                     LAVLSDYMDDPDVVEEASAAVIGVAGPLRKEHREEVDRLLNLVLERSKNPDHHQWTNW
                     LLHHQEE"
     gene            9675..10814
                     /locus_tag="PLS90_09655"
     CDS             9675..10814
                     /locus_tag="PLS90_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPK02707.1"
                     /translation="MNPPDPPANPPHPSPEAPRPTRPAPSRGHRFGWRARTALLLGAI
                     ALPVLLVEFGLRVVGYSHPLYPEKVQFGSPTRAEIDLYGGWDEDPTRFWLPTDYRARL
                     QQVGRPDVAFLGCSCTMYGDYDVHLARMVADRHPGRGFSYINLGVLGWTSHQGLQQFK
                     QDVIPLQPKVATVYFGWNDHWIGFGVEDKEVARLNESLLFKLRNVRLLQLARDAFIKL
                     RKDPDAGYPLRVSLADYRANLREMARLAREHDIQLVLMTAPDGFVPGEEQRYAHLVPG
                     FVASTEKLRNLIPLHEAYVEAVRDVAREEPGVILCDLAAEFDQRFSQEEISERLFTPD
                     AIHLKSVGCQAIAEILLECLEQHGLLEEILRPAAEVTQLPGRGVP"
BASE COUNT         1766 a         4209 c         3175 g         1983 t
ORIGIN      
        1 catgggtctt ctccttgttt gagccgaatt ccgcgacgcc ccggccccct ctcgcggcct
       61 tccggtcgac ggatgcacac ggttcccaac ctaccccacc ccgcccctca attcaacgcc
      121 tttcgggtcc ccaaccccct cgcctcgtcc ctcgtcctcg gctctcgaaa acccgtccac
      181 cccgtccatc ccccgtcaca cctccgcctc gccgatccct ttcaccatcc gcacatgcgg
      241 gcccacggag ggaatctcga acggtcccga gacgacgcgc cacccctgtt tctcgtaaaa
      301 accgaccgcc ggccggcggg cattgcacca cagcaaccgc acccgcgtcc ccccgggccc
      361 ggcccgttcc tcaaccagca gcgctccggc ccgcgccagc agccgcgcgc cgacgccccg
      421 cccgcgccag tcggcggcga cggccatgcc gcggacctgc cacgccggcg ccccttccca
      481 ctcgctcagc atggccgaca ggcacccgac gcacgtcccc cgccggaacg cgccgaaatg
      541 gcgcgtggtc gggtcgcgat caccgggaaa ctcccgctcg cgacccgttc cacgaatcaa
      601 ttcatgttcg cgcaactcga gaatctcgcc gaggtcggcc gggcggaatt cgatggcgtc
      661 gagcgcatcg gcggtcatcg cattccaccc ggccccgcgc ctcagcggta ggtcgtccag
      721 gatccgccga ccgcattgct cgccagatcc ggatagaaga acaacggctg cccgcccgcc
      781 ttgttggccg agacttcctg cgtggtgtag aagccgccgt tgggattgaa cgccaccgcc
      841 tccccatcgt cctcggtcat cgtgctgacg acgcacggct ggtacgtgaa gcccgtgatg
      901 acctcctgca tcgttttgcc cgcggggatg gtgaacgtga agatctccgt gctcgtgcgc
      961 accgcgaatt gcgacccgtc ctgattgacg gcgccgcccg tgacctgatt gtcgaagtac
     1021 agcgtgtcga tcagctcaag cgtcgcctgc tgattgtgcg tgtgcggcgc cgccgccttc
     1081 cacacccgcg acttgccgtc ccccttcttc acgatgatgt agaagtcgag cgtcgtcgga
     1141 tcaagcagga acgcctcggc attgtccggc gcatcgggga acaccagcgg gaagttctcg
     1201 aagctggcga tgttccgcat cagcggcggc gcctggctgg agctgaccgc cggttcctcg
     1261 acgcggtaga tgttgatctg cgcgcgcgtc tcggcgttgt cgccgatgtc ggcaatgtag
     1321 agatacgtct tctgcgcatc cgggccggga ccgaccgcga ggtcctccca gtcgcccggc
     1381 gccaggcccg tgatcgtgaa cgtccccagg tgcgccccgg tggtcgaaat tgcgtaaagg
     1441 ttgggcgccg cgccgtcgtc gtcgtgcgtc cacagcaccc ccgggttttt cgtgctggcc
     1501 gccagtcccg aggggtcttc caggtcctgc acgtcgatcg tcccgaccat catcgggttc
     1561 aggaagttgg tgcagggcgg atcgtcggtg atcgtcatgc cggcaataaa gccgccgtcg
     1621 atcgcgtgcg cctcggcgtc atcctggtcg aattcctgcg actgtagaat catgccgaca
     1681 tggcgggcga ggatcacgtc ggcgtcctta atgtcaaaat tgacgaaccc ggccgaaccg
     1741 cttatctcga tatgcagccg gatcgcgttc ttgaaattgc ccagcggcgt aaccacctgg
     1801 tccatcacct cgatataagt gatggtcgac tggatcgtgg cgttgatcgg tcccagcgcc
     1861 gtctgaaccg tcccggcccc cgtatccatg acgacctcgc cgatttccat gtcgcgcccg
     1921 ccgattttga tcgggtcggt cagaatgatc gtctgtgcgg ggaacgtgcc gccgccgtcg
     1981 gcgacgccgt tatgaaatcc ggcgaaatac aggtcgccgt tggagtcaaa gtaccagaag
     2041 tcttcatcgt ccatccgcga gtcgcccgga tcgtcgacgt cggtgtaaat tcgcgtcgct
     2101 tcgtgaccgc cggtcaccgt gatcgtctcg ggcagcactt cccacgcaaa gtcgtcgtcg
     2161 gtcgaagcgc cgttgatcgc cgtgtaatgc cagcggcttc cctgcgtcag gatgaagtaa
     2221 tcctccaggt cgaacgtctc ttgcgcccag gaggcggggg cgaacgccgg cagctgcgcc
     2281 agcgtcagca gtaagtgtaa aaagcgaatc ttttcagttg aggcagacat gggacttcct
     2341 ttcctgtctg tccggggtgt ccggtgacga gtcgtgacgg ccgggtctgg ccgccctcga
     2401 ttacatgact agcatctcag tttaattatg cgggtttccc gctgcaacta taagatcagc
     2461 ccctcccccc acgaatcatc gcccgcgatg cctgttcagg acatcaggat gatcctctct
     2521 catcacctgc gcagggagcc gccctccggc cgggcactcc ggccggcaat tgatccccca
     2581 cggcggaaca cttgcatttt gccccccctt tctgtccaca atagggcgtc atgcattcgt
     2641 cgctggaccc agcccgctcc cggccatgag caccgccgcc gcccatcccc cctcctcttc
     2701 gggcgcagtg cgtcgcgacg ccctgctgct catcgtcgac gaccgcgagg ataaccgcgt
     2761 gacgatcgcc gaggccttcg agcgccaggg ctacgccgtg ctgaccgccg ccggcggcca
     2821 ggaggccctc cgccagatcg ccgagcatgc cccggacctc gtcatctgcg acctgaaaat
     2881 gcccgatatg gacggcctgg aagtcctcag gacggcgcgc gccgcccaga atccccctga
     2941 aatcatcctg ctcaccgcct acggcactgt cgaaacggcc gtcgatgccc tcaagctggg
     3001 agccttcaat tacctgacca agccggtcaa cctgaaggat ttgcgcgcgc aggccgaaaa
     3061 ggcgctcgag caccgccagc tgcgccgcga cgtcggacgc tatcggcgcc aggccgaggc
     3121 cgccgcccat aacggctggc ccgagcagtt cgtcttcgaa agcccggcca tgcgccgcct
     3181 catggaaatg gtccggcgcc tcgcgccgac ccagtccaac gtcctgatcg acggcgaaag
     3241 cggcgtcggc aaggaagtcg tcgcccaggc cctccacaat ctcgggcccg atcccgccgc
     3301 ccgcccgttc atcgtcgtcc attgcgccgc tttgcccgag tcgctcctcg aagccgaact
     3361 gttcggccac gagaagggcg ctttcaccgg cgccgagaag cagaccctcg gccggctgga
     3421 actggccgac ggcggcaccc tcttcctcga cgagatcggc gaagtccccc tggcgatgca
     3481 ggtcaagctg ctccgcgtcc tggagcagcg cgagctgatg cgcgtcggcg gccgccgcgt
     3541 catccgcgtc aacttccgcc tgctggcggc caccaatcgc aatctggagg aggaggttgc
     3601 cgccggccgc ttccgcgagg acctcttcta ccgcctcaac gtcgtgcgct tgcgcgtccc
     3661 gccgttgcgc gagcggcgcg acgacatcct gccgctgctg cgccatttcc tgacccgctt
     3721 cgccgccgag ggcggccagc cgcagccccg gctgacgccc gaggtcgagc gtcgcctggt
     3781 cgcctaccct tggccgggca acgtgcgcga gctgcgcaat ctggccgagc ggctctcgat
     3841 cttcgccgcg gggcgcgaac tgcgggccga cgatctcccc ttcgagattg ccaacccctt
     3901 cgccgccggg cggatcgtgg ccggccgcgg attgggaccg ggcagcgccg tcgcgacgga
     3961 catgctcgac ctgcagcaga tcgagcggat cacgatcgag cgcgccctcg cccgcttcgg
     4021 cggcaatcgt tccgccgccg ccgaggcgct cggcatcagc cggcgcacgc tccagcgcaa
     4081 actgaaggaa taccagatgg ccgcgcagga ggatgaggac gactccaccg acgagaatgg
     4141 cgattgaaac aatgaggccc tgtctcttac gtcccttatg tccctcccat cacacgaatt
     4201 ttcatccgtt gaataaggga atgacgcgaa ttttttgaat ttttctatta ggatttgttt
     4261 cggattccgg aattcgattt tcgaatttcc ccaccaggac aagggaccga actcctcacc
     4321 aaggacgcgc accccgatga tgaatcaccc gtggcatgac gttccgcttg gcgcggaatc
     4381 gatcgacaac atgcaggccg tcgtcgaaat ccccaagggg agcaaggtca aatacgaact
     4441 ggacaaaacg accggcctcc tgcgcgtcga ccgcgtcctc tattcctcgg tcacctaccc
     4501 cgccaactac gggttcctgc cccagacgct cggcgacgat cacgacccgc tggacgtcct
     4561 cgttctgatg caggagcccg tcatttcgct ctcgatcctc cgcgtcaagc cgatcggcat
     4621 gatgacgatg atcgaccagg gcgagaacga cgaaaaaatc atcagcgtcc acctggacga
     4681 cccggagtat cgcgactacg atcacatccg ccaactgccc ccccatcggc tgatggaact
     4741 gcgcatgttc ttcgaggact acaagaaact ggagcacaag gaagtgcgcg tgcgcgactt
     4801 cttcggcccc gacgacgccc gccgcgtcgt ggcgcattgc gcccgccagt atcaggagca
     4861 ggtcgccccc acccgcggcg tcgcgtgagg ttcaggggct ccggtcacag ccgcccggcc
     4921 gcccacagaa tcccccggcg cagaacctcg cggaaattgg gatccaccca cgtctggttg
     4981 tcgtgacccg attgcaggca gaacacccgc gcctgcccat gttcccgcgt ccagccgatc
     5041 gtccgcatgc tgttttcctg gtccagcgtc agcagcaccg tgttcccgtc ggccgggtcg
     5101 gcgtcggcca tcgtataggt ctcgtcgatc atctcccaat cctggatgcc ggcggtgatt
     5161 ggatgctcga cctcctcgac gtgtactttc acctgctgat cgtgaaagta tccgaacttg
     5221 cggtcggcga tccccatcac gtcggtccag ccgggccact ccggatacga gagcagggca
     5281 tgatgcatca caacgatccc ctggtcgctc gtgcccaggt gctccagacc ctccttgtag
     5341 tcgccttccg gcgtttccat gatcatattg tagaacagga cgacgtcgta accgtcgcgt
     5401 tcctccgccg gcgcggtcag gaaatcgcgc tcgttcacga tcgtcgcctc aaccccggcc
     5461 agtgagtcga acaggatctc gaaggcgggc tcatcatagc cgtggccgcc ggtcaggacc
     5521 gcgaccgtca ccgtggtcgg cgcttccgcc acggccggaa tttcctcggc cccgaccctc
     5581 gcgcccaaag ccgcgatgag catcaggccc gccgtcatca cgcgcgtccc gcatgccgcc
     5641 agtgccttca ttgtgtctcc cctttcgtgt cattcgtgat ccgcaattac ttcttgatcc
     5701 gcctttcaga tgctccacgg ttcgcgcatc gcgcgcgagc acaaccgttg cgcctccgga
     5761 tcattgacga tctcttcctt caccggatcc cagacgactt tacggccgag ctgcatcgag
     5821 atggccccca gcgagcagag cgtcgtcgag cgatgcgcga cttccaccgg cgcgcacgtc
     5881 cgcagccccc gccgcacgca ctcgatgaaa tccgggatcg gttccccccc gggccggcag
     5941 agctggacct tgtcccggtg gccgatcttc acgttcaaca gcgacttggg ttccgcttcc
     6001 acctgcgaac gccagatgaa gatcgaacct tccgttccct cgatccggat ccccatattg
     6061 tgcctgaccg ggtcgctcca gttgcgctcc ggataccagt tcggcgtgtc ggtccagttc
     6121 cacgtgatgc cgttggggaa cttgaagttc atgttccact catacggggt gtcgaacagc
     6181 ccgtcggtcg cgaacgtccc gcgcgcgtcg acctcgatca gccccgtcgg ctcgtccatc
     6241 ccgctggcct gttgcccgct gtcggcgtga tggacgcccc accccgcgat gtaacccatc
     6301 gagtaatccg agatgaagta ccagtcgtgc gacttgatcc gccgcccggt gaacggcgac
     6361 cacggcgccg gcccgaccca catctcccag tccagcccgt ccggcaccgg ctcgatcggc
     6421 tggggcgggc acgtctgccc ccgcgggctg ccgatcttca actgcttcac ctcgccgata
     6481 tacccattga gcgccagttc gcacccgaac agcatcgcca ggtcgtggcg ctgctgcgtt
     6541 ccgtgctgaa acacgacgtt gtgccgccgc accgcctcga cgaccgcctg cccttcggcg
     6601 atcgtccgcg tcagcggctt ctggcaatag atgtgcttgc ccgcgcgcgc cgcggcgatc
     6661 gtcaccagcg cgtgccagtg atcgggcgtc gcgatgtaga ccgcgtcgat ctccggccgc
     6721 gcgatcacct cgcgaaaatc gttgtaggtc cggcagtcct gattctgcag aaacgcgtcg
     6781 accttcgcct tggctgcgtc gcgccgctcg cgctgcacgt cgcacaccgc cacgagctgg
     6841 acatccttgt gctgcaggaa catgtcccag gcaaagccca ggccaatcga cgccatcacc
     6901 acccgattgc tcggcgcggt gtggccgttc agcccgagtg ccgagccggg aataatgtag
     6961 ggcgcggccg ccgcggcaag cgcccggccc agaaatccgc gccgcgtcag cttctttcca
     7021 cccgtcttgc cttgcttcat tttgcctgcc tcatccatcg gttcttctca aaccatgttg
     7081 tgacttcgcg aggtttgcgg ccccgcccgc cgcgccggct ctttcgtgcc ccgctcccca
     7141 ccctgctatt cctcctggtg gtgcagcagc cagttggtcc actggtggtg atccgggttc
     7201 tttgatcgct ccagcaccag gttcagcagc cggtcgacct cctcccgatg ttccttgcgc
     7261 aacggtcccg cgacgccgat caccgccgcg ctcgcctcct ccaccacgtc ggggtcgtcc
     7321 atgtaatccg acagcactgc cagcgtcggc tcgatcgcca gctcgcccag cgccgaaatc
     7381 acccggcgct tctcctccgc attgacggcc agctcgatcg ccggccgcaa ctgctccagt
     7441 ttctcttccc gcgtcgcgtc gctcccgcgc accagttgga tgtaactgcg cagcgcaagg
     7501 atgcgccaga ccggcttttc cccttcgcgc gcgatccgca gcaggtccgg cgcggcctcc
     7561 accgtcggcc actcggtcag cgtccgcacc gctccgtcct tgagggccgg gtcggactcc
     7621 ccgaccgccg cccggaccgc ctccagcgcc tcccccgtgc cgagcagcgc caggctgtgc
     7681 agcagcgcaa cccgggccgc gaccggcgcc tcgctcaggc tcgacagcag cgtcggcagc
     7741 gccgcttccc gctcgcggcg cacgatcgcc tggagcgctt cccggaccgg tttcggcgct
     7801 tcgttcccct ccgcgcgctg caggtgctcg atcaccaccg gcaactgctc cagcgtcccc
     7861 atcgtcgcca gcgcctccca tcccgcggcg cggactgcgt tgttctcggc atccagatag
     7921 tccgtgattg tgcccaagct gtcgacttcc cggcgggcgg ccagcgcggc gatgatctcc
     7981 gcctgtgctc ccgggtccgc cgcgggcagc atcgcgacca actgcgcgcc cgtcgcttcg
     8041 gccggcagta actccaggct cgtgcgcgcg gcgtcgcgtt cagcttccac ctccgactgc
     8101 gccgcccgga tcagctcggc cagatccgtc tcgtcgccga caaaccccag cgcgcggatc
     8161 gccgccagcc gcaactccgc cgcgtcgctc cgcacggccc cgcgcgcgaa gtcccgcgct
     8221 tccgttccca actggctgcg gagcgtgtcg agcagaatca cctggctcgc gcgcggcaag
     8281 tcgctcagca tcgcgctgaa gtggcgcacg gctgccgggt cgtccgcatc ggcgatcgtc
     8341 tccgccgcca cgcgcatcag ccgcgcgtct tcgcccgcga gcgcctccgc caggtgctcg
     8401 cgcgcgtgct ccggctccgc gtccaccagc ccgcgcaggc aggccgcccg ccaccgctcg
     8461 cccccctcca gggccagcac ctcgcggtac agcgcggcgg cttcttcgtt cagcccggcc
     8521 cgctgcatgc gccgcgccgc gatcagccgg gcatcggtga tcgcgtcgcg cagctcggcc
     8581 ggggcctcgg cctgcaactc gcccagcgcg cgcgcgcttt cggccgtccc gatcatgccc
     8641 agcgcccgga tcgccgtcag caccgtctcg gaggacgccg catcgtcacg cgcccattgg
     8701 gtcagcggcg ccgtggcccc cgcgacgccg cgattgccga tcgaaccgat cgtcccgcgc
     8761 aacaggtcgc catcggttgt ctccagcgcc ccaatcagcg ctgcgtccac cacggggtcc
     8821 ggaatccgtt ccagtgccag ccgcgccgga tgcgcaagct cgcggacacc cagcagcgac
     8881 gcgagcgccg ccaccgaccg gccgctcccg atcagactga gccgttcgca ggcgaacttc
     8941 ttcgccgcct gcggcgcgtc gccccccagc accgccatca gccgcgtctc cagctcggcg
     9001 cgcgcggcct catccccccg cgattgcgcg atcgccgcat cgatcaccgt cagcggttca
     9061 cgcgtcgcct cccaggaata accggccagc gccgcgaatg ccgcatcgag ctcttccgct
     9121 cccgccggcg gatcggcggt tgctccggcg cccagcatcg cggtccacgc cgccatggcg
     9181 aacagcagcg cccgagccca tccgcccgcc ggcatcgttc ctctcacctt gcaacccatc
     9241 atccaaacct cactcttaaa atcgtccctc gtctctcgtc cctcgtcttc gtaatcgccc
     9301 ccaaaggctc acccaaaact cggctcttct ctctcgcccc gctctcgaaa cccctgctcc
     9361 ccggcgaggc acccccctgc gccccgccga ccgccctgcc gtgcaattcg tgtttttcgt
     9421 gatcccgtat cccgtacaaa aattccccat ccgacaacga tccgccgccg tgtgcgatcc
     9481 tccaattcct gttttgaact ttcaagcggt tcgtgggtca cggatccagc cgacgttcca
     9541 ggcaattcct gataaaaaaa tcagcatcac cacccgctcg ttgcccctgc gcccccttgt
     9601 ctcccgaccc gcgcttccgg ataatggacc agcgcccgcc atcgcgcgcc ccatcccgca
     9661 cagtcgttcg cttcatgaac ccacccgatc cgccggccaa cccgcctcac ccctccccgg
     9721 aggccccccg ccccacccgg ccggccccct cccgcggcca tcgtttcggc tggcgcgccc
     9781 ggaccgccct gctcctcggc gcgatcgctc tgcccgtcct cctcgtcgaa ttcggtctgc
     9841 gcgtcgtggg ctactcccac ccgctctacc ccgaaaaagt tcagttcggc tcccccaccc
     9901 gcgccgaaat tgacctctac ggcggctggg acgaggaccc gacccgcttc tggcttccca
     9961 ccgattaccg cgcaagactc cagcaggtcg gccgacccga cgtcgccttt ctcggctgct
    10021 cctgcacgat gtatggcgat tacgacgtcc acctggcccg catggtggcc gaccgccatc
    10081 ccggccgcgg cttctcctac atcaacctgg gcgtcctcgg ctggaccagc caccagggct
    10141 tgcagcagtt caaacaggac gtcatccccc tccagcccaa agtcgccacc gtctacttcg
    10201 gctggaacga ccattggatc ggcttcggcg tcgaggacaa ggaggtcgcc cgcctgaacg
    10261 aatccctcct cttcaagctg cgcaacgtcc ggctgcttca gctcgcgcgc gacgccttca
    10321 tcaaactgcg caaggatccc gacgccgggt acccgttgcg cgtctcactg gcggattatc
    10381 gcgctaacct gcgcgagatg gcgcgtctgg cccgcgagca cgacatccag ctcgtgctca
    10441 tgaccgcgcc cgatgggttc gtgcccggcg aggagcagcg ttacgcccat ctggtccccg
    10501 gcttcgtcgc ttcgacggag aagctgcgca acctgatccc gctgcacgag gcgtatgtcg
    10561 aagccgtgcg cgacgtcgcg cgcgaggagc ccggcgtgat cctctgcgac ctcgccgccg
    10621 agttcgacca gcgcttctct caagaggaaa tttccgagcg cctgttcacg cccgacgcga
    10681 ttcacctcaa gagcgtcggc tgccaggcga tcgccgagat cctgctcgaa tgtctggaac
    10741 agcacggcct gctggaggaa atcctgcgcc cggccgcgga ggtcactcag ctccccggcc
    10801 gcggcgtgcc ataacgatca ggatcgggat cgcgatcgac tcctgcccgc ccgcatccca
    10861 caaaaacgcc ggccgcgcca cgagccaccc tgcggcctcg tcagcgcgaa tctcttattt
    10921 cttgtttgaa aaaggagtta acgaaacgtc gacgggaagg cctactcggc ggcggcgccg
    10981 ccctcacaca gccccagcac ccggttgcgt tcggtgtccg acagatgatc gaacgtgctg
    11041 agcgctttct tcatcttgtg catgctcagc gagcgcagcg cgtcgttcat cagcaggtaa
    11101 agcggatcct ccggtcccat cgacagcacc acg
//
LOCUS       DAOPCO010000121        11117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5749_length_11117_cov_4.924697, whole genome shotgun sequence.
ACCESSION   DAOPCO010000121 DAOPCO010000000
VERSION     DAOPCO010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5749_length_11117_cov_4.924697"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="PLS90_09660"
     CDS             <1..1196
                     /locus_tag="PLS90_09660"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HPK02708.1"
                     /translation="TGVEALYADLGHFGRRPIRLAWGCIALPALLLNYFGQGAHLLAH
                     PEAADHIFYALVPTWGRVPMVVLATVATIIASQAVITGAFSLTRQAIQLGLFPRFRVI
                     HTSARRIGQIYVPPVNWALMICTLALVVAFGQSSKLAAAYGVAVNLTMLVSSILFFVV
                     ARCVWRWRWWAALPAAAVFITVDLAFFTANLSKVMHGAWFPLVVAAVVFSLMATWRRG
                     RELLGAQIRERLVSWDAFRARLAADPPPRVAGSAIFLTGNPDTVPVALLHNLEHNKVL
                     HSRIALLHFVPRETPYVEEKDRVRVVAMGENMTRIIADYGYMEEPSVRQTLARARELG
                     VDFDPTATSFFLGREKLSHGPGSPFGPVRHLLFGFLTLFSTDAATYFEIPLSQVIEVG
                     ISLEL"
     gene            complement(1561..2883)
                     /locus_tag="PLS90_09665"
     CDS             complement(1561..2883)
                     /locus_tag="PLS90_09665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HPK02709.1"
                     /translation="MYSRPLIRSVLVLFSVLLVTTSSRLPADPVLDWNQIACEAIRAD
                     PHHAAPTYSTRDFAMVHVAILDAFVAIRPFCRPYAAHVDAEPGASVDAAMAQAAWTVL
                     VALFPAQAAELNAALADALAAIPAGPARDAGVTVGNAVAQQILDLRSQDGAENLVDYE
                     PGTDPGHWRPTFPNFAPALGPHWGNVTPFVLESAPEFRPPPPPPLDSPQYAADFELLK
                     SIGVESASSRTDEQTEIGIFWAYDRFGMGPPTILYNRFLVDLVLELGNTVEQNARLFA
                     LANLAMADAGIVTWEAKYYYNFWRPITAIREGANDGNSATDGDPDWVPLGAPGGDGPS
                     FTPPFPSYPSGHAAFGAALYQALANFYVTDAMSFSATSEEMPGVIRNYDSFRAAADEN
                     GISRIYLGVHWTFDNTEAANLGRNVADYVFAHAGLEENAVDRSWTHYH"
     gene            complement(3471..4268)
                     /locus_tag="PLS90_09670"
     CDS             complement(3471..4268)
                     /locus_tag="PLS90_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPK02710.1"
                     /translation="MTSLDLSDPNLPAALDLAVAAAREAGDILRRLGGQPVQVLSAEG
                     RDIKTQADRDAEAAILARLRGASPWPILAEESGDQGVPDVGPFWVVDPLDGTLNFARG
                     IPLATVAIALMEADRPLLGVIYDFYRDELFRGLAQPGAGLWEIAGGQHPLSVSAVSDP
                     RQAILATGFPTNRDYSEPALREMIGQIQRFKKVRMLGSAAWMLAAVACGRVDAYAEDD
                     LYLWDVAAGVALVQAAGGYVELNPSPRGRWARRVRVASRKSLWRPPA"
     gene            complement(4492..5424)
                     /locus_tag="PLS90_09675"
     CDS             complement(4492..5424)
                     /locus_tag="PLS90_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02711.1"
                     /translation="MTLRIAIGPSTFGAEDDTPRRMLEEAGLEVVPNPHGRRLTEHEI
                     IHHLHDCDGLVAGLEPLNRRVLASTGHRLKAIARVGIGTTNVDLDAARELGIKVSNTP
                     EPPARAVAELTLGAMICLCRRLVPINAAMHTGEWPKEVTLGLLGARVLLVGYGRIGRR
                     VGELLRVLGAEVLVCDPELNPDSLLHGERLVTLADGLAEAEIISLHASGDRCLLGSAE
                     FDRMRRGVFLLNSARGELVDEEALIDALEAGWLGGVWFDAFWNEPYAGRLLRFPQVLL
                     TPHVATYTRQCRLAMEREAVENLLADLGITRVRP"
     gene            complement(5421..6509)
                     /locus_tag="PLS90_09680"
     CDS             complement(5421..6509)
                     /locus_tag="PLS90_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02712.1"
                     /translation="MLHIGIIGLGKMGRIRAAEIARHPRTQLVLAADPDPAAADTLPG
                     LTVTRDWRELIDAELDAVVVCAPNDITAAAVIAALRAGRHVLSEKPPGRNLAEVEAIR
                     RAAAARPDLKLKFGFNHRYHAGIREAKRIIDAGRLGRILWLRGIYGKCGGPGFEHSWR
                     SDRQRAGGGILLDQGIHMLDLFRYFCGDFVEVKSMVATAHWPISVEDNAFALLRDPHG
                     RIAMLHSSSTQWKNRFHLEIFLSEGYLSINGLLTSSRSYGDESITVARKQFDDGFATG
                     KPREEIIYFDTDPSWELELAEFVDCIENDQPVRHGTIDDALAVMRMVYAIYEDDGAGA
                     GPCRVGASPLADQSPPEPAHTPPDRATR"
     gene            complement(6521..7141)
                     /locus_tag="PLS90_09685"
     CDS             complement(6521..7141)
                     /locus_tag="PLS90_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK02713.1"
                     /translation="MHPLAQMFTQSASPADYARAYFRRLAALMDAVDPDAIQRAVEAF
                     ETVCREGRSIYAMANGGSAAVASHLVNDAVAGAFCDAHPPLRAWCLSDNIETVTALAN
                     DCGFDQVYERQLMGLMAPGDLVLALSVSGNSPNLVRAMQYARDHGARTMAWTGMAGGR
                     LGELAEIHLHLPCGADEYGPVEDMFSVLEHVLMTWLTQKRGRWLHH"
     gene            complement(7141..8154)
                     /locus_tag="PLS90_09690"
     CDS             complement(7141..8154)
                     /locus_tag="PLS90_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK02714.1"
                     /translation="MKHVVVTNDLREADIRPPEILSEFMRLSIAEAARFFDDPADLIQ
                     SDCPACASEAHEPVFTKHGFEYEQCLRCQSVFVARRPAPERLEDYYRNSRAVRYRAET
                     FTQATGPARRLHIQRAHANWLGRLYDECGGAGPGAYADVGTHYPMLFEEVRGLGLFAA
                     CHTINPLPGLEDACRQAGAATGALPAPLAAISSFEQLEHQFSPLEYLMDLNRLLAPGG
                     MIFLTTRTISGFDLQVLWDQAPYIYVPEHLNLLSVEGLELLFGLAGLEVVELSTPGQL
                     DLELIREAAARDPRVRLPAFVRYLLERREPRTHEDFQHFLQRNRLSSHVRIAAVKSPA
                     GEA"
     gene            8401..9648
                     /locus_tag="PLS90_09695"
     CDS             8401..9648
                     /locus_tag="PLS90_09695"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPK02715.1"
                     /translation="MKIVLAYSGGLDTSVILKWLRETYEAEVIAFCADLGQEEELADV
                     PEKARNTGASKCYVVDLVEEFAADFIYPMIQAGAIYEGQYFLGTSIARPLIAKAQVEI
                     ARREGADAVAHGATGKGNDQCRFELTYAALAPELKIISPWREWDFKGRTDLINYAKAH
                     GIPVAATAAKPYSTDRNLLHISFEAGVLEDPWAEPPAECYVLGTPIEQAPDKPDIIEL
                     EFEAGNAVALKGPDDEAPKRLSPAAMLRALNKLGGRHGVGRVDMVENRFVGMKSRGVY
                     ETPGGAILHQAHRQIESLTLDRETMHLRDALIPRYAEMVYYGFWYAPEREALQALVTE
                     SQRHVTGTVRLKLYKGNIITLGRKSPVSLYQTQVASMEDDAGAYNQADAGGFIRLNSI
                     RLRARTNQQTLPHVARQSEMKLG"
     gene            9810..9884
                     /locus_tag="PLS90_09700"
     tRNA            9810..9884
                     /locus_tag="PLS90_09700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9843..9845,aa:Gly,seq:tcc)
BASE COUNT         1836 a         3116 c         4338 g         1827 t
ORIGIN      
        1 tgacgggggt ggaggcgctc tatgccgatc tggggcattt cggccggcgg ccgatccggc
       61 tggcgtgggg ttgcattgcg ctgccggcgc tgctgctcaa ctacttcggc cagggggcgc
      121 acctgctggc gcatccggag gccgccgacc atattttcta cgcgctggtg ccgacctggg
      181 gccgcgttcc gatggtcgtg ctggcgacgg tggcgacgat catcgcgtcg caggcggtca
      241 ttacgggggc gttttcgctg acgcgccagg cgatccagct cgggctgttt ccacggttcc
      301 gcgtgattca cacgtcggcg cggcggatcg gccagattta cgttccgccg gtcaactggg
      361 cgctgatgat ctgcacgctg gcgctggtcg tggcgttcgg gcagtcgagc aagctggccg
      421 cggcgtacgg cgtcgcggtc aatttgacga tgcttgtctc gtcgatcctg tttttcgtgg
      481 tcgcgcgttg tgtctggcgc tggcggtggt gggccgcgct gccggcggcg gcggtgttca
      541 tcaccgtcga tctggcgttt ttcacggcga acctgagcaa ggtgatgcac ggcgcctggt
      601 ttccgctggt cgttgcggcg gtcgtcttca gcctgatggc gacctggcgg cgggggcgcg
      661 agctgctcgg ggcgcagatc cgggagcgtc tggtgtcgtg ggatgcgttt cgggcgcggc
      721 tggcggccga cccgccgccg cgcgtcgcgg gcagcgcgat cttcctgacg ggcaatcccg
      781 acaccgtccc cgtagcgctg ctgcacaacc tggaacacaa caaggtgctt cactcgcgga
      841 ttgcgctgct ccacttcgtg ccgcgcgaga cgccgtacgt ggaggagaag gatcgcgtgc
      901 gcgtggtcgc gatgggcgag aatatgacgc ggatcatcgc cgactacggg tatatggagg
      961 agccgagcgt caggcagacg ctcgcccggg cccgcgaact gggggtcgat tttgatccca
     1021 cggcgaccag cttttttctg gggcgggaga agctgtcgca cgggccgggc tcgccgttcg
     1081 gcccggtgcg gcatctgttg ttcgggttcc tgacgttgtt ttcgaccgat gcggcgacgt
     1141 attttgaaat tccgctcagc caggtgattg aagtcggcat ctcgctggag ctatagcgga
     1201 cggcggggag ggcggctgag aagccggcga tccggccacc ccttgcagcg gtttgggagt
     1261 tgggggcggg gacgcagggt tccggcctgc ggccgtcacc ctgcgcttta atccggtcgt
     1321 ccgctgcgcg gactggggca ttcgccccct tcggggacgg ggggtggggt gtcgtggccc
     1381 caggttgcgc tgcgctgcac ctggggctat ccaaattgca cctcttcgag gcggcctgtg
     1441 gcatccctgc cgggatgcgg tgaaatgggg tggccggatg accggtggtg tcgctgcgct
     1501 cgaccaccgg ctacttgctg gcatccctgc cgggatgcgg gacaggactc tcacaccggc
     1561 tcagtggtag tgggtccagg agcgatcgac ggcgttctcc tcgaggccgg cgtgggcgaa
     1621 gacgtaatcg gcgacattgc ggcccaggtt ggcggcctcg gtgttgtcga acgtccagtg
     1681 gacgccgagg tagatgcggc tgatgccgtt ttcatcggcg gcggcgcgga acgaatcgta
     1741 attgcggatg acgccgggca tctcctcgga ggtcgccgag aagctcatcg cgtcggttac
     1801 gtagaagttg gcgagggcct gatagagcgc ggcgccgaag gcggcatggc ccgaggggta
     1861 agacggaaag gggggcgtga agctgggccc gtcgccgccg ggggcgccca gggggaccca
     1921 gtcgggatcg ccgtcggtgg cggagttgcc gtcgttggcc ccctcgcgga tggcggtgat
     1981 cgggcgccag aaattgtaat agtacttggc ttcccaggtg acgatgcccg catcggccat
     2041 ggcgaggttg gccagggcaa agaggcgggc gttctgctcg acggtgttgc ccagctccag
     2101 gacgaggtcg acgaggaagc ggttatagag gatcgtggga ggccccatgc cgaagcggtc
     2161 gtaggcccag aagatgccga tctcggtctg ctcgtcggtg cgggagctgg cggattcgac
     2221 gccgatggat ttgagcagtt caaaatcggc ggcatattgc gggctatcga gggggggcgg
     2281 gggcggcggg cggaattcgg gggcgctttc gaggacgaac ggggtgacat tgccccagtg
     2341 gggcccgagg gccggggcga agttggggaa agtcggccgc cagtggcccg ggtcagtccc
     2401 cggttcgtag tcgacgaggt tttcagcgcc atcctggctg cggagatcga ggatctgctg
     2461 ggcgacggcg ttgccgacgg tcacgccggc gtcgcgcgcc gggccggcgg ggatggccgc
     2521 cagggcgtcg gccagggcgg cgttgagctc ggcggcctgg gcggggaaga gggcgacgag
     2581 gacggtccag gcggcctggg ccatggcggc gtcaacggaa gcgcccggtt cggcgtcgac
     2641 gtgagcggca tagggccggc agaaggggcg gatggccacg aaggcatcga ggatcgcgac
     2701 atggaccatg gcgaaatcgc gggtcgagta agtcggcgcg gcatggtgcg ggtcggcgcg
     2761 gatcgcttcg caggcgatct ggttccagtc cagaaccgga tcggccggga gccgggagga
     2821 ggtcgtaacg agcaggacgg agaacaggac gagaacagac cgaatgagag gccgggaata
     2881 caagcgggga tacctcgctg gacggatgga tcgaaggtga ccgaggggga atggagaaac
     2941 tcccctcggg atcaagttgg cggtctttgt ccgggaccgt catgggcggc gtttcggtga
     3001 gcggcgggaa ggggcaccgt gaggggatga cgcacgccga tgaaggcgga gaggtccggg
     3061 gggctgaagg ggtatcgccg cggcgggcgc gggctgagcc gcgcgccgcg atgagccgat
     3121 ccatcggcgc ggcggaggcc gcgcgccggc gccgtgaggg gcggcggaac gccgcgcatg
     3181 tcctcacggg aaagcgaaag ttccgggagg aatttacgcc ggagggataa atgaggtcaa
     3241 gatcatcttt ttggcggggg cggggggtgg tggggggatg ggggatccgg tggacggggg
     3301 ggagtttttt cgagagccga ggacgaggga cgagagccga gagccgaatt ttgggcgggc
     3361 cgttgggggc gattacgagg acgagggacg agagccgaga gccgaatttt gggcgagtcg
     3421 ttgggggcga ttacgaggac gagggacgaa gacgagggac gagagccgaa tcaggcgggg
     3481 gggcgccaga gggacttgcg gctggcgacg cggacacggc gcgcccagcg gccgcgcggc
     3541 gaggggttga gttcgacata gccgccggcg gcctgcacca gcgcgacgcc cgccgccacg
     3601 tcccacaggt agagatcgtc ctcggcgtag gcgtcgacgc ggccgcaggc gacggcggcg
     3661 agcatccagg cggccgagcc gagcatccgc accttcttga agcgctggat ctggccgatc
     3721 atttcgcgga gggcaggttc gctgtaatcg cgattggtgg ggaagccggt ggcgaggatc
     3781 gcctggcgcg ggtcggagac agccgagacc gaaagcgggt gctggccgcc ggcgatctcc
     3841 cagaggccgg cgcccggctg ggcgaggccg cggaagagct catcgcgata gaagtcgtaa
     3901 atcacgccga gcagggggcg atcggcctcc atcagggcga tggcgacggt ggccagcggg
     3961 atgccgcgcg cgaagttgag cgtgccgtcg agcggatcga cgacccagaa tggtccgacg
     4021 tcggggacgc cctggtcgcc gctctcctcg gcgaggatcg gccaggggct ggcgccgcgc
     4081 aggcgggcga ggatggcggc ttcggcgtcg cggtcggcct gggtcttgat gtcgcggccc
     4141 tcggcgctga ggacctggac cggctggccg cccagtcggc ggaggatgtc gcccgcctcg
     4201 cgcgcggcgg cgacggcgag gtcgagggcg gccgggaggt tggggtcaga gagatcgagg
     4261 gaggtcatgg ggaactccgc gttggggtga ccgataatcc ggggcgcatc ctggcatgac
     4321 tcgaatgcag gtcagttttt cagcatcagc ccaggtcagc ggatccaaac cggtgtcgac
     4381 gcgttgcggt gcaacgctct gccaccgcac tccacgagag ttgcctcgac gtcgctccct
     4441 gctcgttgac cagcgagggc gccggtctgc ggcggcgcgg ttgcggcgtc atcaaggccg
     4501 gaccctggtg attcccaggt cggcgaggag gttttcgacc gcctcgcgtt ccatggcgag
     4561 ccggcattgg cgggtgtagg tcgccacgtg cggcgtgagc aggacctggg ggaagcgcag
     4621 gaggcggccg gcgtagggct cgttccagaa ggcgtcgaac cagacgccgc cgagccagcc
     4681 ggcctcgagg gcgtcgatca gggcctcctc gtcgacgagt tcgccgcggg cggagttgag
     4741 caggaagact ccgcggcgca tgcggtcgaa ttcggcggag ccgagcaggc agcgatcgcc
     4801 gctggcgtgg agcgagatga tctcggcctc ggccaggccg tcggccagcg tgacgaggcg
     4861 ctcgccgtgc aagagggaat ccggattgag ttcgggatcg cagacgagga cctcggcccc
     4921 caggacgcgc aacagttcgc cgacgcgccg gccgatgcgg ccgtagccga cgagcagcac
     4981 cctggcgccg agcaggccga gtgtcacctc cttgggccat tcgcccgtgt gcatggcggc
     5041 gttgatcgga acgagccggc ggcagaggca gatcatcgcg ccgagggtca gctcggcgac
     5101 ggcgcgcgcg gggggttcgg gggtattgga gaccttgatg ccgagttcgc gggcggcgtc
     5161 cagatcgacg ttggtcgtgc cgatgccgac gcgggcgatg gccttgagcc ggtggccggt
     5221 ggaggcgagg acgcggcggt tgagcggctc gagaccggcc acgagcccgt cgcagtcgtg
     5281 caggtggtgg atgatctcgt gttcggtgag tcggcggccg tgggggttgg ggaccacttc
     5341 gaggccggct tcctcgagca tgcggcgggg ggtgtcgtcc tcggcgccga aggtagacgg
     5401 accgatggca atgcgcagcg tcatcgtgtc gctcgatcgg ggggagtgtg cgccggttcc
     5461 ggcggcgact ggtcggccag ggggctggcc ccgacgcgac acggtccggc gccggcgccg
     5521 tcatcctcgt agattgcata aaccatgcgc atcacggcca gggcgtcgtc gatggtgccg
     5581 tggcggacgg gctgatcgtt ttcgatgcag tcgacgaatt cggccagctc aagttcccag
     5641 gaggggtcgg tgtcgaagta gatgatttcc tcgcggggtt tgccggtggc gaagccgtcg
     5701 tcgaactgct tgcgggcgac ggtgatcgac tcgtcgccgt agctgcggct ggaggtgagc
     5761 agcccgttga tgctgaggta gccttcggag aggaaaattt cgaggtggaa gcggttcttc
     5821 cactgggtgg aggagctgtg gagcatggcg atgcggccgt ggggatcgcg cagcagggca
     5881 aaggcgttgt cctcgacgga gatcggccag tgggcggtgg cgaccattga tttgacctcg
     5941 acgaaatcgc cgcagaaata gcggaagagg tcgagcatgt ggattccctg gtcgaggagg
     6001 atgccgccgc cggcgcgctg gcggtcgctg cgccaggagt gttcgaagcc gggtccgccg
     6061 catttgccgt aaatgccgcg cagccagagg atgcggccga ggcggccggc gtcgatgatg
     6121 cgtttggcct cgcggatgcc ggcgtgatag cgatggttga agccgaactt gagtttgagg
     6181 tcggggcggg cggcggcggc gcggcggatg gcctcgacct cggccaggtt gcggccgggg
     6241 ggtttttcgc tgaggacatg gcggccggcg cgcagggcgg cgatgacggc cgcggcggtg
     6301 atgtcgttgg gggcgcagac gacgacggcg tcgagctcgg cgtcgatcag ctcgcgccag
     6361 tcgcgggtga cggtgaggcc gggcagggtg tcggcggcgg cgggatcggg atcggcggcg
     6421 aggaccagct gggtgcgcgg gtggcgcgcg atttcggcgg cgcggatgcg ccccatcttg
     6481 cccaggccga tgatgccgat gtggagcatg ggacgatcca tcaatggtgc aaccagcggc
     6541 cgcgcttctg ggtcagccag gtcatcagca catgttccag gacggagaac atatcctcga
     6601 cggggccgta ttcgtcggcg ccgcagggga gatggaggtg gatctcggcg agttcgccga
     6661 ggcggccgcc cgccatgccg gtccaggcca tcgtgcgcgc gccgtggtcg cgggcgtact
     6721 gcatcgcgcg gacgaggttg gggctgttgc cgctgaccga cagggcgagg acgagatcgc
     6781 cgggggccat cagccccatg agctggcgct cgtagacctg atcgaacccg caatcattgg
     6841 ccagggcggt gacggtctcg atattgtcgc tgaggcacca ggcgcgcagg ggggggtgcg
     6901 cgtcgcagaa ggcgccggcg acggcatcgt tgacgagatg ggaggcgacg gccgcgctgc
     6961 cgccattggc catggcgtag atcgagcgcc cctcgcggca gacagtttcg aaggcttcga
     7021 cggcgcgctg gatggcgtcg gggtcgacgg cgtccatgag cgccgccagc cgccggaaat
     7081 aggcgcgggc gtaatcggcc ggcgaagcgg actgggtgaa catttgggcg agcggatgca
     7141 tcaggcctct ccggcgggcg atttgacggc cgcgatgcgg acgtgcgaac tgagccgatt
     7201 gcgctggagg aaatgctgga aatcctcgtg ggtgcgcggt tcgcgccgtt cgagcagata
     7261 gcgcacgaac gccggcaggc ggacacgggg atcgcgggcg gcggcctcgc ggatcagctc
     7321 caggtcgagc tggcccggcg tgctgagttc gacaacctcc agaccggcga ggccgaaaag
     7381 cagttcgagg ccctcgaccg agaggaggtt caggtgctcg ggaacgtaga tgtagggcgc
     7441 ctgatcccag agaacctgca ggtcgaagcc gctgatggtg cgggtggtga ggaagatcat
     7501 gccgccgggg gcgagcagcc ggttcaggtc catcaggtat tcgagcgggg agaactgatg
     7561 ctcgagctgc tcgaaggagc tgatcgcggc gaggggggcg ggcagggcgc cggtggcggc
     7621 gccggcctgg cggcaggcgt cctcgaggcc gggcaagggg ttgatcgtgt ggcaggcggc
     7681 gaagagcccg aggccgcgga cttcctcgaa gagcatcggg tagtgcgtgc cgacgtcggc
     7741 gtaggcgccc gggccggcgc cgccgcattc gtcatacagc cggccgagcc agttggcgtg
     7801 ggcgcgctgg atgtggaggc ggcgggcggg accggtggcc tgggtgaaag tctcggcgcg
     7861 gtacctgacg gcgcgggaat tgcggtagta gtcctcgagc cgctcgggag cgggtcggcg
     7921 ggcgacgaac acgctctggc aacgcaggca ctgctcgtat tcgaagccgt gtttggtgaa
     7981 gacgggctcg tgggcctcgc tggcgcaggc ggggcagtcc gactggatca ggtcggcggg
     8041 atcgtcgaag aagcgggcgg cttcggcgat cgagaggcgc atgaactcag agaggatttc
     8101 cgggggccgg atatccgctt cgcgcagatc gttggtgaca accacatgct tcacgggggg
     8161 tctcgcgcgc gccgctcacg cggctgctgg gatgccgcag tcctgctctg gccggccaca
     8221 gtcaggaaca ggctagtgat tcgtgagcga ttcgtcaact tgcgagggcc gattcgcggg
     8281 tccggtcgcg gcgcactcgc cattgcaagg ggcggggcgg gtgcggcatg ataggcggtt
     8341 tgacgaggcg gggcgcggcc gaggcaaggc gcccggccac acacgcggag gaagggcgcc
     8401 atgaaaatcg tgctggctta ctcgggtgga ctcgatacct cggtcatcct gaaatggttg
     8461 cgcgagacgt atgaggcgga ggtgatcgcc ttctgcgccg acctggggca ggaggaggaa
     8521 ctggccgacg tgccggagaa ggcgcgcaac accggggcgt cgaagtgcta cgtggtcgat
     8581 ctggtggagg agttcgcggc cgatttcatc tacccgatga tccaggccgg cgcgatctac
     8641 gaggggcagt attttctggg gacgtcgatc gcgcggccgc tgatcgccaa ggcgcaagtg
     8701 gagatcgcgc ggcgcgaggg ggccgatgcg gtggcccacg gcgccaccgg caagggcaac
     8761 gatcagtgcc ggttcgaact gacgtatgcc gcgctggcgc ccgagctcaa gatcatttcg
     8821 ccctggcgcg agtgggactt caaggggcgc accgacctga tcaactacgc gaaagcgcac
     8881 gggattccgg tcgcggcgac ggcggccaag ccctactcga ccgaccgcaa cctgctgcac
     8941 atctcgttcg aggcgggggt gctggaggac ccctgggccg agccgccggc ggagtgctac
     9001 gtgctgggga cgccgatcga gcaggcgccg gacaagcccg acatcatcga gctggaattc
     9061 gaggcgggca acgcggtggc gctgaagggg ccggacgacg aggcgccgaa gcggctgagt
     9121 ccggcggcga tgctgcgcgc gctgaacaag ctggggggcc ggcacggcgt ggggcgcgtc
     9181 gacatggtag agaaccgctt cgtcgggatg aagtcgcgcg gggtatatga aacccccggc
     9241 ggggcgatcc tgcaccaggc gcatcggcag atcgaatcgc tgacgctgga ccgcgagacg
     9301 atgcacctgc gcgacgcgct gatcccgcgc tatgccgaga tggtgtatta cggcttctgg
     9361 tatgcgcccg aacgcgaggc gctgcaggca ctggtgaccg agagccagcg ccacgtgacg
     9421 gggacggtgc ggctgaaact ctacaagggg aacatcatca cgctggggcg caagtcgccg
     9481 gtgtcgctct accaaacgca ggtggcctcg atggaggacg acgcgggggc ctacaaccag
     9541 gccgacgcgg gggggttcat ccggctgaac tcgatccggc tgcgagcgcg gacgaatcag
     9601 cagacgctgc cgcacgtcgc gcgccagagc gagatgaagc tgggttaggc ggagcgggcc
     9661 ggaagccggt tgggggtcgg tcgggcgatc cggcgatgtt cccgtgatat gccggctgca
     9721 aggcgattcg tcgtcgagat gttctggtcg tccgaagcct tgacccaagg cgcgacgttg
     9781 gtagaatgac aaccagcccg cgcaagcgag cgcgtgtagc acaatggtta gtgcttcggc
     9841 cttccaagcc gaagatacgg gttcgattcc cgtcacgcgc tccacctgac ttcttcatca
     9901 tccctgaaac tattcgactt actagaagat gcctcgatct cgtcagtgcg tcaaactggc
     9961 gcgagaaggg acgttttgca tcgactttgt cacaaatctg gcacaaatgg ggcgcattaa
    10021 aaacgtgcgt aagtcggctt gctgaaggcg ctcagcaagc ctcttggctt cgcctcggaa
    10081 gcgtgaaaag cgcatgaaga ggggcgggaa gcgtgttttg tgtgtgaaaa cgtcatattc
    10141 gcctatctag cctcgactgg aatccccggt caaataggcg gatctggatt tgttccggta
    10201 tcctgggtag atccaaaagg tccgactgga acacgtcacg cggcagtgtc tttgtcagct
    10261 ttcgattgtt tgtctgcgta ccacccggta cccccccttg ccacgccagc agattgcttg
    10321 gagtccgagg ctgtatcaca gagccgccgt ccgagctgtg cccaatcggc tttgagtagg
    10381 tctgcttgtc cctcgaacct agaattaaaa aatcgcgcgt atagcagcgg ctcggtgagg
    10441 tgtctggcgc tctgtccatc ccacgcgggt gggtggagcg gcgtgcgcat cagcggcgcc
    10501 atcaacacca ggcaacagaa aggttgctga aatgaatgaa ctcgcgttgc gggttacacg
    10561 atgcaagatc gttccgaacc gggacgagga cagcaaactg aaggcgggtg cgtcactcgt
    10621 gctcaacgac gctttggcga tccaagccaa ggtagtccaa gggaatcgcc gactcctggt
    10681 cgccatgccc gctctcaggc ggaaagacgg ggaattccac gacgtggctt tcccgatcgt
    10741 tcgagagttg cgcgagcaca tcgaacaggt agtgttggcg gaataccaac gagtcacatg
    10801 tgtgaaggtc tccggatcaa cgcacggacg gttctcaacg gggcgtcaag ccagcaaaac
    10861 gccggtcggt cggcagacaa gcgctgtggg aactgggcga tgaagcggaa gccaaaccgt
    10921 agaggaggat atgcgtggat gcgagccgag gaggcgacgg ctttgctgga tgtgctcaag
    10981 aatggagggt caagggaggt gagctatcgc ggcaaaccca gaacgttgac gttggagccg
    11041 caaccggagg cttgggcacc cgtgttcttc ttgctgcact gtggggctcg gttgtatgag
    11101 gcatacgagt taaagtg
//
LOCUS       DAOPCO010000122        11099 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5757_length_11099_cov_4.154020, whole genome shotgun sequence.
ACCESSION   DAOPCO010000122 DAOPCO010000000
VERSION     DAOPCO010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11099
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_11099_cov_4.154020"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLS90_09705"
     CDS             complement(<1..810)
                     /locus_tag="PLS90_09705"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HPK02716.1"
                     /translation="MRGPTRHAPLFLLLLLGSFLQALLLFRSGAVHSNDFKHLWAGAF
                     LLLHDASPYVPENLFRVARAQHWDAINPFVYLPTTGLLLRPLAALGFQKAVAIWFWFN
                     WALAWAVVAWTPRLLRLPRGDFARLLGAVFLVGGMPFMRQMTAGQMNVVMAALLVLAA
                     AALLRRRSVLLGALLALAFAWKIAPALLIALLIPLRRPRAAVWAAGLTCLLLGASILL
                     HDWPVHLDAARIVSQMGYGRSTWSEFGNDFHRDPANQSINSLLHHLLTHNRF"
     gene            971..2533
                     /locus_tag="PLS90_09710"
     CDS             971..2533
                     /locus_tag="PLS90_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HPK02717.1"
                     /translation="MLNLDRGFVKVWFGPEPANKAAGRGPAGASPVEPARARPRRFGL
                     RLNQMAVLSRKRLPVWRTLTLMLSDVIAITLGLCVTYWIRFHSGWIDVSKGFITDDYQ
                     AIFPWAVLIWFLSLRFENLYRRRMRVFDFNVVRRIITGSCLALLILIAWNFYNKMGEY
                     SRLASLIMMGAVVAMLIANRLVMNVLFQWLLLRRGVGQSRTVVLGCGPIAESICRSLL
                     SHPEWGMQPIGMVAGHSERRPEHVVPGIPVLGTLKNLEALLSEHRADEVILTEPELER
                     SEIPHLLVECERAMAEFRIVPETTELLLSGMTVETLDGIPLLGIRETPLQGWNAALKR
                     MVDFSAALVGLMLTAPLIGLLAWLIRRQDGMSPFYVQERMGNDGRLFNIIKLRTMRPD
                     AEEQSGPIFATDRDERCTPLGMLLRRTHLDELPQLINVLRGDMSLVGPRPERPFFVEQ
                     FRDNVPRYMARHKVKSGITGWAQIHGLCGRHGSISERLKYDLYYIENWSLWLDIKILA
                     RTVFGAIRPGPV"
     gene            complement(2894..3610)
                     /locus_tag="PLS90_09715"
     CDS             complement(2894..3610)
                     /locus_tag="PLS90_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPK02718.1"
                     /translation="MSDLGRQTNNPPADLRERLAAVRERIERAARRAGRDPAAVELVA
                     VTKTHPADAVRAAIDAGQSLFGENRVQEALPKMDAVGPGPRWHLIGHLQRNKAAAAVG
                     RFELIHSVDSTRLIEELERRAAAADTQQAILLQLNVAGEASKFGAAPADLPALLAALA
                     AAPHLRALGLMTIPPWDDDPERTRPHFSALRRLLEEAQGPPNFEKKHLSMGMSGDFEV
                     AIEEGATLVRLGSALFGSRL"
     gene            3655..4515
                     /locus_tag="PLS90_09720"
     CDS             3655..4515
                     /locus_tag="PLS90_09720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02719.1"
                     /translation="MDSPTAPRSKLTALLPTFNEEAHLAGCIDSVAWADEILVVDSFS
                     TDATREIARARGARVVEHEYVNSAAQKNWSIPQCSHEWVLVVDSDERVTPELRREIEG
                     VLTADANGRPVADGYRIGRINHFLGRRIEHCGWQNDTVLRLFRRDKGRYQEREVHADV
                     LVEGKIGRLRHKLLHYTFRDFEQYMRKFDRYTSWAAGDRGRRTPRVGYTHLLLRPLGR
                     FVKQYVLKRGFLDGRVGLVVCLLASYSVFMKYAKLLERQLQAEGGGAPGESEAAARAE
                     GAAASLDMKA"
     gene            4512..5678
                     /gene="waaF"
                     /locus_tag="PLS90_09725"
     CDS             4512..5678
                     /gene="waaF"
                     /locus_tag="PLS90_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPK02720.1"
                     /translation="MTVSVAPPPLPPSADRVDGAELPPGQAAPGSPLERLLRRQRAES
                     LRVFIKGVNWVGDAIIATPALAHLRRQFPDVQITLMVRPWVAAIYENSSDIDELWVHD
                     DSKDLNAFVKAVRMVRKGRFQIGIALPNSLRSGLLLRLASVPYRFGYHTSLARRMLLN
                     YGQTPASDLLAGHQVFYYLGIIEGMVGQAARRQQLRLTVGELEREEIRRLLAQRGLDR
                     GRPIVGLAPGSINSQAKRWPAERYAELADLLGRKTAAEIVLLGSGKEKEVCDRVATLC
                     HQPVHNLAGELNLGQVIALMERLQALICNDSGAMHIAAALRVPTLAIFGPTEWTTTYP
                     FSNAANIIRKEGVACAPCMLRECPIDHRCMTGIAVGDVLDGFRKVLQQAKQYGR"
     gene            5763..6932
                     /locus_tag="PLS90_09730"
     CDS             5763..6932
                     /locus_tag="PLS90_09730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02721.1"
                     /translation="MNSDEPPSTVNQPAPRVVHLDDGRLWRGGQHQVWLLLGELARRG
                     IAQMLVAHDGCPLTLRAREAGIEVETVHFRGEWDWRTPRRIAALADAFGANLLHAHTS
                     HAHTLGLRALRQMRQRGMLVTTRRVDFAIRRNILSRRKYKLPGQHFIAISRAVRDVLV
                     AGGVAPERIDVVHSGVPPIAAEKLEPRAAVRAELGLASDEIAIVNIGALTDHKGQRWL
                     IEAGPEVFRAFPRARIHILGEGELRPALEQRIRELNLRGRVILHGHVENARLKLAAFD
                     LFVSSSHLEGLGTVNLDAMLAGVPVVAAAAGGVPEIVEDGRTGRLVPARDAGALARAI
                     VEALSNPAETAAMIERGRRHVEEHFSAASMAAGTLAVYRKLLAAHAAAGFVALGR"
     gene            7316..8344
                     /locus_tag="PLS90_09735"
     CDS             7316..8344
                     /locus_tag="PLS90_09735"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HPK02722.1"
                     /translation="MSKEPILLFGLPRSGTTWLGKIFDSHPATFYRHEPDSLKRFGTV
                     PLIVDAGGAAAWRDQVGPYVEQIATMRSAKLWKYPLFPKHYCRGGRFALLRASVFAGK
                     SMVKVFGDFPVPNFVRADQRGDIRLVWKSIESVGRLGLIVRTLPAVRVILILRHPCGF
                     VASILRGEAAGKFGRGWALSHDLTIMEKLTACEQARRRGITVERMQAMEPIERLAWHW
                     ALSNEKAMEEIEGRDDCMVLRYEDVCAHPVEESRRLFAFAGLAWDAQTEEFLARSTAS
                     EQNDYYSVFKNPLVAANKWRNQLSVQQVEQVLSIARQSVPGRLYDSWKFDEASKTVPS
                     ARPLQVSR"
     gene            9117..10796
                     /locus_tag="PLS90_09740"
     CDS             9117..10796
                     /locus_tag="PLS90_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK02723.1"
                     /translation="MGDGNSSPVRERRAPALISGNEALALAARDAGIALGTGYPGTPS
                     TEILEELDRQGGRAQWAPNEKVALEVALGAAYCGARALVTMKHVGLNVAADPLFTAAY
                     TGVAGALVVVTADDPGMASSQNEQDNRHYAAAARLPMLEPADSQQAYEFLLTAVWISE
                     RWGIPVLLRPTTRVCHGKSIVRFERGAPAAPAARYEKDVRSRVMIPAHAGPAHRALEA
                     KLKAIAAWNDERGPNQAVSRGVSGAPAIIASGIAAMHALEAAPQADVLMLGMTYPPPL
                     GLIRRFAAAAAAEGHPLHVIEEGDPVLAERLRAAGLNVQSAPEDYRFGELNVNRVRAI
                     LSGETAPTAATPAGQPPRLCTACPHRSVFSVLRDLNCIVAGDIGCYTLGVLPPYEALD
                     TCVCMGASIGVGLGMRHVLPREEAKRVVSVIGDSTFIHSGLTGLVEMVYNPPPTGHVV
                     LILDNGTTAMTGLQEHPGTGRRLGHEPTARVDFEEVIRAIGIREVHVIDATEDVQAFE
                     TLLRVCLDGEQPAVIIARRPCMLAAGRIRRYEKAAAETAAGACGCAEGEER"
     gene            10796..>11099
                     /locus_tag="PLS90_09745"
     CDS             10796..>11099
                     /locus_tag="PLS90_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPK02724.1"
                     /translation="MGASGAGGGGGVVSVVIAGLGGQGVLKASDILSEAAFAAGLDVK
                     KSEVHGMSQRGGSVASDVRFGAKVYSPMAPVGEADYLVVLAEDQVEVFRHRLSPRGE"
BASE COUNT         1774 a         2930 c         4624 g         1771 t
ORIGIN      
        1 gaagcggttg tgcgtcagca ggtgatgcag cagcgagttg atcgactggt tggccgggtc
       61 gcgatggaag tcgttgccga attcgctcca ggtggagcgg ccgtagccca tctgggagac
      121 gatgcgggcg gcgtcgagat gaaccggcca gtcatggagc aggatcgacg cgccgagcag
      181 gaggcaggtc aagccggcgg cccagacggc ggcgcggggg cggcgcagcg ggatgagcag
      241 ggcgatgagg agcgcggggg cgatcttcca ggcgaaggcg agggcgagga gggcgccgag
      301 gaggacggag cgccgccgca gcaacgcggc ggcggcgagg accagcaggg cggccatcac
      361 gacgttcatc tgtccggccg tcatctggcg catgaagggc atgccgccga cgaggaagac
      421 cgcgccgagc aggcgcgcga agtcgccgcg cggcagccgg agcaagcggg gggtccaggc
      481 gacgacggcc catgccaggg cccagttgaa ccagaaccag atggcgaccg ccttctggaa
      541 gccgagggcg gccaatggac ggaggagcag gccggtggtg gggagataaa cgaacggatt
      601 gatggcgtcc cagtgctggg cgcgcgcgac gcggaacagg ttttcgggga cgtaggggct
      661 ggcgtcgtgg agaagcagga aagcgccggc ccagaggtgc ttgaaatcgt tggaatggac
      721 ggcgccggag cggaagagca gcagggcctg aaggaaggag ccgaggagca gaaggagaaa
      781 caggggggcg tggcgggtcg gaccgcgcat gggcgaggcg accgggggga ggctggctgg
      841 ggtgggggat tcggcttcca cggcggaggg tcatcccaag tcggagcggg attcggcgcc
      901 gcgggttgcg ccccgggggg cggacaaagg gagcagcggg gacggagggt caggcgcggg
      961 ccggtcggta atgttgaatt tggaccgggg ttttgtcaaa gtgtggttcg ggcccgaacc
     1021 cgccaacaag gccgccggcc gcggaccggc cggcgcatct cccgtcgaac cggcccgcgc
     1081 ccgcccgcgc cgtttcggcc tccggctgaa ccagatggct gtcctgagcc gaaaacgcct
     1141 ccccgtctgg cgcacgctga cgctgatgct gtccgacgtg attgcgatca cgctcgggct
     1201 gtgcgtcacg tactggatcc gatttcattc ggggtggatc gacgtcagca aggggttcat
     1261 cacggacgat taccaggcga tctttccgtg ggcggtgctg atctggtttc tgtcgctgcg
     1321 attcgagaac ctttaccggc ggcggatgcg cgtcttcgat ttcaatgtgg tgcgccggat
     1381 catcaccggc tcgtgcctgg cgctgctgat tctgatcgct tggaatttct acaacaagat
     1441 gggggagtat tcgcgcctgg cctcgctgat catgatgggg gcggtggtgg cgatgctgat
     1501 cgccaaccgg ctggtgatga acgtgctgtt ccagtggctg ctgctgcggc ggggggtggg
     1561 gcagtcgcgg acggtggtgc tggggtgcgg gccgatcgcg gaatcgatct gccggagcct
     1621 gctgagtcat ccggagtggg ggatgcagcc gatcgggatg gtggcggggc acagcgagcg
     1681 ccggccggag cacgtggtgc cggggattcc ggtgctgggg acgctgaaga acctggaggc
     1741 attgctgagc gagcaccgcg ccgacgaagt gattttgacg gaaccggaac tggagcgatc
     1801 ggagatcccg catctgctgg tggaatgcga gcgggcgatg gcggagttcc ggatcgtgcc
     1861 ggagacgacc gaactgctgc tgtcggggat gacggtggag acgctcgacg ggattccgct
     1921 gctggggatc cgcgagacgc cgctgcaggg gtggaacgcg gcgctgaagc ggatggttga
     1981 tttcagcgcg gcgctggtgg ggctgatgct gacggcgccg ctgatcgggc tgctggcgtg
     2041 gctgatccgg cggcaggacg ggatgtcgcc gttctacgtg caggagcgga tggggaatga
     2101 cggtcggctg ttcaatatca tcaagctgcg gacgatgcgg ccggatgcgg aggaacagag
     2161 cgggccgatc ttcgcgaccg accgggacga gcggtgcacg ccgctgggga tgctgctgcg
     2221 gcggacgcat ctggatgagc tgccgcagtt gatcaacgtg ctgcgcggcg acatgagcct
     2281 ggtgggcccg cggccggagc ggccgttttt cgtcgagcaa ttccgcgaca acgtgccgcg
     2341 ctacatggcg cggcacaaag tgaagagcgg gatcacgggg tgggcgcaga tccatgggtt
     2401 gtgcgggcgg cacgggtcga tcagcgagcg gttgaaatac gatctgtatt atatcgagaa
     2461 ctggtcgttg tggctggaca tcaagattct ggcgcggacg gttttcgggg cgatccggcc
     2521 ggggccggtg tgagggggga tgcggaaggg agtgggcggg gtggacgggg tttcgagagc
     2581 cgaggacgag ggacgagagc cgagagccga attgttgggg aggcggttgg gggcgaggac
     2641 gaggccgggg gacgggattt aaagtccctg tcattcccca aattcagacc ggctccggcc
     2701 gcccctgcca ggggcttgaa tcgttggggg cggcgacgca gggttccggc ctgcggcctc
     2761 caccctgcgc ttgattccgg ttgtccgctt cgcggacttt aggccggtca acaagtcaga
     2821 accgcctgca ttgacaccgc ctgcattgac gttggcatcg atagtcggct tgcgatttgg
     2881 ggaatgacaa gatttagagt cgggagccga agagggcgct gccgaggcgg acgagggtgg
     2941 cgccttcctc gatggcgacc tcgaaatcgc cgctcatgcc catcgagaga tgtttctttt
     3001 caaagttggg cggtccctgg gcctcctcga ggaggcggcg cagggcggag aaatgcgggc
     3061 gcgtccgttc gggatcgtcg tcccagggcg gaatcgtcat caggccgagc gcgcggagat
     3121 ggggggccgc ggcgagcgcg gcgaggaggg ccgggaggtc ggcgggggcc gcgccgaact
     3181 tgctggcctc gcccgccacg ttgagttgca gcaggatggc ctgctgggtg tcggccgcgg
     3241 cggcgcgccg ttcgagttcc tcgatcaggc gcgttgagtc gaccgagtgg atcaattcga
     3301 agcggccgac ggcggcggcg gccttgttgc gttggaggtg accgatcagg tgccagcgcg
     3361 gaccggggcc gaccgcgtcc attttcggga gggcttcctg gacgcggttc tcgccgaaga
     3421 gcgactggcc ggcgtcgatg gcggcgcgga cggcgtcggc gggatgggtt ttggtgacgg
     3481 cgacgagctc gacggcggcg gggtcgcggc cggcgcggcg cgcggcgcgt tcgatgcgtt
     3541 cgcgcaccgc ggcgaggcgt tcgcgcaggt cggcgggggg attattggtt tgcctgccca
     3601 aatcggacat tgctacgatg ccttgagact ggatgacttg cctccggggc tcccatggac
     3661 tcgccgaccg cgccacgctc gaagctaaca gccctcctgc cgactttcaa cgaagaggcc
     3721 catctcgccg ggtgcatcga ttcggtggcg tgggccgatg aaattctggt cgtggattcg
     3781 ttcagcacgg atgcgacgcg cgagattgcg cgggcccggg gggcgcgcgt cgtggaacac
     3841 gaatacgtca actcggccgc gcagaagaac tggtcgatcc cgcagtgttc gcacgagtgg
     3901 gtgctggtcg tggattcgga tgagcgggtg acgccggaat tgcggcgcga gatcgagggg
     3961 gtgctgacgg ccgacgcgaa cgggcggccg gtggccgacg gttaccggat cgggcggatc
     4021 aatcattttc tcggccggcg gattgagcac tgcggctggc agaacgacac ggtgctgcgg
     4081 ctgttccggc gcgacaaggg gcgctaccag gagcgggagg tccacgccga cgtcctggtg
     4141 gaggggaaga tcgggcgact gcggcacaag ctgctgcact atacgtttcg cgacttcgag
     4201 cagtatatgc gcaagttcga ccggtatacg tcgtgggcgg cgggtgatcg gggccggcgg
     4261 acgccgcgcg tggggtatac gcatctgctc ctgcggccgc tgggccggtt tgtgaagcag
     4321 tatgtgttga agcgggggtt tctggacggg cgggtgggtc tggtggtgtg cctgctcgcg
     4381 tcgtacagcg tgttcatgaa gtatgcgaag ctgctggagc ggcaattgca ggcggaaggg
     4441 ggcggcgccc cgggtgagag cgaggcggcg gcgcgggccg agggcgcggc ggcgtcgctc
     4501 gatatgaagg catgaccgta tccgtcgcgc caccgccgct gccgccttcg gccgaccggg
     4561 tggatggcgc cgagcttccg ccggggcagg cggcgccggg gagtccgctg gaacggctgc
     4621 tgcgccgcca gcgcgcggag tcgctgcgcg tcttcatcaa gggggtcaac tgggtgggcg
     4681 acgcgatcat cgcgacgccg gcgctggccc atctgcggcg ccagtttccc gatgtgcaga
     4741 tcacgctgat ggtccggccg tgggtggcgg cgatttatga gaattcgagc gacatcgacg
     4801 agctgtgggt gcatgacgac agcaaggatc tgaacgcatt tgtgaaggcc gtgcggatgg
     4861 tgcgcaaggg ccggtttcag atcgggatcg cgctgcccaa ctcgctgcgg tcggggctgc
     4921 tgctgcggct ggcgtcggtg ccgtatcgat tcggctacca cacgagtctg gcgcggcgga
     4981 tgttgctcaa ttacggccag acgccggcca gcgatctact ggcggggcat caggttttct
     5041 actacttggg gattattgaa gggatggtgg gccaggcggc gcgtcgccag cagttgcggc
     5101 tgacggtggg ggaactggag cgcgaggaga tccggcggct gctggcgcag cgggggctgg
     5161 accggggccg gccgatcgtg gggctcgcgc cgggttcgat caattcgcaa gccaagcgct
     5221 ggccggccga acggtacgcg gagctggccg atctgctggg gcgcaagacg gccgccgaga
     5281 tcgtgctgct ggggagtggg aaggagaagg aggtctgcga ccgggtggcg acgctgtgcc
     5341 atcagccggt acacaatctt gcgggcgagc tgaacctggg ccaggtgatc gcgctgatgg
     5401 aacggctgca ggcgctgatc tgcaatgatt cgggggcgat gcacatcgcg gcggcgctgc
     5461 gggtgccgac gctggcgatc ttcgggccga cggaatggac gacgacgtat ccgttttcca
     5521 acgcggccaa catcatccgc aaggaggggg tggcgtgcgc gccgtgcatg ctgcgcgaat
     5581 gcccaatcga tcaccggtgc atgacgggga ttgcggtggg ggacgtgctg gacggtttcc
     5641 gcaaggtgct gcagcaggcg aagcagtacg gccgctgaag ggcaggcggg ggatgaagcg
     5701 gcgccgcggc gggcggaaga tctgttggta aaggcggcgc cattggttac gattgcaggg
     5761 caatgaacag cgacgagcct ccgagtaccg tcaaccagcc cgcgccccgc gtcgtgcatc
     5821 tggacgacgg ccggctgtgg cgcggcggcc agcatcaggt ctggctgctg ctgggggaac
     5881 tggcgcggcg cgggatcgcg cagatgctgg tggcgcacga cggctgcccg ctgacgctgc
     5941 gggcgcgcga ggcggggatc gaagtggaga cggtgcattt tcggggggaa tgggactggc
     6001 ggacgccgcg gcggattgcc gcgctggccg acgcgttcgg ggcgaacctg ctgcacgcgc
     6061 acacgagcca tgcgcacacg ctgggactgc gcgctttgcg gcagatgcgc cagcggggga
     6121 tgctggtgac gacgcggcgg gtggatttcg cgatccggcg caacatcctg tcgcgccgca
     6181 agtacaagtt gccgggtcag catttcattg cgatctcgcg ggcggtgcgc gacgtgctgg
     6241 tggcgggtgg cgtcgcgccc gagcggatcg acgtggttca cagcggcgtt ccgccgattg
     6301 cggccgagaa gctggagccg cgcgcggcgg tgcgggcgga gctggggctg gcgtcggacg
     6361 agattgcgat cgtcaacatc ggggcgctga ccgatcacaa gggccagcgc tggctgatcg
     6421 aggccggccc ggaggtgttc cgggcatttc ccagggcgcg gatccatata ctgggggagg
     6481 gggagttgcg tccggcgctg gagcagcgga tccgcgaact gaatctgcgc gggcgggtga
     6541 ttctgcacgg ccacgtggag aacgcgcggc tcaagctggc ggcgtttgat ttgtttgtgt
     6601 cgagctcgca cctggagggg ctcgggacgg tgaacctgga cgcgatgctg gcgggggtgc
     6661 cggtggtggc ggcggcggcc ggcggcgtgc cggagattgt ggaggacggg cggacgggtc
     6721 ggctggtgcc ggcgcgggat gccggcgcgc tggcgcgggc gatcgtggag gcgctgagca
     6781 acccggcgga gacggccgcg atgattgaac gcgggcgccg gcacgtggaa gagcattttt
     6841 ccgcggcctc gatggcggcg gggaccctgg cggtgtatcg gaaattgctg gcggcgcatg
     6901 cggccgcggg gtttgtggcg ttgggtcggt gatggtgggg cgggcattcc ccaggttgtg
     6961 ctgcgctcca cccggggcga ttcaactgcc cctcttcgag gcggcattct ggcatccctg
     7021 cgggatgcgg gggaggggag gatcggggag ggggaaagag ggagcggtgg cggtggagtg
     7081 gcgattgcga taccgatacc gatgccgata ccgattgtat gattcatcac ggaagacacg
     7141 tatgggcagg aaacggggga gaaccggcga gggcggcggt cgcacactgg cggggatggg
     7201 tccggactgg tgatgacgag ggtgcgacat tggccgggat ttcacggatt cggcttggca
     7261 gggtccggcg cggctggcaa gatgggcggg agactccagg aagcgacgcg gcccgatgag
     7321 caaggaaccg attctgctat ttggacttcc gcgatcgggg acaacgtggc tgggcaagat
     7381 tttcgacagt catcccgcga cgttttaccg gcatgaaccg gacagtctga agcggttcgg
     7441 gacggtgccg ctgatcgtgg acgcgggggg cgcggcggcg tggcgcgacc aggtggggcc
     7501 gtatgtcgag cagatcgcga cgatgcggtc ggccaagctg tggaagtatc cgttgttccc
     7561 gaagcactac tgccgcgggg gtcggtttgc gctgctgcgg gcgagcgtgt ttgccggcaa
     7621 gtcgatggtg aaagtgttcg gcgattttcc ggtgccgaat tttgtgaggg cggaccagcg
     7681 cggcgacatc cggctggtct ggaagtcgat cgagtcggtc gggcggctgg ggttgattgt
     7741 gcggacgctg ccggcggtgc gcgtgatact gattttgcgt catccgtgcg ggttcgtcgc
     7801 gtcgatcctg cgcggcgagg cggcaggcaa gttcggccgg ggctgggcgc tgagtcacga
     7861 cctgacgatc atggagaaac tgaccgcgtg cgagcaggcg cggcgccggg ggattacggt
     7921 ggaacggatg caggcgatgg agccgatcga gcggctggcc tggcactggg ccttgagcaa
     7981 tgagaaggcg atggaggaga tcgaggggcg cgacgattgc atggtgctgc gctacgagga
     8041 cgtgtgcgcg cacccggtgg aggagtcgcg gcggctgttc gcgtttgccg gtctggcctg
     8101 ggacgcacag acggaggaat ttctggcgcg cagcacggcg agcgagcaga acgattacta
     8161 cagcgtgttc aagaatccgc tggtggcggc gaacaagtgg cgcaatcagc tctcggtgca
     8221 gcaagtggag caggtgctga gcatcgcgcg gcagagcgtg ccggggcggc tgtatgattc
     8281 gtggaagttc gacgaagcgt cgaagacggt gccgtcggcc cggccgctgc aggtgtccag
     8341 gtaatttctt tgagcgagtc gatgagaaga gagaaccggc ggatgcgagc gatccgacgg
     8401 aaggggtcgg agtgcgtcag cgccagagtt gccagtcgtg cgcggaggag cgggtgagga
     8461 tcgtgcggta gccgaggtcc tcgagaatga agggatgcaa gggacggaag ggacgtgagg
     8521 gacggaaggg acgcgaccgt tcaatttcga gagccgagga cgagagacgg gagccgagag
     8581 ccgaattctg gggagggccg ttgggggcga tgatctgacg ccgagaggcg gaggggcgcc
     8641 gatgaggtgg atgggtcgtg gatgggatag gttgggcgcg aacgggggca acgccactca
     8701 ggggcgtggg gctcccaggt tgcggccggt tgcttgttgc ccggaggcgt cggggcacga
     8761 ggggggcgag gacgagggac gaggccgagg gaccctattt gagtgaggac gagtcgctta
     8821 tgaaccggcg attcccaacg aagggactga attgggcagg ccttcgggag cgatcacgag
     8881 gacgaggaag aggaagcggg aggacggtag ataggggttg cgggcggggc tggggggtaa
     8941 tatgatgaac ggacccggat atccgctttt ccggggtgag ttcctgccgg gggggattca
     9001 tgaagcgcgc cacgccagcc gccgagaatg tcagcccgag ttcctcccac gcggaatccc
     9061 ccgctttgca gcagagcgaa aaagctgatc gggcgggttc ctccggggcg gaatccatgg
     9121 gcgacggaaa ttcgagcccg gtccgcgagc ggcgggcgcc ggcgctgatc agcgggaacg
     9181 aggcgctggc gctggcggcg cgcgacgcgg ggatcgcgct ggggacgggc tatcccggga
     9241 cgccgtcgac cgagattctt gaggagctgg accggcaggg ggggcgggcg cagtgggcgc
     9301 ccaacgagaa agtggcgctg gaggtggcgc tgggagcggc gtattgcggg gcgcgcgcgc
     9361 tggtgacaat gaagcatgtc gggctgaacg tggcggccga cccgctgttc acggcggcct
     9421 acacgggcgt ggcgggggcg ctggtggtgg tgacggccga cgacccgggg atggcgtcga
     9481 gtcagaacga acaggacaac cggcactacg cggcggcggc gcggttgccg atgctggagc
     9541 cggccgattc gcagcaggcg tatgaatttc tgctgacggc ggtgtggatt tcggaacggt
     9601 gggggatacc ggtgctgctg cggccgacga cgcgggtgtg tcacggcaaa tcgatcgtgc
     9661 ggttcgagcg gggggcgccg gcggcgccgg cggcgcggta tgagaaggac gtgcggagcc
     9721 gggtgatgat cccggcgcat gccggaccgg cgcaccgcgc gctggaggcg aagctgaagg
     9781 cgatcgcggc gtggaatgac gagcgcggtc cgaaccaggc ggtgtcgcgg ggggtgtcgg
     9841 gggcgccggc gatcatcgcg tcggggatcg cggcgatgca cgcgctggag gcggcgccgc
     9901 aggccgacgt gctgatgctg gggatgacgt atccgccgcc gctgggtctg atccggcgct
     9961 tcgcggcggc ggcggcggcg gaggggcacc cgctgcatgt gatcgaggaa ggggatccgg
    10021 tgctggcgga gcggctgcgg gcggcggggc tgaacgtcca gtcggcgccg gaagactacc
    10081 gtttcggcga gctgaacgtc aatcgggtgc gggcgatcct gagcggggag acggcgccga
    10141 cggcggcgac gccggcgggc cagccgccgc ggctgtgcac ggcgtgcccg caccggtcgg
    10201 tgttcagcgt gctgcgcgat ctgaactgca tcgtggcggg agacatcggt tgttacacgc
    10261 tgggggtgtt gccgccgtac gaggcgctgg acacgtgcgt gtgcatgggg gcgagcatcg
    10321 gggtggggct ggggatgcgg catgtgctgc cgcgggagga ggcgaagcgg gtcgtcagcg
    10381 tgatcggcga cagcacgttc atccacagcg ggctgacggg gctggtcgag atggtctaca
    10441 acccgccgcc gacgggacac gtggtgctga tcctggacaa cgggacgacg gcgatgacgg
    10501 ggctgcagga gcatccggga acgggccgtc ggctggggca tgagccgacg gcgcgggttg
    10561 atttcgagga agtgatccgg gcgattggaa tccgcgaagt gcatgtgatc gacgcgacgg
    10621 aggatgtcca ggcgtttgag acgctgctgc gggtgtgtct ggacggcgag cagccggcgg
    10681 tgatcatcgc gcggcggccg tgcatgctgg cggcggggcg catccggcgg tacgagaagg
    10741 ccgcggcgga gacggcggcg ggcgcgtgcg gatgcgcgga aggggaggag cggtgatggg
    10801 cgcgagcggg gcgggcggcg gcggcggggt ggtgagcgtg gtgatcgccg ggctgggggg
    10861 ccagggggtg ctcaaggcct cggacatcct gagtgaggcg gcgttcgcgg ccgggctgga
    10921 tgtgaagaag agcgaagtgc acgggatgag ccagcgcggg ggatcggtgg cgagcgacgt
    10981 gcgcttcggc gcgaaggttt acagcccgat ggcgccggtg ggggaggcgg actacctggt
    11041 ggtgctggcc gaggatcagg tggaagtgtt ccggcatcgg ctgtcgccgc ggggggagc
//
LOCUS       DAOPCO010000123        11086 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5763_length_11086_cov_5.305049, whole genome shotgun sequence.
ACCESSION   DAOPCO010000123 DAOPCO010000000
VERSION     DAOPCO010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5763_length_11086_cov_5.305049"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1220)
                     /locus_tag="PLS90_09750"
     CDS             complement(3..1220)
                     /locus_tag="PLS90_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02725.1"
                     /translation="MNEPLGHDPRLRATLLRIGRLGRLLAGGALIPLVIGFFLNPVAL
                     AQAWLVGFSFWLELSLGGLALLMLQYQITGRWGFFSRRLLEAIALTLPLLAVLFIPVI
                     LSLHALYPWTDPELVAASRVLSAKAGYLNIPFFTIRATLYWAGWIALALRLRFWSRRL
                     DQPRFAPAAAVRLARLSAGGLVLWFLTFSFAAVDWWMSLEPEWFSSIYSVILMMGQAL
                     IAAALVTFALIALRRRPPLAGLLDRPVLNDLGNVILAFTMLWTYMAFMQWLIIWNGNL
                     PEDISWYVRRTRNGWQWFVAALFLLHFAAPFVVLLSRTAKRNARVLGGLALLLLAAHL
                     ASIWWLIRPAWGTRFHWLDPIIVIGIGGAWIWLAIRRYLAAPPLPLHDQRFSRLLHDR
                     PPAPALADPSPAP"
     gene            complement(1217..1798)
                     /locus_tag="PLS90_09755"
     CDS             complement(1217..1798)
                     /locus_tag="PLS90_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HPK02726.1"
                     /translation="MKRPFRKALLLMLLVAPVVSCRQDMYDEPRYEPLESSPLFDDGA
                     SARPLPPGTVPVGHLRDDDHLDRGLVAGEPAPDLPLTLTPELLARGRRQFNTFCSPCH
                     GAAGYGDGMVARRGFPPPPSFHQPRLRAATPGYLFDVITNGYGIMYSYAARLKPKDRW
                     AVAGYVKALQLSQHARLDDVPESERPRLTEGES"
     gene            complement(1795..2382)
                     /locus_tag="PLS90_09760"
     CDS             complement(1795..2382)
                     /locus_tag="PLS90_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HPK02727.1"
                     /translation="MTGDPRREGYPLYGLLAEFDAPAPLLNAVQRARAAGYAAMDAYA
                     PFPVDGLAEALGRRRDLVPWFVLAGGVLGGVGAYAMMAYSSIIDYPLNVGGRPLHSWP
                     SFIPITFELTVLLASLSGVAAMLLLNGLPRPHHPVFNVPEFARASRDRFFLCIESADA
                     RFDPETTRDFLATLNPLQIHAIENTEAAPREAPTQ"
     gene            complement(2379..3761)
                     /gene="nrfD"
                     /locus_tag="PLS90_09765"
     CDS             complement(2379..3761)
                     /gene="nrfD"
                     /locus_tag="PLS90_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPK02728.1"
                     /translation="MSAAAGQPPEPDGLALLGPGVTAASVTDHVAGIVIARPSPRFWW
                     LAFGIGFLLMMLLLASIAWILVRGVGVWGVNVPVAWGFAIVNFVWWIGIGHAGTLISA
                     ILLLMKQTWRNSINRFAEAMTLFAVACAGMFPLLHLGRPQVFYWLFPYPDTMNLWPQW
                     RSPLVWDVFAVTTYGIVSLVFWYVGLVPDLATIRDRADQPWKQRLFGIFALGWRNSAL
                     HWRRHQSLYLLLAALATPLVASVHSIVGLDFAISIVPGWHSTVFPPYFVAGAIFSGFA
                     MVLTLIIPIRAVYGLHAYITGRHLDFMARVMLATGLMVSYGYLSEAFFAYYSGSRYEK
                     YMMLNRALGPFAPAFWALILCNVAAIQFLWLGRVRRGPLALFLISIVINIGMWLERFV
                     IVITSLHRDYLPSAWGDYNATRWDWATFLGTLGFFLACMFLFIRFVPLLSIYELREMV
                     HARAEHGGNA"
     gene            complement(3758..6733)
                     /locus_tag="PLS90_09770"
     CDS             complement(3758..6733)
                     /locus_tag="PLS90_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HPK02729.1"
                     /translation="MTAAEAHPAPRRPPTSMHGRDNWTSLADLARSPEFRDRIEREFP
                     RHAALWDSGLDRRRFLELAAASLALGGLSACVQQPPEPIVPYVIQPELVTPGIPLYFA
                     TTLLRDGLGIGAIAESHEGRPTKIEGNPNHPASLGAADAIMQAEILTLYDPDRAATVL
                     REGRIAAWADFTAWLHGESAAWRPSAGRGLRLLTGPISSPSLLALIARWLEQFPAARW
                     HVHSPLRPAAALEPFAPLYHVERAERIVALDCDFLLNGPASLAHARRFIDGRRVRADR
                     PRMNRLYALESRPTLTGANADQHWTLPPPALELAVARLAALVGVPGAAPALDWPAGAD
                     WLPIVARDLLDHPARSLIVADERLSPDARALSNAINQRLGAAGATLDWIPNPAPPPED
                     GSLAALAEAMRAGEVDTLFILDCNPVHTSPADFNFADLLRRVRRRVCLGLYHDETAAL
                     CHWHIPRAHELESWGDARAFDGTISTIQPLIAPLYGGRTPLELLALMTGENGMSDYDR
                     VRAHWAAAAPGADFDARWRRWLHDGVFEGTALPAAAGPAPADPAAALAALERRRADAV
                     PARLHLLFEPDPALLDGRYANNGWLMELPRPITKLTWDNAALIAPATARRLGLASRQV
                     VELTLRGRSLRVPVWIVPGHAADCVTLPFGFGRERAGRVGDGVGVDAYRLRFADTPWW
                     TAGLELTPTNERYPLACTQDHFRMHERAPVREATLAEFIQTRGEIAPGHGDPPAQEPS
                     LLPEFPYDGCKWGMAIDLTACIGCNACVAACQAENNIPVVGREQVALGREMHWLRIDR
                     YYTGDLHQPDTAFQPLACVHCEHAPCEVVCPVAATVHDAEGLNLMVYNRCIGTRYCSN
                     NCPYKVRRFNFLQYTDRVTESLKLMRNPEVTVRNRGVMEKCTYCIQRISRARHAALRE
                     GRAIGADDVHTACQAACPTRAITFGDLNHPGAEVNQWKAEPHNYDLLGDLNTRPRTSY
                     LARIRNPHDELDADRSQSG"
     gene            complement(6748..7398)
                     /locus_tag="PLS90_09775"
     CDS             complement(6748..7398)
                     /locus_tag="PLS90_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPK02730.1"
                     /translation="MPQLFPPVANVLARWSLAALLVFLGAGAWAWTAFTRSDYLTRVN
                     HAPAQPVPFSHQHHVGRIGIDCRYCHASVADSAFAGLPPTHTCMSCHSQLWRDAPMLD
                     PVRRSLAAGVPLRWIRVYDLPDFVYFDHSVHVRQGIGCTTCHGPVGEMPLTRKVADLS
                     MRWCLNCHAKPERYVRPREEVFNPDYTPPADQQALGARLVREYGIQRRIDCSTCHR"
     gene            complement(7458..9308)
                     /locus_tag="PLS90_09780"
     CDS             complement(7458..9308)
                     /locus_tag="PLS90_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPK02731.1"
                     /translation="MSPRRPTPDSPPIGDHGVIGDLNTVALVAMDGTIDFMCFPSFDS
                     PTLFAALLDHARGGAFRLAPRFAAAQRKQMYLPDTCILLTRFMSADGVAELCDFMPIE
                     EMGHAHDLIRRVRTIRGEVEFELVFDPRFDYGRAAHRVEKHGATLLFIPDAPNLPTVR
                     LRSTIPLAVRRGRALSRFTLKAGQSVDVILEELQDEDESPSSAPDYADRAFQETIDYW
                     RIWLSRSSYHGRWGEMVNRSALTLKLLTFRPTGALLAAPTFGLPETSDHRRNFDYRFT
                     WIRDASFTVYAFLRLGFTDEAASFIDWLSHRCDEIAPDGSLRVIYGIHGRLEPAEREL
                     TPLRGYRGAGPVRIGNADPAPNQIDIYGELLDSIYLYNKFGAPIADATWRKVTRHVEY
                     VCAHWRDPDRGIWDLPGEPHEYAYSRLMCWVAVDRAIRLALKRSFPAPLDRWREIRDA
                     IYNDLMENFWDTQRQSFIQAKDSPALDAACLMMPMVKFVGPTDPRWIGTLRAIERHLA
                     EDALVYRHRYPDGAPPDAVDQPLASFSVCSFWYAECLSRMGELDRARLMLEKMFTYSN
                     HLGLFSAEIGPNGELLGCFPQALTHLGLISAAYDLNRRLSRAGASATGLE"
     gene            complement(9305..10471)
                     /locus_tag="PLS90_09785"
     CDS             complement(9305..10471)
                     /locus_tag="PLS90_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPK02732.1"
                     /translation="MRAIGVFPRSRQIDLVELDEPLRRGRHDVKVRMLEVGLCGTDRE
                     IVSFQYGAPPPGSSFLVIGHEGLGLVADVGPAVAALRPGDLVIPMVRRPCPHAHCRPC
                     RAHRADFCYTGDFTERGIKAAHGFTAETVVDPEQFFIPVPSELRHIAVLTEPLTIAEK
                     ALTQVWDVQERLPGPSRHKSDRATAAARLAGDLGQSDQGFHHRAVVLGAGPVGLLGAM
                     LLVLTGFETTVYSREPAGGPKARLAQQIGARYLSAADADPPDLARAVGNIDLVYEATG
                     AAEVSFALLRELGANGVFVFTGVPGRKHPIQCDAAAIMQNLVLRNQVVLGTVNAGRDA
                     YRAAVDDLGRMARRWPGVLQQLITARHPLEDFRRPLLDHPAGIKHVLVHGADGS"
     gene            complement(10607..10756)
                     /locus_tag="PLS90_09790"
                     /pseudo
     CDS             complement(10607..10756)
                     /locus_tag="PLS90_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
BASE COUNT         1928 a         3111 c         4461 g         1586 t
ORIGIN      
        1 cctcaggggg cgggggatgg gtcggcgagg gctggcgccg gcgggcggtc gtgcagcagg
       61 cggctgaatc gctggtcgtg cagggggagc ggcggcgcgg cgaggtagcg gcgaatcgcc
      121 agccagatcc aggcgccgcc aattccaatg acaatgatgg gatcgagcca atggaaacgc
      181 gtgccccatg ccggtcggat cagccaccag attgaggcca gatgcgcggc gagcagcagc
      241 agggcgagac cgccgaggac gcgcgcattg cgtttggcgg tgcgcgaaag gaggaccacg
      301 aagggggcgg caaaatgcaa tagaaacagc gcggcgacga accattgcca gccgttgcgc
      361 gtgcgacgca cgtaccaact gatgtcttcc ggcagattcc cattccagat aatcagccac
      421 tgcatgaagg ccatatacgt ccagagcatc gtgaatgcga ggatgacgtt gcccagatcg
      481 ttgagaaccg gcctgtcgag caggccggcg aggggcggcc ggcggcgcag cgcgatcagc
      541 gcgaatgtga ccagcgccgc ggcgatcaga gcctggccca tcatgaggat gacggagtag
      601 atcgaggaga accattcggg ctcgagcgac atccaccagt cgacggccgc aaaggagaag
      661 gtcaggaacc agaggacgag gcctccggcg ctgaggcggg caaggcgcac ggcggcggcg
      721 ggggcaaacc gcggttgatc gagccggcgc gaccagaagc gcagacgcag ggcgagagcg
      781 atccagcccg cccagtagag ggtcgcgcgg atcgtgaaaa aggggatatt caaatagccg
      841 gcctttgcgg agaggacgcg gctggcggcg acgagttcgg gatcggtcca ggggtaaagg
      901 gcgtggaggc tcagaatgac tggaatgaag agcacggcca gcagggggag ggtcagggca
      961 atcgcctcga gcagccggcg ggagaagaaa ccccagcggc cggtgatctg atattgcagc
     1021 atgagcaggg cgaggccgcc gagggagagt tccagccaga acgagaagcc gacgagccac
     1081 gcctgggcga gggcgacggg gttcaggaaa aatccaataa ctaatggaat taacgcgccg
     1141 ccggccagca atcgaccgag gcgtccgatg cggagcaatg tcgcgcgcaa gcgaggatcg
     1201 tggcccagcg gctcgttcac gattcaccct ccgtcagccg cgggcgttcg ctttcgggca
     1261 cgtcgtccag ccgggcgtgc tggctcagtt ggagggcctt cacatagccg gcgacagccc
     1321 agcggtcctt gggcttgagg cgcgcggcgt agctgtacat gatgccgtag ccgttcgtga
     1381 tgacgtcgaa gaggtagccg ggggttgcgg cgcgcaggcg gggctggtgg aaggaaggag
     1441 gcggcggaaa gccgcggcgc gccaccatgc cgtcgccgta gccggcggcg ccgtggcagg
     1501 gggagcagaa ggtgttgaac tggcggcggc cgcgggcgag caactcgggc gtgagggtca
     1561 gcggcagatc gggggcgggt tcgccggcga cgaggccgcg atcaaggtgg tcgtcgtcgc
     1621 gcaggtggcc gaccgggacc gtgccgggcg ggagcgggcg ggccgacgcg ccgtcgtcga
     1681 acaaggggct tgattcaaga ggctcgtagc gcggctcgtc gtacatgtcc tggcggcagg
     1741 agacgaccgg cgcgaccaat agcatcagca ggagggcttt tctgaatggc cgtttcattg
     1801 ggtgggggcc tcgcgcgggg cggcctcggt gttctcaatg gcgtggattt gcagcgggtt
     1861 gagcgtcgcc agaaaatcgc gcgtcgtctc gggatcgaag cgggcgtcgg ccgattcgat
     1921 acacaggaaa aaacggtcgc gcgacgcgcg cgcgaattcg gggacattga atacggggtg
     1981 gtgcgggcgc ggcaggccgt tgagcagcag catcgccgcc acgccggaga gcgaagcgag
     2041 caggacggtc aattcaaatg taatgggaat aaaggacggc cagctatgga ggggccggcc
     2101 gccaacgttg agcgggtaat cgatgatgga cgaataggcc atcatcgcgt aggcgccgac
     2161 gccgcccagc acgccgccgg cgagaacgaa ccacggaacc aggtcgcgcc ggcggccgag
     2221 ggcctccgcc aggccgtcga cgggaaaggg ggcgtaggca tccatggccg catagccggc
     2281 cgcgcgggcg cgctggacgg cgttcagcag cggcgccggc gcgtcgaatt cggccagcag
     2341 gccgtagagc ggatagccct cgcgacgcgg gtcgccggtc atgcgttgcc tccgtgttcc
     2401 gcgcgggcgt ggaccatttc gcgcaattcg tagatcgaga gcagcgggac gaagcggata
     2461 aagaggaaca tgcaggcgag gaaaaagccg agcgtgccga gaaaggtcgc ccagtcccag
     2521 cgggtggcgt tgtagtcgcc ccacgccgag gggaggtaat cgcggtgcag gctggtaatg
     2581 acaatgacaa agcgctcgag ccacatgccg atgttaatca caatggaaat caggaagagc
     2641 gccagggggc cgcggcggac gcgtccgagc cagaggaact ggatcgcggc gacattgcag
     2701 aggatcagcg cccagaacgc cggagcgaac gggccgagcg cgcggttgag catcatgtat
     2761 ttctcgtagc ggctgccgct gtaataggcg aaaaaggcct cgctgagata gccgtaggac
     2821 accatcaggc cggtggcgag catgacgcgc gccatgaagt cgaggtggcg gccggtgatg
     2881 taggcgtgca ggccgtagac ggcgcggatg gggatgatga gggtgaggac catggcgaaa
     2941 ccggagaaaa tcgcgccggc gacgaaatag gggggaaaga cggtgctgtg ccagccgggg
     3001 acgatggaga tggcgaaatc gaggccgacg atgctgtgga ccgaggcgac gaggggggtg
     3061 gccagcgccg cgaggaggag gtacagcgac tgatggcggc gccagtgcag ggcggagttg
     3121 cgccagccga gggcgaaaat gccgaacagc cgctgcttcc agggctggtc ggcgcggtcg
     3181 cgtatcgtcg ccaggtcggg caccaggccg acataccaga agacgaggga gacaatgccg
     3241 taggtggtga cggcgaagac atcccagacg agcgggctgc gccactgcgg ccagagattc
     3301 atcgtgtcgg ggtaagggaa aagccaatag aaaacctgcg gccggccgag gtggaggagc
     3361 gggaacatgc cggcgcaggc gacggcgaag agggtcatcg cctcggcgaa gcgattgatg
     3421 gaattgcgcc aggtctgctt catcaggagc aggattgcgg agatcagggt gccggcgtga
     3481 ccgatgccga tccaccagac gaaattgacg atggcgaagc cccaggcgac cggcacattg
     3541 acgccccaga cgccgacgcc gcggacgaga atccaggcga tcgaggcgag gaggagcatc
     3601 atcaggagga agccaatgcc gaaagccagc caccagaagc gcggggaagg gcgggcgatg
     3661 acgatgccgg cgacatgatc ggtcaccgag gcggcggtga cgcccgggcc cagcagggcc
     3721 aggccgtcgg gttcaggggg ctggccggcg gcggcgctca tcccgattgg ctccggtcgg
     3781 catccagttc gtcgtgtgga ttacgaatgc gcgcgaggta ggaggtgcgc ggtcgcgtgt
     3841 tcaagtcgcc cagcaggtca tagttgtgcg gttcggcctt ccattggttc acttcggcgc
     3901 cggggtggtt caggtcgccg aaggtgatcg cgcgcgtggg gcaggcggcc tggcaggcgg
     3961 tgtggacgtc atcggcgccg atggcgcggc cctcgcgcag ggcggcgtgg cgcgcgcggc
     4021 tgatccgctg gatgcagtag gtgcattttt ccatgacgcc gcggttgcgc acggtgacct
     4081 cggggttgcg catcagcttg agcgattcgg tcacgcggtc ggtgtattgg aggaaattga
     4141 agcggcggac cttgtagggg cagttgttcg agcaatagcg cgtgccgatg cagcggttgt
     4201 aaaccatcag gttgagccct tcggcgtcgt gcacggtggc ggcgacgggg cagaccactt
     4261 cgcagggggc gtgttcgcag tgcacgcagg ccagcggctg gaacgccgta tcgggctggt
     4321 gcaggtcgcc ggtgtagtag cggtcgatgc gcagccagtg catctcgcgg ccgagggcga
     4381 cctgctccct gccgacgacg gggatgttgt tctcggcctg gcaggcggcg acgcaggcgt
     4441 tgcaaccgat gcaggccgtc aggtcgatcg ccatgcccca cttgcagccg tcgtagggga
     4501 actcggggag aagcgagggt tcctgggcgg ggggatcgcc gtggccgggc gcgatttcgc
     4561 cgcgggtctg aatgaactcg gccagggtcg cctcgcggac gggcgcgcgt tcgtgcatgc
     4621 ggaagtggtc ctgcgtgcag gcgagcgggt agcgttcgtt ggtgggcgtc agttccaggc
     4681 cggccgtcca ccagggggtg tcggcgaagc gcagccggta ggcatcgacg ccgacgccgt
     4741 cgccgacgcg gccggcgcgc tcgcggccga agccgaacgg gagcgtgacg cagtcggccg
     4801 catggccggg cacgatccac acggggacgc ggagcgagcg gccgcgcagc gtgagctcga
     4861 cgacctggcg gctggcgagc ccgaggcggc gcgcggtggc cggcgcgatc agcgcggcgt
     4921 tgtcccaggt aagcttggtg atcgggcggg gcagttccat gagccagccg ttgttggcgt
     4981 agcggccatc gagcagggcg gggtcgggtt cgaaaaggag gtgcaggcgc gcggggacgg
     5041 cgtcggcgcg gcgccgttcg agggccgcca gcgcggcggc cggatcggcg ggggcggggc
     5101 cggccgccgc cggcaaggcg gttccctcga agacgccgtc gtggagccag cgccgccagc
     5161 gggcgtcgaa atcggctccg ggggccgcgg cggcccagtg cgcgcgaacc cgatcgtagt
     5221 cgctcatgcc attctcgccg gtcatcaggg cgagcaattc cagcggcgtc cggccgccgt
     5281 agagcggcgc gatcaggggc tggatggtcg agatcgtgcc gtcgaaggcg cgggcgtcgc
     5341 cccatgactc caactcgtga gcgcgcggga tgtgccagtg gcaaagggcg gcggtttcat
     5401 cgtgatagag gccgaggcag acgcggcggc ggacgcggcg cagcaggtcg gcgaaattga
     5461 aatccgcggg cgaggtatgg accggattgc aatcgaggat gaacagggtg tcgacctcgc
     5521 cggcgcgcat ggcctcggca agcgcggcga ggctgccgtc ctcggggggc ggggcggggt
     5581 ttgggatcca gtccagcgtg gcgccggcgg cgcccagccg ctgattgatc gcgttggaca
     5641 gggcgcgcgc atcggggctg aggcgctcgt cggccacgat gagcgagcgg gcggggtggt
     5701 cgagcaggtc gcgggcgacg atcggcagcc agtcggcgcc ggccggccaa tcgagcgcgg
     5761 gcgcggcgcc ggggacgccg acgagcgcgg ccaggcgggc gacggcaagc tccagcgcgg
     5821 ggggaggaag ggtccaatgc tgatcggcgt tggcgccggt cagggtgggt cggctctcca
     5881 gggcgtagag ccggttcatg cggggccgat cggcgcgaac gcggcggccg tcgatgaagc
     5941 gccgcgcatg ggccaggctg gcggggccgt tcaggagaaa gtcgcagtcg agggcgacaa
     6001 tccgctcggc ccgttcaacg tggtagaggg gggcgaaggg ttcgagggcg gcggccgggc
     6061 ggagcggcga gtggacgtgc cagcgcgccg cggggaattg ctccagccag cgggcgatga
     6121 gtgccaggag cgagggggag gaaatcggcc cggtgaggag ccgcaggccg cgcccggccg
     6181 aggggcgcca ggcggccgat tcgccgtgga gccaggcggt gaaatcggcc caggcggcga
     6241 tgcggccctc gcgcaggacg gttgcggcgc ggtccggatc atacagggtc aggatttcgg
     6301 cctgcatgat tgcgtcggcg gcgccgaggc tggccgggtg gttgggattc ccctcgattt
     6361 tagtggggcg gccctcgtgg ctttcggcga tcgcgccgat gccgaggccg tcgcgcagga
     6421 gggtcgtggc gaaatagagc ggaatgccgg gcgtgacgag ttcgggctgg atgacgtagg
     6481 ggacgatcgg ttcggggggt tgctggacgc aggcggagag gccgccaagg gcgagggagg
     6541 ccgccgccag ctcgaggaac cggcggcgat cgaggccgga atcccagagg gcagcgtggc
     6601 gggggaactc gcgctcgatc cggtcgcgaa actcgggcga gcgggccagg tcggccaggc
     6661 tcgtccagtt gtcgcggccg tgcatcgagg tcggcgggcg gcggggggcg ggatgggctt
     6721 cggcggcggt catggcgggt atccgttcta acgatggcag gtggagcagt cgatgcgccg
     6781 ctggatgccg tattccctga ccagccgcgc gcccagggcc tgctggtcgg cgggcggtgt
     6841 gtaatccgga ttgaagacct cttcccgggg acgcacatat cgttccggtt tggcgtggca
     6901 attgaggcac cagcgcatgg agaggtcggc gaccttgcgg gtgaggggca tttcgccgac
     6961 cgggccatgg caggtggtgc agccgatccc ctggcgcaca tggacgctgt ggtcgaagta
     7021 gacaaaatcg ggcaggtcgt agacgcggat ccagcggagg ggcacgcccg cggcgaggct
     7081 gcggcggacg gggtcgagca tcggggcgtc gcgccacagt tgggaatggc agctcatgca
     7141 ggtgtgcgtc ggggggagcc cggcaaaggc cgagtccgcc acggaggcgt gacagtagcg
     7201 gcagtcgatg ccgatgcggc cgacgtggtg ctggtggctg aacgggacgg gttgagcggg
     7261 ggcatgattg acccgggtga ggtagtcgga gcgggtaaag gcggtccagg cccaggcgcc
     7321 ggcgccgagg aagacgagga gcgccgcgag gctccagcgg gcgaggacat tggcgaccgg
     7381 cggaaagagc tgcggcacgg cgcgccccct aggtggatgc tcccgcgcgc gagaggcggc
     7441 ggtgaaatct agacacttta ctccagacca gttgctgatg ctcccgcgcg cgagaggcgg
     7501 cggttcagat cataggccgc gctgatcagg ccgaggtgcg tgagggcctg ggggaagcag
     7561 cccagtagtt cgccgttggg cccgatttcg gccgaaaaca ggccgaggtg attggaataa
     7621 gtgaacattt tttcgagcat caggcgggcg cgatccagct cgcccatgcg cgagaggcac
     7681 tcggcgtacc agaaggagca gacgctgaag ctggcgagcg gctgatcgac ggcgtcgggg
     7741 ggcgcgccat ccggataccg gtggcgatag accagcgcgt cctcggcgag gtggcgttcg
     7801 atggcgcgca gcgtgccgat ccagcgcggg tcggtcgggc cgacgaactt gaccatcggc
     7861 atcatcaggc aggcggcgtc gagggcgggg gagtccttcg cctggatgaa gctttggcgc
     7921 tgggtgtccc agaagttctc catcagatcg ttgtagatgg cgtcgcggat ctcgcgccag
     7981 cggtcgagcg gggccggaaa ggagcgcttg agcgcgaggc gaatggcgcg gtcgaccgcg
     8041 acccagcaca tcaggcgcga gtaggcatac tcgtggggtt caccgggcag atcccagatc
     8101 ccccggtcgg ggtcccgcca gtgggcgcaa acgtattcga cgtggcgcgt caccttgcgc
     8161 caggtggcgt cggcgatcgg cgcgccgaat ttgttgtaga gatagatcga atcgaggagt
     8221 tcgccgtaga tgtcgatctg attcggggcg gggtcggcgt tgccgatgcg gacggggccg
     8281 gcgccgcggt agccgcgcag aggagtcagc tcgcgttcgg ccggctcgag gcggccgtgg
     8341 atgccgtaaa tcacgcggag cgacccgtcg ggcgcgattt cgtcgcagcg gtggctgagc
     8401 caatcgatga aggaggcggc ctcgtcggtg aaaccgagcc ggaggaaggc atagacggtg
     8461 aacgaggcgt cgcgaatcca ggtgaagcgg tagtcgaagt tgcgccggtg atcgctggtt
     8521 tcgggcaggc cgaaggtggg ggcggcgagc agcgcgccgg tcgggcggaa ggtgagcagt
     8581 ttgagggtca gcgccgagcg gttgaccatc tcgccccagc ggccgtggta gctggagcgg
     8641 ctgagccaga tacgccaata atcgatggtc tcctgaaagg cccggtcggc gtaatccgga
     8701 gcggaggagg gggattcgtc ctcgtcctgg agctcttcca ggatgacatc caccgactgg
     8761 ccggccttga gcgtgaagcg cgagagggcc cggccgcggc ggacggcgag ggggatcgtg
     8821 ctgcgcaggc ggacggtcgg caggttgggc gcatcgggga tgaagaggag cgtcgcgccg
     8881 tgtttttcga cgcggtgcgc ggcgcggccg tagtcgaagc gggggtcgaa gacgagctcg
     8941 aattcaactt caccgcggat cgtgcggacg cgccggatca ggtcatgcgc atgccccatc
     9001 tcctcgatcg gcatgaagtc gcacagttcg gcgacgccgt cggccgacat gaagcgggtg
     9061 agcaggatgc aggtgtcggg caggtacatc tgcttgcgct gggcggcggc gaagcggggc
     9121 gccaggcgga aggcgccgcc gcgcgcatga tcgaggagcg cggcgaacag cgtcggcgag
     9181 tcgaaggagg gaaaacacat gaagtcgatc gtgccatcca tggcgaccag cgccacggtg
     9241 ttcaagtcgc cgatgacacc gtggtcgccg atcggcgggg aatcgggcgt ggggcggcgc
     9301 gggctcacga gccgtccgcc ccatgcacga gaacgtgctt gataccggcg gggtggtcga
     9361 gcagcgggcg gcggaaatcc tcgagcgggt ggcgggccgt gatcaactgc tggaggacgc
     9421 cgggccagcg ccgggccatg cggcccagat cgtcgacggc ggcccgataa gcgtcgcggc
     9481 cggcattgac cgtgcccagc acgacctggt ttctgagcac gaggttctgc atgatcgccg
     9541 cggcgtcgca ctggatcggg tgtttgcgcc cgggcacgcc ggtgaagacg aagacgccgt
     9601 tggcgccgag ttcgcgcagg agcgcaaacg acacctcggc cgcgccggtg gcttcgtaga
     9661 ccagatcgat gttgcccacc gcgcgcgcca ggtcgggagg gtcggcgtcg gcggcggaga
     9721 ggtagcgcgc gccgatctgt tgggccagac gggccttggg accgccggcc ggctcgcggg
     9781 aatagaccgt cgtttcgaag ccggtgagca cgaggagcat ggcgccgagg agcccgacgg
     9841 gcccggcgcc gaggacgacg gcgcgatggt ggaagccctg gtcgctctgg ccgaggtcgc
     9901 cggcgaggcg cgccgccgcc gtcgcgcggt ccgatttgtg gcggctgggg ccggggagcc
     9961 gctcctgcac gtcccagacc tgggtcaggg ctttctcggc gatcgtgagg ggttcggtga
    10021 ggacggcgat gtggcgcagt tcgctgggga ccgggatgaa gaactgttcg ggatcgacga
    10081 cggtttcggc ggtgaagccg tgggcggcct tgatgccgcg ctcggtgaaa tcgccggtgt
    10141 aacagaaatc ggcgcgatgg gcacggcagg ggcggcagtg ggcgtgcggg cagggacgcc
    10201 gcaccatcgg gatgaccaga tcgccgggac ggagggcggc gacagcgggg ccgacgtcgg
    10261 cgaccaggcc gaggccttcg tggccgatga cgaggaacga gctgccgggg ggcggggcgc
    10321 catactggaa gctgacgatt tcgcggtcgg tgccgcagag gccgacttcg agcatgcgga
    10381 ccttgacgtc gtgccggccg cggcgcagcg gctcgtcgag ttccaccaga tcgatttgtc
    10441 tgctcctggg gaagacgcca atcgctcgca tggagggggg agtccgtttc caaatcaggg
    10501 gggcggcggg gtttgggggg gtcggatgag gcggcggggc cgtgggccgg tcccgccgcc
    10561 cgagccgccg ccgggtgcgc ggcgcattca ccgggggagc aggtcaggac gacgtgcgtc
    10621 cttccttctt gcgttgttta ttgaccgttc gagcggcgat ctctttcgcg cgcttggccg
    10681 aacggccccc cttcctctcg ctggacttca cgtgctcgta ttgacgctcg tcttttttgg
    10741 tccatgattt gggcattgtt ctgatccttt cggtggatac ggctgaagta agactggagc
    10801 aagattcaga ccggcggaag gggggacacg ggaatggcgg ggttgccgaa ttcgcccctt
    10861 cggggacggg gatgtttgcg atagcatccc caggttccgc tgcgcgccac caggggctat
    10921 ttatatccgg ccccggccgg ggcggcccat ggagacggcg ttttgcgaat ttgaaacaat
    10981 ggcggctgag agaaggacga aatgagttgg gcatgaaccg ggttcatgag aaagggacaa
    11041 attggggtag gcctttgggg gcgattacga agacgagggt cgattt
//
LOCUS       DAOPCO010000124        11048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5781_length_11048_cov_5.052761, whole genome shotgun sequence.
ACCESSION   DAOPCO010000124 DAOPCO010000000
VERSION     DAOPCO010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5781_length_11048_cov_5.052761"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="PLS90_09795"
     CDS             <1..1307
                     /locus_tag="PLS90_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02733.1"
                     /translation="FQGGSESWYAYNNPDELQFWHSKNHWDDPWLEWDTAPDVAAGGS
                     LEVQLDARPAFTATGRRQRIQLLISTAADLAAPTGVPAAQATLTVENVDNVAQAYFQA
                     SGVGGAPVALGIAATDIISMRMFITYDQATTSFKGYLKLNDGFIRQIGQPVVAARTTT
                     VAHFRQNVTSADPANPDPFDVFLQELRVYDGIVTTQGDPIPVGTLLREASYNAEPTGD
                     FFQPIGEKDADQLYMPDPTEHGYTGEGTFFVTKGPENNVYPNMSINTVTGVNEPAEWV
                     IDVEPRMTADGIGQHFNVKLGDGNVYMGNEEGRGEGWGFLFGELNDPFNPDPANVQLV
                     PTPITQVQKLRLIINYDADTQTWRFFWRVNDGPLREHPSSPLSAPRTATKVEFQYQVW
                     NSDNSNPSPFTIYHDHYAEYYGVVTTEADPLPPNAARHWAIY"
     gene            1389..2108
                     /locus_tag="PLS90_09800"
     CDS             1389..2108
                     /locus_tag="PLS90_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02734.1"
                     /translation="MQRTNTTANGPGRGFTLIELLIVVAIIAILAAIAVPNFLEAQVR
                     AKVSRVKADHRTLAVGLESYVIDFNNYPPNIYSYADQGWIQGGYDEPWNLNSWDHRRG
                     RTVAATLWRLTTPVAYLASIESMRSPFWAYENYWDWTLTPNGPTPSYYNVGSTYRFGA
                     CKGGSGGRPGRGMPTHDTRMDPNVFQEYVGAGMIKPNHHWYIHDPGPDRRFTLDEGSQ
                     EHHPYDPTNGTVSDGEIVTTG"
     gene            2404..6129
                     /locus_tag="PLS90_09805"
     CDS             2404..6129
                     /locus_tag="PLS90_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02735.1"
                     /translation="MLKHPTMWATAALIGLTSLCGSPRPRAALGEREIPPPAAHAAPR
                     PLLADFNAGLEGRGVRREGRHADDQRVTTTWDQAVAFEGEGSLRIDYRLTPGGQSVAV
                     ETVRLEGRALPDDLYGFEFQARTDAPDAVELLIDLRERSGEIWQQATVFPARPQPGEW
                     MRIPVVLDMLRLVTWTRENGRFDLDQVEGLDLVLSGARGADGPRSGSLWIDAFGPLDP
                     WRTIGGTDHDGFHLLDLSGVFNSDAVAWKSNPLDGDFHTGEWQQRTLPAEHWPDAPVA
                     TYHGVPFAMPSVADGLNNHLRSQGQCLTGFAAGPYSAAYFLASGKFGDQAGDVTLYYA
                     DGTTTRAALDISDWSVGPTRGDQVAIRFPFTYHEHRTEPRMPMIYLQSIRLDPTRPLV
                     GLRLPRRDYLKVFSITLARRDPPPAAGALHDRPLEEIDPRYRVVIPEELSFAEPARSA
                     GVYGGYLARRNEEPVIDVPTLNGGLAFSIEGVQFPRHTLQWPRALLDNSDYPLGQIQI
                     DARQAGHPILELVSSTCMTYTAPLEGHDGHPFKVYLSRVSPAALYEIGLEHYRWHEPG
                     GGPTHVAYADGDGVTVAPLKEGAALPLPAEPWLLLWHADRKPDAFDAPMLLVLERKPE
                     RIEVARRMGSLGLNFRHAGTAGHVAVMPLHGIERIAADVTAGWAAGLPADVAQRCRAW
                     AARLQRYPLEVTTTPAPDEAAGRITFTEHFRYLDLATQWDVTPRSIAPVPPVAALARA
                     HGYPVEWGAGLRPLDYATLWGPLVAAEGDSASWSIPLPDYLERTLIPTNVTGDPFIEQ
                     MKQDLAGRLRNIPRRINSDNSGDSGAMKLYAPAHLIIGENAAIADYCRRVARNILNPD
                     NIKVEKELQTGQFFLMDDRFWCADAAYDKEWAIGHMLQGLWQAAYHLDDTALLRENWD
                     TIRGLYRYYQIIFDWPTASTYTMVTGQGANSDGIRFAWEGMLGMARMARMVGDEPTWR
                     DVAARSALQMMNLYASWFAPQWAVAHDYTVAAGRRIPVSEAETGFAPDGWAETTGVGL
                     AHDGDFFQVTHAFFMYHPDQLLYLHDYGLDEARLRPWLMERVPALHPNWDDGNVRSDG
                     WYYGSSHSVAHLVVRSLLLHQPAEEVHAHLRRMLDDTRVLKEWYSPEGAASFALMAMV
                     TGAAPLAAAPVGHYYVTRHDYDAAGGRQVLEMRRTRPGATELRVRCWGRVPRAVEVNG
                     EPRQIDYDPASDYLRVPLTDDDGETARVVVQHGEGEP"
     gene            6126..7802
                     /locus_tag="PLS90_09810"
     CDS             6126..7802
                     /locus_tag="PLS90_09810"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HPK02736.1"
                     /translation="MMPVDLGTTVAEVAWVAPSPRLEWGFELHDVDGNPFEAEIDGWI
                     ERPDGERFAAPAFFDGDAVWRIRFTPEQSGVYRLAGVTRRTSSGRHELEAALQTPAAY
                     GVRQAPAPGFVRRLASDPSRFGLDGTRPWYPFGMNVAWHAEDQFPLCFARMSEAGMNW
                     SRVWMVHWAGLNLDWFMRGDKLGQLDLEVARRWDRIIESAEQHNIRFQLVLQHHGQVS
                     TFVNSNWHDHPWSRARGGFLAHPAEFFTHPRALELTRRKYRYIVARWGYSPAVMAWEL
                     FNEVEYTNGYLGAKVKRWIDHFVDLGQHFAGADGHAVAEWHAEMAALLRSVDVHRHLV
                     TTSAAEPEHPLWESMDYLQGHCYRHDMLGAVSAIPDGADPERPFFWGEIGDHNMAYPD
                     KADGRYTRAMLWAGLMSGAAGAAQIWAWDRVEALDLYRDFAAAAAFIRAGDFAGRRLT
                     RQPVQVEPAPGADATAGPFAMAVADDERVAGWAYHGLGVHHPGEEAVRGRLTLRGRRA
                     GRWRVDWLSTGEPAVLDGCELEVGPEGALVLETPDIRRDVAFVARWLEER"
     assembly_gap    8006..8105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8183..9094
                     /locus_tag="PLS90_09815"
     CDS             8183..9094
                     /locus_tag="PLS90_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK02737.1"
                     /translation="MTTQQTQATRRGALLRTGTFERFFDQSHGEAEPWYINDHCVARD
                     RNGLWHLFGITHAEPLNPLDEKNLAHATSPSLAQRPWDKHPFALTADPAYDEHHLWAP
                     FVVTHEDTYYMYVCVGARNNHRYKIHLFTSQDMFNWKRHAENPMVVDGFDARDPCILR
                     LDGEWLMYYTATIEPAGGNHIVACRRSKDLVHWGERSVVFVDEETGTFGGSTESPCVV
                     RRGDVFYLFICNNDRRSGYDATDVFASRNPLHWTMEDRVATLNAHAAEVIRDVDGKWY
                     ITHCGWGRGGAYIAPLYWNDGLADEAG"
     gene            9081..9998
                     /locus_tag="PLS90_09820"
     CDS             9081..9998
                     /locus_tag="PLS90_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019928772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPK02738.1"
                     /translation="MKPVEPLPAAVRSGAPPLSVGPFRRIYDPCAGRREPWCINDHCF
                     ARDPAGRWHLFGITHLEPFNWSLDPARNLAHATADHLTQSPWRREPDALTADAAHGEI
                     HLWAPHIVRHEGRYQMFVCVGNDDHTRYRIHLLASTDLWRWERHPANPIVIDGFDARD
                     PCVIRLGEQWVMYYTATEPAEGGRHIVACRTSGDLVRWGERRIVYTDEECGTFGGPTE
                     SPFVVQRGDWFYLFICNNDRRAGYDATDVLRSRDPFHWNFSDRVATLPAHAAEVVRDE
                     RGDWYLSHCGWFRGGVYLAPLTWHDGVAD"
     gene            10075..10371
                     /locus_tag="PLS90_09825"
     CDS             10075..10371
                     /locus_tag="PLS90_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02739.1"
                     /translation="MFRRISGRADFALCQQLHYLQMATQKLARAYLSSPRGDPPPRTH
                     PALSRFLRLRKGRPELRQRMGYGGNYVACCAYIDSLMNVATRVESRRPLERASG"
     gene            complement(10510..10938)
                     /gene="lspA"
                     /locus_tag="PLS90_09830"
     CDS             complement(10510..10938)
                     /gene="lspA"
                     /locus_tag="PLS90_09830"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPK02740.1"
                     /translation="MAVAFLRGRPPLELIPGYLRLWYAENTGAAFSVLEGHTEILAGF
                     SLIVSVVIFVWGWRLSPREQGLRVALGLILGGALGNLIDRVYLRYVIDFISAHWHYKY
                     VWPTFNIADSAVCVGMFLLIVASFRIPSAPAKSADSPGST"
BASE COUNT         1843 a         3701 c         3669 g         1735 t
ORIGIN      
        1 cgttccaggg cggcagtgag tcctggtacg cctacaacaa ccccgatgag ctgcaattct
       61 ggcactctaa gaaccactgg gacgatccgt ggctggagtg ggataccgct ccggacgtcg
      121 cggccggcgg ttcgctcgaa gtgcagctcg acgcccgccc ggccttcacg gccaccggcc
      181 gccgccagcg catccagttg ctgatttcga ctgccgccga tttggccgcc ccgaccggcg
      241 ttccggcggc ccaggcgacg ctgacggtcg agaacgtcga taatgtcgcg caggcctact
      301 tccaggccag cggcgtcggc ggcgcccccg tcgccctggg catcgcggcg accgatatca
      361 tctcgatgcg gatgttcatc acctacgacc aggcgacgac gagcttcaag ggttacctga
      421 agctcaacga cggcttcatc cgccagatcg gccagccggt cgtggcggcg cgcaccacca
      481 ccgtggccca ctttcgccag aacgtgacgt ccgccgaccc ggccaacccc gacccgtttg
      541 atgtcttcct gcaggagctg cgggtctacg acggcatcgt cacgacccag ggcgacccga
      601 tccctgtcgg cacgttgctg cgcgaggcaa gctacaacgc cgagcccact ggcgatttct
      661 tccagccgat cggcgagaag gatgccgacc aactctacat gcccgatccg accgaacacg
      721 gctacaccgg cgagggaacg ttcttcgtga cgaagggccc ggagaacaat gtctacccga
      781 atatgtcgat taacacagtg accggtgtga atgaaccggc cgaatgggtg atcgacgtgg
      841 agccgcgcat gaccgccgac ggcatcggcc agcacttcaa cgtaaaactg ggcgacggca
      901 acgtctacat gggcaatgag gagggccgcg gcgaaggctg gggcttcctg ttcggcgagc
      961 tgaacgaccc cttcaacccg gatccggcga acgttcagct ggtgccgact ccgatcacgc
     1021 aggtgcagaa attgcggctg atcatcaact acgacgccga cacgcagacg tggcgcttct
     1081 tctggcgcgt caacgacggg ccgctgcgcg agcacccgag ctcgccgctc tcggccccgc
     1141 gcacggcgac caaagtggaa ttccaatacc aggtctggaa cagcgacaac tccaacccgt
     1201 cgccgttcac tatctatcac gaccactatg ccgaatatta cggggtcgtg acgaccgagg
     1261 cggacccgtt gccgcccaat gccgcccgtc actgggcgat ctactgaccg tgaccgccgg
     1321 agaccccgct catccgtcgc gggcggggtc tcctccccct ctttgaatgt ttgtcgcgag
     1381 gtaaggacat gcaacgcacc aacaccaccg cgaacggccc gggccgggga ttcacgttga
     1441 tcgagctgct gatcgtcgtc gcgatcatcg cgattctggc ggcgatcgcc gtgcccaact
     1501 tcctggaagc ccaggtgcgc gcgaaggtct cgcgcgtcaa ggccgatcac cggacgctgg
     1561 ccgtggggct ggaaagctac gtaattgact tcaacaacta cccccccaat atctacagct
     1621 atgccgacca gggatggatt caggggggct atgacgagcc ctggaacctc aattcctggg
     1681 atcaccgccg ggggcggacc gtcgcggcga ccctctggcg cctgacgacg ccggtcgcct
     1741 acctggcctc gatcgaatcg atgcgctcgc ccttctgggc ctacgagaac tactgggact
     1801 ggacgctgac gccgaacggc ccgacgccga gctactataa cgtcggctcg acgtatcgct
     1861 tcggcgcctg caaggggggc agcggcggcc gccctggccg cggcatgccg acccatgaca
     1921 cccgcatgga ccccaacgtg ttccaggaat acgtcggcgc ggggatgatc aagcccaatc
     1981 accactggta tatccacgac ccgggcccgg atcgccgctt cacgctcgac gagggttccc
     2041 aggaacacca tccgtatgac ccgaccaatg gaacggtgag cgacggcgaa atcgtgacca
     2101 ccggctgagc cgcggccggc cccggttacc gttcaaatcc ctgccttgcg gcccttggct
     2161 cccgcgccgg cgtctggatg cgatccgggc gccggccgcg ggaggaaacg gggccgaact
     2221 ccccggaccg ctcccggccg gcgtgccgcg ccggtcgctc cagcccgcgc gcccccagat
     2281 gccctgctcc cggccggctc gaataagggg ccccgttcga ccggcatcgg ctcgcttcgc
     2341 ggcctggcag gtccgcggcc tggcaagtcc catgaaacat cccaaggacg cccgatcccc
     2401 gtcatgttga agcatccgac aatgtgggcg accgccgcgc tgatcgggtt gacctccctc
     2461 tgcggaagcc cgcggccgcg ggcggcgctg ggggagcgcg agatcccgcc ccccgccgcg
     2521 catgcagcgc cgcggccgtt gctggccgac ttcaacgcgg gtctggaggg acgcggcgtc
     2581 cgccgcgagg gccgccacgc cgacgaccaa cgcgtgacga cgacgtggga ccaggcggtc
     2641 gcgttcgagg gtgaagggtc cctgcggatc gattaccggc tgacgccggg gggccagtca
     2701 gtggccgtcg aaacggtccg gctggagggg cgggcactgc ctgacgatct gtatggcttt
     2761 gaattccagg cgcgcaccga tgcgcccgac gcggtcgaac tgctgatcga cctgcgcgag
     2821 cgctcgggcg aaatctggca gcaggccacc gttttccccg ccaggcccca accgggcgag
     2881 tggatgcgca tcccggtcgt gctcgacatg ctgcggctgg tcacctggac gcgcgagaac
     2941 ggccggttcg acctggatca ggtcgagggc ctggacctgg tgttgagcgg cgcgcggggc
     3001 gccgatggtc cgcggtccgg ttcgctgtgg atcgatgcct tcggcccgct cgacccgtgg
     3061 cgcacgatcg gcgggaccga ccatgacggc tttcatctgc tcgacctgag cggcgtcttc
     3121 aattccgacg ccgtggcctg gaagtcgaat ccgcttgacg gcgacttcca caccggcgag
     3181 tggcagcagc ggacattgcc ggccgaacac tggcccgacg cgccggtcgc gacctaccac
     3241 ggcgtgccgt tcgccatgcc gagcgtcgcc gacgggctga acaaccatct gcgcagccag
     3301 ggacagtgcc tgacggggtt cgccgccggc ccctacagcg cggcctattt tctcgcctcg
     3361 ggcaagttcg gcgaccaggc cggcgacgtg acgctctatt acgccgacgg cacgacgacg
     3421 cgcgccgcgc tcgatatcag cgactggtcg gtcgggccga cgcgcggcga ccaggtcgcg
     3481 atccggttcc cgttcactta tcacgaacat cgcaccgagc cgcgaatgcc gatgatctac
     3541 ctccagtcga tccggttgga tccgacccgg ccgctggtcg ggctgcggct gccccggcgc
     3601 gattacctta aggtgttttc cattacgctg gcgcggcggg atccgccgcc cgcggccggc
     3661 gcgctgcacg accggccgct ggaggaaatc gacccgcgtt accgggtcgt catccccgag
     3721 gagctctcat tcgccgagcc ggcgcggtcg gcgggcgtct acgggggcta cctggcgcgg
     3781 cgcaacgagg agccggtgat cgacgtcccg acactcaacg gcggcctggc gttcagcatc
     3841 gaaggggtcc agtttccccg ccacacgctg cagtggccgc gcgcgctgct cgataacagc
     3901 gattaccccc tgggccagat ccagatcgac gcgcgccagg cggggcatcc gatcctggaa
     3961 ctcgtcagct ccacctgcat gacctacacg gccccgctcg agggccacga cggccatccg
     4021 ttcaaggttt acctgtcgcg ggtgagcccg gcggcgctct acgaaatcgg cttggagcat
     4081 taccgctggc atgagccggg cggcggtccg acgcatgtgg cctatgcgga cggcgacggg
     4141 gtgaccgtcg cgcccctgaa ggagggcgcc gcgctgcccc ttcccgccga gccgtggctg
     4201 ctgctgtggc atgcggatcg gaagcccgat gcattcgatg cgccgatgct gctggtgctg
     4261 gagcgcaagc cggagcggat cgaggtcgcg cgccggatgg gatcgctcgg cctgaatttc
     4321 cgccacgcgg gcacggccgg ccatgtcgcg gtgatgccgc tccacgggat cgagcggatc
     4381 gccgccgacg tgacggccgg ctgggccgcg ggattgcccg cggacgtggc gcaacgctgt
     4441 cgcgcgtggg ccgcccgcct gcaacgctat ccgctggaag tgacgaccac tcccgcgccg
     4501 gatgaggcgg ccgggcgcat cacattcacc gaacacttcc gctacctgga cctggcgacc
     4561 cagtgggacg tgacgccgcg gtcgattgcg cccgtcccgc cggtcgccgc cctcgcccgc
     4621 gcgcacggct acccggtgga atggggtgcg ggcctgcgtc cgctggacta cgccacgttg
     4681 tggggaccgc tcgtcgccgc cgagggagat tcggcctcct ggagcatccc gctgccggat
     4741 tatctggagc ggacgctgat cccgaccaac gtgacgggcg atcccttcat cgaacaaatg
     4801 aagcaggacc tggcgggccg gctgcggaac atcccgcggc ggatcaactc ggacaattcg
     4861 ggcgacagcg gcgcgatgaa gctctatgcc ccggcgcatc tgatcatcgg cgagaacgcc
     4921 gcgatcgccg actactgccg ccgcgtggcc cgcaacatcc tgaatcccga caacatcaag
     4981 gtcgaaaagg aactccagac cggccagttc ttcctgatgg acgaccggtt ctggtgcgcc
     5041 gacgcggcct acgacaagga atgggcgatc ggccacatgc tccaggggtt gtggcaggcg
     5101 gcctatcacc tcgacgacac cgccctgctg cgcgagaact gggacacgat ccgcggactg
     5161 tatcgctatt accagatcat tttcgactgg ccgacggcga gcacctatac gatggtgacg
     5221 ggccaggggg ccaactccga cggcatccgt ttcgcctggg agggcatgct cgggatggcg
     5281 cggatggcgc ggatggtggg cgacgagccg acgtggcgcg acgtggcggc gcgctcggcc
     5341 ctccagatga tgaacctcta cgccagctgg ttcgcgccgc aatgggccgt cgcgcacgat
     5401 tacaccgtcg cggccggccg ccgcattccg gtgagcgagg cggaaacggg cttcgccccc
     5461 gacggctggg cggaaacaac cggcgttggc ctcgcccacg atggcgattt ctttcaggtc
     5521 acccacgcct tcttcatgta ccatcccgat cagttgctct acctgcatga ctacgggctg
     5581 gacgaggcgc gcctgcggcc gtggctgatg gagcgcgtgc ccgcgctgca tccgaactgg
     5641 gacgacggca acgtccggag cgacgggtgg tactacggca gttcgcactc ggtggcgcac
     5701 ctggtcgtcc gctcgctgct gctgcatcag ccggccgagg aggttcacgc gcacctgcgc
     5761 cggatgctgg acgacacccg cgtgctcaag gagtggtatt cgccggaggg agcggccagc
     5821 ttcgccctga tggcgatggt gaccggcgcg gcgccgctcg cggctgcgcc ggtggggcac
     5881 tattacgtca cgcgccacga ctacgacgcc gccggcggac ggcaggtcct ggagatgcgc
     5941 cgcacccggc cgggcgcgac cgaattgcgc gtgcgctgct ggggccgcgt cccgcgcgcc
     6001 gtcgaagtca acggcgagcc gcgccagatc gactatgacc ccgcctcgga ttatctgcgc
     6061 gtgccgctga ccgacgacga tggcgagacg gcgcgcgtcg tcgtccagca cggggagggc
     6121 gagccatgat gccggtggac ctggggacca cggtcgccga ggtggcgtgg gtcgcgccga
     6181 gcccgcggct ggagtgggga ttcgagctgc atgacgtgga cgggaacccg ttcgaagccg
     6241 aaatcgatgg ctggatcgag cggcccgacg gcgagcggtt cgcggcgccg gccttcttcg
     6301 acggcgacgc cgtctggcgg atccgcttca cgccggagca atccggcgtc tatcggctcg
     6361 ccggcgtgac gcgccgcacc tcatccggcc ggcacgaact cgaggcggcg ctgcagacgc
     6421 cggcggccta cggcgtgcgg caggcgccgg cccccgggtt cgtccggcgg ctggcaagcg
     6481 atcccagccg cttcggcctg gatgggacgc gaccgtggta tccgttcggg atgaacgtcg
     6541 cctggcacgc cgaggaccag tttccgctct gcttcgcgcg catgagcgag gccgggatga
     6601 actggtcgcg cgtctggatg gtgcattggg ccgggctgaa cctggactgg ttcatgcggg
     6661 gcgacaagct gggccagctc gatctggagg tcgcccgccg ctgggatcgg atcatcgaat
     6721 cggccgagca gcataacatc cgcttccagc tcgtcctcca gcaccacggc caggtcagca
     6781 cgttcgtcaa ctccaactgg cacgatcatc cgtggtcgcg ggcgcggggc ggattcctgg
     6841 cccatccggc cgagttcttc acgcacccgc gcgcgctcga gctgacacgg cgcaaatacc
     6901 gctacatcgt cgcccgctgg ggctattccc ccgccgtgat ggcgtgggaa ctgttcaatg
     6961 aggtggagta tacgaacggc tacctcgggg caaaggtcaa gcgctggatc gatcactttg
     7021 tcgacctggg ccagcacttc gctggcgccg acggccacgc cgtcgccgaa tggcacgccg
     7081 agatggccgc cctgctgcgc tcggtcgatg ttcaccgcca tctggtgacg acgagcgccg
     7141 ccgaaccgga acatccgctg tgggagtcga tggattacct gcaggggcat tgttaccgcc
     7201 acgacatgct gggcgccgtg agcgccatcc cggacggcgc cgatccggaa cggccgttct
     7261 tctggggcga gatcggcgac cacaacatgg cctaccccga caaggccgac ggcagatata
     7321 cgcgggcgat gctgtgggcg gggctgatgt cgggcgcggc cggcgcggcg cagatctggg
     7381 cctgggaccg cgtcgaggcg ctggatctgt atcgggattt cgcggcggcg gcggcgttca
     7441 ttcgcgcggg agatttcgcc ggacgccggc tgacgcgcca gccggtgcag gtcgagccgg
     7501 cgccgggagc cgacgcgacg gccgggccgt ttgccatggc cgtcgccgac gatgagcggg
     7561 tggccggctg ggcctaccac ggcctcggcg tgcatcatcc gggtgaggag gcggttcgcg
     7621 gtcggctgac cctccgcggc cggcgcgcag ggcgttggcg cgtcgactgg ctcagcaccg
     7681 gcgagccggc ggtactggac gggtgcgaac tcgaagttgg accggaaggc gcgctggtcc
     7741 tggaaacccc cgatatccgc cgcgacgtgg cgttcgtcgc ccgctggctg gaggaaaggt
     7801 aatggacgca agcatcggga tcgggagcgc gatcgcttgc ggacgaccgg aagagagcgc
     7861 agggtgacgg ccgcgggccg gaaccctgcg tcgccctccc gacgcatcaa accccagaaa
     7921 ggggtggccg gagatggtcc gaattcgggg aatggcaagg aataaaatgg ggggtgaacg
     7981 acccaggcct tgccggatcc ttcagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnncaggg acggcagaac accttgaatc gatcacgaca tgggacagac gaagctcaga
     8161 cggtgaggag atcatgcatc acgtgacgac ccagcaaacg caagcaacca ggcgcggggc
     8221 gctgttgcga accggcacgt tcgaacggtt cttcgaccag agtcacggcg aggccgagcc
     8281 ctggtacatc aatgatcact gcgtcgcgcg cgaccgcaac ggcctgtggc acctgttcgg
     8341 gatcacgcac gccgagccgc tcaacccgct cgacgagaag aacctggccc acgcgacctc
     8401 gcccagcctg gcccagcgcc cgtgggacaa gcaccccttc gcgctgacgg ccgaccctgc
     8461 ttacgacgag catcacctgt gggcgccctt cgtcgtgacg catgaagaca cctactacat
     8521 gtatgtgtgc gttggggcgc gcaacaacca ccgctacaag atccacctgt tcacgtcgca
     8581 ggacatgttc aactggaagc gccacgccga aaatcccatg gttgtggatg gatttgatgc
     8641 gcgcgaccca tgcatcctgc gcctggatgg cgaatggctg atgtattaca cggccacgat
     8701 cgagccggcg ggcggcaatc atatcgtcgc ctgccggcgg agcaaggacc tcgtccattg
     8761 gggcgagcgg agcgtcgtct tcgtggatga ggagaccggc acgttcggcg gctcaaccga
     8821 atcgccctgt gtcgtgcgcc gcggcgacgt cttctatctg ttcatctgca acaacgaccg
     8881 ccggtccggc tacgatgcga ccgatgtctt tgccagccgc aatccgcttc attggacgat
     8941 ggaggatcgc gtggcgacgc tcaatgcgca cgcggccgag gtgatccgcg acgtcgacgg
     9001 caaatggtat atcacccatt gcggttgggg ccgcggcggc gcctacatcg ccccgctgta
     9061 ctggaacgac gggctggctg atgaagccgg ttgagccgct tccagctgct gttcgctccg
     9121 gcgcgccgcc gctgagcgtt ggacccttcc ggcgcatcta cgacccgtgc gccggccggc
     9181 gcgagccgtg gtgcatcaac gatcactgct tcgcgcgcga tccggccggg cgctggcacc
     9241 tgttcggcat cacgcatctc gagccgttca actggtccct cgacccggcg cggaatctgg
     9301 cgcacgcgac ggccgaccac ctgacgcaat cgccgtggcg gcgcgaaccg gacgcgctga
     9361 ccgccgacgc ggcccatggg gaaattcacc tgtgggcccc gcatatcgtg cggcatgagg
     9421 ggcggtacca gatgttcgtc tgcgtcggca acgacgatca cactcgctac cggattcacc
     9481 tgcttgcctc gaccgacctc tggcgctggg agcgccaccc ggccaacccg atcgtgatcg
     9541 acggcttcga cgcgcgcgac ccgtgcgtga tccggctggg cgaacagtgg gtgatgtatt
     9601 acacggcgac cgaaccggcc gagggggggc ggcacatcgt cgcctgccgc acgagcggcg
     9661 acctggtccg ctggggcgag cgccgcatcg tctacaccga cgaggagtgc ggcacgttcg
     9721 gcggaccgac cgaatcgccc ttcgtcgtcc agcgcggcga ttggttctac ctgttcatct
     9781 gcaacaatga ccggcgcgcc ggctacgacg ccaccgacgt gctgcgcagc cgcgacccat
     9841 ttcattggaa tttcagcgat cgcgtggcga cgctcccggc gcatgccgcc gaagtcgtcc
     9901 gcgacgagcg gggcgattgg tatctgagcc actgcggctg gttccgcggc ggagtctacc
     9961 tggcgccgct gacatggcac gacggcgtcg cggattaacc ccgcctgttg acgggagcgg
    10021 ccgaccgggg catcgcgcag tgctttcttt cggcaagcac tgaacggtta tggcatgttt
    10081 cggcggatca gcgggcgagc cgatttcgcg ctttgccagc aactgcatta tctccagatg
    10141 gccacccaga agctggccag ggcgtatctt tcctcgccca ggggcgatcc cccgcctcgc
    10201 acgcatcctg ccctcagccg attcctgcgt ctgcgcaaag ggcgtccgga attgcgtcag
    10261 cggatgggtt atggcggcaa ctatgtcgcc tgctgcgcct acatcgacag tctgatgaat
    10321 gtcgcgaccc gcgtggaaag taggcgcccg ttggaacggg cgagcgggtg aaccccgaat
    10381 acccctggct cgatcgcacg gggaggataa tctgtcccgt cgattaccct tttcctgaat
    10441 tcagagccca cgatctggcg aaattccaac ggctgattgc ctgtttattc cgggcttttc
    10501 cctgatcgtt caggtggatc ccgggctgtc cgccgatttt gccggcgcgg acgggatccg
    10561 gaagctggcg acgatcagca ggaacatgcc gacgcagacg gccgaatccg cgatgttgaa
    10621 tgtcggccag acatatttgt aatgccagtg agccgagata aaatcgatga cgtaacgcag
    10681 gtaaacacgg tcgatcagat tgcccagcgc gccgcccagg atcaggccca gcgcgacgcg
    10741 cagaccctgt tcgcgcggcg agagccgcca gccccagaca aaaatcacca cgctgacaat
    10801 cagcgagaag ccggccagga tctcggtgtg accttccagc accgagaacg cggcgccggt
    10861 gttttcggca taccacaggc gcaggtagcc cgggatgagc tccagcggcg gtctgccgcg
    10921 cagaaacgca accgccagcc atttggtcag ttgatcgagc aggatgacga ccgcggccag
    10981 ggcagtgaac gcgacgcggc cgaccggcca ggcggtcgta gcagctcagc cgctccgcgt
    11041 cgcccggt
//
LOCUS       DAOPCO010000125        11039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5783_length_11039_cov_5.833758, whole genome shotgun sequence.
ACCESSION   DAOPCO010000125 DAOPCO010000000
VERSION     DAOPCO010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5783_length_11039_cov_5.833758"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PLS90_09835"
     CDS             <1..1334
                     /locus_tag="PLS90_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK02741.1"
                     /translation="TASPEETAPEPQAGAAEAPAAAASAAPAHDGFFTKDVIRHNMMF
                     LIMLEAILMTGSADFQIGLQPLLEYINAPYKLIGFIQGLSWTAILGCIATPYITRHFP
                     YKKWYYITVTSLWALSLAVLGIALVTSPVWNPSLFALTIVTLICVVFFNMTMGFTNTP
                     HNEFIASCIPPDHRGRLFGISTALYGLVSLGVIAISRYLLEVIEHPVSFGFIFIMTCV
                     IWLIGFGFASFAREMRTPVEKSPKPWTREMFRAFFLDKAYIRFVIFCALTHLTFMPLF
                     VLIPNYGLREMNMPATAAADIAFISQIVVIVTSTPSGWLIDKFGPKRMLPISVGLIGC
                     AYMFLFIVFTPLGIYSSMGLMKLALAVFYPCLTILSVSIPKPEDRAGHFSTFSIVGML
                     YFAAGPIIGGFVSDWIEPRTVVLIGSLLAFAFIPLTIVLLRVLATHLRDYY"
     gene            1888..2913
                     /locus_tag="PLS90_09840"
     CDS             1888..2913
                     /locus_tag="PLS90_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK02742.1"
                     /translation="MPHLPASIDTPEQLEDCLSLPYEETVQALRGLDGDIMVLGASGK
                     MGPTVARMAQRAVRQAGQDRKVYAVSRNPSRELAAAGIHTIACDLLDPDAVKELPRAE
                     NIIYMVGRKFGSKGAEHLTWATNVLAAGYAAAAFPDARFAAFSTGCVYPVMHIGSGGA
                     TEDEPPAPIGEYAQSCLGRERAFDYYSETHGLKVVHLRLNYSVELRYGVLVDIAQKVW
                     EQQPINLTTGYANVIWQGDACNQALQALALADSPARVLNLTGPETISIRRVALEFGKL
                     MDREPVFVGEENGFGYLSNAMRANRLFGNPRVPVGQVILWIAHWFQNQGVLLGKPTQF
                     ETQDGQY"
     gene            2959..4029
                     /locus_tag="PLS90_09845"
     CDS             2959..4029
                     /locus_tag="PLS90_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02743.1"
                     /translation="MPAARTLRIGIVGLGHLHPRLYMPHFQALPGVEVTAVAEPDQRL
                     REAFAADFGLRAYASLNDLLKHESLEIAAIFLPHAECAEAAARCAARGIHLMVEKPMA
                     SDVKGARRIVQAARKAGVQLTTGYCWRFHPVARRIKSLIAEGAIGRVISAEGRCAAGR
                     VTRYIEGHSDWMLEKARSGGGPIFNLGVHWIDLIRWMLADEVTEATGLNVHVNRRYDI
                     EDQSYAHLRFAGGAVLALDISYTVPDAYPHGRDLYLAIRGTDGVLSWTPGFEGETETL
                     FLCSDAPGFAGAPNQQISFALEPTPGYAGFMGRDYIARFVDAVRGTGEPPVSGEDAVA
                     VLNIVRAIYESDERKQRVKVRN"
     gene            4043..>4597
                     /locus_tag="PLS90_09850"
     CDS             4043..>4597
                     /locus_tag="PLS90_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02744.1"
                     /translation="MKAVVTDYIESDLEWEQAQLAPHGITLTAHQLKFRPADEVYEAV
                     KDADAIVVNMVKMDDALLRRLEKCKLLIRHGIGYDNVDVAACTRYGIQFAYQPDYCQI
                     DVAEHAVALILSLARRIPFSRATLENSSRTGQWDFSELFPIYRMEGKTLGIVGLGRIG
                     RRVARKLSGFGLKMLAATRRPMRPS"
     assembly_gap    4598..4607
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4608..5079
                     /locus_tag="PLS90_09855"
     CDS             <4608..5079
                     /locus_tag="PLS90_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02745.1"
                     /translation="KMLAADPYVDRAVAEDLGVRLVTLDELLGEADYITLHTSLTDET
                     RRLINADALGRMKPTALLVNTSRGPIVDQEALADALAEGRIAGAAIDVFDVEPPPIDY
                     RLFQSDRAILTPHIGWASEESAWEIRRRIVDDLISHAQGRPARCVINPEVLAAR"
     gene            5098..6435
                     /locus_tag="PLS90_09860"
     CDS             5098..6435
                     /locus_tag="PLS90_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPK02746.1"
                     /translation="MTITRVAGPLPGPRGRELLEQWHQYEADVVGFQAPVVWESARGC
                     VVRDVDGNLYIDWTSGVLVTNVGHCHPHLVRMINEAAQRLLNNYECANVERIEAARRL
                     VQALPEHLDRCFFLSTGSEAIEAAGRLLKRRTGKFEILTFEGGFHGRTTAAAAFGGMA
                     GPKRGYGPTLPGVIRVAFPNPYRDPFGWCDDGPEFPRYFDYLDQVVMANSTGSLAGVL
                     VEPYQGAAGFIFPPAGWLKRLERWARDRKLLFALDEVQSSYGRTGRMWALEHEDLRPD
                     IVTIGKGIGSGFPVSAVAATHDVFACLSKGEMSSTMGGNPVSSAAVIAVLDIMEREDL
                     VARSASMGEYMKQRLETMKAASPHLGDVRGMGLVLGLELVKDRESKQPAPELTRPLML
                     ACAERGLLVGSVGVHGNVIRVAPPLVISREEADESLNIMEQALRSLTPEHAAV"
     gene            6432..7673
                     /locus_tag="PLS90_09865"
     CDS             6432..7673
                     /locus_tag="PLS90_09865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK02747.1"
                     /translation="MIAAQAIHAAVAENLPEAVDHLCRLMAFPSLSGQEHEAMLHLEQ
                     VFSDLGLTVERVPMRPDLLRDPDYSSPIPGLAMEGRFNLRVVCPGRSARKIILNAHVD
                     VVPLAEGMAWGSRREGETIFGRGACDDKGPLITLWLVAATLTRAGVEPPCEIVLHLVN
                     EEENGGNGSLQMIRHGERADACIVMEPTEGRVFSSIRGAVWFRIDFRGQAGHSGQPGV
                     TRSALTMAREAMTALEDYHRRLLAESAGLPLFEAYPNPMPLTFGRLEAGNWPAAAPQH
                     ARLEGVLGFLPNRTRDQVCAELRAALLDAGQLKADDFELGFTYRHDCSMLDPEHFLAR
                     DLVQAARQAGYDSRIDAMTASCDAWFYNNQLSIPTVVFGPGSLRWAHTREEQIAIPEI
                     ARAAEAILLSILNSTGLQDDA"
     gene            7670..8479
                     /locus_tag="PLS90_09870"
     CDS             7670..8479
                     /locus_tag="PLS90_09870"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPK02748.1"
                     /translation="MKIVRFETGEGRIHLGRQRDDGAVERLDGELFDGLAPTGEVRQP
                     RRILAPLIPTTVYCIGANYRRHCDECNVAYPEQPIVFMKGANALVDPGQPIVMPSVCD
                     GDEVDYEGELAVVIGKRCKNVSRERALDYVLGYTCANDVSARNWQKNRGGGQWCRGKG
                     FDSFLPLGPALVTGDEIPDPAELTLRTWVNGDLRQESPTNDMIFDIPELIAFLSSGTT
                     LLPGTVILTGTPHGVGLGFDPPRMLRPGDEVRIEIDRIGALVNPVAAEAQA"
     gene            8518..9606
                     /locus_tag="PLS90_09875"
     CDS             8518..9606
                     /locus_tag="PLS90_09875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02749.1"
                     /translation="MAANTGFGILGAGLISPLHARAIHDAEGADLIAVCDMDLERARK
                     LAEPYGARVYDSLEAMLADPAIGVVCVVTPNHLHCEAVVACARAGKHVLCEKPPAMSL
                     AETDRMIQTCRENRVKLGCFVQCRVRGAIQAMKQAIGEGRFGRLLSADAYMKWYRSVD
                     YYKSDAWRQSRKSGAGVTVQQAFHYIDLLTYLAGPVTRVEAWMRNIAHPEIAVEDTTI
                     AHLEFASGAMGLVQASSALWPGTDVRVELNGENGTTIMSGEKMVTWRFREEKPEDGAM
                     RQLGSESQATAAGGAADLGHLDHQVVIQDMVDALREEREVIIPLATVRHTLEIVLGMY
                     QSAARRRPVELPVADDDSIWAWPESVSA"
     gene            9603..10907
                     /locus_tag="PLS90_09880"
     CDS             9603..10907
                     /locus_tag="PLS90_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02750.1"
                     /translation="MTTSQRSLETKPSARSEAASHKPPVTVVGGGMITRTQLLPTLYH
                     MQREGHLGEIHICALDSGPLREIQECPQLRRAFPGQSFTAHPDPARFSPDQKFPQLYQ
                     EVLAAAPRGSVAVIAVPDQFHYDALKAALANDLHVCVVKPMVLKYAQAVELERMAYER
                     GLVIGVEYHKRFDTRNLMARQAYREGRLGEFRLAQAQMIEPWYYRYSNFQNWCICENS
                     DMFTYVGCHYVDLVAFITGLRPVAVSVYGIVDEYPNGNKGFLWTDGRVIWENGACLSV
                     VDAIGYPNAAAGSNAQGLTMWCQGPDDAALLVHSDQYRGVKTSYTTAGSDPGDTIYCE
                     PNPDYFQYVYQGGEGLAPTGYGHRSAEHIVRACIRAGAIADLAERQRYLKQLDADGIM
                     ATPANSSYNELVIEASRLSILNGGREVVIQYGDAPGVAFRQY"
BASE COUNT         1847 a         3567 c         3705 g         1910 t
ORIGIN      
        1 ccaccgcatc cccagaagag accgccccgg agccgcaggc cggcgcggcg gaggcgcccg
       61 ccgccgcggc gagcgccgcg cccgcgcacg acgggttctt caccaaggac gtcatccgtc
      121 acaacatgat gttcctcatc atgctggagg cgatcctgat gaccggttcg gccgatttcc
      181 agatcggcct gcagcccctg ctcgaataca tcaatgcccc ctacaagctg atcggcttca
      241 ttcagggact gtcgtggacc gcgattctgg gctgcatcgc gaccccctac atcacccggc
      301 acttccccta taagaaatgg tattacatca ccgtcaccag cctgtgggcg ctcagtctgg
      361 cggtgctggg catcgcactg gtcacctcgc cggtgtggaa cccgtcactg tttgcgctca
      421 ccatcgtcac gctgatctgc gtcgtcttct tcaacatgac gatggggttt acgaatacgc
      481 cgcacaacga gttcatcgcc tcgtgtattc cacccgatca tcgcgggcgg ctgttcggca
      541 tttccacggc gctctacggc ctggtctcgc tgggggtcat cgcgatcagc cgctatctgc
      601 ttgaagtgat cgagcacccg gtcagcttcg gtttcatctt catcatgacg tgcgtcatct
      661 ggctgatcgg tttcggcttt gccagtttcg cgcgcgagat gcgcacgccg gtcgagaaat
      721 cgcccaaacc ctggaccagg gagatgttcc gcgcgttctt cctcgacaag gcctacatcc
      781 gcttcgtcat cttctgcgcg ctgacgcacc tgacgttcat gccgctgttc gtgctgatcc
      841 cgaactacgg gctgcgcgag atgaacatgc cggcgaccgc ggccgccgat atcgcgttca
      901 tcagccagat cgtcgtaatc gtcacctcga ccccgtccgg ctggctgatc gacaagttcg
      961 gaccgaagcg gatgctgccg atctccgtcg ggctgatcgg ctgcgcctac atgtttctgt
     1021 ttatcgtctt cacgccgctg gggatctact ccagcatggg gctgatgaag ctggcgctgg
     1081 cggtgttcta tccgtgcctg acgatcctgt cggtctcgat ccccaaaccg gaggatcgcg
     1141 cggggcactt ttcgacgttt tccatcgtcg ggatgctcta tttcgcggcg gggccgatca
     1201 tcggcgggtt tgtcagcgac tggatcgagc cgcggacggt cgtgctgatc ggctcgctgc
     1261 tggcattcgc gtttattccg ctgacaatcg tcctgctgcg ggttctcgcg acccatctga
     1321 gggattatta ttgacaggtc aaatttcggc ggaagggacg caagggacag gaaggacgta
     1381 agggacgcaa ggagatcatg cgtgctgccc caagggctgc cgtcagtcgg atggcgccag
     1441 gttctccggc cacctctttc aggggtttga ttcgtcgggg gcggcaatgc agggtgccgg
     1501 ccagcggccg tcaccctgcg ctttattccg gtcgtccgct ccgcggactg ctgcggtcaa
     1561 cccgtcggaa ccgcttgcat tgaatctggg attcttaaga agtgtgactt gagaatgctg
     1621 agcaatgaag tcgcgggctt cagtctgccg tccccgtcgg gacgggcctg ttgatcattc
     1681 cggcggagct gcggccgtcc acggtctcat tttcaagcca ttgtaaaccg gcggttgatg
     1741 cgccgctcgc aatcgccgct gcgcgaccgg atggcggggc ggcgggcgtg tgcgcgcaca
     1801 aaacggcttg acccctcccc aggtaactgg tagtttacct cgcattaacc gagcgtaggt
     1861 gccaccttga cgtcatcgga gcgcttcatg ccccacttgc ccgcgtccat cgacaccccg
     1921 gagcaactcg aagattgcct cagtctgccg tatgaggaaa cggttcaagc cctgcggggc
     1981 ctggacggcg acatcatggt gctcggcgcc tccggcaaga tgggcccgac ggtggcgcgc
     2041 atggcgcagc gcgcggtgcg ccaggccggc caggaccgca aggtatacgc cgtttcgcgc
     2101 aacccgtcgc gcgagctggc ggccgccggc atccacacga tcgcctgcga tctgctcgac
     2161 cccgacgcgg tcaaagagct cccccgcgcc gagaacatca tctacatggt cgggcgcaaa
     2221 ttcggttcga agggggccga gcacctgacg tgggcgacca acgtgctggc cgccggctac
     2281 gccgccgccg cgtttcccga cgcccgtttc gccgctttct ccaccggctg cgtctatccc
     2341 gtcatgcaca tcgggtcggg gggcgccacc gaggatgagc cccccgcgcc gatcggcgag
     2401 tatgcccaat cgtgcctcgg ccgcgagcgc gcattcgatt attacagcga aacccacggc
     2461 ctcaaggtcg tccatctgcg gcttaactac tcggtcgaac tgcgctacgg cgtgctcgtc
     2521 gatatcgcgc agaaggtctg ggagcagcaa ccgatcaacc tgacgaccgg ctacgcgaac
     2581 gtcatctggc agggagacgc gtgcaaccag gcgctgcagg cgctggcgct ggccgattcg
     2641 ccggccagag ttctcaatct gaccggcccc gagacgattt cgatccggcg ggtggcgctg
     2701 gaattcggca agctgatgga ccgcgagccg gtcttcgtcg gcgaggaaaa cggcttcggc
     2761 tacctgagca acgcgatgcg ggccaatcgt ctgttcggca acccgcgcgt ccccgtcggc
     2821 caggtgatcc tgtggatcgc ccactggttc cagaatcagg gagtgctgct cggcaagccg
     2881 acccagtttg aaacacagga cgggcaatac tgaggcccgt tcggttcgct ccccgcgcgg
     2941 gagccagcaa ggtgaaagat gcccgccgcc aggacacttc gcatcgggat cgtcggtctc
     3001 ggccacctgc atccccggct ctacatgccg cattttcagg cgctgcccgg cgtcgaggtg
     3061 acggccgtcg cggaaccgga tcagaggctg cgcgaagcct tcgccgcgga cttcgggctg
     3121 cgcgcctacg cctcgctgaa cgatctgctc aagcacgagt cgctggagat cgcggcgatc
     3181 ttcctgccgc acgccgagtg cgccgaggcg gcggcgcgct gcgcggcccg ggggatccat
     3241 ctgatggttg agaagccgat ggcgtcggac gtcaaggggg cgcggcggat cgtgcaggcg
     3301 gcgcgcaagg ccggcgtgca gctcacgacg ggctactgct ggcggtttca tcccgtggca
     3361 cggcgcatca agtcgttgat cgccgagggg gcaatcggcc gcgtgatcag cgccgagggg
     3421 cgttgcgccg ccggccgcgt gacgcgctac attgaagggc actcggactg gatgctcgag
     3481 aaagcgcgat cgggcggcgg cccgatcttc aatctcggcg tccattggat cgatctgatc
     3541 cgctggatgc tggcggatga agtgaccgag gccaccggcc tgaacgtcca cgtcaaccgc
     3601 cgctacgaca tcgaggatca atcctacgcg catctgcgct tcgccggcgg cgccgtgctg
     3661 gcgctcgata tcagctacac cgtgcccgac gcctatcccc atggccgcga cctgtatctg
     3721 gcgattcgcg ggaccgacgg cgtcctgtcg tggactccgg gcttcgaggg cgaaaccgaa
     3781 acgctcttcc tgtgcagcga tgccccgggg ttcgccggcg cgcccaacca gcaaatctcc
     3841 ttcgcgctcg aaccgacgcc cggctatgcc gggttcatgg gccgcgatta catcgcgcgg
     3901 ttcgtggatg cggtccgcgg aacaggcgaa ccgccggtca gcggcgagga cgccgtcgcc
     3961 gtgctgaaca tcgtgcgggc gatctacgaa tccgatgagc ggaaacagcg cgtcaaggtt
     4021 cgaaattgag gggatggctg ccatgaaagc ggtcgtgacg gactacatcg aatccgatct
     4081 ggagtgggag caggcacagc ttgcgccgca cgggatcacg ctgacggcgc atcagctgaa
     4141 gttccggccg gccgacgaag tgtatgaggc cgtcaaggac gccgacgcga tcgtcgtcaa
     4201 catggtcaag atggacgacg cgctgctgcg ccggctcgag aagtgcaagc tgctgatccg
     4261 ccacgggatc ggttacgaca acgtggacgt cgccgcctgc acgcgttacg gaatccagtt
     4321 cgcctatcag cccgattatt gccagatcga cgtggcggag cacgcggtgg cgctgatcct
     4381 gtcgctcgcg cggcggatcc cgttcagccg ggcgacgctg gagaattcga gccgcacggg
     4441 ccagtgggac ttctcggagc tctttccgat ttatcggatg gagggcaaga cgctcggcat
     4501 cgtcgggctg ggccgcatcg ggcggcgcgt cgcgcgcaag ctgtcgggct tcggcctgaa
     4561 gatgctcgcc gcgacgcgcc gcccgatgcg gcccagcnnn nnnnnnngaa gatgctcgcc
     4621 gccgacccgt atgtcgaccg cgccgtggcc gaggacctgg gggtccggct ggtgacgctg
     4681 gacgaactgc tgggcgaggc cgactacatc acgctccaca cgagcctgac cgatgagacg
     4741 cggcgcctga tcaacgccga cgccttgggg cggatgaaac cgacggcgct gctggtgaac
     4801 acgtcgcgcg ggccgatcgt tgaccaggag gcgctggccg atgcgctggc cgagggccgg
     4861 atcgccgggg cggcgatcga cgtgttcgat gtcgagccgc cgccaatcga ctatcgcctg
     4921 tttcagtccg atcgcgctat cctgacgccg cacataggct gggcctcgga agaatcggcc
     4981 tgggagatcc gccgacggat tgtggatgac ctgatcagcc acgcccaggg ccggcccgcg
     5041 cgctgcgtca tcaaccccga agtgctggcc gcccgctagc gaaagggagg cgtcaccatg
     5101 accatcacgc gagttgcggg tccgctgccg ggtcctcgcg ggcgcgagct gctggagcaa
     5161 tggcaccagt acgaggccga cgtcgtcggg tttcaggccc cggtcgtgtg ggagagcgcg
     5221 cgcggctgcg tggtgcgcga cgtcgacggc aacctgtaca tcgactggac gtcgggcgtg
     5281 ctggtgacaa atgtcgggca ttgccatccg cacctggtgc ggatgatcaa cgaggcggcg
     5341 cagcggctgc tgaataatta cgaatgcgcc aatgtggagc ggatcgaagc ggcgcggcgg
     5401 ctggtccagg cgctgccgga gcacctcgac cgctgcttct ttctgagcac gggcagcgag
     5461 gcgatcgagg ccgccggccg gctgctgaaa cggcgcaccg ggaagtttga aattctcacg
     5521 ttcgagggag gcttccacgg gcgcacgacg gcggcggccg cgttcggcgg catggccggc
     5581 cccaagcgcg gctacgggcc gacgctcccc ggcgtcattc gcgtggcgtt ccccaatccg
     5641 tatcgcgacc cgttcggctg gtgcgacgac ggcccggaat tcccccgcta tttcgactat
     5701 ctggatcagg tcgtgatggc caattccacg ggcagcctgg cgggcgtgct ggtggagccg
     5761 tatcaggggg cggccgggtt catttttccg cccgccggct ggctcaagcg cctggagcgc
     5821 tgggcgcgcg accgcaagct cctgttcgcg ctggacgagg tgcaatcctc gtatggccgg
     5881 accgggcgga tgtgggcgct cgaacacgag gatctgcggc ccgacatcgt cacgatcggc
     5941 aaggggatcg gttcgggctt cccggtctcg gccgtcgccg ccacgcatga tgttttcgcg
     6001 tgcctgtcca agggggagat gtcgagcacc atggggggca acccggtctc gagcgcggcg
     6061 gtgatcgcgg tgctggacat catggagcgg gaagatctgg tcgcccgctc cgcatcgatg
     6121 ggcgagtaca tgaagcagcg gctcgaaacg atgaaggctg cgtcgcccca cttgggcgat
     6181 gtgcgcggga tgggcctcgt gctgggcctg gaactggtca aggatcgcga atcgaagcaa
     6241 ccggcgcccg agctgacccg cccgctgatg ctggcctgcg ccgagcgcgg gctgctggtc
     6301 gggtcggtcg gcgtccatgg caacgtgatc cgggtcgccc cgccgctggt catctcgcgg
     6361 gaggaggccg acgaaagcct caacatcatg gagcaggcgc tgcgctcgct caccccggaa
     6421 catgccgccg tatgatcgcc gcgcaagcca tccacgccgc cgtcgccgag aatctccccg
     6481 aagccgtgga ccatctgtgc cggctgatgg cctttccctc gctgtcgggg caggagcacg
     6541 aggcgatgct ccatctggag caagtgttct ccgacctggg gctgaccgtc gagcgggtgc
     6601 cgatgcggcc ggatctgctc cgcgatcccg attacagcag cccgatcccg ggccttgcga
     6661 tggaggggcg gttcaacctg cgcgtggtct gccccggccg ttccgcgcgc aagatcattc
     6721 tcaatgcgca cgtcgacgtc gttccgctgg ccgaggggat ggcgtggggc agccggcgcg
     6781 agggcgagac gattttcgga cgcggcgcct gcgacgacaa ggggcccctc atcacgctct
     6841 ggctggtggc ggcgacgctg acccgcgccg gcgttgaacc gccgtgcgag atcgtgctgc
     6901 atctggtcaa cgaggaggag aacgggggca acggctcgct gcagatgatc cgccatggcg
     6961 agcgggccga cgcctgcatt gtgatggaac cgaccgaggg gcgcgtgttt tcctcaatcc
     7021 gcggggccgt ctggttccgc atcgatttcc gcggccaggc cggccacagc ggtcagccgg
     7081 gggtcacgcg cagcgccctc acgatggccc gcgaagcgat gacggcgctg gaggactacc
     7141 accgccggct gctggccgag tcggccgggc tgccgctgtt cgaagcgtat cccaatccga
     7201 tgccgctgac attcgggcgg ctggaggcgg gcaactggcc cgccgcggcg ccgcagcacg
     7261 cccggcttga gggggtgctg gggttcctgc cgaaccggac gcgcgaccag gtctgcgcgg
     7321 agttgcgcgc cgcgctgctc gatgctggcc agctcaaagc cgacgatttc gagctcggct
     7381 tcacgtatcg ccatgattgc agcatgctgg acccggagca ttttctcgcg cgcgacctcg
     7441 tgcaggccgc ccgccaggcc ggctacgatt cacgcatcga cgcaatgacc gcctcgtgcg
     7501 acgcgtggtt ctacaacaat cagctttcga tcccgacggt tgtcttcggc ccgggcagcc
     7561 tgcgctgggc gcacacgcgc gaggaacaga tcgcgatccc ggaaatcgcc cgggccgcgg
     7621 aagccattct cctttccata ctcaattcca ctggattaca ggacgacgca tgaagattgt
     7681 tcgatttgaa acaggtgaag gacgcatcca tctgggccgc cagcgggacg acggcgccgt
     7741 cgaacggctc gacggggaat tgttcgacgg gctggcgccc acgggggagg tgcggcagcc
     7801 gcgacgcatc ctcgccccgc tgatcccgac aacggtctat tgcatcgggg ccaactatcg
     7861 ccgccactgc gacgaatgca acgtcgccta tcccgaacag ccgatcgtct tcatgaaggg
     7921 ggcgaacgcg ctggtcgatc ccggacaacc gatcgtcatg cccagcgtgt gcgacggcga
     7981 tgaggtcgac tacgaaggcg agctcgccgt cgtgatcggc aagcgctgca agaatgtcag
     8041 ccgcgagcgc gcgctcgatt acgtgcttgg ctatacctgc gccaacgacg tctcggcgcg
     8101 caactggcag aagaaccgcg gcggggggca atggtgccgc ggcaaggggt tcgactcgtt
     8161 tttgccgctg ggacccgcgc tggtcaccgg cgacgagatc cccgatcccg ccgagctgac
     8221 gctgcgcacg tgggtcaacg gcgatctgcg ccaggaaagc ccgacgaacg acatgatttt
     8281 cgatatcccc gaactgatcg ccttcctcag ttcgggcacc acgctgctcc ccggaacggt
     8341 gatcctgacg ggcacgccgc acggcgtcgg cctggggttc gatcctccgc gcatgctgcg
     8401 gccgggcgat gaagtgcgga tcgagatcga tcggatcggc gcgctggtca atccggtggc
     8461 ggccgaagca caagcatgac cggaagcccg cgttccgggg gcgaggggga ggacgccatg
     8521 gcggcaaaca ctggttttgg cattcttggg gcgggactga tctcgccgct gcacgcccgc
     8581 gcgatccacg acgccgaggg ggcggacctg atcgccgtgt gcgacatgga tctggaacgc
     8641 gcccgcaaat tggccgaacc gtacggcgcc agggtttacg attcgctcga ggcgatgctg
     8701 gccgatcccg ccatcggcgt ggtctgcgtc gtcacgccga accatctcca ttgtgaagcg
     8761 gtcgtcgcct gcgcccgggc gggcaagcat gtgctctgcg agaagccgcc ggcgatgtcg
     8821 ctggcggaga ccgaccggat gatccagacg tgccgggaga atcgggtgaa actgggctgt
     8881 ttcgtccagt gccgcgtgcg cggcgcgatt caggcgatga agcaggcgat cggcgagggc
     8941 cggttcggcc ggctgctttc ggccgacgcc tatatgaaat ggtatcgctc ggtggactat
     9001 tacaaatcgg acgcgtggcg ccagtcgcgc aagtcgggcg cgggggtcac cgtgcagcag
     9061 gcctttcatt acatcgacct gctgacgtat ctggcggggc cggtcacgcg cgtcgaggcg
     9121 tggatgcgca acatcgccca tccggaaatc gccgttgagg acacgacgat cgcgcatctg
     9181 gaattcgcca gcggcgcgat ggggctggtg caggcgtcgt cggcgctgtg gcccggcacc
     9241 gatgtccgcg tcgaactgaa cggcgaaaac ggcacgacga tcatgagcgg cgagaagatg
     9301 gtcacctggc ggttccggga ggaaaagccg gaggatggcg cgatgcgcca gcttggttcc
     9361 gagtcgcagg cgacggcggc cggcggcgcc gccgatctgg gccatctcga tcatcaggtc
     9421 gtgatccagg acatggtgga cgcgctgcgc gaagagcgcg aggtgatcat cccgctggcc
     9481 accgtgcgcc atacgctgga gattgtgctg gggatgtatc aatccgccgc tcgccgccgc
     9541 ccggttgagt tgccggtggc ggacgacgat tccatctggg cgtggcccga aagcgtctcg
     9601 gcatgacaac gtcgcaacgg agccttgaaa ccaagccgtc ggcgcggagc gaagccgcgt
     9661 cgcacaaacc gccggtgacc gtcgtcggcg gcggcatgat cacgcgcacg caactgctgc
     9721 cgacgctcta tcacatgcag cgcgaggggc atctgggcga aatccatatc tgcgccctgg
     9781 attccggccc gctgcgcgag atccaggagt gtccccagct ccgccgggcg tttcccggtc
     9841 agtcgttcac cgcgcatccc gacccggccc ggttcagtcc ggatcagaag ttcccgcagc
     9901 tctaccagga agtgctggcg gcggcgccgc gcgggtcggt ggccgtgatc gccgtccccg
     9961 accagtttca ctacgacgcg ctcaaggcgg cgctcgccaa cgacctgcat gtctgcgtcg
    10021 tcaagccgat ggtgctcaag tacgcgcagg ccgtcgagct ggaacggatg gcctacgagc
    10081 gcggcctcgt catcggcgtc gaataccaca agcggttcga cacgcgcaat ctgatggccc
    10141 gccaggcgta tcgcgagggg cggcttggcg aattccgcct cgcccaggcc cagatgatcg
    10201 agccgtggta ttatcgctat tcgaattttc agaactggtg catctgcgaa aacagcgaca
    10261 tgttcaccta cgtcggctgt cattacgtcg acctggtggc gttcatcacc ggtctgcgcc
    10321 ccgtcgccgt cagcgtttac gggatcgtcg atgagtatcc caatggcaac aagggcttcc
    10381 tctggaccga tggccgggtc atctgggaga acggcgcctg cctgagcgtg gtcgacgcga
    10441 tcggttaccc gaacgccgcc gccggcagca acgcgcaggg actgacgatg tggtgccagg
    10501 gacctgatga tgccgcgctg ctggtgcaca gcgatcagta tcgcggcgtc aagaccagct
    10561 acacgacggc cggttcggac cccggcgaca cgatctactg cgagcccaat cccgattact
    10621 tccagtacgt ctatcagggg ggcgagggcc tggcgccgac cgggtacggc catcgcagcg
    10681 ccgagcatat cgtgcgcgcc tgcatccgcg ccggcgcgat cgccgatctc gccgagagac
    10741 agcgttacct caagcagctc gacgcggacg gcatcatggc gacgcccgcc aacagcagct
    10801 acaatgagct ggtcatcgag gccagccgcc tgagcatcct gaacgggggg cgggaagtcg
    10861 tcatccagta tggcgatgcc cccggcgttg ccttccgcca atactgaccc tcggcaagtt
    10921 ctggatttat gaagtaaagg agcgcccgcc atggccacca gggaattccc gctcgaaccg
    10981 gtcgaggtcg accgggtcga aacccgatgc cgccggatcg tcacgccgct gccggtgcc
//
LOCUS       DAOPCO010000126        11007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5805_length_11007_cov_4.636596, whole genome shotgun sequence.
ACCESSION   DAOPCO010000126 DAOPCO010000000
VERSION     DAOPCO010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5805_length_11007_cov_4.636596"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4894)
                     /locus_tag="PLS90_09885"
     CDS             complement(<1..4894)
                     /locus_tag="PLS90_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK02751.1"
                     /translation="MQSKLFRQATVACGWLLTGLFATFAPAATFEVIYADGENEGFFD
                     STLGDERRAAFEFALNLIADLLDSDVPIKVQASFDPMGSEETGAALGSTFPNSFHWNF
                     PGAPLPNTVYVQALANALSGTDLWDATDEEPEFDPDIVCMFNSDVDSEEEFGLADWYY
                     GLDGNAGNDYDFVTVVRHEVCHGLGFLALIDSTDGSWLLDFPDAYSVHLTRQGVGDLI
                     LMNNAQRRQAIVSNQLYWKGPAVVAEKLGQVRIYAPSPYSGGASIAHWDPVNFPDLLM
                     EPFYTVPIINTIDLTLQAFQDIGWPLIAPPEPTPTPTPTATPTPTPTPTPTPSGIPDF
                     VVDLIEPEETNINVAVPLRVELIFRNAGEGYCPITRNEIWLSPDPILTGTGNDIYVKD
                     QWVDELLAGETAEFTEANGLWVTLNLNAVGRAQNVELPPGTYYLGVLADVDDRVPNES
                     DENNIFRYHTPLTVSVDVPATPSNPKPANGDEKVAIHRSEQNFTTDVSFEWDRPANAS
                     RFDVFLSTYGEANLEFNKTDIATDALGVEDIEIVDLNDDGRLDLISTWTDAGVVAVHL
                     NRGGNPTQFETKTFPQGGRPVGLALGDINGDGIIDFVNANNLTEIYYYLNDGEENFEQ
                     HKVSLEYVLDPVSFLPIAGSIANAPRDVSVADFDLDGDMDIFVSGDGGSSLLESDLAF
                     RPEPGMDEPPPPPYPLWYGWILFDVPGFYDIESAQLNFLPPGGTPDLPWQFNDIFPDV
                     AAVSIQNDKLSVLINIGRTDRNDNPFDGPPVNFREYVIADDLDGAISLDIPDQRNWAD
                     PEIYVAAYFDQAVYRFVQNGLIPDPNGSGPNDEPTRVPLFSRQLVGGNLPDVTDVLAV
                     DLTGDGILDVVAASAAEDRIVLFESDGDSVPNYTARDLVHGEVPSDRVQEFKAGDLDG
                     DGLLDLASASNFKGAVSVFHARPTLPPESIVASNILRNGYEQFQDLLYNQIYVWQVRA
                     KAGVFRTDGPVWVFRTQPVQFMPSIETTPNAPLLVEPTGVLDITMTATNTGLSPLETR
                     TMPTDSVFYQMLLASKDQSVSLDDTVLFLASYFSYQERLSYTPTEITQADRQIPSTVR
                     ADGSVILPGSSRDQSTTLFLDLTGEPLEEGIYHLLTVVDSDDDLKEFFDAEKHIANHR
                     FMDDVYLHHEPLIVARIPDIPQGPFPPHESEGNDTQITFRWDPTGHANEYDFSLSKAS
                     GVMYEFDGRDIAITRDLRSVGAADIDGDGDLDLYSALTGVAGDQQSRIEWYRNLGGNP
                     PLFLTLMASTAINGAMEAAAADMNHDGLMDLLSASFFDNKIAWYENNGIVPPDFTQHV
                     IADNVRGAIAVFPVDMDRDGDMDVLSASALGDGIFWHERINLVEPAGFYTRNVDNTSG
                     AASDVIAADLTGNSYPDIIATYSSSDSVVWYEHSGLVPSSLVARHVITTTAEGASSVA
                     AADIDGDGDMDLVVSSEVDRTVAWYESDGQPVPTFTEHVISNSANGVRNVAIGDVDGD
                     GRLDVLAAINALDRVVYYRQIDADEVTFETHPVSEDIQRAWDVTVAPLTSSVSADVIT
                     ASAGNGKIQLFDFKRRHVFEDKLETEAEDLKTNQYFHPELLDPDSGYAWRITAKNRLT
                     TETVITTSGPIWVFSTGVPDL"
     gene            complement(4884..5501)
                     /locus_tag="PLS90_09890"
     CDS             complement(4884..5501)
                     /locus_tag="PLS90_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02752.1"
                     /translation="MDRKDTEVTMETGRIRWKGLLLAAGASLLVVSVFGWSLRPAGVA
                     AETDQTRGASSGTERETAQPAASASEAVATGAATTPTRAPRRGQGKVVVLNDRPLAKY
                     QEQKGARAGGAGFIAVLDHDGRPTVPTAGTPIPGAGLIPPPPAQIEVLASPHGGSMAK
                     GGPWVATFITAEKNEQGEFVVRCRNAACMDAEHHHEGEVRATDAE"
     gene            complement(5876..8632)
                     /locus_tag="PLS90_09895"
     CDS             complement(5876..8632)
                     /locus_tag="PLS90_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPK02753.1"
                     /translation="MALKPDSSFASEERTFSEETLDLLREILGILIGLVALLIFLSLL
                     SERQDARWIGPIGEWIAVSLDFAFGHYVSFVLPAMLAICAWHALRGLSIAPWRGRGWL
                     RGLGGLLIIISTCALLTLSTLARGEAEPTRAFAMGGVVGNLLIDAEGANLAALLGHVG
                     TAVLFGALALIGLVLFTETMLRDLATLGWGGVRRVGRGGAGPLRRLAAWHVLLPGWWG
                     RRLLGPIADAGGRLAGFHPLERLTGGLRLISFRRPERARIAGPGPIIELVRAEDARRE
                     TAHGAEAEPEDPIEAAAIASRRRGLSVFPRAAAGRGANDAGGGEPRPISALTPPTEDE
                     EASVAPEPRSSGVLARRAAQRESVAVEQHHGPDHDLERLARARVKAEQPEMDLFPSEY
                     QLPSLDLLTDPPAVEYEMSEEEQTRLSQRIEQTLAQFKVEVNVVEVIQGPVITRFALK
                     VAPGVRVSKIISLESELAMALKAPHVRILAPIPGQAAVGIEVPNRKSNPVMLKELLAS
                     EAFKTSKSPLSFALGKNIAGEPIVCDLAKMPHLLIAGATGSGKSVCLNAIIASLLYRN
                     KPDQVKFVMVDPKRVELSIYQAIPHLIAPVVSETKKAAAALAWCVEQMEKRYKLLAEL
                     GLRNIDGYNALVRDPTTNKRAYGRSDLHYMPHVVIIIDELADLMVVAKNEVEEYIIRL
                     AQMARAVGMHLVLATQRPSVNVITGIIKANFPSRIAFQVSSKVDSRTILDMNGAEALL
                     GRGDMLYSPGGAKPFRIQGCFVADAEVERLADFIREQEKARYEKEDFEARPTPAERAK
                     AQLRVEEADEAPEMEEAGGELDELAVAGRAGAAPLTPADMEQLSDEELYDMALRLILE
                     SRKASVSYIQRRMKIGYARAGRIMDILEEQGIVGPYQGSKPRDLLIDPVEYLQRLREA
                     GAED"
     gene            8859..10019
                     /locus_tag="PLS90_09900"
     CDS             8859..10019
                     /locus_tag="PLS90_09900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02754.1"
                     /translation="MGRALKIFHVNMHHHWGGQPNRILTESLGLRELGHEPWVAGPRG
                     CLLVERARAAGLKTFDDLELRRGFRPASALRDLRALTALFRRERFDIIHPHGSQDAWL
                     SLLAARRLSPRPPLVRTRHNTFPIAGHALNRWHYRQFDWVITISPQVNPLVEAVGFPA
                     EKITAVYSAPDPTRFYPREGDPRLRAELGIPAGAPVVGMVGRLAPEKGHGLILRAFAR
                     VRGRFPEARLVLVGKGRSQPEIEALAEELGIKEQVILTGFRTDVPDLVALFDIFALTP
                     ISGESLGTSILEAFCMEKPAVATEVGGTGESVRNGETGFLIPEGPEEEKIAGIADALT
                     RLLGDAELRRRMGVAGRQLVERQFTPRRLAEQCAQIYARLAQTAPENPPVGL"
     gene            complement(10108..10428)
                     /locus_tag="PLS90_09905"
     CDS             complement(10108..10428)
                     /locus_tag="PLS90_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPK02755.1"
                     /translation="MLDAARAWTSLPVSNLRAFWRIGKPELQWRGILRSSVRQRGRAS
                     VAFREAHPVIPWKAIIGLRNALIHEYGEIRFEILWSVVQQKLPELIQELHAMNIDLTR
                     AEED"
     gene            10675..>11007
                     /locus_tag="PLS90_09910"
     CDS             10675..>11007
                     /locus_tag="PLS90_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02756.1"
                     /translation="MKIYTAEAEVLQFRHVWTEMIRGIIHSRYLAYRLFLKDLRAEYS
                     RLKFSLVWDFLEPLMLALVFIALMRSGVVSDRGIAMPPAVFITFGVLLWMTFSEAVTM
                     PLGVVGKSR"
BASE COUNT         1893 a         3639 c         3501 g         1974 t
ORIGIN      
        1 ccaggtcggg aacgcccgtg ctgaagaccc agatcggtcc ggaagtcgtg atcaccgtct
       61 cggtcgttag ccgattcttc gccgtgatcc gccacgcata gcccgaatcg gggtcgagca
      121 actcggggtg gaagtactgg ttggtcttca ggtcttccgc ctcggtttcc agcttgtcct
      181 caaacacatg gcgccgcttg aagtcgaaaa gctggatctt gccgttgccc gccgaggcgg
      241 tgatgacgtc ggccgaaacg gagctggtca gcggagcgac cgtcacgtcc cacgcacgct
      301 ggatatcctc cgagaccgga tgggtttcga aggtcacctc atcggcgtcg atctgccggt
      361 agtacaccac ccgatcgagc gcattgatcg cggccagcac gtccagccgg ccatcgccgt
      421 cgacgtcgcc gatcgccaca ttccgcacgc cgttggccga gttgctgatg acatgttccg
      481 tgaacgtcgg caccggctgc ccgtccgatt cataccaggc gacggtccgg tccacttcgg
      541 acgaaaccac cagatccatg tcgccgtcgc cgtcgatgtc ggccgccgcc accgaactgg
      601 cgccttccgc cgtcgtcgtg atgacgtgac gggcgaccag cgaggagggc accagcccgc
      661 tgtgttcgta ccacacgacc gagtccgagc tggagtaggt cgcgatgatg tcgggatagg
      721 agttgccggt caggtcggcg gcgatcacat cgctggcggc gcccgaggtg ttgtccacat
      781 tgcgggtgta gaagcccgcg ggctcgacca gattgatccg ttcgtgccag aagatgccgt
      841 cgcccagggc cgaggcgctc agcacgtcca tgtcgccgtc gcggtccatg tccacgggga
      901 acacggcgat cgcgccgcgg acgttgtccg cgatgacgtg ctgcgtgaaa tcggggggca
      961 cgatgccgtt gttctcgtac caggcaatct tgttgtcaaa gaacgaggcg ctgagcagat
     1021 ccatcaggcc gtcgtgattc atgtcggcgg cggcggcttc catcgcgccg ttgatcgcgg
     1081 tcgaggccat caaggtcagg aagagcggcg ggttgccgcc cagattccga taccactcga
     1141 tgcggctctg ctggtcgccc gccaccccgg tcagggcgct gtagaggtcc agatcgccgt
     1201 cgccgtcgat gtcggcggcg ccgacgctgc gcaggtcgcg ggtgatcgcg atatcccgcc
     1261 cgtcgaactc atacatgacg ccgctcgcct tcgagagcga gaagtcgtac tcgttggcat
     1321 gtccggtcgg gtcccagcgg aacgtgatct gcgtatcgtt gccctcggat tcgtggggtg
     1381 ggaaagggcc ctggggaatg tcggggattc gcgcgacgat cagcggttca tgatgcaggt
     1441 agacgtcatc catgaaccgg tgattggcga tatgcttctc ggcgtcgaag aactccttca
     1501 ggtcatcgtc ggagtcgaca accgtcagca gatgatagat cccctcctcc agcggttcgc
     1561 cggtcaggtc caggaatagc gtggtgctct ggtcgcggct cgacccgggc aggatgacgc
     1621 tcccatcggc ccgcacggtg ctcgggattt gccggtcggc ctgcgtgatt tcggtcggcg
     1681 tgtagcttag ccgttcctga tagctgaagt aggaggccag gaacaggacc gtgtcgtcca
     1741 gcgacaccga ctgatccttg ctcgccagca gcatctgata gaacacgctg tcggtcggca
     1801 tcgtccgggt ttcaagcggc gacagcccgg tattcgtagc cgtcatcgta atatccagca
     1861 cgccggtggg ttcgaccaga agcggcgcat tgggggtcgt ttcgatcgac ggcatgaact
     1921 gcaccggctg cgtgcggaag acccacaccg ggccgtcggt ccggaagacg ccggccttcg
     1981 cccgcacctg ccagacgtaa atctggttgt acagcaggtc ctggaactgt tcgtagccgt
     2041 tgcgcagaat gttcgaggcc acgatcgatt cgggcggcaa cgtcggccgc gcatggaaca
     2101 ccgaaaccgc tcctttgaag ttcgacgcgc tggccaggtc cagcagtccg tcgccgtcca
     2161 gatcgcccgc cttgaattcc tgcacgcggt ccgacggcac ctcgccgtgg accaggtcgc
     2221 gcgccgtgta gttgggcacg ctatcgccgt cgctctcgaa caggacgatg cggtcctcgg
     2281 ccgccgacgc cgccacgaca tccaggatgc cgtcgcccgt caggtcgacg gccagcacgt
     2341 ccgtgacgtc gggcaggttg ccgccaacga gctggcgcga aaacaacggc acgcgggtcg
     2401 gttcgtcatt gggtccactg ccgttcgggt ccggaatcag gccgttctga acgaaccggt
     2461 agaccgcctg atcgaaatac gcggccacat agatctcggg atccgcccag ttgcgctgat
     2521 cgggaatgtc cagcgagatc gcgccgtcca gatcgtcggc gatgacatac tcccggaagt
     2581 tgaccggcgg tccatcgaac gggttatcgt tgcgatcggt ccggccgatg ttgatcagca
     2641 cggaaagctt gtcattctgt atcgagaccg cggcgacatc cgggaagata tcgttaaact
     2701 gccaaggcag atcgggtgtc ccgcccggcg ggaggaaatt gagctgcgcg ctttcgatgt
     2761 cgtagaatcc ggggacgtcg aagagaatcc agccgtacca cagcggatac ggcggcggcg
     2821 gcggttcatc catcccgggc tcgggacgaa acgccaggtc gctctccaac agcgaggacc
     2881 caccgtcgcc cgagacgaaa atatccatgt cgccatcgag atcgaagtcc gccacgctga
     2941 cgtcgcgcgg cgcgttggcg atcgaaccgg cgatcggcag gaagctcacg gggtcgagaa
     3001 cgtactccaa actcaccttg tgttgttcga agttctcttc cccgtcattc aggtagtagt
     3061 agatttcggt cagattgttc gcgttgacga aatcgatgat gccgtccccg ttgatgtcgc
     3121 ccaaggccag cccgacagga cggccgccct gggggaaggt cttggtctca aactgggtcg
     3181 gattgccgcc gcggttgaga tgaaccgcga cgacgccggc atcggtccag gtcgagatca
     3241 gatcgagccg gccgtcgtcg ttcagatcga cgatctcgat gtcctccacg cccagggcat
     3301 ccgtcgcgat gtccgtcttg ttgaactcga ggttcgcctc gccgtaggtc gacaggaaca
     3361 cgtcgaaccg cgaggcattc gccgggcgat cccattcaaa gctcacgtcg gtcgtaaagt
     3421 tctgctcgct gcggtggatc gcaaccttct cgtcgccgtt cgccggtttg ggattcgacg
     3481 gcgtcgccgg gacgtcgact gacaccgtga gcggcgtgtg atagcggaag atgttgttct
     3541 cgtcgctttc gttggggacg cggtcatcca catcggccag cacgcccagg tagtaggttc
     3601 ccggcggcag ttccacgttc tgcgcgcggc cgacggcgtt caggttgagc gtcacccaca
     3661 ggccgttggc ctcggtgaat tccgccgttt cgccggccag cagttcgtca acccactgat
     3721 ccttgacgta aatgtcgttg ccggttccgg tcaggatggg atcggggctg agccagatct
     3781 cgttgcgcgt gatcgggcag taaccctcgc ccgcgttgcg gaagatcagc tcgacccgca
     3841 gcgggacggc gacgttgatg ttcgtctcct ccggctcgat cagatccacg acaaaatcgg
     3901 gaatgcccga cggggtcggc gtcggcgtgg gagtgggggt cggcgtggcc gtgggcgtcg
     3961 gcgtcggcgt gggttcgggc ggagcgatca ggggccagcc gatgtcctgg aacgcctgaa
     4021 gagtcaggtc gatcgtattg atgatcggca cggtatagaa gggttccatc agcaggtcgg
     4081 ggaagttgac ggggtcccaa tgcgcaatgc tcgccccacc cgagtacggg ctcggagcgt
     4141 agattcgaac ctgaccgagc ttttccgcga caaccgccgg gcctttccaa tagagctgat
     4201 tgctgacgat cgcctgacgc cgctgggcat tattcatcaa gatcagatcg ccgacaccct
     4261 ggcgcgtcaa atggacgctg taggcgtccg ggaaatcgag cagccagctt ccatcggtcg
     4321 aatcgattag cgcaaggaag cccagtccgt ggcagacttc gtgacgcacg acggtgacga
     4381 agtcgtaatc gttgccggca ttgccgtcca gaccgtagta ccagtcggcc aggccgaact
     4441 cctcctcgga atccacatcg ctgttgaaca tgcacacaat atcaggatcg aactccggtt
     4501 cttcatccgt ggcgtcccac aggtcagtgc cggagagcgc gttcgccagg gcctgaacat
     4561 agaccgtatt cgggaggggt gcgcccggga aattccaatg gaaggagttg ggaaaggtcg
     4621 agcccagcgc cgcgccggtc tcctcgctgc ccattggatc gaacgatgcc tggactttga
     4681 tcggcacatc tgaatccagc agatccgcaa tcagattgag cgcgaattcg aacgcggcgc
     4741 gtcgttcatc gcccagcgtg gagtcaaaga agccttcgtt ttcgccgtcg gcgtaaatca
     4801 cctcgaaggt cgcggccggc gcgaatgtcg caaacagtcc tgtcaacaac cagccgcagg
     4861 cgacggttgc ctggcgaaac aacttactct gcatcggtcg cccggacctc cccctcatgg
     4921 tggtgctcgg catccatgca cgccgcattc cgacaacgga cgacgaattc cccctgctcg
     4981 ttcttttccg ccgtgatgaa cgtcgccacc caggggccgc ccttggccat cgaaccgccg
     5041 tgaggcgagg ccaggacctc gatctgggcc ggcggcggcg ggattaaccc cgcgcccgga
     5101 atgggcgtgc cggcggtggg gaccgtcggt cgcccgtcat gatccagaac cgcgatgaac
     5161 ccggcgccgc ctgcacgcgc tcccttctgt tcctggtatt tcgccagcgg acggtcgttc
     5221 agcaccacca ccttgccctg acctcgccgg ggcgcgcggg tcggggtcgt tgccgcgccg
     5281 gttgcgacgg cctcgctggc cgaggcggcg ggttgggccg tttcacgctc cgtgcccgaa
     5341 ctggccccgc gggtctggtc ggtttcggct gcgacgccag cgggccgcaa actccagccg
     5401 aagactgaga ccaccagcag gctcgccccg gccgccagca gcaacccttt ccatctgatc
     5461 cggcctgttt ccatcgtgac ttccgtatcc tttcggtcca tgcggcccaa cgtaggccga
     5521 ttccgcgggg ctcaagccga aaatcggccc cccagttcaa gccgttgcgc tagagtcgcg
     5581 catcggccgc tgttttaaaa caaaaaaagt gcacccatcc cgtccgggag gtctgatatg
     5641 gctactgtta aggggtcgaa ggcgccgccg gccgccccgc gaacgcccac ggttcttcct
     5701 cgagccggcg atcaggatgg cggcgtcagc ccggcgcgtc agcgcgcaca cacgcccttc
     5761 ctgcccttgt ccaacctgtt atatatcacc ccatcggaca tggcaatcgt caagccgccg
     5821 gcggcggagg gaccgccgga cgcgggatgg caatctcgtg atttgatcct gttgatcaat
     5881 cctcggcgcc ggcctcgcgc agccgctgca gatactcgac cggatcgatc aacagatcgc
     5941 gcggcttgga gccctggtag ggtcccacga ttccctgttc ttcgagaatg tccatgatgc
     6001 gcccggcccg cgcataaccg atcttcatcc gccgctggat gtagctgacc gacgccttgc
     6061 gcgactccag gatgaggcgc agcgccatgt cgtaaagctc ctcgtcgctc aactgctcca
     6121 tgtcggccgg ggtcagcggc gcggcgcccg cccggcccgc cacggccagt tcgtccagtt
     6181 cgccgccggc ttcttccatc tccggcgcct cgtcggcctc ctccacgcgc agttgcgcct
     6241 tggcccgctc ggccggcgtc ggccgcgcct cgaagtcttc cttctcgtag cgcgccttct
     6301 cctgctcgcg gatgaagtcg gccagccgtt cgacctccgc gtcggccacg aaacagccct
     6361 ggatgcggaa cggcttggcc ccgcccggcg agtagagcat gtcgccgcgg cccagcagcg
     6421 cctcggcccc gttcatgtcc agaatcgtgc gcgagtccac cttcgagctg acctggaacg
     6481 cgatccggct ggggaagttg gccttgatga tccccgtgat gacgttaacc gacgggcgct
     6541 gcgtcgccag caccaggtgc atccccaccg cccgcgccat ctgcgccagc cggatgatgt
     6601 attcctccac ctcgttcttg gccaccacca tcaggtcggc cagctcatcg atgatgatga
     6661 ccacgtgggg catgtagtgc aggtcgctgc ggccataggc gcgcttgttg gtggtcgggt
     6721 cgcgcaccag cgcgttatag ccgtcgatgt tgcgcagccc cagttccgcc agcagcttgt
     6781 agcgcttttc catctgctcg acgcaccagg ccagcgccgc cgccgccttt ttcgtttcgc
     6841 tcacgaccgg cgcgatcagg tgcgggatcg cctgatagat cgacagctcg acccgcttcg
     6901 ggtcgaccat cacgaacttc acctggtccg gcttgttgcg gtagagcagc gaggcgatga
     6961 tcgcgttgag gcagactgat ttgcccgagc cggtcgcccc ggcgatcagc aggtgcggca
     7021 tcttcgccag gtcgcagacg atcggctcgc cggcgatgtt cttccccagc gcgaacgaca
     7081 gcggcgactt ggacgtcttg aacgcctcgg acgccagcag ttccttgagc atcaccgggt
     7141 tggatttacg gttgggcact tcgatgccga cggccgcctg gccggggatc ggcgccagga
     7201 tccgcacgtg cggcgccttg agcgccatcg ccagctcgct ctccagcgag atgatcttgc
     7261 tgacccgcac gccgggcgcc accttcagcg cgaaccgcgt gatcaccggc ccctggatca
     7321 cttccaccac attgacttcc accttgaact gggccagcgt ctgttcgatc cgctgcgaaa
     7381 gccgcgtctg ctcttcttcc gacatctcgt attcgaccgc cggcggatcg gtcagcaggt
     7441 ccaacgacgg aagctgatac tccgagggga agaggtccat ctcgggctgc tcggccttga
     7501 cgcgcgctcg cgccagacgc tccaggtcat gatccggccc atgatgctgt tcgacggcga
     7561 cgctctcgcg ctgggcggcg cgccgcgcca gcacgccgga ggagcgcggt tccggcgcga
     7621 ccgatgcctc ctcgtcctcc gtgggtggag tcagcgcgga aatcgggcgc ggttcgccgc
     7681 ccccggcgtc gttcgcgcca cgaccggcgg ccgcgcgcgg aaagaccgac agtccgcgcc
     7741 gccgcgacgc aatcgccgcc gcctcgatcg gatcttccgg ctccgcctcc gcgccgtgcg
     7801 ccgtttcccg ccgcgcatcc tcggcgcgca ccagctctat gatcgggccc ggccccgcga
     7861 tgcgcgcccg ttccggtcgc cggaacgaaa tcagccgcag cccaccggtc agacgctcca
     7921 gaggatggaa accggcgagc cggccccccg cgtcggcgat cgggcccagc agccgccggc
     7981 cccaccagcc cggcaacaga acgtgccagg ccgcgagccg gcgcaaaggc ccggcgccgc
     8041 cgcggccgac tcgccgcacg cctccccagc ccagcgtcgc caggtcgcgc agcatcgtct
     8101 cggtgaacag taccagaccg atcaacgcca gcgccccgaa caggaccgcc gtccccacgt
     8161 ggccgagcag cgccgccaga ttggcccctt cggcgtcgat cagcaggttg ccgacgacgc
     8221 cccccatcgc gaaggcgcgg gtgggttcgg cttcgcctcg tgccagcgtg ctgagcgtca
     8281 gcagcgcgca cgtcgaaatg ataatcagca gcccgcccag gccgcgcagc catccccgcc
     8341 cccgccaggg cgcaatggag aggccgcgca gggcgtgcca ggcgcagatc gccagcatcg
     8401 ccggcagaac gaacgatacg tagtggccga aggcaaaatc gaggctgacg gcgatccact
     8461 cgccgatcgg gccgatccag cgggcgtcct ggcgctcgga gaggagcgac agaaagatca
     8521 gcagcgcgac gagaccgatc aggatgccga ggatttcgcg caggagatcg agcgtctcct
     8581 ccgaaaacgt gcgctcctcg gacgcaaacg aagagtccgg cttcagagcc atgggtcaag
     8641 accagcgtct ttccgcccgc ccggcggcgg gccagcttca acaggaacag tcagcggtca
     8701 acagaacagc aatccgaatc cgttcacaca tgaaccacat gcccccaaat ggtaggcccc
     8761 cggccataag ctcgcaagcg aaaagcgggg accgacccgg cattcgggcc cccgtcctgt
     8821 ttgcggattt gggccggttc gtctagtttg tcactgccat gggacgcgcg ctcaagatct
     8881 tccacgtcaa tatgcaccac cactgggggg ggcagcccaa ccggatcctg accgagagtc
     8941 tgggcctgcg cgagctcggc cacgagccgt gggtcgccgg gccgcggggg tgcctgttgg
     9001 tcgagcgggc gcgcgcggcc ggactgaaga cctttgacga tctggaactt aggcgggggt
     9061 tccggccggc ctcggcactg cgcgatctgc gcgcgctgac cgccctcttc cgccgcgagc
     9121 ggttcgacat catccacccg cacgggtcgc aggatgcctg gctgtcgctg ctcgcggcgc
     9181 ggcggctctc ccctcgcccg ccgcttgtgc ggacgcgaca caatacattt ccgatcgcgg
     9241 ggcacgcgct caaccgctgg cactaccgcc agttcgactg ggtgattacg atcagcccgc
     9301 aggtcaaccc gctggtcgag gcggtggggt tcccggcgga gaagatcacg gcggtttatt
     9361 cggctccgga tccgacgcgg ttctacccgc gcgaagggga tccccggctg cgcgccgaac
     9421 tgggcatccc ggccggcgcg ccggtggtgg ggatggtcgg ccggctggcc cccgagaagg
     9481 gccatggact gatcctgcgg gcgttcgccc gggtccgggg ccgattcccc gaggcgcggc
     9541 tggtgctggt gggcaagggc cgctcgcagc cggaaatcga ggcgctggcg gaggagttgg
     9601 ggataaagga gcaggtgatc ctgactggct tccggacgga cgtgccggat ctcgtcgcgc
     9661 tgttcgacat cttcgcgctg acgccgattt ccggcgaatc gctcggcacg tcgattctgg
     9721 aggcattctg catggaaaag ccggctgtcg cgaccgaagt cggcggcacg ggcgagtcgg
     9781 tccgcaacgg cgagaccggc tttctgattc ccgaagggcc cgaagaggag aaaatcgcgg
     9841 ggatcgccga cgcactgacc cggctgctgg gcgacgccga actgcggcgg cgaatgggcg
     9901 tcgccggccg gcaactggtc gaacgccagt ttaccccccg ccgcctggcg gaacagtgcg
     9961 cgcagattta tgcgcggctc gcacaaacag ccccggaaaa cccacctgtc gggttgtgag
    10021 ccctgatatt acgcccaaca atagcaacag atcctcgccg gatgtagagt ttggtcaacg
    10081 ggaatcaaac catagtttcc gtctccgtca gtcttcctca gcacgagtca ggtcaatatt
    10141 catcgcatga agttcttgaa tcaactcggg aagcttctgt tggaccaccg accacagaat
    10201 ctcaaaccgg atctccccgt actcatgaat cagggcattg cgtaggccga tgattgcctt
    10261 ccacggaatg accggatgag cttcgcgaaa ggctactgag gcgcgccccc gctgcctcac
    10321 cgatgatctc aagattcctc tccactgcaa ttctggtttt ccgatccgcc agaaagctct
    10381 caaattgctg accggcagtg aagtccaagc ccgcgcggcg tccagcatgt cccacaaccg
    10441 ggctgcttcc tgcacgctaa gcggcataaa gaacctcgcg cctgctcaag atcacgcgac
    10501 gccgacaggg gttgcggagc gcttccttct tcaccacgtc gaccgggcga cccgccatcg
    10561 cctccaaacc gtccgattcg tccactgctg tcagcacggt tgaatttcgc cccgcgcgtc
    10621 gagacaatgc cggattgtcc gttgcggcag cgcacgacgc ggaggaaacc gggcatgaag
    10681 atctacacgg ccgaagccga ggtgttgcag ttccgccacg tctggacgga aatgatccgc
    10741 ggcatcatcc acagccgtta cctggcctat cgcctgttcc tgaaagacct gcgcgcggag
    10801 tactcgcggc tgaagttttc gctggtctgg gacttcctgg agccgctgat gctggcgctg
    10861 gtgttcatcg cgctgatgcg atcgggcgtg gtgtccgacc gcggtatcgc gatgcctccg
    10921 gcggtgttca tcacgttcgg cgtgctgctc tggatgacgt tcagcgaggc ggtgacgatg
    10981 ccgctcggcg tggtcggcaa atcgcgc
//
LOCUS       DAOPCO010000127        11005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5807_length_11005_cov_6.002374, whole genome shotgun sequence.
ACCESSION   DAOPCO010000127 DAOPCO010000000
VERSION     DAOPCO010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_11005_cov_6.002374"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PLS90_09915"
     CDS             complement(<1..1258)
                     /locus_tag="PLS90_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPK02757.1"
                     /translation="MDGLNAWIATIDQWLGVISGYVWGRPMLILLVGTGIYLTILLRG
                     MQFWAMPHAFRLIFHRDEAEGDITHFSALMTALAATVGNGNIVGVAAAIALGGPGAVF
                     WMWMTGLLGMATKYAEAVLAVRYREQGPHGMCGGPMYYLAKGARLPWLGALFAVFASI
                     SAFGIGNMFQVTSVSGNLHSAFGIEPWITGVVLMVLTGLVILGGIKSIGRVSSVLVPF
                     MILGYVVAALVCLILNAERIPAALAMIFHHAFTPSAAMGGTLGALLARTISMGVARGV
                     FSNESGLGSAAIAAAAAKTKDSATQALVSMTQTFIDTIIVCTMTALVILTAPLPATFG
                     TYLNDWREMPAQVMPGTYEFVQDGATPAPGSPTPLRTKEVLTSFSMELTLGKAGAVIV
                     ALASALFAYSTLLGWSYYGEKALEYLL"
     gene            1551..2012
                     /gene="dtd"
                     /locus_tag="PLS90_09920"
     CDS             1551..2012
                     /gene="dtd"
                     /locus_tag="PLS90_09920"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPK02758.1"
                     /translation="MRVLIQRVCRASVTVDGELIGAIRRGLLLLVGIGQEDAADDGAA
                     LAPMADKILNLRILADAEGRMNRSLLDAGGEILAVSQFTLHADCRRGRRPSFTAAAPP
                     AIAARLFDDFVARLRASGLKVETGRFGAMMDVALVNDGPVTIWLDSQAQGI"
     gene            2412..3875
                     /locus_tag="PLS90_09925"
     CDS             2412..3875
                     /locus_tag="PLS90_09925"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPK02759.1"
                     /translation="MAQDAHSPVIVIGAGPAGLTAAVELARAGHRPLVLESDPHYVGG
                     LARTVQYGPYRIDIGGHRFFTRSGEVQRWWREMLPDDFIEVNRQSRIFYNGRFIDYPL
                     RLADVLRKMSPLDSAACVGSYLKRRVAPLRPEVSFRDWVVNRFGDRLFQCFFEGYTEK
                     VWGMRCTEISADWAAQRIRGLSLRRAIQNALGRNGRNGRAGTPRARSLVERFQYPRLG
                     CGMMWERARDWLERAGGDVRLDQRVTTVELEDGCVRSVHCVDSAGRPTDYRADLLVSS
                     MPLRELIEAFRPLPPAEVLHAASRLRYRDFMTVVTVIRRRECFSDNWIYINDPRVRVA
                     RIQNFRNWSPEMVPDPEVSVLGLEYFCFEHDALWSLSDAELLRLGARELERIGLAREA
                     DVIDGTVVRMTKAYPVYDHGYQDVLRVIRRWLAGIPNLRSAGRNAMHQYNNQDHSMVA
                     AMVAVRNLLGTGRVDPWDVDHEGEFSEPKPAGVASAE"
     gene            3976..5151
                     /locus_tag="PLS90_09930"
     CDS             3976..5151
                     /locus_tag="PLS90_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02760.1"
                     /translation="MSAPYTMITTPAGLADLMAALQSPAVVGLPLAIDTEADSMHHYR
                     EKVCLLQLTVDGRNWIIDPLADLDLEPLIRLLEERDLLLHGADYDLRLLGRAWRFRPR
                     RVFDTMLAAQLLGWEQFGLAALVRELCAVELPKDFQRADWSRRPLPETLLDYAAADTH
                     YLPRVAAELRRRLEKKGRLPWHAETCARLLEATARVRPDEGEPAPDAWRIKGGKHFTG
                     RAAAVLRELWLWRERTARRLDLPPFKIAGADLLLEQARWAAAHPEGGYERTPKRPAWL
                     RGARERSFEAALRRAWELPPDEFPDPPRARTGERMSPAEELLLRRAAARRDEVAARLE
                     ISPGVLAPRESLKALVRRRFELDFDADELPPLMRWQSECLRESIRCVLSDPHNPTGD"
     gene            complement(5173..6972)
                     /gene="pabB"
                     /locus_tag="PLS90_09935"
     CDS             complement(5173..6972)
                     /gene="pabB"
                     /locus_tag="PLS90_09935"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HPK02761.1"
                     /translation="MTELRGRLLLQNAGDLAPEGPAAGWWLFENPLEIVTAAEIGAVG
                     PALERIREGLRRGLFAAGFLAYEASPAFDPAFVVRAGGSGPLLWFGLYEQPTAAPEPA
                     EGSAPAALELNWRPAMAAAPYRERVGRIRELIAAGDTYQVNFTYPLEADWPESPAELF
                     ARLYDRGRARYAALVETPDHALVSLSPELFFAQAGDRVVARPMKGTIRRAPWPEADER
                     QAERLRASAKDRAENVMIVDLLRNDLGRIARPGTVRVAGLFDVERYENAWQMTSTIEA
                     RTAGDPLDVLAALFPCGSITGAPKVRTMQIIAELETAPRGVYTGAIGWIGPGRRAQFN
                     VAIRTVELRRSEAGRWQAVYGVGGGITWGSEPAAELDETRTKAAVLTPRRPPFALIES
                     LRWTPDEGYWLLDRHLRRLRRSADWFGWSCDEAEVRHALAQVERSLSGAAEPMKVRLL
                     LHPTGRIETERQPLGPVAEEVTVTPAGAPVDPRDPFLYHKTTRRGVYEEAAARRGAGV
                     DDVILWNPRGEVTEASVSNVMVRLDGRWLTPPVECGLLAGTLREELLEQGAVAEGIIR
                     LEDLARAEEIVLLNSVRGRRRARLVRHEGEGRG"
     gene            7295..8728
                     /locus_tag="PLS90_09940"
     CDS             7295..8728
                     /locus_tag="PLS90_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK02762.1"
                     /translation="MAVNITQGLLTNLIQRNLGRAAHRSEQSFERLSSGERINRAADD
                     PAGLAASDQFRYEIQSLRKLQQNVSGAFSIIGAAESQLDAITESLQRARELAVQAGSD
                     TLSPANRQAIRDELNQILAEIDRLATGTTINEFILLDGSFTNVRVQTGATAAESMTIS
                     LGDMRTGALGAWAQAASTGGVAGLPITADHLFINGVAIDPSTGDSVSSSLASASAIAK
                     AAAINAAEARTGVRAEALPTRWSGLAAIAPVNLNETTNTLRINGIAIPPVDVAPGDGA
                     LGLVNLINARSAQTGVEAALGPAGELQLTAADGRNIQLEATGAVGQALGLSATPGNVT
                     RTITGDIRLISNKAFNLNDPDGCLGIVPGVREVAVDNGLALAGIDLGSVAGAGDALRS
                     LDAALAQVGAERSRLGAMHNRLETLTGSLARRVEDLSGADSAIRDTDFAFETARLTQA
                     QILQEASLSLLAQANISPRRALELLQR"
     gene            8807..9223
                     /gene="fliS"
                     /locus_tag="PLS90_09945"
     CDS             8807..9223
                     /gene="fliS"
                     /locus_tag="PLS90_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPK02763.1"
                     /translation="MAQKLSKAYLLTKVLTASRHEVVVYLYEGAISYLHRAAQALRDQ
                     RHGHAARHVQRAISILIELSGGLDYSRGGHLALRLEAVYNHLIESLTLAAANADLEAV
                     ESCSGILSILHDAWRQASQMEHDMAATADHALRISA"
     gene            9280..9714
                     /locus_tag="PLS90_09950"
     CDS             9280..9714
                     /locus_tag="PLS90_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02764.1"
                     /translation="MFEDPGALALQLESVKQLYGELFLLLSQSIEEDPLTQATGGVDH
                     LIEPLMLTVAHEMSLLRDSVDHLRAVGRLPADLNESIEAFNGQLTEGLRDMADRIDQR
                     TRLLAAQRDEFRERLRLVQRKHQGARGYRRHAMPGAALDSEF"
     gene            10101..10595
                     /locus_tag="PLS90_09955"
     CDS             10101..10595
                     /locus_tag="PLS90_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK02765.1"
                     /translation="MHEKSIELLNKAVADELTAVHQYMFFHFHCDDQGLELLSSLFKR
                     AAIEEMLHVEKLAERILFLKGEVEMTAAAPVQKIHQVAEMLKLAARMETESARDYNKW
                     ANESAANADATTKKLFEELVMDEERHFDRFDTEAGHLDEFGDNYLALQSIERSKRGAT
                     GPHA"
BASE COUNT         1829 a         3960 c         3497 g         1719 t
ORIGIN      
        1 cgagcaggta ttcgagcgcc ttctcgccgt agtaactcca gcccagcagc gttgaatagg
       61 cgaacagcgc cgaggcgagc gccacgatga cggcccccgc cttgcccagc gtcagctcca
      121 tgctgaacga cgtcaggacc tccttggtcc ggagcggcgt ggggctgccc ggcgccggcg
      181 tcgcgccgtc ctgcacgaat tcataggtcc ccggcatgac ctgcgccggc atttcacgcc
      241 agtcgttcag ataggttccg aacgtcgccg gcagcggcgc cgtcaggatg accagtgccg
      301 tcatcgtgca gacgataatc gtatcgatga acgtctgggt catgctgaca agggcctggg
      361 tcgccgagtc tttggtcttg gcggcggccg cggcgatggc tgccgagccc agccccgatt
      421 cgtttgagaa caccccgcgc gcgaccccca tgctgattgt ccgcgccagc agcgccccga
      481 gggtcccccc catcgccgcc gacggcgtga acgcatggtg gaagatcatc gcgagggccg
      541 ccgggatccg ctccgcattg aggatcaggc agacgagggc ggccaccacg tagcccagga
      601 tcatgaacgg caccagaacg gacgagaccc ggccgatcga cttgatcccc cccaggatca
      661 ccagcccggt cagcaccatc agcacgacgc cggtgatcca cggttcgatc ccgaacgcgc
      721 tgtgcagatt gcccgagacc gacgtgacct ggaacatgtt gccgatgccg aaggccgaga
      781 tcgaggcgaa gacggcgaac agcgccccca gccacggcag gcgcgccccc tttgccaggt
      841 aatacatcgg tccgccgcac atcccatggg ggccctgttc gcggtagcgc accgccagaa
      901 ccgcctcggc atacttggtc gccatcccga gcaggccggt catccacatc cagaagacgg
      961 cgccgggtcc gcccagcgcg atcgccgccg ccaccccgac gatgttgccg ttgccgaccg
     1021 tggcggccag cgccgtcatc agcgcgctga agtgagtgat gtccccctcg gcttcatccc
     1081 ggtggaagat caggcggaag gcgtgcggca tcgcccagaa ctgcatcccg cgcagcagga
     1141 tcgtcaggta aatccccgtg ccgaccagca ggatcagcat cggccggccc cagacatagc
     1201 cggagatgac gcccagccac tggtcgatcg tggcgatcca ggcgttgagt ccatccatcg
     1261 aagttttccc ccttcgcgcc gcgccgcgcg cgccagaacg ttgcccaatt caccggctgt
     1321 tgcggacgcc tcatgttgca cgccgcgccg cgcagttgca agcgcgcccg tcggattttt
     1381 tgggaagccc cgaaaggacg gaaggaacct cggaatcaca tgcgagccgg ccgattcatc
     1441 cgcctttgcc tcttggccgt tttgcggtga ataatccgcg gggatatccg gcgggcggcc
     1501 cgggccctgg gacgccggcc gtcagcttgc ccatcatgga gggattactc atgcgcgtcc
     1561 tcattcagcg ggtctgccgg gccagcgtca cggtcgatgg cgagctcatc ggcgcgatcc
     1621 gccgcggttt gctgctcctg gtcgggatcg ggcaggagga cgccgcggat gacggcgccg
     1681 cgctcgcgcc gatggccgac aagatcctca acctgcgcat cctggccgac gcggagggcc
     1741 ggatgaaccg ctcgctgctg gatgccggcg gcgagatcct ggccgtctcg cagttcacgc
     1801 tgcatgccga ttgccgccgc gggcggcgcc cgagcttcac cgcggccgct cccccggcga
     1861 ttgccgcgcg gctgttcgac gacttcgtcg cccggctgcg cgccagcgga ctcaaagtcg
     1921 aaaccggccg gttcggcgcg atgatggacg tcgcgctcgt caacgacggc ccggtgacga
     1981 tctggctcga ttcgcaggcg caagggatct gagggcttcc acaacgagga gcagaattgg
     2041 ggcgagtctt tggggacgat cacgaggacg agcgagggat ttttgagtga ggtataaaat
     2101 gggagatgaa cggccgattc gagcgctcca cgccttgccg gatccttcgg cagggcagtc
     2161 ccgtcaggaa cggcagactt cagcccgccg ctgccagtgg cgggaactag gaacgaaaag
     2221 tcaaaagagt cccgcaaggg acggcagaac tgccccaatc gatcacgacg acgtgcggga
     2281 gacgaatgct tgagcgggcc ggaagaattg ctaattccga tttgaccatt gaccccgcta
     2341 acccgatcct caaaaatcgg tgagctcccg acggcgagcc cggtagtttg tcacccagcg
     2401 agagactccg catggcgcag gatgcgcaca gtccggtgat cgtgattggc gccggaccag
     2461 cggggttgac ggcggccgtc gagctggccc gcgccggcca tcgcccgctt gtcctggaaa
     2521 gcgacccgca ttacgtcggc ggcctggcgc gcacggtcca gtatggcccc taccgcatcg
     2581 acatcggcgg ccaccgcttc ttcacccgtt cgggcgaggt ccagcgctgg tggcgcgaga
     2641 tgctccccga cgacttcatc gaggtcaatc gccagagccg tatcttctac aacggccggt
     2701 tcatcgacta cccgctgcgc ctggccgacg tcctgcgcaa gatgtcgccg ctcgattcgg
     2761 ccgcctgcgt ggggagctac ctgaagcgcc gcgtggcgcc gctgcgcccc gaagtctcgt
     2821 ttcgcgactg ggtcgtcaac cgcttcggcg accggctgtt ccaatgcttc ttcgaaggct
     2881 acaccgagaa ggtctggggc atgcgctgca ccgagatctc cgccgactgg gcggcgcagc
     2941 ggatccgggg cctgtcgctg cgccgcgcga tccagaacgc gctcggccgc aacggccgca
     3001 atggccgcgc cggaaccccc agggcgcgct cgctcgtcga acggttccag tacccccggc
     3061 tgggctgcgg catgatgtgg gaacgcgcgc gcgattggct tgagcgggcc ggcggcgacg
     3121 tccggctcga tcagcgcgtc accaccgtcg agctggagga tggctgcgtc cggtcggtcc
     3181 actgcgtcga ttccgccggc cggccgaccg actaccgggc cgacctgttg gtcagctcga
     3241 tgccgctgcg cgagctgatc gaggcgttcc gcccgctgcc gcccgccgaa gtgctccacg
     3301 ccgcctcgcg cttgcgctac cgcgatttca tgaccgtcgt taccgtcatt cggcgccgcg
     3361 aatgcttcag cgacaactgg atttacatta acgacccgcg cgtccgggtc gcgcgcattc
     3421 agaatttccg caattggtcg cccgagatgg tccccgatcc cgaggtgtcg gtgctgggcc
     3481 tggaatattt ctgcttcgaa cacgacgcgc tgtggtcgct gtccgacgcc gagctgctgc
     3541 ggctgggcgc gcgcgaactg gagcggatcg gcctggcgcg cgaagccgac gtcatcgatg
     3601 gaaccgtcgt ccgcatgacc aaggcctacc cggtctatga ccacggctat caggatgtct
     3661 tgcgcgtgat ccggcgctgg ctggccggta tcccgaacct ccgctccgcc ggccgcaatg
     3721 ccatgcacca gtataataac caggaccact cgatggtggc ggcgatggtg gcggtgcgca
     3781 atctgctcgg cacgggccgc gtcgatccgt gggacgtgga tcacgaaggc gagttttcgg
     3841 agcccaaacc cgccggcgtt gcgtcggccg agtaggcgca aagcgccggc tccccgaccg
     3901 catccaaccg ttgatgatcc ggcccgtgct cgctaaggtg gggccaccac ccgtgtcggg
     3961 acccgccgag gaacgatgtc cgctccctat acgatgatca ccacccccgc cggtctggcc
     4021 gatctgatgg ccgccctgca gtcgcccgcc gtagtcggtt tgcccctcgc gatcgatacc
     4081 gaagccgaca gcatgcatca ctaccgcgaa aaggtgtgcc tgctgcaact gaccgtcgac
     4141 ggccgcaact ggatcatcga tccgctcgcc gatctcgatc tggaaccgct gatccgcctg
     4201 ctggaggagc gcgacctgct cctgcatggg gcggattacg atctgcggct gctcggccgg
     4261 gcatggcgct tccgcccgcg gcgcgtcttc gacaccatgc tggcggcgca gctcctgggc
     4321 tgggagcagt tcggcctggc ggcgctcgtg cgcgaactgt gcgccgtgga actccccaag
     4381 gactttcagc gcgcggattg gagccggcgc ccgctgccgg agacgctgct cgattacgcg
     4441 gccgccgaca cgcattacct gccgcgggtg gccgccgaat tgcgccgccg gcttgagaag
     4501 aaggggcgcc tgccgtggca cgcggagacg tgcgcccgcc tgctcgaggc aacggcccgc
     4561 gtgcggcccg acgagggcga gcccgccccc gacgcctggc gcatcaaggg gggcaagcac
     4621 ttcaccggcc gcgccgctgc cgtgctgcgc gagctctggc tctggcgcga gcgcacggcc
     4681 cgccgcctcg acctgccgcc gttcaaaatc gccggcgccg acctgctgct cgaacaggcc
     4741 cgctgggcgg cggcccatcc cgaggggggg tacgagcgga cgccgaagcg gcccgcctgg
     4801 ctgcgcggcg cgcgcgagcg ttcgttcgag gccgcgctgc gccgggcgtg ggaactgccc
     4861 ccggacgaat tccccgaccc gccgcgcgcg cgcacgggcg agcggatgag cccggcggaa
     4921 gaactgctcc tgcgccgggc ggcggcccgg cgcgatgaag tggccgcgcg gctcgaaatc
     4981 agccccggcg tcctcgcccc gcgcgaatcg ctcaaggcgc tggtccgccg ccggttcgag
     5041 ctcgatttcg acgccgacga gctccccccg ctgatgcgct ggcagagcga atgcctgcgc
     5101 gaatcgatcc gctgcgtcct gtccgacccg cacaacccca ccggcgattg acaaaaaccc
     5161 gtctctcgcc ccctatcctc gtccctcgcc ctcgtggcgc acgagccgcg cgcggcggcg
     5221 gccgcgaacg gaattgagca gcacgatttc ctcggcgcgc gcaagatcct ccagccggat
     5281 gatcccctcc gccacggccc cctgctcgag caattcctcg cgcaacgtcc cggccagcag
     5341 gccgcattcg accggcggcg tcagccaccg gccgtcgagc cgaaccatca cgttcgaaac
     5401 gctggcctcg gtcacctcgc cccgcggatt ccacaggatg acatcgtcga cgccggcccc
     5461 gcgccgcgcc gcggcctcct catatacccc ccgccgcgtc gtcttgtgat aaagaaacgg
     5521 gtcgcgcgga tcgaccggcg cgccggccgg cgtcaccgtc acctcctccg ccaccggccc
     5581 cagcggttgc cgttccgttt cgatgcggcc cgtcgggtgc agcagcaggc gcactttcat
     5641 cggctcggcc gccccgctca ggctccgctc gacctgggcc agcgcgtggc gcacctccgc
     5701 ttcatcacag gaccagccga accagtcggc cgaccggcgc agccggcgca ggtgccgatc
     5761 caggagccaa tagccttcgt ccggcgtcca gcgcagcgat tcgatcagtg cgaacggcgg
     5821 ccgccgcggc gtcagcaccg ccgccttggt ccgggtttca tccagttcgg cggccggctc
     5881 cgagccccag gtgatgccgc cgccgacgcc atagacggcc tgccagcgcc cggcctcgga
     5941 ccgccgcagt tcgaccgtgc ggatggcgac gttgaactgc gcccgccgtc ccggcccgat
     6001 ccagccgatg gcgccggtat agaccccgcg cggcgcggtt tccagctcgg cgatgatctg
     6061 catcgtgcgc accttgggcg cgccggtgat cgaaccgcag ggaaacagcg ccgccagcac
     6121 gtcgagcggg tcgccggccg tgcgcgcctc gatcgtcgac gtcatctgcc acgcgttctc
     6181 gtagcgctcc acgtcgaaca gcccggccac ccgcaccgtg ccgggccggg cgatgcggcc
     6241 cagatcgttg cgcagcaggt cgacgatcat cacgttctcg gcgcggtcct tggcgctggc
     6301 gcgcagccgc tcggcctgcc gctcgtcggc ctccggccac ggcgcgcggc ggatcgtgcc
     6361 cttcatcggc cgcgccacga cgcgatcgcc cgcctgggca aagaacagct ccggggagag
     6421 cgaaaccagc gcgtggtcgg gcgtctccac cagcgcagcg tagcgcgccc ggccgcggtc
     6481 atacagccgg gcgaacagct ccgcgggcga ttccggccag tcggcctcca gcggataggt
     6541 gaaattgacc tgatacgtgt cgcccgccgc gatcagctcg cggatccggc cgacccgctc
     6601 gcgatacggc gcggcggcca tcgccgggcg ccagttcagc tcgagcgccg cgggcgccga
     6661 cccctccgcc ggttccggcg ccgccgtcgg ctgctcgtac aggccgaacc acagcagcgg
     6721 cccgctcccg ccggcccgca ccacgaacgc cgggtcgaag gccggcgacg cctcgtaagc
     6781 caggaacccg gccgcgaaca acccgcggcg caacccctcc cggatgcgct cgagcgccgg
     6841 ccctaccgcc ccgatctcgg ccgccgtcac gatctcgagc gggttctcaa acagccacca
     6901 cccggccgcc ggaccctccg gcgccagatc gcccgcattc tgtagcagca gccggccgcg
     6961 cagctcggtc atcgcctgct ctcccgagtg ggcattgtgc ggattttgcc ggggaaatcc
     7021 cataccaaag ccgaggggtt cgtatgggaa aaggttgccg atttccgtca atcatttggc
     7081 gacaggttgt cggacagtcg ggcgatcgtc agaaaatcag cgctacgaat ctggcgcggc
     7141 atcattttct gagtgcccgt catccattgt aaaccattta ttttcataca tatacgatcg
     7201 atctcggtct ccatttagat cgcccgccaa atccgggcat atcgcttgcc tctgtcgcct
     7261 ccggtctgaa cggagcacct gacgcggcgg catcatggcg gtcaacatca cccagggcct
     7321 tctcacgaat ctgatccagc gcaatctggg ccgcgcggcg catcgctccg agcagtcgtt
     7381 cgagcgtctg tcgtcgggcg agcggatcaa ccgcgccgcc gacgatccgg ccggcctggc
     7441 cgccagcgac cagttccgct acgaaatcca gagtttgcgc aagctccagc agaacgtcag
     7501 cggcgcattc agcatcatcg gcgccgccga aagccagctc gatgcgatca ccgaaagcct
     7561 ccagcgcgcc cgcgaactgg ccgtccaggc cggcagcgac acgctcagtc ccgccaaccg
     7621 ccaggcgatc cgcgacgaac tgaaccagat cctggcggaa atcgaccggc tcgccaccgg
     7681 cacgacgatc aacgaattca tcctgctcga cggctccttc acgaacgtcc gcgtgcagac
     7741 cggcgccacc gccgccgaat cgatgacgat cagcctgggc gacatgcgca ccggcgcgct
     7801 gggcgcctgg gcgcaggccg cctccaccgg cggcgtcgcc ggcctcccca tcacggccga
     7861 tcatctgttc atcaacggcg tggcaatcga tccctccacc ggcgattcgg tctcctccag
     7921 cctggcctcg gcctcggcca tcgccaaggc cgccgccatc aatgcggcag aagcccgcac
     7981 cggcgtgcgc gccgaagcgc tgcccacccg ctggagcggc ctggccgcca tcgcgccggt
     8041 gaacctgaac gagacgacca acacgctgcg gatcaacggc atcgccatcc cgccggtgga
     8101 cgtcgccccc ggcgacggcg ctctggggct ggtcaacctg atcaacgcca ggtcggctca
     8161 gacgggcgtg gaagccgcac tcggcccggc cggcgaactg caactgacgg ccgccgacgg
     8221 ccgcaacatc cagcttgagg cgaccggcgc cgtcggccag gcgctcggcc tctccgccac
     8281 accgggcaat gtaactcgca cgatcaccgg cgacatccgc ctgatcagca acaaggcctt
     8341 caatctgaac gatccggacg gctgcctggg gatcgtcccc ggggtgcgcg aggtggcggt
     8401 cgacaacggc ctggcgctgg cggggattga tctgggttcg gtcgccgggg cgggcgacgc
     8461 gctgcgctcg ctcgatgccg cgctggcgca ggtcggcgcc gagcgctcgc gcctgggtgc
     8521 gatgcacaac cggcttgaaa cgctcaccgg ctcgctggcg cgccgcgtcg aggatctgag
     8581 cggggccgat tcggcgatcc gcgataccga tttcgccttc gaaacggcgc gcctgacgca
     8641 ggcgcagatc ctgcaggagg cctcgctgtc gctgctggcg caggcgaaca tctcgccccg
     8701 ccgggcgctc gaactgctcc aacgctaacc gagcccacgt aaaataccct catttgaacg
     8761 actcgaacgg acgataagac gattaaggga aaaaaggagc gcgatcatgg cacagaagct
     8821 ttccaaggcg tatctgttga ccaaagtcct gacggccagc cggcacgaag tggttgtcta
     8881 cctctacgaa ggggcgatca gctatctgca ccgggcggcg caagccctgc gcgaccagcg
     8941 ccacggccac gcggcgcgcc acgtccagcg tgcgatcagc atcctgatcg agctgtcggg
     9001 cggcctggat tattcgcgcg gcggccatct ggcgctgcgc ctggaggcgg tttacaacca
     9061 cctgatcgag tcgctgacgc tggccgcggc caacgccgac ctggaggccg tcgagtcgtg
     9121 ctcgggcatc ctctcgatct tgcacgacgc ctggcgtcag gccagccaga tggaacacga
     9181 tatggccgcc acggccgacc atgcgttgcg gatttccgcc tgaaacgcgc cgatcttgcc
     9241 gagggaggcg ccgcatgaag cggcgcgggg tgaattgcca tgtttgagga tcccggggcg
     9301 ctggccctgc aactggagtc ggtcaagcaa ctctacggcg aactcttcct cctgctgtcg
     9361 cagtcgattg aggaagatcc gctgactcag gccaccggcg gggtcgatca tctgatcgaa
     9421 ccgctgatgc tgacggtggc gcacgaaatg agcctgctgc gcgacagcgt cgaccacctg
     9481 cgcgcggtcg gtcgcttgcc cgccgatctg aatgagtcga ttgaggcgtt caacggtcaa
     9541 ctgaccgagg gcctgcgcga catggccgac cgcatcgacc agcgcacgcg cctgctcgcg
     9601 gctcagcgcg acgaatttcg cgaacggctg cgtctggtcc agcgcaaaca ccagggcgcc
     9661 cgcggctacc gccgccatgc gatgcccggc gccgcgctgg attcggaatt ctaaatcaca
     9721 gacacccgcg tcgctcggtc ccccgtccct gtgctctcct atcgttcggt ttcttgatat
     9781 ccgggcggga aggtcgcgcc gccgattcca tccatgttcc cgccgcaatg aatccggcat
     9841 ccctgtggga tgcgaacggc gctgaatcaa tcccggaccc cattttgtgc ctcgcctcgt
     9901 tgcggctttg cgatcagttc gcatcgccgc gctttccttc ctgcgaatgg aagcattcgc
     9961 attcatttcg gatttcgaaa tgcgaggttc gtgctttcat accctgatcc cgcttgacaa
    10021 gaataacaga tcctcatatc cttattatcc ccggggccct cgccccgcgt tccttgtccg
    10081 ctagaaagga cccccgcatc atgcacgaaa agagcatcga gctgctcaac aaggcggtcg
    10141 ccgacgaact gacggcggtc caccagtaca tgttcttcca tttccattgc gacgatcagg
    10201 gtctggaact gctctccagc ctgttcaagc gcgccgccat cgaggagatg ctgcacgttg
    10261 agaagctcgc cgagcgcatc ctgttcctca agggcgaggt cgaaatgacc gccgccgcgc
    10321 ctgtccagaa aatccatcag gtcgccgaaa tgctcaagct ggccgcccga atggaaaccg
    10381 aaagcgcgcg cgactacaac aagtgggcga atgaatccgc ggccaacgcc gacgccacca
    10441 ccaagaaact cttcgaggaa ctcgtgatgg acgaggagcg ccacttcgat cggttcgata
    10501 ccgaagccgg ccatctggac gaattcggcg acaactacct ggcgcttcag tcgatcgagc
    10561 ggagcaagcg cggcgcgacg ggcccccatg cctgacgcgt ccgcgcgccg cagggcggcc
    10621 gacacgatcc gcggttgatt cggccgttgt tcctatgcat ctccgccacc ggcgtgggac
    10681 cggcgccctc gccggtcctt tccccctgca tcccgcaggg atgccagaag gccgcctcga
    10741 agagccgaaa tttgaataac cccaggtgaa gcgcagcgca accgggggaa tgcccgttcc
    10801 acccccccgt ccccgaagca ggcgaatgcc ccagtccgcg cagcccgcac ccccgccgtg
    10861 acgcgcaacc acccgctacg gacggcccgg gggaccccag tgcgccattg cctcctgggc
    10921 ccgcgccagc cgcttcgcgc gctccgcccg gcgcgcctca tccgcgcgtt cgcgtcgctc
    10981 caccccccgt aaacagaacc agacc
//
LOCUS       DAOPCO010000128        10987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5822_length_10987_cov_5.452342, whole genome shotgun sequence.
ACCESSION   DAOPCO010000128 DAOPCO010000000
VERSION     DAOPCO010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5822_length_10987_cov_5.452342"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..2631
                     /locus_tag="PLS90_09960"
     CDS             115..2631
                     /locus_tag="PLS90_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02766.1"
                     /translation="MPRFVLNVPSLDHVDAALADLQARFHAALARADAPGLPLCLRAV
                     LAAVSLLCRITAIHKTLFILRHPALFPNVDPAALVGPPPPPRPPRPAGPAGLASTPLA
                     PLVAAVSPAAPPEDPAALLALAMQSVGALTRTIPAARINLVPSAPAPESSVAPGVPPA
                     ASPLRSPSGLKKQPLTPPDAPNPANTCFPPPARPTSESPAARIETRPRASPPRPFAIV
                     TGGGRGVDWHDAFTTARSKAVPLIFSRLPSPRFIAAALVLLAASTLPAAAGRLQSALP
                     DDTVVLLHVPDVPALLRQLDASPLGAFYRDPAAAEFFRPLRTRLERLQRRLDPGATLA
                     PGEILNFFTGELVVAALPPADGNDALQWAVLFDHGADPRLTARLRQGFVTAEGDITRR
                     QHAAHGATYQELRVVREEEAAIPVGKSRTGAERITGSPIARRRRVVEQVYEYAGDAVF
                     IHLRGERGWMEGLLRRHLRAEAAPAVPPPGLLEGLPAEPPLVRLYANGAALGRWWRRQ
                     IAEANGDFNLEPLRLEDLRALVADLRIAPDRLRLDGFLHAPAPRGGLARLLFLPDAAA
                     VPMADRAPAAAAAYSEMQVPLAAAWAFAWPLFGAIAPDAQRAIAGQLRGVSLRSGIDF
                     EQDLIGRLGSQLVRYLPPAPDGATPAPVCLVRVTEGEVFGPALQRFLTAGAEAYGSFF
                     VEQVDVGGRPLVTLFAGRPDVTIARAARPLFHFTWEPELWAFSLDGAALRRELERWNA
                     GASDTLAAAAEFQRARRALTLREPIGFDYARLPALTRLLEGARSTGRRGGSARMERQL
                     VDERAAPTEAVWGRYLGPFGASRRIEEDRLVIQAALLFSP"
     gene            complement(2652..3767)
                     /locus_tag="PLS90_09965"
     CDS             complement(2652..3767)
                     /locus_tag="PLS90_09965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02767.1"
                     /translation="MPARPLRVLQVTSTSLMGGAEQMVMHLVRHADPARIQPDVLTLM
                     GPGDLAVLAMQAGVESHNWALRRMADPRLLRRMQLFLRAGRYDLVHTYGLRADLATRW
                     VANGMQLPLISSISSVDPWRRRPHVWLDRLTADGVSAWVAVAEAARQSRIRREGFPPE
                     RIHVVYNGIPDRPPPDDAARRRARALLQLEADAPVLGAIGNLRPAKGYPDLFAALARL
                     RTNWPGLTCVVAGRDDSGGELPRQARDHGLEATIRWQGFMADCAPVLAAADVFVISSH
                     WEGCPVNLLEAMRAGKAIVATAVGGIPEMIEHGRDGLLVPPGRSDLLAADIGRLLADP
                     DLRAELGGAARRRFEERFTVERMVRELTEIYEAYAAG"
     gene            3902..4852
                     /locus_tag="PLS90_09970"
     CDS             3902..4852
                     /locus_tag="PLS90_09970"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPK02768.1"
                     /translation="MSDATTLSHRHLLDVESLSAREIHLILENARGFKEVFTRSIKKV
                     PALRGKTVCTLFFENSTRTRTSFELAAKRLSADTVSFSIATSSVNKGETLIDTARTIE
                     AMGIDLIVMRHPESGAPHLLSRRVRASVINAGDGTHEHPTQGLLDAYTIQEKKGGFEG
                     LKVAIVGDILHSRVARSNIQALTKLGAEVTLVGPSTLVPRTFAQFGVRVTHQLMEGIR
                     DADVINILRIQRERMNTNFFPSIREYRLLYGITRERLKSLKPDIMIMHPGPVNRGVEI
                     DQEVADGPHSVINEQVTNGVAVRMAVLYLLAGNLPSSQRD"
     gene            complement(4878..5390)
                     /locus_tag="PLS90_09975"
     CDS             complement(4878..5390)
                     /locus_tag="PLS90_09975"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HPK02769.1"
                     /translation="MDLCRYIRDVPDFPQPGILFKDITPLLADPAAFRYVIDQFVPRY
                     AARGIDRVVGIESRGFLFAAPVALALQAKFSPARKPGKLPYKTVEESYTLEYGTNTIA
                     LHEDGIGRGEKVLVVDDLIATGGTLAATCNLVEHLGGQVEEVATLIELTFLKGRDLLA
                     GRPFFTLLQF"
     gene            complement(5576..7291)
                     /locus_tag="PLS90_09980"
     CDS             complement(5576..7291)
                     /locus_tag="PLS90_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPK02770.1"
                     /translation="MTGNAREPRADGKYRVSLVSQVWSEPGRTAAAVAAWVAAAAAEG
                     ADLVALPQECIQTAGEPIPGPASQALADLARRHQVWLIGNLRERAGGRNYLTSLLFDR
                     AGRLAGRYRKSHKLPDEDIDLGDELPVFETELGRVALRIGTDRHFPDIDHVYALQGAD
                     FIFWAQAPEPIEDEYLQDAPAIGRALDYRVMIACARYAGSTPYFLPGNFPPYRGCPVG
                     RSWLIDRDGQRLACTARIGGGVATATVEPRRAPEFRCRPDSNPAFALLTQPVQPPPAR
                     NYPRRRIRLTLVEGDLGIDEIDERLDQAGRLGSDLVGLYEFEWITAHGPRPASEVLER
                     QTADAAERLRRIAARARDHAMYVVVAGVIRRRERNEALIFDRSGELLGVYTKIRTTYA
                     EQVIGQETPVFETDFGRIAVRICADEGCPELERCYSLLGADIVLSPSQSWSPDPVTRD
                     QRDCARAMDGGFFLLETTHPSSAARHRSRIIDPCGVVIARSAHHRAGLVTAEVDLDRD
                     RPLRAIRKWRPAPPIPFEDHPSWPTVSYPDELPETANDLQETIRRQRRPELYGALKQV
                     AAAVQ"
     gene            complement(7294..8289)
                     /locus_tag="PLS90_09985"
     CDS             complement(7294..8289)
                     /locus_tag="PLS90_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02771.1"
                     /translation="MMIKLGVVTVMPHGEPWAEVLRRYYPQCELTAVWDYDPEVAAKF
                     AKDYGVGRVVERVEDMVDLVDAALIPGGRVPPRAKQGVEAYIMDWVEVGPSDHLRLAE
                     PFLKAGKPVNVDKPFADSVTEAQTMIDLSRRHRAPLMSTSAIRYSPQVVSLKEIIDAG
                     ACGLGDPRTAVLIMGGAPLYWYGIHGLEALYTVFGPGVEYVLCDEACRYYGPGNPDAW
                     SGIIKWADQKLGMLHLVRDVPAEKKEPLWPTTYVLPDFLGVYYQLRVFGTTGVTDIDV
                     KGKTYYTRMLGEFIKMIETGREPIPLEQTLEVTRILLALQQAARTGGRVYVKDIK"
     gene            complement(8398..8808)
                     /locus_tag="PLS90_09990"
     CDS             complement(8398..8808)
                     /locus_tag="PLS90_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPK02772.1"
                     /translation="MKTVFADTFFYLALLSEDDASHRGAVAWAATKNRYVTTEFVLLE
                     VVDGMSAPGARGIVSQFLRTLFRNPDTRIVPASSDLLLQGLRLFEQRPDKGWSLTDCT
                     SFVVMEHEHLREVLTGDHHFAQAGFLPLLASASS"
     gene            complement(8805..9035)
                     /locus_tag="PLS90_09995"
     CDS             complement(8805..9035)
                     /locus_tag="PLS90_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02773.1"
                     /translation="MIYSGRVRNNVIVLEDGVVLPEGTPVKIEVHEPAPAPAPPGSTL
                     LDRLGDLVGSVKGLPSDFAHNHDHYIHGTPKR"
     gene            complement(9198..9947)
                     /locus_tag="PLS90_10000"
     CDS             complement(9198..9947)
                     /locus_tag="PLS90_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPK02774.1"
                     /translation="MTVYLKIWRQPGPDQPGRMVDYTLQDVSPDMSFLEMLDVLNTQL
                     IARGDEPVEFDSDCREGICGSCGMVINGIAHGPLKGVATCQLHMRSFDDGATIVIEPW
                     RARSFPVIKDLVVDRSAFDRIIISGGYVTVKTGQAPDAHAIPVPRPDQEKAMDAAACI
                     GCGACVAACPNASAALFTAAKISHLAYLPQGRVEARERALAMVRQMDVEGFGNCSNHG
                     ECEAVCPKEISISTIAKMRREYLKAVVLNPE"
     gene            complement(9979..>10987)
                     /locus_tag="PLS90_10005"
     CDS             complement(9979..>10987)
                     /locus_tag="PLS90_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPK02775.1"
                     /translation="RPPNHIPEEDRDYYLERRYPSFGNLVPRDVASRAAKAVCDEGRG
                     VGEAGLSVYLDFRDAIARLGRKAIADKYGNLFDMYLRITDENPYEVPMRIYPAVHYTM
                     GGLWVDYNLMSTIPGLYVLGEANFSDHGANRLGASALMQGLADGYFILPYTIGDYLAG
                     STFPTATVEDDEFKATRQEVLDQTERMIRNRGNKDPRTFHRELGAIMWDYVGMARNAQ
                     GLREAITKIRALREEYWQNLKVPGRPDSFNKYLEYAGRIGDYMELGELVAMDALQREE
                     SCGGHFREEYQTEEGEALRNDERFAFAAAWEYKGPNQKPELHREDLTFESVELKQRSY
                     K"
BASE COUNT         1660 a         3764 c         3823 g         1740 t
ORIGIN      
        1 ccccaaaccc aatcccaaac gcgatcccga tcccaaaccc aatcccagcc ccagccgccc
       61 cgcgcccccc aaccccggcc gcctcggacc accaacccga aagccgcccc tccaatgccc
      121 cgcttcgtcc tcaacgtccc ctcgctcgat cacgtcgacg ccgccctcgc cgacctccag
      181 gcccggttcc acgccgccct cgcccgcgcc gacgcacccg gcctcccgct ctgtctccgc
      241 gccgtcctcg cggccgtctc gctcctctgc cgcatcaccg ccatccacaa gaccctcttc
      301 atcctgcgcc atcccgccct gttccccaat gtcgaccccg ccgcgctcgt cggcccgcct
      361 ccgcccccgc gccccccgcg cccggccggc cccgcgggcc tcgcttccac cccgctcgcg
      421 ccgcttgtcg ccgccgtctc acccgccgct ccgccggaag atcccgccgc cctcctcgcg
      481 ctcgcaatgc agtccgtcgg cgctctcacc cgcacgatcc ccgccgcccg catcaatctt
      541 gtcccgtctg cccccgctcc cgaatcctcc gtggcgccgg gcgttccgcc ggccgcgtcc
      601 cccctccgct caccctccgg tttgaaaaaa caacccctca ccccgccgga tgcccccaat
      661 ccggccaata cctgttttcc cccaccggca cggcccacct cggaatcccc cgccgcgcgc
      721 atcgagaccc gcccgcgcgc ctctcctccc cgtcccttcg cgattgtgac gggcgggggg
      781 cgaggggtag actggcacga cgcgttcact accgcccgat cgaaagccgt gccgttgatc
      841 ttcagccgtc tcccctcccc ccggttcatc gcggccgcgc tggttttgct cgccgcctcc
      901 accctgccgg ccgcagcggg gcggctgcag tcggcgctgc ccgacgatac ggtcgtcctc
      961 ctgcatgtgc cggacgtccc cgcgctgctc cgccagctcg acgcgtcgcc gctgggggcg
     1021 ttctaccgcg atccggccgc cgcggagttc ttccgcccgc tccgcactcg cctggagcgg
     1081 ctccaacgcc ggctcgaccc cggggctacc cttgcgccgg gcgaaatcct gaacttcttc
     1141 accggggaac tggtcgtggc ggccctgccg ccggccgacg ggaacgacgc gctccagtgg
     1201 gcggtcctgt tcgaccacgg cgccgatccc cgcctcacgg cccggctgcg ccagggcttc
     1261 gtcaccgccg agggcgacat cacgcgccgg cagcatgcgg cccacggcgc gacctaccag
     1321 gaactgcgcg tcgtccgcga ggaggaggcc gcgatccccg tcggcaagag ccgcaccggt
     1381 gcggagcgga tcaccgggtc gcccatcgcg cgccgccgcc gcgtcgtcga acaggtctat
     1441 gaatacgccg gcgacgccgt cttcatccac ctgcgcggcg aacgcggctg gatggaaggc
     1501 ctcctgcgcc ggcacctgcg ggccgaggcg gccccggccg tgccgccccc cggattgctg
     1561 gaagggctgc ccgccgagcc gccgctcgtc cggctctatg cgaacggcgc ggcgctgggg
     1621 cgctggtggc gccgccagat cgccgaggcc aacggcgatt tcaacctcga accgctgcgg
     1681 ctggaggacc tgcgcgcgct ggtggcggac ctccgcatcg cgcccgaccg gctgcgcctc
     1741 gacggatttc tgcacgcccc cgcgccgcgc ggcggcctcg cgcgcctgct cttcctgccc
     1801 gacgccgccg ccgtaccgat ggccgaccgc gcccccgccg cggcggcggc ctacagcgaa
     1861 atgcaggttc cgctcgccgc ggcctgggcc ttcgcctggc cgctcttcgg cgcgattgcc
     1921 cccgatgccc agcgggcgat cgcgggccag ttgcgcggcg tgtcgctccg ttcgggcatc
     1981 gacttcgaac aggatctgat tggccggctg ggcagccagc tcgtgcgcta cctgcccccc
     2041 gcgcccgacg gggcgacccc ggcgcccgtc tgcctggtcc gcgtgaccga gggggaggtc
     2101 ttcggccccg cgctgcaacg attcctgacg gcgggggcgg aagcctacgg ctcgttcttc
     2161 gtcgaacagg tcgatgtcgg cgggcgcccg cttgtcacgc tcttcgccgg ccggcccgat
     2221 gtgacgattg cccgcgccgc gcgaccgctg ttccacttca cctgggaacc ggagctgtgg
     2281 gcgttcagcc tggacggcgc ggcgctgcgc cgggagctgg agcgctggaa cgccggcgcg
     2341 tccgacacgc tggccgccgc ggccgagttc cagcgcgccc ggcgggcgct cacgctgcgc
     2401 gagccgattg gcttcgatta cgcgcgcctg cccgccctca cccggttgct ggagggcgcg
     2461 cggtcgaccg gacggcgcgg cgggtcggcc cggatggagc ggcagctggt cgatgaacgg
     2521 gcggcgccga ccgaggcggt ctggggccgg tatctcggcc cgttcggcgc gagccgccgg
     2581 atcgaggagg accgccttgt cattcaggcg gcgctgctgt tcagcccgtg aacccgcggg
     2641 gcgcggcgcg atcaacccgc ggcgtaggct tcgtagatct cggtcagttc gcgcaccatc
     2701 cgctcgacgg tgaagcgttc ctcgaaccgc cggcgggcgg cgccgcccag ttcggcccgc
     2761 aggtcggggt cagcgagcag ccggccgatg tccgccgcca gcaggtcgga gcgccccggc
     2821 ggcacgagca ggccatcgcg gccgtgttcg atcatctccg ggatgccccc caccgcggtg
     2881 gccacgatgg ccttgccggc ccgcatcgcc tcgagcaggt tcaccgggca gccttcccag
     2941 tgcgaactga tgacgaagac gtcggccgcg gccaggaccg gcgcgcagtc ggccatgaag
     3001 ccctgccagc ggatcgttgc ctccagcccg tggtcgcgcg cctggcgggg cagctcgccg
     3061 ccggaatcgt cgcgcccggc gacgacgcag gtcaggccgg gccagttggt gcgcaaccgg
     3121 gcgagcgccg cgaacaggtc ggggtagccc ttcgccgggc gcaggttgcc gatggcgccg
     3181 aggacgggcg cgtcggcctc gagctggagc agcgcgcgcg cccgccggcg cgcggcgtcg
     3241 tcgggcggcg ggcgatccgg gatgccgttg taaacgacgt gaatccgctc gggcggaaac
     3301 ccctcgcgac ggatccggct ctggcgggcg gcctcggcca cggcgaccca ggcgctgacg
     3361 ccgtcggccg tcagccggtc gagccagacg tgcggccggc gccgccacgg atcgaccgac
     3421 gagatcgagg agatcaacgg cagctgcatc ccgttggcga cccagcgcgt cgccaggtcg
     3481 gcgcgcaggc cgtaggtgtg gaccagatcg tagcgcccgg cgcggagaaa aagctgcatc
     3541 cgccgcagga gccggggatc ggccatgcgc cgcagcgccc agttgtgcga ctcgacgccg
     3601 gcctgcatcg ccagcacggc caggtcgccc ggccccatca gggtgaggac gtcgggctgg
     3661 atccgggcgg ggtcggcgtg gcgcaccagg tgcatcacca tctgttcggc gccacccatc
     3721 agggaggtgg aagtcacctg cagaacgcgc aacgggcggg cgggcatcgg ggcctttcgg
     3781 gtccagaggg atgggtcgcg gccgcgaggg gcgagggcgg gcggttgcct gaccagcatg
     3841 cgcatttcgg gcggaatccg ccattgattt cccgcccacg gcttgccatg atgcgctctt
     3901 gatgagtgac gcgaccacgc tttctcaccg tcatctgctc gacgtggagt cgttgtcggc
     3961 gcgcgagatc catctgattc tggagaatgc gcgcggcttc aaggaagttt tcacccgctc
     4021 gatcaagaag gtcccggccc tgcgcggcaa gaccgtctgc acgctgtttt tcgagaactc
     4081 cacgcgcacg cgaacgtcgt tcgaactggc cgccaagcgg ctctcggccg atacggtcag
     4141 cttttcgatc gcaaccagca gcgtcaacaa gggcgagacc ctgatcgaca cggcgcggac
     4201 gatcgaggcg atggggatcg acctcattgt gatgcgccat cccgagtcgg gggcgccgca
     4261 cctgctgtcg cggcgggtcc gcgccagtgt gatcaacgcc ggcgacggca cgcacgagca
     4321 cccgacgcag gggctgctgg acgcctacac gatccaggag aaaaaggggg gcttcgaggg
     4381 gctgaaggtg gcgatcgtcg gcgacatcct gcacagccgc gtcgcgcgca gcaacatcca
     4441 ggcgctgacg aaactggggg cggaggtcac gctggtcggc ccctcgacgc tggtgccgcg
     4501 caccttcgcg cagttcggcg tccgcgtgac gcaccaactg atggaaggga tccgcgacgc
     4561 cgacgtgatc aacatcctgc ggatccagcg cgagcggatg aacaccaatt tcttcccgtc
     4621 gatccgcgag taccggttgc tctacggcat cacgcgggaa cggctcaagt cgctcaagcc
     4681 cgacatcatg atcatgcacc cggggccggt caaccgcggg gtggagatcg atcaggaagt
     4741 cgccgacgga ccgcactcgg tgatcaacga gcaggtcacc aacggcgtgg cggtgcggat
     4801 ggccgtgctc tacctgctgg cggggaatct gccatcgagc cagcgggact gaagcggcgc
     4861 cgtggcgacg gacgatttca gaactgcagc agcgtgaaga acggccggcc ggccagcagg
     4921 tcgcgcccct tgaggaacgt cagctcgatc aaggtcgcga cttcctcgac ctggccgccg
     4981 agatgctcca ccaggttgca ggtggcggcg agcgtccccc cggtcgcgat caggtcgtcg
     5041 acgacgagga ctttctcgcc gcggccgatc ccgtcctcgt gcagcgcgat cgtgttggtc
     5101 ccgtattcca gcgtgtagga ttcctcgacg gttttgtagg ggagtttgcc cggtttgcgg
     5161 gcgggggaaa acttcgcctg cagcgccagc gccaccggcg cagcgaacag gaacccgcgc
     5221 gattcgatgc cgacgacgcg gtcgatgccg cgcgcggcat agcgcgggac gaactggtcg
     5281 atcacgtagc ggaatgccgc ggggtcggcc agcagcgggg tgatgtcctt gaacaggatg
     5341 cccggctggg ggaagtcggg cacgtcgcgg atataacggc agaggtccat cgcaaggagg
     5401 ctccgggaag gtcttcctgg aagacggcgg gatgcacggc ggacagccgc acacacgatg
     5461 cgacagcgcc cggggttttt caagccaaac cgacgcaatg cacccgatga ggacgaggct
     5521 gtttgcggga agatccagtt gagtgaaaac gcgaaagcgg cgatgggcga gccggctatt
     5581 gaaccgcggc ggcgacctgc ttcagcgcgc cgtaaagttc cggccgccgc tggcgccgaa
     5641 ttgtctcctg aagatcgttt gcggtttcgg gcagctcatc cggatacgac acggtgggcc
     5701 aactgggatg atcctcgaag gggattggcg gcgcgggacg ccacttgcga atcgcgcgca
     5761 gggggcggtc gcggtccaga tccacttcgg ccgtgaccag accggcgcga tggtgcgccg
     5821 agcgcgcgat gacgacgccg caggggtcga tgatccggct gcggtggcgc gccgcgctgg
     5881 acggatgggt cgtttctagc aggaagaacc cgccgtccat ggcgcgcgcg cagtcgcgct
     5941 gatcgcgcgt gacggggtcg gggctccagc tctggctcgg cgagaggacg atgtcggcgc
     6001 cgagcaggct gtaacagcgt tcgagttcgg ggcagccttc gtcggcgcag atgcggacgg
     6061 cgatgcggcc gaaatcggtc tcgaagacgg gggtctcctg gccgatgacc tgctcggcgt
     6121 aggtcgtgcg gatcttggtg tagacgccga gcagttcgcc ggagcggtcg aagatcaggg
     6181 cctcgttgcg ttcgcggcgg cgaatgacgc cggcgacaac gacatacatg gcgtggtcgc
     6241 gggcgcgcgc ggcgatgcgc cgcagccgct cggcggcgtc cgccgtctgg cgctcaagca
     6301 cctcgctggc cggccggggc ccgtgggccg tgatccactc gaattcgtag aggccgacga
     6361 ggtccgagcc caggcggccg gcctggtcga ggcgctcatc gatctcgtcg atgcccaggt
     6421 cgccttccac gagggtgagc cggatgcggc gccgcgggta attccgcgcc ggcggcggct
     6481 ggaccggttg cgtcagcagg gcgaaggcgg gattggagtc ggggcggcag cggaactcgg
     6541 gcgctcgccg cggctcgacc gtggccgtgg cgacgccgcc gccaatccgc gcggtgcagg
     6601 ccagccgctg gccgtcgcgg tcgatcagcc aggagcgccc gaccgggcag ccgcgatagg
     6661 gggggaaatt gccgggcagg aagtacgggg tcgagccggc atagcgcgcg caggcgatca
     6721 tgacgcgata atcgagggcg cggccgatcg ccggggcatc ttgcaggtac tcgtcctcga
     6781 tcggctcggg ggcctgcgcc cagaaaatga agtcggcccc ctggagggcg tagacatgat
     6841 cgatgtcggg gaagtgccgg tcggtgccga tgcgcagggc gacgcggccg agctcggttt
     6901 cgaagaccgg caactcatct cccagatcga tgtcttcgtc ggggagcttg tgcgacttgc
     6961 ggtagcggcc cgcgagccgg ccggcgcggt cgaagagcag cgacgtgagg tagttgcgac
     7021 cgccggcgcg ttcgcgcagg ttgccgatca gccagacctg gtggcgccgc gccaggtcgg
     7081 cgagggcctg gctggccgga cccgggatgg gctcgccggc ggtctggatg cattcctggg
     7141 gcagggcgac cagatcggcg ccctcggcgg cggccgcggc gacccatgcc gccaccgccg
     7201 ccgcggtccg gcccggctcg gaccacacct gcgagacgag gctgacccga tacttgccgt
     7261 ccgcccgcgg ctcccgcgcg ttgccggtca tgctcacttg atgtccttca cgtagacgcg
     7321 gccgcccgtg cgggcggcct gctggagcgc cagcaggatc ctggtgacct cgagcgtctg
     7381 ttcgagcggg atcggttcgc gcccggtctc gatcatcttg atgaactcgc cgagcatgcg
     7441 cgtgtaatag gtctttccct tgacatcgat gtcggtcacg ccggtggttc cgaagacgcg
     7501 cagttgataa taaaccccga ggaaatcggg gaggacgtaa gtcgtcggcc agaggggctc
     7561 cttcttctcg gccgggacgt cgcgcacgag gtggagcatg ccgagtttct gatcggccca
     7621 cttgatgatg cccgaccagg cgtcgggatt gcccggtccg tagtagcggc aggcttcgtc
     7681 gcagaggaca tattcgacgc ccggaccgaa aacggtgtag agcgcctcca acccgtggat
     7741 gccgtaccag taaagggggg cgccgcccat gatcaggacg gcggtgcggg gatcgccgag
     7801 gccgcaggcg ccggcgtcga tgatctcctt gaggctgacg acctggggcg agtagcggat
     7861 cgcggaggtc gacatcagcg gcgcgcgatg gcggcgcgac aggtcgatca tcgtctgggc
     7921 ttcggtcacg gaatcggcga acggtttgtc gacgttgact ggcttgcccg ccttgaggaa
     7981 cggctcggcc aggcgcaggt gatcggaggg accgacctcg acccagtcca tgatgtaggc
     8041 ctcgacgccc tgtttggccc tgggggggac gcggccgccc gggatgagcg cggcgtcgac
     8101 cagatcgacc atgtcctcga cgcgctcgac gacgcgcccg acgccgtaat ccttcgcgaa
     8161 cttggccgcg acctcgggat cgtaatccca gacggcggtc aattcgcact gcggatagta
     8221 gcgccgcagg acctcggccc atggctcgcc atggggcatc accgtgacga ctcccagttt
     8281 gatcatcatg tcggcctttg ctggattcgc ccgtcgcgcc cgggcgcccg taggatagca
     8341 tgagcccccg cccgcggcgg attcaaattt tcaggcctcg attggaaacg agtttgatca
     8401 ggaggaagcg ctggcgagca agggcaggaa gcccgcctgg gcgaaatggt gatcgccggt
     8461 cagcacctcg cgcagatgct cgtgctccat caccacaaag gaggtgcaat ccgtcagcga
     8521 ccagcccttg tcgggtcgct gttcgaagag gcgcaggccc tgaagcagaa gatcggatga
     8581 tgctgggaca attcgagtat cagggttgcg aaagagcgtt cgcagaaatt ggctgacgat
     8641 cccacgcgcg ccgggggcgc tcatgccatc cacaacctca agcaggacga actcggtcgt
     8701 cacataccgg tttttggttg ccgcccaagc cacggctccg cggtgtgaag catcatcctc
     8761 gctcagcaac gcaaggtaga aaaacgtatc ggcaaagacg gtcttcaccg ttttggggtt
     8821 ccatgaatat agtggtcgtg attgtgggcg aaatcactcg gcaacccctt gaccgatcca
     8881 accaggtcgc caagccggtc caacaacgtg gagccaggcg gggcgggcgc cggggcgggt
     8941 tcatgcacct cgatcttgac cggcgtccct tcggggagca cgacgccgtc ttccagcaca
     9001 attacgttgt ttctgacgcg accggagtaa atcatcttgg cagcctccac ggcggcttgg
     9061 gggcagcctg gcgccgcggc cccacggctc catgttacat ccgtggcgcc ccgcgacaca
     9121 atgaatcacg ggctcaatcc agggatggca ggcaagaatt cgtcgcggcg gatcggcgcc
     9181 gccggttgga ggccggctca ctccgggttc aggacaacgg ccttcaggta ctcgcggcgc
     9241 attttcgcga tggtggaaat cgagatttcc ttggggcaga ccgcctcgca ttcgccgtga
     9301 ttcgagcagt tgccgaagcc ctcgacgtcc atctggcgca ccatggcgag cgcccgttcg
     9361 cgcgcttcga cgcgcccctg cggcaggtag gccaggtggg agattttcgc cgccgtgaac
     9421 agggcggccg aggcgttggg gcaggcggcg acgcacgcgc cgcagccaat gcacgccgcg
     9481 gcgtccatgg ccttttcctg gtcggggcgg gggaccggga tagcgtgggc gtcgggggcc
     9541 tggccggtct tcaccgttac gtaaccgccg gagatgatga tccggtcgaa cgcgctgcgg
     9601 tcgacgacga ggtccttaat gacggggaac gagcgggcgc gccacggctc gatgacgatc
     9661 gtcgcgccgt catcgaacga gcgcatgtgg agctgacagg tcgcgacgcc cttgagcggc
     9721 ccgtgcgcga tgccgttgat caccatcccg cagctgccgc agataccctc gcggcaatcg
     9781 ctgtcgaact cgacgggctc atcgccgcgg gcgatgagct gcgtgttgag gacatcgagc
     9841 atttccagga acgacatgtc gggggagaca tcctggaggg tgtaatccac catccggccg
     9901 ggctggtcgg gtcccggctg gcgccagatt ttcaagtaga cggtcatgtt cttcgccatc
     9961 gctgcgtgcc tcgcgggatt acttgtagct gcgctgtttg agttcgacgg attcgaaggt
    10021 cagatcttcc cggtgcaact cgggcttctg gttgggcccc ttgtattccc aggcggcggc
    10081 aaaggcgaag cgctcgtcat tgcgcagggc ctcgccctcc tcggtctggt attcctcgcg
    10141 gaaatggccg ccgcacgatt cctcgcgctg gagggcgtcc atcgcgacca gttcgcccag
    10201 ttccatgtag tcgccgatgc gcccggcata ctcgaggtat ttgttgaagc tgtcggggcg
    10261 gccggggacc ttgaggttct gccagtattc ctcgcgcagt gcgcggattt tggtgatcgc
    10321 ttcgcgcagc ccctgcgcgt tgcgggccat gccgacgtag tcccacatga tcgcgcccag
    10381 ttcgcggtgg aacgtccgcg gatccttgtt gccgcgattg cggatcatcc gctcggtctg
    10441 gtcgaggact tcctggcggg tggccttgaa ttcatcgtcc tcgaccgtcg cggtggggaa
    10501 ggtcgagccg gccaggtagt cgccgatcgt gtaggggagg atgaagtagc cgtcggccag
    10561 gccctgcatc agggcgctgg cgccgaggcg gttggcgccg tgatcggaga agttcgcctc
    10621 acccaggacg tagagtccgg ggatggtgct catcaggttg tagtcgaccc acaggccgcc
    10681 catcgtgtaa tgcacggcgg ggtagatgcg catcggcacc tcgtaggggt tctcatcggt
    10741 gatgcgcagg tacatgtcga agaggttgcc gtatttgtcg gcgatcgcct tgcggccgag
    10801 gcgggcgatc gcgtcgcgga agtcgaggta gaccgagagg ccggcctcgc ccacgccgcg
    10861 gccttcgtcg cagacggcct tggccgcgcg cgaggccacg tcgcgcggaa ccaggttgcc
    10921 gaagctcgga tagcgccgct cgaggtagta atcgcgatcc tcctcgggga tgtggttcgg
    10981 cgggcgc
//
LOCUS       DAOPCO010000129        10928 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5864_length_10928_cov_5.677090, whole genome shotgun sequence.
ACCESSION   DAOPCO010000129 DAOPCO010000000
VERSION     DAOPCO010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5864_length_10928_cov_5.677090"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /gene="dapB"
                     /locus_tag="PLS90_10010"
     CDS             complement(<1..552)
                     /gene="dapB"
                     /locus_tag="PLS90_10010"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPK02776.1"
                     /translation="MTNPLPILVYGAAGRMGRMIVALAAAEPAIYRLAGAIERADHPD
                     LGQPMARLVPGAPADLALSAAIPADAPAGAVAIDFSLPEGTLAHLADCRRRGHALVIG
                     TTGFSDAQRETIRAAGAEIAVLLTPNTSLGVNVLFWLARHAARLLGPEYEIEIVEMHH
                     HHKKDAPSGTARRLAEVVAETRGA"
     gene            879..2111
                     /locus_tag="PLS90_10015"
     CDS             879..2111
                     /locus_tag="PLS90_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK02777.1"
                     /translation="MPEFLYEALDKGGKQVKGIIEASSEEIIYEKLRDMGYYPVDVRV
                     HKKKSAAQSDISALPILRIIFHRIKTKHIMTFTRQLATLIDAGLPIMRSLNILREQVE
                     SVIFKEKIMEMARDIESGSSLSDAMAKHHKVFDKLYVNMVRAGELGGVLEQVLNRIAE
                     FLEKREALKGKVKSAMMYPVVVMILAIGIVGFILIMIIPKFEAIFAELGADLPVPTQI
                     LVDLSYILVHRIWIPIVIVVAIVIIWKQMKKYPNSKYVLDRTMLKMPVFGDLFRKIAI
                     TRFAGTLSTLINAGVPILQSLDIVRDSSGNEVIARAMSKVYQSVKDGETIHEPLSECD
                     VFPPLVVHMVAVGEETGAIDHMLTKVAEAYEREVDDTVNALTSILEPVLIVFLGAIVG
                     AIVIALYLPLFSIPKLIK"
     gene            complement(2104..2394)
                     /locus_tag="PLS90_10020"
     CDS             complement(2104..2394)
                     /locus_tag="PLS90_10020"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK02778.1"
                     /translation="MEERGMHQAELARAMHVSRQHVRELLREEYNYTIRLVIRLVSPA
                     EAAPVIPVELLPEVRALLGRGASEPKRTHQKPKGETGARRRAVANRRAGAVT"
     gene            2543..3472
                     /locus_tag="PLS90_10025"
     CDS             2543..3472
                     /locus_tag="PLS90_10025"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPK02779.1"
                     /translation="MPTPRTPTLPPLGAHMSIAGGMPRAVERALAVGATALQIFLKNN
                     NQWRGKLIDDDEAAAFRAAVAAARLPPVVGHSSYLINLASPGADIARKSVESMTDEIV
                     RAGRLGVAGIVMHPGAHLGAGEQAGIELIARRINAIFAATPGNPAGIWLETTAGQGSH
                     LGWRFEHLRDILERVEDRSRVAVCLDTCHVFAAGHDIRTADGVRRMLAEFDRVIGLIW
                     LRAIHANDSAKPLGSRRDRHAHIGQGEIGEAGFAALLRDRRLRKIPFLLETPKDDAGD
                     LDRMNLATLRRLAASPPPAAATRRRKTEGPAKR"
     gene            3469..4413
                     /locus_tag="PLS90_10030"
     CDS             3469..4413
                     /locus_tag="PLS90_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02780.1"
                     /translation="MNPTGFMAIDTGSNATKYRLWRIDPSGHAEPVDAGRTPLRLGRG
                     AFTQGRLEPEMMQKAEEVFVGLLKLARRHGVEPIRAVGTSALRDAANAADLVGRIRKA
                     TGIDIRVISALEEARLIMEGVLAAPGVRAPGVGQYLLIDIGGGSTEVIATRPDKPSHL
                     LSLPLGAVRLTGQFFPTLPSSGEQVAQAARHVERTLARAGNLPPAAGERRLLGGGGSI
                     TTLYRMYSPGAPIAPPSPPDLTRDQIDELIEEITPLAPEEIVQRYQIEEDRAEVILGG
                     AVVARQLLIQLGADRVTVVWGGLCDGLLQDFLRVPAGG"
     gene            complement(4415..5077)
                     /locus_tag="PLS90_10035"
     CDS             complement(4415..5077)
                     /locus_tag="PLS90_10035"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPK02781.1"
                     /translation="MHWIHDLVDWYFHQINTMGLELYVFVGMTIESSFIPFPSEVIMP
                     PAGHQAGSFLRVIWLILIGTLGSLVGAWVNYFLGLKLGRPFFEKYGKYVLISERSLTR
                     MDHYWERYGEASTFIGRLVPAVRQLISIPAGVARMNFWRFTFYSGLGAGLWVTVLALL
                     GWFLRDWTLERFETELKERMLPYVIVGLVVLIGGYILKLQLQKRRRRRAQQPPAETHV
                     SE"
     gene            5340..6638
                     /locus_tag="PLS90_10040"
     CDS             5340..6638
                     /locus_tag="PLS90_10040"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017926365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPK02782.1"
                     /translation="MGTTATSIPSARADHAATIDAMVRKARAAARLLATCPTTTKNAA
                     LRAMARSFRDHADEIKSENARDLAAGRDAGLTPAMLDRLELNDKRIEAMAAALETVAG
                     LEDPVGEINDVRVRPCRVRIGQMRVPIGVIGIIYESRPNVTADAGCLCLKSGNAVILR
                     GGKEAFHSNTAIARLMDAAARETGLPDGSIQLVPTTDRAAVGELLRRNDCVDLIIPRG
                     GKSLIEMVVENSTIPVIKHYDGNCFIYVDRAADPAMAREIILNAKCQRPGVCNAVESL
                     LFHVDAAERIGGEVLRNLLAQGVEIRADESLRRLLPDLDLKPAADADWETEYLDLIVT
                     AGVVGSAREAIDFINIHGSHHTDAIITDDYATGMEFLRAVDSACVFINCSTRFSDGGE
                     FGMGCEIGISTDKLHARGPMGLKELTTLKFVAFGEGQVRT"
     gene            6642..9140
                     /locus_tag="PLS90_10045"
     CDS             6642..9140
                     /locus_tag="PLS90_10045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK02783.1"
                     /translation="MSASPEPAVAAPPPRAVYAALLLTTLATLLLQLGLTRLFSVLMY
                     YHMAFFAVSVTLFGIALGGVIVHFLPRLFTVEATPAWMRRMALAAAAATAAALVVLLA
                     IPFNLASNTRLIGQMMLVSLVLAVPFTCTGVAVALALTRYPRRTNLLYGFDLIGAAAG
                     CLLFPPLITWLGGPRFVLAVALLLAAAGLVLAASGAPRRRTGPALTVAVGLALILLLL
                     LAAAPRLDFLKLRHVRGQGIHPDDLEYEGWNTISRITAYSEAPPPGEPSSQPPQKTIL
                     IDTYAATPIIPFDGEHFTSVQFAFHDISYPVHALLDHARVAVIGTGGGRDVLAAKAWG
                     QPEVVGIEINSRTLEALTEVFAEFAGHPLRWPGVTLVLDEARSVIARSPRPFDIIQAS
                     LIDTFAATAAGAFVLTENSLYTLEGWHTFLDKLTPRGILTMSRWYTAPADAGGNACPV
                     ETIRLLTLARATLEQRGAARPRDHIMMARSPRPDAPGHMSLATILVAKEPFSSEQVTT
                     FEQWVRNNGMIEMVTPNRIADPLLAPVLEAPDLAAFAADYPFDISPPTDDKPFFFDVL
                     RWRDIFRREFRQGSDYIYAINLKPIIMLGTLLATVVAMAFAFIVVPLGIEGRLRQRRM
                     PAHLAPGRRLSLVAYFVMLGLGYLMIELSLMQRFTVFLGHPAYSLTVCLFTMLLASGL
                     GSMAAPRVFRAASWTNAARSIRLLNLVLFGVLLATLAGSLWMMQRFVASPTPVRILLA
                     GAAAAPAAFLMGMPFPLAIQAAARRPNAPLSWYWGINGAFSVCSSVLVVALAHTIGLS
                     GTFIVGAGCYLAAAAVAWAFGPTPAEQAPPPPNI"
     gene            complement(9292..10833)
                     /locus_tag="PLS90_10050"
     CDS             complement(9292..10833)
                     /locus_tag="PLS90_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02784.1"
                     /translation="MTSKSGESGARRQLTIVLGAGAVLVYYLALALTRRIDADEGYYL
                     YAARLVLEGRLPYRDFFYPQMPLLPYFWAVAGIAPGGVGWLGGRVLAAGVAWACALLF
                     GALLARRVRARGAFLAVGAVYLVSDFGLEWAMTAKTYGPATALLLGAWLALGRAPSES
                     GRFDRRAALAGFLVSLAVLARLTVAPAALVLPIVYLGLDWPKAASRKRWLIGYLNGLL
                     PAAIVVGVFRALAPEAFVFDNWTYHRLGGPPAAERLRANLTVLLQMLISPLWLLAWAP
                     AIAWIAASRRRPDRNTSCYAALAGVFIIVSTIPIRAFHQYYAMATPWLLLLAAPALEW
                     AWGGVARRIGRARAAAAAVAMVMGLSLFQAQTVFARRWPTWAWRPETPEAAAEHDHRL
                     GVARAVARELNARARPDDRLLTWWPGYGLEAAPPMVEGMENHFAVRVAKYVEAERARR
                     LGALPAEEIERMITGRRVALVVVGLWTGAESWLGREHYTEMLDRAGYRKVWESGSAAI
                     YEVPR"
BASE COUNT         1795 a         4175 c         3261 g         1697 t
ORIGIN      
        1 agcaccccgc gtctcggcca cgacctccgc caggcgccgg gcggtcccgc tgggtgcgtc
       61 tttcttatgg tggtgatgca tctcgacgat ttcaatctcg tattccggcc caagcagccg
      121 cgcggcgtgg cgcgccagcc agaacagcac gttcaccccc aggctcgtat tgggcgtcag
      181 cagcaccgca atctccgctc cggcggcgcg gatcgtctcg cgctgcgcgt cgctgaaccc
      241 ggtcgtcccg atcacaagcg catgaccccg ccgccggcaa tcggccaggt gcgccagggt
      301 cccctccggc agcgagaaat cgatcgccac cgccccggcg ggggcgtcgg cggggatcgc
      361 cgccgacaac gccaggtcgg ccggagcgcc cggcacgagc cgcgccatag gctgccccag
      421 atcggggtgg tccgcccgct cgatcgcccc ggccagccgg tagatggccg gctccgcggc
      481 ggccagcgcg acgatcatcc ggcccatccg gccggcggcg ccatagacga ggattggcag
      541 cgggttggtc atggcggagc gcatccgtgt tcgcgggttt gatacggcgc ggcggccctc
      601 cccgggcgcg ccgcggccag accagctttg cccaaaaggt ctcaaaagac aaccccgcag
      661 tgggtcgtta ccgccagcgc taatctgtcg ggtccagcag atccgaccga tccgactgat
      721 ttcccgcgcc gacctttgtg ctggcgctcg gccggcccgc cccggcgaaa agacatttcc
      781 ctcccgcgtt gcatagccta tacttgcaat caggccgggc cgatgcgccc ggcgcgccgg
      841 acgccgcgtt gcggccgaac ccatccggga gcagggccat gccggaattc ctctacgaag
      901 ccctcgacaa gggcggcaag caggtcaagg gaatcatcga ggccagcagc gaggaaatca
      961 tctacgaaaa actgcgcgac atgggttatt acccggtcga tgtgcgcgtt cataaaaaga
     1021 agagcgcggc gcagtccgac atctccgccc tccccatcct gcgcatcatc ttccaccgca
     1081 tcaagaccaa gcacatcatg accttcacgc gccagctcgc gaccctgatc gacgccggcc
     1141 tgccgatcat gcgctcgctc aacatcctgc gcgaacaggt cgaatcggtc atcttcaagg
     1201 aaaaaatcat ggagatggcg cgcgacatcg agtcgggctc ctccctctcc gacgcgatgg
     1261 ccaagcatca caaggtcttc gacaagctct acgtcaacat ggtccgcgcc ggcgagctcg
     1321 gcggcgtgct ggagcaggtc ctcaaccgca tcgccgagtt cctcgaaaag cgcgaggccc
     1381 tcaagggcaa ggtcaagtcg gcgatgatgt accccgtcgt cgtgatgatc ctcgccatcg
     1441 gcatcgtcgg cttcatcctc atcatgatca tccccaagtt cgaggcaatc tttgccgaac
     1501 tcggcgccga tctgcccgtc ccgacccaga tcctggtcga tctctcctac atcctcgtcc
     1561 accgcatctg gatcccgatc gtgatcgtcg tcgctatcgt catcatctgg aagcagatga
     1621 agaaataccc caactcaaaa tacgtcctcg accgcacaat gctcaagatg ccggtcttcg
     1681 gcgacctctt ccgcaagatc gcgatcactc gcttcgccgg caccctctcc accctgatca
     1741 acgccggcgt cccgatcctc cagtcgctcg atatcgtccg cgactcctcg ggcaacgagg
     1801 tcatcgcccg cgcaatgtcc aaggtctacc agagcgtcaa ggacggcgaa acgatccacg
     1861 aacccttgag cgaatgcgat gtcttccccc cgctcgtcgt ccacatggtc gccgtcggcg
     1921 aggagaccgg cgccatcgac cacatgctga ccaaggtcgc cgaggcctat gagcgcgagg
     1981 tcgacgacac cgtcaacgcg ctgacctcga tcctcgagcc ggtcctgatc gtcttcctcg
     2041 gcgccatcgt cggcgccatc gtcatcgcgc tctatctgcc gctcttcagc atcccgaaac
     2101 tgatcaagtg accgcccctg cccggcggtt cgctaccgct cgccgtcgcg cgcccgtttc
     2161 ccccttcggt ttctgatggg tgcgcttcgg ctcggaggcg cctctcccaa gcagggcccg
     2221 aacctccggt aacagttcca ccggaattac tggcgcggct tcggcgggcg aaaccagacg
     2281 aatgacgagc cgaatcgtat agttgtattc ctcgcgaagc agctcgcgca cgtgctggcg
     2341 cgaaacatgc atcgcccggg cgagctccgc ctggtgcatt ccacgttcct ccatcgcggc
     2401 caggacgtcc tcaacgagtc ttatgacacg aaatctgtcc gatccgtccg atccgtcgga
     2461 gccgtcggat ccgccggatt tcagctgaga cgctcgcgcc catctctgct atgctggccc
     2521 aacccccgac ggagggcccc cgatgccgac accgcgaacc ccgacgctcc cgccgctcgg
     2581 cgctcacatg tcgattgccg gcgggatgcc gcgcgccgtc gagcgcgcgc tggccgtcgg
     2641 cgccaccgcg ctccagatct tcctcaagaa caacaaccag tggcgcggca agctcatcga
     2701 cgacgacgag gccgcggcgt tccgcgcggc cgtcgcggcc gcccgcctcc cccccgtcgt
     2761 cggccacagc agttacctga tcaacctcgc ctcgcccggc gccgatatcg cccgaaagtc
     2821 cgtcgaatcc atgaccgacg aaattgtccg cgccggccgc ctgggcgtcg cgggcatcgt
     2881 catgcatccc ggcgcgcacc tcggcgccgg cgagcaagcc ggcatcgagc tgatcgcccg
     2941 ccgcatcaat gcgatcttcg cggcgacgcc cggcaacccc gccggcatct ggctcgaaac
     3001 gaccgccggc cagggcagcc atctcggctg gcgcttcgag cacctgcgcg acatcctgga
     3061 acgcgtcgag gaccgctcgc gcgtcgccgt ctgcctcgac acctgccacg tcttcgccgc
     3121 cggccacgac atccgcacgg ccgacggcgt acgccggatg ctcgccgagt tcgaccgggt
     3181 gatcggcctg atctggctgc gagcgatcca cgccaacgat tcggccaagc cgctcggctc
     3241 gcgccgcgac cgccacgccc acatcggcca gggcgagatc ggcgaggccg gcttcgccgc
     3301 cctgctgcgc gaccgccgcc tgcgcaagat cccgttcctg ctcgaaacgc cgaaggacga
     3361 cgccggcgac ttggaccgga tgaatctggc gacgctgcgg cgcctggcgg cctcgccccc
     3421 gccggcggcg gccacccggc gccgcaaaac cgagggcccg gccaagcgat gaaccccacc
     3481 ggcttcatgg ccatcgatac cgggagcaac gccaccaagt accggttgtg gcgcatcgac
     3541 ccgtccggcc acgccgaacc ggtcgacgcc ggccggacgc ccctgcgtct ggggcgcggc
     3601 gcgttcactc agggccggct cgaaccggag atgatgcaga aagccgagga ggtcttcgtc
     3661 ggtctgctca agctggcgcg ccgccacggc gtcgagccga tccgcgccgt cggtaccagt
     3721 gcgctgcgcg acgccgccaa cgccgccgac ctcgtcggcc gcatccgcaa ggcgaccggc
     3781 atcgatatcc gcgtcatctc cgcgctggag gaagcgcggc tgatcatgga gggcgtcctg
     3841 gcggccccgg gcgtccgcgc ccccggcgtc ggtcagtatt tgctcatcga catcggcggc
     3901 ggctcgaccg aagtgatcgc gacgcgcccg gacaagccgt cccacctgct cagcctgccc
     3961 ctcggcgcgg tgcgcctcac cggccagttc tttccgaccc tgccctccag cggggaacag
     4021 gtcgcgcagg cggcgcgcca cgtcgaacgc accctcgcgc gcgcggggaa cctcccgccg
     4081 gccgccggcg agcgccgact cctcggcggc ggcggctcca tcacgacgct ctaccgcatg
     4141 tattcgcccg gcgcgccgat cgccccgccg tcgccccccg acctgacgcg ggaccagatc
     4201 gacgaactga tcgaggagat cacgccgctc gcgccggagg aaatcgtcca gcgctatcag
     4261 atcgaggagg atcgcgccga ggtgatcctg gggggcgccg tcgtcgcgcg ccaactcctc
     4321 attcagctcg gcgcggaccg cgtcaccgtc gtctggggcg gcctgtgcga cggcctgctg
     4381 caggatttct tacgggtgcc ggccggcggt tgaatcactc cgaaacgtgc gtttcggcgg
     4441 gcggctgctg cgcccgtcgc cggcgccgct tctggagctg cagcttgaga atatagccgc
     4501 cgatcagcac aaccagccca acgatcacgt acggcagcat ccgttccttc agttcggtct
     4561 cgaaccgctc cagcgtccag tcgcgcagaa accagcccag cagcgccagc acggtgaccc
     4621 acagcccggc ccccagcccg ctgtagaacg tgaagcgcca gaaattcatc cgcgcgacgc
     4681 ccgccgggat cgagatgagc tggcgcaccg ctggcaccag ccgcccgatg aacgtgctcg
     4741 cctcgccgta gcgctcccag tagtgatcca tccgcgtcag gctcctctcg ctgatcagga
     4801 cgtatttgcc gtatttctcg aagaacggcc ggccgagctt gagcccgaga aaatagttga
     4861 cccacgcgcc gaccaggctc cccagcgtcc cgatgaggat cagccagatg acgcgcagga
     4921 atgagcccgc ctgatgcccg gccggcggca tgatgacctc gctcgggaac ggaatgaacg
     4981 agctctcgat cgtcatgccg acgaagacat acagctccag ccccatcgtg ttgatctgat
     5041 ggaaatacca gtcgacgaga tcgtggatcc agtgcacgcg gcgatccttt gccggctgcg
     5101 gtggcgcgct acaacgcgcg cgcagagatt ctaacagaag accgccggaa ttcgagcaaa
     5161 agcgcgaggc cctgacaccc gcaccgggcc tcggcccggt gaccggacgc gacgggtctt
     5221 cccaaccgtt tcaccggttt actccgtgct cccatcgcgc gtcgatttct caaatcaggg
     5281 tggaactatc ccgcgccgcg ggtatattgg aagggattgc catgagggcg aggaacacga
     5341 tgggaacgac tgcgacctcc attccatccg cccgcgccga tcacgccgcc acgatcgacg
     5401 cgatggtccg caaggcccgc gcggcggccc ggctgctggc gacctgcccc accaccacca
     5461 agaacgccgc gctgcgggcg atggcgcgct cgttccgcga ccatgccgac gaaatcaaat
     5521 ccgaaaacgc cagggatctg gcggccggcc gcgacgccgg cctcaccccg gccatgctcg
     5581 accggctgga gctgaacgac aagcggatcg aggcgatggc ggcggcgctg gaaaccgtcg
     5641 ccggcctgga ggacccggtc ggcgaaatca acgacgtgcg cgtccgcccc tgccgcgtcc
     5701 gcatcggcca gatgcgcgtc ccgatcggcg tcatcggcat catctatgaa tcccgcccca
     5761 acgtcacggc cgatgccggc tgcctttgcc tcaagtcggg caacgccgtg atcctgcgcg
     5821 gcggcaagga ggcgttccat tccaacacgg ccatcgcgcg cctgatggac gccgccgccc
     5881 gcgagaccgg cctgcccgac gggagcatcc agctcgtccc gacaaccgat cgcgcggcgg
     5941 tgggcgaact gctccggcgc aacgattgcg tcgacctgat catcccgcgc ggcggcaaaa
     6001 gcctgatcga gatggtcgtc gaaaattcca ccatccccgt catcaagcat tacgacggca
     6061 actgcttcat ctatgtcgat cgcgcggccg accccgccat ggcccgcgaa atcatcctca
     6121 acgccaaatg ccagcgcccg ggcgtgtgca acgcggtcga atcgctcctg tttcacgtcg
     6181 atgcggccga acggatcggc ggcgaagtcc tgcgcaatct gctcgcccag ggggtcgaaa
     6241 tccgcgccga cgagagcctg cgccgcctgc tgcccgacct cgatctcaaa cccgccgccg
     6301 atgccgactg ggagaccgaa tacctcgacc tcatcgtgac ggccggcgtc gtcggctcgg
     6361 cgcgcgaagc aatcgatttc atcaacatcc acggttcgca tcacaccgac gcgatcatca
     6421 ccgacgatta cgccaccggc atggaattcc tccgcgccgt cgattcggcc tgcgtcttta
     6481 tcaactgctc cacccgcttc agcgacggcg gggagttcgg catgggctgc gagatcggca
     6541 tctccaccga caagctgcac gcgcgcggcc cgatgggcct caaggaattg accacgctca
     6601 agtttgtcgc ctttggcgag gggcaggtcc gcacctgatc catgtccgct tcccctgaac
     6661 ccgccgtcgc cgcgccgccg ccgcgcgcgg tctatgccgc cctgctgctc accacgctgg
     6721 ccacgctgct cctgcaactg gggctgacgc gcctgttcag cgtcctgatg tattaccaca
     6781 tggccttctt cgccgtttcg gtcacgctgt tcggcatcgc gctgggcggc gtcatcgtgc
     6841 acttcctccc gcgcctgttc accgtcgaag ccacgcccgc ctggatgcgg cgcatggcgc
     6901 tcgccgccgc cgccgccacc gccgccgcgc tcgtcgtgct gctcgcgatc cccttcaatc
     6961 tcgccagcaa cacgcggctg atcggccaga tgatgctcgt cagcctcgtc ctggccgtgc
     7021 cgttcacctg caccggcgtg gccgtcgcgc tcgccctgac gcgctacccc cgccggacga
     7081 atctgctcta cggcttcgac ctgatcggcg cggcggccgg ctgcctgctc ttccccccgc
     7141 tgattacctg gttgggcgga ccgcgcttcg tcctcgccgt cgcgctgctg ctggccgcgg
     7201 ccggcctggt gctggcggcg tccggcgcgc cgcgccggcg aaccggcccc gcgctgaccg
     7261 tcgccgtcgg cctcgcgctg atcctcctgc ttctgctcgc ggccgccccg cgcctcgact
     7321 tcctcaagct gcgccatgtc cgcggccagg ggattcaccc cgacgacctc gaatacgagg
     7381 gctggaacac gatctcgcgg atcaccgcct acagcgaggc gccgccgccc ggcgaaccct
     7441 ccagccagcc cccgcaaaag acaatcctga tcgacaccta cgccgccacg cccatcatcc
     7501 ctttcgatgg ggaacacttc acttcggtcc agttcgcgtt ccacgacatc agctacccgg
     7561 tccacgcgct cctcgatcac gcgcgcgtgg ccgtcatcgg caccggcggc ggccgcgacg
     7621 tcctcgccgc caaggcctgg ggccagcccg aagtcgtcgg catcgaaatc aactcgcgta
     7681 cactcgaggc gctgaccgag gtcttcgccg agttcgcggg ccacccgctc cgctggcccg
     7741 gcgtaacgct cgtcctcgac gaggcgcgca gcgtcatcgc ccgcagcccg cggcccttcg
     7801 acatcatcca ggcctcgctg atcgacacct tcgcggcgac ggccgccggc gcctttgtcc
     7861 tgaccgagaa ctccctttac acgctcgaag gttggcacac gttcctcgac aagctgaccc
     7921 cgcgcggcat cctgacgatg agccgctggt ataccgcccc cgccgacgcg ggcgggaacg
     7981 cctgtcccgt cgaaacgatc cgcctgctga cgctggcgcg cgcgacgctc gagcagcgcg
     8041 gcgcggcgcg gccccgcgac cacatcatga tggcgcgctc gccccgcccc gacgcccccg
     8101 gccacatgtc gctggcgacg atcctggtgg cgaaggagcc gttcagcagc gagcaagtca
     8161 cgacttttga acaatgggtg cgcaacaacg gcatgatcga aatggtgacg ccgaaccgga
     8221 tcgccgaccc gctgctcgcg cccgtcctgg aggcccccga tctggcggcg ttcgcggccg
     8281 actatccctt cgacatctcg ccccccaccg acgacaagcc gttcttcttc gacgtcctgc
     8341 gctggcgcga tatcttccgc cgcgaattcc gccagggcag cgattacatc tatgcgatca
     8401 atctcaagcc gatcatcatg ctcggtacgc tgctggccac cgtcgtcgcc atggcgttcg
     8461 ccttcatcgt cgtgccgctc gggatcgagg ggcggctccg gcaacgccgc atgcccgccc
     8521 atctcgcgcc gggccgccgg ctgagcctcg tcgcctattt cgtcatgctc ggcctcggct
     8581 acctgatgat cgagctgtcg ctgatgcagc gtttcaccgt cttcctcggc cacccggcct
     8641 acagcctgac ggtctgcctg ttcacgatgc tgctggccag cgggctgggg tcgatggcgg
     8701 ccccgcgcgt cttccgggcc gcatcatgga cgaacgccgc gcgctcgatc cgcctgctga
     8761 acctcgtcct gttcggcgtg ctgctggcga cgctcgccgg ctcgctgtgg atgatgcagc
     8821 gcttcgtcgc ctcccccacc ccggtgcgga ttctgctcgc gggcgccgcc gccgcgcccg
     8881 cggccttcct gatgggcatg cccttcccgc tggcgattca ggccgccgcg cggcgaccca
     8941 atgcccccct cagttggtac tggggcatca acggcgcctt ctcggtctgc tcgtcggtcc
     9001 tggtcgtcgc gctcgcgcac accatcggcc tgagcgggac attcatcgtc ggcgccggct
     9061 gctacctggc cgccgccgcc gtggcctggg ccttcggccc caccccggcc gaacaagccc
     9121 ccccgccccc caacatctga tccccacatc ttcgcacgac cgataaccct ccctttgctg
     9181 cctctccgtg aactcctgga gtttatcggt ccacgcttgc ggcgatgccg cgccagatct
     9241 tatctgccct ttcgcgtctt tcgtggtcct gagtcgcctt ctcccctctc ctcaccgcgg
     9301 gacttcgtaa atcgccgcgc ttccgctctc ccagaccttc cgatacccgg cacgatccag
     9361 catctccgtg taatgctcgc ggccgagcca tgactcggcg cctgtccata gcccgaccac
     9421 caccaacgcg acccgccgcc cggtgatcat ccgctcaatc tcctcggccg gcagcgcgcc
     9481 gagccggcgc gcgcgctccg cttccacata cttcgccacg cgcaccgcga agtggttctc
     9541 catcccctcc accatcggcg gcgccgcctc aagaccgtag cccggccacc acgtcagcag
     9601 gcggtcgtcc ggccgcgcgc gcgcgttgag ctcccgcgct acggcgcgcg ccacgccgag
     9661 ccggtgatca tgttccgcgg cggcttccgg cgtctcgggc cgccaggccc acgtcggcca
     9721 gcgccgcgcg aacaccgtct gcgcctgaaa cagcgacagc cccatcacca tcgccacggc
     9781 tgccgccgcg gcgcgcgccc gcccgatccg ccgcgcaacc ccgccccagg cccattccag
     9841 cgccggcgcg gccagcagca acagccacgg cgtcgccatc gcgtaatact ggtgaaatgc
     9901 ccggatcgga atcgtcgaaa cgatgatgaa cacgccggcg agcgccgcgt aacagcttgt
     9961 gttgcggtcc ggccgccgcc ggctcgccgc aatccaggcg atggccggcg cccacgccag
    10021 cagccacagc ggactgatca gcatttgcaa gaggacggtc aggttggcgc gcaaccgctc
    10081 ggcggccggc ggcccgccca ggcggtgata cgtccagttg tcgaacacaa acgcctcggg
    10141 cgcgagcgcc cggaacacgc cgacaacgat cgcggcgggc agcagaccgt tgagataacc
    10201 gatgagccat cgcttgcgcg aagcggcttt cggccagtcg agccccagat agacaatcgg
    10261 caacaccagc gcggcggggg cgaccgtcag ccgcgcgagc accgcgagcg aaacgaggaa
    10321 tccggccagc gccgcgcggc gatcgaacct cccgctttcg ctcggcgcgc gccccagcgc
    10381 cagccacgcc cccagcagca gcgccgtcgc cgggccgtac gtcttggccg tcattgccca
    10441 ttccagcccg aaatccgaca ccagatagac cgcgccgacg gccaggaagg ccccgcgcgc
    10501 gcgaacacgc cgggcgagca gcgcgccgaa caacaacgca cacgcccacg ccacgcccgc
    10561 cgccagaacg cgcccgccca gccagcccac cccgccggga gcaatgcccg ccaccgccca
    10621 gaagtaaggc agcagcggca tctgcggata gaaaaaatcc cggtagggca gccgcccctc
    10681 cagcaccagc cgcgccgcat aaagataata accttcatcg gcatcaatcc gtcgcgtcag
    10741 cgccagcgca aggtagtaaa ccagcacagc gcccgcgccg agaacaatcg taagctgacg
    10801 tctcgcgccg gattcgccgg atttggatgt cactggtccc acggcaggca tcatagaagc
    10861 cggccgcaga ccttgcaatg tcccgtcatg ccgaggcgga gcgggcgagt cattggcgcc
    10921 gcgatata
//
LOCUS       DAOPCO010000130        10923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5868_length_10923_cov_4.515826, whole genome shotgun sequence.
ACCESSION   DAOPCO010000130 DAOPCO010000000
VERSION     DAOPCO010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10923
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5868_length_10923_cov_4.515826"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..60
                     /locus_tag="PLS90_10055"
                     /pseudo
     CDS             11..60
                     /locus_tag="PLS90_10055"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            192..593
                     /locus_tag="PLS90_10060"
     CDS             192..593
                     /locus_tag="PLS90_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02785.1"
                     /translation="MVDHTKFPVQWHGAPVQYPEILSSPIAYVASTYTFQDVFEFHEA
                     TGIDPYWRLYGYWCFQSYYELFQNTIMEDYRNHPRVNSYLLYSKGPDRILGYFNFYGP
                     YGKGTDGKAVPAVYDPTNGTISSGDICVWGP"
     gene            847..3180
                     /locus_tag="PLS90_10065"
     CDS             847..3180
                     /locus_tag="PLS90_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02786.1"
                     /translation="MSSPQIELSGPWELTHFPQESLDPEVVLGGGRGLCWIAAEVPGN
                     VVLDLHRAGQIDDPYVGMNPEQVRPFEYHEWWYRTTFTVNTPAPAGGKVNLVFEGIDT
                     FAEIYLDGRLLGRTENMFVPHRFPLAPTPKSGATHTLHVRIKSDQYEVARRDFNGVVE
                     LIGNDPSRVWIRKAAYTHGWDIFPRLLSAGIWKRVCLEYQPAYFIDDVFVFTRRFDRA
                     ASSIMARVYIRSPRAYRTDLAVRLRVREAGRVVHEATHAVQETMVCFAFELERPRLWW
                     PRPYGEPFLYQVRAELLAGDEVIDGWDGQFGVRSVEIAQEPLADGRQSFYFKINDEPI
                     SIYGTNHVPLDSCPSRYAQRLQPALELLDDSRCNMVRVWGGGVYETPEFYAACARMGV
                     MVWQDFMFACALYPQEEAFLAQVHEEARHAIRSLRNHPSVVLYCGDNEIELHLSQYFG
                     PHQLGAGNRQIANALLPELVMRHDGTRPYIPSSPFTPIAGEPPYSVKGGDTHIWCHGT
                     YFRAPVYSEDLSPFISEIGHLSLAARESIEKILPAEAVWPRDERLWSYRAGCLNDPAF
                     YTYPHGWVEVIDRNITNMFGELPAGLDDRIFASQVVQAEAYKAWVERARRRKFDCGGI
                     LWWNLLDGCPLISNAVVDFYFRKTIAYEYIRRAQDDLLFCIEKMDGELELFLVSDSLE
                     EREVSYQVVSGDEIIAQGLASVPANGVVALGSAGKAAGAARLFLLRAEAGDRAYFNHY
                     LDGEPPFDLDRVKGFHARIEAEARRAAPTLERIGAAR"
     gene            complement(3243..4346)
                     /locus_tag="PLS90_10070"
     CDS             complement(3243..4346)
                     /locus_tag="PLS90_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015819569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPK02787.1"
                     /translation="MSPLSIKGADSCRWDAIALGEIMIRLDPGEGRVRTARTFHAWEG
                     GGEYNVIRGLRRCFGLRTAVVTAIADNDIGRLLEDLILQGGVDTRYIQWVPFDGIGRR
                     VRVGLNFTERGFGVRAARGTNDRGHTAASQMKPGQVDWERVFKHDGARWFHTGGIFAG
                     LSETTPDVMIEAMEAARAAGTMISYDLNYRASLWKDIGGQARAREVNQRVAHLVDVMI
                     GNEEDFSAALGYEVEGADLEKSSELDPAAFQQMIRRVVKDYPNFKAVATTLRNAKTAT
                     VNDWSAVLYCDGEFYKATDRPNLEIFDRVGGGDSFASGLIYGFMTGKSPQQAVEYGAA
                     HGALAMTTPGDTTMASLDEVEKLIKGGSARIAR"
     gene            complement(4396..5295)
                     /locus_tag="PLS90_10075"
     CDS             complement(4396..5295)
                     /locus_tag="PLS90_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPK02788.1"
                     /translation="MRSISHTLCALLALAGLAVAGCPRDKAAGLAAADSAATAPLTDQ
                     TQAEPSPANPTPTNPNPPAPALTPPAPDIAAPLTDEQKALNAGLLFNLGLQERPDTNT
                     SVEAPRIAITNHGVQIVGDAAVFDGAEEDHLLLAAAPTAGLSEYTIAMRIELAAMPEI
                     PQPFYAEFTDRSRPPRLILSVQGASDLPRLACELRGSTKVHSLIHPLTAEQAGGPFTL
                     ALVVNPESIALYVDGALADEVANAPDDTPESDGVTVIGAAAPGGRVDPAYRSFFNGKI
                     YAVRAYNRALSPAEVARLEPLLK"
     gene            complement(5438..6655)
                     /locus_tag="PLS90_10080"
     CDS             complement(5438..6655)
                     /locus_tag="PLS90_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02789.1"
                     /translation="MRSLSSRRSAPAMVACLAILFLGLATSSLHALTSTADLDFPSVN
                     GPIYGVAQGGDTILIGGAFSVVDGVGRARIAALDAYSGQLWDWNPNITDGFVYRIHIV
                     GDKVYVGGSFSDVNGVARNRMAAFNLPTHAADPAVLDPAWDPDVNGTIDEVASSGGKI
                     YICGNFTTVSGVTRNYAAALSPANGVDAVTVDPWHPDFGGRCRALLPHGNRVYCGGPF
                     TTVKGGTARNRLAAFEPADGVNTAVLDEAWSPNVGATAVYCLASDGVHLYLGGDFIST
                     GGNSNHGILSAVELANGLNTGAVIADWDPPLIFPLVRRILIVDEFVIALGSFVLSDHN
                     QYLVAFQRAALGYTLDPWAPTPNGVVYDGTLSGAGFLVAGGFTTMEGVHAPGKIAGFN
                     STVPVRFRAYTAE"
     gene            complement(6880..7941)
                     /locus_tag="PLS90_10085"
     CDS             complement(6880..7941)
                     /locus_tag="PLS90_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HPK02790.1"
                     /translation="MSIRLTGITRSFGSFKALDAVNLTVEEGELLALLGPSGSGKTTL
                     LRIIAGLDACDPDRGGTIEVNGQDVSAMRVGERKVGFVFQHYALFRHMSVFENVAFGL
                     RVLPRKDRPPRNEIARRVRELLRLVQLDKLEQHRPDQLSGGQRQRVALARALAIQPRV
                     LLLDEPFGALDARIRQELRRWLRRLHDELHMTSIFVTHDQEEALEVADRVVIMNEGRI
                     EQVGAPADVYHHPATPFVYTFLGDVNLFHERSEAGAALVALPTPDSRSTPYPGAAFAR
                     PHQVDLLPPDQTDLGVEGIVRRIHAAGPMVKVHLMTADGGDVLAVLTQERFAQLGVAP
                     GMRLRAVPRELRAFTAAAG"
     gene            complement(7938..8810)
                     /gene="cysW"
                     /locus_tag="PLS90_10090"
     CDS             complement(7938..8810)
                     /gene="cysW"
                     /locus_tag="PLS90_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HPK02791.1"
                     /translation="MSIAVSHHPGLARARFRPVRATLIAVTLAFLALLLVLPLAVVLV
                     EALRMGLATCLHAITEPAALAAIRLTLLTAGVALLANGLFGLAAAWAIARFEFRGKSL
                     LLSLIDLPFAVSPVISGLVFILLFGLHGWLGQWLAAHEIRIIFAVPGILLATVFVTFP
                     FVARELIPLMQEQGSEQEQAALVLGAGPWQTFWRVTLPNIKWGLLYGLILCNARAMGE
                     FGAVSVVSGHIRGRTNTLPLHVEVLYNEYNFAAAFSVASLLILLALVTLVLKTALEWK
                     VARGIAPRPRGGHS"
     gene            complement(8807..9685)
                     /gene="cysT"
                     /locus_tag="PLS90_10095"
     CDS             complement(8807..9685)
                     /gene="cysT"
                     /locus_tag="PLS90_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HPK02792.1"
                     /translation="MSAAAAPLLPRLLGRSAAPAALPGFGLSLGFTLFYLSLIVLLPL
                     SALFFKAGTLGWEEFWRATVNPRVLASYRLTFTTAFVAAAVNALFGLIVAWSLVRYRF
                     PGRRLADALIDLPFALPTAVSGIALTTLYSSHGWVGRWLEPLGIRAAYSPLGITIALT
                     FIGLPFVVRTLQPVIEGLEREQEEAAVSLGANPWQTLRRVLFPVIAPALLTGFALAFA
                     RGLGEYGSVIFISGNLPYRTEIASLLIVTRLEEYDYAGATAIAVVMLLASFALMLGIN
                     ALQHWSARRLGQGGAA"
     gene            complement(9686..10822)
                     /locus_tag="PLS90_10100"
     CDS             complement(9686..10822)
                     /locus_tag="PLS90_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPK02793.1"
                     /translation="MNPYPVADPIASRLAPRPGAAPRLRLRAAAVSLLLSFLAALTAP
                     LAPAADALELLNVSYDPTREFYEAFNAAFGEHWAAEHGGAIAVQQSHGGSGKQARSVI
                     DGLEADVVTLALAHDIDSIAKRSGLLPADWQSRLPHQSCPYTSTIVFLVRKGNPRGIA
                     DWDDLIQPGIEVITPNPKTSGGACWNYLAAWGFALRRELGDLNRLHDPAAAAEVEQAQ
                     AKARQFVAELFTHVPVLDTGARGATNTFVQRRLGDVLLAWENEAFLALEQFGRDDYEI
                     VVPSISILAEPPVAWIDANTERHGTTDAARAYLEFLYSDVGQRLAARHFYRPVLSEKA
                     DPQDLARFPELEMFTIDDVFGGWSRAHAEHFADGAIFDQIQRRP"
BASE COUNT         1939 a         3134 c         4082 g         1768 t
ORIGIN      
        1 tgaaatcaag atgaaggcaa ctcgcggctt cacactgatt gaattgctca tcgtcgttgc
       61 gatcattgca attctggccg ccatcgcagt tccgaacttt ctcgaagcac agacaagatc
      121 taaggtaagc cgcgccgtag cggacttacg tactctcaga atcgctgtgg aatcatattg
      181 tggaatcata tatggtggac cacacgaaat ttccagtcca atggcacggt gctcccgtgc
      241 agtatcccga gattctgagc agtcccattg cctacgtagc ttcgacttac acgttccagg
      301 acgtgttcga gttccatgaa gctacgggca tcgaccccta ctggaggctt tacggctact
      361 ggtgtttcca gtcttactat gagcttttcc aaaatacgat catggaagac taccgaaacc
      421 atcctcgcgt caacagctac ctgctttact caaagggccc cgatcggatt ctgggttatt
      481 tcaattttta tggtccatac ggaaagggca ccgatggcaa agccgtgcca gctgtctatg
      541 atccaaccaa tggcacgatc agttcgggcg acatctgtgt ctggggtccg taggctaacc
      601 cctttttgtg agcgccctca tgggattgct ctgcccataa tagaattcgg gtcaatagtg
      661 acgggattgc cgggaacaat gcctaattgt tggtcccaac caagcgacgg gctggaactt
      721 gcttgaatga gcgggatcaa aggcgacggg cgcgagcgac ttgacgatgc gcacgagccg
      781 ttcgcgccca acccggtctg cctggaacac agtgtaacca cgcccatgta gtatctgagg
      841 attcgaatga gttctcccca aatcgaattg agcggtccgt gggaattgac gcatttcccg
      901 caggaaagtc ttgatccgga ggtcgtgctg ggcggcggga gggggttgtg ctggattgcg
      961 gcggaggtgc cgggcaatgt cgtgctggac ctgcaccgcg ccggacagat cgacgacccc
     1021 tacgtgggaa tgaatccgga acaggtgcgg ccgtttgaat atcacgagtg gtggtatcgc
     1081 accacgttca cggtaaatac gccggcgccc gcgggcggga aggtcaacct ggtcttcgag
     1141 ggcatcgata cgttcgcgga gatctacctg gacggccggc tgctcggccg gaccgaaaac
     1201 atgttcgttc cccatcggtt tccgctggcg ccgacgccca aatccggggc gacgcacacg
     1261 cttcacgtgc ggatcaagag cgaccagtat gaagtggcgc ggcgcgactt caacggcgtc
     1321 gtcgaactga tcggcaacga tccgagccgc gtctggattc gcaaggcggc ctacacgcat
     1381 ggctgggaca tttttccgcg gctgctgtcg gccggcatct ggaagcgtgt ctgcctcgaa
     1441 taccagcccg cgtatttcat tgacgacgtc tttgtgttca cgcggcggtt cgaccgggcc
     1501 gccagctcga tcatggcgcg cgtttacatc cgcagcccgc gagcctaccg caccgatctc
     1561 gccgtgcgcc ttcgcgtgcg cgaagcgggg cgggtggtcc atgaagcaac tcacgccgtg
     1621 caggaaacga tggtctgttt cgcgttcgag ctggaacgcc cgcgattgtg gtggccgcgg
     1681 ccgtacggcg agccgtttct ctatcaggtg cgggccgaac tgctggcggg cgatgaggtg
     1741 atcgacggct gggacgggca gttcggcgtt cgttcggtcg agatcgccca ggagccgctc
     1801 gcggatgggc gccaatcgtt ctatttcaag atcaatgacg aaccgatctc gatttacggc
     1861 accaatcatg ttccgctgga ctcgtgcccg tcgcgttatg cgcaacggct gcaaccggcg
     1921 ctggaactgc tcgatgacag ccgctgcaac atggttcggg tctggggtgg cggcgtctat
     1981 gaaacgccgg agttctacgc tgcgtgcgcc cgcatgggcg tcatggtctg gcaggatttc
     2041 atgttcgcct gcgcgctcta tccgcaggag gaggcgtttc tggcgcaggt gcacgaggag
     2101 gcgcgccatg ccattcgcag cctgcgcaac catccctcgg tcgtgctcta ctgcggcgat
     2161 aatgagatcg agctgcatct gagtcaatac ttcgggccgc accagttggg ggcggggaac
     2221 cgccagattg ccaatgccct gttgcccgaa ctggtgatgc gtcacgacgg gacgcggccg
     2281 tatattcctt cgtcgccgtt tacacccatc gccggcgaac cgccgtattc agtcaagggg
     2341 ggcgacacgc atatctggtg ccacggaacg tatttccggg ccccggtcta cagcgaggac
     2401 ctctcgccgt tcatcagcga aatcggccac ctgtcgctgg ccgcgcggga gagcatcgag
     2461 aagatactgc cggctgaggc ggtctggccg cgcgatgagc gattgtggag ctatcgcgcc
     2521 ggctgcctga acgacccggc cttctacacg tatccccacg gctgggtcga ggtgatcgac
     2581 cggaacatca cgaatatgtt cggcgaattg ccagctggcc tggacgatcg gatctttgcc
     2641 tcgcaggtgg ttcaggccga agcctacaag gcgtgggtcg aacgggcgcg gcggcgcaag
     2701 ttcgactgcg gcgggatcct gtggtggaac ctgctcgatg gttgcccgct gatctccaat
     2761 gcggtcgtcg acttctattt caggaagacg atcgcgtatg agtatattcg gcgcgcgcag
     2821 gacgatttgc tgttctgcat cgaaaagatg gacggcgagt tggaactgtt cctggtcagc
     2881 gacagcctgg aggagcgcga ggtcagttac caggtcgtca gtggggacga aatcattgcg
     2941 caagggctcg cgagcgtgcc ggcgaacggg gtggttgcgc tggggagcgc cggcaaggcg
     3001 gcgggcgcgg cgcggctgtt cctgctccgg gcggaagcgg gcgatcgggc gtacttcaac
     3061 cattatctgg acggggagcc gccgttcgat ttggaccggg tgaagggatt ccacgcgcgg
     3121 attgaagccg aagcgcggcg ggcggcgccg accctcgagc ggatcggcgc ggctcgctga
     3181 gcgggccatg gatggagggc gaacacggcc acgcacgggc gcgaatggtg cgcgagacgc
     3241 cgctagcgtg cgatgcgggc gctgccgcct ttgatcagtt tttcgacctc atcgaggctg
     3301 gccatggtcg tgtcgccggg cgtggtcatc gccagggcgc cgtgggcggc gccgtattcg
     3361 accgcctgct ggggcgactt gccggtcatg aagccgtaga tcagtcccga agcgaagctg
     3421 tcgccgccgc cgacgcggtc gaagatctcc aggttcggcc ggtcggtcgc cttgtagaat
     3481 tcgccgtcgc aatacaggac ggcactccag tcgttgacgg tcgcggtttt cgcattgcgc
     3541 agggtggtcg cgacggcctt gaagttgggg tagtccttga cgacgcggcg gatcatctgc
     3601 tggaaggcgg ccgggtccag ctcggagctc ttctcgaggt cggcgccctc gacttcatag
     3661 ccgagggcgg cgctgaagtc ctcctcattg ccgatcatca catcgaccag atgggcgacg
     3721 cgctggttga cctcgcgggc gcgggcctga ccgccgatgt ccttccacag cgaagcgcgg
     3781 tagttcaggt cgtaggagat catggtgccg gcggcgcggg ccgcctccat cgcctcgatc
     3841 atgacgtcgg gggtggtttc ggagaggccg gcgaagatgc cgccggtgtg gaaccagcgg
     3901 gcgccgtcgt gcttgaagac gcgctcccaa tcgacctggc cgggcttcat ttgcgaagcg
     3961 gccgtgtggc cgcggtcgtt ggtgccgcgg gcggcgcgga cgccgaaccc gcgctcggtg
     4021 aagttcagac cgacgcggac gcgccggccg atgccatcaa acggcaccca ctggatataa
     4081 cgggtatcga cgccgccctg caggatcaga tcctcgagca gccggccgat gtcgttgtcg
     4141 gcaatcgcgg tgacgacggc ggtgcgcaag ccgaagcagc ggcgcaggcc gcggatgacg
     4201 ttgtattcgc cgccgccctc ccaggcgtgg aaggtgcgcg cggtgcggac gcggccctcg
     4261 ccggggtcga gccggatcat gatttcaccg agggcgatgg catcccagcg gcaggagtcg
     4321 gcgcctttaa tcgacaacgg ggacatgcag ttctctccca tgtgtggatg atgatgaagg
     4381 ggattctggc gaaaactatt tcagcagcgg ctccagccgg gccacttccg cgggagacag
     4441 ggcgcggttg taggcgcgga cggcgtagat cttgccgttg aagaagctgc gataggccgg
     4501 gtcgacgcgc ccgccgggcg ccgccgcgcc gatgaccgtc acgccgtcgg actcgggggt
     4561 atcgtcgggg gcgttggcga cctcgtcggc gagggcgccg tccacataga gcgcgatcga
     4621 ctcgggattc accaccagcg ccagggtgaa cgggccgccg gcctgctcgg cggtgagcgg
     4681 gtgaatgagc gaatggacct tggttgagcc gcggagctcg caggcgagcc gcggcagatc
     4741 gctcgcgccc tgcaccgaga ggatcagccg cgggggccgg ctccggtcgg taaactcggc
     4801 atagaacggc tgggggattt cgggcatggc cgccagttcg atccgcatgg cgatggtgta
     4861 ttcggacaat ccggcggtcg gggcggcggc aaggaggagg tggtcttcct cggcgccgtc
     4921 aaacacggcc gcgtcgccga cgatctggac gccgtggttg gtgatggcga tccgcggggc
     4981 ctcgacgctc gtattggtgt cggggcgctc ctgcaggcca aggttgaaca gcaggccggc
     5041 gttaagcgcc ttctgttcat cggtcagcgg cgccgcgatg tcgggcgccg gaggggtcag
     5101 cgccggggcc ggcgggttcg ggttcgtggg cgtcggattg gctggggatg gctcggcttg
     5161 ggtttgatcg gttaacggcg cggtggccgc ggaatcggcg gcggccagac cggcggcctt
     5221 gtcgcgcggg cagccggcga cggccagccc ggccagggcg agcagggcgc agagggtgtg
     5281 agagattgag cgcatcggac cggctcggcc ggactccttg ggggcggact cgtacaacga
     5341 gagccctatc atagcccgcc gatccgagaa aggaatggga ttttgcagcg gcgcgcgccg
     5401 gcccgccggg ggcgaaccac cgctgtcgcg ccggggacta ctcggcggtg taggcgcgga
     5461 agcggacggg aaccgtggag ttgaagccgg cgatctttcc cggcgcgtgg acgccctcca
     5521 tggtggtgaa gccgccggcg acgaggaacc cggcgccgga caaggtccca tcgtaaacca
     5581 cgccgttggg cgtgggcgcc cagggatcga gcgtgtagcc cagcgcggcg cgctgaaaag
     5641 cgacgaggta ttgattgtgg tcggagagga cgaacgagcc gagggcgatc acaaattcgt
     5701 ccacgatcag gatgcgccgg accaggggaa agatcaacgg ggggtcccaa tcggcaatga
     5761 cggcgccagt gttgagaccg ttggccaact cgaccgcgga gagaattccg tgattggagt
     5821 tgccgccggt ggagatgaaa tcgcccccca ggtagaggtg aacgccgtcg gaagcaaggc
     5881 aataaacggc cgtggctccg acgttgggac tccaagcctc atcgagcacg gcggtgttga
     5941 cgccgtcggc cggctcgaac gccgcgaggc gattgcgagc agtcccgccc ttgacggtgg
     6001 tgaatggccc accgcagtag acccggttgc cgtggggcag cagggcgcgg cagcgccccc
     6061 cgaaatcggg atgccagggg tcgaccgtga cggcatcgac gccgttggcg gggctgaggg
     6121 ccgcggcgta gttccgcgtg acgccgctga ccgtggtgaa attgccgcag atgtagatct
     6181 tgccaccaga ggacgcgacc tcgtcgattg tgccgttgac atcagggtcc caggcggggt
     6241 cgagcacggc ggggtcggcg gcatgggtgg ggaggttgaa ggcggccatc cggttgcgcg
     6301 cgacgccgtt gacgtcgctg aacgagccgc cgacgtagac cttgtcgccg acgatgtgaa
     6361 tccgatagac gaagccatcg gtgatgttgg ggttccagtc ccacaactgg ccggagtagg
     6421 catcgagggc ggcgatgcgc gcgcggccga caccgtccac gacgctgaat gcgccgccga
     6481 taaggatggt gtcaccaccc tgggcgacgc cgtagatggg gccattgacg ctcgggaaat
     6541 cgaggtcggc cgtggaggtc agggcatgaa gcgacgaagt cgccaggccc aggaataaaa
     6601 tggccaggca agcgaccatt gcgggcgccg aacggcgcga ggaaagcgag cgcatggcgg
     6661 aaccccttgg gtgggatacg gcgcgggagc cggatttaaa gggaaagggc gactatgcaa
     6721 cgagccaagc gcatcgtcaa gtagagaaaa acaaaataat tgttgattta ctgatatgct
     6781 tatcgataac ggagcggttc gggagggggc aaattcttcg agagccgaga accaagacga
     6841 gagccgacgg acgaggttgt aaagaggggg gcggcgattt cagccggcgg cggcggtgaa
     6901 ggcgcgcagc tcgcgcggga cggcgcgcag tcgcatcccc ggggcgacgc ccagttgggc
     6961 gaagcgctcc tgggtgagga cggcgaggac gtcgccgccg tcggcggtca tcaggtgaac
     7021 cttgaccatc gggccggcgg catggatgcg gcggacgatg ccttcgacgc cgaggtcggt
     7081 ctggtcgggg ggcagcagat cgacctggtg gggccgggca aaggcggcgc cggggtaagg
     7141 ggtggagcgt gaatcggggg tgggcagggc gacgagcgcg gcgccggcct cgctgcgctc
     7201 gtggaacagg ttgacgtcgc ccaggaaggt gtaaacgaag ggggtggcgg ggtgatgata
     7261 gacgtcggcg ggggcgccga cctgttcgat gcgcccttca ttcatgatga cgacgcggtc
     7321 ggcgacctcg agggcttcct cctgatcgtg ggtgacgaag atgctcgtca tgtggagctc
     7381 atcgtggagg cggcgcagcc agcggcgcag ttcctggcgg atgcgggcat cgagcgcgcc
     7441 gaagggttcg tcgagcagca ggacgcgcgg ctggatggcg agcgcgcggg cgagggcgac
     7501 gcgctggcgc tggccgccgg agagctggtc ggggcggtgc tgctcgagct tgtcgagctg
     7561 gacgaggcgg aggagttcgc ggacgcggcg ggcgatctcg ttgcgcgggg ggcggtcctt
     7621 gcgcggcagg acacgcagac cgaaggcgac gttttcgaag accgacatgt ggcggaacag
     7681 agcgtagtgc tggaagacga agccgacctt gcgttcgccg acgcgcatgg cggagacgtc
     7741 ctggccgttg acctcgatcg tgccgccgcg gtcggggtcg caggcgtcga ggccggcgat
     7801 gatgcgcagc agggtcgtct tgcccgaccc ggagggaccg agcagcgcga gcaattcgcc
     7861 ctcctcgacg gtcaggttca cggcgtccag cgccttgaac gagccgaacg accgggtgat
     7921 gccggttaac cggatactca tgagtgacct ccccgggggc gcggggcgat gccgcgcgcg
     7981 actttccatt cgagcgccgt cttgaggacg agcgtgacga gcgccagcag aatcaggagc
     8041 gaggcgaccg agaaggcggc cgcgaaatta tattcgttgt agaggacttc gacatgcagc
     8101 ggcagcgtgt tggtgcggcc gcggatgtga ccggagacga cggagacggc gccgaactcg
     8161 cccatcgccc gcgcgttgca caggatcagg ccgtagagca ggccccactt gatgttgggc
     8221 agcgtgacgc gccagaaggt ctgccacgga ccggctccca gcaccagcgc ggcctgttcc
     8281 tgctcgctgc cctgttcctg catcagcggg atcagttcgc gggcgacgaa cgggaacgtg
     8341 acgaacacgg tggcgagcag gatgcccggc acggcgaaga tgatgcggat ctcgtgcgcg
     8401 gcgagccact ggccgagcca gccgtgcagg ccgaagagca ggatgaagac aaggccggag
     8461 atgacggggg agacggcgaa ggggagatcg atgagcgaga gcaggaggct cttgccgcgg
     8521 aactcgaagc gggcgatggc ccaggccgcc gccaggccga agaggccgtt ggccagcagc
     8581 gcgactccgg ccgtgagcag ggtgaggcgg atcgcggcga gagcggccgg ttcggtgatg
     8641 gcgtgcaggc aggtggcgag gcccatgcgc aacgcctcga cgagcacgac cgccagcggc
     8701 aggacgagca gcagggcgag gaacgcgagc gtgacggcga tcagcgtggc gcggaccggc
     8761 cgaaaccggg cgcgggcgag gccgggatgg tgggagacgg cgatgctcat gcggcgcctc
     8821 cctgacccag tcggcgggcg ctccagtgct gcagcgcgtt gatgccgagc atcagggcga
     8881 acgaggcgag cagcatgacg acggcgatgg cggtcgcgcc ggcgtaatcg tattcctcca
     8941 gccgcgtgac gatgagcagc gaggcaattt cggtgcggta ggggagattg cccgagatga
     9001 agatgaccga gccgtattcg cccaggccgc gcgcgaaggc cagggcgaag ccggtcagca
     9061 gcgcgggcgc gatgaccggg aacaggaccc ggcgcagcgt ctgccacggg ttggcgccga
     9121 ggctgacggc ggcttcctcc tgctcgcgtt cgagcccttc gatgaccggc tgcagcgtgc
     9181 ggacgacgaa cggcaggccg atgaacgtca gcgcgatcgt gatgcccagc ggcgagtagg
     9241 cggcgcggat gccgagcggc tccagccagc ggccaaccca gccgtggctg gagtagagcg
     9301 tggtgagcgc gatgccggag acggcagtgg gcagggcgaa ggggagatcg atgagcgcat
     9361 cggcaaggcg ccggccgggg aagcggtagc gcaccaggct ccaggcgacg atcaggccga
     9421 agagggcgtt gacggcggcg gcgacgaacg ccgtggtgaa cgtgaggcgg taggaggcga
     9481 ggacgcgcgg gttgaccgtc gcccgccaga actcctccca gcccagcgtg ccggccttga
     9541 aaaagagcgc cgagaggggc agcagcacga tgaggctgag gtagaagagg gtgaagccga
     9601 gcgagaggcc gaaaccgggc agcgcggcgg gggcggcgga gcggccaagc aggcgcggga
     9661 ggagcggtgc ggcggcggcg gacattcaag gcctccgctg gatctgatcg aagatggcgc
     9721 cgtcggcgaa gtgttcggcg tgggctcggc tccagccgcc gaaaacgtcg tcgatggtga
     9781 acatttccag ctcggggaac cgcgccaggt cctgggggtc ggctttctcg gaaagaacgg
     9841 gccggtagaa gtggcgcgcg gcgagccgct ggccgacgtc gctgtagagg aactccaggt
     9901 aggcgcgggc cgcgtcggtc gtgccgtggc gttcggtatt ggcgtcgatc caggcgacgg
     9961 ggggctcggc cagaatgctg atcgaaggga cgacgatctc gtagtcgtcg cggccgaact
    10021 gttcgagcgc gagaaaggct tcgttctccc aggcgagcag gacgtcgccc agccggcgct
    10081 ggacgaacgt attcgtggcg ccgcgggcgc cggtgtcgag gacggggacg tgggtgaaca
    10141 gctcggcaac gaactggcgc gccttggcct gggcctgctc gacttcggcg gcggcggcgg
    10201 ggtcatgcag ccggtttaga tcgcccagct cgcggcggag ggcgaaaccc caggccgcca
    10261 ggtagttcca gcaagcaccg ccggaggttt tcgggttggg cgtgatgact tcgattcccg
    10321 gctgaatcag gtcgtcccaa tcggcgatgc cgcgggggtt gcccttgcgc accaggaaga
    10381 cgatcgtcga ggtgtagggg cagctctggt gggggagccg gctctgccag tcggcgggga
    10441 ggaggccgga gcgtttggcg atggagtcga tgtcgtgggc cagggccagc gtcacgacat
    10501 cggcctcgag gccgtcgatg accgagcggg cctgtttgcc ggagccgccg tgggactgct
    10561 ggacggcgat cgcgccgccg tgctcggccg cccagtgttc gccgaaggcg gcattaaagg
    10621 cttcgtagaa ctcgcgcgtg ggatcatagg agacattgag cagttccagg gcgtcggccg
    10681 ccggggcgag gggagcggtg agcgccgcca ggaacgagag gagcaggcta acggccgccg
    10741 cccgcaggcg gaggcggggg gctgcgccgg gacggggcgc gaggcgagag gcgatggggt
    10801 cggcaacggg ataagggttc atggataatc ctcgcgggga gagtggtgtg gaggggggag
    10861 ggggtctggt cggaaaagaa gtcccggccc cactcggcgc agggcctagg cgaggaccgg
    10921 gcc
//
LOCUS       DAOPCO010000131        10870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_5904_length_10870_cov_5.850301, whole genome shotgun sequence.
ACCESSION   DAOPCO010000131 DAOPCO010000000
VERSION     DAOPCO010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_10870_cov_5.850301"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLS90_10105"
     CDS             <1..287
                     /locus_tag="PLS90_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPK02794.1"
                     /translation="TAARRQDAVGLTLTAYHHISFDRDHVMPEADLRRLLTDQGFTVA
                     NMSYRVTDDGKVFEYRMIIRTTNPSDTARLAAALRELKSVREFRISPTGD"
     gene            complement(407..661)
                     /locus_tag="PLS90_10110"
     CDS             complement(407..661)
                     /locus_tag="PLS90_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02795.1"
                     /translation="MTESKPRAETQERYKDFLTLFSDGDLIMLPAFQLDNYLERLLDK
                     DTGTDAFLNYRAFLMLEEERLALPPELLEPADEPRSDQAL"
     gene            complement(872..3553)
                     /locus_tag="PLS90_10115"
     CDS             complement(872..3553)
                     /locus_tag="PLS90_10115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPK02796.1"
                     /translation="MLEKTRQSLESALRYNPSNGELMASLAEVYVRLGMFDERTMELV
                     EAVLSQQGENPLLQQAQSIGLLIEQTRTLQENLAEGQPPPDPSTIQHSLDNLSTYLKN
                     QPECESAWVAWTRLQLIKGDLVRARKGIERLVRLEATDLARSLGPALLWRQRQGASGE
                     TGCHLARIYLTLGETLQAMELLEKLYDDGFAEAGPLLFEIYQRQFSPNQPEKVPETHR
                     QRFFMLLLDHADAERTNQWLRKASLLGWQVGVFSQSYAETLIEHGELDEAFLVLQRTI
                     MDDYVRQLLNQIALRYEEQDDVDKAVAVLRYINDNSLVEQDVQQNRERDLVRSSEISM
                     AELHLKNGRPEDALVHYINALCNCPAPEPDLLRTIEELFDAAAPHEAEPLLRLGLYFR
                     NLHDYPKAIFWLNQALEIDGENREVIAELESLYDDLLQANPDLPQLRLDLGRLYYHTR
                     RWSKAIDELRVAAGWAEQADAANRLLARAYHRDGQINKALDKFKLVTPDEDDCAELFK
                     LYEEFMDQKAWRDAQLSLELIAETHPQFRDVTMRLHQLRARQQPQWGGSAEPAGDPRM
                     RELIGDLAVGRYNYMERLGSGGMGVVHKVFDVRNNRVVAMKILRDSLSSSSKALDRFF
                     REARIAASLSHTNIVNIYDYNISNMSGQSYIVMEYVDGPSLRELVDRQFAETIETPLD
                     YITEILHYSVQLCDALQASHANGVIHRDIKPDNIMITQEGVVKITDFGIVHIEEATFT
                     PSGAMLGTPRYMSPEQVTGGRVDARSDIYSVGILLYEVLTGTPPFFTGDIAFQQVHKD
                     PSNPRELNPKLPESLAEGILRCLMKKPDDRFQDAMELKASLGRILERMGGCAKYSNRT
                     EVVEDGAPFLRAAPIASGAGAAPEGPAVGSDAELDLE"
     gene            3977..4753
                     /locus_tag="PLS90_10120"
     CDS             3977..4753
                     /locus_tag="PLS90_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HPK02797.1"
                     /translation="MDLNGKSGVILGVANKYSIAWAIAEAARAAGARLAFNYQNERLA
                     QSVKELVNDGCPGSICLPCDLGDDDQIDAFFAEVGHQFGGKLDFLVHSVAFANREDLA
                     GRFVDTSRNGFNLALEVSAYTLVAAARRAAPLMEAAGGGAIITMTYFGAEKVIKNYNV
                     MGVAKAALEASVRYLAADLGAQAIRVNAISAGPVKTLAARGISGFSSMLTIAAERAPL
                     KRNVDPREIAQTAIFLASDGGSGITGETLYVDGGYNIIGL"
     gene            4850..6301
                     /locus_tag="PLS90_10125"
     CDS             4850..6301
                     /locus_tag="PLS90_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02798.1"
                     /translation="MSPLLALVLYGLVLIGFGVWYRQSARDGTGFFLAGRALSAAEVG
                     LSLTATGFGGSAILFASGLAWARGLPALWFTGSLGLGLAILGATLAGRVRAGGDHSLA
                     GLAGRLYGDGVRWLASLLLVVVEVAFFALTLKSISLLTGTLLGPEHWLLTHQAVYEGG
                     VCLVIVLYVLLGGHKAVATTDILQLGLVALVLIGILLPVALLRADWAALPAAAWRFPF
                     SAGDPAAGLTPTGPLFAVNMFILMGLSGIVGGDVYSKILSARDERAARRGAFIGAAGA
                     ALLALCVALLALAGRSLLTPQVLAGRSPQLAIPLLSQALLHPVLFQLMVLGLLSALLS
                     TADSVLITGATVLALDVLRTGERVGAARLRIVTLALALAGLALALWFETLLNLIQFAY
                     TLLTASLVPPILAGLALPARRRPSRGGAAAAMVLGLAAAGAWRVAAPRLPEAWRLVEP
                     ATVGVAVNVAVLLAGIMLQRWARQGPSRQTTAA"
     gene            complement(6246..7439)
                     /locus_tag="PLS90_10130"
     CDS             complement(6246..7439)
                     /locus_tag="PLS90_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02799.1"
                     /translation="MPAPALLQVCAVDFTAWHFLRPLMRAQLEAGFDVHLACRPGAYL
                     DALGQEGFTVHPIPFERSYDLLAHWRAARALRKLLRREPFTVVHAHTPIASLIARRVA
                     RRCRVPVVLYTAHGFYFHDAMAPRARRFHIALERWGQRHADFLFTQSREDLETAVREG
                     IAPAARARAIGNGVDIDRFRQWRFSAEERAATRAELGLAPDAGPIVMMIGRLVREKGY
                     FEFLEAFARAREELPAARAVLIGEALASDHDASAAEIFARAEALGLKDSVIFTGARRD
                     IPRLLAIADVYCLPSWREGMPRSIIEAMAMTLPVVTTDIRGCREEVVDGETGRIVPAR
                     DSAALAGALLELLRDEALRRQMGAAARRRAVELYDERKVIAAQMEVMQRLFAEKGLAW
                     PTAEA"
     gene            complement(7454..9382)
                     /locus_tag="PLS90_10135"
     CDS             complement(7454..9382)
                     /locus_tag="PLS90_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02800.1"
                     /translation="MRCSGPGVGRGALALALVACLAAGGCARAGRAVSPLVAAAPPRI
                     EHFDVDKVTYLSLSPEVECRLTLGGAPPPGLAAEFRIHDLARGVVLASAAVPLDGVST
                     RTVAASLPVRVSQMTGLLAEARLIEPRGGVAVDAAECPFDVTDDLRTNFRYAGETYGG
                     VSRALEPRPIGEGTPDDFWKGIPVGTPPQVQLDRLRACFQNVAEVSVEEGRGHYTCWE
                     EGSRQFWNKSAYHTMDIWEFSHERILHLYREGDRRGIYMLPWTDGKGARKEYFDEGFP
                     WIDPWNPNGYYPGNWLMNLDPKFHRPLTPEELKLAHIDLHGTESWIDFVVEELVRMTD
                     HYRPRIIWWDNSYGGEHIEYTLSKLRARWANEQPERTPPLLMINGNAGYHSDIYWIEH
                     DLPARMDGYLWLMRQLIKDRPAVAEGSATIFSGQHHPAAASGQQAGISDPKNIYNDAA
                     TTFMFRQLLFECLASDPRIVGQQQWPRNALEWRIGQFPEAFADHARTWGFQTLFAHVY
                     NARDVRPYALAASVEALGAPATREDAVEDGKLHLVARAPLSDPDRIYLHVFNYLGTTI
                     DVLARRPRPARADVTLRLDVPGFAGEYRVRAFSPDFARYGDCVAPATVAEANHLQFDV
                     PVETYTLVIIDRPRAGWR"
     gene            complement(9520..9969)
                     /locus_tag="PLS90_10140"
     CDS             complement(9520..9969)
                     /locus_tag="PLS90_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02801.1"
                     /translation="MLRSDDGRRLERLDFCPECWQVMKNEAYESFWITRRERKDKAIP
                     RLSKRERATALRVLFESLWDRREEEDLAAHLFFLSHLLMKWGGLKWRENSSDIHGNEV
                     VVFEDPASGDLLEIGAAPLDDDRLLAIKEEVEGFLRQYAAEGQEISL"
     gene            10402..>10870
                     /locus_tag="PLS90_10145"
     CDS             10402..>10870
                     /locus_tag="PLS90_10145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK02802.1"
                     /translation="MAHAAHLLDADIPLARLPGHWVLARLGKRVLRPGGLDSTRRLLK
                     ALAIGPADRVVEFAPGLGLTARRILGLRPAAYTAVERDESAARRLRDCLTGPAIQIVN
                     AGAEASGLPDQCAEVVLGEAMLTMQPPEKKAAIVREAARLLRPGGRYGLHEIAL"
BASE COUNT         1649 a         3867 c         3470 g         1884 t
ORIGIN      
        1 ccaccgccgc ccgccgacaa gatgcggttg gtttgacgct tactgcgtat catcatattt
       61 cctttgatcg cgatcacgtc atgcccgagg ccgacctgcg ccgcctgctg accgatcagg
      121 gttttaccgt ggcgaatatg agctatcgcg tcaccgatga cggcaaggtt ttcgagtacc
      181 gcatgatcat ccgcacgacc aatcccagcg acaccgcgcg tctggccgcc gcgctgcgtg
      241 aactgaaatc cgtgcgtgaa ttccgcattt cgcccaccgg cgactgacgc aggcaacgca
      301 gctctgcgaa tgcatgtcag gcaagatgcc tgtcccgcaa acatgcacga aaacgccccg
      361 atctggccag accggggcgc gggaaaggcg gggtggaccg gggtggttac agcgcctggt
      421 cggagcgggg ctcgtcggct ggctccagca gttccggcgg cagcgccagc cgctcttcct
      481 ccagcatcag aaacgcgcgg tagttcagga acgcgtcggt tccggtatcc ttgtccagca
      541 ggcgttccag atagttgtcc agttgaaagg ccggcagcat gatcaagtcg ccgtcggaga
      601 agagtgtcaa gaaatcctta tatcgctcct gagtttcggc cctcggcttc gattcggtca
      661 tgaagtgctc tccttgcggt atccctcgac atcgagaggg agcaagggcc atgcctctgt
      721 ccgaaatcga ccccgtttgc gcataaaatg agcaatatca atgattcatt gccgatacga
      781 cggatagccc ccgccagcct gcgcgccgcg cgttatgcgc ttcttcgcac acttccagtc
      841 gcaatttgag aatcggcgcc atcccgcgcc actactccag atccagctcg gcatcgctgc
      901 cgaccgccgg gccctcggga gcggccccgg cgcccgaagc aatgggcgcg gcccggagga
      961 acggcgcgcc gtcctcgacc acctcggtcc ggttggagta tttcgcgcag ccgcccatcc
     1021 gctccagaat ccgccccagc gacgccttca gctccatcgc gtcctggaac cggtcgtcgg
     1081 gcttcttcat caggcaacgc aggatgcctt cggccagcga ctcggggagc ttggggttga
     1141 gctcacgcgg gttggagggg tccttgtgca cctgctggaa cgcgatgtcg ccggtaaaga
     1201 agggcggcgt gccggtcagc acttcataga gcagaatccc gaccgaataa atgtcgctgc
     1261 gcgcgtcgac ccggccgccg gtcacctgtt ccggcgacat gtagcgcggc gtgccgagca
     1321 tcgcccccga aggggtgaac gtcgcctcct cgatgtggac gatgccaaag tcggtgatct
     1381 taaccacgcc ctcctgcgtg atcatgatgt tgtcgggttt gatatcgcgg tggatcacgc
     1441 cgttggcgtg cgaggcttgc agcgcgtcgc agagctgaac cgagtagtgc aggatctcgg
     1501 tgatgtaatc caggggcgtt tcgatcgtct cggcaaactg tcggtcgacc agctcgcgca
     1561 gcgacggccc atcgacatac tccatcacga tgtagctctg cccgctcatg ttgctgatgt
     1621 tgtagtcgta gatgttgacg atgttggtgt ggctgagcga ggcggcgatg cgcgcttcgc
     1681 ggaagaagcg atcgagcgcc ttggaactgc tcgagagcga atcgcgcagg attttcatcg
     1741 ccacgacccg gttgttgcgc acgtcgaaca ccttgtgcac gacgcccatc ccgcccgacc
     1801 cgagccgttc catgtaatta tagcggccga cggccaggtc gccgatcagt tcgcgcatgc
     1861 gcggatcgcc ggccggttcg gcgctgcccc cccactgcgg ctgctggcgc gcgcgaagct
     1921 ggtgcagccg catcgtcacg tcgcggaact gcgggtgagt ctcggcgatc agttccagcg
     1981 acagttgcgc gtcgcgccac gccttctggt ccatgaactc ctcgtagagc ttgaacagtt
     2041 ccgcgcaatc gtcctcgtcg ggcgtcacca gcttgaactt gtcgagcgcc ttgttgatct
     2101 ggccgtcgcg atggtaggcg cgcgccagca ggcgattggc cgcatcggcc tgttcggccc
     2161 agccggccgc cacgcgcagt tcgtcgatcg ccttgctcca acgccgggtg tgatagtaga
     2221 gccggcccag atccagccgt agctgcggca ggtcgggatt ggcttgcagc aggtcgtcgt
     2281 agagcgactc cagttccgcg atcacctcgc ggttctctcc gtcgatctcc agcgcctgat
     2341 tcagccagaa aatcgccttg ggataatcat ggagattgcg gaagtagagg cccaggcgca
     2401 gcagcggttc ggcctcgtgc ggcgcggcgg cgtcgaacag ctcctcgatc gtccgcagca
     2461 ggtccggctc gggcgccggg cagttgcaca acgcgttgat gtagtgcacc agcgcgtcct
     2521 cggggcggcc gtttttcagg tggagctccg ccatcgagat ctccgagctg cgcaccaggt
     2581 cccgctcgcg gttctgctgc acatcctgct ccaccaggct gttgtcgttg atgtagcgca
     2641 gaacggcaac cgccttgtcc acgtcgtcct gttcctcgta gcgcagcgcg atctggttga
     2701 gcaactggcg aacataatcg tccatgatcg tgcgctgcag gaccaggaac gcctcgtcca
     2761 gttcgccgtg ctcgatcaac gtctcggcat aggactgcga gaagacgccc acctgccagc
     2821 ccagcagcga ggccttgcgc agccactggt tggtgcgctc ggcatcggca tggtccagca
     2881 gcagcatgaa gaaccgctgc cggtgggtct cgggcacctt ttccggctga ttggggctga
     2941 actggcgctg atagatctcg aacaggagcg ggccggcttc ggcgaaaccg tcgtcataca
     3001 gcttctcgag cagttccatc gcctggagcg tctcgcccag cgtcaggtaa atccgcgcca
     3061 ggtggcagcc agtctcgccc gaggccccct gccgctggcg ccagagcagc gccgggccca
     3121 ggctgcgcgc cagatcggtc gcctccagcc ggaccagccg ctcgatcccc ttgcgcgccc
     3181 gtaccaagtc gcccttgatc agttgcagcc gcgtccacgc cacccaggcc gattcgcact
     3241 cgggctgatt cttcaggtag gtcgacagat tgtcgagcga atgctggatc gtcgacggat
     3301 cgggcggcgg ctggccctcg gccagattct cctgcaacgt ccgggtctgc tcgatcagca
     3361 ggccgatcga ctgcgcctgt tgcagcagcg ggttttcccc ctgctggctc agcaccgcct
     3421 cgaccagttc catcgtccgc tcatcgaaca taccgagccg gacgtagact tcggccagcg
     3481 acgccatcaa ttccccattg ctgggattgt agcgcagcgc gctctcgagc gactgccggg
     3541 ttttttcgag cattgacttc atatcggccc acgagtcggc ggcgacgcgc cgggcggccg
     3601 gcacgccgga gggatggggt gcgccttggc tccctgttcc gtatcggcgt ttgccaccct
     3661 ccggttaact ccaatctgtc gccgccgaca cgggcgccgg accgagcgtt tgggatgctc
     3721 ccctcccgta tcgcttatgt ctcgtgtatt cttttgcgtc ccttacgccc ttgccgcggt
     3781 tcttcgcaag aacgaccgat cacgagacca aggacgcggg gcgagggacg aggcaccttt
     3841 tcgcttcaca ggtgcccggg gcgccgataa aatcggcaac ccgtcgtgtg tgcaggtccg
     3901 cgttgactcc cgtggccggt acgggcctgg catcggatgg ccgcattctc acatccgcgc
     3961 agaaagaatc agtcccatgg atctcaacgg caaaagcggc gtcatcctgg gggtggccaa
     4021 caaatactcc atcgcctggg cgatcgcgga agccgcccgc gccgccggcg cccgcctcgc
     4081 cttcaactat cagaacgagc gcctggcgca gagcgtcaag gagctggtca acgacggctg
     4141 cccgggctca atctgtctgc cttgcgacct gggcgacgat gatcagatcg atgccttctt
     4201 cgcggaagtc ggccaccagt tcggcggcaa actcgatttt ctggtccact cggtcgcctt
     4261 cgccaaccgc gaggacctgg ccggccgctt cgtcgatacc tcgcgcaacg gcttcaatct
     4321 ggcgctcgag gtcagcgcct acacattggt ggcggcggcc cggcgcgccg ccccgctgat
     4381 ggaagccgcg ggcggcggcg cgatcatcac gatgacttac ttcggcgccg aaaaagtcat
     4441 caaaaactac aacgtcatgg gcgtcgccaa ggccgcgctg gaagcctcgg tgcgctatct
     4501 ggcggccgat ctgggcgccc aggcgatccg cgtcaacgct atttcggccg ggccggtcaa
     4561 gaccctcgcc gcccgcggca tctccgggtt ctcctcgatg ctgacgatcg cggccgagcg
     4621 cgccccgctc aaacgcaatg tcgacccccg cgagatcgcc cagaccgcca tcttcctggc
     4681 ttccgacggc ggcagcggca tcaccggcga aaccctctac gtcgatggcg ggtacaacat
     4741 catcggactc tgacggctcg cggctcgcgt cccttgtccc ttgccctcaa gatccgatga
     4801 tcgatgattt gcgcatggat gcgtccgcaa ttcggcgaag cttggaggag tgtctcccct
     4861 gctcgcattg gtgctctacg gcctggtgct gatcggcttc ggcgtctggt accgccagtc
     4921 ggcgcgcgac ggcacggggt tcttccttgc cggccgggcg ctcagtgccg ccgaagtcgg
     4981 actctcgctg accgccaccg gcttcggcgg ctccgcgatc ctgtttgcct ccggtctggc
     5041 ctgggcgcgc ggcctgcccg cgctgtggtt taccggctcg ctcggcctgg ggctggcgat
     5101 cctgggcgcg acgctggccg gccgcgtccg cgccggcggc gaccactcgc tggcgggcct
     5161 ggccggccgc ctctacggcg acggcgtccg ctggctcgcc tcgctcctgc tggtcgtggt
     5221 cgaagtcgcc tttttcgcgc tcacgctcaa aagcatttcg ctcctgaccg gcacattgct
     5281 cgggccggag cactggctgc tcacccatca ggcggtctac gagggcggcg tctgcctggt
     5341 catcgtgctc tacgtcctgc tgggcggaca caaggccgtc gccaccaccg acatcctgca
     5401 gctcgggctg gtcgcgctgg tcctgatcgg gatcctgctc ccggtggccc tgctgcgcgc
     5461 cgactgggcg gccctgcccg ccgcggcgtg gcgctttccc ttttccgccg gcgatccggc
     5521 cgccggcctg acccccaccg gcccgctctt cgccgtcaac atgttcatcc tgatgggcct
     5581 ttcgggcatc gtggggggcg atgtgtactc caaaattctg agcgcgcgcg atgaacgggc
     5641 cgcccgccgg ggagcattca tcggcgccgc cggcgctgcc ctgctcgccc tctgtgtcgc
     5701 gctgctggcg ctggccggcc ggtcgctcct gacgccgcag gttctggccg gccgctcccc
     5761 ccagctcgcc atcccgctcc tgtcgcaggc actgctgcac cccgtcctct tccagctcat
     5821 ggtgctggga ttgctctcgg cgctgctctc gaccgccgat tcggtgctca tcaccggcgc
     5881 gaccgtgctg gcgctggacg tgctgcgcac cggcgaacgg gtcggcgcgg cccgcctgcg
     5941 catcgtgacg ctggccctcg cgctggccgg cctcgcgctg gccctgtggt tcgagacgct
     6001 gctcaacctg atccagttcg cctacacact gctgacggcg tcgctcgtgc cgccgatcct
     6061 ggcggggctg gcgttgccgg cgcggcggcg tccgtcgcgc ggcggcgccg ccgccgcgat
     6121 ggtcctcgga ctggcggccg ccggcgcctg gcgggtcgcc gcgccgcgcc tgcccgaagc
     6181 ctggcggctg gtcgagccgg cgacggtcgg cgtcgccgtt aatgtcgccg tgctgctggc
     6241 cggcattatg cttcagcggt gggccaggca aggcccttct cggcaaacaa ccgctgcatg
     6301 acttccatct gtgcggcgat caccttgcgt tcgtcgtaga gctccaccgc gcggcggcgc
     6361 gcggcggctc ccatttggcg ccgcagcgct tcgtcgcgca gcagttcgag caacgccccg
     6421 gccagcgccg cgctgtcgcg cgccggcacg atccgccccg tttcgccgtc gaccacctct
     6481 tcccggcagc cgcggatgtc ggtcgtcacg accggcagcg tcatcgccat cgcctcgatg
     6541 atcgagcgcg gcattccctc gcgccacgac gggaggcagt agacatcggc gatcgccagc
     6601 aggcgcggga tgtcgcgccg cgcgccggtg aaaatgaccg agtccttcag cccgagcgcc
     6661 tcggcgcgcg cgaaaatctc cgccgccgac gcgtcgtgat cgctcgccag tgcttcgccg
     6721 atcagcaccg cccgcgccgc cggcagttcc tcccgcgcgc gcgcgaacgc ctccagaaac
     6781 tcaaagtagc ccttctcccg cacgagccgg ccgatcatca tcacgatcgg cccggcgtcg
     6841 ggcgccagcc ccagctcggc gcgcgtcgcc gcccgctcct ccgcgctgaa gcgccactgc
     6901 cggaagcggt cgatgtcgac gccgttgccg atcgcgcgcg cgcgcgcggc cggggcgatc
     6961 ccctcgcgca cggccgtctc gaggtcctcg cggctctggg tgaacaggaa atcggcgtgg
     7021 cgctggcccc agcgttccag ggcgatgtgg aaccgccggg cgcgcggcgc catcgcgtcg
     7081 tgaaagtaga acccgtgcgc cgtgtacagc acgaccggca cgcggcagcg ccgggcgacg
     7141 cgccgcgcga tgagcgacgc gatcggcgtg tgcgcgtgca ccaccgtgaa cggctcgcgc
     7201 cgcagcagct tgcgcaaggc gcgcgccgcc cgccagtgcg ccagcagatc gtagctgcgc
     7261 tcgaacggaa tcgggtgcac cgtgaagcct tcctgaccca gcgcatccag ataggcgccg
     7321 ggccggcacg ccaggtgcac gtcgaacccc gcttccagtt gcgcgcgcat cagcggccgc
     7381 aggaagtgcc acgcggtgaa atccacggcg cagacctgga gcagggcggg ggcgggcatc
     7441 gggcgcgaac ccctcagcgc caaccggcgc gcggccggtc gatgatcacc agcgtgtagg
     7501 tttcgacggg cacgtcgaat tggagatggt ttgcctcggc gaccgtcgcg ggcgcgacgc
     7561 aatcgccgta gcgcgcgaaa tcgggcgaga acgcgcgcac gcggtattcc ccggcgaagc
     7621 ccggcacgtc gagccgcagc gttacgtccg cgcgcgccgg ccgcggccgc cgcgccagca
     7681 cgtcgatcgt cgtccccagg tagttgaaca cgtgcaggta gatccggtcg ggatccgaca
     7741 gcggcgcgcg cgccaccagg tgcagtttgc cgtcctcgac cgcatcctcg cgcgtcgccg
     7801 gcgcgccgag cgcctcgacc gacgccgcca gggcgtaggg ccgcacgtcg cgcgcgttgt
     7861 acacatgggc gaacagcgtc tggaaccccc acgtgcgcgc gtgatcggcg aacgcctcgg
     7921 ggaactggcc gatccgccac tccagcgcgt tgcgcggcca ttgctgctgg ccgacgatgc
     7981 gcggatcgct ggcgaggcac tcgaagagca actgccggaa catgaacgtc gtcgcggcgt
     8041 cgttgtaaat gttcttcgga tcgctgatcc ccgcctgctg gcccgacgcc gccgccgggt
     8101 ggtgctgccc cgagaagatc gtcgcgctcc cctccgcgac cgccggccgg tccttgatca
     8161 gctggcgcat cagccacagg taaccgtcca tccgcgccgg caggtcgtgc tcgatccagt
     8221 aaatgtcgct gtggtaaccg gcgttgccgt tgatcatcag cagcggcggc gtccgctccg
     8281 gctgctcgtt cgcccagcgg gcgcgcagct tgctcagcgt gtactcgatg tgctcgccgc
     8341 cgtacgagtt atcccaccag atgatgcgcg gccggtagtg gtcggtcatg cgcaccagtt
     8401 cctcgacgac aaagtcgatc cagctctccg tcccgtgcag atcgatgtgc gccagcttca
     8461 gctcctccgg cgtcaacggc cggtggaact tcgggtccag gttcatcagc cagttgccgg
     8521 ggtagtagcc gttgggattc cacgggtcga tccagggaaa accctcgtca aagtattcct
     8581 tgcgcgcccc cttgccgtcg gtccacggca gcatgtagat tccgcgccgg tcgccctcgc
     8641 gatacagatg caggatccgc tcgtggctga actcccagat gtccatcgtg tggtaggccg
     8701 acttgttcca gaactggcgg ctgccctcct cccagcaggt gtagtggccg cgcccctcct
     8761 cgacggagac ctcggccacg ttctggaagc aggcgcgcag ccggtcgagc tgcacctgcg
     8821 ggggcgtgcc gacggggatg cctttccaga aatcgtcggg ggtgccctcg ccgatcggcc
     8881 gcggttcgag ggcccgcgac acgccgccgt atgtctcccc cgcgtaacgg aaattcgtgc
     8941 gcaggtcgtc ggtcacgtcg aacgggcatt ccgcggcatc gaccgccacc ccgccgcgcg
     9001 gctcgatcag ccgcgcctcg gccagcagcc ccgtcatttg cgatacgcga accggcagcg
     9061 aggcggcgac ggtgcgcgtg ctcaccccgt cgagcgggac cgccgccgat gccagcacca
     9121 ccccgcgggc cagatcatga atccggaact cggcggccag ccccggcggc ggcgcgccgc
     9181 cgagcgtcaa ccggcactcc acctcgggcg acaagctcag gtaagtcacc ttgtccacgt
     9241 caaaatgctc gatgcgcggc ggcgccgccg cgactagcgg gctcacggcg cgacccgctc
     9301 gcgcgcagcc gccggccgcc aggcaggcga ccagcgccaa cgccagcgcg ccccttccca
     9361 ccccaggccc ggagcagcgc atgagcgggc tcctttcagc atgtcgattg atttctggcg
     9421 cgacgaacga gggaccatgg cggcggctcc cccgccgggc cggtgcgaac ggtcaaaccg
     9481 cggggttcga tgtcgaggac gagggacgag cgacggccat cagagcgaaa tttcctgccc
     9541 ctcggcggca tactggcgca ggaacccctc gacctcctcc ttgatcgcca gcaggcggtc
     9601 gtcgtcgagc ggcgccgcgc cgatctccag caaatcgccc gatgccggat cctcgaagac
     9661 caccacctcg ttgccgtgga tgtcgctcga gttctcccgc catttcagcc cgccccattt
     9721 catcagcaga tgcgacagga aaaacaggtg ggccgccagg tcctcctcct cgcgccggtc
     9781 ccacagcgac tcgaacagga cgcgcagcgc cgtcgcccgc tcgcgcttgc tcaaccgcgg
     9841 gatcgccttg tccttccgct cgcgacgcgt gatccagaac gattcatacg cctcgttctt
     9901 catgacctgc cagcactcgg ggcagaagtc gagccgttcg agccggcgcc cgtcgtccga
     9961 gcgcagcatg ctgggatgac gctagatcac gtcgagcgcc tcgccacagt tcaggcacgt
    10021 cttgttcgga cggctcaggg tcaacagggc ttccttatcc atcggtcggc gacacctgcg
    10081 gcaacgggaa ctggagcaac cggctgcccg cccggcggcg ggggcccaac gccacgccgc
    10141 ccagtgtagt gacaaaccgc atccagactc aagcaaggaa gcgccacgct gcatcgagga
    10201 ccaaggtcgg agccggcttt tggatcgctg tgactgagtg ttgcgctcaa agccccgctt
    10261 gtcctggttc cgtttggcct ctcgagattt ttcgcctaca ggatctaaat accttgacat
    10321 catcatatcg ttgcctatag taaacttcag gtgcacggcg gaaaccggcc ggcgcctccc
    10381 cccccgaaag gagcccccat catggctcat gccgcccatc tgctcgacgc cgatatcccc
    10441 ctggcccggc tccccggcca ctgggtgctg gcgcgcctcg gcaaacgcgt gctgcgcccc
    10501 ggcggcctcg actccacccg ccggttgctc aaggccctgg ccatcggccc cgccgaccgc
    10561 gtcgttgaat tcgcccccgg gctgggcctg accgcccggc gcatcctcgg ccttcgcccg
    10621 gccgcctata ccgccgtcga gcgcgacgag tccgccgccc gccggctccg cgactgcctg
    10681 accggtcccg cgatccagat cgtcaacgcc ggcgccgagg ccagcggcct gcccgaccag
    10741 tgcgccgagg tcgtcctggg cgaagccatg ctgacgatgc agccccccga gaagaaggcc
    10801 gccatcgtcc gcgaggcggc gcgcctgctg cgccccggcg ggcgctacgg ccttcacgag
    10861 atcgccctgc
//
LOCUS       DAOPCO010000132        10693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6014_length_10693_cov_6.343392, whole genome shotgun sequence.
ACCESSION   DAOPCO010000132 DAOPCO010000000
VERSION     DAOPCO010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6014_length_10693_cov_6.343392"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..538
                     /locus_tag="PLS90_10150"
     CDS             188..538
                     /locus_tag="PLS90_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HPK02803.1"
                     /translation="MNRTRTILTTTALALTLTLGTTGCETVRKHPGATTGAAVGAGTG
                     AVLGGMVGSQSDNTATGAIVGGVAGAGLGGVVGHQYDQIKEQEWRIDRLERRKDKWDD
                     RRHDRGRGRGHRRH"
     gene            761..1546
                     /locus_tag="PLS90_10155"
     CDS             761..1546
                     /locus_tag="PLS90_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02804.1"
                     /translation="MLANLTLPLSLGRLWITFVWALVLGAGVGVWVWMDDWFSTQAWT
                     MNLLLLPEREPDGPRREALRRTLLNEAVVREARWLSPDELLRSLERSQSELRPADLIP
                     SEPAWLPWVLELRPRDPLTHAAEIQALVARLGREADWELALWDAARLHELVALRGRLR
                     ALLGVFLGLVLVGGMAALLRTSWPRQAGWWLVGWSALLGAGGPAVLWMAAQLAGLPVE
                     PTCALAGAGAGFLLAAVFAPVLRVRGNRGVTLSITEADNERVR"
     gene            1530..1964
                     /locus_tag="PLS90_10160"
     CDS             1530..1964
                     /locus_tag="PLS90_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPK02805.1"
                     /translation="MREFGKVVVQNDRAPAPIGPYSQAIKCGEMVFVSGQLGIDPRTG
                     QLAGPDAASQAKVCLDHLQAILEMAGSSLGRVVKTTIFMVDLAEFEAVNKVYAEYFNF
                     EPPARSTIQVAALPKGARVEIEAIAMVTPSSPTEAQAGLGRF"
     gene            2005..2685
                     /locus_tag="PLS90_10165"
     CDS             2005..2685
                     /locus_tag="PLS90_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK02806.1"
                     /translation="MKLSVIIPVFNERRTIALILEKVRAVPIDKEIIVVDGASFDGTR
                     DILKQEETRPDTRVIYEPERRGRGRAIKEGLKAATGDIVIFQDADLELDPADYPALIA
                     PLQRGECDVVFGSRFLHGRPLMTFLQYWGNRVINAAVNLLYGTRLTDVETCYQVFRRE
                     VVQGMELENNDFAFTIELTVRLIRAGHPIREIPIRYIPRGRLEGKKIYWADGFAALWT
                     LVKYRFKS"
     gene            complement(2704..3513)
                     /locus_tag="PLS90_10170"
     CDS             complement(2704..3513)
                     /locus_tag="PLS90_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02807.1"
                     /translation="MSEIELKQRLAGIHIVPGQWRPIFPYEQIAWVTPPWLSSDYPWP
                     GKDYLWLDFPEAVFVDGKTHFLSHENPACAPPYTHLPKVPWKIIEGGLAFERRLPNGL
                     RFGGNVTRGDGALVRLEFFLRNESGAPLRRISVQTCCFLRGAAEFDAHTNENKLVRAG
                     GQGWLPIGKAARRPDGSGRWRLGFRGKGPAVADLPVVLARSAHARRFVAMSWGEQTAS
                     LMTNPRHPCIHADPAIPDLGPGETAAVRGVLAFIEGEPDEIDEDRLREWLG"
     gene            complement(3736..7659)
                     /locus_tag="PLS90_10175"
     CDS             complement(3736..7659)
                     /locus_tag="PLS90_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02808.1"
                     /translation="MTLGALPRRSAGDLSDPPSGADAPPPPRRRRRRLFRFTALLLLA
                     CLLLLLVVLLVPWSFDWVDRRVQSAWRKATGTGLTYSEATLRLLSGTFTVQSPRILDP
                     ATDRPLASFEQLTVNLPFAELVSLDSPIIIESISVSGPFAAAVALVDSRPRLLGDAAL
                     LLDRYQEHRRRLAAEEPEDQDARDRIGIRKIQFEPLSLRLLDVPTTAALADGTVTTAP
                     LTLDAITTHAVLLELDDARLELDFAGTLRPTGISLEAGFNGHPREEGLALTLNRVPGG
                     EDLDFDLAIARFLSRADLPWPAEPWFEVRGLAATGRIRRAADGPLHSRADLQLAELRV
                     QEAPASQIGDAATTSGRSLGPLSLALQAELSPDFKQLQFDSIELHNGSLALEAAGRLT
                     LARPFRYVVDVARLMLSDTALDLATVWLPGAGRMPQPPDARIRFAAHAAGDLLGLRPD
                     ALQGQLEISGFDWQQPSLPAPLQRLDLQASLTTESLRIVQASAQMGGIPLRIGGELRG
                     RPLLGEIDLLRLTWEASGALEDVTGLADRWMTEPARELTLTGHIEGRGEAVLESPFEG
                     AIRDALERARITGQIELDNVTARHPWLPWPVSDLNLRVNLAPERAEVTALTATAGETV
                     IEATGTLSGEPRFWSRPRIEGAGKVNGRLQDLAALAAKWVEPMPALAGGVRANFEGAT
                     TVGDWDDARVDGEVILSDVDFALVDPQFECTGLDARLNVTTDRVEIESLSAACLDRSV
                     QAEGRILGEPVFWRAPRLEFSAATAGPVDEIVAFVRNEAARFDAELPELPPAEGNAEI
                     RIAGEGSLARPATFELRAFLDLADYRTELNYPHIQGPLRLSGARVELARDRLTISDTT
                     GALGELPLSLAAEITPAGGEIQAGLSGALPSMKRHLPTLFEYMAVDGATSLSETIRWR
                     LREGAAPPADLEDVWRAIRPENPSADPDAWLASIRERYDLTADGSAQLQSAEITPSVF
                     PTRLTNISGTVRHDLERAWTPEPVAVDAGEGTTGARAVVEILFPLGGVTPELRFDIVS
                     AEHINVTAWTHEWNRLHPDEPHHAWPEDLQYDANLARRFIIRGQGRSRTAEYRQFQGE
                     DFRFRLGYDLHEGQPSMLTWSDVEAVLAGGVFRHHGSLFNHNLGLWLAIERGELQEIV
                     RMATQKDKQYGLASGIITGEVELGRRVGDVPTPWRGQGQLRIEESRFVSNKIFNSLGG
                     LLKLPFFEDISFSTIEGPFTVHGERFSSDGITFNGPIVNLKASGDVGPDEQLDLKVQI
                     EFLQIAGRIPLVAQALEIFNRLAGQVLMVRIRGTFDNPDIQPLGL"
     gene            complement(7770..10274)
                     /gene="gyrA"
                     /locus_tag="PLS90_10180"
     CDS             complement(7770..10274)
                     /gene="gyrA"
                     /locus_tag="PLS90_10180"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPK02809.1"
                     /translation="MNRQNITPVNIEDEMQASYIDYSMSVIVMRALPDVRDGLKPVHR
                     RIIYAMHDEGLQHNKQHSKCAGVVGEVLKKYHPHGDSAVYDALVRMAQDWNLRYPLID
                     PQGNFGSVDGDAPAAYRYTECRMTAIAELLLQDIEKNTVEFMPNFDGRVMEPTVLPAA
                     FPNLLVNGSAGIAVGMATNIPPHNLRETVTALKILIANPEATLPELMRVIPGPDFPTG
                     GYIYGRAGIREAYETGRGRIQLRARVMVEQQKSGKDSLIVTEIPYQVNKANLIAEIAT
                     LARDKKIQGISDIRDESDQNGMRIMIELRKGEVPQVIINQLYKMTQMQVTFGIILLAL
                     VDGRPRYLSLKRMLQCYLEHRREVVVRRTKFDLDKARRRIHIVEGLLIAVDNIDEVIK
                     IIRSSSSTDDARQSLKSRFGLTDEQTQAILDMPLRRLTGLEIEKLQQEHQELAALIEQ
                     LVAILADPNKVTTIIRDELDAVARKFGDERMTEIVDAEGELTVEDLIAEERMVITVTH
                     SGYIKRTPTSLYRRQRRGGKGASGVKPKEEDWVEHLFVGTTHNYIMFFTNTGKAYWLK
                     VWELPQAGRASRGRPIVNLLALEPGETIQAMIPIDKFSEDRYLVMCTRKGQVVRNSLD
                     LYSNPRKVGIKAINIADDDELIAVRMTNGHQQLLIATREGMAVRFDENDVRPMGRFVG
                     GVRGINLRGDDQVVGMIALRPESTVLTVCEKGYGKRSDAEDYRLTKRGGVGVINIRAT
                     ERNGKVIAVLDVIESDELILISEQGITIRSRVSDMRVIARATMGVRLMHLEEGDSLVG
                     VARIEEDKSVAGVADAADEGDEEADAEPEVDEGGEE"
BASE COUNT         1739 a         3637 c         3500 g         1817 t
ORIGIN      
        1 cactcttcga cccgggcgat cgttttatgt cccttatgtc cttttctgtc ccttgattcc
       61 caccggttcc tccaaaaaaa cgccccctca attcctcgga acaaaaccgc cccggccggc
      121 gtctgaatcg gcagacctcg ggaaccggat cgatcccccg gtttcgtttt tccaaggagg
      181 agtcatcatg aatcgcacca gaaccatcct gacgaccacc gcgctcgcgc tgacgttgac
      241 gttggggacg accggctgtg aaaccgttcg caaacacccg ggggcgacga ccggcgccgc
      301 cgtcggcgct ggaaccggcg ccgtcctggg ggggatggtc ggcagccaga gcgataatac
      361 cgccaccggc gcgattgtcg gcggcgtggc cggcgccggc ctgggcggcg tcgtcggcca
      421 ccagtacgac cagatcaagg agcaggaatg gcggattgac cggctcgagc gccggaagga
      481 caagtgggac gaccgccgcc atgatcgcgg ccgcggccgc ggccatcgcc ggcactgatt
      541 tccagacgac ccgccacgca gacacacccc gcattgaccc cgccaacttg agcccctcct
      601 cgaaatccgt cggatcggac ttaacagtcc ggtccgacgg attttcattt caacccggct
      661 cacatgcttc cccttttgtc ccttcccaat cgtcgaggcg atccttacca tgggcatgcg
      721 accgcggggg tcgccatccc tgggaaggat cgtccgtcgc gtgctggcca atctgaccct
      781 ccccctcagc ctgggccgtc tttggatcac gttcgtctgg gcgctggtgc tcggcgctgg
      841 tgtcggcgtc tgggtctgga tggacgattg gttttcaacc caggcgtgga cgatgaacct
      901 gctgctgctg cccgagcgcg aacccgacgg tccgcgccgc gaggcgctgc gccggacgct
      961 gctcaacgag gcggtcgtgc gcgaggcacg ttggctctcg cccgacgaac tgctgcgctc
     1021 gctcgaacgg tctcagtccg aactcaggcc ggccgacctg attccctccg agccggcctg
     1081 gctcccctgg gtgctcgagt tgcgcccccg ggatccgctg acgcacgccg cggaaatcca
     1141 ggcgctggtg gcccgactgg gccgcgaggc ggattgggaa ctggcgctgt gggatgcggc
     1201 gcgcctgcac gagctcgtgg cgctgcgcgg ccgcctgcgc gcgctgctcg gcgtcttcct
     1261 gggtctcgtc ctcgtcggcg ggatggccgc cctgttgcgt acatcgtggc ctcgtcaggc
     1321 ggggtggtgg ctggtcggct ggagcgcgct gctgggcgcg ggcgggccgg ctgtcctctg
     1381 gatggcggcg cagctcgccg gccttcccgt tgaaccgacc tgcgctctcg ccggcgccgg
     1441 cgccggtttc ctgcttgcgg ccgtgtttgc ccccgtgcta agagtgcggg gcaatcgcgg
     1501 cgtaaccctg agcatcaccg aggcggacaa tgagagagtt cggtaaagtc gtcgttcaga
     1561 acgatcgcgc gccggcgccg atcgggccct actcccaggc catcaagtgc ggcgagatgg
     1621 tcttcgtctc cggccagttg gggatcgatc cgcgcaccgg tcagctcgcc ggccccgacg
     1681 cggcctcgca ggccaaggtc tgcctcgatc acctgcaagc gattctggag atggcgggct
     1741 ccagcctggg ccgggtcgtc aagaccacga tctttatggt ggacctggcc gagttcgagg
     1801 cggtcaacaa ggtttacgcc gaatatttca atttcgagcc gccggcgcgc tcgacgattc
     1861 aggtcgccgc tctgcccaag ggcgcgcgcg tcgagatcga ggcgatcgcg atggtgaccc
     1921 cctcctcgcc gaccgaagcc caggccggcc tcggccggtt ctgatggccc ggcgcgcggg
     1981 cccatcccct ttccgccgcg cggcatgaag ctctcggtca tcatccccgt tttcaacgag
     2041 cggcggacga tcgcgctcat cctggagaag gtgcgcgccg tcccgatcga caaggagatt
     2101 atcgtcgtcg acggcgccag cttcgacggc acgcgggata ttctcaagca ggaggaaacg
     2161 cgccccgaca cccgcgtgat ctacgagccg gagcggcgcg ggcgcggccg cgcgatcaag
     2221 gagggcctca aggccgcgac cggcgacatt gtcatcttcc aggacgccga tctcgaactc
     2281 gacccggccg actaccccgc gctgatcgcc cccctccaac gcggcgagtg cgacgtcgtc
     2341 ttcggctcgc gtttcctgca cgggcgaccg ctgatgacct tcctgcaata ctggggcaac
     2401 cgcgtgatca acgcggcggt caatctgctc tatggcacgc ggctgaccga tgtcgaaacg
     2461 tgttatcagg tctttcgccg cgaagtcgtc cagggcatgg agctggagaa caacgatttc
     2521 gccttcacga tcgaactgac cgtgcggctg atccgcgccg gccatccgat ccgtgagatt
     2581 ccgatccgtt acatcccgcg cggccggctt gagggcaaga aaatctactg ggccgacggc
     2641 ttcgccgccc tgtggacgct ggtcaaatat cgcttcaaat cctgaaggat caccgggccc
     2701 ttctcagccc agccactcgc gcagacggtc ctcgtcgatc tcgtccggct cgccttcaat
     2761 aaacgccagc acgccgcgga cggcagcggt ctcgcccggc ccaagatccg gaatcgccgg
     2821 atcggcgtgg atgcagggat ggcgcgggtt ggtcatcagc gaggcggtct gctcgcccca
     2881 gctcatcgca acgaaccgcc gggcgtgcgc cgagcgagcc agcaccacgg gcaggtcggc
     2941 cacggccggt cccttgccgc ggaagcccag ccgccagcgg cccgagccgt cgggccggcg
     3001 cgccgccttg ccgattggca gccagccctg cccgcccgcg cggacgagct tgttctcatt
     3061 ggtgtgggcg tcgaattcgg cggccccgcg caggaagcag cacgtctgga cgctgatccg
     3121 gcgcagcggg gcgccgcttt cgttgcgcag gaaaaactcc agccggacga gcgcaccatc
     3181 gccgcgggtg acgttgccgc cgaaccgcag accgttgggg agccggcgct cgaaggccag
     3241 acccccttcg ataattttcc atgggacttt gggcaggtgc gtgtagggag gcgcgcaggc
     3301 cggattttcg tggctgagga agtgcgtctt gccgtcgacg aacacggctt cgggaaagtc
     3361 gagccagaga taatccttgc ccggccaggg atagtcgctg ctgagccagg gtggcgtgac
     3421 ccaggcaatt tgctcgtagg ggaagatcgg ccgccattgc ccggggacga tgtggatgcc
     3481 cgccaggcgt tgcttcagtt caatttcgct catcgccatt ccatcctgga attgcccggc
     3541 ctcggccgat tcgtccctga cccggcgcca ttgcgcaaat tctaccccga tatcccaaca
     3601 aaatggatca gaaacggcgc ggaggggtcc ggaacgctaa cgtaatcgac cgcttcgaac
     3661 gaccgaggcc ctgccggact cagtccactc ccagtcccat ccgggacgca gagccgttca
     3721 ttttcttcgg aaaccttaca atcccaacgg ttgaatatcc ggattgtcga acgtgccccg
     3781 gatccggacc atcagaacct gcccggcgag ccggttgaaa atctcgagcg cctgcgccac
     3841 cagcgggatc cggccggcga tctgcagaaa ctcgatctgg actttcaggt cgagctgctc
     3901 gtccgggccg acatcccccg atgccttgag attcacgatc gggccgttga acgtgatccc
     3961 gtcgctggag aaccgctcgc cgtggaccgt gaacggtcct tcgatcgtcg agaacgaaat
     4021 atcctcgaaa aacgggagct tcagcaggcc gcccagactg ttgaaaatct tgttggagac
     4081 aaaccggctc tcctcgatcc gcagttgccc ctggccccgc cacggcgtcg gcacgtcgcc
     4141 gacgcggcgg cccagctcca cttcccccgt gatgatgccc gaggccaggc cgtattgctt
     4201 gtccttctgc gtcgccatcc ggacgatttc ctggagctct ccacgctcga tcgccagcca
     4261 caggccgagg ttgtgattga acagcgagcc atgatgccgg aagacgcccc ccgccaggac
     4321 cgcctcgaca tcggaccacg tcaacatcga gggctggcct tcgtgaaggt cgtagccgag
     4381 gcggaaccgg aagtcctccc cctggaactg gcgatactcc gcggtgcgcg atcggccctg
     4441 gccgcgaatg atgaaccggc gcgcaaggtt ggcgtcgtat tgcagatcct cgggccatgc
     4501 atgatgcggt tcgtccggat gcaaccggtt ccattcatgg gtccaggcgg tcacgttgat
     4561 gtgctcggcc gagacgatgt cgaaccgcag ctcgggcgtt acgccgccca gcggaaaaag
     4621 aatctcgacg acggcgcgcg ccccggtcgt cccttccccc gcatccaccg ccaccggttc
     4681 cggcgtccag gcgcgttcca gatcatgccg gaccgtgcca ctgatgttgg tcagccgggt
     4741 cgggaagacg ctgggcgtga tctcggcgga ttgcagctgc gcgcttccgt cggcggtcag
     4801 gtcgtaacgt tcgcggatgg acgcgagcca ggcgtcggga tcggcggagg gattctctgg
     4861 acggatggcc cgccagacgt cctccagatc ggcggggggc gcggcccctt cgcgcagtcg
     4921 ccagcggatc gtctcgctca gactcgtcgc gccgtcgacg gccatgtact cgaacaacgt
     4981 cggcagatgc cgcttcatcg acggcagcgc gccgctcaac ccggcctgaa tctcgccccc
     5041 cgcgggcgtg atctcggcgg cgagcgacag cggcagttct ccgagcgccc cggtcgtgtc
     5101 ggagatcgtc agccggtcgc gggcgagctc cacccgcgcg ccgctcagcc gcagcggccc
     5161 ctggatgtgc ggataattca gctcggtccg gtaatcggcg agatcgagga acgcccgcag
     5221 ttcgaacgtc gcgggccgcg ccagggatcc ctcgccggcg atccggatct cggcgttccc
     5281 ctcggccgga ggcagctccg gcagctcggc atcgaaccgg gcggcctcgt tgcggacgaa
     5341 ggcgacgatc tcatcgaccg gtccggcggt ggcggcgctg aattccaggc gcggcgcccg
     5401 ccagaagacc ggctcgccga ggatccgccc ctcggcctga accgaccggt cgaggcaggc
     5461 cgcgctgagc gattcaatct caacgcggtc ggtcgtcaca ttcagccggg cgtcgagccc
     5521 ggtgcattca aactgagggt caaccaacgc gaagtcgacg tcggacaaaa tgacctcgcc
     5581 gtcgacgcgc gcgtcgtccc aatcgccgac cgtggtcgcg ccctcaaagt tcgcccgcac
     5641 gccgcccgcc agggcgggca tcggctccac ccatttcgcc gccagcgcgg ccagatcttg
     5701 gagccggccg ttgaccttgc cggccccttc gatccgcggc cgactccaga agcgcggttc
     5761 gcccgagagg gttccggtcg cctcgatcac cgtttcgccg gcggtggccg tcagcgccgt
     5821 cacctccgcg cgctcgggag cgaggttgac gcgcaggttc aggtccgaaa ccggccacgg
     5881 cagccatgga tggcgcgcgg tgacgttgtc cagctcgatc tgcccggtga tccgagcgcg
     5941 ctccagcgca tcgcggatcg ccccctcgaa cggcgattcg agcaccgctt cgccccgccc
     6001 ttcgatgtgg cccgtcaacg tcagttcgcg cgccggttcc gtcatccagc ggtcggcaag
     6061 gcccgtcacg tcctccagcg cgccgctggc ttcccacgtc agccggagca gatcgatctc
     6121 gcccagcagc ggccggccgc gcaactcccc cccgatccgg agcgggatgc cgcccatctg
     6181 ggccgaggcc tgcacgatcc gcagcgattc ggtcgtcagc gatgcctgca ggtcgagccg
     6241 ctgcaacggc gccggcagcg aaggctgttg ccaatcgaaa ccgctgattt caagctggcc
     6301 ctgcagggca tcgggccgca gcccgagcaa gtcgccggcg gcatgcgccg cgaagcggat
     6361 gcgcgcgtcg ggcggctggg gcatgcgccc ggcgccgggc agccagaccg tcgccagatc
     6421 cagggccgtg tcgctcaaca tcagccgggc gacgtcgacg acgtatcgga acggccgggc
     6481 gagcgtcagc cgcccggcgg cttccagcgc cagcgagccg ttgtgcagct cgatcgaatc
     6541 gaactgaagc tgcttgaaat cgggcgaaag ctccgcctgc aacgccaggc tgagcgggcc
     6601 gaggctgcgc cccgacgtcg tggcggcgtc gccgatctgg ctcgccgggg cttcctgaac
     6661 gcgcagttcg gccagctgca ggtcggcccg cgaatgcagg gggccgtcgg ccgcgcggcg
     6721 gatgcgtccc gtcgcggcca ggccgcgcac ctcgaaccac ggctcggccg gccaggggag
     6781 atcggcgcgg ctcaggaacc gcgcgatcgc cagatcaaaa tcgagatcct cgccgccggg
     6841 cacgcggttc agcgtcagcg cgagaccctc ctcgcgcgga tgaccgttga atcccgcctc
     6901 gagcgagatc cccgtcggcc tgagcgtgcc ggcgaaatcg agctccagcc gggcgtcgtc
     6961 cagctccagc agcaccgcgt gcgtcgtgat ggcgtccagt gtcagcggcg ccgtcgtgac
     7021 ggtcccgtcg gccagcgcgg cggtcgtcgg cacgtcgagc aggcgcaggc tgagcggctc
     7081 gaattgaatc ttccggatgc cgatgcggtc gcgcgcgtcc tgatcctcgg gctcctccgc
     7141 cgccaaacgc cggcggtgct cctggtaacg gtccagcagc agggcggcgt cgccgagcag
     7201 ccgcggccgg gaatcgacga gcgcgacggc ggccgcgaac gggcccgaaa ccgagatgct
     7261 ctcgatgatg atcggcgaat cgagcgagac gagctcggcg aaggggaggt tgacggtcag
     7321 ttgctcaaac gacgccagcg gccggtcggt cgccggatcg aggatgcgcg gcgactgaac
     7381 agtgaacgtc cccgacagca gccgcagcgt ggcctcggaa taggtcaggc cggtgccggt
     7441 cgccttgcgc caggcgctct ggacgcggcg atcgacccag tcgaacgacc acggcacgag
     7501 cagcacgacg agaagcagga gaaggcaggc gaggagcagg agcgcggtga aacgaaagag
     7561 ccgtcgtcgg cggcgcctcg gtggaggcgg cgcgtcggcg ccggacgggg gatcggacag
     7621 gtcgcccgcc gagcggcggg gcagcgcacc gagcgtcata acacgggctg tttcaaggga
     7681 ctgagtgaac cggcgatccg gcgcaccgga accggctcgg cgtgttcgcc gcaaagctca
     7741 gccagggaag caacaagagt gcccggccgc tattcctcgc cgccctcgtc gacctccggc
     7801 tccgcgtccg cttcctcgtc gccctcatcc gccgcgtcgg cgacgcccgc caccgacttg
     7861 tcctcctcga tgcgcgccac gccgaccagg ctgtcgcctt cctccagatg catcaggcgg
     7921 acgcccatcg tcgcgcgggc gatgacgcgc atgtcggaga cccgcgagcg gatcgtgatg
     7981 ccctgctccg agatgaggat cagctcgtcg ctctcgatga cgtccaggac ggcgatcacc
     8041 ttgccgttgc gctccgtggc gcggatgttg atcacgccga cgccgccgcg cttggtcagc
     8101 cggtagtcct cggcgtcgct ccgcttgccg tagccctttt cgcacacggt cagcaccgtc
     8161 gattcggggc gcagggcgat catgccgacc acttggtcgt cgccgcgcag gttgatgccg
     8221 cgcacgccgc cgacgaagcg gcccatcggc cgcacgtcgt tttcatcgaa tcgcacggcc
     8281 atcccctcgc gcgtggcgat cagcaattgc tgatgcccat tggtcatccg caccgcgatc
     8341 agctcgtcgt cgtcggcgat attgatcgcc ttgatgccga ccttgcgcgg gttggagtag
     8401 aggtccagcg aattgcgcac cacctgcccc ttgcgcgtgc acatgacgag ataacggtcc
     8461 tcgctgaact tgtcgatcgg gatcatcgcc tggatcgttt cgcccggctc cagcgcgagc
     8521 aggttgacga tcggccgccc gcgcgaggcg cgccccgcct gcggcagctc ccacactttc
     8581 agccagtacg ccttgcccgt gttcgtgaag aacatgatgt agttgtgggt cgtccccacg
     8641 aacaggtgtt caacccagtc ttcctctttg ggtttgaccc cgctggcccc cttgcccccg
     8701 cggcgctggc ggcggtagag gcttgtcggc gtgcgcttga tgtagccgct gtgcgtgacg
     8761 gtgatcacca tccgctcctc ggcgatcagg tcctcgaccg tcagttcgcc ttcggcgtcg
     8821 acgatctcgg tcatccgctc atcgccgaac ttccgggcca ccgcgtccag ctcgtcgcgg
     8881 atgatcgtgg tcaccttgtt ggggtcggcc aggatcgcga caagctgctc gatgagggcg
     8941 gccagttcct ggtgttcctg ctggagcttt tcgatttcca gtccggtcag ccggcgcagc
     9001 ggcatgtcca ggatcgcctg cgtctgctcg tcggtgagcc cgaagcgtga tttgaggctc
     9061 tggcgcgcat cgtcggtgga ggacgacgac cggatgatct tgatcacctc gtcgatgttg
     9121 tcgacggcga tcagcagacc ctcgacgatg tggatccgcc ggcgcgcctt gtccagatcg
     9181 aacttcgtcc ggcgcacgac cacctcgcgg cgatgctcca gataacactg cagcatgcgc
     9241 ttgagcgaaa ggtagcgcgg acggccgtcg acgagcgcga gcaggatgat gccgaacgtg
     9301 acctgcatct gcgtcatctt gtaaagctgg ttgatgatga cctgcgggac ctcccccttg
     9361 cgcagctcga tcatgatccg catgccgttc tggtccgact cgtcgcggat atccgagatg
     9421 ccctggatct tcttgtcgcg ggccagcgtc gcgatctcgg cgatcaggtt ggccttgttg
     9481 acctgatatg gaatctcagt aacgatcagc gaatccttgc ccgatttctg ctgttcgacc
     9541 atcacgcgcg cgcgcagttg aatccggccg cggccggtct cgtaggcctc gcgaatgccc
     9601 gcgcggccgt agatgtagcc gccggtgggg aaatcgggcc ccggaatgac ccgcatcaat
     9661 tcgggcagcg tcgcctcggg attggcgatc aggatcttga gcgccgtgac cgtctcgcgc
     9721 aggttgtgcg gggggatgtt ggtcgccatc ccgacggcga tccccgccga gccgttgaca
     9781 agcagattgg ggaacgcggc cggcaggacg gtcggttcca tcacgcgccc gtcgaaattc
     9841 ggcatgaact cgacggtgtt cttctcgatg tcctgcagca gcagctcggc gatcgccgtc
     9901 atccggcact ccgtgtaacg gtaggctgcc ggcgcgtcgc catccaccga cccgaagttg
     9961 ccctgcggat cgatcagcgg gtagcgcagg ttccagtcct gcgccatgcg gaccagcgcg
    10021 tcgtagaccg cgctgtcgcc gtgcggatgg tatttcttga gcacctcgcc gacgacgccc
    10081 gcgcacttgc tgtgctgctt gttgtgctgc aaaccctcgt cgtgcatggc ataaatgatc
    10141 cgccgatgga ccggcttgag gccgtcgcgc acatcgggca gcgcgcgcat cacgatcacc
    10201 gacatcgagt agtcgatgta ggaggcctgc atttcgtctt cgatgttgac cggggtaatg
    10261 ttctggcggt tcatttcctg catggatcgg gcctcgggac aagcgagaat cggtcgggga
    10321 gggcgcggca gcgttcccgg cgggtcaatt tgaccggatg cgcggggctg gaacgacccc
    10381 gcgaaccagc ccctccacca atttagggga tcgggaagga agaagcaagg acagggaacc
    10441 gcccgaaacc ccgcgaaaac ccgcaaagcg gcaaggaata aaggccccca ggatgaaatt
    10501 tcgggatcgg tatcggaatc gggaacattc tcggaatcga aaaaccccgc aaccgcacgg
    10561 aactccgcgg cgactccagt ttgccaaccg catcagcggc tggttttctg atccacggcg
    10621 aatgtcggaa tggttcaaat ggatcaccag aacgagcgaa gccggacgag ttttcgtgga
    10681 agggccaaat gac
//
LOCUS       DAOPCO010000133        10570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6112_length_10570_cov_4.457632, whole genome shotgun sequence.
ACCESSION   DAOPCO010000133 DAOPCO010000000
VERSION     DAOPCO010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6112_length_10570_cov_4.457632"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..748)
                     /locus_tag="PLS90_10185"
     CDS             complement(125..748)
                     /locus_tag="PLS90_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02810.1"
                     /translation="MFSRRAFTLIELLIVVAIIAILAAIAVPNFLEAQTRAKVARVRT
                     DFRIITVANESYYVDNNKYAQTAIFQGETAAKSGNSIQNSLRACTNAGPTELTTPIAY
                     LTKYPWDIFTIISPWSSSQVVNRETMGRHDYPYYYLNVESYVAQYGASDPGQYWVIEH
                     RKGAKYYLQSWGPLGQGWTFPYDPTNGTISPGTIGRWGPQRPEGFGN"
     gene            complement(904..1596)
                     /locus_tag="PLS90_10190"
     CDS             complement(904..1596)
                     /locus_tag="PLS90_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02811.1"
                     /translation="MSKPNHLLHGHLIFGFCLALALIGAPAQAFDELVGDFYAYEGDI
                     DPADHEPPIVYNTDGTIAKTSTDGNLLTFTDLPQYDGLRFTTAFNPPNFTTTGVMTME
                     MQIRVTTGVIFFIAGDGDSRISFGINPNGVSFYGLEDDAAIVTDFTQLRRLRVVVEGG
                     QLSAYVDGEAENFVDHLNLTGGSFPAADLYANHAGQVTNAQLDYIRSSEQALHPELGE
                     VVNAATTWPAYQ"
     gene            complement(1727..2407)
                     /locus_tag="PLS90_10195"
     CDS             complement(1727..2407)
                     /locus_tag="PLS90_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02812.1"
                     /translation="MTHNLTIKGCLLALALAWIGFVTPGFCQTETPGDHYAYEGDVLP
                     PDHVPAINYNTAGTADKTSTDGSLLTLTDLPQYDGIRFTAAFTPTGFSAATMTMELRV
                     RVTSGAMTFVVGDSSKSFQFGITPTGVTFPSRIDPTGAIVADFTQFRRLRIVIENGQL
                     SAYVQDESNYIQGADITGGAFPAVDFYADHAGQATNCQLDYIRSTTQVLHPEQWWLPV
                     ELNSFVVE"
     gene            3066..3635
                     /locus_tag="PLS90_10200"
     CDS             3066..3635
                     /locus_tag="PLS90_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HPK02813.1"
                     /translation="MNDKPRATNRTTEKSARDELGIIYEEGADTPAGRGELGPLALFL
                     VLMGAAGLVGYLLGGGMRGDWYGGLAKPAITPPAAIFGPVWTILYILIAIAGWLVWRR
                     RREIIIRPAMTAWGVQLGLNALWPGLFFGLQRTGWAFLEILALWIAIALTVRQFERIR
                     RAAGWLLAPYLLWVTYAAVLNFAIWRMNP"
     gene            complement(3654..3977)
                     /locus_tag="PLS90_10205"
     CDS             complement(3654..3977)
                     /locus_tag="PLS90_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02814.1"
                     /translation="MRVRLTATYAAAGMLALALLPASGCALYRDDKCWVPPEQYAEAR
                     EYYIQTGSLELVGEYMKDNQWRRCRRNEVLYRLRKEFEALPEELPEVIPPEAGSAEET
                     PAPTS"
     gene            complement(4009..5007)
                     /locus_tag="PLS90_10210"
     CDS             complement(4009..5007)
                     /locus_tag="PLS90_10210"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018757767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPK02815.1"
                     /translation="MPDLIPVGFLGLGTFVPQRVLTNHDLEQMVETSDEWIVTRTGIR
                     ERHICADDECTSDMATAAARAAIADAGLTPDQIDLIICGTYTPDHLTPPTACIVQANL
                     GVTRPIPAFDLSAACSGFVYALSVGVNFVQTGMYRHVLVVGADAMSKVLDYQDRNTCV
                     LFGDGAGAVVLGRVEPGRGLIGHTIGADGRGAEQIIMHAGGSREPFSLDALNNRRQYM
                     RMAGREVYKFATSIVAPAIEDALRNGGNGLCVQDLELIIPHQANMRIIEAAAKKLGLP
                     IDRFVVNIDRYGNTSGATVPLAMGDARAAGRLQPGTLFALVAFGGGLTYGASIWRW"
     gene            5427..6359
                     /gene="accD"
                     /locus_tag="PLS90_10215"
     CDS             5427..6359
                     /gene="accD"
                     /locus_tag="PLS90_10215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPK02816.1"
                     /translation="MTWFRKRKFTTLRPPSQRSRIPEGMWLKCSNCLEIITNKDWEEN
                     LKVCPRCGHHERIGARERIRQLVDPGSFEEYDAGLVSTDPLQFVDLKPYREHIEESRR
                     KSGENDAIVCGMGRIETMPLSLGVMEFAFRGGSMGSVVGEKVARTLERGLEHRVPVLI
                     VCASGGARMQEGILSLMQLAKTSALIARLGQAGLPYFVLLTHPSYAGIMASFGSLGDV
                     ILAEPEALIGFAGPRVIEQTIKQILPPGFQKSEFVQEHGFVDLVLPRKELRPTLARLM
                     RFFGSEPATASETRPAEAERASENSARIIESFGP"
     gene            6547..6846
                     /locus_tag="PLS90_10220"
     CDS             6547..6846
                     /locus_tag="PLS90_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPK02817.1"
                     /translation="MRASQSIPLNVAEENGKFAEADRRRSFEIARGSALECAAIRDVL
                     MVGNAMDEMAHRKGKDQLDRIVAMLTRMGGRSCSVREEQASYLDSGSDSDTDTDA"
     gene            6901..8046
                     /locus_tag="PLS90_10225"
     CDS             6901..8046
                     /locus_tag="PLS90_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009629470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK02818.1"
                     /translation="MEYRRFGKTELKLSVITLGGMRYPSSGADPQTTETLPDEALEAC
                     YQCTQRALDLGINHIETAHGYGLSERLYGATWRRLRQRRDEFYLMTKGAPATWDDAMR
                     QVEVQLGRLQTDRIDLYGWHGINTPEINQSATAKGGPVEALHRLREEGVIGHVGFSTH
                     GPVGVILDAINTGLFEFVNLHCYYFFQRNLPAVIQAGLRDMGVFIISPNDKGGQLFNA
                     PDRLRGICAPLTPIQFNARFCLSHPQIHTLSFGIDKPEFFDEAMGVLNGGYYLSPADR
                     RTKLELDRQIERLGDTYCTGCYACLPCPEEINIPEVLRFRNMWKGYDMEAFGRYRYNM
                     LQQKGHWFPGSFASACNDCGDCLPRCPVRLNIPELLRETHAGLATTN"
     gene            8267..8839
                     /locus_tag="PLS90_10230"
     CDS             8267..8839
                     /locus_tag="PLS90_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK02819.1"
                     /translation="MTHGLKKTAATAAWALLAIAVAPSAQALTLQGGSLFATADEEIT
                     ITLSGISTAYRDRLTLADSDDVLFDSQQSALGANRSLGMMPSGGELVFRLNVREPDGD
                     SYAWFSGPASRNADNTQHARILDLGGNRWRLSWEDLSLFDPLFDGDFNDLMFIVESRP
                     IPVIPEPATLSLVGLGLGATGWVRRRRKRR"
     gene            9277..9843
                     /locus_tag="PLS90_10235"
     CDS             9277..9843
                     /locus_tag="PLS90_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4114 domain-containing protein"
                     /protein_id="HPK02820.1"
                     /translation="MRSLPKVLLALALMSGLVLPAHALTLSGNSLFTSGLPEVVTVTY
                     LSSEAAYTNLMFETQPNTQLLFATNTATSGQTVSLGEIAANTELVFRLDSFFNGNQVE
                     QWYSGPGSRNIDGTVHARITFIGNDTWQLAWEDLNFNESGYDGDFNDMIVNIKSAVVP
                     EPATLALVGLGVGSLGLLRRRSRKPAKA"
BASE COUNT         1856 a         3081 c         3704 g         1929 t
ORIGIN      
        1 cgagcagtcc gctcaattgg gttggcctat tggacacggt gaacaccttt ccagagaatg
       61 attgggttga tatgcttgct gccgcgcccg ctccccgacc cgggccgggg agcgggtcgc
      121 gccgtcagtt gccgaagccc tcgggccgct gcggccccca tcgtccgatc gtgcccgggc
      181 tgatggtgcc gttggtcggg tcgtagggga aagtccagcc ctggccaagc ggaccccagg
      241 actgcaggta gtatttggcg cccttgcgat gttcgatgac ccagtactgg ccggggtccg
      301 acgcgccata ttgcgccacg tagctttcga cattgaggta atagtagggg tagtcatgcc
      361 ggcccatggt ttcgcggttg acgacctggc tcgaggacca ggggctgatg atggtgaaga
      421 tgtcccaggg gtacttggtc aggtaggcaa tcggcgtggt cagctcggtc gggccggcgt
      481 tggtgcaggc gcgcaggctg ttctgaatgc tgttgccgga tttggcggcg gtttcgccct
      541 ggaagatggc ggtctgggcg tacttgttgt tgtcgacgta atagctttcg ttggcgacgg
      601 tgatgatgcg gaaatcggtg cggacgcggg cgaccttggc ccgggtctgg gcttcgagga
      661 agttgggcac cgcgatcgcc gccaggatgg cgatgatcgc tacgacgatc agcaattcga
      721 tcagtgtgaa ggcgcggcgc gagaacatgg gattgtcctt tctccgggag cggccggata
      781 agaatggggg accgggccaa gccgccggcc gcacccgggg gattttcaat cagggatact
      841 cgagtgtcca ttcctcagga tgcatgtctt ttcggcagcc ggcccgatcg gccgggtcgg
      901 ggattattga tacgccggcc aggtcgtggc ggcgttgact acctcaccga gctctggatg
      961 gagcgcctgc tcggaggagc ggatgtagtc cagttgcgcg ttggtcacct ggccggcatg
     1021 gttggcatag aggtccgccg ccgggaacga gccgccggtg agattgagat ggtcgacgaa
     1081 gttttcggct tcgccgtcga cgtaggccga aagctgaccc ccctcgacga ccacgcgcag
     1141 gcggcgcaac tgggtgaagt cggtgacgat cgcggcatca tcctcgaggc cgtaaaacga
     1201 gacgccgttg ggattgatgc cgaaactgat ccggctgtcg ccgtcgcccg cgatgaagaa
     1261 gatcacgccg gtggtcacgc ggatctgcat ctccatggtc atgacgccgg tggtggtgaa
     1321 gttgggcgga ttgaaggccg tggtgaagcg caggccgtcg tactgcggca ggtcggtgaa
     1381 cgtcagcaga ttgccgtcgg tcgaggtctt ggcaatggtt ccgtcggtat tgtagacaat
     1441 cgggggttcg tggtcggccg gatcgatatc cccctcgtag gcgtaaaagt cgcccaccag
     1501 ttcatcgaag gcctgagccg gcgcgccgat gagggccagc gccaggcaga agccgaaaat
     1561 cagatggccg tggagcagat gattgggctt ggacatcgcg agtccctcct tcaggtcaat
     1621 tgggggtttg caaggcgtga ttcgggcatg ggttgccggt cagccgccgc gccaggcgac
     1681 tggacgttct ctggcgcgac ggcgaacggc gcgagcaggg gggattttac tccacaacaa
     1741 acgagttcag ctcgacgggc agccaccact gctcgggatg caggacttgc gtggtggaac
     1801 ggatgtagtc gagctggcag ttggtcgcct ggccggcgtg atcggcatag aaatcgacgg
     1861 cggggaacgc gccgccggtg atgtcggcgc cctggatgta gttggactca tcctggacgt
     1921 aggcggaaag ctggccgttc tcgatgacga tgcgcaggcg gcggaactgg gtgaagtcgg
     1981 cgacgatcgc gccggtggga tcaatccgcg aagggaaggt caccccagtg ggcgtgatcc
     2041 cgaactggaa gctcttggag gaatcgccga cgacgaaggt catggcgccg ctggtcacgc
     2101 gcacgcgcag ttccatcgtc atcgtcgccg cggagaaacc ggtcggggtg aacgccgccg
     2161 tgaaccggat gccgtcatat tgcggcaggt cggtgagcgt cagcaggctg ccgtcggtgg
     2221 aagtcttgtc ggcggtgccc gccgtattgt agttgatcgc gggaacgtga tccgggggca
     2281 gaacgtcgcc ttcataggca taatgatcgc ccggagtctc ggtctggcag aagcctggcg
     2341 tgacgaagcc gatccaggcc agcgccagcg ccagcaaaca gcctttgatc gtaaggttgt
     2401 gggtcatcgt tgctctctcc caattcgatt caaacagtgg tccagtggtg cacgcccctc
     2461 tcgcatacaa caccttcgga agtggcgccc gagcattgat tatccgtgtg tgcgtggagc
     2521 cctcctttgc ggatggaatg ccgggtggac agtcggccgc ggggccgggc ggtcggtggc
     2581 cgcaccctgc cccgccatct ggctgccgaa gtgacagggc gccggcgctg cgtgtgctgt
     2641 cggtcggatc ggatgggaag agccgcgcgc ttgcatggcg gtggttcctg cccggagaat
     2701 gaggttctgc ccggtcggcg aggcagccga tagacaggca tgtcatttcg cttaaaatta
     2761 aagatacatg tcatgtttct agtcgtcaac catgaatcca cgcggcgcgc ggggaaatct
     2821 tgcggccgcg ggcagatttc agacgtgttg gtcgtatcct gaaacggcgg tcggtcattg
     2881 gcgaaagtcg agggcacggc ttgatttcgc gagccgagga cgagggacaa gagccgggag
     2941 ccggattgtg ggggagccgt tgggggcgaa tacgaggacg agaacgaagg acggattctg
     3001 agagagggat gagttggaag aatcgggggc ggggtgacat aatggcggcc aacgaaaggc
     3061 aactcgtgaa cgacaaaccc cgcgcgacga acaggacgac cgaaaaatcg gcccgggacg
     3121 aactgggaat catctacgag gaaggggcgg atacgcccgc cgggcggggc gagttgggcc
     3181 cgctggcgct gtttctcgtg ctgatgggag ccgccggact cgtcggctat ctgctggggg
     3241 gcgggatgcg gggcgactgg tacggcgggc tggccaagcc ggccatcacg ccccccgcgg
     3301 cgatcttcgg accggtctgg acgatcctgt acattctgat cgcgatcgcg ggctggctgg
     3361 tctggcggcg ccgacgtgag attatcatcc gcccggccat gaccgcctgg ggggttcagc
     3421 ttgggttgaa cgcgctctgg cccggcctgt tcttcggcct gcagcggacc gggtgggcgt
     3481 tcctggagat cctggcgctg tggatcgcga tcgcgctgac ggtgcggcag ttcgagcgta
     3541 tccgccgcgc ggcgggttgg ctgctggcgc cctatctgct gtgggtgacc tatgcggccg
     3601 tgttgaattt cgcgatctgg cgcatgaatc cgtagcgggg cgaccgaacg gcgtcaggaa
     3661 gtcggcgccg gcgtttcctc ggcgctcccg gcctccggcg ggataacctc gggcaattcc
     3721 tcgggcagcg cctcgaattc cttgcgcagg cggtagagga cctcgttgcg gcggcagcgg
     3781 cgccactggt tgtccttcat atattcgccc accagttcga gcgagccggt ctggatgtag
     3841 tattcacggg cttcggcgta ctgttcgggg gggacccagc atttatcgtc gcgatagagg
     3901 gcgcatcccg acgccggcag gagcgccagc gccagcatcc cggcggccgc gtaggttgcc
     3961 gtcaatcgaa cccgcatggt ggacctttcc gtgtggtgcc gccgcccgtc accagcgcca
     4021 gatgctggcg ccgtaggtga gaccgccgcc gaaggcgacg agggcgaaga gcgtgcccgg
     4081 ctggagcctg ccggcggcgc gggcgtcgcc catggccagg gggacggtgg cgccggaggt
     4141 attgccgtag cggtcaatgt tgaccacaaa gcggtcaatg ggcaagccga gttttttggc
     4201 ggcggcctcg atgatgcgca tgttggcctg atgcgggatg atcagttcga ggtcctggac
     4261 gcacaggccg ttgccgccgt tgcgcagggc gtcttcgatg gcgggggcga cgatgctggt
     4321 ggcgaacttg tagacttcgc ggccggccat gcgcatgtac tggcggcgat tgttcagcgc
     4381 gtccaggctg aacggctcgc gcgagccgcc ggcatgcatg atgatctgtt cggcgccgcg
     4441 gccatcggcg ccgatggtgt ggccgatcag gccgcggccg ggctcaacgc ggccgaggac
     4501 gacggcgccg gcgccgtcgc cgaacaggac gcaggtgttg cgatcctggt agtccagcac
     4561 cttgctcatc gcgtcggcgc cgacgaccag gacgtggcga tacatgccgg tctggacgaa
     4621 attgacgccg acggacaagg catagacgaa gccgctgcag gcggccgaca gatcgaacgc
     4681 cggaatcgga cgcgtcacgc cgagattggc ctgcacgatg caggcggtgg ggggggtcag
     4741 gtggtcgggg gtataggtgc cgcagatgat caggtcgatc tgatcggggg tcaggccggc
     4801 gtcggcaatg gcggcgcggg cggccgccgt ggccatgtcg gaggtgcact catcgtcggc
     4861 gcagatatgg cgctcgcgga tgccggtgcg cgtgacgatc cattcgtcgc tggtctccac
     4921 catctgttcc aggtcgtggt tggtcaggac gcgttggggg acgaaggtgc ccaggcccag
     4981 aaagcccaca ggaatcaagt caggcattcc gctacagctc gctttgggga aatcgacatg
     5041 actggagaga acccggcagt gagggacagg gcgcgcgcgg ccgcccgatc aatcatgccc
     5101 gcaggcatgc caggacagca gattgccaag tcctgaatgc aaaaacaagt caatctttcc
     5161 atgaagaatc ggcctgggtt cgggcggttg gcgacgcggg aaatgcgctc tcgtctgtcg
     5221 ttctcacgct gaatccgtcc gatccgtcgg atccgacgga tttcgcggcg caggacggcc
     5281 gtgcgggttg tttgacttcg cgcgcgccat cctcctacaa taacggttcc gtccggcgcc
     5341 ggcgtggccc gctgcgcgac cgccggcgca gttgtttccc cggtccggcc ccgaccggcg
     5401 cgcagtcctc cgcgaaggcc ccccgcatga cctggttccg caaaaggaag ttcacgacgc
     5461 tgcgtccgcc cagtcagcgc agccggatcc ccgaggggat gtggctcaag tgcagcaact
     5521 gcctggagat catcaccaac aaggactggg aggagaacct gaaggtctgt ccgcgctgcg
     5581 ggcatcatga gcggatcggc gcgcgcgaac ggatccggca actggtcgat cccggctcgt
     5641 ttgaggaata cgacgccggg ctggtctcga ccgatccgct gcaattcgtc gatctgaagc
     5701 cgtatcggga gcatatcgaa gaatcgcggc ggaagtcggg ggagaacgac gcgatcgtct
     5761 gcgggatggg gcggatcgaa acgatgccgc tgtcgctggg ggtgatggaa ttcgccttcc
     5821 gggggggctc gatgggatcg gtggtgggcg agaaggtcgc gcgcacgctg gagcgcgggc
     5881 tggagcaccg cgttccggtg ctgattgtct gcgcctcggg gggcgcgcgg atgcaggaag
     5941 ggatcctctc gctgatgcaa ttggccaaga cgtcggcgct gatcgcgcgg ctgggccagg
     6001 ccggcctgcc gtatttcgtc ctgctcacgc accccagtta cgccgggatc atggcgtcgt
     6061 tcgggtcgct gggcgacgtg attctggccg aaccggaggc gctgatcggg tttgccggac
     6121 cgcgcgtgat cgagcagacg atcaagcaga tcctgccgcc gggcttccag aaatcggagt
     6181 tcgtgcagga acacgggttc gtcgatctgg tgctgccgcg caaggaattg cggccgacgc
     6241 tggcgcggct gatgcggttt ttcggcagcg agccggcgac ggcgagtgag acgcgaccgg
     6301 cggaggccga acgggccagt gagaactccg cgcggattat tgaatcgttc gggccgtagg
     6361 ggcggtgggt ttggggactg gatcggatcg ggatcgcgat cgcaatcggc atcggaagat
     6421 ggttccgcaa ttgccctgac gcacatgaaa ctggatgtct accgcctgtc gattgagtat
     6481 gtagcatggg gctttgccgc agccgaggac ttgacaggga acttttgaca cgcccgagac
     6541 cagtggctga gagccagtca gtccatccca ctcaacgtcg cggaggagaa cggcaaattc
     6601 gccgaagcgg accggcggcg ttcttttgag atcgcgcgag gatcggcctt ggaatgcgcc
     6661 gcgattcggg atgtgctgat ggtggggaat gccatggatg agatggcgca ccgaaaaggc
     6721 aaggaccaac tggaccggat cgtcgcgatg ctgacccgaa tgggcgggcg aagctgcagc
     6781 gtcagggaag agcaggcgag ttacctcgat tcgggatccg atagcgatac cgataccgat
     6841 gcatagcaac accgactcga agggaagaat tttgtgagcg agcagacgcg ttttgccccg
     6901 atggaatacc gccggttcgg caagaccgaa ctcaagctgt cggtcatcac gctgggcggg
     6961 atgcgctacc cgagcagcgg ggcggatccg cagacgaccg agacgctccc cgacgaggcg
     7021 ctggaagcct gctatcagtg cacccaacgg gcgctggatc tgggaatcaa ccatatcgag
     7081 acggcgcacg gctatggcct gagcgagcgg ctctacgggg cgacgtggcg ccggctgcgc
     7141 cagcggcgcg acgagttcta cctgatgacc aagggggcgc cggccacctg ggacgacgcg
     7201 atgcgccagg tcgaggtcca gcttggccgg ctgcagaccg accggatcga cctttacggc
     7261 tggcacggga tcaacacgcc ggagatcaac caatcggcga cggcgaaggg gggaccggtc
     7321 gaggcgctgc accgcctgcg cgaagaggga gtgatcggcc acgtcgggtt ctccacgcat
     7381 ggaccggtgg gggtgattct ggacgcgatc aataccgggc tgttcgagtt cgtcaatctg
     7441 cactgctact atttctttca gcgcaatctg ccggcggtga tccaggccgg cctgcgcgac
     7501 atgggggtct tcatcatctc gcccaacgac aagggggggc agttgttcaa cgcgcccgac
     7561 cggctgcggg ggatctgcgc gccgctgacg ccgatccagt tcaacgcccg cttctgcctg
     7621 tcgcatccgc agattcacac actgagtttc ggcatcgaca agccggagtt ttttgacgag
     7681 gcgatggggg tcctcaacgg cggctactac ctgagccccg cggaccgccg gaccaagctg
     7741 gaactggatc gccagatcga gcgcctgggc gatacctact gcacggggtg ttatgcctgc
     7801 ctcccctgcc ccgaggagat caacatcccc gaggtgctgc gcttccgcaa catgtggaag
     7861 ggttacgaca tggaggcgtt cggccgctac cgttacaaca tgctgcagca gaaggggcac
     7921 tggttccccg gctcgttcgc gtcggcgtgc aacgactgcg gggactgcct gccgcggtgc
     7981 ccggtgcggt tgaacattcc ggagctgctg cgcgagacgc atgcggggct ggcgacgacg
     8041 aactgagggt tgggcaagcg ggatgcgaag tcgcaacagc gtgcaacatg atgcgcaagt
     8101 aaggtttttc ccgacccgtc ggagacggtg gcgaccggcc cttgatcacc tcttccattc
     8161 ctgatttcat ttaacttaaa cgtgtcccga gacccggccc gggcgctggt atgccccttg
     8221 cattcagccc ccggcgacgt tttccaaccc gggaagggat agaagcatga cccatggact
     8281 gaagaagacg gccgccacgg ccgcctgggc gctcctggcg atcgcggtcg cgccctcggc
     8341 gcaggcgctg acgctgcagg gaggctcgct gttcgcaaca gccgacgagg agatcacgat
     8401 cacgctgagc gggatcagca cggcgtatcg cgaccggttg acgctggcgg attcggacga
     8461 cgtgctgttc gattcgcaac agagcgccct cggcgccaac cgcagcctgg gcatgatgcc
     8521 cagcggcggg gaactggtgt tccggctgaa tgtgcgggaa cccgacggcg acagctacgc
     8581 gtggttcagc ggtccggcca gtcgcaacgc cgacaacacg cagcacgcgc ggatcctgga
     8641 tctgggaggc aatcgctggc ggctgtcgtg ggaggatctg tcgctcttcg atccgctgtt
     8701 cgacggcgac ttcaatgact tgatgttcat cgtggagagc cggccgatcc ccgtcatccc
     8761 ggaaccggcg acgctcagcc tggtcggcct ggggctgggc gcgacgggat gggtccgccg
     8821 ccggcggaag cgccgctgac agctgatccg gcaggctttg ggtaagaggc cccgcccgat
     8881 tcccgggcgg ggctttttgc atcgcggggg aagggacttg agggacgaaa gggatgtagg
     8941 gacgcggagt tggggggcgg gggagtgacc ggccagggcg atctcgatcc cggtcccgat
     9001 cccgataccg atctttcagg gccggggaat gttgcaattc aatgcactat cgcctgcaag
     9061 cggttgacac cgcgccgaac gggacgtgaa cggggcaatt ctgtgcgctc actaaattca
     9121 atagacacaa tggaaaacgg tccgaataca aattccccgg atgctggaac agctcttgcc
     9181 atctggtcct gagcgtaagc cgatcctgaa tttaccaggc acggtaagcg gagcgagcaa
     9241 atcgctacca gattccaagg agtcaaagga gccgctatgc gttcccttcc gaaagttctc
     9301 ctggcgctgg cgttgatgtc gggtctggtg ctgccggccc acgccttgac gctgtcgggg
     9361 aattcgctgt ttacctcggg gctgcccgag gtcgtgacgg ttacgtattt aagctcggag
     9421 gcagcttata ccaacctcat gtttgaaacc cagcccaaca cgcagctgct gttcgcgacg
     9481 aacacggcga cctcgggcca gacggtcagt ctgggtgaga tcgcggccaa caccgaactg
     9541 gtgttccggc tcgattcgtt cttcaacggc aaccaggtcg agcaatggta ctcgggaccc
     9601 ggctcgcgca acatcgacgg gacggtccat gcgcgcatca cgttcatcgg caacgatacc
     9661 tggcagttgg cctgggaaga cctcaacttc aacgagtcgg gctatgacgg cgacttcaac
     9721 gacatgatcg tcaacatcaa gagcgcggtg gttcccgagc cggcgacgct ggcgctggtc
     9781 ggactgggcg tgggcagcct ggggctgctg cgccgccgca gccgcaagcc ggccaaggcc
     9841 tgattcacga ctgaccggtt cctccgcccg cgtggccggg gaggatggct tggaccccgg
     9901 agcgacccat gacggatcct ccggggtttt tcttgttcct caagacgtct ctcgcccctc
     9961 gtcctcgtcc tcgactctca aaagcggccg aaacctggga gcgccactct cctgagtggc
    10021 ctcgccccca tcggcctgcc caagattcgg ctccccttat cgtcaccgtg actgagtcga
    10081 gcggttccgc cgtccctgcg ggactcgttc gattcgttgt tcccgtttac ccgccactga
    10141 agtagcgggc tgaagtcagc cgtcgctgac gggactggcc ggcggatcga tccgggatgg
    10201 cctgggccgt aggtttcggt ggccgatcca tcttgtctcc gcttcaccca tttcgccttc
    10261 cgcgcctcgg cgtctccgcg tctcggcgtg agattgcgcc ttttggttca tggccgtccg
    10321 gttcagcgcc gcgcagggat cgtcggccgc cttcccccgc ccggacgcgc caggcctcac
    10381 gccgagacgc ggagacgcgg aggaatttcc caaaggggtc ccgcgcgctg gcgaaacacg
    10441 acggggcgac cactaataag atacgtatat ctactgttgt taatgccgag caggccgccg
    10501 tcctaacctg actcttagga aattgatctc accttgggct ctgcatcacg tttcggtctc
    10561 gcggtcgccg
//
LOCUS       DAOPCO010000134        10533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6137_length_10533_cov_5.909048, whole genome shotgun sequence.
ACCESSION   DAOPCO010000134 DAOPCO010000000
VERSION     DAOPCO010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10533
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_10533_cov_5.909048"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="PLS90_10240"
     CDS             complement(<1..2140)
                     /locus_tag="PLS90_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02821.1"
                     /translation="MSPAPAAGLDPEQRTRRLRGGLLLALLLTPLLIYPEPVLRNEIP
                     LGRDGLAPTLPLHVHAHRLMAQGHEPFWNRCEMAGVPLYAHPRARLLDPLHWPFLIRP
                     SGVTLSLALLLRIGLLGSLTAWLVWRLTGATWIGGWAGAVALCAGWGWHELARGDLSI
                     TLPAAVAWLGAGHAWRGSGRRRWLAAGVLAVAAMTTGEAQLLVAAALAAMLHLGLLLL
                     LDSQGRVRIAMYLVATGAIGVALSAPIWLPRLSYLRFLAGDALFPLPDGSGLAGWHDL
                     SRALAGDWSAAGRAQAPFAGPSPLFTSGLGLAIAAIACVPPGSRRAWRRWLAALAPAA
                     VCLLMAFAPVQAALTESAPRWLPVADAATWLAVGMWYGLLAAAVGLAGVVEGRQPARP
                     RDARPGSLDPLAEEPWAGEQEEFSPGRAFTIPRWARFLPAFGVLMLLTLPQARGIGFG
                     RAAAQALLQFDLALFVLIAALLARRQSAVRTLAALLVVAVLSLGWRTWRSTDPVLLPA
                     EAILTPPPRATEAARLLQPGERLLSFAPGNAATPASRFAWLEPSLAPLWGIEQIGGDR
                     GGASASWREWLGTVLDENPVRGEALDFALPVRAPAYAAANVRAALIARWGFPLNFTQL
                     PGSDFWLAPFPERAEGALRVSFIYRQSGEPTSDPRLVLAHAEGVQWLPLEPSRRLEQR
                     DDVPTTAGRVDAPIAPDWRIATGEVDVDEVR"
     gene            complement(2137..2547)
                     /locus_tag="PLS90_10245"
     CDS             complement(2137..2547)
                     /locus_tag="PLS90_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02822.1"
                     /translation="MDSLPEISPHTLAHGDAVGHPKRFRRFRPRRVSDTMDDMPHCAY
                     CHQPIPANVHPYTLRLELFPAVEPSLQIREEDLKLDFEAEMRRLIRIMEQMDEAQVVE
                     QEQRMFLSRSFTLCPACRDRLARQLESLKPGEPA"
     gene            3254..4132
                     /gene="lipA"
                     /locus_tag="PLS90_10250"
     CDS             3254..4132
                     /gene="lipA"
                     /locus_tag="PLS90_10250"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPK02823.1"
                     /translation="MVPQRAELKTSKPPWLRARLPTAPGTEQVHQVMRTQRLHTVCES
                     AHCPNMGECWSHGTATFMINGNVCTRSCSFCAVFTGRPLPLDPDEPRRVAAAAEAMRL
                     SYVVVTAVNRDERADGGAAQFAATIRALREQRPGIRVEVLIPDFKGDADALETVFRER
                     PDVLNHNTETVPRLYRRVRPQARYERTLDVLRRAKAAGLVTKSGIMLGLGEEDDEVRR
                     VLHDLRAHGCDIVTIGQYLRPSEKHHPVVRYVSPTEFAAWKRYGEDLGFTTVESGPLV
                     RSSYHAHESFGRHQRG"
     gene            complement(4148..4567)
                     /locus_tag="PLS90_10255"
     CDS             complement(4148..4567)
                     /locus_tag="PLS90_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPK02824.1"
                     /translation="MKTLREELWFNVPTRRAFINITQQVQDLVERSGVRDGLVLVNAM
                     HITASVFINDDESGLHQDYEEWLERLAPFDPSTRYQHNRTGEDNADAHLKRQIMGREV
                     VVAITDGRLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(4619..5365)
                     /locus_tag="PLS90_10260"
     CDS             complement(4619..5365)
                     /locus_tag="PLS90_10260"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPK02825.1"
                     /translation="MTGRKRERSEAEAAAASNADEVRRLLEREGAVAVDTAEAWTEPP
                     DELEEEVREWGCGWLLLLILLGLTSLFAVGWWGVNEITRRNMVEKLDKSFFHLSLLPQ
                     PKVETAEPDLTVQIFYLVRGRGLAAQKRELRYHADSRERLYQIAHEMQRPPTTGLLES
                     PFPKGTEIRAIYLHDGIIWIDLNEAFLQPEHPSPLLERLVIFAIVNNFMLNEPTYRGV
                     RFMIEGQPVNSAWGWLDLSSPLGPDLSLIE"
     gene            complement(5362..6408)
                     /locus_tag="PLS90_10265"
     CDS             complement(5362..6408)
                     /locus_tag="PLS90_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPK02826.1"
                     /translation="MTTEYTVKLPIFEGPLDLLLHLIKMNEMEITEITISAIIGQYLE
                     YLRMMESLDLEIAGDFLVMAATLLNIKLRTLLPPPREGEEEQGEEELNDFLNARQLMQ
                     RLIEYRKFKEAALELGRRHEKQAQIFLREVALPRLTEAEENAEIRGDLHKLLEAFSRV
                     IRFVDRRDYHQVTEEEYSVDDKMTLLRRRLVVEPRMELNRLFESCDEKIEMVVTLIAV
                     LELTRLKELRIVQGENFGEVYIFRREGDEGNADQEGEAAELQRVEAEILANRTGLVDD
                     GVLDEEDEDAELDRVLGTAADADERPRPGEAEPQTEAAPESDEPGGARIIDLGFEPRA
                     GDDGADPEGRDPAP"
     gene            complement(6590..7492)
                     /gene="kdsA"
                     /locus_tag="PLS90_10270"
     CDS             complement(6590..7492)
                     /gene="kdsA"
                     /locus_tag="PLS90_10270"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPK02827.1"
                     /translation="MRGPHGTAGGEPVSAQESRQPLVTTLPDPAASIDPFSAGRDRLL
                     LIAGPCVIESEEHTLRMAEAIKAIAGRAGFPCIFKASYDKANRTSLASYRGPGLDAGL
                     RVLKRVRLDVGLPVLTDFHEPGQAAAVAEAADVLQVPAFLSRQTDLIVAAARTGRIVN
                     IKKAQFMAPWDIEFSINKAREAGAERIWVTERGVCFGYNNLVSDMRSLVVMARYGCPV
                     IYDATHSIQIPSGAGGKSGGERQYLEPLLRAAVAVGVDGVFMEVHDDPDRALCDGPNQ
                     LPLDRLPDVLETIRRIDAARRGET"
     gene            7611..7889
                     /locus_tag="PLS90_10275"
     CDS             7611..7889
                     /locus_tag="PLS90_10275"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPK02828.1"
                     /translation="MGGEFDVISKDLLEILVCPESHQTLTPADAATVARLERRRAEGT
                     LKNRAGKPVADPIQHALIREDRRIAYPIVDSIPIMLIDEGIPLDQLDS"
     gene            7942..8538
                     /locus_tag="PLS90_10280"
     CDS             7942..8538
                     /locus_tag="PLS90_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02829.1"
                     /translation="MFGWLNQFWMNWLLAGRRAEARDGGAGHWKLDTVGRYRFSSLLF
                     MGLWVVLAAGVTLDAWLRPEDATRRREEAAAWFSLFVVPLAMTLFGIVDAWWARVTIE
                     PAGLRFQYPWRPGALVRWEDIVQIRHRESFHWFTIRRRGGAAIRIAETRHGLGRLRRE
                     LRRHVDPLRWAAAAARLPRLDADDGVGGSEAEAGARAG"
     gene            complement(8592..8744)
                     /locus_tag="PLS90_10285"
     CDS             complement(8592..8744)
                     /locus_tag="PLS90_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008105479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02830.1"
                     /translation="MTLGGWIFMLLSVGGIFVWTVYCFVRVFRARDAREHMHGPLDID
                     THDRGT"
     gene            complement(8741..10369)
                     /locus_tag="PLS90_10290"
     CDS             complement(8741..10369)
                     /locus_tag="PLS90_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPK02831.1"
                     /translation="MADLNPSAQTLERRRENWGTKLGVILAVSGSAVGLGNFLRFPGL
                     AATHGGGMFMIPYFISLLLLGIPLVWAEWAMGRYGGARGYNSAPGIYSVIWPRPWSRY
                     FGALALMVPIVIYMYYVYIESWCLYYAWSYLRAFVTGNRGVSPGESVEQGAAFFSNYV
                     QDTQNGLPGPAIAMLILVFLLNYVFIYRGVTRGIETFSRYAMPLLVLCALVVLVRVLT
                     LPNILDGLGFMWNPSLPEGQSTWDYLKNAEVWLLAAGQIFFSISVGFGIIINYASYLR
                     RNDDVVLSGMTACATNEFCEVCLGGLITIPVAFIFLGGLPEGLLRSISEGSSLAVGFF
                     ALPPVFGLMPLGLLFGALWFFMLSLAAITSSLSMLQPTIAFLEEGMGLGRRAAVALLG
                     LLTAIGCLIAVYFSKNLAALDTMDFWVGSVCIYVLATIQVLMFGWAFGIERARAEIAQ
                     GAEMKVPAIFWPVIRYVTPVYLLVIFLFWAKQKFPERIRGIVELDAADRAGVLFPLIY
                     IGLLILFFVIMIALTRQRMDRLRVEEHAREEVVP"
BASE COUNT         1772 a         3765 c         3431 g         1565 t
ORIGIN      
        1 cgcgcacctc gtcaacatcg acctcccccg tggcaatgcg ccagtccggc gcgatcggtg
       61 cgtccacccg gcccgccgtg gtgggcacgt cgtcgcgctg ctccaaccga cgcgaaggtt
      121 ccagcgggag ccactggacg ccctcggcgt gcgccagcac cagccgcgga tcgctcgtcg
      181 gctccccgga ctgacgataa atgaagctga cgcgcaacgc cccctcggcg cgctcgggga
      241 acggggcgag ccagaaatcg gaaccgggca gctgcgtgaa attgagcgga aatccccacc
      301 gcgcaatcag cgccgcgcgc acgttcgccg cggcataggc cggcgcccgc accggcagcg
      361 cgaagtccag cgcttcgccg cggaccggat tctcatccag aaccgttccg agccactcgc
      421 gccagcttgc cgaggccccg ccgcgatcgc ccccgatctg ctcaatcccc cacagcggcg
      481 ccagcgatgg ttccagccag gcgaaccggc tggccggcgt ggcggcgttg cccggcgcga
      541 acgagagcag gcgctcgccc ggttgcagca gccgcgccgc ttcggtcgcc cgcggcggcg
      601 gcgtcaggat cgcctcggcg ggcaacagca ccgggtccgt cgagcgccag gtccgccagc
      661 ccaggctcag cacggcgaca acgagcagcg cggccagcgt gcgcacggcc gattgcctcc
      721 gcgcgagcag cgcggcaatc agtacgaaga gcgccagatc gaactggagg agggcctggg
      781 cggcggcccg cccgaagccg atcccccgcg cctgcggcag cgtcaggagc atcagcacgc
      841 cgaacgccgg gagaaaacgt gcccagcgcg ggatcgtgaa cgcccgtccc ggcgaaaatt
      901 cctcctgctc gccggcccac ggttcctccg ccaacggatc cagcgacccc ggccgggcgt
      961 cccgcggccg cgcgggctgg cgcccctcca cgacgccggc cagaccgact gcggcggcca
     1021 gcaacccata ccacatcccg acggcaagcc aggtcgccgc gtccgccacc ggcagccagc
     1081 gcggcgccga ttcggtcagg gcggcttgca ccggggcgaa cgccatcagc aggcagaccg
     1141 ccgccggcgc cagcgccgcc agccacctcc gccacgcgcg gcgcgagccc ggcggcacgc
     1201 aggcgatcgc cgcaatggcg agcccgaggc cgctggtgaa gagcggcgaa ggaccagcaa
     1261 acggcgcctg cgcccggccg gccgccgacc agtcgccggc aagcgcccgg ctcaggtcgt
     1321 gccagcccgc cagaccgctg ccgtccggca acgggaagag cgcgtccccc gccaggaacc
     1381 gaaggtaact gagccgcggc agccagatgg gggcgctcag ggcgacgccg atcgcaccgg
     1441 tcgccaccag atacatggcg atccgaacgc ggccctgcga atcgagcaac agcagcagac
     1501 cgaggtggag catcgccgcg agcgccgcgg ccaccaggag ctgcgcttcg cccgtcgtca
     1561 tcgcggcgac ggccagcacc cccgcggcca accagcggcg ccggcccgag ccgcgccagg
     1621 cgtggcccgc ccccagccag gccaccgcgg cggggagcgt gatggacagg tcccctcgcg
     1681 ccagctcgtg ccagccccac ccggcgcaga gcgcgaccgc cccggcccag ccgccgatcc
     1741 acgtcgcgcc cgtgagccgc cagaccagcc acgcggtcag gctgccgagc agaccgatcc
     1801 gcagcagcag cgccagcgag agcgtcacgc cggagggtcg gatcaggaac ggccagtgca
     1861 gcggatcgag cagccgcgcc cgcggatggg catagagcgg aacgcccgcc atctcgcagc
     1921 ggttccagaa cggctcgtgg ccctgcgcca tcaaccggtg cgcatgaaca tgcagcggga
     1981 gggtcggcgc caggccgtcg cgccccagcg ggatctcgtt gcgcagcacc ggctcgggat
     2041 aaatcagcag cggcgtcagc agcagcgcga gcagcagccc cccgcgcagg cggcgcgtgc
     2101 gctgttccgg atccaatccg gccgcggggg ccgggctcat gccggctcgc ccggcttgag
     2161 ggattccagt tgccgcgcca gccggtcgcg gcaggccggg cagagcgtga agctgcgcga
     2221 gaggaacatg cgctgctcct gctccacgac ctgcgcctcg tccatctgtt ccatgatgcg
     2281 gatcagacgg cgcatctccg cctcgaaatc gagcttgagg tcctcctcgc ggatctggag
     2341 cgacggttca acggccggaa agagctccag ccgcaacgtg tagggatgaa cattggccgg
     2401 gattggctgg tggcaatagg cgcagtgcgg catgtcgtcc atcgtatcgg aaacccgccg
     2461 cgggcgaaac cggcgaaacc gcttcggatg gccgaccgcg tcgccgtgtg ctaaagtatg
     2521 gggcgagatc tccgggagcg agtccatgaa cgcacgcctg ctggcccaga tcgacaccac
     2581 cccgccgccg gctcccgccg acggtccgtg gccgaccgag gtcctgttct tcacgcagat
     2641 cccctgcgag gacgggctgc gcctgcaggc cgacggcttc gaaaagatcg ccgcaggatc
     2701 ggcgccgggc cacctgttgg tcctgaccga gcatccaccc acgtggctgg cgcccacgga
     2761 ggacgcagcc cggcggctct gcgacgccgc ccccgccgag atcgccgtcc tgcgcgacga
     2821 gcaggcccgc gcggccgagt ggaccgggcc gggcgtgctg acgacctacc tggtctatca
     2881 tctggatcac ggccccgagg agcagcccgt gccgcgcgac gccgacgcct tcgtgacgcg
     2941 cgtcgaggac ctgctcctgg ccgtcctgcg cgactttggc gtcgaaggcg agcgccagac
     3001 cgacggccgg tccatccgcg tcggcggccg cacggtcgcg accatccgca cccgcgtgcg
     3061 ccgctggatc gccctcgtcc ggttcaccat caataccaac gccgacccgg tcctcgccgc
     3121 ggggctgcgc cgcgccggat tcgactcgcc cccgaccgcg agcctgaccg atctggggct
     3181 ggatgtcgag cgccaggacg acgtacgcga ggcggtcgtg cggcactacg aggccctctt
     3241 ccgcgcgcgg acgatcgtcc cccagcgcgc cgagctgaag acgagcaagc ccccctggct
     3301 gcgtgcccgc ctgcccaccg cccccggcac cgagcaggtc caccaggtaa tgcgcacgca
     3361 acggctgcac acggtctgcg aaagcgcgca ttgccccaat atgggcgagt gctggagcca
     3421 cggcaccgcc acgttcatga tcaacggcaa cgtctgcacg cgctcctgca gcttctgcgc
     3481 ggtcttcacg ggtcggccgc tgccgctcga ccccgacgaa ccgcggcgcg tggccgcggc
     3541 ggccgaggcg atgcgcctgt catacgtggt cgtcacggcg gtcaatcgcg acgagcgcgc
     3601 cgacggcggc gccgcccagt tcgccgccac catccgcgcc ctgcgtgagc agcgccccgg
     3661 cattcgcgtc gaagtgctga tccccgattt caagggcgac gccgacgcgc tggagaccgt
     3721 cttccgcgaa cggcccgacg tgctcaatca caataccgaa acggtccccc ggctttaccg
     3781 gcgcgtgcgc ccgcaggcgc gctacgagcg cacgctcgac gtgctgcggc gcgccaaggc
     3841 ggccggtctc gtcaccaaga gcggcatcat gctgggtctg ggcgaggagg acgacgaggt
     3901 gcgccgggtc ctgcacgacc tgcgcgccca cggctgcgac atcgtcacaa tcggccagta
     3961 cctgcgcccc tccgagaagc atcatccggt cgtccgctac gtcagcccga ccgaattcgc
     4021 cgcctggaag cgctacggtg aagacctcgg cttcaccacc gtcgaatccg gcccgctggt
     4081 gcgcagttcg tatcacgccc acgagagctt cggccggcac cagcgcggtt gagcgccggc
     4141 cgccgcgcta ctcgccgatg atcttgacca gcacgcgctt gcgccgccgg ccatcgaatt
     4201 caccgtagaa aatctgttcc cacgggccga agtcgagccg cccgtcggtg atcgcgacga
     4261 ccacctcgcg ccccatgatc tggcgcttca ggtgggcgtc ggcgttatcc tcccccgtcc
     4321 ggttgtgctg atagcgcgtc gacgggtcga acggggccag cctttcgagc cattcctcgt
     4381 agtcctgatg cagccccgat tcatcgtcgt tgatgaagac gctggcggtg atgtgcatcg
     4441 cgttgaccag cacgagcccg tcgcgcacgc cgctgcgttc gaccagatcc tgcacctgct
     4501 gcgtgatgtt gatgaacgcg cggcgcgtcg gcacgttgaa ccagagctct tcgcgcaagg
     4561 ttttcatgag gttcacctca gcccatgaag tcggaacgag tcgcgcggcg gggcgcgcct
     4621 attcgatcag cgacaggtcg ggacccagcg gactgctcag gtcgagccag ccccacgccg
     4681 agttgaccgg ctgcccctcg atcatgaacc gcaccccgcg atacgtcggc tcgttcagca
     4741 tgaaattgtt gacgatggcg aagatcacca gccgctccag cagcggcgag ggatgctccg
     4801 gctggagaaa cgcctcgttc aggtcaatcc agataatgcc gtcgtgcaga tagatcgcgc
     4861 gaatctcggt tcccttcggg aaaggcgatt cgagcagccc ggtcgtcggc ggccgctgca
     4921 tctcgtgcgc gatctgatac agccgttcgc ggctgtcggc gtggtagcgc agttcgcgct
     4981 tctgcgcggc gagcccccgg ccgcggacca gatagaaaat ctgcacggtc aggtccggtt
     5041 cggcggtctc caccttgggc tgggggagga gcgacagatg aaagaacgac ttgtcgagct
     5101 tctccaccat gttgcgccgc gtgatttcgt tgacgcccca ccagccgacc gcgaagagcg
     5161 aggtcagccc cagcaggatc agcagcagca gccagccgca cccccattcg cggacttcct
     5221 cctcgagttc gtccggcggt tcggtccacg cctcggccgt atcgacggcg acggccccct
     5281 cgcgttcgag cagccggcgc acctcgtcgg cgttcgatgc cgcggcggct tccgcttcgc
     5341 ttcgttcgcg cttgcgtccg gtcatggtgc gggatcccga ccctccgggt ccgcgccatc
     5401 gtcgccggcg cgcggctcaa agccaaggtc gataatcctt gcgccgcccg gttcatccga
     5461 ttccggcgcg gcctcggtct ggggctcggc ctcgccgggc cgggggcgct cgtccgcgtc
     5521 cgccgcagtg ccgagcacgc ggtccaactc ggcgtcctcg tcctcctcgt ccagcacgcc
     5581 gtcatccacc agcccggtgc ggttggcgag gatctcggcc tcgacgcgtt gcagctcggc
     5641 cgcctcgccc tcctggtcgg cgttcccctc gtccccctcg cggcggaaaa tatagacttc
     5701 gccgaagttc tccccctgga cgattcgcag ctccttcagc cgcgtcagtt ccagcacggc
     5761 gatcagcgtg acgaccattt cgatcttttc gtcgcagctc tcgaacagcc ggttcagctc
     5821 catccgcggc tcgacgacaa gccgccgccg caacagcgtc atcttgtcgt cgaccgagta
     5881 ttcctcctcg gtcacctggt gatagtcgcg ccggtcgacg aagcggatga cgcgcgagaa
     5941 ggcctcgagc agcttgtgca gatcgccgcg gatctcggca ttttcctcgg cctcggtcag
     6001 tctgggcagc gccacctcgc gcaggaagat ctgtgcctgt ttctcgtggc ggcgccccag
     6061 ctccagcgcg gcttccttga atttgcggta ttcgatcaac cgctgcatga gctgacgcgc
     6121 gttgaggaaa tcgttcagtt cctcctcgcc ctgttcctct tcgccctcgc gcggcggggg
     6181 gagcagcgtg cgcagcttga tgttgagcag cgtggcggcc atcaccagaa aatcgccggc
     6241 gatctccaga tcgagcgact ccatcatccg caggtattcg agatactggc cgatgatcgc
     6301 cgagatggtg atctcggtga tctccatctc gttcatcttg atcaggtgca gcagcagatc
     6361 gagcggcccc tcgaagatcg gcagtttaac ggtatactcg gtggtcatct cgcccgtgcc
     6421 tgcatcgccc gattcatcaa agttcttcgt ctctcgtctc tcgccctcgg cccccgaagg
     6481 tctccgcaaa attcggctct cggctctcga acggacttca gcggcatccg ggcttcaccc
     6541 gtttcgtgcc ctgcgtgtct ttcgggatcc agcctcgccg tatttccctc tacgtttccc
     6601 cccgtcgcgc cgcgtcgatc cgccggatcg tctccagcac atcgggcagc cggtccagcg
     6661 gaagctgatt cggcccgtca cagagcgcgc ggtcgggatc gtcgtgcact tccatgaaga
     6721 cgccgtcgac gccgacggcg acggcggcgc gcagcaacgg ttccagatac tgccgttcgc
     6781 cgccgctttt gccccccgcg ccgctgggga tctggatgct gtgcgtcgcg tcgtaaatca
     6841 ccgggcagcc gtagcgcgcc atcacgacca gcgaacgcat gtcgctgacc agattgttat
     6901 aaccgaaaca gacgccgcgc tcggtgaccc agatccgttc ggcccccgcc tcgcgcgcct
     6961 tattgatcga aaactcgatg tcccacggcg ccatgaactg ggccttcttg atattgacga
     7021 tccggccggt gcgggccgcg gcgacgatca ggtcggtctg gcggctgagg aacgccggca
     7081 cctgcaggac gtcggccgcc tcggcgaccg ccgccgcttg gcccggctca tgaaaatcgg
     7141 tcagcaccgg caggccgaca tcgagccgca cgcgcttcag gacgcgcagg ccggcatcca
     7201 gccccggacc gcgataggac gccagcgacg tgcggttggc cttgtcgtac gacgccttga
     7261 agatgcaggg gaaccccgcg cgcccggcga tcgccttgat cgcttcggcc atgcgcagcg
     7321 tatgttcctc cgactcgatc acgcaggggc cggcaatcag cagcaggcgg tcgcggccgg
     7381 cggagaatgg gtcaatcgag gcggcgggat cgggcagcgt cgtcacgagg ggctgtcggc
     7441 tttcctgggc tgagaccggc tcgccgccgg cggttccatg cgggcccctc atcttcgcca
     7501 aagcgccggc ccgtttgaag atttaattgc aaagcccgcc gccggctccg aagatgaaac
     7561 cgacgccgac cggcgcggcc gggggcattt ccccaacgga gttttcaacc atgggcggcg
     7621 aattcgacgt catttccaag gatctgctcg agatcctcgt ctgccccgaa tcgcaccaga
     7681 ccctgacgcc ggccgatgcg gccacggtcg cccggctgga acggaggcgc gccgaaggca
     7741 cgctcaagaa ccgggcgggc aagccggtcg ccgatccgat tcagcatgcc ctgatccgcg
     7801 aggaccgccg gatcgcttac cccatcgtcg attcgatccc aatcatgctg atcgacgaag
     7861 gaatcccgct cgatcagctc gattcctgac ggcgcgtccc ggcgcttttc ccaacctgct
     7921 ccatgatcgg gaggagctcc gatgttcggc tggctcaacc agttctggat gaactggctg
     7981 ctggccggac ggcgggcgga ggcgcgcgat ggcggcgccg gccactggaa gctggacacc
     8041 gtcggacgct accggttctc cagcctgctg ttcatgggcc tgtgggttgt gctggcggcg
     8101 ggcgtgacgc tggatgcctg gctgcgcccc gaggacgcga cgcgccggcg cgaggaagcc
     8161 gccgcctggt tcagtctgtt cgtcgtgccg ctggcgatga cgctgttcgg catcgtggat
     8221 gcctggtggg cgcgggtgac gatcgagccg gcgggcctgc gctttcaata cccgtggcgc
     8281 ccgggcgcgc tcgtccgctg ggaggacatc gtccagattc gccaccgcga gtcgttccac
     8341 tggttcacga tccgccgccg gggcggcgcg gcgatccgca tcgccgagac gcgccacggg
     8401 ctggggcggc tgcggcgcga actgcgccgc catgtcgatc cgctgcgctg ggccgccgcc
     8461 gccgcgcgcc tcccccggct cgatgccgac gacggggtgg gcgggtccga ggccgaagcc
     8521 ggagcgcggg cgggatagcg attgcgagac cgattgccac accgattggg actgcgctac
     8581 cgatagcgat ttcaggtgcc gcggtcgtgc gtgtcgatat cgagcgggcc gtgcatgtgc
     8641 tcgcgcgcgt cgcgggcacg gaagacgcgg acaaagcagt aaacggtcca gacgaaaatt
     8701 ccgcccacgc tgagcaacat gaaaatccag ccgccgaggg tcatgggacc acctcctcgc
     8761 gcgcgtgctc ctcaacgcgc agacggtcca tccgctggcg cgtcagcgcg atcatgatga
     8821 cgaaaaagag gatcagcagg ccgatgtaga tcagcgggaa aaggacgccg gcccggtccg
     8881 cggcatccag ttccacaatg ccccgaatgc gttcggggaa cttctgtttc gcccagaaaa
     8941 ggaaaatcac cagcagatag accggcgtca cgtagcggat caccggccag aagatcgcgg
     9001 ggactttcat ttcggcgccc tgcgcgattt ccgcccgggc ccgctcgatg ccgaacgccc
     9061 agccgaacat caacacctga atcgtcgcca ggacgtagat gcagacgctg ccgacccaga
     9121 aatccatcgt atcgagcgcc gccagatttt tggagaaata caccgcgatc aagcagccga
     9181 tggccgtcaa caggccgagc aacgccaccg ccgcccgccg gcccaggccc atcccttcct
     9241 ccagaaacgc gatcgtcggc tggagcatgc tgagcgaact ggtgatcgcg gccagagaca
     9301 gcatgaagaa ccagagggcg ccgaacagca ggcccagcgg catcagcccg aacaccggag
     9361 ggagcgcgaa gaatcccacg gccagactcg acccctcgct gatcgacctg agcagcccct
     9421 cgggcagccc gcccagaaaa atgaacgcca ccgggatcgt gatcagcccg cccaggcaga
     9481 cttcgcagaa ttcgttggtc gcgcaggcgg tcatgcccga cagcacgaca tcgtcgttgc
     9541 gccgcaggta gctggcgtaa ttgatgatga tcccgaaccc gaccgaaatg ctgaagaaga
     9601 tctgcccggc cgccagcagc cagacctcgg cgttcttcag atagtcccac gtgctttgcc
     9661 cctcgggcag cgaggggttc cacatgaagc ccagcccgtc cagaatattg ggcagcgtga
     9721 gcacgcggac cagcacgacg agcgcgcaca ggacgagcag cggcatggca tagcgcgaga
     9781 acgtttcgat gccgcgcgtc acgccgcggt agatgaatac gtaattgagc aggaagacga
     9841 gaatcagcat cgcgatcgcc gggccgggca ggccattctg cgtgtcctgg acatagttgg
     9901 agaagaaggc cgccccctgc tcgacgctct cgccgggact gacgccgcga ttgcccgtca
     9961 cgaacgcccg cagatacgac caggcgtagt acaggcacca cgattcgatg tagacgtaat
    10021 acatgtagat gacgatcggc accatcagcg ccagggcgcc gaaatagcgg ctccacggcc
    10081 ggggccagat gacgctgtag atgccgggcg ccgagttgta gccccgcgcg ccgccgtagc
    10141 gccccatcgc ccactcggcc cagaccagcg ggatgcccag cagcagcagc gagatgaaat
    10201 acgggatcat gaacatcccg ccgccgtggg tcgccgccag gccgggaaag cgcagaaagt
    10261 tgcccaggcc gacggccgag cccgagacgg cgaggatcac gccgagtttg gttccccagt
    10321 tttcacgccg acgttccaac gtctgggcgg aagggttgag atcggccatc ggtcatcctc
    10381 aacgccgtcg cgtgcggctc gcgcctcggc ccggcgggcc cggccgacgg cgcggaacgt
    10441 tgcgatcgca gaggtttgga attgccgcgg cgcggctacg gcttagcagt ttgcgggatc
    10501 gaatcggcgc ctgcaactga gaaagccgcc cgc
//
LOCUS       DAOPCO010000135        10510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6150_length_10510_cov_5.404687, whole genome shotgun sequence.
ACCESSION   DAOPCO010000135 DAOPCO010000000
VERSION     DAOPCO010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6150_length_10510_cov_5.404687"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..1270
                     /locus_tag="PLS90_10295"
     CDS             335..1270
                     /locus_tag="PLS90_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPK02832.1"
                     /translation="MLLPAMVALAACGEPETVSSAGERESAGRAAGPIRVQVAIAPIA
                     TLVERVGGELVEVGVLTDAGQNPEQFQPTARQITELSAADLYLTLELPFERNLTERLA
                     PPPAGPRIVSLVEGLALRTMPGHFHAGEDDATTAPPGAAAHEYAGAADPHVWLDPVLT
                     AQMAQRAAEALSLAAPDRAETFSQNAAAARAELERVDAELRELLGRARGRAFMIYHPA
                     LGYLADRYGLRQLPIEVQGKEPAARELSRTIERARAESIRTIFIERQFDKRNAMAIAQ
                     AIGAAVEEIDPLAPDYMENMRRIGQALAAALKPVE"
     gene            complement(1293..1781)
                     /locus_tag="PLS90_10300"
     CDS             complement(1293..1781)
                     /locus_tag="PLS90_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02833.1"
                     /translation="MGAIIALTAALGWSWPALLPIVGAVAAAKGYQLLSDPRSPAFQS
                     RLRRELDARRIERVPLDSELAEIISDEIGKEERLIFEREDFVLVFRKDARGKFFIDAM
                     GPRNKTRLDLRLRADEFAREIVRKFAYHRLAEQLLRVNAVIVEEQVDDAGRITMTARR
                     WN"
     gene            complement(1885..3771)
                     /locus_tag="PLS90_10305"
     CDS             complement(1885..3771)
                     /locus_tag="PLS90_10305"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HPK02834.1"
                     /translation="MSVTPPPPSLRNHPFMRALAERAVLFDGGMGTMLYQRGVFITQC
                     FEQQNLLKPDLVRELHAAYVRSGAEVLETNTFGANRIKLARHGIEPDEMRAIIQAGVR
                     LARDAGGQKIWVAGSVGPSGRHFGAPGDDGIDPARAHDLFLEHTGLLAEAGVDLLVLE
                     TFREMRELEAAVAAAREAAPHLPIVALLTPDAEGNTADGLDLEQVARRLEALAPAAGG
                     FNDGAGPDGMFHLMRRMAKAAPGLTLAAQPFIGGVETIEDRTIHLATTEFTGVYARRM
                     FREGVRVLGLCCGATPAHIKAMRNALSALQPGAQKSAGIQMVGPSEAEPVEQANRPVA
                     TRFARKVAAGEFVVSVEIDPPQGTDISKALESARKLRDSGRVDVINIADGPRALARIN
                     PSSMALILKRDFDMEAIIHYCCRDRNLLAMQADLLAANALGLHNILLITGDPPKMGDY
                     PDATAVFDVDAIGLTRIVTRLNQGRDLAGNSIGEPTRFFQGVGANPGAQDINIEIERL
                     QRKRDAGARYVMTQPVYNAEVFERFIERAAPLGLPILIGILPLRSLRNALFLHNNVPG
                     MAIPAEVLARMEAAPEGADQRRAGWEIAREALRRALPYVQGTYIMPPFGSAEDALAVL
                     EALD"
     gene            3916..5724
                     /locus_tag="PLS90_10310"
     CDS             3916..5724
                     /locus_tag="PLS90_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02835.1"
                     /translation="MFRRFDRLKPYLWRHRNPLLLGMACLVASNYLDIRIAVMLGAGI
                     DAVGLPAGPFGAVKAELLTIFLLVTLVLGIGAGVTRFYMRRLIINTSRNVEYEFRNDL
                     FGHLLRLPASFYDRFRTGDVMARATSDIEAIRMVLGPAIMYVANSLVLFPMAVWQMAV
                     ISMRLMLLCFVPMLLVIPVVYYFRSRINIRARLVQEANSDLSANVQENLSGMRVIKVY
                     AREQTQAEAFDRLSQRYVKANMDLVRLQAVFFPALSLMIGGVVAMLLLGGGVMIIRGT
                     LTLGAIITFFALLMTCIWPLAAFGWIMAQLERGAASMKRVNEIFEAAPEASDEPEAAA
                     EDLAAGAPAIAPVMRGEIRLRDLTFRYPGSERPALAGIDLEIPAGGTLGLTGPVGCGK
                     STLAHLLMRRYDPPRGALFIDGRDILDWPIAALRRQIGIVDQEPFLFSDTIAANVAFG
                     VAEQEAERLAPRIERVTRVAQLDDEVRGFPQGYETILGERGINLSGGQRQRSALARAL
                     ARDPALLILDDALAAVDTHTEEAILRGLRELLGQRTTLIISHRISTVSLADRIVYLEA
                     GRVAEQGTHEELLARRGKYWMLAQRQRLAEEIERTA"
     gene            5743..7617
                     /locus_tag="PLS90_10315"
     CDS             5743..7617
                     /locus_tag="PLS90_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02836.1"
                     /translation="MSKLKQALAPDEALAYRRRIDTRLLGRLWEFVRPYQGIVGFTVL
                     LSLAANALRVLQPLVGMYIIDHQISEGDVNGLILMVLLFIGLLLLASACDVAFTWFIS
                     WVGQRAMDTMRRRLIRHIFAQDTVFFDRNPVGRLITRLTSDVETLNELFASGLVAVMG
                     ELLIIIGVIALMLTQSAKLTGVVLLSAPFILAVAIFFRRHSRKWYLETRRALATMNAY
                     LQENVAGMRTVQAFNRQRRNHRQFASLNNEYRVANLHTIFAFALFFPAMSFISSMAVA
                     AVIWVGGRDMIAGRGAAAGLSFGQLFFFVQCVHMLFAPVRSLSEKYNLLQAAMASSER
                     IFKLFDQRPTIDAPREPAPIRPLTDAIRFEDVRFAYVPDEPVLKGISFEIRRGQSVAV
                     VGATGAGKTTLINLLTRFYDVTSGRITYDGVDLRRFDPRALRRQFAVVLQEVFLFSGT
                     IADNLRLANPELTEEEMWEVLRQVRAEDFVASLAGGLHAEVSERGGTFSTGQKQLLAF
                     ARALASDPQLLILDEATANIDTETEQRIQEAIGTVLAGRTALVIAHRLSTIQRADRIL
                     VMHHGQIHEAGTHAELLAIDGLYRRLYELQYRHEAVADGGDPAAPAAAEQLTNESQAL
                     "
     gene            7614..8660
                     /locus_tag="PLS90_10320"
     CDS             7614..8660
                     /locus_tag="PLS90_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPK02837.1"
                     /translation="MTASRASYLMEPRRIEFQDEARPAPGPGEVLIELTAVGVCGSDV
                     HWYLDGRIGTTRLEGPLTLGHEPAGRVVEVGAGADPALKGKRVAIEPAIACGHCPFCQ
                     DGDTNLCPHVRFMGTPPTQGAFRQWTTHPAHLVAELPATVSDEVGALLEPLAIAIHAT
                     DLLKPRAGATWAIVGAGPIGLCALEAVKLTGPGCVMIVEPLEYRRALARSMGADVVLA
                     PEQDDLAAAVARATGGYGADYVIEAAGKPASFELMTQVARPGAKIAVVGIDPHDRFGF
                     NNSVGRRKGLTLYMVRRSRRTLERAIAITAAGRWRPDPIITHRRPFGDLARSLERVAG
                     FEDGVIKTIIDPRR"
     gene            8797..9873
                     /locus_tag="PLS90_10325"
     CDS             8797..9873
                     /locus_tag="PLS90_10325"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPK02838.1"
                     /translation="MKLALNALALQLIQMSPADEPPEAGAPEPGGRPEPPRSTRPDRG
                     RQRSGAPETPAALSWQARPGWRGRALAAWIQFDVVDLPWLQMAYEGRRRDGLTCVKLS
                     RSRHVLRVRRPERFGARDPRGVYVKRYLINTPRRRLGNLLSGGKARREFRLGWRLIQR
                     GLRTARPLAYVIADPMRILDRQAPPGFARAASFLVTEEVLNDGTIYEWACRGDAAGCE
                     GFYPALARYIAAMHQRGFYHDDLSGKNICVASGEELRAWLAADRGAATAAANGLLSKF
                     ILFDVDHGRLFREGKVPFRLRMRNVFQILRSLRHFDMKTRAARRQFVEDYLEAAGLST
                     ARRRRAAEKSINRFARARLGHELV"
BASE COUNT         1577 a         3218 c         3985 g         1730 t
ORIGIN      
        1 cgagggacga gagccgagag ccgaattttg gagcgggcct ttgggggcga tgacggagac
       61 aaggacgagg acgagggacg agagccgaga gccgaatttt ggagcgggcc tttgggggcg
      121 atgacgaaga caaggacgag gacgagggac gaagatgagg gacgaattgg atgagagtag
      181 ggggcgtgat atgctcaagc gtgctggcgt ttcgccgacg gtggcgcgcg cccacacctt
      241 gcgcaagaaa ggtcggttgt cgctgatgtc ccttgcgtcc ctgaaatccg taacatcccg
      301 agcgcgcagg tccttgccgg gccgggcatt ggccgtgctg ctgccggcca tggtggcgct
      361 ggcggcgtgc ggcgagccgg aaactgtttc atccgcgggt gagcgggagt ccgccgggcg
      421 ggccgccggt ccgatccggg ttcaggtggc gatcgcgccg atcgcgacgc tggtcgagcg
      481 ggtcgggggc gagctggtgg aagtcggggt gctgaccgat gcgggacaga atccggagca
      541 gtttcaaccg acggcgcggc agatcaccga actttcggcg gccgatctgt atctcacgct
      601 cgagctgcca ttcgagcgga atctgaccga gcggctggcg ccaccgccgg cggggccgcg
      661 gatcgtttcg ctcgtggagg ggctggcgct gcggacgatg ccgggccatt tccacgccgg
      721 ggaggacgac gcgacgacgg cgccgcccgg cgcggcggcg catgaatacg cgggcgccgc
      781 cgacccgcac gtctggctcg acccggtgct gaccgcgcag atggcgcagc gggcggccga
      841 ggcgctgagc ctggccgcgc cggatcgggc ggaaacgttc agccagaacg ccgccgccgc
      901 gcgcgccgaa ctggaacgcg tggacgccga actgcgggag ctgctgggtc gcgcgcgggg
      961 ccgggcgttc atgatctacc atccggcgct gggctacctt gccgaccgtt acgggctgcg
     1021 ccagttgccg atcgaggtgc agggcaagga gccggccgcg cgcgagctga gccggacgat
     1081 cgagcgcgcg cgggccgaga gcattcgcac gattttcatc gagcggcagt tcgacaaacg
     1141 caatgcgatg gcgatcgcgc aggcgatcgg ggcggcggtg gaggagatcg atccgctggc
     1201 gccggattac atggagaaca tgcgccggat cgggcaggcg ctggccgcgg cgctgaagcc
     1261 ggtggaatga gtcgcgccgg ctgggaatgg ggctagttcc agcggcgggc ggtcatcgtg
     1321 atgcgtccgg cgtcgtcgac ctgctcctcg acgatgacgg cattgacgcg caggagctgc
     1381 tcggcgaggc ggtggtaggc gaacttgcgc acgatctcgc gggcgaattc gtcggcgcgg
     1441 aggcgcaggt cgagccgcgt cttgttgcgc ggtcccatcg cgtcgatgaa gaatttgccg
     1501 cgggcgtcct tgcggaaaac gagaacgaaa tcctcgcgtt cgaagatcag ccgttcctcc
     1561 ttgccgattt cgtctgaaat aatctcggcc agttcggagt cgagcgggac gcgctcgatg
     1621 cgtctggcgt ccagctcgcg gcgcaggcgg ctctggaagg cgggcgagcg gggatcgctg
     1681 aggagctgat agcccttggc cgccgccacg gcgccgacga tcggcagcag ggcgggccag
     1741 ctccagccca gcgcggcggt taaggcgatg atggcgccca tggcgttgcc tcggggggat
     1801 cgatggggcc gctttcaccc taggagcccg taagagctcc gcgggttgcg cgctatccac
     1861 acggcgccgc gccgcggcgg gagcctagtc cagcgcctcg aggacggcga gggcgtcctc
     1921 ggcggagccg aaggggggca tgatgtaggt cccctggacg tagggcagcg cccggcgcag
     1981 ggcctcgcgg gcgatttccc agccggcgcg ccgctggtcg gcgccctcgg gggcggcctc
     2041 catccgggcc agcacctcgg cggggatcgc catgcccggc acgttgttgt gcaggaagag
     2101 ggcgttgcgc aggctgcgca gcgggaggat gccgatcagg atcggcaagc cgagcggggc
     2161 ggcccgttcg atgaaccgct cgaagacttc ggcgttgtag accggctggg tcatgacgta
     2221 gcgcgcgccg gcgtcgcgct tgcgctgaag gcgctcgatc tcgatgttga tgtcctgcgc
     2281 gccgggattg gcgccgaccc cctggaagaa gcgcgtgggt tcgccgatcg aattgccggc
     2341 caggtcgcgc ccctggttga ggcgggtgac gatgcgggtc aggccgatgg catcgacgtc
     2401 gaagacggcg gtggcgtcgg ggtagtcgcc catcttgggc gggtcgccgg tgatgagcag
     2461 gatgttgtgc aggccgaggg cgttggcggc gagcaggtcg gcctgcatcg ccagcaggtt
     2521 ccggtcgcgg cagcagtagt ggatgatggc ctccatgtcg aaatcgcgct tgaggatgag
     2581 ggccatcgag gaggggttga tgcgggccag ggcgcggggg ccgtcggcga tgttgatgac
     2641 gtcgacgcgg cctgaatcgc ggagtttgcg ggccgactca agggccttgg agatgtcggt
     2701 tccctgcggc gggtcgatct cgacgctgac gacgaactcg ccggcggcga ccttgcgcgc
     2761 gaaccgcgtg gcgaccggcc ggttggcctg ttcgaccggt tcggcttcgc tcgggccgac
     2821 catctgaatg ccggcggatt tctgcgcgcc gggctggagc gcgctcaggg cgttgcgcat
     2881 cgccttgatg tgcgccggcg tggccccgca gcacaggccg aggacgcgca caccctcacg
     2941 gaacatccgc cgggcataga cgccggtgaa ctcggtggtt gccagatgaa tcgtgcggtc
     3001 ctcaatcgtt tcgacgccgc cgatgaacgg ctgggcggcg agcgtcaggc cgggggcggc
     3061 cttggccatc cgccgcatca ggtgaaacat gccgtcgggc ccggcgccgt cgttgaagcc
     3121 gccggcggcc ggggcgagcg cttcgaggcg gcgggcgacc tgttccagat cgaggccgtc
     3181 ggccgtgttg ccctcggcat cgggcgtcag cagcgcgacg atcgggaggt ggggggcggc
     3241 ttcgcgggcg gcggcgacgg ccgcctccag ctcgcgcatt tcgcggaagg tttcgagcac
     3301 gagcagatcg acgccggctt cggcgagcag gccggtgtgt tcgaggaaca ggtcatgcgc
     3361 gcgggcggga tcgatgccgt cgtcgccggg ggcgccgaaa tggcggccgc tggggccgac
     3421 cgagccggcg acccagattt tctggccgcc ggcgtcgcgc gccagacgga cgccggcctg
     3481 gatgatcgcg cgcatttcgt cgggctcgat gccgtggcgc gccagtttga tgcggttggc
     3541 gccgaacgta ttggtctcga gcacctcggc gccggagcgg acgtaggcgg cgtgcagctc
     3601 gcgcaccagg tcgggcttga gcaggttctg ctgctcgaag cactgggtga tgaagacgcc
     3661 gcgctgatag agcatggtgc ccatgccgcc gtcgaacagg acggcgcgct cggccagcgc
     3721 ccgcatgaag gggtggttcc ggaggctggg gggcgggggc gtaaccgaca tgaaggactg
     3781 ggcctgactt cctggaatgg ttggggatag gtggttgttc gactcccttt ttatgctaga
     3841 atccgcaatg gctgtccacc gccttttcca gccttggacg gatgccgcga gagccagcag
     3901 ggtgattccc gcttcatgtt tcgacgcttt gatcgtctca aaccgtatct ctggcggcac
     3961 cgcaaccctc tcctgctcgg catggcctgt ctggtggcct cgaattacct ggatatccgg
     4021 atcgccgtga tgctgggggc ggggatcgat gccgtcgggc tgccagcggg cccgttcggc
     4081 gcggtcaagg cggagctgct gacgatcttt ctgctggtga cgctggtgct ggggatcggc
     4141 gcgggcgtga cgcggttcta catgcggcgg ctgatcatca atacgtcgcg caacgtcgag
     4201 tatgagtttc gcaacgatct gttcgggcat ctgctgcgac tgccggcgtc gttttatgac
     4261 cggttccgca ccggcgacgt gatggcgcgc gcgacgagcg acatcgaggc gatccgcatg
     4321 gtgctgggtc cggcgatcat gtatgtcgcc aacagcctgg tgctgtttcc gatggcggtg
     4381 tggcagatgg cggtgatttc gatgcgcctg atgctgctgt gtttcgtccc gatgctgctg
     4441 gtgatcccgg tggtctatta ttttcggtcg cggatcaaca tccgggcgcg gctggtgcag
     4501 gaagccaaca gcgacctgtc ggccaacgtg caggagaatc tgtcggggat gcgcgtgatc
     4561 aaagtgtacg cgcgcgagca gacgcaggcg gaggcattcg accggctgtc gcagcgctac
     4621 gtgaaggcca acatggatct ggtgcggctg caggcggtct tcttccccgc gctgagcctg
     4681 atgatcggcg gcgtggtggc gatgctgctg ctgggcggcg gcgtgatgat catccgcggg
     4741 acgctgacgc tgggggcgat catcacgttt ttcgcgctgc tgatgacgtg catctggccg
     4801 ctggcggcgt tcggctggat catggcgcag cttgagcggg gagcggcgtc gatgaagcgc
     4861 gtcaacgaga ttttcgaggc ggcgcccgag gcgtcggacg agccggaggc cgcggcggag
     4921 gatctggcgg ccggcgcgcc ggcgatcgcg ccggtgatgc gcggcgagat ccggctgcgc
     4981 gacctgacgt tccgctatcc ggggagcgag cggccggcgc tggccgggat cgatctggag
     5041 atccccgccg gcgggacgct cgggctgacg gggccggtcg gctgcggcaa gtcgacgctg
     5101 gcgcacctgc tgatgcggcg ttacgatccg ccgcgcggcg cgctgttcat cgacggccgc
     5161 gacatcctgg actggccgat cgccgcgctg cgccggcaga tcgggatcgt cgatcaggaa
     5221 ccctttctgt tcagcgacac gatcgccgcc aacgtcgcgt tcggggtggc ggagcaggag
     5281 gcggagcggc tggcgccgcg gatcgagcgg gtgacgcgcg tcgcgcagct cgacgacgaa
     5341 gtgcgcgggt ttccccaggg ctatgagacg attctcggtg agcgcgggat caatctgtcg
     5401 gggggccagc gccagcgttc ggcgctcgcg cgcgccttgg cgcgcgaccc cgcgctgctg
     5461 attctggacg atgcgctggc cgccgtcgac acgcacaccg aggaggcgat tctgcgcggg
     5521 ctgcgcgaac tgctcggcca gcgcacgacg ctgatcattt cgcaccggat ctcgaccgtt
     5581 tcgctcgccg accggatcgt ctacctggaa gcggggcggg tagccgagca ggggacgcac
     5641 gaggaactgc tggcccggcg cgggaaatac tggatgctgg cgcagcgcca gcggctggcc
     5701 gaggagatcg agcggaccgc ctgaggcgcg gcgcgcggca ccatgagcaa actgaagcag
     5761 gccctcgccc ccgatgaagc gctcgcctac cgcaggcgga tcgacacacg gctgctcggg
     5821 cggctgtggg aattcgtgcg cccgtatcag gggatcgtcg gtttcacggt gctgctgtcg
     5881 ctggcggcga acgcgctgcg cgtcctgcag ccgctggtgg ggatgtatat catcgaccac
     5941 cagatcagcg agggcgacgt caatgggctg atcctgatgg tgctgttgtt catcgggctg
     6001 ctgctgctgg cgtcggcctg cgacgtcgcg ttcacctggt tcatctcgtg ggtcgggcag
     6061 cgggcgatgg atacgatgcg ccggcggctg atccgccaca ttttcgcgca ggacacggtc
     6121 ttcttcgatc gcaacccggt gggccggctg atcacgcggc tcacgtcgga cgttgaaacg
     6181 ctcaacgagc tgtttgcctc ggggcttgtc gcggtgatgg gcgagctgct gatcatcatc
     6241 ggcgtgatcg cgctgatgct gacgcaaagc gccaagttga ccggggtggt gctgctcagc
     6301 gcgccgttca tcctggcggt ggcgattttc tttcggcgcc attcaagaaa atggtatctg
     6361 gagacgcgcc gggcactggc cacaatgaac gcctatctgc aggaaaacgt ggcggggatg
     6421 cggacggtgc aggcgttcaa ccggcagcgg cgcaatcacc ggcagttcgc cagcctgaac
     6481 aacgaatatc gggtcgccaa cctgcacacg atttttgcct tcgcgctgtt ctttccggcg
     6541 atgtcgttca tatcgtcgat ggcggtggcg gcggtgatct gggtcggcgg gcgcgatatg
     6601 atcgcgggcc ggggggcggc ggcggggctc agttttggcc agctcttctt cttcgtgcag
     6661 tgcgttcaca tgctgttcgc gccggtccgg tcgttgagcg agaaatacaa cctgttgcag
     6721 gcggcgatgg catcctccga acggatcttc aaactgttcg accagcggcc gacgatcgac
     6781 gcgccgcgcg agccggcgcc gatccggccg ctgaccgacg cgatccgctt cgaggacgtg
     6841 cggttcgcct acgtgccgga tgagccggtg ctcaagggga tcagcttcga gatccggcgc
     6901 gggcagtcgg tggcggtcgt cggcgcgacg ggggccggca agacgacgct gatcaatctg
     6961 ctgacgcggt tctacgacgt aacgagcggg cgcatcacgt atgatggggt cgatctgcgg
     7021 cggttcgatc cgcgggcgct gcgccgccag ttcgccgtcg tcctgcagga ggttttcctg
     7081 ttctccggca cgatcgccga caacctgcgg ctggccaacc cggagctgac cgaggaggag
     7141 atgtgggagg tgctgcgcca ggtgcgcgcg gaggacttcg tcgcctcgct ggcggggggc
     7201 ctgcacgccg aggtttccga acggggggga acgttctcga ccggccagaa gcagcttctc
     7261 gccttcgccc gcgcgctggc ctccgatccg caactgctga tcctcgacga ggcgacggcc
     7321 aacatcgaca cggaaaccga gcagcgcatc caggaggcga tcgggacggt actggcgggg
     7381 cgcaccgcgc ttgtaattgc ccaccggcta tcgacgatcc agcgcgccga ccggattctc
     7441 gtgatgcacc acggccagat ccacgaagcc gggacacacg ccgaactgct ggcgatcgat
     7501 ggcctctatc gccgactcta tgagttacag taccgccatg aagccgtcgc cgatggcggg
     7561 gatccggcgg cgcccgccgc cgccgagcag ctaaccaatg aaagccaggc cctatgaccg
     7621 cctcgcgcgc ctcatatcta atggaacccc gccggattga atttcaggat gaggcgcgtc
     7681 cggcgcccgg ccccggcgag gtgctgatcg aattgaccgc cgtcggcgtc tgcggctccg
     7741 acgtgcactg gtacctggac ggacggatcg gaacgacgcg gctggagggc ccgctgacgc
     7801 tcggccacga gccggcggga cgtgtcgtgg aggtcggcgc cggggccgat ccggcgctca
     7861 agggcaagcg cgtggcgatc gaaccggcga tcgcctgcgg ccattgcccg ttctgccagg
     7921 acggcgacac caacctctgc cctcacgtcc ggttcatggg gacgccgccg acgcaggggg
     7981 ccttccggca gtggacgacc cacccggccc atctggtggc cgagctgccc gcgacggtga
     8041 gcgacgaggt cggggccctg ctcgagccgc tggcgatcgc gatccacgcg accgatctgc
     8101 tcaagccgcg ggccggggcg acgtgggcga tcgtgggggc cgggccgatt ggcctgtgcg
     8161 cgctggaggc ggtcaagctg accggcccgg gctgtgtgat gattgtcgag ccgctggagt
     8221 atcggcgcgc gctggcgcgg tcgatggggg ccgacgtcgt gctggcgccg gagcaggacg
     8281 atctggcggc ggctgtcgcg cgcgcgaccg gcggttacgg cgccgattac gtgattgaag
     8341 ccgccggcaa accggccagt tttgagttga tgacgcaggt ggcgcggccc ggcgcgaaga
     8401 tcgccgtcgt cggcatcgac ccgcacgacc ggttcggctt caacaattcg gtcggacggc
     8461 gcaaggggct gacgctctac atggtgcgcc gttcgcggcg gacgctggag cgcgccatcg
     8521 ccatcaccgc cgccggccgg tggcggcccg acccgatcat cacgcatcgc cggccgtttg
     8581 gcgatctggc ccgttcgctg gagcgggtgg ccgggttcga ggatggggtg atcaagacga
     8641 tcatcgatcc gcgccgctaa gggagggggc gcccgccgta cggccggcgg cggtggcgtg
     8701 gcagcggcct ccaactggac ggcgagagac cgtcaattag cgcttgcatc cgcgcgccgt
     8761 ccgttccaag gttcctctca cgaatcggct tggtgggtga agctagcgtt gaatgccctg
     8821 gcgttgcagc taatccagat gtcgccggcc gacgaaccgc cggaggccgg cgcgccggag
     8881 ccgggcggtc gccccgagcc gccgcggtca acgcggccgg accgcggccg tcagcgcagc
     8941 ggcgcgccgg agacgcccgc ggcactctcc tggcaggcgc ggcccggttg gcgcggacgg
     9001 gcgctggccg cctggatcca attcgatgtg gtcgacttgc cgtggctgca aatggcgtat
     9061 gaagggcgcc gccgcgacgg gctgacctgc gtcaagctga gccggtcgcg ccacgttttg
     9121 cgcgtgcggc gtcccgagcg attcggcgcg cgcgatccgc gcggggtcta cgtcaagcgc
     9181 tacctgatca atacgccgcg gcggcggctg ggcaacctgc tcagcggggg caaggcgcgc
     9241 cgcgagttca ggctgggctg gcggctgatc cagcgcgggc tgcggacggc ccggccgctg
     9301 gcctacgtca tcgccgaccc gatgcggatc ctggaccggc aggcgccgcc ggggttcgcg
     9361 cgcgcggcca gtttcctcgt gaccgaggaa gttttgaacg acgggacgat ctacgagtgg
     9421 gcgtgccggg gcgacgccgc cggatgcgag gggttctacc cggcgctcgc gcgctacatc
     9481 gccgcgatgc accagagggg tttctatcat gatgacctga gcggcaaaaa tatttgcgtc
     9541 gcgtcggggg aggaattgcg ggcgtggctg gcagccgacc gcggggccgc gaccgccgcg
     9601 gccaacggcc tgctgagcaa gttcattctg ttcgatgtgg atcacggccg gctgttccgg
     9661 gagggcaagg tgccgttccg gctgcggatg cggaacgtgt tccagattct gcgttcgctg
     9721 cgccatttcg acatgaagac gcgcgccgcg cgccgccagt ttgtggagga ttatctggag
     9781 gcggccgggc tgtcgacggc gcgccggcgc cgcgccgccg agaaatcgat caaccggttc
     9841 gcccgcgccc ggctcgggca tgagttggtt tgaccggagg ggccggttgt tcgattgcca
     9901 cctgaaggcc gtggcggttg ggaatatcga accgataaat cggttctgtt gtggaacggg
     9961 cgctaccgtg gatcgaatgg ccgccgggcg gccatccaac ccacggcttg gatatcgaac
    10021 cgctgcgcgg ttcctgctgc gccgttggcc gcgaatgata atttttcggt gatgcgtcgg
    10081 gagaaggtag caggcgtttc gagagccgag gacgagggac gagagccgga agccgaattc
    10141 tgggaagggc ctttggaggc gaaaacgagg acgagggacg gacgatccgg catggggccg
    10201 cgatatgcgg ccattgtcgg gaacacccgg ggggtgacgt tggtccttga taggcaaggg
    10261 attcgcttcc cgccaggcgg ggcggggcga aagccggagg cggcgtctcc gaggcgccga
    10321 ttcgaatgac gagcgatggg ccgatccgcc ggccgggtcg tcaaaccagc aggagtcgca
    10381 aaatgcacga ggatctcacc gacggcgggc cggctgaacg cgcgccggaa gccccggccg
    10441 atcatccgcc ggcggttgga ccggccctcg gcccggagcg ccgccgccgg ctcaggccgg
    10501 gcaaggcggc
//
LOCUS       DAOPCO010000136        10474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6176_length_10474_cov_4.990018, whole genome shotgun sequence.
ACCESSION   DAOPCO010000136 DAOPCO010000000
VERSION     DAOPCO010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10474
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6176_length_10474_cov_4.990018"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1575)
                     /locus_tag="PLS90_10330"
     CDS             complement(<1..1575)
                     /locus_tag="PLS90_10330"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPK02839.1"
                     /translation="MSERKRRVLIWASSPMNIVVLRPILDVLRHDRRISLFLTGKYHG
                     EDNPLAIARETGLTGLHMVSLDQAKKLPADLFLTADRKRLGHGCKRKVLMFHGVSFKG
                     RSITPKVKWFDRILLVGPYQRRKFAETGALREDDPRFELIGMPKLDRLARGEFDRDQI
                     RAELGVPDGKPMVLYAPTWTEQSSLYHMGEPMLEELARMPEVHVVVKLHDHLYDMSRN
                     SIDWAARLTSKPWDNVRLTRLGDIVPLMAAADILISDASSAANEFTLLDRPILFADAP
                     ELWRGERYGSTADLDTWGRKGGIVIERPADVRAAIRRSLENPAEFSAIRRQIAADLFA
                     YPGESTGRAIAAIYREMGLEPPSAAELELARSPRLAGPPLNILHVASHGDITRGGAVQ
                     MLTMVRQLIRRGHRVSVVLNNAPENVGRFEPRLRELGAARVACFPMQADGVLKRLRIQ
                     RQFRKWYREGNYDVVHTHRQEALGFVLRALRGERVPALLTNRGTTYRFASAADRKAYH
                     SPRLDRIIAVAHAVEAV"
     gene            complement(1572..4091)
                     /locus_tag="PLS90_10335"
     CDS             complement(1572..4091)
                     /locus_tag="PLS90_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02840.1"
                     /translation="MISKPFYTTPPPLVLRVVGSMEPGSVQRNILETLIDLDTRRFRA
                     QVLCVRRRAEWGHALAGHGIPTWSYRRRVAYSRLFRGAVWRMIARLTVAVRKRRPAVM
                     HIHQARLSFPMVMVAKLARVPCIVLHHHADYRAGYWGTLTGFQQTADRWAVRHADVVV
                     ASSHSVAEATSQHYGLPRERIEVVPNGARAPVRHPGARVRPDSVLPVVPEGAAVVGIV
                     GRMRPERGWEEALNAAAELVRRHDHLRFWLVGETRLEEDYPRCLKEQIDRLGLTAFCE
                     VMTNRHDLDVIYGRLTAALFCSRVEGCPVALLDLLASGVPIVAADTPHIRELVGHQRQ
                     ALLTAPENFHEQARALDHLLRDEALRSRLIREGREVAARYSWEQAERAYERIYQRVLE
                     QKRRWVEPVRPRPPDHPVLPALEGPRPLAPVLYLIKTGLDEARAHIGQSLHTITALAR
                     AGQPVTILAPMATSRLEKIVQDAGVTDYDRLCANLTLIRLKEEPRHHVSSTRELERTL
                     RRQFKRGHRILYFRQPRIASLVLPLAHRIGYRVIMENHCPHALWSIHERREFWHAAPT
                     SLHWFRRAVRNDLTYEQDLYQLLDGVINTTRAIDRRVRRLAPQTPTLLLPNGAPNIPD
                     ELPPYGPDYRDIDILYVGKTAIEKGTDQIIRAMEHLPGFLLQIVGGPTEEDLAPFRAL
                     AQALGVEERIVFRSWMPQRDVLTLMRQARVAVHPLAGHGSREWRLFTCPLKILEYMAA
                     GTPVVSTRLPAILELLTDGQDALLAPPADARALAECLRRVLESPQLAESLRLRAFDRV
                     RQFSTEARAAAIVRFLNEISTGPAPVRTPEAAPADAESLSA"
     gene            complement(4116..6104)
                     /locus_tag="PLS90_10340"
     CDS             complement(4116..6104)
                     /locus_tag="PLS90_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02841.1"
                     /translation="MSLLPRLLPRLWAFKFRLLNIAILSVVAGFLGAFSLIAFKPIIE
                     VMFYQPGEAIEGPQVSPLAPPEDAGSPVIPLPSKIAEKVQFYKNLVQNYVAGHQEAAK
                     QWMLHNKVHALILIGIILLVAQALGAYIKYVVEVMTAKIGLGIQKDLKDELFEHIVRQ
                     DMPFFSRTSTGQIMARLQNDTRKVQRMLMDFTGSTVIDFFNVIGLLCVLLYLSPRLTI
                     YAAVTLPILGGLFYFTGRAIRKLSDKAEEHDAGVFHVSQEALSGIAVVKAFGADGHEV
                     KRFKQATAQTNRRLLTLQRVQGFVGPANDFLSTLAVVLTLVMGGILFLGDRNYLGPDF
                     QTADFTIYLLAITRFYRPIKGLLKLNVTYHRGMASLARIYRIMDIQPTILDAPDAVDL
                     PDRTPRVEFHGVTFAYPRRRNPQPVLENFNLEVEPGEVVALVGPSGAGKTTLINLICR
                     FYDVQEGVVRIAGHDVRHLRQQSLRDRIAIVPQETFLFNDTIHENIAYGRTDATRAEV
                     EQAARLAHAHHFIAALPDGYDTVVGERGGKLSTGQKQRLAIARALLKNPTILIFDEAT
                     ASLDAETERQIRDSMRLICRGRTTFIIAHRFSTVMMADRIVVLENGRIVEQGSHETLY
                     EAGGLYARLCNIQGIFVDTPPEATLSGHPSRSLANGLG"
     gene            complement(6363..>10474)
                     /locus_tag="PLS90_10345"
     CDS             complement(6363..>10474)
                     /locus_tag="PLS90_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK02842.1"
                     /translation="AQVFATKIFNNHGGSILDELINGLPARSPDSLTKVAYDNGARIS
                     SNSWGISMPLFGGQYDQITQIYDRLTRDCNALASGQQPMIFFFAAGNFGPLARSIASP
                     AAGKNIISVGASENCDQGEFDRSGIGPSLSDNLQDIAVFSSRGPTADDRIAPTIVAPG
                     THITGAASDDPNFDGHGVSGRLQVEFGEPPENVKYYPAFQTDYTWSSGTSHSTPLAAG
                     AAVLLYEYFLRTFGIEPSPAMVKAALIATASDMSAGGLNDGSGLPVGPSPNPDAGWGR
                     LNIDRLVHPSTMQFAYDQQTILTSPGQGYDTIIQVTDGNQPLRIVLAWTDPPASLLGQ
                     GSKLVNDLDLRVVDPLNRSFLGNVFEGGVSVEGGIRDTINNVEVIHIPRPVTGIYRVV
                     VQAATLKGDALPNQGGPLEQDFALFVTNGANQTSRGRIAMLDELYGCDDTVEVRLSDS
                     DLIGEGTAEATISSQLTQDEETVVLFEVNAPSGVLDGAIQTDGALSPKPGDGVLTVRH
                     GDTITVRYSEQSAEGEPFVSEDTAVVDCEGALLISQEVFDVTATTARVRVTLNEPANV
                     TIGYGLSCGELNRLSVSPTFGTVHELNLEGLPPCSPVFFNIRATDEAGNSRTFDHNGQ
                     CYAFVTDNQQSVTLFNDNVPSEFTPHWTHGRNFGFDDWDIFENDFAVSNGLSWGSIAR
                     ATINDSFLTMPPLFILPETTLTFWHTFRLESLGPGAAADGAMIEISLDGGTIWGDLGT
                     YIQGAQGYNSTIVAGTGNPLVDRRCWSNGTLGPMEQVTVDLSDFAGETALIRFRLATD
                     SGNTLEAGDVAAWYIDQVTLVSTEPAECVGTLAVLEFDARFISCNKPALRFTITDADL
                     SLANGGIPTTFRLGSSVHPDTVQVFPDNLALIDPKGIWEGFLSLEPGILPNTLRVAGL
                     IDVPEGARVRLSYRDSNVSGSPYVVEDEAPIDCTAPALFSAEPVLIEPNTAVLRIISN
                     EENRVVIHFGTSCDNLNRTETRELIATEIYIGLHDLLPCTPYYYKVELIDRAGESEIF
                     DNFGNCFGFVTSESGSLRDTLEPNPQFQWQTQALAGTNDWTVTFYGQAHSPIHTWFST
                     NASTTKDVVLVTPPVDITPETMLRFYHTYALEAGFDGAVIEISTDGGATWEDLGNQVT
                     QGFYNGVISTQFGSRIAGRRAWTGGQLGPMQPVMVALARYAGEDRRVRFRLVCDTSQA
                     VKGGGWHIDDVEFAFERACPLQFPAPPVLVSPPDDLRNVSFDDSIVLDWNSAAFAESY
                     IVRWGTTFETLNTLVDTTNTETWFPFTGITSGQTIYWRILARNALGVTSSAVRRFTME
                     VIDPNLVAKEMVGMDSGASENVKQAMDINRDGKISVEDVIQGVNLRSIR"
BASE COUNT         1766 a         3461 c         3460 g         1787 t
ORIGIN      
        1 caccgcctcc accgcatggg ccaccgcgat gatccgatcg agccgtgggg aatgatacgc
       61 cttgcgatcc gccgcgctcg cgaaccggta ggtcgttccc cggttcgtca acagcgccgg
      121 cacacgctcg cccctgagcg cgcgcagcac gaatcccagc gcctcctgcc ggtgcgtgtg
      181 gacgacgtcg tagttcccct cgcgatacca tttgcggaac tgccgttgga tccgcaggcg
      241 cttgagcacg ccgtcggcct gcatcggaaa gcacgccacg cgggcggcgc ccagttcgcg
      301 cagccggggc tcgaaccggc cgacgttttc cggcgcgttg ttcaagacca cggagacccg
      361 atgcccgcgc cggatcagtt ggcgcaccat ggtcagcatc tgcaccgcgc ccccccgggt
      421 gatgtcgccg tgcgaggcga cgtgcaggat gttcagcggc gggccggcca ggcgcggcga
      481 tcgggcgagc tcgagttccg ccgccgaggg cggttccaat cccatctcgc ggtagatcgc
      541 cgcaatcgcc cgcccggtcg attcgcccgg ataggcgaac aggtcagccg cgatctggcg
      601 gcggatcgcg gagaattcgg cggggttttc caacgagcgt cggatcgcag cgcgcacatc
      661 cgccggccgc tcgatgacga tccccccctt gcggccccac gtgtcgagat cggccgtcga
      721 accgtagcgt tcgccgcgcc agagctccgg cgcgtcggcg aacagaatcg gccggtcgag
      781 cagcgtgaac tcattggcgg ccgaactggc gtcgctgatc aggatgtcgg ccgcggccat
      841 caggggaacg atgtcgccca ggcgggtcag ccggacgttg tcccacggct tgcttgttag
      901 ccgcgcggcc cagtcgatcg agttgcggct catgtcgtag aggtgatcgt gcagcttgac
      961 caccacatgc acctcgggca tgcgggccag ttcctccagc atcggctcgc ccatgtgata
     1021 gagcgagctt tgctcggtcc aggtgggggc gtagagcacc atcggcttgc cgtccggcac
     1081 gcccagctcc gcgcggatct ggtcgcgatc gaattcgccg cgcgcgaggc ggtccagctt
     1141 gggcatgccg atcagctcaa agcgggggtc gtcctcgcgc aacgctcccg tctccgcgaa
     1201 tttgcggcgc tgatacgggc cgaccagcag gatccggtcg aaccacttca ccttgggggt
     1261 gatcgaacgg cccttgaatg aaaccccgtg gaacatcagc accttgcgct tgcagccgtg
     1321 acccagccgc ttgcggtcgg ccgtcaggaa cagatcggcc ggcagcttct tggcctgatc
     1381 gagcgagacc atgtgcagac cggtcaggcc ggtctcccgc gcgatcgcca gcggattatc
     1441 ctcgccgtgg tatttgccgg tcaggaacag actgatccgc cggtcgtggc gcaggacgtc
     1501 caggatcggc cgcagcacga cgatgttcat cggcgaactg gcccagataa gaacccgtcg
     1561 cttgcgctcg ctcatgcgct cagactttcg gcgtcggcgg gtgcggcctc gggcgtccgg
     1621 acgggcgccg gtccggttga gatttcgtta aggaaccgga cgatggccgc cgcgcgcgcc
     1681 tcggtgctga attggcgaac gcggtcgaac gcccttaggc gcaggctttc ggccagctgc
     1741 ggactctcca ggacccggcg caggcactcc gcgagggccc gcgcgtcggc gggcggggcg
     1801 agcagcgcgt cctggccgtc ggtcagcaat tccagaatcg ccggcagccg ggtcgagacc
     1861 accggcgttc ccgccgccat gtattccagg atctttagcg ggcaggtgaa cagacgccac
     1921 tcacgcgaac cgtgcccggc cagcggatgg acggcgacgc gcgcctgccg catcagtgtc
     1981 aagacatcac gctggggcat ccatgagcgg aatacgatgc gctcctccac gccgagcgcc
     2041 tgggcgagcg cacgaaacgg cgccagatcc tcctcggtcg ggccgccgac gatttgcagc
     2101 aggaagcccg gcaggtgttc catcgcgcgg atgatctggt cggttccctt ctcgattgcc
     2161 gtcttgccga cgtacaggat gtcgatgtcg cggtagtccg gcccgtaggg gggcagctca
     2221 tccggtatgt tgggcgcgcc gttgggcagc agcagcgtcg gcgtctgggg ggccaggcgg
     2281 cggacgcggc ggtcaatcgc ccgcgtcgtg ttgatgacgc catcgagcag ttgatacaga
     2341 tcctgctcgt aggtcaggtc gttgcgcacg gcccggcgga accagtgcag cgacgtcggc
     2401 gccgcatgcc agaattcgcg ccgttcgtgg atcgaccaca gcgcgtgcgg acaatgattc
     2461 tccatgatga cgcgatagcc gatgcggtgg gccagcggca ggaccagcga ggcgatgcgc
     2521 ggctgccgga aatagagaat ccgatgcccc cgcttgaact gccggcgcag cgttcgttcc
     2581 agctcgcggg tgctcgaaac gtgatggcgc ggctcttcct tcaaccggat gagcgtcaga
     2641 ttcgcgcaca ggcggtcgta atcggtcacg ccggcgtcct gcacgatctt ctccaaccgg
     2701 ctggtcgcca tcggcgccag gatcgtcacc ggctggccgg cgcgagccag ggccgtgatt
     2761 gtgtggaggc tctgaccgat gtgcgcgcgg gcttcatcca ggccggtctt gatcaggtag
     2821 agcaccgggg caagcggacg tggtccctcc agggccggca ggaccgggtg atcgggcggc
     2881 cgcgggcgaa ccggctccac ccagcggcgc ttctgctcca agacgcgctg gtagatccgt
     2941 tcatacgcac gctcggcctg ttcccaggaa tagcgggcgg cgacctcgcg cccttcccgg
     3001 atcagtcggc tgcgcaacgc ttcgtcgcgc agcagatggt cgagcgcgcg cgcctgctca
     3061 tggaaattct ccggcgccgt cagcaaggcc tggcgttgat gtcccaccag ttcgcggata
     3121 tgcggcgtat cggcggcgac gatcggcacg ccgctcgcca gcaggtcgag cagcgccacg
     3181 gggcacccct cgacccgcga gcagaacagc gccgcggtca accggccgta gattacatcg
     3241 agatcgtggc ggttggtcat gacttcgcag aacgccgtca ggcccaaccg gtcgatctgc
     3301 tccttcaggc aacggggata atcctcctcc aggcgggttt cgcccaccag ccagaagcgc
     3361 agatggtcgt ggcggcggac cagttcggcc gccgcgttga gcgcctcttc ccagccccgt
     3421 tctggccgca tccggccgac gatgccgacc acggcggcgc cctcgggtac gaccggcaga
     3481 acgctgtcgg ggcgcactcg cgcccccgga tggcgcaccg gcgcgcgcgc gccgttgggc
     3541 accacctcga tccgctcgcg cggcaggcca tagtgctgac tggttgcctc ggcgaccgaa
     3601 tgcgagctgg cgacgacgac gtcggcgtga cgcaccgccc accgatccgc cgtctgctga
     3661 aagcccgtca gcgtccccca atagccggcg cggtagtccg catgatgatg cagcacgatg
     3721 catgggacgc gcgccagctt ggcgaccatc accatcggga agctcagccg ggcctgatga
     3781 atgtgcatga cggcgggtcg ccgcttgcgc accgccacgg tcaggcgcgc gatcatccgc
     3841 cacaccgccc cgcggaacag gcgcgagtag gcgacgcggc ggcggtagct ccaagtggga
     3901 atcccgtggc ccgcgagcgc gtgtccccac tccgcccgtc gacggacgca gagcacttgg
     3961 gcccgaaacc gccgcgtgtc cagatcgatc agcgtctcca ggatgttgcg ctgcaccgag
     4021 cccggttcca tcgagccgac gacgcgcagc accaggggtg gaggggtggt gtagaagggc
     4081 ttggagatca tcggcgccgg tccccgcgag tgtactcaac cgagaccgtt cgcgagcgac
     4141 cgcgacggat ggcccgacag cgtcgcctcg ggcggcgtat ccacgaaaat cccctgaatg
     4201 ttgcacaacc gggcgtacag cccgcccgcc tcgtagagcg tttcgtgcga gccctgttcg
     4261 acgatccggc cgttctccag cacgacgatc cggtcggcca tcatcaccgt ggagaagcgg
     4321 tgggcgatga tgaacgtggt gcgcccgcgg cagatcaggc gcatgctgtc gcggatctgc
     4381 cgttccgttt cggcgtcgag cgacgccgtt gcttcgtcga aaatcagaat cgtcgggttt
     4441 ttcaagagcg cgcgggcgat cgccagccgc tgcttctggc ccgtggagag cttgccgccg
     4501 cgctcgccga cgaccgtatc gtatccgtcg ggcagggcgg cgatgaaatg atgggcgtgc
     4561 gccagccgcg cggcctgctc cacttccgcg cgcgtcgcgt cggtacggcc ataggcgatg
     4621 ttctcgtgga tcgtgtcgtt gaacagaaag gtttcctgcg ggacgatcgc gatccgatcg
     4681 cgcagcgact gctggcgcag gtggcgcacg tcgtggcccg cgatccgcac caccccttcc
     4741 tgcacgtcgt agaaccggca gatcaaattg atcagggtcg tcttgcccgc gcccgaagga
     4801 ccgacgagcg ccaccacctc gcccggttcg acctccagat tgaagttctc gagcaccggt
     4861 tgcgggttgc gtcgccgcgg gtaggcgaac gtcacaccgt ggaactccac gcgcggcgtc
     4921 cgatcgggca ggtcgacggc gtcgggcgcg tccaggattg tcggctgaat gtccatgatc
     4981 cgatagatcc gcgccagcga ggccatgccg cgatggtagg tgacgttcag cttgagcagc
     5041 cccttgatgg gccggtaaaa ccgcgtgatc gccaagagat agatcgtgaa atccgccgtc
     5101 tgaaagtccg gccccaggta attgcggtcg ccgaggaaca ggatgccgcc catcaccagc
     5161 gtcagcacaa cggccagcgt gctgaggaag tcattggccg gcccgacaaa gccctgaacc
     5221 cgctgcaagg tcaacaggcg gcggttggtc tgcgccgtcg cctgcttgaa gcgtttgacc
     5281 tcatgcccgt cggcgccgaa cgccttgacc accgcgatgc ccgagagcgc ctcctgcgag
     5341 acatggaaaa cgccggcatc gtgttcctcc gctttgtccg acagcttccg gatcgcccgg
     5401 ccggtaaaat agaacaggcc gccgaggatc ggcagcgtca ccgccgcgta aatcgtcagg
     5461 cgaggggaga gatagagcag cacgcagagc aggccgatga cgttgaagaa atcgatcacc
     5521 gtggagccgg tgaagtccat cagcatccgc tgcaccttgc gcgtgtcgtt ctgcaggcgc
     5581 gccatgatct ggccggtcga cgtgcgcgaa aagaacggca tgtcctgccg cactatgtgc
     5641 tcgaacaact cgtccttcag atccttctgg atccccaggc cgatcttcgc cgtcatcact
     5701 tccacgacat acttgatgta ggcccccagc gcctgcgcca ccagcaggat gatcccgatc
     5761 agaatcagcg catgcacctt gttgtgcagc atccattgct tggccgcttc ctgatgaccg
     5821 gccacgtagt tctgcaccag gttcttgtag aactgcacct tctcggcgat cttgctcggc
     5881 aggggaatca ccggcgatcc ggcatcctcc ggcggggcaa gcggcgagac ctggggccct
     5941 tcgatcgctt cgcccggttg atagaacatc acttcgatga tcggcttgaa cgcgatcagc
     6001 gaaaatgcgc ccagaaaacc ggccaccacc gacaggatcg caatgttgag cagacggaac
     6061 ttgaaggccc acaggcgcgg gagaagacgg ggaagcaagg acatgccgat gcaagaacct
     6121 cgtccagcgg ggtcaagtgg tcggaccgat acgtcctgcc gcatcggcct aaccttggaa
     6181 ttaaagcacc ttgctcgctg aaccgtcaag ggaaccgcca tcgcccgggc gggcgccggg
     6241 cgatgcgtgg atcggcggcg gtggccggcg cggcatcggt cgccctggac cagaataacc
     6301 aaggcggggg cggcgggctg gtcggcccac gcgcccccgc gatcaagcaa cgccacgccg
     6361 gcctagcgga tgctccgcag gttcaccccc tggatcacat cctcgacgct gatcttgccg
     6421 tcgcggttga tgtccatcgc ctgcttcacg ttttccgagg cgccgctatc catcccgacc
     6481 atttccttgg ccaccaggtt gggatcgatc acctccatcg tgaagcgccg caccgcactc
     6541 gaggtcacac ccagcgcgtt gcgggccaga atccgccagt agatcgtctg gcccgacgtg
     6601 atcccggtga acgggaacca ggtctcggtg tttgtcgtat cgacgagcgt gttgagcgtt
     6661 tcgaaggtcg tgccccagcg gacgatgtag gattccgcga aggcggcgct gttccagtcc
     6721 agcacgatcg aatcgtcgaa cgagacgttg cgtagatcgt cgggcggcga caccagcacc
     6781 ggcggggccg ggaattgtag cgggcaggcc cgttcaaagg cgaattcgac gtcgtcgata
     6841 tgccatccgc cccccttgac cgcctgcgag gtgtcgcaga ccaggcggaa ccggacgcgc
     6901 cgatcctcac ccgcgtagcg ggccaacgcg accataaccg gctgcatcgg gcccagttgg
     6961 ccgcccgtcc aggcccggcg cccggcgatc cggctgccga actgcgtcga aatcacgcca
     7021 ttgtagaacc cttgcgtgac ctggttgccc aggtcctccc acgtcgcccc gccatcggtg
     7081 gagatctcga tcacggcgcc gtcgaacccc gcctccagcg cgtaggtgtg gtagaaccgc
     7141 agcatcgttt ccggcgtgat gtcgaccggc ggcgtcacca gcaccacgtc cttcgtcgtg
     7201 ctggcgttgg tcgagaacca cgtgtggatc ggcgaatggg cctggccgta gaacgtgacc
     7261 gtccagtcgt tggttcccgc cagcgcctgc gtctgccatt ggaactgcgg attgggttcg
     7321 agtgtatcgc gcaacgagcc cgattcactg gtgacgaacc cgaagcaatt gccgaaattg
     7381 tcgaagatct cgctttcgcc cgcccggtcg atcagctcca ccttgtagta atagggggtg
     7441 caggggagca ggtcgtgcag gccgatgtaa atctccgtgg cgatcagttc gcgggtctcc
     7501 gtccggttca gattgtcgca gcttgtcccg aagtggatga cgacccgatt ctcctcgttg
     7561 gagatgatcc gcagcaccgc cgtgttcggt tcgatcagta ccggctcggc gctgaacagg
     7621 gcgggcgccg tgcagtcgat cggcgcctca tcctccacga cgtaggggga gccgctgacg
     7681 ttgctgtcgc gataggacag gcggacgcgc gccccttccg gcacgtcgat cagccccgcc
     7741 acccgcagcg tgttgggcag gatgcccggt tccagcgaca ggaagccttc ccagatcccc
     7801 ttgggatcga tcagcgccag attgtcgggg aagacctgca ccgtgtcggg atgaacggac
     7861 gaaccgagcc ggaatgtcgt cgggatgccg ccgttggcca gcgacagatc ggcgtcggtg
     7921 atcgtgaagc gcagcgcggg cttgttgcag gagatgaagc gcgcgtcgaa ctcgagcacc
     7981 gccagcgtgc cgacgcactc ggccggctcg gtcgacacca gcgtcacctg gtcgatatac
     8041 caggcggcca cgtcgcccgc ttccagcgtg ttgccgctgt cggtggccag gcggaaccgg
     8101 atcagcgccg tttcaccggc aaaatccgac agatcaaccg tcacctgctc catcgggccg
     8161 agcgtgccgt tgctccagca acggcggtcg acgagcggat tgccggtccc ggcgacaatc
     8221 gtcgaattgt agccctgcgc cccctggata taggtgccca ggtcgcccca gatggttccg
     8281 ccgtcgagcg agatctcgat catcgcgccg tcggccgccg cgcccgggcc cagcgactcc
     8341 agccggaacg tgtgccagaa ggtcagcgtc gtctcgggca gaatgaacag gggcggcatc
     8401 gtcaggaatg aatcgttgat cgtcgcgcgc gcgatcgagc cccacgacag accgttgctg
     8461 accgcgaaat cgttctcaaa tatatcccag tcgtcgaagc cgaagttcct gccgtgcgtc
     8521 caatgcggcg tgaactcgct cgggacgttg tcattgaaca gcgtgacgct ttgctgattg
     8581 tcggtcacga acgcatagca ttggccgttg tggtcgaacg tgcggctgtt gcccgcctcg
     8641 tcggtggccc ggatgttgaa gaacaccggc gagcaggggg gcaggccctc caggttcagc
     8701 tcgtggaccg tcccgaaggt gggcgaaacg gaaagccgat tcagttcgcc gcagctcaac
     8761 ccatagccaa tcgtgacgtt cgccggttcg ttgagcgtca cgcgcacccg cgccgtggtg
     8821 gcggtcacgt caaagacttc ctggctgatc agcagtgccc cttcgcagtc gacgaccgcc
     8881 gtatcttcgc tgacgaacgg ttcgccctcg gcgctctgct cgctgtagcg gacggtgatc
     8941 gtgtcgccgt ggcgcaccgt cagcacgcca tcgcccggct tgggcgacaa cgcgccatcg
     9001 gtctggatcg cgccgtccag cacgcccgag ggcgcgttca cctcgaacaa caccaccgtc
     9061 tcctcgtcct gggtcaactg gctggagatc gtcgcctccg ccgtcccctc gccgatcaga
     9121 tccgaatcgc tcaggcgaac ttcgacggtg tcgtcgcagc cgtagagttc gtcgagcatg
     9181 gcgatgcgcc cgcgcgaggt ctgattggcc ccgttggtga caaacagcgc gaaatcctgc
     9241 tccagcggtc cgccctggtt cggcagcgcg tcgcccttca gcgtggccgc ctgcaccacg
     9301 acgcgataga tgcccgtcac cggccggggg atgtggatca cttcgacatt gttgatcgtg
     9361 tcgcggatgc cgccttccac ggagacgccg ccctcgaaga cgttgcccag gaagctgcgg
     9421 ttgagcgggt ccaccacgcg cagatcgagg tcgttgacca gcttgctgcc ctggcccagc
     9481 agcgaagcgg gaggatcggt ccaggccagc acgatccgca gcggctggtt cccgtcggtc
     9541 acctgaatga tggtgtcgta gccctggccg ggcgaggtca gaatcgtctg ctgatcatag
     9601 gcgaactgca tcgtgctcgg gtggaccagg cggtcgatat tgagccggcc ccagcccgcg
     9661 tcggggttgg gcgacggtcc gaccggcagg ccgctgccgt cgttgagtcc gccggcgctc
     9721 atatcgctcg ccgtcgcgat cagcgccgcc ttcaccatgg cggggctcgg ttcaatgccg
     9781 aacgtccgca ggaagtattc gtagaggaga acggcggcgc cggccgccag tggcgtcgag
     9841 tggctcgtcc cactgctcca ggtgtaatcg gtctggaagg ccggatagta tttgacgttt
     9901 tcgggcggct cgccgaattc gacctggagc cggccgctga cgccatggcc gtcgaagttc
     9961 gggtcatcgg aggccgcgcc ggtgatgtgg gtgccgggcg cgacgatcgt cggcgcgatc
    10021 cggtcgtcgg ccgtcggacc gcgcgaggag aacacggcaa tgtcctggag attgtcggag
    10081 agcgacgggc cgatccccga ccggtcgaac tccccttgat cgcagttttc cgaggcgccg
    10141 acgctgatga tgttcttgcc ggcggcgggc gaggcgatcg agcgggcgag cgggccgaaa
    10201 ttgcccgccg caaagaaaaa gatcatcggc tgctggccgc tcgccagcgc gttgcagtcg
    10261 cgcgtcagcc gatcgtaaat ctgggtgatc tggtcatact ggcccccaaa cagcggcatg
    10321 ctgatgcccc aactgttgct cgaaatgcgc gcgccgttgt cgtaggccac cttcgtcagg
    10381 ctatcgggcg accgcgctgg cagaccgttg atcaactcgt cgaggatcga gccgccgtgg
    10441 ttgttgaaaa tcttggtggc gaacacctgg gccc
//
LOCUS       DAOPCO010000137        10402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6225_length_10402_cov_4.477046, whole genome shotgun sequence.
ACCESSION   DAOPCO010000137 DAOPCO010000000
VERSION     DAOPCO010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6225_length_10402_cov_4.477046"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLS90_10350"
     CDS             complement(<1..727)
                     /locus_tag="PLS90_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020036461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HPK02843.1"
                     /translation="MKAAYIVQTGEPDVIQYGDLPTPEPEPHQVLVRVRAVSVNPIDT
                     YIRSGMIPFELPKPYIIGCDFAGTVEATGSEVTRYKPGDRVWGSNQGLLGRQGTFAQY
                     VAVNEAWVYRTPTGVDDEEVAALALVGITAHLGLFEKARLKRNETIFVAGGSGGVGSV
                     VIQMAKLAGARVITSAGNEAKVEICRELGADEVIRYRERDLAEAVRQLAPEGVHVWFD
                     VRREPDLMQAVPLLRPSGRMVIMA"
     gene            complement(772..1278)
                     /gene="tpx"
                     /locus_tag="PLS90_10355"
     CDS             complement(772..1278)
                     /gene="tpx"
                     /locus_tag="PLS90_10355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPK02844.1"
                     /translation="MERPGAITFKGAPMTLVGQREVKVGDPAPDFTVTTGELKDLTLA
                     DLKGQPVVISVVPSLDTGVCQIQTQKFNEQAREHGLRVVTVSCDLPFAQKRFCESFKA
                     NNITCVSDYKSRSFGEAFGVVIKELALLARSVFVLDAEGKVVYREIVSEVTHEPDYGK
                     ALEAVRGL"
     gene            complement(1456..3000)
                     /locus_tag="PLS90_10360"
     CDS             complement(1456..3000)
                     /locus_tag="PLS90_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HPK02845.1"
                     /translation="MAKARKVAVFWPGDYRPMPNKQALPNIEKATAQMEAALRKLGRQ
                     PYRIKGYMTRPSDAIEKLGPIDDPIVGIFVHWVYGPHTVDGVSGKENPLLLASNFSGQ
                     WPGLVGLLNTGACLEAVNRPFSRIWTDASNWTRDRVFMDRLDEWCSTGRIAYSEDELH
                     YAAPVGAAAADNAAAVAAGIRDRRVLAMMLGDTSMGMTNGYFGVRELHKIGFSEHKVD
                     QAWIVDRVQRISPRRVDDALKFCLDRGVKFHWGETNWGEDSSDFTEDATRTQLKMYLA
                     VLDLLDEFKCDCLGWQYQLGLIPLLPPSDFCEGLLNSNARPEGDGQPIMCATEADQGS
                     LVPMELMKRLLARKGLNQTVNFHDVRWGGKVGNRFIWVLLNSGSCSAYAFNHDEKSLK
                     GVHSYRQPKGYFPIPGGTFTGESLPGKMTWARAYLKNNALWMDIGRGEVLKVPAKQRD
                     LFWEGTTPQWPLMLADVGCSMETVMAHYMSNHICVAYGDIFGELVALCQMLGIKVRIL
                     SSKRPE"
     gene            3531..4532
                     /locus_tag="PLS90_10365"
     CDS             3531..4532
                     /locus_tag="PLS90_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK02846.1"
                     /translation="MSGFNENRYDQMHYRRCGRTGLKLPAVSLGLWHNFGAKAEAGNI
                     RAMLRTAFDLGITHFDLANNYGPPPGAAEENFGRVLHDDFAHHRDELIISTKAGYRMW
                     PGPYGDFGSRKYLIASLDQSLKRMKLDYVDIFYHHRPDPETPLEETLEALDIIVRQGK
                     ALYVGVSSYPGALLVRAAETMRQHNWTPIAIHQPHYNMFNRWIETDLLDQTEEYGIGV
                     IPFCPLAQGVLTNRYLDPKNPIPSDSRAGNPDNPFMTAESVERVVGKVRKLNVIAERR
                     GQSMAQMAIAWVLRDPRVTSALIGASRPSQITDCVAALNQLGFTQDELTEIDQILAN"
     gene            4574..5719
                     /locus_tag="PLS90_10370"
     CDS             4574..5719
                     /locus_tag="PLS90_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK02847.1"
                     /translation="MLHDLARLRDGVSRRVSSYDTRGGNADSHFIRDGETFVLADLKG
                     AGIIRHIWFTILAGGDFPRQIVLRMYWDDMDYPSVEAPIGDFFGIGHAQLASLNTVPF
                     SVSATDYLGPKAAFNCYWPMPFATRARITVHNETGADIPAFYSYIDYDQLPSIPDDLG
                     RFHAWWNRNNPCKPPRHPGKDPQFNLTDRDNYLIADIRGRGHFVGCNLSVHNLYGGWW
                     GEGDDMFMVDGQKWPPDLHGTGSEDYFGHAWGMQPENAFIYNGVSYYKKGTEPGAFNE
                     RITVYRYHINDPIIFQKSLRASIEHGHANDRCDDYCSTAYWYQTLPHKKFPPFPKMAD
                     RLPRPDVQLTMQPLDAPIMPTDLRHRSGQAFPPMRVIPPPPPRKKTR"
     gene            complement(6078..7463)
                     /locus_tag="PLS90_10375"
     CDS             complement(6078..7463)
                     /locus_tag="PLS90_10375"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HPK02848.1"
                     /translation="MEYRIEHDSLGEVKVPADALYAAQTQRAVENFPISGTVFPRVFI
                     RALGLVKQCCARVNRELKLLEPAVAELIDRAAQEVIDGKLDGHFPIDVFQTGSGTSTN
                     MNANEVIATRAMQLGDGKTKIHPNDHVNMGQSSNDVIPTAIHVAAYIETSEVLLPGLK
                     RLRETLERRARELDDVVTTGRTHLMDAMPTRLGQEIGGWAYQVRQAIERVEGTLPRLA
                     QLAQGGTATGTGINAHPEFGARVAAALAQATGLPFVESPDHFAAQSAMDSATELSGQL
                     KTSASALMKIANDLRLMNSGPIAGLGEIALPSLQPGSSIMPGKVNPVICEAMMMACAQ
                     VTGNDTAIAICNQMGNFQLNVMLPALAKNLLESITVLGSAARVLADKAVAGFTVNRER
                     IAGLLDKNPILVTALNPVIGYELAAKIAKRAYAEGRSLKEVALEMTDLKAEELDRLLD
                     PAPMTRGGIRK"
     gene            complement(7632..9110)
                     /locus_tag="PLS90_10380"
     CDS             complement(7632..9110)
                     /locus_tag="PLS90_10380"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK02849.1"
                     /translation="MARRATWLWLLLIAAGASAQPIVMDGQFDDWCEAGMLHDDRGDV
                     PTTGTLDLGRLWAAHDREHLHLLLETGRETLMQDAESPIGNRISLFLDVDGSAETGLR
                     RDGLGVDVQIAFGEKIVTRHGPDGQTTQTTLNDVGLIMMPTHSSDTFEYQIALGGIGP
                     YADLATLPDPPLIRFFLQDLTTGDRLPNEGSIEYLLPAPEGEEVHEPIALERMAPSDI
                     RLLVHNVLKSNIVKNPEPFARYLRALRPDLICFQELEEWTVAQAVAFVTGVLPPGEGQ
                     QWHGAQQADVVTLSRWAPAQQAVVDENLICRFDVPTTGGAVRGLVLFNMHTPCCAFYE
                     ARQREHDRAIAAWRDILTGSPEALIAGNPDDAVILCGDFNMVGYARELYAVRDGDIFD
                     NERFGGDFAPGRAHGSLVSAPLRHTHSPQTYTWRWDPSEFGPGKLDYVLFSDDALKLK
                     RNFTLWTPEMPAEALAAAGLEPPDSPNASDHLVLVADFEVRE"
     gene            complement(9132..10196)
                     /gene="argC"
                     /locus_tag="PLS90_10385"
     CDS             complement(9132..10196)
                     /gene="argC"
                     /locus_tag="PLS90_10385"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPK02850.1"
                     /translation="MMRIGILGAKGYTAGELLRLLASHPQAEVTCLMARVDAPEPVAA
                     YFPALRGLYDLAIEPIDLERLIEQCDAAFLALPHTAAQEYAARLIEAGLKVVDLSADF
                     RFESVDLYEKTYKVEHKARELNSRIPYGLPELFRDELKQAPAIANPGCHTITAILALA
                     PLMRQGELVDLDRIVISSLTGVSGAGRKPSDITHFPECNESVNAYGVATHRHRPEIEE
                     QLSRLAGRKLRLVFTPHLIPMSRGILSTCTAPLRAPWTTAEAMALYREFYAGEPFIRM
                     LPDGEAPRTGAVMLSNFCDISVVVDEHARSVIAMAATDNLVKGASGQAIQNMNLLFGL
                     DETLGLIPAPRRPVAAPAAG"
BASE COUNT         1769 a         3830 c         3020 g         1783 t
ORIGIN      
        1 ccgccatgat caccatccgc ccgctcggcc gcaacaatgg gaccgcctgc atgagatcgg
       61 gctcgcgccg cacgtcgaac cagacgtgaa ccccctcggg cgcaagctgg cgcaccgcct
      121 cggccaggtc gcgttcccgg taccggatca cctcgtcggc gcccagctcg cggcagatct
      181 ccaccttcgc ctcgttgccc gccgacgtga tcacgcgggc ccccgccagt ttcgccatct
      241 ggatcacgac cgagccgacg ccccccgatc ccccggccac gaagatcgtc tcgttgcgct
      301 tgagcctggc cttctcgaac agccccaggt gcgccgtgat gcccaccagc gccagcgcgg
      361 ccacctcctc gtcgtcgacc cccgtcggcg tgcggtagac ccacgcctcg ttgaccgcca
      421 catactgcgc gaacgttccc tgccgcccga gcagcccctg gttggagccc cacacccgat
      481 cccccggctt gtagcgcgtt acttccgaac cggtcgcctc gaccgtcccg gcaaaatcgc
      541 agccgatgat gtacggcttg ggcagttcaa acgggatcat cccgctgcgg atgtacgtgt
      601 cgatcgggtt gaccgacacc gcccgcaccc gcacgagcac ctggtgcggt tcgggttccg
      661 gcgtcggcag atcgccatac tggatcacat cgggttcgcc cgtctggacg atataggcgg
      721 ctttcatccg aacaactcct cctcgatggg acgcgggcgc cggcggccgg ctcacagccc
      781 ccgcacggcc tccagcgcct tgccgtagtc cggttcgtgc gtcacctcgc tcacgatctc
      841 gcggtagacg accttgccct cggcgtccag cacgaagacc gaccgggcca gcagcgccag
      901 ttccttgatc acgaccccga acgcctcgcc gaaactgcgg ctcttgtagt ccgagacgca
      961 cgtgatgttg ttcgccttga acgattcgca gaaccgtttt tgcgcgaacg gcagatcgca
     1021 actgaccgtg acgacgcgca gtccgtgctc gcgcgcctgt tcgttgaact tctgcgtctg
     1081 gatctggcag acgcccgtgt cgagcgacgg gacgacgctg atgacgaccg gctggccctt
     1141 cagatccgcc aacgtcaggt ccttcagctc gccggtagtg accgtgaagt cgggggccgg
     1201 gtcgccgacc ttgacttccc gctggcccac cagcgtcatc ggggcgcctt tgaatgtgat
     1261 cgcgccgggg cgttccatgg ggggctcctc tgcttgcgat tcaaaagggg gaaggggcgc
     1321 gggctgcgga caacccgctc gggcgacagt gtagcagacg caagagacgt gtcaagccgg
     1381 ccaggaaatc gccgtggccg ccgccgctgg aacggcgtcc cgcggcggcg caccaacggc
     1441 ctttccgatc atgctttatt ccggccgctt cgacgacagg atccgcacct tgatccccag
     1501 catctggcac agcgccacca gctcgccgaa gatatcccca tacgccacgc agatgtggtt
     1561 gctcatgtaa tgcgccatca cggtttccat cgagcagccg acgtcggcca gcatcagcgg
     1621 ccactgcggc gtcgtccctt cccagaacag gtcgcgctgc ttcgccggca ccttcagcac
     1681 ctcgccgcgg ccgatgtcca tccacagcgc gttgttcttc agatatgcgc gggcccacgt
     1741 catcttgccg ggcaggctct cgccggtgaa cgtcccgccc gggatcggga agtagccctt
     1801 cggctggcga tacgaatgga ctcccttgag cgatttctca tcatgattga atgcgtaggc
     1861 cgaacaggag ccgctgttga gcaacaccca gatgaaccgg ttgccgacct tgccccccca
     1921 gcgcacgtcg tggaagttga ccgtctggtt cagccccttg cgcgccagca gccgcttcat
     1981 cagctccatc ggcaccagcg agccctggtc ggcttcggtc gcgcacatga tcggctggcc
     2041 gtcgccttcg gggcgggcgt tggagttgag cagaccctcg cagaaatcgc tcggcggcag
     2101 cagcgggatc agccccagct ggtactgcca ccccaggcag tcgcacttga actcgtcgag
     2161 caggtcaagc acggccagat acatcttgag ctgagtccgc gtggcatcct cggtaaagtc
     2221 cgagctgtcc tcgccccagt tcgtctcgcc ccagtggaat ttgacgccgc ggtccaggca
     2281 gaacttgagc gcgtcgtcca cgcgccgcgg gctgatccgc tgcacccggt cgacgatcca
     2341 ggcctgatcg accttgtgtt cgctgaatcc gatcttatgc agctcgcgca cgccgaagta
     2401 gccgttggtc atgcccatcg acgtatcgcc gagcatcatc gccagcacgc gccgatcgcg
     2461 aatcccggcg gccacggccg cggcgttgtc ggccgccgcg gccccgaccg gcgcggcgta
     2521 gtgcagctca tcctcgctgt aggcgatgcg gccggtcgag caccattcgt ccagccggtc
     2581 catgaacacc cggtcgcgcg tccagttgct cgcgtcggtc cagatccgcg aaaacggccg
     2641 gttgaccgcc tccaggcacg cccccgtgtt cagcagcccg accaggcccg gccactgccc
     2701 cgaaaagttc gacgccagca gcagcgggtt ctccttgccg ctcacgccgt ccacggtgtg
     2761 cggaccatag acccagtgca cgaaaatgcc gacgatcgga tcatcgatcg gaccgagctt
     2821 ttcgatcgcg tcgctcggcc gcgtcatgta acccttgatg cgatacggct ggcggcccag
     2881 cttgcgcagc gccgcctcca tctgcgccgt cgccttctcg atgttcggca acgcctgttt
     2941 gttcggcatc ggccggtagt cgcccggcca gaaaacggca accttccttg ctttcgccat
     3001 gatcggatcc tttgtgtgaa ttaatttccg ttgcgccggc gaggtgatcg gtcccccaca
     3061 ttccacaaaa cacctttgca tcctagtaac ccgccatcga agtcgcaacc ggaatccgtc
     3121 gcgcccccgc ggaccgaact cgagccggcc ctatggaatg cggcgtcagc gatcgcgcag
     3181 cggtcgtcga cgccgctctg gatccgataa ctcatgccga ccgcaatccc gattcggatc
     3241 ccgatcccga cacgcctcca cgtgctctcc ccctctcgcg gctttcgagc acctgtccgg
     3301 ccccttgcat cttacccggc gccatggaat actgcgctgg catgccgccc tggtccggcc
     3361 gcccagcacc ggccgacccg gcagaccgac agccctcggc acacacgacc cgccggcgcc
     3421 cacgacgcct tgcctccacc acgacaccgg attcaatcga cgttcaggcg gagcgcgttt
     3481 ccctgtgatg tgagcgatgg cgcaaacacc cgaaggccag gaggaaccgc atgagcggct
     3541 tcaacgagaa tcgctatgac cagatgcatt atcgccgctg cggccgcacc ggcctcaagc
     3601 tgcccgccgt ctcgctcggc ttgtggcaca atttcggcgc caaggccgag gccggcaaca
     3661 tccgggcgat gctgcgcacg gccttcgacc tgggcatcac gcatttcgat ctggcgaaca
     3721 attatggccc cccgcccggc gccgccgagg agaatttcgg ccgcgtcctc cacgacgact
     3781 tcgcccacca ccgcgacgag ctgatcatct cgaccaaggc cggctatcgc atgtggcccg
     3841 ggccgtacgg cgatttcggc agccgaaagt acctgatcgc cagcctggat cagtcgctca
     3901 agcgcatgaa gctggattac gtcgacatct tctatcacca ccggcccgac cccgaaacgc
     3961 cgctggagga aacgcttgag gccctcgaca tcatcgtgcg ccagggcaag gcgctctatg
     4021 tcggcgtcag cagctatccc ggcgcgttgc tcgtgcgcgc cgccgaaacg atgcgccagc
     4081 acaactggac cccgatcgcg attcaccagc cgcactacaa catgttcaac cgctggattg
     4141 aaaccgacct gctcgaccag accgaggaat acggcatcgg cgtcatcccg ttctgcccgc
     4201 tggcgcaggg ggtgctgacc aaccgctacc tcgatccgaa gaacccgatc ccgagcgatt
     4261 cgcgcgcggg aaatcccgac aatccgttca tgaccgccga atcggtcgaa cgggtcgtgg
     4321 gcaaggtgcg caagctcaac gtgatcgccg agcgccgcgg ccagtcgatg gcacagatgg
     4381 cgatcgcctg ggtcctgcgc gatccccgcg tcaccagcgc gctgatcggc gccagccgcc
     4441 ccagtcagat caccgactgc gtcgcggcgt tgaaccagct cggcttcacg caggacgagc
     4501 tgaccgaaat tgaccagatc ctcgccaatt gacggaattc acccagaacc gatgactcga
     4561 aaggcgtcaa ttcatgttgc atgatctcgc acggctgcgc gacggcgttt cccgccgcgt
     4621 ctccagttac gacacccgcg gcggcaacgc cgattcccat ttcatccgcg acggcgaaac
     4681 cttcgtcctc gccgacctca agggcgccgg catcatccgc cacatctggt tcaccatcct
     4741 cgccggcggc gattttccgc gccagatcgt cctgcgcatg tactgggacg acatggacta
     4801 tcccagcgtc gaggccccca tcggcgactt cttcggcatc ggccacgccc agctcgcctc
     4861 gctcaacacc gtccccttca gcgtctcagc caccgattac ctcggcccca aggccgcctt
     4921 caactgctac tggccgatgc cgttcgccac gcgcgcgcgc atcaccgtcc ataatgagac
     4981 cggcgccgac atccccgcct tttattccta catcgactac gatcagctcc cctcgattcc
     5041 cgacgacctg ggccggttcc atgcctggtg gaaccgcaac aacccctgca agccgcccag
     5101 gcacccgggc aaggacccgc aattcaacct caccgaccgg gacaactacc tgatcgccga
     5161 catccgcggg cgcggccatt tcgtcggttg caatctctcc gtccacaacc tctacggcgg
     5221 ctggtggggt gaaggcgacg acatgttcat ggtcgacggc cagaagtggc cccccgacct
     5281 gcacggcacc ggctcggagg actacttcgg ccatgcctgg ggcatgcagc ccgaaaacgc
     5341 cttcatctac aacggcgtct cctactacaa gaaggggacc gagccgggcg cgttcaacga
     5401 gcgcatcacc gtctaccgct atcacatcaa cgatccgatc atcttccaga agtcgctgcg
     5461 cgccagcatc gagcacggcc acgccaacga ccgctgcgac gactattgca gcacggccta
     5521 ctggtatcag accctgccgc acaagaaatt cccgccgttc cccaagatgg cggaccgcct
     5581 gccccgaccc gatgtgcagc tgacgatgca gcccctcgat gcgccgatca tgcccaccga
     5641 cctgcgtcat cgctcgggcc aggccttccc accgatgcgg gtgatcccgc ccccaccgcc
     5701 gcggaagaaa acgcggtagc ccaaaacaaa atccgtccga tccgtcggct ccgaccgatc
     5761 ggacggatgg ctctcattca gccggcggcc agctcgccga cggtcataac gagggatgaa
     5821 aatcgggagc cgccaacggc cgtttcgagc ggccgaggcc ccgccggatt gatcagccgg
     5881 ccagtcccgt cagggacggc agacttcagc ccgccacttc agtggcggga aacgggatgc
     5941 aagaagtcaa ggagtcccgt ggggacgaca gaaccgcgcc aatcgaccac gaaaacgtga
     6001 acctgatttt ctttgaatcg ttacgagtgc taatgcaacc cgaacgattc gggcaaacac
     6061 gagcgccttt tccccggcta cttcctgatc ccgccccgcg tcatcggcgc cgggtcgagc
     6121 agccgatcca gttcctccgc cttcaaatcg gtcatctcca gcgccacttc cttcaggctg
     6181 cgcccctcgg cgtatgcccg cttggcgatt ttcgccgcca gttcatagcc gatcaccgga
     6241 ttgagcgccg tcaccaggat cggattcttg tccagcagcc cggcgatccg ctcccggttg
     6301 acggtgaacc ccgccacggc cttgtcggcc agcacgcgcg ccgcgctgcc cagcaccgtg
     6361 atcgattcga gcaggttctt ggccagcgcc ggcaacatca cgttgagctg gaagttgccc
     6421 atctgattgc agatcgcgat cgccgtgtcg ttgccggtca cttgcgcgca ggccatcatc
     6481 atcgcctcgc agatgaccgg attgaccttg cccggcatga tcgacgagcc cggctgcagc
     6541 gacggcagcg cgatctcgcc cagccccgcg atcgggccgc tgttcatcag ccgcaggtcg
     6601 ttggcgatct tcatcagcgc cgacgcgctc gtcttgagct gcccgctcaa ttccgtcgcc
     6661 gagtccatcg ccgactgcgc ggcgaaatgg tcgggcgact cgacgaacgg cagcccggtc
     6721 gcctgcgcca gcgccgccgc cacgcgcgcg ccgaattccg gatgcgcgtt gatccccgtc
     6781 cccgtggcgg tgcccccctg cgccagttgc gccagccgcg gcagcgtccc ctccacgcgc
     6841 tcgatcgcct ggcgcacctg atacgcccaa ccgccgatct cctggcccag ccgcgtcggc
     6901 atcgcgtcca tcaggtgcgt gcgccccgtg gtcacgacgt cgtccagttc ccgcgcccgc
     6961 cgctccagcg tctcgcgcag tcgcttcagg cccggaagca gcacctcgct cgtctcgata
     7021 tacgccgcta cgtggatcgc tgtcgggatc acgtcgtttg acgactgccc catgttcaca
     7081 tggtcgttcg ggtggatctt cgtcttgccg tcgccgagtt gcatcgcgcg cgtcgcgatc
     7141 acctcgttgg cgttcatgtt cgtcgacgtc cccgacccgg tctggaacac gtcgatcgga
     7201 aagtgcccgt ccagcttgcc gtcgatcact tcctgcgccg cccggtcgat cagctccgcg
     7261 acggccggct ccagcagctt caactcccgg ttcacccgcg cgcagcactg cttcaccagc
     7321 cccagcgcgc gaatgaagac ccgcgggaac accgtcccgc tgattggaaa attctccacc
     7381 gcccgctgcg tctgcgccgc atacagcgcg tcggccggca ctttcacctc gccgagcgaa
     7441 tcgtgttcaa tccggtattc catcagctca ttgattcctt tccgtctgtc tctgcctcaa
     7501 aatctcgtcc ctcgtccctc gtcctcgtaa tcacccccaa cggccctccc ggaattcggc
     7561 tctcggctct cgtcccgcgt cctcggctct cgaaatcacc acttttcggc cgttcgcatt
     7621 acccactccc atcactcccg cacctcaaaa tcggccacca gcaccagatg atccgaggcg
     7681 ttcggtgaat ccggcggttc caacccagcc gccgccagcg cctcggccgg catctcggga
     7741 gtccacagcg tgaagttgcg cttgagcttc agcgcgtcat cgctgaacag aacgtaatcg
     7801 agcttgcccg gtccgaactc cgacgggtcc cagcgccagg tgtaagtctg cggcgagtgc
     7861 gtgtggcgca gcggcgcgct gaccagcgac ccatgggccc gccccggggc gaaatccccg
     7921 ccgaaccgct cgttgtcgaa aatgtcgccg tcgcgcaccg catacagctc gcgagcgtag
     7981 cccaccatgt tgaaatcccc gcagaggatc acggcgtcat cgggattgcc ggcgatcagc
     8041 gcttcgggcg accccgtcag gatatcgcgc caggcggcga tggcacggtc gtgctcgcgc
     8101 tggcgcgcct cgtaaaacgc gcagcacggc gtgtgcatgt tgaacagcac cagcccgcgc
     8161 acggcgcccc ccgtcgtcgg cacgtcgaac cggcagatca ggttttcgtc gaccacggcc
     8221 tgctgcgccg gcgcccagcg gctcagcgtc accacgtccg cctgctgcgc gccgtgccac
     8281 tgctgccctt ccccgggcgg caggacgccc gtgacaaacg ccacggcctg cgcgaccgtc
     8341 cactcctcca gttcctgaaa gcagatcaga tcgggccgca gcgcccgcag gtagcgcgcg
     8401 aatggctccg ggttcttcac gatgttcgac ttgagcacgt tgtgcaccag caggcggatg
     8461 tcgctcggcg ccatccgctc cagcgcaatc ggctcgtgga cttcctcccc ctccggcgcc
     8521 ggcagcagat actcgatgct cccctcgttc ggcagccggt caccggtcgt caggtcctgc
     8581 aggaagaacc ggatcagcgg cggatcgggc aacgtcgcca gatccgcgta cggaccgatc
     8641 cctcccagcg cgatctgata ctcgaacgta tcgctcgaat gcgtcggcat catgatcagg
     8701 ccgacgtcgt tgagcgtcgt ctgcgtggtc tggccgtcgg gcccgtggcg cgtgacgatt
     8761 ttttccccga aagcgatctg cacgtcgacg ccgagcccat cgcgccgcag accggtctcg
     8821 gcggagccat cgacatcgag gaacagactg atccgattcc cgatcggcga ctcggcgtcc
     8881 tgcatcagcg tctcgcggcc ggtctccagc agcagatgca aatgctcgcg gtcgtgcgcg
     8941 gcccacagcc gccccaggtc gagcgtcccg gtcgtcggga cgtcgccccg gtcgtcgtgc
     9001 agcatccccg cctcgcacca gtcgtcgaac tggccgtcca tcacgatcgg ctgcgccgat
     9061 gcgccggcgg cgatcagcag cagccagagc catgtcgcgc ggcgtgccat cggcgccgtc
     9121 tccttcggcg gtcaacccgc cgccggcgcc gcaaccggcc gccgcggcgc gggaatcagc
     9181 cccagcgtct cgtccaggcc gaacagcaga ttcatgttct gaatcgcctg gccgctggcg
     9241 cccttgacca gattgtcggt cgccgccatc gcgatcacgc tccgcgcgtg ttcgtcaaca
     9301 acgaccgaga tgtcgcagaa attgctcagc atcacggcgc cggtgcgcgg cgcctccccg
     9361 tccggcagca tgcggatgaa cggctcgccg gcgtagaact cgcgatacag cgccatcgcc
     9421 tcggccgtcg tccacggcgc gcgcagtggc gccgtgcagg tgctcagaat gccgcgcgac
     9481 atcgggatca ggtgcggcgt gaacaccagc cgcagcttcc gcccggcgag acggctgagc
     9541 tgctcctcga tttccggccg atgccgatgc gtcgcgaccc cgtaggcatt gaccgattcg
     9601 ttgcactcgg gaaagtgcgt aatgtcgctc ggcttccggc cggcgcccga cacccccgtc
     9661 aggctgctga tcacgatccg gtccaggtcc accagttcgc cctggcgcat caacggggcc
     9721 agcgccagaa tcgccgtgat cgtgtggcag ccggggttcg caatcgccgg cgcctgcttc
     9781 aattcgtcgc ggaaaagttc gggcaggccg taggggatgc ggctgttcag ctcgcgcgcc
     9841 ttgtgctcca ccttgtaggt cttctcatac aggtccaccg attcgaaccg gaagtcggcc
     9901 gacagatcga ccaccttcag gcccgcctcg atcagccgcg cggcgtattc ctgcgccgcc
     9961 gtgtggggca gcgccaggaa cgccgcgtcg cactgctcaa tcagccgttc aagatcgatc
    10021 ggctcaatcg ccagatcata gagaccgcgc agcgcgggaa agtaggccgc caccggctcg
    10081 ggcgcgtcca cccgcgccat caggcaggtc acctccgcct gcgggtgcga cgccagcaac
    10141 cgcagcagtt ccccggcggt gtaacccttg gcccctagga ttccgatgcg catcatcccc
    10201 tccccgttgc ctcccgcggc aagggatttc gccatagtct atgcgcggct gggttcaagt
    10261 tgcacgcgga aaaaaatcac aactcggcgg gggcgcgctc gccggccgcg gccggactcg
    10321 ccacgcggcg cgcgccgaac gtccgctcga tcagcacata gaacagcggc gcgaacagcg
    10381 tcacgagaaa cgttgaggtg ac
//
LOCUS       DAOPCO010000138        10365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6253_length_10365_cov_5.462755, whole genome shotgun sequence.
ACCESSION   DAOPCO010000138 DAOPCO010000000
VERSION     DAOPCO010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10365
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_10365_cov_5.462755"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1361
                     /locus_tag="PLS90_10390"
     CDS             201..1361
                     /locus_tag="PLS90_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02851.1"
                     /translation="MSPLPPRLLLAVCCWLPTLALRAQPVDVPTWRVGDTWAVREELR
                     AAFATETGTTELAGQADFLLRLGAIAERTSDWAGAHRCYVRQRQQGAVVGTGLTRLGT
                     LDVTIHINNGQVTGETWNRVGDLALVRETITLAGELRADFLQLRNVLLGTMTYSMQME
                     FNPPLEMMDFPMATIGETWQVATELRQSGRLTIDFVDNLFGLEDVDDPFEVILSREGT
                     ARLLGLETRGGRLNTRHVQAAGVFEAWWSPALAEAVEYQLAPVAFEGGGLNEYTRSVL
                     SASLQQSTAIGEPTFTPARPARGQPFTVEGTAPASSAVTARLAGAQATVNSNGAGRYS
                     LTLPSPSTDDATPASDDVGSFGVEITSGLARRVKTVQLQMPQTSAGGWTAYR"
     gene            1441..2322
                     /locus_tag="PLS90_10395"
     CDS             1441..2322
                     /locus_tag="PLS90_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK02852.1"
                     /translation="MAVQFPINAFLDSLDPDPAQALRLGRELGIHCYALRFFPSGRFP
                     AISDEDWRWIKATHDEGACGVESVSPGVNKGLFEASSIDAITDQIQETIDKACKIGVR
                     KLSLFTWRKTAHAAAPRGDTPSPDMPTAIPNLLYRMADAAENAGLRLTIENEPTQWGD
                     TGLAVGSIILAADHRWMRVLWDPANSLAARAAWGLREGDRALADPTGVLIDELRQLAD
                     RLEDVHVRDIRIASDGRWRWVPLGEGVIDWPRLLEALDQAGYRGGLTLEPHLQPKNER
                     ARHAVDYLRSIMQASAA"
     gene            2489..4654
                     /gene="malQ"
                     /locus_tag="PLS90_10400"
     CDS             2489..4654
                     /gene="malQ"
                     /locus_tag="PLS90_10400"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPK02853.1"
                     /translation="MRSEVPPPLRRLARYCGIKTWYFDNYGRRCHAPAETLLGILRSL
                     GVAIDDAEQAEAALADERRKRWDTPLEPVAVAWEGEPARIALRLPEDRAGDLVHWTLR
                     TEQGGEVGGTLAAEALPTRLRAELAGRRYLLKHLPLPDALPAGYHQLSIRLEPREPAA
                     SGRRSKRSRPAPAADALIIRAPGRAYDAPDVNPGNAWGVFLPLYALKSARNYGAGHFG
                     ELSRLADWVNSLGGSIVATLPLLAAFLDEPHEPSPYAPASRLFWNEFYLDMESIPELE
                     RCPEARRLLEAPEFAAAAERFRQERIVDYREQMRLRREPLAALARAFFREPLPTRRLA
                     FEQFLERNPAASDYAAFRAAVEKSGSWHGWTGSRRDGLLTSADYDAEARQYHLYVQFV
                     AEEQVNALAERMRRGGPGLYLDLPLGVHPDSYDVWRHREQFAHGVSGGAPPDEFFSRG
                     QNWGFAPLHPRRIRQTQYRYVIQTLRQLMRQAGMLRIDHVMWLHRLYWIPQGAAATAG
                     TYVHYPAEELFAILCLESHRHRTRLVGEDLGTVPEIVRRAMTRRGLARMYVMQFAVHG
                     AAGRPAIDPVPAGAVASLNTHDTPTFAAFWEALDVEDRLAMDLVDAAGAEELRRQRDA
                     IRHALRGQLALSPGEDERARACAAAMKHLAAGPASVVLVNLEDLWGEREPQNTPGTHL
                     ERVNWRRKGAVALEELLARDDWRALLSEIDALRRARRSG"
     gene            4773..7292
                     /locus_tag="PLS90_10405"
     CDS             4773..7292
                     /locus_tag="PLS90_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HPK02854.1"
                     /translation="MARYVCIHGHFYQPPRENAWLESIEQQDSAYPYHDWNARITAEC
                     YGPNAWARILNGKGQISEIINNYSRISFNLGPTLLAWMEDQAPEVYAAVLEADQASAR
                     RFGGHGSALAQVYNHMIMPLASARDKQTQVLWGIADFVHRFGRPPEGMWLAETAVDLD
                     TLEAMATYDIRFTILAPHQAARVRERGQSAWHEVNEGSLDTRHPYLCRLPSGRSIALF
                     FYNSSISHAVAFEGLLNKGEYLAGRLLSAFDRKSDAPQLVHIATDGETYGHHHRYGEM
                     ALAYALKAIEADPETRIANYAQFLELHPPTWEVEIHENTAWSCAHGVERWRSDCGCAT
                     GAHPGWNQAWRRPLREALDWLRDQAEPLFEQELGKILRDPWGARNDYIQVVLDRAPAT
                     LERFLQRHRRLALERSDVSRTIKLLELQRHAMLMYTSCGWFFDDISGIESVQILQYAG
                     RVIQLARELLDADLEKGFLQRLRRAHSNTDLVGDGAEIYRRWIKSGIVDLHKVGAHYA
                     LSSVFEDYPDMARVYAFTVERIEHQYHEAGRARLAIGRARITSDVTRESEQLVYAVLH
                     LGDQNISGGVRTDEGEAPYRTMRREISRAFARADMTEVLRQIDRAFRGHLYSIPSLFR
                     DEQRRIMGEVLREFLNEAERDYQRIYNRNAPLIRFVADLNMPLPRAFQVAAEFAINSL
                     LRQLFEAPDPDLERIDQLHEQALTHHVTLDAQTLEFALRGAIERQARALEQAPESLQA
                     LGRFDALMLSARQMPFEINLAVAQNIIHRLYQAQDGVWRRMIERAREDDDSAREWLEQ
                     FARLAEKLNLAVDRSAIPNGVHPTGGDDEEVPPPEEAEGGE"
     gene            complement(7851..>10365)
                     /gene="nifJ"
                     /locus_tag="PLS90_10410"
     CDS             complement(7851..>10365)
                     /gene="nifJ"
                     /locus_tag="PLS90_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPK02855.1"
                     /translation="VGGRYGLSSKEFSPAMVKAALDNLKLPRPKNHFVVGIEDDVTHA
                     SLPCDPHFSTEDPKVIRAMFFGLGSDGTVGANKNTIKIIGENTDFYAQGYFVYDSKKA
                     GAVTVSHLRFGPRPIRSTYLIAEANFVGCHQFHFLEKVDVLAKIVPGGTFLLNAPYAA
                     DEVWGRLPRPVQQTIIDKQLKFYVIDATRVAADSGMGRRINTVMQTCFFALSGVLPRD
                     EAIEQIKKSIRKTYGSKGDEVVRMNIQCVDNALGNLYEVTYPAQVTSTIERTAAVPAD
                     APPYVRDVLARIIAGDGDSLPVSAFPCDGTFPTDTARWEKRNLALEIPVWDPEVCIQC
                     GKCVMVCPHATIRMKVCDEANLAAAPPAFKSADARAPEFKGRKYTIQVAPEDCTGCEL
                     CVHVCPAKNRQDPTRKAINMEPQPLLRAQQAVNWDFFRALPEVDRAALSLAKVRTQQF
                     QQPLFEFSGACAGCGETPYIKLLSQLFGDRALIANATGCSSIYGGNLPTTPWARDPHG
                     RGPAWSNSLFEDNAEFGMGMRLAVDQLKQQATELLLKLTSVVGDELAAAILNAQQKTD
                     ADIQAQRARVVELKHKLAGLDTPEARRLLAIADNLVKRSVWIVGGDGWAYDIGYGGLD
                     HVLASGRNVNVLVLDTEVYSNTGGQMSKSTPRGAVAKFAAGGKPAGKKNLGLMAMSYG
                     NVYVAQVAMGARDEHTLQAFLEAEAFDGPSLIIAYAHCIAHGINMVEGLDQQKAAVDS
                     GQWLLFRYHPDRAAEGRNPLQIDSKEPRISVADYMMKENRFRMLTKSDPQRARELFDL
                     AADDARRRREVLTLLASQWASTEPAPAAKDTAEPVKTGS"
BASE COUNT         1588 a         3260 c         3811 g         1706 t
ORIGIN      
        1 ctgcagggtg cagaaccgtt tctcggcgga gtcaccggat ccaaagcggc gccgggggct
       61 gcgcggcggt tggcgggggg cgcgaagttg cttgtcttgc ggtcagccgt cccaccataa
      121 ttgccgccga gcatcaattg ccgccaaaca tccgccgcgc gcaaccccgg cgtccccgcc
      181 ggcgcccttg aggtccgacg atgagtccgc tgccgcctcg tctcctgctt gcggtctgct
      241 gctggctgcc gaccctggcg ctgcgggcgc aaccggtgga cgtgccgacc tggcgggtgg
      301 gcgacacctg ggccgtgcgc gaagagctgc gcgccgcgtt tgccacggaa acgggcacca
      361 ccgaactggc aggccaggcc gacttcctgt tgcggctcgg cgcgatcgcc gagcggacga
      421 gcgactgggc cggcgcgcac cgctgctacg tccgccagcg ccagcagggg gcggtcgtcg
      481 gcaccggcct gacgcgcctc ggcacgctcg acgtgacgat tcacatcaac aacggccagg
      541 ttaccggcga aacctggaac cgcgtcggcg atcttgcgct ggtgcgcgag acgatcacgc
      601 tggccgggga attgcgggcg gacttcctgc aactgcgcaa cgtcctgctg ggcacgatga
      661 cctacagcat gcagatggaa ttcaatccgc cgctcgaaat gatggatttc ccgatggcga
      721 cgatcggcga aacatggcaa gtcgccaccg aactgcgtca gtcagggcgc ctgacgatcg
      781 actttgtcga caacctcttc ggcctggagg acgtggatga tccgtttgaa gtcatcctga
      841 gccgggaagg gaccgcccgg ctactgggtc tcgaaacccg cggcggccgg ctcaacacgc
      901 gccatgttca ggccgcgggc gtgttcgaag cgtggtggtc gcccgcgctc gccgaggcgg
      961 tggagtatca actggcgccg gtcgcattcg aggggggcgg gctgaacgag tatacccggt
     1021 cggtcctgag cgcttcgctg caacagagca ccgcaatcgg cgagccgaca tttacgccgg
     1081 cgcggcccgc gcgcgggcag cccttcaccg tggaagggac ggccccggcc tcgtcggcgg
     1141 tgacggcgcg gctcgccggg gcgcaggcga cggtcaacag caacggggcg ggacgctaca
     1201 gcctgacgct gccgtcgccg tcgaccgacg acgcgacccc cgccagcgac gacgtcgggt
     1261 cgttcggggt ggagatcacc tcggggctgg cgcgccgggt caagacggtc caactccaga
     1321 tgccgcagac ctccgccggc ggctggacgg cctaccggta aattcgccgg ccgcgtcccg
     1381 cgtcttcctt cgccttggca ctaacttcgc gttgacctct gtttggaggc gcagcgcgcc
     1441 atggccgttc aattcccgat caacgccttt ctggacagcc tggaccccga cccggcgcaa
     1501 gccctccgtc tggggcgcga gctggggatt cattgctatg cgctgcggtt ttttccctcc
     1561 ggtcgattcc cggcgatctc cgacgaagac tggcggtgga tcaaggcgac gcatgacgag
     1621 ggggcgtgcg gcgtcgaaag cgtatcgccg ggggtgaaca aggggctgtt cgaggcgagc
     1681 tcgatcgacg cgatcacgga tcagatccag gagacgatcg acaaggcgtg caagattggg
     1741 gtgcgcaagc tgtcgctgtt cacttggcgc aagacggccc atgcggcggc gccgcgcggc
     1801 gacacgccga gccccgacat gcccaccgcc atcccgaatc tgctctaccg catggccgac
     1861 gccgccgaaa acgccgggct gcgcctgacg attgaaaacg aaccgaccca gtggggcgat
     1921 accgggctgg ccgtcggatc gatcatcctg gcggccgacc accgctggat gcgggtgctg
     1981 tgggatccgg cgaactcgct tgccgcgcgc gccgcgtggg ggttgcgcga gggggaccgg
     2041 gcgctggcgg accccaccgg cgtgctcatc gacgagctgc gccagcttgc cgaccgcctg
     2101 gaggatgttc atgtgcgcga catccggatc gcgtcggacg gccgctggcg ctgggtgccg
     2161 ctgggcgagg gggtgatcga ctggccgcgc ctgctggagg cgctggacca ggccggttac
     2221 cgcggcgggc tgacgctgga gccgcacctg cagccgaaaa acgagagggc gcgtcacgcc
     2281 gtcgattacc tccgatccat catgcaggcg agcgccgcct gagcgcgggg gcgaaccgcg
     2341 cgcgctggcc gttccgcatc gccgctttgc ggtttcgccg gtcgcatggc gtctcgcgct
     2401 tgcgaccgcc gcgcgccggt cgctaaactc actgttcgct gaacgccccg cgggcaagcg
     2461 agcccggcac acggatggac tggccttgat gcgttccgaa gttccgcccc cgctcaggcg
     2521 cctggcccgc tactgcggga tcaagacctg gtatttcgac aattacggcc ggcgttgcca
     2581 tgcgccggcc gagacgctgc tggggatcct gcgcagtctg ggcgtcgcga tcgacgacgc
     2641 cgagcaggcg gaggccgcgc tggccgacga gcggcgcaag cgctgggata cgccgctgga
     2701 gcccgtcgcc gtcgcctggg aaggggaacc ggcgcgcatc gcgctgcgcc tgcccgagga
     2761 ccgggccggc gacctggtgc actggacctt gcggactgaa caagggggcg aggtcggcgg
     2821 gacgctcgcc gccgaggcat tgcccacgcg cctgcgggcg gagctggccg gcaggcgata
     2881 cctgctcaag catctgccgt tgcccgacgc gctgcccgcg ggctatcacc agctcagcat
     2941 ccggctggag ccgcgggaac cggccgcgag cgggcggcgg agcaagcgca gccggccggc
     3001 gccagcggcc gacgcgctga tcatccgcgc cccgggccgg gcctacgacg cgcccgatgt
     3061 gaacccaggc aatgcctggg gcgtgttcct gccgctttac gccttgaaat ccgcgcgcaa
     3121 ttacggcgcg gggcattttg gtgaattgag ccggctggcc gactgggtga acagcctggg
     3181 cggcagcatc gtcgccacgt tgccgctgct cgcggcgttc ctggatgagc cgcacgagcc
     3241 cagcccgtac gccccagcca gccggttgtt ctggaacgaa ttctatctcg atatggagtc
     3301 gatcccggag ctggagcgat gccccgaggc gcggcggctg ctcgaggcgc cggaatttgc
     3361 ggcggcggcg gaacgatttc gccaggagcg gatcgtcgat taccgcgagc agatgcgtct
     3421 gcggcgggag ccgctggcgg cgctggcgcg cgcgttcttc cgcgaaccgc tgccgacgcg
     3481 ccggctggcg ttcgagcagt ttctggaacg caacccggcg gcttcggact atgcggcgtt
     3541 ccgcgcggcg gtcgaaaaat cgggctcgtg gcatggctgg accggcagtc gccgcgacgg
     3601 gttgctgacc tccgccgatt acgacgccga ggcgcgccag tatcatcttt acgtccagtt
     3661 cgtcgccgag gaacaggtca acgcgctggc cgagcggatg cgccgggggg ggcccggcct
     3721 ctacctcgac ctgccgctcg gcgtccaccc cgacagctat gatgtgtggc gccaccgcga
     3781 gcagttcgcc catggggtga gcgggggcgc gccgcccgac gaattctttt cgcgcgggca
     3841 gaactggggc ttcgccccgc tgcatccccg gcgcatccgc cagacgcagt atcgctacgt
     3901 gattcagacg ctgcgccagt tgatgcgcca ggccgggatg ctccgcatcg atcatgtgat
     3961 gtggctgcat cgactctact ggattccgca gggggccgcc gccacggccg gcacctacgt
     4021 gcactatccg gcggaggaat tgtttgcgat tctctgtctg gagtcgcatc gtcatcggac
     4081 gcgtctggtg ggcgaggatc tgggcaccgt gcctgagatc gtgcgccgcg cgatgacgcg
     4141 gcgcgggctg gcgcggatgt atgtcatgca gttcgccgtg catggcgcgg cgggccgacc
     4201 ggcgatcgat ccggttcccg ccggggccgt cgccagcctc aatacgcacg atacgccgac
     4261 gttcgccgcc ttctgggagg cgctcgacgt ggaggaccgg ctggcgatgg acctggtgga
     4321 cgccgcgggg gccgaggagc tgcggcgtca acgcgacgcg atccgccatg cgctccgggg
     4381 gcagttggcc ctgagcccgg gcgaggacga gcgggcgcgg gcgtgcgccg ccgcgatgaa
     4441 gcatctggcc gccgggcccg cctcggttgt cctggtcaac ctggaggacc tctggggcga
     4501 acgcgagccg cagaacacgc cggggacgca tctggagcgg gtcaactggc ggcgcaaggg
     4561 ggccgtcgcg ctcgaggagc tgctggcgcg cgacgactgg cgggcgctgc tgagtgaaat
     4621 cgacgcgctg cgccgcgccc gccgctccgg ctgacgcgcg cggggggcgc gcccgaaggc
     4681 ggaccgccat cgagcgcccg ttgcgcgcga ccgataagtt gttcaggccg gctgcgtgcc
     4741 gtcactcccc taaccccgga atccccaccg ccatggcgcg ctacgtctgc atccacggcc
     4801 atttttacca gcctccgcgc gagaacgcgt ggctcgagag catcgagcag caggattcgg
     4861 cctacccgta tcacgactgg aacgcgcgga tcacggccga gtgctacggc cccaacgcct
     4921 gggcgcgcat cctcaacggc aaggggcaga tctccgagat catcaacaac tactcgcgga
     4981 tcagcttcaa cctcgggccg acgctgcttg cctggatgga agatcaggcg cccgaagttt
     5041 acgcggcggt gctggaggcc gaccaggcga gcgcgcggcg gttcggcggt cacggttcgg
     5101 cgctcgctca ggtttacaat cacatgatca tgccgttggc cagcgcgcgg gacaaacaga
     5161 cgcaggtgct gtgggggatc gccgatttcg tgcaccggtt cggccggccg cccgaaggga
     5221 tgtggctggc ggagacggcc gtcgatctgg acacgctgga ggcaatggcc acctacgaca
     5281 tccgattcac gattctggcg ccgcaccagg ccgcccgagt ccgcgagcgg ggccagagcg
     5341 cgtggcacga ggtcaacgaa ggctcgctcg atacgcgtca tccctacctg tgccggctcc
     5401 cgtcggggcg gtcgatcgcg ctgttcttct acaacagctc gatctcgcac gccgtcgcct
     5461 tcgaagggct gctgaacaaa ggggaatatc tcgccgggcg tctgctgtcg gcgtttgacc
     5521 gcaagagcga cgccccgcag ttggtgcata tcgccaccga cggcgaaacc tacggccatc
     5581 atcaccgcta cggcgaaatg gccctggcct acgcgctcaa ggcgatcgaa gccgaccccg
     5641 agacgcgcat cgcgaactac gcgcagttcc tggagctgca cccgccgacc tgggaagtcg
     5701 agatccatga gaacaccgcg tggagctgcg cgcacggggt cgagcgctgg cggagcgatt
     5761 gcgggtgcgc caccggcgca caccccgggt ggaatcaggc gtggcgccgc ccgctgcgcg
     5821 aagcgctgga ctggctgcgc gaccaggccg agccgctgtt tgaacaggaa ctgggcaaga
     5881 tcctgcgcga cccgtggggc gcgcgcaacg actacatcca ggtcgtgctc gatcgcgcgc
     5941 ccgcgacgct cgagcggttt ctgcagcgcc accggcgcct cgcgctggag cggtccgatg
     6001 tgagccggac gatcaagctg ctcgagctgc agcgccacgc gatgctgatg tatacgagct
     6061 gcggctggtt cttcgacgac atctcgggca tcgagtcggt gcagatcctg caatacgccg
     6121 gccgggtgat ccagctggcg cgcgaactgc tcgacgccga tctggaaaag gggttcctcc
     6181 agcggctgcg gcgcgcgcac agcaacaccg atctcgtcgg cgacggcgcc gagatctatc
     6241 gccgctggat caagtcgggc atcgtcgacc tgcacaaggt cggcgcgcac tacgcgctca
     6301 gttcagtctt cgaagactat cccgacatgg cgcgggtgta tgccttcacc gtcgagcgga
     6361 tcgagcatca gtaccacgag gcggggcgcg cgcggctcgc gatcggccgc gcccgcatca
     6421 cctcggacgt gacgcgcgaa agcgagcaac tcgtctacgc ggtcctgcac ctgggcgatc
     6481 agaacatcag cggcggcgtg cgcaccgatg agggggaagc gccctaccga acgatgcggc
     6541 gcgagatcag ccgcgccttc gcgcgcgccg acatgacgga ggttctgcgc cagatcgacc
     6601 gggcgttccg cggccacctt tactccatcc cttcgctctt ccgggatgaa cagcggcgca
     6661 tcatgggcga ggtgctccgc gagtttctca acgaggccga gcgcgactac cagcgcatct
     6721 acaaccgcaa cgcgccgctg atacggttcg tcgccgatct gaacatgccg ctgccgcggg
     6781 cgtttcaggt tgccgccgag ttcgcgatca acagcctgct gcgccagctc ttcgaggcgc
     6841 ccgaccccga tctggagcgg atcgatcagc ttcacgaaca ggcattgacg catcacgtca
     6901 cgctcgacgc gcagacgctg gaattcgcgc tgcgcggggc gatcgaacgc caggcgcgcg
     6961 cgctggagca ggcgcccgaa tcgctccagg ccctcggccg cttcgacgcg ctcatgctgt
     7021 cggcgcgcca gatgccgttc gagatcaatc tggccgttgc gcagaacatc attcaccgcc
     7081 tgtaccaggc gcaagacggg gtgtggcgcc ggatgatcga gcgcgcgcgc gaggacgacg
     7141 acagcgcgcg cgagtggctg gagcagttcg cgcggctcgc cgagaagctc aatctggcgg
     7201 ttgatcgatc cgccattccc aatggcgtgc atcccaccgg cggggatgat gaggaggtcc
     7261 ccccgccgga ggaagcggag ggtggggagt gaactggatt gaccggtttt cgggctcgtg
     7321 gccgtgtttg tgattgtgat cgatttgggc ggttctgcgg tctctgatgg gagtggcggt
     7381 ggaccgattc gggcaaggtc gcggcgggcg gaatcagttg ttcagggacc gcgcttttca
     7441 tcgtcgatgt gaaccgccgg cgcaagagca gtcggataac gggatcattt ccggtcaccc
     7501 ctttgagggg ttttgggcgt tgggggcgga gacgggttcc ggcctgcggc cgtcaccctg
     7561 cgcgttattc cggtcgtccg ctccgcggac ttgggcatgc gccccgctgc agggacgggg
     7621 ggagggggcg ggcattcccc aagttgcact gcgcgccacc agggcttgtg tgattttcct
     7681 ctgccagcat gaattgaggc tggcagccag gactctattc agattgcgcc gcttcgaggc
     7741 gcatctgaca tccgtgccgg gatgtggagg acagaccggc gggagcgccg gtcccccacg
     7801 ggtgggggtg atgcccggtc aggccgcagg gagaagcgcg gccttgcttg ttacgagccg
     7861 gttttgaccg gctcggcggt gtccttcgcc gcgggggcgg gttcagtcga agcccactgc
     7921 gaggcgagga gggtgaggac ttcgcggcgg cggcgggcgt cgtcggcggc caggtcgaac
     7981 agttcgcggg cgcgttgtgg gtcgcttttg gtcagcatgc ggaagcggtt ttccttcatc
     8041 atgtaatcgg cgaccgagat gcgcggctcc ttggagtcga tctggagcgg attgcggccc
     8101 tcggcggcgc ggtcgggatg gtagcggaag aggagccact ggccgctgtc gacggcggcc
     8161 ttctgctggt cgaggccctc gaccatgttg atcccgtggg cgatgcagtg ggcgtaggcg
     8221 atgatgagcg aggggccgtc gaaggcctcg gcctcgagga aggcctgcag ggtgtgttcg
     8281 tcgcgggcgc ccatggcgac ctgggcgacg tagacgttgc cgtagctcat cgccatcagg
     8341 ccgaggtttt tcttgccggc cggtttgccg ccggcggcga acttggcgac ggcgccgcgc
     8401 ggggtggact tggacatctg gccgccggtg ttggagtaga cctcggtatc gaggacgagg
     8461 acattgacgt tgcggccgct ggcgaggacg tggtcgaggc cgccgtagcc gatgtcgtag
     8521 gcccagccgt cgccgccgac gatccagacg ctgcgcttga cgaggttgtc ggcgatggcg
     8581 agcaggcggc gcgcctcggg ggtgtcgagg ccggccagtt tgtgcttgag ctcgacgacg
     8641 cgggcgcgct gggcctggat gtcggcgtcg gttttctgct gggcgttgag gatcgcggcg
     8701 gcgagttcgt cgccgacgac ggaggtcagc ttgaggagca gttcggtggc ctgctgcttc
     8761 aactgatcga cggccaggcg catgcccatg ccgaactcgg cgttatcctc gaagagcgaa
     8821 ttggaccagg cgggtccgcg gccgtgggga tcgcgggccc agggggtggt ggggaggttg
     8881 ccgccgtaga ttgaggagca gccggtggcg ttggcgatga gcgcgcgatc gccgaagagc
     8941 tggctgagga gtttgatgta gggggtttcg ccgcagccgg cgcaggcgcc ggagaattcg
     9001 aagagcggct gctggaactg ctgggtgcgg accttggcga ggctgagcgc ggcgcggtcg
     9061 acctcgggga gggcgcggaa gaagtcccag ttgacggcct gctgggcgcg caggaggggc
     9121 tggggctcca tgttgatcgc cttgcgcgtg gggtcctggc ggttcttggc ggggcagacg
     9181 tggacgcaca gctcgcagcc ggtgcagtcc tcgggggcga cctgaatcgt gtatttgcgg
     9241 cccttgaatt cgggggcgcg ggcatcggcc gatttaaacg cggggggggc ggcggccagg
     9301 ttggcttcgt cgcagacttt catccggatc gtcgcgtggg ggcagaccat cacgcatttg
     9361 ccgcactgga tgcagacttc gggatcccag accgggatct cgagggcgag gttgcgcttt
     9421 tcccagcggg cggtgtcggt ggggaaggtg ccgtcgcagg ggaaggcgct gacggggagg
     9481 ctgtcgccgt cgccggcgat gatgcgggcg aggacgtcgc ggacgtaggg cggagcatcg
     9541 gccgggacgg cggcggtccg ctcgatggtg ctggtcacct gggccggata ggttacttcg
     9601 tagaggttgc ccagggcgtt gtcgacgcac tggatgttca tgcggacgac ttcgtcgccc
     9661 ttggaaccgt aagtcttgcg gatggatttc ttgatctgtt cgatcgcctc gtcgcgcggc
     9721 aggacgccgg agagggcgaa gaagcaggtc tgcatgacgg tattgatgcg gcggcccatg
     9781 ccgctgtcgg cggcgacgcg ggtggcgtcg atgacgtaga acttgagctg cttgtcgatg
     9841 atggtctgct ggaccggccg ggggaggcgg ccccagacct cgtcggcggc gtagggggcg
     9901 ttgagcagga aggtgccgcc ggggacgatt ttggcgagga catcgacctt ttccaggaag
     9961 tggaactggt ggcagccgac gaagttggct tcggcgatga ggtaggtgga ccggatcggc
    10021 cgcggaccga aacgaaggtg cgagacggtg acggcgccgg ccttcttcga gtcgtagacg
    10081 aagtagccct gggcgtagaa gtcggtgttt tcgccgatga tcttgatggt gttcttgttg
    10141 gcgccgacgg tgccgtcgga acccaggccg aagaacatcg cgcggatgac cttggggtct
    10201 tcggtggaga agtgggggtc gcaggggagg ctggcgtggg tgacgtcgtc ctcgatgccg
    10261 acgacgaaat ggtttttggg ccggggcagc ttgaggttgt cgagcgcggc cttgaccatg
    10321 gcgggggaga attctttgga ggagaggccg tagcggccgc cgacg
//
LOCUS       DAOPCO010000139        10332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6274_length_10332_cov_5.168532, whole genome shotgun sequence.
ACCESSION   DAOPCO010000139 DAOPCO010000000
VERSION     DAOPCO010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10332
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6274_length_10332_cov_5.168532"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PLS90_10415"
     CDS             <1..749
                     /locus_tag="PLS90_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02856.1"
                     /translation="HDYQSSDIYYNSATIYPYAEDVYLMFTANFSHFVHGCHTQLRAR
                     PNQIWEDYGLIEIQLAVSRDGIHWTRPERVPYIDIGRPDEWDRWLLTAGTGLIRKGDD
                     VLQFYSSSGHTHDSNIIRPEEYENAVEQKMGGTGIVRQRLDGFVSADVDHRGGFLETP
                     PVVFSGRKLRLNIDTGAVGLAQVELRDAEGRPLPGFELSECEPILGNFTDYYVNWRGG
                     SDVSALAGTPVKIHFKLNRAKLYGFQFTDE"
     gene            complement(1089..1180)
                     /locus_tag="PLS90_10420"
     tRNA            complement(1089..1180)
                     /locus_tag="PLS90_10420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1144..1146),aa:Ser,seq:cga)
     gene            1787..4657
                     /gene="tssH"
                     /locus_tag="PLS90_10425"
     CDS             1787..4657
                     /gene="tssH"
                     /locus_tag="PLS90_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HPK02857.1"
                     /translation="MLRIDLKAILKKLSPYCTRALEGAAGLCVSRTHYEVTVEHMLLR
                     LAEEPTGDIQWILRHFEIEPSEFIKALQRCIETLRSGNAGKPVFSPMLIEWFQEAWLV
                     SSVELGSPAIRSGGLLMALVANPGRTTLFNFTDALDRVRLEELRREFHDITWESPEAA
                     EEERLRSAAGGAAAPAAGAEGAHVPEGDTALGRFTIDFTAEARAGRIDPVFARDREIR
                     QMIDILARRRKNNPIITGEAGVGKTALVEGLALRIVKGDVPSVLKNVRLISLDMGLLQ
                     AGAGMKGEFENRLKQVISEVKASPTPIILFIDEAHTLIGAGGQAGGSDAANLLKPALA
                     RGELRTVAATTWGEYKKYFERDAALARRFQVVKADEPSVEDAITMMRGLREKYEESHG
                     VRILDSAVVAAAQLASRYISGRQLPDKSVDLIDTAAARVRVQLGCRPDRLDDCDRRVE
                     TLERELDAIERDKQAGRPTDETRIAELKEEIARTRDEGAALEERWQREMTVARRIQAL
                     RDRLEALRTGNADAAQEAEAAAPSGNGQPAAEAEAQADAAEGELAEEPLPETEAELAA
                     ELQQRLDELKQVQGRDPLVHVEVDPDIIGKVVSDWTGIPVGKMVSDEAATVLSLDARI
                     KERIKGQDHAIDAIVESIRAAKAGISNPNTPMGVFLLVGPSGTGKTETALTLADLLFG
                     GERFMVTINMSEFQEKHTVSRLVGSPPGYVGYGEGGVLTEAVRQRPYSVVLLDEVEKA
                     DPEVMNIFYQVFDKGMLADGEGRVIDFRNTIVMMTSNLETDTITSMCAAPLPPAPEDV
                     LTAIRPALSRHFKPALLARMRIVPYYTIDMEAMTRIVRLKLNGVGKRLAASHKMGFSY
                     DEAVIEQIAQRCTEVETGARNIDHILNGSLLPKISTEILQQMAVGPLPEHLNIGLDEN
                     GEFALQFSGNGAAAPEETAAPEPAMAGAIETSGPAQPAPEEE"
     gene            5102..7345
                     /gene="tssI"
                     /locus_tag="PLS90_10430"
     CDS             5102..7345
                     /gene="tssI"
                     /locus_tag="PLS90_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HPK02858.1"
                     /translation="MATQQEEQHWLYSDVSDYSFRAGDLDIQELRVIRFELHEAISEL
                     FHVRIELASLNQELAIDELVGRPGVLRLSHFNQEHERWVNGIVKLLDYTSSGSQYCRY
                     EAQLVPRVWLLGLRRQSRIFQDMTVPDIIKQVLTDAGIPADMFRMALTGNYGQRGYCV
                     QYRETDLDFIKRLAEEEGIFFFFEHTEETHVMVFGDAPSVHKPIAGKPEVPFRTPTDM
                     VEGKAFVSQFRYGRQVRTGSAMLRDFNFENPKQNLDAEKDSGKDTALEFYDYPGEYKV
                     KSEGETYVNVRLQEFRTFLKVARATSVERRLQPGYRFNLIEHPRQDLNAEYLVTRVRH
                     RGCHLPAVVQESGGVEVDEPTYQNEILAIPFSTPYRPPRVTPKPVVEGSQTAIVTGPS
                     GEEIYTDQYGRVKVKFHWDREGQHNEKSSCWIRVSQNWAGGKYGIMFLPRIGHEVIVD
                     FLEGDPDQPIITGRVYNNDEMPPYELPAHKTRSTIKSASSKDQEGSNEIRFEDLAGSE
                     QIFIHAQKDLHVRVLNERRVNIGADSHETVGANFNMHVEKDRSRKVKGKENIEVVEDK
                     CEKIGGNLSEEIGGSHKEKVGGTFQIDANKIILNATQEVVIKGPGGFVVINGGGTYVK
                     GNMVYINSGGAAVTEGPGTIIAPTAPAAADEAQYGEDTSYTAEKRPQKKIPKDKLEKK
                     SWIEIRLVDAKGKPVPGEAYELETSDGKTLVGTLDGKGRVRIEGIEPGTCMVCFPNRA
                     PDEWQRV"
     gene            7442..8962
                     /locus_tag="PLS90_10435"
     CDS             7442..8962
                     /locus_tag="PLS90_10435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HPK02859.1"
                     /translation="MSAPNYRLSPQADDGGQDGKGLVSWIKNRRKKNKKAAEPEAGPT
                     ASALSTAQATQQQIAPAATPSPASSSPAASTPPKPTPPSKPLPPTPESKASAKPTPPN
                     KPLPPTPPDPEPTPTDNPETISALAAPPDAKGNKPLHVVDGNDHISRIALQHGYSNWS
                     SLWALNPQLAAKRKNPHILFHGTRGKKNADVVKTPPLKEKEEAVATEQVHTFKVPVSD
                     LFLRLRILDEDLQPVADADYTLTVEGRNYTGKTAADGTIIQEITRGAQNGWLTINMKD
                     ESPEHTAKMEQAKKEGQAKTIASRNAESTPAETAATASSTSAAEVKARMAAAPQSKPS
                     TTKGLTTGQDRPALGGQIKFALRIGRLDPILEDAPDRWCVAGVQARLNNLGFESGPVD
                     GVKGPVTKAAIKRFQRRFKLVEDGIAGPVTQAKLKEVHDTNTPIPPLIQPATGQAGQD
                     AQATSTGTAQKTTGKAPIDGTSSAPGTPPSAPGSTPSSSASPSATPAGRPAAPAST"
     gene            9172..>10332
                     /locus_tag="PLS90_10440"
     CDS             9172..>10332
                     /locus_tag="PLS90_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02860.1"
                     /translation="MTKTVNPGPRDVGFVAPDPTDGTHFNTLVLRRPYRISLRVGDLD
                     ELFPDPINTNRGRAARLNAVGLFYRTLDEPKMEECLLENWKYFRESMHKPPLTNEKAE
                     EKLKEWLNSHILAKLPEEGKFEKIHLPGGFSVDQPWANTGLYPDYNAAHSLGGDHFAV
                     ETEYYTANDVMGKIPLLVKVERYDVKRGWLPAPGAMVYFQLQMPDDLPPFDPAKPCAE
                     QLNRPPLRESLQGPATGAPATANGAGPKLYSDTHITNYLVDNNPDPALRDPQANNVHV
                     DKGGKRGTPGSAAAGPVGGVIFEIKSRPGFHTPHPGRKMSHGELNVAVPAAPDGNDHK
                     HAVRVAANDEGEAGVIFMPSRCGGDRYKLRVYVGPPTLDSNGADEQAVAITTG"
BASE COUNT         2020 a         3507 c         3323 g         1482 t
ORIGIN      
        1 agcatgacta tcaatcgtct gacatatatt acaactcggc cacgatctac ccctatgccg
       61 aagacgtcta cctgatgttc acggccaatt tctcgcattt cgtccatggg tgccacaccc
      121 agttgcgggc gcgcccgaac cagatctggg aggattacgg cctgattgaa atccaactgg
      181 ccgtcagccg cgacggcatc cactggacgc ggcccgagcg ggtcccctac atcgatatcg
      241 gccggcccga tgaatgggac cgctggctgc tgacggccgg aacgggcctt atccgcaagg
      301 gcgacgacgt gctgcagttc tacagttcga gtggccatac gcacgactcc aacatcatcc
      361 gccccgagga atacgaaaac gccgtcgaac agaagatggg cggcacgggc attgtgcggc
      421 agcgcctgga cggcttcgtc tcggccgacg tcgatcatcg gggcggtttc ctggagaccc
      481 cgccggtcgt cttcagcggg cgaaagctgc ggctgaacat cgacaccggc gcggtgggtc
      541 tggcccaggt cgaactgcgc gacgccgagg gccggccgct ccccggcttc gaattgagcg
      601 agtgcgaacc gatcctgggc aatttcaccg attattatgt caattggcgg ggaggcagcg
      661 acgtctcggc cctggccggc acgccggtca aaatccactt caagctcaat cgcgccaaac
      721 tctacggctt ccagttcacc gacgaataag tcgtcgctcg tgcgaaatca gtatttcgga
      781 agggacatag gggacgtaag ggagggacgc cctccctcgc gccccacgcc tcatcgacgc
      841 gtctggaacc gggcagccgc agcaaatcga ggagcgtcgg cgagcggatc agccgcatcc
      901 cggtcttgcg ccggggcttg atcggcttga tcctcccctg ggctgcggca tgctgcaggc
      961 gctgacggaa gcgttggacc gcgggatcat cggtcgttga tgccgacatc accggagccg
     1021 atcagccgcg gatcaggaat cgcaacagcg cgttgagatc ggaaggatta aagtttgaaa
     1081 aaccaggatg gcggagaggg tgggattcga acccacgagg ggcggttaag cccctacccg
     1141 ctttcgaggc gggctccttc aaccactcgg acacctctcc gtgggcggaa cgcaagaggc
     1201 ggtcggccgt cgcattgtga actgcaagcg tccgctcgcc cggcgaccgc accccaatcc
     1261 cccaccttat acggcagagg ccgccgtcgc cgcaaccgat aatccggccc ccccgtcgga
     1321 tcaatgggtc agcccgctgc ctcgccgggg cgaggtggcg atcgcgttga gaaagtctgg
     1381 ttcctgcccg gaggtgctcc atgcggcgtc cgtcgacgcg tcccaccgcc tccccgcccc
     1441 ggcgcccgcg gcgcgccggc ggctaaaagg ttggcccgca ttcatttggc tctcgccaaa
     1501 accggaccgc tgatggtatg aagaaaggca gcaccccaag caagcggagc ttggccggat
     1561 cccggcacgg agggcgcggg ctctccgcga cggccaatga gacgaggaac aacggagaga
     1621 gattttgccc ggcggcggcg gggctgatat atcctgagca ggatgtagaa taaatcgccc
     1681 gcccccgtcg gccgtgtttc gctcccggcg cccccgatcc ccgcgcctca gccagcttcg
     1741 cgggaccgcc ccacggtccc accatttcgt ccgataggtg atctcgatgt tacggatcga
     1801 tctcaaagcc attctcaaga aactgagccc ctactgcacg cgcgccctcg aaggggccgc
     1861 cggcttgtgc gtctcgcgca cccactatga agtgacggtc gagcacatgc tgctgcggct
     1921 ggccgaggag cccaccggcg acatccagtg gatcctgcgc cacttcgaga ttgagccctc
     1981 ggagttcatc aaggcgctgc agcgctgcat cgaaacgctg cgcagcggca atgccggcaa
     2041 gccggtcttc tcgccgatgc tgatcgagtg gttccaggag gcgtggctcg tcagttccgt
     2101 ggaactgggg tcgccggcga tccggtcggg ggggctgctg atggcgctgg tggccaaccc
     2161 cggccgcacg acgctgttca atttcaccga tgcgctcgac cgggtccgcc tcgaggagct
     2221 gcgccgcgaa ttccacgaca tcacctggga gagcccggag gccgccgagg aggagcggct
     2281 gcggtcggcg gccggcggcg cggcggcgcc cgcggccggg gccgagggcg cccacgtccc
     2341 cgagggcgat accgccctgg gccggttcac gatcgacttc accgccgagg cccgcgccgg
     2401 gcggatcgac ccggtattcg cgcgcgaccg cgaaatccgc cagatgatcg acattctggc
     2461 gcggcggcgc aaaaacaacc cgatcatcac cggcgaagcg ggcgtcggca aaaccgctct
     2521 ggtggagggg ctcgcgctgc gcatcgtcaa gggcgacgtc ccctcggtcc tgaagaacgt
     2581 ccgcctgatc tcgctcgaca tggggctgct gcaagccggc gccggcatga agggcgaatt
     2641 cgagaaccgg ctcaagcagg tcatttccga ggtcaaggcc tcgcccacgc cgatcatcct
     2701 gttcatcgac gaggcgcaca cgctgatcgg cgccgggggg caggcggggg gctcggacgc
     2761 cgccaacctg ctcaagccgg cgctggcgcg cggggaactg cggaccgtcg ccgccaccac
     2821 gtggggggaa tacaagaagt attttgaacg cgatgccgcg ctcgcgcgcc ggttccaggt
     2881 cgtcaaggcc gacgagccga gcgtcgagga cgcgatcacg atgatgcgcg gcctgcgcga
     2941 gaagtatgag gaatcgcacg gcgtgcggat tctcgattcg gcggtcgtcg ccgctgccca
     3001 gctcgccagc cgctacatat cggggcgcca gttgcccgac aagagcgtcg acctgatcga
     3061 taccgcggcc gcccgcgtcc gcgtccagtt gggctgccgg cccgatcggc ttgatgactg
     3121 cgaccggcgc gtcgagaccc tggagcgcga gctggacgcg atcgagcgcg acaagcaggc
     3181 cggccgcccc accgatgaga cccgcatcgc cgagctgaag gaggagatcg cgcggacgcg
     3241 cgacgaaggc gccgcgctgg aggagcgctg gcaacgggag atgaccgtcg cccggcggat
     3301 ccaggcgctg cgcgaccggc tcgaggcgct gcgcaccggc aacgccgacg ccgcgcagga
     3361 agccgaggcg gcggcgccct cgggcaacgg ccaaccggct gccgaagctg aagcccaggc
     3421 cgatgcggcc gagggcgaac tcgcggagga accgcttccc gaaaccgagg ccgaactggc
     3481 cgcggagctg cagcagcgcc tcgatgagct gaagcaggtc caggggcgcg acccgctggt
     3541 tcatgtcgag gtcgatcccg atatcatcgg caaggttgtc tcggactgga ccggcatccc
     3601 ggtcggcaag atggtcagcg acgaggccgc cacggttctg tcgctcgacg cgcgcatcaa
     3661 ggaacgcatc aagggccagg accatgcgat cgacgccatt gtcgagtcga tccgcgccgc
     3721 caaggccggc atctcgaacc ccaacactcc gatgggcgtc ttcctgctgg tcggccccag
     3781 cggcaccggc aagaccgaga cggcgctgac gctggccgat ctgttgttcg gcggcgagcg
     3841 gttcatggtc acgatcaaca tgtcggaatt ccaggagaaa cacaccgtct cgcggctggt
     3901 cggctcgccg ccgggctacg tcggctacgg cgagggtggc gttctgacag aggccgtccg
     3961 ccagcgcccg tactccgtgg tgctgctgga tgaagtcgag aaggccgacc ccgaagtgat
     4021 gaacatcttc tatcaggtgt tcgacaaggg gatgctcgcc gacggcgagg ggcgggtgat
     4081 cgatttccgc aacacgatcg tgatgatgac gagcaacctg gagactgaca caatcacgtc
     4141 gatgtgcgcc gcgccgctgc ctcccgcgcc cgaagatgtg ctgacggcga tccggccggc
     4201 gctgtcgcgc cacttcaagc cggcgctcct tgcgcgcatg cgcatcgtgc cgtactacac
     4261 gatcgatatg gaggcgatga cgcgcatcgt ccggctgaag ctcaacgggg tgggcaagcg
     4321 gctggccgcc tcgcacaaga tgggcttcag ctatgatgag gcggtcatcg agcagatcgc
     4381 gcagcgctgc accgaagtcg aaacgggcgc gcgcaatatc gatcacatcc tcaacggctc
     4441 gctgctgccg aagatctcca ccgagatcct gcagcagatg gcggtcggcc cgctgcccga
     4501 gcatctgaac atcgggctgg acgagaacgg cgagttcgcg ctccagttca gcgggaatgg
     4561 ggccgcggcg ccggaggaga ccgccgcgcc cgaaccggcg atggccggcg cgatcgaaac
     4621 gtcgggcccg gcccagccag cgcccgagga ggaatgaaga cctcaaccgc ccgacccccc
     4681 ttcttgaagc aacaaggaga tcacgtcagg atctctggcc aggggacctg ttccgttgta
     4741 gaatccatca tcatcccgaa cgagctctgt ggacggccgt atccgatacg agacggagcg
     4801 cagatgattt ccaagggtga gcagcttccc cgcacgagcg gccccggccg gcggcggacc
     4861 gcggactcga gccgggggcg cgtccgccgg cccgcgcgac cacgccgcga aggcgccgtt
     4921 acggcaatcc gcatccgaaa cgcatttacc tgatctggtg gtggtttttc cgggccgtgt
     4981 catataaaga agacttggac ccgagacagg cagggtcggc cccccgtgct cgacccgttc
     5041 tccccatgcg gatggtctgt tcgaagagat ctgcgacacg acagacagga atggtgacag
     5101 catggctacg caacaggaag aacagcattg gctctactcg gacgtatcgg actactcgtt
     5161 ccgcgccggc gacctcgaca ttcaggaact gcgcgtcatt cgcttcgagc tgcatgaggc
     5221 gatcagcgag ctgttccatg tccgcatcga gctggccagt ctgaatcagg agctggcgat
     5281 cgacgaactg gttggccgcc ccggcgtcct gcggctctcg catttcaacc aggagcatga
     5341 gcgctgggtc aacgggatcg tcaagctgct ggattacacc agcagcggca gccagtattg
     5401 ccgctatgaa gcgcaactcg tcccgcgggt gtggctgctc ggcctgcgcc ggcagagccg
     5461 catctttcag gacatgaccg tccccgacat catcaagcag gtcctgaccg acgccggcat
     5521 cccggccgac atgttccgca tggcgctgac gggcaactac ggtcagcgcg gctactgcgt
     5581 ccagtaccgc gagaccgacc tggacttcat caagcgcctg gccgaggagg aaggcatctt
     5641 cttcttcttt gagcacaccg aagagaccca cgtgatggtg ttcggcgatg ccccgtcggt
     5701 gcacaaaccg atcgccggca agccggaggt tccgttccgc acgccgaccg acatggtcga
     5761 aggcaaggcg tttgtctccc agttccgcta tggccggcag gtccggaccg gctcggcaat
     5821 gctgcgcgac ttcaacttcg agaaccccaa gcagaacctc gacgccgaaa aggactcggg
     5881 caaggacacc gccctggagt tttacgacta tcccggcgaa tacaaggtca agtccgaggg
     5941 cgaaacctac gtcaatgtcc ggctgcagga attccgcacg ttcctgaagg tggcccgggc
     6001 gaccagcgtc gaacggcggc tgcagcccgg ctatcgcttc aatttgatcg agcatccgcg
     6061 ccaggacctg aacgccgagt atctggtgac gcgcgtccga caccgcggct gccacctgcc
     6121 ggccgtcgtg caggagtcgg gcggcgtcga ggtggatgaa ccgacgtatc agaacgagat
     6181 cctggcgatc ccgttcagca cgccgtaccg gccgccgcgc gtcacgccca agccggtcgt
     6241 ggagggttcg cagacggcca tcgtcaccgg cccttccggc gaggagatct acaccgacca
     6301 gtacggccgc gtgaaggtca agttccattg ggatcgcgag ggacagcaca acgagaagtc
     6361 gtcgtgctgg atccgcgtca gccagaactg ggcgggcggc aagtacggca tcatgttcct
     6421 gccgcggatc ggccacgagg tgatcgtcga tttcctcgag ggggatcccg accagccgat
     6481 catcaccggg cgcgtctaca acaacgacga aatgccgccc tacgagctcc cggcgcacaa
     6541 gacgcgcagc acgatcaagt cggccagttc caaggaccag gaggggagca acgagatccg
     6601 cttcgaggat ctggccggct ccgagcagat cttcatccac gcccagaagg acctgcacgt
     6661 gcgcgtgctc aacgagcggc gggtcaatat cggcgccgat tcacacgaaa cggtcggcgc
     6721 caacttcaac atgcatgtgg agaaggaccg ctcgcgcaag gtcaagggca aggaaaacat
     6781 cgaggttgtg gaggacaagt gcgaaaagat cggcggcaat ctgagcgagg aaatcggcgg
     6841 ctcgcacaag gagaaggtcg gcggcacgtt ccagatcgac gccaacaaga tcatcctgaa
     6901 cgccacgcag gaagtggtga tcaagggccc cggcgggttc gtcgtgatca acggcggcgg
     6961 cacctacgtg aagggcaaca tggtttacat taatagcggc ggggcggcgg tcaccgaagg
     7021 ccccggcacg atcatcgccc ccaccgcccc ggcggcggcc gacgaggcgc agtacggcga
     7081 ggacaccagc tacaccgcgg aaaagcgccc gcagaaaaag atccccaagg acaagctgga
     7141 gaagaaatcg tggattgaaa tccggctggt cgatgccaaa ggcaaaccgg tgccgggcga
     7201 agcctatgag ctggagacct cggacggcaa gacgctcgtc gggacgctcg acggtaaggg
     7261 ccgcgtccgg atcgagggga tcgagccggg gacgtgcatg gtgtgcttcc ccaatcgcgc
     7321 tcccgatgaa tggcagcgcg tttgagggcc gggcctcgca tgaaatcggc aagatggcag
     7381 gctccgcaac taaccccagc gacgtaacag ggcgcccagc ccgcggaggt aaggtctcga
     7441 gatgagtgct ccgaactatc gcctctcgcc ccaggcggac gacgggggcc aggacggcaa
     7501 gggactggtt tcctggatca agaaccggcg caagaagaac aagaaggcgg ccgagccgga
     7561 agcgggtccg accgcgtcgg cgttgtcgac ggcgcaagcg acccagcagc agatcgcgcc
     7621 cgccgccacg ccgtcgccgg ccagcagctc gccggccgcg tcgacgccgc ccaagccgac
     7681 accgcccagc aagccgctgc cgccgacgcc tgaatcgaag gcgagcgcca agccgacgcc
     7741 gcccaacaaa cccctgccgc cgacgccacc cgatccggaa ccgacgccga cggataatcc
     7801 cgagacgatc tccgccctgg ccgcgcctcc cgatgccaag ggcaacaagc ccctgcatgt
     7861 ggtcgatggc aacgaccata tctcgaggat tgccctgcag cacggctact ccaactggag
     7921 ttcactgtgg gcgctcaacc cgcaactggc cgccaagcgc aagaatcccc acatcctgtt
     7981 ccacggcacg cgcggcaaga aaaacgccga cgtcgtcaag acccccccgc tgaaggagaa
     8041 ggaggaagcg gtcgccaccg agcaggtcca tacgttcaag gttccggtct ccgatctgtt
     8101 cctccggctg cgcatcctgg acgaggatct ccagccggtc gccgacgccg actacaccct
     8161 gacggtcgag ggacggaact atacgggcaa gacggcggcc gacgggacga tcatccagga
     8221 gatcacgcgc ggggcgcaga acggctggct cacgatcaac atgaaggacg agtcgcccga
     8281 gcacaccgcc aagatggaac aggccaagaa ggaaggccag gccaagacga tcgcctcgcg
     8341 caatgccgag tcgacccccg ccgaaacggc cgccaccgcc tcctcaacca gcgcggcgga
     8401 ggtgaaggcg cgcatggccg ccgccccgca gagcaagccg tccaccacga agggcctgac
     8461 cacgggccag gatcggcccg ccctgggcgg acagatcaag ttcgcgctgc ggatcggccg
     8521 gctcgatccc attctggaag acgctcccga ccgctggtgc gtggccgggg tgcaggcgcg
     8581 cctcaataat ctcgggttcg aatcggggcc ggtcgacggg gtcaagggcc ccgtcaccaa
     8641 ggccgccatc aagcgttttc agcggcgttt caagctggtg gaggatggca ttgccggccc
     8701 cgtcacgcag gccaagctga aggaagtcca tgacacgaac accccgatac cgccgctgat
     8761 ccagccggca accggccagg ctgggcagga tgcccaggcg accagcaccg gcacggccca
     8821 gaagacgacc ggcaaggcgc cgatcgatgg gaccagttca gcccccggga ccccgccgtc
     8881 ggcgcccggc tccacgccgt catccagcgc ctcgccctcg gccacgccgg ctggccggcc
     8941 ggccgcgccc gcgtcgacct gacgggcgcc tgatcccgtc aacttgatcc gaaggatagc
     9001 gacaccatgg cacacgctcc cggttccacc ccaccctcgg ttcccccgac gccgcccagc
     9061 tcgccgtcgc catcggcggc caccgcgacg cccccgagcg cgccggccag caccccgtcg
     9121 gccgaggcaa ccccgacgtc caccccgccc aaaccgggct cacattccct gatgaccaag
     9181 acggtcaacc ccggcccgcg cgatgtcggc ttcgtcgccc ccgatccgac cgacgggacc
     9241 catttcaaca cgcttgtctt gcgccggccg tatcgcatca gcctgcgcgt cggcgacctc
     9301 gacgaactct tccccgatcc gatcaacacc aatcgcggcc gggccgcccg gctcaacgcc
     9361 gtcggcctgt tctatcgcac gctggacgaa cccaagatgg aggagtgcct gctcgagaac
     9421 tggaagtact tccgcgaatc gatgcacaag ccgccgctga ccaacgaaaa ggcggaggaa
     9481 aaactgaagg aatggctcaa ctcgcacatc ctcgccaagc tccccgagga gggcaagttc
     9541 gagaaaattc acctgccggg cggcttctcg gtcgatcagc cctgggccaa caccggcctg
     9601 tatccggact ataacgccgc ccattcgctc ggcggcgacc atttcgcggt ggagaccgaa
     9661 tactacacgg cgaacgacgt gatggggaag atcccgctgc tggtcaaagt ggagcgctac
     9721 gacgtcaagc gcgggtggct gcccgccccg ggcgcgatgg tctatttcca gctccagatg
     9781 cccgacgacc tgccgccgtt cgatccagcc aagccgtgcg ccgaacagct caatcgcccg
     9841 ccgctgcgcg aatcactcca ggggccggcc accggcgccc ccgccacggc caacggcgcc
     9901 ggacccaagc tctacagcga cacccacatc accaactatc tggtcgacaa caacccggac
     9961 cccgcgctgc gcgacccgca ggccaataat gtccatgtcg acaagggggg caagcgcggc
    10021 acgcccggct cggcggcggc gggccccgtc ggcggcgtga tcttcgagat caagtcgcgc
    10081 cccggcttcc acacgccgca ccccggccgc aagatgagcc acggggaact caacgtggcc
    10141 gtccccgccg ctcccgacgg caatgatcac aaacacgccg tccgcgtcgc cgccaacgac
    10201 gagggggagg ccggcgtgat cttcatgcct tcgcgctgcg gcggcgaccg ctacaaactg
    10261 cgcgtttacg tcggaccccc gaccctggac agcaatggtg ccgacgaaca ggcggtcgcc
    10321 atcaccaccg gc
//
LOCUS       DAOPCO010000140        10286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6311_length_10286_cov_4.960512, whole genome shotgun sequence.
ACCESSION   DAOPCO010000140 DAOPCO010000000
VERSION     DAOPCO010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6311_length_10286_cov_4.960512"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLS90_10445"
     CDS             complement(<1..183)
                     /locus_tag="PLS90_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02861.1"
                     /translation="MPDAYTLRHRQRRADWLLQEIDDALKRRPDPSEDELEQLARETG
                     VPVAALRGVISSFADFA"
     gene            complement(338..1978)
                     /locus_tag="PLS90_10450"
     CDS             complement(338..1978)
                     /locus_tag="PLS90_10450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02862.1"
                     /translation="MKRPLRELLAPGAVLLLAAALRFAALGRYELWLDENYSVVMAFA
                     EGGILRGALVDTNPPLYLYLLRGWMELFGITAGAVRSLSAVLGVAQLAVLGLWLHGFG
                     LSRRAVGWALLLGALAPLHVYYSQEARAYTLLYLLATLALWSFTRAARTGRRRDWLAH
                     GALIVAALYTHNIFLFAVPAFWAGAAILGFDRRQGRRLALAHAVAALLYLPWLLATLR
                     VQEQGALTWLAEGWAEANPLLLLPDSIQALAVGARLPRFVRMARVPTALAVLGFLWMI
                     AALVAAGLVGARRTAMETGREDRRRVLFLAAGTLIPLLLLLLYSLAIEPIYVVGRYDT
                     LVYPALLGVLGVGCARLAGRPARRGGGIAVSIGLLMVPLGITGLCVGLRLLPLAENQP
                     HQPQQARGKVLKDYVRPGDLIVCLGLEGAKILYQMRRHAIPGTLLTYPLATHNHLGYF
                     DPEGAATDPDLLARDAEAILEAFRAGDGAEEAPGRRLFLVLDPYAFRAPTPGGREAAY
                     TRIAETLIDALTAAGLESWQDGEFWRLTQGLGLRAYTE"
     gene            complement(2060..2542)
                     /gene="rpsG"
                     /locus_tag="PLS90_10455"
     CDS             complement(2060..2542)
                     /gene="rpsG"
                     /locus_tag="PLS90_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPK02863.1"
                     /translation="MSRKKKGSLVRDIAPDRRYNSVQVAKLINCVMHDGERSVSEKIV
                     YTAMERVGERLKNEEIQPLDVFTQAIENLKPLVEVKSRRVGGQNYQVPVEVRPERRQT
                     LAFRWLLAAARGRGERTMAERLANELVDAYNKTGSAVKKREDVHRMAEANKAFAHYRW
                     "
     gene            complement(2618..2989)
                     /gene="rpsL"
                     /locus_tag="PLS90_10460"
     CDS             complement(2618..2989)
                     /gene="rpsL"
                     /locus_tag="PLS90_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPK02864.1"
                     /translation="MPTINQLVRKSRNRVVKKNKVPALQGCPEKRGVCTRVYTATPKK
                     PNSALRKVARVRLTNGHEVTAYIPGEGHNLQEHSIVLIRGGRVKDLPGIRYHILRGVL
                     DCQGVANRKKSRSKYGTKRPK"
     gene            complement(3171..4367)
                     /locus_tag="PLS90_10465"
     CDS             complement(3171..4367)
                     /locus_tag="PLS90_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPK02865.1"
                     /translation="MNFSNELKPALPWLKVLFWLVTAAIGVMALRLLAPIIFWVLNVI
                     APFALALIVAYVFHPIVTFVQRKLKLGRIAGILILFVGILLALGGVLTLLAPVLYEQM
                     QLAYHALRENLPIIFDELTARYIDADTRAAIVAWFDTNLAQLDQQVSEVLSHLGGAAG
                     TVAEGSFSVLRQFIQVIYNIVAGIGGFVVLLIFVVIIAFYYLVDMDQIPGVIHTMLPE
                     KNRERWWRILKEGDRSVGGFLRGQLIVCLCVGTLTTVLLFAIGMRQYAVLVGFFAGLI
                     NFIPYLGPVTGFLPAMLWILFTGDLETWGDRGVRGAILLGGFAIIQMIEGFVLQPFVV
                     GRGASLHPLAVMFALAVGAQAGIGGMIIAVPVACFVKVLWVELYWRKRKAGDPDHAPG
                     PSPLAE"
     gene            complement(4492..5439)
                     /gene="fabD"
                     /locus_tag="PLS90_10470"
     CDS             complement(4492..5439)
                     /gene="fabD"
                     /locus_tag="PLS90_10470"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPK02866.1"
                     /translation="MMKTIAMIFPGQGSQAVGMGRALAEQSAEARALFDRADAVLGYA
                     LSKLCFEGPEDELKLTVHTQPALYVSSAAALAVLRAAGIEPAMVAGHSLGEYSALYAA
                     GVFDFETGLRLVEVRGKAMYAAGQARPGTMAALLNLEDDVVKSICEQASAKGVVVPAN
                     WNSPGQIVVSGDPAAVEEAVRLAQEAGSRRSVRLPVSGAFHSPLVRPAVDAMKEALAG
                     VALNEPRCRFVANVSAQFVSRPEAIRQGLEEQIVSCVRWAESVETMAAAGAEVFVEVG
                     SGKVLTGLLRRINKELRGVNFGVPEDLAGVKAAVEGGGE"
     gene            5555..6373
                     /locus_tag="PLS90_10475"
     CDS             5555..6373
                     /locus_tag="PLS90_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02867.1"
                     /translation="MPSDRQIHPRPISAPLLTLLALAVLAGCARGPKHEGDAAADWIA
                     HNLVKLMGGEDALADLRFLSFDFVVRENGAVTLRRSHLWDRRKGYHRLEELRGNQLHV
                     TLMDVDTREGASFVQTEDGFVDTTTPESLAAAFDSFTHDTHWLLAATRLLEPGTKLEY
                     LGQQPVADRSFETLKLSFIPGDELTPQDAYWFHLSPEDGLPWGWSVAPDRPDATPTVF
                     QWTEWQEVGDFKLPVRFEEQGAARQIVIDKLYSPADVRTIVFAQPDAPTGVIPR"
     gene            6562..7515
                     /gene="truB"
                     /locus_tag="PLS90_10480"
     CDS             6562..7515
                     /gene="truB"
                     /locus_tag="PLS90_10480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPK02868.1"
                     /translation="MTSKTGPSGILLIDKPRGMTSHDVVAVVRRLAGTRAVGHAGTLD
                     PMAGGLLVLCLGRATRVAQFLSGLDKVYTGTIVLGAISSTYDAEGDIREQDRPVPEEE
                     SVVRAAMARQLGPGVQLPPPYSAVKVRGRKLYEYARAGEPVPRKPRNVHIWRFDLIQY
                     DPPQARFEARVGSGTYIRSMAHDLGLALGCGAYLSRLRRTRVGAFGVDQAIPLAELRA
                     DPDLLPARLLGVTEALTHLPKVTIQPEAERLVATGRSFTTGHILEFEGILNPGQPVLV
                     IDTQGRALSIVRPEPDEQLTETPVLGATAMIFRPLRVLAPA"
     gene            7547..9163
                     /locus_tag="PLS90_10485"
     CDS             7547..9163
                     /locus_tag="PLS90_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02869.1"
                     /translation="MAESHPATVPRAASARPARWLSGAMLALAIALLAWLSLGLAPRW
                     RASLDYPFQLDAEEGFLLNQAVRLAQGRGIYTPIDQPPYLVGNYPPLYPALYALVHGS
                     RARPASLPVGRAIVQAATVLSALLLALIAWRATRRPAPLLLAPVLFLAAYEVHHWSAF
                     ARVDLPALALTLAGLALFLEGRRPAHLAASALLFVAAACTRQTAVMAPLACCLLLAWT
                     DRRGLAWFAGAWVGGGLLALAALQAATRGEFWRHVVAYNVNSFDWSAWRAVMRNEVWF
                     FHRWILLACAGIALGPAVASFSARRRSAVPAAKAATAPPPRASAAWSVAGLFALLTTA
                     FSLPAMAKIGSAPNYLLEPLAAWALVIALALGHALDGRSNAGTGGRSGARSLAALLLA
                     ACLLLHAGHLVRLRPVLHSSRNPTPADREALRPIVADLRAQPGPVYAEEPVLALLAGK
                     EVLLQPFILSQLAREGRWRDAGWVAMLEDRTLTRLVVNEDLSAAGPEQRYRRYTATTA
                     AAVRENYRPAGAISLPGLATYYVWEPKMAE"
     gene            complement(9290..10066)
                     /locus_tag="PLS90_10490"
     CDS             complement(9290..10066)
                     /locus_tag="PLS90_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02870.1"
                     /translation="MRWAMLFLLAVTLVIAAPAPAAADNFSIRIGYDSGPVFYSPPVY
                     REYRSYSSFGFPTYYREEVYVSPWICPPSTVFIYDRPLYRPRYLSDYRYYSVRRPYTN
                     HYVVRPIYRDRHDHDRDWDRRDWDRYDRNRDRDHRDWDRSDRDRRRDEQVRWDRDRDR
                     DPGRRLIDRNDRTERRITERRESDWTRSSALSRNNDRVWRADSRSDSIRKSLIDDRGR
                     SAIRSDFSRRDTDRLGSRSSLGSRTEFNRSSLRDRGRRSN"
BASE COUNT         1624 a         3877 c         3142 g         1643 t
ORIGIN      
        1 cgcgaagtcg gcaaacgagc tgatgacgcc gcgcagggcg gcgacgggga cgccggtctc
       61 gcgggcgagt tgctccagtt cgtcctcgct cgggtcgggg cggcgtttga gcgcgtcgtc
      121 gatctcctgc aggagccagt cggcgcggcg ctggcggtgg cgaagggtgt aggcgtcggg
      181 catggggcaa ccgtctcccc cggcaaatcg gcggggtata ttctgataac tggatgcaaa
      241 tataacattt atcggctccg ccgcaatgcc tttttttcgc tcgtcgcgct tcgtgccccg
      301 attccgaatt ccgatcctga ttcgaccgcg tcggcggtta ctccgtatac gcccggagcc
      361 ccaacccctg cgttagacgc cagaattccc catcctgcca ggactccaac ccggcggcag
      421 tcaacgcatc gatcagcgtc tcggcgatcc gcgtgtaggc cgcttcgcgc ccgccgggcg
      481 tcggcgcgcg gaacgcatag ggatcaagca ccaggaacag gcgccgcccc ggtgcttcct
      541 ccgctccgtc tcccgcgcgg aaggcctcca gaatcgcctc ggcgtcgcgc gccagcaaat
      601 ccggatcggt cgccgccccc tccggatcga aataccccag gtggttgtgc gtggcgagcg
      661 ggtaggtcag cagcgtccca ggaatcgcgt ggcgccgcat ctgatacaga atcttcgccc
      721 cctccagccc gaggcagacg atcaggtcgc ccgggcgcac gtaatccttg agcactttcc
      781 cccgcgcctg ctgcggctgg tgcggctggt tttccgccag cggcagcagc ctgagcccga
      841 cgcacagccc cgtgatgccg agcggaacca tcagcagccc aatgctgacc gcgatcccgc
      901 cgccccgtct cgccggccgc cccgccagcc gcgcgcaccc gacgcccagc acccccagca
      961 gcgccgggta gaccagcgtg tcgtagcgac cgacgacgta gatcggctcg atggcgaggg
     1021 agtagagcag cagcagcaac agcgggatga gcgtgccggc cgccagaaac aggacgcgcc
     1081 ggcggtcttc ccggccggtc tccatcgcgg tgcgccgcgc ccccaccagc ccggccgcca
     1141 ccagcgccgc gatcatccag aggaacccca gcaccgccag cgccgtgggg actcgcgcca
     1201 tccggacgaa ccgcggcagc cgggccccca cggccagcgc ctggatcgaa tcgggcagca
     1261 gcagcagcgg attggcctcg gcccagccct cggccaacca ggtcagcgcc ccctgctcct
     1321 gcacccgcaa cgtcgccagc agccacggca ggtagagcag cgccgctacc gcgtgcgcca
     1381 gcgcgagccg tcgcccttgc cggcgatcga acccgaggat cgctgccccc gcccagaatg
     1441 ccggcacggc aaacaggaaa atgttgtgcg tataaagcgc cgccacgatc agcgccccgt
     1501 gcgccagcca gtcgcgccgg cgccccgtcc gcgccgcgcg cgtgaaactc cacagcgcca
     1561 gcgtcgccag caggtagagc agcgtgtagg cgcgcgcctc ctgggaataa tagacatgca
     1621 gcggcgcgag cgcgcccaac agcagcgccc agccgacggc acgccgcgac aagccgaagc
     1681 cgtgcagcca cagccccagc accgccagtt gcgccacgcc cagcaccgcc gagagcgatc
     1741 gcaccgcccc ggccgtgatg ccgaacagtt ccatccagcc gcgcagcagg tagagataca
     1801 gcggcggatt ggtatcgacc agcgccccgc gcaggatccc cccctcggcg aaggccatca
     1861 cgacggagta attctcatcg agccacagct cgtagcggcc gagcgccgcg aaccgcaagg
     1921 ccgccgccag caacagcacg gcgccgggcg ccaggagctc gcgtaacggc cgtttcatcc
     1981 aggaccgcca caaatgcaga accggcctgg catgtgcccg ggccggttcc atttcaagtc
     2041 gaagggttga aacgcgcggc taccagcgat agtgcgcaaa cgccttgttc gcttcggcca
     2101 tgcggtgcac gtcctcgcgc tttttgaccg ccgagccggt cttgttgtag gcatccacca
     2161 gttcattggc caagcgctcg gccatcgtcc gctcgccgcg gccgcgcgcc gccgccagca
     2221 gccagcggaa cgccagcgtc tggcgccgtt cgggccggac ctccaccgga acctggtagt
     2281 tctgaccgcc cacgcgccgg cttttgacct ccaccagcgg cttgagattc tcgatcgcct
     2341 gggtgaacac atcgagcggc tggatttcct cgttcttcag ccgttcgccc acgcgctcca
     2401 tcgccgtgta gacgatcttt tccgaaacgc tgcgttcgcc gtcgtgcatc acgcagttga
     2461 tcagcttggc cacctgaacg ctgttgtaac gccgatccgg cgcgatgtcg cgaacgagac
     2521 tgcccttctt tttccggctc atgccctgtt aacccctgga ctttccgcgt tggatgcgcc
     2581 gccttcggcg gcggcaaaac tcaatcgttc cggcccttta tttcggccgc ttcgtcccat
     2641 acttcgaccg gcttttctta cggttggcga ccccctggca gtccagcacg ccgcgcagaa
     2701 tgtggtagcg gatgcccggc aggtccttga cgcggccgcc gcggatcagc acgatcgagt
     2761 gctcctgcag gttgtgcccc tcgcccggga tgtaggccgt cacttcgtgg ccgttggtca
     2821 gccgcacgcg cgccaccttg cgcagcgccg agttgggctt cttcggcgtc gccgtataca
     2881 cgcgcgtgca gaccccccgc ttttccgggc acccctgcaa cgccggcacc ttgtttttct
     2941 ttacgacgcg gttgcgcgat ttgcggacca gttgattgat cgtcggcatg cctgaacctg
     3001 ttccgattga ctgtggaggg gaattccccg cttctgcgtc gccggtggat gaagacgcgt
     3061 agtccgccct ccgtacccac cacggcccgc aaaacgcgct atttaagcgg cccggcccgt
     3121 cgaagtcaag gccaaaaagg ctcccggcca acgcgatggc tgcgccggcc ctactccgcc
     3181 agcggcgacg ggccgggcgc gtgatcggga tcgcccgcct tgcgtttgcg ccagtagagc
     3241 tccacccaca gcaccttgac gaagcacgcc accggcacgg cgatgatcat cccgccgatc
     3301 cccgcctgcg cccccaccgc cagcgcgaac atcaccgcca gcggatgcag gctcgccccg
     3361 cgccccacca cgaacggctg caagacgaaa ccctcgatca tctggatgat cgcgaacccg
     3421 cccagcagaa tcgcgccccg cacgccgcgg tccccccagg tttccaggtc gccggtgaag
     3481 aggatccaca gcatcgccgg cagaaaaccg gtcaccggtc cgaggtacgg aatgaagttg
     3541 atcagcccgg cgaaaaatcc gacgagcacc gcatattgcc gcatgccgat cgcaaacagc
     3601 agcaccgtcg tcagcgtgcc gacgcagagg cagacgatga gctgaccgcg cagaaacccg
     3661 cccaccgatc ggtcgccctc cttcaggatc cgccaccagc gttcgcggtt cttctcgggc
     3721 agcatcgtgt ggatcacgcc ggggatctgg tccatgtcga ccagatagta gaacgcgatg
     3781 atgacgacga agatcagcag gacgacgaag ccgccgatgc ccgccacgat gttgtaaatc
     3841 acctggatga actggcgcag gacggagaac gagccttcgg ccaccgtgcc cgccgcgccg
     3901 ccaagatgcg agagcacctc gctgacctgc tggtcgagct gcgccaggtt ggtgtcgaac
     3961 caggcgacga tcgcggcgcg cgtgtccgca tcaatgtagc gcgcggtcag ctcgtcgaaa
     4021 atgatcggca gattctcccg cagcgcgtga tacgccagtt gcatctgctc gtaaagcacc
     4081 ggcgccagca gcgtcagcac gccgcccagc gccagcagga tgccgacgaa caggatcagg
     4141 atccccgcga tccgtcccag cttcagcttg cgctggacga acgtcacgat cggatgaaag
     4201 acgtaggcga cgatcagcgc cagcgcgaac ggcgcgatca cattgagcac ccagaagatg
     4261 atcggcgcca gcagccgcag cgccatcacg ccgatcgccg ccgtcaccag ccagaacagc
     4321 accttgagcc acggcagggc cggcttgagt tcgttggaga agttcatgcg gattccttcc
     4381 cgcgtcggct gggtgcggcg ggcgcgacgt ctggccggtg tgcgccagat tagttaataa
     4441 ccggctcgaa ttcacccaaa accggacaag cccggcaacg cctcgtccct cctattcgcc
     4501 ccctccctct accgccgcct tcaccccggc caggtcctcc ggcacgccga agttcacccc
     4561 ccgcagctct ttgttgatcc gccgcagcaa gcccgtcagc accttgcccg accctacctc
     4621 gacaaacacc tccgcccccg cggcggccat cgtctccacc gattcggccc agcgcacgca
     4681 cgagacgatc tgctcctcca gcccctggcg gatcgcctcg ggccggctga cgaactgcgc
     4741 gctgacgttc gccacaaacc tgcaccgcgg ctcgttcagc gccacgccgg ccagcgcctc
     4801 cttcatcgcg tcgaccgccg gccgcaccag cggcgaatga aacgcgccgc tgaccggcag
     4861 ccgcaccgac cgccgcgagc ccgcttcctg cgccagccgc accgcctcct ccaccgccgc
     4921 cgggtcgccg ctgacgacaa tctggcccgg tgaattccag ttggccggca ccaccacccc
     4981 cttggcgctc gcctgctcgc agatcgactt gaccacgtcg tcctccaggt tgaggagcgc
     5041 cgccatcgtt ccgggccgcg cctggccggc cgcatacatc gccttgccgc gcacctccac
     5101 cagccgcagg ccggtctcga aatcgaacac ccccgccgcg tagagcgccg agtattcccc
     5161 cagcgagtgg ccggccacca tcgccggctc gatccccgcg gcgcgcagca ccgccagcgc
     5221 cgccgccgag ctcacataca gcgccggctg cgtgtggacc gtcagcttca attcatcctc
     5281 cggcccctcg aagcacagct tgctgagcgc gtagcccagc accgcgtcgg cgcggtcgaa
     5341 cagcgcccgc gcctcggcgg actgctcggc cagcgcgcgg cccatgccga cggcttgcga
     5401 tccctggccc ggaaaaatca tggcgattgt tttcatcatg cgcttgtgct ccaatgcccc
     5461 gaaaatcccc agaaatggga gtggcggcgc ccgttcgcgg cgcgtcgcgc cgccgccggg
     5521 cggcttgccc gcaaatcccc cacagggtga caaaatgccc agcgaccgtc aaatacatcc
     5581 gcgtccgatt tccgcccccc tactcaccct cctcgcgctg gcggtcctgg ccggctgcgc
     5641 ccgcgggccc aaacacgaag gggatgccgc tgccgactgg atcgcccata acctcgtcaa
     5701 gctgatgggg ggcgaagacg ccctggcgga cctgcgcttc ctgagcttcg actttgtcgt
     5761 ccgcgagaac ggcgccgtca ccctccgccg ctcgcatctg tgggaccggc gcaagggcta
     5821 tcaccggctg gaggaactgc gcggcaacca gctgcacgtc acgctgatgg acgtcgatac
     5881 gcgcgagggg gcctcgttcg tccagaccga ggacggcttt gtcgacacca ccacgcccga
     5941 aagcctcgcc gccgccttcg acagtttcac tcacgacacc cactggctgc tggcggcgac
     6001 ccggctgctt gaaccgggga cgaaactcga atacctcggc cagcagccgg ttgccgaccg
     6061 ctccttcgaa acgctcaagc tgagtttcat ccccggcgac gagctcacgc cgcaagacgc
     6121 ctactggttc cacctctcgc ccgaggacgg gctgccgtgg ggctggtcgg tggcgcccga
     6181 tcggcccgac gcgacgccga cggtctttca gtggaccgaa tggcaggaag tcggcgattt
     6241 caaactgccg gtgcggtttg aagagcaggg cgccgcgcgc cagatcgtga tcgataagct
     6301 ctattcgccc gccgacgtgc gcacgatcgt cttcgcccag cccgacgccc ccaccggcgt
     6361 catcccccgc tgatctaccg acaattttcc gttcctcgtc cgcggctctc gaaatggcct
     6421 ttcagctttt tcgccccccc ttccagcccc gccgaccaaa atcgttggca tgcgggcgcg
     6481 gcctgggggt agcttgatgg gttgggacgc gcgcccaccg attcgcccga tcgacacccc
     6541 acgcgaaaga cgtaactcgc cgtgacttcc aagaccggcc ccagcggcat cctcctgatc
     6601 gacaagccgc ggggcatgac ctctcacgat gttgttgcag tggtgcgccg ccttgccggc
     6661 acgcgtgccg tcggccacgc cggcacgctc gatccgatgg ccggcggcct gctggtcctc
     6721 tgcctcggcc gggcgacgcg cgtggcccag ttcctcagcg gcctggacaa ggtctacacg
     6781 ggcacgatcg tcctgggggc gatcagctcg acctacgacg ccgaagggga tatccgcgag
     6841 caggaccggc ccgtccccga ggaggaatcg gtcgtgcgcg cggcgatggc gcgccagctc
     6901 ggccccggcg tccaactgcc gcccccctac tcggccgtca aggtccgcgg ccgcaaactg
     6961 tatgaatacg cccgcgccgg cgagcccgtt ccgcgcaaac cgcgcaacgt ccatatctgg
     7021 cggttcgatc tgatccagta tgatccgccg caggcgcgtt tcgaagcccg tgtcggctcg
     7081 ggcacctata tccgctcgat ggcgcacgac ctgggcctcg ccctcggctg cggcgcctac
     7141 ctcagccgcc tgcgccggac ccgcgtcggc gccttcggcg tcgatcaggc catcccgctg
     7201 gccgagctgc gcgccgatcc cgatctgctg ccggcccgcc tgctcggcgt caccgaagcc
     7261 ctgacccacc tgcccaaggt gacgatccag cccgaggccg agcgcctcgt cgccaccggt
     7321 cggagcttca ccaccggcca catcctggaa ttcgagggga tcctcaaccc gggtcagccc
     7381 gtgctggtga tcgataccca gggccgcgcg ctctcgatcg tccgccccga gcccgacgag
     7441 cagttgacgg agacgccggt gctgggcgcc accgccatga ttttccggcc gctgcgggtc
     7501 ctcgcgccgg cctgacgttt cgccccggac ccggtgaaag gcgccgatgg ccgaatcgca
     7561 ccccgccacc gtcccccgcg ccgcgtccgc gcgcccggcc cgctggctgt cgggggcgat
     7621 gctggccctc gcgatcgcgc tgctcgcctg gctcagcctg ggcctggcgc cgcgctggcg
     7681 ggccagcctg gactacccgt ttcaactcga cgccgaggaa ggctttctcc tcaaccaggc
     7741 cgtgcggctg gctcagggac gcggcattta cacgcccatc gatcagccgc cgtatctggt
     7801 cggcaattat ccgccgctct atcccgcgct gtatgccctg gttcacggct cccgcgcgcg
     7861 gccggccagc ctgcccgtgg gccgcgccat cgtccaggcg gcgacggtcc tctcggcgct
     7921 gctgctggcg ctcattgcct ggcgcgcgac gcgtcgtccg gcgcccctgc tgcttgcccc
     7981 ggtccttttc ctggcggcct atgaggtgca tcactggtcg gcgttcgccc gcgtcgatct
     8041 gcccgcgctg gcgcttacgc tggccggcct ggcgctgttt cttgaagggc ggcggccggc
     8101 ccatctggcc gccagcgccc tgttgttcgt cgcggccgcc tgcacccgcc agacggccgt
     8161 gatggcgccg ctggcctgct gcctcctcct ggcgtggacg gaccgccgcg ggctggcctg
     8221 gttcgcgggc gcctgggtcg gcggcggtct gctcgcgctg gccgcgctcc aggcggcgac
     8281 gcgcggcgag ttctggcgcc acgtcgtcgc ctacaacgtc aattcgttcg actggtcggc
     8341 gtggcgcgcg gtcatgcgca acgaagtctg gttcttccac cgctggatcc tgctggcttg
     8401 cgcgggcatt gcgctgggcc cggccgttgc cagcttcagc gcccgccgcc gctccgccgt
     8461 ccccgccgcc aaggcggcga ctgccccacc cccccgcgcg tccgccgcct ggtccgtggc
     8521 gggcctgttc gccctgctca ccaccgcgtt ttccctgccg gcgatggcga aaatcggttc
     8581 cgcccccaac tacctcctcg aaccgctcgc ggcctgggcg ctcgtgattg cgctggcgct
     8641 cggccatgcc ctcgacgggc ggtcaaacgc cggcaccggc ggccgctcag gcgcgcgctc
     8701 ccttgcggcg ctactcctgg cggcatgcct gctcctgcat gcgggccatc tggttcggct
     8761 gcgcccggtc ctccactcca gccgcaatcc gaccccggcg gaccgcgaag ccctccggcc
     8821 gatcgtcgcc gatctgcgcg cccagccggg accggtctac gcagaggagc ccgtcctcgc
     8881 cctgctggcg ggcaaggagg tcctccttca gccgttcatt ctgtcgcagc ttgctcgcga
     8941 aggtcggtgg cgcgacgccg gatgggtcgc catgctcgag gaccggacat tgacccggct
     9001 ggtcgtgaac gaggacctgt cggcggccgg cccggagcag cgttaccgcc gctacaccgc
     9061 cacgacggcg gccgccgtcc gcgaaaacta ccgcccggcc ggcgccatct ccctccccgg
     9121 cctcgccacc tattacgtct gggagccgaa gatggcggag tgaggcgggg gaaattcgta
     9181 tatcgaaatt ctaattagcg aaccgcagct cgtccatcga agcgaaccgt agtcgtccgc
     9241 ccggcaccac gcgtcttatt cgaaatccaa actttgaaat ttggatttct taattgctgc
     9301 gtcgacctcg atcgcgaagg ctgctgcggt tgaattcggt gcgcgacccc agactgctcc
     9361 ggctgcccag ccgatcggta tcccggcggc tgaagtcgct tcggatcgcc gagcgtccgc
     9421 ggtcatcgat caggcttttg cgaatcgaat cgctgcggct gtcggcgcgc cagacgcggt
     9481 cattgttccg gctcagagcc gaactccgcg tccagtcgct ctcgcgccgc tcggtgatcc
     9541 gccgctcggt gcgatcgttc cggtcgatca gccgccgccc cggatcccgg tcgcggtccc
     9601 gatcccaccg cacctgctcg tcgcggcgcc gatcccggtc gctccgatcc caatcgcgat
     9661 gatcgcgatc gcggttccga tcataccgat cccaatcacg ccgatcccag tcgcgatcat
     9721 gatcgtgccg gtcgcggtag atcggccgca cgacgtagtg gttggtgtac gggcggcgga
     9781 cgctgtagta gcgataatcg ctcagatacc gcggccgata gagcgggcgg tcataaatga
     9841 aaaccgtcga cggcgggcag atccacgggc tgacgtagac ctcctcccgg tagtacgtcg
     9901 ggaagccgaa cgaactgtag ctgcggtatt cacgatagac cggcggcgaa tagaataccg
     9961 gccccgagtc atagccgatg cggatgctga aattatcggc cgcagccggc gccggcgccg
    10021 cgatcaccag ggtcaccgcc agcagaaaaa gcattgccca gcgcattgct tggatcctct
    10081 ccgggagcgg ccccgcgggc tcgtccctgt tccacgggct gttgcatgca tgatgcggac
    10141 gaccgccagc ggcgaggccg tcccgccggc ggactgtagt gaaaactata tcacaccctc
    10201 cccgcgccgg ccaacagccg atcgtccgtc cccccccttc tctcgtcctt tctcctcctc
    10261 cccccccccc aaggcgcccc cccccc
//
LOCUS       DAOPCO010000141        10199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6374_length_10199_cov_4.424192, whole genome shotgun sequence.
ACCESSION   DAOPCO010000141 DAOPCO010000000
VERSION     DAOPCO010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10199
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6374_length_10199_cov_4.424192"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLS90_10495"
     CDS             <1..737
                     /locus_tag="PLS90_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK02871.1"
                     /translation="DMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRRPYAVLLF
                     DEIEKAHPDVFHILLQLLDDGRLTDSQGRTVDFKNTVVIMTSNIGSTYLTEGVDEDGE
                     LDENTRLRVMNELRANFRPEFLNRIDDIVLFTPLGLEQIKQIVDLLLEQIYGRLADRR
                     ITLELTDAARAWLANRGYDPTYGARPLKRLIQKELETALGRRLIAGEIRDGSRVQVDV
                     EAGELVFRTDHSAESPVDSAADVATR"
     gene            complement(1101..1379)
                     /locus_tag="PLS90_10500"
     CDS             complement(1101..1379)
                     /locus_tag="PLS90_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02872.1"
                     /translation="MMPIGRTLTGAAIAALAMVGTVAFAESSKVKHDFKYGELRTPTR
                     SLVSVDVDTPEHFEEPFVGPVIVEFEPEGQVFGVVPDMVTGIENESGM"
     gene            complement(1523..1909)
                     /locus_tag="PLS90_10505"
     CDS             complement(1523..1909)
                     /locus_tag="PLS90_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02873.1"
                     /translation="MILASAGLSLWTGLTVCLLVSLICADRNRAAVGAGLILLVVWRV
                     APAFAQAAREIQTSWSQTAEGGAVRAVAEWILDVSPITQLVRTADNSAALSVGRCATF
                     MVVMNLIFSLLLLTPKARKRFDVEYH"
     gene            2155..2679
                     /locus_tag="PLS90_10510"
     CDS             2155..2679
                     /locus_tag="PLS90_10510"
                     /inference="COORDINATES: protein motif:HMM:NF017434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HPK02874.1"
                     /translation="MTQPDPAPADGNGKLLIGWTEYVSLPAWGIQELRAKVDTGARTS
                     ALHVTGLERLAGNRVRFEVILSRKQAHKRVTVETVACKWARVRSSTGIYRRRCFVRTL
                     LRIGPVEKEIELSLVNREKMLFRMLIGRKALEHDFLVDASQRRLLGRGPDGSGSPGAP
                     AGPVSEPPTNRGCP"
     gene            2676..3866
                     /locus_tag="PLS90_10515"
     CDS             2676..3866
                     /locus_tag="PLS90_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HPK02875.1"
                     /translation="MKIAVLSRRLRSYSTRRLKEAAEARGHRCIVLNTLRFSIGLEKE
                     SPELYYKNKPLSSYDAAIPRIGASVTTFGTAVVRQFEQMGIYTANESLAISRSRDKLR
                     TMQILSKHDIGIPPTAFVREKRDVLPAIERIGGAPVIIKVLEGTQGVGVILAETVKVA
                     EAIVETLQSARQNVLLQKFIKESRGRDIRAIVVGDKVVAAMRRMAQEGEFRSNVHRGG
                     TTMALLLDRPYEQAAIRAAQILGLRIAGVDLLESDEGPQVMEVNSSPGLEAIESTTKI
                     DVAGAIVADIEHQVLFPDVDLKQRLRFAGGFGIAEFTVHNMPDLEGKRLRDCGLDRKS
                     IHVIHITRSSSIIPNPRGDDTIERGDTLLCYGALNELRQMMPPQRKSRRRRRAANGTT
                     DAEG"
     gene            complement(3888..5030)
                     /locus_tag="PLS90_10520"
     CDS             complement(3888..5030)
                     /locus_tag="PLS90_10520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK02876.1"
                     /translation="MDRPLRIALANSGRRWIGEVAHVAMLYEQLERLGHEPWLICRAD
                     RPLHEQARERNWRILPLRFEHAFSVGADARDVLAWRRLVRERKIDLIHCHRGKDHWMG
                     WVVARLTGRPLIRTRHVVMPVRRHPLNRWLYLHGTEAVLSVSRAAESGFGAWSEDLPR
                     RRVVLAAVNAERFHPRRRSAEWRRRHVAAPWRAPDAGAPLWFGLIGRYQRVKGHDVFL
                     EAAARVAARVPEAHFLIAGQGPPGQRAKRRRLAGQLGIADRVWIAGHLGNLAKVLASL
                     DVGVIASRGSEGSSRIALEMMAAGLPLAATRVGGIPELVEPCGCARVVPADDPEALAD
                     AMLAWARDPAARQRAGEAGRRRVLARHTPRRWAEQMVQVYREVLLG"
     gene            5598..7292
                     /locus_tag="PLS90_10525"
     CDS             5598..7292
                     /locus_tag="PLS90_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPK02877.1"
                     /translation="MRRQVLINVEATDIRVAMLEDGTLVELFVEDLHTRSKIGNIYKG
                     RVESIVPGLRAVFVNIGCEKNAFLHFNDVLPEFELPQKGRPERERRSQLRGTEAVDAM
                     ADQEEDDDYVDPVVVGETEEAVKNRPRRPLRVGDEILVQVTKEEINNKGPRITTFISL
                     PGRYLVYMPFAERSGGVSRRIEDGDERKRLRDILRDLKPETGSLIIRTAGIDQAEEAI
                     RQDAEQLQKKWQEITRRARRDRSPARVYDDQEIITRVVRDNFTEDIDEILIDSKPAMR
                     ELIRTCQVMVPCLVDRIALYDSPISIFDTFEVEKQFQKALRRKVPLRSGGEIVIDETE
                     ALISIDINSGRYVGHEDQEQTILKINLEACRAIARQLRLRDLGGLIVIDFIDMNEREN
                     ELAVLREFKRCLRSDRAKYAISDFSEFGLVQMTRKRVRKSLAKAIYRPCPYCDGSGRI
                     LTDQQLWKQLKYDLMAELEADPPAAAVRIVVHSEFKTYLQTAMTAELRALAHKYHVAL
                     QIVSAEDYHHERITILKQAKSTHNVGPNNGSGGNGLTQRGAEKKLETAQMARDNAS"
     gene            7815..8798
                     /locus_tag="PLS90_10530"
     CDS             7815..8798
                     /locus_tag="PLS90_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPK02878.1"
                     /translation="MERRIGILGCKHTTLDLIRALRRAGVPLQQVITLPPALGRRHQV
                     AGYLDLRDELDRLAIPWTHATDYALNAEADRRALPRLGLDLLLVMGWQRLVPEWFLDC
                     LPLGAWGMHGSCRPLPHGRGRSPLNWSIITGQDTFYTHLFRYLPGVDDGPVAGQIVFD
                     INPYDTALTLHKKNQIAMIRLVLEKLPAILAGRVEPTPQPAEPPSYWPKRNPEDGIIF
                     WEDPANVICRLVRAVSRPFPGAFCFLDDDPGRKLTIWQAQPFDRRLTWPDAAPGEIVA
                     VFHDATFVVRCGDATVLVTESEGVRLTDEDLGRRLGSAGIKRKVWPNLPPL"
     gene            8881..9348
                     /locus_tag="PLS90_10535"
     CDS             8881..9348
                     /locus_tag="PLS90_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02879.1"
                     /translation="MTRAIGLALIGMLVLLAALEVARYQRAARLEDELPYPRRRLIRR
                     IGVSIIFSLLVAAVVYWPAAAPAAAQLTLMLAVLLGIAFGLFLLWRDLHETSRAVVRH
                     ATRLSEESGSELLRTFESPAAPLRTKDPTRRREEGFSLPPEVDTTGENGKTEG"
     gene            9462..>10199
                     /locus_tag="PLS90_10540"
     CDS             9462..>10199
                     /locus_tag="PLS90_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK02880.1"
                     /translation="MDGELYEKVHKVVIAGSGPAGLTAALYSARAELAPIVVEGFDAG
                     GQLTLTTEVENYPGFEHGITGPELVDVMRRQAERFGTIYLRGDIQRVDLRERPFTIAL
                     EDGRELRTQTFIISTGARARWLNLPAEQALLRAGGGVSACATCDGAFYKGKEVMVVGG
                     GDTALEEAMFLTKFATRVTVIHRRDELRASKIMQQRAFANPKIEFIWNSVVEDVLGVE
                     EKRIRAVVLRNRVSGETTEVPTQGLFIA"
BASE COUNT         1844 a         3718 c         3015 g         1622 t
ORIGIN      
        1 tcgacatgtc ggaatacatg gagaagcact cggtttcgcg cctgatcggc gcgcccccgg
       61 gctatgtcgg ctacgaggag ggcggccagc tcaccgaagc agtgcgccgc cggccgtatg
      121 ccgtgctcct gttcgacgag atcgaaaagg cgcatcccga tgtcttccac atcctgctgc
      181 aactgctcga cgacggccgc ctgaccgatt cccagggacg aaccgtcgac ttcaagaaca
      241 ccgtcgtcat catgacctcc aacatcggct ccacctacct gaccgaagga gtggacgaag
      301 acggcgagct ggacgagaac acgcggctgc gcgtgatgaa cgaactgcgc gccaacttcc
      361 gcccggaatt cctcaaccgg atcgacgaca tcgtgctctt cacgccgctc ggcctggagc
      421 agatcaagca gattgtcgac ctgctgctcg aacagatcta cggccggttg gccgaccggc
      481 gcatcacgct ggagctgacc gatgcggcgc gggcgtggct cgccaaccgc ggctatgacc
      541 cgacctacgg cgcgcggccg ctcaagcgtc tgatccagaa ggaactggag acggccctgg
      601 ggcgccggct gatcgccggc gagatccgcg acggctcgcg ggtccaagtc gatgtcgagg
      661 cgggcgaact ggtctttcgc accgaccact cggctgaatc ccccgtggac tccgcagcgg
      721 atgtcgcgac acgctgatcg gccatcctgg agtgcggtga cagagcgttg caacgcaacg
      781 cgccgatacc gcttcgggtc gacgactcgc tggacgcatc cccggtctgg atgcgtttcc
      841 gtcttggcgt cgttgcctgt tgcgcctatg tctttgtgcc ttgctttgct cagaatgatc
      901 gctgacgctc acatcgcgca ggtctgtttc ggttcttgaa attgcacttt ccaatttgtt
      961 tcgcatttcg atatacgatt ggcgtgcttc ttcccgcttc aagcgaactt cttcccgctc
     1021 caagcgaaaa ggggcggcgg aaaaatccgc cgcccccgga tccggaacgt gatccgcctc
     1081 tgcggttgct tctccggtga tcacatccca gactcgttct caatcccggt caccatgtcc
     1141 ggaacgacgc caaacacctg accttccggc tcaaactcca caatcaccgg cccgacaaac
     1201 ggttcttcaa agtgttccgg ggtatcgaca tcgaccgaca ccagactccg cgtcggcgtg
     1261 cgcagctccc cgtatttaaa gtcatgcttg accttgctgc tctccgcgaa cgctaccgtc
     1321 ccaaccatcg ccagggcggc gatggcggct cccgtcaagg tccgtccgat gggcatcata
     1381 ctccaaccct cctctctccc cgcgcggggg attgaatgct gcgtgtgcgg tttcacctct
     1441 cctggtggca cacggcaggg tttaaagcac aacgcgggcc aatcccctcg gctggggacc
     1501 gcccggcaac tcccattatg acttaatgat actcgacgtc aaaccgttta cgcgccttgg
     1561 gcgtcagcaa caggagtgaa aagatcagat tcatcacaac cataaaggtt gcgcaccgcc
     1621 cgacgctcag cgccgcggaa ttgtccgcgg tgcggacgag ttgcgttatc ggcgacacgt
     1681 ccagaatcca ttcggcaacc gcccgcacgg cgcccccctc cgccgtctga ctccagctcg
     1741 tctggatttc gcgcgcggcc tgcgcaaacg ccggcgccac ccgccagacc accagcagaa
     1801 tcagccccgc ccccaccgcc gcccggtttc ggtcagcgca gatcagactt accagcaggc
     1861 aaaccgtcag gcccgtccac agcgacaaac ccgccgaggc aagaatcatc cgagcgttat
     1921 ccccgatcct cgtccctcgc ccctcgtccc tcgtcttcgt attcgcctcc aaaggcccgc
     1981 cgaaattccg ttcccggttc ttccccctca accccgaatc cgacaccagc gttgatgtcc
     2041 ccgccgccgc cgtgatatgc agaaggtgga caaccgctcg cttccatccc tcctgatgac
     2101 gcacgccggc gcgggaagtt gcaccgcccc atcctccggg caaggaaacc tttcgtgaca
     2161 caaccggacc cagcccccgc cgacggcaac ggcaaactgc tgatcggatg gaccgagtac
     2221 gtctcattgc ccgcgtgggg cattcaggaa ctcagggcca aggtcgatac gggcgcgcgc
     2281 acctccgcgc tccacgtcac cggcctggag cggctggcgg gcaaccgcgt gcgcttcgag
     2341 gtgatcctga gccgcaaaca ggcccacaag cgcgtcacgg tcgaaacggt cgcctgcaaa
     2401 tgggcccggg tccgctccag caccggcatc taccggcgcc gctgcttcgt gcggacgctg
     2461 ctgcggatcg gccccgtcga aaaagaaatc gagctttccc tggtcaaccg cgagaaaatg
     2521 ctgttccgca tgctgatcgg tcgcaaagcc ctggagcatg acttcctcgt ggacgcctcg
     2581 cagcgccggc tgctcggccg cggcccggat gggtcgggct cccccggcgc gccggccggc
     2641 cccgtctccg agccccccac gaaccgaggt tgcccgtgaa aatcgccgtc ctctcacgca
     2701 ggctccgttc ctacagcacg cgccggctca aggaagcggc cgaggcgcgc ggccaccgct
     2761 gcatcgtcct caataccctg cgcttctcga tcggcctgga gaaggaatcg cccgaactct
     2821 attacaagaa caagcccctc tcgtcctacg acgccgccat cccgcgcatc ggcgcctcgg
     2881 tcaccacatt cgggaccgcc gtcgtccgcc agttcgagca gatggggatc tacaccgcca
     2941 acgaatcgct ggcgatctcg cgctcgcgcg acaagctgcg cacgatgcag atcctgagca
     3001 agcacgatat cggcatcccg ccgacggcgt tcgtgcgcga aaagcgcgac gtcctgccgg
     3061 ccatcgaacg gatcggcggc gcgccggtca tcatcaaggt gctggaggga acgcagggcg
     3121 tcggcgtcat cctggccgag acggtcaaag tggccgaggc gatcgtcgag accctgcaaa
     3181 gcgcccgcca gaacgtcctg ctgcagaagt tcatcaagga gagccgcggg cgcgacatcc
     3241 gcgccatcgt cgtcggcgac aaggtcgtcg ccgccatgcg ccgcatggcc caggaggggg
     3301 agttccgcag caacgtccat cgcggcggga cgacaatggc cctcctgctc gaccggccct
     3361 acgaacaggc ggccatccgc gccgcccaga tcctcggcct gcgcatcgcc ggggtcgatc
     3421 tgctcgaatc ggacgaagga ccccaggtga tggaggtgaa cagctcgccc ggcctcgaag
     3481 cgatcgaatc gaccaccaag atcgacgtcg ccggcgccat cgtcgccgat atcgagcatc
     3541 aggtcctctt ccccgacgtc gacttgaaac agcgcctgcg cttcgccggc ggcttcggca
     3601 tcgccgagtt caccgtccac aacatgcccg acctcgaagg caagcgcctg cgcgactgcg
     3661 gactggacag gaaaagcatc cacgtcatcc acataacgcg ttccagctca atcattccca
     3721 atccgcgcgg agacgatacg atcgaacgcg gcgacaccct gctgtgctac ggcgccctca
     3781 acgaattgcg ccagatgatg cccccccagc gcaaatccag acgccgccgc cgcgccgcca
     3841 acgggacgac cgacgccgag ggctgacccc caccccggcc cgccccgcta ccccagcagc
     3901 acctcgcggt aaacctggac catttgttcg gcccaccggc ggggagtatg gcgcgccagc
     3961 acgcgccgcc gcccggcctc ccccgcgcgc tgccgcgccg ccggatcgcg cgcccaggcc
     4021 agcatggcgt cggccagcgc ctccgggtcg tcggccggca cgacgcgcgc gcagccgcac
     4081 ggctccacca gttccggaat cccccccacg cgcgtcgccg ccagcggcag gccggccgcc
     4141 atcatctcca gcgcgatccg gctcgaaccc tccgagccgc gcgaggcgat cacgccgaca
     4201 tcgagcgagg ccagcacctt ggccagattg cctagatgcc ccgcgatcca cacccggtcg
     4261 gcgatgccga gctggcccgc cagccggcgg cgcttggcgc gctgtccggg cggaccctgg
     4321 cctgcaatca gaaaatgcgc ctcgggaacc cgcgccgcca cgcgcgcggc cgcctccaga
     4381 aacacatcgt gccccttgac ccgctggtag cgaccgatca ggccgaacca gagcggcgcg
     4441 ccggcgtccg gcgcgcgcca cggcgcggcc acgtgacgcc ggcgccattc cgcgctccgc
     4501 cgccggggat gaaaccgctc ggcattgacc gccgccagca cgacccgccg ccgcggcaga
     4561 tcttcgctcc acgcgccaaa cccgctttcg gccgcgcgac tgacgctcag cacggcctcc
     4621 gtcccgtgca gatacagcca gcggttcagc ggatgacgcc gcaccggcat caccacgtgg
     4681 cgcgtccgga tcagcggccg gcccgtcagt cgcgccacca cccaccccat ccagtggtcc
     4741 ttgccgcggt ggcagtggat cagatcgatc ttgcgctcgc gcaccagccg ccgccaggcc
     4801 agcacgtcgc gcgcatccgc ccccacggaa aaggcatgct cgaaccgcag cggcaggatg
     4861 cgccagttgc gctcgcgcgc ctgctcatgc agtggccggt ccgcgcgaca gattagccac
     4921 ggctcgtgcc ccagccgctc gagctgttca tacagcatgg cgacatgggc cacttcgccg
     4981 atccagcgac gtccgctatt ggccagggcg atgcgcaacg ggcggtccat accgggcctc
     5041 accatagccg cgggggcgac cgccatcaat gccaatcggc cccgctccgg ccggccgcgc
     5101 gccccgcccc acctcgaact ccgtccgacc ggccgggtcg gaccgatttt aagttcgaca
     5161 ccgcaacaat ttcattgcgc cgaccgcccg atccgttgca ggatcgtatg ggcctactgg
     5221 acgcccgggg aacctttggc gagcgcccgc gtcagccagc caaacagcgg cgggccgtta
     5281 cgaacaggtt ctccccggcc ggcagcgccg ccgattttcg gtggcgcgcc cgggcgcctt
     5341 ccccttttgg gtcggctccc catgcgaacg ggttttattg ggactgagtg ttcgaaagtc
     5401 aatccgagaa ccggttgttg aaccgcgtcg gacgcggcgg ccttggctgt cacctctccg
     5461 caccgcgcgg ccagcatgcg ggcacgcttg tgtccgactg agccgccgct atcggtctcc
     5521 cggcgcttcg cgccctcacc atcccctccc ccaacccgac gcacaccctt ccttccaagg
     5581 agcatcgacg cccatccatg cgccgacaag tcctgattaa tgtggaggcc accgacattc
     5641 gggtggccat gctggaagac ggcacgctgg ttgaactttt tgtcgaggat ctgcacaccc
     5701 gttccaagat cggcaacatc tacaaggggc gcgtcgaaag catcgtcccc gggctgcgcg
     5761 cggtcttcgt caatatcggc tgcgagaaaa acgccttcct gcatttcaac gacgtcctgc
     5821 ccgagttcga gctcccccag aagggccgtc ccgaacgcga gcgccggtcc caactgcgcg
     5881 gtaccgaagc ggtcgacgcg atggccgacc aggaggagga cgacgactat gtcgatccgg
     5941 tcgtcgtcgg cgagaccgag gaggccgtca agaaccggcc gcgccgcccc ctccgcgtcg
     6001 gcgatgaaat cctcgtccag gtcaccaaag aggagatcaa caacaagggc ccgcgcatca
     6061 ccaccttcat ctcgctgccc gggcgctatc tcgtctacat gccgttcgcc gagcgctccg
     6121 gcggcgtctc gcgccgcatc gaggacggcg acgagcgcaa acgcctgcgc gacatcctgc
     6181 gcgacctcaa acccgagacc ggctccctca tcatccgcac ggccggcatc gatcaggccg
     6241 aggaggccat ccgccaggac gccgagcagc tgcagaaaaa atggcaggaa atcacccgcc
     6301 gcgcccgccg cgatcgctcc cccgcccgcg tctacgatga tcaggagatc atcacccgcg
     6361 tcgtccgcga caacttcacc gaagacatcg acgagatcct gatcgactcc aagccggcca
     6421 tgcgcgagct gatccgcacc tgccaggtga tggtcccctg cctggtcgat cgcatcgccc
     6481 tctacgattc accgatcagc atcttcgata ccttcgaggt ggagaagcag tttcagaaag
     6541 cgctgcggcg caaggtcccg ctgcgcagcg gcggcgagat cgtcatcgac gaaaccgagg
     6601 ccctcatctc gatcgatatc aacagcgggc gctatgtcgg ccacgaggat caggaacaga
     6661 ccatcctgaa gatcaacctg gaggcgtgcc gggccatcgc gcgccagctc cgccttcgcg
     6721 atctgggcgg cctgatcgtc atcgatttca tcgacatgaa cgagcgcgag aacgaactgg
     6781 cggtcctccg cgaattcaag cggtgcctcc gctccgatcg cgcaaagtat gcaatttcag
     6841 acttctctga gttcggactg gtccagatga cgcgcaagcg cgtccgtaag agcctcgcca
     6901 aggcgatcta ccgcccctgc ccctattgcg acggcagcgg ccggatcctg accgaccagc
     6961 aactgtggaa acagctcaag tatgacctga tggcggaact cgaagccgat ccgccggccg
     7021 ccgccgtccg catcgtggtg cattctgaat tcaagactta ccttcaaacg gccatgaccg
     7081 ccgagctgcg cgccctggcc cacaagtatc acgtcgcgct ccagatcgtc agcgccgaag
     7141 actaccacca cgagcggatc acaattctaa agcaagcaaa atcaacgcat aacgtggggc
     7201 ccaacaacgg ctcaggagga aatggactca cgcagcgggg ggcggaaaaa aaacttgaaa
     7261 ccgcccagat ggcccgtgat aatgcctcct agagtcaact ccgaccaggt tgacttctct
     7321 caagggactt cttcccctcc tctcttttga agaagtctcc tttcctgcag tcccttccga
     7381 aggccatgcc gacggaaggt gttgcaacca ccccactacc ccttgcggga cgggctgtct
     7441 gcgggcccgt cccgcaaatt ttttccacgc ctccccagca tgaatctggc tcgatctcgt
     7501 cctgagatga agcgccgcaa gaatacgcat cccccggaga aggaaccctt gcgtcgggtg
     7561 cgcaaaccga ccgctccgcc cggacaggcg caccgcgatc gcggccggga agccagccgc
     7621 cgggcgtgcc gcgcgctgcc gccgtccgac gccgaggaac tcgccgaccg ccctccaggt
     7681 tccgacggag cctgagtgcg tcacgtccct cgtccctcgt cctcgccccc cagcgtctac
     7741 cccgaatagc agttgacgac cctcgcaacg ggcttgatca tgcccatgag tccgcggaaa
     7801 ccacgaggcg cgcgatggag cgacgaatcg gcattctggg ctgcaaacac acgacgctgg
     7861 acctgatccg cgccctgcgg cgcgccggcg tgccgctcca gcaggtcatc accctccccc
     7921 cggcgctcgg ccggcggcac caggtggccg gctatctcga cctgcgcgac gaactcgatc
     7981 ggctggcgat cccctggacg cacgcgaccg attacgctct caacgccgag gccgaccgcc
     8041 gggcgctgcc gcggctcggc ctcgacctgc tgctcgtcat gggctggcag cggctcgtcc
     8101 ccgaatggtt cctcgattgc ctgccgctcg gcgcctgggg catgcacggc tcgtgccggc
     8161 cgttgcccca tggccggggg cgcagtccgc tcaactggtc gatcatcacc ggccaggata
     8221 cgttctacac ccacctcttc cgctatctgc cgggggtcga cgacggcccc gtcgcgggtc
     8281 agatcgtctt cgacatcaac ccctacgaca ccgcgctgac gttgcacaag aagaaccaga
     8341 tcgcgatgat ccgcctggtt ctggagaagc tgcccgcgat cctggcgggt cgcgttgagc
     8401 cgaccccgca gcccgccgag ccgccatcat actggcccaa acgcaatccc gaggatggca
     8461 tcatcttctg ggaggacccg gccaacgtga tctgccgcct cgtgcgcgcc gtgagccgcc
     8521 cctttcccgg ggcattctgt ttcctcgacg acgatcccgg ccggaaattg acgatctggc
     8581 aggcgcagcc gttcgaccgt cgcctgacct ggcccgacgc cgcgccgggc gagatcgtcg
     8641 cggtgttcca cgacgcgaca ttcgtcgtcc gctgcggcga cgccacggtc ctcgtgaccg
     8701 aaagcgaggg cgtccggctc acggacgagg atctcgggcg gcggctgggt tcagccggaa
     8761 tcaagcgcaa ggtctggccc aacctgcccc cgctctgaga ccggccgacg gccggccgac
     8821 ctcaattccc aacgagattg atgctatgcc ccttctctcc aattcacggc ggtgttctcc
     8881 atgacacggg cgatcggcct ggcgctcatc ggcatgctcg ttctgctggc ggcgctggaa
     8941 gtggcccgct accagcgcgc cgcgcgcctg gaggatgaac tcccctaccc ccgccgccgg
     9001 ctgatccgcc gcatcggcgt ctcgatcatc ttcagtctgc tggtggcggc ggtggtttac
     9061 tggccggcgg cggcccccgc cgcggcgcaa ctgactctga tgctcgccgt gctgctcggc
     9121 atcgccttcg gcctgttcct gctctggcgc gacctgcacg agacctcccg cgccgtggtc
     9181 cgccacgcca cccgactttc cgaggagtcg ggaagtgaac tcctccgcac cttcgaatca
     9241 cccgctgcgc cgcttcggac gaaagaccca acacggcggc gtgaggaagg gttcagcctg
     9301 ccgcccgagg ttgacacaac cggcgaaaac ggaaagactg agggctgagt acgccgcatc
     9361 ctcatcgagt gtgagatccg gcgtgcgggg ccgggatgtg ccgacgggaa cgaattcccg
     9421 gcatggcttc cccggcaaac cagataccag aacgggctga aatggacgga gagctctacg
     9481 aaaaagttca caaggtggtg attgccggat cgggtccggc cggcctgacg gcggccctct
     9541 attcagcccg ggccgagctg gcgccgatcg tcgtcgaagg ctttgacgcc ggcggccagc
     9601 tcaccctcac gaccgaggtg gaaaattacc ccggcttcga gcacggcatc accggtcccg
     9661 aactggtcga cgtgatgcgc cgccaggccg agcgcttcgg cacgatctac ctgcgcggcg
     9721 atatccagcg cgtcgacctg agggagcgcc ccttcaccat cgcgctggaa gatggacgcg
     9781 agttgcgcac ccagaccttc atcatctcga ccggcgcccg cgcccgctgg ctgaacctgc
     9841 ccgccgaaca ggcgctgctg cgcgcggggg gcggtgtctc ggcctgcgcc acctgcgacg
     9901 gcgccttcta caagggcaag gaggtgatgg tcgtcggcgg cggcgacacc gctctggaag
     9961 aggcgatgtt cctgacgaaa ttcgcgacca gggtcaccgt catccaccgg cgcgatgagc
    10021 tgcgcgccag caaaatcatg caacagcgtg ccttcgccaa ccccaagatc gagttcatct
    10081 ggaattcggt ggtcgaggac gtgctgggcg tcgaggaaaa acggatccgc gccgtcgtgc
    10141 tgcgcaaccg cgtcagcggc gaaacgaccg aagtgccgac ccagggcctg ttcatcgcc
//
LOCUS       DAOPCO010000142        10150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6404_length_10150_cov_4.614364, whole genome shotgun sequence.
ACCESSION   DAOPCO010000142 DAOPCO010000000
VERSION     DAOPCO010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10150
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6404_length_10150_cov_4.614364"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLS90_10545"
     CDS             <1..341
                     /locus_tag="PLS90_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02881.1"
                     /translation="SRNTLEGGRGYGIADPAQLAEAGVPAEAIELVQQLFPLGVQSHR
                     FTIIARGTYQGVRETIAATVNLTVESYPYDPDDPDTFGLRDQAAAGELENQPYVKVDP
                     AVRVEWMAEL"
     gene            complement(405..>1805)
                     /locus_tag="PLS90_10550"
     CDS             complement(405..>1805)
                     /locus_tag="PLS90_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02882.1"
                     /translation="ANFHENDVLMKSWNQIVARRGANGNGRLRKRERDESLHEGVQRI
                     SVLFRDRTFSDWVIHANPSVKKLLDRTIAKVDSDINQQNVHYGWGHFEELESMTFTPR
                     AIINFQQKLVKLTELGYLPLSPDFYIRGKLRGEFGCTHNEPQVLEMPPASVGNGWNLS
                     NATCFGRWDGLRQNPETGAWELDARPYERPGDNGARETMKGNPCWKIAWNRAREACYR
                     AVVGDLAQLKGGMAEVLAGLSGQTDPDKARRNVEDFLANYTYVYWREFFIQHDMAEAD
                     INIIEIVNKHLRAGVKGELAEADCARAGAAAQAIFFALESGRSMGSGWENMDQRSFYQ
                     NVVMLTLAICNAMYVHHWMGQKAQARKLLSLLQTELLDFENAYTRHNLAEFGVSQKDW
                     KEAIRSEVSECRDNVVRRAALRIAARHLRPLGYTREFTRQDANITTNVGHMWSIESCR
                     ENFCYENTAFCGVLEA"
     assembly_gap    1806..1815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1816..2551)
                     /locus_tag="PLS90_10555"
     CDS             complement(<1816..2551)
                     /locus_tag="PLS90_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02883.1"
                     /translation="MSDQSTINLFHPIYHSFTYPIGCRIDVERILPADIDGEDDEELV
                     DNFSEPPVWIERFSPLAVQIGDQVLRGKNAPDLMLKLYRLMFDLFQGIGRFIDSTINQ
                     SLGHSGRFPFMSLTIDPDTIHRIIEADFEQGETTYPALLRLFGEGYISPCLTTPFHVL
                     LPRLASDSEIRVCIRSAFIFYLRIIRIYTDFLEANGESGLTVVPFWLPEGAVSSRVVD
                     ILNEEFEAFCKREKLRNGHLVLLLDNH"
     gene            complement(3170..3850)
                     /gene="pyrE"
                     /locus_tag="PLS90_10560"
     CDS             complement(3170..3850)
                     /gene="pyrE"
                     /locus_tag="PLS90_10560"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPK02884.1"
                     /translation="MEPYKQQFIDFMLRSGALKFGDFVTKSGRNTPFFINLGLFNSGS
                     RIAQLGDYYALALRDRFGDDFDCLFGPAYKAIPLVVAASIALARHHERDVPFCFNRKE
                     AKDHGEGGNLVGHIPAAGQRVVIVEDVTTAGTSVRETRDLLAPLAGVRLVGLLVAVDR
                     QERGPTGKQSALAALRDDLGLATHAIVTLDEILAHLQGRRVGGKLILTPALLSRIEAY
                     RRQYGAAE"
     gene            4055..5050
                     /locus_tag="PLS90_10565"
     CDS             4055..5050
                     /locus_tag="PLS90_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPK02885.1"
                     /translation="MSRKLVVLNPDHCMEVQFDQQITIGRDVFNSLSLQDPEVSRSHA
                     IIFEQDGEVLIKDLRSRNGIFVRGEKTTESVLRDGDEIILGSTVLIYNPSDTLDPASV
                     LSKRGRYLIEKQSASKSARRAAPATIFSAREMEDAVATLFSKGPDSTEGTFFTHENAI
                     FLLRLMHEMNGAPEAGALCERTLTLAVQFLGGHHGVIMETDEPKQHLKVRALFSKEES
                     DSIRVAQPILKILLGSEKSLFCPDVAHDRRFENLSGQTERPIHSFVATPIRSGDVLFG
                     IIYIDSEDRSVTYDFAALRTLYLIADQLGALMRNRPLHFPKHPVARQQIAPALGS"
     gene            5101..5838
                     /locus_tag="PLS90_10570"
     CDS             5101..5838
                     /locus_tag="PLS90_10570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPK02886.1"
                     /translation="MLVILTGHGAALVQWGRTPPLRSGTVRRAALTWGCAAIGLVTAL
                     VVLRWSLEPALSLWCVQAVGLALVIRMGAYWAARTGVEPFGLFGANWRAWPKRSLALR
                     LAAMLLWTALMLPFAGAGGSVGYARQLGLDALSPWRIGAYVLGVLTLAPILEEGFFRH
                     YLLHGLTAWLGPGAWGTAGAAVVTSLAWSVGHLGLVEPWGLRLLHVFGLGLLLADTSR
                     RHGLDAVIALHWLFNLALIPLLFALGD"
     gene            5862..6818
                     /locus_tag="PLS90_10575"
     CDS             5862..6818
                     /locus_tag="PLS90_10575"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK02887.1"
                     /translation="MTWPDAPLLLALDAPPAPGRPEEEVLQPGSPEARVIVGVEDEAA
                     ELGRADILLTWRRAVDAALLERAARCRLVLHYGPGAGAGVGRVDRDAARRLGIYVASI
                     PDYAAEEWAEGMWELNAALEARRPPAPPFRRRPRVGLIGLGQVGRRLAERWKRAAGEI
                     WAHDPYVDDDVFERAGARRAELTDVFGICDQVLIQLSPAPGATGLIGRDMLELMRPWR
                     QVVCGSRPEVFVETELRWMLERGRPGGLGVLEPMLAGAAAWDWLRARERVVVHAFGEE
                     LNRRMWRSGRRRAAEVVLTMLRGGRPGHLLIDPPCPRHEMWG"
     gene            complement(6897..8807)
                     /locus_tag="PLS90_10580"
     CDS             complement(6897..8807)
                     /locus_tag="PLS90_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPK02888.1"
                     /translation="MNIISVNNLAKSHGAKPLFADLSLGVDERDRIGIIGPNGSGKTT
                     LLRILARLEPPDAGQVAWRQDLRIGLMPQDPRFPPRRTILDCVFTDSPLSQLIHDYEL
                     ACRRLAEHPGDAAIQRQVDRLGRRMEAEAAWERDTAARTILTRLGLDAWERPAAELSG
                     GERRRIALAAALLGEPELLILDEPTNHLDADTVQWLEEELDAFPGAILLVTHDRYFLD
                     RVTNRIWEIANRRIFVHEGHFSDYLANRARREEAEARLEDHRRTILRKELAWLRRGAR
                     ARTTKQKARIDRIETLRERAPDASPDELTFAVRGRRLGKKIVELREIQAGYHDHVLIR
                     NFSYTFTRGERLGLIGPNGSGKSTLLNVLTGRAAPQAGQVIVGPTVHFGYYDQETADL
                     DPSEKTLDYVLREGGHMLRQPDGAVLTAPVMLERFGITPQMQHAPIAKLSGGERRRLY
                     LVRTLLRDPNFLILDEPTNDLDLPTLNALEDFLDGFAGCLLVVSHDRFFLDRTIDRVL
                     SLEPDGVPRLWPGDYSTYRALREEEEQARRREARAEAKADRPPPARRPAAPSARLTYK
                     EQRELERLEEEIPRMEARLAALHQAMAEAATDYERLRKLSEEQAVLQTAIDDAMTRWE
                     HLSERQQSPSIT"
     gene            complement(8804..9943)
                     /locus_tag="PLS90_10585"
     CDS             complement(8804..9943)
                     /locus_tag="PLS90_10585"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPK02889.1"
                     /translation="MPRATDISPGSQSDRRPHRPWSVYLLTVFGIPIYVHITWLLLIG
                     FAAYVEIFRGATLLGGVLFLLAVFASIVLHELGHALVARRFGIRTDDIVLYPIGGVAR
                     LRSMGEGLQELWIALAGPAVNLLIAFALGLVLLATGGFVPVTDDSRLAGIPFAQQLLL
                     INLLLMLFNLIPAFPMDGGRVLRSLLTLATSREKATAIAAKIGQVLAIGFLLLAPFNP
                     ILLFIGVFIFFAAGQEYAATQSVSMMSGHHVEDAMIRHFEVLHHGDSLGRAADLLLST
                     SQHDFPVMSGSDVIGLLTRNELIQGLALHGRDHYVAHVMTRDFPRTTPGEPLQTAFER
                     MRDAGNLPLLVFQNDRLVGYINAENLMEYLMVRQAGRAQESRAGA"
BASE COUNT         1633 a         2731 c         4038 g         1738 t
ORIGIN      
        1 ggtcgcggaa tacgctggaa ggcgggaggg ggtatggcat tgccgatccg gcgcagctcg
       61 ccgaagcggg ggtgccggcc gaagcgatcg agctggtgca gcaactgttt ccgctggggg
      121 tgcaatcgca ccggttcacg atcatcgcgc gggggacgta tcagggggtg cgcgagacga
      181 tcgcggcgac ggtgaacctg acggtggaat cgtatccgta tgatccggac gaccccgata
      241 cattcgggct gcgcgaccag gcggcggcgg gcgaactgga aaatcagccg tacgtcaaag
      301 tggatccggc ggtgcgcgtg gaatggatgg ccgagttgtg acgggatcgt cgcgcgggtg
      361 gcgggcgtgg gggcccggac gccgacgaga cgggttccga tgcgtcaggc ttcgaggacg
      421 ccgcagaagg cggtgttttc gtagcagaag ttttcgcggc aggactcgat cgaccacatg
      481 tggccgacat tggtggtgat gttggcgtcc tgacgggtga attcgcgggt atagccgagg
      541 gggcgcagat ggcgcgcggc gatgcggagc gcggcgcggc ggacgacatt gtccctgcac
      601 tcgctcacct cggagcggat ggcttccttc cagtccttct gcgagacgcc gaactcggcc
      661 aggttatggc gggtgtaggc gttttcgaaa tcgagcagtt cggtctggag cagggagagg
      721 agtttgcggg cctgggcttt ctgccccatc cagtgatgaa cgtacatcgc gttgcagatg
      781 gcgagggtca gcatgacgac gttctggtag aacgagcgct gatccatgtt ttcccagccg
      841 gagcccatgg agcgcccgct ttcgagggcg aagaagatgg cctgggcggc ggcgccggcg
      901 cgcgcgcaat cggcctcggc gagctcgccc ttgacgccgg cgcgcaggtg cttgttgacg
      961 atctcgatga tattgatgtc ggcctcggcc atatcgtgct gaatgaagaa ctcgcgccag
     1021 tagacgtagg tgtaattggc gaggaagtcc tcgacgttac ggcgggcctt gtcggggtcg
     1081 gtctggccgg agaggccggc gaggacctcg gccatgcccc ccttgagctg ggcgagatcg
     1141 ccgacgacgg cgcgatagca ggcctcgcgg gcgcggttcc aggcgatctt ccaacagggg
     1201 ttgcccttca tggtttcgcg ggcgccgttg tcgccggggc gctcgtaggg gcgcgcgtcg
     1261 agttcccagg cgccggtctc cgggttctgg cggaggccat cccagcggcc gaaacaggtg
     1321 gcgttgctga ggttccagcc gttgccgacg ctggcggggg gcatctcgag gacctgcggt
     1381 tcgttgtgcg tgcagccgaa ctcgccgcgc agcttgccgc gaatgtagaa gtcgggggag
     1441 agggggaggt agcccagttc ggtgagtttg acgagtttct gctggaagtt gatgatcgcg
     1501 cgcggggtga aggtcatcga ttcgagttct tcgaaatggc cccagccgta gtggacgttc
     1561 tgctgattga tgtcggagtc gaccttggcg atggtgcgat cgaggagctt cttgacggag
     1621 gggttggcgt ggatgaccca gtcgctgaag gtgcggtcgc ggaagaggac gctgatgcgc
     1681 tggacgcctt cgtggagcga ctcatcgcgc tcgcgcttgc ggaggcggcc gttgccgttg
     1741 gcgccgcggc gcgcaacgat ctggttccat gacttcatca ggacgtcgtt ctcgtggaaa
     1801 ttggcnnnnn nnnnngatgg ttgtcgagca ggaggacgag atggccgttg cgaagcttct
     1861 cgcgcttgca gaacgcttcg aattcttcat tgaggatgtc gacgacgcgg ctggagacgg
     1921 cgccctcggg gagccagaac gggacgacgg tcaggccgct ttcgccgttg gcctcgagga
     1981 agtcggtgta gatgcggatg atgcgcagat agaagataaa ggccgagcgg atgcagacgc
     2041 ggatttcgga atcggacgcg aggcggggga ggaggacgtg aaacggcgtc gtgaggcagg
     2101 gtgagatgta gccctcgccg aagagccgca ggagagcggg gtaggtggtc tcgccctgtt
     2161 cgaagtcggc ctcgatgatc cgatgaatgg tgtcgggatc gatggtgagg gacatgaagg
     2221 ggaagcggcc gctgtggccg aggctctggt tgatggtgga atcgatgaag cggccgatgc
     2281 cctggaagag gtcgaacatc aggcggtaga gcttgagcat gaggtcgggg gcattcttgc
     2341 cgcgcagaac ctggtcgccg atctggaccg cgaggggaga gaaccgctcg atccagacgg
     2401 ggggttcgga gaaattatcg accagttcct catcatcctc gccgtcgatg tccgcgggga
     2461 ggatgcgctc cacgtcgatc cggcagccga tggggtaagt aaagctgtga tagatgggat
     2521 ggaagagatt gatggtggac tgatcggaca actggccatc gttgacgccg atggactgac
     2581 ggaaaccggg acgtgtagct tttgcgggac tcaaaggaac cctcctcaga cctctacagc
     2641 ggcggtttgg cggacgcagg gaatgcgccg gatgggaacc acccggccgg acccctccgg
     2701 tcggccggat tgcgttcggg cgacaaagtc gacggctgtc agtgttgagc ggcccgcgac
     2761 cctccaactt cccgtcggaa agggtggtat ctggacagag ttgcgatgcg gtaacccacg
     2821 ccacgcctct aaagcgttta ccgttaggta ttagaccata cggcgcggcg cggcgtcaat
     2881 gggaatctgc gtcatgggcg gaaaaccgtg ggaaagcgcg tattttcggc gccgggcgga
     2941 acggacggcg agagaactga gcgtcgaatc gggcgcgcag ggctctgatt tccggaactg
     3001 cgagtcatca tttgctggac ctgcgttgtt gggcgcagcc ccgttgcggg ccgccaccgc
     3061 gcgggcgcgg ggaagttgga aatattaatg cgtggaatgc taatggctcg agtttcggcg
     3121 gagattgcat tagccattgg gatcaggcgg gcgggaccgg agggcggcgt cactccgcgg
     3181 cgccgtattg ccggcgatag gcttcgatcc gcgagagcag cgcgggggtg aggatcagct
     3241 tgccgccgac gcggcggccc tgcaggtggg cgaggatttc gtcgagggtc acgatggcgt
     3301 gggtggccag gccgaggtcg tcgcgcaggg cggccagggc ggactgcttg ccggtcgggc
     3361 cgcgctcctg gcggtcgacg gcgacgagca ggccgaccag ccggacgccg gcgagcgggg
     3421 cgagcaggtc gcgggtttcg cggaccgaag tgccggcggt ggtgacgtcc tcgacgatca
     3481 cgacgcgctg gccggcggcc gggatgtggc cgacgaggtt gccgccttcg ccgtgatcct
     3541 tggcttcctt gcgattgaaa cagaatggga cgtcgcgctc atgatggcgg gcaagggcga
     3601 tcgaggcggc gacaacgagc gggattgcct tgtaggccgg accgaagagg cagtcgaagt
     3661 cgtcgccgaa gcggtcgcgg agcgcgaggg cgtaatagtc gcccaactgg gcgatgcggg
     3721 agccgctgtt gaaaaggccg agattgatga agaagggggt gttgcggccg ctcttggtga
     3781 cgaagtcgcc aaacttgaga gcgccgctgc gcagcatgaa atcgatgaac tgctgtttgt
     3841 agggttccac ggacgcgccc tcccgcgcgg ggcgtaccac gatgacgcga aagccccgtt
     3901 gaatcaaggc gaaagccgcg cgcggggggc aagaattcat tggcgggagg ggccggcgga
     3961 cctatacttt ggggaaggca cggcccgcgt cgtccagcgg gggcgtgcgc caatcccagc
     4021 ccctcgcccc tcgtccgacg cggaggtatg ctcgatgagc cgcaagctgg tggtcctcaa
     4081 tccggaccac tgcatggaag tccagttcga ccagcagatc acgatcgggc gcgatgtgtt
     4141 caattcgctg agcctgcagg atccggaggt atcgcgctcg cacgcgatca tcttcgagca
     4201 ggacggggaa gtgctgatca aggatctgcg cagccgcaac gggattttcg tgcgggggga
     4261 gaagacaacc gagagcgtgc tgcgcgacgg cgacgagatc attctgggct cgacggtgct
     4321 gatctacaat ccgtcggaca cactggaccc ggcgtcggtt ctgtcaaagc gcgggcggta
     4381 tctgatcgag aagcagtcgg cctcgaagag cgcgcggcgg gcggcgccgg cgacgatctt
     4441 ctcggcgcgc gagatggagg atgcggtcgc gacactgttc agcaaggggc ccgattcgac
     4501 cgaggggacg ttcttcacac atgagaacgc gatcttcctg ctgcggctga tgcacgagat
     4561 gaacggcgcg ccggaagcgg gggcgctgtg cgagcgaaca ctgacgctgg cggtgcagtt
     4621 cctcggcggg catcacggcg tgatcatgga gacggacgaa ccgaagcagc atctgaaggt
     4681 gcgggcgctg ttctcgaagg aagagagcga ttcgatccgc gtggcgcagc cgatcctgaa
     4741 aatcctgctg ggttcggaga aaagcctgtt ctgtccggac gtcgcgcatg accggcggtt
     4801 tgagaatctg tcggggcaga cggaacggcc gatccattcg ttcgtggcga cgccgatccg
     4861 cagcggcgac gtgctgttcg ggatcattta catcgattcg gaggaccgct ccgtcacgta
     4921 tgattttgcc gcgttgcgga cgctgtatct gatcgccgat cagctcggcg cgctgatgcg
     4981 gaaccgtccg ctgcacttcc ccaaacatcc ggtcgcgcgc cagcagatcg cccccgcgct
     5041 gggttcctga ccgaggtgga ggccgggtcg accgtcgccg cctggggtca ggggctctcc
     5101 atgctggtca tcctgacggg gcacggcgcg gcgctggtgc agtggggccg cacgccgccg
     5161 ctgcggtcgg ggacggtccg gcgcgccgcg ctgacatggg gctgcgccgc gatcgggctg
     5221 gtgacggcgc tggtggtgct gcgctggtcc cttgagccgg cgctgtcgct gtggtgtgtg
     5281 caggcggtgg ggctggcgct ggtgatccgg atgggcgcgt actgggcggc gcgcacgggc
     5341 gtggagccgt tcgggctgtt cggcgcgaac tggcgcgcct ggcccaagcg ctcgctggcg
     5401 ctgcggctgg ccgcgatgct gctgtggaca gccttgatgc tgccgttcgc cggcgcgggc
     5461 gggagcgtcg gctacgcgcg gcagctcggg ctggacgcgc tgtcgccgtg gcggattggg
     5521 gcctacgttc tgggggtatt gacgctggcg ccgatcctgg aggaggggtt cttccgccac
     5581 tacctgctcc atgggttgac ggcctggctg gggccggggg cgtggggcac ggcgggggcg
     5641 gcggtagtga ccagccttgc ctggtcggtc gggcatctgg ggctggtcga accgtggggg
     5701 ctgcgcctgc tgcacgtctt cggactgggg ctgctgctgg ccgacacgtc gcgccggcac
     5761 ggtctggacg ccgtcatcgc gctgcactgg ctgtttaatc tggcgctgat tccgctgttg
     5821 ttcgcgctgg gggactaagg ctttccgatg gaaaatcgct gatgacgtgg ccggacgcac
     5881 cgctgctgct cgcgctggac gccccgccgg cgccgggacg gccggaggag gaggtcctgc
     5941 agccagggtc tcccgaggcg cgggtgatcg tgggcgtgga ggacgaggcg gcggagctcg
     6001 gccgggcgga tatcctcctg acgtggcgtc gggcggtgga tgcggcgctg ctggagcgcg
     6061 ccgcgcgttg ccggctggtt ctgcactacg ggccgggggc cggcgcgggc gtcgggcggg
     6121 tggaccgcga tgccgcccgg cggcttggga tatacgtggc gagcatcccg gattacgccg
     6181 cggaggagtg ggcggagggg atgtgggagc tgaatgccgc gctggaggcg cgccggccgc
     6241 cggcgccgcc gttccgccgg cggccgcggg tcgggctgat cggtctgggg caggttgggc
     6301 ggcggcttgc cgagcggtgg aagcgcgccg cgggggaaat ctgggcgcat gatccgtacg
     6361 tcgacgacga tgtgtttgag cgggcggggg cgcggcgggc ggagctgacg gacgtgttcg
     6421 gcatctgcga tcaggtgctg attcagctct ccccggcccc gggggcgacc ggtttgatcg
     6481 ggcgggacat gcttgaactg atgcggccgt ggaggcaagt ggtgtgcggc tcgcggcccg
     6541 aggtctttgt ggaaacggaa ctccggtgga tgcttgaacg cgggcggccg ggcgggctcg
     6601 gcgtgctgga gccgatgctg gcgggggcgg cggcgtggga ctggctgcgt gcgcgggagc
     6661 gggtggttgt ccatgcgttc ggggaggagc tgaaccgccg catgtggcgg agtggccggc
     6721 gtcgggccgc ggaggtggtg ctgacgatgc tgcgcggcgg gcgaccgggt catctgctga
     6781 tcgacccgcc ctgcccgcgg catgagatgt ggggatgagg ggggatcgag aattctggat
     6841 cacgaaagac acgaacgaac acgaaaagct ttgggtggac gtgggacgtg ttggaatcag
     6901 gtgattgagg gggattgctg gcgttcggag aggtgctccc agcgggtcat ggcgtcgtcg
     6961 atggccgttt gcaggacggc ctgttcctcg ctcagtttgc gcaagcgttc gtaatcggtc
     7021 gccgcttcgg ccatggcctg gtgcaaggcg gcgaggcggg cctccatccg ggggatttcc
     7081 tcctcaagcc gttccagttc gcgctgttcc ttataggtca gtctggcgga aggggcagcg
     7141 gggcggcgcg cggggggcgg gcggtcggcc ttggcctcgg cgcgggcctc gcggcggcgc
     7201 gcctgctcct cttcctcgcg cagggcgcga taggtggagt aatcgccggg ccagaggcgg
     7261 gggacgccgt cgggttcaag cgaaaggacg cggtcgatcg tgcgatcgag aaagaagcgg
     7321 tcgtgcgaga cgacgagcag gcagccggcg aagccatcga ggaaatcctc gagcgcgttg
     7381 agcgtgggga ggtccagatc gttggtcggc tcgtcgagga tcaggaagtt ggggtcgcgc
     7441 aggagggtgc ggaccagata gaggcggcgg cgctcgccgc ccgagagctt ggcgatcggg
     7501 gcatgctgca tctgcggggt gatgccgaag cgttcgagca tcacgggcgc ggtcaggacg
     7561 gcgccgtcgg gttggcgcag catgtggccg ccctcgcgca ggacgtaatc gagcgttttt
     7621 tcggaggggt cgaggtcggc ggtttcctgg tcgtaatagc cgaagtggac cgtcgggccg
     7681 acgatgacct ggccggcctg gggtgcggcg cggccggtga ggacattcag cagggtagac
     7741 ttgccggagc cgttggggcc gatcagaccg aggcgttcgc cgcgcgtgaa ggtgtaggag
     7801 aaattccgga tgaggacgtg atcgtgataa ccggcctgga tttcgcgcag ttcgacgatc
     7861 ttcttgccta ggcggcggcc gcggacggcg aaggtcagtt cgtcggggga ggcatcgggc
     7921 gcgcgctcgc ggagggtttc gatgcggtcg atgcgggcct tctgcttggt ggtgcgggcg
     7981 cgcgcgccgc ggcgcagcca ggcgagctcc ttgcgcagga tcgtgcgccg gtggtcttcc
     8041 aggcgagcct cggcttcctc gcggcgcgcg cggttggcga ggtaatcgga gaaatgccct
     8101 tcgtggacga agatgcgccg gttggcgatt tcccagatgc ggttggtcac gcgatcgagg
     8161 aaatagcggt cgtgggtgac gaggaggatc gcgccgggga aggcgtcgag ttcctcctcg
     8221 agccactgga cggtatcggc gtcgagatgg ttggtgggct cgtcgaggat caggagttcg
     8281 ggctcgccga ggagggcggc ggccagggcg atgcgccggc gctcgccgcc ggagagttcg
     8341 gccgccgggc gctcccaagc gtcgagcccg aggcgggtca ggattgtgcg ggcggcggtg
     8401 tcgcgttccc aggcggcttc ggcctccatg cggcggccca agcgatcgac ctggcgctgg
     8461 atggctgcgt cgccggggtg ttcggccagg cggcggcagg cgagctcata gtcgtggatc
     8521 agttggctga gcggggaatc ggtgaagacg cagtccagaa tcgtgcgccg cggagggaag
     8581 cggggatcct gaggcatcag gccgatgcgc aggtcctggc gccaggcgac ctggccggcg
     8641 tcggggggct cgagccgggc gaggatgcgc agcagggtgg tcttacccga gccgttgggg
     8701 ccgatgatgc cgatgcggtc gcgctcgtcg acgccgaggg agagatcggc gaacaggggt
     8761 ttggcgccgt ggcttttggc cagattgttg accgagatga tattcacgcg ccggcgcgac
     8821 tttcctgcgc ccggccggcc tggcggacca tcaggtattc catcaggttt tcggcgttga
     8881 tgtagccgac gaggcggtcg ttctgaaaga ccaggagggg gaggttgccc gcatcgcgca
     8941 tccgctcgaa ggcggtctgg agcggctcgc cgggggtcgt gcgcgggaag tcgcgggtca
     9001 tgacgtgggc gacgtaatga tcgcgcccat ggagcgcgag gccctggatg agctcgttgc
     9061 gggtgagcag gccgatcaca tcggagccgc tcatgacggg gaaatcgtgc tggctggtcg
     9121 agaggagcag atcggcggcg cggccgaggc tgtcgccgtg atggaggacc tcgaaatggc
     9181 ggatcatcgc gtcctcgacg tggtggccgg acatcatcga gaccgactgg gtggcggcgt
     9241 attcctggcc ggcggcgaag aagatgaaca caccgatgaa gagcaggatc gggttgaacg
     9301 gggccagcag gaggaagccg atggcgagaa cctggccgat tttggcggcg atcgcggtgg
     9361 ctttttcgcg cgaggtggcg agcgtgagca gcgagcgcag gacgcggccg ccgtccatcg
     9421 ggaaggccgg gatcaggttg aagagcatca ggagcagatt gatcagcagc agttgctggg
     9481 cgaaggggat gcccgccagg cgggaatcgt cggtgacggg gacgaagccg ccggttgcga
     9541 ggaggaccag gccgagcgcg aaggcgatca gcaggttgac ggcgggcccg gccagggcga
     9601 tccacaattc ctggaggccc tcgcccatgc tgcgcagccg ggcgacgccg ccgatggggt
     9661 agaggacgat atcgtcggtg cgaatgccga agcggcgggc gacaagagcg tggcccagtt
     9721 cgtggaggac gatgctggcg aagacggcga ggaggaagag gacgccgccg agcagcgtgg
     9781 cgccgcggaa gatttcgaca taggccgcga agccgatcag caggagccag gtgatgtgaa
     9841 catagatcgg aatgccgaag accgtgagga gatagacgga ccaggggcgg tgggggcggc
     9901 ggtcggattg gctgccgggg gaaatgtcgg tggctcgggg catgggacgg aaatttgaat
     9961 gatccttgcg gtgatgcgcg agttcaaagc tcgcacgcca ttgtagcaga aggcaagaat
    10021 cctgccatgg ctgggatggg gtggaggggg aatcggggaa agatggacac gatggacgac
    10081 atggacgttg cggctgtggg aagagctgtc gggggcttta cgcgagccga gagccgaatt
    10141 ttggggcgga
//
LOCUS       DAOPCO010000143        10042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6458_length_10042_cov_6.013518, whole genome shotgun sequence.
ACCESSION   DAOPCO010000143 DAOPCO010000000
VERSION     DAOPCO010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6458_length_10042_cov_6.013518"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLS90_10590"
     CDS             complement(<1..296)
                     /locus_tag="PLS90_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013883691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPK02890.1"
                     /translation="MPSRKDRDPKLRGEAAPARKPRRDPAPAGELIRPDDDWRELVRG
                     LEGDPIAEETETVPSRAERLHPDWLRATRALPMVAVVGRPNVGKSTLFNRIIGRR"
     gene            428..1396
                     /locus_tag="PLS90_10595"
     CDS             428..1396
                     /locus_tag="PLS90_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPK02891.1"
                     /translation="MDPLIIFLWHPLSEEGAARLRALDPRVHLISPADVEADPDLVER
                     IEVVYGHLPDPWWPRATRLRWFQSMAVGVDRLVQRPEFRDREIMLTNTRGIQTPAMVE
                     HAFALLLAWIRQLHMAREAQLACRWEHYGVVEERAQVLMLCGRTLGLLGVGDIGGAAA
                     RVGRALGMKTIGCRRTGAPHPDLDRIFRPDQLAAFLAEADVVLNTLPATPGTRGIMNR
                     DAFAAMKPGAIYVNVGRGASDDTDAMLAALASGRLRAALLDVTNPEPLPPEHPLWRMP
                     NVLLTSHYGGLRPDSHAARERLLVENTRRYLAGEALLNLVDLNEGY"
     gene            1584..3287
                     /gene="tssA"
                     /locus_tag="PLS90_10600"
     CDS             1584..3287
                     /gene="tssA"
                     /locus_tag="PLS90_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HPK02892.1"
                     /translation="MAIDIAAIPALGVEPIAPDSPAGENLQDDFKFDQLQADVQALIN
                     QLGTTAEMDAVGDREPPKWENVALQAFDMLKTRSKDIRIASFLTIATAHVQGYSGLAA
                     CIEMVSGLLTNFWDTMYPPLKLLRGRVNTLGFIATHVAGESDEPGGLITLRVAQLREE
                     LDEAKSPSASLDAQRLAVEKMTAELEALKACQEQIGKLRDLIYEKFPQDKLPAVTSLT
                     DAVKAALPELEQLVAQNKPREPAAPSAPAAGGEPRPAAAASRPAPSAAAISAESPEEA
                     FKAMGRLAPMIRQADATHPAAYRLSRWAAWSALTMLPPTTDGVKTRVPEPPKQEIDQC
                     NALLASANWMALLNQSEERMQTTPLWLDRQFHVYSALGGLGGAYKAAQEAVRGEVANL
                     LRRLPGLQNLAFAGGMPLADAATQNWIQDVVLADSGGGGGGSARAGALSTDGADGLGE
                     ALDDARSLASEGRLADALRKLQEGARSRDSGRARFLWRMAQAQLAADNGKAKQMAPVW
                     EDLVGEIDRRQLESWEPDLCLAVFRALWQATRGDKERAELNQEMHRRLCRLDPAMALE
                     G"
     assembly_gap    3504..3513
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3514..4718
                     /locus_tag="PLS90_10605"
     CDS             <3514..4718
                     /locus_tag="PLS90_10605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK02893.1"
                     /translation="LARSAPDWMIDLRFTHVDMDPAEAFGMVGVEQPPNLRVSTVFPR
                     ESSAHLTTDQISQVLNHMPGRNNERLFEEWKREWQGRIAGRRAVLYTRNPPFLRLFRE
                     MPLKNFPFFVEIHQLRYRAKVKASPDARPSEIKPLLRKARDTEFDLISLAEKAWFISG
                     SMERIARRIRPNLNTGVLRSGYEPDRELARELTDPPDLSDEARDIDLLYSGQLYDWKG
                     VHVAIQALRYCNPRIRLTIVGGNNAGDLSINQHIAEHLGVADRIDWLGHRPRGEVMRL
                     QRRAKLSLVPLTRYFRISKWFTSPIKAFELMAAGTPMLVADLPTLRDLLEEDQTGAFA
                     RPNDPRDWARQIERVLADAPYRRRLAENGLRKIRDFTYDRRAATMRDEIQAWIDRQPD
                     KEAVALCG"
     gene            4709..5953
                     /locus_tag="PLS90_10610"
     CDS             4709..5953
                     /locus_tag="PLS90_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02894.1"
                     /translation="MRLIQSLIGWRQRRRAALGSPQSVWLILFGEIGDQVAALLPSPL
                     AVAMAKWAAPSGNGHPALHRLQLVIVTDRPATAQWLAAQGYIALDLSGHDAVPHDEMA
                     GEAMRRLAEPLRAHPPAAVVYTPEFALRFRRPIELATALRRYLLDLGRRQATETFTLL
                     TTDVDTEIHNAVDVARALKNPKGARGLPLRLLHYGSAQMYDYHRRLTFLPERTVESLG
                     WRLAESLLSRRRRGAGRHAFIWLRRPLARRDLLADLLPLRGRLGLSRLVVLGEPHVTQ
                     PRTPEVEYVAAAGPFLRRALTEAALVVADGDVPLFPFLQAGRPVALIESASQSKPALI
                     RCAYFMGEDRLPVFEHERDLLAALRAAESIPAFWRPWADKQSTIEREAPFLFQSALID
                     VLREMVATELARTEAAVPQPTG"
     gene            complement(6181..6552)
                     /gene="xseB"
                     /locus_tag="PLS90_10615"
     CDS             complement(6181..6552)
                     /gene="xseB"
                     /locus_tag="PLS90_10615"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPK02895.1"
                     /translation="MARRKGDSQPGFEQSLKQLEEIVRRLEEEEVPLETSLQLFADGQ
                     RLVRACEAQLKAAENQVRQLLENAAGEIEEQAFDATDDRCGELAGEAGEAESEDGALA
                     GEPAEAPPTSPTHPDDDDLPF"
     gene            6739..7395
                     /gene="plsY"
                     /locus_tag="PLS90_10620"
     CDS             6739..7395
                     /gene="plsY"
                     /locus_tag="PLS90_10620"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPK02896.1"
                     /translation="MPIAFPLLGILTAYLIGSLPTAYLMGRVKGIDIRRAGSGNVGAT
                     NALRVLGKPWGFGCLLVDLLKGYLPAAFLLPAAWFAPAWPDRLWPWVVGLAAVAGHTC
                     SPWLGFKGGKGVATSLGMLLAVIPLAMLILLPIALGIIAATGYVSLAAIVSCVLLPLI
                     LIAIDFRNPPWISVAVTSLLGLFIIWKHRGNIARLRAGTESRIFHRSSPHTSESGHAP
                     "
     gene            7392..8435
                     /locus_tag="PLS90_10625"
     CDS             7392..8435
                     /locus_tag="PLS90_10625"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPK02897.1"
                     /translation="MSLISPVTQDESRRFAVLGAGTWGITLADLLARNGHQVRCWDID
                     KSLLSRIGFDRTHPRLYNFAVSPEIQFLPDLAEAIDAVDACVVVAPSKAMRTLCEDLA
                     ATGKADAVGGWVLCNKGIEEDSLLLMNQVLADVLGEAAGRRAVILSGPSHAEEVCKGM
                     PTTLVASSPDPELARRIQKAFFRPYLRIYTHDDVHGVELGGSIKNVIAIAAGVNDGMG
                     YGDNARAALITRGLAEIVRLGLAMGARLETFMGLSGVGDLIVTAGSRHSRNYQFGRLI
                     AEGVTPDDALERIGMVVEGYPTARSAWGLARRHDVEMPITQAVYQICYEDLPPRQAVE
                     DLMLREPKPEHYR"
     gene            8467..9267
                     /gene="deoC"
                     /locus_tag="PLS90_10630"
     CDS             8467..9267
                     /gene="deoC"
                     /locus_tag="PLS90_10630"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPK02898.1"
                     /translation="MRDSIQTIARIREELAAVIQPEPPALTHPALIPPLDPDSVNRLT
                     PAELARFLDSTLLKPEATLEQIDALCDEARRLHVAAVCVHPTRLAQCIDRLAGTPVRT
                     ATVVGFPLGAMTSAAKAFETVLAVEAGADEIDMVLNLGWLRDGDDAAVRDEIRAVVEA
                     ARGRIVKVIVESGGLSDEELIRACLLARDAGAHFVKTSTGFLFGGAEAAAVALMRAAV
                     GPLMGVKAAGGVRDAAAARAMLRAGATRIGTSSARVIVDSAPAPAGPS"
     gene            complement(9272..>10042)
                     /gene="mazG"
                     /locus_tag="PLS90_10635"
     CDS             complement(9272..>10042)
                     /gene="mazG"
                     /locus_tag="PLS90_10635"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPK02899.1"
                     /translation="QTHQTLRQYMIEEAYEAAEAIEEGDMEALCEELGDVGLQVVFHA
                     ELAERAGVFRIEDVYDRICNKLIVRHPHVFGDVHAPDAEAVLRNWEQLKKRHKREKAE
                     RAGEGGHASAISGVPRSLPALQRAARLQDKASHVGFDWEKAEDVAAKVQEEVEEFLTA
                     ARTAGQGDAGAEETGRGMEEEFGDLLFSLVNLSRFLGIHAEESLQRACEKFRRRFTSV
                     EQAADAAGRPLSELTLAEMDRLWDEIKAREKNGGGPTA"
BASE COUNT         1525 a         3782 c         3053 g         1672 t
ORIGIN      
        1 cgccggccga tgatccggtt gaacagcgtc gacttgccga cgttgggccg cccgaccacc
       61 gcgaccatcg gcagcgcgcg cgtcgcccgc aaccagtccg gatgcagccg ctcggcgcgc
      121 gagggaaccg tttcggtctc ctccgcgatc gggtcgccct ccagcccgcg aaccagctcg
      181 cgccagtcgt catcgggccg gatcaactcg ccggcgggcg ccgggtcgcg gcgcggcttc
      241 cgcgccggcg cggcttcccc cctcagcttc ggatcgcgat cttttcggct tggcacgcgc
      301 atcgtgccca tagcttaaag gagttcccta ccccgcgcca gcgcgaacgg tcacccttga
      361 ccttccctcc gttcctctct tgaatggagc ggcgcaccga catcgcatcc cgacatggac
      421 gagcccgatg gaccctctga tcatcttcct ttggcatccg ctttcagagg aaggagcggc
      481 ccggctgcgc gcgctggacc cacgcgtcca cctgatctcc ccggccgacg tcgaggccga
      541 tcccgatctg gtcgagcgga tcgaggtcgt ctatggccat ctgcccgacc cctggtggcc
      601 gcgcgccaca cggctgcgct ggtttcagtc gatggccgtc ggcgttgacc gcctggtgca
      661 gcgtcccgag ttccgcgacc gcgaaatcat gctgaccaac acccgcggca tccagacgcc
      721 ggcgatggtc gagcatgcct tcgcgctgtt gctcgcctgg atccgccagc tccacatggc
      781 gcgcgaagcc cagcttgcct gccgctggga acattacggc gtggtcgagg agcgcgccca
      841 ggtcctcatg ctctgcggcc gaacgctcgg cctgctgggc gtgggcgata tcggcggcgc
      901 ggcggcgcgc gtcggccgcg cgctcggcat gaaaacgatc ggttgccgcc gtaccggcgc
      961 accccatccc gatctcgacc gcatcttccg gcccgaccag ttggcggcgt ttctggccga
     1021 agccgatgtg gtcctcaaca cgctgcccgc gacgcccggc acgcgcggga tcatgaaccg
     1081 cgacgccttc gccgccatga agcccggcgc gatttacgtc aatgtcgggc gcggcgccag
     1141 cgacgacacc gacgcgatgc tcgccgccct cgcgtcggga cgcctgcgcg ccgccctgct
     1201 cgacgtcacc aatcccgagc ccctgccgcc cgagcatccc ctctggcgca tgcccaacgt
     1261 cctgctgacg tcgcactacg gcggcctgcg ccccgactcg cacgccgccc gcgagcggct
     1321 gctggtggaa aacacccggc gctacctcgc cggcgaagcc ttgctgaacc tggtcgacct
     1381 gaacgagggc tattagcacc ccctcctgcc tccacttcgt ctctcgtcct cgtcctcgcc
     1441 ccaacggctt tccccgacga aagattgagt tttcgctcat tgaaccggat aatgtcgctt
     1501 cttgaggacc tcactggaga ccgccgccgc gtctcgccag ggcgggacga cgcggcctga
     1561 ttacttttcc cacggtgaac agcatggcca ttgatatcgc cgcgatcccc gctctgggcg
     1621 tcgagcccat cgcgcccgac agcccggcgg gggagaacct tcaggacgat ttcaaattcg
     1681 atcagctcca ggccgacgtg caggcgctca tcaaccaact ggggacgacg gccgagatgg
     1741 atgccgtcgg cgaccgcgaa ccccccaaat gggaaaacgt cgccctccag gcgttcgaca
     1801 tgctcaagac ccgctccaag gatattcgca tcgcctcgtt cctcacgatt gccacggccc
     1861 acgtccaggg ctactcgggc ctggccgcgt gcatcgagat ggtcagtggt ctgctgacga
     1921 atttctggga cacgatgtat ccgccgctga agctcctgcg cgggcgcgtc aatacactcg
     1981 gcttcatcgc gacgcacgtc gccggcgaga gcgacgagcc gggcggcctg atcacgctgc
     2041 gcgtcgccca gttgcgcgag gaactggacg aggcgaaatc ccccagcgcc tcgctcgacg
     2101 cccagcgcct ggcggtcgag aaaatgaccg ccgaactgga ggcgctcaag gcctgccagg
     2161 agcagatcgg caaactgcgc gacctgatct acgaaaagtt cccgcaggac aaactgccgg
     2221 cggtcaccag cctgaccgac gcggtgaagg ccgcgctccc cgagctggaa caactggtgg
     2281 cccagaacaa gccgcgcgag ccggccgccc ccagcgcccc ggccgccggc ggcgagcccc
     2341 gcccggccgc ggccgcctcg cgcccggccc ccagcgcggc ggcgatcagc gccgaatcgc
     2401 ccgaggaagc cttcaaggcc atgggccggc tcgcccccat gatccgtcag gccgacgcca
     2461 cccacccggc cgcctaccgc ctcagccgct gggcggcgtg gtccgcactg acgatgctgc
     2521 cgccgacgac cgacggcgtc aaaacgcgcg tccccgagcc gcccaagcag gagatcgacc
     2581 agtgcaacgc cctgctcgcc tcggccaact ggatggcgct gctcaatcag tccgaggaac
     2641 ggatgcagac gactccgctc tggctggatc ggcagttcca tgtctactcg gccctgggcg
     2701 gcctgggcgg cgcctacaag gccgcgcagg aggcggtccg cggcgaagtg gccaacctgc
     2761 tccgccgatt gccgggattg cagaatctgg ccttcgccgg cggcatgccg ctggccgacg
     2821 ccgccacgca gaactggatc caggatgtcg tcctggccga tagcggcggg gggggcggcg
     2881 gctccgcccg cgcgggcgcc ctctcgaccg acggcgccga cggccttggc gaggcgctcg
     2941 acgatgcccg ctcgctcgct tccgaaggcc ggctggccga cgccctgcgc aagctgcagg
     3001 agggagcgcg cagccgcgac tcggggcgcg cccgcttcct ctggcgcatg gcgcaggcgc
     3061 agctcgccgc cgacaacggc aaagccaaac agatggcgcc cgtctgggag gacctggtcg
     3121 gcgaaatcga ccgccgccag ctcgaatcct gggaacccga cctctgcctg gcggtcttcc
     3181 gcgcgctgtg gcaggcgacg cgcggcgaca aggagcgcgc cgaattgaac caggaaatgc
     3241 accgccggtt gtgccggctc gatcccgcga tggcgctgga gggttaactc tccgccgccg
     3301 cttcgaaaaa aatgatatcg aaatcggtat ccccatgatc gtgcggtccg gcccgcaaga
     3361 tcacgattga cggattacat cccggcgagg aaactccgct tagttaggga gcgttgtcgc
     3421 gcatgtcccc ctttgacctt gcgccggata tcgaccgcta tcagcgtgta gtcttcgttc
     3481 ttgcctttac cccgctgctg ccgnnnnnnn nnncgctggc ccgctccgcc cccgactgga
     3541 tgatcgatct gcgcttcacc catgtcgaca tggacccggc tgaggccttc ggcatggtcg
     3601 gcgtcgagca accccccaat ctgcgcgtca gcaccgtctt cccccgcgaa tccagcgctc
     3661 atctgaccac cgaccagatc agccaggtgc tcaaccacat gcccggacgc aacaacgagc
     3721 gcctgttcga ggagtggaag cgggaatggc aggggcgcat cgcgggacgc cgcgccgtgc
     3781 tctacacgcg caacccgccg tttctgcgtc tcttccgcga gatgccgctc aagaacttcc
     3841 cgttcttcgt cgagatccat caactgcgct accgcgccaa ggtcaaggcg tcccccgacg
     3901 cccgcccctc ggaaatcaag cctctgctgc gcaaggcgcg cgataccgag ttcgatctga
     3961 tcagcctcgc cgagaaggcc tggttcatca gcggctcgat ggagcgcatc gcgcgccgca
     4021 tccggccgaa cctcaacacc ggcgtcttgc gcagcggcta cgaacccgac cgcgaactgg
     4081 cccgcgagct gaccgacccg cccgacctga gcgacgaggc gcgcgacatc gatctcctct
     4141 acagcggcca gctttacgac tggaagggcg tccacgtcgc catccaggcg ctgcgctact
     4201 gcaaccccag gatccggttg accatcgtcg gcggcaacaa cgccggcgat ctttcgatca
     4261 accagcacat cgccgaacac ctgggcgtcg ccgaccgcat cgactggctc ggtcatcgcc
     4321 cccgcggcga ggtgatgcgc ctgcagcggc gcgccaagct cagcctggtc cccctgaccc
     4381 gctatttccg catctcgaag tggtttacca gcccgatcaa ggcctttgaa ctgatggcgg
     4441 ccggcacgcc gatgctcgtc gccgacctcc ccaccctgcg cgatctgctc gaggaggatc
     4501 agaccggggc tttcgcccgc ccgaacgatc cgcgcgactg ggcccgccag atcgaacgtg
     4561 tcctggcgga cgccccctac cggcgccgcc tggccgagaa cggcttgcgc aagatccgcg
     4621 acttcaccta cgaccgccgc gccgcgacga tgcgcgacga gatccaggcc tggatcgatc
     4681 ggcaacctga caaagaggcg gtggcgctgt gcggctgatc caatccctga tcggttggcg
     4741 acagcggcgc cgcgccgccc tgggcagtcc ccagtccgtc tggctgatcc tcttcggcga
     4801 gatcggcgat caggttgccg cgctcctccc ctcgcctctg gccgtcgcga tggccaagtg
     4861 ggccgcgccc tccggcaacg gccatcccgc cctgcatcgg ctgcagctgg tcattgtgac
     4921 cgaccgcccc gccacggccc agtggctcgc cgcgcagggc tacatcgcgc tcgacctgag
     4981 cggtcacgat gccgtcccgc acgacgagat ggccggcgag gcgatgcgcc ggctcgccga
     5041 gccgctgcgc gcccacccgc cggcggccgt cgtttacacc ccggaatttg ccctgcggtt
     5101 ccgccgcccg atcgaactgg ccacggcact gcgtcgttac ctgctcgacc tgggccgtcg
     5161 ccaggcaacc gaaacgttca cgctgctgac caccgacgtc gacaccgaaa ttcacaacgc
     5221 cgtagatgtc gcccgcgcgc tgaagaatcc caagggcgcg cgcggcctcc ccctccgcct
     5281 gttgcattac ggctcggcgc agatgtatga ctaccaccgg cgcctgacat tcctccccga
     5341 acggacggtg gaatcgctcg gctggcgtct ggccgagagc ctcctgtccc gccgccggcg
     5401 cggcgccggt cgccatgcgt tcatctggct gcgccggccg ctggcccgtc gcgatctgct
     5461 cgccgacctg ctgccgctgc gcggccggct cggcctgagc cggctcgtcg tgttgggcga
     5521 gccccacgtg acccagcccc gtacgccgga ggtggaatac gtggccgcgg cggggccgtt
     5581 tctgcgccgc gcgctgaccg aagccgccct cgtggtcgcc gacggcgacg tgcccctctt
     5641 cccgttcctg caggccggcc gcccggtggc cctgatcgag tcggcctcgc aatccaagcc
     5701 ggcgctgatc cgctgcgcct atttcatggg agaggaccgc ctccccgtct tcgagcacga
     5761 gcgcgacctg ctcgcggcgc tgcgggccgc cgaatcgatc cccgccttct ggcgcccctg
     5821 ggccgacaaa caatccacga tcgagcgcga agcccccttc ctcttccaga gcgcgctgat
     5881 cgacgtcctg cgcgaaatgg tcgccaccga actggcacgg acagaggccg ccgtccccca
     5941 gccgacaggc tgagccgcgg caatgtctac caatcaaaga atcctacgcc aaaatttttg
     6001 tctttcgctt tgatgttctt gcgtcttcac gaagtatttt ttcctgattt ctctgtgggc
     6061 cttgatttct tccgcttttg agatttgagc cttcggattt gtttcgaatt tcgagtttcg
     6121 aatttcgcgc ttcctccgcg cctccgcgtg agccagctta atccaatctg tgacctgctg
     6181 ctaaaacgga agatcgtcgt cgtcgggatg ggtcggggac gtcggcggag cttccgccgg
     6241 ttcgcccgcc agtgccccat cctcgctctc cgcctcgccc gcttcccccg ccagctcgcc
     6301 gcagcggtcg tccgtcgcgt cgaacgcctg ctcctcgatt tcaccggcgg cattctccag
     6361 caactgccgc acctgattct cggctgcctt caattgcgcc tcgcacgcac gcaccagccg
     6421 ctggccgtcg gcgaagagtt gcaacgacgt ctccagcggc acttcttcct cctccagccg
     6481 ccgcacaatc tcttccagtt gcttgagcga ctgctcaaac cccggctgtg aatcgccttt
     6541 cctgcgcgcc atcgcgtacc tgctccttca ttgcggcgtc cggtcgaccg ttgttagatc
     6601 agccgtccca ttgtgaaacc tgcccgaatc ggcgacaagg cccttttttc gcttacgaac
     6661 cgccccgcgc cttgcctaag atacttgcgg cccgcgcgct gccttcgcat ccccaacccg
     6721 ccgcccggac tcgtcgccat gcccatcgcg ttccccctcc ttggcatcct gaccgcctac
     6781 ctcatcggtt cgctcccgac ggcctacctg atggggcggg tcaaggggat cgatatccgc
     6841 cgggccggca gcggcaacgt cggcgccacc aatgccctgc gcgtcctcgg caagccgtgg
     6901 gggttcggct gcctgctcgt cgatctgctc aagggctacc tgccggcggc gttcctcctg
     6961 ccggcggctt ggttcgcgcc ggcgtggccc gaccggctct ggccctgggt cgtgggcctc
     7021 gccgccgtcg ccggccacac ctgcagcccg tggctcggat tcaagggcgg caagggcgtc
     7081 gccaccagcc tcggcatgct gctcgccgtg atcccgctcg ccatgctgat cctgctgccg
     7141 atcgcgctgg ggatcatcgc cgcgaccggt tacgtctcgc tcgccgcgat cgtctcgtgc
     7201 gttctgctgc cgctcatcct gatcgccatc gacttccgca atccgccctg gatctcggtg
     7261 gcggtcacct cgctgctcgg actgttcatc atctggaagc accggggcaa catcgcgcgg
     7321 ctgcgcgccg gaaccgaatc gcgcatcttc catcgctcct ccccgcacac ctccgaatca
     7381 ggacacgcgc catgagcctc atcagccccg tcactcagga tgaaagccgc cggttcgcgg
     7441 tcctcggcgc cggcacgtgg ggcattacgc tggccgatct gctcgcccgc aacggccatc
     7501 aagtccgctg ctgggacatc gacaaatccc tgttgtcgcg catcggcttc gaccggaccc
     7561 acccgcggct ctataacttc gccgtcagcc cggaaattca gtttctgccc gacctggccg
     7621 aggcgatcga cgcggtcgac gcctgcgtcg tcgtcgcccc ctccaaggcg atgcgcacgc
     7681 tgtgcgagga cctggccgcg accggcaagg ccgacgccgt cggcggctgg gtcctctgca
     7741 acaagggcat cgaagaggac tcgctcctgc tgatgaacca ggtcctcgcc gacgtgctgg
     7801 gcgaggccgc cggccgccgc gccgtcatcc tgagcggccc gagccacgcc gaagaagtct
     7861 gcaaggggat gccgacgacg ctggtcgcca gctcgcccga cccggaactg gcgcggcgga
     7921 tccagaaggc cttcttccgc ccgtacctgc gcatttacac gcatgacgac gtgcatggcg
     7981 tcgagctcgg cggatcgatc aagaacgtga tcgcgatcgc cgcgggcgtc aacgacggca
     8041 tgggctacgg cgacaacgcg cgcgcggcgc tgatcacgcg cggactggcc gagatcgtcc
     8101 gcttgggcct ggcgatgggc gcccgcctcg aaacgttcat ggggctctcc ggcgtcggcg
     8161 atctgatcgt caccgccggc agccgccact cgcgcaatta tcaattcggc cgcctgatcg
     8221 ccgaaggcgt cacccccgac gacgcgctgg aacggatcgg catggtcgtc gaggggtatc
     8281 ccacggcgcg gtctgcgtgg gggctcgccc gccgtcacga cgtcgaaatg ccgatcacgc
     8341 aggccgtcta ccagatttgt tacgaagacc tgccgccgcg ccaggccgtc gaggatctga
     8401 tgctgcgcga accaaaaccc gagcactacc gttgaaacgg cacgggagac gcctcggcct
     8461 tcaatcatgc gggattccat tcagacgatc gcccgaatcc gggaggaact tgccgcggtc
     8521 atccagcccg agccgcccgc gctcactcat cccgcactca tcccgccgct cgatcccgat
     8581 tccgtcaacc ggctgacccc ggccgaactg gcccgcttcc tggacagcac actcctcaaa
     8641 cccgaggcga cccttgaaca gatcgacgcg ctgtgcgacg aggcacgccg cctccacgtc
     8701 gccgccgtct gcgttcaccc gacgcgcctg gcgcagtgca tcgaccgtct tgcgggcaca
     8761 cccgtccgca cggcgacggt cgtgggtttc cccctgggcg cgatgacctc cgccgccaag
     8821 gcctttgaaa cggtcctggc cgtcgaagcc ggcgccgacg agatcgacat ggttctcaat
     8881 ctggggtggc tgcgcgacgg cgacgacgcg gcggtgcgcg acgagatccg cgccgttgtc
     8941 gaagccgctc gcgggcgcat cgtcaaggtg atcgtggaat cgggcggctt gagcgacgag
     9001 gagctcatcc gcgcctgcct gctcgcgcgc gatgccggcg cgcatttcgt caagacgagc
     9061 accggtttcc tgttcggcgg cgcggaagcg gcggcggtgg cgctcatgcg cgccgccgtc
     9121 ggtccgttga tgggcgtcaa ggctgccggc ggcgtgcgcg acgcggccgc cgcccgcgcc
     9181 atgctgcgcg ccggcgcgac ccgcatcgga accagctcag cccgcgtgat cgtcgatagc
     9241 gcccccgccc ccgccggccc gtcgtgaatg atcaagccgt cggtcccccg ccattctttt
     9301 cgcgcgcttt gatctcatcc cacagccggt ccatttcggc gagcgtcagc tcgctgagcg
     9361 ggcggcccgc ggcgtcggcc gcctgctcca cggacgtgaa ccggcggcgg aacttttcgc
     9421 acgcccgctg gagcgattcc tcggcgtgaa tccccagaaa ccgcgataga ttgaccaggc
     9481 tgaacagcag gtcgccgaac tcttcctcca tcccccggcc ggtctcctcg gcgccggcat
     9541 cgccctgccc cgccgtccgc gccgccgtca ggaattcctc gacttcttcc tgcaccttcg
     9601 cggccacatc ctccgccttc tcccagtcaa agcctacgtg cgatgcctta tcctgcagcc
     9661 gcgcggcccg ctgcagcgcc ggcaggctgc gcggcacccc ggaaatcgcc gaggcgtgcc
     9721 ccccttcccc cgcgcgctcc gccttctcgc gcttgtgccg cttcttgagc tgttcccagt
     9781 tccgcaacac ggcttcggca tccggcgcat gcacgtcgcc gaacacatgc gggtggcgca
     9841 cgatcagctt gttgcagatc cggtcgtaaa cgtcctcgat ccggaagacg ccggcgcgct
     9901 cggcgagctc cgcatggaac accacctgca gcccgacatc gcccaattcc tcgcacagcg
     9961 cctccatgtc cccctcttcg atcgcctcgg ccgcctcgta ggcctcctcg atcatatact
    10021 ggcggagcgt ctggtgcgtc tg
//
LOCUS       DAOPCO010000144         9983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6493_length_9983_cov_4.671031, whole genome shotgun sequence.
ACCESSION   DAOPCO010000144 DAOPCO010000000
VERSION     DAOPCO010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6493_length_9983_cov_4.671031"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            481..1641
                     /gene="bshA"
                     /locus_tag="PLS90_10640"
     CDS             481..1641
                     /gene="bshA"
                     /locus_tag="PLS90_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HPK02900.1"
                     /translation="MHPKPLRIGIVCYPTYGGSGAVATELGRLLARRGHTIHFLSYAR
                     PFRLDGDFQENIFYHEINAENYPLFQGQLYTISAAVKIVEIVRNIGLDLIHLHYALPH
                     AISAWMAQEMLGEQRIPMLTTLHGTDITLVGSKPSFHAAVQLGLDKSDMLTGVSNWLR
                     AKTCELFRICNRIRVVYNFIDPELFQPARKKCPRRHFAADDEQIVMHISNFRPVKRVE
                     EVVRIFARMQREVKARLLLIGDGPDREKALAAAEELGIHDRVALLGKQPGVEHFLPLA
                     DLFLFPSTGESFGLAALEAMASGVPVVGCQAGGLPEVVIDGECGYLRACDDLDGMAEA
                     GLRVLRDAELRRAMGEAGRRRAIEEFAADKIIAQYEQVYREMLAGAPACPVG"
     gene            complement(1707..2369)
                     /locus_tag="PLS90_10645"
     CDS             complement(1707..2369)
                     /locus_tag="PLS90_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02901.1"
                     /translation="MPDRLKPDIAPEVHRREITLPPEGQLLDWDALFGRPAPRAIEIG
                     TGNGFFLAREAARLPDWTFIGVEREPLYYYKMARRIARAKLDNVRTWRGEALDLLRRV
                     PPGSLDRFYCYFSDPWPKRRHRRRRVYNQDFPPLIEQLLAPTGEVWFKTDADFYFNLT
                     VTLFRRRGGWDLLDVGRLPLPDPARGEVISNFERKAREAGREVWGFRARRGEGRLLPR
                     TG"
     gene            complement(2420..4033)
                     /locus_tag="PLS90_10650"
     CDS             complement(2420..4033)
                     /locus_tag="PLS90_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPK02902.1"
                     /translation="MTQPHPSDQPGALERRVTGGRFIALVLAIAIFSGIVTLAITALL
                     MNIQERKQEARNPFFRVVELDDMTVDPAVWGKNFPLQYDMYLKTVDMQRTRFGGSEAM
                     VRRPTDADPRTQVAQSKLEEDPRLATMWAGYAFAIDFREERGHAYMLEDQTYTQRHQK
                     EQAGTCLHCHASTYVPYKIAGDGDLIAGFEKFNPLPYFEARKQMETIMTETLDDPHGL
                     HPVACIDCHDPETMALRVTRPGFLEGIAAFKREVEGIDNYDPNTMATRQEMRTFVCGQ
                     CHVEYYFKGEGKRLTYPWTNGLRGDQILAYYEETGFADWTHAETGAQVLKAQHPEFEM
                     YNQGIHARSGVACADCHMPYVREGALKVSDHHVRSPLLNINNACQTCHNFPEAEMKER
                     AELIQSRFFEMRDLALDGLVELIAEIKAARQAGVGDEQLRAAQGFQRKSQFLIDFCEA
                     ENSMGFHAPQESARLLTQALDYVRQGQLALRGSVAATSGATGALPPSVESAPAPPQPM
                     QPAAPPENPPDEQSAQSAPASESAVFNEG"
     gene            complement(4064..4531)
                     /gene="nrfH"
                     /locus_tag="PLS90_10655"
     CDS             complement(4064..4531)
                     /gene="nrfH"
                     /locus_tag="PLS90_10655"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPK02903.1"
                     /translation="MAHWLTTRTALIAAVALGLAAGSAAGVGFYTFVYARGASYMTND
                     PTACVNCHVMRGQFEAWQRGSHHAVAVCNDCHTPHNLLGKWTTKALNGWHHSVAFTTG
                     QFHEPIQITERNRKITQQTCRDCHGDIVAMIDTHTRGERVDCIRCHREVGHGD"
     gene            complement(4758..6050)
                     /locus_tag="PLS90_10660"
     CDS             complement(4758..6050)
                     /locus_tag="PLS90_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPK02904.1"
                     /translation="MPASPQPPFRRGLRARAWRSLLLTALSLAAAGGCRQAGDDPVII
                     YLRMGSPIEQEIWGDMFAAISTKFPEIQVKVLNVPYEGYWSKFFTLTASGNPPDLVLM
                     ESGYYPGFVEKGALLDLTDLIAQSDVINLEAYHPTPLQWLTIDGRLYGPPSDTAIMIP
                     FLNLDQFEAAGVPVPGPDWTWEDYLEIARRLTRDTDGDGDIDVYGTAQLPWESVIWSF
                     GGDLVDDPAKPTRCTFDDPLTIKAIQWIADARLKEGVVASETMQTAQIDPFQNGQAAI
                     GWNGHWAVPEYTKRADFKWDVAMLPIGPNGRAIANFGSCFSIPTRARHPEEAWKLLEF
                     LSGAEGSRIFIDRNIMTPALRELAASPAYLDQRPPAHQEYFIAALDHCRSRPQTPAQN
                     ELYAMINMELELIWAGEESVQSVCERISAKGTKILNDR"
     gene            complement(6078..6458)
                     /locus_tag="PLS90_10665"
     CDS             complement(6078..6458)
                     /locus_tag="PLS90_10665"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK02905.1"
                     /translation="MYERVHHYGLFATMHMCGDNTEVIPDLIEVGLDCMESCQPECMD
                     LYDLKRRYGRDIRFWGGLGAQRMMPFGTPDEIRREIRRMKRELGRGGGFLLACAKAPD
                     AITPLANILAYLEEAGQPRPVDEL"
     gene            complement(6465..7136)
                     /locus_tag="PLS90_10670"
     CDS             complement(6465..7136)
                     /locus_tag="PLS90_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02906.1"
                     /translation="MKTIEATLQPRDVVKLAFQFQETEFVPYDDLMLYEHDQALSAWF
                     GDEHWREQHSVSYLGALTGFDGSLTLAGMTPQPDGTQKDILGCSWRMGNISHLEDWPL
                     HEPEMGDYRLPGLQPYFEQYVKPRWPREIAQSANRFRICWTVFGLFERAWTLRGFENF
                     LVDLALNEKFAEQLLDHITEYLLQSVDLMAQAPLDSICFTDDHACQRGMIMGADRWRR
                     LFKPR"
     gene            complement(7123..8808)
                     /locus_tag="PLS90_10675"
     CDS             complement(7123..8808)
                     /locus_tag="PLS90_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02907.1"
                     /translation="MTLRGICNALFLLAVAAFLPLAGFAADSADSGEFAYFPIRGAAQ
                     GFKWHKKLTDEPGLIEQARQMLDIGCDAFKTMVGPDVGGKGYRDVYPDMPELPREKKN
                     SMVRLVQNTPTYQEFFDLPFKFFVIWAWPTNDITFSWDWEQATFDEEYQQLYDFTRYL
                     METYQGTGKVFLLGNWETDHVLMSGLDQVADPKVQPSQERLEYLRRWLNNRQKAVEDG
                     RNSLPEIEGVEVYHYVEVITIQPVIEDPTLMRVVNGVLPYIRMDLVSNSAYNSTNHTS
                     DLPQRVYDHMDCILKNAEKSLTGAWPYGPPVFISEFGIPGGYTPQKGARNREALKACA
                     GWGSVINLFWEVYSQTPDNTDSALFQSDGTPNGNYEMLRDFNFRMHTLKNARRVWCRR
                     NPTEQEANNQARLYDTLTNADILRDSINLSSEIMSDEEFLKTLFTDLRLGDQIGQSWY
                     QELLAKLARADLQYGYNTRYATLGAILDSDLFAAAVPQEEFAAYLTRRVINQPVELPD
                     LGRRDLYEWALNHPGFDRGNLEYRLANNVPDSLRDKYSLKLQQFQEGSEHENH"
     gene            complement(8886..9767)
                     /locus_tag="PLS90_10680"
     CDS             complement(8886..9767)
                     /locus_tag="PLS90_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02908.1"
                     /translation="MNPTSQQHLPAASAAGLSFTAAGPEFHFDTGFLKGTLHPEGMSS
                     GLIPLTHSATGTELAGMFGLLSPYRLLTPDVRFGTAAWDWPSTAAVQQDGSVRVDWRP
                     DEEHPLEMTAVYRWRERNVADLRLTVRPHKNLHHFEVFLASYFNGFPVTSACVAANPD
                     DGGRRGFMEAPAANGGWQAFPRDEAATALFADGRWTLEPHPLPWVIMPRLAAPLAMRR
                     DPDTGLAALLMGRPEDCFAISMPENEDYHRSVYLSLFGRDFQAGEEATTRARLVIDAG
                     LSAEQAMALFDAFLRDE"
BASE COUNT         1705 a         2911 c         3560 g         1807 t
ORIGIN      
        1 cgtgccaccg gagccaaagc ggcgtcgcca agcgctgcgc gctttctgcc gccgcgctcc
       61 acaagggcct cctgttcgaa gggggcttcc agtctggatg acagacccag tctcgaatcg
      121 tgtccagacg cgccggggac aggcgggcgg ccgatcaaca cggctcgtgc caccggagcc
      181 aaagcggcgt cgccaagcgc tgcgcgcttt ctgccgccgc actccacaag ggcctcctgt
      241 tcgaaggggg gctatgcgtc atatctgaag gacaggccga ctcgcgaacg ggggcaggat
      301 gcgccgggga caggcggacg gtcgatcaac acggctcgtg ccagatgcgc cggggacagg
      361 cgggcggccc gtttttccgt ggccggcggt tgcgcgcccc gagtaggata tgatctgatt
      421 gccgcgacgg cgggggcacg cgagcgcccc tgcgccgcgg gagctttcag gattggcgcc
      481 atgcatccca aacccctgcg catcggcatc gtctgctacc ccacgtatgg ggggagcgga
      541 gcggtcgcca ccgagctcgg gcggctgctc gcccggcgcg gtcatacgat tcactttctc
      601 tcctacgccc ggccgttccg gctcgacggc gactttcagg agaatatctt ctatcacgag
      661 atcaacgcgg agaattaccc gctgttccag ggccagctct acacgatttc ggcggccgtc
      721 aagatcgtcg agatcgtccg caacatcggt ctggatctga tccatctgca ctatgcgctg
      781 ccgcacgcga tcagcgcctg gatggcccag gagatgctcg gcgagcagcg cattccgatg
      841 ttgacgacgc tccatgggac ggacatcacg ctggtcggct ccaagccgtc gttccatgcc
      901 gccgtccagc ttgggctgga caagagcgac atgctgacgg gggtgtccaa ctggctgcgg
      961 gcgaagacgt gcgagctgtt ccggatctgc aaccggattc gcgtggttta caatttcatc
     1021 gatccggagc tgttccagcc ggcgcgcaag aagtgcccgc ggcggcactt cgcggccgat
     1081 gacgagcaga tcgtgatgca catttccaac ttccggccgg tcaagcgcgt cgaggaggtg
     1141 gtgcggattt tcgcgcggat gcagcgcgaa gtgaaggcgc ggctgctgct gatcggcgac
     1201 gggccggatc gggagaaagc cctcgccgcg gccgaggaac tggggattca cgaccgcgtc
     1261 gcgctgctgg gcaaacagcc cggcgtcgag catttcctgc cgctggccga tctgttcctg
     1321 tttccgtcga cgggggaatc gttcgggctg gcggcgctgg aggcgatggc cagcggggtg
     1381 ccggtcgtcg gctgccaggc ggggggactg cccgaggtcg tgatcgacgg agaatgcggc
     1441 tacctgcgcg catgcgacga tctggacggg atggccgagg cggggctgcg ggtcctgcgc
     1501 gacgcggagc tgcgccgcgc gatgggggag gccggccgcc gccgcgcgat tgaggaattc
     1561 gccgccgaca agataattgc gcagtacgaa caggtttacc gtgaaatgct cgcgggggcg
     1621 cccgcgtgcc cggtggggta gccggcgggg ggaacgccgg gagaagagcg cctgcgcgag
     1681 cgggtggccg atggccgcgt gcccggttaa ccggtgcggg gcagcagacg gccttcgccg
     1741 cggcgggcgc ggaagcccca gacttcgcgg ccggcttcgc gcgccttgcg ttcgaaattg
     1801 ctgatgacct cgccgcgcgc ggggtcgggc aggggcaacc ggccgacgtc gagcaagtcc
     1861 cagccgccgc ggcggcggaa gagcgtgacg gtcagattga agtagaagtc ggcgtcggtc
     1921 ttgaaccaga cctcgcccgt aggcgcgagc agttgctcga tcagcggcgg gaagtcctga
     1981 ttgtagacgc ggcggcggcg atggcggcgc ttgggccagg ggtcgctgaa gtagcagtag
     2041 aagcggtcga gcgagccggg ggggacgcgg cggagcaggt caagcgcctc gccgcgccag
     2101 gtgcggacgt tgtcgagttt cgcgcgggcg atgcgcctgg ccatcttgta gtaatagagc
     2161 ggctcgcgct cgacgccgat gaacgtccag tcgggcaggc gggcggcctc gcgcgcgagg
     2221 aagaagccgt tgccggtgcc gatttcgatt gcgcgagggg ccggccggcc gaagagggcg
     2281 tcccaatcga gcagctggcc ttcggggggc agcgtaatct cgcggcgatg gacttcgggg
     2341 gcgatgtcgg gtttgagtcg gtcaggcatg ggttaacaac gtcgcaactc gtggttacgg
     2401 cagcggaatt tatagagatt catccctcgt tgaaaaccgc cgattccgag gcgggggcgg
     2461 actgggcgga ctgctcatcg ggcggattct cgggtggggc ggcgggttgc atcggctgag
     2521 gaggcgcggg tgcggattcg accgaggggg gcagcgcgcc cgtcgcgccg gaagtcgccg
     2581 cgacactccc gcgcagcgcg agctggccct gacggacgta atcgagcgcc tgggtcagca
     2641 gccgcgcgct ttcctgcggc gcatggaacc ccattgaatt ttccgcttcg cagaagtcga
     2701 tcagaaactg gctcttgcgc tggaagccct gggcggcgcg cagttgctcg tcgccgacgc
     2761 cggcctggcg ggcggccttg atctcggcga tcagctccac gaggccatcg agcgccaggt
     2821 cgcgcatttc gaagaaccgc gactggatca gctcggcgcg ttccttcatt tcggcctcgg
     2881 ggaagttgtg gcaggtctgg caggcgttgt tgatgttgag cagcgggctg cggacgtggt
     2941 ggtcgctgac cttgagcgcg ccctcgcgca cgtagggcat gtggcaatcg gcgcacgcga
     3001 cgccggagcg ggcatggatc ccctggttat acatctcgaa ttcggggtgc tgcgccttca
     3061 gcacctgggc gccggtctcg gcgtgggtcc agtcggcgaa gccggtctcc tcgtagtagg
     3121 ccaggatctg atcgccgcgc aggccgttgg tccagggata ggtcagccgc ttgccctcgc
     3181 ccttgaagta gtattcaaca tggcactggc cgcagacgaa ggtgcgcatt tcctggcgcg
     3241 tggccatcgt gttggggtca tagttgtcga tcccctcgac ctcgcgcttg aaggcggcga
     3301 tgccctcgag gaagccgggg cgggtgacgc gcagggccat cgtttcggga tcatgacagt
     3361 cgatgcaggc gaccggatgc aggccgtggg gatcatcgag agtctcggtc atgatggttt
     3421 ccatctgctt gcgcgcctcg aagtagggca gcgggttgaa tttttcaaag ccggcgatca
     3481 gatcgccgtc gcccgcgatc ttgtagggga cgtaggtgga ggcatggcag tgcaggcagg
     3541 tgccggcctg ctctttctga tgccgctggg tgtaggtctg gtcctcgagc atgtaggcat
     3601 ggccgcgttc ctcgcggaag tcgatggcga aggcatagcc ggcccacatc gtggcgaggc
     3661 gggggtcctc ctccagcttg gactgggcga cctgggtgcg ggggtcggcg tcggtgggcc
     3721 tgcggaccat ggcctcgctg ccgccgaagc gggtgcgctg catgtcgacg gttttgagat
     3781 acatgtcgta ctgaaggggg aagtttttgc cccagacggc cggatcgacg gtcatgtcgt
     3841 ccagctcgac gacgcggaag aaggggttgc gggcctcctg tttgcgctcc tggatgttca
     3901 tcagcagggc cgtgatggcg agcgtgacga tgccggagaa gatggcaatg gccaggacaa
     3961 gggcgatgaa acgcccgccg gtgacgcgcc gttccagcgc gccgggttgg tcggaggggt
     4021 ggggttgggt catgatggtc tctccggcgg agatggcggt ggttcagtcc ccgtggccga
     4081 cttcgcggtg acagcggatg cagtcaacgc gctcgccgcg ggtatgggta tcgatcatcg
     4141 ccacgatatc gccgtggcaa tcgcggcagg tctgttgggt aatcttccgg ttgcgctcgg
     4201 tgatctggat cggctcatgg aactggcctg tggtgaaggc gaccgagtga tgccagccat
     4261 tgagcgcctt ggtcgtccac ttgccaagga gattgtgcgg ggtgtggcaa tcgttgcaga
     4321 cggcgacggc atggtgcgag ccgcgctgcc aggcttcgaa ctgaccgcgc atgacgtggc
     4381 agttgacgca ggcggtcggg tcgttggtca tgtaggaggc cccgcgggcg tagacgaacg
     4441 tgtagaagcc gacgccggcg gcggacccgg cggcaaggcc cagcgcgacg gcggcgatca
     4501 gggcggttcg cgtggtcaac cagtgggcca tcgcagggat gctccatggc aagttggatg
     4561 atcctagcaa tcccgggcgg caagtccaaa gaaatcgggg caggtcggcg gcatgacctc
     4621 gggcgcccga ggggccaggc ccggggcgcg gaagggagtg tccgaggatg gggccgcttt
     4681 gaatctgacg atgggagaaa tggggggcgc cgaccggtga ttgccggctc agacccatca
     4741 cgcggcgcgc gatcggccta ccggtcgttc aggatcttcg tccccttggc ggagatgcgt
     4801 tcacagacgg attggacgct ttcctcgccc gcccagataa gctccagttc catgttgatc
     4861 atggcgtaaa gttcgttctg ggccggtgtc tggggacggc tgcggcaatg gtcgagggcc
     4921 gcgatgaagt actcctgatg cgcggggggg cgctggtcca ggtaagccgg gctggcagcg
     4981 agttcccgca aggcgggggt catgatgttg cggtcgatga agatgcgcga accctccgcg
     5041 ccggagagaa attcaaggag cttccatgct tcctcgggat gcctggccct ggttgggatc
     5101 gagaagcagg acccaaagtt ggcgatggcg cggccgttgg gcccgatggg cagcatggcg
     5161 acatcccatt tgaagtccgc ccgcttggtg tattcgggga cggcccagtg gccgttccag
     5221 ccgatggccg cctgcccgtt ctggaacgga tcgatctgcg cggtctgcat ggtttcgctg
     5281 gcgacgacgc cctccttcaa gcgagcgtcc gcgatccatt ggatcgcctt gatcgtgagg
     5341 ggatcgtcga aggtgcagcg ggtcggcttg gcgggatcgt caaccaggtc gccgccgaag
     5401 gaccagatca cactctccca aggcaactga gcggtgccat agacgtcgat gtcgccatcg
     5461 ccgtcggtgt cgcgcgtcag cctgcgggcg atttcgaggt aatcctccca ggtccaatcg
     5521 ggtcccggca cgggcacccc cgccgcctcg aactggtcaa ggttcaggaa gggaatcatg
     5581 atggccgtat cggagggggg gccgtagagc cggccatcga tcgtcagcca ttgcaggggg
     5641 gtcgggtggt aggcctccag gttgatcacg tcgctctggg cgatgagatc ggtcagatcg
     5701 agcagggcgc ccttctcgac aaagccggga tagtaacccg attccatcag gacgaggtcg
     5761 ggtgggttgc ccgaggcggt gagggtgaag aatttggacc agtagccttc ataggggacg
     5821 ttgagcacct tgacctggat ctcggggaac ttcgtcgaaa tggcggcgaa catgtcgccc
     5881 cagatttcct gctcgatcgg cgagcccatg cgcaggtaga tgatgacggg gtcgtccccc
     5941 gcctggcggc agccccccgc ggcggccaga ctcagcgccg tcagcagaag cgagcgccag
     6001 gcgcgggcgc gaaggccgcg gcggaagggg ggttggggtg aggccggcat ggtgcgaatc
     6061 gctttgcgaa gcgccggtca gagctcgtcg accggacgag gttggcccgc ttcttcgagg
     6121 taggcgagga tattggccaa cggggtgatg gcgtcgggcg ccttggcaca ggcgagcaga
     6181 aatccgccgc cgcggcccag ttcgcgtttc atgcggcgga tctcgcggcg gatctcgtcg
     6241 ggcgtgccga agggcatcat ccgctgagcg ccgaggccgc cccagaagcg gatatcccgg
     6301 ccatagcgcc gtttcaggtc atacagatcc atgcactcgg gctgacagct ttccatgcaa
     6361 tcgaggccga cctcgatcag atcggggatg acttcggtgt tgtcgccgca catgtgcatg
     6421 gtggcaaaca gaccgtagtg atggacgcgt tcgtagatcc gcttctagcg cggcttgaag
     6481 agccgccgcc accgatccgc gcccatgatc atgccgcgct ggcaggcgtg gtcgtcggtg
     6541 aagcagatcg aatcgagcgg ggcctgggcc atcaggtcca cgctctgcaa caggtattcg
     6601 gtgatgtggt cgaggagctg ctcggcgaat ttttcgttca gggccagatc gacgaggaaa
     6661 ttttcgaagc cgcgcaaggt ccaggcgcgt tcgaacaagc cgaagacggt ccagcagatc
     6721 cggaagcggt tggccgattg cgcgatctcc cgcggccagc gcggcttgac gtactgttcg
     6781 aagtagggtt ggaggccggg caaacgataa tcgcccatct ccggctcgtg aagcggccaa
     6841 tcctccagat ggctgatgtt ccccatgcgc cacgagcagc cgaggatgtc cttctgcgtt
     6901 ccgtcgggtt gcggcgtcat gccggccagg gtgagcgacc cgtcaaaacc ggtcagcgct
     6961 cccagatagg atacgctgtg ctgctcgcgc cagtgctcgt cgccaaacca ggcggaaagg
     7021 gcctggtcgt gctcatagag catcagatca tcgtagggga caaattccgt ttcctgaaac
     7081 tggaaggcca gtttcacgac atcgcggggc tgcaacgtcg cctcaatggt tttcatgctc
     7141 ggaaccctct tgaaattgct gaagcttgag cgagtatttg tcgcgcaggg aatcagggac
     7201 gttgttggca aggcggtatt ccagattgcc ccggtcaaat ccgggatggt tgagcgccca
     7261 ctcatacagg tccctgcggc ccaggtcggg gagctcgacc ggctggttga tgacgcggcg
     7321 cgtgaggtaa gccgcgaatt cctcctgggg gacggccgcg gcaaacagat cggaatccag
     7381 gatggcgccg agggtcgcat agcgagtgtt gtagccatac tgcaaatcgg cgcgcgccag
     7441 ctttgccagg agctcctgat accaactctg gccgatctgg tcgcccaggc gcaggtccgt
     7501 aaagagggtt ttgaggaatt cctcgtcgga catgatctcg ctggagagat tgatcgagtc
     7561 gcgcaggata tccgcattgg tgagggtatc gtagagccgc gcctggttat tggcttcttg
     7621 ctcggtcgga ttgcgccggc accagacgcg ccgcgcgttc ttgagcgtgt gcatgcggaa
     7681 attgaaatcg cgcagcattt cgtagttgcc gttgggcgtt ccgtcgcttt ggaagagcgc
     7741 ggagtcggtg ttgtcggggg tctggctgta gacctcccag aacaggttga tgaccgagcc
     7801 ccaaccggcg caggccttga gcgcttcgcg attcctggcc cccttctggg gcgtgtagcc
     7861 gcccgggatg ccgaactcac tgatgaagac gggcggcccg tagggccatg cgcccgtcag
     7921 gctcttctcc gcgtttttga ggatgcaatc catgtgatcg tagacgcgct gcgggagatc
     7981 gctggtgtga ttggtcgagt tgtatgcgga attggagacg aggtccatcc ggatgtaggg
     8041 cagcacgcca ttgacgacgc gcataagggt ggggtcctcg atgacgggct gaatcgtaat
     8101 gacttcgaca tagtgataga cttcgacgcc ctcgatctcc ggcagggaat tgcggccgtc
     8161 ctcgaccgcc ttctggcggt tattgagcca gcgccggaga tactccagcc gctcctgcga
     8221 cggctgcacc ttgggatcgg caacctgatc aagcccgctc atcaggacat ggtccgtttc
     8281 ccaattgccg agcagaaaga ccttgccggt cccctggtag gtctccatca ggtatcgcgt
     8341 gaagtcatag agctgctgat attcttcatc gaaggtggcc tgctcccaat cccaggaaaa
     8401 ggtaatgtca ttggtcggcc aggcccagat gacgaagaat ttaaacggca agtcgaagaa
     8461 ttcctgatag gtcggggtgt tctggacgag ccggaccatg gaattcttct tctcgcgggg
     8521 cagttcgggc atatcggggt aaacgtcgcg gtagcccttg ccgcccacgt cgggaccgac
     8581 catcgtcttg aaggcgtcgc agccgatatc gagcatctgg cgggcctgtt cgatgaggcc
     8641 cggttcgtcg gtcagcttct tgtgccattt gaagccctga gccgccccgc ggatcgggaa
     8701 ataggcgaac tccccggagt cggcggaatc ggccgcaaag ccggccagcg gcaggaatgc
     8761 agccacggcc aacaggaaca gagcgttgca gataccccgc aatgtcattg gaaatatcct
     8821 cctggtttca agcaacgttt tcccggaaag acaacgtttc accgcacggg agcgcgccgg
     8881 cccggctact cgtcgcgcag aaaggcgtcg aacagggcca tggcctgctc ggcgctcagt
     8941 ccggcatcga tcaccagccg cgcgcgggtt gtcgcctcct cccccgcctg gaaatcccgc
     9001 ccgaagagcg aaagataaac cgagcggtgg taatcttcgt tctccggcat cgagatggca
     9061 aaacagtcct cgggccggcc catgagaagc gccgccagac ccgtgtcggg gtcgcggcgc
     9121 atcgccaggg gagcggccag tcgcggcatg atgacccacg gcagcgggtg aggctcgagc
     9181 gtccagcgtc cgtccgcgaa gagggccgtc gcggcttcgt cgcgcgggaa ggcctgccac
     9241 ccgccgttgg cggccggggc ttccatgaag ccgcggcggc cgccgtcgtc gggattggcg
     9301 gcgacgcagg ccgaggtaac gggaaatcca ttgaaatacg aggcaaggaa gacttcgaaa
     9361 tggtgcaggt tcttgtgcgg ccggacggtg agacgcagat ccgcgacatt gcgctcgcgc
     9421 cagcgataga cggccgtcat ctccagggga tgctcttcgt cgggccgcca gtcgacgcgg
     9481 acgcttccgt cctgctggac cgccgccgtg ctcggccagt cccaggccgc cgtcccgaag
     9541 cgaacgtcgg gcgtgagcag ccgatagggg gagagcaggc cgaacatccc ggccagttcg
     9601 gtccccgttg cgctgtgggt cagcgggatc agtccgctgg acatgccctc gggatgcagc
     9661 gttcccttga gaaagccggt atcgaaatga aattccggcc cggcggccgt gaacgacaaa
     9721 ccggcggcgg aggcggccgg tagatgctgc tgggaggtcg ggttcatgct cgcgatcctt
     9781 ctggttcgaa attagttcca gcgggaggtt atggggtttc ggttcgagag ggtcagacgg
     9841 tcgccggcgc gggggtcgtt cccacggcgt tcaggtggct acgcaggaat tcctgaacct
     9901 cgatcacctt gcccgtgtgc gccgactcga cggccgcaaa caggagggcg gcgcactgga
     9961 tctcgtcgtc gagatggcag gcc
//
LOCUS       DAOPCO010000145         9973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6498_length_9973_cov_5.900988, whole genome shotgun sequence.
ACCESSION   DAOPCO010000145 DAOPCO010000000
VERSION     DAOPCO010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6498_length_9973_cov_5.900988"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1516
                     /locus_tag="PLS90_10685"
     CDS             176..1516
                     /locus_tag="PLS90_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HPK02909.1"
                     /translation="MTAQLQRLIDDARGIFQAALAGVAPARLLLRRPLCELARRPLAD
                     YARIVVVGVGKAAIGMARSIEEQLGDRLTEGLITVPHGYRDQAGDAALPRRIELTEAG
                     HPVPDEAGRRAAERALAAARGCGPDDLLVVLVSGGGSALWPAFAEDLSLDDARATFNA
                     LLASGASIHEVNAVRKHLSRIGGGRLAAAARPAEVAALLISDVPGDDPGVIASGLTVP
                     DRSTWREALDVVRRYGIEQKLPAAVLCRLERGAVTGADETLKPGDPVFANVTTRLCGS
                     NRDALAAARDEARRRGYSARIADGHVEGEAREVGRRHVRTLRDAAATGPLCLIWGGET
                     TVTLRGDGKGGRNQELALAAALAMEDAPRAIAFLSGGTDGIDGPTAAAGAVVTPATVR
                     AAREAGLAPEEALARNDAWTFFSRLPPGEGSGGHLITGPTQTNVMDVHIGLIGG"
     gene            1553..2974
                     /locus_tag="PLS90_10690"
     CDS             1553..2974
                     /locus_tag="PLS90_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPK02910.1"
                     /translation="MKTPRLRKLILRLATGFAFALVALAIFQWGVGILAVCFYSVFTL
                     AGISWIMTTIWIRPLRVERELSADVVRIGEPVAVTAKIQNRSPWPILWLYAEETLPRA
                     IPREGTWRRLMFIPPGRSFHLSYKLTPNRRGCHPIGPLVLESGDVFGLFKRARIDRRR
                     DWITCLPNYYAIDDFQVGRRRQLGDLRAIRSILDDPSRVQGIREYRRGDPMKIIHWKS
                     TARTGRLCSKVFEPVTDAGATVILDFHQDSWLAIEGAFDRRPPARPMRWGIIPAARRL
                     DKGPGRPATKAEQPPEETAVEVVCTTCRYIADAGWRLGFFCNGRDPLGLPGVSIAQAR
                     ATDSLRAAQQAARRRRDDDRLEPIRIPPQRGPEQFALIHEQLGRIELSDGLRIEDLLL
                     LELPHIDRTQALVVVTGHVTDDFIRGMLRVRASGYRLLVFVVRDNRGHDHAFEHLAPA
                     GIELYRMDEDWRLREIAIGRQFA"
     gene            2943..4670
                     /locus_tag="PLS90_10695"
     CDS             2943..4670
                     /locus_tag="PLS90_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPK02911.1"
                     /translation="MRSPSVDNSPERGLSNLLTAILIPVCVWGMLATLLLHLLDVRAV
                     FIHDDAWRMRQAVLAFAAGVILIQMLSREQGPRTAGAYGWLLGLSMTAFAVYQAFAYR
                     IAIPWPLVALVNLTLFGLLWWVGQRVMTACTVHDQAAAHAAETGVFSRAGSAGPEAEV
                     PPDAAPDALPPGEDLGVSELYAGVRRERERKRLERNAAEDEAALRERMNVPHPGRVIF
                     RFSLFAIPAFGAGFLLLGGQHEIFPRLRVGILLFLYLYCSLLLLCLAGLSQMRAYFNA
                     RGVRMPDPLALTWIGLGLSLVTLALALAFFLPQPVTAPQRFVRDQMVGVYRGFEGKRG
                     APSQLAVSTPEDQAPPRARKFHRDELNQMLKERYEPIDKLGDEYISELARTTGAEPEY
                     QNAMKLRAAMQETYHQVGQFAMKAIFALAILGGVVVLVVLAWVFLGRLSEGLRRLRLA
                     RRPRRPRRPRRKAGTPVPLERFGRFANPFTRGYDPGDGNALVRYLWQATLAYCADAGA
                     PCPPDLTPLEFVEQNPAPLAGFERNAMFIADLVSYSEFSGQPIPAETLPRLREYWSAL
                     ARHAHARGA"
     gene            complement(4693..5907)
                     /locus_tag="PLS90_10700"
     CDS             complement(4693..5907)
                     /locus_tag="PLS90_10700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPK02912.1"
                     /translation="MVLALADPPIADLEPNDDVFAMRVLVVEDDPFTLTILNKRLVKG
                     GYTVETATNGREALDCVARFKPHLILSDWMMPEMDGVELCTRVRELPDGPSVYFILLT
                     AKDKNDDKVSALDTGADEYLVKPCDSAELMARLRAAERILRLRQQLASRNRELRQAMR
                     RINQELQATSQIQRSLLPQELPTVEGYHFAAHYQPSTECSGDFYDLIPRPDGRLSVVI
                     GDVSGHGTPAMVAMAMMHLLLQVEAPAAEDPARLLHLINNRMFQSLPTGQYCTMFFGI
                     LDPKTGELAYSSAGHNAPLHVCPGERTARFLPDCEGFPIKLVAPDLSYETHHHQLAPG
                     QSLVLYTDGLPEAFNRGDEAYGYDRLRDVVETLGHNDPELIMDAMLMDMHLFAGDRPL
                     DDDLSLLILSRE"
     gene            complement(5995..6426)
                     /locus_tag="PLS90_10705"
     CDS             complement(5995..6426)
                     /locus_tag="PLS90_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02913.1"
                     /translation="MALLLVVEDDPLTAKVVQKVLTRMGGHEVKLAANPAGALQALEE
                     HPIELIVMDVTLIDATLNGEPVDGVKLTRHIRRDSRWRQVPVILATAHAMSGDRERYL
                     AEAQADDYIAKPIVDHHAFCAQIAALLARKLEGAGRMPVGP"
     gene            complement(6773..7351)
                     /locus_tag="PLS90_10710"
     CDS             complement(6773..7351)
                     /locus_tag="PLS90_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPK02914.1"
                     /translation="MPETPAVHRLILVLGLCCLFAAGSTGVRAGAPAHGTAPEPTAGA
                     AADEAEAAASGETGHGKAAEPQRPAFRLGPELKGFYDYRVLRARDRLEPYRYPLEGIV
                     VQDSDGLRKQKIELGVVIEFGGESGMAELQSLEASVRQEIERLLRDIRFGELIHPEGK
                     SRLKELIVRTVDRQLKTARVRQVYLTEFRVNL"
     gene            7634..7819
                     /locus_tag="PLS90_10715"
     CDS             7634..7819
                     /locus_tag="PLS90_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02915.1"
                     /translation="MNRAKHIQQNVVKRFTEQQMPEVATRGLFADPLRSHPDHLRQFL
                     TMAENRRRQILERMLME"
     gene            7827..8543
                     /locus_tag="PLS90_10720"
     CDS             7827..8543
                     /locus_tag="PLS90_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02916.1"
                     /translation="MGAEIQILVIDDDPSLLAVLETGLQLEPGYHIRAEPRAADGLAA
                     LRAQPFDLVVTDYSLDDPQINGLTILQTAHALAPAPLVVIITAFASLELTLKSIQLGA
                     HDFLTKPFQLEELRLVVRNAARLIELRRENERLHEQVGALDRAMRRIERAHAELVHRL
                     DQLNDDATGNSGTDPGLTPASFQALELRRRRMRDQIAVYARMGHEIGEQLLAERSRLG
                     VQFTTGQPDESEQVACSPGD"
     gene            8673..>9973
                     /locus_tag="PLS90_10725"
     CDS             8673..>9973
                     /locus_tag="PLS90_10725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK02917.1"
                     /translation="MKMPAIQTVLQGNILVVDDDPDCRLIMQEILRHEGHTCTCLASG
                     AEARDAFDAGQFDLMITDLYLQDASCSGLELIDYVTRRDETIPVILVTGYPSIPRAVD
                     AMRRGAVDFIAKPFDHDFLLHQVHKALQERRLRRENQRLLAEVNKAAVIEKLNRELDR
                     RVDELTRLFAISEGLNQFMPTDGLFHRIAYLAGEVTGAQRVSVMVFDRSRQYLKIRSA
                     IGLPHEIVEKTVQPVGRGVAGHVAATGKPLHVIDRMIDLCLRVEDAREVAYRSGSWIA
                     VPLRIGQQVFGVINVTDKVDGSDFTESDRHIMESLAEKAGGKLENQALYEGIYSNLVD
                     TLTSLVTTIEAKDPYTREHSQRVTDYAITLARAMGLSQDQVEMLNFAGMLHDIGKIGV
                     RDGILTKAGRLSDEEYCQIKLHPEIGERIVEPLGLAPMEKA"
BASE COUNT         1568 a         3602 c         3152 g         1651 t
ORIGIN      
        1 cggaatcggg atcaatatcg gaatcaaaca accccgcaac cgcacggaac tccgcggcga
       61 ctccattttg ccaaccgcat gagcggctgg ttttctgatc cacggcgaat gtcggaatgg
      121 ttcgaataga tcactagaac gggcgaagcc ggacgagttt tcgtggaagg gccaaatgac
      181 cgctcaactc cagcgactga ttgacgacgc gcgcggcatc ttccaggcgg cgctggcggg
      241 cgtcgcgccc gcgcgtcttc tgctccgccg gccgttgtgc gaactggccc ggcgcccgct
      301 cgccgactac gcccggatcg tcgtcgtcgg cgtgggcaag gccgccatcg ggatggcgcg
      361 gtcgatcgag gagcaattgg gcgaccggct gaccgaaggg ttgatcactg ttccgcacgg
      421 ttaccgggat caggcgggcg acgcggcgct cccgcgccgg atcgaactga ccgaggcggg
      481 gcatccggtg cccgacgaag ccggtcggcg cgccgccgag cgcgccctcg cggcggcgcg
      541 cggctgcggc cccgacgatc tgctcgtcgt cctcgtttcg ggcggcgggt cggcgctctg
      601 gccggccttc gccgaggacc tgtcgctcga cgacgcgcgg gcgacgttca acgcgctcct
      661 ggccagcggc gcgagcattc acgaggtcaa cgcggtgcgc aagcatctgt cgcggatcgg
      721 cggcggtcgc cttgcggcgg cggcccggcc ggccgaggtt gccgcactgc tgatctccga
      781 cgtgccgggc gacgacccgg gggttatcgc cagcgggctg acggtccccg accgctcgac
      841 gtggcgcgag gcgctcgacg tcgtccgccg ctacgggatc gagcaaaagc tccccgccgc
      901 cgtcctgtgc cggcttgagc ggggcgccgt caccggcgcc gatgagacgc tcaagccggg
      961 cgacccggtg ttcgcgaacg tcacgacgcg cctctgcggc tcgaatcgcg acgcgctcgc
     1021 ggcggcgcgc gacgaagccc gccggcgcgg ttactccgcg cgcatcgccg acggccatgt
     1081 ggagggtgag gcgcgcgagg tcggccgccg ccatgtccgg acgctgcggg acgcggcggc
     1141 gaccgggccg ctctgcctga tctggggcgg cgagacgacc gtcacgctgc gcggcgacgg
     1201 caagggcggg cgcaatcagg aactggcgct cgccgccgcg ctggcgatgg aggacgcacc
     1261 ccgcgccatc gcgttcctca gcggcggcac cgacgggatc gacgggccga ccgcggccgc
     1321 cggcgccgtc gtgacccccg ccaccgtccg cgcggcgcgc gaggccggcc tcgcgcccga
     1381 ggaggcgctc gcccgcaacg acgcctggac tttcttcagc cgcctgcccc cgggcgaggg
     1441 gagcggcggt catctcatca ccggccccac gcagacgaac gtcatggatg tccacatcgg
     1501 cctgatcgga ggataactcc ctcacgccgc cgacgatccg ggatcgacca gaatgaaaac
     1561 cccccgcctg cgaaaactca tcctccggct cgcgacgggt ttcgcctttg ccctcgtcgc
     1621 gctcgccatt tttcaatggg gcgtcggcat ccttgccgtt tgtttctact ccgtcttcac
     1681 cctggccggc atttcgtgga tcatgacgac gatctggatc cggccgctgc gcgtcgagcg
     1741 cgaattgtcg gccgatgtcg tccgcatcgg cgaaccggtg gccgtgacgg cgaaaatcca
     1801 gaaccgctcg ccctggccga tcctctggct ctacgccgag gaaacgctcc cccgcgcaat
     1861 tccgcgcgaa ggaacctggc gccggctgat gttcatcccg ccggggcgga gcttccacct
     1921 cagctacaag ttgacgccca accggcgcgg ctgccacccg atcggcccgc tcgtgctgga
     1981 gagcggcgac gttttcggcc tgttcaaacg cgcccgcatc gaccgccgcc gcgactggat
     2041 cacctgcctc cccaactact acgcgatcga tgattttcag gtgggccgcc gccgccaact
     2101 cggcgacctg cgcgcaatcc gcagcatcct ggacgaccct tcgcgcgtgc agggcatccg
     2161 cgaataccgc cgcggcgatc cgatgaagat catccactgg aaatcgacgg cgcgcaccgg
     2221 ccggctctgc agcaaggtct tcgagccggt gaccgacgcc ggcgcgaccg tcatcctcga
     2281 tttccaccag gattcctggc ttgccatcga aggcgcgttc gaccgccgcc cgcccgcccg
     2341 gccgatgcgc tggggcatca tccccgccgc ccgtcggctc gacaagggcc ccggccgccc
     2401 cgccaccaag gccgaacagc cccccgagga gacggccgtc gaagtcgtct gcaccacctg
     2461 ccgctacatc gccgacgccg gctggcggct gggtttcttc tgcaacggcc gcgatccgct
     2521 cgggctgccg ggcgtcagca tcgcccaggc gcgggcgacc gattcgctgc gcgcggcgca
     2581 acaggccgcc cgccggcggc gcgacgacga ccgcctcgag ccgatccgca tcccgcccca
     2641 gcgcgggccg gagcagtttg ccctgatcca tgagcagctc ggccgcatcg agctctccga
     2701 cggcctgcgc atcgaagacc tgctcctgct cgaactgccc cacatcgacc gcacccaggc
     2761 gctcgtcgtg gtcaccggtc atgtcaccga cgacttcatc cgcggcatgc tgcgcgtgcg
     2821 cgcctcgggc taccggctgc tggtgttcgt cgtgcgcgac aaccgcggcc acgatcatgc
     2881 ctttgaacac ctcgcccccg cgggcatcga actgtatcgc atggacgaag actggagact
     2941 gcgtgagatc gccatcggtc gacaattcgc ctgagcgcgg gctctccaac ctgctgacgg
     3001 ccatcctgat cccggtctgc gtctggggga tgctcgccac gctgctgctg cacctgctcg
     3061 acgtgcgcgc ggtgttcatc cacgatgacg cctggcgcat gcggcaggcg gtgctcgcct
     3121 tcgccgccgg cgtgatcctg attcagatgc tctcgcgcga gcagggcccg cgcacggccg
     3181 gggcctatgg ctggttgctc ggcctgtcga tgaccgcctt cgccgtctat caggccttcg
     3241 cttatcggat tgcgatcccc tggccgctcg tggcgctggt caacctgacg ctgttcggcc
     3301 tgctgtggtg ggtcggccag cgcgtgatga cggcttgcac ggtgcacgac caggcggcgg
     3361 cgcacgcggc ggagacgggc gtcttctcgc gcgccggttc ggccggaccc gaggccgaag
     3421 tccctccgga cgctgctccc gatgccctgc cgcccggcga ggacctcggc gtctcggagc
     3481 tgtatgccgg cgtccgtcgc gagcgcgaac gcaaacgcct cgagcgcaac gcggcggaag
     3541 acgaggcggc gctgcgcgag cgcatgaacg tgccgcaccc cggccgcgtc atcttccggt
     3601 tttcgctctt cgccatcccc gcgttcggcg ccggattcct gctgcttggc ggtcagcacg
     3661 aaatcttccc ccggctgcgc gtcggcatcc tcctcttcct ctacctctac tgctcgctgc
     3721 tgctgctttg cctggcgggg ctgagtcaga tgcgcgccta cttcaacgcc cgcggcgtgc
     3781 ggatgcccga tccgctggcg ctgacctgga tcggcctggg tctgagcctc gtcacgctcg
     3841 cactcgcact ggccttcttc ctgccgcagc cggtcaccgc gccgcagcgc ttcgtccgcg
     3901 accagatggt gggggtctat cgcggtttcg agggcaaacg cggcgcgccc agccagctcg
     3961 ccgtttcgac gccggaagac caggccccgc cccgcgctcg caagttccac cgcgatgaac
     4021 tgaaccagat gctgaaggag cgttacgagc cgatcgacaa actgggcgat gagtatattt
     4081 ccgaactggc gcgcaccacc ggcgcggagc ccgaatacca gaacgcgatg aaattgcgcg
     4141 ccgcgatgca ggagacctac catcaggtgg gacagttcgc gatgaaggcg atcttcgcgc
     4201 tggcgatcct cggcggcgtc gtggtcctcg tcgtgctggc gtgggtcttc ctcggccggc
     4261 tgagcgaggg gctgcgccgg ttgcggctgg cccgccgccc gcggcgacca cgccgtccgc
     4321 gtcggaaagc cggcacgccc gtgccgctgg agcgcttcgg ccggttcgcc aatcccttca
     4381 cgcggggtta cgacccgggc gacggcaatg ccctcgtgcg ttacctgtgg caggcgacgc
     4441 tggcctactg cgccgatgcc ggcgcgccgt gtccgcctga cctcacgccg ctggagttcg
     4501 tggaacagaa tcccgcgccg ctcgccggtt ttgagcgcaa tgcgatgttt atcgccgatc
     4561 tggtcagtta cagcgaattc tccggccagc cgatccccgc cgagacgctg ccgcggctgc
     4621 gcgaatactg gtccgcgctc gcccgccacg cccacgcccg cggcgcctga acagaaggat
     4681 ggaatccgcg cttcattcgc gcgacaggat cagcagtgaa agatcgtcgt ccagcggacg
     4741 gtcgccggcg aacaggtgca tgtccatcag catcgcgtcc atgatcagtt ccgggtcgtt
     4801 gtgccccagc gtctcgacca catcgcgcag ccggtcgtac ccgtaggctt cgtcgccgcg
     4861 attgaacgcc tcgggcaacc cgtcggtata cagcaccagg ctctgcccgg gggcgagctg
     4921 gtggtgatgc gtctcatacg atagatcggg cgcgaccagc ttgatcggaa agccctcgca
     4981 atcggggagg aagcgcgccg tgcgttcccc cgggcagacg tgcagcggcg cgttgtgccc
     5041 cgccgagctg taagccagct cgcctgtctt cgggtccagg atgccgaaaa acattgtgca
     5101 atactggccg gtgggcaggc tctggaacat ccggttgttg atcagatgca gcagccgggc
     5161 cggatcctcg gccgcggggg cttccacctg caggagcagg tgcatcatcg ccatcgccac
     5221 catcgccggc gtgccgtggc cgctcacatc gccgatgaca acgctcagcc ggccgtcggg
     5281 gcgcgggatc aggtcgtaaa aatcgccgct gcactccgtc gagggctgat agtgcgccgc
     5341 aaagtgatag ccctcgaccg tcggcagctc ctgcggcagc aggctgcgct gaatctggct
     5401 ggtcgcctgc agctcctgat tgatccgccg catcgcctgg cgcagctcgc ggttgcggct
     5461 ggccaattgc tggcgcaggc gcaggatgcg ttcggccgcg cgcagccgcg ccatcagttc
     5521 cgcgctgtcg cacggcttga ccagatactc gtccgcgccg gtgtccagcg ccgacacctt
     5581 gtcgtcgttc ttgtccttcg ccgtcagcag gatgaagtag accgacgggc cgtcgggcag
     5641 ctcgcgcacc cgcgtgcaga gctccacccc gtccatctcc ggcatcatcc agtccgacag
     5701 gatcaggtgc ggcttgaacc gcgcgacgca gtcgagcgcc tcccgcccgt tggtcgccgt
     5761 ctcgaccgtg tagcccccct tgaccagccg cttgttcaga atcgtcagcg tgaacgggtc
     5821 gtcctcgacg accagcaccc gcatcgcaaa gacgtcgtca ttgggctcca ggtcggcaat
     5881 cgggggatcg gctagggcaa gaaccatgag ttatcggatt cgcgcgaagt gttaacccgg
     5941 agtcatccga gcgacattgg cctggcggcg accgggcgtc ggccgccgcc gaactcacgg
     6001 gccgaccggc atcctgcccg cgccctcgag cttgcgcgcg agcagggcgg cgatctgagc
     6061 gcagaaggca tggtggtcga cgatcggctt ggcgatatag tcatcggcct gggcctcggc
     6121 caggtagcgc tcgcgatcgc cgctcatcgc atgggccgtc gccaggatca ccggcacctg
     6181 ccgccagcgc gagtcccggc gaatgtgccg cgtcagcttg acgccgtcga ccggttcgcc
     6241 gttgagcgtc gcgtcgatca gcgtcacgtc catcacgatc agttcgatcg ggtgttcctc
     6301 cagcgcctgc agcgcccccg ccgggttggc ggcgagtttc acctcgtgtc cgcccatccg
     6361 ggtcagcacc ttctgcacga ctttcgccgt cagcggatcg tcttcaacta cgagcaacaa
     6421 ggccatgcgg tagtatcctt gggtccggcc cggggggagc ggtcgtcgtc cgtcggcgcc
     6481 ggcagggtct cagcttcgcc atcggcaatt gaacttatac gtcttttcgg tcgccccgac
     6541 cctccgctta agcgcccccg cccctcatcc tcgctcaaaa tcgttcctcg tccctcactc
     6601 gaaaatctgt ctttcgccgg gatcgcttgg atcggttctg ccacctctac aggactccgt
     6661 cgctttggtt gtgaatcgca gattcatgag caactcgaga tcgcccccaa cggccttctc
     6721 gagaattcgc ccgcggctct cgaaaaccgc cccttcgccc cccgtctctc gctcacagat
     6781 tgacgcggaa ctccgtcaga tagacctggc ggacccgcgc cgtcttgagc tgccggtcga
     6841 ccgtccgcac gatcagctcc ttcagccggc tctttccctc gggatggatc agttcaccga
     6901 agcggatgtc gcgcagcagc cgttcgatct cctgccgcac cgacgcttcc aggctctgca
     6961 gttcggccat cccgctctcg ccgccaaatt caatgaccac ccccagttcg atcttctgct
     7021 tgcgcaggcc gtcgctgtcc tgaaccacga tcccctcgag cgggtagcga tacggttcca
     7081 gccgatcgcg cgcgcgcaag acccgatagt cgtagaaccc cttcagttcc gggccgaggc
     7141 gaaacgccgg ccgttgcggc tcggccgcct tgccgtgccc cgtttcgccg ctcgccgcgg
     7201 cctccgcctc atcggccgcc gccccggcgg tgggctccgg cgccgtccca tgcgcgggcg
     7261 cgcccgcgcg cactccggtt gaaccggccg cgaacaggca gcacagcccc agcacgagga
     7321 tcaggcgatg gaccgcgggc gtttccggca tgcgcgcgac tcccttccat ctcgcttatc
     7381 ggcgcattgc cctccggact tgcccgcttt gcgtaggtgc tacgaaaatg catcgacgcc
     7441 gagcgtccat cccgtccatt gcgtccagct tgtccatcct ctcctgctct tccccaaaac
     7501 ccctcagtgt ccgctaacgg gaacgggggt cgacccggcc gctggctgaa tcgataagta
     7561 aaccgaacgg cagctatccc cgtcgtcggc gggggcgtcg gatgattgga tcatcaagtc
     7621 ggagcaagcg ggtatgaacc gggccaaaca cattcaacag aacgtcgtga aacgattcac
     7681 cgaacaacag atgcccgagg tggcgacgcg cgggctcttt gccgatccct tgcgctcgca
     7741 ccccgaccat ctgcgccagt tcctgacgat ggccgagaac cgccggcgtc agatcctcga
     7801 gcggatgctg atggagtaac ccggtcttgg gcgccgagat tcagattctc gtgatcgacg
     7861 acgatccctc gctgctggcc gtcctcgaga ccggcctgca attggagccg ggctaccaca
     7921 tccgcgccga gccgcgcgcg gccgacggcc tggcggcgct tcgcgcccag ccgtttgacc
     7981 tcgtcgtcac cgactattcg ctcgatgacc cccagatcaa cggcctgacg attctgcaga
     8041 cggcccatgc gctggcgccc gccccgctgg tcgtgatcat caccgctttc gcctcgctgg
     8101 agttgaccct caaatcgatc cagctcgggg cgcatgattt cctgaccaag ccgttccagc
     8161 tcgaggaatt gcgcctggtt gtgcgcaacg cggcccgctt gatcgaactg cgccgcgaaa
     8221 acgagcgcct gcacgaacag gtcggcgccc tcgaccgcgc gatgcggcga atcgaacgcg
     8281 cccacgccga actggtgcac cggctggatc aactcaacga cgacgcgacg ggcaactccg
     8341 gaaccgaccc cggcctgacc cccgcctcgt tccaggcgct ggagctgcgc cgtcgccgga
     8401 tgcgcgatca gatcgccgtc tacgcccgca tgggccacga aatcggcgag caactgctcg
     8461 ccgagcgttc gcgtctcggg gttcagttca cgaccggaca acccgatgaa tctgaacagg
     8521 ttgcctgttc gccaggagac tgagccgacc gtccggaagc aacacgatcc cgggccaagg
     8581 tgtcgcgcaa gcccgggttc gcacccgacg ccagggaagg cgtcaaccac ggcatgaccc
     8641 ggccgcccca cggaaggaag accacgcgac tcatgaagat gccggccatc cagaccgttc
     8701 tgcaggggaa catcctggtc gtcgacgacg acccggactg ccgcctgatc atgcaggaaa
     8761 tcctgcgcca cgaggggcat acgtgcacct gcctggcgtc gggcgccgag gcgcgcgacg
     8821 ccttcgatgc cggccagttc gatctgatga tcaccgacct ctatctccag gatgcctcct
     8881 gctcgggcct ggagctgatc gactatgtca cgcgccgcga tgaaacgatt ccggtgatcc
     8941 tcgtcaccgg ctacccctcg attccgcgcg ccgtcgacgc gatgcgccgc ggcgccgtcg
     9001 acttcatcgc caagccgttc gaccacgatt tcctcctgca ccaggtgcac aaggcgctcc
     9061 aggaacggcg gctgcgccgc gaaaaccagc gcctgctggc cgaggtcaac aaggcggcgg
     9121 tgatcgagaa actcaaccgc gagctcgacc ggcgcgtcga tgagctgacc cgcctgttcg
     9181 ccatcagcga gggcctcaac cagttcatgc cgaccgacgg cctgttccac cgcatcgcct
     9241 acctggcggg cgaagtgacc ggcgcccagc gcgtcagcgt gatggtcttc gaccgctcgc
     9301 gccagtacct caagatccgt tcggcgatcg gtctgcccca cgagatcgtc gagaaaaccg
     9361 tccaaccggt cgggcgcggc gtcgccggcc atgtcgccgc caccggcaag ccgctgcacg
     9421 tcatcgaccg gatgatcgac ctctgcctcc gcgtcgagga cgcccgcgag gtcgcctacc
     9481 gcagcggctc ctggatcgcc gttccgctgc gcatcggcca gcaggtcttc ggcgtgatca
     9541 acgtcaccga caaggtcgac ggttcggact tcaccgagtc cgaccgccat atcatggagt
     9601 cgctcgccga aaaggccggc ggcaaactcg aaaaccaggc tctctacgag ggcatctact
     9661 ccaatctggt cgatacgctc acgtcgctcg tcacgacgat cgaggccaag gatccctaca
     9721 cccgcgaaca cagccagcgc gtcaccgact acgcgatcac gctggcccgc gcgatgggac
     9781 tcagccagga ccaggtcgag atgctcaact tcgccggcat gctgcacgac atcggcaaga
     9841 tcggcgtccg cgacggcatc ctgaccaagg ccgggcgcct cagcgacgag gaatactgcc
     9901 agatcaagct gcatcccgag atcggcgagc ggatcgtcga gccgcttggc ctggcgccga
     9961 tggaaaaggc cat
//
LOCUS       DAOPCO010000146         9855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6591_length_9855_cov_5.176735, whole genome shotgun sequence.
ACCESSION   DAOPCO010000146 DAOPCO010000000
VERSION     DAOPCO010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6591_length_9855_cov_5.176735"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PLS90_10730"
     CDS             <1..205
                     /locus_tag="PLS90_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02918.1"
                     /translation="SPDSAVALAHGDTVQVVQTLFLFMDRRQAEGAGAEEIAIDRATV
                     AAEAARLLKENPELHTLRRSRRA"
     gene            complement(243..1262)
                     /locus_tag="PLS90_10735"
     CDS             complement(243..1262)
                     /locus_tag="PLS90_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPK02919.1"
                     /translation="MALIRQRLGSVRPQCVDAELAAGRSEILQPVAPANYRVLTALAT
                     NPDNYFVLSCGGGSAPALAGNAALTAILEELDLRRHVREIWGTSAGAIVGGGWATGTP
                     APRMLELLIDMNRSGIVDCSRWDLFGWPMLQTLLRRRLPEGFIPGRHFRDYTQRGLRV
                     ATFADCEIPFRAIACRDDGSARKVVFRDGPLINAIMASICLPGIMRPVPDWSGGEFGY
                     FDGGVLEKTPLASIIEDHARQLRRQRLVVLCSHFNYCDKTSPPRGFMQRFLASNNLME
                     GLVWDTQLERARQGAGKFLVVSPEIRHGAYFDFSRIEEYYLAARRDFTHQLSNAGMAA
                     RFDAR"
     gene            complement(1262..2965)
                     /locus_tag="PLS90_10740"
     CDS             complement(1262..2965)
                     /locus_tag="PLS90_10740"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPK02920.1"
                     /translation="MAVRTKLIILTMRRSARRFETATRDPAEAQQRKLLWLMDKNKDT
                     EYGRRHGFASVRTFTDFQRQIPVASYEDIQEDMQRVFEGAENVFTAEKPIMFAQTSGT
                     TGTPKFIPVTPTDYGRDHRDVMRTWLYHSQCAHPDILDAKVVSLVSPAVEGHAPSGVP
                     FGSTSGHIYQNMPAIIRKTYSIPYPVFEISDYSAKYYVIMRLAVADDVRFLNTANPSS
                     ILKMCEKGNEFSEDILRDIRDGTLSPTLELEPEIRKEVEAMLRPNPERARELERARSA
                     RDGRLAPADYWPRLSLIACWKGGTVGHYLAKFDDWFNPDGRRPIPVRDWGYLASEMRG
                     SIPLSDEGSAGPLTVATNLFEFVSVDQFDANPDDPAAWTFLTVDQIQDQGEYYVFVTT
                     SGGLYRYDINDVVQVQGYYNKTPQVVFLRKGRGMTNLTGEKISVNQVIQTFETVSRES
                     GLTIAHFRVEADQDRERYLFRIEPIAAPAREQARQVLRALDARLQEINLEYKAKRASQ
                     RLKDPVLHVMREGWYERERRRQTSAGRRAFQAKTVVLTPIKAPTQEIRPEIGDIVEFN
                     D"
     gene            complement(2971..3966)
                     /locus_tag="PLS90_10745"
     CDS             complement(2971..3966)
                     /locus_tag="PLS90_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK02921.1"
                     /translation="MSPRPIRLVLTMLPAALLAGCAGVPQRAASRTPVDFYDEGDSAI
                     QPLVEDFAAGDGVRLRFVHYPPERANGVRLVYLNGIESHAGWFEAPARELARSGYELY
                     LLDRRGSGLNQSGPGLRAGHVDHADTLIGDIHTFRAALPPDGPLGLVALSWGGKLAVG
                     HALRHPADFDFLVLVTPGLVPRVDVTPAQKIGILFAQPFRPTALFQTPIEPWMFTRNP
                     HFLARIERDPLRLREATARFLWESRRLDRQIESEIGEFRTPVLLLLAGDDVIIDNAAT
                     AALLARGQSPLITIRRYPGLPHSLQFEIPAAIARAMDEWITGLDAARPGSSRGSG"
     gene            complement(3963..4730)
                     /locus_tag="PLS90_10750"
     CDS             complement(3963..4730)
                     /locus_tag="PLS90_10750"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPK02922.1"
                     /translation="MTGRRRIISLHSYRGGTGKSTLAANFAWFAAARGDRVAVLDCDL
                     VSPGIHILFGLERDQIQLTLVDFLWGKCGLEETAYDVTERLRPPGDGKCWLIPASLTA
                     QAITRVLEEGYDVQRLNSHFDQLLEYLDLDWLLIDTHPGLNRETLLTTAISDQLILIL
                     RPDQQDYYGTAVINELAAKLEIRRIHLILNKVYSQADTAALTTKVTEAFDHPVIGVLP
                     LSEDLARLQSREIIARAQPEHAVSRTIHDMAETIFSS"
     gene            complement(4727..6934)
                     /locus_tag="PLS90_10755"
     CDS             complement(4727..6934)
                     /locus_tag="PLS90_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HPK02923.1"
                     /translation="MIDTPGNRPDQAGFLERLLPRRRFALLRQNKKLVFFIFCMGSFL
                     ALLGFATHNAIETALKQVLAEYLNAVLEADVAALQMWFEEEEQEVRRWAAHPAVVERV
                     AALSMLATDRTTTSDTLLADSAQQELRRFLSLIHEDENHVGFQVYDRTGLILAAHDDP
                     PVGRRLNGEGMALLGKVFEKGPFVSHPLHTGSHVSGQVVDVNRPVMLSLAAVETDDEQ
                     PIGAIALLINPEQEFTRIISIARVGATGNTYAFNRNGVILSDSRFEEQLKEIGLLPNT
                     NQSQSILNLEIRDPGGDLTRGFQPDGLPAARPLTAMAASATSGQNGYNLEGYRDFRGV
                     PVLGAWRWLPDYDLGLAAEVSIQEALDAIRPLTYSFRILFWLLVLVMLGILVTNAIIS
                     RLRRDIRASQRIDKYTLERKIGGGGMGEVYLARHALLRRATAIKLLHTDQSDTPTLER
                     FEREVQLTSQLAHPNTIEIYDHGVTDEGIFYYVMEYLHGITLARLLELQGEVPPDRVI
                     HILRQVCGSLREAHAHQLIHRDIKPLNIMLCDLGGEPDFVKVLDFGLVKELAIDGEPA
                     VTSTNVINGTPLYIAPERLRDPTRVDTRTDIYSLGVVAFNLLTAQELFEGATAMELSQ
                     ATLASPVPRPSSVAQQPIPAQLDELVVRCLAKNPDDRPQRVDQIIDELEQLRLEHPWS
                     RAAAVEWWQRHADQIDPAPGDPETQADPREVDTGARTLPVMQQGRPAVGVERP"
     gene            complement(7022..8179)
                     /locus_tag="PLS90_10760"
     CDS             complement(7022..8179)
                     /locus_tag="PLS90_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HPK02924.1"
                     /translation="MRIRHFEVFQTALPFRVRFRHAQASRSHSASIFVRCELADGAVG
                     HGEGLPREYVTGESWEGCFQNLTQRILPPLLDCDFATIGEVEAFCGGLYERLVSGAAG
                     IAERPGAACCAAELALLDAYGRHFGATSFAHSGAGSDPVYSGVISSSGIVAAAVTAWR
                     CRRAGVPAVKLKAGTPRDGAVLRVARRILGPAVSLRMDANMGWEFDEARRAIMMARRH
                     AVHYVEQPLARGRHELCRRLADETGARLIADEDLCDLRDAETLVEAGACQVFNIRVSK
                     CGGFFNSLRLGHFARRHGLEVQVGCQVGESALLSAVGLAVARRLAPVVFMEGSFGTRL
                     LVDDLSGWASGHGFAFGPGGRAPRYDPESGIGIALNEAALRRYSVRSCRIG"
     gene            8254..9141
                     /locus_tag="PLS90_10765"
     CDS             8254..9141
                     /locus_tag="PLS90_10765"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK02925.1"
                     /translation="METTIISTDKIDVALGHQELMAVRTSDGVVIPMRYWPGREQRGA
                     VLFVHGMLSHSAWFIPTGDWLNQLGFTCVALDRRGSGRSEEARGHMLSYHQLVDEIEG
                     AVGWLESEHPGAPIHLAGQCYGALPVLRFAAAHPERCRSLTLLSPSIRVHATLTLREK
                     LLVALLAKVRPTHPMRVPLKPEHFTDLPEHLEFIRGDALRLQYVTTAFYLQTLLMMLA
                     VRGSADRIVTPTFIALAGRDEVCDNAAAERFFSQLAAEDKRLTTYPGSMHSLEYGRDR
                     LAFLADYRHWLADHDQIVS"
     gene            complement(9339..>9855)
                     /locus_tag="PLS90_10770"
     CDS             complement(9339..>9855)
                     /locus_tag="PLS90_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02926.1"
                     /translation="TLLRRRDAGYANAAQGEVWVEGQACLREMDAPSPTHDLDAVYDT
                     QRERLRTTREALMLPREAVGVVIARDDRILGLELMDHPSSFRTTWDKLADGYYVEALR
                     RRRPPEEERPAELSELSLRWFLGRVADSLTVRWNQTGAGIGLALDDPRITGSGVWHDG
                     RLCQLCALAVE"
BASE COUNT         1720 a         3235 c         3236 g         1664 t
ORIGIN      
        1 gagtcccgac agcgccgtcg ccctggccca cggcgatacg gtgcaggtcg tccagacgct
       61 gtttcttttc atggaccggc gccaggcgga gggggccggc gccgaggaga tcgccatcga
      121 ccgcgccacc gtggcggcgg aggccgcgcg cctgctgaag gagaaccctg aattgcacac
      181 cctgcgccgg tcgcgacggg cgtaagcggc ggggatcgac ccgcggccgc ggcgagtgcg
      241 gttcagcggg cgtcgaaacg cgccgccatt ccggcgttgg aaagctggtg cgtgaaatcg
      301 cgccgcgcgg ccagataata ctcctcgatc cgcgagaagt caaaataggc gccatggcgg
      361 atctccgggc tgacgacaag aaacttgccg gcgccctgac gcgcccgctc cagttgcgtg
      421 tcccagacca atccttccat gaggttgttc gaagccagga agcgctgcat gaatccgcgt
      481 gggggcgagg tcttgtcgca atagttgaaa tgcgagcaga gcacgaccag gcgctggcgg
      541 cgaagctggc gcgcatggtc ctcgatgatc gacgccagtg gggttttctc cagcacgccg
      601 ccgtcgaagt agccgaactc cccgccggac cagtcgggga cgggccgcat gatccccggc
      661 aggcagattg atgccatgat ggcgttgatc agcggcccgt cgcggaagac gaccttgcgg
      721 gcggacccgt cgtcgcggca ggcgatggcg cgaaatggga tctcgcagtc cgcgaacgtg
      781 gcgacccgca ggccgcgttg cgtgtagtcc cggaaatggc ggccggggat gaacccctcc
      841 ggcaggcggc ggcgcagcag cgtctggagc atcggccagc cgaacagatc ccagcgcgag
      901 caatcgacga tgccggaccg gttcatgtcg atcagcaatt cgagcatgcg cggcgcgggg
      961 gttcccgtgg cccagccgcc gccgacgatc gcgccggcgg aagtccccca gatttcgcgc
     1021 acgtggcggc gcaagtccag ttcctcgaga atcgccgtga gggccgcatt gcccgccagc
     1081 gcgggggcgc tgccgccgcc gcaactgagg acgaaatagt tgtcgggatt cgtcgccaga
     1141 gccgtcagca cacggtaatt cgccggtgcg acgggctgca ggatttcgga tcggccggcg
     1201 gcgagctcgg catcaacgca ttgcggccgc acggagccga gtcgctggcg gatcagtgcc
     1261 atcagtcgtt gaattcaacg atgtcgccga tctcgggtcg gatttcctgg gtcggcgcct
     1321 tgatcggcgt cagcacgacg gttttggcct ggaaggcgcg ccggccggcc gaggtctgcc
     1381 gtcgccgctc gcgttcatac caaccctcgc gcatgacatg cagcaccgga tccttgagcc
     1441 gctgcgaggc gcgcttggcc ttgtattcca ggttgatctc ctgcagccgg gcatccagcg
     1501 cgcgcagaac ctggcgggcc tgttcgcgcg ccggcgcggc gatcggttca atgcgaaaca
     1561 gatagcgctc gcgatcctgg tcggcctcta cgcgaaagtg cgcgatcgtc agccccgatt
     1621 cgcgcgacac cgtctcgaac gtctggatca cctgattgac gctgattttt tcgccggtca
     1681 ggttggtcat gccgcgtccc ttgcgcagga agacgacctg gggcgttttg ttgtaatatc
     1741 cctgcacctg cacgacatca ttgatatcgt aacggtatag cccgccgctc gtggtgacga
     1801 agacgtagta ttccccttga tcctgaatct gatccaccgt cagaaaggtc caggcggcag
     1861 gatcgtccgg attggcatcg aattgatcca cgctgacgaa ttcaaacagg ttggtggcga
     1921 cggtcagcgg gccggccgag ccctcgtcgc tgagcgggat cgagccgcgc atttcgctgg
     1981 ccaggtatcc ccagtcgcgg accgggatcg gacggcgccc gtcgggattg aaccagtcgt
     2041 cgaatttggc cagatagtgg ccgaccgtcc cccccttcca gcaggcgatc agactgagtc
     2101 ggggccagta atccgccggg gcgaggcggc cgtcgcgggc cgaccgcgcc cgttcgagct
     2161 cgcgcgcccg ctccggattg ggccgcagca tcgcctcgac ctccttgcgg atctcgggtt
     2221 cgagctcaag ggtcggggac agcgtgccgt cgcggatatc gcgaagaata tcctcggaaa
     2281 actcattgcc tttttcgcac attttcagga tggacgatgg attggccgta ttcagaaagc
     2341 ggacatcgtc ggcaaccgcc agccgcatga tgacgtaata cttcgcgctg taatcggaaa
     2401 tttcgaacac cggatacggg attgaatacg ttttgcgaat gatcgccggc atattctggt
     2461 aaatgtgtcc cgacgtggag ccgaagggga cgcccgaggg ggcatggcct tccaccgccg
     2521 gcgaaaccag ggagaccact ttcgcatcga gaatatcggg atgagcgcac tgactgtgat
     2581 agagccaggt gcgcatgacg tcgcggtggt cgcgtccgta atcggtgggg gtgacgggta
     2641 tgaacttggg cgtgccggtg gtgccgctcg tctgcgcgaa catgatcggc ttttcggcgg
     2701 tgaacacgtt ctccgcgcct tcgaaaacac gttgcatatc ctcctggata tcctcgtacg
     2761 aggccaccgg gatctggcgc tggaagtcgg tgaaggtgcg gacggatgcg aagccgtggc
     2821 gccggccata ttccgtatcc ttgttcttgt ccatcagcca gagcaatttg cgttgttgcg
     2881 cctcggccgg gtcccgcgtg gcggtttcaa aacgccgggc cgaccgccgc atcgttaaga
     2941 taatgagttt cgtccggacc gccatcgctc ttacccactt ccccgggatg aacccggccg
     3001 tgccgcatcg agcccggtga tccactcatc catcgcgcgc gcgattgcgg cggggatttc
     3061 gaactggagt gaatgcggca ggcccggata gcgccggatc gtgatcagcg gcgattgccc
     3121 gcgggccaat aaggccgcgg tggccgcgtt gtcgataatg acgtcgtcgc cggcgagcag
     3181 cagcagcacg ggcgtcctga attcgccgat ttcagactcg atctggcgat ccagacgccg
     3241 gctttcccac aggaagcggg ccgtggcctc gcgcagtcgc agcggatcgc gctcgatccg
     3301 agcgagaaaa tgcggattgc gcgtgaacat ccagggctcg atcggcgttt ggaacagcgc
     3361 ggttggccgg aacggctggg caaacagaat gccgattttt tgcgccggcg tcacatcgac
     3421 gcgcggcacg agccccggcg tgacgagcac cagaaagtcg aaatccgccg ggtgccggag
     3481 cgcatggccg acggcgagtt tgccgcccca cgacaacgcc accagcccca acggcccgtc
     3541 cgggggcagc gccgcgcgga atgtgtggat gtcgccgatg agtgtatcgg catgatcaac
     3601 atgtccggcg cgcagaccgg ggccggactg attgagcccg ctcccccggc gatcgagaag
     3661 atagagttca tagcccgagc gcgcgagctc ccgcgccggc gcctcaaacc agccggcgtg
     3721 gctttcgatg ccattgaggt aaacgagccg gacgccattg gcccgctcgg gggggtagtg
     3781 cacgaagcgg aggcggacgc cgtcgcccgc cgcaaaatct tcaacgagcg gctgaatcgc
     3841 ggaatccccc tcgtcataga aatcaacggg cgtccgcgac gccgcccgtt gcggaacgcc
     3901 ggcgcagccg gccagcaggg ccgccggcag catggtgaga acgagacgga tggggcgcgg
     3961 gctcatgagc tgaagatcgt ctccgccatg tcgtgaattg tccgcgatac ggcgtgctcg
     4021 ggctgcgccc gcgcgatgat ctcgcgactc tgcaaccggg ccagatcctc gctgagcggg
     4081 agcacgccga tcacgggatg gtcgaaggct tcggttactt tcgtggtcag cgccgccgta
     4141 tcggcctgcg agtagacctt gttcaggatc agatgaatcc gccggatctc cagtttcgcg
     4201 gccagttcat tgatcaccgc cgtcccgtaa tagtcctgct gatcgggccg gaggatgagg
     4261 atcagttgat cggagatggc cgtggtcagg agcgtttcgc ggttgaggcc cggatgcgta
     4321 tcgatcagca gccagtccag gtcgaggtac tccagcagtt gatcgaagtg tgaattgagg
     4381 cgctggacgt cgtagccctc ctccaggacg cgcgtgatcg cctgcgccgt cagcgatgcc
     4441 ggaatgagcc agcatttgcc atcgcccggc gggcggagcc gttccgtcac gtcataggcg
     4501 gtttcctcca gcccgcactt gccccagagg aagtcgacga gcgtgagctg gatctgatcg
     4561 cgctcaagtc cgaagagaat atgaatgccg ggcgaaacga gatcgcaatc gagcacggcg
     4621 acccggtcgc cgcgcgccgc cgcgaaccag gcaaaattgg cggcgagcgt ggacttgccc
     4681 gttccgccgc gatacgaatg gagcgagatg atgcgccgcc gaccggtcat ggccgctcca
     4741 ccccgacggc cggacggccc tgctgcatga cggggagcgt gcgggccccc gtatcgacct
     4801 cgcgagggtc ggcctgggtt tccggatcgc ctggcgccgg atcgatctgg tcggcgtggc
     4861 gttgccacca ctccacggcg gcggcccggc tccagggatg ctccagccgg agctgctcca
     4921 gctcgtcgat gatctggtcg acacgctgcg gccgatcatc cgggttcttg gccaggcagc
     4981 gcaccaccag ctcatccaac tgcgccggaa tcggctgctg cgccacgctg gacggtcgcg
     5041 gcaccggcga ggccagcgtc gcctggctga gttccatcgc cgtggcgccc tcgaacagct
     5101 cctgggccgt cagcaggttg aatgccacga cgccgagcga gtagatatcg gttcgcgtat
     5161 cgacccgcgt gggatcgcgc agccgctccg gcgcgatata gagcggcgtg ccgttgatca
     5221 cgttggtcga cgtcaccgcc ggttcgccgt cgatcgccag ttccttgacc agcccgaaat
     5281 ccagcacctt gacgaaatcg ggttcgccgc ccagatcgca cagcatgata ttgagcggct
     5341 tgatatcgcg gtggatcagt tgatgggcgt gggcctcgcg cagcgacccg cagacctggc
     5401 gcaggatgtg aatcacgcgg tcgggcggca cctcgccctg cagctcgagc aggcgcgcca
     5461 gtgtgatgcc gtgcaggtat tccatcacat aatagaaaat cccctcatcg gtcaccccgt
     5521 ggtcatagat ctcgatcgtg ttggggtgcg ccaactggct cgtcaactgc acttcgcgct
     5581 cgaagcgctc aagcgtcggc gtatccgact ggtccgtgtg cagcagcttg atggcggttg
     5641 cccgccgcag cagggcatgg cgcgccaggt agacctcacc catgcccccg ccgccgatct
     5701 tccgctcgag ggtgtatttg tcgatgcgct ggcttgcccg gatgtcgcgc cgcagtcggc
     5761 tgatgatcgc attggtgacc agaatgccga gcatcaccag caccagcagc cagaagagga
     5821 tgcgaaacga ataggtcagc gggcggatgg cgtccagggc ctcctgaata ctgacttcgg
     5881 cggccaggcc cagatcgtaa tccggcagcc agcgccaggc gcccagcacg gggacgccgc
     5941 ggaaatcgcg atagccctcc aggttgtagc cgttctggcc cgacgtggcc gaggcggcca
     6001 tcgccgtcag cggccgggcg gcgggaagtc cgtcgggttg aaaaccccgc gtcaggtcgc
     6061 ctcccggatc gcggatctcc aggttcagga tcgactgcga ctgattcgta ttggggagca
     6121 ggccgatctc cttgagctgc tcctcgaagc ggctgtcgga gaggatcacg ccgttgcggt
     6181 tgaaggcata ggtgttgccg gtcgcgccga cgcgcgcaat cgaaatgatc cgcgtgaatt
     6241 cctgttccgg gttaatcagc agggcgatgg ccccgatcgg ctgctcgtca tcggtctcca
     6301 cggcggccag cgacagcatc accggtcgat tgacgtcgac aacctggccg ctgacatggc
     6361 tgcccgtgtg cagcgggtgc gagacgaacg gccccttctc gaataccttc cccagcaggg
     6421 ccatcccttc gccattaagc cgtcggccga ccggcgggtc gtcgtgcgcg gcgaggatca
     6481 gtcccgtccg gtcgtacacc tgaaagccga catggttttc gtcctcgtga atgagcgaga
     6541 ggaaccgccg cagttcctgt tgcgcggaat cggcgagcag cgtatcgctg gtcgtcgtcc
     6601 ggtcggtcgc cagcatgctc aacgcggcaa cccgctcgac caccgccgga tgcgccgccc
     6661 agcgccgcac ttcctgctct tcctcctcaa accacatctg cagcgcggcg acgtcggcct
     6721 ccagcacggc gttgagatat tccgcaagca cctgcttgag cgccgtctcg atcgcgttgt
     6781 gcgtggcgaa gccgagcagc gccaggaacg agcccatgca gaaaatgaaa aagaccagtt
     6841 tcttgttctg gcgaagcaag gcgaagcggc ggcgcgggag gaggcgttcg aggaacccgg
     6901 cctggtccgg gcggttgcct ggcgtgtcga tcacggagag ctccttttcg ccgttgccgg
     6961 cgcatcctat gtccgcgaaa tacagggatc gccaatcctg atccgtcccg gaatcgatgg
     7021 gtcaaccgat ccggcagctt cggacggaat acctcctcag cgccgcttca ttcagagcaa
     7081 taccgatgcc agattcagga tcgtaccgag gggcgcggcc ccccggccca aaggcgaacc
     7141 cgtggcccga tgcccagccg gagagatcat ccaccagcag gcgcgtgccg aaactcccct
     7201 ccatgaacac gaccggcgcc agccgccggg cgaccgccag cccgacggcc gagagcagcg
     7261 ccgattcgcc cacctggcat ccgacctgaa cctccagccc atggcgccgg gcgaaatgcc
     7321 ccagccgcag ggagttgaaa aaaccgccgc acttcgacac gcggatgttg aacacctggc
     7381 acgcgccggc ctccaccagg gtctcggcat cgcgcaggtc gcacagatcc tcgtccgcaa
     7441 tcaaccgcgc gccggtttca tcggccaagc ggcggcacaa ttcgtgacgg ccgcgcgcca
     7501 gcggctgctc gacgtagtgc acggcgtggc ggcgcgccat catgatcgcc cggcgtgcct
     7561 catcgaactc ccatcccata ttggcatcca ttcttaaaga cacggccggc cccaggattc
     7621 ttcgcgcaac gcgcagcacg gcgccgtcgc ggggcgtacc ggccttcagc ttgacggccg
     7681 gcacgcccgc ccggcggcag cgccaggccg taactgccgc cgcgacgatt cccgagctgg
     7741 aaatcacgcc cgaatagacc ggatccgacc ctgcgccgga atgggcgaac gacgtcgcgc
     7801 cgaaatgacg accgtaggcg tcgagcagcg ccagttccgc cgcgcagcat gccgcgccgg
     7861 gtcgctccgc gattcccgcc gcgccgctca ccagccgctc atacagtccc ccgcagaaag
     7921 cttcgacctc gccaatggtc gcaaagtcgc aatcaagcaa cggtggcagg atgcgctgcg
     7981 tcagattctg gaaacacccc tcccagctct cgccggtgac gtactcgcgc ggcaagcctt
     8041 cgccatggcc gacggcgccg tcggccagtt cacagcgcac gaagatgctc gcgctgtggc
     8101 tgcgactggc ctgcgcgtgc cgaaaccgga cgcggaacgg cagcgcggtc tgaaacactt
     8161 cgaaatggcg gatcctcatg cttgctttcg cccggatgcg gcctccccgg cacgctgatt
     8221 gctcttttca tgccagaagg atcggccgac atcatggaaa cgacgattat ctcgaccgac
     8281 aagattgacg ttgcgctcgg gcatcaggag ctgatggccg tgcggacgtc cgacggcgtc
     8341 gtcattccga tgcgctactg gcccggacgc gagcagcgcg gcgccgttct gttcgtccac
     8401 ggcatgctca gccattcggc ctggttcatc cccaccggcg actggctgaa tcaactcggc
     8461 ttcacctgcg tcgcgctgga tcgccgcggg tcgggacgca gcgaggaggc ccgcggccac
     8521 atgctctcgt accaccagct ggtggatgaa atcgaggggg cggtggggtg gctggagagc
     8581 gagcaccccg gcgcgccgat tcacctcgcg gggcagtgct atggcgctct gccggttctg
     8641 cgcttcgccg cggcgcatcc cgaacgctgc cggtcgctca cgctgctctc gccctcgatt
     8701 cgcgtccacg cgacgctcac gctgcgcgag aagctgctcg tcgccctgct ggcgaaggtg
     8761 cggccgacgc acccgatgcg cgttccgctc aagccggagc atttcaccga cctgccggaa
     8821 cacctcgaat tcatccgcgg cgatgccctg cggctacaat atgtcaccac ggcgttctat
     8881 ctgcagacgc tgctgatgat gctcgccgtg cggggctcgg ccgaccggat cgtgacgccg
     8941 acgttcatcg cgctggccgg gcgcgacgag gtgtgcgaca acgccgccgc cgagcgcttc
     9001 ttcagccagc ttgcggcgga agacaagcgt ctgacgacct accccggctc aatgcacagc
     9061 ctggagtatg gccgcgaccg tctcgcgttc ctcgctgatt atcgtcactg gctggcggat
     9121 cacgaccaga tcgtgagttg aatcgccggc ggccggttca acccaggatg cagcaaggat
     9181 gatggaatca gaagcccgtc gaagggctgg atgcggttgc agggaacgcg tgcgtagcta
     9241 tgtcgcccgg cggtcgcgca gtcgcacgcc acgaactcaa atctccgagg cgcgattggc
     9301 gcgaggaccg cgccacggac cgccgggcga cgcgccagct actcaaccgc cagggcgcag
     9361 agttggcaga gacggccgtc gtgccagacg ccgctgccgg tgatccgcgg gtcgtcgagg
     9421 gccagcccga ttccggcgcc cgtctggttc cagcggaccg tcaacgaatc ggccacgcgg
     9481 cccaggaacc agcgcagcga cagctccgac agttccgccg gtcgctcttc ctcgggcggg
     9541 cggcgtctgc gcagtgcttc cacgtaatag ccatcggcca gtttgtccca ggtcgtccgg
     9601 aagctggagg gatgatccat cagttccagg ccgaggatgc ggtcatcccg cgcgatgacg
     9661 acaccgaccg cttcgcgcgg cagcatgagc gcctcgcgag tggtccgcag ccgctcgcgt
     9721 tgcgtgtcat acaccgcgtc caggtcatgg gttggcgacg gcgcgtccat ctcgcgcagg
     9781 caggcctgcc cctcaaccca gacctcgccc tgcgccgcgt tcgcgtagcc ggcgtcgcgg
     9841 cggcgcaata gcgtc
//
LOCUS       DAOPCO010000147         9812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6621_length_9812_cov_5.037102, whole genome shotgun sequence.
ACCESSION   DAOPCO010000147 DAOPCO010000000
VERSION     DAOPCO010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_9812_cov_5.037102"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PLS90_10775"
     CDS             complement(<1..1371)
                     /locus_tag="PLS90_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02927.1"
                     /translation="MLISLAAAIRAVPLVANGVWFDELYSIKIATFGLRDIAWWVRYD
                     THPPLYYQLLHVAMRAAELLGGGPLISTDWLRLTSILPSLAVCFILGGWAWRASGRVA
                     GLTVFALAAFNPGLCFYALELRNYAQTMMFVALATLSLVMLLRRPGPLWMLTFAAASL
                     GHLYTHSLTTLVLAAHGLWVLAQAIPPRRPGGIRLRVYVYGAFALVAAIYLPQLAHVW
                     DQFVHQYKPVEELLEDPSWGDLLLLLFYAYPASPMMTQSWWTPYGIVMSYLYIVLGVL
                     LARELIRARRPWPRGSLARESVAASLFVLAFFILVTWWLTLAGVGEFFSAQRLAMVVL
                     PLWLLAVGLLIERIGRGRARRLGRVFVVGLFIPAAIMALIEKAAYRTDFLPLARYNRE
                     LHEAEGARLPRTALLADERLAPWIRRPHPLVRLRGLDENLAQPRIPLVTEVATLASIS
                     GRIQTDR"
     gene            complement(1707..3173)
                     /locus_tag="PLS90_10780"
     CDS             complement(1707..3173)
                     /locus_tag="PLS90_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPK02928.1"
                     /translation="MHPRKTIRLALGALTLAAALSLGRIAAEGPPPAGGTLPLYFSGD
                     REGGIDPCGCPSIEQGGVVHEAGLYPAGESLRLDAGGWTSALMFSEPDAWLRSQFLLR
                     GLAEMQYDAVNVGQIDIHLTRGWYDHLRQEYPLAYEPLISANTFLKDAHETRAFAAYR
                     LLERRSGAGERILVGVTGAVSTERSADGTGEPPEELETESFVVRDPAAVLPGVVRELR
                     AAGAAVVIVLFDGDDRQTQRMRQAAADADVLITTGDAPAGGELRAAGPTVLRTPHTRG
                     RQVGHAEVEIAGGSLRRVVERPPLSVRVDLSADPTLELLVGEYQALAAQTAPAASGDA
                     GYAGVDACARCHEQPYRAWRETAHARAFRPLLEEGRMYDAEALRRRVTGWGEAGGFVS
                     VLDGPSRELLNVQCEACHGPGRAHAEMEARLAGAGRWLPAGEREALEREARQRRPPVQ
                     VPEATCLPCHDPARDPAFNFKRDIRRVDHDAGWLGKLF"
     gene            3231..4571
                     /locus_tag="PLS90_10785"
     CDS             3231..4571
                     /locus_tag="PLS90_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02929.1"
                     /translation="MIPQSSTARPTRREWLAAVALLALLGACALRPSIRGNDGHGHYV
                     YLASLLGDGDLDFADDYRDFDARRQYSYRFADLPVSDRTGRPSNRYGIGAALLWAPLV
                     VPVWLVLARTGDVNPLHPALAGAVGLATLLWGSLGLLLLYARLRRRWPVAPAAGAILA
                     LLLATPLGFYLYAHGSMSHGVGFFAVAAALLLFERAWRSPCPRSYGACGALLALIPMI
                     RFQDAVWVLILGGGLALKMAWEVRAGRVSAGKALLHAAALAAGFLLAFLPQMFVWRTL
                     YGSLLSGPMPYLTGSAGAFSPWPRHLLQVLVSERAGVLAWHPLILFALAGLAATLRDP
                     RLRLISAAGLTGFLLTLLLVASWSIWWAGASFGNRFFIGTLPALAFGAAWWLARPAPA
                     ARRAAWLVLGLLIVWNMGLLVQYATEMVPREDPVPWSQVIRQNLIDVPQRILNR"
     gene            complement(4664..5671)
                     /gene="pdxA"
                     /locus_tag="PLS90_10790"
     CDS             complement(4664..5671)
                     /gene="pdxA"
                     /locus_tag="PLS90_10790"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013109599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPK02930.1"
                     /translation="MEQAEIEKPWVAITQGDPAGIGPELCLRVMHDPSVRDVCRPVLI
                     GDDQVMRQAAAMLKLPPPQMTLTVDALLEGDGLGAMEAPVLVDCQMITAEVTPGRATR
                     RNGEASLRYIKVAIAAARAGRVRAVTTAPITKATLHMAGIDEPGHTEIFAHRTGTTNY
                     AMMLYSPRLAVSFVTCHQSLESVPGALTIDAICRVTDLTGRTLRRLRGAEPRLAVLGL
                     NPHAGEEGLFGRQELEIIAPAVVRSRAEGWQVEGPVPPDAAFMPQALERYDGHVTMYH
                     DQGSIPFKMVSLHDGVNITMGLPIVRTSPDHGTAYDIAWQGKADPASMRAALRLAAGL
                     A"
     gene            complement(5842..7635)
                     /locus_tag="PLS90_10795"
     CDS             complement(5842..7635)
                     /locus_tag="PLS90_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK02931.1"
                     /translation="MSQHRTNFFRFLRYVLPYKKYIVLAALGGMVKFTVPLLFPKLTQ
                     HLIDEVFGNAALTPAEKTRELLTLSGGLIALYLVVYAPWTYQRHYFAGKAGHRSVFDL
                     RCDLYYRILRMSHSFFSRNKSGAIVSRLISDIQLAQNLVGSALTNVWMDACALIVLLW
                     FLISIDPPTTAVALATMPLYLYFFKKFGRKIKSASYDVQQEIATMSGNANEKIAGSTI
                     VHAFTQEAREEKAFLRESEKLFLKVMRTITLQGMNITCMSTLTNIAPVIVMLYAGMRV
                     IGERMTGGELVAVLMYLGPLYLPLQRFSELNVIFANSMAAIDRIFEIIDEKPEIADRP
                     NAAALPDVRGRVEFQDVVFGYHDDQPVLRGVSFVAEAGAKVALVGRSGSGKSTLISLI
                     PRFYDVHSGAVRIDGHDVREVKVKSLRQHIAVVLQDPILFSGTIVDNIRYGRPEASDE
                     EVLRACRDANALDFIHELPDGLNTEVGERGGFLSGGQRQRITLARAFLKNPRILLLDE
                     PTSALDSESEHLIQDALERLMKGRTTFIIAHRLSTITGADTVLVMEDGRIVEAGSHRE
                     LLLAGGIYRQLCEQQYGFARQSIEFISGIAG"
     gene            complement(8063..8764)
                     /locus_tag="PLS90_10800"
     CDS             complement(8063..8764)
                     /locus_tag="PLS90_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK02932.1"
                     /translation="MRRSPLMGCVLALTLLLAAHAATAATLVNSWTATGVAGALSPPN
                     YGLRLDGFFDNNVNHEVTFAFDNVTFNEFDDGSARLFGTISIAEYDNSGGPGAFASTW
                     DLDVAFQQTTGSNASWRYYNLISNGRELENTGNSNDYANFFQFMGPFQVGNGANGKNA
                     NFGAAGWVNYVHFLAGGGTTGSESTHYYSSDFLMDLTRKDIPVIPEPATLSLIGLGLG
                     SLAAGRRFRRSAARS"
     gene            complement(9064..9252)
                     /locus_tag="PLS90_10805"
     CDS             complement(9064..9252)
                     /locus_tag="PLS90_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02933.1"
                     /translation="MLPRSRLVRWLAVNAAFWAFLVWSVWLWRLGLPRGTSLHLIALA
                     WAIPFGVISLLLVRRGLP"
     gene            complement(9349..>9812)
                     /locus_tag="PLS90_10810"
     CDS             complement(9349..>9812)
                     /locus_tag="PLS90_10810"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="catalyzes the phosphorylation of
                     N-acetyl-L-glutamate to form N-acetyl-L-glutamate
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPK02934.1"
                     /translation="IKKAHRAAPGDVGEVEQVNVEILRILERTNYIPVISPIGAAADG
                     QTYNINADTVAAQCAMALQARKLILLTDVRGICRDPRDPATLLNRVAVEEIEPLIREG
                     VIKGGMIPKVRACRDAIAAGVASAHILDGRLPHALLIELFTDRGIGTMITA"
BASE COUNT         1584 a         3628 c         2990 g         1610 t
ORIGIN      
        1 gcggtcggtc tggatccgcc cgctgatcga ggcgagcgtc gcgacctcgg tcaccagcgg
       61 gatgcgcggt tgcgccaggt tttcatccag tccgcgcagc cgcaccagcg gatgcggccg
      121 gcggatccac ggggccagcc gctcgtcggc cagcagcgcc gtccgcggca gccgcgcgcc
      181 ctccgcttca tgcagctccc gattgtagcg cgccagcggc aggaaatcgg tgcgataggc
      241 ggccttctcg atcagcgcca tgatggccgc gggaatgaac aggccgacca cgaagacccg
      301 cccgagccga cgcgcccgcc cccgcccgat acgctcgatc agcagcccca ccgccagcag
      361 ccacagcggc agcacgacca tcgccagccg ctgcgccgag aaaaactccc cgaccccggc
      421 gagcgtcagc caccacgtga ccaggatgaa gaacgccagc acgaacagac tggccgcgac
      481 cgattcgcgc gccagcgacc cccgtggcca cggccgccgc gcccggatca gctcgcgcgc
      541 cagcagcacc cccagcacga tgtacagata cgacatcacg atgccgtagg gggtccacca
      601 cgactgcgtc atcatcgggc tggcggggta ggcgtaaaac agcagcagca gcagatcgcc
      661 ccacgaggga tcttccagca gttcctccac cggcttgtac tgatgcacga actggtccca
      721 cacgtgtgcc agttgcggca ggtagatcgc ggcgacgagc gcaaacgcgc cgtaaacgta
      781 gacgcgcaac cggatcccgc ccggccggcg cggcgggatc gcctgcgcca gcacccacaa
      841 cccgtgcgcc gccagaacga gcgtggtcag gctgtgcgtg tagagatgac ccagcgaggc
      901 ggccgcgaac gtaagcatcc agagcggtcc cggccggcgc agcagcatca ccaggctcag
      961 cgtggcgagc gcgacgaaca tcatcgtctg cgcgtagttg cgcagctcga gggcgtaaaa
     1021 gcagaggccg ggattgaacg cggcgagcgc aaaaaccgtc agcccggcga cccgccccga
     1081 ggcgcgccac gcccaacccc ccaggatgaa gcagaccgcc aggctgggga ggatcgacgt
     1141 cagccgcagc cagtcggtcg agatcagcgg cccgcccccc aggagctcgg cggcgcgcat
     1201 cgccacgtgc agcagttggt aatacagcgg cggatgcgtg tcgtagcgca cccaccacgc
     1261 gatgtcgcgc agcccgaacg tcgcgatctt gatcgagtag agttcgtcga accagacccc
     1321 gttggcgacg agcgggacgg cgcggatcgc cgccgccagc gagatcagca cgggccagat
     1381 ccacagcagc gagcgtctga cagaacgggc ttgcctgtgg cgctggattt gatccgccgg
     1441 ggatggcatc aagggtcgtc tcactctaga atcgtctctc gtccctcgtc ctcgtgatcg
     1501 ctcccaaagg cccacccagt ttttgtcctt tcgtcgtgaa ctgccggttc acggtaaggg
     1561 caaccaccgc ctgggaccgc cggcatcttg gccggcatct acccccaacg gccccccagg
     1621 aattcggctc tcggctctcg tctcggctct cgacaaccag ccattctgtc ctttcgttgt
     1681 gaaccgccgg ttcatcggca actcactcag aagagcttcc ccagccagcc cgcgtcgtga
     1741 tcgacccggc ggatgtcgcg cttgaaattg aacgcggggt cgcgcgcggg atcatggcag
     1801 ggcaggcagg tcgcttccgg aacctggacg ggcggacgcc gttgccgcgc ctcgcgttcg
     1861 agcgcttcgc gctcgccggc gggcaaccag cgccccgccc cggccaatcg ggcttccatc
     1921 tcggcgtggg cccgcccggg gccgtggcac gcctcgcatt gcacgttgag cagttcgcgg
     1981 ctcggcccgt caagcaccga gacgaatccg ccggcctcgc cccaaccggt tacgcggcgc
     2041 cgcagcgcct ccgcatcata catccggcct tcttccagca gcggccggaa tgcccgcgcg
     2101 tgcgccgtct cgcgccacgc acgatacggc tgctcgtgac agcgcgcgca cgcgtccacg
     2161 cccgcgtagc cggcgtcgcc gctcgcggcc ggggccgtct gcgcggccag cgcctggtat
     2221 tccccaacca gcagctccag agtcggatcg gcggaaagat ccacccggac cgacagcggc
     2281 ggccgttcca ccacgcgccg caggctcccg cccgcgatct caacttccgc atgccccacc
     2341 tggcgccccc gcgtgtgcgg cgtgcgcagc acggtcgggc ccgccgcgcg gagttccccc
     2401 cccgccggcg cgtcgcccgt cgtgatcagc acgtcggcgt cggccgccgc ctgccgcatc
     2461 cgctgcgtct ggcggtcgtc gccgtcgaac agcacgatca ccaccgcggc gccggccgcc
     2521 cgcaactcgc gaaccacccc cggcagcacg gccgcgggat cccgaaccac gaaactctcg
     2581 gtctccagtt cctcgggcgg ctcgcccgtg ccgtccgccg agcgttccgt cgaaaccgcc
     2641 cccgtcacgc cgacgagaat ccgctcgccc gcgccgctcc gccgctccag cagccggtac
     2701 gccgcgaatg cgcgggtctc gtgtgcatcc ttcaaaaacg tgttcgccga aatcagcggc
     2761 tcataagcca gcggatactc ctggcgcagg tggtcgtacc agccgcgcgt cagatggatg
     2821 tcgatctgcc ccacgttgac cgcgtcatac tgcatttcgg ccagaccgcg cagcaggaat
     2881 tgcgacctca gccaggcatc gggttcggag aacatcagcg cgctggtcca gcccccggcg
     2941 tccaatcgca gcgactcgcc ggccgggtag aggccggctt catgaaccac gcccccctgc
     3001 tcgatcgacg ggcacccgca gggatcgatg ccgccctcgc gatcgccgct gaaataaagc
     3061 ggcagcgtcc cgcccgccgg cggcggaccc tccgcggcga tccgccccaa cgacagcgcc
     3121 gccgccagcg tcagcgcgcc gagcgcgagg cgaattgtct tccgcgggtg catcggttag
     3181 cttgaactcc cgatcgacgt ggctcgcccg aagggacgcg attgtagacg atgatccccc
     3241 aatcttccac cgcgcgcccg acccgccgcg aatggctggc cgccgtcgcg ctgctggcgc
     3301 tgctgggcgc ctgcgcgctg cgcccctcga tccgcggcaa cgacggccac ggccactatg
     3361 tgtatctcgc ctcgctgctg ggcgacggcg atctggactt tgccgatgat taccgggatt
     3421 tcgacgcccg gcggcaatac tcctaccggt tcgccgacct ccccgtctcc gaccgcaccg
     3481 gccgccccag caaccgctac ggcatcggcg cggcgctcct ctgggcgccc ctggtcgtcc
     3541 cggtctggct ggtcctggcg cggacgggcg acgtcaaccc gctccatccg gcgctggccg
     3601 gagcggtcgg gctggcgacg ctcctctggg gcagcctcgg gctgctgctg ctctacgcgc
     3661 gtctgcgccg ccggtggccg gtcgcgccgg cggcgggggc gatcctcgcg ctcctgcttg
     3721 ccacgccgct cggattttat ctttacgcgc acggctcgat gtcgcacggc gtcggctttt
     3781 tcgcggtcgc ggcggccctc ctgctcttcg agcgcgcctg gcgctcccca tgcccccggt
     3841 cgtatggcgc ctgcggcgcc ctgctggcgc tgatcccgat gatccgattc caggatgcgg
     3901 tgtgggtcct catcctcggc ggtgggctgg cgctgaaaat ggcgtgggag gtccgcgccg
     3961 gccgggtttc ggccggcaag gcgctgctgc acgccgccgc cctcgccgcc ggctttctcc
     4021 tcgcgttcct cccgcaaatg ttcgtctggc ggacgctcta cggctccctg ctgagcggcc
     4081 cgatgcccta cctgaccggg tcggccggcg ccttcagccc ctggccgcgc catctgcttc
     4141 aggtgctcgt gagcgaacgg gccggcgtgc tcgcgtggca tccgctgatt ctcttcgcgc
     4201 tggcgggact ggccgcgacc ctccgcgatc caaggctgcg cctgatctcg gccgcgggat
     4261 tgaccggctt cctgctgacg ctgctcctcg tcgcgtcctg gtcgatctgg tgggccggcg
     4321 cctcgttcgg aaaccggttc ttcatcggca cgttgccggc cctggcgttc ggcgccgcct
     4381 ggtggctggc ccggcccgct ccggcggcgc ggcgcgcggc gtggctcgtc ctcggcctcc
     4441 tgatcgtctg gaacatgggg ctgctggtcc agtacgcgac agaaatggtc ccgcgcgagg
     4501 acccggtccc ctggagccag gtcatccggc aaaacctgat cgatgtccca caacggattc
     4561 tgaaccggta acgggcgacg gaaccggcga cgttcgacag ccgaacttta agaaagcatc
     4621 cccggcccga tctgtccctt acgtcccttt ccccccccgc tccctacgcc agtccggccg
     4681 ccagccgcag cgccgcgcgc atgctggcgg gatcggcctt gccctgccag gcgatatcgt
     4741 aggctgtccc gtggtcgggc gaggtccgca cgatcggcag ccccatcgtg atgttgaccc
     4801 cgtcgtgcag cgaaaccatc ttgaacggga tcgaaccctg atcgtgatac atcgtcacgt
     4861 ggccgtcgta gcgctccagc gcctgcggca tgaacgcggc gtcgggcggg accgggccct
     4921 ccacctgcca gccctcggcg cggctgcgca ccacggccgg ggcaatgatc tccagctcct
     4981 gccggccgaa cagcccctcc tcgcccgcat gtggattcag ccccagcacg gccagccgcg
     5041 gttccgcccc gcgcaaccgc cgcagcgtcc gccccgtcag gtcggtcacc cggcagatcg
     5101 cgtcgatcgt cagcgccccc ggcacgctct ccagcgactg atgacacgtg acgaaactga
     5161 ccgccagccg cggactgtac agcatcatcg cgtagttcgt cgtgccggtc cggtgcgcga
     5221 aaatctcggt gtgccccggc tcgtcgatac ccgccatgtg cagcgtcgcc ttcgtgatcg
     5281 gcgcggtcgt gacggcccgc acgcgcccgg cccgcgccgc cgcgatcgcc actttgatat
     5341 accgcaggct ggcttcccca ttgcgccgcg tcgcgcgccc cggcgtcacc tccgccgtga
     5401 tcatctggca atcgaccagc accggcgcct ccatcgcacc cagcccgtcc ccctccagca
     5461 gcgcatccac cgtcagcgtc atctgcggcg gcggcagctt gagcatcgcc gccgcctgtc
     5521 gcatcacctg atcgtccccg atcagaacgg gccggcacac atcccgcacc gacgggtcat
     5581 gcatcacccg cagacacaac tcggggccga tccccgccgg gtccccctgc gtgatcgcca
     5641 cccacggctt ctcaatttcg gcctgttcca cgcagtccac cttgctcccc tccgctcaca
     5701 ttataatcgt ccctcgtcct cggctctcga acccttcggc gccgggccaa cattctgctc
     5761 atccattcct gtcgtgctca tccgtgactt ccgtgatcct gtttcctcgg aggagacgaa
     5821 cctccccccc gccgtcaacc cctaccccgc aatgccgctg atgaattcaa tcgactggcg
     5881 cgcgaacccg tactgctgtt cacaaagctg gcgatagatc ccgccggcca gcagcagctc
     5941 gcggtgtgag cccgcttcca cgatccggcc gtcctccatc accaggactg tgtcggcgcc
     6001 ggtgatcgtc gacagccggt gcgcaataat gaacgtcgtc cgccctttca tcagccgctc
     6061 cagcgcgtcc tggattaaat gttcggactc cgaatcaagc gcggaggtcg gctcatcgag
     6121 gagcaaaatc cggggattct tcagaaatgc ccgcgcaagc gtgatccgct ggcgctgccc
     6181 gcccgagaga aatcccccgc gctcgccgac ctcggtgttc agcccgtcgg gcaattcatg
     6241 aatgaaatcg agggcgttgg cgtcgcggca cgcccgcagg acctcctcat cgctcgcttc
     6301 cggccgcccg tagcggatgt tgtccacgat cgtcccgctg aacaggatcg gatcctgcag
     6361 cacgacggcg atgtgctgac gcagcgattt gaccttcacc tcgcgcacgt cgtgcccgtc
     6421 gatccgcacc gccccgctgt gcacatcata gaaccgcgga atcaggctga tcagcgtgct
     6481 cttgcccgac ccgctccgcc cgaccagcgc gaccttcgcc ccggcctcgg ccacaaagct
     6541 caccccgcgc aggaccggct gatcgtcgtg gtagccgaag acgacgtcct gaaactcgac
     6601 gcggccgcgc acgtcgggca gcgccgccgc attcggccgg tcggcaatct ccggcttctc
     6661 gtcgatgatc tcgaagatcc ggtcgatcgc cgccatcgag ttggcgaaga tcacgttcag
     6721 ctccgaaaac cgctgcagcg gcagatacag cggccccaga tacatcagca ccgccaccag
     6781 ctcgccgccc gtcatccgct cgccgatcac ccgcatcccg gcgtagagca tcacgatcac
     6841 cggcgcgatg ttcgtcagcg tcgacatgca ggtgatgttc atcccctgca gcgtgatcgt
     6901 ccgcatcacc ttgaggaaca gcttttccga ctcgcgcaga aaggccttct cctcgcgcgc
     6961 ctcctgcgtg aacgcgtgaa cgatcgtcga gcccgcgatc ttctcgttcg cgttgcccga
     7021 catcgtcgcg atttcctgct gcacgtcgta gctcgccgat ttgatcttcc gcccgaattt
     7081 cttgaagaaa tacagataca gcggcatcgt cgccagcgcc acggccgtcg tcggcggatc
     7141 gatgctgatc agaaaccaca gcagcacgat cagcgcgcag gcgtccatcc agacgttggt
     7201 cagcgcgctg ccgaccaggt tctgcgcgag ctgaatgtcg ctgatcagcc gcgagacgat
     7261 cgcccccgat ttgttccggc tgaagaacga atgcgacatc cgcaggatgc ggtaatacag
     7321 gtcgcagcgc aggtcgaaca ccgaccgatg gcccgccttc cccgcgaagt agtggcgctg
     7381 atacgtccac ggggcgtaga cgaccagata gagcgcgatc agcccgcccg acaacgtcag
     7441 caattcgcgc gttttctccg ccggcgtcaa cgccgcgttg ccgaagactt cgtcgatcag
     7501 gtgctgcgtc agcttgggaa acagcagcgg aaccgtgaac ttgaccatgc cccccagcgc
     7561 ggccagcacg atgtatttct tgtagggcag cacatagcgc aggaagcgga aaaaattggt
     7621 ccggtgttga ctcatgatct ggcggcgagg cccgcgcagc gcgcgcgacg gcgcgggcgc
     7681 gggcgagtgg cacgcacgga atcgcgctag attcgtccgg cggcgctgcc gcggaccttc
     7741 gcgatcccgc ccgtagaggc cggtattttc ccacatcccc gccccaatgg caacccgccc
     7801 cggcattcat ccgtatcgct atcggtattc gactccgctc cccgcctgat tcctcaagga
     7861 acccggctct cgcccgagcg gttccgtatc ccaaaaaaat ctcggccgct cgctggctaa
     7921 cgcggcggcc gggcggtttg ccgtatcgta ttaaagcggt cataacgcgg cgaggctccg
     7981 cgcgaccggg gcgtcggcgg agcctcgtca tcttcatctc aacatcgatt caacctcaac
     8041 gcgcgttgcg gacctcccgt tctcagctcc gcgcggcgct gcggcgaaac cggcggccgg
     8101 ccgccagcga gcccagcccc aggccgatca ggctcagcgt ggccggctcg gggatcaccg
     8161 gaatgtcctt gcgggtcaga tccatcagga agtccgacga atagtagtgc gtggactcgc
     8221 tgcccgtcgt ccccccgccg gcgaggaagt gaacatagtt gacccagccg gccgcgccga
     8281 agttcgcatt cttcccgttg gcgccgttgc cgacctggaa cggtcccatg aactggaaga
     8341 aattcgcata gtcgttgctg ttgccggtat tttccagctc gcggccgttg ctgatcaggt
     8401 tgtagtagcg ccacgacgcg ttggaaccgg tcgtctgctg gaatgcgacg tccaggtccc
     8461 aggtcgaggc gaacgcgccc gggccgccgc tgttgtcgta ttcggcgata ctgatcgtcc
     8521 cgaacagccg cgccgagccg tcgtcaaatt cgttgaacgt cacgttgtcg aaggcgaacg
     8581 tcacctcatg gttgacgttg ttgtcgaaga aaccgtccag gcgcaggcca tagttgggcg
     8641 gcgacagcgc gccggcgacg cctgtggccg tccaggagtt gaccagcgtg gcggcggtcg
     8701 cggcgtgcgc ggccagaagg agcgtcaggg cgagaacaca gcccatcagg ggggagcggc
     8761 gcatgagaag atgatccttt catcaagtat tcgtccgcag tgcttgggcc ccctttctgc
     8821 aatccgtgtg ccgaccgcga tgcttcacgc agcccatttc tccccaatcg ctatctcctt
     8881 cattttcagt tgatcacgaa atctcaaatc ggcgttgcag ttccctgacg atccgccggc
     8941 tgactccagt gaccaccccg atgcagccca aatgacccaa ggtcgcatcc cgttgcacat
     9001 caaccccaac tttcgatcgc catcgatatc gctatcgcct ctcgtatccc cccgtcccct
     9061 ccatcagggc agcccgcggc gcaccagcag cagcgagatc acgccgaacg ggatggccca
     9121 cgccagcgcg atcagatgca ggctggtccc ccgcggcagc cccaggcgcc acagccacac
     9181 gctccagacc aggaaggccc agaatgcggc gttcaccgcc agccaccgca cgagacgaga
     9241 tcgagggagc atcctgcgcg cctcctgcaa cggtcgtcgc cgaaccgaat ccgctgattc
     9301 gagtcacgcg gtccaatcga tgttgccagt gattcctccg ccgcggcact acgccgtgat
     9361 catcgtcccg atgccgcggt cggtgaacaa ttcaatcagc agcgcgtgcg gcagccgccc
     9421 gtccaggatg tgcgccgagg caacgccggc ggcaatcgca tcgcggcacg cgcgcacctt
     9481 ggggatcatt ccccccttga ttaccccctc gcggatcagc ggctcgatct cctcgaccgc
     9541 gacgcggttg agcagcgtgg ccggatcccg cgggtcgcgg cagatgccgc gcacatcggt
     9601 cagcagaatc agtttgcgcg cctgcagcgc catcgcgcac tgcgcggcga cggtgtcggc
     9661 gttgatattg taggtctggc cgtcggccgc cgcgccgatc ggcgagatca ccggaatgta
     9721 gttcgtccgc tccagaatgc gcagaatctc gacattgacc tgctcgacct cgcccacgtc
     9781 cccgggagct gcgcggtggg ctttcttgat gt
//
LOCUS       DAOPCO010000148         9697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6695_length_9697_cov_4.963700, whole genome shotgun sequence.
ACCESSION   DAOPCO010000148 DAOPCO010000000
VERSION     DAOPCO010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6695_length_9697_cov_4.963700"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1104
                     /locus_tag="PLS90_10815"
     CDS             376..1104
                     /locus_tag="PLS90_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02935.1"
                     /translation="MPPLISQLIPRASRLALVVALGLAGCAKNEPVAVDSRAGSVELW
                     LGSPVVTTLGPLEGPYQLDRVETPRFIHLFDAAMKSTPFDVLGLRDDEYPMTNEQIKE
                     LGLGAEGLQQRRLDLRLYMVEQIQALLDKKELWVLRMAETQPPQVYLFHPRDKTAAGA
                     IPTGAAKLVNAWVLREGIAVMELASAIHPFYEKMLDAQLAAIGEARRYVADPAAARDV
                     WNRFRLNGPGPQFDARIELLAKPL"
     gene            complement(1109..1552)
                     /locus_tag="PLS90_10820"
     CDS             complement(1109..1552)
                     /locus_tag="PLS90_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02936.1"
                     /translation="MNPLRFIREHIWSMLPEVITDKQRLWAAVLWGDLLLGFGLWRGW
                     DALAAGRPPGAAALALVVGGFALQVLLLIPPVGRAIYRAILRVFSIVGFVLSSILLTV
                     FFYLVVTPMGLGLRLMGKDLLDRAGPPAWKPHTGRTDRRRYYRLF"
     gene            complement(1557..3002)
                     /locus_tag="PLS90_10825"
     CDS             complement(1557..3002)
                     /locus_tag="PLS90_10825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK02937.1"
                     /translation="MSASPPATPCRLLVIEDDPDYAQLLSRNLERSGFEATTVKSAEA
                     AFELLADRTFDLILTDIRLPRASGIDLIERVTAQIAAGEMEPQPIVVLTSINSVDMAV
                     EAMRKGAADYLTKEATRDEIALRLRKILEANELAHENRQLRRTLTRYEEFENIIGVSD
                     AIKRVKDQVAEIAASDVTVLIQGETGVGKELVARALHRASSRARGPFIEVNCAALPDE
                     NLLLSELFGHERGAFTGAINRKRGHFELADGGTLFLDEVGELGPIAQARLLRATESFE
                     FTRLGGERPIHVNCRLVFATNKDLAAEVKEGRFREDLYYRINVYPIFVPPLRARPEDI
                     APLARFFAARFAEKHHLAAPSFSPGALRALRDNPWPGNIRELRNVIERLSIRFHGREI
                     GPEALHDLNLGAPTDTGAIVLPEGGIDLEQVEESLVRQALQRAEWNQRRAAGLLGISV
                     DRMNARVKKFGITHPSWRVHKGGRGAPEDAD"
     gene            complement(3123..4988)
                     /locus_tag="PLS90_10830"
     CDS             complement(3123..4988)
                     /locus_tag="PLS90_10830"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK02938.1"
                     /translation="MSILRSISGCHEGRQFMLHEQTRIGRAPDNEVSLNDHLVSRYHV
                     LISRKGIHFDLRDLGSKNGVRVNNQPTMERRLCVGDQIRVGRTVFVFEVPREQKAARY
                     SDSLIHLDAYTDRQMQIFERADLACEADADWPTGDLVARLARLLQSENEDLPAMLEQL
                     LAGMIAQFRADSGALLLRTSSGDATPLITAGGIASLRISREAARLVLKEGKALIMALL
                     REQDGEIRGRSRRALMLPLMRREQVIGALQLQRAEGAEFTRDDLARAMALAGLATGAV
                     RFVIQMDQFILSGNETPSDPFIGHSQAAERVRERIRLMAANDSTTLLTGETGTGKELV
                     AKSIHQASARASMRFVAIDCSAIPANLLESELFGHEAGAFTGADRLKRGKVEMADGGT
                     LFLDEIGELQIELQPKLLRFLEERLFYRVGGTKPISADVRIIAATNRDLEKEVEAGRF
                     RQDLLYRLNVMPLQLPPLRTRLEDVRLLVEHFAPRLAARLGKPFLGLVDEAWVVLERY
                     SWPGNVRELRHGLERALILSDDGILRPEHFQLQIPEEPPEPTTDGLRTGARDSTVVDG
                     HDIRPQTLAEAEAEAIRRALRFAGGNRIQAAQHLDIHRNTLAKKITQYGIDLEKA"
     gene            5280..6035
                     /locus_tag="PLS90_10835"
     CDS             5280..6035
                     /locus_tag="PLS90_10835"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPK02939.1"
                     /translation="MPVRWIIADVDGCLSPEDSVAWDLPPFLRLVETVRRANAGESDI
                     APLTLCTGRPQPYVEVLMKLFDVRAPAIVENGAVFYTLPDNTSRYGPGVTEDKLLGLR
                     AVRAYIETDLLARFPEAVLQFGKEAQLSLFSRRTEQFAEMQRRIEAFVAERGGPDLLI
                     NASHFYLNISLRGVNKGNALRALMGELGVGHDDLAGIGDTVGDLPLREVVAFFACPAN
                     SQDEIKRVADYVSPFPFAEGVLDILRRPELRRR"
     gene            complement(6082..8880)
                     /locus_tag="PLS90_10840"
     CDS             complement(6082..8880)
                     /locus_tag="PLS90_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02940.1"
                     /translation="MVKSLFLSGRARRPLWLGSLLGRDWWFVPILVAAVVVRWGGLDK
                     SVWEDEVISFTEYETKGWVDPHLTSLGIFWVCNSLIETGSMRLVRLPVLLIGLFAIWL
                     GYALGRVWRRWLLGLLLATALAVWPFKAQWDTQVRYYAIMLTLMLAALVALEWYRARP
                     TTARWALALSVSVLGALTHILALPVIALPWVYLAWVLFTRGVRGIWRMVLGRTEPGWW
                     RLPLVLLVHLALLALATGATFQVSRGESVGGRVAALLERLEQRPDELDSGEGQAADRP
                     ARPTARRTGQAQVPTAGQAWQAATEMVRRAYQRDALSPNELFTRHPGTLRVTPAVTMS
                     RAWPGDFAPHRPWTPWLMYGLAAGCVALLFLSPPMLAGLLIVFFGTNILLDANVHLHG
                     LNPRYYVLPALAGLLVLIQAPVELFHWLRWPLHRRRPRWNAALVLLTGLICVPVLFWI
                     SWRHTRAAAGYIIQDWKQLYSVSRQQYPEGILFAGIVSKGIEFYNRLDRGGYDEQTPA
                     DYWRSPWRNEVVDAFKHPETVDMRLADLMAEQSAVVYFNPTYWRYDYEPIRTLGKLFE
                     PRPRDVFRVKSQHFDLQLKVIVPKGRLILPLAPRFQLNRELPADAPREYVMQLFFEVP
                     GSYEVQLGESPACRPLQASFGNEPLALRLAPARPWPPGRGVPLPTEEEVAAEAAADPQ
                     LNPLADGAGSVPATATARPPYPAAEDPDTTPTYVLPRDWRAAPGRRPPIYTHARRAYR
                     AEVRTPSTAMQRVLFSVTLDRDPGEFTPVVTFRRTDEEFVPVTEGFDIGSLVTWEEDG
                     DLLVSAPLRMHPNPDPEKERYAFGARIYLVDDTPGQRKEIHAWHTPLNDWVRDYQEGD
                     LAWLEPARITPAMGRQHAGNHLVLTLHPIVEVASAPGGVQALPLINEPRVYRPLNQFY
                     LIGLRYTEQDGHGQFVFD"
     gene            9161..>9697
                     /locus_tag="PLS90_10845"
     CDS             9161..>9697
                     /locus_tag="PLS90_10845"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPK02941.1"
                     /translation="MTPTLTSIELPGLNHVSSGKVREIFALNDELLLVATDRLSAFDV
                     VFNEPIPGKGRVLTQLAAFWFEKTRKIVSNHLLTTNLAEMPEPVRGRPELAGRTTLCR
                     RARTIPVECVVRGYLEGSAWKEYQQGQAVCGIKLPPGLKRRSRLPEPLFTPATKAEHG
                     LHDENITFEHCAETVGAET"
BASE COUNT         1642 a         3327 c         3188 g         1540 t
ORIGIN      
        1 agatccggcg aggcctggat catgcgaatc ggccgttcac cccccccata ttcatccctc
       61 gttgtgaacc gcaggttcaa gagcacttcg taatcgcgcc aatggccttt cccaattcgg
      121 ctctcggctc acgtcgctcg tcctcggccc tcgaatcttt gttatcgaac caataaggct
      181 gccgacaatt ccagcatgct tgtccttttc gtcccttccc atcgaccttt aaccctaaca
      241 cagatcctcc cgcccgtccc cggcaatggc cccggtggcg cgattcgcgc gatctcccga
      301 ttgacagcgc cgcggcggcg ggtaacttat cattgcgtgc tccttcgggc gcgagtttcc
      361 cttcggcggt ccatcatgcc cccgctcatc tcccagctca taccccgcgc atcgcggctg
      421 gcgctggtcg tggcgctcgg actagccggc tgcgccaaaa atgaaccggt cgccgtcgac
      481 agccgtgccg ggtcggtcga actctggctc ggcagcccgg tcgtgacgac gctcggcccg
      541 ctggaaggcc cctaccagct tgaccgcgtc gagacccccc gcttcatcca tctgttcgac
      601 gccgcgatga agagcacgcc gttcgacgtg ctgggcttgc gcgacgacga ataccccatg
      661 accaacgagc agatcaagga actggggctg ggcgccgaag ggctccagca gcggcgcctc
      721 gacctgcggc tttacatggt cgagcagatc caggccctgc tcgacaaaaa agagctctgg
      781 gtgctgcgca tggccgagac ccagccgccg caagtctacc tgttccatcc ccgtgacaag
      841 accgccgcgg gcgccattcc caccggcgcg gccaaactgg tcaacgcgtg ggtcctgcgc
      901 gaagggatcg cggtcatgga actggccagc gcgatccatc ccttttacga aaagatgctc
      961 gacgcgcagt tggcggccat cggcgaggca cggcgctacg tggccgaccc cgccgcggcg
     1021 cgcgacgtct ggaaccggtt ccgcctgaac ggccccggcc cgcagttcga cgcgcgcatt
     1081 gagctgctgg ccaaaccgtt gtaagccttc agaacaaacg gtagtaacgc ctgcggtcgg
     1141 tccggccggt gtgcggcttc cacgccggcg gaccggcgcg gtcgagcagg tccttgccca
     1201 tcagccgcag cccaagcccc atcggcgtca ccaccagata aaagaacacc gtcagcagga
     1261 tcgagctgag gacgaacccg acaatcgaaa agacgcggag gatggcgcga taaatcgcgc
     1321 ggccaaccgg cgggatcagc agcaggacct gcagcgcgaa gccgccgacg acgagcgcga
     1381 gcgccgccgc tccgggcggc cggccggcgg cgagggcgtc ccatccccgc cacaggccga
     1441 accccagcag caggtcgccc cagagcacgg cggcccagag ccgctgcttg tcggtaatca
     1501 cctcgggcag catgctccag atgtgttcgc ggatgaagcg cagcgggttc atggtgctaa
     1561 tcggcatcct ccggcgcgcc gcggccgccc ttgtgaacgc gccagctcgg atgcgtgatg
     1621 ccgaatttct tcacacgggc gttcatccgg tccacgctga tgccgagcag gccggcggcc
     1681 cggcgctggt tccactccgc ccgctgcagc gcctggcgca cgagcgactc ctccacctgt
     1741 tccaggtcaa tccctccctc gggcagcacg atggcgccgg tatcggtcgg cgcgccgagg
     1801 ttcaggtcat gcagcgcctc cgggccgatc tcgcggccgt ggaagcggat cgagagccgt
     1861 tcgatcacgt tgcgcagttc gcggatgttg cccggccagg ggttgtcgcg cagggcgcgc
     1921 agcgcgccgg ggctgaagct gggggcggcc aggtgatgct tctcggcgaa gcgcgccgcg
     1981 aagaaccgcg ccagcggcgc gatatcctcc gggcgcgcgc gcagcggcgg aacgaagatc
     2041 gggtagacgt tgatccgata gtagagatcc tcgcggaagc ggccttcctt gacctccgcg
     2101 gccagatcct tgttggtggc gaagaccagc cggcagttga cgtggatcgg ccgctcgccc
     2161 cccagccgcg tgaactcgaa cgattcggtc gcgcgcagca gccgcgcctg ggcgatcggc
     2221 cccagctcgc cgacttcgtc cagaaacagc gtgccgccgt cggcgagctc aaagtggccg
     2281 cgcttgcggt tgatcgcgcc ggtgaacgcg ccgcgctcgt ggccgaacag ctccgacaat
     2341 aggaggtttt catcgggcag ggcggcgcag ttgacctcga tgaacggccc ccgcgcgcgc
     2401 gacgaggcgc gatgcagcgc gcgcgccacc agctccttgc caaccccggt ctcgccctgg
     2461 atcagcacgg tgacgtcgct cgccgcgatc tcggcgacct gatccttgac gcgcttgatc
     2521 gcgtcggaga cgccgatgat gttctcgaat tcctcgtagc gggtcagcgt ccggcgcagc
     2581 tggcggttct cgtgcgccag ctcgttcgct tccaggatct tgcgcagccg cagcgcgatc
     2641 tcgtcgcgcg tcgcctcctt cgtcaggtag tcggcggccc ccttgcgcat ggcctcgacg
     2701 gccatgtcga ccgagttgat tgacgtgagc acgacgatcg gctggggctc catctcgccg
     2761 gcggcgatct gcgcagtgac ccgctcaatc aggtcgatgc cgctggcgcg cggcagccgg
     2821 atgtcggtca ggatcaggtc gaacgtccgg tcggcgagca gttcgaaggc cgcttcggcc
     2881 gatttgaccg tcgtcgcctc gaagccgctg cgctccagat tgcggctgag caactgggca
     2941 tagtccggat cgtcctcgat gaccagcagg cggcagggag ttgcgggcgg tgaagcagac
     3001 atggaccggg agacctccgc cccggcggct gcgcgcgccg cgcgggacaa ggcctatcat
     3061 tgaagcgggg gccccgccgc aatgcaaacg gaatcaagga aagccggccg gaggcccccc
     3121 gctcacgcct tttccagatc gatgccgtat tgggtgatct tcttggccag cgtgttgcgg
     3181 tggatatcca ggtgctgggc cgcctgaatg cggttgccgc cggcgaagcg cagcgcccgg
     3241 cggatcgcct cggcctcggc ttcggccagc gtctgggggc ggatgtcgtg gccgtcgacg
     3301 acggtcgaat cgcgcgcccc ggtgcgcagc ccgtcggtgg tcggttcggg cggctcctcg
     3361 gggatctgca actggaaatg ctccgggcgc aggatgccgt cgtccgacag gatcagcgcc
     3421 cgctccagcc cgtggcgcag ttcccgcaca ttgcccggcc aactgtagcg ttccagcacg
     3481 acccaggctt cgtccacgag gcccagaaac ggcttgccca gccgcgccgc cagccgcggg
     3541 gcgaagtgct cgacgagcag gcggacatcc tccagccgcg tgcgcagcgg gggcagctgc
     3601 agcggcatca cgttgagccg gtagagcaga tcctggcgga agcgccccgc ctcgacctcc
     3661 ttctccagat cgcgattggt ggcggcgatg atccgcacat cggccgagat cggcttggtg
     3721 ccgccgacgc ggtagaacag gcgctcctcc aggaagcgga gcagcttcgg ctgcagttcg
     3781 atctgcaatt caccgatttc gtcaagaaac agcgtgccgc cgtcggccat ctcgaccttg
     3841 ccgcgcttga gccggtccgc gccggtgaac gcccccgcct cgtgcccgaa caactcgctt
     3901 tccagcagat tggccgggat cgccgagcag tcgatcgcca cgaaccgcat ggaggcgcgc
     3961 gcgctcgcct ggtggatcga cttggcgacc agttccttgc ccgtcccggt ctcgccggtc
     4021 agcagcgtcg tgctgtcgtt ggcggccatc aggcgaatgc gttcacgcac gcgctcggcc
     4081 gcctgggagt gcccgatgaa cggatcgctg ggcgtctcgt tgcccgagag gatgaactga
     4141 tccatctgga tcacgaaccg gacggcgccg gtggcgaggc cggccagcgc catcgcgcgc
     4201 gcgaggtcgt cgcgcgtgaa ttccgcccct tcggcgcgct gaagctgcaa cgcgccgatc
     4261 acctgctcgc gccgcatcag cggcagcatc agcgcccggc gcgaacggcc gcgaatctcg
     4321 ccgtcctgct cgcgcaacag cgccatgatc agcgccttgc cttccttcag gaccagtcgg
     4381 gccgcctcgc ggctgatgcg cagcgatgcg atgcccccgg ccgtgatcag cggagtggcg
     4441 tcgcccgaac tggtccgcag cagcagggcg ccggaatcgg cgcggaattg cgcaatcatc
     4501 cccgcaagca gttgctcgag catcgcgggc aaatcctcgt tctcgctctg gagcaatcgc
     4561 gcgagccggg ccaccagatc ccccgtcggc cagtcggcgt cggcctcgca cgccaggtcg
     4621 gcccgctcga agatctgcat ctggcggtcg gtgtaggcgt ccagatggat cagtgaatcg
     4681 gaatagcgcg ccgccttctg ttcgcgcggc acctcgaaga caaacaccgt tcggccgacg
     4741 cggatctgat cgccgacgca caaccggcgt tccatagtcg gttgattgtt gacccgcacg
     4801 ccgttcttgc tgcccaggtc gcgcagatcg aaatggattc ccttgcgcga gatcagcacg
     4861 tggtagcgcg acacgaggtg atcgttcagg ctgacctcgt tgtccggcgc gcggccgatc
     4921 cgcgtctgct catggagcat gaactgacgc ccttcgtgac agccgctgat cgagcgcaag
     4981 atgctcacgt catctctccc ctcgcgggcc cccggcgcgc atccccaatc tagaggaggt
     5041 ctccgtccgg ggtcaagcgg ccccagcccg gcgccttcgt ccctcactca agaatccgtc
     5101 ctcgtctctc gtcctcgacc ttgtgctcga ctcccgaaat ccgtcggatc ggtccgatcc
     5161 gaccgatcca acggatttca aacatcctgg gtgacgcctt ccgggggggc gcttgcgcgg
     5221 gcgatgccaa gccgctataa aggggaccgc caccgcccgg gaggagagtg caacccgcca
     5281 tgccggttcg ctggatcatc gccgatgtcg atggctgcct cagccccgag gattccgttg
     5341 cctgggactt gcccccgttc ctgcgcctgg ttgaaacggt ccggcgcgcc aatgccggcg
     5401 agagcgatat cgcgccgcta acgctatgca ccggccgccc gcagccgtac gtcgaggtcc
     5461 tgatgaagct gttcgacgtg cgcgcgccgg ccatcgtcga gaacggcgcc gtgttctaca
     5521 cgctgcccga caataccagc cgctatggcc ccggcgtgac ggaggacaaa ctgctcgggc
     5581 tgcgcgcggt ccgggcctat atcgagaccg acctgctggc gcgcttcccc gaggccgtgc
     5641 tgcagttcgg caaggaagcg cagctctcgc tcttctcgcg ccggacggag cagttcgccg
     5701 agatgcagcg ccggatcgag gcgttcgtcg cggagcgcgg gggacccgac ctgctgatca
     5761 acgcctcgca tttctacctg aacatctcgc tgcgcggggt gaacaagggc aatgccctgc
     5821 gcgcgctgat gggggagctc ggcgtcggcc acgacgacct ggcggggatc ggcgacaccg
     5881 tcggcgacct tcccctgcgc gaggtggtcg cgtttttcgc ctgtcccgcc aattcgcagg
     5941 acgagatcaa acgcgtggcc gactacgtct cgccctttcc cttcgccgaa ggggtgctcg
     6001 acatcctgcg ccggccggaa ttgcgccgcc ggtgatcatc accatccatc tcccttcaat
     6061 gtccgtgggc gtccgtccgg cctaatcaaa gacaaactgg ccgtggccgt cctgctcggt
     6121 atagcgcagg ccgatcaggt agaactggtt cagcggtcga tagacccgcg gctcgttgat
     6181 cagcggcaag gcctggacgc cgccgggggc cgaggcgacc tcgacaatcg ggtgcagcgt
     6241 gagcaccaga tgattgccgg catgctggcg tcccatggcc ggcgtgatcc gggccggctc
     6301 cagccaggcc aggtcgccct cctggtagtc gcgcacccag tcgttgagcg gggtgtgcca
     6361 ggcatggatt tccttgcgct ggcccggcgt gtcgtccacc aggtagatcc gcgcgccgaa
     6421 ggcatagcgt tccttctcag gatccggatt gggatgcatc cgcagcggtg cgctgacgag
     6481 caggtcgcca tcctcctccc aggtgacgag cgacccgata tcgaagccct cggtcacggg
     6541 gacgaattcc tcgtccgtgc ggcggaaggt aacgacaggg gtgaactcgc ccgggtcgcg
     6601 gtcgagcgtg acggaaaaca ggacgcgctg catggcggtg gagggcgtgc gcacttcggc
     6661 gcgataggcg cgccgcgcat gggtgtaaat cggcggtcgc cggcccggtg cggcgcgcca
     6721 gtcacgcgga aggacgtagg tgggggtggt gtccgggtcc tctgccgcgg ggtacggcgg
     6781 ccgagccgtc gcggtggccg gaaccgaacc cgcgccgtct gcaagcgggt tgagctgcgg
     6841 atcggccgct gcttcggcgg ccacctcctc ctcggtcggc agcgggacgc cacggcccgg
     6901 cggccagggg cgcgcggggg ccaggcgcag cgccagcggc tcgttcccga agcttgcctg
     6961 cagcggacgg caggcggggg actcgccgag ctggacttca tagctgccgg ggacttcaaa
     7021 gaagagctgc atcacgtatt cgcggggggc gtcggccggc agttcgcgat tcaattgaaa
     7081 acgcggcgcc agcggcagga tcaaccgccc cttggggacg atcaccttga gctgcaagtc
     7141 gaagtgctgc gatttgacgc ggaacacatc gcgcggccgc ggctcaaaga gtttccccaa
     7201 cgtgcggatc ggctcatagt catagcgcca gtacgtgggg ttgaaataga cgacggccga
     7261 ttgctcggcc atcaggtcgg ccagtcgcat gtcgaccgtc tcgggatgct tgaaggcgtc
     7321 gacgacctca ttgcgccagg gcgaccgcca gtaatccgcc ggcgtctgtt cgtcgtagcc
     7381 gccgcggtcc agccggttgt agaactcgat ccctttggaa acgatcccgg cgaacaggat
     7441 gccctcggga tactgctggc gcgagacgga gtaaagctgt ttccaatcct gaatgatgta
     7501 tccggcggcg gctctcgtgt gccgccaact gatccagaag agcacgggaa cgcagatcag
     7561 ccccgtcagc agcaccaggg cagcattcca gcgcggccga cggcggtgga gcggccagcg
     7621 cagccagtgg aacagctcca ccggcgcctg gatcagaacc agcagcccgg ccagggccgg
     7681 cagcacgtaa tagcgcggat tgaggccgtg cagatggaca ttggcatcga gcaggatgtt
     7741 ggttccgaaa aagacgatga gcaaaccggc cagcatcggg ggtgagagga acaggagcgc
     7801 gacacagccg gcggccaggc cgtacatcag ccagggggtc catggccggt gcggcgcgaa
     7861 atcgcccggc cacgcccgac tcatcgtcac ggcgggcgtc acccgaagcg tccccgggtg
     7921 acgggtaaag agctcgttgg ggctgagcgc atcacgctga tacgcccgcc ggaccatctc
     7981 ggtcgccgcc tgccacgcct ggcccgcggt cggcacctgg gcctggccgg tgcggcgcgc
     8041 cgtggggcgg gccggccggt cggcggcctg cccctcgccc gaatccagtt cgtcggggcg
     8101 ctgctccagt cgttcgagca gcgccgcgac ccgtccgccc accgattcgc cgcggctgac
     8161 ctgaaacgtt gcccccgtcg ccagcgccag cagggccaga tgcaccagca gcaccagcgg
     8221 caggcgccac cagcctggct cggtccggcc cagcaccatc cgccagatcc cgcgcacccc
     8281 ccgggtgaac aggacccagg ccagataaac ccacggcagc gcgatgaccg gcagggccag
     8341 gatatgcgtc aacgccccca ggacggaaac cgacagcgcc agcgcccagc gggccgtcgt
     8401 cgggcgcgcg cgataccact ccagcgccac cagcgccgcc agcatcagcg tcagcatgat
     8461 ggcgtaatag cgcacctgcg tatcccactg agccttgaac ggccagacgg ccagcgccgt
     8521 cgcgagcagc agccccagca gccagcggcg ccagacgcgc cccagtgcat atcccagcca
     8581 gatggcgaag agcccgatca gcaggaccgg cagccggacc agccgcatcg agcctgtttc
     8641 gatcagggaa ttacagaccc agaaaatccc cagcgacgtg aggtggggat cgacccagcc
     8701 cttggtttca tactcggtga aggagatgac ctcgtcctcc cagaccgact tgtccagccc
     8761 gccccagcgg acgacgacgg cggcgacgag aatggggaca aaccaccagt cgcggccgag
     8821 cagggatcca agccagagcg gtcggcgtgc acggccggac agaaacagcg acttcaccat
     8881 gggggaacaa ctatcattga ttcggttggg ggacagcgaa aacgcaaacc acgctccaca
     8941 acaatctttc tagttggggg gcatttggct ggcgtttgca agcaaaaacg aaagattgtg
     9001 taaaatgtaa acacttgacg atcaggcccc gccaccggct ttggcgttta cggccgacct
     9061 ggattgtgcc acattggctc gaggaccggc ctccgggtcc ccatgtccgg gcaggccgtg
     9121 ccggtcccgc atcgtccgtt gctacaccgg caggcaaacc atgacaccca cgctgacttc
     9181 cattgagttg ccggggttga accatgtctc ctccggcaag gtccgcgaaa tctttgcgct
     9241 gaacgacgaa ctgctgctcg tcgccaccga ccgtctgagc gcgttcgatg tggtgttcaa
     9301 cgaaccaatt ccgggcaagg gccgcgtgct gacgcaactg gcggccttct ggttcgagaa
     9361 gacgcgcaag atcgtcagca atcacctgct cacgaccaac ctggcggaga tgcccgagcc
     9421 ggtccgcggc cggccggagc tggccgggcg cacgacgctc tgccgccgcg cccggacgat
     9481 tccggtcgaa tgcgtcgtcc gcggctatct ggagggctcg gcctggaaag agtatcagca
     9541 gggccaggcg gtctgcggaa tcaagctgcc gccggggttg aagcgccgtt cccgcctgcc
     9601 cgagccgctg ttcacgccgg cgaccaaggc cgagcacggc ctgcatgacg agaacatcac
     9661 cttcgagcac tgcgccgaga ccgtcggcgc cgagacc
//
LOCUS       DAOPCO010000149         9664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6726_length_9664_cov_4.859403, whole genome shotgun sequence.
ACCESSION   DAOPCO010000149 DAOPCO010000000
VERSION     DAOPCO010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6726_length_9664_cov_4.859403"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLS90_10850"
     CDS             complement(<1..418)
                     /locus_tag="PLS90_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK02942.1"
                     /translation="MAMIEIRRMRKAFGSHVVLEDVDLDIERGETHIILGRSGTGKSV
                     LLKIICGLIEPDGGRLVVDGDVKGEVDKKQWRAAMAKVQMLFQGAALFDSMTIEQNVA
                     FHAIEHGEIKRSQASAYAREFLEMVDLADAGGKMPAE"
     gene            complement(562..2268)
                     /locus_tag="PLS90_10855"
     CDS             complement(562..2268)
                     /locus_tag="PLS90_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02943.1"
                     /translation="MTSPAPAAGPPPGEPAPDPVPSAPRCRVWPLLLAIVVLAGMVRC
                     LGLGNWPPGLFRDEAEKGYNAWALATTGGVVQFEPLPDQPPAPRFERLPFMISVVGTR
                     TSAIYQYASIPFVGLGGLSVATTRMAAAAAGTLAVLLLGALIARAWGAPAGLAVAFWL
                     ACSPWHLVFSRWALQGIFVPLLMAAVLWGLWLAERAPTRRQGLAGFPLAGFSLGMLYY
                     AYSGASPFALAWGACLLILHRREIGRRPRAALAGLLLFLAWFIPTTAVRLAPGGAERL
                     ARIAIWSDPGLSAAGLLLAFLRNYLAHFDPRFLFLTGDAQPRHGIPGTGVLLLADAVL
                     LPIGLVWTFRKRQPLAGALLAALLCAPIPAAITRVGIPHALRAIGMIVPAVAWSGAGL
                     VVLLGWLLHRFDAIGSGAPESHRRGRVYAGLLLLALGGLAFNGLWRYGLHARDPIMQV
                     AFQEGDRRAWQAAADRRGAMRLHIPYLSPFTLYHYLFYFQVPPHQAHRVFENDGPVQW
                     FNPEQEPVELVWQRMAPGEALMEWVDPAWLRDERGRPLLTPAESARAGEAWARLRLKP
                     GN"
     gene            complement(2265..3497)
                     /locus_tag="PLS90_10860"
     CDS             complement(2265..3497)
                     /locus_tag="PLS90_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPK02944.1"
                     /translation="MYTAFDFRLPQCILFGAGRFSEAATHAARFGGRALLVTGGQSLE
                     RTGRLAALEDQLRAAGVAWRRIVVRGEPTVSVVDQGAAEARRFQPDCVLSVGGGSVLD
                     AGKAIAALLTNPGETLDYLEGVGRGRTLDQPALPHIAVPTTAGTGSEATLNAVITDDQ
                     HAFKKSMRADGMLPTVALVDPDLLEGCPAETAAACGMDAVTQLIEAYVARNASPFTDA
                     FALQGLYHASRLADYLHDTTQPAARSSMALASLIGGICLANAGLCVVHSLASPLGAFF
                     DAPHGVICAALLPPSVQLNVERAQAEGNRELLDKYCMAYDLLLAPDSAHAAAPMTRGG
                     IVEALDFLNEPARGAQALALRLGELRQELNLPGLAHYGVTTADFGRIIDAAGTANLHN
                     NPVELDRAALTRLLELAL"
     gene            complement(3549..3869)
                     /locus_tag="PLS90_10865"
     CDS             complement(3549..3869)
                     /locus_tag="PLS90_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPK02945.1"
                     /translation="MFVVCVTVWVREAYRDAFIAATLENARNTRGEPGNLRFDVLQAV
                     DDPNRFFLYEVYVDEAAFAEHQQTAHYFAWRDTVADWMAQKRQGVKHRALDPADATAW
                     TAQA"
     gene            complement(3919..4701)
                     /locus_tag="PLS90_10870"
     CDS             complement(3919..4701)
                     /locus_tag="PLS90_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02946.1"
                     /translation="MANPWKIFGIGCLVIVVLIAIVMGIGFYMAFKFGSGLARQVQAA
                     QARVAELNAQFPFAADTGTLPDAERFETWLQVTRDAGAAMEQHGTDFEQRVESAAEEG
                     MFQALGAMGGLGDLVAGIFGSLGDALQSRQMSLDEYKWYTATAHTALRMQGATTDTTL
                     TEIARQVRQRQDADVSPPATPELSIAQIETMQRILTDAAAQHPDLAERIAGAINLLGM
                     LRQSHVGEMEAPAPPRPAELDAPPGSAPPSSPADAPLPAPAG"
     gene            complement(4752..5690)
                     /locus_tag="PLS90_10875"
     CDS             complement(4752..5690)
                     /locus_tag="PLS90_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPK02947.1"
                     /translation="MRWVTYNTGKPLRAGLLIDDLVVDFEQSARALLGRSAPASVRAI
                     LEWGNPGRQLAERLLKRTRKLVQRIEQGTERRPAWLIPLDRVHLNAPVPDPEKVICLG
                     LNYLEHCREQEGRLGRPVEPPKQPILFAKFASTLTGPYDPIVLPDPKVTREADYEVEL
                     AVVIGRTARGVSQRAAADHIAGYMVLDDVSARDCQREDHQWVRSKSFDTFAPCGPALV
                     TPDEVPNPGRLALTTRLNGEVMQSSNTSDMIFKIPRIISFISQAITLKPGDIITTGTP
                     PGVGAFREPPISLKPGDVVECEIEGLGVLRNVCRRG"
     gene            complement(5817..6305)
                     /locus_tag="PLS90_10880"
     CDS             complement(5817..6305)
                     /locus_tag="PLS90_10880"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPK02948.1"
                     /translation="MTDAAPPFEAWMDLALAEAEAALAHDEVPVGCVIADGSGAIVAR
                     AHDARQAAADPLAHAELLALRQAAAAQGDWRLDGMTLVVTLEPCPMCAGSILMARIAR
                     VVFGARSPKWGAAGSRADWLGSGVFPHRPEVIGGIREAPCGEVLSRYFRRLRKSADEM
                     GG"
     gene            complement(6616..9357)
                     /locus_tag="PLS90_10885"
     CDS             complement(6616..9357)
                     /locus_tag="PLS90_10885"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK02949.1"
                     /translation="MSSPFRVFVYALVAAVLAAPIAAVEPASTPPVVPLGLDAYLQWE
                     KWPLQRLGVRSYMRSTYDRSGGNQSADASHYLYQEAEDFNVALDELGPGVLYFKRTNH
                     WHGSPWHYEVDGTDHIVQESSTADPLNPVADSVFLPEAPFPNPLTWTWSVTRGADLMW
                     VPIMFEQQLRIAYSRTHYGTGYFILHKLLPGIALSRPLRSWSIQEIPDPAVLDLINRS
                     GTDIAPADTLIQEAGDLSLAAGGTLPVAQIQGPATLRALKFTLPLDRALDLERVRLRI
                     TWDGRAQPSVDAPLCLFFGAGTFYNRDGREWLVKAFPVSIRQTADELQLACYFPMPFF
                     REARIELADVPASEAEIGYEIRAEHYDGPSNHVGYFHATYRDHPDPEPGVDLVYLDTR
                     EVEGGGDWTGAFVGSSFIFSHHAILGTLEGDPRFFFDESQTPSYGTGTEEWGGGGDYW
                     GGRTMTLPFAGHPVGAPNPDAAHNDRDRIESAYRFLLADLMPFGKRAIIRNEHGALND
                     SREHYESVVYWYGLPGASLVRTDVLDVGDPADEAAHDYVSPDATAPYRFSSRYEWGPT
                     AVELTAPNRPEANPASYAEFSFHLPAAGEYSLWVHGRAERPFVTSDAFWVQFDGEIGT
                     PAFTAAANGINGLGNWLDGHPGELDYRWSSGMPNAVPQVMRFAEPGTHTLRIQPRHRG
                     HFLDEIRLTPVNAVPLNDQPADALAEAAVAAAAVVLRAEDARTLAGAYRLLDDRGARA
                     GKALYIPSETREVVPVQTKDGRRTDGTSEFTLRIDPDNHGVMLRRTLDYQYPNQRARI
                     WIAAVGEDGNPGDWREAGVWYEAGADTHLYSDPPGELDPTRKTLQTSGRRFRDSEFLI
                     PLELTRGQSAIRVRAEFWPLERSLWPGQEFPHRTTWTELEYQAWSYVMPPDPFANDD"
BASE COUNT         1634 a         3248 c         3320 g         1462 t
ORIGIN      
        1 gctccgccgg catctttccc cccgcgtccg ccaggtcgac catctccaga aattcgcgcg
       61 cgtaggcgct cgcctggctc cgcttgatct cgccgtgctc gatggcatgg aacgcgacgt
      121 tctgctcgat cgtcatcgag tcgaacagtg ccgccccctg gaataacatc tggaccttgg
      181 ccatcgcggc gcgccactgt ttcttgtcga cctcgccctt gacgtcgcca tcgacgacaa
      241 gccgcccgcc atcgggctcg atcagcccgc agatgatctt gagcaggacc gatttgccgg
      301 tgccggagcg gcccaggatg atgtgcgttt cgccgcgctc gatgtcgagg tcgacgtcct
      361 ccagaacgac gtgcgagccg aaggccttgc gcatccgccg gatttcaatc atcgccatgg
      421 ctgaggaggc ctcgatgcag gctgccgcgc ggccggcgcg cggggcggtt gtcggattct
      481 atagcgcgcc ggaaacgatt ggaagccccc gtgaggtgaa cggaactcga tgctcgccct
      541 gatcgcgccg tcggccaggc atcagttccc cggcttgagc cgcagccgcg cccaggcttc
      601 gccggcgcgc gccgactcgg cgggagtcag cagcggcctg ccccgttcgt cgcgcaacca
      661 ggccggatcg acccattcca tcagcgcctc gccgggcgcc atccgttgcc agacaagctc
      721 cacgggctcc tgttccggat tgaaccactg gaccggcccg tcgttttcga acacgcgatg
      781 cgcctggtgc ggaggaacct gaaaataaaa gagataatgg tagagcgtga agggagagag
      841 ataagggatg tgcaggcgca tcgcgccgcg ccgatcggcc gcggcctgcc aggcccgccg
      901 gtcgccttcc tggaaggcga cttgcatgat cgggtcgcgg gcgtgcaggc catagcgcca
      961 caaaccattg aatgccaggc cgcccagcgc cagcagcagg agcccggcgt aaacccggcc
     1021 acgccggtgc gattcgggcg cgccggaccc gatggcgtcg aaccggtgga gaagccagcc
     1081 gagaaggaca accaggcccg cgccgctcca ggccacggcc gggacgatca tgccgatggc
     1141 gcgcagcgcg tgcgggatgc cgacgcgcgt gatcgccgcc gggatcggcg cgcacaatag
     1201 cgccgccagc agcgcccccg ccagcggctg ccgcttgcgg aacgtccaga caagcccgat
     1261 cggcagcagc acggcgtcgg cgagcaggag cacgccggtt cccgggatgc cgtggcgggg
     1321 ctgggcgtcg cccgtcagaa aaagaaaccg ggggtcgaag tgcgcgaggt agttgcgcag
     1381 aaaggccagc agcagcccgg cggcggagag gccggggtcc gaccagatgg cgatgcgcgc
     1441 caggcgttcg gcgccgcccg gcgccagccg gaccgcggtc gtggggatga accacgccag
     1501 gaacagcagc agcccggcca gcgcggcgcg cggccgccgg ccgatttcac gccgatggag
     1561 aatcagcagg cacgcccccc aggccagggc gaacggcgaa gcgccggaat aggcgtaata
     1621 cagcatgccg agcgaaaagc cggccagcgg gaagcccgcc agcccttgcc gtcgtgtggg
     1681 ggcccgctcc gccagccaca gaccccacag cacggccgcc atcagcaggg gaacgaaaat
     1741 gccctgcaac gcccaacggc tgaacaccag atgccacggg ctgcacgcca gccagaacgc
     1801 gacggcgagg ccggcgggcg cgccccaggc gcgggcgatg agcgcgccga gcaacaggac
     1861 ggcgagcgtt ccggcggcgg ccgcggccat ccgggtcgtg gcgaccgaca ggccgcccag
     1921 accgacaaac gggatcgagg cgtactggta gatggccgag gtgcgcgtgc cgacgactga
     1981 gatcatgaac ggcagccgtt cgaaccgcgg cgccggcggc tggtcgggga gcggctcaaa
     2041 ctgcaccacc ccgccggtcg tggcaagcgc ccaggcgttg tagccttttt cggcttcatc
     2101 gcggaacagg ccgggcggcc agttacccaa cccaagacag cggaccatgc ccgccagcac
     2161 cacgatggcc agcagcagcg gccagacccg gcagcgcggc gccgaaggaa cggggtcggg
     2221 cgccggttcg cccggcggtg ggccggcggc cggggcaggg gaggtcaaag cgccagctcc
     2281 agcagccggg tcagggcggc gcggtcgagc tcgacgggat tgttgtgcag attggccgtg
     2341 ccggcggcat cgatgatccg cccgaagtcg gcggtcgtga cgccatagtg cgccaggccg
     2401 gggaggttga gttcctggcg cagctcgccg agccggaggg ccagcgcctg cgcgccgcgc
     2461 gccggctcat tcagaaaatc gagcgcctcg acaatcccgc cgcgggtcat cggcgccgcg
     2521 gcatgggccg aatccggggc cagcagcaga tcataggcca tgcagtactt gtcgagcagc
     2581 tcgcggttgc cttcggcctg ggcgcgctcg acattgagct gaaccgacgg gggaagcagc
     2641 gccgcgcaga tgacgccgtg cggggcatca aaaaacgcgc ccagcggcga ggccaggctg
     2701 tgcaccacgc agagcccggc gttggccaga cagatgccgc cgatcaggct ggccagcgcc
     2761 atcgacgagc gcgccgcggg ctgcgtcgta tcgtgaaggt agtccgccag ccggctggca
     2821 tgatacagcc cctgcagcgc gaaggcgtcg gtgaacgggc tcgcgttgcg cgccacgtag
     2881 gcctcgatca actgggtgac ggcgtccatg ccgcacgcgg cggcggtctc ggcggggcag
     2941 ccttccagca gatcggggtc gaccagcgcg acggtgggga gcatgccgtc ggcgcgcatc
     3001 gatttcttga aggcgtgctg gtcatcggtg atgacggcgt tgagtgtcgc ctccgagccg
     3061 gtgccggcgg ttgtcggaac ggcgatgtgc ggcagggccg gctggtcgag cgtccggccg
     3121 cgcccgaccc cctccagata gtccagcgtt tcgcccggat tggtcagcag cgcggcgatg
     3181 gccttgccgg catcgagcac gctgccgccc ccgaccgaca ggacgcagtc gggttgaaac
     3241 cggcgggctt cggcggcgcc ctgatcgacc accgaaacgg tcggttcgcc gcgcaccacg
     3301 attcgccgcc aggcgacgcc cgccgcgcgc agttggtcct caagcgcggc gagccggccg
     3361 gtccgctcaa gcgactggcc gccggtcacc agcagcgccc gcccgccgaa gcgcgcggcg
     3421 tgcgtcgccg cttcggagaa ccggcccgcg ccgaacagga tgcactgtgg aaggcgaaag
     3481 tcgaaagcag tgtacatggg tcgcgcctcg ttgcgccggg gcgaaaaccc gtcgcccggg
     3541 agtcgggatc aagcctgggc ggtccaggcc gttgcgtctg cgggatccag cgcccggtgc
     3601 ttcacgccct ggcgcttctg ggccatccag tcggccacgg tgtcgcgcca cgcaaagtag
     3661 tgcgccgttt gctggtgctc ggcgaatgcc gcctcatcca catagacttc gtagaggaag
     3721 aagcggttgg gatcatcgac cgcctggagg acgtcgaagc gcaggttgcc cggctcgccg
     3781 cgcgtgttgc gggcattctc gagggttgcg gcgatgaacg cgtcgcggta cgcttcccgc
     3841 acccagaccg tcacgcaaac gacaaacatc gtgctcgcct cctttcaggc gtggattgaa
     3901 cgtgaactgc tggaacgctc agccggccgg cgcgggcagc ggggcatccg cgggagacga
     3961 tggcggggcc gaacccggcg gcgcatcaag ctccgccggc cgcggcggcg cgggcgcctc
     4021 catctcgcca acgtgagact ggcgcaacat gccgaggagg ttgatcgccc cggcgattcg
     4081 ttcggccaga tcgggatgct gcgccgcagc gtcggtcagg atccgctgca tcgtttcgat
     4141 ctgggcgatc gacagctccg gcgtcgccgg gggggaaaca tccgcgtcct gccgctggcg
     4201 cacttgccgc gcgatctcgg tcagcgtcgt gtcggtcgtg gccccctgca tgcgcagcgc
     4261 cgtgtgcgcc gtcgcggtgt accacttata ctcgtcgagc gacatctggc ggctttgcag
     4321 cgcgtcgccc agcgagccga aaatgccggc gaccagatcg ccgagaccgc ccatcgcacc
     4381 cagcgcctgg aacattccct cctcggccgc cgattcgacc cgttgctcaa agtcggtccc
     4441 gtgctgctcc atcgcggccc cggcatcgcg ggtcacctga agccaggtct cgaaccgctc
     4501 cgcgtcggga agggtgccgg tgtccgcggc aaaggggaat tgcgcattca gttcggcgac
     4561 gcgcgcctgg gccgcctgca cctgccgggc caggcccgaa ccgaatttga acgccatgta
     4621 gaacccgatc cccattacga tcgcgatcag gacgacaatc accagacagc cgatcccgaa
     4681 aatcttccac ggattcgcca tggcgtgtcc ctttcaggtt ggggggccgg ggagcgcggc
     4741 gcgagaccgc gtcacccgcg tcggcagacg ttgcgcaaga cgcccaggcc ctcgatctcg
     4801 cattccacga catcgcccgg cttgagggag atcggcggct cgcgaaatgc ccccacgccc
     4861 ggcggcgttc cggtcgtgat gatatcgccc ggtttgagcg tgatcgcctg ggagataaat
     4921 gaaatgatcc gcgggatctt gaagatcatg tcgctggtgt tgcttgactg catcacctcg
     4981 ccgttgagcc gggtggtcag tgccagccgg cccggattgg gcacttcgtc gggggtcacc
     5041 agggcgggcc cgcatggcgc gaacgtatcg aagctcttgg agcggaccca ctggtggtcc
     5101 tcgcgctggc aatcgcgcgc cgacacatcg tccagcacca tgtagccggc gatatggtcg
     5161 gcggccgcgc gctgggagac gccgcgcgcc gtccggccga tcaccaccgc cagttcgact
     5221 tcatagtccg cctcgcgcgt caccttggga tcgggcagca cgatgggatc atagggcccc
     5281 gtcaacgttg aagcgaattt ggcgaacaga attggttgtt tcggcggctc gacgggccga
     5341 cccagtcgac cctcctgctc gcggcagtgc tccaggtagt tcaggccgag gcagatgacc
     5401 ttctcggggt cgggcaccgg cgcgttgagg tggacccggt cgagcggaat cagccaggcg
     5461 ggacggcgct cggtcccctg ctcgatccgc tgcacgagct ttcgcgtccg tttgagcagc
     5521 cgctcggcca gttgccggcc cggattgccc cactccagga tcgcgcggac cgaagcgggc
     5581 gccgaccggc cgagcaacgc ccgggcgctc tgttcgaagt ccacaaccag atcatcgatc
     5641 aacaggcccg cgcgcagcgg cttgcctgtg ttgtaagtga cccatcgcat gcgcgagaca
     5701 tcgcctccct tcaggtggtt cgcgcaatcg taggatttcc gaggttaata gccaaccaaa
     5761 accgggggcc gcgcgtgcat cgcgaaatac ggcgaggttc acagtcgttg ccggcgctat
     5821 ccgcccatct cgtcggcgga ctttctcagt cgccgaaaat accggctcag cacttcgccg
     5881 cagggcgcct cccggatgcc gccgatcacc tcgggccgat gcgggaagac gcccgagccg
     5941 agccagtcgg cgcgcgagcc ggcggcgccc catttcgggc tgcgcgcgcc aaacaccacg
     6001 cgcgcgatcc gcgccatcag gattgacccg gcgcacatcg ggcacggctc cagcgtgacc
     6061 accagcgtca tgccgtccaa ccgccagtcg ccctgcgcgg cggccgcctg gcgcagcgcc
     6121 agcagttcgg cgtgggcgag cggatcggcc gccgcctgac gcgcgtcatg ggcgcgcgcg
     6181 acgattgccc ccgacccatc ggcaatcacg cagccaaccg gaacctcgtc gtgggcgagc
     6241 gccgcctcgg cctccgccag cgccagatcc atccaggctt cgaaaggcgg ggcggcgtcc
     6301 gtcatcccgc gatcctccgg cccgcggcgc gccgccggcc gcatggttcg atcaccggaa
     6361 tcaacggatg gaccgaacgg attcgcggcg gtattccccg gttgatcatc tccctgcctc
     6421 gggggcgcgt aatcccgttc ctcgcgcatc gagcccctga attcaacgac gttcgcccca
     6481 cgctaccggc cgcggcgcgc cgcatcaagg ccgatatagt tcaccgttcc cggttatctc
     6541 cggtttgccg gcggaaacca ccgggtacaa tcgccggcgt tgttgtcggc ccgaggagcg
     6601 aacgcgacgt ttcgttcaat cgtcgttcgc gaacgggtcc ggcggcatga cgtagctcca
     6661 ggcctggtac tccagttcgg tccaggtcgt ccggtgcgga aactcctggc cgggccagag
     6721 cgaacgctcg agcggccaga actcggcgcg gacgcggatg gcggactgcc cgcgcgtcag
     6781 ctcgagcggg atgagaaact cgctgtcgcg gaatcgccgc cccgacgttt gcagggtctt
     6841 gcgggtcgga tcgagttcgc cgggaggatc gctgtaaagg tgcgtatcgg cgcccgcctc
     6901 gtaccacacg cccgcttcac gccaatcgcc cgggttgcca tcctcgccca ccgccgcaat
     6961 ccagatgcgg gcgcgctgat tggggtactg gtaatcgagc gtgcgccgca gcatgacccc
     7021 gtgattgtcc ggatcgatgc ggagcgtgaa ttcggacgtt ccgtcggtcc ggcggccatc
     7081 cttggtctgc accggcacga cctcgcgcgt ttcgctgggg atgtagagcg ccttgccggc
     7141 gcgcgcgccc cggtcgtcga gcagccgata agcgccggcg agggtccgcg cgtcttcggc
     7201 gcgcagcacg accgccgccg cggcaacggc ggcttccgcc agcgcgtcag cgggttgatc
     7261 gttcagcggg acggcgttga ccggcgtcag gcggatctca tccagaaaat gcccacgatg
     7321 gcgcggctgg atccgcagcg tgtgggtgcc cggctccgcg aagcgcatga cctgcgggac
     7381 ggcgttgggc atcccgctgg accagcggta gtccagctcg ccgggatggc cgtcgagcca
     7441 gttgcccagc ccgttgatgc cgttggccgc cgccgtgaaa gccggcgtgc cgatctcacc
     7501 gtcgaattgc acccagaagg cgtcggacgt gacgaacggg cgctccgcgc ggccatgcac
     7561 ccacagcgag tattcacccg ccgcgggaag gtggaagctg aactcggcgt agctggcggg
     7621 gttggcctcg ggtcgattgg gcgccgtcag ctcaaccgcc gtcggtcccc attcgtaacg
     7681 tgaactgaat ctgtaaggcg cggtggcgtc cggcgagacg tagtcgtgcg cggcttcgtc
     7741 ggccggatcg ccgacatcga gcacatcggt ccggacgagc gatgcaccgg gcaggccata
     7801 ccagtagacc accgattcat agtgctcgcg cgaatcattc agcgcgccgt gttcattgcg
     7861 gataatcgcg cgcttgccga acggcatcag gtcggccagc agaaaccgat aggccgattc
     7921 aatccggtcg cggtcgttgt gggccgcgtc cggattcggc gcgccgaccg ggtggccggc
     7981 gaacggcaac gtcatcgtcc ggccgcccca gtagtcgccc ccgccgcccc attcctcggt
     8041 gccggtgccg taggaggggg tctgcgactc gtcgaagaag aaccgcgggt ccccctccag
     8101 cgtgccgagg atcgcatgat gggagaaaat gaacgagctg ccgacgaacg cgccggtcca
     8161 atcgccgccc ccctccacct cccgcgtgtc gagatagacc aggtcgactc cgggttcggg
     8221 atcgggatgg tcccgatagg tcgcgtgaaa atagccgaca tgattcgatg gcccgtcgta
     8281 gtgctccgcc cggatctcgt agccgatctc ggcctcgctc gcgggcacat ccgccagttc
     8341 gatgcgggct tccctgaaga acggcatcgg gaagtagcac gcgagctgga gctcgtccgc
     8401 tgtctgccgg atgctgacag gaaacgcctt gaccagccac tccctgccgt cgcgattgta
     8461 gaacgtgccg gcgccgaaga agagacacag cggcgcgtcg accgagggct gcgcgcgccc
     8521 gtcccaggtg atgcgcagcc ggacccgctc cagatccagg gcgcgatcca gcggtagcgt
     8581 gaacttgagc gcgcgcagcg tcgccggtcc ttggatttgc gcgacgggca gcgtgccgcc
     8641 ggcggcgagt gagaggtcgc cggcctcctg gatcagcgtg tcggcggggg cgatgtcggt
     8701 tccgctgcgg ttgatcagat ccagcacggc cggatccgga atctcctgga tcgaccagga
     8761 acgcagcggg cgtgaaagcg caatcccggg cagcagcttg tgcagaatga aatagcccgt
     8821 gccgtagtgc gtgcggctat aggcgatcct caactgctgc tcgaacatga tcggcaccca
     8881 catcaggtcg gccccccggg tcaccgacca ggtccaggtg agcgggttgg ggaagggtgc
     8941 ctccgggagg aagaccgagt cggcgaccgg attcagcgga tcggccgtgg agctctcctg
     9001 gacgatgtga tcggttccat ccacttcata gtgccacggg ctgccgtgcc agtgattggt
     9061 gcgcttgaaa tacaacacgc cggggcccag ttcatcgagc gcgacgttga aatcctcggc
     9121 ttcctgatac aggtaatggc tcgcatcggc gctctggtta ccgccggacc ggtcgtaggt
     9181 gctgcgcata taggagcgca cgcccagccg ttgcaggggc catttctccc actggaggta
     9241 ggcatcgagc ccgagcggca cgacgggcgg cgtcgaggcg ggctcgaccg cggcgatcgg
     9301 cgccgcgaga actgcggcga ccagggcata aacgaaaaca cgaaaaggag acgacatggc
     9361 tgaaactcaa cgagtggaat ggaaatgcag ccaaccggca atggcggccc gatgctcgga
     9421 aagtataaga gcgtgacatc ctgaaatcaa gttgacggga cacttatggc tggcacatga
     9481 cggcctttcg ggatagcctc ttaacatcat ggaacgggaa actattgact ctagaatcat
     9541 gaatcccgct gtggatatcg caaccggtta aggattgtgc ccgtcccaca gaggaggggt
     9601 ccacgatgtt gcgcgtatac gcctggttca tcaatcgggc gggaacgggt ttcggcgtgg
     9661 ccgt
//
LOCUS       DAOPCO010000150         9658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6729_length_9658_cov_6.289597, whole genome shotgun sequence.
ACCESSION   DAOPCO010000150 DAOPCO010000000
VERSION     DAOPCO010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9658
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6729_length_9658_cov_6.289597"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>950
                     /locus_tag="PLS90_10890"
     CDS             <1..>950
                     /locus_tag="PLS90_10890"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HPK02950.1"
                     /translation="RQVGELLVGSYEDPELLARLAAGADVLTYEFENVPLRTVYQLAE
                     HHAVHPRPEALRVAQDRIREKRFFALHQVPTPPFLEARDRDGLEDAVRRLGVPCVVKT
                     RQLGYDGKGQFLVRDPHDLPRAWEALAFACQPDGCGLIVEAFVPFEREVSLLAVRSRG
                     GEVRCWPLIENHHRGGILRWSIAPAPDLPAALREQAEQYGRRIMEALNYVGVLAIEFF
                     VSTSGPFTGLVANEMAPRVHNSGHWTIEGAATSQFTAHLCAILGLPLGDVTVAGAYAM
                     LNFIGTLPPEAARAQLNGLYWHLYGKTPRPGRKLGHATAPL"
     assembly_gap    951..960
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1159..2217)
                     /gene="rfbB"
                     /locus_tag="PLS90_10895"
     CDS             complement(1159..2217)
                     /gene="rfbB"
                     /locus_tag="PLS90_10895"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPK02951.1"
                     /translation="MSQPVADPPRHTPRCMLVTGGAGFIGSNFVRQLLRERPGLRLIN
                     YDALTYAGNLRSIADLDGHDGYLFVRGDICETERVAALLREQRVDTIVHFAAESHVDR
                     SILAGAEFVRTNVVGTQSLLDAAMQVGVERFVQVSTDEVYGSLGPEGRFTEETPLQPN
                     SPYSASKAGADLLCRAAFHTHGFPVVITRCSNNYGPYQFPEKLIPLMIMRALADEALP
                     VYGDGMNVRDWIHVEDHAAAVLAAAERGRPGEVYNIGADNEWPNIEIVRLILRSLGKP
                     ESLIRFVKDRPGHDRRYAIDSAKARRELGWAPRISFEEGIARTIAWYREHGDWLSSII
                     TGEYREYYEKNYAHKMQA"
     gene            complement(2214..3098)
                     /gene="rfbA"
                     /locus_tag="PLS90_10900"
     CDS             complement(2214..3098)
                     /gene="rfbA"
                     /locus_tag="PLS90_10900"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPK02952.1"
                     /translation="MMTKGIILAGGAGSRLYPLSLVASKQLQPVYDKPMIYYPLTTLM
                     LAGIRRICLISTPHDTPRFEALLGDGAQWGLSITYRVQPEPRGIAQAFLIAEDFIADD
                     PVTLILGDNIFHGRFEFVNIFGQFTRGGHIFAYHVHDPERYGVVEFDKDGRVLSIEEK
                     PRQPRSSFAVPGLYLYDERVVEICKGLKPSARGELEITDVNRAYLELKELRVSLLGRG
                     TAWLDTGTTTSLQEASNFISIIEKRQGYKIGCPEELALRKGFISFDDFIRILDGLPRC
                     EYADYLRGVAEEYQEFPT"
     gene            complement(3342..3539)
                     /locus_tag="PLS90_10905"
     CDS             complement(3342..3539)
                     /locus_tag="PLS90_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HPK02953.1"
                     /translation="MADLEPHDPTELEDEPRPSLIQEFIWFLRENKKWWLVPLILMFL
                     ALGALIFLGNSPVMPFIYTLL"
     gene            complement(3828..5243)
                     /locus_tag="PLS90_10910"
     CDS             complement(3828..5243)
                     /locus_tag="PLS90_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007154099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK02954.1"
                     /translation="MALSVNTNVASLNAQRNLSGNSQILGKALSRLSSGLRINQAADD
                     AAGLAISTGLTAQVRGLNQAVRNAGDGLSLLGTAESAITEQTNILQRIRELAVQAAND
                     TNSTTNRSSLNDEVMALVSELDRIAGTVEFNGLKLLDGTFTAKEIQVGAYAGSNQKIS
                     VDINGTRATAIGSPYEIAGTAVTTGGFTEGQVKITVNGQSFFAAATANDGVSSTDQGG
                     SALAYATAINAVSGSTGVRASASTSVTGAAQQVANHDTVDATNFLKINGVDVGAFNVT
                     ATDSTAVIDAINAKFSQTGVKASLGDDDKITLTAEDGRNIEVAAGGDAATASGLSSGT
                     TRGTVTLSSTQEFTVADSSGNLNADGTAAKNAAKNVNTVDISTKAGAELAIQIVDVAL
                     ENLNSSRAGIGAAVSRLNSVISNLSTVSENVAASNSRILDADFAAETANLTRGQILQQ
                     ASVAVLAQANQMPQLALSLLR"
     gene            6231..8036
                     /locus_tag="PLS90_10915"
     CDS             6231..8036
                     /locus_tag="PLS90_10915"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPK02955.1"
                     /translation="MSSPNAKLNQWVQQMAELCQPEAVYWCDGSAAEYDEMCRRMVAS
                     GTFVKLNEEKKPHSFLCRSDPSDVARVEDRTFICAQTKEEAGPTNNWMAPDECKTLLQ
                     GKFNGAMRGRTMYVIPFSMGPIGSPIAHIGIELTDSPYVVANMHIMTRVGARVLEVLG
                     PDGEFVPCLHSVGAPLAPGQKDVPWPCNTEKYITHFPDERLIMSYGSGYGGNALLGKK
                     CFALRIASVMARDEGWLAEHMLILAITSPEGRKYHIAAAFPSACGKTNLAMLIPTLPG
                     WQVRTIGDDIAWMKFGADGRLYAINPEAGFFGVAPGTSYDTNPMAMDSLQRNSIFTNV
                     ALTPDGDVWWEGMTDQPPAELIDWKYQPWTPDSGRLAAHPNARFTAPAAQCPVIDPAW
                     EDPAGVPIDAILFGGRRASLVPLIHQSFNWRHGTFLGSIMSSEKTAAAAGKVGEVRRD
                     PMAMLPFCGYNMGDYFQHWLNIGERSSAEKLPKIFYVNWFRKSPEGRFLWPGYGENSR
                     VLAWVINRVAGKAEARETPIGYVPTPEAIDTSGLDIPADSMKSLLAVDPEEWRAELPL
                     IKEHFEQFGERLPAALWEEYRALAQRLDGAAGAAG"
     gene            8380..8616
                     /locus_tag="PLS90_10920"
     CDS             8380..8616
                     /locus_tag="PLS90_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPK02956.1"
                     /translation="MADKDAKYPDNVPGKFYVDDTCIDCDACRAAAPDNFQRNEEGGY
                     SFVAKQPETEEEQQLCIEAMEGCPVEAIGDDGEE"
     regulatory      8748..8871
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8972..>9658
                     /locus_tag="PLS90_10925"
     CDS             8972..>9658
                     /locus_tag="PLS90_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02957.1"
                     /translation="MAACGAFRPSQHQSAFVSQRSAAARPKRSGFTLIELLIVVAIIA
                     ILAAIAVVNFKLAHDRALQSADAANLHTIATALQTYVVDHNVLPPADREAGPFPSHGP
                     EFTQVMNGPAAGGSHDGLPWLLVEQGYISDPKTLFCPKYLKLYSGGQTHRGGWPRYHN
                     FRYAYNAAAVSSGGAGAHGVMTGEVWIVRDLYIPAEAGWWAEAAPEYPGDYEYPWGED
                     NQFEFALYSDM"
BASE COUNT         1596 a         3080 c         3352 g         1620 t
ORIGIN      
        1 cgccaggtcg gcgagctgct cgtgggcagt tacgaggacc cggagctgct ggcacggctc
       61 gccgccggcg ccgacgtgct gacctacgaa tttgaaaatg ttcccctgcg caccgtctat
      121 cagcttgcgg agcatcacgc ggtgcatccg cgccccgagg cgctgcgcgt cgcccaggac
      181 cggatccgcg agaagcgttt cttcgcgctg caccaggtgc cgaccccgcc gttcctggaa
      241 gcgcgcgacc gggacgggct ggaggatgcg gtccgccgac tgggggtccc gtgcgtcgtc
      301 aagacgcggc aactgggcta cgacggcaag ggccagttcc tcgtgcgcga cccgcacgac
      361 ctgccccggg cgtgggaggc gctggcgttc gcctgccagc ccgatggctg cgggctgatc
      421 gtcgaggcgt tcgtgccgtt cgagcgcgag gtgtcgctgc tggccgtccg ctcgcgcggg
      481 ggcgaggtgc gctgctggcc gctgattgaa aatcaccatc gcggcgggat cctgcgctgg
      541 tcaatcgcgc cggcgcccga cctgcccgcc gcgctgcggg agcaggccga acaatacggc
      601 cggcgcatca tggaggcgct caattacgtc ggcgtcctgg cgatcgagtt tttcgtttcc
      661 accagcggtc cgttcaccgg cctggtggcc aacgagatgg ccccgcgcgt ccacaactcc
      721 ggccactgga cgatcgaagg ggccgccacc agccagttca ccgcccacct gtgcgcgatt
      781 ctggggttgc cgctgggcga tgtgacggtc gccggcgcct atgccatgct caacttcatt
      841 ggaacgctgc cgcccgaggc cgcgcgcgcg caactgaacg gcctgtactg gcatctgtat
      901 gggaaaacgc cgcggccggg gcgcaaactc ggtcatgcga cggcccccct nnnnnnnnnn
      961 ggcgctcggg attgaatgat cgcggccgaa tgattgcgcg aacgggtggg cgtaattacg
     1021 gcgggttttc gcgagccgag gacgagggac gagagccgag aaccgaattt tgggggagcc
     1081 ctctgggtgc gatcacgagg acgatggacc agagacgagg gacggttttg gcgtgagaaa
     1141 tgagggacga atttgtggct aggcctgcat cttgtgggcg taattttttt cgtaatactc
     1201 gcggtactcg ccggtgatga tgctgctgag ccagtcgccg tgttcgcgat accacgcgat
     1261 cgtgcgggcg atcccctctt caaatgaaat ccggggcgcc cagcccagtt cgcggcgggc
     1321 cttcgccgaa tcgatcgcat agcgccggtc gtggccgggg cgatccttga cgaagcggat
     1381 gagcgactcg ggcttgccca gcgagcgcag gatcagccgg acgatctcga tgttgggcca
     1441 ttcgttgtcg gcgccgatgt tgtagacctc gcccggccgg ccgcgctcgg ccgccgccag
     1501 cacggccgcg gcatggtcct cgacgtgaat ccagtcgcgc acgttcatcc cgtcgccgta
     1561 gaccggcagc gcttcatcgg ccagcgcgcg catgatcatc agcgggatca gcttctccgg
     1621 aaactggtag ggaccataat tgttcgagca gcgcgtgatg acgacgggga agccgtgcgt
     1681 atgaaacgcc gcgcggcaga gcaggtcggc gccggccttg gaagccgaat acgggctgtt
     1741 gggctggagc ggcgtctcct cggtgaagcg gccttcgggt ccgagcgaac catagacctc
     1801 gtcggtggag acctgcacga accgttcgac gccgacctgc atcgccgcat cgagcaggga
     1861 ctgcgtgccg acgacgttgg tgcggacgaa ttcggcgccg gcgaggatgc tgcgatcgac
     1921 atgcgactcg gcggcgaaat gcacgatcgt atcgacccgc tgctcgcgca gcaacgcggc
     1981 cacgcgctcg gtctcgcaga tgtcgccgcg cacgaaaagg tagccgtcgt ggccgtccag
     2041 atcggcgatg ctccgcaggt tgccggcata ggtcagcgcg tcgtaattaa tcaggcgcag
     2101 gccggggcgt tcgcgcagca gctggcgcac gaaattggag ccgatgaacc cggcgccgcc
     2161 ggtcaccagc atgcagcggg gggtgtgacg cggcgggtcg gcaaccggct ggctcatgtc
     2221 gggaactcct gatactcctc ggcgacgccg cgcagatagt cggcgtattc gcagcgcggc
     2281 aggccatcga gtatcctgat gaaatcgtcg aacgaaataa agcccttgcg cagggcgagt
     2341 tcctcggggc agccgatctt gtagccctgg cgcttctcga tgatcgagat gaagttgctc
     2401 gcctcctgca ggctggtcgt cgtgccggta tcgagccagg cggtgccgcg gcccagcagc
     2461 gagacgcgca gttccttcag ttcgagatag gcgcggttga cgtcggtgat ttccagctcg
     2521 ccgcgggccg agggtttgag gcccttgcag atctccacga cgcgctcatc gtagaggtag
     2581 agccccggca cggcgaaact cgacctgggc tggcgcggtt tctcctcgat gctgagcacg
     2641 cgcccgtcct tgtcgaactc gacgacgccg tagcgttccg gatcgtgcac atgataggcg
     2701 aagatgtggc ccccccgggt gaactggcca aagatgttca cgaattcgaa gcggccgtgg
     2761 aagatgttgt cgccgagaat gagcgtgacg gggtcgtcgg cgatgaagtc ctcggcgatg
     2821 aggaacgcct gcgcgatccc ccggggttcg ggctggacgc gataggtgat ggagaggccc
     2881 cactgcgcgc cgtcgccgag cagcgcctcg aagcgcggcg tgtcgtgggg cgtggaaatc
     2941 aggcagatcc ggcggatgcc ggccagcatc agcgtcgtca gcgggtaata gatcatcggc
     3001 ttgtcgtaga ccggctgaag ctgcttgctg gcgacaaggc tcagcgggta gagccggctg
     3061 ccggcgccgc cggccaggat gatccccttg gtcatcatga atcccgaatt ccttggcttg
     3121 aatagggccg cggcgcgcgc ggcacgcacg cggggccctg cccgcaactg ccgcggggcg
     3181 ccggcgcgcg cccggtcgcg ccgtcagcac agcatttgag gcgtattccc cggacgggtc
     3241 ctcattcttg aaggcagtcg ccgcaatcgt caagcgggat atggccggat tgcgatggcc
     3301 gaggacgagg gacgaaccgg cgccaggggg gaggcggctc gctacagcag agtatagatg
     3361 aacggcatca ccgggctgtt gccgaggaag atcagcgcgc ccagcgccag gaacatcagg
     3421 atcagcggca ccagccacca cttcttgttt tcgcgcagga accagatgaa ctcctgaatc
     3481 aacgacggcc gcggttcgtc ctccagttcg gtcggatcat gcggttcgag gtcggccatc
     3541 ggaagactcc ggagcggagg ggtcggcccg gggcggcgcc ggcgggccgc cgcggacggg
     3601 tgcgtggctt cattttacgg aatcgcgcga gcaatccgca accatcggcc gcgcattttg
     3661 ggtgccgttc gccgcgtcac cgcgcgcgct cccctcggcc ggataaaaag gcgccccagc
     3721 gtgggggcgc cggggcggat ccgttgaacg ggacggtatg tggttcttcg gcgttccggg
     3781 cgtttcaatg cgcccggagg tacaacacct tcgaccgtcg ccgcgcgtca gcgcaggagc
     3841 gaaagcgcaa gctgcggcat ctggttggcc tgggccagga cggcgaccga ggcctgctgc
     3901 aggatctggc cgcgcgtcag gttggccgtc tcggccgcga aatcggcatc caggatgcgc
     3961 gaattggagg cggcgacatt ttcgctgacg gtgctcaggt tgctgatcac cgagttgagt
     4021 cggctgacgg cggcgccgat gcccgcgcgc gaggagttga ggttctccag cgcgacgtcg
     4081 acgatctgaa tcgccagctc cgccccggcc ttggtcgaga tgtcgaccgt gttgacgttc
     4141 ttggcggcat tcttggccgc cgtcccgtcc gcgttcaggt tgccggagct gtcggccacg
     4201 gtgaattcct gggtgctgga cagcgtgacg gtgccgcggg tcgtgcccga gctcaggccc
     4261 gaggcggtgg ccgcgtcgcc gccggccgcc acttcgatgt tgcggccgtc ctcggccgtc
     4321 agcgtgatct tgtcgtcgtc gccgagcgag gccttcacgc cggtctggct gaacttggcg
     4381 ttgatcgcgt cgatcaccgc cgtcgaatcg gtcgccgtca cattgaacgc gccgacgtcg
     4441 acgccgttga tcttcaggaa gttggtcgcg tcgaccgtat cgtggttggc cacctgctgg
     4501 gccgcgccgg tgacgctggt cgaggccgag gccctgacgc cggtcgagcc gctgaccgcg
     4561 ttgatcgccg tggcgtaggc cagcgccgag ccgccctggt cggttgagga gaccccgtcg
     4621 ttggccgtcg ccgccgcgaa gaagctctgg ccgttgaccg tgatcttgac ctggccctcg
     4681 gtgaagcccc ccgtggtgac cgccgtgccg gcgatctcgt acgggctgcc gatggcggtg
     4741 gcgcgggtgc cgttgatatc aaccgaaatc ttctggttcg agccggcgta ggcgccgacc
     4801 tggatttcct tggcggtgaa cgtgccgtcc agcagcttga ggccgttaaa ttcgacggtg
     4861 ccggcgatgc ggtcgagctc ggagaccagc gccatgacct cgtcattgag cgaggagcgg
     4921 ttggtggtgc tgttggtgtc gttggccgcc tggaccgcca gttcgcggat gcgctgcagg
     4981 atgttggtct gctcggtgat cgcgctctcg gcggtgccca gcagcgagag gccgtcgccg
     5041 gcgttgcgga cggcctggtt gaggccgcgg acctgggcgg tcaggccggt cgagatggcc
     5101 agaccggcgg cgtcgtcggc cgcctggttg atccgcaggc cggaggacag gcgcgacagc
     5161 gccttgccga ggatttgcga attgcccgaa aggttgcgct gcgcattgag actggcaacg
     5221 ttggtattga cgctcagagc catgagaact ttcctccctg aaagtcccgc gtgggagttg
     5281 gcgaaggtca gccgggcatc cttgcccgtg acgcgatccg ccaagtggag atgatgggtc
     5341 cttccgaaga gcaaccggtc ggacgaaggg ccgcgttcct gatggccatc ctgacccccg
     5401 cgggctgccc ttgccgcggg cgcctcacaa ccagcttcca ggccgccctg gccgcgcctt
     5461 ccccgcgcga ggaccggttt ccgccgaggc gaagcgggat ccggccgaac cggcctgaag
     5521 ccgccgccga gcgggcggcg acgggggccg ttcgaatcaa ctcaaatcat gtgcggggga
     5581 agaaaaggag accgtttcca tcctggaagc ggcggggcgt tggctcggtc ggccttgccc
     5641 cacacaggaa ttatcgtccg tccgccggcc gacttaaggg ggaagtgtta ttttttttga
     5701 tacagtcggg ccggcgcggt gttgcctggc ggagacaggg gggaagggat ggaagggaca
     5761 tcagggacgt aagggacgta gggacagaaa ggatctgcgt gaggacggcc gcaatgagtc
     5821 gtcctcgtcc ttctcctcct gatcgccctc ggggatatct cgtaaattcg gctcccggct
     5881 cccgttcctg tccctggctc tcgaaatcgt cccgattctc ccacccgcca cgttcccgat
     5941 ttcagctgga aaaccgcacc cggtgatcgt ccggcttgta atccccgccg cgagacgcta
     6001 aacaggtagg gcgaaccggg tcgcccggcg cggcgcccgc gaggcgccgg catggggaac
     6061 ccgaccctcg gatgcgcgtt gacccgctcg ccgggcgccg cccggcgagt cgccccgatg
     6121 cagacgccga ggccgtccga ccggcgcatc cgatcccagg tcgtcgcccg cgcgacggat
     6181 cgccccgttc aacctgcccg cctcaacatc ccaggaaagg gtcccgacgt atgagcagcc
     6241 cgaacgccaa attgaatcag tgggtccagc agatggccga gttgtgccaa cccgaggccg
     6301 tctactggtg cgacggttcc gccgccgagt atgacgagat gtgccggcgg atggtggcct
     6361 ccggcacgtt cgtgaaactg aatgaagaga agaagcccca tagcttcctg tgccgctccg
     6421 atccgtcgga cgtcgcgcgc gtggaggacc gcacgttcat ctgcgcgcaa acgaaggagg
     6481 aggccggtcc gacgaacaac tggatggccc cggacgagtg caagacgctt ctgcagggga
     6541 aattcaacgg cgcgatgcgc ggccgcacga tgtatgtcat cccgttcagc atggggccga
     6601 tcggctcgcc gatcgcccat atcgggatcg agctgaccga ttcgccctac gtcgtggcga
     6661 acatgcacat catgacgcga gtcggcgccc gggtgctcga agtgctcggc cccgacgggg
     6721 agttcgtccc ctgcctgcac tcggtcggcg cgccgctggc ccccggccag aaggacgtgc
     6781 cctggccgtg caacaccgaa aagtatatca cgcattttcc cgacgagcgg ctgatcatgt
     6841 cgtacggctc gggttacggc ggcaacgcgc tgctgggcaa gaagtgcttc gcgctgcgga
     6901 tcgcctcggt gatggcgcgc gacgagggct ggctggccga gcacatgctg atcctggcga
     6961 tcacctcgcc cgaggggcgc aaataccaca tcgcggcggc gttccccagc gcctgcggca
     7021 agaccaacct ggcgatgctg atcccgacgc tgccgggctg gcaggtgcgg acgatcggcg
     7081 acgacatcgc ctggatgaag ttcggcgccg atggccggct ctacgcgatt aaccctgaag
     7141 ccggcttctt cggcgtggcc cccggcacca gctatgacac caacccgatg gcgatggaca
     7201 gcctgcagcg caattcgatt ttcaccaacg tggcgctgac gcccgacggc gacgtctggt
     7261 gggaagggat gaccgaccaa ccgccggccg agctgatcga ctggaagtat cagccgtgga
     7321 cgcccgacag cgggcggctg gcggcgcatc ccaacgcgcg gttcacggcc ccggcggcgc
     7381 agtgccccgt catcgatccg gcctgggagg acccggcggg cgtgccgatc gatgcgattc
     7441 tgttcggcgg ccgccgcgct tcgctcgttc cgctgatcca ccagagcttt aactggcgcc
     7501 atggcacgtt cctgggctcg atcatgagtt cggagaaaac ggcggccgcg gcggggaagg
     7561 tcggcgaggt ccgccgcgat ccgatggcca tgctcccgtt ctgcggctac aacatggggg
     7621 attatttcca gcactggctc aacatcggcg agcgttccag cgccgagaag ctgcccaaaa
     7681 tcttttacgt gaactggttc cgcaagagcc ccgagggccg gttcctctgg ccgggatacg
     7741 gcgaaaacag ccgggtgctg gcctgggtga tcaaccgggt ggcgggcaag gccgaggccc
     7801 gcgagacgcc gatcggctat gtgccgacgc ccgaggcaat cgatacgagc gggctggaca
     7861 tcccggccga ctcgatgaag agcctgctgg cggtcgatcc ggaggaatgg cgggcggaac
     7921 tgccgctgat caaggaacac ttcgagcagt tcggcgagcg cctgccggcg gcgttgtggg
     7981 aggagtaccg tgcgctggcg cagcggctcg acggggcggc cggcgccgcc ggctgacggc
     8041 ttggaagcac atggagatca cggcgcgcgg ggttgatctt tgtggttgat ttgcgccgcg
     8101 cgatccgcga aaaagtggtg caaatgcgct ggcggcgggg ggccgccgcc acgcggtcgg
     8161 cgggggggat ccgggctgcc cgacgcgggc gacaaccacg gaagcaaccg tttctcaggg
     8221 ttttgcggcc tttttgcgca aaccggccgc ccgaccggcc acagcaccgg ttcagcggac
     8281 cggacccaat cggtcgggta tggaccttgt tccaaccgac ggggcagatc cattcagaac
     8341 ggcccgagca tcggaccttt caggaggcag tagagttcca tggcagacaa agacgcaaaa
     8401 tacccggata acgtgcctgg caagttctac gtcgacgaca cctgcattga ctgcgatgcc
     8461 tgccgggccg ccgcgcccga caacttccag cgcaatgagg aggggggcta ctccttcgtc
     8521 gccaagcagc cggagacgga agaggaacag cagctctgca tcgaggcgat ggagggctgc
     8581 cccgtcgagg ccatcggcga cgacggcgaa gagtgaggca tcgcctccac cggcctgcgc
     8641 gggccggcgg aggcgatcct gttttcagga tctggttgac cgtattcccc tctttcccta
     8701 gcatattgac ggcatgaccg gcgatgccgg ggcgtcggcc ccggctcgga agatggtgcg
     8761 aatccatcgc ggacccgccg ctgtaaccgg tgacgaacgc cgcacaacgc cactggcccc
     8821 ccctggggct gggaaggcgc ggccagtagg atgacccgga agccagaaga cccgccggcc
     8881 cgccacgcga gaacccgccg cacggggcga tgcccgtccc gcgccggccg ttcccgccgt
     8941 tgacgactca tcttcttcga ggagtgagaa gatggcggct tgcggcgcct tccgaccttc
     9001 ccaacaccag tcagcgttcg tctcgcagcg ctctgcggcg gctcgtccga aacgatcggg
     9061 cttcacgctg atcgagctgc tgatcgttgt ggcgatcatc gcgatcctgg cggcgatcgc
     9121 cgtcgtcaac ttcaagctgg cgcacgaccg cgcgctgcaa tcggccgatg cggccaatct
     9181 gcacacgatc gccacggcgc tgcagactta cgtcgtcgat cacaacgtgc tcccccccgc
     9241 cgaccgcgag gcgggaccgt tcccgtcaca cggaccggaa ttcacgcagg taatgaacgg
     9301 accggccgcg gggggctcgc acgacggcct gccctggctg ctggtcgagc agggctacat
     9361 cagcgatccc aagacgctgt tctgtcccaa gtatttgaaa ctctactccg ggggccagac
     9421 gcatcggggg ggctggccgc gctatcacaa tttccgctac gcctacaatg ccgcggccgt
     9481 cagctcgggc ggggcggggg ctcacggcgt gatgaccggc gaagtgtgga tcgtccgcga
     9541 cctctatatc cccgccgagg ccggctggtg ggccgaggcc gcccccgaat accccggcga
     9601 ttacgaatac ccctggggcg aggacaatca gttcgagttc gcgctctaca gcgacatg
//
LOCUS       DAOPCO010000151         9617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6761_length_9617_cov_4.699540, whole genome shotgun sequence.
ACCESSION   DAOPCO010000151 DAOPCO010000000
VERSION     DAOPCO010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6761_length_9617_cov_4.699540"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1989
                     /locus_tag="PLS90_10930"
     CDS             43..1989
                     /locus_tag="PLS90_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02958.1"
                     /translation="MIHGRASISWVLLLAAARFALAQEGGLAGDATELLLTDSLSPFV
                     HRITLYDADGVAIAPDDIHPKPYSPARTCGKCHPYGAIGDGWHFNAARPEAPAGRPGE
                     PWFLVDLPTGTQAPLSYRDWPGAWDPAVFGLTEWEMIERFGRHQPGGGIGERPADGES
                     EPDPDAPWATAGRLEIDCLACHSAAAGFDQTQRAEQIAQRNLAWAPTATAGFGIVRGS
                     TKNLPADFNPDFDTPPADATQLEYDATQFDANDRVFFDVTRGIPAERCYFCHTTRVDR
                     PVDDLAGAPPRRWLSEGDVHLAAGLSCVSCHRNGLDHQIVRGYADERPGDPFAQAFTC
                     AGCHYGNAEADDPALARGGRLGSPRSDHPGIPPLHFEELSCTACHAGPLPGETARRVQ
                     TAMAHGLGLADRHRPYDDPPFIQEPVFLEGDDDVYRPYRLMWPSFWGRVGTAGEVTPL
                     APEEVMGTARAALGARRGMKPGAWQPLETGQIAEALAALAEPDGGGEPVYISGGVTYR
                     LSDDALTTETGWAAAAPYTWPLAHDVRPAAQALGAGGGGVSGCLDCHAPDSPFYFGKV
                     AAEGLVAPRRAALLPMHMLMGEDAKLLGAWGLSFAWRTLLKVIGLISAGVIAVVLALY
                     GAAGGRWLLRSCGRGGGADPGEGI"
     gene            1989..2519
                     /locus_tag="PLS90_10935"
     CDS             1989..2519
                     /locus_tag="PLS90_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02959.1"
                     /translation="MFRLWILATIVAVTLATTLHSVRTAGRGPGAAAPLPSRLRRLGR
                     VCYLLVLAGVGLAGLTGVVGAGLRGVPMEGYGLLLHVAAGGVFAPALAALAVLWADRN
                     RFGSPQPFSLGQKICFWMMLACGLVMILTGIGPMFRLFGTEAQRAVIAIHRHAALALL
                     ATAIFHIYLSVRYRGV"
     gene            2547..2966
                     /locus_tag="PLS90_10940"
     CDS             2547..2966
                     /locus_tag="PLS90_10940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HPK02960.1"
                     /translation="MATIFTRIIEGELPGRFVWKDEQCVGFLSINPLQPGHVLVVPRV
                     EVDHWLDLAPELTAHLTQVAQIIGQAQQAVFNPVKVGLMIAGLEVPHVHLHVVPIRGV
                     HDLDFANAAKNPDPAELDRAAERLRAQLKKMGRAEAA"
     gene            3041..3721
                     /locus_tag="PLS90_10945"
     CDS             3041..3721
                     /locus_tag="PLS90_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPK02961.1"
                     /translation="MNRLLHLGLAGALALACWLGAAAVAADEELEELKLELPKPMFKG
                     TPENIESANLEPPRQGPPPAVLVPKGTINLSRDKEVSASDEEPIVGELAFITDGDKEG
                     NDGSFVELGPGQQWVQIDLGAPARIHAIALWHFHSQARVYRDVVIQTADDPDFIENVV
                     TIFNNDHDNSAGIGVGKDKEYVETFEGRLIPVEGVEGRYVRLYSNGNTSNDLNHYVEV
                     EVYGKPVE"
     gene            3955..4536
                     /locus_tag="PLS90_10950"
     CDS             3955..4536
                     /locus_tag="PLS90_10950"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPK02962.1"
                     /translation="MEPPRPKADEILIRPEPTPNPDSVRFIVSERLLEGGTADLKRSA
                     SALERSPLARKIFDACEDVQGVFLGPDFVTITARETADWPTIADAVDVIVRQHLAAGE
                     PVLLGDADAAHGAAADTEVARGIARIIDAEIRPAVAMDGGDVIFGGYEDGIVSLYLRG
                     ACHGCPSSMMTLKMGIERRLKEEFPEIVSVEAI"
     gene            complement(4580..4900)
                     /locus_tag="PLS90_10955"
     CDS             complement(4580..4900)
                     /locus_tag="PLS90_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02963.1"
                     /translation="MARRKAARANPSGWGRTLGLIVACLALGAGAALFSRGGMEAARQ
                     WLREQTLARADGPPPVEIDPEMSRAALERENQRLREQLEAKDRELAELTIQLKLMEEG
                     SRSE"
     gene            complement(4919..6091)
                     /locus_tag="PLS90_10960"
     CDS             complement(4919..6091)
                     /locus_tag="PLS90_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPK02964.1"
                     /translation="MQQRCRKWLAGPGSLVLCAALLLAAGCARAPRTITKVTPAPPER
                     VVLDYRNGWLHPADGRERIGEIARRYDREPALVALLNRAAEDTVPAAATSIYIPPTNN
                     RESLRGVLARIQNHPELVPDRPWAPPQETVKVAQAQTKRVIPSKSATADLKQRLHEAP
                     LLRNPPQPEPEVKVAAASAAALPVLEDAFPPAPKKPAAHKTAVAQAAPILSKASVEGG
                     NPVPKPAKKRAGVLTGFKTGPVAIEASQPAKSAAKPAVAPQPEVGGDAPSAAGFIWPA
                     KGRIVSRFQEGWRKACHGIEIATAENAAVRAARDGKVLLAQQFMSYGNLIVIDHGDGY
                     ASAYGYNDKVLVKEGDQVKRGQQIASAGRRYRSGEPMLFFQIRHNMKPVDPLTYLN"
     gene            complement(6406..8025)
                     /locus_tag="PLS90_10965"
     CDS             complement(6406..8025)
                     /locus_tag="PLS90_10965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK02965.1"
                     /translation="MNRFAWRVPPGPLLPLAVILCLGGWLRLTHLNAIPPGLWLDEAL
                     NGQDAVAVWQPGGEGFRMVYPEEYPREPLYITLLACAVRVLGPVPVTLRLVSALIGIA
                     ALVLVFGMARALAGPRVGLLAAAVLATLFWHVLFSRLVFRTNLLAPWMAAFVWTVVAW
                     RRRPALWRAALAGVLIGGGFYTYLAWYFMALLWIPLVLWIASRDRAGAAPRPPTSTRL
                     CLGLAALVALPLLVHYVRHPEDLMARAGQVSVTNLPPTAAAAEVFENAWEALGMFHLT
                     GDHVAKQNLPWRPALDPVQGLFFLIGLALCARSLRSAGGLPRPHGAILLGWIAAGLLP
                     TIFTITDSPNFLRTLVLTPAVATVTALGLAWAAERAAARTGRIAALSLLALALGYSTF
                     QSHRTLHEWSRREDVYLAFQGDLADIARFAQTVPPEAPLYVPDFFGLFRSFQFQTIGL
                     DNLYYYKDWSFLGPWPTDPRQAGLPPPPYRIVIATAANRAYEPLRALGGRDARFFARP
                     GGAVWGAAVAFLESDLPDTATLRLLPPLPSF"
     assembly_gap    8202..8301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8434..>9617
                     /locus_tag="PLS90_10970"
     CDS             8434..>9617
                     /locus_tag="PLS90_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK02966.1"
                     /translation="MVEGAGKRAVILGAGVAGLATALRLAEGGGWRPRLLEREDRPGG
                     LARSLHFRGVSTDLGPHRIHTEIPEVQALIESVAARSLVRLRRSSHIFLRKKWLTYPP
                     RPLELLGALGPIRMARFGTGFALEKLRPRPAQETYESLMRAAFGGPLWEFLLRPYSAK
                     TWKIDPGELHADTARVRVSAGSLGKLVAGLLRREQQGAETALREFRYVKGGAETLVRH
                     LWAAAHAAGAELELNTAVQELELDERGRVRAALALRRPAHGTAEGADQRFEGELFIST
                     VPLPILLGRLLPQRAELMAAREAAARLEYLDMAFVCLIVRRKVITGDNWLYFPEPHLI
                     FNRGYEAKSFDPAMAPEDKSVICLEITMRRGDALARESDALILQTATEHIVSTGLFRK
                     EE"
BASE COUNT         1532 a         2923 c         3661 g         1401 t
ORIGIN      
        1 gagagacgac acttaatgcg ggggggcgcg gggagccggg ggatgattca tggcagggca
       61 agtatttcct gggttttgtt gctcgccgcg gcgcgtttcg cccttgcgca ggagggcggt
      121 ctcgccggcg acgcgacgga gttgctgctg accgacagcc tgtcgccgtt cgtgcaccgg
      181 atcacgctgt atgatgccga cggggttgcg attgcgcccg acgacatcca tcccaagccg
      241 tattcgcccg cgcgcacgtg cggcaaatgc catccctacg gcgcgatcgg cgacggctgg
      301 catttcaacg cagcgcggcc cgaagccccc gccggccggc cgggcgagcc ctggttcctg
      361 gtcgacctgc cgaccgggac gcaggcgccg ctttcgtatc gcgactggcc gggagcgtgg
      421 gacccggcgg tgttcggtct gaccgaatgg gaaatgatcg agcgtttcgg ccgccaccag
      481 ccggggggtg ggatcggcga gcgccccgcc gacggcgaga gcgagccgga tccggacgcg
      541 ccgtgggcaa cggcgggacg cctggagatc gactgcctgg cctgccacag cgccgccgcc
      601 gggttcgacc agacgcagcg cgccgaacag atcgcgcagc ggaacctggc ctgggcgccg
      661 accgcgacag ccgggttcgg catcgtgcgg ggaagcacca aaaacctgcc cgcggatttc
      721 aatcccgatt tcgacacgcc gccggccgac gcgacgcagc ttgaatacga cgccacccag
      781 ttcgacgcga acgaccgcgt gttcttcgat gtcacgcgag ggattccggc cgaacgctgc
      841 tacttctgcc acacgacgcg ggtcgaccgg ccggtcgacg atctggccgg cgcccctccc
      901 cggcgctggc tgagcgaggg agacgtccac ctggcggcgg ggctgagctg cgtgagctgc
      961 caccgcaacg ggctggacca ccaaatcgtg cggggctatg cggatgagcg gccgggcgat
     1021 ccgttcgcgc aggcgttcac ctgcgccggc tgccactacg gcaacgccga ggcggacgac
     1081 ccggcgctgg cgcgcggggg ccggctcgga tcgccgcggt cggatcatcc cggcatcccg
     1141 ccgctgcatt ttgaggaact gagctgcacg gcgtgccacg ccgggccgct cccgggcgag
     1201 acggcgcggc gtgtgcagac ggcgatggcg cacggactgg ggctggccga ccgccaccgc
     1261 ccctacgacg acccgccgtt cattcaggag ccggtgttcc tggaaggcga cgatgatgtt
     1321 taccggccgt accggctgat gtggccgtcg ttctggggcc gggtcgggac ggccggggag
     1381 gtgacgccgc tcgccccgga agaagtgatg ggaacggccc gcgcggccct cggcgcgcgc
     1441 cgggggatga aaccgggagc gtggcagccg ctggagaccg gccagatcgc cgaggcgctg
     1501 gccgccctgg ccgaaccgga cggcggcggc gagccggtct acatcagcgg cggcgtgacc
     1561 taccgtctca gcgacgatgc cttgacgacc gaaaccggct gggcggcggc ggcgccgtat
     1621 acctggccgc tggcgcacga cgtgcggccg gccgcgcagg cgctgggcgc cggcggcggg
     1681 ggcgtgtcgg gctgtctgga ttgccacgcc cccgattcgc ccttctactt cggcaaggtg
     1741 gccgcggagg ggctcgtcgc gccgcggcgg gccgccctgc tgccgatgca catgctgatg
     1801 ggcgaggatg cgaagctgct gggggcgtgg gggctctcgt ttgcctggcg gacgctgttg
     1861 aaggtcatcg gactgatttc ggcgggagtg attgccgtgg tcctggcgct gtatggcgcg
     1921 gcgggggggc gctggctgct gcgatcgtgc gggcgcggcg gtggcgccga ccccggggag
     1981 ggcatctgat gtttcggctg tggatcctgg caacgatcgt ggcggtgacg ctggcgacca
     2041 cgctgcactc agtgcgtacg gccgggcgcg gtcccggggc ggccgctccc ctcccctcca
     2101 gactgcgcag gctggggcgc gtctgttacc tgcttgtgct ggccggcgtg ggtctggccg
     2161 ggctgacggg cgtggtgggg gcggggctgc gcggcgtccc gatggaaggc tatggcctgc
     2221 tgttgcacgt cgcggcgggc ggggtgttcg cccctgcgct ggcggcgctg gccgtgctct
     2281 gggccgaccg caaccggttc ggttcgcccc agccgttcag cctgggccag aagatctgtt
     2341 tctggatgat gctggcgtgc gggctggtga tgatcctgac ggggattggg ccgatgttcc
     2401 ggctgttcgg gaccgaggcg cagcgcgccg tgatcgcgat ccatcgccac gccgcgctgg
     2461 cgctgctggc cacggcgatc ttccacatct acctgtcggt acggtatcgt ggcgtgtaga
     2521 tcgatgcgtg cggaaaggga tggatgatgg caacgatctt cacgcggatc atcgagggcg
     2581 agcttccggg gcggttcgtc tggaaggacg agcagtgcgt cggttttctg tcgatcaatc
     2641 cgctgcagcc gggccacgtg ctggtggtgc cgcgcgtcga agtggaccac tggctggatc
     2701 tggcgcccga gctgacggcg catctgacgc aggtggcgca gatcatcggc caggcccagc
     2761 aggcggtgtt caatccggtc aaggtgggcc tgatgatcgc cggtctggaa gtgccgcatg
     2821 ttcacctgca cgtggtgccg attcgcggag tacatgatct ggatttcgcc aatgcagcga
     2881 agaaccccga tccggccgaa ctggaccggg ccgccgagcg gctgcgcgca caactgaaga
     2941 agatggggcg cgccgaggcg gcgtaggatg gacggcgagg cggtcgcggg agaccgatag
     3001 cgataccgat tgcgatgcct tttggggaag gatgacgaag atgaatcgac tgctacactt
     3061 gggattggcc ggcgcgctgg cgctggcctg ctggctcggc gccgcggcgg tggcggccga
     3121 cgaagagctg gaggaactca agctggagct gcccaagccg atgttcaagg gaacgcccga
     3181 gaacatcgaa tcggccaatc tggagccgcc gcgccagggc ccgccccccg cggtgctggt
     3241 gcccaagggg acgatcaacc tgtcgcgcga caaggaggtc agcgcgagcg acgaggaacc
     3301 gatcgtcggc gaactggcgt tcatcaccga cggcgacaag gaagggaacg acggcagctt
     3361 cgtggagctg gggccgggcc agcagtgggt gcagatcgac ctgggcgcgc cggcgaggat
     3421 ccacgccatc gcgctgtggc atttccattc gcaggcccgc gtctatcgcg acgtggtgat
     3481 ccagacggcc gacgaccccg atttcatcga gaacgtcgtg acgatcttca acaacgatca
     3541 tgacaactcg gccggcatcg gcgtcggcaa ggacaaggaa tacgtcgaga cgttcgaggg
     3601 gcggctgatc cccgtcgagg gcgtcgaggg acgctatgtg cggctctatt ccaacgggaa
     3661 cacctccaac gacctgaacc actacgtgga ggtggaggtt tacggtaaac cggtggagta
     3721 acgccggcgc gaggcggccc gcccgccccg gaccccggga cgggtgccat tgaccggccg
     3781 ggcggcgggg tgattcaatg gaggggcaat aggcccgtcc cgtcagggac ggcagacgtc
     3841 agcccgccac tttcagcggc gggaaatggg gtcgacagag cggaagagcg ggccttcggg
     3901 agcgatcacg aggacgaggg acgagagacg attttagaga aaggggagat tgacatggaa
     3961 cccccacgac cgaaggcgga tgagattctg atccggccgg agccgacgcc caatccggac
     4021 agcgtccggt ttatcgtcag cgagcggctg ttggaagggg ggacggccga tttgaagcgt
     4081 tcggccagcg cgttggagcg ctcgcccctg gcgcggaaga ttttcgacgc ctgcgaggac
     4141 gtgcaggggg tctttctggg accggatttc gtgacgatca cggcgcggga gacggccgac
     4201 tggccgacga tcgccgacgc ggtcgacgtg atcgtgcgcc aacacctggc ggccggcgaa
     4261 ccggtgctgc tgggcgacgc ggacgcggcg catggcgccg cggcggacac ggaggtcgcg
     4321 cgcgggatcg cgcggatcat cgacgcggag attcgtccgg cggtggcgat ggatggcggc
     4381 gacgtcatct tcggcggcta tgaagacggc atcgtcagcc tctacctgcg cggggcgtgc
     4441 cacggttgcc ccagttcgat gatgacgctc aaaatgggga tcgagcggcg gctgaaggaa
     4501 gagtttcccg agatcgtgtc ggttgaggcg atctgaaaaa gggcgccggc gggcccgacc
     4561 cgctccttcc aggccggtct tattctgacc gggagccttc ttccatcagc ttgagctgga
     4621 tggtcagttc ggccagttcg cggtccttgg cctcgagctg ttcgcgcagg cgctgattct
     4681 cgcgctccag ggcggcgcgg ctcatttcgg gatcgatttc cacgggaggc gggccgtcgg
     4741 cgcgcgcgag ggtttgctcg cgcagccact ggcgcgcggc ctccattccg ccgcggctga
     4801 acagcgcggc gcccgccccg agcgccaggc aggcgacgat caggcctagc gtgcgtcccc
     4861 agccgctggg gttggcgcgg gcggctttcc tgcgggccat ggcgcgacgg ttccccggct
     4921 agttcagata cgtgagcgga tcgacgggct tcatgttgtg gcggatctgg aagaagagca
     4981 tcggctcgcc ggagcgataa cggcgaccgg cgctggcaat ctgttgaccg cgcttgacct
     5041 ggtcgccctc cttgaccagc accttgtcgt tatagccata ggcgctggcg tagccgtcgc
     5101 cgtggtcgat cacgatcaag ttgccgtaac tcatgaattg ctgggccagc agaaccttgc
     5161 catcgcgcgc ggcgcggacg gcggcgttct cggcggtggc gatctcaatc ccgtggcagg
     5221 ccttgcgcca gccctcctga aaccgcgaga cgatgcgccc cttggccggc cagatgaagc
     5281 cggcggcgga gggagcgtcg ccgccgacct cgggctgggg cgcgacggcc ggttttgctg
     5341 cggatttcgc gggctgcgac gcctcgatcg cgactggtcc cgttttgaaa cctgtcaaga
     5401 cgccggcgcg cttcttcgcc ggtttgggaa cggggttccc cccttcgacc gatgccttgg
     5461 agaggatcgg cgcggcctgg gcgacggcgg tcttgtgcgc cgcgggcttc ttgggtgccg
     5521 gcgggaaggc gtcctccagc accgggagcg cggcggccga ggccgcggcg accttgacct
     5581 ccggctcggg ctgcggcggg ttgcgcagca acggggcttc gtggagccgc tgcttgagat
     5641 ccgcggtggc ggacttggag gggatgacgc gcttggtctg cgcctgggcg accttgactg
     5701 tttcctgagg gggcgcccac ggccggtcgg ggacgagttc ggggtggttc tgtatccggg
     5761 caagcacgcc gcgcaggctc tcccggttgt tcgtgggggg gatgtagatg ctggtggcgg
     5821 cggcggggac ggtgtcctcg gcggcgcggt tgaggagcgc gacgagcgcc ggttcgcggt
     5881 cgtagcggcg ggcaatctcg ccgatcctct cgcggccgtc ggccggatgc agccagccgt
     5941 tgcggtaatc caggacgacg cgctcggggg gcgcgggcgt gactttggtg atcgtgcgag
     6001 gggcgcgcgc gcagccggcg gccagcagga gcgcggcgca cagcacgagc gagcccgggc
     6061 ccgcgagcca tttgcgacaa cgttgttgca tgcgtggaac ggtgggaggg ttcatcgcgg
     6121 cggctggccc aacggccggg tgcttcaacg ggttgcgcga aacagcgatg gcgatttcgc
     6181 cgactcgacg attcgaccca agtgaatacc ccaagccggc gcgaaatgca aatctttccg
     6241 tggcgccaag agcgtgcggg ggggagaaga cgggatcacg aaaggcacga aagggcacgg
     6301 aagggtcggc aggcaaaacc ttggcgagga caaacggcgt ccttccgcgt gagccgcccg
     6361 tcccttgtgt ccctgacgtc ccccatgtcc ctactctgct gaacctcaga acgaggggag
     6421 cggggggagg agccggaggg tcgcggtgtc gggcagatcg gattcgagga aggcgacggc
     6481 ggcgccccag acggcgccgc cggggcgcgc gaagaaccgc gcgtcgcggc cgccgagcgc
     6541 gcgcagcggc tcataggccc gattggccgc cgtggcgatg acgatccgat acggcggggg
     6601 cggcagcccg gcctggcgcg gatcggtcgg ccagggaccc aggaacgacc agtccttgta
     6661 gtaatagagg ttgtccagac cgatcgtctg gaactggaac gagcggaaca ggccgaagaa
     6721 atcgggaacg tagagcggcg cctcgggcgg cacggtctgg gcgaaccgcg caatgtcggc
     6781 caggtcgccc tggaaggcga gatagacgtc ctcgcggcgg ctccattcgt gcagcgtgcg
     6841 atggctctgg aaggtggagt agccgagcgc cagcgcgagg agcgaaagag ccgcgatccg
     6901 gccggtccgg gccgcggcgc gctcggcggc ccaggccagt cccagcgccg tcactgtggc
     6961 gacggcgggc gtcagcacca gcgtgcgcag aaagttgggc gaatcggtga tcgtgaaaat
     7021 agtcggaagc aggccggcgg cgatccagcc gaggaggatc gcgccgtgag ggcgaggcaa
     7081 gccgccggcg gagcgcagcg agcgggcgca gagcgccaga ccgatgagga aaaacaggcc
     7141 ctgaaccgga tcgagcgcgg gccgccacgg gaggttctgt ttggcgacat gatcgccggt
     7201 cagatggaac atgccgagcg cctcccaggc gttttcgaag acttcggcgg ccgccgcggt
     7261 cggcggcaga ttcgtgaccg agacctggcc ggcccgcgcc atcaggtcct cggggtgccg
     7321 gacgtaatgc acgaggagcg gaagggcgac cagcgcggcc aggccgaggc agaggcgggt
     7381 cgacgtcggc ggccggggcg cggcgccggc acggtcgcgc gacgcaatcc acagcaccag
     7441 cgggatccac agcagcgcca tgaaatacca ggcgaggtag gtgtagaacc ctccgccgat
     7501 cagcacgccg gcgagggccg cgcgccagag cgcgggacgg cgccgccagg ccaccaccgt
     7561 ccagacgaag gccgccatcc agggcgccag cagattggtg cggaagacca gccggctgaa
     7621 gagaacatgc cagaagagcg tggccagcac ggcggcggcg agcaagccga cgcgtgggcc
     7681 tgcgagcgcg cgggccatcc cgaaaacgag gaccagcgcg gcgatgccga tcagcgccga
     7741 aacgagacgc aacgtgaccg ggacggggcc caggacgcgc acggcgcagg cgagcagggt
     7801 gatgtagagc ggctcgcgcg ggtattcctc gggatagacc atgcgaaagc cctcgccgcc
     7861 gggttgccag accgcgacgg cgtcctggcc gttgagggct tcatccagcc acaggccggg
     7921 cgggatcgca ttgagatgcg tcagccgcag ccagccgccc aggcaaagga tgaccgccaa
     7981 cggcagcagc ggaccgggag ggactcgcca ggcaaagcga ttcatgggag gtgcatttta
     8041 ggacggcggg gggaggatgg cgagggggag gatgggtccg ggatggcgcg gaatgatgaa
     8101 tgaagatggg ttctcgcgtg tgccgggggg aaagatcgat taggatggga cacagcaacc
     8161 cgccccgcca gggacaagat gccggatttt cgagagacga gnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn ngggacacag caacccgccc cgccagggac aagatgccgg
     8341 attttcgaga gacgagagcc gaattctggg cgggcggttg ggggcgatca cgaggacgag
     8401 agacgagaga cgaattcttg agcgagggga cggatggttg agggtgcggg caagcgggcg
     8461 gtgatcctgg gggccggcgt ggcggggctg gcgacggcgc tgcggctggc cgagggggga
     8521 ggctggcggc cgcggctgct ggagcgcgag gaccggccgg gggggctggc gcgcagcctt
     8581 cattttcgcg gcgtttcaac cgatctgggg ccgcaccgga tccacaccga aatccccgag
     8641 gtgcaggcgc tgatcgaatc ggtggcggcg cggtcgctgg tccggctcag gcgctccagc
     8701 cacatcttcc tgcgcaagaa atggctgacc tacccgccgc ggccgctgga actgctcggc
     8761 gcgctcggcc cgatccggat ggcgcgattc gggacgggct tcgccctgga aaagttgcgg
     8821 ccgcgtccgg cgcaggaaac ctatgagagc ctgatgcggg cggcattcgg gggcccactg
     8881 tgggaattcc tgctgcggcc ctacagcgcc aagacgtgga agatcgaccc cggcgaactg
     8941 cacgccgaca cggcgcgggt gcgggtctcc gccggcagcc tgggcaagct ggtggcgggc
     9001 ctgctgcggc gcgaacagca gggggcggag acggcgctgc gggaattccg ctatgtcaag
     9061 ggcggggccg agacgctcgt gcggcatctg tgggccgccg cgcatgcggc cggggccgag
     9121 ctggaactga acaccgcggt tcaggaactg gagctggacg agcggggaag ggtccgcgcg
     9181 gcgctggcgc tgcggcggcc ggcgcatggg acggccgagg gggccgacca gcggttcgag
     9241 ggcgagttgt tcatctcgac cgtgccgctc ccgatcctgc tgggccggct gctgccgcaa
     9301 cgggccgagc tgatggcggc gcgggaggcc gcggcgcggc tggaatatct ggatatggcg
     9361 ttcgtctgcc tgatcgtgcg gcgcaaggtg atcaccggcg acaactggct ctactttccc
     9421 gagccgcacc tgatcttcaa tcgcggctac gaggcgaaga gcttcgaccc ggcaatggcg
     9481 cccgaggata agtcggtgat ctgcctggag atcacgatgc gccggggcga tgcgctggcg
     9541 cgcgaatccg acgcgctgat cctgcaaacg gcgaccgaac atatcgtttc gaccggcctg
     9601 ttccgcaagg aggagat
//
LOCUS       DAOPCO010000152         9530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6824_length_9530_cov_5.060686, whole genome shotgun sequence.
ACCESSION   DAOPCO010000152 DAOPCO010000000
VERSION     DAOPCO010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6824_length_9530_cov_5.060686"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLS90_10975"
     CDS             complement(<1..544)
                     /locus_tag="PLS90_10975"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPK02967.1"
                     /translation="MILNDFRSLLARQPALPAPMCGISDFPWRELCRRMGARVTYTQM
                     VSAEALWRGDAKTLEILDLAPGETHVAMQLFGGEPEALAHACRVLQDHGALLIDLNMG
                     CPARKITCSHAGSALLNDLPRVARIFRAMRQATRVPLTAKMRWDWADGLTPADALNDK
                     QRAGAALAVARLAEAEGLDGV"
     gene            909..1478
                     /locus_tag="PLS90_10980"
     CDS             909..1478
                     /locus_tag="PLS90_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPK02968.1"
                     /translation="MHINDSSAWRAFEESELTHSAAHYLMTIRHLKQQQGYARVTDVA
                     EHLQVSRGAASRATTLLKERGWIQEDPHRMLEVTESGQELARMVERNFLVVECFLEDI
                     LGVPREVAYEDACKLEHLLSPATTETLFKLVRVLQEDKPLHRKLLARLQNREIPCNGE
                     GNCEICTEYEGCIAEDAAHVRHERQSSAG"
     gene            complement(1605..2042)
                     /gene="mnhG"
                     /locus_tag="PLS90_10985"
     CDS             complement(1605..2042)
                     /gene="mnhG"
                     /locus_tag="PLS90_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPK02969.1"
                     /translation="MIEVLSTLFALLGAFFITVASIGILRMPDLFLRMSATSKASTLG
                     SALTLIAAMIFFQDFSVTTRCLATILFLILTTPVAAHMIGRAGYLSGVRLWERTITDE
                     LAGHYDAEARRLEGAAPTPSPAEPQPEKTSETDRFSPPSGRPE"
     gene            complement(2039..2335)
                     /locus_tag="PLS90_10990"
     CDS             complement(2039..2335)
                     /locus_tag="PLS90_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013409673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPK02970.1"
                     /translation="MIDPIPHPAGLAGLSYLLILGMLGVAVVLTFIRLYRGPKLADRV
                     IALDLLAIESIGIIVNYAMALNRQMLIDVAIVLALLAFLGTVAFARYLEKRVEP"
     gene            complement(2332..2799)
                     /locus_tag="PLS90_10995"
     CDS             complement(2332..2799)
                     /locus_tag="PLS90_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPK02971.1"
                     /translation="MFVLNLILALVWAALTADFSPLNLFVGYVGGYFILYMLRRVESP
                     SPYFRRLPRILRLIAIFLRELVVANLRVCYDVLTPTHYMKPAILAIPLDLESDEEITL
                     LANLITLTPGTLSLDISTDRKVLYIHAMYVDDLDQARREIKQGFEREVREVFR"
     gene            complement(2803..4329)
                     /locus_tag="PLS90_11000"
     CDS             complement(2803..4329)
                     /locus_tag="PLS90_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HPK02972.1"
                     /translation="MAATGVHVLLLCPVLIPLLGAIFSIFGWNRRRYQRTVLVVGMAL
                     LLAAAVALLRVVSAEGIQSVQVGNWPAPFGITFVADLFSAIMVLLGAMMGLAVAVYSF
                     ISIDVRRESFGYYPLFNTLMMGVSGAFLTGDLFNMYVWFEVMLISSFVLLALGGERAQ
                     LEGSIKYVTLNLLSSAVFLAGVGILYSVVGSLNMADIARLMSARVTEGIALTIAMMFL
                     IAFGIKAAIFPMFFWLPASYHTPPVAVSAIFAGLLTKVGVYSLIRVFTLIFVQNPGYT
                     HGAIAILAGFTMICGVLGAAAQMEFRRVLSFHIISQIGYMILGLGFFTPLALAGSVFY
                     LIHHIIVKTNLFLVSGVVHRLQGTYQLKPLGGLYKRHPLLGLLFLIPAMSLAGVPPLS
                     GFWAKFILIREGLELRHYLLVAAALVTSILTLYSMSKIWVEVFWKDKPESRVFGGAPI
                     AHRLPRIVWVLYLAPIVALALCTVIIGLAAEPVFALSLRAAEQLMDPQAYIEAILGGG
                     "
     gene            complement(4329..4694)
                     /locus_tag="PLS90_11005"
     CDS             complement(4329..4694)
                     /locus_tag="PLS90_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HPK02973.1"
                     /translation="MQAVLILTVGILYAAGIYMMMRRSIVKLIIGLGLLGHAANLLIF
                     TAGGLRRGAPAFIDPDQSAAALAQIADPIPQALILTAIVIGFGVQAFSIVLIRRVYQV
                     NGNDDLKHMRSTDELGSNP"
     gene            complement(4696..5103)
                     /locus_tag="PLS90_11010"
     CDS             complement(4696..5103)
                     /locus_tag="PLS90_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit B"
                     /protein_id="HPK02974.1"
                     /translation="MPSLILNTATRYLMPLLVTFSIFVLLRGHNLPGGGFIGGLIAAA
                     AFVLYAIAHHVAAARRALPLDPLQLIALGLLTAVSSGILSIVAGEPFMTGLWSHDPWP
                     VLGAPGTPLLFDIGVYLVVLGVALTIVFALAEE"
     gene            complement(5127..7445)
                     /locus_tag="PLS90_11015"
     CDS             complement(5127..7445)
                     /locus_tag="PLS90_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative monovalent cation/H+ antiporter subunit
                     A"
                     /protein_id="HPK02975.1"
                     /translation="MTLLAVLSPFALAIVAPWLQRRLDRAAGWLLAALPAVLFIGFLN
                     FALRHPAESHVFSWPWIPSLQIQLSFLLDGLSLLFALMITGIGALVLIYSGGYLAGHP
                     HLGRFYVYLLMFMGSMLGVVLSNNVLSLFVFWELTSFSSYLLIGFYHDRQASRAAALQ
                     ALLVTGAGGLALLAGVILLAFAGGSWELSELLARGDLVRGHPLLHATLVLILLGAFTK
                     SAQFPFHFWLPAAMEAPAPVSTYLHSATMVKAGVYLLARLHPILGAAPRWQLYVGGCG
                     ALTMLVGAGLALLQVDIKRQLAYTTVSVLGALTFLLGLGGETAVTGAILYVLVHSLYK
                     GALFLVGGNVDHAVDSRDVTRLRGLRRAMPITFVAALLAGLSMAGLPPFVGFISKELI
                     YEALHAHDLSLLLAAVAVAANGCMFVVALLIGVRIFVGPPAATPREPHEAPPSMWLGP
                     LALASLGLIFGLVPGLIEGRLIAPAVAAVGQLPATPAPHLALWHGFTPMLLLSAVTIA
                     AGLLVFLGWDFLRARLNRLAPLGRLGPARWYEWVLQGVLGFAHLQTRILQSGSLPVYI
                     ATLIVVIILLVGGTLLEQVQIVRLANWNDIRFHELCIILTMMMAILTLVTTNSRLTAI
                     AALGVVGYCVSLIFVLFGAPDLAITQFCIETLSVVLFVLVLYKLPRFQVFSRWPTRLA
                     EAAIAVAFGLVVIGLMTLGMESDLGSPISEFYAANSYLQAHGRNIVNVILVDFRGVDT
                     MGEITVLAIGALGVLALLKLRLDRDRPGKESS"
     gene            complement(7716..>9530)
                     /locus_tag="PLS90_11020"
     CDS             complement(7716..>9530)
                     /locus_tag="PLS90_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02976.1"
                     /translation="EEWEPVGDNSTLRTYRDGVLLREITIPGLWNPTGHSIRIGASPR
                     SPLYDDFGAPPNAVFSRIRVYSVACAPPAAPTLTAPAANETLPSATVGALWEGDAHSH
                     VQLRITREPDLDSELLWDSGLLESPNDWIWSGKLPQQTPAYAAVRLGNLCGVGPWSEP
                     VGPFNLDRGYEPQEADTVRLVGAVLVDNQGPFLGLGASYFQAMRRCKYDRDRFESDLS
                     FLAANEFRYIRILSMVGWYEAWQGLEIAPVSFNNRANVPVAAWEDYWQQFRDCIDIAY
                     EHGLRTEVTIFADAQMMPDKADRIAHMDTMLENLVGREHKVILLEVGNEAWQNGFSGA
                     QGRADLREFGQYLAERTEIPVALSAPDDISDAGINLLYSGSAADIATVHFTRDLAPDA
                     GWAPVYDIYRTGLVDVPPMSSNEPIGPGASVNSENDPVKLVMAAVYAWAAGLPMYVFH
                     CEAGVFGSSTFEGTPAIDAYDHLDDILPPDLSGWARNNGIMPDSPFITYCEGQPDTPW
                     SAVPGANDGVIRNNGATNGCDFIALPIAVLADGVELEPRRPMALNVHHPLTGEIVDTK
                     TLAAGERFTLPQGPGAWILRGRYLDCAVGTGVGDWTRY"
BASE COUNT         1844 a         2944 c         3420 g         1322 t
ORIGIN      
        1 agacgccgtc gagcccttcg gcctcggcca ggcgggcgac ggcgagcgcg gcgccggcgc
       61 gctgtttgtc gttgagcgca tcggcggggg tcaggccgtc ggcccagtcc cagcgcattt
      121 tggcggtgag ggggacgcgg gtggcctggc gcatggcgcg gaaaatgcgc gcgacgcgcg
      181 gcagatcgtt gagcagggcc gagccggcat gcgagcaggt gatcttgcgc gccgggcagc
      241 ccatgttcag gtcgatcagc agcgccccgt ggtcctggag gacccggcag gcgtgggcca
      301 gcgcctcggg ctcgccgccg aagagctgca tggcgacgtg ggtctcgccg ggcgccaggt
      361 ccaggatctc cagggtcttg gcgtcgccgc gccagagcgc ctcggccgag accatctggg
      421 tgtaggtgac ccgggcgccc atgcggcggc acagctcgcg ccaagggaag tcgctgatgc
      481 cgcacatcgg cgcgggcagg gcgggctggc gtgccagcag ggagcggaag tcgtttagaa
      541 tcatggcttt ccgtggctgg agagaccgca ctggccgagc cgggcggatc agccgggctc
      601 gggtcggcga agcggagcgg ttcgccgcgg cgcggactta tgctaaaata ccccagcggg
      661 ccgaatcccg ccagtcggtt tcgccgggcg caccggcggc ggcaaaggag cttgcgacgg
      721 tcggcctggt gagtgtagcc ttggatcaga cgagccagac cccccctcgg cgtggtgggt
      781 cgaggtgtcg gccaggagtt aatcccgaat agaaaaggaa tgcgttgcct gagcccatct
      841 ttgtagcaga cgctacgact tccggtggac ggcccgcttc cccccgtgaa ccgcaagagg
      901 cccgtgacat gcatatcaac gacagttcgg catggcgcgc gttcgaagag agcgagctga
      961 cgcactcggc ggcgcattat ctgatgacga tccgtcacct gaagcagcag cagggctatg
     1021 cgcgggtgac cgatgtggcc gagcacctgc aggtctcgcg gggggcggcc tcgcgggcga
     1081 cgacgctgct caaggagcgc ggctggatcc aggaagaccc ccaccggatg ctggaggtga
     1141 ccgaatcggg ccaggagctg gcgcggatgg tcgagcgcaa cttcctggtg gtggagtgtt
     1201 tcctggagga catcctcggc gtgccgcgcg aggtggccta cgaggacgcg tgcaagctgg
     1261 agcacctgct gtcgccggcg acgaccgaaa cgctgttcaa gctggtgcgg gtgctgcagg
     1321 aagacaagcc gctgcaccgc aagctgctgg cgcggctgca gaaccgcgaa atcccgtgca
     1381 acggcgaggg gaactgcgag atctgcacgg aatatgaggg ctgcatcgcg gaagacgccg
     1441 cgcacgtgcg ccacgaacgc caatcctcgg ccgggtagcg gttcccgcca ctgaagtagt
     1501 tgtcattccc ctaatttcag accaactccg accacccctt tcaggggctt ggatcgcagg
     1561 gggcgggcac aaaagatctc ggcctgcggc ctccaccctg cgctttactc cggccttccg
     1621 ctcggcgggc tgaaccgatc cgtctcgctg gtcttttccg gctggggctc cgcgggcgat
     1681 ggggtgggcg ccgcgccctc cagccggcgc gcctcggcat cgtagtggcc ggccagttca
     1741 tcggtaatcg tgcgctccca gagccggacg ccgctcagat agccggcgcg gccgatcatg
     1801 tgcgcggcga cgggcgtggt caggatcagg aacaggatcg tggcgagaca gcgcgtcgtc
     1861 accgagaaat cctgaaagaa aatcatcgcg gcgatgagcg tgagcgccga gccgagcgtg
     1921 gaagctttgc tggtggccga catgcgcagg aacaggtcgg gcatgcgcag gatgccgatg
     1981 ctggcgaccg tgatgaaaaa cgcgcccagc agggcgaata gcgtgctgag gacctcgatc
     2041 atggttcgac gcgtttctcc agatagcggg caaaggcgac tgtgccgagg aaggcgagca
     2101 gcgccagcac gatggcgaca tcgatcagca tctggcgatt gagggccatc gcgtaattga
     2161 cgatgatgcc gatcgactcg atggcgagca gatcgagggc gatgacgcga tcggcgagct
     2221 tgggcccgcg gtacaagcgg atgaacgtca gcacgacggc aacgccgagc atgccgagga
     2281 tcagcaggta ggagaggccg gccagtccgg cggggtgggg gatcgggtca atcaccggaa
     2341 cacctcgcgc acctcgcgtt cgaatccctg cttgatctcg cggcgcgcct ggtccagatc
     2401 gtcgacgtac atcgcgtgaa tataaagaac cttgcgatcg gtcgagatgt caagcgagag
     2461 cgttccgggc gtcagcgtga tcaggttggc cagcagcgtg atttcctcgt ccgactccag
     2521 atcgagcgga atggcgagga tcgcgggctt catgtaatgc gtcggcgtca gcacgtcgta
     2581 gcagacccgg aggttggcga cgacgagctc gcgcagaaag atggcgatca gccgcaggat
     2641 gcgcgggagc cggcggaagt agggcgatgg gctttcgacg cggcgcagca tgtagaggat
     2701 gaagtagccg ccgacgtagc cgacgaacag gttgagcggg gagaagtcgg cggtcagcgc
     2761 ggcccacacc agcgcgagga tcagattgag gacgaacacg gttcagcctc ctccgaggat
     2821 ggcctcgatg taggcctgag gatccatgag ctgttcggcg gcgcgcagcg agagggcgaa
     2881 gaccggttcg gccgccaggc cgatgatcac ggtgcacagg gcgagggcga caatcggcgc
     2941 gaggtagagc acccagacga tgcgcggcag gcgatgggcg atcggcgcgc cgccgaagac
     3001 gcgcgactcg ggtttgtcct tccagaagac ttcgacccag attttcgaca tcgaatagag
     3061 cgtcaggatg ctggtgacca gcgcggcggc gaccagcagg taatgccgga gctcaagccc
     3121 ctcgcggatc aggatgaact tggcccagaa ccccgagagc ggcggcacac cggcaagcga
     3181 catcgcgggg atcaggaaga gcagtccgag cagcggatgg cgcttgtaga ggccgcccag
     3241 cggcttgagc tgataggtgc cctggagccg gtggaccacg ccgctgacca gaaacaggtt
     3301 ggtcttcacg atgatgtgat ggatcagata gaagaccgag ccggcgaggg cgagcggcgt
     3361 gaaaaagccg aggccgagga tcatgtagcc gatctggctg atgatgtgga acgagagaac
     3421 gcggcggaac tccatctggg cggcggcgcc cagcacgccg cagatcatcg tgaagccggc
     3481 caggatcgcg atggcgccgt gcgtgtagcc ggggttctga acgaagatca gcgtgaagac
     3541 gcggatcagc gaatagacgc cgaccttggt gagcagcccg gcgaagatcg ccgaaacggc
     3601 gacggggggc gtgtgatacg aagccggcag ccagaagaac atcgggaaga tggccgcctt
     3661 gatgccgaag gcgatgagga acatcatcgc gatggtcagg gcgatccctt cagtcacgcg
     3721 cgcgctcatc aggcgggcga tgtcggccat gttgagcgac ccgacgacgc tgtagaggat
     3781 gccgacgccc gcgaggaaga cggccgagga gagcagattg agcgtgacgt atttgatcga
     3841 gccctcgagc tgggcgcgct cgccgcccag cgccagcagg acgaacgagg agatcagcat
     3901 cacctcgaac cagacgtaca tgttgaagag gtcgccggtg aggaacgcgc cgctgacgcc
     3961 catcatcagt gtgttgaaca gcgggtagta gccgaagctt tcgcggcgga cgtcgatgct
     4021 gatgaacgaa tagacggcga cggccaggcc catcatcgcg ccgagcagga ccatgatcgc
     4081 gctgaacaga tcggcgacga acgtgatgcc gaacggggcg ggccagttgc cgacctgaac
     4141 cgactggatg ccctcggccg agacgacgcg cagcagggcg acggcggccg ccagcagcag
     4201 cgccatgccg acgacgagca ccgtgcgctg atagcggcgc cgattccagc cgaaaatgga
     4261 gaagatcgcg cccagcagcg ggatcagaac ggggcagagc agcaggacgt ggacgccggt
     4321 cgccgccatc aggggttgct ccccagttcg tcggtcgagc gcatgtgttt caggtcgtcg
     4381 ttgccgttga cctgatagac gcgccggatc aggacgatgg agaaggcctg gacgccgaag
     4441 ccgatgacga tggcggtcag gatcagcgcc tgggggatcg ggtcggcgat ttgcgcgagc
     4501 gcggcggcgg actggtcggg atcgatgaag gcgggcgcgc cgcgccgcag gccgccggcc
     4561 gtgaagatca gcaggttggc cgcatgcccc agcaggccga gtccgatgat cagcttgacg
     4621 atgctgcgcc gcatcatcat gtagatgccg gcggcgtaga ggatcccgac ggtgaggatc
     4681 aggacggcct gcatgctatt cctccgccag ggcgaagacg atggtgagcg cgacgccgag
     4741 gacgaccagg tagacgccga tatcgaacag cagcggcgtg cccggagcgc ccagcacggg
     4801 ccaggggtcg tgcgaccaga ggccggtcat gaacggttcg ccggccacga tggacaggat
     4861 gccgctggag acggcggtga gcagcccgag cgcgatgagc tggagcggat cgagcggcag
     4921 ggcgcggcgc gcggcggcga cgtgatgggc gatcgcatag agcacgaagg ccgcggccgc
     4981 gatcaggccg ccgatgaagc cgccgccggg cagattgtgc ccccgcagca ggacaaagat
     5041 cgaaaaggtg accagcagcg gcatcaggta gcgcgtggcc gtgttgagga tcagcgacgg
     5101 catcatcgag cgggcgggcg gcgggttcat gaggactcct tgccggggcg gtcgcggtcc
     5161 aggcgcagct tgagcagcgc gaggacgccg agcgcgccga tggccagcac ggtgatctca
     5221 cccatcgtgt cgacgccgcg gaaatcgacg aggatcacgt tgacgatatt gcggccgtgc
     5281 gcctgcagat agctgttcgc ggcatagaat tcagagatgg gcgagcccag gtcggactcc
     5341 atgccgagcg tcatcagccc gatgacgacc aggccgaagg caacggcgat ggcggcctcg
     5401 gccaggcgcg tcggccagcg cgagaagacc tgaaaccgcg gcagcttata cagcacgagg
     5461 acgaagagaa cgacgctgag cgtttcgatg cagaactgcg tgatcgccag atcgggggcg
     5521 ccgaacagga cgaaaatgag cgagacgcag tagccgacga cccccagcgc ggcgatggcc
     5581 gtcaggcgcg aattggttgt gaccagcgtc agaatggcca tcatcatcgt cagaatgatg
     5641 cacagctcgt ggaagcggat gtcgttccag ttcgccaggc ggacgatctg gacctgctca
     5701 agcagcgtgc cgccgaccag caggatgatc acgacgatca gcgtggcgat gtagaccgga
     5761 agcgagccgc tctggaggat gcgcgtctgc agatgggcga agccgagcac gccctgcagc
     5821 acccattcat accagcgggc gggtcccagc cggccgaggg gcgccaggcg gttgagccgc
     5881 gcgcggagga aatcccagcc caggaagacg agcaagccgg ctgcgatggt gacggcgctg
     5941 agcagcagca tgggggtgaa cccgtgccac agggccaggt gcggcgcggg cgtcgccggc
     6001 agttgcccga cggcggcgac ggccggtgcg atcagccggc cctcgatcag tccggggacc
     6061 aggccgaaga tcagccccag actcgccagc gccagggggc cgagccacat cgacggcgga
     6121 gcttcgtgcg gctcgcgcgg cgtggcggcc ggcgggccga cgaaaatgcg gacgccgatc
     6181 agcagcgcca ctacgaacat gcagccgttg gccgcgacgg ccaccgcggc gaggagcaac
     6241 gacagatcgt gggcgtggag cgcctcgtag atcaactcct tgctgatgaa gccgacgaac
     6301 ggcggcaacc cggccatcga gagaccggcg agcagcgcgg cgacaaaggt aatcggcatg
     6361 gcgcgccgca ggccgcgcag ccgggtgacg tcgcgcgagt cgacggcgtg gtcgacgttg
     6421 ccgccgacga ggaacaacgc ccccttgtac agcgaatgga cgagaacgta gaggatggcg
     6481 cccgtgacgg cggtttcgcc ccccaggccg agcaggaacg tgagggcgcc cagcacgctg
     6541 acggtcgtgt aggcgagctg gcgcttgatg tcaacctgga ggagcgcgag cccggcgccg
     6601 accagcatcg tcagcgcgcc gcaaccgccg acataaagct gccagcgcgg cgcggcgccg
     6661 aggatcgggt ggagccgcgc cagcagatag acgccggcct tgaccatcgt ggccgagtgc
     6721 agatacgtgc tgacgggcgc gggggcttcc atcgcggcgg gcagccagaa gtggaacgga
     6781 aactgcgccg atttggtgaa cgcgccgagc agaatcaaca ccagcgtggc gtgcaggagt
     6841 ggatggccgc ggacaaggtc gccgcgcgcg agcaattcgc tcaactccca gcttccgccg
     6901 gcgaacgcca gcaggatcac gccggccagc agcgccagcc cgccggcccc cgtcaccagc
     6961 agggcctgca gcgcggcggc gcgcgaagcc tggcgatcgt ggtaaaagcc gatcagcaga
     7021 taggagctga agctcgtcag ctcccagaag acgaacagcg agaggacatt attcgagagc
     7081 acgacgccga gcatcgagcc catgaacatc agcagataaa cgtaaaagcg gcccagatgg
     7141 ggatgaccgg cgagatagcc gcccgagtag atgaggacca gggcgccgat gccggtgatc
     7201 atgagcgcga agaggaggct cagcccgtcg aggaggaacg acagctggat ctggaggctg
     7261 ggaatccagg gccaggagaa gacatgggat tctgccggat ggcgcagggc gaagttgagg
     7321 aacccaatga acaggacggc gggcagcgcg gccagcagcc agccggcggc gcggtccaga
     7381 cggcgctgaa gccagggggc cacgatggcc agcgcaaacg gcgacagcac ggcgaggagc
     7441 gtcatgacgc ggggggtccg ccgcaggccg ccgccgcggc gcgacgtgga tgcgacggcg
     7501 cggcggccag ccgcgcgccg cggaagaggt tcaagagttg catcagcctt ctccggcgcc
     7561 tacctcggcc agcgtcgggc gcgggcaggg aaagaaggca tggtactgga aatcagccgg
     7621 cgaaggaacg aaaaccggac cggcgatacg gccagtgtgg gagcggcgcc cccgccggtc
     7681 catcccacca gcatcattcc gcgcctggtt gccgcttaat atcgcgtcca gtcgccgacg
     7741 cccgtcccga cggcgcaatc gagataccgg ccgcggagga tccatgctcc cggcccctgg
     7801 ggcagcgtga accgctcgcc ggccgcgagc gttttcgtgt cgacgatctc gccggtcaac
     7861 ggatggtgga cgttaagcgc catcggccgg cgcggctcca gctcgacccc gtcggccaac
     7921 accgcgatcg gcagcgcaat aaaatcgcag ccgttcgtcg ccccgttgtt gcggatcacg
     7981 ccgtcgttcg cccccggcac cgccgaccac ggcgtgtcgg gctgaccctc gcagtaggtg
     8041 atgaacggcg aatcgggcat gatcccgtta ttgcgggccc agcccgacag atccggcggc
     8101 aggatgtcgt ccaggtgatc atacgcatcg atggcgggtg tcccctcgaa cgtggagctt
     8161 ccgaaaacgc ccgcctcaca gtggaagaca tacatcggca ggcccgccgc ccaggcgtag
     8221 accgccgcca tcaccagctt gacgggatcg ttctccgagt tgaccgacgc ccccggtccg
     8281 atcggttcat tgctgctcat cggcggcaca tcgacgaggc ccgtgcggta aatgtcgtag
     8341 accggggccc agccggcatc gggcgcaagg tcgcgggtga agtggaccgt cgcgatgtcg
     8401 gccgccgagc ccgaatacaa caggttgatg ccggcgtcgc tgatatcatc cggcgcgctg
     8461 agcgccaccg ggatctccgt ccgctccgcc agatactggc cgaactcgcg caggtcggcg
     8521 cggccctgcg ccccggagaa gccgttctgc caggcctcat tgcccacctc cagcagaatg
     8581 accttgtgct cccggccgac caggttctcc agcatcgtgt ccatatgcgc gatgcggtcg
     8641 gccttgtccg gcatcatctg ggcgtcggcg aagatcgtca cttcggttcg caggccgtgt
     8701 tcataggcaa tgtcaatgca gtcgcggaac tgctgccagt agtcctccca tgcggccacg
     8761 ggcacgttgg cccgattgtt gaagctgacc ggggcaattt ccagaccctg ccacgcctca
     8821 taccagccca ccatcgagag gatgcggata tagcggaatt cgttggcggc gaggaagctc
     8881 aggtcgctct caaaccggtc ccggtcgtac ttgcaccggc gcatcgcctg gaaatacgac
     8941 gcgcccaggc ccagaaacgg tccctgatta tccacgagca cggcgccgac caaccgcacc
     9001 gtatcggcct cctgcggctc atacccgcgg tcgaggttga acggcccgac gggctcgctc
     9061 cacggaccca cgccgcacag gttgcccaga cgcaccgccg cgtaggccgg cgtctgctgc
     9121 ggcagtttcc cgctccagat ccagtcgttg ggcgattcaa gcaggcccga atcccacagc
     9181 agctcggagt ccagatcggg ttcgcgcgtg atccgcagtt gaacgtgcga atgcgcgtcc
     9241 ccctcccaga gcgcgccgac cgtcgccgag gggagcgttt cattcgccgc cggggcggtc
     9301 agcgtcggcg cggccggggg cgcgcacgcg acgctgtaga cccggatgcg actgaagacc
     9361 gcattgggcg gagccccgaa atcgtcatac aacggcgagc gcgggctcgc gccgatccgg
     9421 atgctgtggc cggtcggatt ccacaggccg gggatcgtga tctcgcgcag cagcaccccg
     9481 tcgcgatacg tccgcagcgt gctgttgtcg cccaccggct cccattcctc
//
LOCUS       DAOPCO010000153         9355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_6969_length_9355_cov_5.095484, whole genome shotgun sequence.
ACCESSION   DAOPCO010000153 DAOPCO010000000
VERSION     DAOPCO010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_9355_cov_5.095484"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            790..2376
                     /locus_tag="PLS90_11025"
     CDS             790..2376
                     /locus_tag="PLS90_11025"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK02977.1"
                     /translation="MEPPLIRLDRVVKRFGRRRVLDGLSWELRAGECAVILGPSGGGK
                     TVFLHTLLGLHAPDEGQVDRAGLGPDPFRAMAVMFQEDALLDDRTVAVNLATALLERA
                     DLFEPPADPAARAAIDAVLREVDLNPETDGPKLPSALSGGMRRRVALARALIRRPRLL
                     IADEPTTGLDPTSSALIYDLLGRLIRQRGMSAVIITHDPACASRLGYPVYFFSPVEGT
                     MPRWTPPDPGRRDDLGAHDEQRHHALQLWMRDQLDSHRRRRSEAGQAKEPAADLPTEP
                     AGAAGAARFINDALEALGRSALLLARLAGRFSPALLLRGLRDWGLGSLPLVALIFLLL
                     GVVMQVQAESAVVDYGLSNRLPELVALSLLRLSPILTGFLLAGRCGSAIGAHVGFMEL
                     SGQRRALWTMRIDPDRTLFPPLFWSLTLAAPLLALIGMAAGAAAAIALLGLPVSRAQI
                     TPTFFLRRFPTFLSAGELGVLVVKSMLMGGGLAAIAYGLGARPKRSPADVTRAITLTL
                     VLAFVWMTLVDAALSLLFPN"
     gene            complement(2603..4036)
                     /locus_tag="PLS90_11030"
     CDS             complement(2603..4036)
                     /locus_tag="PLS90_11030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPK02978.1"
                     /translation="MLINPPTGLYRRDDRCQCKVEDQTVQIIFPPVELATLAATLRRG
                     GAEVELRDYPAQSAGWEDYIEDLRRLRPDMVLVNVVTATSEGDFDALRAAKETLGADN
                     VLTIAKGEYMEALGESVLRHRPEVDFGFHGEIDLVLDQLYKGEPLETIDGLIWRRAPA
                     NGHPADVVRNPGHPLIEELDALPFPARDLLRNDLYRSPETNRPLTVIHGNRGCPAKCI
                     FCPAGVMSGYRIRYRSPGNIMAEIDDCVRTYGIREFLFHGDTFTINKKWLIELCDLII
                     ASGHKIRWGCNSRVDTIDDERAAKMRQAGCWVVAFGVESGNQEILDYMKKGQKAERAF
                     KAAEVCRRHGLRVHTFFVIGTPLETRATLRETYEYARKIDPDFFDFNIAYPLPGTELY
                     EIAEREGLFEKPPDATGYANAAVRTRELSSRDLTEWRRKALLSMYLRPRYIARMLYRA
                     GSPRVALNYVRAGARRFRQLISPAPDS"
     gene            complement(4240..6756)
                     /locus_tag="PLS90_11035"
     CDS             complement(4240..6756)
                     /locus_tag="PLS90_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02979.1"
                     /translation="MAGLGQVGRVVCQVGLGLVLAAGGVARAQTDPVFITPVDDAQLT
                     SPALTFEWQVDLATLTYVMRIEEIDAEGAVVPGTPACEFVFAQGDTTNLGSNRRRLVY
                     NLESPPAFVGAEERADLATLGSGRFRVTLNALQLADDGEGVTTYTFGAPAPQIEMSTE
                     DAKDPRDLRWRLRADDRDNTPEYRYTLTFAPQGSGEPVPLVMESAAEAVDQAGLGIAN
                     TATALGLMEGTTYDLQLLVEDTLNQSAAAGLGASGTAEGMVRINDLARITARPDDFIF
                     HYPHGDVWAATVPAAGVMATDLDSAAVDIEAVIDYPGGDDPGAQFFPARAVDTSHTLE
                     LDLTAHDPAQPHPMYGVQLRATDALEVTGELSEPFSVTLNQRPAVLSAEVLPAKQIDY
                     DGSTDTATVPVTLRVTVDDFETETLDVRVAIHDPADDTLRDTQTLEDVPRNVATEREI
                     LLPKSPPAEDYAEYLLRITALDETEAGDIIESAASDVAEIDHVRLNEIPHAVEAPTAP
                     AVVSYDGTPALVQVPLTVHAGDKEPGPLTVYVDARSTDGELLETINVGAIEQDVESVV
                     NLEIAPRAPRRIYDLEIYFRDEAGADSFRTTRSVRLNQPPGLAAQHAPAAAERQDLAA
                     DAETIPFDVELTDDWSTSLTIQFEGLGALQSFDFERDFIAQVERVEGTLAFDAFGAHA
                     VTETIRDGDGATAVRDLTPLVINDLPMLLLSVTPAIFDAQTGTWRVEQSRPTTFAAQG
                     RNIHRDPDELLTLTRQLETSDEPGLKELASGNPPAAGIVHELHGSETRQFEELGEFSY
                     DLTLTERFDGEEVEVTASFPIRVEESIPELNAAGDWRLYR"
     gene            complement(7627..8136)
                     /locus_tag="PLS90_11040"
     CDS             complement(7627..8136)
                     /locus_tag="PLS90_11040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPK02980.1"
                     /translation="MTDTIYSVPVEPMADDDPATTETLAPWQGKVLLLVNVASRCGYT
                     PQYAGLEQLHRAYFDQGLRVLGFPCNDFGGQEPGTEAEIVEFCQTNYDVTFPLYGKLH
                     AKGEEISPLFEYLTGDKSPHPGEVKWNFEKFLIGRDGRLVARYASAVTPDAPELIEAI
                     EAELAKPAP"
     gene            complement(8234..8950)
                     /gene="nth"
                     /locus_tag="PLS90_11045"
     CDS             complement(8234..8950)
                     /gene="nth"
                     /locus_tag="PLS90_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPK02981.1"
                     /translation="MPSPRPPKPAASPLRRTPAPELKARARRISTRLRRHYPDAGTAL
                     HFDGPFQLLIATILSAQCTDEAVNRVTPELFQRYRDPAAFAAADAAEIEKLIHSTGFF
                     RQKTRSIQNCSRALLERHGGRVPEAMEELTALPGVGRKTANLVRACAMGHPGLIVDTH
                     FKRVIGRLGLTAETDPDRIEFAVAELLPPSHWTHFSNALIWHGRRICTARKPRCPECP
                     VCPDCDFGRPHCTGRLTMKG"
     gene            9064..>9355
                     /locus_tag="PLS90_11050"
     CDS             9064..>9355
                     /locus_tag="PLS90_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPK02982.1"
                     /translation="MTPQRKQPRSFGSRSGFIHTRTRKQSELMEDYVELIDDLIIEKG
                     EARAVDVARCLGVSHVTVSKTIGRLKEAGLVRAQPYRSIFLTESGREMAQASR"
BASE COUNT         1506 a         3446 c         2776 g         1627 t
ORIGIN      
        1 atcccttctc cccccatccc cccctccgcc tcatggaaat aagcaaccgg ccgagacctg
       61 ggagcgccac tcttctgagt gctttcgcct caattcttat cccccatcgc aatccccccg
      121 aacccaacat cccgtcccag acccatcctc cgcgcatgca cctctccgcg tctcagcgtg
      181 agattatcgc ccccaacggc ccacccagga attcttcccc ttctctcctt cctcaccttc
      241 gtctcgcgag aagacccttc aagaacccct gccggtgtcg aaccaccgcc ctcgccggtc
      301 ccctctagag tccgcggagc ggacgagtgg aataaagcgc agggtggagg ccgcaggccg
      361 gaaccctgcg tctccgcccc caacgccaca aacccctgca aggggtggcc ggagattgcg
      421 gcgtgcaatc caatcacgct catttcgtgt tcgtacaccg gccggtgatg gcgactttgc
      481 cggtgtggga ccggccccct cgtgctcgcg acggcaagaa ttcaccgacc aacccacgct
      541 ccccctctcc gtacccagcc aattcagcaa ccgcgcccgc ggttcgatat ccatgccgtg
      601 ggttgcaatg gccccccggc gcctatgcaa cccacggtcg gggcaaccac cagccccaaa
      661 ccgattgatc gattcgataa ttccaatatc aatccaccgc cgcgactcct cacaatcgcc
      721 ctgtcccccc gcacctccgc cccccattcc ccctcgcatt cccctccgcc tcacactatc
      781 gtgatctcca tggaaccgcc gctgattcgc ctcgaccgcg tggtgaaacg cttcggccgc
      841 cggcgcgtgc tcgacggcct gagctgggaa ctgcgcgccg gcgaatgcgc cgtgatcctc
      901 ggcccgagcg gcggcggcaa gaccgtcttc ctccacacgc tgctcggcct gcacgcaccg
      961 gacgaagggc aggtcgaccg cgcgggcctg ggccccgacc cgttccgcgc gatggccgtc
     1021 atgtttcagg aggacgctct gctcgacgac cgcacggtcg cggtcaacct ggcaaccgcg
     1081 cttctggagc gggccgacct gtttgaaccc cccgccgatc ccgccgctcg cgccgccatc
     1141 gatgccgtgc tgcgcgaggt ggacttgaac cccgaaaccg acggccccaa gctcccctcg
     1201 gccctctcgg gcgggatgcg ccgccgcgtg gccctcgccc gcgcgctcat ccgccggccc
     1261 cgcctgctga tcgccgacga accgaccacc ggactggatc ccacctcctc ggccctgatt
     1321 tacgacctgc tcggccgcct gatccgtcag cgtggcatga gcgccgtcat catcacccac
     1381 gacccggcct gcgccagccg gctcggttac ccggtctatt ttttctcgcc ggtcgaggga
     1441 accatgccgc gctggacacc ccccgacccc ggccgccgcg acgacctggg ggcgcacgac
     1501 gagcagcgcc atcacgcctt gcaactctgg atgcgcgacc agctcgactc gcaccgccgc
     1561 cgccggtccg aggcaggcca ggccaaggag cccgccgccg acctgcccac cgagccggcg
     1621 ggcgcggcgg gcgcggcgcg cttcatcaac gacgccctcg aggcgctcgg ccgctcggcg
     1681 ctcctgctgg cccgcctggc cggacgtttc agtccggccc ttctgctgcg cggcctgcgc
     1741 gactggggac tcggctccct tccgctcgtc gccctgatct tcctgctgct cggcgtcgtc
     1801 atgcaggtgc aggccgagtc ggcggtggtc gactacgggc tctccaaccg gctgcccgaa
     1861 ctggtcgcgc tcagcctcct gcggctctcg ccgatcctta ccggttttct attggctggg
     1921 cgctgcggct cggcgatcgg cgcccacgtc ggtttcatgg aactctccgg ccagcgccgc
     1981 gccctctgga cgatgcggat cgacccggac cggacgctct tcccgccgct cttctggtcg
     2041 ctcacgctgg cggcgccgct gctggcgctg atcgggatgg ccgccggcgc cgcggccgcc
     2101 atcgccctgc tcggcctgcc cgtctcgcgc gcgcaaatca cgccgacgtt cttcctgcgc
     2161 cggtttccga cgtttctcag cgcgggcgaa ctcggcgtgc tggtcgtcaa gagcatgctc
     2221 atgggcggcg ggctggcggc gatcgcctac ggcctgggcg cgcggcccaa gcgttccccc
     2281 gccgacgtga cccgcgcgat cacgctgacg ctcgtcctgg cattcgtctg gatgaccctc
     2341 gtcgacgccg ccctcagcct cctcttcccc aactaagatg ctccgtcccg cgtcctcggc
     2401 tctcgacatc ccgctactca agccgtccgc gtgagaggcg cccagtgggg cgcaatttgg
     2461 atcgccccag gtggagcgcc gcggaacccg gtggctgaca aaattccgat ggaaattgac
     2521 cacccatttc cgggtcggcg cacccgagtt ggtaaaccac gcgcggatgg agacgaagaa
     2581 ggaagcggca ttccggcgcg aatcaggaat caggggcggg gctgatgagc tggcggaacc
     2641 ggcgcgcgcc ggcgcgcacg tagttcagcg ccacgcgcgg cgagccggcc cggtacagca
     2701 tccgcgcgat atagcgcggc cgcagataca tcgacaacag cgccttgcgt cgccactcgg
     2761 tcagatcacg actgctcagt tccctcgtcc gcaccgctgc gttggcatag ccggtggcgt
     2821 ccggcggctt ctcgaacagc ccttcccgct cggcgatctc atacagctcg gtgcccggca
     2881 acggatatgc gatgttgaag tcgaagaaat ccggatcgat cttgcgcgcg tattcgtacg
     2941 tctcgcgcag cgtcgcccgc gtctccagcg gcgtgccgat gacgaaaaac gtatgcaccc
     3001 gcaggccgtg gcgccggcag acttccgccg ccttgaacgc ccgttccgcc ttctgcccct
     3061 tcttcatata gtccagaatt tcctgattcc ccgattcgac gccgaaggcg accacccagc
     3121 agcccgcctg gcgcatcttg gccgcgcgct cgtcgtcgat cgtgtcgacg cggctgttgc
     3181 acccccagcg gatcttgtgc cccgaggcga tgatcaggtc gcacagctcg atcagccact
     3241 tcttgttgat cgtgaacgtg tcgccgtgga agagaaattc ccggatgccg tacgtccgga
     3301 cgcaatcgtc gatctcggcc atgatgttgc ccggcgaacg gtaacgaatc cgataacccg
     3361 acatgacgcc ggccgggcag aagatgcact tggccgggca gccccggttg ccgtggatca
     3421 ccgtcagcgg ccggtttgtc tcgggactgc ggtacaggtc gttgcgcagc aggtcgcgcg
     3481 ccgggaacgg gagcgcatcc agctcctcga tcagcggatg gccggggttg cgcacgacgt
     3541 cggcgggatg cccgttggcc ggcgcccgcc gccagatcag accgtcgatc gtctccagcg
     3601 gctcgccctt atagagctga tcgagcacca ggtcgatctc gccatgaaac ccgaaatcga
     3661 cctcggggcg gtggcgcagc accgattcgc ccagcgcctc catgtattcc cctttggcga
     3721 tcgtcagcac gttgtccgcc cccagggttt ccttcgccgc gcgcaacgcg tcgaaatccc
     3781 cctcgctcgt cgccgtgacg acattgacca gcaccatgtc gggccgcagc cggcgcaggt
     3841 cctcgatgta gtcctcccag ccggccgact gggcggggta atcgcgcagc tcgacctcgg
     3901 cccctccccg gcgcagcgtc gccgccagcg tcgccagttc caccggcgga aagatgatct
     3961 gtaccgtctg atcctcgacc ttgcactggc agcggtcgtc gcgccggtac agcccggtgg
     4021 gggggttgat caacaacgcg cgttggaatc ggcgagcgaa cacggcggcc ctggcggttt
     4081 cggacggaag tcttcggcga aaggcgagcc cctttgccat atttcacttt atcatttccc
     4141 gccgcaactg acaagccacc ccgcgcctgt cgttatcgct atcggcgccg ctctcgccat
     4201 cggtcacggc accggcatcg ccggcgcccg cacgccgaat catcgataca accgccaatc
     4261 cccggcggcg ttgagttccg gtatcgactc ctcgacccgg atcgggaaac tcgccgtcac
     4321 ctcgacttcc tctccgtcga accgctccgt cagcgtcaga tcgtagctga attcgcccaa
     4381 ctcctcgaac tggcgcgtct cgctcccgtg aagctcgtga acgattccgg cggcaggcgg
     4441 attccccgac gccagttctt tcagccccgg ttcgtccgac gtctcgagct ggcgcgtcaa
     4501 cgtcagcagc tcatccgggt cgcgatgaat gttccgcccc tgcgcggcga acgtcgtcgg
     4561 ccgggactgc tcgacgcgcc acgtgccggt ctgcgcatcg aaaatcgccg gcgtgacgct
     4621 cagcaacaac ataggcaggt cattgatgac cagcggcgtc aggtcgcgaa cggccgtcgc
     4681 cccgtccccg tcccggatcg tttccgttac ggcgtgcgcg ccgaacgcat cgaatgccag
     4741 cgtcccctcg acgcgctcga cctgcgcgat gaaatcccgc tcgaaatcga agctctgcaa
     4801 cgcccccagc ccctcgaact gaatcgtcag cgacgtcgac cagtcgtccg tcagttcaac
     4861 atcgaacgga atcgtttcgg cgtcggccgc caggtcctgc cgctccgcgg cggcgggggc
     4921 atgctgcgcc gcgaggcccg gcggctggtt cagccgcacg ctccgcgtgg tccggaaact
     4981 gtcggccccg gcttcgtcgc gaaagtaaat ctccagatcg tagatccgtc gcggcgcccg
     5041 cggcgcaatc tccagattga ccaccgactc cacatcctgc tcgatcgccc cgacgttgat
     5101 cgtctcaagc agctcgccgt cggtgctccg cgcatcgacg taaaccgtca gcggccccgg
     5161 ctccttgtcc cccgcgtgga ccgtcaacgg gacctggacc agcgccggcg tcccgtcgta
     5221 gctcacgacg gccggcgcgg tcggcgcctc gaccgcgtgc ggaatttcgt tcagccggac
     5281 atggtcgatc tccgccacgt cgctcgccgc gctttcgata atgtcccctg cctcggtctc
     5341 atccagcgcc gtaatccgca gcagatactc cgcgtaatcc tcggcgggcg gcgacttcgg
     5401 cagcaggatc tcgcgttcgg tcgccacgtt gcgcggcacg tcctccagcg tctgcgtgtc
     5461 gcgcagcgta tcgtcggccg ggtcgtggat cgcgacccgg acatccagcg tttcggtttc
     5521 gaagtcatcc accgtcaccc gcagcgtcac cggcacggtc gccgtgtcgg tcgagccgtc
     5581 gtagtcgatc tgcttggcgg gcagcacctc cgcgctcagc accgccggcc gctgattgag
     5641 cgtcacgctg aacggctcgc tcaattcgcc cgtgacctcc agggcgtcgg tcgcgcgcag
     5701 ttgcacgccg tacatcggat gaggctgcgc ggggtcatgc gccgtcaggt caagctccag
     5761 cgtgtgagac gtgtcgaccg cccgcgccgg gaagaactga gcgcccgggt cgtcgccgcc
     5821 cggatagtcg atgacggctt caatatcgac cgcggccgaa tccaggtcgg tcgccatcac
     5881 gccggccgcc ggcacggtcg ccgcccacac gtccccatgg ggatagtgaa aaatgaaatc
     5941 atccggccgc gccgtgatcc gggccaggtc gttgatccgc accatgccct cggcggtccc
     6001 gcttgcgccc agacccgccg cggcggattg attcagcgtg tcctcgacca gcagctgcag
     6061 gtcgtacgtc gtcccttcca tcagccccag tgccgtcgcc gtgttggcga tgcccagccc
     6121 ggcctgatcg accgcttcgg ccgccgattc catgacgagc gggaccggct cgcccgaccc
     6181 ctgcggcgcg aacgtcagcg tgtagcgata ctcaggcgtg ttgtcccgat catcggcccg
     6241 cagccgccac cgcagatcgc gcgggtcctt cgcatcctcc gtcgacatct cgatctgcgg
     6301 tgcgggcgcg ccgaacgtat acgtcgtgac cccctcgcca tcgtcggcga gctgcagcgc
     6361 gttgagcgtg acgcgaaacc ggcccgaccc cagcgtggcc agatcagcac gctcctccgc
     6421 tccgacgaac gccggcgggc tttccaagtt atagaccagc cgccggcgat tgctccccaa
     6481 attggtggtg tcgccctggg caaacacgaa ttcgcaggcg ggcgtgccgg gaacgaccgc
     6541 cccctcggcg tcgatctcct caatccgcat cacataggta agcgtggcga gatcgacctg
     6601 ccattcgaac gtcagtgcgg gcgacgtgag ctgcgcgtcg tccaccggcg tgatgaagac
     6661 cggatccgtc tgcgcccgcg ccaccccgcc ggccgccagc accagcccca aaccgacctg
     6721 gcataccacc cgcccgacct gccccaaccc cgccatcgcc caacccccct ctcacccaat
     6781 tcattcgaga ttgcggctct cgtctctcgt cctcgtctct cgaaaaacca tccaacaatc
     6841 gcggccggtg tgggaccggc gccctcgccg gtcctccctt cgagtgcgcg gagcggtagt
     6901 ccggacgaac gcgcagggtg gaggcaaccg cccgacggaa cctgggggac gctatcgcaa
     6961 tacatcctcg tccccgaagg gggcgaacct cagccaaaca tactgcggta ggcgacctgg
     7021 tttcatcctt tcaaagttgc gccgaagacg tggtgaagat ctccctccaa aatcgttctt
     7081 gttctcttcc tcgtcagttc cgccattgcc ccctccggcc ccgacgcctt atggatgcaa
     7141 gcaccagccg aaaccaggaa gcctgagaac gccactctcc tgagtggcct tgccccccat
     7201 cttcaccccg cgctgtgaac ccgccgcttc tcaagaccgc ccctgcgtca agaccgtcgg
     7261 attatgcgag ccggcaagat ggcaggagca tgtaaatcgg caaaatcact gtcatgttaa
     7321 aaatcgcgcc tgcaacatgc atagaaattc gagtttttta tgcatgttgc gcaggaactg
     7381 tatagaagtc accccaattg tagccacatt gggtggcact gatggcaacg ccttcctggt
     7441 atcttttcaa aatcctcggc gccttccgcc tcaaactctt cgtccaccag atagccattt
     7501 cgaacccacc gaaacctcag cggagttcgc cccgacgtaa ctcggcaccc atccggaatt
     7561 ggctcccgtt ccgccgagcc gctcccgctc cgccgcacct gtccccctct gccaagcccc
     7621 cgcccctcac ggcgccggtt tggccagttc cgcttcaatc gcctcgatca gctcgggagc
     7681 gtcgggcgtc accgccgatg cataccgcgc caccagccgc ccgtcccggc caatcaggaa
     7741 cttctcaaaa ttccacttca cctccccggg atgcgggctc ttatctcccg tcagatactc
     7801 aaacagcggc gaaatctcct cgcccttggc gtgcagcttg ccgtacagcg gaaacgtaac
     7861 gtcgtagttg gtctggcaga actccacgat ctccgcctcg gtccccggtt cctggccgcc
     7921 gaaatcgttg catgggaacc ccagaacgcg cagcccctgg tcgaaatagg cgcggtggag
     7981 ctgctccagc cccgcgtact gcggcgtgta gccgcagcgg ctggccacat tgaccagcag
     8041 cagcaccttc ccctgccacg gggcgagcgt ttcggtcgtc gccgggtcgt cgtcggccat
     8101 cggctcgacg ggaacggagt agatcgtgtc ggtcatcgcc ttatcctgtg ccggaaggga
     8161 atcggccgcg cagagcatcc acgccgcggc gcaaagggcg gtcagcgtca atccgtgccg
     8221 ggtcatcatg atctcatcct ttcattgtaa gccggcccgt gcaatgcggc cggccgaaat
     8281 cgcagtccgg gcagaccggg cactccggac agcgcggctt gcgggcggtg cagatccgcc
     8341 ggccgtgcca gatcagcgcg ttcgagaaat gcgtccagtg gctcggcggc agcagctcgg
     8401 ccacggcgaa ctcaatccgg tcggggtcgg tttcggcggt caagcccagc cgcccgatca
     8461 cccgcttgaa atgggtgtcg acgatcagcc cggggtggcc catcgcgcag gcgcgcacca
     8521 ggttggcggt cttgcggccg acgccgggca acgcggtcag ctcctccatc gcctcgggga
     8581 cgcggccgcc gtggcgctcg agcagcgccc ggctgcaatt ctgaatcgag cgtgtcttct
     8641 ggcggaaaaa gccggtggaa tggatcagct tctcgatctc agccgcgtcg gccgccgcga
     8701 acgccgccgg gtcgcgatag cgctggaaca gctccggcgt gacccggttg accgcttcat
     8761 cggtgcactg cgccgagagg attgtcgcga tgaggagctg gaacgggccg tcgaaatgca
     8821 gcgccgtccc cgcgtcgggg taatggcggc gcagtcgggt gctgatccgc cgggcgcgag
     8881 ctttcagttc gggcgccggc gtgcggcgga ggggggacgc ggcgggcttc ggggggcgcg
     8941 gcgacggcac ggggcgatga ttctcgagtt ttaatgcggg tttcaacttg tttaaggctt
     9001 gctaaacaag tttccggact gctaaaaaaa ggtcctgagc ttgactcgcc cgaggattct
     9061 ggcatgacgc cccagcgaaa acaaccccgc agtttcggct cgcgctccgg attcatccat
     9121 acccgcaccc gcaaacagtc cgaactgatg gaggattacg tcgaactgat cgacgacctg
     9181 atcatcgaga agggcgaggc ccgcgccgtc gacgtcgccc gctgtctggg cgtctcgcac
     9241 gtgaccgtct ccaagaccat cggccggctg aaggaagccg ggctggtccg cgcgcaaccc
     9301 taccgctcga tcttcctcac cgagtcgggt cgcgagatgg cccaggccag ccgcc
//
LOCUS       DAOPCO010000154         9241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7069_length_9241_cov_4.702264, whole genome shotgun sequence.
ACCESSION   DAOPCO010000154 DAOPCO010000000
VERSION     DAOPCO010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7069_length_9241_cov_4.702264"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            579..1028
                     /locus_tag="PLS90_11055"
     CDS             579..1028
                     /locus_tag="PLS90_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK02983.1"
                     /translation="MRNLIQRWNQGLRGALGGGVAEKRGARRGFSIIEILVALAILVL
                     AAGGVYHQFFNIDRLGRMRLNEIQGRLLAQQRLEELRALPYARLAEWEPGETATPLGD
                     NLKYAARTTIEPGPGDVLKLTVMVSWRERGAEAVAGNSVTVVGYKGR"
     gene            1025..1798
                     /locus_tag="PLS90_11060"
     CDS             1025..1798
                     /locus_tag="PLS90_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK02984.1"
                     /translation="MNGSLHQSGGRRGMTLVELTVASGALVVVLGLALGIMLESRGAT
                     DEITRRQQAIQYSQQVIEQVTGLLRSAVPPAELTGIRERGVIRPEFRADQLSVPTYEH
                     PLGSYLCMATVSNAAGGEAGESVTRIVRTLDPVMETVAGEVARKDSGRLGLAPLEGMS
                     AAIEFGYAAPRRPGEAVEYQPRWEGEGFPPLVRVRVTVQPRDPKASPIALQTAVAPGM
                     RPPAPAATDTPTTVPAALTGPIAGETDGQDVLPEEEQAP"
     gene            1795..2334
                     /locus_tag="PLS90_11065"
     CDS             1795..2334
                     /locus_tag="PLS90_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02985.1"
                     /translation="MKRRGEHRRARGGYALMAVLGLMALLVVYIATIWSAVGMTIHGS
                     KRYAAHQMEAQGAAAAIEMALAAGPTSGTQTLELAEPLKGRATVDPLDAAHPVWSAMP
                     GLGWRAGDRLVSIVWEGDGAPRQYIANPARQSILAVPAGAAPEPAAAPGPGPGVEPAE
                     AADAAPDRATTITTPTTTR"
     gene            2345..4519
                     /locus_tag="PLS90_11070"
     CDS             2345..4519
                     /locus_tag="PLS90_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HPK02986.1"
                     /translation="MATKTVSPREELAGRFIRLFIAAGLLTMDQVESYTARHKRNKPI
                     LDVLHQEVSLTSFRDLLTAELTFRRVNRVKVNVTAGLTAAGLVTDDELRQLLMVHRPP
                     LDRLIQELREDAVIDEERAGRLLELKGAEGAYERLIGEDAVNAPAVCQWLSRLDSRIM
                     REVALAIALHVFRHNELVNKKKSDALRVELGQGKLDEVTVAIRDELGLDSDRLQEKIA
                     EGLRLPQFSGELDPALRGLLPQSLIRRHLILPCYQDEHSVGIASSDPLNIPLAALIHW
                     VSGRWPQPAFAPARQVIDQINALDGKSAPEGAAGVAVDGQAAPQGKAGTAARRFDADI
                     LVDNTSAVQLVGSLIESAIELRTTDIHLEPARDGLVVRFRIDGDLHRISTIPPGLAQP
                     VISRIKVLADMDVTERRRPQDGHFELNVDSNTFDFRISTLPAILGEKVVIRILDGARL
                     MTGMDDLGLLPQQRERFDWMLDRPHGLILVTGPTGSGKTSTLYTGLQHINTENRNLVT
                     IEDPVEYQLAGINQVQVDPHIGLDFSSGLRSILRQDPDVILVGEIRDGDTARIALRAA
                     MTGHLVLSTLHTNTALGSIDTLIQLGVQPFTLGNSLCGIVSQRLVRRLCAACRQEGRL
                     TDVQALELGLGDDDLNAAVYTAGGCDGCLGSGYYGRTGVFEVIQASEALRPLVAAHRP
                     QSELEAKARELGMLSLAQAGGRKVLAGETSYEEIARRILIDA"
     gene            4599..5819
                     /locus_tag="PLS90_11075"
     CDS             4599..5819
                     /locus_tag="PLS90_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK02987.1"
                     /translation="MAPKQQEPRSEQSPSSLRRALDSRASAEENLNPPTRGGSTAAEI
                     GRVAQDEAEASGAISLLRPSNFDRAVFCRQFAMLIEVGFPVLKALQMLAKRTHHRRLR
                     AAVAETAEAVERGETIHQAMSRNKAIFSPLVVNIIRVGEVGGMLESSLLRLADIMEAK
                     ARIRRKIASASMYPIVALCVALGVITLVLVEAVPRFASIYQESGQTLPKPTQFVINLS
                     NFLVGGWWILLILIVALLLAGRLWTATTAGNRFFSWLALNTPGLSGVNRKIAVARSTR
                     TLGELIAAGFPLTDALLITAQTNENAIVADALRDVHAHVEQGERMTEPLAQARVFPPM
                     VVDMISIGEETGTLDRMLLKIAEIYDAEVETALNGLATIIEPLLIVLMGCVVIFIALA
                     VLLPYFNLVNVVAM"
     gene            6152..7999
                     /locus_tag="PLS90_11080"
     CDS             6152..7999
                     /locus_tag="PLS90_11080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK02988.1"
                     /translation="MPLSKFRTLLLVVLGALMLSGCMERIGRFAADWADKETYPIIAA
                     KQEEVLGEAAPFSIEPAADPLTEHVIEQAGIAGDEFTTAGLTLSLNDALTLALHNNRQ
                     YQARKEQLYLAALRLTGERHVFAPLFSGLITGQRARQPTVETNVEVIGTETVPLPGFA
                     DVSGIPGFEDFEPPTTTIVNTRTETEIGAERFGQLDTSVGVRKLFATGALVTLQLSQQ
                     FMRFYSNSPREQASGLFSASVVQPLLRSAGPDVTLENLRQAERDVLYEVRDFARFERQ
                     FVVERIDEYFRLLQLLDAIRNQQASLETLHYGREQAEELAEAGRLDIFQVNQAQTQEL
                     NAQDALYSVQVAFMQGLEQFRSNLGLPPETAVVPDPAELERLSRQELEPLDIELAAAV
                     EMALARRLDYHNAADQIEDAERFVRVTENALLPVFDLVADFSVTDEEENQPLDLGWKR
                     RRYSYGFNLELPLDRKAERNNYRSALIFLQSARRAEVELHDLIVREVRSLYWELMAAR
                     NSYEIQLRSLEIARMRVESTSLLLQAGRVQVRDVLDADAALLDARNRLTEARINYFVT
                     RLRFLVAIEALEVDMRGLWLEAARSALAAPTPEPLPVESSSAPVALPPP"
     gene            complement(8074..8634)
                     /locus_tag="PLS90_11085"
     CDS             complement(8074..8634)
                     /locus_tag="PLS90_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02989.1"
                     /translation="MTHDRPSAKLVVNDYRTKDDTLMSTAITIHTLEDLLAASQATPE
                     FKDSVRTLAAGPSTLFMADGLPQSRVIFDAASPPVKVLRVVMKLLEEFPNEPFEKVTV
                     DGRSGCSEYVGSLVAEPGDLHVQFTWDCAWRAQQQGWKDAFGFPDQIRAAREFGYQCF
                     LRFDAERGPVSEAFREQESLRDESLA"
BASE COUNT         1478 a         2688 c         3637 g         1438 t
ORIGIN      
        1 gttgttccgc aacgggcatg ggcgttgaag gggtgagggg ctgggggggg aggcgggcgg
       61 aagcccgtcc gggcacccct tgcaggggtt tgggtgtcgg agggggggga cgccagggtt
      121 ccggcctgcg gccgtcacgc tgcgctttat tccgaccgtc cgttgcgcgg acttggccag
      181 caagtcggac gcccgctggc gcggactgtc aaggggcgga ggctcaagcg taatgatcga
      241 gagccgagga cgagggacga gagccgggag ccgaattctg ggagggccga tgggggtgat
      301 gatctcacgc cgagacgcag agacagaggc gcaggcgcgg ggaggttgaa cctgggacgg
      361 acgggatggg cttgaagcgg gggttcgccg agggatgcaa aaaggagggg cgatgccact
      421 ccggggagtg gcgctcccag ggcggtccac aggaacggtc cggatcgcgg ggcgggcaat
      481 catcacttga cattgaaccg ccggggcttt gaataggggg agtgtcgtag gaattttcgg
      541 ttgtcttcat catcctcgcg ggaggggttt gacgcatctt gaggaacctg atccaacgat
      601 ggaatcaggg gctgcgcggc gccttgggcg gcggggtcgc ggagaagcgc ggcgcgcggc
      661 gggggttttc gatcatcgag atcctggtcg cgctggcgat cctggtgctg gccgccgggg
      721 gggtctacca tcagtttttc aacatcgatc gcctggggcg gatgcggctg aatgaaatac
      781 aggggcggct gctcgcgcaa cagcggctgg aagagctgcg cgcgctgcct tacgcgcggt
      841 tggccgagtg ggaaccgggg gagacggcca cgccgctggg cgacaatctg aaatacgccg
      901 cgcggacgac gatcgagccg ggacccggcg acgtgctgaa gctgacggtg atggtgagtt
      961 ggcgcgaacg cggggccgag gcggtggcgg gcaacagcgt aacggtcgtg gggtacaagg
     1021 ggcgatgaac gggagcctgc accagtccgg cggtcgaagg ggcatgacgc tggtggagct
     1081 gacggttgcg tcgggtgcgc tggtcgtcgt gctggggctg gcgctgggga tcatgctgga
     1141 atcgcgcggg gcgaccgacg agatcacgcg gcggcagcag gcgatccagt acagccagca
     1201 ggtgatcgag caggtgacgg ggctgctgcg gtcggcggtc ccgccggccg aactgacggg
     1261 gatccgggaa cggggggtga tccgccctga attccgggcc gatcagctca gcgtgccgac
     1321 ctacgagcat ccgctcggat cgtatctctg catggcgacg gtgagcaacg ccgccggggg
     1381 cgaggcgggg gagtccgtga cgcggatcgt gcggacgctg gacccggtga tggagaccgt
     1441 cgcgggggaa gtggcgcgca aggatagcgg ccggctgggt ctggcgccgc tggaggggat
     1501 gagcgcggcg attgaattcg gatatgccgc gccgaggcgg ccgggcgagg cggtcgagta
     1561 tcagccgcgg tgggagggcg aagggtttcc gccgctggtg cgggtgcggg tgacggtgca
     1621 gccgcgcgac ccgaaggcga gcccgatcgc gctgcagacg gcggtggcgc cggggatgcg
     1681 gccgccggcg ccggcggcga ccgacacgcc gacgaccgtc ccggcggcgc tgaccgggcc
     1741 gatcgccggc gagaccgacg gccaggacgt gctgccggaa gaggagcagg cgccatgaag
     1801 cgcaggggcg agcatcggcg ggcgcgcggg ggctatgcgt tgatggccgt gctggggctg
     1861 atggccctgc tggtggtgta catcgcgacg atctggagcg cggtcgggat gacgattcac
     1921 gggagcaaac gctacgccgc ccatcagatg gaggcgcagg gggcggcggc ggcgattgag
     1981 atggcgctgg ccgcggggcc gacgagcggg acgcagacgc tggagcttgc cgagccgctg
     2041 aaggggcggg cgaccgtcga tccgctcgat gccgcccatc cggtgtggag cgcgatgccg
     2101 gggctggggt ggcgcgcggg cgaccggctg gtgtcgatcg tctgggaagg ggacggggcg
     2161 ccgcggcaat acattgccaa tccggcgcgc cagagcattc tggccgtgcc ggcgggcgcg
     2221 gcgcccgaac cggcggcggc gccggggccg gggccggggg tggagccggc cgaagcggcg
     2281 gacgccgcac cggatcgcgc cacgacgatc accaccccga cgacgacacg ctgaggcaga
     2341 cccgatggcg accaagaccg tcagcccgcg cgaagagcta gccggccgat tcatccggct
     2401 gttcattgcg gccgggctgc tcacgatgga ccaggtggag tcgtacacgg cccgtcacaa
     2461 gcgcaacaag ccgattctgg acgtcctgca tcaggaagtc tcgctgacgt cgttccgcga
     2521 cctgctgacg gcggagctga cgttccggcg cgtgaaccgc gtcaaggtga acgtgacggc
     2581 ggggctgacc gccgccggcc tggtgaccga cgacgaactg cgccagttgc tgatggtgca
     2641 ccgcccgccg ctggaccggc tgatccagga gctgcgcgag gacgccgtga tcgacgagga
     2701 aagggcgggg cggctgctgg agctgaaggg ggcggaggga gcgtatgagc ggctgatcgg
     2761 ggaggacgcg gtgaatgcgc cggccgtgtg ccagtggctg agccggctgg attcgcggat
     2821 catgcgcgaa gtggcgctgg cgatcgcgct gcacgtcttc cggcacaacg agctggtgaa
     2881 caagaagaaa tcggacgcgc tgcgggtgga gctcggccag gggaagctcg acgaagtgac
     2941 ggtggcgatc cgggacgagc tggggctgga cagcgaccgg ttgcaggaga agatcgccga
     3001 ggggctgcgg ctgccgcagt tcagcggcga gctcgacccc gcgctgcggg ggctgctgcc
     3061 gcagagcctg atccggcggc acctgatcct gccgtgctac caggacgagc acagcgtggg
     3121 gatcgcgtcg tccgatccgc tgaacatccc gctggcggcg ttgatccact gggtgtcggg
     3181 gcggtggccg cagccggcgt ttgcccccgc gcggcaggtg atcgaccaga tcaacgcgct
     3241 ggatgggaag agcgcgccgg agggcgcggc gggggtcgcc gtcgacggtc aggcggctcc
     3301 gcaagggaaa gcgggcacgg cggcgcggcg gttcgacgcc gacatcctcg tggacaacac
     3361 ctcggcggtg cagctcgtcg gctcgctgat cgagagtgcg atcgagctgc ggacgacgga
     3421 cattcacctc gagccggccc gcgacgggct ggtggtgcgg ttccggatcg acggcgacct
     3481 gcatcggatc agcacgattc cgccggggct ggcccagccg gtgatttcgc ggatcaaggt
     3541 gctagccgac atggacgtga ccgagcggcg gcggccgcag gacggccatt tcgagctgaa
     3601 cgtggacagc aatacgttcg acttccggat ttcgacgctg ccagcgatcc tgggggagaa
     3661 ggtcgtgatc cggatcctgg acggcgcgcg gctgatgacc gggatggacg acctgggtct
     3721 gctgccgcag cagcgggaac ggttcgactg gatgcttgat cggccccatg ggctgatcct
     3781 cgtgacgggg ccgaccggca gcgggaagac ctcgacgctc tacacgggcc tgcagcacat
     3841 caacaccgag aaccgcaacc tggtgacgat cgaggacccg gtcgaatacc agctcgcggg
     3901 gatcaaccag gtgcaggtgg acccgcatat cgggctggat ttttcgagcg gtctgcgctc
     3961 gatcctgcgg caggatcccg acgtgatcct ggtgggcgag atccgggacg gcgacacggc
     4021 gcggatcgcg ctgcgggcgg cgatgacggg gcacctggtg ctgtcgacgc tgcacaccaa
     4081 cacggcgctg gggtcgatcg acacgctgat ccagctcggc gtgcagccgt tcacgctggg
     4141 caattcgctg tgcgggatcg tgagccagcg gctggtgcgg cggctgtgcg cggcgtgccg
     4201 gcaggaaggg cggctgaccg acgtgcaggc gctggagctg gggctgggcg acgacgatct
     4261 gaacgcggcg gtgtacacgg ccggcggatg cgacggctgc ctcggctcgg gctattacgg
     4321 gcgcacgggg gtgtttgagg tgattcaggc gagcgaagcg ctgcggccgc tggtcgcggc
     4381 gcaccggccg cagagcgaac tggaggccaa ggcgcgcgag ctggggatgt tgagcctggc
     4441 ccaggcgggc gggcgcaagg tgctggccgg cgagacgtcg tatgaggaaa ttgcgcggcg
     4501 gatcctgatc gacgcctgac gcccggcgcg cggcggaacc ccctatgcgt tggcccgccc
     4561 ggcgcgcggc ctgacgccga aaccacggag gacccgacat ggcacccaag caacaggaac
     4621 cgcggagcga gcagagcccg agcagcctgc ggcgcgcgct ggacagccgg gcgtcggccg
     4681 aggagaacct caacccgccg acgcgggggg gctcgacggc ggccgagatc ggccgggtgg
     4741 cgcaggatga ggcggaggcg agcggggcga tttcgctgct gcggccgtcc aattttgatc
     4801 gcgcggtgtt ctgccggcag ttcgcgatgc tgatcgaggt gggctttccg gtgttgaagg
     4861 cgctgcagat gctggccaag cggacgcatc accggcggct gcgggcggcg gtggcggaga
     4921 cggccgaggc ggtcgagcgg ggcgagacga tccatcaggc gatgagccgc aacaaggcga
     4981 tcttttcgcc gctggtggtc aacatcatcc gggtgggcga ggtcggcggc atgctggaga
     5041 gctcgctgct gcggctggcc gacatcatgg aagcgaaggc gcggatccgg cgcaagatcg
     5101 cctcggcgtc gatgtatccg atcgtggcgc tgtgcgtggc gctgggggtg atcacgctgg
     5161 tgctggtgga ggcggtgccg cggtttgcct cgatttatca ggaatcgggg cagacgctgc
     5221 cgaagccgac gcagttcgtg atcaaccttt ccaatttcct ggtggggggc tggtggatcc
     5281 tgctgatcct gatcgtggcg ctgctgctgg cggggcggtt gtggacggcg acgacggcgg
     5341 gcaaccggtt cttctcgtgg ttggcgctca acacgccggg gctgagcggg gtcaaccgca
     5401 agatcgcggt ggcgcgctcg acgcggacgc tgggagagct gatcgcggcg gggtttccgc
     5461 tgaccgacgc gctgctgatc acggcgcaga cgaacgagaa cgcgatcgtc gccgacgcgc
     5521 tgcgcgacgt ccacgcgcat gtggagcagg gggaacggat gaccgagccg ctggcgcagg
     5581 cgcgggtttt tccgccgatg gtggtcgaca tgatctcgat cggggaagag accgggacgc
     5641 tggaccggat gctgctgaag atcgcagaga tttacgatgc ggaagtggaa acggcgctga
     5701 acgggctggc gacgatcatc gagccgctgc tgatcgtgct gatggggtgc gtcgtgattt
     5761 tcatcgcgct ggcggtcctg ctgccgtatt tcaatctggt gaacgtggtg gcgatgtaag
     5821 gggcgcggcg ggaatctccc gtcacccctt tcaggggctt gggggattgg gggtgattat
     5881 ctcacgccgt tggcgccgag acgcggagag gtgcaggcgc ggaggttgag tctgcgacgg
     5941 atcggatggg cttgaatcgg ggggtcaccg tgagggatga agttagagga caaggccgct
     6001 caggagagcg gcgttcccag gccggttgct tctttccgtg aggcgtattt ctttttttcc
     6061 gcacaaccct ttcgtgcctt tcgcttctgg aagtgtagat taagaggcaa tccccggccc
     6121 ggggagaccc agcttgccgt ccatccggtc catgccgcta tcgaagttcc gcactctcct
     6181 gctggtcgtg cttggggcgc tgatgctgtc gggctgcatg gagcggatcg ggcggttcgc
     6241 cgccgactgg gccgacaagg aaacgtatcc gatcatcgcg gccaagcagg aagaggtgct
     6301 gggggaggcg gcgccgttca gcatcgaacc ggccgccgac ccgctgacgg agcatgtgat
     6361 cgagcaggcg ggcattgccg gcgatgagtt cacgacggcg gggctgacgc tgtcgctcaa
     6421 cgacgcgctc acgctggcgc tgcacaacaa ccgccagtat caggcgcgca aggaacagct
     6481 ctacctggcg gcgctgcggc tgacgggcga gcgccacgtg ttcgcgccgc tgttcagcgg
     6541 gctgatcacg gggcagcgag cccgccagcc gacggtcgaa acgaatgtgg aggtcatcgg
     6601 gaccgagacg gtccccctgc ccggttttgc ggacgtgagc gggatcccgg gcttcgagga
     6661 cttcgagccg ccgaccacga cgatcgtcaa cacgcggacg gagacggaga tcggggcgga
     6721 gcggttcggg cagcttgata cgagcgtcgg ggtgcggaaa ctgttcgcca cgggcgcact
     6781 cgtcacgctg cagctcagcc agcaattcat gcggttctac agcaattcgc cgcgcgagca
     6841 ggcgagcggg ctgttcagcg cgtcggttgt ccagccgctg ctgcggtcgg ccgggccgga
     6901 cgtgacgctg gagaacctgc gccaggccga acgcgacgtg ctgtatgaag tgcgcgactt
     6961 cgcgcgtttc gagcgccagt tcgtcgtcga gcggatcgac gaatatttcc ggctgttgca
     7021 gttgctcgat gcgatccgca accagcaggc gtcgctcgag acgctgcact atgggcgcga
     7081 gcaggccgag gaactggccg aagccgggcg gctcgatatt tttcaggtca atcaggcgca
     7141 gacgcaggaa ctcaacgcgc aggacgcgct ctactcggtt caggtggcgt tcatgcaggg
     7201 gctggagcaa ttccgttcca acctggggct gccccccgag accgccgtcg ttccggatcc
     7261 ggccgaactg gagcggctct cgcggcagga gctcgagccg ctcgatatcg agctggccgc
     7321 ggccgtggag atggcgctgg cccgacggct ggattaccac aacgccgccg atcagatcga
     7381 ggacgccgag cggttcgtcc gggtcacgga aaacgcgctg ctgcccgtgt tcgacctggt
     7441 ggccgacttc agcgtgaccg acgaggagga gaaccagccg ctggatctgg ggtggaagcg
     7501 ccggcgctac agctatgggt tcaatctgga actgccgctt gatcgcaagg cggaacggaa
     7561 caactaccgg agcgcgctga tttttctgca gtcggcgcgg cgagccgagg tcgagctgca
     7621 cgacctgatc gtgcgcgagg tgcgttcgct gtattgggag ctgatggcgg cgcgcaacag
     7681 ctacgagatc cagttgcgca gcctggagat cgcgcggatg cgcgtggaga gcaccagcct
     7741 gctgctgcag gccgggcggg tgcaggtgcg cgacgtgctg gacgcggacg ccgcgctgct
     7801 tgacgcgcgc aaccggctga cggaggcgcg gatcaactat ttcgtcacgc ggctgcggtt
     7861 tctggtggcg atcgaagcgc tggaagtcga catgcggggg ctgtggctgg aggcagcgcg
     7921 gtcggcgctg gcagcgccca cgcccgaacc gttgccggtg gagtcgtcat cggcgccggt
     7981 ggcgctgccg ccgccgtgag gccattgcga gatatgtcgg cgggggcgag gcggaaccgg
     8041 cggggggcgg ggcgggtgtc cgcgctggtc agctcacgcc agggattcgt cgcgaaggct
     8101 ctcctgctcg cgaaaggcct cggagaccgg tccgcgctcg gcgtcgaagc gcaggaagca
     8161 ctggtagccg aattcgcgcg ccgcgcggat ctgatcgggg aatccgaagg cgtccttcca
     8221 gccctgctgc tgggcgcgcc aggcgcaatc ccaggtgaac tggacgtgca ggtcgcccgg
     8281 ctcggcgacg agcgagccga cgtattcgga gcagccggag cggccgtcca cggtgacttt
     8341 ctcgaagggt tcgttgggaa actcttcgag cagtttcatc accacgcgca ggaccttgac
     8401 cggcggggag gcggcgtcga aaatcacgcg gctctgcggg aggccgtcgg ccatgaacag
     8461 ggtggatggg ccggcggcga gcgtgcggac ggagtccttg aattcgggcg tcgcctggct
     8521 tgccgccaag aggtcctcca gggtgtgaat cgtgatggcg gtgctcatga gcgtgtcgtc
     8581 cttcgtgcgg tagtcgttca ccaccagttt agcagagggg cggtcgtggg tcaaacgggg
     8641 accccagaaa caagccgtgg attcgccccc ttcgggacgg gaatggttgg ggatggtcgt
     8701 ccccagattc cgctacgctt caccggggcc taatggaaag ggcgcccgtc cggggcgacg
     8761 tgtggcatcc ttgacaggat gcggggggcc agatgccggc cgctccggcc accccttcgg
     8821 ggtttgattc gttgggggcg gcgacgcagg gttccggcct gcggccgtca ccccaagatg
     8881 cggctcactt ctggcatccc tccgggatgc gggggaatag gttggcggat gaccggtggt
     8941 atcgctgcgc tcaaccaccg gctacgatcc ggcatccctt cgggatgcgg ggggccagat
     9001 gccggccgct ccggccaccc cttcggggtt tgattcgttg ggggcggcga cgcagggttc
     9061 cggcccgcgg ccgtcaccct gcgctttatt ccagtcgtcc gctccgcgga cttgtccggg
     9121 acgaccagcc ggaaccgccg gtgcggtcgc acggcggggg gggcgcgaag atcaagatgc
     9181 ggctcgcttc tggcatccct ccgggatgcg ggggaatagg ttggcggatg accggtggta
     9241 t
//
LOCUS       DAOPCO010000155         9205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7099_length_9205_cov_4.482623, whole genome shotgun sequence.
ACCESSION   DAOPCO010000155 DAOPCO010000000
VERSION     DAOPCO010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_9205_cov_4.482623"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLS90_11090"
     CDS             <1..222
                     /locus_tag="PLS90_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02990.1"
                     /translation="EPGRIELEALEPGQSILVVAGPVRGVGADGAALPAQGPPRSRLA
                     VEPAGYHYEAETGRLFVLHPAGRIEIYLE"
     gene            229..2484
                     /locus_tag="PLS90_11095"
     CDS             229..2484
                     /locus_tag="PLS90_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02991.1"
                     /translation="MLPRITIVLIVLAWANPAGAFVGFPGWREGTEHAVENRGLTLTL
                     GEDSGRILAVDNRLTTESYELLEPLPLRLVVDGAPLGEEFELTARYLDADPTFVYHAG
                     DLATSVTWRVRPDKDFVEKEISITNHGTCPVMLDEVTVFHFRLPSNAMMVHPHHDPSD
                     AAWLINLFLRGERGGLYAGVENPFVELVRPTFWEFEFTYRPGWRLEPGATFVSEPGFL
                     GTYAMQGIYAFKELHKLANMVRREIGRDYNNEARPLTMRIDQEVLDWGEVWAMQDFMQ
                     TIMPPQTFKKPGYYLRAIGPVDDVEESKTFAEMIAGIGHIAHIEWNTWFQLDDPGSPA
                     IWIDSSGGELRLRPNEGWSEMVRHALEQGVYSGTMEGQPHPGYHARADWLRRARDGSY
                     LHQSRALNGEFTRMSVCLANPAFFDWYAEQYDGLIREYDLYMLAWDDSGGYLRTKYYS
                     GYECWAENHGHPPGDAAFYVWRNWMGFFRRMHELHPQLAMRVASGLQRGYPWILKDLI
                     EHHTDFYDYGPGASWWRSVNYRFIPPYKCTSTMAAGTWDELHYNFFRSFSIAPQAMIW
                     GVDVRYGWPGIPQTPEQHAFFRKWMAWADENIEYLRVRRDLFREPWGDKRLDAVHVDM
                     EANFPWDDPQLHGTAHCIGDRGFLFIFNPAEMVRVAEIPLSDWIGLEAGAAYDLREIY
                     PGEAVALGRYDRGASARIAVPADTVMLIEISPAGAGAAPGAPNLPADAPVDRAFYGWS
                     EIPWGEIMAQP"
     gene            2594..3643
                     /locus_tag="PLS90_11100"
     CDS             2594..3643
                     /locus_tag="PLS90_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK02992.1"
                     /translation="MARVKDHRRIGWGVIGCGGIADKRTIPGMKLSARTRLIGVADRY
                     PEVAKRVAQAQQCEAFASVEAMLRNPDIEAVYIASPVFLHKTHAMQAADAGKQILLEK
                     PLGVTLAECREVAAYCRRKKVRLMPALMMRFHPLHRQLRQWVAGGQLGRIVQARAQLS
                     HFHVEYNADGSRNWRQDPKKSGGGALMDMGIHCVDLLRVITGREIVNVSARIETLEND
                     YKIEDTGVLLFSFDNGAVGIVDSTFAARGAQHVIELYGTERSVFVENSISQIEEGTWY
                     EATGPIKRKLPYRKRPNYQYAMYTSEVDEMNRAIMEDDAPAITPRDALIAQAVVQTGY
                     QAAKAGRRLAIPSFR"
     gene            3936..5060
                     /locus_tag="PLS90_11105"
     CDS             3936..5060
                     /locus_tag="PLS90_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK02993.1"
                     /translation="MAGKESNRLSTPPGRITLHDVAREAEVSAATVSKVLSGAKGAIR
                     ISKQTRERVMAVARRMNYIPNASAQALRRGSLSAIGLYFPFGLHPWVSSAYSQLTRHI
                     SILLEEERHSLVIMINPPPRKKAEPARFPSFLYNQGVDGVICVHQVPEALLEELRQRK
                     MPYVLLNCDTGYPENCVDADERWVMQQSLQYVYDLGHRRIIFAGRSTSHPSYDIKREV
                     YVEFMKRKGLPALPDVYEITDPLSDERGDFGEKIAERILRQAERPTAIVAYNDITALA
                     LIHAFRDRGISIPEEISIVGCDDIHESLVARPRLTTFRFPADRLAKAAVEMVLKQVRT
                     HEPQKSIAVKHEFLARDSCVQLAESRSHRAKRPASAGAFK"
     gene            5447..7621
                     /locus_tag="PLS90_11110"
     CDS             5447..7621
                     /locus_tag="PLS90_11110"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK02994.1"
                     /translation="MAQPGHDVALVLDLGEFESAAAALAYVEDVDWSNPQSSEAVTCT
                     HALAATELRDYLCRLTGADADDERNFPIRDDDGDLAGPAILIGGPATNRQTAALAAER
                     GGDLGIPAEEQGFRILGLPEHEPPVLALAGADRVGTLYAAYELLEELGVRWYGPGEVN
                     EEAPRREGFAIPALDRVEAPGFRSRGFWAWEDRATPEFIEWMGRNRMNFWTVEDQQRA
                     QLKLRGIRLTCGSHHTQWRFIHPDHPYPYDVARFTGDENKPADPYREGEYQGDVNGDG
                     ALSYSEVHPEWYGLQKGKRSFRFEYDFGDNICDSNEDGVTEFYRNLVQDLIDGQWRWA
                     DSINFWMFDSGVWCECEACQALGTPTDRNLRLVHRLRQAIDAARAAGRLQRPVQIEFL
                     AYFDVIEPPTRPLPEDFDYDGCVATFFPIRRCYVHAFDDPACTELNAPLHAQYTGWAT
                     DPERHYRGQIFIGEYYNVSFFRNLPLVLDQILASDIPYFHRTGARHMHYMHAPGGMLG
                     PRALTNWLLARMLWEPEADAGALLEDYFTGRYGAVAPTMRRLYDHLRTGLSNTTLLKS
                     VMARRLNEQRRDLFPDRHLKYEETHPETDDGPDFVEILKEMDAAGRLMGEALRTPAPR
                     RVRLRLMEDQRMVRYADHTVRLYDRLLATQRALAAGDRDAAEAAYAEATIHADRLRVD
                     ILSTSMASSHANDKNGLEASLVSGVYEEIGKAFKTGGEAAAR"
     gene            complement(7703..8902)
                     /locus_tag="PLS90_11115"
     CDS             complement(7703..8902)
                     /locus_tag="PLS90_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK02995.1"
                     /translation="MRCLKHDRRLSRGLMAACLLAGLAAAAHAWEGFDHETWREIAQP
                     EPPRLQTPQAGRLDLAPLLLADDGTTISTADQWEEKRQRLRATLQAVIGEPTNLEPVA
                     PDAYEVDRHADPDFERIHLMINADSDGGRIPAYLFLPLPAAQAPLPAVIVLHQTVAQG
                     KEEPAGVNMSFDLNFAEDLARRGLITIAYDAPGFGERIPPGTDPYHNSHEFFRRHPDW
                     SVLGRMNWDFSRIVDFLQTRPEVDPERIAVIGHSHGAYGGVMGAVFDPRVCAVVASCG
                     INTFRADPTPWRWSHATPLLPRLGFFTDDIRTAPFDWHEVLACLAPRPFFYFGTLADD
                     IFPNTDGLRDVFEQVRGVYALYDAPALLRLYLVDGPHSFPPDRQNLAYDWLERTLGVK
                     GEPAHSP"
     gene            complement(8889..>9205)
                     /locus_tag="PLS90_11120"
     CDS             complement(8889..>9205)
                     /locus_tag="PLS90_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK02996.1"
                     /translation="GVGSWLRPDPAAQRARTDAFFTRLTEPEPAAPPPAATGPADFSP
                     LPFIALVTAALGALLLLVVLLFVSLEQGLPSLFVGAGMLLLGLVLWMLDRRKEKNRHA
                     LP"
BASE COUNT         1575 a         2660 c         3477 g         1493 t
ORIGIN      
        1 gagccgggga ggattgagct ggaggcgctg gaaccggggc aatcgatcct ggtggtggcg
       61 ggtccggtgc gaggggtggg cgcggacggc gcggcgttgc cggcgcaggg gccgccgcgg
      121 tcgcggctgg ccgtcgaacc ggcgggttat cattatgagg cggagaccgg tcggctgttt
      181 gtgctgcacc cggccggccg gattgaaatc tatctggagt agcggagcat gttgccacga
      241 atcacgatcg tgctgattgt gctggcctgg gcgaatccgg ccggcgcctt cgtcggattc
      301 cccggctggc gggaggggac cgagcacgcg gtggagaacc gcgggctgac gttgacgctg
      361 ggcgaggaca gcgggcgcat cctcgcggtc gacaatcggc tgacgaccga aagctacgag
      421 ctgctcgagc cgctgccgtt gcggctggtg gtggacggcg cgccgctggg cgaggagttc
      481 gagctgacgg cgcgctacct cgacgccgat ccgaccttcg tttatcacgc aggggatctg
      541 gcgacgagcg ttacgtggcg ggtgcggcca gacaaggact tcgtggagaa ggagatcagc
      601 atcacgaacc acgggacgtg cccggtgatg ctggacgaag tgacggtgtt ccatttccgg
      661 cttccgtcta atgcgatgat ggttcatccg caccacgacc cgagcgacgc ggcctggctg
      721 atcaacctgt tcctgcgcgg ggagcggggg gggctttacg cgggggtgga gaatccgttc
      781 gtggaactgg tgcggccgac gttctgggaa ttcgagttca cgtaccggcc ggggtggcgg
      841 ctggagccgg gggcgacgtt cgtgagcgag ccggggttcc tgggaacgta tgccatgcag
      901 gggatctacg ccttcaagga actgcacaag ctggccaaca tggtgcgccg ggaaatcggc
      961 cgcgattaca acaatgaggc gcggccgctg acgatgcgga tcgaccagga ggtgctcgac
     1021 tggggcgagg tgtgggcgat gcaggatttc atgcagacga tcatgccgcc gcagacgttc
     1081 aagaagccgg gctactacct gcgcgcgatc gggccggtgg acgacgtgga ggagagcaag
     1141 acgttcgccg agatgatcgc cgggatcggg cacatcgcgc atatcgaatg gaacacgtgg
     1201 ttccagctcg acgatccggg cagcccggcg atctggatcg acagctcggg gggggagctg
     1261 cggctgcgac ccaacgaggg gtggtccgag atggtgcgcc atgccctgga gcagggggtc
     1321 tattccggga cgatggaggg gcagccccac cccggctacc acgcgcgggc cgactggctg
     1381 cggcgggcgc gcgacggctc gtatctgcac cagagccggg cattgaacgg cgaattcacc
     1441 cggatgagcg tctgcctggc caatccggcg ttcttcgact ggtatgctga gcagtatgac
     1501 gggctgatcc gggagtatga cctctacatg ctggcgtggg acgactcggg cgggtatctg
     1561 cgaacgaaat actactcggg ctacgaatgc tgggcggaga accacgggca tccgccgggc
     1621 gacgcggcgt tctacgtctg gcgcaactgg atggggtttt tccggcggat gcacgagctg
     1681 cacccgcagt tggcgatgcg cgtcgcctcc ggcttgcagc ggggctatcc gtggatcctg
     1741 aaggatctga tcgagcacca cacggacttt tacgattacg gcccgggggc gtcgtggtgg
     1801 cggtcggtca actatcggtt cattccgccc tacaagtgca cgtcgacgat ggcggcgggg
     1861 acgtgggacg aactgcacta caatttcttc cggtcgttct cgatcgcgcc gcaggcgatg
     1921 atctgggggg tggacgtgcg ctacggctgg ccgggcatcc cgcagacgcc cgagcagcat
     1981 gcgtttttcc gcaagtggat ggcgtgggcg gacgagaaca tcgaatacct gcgcgtgcgg
     2041 cgggacctgt tccgcgagcc gtggggggac aagcggctgg acgccgtgca tgtcgacatg
     2101 gaagcaaact ttccgtggga cgatccgcaa ctgcacggga cggcgcactg catcggcgac
     2161 cgcggcttcc tgttcatctt caatccggcc gagatggtcc gcgtcgcgga gatcccgctg
     2221 agcgattgga tcgggctgga ggccggcgcg gcgtatgacc tgcgcgagat ctaccccggc
     2281 gaggcggtgg cgctgggccg gtatgaccgg ggggcgagcg cgcgaattgc cgtgccggcc
     2341 gacacggtga tgctgatcga gatcagtccg gcgggggcgg gggcggcgcc cggggcgccg
     2401 aacttgccgg cggatgcgcc ggtcgatcgg gcattttatg gctggtcgga aataccctgg
     2461 ggtgagatta tggcgcaacc gtgaaacagg tcgccgcgcc gcctgccgca tcgctccccg
     2521 atccggcgga gcgacgcagt cagccgcgcg ggcggcgaag ccggcaagac caagcgcagg
     2581 gaggaaagtg gagatggctc gcgtgaagga tcatcgcagg atcggctggg gggtgatcgg
     2641 ctgcggcggg atcgcggaca agcggacgat cccgggaatg aagctgtcgg cgcggacccg
     2701 gctgatcggg gtggccgacc ggtatccgga agtcgccaag cgggtggccc aggcgcaaca
     2761 gtgcgaggcg ttcgcgtcgg tcgaggcgat gctccggaat ccggatatcg aggcggtcta
     2821 cattgcgtcg ccggtcttcc tgcacaagac acacgcgatg caggcggccg acgccggcaa
     2881 gcagatcctg ctggagaagc cgctgggggt cacgcttgcg gaatgccgtg aggtcgcggc
     2941 ctactgccgg cggaagaagg tgcggctgat gccggcgctg atgatgcggt tccatccgct
     3001 gcatcgccaa ctgcgccagt gggtggcggg gggccagttg gggcggatcg tgcaggcgcg
     3061 ggcgcagctc tcgcattttc acgtggagta taatgccgac gggtcgcgca actggcgcca
     3121 ggacccgaag aaatccgggg gcggggcgct gatggacatg ggcatccact gcgtcgacct
     3181 gctgcgggtg atcacggggc gcgagatcgt caacgtgtcg gcgcggatcg agacgctgga
     3241 gaacgattac aagatcgagg ataccggcgt gctgctgttt tcgttcgaca acggggcggt
     3301 ggggatcgtc gattcgacgt tcgcggcgcg cggggcgcag catgtcatcg agctttacgg
     3361 gacggaacgg agcgtgttcg tcgagaactc gatcagccag atcgaggagg ggacgtggta
     3421 tgaggcgacc ggcccgatca agcgcaagct gccgtaccgc aaacggccga actaccagta
     3481 tgcgatgtac accagcgagg tggacgagat gaaccgggcg atcatggagg acgacgcgcc
     3541 ggcgatcact ccgcgcgacg cgctgattgc ccaggcggtg gtgcagacgg ggtatcaggc
     3601 ggccaaggcg ggtcgccggc tggcgattcc gtcgttccgg tgagggggag ggaaacaaag
     3661 gcgggttttt tcgagagccg aggacgaggg acgggagccg agagctgaat ttccgggggg
     3721 ggcgttggga gcgattacgc ggacgaggga cgaaggacga ctttgggggg gaggcgaggg
     3781 ggcggagccc cgggttttta gttgaagggt cggttgcctc aatgcgattc ttggatggta
     3841 gctttgtcat tctccgcccg ggcgtctgaa gcgggaattc agggattttt gcggagaatt
     3901 atccggcccc catctggcaa caggatcgac tgcgcatggc aggtaaagag tccaaccgac
     3961 tatcgacccc tcctggccgc atcacccttc acgacgtggc gcgggaagcc gaggtctcgg
     4021 cggcgacggt gtccaaagtg ctctcgggcg ccaagggggc gatccgcatc agcaagcaga
     4081 cgcgcgagcg ggtgatggca gtcgcccggc ggatgaacta catccccaac gcgtcggcgc
     4141 aggcgttgcg ccgagggtcg ctgagcgcca tcgggctgta cttcccgttc ggtctgcatc
     4201 cgtgggtcag cagcgcctat tcgcagttga cgcgccacat cagcatcctg ctggaggagg
     4261 aacgccacag tctggtgatc atgatcaacc cgccgccgcg caagaaggcc gagccggcgc
     4321 gcttcccgtc gttcctgtat aaccaggggg tcgacggcgt gatctgcgtg caccaggtgc
     4381 cggaggcgtt gctggaggag ttgcgccagc gcaagatgcc gtatgtcctg ctcaattgcg
     4441 acacgggtta cccggagaac tgcgttgatg cggacgagcg ctgggtgatg cagcaatcgc
     4501 tgcagtacgt ttacgacctg gggcaccggc ggatcatctt cgcggggcgg tcgacgagcc
     4561 atccgagcta cgacatcaag cgggaagtgt atgtcgagtt catgaagcgc aagggtctgc
     4621 cggcgctgcc cgacgtgtat gagatcaccg atccgctgag cgatgagcgg ggggatttcg
     4681 gggagaaaat cgccgagcgg atcctgcggc aggccgagcg gccgacggcg atcgtggcct
     4741 acaacgacat cacggcgctg gcgctgattc acgccttccg agaccggggg atcagcattc
     4801 cggaagagat cagcatcgtc ggctgcgacg acatccatga gtcgctggtg gcgcggccgc
     4861 ggctgacgac gttccgcttc cccgccgacc ggttggccaa ggcggcggtg gagatggtgc
     4921 tgaaacaggt gcggacgcac gaaccgcaga agtcgattgc ggtgaagcac gaatttctgg
     4981 cgcgcgattc gtgcgttcag ttggcggaga gccggtcgca ccgcgcgaag cgtccggcgt
     5041 cggcgggggc gttcaagtaa gggccgcgat ccacagcggt gtcgacgcgt tgcgttgcaa
     5101 cactctgctc ccgcactcca ggaaggtgac tcgtagtcgc cggcatcagg caatccgatc
     5161 agtttcaagt gaattggaaa tctgtcggat cggacggatc cgaccgatgg ccggattgta
     5221 ccagcgtcgc gccggacggg aacgggcggg cttgacacgg cggcggcgac atgggaaaac
     5281 gattgctcat cccgggataa cgtttgctca gacgtcgccg gcccgaccaa ttccccttcc
     5341 ctgaccggcc ccgggttccc cttcaggagc gcgccccatg cttcgttcgt tgcaccgtct
     5401 tgctttacca ttggttatcc tcctcgcgat cgggagcgca gcggtcttgg cccaaccggg
     5461 ccacgacgtt gcgctggtgc tcgacctggg cgagttcgag agcgccgcgg cggcgctggc
     5521 ctatgtcgag gacgtcgatt ggagcaaccc ccagtccagc gaagcggtga cctgcacgca
     5581 cgcgctcgcc gcgaccgaac tgcgcgacta cctttgccgg ctgacggggg ccgatgctga
     5641 cgacgaacgg aattttccga ttcgcgacga cgatggcgac ctggccgggc cggcgatcct
     5701 gatcgggggg ccggccacga accggcagac ggcggcgctc gccgccgaac ggggcggcga
     5761 tctgggcatt ccggccgagg agcagggctt ccgcatcctg gggctgcccg agcacgagcc
     5821 gccggtgctc gcgctggcgg gagcggaccg cgtcgggacg ctgtatgccg cctatgaact
     5881 gcttgaagaa ctcggggttc gctggtacgg accaggcgag gtgaacgagg aggcgccgcg
     5941 gcgggagggg ttcgcgatcc ccgccctgga ccgcgtcgag gcgccgggct tccgcagccg
     6001 gggcttttgg gcctgggaag accgcgccac gcccgagttt attgagtgga tgggccgcaa
     6061 ccggatgaac ttctggacgg tcgaggatca gcagcgggcg caactgaagc tgcgcgggat
     6121 ccggctgacc tgcggcagtc atcatacgca gtggcggttc attcaccccg atcatccgta
     6181 tccgtatgat gtcgcgcggt tcaccgggga tgagaacaaa ccggccgatc cctaccggga
     6241 gggcgagtac cagggggacg tgaacggcga cggggcgctg agctacagcg aggtccatcc
     6301 ggagtggtac gggttgcaga agggcaagcg gagcttccgt ttcgagtatg atttcggcga
     6361 caacatctgc gattccaacg aggatggggt gaccgaattc tacaggaatc tggtgcagga
     6421 tctgatcgat ggccagtggc ggtgggccga cagcatcaac ttctggatgt tcgacagcgg
     6481 cgtctggtgc gagtgcgagg cgtgccaggc gctggggacg cccaccgacc gcaatctgcg
     6541 gctggtgcac cggctgcgcc aggcgatcga cgcggcgcgg gcggcggggc ggctccagcg
     6601 gccggtgcag atcgaatttc tggcgtattt cgacgtgatc gagccgccga cgcggccgtt
     6661 gcccgaggac tttgattacg acggctgcgt ggcgacgttc tttccgatcc ggcgctgtta
     6721 cgttcacgcg ttcgatgacc cggcctgcac ggaattgaac gcgccgctgc atgcgcagta
     6781 taccggctgg gcgacggacc cggagcggca ctaccggggg cagatcttca tcggcgaata
     6841 ttacaacgtc tcgttcttcc ggaatctgcc gctggtgctg gaccagattc tcgcgagcga
     6901 tattccctac tttcaccgga ccggcgcgcg gcacatgcac tacatgcatg cgccgggggg
     6961 gatgctgggc ccgcgcgcgc tgaccaactg gctgctcgcg cggatgctgt gggaaccgga
     7021 ggcggacgcg ggggcgctgc tggaggatta ctttacgggg cgctacggcg cggtcgcgcc
     7081 gacgatgcgc cggctgtacg atcacctgcg cacggggctg agcaatacga cccttctgaa
     7141 aagcgtgatg gcgcggcggc tgaacgaaca gcgccgggac ctgtttccgg accgccattt
     7201 gaagtacgag gaaacgcacc cggagaccga cgacggtccc gatttcgtgg agatcctgaa
     7261 ggagatggac gcggcggggc ggctgatggg cgaggcgctg cggacgcccg cgccgcggcg
     7321 ggtccggctg cggctgatgg aggatcagcg gatggtgcgc tacgccgatc acacggtccg
     7381 gctgtatgac cggctgctgg ccacgcagcg cgcgctggcg gcgggcgacc gggacgcggc
     7441 ggaggcggcc tacgccgaag ccacgatcca cgccgaccgg ttgcgggtcg atatcctgtc
     7501 gacgagcatg gcgtcgtcgc atgcgaacga caagaacggc ttggaggcga gcctggtctc
     7561 gggagtgtat gaggaaatcg gcaaggcctt caagaccggg ggggaagcgg cggcgcggta
     7621 acctgatgcc ccatctcccc gccctgccgg gatacggggg cgccggtggg tcaggcgagt
     7681 gcgtcctgac cgcccggccg gatcagggtg aatgcgcggg ctcgcccttg acgcccagcg
     7741 tgcgctcgag ccagtcgtag gccaggttct ggcggtcggg tgggaagctg tgcgggccgt
     7801 cgacgagata cagccgcagg agcgcggggg cgtcgtagag ggcatagacg ccgcgcacct
     7861 gctcgaaaac gtcgcgcagg ccgtcggtgt tggggaagat gtcgtcggcg agcgtgccga
     7921 aataaaagaa ggggcgcggg gcaaggcagg cgaggacctc gtgccagtcg aacggcgcgg
     7981 tgcggatgtc gtcggtgaaa aacccgagtc gcggcaggag gggcgtggca tggctccagc
     8041 gccagggggt ggggtcggcg cggaaggtat tgatgccgca actggcgacg acggcgcaga
     8101 cgcgcgggtc gaagacggcg cccatgactc cgccgtaggc gccgtgggaa tggccgatga
     8161 cggcgatgcg ctcggggtcg acttcggggc gggtttgcag gaagtcgacg atccgcgaga
     8221 agtcccagtt catccggccc aggacggacc aatcgggatg gcgccggaag aattcgtggc
     8281 tgttgtgata cggatcggtg ccggggggga tgcgttcgcc gaagccgggc gcgtcgtagg
     8341 cgatcgtgat caggccgcgg cgggccaggt cctcggcgaa gttcagatcg aagctcatgt
     8401 tgacgccggc cggttcctcc ttgccctgcg cgaccgtctg atgcaggacg atcacggcgg
     8461 ggaggggcgc ttgcgcggcg ggcaggggca ggaacagata ggcggggatc cggccgccgt
     8521 cgctgtcggc gttgatcatc aggtggatgc gttcgaagtc gggatcggca tggcggtcga
     8581 cttcgtaggc atccggcgcg accggttcca gattggtcgg ctcgccgatc acggcctgga
     8641 gcgtcgcgcg gaggcgctgg cgtttctcct cccactggtc ggcggttgag atggttgtgc
     8701 cgtcatcggc gagcagcagc ggcgcgaggt cgagccgacc ggcctgcggt gtttgcaggc
     8761 gggggggctc gggctgggcg atttcgcgcc aggtttcatg atcgaaaccc tcccaggcgt
     8821 gggccgcggc ggcgaggccg gctagcaggc aggcggccat caggccgcgc gagaggcgac
     8881 ggtcgtgttt aaggcagcgc atggcggttt ttctccttcc ttcgatcaag catccaaaga
     8941 accagtccca gcaggagcat ccccgcgccg acgaacaggc tggggagccc ctgctccagg
     9001 gaaacgaaca acaggacaac cagcagcagc agcgccccca gcgcggcggt gacgagggcg
     9061 atgaacggga ggggggagaa atcggccggc ccggtcgcgg ccggcggcgg cgcggccggc
     9121 tcgggttcgg tcagccgcgt gaagaaggcg tcggtccgcg cgcgctgggc cgccggatcg
     9181 ggtcgcagcc aggagccgac cccca
//
LOCUS       DAOPCO010000156         9185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7119_length_9185_cov_4.642497, whole genome shotgun sequence.
ACCESSION   DAOPCO010000156 DAOPCO010000000
VERSION     DAOPCO010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7119_length_9185_cov_4.642497"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLS90_11125"
     CDS             <1..427
                     /locus_tag="PLS90_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-mannonate epimerase"
                     /protein_id="HPK02997.1"
                     /translation="SVYRTRMAIADGGELLVLAPGLRAFGEDPGIDRLIRKYGYFGTP
                     ATLRQVEGQPELQTSLAAAAHLIHGSSEGRFRIVYAPGKLSREEIESVGYEWADLAQA
                     LRRYDPDRLVEGWNERDGEEFYFIRHPGLGLWSQSPVRE"
     gene            complement(724..3132)
                     /locus_tag="PLS90_11130"
     CDS             complement(724..3132)
                     /locus_tag="PLS90_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPK02998.1"
                     /translation="MTPNRRAAAMPPRPAGEKPRQTMELASHVERWYLAQSGRQLFYP
                     PLEKIPVIEVPNFPSLGRLTALRFVEWVLEHPEGVVSLPTGKTPEYFIRWTQQFLEMW
                     TSRETQRQLAEIGLPAERKPDLRGLRFVQIDEFYPIEPTQHNSFHYYVNKYYIRGFGL
                     DPEKALLIDPSRIGLDGETPLDSIFPDGKVDLTLRARNAASELERRQQRVIEAVDQFC
                     TEYEERIRELGGLGFFVGGIGPDGHIGFNARGSHHQSPTRLTQTNYETEAAAAGDLGG
                     IEISRNKPVITIGLGTITYNREAVVIIFAAGDAKARVAADAIQQERHVRYPATVLQGM
                     PNARFYLTQGATLHLEERRFENILKAERIPDELLERAVIDRALALGKRLEELKPAMAK
                     EDRLLSRVLERTGRKLDEAAAWTRERMLAKIERGLDDLSDQVILHTGPHHDDIMLGYM
                     PYVWHLVRRVSNRNYFYVLTSGFTAVTNGFMAELLEELRGLLEADAFAADQAAGELDR
                     ENRMARAREIFHYLDGIAARDPRMCARAQARRTLVNLMSLYEDEDLDNIRQRIAENLN
                     YLRTLYPGKKDIPIIQKLKGMQREFEEELIWAYVGTQPEQVNHARLGFYTGDIFTQQP
                     TVERDVAPVLEHIRRVQPTIVSLAFDPEGSGPDTHYKVLQVLHEAMARYRDETQREPI
                     VWGYRNVWYRFHPAEANVFIPATLNTMAIMEHSFMHCFGSQRNASFPSYEYDGPFCHQ
                     AQKLWVEQFRMVQTCLGERFFLENQSPRLRATRGYVFIKAMTMEEFSGKARRLAELTE
                     GAVE"
     gene            complement(3190..6090)
                     /locus_tag="PLS90_11135"
     CDS             complement(3190..6090)
                     /locus_tag="PLS90_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPK02999.1"
                     /translation="MSKTINLRQFEQDLRQRMRGEVYFDQVTRGLYSTDASIYQEMPI
                     AVCAPRDEEDVAAALRVARDHQAPVLPRGGGTSLAGQAVATALVLDFSRYMNKILDLD
                     LEQGWVRVQPGVVRDELNAKLAAHGMEYAPDPATANRANIGGMIGNNSSGTRSIIYGK
                     VVDHILQTVVHLADGTRLDLHAWSAAEYEARSQGESREAEILRGFRKLIETHRDEIAH
                     RFPKVMRRVGGYNLDQFIQPDAPEGWNLAKLVTGSEGTLATTVEARLNLVRLPRATAI
                     CVCHFADLVESIRAVPSIVRHGPSAVEILDRQTLHLARRNLSTAPLCNFIQGDPAAIL
                     IVEFFGDSTEEVREKCETLGRTLASDGFGYAWPVYTDAEEKAKVWNVRKNGLGLMLGM
                     KGDRKPIPFIEDACVPVEVLPEYITQVLEICRRNEAPVIMYAHASVGVIHVRPILDLR
                     RRDEIERMKRIAEEAFQLVVRYHGSWSSEHGDGYVRSPWMERFYGPRIYGAFKEVKSL
                     FDPEWRMNPGKILEAPEMDHDLRYGTEYHAQANGTAFHYREDGGFARAVEMCTGVGAC
                     RKTLGGTMCPSYMATRDEEHSTRGRANALRLAMTGRFGPDGMTSRRMYEVFDLCLSCK
                     GCKAECPSNVDVARLKSEFLQRYHAAHGTTLRDRLIRDSASHAAAAAGMAAPLVNWIQ
                     ASAPFRLLAEKAAGIDRRRKLPGFALRTFPAWHARRNAQANGGTGRPRVVLFDDTYMN
                     YHEPNVGRSAVELLEGCGYEVQLARAGCCQRPAISHGFLDEAREKGERTLRALDAYIQ
                     QGLPVLVCEPSCASALVDDLPDLIEDVELGHRVKENVMMIDVFLDREMQAGRIAPRLG
                     SSSAEILVHGHCHQKALFGTGAMKRILGRVEGLAVSEVDSGCCGMAGSFGYEKEHYEL
                     SRRIAEDRLLPAIRSRKPGATIVACGFSCRHQIQDFAGEQAYHWVETVHAQT"
     assembly_gap    6111..6210
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6445..7557)
                     /locus_tag="PLS90_11140"
     CDS             complement(6445..7557)
                     /locus_tag="PLS90_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HPK03000.1"
                     /translation="MQELRAIPRYLMGPGPSDADARVLRAMATPLIGHLDPQFIQIMD
                     EIKAMLRQVFRTGNELTFPVSATGSAGMETVLVNLLEPGDTAVVCINGVFGNRMADIV
                     ERCGARVVRVTAPWGKPIDAVEVRAALASCCPKLLAIVHAETSTGVLQPLEEISGLTH
                     DSGALFVVDAVTSLGGVDVRVDDWGIDAIYSGTQKCLSAPPGLSPVTFSPAALECIEK
                     RKVKPQSWYLDVGMVRNYWEGAKRTYHHTAPISANYALHEALRIVLEEGLEARFERHR
                     RNHERLRDGLEELGFRFVVEPEYRLPQLNTVWLPDWLEDGPGRARLLEEYNIEVGGGL
                     GDFAGKVWRIGLMGNSSTPNHVDMLLAALRRMRDEG"
     regulatory      7962..8064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8100..>9185
                     /locus_tag="PLS90_11145"
     CDS             8100..>9185
                     /locus_tag="PLS90_11145"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPK03001.1"
                     /translation="MSIAARSAATERIQLDSALRAPFPRSEKIYVAGSRPDVRVPMRR
                     VELSPTRSANGHEQNPPVYLYDTTGPYTDPNVELDLARGLPPLRLPWILERDDTEEQP
                     GYASNYARARATDPALEGIRFERRRPPRRAKPGRCVTQMHYARKGIITPEMEFIAIRE
                     NMRRDELHERGVLASQHPGQSFGAAIPRRITPEFVRDEVARGRAIIPANINHPESEPM
                     IIGRNFLVKINANIGNSAVSSSIAEEVEKMTWATRWGGDTVMDLSTGAHIHETREWIL
                     RNSPVPIGTVPIYQALEKVGGRPEELTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIP
                     LTARRVTGIVSRGGSIMAKWCLAHHREN"
BASE COUNT         1499 a         3568 c         2410 g         1608 t
ORIGIN      
        1 gtccgtctac cggacgcgga tggccatcgc cgacggcggc gagctcctcg tcctcgcccc
       61 cggcctgcgc gccttcggcg aggaccccgg catcgaccgc ctgatccgca aatacggcta
      121 cttcggcacg cccgccacgc tgcgccaggt cgagggccag cccgagttgc agaccagcct
      181 ggccgccgcc gcccatctga tccatggctc cagcgagggc cgcttccgca tcgtttatgc
      241 ccccggcaag ctctcgcgcg aggaaatcga gtcggtcggt tatgaatggg ccgacctcgc
      301 ccaggcgctc cgccgctacg atccggaccg cctcgtcgaa ggctggaacg aacgcgacgg
      361 cgaggaattc tacttcatcc gccaccccgg cctcggcctc tggagccaat ccccagtccg
      421 cgaataaccc tcactctaaa atcgtccctc gtcctcgtcc ctcgtcctcg agacccctcc
      481 cgaacgactc cccattcttg tcccttctcc ttcgttgtga accgcaggct catgagcaac
      541 tcgtaatcgc ccccaacggc cgccccagaa ttcggctctc ggctctcgtc cctcgtcctc
      601 ggctctcgaa attctctttt ctcccgaccc ggcgccctct cccccgtttc cgttcctgcc
      661 ccgcttttcg tgccctttcg cgcctttcgt gatcctctcg acgcccctcc cccctgccca
      721 cgcctactcc acggcccctt ccgtcaactc cgccagccgc cgcgccttcc ccgaaaactc
      781 ctccatcgtc atcgccttga tgaacacgta gccccgcgtc gcgcgcagcc gcggactctg
      841 gttctccagg aaaaaccgct cgcccagaca cgtctgcacc atccggaact gctcgaccca
      901 cagcttctgc gcctgatggc agaacggccc gtcgtattcg tagctcggga acgatgcgtt
      961 ccgctgcgat ccgaagcagt gcatgaagga atgctccatg atcgccatcg tattgagcgt
     1021 cgccgggatg aagacgttcg cctccgccgg gtgaaaccga taccagacgt tgcggtaacc
     1081 ccagacgatc ggttcccttt gcgtctcatc gcggtagcgt gccatcgcct cgtgcagcac
     1141 ttgcagcacc ttgtagtgcg tgtccggccc ggaaccctcc ggatcgaacg ccagcgaaac
     1201 aatcgtcggc tgcacccggc ggatatgctc cagcaccggc gccacgtcgc gctccaccgt
     1261 cggctgctgc gtgaagatat cgcccgtgta aaaccccagc cgcgcgtgat tcacctgctc
     1321 cggctgcgtg ccgacatacg cccagatcag ctcctcttcg aactcgcgct gcatcccctt
     1381 cagcttctgg atgatcggga tgtccttctt ccccggatac agcgtgcgca ggtaattcaa
     1441 attctccgcg atgcgctggc ggatgttgtc caggtcctcg tcctcgtaca ggctcatcag
     1501 gttgaccagc gtgcggcgcg cctgcgcgcg ggcgcacatc cgcgggtcgc gcgcggcaat
     1561 cccgtccagg tagtggaaaa tctcgcgcgc gcgggccatg cggttctcgc ggtccagctc
     1621 gcccgccgcc tgatccgccg cgaacgcgtc cgcctccagc agcccccgca gttcctccag
     1681 caactccgcc atgaacccat tggtgacggc ggtgaatccg ctcgtcagca cataaaagta
     1741 attccggttc gagacgcgcc gcaccaggtg ccagacgtag ggcatgtagc ccagcatgat
     1801 gtcgtcgtga tgcgggccgg tgtgcaggat cacctgatcc gacaggtcgt ccagcccccg
     1861 ctcgatcttg gccagcatcc gctcgcgcgt ccacgcggcg gcttcgtcca gcttgcggcc
     1921 cgtgcgctcc agcacccggc tcagcagccg gtcctccttc gccatcgccg gcttgagctc
     1981 ctccagccgt ttgcccagcg ccagcgcgcg atcaatcacc gcccgctcca gcagttcgtc
     2041 cgggatccgc tccgccttca ggatgttctc gaaccgccgc tcctccagat gcagcgtcgc
     2101 cccctgcgtc agataaaagc gcgcgttggg catcccctgc agcaccgtgg ccgggtagcg
     2161 cacgtgccgc tcctgctgaa tcgcgtcggc cgcgacgcgc gccttcgcat cccccgccgc
     2221 gaagatgatc accaccgctt cccgattgta cgtgatcgtc cccaggccga tcgtgatcac
     2281 cggcttgttg cgcgagatct cgatcccccc caggtcgccc gccgccgccg cctccgtctc
     2341 gtaattcgtc tgcgtcagcc gcgtcggcga ttggtggtgc gaaccccgcg cgttgaaccc
     2401 gatgtgtccg tccggaccga tcccgcccac aaagaatccc agaccgccca gctcgcggat
     2461 ccgctcctcg tattcggtgc agaactgatc gaccgcctcg atcacccgct gctggcgccg
     2521 ctccagctcg ctcgccgcgt tgcgcgctcg cagcgtcaga tccaccttcc cgtcgggaaa
     2581 tatcgaatca agcggcgtct ccccgtccag cccgatccgc gacgggtcga tcagcagcgc
     2641 cttctccgga tcaagcccga acccgcgaat gtagtacttg ttcacgtagt aatggaagct
     2701 gttgtgctgc gtcggctcga tcggatagaa ctcgtcgatc tgcacaaacc gcaatccgcg
     2761 caggtcgggc ttgcgttcgg ccggcaggcc gatctccgca agctgcctct gcgtctcgcg
     2821 cgacgtccac atctccagaa actgctgcgt ccagcggatg aaatactccg gcgtcttccc
     2881 cgtcggcagg ctcaccaccc cctccggatg ctccagcacc cactccacaa agcgcagcgc
     2941 cgtcaaccgg ccgaggctgg gaaagttcgg cacctcgatc accgggatct tctccagcgg
     3001 cgggtagaac agttgtcgcc cggactgcgc cagataccag cgctccacgt gcgacgccag
     3061 ttccatcgtt tggcgcggct tctcgcctgc ggggcgcggc ggcatggcgg cggcgcggcg
     3121 gttcggcgtc atgacttagg ctcccgaatc ggtacggtgg ggcaggaatc gcccggcagg
     3181 ccgtccgcgt cacgtttgcg catgaacggt ttcgacccag tggtaggcct gttcgccggc
     3241 gaaatcctga atctggtggc ggcaactgaa cccgcacgcc acgatcgtcg cccccggctt
     3301 gcgcgaccgg atcgccggca gcagccggtc ctccgcaatc cgccgcgaca gctcatagtg
     3361 ctccttctca tacccgaaac tgccggccat cccgcagcag ccgctgtcga cctcgctcac
     3421 cgccagtcct tccacccgcc ccagaatccg cttcatcgcc cccgtgccga acagcgcctt
     3481 ctggtggcaa tggccgtgga ccaggatctc cgcgctgctc gagcccagcc gcggcgcgat
     3541 ccgccccgcc tgcatctccc gatccaggaa aacatctatc atcatcacgt tctccttgac
     3601 gcggtggccc agctccacgt cctcgatcag atcgggcaga tcatccacca gcgccgacgc
     3661 gcagctcggc tcgcacacca gcaccggcag cccctgctga atgtacgcat ccagcgcccg
     3721 caacgtgcgc tcccctttct cgcgcgcctc atccaggaat ccgtgcgaga tcgccggccg
     3781 ctggcaacac cccgcccggg ccagctgcac ttcgtagccg cacccttcga gcagttcgac
     3841 cgccgagcgc cccacattcg gctcgtggta gttcatgtag gtgtcgtcga acagaacaac
     3901 gcgcgggcgc cccgtcccgc cgtttgcctg cgcgttgcgc cgcgcatgcc aggcggggaa
     3961 cgtccgcagc gcgaacccgg gcagcttgcg ccgccgatcg atccccgccg ctttctccgc
     4021 cagcagccgg aacggcgcgc tcgcctggat ccagttcacc agcggcgccg ccatccccgc
     4081 cgccgccgcc gcatggctcg ccgagtcgcg gatcagccgg tcgcgcagcg tcgtcccgtg
     4141 cgccgcatga taccgttgca gaaactccga cttgagccgc gccacatcca cgttgctcgg
     4201 acactccgcc ttgcacccct tgcacgacag gcacaggtca aacacttcat acatccgccg
     4261 gctcgtcatc ccgtccggcc cgaaccgccc ggtcatcgcc agccgcaacg cattggcccg
     4321 cccccgcgtc gagtgctcct cgtcgcgcgt cgccatgtag ctcgggcaca tcgtcccgcc
     4381 cagcgtcttg cggcaggccc ccacgcccgt gcacatctcc acggcgcggg caaatccccc
     4441 atcctcgcgg tagtggaacg ccgtgccgtt cgcctgcgcg tgatactccg tcccatagcg
     4501 caggtcatga tccatctccg gcgcctcgag aatcttcccc gggttcatcc gccattccgg
     4561 gtcgaacagc gacttcacct ccttgaacgc cccgtaaatc cgcggtccgt aaaaacgctc
     4621 catccacggg ctgcgcacgt agccgtcgcc atgctcgctg ctccacgacc cgtggtagcg
     4681 cacgacgagt tggaacgctt cctcggcgat ccgcttcatc cgctcgatct cgtcgcgccg
     4741 ccgcaggtcc agaatcggcc gcacgtggat cacccccacg ctcgcgtgcg catacatgat
     4801 caccggcgcc tcgttgcgcc ggcagatctc cagcacctgc gtgatgtact ccggcaacac
     4861 ctccaccggc acgcacgcgt cctcgatgaa cgggatcggc ttgcggtccc ccttcatccc
     4921 gagcatcagg cccagcccgt tcttgcgcac gttccagacc ttggctttct cctcggcatc
     4981 ggtatagacc ggccacgcgt aaccgaaccc gtcgctcgcc agggtgcggc cgagcgtttc
     5041 acacttctcc cgcacctcct cggtcgagtc gccgaaaaac tcgacgatca gaatcgcggc
     5101 cggatcgccc tgaatgaaat tgcacaacgg cgccgtgctc aaattccgcc gcgccaggtg
     5161 cagcgtctgc cgatccagaa tctccactgc cgagggtccg tgcctcacga tgctcggcac
     5221 cgcgcggatc gactccacca agtcggcgaa atggcacacg cagatcgccg tcgcccgcgg
     5281 cagccgcacc aggttcagcc gcgcttccac cgtcgtggcc agcgtccctt cgctccccgt
     5341 caccagtttg gccagattcc aaccctccgg cgcatccggc tgaatgaact ggtccaggtt
     5401 gtaacctccg acgcggcgca tcaccttcgg gaaccggtgc gcgatctcat cccggtgcgt
     5461 ctcaatcagc ttgcggaagc cgcgcaggat ctcggcttcc cggctttccc cctggctgcg
     5521 cgcctcgtac tccgccgcgc tccaggcgtg cagatccagc cgcgtcccgt ccgccagatg
     5581 caccaccgtc tgcagaatgt gatccacgac ttttccataa atgatgctgc gcgtgcccga
     5641 cgagttgttc ccgatcatcc ccccgatatt ggcgcggttc gccgtcgccg gatccggcgc
     5701 atactccatg ccatgcgccg ccagcttcgc gttcagctca tcgcgcacca cgcccggttg
     5761 cacccgcacc cagccctgtt ccagatccag gtccaggatc ttgttcatat accgcgaaaa
     5821 atcgagcacg agcgccgtcg cgaccgcttg cccggcgagg ctcgtcccgc ccccgcgcgg
     5881 caggaccggc gcctggtgat cccgcgccac gcgcagcgcg gccgcgacgt cctcctcgtc
     5941 gcgcggtgcg cacaccgcaa tcggcatctc ctgataaatg ctcgcatcgg tcgaatacag
     6001 accgcgcgtc acctgatcaa aataaacctc cccgcgcatc cgctggcgga ggtcctgttc
     6061 aaattgacgg agattgatgg tcttgctcat caggctgggt ctctatctcc nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn accgtcctac ttcacctgcc cttcacctgc
     6241 cttccgcccc tcacctctcc gttcgtcggc cctcgtcctc gcccccaacg gccctcccaa
     6301 aaaactcggc tctcggctct cgtccctcgt cctcggctct cgaaaacccc aggtgcagcg
     6361 cagcggaacc tggggcaacg cccccaaata tccccgtccc cgaagggggc gcaccgcgat
     6421 gcccccaggc catcaccctg acattcagcc ctcatcccgc atccgccgca gcgcggccag
     6481 cagcatgtcc acgtgattcg gcgtgctgct gttccccatc agcccgatgc gccacacctt
     6541 ccccgcgaag tcgcccagcc cgccgccgac ctcaatattg tattcctcca gcagccgcgc
     6601 gcgacccggc ccatcctcga gccagtcggg cagccagacc gtattgagct gcggcaggcg
     6661 atactccggc tccaccacga accggaaacc cagctcctcc agcccgtcgc gcaggcgctc
     6721 atgattccgc cggtggcgct cgaatcgcgc ctccagccct tcctccagaa cgatccgcag
     6781 cgcttcatgc agtgcgtagt tggccgagat cggcgccgta tgatgatacg tccgcttcgc
     6841 cccttcccag tagttgcgca ccatcccgac atccagatac cagctctgcg gcttcacctt
     6901 gcgcttctcg atgcattcca gcgccgccgg actgaacgtc accggcgaca ggcccggcgg
     6961 cgcgctcaga cacttttgcg tcccgctgta aatcgcatcg atcccccaat cgtccacacg
     7021 gacatcgacg ccccccagcg aggtgaccgc atccaccacg aacagcgccc ccgaatcatg
     7081 cgtcagcccg ctgatctcct ccagcggctg caacaccccg gtcgacgtct cggcatgcac
     7141 gatcgccagc agcttggggc agcatgaggc cagcgcggcc cgcacctcga ccgcgtcgat
     7201 cggcttgccc cacggcgccg tcacgcgcac gacccgcgcc ccgcagcgtt ccacgatgtc
     7261 cgccatccga ttgccgaaca caccgttgat gcagactacc gccgtatcgc ccggttcgag
     7321 caggttcacc agcacggtct ccatccccgc gctgccggtc gccgataccg gaaacgtcag
     7381 ttcattcccc gtccggaaga cctggcgcaa catcgccttg atctcatcca tgatctggat
     7441 gaattgcggg tccagatgac cgatcagcgg cgtggccatc gcccgcaaca cgcgcgcgtc
     7501 ggcgtcgctc ggacccggac ccatcagata gcgcgggatc gccctcaatt cctgcatctc
     7561 atccctctct ctccgactgc ggctcgatct caagccttca agtgtaaaag ccccaattcc
     7621 gattctccat ggatttgttc cccccagaat tgtcccccgt ctttcgtccc tcgtcgccgg
     7681 ctctcgaaac cccgctattc atcccctcga cttgaaccac ctggtcccga gcaactcgca
     7741 atcgagttct ccctcccgtc ccttatgtct cacgccagaa ccgtaagctc atcctgactt
     7801 ccctcagtcg agtcatcgct ctcctctccc acgaacccgt ctcaggcaaa ctctgatcgt
     7861 tgcggaatcc cgcatccgca tccgcccctc accccccacc tccgccccat cgccgcataa
     7921 tcccttgatc ccggtcgccg cgacggcgta agctggattt gcgttatagc tgggggtgcc
     7981 ccgtcgccga caggacgggg ctgagagata ccctttgaac ctgatccggg tgatgccggc
     8041 gtagggaagc taacctgact cggccttccc ttgaacattt ctctgccaag gtcagtccga
     8101 tgagtattgc ggcacgttcc gcggcaaccg agcggatcca gttggacagc gccctccgcg
     8161 cgcccttccc ccgttccgaa aaaatctacg tcgccggcag ccggcccgat gtgcgcgtcc
     8221 ccatgcgccg cgttgagctc tcacccaccc gctcggccaa cggccacgaa cagaatcctc
     8281 ccgtttatct ctacgatacg accggcccct ataccgaccc gaacgtcgag ctggatctgg
     8341 cgcgggggct cccccccctg cgcctgccgt ggatcctgga acgcgacgac accgaggaac
     8401 agcctggcta cgcgtccaat tacgcccgcg cgcgcgcaac cgaccccgcg ctggagggca
     8461 ttcgctttga gcgccgccgc cccccccgcc gcgccaaacc cggccgctgc gtcacccaga
     8521 tgcactacgc ccgcaagggc atcatcaccc ccgaaatgga attcatcgcg atccgcgaga
     8581 acatgcggcg cgacgagctc cacgagcgcg gcgtcctcgc ttcgcagcac cccggccagt
     8641 cgttcggcgc ggcgatcccc cgccggatca cccccgaatt cgtccgcgac gaggtcgccc
     8701 gcggccgcgc catcatcccc gccaacatca atcatcccga gtccgagccg atgatcatcg
     8761 gccgcaattt cctcgtcaag atcaacgcca acatcggcaa ctccgccgtc agcagctcga
     8821 tcgccgagga agtcgaaaaa atgacctggg ccacccgctg gggcggcgat accgtgatgg
     8881 acctctccac cggcgcccat atccacgaga cccgcgagtg gatcctgcgc aacagccccg
     8941 tccccatcgg caccgtcccg atctaccagg cgctcgaaaa agtcggcgga cgccccgagg
     9001 agctgacttg ggaaatcttc cgcgacaccc tcatcgaaca ggccgaacag ggggtcgatt
     9061 actttacgat ccacgccggc gtcctgctgc gctacatccc cctcaccgcc cgccgcgtca
     9121 ccggcatcgt cagccgcggc ggctcgatca tggccaagtg gtgcctcgcc caccaccgcg
     9181 aaaac
//
LOCUS       DAOPCO010000157         9182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7124_length_9182_cov_5.243125, whole genome shotgun sequence.
ACCESSION   DAOPCO010000157 DAOPCO010000000
VERSION     DAOPCO010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7124_length_9182_cov_5.243125"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLS90_11150"
     CDS             <1..616
                     /locus_tag="PLS90_11150"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPK03002.1"
                     /translation="CLGGIEDGHGAGLSDSETADHLTDPAQAEDFVAKTGLDALAVAI
                     GTSHGAYKSGRKDPKTGEVLPPALAMDRIHEINKRMPDCHMVMHGSSSVPRELVEIIN
                     KYGGKMPGTFGIPIAEIQDGIKHGVRKINVDTDSRLAMTGAIRKVLFESPEKFDPRDY
                     LKPAREAMKEVYIARMKAFGQAGHATDYKQMTLDEMKERYTAKV"
     gene            complement(833..2365)
                     /locus_tag="PLS90_11155"
     CDS             complement(833..2365)
                     /locus_tag="PLS90_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03003.1"
                     /translation="MSARLALGLALILFGCYGLTASKLRFGYEGANIDQAEALLRGDW
                     VPRLKGGIAPYTQGGLADVAAYFPFAALKLGLDRTGLLFGLRQLIYPFVVPLYTVALL
                     LVLFGLGRDLYGRSAPALALTLVAGLATMLWPYAKFGMETQQTLWTLAAVWALVRYHR
                     VPAAGAAAAFGIALALLMLTKITGPLHAAALLLAAGWIVARRGARPGGGAHVLLVILL
                     TAGGAAIFLFSNRWRYGGWLWAGRYGMGFELSRYPLWEALWAVAASPGESLLLFCPPL
                     LISLWFIGSFWRRFPVMRPILVLMLAVGILHLRNRPWADETWGPRRLHYLVPLLALPL
                     GAWFERLPDLRRPVRWLGWAVVAAGFGVQLIAVAFDYTAQAFVLGRTPLYSQENTVWD
                     PQLSPVRFNLHLLRSAAHRAATGEAIPFVYERHYLPWTKPDNPPPPESFSMEGYDHFD
                     LWYFQQRADWPDRPYWFHSPSSFVVPLLGLMIVGGILLLATTREAKIEMRRSKYETNS
                     SP"
     gene            complement(2560..5460)
                     /gene="infB"
                     /locus_tag="PLS90_11160"
     CDS             complement(2560..5460)
                     /gene="infB"
                     /locus_tag="PLS90_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPK03004.1"
                     /translation="MRIYDLAKSIGQQLNITVKSGDLADEFRTLPEFAEIHDTIKSHA
                     SSVDDRFARVMFEIYARRQREGATTPAAAPAAKPAAPKVVAPRPAGPGRPSAPAQRPA
                     PPAPVARPAAPAPPPLRRPAAPPRPAPITPGPAGGPRPVVPIPGRRPAPIQPIGKAPA
                     APVVEPPPPIAPPRPKAEAPTPSAAPAAPGEQRTRPAAAPRPGKLRPSGLATELKSSS
                     GTTGVSAAPETGRPAPGVSRIGQPTAPAAPKFSADSPFLKPPARRERPAAGGERPGAD
                     ARRPGGRRIDAKEIPLSPEQLTALPEIGVMKLKPGGKRGRKPGEAERDEKAKAAAAKK
                     GVRPSRVYGDADEFRRPIKPGAKRTKTPPDRRRDEPKVPQKVSELTLTGPVTVSEFAE
                     KMGLPPAELIKQAFLKGKAITINQLMDFELAEELAIDNDVDLKIQLEADETDVAEYLQ
                     AEDRPEDLRPRPPVVTIMGHVDHGKTSVLDYYRKSAVAEGEYGGITQHIGAYQIKSPN
                     GGTIVFLDTPGHEAFTAMRARGVKCTDIVVLVVAADDGVMPQTIESINHAKAAGTPLI
                     VAINKMDLPQANPERVRQELMQHSVLSTQLGGDTEFAEISAKTGRGMDDLLEIIQLQA
                     ELLELKANPNRPAQAVVIESHTDPMRGSVATVLVQRGTLRVGQYFVVGQEHGRVRAMY
                     DDRGRPVQATTLAQPVELIGLSGTPEVGETLLVMEDERKAREIAERREQRRRLVELGT
                     TRHISLEGLHDRIAEGEIKDFNVILKADVQGSVEAIIQALEKLHNEEVRVRVLHSGTG
                     SINESDVNLAMASDAVIIGFNVRPEPGANDLATREGIDLKLYRIIYELLEDVEAALVG
                     QLKKRYQEQTLGRAEVRKVFRVSRVGTVAGCMVVSGEISRNAQARLLRDGKIVWEGRI
                     DSLRRVKDDVARVASGYECGIGLERFQDIKEGDVIEAFALEEIPAELTRSSS"
     gene            complement(5681..7159)
                     /gene="nusA"
                     /locus_tag="PLS90_11165"
     CDS             complement(5681..7159)
                     /gene="nusA"
                     /locus_tag="PLS90_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPK03005.1"
                     /translation="MDSNSLKAFVRLISKEKDLPPEILKEAIEHAILSASRKQLLNYR
                     DAVANLDLENGTLNVIVTKTIVEEGRKGHPREIGLKEARKLTGDRKLKVGQDVRVPIE
                     PSEFGRIAAQSVRQMVMQRLRDAERAKIFEEFKDKERMMVTGIVQRFERRDVILNIGR
                     AEGVLPASEQPMGARYKFNDRLKVLIARVEDTPKGPNIILTRKSPQLVIELFRQEVPE
                     IADGTVQIVGIAREAGVRTKIAVKSVNSDVDPVGACVGMKGSRVQMVVRELENEKIDI
                     VPWSSEASTFIRNALNPAKIVSIELDNEARHAHVVVAQGNLAIAIGRKGQNTKLAARL
                     TGYRLDIRSENEDELAYEEIQRRYLEDFLNQIEELSELYAQAILRSSFNSVEKIAKAD
                     PAKLLPFAGGNEDLVQTLIVGAEDYLNALREMEVERRRLREEEAAAEAANPEGAEQSG
                     QTEEAGESQPEAETGAGDQDQESASGEPAEAENQEVTDQRAG"
     gene            8034..8636
                     /locus_tag="PLS90_11170"
     CDS             8034..8636
                     /locus_tag="PLS90_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03006.1"
                     /translation="MAFNEARVSRGFSLACWGLALILFDLPWRRWVIPPDFFGWIVLT
                     IGLFWLQPLGARIQKAMWIAGAMIALSLPELLGYRRELLFHLGRPYYIDLLFPLPQLL
                     LIAVVLLLLHAIIDRANSLEHVELSHDTGVRRNLFLSWFVVYTLALWTSLFMPSLHRY
                     LMAIPWLYIIVILVLLILLLRRASEELVQAEATASARPSR"
     gene            8678..>9182
                     /locus_tag="PLS90_11175"
     CDS             8678..>9182
                     /locus_tag="PLS90_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK03007.1"
                     /translation="MLSFLLRRLAQSLAVLLAISILVFVMMYCLGDPVATLLPQNASQ
                     RQRAELRAALGLDRPLPVQYLIYVGNLLRGDFGLSYHQGRPVGEMLAERAPATLELAC
                     VAVLFSVLIGVPLGVLAGARARHPLSRLVLGGSLLGISVPSFWLGLLLMMLFGVLLGW
                     LPPMGRGR"
BASE COUNT         1501 a         3083 c         3015 g         1583 t
ORIGIN      
        1 ctgcctgggc gggatcgagg acggccacgg cgccggcctg tccgacagcg agacggccga
       61 ccacctgacc gaccccgcgc aggccgagga tttcgtcgcc aagacgggcc tggacgcgct
      121 cgcggtggcg atcggcacca gccacggggc gtacaagtcg ggccgcaagg accccaagac
      181 cggcgaagtg ctgccgcccg ccctggcgat ggaccggatc catgagatca acaagcggat
      241 gcccgactgc cacatggtga tgcacgggtc gagctcggtg ccgcgcgaac tggtggagat
      301 tatcaacaaa tacgggggca aaatgcccgg gacgttcggc atcccgatcg cggagatcca
      361 ggacgggatc aagcacggcg tgcgcaaaat caacgtcgac accgacagcc ggctggcgat
      421 gaccggcgcg atccgcaagg tgctgttcga atcgcccgaa aaattcgacc cgcgcgacta
      481 tctgaagccg gcccgcgagg cgatgaagga agtctacatc gcccgcatga aggccttcgg
      541 ccaggccggc catgcgactg actacaagca gatgacgctg gacgagatga aggaacgcta
      601 cacggcaaaa gtgtaagcga gccgtcagac aacggaacct gcgcaaagcg gcggatcgaa
      661 agatccgccg cttttgtgtt ggacgaaaca agcggaaaat atcgcaattc aaagatcgaa
      721 attcgcaaca atatcgaatt tctaatgaat acactgagaa acgatgacta aagattcagg
      781 cgatttgcat tggtcaccaa tcaatcgact gcaaccgtcg cgcgattcca atttatggac
      841 tggaatttgt ttcgtatttc gatcttcgca tttcgatctt cgcttccctt gtcgttgcca
      901 acaggagtat cccgcccacg atcatcagcc ccagcagcgg gaccacgaac gacgacggcg
      961 aatggaacca gtagggccgg tcgggccagt cggcgcgctg ctgaaaatac cacagatcga
     1021 agtggtcata gccttccatg gaaaagcttt ccggcggcgg cgggttgtcc ggcttcgtcc
     1081 acggcagata gtggcgttca tagacgaacg ggatcgcctc gccggtcgcg gcgcgatgag
     1141 ccgctgaccg cagcagatgc aggttaaagc gaacgggcga aagctgcggg tcccagacgg
     1201 tgttttcctg cgaataaagg ggcgtgcggc cgaggacgaa ggcctgcgcg gtatagtcga
     1261 atgcgacggc gatgagctgc acgccgaagc cggccgccac gaccgcccag ccgagccagc
     1321 ggacggggcg gcgcagatcg ggaagccgct caaaccaggc gcccagcggg agggccagca
     1381 gcggaacgag gtaatgcagc cggcgcggac cccaggtttc gtcggcccac ggccggttgc
     1441 gcaggtgcag gatgccgacg gccagcatca ggaccaggat cgggcgcatc accggaaacc
     1501 gccgccagaa cgacccgata aaccacagcg aaatcagcag cggtgggcag aacagcagca
     1561 gactttcgcc cgggctggcg gcgacggccc acagcgcctc ccagagaggg tagcgcgaca
     1621 gttcgaagcc catgccgtag cgcccggccc acagccagcc gccgtagcgc cagcggttgc
     1681 tgaagaggaa gatcgccgcg ccccccgccg tcagcaggat cacgagcaac acatgcgcgc
     1741 cgccgccggg gcgggctccg cgccgcgcga cgatccagcc cgcggcgagc aggagcgccg
     1801 cggcgtgcag cggaccggtg atcttcgtca gcatcagcag cgccagcgcg atgccgaacg
     1861 cggcggcggc gcccgccgcg gggacgcgat ggtaccgcac gagcgcccag accgccgcca
     1921 gcgtccagag cgtctgctgc gtttccatcc cgaacttggc gtagggccag agcatcgtcg
     1981 cgaggccggc aacgagcgtg agcgccagcg ccggggcgga gcgcccgtat aagtcgcggc
     2041 cgagcccgaa caggaccagc agcagcgcca ccgtgtacaa aggcacgacg aaggggtaaa
     2101 tcaattgtcg caagccaaag agcaggccgg tccggtcgag accgagcttc agcgcggcga
     2161 atgggaaata ggcggcgacg tcggcgagtc ccccctgagt atagggggcg atgccgccct
     2221 tcagccgcgg gacccagtcg ccgcgcagga gggcctcggc ctggtcgatg ttcgcccctt
     2281 catagccgaa gcgcagtttg gaggcggtca ggccgtaaca gccgaacagg atcagggcga
     2341 ggccgagcgc cagacgcgct gacacggggg acgggcgggg accggcggcg ggcagcatgg
     2401 aacgtagcgt gcggcaacgc gcccgttttg gcaactgcta tggcggggga gggccggctc
     2461 aggcggaagg gaaggagcgg tccggcgcgg tagagccatg aagaaccgac cgtcagggcc
     2521 gatcacgggt gcatcaaccc ttgcttgcgc ggcatcaact caactggaag accgcgtcag
     2581 ttcggcggga atttcctcca gcgcgaacgc ttcgatgacg tccccctcct tgatgtcctg
     2641 gaagcgttcc agcccgatgc cgcactcgta gcccgacgcg acccgtgcga cgtcgtcctt
     2701 gacgcgccgc agcgaatcga tccgcccctc ccagacgatc ttgccgtcgc gcagcaggcg
     2761 cgcctgcgca ttgcgcgata tctcgcccga aacgaccatg cagccggcga cggtgcccac
     2821 gcgcgacacg cggaagacct tgcggacctc cgcgcgcccg agcgtctgtt cctggtagcg
     2881 cttcttcagc tggccgacga gcgccgcctc cacatcctcc agcagttcat aaataatccg
     2941 gtagagcttc aggtcgatcc cctcgcgcgt ggccaggtcg ttggcccccg gctccggccg
     3001 gacgttgaac ccgatgatca cggcgtcgga ggccatcgcc aggttcacgt cgctctcgtt
     3061 gatgctgccg gtgccgctgt gcaggacgcg cacgcggacc tcttcgttgt gcagcttctc
     3121 cagggcctgg atgatggcct cgaccgaccc ctgaacgtcg gccttgagga tcacgttgaa
     3181 gtccttgatt tccccctcgg cgatgcggtc gtgcagccct tccagcgaga tgtgccgggt
     3241 ggtccccagc tcgacgagcc gccggcgctg ctcgcggcgc tcggcgattt cacgcgcctt
     3301 gcgctcgtcc tccatcacca gcagcgtttc gccgacctcg ggcgtgccgc tcaggccgat
     3361 cagctcgacc ggctgcgcca gcgtcgtggc ctgaaccggc cggccgcggt cgtcgtacat
     3421 cgcgcggacg cggccgtgct cctggccgac gacgaagtac tgcccgacgc gcagcgtgcc
     3481 gcgctggacc aggacggtgg cgactgagcc gcgcatcgga tcggtatgcg actcgatcac
     3541 gaccgcctgc gcgggccggt tcgggttggc cttgagttcg agcagctcgg cctggagctg
     3601 gatgatttcg agcaggtcgt ccatgccgcg gccggtcttg gccgaaatct cggcgaactc
     3661 cgtgtcgccc cccagttgcg tcgacagcac gctgtgctgc atcagctcct ggcgcacgcg
     3721 ctcggggttg gcctgcggca ggtccatctt gttgatcgcc acgatcagcg gcgtgccggc
     3781 cgccttcgcg tggttgatcg actcgatcgt ctgcggcatc acgccgtcat cggcggcgac
     3841 gaccagcacg acgatatcgg tgcacttgac gccgcgcgcg cgcatcgccg tgaacgcctc
     3901 gtggccgggc gtatccaaaa agacgatcgt cccgccgttg ggcgacttga tctggtaggc
     3961 gccgatgtgc tgcgtgatgc cgccgtactc cccttcggcg accgccgact tgcggtagta
     4021 atccagcacg ctggtcttgc cgtgatcgac gtggcccatg atcgtgacga ccggcgggcg
     4081 cggccgcagg tcctcggggc ggtcctcggc ctgcaggtac tcggcgacgt cggtctcatc
     4141 agcctcgagc tggatcttca gatcgacgtc gttatcgatc gccagctcct cggccagttc
     4201 aaagtccatc aactggttga tggtaatggc tttgcccttg aggaacgcct gcttgatgag
     4261 ctcggcgggg ggcagcccca ttttctcggc gaattccgag acggtgaccg gaccggtaag
     4321 cgtcaattcg gagactttct ggggaacctt gggttcgtcg cggcggcgat cgggcggcgt
     4381 tttggtccgt ttggcgcccg gcttgatcgg ccggcggaac tcgtcggcat cgccgtagac
     4441 acggctgggg cggaccccct tcttggcggc cgccgccttg gccttttcat cgcgctcggc
     4501 ctcgcccggt ttgcgacccc gtttcccccc gggcttgagc ttcatcacgc cgatctcggg
     4561 cagcgcggtc agttgctcgg gcgaaagcgg aatctccttg gcgtcgatcc ggcgtccgcc
     4621 gggccgccgc gcatcggcgc cggggcgctc gcctcccgcg gccgggcgtt cgcgccgcgc
     4681 gggcggcttc agaaacgggg aatcggcgct gaacttcggg gcggccggcg cggtcggctg
     4741 gccgatgcgg ctgacgccgg gcgccggccg gccggtttcc ggcgcggccg acacgcccgt
     4801 cgtccccgaa gagcttttca gctcggtggc caggcccgaa gggcgcagct tgcccggacg
     4861 cggcgcggcc gcggggcgcg tgcgttgttc gcccggcgcc gcgggggcgg cgctcggcgt
     4921 cggcgcttcg gccttgggcc ggggcggcgc gatcggcggc ggcggctcga ccactggcgc
     4981 cgcgggggct ttgccaatgg gctgaatcgg ggccggacgc cgacccggga tcggcacgac
     5041 tgggcgcggt ccgcccgccg gaccgggcgt gatcggtgcg ggccgcggcg gcgcggccgg
     5101 ccgacgcagc ggcggcggcg ccggggccgc tgggcgtgcc acgggcgcgg gcggagcggg
     5161 gcgctgtgcc ggcgccgacg gccgcccggg accggccgga cgcggtgcga ccactttcgg
     5221 cgcggccggt ttggcggcgg gagccgcggc cggcgtcgtc gccccctcgc gctggcgccg
     5281 ggcgtagatc tcgaacatca cgcgggcgaa gcgatcatcc accgaggacg cgtgggactt
     5341 gatcgtgtcg tggatctcgg cgaattccgg cagtgtgcgg aattcatcgg ccagatcccc
     5401 actcttgacg gtgatgttga gctgttggcc gatcgatttg gccaagtcat aaatccgcat
     5461 gcagctgtcg ctcctgcccc cgcgtccgga acgcgcaagg cttcagaatc caatcaaact
     5521 cactagataa ccgcatcgga cggttcaagt caagttgctc cggaataacg actcaaacca
     5581 ccgtgtcaac cagcgttgtg aatcggggat gtccgctgac cgatgtcgac accccccgcg
     5641 ggaggacgca ttgcacggct cggatttctg gtcctcgccg ctatcccgcc cgctgatcgg
     5701 ttacctcctg attctcggct tcggccggct cgccgctggc gctctcctga tcctggtcgc
     5761 cggcgccggt ttcggcttca ggctggcttt caccggcctc ttcggtctgg ccggactgct
     5821 ccgccccctc ggggttggcc gcttcggcgg cggcttcttc ctcgcgcagg cgccggcgct
     5881 cgacttccat ctcgcgcagc gcgttcagat agtcctcggc gcccacaatg agcgtctgga
     5941 ccagatcctc gttgccgccg gcgaatggca gcagcttggc cggatcggcc ttggcgatct
     6001 tttcgacgct gttgaagctg gagcgcagaa tcgcctgggc atacaactcg gaaagttcct
     6061 cgatctgatt gaggaagtcc tccagatagc ggcgctgaat ctcctcatac gccagctcgt
     6121 cctcgttctc cgaacggata tcgagccggt agccggtcag ccgcgccgcc agcttggtgt
     6181 tctggccttt gcggccgatc gcgatcgcca ggttgccctg ggcgacgacc acgtgcgcat
     6241 ggcgcgcctc gttgtccagc tcgatcgaga cgatcttggc cgggttgagc gcattgcgga
     6301 taaacgtgga cgcctccgaa ctccacggga cgatgtcgat cttctcattt tcaagctcgc
     6361 ggacgaccat ctgcacccgc gaacccttca tgccgacaca ggcgccaacc ggatcgacgt
     6421 cggaattgac cgatttgacc gcgatcttgg tgcggacgcc cgcctcgcgg gcgatcccga
     6481 cgatctgcac cgtcccgtcg gcgatctccg gcacctcctg gcggaacagc tcgatgacca
     6541 attggggaga cttgcgcgtc aggatgatgt tcggaccctt gggggtgtcc tcgacccgcg
     6601 cgatcagcac cttgagtcgg tcgttgaact tgtaacgggc gcccatcggc tgctcgctgg
     6661 cgggcaggac cccttcggcg cgaccgatgt tcaggatcac gtcgcgccgc tcgaagcgct
     6721 ggacgatccc ggtcaccatc atgcgctcct tatccttgaa ttcctcaaag atcttggcgc
     6781 gttcggcgtc gcgcagccgc tgcatcacca tctggcggac cgactgggcg gcgatgcgcc
     6841 cgaactcgct cggctcgatc ggcacgcgca cgtcctggcc gaccttcagc ttgcggtcgc
     6901 cggtcagctt gcgggcttcc ttcaggccga tctcgcgcgg atgacccttg cgcccttcct
     6961 cgacgatcgt cttggtcacg atcacgttga gcgtgccgtt ttccaggtcc agattggcca
     7021 ccgcgtcgcg atagttgagc aactgcttgc gtgaggccga cagaatggcg tgctcgatcg
     7081 cctccttcag gatttcgggc gggagatcct tctccttgct gatcagccgg acaaaggctt
     7141 tgaggctgtt cgagtccatg accatgatcg tcgtgctccg cggcgggtcg cccgggggca
     7201 accggcctgc cataatgggc gagacggcgc cggaccgggc cgtggaccgg gcggcgtcgc
     7261 catctctcgt ggagccctta caacaaaaaa gtgggcaccg ctgcccaccc gcaaactcca
     7321 caaggctcaa ccccggtact atagtcaagg gcccggtccg ttgcaagcgg gaaaagcccc
     7381 ctggaacggg ccgggcataa aaaagcgcaa aaacggcgct ttcgcgggcc gatccgcagg
     7441 agcttcggcg gcacgcgttg cccatcatcc ggcggttcac aacgagggct gataatggag
     7501 ggtgcacggc cgattcgaac ggcccaggcc ttgccgggtc cttcagcagg ccagtccccc
     7561 gtcagggacg gcagccttca gcccgcagct tcagtgcttg tcctttccct tatcgcaatc
     7621 cgcccaagga ttccaaatcc aatccggacg attccgactc gattgccggc tgagtccgcg
     7681 aagcggacga ccggagtaaa gcgcagggtg acggccgcag gccggaaccc tgcatcgccg
     7741 cccccacgat tcaagcccct gaaaggggcg gccggagccg gtctgaattc ggggaatgac
     7801 aaattcttgc ccggcgggaa ccgggatacc ccctgaaaac atgaaactcc tcgcgtctcc
     7861 tatctccctc atgtccttta cgtcccagac gtcccttctg aaaaacggat cccgcccgag
     7921 gcacgcccca ccacggatga gcgacagatt tgcattttca acacagaatc ccgaagatag
     7981 gagggtccgc gccgatttga cgccggacgc attcctgaaa cggggaccgg acgatggcat
     8041 tcaacgaggc acgggtatcg cgaggcttca gcctggcgtg ctggggcctg gccctgatcc
     8101 tgttcgatct tccctggcgg cgctgggtca tcccgcctga tttcttcggc tggatcgtcc
     8161 tgacgatcgg gctgttctgg ctgcaaccat tgggcgcgcg cattcagaaa gccatgtgga
     8221 tcgccggcgc gatgatcgcc ctgtcgctcc ccgagctcct cggctatcgt cgcgaactcc
     8281 tctttcacct cgggcggccg tattacatcg acctgctctt tcccctgccg cagctcctgc
     8341 tgatcgccgt cgtcctgctc ctgctccatg cgatcatcga tcgcgccaac agcctggagc
     8401 atgtggagct gagccatgac accggcgtgc ggcgcaacct cttcctcagt tggttcgtgg
     8461 tctacacgct ggcgctgtgg accagcctgt tcatgccctc gctgcaccgc tacctgatgg
     8521 cgattccgtg gctctacatc atcgtgatcc tggtgctgct gatcctgctg ctgcgccggg
     8581 cgagcgagga actggtccag gccgaggcga ccgccagcgc ccggccgagc cgctagcccg
     8641 accccccccg ctccgcgacc gaggacgcgc acgccccgtg ttgagctttc tgctacgccg
     8701 tctggcccag tcgctcgccg tcctgctggc gatctcgatc ctggttttcg tcatgatgta
     8761 ctgcctgggc gacccggtgg ccacgctgct gccgcaaaac gcctcgcagc gtcagcgggc
     8821 cgaattgcgc gcggcgctcg ggctcgaccg cccgctgccg gtgcaatacc tgatttacgt
     8881 cggcaacctg ctgcgcggcg atttcggcct gtcgtatcat cagggccgcc ccgttggcga
     8941 gatgctcgcc gagcgagccc ccgccacgct ggaactggcg tgcgtcgccg tcctgttttc
     9001 cgtcctcatc ggcgtgccgc tgggggtcct ggccggcgcc cgcgcgcgcc atccgctgtc
     9061 gcgcctggtg ctggggggct cgctgctggg gatctcggtc ccttcattct ggctcgggct
     9121 gctgctgatg atgctcttcg gcgtcctgct cggctggctg ccgccgatgg gacgcggccg
     9181 ca
//
LOCUS       DAOPCO010000158         9069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7221_length_9069_cov_5.012425, whole genome shotgun sequence.
ACCESSION   DAOPCO010000158 DAOPCO010000000
VERSION     DAOPCO010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9069
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7221_length_9069_cov_5.012425"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLS90_11180"
     CDS             <1..901
                     /locus_tag="PLS90_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK03008.1"
                     /translation="YADIAPHDYNIDADQLESLVTERTRAIVIVHFGGQLCDMEQICA
                     VARKYGLRVIEDAAHAHGVMDHAGRCAGAFGDIGCFSFQAAKNLATGEGGMVITNDKE
                     LYDRCFSIHHYGRRPGELWYKHFMTGWNYRMNEFTAAVGIPALQRLHQQNELRQANCD
                     YLTAGLARIPCLTPITDTRRQRRHSRHLVIIRYDRAKNDGVHRDDLIKALVAEGIPAM
                     AGYTFPNYANPLFYDQETKDRFKAAGIDFPDYSVYRERCPNVERACNEEAIWLEHRLL
                     LGTREDMDDIITAFTKVTAALGG"
     gene            995..1909
                     /locus_tag="PLS90_11185"
     CDS             995..1909
                     /locus_tag="PLS90_11185"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK03009.1"
                     /translation="MELLVKLGDVELEYGGPLLGELRESDPRSSDATLRERLENDGYL
                     LIRGLHPRDQIERARLTILEYLAGYGVLDPDAPLTEGRINLAQGPSGGFQPDMRAMSR
                     YFSARDDINDIVLAPERMNLIERLLGGPALSFDYRWMRIMGHAASTPAHCDIVYMGLG
                     ARRLLSVWTPFMDVPLDQGGLALCAGSHRWTRLRETYGAMDHYRDRTEALFDSLPLEL
                     SERWGGHWLTTHFRMGDVLVFDAYLLHASLENQTDRFRLSLDSRYQLRADPVDERWMG
                     ENPYSLEARRADDQQKRPLCEARREWGI"
     gene            1912..2769
                     /locus_tag="PLS90_11190"
     CDS             1912..2769
                     /locus_tag="PLS90_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK03010.1"
                     /translation="MKKSLSYWSFPGGLSGEMPIIEAARSAREAGYQGIELALSWRGE
                     LTAETTDKEVARLRDALRNEGMQIAGLASVLTWDRLLTAEDAHERQAAIDLVARGLRV
                     GAILEAGAFLIIPGAVDVFFRPDLSSISYSDADERLRRALDALVPIAEECGVALALEN
                     VWNRFLLSPLEVRTLVDAYRSEFLGVYFDVGNVMLTGYPEDWIRILGHRIRRVHFKDF
                     KRSVGTAEGFVDLLAGDVNWPAVMRALRQIGYDGFCTAELIPCYRFAPEVRWWNASRA
                     MDAIFAMAG"
     gene            complement(2819..3466)
                     /locus_tag="PLS90_11195"
     CDS             complement(2819..3466)
                     /locus_tag="PLS90_11195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPK03011.1"
                     /translation="MLVEAGSRILFTGDSITDAGRARPVGEGAGLGDGYVALMDALFT
                     AVCPERRLHVLNTGIGGNTSRDLLARWQSDVVDLGPDWLCVMIGVNDAFYQQAEPPQP
                     ERSVDEAEFEANLRRAIEPVRPGLRGLVLMTPFYLERDRKDRLRAAVDRYGEIVLRLA
                     REFDAVGVDTQAAFDAVMAVTPEVRLADDKVHPNRRGQMIIARAIAGALRLPPGS"
     gene            complement(3517..4647)
                     /gene="mqnE"
                     /locus_tag="PLS90_11200"
     CDS             complement(3517..4647)
                     /gene="mqnE"
                     /locus_tag="PLS90_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HPK03012.1"
                     /translation="MASVSETPAARPIGGFHDPFLETVARRLEAGERLTREDGLRLYE
                     SPDLLGVAWLANRERERRHGLKTYYNINRHINYSNICVNHCKFCAFARDPGADGAWEY
                     QLQEIFDKAERDMPPGGDELHIVGGLHPSLPFEYYTEMLRGLKARLPHVHLKAFTAIE
                     IAHLARISGLKVQQVLERLIEAGLGSLPGGGAEVLTANSRKLVCGEKLTGKGWLNIHR
                     IAHQMGLKTNSTMLYGHVESIADRVDHVLLLRELQDETGGFQVFIPLAFHPKFSHMQD
                     LPAPSGATDLRVMAVSRLLLDNIDHLKAYWIMLGVKTAQVAQHFGANDLDGTVVEETI
                     YHMAGAETPQSMSEKELRRLIRETGRVPVRRNTLYEELEAVG"
     gene            5185..6210
                     /locus_tag="PLS90_11205"
     CDS             5185..6210
                     /locus_tag="PLS90_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK03013.1"
                     /translation="MRNYIIRRLMLMVPTFLGITFVTFVLTQFVPGGPIDQLRLQMIG
                     AGGEGGSTGGARDRVQFVIPEDQLAILKRFYGFDKPIPVRYLVWLGRIVRLDFGASFR
                     YTEPVIDTISQRLPVSIYYGLWTTFFTYAISIPLGIVKAIRHRSLIDNASSVLIFVGY
                     AIPGYALGAVLLVLFAVNRSWFPLGGFESAGFDSLPFWGKVWDRLHHSILPLICYLIG
                     SFAVTTMLMKNSLMENMSADYVKTALAKGLTWRRAIFVHAVRNSLIPLATSIGHLIGL
                     ILTGSLLIERVFNIRGMGLLYFEAIQARDYPVVMGIIVISGVLLLVGNLLSDLCVAAV
                     DPRVRFE"
     gene            6216..7298
                     /locus_tag="PLS90_11210"
     CDS             6216..7298
                     /locus_tag="PLS90_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPK03014.1"
                     /translation="MHLPRLDPITRKRFQRFRRIRHGYWSLIILAVLTLLSLFAEYLA
                     NDRAILVRYNGRFYWPTWRFIPMETFGQEDEWGFSDAETDYLALRDSWRAAGADNWLI
                     MPPIPWDPFENDFWYTEQRGEFPPNPPDFARGHYLGTDSQGRDVAARLLYGFRISMLF
                     ALALVLAGQALGTVIGSLQGYLGGVFDISSQRFIEIWSSLPTLYIIIIMATFFTPSFW
                     MLLGIMVLFEWIHITYFMRTEMYREKAREYCLAARSYGASHARIIFRHLLPNCLTPLV
                     TFTPFAMVGAIFALTSLDFLGYGLPPPTPSWGELLDQSLQVENRGKLWMVLSPFAALT
                     ITLILVTFVGEAVREAFDPKQYARYE"
     gene            7366..>9069
                     /locus_tag="PLS90_11215"
     CDS             7366..>9069
                     /locus_tag="PLS90_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPK03015.1"
                     /translation="MNRFLLGCLAAWLIPTSLALAQPAPTADGATTAPLAPPPGEIPW
                     VTNLDDPPIGSLEAIKGGTFNTFMYDYPLTFRLMGPNSNDAFAAWNRAYTMSFTLLQR
                     HPVTDNYIPWMASHWAVMPDNRTVYYKLRPEARWTDGEPVTADDYVFAWEMMGSEAIV
                     DPFYNEYVATYFESVEALDDLTLKVVGKQPSWRPLDDYALFPMPRHAIDLGPDWVQRA
                     NNTFQVAAGPYRVTEAITGKHVVFDKVPGWWGADLDYFRGLYNVDRIVVHVIPDGREL
                     DYFKQQQIDMTLVASARIWAEETDFPAIQNGWAHKKRVFVDTPQGIYGLHMNLQFPLF
                     ANKDFRKAMQYLFDFDTLNKNLMYDAYFRIASAFEGTPYANPELKPYPFDPRKAREHL
                     VKAGFTQRGPDGILVDDQGRRASFTVIFGGETIERHLTVLREIYKRAGVEMNLQLMDG
                     ATAFNRGLERKYEMTLTGRTAGFYPEPKQYFHSTFVESTNNNNIWGFGSPETDQLIET
                     YMFDMDLQHRLDAMHRLDAIVHDEAFYIPFWTAPYLRLLYWDYVVWPEFILPKRTESS
                     TD"
BASE COUNT         1502 a         3346 c         2559 g         1662 t
ORIGIN      
        1 ctacgccgat atcgcccccc acgattacaa tatcgacgcc gatcagctcg aatccctcgt
       61 taccgaaaga accagggcaa tcgtgatcgt tcatttcggc ggtcagctct gcgacatgga
      121 gcaaatctgc gccgtcgcgc ggaaatacgg cctgcgcgtc atcgaggacg ccgcccatgc
      181 tcatggcgtc atggatcacg ccggccggtg cgccggcgcc ttcggcgaca tcggctgctt
      241 cagcttccag gccgcgaaaa acctcgccac cggcgagggc ggcatggtca ttaccaatga
      301 caaggaattg tatgatcgat gcttctccat ccatcattat ggccgccgtc ccggtgaatt
      361 gtggtacaag cacttcatga ccggctggaa ttaccggatg aatgagttca ccgcggcggt
      421 cggcatcccc gcgctgcaac gcctccatca gcagaatgag ctccgccagg cgaactgcga
      481 ttacctgacc gccggcctcg cccgcattcc ctgcctgaca ccgatcaccg acacccggcg
      541 ccagcggcgc cactcccgtc atctggtcat tatccgctac gaccgtgcca agaatgacgg
      601 cgtgcatcgg gacgacctga tcaaggcgct cgtcgccgag ggcatccccg cgatggccgg
      661 ctatactttt ccgaattatg ccaatcccct cttctacgat caggagacta aggatcgctt
      721 caaggccgcg ggcatcgact ttccggatta ttccgtctat cgtgaacggt gccccaacgt
      781 cgaacgcgcc tgcaacgagg aagcgatctg gctcgagcac cgcctgctgc tggggacacg
      841 cgaggacatg gacgacatca tcacggcctt caccaaggtg accgccgcgc tgggcggctg
      901 atgatgagct cgaacacatt ggaattgctg ggccttgaac caccggttcg aacggccgag
      961 gtctcgccga attccgatcc gcgggaggct ccgggtggaa ctgctcgtaa aactgggtga
     1021 cgtcgaattg gaatatggcg gccccctgct cggggaattg cgcgaatcgg accctcgttc
     1081 aagcgacgcg accttgcgcg agcgactgga aaacgacggc tatcttctga ttcgcggcct
     1141 ccatccgcgc gaccagatcg agcgggctcg cctcacgatc ctggaatatc ttgccggcta
     1201 cggcgtcctc gatcccgatg ccccgcttac cgagggcagg atcaatctgg cgcaaggtcc
     1261 atccggcggc ttccagcccg acatgcgcgc catgagccgc tatttttcgg cacgggacga
     1321 catcaatgac attgtcctgg cgcccgaacg catgaacttg attgaacgtc tgcttggcgg
     1381 cccggcgctc tccttcgatt accgctggat gcgcatcatg ggtcacgccg cttccacgcc
     1441 cgcgcactgc gacatcgtct acatgggcct cggcgcccgc cgcctcctct ccgtctggac
     1501 ccccttcatg gatgtccccc tggatcaggg tggcttggcc ctctgcgccg gatcgcaccg
     1561 ctggacccgc ctgcgcgaga cctatggcgc gatggaccat taccgggacc ggactgaggc
     1621 gctctttgat tcgctcccgc tcgaactgag cgagcggtgg ggcgggcatt ggctcaccac
     1681 ccatttccgg atgggggacg tcctggtttt tgacgcctac ttgctgcatg cctcgctgga
     1741 aaaccaaacc gaccggttcc gcctgagcct cgactcgcgc taccagcttc gcgccgatcc
     1801 cgtggatgaa cgctggatgg gcgaaaaccc ctattcgctc gaggcgcggc gcgccgacga
     1861 ccaacagaaa cggccgctct gcgaagcgcg ccgggagtgg ggaatctgat cgtgaagaaa
     1921 agtctgagct actggtcctt tcccggcggc ctctccggcg agatgccgat catcgaggcc
     1981 gcccgctcgg cccgcgaggc cggctaccag ggcattgagc tcgccctcag ttggcggggc
     2041 gagctgacgg ccgaaacgac cgacaaggag gtggcccgcc tccgcgatgc cctccgcaat
     2101 gaaggcatgc agatcgccgg actcgcctcg gtgctgacct gggaccggct gttgacggcg
     2161 gaagacgccc acgagcgcca ggccgcgatc gatctcgtcg cccgcggcct gcgggtcggc
     2221 gccatccttg aggccggcgc cttcctgatc atccccggcg cggtcgatgt ctttttccgt
     2281 cccgatctgt ccagcatctc ctacagcgac gccgatgaac gcttgcgccg cgcgctcgat
     2341 gcactggtcc cgatcgccga ggaatgcggc gtcgccctgg cgctcgaaaa tgtctggaac
     2401 cggtttctgc tcagtccgct cgaagtccgc accctcgtcg atgcctaccg gagcgaattc
     2461 ctcggcgtct atttcgacgt cggcaacgtg atgctgaccg gctaccccga ggattggatc
     2521 cggattctcg gccatcgcat ccggcgcgtc catttcaagg acttcaagcg ctcggtcggc
     2581 accgcggagg gcttcgtcga cctgctcgcc ggcgacgtca actggccggc cgtgatgcgc
     2641 gccctgcgcc agatcggcta cgacggcttc tgcaccgcgg aactcatccc ctgttatcgc
     2701 tttgcccccg aggtgcgctg gtggaatgcc tcgcgggcga tggatgccat cttcgccatg
     2761 gccggctgag cggcggcgcg gccgcggtca tcgcggcgtc ggatccccgc ggccgcaatc
     2821 aggaccccgg cggcaggcgc agtgcgcctg caatcgcccg cgcaatgatc atctggcccc
     2881 gccggttggg atgaaccttg tcgtccgcga gccggacctc cggcgtgacg gccatcaccg
     2941 cgtcgaacgc ggcctgtgta tccacgccca cggcgtcaaa ttcgcgcgcc agacgcagca
     3001 cgatctcgcc ataccgatcc accgccgccc gcagccggtc cttccggtcg cgctccagat
     3061 aaaacggcgt catcaggacc agtccccgca gccccggccg cactggttcg atggcgcggc
     3121 gcagattggc ttcgaactcc gcttcatcga cggagcgctc cggttgcggc ggctccgcct
     3181 gctgataaaa ggcgtcgttc acgccgatca tcacgcacag ccagtccggc cccagatcga
     3241 ccacatcgct ttgccatctc gccagcaggt cgcgcgacgt gttgccgccg attcccgtgt
     3301 tgagcacatg cagccgccgt tcggggcaga ccgccgtgaa caatgcgtcc atcagcgcca
     3361 cgtagccgtc ccccagcccc gcgccctcgc ccaccggccg cgcccggccg gcgtcggtga
     3421 tcgaatctcc cgtgaacagg atgcggctcc cggcttcgac cagcatgctc gcgtgtcctt
     3481 tcgagtgggc ggcgcccgcc gccggccgga gcggtctcag ccgaccgctt ccagttcctc
     3541 atacagcgtg ttgcgccgca cgggcacgcg ccccgtttcg cggatcagcc ggcgcagttc
     3601 cttttcgctc atcgattgcg gcgtttcggc cccggccatg tggtagatcg tctcctccac
     3661 caccgtcccg tccagatcgt tcgcgccgaa atgctgcgcc acctgcgccg tcttgacccc
     3721 cagcatgatc cagtacgcct tgaggtggtc gatgttgtcg agcagcagcc ggctgacggc
     3781 catcacgcgc aggtcagtcg ccccgctcgg cgccggcagg tcctgcatat ggctgaattt
     3841 ggggtggaac gccagcggga tgaaaacctg aaaccccccg gtttcgtcct gcagttcgcg
     3901 cagcagcagc acgtgatcga cgcggtccgc gatcgactcg acatgcccat acagcatcgt
     3961 gctgttggtc ttgagtccca tctggtgggc gatgcggtgg atattgagcc agcccttgcc
     4021 ggtcagcttc tcgccgcaga ccagcttgcg gctgttcgcc gtcagcacct cggcgccgcc
     4081 gcccggcagg ctgcccagcc ccgcctcgat cagccgctcc agcacctgct gcaccttgag
     4141 ccccgaaatc cgcgccaggt gcgcgatctc gatcgccgtg aacgccttca ggtggacatg
     4201 cggcaggcgc gccttcaaac cccgcagcat ttcggtgtaa tactcgaagg gcagcgacgg
     4261 gtgcagcccg ccgacgatgt gcagctcatc cccgcccggc ggcatgtcgc gctccgcctt
     4321 gtcgaagatc tcctggagct gatattccca cgccccgtcg gccccggggt cgcgcgcaaa
     4381 ggcgcagaac ttgcagtggt tgacgcagat attcgagtag ttgatgtgcc ggttgatgtt
     4441 gtaataggtc ttcagcccgt ggcgccgctc gcgctcccgg ttcgccagcc acgccacccc
     4501 cagcaggtcg ggcgattcat agaggcggag gccgtcctcg cgcgtcagcc gctcgcccgc
     4561 ctccagccgc cgcgccaccg tctccagaaa gggatcgtgg aacccgccga tcggcctcgc
     4621 cgccggcgtc tcgctgacac tcgccatcgg cctgcatctg tcctttcgtg gcgcccttcg
     4681 cgcacgcccg ttcgactcta ccaccccgcg cccgcccgcg caacgccggc tccgcatgac
     4741 tcaatttgag ccttcaaatt cttttcggat ttcgcaatgt cgcatttcct gctcgcggcc
     4801 gaccggccgc ctgagtaact ccgcgaagcg gacggtcgga ataaagcaca gggtgaaggc
     4861 cgcaggccga atccctctgt cgccgcgtcg gaccgatcaa cccctgaaac gggtggccgg
     4921 agcacctggc gctggccggc gaggctcaca ctccatctca ggaagtcacc gtcacagctc
     4981 ttggaacgca tgttctcgtg catgctgctc ggtttcccca tccaacccgc caagccgatg
     5041 tccgcctcac cccttcggca tccgaccctc gcctgcctcg ctcaccccct cccaccggcc
     5101 ttgtgcccgc cgacggctcg gcggtacaat acccgaccca gtcccgtccg gggagacccc
     5161 atggccacca gccgccgcca cggcctgcgc aactacatca tccgccgcct gatgctgatg
     5221 gtcccgacgt tcctcggcat caccttcgtc accttcgtgc tgacgcagtt cgttccgggc
     5281 ggcccgatcg atcagctccg cctgcagatg atcggcgccg gcggcgaggg gggctccacc
     5341 ggcggcgccc gcgaccgcgt ccagttcgtc atccccgagg atcagctcgc catcctgaag
     5401 cgcttctacg gcttcgacaa accgatcccc gtccgctatc tcgtctggct cggccgcatc
     5461 gtccgcctcg atttcggcgc ctcgttccgc tataccgagc cggtcatcga cacgatcagc
     5521 cagcgcctgc ccgtctcgat ctactacggc ctgtggacca ccttcttcac ctacgccatc
     5581 agcatcccgc tgggcatcgt caaggccatc cgccaccgct cgctcatcga caacgcctcc
     5641 tccgtcctga tcttcgtcgg ctatgccatc cccggctatg ccctgggcgc cgtgctgctg
     5701 gtgctcttcg ccgtcaaccg ttcctggttt ccgctgggcg gcttcgaaag cgcgggcttc
     5761 gacagcctgc cgttctgggg caaggtctgg gaccgcctcc atcattccat cctgcccctg
     5821 atctgctacc tgatcggctc gttcgccgtc accacgatgc tgatgaagaa cagcctgatg
     5881 gagaacatga gcgcggacta cgtcaagacc gccctggcca aggggctgac ctggcggcgc
     5941 gcgatcttcg tccacgccgt ccgcaattcc ctgatcccgc tcgccacgag catcggccac
     6001 ctgatcggcc tgatcctgac cggctcgctg ctcatcgaac gcgtgttcaa catccgcggg
     6061 atgggcctgc tctatttcga ggcgatccag gcccgcgatt accccgtcgt catgggcatc
     6121 atcgtcatct ccggcgtcct gttgctggtc ggcaatctgc tctcggatct ctgcgtcgcg
     6181 gcggtcgacc cccgcgtccg ctttgagtga ccgcgatgca cctgccgcgg ctcgacccca
     6241 tcacccgcaa acggttccag cgcttccgcc gcatccgcca cggctactgg tcgctgatca
     6301 tcctggcggt cttgacgctg ctctcgctct tcgccgagta tctcgccaac gaccgcgcga
     6361 tccttgtccg ctacaatggc cggttctact ggccaacctg gcggttcatc ccgatggaga
     6421 ccttcgggca ggaggatgaa tggggcttca gcgacgccga gaccgactat ctggccctgc
     6481 gcgatagctg gcgcgccgcc ggcgccgaca actggctgat catgcccccc atcccctggg
     6541 accccttcga aaacgatttc tggtataccg agcagcgcgg tgaattcccg cccaatccgc
     6601 ccgacttcgc ccgcggccac tatctgggca ccgactccca gggccgcgac gtcgccgccc
     6661 gcctcctcta cggcttccgc atctcgatgc tgttcgccct ggcgctcgtc ctcgccggcc
     6721 aggccctcgg caccgttatc ggcagcctcc agggctatct gggcggcgtg ttcgacatct
     6781 cgtcgcaacg cttcatcgag atctggtcct ccctgcccac cctctacatc atcatcatca
     6841 tggccacttt cttcacgccc agcttctgga tgctgctggg catcatggtc ttgttcgagt
     6901 ggatccacat cacctacttc atgcggaccg agatgtaccg cgagaaggcc cgcgaatact
     6961 gcctggcggc gcgctcctac ggggcgtccc atgcgcgcat catcttccgc cacctgctgc
     7021 ccaactgcct gaccccgctg gtcacattca cgccgttcgc gatggtcggc gcgattttcg
     7081 ccctcaccag cctcgatttc ctcggctacg gcctcccccc gcccaccccg agctggggcg
     7141 agttgctcga tcagtccctc caggtcgaga atcgtggtaa actttggatg gtcctctccc
     7201 cgttcgccgc gctgacgatc acgctcatcc tcgtgacatt cgtcggcgag gcggtgcgcg
     7261 aggccttcga tcccaaacaa tacgcgcggt acgagtaggc ggcgcgcgcc aacgcccgga
     7321 cgccttaccc gacccgccga acaccgaaca gacaaggacc cgaccatgaa ccgcttcctc
     7381 ctgggttgcc ttgccgcatg gctgatcccg acctcgctgg cactggccca gccggccccg
     7441 accgccgacg gcgcgaccac cgcccccctc gcgccgccgc ccggcgagat cccctgggtc
     7501 accaacctcg acgaccctcc catcggctcg ctcgaagcca tcaagggcgg cacgttcaac
     7561 acgttcatgt acgactaccc gctcaccttc cggctgatgg gccccaactc caacgacgcc
     7621 ttcgccgcgt ggaaccgcgc ctacacgatg agcttcaccc tcctccagcg ccaccccgtc
     7681 accgacaact acattccctg gatggccagc cactgggccg tcatgcccga caaccgcacg
     7741 gtctattaca agctgcggcc cgaggcccgc tggaccgacg gcgaaccggt caccgccgat
     7801 gattacgtct tcgcctggga aatgatgggc agcgaggcga tcgtcgaccc cttctacaac
     7861 gaatacgtgg cgacctactt tgaatcggtc gaggccctcg acgacctgac gctcaaggtc
     7921 gtcggcaagc agcccagctg gcgccccctc gatgactacg ccttgttccc gatgccgcgc
     7981 cacgcgatcg acctggggcc cgactgggtc cagcgcgcca acaacacgtt tcaggtcgcc
     8041 gccggcccct accgcgtcac cgaagccatc accggcaagc acgtcgtctt cgacaaggtc
     8101 ccgggctggt ggggcgccga tctggattac ttccgcggcc tctacaacgt cgaccggatc
     8161 gtcgtccacg tcatccccga cggccgtgag ctcgattact tcaaacagca gcagatcgac
     8221 atgaccctcg tcgcctcggc gcgcatctgg gccgaggaga ccgatttccc cgccatccag
     8281 aacggctggg cccacaagaa gcgcgtcttc gtcgacacgc cgcagggcat ctatggcctc
     8341 cacatgaacc tgcagttccc cctcttcgcc aacaaggact tccgcaaggc gatgcaatac
     8401 ctgttcgact tcgacaccct gaacaagaac ctgatgtacg acgcctactt ccgcatcgcc
     8461 tcggccttcg agggcacccc ctacgccaac cccgagctca agccctatcc gtttgacccg
     8521 cgcaaggcgc gcgagcatct ggtcaaggcc ggcttcaccc aacgcggacc cgacggcatc
     8581 ctcgtggacg accagggccg ccgtgccagc ttcaccgtga ttttcggcgg cgaaaccatc
     8641 gagcggcatc tgaccgtcct gcgcgagatc tacaagcgcg ccggcgtcga aatgaacctc
     8701 cagctcatgg acggcgccac cgccttcaac cgcggcctgg agcgcaaata cgaaatgacc
     8761 ctcaccggcc gcaccgccgg cttctacccc gagcccaagc agtacttcca cagcaccttt
     8821 gtcgagtcga ccaacaacaa taacatctgg ggttttggct cgcccgaaac cgaccagctg
     8881 atcgaaacct acatgttcga catggatctg cagcaccgcc tcgacgcgat gcaccggctc
     8941 gatgcgatcg tccacgacga ggcgttctac atccccttct ggaccgcccc ctacctgcgc
     9001 ctgctctact gggactatgt cgtctggccc gaattcatcc tgcccaagcg caccgagagc
     9061 tccaccgac
//
LOCUS       DAOPCO010000159         8993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7286_length_8993_cov_5.992056, whole genome shotgun sequence.
ACCESSION   DAOPCO010000159 DAOPCO010000000
VERSION     DAOPCO010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7286_length_8993_cov_5.992056"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3646
                     /locus_tag="PLS90_11220"
     CDS             <1..3646
                     /locus_tag="PLS90_11220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03016.1"
                     /translation="ARHDLAEISRQRAELHDRLEETRLALEDARREAAAQGEGRAQAV
                     AAVRRELEDHLAELGQLTSKQEVQLARLRKELGDLRKESNALLLEKRHGEDVISKLIH
                     SLTYCQNKAWEQSTWVDEQLRSRGYVLGLYFEAVRRNKARALLTAVRWVARRLLGRGF
                     SYARAMGVEYRLHTIKGELQALSFYARDAEHQVSDLAIASPTSMAGGLLPSAQRPEPD
                     LIVYSITNWDFRMQRPQHIVSRLVQAGSRAFVMRDTTIGLEEEFPSDQTIDNHMLVRP
                     LGRDLWEVQPCTYHPLNIYKDNLADPRDMALLKRSLGLLRERFSIGDHVALVQLPFWG
                     PLAFALTGARVIYDCMDNHSDFSTNDPAMLQQEEHLLTSADAVVVSSAYLDELTGSKP
                     RVKYVVRNACEFDYFSREPDSLDPLVREAEQPVIGYYGAISDWFDVDLVESVARRRPD
                     WRFILIGYTFGADITRLQALPNVSFTGEIPYSRLTSFLYGFDVCLIPFRLTELIRATN
                     PVKIYEYSAAGKPTVATPIPEVVALGDLVRTAADARGFEQAIADALAETDKPARALRL
                     REFAQHNTWDARVEDFKRIIRRHAFPKVSIVILSYNNLRYTLDCLESILTQSSYPNFE
                     VILIDNASPELEVREALGKLEDPRLRIVLNEENKGYAGGNNQGMAMARGDIIILLNND
                     TICPADWIERLVEPLRDNPRLGMVGPMTNSAGNEQMLDVFCAGASSQHTWLRDFYRVR
                     RGRRHLTDRLGFFCVALRRELYEQIGDLDEGFELGYFEDDDYCLRAVAAGWQLAVIED
                     AFVYHHGSVSFNSIDTERRRVLQERNRRRFEKKHQRRWALPICEILYYGAEGPAHEPD
                     RPTALIGPCTDWSGPLGRPQQIAAALVHAGFRVFYVTNNLRHDRFLGIRMLCPFLYIV
                     ENRDQWLTDIQPQPLDLFISFGPRGDSITTKVRARSYLMDFDAYPFRESWKWNPSDPA
                     ALAVAAKADWITVSQDFLTGELLGVPHQRVHLLPNGVVAAHWSTNKPLTPPEEIREIL
                     RKQGGPLIAILGVLNDGSVNLNWLREVADARPEWSFLFILADESTGDDDEHDFFRLRN
                     VHLLGWKPVATLPDYLHFADVGLLPPATLADNRIYPRALLLRLIGAGLPVVSGYPVHE
                     PQFTGIVTHAPRPEDAVLAIRQAMGKAHLEPFSRRCAELVAAEDWSVLIRSPLAAIEP
                     VLKERRSRPA"
     gene            3708..5951
                     /locus_tag="PLS90_11225"
     CDS             3708..5951
                     /locus_tag="PLS90_11225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03017.1"
                     /translation="MAGWHLLTRLQANWRRYLRRRDHGRPASLAALRAWAVPDRVSIV
                     LPTYNGRRHLDEAIESIRAQTWPHWELILVDDGSTDGTGDLADQWARADPRIRVIHQA
                     NQRLPGALNTGFRAARGEFHTWTSDDNRLRPGFLAALVAVLRERPAADAVYTDYDLID
                     EAGRPLLDSPVCRALQSPPGAARLRLPRQPFDLNFVNGNYLGGAFLYRAHATRLLWGR
                     EWDGPYSPNRFLCEDYDFFLRLNAWLRLEHSGLEQSLYEYRLHSDSLTATARQGRVRE
                     LSEELLRFDDFRRDMTLWPILWHIESVDDPTRLGSRLRLRALDVGDHVTAEREAPPWL
                     AAVWVRVDNSETQPGCDVPAGWARVLVCRRPPRGDLSDWDVILTEAKPGDDPPPGAPI
                     LRYRDEEALWHALRVAAWQRQAVAAEARAFDETPPRHRLSVVVGTNRPSLRLRPLIDS
                     LADQTAPKSIWELLLVDNARGRHPRELTSGLPRLAELPHVRSLSNPVPGLSITRNAGL
                     AEARGAIVLFLDDDVTLAPTALAALLAAWAAVPDAGVMGGAIELAPLDRPPRWLGPAV
                     LKYWGGLAAGLPARHPAATWRELPFGGFWSARAALLRRIGGFRGRYGRAGLATDAGEE
                     IVAALQLRADGRASWLEPAARALHHVSRDRITLPAFFRIRKTNADTFFLLSSEGRIDD
                     DPGLSASLRSGLRQILRALAPVPRPLDVRLDHLGNAVEHLWLAHRLFYYRLGRLRPGS
                     PPAAPRQ"
     gene            6135..>8993
                     /locus_tag="PLS90_11230"
     CDS             6135..>8993
                     /locus_tag="PLS90_11230"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPK03018.1"
                     /translation="MRCNALRHAIRRGLTRGWITSMLLALALGFTPGPDLSAHDDDRA
                     EIRGHVTDAVTGKPIEGANVFAVRDKSNSSLQRKTDGKGKYEFKHLEPGPWVIEVGKP
                     GYAPQRAEVVLQPDKRLELDFALQPNMDDVGEIAGAVIDNETGEPVPDAFVFVRDPLK
                     DGPHDVIGDSTDEHGRYSIPGLPAGRYEVHAVHPDYERAKKTVEVHAGLLSVLNFDLT
                     PRQEPGTGSLLVRVADADTGLPVAEAIVTIEGIFTFAPLLTDENGELAFDSLAAGERR
                     VQVDKQGYEHQQQIARIEAGRQTLLEFELVPLSGSRLGAIAGRVINGANEAPIEHARV
                     YYRLIEHDDDDDDKSHDASPDGPFVETGPDGVYEIPDLRPGLYGLQVVAEGFYSQWNK
                     AEVKPGETTRVPFALRLIVPAEAGFLAGQVTDAATSEPVVGARVFFARQDEVDKTMML
                     DATDESRPHVVTDENGNYETPPLRPGQYLLVVRARGYEPARRMAEVRSGETTEVNFAL
                     APFVPVEPGSITGLVLDGLTSQPIAGALIFFAPVVPDAPDGSVAPHDDLADHLPSVRT
                     DNTGRYQIDDLRPGRYRLIARARGYLPATARAEVQSGQSTRVNFALRPDPNPDAGVIQ
                     GRVIDRESNLPLAGAFVFFARLPINTVEPPAALNEFGAMMSIEPPAGGDDYVRTNGRG
                     RYQTPRLRPGVYVVIAWAPGFASQHVIVPLPVGGAAEANFALQPLEPMHPGVIEGRVI
                     DRLTGDPVAGAWVFYHPAVDDKAIAPHDAPDAAHPRVRTNARGEFWISPLRPGRYHLA
                     VRADGFEPAHKNVNVEAGEVTAVRFLLEPKRPPHPGAIVGQVVDGETGDPVPGARVFF
                     GRPLDDDDTVEWWRKHDAATDHLNFVETDHQGFYRIKPLRPGAWHLLVRAAGYQPAAR
                     KVVVRPGQTVEANFKLRPNDPRDPGSIVGRVTDGTSQTP"
BASE COUNT         1389 a         3597 c         2607 g         1400 t
ORIGIN      
        1 cgcccgccat gatctggccg agatcagccg ccagcgcgcc gagctccacg accggctcga
       61 ggagacccgc ctggcgctgg aggacgcccg gcgcgaggcc gccgcccagg gcgagggccg
      121 cgcgcaggcc gtcgccgccg tccgccgcga actggaggac cacctcgccg aactcggtca
      181 actgaccagc aaacaggaag tccagctcgc ccgcctgcgc aaggaactcg gagacctgcg
      241 caaggaatcc aacgccctcc tgctggaaaa acgtcacggc gaggacgtca tctccaagct
      301 gatccactcc ctcacctact gccagaacaa ggcctgggaa cagtcgacct gggtcgatga
      361 gcaactgcgc agccgcggtt acgtgctcgg cctctacttc gaggcggtcc gccgcaacaa
      421 ggcccgggcc ctcctgaccg ccgtgcgctg ggtcgcccgc cgcctcctcg gccgtggatt
      481 ctcatatgcc cgggccatgg gcgtcgagta ccggcttcat acgatcaagg gggagttgca
      541 ggcgctgtcg ttctacgccc gcgacgccga acatcaggtc tccgacctcg ccatcgcctc
      601 gcccaccagc atggccggcg gcctgctccc ctcggcccag cggcccgagc ccgacctgat
      661 cgtctactcg atcaccaact gggattttcg catgcagcgg ccgcagcata tcgtctcgcg
      721 cctcgtccag gccggcagcc gcgccttcgt catgcgcgat accacgatcg gcctggagga
      781 ggaattcccc agcgaccaga cgatcgacaa tcacatgctc gtccgccccc tggggcgcga
      841 tctgtgggag gtccagccct gcacctacca ccccctgaac atctacaagg acaatctggc
      901 cgatccgcgc gacatggcgc tcctcaagcg ctcgctgggc ctgctgcgcg agcgcttcag
      961 catcggcgat cacgtcgccc tcgtgcagtt gcccttctgg gggccgctcg ccttcgccct
     1021 caccggcgcg cgcgtcatct atgactgcat ggataatcac agcgacttct ccaccaacga
     1081 ccccgccatg ctccagcagg aggagcacct gctcacgtcg gccgatgccg tcgtcgtcag
     1141 ctcggcttat ctggacgagc tgacgggcag caagccgcgc gtgaaatacg tcgtccgcaa
     1201 cgcctgcgaa ttcgattatt tcagccgcga gcccgattcc ctcgatcccc tcgtccgcga
     1261 ggcggagcag cccgtcatcg gttactacgg cgccatctcc gactggttcg acgtcgacct
     1321 cgtcgaatcc gtcgcccgcc gccggcccga ctggcgcttc atcctcatcg gctacacctt
     1381 cggcgccgac atcacccgcc tccaggccct gcccaacgtc agcttcaccg gcgagatccc
     1441 ctacagccga ctgacttcct tcctctatgg cttcgatgtc tgcctgatcc cgttccgcct
     1501 caccgaactg atccgggcca ccaacccggt caagatctac gaatactccg ccgccggcaa
     1561 accgaccgtc gccaccccga tccccgaggt cgtcgccctg ggcgacctgg tccgcaccgc
     1621 cgccgacgcg cgcggcttcg agcaggcgat cgccgacgcc ctcgccgaaa ccgacaaacc
     1681 ggcccgcgcc ctccgtctgc gcgagttcgc ccagcacaac acctgggatg cacgcgtcga
     1741 ggatttcaaa cgcatcatcc gccgtcacgc gttccccaag gtcagcatcg tcatcctctc
     1801 ctataacaat ctgcgctata cgctcgattg cctcgaaagc atcctcacgc agtcctccta
     1861 tccgaacttc gaagtcatcc tgatcgacaa cgcctcgccc gaactcgagg tgcgcgaggc
     1921 cctgggcaag ctcgaggacc cgcgcctgcg catcgtcctg aacgaggaga acaagggcta
     1981 cgccggcggc aacaaccagg ggatggcgat ggcccgcggc gatatcatca tcctgctcaa
     2041 caacgacacg atctgccccg ccgactggat cgaacggctc gtcgaaccgc tgcgcgacaa
     2101 ccccagactc ggcatggtcg gaccaatgac caattcggcc ggcaatgagc agatgctcga
     2161 tgtcttctgc gccggcgcct ccagccagca tacctggctg cgcgacttct accgcgtccg
     2221 ccgcgggcgc cgccatctca ccgaccgcct cggcttcttc tgcgtcgccc tccgccgcga
     2281 actctacgaa cagatcggcg acctcgacga gggctttgaa ttgggctact tcgaggacga
     2341 cgactactgc ctgcgcgccg tcgcggccgg ctggcagctg gccgtcatcg aggacgcctt
     2401 cgtctaccac cacggctcgg tcagctttaa ttcgattgac accgagcgca ggcgcgtcct
     2461 gcaggagcgc aatcgccggc gcttcgaaaa gaagcatcag cgtcgctggg cgctgccgat
     2521 ctgcgagatc ctctactacg gcgccgaggg acccgcccat gaacccgatc gccccaccgc
     2581 cctgatcggc ccctgcaccg attggagcgg cccgctcggc cgtccgcagc agatcgcggc
     2641 cgcgctggtc cacgccggct tccgtgtctt ctacgtcacc aacaatctgc gccacgaccg
     2701 cttcctcggc atccgcatgc tctgtccgtt cctctacatc gtcgaaaacc gcgatcaatg
     2761 gctcaccgac atccagcccc agccgctcga cctcttcatt tcgttcggcc cccgcggcga
     2821 ctcgatcacc acgaaggtcc gcgcgcgcag ctacctgatg gacttcgacg cctacccctt
     2881 ccgcgagtcg tggaaatgga acccgtccga cccggccgct ctggccgtcg ccgccaaggc
     2941 cgattggatc accgtctccc aggacttcct gacgggcgag ctgctcggcg tcccccatca
     3001 gcgcgtccat ctgctgccca atggcgtcgt cgccgcgcac tggtcgacca acaagccgtt
     3061 gacgccgccg gaggaaatcc gcgagatcct gcgcaagcag ggcggcccgc tcatcgccat
     3121 cctcggcgtg ctcaacgacg gctcggtcaa cctgaactgg ctccgcgagg tcgccgacgc
     3181 ccgccccgag tggagcttcc tcttcatcct ggccgacgaa tcgaccggcg acgacgacga
     3241 gcatgatttc ttccgcctgc gcaacgtcca cctgctcggc tggaagcccg tcgccacgct
     3301 ccccgattac ctccatttcg ccgatgtcgg cctgctgcct cccgccacgc tcgccgacaa
     3361 tcgcatctat ccgcgcgcgc ttctgctccg gctcatcggc gccgggctgc cggtggtctc
     3421 gggctatccg gtccacgagc cgcagttcac cgggatcgtc acgcacgccc cgcggcccga
     3481 ggatgccgtc ctcgccatcc gccaggccat gggcaaggcc cacctcgaac cgttcagccg
     3541 gcgctgcgcc gagctcgtcg ccgccgagga ttggagcgtg ctgatccggt cgccgctcgc
     3601 cgccatcgag cccgtcctca aggaacggcg gtcccgaccg gcgtgaccga gccttttccc
     3661 gcgaccggcc ccggttccgg ctcgcaacgc ctcctcggag cgaacccatg gcgggctggc
     3721 atctgctgac ccgattgcag gccaactggc ggcgttacct gcgccggcgc gatcacggcc
     3781 gccccgccag cctcgccgcc ctgcgcgcct gggccgtccc cgatcgggtt tcgatcgtcc
     3841 tgcccacgta caacggccgc cggcatctcg acgaagccat tgagtccatc cgcgcgcaga
     3901 cctggcccca ctgggaactg atcctcgtcg acgacggctc gaccgacggc accggcgacc
     3961 tggccgacca gtgggcgcgc gccgaccccc gcatccgcgt catccatcag gccaaccagc
     4021 gcctgcccgg tgcgctcaac accggctttc gcgccgcccg cggcgagttc cacacctgga
     4081 ccagcgacga caaccggctc cgccccggct tccttgccgc ccttgtcgcc gttctccgcg
     4141 agcgccccgc cgccgatgcc gtctacaccg actacgacct gatcgacgag gcgggacggc
     4201 ccctgctcga ctcccctgtc tgccgcgcct tgcagtcgcc ccccggcgcc gcccgcctcc
     4261 gcctcccgcg ccagcccttc gatctcaatt tcgtcaacgg caactacctc ggcggcgcct
     4321 tcctctaccg cgcccacgcg acgcgcctgc tctggggccg cgaatgggac ggcccctaca
     4381 gccccaaccg cttcctctgc gaggattacg acttcttcct gcggctcaac gcatggctcc
     4441 gcctcgaaca ttccggcctg gaacagagcc tttacgagta ccgcctccat tccgactcgc
     4501 tgaccgccac cgcgcgccag gggcgcgtcc gcgagctgag cgaagaactc ctgcgcttcg
     4561 acgacttccg ccgcgacatg acgctctggc ccatcctgtg gcacatcgag tcggtcgacg
     4621 atcccacgcg cctcggctcg cgcctgcgcc tgcgcgccct cgatgtcggc gatcacgtga
     4681 ccgccgagcg cgaagccccc ccctggctgg ccgccgtctg ggtccgcgtt gacaactccg
     4741 aaacccagcc cggctgcgat gtccccgccg gctgggcgcg cgtcctcgtc tgccgccggc
     4801 ccccccgcgg cgacctctcg gattgggacg tgatcctgac cgaggcgaaa ccgggcgacg
     4861 atccgccgcc cggcgccccg atcctgcgct atcgcgacga ggaagcgctc tggcacgcgc
     4921 tgcgcgtggc cgcctggcaa cgtcaggccg tcgccgccga ggcccgcgcc ttcgatgaaa
     4981 ccccgccccg ccatcgcctc tcggtcgtcg tcggcaccaa ccggccttcg ctccgcctcc
     5041 gcccgctgat cgattcgctc gccgaccaga ccgcccccaa gtccatctgg gaactgctgc
     5101 tcgtcgataa cgcgcgcgga cgccacccgc gcgaattgac gtccggtctg ccgcgcctgg
     5161 ccgagctccc gcacgtccgc agcctgtcca atcccgtccc gggcctgtcg atcacgcgca
     5221 atgccggcct cgccgaggcg cgcggcgcaa tcgtcctgtt cctcgatgac gacgtgacgc
     5281 tcgcgccgac ggcgctggcc gcgctcctcg ccgcctgggc ggccgttccc gacgccggcg
     5341 tcatgggcgg cgcgatcgag ctggcgcccc tcgaccggcc gccccgctgg ctcggcccgg
     5401 ccgtcctgaa atactggggc ggcctcgccg cgggccttcc cgcccgccac ccggccgcca
     5461 cctggcgcga gctccccttc ggcggcttct ggagcgcccg cgccgcgctg ctgcgccgca
     5521 tcggcggctt ccgcgggcgt tatggccgcg ccggcctcgc gaccgacgcc ggcgaggaga
     5581 tcgtggccgc cctccagctc cgcgccgacg gccgcgcaag ctggcttgag ccggccgcgc
     5641 gcgcgctcca tcacgtctcc cgcgatcgca tcaccctgcc cgccttcttc cgcatccgga
     5701 agaccaacgc cgacacattc ttcctcctca gttccgaggg gcgcatcgac gacgatcccg
     5761 gtctgagcgc cagcctccgc agcggcctgc gccagatcct gcgcgccctg gcccccgtgc
     5821 cccgcccgct cgacgtccgc ctcgatcacc tcggcaacgc cgtcgaacac ctctggctcg
     5881 cccaccgttt attctattac cggctcggtc gtctccggcc gggctcgccc cccgccgccc
     5941 cccgccaatg aaataaagcc tccgcgccgc gtgtctctcg tcctcagagg ccttggcagg
     6001 ctcgatccac ggcgccgcgg cgccgcgctc ccggcgccgg gcacaatccg cccggggcgg
     6061 gcgcggctgc cttgcgccat caatccccga ccccttccga cccatcggct gaacgcagtt
     6121 aggagagatg tcagatgcgc tgcaacgctt tgcgccatgc catccgccgc ggcttgaccc
     6181 gtggttggat cacctcgatg ctgctcgcgc tcgcgctcgg cttcacgccg ggcccggacc
     6241 tttccgccca cgacgacgac cgggccgaga tccgcggcca tgtcaccgac gccgtcaccg
     6301 gcaaaccgat cgagggggcg aacgtcttcg ccgttcgcga taagagcaat tccagtctcc
     6361 agcgcaagac cgatggcaag ggaaaatacg aattcaagca tctggaaccc ggcccctggg
     6421 tgatcgaagt cggcaagccc ggctatgccc cgcagcgcgc cgaagtggtc ctgcaacccg
     6481 acaagcgact cgaactcgat ttcgctctcc agccgaacat ggacgacgtc ggcgaaatcg
     6541 ccggcgcggt gatcgacaac gaaactggcg aacccgtccc cgacgccttc gttttcgtcc
     6601 gcgatcccct taaggacggc ccccacgacg tcatcgggga ttccaccgat gaacatggcc
     6661 gctactcgat ccccggcctg ccggcgggca ggtacgaggt ccacgccgtc catcccgatt
     6721 acgagcgcgc caaaaaaacg gtcgaggtcc acgccggcct gttgtcggtc ctcaacttcg
     6781 atctgacccc ccggcaggag cccggtaccg gcagcctcct tgtccgtgtc gccgacgccg
     6841 acaccggcct gcccgtcgcc gaggccatcg tcacgatcga aggcattttc acctttgccc
     6901 ccctgctgac cgacgaaaac ggcgagctgg cctttgattc gctcgcagcg ggcgagcggc
     6961 gcgtccaagt cgacaagcag ggctacgagc atcagcagca gatcgcccgg atcgaagccg
     7021 gccggcagac cctcctcgag ttcgaactcg tcccgctctc cggttcgcgg ctgggcgcca
     7081 tcgccggccg cgtcatcaac ggcgcgaacg aagcccccat cgaacacgcc cgcgtctact
     7141 atcgtctgat cgagcacgac gacgacgacg atgacaagtc gcacgatgcg tcgcccgatg
     7201 gccccttcgt cgagaccggt cccgacggcg tctacgaaat ccccgacctg cgccccgggc
     7261 tctatggcct gcaggtcgtc gccgaaggct tctattccca gtggaacaag gccgaggtca
     7321 agccgggcga gacgacgcgc gtccccttcg ccctgcgcct catcgtcccg gccgaagccg
     7381 gcttcctggc cggccaggtg accgacgcgg cgaccagcga gcccgtcgtc ggcgcgcgcg
     7441 tgttcttcgc gcgccaggat gaagtcgaca agaccatgat gctcgacgcg accgacgaat
     7501 cgcgtccgca cgtcgtcacc gatgaaaacg gcaactacga aaccccgccg ctgcggccgg
     7561 gccagtacct cctcgtcgtc cgcgcccgcg gctacgagcc cgcgcgccgc atggccgagg
     7621 tccgctccgg cgaaaccacc gaggtcaatt tcgcccttgc ccccttcgtg ccggtcgaac
     7681 ccggctcgat caccggcctc gtcctcgatg gcctcaccag ccagccgatc gccggggcgc
     7741 tgatcttctt cgcgccggtc gtccccgatg cgcccgacgg ctcggtggcg ccccatgacg
     7801 atctggccga ccacctcccc tcggtccgca ccgacaacac cggccgctat cagatcgacg
     7861 acctgcgtcc gggacgctat cgcctcatcg cacgggcccg cggttatctg ccggcgacgg
     7921 cgcgcgccga ggtccaatcc ggccagagca cccgcgtcaa cttcgcgctg cgtcccgatc
     7981 ccaatcccga cgccggcgtg attcaggggc gcgtgatcga ccgcgaatcc aatctgcccc
     8041 tcgccggcgc cttcgtcttc ttcgcgcgcc tgccgatcaa caccgtcgaa ccccccgccg
     8101 cgctcaacga gttcggcgcg atgatgtcga ttgaaccccc cgccggcggc gacgactacg
     8161 tccgcaccaa cggccgcgga cgctaccaga ccccgcgcct gcgccccggc gtctatgttg
     8221 tcatcgcgtg ggcgcccggc ttcgcctcgc agcatgtcat cgtgccgctg cccgtgggcg
     8281 gcgccgccga ggccaatttc gccctccaac cgctcgaacc gatgcacccc ggcgtgattg
     8341 aaggccgcgt catcgaccgg ctcaccggcg accccgtggc cggcgcctgg gtcttctatc
     8401 atccggcggt cgacgacaag gcgatcgcgc cccacgacgc ccccgacgcc gcccatcccc
     8461 gcgtccgcac caacgcccgc ggcgaattct ggatctctcc gctccgcccc ggacgctacc
     8521 acctggcggt ccgcgccgac ggcttcgagc cggcgcacaa gaacgtgaac gtcgaggcgg
     8581 gcgaggtcac cgcggtccgt ttcctgcttg aaccgaagcg gccgccgcac cccggcgcga
     8641 tcgtcggcca ggtcgtcgat ggcgaaacgg gcgacccggt ccccggcgcg cgcgtcttct
     8701 ttggccgtcc gctcgacgac gacgacaccg ttgaatggtg gcgcaagcac gacgcggcga
     8761 ccgaccacct caacttcgtc gaaaccgacc atcagggctt ctaccgcatc aagccgctcc
     8821 ggcccggcgc ctggcatctg ctcgtgcgcg ccgcgggcta tcagccggcc gcccgcaagg
     8881 tcgtcgtgcg cccgggccag acggtcgagg cgaatttcaa gctccgcccg aacgatccgc
     8941 gtgatcccgg ttcgatcgtc ggacgcgtga ccgatggaac cagccagacg ccg
//
LOCUS       DAOPCO010000160         8965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7319_length_8965_cov_4.625028, whole genome shotgun sequence.
ACCESSION   DAOPCO010000160 DAOPCO010000000
VERSION     DAOPCO010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8965
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7319_length_8965_cov_4.625028"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLS90_11235"
     CDS             <1..279
                     /locus_tag="PLS90_11235"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPK03019.1"
                     /translation="GENMLPEGVTSVAGGNDPINYGGQVIANPLLDSDGIHLTEGSPA
                     INAASDIGVTDDFDGGARPYDGGFDFGADEFGSEPPPPPNSVRHWNPY"
     gene            534..1184
                     /locus_tag="PLS90_11240"
     CDS             534..1184
                     /locus_tag="PLS90_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03020.1"
                     /translation="MFEPGRHLQGAFQDARMQEQIMRRAFTLIELLIVVAIIAILAAI
                     AVPNFLEAQVRAKVSRAKSDMRSIVTALETYRVDNTGYPSFEGLTNLEDNARYLNSPL
                     WILTTPIAYMTTLPAEAPFGHVKAPNYPNQYFRGYYYIGGDYWTFWAPSLISTYHQYP
                     EPYINASYVLSAIGPTQQLTIPGIRWPYDPTNGTVSRGDILYVGGSGAGAGFHSFN"
     gene            complement(1449..2306)
                     /locus_tag="PLS90_11245"
     CDS             complement(1449..2306)
                     /locus_tag="PLS90_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK03021.1"
                     /translation="MKKGLSYWSFPGGLAGNFPIVEAARLAREAGYQGLELALSADGE
                     LTPQTSDAEVERLRDAVIGEGVEIAGLASALTWETLLTAEEPRERQAAVELVRRGLQV
                     AAMLGVDAFLVIPGAVDVFFRPDLAGISYQAAFDRLRGALDELIPVAEELRVALALEN
                     VWNRFLLSPLELRDLVDSCQSPYVGVYLDVGNVMLTGYPEDWIRILGQRIHRVHFKDF
                     NRAVGTAEGFVDLLAGDVNWPAVMASLREIGYDSYCTAELIPCYRYAPEMRWWNTSRS
                     MDAIFALGQ"
     gene            2644..2784
                     /locus_tag="PLS90_11250"
     CDS             2644..2784
                     /locus_tag="PLS90_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03022.1"
                     /translation="MRPRRWGEGSRSAPGGGGGYDPLADAERWEPAAGERALLILRRR
                     AP"
     gene            complement(2815..2979)
                     /locus_tag="PLS90_11255"
     CDS             complement(2815..2979)
                     /locus_tag="PLS90_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03023.1"
                     /translation="MHLIRRFIQWWRSLLHQAGGGSEFLCDTCRYDHGSACTRPERPN
                     ARRCPDYKRK"
     gene            complement(3007..3468)
                     /gene="gspG"
                     /locus_tag="PLS90_11260"
     CDS             complement(3007..3468)
                     /gene="gspG"
                     /locus_tag="PLS90_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPK03024.1"
                     /translation="MNHRLDRRSIRRAHPRGFTLVEIILVVVIVLTLAAVVTPRLVGK
                     SKQAKINTTKIQMNSLKTSLQNFEINAGRFPTTAEGLEALVRKPSGLSDSEWPEKYVD
                     KVPKDAWGNPFEYTCPSEHDLDYDLVSAGPDGQFGSDDDIANYDEDGGDSL"
     gene            4044..4550
                     /locus_tag="PLS90_11265"
     CDS             4044..4550
                     /locus_tag="PLS90_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03025.1"
                     /translation="MFWILVLLLFIGILFFLMEILLPYGLSFLAGVIVIGFGAWLAFR
                     HFDPGLGMLYLLAAGLISGGAAWWLFSSGERLLALPPPSPDTAPPPQADTDRRPPIGA
                     LVDVVQPLRPTGTILWNGRRHAARSLIQEAESPIGARVRVTDYDSTFVVVEPLRDDPA
                     RPEPPPHA"
     gene            complement(4584..5756)
                     /locus_tag="PLS90_11270"
     CDS             complement(4584..5756)
                     /locus_tag="PLS90_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK03026.1"
                     /translation="MRITQNALFNQLRHRMESNSARLMRAQEIVATQKKLNRLSDSPV
                     DGARVVDVKQSLGRIDQYLRNLDRLDAVVQVQDATLGEIGDLLVRAKELLLQESNEVT
                     STKETREAARLEIVALLGQLTQIANTRFNGESIFAGSRTGGPAFVGATVATAPNGVSG
                     GAAVTAAKVYDDAALIPGEYRLVFTAPDQFELREAVSGDLILAGQPYVSGQPIRFHGV
                     ELVVTNGSGAPAAGDRFDVTISPPGVYQGDSQVARVDIQADTRADKNIPGDRLFQGAG
                     VANGVDLFDVMNRLNAALRTNDRAAMDGLLNDLDAARVQVSNERTRVGAQTNLLEGVR
                     QRHLDVQLSLEAVRGNLEDADLAEAITQLTQQQTIYEATLGAAAGIVQPSLLDFLR"
     gene            complement(5776..7413)
                     /gene="flgK"
                     /locus_tag="PLS90_11275"
     CDS             complement(5776..7413)
                     /gene="flgK"
                     /locus_tag="PLS90_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPK03027.1"
                     /translation="MSLFGVLNTGRTGIFVSQHGLATTGNNIANVNTPGYSRQRLMQA
                     ARTTGGVQSLGVERLGDVFFAGRLRESNTRMGGLQTLSLNLQQLETALGDTDGSGFSK
                     ATEQFFAALQDLSVTPGGPAEREAVRSRGSQLVASLRGLGGQIREQRQQLDAQLRQQV
                     EQINTLARNLADVNRRLDDAGPGADEARNALLDQRDQIVNQLSNFLPVRTLEDAGGKL
                     TVFVGDEVLVEGLATRALQLTPNPANDGLHDLAVIDAAGNVRAMRKAPTEGSLGALLR
                     ARDEFAGKALADVERLAGRLIQSFNAIHRQGVGLDGGGGRDFFAGLDVTAAPDGRNRG
                     GVGVAATAISDADLLTFDDYEIRFTSADAYEVVNTRTNAIVTSGPYVAGGTIQFDGMS
                     VELNGPAGPQAGDVIHVDSYRGTSRRMALAPEIAASLEAIAAGGTDAAGDNTQALLLA
                     ALRDQLTMGSPPTQTFDGYFGAIRLQVALATESSLGGVEDEAVTQQQLVTLQQSVAGV
                     SLDEEATNIIQYQRAFEASSRVIRIADELIETVLQIV"
     gene            complement(7447..7971)
                     /locus_tag="PLS90_11280"
     CDS             complement(7447..7971)
                     /locus_tag="PLS90_11280"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPK03028.1"
                     /translation="MSEQPYESLLTLLGEERAIYEEMADLLDQEREAMLAMAVDRLAE
                     IVARKETLALRIKALDESRRVLARRLGAALGLPPHELTISELYAAAPAEIAGRLRAVG
                     AALREVVVRCREINQFNERAARRGLELVGGAIQTLLEQADPTGKVYEAPRPRARGYGG
                     PVAPGGGGIFSRQA"
     gene            complement(8049..8363)
                     /gene="flgM"
                     /locus_tag="PLS90_11285"
     CDS             complement(8049..8363)
                     /gene="flgM"
                     /locus_tag="PLS90_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPK03029.1"
                     /translation="MKIQGRKSNPVSPLGPFAGPPQVDEASPTASPAAGDSVELSSTR
                     ELQRLNQMAQAVPTVRLEKVEGLRDAIEEGSYYVESEKLARRVVDEALTQAILGKAPS
                     AC"
     gene            complement(8461..>8965)
                     /locus_tag="PLS90_11290"
     CDS             complement(8461..>8965)
                     /locus_tag="PLS90_11290"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HPK03030.1"
                     /translation="IWFSRASDCGSFIDPGRALDLNDPQSLARLNQIEDPRQRAEAVA
                     TQLETVFLTMMVKAMRQTVPEGGLLGKGLGGKHYVEMLDQEFARAGAAGGLDPRLHQA
                     LVRQMMQTPEAIGTATVSSPAAPEPAGGATDLPTKGDAPTAPEASGADYETRKGTVRR
                     VARENML"
BASE COUNT         1456 a         3087 c         2744 g         1678 t
ORIGIN      
        1 ggcgagaata tgctgcctga aggcgtgacc agcgtagctg gaggtaacga ccccatcaat
       61 tatggcgggc aggtgatcgc caatccgctg ctcgacagtg atgggatcca tctcactgaa
      121 ggttctccag ccatcaacgc ggcatccgat ataggcgtga cggatgattt cgatggcggt
      181 gcaaggccat acgacggcgg tttcgatttt ggcgccgacg aattcggctc ggaacccccg
      241 ccgccgccca actcggtgcg ccactggaac ccttactgag atatcttaac ccggcacaag
      301 cccatgatcc gaaatcatcg gatcatgggc tttaaaattt ggagaggaag gataaatgcc
      361 cttcgaccaa cgcgctgagc caatgactgg tggatctggc cacaagcggg atcgtgcaag
      421 ctgccgggcg agggcagtgg ccgtcgacgg cggaagtgat ttgagcggcg taaatggctc
      481 atcggtatct tttctgatgc ctgtaaattc tcacgaaatg aaatattcac ttcgtgttcg
      541 aaccaggtcg ccatttgcaa ggcgcctttc aagatgccag gatgcaggaa caaatcatgc
      601 ggcgcgcttt cacattaatc gagttgttga ttgtggtggc gattatcgcc atcctggcgg
      661 ccattgccgt acccaacttc ctcgaggcac aggtgcgcgc caaggtcagc cgggccaagt
      721 ctgacatgcg ttcaatcgtc acggccctgg agacttaccg tgtcgacaac accggctatc
      781 caagcttcga agggcttacc aatctggagg acaacgcccg ctatcttaat tctcccctct
      841 ggattctgac gacccccatc gcttacatga caaccttgcc cgcggaagcc cccttcggtc
      901 atgtgaaggc gccaaactac cctaatcagt acttccgcgg ctattactat atcggcggag
      961 attattggac tttctgggcg ccgagtctta tctccaccta tcaccagtat cccgaacctt
     1021 atatcaacgc atcctatgtc ttgtcggcga tcgggccgac ccagcaactg accatccccg
     1081 gcattcgctg gccgtacgac ccaaccaacg gcacggtcag ccgcggcgac atcctttacg
     1141 tgggcggatc cggcgcggga gcgggcttcc attcgttcaa ctgaatcttt ccgaatactg
     1201 gggaaaactg gtgacaggaa aaagacttgg cttttatcta taaattgagg cttgagagga
     1261 tcgcccagat cccgattgac ccgatacctg caaatcctgc tgatggaggc ctacggtacg
     1321 aacgtagggc atcgaaccat caattcaatt ccttgtcggt aggccgggca tcggcggccg
     1381 cggacagtcc ttcgtcggcg gaagccaggg actgatcgcc gatccatgcc cggcgctctc
     1441 gggttcagct attgtccgag tgcgaagatg gcatccatcg atcgcgatgt gttccaccat
     1501 cgcatctcgg gggcatagcg atagcagggg atcagttcgg cggtgcaata gctgtcatag
     1561 ccgatctcgc gcagggacgc catcaccgcg ggccagttca catcgcccgc cagcagatcc
     1621 acgaaacctt ccgcggtgcc cacagccctg ttgaagtcct tgaaatgcac gcgatggatc
     1681 cgttggccca gaatgcgaat ccaatcctca gggtagcccg tcagcatcac attcccgaca
     1741 tcaaggtaca cgccgacata cggactttgg caggaatcca ccagatcgcg caattcaaga
     1801 gggctgagca gaaagcgatt ccagacattc tcaagagcaa gcgcgactcg gagctcctcg
     1861 gcaacgggaa tcaactcatc cagcgcgccg cgcaggcgat cgaatgcggc ctggtacgag
     1921 atcccggcga gatcgggacg gaaaaataca tcgacggcgc cggggatgac caggaatgca
     1981 tcgacaccca gcatggcggc cacttggaga ccacgtcgca ccagctccac cgcggcttgg
     2041 cgctcccgtg gctcctcggc ggtcaacaac gtttcccatg tgagggccga ggccaggccg
     2101 gcgatctcaa ccccttcgcc gatcactgcg tcccgtagac gctcgacctc cgcgtctgag
     2161 gtttggggtg tgagttcgcc atccgcgctg agcgccaatt cgagcccctg atagcctgcc
     2221 tcgcgcgcca ggcgcgccgc ctcgacaatt ggaaaattgc ccgcaagccc gccgggaaac
     2281 gaccagtaac tcaagccttt tttcacctta agccccccct tcttgcccgt gttttatcga
     2341 gcggtttcat ctgtcgatgg tccaattgac gggcttgctc cgtgcgtcgg ttctcgctcc
     2401 tccagcgctc atccactgga tcggttgcaa gtcggtagcg ccagttgcta ctcaatcgga
     2461 agcggtggtg ctgttctcaa gcgatgtgcg cagcacgggg gcattaaata cgaggaaatc
     2521 tcccatctca ttggccgcca caacaaggct tgaggttaac cgatgttccg gggtaactca
     2581 cgttcttttg agacgttctt gagaagttgc accagggatt gttatcggcg gttttccgtc
     2641 gaaatgcggc cgcgccgatg gggcgagggc agccgatccg cgccgggtgg cggcggcggc
     2701 tacgatccgc tcgccgacgc cgaacggtgg gaaccggcgg ccggcgagcg cgcgttgctg
     2761 atcctgcgcc gccgggcgcc gtagtgatgg cgacgtccgc gaccggctgc agcgttactt
     2821 ccgcttgtaa tccgggcagc ggcgggcgtt ggggcgctcc ggccgcgtgc aggcgctgcc
     2881 gtgatcgtac cggcaggtat cgcacaggaa ttcgctcccg ccgcccgcct ggtgcagcag
     2941 actgcgccac cattgaataa aacgtcggat cagatgcatg ctgcgcggag ggcgacgcgc
     3001 gcgccgttac aggctgtcgc cgccatcctc gtcgtagttg gcaatgtcgt catcggagcc
     3061 gaactggccg tcggggccgg ccgacaccag gtcgtaatcc aggtcgtgtt ccgatgggca
     3121 ggtgtattca aatggattgc cccaggcgtc cttgggcact ttgtcgacgt atttttcggg
     3181 ccactcgctg tcgctcaggc cgctcggctt gcgcaccagc gcctccagac cctcggcggt
     3241 ggtggggaac cgcccggcat tgatctcaaa gttctgcagc gaggtcttca gcgagttcat
     3301 ctggatcttg gtcgtgttga tcttcgcctg cttggatttg ccgaccagcc gcggcgtgac
     3361 gacggccgcc agcgtcagca cgatcacaac caccagaatg atttccacca gcgtgaagcc
     3421 gcgtgggtgg gcgcggcgga tggagcgacg gtccaatcgg tgattcatgc gggaatcccc
     3481 ctcgcattgt cggcggccgg acacatccgg ctgccatcct catcttgcca cagcccgacg
     3541 ggtggcatca agacttggtc ctccgcaaca gggcaagatg aatgccagcg gcgagccgcg
     3601 tccgcggcgg cggcggttgc ggggctttcg gccattccgc tgatattcgc cacgcgctgg
     3661 cgcgccgcgg ccggcgagac gcattgtgaa ttcgcgactc ccgctccgcg attacgggaa
     3721 cgacgctctc gggagccagg gacgagcgac gagaaccttc ttggtaaggc ctccgggggc
     3781 gatcacgagt tgcccatgaa cccgcgactc accatgaggg ataaatccgg caactgggtc
     3841 agcgccatgc gcagcgccgc caggcagccg agcgcctcgc gcgtctcgcc cacacctctc
     3901 tcggttttcg tcctcggctc tcgaatccgt tttcgctggc gttttcaccc gctcacgcta
     3961 aataaaggtt ccggggcgcg tagcgcccgc ccggtcccgt ccccgttctt cgcgggcatc
     4021 ccgactcccc cttcgagcgc cgcatgttct ggattctcgt cctgctcctg ttcatcggca
     4081 tcctcttctt cctgatggag atcctgctcc cctatggcct gagcttcctg gccggcgtga
     4141 tcgtcatcgg cttcggcgcc tggctcgcct tccggcactt cgatcccggg ctggggatgc
     4201 tctatctgct ggcggcggga ttgatctcgg gcggcgcggc ctggtggctc ttcagttcgg
     4261 gtgagcggct gctggccctt cccccgccgt cgcccgacac cgcgcctccc ccgcaggccg
     4321 acaccgaccg ccggccgccg atcggcgccc tcgtcgacgt cgtccagccg ctgcggccga
     4381 ccggcacgat cctgtggaac ggtcggcgcc atgccgcccg ctccctcatt caggaagccg
     4441 aatcgcccat cggcgcgcgc gtccgcgtga cggattatga ctcaacgttt gtggtggtgg
     4501 aaccgctgcg cgatgatccg gcgcggcccg agccgccgcc gcatgcctga cttgattatc
     4561 ccgccgcccg ccgcccggcc gtgctatctc aggaaatcga gcaggctggg ctgcacgatg
     4621 cccgccgccg cgcccagcgt cgcctcgtag atcgtctgct gctgcgtcag ctgcgtgatc
     4681 gcttcggcca ggtccgcgtc ttccagattg ccgcgcaccg cctccaggct gagctgcacg
     4741 tccagatgac gttggcggac cccctcgagc aggttcgtct gggcgccgac gcgcgtgcgc
     4801 tcattggaga cctgcacgcg cgcggcgtcc aggtcgttca gcaggccgtc catcgccgcc
     4861 cggtcgttcg ttcgcagcgc cgcgttcagc cggttcatga cgtcgaacaa atcgacgccg
     4921 ttcgccacgc ccgcgccctg gaacagccgg tcgccgggga tattcttgtc ggcccgcgta
     4981 tcggcctgga tgtcgacgcg cgccacctgg ctgtcgccct ggtagacgcc ggggggagaa
     5041 atggtcacat cgaaccggtc gccggccgcc ggggcgccgg aaccattggt cacgaccagt
     5101 tccacgccgt ggaagcggat cggctgaccc gaaacatagg gctgcccggc aaggatcaga
     5161 tcgccgctga ccgcctcgcg aagctcaaac tgatcggggg cggtgaagac cagccggtac
     5221 tccccgggga tcagcgccgc gtcgtcgtaa accttggcgg ccgtgacggc ggcgccgccg
     5281 ctcacgccgt tgggcgcggt ggcgaccgtc gcgccgacga acgccgggcc ccctgtccgc
     5341 gaccccgcga aaatcgattc cccgttgaac cgggtattgg cgatctgcgt cagttgcccg
     5401 agcagcgcca caatctccag ccgcgcggct tcgcgcgttt ccttcgtcga cgtgacctcg
     5461 ttggattcct gcaggagcag ttccttggcg cgcacgagca gatcgccgat ctcgcccagc
     5521 gtcgcgtcct gcacctgtac gaccgcatcg agccggtcca ggttgcgcag atactgatcg
     5581 atgcgcccca ggctctgctt gacgtcgacc acgcgggcgc cgtccaccgg gctgtcgctc
     5641 agccggttga gcttcttctg cgtcgcgacg atctcctgcg cccgcatcag ccgcgcggag
     5701 ttgctctcca tccggtggcg gagctggttg aacagggcgt tttgtgtgat gcgcatcggt
     5761 cttctccccg ccggcctaca cgatctgcag tacggtctcg atcagctcat cggcaatccg
     5821 gatcacccgg cttgaggctt cgaacgcccg ctgatactgg atgatgttgg tcgcctcttc
     5881 atcgagcgag acccccgcca ccgactgctg cagcgtcaca agctgctgct gcgtgacggc
     5941 ttcgtcctcg actcccccca gcgagctttc ggtggcgagc gccacctgca gccggatcgc
     6001 gccgaaatag ccgtcgaacg tctgcgtggg gggcgagccc atcgtgagct gatcgcgcag
     6061 cgccgccagc agcagcgcct gcgtgttgtc ccccgccgca tcggtcccgc ccgcggcgat
     6121 cgcctccaga ctggccgcaa tctcgggggc cagcgccatc cgcctgctcg tcccgcggta
     6181 gctgtcgacg tggatcacgt cgccggcttg cgggcccgcc ggcccgttca gctccaccga
     6241 catcccgtca aactggatcg tgccgccggc cacgtacggg cccgacgtca cgatggcgtt
     6301 cgtccgcgtg ttgaccacct cgtaggcatc ggccgaggtg aagcggatct cgtaatcatc
     6361 gaacgtgagc aggtccgcgt cgctgatcgc ggtcgcggcg acgcccacgc cgccgcggtt
     6421 ccgcccgtcg ggagcggccg tcacgtcgag cccggcgaag aagtcgcgcc ccccgccgcc
     6481 gtccaggccg accccctgcc gatggatcgc gttgaaactc tgaatcaggc gcccggcgag
     6541 gcgttccacg tcggccagcg ccttgccggc gaattcatcg cgcgcccgca gcagcgcccc
     6601 caggctcccc tcggtcggcg ccttgcgcat cgcccgcacg ttgcccgccg cgtcgatcac
     6661 cgccaggtcg tgcagcccgt cgttcgccgg attgggggtc aactgcaacg cgcgcgtcgc
     6721 cagcccctcc accagcacct cgtcgccgac gaatacggtc agtttgccgc ccgcgtcctc
     6781 gagcgtccgc accggcagaa agttcgaaag ctgattgacg atctggtcgc gctggtcgag
     6841 cagggcattc cgcgcctcgt cggcccccgg cccggcgtcg tccagccggc ggttcacgtc
     6901 ggccagattc cgcgccagcg tgttgatctg ttccacctgc tggcggagct gcgcatcgag
     6961 ctgctggcgc tgctcgcgaa tctggccgcc caggccgcgc agcgaggcga caagctggct
     7021 cccccgcgag cgcaccgcct cgcgttcggc cggaccgccc ggcgtcacgc tcaagtcctg
     7081 cagtgccgcg aagaactgct cggtcgcctt ggagaacccc gagccgtcgg tgtcgcccag
     7141 cgctgtttcc agttgttgca gattgagcga aagcgtttgc aggcccccca tgcgggtgtt
     7201 gctctcgcgc agccgccccg cgaaaaagac gtcgcccagc cgctccacgc cgagcgactg
     7261 cacccccccc gtcgtgcgcg ccgcctgcat cagccgctgc cgcgagtagc ccggcgtgtt
     7321 gacgttggca atgttgttgc cggtcgtcgc cagcccgtgc tggctgacga agatgccggt
     7381 ccggccggtg ttgagaaccc caaacagcga catgtcggtt cgtgcctccc tggtccgcgg
     7441 gcccgctcag gcctgccgcg aaaagatgcc gccgccgccg ggcgcgaccg ggccgccgta
     7501 gccgcgggcg cgcggccgcg gcgcttcata cactttgccc gtcgggtcgg cctgctccag
     7561 cagcgtctga atcgccccgc ccaccagctc cagcccgcgg cgcgccgcgc gctcattgaa
     7621 ctgattgatt tcgcggcaac ggacgacgac ctcgcgcagc gccgccccga ccgcgcgcag
     7681 acggcccgcg atctccgccg gcgccgccgc gtacagctcg ctgatcgtca gttcgtgcgg
     7741 gggaagtccc agcgccgcgc cgagccgccg cgccagcacc cgccgcgatt cgtccagcgc
     7801 cttgatccgc agcgccagcg tctccttgcg cgccacgatc tcggccagcc ggtcgaccgc
     7861 catcgccagc attgcctcgc gttcctggtc gagcaggtcc gccatctctt cgtagatggc
     7921 gcgttcctcc ccgagcagcg tcagcaggga ctcgtaaggt tgttcggaca tgaaggcgtc
     7981 gccctgagcc gacgagggct gcacagcgac accggaaggc tgttgcggta cgaaatcagg
     8041 agggacggtc agcaggcgct cggggctttg cccagaatcg cctgggtcag ggcttcatcc
     8101 acgacgcggc gggccagctt ctcgctctcg acgtaatagc tgccctcttc gatcgcatcg
     8161 cgcaagccct cgaccttttc gagtcgaacc gtgggaacgg cctgcgccat ctggttcagc
     8221 cgctgcagtt cgcgggtgga ggacagctcc acgctgtcac ccgccgcggg gctcgccgtg
     8281 ggcgatgcct cgtcgacctg cggcgggccc gcaaatggtc ccagcggact gaccggattg
     8341 gacttacggc cttgaatctt catcgcttag ctcgccttga gggaatttcg aggggctgtg
     8401 gattcgccga accgctcaat cttgacccac attctccctt atcggcgctc ccttggcaaa
     8461 ctatagcatg ttttcccgcg cgacccgccg gacggttccc ttccgtgttt cgtagtccgc
     8521 accgcttgct tccggtgccg tcggggcatc tcctttcgtc ggaagatccg tcgccccgcc
     8581 ggccggttca ggcgccgccg gcgaggaaac tgttgccgtc ccgatcgctt cgggcgtttg
     8641 catcatctgc cggaccagcg cctggtgcag ccgcggatcc agtccccccg ccgcgccggc
     8701 tcgcgcgaat tcctggtcca gcatttccac atagtgcttg ccgccaagac ccttgcccag
     8761 caatcccccc tcgggcacgg tctggcgcat cgccttgacc atcatcgtca ggaaaaccgt
     8821 ctcgagctgc gtcgcgaccg cttcggcccg ttggcgcggg tcctcgatct ggttcagtcg
     8881 cgccagcgac tgcggatcat tcaggtcgag cgcccggccc ggatcgatga atgatccgca
     8941 gtcgctggcg cgactgaacc agatc
//
LOCUS       DAOPCO010000161         8896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7384_length_8896_cov_4.921502, whole genome shotgun sequence.
ACCESSION   DAOPCO010000161 DAOPCO010000000
VERSION     DAOPCO010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8896
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7384_length_8896_cov_4.921502"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1928
                     /locus_tag="PLS90_11295"
     CDS             <1..1928
                     /locus_tag="PLS90_11295"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPK03031.1"
                     /translation="VVLNGIGGMGLPHEMERVEANKPLAAALKARGIRRLLYVQTIGT
                     IFYEPFFDAVPGSEDWIQVNVDGVPWTYYDQWFRYIPCINNPEFIDYIRGVLQTAVRE
                     LDLDGVFTDNYGYYSYSCYCDHCKAAFRAYLNARYPDAEARQARFETAASFDHVVPPP
                     FRTLGARTMHTLINEEHQILDPVSQEWVRFRCERLGEVTRLLNEAIKEVNPDAVWFIN
                     YLYGGIPGLNLPVFHGSWAPDVYPHADLISAEASNYPRMENGVAQGRMMVMKIAKRFR
                     VPLSTVNFRALEDLTRISLAEAMAFNTAQVDLVGDIWHAGDLPQWMRDYHAFYREHQP
                     LTAGADTVADTAVLHSFETLAYTYLYPQESLVLAEQSLFQGGITYNVIFDDDLDHLDN
                     FRSLFLPNVLAMSRERAEQIADWVKRGGSLVVTEDTAILNQFMHPWRAEWLKARKTHL
                     LGELLGIDWPESGLVTQQVGAGRVAVIGRIERPWGEVRPITPTEHGLADVAMVQSRES
                     HLPWDYAEHWPTGPAIPLFMRAPALANNHDQIIEAVHYVLDGQRTVRVETDKPVIPEV
                     TRNANGTFVHLMNWEEREPVANFRVSVRTPGPVESVQLLSPDAGTPDTMLAAEYRDGR
                     TEFVVPRIECYNLVILK"
     gene            1939..3033
                     /locus_tag="PLS90_11300"
     CDS             1939..3033
                     /locus_tag="PLS90_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03032.1"
                     /translation="MKSHPGSLACWLLLAVGAPLAARADDPPPAAADESGASLPRESW
                     DGPLSVDHHECLLLDAVGVQMLAPAAPDDSLPVRRVEDLPLVVTEPCFVTTGDAAIRM
                     DREGCYRFLLHPDRTFQRILYLGDIPRFLGAIGNLQVHGWRDNGLTTEELIERAGRVC
                     ISITCGPIRDVALRLLDQQGIEARKVSTFTLDQWNSFDTGHALLEYRDPQTGDWILMD
                     PDLGHLFRADGELLDADRLTRAVRNGRPWDTLAVNAGPRVDMTPACPGGNPAVALYGL
                     RSALLTDPDQQRTWYRRVLQVTQLGDDYTVDTPAQRDRFEALCATANLDYTYLPPRAF
                     REKYYPARTDAALAADPSRPVHSPQTGKPE"
     gene            3030..3725
                     /locus_tag="PLS90_11305"
     CDS             3030..3725
                     /locus_tag="PLS90_11305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPK03033.1"
                     /translation="MTDHPAPACSPAIPGGAELNPDTTALVIVDMMNRFCDPAWLGRH
                     DDAAQVAWYRRELEGIIPRIRSVLESFRRRGALVVHVVLARWTPDAREVVPYQRGREY
                     DAFDTQAMSVIPELAPATGEIVIRKVASSAFQATGLDFMLRNAGIRQVVITGQFGDAC
                     CFYTLIMSREAGFANIWVHDALLFSNEQMKQTINPLVAGRWAESLSAEELVRRLEPSR
                     AAAAPIPSLARKA"
     gene            3746..4975
                     /locus_tag="PLS90_11310"
     CDS             3746..4975
                     /locus_tag="PLS90_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK03034.1"
                     /translation="MARYGAKAAAADSGVLPTPFPRTIGPNALMYLQEVVDSGLTVDM
                     TDRFEQAFARAMGVAHCIATSGCTPSLHALVLASAWEPGDEVIVSPITDYGTVQGLIA
                     QGIIPVFPDTEPGSINFSARTIQPHLTDRTRAIFCVHKTGLMVDMDPLLELAARRGIP
                     VYEDVCQAIFSVYKGRTAGTLGHAAAFSFDPEKTMGSDVGGCVITNDDALAERIRFVG
                     QARGGQMRPGFGRVHTVAGYAYRMPQCTAAICLAQLEIIREQVAHIDAMIRLQTDLLR
                     QIPGIRPLAIPEYQDVYSCWMAGISLEPEAFNCSAGEFAAQLAEAGLPGAGLGKYYLM
                     PAALECLRERADEQRYPYSMPPASRRFSYRGEDLPNAWGFLETFIRWSTFCAKYQPDD
                     CRRAAEIVREVAERNRR"
     gene            5245..6384
                     /locus_tag="PLS90_11315"
     CDS             5245..6384
                     /locus_tag="PLS90_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK03035.1"
                     /translation="MAKQKPAARATSDGQAENALRSRFRSITIKDVAQRAGVSRITAA
                     RILSGNPNYPASPETVEKVRNAAREMNYQPNPFAQSLGRRRSHIVGLCVHQDATVGER
                     VSHSEYLVHLDPHIAGIHSHPEAARYSVMVIRRDDADPELEANLRQKLRYLDGLIYVT
                     PRQLHLPILTRIADSMPLVLENAPDVKGINTVSVDEHGIIADAVRLLVQRGCRKLGLI
                     TWGAQDICANVSRSRAFRKALKSLGLPLSDEQVALGNSRFQTRGVMRDMLQRVPDLDG
                     IIVPRDSALSNAIEELRLCGRQVGRDLAIISIVETSLCSLLQPSITAIHFPFDLLANR
                     AFDLLLQTIEGKLTRSKRLLIPAVINERDSTRLFSEARARRLVAT"
     gene            6860..7714
                     /locus_tag="PLS90_11320"
     CDS             6860..7714
                     /locus_tag="PLS90_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPK03036.1"
                     /translation="MVRQPAEQPQRAFWRDQMEQAYRFMMQILDYPVQESLEPLVSIP
                     DALRGQQVEILYAEQNSSRPYTPIFALREGLIKDFVAIARALNEQGLVLRIEDAYRTP
                     AMQRKLALGMFDTLLQQTLWECNGNVPSADLVLRRLTVLVATIPKIGTHISGSALDVS
                     VVRMDDGAELDRGAPYLELSPLTPMDSPFIPVDAQHTRQRITEAFLRGGFLPYPFEFW
                     HFSKGDAYEHHLRQSGQPAKYGAVEWDPRTGRVTALDNPRQSLISVEDVQLELEQAFE
                     RLGILASE"
     gene            7947..8675
                     /locus_tag="PLS90_11325"
     CDS             7947..8675
                     /locus_tag="PLS90_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03037.1"
                     /translation="MLSAVFAILILAPATGWCQYGYGENLLINPSFEDDTLSRGIPDG
                     VKYSGRNGIPASTYRGQFFKDDAVAHTGASSLRLDLPTSNTWARTNPGFYGNADGSET
                     ILNPQLDAGTLGWKLRLKFWARDNGAAKPGEFQGPRLFQHPDEESGGSLAGFTGQMSA
                     NAATYVVDWGNINATIDTTWQEYWYEFNVVDNTHSYSVEWALSDYPNARAAVYSLYID
                     DVELYYVDADGQPVPAELSSFVLE"
BASE COUNT         1514 a         3216 c         2631 g         1535 t
ORIGIN      
        1 tcgtcgtcct caacggcatc ggcggcatgg gcctcccgca cgagatggag cgggtcgagg
       61 cgaacaagcc gctggccgcc gcgctcaagg ccaggggcat ccggcgcctg ctctacgtgc
      121 agaccatcgg cacgatcttc tatgaaccgt tcttcgatgc cgtccccggc tccgaagact
      181 ggatccaggt caacgtcgac ggcgtccctt ggacctatta cgatcaatgg ttccgctaca
      241 tcccctgcat caacaacccc gaattcattg actatattcg cggcgtgctg caaacggccg
      301 tccgcgagct cgacctcgac ggcgtcttca ccgacaacta cggctactac agctactcgt
      361 gctactgcga ccactgcaag gcggcctttc gggcgtatct caacgcgcgc taccccgacg
      421 ccgaggcgcg ccaggcccgc tttgaaaccg ccgcttcctt cgaccacgtc gtgccgccgc
      481 cgttccgcac gctcggcgcg cgcacgatgc acacgctgat caacgaggaa catcagatcc
      541 tcgatccggt ctcgcaggag tgggtgcggt tccgctgcga acggctcggc gaggtcaccc
      601 gcctgctcaa cgaagcgatc aaggaggtca atcccgacgc ggtctggttc atcaactacc
      661 tctatggcgg catccccggg ttgaatctgc cggtgttcca tggttcatgg gccccggacg
      721 tctatcccca cgccgatctg atctcggccg aggcctccaa ttatccgcgc atggagaacg
      781 gcgtcgcgca ggggcggatg atggtgatga aaatcgccaa acgcttccgc gtcccgctct
      841 cgacggtcaa cttccgcgcg ctggaggatc tgacgcggat ctcgctggcc gaagcgatgg
      901 cctttaacac ggcccaggtc gatctggtgg gcgacatctg gcacgccggc gacctgccgc
      961 agtggatgcg cgattaccac gccttctatc gcgagcatca gccgctgacg gcgggggccg
     1021 ataccgtcgc cgacaccgcc gtgctccatt cgttcgaaac gctcgcctac acttatcttt
     1081 atccccagga aagcctggtc ctcgccgagc aatcgctctt ccagggcggc attacctaca
     1141 acgtcatctt cgacgacgat ctggaccacc tggacaactt ccgcagcctg ttcctgccca
     1201 acgtgctggc gatgagccgt gagcgcgccg aacagatcgc cgattgggtc aagcgcggcg
     1261 gctcgctggt ggtcaccgag gacacagcca tcctgaatca gttcatgcac ccgtggcgcg
     1321 ccgagtggct caaggcccgc aaaactcatc tgctgggcga gctgctgggg attgactggc
     1381 ccgagagcgg tctggtgaca caacaggtcg gcgccggccg cgtcgccgtc atcggccgga
     1441 tcgaacgccc gtggggtgag gtgcggccga tcacgccgac ggaacatggc ctcgccgatg
     1501 tcgcgatggt ccagtcgcgc gaatcgcacc tgccctggga ctatgccgag cactggccga
     1561 cgggcccggc gattccgttg tttatgcgcg cccccgcgct cgcgaacaat cacgaccaga
     1621 tcatcgaggc cgtccactac gttctggacg ggcaacgcac cgtgcgcgtc gaaacggaca
     1681 agccggtgat ccccgaggtg acgcgcaatg ccaacgggac gtttgttcac ctgatgaact
     1741 gggaggagcg cgagcccgtc gccaacttcc gcgtctcggt gcggacgccc ggcccggtcg
     1801 agtcggtcca gcttctctcg cccgacgccg gaacgccaga caccatgctg gcggcggagt
     1861 atcgcgacgg ccggacggaa ttcgtcgtgc cgcgcattga atgttataac ctcgtgattc
     1921 tgaagtaacg gtgaccccat gaaatcgcat cctggttctc ttgcctgctg gctgctgctg
     1981 gccgtcggcg cgccgctggc cgcgcgggct gacgatccgc cccccgccgc cgccgatgaa
     2041 tcgggcgcct ccctgccgcg cgagtcgtgg gacgggccgc tctcggtcga ccaccacgaa
     2101 tgcctgctgc ttgacgcggt cggggtccag atgctggcgc ccgcggcgcc cgacgactcg
     2161 ctgccggttc gccgcgtcga ggacctgccc ctcgtcgtca ccgaaccgtg cttcgtcacg
     2221 accggcgacg ccgcgatccg gatggatcgc gagggctgct accgcttcct cctgcacccc
     2281 gatcgcacct tccagcgcat cctttatctg ggcgatatcc cccgctttct gggcgcgatc
     2341 ggcaatctgc aggttcatgg ctggcgcgac aacggcctga cgaccgagga gctgatcgaa
     2401 cgggccgggc gcgtctgcat ctcgatcacc tgcggcccga tccgcgacgt cgcgctgcgc
     2461 ctgcttgatc aacaggggat cgaggcgcgc aaggtcagca cgttcaccct ggaccagtgg
     2521 aactcgttcg acaccggcca cgcgctgctg gaataccgcg acccgcaaac gggcgactgg
     2581 atcctgatgg atcccgatct gggccatctc ttccgcgccg acggcgaact gctcgacgcc
     2641 gaccggctga cgcgcgccgt ccgcaatggc cggccgtggg atacgctggc cgtcaatgcc
     2701 ggaccccggg tcgatatgac gccggcctgc ccggggggca accccgccgt cgcgctctac
     2761 ggcctgcgct ccgcgctctt gactgatccc gaccagcaac ggacatggta ccgccgcgtg
     2821 ctgcaggtca cgcaactggg cgacgactac accgtcgata cgccggcgca gcgggatcgc
     2881 ttcgaagcgc tctgcgccac ggccaatctc gactacacct acctgccccc gcgggcgttc
     2941 cgcgaaaaat actacccggc gcggaccgac gcggcgcttg ccgccgaccc gtcccggccg
     3001 gtgcactctc cccagacagg aaaaccagaa tgaccgatca tccagcaccc gcctgttcgc
     3061 ccgcgatccc gggcggcgcg gaactcaacc ccgacacgac ggcgctcgtc atcgtcgaca
     3121 tgatgaaccg cttctgcgac ccggcctggc ttggccgcca cgacgacgcc gcccaggtcg
     3181 cctggtatcg ccgcgagctg gaaggaatca tcccgcggat caggtcggtg ctggaaagct
     3241 tccgccgccg cggcgccctg gtcgtgcatg tcgtgctcgc ccgctggacg cccgacgcgc
     3301 gcgaagtcgt cccctaccag cgcggccgcg aatacgacgc cttcgatacc caggcgatgt
     3361 cggtcattcc cgagctggcg cccgcgaccg gcgagatcgt gatccgcaag gtcgccagct
     3421 cggcatttca ggcgaccggc ctcgatttca tgctgcgcaa cgccggcatc cgccaggtcg
     3481 tgatcaccgg ccagttcggc gatgcctgct gcttctatac gctgatcatg agccgcgagg
     3541 ccgggttcgc caacatctgg gtccatgacg ccctgctctt ctccaacgaa cagatgaagc
     3601 agacgatcaa tccgctggtg gccggccggt gggccgagtc gctctcggcc gaagaactcg
     3661 tccgccgcct ggagccgtcg cgcgcggccg ccgcgccaat cccatcactc gcccgaaagg
     3721 catgaccgtg acaccgaaga ttgaaatggc tcgttacggc gcgaaagccg ccgccgccga
     3781 ttccggggtg ctccccaccc cgttcccacg cacgatcggc cccaacgcgc tcatgtacct
     3841 tcaggaagtc gtcgactccg gcctgacggt cgacatgacc gaccggttcg aacaggcgtt
     3901 cgccagggcg atgggcgtcg cccattgcat cgctacgtcg ggctgcacgc cgagcctcca
     3961 cgcgctggtg ctcgcctcgg cctgggagcc cggcgacgaa gtgattgtca gcccgatcac
     4021 cgattacggc accgtccagg gcctgatcgc gcaggggatc atcccggtct tccccgacac
     4081 cgagcccggt tcgatcaact tctccgcccg gacgattcaa ccgcacctga ccgaccgcac
     4141 gcgcgccatc ttctgcgttc acaaaacggg actgatggtc gatatggacc cgctgctgga
     4201 actcgccgcg cgccgcggca tcccggtcta cgaggatgtc tgccaggcga tcttcagcgt
     4261 ctacaagggc cggacggccg gaacgctcgg gcacgcggcg gccttctcgt tcgaccccga
     4321 aaaaacgatg ggctccgacg tcggcggctg cgtcatcacc aacgacgacg cgctggccga
     4381 gcgcatccgc ttcgtcggcc aggcccgcgg cggccagatg cgacccggct tcggccgcgt
     4441 ccataccgtc gccggctatg cctaccgcat gccgcagtgc acggcggcga tctgcctggc
     4501 gcagctcgaa atcatccgcg agcaggtggc ccacatcgat gcgatgatcc gcctgcaaac
     4561 cgatctgctg cggcagatcc cgggcatccg cccgctcgcc attccggagt atcaggatgt
     4621 ctactcgtgc tggatggccg gaatcagcct cgagccggaa gctttcaact gctcggccgg
     4681 cgaattcgcc gcccaactgg ccgaagcggg cctgcccggc gccgggctcg gcaaatatta
     4741 cctgatgccc gcggcgctgg aatgtctgcg ggagcgcgcc gacgagcagc gctaccccta
     4801 ttcgatgccg ccggcctcgc gccggttcag ctatcgcggc gaggatctgc ccaacgcctg
     4861 gggtttcctt gagaccttca tccgctggtc gaccttctgc gccaagtacc aaccggatga
     4921 ttgccggcgg gcggctgaga tcgtgcgcga ggtcgccgaa cgcaatcgcc gctgaccccc
     4981 gcaaatcaat gcggccggcc gcgtgcgtaa cttgtcgcaa ccggcgaagc gctcggcttc
     5041 gacggatctc cgcccgcgct ccctcgcgcg gcggacgatg ccccgtccag cgccattcgc
     5101 cccgcgcgta caacccaaat cgtgttgaca accgccgccg gttaagggga atttggaatt
     5161 gagttgggcc ttgcccgctc gccggcctga gccctcgccg caccgccgat tctcggctta
     5221 ccagaccgtt aggatccatc atttatggcg aagcagaaac cagccgcgcg cgcaacctcc
     5281 gacgggcagg ccgaaaacgc tctccggagc cggtttcgct cgataaccat caaggatgtc
     5341 gcgcagcgcg ccggcgtcag ccgcatcacc gccgcgcgca tcctgtcggg caatcccaac
     5401 tatccggcgt cgcccgaaac cgtcgagaag gtccgcaacg ccgcgcgcga aatgaactat
     5461 cagcccaacc cgttcgccca gtcgctcggc cgccgccgca gccacatcgt cggcttgtgc
     5521 gtgcaccagg acgcgaccgt cggcgaacgc gtctctcact ccgaatacct cgttcacctc
     5581 gatccgcaca tcgccgggat tcactcgcat cccgaagccg cgcgctactc ggtgatggtc
     5641 atccgccgcg acgacgccga cccggagctc gaagcgaacc tgcgccagaa actgcggtat
     5701 ctcgacggct tgatctacgt cacgccgcgc cagttgcatc tgccgatcct gacccgcatc
     5761 gccgactcga tgccgctcgt cctcgagaac gcccccgacg tcaagggcat caacaccgtc
     5821 tcggtcgacg agcatgggat catcgccgac gccgtccgcc tgctcgtcca gcgcggttgc
     5881 cgcaaactgg ggctgatcac ctggggcgcg caggacatct gcgccaacgt cagccgctcg
     5941 cgggcgttcc gcaaagcgct caagtcgctc gggctcccgc tcagcgacga gcaggtcgcc
     6001 ctgggcaaca gccgcttcca gacgcgcggc gtgatgcgcg acatgctcca gcgcgtcccc
     6061 gatctggacg gcatcatcgt gccgcgcgac tcggcgctga gcaatgccat cgaggaattg
     6121 cgcctctgcg gccgccaggt cggccgcgac ctggcgatca tttccatcgt cgagacctcc
     6181 ctgtgcagcc tgctccagcc gagcatcacc gcgatccact tcccgttcga tctgctcgcc
     6241 aaccgggcct tcgatctgct gctccagacg atcgagggca aactgacccg gagcaagcgc
     6301 ctgctgatcc cggcggtcat caacgagcgc gattccacgc gcctgttctc cgaagcccgc
     6361 gcgcgccggc tcgtggcgac ctgaccagga tactcgtccc ccgtatctcg ccctcgtcct
     6421 cgtaatcgcc cccaacgccc ccccagattt cggctctcgg ctctcgtccc tcgtcctcgg
     6481 ctctcgtatg aaacgccgag caacgagcaa ctcgtcccat cctcatcatc ctcgtccgtc
     6541 ttctcccccc tcctcccgcc ccccgatcct ccaactttcc cccctgcggt tttcctcgta
     6601 atcaccaatg acaaacttgc aaaaatgaca taaatgacgt cattgacgag acggcttgga
     6661 gcgccgagcc tgatccccgg agggccgagc ctgatactcg attggacggc cgggcctgat
     6721 actcggatat attttttgct tgacgccgcg cacccgcctc cgctacgctt accccatcga
     6781 cggttttaca tccttgtgat acccggatat cgccaagggt taactggcgc gggttcaccc
     6841 caaagggcgg ttttctagca tggtgcggca accggccgag cagccccagc gggccttctg
     6901 gcgcgatcag atggagcaag cctaccggtt catgatgcag atcctggact accccgttca
     6961 ggaatcgctc gagccgctcg tctccatccc cgacgccctg cgcggtcagc aggtcgagat
     7021 cctctacgcc gaacaaaaca gctcccgtcc ctacaccccg atctttgccc tgcgcgaggg
     7081 cctgatcaag gacttcgtcg ccatcgcccg cgcgctcaac gagcaggggc tcgtcctgcg
     7141 catcgaggac gcctaccgca ccccggccat gcagcgcaag ctcgccctcg gcatgtttga
     7201 caccctcctc cagcagacgc tctgggaatg caacggcaac gtcccctcgg ccgacctcgt
     7261 cctgcgccgg ctgaccgtgc tggtcgcgac gatccccaag atcgggacgc acatctcggg
     7321 cagcgccctg gatgtttcgg tcgtccgcat ggacgacggc gccgaactcg atcgcggcgc
     7381 gccctacctg gaactctcgc cgctcacgcc gatggattcg ccgttcatcc cggtcgacgc
     7441 ccagcacacg cgccagcgga tcaccgaggc gttcctgcgc ggcggctttc tcccctaccc
     7501 ctttgaattc tggcacttca gcaaggggga cgcctatgaa caccatctgc gccagtccgg
     7561 tcagccggcc aagtacggcg cggtggagtg ggatccgcgg accgggcgcg tgacggcctt
     7621 ggacaatccc aggcaatcgc tgatttcggt cgaagatgtg caactggagc tcgaacaggc
     7681 gttcgagcgt ctgggcatcc tggcgagcga gtagtccgcg ccgcactctg gaaaggaggt
     7741 ataaccagta gcgcgatact ttgtccgcgg acggggtggt cgccggcggc tcggctgtct
     7801 ctccccacgg agcccctcgc gctccccgcc cgcgccagtg tagctcaggt gccttttact
     7861 catgcgggga acatgtttcg gcctgactac tgcgtcgacc atctctttga aagcctggag
     7921 gggctatgat gagaaaactc attcccttgc tgtctgccgt attcgccatc ctgatccttg
     7981 ccccggcaac cggctggtgc cagtatggct acggcgaaaa cctgttgatc aatccgtcct
     8041 tcgaggacga cacgctctcg cgcgggatcc ccgacggcgt caaatacagc ggccgcaacg
     8101 gcatccccgc ttccacctat cgcgggcagt tcttcaagga cgacgcggtg gcccataccg
     8161 gcgccagttc gctgcgactc gatctgccca cctcgaacac gtgggcccgc accaacccgg
     8221 gcttctacgg caacgccgac ggctccgaaa cgatcctcaa cccgcaactc gacgccggca
     8281 cgctcggctg gaagctccgc ctgaagttct gggcccggga taacggcgcc gccaagcccg
     8341 gtgagttcca gggaccgcgg ctgttccaac acccggacga agagtccggc ggctcgcttg
     8401 ccggcttcac cggtcagatg agcgccaatg ccgcgaccta cgtcgtggat tggggtaaca
     8461 tcaacgccac catcgacacc acctggcagg aatactggta cgagttcaac gtcgtcgaca
     8521 atacgcactc gtactccgtt gagtgggcgc tgtcggacta tcccaatgcg cgcgccgcgg
     8581 tctacagcct ctacatcgac gacgtggagc tgtactacgt tgacgccgac ggtcagccgg
     8641 tccccgccga gctgtcgtcg ttcgttctgg aataacgacg ggcaaatctc ggcggagccg
     8701 gccgcggccg gcccgctgga actggccaga tccacggcgc caggtttttg ccgtggatct
     8761 ggcccccagc cctctcgagg agtttgaacc cctcatccca acgcatggag aaccggctca
     8821 tgaagcacag actattttct ttgttgtgga gcgcgctgct cctgacgggc ctggcgctgc
     8881 tggcgccgcg cgcgga
//
LOCUS       DAOPCO010000162         8876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7399_length_8876_cov_5.080036, whole genome shotgun sequence.
ACCESSION   DAOPCO010000162 DAOPCO010000000
VERSION     DAOPCO010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7399_length_8876_cov_5.080036"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLS90_11330"
     CDS             <1..807
                     /locus_tag="PLS90_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPK03038.1"
                     /translation="EQALAATETVIAVDIFPSWITRHADIVLPGSLFYENDGTMTDID
                     GTLQRMSRGDRPRGDAQEEWRILESIAVLMSAARRHKSAQSIFGDLLKAWNAPTLFRL
                     DDLLLDPPSLLPQTNHVLLSRRTRPNFKLILAERGAQPPAQAAPAAGGGLHLLWNMHS
                     QGPDHLGSRSSEFDALRPQPRLELNPADAAALGLADGDWVRLAGGGARPSQIAVSRFV
                     APGVAAGAANVLGLRLPADEVYLPVIQLERAIGPLPDEALYQEEAVETPA"
     gene            804..2060
                     /gene="nuoH"
                     /locus_tag="PLS90_11335"
     CDS             804..2060
                     /gene="nuoH"
                     /locus_tag="PLS90_11335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HPK03039.1"
                     /translation="MTLTLTPFAIEMLLRLVLILVIFHVLLIIVPFMVLWERKLLGFI
                     QERPGPNRVGPWGILQTIVDGLKLFLKEDIRPASVDRTLHTLAPLMTVAPAILVLAIV
                     PFADPVRITLDGGEFSLLGLTIQVAPVVLDLPIGITSLEVGILYYLAITGLAVYGIVL
                     AGWASNSKYSLLGGIRSSAQMLSYEIALGLSLVGVLLIAGTFDLRAIIEQQDGGFWNW
                     HVWKQPVGFLLFMVAGFAETNRLPFDLPEGESELGAGFHTEFSSMKFAMFFMAEYMNM
                     FTFSAVLATLFLGGYTGPIASPFAELGWLNVAWGVFWLMAKIFLIVSFMIFIRGTLPR
                     FRYDQLMDLGWKVMFPLALANMVLTAGVVAVFPPGNRWVTREEIIGQPSTVGLTLGLL
                     VVGVAQIIIMDMILNARKRRLLANAR"
     gene            2050..2517
                     /gene="nuoI"
                     /locus_tag="PLS90_11340"
     CDS             2050..2517
                     /gene="nuoI"
                     /locus_tag="PLS90_11340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HPK03040.1"
                     /translation="MLVDLFKGFAVTFKHIFRPRVTLEYPEVKRPLPPYFRGRHRLQR
                     YENGLERCVGCALCAAVCPSEAIYLEAAENDPANPVSAGERYARVYQIHQLRCIWCGF
                     CEEACPEDAIVMGPDFELANYRRDQFICDKQAMLDPPDKGFGKEPNLKVNTAL"
     gene            2534..3079
                     /locus_tag="PLS90_11345"
     CDS             2534..3079
                     /locus_tag="PLS90_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HPK03041.1"
                     /translation="MISQIVYGLLFVLALFSAMMVVLSPHAVYGALYLVLTMVGIAAL
                     FVLMNAQLAGVLQIIVYAGAIMVLFLFVIMLLNVGRQEGGSGINRSVRWLGGVLSVAF
                     ILQVIAMYAGLRDELAANYTLEGAQSIRIDDVARLLLTDYIYAFEMTSVLLLVAVIGA
                     VVLARRRLVQGALEEGERRGF"
     gene            3191..3487
                     /gene="nuoK"
                     /locus_tag="PLS90_11350"
     CDS             3191..3487
                     /gene="nuoK"
                     /locus_tag="PLS90_11350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HPK03042.1"
                     /translation="MVPGNLILAASIFTIGVFGILLKRNAISIFMCVELMFNAANLAF
                     ITFAQSLGRPEGVAVFLFVITVAAAEAAVGLALFIKIFNDKETIEADTLDLLQR"
     gene            3554..5032
                     /locus_tag="PLS90_11355"
     CDS             3554..5032
                     /locus_tag="PLS90_11355"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HPK03043.1"
                     /translation="MNDLYPQISASLFQTLPILVLIVAGIGVLVADVLLPRASRWLLS
                     WLSLLGVALAFIALDFVVGPAGREALPLHSYFGVLRSNYLVFLAQNVILAAGAFVILI
                     SPRYVGERRIPLGEYYGLLLLAVMAMLALAASTELLTVFLNLELLSITLYVLTGIENR
                     NLRSGEAAFKYFLLGSFAAAFLLLGIAFVFGATGETRYDAIRQVLVDGTAQPRLLAIG
                     FCLMLVGFGFKLSLAPFHMYAPDVYEGAPTTTASLIATGTKVAGFAALFHLIELAAAW
                     SDAAGGLWLALYAVALASMIVGNVGAVVQPNIKRMLAYSSIAHSGYTIVPMVVMLQRP
                     DLIADARDAVAYYMLAYTVMTLLAFGVPAVLGPAGESRIAQYAGLHRRSPMLACVMAL
                     AMVSLMGIPPTIGFFGKFQLFAVAVQGDHIWLAVLGVLASVASVYYYLRVIVTMYMEE
                     PATAGEELESRRLDGVNAFALTACSVCVFLFVVFQSLYLFGV"
     gene            5074..7026
                     /gene="nuoL"
                     /locus_tag="PLS90_11360"
     CDS             5074..7026
                     /gene="nuoL"
                     /locus_tag="PLS90_11360"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HPK03044.1"
                     /translation="MSSIWIILIPLLPLLAFVANGLFGRALQRTGGWLAVGCMGGSAL
                     IGAVIFFGGLFGGITFPIQVELWQWMPLGDVTIRIGFYVDQLTALMLFFVTFVSTLVF
                     LYSMGYMAGDPGFNRFFAYLGLFDFSMLVLVLGDSLPVLFVGWEGVGLCSYLLIGYYL
                     AMPGAPDAGKKAFIVNRIGDFGFLVAMFMLFASVGTLNIQEILAAVASGVVPEFQYGA
                     AFVTFLTLMLFLGCTGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVTAGVYLVVRLS
                     ALFALAPATMLTIAIVAAATAFVAGSIALTQKDIKKVLAYSTVSQLGYMFLACGVGAF
                     TAGIFHVFTHAFFKGCLFLGAGSVIHACHHIQDMDQMGGLRRKMPATARTFLFGSLGL
                     AGIVPFAGFMSKDEILWRVFSDGKFILYAIALVTAAFTAYYAFRAYFLTFEGQSRLPH
                     DVEHHVHESPWTMTLPLWILGVGALGAGVFNILPAFGHGHYSAINHFLAPVVAPAEAI
                     LAAHASHEPIHSFALEILLMIVSTVVALVCGVGLAWLFAYKLWPAGASRMARRLGPLY
                     RISYHRWWWDEFYNVFVAGGLKLLALISTLFDRFVIDGVLHTVAGAARSGSRLIRFFQ
                     NGQVQAYALAILVGVNLVVWLVLWYQ"
     gene            7116..8642
                     /locus_tag="PLS90_11365"
     CDS             7116..8642
                     /locus_tag="PLS90_11365"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPK03045.1"
                     /translation="MPELTNHHAALLLLMTFLPAVTGIVIAAAVPAALARTVALAGAV
                     LTFLVSLTFVGGIFGMPADFTAAVNIPWIERYNVALLMGLDGLSGSLVLLTNVLIILA
                     VLGSWTAIAARQKEFYFYLLLLQTGILGAFLALDLLLFYIFWEIMLIPLYFLIGIFGS
                     KNRIYATTKFFLYTMAGSLLMLLGIIALYFHTGSFALADALGAAATLDPTARLLIFLS
                     FVIAFAIKVPLFPLHTWLPDAHTEAPTAGSVVLAGVLLKTGVYGLLRFAIPLFPAEAA
                     RIAPLMMLLAVIAIIYGALTAFAQRDMKRLVAYSSVSHMGFIVLGVFAFREAAITGAS
                     LQMINHGISTSGLFLCVGYLYERRHTRELAAFGGLAHNMKLYAVLTMIMILSSVGLPG
                     LNGFVGEFMILVGAYPANAVLTIIAATGVILAACYLLRLMQETFYGPLDKDENKVLRD
                     LNPRESFTLVVLAVFALWIGLYPRTFTEPLAPVSERIVRAVEAAQSPGTALADATAGK
                     "
BASE COUNT         1257 a         3014 c         2776 g         1829 t
ORIGIN      
        1 gagcaggcgc tcgcggcgac cgagaccgtc atcgccgtcg atatcttccc ctcctggatc
       61 acgcgccacg ccgacatcgt actgcccgga tcgctgttct acgagaacga cggcacaatg
      121 accgacatcg acggcacgct gcagcgcatg agccgagggg atcgcccgcg cggcgacgcg
      181 caggaggagt ggcggattct ggagtcgatc gccgtgctga tgtcggcggc gcggcgtcac
      241 aagagcgccc agtcgatctt cggcgatctg ctgaaagcct ggaacgcgcc gacgctgttc
      301 cggctcgacg acctgctgct cgatccgccg tcgctgctgc cgcagaccaa tcacgtcctg
      361 ctctcccggc ggacgcggcc caacttcaag ctgatcctgg ccgagcgcgg cgcgcaaccg
      421 ccggcacagg ccgccccggc cgctggcggc gggctgcatc tgctctggaa catgcatagc
      481 caggggcccg atcatctcgg ctcccgctcg tcggaattcg acgcactgcg cccgcagcca
      541 cggctggaac tcaacccggc ggacgccgcg gcgctcggcc tggccgacgg cgactgggtc
      601 cgtctcgccg gcggtggcgc ccgccccagc cagatcgccg tcagccggtt cgtggcgccg
      661 ggcgtcgccg ccggcgcggc caacgtcctg gggctgcgtc tgccggccga cgaggtttat
      721 ctgcccgtga tccagcttga acgcgccatc ggcccgttgc ccgatgaagc cctgtatcag
      781 gaagaagccg tggagacgcc ggcatgacgc tgacccttac tccgtttgcc atcgagatgc
      841 tcctgcggct ggtgctcatc ctggtcattt tccatgtgct gctgatcatc gtcccgttta
      901 tggtgctgtg ggagcgcaag ctgctcggct tcattcagga gcggccgggg cccaatcgcg
      961 tcggtccgtg ggggattctg cagacgatcg tcgacggcct gaagctcttc ctgaaggaag
     1021 acatccggcc ggcctcggtc gatcgcacgc tccatacgct ggcgccgctg atgaccgtgg
     1081 cgccggcgat cctggtgctg gccatcgttc cgttcgccga tccggtgcgg atcaccctcg
     1141 atggcggcga gttctcgctc ctcgggctca ctattcaggt ggcgcccgtc gtcctcgacc
     1201 tcccgatcgg catcaccagt ctcgaagtcg gcatcctcta ttatctggcg atcaccggcc
     1261 tggcggtcta cggcatcgtg ctggccgggt gggcgtccaa cagcaagtat tcgctgctgg
     1321 ggggcatccg ttcgagcgcc cagatgctga gttatgagat cgccttgggg ctctcgctgg
     1381 tcggcgtgct gctgatcgcc ggcacgtttg atctgcgcgc gatcatcgag cagcaggacg
     1441 gcggcttctg gaactggcac gtctggaaac agccggtcgg cttcctgctg ttcatggtcg
     1501 ccggcttcgc cgagacgaac cgcctgccgt tcgacctgcc cgagggcgag agcgaactgg
     1561 gcgcgggctt ccacaccgaa ttcagctcga tgaagttcgc catgttcttc atggcggaat
     1621 acatgaacat gttcacgttc tcggcggtgc tggcgacgct gttcctgggc ggctacaccg
     1681 gtccgatcgc gagcccgttc gccgagctgg gctggctcaa cgtcgcctgg ggggtgttct
     1741 ggctgatggc caagatcttc ctgatcgtca gcttcatgat cttcatccgc ggcacgctcc
     1801 cccggttccg ctacgatcag ctcatggatc tgggctggaa ggtgatgttt ccgctcgcgc
     1861 tggccaacat ggtgctgacg gcgggggtcg tggcggtgtt cccccccggc aaccgctggg
     1921 tcacgcgcga ggagatcatc ggtcagccgt cgacggtggg gctgacgctc gggctgctgg
     1981 tggtcggcgt cgcgcaaatc atcatcatgg acatgatcct taacgccagg aagaggcggc
     2041 tgctcgcaaa tgctcgttga tctgttcaaa ggcttcgccg tcacgttcaa gcacatcttc
     2101 cggccgcggg tgacgctgga gtatccggaa gtgaagcggc cgttgccgcc ctacttccgc
     2161 gggcgccacc gcctgcagcg ctacgagaac gggctggagc gctgcgtcgg ctgcgcgctg
     2221 tgcgcggccg tctgcccgtc ggaggcgatc tatctggaag cggccgagaa cgatccggcc
     2281 aatccggtct cggccggcga acgctacgcc cgcgtctatc agatccatca gttgcgctgc
     2341 atctggtgcg ggttctgcga ggaggcctgc cccgaggatg cgatcgtgat gggtccggac
     2401 ttcgaactgg ccaactatcg gcgcgaccag ttcatctgcg acaagcaagc gatgctcgac
     2461 ccgcccgaca agggcttcgg caaggaaccg aatctgaagg tgaacacggc gctgtagcgg
     2521 aggggaggga gcggtgatct cacaaatcgt ctacggcctg ctgttcgtgc tggcgctctt
     2581 ttcggcgatg atggtcgtgc tgtcgcccca cgcggtctac ggcgcgctct acctggtgtt
     2641 gacgatggtg ggcattgccg cgctgttcgt gctgatgaac gcgcagctgg ccggcgtgct
     2701 gcagatcatc gtctacgccg gcgcgatcat ggtcctgttt ctgttcgtca tcatgctgct
     2761 caacgtcggg cgccaggagg ggggctcggg catcaaccgg tcggtccgct ggctgggcgg
     2821 cgtgctgtcg gtcgcgttca tcctgcaggt gattgcgatg tatgccggcc tgcgcgacga
     2881 gctggcggcg aactacacgc tggagggggc ccagagcatc cgcatcgacg acgtcgcccg
     2941 gctgctgctg accgattaca tctacgcctt cgagatgacc tcggtgctgt tgctggtggc
     3001 ggtgatcggc gccgtggtgc tggcccggcg gcgactcgtg cagggagcgc tggaggaagg
     3061 ggagcgaagg gggttctaga gaagttgatc gtctcccgtt ttgcttcgtc ctcgtcatcg
     3121 aaggagcgtt cactaaagcc aatgatgtct aagtttgatc acgaccgact ggtttaaccc
     3181 aatggaattc atggtccccg gcaatctgat tctggccgcg tcgatcttca cgatcggcgt
     3241 gttcggcatc ctgctcaagc gcaacgccat ttcgatcttc atgtgcgtcg agctgatgtt
     3301 caacgccgcc aacctggcgt tcatcacgtt tgcccagagc ctgggtaggc ccgaaggggt
     3361 cgccgtgttt ctgttcgtca tcacggtggc ggcggccgag gcggccgtcg ggctcgcgct
     3421 gttcatcaag atcttcaacg acaaggagac gatcgaggcc gacacgctgg atctgctcca
     3481 gcgctgaagc ttttgcgatc ccgcgccgcg catgccgccg gcgctgtaac gaccgacgga
     3541 taacaaactg ctggtgaacg acctctaccc ccagatctcc gcgtcgctgt tccagacgct
     3601 gccgatcctc gtgctgatcg tcgcgggcat cggcgtgctg gtcgccgacg tgctgctgcc
     3661 ccgcgccagc cgctggctcc tgtcgtggct gagcctgctg ggcgtcgccc tcgccttcat
     3721 cgcgctggac ttcgtcgtcg gccccgccgg gcgcgaggct ttgcccctgc atagttactt
     3781 cggcgtgctg cgctcgaact atctcgtctt cctggcgcag aacgtgatcc tggccgccgg
     3841 ggcattcgtc atcctgatct cgccgcgcta cgtcggcgag cgccgcatcc cgctgggcga
     3901 gtattacggc ctgctgctgc tggcggtgat ggcgatgctc gcgctcgccg cctcgaccga
     3961 actgctgacc gtcttcctca acctcgaact gctctcgatc acgctgtatg tgctgacggg
     4021 catcgagaac cgcaacctgc gctcgggcga ggccgccttc aaatacttcc tgctcggctc
     4081 gttcgcggcc gcgtttctgc tgctgggcat cgccttcgtc ttcggcgcca ccggtgagac
     4141 gcgctacgac gcgatccgtc aggttttggt cgacgggacg gcccagcccc gcctgctggc
     4201 catcggcttc tgcctgatgc tggtcggatt cggcttcaag ctgtcgctgg cgccgttcca
     4261 catgtacgcc cccgacgtct atgagggggc gccgacgacg accgcgtcgc tcatcgccac
     4321 ggggacgaag gtggccggat tcgcggcgct gttccatctg atcgagctgg ccgcggcgtg
     4381 gagcgacgcc gccggcgggc tgtggctggc gctctacgcg gtggcgctgg ccagcatgat
     4441 cgtcggcaat gtcggcgccg tcgtgcagcc caacatcaag cggatgctcg cctattcctc
     4501 aatcgcccac agcggctaca cgatcgtgcc gatggtcgtc atgctgcaac gccccgacct
     4561 gattgccgac gcgcgcgacg ccgtcgccta ttacatgctg gcctacacgg tgatgacgtt
     4621 gctggccttc ggcgtgccgg cggtcctggg cccggccggc gagtcgcgca tcgcgcaata
     4681 cgccgggctc catcgccgct cgccgatgct cgcctgcgtg atggcgctgg cgatggtctc
     4741 gctgatgggg atccccccca cgatcggctt cttcggcaag ttccagctct tcgccgtcgc
     4801 cgtccagggc gatcacatct ggctcgccgt cctcggcgtg ctggcctcgg tcgcctcggt
     4861 ctattactac ctgcgggtga tcgtcacgat gtatatggag gagccggcga cggccggcga
     4921 ggaactcgaa tcacgccggc tggacggagt taatgccttc gcgctgaccg cctgctcggt
     4981 ctgcgtcttc ctcttcgtcg tgttccagag tctctacctg ttcggagtct gatgccggca
     5041 agttcgcact catgagcgga gccaactgac cttatgtcct cgatctggat catcctgatt
     5101 cccctgctgc ccctgctggc gtttgtcgcc aacgggctct tcggccgcgc gctgcaacgg
     5161 accggcggct ggctggccgt cggctgcatg ggcggttcgg ccctcatcgg cgccgtcatc
     5221 ttcttcggcg gcctcttcgg cggcatcacg ttcccgatcc aggtcgagct gtggcagtgg
     5281 atgccgctgg gcgacgtcac gatccggatc ggtttctacg tcgatcagct caccgcgctg
     5341 atgctgttct tcgtcacgtt cgtctcgacg ctcgtctttc tctactcgat gggctacatg
     5401 gcgggcgacc ccggcttcaa ccgcttcttc gcctacctcg gcctgttcga tttcagcatg
     5461 ctggtgctcg tgctcggcga cagcctgccg gtgctgttcg tcggctggga gggggtcggc
     5521 ctctgctcgt atctgctgat cggctattac ctcgcgatgc cgggtgcgcc cgacgccggc
     5581 aagaaggcgt tcatcgtcaa ccgcatcggc gacttcggct tcctggtcgc gatgttcatg
     5641 ctgttcgcct ccgtcggcac gctcaacatt caggagattc tcgcggcggt tgcatccggc
     5701 gtcgtcccgg agttccaata cggcgccgcg ttcgtcacct tcctgaccct gatgctgttc
     5761 ctgggctgca cgggcaagag cgcgcagatc ccgctgttcg tctggctgcc cgatgcgatg
     5821 gccggcccca cgccggtcag cgcgctgatc cacgccgcca cgatggtcac cgcgggcgtc
     5881 tacctcgtgg tgcggctgtc ggcgctgttc gccctcgccc cggcgacgat gctcacaatc
     5941 gcaatcgtgg cggcggcgac ggcgttcgtc gccggctcga tcgccctcac gcagaaggac
     6001 atcaagaagg tcctggccta ttcgacggtc agccagctcg gctacatgtt cctcgcctgc
     6061 ggcgtcggcg cattcaccgc aggcatcttt cacgtcttca cgcacgcctt cttcaagggc
     6121 tgcctgttcc tcggcgcggg cagcgtcatc catgcctgcc atcacatcca ggatatggac
     6181 cagatgggcg gcctgcggcg caagatgccc gccaccgccc gcacgttcct gttcggctcg
     6241 ctcggtctgg cgggcattgt gccgttcgcc gggttcatgt cgaaggacga gatcctctgg
     6301 cgcgtcttca gcgacggcaa gttcatcctc tatgcgatcg cgctggtgac cgccgccttc
     6361 accgcctact acgccttccg cgcctacttc ctcacgttcg aggggcaaag ccggctgccg
     6421 cacgacgtcg agcatcatgt gcacgagtcg ccctggacga tgacgctgcc gctgtggatc
     6481 ctcggcgtcg gcgcgctcgg cgcgggcgtg ttcaacatcc tgcccgcctt cggccacggc
     6541 cactacagcg cgatcaacca cttcctggcg ccggtggtgg cgcccgccga ggcgatcctc
     6601 gcggcccacg cctcccacga accgatccac agcttcgcgc tggagatcct gctgatgatc
     6661 gtctcgacgg tcgtcgcgct cgtctgcggc gtcggcctgg cctggctgtt cgcctacaag
     6721 ctgtggcccg ccggcgcgag ccgcatggcc cgccgcctcg ggccgctcta ccgcatcagt
     6781 taccaccgct ggtggtggga cgagttttac aacgttttcg tcgccggcgg gctcaaactg
     6841 ctcgcgctca tctcgacgct gttcgaccgc ttcgtgatcg acggcgtgct gcatacggtc
     6901 gccggcgccg cccgctccgg cagccggctc atccggttct tccagaacgg ccaggtccag
     6961 gcctatgccc tcgccatcct cgtcggcgtc aatctcgtgg tctggctggt cctgtggtac
     7021 cagtgaattt aaggacacaa gggccataag gaaaggtttt cgagagccga ggacgagaga
     7081 cgaattcaac atgacgcgga gagcgctgac ccgacatgcc tgaactgacc aatcatcacg
     7141 ccgcactgct gctgctgatg acgtttctgc ccgccgtgac ggggatcgtc atcgccgcgg
     7201 ccgtgcccgc cgccctggcc cggaccgtcg ccctggccgg cgcggtcctg acgttcctcg
     7261 tttcgctcac gtttgtcggc gggatcttcg gcatgccggc cgactttacc gccgcggtca
     7321 acatcccctg gatcgagcgc tacaacgtcg ccctgctgat ggggctggac ggcctttccg
     7381 gttcgcttgt cctgctgacc aacgtcctga tcatcctcgc ggtgctcggt tcgtggaccg
     7441 ccatcgcggc gcgccagaag gagttttact tctacctcct cctgctgcag accggcatcc
     7501 tcggcgcctt cctcgccctc gacctgctgc tgttttacat cttctgggag atcatgctga
     7561 tcccgctcta ttttctgatc gggattttcg gctcgaagaa ccgcatctac gccacgacga
     7621 agttcttcct ctacacgatg gccggctcgc tgctgatgct cctcggcatc atcgcgctct
     7681 atttccacac cggttcgttc gcgctagccg acgccctggg cgccgccgcc acgctcgacc
     7741 cgaccgcgcg cctgctgatc ttcctctcgt tcgtgatcgc cttcgcgatc aaggtgccgc
     7801 tcttcccgct ccacacctgg ctgcccgacg cccacaccga ggcgccgacc gccggctcgg
     7861 tggtcctggc cggcgtgctg ctcaagacgg gcgtctacgg cctgctgcgc ttcgccatcc
     7921 cgctcttccc ggccgaggcc gcccgcattg ccccgctgat gatgctgctg gccgtcatcg
     7981 ccatcatcta cggcgcgctg accgccttcg cccagcgcga catgaagcgc ctggtcgcct
     8041 attcctcggt cagccacatg ggcttcatcg tcctgggggt cttcgccttc cgcgaggcgg
     8101 cgatcaccgg ggcgtcgctc cagatgatca accacggcat ttcgaccagc ggcctgttcc
     8161 tctgcgtcgg ctacctttac gagcgccgcc acacgcgcga actggcggcg ttcggcggcc
     8221 ttgcccacaa catgaagctc tacgccgtgc tgacgatgat catgattctt tcgtcggtcg
     8281 gtctgccggg gctcaacggc ttcgtcggcg agttcatgat tctcgtcggc gcgtatccgg
     8341 ccaacgccgt cctcacgatt atcgccgcca ccggtgtcat cctcgccgcc tgctacctgc
     8401 tgcgcctgat gcaggagacg ttttatggcc cgctggataa ggatgagaac aaggtcctgc
     8461 gcgatctgaa tccgcgcgag agcttcacgc tcgtcgtgct ggccgtcttc gccctctgga
     8521 tcgggctcta cccccggacc ttcaccgagc cgctggcgcc ggtctcggaa cggatcgtgc
     8581 gggcggtgga ggcggcccag agccccggaa ccgccctggc cgacgcaacc gcggggaaat
     8641 aacgcgtctt ctttatcaat tcggctctcg gctcgcgctt gattacgatt ccctctccaa
     8701 cgggcattcc gctccgagga agggcggttg ggccgctgaa cagcttcttc atgaacctca
     8761 agaaacggtc gaatcgaata cttttcgaga gccgaggacg agagccgaga gccgattaac
     8821 tgcaaggcgg tttgtgatgc ccaccgtgat tcttccccgc atcggcggct accaga
//
LOCUS       DAOPCO010000163         8868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7407_length_8868_cov_4.434585, whole genome shotgun sequence.
ACCESSION   DAOPCO010000163 DAOPCO010000000
VERSION     DAOPCO010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8868
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7407_length_8868_cov_4.434585"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLS90_11370"
     CDS             complement(<1..253)
                     /locus_tag="PLS90_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03046.1"
                     /translation="MTQNRYRVLVQCPPFPVADASYRPTFFDLDFDSYVEAGLIETKD
                     WSPDSIRVVRYDRETRQVIPASSGGDYLPCKVERWQRIKR"
     gene            complement(494..1108)
                     /locus_tag="PLS90_11375"
     CDS             complement(494..1108)
                     /locus_tag="PLS90_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03047.1"
                     /translation="MPRKHAFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVARVKA
                     DMRSIATALDSYIVDHNNYPWPVSQNQFIQYTTELTTPIAYMTSVMLQDPFLPDRFTL
                     QGWSIPADFIGTVQWVNYNGWWGNGYDGGGTLRDPKHRRAYCISSYGPDRSSNGIEWF
                     PLDEKYGAVMTDEDRYWRIYDATNGTISTGDIGRWGGEIAGVPQ"
     gene            complement(1540..7137)
                     /locus_tag="PLS90_11380"
     CDS             complement(1540..7137)
                     /locus_tag="PLS90_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03048.1"
                     /translation="MEPRGRFAQIKTGKSLLKASSMRIISCATALIWFVLCGSVAQAL
                     RVTVDPSQANGTTVFATLHAASEYISNHNDPGVDDRIDVMVDRLEENDGICGYDWGDR
                     LTIDGDGDRNGTWCTVVVGNNGTFKGNTYNCFFLMDISAAAPWQGRYVVKNFVLIPKF
                     IAANQNPPQVYSVFEPVMNFHQNTQGSVLFDNIVVTASLPGDEAANPFIDQRSMATRW
                     GRPFVLTARTQNVDARLTRIDIRNTLVSQTKGFGGVASVLYSHGDDQLIYLGPGFAAL
                     YCDGSRLIDVRRDARKSCLEIAGLPDNRNLFLGNSVTDELLNFEVEDYTLTRGWSRIA
                     FTDFIDNSATAVARTQAGLGLIDHCLFAENAAMSDPFVLASPSEKIAPEIKIKDCTFF
                     NMGAGSEIMHFGAGNFAGIGFRTNIATGPRTATFGQGLFAGNGADHDYVGIYPLHREL
                     AAPLFTDSDGALASLTKIDGNNCVVGEDTGAIIGGDGTFVMTFDPLDIKGVDSVSVTL
                     AAAAPRTNTYPNSGSYQSDLSVWLIVDGGPPFEIGRFRGDKANDFTTNMALDADMDGV
                     GEGTRLTSALRDFSFSSAISGDSLQVQIRMTSDQNQEEIVYDNVRIESSSFNVSQSFE
                     GYAGLSRYIFENVIYAAGSNETAASPATSDVAVNINAPVILRRCVFPESGPWRLRPPY
                     GETFPPAPLAEVGWANLNPMGATVFENCSNADPQFVSDDPSDYPLPDDLTSWQARKIT
                     LDGCDFLKPTQSQLPFNLAAQEIVKGALADDRTPVDTGLWRVRPLAQSYRNIWVSSDA
                     SQRFAEKPRLALCPTGRLVASMTYTTNSNQLGVVYTSDDKGQNWVHRRTFRFVGGRPF
                     VAGDNLYILGCDPDLVIMRSSDWGDTWEEPRKLSLNERWYGAAANVHYEGGQVYLVMS
                     RHLDRAQGTDPDRVADLAPVLMRAAVGDDLTLVENWTFAEAPSFVDLIDDKALDDFGV
                     PYYPAYQPETYTVAEGRLSWPSGWLDGNVTRILDSTHYWHGADEKTFHLLLRAQTGGA
                     GYGALLKVVEHADGSMATSLEEAPSGVTTTFLPLPGGQSGFCILYDEPTGLYWLLSTQ
                     TTDAMIWPEQLPSRRAGLPAAESRRLALHFSRNLVDWCFGGLVAFGPADQASRTDASM
                     VIDGDDLHVICSSGDLQAMDSDHCNRVTFHTVRDFRRLADYLVGTSGVNSVLPAVPDA
                     DDWRVRPLANAYTTLWHATAPRLKYRRDPALARCPNGRLIASGGEIGIYTSDDHGLSW
                     EFKADHNMSQARPFVAGQSLYLLGHKHDLTILRSDDWGETWDAFPSYLTSGQEWHQAP
                     CNVHYANGYVYIVMERQIPNQLPNGWWYVSELAPVLMRGKVNDDLRVPSNWTFASQVP
                     FYNEIDDRALQHLGIPFYPGYYPDRHPIVSGRIFYPAGWLETNVVQFVDPAHQWYDPA
                     GRTFHLFARTHSGRSNLAHVMKVIENDDGSMTTMAQTAPSGVKMHYVPMPGGHIKFHV
                     LYDEETRLYWLLSSQATDSMKRAEMLPEDRFDLPTNERERLQLHFSRNMMDWCFAGLV
                     AVGESSRAARHYASMVIDGDDLLILSRSGDETAKDAHNGNILSLHTIRNFRALAYLDD
                     PYGLSDEAYATHPGRDAVQDFESNSAGLGYTTNLDPAGSALSTHYGPSSALFIGNGVM
                     RDYYGVYPLHTQTDPPLFTDEDGALASITPLSGERFIAGEDTRARSLDGDRVTMRFEP
                     VDISSADEVAITLAAAAPRSPDAYANAPDSVSDLSLWIAVDEAPPLMIGRFRGDAQND
                     YTTNLSLDTDLDGVGDSVALSSAFQDFTFSAPVSGSALRAEVQLQSCQNDQEIAFDHV
                     RITPLVSIPASQTRCQVPFSLWE"
     gene            complement(7192..>8868)
                     /locus_tag="PLS90_11385"
     CDS             complement(7192..>8868)
                     /locus_tag="PLS90_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03049.1"
                     /translation="WSTFGTAITSGQELHQAPSNVLFSGNNVYLVMERRVPDELSGGW
                     YVSELAPVLMRANVNDDLTAASSWTYASEIPFYDEIDDAALNHLGIPFYDGFYPNSIT
                     LAPGRVFYPAGWLETHVVKFEDSSHEWYDPEGNTFHLFARTHTGRSNLACVMKVVENQ
                     DGTMTTSAQTAPSGVKTHYLPMPGGHIKFHVLYDEVSQLYWLVSSQATDSMTRAELLP
                     EDRVDLPTNERHRLQLHFSKNMVDWCFAGLVAVGEDNRSARHYCSMVIDGDDLLILSR
                     SGDECAESAHNVNMITLHKIESFRELAYLDDAYGIPDQTFTTTIQVSEAQSFESEMTG
                     LGFVNNISPNSNRVMATFETNFAETYFIGKGYWAPRDYIALWPLHTASPTNPTLFYDD
                     DDTLANLSNIHGDNCVVVEDSRTGEAGGDDDFLLMTFEPVDITGDDEISVTIAVAAPR
                     GTDAYANTAADTTDLSVWIIVDDGDPVEIGRFCGDVWNDSTTNLALDMDMDGVGEGTA
                     LTSTLQDFTFTYPVSGDELVVMVRAESRQNNQEIVFDNVRVKSVTVRPTP"
BASE COUNT         1859 a         2680 c         2538 g         1791 t
ORIGIN      
        1 cacgcttgat gcgctgccac cgctcgacct tgcacggcag atagtcgccg ccgcttgaag
       61 ccggaatgac ctgccgggtt tcccgatcat agcgcacgac ccgaatcgag tcgggactcc
      121 agtccttcgt ttcgatcagt cccgcctcaa cataggagtc aaaatccagg tcgaaaaatg
      181 tgggccgata ggatgcgtcc gcgacaggaa agggcggaca ctgcaccaga acccggtatc
      241 ggttctgagt cacccaggca tacccatgag gatcggcgat cgattggacc cgcgggggcg
      301 ccaccgagtc tgggtcggcc tcctggcacg gtccatctgc cggcaaagcc agccagatcg
      361 cggcgaacaa gaacagcagg gaactgcaac catgccttct acgcatctcg gacctcggat
      421 atcattggat gggtgacctg gccttgcgat catccagcca ggtcacggca tcacccttcg
      481 caaagaggcc tgctcattgg ggaaccccgg cgatttcgcc accccagcgg ccgatatcgc
      541 ccgtgctgat ggttccattg gtcgcgtcat aaatgcgcca gtatctgtcc tcgtcggtca
      601 tcaccgcacc gtatttctca tccagcggga accactcgat cccattggag ctgcggtcag
      661 gcccatagct ggagatgcaa taagccctgc ggtgcttggg gtcacgcagt gtgccgccgc
      721 cgtcatagcc gttgccccac cagccgttgt agttgaccca ctgaaccgtc ccaatgaagt
      781 cggccggaat gctccagccc tgaagcgtga accggtcggg caggaagggg tcctgcaaca
      841 tcacgctggt catgtaggca atcggggtgg tcagttcggt ggtgtactga atgaactggt
      901 tctgactgac aggccaggga tagttgttgt ggtcgacaat gtagctatcc agcgcagtcg
      961 cgatcgaccg catgtcggcc ttgactcggg cgaccttcga gcgggtttgt gcctccagga
     1021 agttcggcac cgcgatggcc gccaggatcg caatgatcgc caccacgatc agaagctcga
     1081 tcaacgtaaa cgcatgcttt cttggcatga ttcccctcct tgctcccgca aaatggtttc
     1141 aaaaggtgtt tttgccaaac cagccgtctt ttcgttcctc tttccgaggc attgaaggta
     1201 ttgaaaagcg ccggcgttgc ggcttcccgg ggagaccttc ggctgacaag cgactctcct
     1261 gcgccccgcc caaaatggcc accgttcatc atcccttgcg catactaggc gaggaacgac
     1321 aaccttgtca accttattct gcaagctatt gtgccgccgc taacgcccaa atatccctcc
     1381 tttttatggg ttttttgccg tctggtggat acccgtgcac gctctctggg accctctgag
     1441 attggattta caccgtttgt catttctggc catttcctaa gggcttctac ccctgccctc
     1501 cgggctctgg cgacggaaag atcgccgtca ttcagtgact cactcccaaa gggaaaaggg
     1561 gacttggcaa cgagtctgcg aggccgggat gctcacaagt ggggtgattc gcacgtggtc
     1621 gaaggcaatt tcctggtcgt tctggcagct ttgcagctga acctccgccc tcagggccga
     1681 tcccgaaacg ggagcgctga aagtgaaatc ctgaaaggcc gagctcaagg ccacggagtc
     1741 ccccaccccg tccaggtccg tgtccaggct caggttggtg gtgtagtcat tctgcgcatc
     1801 gccgcgaaac cggccgatca tcagcggcgg ggcttcatcg accgcaatcc acaggctcag
     1861 gtcgctgaca ctgtccgggg cgttggcata ggcatcgggg gatcgcggag ccgccgcggc
     1921 cagggtgatc gctacctcat cggcactgga gatatcgacc ggttcaaagc gcatcgtcac
     1981 ccggtcgccg tccagcgacc tcgcccgcgt atcctccccc gcgataaagc gctcgccact
     2041 gagcggggta atcgacgcca gagcgccgtc ctcgtccgtg aacaacgggg gatcggtctg
     2101 cgtgtgcagg ggataaaccc cgtaatagtc tcgcatcacg ccgttgccga taaagagcgc
     2161 gctcgagggg ccataatgcg tgctcagggc gctccccgcc gggtcgagat tggttgtgta
     2221 gcccaatccc gccgaattgc tttcaaagtc ctgcaccgcg tcccgaccgg ggtgcgtcgc
     2281 ataggcctcg tcgctcagac cgtaagggtc gtcgaggtag gccagcgccc ggaagttccg
     2341 gattgtgtgt aatgacagga tgtttccgtt atgcgcatcc ttggccgttt catcacccga
     2401 gcggctgaga atcaacaaat cgtccccgtc gatcaccatg ctggcatagt ggcgcgccgc
     2461 ccggctcgat tctccgacgg cgaccagccc ggcgaaacac caatccatca tgtttcgtga
     2521 gaaatgcaac tgaagccgtt cgcgttcgtt ggtcggcaag tcgaagcgat cctcgggcag
     2581 catttcggca cgcttcatcg agtcggtcgc ctgggaactg agcagccagt aaagccgcgt
     2641 ctcttcatca tacagcacgt ggaatttgat atggccgccc ggcatgggca cataatgcat
     2701 tttcacgccg gaaggagccg tctgggccat ggtcgtcatc gaaccatcgt cgttctcgat
     2761 caccttcatt acgtgcgcta aattggagcg tcccgagtgg gtgcgcgcaa acaagtggaa
     2821 cgtccgaccc gcggggtcat accactggtg cgccggatcg acgaattgga cgacgttggt
     2881 ctccagccag cccgccggat agaaaatccg gccgctgacg atcggatgac ggtcgggata
     2941 atacccgggg taaaacggaa tccccaggtg ctggagcgcg cgatcgtcga tttcattgta
     3001 gaacggcacc tgcgaagcaa acgtccagtt ggacggcact cgcaaatcgt cgttcacctt
     3061 gccgcgcatc agcaccggcg ccagttccga gacataccac cagccgttgg gcaactgatt
     3121 cgggatctgg cgttccatca cgatgtagac gtagccattg gcataatgga cgttgcaagg
     3181 cgcctggtgc cactcctgcc cactggtcag ataactcggg aaggcgtccc aggtttcgcc
     3241 ccagtcgtcg gagcgcaaaa tggtcaggtc gtgcttgtgc cccaacagat acaggctctg
     3301 gcccgccacg aagggacgcg cctgcgacat gttatgatcg gctttgaact cccaactgag
     3361 cccgtgatcg tccgacgtat agatgccaat ctcgccgccc gatgcgatca ggcgaccgtt
     3421 ggggcaccgg gccagggccg ggtcgcggcg gtacttcagg cgcggcgccg tcgcgtgcca
     3481 cagcgtcgtg taggcattgg ccagggggcg cacgcgccaa tcgtccgcgt cgggcacagc
     3541 cggcagaacg gagttgacgc ccgacgtgcc cacgagatag tccgccaatc gccggaagtc
     3601 ccgcactgta tggaaagtca cccgattgca gtggtccgag tccatagcct ggaggtcgcc
     3661 cgaagagcaa atcacatgga gatcgtcccc gtcgatgacc atgctcgcgt cggtgcgcga
     3721 cgcctgatcg gcgggtccaa aggctacgag ccctccgaaa caccaatcga ccagatttct
     3781 ggaaaaatgg agcgcgagcc gtcgcgattc ggcggctggc aggcccgcgc gacgactcgg
     3841 caactgctcg ggccaaatca tcgcatcagt cgtttgcgtc gaaagcagcc agtacagccc
     3901 cgtcggttcg tcatacaaaa tgcagaatcc cgactgtccg ccgggcaacg gcaagaaggt
     3961 cgtcgtgact ccggacgggg cttcttccag gcttgtggcc atgctgccgt cggcatgctc
     4021 gaccaccttg agcagcgcgc cgtaacccgc accgccggtt tgggcgcgca acaacaggtg
     4081 gaatgttttt tcgtcggcgc cgtgccagta gtgagtgctg tccaggatgc gcgtcacgtt
     4141 gccatcgagc cagccgctgg gccagctcaa gcgaccctcc gcgaccgtat acgtttcggg
     4201 ctggtaggcc gggtagtagg gcacgccaaa atcatcgagc gccttgtcgt cgatgagatc
     4261 gacgaagctt ggcgcctcgg cgaacgtcca gttttcgaca agcgtcaggt cgtcgcctac
     4321 ggccgcccgc atcaaaaccg gcgccaggtc ggccacccgg tcgggatccg tcccttgcgc
     4381 ccgatccaga tgcctgctca tcacaagata gacctggccc ccctcatagt gaacattggc
     4441 cgccgcccca taccaccgct cgttaagact cagcttgcgc ggctcttccc aggtatcgcc
     4501 ccaatcgctg gaccgcatga tcacgaggtc cggatcgcag cccaggatgt atagattatc
     4561 cccggcaacg aaaggacgtc ctcccacgaa gcggaacgtc cggcggtgca cccaattctg
     4621 ccccttgtcg tcggaagtat agacgacgcc cagttggttg ctgttggtcg tataggtcat
     4681 cgatgcgacc aaccgcccgg tgggacagag cgccagccga ggcttttcgg caaatcgctg
     4741 cgatgcatcc gaagataccc agatattgcg gtaagattgc gccaacggcc gcacccgcca
     4801 caacccggta tccaccgggg tccggtcgtc ggccaaggcc cccttgacga tctcctgggc
     4861 ggccagattg aaaggcaact gcgactgcgt cggcttgagg aagtcgcaac catccagggt
     4921 gatcttgcgc gcctgccagg aagtcagatc gtccggcaat ggataatcgg acgggtcatc
     4981 cgagacaaat tgcggatccg cattggagca attctcaaaa accgtggcgc ccatgggatt
     5041 cagattggcc caaccgacct ccgctaacgg cgcgggggga aaggtttcgc catagggcgg
     5101 tcgcagccgc caggggccac tttccgggaa gacgcagcgg cgtaaaatga ccggggcgtt
     5161 gatattcacg gcaacgtccg aagtcgcggg agaagccgcg gtctcattgg atcccgcggc
     5221 ataaatgacg ttttcgaaaa tataccgaga caaccctgcg taaccttcga agctttgaga
     5281 cacgttgaat gacgatgact caattcgcac gttgtcatag acaatctctt cttgattctg
     5341 atcgctcgtc atgcggatct gcacctgaag cgagtcgccg gagatggccg atgaaaaact
     5401 gaaatcacgc aaggcgctgg tcaggcgagt gccttcgcca accccgtcca tgtccgcgtc
     5461 gagcgccata ttcgtcgtga agtcattggc tttgtcgcca cggaagcgcc cgatttcaaa
     5521 cggagggccc ccatccacga tcagccagac actcagatcc gactgatacg atccggaatt
     5581 cggataggta ttggttcggg gggcggcggc ggccaacgtg acgctcacgc tgtcgacacc
     5641 cttgatatcc aggggatcga acgtcatcac aaacgtcccg tcgccgccga taattgcacc
     5701 ggtgtcttcc ccgactacgc aattgttccc atcgatcttc gtcagcgaag ccagtgcacc
     5761 atcgctatcg gtgaacaagg gcgcggcaag ctcgcgatgc agggggtaaa tgccgacgta
     5821 atcgtgatcg gcgccgttac ccgcaaaaag cccctgcccg aaggtcgccg tccgcgggcc
     5881 ggtggcaatg ttggtcctaa agccgatccc cgcaaagttc ccggctccga aatgcataat
     5941 ctcgctgcct gcccccatat tgaaaaaggt gcagtctttg atttttatct caggcgcgat
     6001 cttctcggaa ggggacgcca atacgaatgg gtcgctcatc gcggcatttt cggcgaacag
     6061 gcaatgatcg atcagcccca aaccggcctg agtgcgcgcc acggcagtgg cggaattgtc
     6121 gataaaatcg gtaaaggcga ttcgcgacca gcctcgggtc aaggtgtaat cttcgacttc
     6181 gaaattgagc agctcatcgg tgacggagtt ccccaggaac agattgcgat tgtccggaag
     6241 acctgcgatc tccaggcagc tcttgcgtgc atcgcgccta acgtcaatca gccgcgagcc
     6301 gtcgcaatac aatgccgcga accccgggcc cagataaatc aactggtcgt cgccatgcga
     6361 gtagagcacc gatgcaacac caccgaatcc tttcgtttgc gagactagcg tattacggat
     6421 atcgattcgc gtcagccggg cgtcgacgtt ttgcgtgcgg gcggtcaaga caaacggccg
     6481 gccccagcgc gtcgccatcg atcgctgatc gataaaagga tttgcggcct catcccccgg
     6541 gagactggcg gttacgacaa tattgtcgaa cagcaccgag ccttgcgtgt tctggtgaaa
     6601 attcatcacc ggctcaaaaa cactgtagac ctgcggggga ttttgattcg cggcaatgaa
     6661 tttcggaatc agaacaaagt ttttcaccac gtagcggccc tgccagggag ccgcggccga
     6721 aatgtccatc aggaagaagc agttgtaagt gttccccttg aaagtgccgt tattgccgac
     6781 aaccaccgta caccaggtgc cgtttcggtc cccatcgccg tcgatcgtca gccggtctcc
     6841 ccaatcatag ccacaaatac cgtcgttttc ctccaatcga tcgaccatca cgtcgatgcg
     6901 atcgtcgact cccggatcgt tatgattcga gatatattcg cttgcggcat ggagcgtggc
     6961 aaaaacggtc gtgccgtttg cctgggaggg atcgaccgta acccttaacg cctgcgccac
     7021 ggagccacac agcacaaacc agatcaaagc ggtggcacaa ctgatgattc tcatggatga
     7081 tgctttcaaa agactttttc cggtcttgat ctgagcgaac cggccgcgtg gttccatcta
     7141 taaaggttcc gccgcgcact ccataggttc aggaacaatg gtctcaagca atcaaggcgt
     7201 cggcctcacc gtcactgatt tcacccggac gttgtcaaaa acgatttcct ggttgttctg
     7261 tcgactctcg gcgcgcacca tgacgaccag ttcatcaccc gagactgggt aggtgaaggt
     7321 gaaatcctgc agcgtactgg tcaacgccgt tccttcgccg acgccatcca tgtccatgtc
     7381 cagggccaga tttgtcgtgc tatcgttcca gacgtcgccg cagaagcgcc cgatctcaac
     7441 tgggtcgccg tcatcgacga tgatccaaac gctcaggtcc gtcgtgtcgg ccgcagtatt
     7501 cgcataggca tcggtacctc gcggggccgc cacggcgatc gtcacggaga tttcgtcatc
     7561 tccggtaatg tccacgggtt caaaggtcat gagcaggaag tcgtcgtccc caccggcctc
     7621 gcctgttcgt gagtcttcca ccaccacgca attgtcgcca tgaatgttgc tcaagttcgc
     7681 gagcgtgtca tcgtcgtcat agaagagcgt cggattggtt ggggaagcgg tgtgcaatgg
     7741 ccagagagcg atgtaatcgc gcggagccca gtaccccttg ccgatgaaat acgtttcggc
     7801 gaagttggtt tcaaaggtgg ccatgacacg attggagtta ggggagatgt tgttgacgaa
     7861 acccaggcca gtcatctcgg attcaaagct ctgcgcttcg cttacctgga tggttgtcgt
     7921 gaacgtctga tccggaatcc cataggcgtc atcaagatac gcgagttcgc ggaaactctc
     7981 gattttgtgc agcgtgatca tgttgacatt gtgagccgac tcggcgcact cgtctcccga
     8041 tcggctcagg atcaacaaat catccccatc gatcaccata ctgcagtaat gccgtgcaga
     8101 gcgattatcc tcaccgacgg ccaccagacc ggcgaaacac caatcgacca tattcttcga
     8161 gaagtgcagt tggaggcggt ggcgctcatt ggtcggcaag tcgacccgat cctcaggcag
     8221 tagttccgcc ctggtcatcg agtcggtcgc ttgagaactg accaaccagt aaagttgaga
     8281 gacctcgtca tacagcacgt ggaacttaat atggccgccc ggcatcggca ggtaatgcgt
     8341 cttgacgccc gagggagcgg tctgggccga tgtggtcatc gtcccatcct ggttttcaac
     8401 caccttcatt acgcaggcga ggttgctgcg ccccgtgtgc gttcgcgcga acaggtggaa
     8461 tgtgttgcct tccgggtcat accactcgtg agacgagtct tcgaacttca ccacgtgtgt
     8521 ttcgagccac cccgccggat agaacacacg gcctggcgcc agagtgatgc tattgggata
     8581 gaaaccgtcg tagaagggga tgcccaaatg attcagcgcg gcgtcgtcga tttcatcgta
     8641 aaaggggatt tccgaagcat aagtccaact gctggccgcc gtcaggtcat cattgacatt
     8701 ggcgcgcatc aggaccggcg ccaattccga aacataccac ccgccgctga gttcgtccgg
     8761 cactcggcgt tccatcacaa gatagacatt attgccggaa aaaaggacgt tcgaaggggc
     8821 ttgatgcaat tcctgtccac tggtgatcgc cgtgccaaag gtactcca
//
LOCUS       DAOPCO010000164         8808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7466_length_8808_cov_4.942077, whole genome shotgun sequence.
ACCESSION   DAOPCO010000164 DAOPCO010000000
VERSION     DAOPCO010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7466_length_8808_cov_4.942077"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /gene="fliD"
                     /locus_tag="PLS90_11390"
     CDS             <1..593
                     /gene="fliD"
                     /locus_tag="PLS90_11390"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPK03050.1"
                     /translation="EIVAKLNEEFSNGGLGAQAINDGGKLKITSTAYGSEETITVVSN
                     QDAGAVNQLGIGTTKLSASGVDVAGTIAGITATGSGQVLRGAENSHVAGLELEVTTKT
                     PLSTEVHYSKGISTSVFEQLDNLTKASTGLFDTRERSYTRQITDLDDRVDVLNQRLDT
                     EEAAMRKKFTALETKISALQTQGNFLLSQLSSLIAT"
     gene            697..1125
                     /gene="fliS"
                     /locus_tag="PLS90_11395"
     CDS             697..1125
                     /gene="fliS"
                     /locus_tag="PLS90_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPK03051.1"
                     /translation="MRPQQNYRRVQIATADPTQIVVMLYEGAISNLNQTQRLLGTDDA
                     KALERVARTQEIINYLRSVLDHQRGGEISANLERLYEYMRDKLNETLIQRTPAPVGEV
                     IHLLGILLEAWRGVAQQSAEPEPADEPAAAPPQRSFSFMG"
     gene            complement(1160..1807)
                     /locus_tag="PLS90_11400"
     CDS             complement(1160..1807)
                     /locus_tag="PLS90_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HPK03052.1"
                     /translation="MPSRRRFPIDPEAYFPAIVAAIGLVIGYAFGWWILWVPCLIALV
                     LLLGFFRDPPRRVPQIPGAVVSPADGKVTAITVNQDPEAGPAGGPVISIFLSVLDVHI
                     NRAPYAGRITGLRYRPGDFLNALNVDSGKRNEANWIFMECGRHHLTVRQIAGLIARRI
                     VCRVRKGDRVRRGQRIGLIRFGSRTELYLPPEARVQVEVGQRVSGGRTVVAYLPE"
     gene            complement(1982..3205)
                     /gene="dinB"
                     /locus_tag="PLS90_11405"
     CDS             complement(1982..3205)
                     /gene="dinB"
                     /locus_tag="PLS90_11405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPK03053.1"
                     /translation="MAETLPASDPRSLRGRRDILHVDMDAFFAAIEVLDQPALAGKPI
                     IVGGTPEQRGVVATASYEARRYGIHSAMSSWKARQLCPHAIFLPPRRRRYVECSRQVF
                     AIFGDYTPLVEQVSIDEAFLDVTGNRRLFGAPETIAREIKRRIRQEVGLTASVGIAPN
                     KFLAKLASDMEKPDGLVVVAPERIDAFLLGLPVGKLWGVGEKTRRRLSELGIRTVADL
                     RNYPVELLEQHFGTQGLAMRELAFGRDDRPVVAASEAKSIGSETTFARDIAEPARLRA
                     ILDELAEKVARRLRREGLRARTVHLKARYADFTTVTRSETIETATARSGAIRDLARRL
                     FDQRLGRPSLPMRLLGVSVSNLEPAGAGQIDLFPDPREQKDEALDHLLDRIGEKYEKT
                     AIQRGLTHAAADDDD"
     gene            complement(3261..5411)
                     /locus_tag="PLS90_11410"
     CDS             complement(3261..5411)
                     /locus_tag="PLS90_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK03054.1"
                     /translation="MIAPHLSWPAALLWLILGVAVAPAVRAESTPADKPPRHAGRTEI
                     SEEPWTPPPPVIRPQVIYGSDDRIDVYEEQDPLRQRLARATCGLFSSSRVVNNGDGTY
                     TLSLSSWRYSNLPPCSGEPFAGQPVGAFCTGFLVGPDLIATAGHCYDASDLSATRFVF
                     GFQMLDGQTPVRKVDASQVYRGIQIVGRQETSTIDYAVIRLDRPVTAPGALPLPIRRS
                     GTVPIGTRVGAIGHPAGLPLKLAFGAQTEVKRNSHTGYFEANLDTYGGNSGSPVFNAD
                     TGVVEGILVRGAPDYVVRSNCFESNRLSDSEGTEDVSKTTTFVQFIPPLSPSLRFLAD
                     VYGCQQTLEIELYRPEGADAAQVIVTSLRGDAEELTLTPAGDPRYLIGTLLLVPIDNT
                     IEPGNGRLETWHLDEVSVATLDGELSDTALIDCMPFDRVAVQAIETGIHHLTLDFRTS
                     DSASIVAEIFAEGSSERKTLLGETKVKQRQITVSGLEPCTLYTVEIISLTDLAGNLYQ
                     PPPRDRVFHFRTKVPGPHTLFEPFDSGAAAGWTHASTAGPDRWMARQHAFAVSQPYVF
                     SYLPAAAEVTDARLVSPVVEHATEVMTFWHTYEFEEGYDGGVIEISTNGGASWTDLGP
                     YIVEGGYDGVISTSYNSPIGGRGAWTGGALGAMRHVRIDLSSFPPPFQIRLRFTTDVS
                     VVPDGGGGWLIDDVQINEAVVCSQSGSSGWTSYH"
     gene            complement(5543..7060)
                     /gene="xylB"
                     /locus_tag="PLS90_11415"
     CDS             complement(5543..7060)
                     /gene="xylB"
                     /locus_tag="PLS90_11415"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HPK03055.1"
                     /translation="MLREILIGVDLGTSSIKVVAFDRAGRALASAARPCALSQPRPNW
                     SEQDPSAWWRAATQAMKAVSRRIDPRRVAGISFSGQMHGSVFLDARGEVIRPCLLWND
                     GRTAPQCEEIIRTVGREPLLRVLRNLPLTSFTLPKLLWLKQNEKANYRRLRTLLLPKD
                     YLRYRLTGRAAMDESDAAGTVMKVIGERRWAGEILAQLGLDAAVLPPLIGSAEIAGEL
                     TPAAARATGLVAGTPVIAGGGDQPVGAVGVGVFVEGQAMVSLGTSGVVLAPTRAVHLG
                     GEHGLASFDHAVAGSRYLMGCVISAAGSLQWFGDALCQAEAAEAKKARRNLFDLLLER
                     AAQTPAGAEDLWFLPYLMGERTPHPDPNARGVFFGLSTRHQKAHLTRALIEGVNFALR
                     DSLELMRARGLAINDIRVTGGGARNPFWLQNLADILGQPVRPVENPEGGAMGAALLAG
                     VGAGLYKDFNEAARRAIRVGDRVRPRRRDVRDYEQRYQRYRRLYPALKDLFVQYS"
     gene            complement(7195..>7644)
                     /locus_tag="PLS90_11420"
     CDS             complement(7195..>7644)
                     /locus_tag="PLS90_11420"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HPK03056.1"
                     /translation="QDIIWVGGGNTANMLAVWRVHGVDRILREAWEAGVILTGGSAGS
                     LCWFECGTTDSFNLFQLAPLHDGLGFLPGSHCPHYDGEAQRRPLYQRLIAEGFPAGYA
                     LDDDAALHFEGTRIMEAVAARPEAGAYRVERVGGQVTEARLPIRLLA"
     assembly_gap    7645..7654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7655..7867)
                     /locus_tag="PLS90_11425"
     CDS             complement(<7655..7867)
                     /locus_tag="PLS90_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HPK03057.1"
                     /translation="MAKPRRIIPFGGLRPSPGRTHPLVDYLLDLTGKQRPRVAFIPTA
                     MGDAADALLGMYNRIPASRAERTHLAL"
     gene            complement(7880..8677)
                     /locus_tag="PLS90_11430"
     CDS             complement(7880..8677)
                     /locus_tag="PLS90_11430"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPK03058.1"
                     /translation="MILTVDIGNTHIHMGLFIRGALRQTWSIASYPPRTADEYALLMS
                     GMIGEDMPMFGAIISSVVPRLEQPVIRAIEKGFKTTPMVLDRDTPIGITNGYEHPSEV
                     GMDRLANAVGANYFYGPPVMVLDFGTAITLDYLAPAPTAEHLPIYKGGAILPGIEMAV
                     EALARGTARLPQVPLHEPRRVIGRTTAESIQAGIMHGYLGAITTLVDRSCEEIGEACP
                     VIATGGDALNLRQHLPFLRAVVPELTHWGLRHIYGMNNDCPLPAPPR"
BASE COUNT         1449 a         2861 c         3109 g         1379 t
ORIGIN      
        1 ccgagatcgt cgcgaaattg aacgaggaat tctccaacgg cggcctcggc gcgcaggcga
       61 tcaacgacgg cggcaagctg aagatcacct cgaccgccta cggcagcgag gagacgatca
      121 cggtcgtgtc gaatcaggac gcgggggcgg tcaaccagct cggcatcggc acgaccaagc
      181 tgagcgcgtc gggcgtggat gtggctggaa cgatcgccgg gatcaccgcg accggatcgg
      241 gccaggtgct taggggggcg gagaattcgc acgtcgccgg gttggaactg gaggtcacca
      301 ccaagacccc gctgtcgacc gaagtgcact acagcaaggg gatctcgacg agcgtgttcg
      361 agcagttgga caacctgacc aaagcgtcga ccggactgtt tgacacgcgc gagcggagct
      421 acacgcgcca gatcaccgac ctggacgacc gggtcgacgt gctgaaccag cggctggata
      481 ccgaggaggc cgcgatgcgc aagaaattta ccgcgctgga gacgaaaatc tcggcgctgc
      541 aaacccaggg caacttcctg ctgagccagc tgagttcact gatcgcaacc tgaaccgatt
      601 aagttaaaga ttacctgccc agcggccgat taggaccatg gcgtcctgac tcgcgaggcc
      661 gccccgccat cgcaatcccg ggagcatgag gagctgatgc gtccacaaca aaactaccgt
      721 cgcgttcaga tcgcgaccgc cgacccgacg cagatcgtcg tgatgctcta cgagggcgcc
      781 atcagcaatc tgaaccagac gcagcgcctg ctggggaccg acgacgccaa ggcgctcgaa
      841 cgcgtggcaa ggacccagga gatcatcaat tatctgcgca gcgtgctgga ccatcagcgg
      901 ggtggcgaga tttcggctaa tctggagcgg ctctacgaat acatgcgcga caagctcaac
      961 gaaacgctga tccagcggac gccggcgccg gtgggcgagg tgatccacct gctcgggatt
     1021 ttgctggagg cctggcgcgg cgtggcgcag cagagcgccg aaccggaacc ggccgacgaa
     1081 ccggccgcgg cgccgccgca acgttcgttt tccttcatgg gctgaaaatc ggagcggttc
     1141 tgaagttcac cgaccggcgt tattcgggca ggtaggcgac cactgtccgg ccgccgctga
     1201 cgcgctggcc gacctcgacc tggactcgcg cctcgggcgg caggtagagc tcggtgcgcg
     1261 acccgaagcg gatcaggccg atccgctggc cgcggcgaac gcgatcgccc ttgcgcacgc
     1321 ggcagacgat ccgccgggcg atcagccccg cgatctggcg cacggtcagg tgatggcgcc
     1381 cgcactccat gaaaatccag ttggcttcat tccgcttccc gctgtcgacg ttgagcgcgt
     1441 tgaggaaatc gcccggccgg tagcgcaggc ccgtgatgcg gccggcatac ggcgcgcggt
     1501 tgatgtgcac gtcgagcacc gagaggaaga ttgagatcac cggcccgccc gccggaccgg
     1561 cttcggggtc ctggttgacc gtgatcgcgg tgaccttgcc gtcggccgga ctgaccaccg
     1621 cccccggaat ctgggggacg cgccgcggcg ggtcgcggaa gaatcccagc agcagcacca
     1681 gcgcgatcag gcacgggacc cacaagatcc accagccgaa cgcatagccg atgacgaggc
     1741 cgatggcggc gacgatcgcg gggaagtaag cttcggggtc gatcggaaag cgacggcggc
     1801 tgggcatggt ggaacgtgcg tttccttggg gcgcttctcg gcctattatg aggccgttgc
     1861 caggcgatgc caatcatttt cccgggggcc aaagagcgcg ccgattgaaa atcggtcgga
     1921 tccgacggat ccgtccgatc cgacggcttg cgatccgctg cttctgagcg ggcgggatga
     1981 atcaatcgtc gtcgtcggcg gccgcgtgcg tcaggccccg ctggatcgcg gttttctcgt
     2041 atttctcgcc gatccgatcg agcagatggt ctagcgcctc atccttctgc tcgcgggggt
     2101 cgggaaacag atcgatctgc cctgcgcccg cgggctcgag gttcgaaaca ctgacgccga
     2161 gcaggcgcat cggcagggac ggccggccca gacgctggtc gaacagccgc cgcgccagat
     2221 cgcggatcgc accgctccgg gcggtcgcgg tctcgatcgt ttcggagcgc gtgacggtgg
     2281 tgaaatcggc gtagcgcgcc ttgaggtgga cggtgcgcgc gcgcaggccc tcgcggcgca
     2341 ggcggcgcgc caccttttcc gccagctcgt cgaggatcgc gcgcagccgc gccggctcgg
     2401 cgatgtcgcg ggcgaacgtc gtttccgagc cgatcgattt ggcttccgag gcggcgacta
     2461 ccggccggtc gtcgcggccg aacgccagct cccgcatcgc caggccctgc gtcccgaagt
     2521 gttgttccag cagttcgacc ggatagttgc gcaggtcggc gacggtgcgg atgcccaact
     2581 cgctcagccg ccggcgcgtt ttttcgccga cgccccacag cttgcccacc ggcaggccga
     2641 gcaggaaggc gtcgatgcgt tcgggcgcga cgacgactag gccgtcgggc ttttccatgt
     2701 cgctggccag cttggcgagg aatttgttcg gcgcgatccc gaccgaggcg gtcaggccga
     2761 cctcctggcg gatgcggcgc ttgatctcgc gggcgatggt ttccggcgcg ccgaacagcc
     2821 gccggttgcc cgtcacgtcg agaaacgctt cgtcgatgga gacctgctcc accagcggcg
     2881 tgtagtcgcc gaaaatggcg aagacctggc gcgagcattc gacgtagcgc cgccgccgcg
     2941 gggggaggaa gatcgcgtgc gggcagagct ggcgcgcttt ccaggagctc atcgcgctgt
     3001 gaatgccgta acgccgcgct tcgtaggagg ccgtcgcaac gacgccgcgc tgctcgggcg
     3061 tgccgccgac gatgatcggc ttgccggcca gcgcgggttg atcgagcacc tcgatcgcgg
     3121 cgaagaacgc gtccatgtcg acgtgcagga tgtcgcgccg gccgcgcaac gaacgcggat
     3181 cggaagctgg aagagtctcg gccatcgcgt gccccggagc cgcggccggg ccgccggctc
     3241 cgccggccat catacggcgc tcaatggtag gacgtccagc cgctgctccc ggattgggaa
     3301 cacacgacgg cttcattgat ctggacgtcg tcgatcagcc atccgccccc gccgtcgggg
     3361 acgacgctga cgtcggtcgt gaagcgcagg cggatctgga atggcggcgg gaacgagctc
     3421 aggtcgatgc ggacgtggcg catcgccccc agggcgccgc cggtccacgc gccgcggccg
     3481 ccgatcgggc tgttgtagct cgtgctgatc acgccatcgt agcccccctc gacgatatac
     3541 ggtcccaggt cggtccagct ggcgccgccg ttggtcgaga tttcgatcac gccgccgtcg
     3601 taaccctcct cgaactcata ggtgtgccag aaagtcatga cctcggtggc gtgctccacg
     3661 acgggcgaaa ccagccgcgc gtcggtgact tcggccgccg ccggcagata gctgaagaca
     3721 tagggctggc tgacggcgaa agcgtgctgg cgggccatcc agcgatcggg acccgcggtg
     3781 gaggcgtgag tccagccggc ggcggcgccg ctgtcgaagg gctcgaagag cgtgtggggc
     3841 cccggcacct tcgtccggaa atggaacacc cggtcgcgcg ggggaggctg atagaggttg
     3901 ccggccaggt cggtcaggct gatgatctcg accgtgtaga gcgtgcaggg ctccaggccg
     3961 ctgacggtga tctggcgctg cttgaccttg gtctcgccca gcagggtttt gcgttcgctg
     4021 gagccctcgg cgaagatctc cgcgacgatg ctcgccgagt cgctcgtgcg gaaatcgagc
     4081 gtcagatgat gaattcccgt ttcgatggct tgcacggcga cgcggtcgaa cggcatgcag
     4141 tcgatcaggg cggtgtcgct gagctcgccg tccagcgtcg cgacgctcac ttcatccaga
     4201 tgccacgtct cgagccggcc gttgcccggt tcgatcgtgt tgtcgatcgg cacgagcagc
     4261 agcgtgccga tgaggtaacg cggatcgccg gcgggggtca gcgtcagctc ctcggcgtcg
     4321 ccgcgcaggg acgtcacgat cacctgcgcc gcgtcggcgc cttcgggtcg atacaattcg
     4381 atctcaagcg tctgctggca gccgtagacg tccgccagga accgcagcga aggactgagc
     4441 ggcgggatga actggacgaa cgtggtggtc ttgctgacgt cctcggtgcc ctcgctgtcg
     4501 ctcagccggt tggattcgaa gcagttggag cggacgacgt aatcgggtgc gccgcgcacg
     4561 aggatgcctt cgacgacgcc ggtgtcggcg ttaaagaccg gcgagccgga gttgccgccg
     4621 taggtatcca ggttcgcctc gaagtagccg gtgtgggaat tgcgtttgac ctcggtctgt
     4681 gctccaaagg ccagcttgag tggcagcccg gcggggtgcc cgatcgcgcc gacgcgcgtc
     4741 ccaatgggga ccgtcccgct gcgccggatc ggcaaaggca aggcgcccgg ggcggttacc
     4801 ggccggtcca gccggatgac cgcgtagtcg atcgtgctgg tctcctggcg accgacgatc
     4861 tgaattccgc ggtagacctg gctggcgtcg accttgcgca cgggggtctg gccgtcgagc
     4921 atctggaacc cgaagacgaa gcgcgtggcg ctcaggtcgc tcgcatcgta gcaatggccg
     4981 gccgtggcga tcaggtcggg gccgaccagg aacccggtgc agaaggcccc cactggctgg
     5041 ccggcaaacg gctcgccgct gcacggcggc aagttgctgt agcgccagct ggacaagctg
     5101 agcgtatagg tgccgtcgcc gttgttgacg acgcggctgg aagagaacag gccgcaggtg
     5161 gcgcgcgcca gccgctgccg cagcggatcc tgctcctcgt agacgtcgat gcgatcgtcg
     5221 ctgccgtaga tgacctgggg ccggatcacc ggcggcggcg gcgtccacgg ttcttcgctg
     5281 atttcggtgc gtcccgcgtg gcgagggggc ttgtccgccg gggttgattc agctcgtacg
     5341 gcgggcgcaa cggctacccc gagtatcagc cagagcagtg cggccggcca gcttaggtgc
     5401 ggtgcgatca acgacaggag ccttcccggc gaatccgaac agcgagtcat gcatcaactg
     5461 acaccgccgc ggcccaaacc ttttcacccc cgggcagggc gaggggactg ccgccattat
     5521 atatcggatc gccggttgcg cgtcaggaat actgcacgaa gaggtccttc agcgccgggt
     5581 agagccgccg gtagcgctga tagcgctgtt cataatcgcg aacatcgcgg cggcgcgggc
     5641 gaacgcgatc accgacccgg atcgcccggc gggccgcttc gttgaagtct ttgtaaaggc
     5701 cggcgccgac gcccgccagc agcgccgcgc ccatcgcgcc tccctcggga ttctccaccg
     5761 ggcggaccgg ctggccgagg atatcggcca gattctggag ccagaacggg ttgcgcgccc
     5821 cgccgccggt cacgcggatg tcgttgatcg ccagcccgcg ggcgcgcatc agctcgagtg
     5881 aatcgcgcaa ggcgaagttc accccctcga tcagcgcgcg cgtcaggtgc gccttctggt
     5941 ggcgcgtgga caggccgaag aacaccccgc gcgcgttggg gtcgggatgc ggcgtccgtt
     6001 cgcccatcag gtaggggaga aaccacagat cctccgcgcc ggcgggcgtt tgcgccgcgc
     6061 gctcgagcag caggtcgaac agattgcgcc gggccttctt cgcctcggcc gcttcggcct
     6121 gacacaatgc gtcgccaaac cactgcagcg agcccgccgc gctgatgacg cagcccatca
     6181 gataccggct gccggcgacg gcatggtcga agctggccag cccgtgctcg cccccgaggt
     6241 gcaccgcgcg ggtcggcgcc aggacaacgc ccgaggtgcc caggctgacc atcgcctgcc
     6301 cttccacgaa cacgccgaca cccaccgccc ccaccggctg gtcgccgccg ccggcgatga
     6361 ccggcgtgcc cgcaacgagg ccggtggcgc gggcggccgc gggggtcagc tcccccgcga
     6421 tctcggccga accgatcagc gggggcagca ccgcggcgtc cagccccagt tgcgcgagga
     6481 tctcgccggc ccatctgcgc tcgccgatca ccttcatcac cgtgccggcc gcgtcggatt
     6541 cgtccatcgc ggcgcggccc gtcaggcggt agcgcaggta gtccttcggc agcagcagtg
     6601 tgcgcagccg gcggtagttc gccttttcat tctgcttgag ccagaggagt ttcggcagcg
     6661 tgaaactggt cagcggcagg ttgcgcagca cccgcaggag cggttcgcgt ccgaccgtcc
     6721 ggatgatttc ctcgcactgc ggcgccgtgc ggccgtcgtt ccagagcagg cagggccgga
     6781 tgacctcgcc ccgtgcatcg agaaagaccg aaccgtgcat ctggccggag aaggagatgc
     6841 cggcgacgcg ccggggatcg atgcggcggc tgacggcctt catcgcctgg gtcgcggccc
     6901 gccaccacgc cgacgggtcc tgttccgacc agttgggccg gggctgcgac agcgcgcagg
     6961 ggcgcgcggc cgaagccagc gcccgccccg cgcggtcaaa cgccaccacc ttgatcgagg
     7021 aagttcccag gtcgacgccg atgagtatct cgcgcagcat cgcgtccgcc ccccatttct
     7081 agaattcgtc ctcgtcctcg tcctcgtgat cggtcgttgc tcctcttgcg ggagggacat
     7141 aaggaacata aaagacataa aggacacaaa aggaactagg attccgtggc gccgtcaggc
     7201 cagcagccgg atcggcagcc gggcttcggt gacctgaccg ccgacgcgct cgacgcgata
     7261 ggcgccggcc tcggggcggg cggcgacggc ttccatgatc cgtgtcccct caaagtgcag
     7321 cgccgcgtcg tcgtcgagcg catagcccgc cgggaagcct tcggcgatca gccgctgata
     7381 cagcggccgg cgctgggcct cgccgtcgta atgcgggcaa tggctgccgg gcaggaagcc
     7441 gagcccatcg tgcagcggcg ccaattggaa cagattgaac gagtcggtcg ttccgcactc
     7501 gaaccagcag agcgagccgg cgcttccgcc ggtgaggatc acgcccgctt cccacgcctc
     7561 gcgcaggatg cggtcgacgc cgtggacgcg ccagacggcg agcatgttgg ccgtgttgcc
     7621 gccgccgacc cagatgatgt cctgnnnnnn nnnnaagggc caggtgcgtg cgctcggcgc
     7681 ggctggccgg gatgcggttg tacatcccga gcagagcgtc ggcggcatcg cccatcgccg
     7741 tgggaatgaa ggcgacgcgt ggccgctgct tgccggtcag gtcgagcaga tagtccacca
     7801 gggggtgggt gcgtccgggg gagggcctca ggccgccgaa ggggatgatg cgccggggtt
     7861 tcgccatcgc ggtcgggggc taccgcggcg gcgccggcag cgggcagtcg ttgttcatgc
     7921 cgtagatatg gcgcaggccc cagtgggtca gctcggggac gacggcgcgc aggaagggga
     7981 ggtgctggcg cagattgagg gcgtcgccgc cggtggcgat gaccgggcag gcctcgccga
     8041 tttcctcgca cgagcggtcg acgagcgtgg tgatcgctcc caggtagccg tgcatgatgc
     8101 cggcctggat gctctcggcg gtggtgcggc cgatgacgcg gcgcggctcg tgcagcggga
     8161 cctgcggcag acgggcggtc ccgcgcgcca gcgcctcgac ggccatctcg atgccgggca
     8221 ggatcgcccc gcccttgtag atcggcaggt gttcggcggt tggagcgggg gccaggtagt
     8281 ccagcgtgat cgccgtgccg aaatcgagga ccatcaccgg cgggccgtag aagtaattgg
     8341 cgccgaccgc gttggcgagc cggtccatgc cgacctcgct gggatgctcg taaccgttgg
     8401 tgatgccgat cggcgtgtcg cggtcgagca ccatcggcgt ggtcttgaac cctttctcga
     8461 tggcgcggat gaccggctgc tccagccgcg ggacgaccga ggagatgatg gcgccgaaca
     8521 tcggcatgtc ctcgccgatc atgccgctca tcaatagcgc atactcgtcg gccgtgcgcg
     8581 gcgggtacga ggcgatcgac cacgtctggc gcagcgcgcc gcggatgaac agccccatgt
     8641 ggatgtgcgt gttgccgata tcgacggtca ggatcatgga ggagtgtccc gagtgctgaa
     8701 cgggcggtcg gttcgaggtt cgaaggggcc ggacgcatcc cccgcggccg agcgatccgc
     8761 ccgaagaatc gtagcgagcc gggaggcgag gcgcaaggtc aagctccg
//
LOCUS       DAOPCO010000165         8791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7490_length_8791_cov_4.681777, whole genome shotgun sequence.
ACCESSION   DAOPCO010000165 DAOPCO010000000
VERSION     DAOPCO010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_8791_cov_4.681777"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2029
                     /locus_tag="PLS90_11435"
     CDS             <1..2029
                     /locus_tag="PLS90_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK03059.1"
                     /translation="GRAWMTGAFTCHFTFMYDLSFYDPEAGEYTLDSFLADGEREFGG
                     YDILLLWHAYPRIGLDQRNQLDFYRDMPGGLEGLRELVNDAHDRGVMVFINYNPWDVS
                     THREGMTDEEMLAALVGEIGVDGIFLDTMSGDSPVLRQKLEEVRPGISLAPENNLTVE
                     SLSICNGSWAQWLGDSDCPPGLEHRKWLIPRHMRWQIARNNYTHRDEIRRAFFNGSGM
                     MIWENIFGVWNRWNASDRRVWRRAAAILHEYQENFASDAWEPFYPAYPVPSEGPDALY
                     KTDGIFINRWPGERATVYTLYKKEPDVSPHSWYGRSFWHQATYVPLFDVPYEPGVQYY
                     DLWNGRPARTERQGDKVVIHGFLDRLSGLGCIAAVPEAKMDEAFNAFIEKQRLEADQI
                     PVADDLRGGIESPLEIRPFEHTEPGRLDQPPQGMVAVPGGPVRVQVTHQRRECGCYPD
                     PGTPADQWEKFTHGTPHNGQVRHDYTVDVAPFLIDEAAVTNAEFKRFLDESGYQPRHT
                     KNFLKHWPNGQMPAELADHPVVYVDLDDARAYAKWAGKRLPTEEEWHLAAQGTDGRKW
                     PWGNEEPDADRANTTGATLPVRDLPASRSPYGCYQMSGNVYEWTESERSDGHTRFCII
                     RGGSYYSATGSDWYMDGGPRPCDHHAKFIMMWPGVDRCSTIGFRCVKDVAS"
     gene            2078..3109
                     /locus_tag="PLS90_11440"
     CDS             2078..3109
                     /locus_tag="PLS90_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HPK03060.1"
                     /translation="MTQSVLLGLLIVVLAAVFQGSFMVPMAYARGWKWENSWLVFSVL
                     GMVVFNWLFARMLVPSLGEIFAAATFGDLVVPALSGLLWGIGAVCFGLGMAAVGFALG
                     YAFIMGLVLSLGAFIPMVVNHPEEIASAKGLLILLGLATMIGGIAISGLAGIRKEREQ
                     GARTGEITKTTNLSMKIGLVICVFAGIFSCLPNIGFDMSRGLMSVALEHGAPEQWAGN
                     VIWAVLFTFGGLANLIYCGYLLKKNGTAGAYGGAGMGRNLGLLALMSAMWIGSFVLYG
                     MGARMMGAWGTVIGWSVFIALAIAVGALWGIAQGEWRNTSAATRRLMLGGVAVLVLAI
                     FVFAYSGSL"
     gene            3134..4081
                     /gene="rbsK"
                     /locus_tag="PLS90_11445"
     CDS             3134..4081
                     /gene="rbsK"
                     /locus_tag="PLS90_11445"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPK03061.1"
                     /translation="MQKILVIGSYNVGLTVLGSRIPRPGETVLGDRFDMGPGGKGSNQ
                     AITIARLGGDVTFLAKLGRDIFADDALRLFEREGLDAKHLLFDPEAHTGAGIIFVNAE
                     GQNAIGVAPGANYRLSTADLDAAEALFTQSRYLLMQLETPLPVVYHAIEKARAAGATV
                     ILNPAPAQPIEPRYLAKIDILTPNETEAEILTGVAVNDVPTAFEAARRLRDQGVKQTI
                     VTLGEKGSVLVEATRETHFPAPKVRSVDTTGAGDAFNGGLVYALADGRTIDEAIGFAS
                     RVGAYSVMSVGVVPGLPRQADLERFEREGGHVGAHVDAS"
     gene            4053..5159
                     /locus_tag="PLS90_11450"
     CDS             4053..5159
                     /locus_tag="PLS90_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK03062.1"
                     /translation="MSGPTLTHRERVERALAHETTDRPPRDFAAEPDVWRRLRAHYRT
                     DSHEEILRAFDTDCRIVSYDWEAFFRPPDTPADAPRAHRAWKRLLASDSYLDPWGARR
                     TITVNEYSTYEELSDYPLAGAESVEDLARYAWPSPDWWDFSQLRAVIERVNPNREYHL
                     RWRCGSIFETAWSLRGFDRMLLDLVVAPDIAGYIMDRILEIHLENLARVMAAAGDLID
                     MVYTYDDVASQQALLMSADMWGQLIRPRQERLFAAAKGYGKPLMLHCCGAVAPLIDEL
                     IDLGLDVLNPVQPLAAGMDLERLKERYGDRLTFHGGIDIQELLPRGTPEQVRREVERV
                     TGILGRDGGYILAPAHHVQADTPVENVLALYGLS"
     gene            complement(5266..6534)
                     /locus_tag="PLS90_11455"
     CDS             complement(5266..6534)
                     /locus_tag="PLS90_11455"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPK03063.1"
                     /translation="MPLKLLTTRACGLLICLALGLATACSKTPDDAVVLYLRMGSPVE
                     QGIWHEAVTAIEEKYPDIKIKVLNVPYEAYWTKFFTLTASGNPPDIVLMESGFYPGFV
                     KKNALMDLTDRVNASELISLDAYHHTPLEWLQIDERLYGLPCDTAVIIPFFNLDMFEE
                     AGVEVPGPDWTWDDYLEIARKLTRDRDGDGNIDVWGSALPPWELAIWSFGGDSVDDPK
                     NPTRCTFDDPKVIEAIQWIADMRLKHGATIREAVQTAQIDPFQSGQVAITWNGHWAVP
                     EYIARANFKWDVAMPPIGPAGRAIANFGSCFSIPAEAKHPEQAWKVIEFICGGEGARM
                     FAMKNIMTPVFLEVANSPEFHNLNPPDHQKYFIDALDYGRLRPETSAYQELVSMINME
                     LEHVWAGEAGVEEVCRRIATKGTRILNEAR"
     gene            complement(6550..7650)
                     /locus_tag="PLS90_11460"
     CDS             complement(6550..7650)
                     /locus_tag="PLS90_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK03064.1"
                     /translation="MGKKTTSSNSTQVSTKRIAEIVGLARSTVVRALRNDPMIKAETI
                     QRVQLVAQQMGYKHNHIARSLASGKTNIIGCIVPNVANPFFADMITEITTRLAEARRS
                     VLLGVTNDSNAVEEMYLETFMNYRVDGILLATSREQYDQEYHRRLKDAASPVVFIGEG
                     EPIGVDSVRADDETGLYELICFLKQNGYERIAFIGDLEAELHHNWLGRVDGYKRGLLE
                     NNLYYDPQYLCDCPRAADLPAVLDRLMRLPKPPEVVCAGNDRLAIELIHLLHKRGMRI
                     PEDLAVVGFDNISMASEMSPRLTTVDLHIKGITNTAVERLLERIRSRQAGEDLEDAGK
                     YHVRIKPELCIRETTRVLTGNAARRKRPVEVG"
     gene            8032..>8791
                     /locus_tag="PLS90_11465"
     CDS             8032..>8791
                     /locus_tag="PLS90_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK03065.1"
                     /translation="MSEQLPTTESVAAPHIGQGPRIFYFIQFASGGAFFPYLSLYYKS
                     IGLGEQMNILLAATPVLLLVAAPMWGIVADKTRAHKQILVLALVGTMLMAFVMLHIRS
                     FGLMLAAVIAFACLFAPIIPLIDNTTLELLGNRRDLYGRHRIGGTLGWMLSALVSGWM
                     VDQMGLRWAFLGYILLAGLAVPAALRLPVHGGPMGLSLAAGVRAFVGNRRWILFLGAI
                     TTGGFGISLIHQYWAVHLDDLGYSRTFLGVTMLLM"
BASE COUNT         1444 a         2745 c         2989 g         1613 t
ORIGIN      
        1 cggccgcgcc tggatgaccg gcgccttcac ctgccacttc acgttcatgt acgacctgag
       61 cttctacgat ccggaggccg gcgagtacac gctcgattcg ttcctggccg acggcgaacg
      121 ggaattcggc ggttacgaca tcctgctcct gtggcacgcc tatccccgca tcggtctgga
      181 ccagcgcaac cagcttgatt tttaccgcga catgcccggc ggcctcgagg gcctgcgcga
      241 actggtgaac gacgcgcatg accgcggcgt catggttttc attaattata acccttggga
      301 tgtctcgacc caccgcgagg ggatgaccga tgaggaaatg ctggcggcgc tggtcggcga
      361 gatcggcgtc gacggcatct tcctggacac gatgagcggc gattcgccgg tgctgcgcca
      421 gaagctcgag gaagtccggc ccggcatctc gctcgccccg gagaacaacc tgacggtcga
      481 aagcctgtcg atctgcaacg gctcgtgggc gcagtggctg ggcgacagcg attgcccgcc
      541 cggcctggag catcgcaagt ggctcatccc gcggcacatg cgctggcaga tcgcgcgcaa
      601 caactacacg catcgcgacg agatccgccg cgcgttcttc aacggcagcg gcatgatgat
      661 ctgggagaac attttcggcg tgtggaaccg ctggaacgcg agcgaccgcc gggtctggcg
      721 ccgcgcggcc gccatcctgc acgaatatca ggagaacttc gccagcgacg cgtgggagcc
      781 gttctatccg gcctatcccg tgccgagcga gggaccggac gcgctgtaca agaccgacgg
      841 catattcatc aaccgctggc ccggcgagcg ggcgaccgtc tacacgctgt acaagaagga
      901 gccggacgtc tcgccgcact cgtggtacgg ccgctcgttc tggcatcagg cgacgtatgt
      961 gccgctgttc gacgtcccct atgagccggg cgtccagtat tacgatctgt ggaacggccg
     1021 gccggcgcgc accgagcgcc agggagacaa ggtcgtgatc catggtttcc tggatcgact
     1081 gtcggggctg ggctgcatcg ccgccgttcc cgaggcgaag atggatgagg cgttcaacgc
     1141 gttcatcgag aagcagcggt tggaagccga ccagatcccc gtggccgacg acctgcgcgg
     1201 cggaatcgaa tcgccgctgg agatccggcc gttcgagcac accgaaccgg gccggctcga
     1261 tcagccgccc caggggatgg tggcggttcc cggcggtccg gtgcgcgtgc aggtgacgca
     1321 ccagcggcgc gaatgcggct gctatcccga ccccggcacg ccggccgacc agtgggagaa
     1381 attcacccac ggcacgccgc acaacggcca ggtccggcac gactacaccg tcgacgtggc
     1441 gccgttcctg atcgacgagg cggcggtgac taacgccgag ttcaagcggt tcctggacga
     1501 atcgggttac cagccgcggc atacgaagaa tttcctcaag cactggccca acggccagat
     1561 gcccgcggaa ctggccgatc atcccgtcgt gtatgtggac ctggacgatg cgcgcgccta
     1621 tgcgaaatgg gccggcaagc gcctgccgac cgaggaagaa tggcacctgg ccgcgcaggg
     1681 aaccgacggg cgcaaatggc catggggcaa cgaggagccc gacgccgacc gggcgaacac
     1741 gaccggggcg acgctgcccg tgcgcgacct gcccgcctcg cgcagcccct acggctgcta
     1801 tcagatgtcg ggcaacgtgt atgaatggac cgagagcgaa cgcagcgacg gtcacacccg
     1861 gttctgcatc atccgcggcg ggagctacta cagcgccacc ggcagcgact ggtacatgga
     1921 cggcggcccg cggccgtgcg atcaccacgc caagttcatc atgatgtggc ccggcgtcga
     1981 tcgctgttcg acgatcggct tccgctgtgt caaggacgtg gcgtcctgaa ttgcttggaa
     2041 tcgacgaacg gctgaactct tgggaaagga gtcgacaatg actcaatcgg tcctgctggg
     2101 gttgttgatc gtggttctgg cggcggtgtt ccaggggagc ttcatggttc ccatggcgta
     2161 tgcccggggg tggaagtggg agaattcctg gctggtcttc tccgtgctgg gcatggtggt
     2221 gttcaactgg ctgttcgcgc ggatgctggt gccgtcgctg ggcgagatct tcgcggcggc
     2281 gacgttcggc gatctggtcg ttcccgccct ctccggcctg ctgtggggga tcggcgccgt
     2341 ctgcttcggc ctggggatgg ccgccgtcgg gttcgcgctg ggctacgcct tcatcatggg
     2401 cctggtgctc agcctggggg cgttcattcc gatggtggtc aaccatccgg aggagatcgc
     2461 ctcggccaag ggtctgctga tcctgctggg gctggcgacg atgatcggcg gcatcgcgat
     2521 cagcggtctg gccggcatcc gcaaggaacg ggagcagggc gcacgcacgg gcgagatcac
     2581 caagacgacc aatctgtcga tgaagatcgg gttggtgatc tgcgtgttcg ccgggatttt
     2641 ctcgtgtctg cccaatatcg gcttcgacat gagccgcggc ctgatgagcg tcgcgctgga
     2701 acatggcgcg cccgagcaat gggccggcaa cgtgatctgg gccgtgctgt tcacgttcgg
     2761 cggcctggcc aacctgatct actgcggcta cttgctgaag aagaacggga cggcgggggc
     2821 gtacggcggc gcgggcatgg ggcgcaatct ggggctgctc gccctgatgt cggcaatgtg
     2881 gatcggcagc ttcgtgctct acggcatggg cgcgcggatg atgggcgcgt ggggcacggt
     2941 gatcggctgg tcggtgttca tcgcgctggc gatcgcggtg ggcgccctgt ggggcattgc
     3001 gcagggcgag tggcgcaaca cgtccgccgc gacgcggcgg ttgatgctgg gcggcgtcgc
     3061 cgtcctcgtg ctggcgatct tcgtctttgc ctacagcggc agtctgtaac attcaatccc
     3121 agggagagat ctgatgcaga aaatcctcgt catcggcagt tacaacgtcg ggctgaccgt
     3181 gctcggctcg cgcatcccgc ggcccggcga aacggtgctg ggcgaccggt tcgacatggg
     3241 cccgggcggc aagggctcca accaggcgat cacgatcgcc cggctcggcg gcgacgtcac
     3301 gttcctggcc aaactggggc gcgacatttt cgccgacgat gcgctgcggc tgttcgagcg
     3361 cgagggcttg gatgccaagc acctcctgtt cgatccggag gcgcacaccg gcgccggcat
     3421 catcttcgtc aatgcggagg ggcagaacgc gatcggcgtg gcgcccggcg ccaattaccg
     3481 gctcagcacg gccgatctgg acgccgccga ggcgctcttc acccagtcgc gctacctgct
     3541 gatgcagctc gaaacgccgc tgccggtggt ctaccacgcc atcgagaaag cccgtgccgc
     3601 cggcgcaacg gtgatcctga acccggcccc ggcccagccg atcgagccgc ggtatctggc
     3661 gaagatcgac atcctgacgc ccaatgagac ggaagccgaa atcctgaccg gcgtcgcggt
     3721 caacgatgtg ccgacggcgt tcgaggccgc gcgacggctg cgcgaccagg gggtcaagca
     3781 gacgatcgtg acgctcggcg agaaggggag cgtgctggtc gaggcgacgc gcgagacgca
     3841 cttcccggcg cccaaggtcc gttcggtgga taccaccggc gccggcgacg cgttcaacgg
     3901 cggattggtc tatgcgctgg ccgacgggcg gacgatcgac gaggcgatcg gctttgcctc
     3961 gcgcgtgggg gcgtactcgg tgatgagcgt cggcgtcgtc cccggcctgc cgaggcaggc
     4021 cgatctggag cgcttcgaac gggagggcgg ccatgtcggg gcccacgttg acgcatcgtg
     4081 agcgggtcga gcgcgcgctg gcccacgaga cgaccgaccg gccgccgcgt gatttcgccg
     4141 ccgagccgga cgtctggcgc cgcctgcgcg cgcactaccg caccgacagc cacgaggaga
     4201 tcctgcgggc gttcgacacc gactgccgca ttgtctccta tgactgggag gcgttcttcc
     4261 gcccgcccga cacgccggcc gatgcgccgc gcgcccaccg cgcctggaag cgcctgctcg
     4321 cgtccgacag ctacctcgat ccgtggggcg cccgccgcac gatcaccgtc aatgagtatt
     4381 ccacctatga ggaactgagc gactatccgc tggcgggggc ggaatcagtc gaggatctgg
     4441 cgcgctacgc ctggccctcg cccgactggt gggacttcag ccaactgcgc gcggtgatcg
     4501 agcgcgtcaa tcccaaccgg gaataccacc tgcgctggcg ctgcggatcg atcttcgaga
     4561 cggcctggtc gctgcgcggt ttcgaccgca tgctgctcga tctggtcgtc gcgcccgaca
     4621 tcgccggcta catcatggat cgcattctgg agatccatct ggaaaatctg gcgcgcgtga
     4681 tggccgcggc gggcgacctg atcgacatgg tgtacacgta cgacgacgtc gcctcgcagc
     4741 aggcgctgct gatgtcggcc gacatgtggg gccagctcat ccggccgcgg caggagcggc
     4801 tgttcgccgc cgccaagggc tacggcaagc cgctgatgct gcactgctgc ggagcggtgg
     4861 cgccgctgat cgacgagctg atcgacctgg ggctcgacgt cctcaacccg gtgcagccgc
     4921 tggcggcggg gatggatctg gaacggctca aggagcgcta cggcgaccgc ctgacgttcc
     4981 atggcggcat cgacatccag gaactgctgc cgcgcggcac gccggaacag gtgcggcgcg
     5041 aggttgagcg ggtgaccggc atcctcggcc gggatggggg ctacattctg gcgcccgcgc
     5101 atcatgtgca ggccgatacg cccgtggaga acgtgctggc gctgtacggc ctgtcctgaa
     5161 gcgaaagccg cggcgcagga gcggaaaaaa aatccgtcgg atcgacccga tccgacggat
     5221 tctggtttgg aatggggtga acccgcgcgc gcgaatccgc gccggctacc gggcttcgtt
     5281 gaggatgcgc gtgcccttgg tcgcgatccg ccggcagact tcctcgacgc ccgcctcgcc
     5341 ggcccacacg tgctccagct ccatgttgat catgctgacc agttcctgat aggccgaggt
     5401 ctcaggccgc aggcgtccgt agtcgagcgc atcgatgaaa tacttctgat gatcgggggg
     5461 attaaggttg tggaattcgg ggctgttggc gacctcgagg aagaccggcg tcatgatgtt
     5521 tttcatggcg aacatgcggg cgccctcgcc gccgcaaatg aattcaatca ccttccacgc
     5581 ctgctcggga tgctttgcct cggccgggat ggagaagcat gaaccgaaat tggcgatcgc
     5641 ccggccggcc gggccgatcg gcggcatcgc gacatcccac ttgaagtttg cgcgggcgat
     5701 gtactcggga acggcccagt gaccgttcca ggtgatcgcc acctggccgg actggaacgg
     5761 atcgatctgg gcggtttgca cggcctcgcg gatcgtcgcc ccatgcttca gccgcatgtc
     5821 ggcaatccac tggatcgctt cgatgacctt cggatcgtcg aacgtgcagc gggtcggatt
     5881 cttcgggtcg tccacgctgt cgccgccgaa cgaccagatc gccagctccc acgggggcag
     5941 ggcgctgccc cagacgtcga tgttgccgtc gccatcgcga tcgcgggtca gcttgcgcgc
     6001 gatctccaga tagtcgtccc acgtccagtc ggggccgggc acttcgacac ccgcttcctc
     6061 gaacatgtcg aggttgaaga acgggatgat gacggccgtg tcgcacggca ggccgtagag
     6121 ccgctcatcg atctgaagcc attcgagcgg cgtgtgatgg taggcgtcga ggctgatcag
     6181 ctcgctggcg ttgacccggt cggtcagatc catcagggca ttcttcttga cgaaccccgg
     6241 atagaagccc gattccatca acacaatgtc gggcggattg cccgacgcgg tgagggtaaa
     6301 gaacttcgtc cagtaggcct cgtaggggac attgaggacc ttgatcttga tgtcgggata
     6361 tttttcctcg atggcggtga ccgcctcgtg ccagatcccc tgctcaacgg gcgaccccat
     6421 ccgcagatag agcaccacgg catcatccgg cgtcttggag caggcggtcg ccagccccag
     6481 cgccaggcag atcaggagcc cgcaggcccg cgtcgtgagc agtttgagag gcatcagatc
     6541 cgtcccccgt tatccgactt cgacagggcg cttgcgccgc gcggcattgc ccgtcagcac
     6601 gcgagtcgtc tcgcgaatgc acaattcggg tttgatgcgc acatggtatt tcccggcatc
     6661 ttccagatcc tcgccggcct ggcggctcct gattcgctcc aacagccgct cgacggccgt
     6721 gttggtgatc cccttgatgt gcaggtcaac ggtcgtcaag cgcgggctca tttcactggc
     6781 catcgagata ttgtcaaacc ccacgaccgc cagatcctcc gggatccgca tcccccgctt
     6841 gtgcagcaga tggatcaatt cgatggccag ccggtcgttg ccggcgcaga ccacctcggg
     6901 cggtttgggc agacgcatca ggcggtcgag gaccgcgggc agatccgccg cgcgcgggca
     6961 gtcgcacaga tattgcgggt cgtaatacag gttattctcc agcaggccgc gcttgtagcc
     7021 gtcgacgcgc cccaaccagt tgtgatggag ttcggcctcc agatcgccaa tgaacgcgat
     7081 ccgttcgtag ccgttctgct tgaggaagca gatcaattca tacaggcccg tttcatcgtc
     7141 ggcgcggacg ctgtcgacgc cgatcggctc gccctcgccg atgaagacga cggggctggc
     7201 ggcgtccttc aggcgccggt gatattcctg gtcgtactgt tcgcgcgacg tggccagcag
     7261 gatgccgtcg acgcggtagt tcatgaacgt ttcgagatac atctcctcga cggcgttgga
     7321 gtcgttggtg acgccgagca ggacgctgcg cctggcctcc gccaggcgcg tggtgatctc
     7381 ggtgatcatg tcggcgaaga acgggttggc aacgttgggg acgatgcagc cgatgatgtt
     7441 ggttttgccc gaggccaggc tgcgggcgat gtgattatgc ttgtagccca tctgctgcgc
     7501 cacaagctgg acacgctgga tcgtctcggc cttgatcatc ggatcgttgc gcagggcgcg
     7561 caccaccgtg gagcgcgcca ggccgacgat ctcagcaatg cgtttggtgc tgacctgagt
     7621 ggagttggag gaggttgtct tttttcccat tctggttcga cagtcgggca tttccgggag
     7681 gatgcctggg tttctcgctt tcagcttcag cgcgccggcc cgcgggggct ggcgccatcg
     7741 ttcttggttg ggggcggcaa tccgtgaccg ggccgatcgt ggtgcgcccg gcaacacccg
     7801 attttatcat atgataatgc cgacatcgca ggtggtcaag cgccatcaaa agcgagttaa
     7861 acggcatcaa cgggctggcg ggttcgagag gcgaattctg gagagggcct ttgggggcga
     7921 ttacgaggac gagcgacgat tttggtgaaa cggggcattc cctaccttgg cgaatccgat
     7981 tatgatcttg ggatttgctc cccggcaggg agcggcgggc ggtcagcatc ggtgtccgaa
     8041 cagcttccca ccaccgaatc cgtggccgcc ccgcacatcg ggcaaggtcc gcggattttc
     8101 tacttcattc agttcgcctc gggcggcgcg ttttttccgt acctgagtct gtactacaag
     8161 agcatcgggc tgggcgagca gatgaacatt ctgctcgccg cgacgccggt cctgctgctc
     8221 gtggcggccc ccatgtgggg gatcgtggcc gacaagacgc gcgctcacaa gcagatcctc
     8281 gtgctggcgc tggtcggcac gatgctgatg gcgttcgtga tgctgcacat ccgctcgttc
     8341 gggctgatgc tggccgcggt gatcgccttc gcctgtctgt ttgcgccgat catcccgctg
     8401 atcgacaaca cgacgctgga gctgctgggc aatcggcgcg atctctacgg ccggcatcgg
     8461 atcggcggga cgctggggtg gatgctctcg gccctggttt ccggctggat ggtcgaccag
     8521 atgggcctgc gctgggcctt tctcggttac atcctgctgg cggggctggc ggtgccggcg
     8581 gcgctgcgcc tgccggtgca tggcgggccg atgggcctga gcctggcggc cggcgtgcgg
     8641 gctttcgtcg gcaaccggcg ctggatcctg tttctgggcg cgatcacgac gggaggcttc
     8701 ggcatttcgc tgatccatca atactgggcg gttcatctgg acgatctcgg ttattcgaga
     8761 acgtttctcg gcgtcacgat gctgctgatg g
//
LOCUS       DAOPCO010000166         8769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7515_length_8769_cov_5.199220, whole genome shotgun sequence.
ACCESSION   DAOPCO010000166 DAOPCO010000000
VERSION     DAOPCO010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7515_length_8769_cov_5.199220"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..481
                     /gene="yajC"
                     /locus_tag="PLS90_11470"
     CDS             98..481
                     /gene="yajC"
                     /locus_tag="PLS90_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPK03066.1"
                     /translation="MDQEMMAALAWAAQEGAAPASPGGDPRSLIFMFTAIGVVFYFLF
                     IRPQRKEQRKREEMINQLAKGDQVVTIGGIHGTVESVDADKQIVTLAVAPKISMKFNR
                     SAVSTVVRRKGQPAKDEAGKEPGKS"
     gene            559..1368
                     /locus_tag="PLS90_11475"
     CDS             559..1368
                     /locus_tag="PLS90_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK03067.1"
                     /translation="MELERESTVEESPGGSPHLERTRAGGSAAGEGLRGAPEAATPAV
                     LDLSPDRITYQKLRNDPEIIGLIRMADRYLETIGYTDHGLKHLARVSQRAFRLLLDLG
                     LPRREAELAAVAGLLHDIGNLVHRDEHPQSSALMAFTLLKERGMEVEEIATVVGAIAN
                     HDDSIGEPVSNVSAALIIGDKSDVLRTRVRNPRMISFDIHDRVNYAAQRSQLTVEPKR
                     HLITLSLDIDTRISQVMEYFEIFMVRMRMSRRAANFLNCDLQLIINNTQVL"
     gene            1410..4223
                     /gene="secD"
                     /locus_tag="PLS90_11480"
     CDS             1410..4223
                     /gene="secD"
                     /locus_tag="PLS90_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPK03068.1"
                     /translation="MKPISRTRIVIIALVTLVSIWQTWPTARYYLALREKPEVESARP
                     FPSTPEPPEDQIEERARWEAENPEVVAWEAAHPEYVAWEKRTRELQKNAIPLGLDLLG
                     GADVTLVIDREKAIQTELTSLMGRLQADFEGQKMAPRFELAADGNSFTITLENPQADA
                     RKAANILAEYEEVIQGDVSQAALETGTVRIAYQPAEIQRRLTEAIEGARKGVAERIDA
                     LGVTQPRISLQGQDRIRIQVPGEGNPDRLISSVIQPAFLEFRMVHAIHKEYRERGESI
                     WDANGKIRPEIPIPAGAEVVEGKLGQLNEETRQVDYTYQQFIVMREAPLTGNDLRNAA
                     VSFNPTDLRNPIQVALEFKPEGAREFHALTREYAGKGRQLAILLDGVVRSAPELQTEI
                     ADGRAVITGGFTNEEASDLSQILKAGSLPAPLQVESKRTVGATLGVDSIMSGVRALVV
                     GTIVVAAFMILYYGTAGAIAVLALILNVLIILAIMELSRATLTMSGIGGILLTVGMAV
                     DANVLIYERMREEVAAGRPLRQAISTGFNRAFTVILDSNLTTLLSALVLLQFTEGSVF
                     GFALTMTFGLLANLFTGLTVTYTLCALWFMWRGSLSLGKLDLLRDPKIKFLSFRKFTL
                     PLSMLFFFGGLIVIWATGGLNFGVDFEGGVHMEVQFAESAEIGEDEIRGALPALEQVI
                     VQEVVDQPNMFLIDAKLVEAGPGENALVATENLMRAGLGEAFGDQFEVVATAQFGSET
                     GLQFSKMALWVTLLSSIAILIYLSLRFEPAFGVAAVLALVHDMIIVIVLAHLWNVTVT
                     LEVVAALMVLLGFSVNDTIVVFDRIRENTRALPGRSFAEICDLSMNQTLMRTIITSGT
                     TLIAVTALLVLGGESIRDFSKVLFLGVLTGTYSSCLLAAPLVYMWNQHRDNRLQIALA
                     QRKLKKPEAARPVGRTVARRQEG"
     gene            4428..5090
                     /locus_tag="PLS90_11485"
     CDS             4428..5090
                     /locus_tag="PLS90_11485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK03069.1"
                     /translation="MHFLDRYLTPAVKTILLLCLVFYFAPAILDLFKPGFALDFHYRF
                     ALQPEVAIFDLHLWQFVTYMFMHGGLFHLLFNMLALFFFGPAIENRLGTRRFWSFFLI
                     SGVVAAISHVALYYGFHRPTGMGLPAFGVPGLVGASGAIMFVLLAFAAYYPEQEVLLY
                     LAIPVKMKVLIGIIIVLDLIGLGNPGSSVAHIAHLGGLVAGYALLSQYHRDWDIRHWR
                     WR"
     gene            5090..5428
                     /locus_tag="PLS90_11490"
     CDS             5090..5428
                     /locus_tag="PLS90_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HPK03070.1"
                     /translation="MLREDKALHPFWFEEAAEAPGAGRIGLTEAFLESVGEEIVGLEL
                     ARAGDRVRAGETFGFLHTHRRSLDLRAPRSLRIRSINAAAVADARLVRQSPYGRGWLA
                     EVEWDEGSQF"
     gene            complement(5531..8098)
                     /locus_tag="PLS90_11495"
     CDS             complement(5531..8098)
                     /locus_tag="PLS90_11495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03071.1"
                     /translation="MSAIEPRTSYGLVATCWALLGVVLSLQLIGGGLDIPALNWFSAW
                     LDAPPHRSFYYIPLAGGVLLIGTLAIYLRRLDPFAGRRWPIWVFLNLFAFTSFPAAEL
                     VALDPHLTWMSRIAAPAVGVGFPLFVILAEWLLARLFRRLGMSAHDRGWRGAALRFLR
                     TSLRFRPGHLATLRLAGLLYVDADECAAAVRLLDQLGHPRQQDETRVVRAMERCFRSL
                     GEIDRALDCLLRLRELEPATPNIDKRILDDYVRLGRDQEALDLLEQGRLRENMDLLRL
                     RERLNVKLGNYAQALEQIRQIAEQEGAPYDNAIALYRELLEQLPENNEIRIQLAQLML
                     SDVAETRRREGAALLEQVVERDPTRLHLSRQLVRYYSESSQLPRAREHLHRLVDAGDP
                     DPEHYLTYAQLLLDEEEQDRARAILERMIEIVPEDWRGHARLGRLLLRQGDLPGAEAR
                     LRDAEERVPPDADQSLLQSLRNDLARRRAEQAIEQMTSALRERGEDTGRRLELIDALI
                     DLGRADAIIEHCETLLELSPALREIIELKLRKAIDRIQQSYRLSDYLGDLYFQEGRFD
                     DLLALYARMSDKALNPDALLIDGCRRILGRAPDHLAARRMLAQACRNVEDWSGIRAAL
                     EPPMRARNPGLSAEDRALWVEAAFRLGDLEEASNVGLTLIDEMAADETFMLMLIEIQQ
                     RLDDHELAWEVYETALQANPESRTLRKMGDRMEQNRSDYRMRRLEERFRSGEMTAAEH
                     FEKAELHSERGEYEHAIKHYQRAGAEEKLADVAMSKMAVTLCDRGMFDMADEVMSSME
                     LTRETVARHPELLDNTYIVARMLEKIRQPERAVKYYKRIFHLDAAYADVVERLERLS"
     gene            complement(8336..>8769)
                     /gene="rpsD"
                     /locus_tag="PLS90_11500"
     CDS             complement(8336..>8769)
                     /gene="rpsD"
                     /locus_tag="PLS90_11500"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPK03072.1"
                     /translation="GVLERQFIRYYKKADRFRGVTGEVMLQLLEQRLDNVLYRAGLAA
                     TRAQARQLIVHGHARVNGQKVDRPSYQVRVGDQVSISDKVRSGKVYGETAALIEKTGQ
                     RASWIEFNPDAGTAQFLRIPSREDLADIEVNEQLIVELYSR"
BASE COUNT         1484 a         2509 c         3319 g         1457 t
ORIGIN      
        1 gggtttgtgc ccgcggggga aaagcgcgcg aggcgcggga gcaagccgtc cgccggagcg
       61 cggaggaccc caccgaactg caagtgaaag gcttccgatg gatcaggaaa tgatggccgc
      121 gctggcctgg gccgcgcagg aaggagccgc gccggccagt ccggggggcg acccgaggtc
      181 gctgatcttc atgttcacgg cgatcggcgt ggtgttctat ttcctgttca tccgcccgca
      241 gcgcaaggag cagcgcaagc gggaagagat gatcaaccag ttggccaagg gggaccaggt
      301 ggtgacgatc ggcgggatcc acgggacggt ggagagcgtc gatgcggaca agcagatcgt
      361 gacgctggcg gtggcgccga aaatcagcat gaagttcaac cggtcggccg tgtcgacggt
      421 ggtccggcgc aagggccagc cggccaagga cgaggcgggg aaggaaccgg ggaaatcgta
      481 aggccggggg cgccggccgc ggggcgcggc gcggggccgg gcgcgccgag gggctgtttt
      541 cggacgtttg gaagtcgcat ggaactggaa cgcgaaagca ccgtcgagga aagtccggga
      601 ggctcgcccc atctggaacg gacgcgcgcg ggcggttcgg ccgctgggga agggctgcgc
      661 ggcgcgccgg aggcggccac gccggcggtg ctcgatctga gccccgaccg catcacgtac
      721 cagaagctgc gcaacgaccc ggagatcatc gggctgatcc ggatggccga ccgctacctg
      781 gagacgatcg gctacacgga tcacgggctg aagcacctgg cgcgcgtcag ccagcgggcg
      841 ttccggctgc tgctggacct ggggctgccg cggcgggagg cggagctggc cgccgtcgcc
      901 gggctgctgc acgacatcgg caacctggtg catcgcgacg aacatccgca gtcctcggcg
      961 ctgatggcgt tcacgctgct gaaggagcgg gggatggagg tggaggaaat cgcgacggtg
     1021 gtgggggcga tcgcgaatca cgacgactcg atcggcgagc cggtgagcaa cgtgagcgcc
     1081 gcgctgatca tcggagacaa gagcgacgtg ctgcggacac gggtgcgcaa cccgcggatg
     1141 atcagcttcg atatccatga ccgggtgaac tacgcggcgc agcggtcgca actgacggtg
     1201 gagcccaagc ggcatctgat cacgctgagc ctggacatcg acacgcggat cagccaggtg
     1261 atggaatatt tcgagatctt catggtgcgg atgcggatgt cgcgccgggc ggcgaatttt
     1321 ctcaattgtg atttgcaact gatcatcaat aatactcagg ttttgtaggg ccccgcgggt
     1381 ccaagacaga ggacgggaca cactccaaca tgaaaccgat atcacgcacc cggatcgtca
     1441 tcatcgcgct ggtgacgctc gtctcgatct ggcagacgtg gccgacggcg cgctactacc
     1501 tggcgctgcg cgagaagccg gaggtggagt cggcgcggcc gttcccgtcc acgcccgagc
     1561 cgcccgagga ccagatcgag gagcgggcgc gctgggaggc ggaaaacccg gaggtggtcg
     1621 cgtgggaggc cgcgcatccg gagtatgtgg cctgggaaaa gcggacgcgc gaactgcaga
     1681 agaacgcgat tccgctcggt ctggacctgc tgggcggcgc cgacgtgacg ctggtgatcg
     1741 accgcgagaa ggcgatccag acggaactga cgagcctgat gggccggctg caggctgatt
     1801 tcgagggtca gaagatggcg ccgcgcttcg agctggcggc cgacggcaac tcattcacga
     1861 tcacgctgga aaacccacag gccgacgcgc gcaaggcggc caacatcctg gccgaatacg
     1921 aggaagtgat ccagggcgac gtgagccagg cggcgctgga gaccgggacg gtgcgcatcg
     1981 cgtatcagcc ggcggagatc cagcggcggc tgaccgaggc gatcgagggg gcgcgcaagg
     2041 gcgttgccga acgaatcgac gccctgggcg tgacccagcc gcggatctcg ctgcaggggc
     2101 aagaccgaat ccggatccag gtgccgggcg aagggaaccc ggatcgtctg atcagcagcg
     2161 tgatccagcc ggcgttcctt gaattccgga tggtgcacgc gatccacaag gagtacaggg
     2221 agcggggcga atcgatctgg gacgccaatg ggaagatccg gccggaaatt ccgatcccgg
     2281 cgggcgcgga agtggtggag ggcaagctgg gccaactgaa cgaggagacg cggcaggtcg
     2341 attacaccta ccagcagttc atcgtgatgc gggaagcgcc gctgacgggc aacgatctgc
     2401 gcaacgccgc ggtgtcattc aatccgaccg atctccgcaa cccgatccag gtggcactgg
     2461 agttcaaacc ggagggggcg cgcgagtttc atgcgctgac gcgcgagtat gcgggcaagg
     2521 gccggcagct cgcgattctg ctggacgggg ttgtccgctc ggcgccggag ctgcagaccg
     2581 agatcgccga cggccgcgcc gtcatcaccg gcggctttac gaacgaggaa gcgagcgact
     2641 tgagccagat cctgaaggcg ggttcgctgc cggcgccgct gcaggtggaa agcaagcgga
     2701 cggtcggggc gacgctgggg gtggactcga tcatgagcgg cgtgcgggcg ctggtggtcg
     2761 ggacgatcgt agtggccgcg ttcatgattc tctactacgg gacggcgggc gcgatcgcgg
     2821 tgctggcgct gatcctcaac gtgctgatca tcctggcgat catggagctg tcgcgggcga
     2881 cgctgacgat gtcggggatc ggcggcatcc tgctgacggt cggcatggcc gtcgacgcca
     2941 acgtgctgat ttacgagcgc atgcgcgagg aggtggcggc gggccggccg ctgcgccagg
     3001 cgatctcgac cggcttcaac cgggcgttca cggtcattct ggactcgaac ctgacgacgc
     3061 tgctctcggc gctggtgctg ctgcaattca ccgagggatc ggtgttcggc ttcgcgctga
     3121 cgatgacgtt cggtctgctg gcgaacctgt ttacgggttt gacggtgacg tacacgctgt
     3181 gcgcgctgtg gttcatgtgg cgcggctcgc tgtcgctggg caagctggac ctgctgcggg
     3241 atccgaagat caaattcctg tcgttccgca agttcacgct gcccttatcg atgttgttct
     3301 tcttcggcgg gttgatcgtg atctgggcga caggcgggct caacttcggc gtggacttcg
     3361 aggggggcgt ccacatggaa gtccagttcg cggagtcggc ggagatcggc gaggacgaaa
     3421 tccgcggcgc gctgcccgcg ctggagcagg tcatcgtgca ggaagtggtg gaccagccca
     3481 acatgttcct gatcgacgcc aagctggtgg aagcggggcc gggggagaac gcgctggtgg
     3541 cgacggagaa cctgatgcgc gccggattgg gcgaggcctt cggcgatcag ttcgaggtgg
     3601 tggcgacggc gcagttcggc agcgaaacgg ggctgcagtt cagcaagatg gcgttgtggg
     3661 tgacgctcct gtcgtcgatc gcgatcctga tctacctgtc gctgcggttc gagccggcgt
     3721 tcggcgtcgc ggcggtgctc gcgctcgtgc acgacatgat catcgtgatc gtgctggcgc
     3781 acctgtggaa cgtgacggtg acgctggagg tcgtcgcggc gctgatggtg ctgctcgggt
     3841 tctcggtcaa cgacacgatc gtcgtcttcg atcggattcg ggagaacacg cgggcgctgc
     3901 cggggcgctc gtttgccgag atctgcgatc tgtcgatgaa ccagacgctg atgcgcacga
     3961 tcattacgtc ggggacgacg ctgatcgcgg tgacggcgct gctcgtgctg ggcggcgaga
     4021 gcatccgtga cttttccaag gtgctgttcc ttggcgtgct gacgggcacg tattcgtcgt
     4081 gcctgctggc ggcgccgctg gtttacatgt ggaaccagca ccgcgacaac cggctgcaga
     4141 tcgcgctggc gcagcgcaag ctgaagaagc ccgaagccgc ccggcccgtg gggcggacgg
     4201 tggcgcgccg gcaggagggt tgagggggcc ggccatgctg gggtgaggtt gccgggcgac
     4261 gccactcagg agcgtggcgc tcccaggttt cggccggttg cttcattcca tgatgcctcg
     4321 gcgcaggaga ggaacgacgg gcggaagtcg aggacgagga cgaggacgat ttggggagtg
     4381 ggggacgggt atctaagaga gtctgagcaa aggctggcgt gggctccatg cacttccttg
     4441 atcgctacct gacccccgcg gtcaagacca tcctgctgct gtgcctggtg ttctacttcg
     4501 cgccggcaat tctggatctg ttcaaacccg gcttcgcgct cgacttccac tatcgcttcg
     4561 cgctgcagcc ggaggtggcg atctttgacc tgcacctctg gcaattcgtc acctacatgt
     4621 tcatgcacgg cgggctgttc cacctgctgt tcaacatgct ggcgctgttc tttttcgggc
     4681 cggcgattga aaaccggctg gggacgcgcc ggttctggag cttttttctg atttccggcg
     4741 tcgtcgcggc gatctcgcac gtagcgctgt attacggatt ccaccggccg accgggatgg
     4801 ggctgccggc gttcggcgtg ccggggctgg tcggcgcctc gggcgcgatc atgttcgtcc
     4861 tgctggcgtt cgcggcgtac taccccgagc aggaggtcct gctctacctg gcgattccgg
     4921 tcaagatgaa ggtgctgatc gggatcatca tcgtgctgga tctgatcggc ctgggcaatc
     4981 cggggagcag cgtggcgcac atcgcgcatc tgggcgggct ggtcgccggc tatgcgctgc
     5041 tgtcgcagta ccaccgcgac tgggacatcc ggcactggcg ctggcgctga tgcttcgcga
     5101 ggacaaggcc ctgcatccat tctggttcga ggaagccgcg gaggcgccgg gggcgggccg
     5161 catcggcctg acggaggcgt ttctggaaag cgtgggggag gagattgtgg ggctggagct
     5221 ggcccgggcc ggggatcggg tgcgcgccgg cgagacgttc ggatttctgc atacgcatcg
     5281 gcggagcctg gacctgcggg cgccgcgctc gctgaggatc cggtcgatta acgcggcggc
     5341 cgtggcggac gcgcggctgg tgcgccagtc gccgtacggc cggggatggc tggccgaggt
     5401 ggagtgggat gagggatcgc aattctgagc cgagagccga ggacgaggga cgagagccga
     5461 gagccgaatg attggggtgg gccgctgggg gacgaggacg agagacaaag gacgattgtt
     5521 ttgagagggg tcaggagagg cgctcgaggc gttcgacgac gtcggcatag gccgcgtcga
     5581 ggtggaagat gcgcttgtag tatttgacgg cgcgttcggg ctggcggatt ttctccagca
     5641 tgcgggcgac gatgtaggtg ttgtcgagca gttcgggatg ccgcgcgacc gtctcgcggg
     5701 tcagttccat cgacgacatc acctcgtcgg ccatgtcgaa catgccgcgg tcgcagaggg
     5761 tgacggccat cttgctcatc gcgacatcgg ccagcttttc ctcggcgccg gcgcgctgat
     5821 agtgcttgat cgcgtgctcg tattcgccgc gttcgctgtg gagttcggcc ttttcgaaat
     5881 gctcggcggc ggtcatttcg cccgagcgga agcgttcctc gagccggcgc atgcggtaat
     5941 cgctgcgatt ctgctccatg cggtcgccca tcttgcgcag cgtccggctc tccgggttgg
     6001 cctggagcgc ggtttcatag acttcccacg ccagctcatg gtcgtcgagg cgctgctgga
     6061 tttcgatgag catcagcatg aaggtctcgt cggcggccat ctcgtcgatg agggtgaggc
     6121 cgacattgga ggcctcctcc aggtcgccga ggcggaacgc ggcctcgacc cagagggcgc
     6181 ggtcctcggc ggagaggccc gggttgcggg cacgcatcgg cggctcgagc gccgcgcgaa
     6241 tcccgctcca gtcctcgacg ttgcggcagg cctgcgcgag catgcggcgg gcggccaggt
     6301 ggtcgggggc gcggccgagg atgcggcggc agccgtcgat gagcagcgcg tcggggttga
     6361 gcgccttgtc cgacatgcgg gcgtagagcg ccagcaggtc gtcgaagcgc ccctcctgga
     6421 aatagagatc gcccaggtag tcgctcaggc ggtagctctg ctggatgcgg tcgatggcct
     6481 tgcgcagctt gagttcgatg atttcgcgca gggcggggga gagttccagc agcgtctcgc
     6541 agtgttcgat gatggcgtcg gcgcgcccca gatcgatcaa ggcgtcgatc agctcgagcc
     6601 ggcggccggt atcctcgccg cgctcgcgca gggcggacgt catctgttcg atggcctgct
     6661 cggcgcgccg gcgggcgaga tcattgcgca gagactgcag cagcgactgg tccgcgtcgg
     6721 gggggacgcg ttcttcggcg tcgcgcaggc gcgcctcggc gccgggcaga tcgccctgac
     6781 gcaatagcag ccggcccagg cgcgcatgcc cgcgccaatc ctccgggacg atctcgatca
     6841 tccgttcgag gatcgcgcgc gcgcgatcct gctcctcctc gtcgagcagc agctgggcat
     6901 aggtgagata atgctcggga tcggggtcgc cggcgtcgac gaggcgatgg agatgttcgc
     6961 gcgcgcgggg caactgcgac gactcgctgt agtaacggac gagctggcgg ctgagatgga
     7021 gccgggtggg atcgcgctcg acgacctgct cgagcaaggc ggcgccctcg cggcggcggg
     7081 tttcggcaac gtcgctcaac atgagttgag ccagttggat gcggatctca ttgttttcgg
     7141 ggagctgttc cagcaactcg cggtagagcg cgatcgcgtt gtcgtagggc gcgccttcct
     7201 gctcggcgat ctggcgaatc tgctcgagcg cctgggcgta gttgcccagt ttgacgttaa
     7261 gccgctcgcg caggcggagc aggtccatgt tttcgcgcag gcggccctgc tcgagcaggt
     7321 cgagcgcctc ctggtcgcgt ccgagccgca catagtcgtc cagaatgcgt ttgtcgatgt
     7381 tgggggtcgc cggttccagc tcgcgcaggc ggagcaggca gtccagcgcg cggtcgattt
     7441 cgccgagcga gcggaaacag cgctccatcg cgcgcacgac gcgggtctcg tcctgctggc
     7501 gggggtggcc cagctgatcg agcaggcgga cggcggccgc gcactcgtcg gcgtcgacat
     7561 agagcaggcc ggccaggcgc agggtggcga gatggccggg ccggaaacgc aggctggtgc
     7621 gcagaaagcg caaggcggcg ccgcgccagc cccggtcgtg cgcggacatg cccaaccggc
     7681 ggaagagacg cgccaggagc cactcggcga ggatcacgaa aagcgggaag ccgacgccga
     7741 cggcgggcgc ggcgatccgg ctcatccagg tcagatgcgg gtcgagagcg acgagttcgg
     7801 cggcggggaa gctggtgaac gcgaacaggt tgaggaaaac ccagatcggc cagcggcgcc
     7861 cggcgaaggg atcgagacgg cgcagataaa tcgccagcgt gccgatgagc aagacgccgc
     7921 cggccagcgg gatgtaatag aacgagcggt gagggggcgc gtcgagccag gcgctgaacc
     7981 agttgagcgc ggggatgtcg aggccgccgc cgatcagttg gaggctgagc acgactccca
     8041 gtaaagccca gcaggtggcg accagaccgt aggaagttcg gggctcaatg gcggacatgg
     8101 tcagattatg gaagaaacgt caagggcagc caagcggaat tgggcggagg cggaaggggg
     8161 tggagaaggg gcgaagaaag ggtggattgg accacgcagg acacgaatgg acacgaaaga
     8221 agaacgcaag ggacgagggc cggatcctgc gcgaaggggc tgaaaatgga gcggcggccg
     8281 ggagggaggt ccggccgccg cggggggtgg ggcgaggagg gaatgatggg ttctgtcagc
     8341 gggagtaaag ctcgacgatg agctgttcgt tgacttcgat gtcggcgagg tcctcgcgcg
     8401 agggaatgcg caggaactgg gcggtgccgg catcgggatt gaactcgatc caactggcgc
     8461 gctggccggt cttctcgatc agcgcggcgg tttcgccata gaccttgccg gagcggacct
     8521 tgtcggagat cgagacctga tcgccgacgc ggacctgata ggacgggcgg tccaccttct
     8581 gaccgttgac gcgggcatgg ccgtggacga tcaactggcg ggcctgggcg cgggtggccg
     8641 ccaggccggc gcggtagagg acgttgtcga gccgctgctc gagcagttgc agcatgacct
     8701 cgccggtgac gccgcggaag cggtcggcct tcttgtaata gcggatgaac tggcgctcga
     8761 ggacgccgt
//
LOCUS       DAOPCO010000167         8719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7566_length_8719_cov_5.124885, whole genome shotgun sequence.
ACCESSION   DAOPCO010000167 DAOPCO010000000
VERSION     DAOPCO010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7566_length_8719_cov_5.124885"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..695
                     /locus_tag="PLS90_11505"
     CDS             276..695
                     /locus_tag="PLS90_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPK03073.1"
                     /translation="MQVLEHGLTTEERREEGRLAVELEVEMQILIPDDTFTPARFRAT
                     VQNVSNRGVMVEIDYLGYGDYQRMLRLTPYIRLCVKTDQDGESRRITGRLVWLDYHGE
                     GRQGGPCHLGISFDRAKPRKFLLAAADGAFGSEAPHG"
     gene            688..1023
                     /locus_tag="PLS90_11510"
     CDS             688..1023
                     /locus_tag="PLS90_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03074.1"
                     /translation="MGKTCNPPVNEVATNDEWMDYAFIPERNGVTESNYLANLDGEIA
                     AANAELERLEAPFRIGASSDQPGVGASLVDAESGDGIRVLGYRASHRLAHRLKQIGDL
                     LRADAADRD"
     gene            complement(1050..1697)
                     /locus_tag="PLS90_11515"
     CDS             complement(1050..1697)
                     /locus_tag="PLS90_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPK03075.1"
                     /translation="MKARILIADDHEIMRQGLRTLLEKNRGLEVVGEAANGREAVRLA
                     AELSPTIVLMDLTMPDMNGIEATRRILKENENLRVIVLSMHADRQFVSESLKAGASGY
                     LLKNCAYDELIRAIEAISRGQTFLSPQIAGVLVEDYRTRLGAPAIAADSPLSEREREV
                     LQLMAEGKSTKEIAAALHISVKTIETHRQQIMRKLKMRSVADLTKYAIRHGLTSL"
     gene            complement(1694..3178)
                     /locus_tag="PLS90_11520"
     CDS             complement(1694..3178)
                     /locus_tag="PLS90_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03076.1"
                     /translation="MPDWTWQLADPGDALPTRVLIADDDELVGMLLARLLTAEGHECL
                     HVRNGREALEELARSNWDMIFVDLEMPEVDGIGLLKEIAHHGYDVVPIVITGGADLRD
                     VMQTARLGSFDFLLKPMRPSMIRGVTQRVIEHRRAVRRSRILARLVEHWESIFNAWPD
                     IVIVLDPSSRILQANRAICRQLEVSSEQIRGQDAHELLCNGAHPPEECIFRSSSVSGP
                     AEPFEFQRPDWGLEFLMTPVALLDRDEQPWGTLFIGRNITLEKRAENLRRDYQRQLQL
                     LGSDRMVREERERRRLASLLHDSIGQTLALTSIKLSHLVKTVPPACREDLAAALDLLN
                     EALAATRSLTFEISPPILYELGLEPAISALLEQFEKRHGIGVALHVPHPIPDLPHDLL
                     GSLYIYTRELIVNCIKHAEATEVAVTILAQPGALTLSVADDGKGFTQDAFDPGSRVPG
                     GYGLFNIRETLSLIGGSVEISTSPKGGGCVVLNMPLATAEEPAP"
     gene            complement(3604..5034)
                     /locus_tag="PLS90_11525"
     CDS             complement(3604..5034)
                     /locus_tag="PLS90_11525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPK03077.1"
                     /translation="MSHRIPQNGALAILTIGVTTGHPLRSILDLFRDEVRVRDASDLV
                     DARQAVAADHHDLIALAVDQLDAAAFEDLRALACAGGQPRILVITTAFEPRLVAPFHE
                     LGIHDIFLAEHLKGGLPRLIQNEIRLRQLEQHNRRLRHLLEGQSTCGKLLGSSKPMRQ
                     IYRRIRKVAGLDMPVLISGESGTELIETAEAIHAQSNRPGMGLLMADCRDRDDQTGAF
                     LFGNVGELPEEGAPHPTESIFACAAGGTVVLQNIECLGRVAQERLLRFLMHPFYQGET
                     AATARAIPRLIVTTSVDLAPQVNQGLFLRALYNRISLLHIRIPALRERREDIPLLANA
                     FLREIRDSQSVECSLAGDALLALYQHDWPGNIAELREVLQAAVARTSTPEIGPEALGL
                     QLPSPASAVVEPIPTKIAAPAQPDDEVSFKKAKREFETNYFTRLLEKASGNMALAARY
                     SRVGRPYLYRKMRAIGLDPDAFRQTA"
     gene            complement(5281..5895)
                     /locus_tag="PLS90_11530"
     CDS             complement(5281..5895)
                     /locus_tag="PLS90_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03078.1"
                     /translation="MSLRDHAVLFVDDDANLLQAFRRQFRELACRTLYASSGHEALEI
                     LDRQPVSLIVSDQAMPIMSGLHLMRVVKARYPSIIRFILTGNATLDLAVEAINQGEVA
                     RFFVKPCSLPELAQAIQQALEKADKPPTMARPARDQAVHPSEYDKRTTGLMHVERSGD
                     GAVLLGETGGNLRDFVRRMQQQDAGAQTATESPAANSPHTTESR"
     gene            complement(5925..6884)
                     /locus_tag="PLS90_11535"
     CDS             complement(5925..6884)
                     /locus_tag="PLS90_11535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPK03079.1"
                     /translation="MTDQPPIPVFFIDDDVRLLDSVRRMLRRAAVSWPAEFFDDPAAA
                     ISAIEDQEHALIVTDWHMAGIDGLEVCQRAHRQRESTQSGRYVILLTGKSETERVVEA
                     LEAGADDYIRKPFELRELLARIQVGLRILHLQRELHEANRLLRVQAATDPLTGLATRR
                     HANELLERELDRVWRGMQALSVLLIDLDKFKAINDGEGHGAGDKVLRQVALRVRANCR
                     RYDTVARWGGDELLVICPHAAAAQAGRVAQRIRKDVDELRIAFNGKALRLTTSIGVAC
                     AEPGHCETIEPLVARADEALYRVKARGGNGVCQAEPRCEATGS"
     gene            complement(6939..8162)
                     /locus_tag="PLS90_11540"
     CDS             complement(6939..8162)
                     /locus_tag="PLS90_11540"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03080.1"
                     /translation="MTTPTAHVLFVDDDPNILRGLRRMLRSMQSQWTMDFVPSGQEAL
                     ELLAQRPVDVLLSDMRMPRMDGATLLHEVRRRHPGVIRIILSGHSDSNLILHSLGATH
                     QFLVKPCDPDRLKVTIERALALKRHALSPALRQTVTRLDTLPVTPADHQELVQAIIAP
                     DVSLQRIAQIVSREVGMTAKIMQLVNSSFFGIAHRVGTAAHAVALLGHDVIRTLVLGT
                     QVFSRDYLDASPEFDAAGFRRHSQQVAALASRLAAQMGCDQHLIDLCYLSGMLHDVGR
                     LLLAAEQAPLFADCLRRARTEHVALDTVEQEILGVSHAALGAALLRVWGFGDPVTEAV
                     EHHHQPRRGGRAEASPLAFMHVADALLSQTGRFPHAPDEPYLAELGWTALLPAWQTLA
                     QEMRRREPARAARVA"
     gene            complement(8170..>8719)
                     /locus_tag="PLS90_11545"
     CDS             complement(8170..>8719)
                     /locus_tag="PLS90_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006474796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03081.1"
                     /translation="QKVLVDLNRAIESTATVSRSEWKYVAELTTDLDPALPPVACYPG
                     ELNQALLNLIINAAQAIGERKRDTTCGKGSIHITTRYLKDADQVEIRIRDDGGGIPEE
                     IRDRIFDPFFTTKPIGKGSGQGLPIVYSVIHDKHGGQVDFVSCVGEGSVFKLRLPVAK
                     MEDPAEVVAERAPLPDAATFPA"
BASE COUNT         1518 a         2819 c         2853 g         1529 t
ORIGIN      
        1 gaggcgatcg ccgcgctgcc gggactggtc cagggactga cgacgagcaa cgccgtcgtt
       61 gcgctcgcgc cgacgcgcga ccgctcgccg cgcggccgag ccacatcgga tcagcatcgc
      121 cggctgttcg gtttacagcg cagctcagct ttcaactgag ggctgtcgcg tcccaagtca
      181 ggaaccccgc atccggccag gatgccataa aaatgcagag tccccattta acgggtcatg
      241 acggaagcag catcggccgt cgaaggagcg caggcatgca ggtcttggaa cacggattga
      301 caaccgagga gcgacgcgaa gaggggcgcc tcgccgtcga gctggaggtg gaaatgcaga
      361 ttctcattcc cgacgacacg ttcacccccg ctcgattccg ggcgacggtg caaaatgtct
      421 ccaatcgcgg cgtcatggtg gagatcgact atctcggcta cggcgactac cagcgaatgc
      481 tgcggctgac cccctacatc cgcctctgcg tgaaaaccga ccaggacggc gaaagccggc
      541 gaatcacggg tcggctggtc tggctcgatt accatggcga aggccgccag ggggggccgt
      601 gccacctggg catttcgttc gaccgcgcga aacccaggaa gttcctgctc gcggccgccg
      661 acggggcgtt cggatcggag gctcctcatg ggtaagacat gcaacccgcc ggtcaatgaa
      721 gtcgcgacca acgacgaatg gatggattac gccttcatcc cggagcgcaa tggcgtgacg
      781 gagagcaact atctggcgaa tctggatggg gagatcgccg cggccaacgc cgaactggaa
      841 cgacttgagg ccccgttccg catcggtgcg tcgtccgatc agccgggagt cggggcaagc
      901 ctcgtggacg cggaatcggg cgatgggatc cgagtgctgg gctatcgcgc ctcgcaccgt
      961 ctggcgcatc gactgaaaca gatcggcgac ctgctgcgcg cggatgccgc cgaccgcgat
     1021 taggataaaa ggatcgatcc gggccggagt tacaacgacg tcagaccatg acggatagcg
     1081 tacttggtca ggtcggcgac cgagcgcatc ttcagtttgc gcatgatctg ctggcgatgc
     1141 gtctcgatcg tcttgacgct gatgtgcagc gccgctgcga tttccttcgt gcttttgcct
     1201 tcggccatca gttggagcac ctcgcgctcg cgttcgctca acggcgaatc ggcggcgatc
     1261 gccggcgcac cgaggcgcgt gcgataatcc tccaccagga cgccggcgat ctgcgggctc
     1321 agaaaggtct ggccgcgcga tatcgcctcg atcgcccgga tcagctcgtc gtaggcgcaa
     1381 ttcttgagca gataaccgga cgcccccgcc ttcaggcttt cggagacaaa ctgccgatcg
     1441 gcatgcatcg acaggacgat gacgcgcaga ttctcgtttt ccttcagaat ccgccgcgtc
     1501 gcctcgatcc cgttcatatc gggcatcgtc agatccatca gcacgatcgt cggcgacagt
     1561 tcggcggcca ggcgcaccgc ctcgcggccg tttgcggctt ctcccaccac ctccagtccg
     1621 cggtttttct ccagcagtgt gcgcagcccc tggcgcatga tttcatggtc gtcggcgatt
     1681 aggatccggg ctttcacggc gcgggctcct cggccgtcgc gagcggcatg ttcagcacga
     1741 cgcaaccacc tcccttgggc gatgtagata tctcgacgct accgccgatc agcgaaaggg
     1801 tctccctgat gttgaacagg ccatagccgc ccgggacgcg cgatccggga tcaaacgcgt
     1861 cctgggtgaa acccttgccg tcgtccgcca cgctcagcgt cagcgcgccg ggctgggcga
     1921 ggatcgtgac cgccacctcg gtcgcctcgg catgcttgat gcagttgacg atcagttccc
     1981 gggtatagat gtagagcgat ccgagcaggt cgtgcggcaa gtccggaatc ggatggggca
     2041 cgtgcagcgc gacgccgatc ccgtggcgct tttcaaactg ctcgagcaag gcgctgatag
     2101 ccggttcgag ccccagttca tagaggatcg gggggctgat ctcgaacgtg aggctgcgcg
     2161 tggcggcgag ggcctcgttg agcagatcga gggccgcggc cagatcctcc cggcaggcgg
     2221 gtgggaccgt cttgaccaga tgcgagagct tgattgacgt caacgccagc gtttgtccaa
     2281 tcgagtcgtg cagcagggaa gcgagccgcc ggcgttcgcg ttcctcgcga accatccggt
     2341 ccgacccgag cagttgaagc tggcgctggt aatcgcggcg caggttttcg gcacgcttct
     2401 ccagcgtgat gttccggccg atgaagagcg ttccccatgg ctgttcatcg cggtcgagca
     2461 gggcgacggg cgtcatcagg aattccagcc cccaatcggg ccgctggaac tcgaacggct
     2521 cggccggacc ggaaaccgag gaggatcgga agatgcattc ctccggcgga tgcgcgccgt
     2581 tgcacagcag ctcatgggcg tcctggccgc ggatctgctc ggaggatacc tccagttggc
     2641 ggcagatcgc gcgattggcc tgaaggatcc ggctcgacgg atcgaggaca atgacgatat
     2701 cgggccaggc attgaagatg gactcccaat gctcgaccag ccgggccaga atgcgcgagc
     2761 gccggaccgc gcggcgatgt tcgatgacgc gttgcgtgac cccccggatc atcgaggggc
     2821 gcatcggttt gagcaggaaa tcgaaggagc cgagccgggc ggtctgcatc acgtcccgca
     2881 ggtcggcgcc gccggtgatc acgatcggca cgacgtcgta gccgtggtgc gcgatttcct
     2941 tgagcaggcc gatgccgtcg acctcgggca tttccagatc gacgaagatc atgtcccagt
     3001 tggagcgggc gagttcctcc agcgcctcac ggccgttgcg cacatggagg cattcgtgcc
     3061 cttcggcggt gagcaggcgg gcgagcagca tgcccaccag ctcatcgtca tccgcgatca
     3121 ggacgcgggt ggggagggcg tcgccggggt cggcgagctg ccaggtccaa tcaggcatct
     3181 gggtgacatc gaccattctc aggaagcggc gccgccccgc gccggagact ccgaaaacct
     3241 gcttggaatc ttaagcatcc ttcgaaggaa gaaggtaacc tatcgatatc tccgatacaa
     3301 cgaggaattt gtaaagctcg ggggcccttc agcgcgccgg gaggcgtccc ggttcttcct
     3361 ctgcctgcgt ctcactcgaa aagctgttcc ctattctcgg gatcgcttga agtcggttcc
     3421 gccgtccgta cgggactctg tcgcgatggg tacccggttc ccgccactga agtcgcgggc
     3481 tgaagtctgc tgaaagatcc ggtaaggacg gggccgtttg aatcggacgt tcacccccat
     3541 tttcatcgcg cgttgtgaac cgcagattca ggagtaactc ggacccaaaa tcgcccctcc
     3601 ggatcaagcc gtctggcgga aggcgtcggg gtccaggcca atcgcgcgca tcttgcgata
     3661 caggtagggc cggccgactc ggctgtaacg cgcggccagg gccatattgc cgctggcttt
     3721 ctccaacagc cgggtgaagt aattcgtctc gaactcccgc ttcgctttct tgaacgagac
     3781 ctcgtcgtcc ggctgcgccg gcgcagctat cttcgtgggg ataggctcca cgacggccga
     3841 cgcgggagag gggagttgca gccccagagc ctccggtccg atttccggcg tgctcgtccg
     3901 ggcaaccgcc gcctggagga cttcccgtaa ttccgcgatg ttgccgggcc agtcgtgctg
     3961 atacagcgcg aggagcgcgt cgcccgccag cgagcactca accgattgcg aatcgcggat
     4021 ctcgcgcagg aaggcgttcg ccagcagcgg aatgtcctcg cgccgctcgc gcaacgcggg
     4081 aatccggatg tgcagcaggc tgatccgatt atagagcgcg cgcagaaaca acccctgatt
     4141 gacttggggg gccagatcga cgcttgtcgt gacgatcagc cggggaatcg cccgcgccgt
     4201 ggcggccgtc tcgccctggt agaagggatg catgagaaac cggagcagcc gctcctgggc
     4261 gacgcggccc aggcactcaa tgttttgcag cacgaccgtt ccgccggcgg cgcaggcgaa
     4321 gatcgactcg gtcgggtggg gcgccccttc ctcgggaagc tcgcccacgt tcccaaacag
     4381 gaaggcgccc gtctggtcgt cacgatcccg gcagtccgcc atcagcaggc ccatgcccgg
     4441 acggttgctc tgggcgtgga tcgcctcggc ggtttcgatc aattccgtgc cggactcccc
     4501 cgagatgagg acgggcatgt cgagtcccgc gaccttgcgg atgcgccgat aaatctggcg
     4561 catcggcttg gagctgccca aaagcttgcc gcaggtgctc tggccctcca gcagatggcg
     4621 caggcggcga ttgtgctgct ccaactggcg cagccggatt tcattctgga tcaggcgggg
     4681 caggcccccc ttcaagtgct cggccaggaa gatgtcgtgg atgcccagtt cgtggaacgg
     4741 agcgaccagc cgcggctcaa atgccgtggt gatgaccagg atgcgcggtt gccccccggc
     4801 gcaggccagc gcgcgcagat cctcgaacgc agcggcatcc agctggtcga ccgccagcgc
     4861 gatcaggtcg tggtgatccg ccgcaaccgc ctgcctggcg tcaaccagat ccgatgcgtc
     4921 gcgcacgcgg acttcgtcgc ggaacagatc gaggatcgaa cggagcgggt gaccggtggt
     4981 cacgccaatg gtcaggatag ccagggcgcc gttctggggg attcgatggc tcatgcgtta
     5041 tctccacgaa tgaactcagc aggtgcttca agcctgccac tgagatcatc ggagccctgc
     5101 gcggcgccat ggattggagg atgtacccct tttgagacac ggaactcccc atggagctcc
     5161 ggttttgact gaccggccct cagggattcc ctgatgagtc atgtggggag gaactagcta
     5221 gacgcaaaga atggagggaa cggatcggaa tgacccatcc agccgggccg tgctccggaa
     5281 ttaccggctt tcggttgtgt gaggcgaatt agcggcgggg gactcggttg cggtttgcgc
     5341 gccggcgtct tgctgttgca tccggcggac gaagtcgcgc aggttgccgc ccgtttcgcc
     5401 aaggagcacg gcgccgtcgc cggaccgttc gacatgcatc agccccgtcg tccgcttgtc
     5461 gtattcgctc gggtggacgg cctggtcccg cgccgggcgg gccattgtgg gcggcttgtc
     5521 cgctttctcc agcgcctgct ggatcgcctg ggcgagctcc ggcagactgc acggcttgac
     5581 gaagaagcgc gcgacctcgc cctggttgat cgcctcgact gccaggtcga gcgtcgcgtt
     5641 gcccgtcaga ataaaccgga tgatcgacgg ataacgcgcc ttgaccacgc gcatcagatg
     5701 caggcccgac atgatcggca ttgcctggtc cgagacgatc agactcaccg gttgacgatc
     5761 gaggatttcc agcgcttcat gacccgatga cgcatagagc gtcctgcagg ccagctcgcg
     5821 aaactggcgc cggaatgcct gcagcagatt cgcgtcgtca tcgacgaaga gcacggcatg
     5881 gtccctcaga ctcatcgatc gctctcccac ttgcttcaat gccgttacga tcccgttgct
     5941 tcgcagcggg gctccgcctg gcagacgccg ttgcctccgc gcgccttcac ccggtaaagc
     6001 gcctcgtcgg cccgcgccac gagcggctcg atcgtctcgc agtggcccgg ctcggcgcag
     6061 gcaacgccga tgctggtcgt caaccgcagc gccttgccat tgaatgcgat ccgtagctcg
     6121 tcgacatcct tgcggatccg ctgtgcgacc cggcccgcct gcgccgccgc ggcatgcggg
     6181 cagatcacca gcagctcgtc gcccccccaa cgggcgaccg tatcgtagcg ccggcagttg
     6241 gcccgcacgc gcagggcgac ctggcgcagc accttgtcgc ccgccccgtg accttcgccg
     6301 tcgttgatcg ccttgaactt gtccaggtcg atcagcagca cgctgagcgc ctgcatgccg
     6361 cgccagaccc gatcgagttc tcgttccagc aactcgttgg cgtggcggcg cgtcgccagc
     6421 cccgtcagcg gatcggtggc ggcctggacg cgcagcaggc ggttggcttc gtgcagttcg
     6481 cgctgcaggt gcagaatccg caggccgacc tggatgcggg cgagcagttc gcgcaactcg
     6541 aacggtttgc ggatgtagtc gtcggcgccg gcctccagag cctcgacgac gcgctcggtc
     6601 tcggacttgc cggtcaacag aatcacatag cgcccggatt gggtggactc gcgttgacgg
     6661 tgcgcccgtt ggcagacctc cagcccgtcg atgcccgcca tatgccagtc ggtcacgatc
     6721 agggcgtgct cctgatcctc gatggccgag atcgcggcgg cgggatcatc aaagaactct
     6781 gccggccagg aaaccgcggc gcggcggagc atgcgtcgca ccgaatcgag cagccggacg
     6841 tcatcgtcga tgaagaacac cgggatcggc ggttgatccg tcatgcgctg gctcccatcc
     6901 aggattgaga ttgatcatgc acggcgtcgc gcgccgaact acgccacccg cgccgcgcgc
     6961 gctggctcgc gccgccgcat ctcctgcgcc agcgtctgcc aggccggcag cagcgcggtc
     7021 cagcccagtt cggcgaggta cggctcgtcc ggggcgtgag gaaaccggcc ggtctggctg
     7081 agcagcgcat cggcgacatg cataaaggcc agtggcgacg cctcggcgcg gccgccccgg
     7141 cgcggctgat ggtgatgctc gaccgcctcg gttacgggat cgccaaagcc ccacacacgc
     7201 aacagggcgg cgcccagtgc ggcgtgcgaa acccccagta tctcctgttc gaccgtatcg
     7261 agtgccacgt gttcggtccg cgcccgccgc aggcagtcgg cgaacagcgg cgcctgctcg
     7321 gccgccagca gcaggcgacc gacgtcgtgc agcatgccgc tcaaataaca cagatcgatc
     7381 agatgctgat cgcaacccat ctgcgccgcc agccggctgg cgagcgcggc gacctgctgg
     7441 ctgtgccgac ggaaaccggc ggcatcgaac tccggcgagg catcgaggta gtcgcgcgaa
     7501 aagacctgcg tcccgagcac cagcgtccgg atcacgtcgt gcccgagcag cgcgaccgcg
     7561 tgcgccgccg ttccgacgcg atgcgcaatc ccgaagaatg acgaattgac cagttgcatg
     7621 atcttggccg tcatccccac ctcgcgtgag acgatctggg cgatgcgctg cagcgagacg
     7681 tccggcgcaa tgatcgcctg caccagctcc tggtggtccg ccggcgtgac cggcagcgta
     7741 tcgagccggg tcaccgtctg gcgcagcgcc gggctgagcg cgtggcgctt caacgccagc
     7801 gcccgttcga tcgtgacctt cagccggtcg gggtcgcacg gtttgaccag aaactgatgc
     7861 gtggcgccca ggctatgcag aatcagattg ctgtcggaat ggcccgaaag aatgatgcgg
     7921 atcacgccgg gatgccgccg gcgcacctca tgcagcaggg tcgcgccgtc catccgcggc
     7981 attcgcatgt cgctcagcag cacgtcgacc ggccgctggg cgagcaactc gagcgcttcc
     8041 tgtccgctcg gcacgaaatc catcgtccac tgcgactgca tcgagcgcag catgcgtcgc
     8101 aggccgcgca gaatgttcgg gtcgtcatcc acgaacagga catgggcggt tggcgtcgtc
     8161 atcggttcct cacgcgggga aggtggcggc gtcggggagg ggagcacgct ccgccacgac
     8221 ttcagccggg tcttccatct tggcgaccgg cagtcgcagc ttgaagacgc tgccctcgcc
     8281 gacgcacgat acgaaatcga cctgcccgcc gtgcttgtcg tgaatcaccg agtaaacgat
     8341 cgggaggccc tgaccgctcc ccttgccgat cggcttggtc gtgaagaagg gatcgaaaat
     8401 ccggtcgcga atttcctcgg gaatgccgcc gccgtcgtcc cggatgcgga tttccacctg
     8461 gtcggcgtct ttcagatagc gcgtcgtgat gtgaatcgag cctttgccgc acgtcgtatc
     8521 gcgttttcgt tcgccgatcg cctgggccgc gttgatgatc agattgagca acgcctggtt
     8581 cagctcgccg gggtagcacg cgaccggcgg cagcgccgga tccagatcgg tcgtcagctc
     8641 ggcgacgtat ttccattcgc tgcgcgagac cgtggcggtg ctctcgatcg cgcgattgag
     8701 gtccaccagc accttctgg
//
LOCUS       DAOPCO010000168         8637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7635_length_8637_cov_5.204847, whole genome shotgun sequence.
ACCESSION   DAOPCO010000168 DAOPCO010000000
VERSION     DAOPCO010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_8637_cov_5.204847"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLS90_11550"
     CDS             <1..918
                     /locus_tag="PLS90_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK03082.1"
                     /translation="IGQGKLHNPAIVAGNHGHEVAGLYACLAMIDTLLNDASPTGENV
                     EGWADAILERCTVHFIPFLNVDNGVRYRELIPTCDYSDRYSRDAEDWQRYIEEFNEPR
                     YYFEKKGVHVPMYGFTPEQVEEWQATGKPLGIRYNDQGIDLFCDYKTFFAPETRAARD
                     YLISQMPCAVLELHNHEPASQVFVPVVGATTNDGLVQLSYGEEMMTRLAERGLPCSQH
                     SVRTYNRYYSQEYLQFPEFVYQRITKLSLFAQINTGYLNDYYRDMLRREPLFRRDGDM
                     KTLTRAQRIQTGWSWIHTFLDFGVQRGFK"
     gene            915..3956
                     /locus_tag="PLS90_11555"
     CDS             915..3956
                     /locus_tag="PLS90_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03083.1"
                     /translation="MMPVPREGKLHRSSGGAGLWILGLLLAGAGWGPRAAEVAPPPIP
                     PEAWWPAVNGEGNHGALGQTGGALTNPTVITELALPPARQMTPLAVKVGTAPAREGSW
                     GATAPSVTQDFDGDGASERVRAVNEPKRVVIEVQQTSDDQISIRRPLDTTHRPGLFLF
                     EPTVADLSPDAPGDEVIFFGYWIDKQRKAADDGILGYACSFSPALGELLWEREFEEIL
                     YFPTTIVDDVNADGRHDLIAAVHYRVLVLDGATGEPLFNVRFSKGRNYALLETFQLDD
                     DPYREILISGDFYSETTVIDLGADAAGNVVARPLWTMLFEHRIEQRLVNTYAQPEMIQ
                     IAPDGEPLLFLNFFNMEGHAIEPVDGFENPLGDIDGDGVCDGENVLGREPFYERGDGR
                     WRTLALSAETGHVRGSIDDQWFEGGHDLNGDGQPELFMLEANGQVRPEFGRVALWTWS
                     GREGRRIWAPEEGAGRWVKATAPFQPHKTSRAENPSAVPFVADLDGDGAAEFMILFDT
                     DGDWNGDRLACYTVAAQGDAEPVLKWAFARPGGSLLPAEWQPGAAGASGSLRVHDRSA
                     RVSYTLNEAGEPLGDPAEWAGGAVANISPVLGDFDANGALELVAPNDLWETVIYQYDP
                     LVKEFTESRRLPGHPLYSLNPLVADLEGDGKAEYILAQPGERGESVIVRYDAEGNAIW
                     KRRLPPIAFGPVERGGGGLCGQPVATDANGDGVADLFLTAFRERVINNMESYMVDGAT
                     GEILWHARDVPTTQTYAMTIGSSNLMTTAELTGDDREELVLFSTLYPHLVDAATGELV
                     QPTGLGSDVFGVDDNGVWYMSAVGDDFDGDGAADIYWYYGWHHVLTDAALKPKWYALR
                     QMAAPQHYAATGRLQAGGGVDIVFTASDDEERTWAKVTPGEVVCLDGATGREQWSWAP
                     PDGLMLETSPILVDVNADGLHDVVVVAGGELIALAAPPAGEPEAKVLWRLALPETRPQ
                     PARLAAADAEGDGNPEIFILPTPGSRANLLMIGNADHGGMP"
     gene            3953..5080
                     /locus_tag="PLS90_11560"
     CDS             3953..5080
                     /locus_tag="PLS90_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK03084.1"
                     /translation="MNESTALAMQDSEDSVRVVRDALEFRRPARIPRCNFFLHYPPEW
                     RERFGPEERHTDVSVWVATEGAFFTRARRVKEEGEYVYDVDAWGRLIRSRSDAYFSEI
                     LEVPLGPGVDPDSIEFDSPLLAGRYLQNFGDAAAHDAALAEARRKFHLFAKIGGPFHR
                     TSFVRGEVQFLMDLATDVPLARTLAEKVADHLVAIGLEALRRYDLYDTGIWIGDDMAF
                     NQRPMFSPRIFEEVFLPIYRSMVARFREAGARFVVLHSDGNITPFLEMLVDAGIDAIN
                     PVERRAGMDPYAIRRRFPRLSMIGGMCNTELLVHGPAAAIRDETRRLIELGREGGLVL
                     GTHTISAEVCTEHLAAYVETLDEEGVLEAEDAPRLKARQLG"
     gene            5187..6035
                     /locus_tag="PLS90_11565"
     CDS             5187..6035
                     /locus_tag="PLS90_11565"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK03085.1"
                     /translation="MTVKQSLDIDLFNERGYVILEGLFAEELVNQARAAMEELVDQQA
                     EHLLKAGLIRDRMENEPFERRLYALYRDHLDKAPMSFRKELHSPLLFDLFFNADLLDR
                     VEELLGPEIRLYPNYTARPKLPDFAGTEVLWHQDGGYTESIQGNQEGVAHLRMVNVWS
                     PLVPANRANGCMQFIPGTHRLGPVPHVPKTHYLEVDRAVLDPLLDQAVDIELLPGDVV
                     LFHNLLFHQGQPNHSDHVRWSLDWRYQDATQHTLREAEGHLARSRRHPEEVVQTAEQW
                     GKLHFK"
     gene            6128..7192
                     /locus_tag="PLS90_11570"
     CDS             6128..7192
                     /locus_tag="PLS90_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK03086.1"
                     /translation="MDVEKSYWFTQFEELGDWLAERASRSRRPARVFTIGKTASGRDI
                     PAIEIGDGPMHRPVIIAAQHGTELAGTNGCLAAIDTLLHDQSPTDENITGWADKILKA
                     CTVRFVPLVNIDCAIRFRQHVPTCDYSDAYGTGEEEWERYAGDYNEPRYFLEKQGIQT
                     PRYGFTPEQMEQWRRHSGRGPGFRYNDRGDDLYFDYKTFRAPETQAIRDFLKQVAPNA
                     VLELHNHEHPSRVFAPVVGTTTEHGLMQLAYGEEMMTRLFERGLPGSKHSVRTYTYYT
                     SDDYLQFPEYVYQHVTPMIVFAEITTGYLNDYLRKAMHDDPMNRRDIDCPTVTRPQRI
                     LTSWSWIHTFLDLGVARNFQ"
     assembly_gap    7224..7323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7470..8450
                     /locus_tag="PLS90_11575"
     CDS             7470..8450
                     /locus_tag="PLS90_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPK03087.1"
                     /translation="MSRKVKVAAFTTDYLRTQPRTLEQNLDWTCETIDLLAAEAPDII
                     TLTEAFDCRELDLKCWEKAQVLDGPTFTRMAAKAREHHCYIICSFIERRDGRLYNTSA
                     VIDRRGELVGRYDKIHPTTTQAYDADYDGELEEFITPGRTAPTVLQTDFGAIGCQICF
                     DANWHAGWGELKRAGAEIIFFSSAFSAGRILQSIATLYHLPIVAACRQPRCRIIDRDG
                     LIISRQGDYQRWVCDTLDLDNPLFHLDGQWAKMEEIRKRFGPAIKIRVYEEEGWWRIM
                     PLREDLEIPPIIREYGLQTLEEYFAAAERIQDERRAQPLAAPQAKPVVAR"
     gene            complement(8463..>8637)
                     /locus_tag="PLS90_11580"
     CDS             complement(8463..>8637)
                     /locus_tag="PLS90_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPK03088.1"
                     /translation="RLINYLTTLKMELTTIARACGKSNVHHLEREDLVALTVEAAAMA
                     KRPLAGTNWIPGA"
BASE COUNT         1547 a         2570 c         2994 g         1426 t
ORIGIN      
        1 atcggccagg gcaagctgca caatccggcg attgtcgcgg gcaatcacgg ccacgaggtg
       61 gcggggctgt atgcgtgcct ggcgatgatc gatacgctgc tcaacgacgc gtcgcccacg
      121 ggcgaaaacg tcgaaggctg ggccgacgcg atcctcgagc gctgcacggt gcatttcatc
      181 ccgttcctga acgtggacaa cggcgtgcgc taccgggagc tgatcccgac gtgcgactac
      241 agcgaccgct attcgcgcga tgcggaggat tggcagcgct acatcgagga attcaacgaa
      301 ccgcgctact atttcgagaa aaagggcgtc catgttccga tgtatggctt cacgccggag
      361 caggtcgagg aatggcaggc gaccgggaag cccctgggga tccgctacaa cgatcagggg
      421 atcgacctgt tctgcgacta caagacgttt ttcgcgccgg agacgcgggc cgcgcgcgat
      481 tatctgatca gccagatgcc gtgcgcggtg ctggagctgc acaaccacga gccggccagc
      541 caggtcttcg tgccggtggt cggggcgacg acgaacgacg gcctggtgca gctcagttac
      601 ggcgaggaga tgatgacgcg gctggccgag cggggcttgc cgtgctcgca acactcggtg
      661 cggacctaca accggtacta cagccaggag tatctgcagt ttcccgagtt cgtctaccag
      721 cggatcacca agctgtcgct gttcgcacag atcaacacgg ggtatctgaa cgattattac
      781 cgcgacatgc tgcgtcgcga gccgctgttc cggcgcgacg gcgacatgaa gacgttgacg
      841 cgcgcgcaac ggatccagac gggctggtcg tggatccata cattcctgga tttcggcgta
      901 cagcgaggtt tcaaatgatg ccagtgcctc gcgaaggcaa actccatcgc agttccggag
      961 gcgccggact gtggatactc ggcctgctgc tggccggggc ggggtggggc ccgcgggccg
     1021 cggaggtggc gccgccgccg atcccgccgg aggcctggtg gccggcggtc aacggcgagg
     1081 ggaaccacgg ggcgctgggg cagaccggcg gcgcgctgac gaacccgacg gtcatcacgg
     1141 agctggcgct gccgccggcg cggcagatga cgccgctggc ggtgaaggtg gggacggcgc
     1201 cggcgcgcga ggggtcctgg ggggcgacgg cgccgtcggt gacgcaggat tttgacgggg
     1261 acggggcgag cgagcgggtg cgggcggtga acgagccgaa gcgcgtggtg attgaagtgc
     1321 aacaaacatc agatgatcag atttcgatcc gccgacccct cgacaccacc caccgtcccg
     1381 gcctcttcct cttcgaaccg accgtcgccg atctctcgcc cgacgccccc ggcgacgagg
     1441 tcatcttctt cggctactgg atcgacaagc aacgcaaagc ggccgacgac ggcatcctcg
     1501 gctacgcctg ctcgttctcc cctgccctgg gcgagctgtt atgggagcgc gaattcgagg
     1561 agatcctcta tttcccgacg acaatcgtcg acgacgtcaa tgccgacggc cggcacgatc
     1621 tgatcgccgc cgtgcattac cgggtgctgg tgctcgacgg cgcgacgggc gaaccgctgt
     1681 tcaatgtcag gttcagcaag ggacgcaatt acgcgctgct cgaaacgttc caactggacg
     1741 acgacccgta tcgcgagatc ctgatctcgg gcgatttcta ctccgagacg acggtgatcg
     1801 atttgggcgc ggacgccgcc ggcaacgtcg tcgcccggcc gctctggacg atgttgttcg
     1861 agcatcgcat cgagcagcgg ctggtcaata cgtatgccca gccggagatg atccagatcg
     1921 cgcctgacgg cgagccgctg ctgtttctga atttcttcaa catggaaggg cacgccatcg
     1981 agccggtcga cgggttcgag aatccgctgg gcgacatcga tggcgacggg gtgtgcgatg
     2041 gcgagaacgt cctgggccgg gaaccattct acgagcgggg ggacgggcgg tggcggacgc
     2101 tggcgttgtc ggccgagacc ggccatgtcc gggggtcgat cgacgaccag tggttcgaag
     2161 ggggacacga tctgaatggc gacggccagc ccgagctgtt catgctggag gccaacggcc
     2221 aggtgcggcc cgaattcggc cgggtcgcgc tctggacgtg gagcggcagg gagggccgac
     2281 ggatctgggc gccggaggag ggcgcggggc ggtgggtcaa ggcgacggcg cccttccagc
     2341 cgcacaagac gtcgcgcgcc gagaatccga gcgcggtccc gttcgtcgcc gatctggacg
     2401 gcgacggcgc cgcggaattc atgatcctgt tcgacaccga cggcgactgg aacggcgacc
     2461 ggctggcctg ttacacggtc gcggcgcaag gcgatgccga acccgtgctg aagtgggcct
     2521 ttgctcgccc cggcgggtcg ctgctgcccg cggaatggca gccgggcgcc gccggtgcga
     2581 gcggctcgct gcgggttcac gaccggagcg cccgggtcag ctacacgctg aatgaagcgg
     2641 gcgaaccgct gggcgacccg gcggaatggg cgggcggcgc ggtggccaat atctcgccgg
     2701 tcctgggtga ttttgatgcg aatggggcgc tggaactggt ggcgccgaat gatctgtggg
     2761 agacggtgat ctatcaatat gatccgctgg tgaaggaatt cacggaatcg cgccgcctgc
     2821 cgggccatcc gctttacagt ctgaacccgc tcgtggcgga tctggagggc gacggcaagg
     2881 ccgaatacat cctggcgcag ccgggcgagc ggggcgagtc ggtgattgtc cggtacgacg
     2941 ccgaggggaa cgcgatctgg aagcggcggc tgccgccaat tgcgttcggt ccggtcgagc
     3001 gggggggcgg ggggctgtgc ggccaaccgg tggcgaccga cgccaatggg gacggcgtgg
     3061 ccgatctgtt cctgaccgcg ttccgcgagc gcgtgatcaa caatatggaa agctacatgg
     3121 tggatggggc gaccggagag atcctgtggc acgcccgcga cgtgcccacc acgcagactt
     3181 acgcgatgac gatcggatcg tccaatctga tgacgaccgc cgaactgacg ggggacgacc
     3241 gcgaggaact ggtccttttc agcacgctgt atccgcatct ggtcgacgcg gcgacgggcg
     3301 agctggtcca gcccaccggc ctggggtcgg atgtgttcgg cgtggacgac aatggcgtct
     3361 ggtacatgtc ggcagtgggc gatgattttg acggcgacgg cgccgccgac atctactggt
     3421 attacgggtg gcatcatgtg ctgaccgacg cggcgctgaa gccgaagtgg tatgcgctgc
     3481 ggcaaatggc ggcgccgcag cactatgccg ccaccgggcg gctgcaggcg ggcggcggcg
     3541 tggacattgt ctttaccgcc agcgacgacg aggaacgcac gtgggccaag gtgacgccgg
     3601 gtgaagtcgt ctgcctggat ggcgccaccg gccgggagca atggagctgg gctccgccgg
     3661 acgggctgat gctggagacc agcccgatcc tggttgatgt gaacgccgat gggcttcacg
     3721 acgtggtggt cgtggccgga ggcgagctga tcgcgctggc ggcgccgccc gccggcgagc
     3781 cggaggcgaa ggtcctgtgg cggctggcgc ttcccgagac acggccccaa ccggcgcgac
     3841 tggccgccgc ggatgccgaa ggcgacggca atccggagat ctttattctg ccgacgcccg
     3901 gctcacgggc caatctgctg atgatcggca acgccgacca tggggggatg ccatgaacga
     3961 gagtaccgcg ctcgcgatgc aggattcgga ggacagcgtg cgggtcgtcc gggatgcgct
     4021 ggaattccgg cggccggcgc gcatcccgcg ctgcaatttc tttctgcact atccgccgga
     4081 gtggcgcgaa cgtttcggcc cggaggagcg gcacaccgac gtctcggtct gggtggcgac
     4141 cgagggggcg ttcttcacgc gcgcccggcg ggtgaaggag gaaggggaat atgtctatga
     4201 cgtcgatgcg tgggggcggc tgatccgttc gcggagcgac gcgtatttct cggagatcct
     4261 ggaggttccg ctcgggccgg gggtcgatcc cgattcgatt gaattcgact cgccgctgct
     4321 ggcggggcgc tatctacaga acttcggcga cgcggcggcg cacgacgccg cgctggccga
     4381 ggcgcggcgc aagttccacc tgttcgccaa gatcggcggg ccgttccatc gcaccagttt
     4441 cgtgcgcggc gaggtccagt ttctgatgga cctggcgacg gacgtgccgc tggcgcgcac
     4501 gctggcggag aaggtcgcgg atcatctcgt cgcgatcggt ctggaggcgc tgcgccgcta
     4561 cgacctgtat gacacgggga tctggatcgg cgacgacatg gccttcaatc agcgcccgat
     4621 gttcagcccg cgcatttttg aggaggtgtt cctgccgatc taccggagca tggtggcgcg
     4681 gttccgcgag gcgggcgcgc gcttcgtcgt gttgcactcc gatggcaata tcacgccgtt
     4741 tctggaaatg ctggtggatg ccgggatcga cgcgatcaac ccggtcgagc ggcgcgcagg
     4801 gatggatccc tacgcgattc gccggcggtt tccgcggctg tcgatgatcg gcgggatgtg
     4861 caacaccgaa ctgctggtgc atgggcccgc cgctgcgatc cgcgacgaaa cccggcggct
     4921 gatcgagctg ggccgggagg ggggcctggt gctgggcacg cacacgattt ccgcggaagt
     4981 ctgcaccgag cacctggcgg cgtacgtgga aacgctggat gaagaggggg tactcgaagc
     5041 cgaggacgcg ccgcggctca aggcgcggca attgggctga atcgcccgat cgaaagaatc
     5101 gccccgaaat ggaccgaaac ggccgcaagc tgcctcggat ttctcctcac cggagaaccc
     5161 acaggattgg aaacggagtc aagatcatga cagtcaaaca atcgctcgac atcgatctgt
     5221 tcaacgaacg cggctacgtc atcctagagg gcttgttcgc cgaggagctc gtcaaccagg
     5281 cgcgggcggc gatggaggaa ctggtggatc agcaggcgga gcacttgctc aaggcggggc
     5341 tgatccgcga ccggatggaa aacgaaccgt tcgagcgccg gctctacgcg ctctatcgcg
     5401 atcacctgga caaggcgccg atgtcgttcc gcaaggagct ccattcgccg ttgctgtttg
     5461 atctcttttt caatgccgac ctgctggacc gggttgaaga gcttctcggg ccggagatcc
     5521 ggctctatcc gaactacacg gcgcggccca aactgcccga cttcgccggc accgaggtgc
     5581 tgtggcatca ggacggcggc tataccgaaa gcattcaagg caaccaggag ggggtcgcgc
     5641 acctgcggat ggtgaatgtc tggtcgccgc tggtgccggc caaccgggcc aatggttgca
     5701 tgcagttcat cccggggacg caccggctgg ggccggtgcc gcacgttccg aagacgcatt
     5761 atctggaagt agaccgcgcg gtgctcgatc cactgctcga ccaggcggtg gatattgaat
     5821 tgctgccggg cgatgtcgta ctgtttcata atctgctgtt ccaccagggc cagcccaatc
     5881 attccgacca tgtgcgctgg agtctggact ggcgctacca ggatgcgacg cagcacacgc
     5941 tgcgcgaggc cgagggtcat ctggcgcgca gccgccgcca cccggaggag gtggtgcaga
     6001 cagcggagca gtggggcaag ctgcatttca aatgagaagc cggcccgtgg atggagcgcc
     6061 cgggcggcgc ccggcgcgcg cgggcgaagc tgcatccaac ccttctcgac tggaaaggcg
     6121 aaacgccgtg gacgtggaaa aatcctactg gtttacgcag tttgaggaac tcggcgactg
     6181 gctggccgaa cgcgccagcc gctcccgccg gcccgcgcgg gtattcacca tcggcaaaac
     6241 ggcgagcggc cgcgacatcc cggcgatcga gatcggcgac ggtcccatgc atcgtcccgt
     6301 gatcattgcg gcgcaacacg gcaccgaact ggccgggacg aatgggtgcc tcgccgcgat
     6361 cgatacgctg ctgcacgatc aatcgcccac ggacgagaac atcaccggct gggccgacaa
     6421 aatcctgaag gcgtgcacgg tccgtttcgt cccgctggtc aacatcgatt gcgcgatccg
     6481 cttccgccag cacgtgccca cctgcgatta cagcgatgcg tatgggacgg gcgaggagga
     6541 gtgggaacgc tacgcggggg attacaacga gccgcgctac ttcctggaga agcagggaat
     6601 ccagacgccg cgctacggct tcacgcccga gcagatggag cagtggcgac ggcacagcgg
     6661 ccgcgggccg ggcttccgct acaacgaccg gggagacgat ctctacttcg attacaagac
     6721 attccgcgcg ccggagaccc aggccatccg cgatttcctg aagcaggtcg cgccgaacgc
     6781 cgttctcgag ctgcacaacc atgaacatcc gtcgcgggtt ttcgccccgg tggtcggcac
     6841 gacgaccgag catgggctga tgcagcttgc ctacggcgag gagatgatga cccgcctgtt
     6901 cgaacgcggc ctgcccggct cgaagcactc ggtgcgcacc tatacgtact acacgagcga
     6961 cgactatctg cagtttcccg agtatgtcta tcagcatgtg acgccgatga ttgtcttcgc
     7021 cgagatcacg acgggatatc tgaatgatta cttgcgcaag gcgatgcacg acgacccgat
     7081 gaaccggcgc gacatcgatt gccccacagt gacgcgaccg cagcgcattc tgacctcctg
     7141 gtcgtggatc catacatttc tggacctggg agtggcgcgg aatttccagt aaaataaggc
     7201 gccgtcgagg atccgccggg agtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnggagtgg cgcggaattt ccagtaaaat aaggcgccgt cgaggatccg ccgggagtga
     7381 gattgccgac cctggagggc tagcgcgagc cgattctcag ggggcggttt cgaggacgag
     7441 aggcgaaatt cagagcaagg ggatcgagca tgagccgaaa agtcaaagtc gcggcattca
     7501 ccacggatta cctgcggacg cagccgcgga cgctggaaca gaatctggac tggacatgcg
     7561 agacgatcga tctgcttgcg gccgaggcgc ccgacatcat cacgctgacc gaagccttcg
     7621 attgccgcga actggacctc aagtgctggg agaaggcaca ggtgctcgac ggccccacgt
     7681 tcacccgcat ggcggccaag gcgcgcgagc atcactgcta cattatctgc tcgtttatcg
     7741 agcgtcgcga tggccggttg tacaatacgt cggcggtgat cgaccgccgg ggggaactgg
     7801 tcgggcgcta cgacaagatc cacccaacga cgacgcaggc gtacgacgcg gattatgacg
     7861 gcgagctgga ggaattcatc acgccgggtc ggaccgcgcc caccgtgctg caaaccgact
     7921 tcggcgcgat cggctgccag atctgctttg acgccaactg gcacgccggc tggggcgagc
     7981 tgaagcgggc cggggccgag atcatctttt tttcatcggc gttcagcgcg gggcggatcc
     8041 ttcagtcgat cgccacgctg tatcatcttc cgatcgtggc cgcctgccgg cagccgcggt
     8101 gccggatcat cgatcgcgac gggctgatca tcagccggca gggggattac cagcgctggg
     8161 tctgcgatac gctcgacctg gataacccgc tgtttcacct ggacgggcag tgggccaaga
     8221 tggaggagat ccgcaagcgt ttcggaccag cgatcaagat tcgcgtgtat gaagaggaag
     8281 gctggtggcg gatcatgccg ttgcgcgagg atctcgagat tccgccgatc atccgcgaat
     8341 acgggctgca gacgctcgag gagtatttcg cggcggcgga acggatccag gatgaacggc
     8401 gcgcgcagcc gctggcggcg ccgcaggcca agccggtcgt ggccagatag cgcggccgcg
     8461 gctcaggcgc cgggaatcca gtttgtcccg gccagcgggc gtttggccat cgccgccgcc
     8521 tcgacggtca gggcgaccag gtcctcgcgc tcgaggtgat gcacgttgga cttgccgcag
     8581 gcccgcgcga tcgtggtcag ttccattttc agcgtcgtca ggtagttgat caaccgg
//
LOCUS       DAOPCO010000169         8625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7647_length_8625_cov_5.164294, whole genome shotgun sequence.
ACCESSION   DAOPCO010000169 DAOPCO010000000
VERSION     DAOPCO010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_8625_cov_5.164294"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PLS90_11585"
     CDS             complement(<1..1261)
                     /locus_tag="PLS90_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPK03089.1"
                     /translation="MARDSLRIEVSATLREAVGFGEGDGGLDYFALLGVARDCNDAGL
                     IDKALIERTKALRKWQNSPKFGAEVVKLLNQLHRAVKILKDPNRCALYREELARLERG
                     ETASAEDRFADLVRAAMADAQLDAESRQRLTEFAVREGISSAEAQRIATHVRAEMEQR
                     RAADRQAQPAQEEGWQFRIAVEGEEGFQMMLAGMEASGEVTEEHLRNLLRDAPRYQLD
                     ALRAGKLIADFQARRFKRMVERVAGGRAINDDQARLLVMKARAYGLDDVKAYEIISDY
                     TLSAYSTEDGLSALELTQSFTQDDIAEIIEGRAAGPARRPLWSRLTSAVPAGVRNAVL
                     GVIALGVVVLGVFYLKDVGLGGPPAADEEIVIIPPEEQPPAEPVVSPAETAGTEPAAT
                     PAPIATIEPTEPPRQLVSHPDPPSGMIR"
     gene            complement(1261..2835)
                     /locus_tag="PLS90_11590"
     CDS             complement(1261..2835)
                     /locus_tag="PLS90_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPK03090.1"
                     /translation="MNDFADNAIAVGIDLGTTYSVVGLVDEYGQAQIIPNSDNQRTTP
                     SAVYIDGSSVIVGSIAKEQTTLNPAHTIQFIKPYIGRRRKRFMLSGQEWSPEELSAMI
                     LRKVVRDAEIAMGGQKKITHAVITVPAFFTEQQRKSTEDAGRIAGLNVMSIINEPTAA
                     ALAYGFDTLGADKTVLVYDLGGGTFDVTVMRISGDTVTMLATDGDVQLGGKDWDERLI
                     DYVAEQFLARHGIDPREDYETLQGLYTQAEQAKLRLSKLPATRLAVHLNGTTESYDIT
                     REQFESMTEDLLLQTETTLQLTVQEAGLSFGQIDECLLVGGSTRMRSVRAMLKEVLGT
                     EPKLVLNPDECVAQGAAIHAVMMQLRMLDQGLPVPIPQVREEHLDRFRQMEERLINSH
                     TLGILALNKEGQRVVAPIIPRRAFIPRREVKTFRTSVENQQQIRIVVMEGEGEDPEAC
                     VELGMCLVRGLPSGLPKGTPIEVSFHCSHDGQLSVTARLPTIDQVVQTVIERTMGMSE
                     EAIEASRERLQMMSIE"
     gene            3628..4425
                     /locus_tag="PLS90_11595"
     CDS             3628..4425
                     /locus_tag="PLS90_11595"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPK03091.1"
                     /translation="MTARSLEQIIQRQIHRWNYIAQLLNYHPGRPPGDVPAPREEGGT
                     RHPVLCISRDLGAGARWIAKALCERLGYQLMGSQLIDEVARDLKVQRSLIETLDETTR
                     GAIELMLESQVRGRQIEPGDYLRALYRVIQTIAIKGGVVLLGRGARFIVRDRSALNVL
                     ITASLETRCRRYAEQETCGIREAEARLLRADRDRAEFIRRYFGQDVRDPAHYDLSLNT
                     DRVSAEAAVELILRALELRGYDLSVMAIRDDRTLRAGGRAGASAAVK"
     gene            4497..5063
                     /locus_tag="PLS90_11600"
     CDS             4497..5063
                     /locus_tag="PLS90_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03092.1"
                     /translation="MPDPTPRRSEALSRETRQEILLRLANWEQLGGAVEDRVTAVADD
                     PEWVMLLRWELGLEPLSAEARLLRRHIATLRPCARHGLARTRELIEMIGLKKARDLAD
                     CGCNGEARHYHFGDTHHNELVKLLKQAAAWAEGDWIGAGACGLILGDATPEKRWLVAS
                     LCKQLKTQIRSANPRHAIPIPRHRCSAG"
     gene            complement(5169..6452)
                     /locus_tag="PLS90_11605"
     CDS             complement(5169..6452)
                     /locus_tag="PLS90_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPK03093.1"
                     /translation="MPRAKVAILRTSPATVRSDYHELLNLAEYQQTIARDADTALKVN
                     ISWHFFFPASSTTPWQLDGVIAAMLADGYDKNLIHACHNRTVVIDSHLGERENKQLDV
                     VEKYGLRNIHLYEGEEWIGIKEAVGDLTKKFLCLNEVYPKGFSIPRRFLGENIIHLPT
                     VKTHVFTTTTGAMKNAFGGLLNEHRHWTHPVINETLVDLLMIQKQIHRGVFAVMDGTF
                     AGDGPGPRCMLPHVKNVILASADQVAIDAVAARLMGFDPLVDCRYIRLAHDAGLGCGD
                     PREIEIVGDADAAREDWNFVGPYKKMTFASQMQHKIYWGGLKRPIEWSLKTWLAPWAY
                     VASVLYHDLYWYPARSRKMMDLALRSDWGRLFQNWGRVEENEQGFQNVGQHHPEFQRN
                     LSAMLKQGAKVLGTCLLKAPEIDRRKQKTEPLKDS"
     gene            complement(6752..7264)
                     /locus_tag="PLS90_11610"
     CDS             complement(6752..7264)
                     /locus_tag="PLS90_11610"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPK03094.1"
                     /translation="MSEAPQPDPVATGQIEPRATIRFRPATVEDIDAIHGLIDEASRT
                     TTVLPRKRENICEKLRDFLVGERDGRIIACGALALFSPVLAEIRSLVVAPDCRGQGAG
                     VQLVGALLAEARRIGLHRVFALTDNPPFFERSGFRIVDKHTLPQKVWTDCINCPLYLS
                     CTEEAVEIRL"
     gene            complement(7691..8029)
                     /locus_tag="PLS90_11615"
     CDS             complement(7691..8029)
                     /locus_tag="PLS90_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPK03095.1"
                     /translation="MKMIRAIIQPHRLEAVRDALTQRGIMGMTVFEARGYGRQRGHTE
                     VYRGAEYQVAFRPKIVADLVVPDNQVDDATDAIVSAAKSGSVGDGKIFIFEVGDAIRI
                     RTGDRDENAL"
     gene            complement(8100..>8625)
                     /locus_tag="PLS90_11620"
     CDS             complement(8100..>8625)
                     /locus_tag="PLS90_11620"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03096.1"
                     /translation="ALALVTTHVSAAAGALAWLLYDWIKHGRPTALGTVSGAVAGLVG
                     ITPAAGFVSPAAAIALGAVTSLGCVTFVTWRTRRGIDDSLDAFGIHGVGGTIGALLTG
                     VFASHNAAGLLTGDARQFGIQIVGVVATYVYVGIVTFILLKVLQGAVGLRVTVEEEMI
                     GLDQTQHGEAGYSM"
BASE COUNT         1394 a         2942 c         2800 g         1489 t
ORIGIN      
        1 accggatcat gcccgacggg gggtcgggat gcgatacgag ttggcggggg ggctcggtcg
       61 gctcaatggt cgcgatgggc gccggggtcg cggccggttc cgtcccggcc gtctcggctg
      121 gcgaaacgac cggttcggcg ggcggctgct cctcgggcgg aatgatcacg atctcctcgt
      181 cggccgccgg cgggccgccc agaccgacgt ccttcaggta aaacacgccc agcacgacga
      241 cgcccagggc aatgacgccc agcacggcgt tgcgcacgcc ggcgggcacc gcgctcgtca
      301 ggcggctcca cagcggccgg cgcgccggcc cggcggcccg cccctcgatg atctcggcga
      361 tgtcgtcctg cgtgaacgac tgcgtcagtt ccagagccga gagcccgtcc tcggtcgaat
      421 aggccgacag cgtgtagtcg gagataatct cgtaagcctt gacgtcgtcg aggccgtatg
      481 cgcgcgcctt catcaccagc agccgcgcct gatcgtcgtt gatcgcgcgg ccgccggcca
      541 cgcgttcgac catccgcttg aagcgccggg cctgaaaatc ggcgatcaac ttgccggcgc
      601 gcagcgcgtc caactggtag cgcggcgcgt cgcgcagcag gttgcgcaga tgctcctcgg
      661 tgacctcgcc gctggcctcc atgccggcca gcatcatctg gaagccctcc tcgccctcga
      721 cggcgatgcg gaactgccag ccttcctcct gagccggctg cgcctgccgg tcggcggcgc
      781 ggcgctgttc catctcggcg cgcacgtgcg tcgcgatgcg ctgcgcctcg gccgaggaaa
      841 tgccctcgcg gacggcgaat tcggtcagcc gttggcgcga ttcagcgtcg agctgcgcgt
      901 cggccatcgc ggcgcgcacc aggtcggcga atcggtcctc ggcgctggcc gtctcgcccc
      961 gctcgagtcg cgccagctcc tcgcggtaga gcgcgcaacg gttagggtcc ttgaggattt
     1021 tgacggcgcg atgaagctgg ttcagcagct tgacgacttc ggcgccgaac ttcggcgagt
     1081 tctgccactt gcgcagcgcc ttcgtccgct cgatcaacgc cttgtcgatc agcccggcgt
     1141 cgttgcagtc gcgcgccacg cccagcagcg cgaagtaatc gaggccgccg tcgccctcgc
     1201 cgaagccgac cgcttcgcgc aacgtcgcgc tgacctcgat gcgcaagctg tccctggcca
     1261 ttattcaata ctcatcatct ggagccgttc gcgcgaggct tcaatggctt cttcgctcat
     1321 ccccattgtg cgttcgatca cggtctggac cacctggtcg atcgtcggca gccgggcggt
     1381 aacgctgagc tgcccatcat gactgcaatg aaaactgact tcaatcggcg ttcccttggg
     1441 caggccggac ggcagccccc gcaccaggca catccccagc tcgacgcacg cctcggggtc
     1501 ctcgccctcg ccttccatca cgacgatgcg gatctgctgc tgattttcga ccgacgtccg
     1561 gaaggtcttg acctcgcggc gcgggatgaa cgcgcggcgc gggatgatcg gcgccaccac
     1621 gcgctgaccc tccttgttga gcgccaggat ccccagcgtg tgcgagttga tcagccgttc
     1681 ctccatctgg cggaagcggt ccagatgctc ctcgcggacc tgcggaatcg gcaccggcag
     1741 cccctgatcg agcatccgga gctgcatcat caccgcgtgg atcgcggccc cctgcgcgac
     1801 gcattcgtcg ggattgagca ccagcttcgg ttcggtgccc agcacctcct tgagcatcgc
     1861 gcgcaccgag cgcatccgcg tcgaaccgcc caccagcagg cactcgtcga tctggccgaa
     1921 cgacaggccg gcctcctgca cggtcagttg cagcgtcgtt tcggtctgga gcagcaggtc
     1981 ctcggtcatc gactcgaact gttcgcgcgt gatgtcgtac gattcggtcg tgccgttcag
     2041 gtgcacggcc agtcgggtcg ccggcagctt cgacagccgc agcttggcct gttcggcctg
     2101 cgtgtagagc ccctgcaacg tctcgtaatc ctcgcgcgga tcgatcccgt gacgcgcgag
     2161 gaactgctcg gcgacgtagt cgatcagccg ctcatcccag tccttgccgc ccagttgcac
     2221 gtcgccgtcg gtggccagca tcgtcacggt gtcgccgctg atgcgcatca cggtcacgtc
     2281 gaacgtgccg ccgccgaggt cgtagaccag gacggtcttg tcggcgccga gcgtgtcgaa
     2341 accataggcc agcgccgccg ccgtcggctc gttgatgatg ctcatcacat tgagcccggc
     2401 aatgcggccg gcgtcctcgg tcgacttgcg ctgctgttcc gtgaagaacg cggggaccgt
     2461 gatcaccgcg tgggtgatct tcttctggcc gcccatggcg atctcggcgt cgcgcaccac
     2521 cttgcgcagg atcatcgccg acagttcctc gggcgaccac tcctggccgc tcagcatgaa
     2581 tcgtttgcgg cgccggccga tgtagggctt gatgaactga atcgtgtggg ccgggttgag
     2641 cgtcgtctgc tccttggcga tggagccgac gatgaccgag gagccgtcga tgtacacggc
     2701 cgacggcgtg gttcgctggt tgtcgctgtt gggaatgatc tgcgcctggc cgtattcatc
     2761 gaccaggccg acgacggagt aagtcgtccc caggtcaatc ccgaccgcga tggcgttgtc
     2821 ggcgaagtca ttcattcagg gggctccagc cagcggaatg cagcggaagg agcgagaccg
     2881 gcgcctggcg gcagcaagcc ggcgagcccg cggagacaaa gggtcgtgac accggtcggg
     2941 acgcgcggtc ggccttcgcg cccgcctggc ggcccggcgc ggcccggcag gccgtttcgc
     3001 gttgggtgac cggcgcgacc gggcgccgcg cgaggcccgc gtcaggcgtg ttgagccggc
     3061 cgccgccggg cgcagccggc gcgcaccgat cccacgtgat cgcggaccgg gaggattcac
     3121 cggacggtgc ctgatgggcc agacgccttg catgggcgca cgaatatgaa tccttttgta
     3181 taacgtcctg tgcgattccg gcaaccgaag ctcgattcaa cgtcccgctc aagcctgtca
     3241 tcaagctgaa ttatggagga agtctggatt gttcgcaagt taagtatcgg cagagtttgg
     3301 cgaactgaag cggggcgctg ggggggatcc cgatcccgat cccgatatcg ttcagattcc
     3361 aatcgcgatc gccggtcaca aacccgcttt gaacaggcga gcggccccgt gcttgtgccc
     3421 gatgaaggtt taattcggtt gacccatcga gcggggatcg gcgataatca aaatacgaca
     3481 tattgctcct atttaccgcg ccccctgtcc cccgcacccg ctccggtccg cccgcttttc
     3541 ccccgctccc cgccctcgct cctcctgccc cgcccccgtt cctccgcgcc ggccgccgcc
     3601 tgcaccacaa cgacgaggtt tgcccccatg accgctcgca gtctcgaaca gatcatccag
     3661 cgccagatcc accgctggaa ttacatcgcc caattgctca attaccatcc gggccgaccg
     3721 ccgggcgacg tgcccgcccc gcgcgaggaa ggcggcacgc gccatccggt cctctgcatc
     3781 tcgcgcgacc tgggggccgg cgcgcgctgg atcgccaagg ccctttgcga acgcctcggt
     3841 taccaactga tgggcagcca actcatcgac gaagtcgccc gcgacctgaa ggtgcagcgc
     3901 agtctgatcg aaacgctcga tgaaaccacg cgcggcgcca tcgagctgat gctcgaaagc
     3961 caggtccggg ggcgccagat cgaaccgggt gattatctgc gcgcgctcta ccgcgtgatt
     4021 caaaccatcg cgatcaaagg gggcgtggtc cttctcggcc gcggcgcccg gttcatcgta
     4081 cgcgaccggt ccgcgctgaa cgtgctgatc accgcctcgc tggagacgcg ctgccggcgc
     4141 tacgccgagc aggagacgtg cggcatccgc gaagccgagg cgcggctgct gcgcgccgat
     4201 cgcgaccgcg ccgagtttat ccgccgttac ttcgggcagg acgtgcgcga cccggcgcat
     4261 tacgatctat cgctcaacac cgaccgcgtc agcgccgagg ccgcggtcga gctgatcctg
     4321 cgcgccctcg agctgcgcgg ttacgacctg tcggtgatgg cgatccgcga cgaccggacc
     4381 ctgcgcgcgg gcggccgcgc cggcgcctcg gcggcggtca agtgatcccg gtgttaagtg
     4441 acgatccgtc atggggcgga tttagcccga tttcgaacct ggtgaggagg cgcgtcatgc
     4501 ccgatccgac gccgcggcgg tccgaagccc tgagccggga aacccgccag gagatcctgc
     4561 tgcgactggc caactgggag cagctcggcg gcgccgtcga ggaccgcgtg acggccgtcg
     4621 ccgacgatcc ggaatgggtg atgttgctgc gctgggagct tggcctggaa ccgttgtcgg
     4681 ccgaggcgcg cctgctgcgc cggcacatcg ccacgctgcg gccgtgcgcc cgccacggcc
     4741 tggcgcggac ccgcgaactg attgaaatga tcggactcaa gaaggcccgc gacctggccg
     4801 actgcggctg caacggcgag gcgcggcact atcacttcgg cgacacgcac cacaacgagc
     4861 tcgtcaagct gctcaagcag gcggcggcct gggccgaggg ggattggatt ggcgcgggcg
     4921 cctgcgggtt gatcctcggc gacgccacgc cggagaaacg ctggctggtc gcctcgctct
     4981 gcaagcagct caagacgcag atccgcagcg ccaatccgcg ccacgcgatt ccgattccgc
     5041 gccaccgctg ttcggcgggc tgaggcggac gtcacgtcgt gccggagcgg gatctcgatc
     5101 ccgaatccga tttcgagatc gattgcgatt tcgataccga tcccgatacc gataggcggc
     5161 ggcgaggcct acgaatcctt cagcggctcg gttttctgct tgcggcggtc gatttccggc
     5221 gccttcagca ggcacgtccc caggaccttg gccccctgct tcagcatcgc gctcaggttg
     5281 cgttgaaatt ccggatgatg ttggccgacg ttctggaagc cctgctcgtt ttcctcgacc
     5341 cgcccccagt tctggaacag gcggccccag tcgctgcgca gcgccagatc catcatcttc
     5401 cggctccggg cgggatacca gtagaggtca tggtacagca cgctggcgac gtaggcccac
     5461 ggcgcgagcc acgtcttgag cgaccactcg atcggccgct tcagcccgcc ccagtagatc
     5521 ttgtgctgca tctggctggc gaaggtcatt ttcttgtacg gcccgacgaa gttccaatcc
     5581 tcgcgggcgg cgtcggcatc gccgacgatt tcgatctcgc gcgggtcgcc gcagcccagc
     5641 ccggcgtcgt gcgccaggcg aatgtagcgg cagtcgacga gcggatcgaa acccatcagc
     5701 cgggcggcga cggcgtcgat cgcgacctga tcggccgacg ccaggatcac gttcttgacg
     5761 tgcggcagca tgcagcgcgg gcccggcccg tcgccggcga acgtcccatc catcaccgcg
     5821 aacacgccgc ggtggatttg cttctggatc atcagcagat ccaccagcgt ttcattgatc
     5881 acagggtgtg tccagtggcg gtgctcgttg agcaggccgc cgaaggcgtt cttcatcgcg
     5941 ccggtcgtcg tcgtgaagac atgcgtcttg accgtcggca gatgaatgat gttttcgccg
     6001 agaaaccgcc ggggaattga aaagcctttg ggatacacct cgttcaggca gaggaacttc
     6061 ttcgtcagat cgcccacggc ctccttgatg ccgatccact cctcgccctc atacagatgg
     6121 atgttgcgca ggccgtattt ctcgacgacg tcgagctgct tgttctcgcg ctcgcccagg
     6181 tgcgaatcga tcacgaccgt ccggttgtgg caggcgtgga tcaggttctt gtcatagccg
     6241 tcggcgagca tcgccgcgat cacgccgtcg agctgccagg gggtggtcga actggcgggg
     6301 aagaagaaat gccacgagat attgaccttc agggccgtgt cggcgtcccg ggcgatcgtc
     6361 tgctgatact cggccagatt gagcagttcg tggtagtcgg accggacggt ggcgggggag
     6421 gtgcgcagga tcgcgacttt ggctctgggc attttttcca ttccctggtt ggacaacggc
     6481 cccgcgagcg acggacgcgc ccgcgcgcgg gctgccttga ttgtcccagc cgtcccccct
     6541 gcgccgcaac gtgaaatccg cccccgggat cgaaaatcct aacttttgac agaccccgtt
     6601 gtcaggaaaa ttctcaattt gggtttgaac cggaaggccg gacgaccggt ataaagcgca
     6661 gggtggaggc cgcaggccgg aaccctgcgt cgccccccta acgcttcaag cccctggaag
     6721 gggcggctgg agctgttgaa tctggggccg ctcagagccg gatttcgacc gcctcctcgg
     6781 tgcagctgag gtagagcggg cagttgatgc aatcggtcca gaccttttgc ggcagcgtgt
     6841 gcttgtcgac aatgcggaaa cccgaacgtt cgaaaaaggg cgggttgtcg gtcagcgcga
     6901 aaacccgatg cagaccgatg cgccgggcct cggccagcag cgcgccgacg agctggacgc
     6961 cggcgccctg accgcggcag tcgggcgcca ccaccagcga acggatctcg gccaggacgg
     7021 gggaaaacag cgccagcgca ccgcaggcga tgatccggcc gtcgcgttcg ccgacgagga
     7081 agtcgcgcag tttttcgcag atgttctcgc gcttgcgcgg cagcacggtc gtcgttcgcg
     7141 acgcctcatc gatcagcccg tggatcgcgt cgatgtcctc cacggtcgcc ggccggaatc
     7201 ggatcgtcgc ccgcggctcg atctggccgg tggcgacggg atcaggttga ggagcttcgc
     7261 tcaatccgga acgcctccgc ttgcaggtgg actctccccg tcggtcgcca gccggccact
     7321 tgccaaccgg caggctgcat tccggtcgat tctatccgga atcccgcacc ggtgccaaga
     7381 tccgattgcc ggcctatcgg cgtcttcatc ggaatcgcta tcgccatcgc tgtcgccatc
     7441 gcgatcgggc tcgatctccc cgcatcccgc agggatgcca gcccgtagcc ggtggttgat
     7501 cgcagcgata ccaccggtca acggccgccc cccaccgcat cccgcaggga tgccagaacg
     7561 acggtgacgc ggccgaacga cgtttctttt tcaatgaatc ccggcgggaa tcccggcggt
     7621 cccaggcagg aaaaattcaa atcgatgggc cggatcgact tggcgcgggc ggaagccccg
     7681 agcgcgccgg ctacagcgcg ttctcgtcgc ggtcgccggt ccggatgcgg atcgcgtcgc
     7741 cgacctcaaa gatgaaaatc ttgccgtcgc cgacgctgcc cgatttcgcc gccgacacga
     7801 tcgcatcagt cgcgtcgtcc acctgattgt cgggcacgac cagatcggcg acgatcttgg
     7861 gacggaacgc gacctggtat tcggcgccgc gatagacctc ggtgtggccg cgctggcggc
     7921 cgtagccccg cgcctcgaag accgtcatgc ccatgatgcc gcgctgcgtc agggcgtcgc
     7981 gcaccgcctc aagccggtga ggctgaataa tggcccggat cattttcatg gatcgaagct
     8041 cccggaatcg gtcaatggtg gacgagcgac gagtgagttc ggcttcaacg cgacgccgcc
     8101 tacatcgaat accccgcttc gccgtgctgc gtctggtcca gcccgatcat ctcttcctcg
     8161 accgtcacgc gcaggccgac cgccccctgc aacaccttga gcagaatgaa cgtcacgatg
     8221 ccgacgtaga catacgtcgc gacaacgccg acgatctgga tgccgaactg ccgcgcgtcg
     8281 ccggtgagca ggccggcggc gttgtgcgag gcgaacacgc cggtcagcag cgcgccgatc
     8341 gtcccgccga ccccgtggat gccgaaggcg tcgagcgaat catcgatgcc gcgccgcgtg
     8401 cgccaggtga cgaacgtgac gcagcccagg ctggtgacgg cgccgagggc gatcgccgcg
     8461 gcgggcgaga cgaacccggc cgccggcgtg atccccacca gcccggccac cgcgcccgag
     8521 accgtgccca gcgccgtcgg ccggccgtgc ttgatccaat cgtagagcag ccaggccagc
     8581 gcccccgcgg cggccgaaac gtgcgtcgtc acgagcgcca gcgcc
//
LOCUS       DAOPCO010000170         8558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7702_length_8558_cov_4.835940, whole genome shotgun sequence.
ACCESSION   DAOPCO010000170 DAOPCO010000000
VERSION     DAOPCO010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7702_length_8558_cov_4.835940"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /locus_tag="PLS90_11625"
     CDS             complement(<1..2068)
                     /locus_tag="PLS90_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03097.1"
                     /translation="MAGNRLCRSWAPVLSLLAAAGACLLAIAPGLDDWLWEGHDHVSA
                     ALRVHMLVRLWQTAGFHYPSWLPEAAVGYGYPFFTFYPPLGYFVAGLVGWVLPISIGL
                     AVRLSFWLSLALAGLAAWVYAWQVLARLAPGPRAAWAFAAAVFYMLAPYHLVDLYLRA
                     ALSQSWIWFTPPLLFAGIELSRRRGGWGAVAVALAAAATLLAHNAIALYVLPLAMLYV
                     ILTAERPAWIWATVAALGGGLALSACYWLPAFGLLPMVRGNSTVVMLPEPYALRNHAV
                     FPIQYFMEGYGRGWSLDGVEDDFATNPGLMLMLCVVLGAARLAAGRVAADLRRQGAVW
                     LGLFLLLAFVMSPLMPWEWMPRTLRYIQFPWRLMVLAIFAAIALVLHTVPPWGGRALP
                     LIAGGLAVWFGVRGLYGELYLPVNPPGGDAALTGMLHEQNVKAEGILGTLGNEYLPNE
                     VDLNYLDPNYLNTHPVPAGRIEPLHPRLQTLLYRHEGRRYEWTTDAPAEARAKLHLFH
                     FPGWRLWIDGRAAPDRLRPAEDGLIEVTVPPGRHRVVLAYDAPPLARPARALSCIALV
                     LALAAAAWLKWFLRRRPFRVVRGLNLAPLRGGRPWGVHLLLLGALLLVGELSLRLLVA
                     TRTGYDLMNLPEEAAPAPHGSFTLPEILFRTAGETGQGSNPSYQLRPGLRGEFLGRML
                     QTDGAGR"
     gene            complement(2514..3248)
                     /locus_tag="PLS90_11630"
     CDS             complement(2514..3248)
                     /locus_tag="PLS90_11630"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPK03098.1"
                     /translation="MGSDHSTRLLVVRIAIAMAVVGLVVALLTVGDGQGRIRRALETI
                     RDLGTAGHAAYFGCVLLVVPLMIPNSWFELGGGILFPVWLASVYVIAAETLGSCLAFV
                     IARFFFSDSLRARLRRRRLFDLTSRVLTHEGWRTVALTRVMPMFPFKLSNYVYGLTDI
                     RLRDFAVGTFAGIIPRCILFVYLGSLIGDLADLGRSGGERSPAEWAVLIAGLLVTLAG
                     MVILANIGRRVVRQKMAEPSPAGPGG"
     gene            3393..4598
                     /locus_tag="PLS90_11635"
     CDS             3393..4598
                     /locus_tag="PLS90_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03099.1"
                     /translation="MTPPRFNRFARWLAFGLAALCLPACSPPTDEEESDEVEWPADLP
                     VAAEVLRTKDRKEDGLTDVAVWRNPQGEPLLFMSASSEDYIAVANPFTGEFIKKIGKE
                     GEKLGRFDRPYAVAPAGNYLFVAERYNQRVQVLELPSYKPVFEFGKGDLDKPYGLEVI
                     EGADGFEVFVADNYSLPDDPNFDRRILQQRIKHYRVDPQADPPSAQLIRAFGEVDNED
                     GQLEDIQFITADATRNRLYICDKEAKDVKVYDLEGNFTGLTFGHDVIERRPGGVEIIE
                     DESAPEGGYLLLVDATRDHTLIHVYSREATQYLGRFTGSPQIERSEGIAVLPTDQPPF
                     TGAALYLIHKERRVHAYSWAEALDALRRAPNPKGTDDSDDDDDDQEGDDAGPAAETVP
                     AAEDAPTTS"
     gene            complement(4632..5549)
                     /gene="moaCB"
                     /locus_tag="PLS90_11640"
     CDS             complement(4632..5549)
                     /gene="moaCB"
                     /locus_tag="PLS90_11640"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019940183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HPK03100.1"
                     /translation="MRDINAKIKTLRMATARATLRVRPETLAAIRERRVPKGDPLEIS
                     KAAAIMAAKNTSQILPYCHPVLVEHVDVQYELGADRIDVEVSVKVIHKTGVEMEALTA
                     AAAAALNLYDMLKMLDDEMEIVGVRLVSKKGGKTDFRNVYARALRAGVLVMSDTVAAG
                     KKEDKSGRLIAERLGELGLEVVRYEIVPDDAETIVARLLAWSDEEQLDLVLTTGGTGL
                     SPRDVTPEAMGRVIGREAPGIAEAARAFGQERTPYAMLSRGRAGLRGGTLIVNLPGSS
                     GGVRESLDALFPYLLHAFKIMRGGGHETK"
     gene            5818..7005
                     /locus_tag="PLS90_11645"
     CDS             5818..7005
                     /locus_tag="PLS90_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HPK03101.1"
                     /translation="MPDNPPRPEELPALSPAELVRYGRHLALPRFGEAGQRRLKRARA
                     LIVGLGGLGCPASLYLAAAGVGTLGLIEFDRVDESNLHRQVLYTSADAGRPKLEVALE
                     RLRAVNPHVRFEPHAARLNADNALDLVSRYDLVLDGADNFATRYLVNDACVLAGRPNV
                     YGSIYRFEGKVALLAAPAGRDHPRGPCYRCLYPEPPPAAAIPNCAEGGVLGVLPGLVG
                     ALQAAEAIKWLARCGEPLAGRVLLIDSLTMEFRTLRVARDPACPVCGDRPTITRVRQQ
                     AARCAASPAAAGPGFSEISPAELHDRMQTADALFLIDVREPFERDICRIDGAHPIPLG
                     QFAHRLDEIPREAEVVIYCKSGGRSAMAAALLARAGYPRVANLTGGILAWADVVDPVM
                     RRY"
     gene            7377..8420
                     /locus_tag="PLS90_11650"
     CDS             7377..8420
                     /locus_tag="PLS90_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPK03102.1"
                     /translation="MASALNRMYSLDIGIDLGTATTLVYVRGEGIIIAEPSVVAIQSG
                     TRRTLAVGADAKRMLGRTPGDIVAIRPMKDGVIADFAVTQSMLAHFITKARVRWRWLP
                     FARIRVVISVPSGITEVEKRAVVESAEQAGADKTFLIDEPMAAAIGSGLPVTEPGGSM
                     IIDIGGGTTEVAVISLGGIVVCISERTAGDKMDEAIVQHIKRKYNLLIGERTAEEIKI
                     RIGSAYELPEERTMEIKGRDLVAGLPRTITITSEEIREALAEPVSAIVNAVKSTLEKT
                     PPELAADLVDKGIIMAGGGSQLKGLDTLLMQETGLNVQLADNPETAIVLGTGKTLDSI
                     RILQNLNGFSHHR"
BASE COUNT         1464 a         3290 c         2555 g         1249 t
ORIGIN      
        1 gccggcccgc cccgtcggtc tggagcatcc ggccgagaaa ctcccctctc agcccgggcc
       61 gaagctgata gctcgggttg gagccttgcc ccgtctcccc ggcggttcga aaaaggatct
      121 ccggcagcgt gaacgaccca tggggcgccg gcgccgcctc ctccggcagg ttcatcagat
      181 cgtatcccgt ccgcgtggcc accagcagcc gcaggctcag ctcgccgacg agcagcagcg
      241 cccccagcag cagcagatgc accccccacg gacgcccgcc gcgcagcgga gccagattca
      301 gacctcggac gacgcggaat ggccggcgcc ggagaaacca cttgagccac gccgcggccg
      361 ccagcgccag caccaacgcg atgcacgaca gtgcgcgcgc cggccgggcc agcggcggcg
      421 catcgtacgc cagcacgacg cggtgccgcc cgggcggcac cgtcacttcg atcagcccgt
      481 cttcggccgg ccggagccgg tcgggcgccg cccgcccgtc gatccacagc cgccagcccg
      541 gaaagtggaa caggtgcaat tttgcccgcg cctcggccgg cgcatcggtc gtccattcgt
      601 agcgccgccc ctcgtggcgg tagagcagcg tctgcagccg cgggtggagc ggctcgatcc
      661 gcccggccgg caccgggtgc gtgttcagat agttcggatc gaggtagttg agatccacct
      721 cgttgggcag gtattcattg cccagcgtgc ccagaatccc ctccgccttg acgttctgct
      781 cgtgcagcat gccggtcagc gccgcgtcgc cccccggcgg attcaccggc agatacaatt
      841 ccccgtatag cccgcggacg ccgaaccaga ccgccaggcc gccggcgatg agcggcagcg
      901 cccggccccc ccacggcggt acggtgtgca gcaccaacgc gatcgccgcg aagatggcca
      961 gcaccatcag ccgccaggga aactggatgt agcgcagcgt ccgcggcatc cactcccacg
     1021 gcatcagcgg cgacatcaca aacgccagca gcagaaacag gccgagccag acggccccct
     1081 ggcgccgcag gtcggcggcg acgcggcccg cggccagccg cgccgccccc agcaccacgc
     1141 agagcatcag catcagcccc gggttcgtcg cgaaatcgtc ctcgacgccg tccaggctcc
     1201 agccccggcc gtagccctcc atgaaatact ggatcgggaa caccgcgtgg ttccgcagcg
     1261 catatggctc gggcagcatc accaccgtgc tgttgccccg gaccatcggc agcaggccaa
     1321 acgccggcag ccagtagcac gccgacagcg ccagcccccc gcccagcgcg gccaccgtcg
     1381 cccagatcca cgccggccgc tcggccgtca ggatcacgta cagcatcgcc agcggcagca
     1441 catacagcgc gatggcgttg tgcgccagca gcgtcgccgc ggcggcgagc gcgaccgcga
     1501 cggcccccca gccccctcgc cggcggctca gttcaatccc cgcaaacagc agcggcggcg
     1561 tgaaccagat ccagctctgc gacagcgcgg cgcgcaggta gagatcgacc agatgatacg
     1621 gcgccagcat gtaaaacacc gccgcggcaa acgcccaggc ggcgcgcggc cccggcgcga
     1681 ggcgcgccag cacctgccag gcgtaaaccc aggccgcaag ccccgccagc gccagcgaca
     1741 accagaaact tagccgaacc gccagcccga tcgagatcgg cagcacccag ccgaccagcc
     1801 ccgccacgaa ataccccagc ggcgggtaga acgtgaagaa cgggtagccg tagccgaccg
     1861 cggcttcggg cagccacgac gggtagtgga accccgccgt ctgccacagg cggaccagca
     1921 tgtgcacgcg caaggccgcg ctgacgtggt cgtgtccctc ccacagccag tcgtccaggc
     1981 ccggggcgat ggccagcagg caggcgccgg ccgccgccag cagcgaaagc acgggcgccc
     2041 aggagcgaca caatcggttt ccggccatcc aggttcctgt cagcgcgacg ttggccggtc
     2101 atttgaaaca ggatgcacgc ggtctgacaa gcttttccca gccggtcggc tcggggaatc
     2161 ttttggggaa tgcggtgcca gagcgttgca gcgcaacgcg tcgactccgc tttgaatccg
     2221 aaacctgcgg ttcacaccaa gggatgaaag tgggggtgaa ccgccgattc gaacagtcca
     2281 gcccttgccg gatccttcag tgcggcaatc ccgtcaggga cggcagactt cagcccgcca
     2341 cgtcagtggc gggaaccggg aaccccatag ccacagagtc ccggagggac gcgaaccgac
     2401 tccaggagat ccggagaata gcggacaaat tttcgagtga gctctgaaag acagacgcac
     2461 tctcgaaccc tgtcaagcag cgtcccctcc tcggcgagat cctctcgcaa cgcttacccc
     2521 cccggccccg ccgggctcgg ttcggccatt ttctggcgca caacccgccg gccgatgttg
     2581 gccaggatga ccatcccggc cagcgtgacc agcagcccgg caatgagcac cgcccattcg
     2641 gcgggcgaac gctccccgcc cgaccgcccc agatccgcca ggtcgccgat cagcgaaccc
     2701 agatacacga acagaatgca gcgcgggatg atcccggcga acgtgcccac ggcgaaatcg
     2761 cgcagccgga tgtcggtcag cccatacaca taattggaaa gcttgaacgg aaacatcggc
     2821 atcacgcgcg tcagcgccac cgttctccac ccctcgtgcg tcagcacccg gctggtgagg
     2881 tcgaacagcc ggcggcggcg caggcgcgcg cgcagcgaat cgctgaaaaa aaagcgcgca
     2941 atcacgaacg cgagacacga gccgagcgtt tcggcggcga tcacgtagac cgacgcgagc
     3001 cagaccggaa aaagaatccc gccccccagc tcaaaccagg aattggggat catcagcggc
     3061 acgaccagca gcacgcaccc gaaatacgcg gcgtggccgg cggtccccag gtcgcggatc
     3121 gtttcgagcg cccggcggat ccggccctgc ccgtcgccca ccgtcagcag cgcgacgacc
     3181 agccccacga ccgccatggc gatggcgatc cggacgacaa gcaggcgtgt ggaatggtcg
     3241 gagcccatgg atgatcctgc cacgacccga cgccggagca caaagcgtac cgccgccgcg
     3301 ccaagccggc ctttaccttt cccccccgac gccctataat cccgtccgat tccgtgcgga
     3361 tggaagccaa ctcctggatt gggaagaaca tcatgacgcc tcctcgcttc aaccgtttcg
     3421 cccgctggct ggccttcggc ctggcggccc tctgcctgcc tgcctgttcc cccccgaccg
     3481 atgaggagga atccgacgag gtcgaatggc cggcggatct gccggtcgcc gccgaggtgc
     3541 tgcgcaccaa ggaccgcaag gaggatggct tgaccgacgt cgccgtctgg cgcaatcccc
     3601 agggcgaacc gctcctgttc atgagcgcca gcagcgagga ctacatcgcc gtcgccaatc
     3661 cgttcacggg tgagttcatc aagaaaatcg gcaaggaagg cgagaaactg ggccggttcg
     3721 accgccccta cgccgtcgcc ccggccggca actacctgtt cgtcgccgaa cgctacaacc
     3781 agcgcgtcca ggtcctcgaa ctgccctcct acaaaccggt ctttgaattc ggcaagggcg
     3841 acctcgacaa accctacgga ctcgaggtaa tcgagggcgc cgatggcttc gaagtcttcg
     3901 tcgccgacaa ctacagcctg cccgacgatc ccaacttcga ccggcgcatc ctccagcagc
     3961 ggatcaagca ctaccgcgtc gacccgcagg ccgacccgcc ctcggcccag ctgatccgcg
     4021 ccttcggcga ggtggacaac gaggacggtc agctcgaaga catccagttc atcaccgccg
     4081 acgccacccg caaccgcctc tacatctgcg acaaggaagc gaaggacgtc aaggtttacg
     4141 acctggaggg caacttcacc gggttgacct tcggccacga cgtgatcgaa cggcgccccg
     4201 gcggcgtcga gatcatcgag gatgagagcg cgcccgaggg cggctacctg ctgctcgtcg
     4261 acgccacgcg cgaccacacc ctgatccacg tctattcgcg cgaggccacg cagtatctcg
     4321 gccgcttcac gggcagtccg cagatcgagc gctccgaggg gatcgccgtg ctgccgaccg
     4381 accagccgcc gttcaccggc gccgccttgt acctcattca caaagaacgg cgcgtccacg
     4441 cctactcctg ggccgaggcg ctcgacgccc tgcgccgggc cccgaacccc aaggggaccg
     4501 atgattcgga cgacgacgat gacgaccagg agggggacga cgcgggcccg gcggctgaga
     4561 ccgtccccgc ggccgaggat gccccgacga cctcctgacc cgccgcgcgg ccggctctgc
     4621 tggagccgcg gttacttcgt ctcgtgcccg cccccgcgca ttatcttgaa cgcgtgcagc
     4681 agatagggaa acagcgcgtc gagcgattcc cgcacgccgc cgctcgagcc cggcagattg
     4741 acgatcagcg tccccccgcg cagtcccgcc cggccccgcg acagcatcgc gtacggcgtc
     4801 cgttcctgtc cgaacgcgcg cgccgcctcg gcgatccccg gcgcctcgcg cccgatcacc
     4861 cgccccatcg cctcgggcgt cacgtcgcgc gggctcagac ccgtcccccc cgtcgtcagc
     4921 accaggtcca gctgctcctc gtcgctccag gccagcagcc gcgccacgat tgtctccgcg
     4981 tcgtcgggaa cgatctcata ccgcacaacc tccaacccca gctcccccag ccgctcggcg
     5041 atcagccggc ccgacttgtc ctccttcttc cccgccgcca ccgtgtccga catgacgagc
     5101 acgccggcgc gcagcgcgcg cgcgtaaaca ttgcgaaaat cggtcttgcc ccccttcttg
     5161 ctcaccagcc gcacgccgac gatctccatc tcgtcgtcga gcatcttgag catgtcgtac
     5221 aggttgagcg ccgccgccgc cgccgccgtc agcgcctcca tctccacgcc cgtcttgtgg
     5281 atcaccttga ccgagacctc gacgtcgatc cggtcggccc ccagttcata ttgcacgtcg
     5341 acatgttcca ccagcaccgg gtggcagtag ggcaggatct ggctcgtgtt cttggcggcc
     5401 atgatcgccg cggccttgct gatttcgagc gggtcgccct tgggcacgcg ccgttcgcgg
     5461 atcgccgcca gcgtctcggg ccgcacgcgc agcgtcgccc gcgccgtcgc catccgcagc
     5521 gtcttgatct tcgcattgat gtctcgcatc ccgtattttc tcccaagaat caaatggccg
     5581 atgatccccc cgtcggcggc tctcatccta tcacacgccc ctccctccag aaccgtccct
     5641 cgtccctcat cctgtcctcg gccccaaggg cctccccaag aacccggctc tcggctctcg
     5701 tccctcgtcc gccattccaa tgtcagcccc acaccctgcc gctttcccgc cggcgccatt
     5761 cccgttcccc cccggcgccg ttcccgctac aattcacccg gaacccggag gcccctcatg
     5821 cccgacaacc caccgcgccc cgaagaactc cccgcccttt cgcccgccga actcgtccgc
     5881 tatgggcgtc acctggccct cccccgcttt ggcgaggcag gccagcgccg cctcaagcgc
     5941 gcccgcgcgc tgatcgtcgg cctcggcggc ctcggctgcc ccgcttcgct ttacctggcg
     6001 gccgcgggcg tcggcacgct cggcctgatc gagttcgatc gcgtcgatga atccaacctg
     6061 catcgtcagg tgctctacac ctcggccgat gccggccgcc ccaagctgga agttgcgctc
     6121 gaacggctcc gcgccgtcaa tccgcatgtc cgcttcgaac cgcatgccgc gcgcctgaac
     6181 gccgataacg cgctggatct cgtctcgcgc tacgacctgg tcctcgacgg cgccgacaac
     6241 ttcgccacgc gctacctggt caacgatgcc tgtgtgctcg ccggccgccc gaatgtttac
     6301 ggcagcatct accggttcga gggcaaggtc gcgctgctgg cggccccggc cgggcgcgat
     6361 cacccgcgcg gcccgtgtta ccgctgcctc tatcccgagc cgccccccgc cgcggccatc
     6421 cccaactgcg ccgagggggg cgtgctcggc gtgctgccgg ggctcgtcgg cgcgcttcag
     6481 gcggccgagg ccatcaagtg gcttgcccgc tgcggcgaac cgctggccgg gcgcgtcctc
     6541 ctgatcgatt cgctgacgat ggagttccgg acgctgcgcg tcgcgcgcga ccccgcctgc
     6601 ccggtctgcg gcgaccggcc gacgattacc cgcgtgcgcc agcaggcggc ccgctgcgcc
     6661 gcttcgccgg cggcggcggg acccggcttc agcgaaatca gccccgccga actgcacgac
     6721 cgcatgcaga ccgccgacgc gctcttcctg atcgatgtgc gcgagccgtt cgaacgggat
     6781 atctgccgca tcgacggcgc gcatccgatc ccgctcggcc agttcgccca tcggctggac
     6841 gagatccccc gcgaggcgga ggtcgtcatt tactgcaaat ccggcggccg cagcgcgatg
     6901 gccgcggccc tgctcgcccg cgccggatac ccccgcgtcg ccaatctgac cggcgggatc
     6961 ctcgcgtggg ccgacgtcgt cgaccccgtc atgcgccgct attaaccccc gccccgcgcc
     7021 ctgaccggga atcgggccgc cctctttctc acgtcccttc cgtcccgttc gtcctttatg
     7081 tccctatagg tcccttccaa aacacgctcg ccgtaccatc cacgccccgc ccgcgccacc
     7141 ctcggaagag gcggttgaca ccaccgattc cccgtgtcag gatacggtcg ggattccggg
     7201 gggcacgcgg tggccctgtg gaatgcattg gattgcggtc tctcctgcgg tggcgggcgc
     7261 gagacgtccc ctgagcgcta cctgttcacc ccgtcaatca ggcacgacac gccccgttga
     7321 tcctgtgatt ggttggccgc gctcagcccg acccggttgc gcggcagtaa cattcgatgg
     7381 cctcagcact caaccgcatg tactcgcttg atatcggcat cgacctgggg accgcgacca
     7441 cgctggtcta cgtgcggggc gagggcatca tcatcgccga accctccgtc gtcgcgatcc
     7501 agtcgggcac ccgccgcacc ctcgcggtcg gcgccgacgc caagcgcatg ctcggccgca
     7561 ccccgggcga tatcgtcgcc atccgaccga tgaaggatgg cgtcatcgcc gatttcgccg
     7621 tcacccagag catgctggcc cactttatca ccaaggcccg cgtccgctgg cgctggctcc
     7681 ccttcgcccg catccgcgtc gtcatctcgg tcccctccgg catcaccgaa gtcgaaaaac
     7741 gcgccgtcgt cgaatcggcc gagcaggccg gcgccgacaa gacgttcctc atcgacgaac
     7801 cgatggccgc cgcgatcggc tcgggcctgc ccgtcaccga gcccggcggc tcgatgatca
     7861 tcgacatcgg cggcggcacc accgaggtcg ccgtcatctc cctcggcggc atcgtcgtct
     7921 gcatcagcga gcgcaccgcc ggcgacaaga tggacgaagc catcgtccag cacatcaagc
     7981 gcaaatataa cctgctcatc ggcgagcgca ccgccgagga gatcaagatc cgcatcggtt
     8041 cggcctacga gctgcccgag gagcggacga tggagatcaa ggggcgcgat ctggtcgccg
     8101 gcctgccgcg cacgatcacg atcaccagcg aggagatccg cgaggcgctc gccgagccgg
     8161 tttccgccat cgtcaacgcg gtcaagtcga cgctggagaa aacccctccc gaactggccg
     8221 ccgacctcgt cgataagggg atcatcatgg cgggcggcgg ctcccagctc aaggggctcg
     8281 atacactcct catgcaggag accggtctca acgtccaact ggccgataat ccggaaaccg
     8341 cgatcgtcct ggggacgggc aagacgctcg attcgatccg gatcctccag aatctcaacg
     8401 gcttctcgca tcaccgctga tccggtcttg ttcttgctcc cccttggcat cgccggcccg
     8461 ccccggcgcc gcttccggtc ggctccacca cgctccgccc ctcggagatt ccccgatgcc
     8521 ttccgacctg aaactgctcg tcgatcgcct ccgcgatc
//
LOCUS       DAOPCO010000171         8363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7905_length_8363_cov_5.847617, whole genome shotgun sequence.
ACCESSION   DAOPCO010000171 DAOPCO010000000
VERSION     DAOPCO010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7905_length_8363_cov_5.847617"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="PLS90_11655"
     CDS             complement(<1..803)
                     /locus_tag="PLS90_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPK03103.1"
                     /translation="MESRRLIFLTGATGHTGSRLARRLAEEGWRARCLLHTPGRRAYL
                     PDHPRIEVVEGSLTEPAGWAASAAGAAALIHAAHVGFAPRVVEACRAAGVERVIALSS
                     TRRFTRFPEETARRVIAGEEALEHSGLAWTVLRPTMIYGGDRDNNLERLVRWLRRRRW
                     LPRVGDGRNLVQPIFVADLVEAVLRALARPVETRGRALTLAGPEPLTQRQLIEEVAAA
                     MGIRVRWVPVPYGLAMAAAGALELCLRRPPVTRAQLRRTLEDKAFDIDEA"
     gene            complement(812..1855)
                     /locus_tag="PLS90_11660"
     CDS             complement(812..1855)
                     /locus_tag="PLS90_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03104.1"
                     /translation="MAQAEVQFCYQCGRRIDKTRKVCWYCNAPIQRTIRPDRTCPFCG
                     GVIPPQAVKCIHCKEWLDGRPRPEPAPAQVQKIVFVVDPRLVQAAHEHRLIGGEPVPR
                     EIAAHLSPQTVLGIERNDPRLIDQPGVRLLPAPEDDAEVIDAEARPVGLLEGPEGAAP
                     SRALMRRGAVQPAGGGGANLPARLSETPQQVGRSLAATLGRALGTFARWLIDRARPPK
                     PVDDTVDVQPDSRYRICESCSTEILATDNYCYHCGMQYHMTTADRHKIRKTYPSNAGN
                     FALIIVLLGGLIGAELSGLAPPPWTAEVVCGLAAFTAAAAFFRRRTSLSQSVSLALLG
                     ASALTYLAVRFIV"
     gene            complement(1965..3188)
                     /locus_tag="PLS90_11665"
     CDS             complement(1965..3188)
                     /locus_tag="PLS90_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPK03105.1"
                     /translation="MNPTHRPLHQPETYDLTGGAGTPIPPTRRRAAAREALASAPGEE
                     DSSHADKLVMLLRQALDEKASDVHLRAGSPPRLRIDGELYQVKGFTPSEEVMWRFFKR
                     MLRADQIEIFERTHELDFSHGLEGFCRIRVNLYLERGQFCAAIRLIPERIPTMEDIKL
                     PAACQKLTELSKGLVLVTGPTGSGKSTTLAALIDYINATRRCHILTIEDPIEYYYEDR
                     KALVTQRELDLDTLSFGAALRHSFRQDPDVVLLGEMRDAETMQIAITLAETGHLTFST
                     LHTGDCTQTLTRIIDAFPPHQQDQIRSQLALSLVAVISQRLLKVKNKRGRIAAREIMI
                     CNRAIRNLIREGKYNQLFSAIQTGTEEGMITMDASLNRLVNEGLVDYRTALEVASDPR
                     EFAGRHGGRAVEEPL"
     gene            complement(3581..5203)
                     /locus_tag="PLS90_11670"
     CDS             complement(3581..5203)
                     /locus_tag="PLS90_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPK03106.1"
                     /translation="MPEITAHVALVLHAHLPYVRHPEHEDPLEERWLFEAVVECYLPL
                     LDVLEGWERDGLDYRLTLSLSPPLLAMLADPLLRERTARHLRRRVELAERELTRTRWL
                     PQFAPLARHYHERMARALEAYEGRWGRDLAAAFAGFAGWGRLELITSSATHAFLPLLQ
                     HEPRAVRAQIGIGLDAAREAFGLRPRGIWSPECGYYPGLDQTLVEEGIGYTILDAHAL
                     LQARRAPRRGVFWPVRTPAGLVVFGRDIESSREVWSGHVGYPGDPWYREFYRDIGYDL
                     EYDYILPYIHESGRRMDTGFKYHRVTGRVNLADKQPYEPERAAERVQAHAEDFLEKRR
                     AQAERLRPWLDRPPLITAPYDAELFGHWWYEGPQWLDAVMRRLAQPDGEVRPTTPGAW
                     LAGHVETLEEVSPQYSSWGMGGYAETWLDASNAWLYPRLHRATREMVALAGEPAGRAE
                     ESTRRVLAQMARELLLAQSSDWAFILRTGTVAEYARKRIEGHLEAFEKLCREVKQGAV
                     DPHELARLEQATPIFPRLDVGRFAGGGSEGGR"
     gene            complement(5232..6542)
                     /gene="hemL"
                     /locus_tag="PLS90_11675"
     CDS             complement(5232..6542)
                     /gene="hemL"
                     /locus_tag="PLS90_11675"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPK03107.1"
                     /translation="MSPESPQETARSARMFSRAREVLPGGVNSPVRAFGAVGGTPRFI
                     RSAAGARLIDVDGNELIDYVGSWGPMILGHAHPQVVAAIREAAGRGTSFGCPSELEVE
                     LAELVRDLVPSIEMVRMVNSGTEAGMAVIRLARGATGRPKIIKMDGCYHGHGDSLLAR
                     AGSGVATFGLPDSAGVTPGTAADTLVVPYNDLDAVAAVLAEAAEQVAAILVEPVAGNM
                     GLVPPAPGYLEGLRELADRHEVLLIFDEVMSGFRVALGGAQARYNVLPDLTMLGKVIG
                     GGLPVGAYGGRRDLMDQVAPLGPVYQAGTLSGNPLAMAAGLATLRILREQGELYDRLE
                     ERARRLGAGLAEAARGAGIAVYPTVVGSMFGLFFQAGPVTDYASARRSDTALFKRWFH
                     GMLAEGIYLPPSQFEACFLSAAHTEEDIERTIAAAGRVLAGLRD"
     gene            7067..7840
                     /locus_tag="PLS90_11680"
     CDS             7067..7840
                     /locus_tag="PLS90_11680"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPK03108.1"
                     /translation="MHLYPSIDSTQDEARRRHAAGQPLAGMVFLAEEQTRGRGRSDRA
                     WHSGPGGLYVTAALPLPMRLPPGYHGWLPLLGALACAETLDEIHGLQTRIKWPNDLFR
                     GEAKLAGLLGDCLGDGYLIGMGLNWANEIEPAALAGRFPATCLAQHARAPLEGRLPFL
                     LHWLDRVQLLQRRLIDDLEAAVDHLAARAEAALWGRARRVHFEQTELGPVAGELLGLT
                     RRAAARVRVAPGRVVELHCGHQTPATGDDPLYDHIEVLA"
     gene            7872..8315
                     /gene="mce"
                     /locus_tag="PLS90_11685"
     CDS             7872..8315
                     /gene="mce"
                     /locus_tag="PLS90_11685"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPK03109.1"
                     /translation="MFKQVDHIGIATRDIDASIATLRKLGPLVVGEREVIERYQLQAV
                     MVRTGDVPIELIQPTSSESNIAAFIDKRGEGVHHVAYRVADVAAALKQAEAEGFRLID
                     QAPRPGYGGSQVAFLHPKSVLGMLTELVQREAGKDEPPYAFEHGH"
BASE COUNT         1250 a         3368 c         2470 g         1275 t
ORIGIN      
        1 gcctcgtcga tgtcgaacgc cttgtcctcc agggttcgcc ggagctgcgc gcgcgtcacc
       61 ggcggccggc gcaggcacag ttccaatgcc cccgccgccg ccatcgccag cccgtacggc
      121 accggcaccc accgcacgcg tatccccatt gccgccgcca cttcctcgat cagttgccgc
      181 tgcgtcagcg gctcgggccc ggccagcgtc agcgcccggc cccgcgtctc aaccggccgc
      241 gccagggccc gcagcaccgc ctccaccagg tccgccacaa agatcggctg caccaggttg
      301 cgcccgtccc ccacgcgcgg cagccaccgc cgtcgccgca gccagcgcac gagccgctcc
      361 aggttgttgt cgcgatcgcc cccgtagatc atcgtcggcc gcagcaccgt ccacgccagc
      421 cccgaatgct ccagcgcttc ctcccccgcg atcacgcgcc gcgccgtctc ttccgggaac
      481 cgcgtgaagc gccgcgtcga ggagagcgcg atcacccgct ccacgcccgc cgcccggcac
      541 gcctcgacca cccgcggagc gaaccccacg tgtgcggcgt gaatcagcgc cgccgccccc
      601 gccgccgacg ccgcccaccc cgccggctcg gtcaggctcc cctccaccac ctcgatccgc
      661 gggtgatccg gcagatacgc ccggcgcccc ggcgtgtgca gcaggcagcg cgcgcgccaa
      721 ccttcctccg ccagccgtcg cgccagccgc gaaccggtat gcccggtcgc gccggtcagg
      781 aagatgagcc tgcgcgattc catcagggag gctaaacaat gaagcgaacg gcgagatacg
      841 tcagcgcgct ggcgcccagc agcgcgagcg aaacgctctg gctcagcgac gtgcgccgcc
      901 ggaaaaacgc cgccgccgcg gtgaacgccg ccagcccgca gaccacttcg gccgtccacg
      961 gcggcggcgc caggcccgac agctccgcgc cgatcagtcc ccccagcagc acgatgatca
     1021 gcgcgaaatt ccccgcgttg gacggatacg tcttgcggat cttgtgccgg tccgccgtcg
     1081 tcatgtgata ctgcatgccg cagtggtagc agtaattgtc cgtcgccaga atctccgtgc
     1141 tgcagctctc gcagatccgg tagcggctgt ccggctgcac gtcgaccgtg tcgtcgaccg
     1201 gtttgggcgg gcgcgcccgg tcgatcagcc agcgcgcgaa cgtccccagc gcgcgcccca
     1261 gcgtcgccgc cagcgagcgc ccgacctgct gcggcgtttc actcaaccgg gccggcaggt
     1321 tcgccccgcc gccacccgcc ggctgcaccg cgccgcgccg catcagcgcc cgcgatggcg
     1381 ccgccccctc cggtccctcc agcagaccga ccggccgcgc ctccgcatcg atcacctccg
     1441 catcgtcctc cggcgccggc agcagccgca cgcccggctg gtcgatcagc cgcgggtcgt
     1501 tgcgctcgat ccccagcacc gtctgcgggc tcaggtgcgc ggcgatctcg cgcggcaccg
     1561 gttcgccccc gatcagccgg tgctcgtgcg ccgcctgcac cagccgcgga tcaacgacga
     1621 agacaatctt ctgcacctgg gccggcgccg gctccgggcg cggccggccg tccagccatt
     1681 ccttgcaatg gatgcacttg accgcctgcg gcggaatcac cccgccgcaa aacgggcacg
     1741 tccggtccgg ccggatcgtc cgctggatcg gcgcattgca gtaccagcag accttgcggg
     1801 tcttatcgat ccgccgcccg cactggtagc agaactggac ctccgcctgc gccatggcaa
     1861 tcccttcgtc cggctcgccg aattggccgg aaacgaaatc ccgaccctcc ggacagaagc
     1921 gctaacttac gtccctgcgt cttgggtcca atgagtccat tgcttcacag cggctcctcc
     1981 accgcccggc cgccgtgccg ccccgcgaac tcgcgcggat cgctcgccac ttccagcgcc
     2041 gtccggtagt ccaccagccc ctcgttgacc agccggttca gcgaggcgtc catcgtgatc
     2101 atcccctcct cggtccccgt ctggatcgcc gagaaaagct ggttgtattt cccttcgcgg
     2161 atcaggttgc ggatcgcccg gttgcagatc atgatctcgc gcgccgcgat ccgcccccgc
     2221 ttgttcttca ccttgagcag gcgctgcgag atcaccgcca ccagcgacag cgccagctgc
     2281 gagcggatct gatcctgctg atgcggcggg aaggcgtcga tgatccgcgt cagcgtctgt
     2341 gtgcaatcac ccgtgtgcag cgtcgaaaac gtcaggtgcc ccgtctcggc cagcgtgatc
     2401 gcgatctgca tcgtctcggc gtcgcgcatc tcgcccagca gcaccacgtc gggatcctgc
     2461 cggaaactgt ggcgcaacgc cgcgccgaac gacagcgtgt ccaggtccag ttcgcgctgc
     2521 gtcaccagcg ccttgcggtc ctcatagtaa tactcgatcg ggtcctcgat cgtcaggatg
     2581 tggcagcgcc gcgtcgcgtt gatgtaatcg atcagcgccg ccagcgtcgt cgacttcccc
     2641 gatcccgtcg gccccgtcac cagcaccagc cccttggaca gctccgtcag cttctggcac
     2701 gccgccggca gcttgatgtc ctccatcgtc gggatccgct cggggatcag ccggatcgcc
     2761 gcgcagaact gcccgcgctc cagatacagg ttcacccgga tgcggcagaa cccctccagc
     2821 ccgtgcgaga agtccagttc gtgcgtccgc tcgaaaatct caatctgatc ggcgcgcagc
     2881 atccgcttga aaaagcgcca catcacctct tccgacggcg tgaacccctt gacctgatac
     2941 agttcgccgt cgatccgcag ccgcggcggc gaccccgccc gcagatgaac gtccgaggcc
     3001 ttctcatcca gcgcctggcg cagcagcatc accagcttgt cggcgtgcga cgaatcctct
     3061 tcgcccggcg cggaggccag tgcctcgcgc gccgccgcgc gccgccgcgt cggcggaatc
     3121 ggcgtgccgg cccctcccgt cagatcgtag gtttcgggct ggtgcagggg gcgatgggtc
     3181 gggttcatgg gcagcggcag ggggctcgcg ggacggcccg cttcggcgcg gggagcggcc
     3241 atggcatggc gtggcggacg cgggatcgcc cgccacgatt cttttcatat ttcccgggcg
     3301 aatccaagca aattcaggtt gcgcgcacgg agcgacggaa acccggctct cacccctcgt
     3361 ccgcgactct cgaatcccgg cgaatgcgcc ggtgttctga tccacccgga gcgatgtccc
     3421 gtcaactttt tggagtgcgg tgccagagcg ttgccacgca acgcgccggc accgctttgg
     3481 atcgatgcgc accgcggatt cggacggatc cagagcggcg tcgacgatcg ctgcgcgctc
     3541 tctgccgccg cacgccatag cgcgccccga ccgcgcccct tcatcttcct ccctcgcttc
     3601 cacccccggc gaatcgcccc acatccagcc gcggaaagat cggcgtcgcc tgctccaacc
     3661 gcgccagctc gtgcgggtca accgcgccct gcttcacctc ccggcacagc ttctcgaacg
     3721 cttccagatg cccctcgatc cgcttgcgcg cgtattcggc cacggtgccg gtgcgcagga
     3781 tgaacgccca gtccgagctt tgcgccagca acagctcgcg cgccatctgc gccagcacgc
     3841 gccgcgtcga ctcctcggca cgccccgcag gctcgccggc cagcgcgacc atctcgcgcg
     3901 tcgcccggtg cagccgcggg tagagccacg cgttggatgc atccagccac gtctcggcat
     3961 acccgcccat gccccacgaa ctgtattgcg gcgagacttc ctccagcgtc tccacgtgac
     4021 ccgccagcca ggcgccgggt gtcgtcggcc gcacctcgcc gtccggttgc gccagccgcc
     4081 gcatcaccgc atcgagccac tgcggtccct cgtaccacca gtggccgaag agctccgcgt
     4141 cgtagggcgc cgtgatcagc ggcggccggt ccagccatgg ccgcagccgt tcggcctgcg
     4201 cccgccgctt ctccagaaaa tcctccgcat gcgcctggac ccgctcggcc gcccgctccg
     4261 gctcatacgg ctgtttgtcg gccagattca cccgccccgt cacgcgatgg tacttgaaac
     4321 cggtatccat ccgccggccg ctctcgtgga tgtagggcag gatgtagtcg tattccagat
     4381 cgtagccgat gtcgcggtag aactcgcgat accacggatc gccggggtaa ccgacgtgcc
     4441 cgctccagac ctcgcgcgag gactcgatgt cgcgcccgaa aacgaccagg ccggccggcg
     4501 tccgcactgg ccagaacacc ccgcgccgcg gcgcgcgccg tgcctgcagc agcgcgtgcg
     4561 cgtccaggat cgtgtagccg atcccctcct cgaccagcgt ctggtccagc ccgggatagt
     4621 agccgcattc cgggctccag atcccccgcg gcctcaaccc gaacgcctcg cgcgcggcat
     4681 ccagcccgat gccgatctgc gcgcggaccg cccgcggctc gtgctgcagc agcggcagaa
     4741 acgcgtgcgt cgcgctcgag gtgatcagct ccagccggcc ccaccccgca aaccccgcga
     4801 acgccgccgc caggtcgcgg ccccagcgcc cctcatacgc ctccagcgcc cgcgccatcc
     4861 gctcgtgata gtgccgcgcc agcggcgcaa actgcggcag ccagcgcgtg cgcgtcagct
     4921 cgcgctcggc caactccacc cgtcgccgca aatgccgcgc cgtccgctcg cgcagcagcg
     4981 ggtcggccag catcgccagc agcggcggcg agagcgacag cgtcagccgg tagtcgagcc
     5041 cgtcgcgctc ccagccctcc agcacgtcca gcagcggcag atagcactcc accaccgcct
     5101 cgaacagcca ccgttcctcc agcggatcct cgtgctccgg atggcgcaca tacggcagat
     5161 gcgcgtgcag caccaaggcg acgtgggctg tgatctccgg catcggttcc cccgtccggc
     5221 gcccgcatcc gctaatcgcg cagcccggcc agcacgcgcc cggccgccgc gatcgtccgc
     5281 tcgatgtctt cctcggtatg cgccgcgctg aggaagcacg cctcgaactg cgacggcggc
     5341 aggtagatcc cctcggccag catcccgtgg aaccaccgct tgaacagcgc cgtatcggac
     5401 cgccgggccg atgcgtagtc ggtcaccgga cccgcctgga agaacaggcc gaacatcgac
     5461 ccgaccaccg tcggatacac ggcaatcccc gcaccccgcg ccgcctcggc cagccccgcg
     5521 cccagccggc gggcccgctc ttccagccga tcgtacagtt ccccctgctc gcgcaggatg
     5581 cgcagcgtcg ccagcccggc cgccatcgcc agcggattgc ccgacagcgt cccggcctga
     5641 tacaccgggc ccagcggcgc cacctggtcc atcaggtcgc gccgcccgcc ataggccccc
     5701 acgggcaggc cgccgccgat caccttgccc agcatcgtca ggtcgggcag gacattgtac
     5761 cgcgcctgcg cgccccccag cgccacgcgg aagccgctca tcacctcgtc aaagatcagc
     5821 agcacctcgt ggcggtcggc cagctcgcgc agcccctcca gataccccgg cgccggcggc
     5881 accagcccca tgttgcccgc caccggctcg accaggatcg ccgccacctg ctcggccgcc
     5941 tccgccagca ccgccgcgac ggcatccaga tcgttatacg gcacaaccag cgtgtcggcc
     6001 gccgtccccg gcgtcacccc ggcgctgtcg ggcaggccga acgtcgcgac gcccgacccg
     6061 gcccgcgcca gcaggctgtc gccatggcca tgataacagc cgtccatctt gatgattttg
     6121 ggccgccccg tcgccccgcg cgccagccgg atcaccgcca tcccggcttc cgtccccgaa
     6181 ttgaccatcc ggaccatctc gatcgacggc accaggtcgc gcaccagctc ggccagctcg
     6241 acctccagct ccgacgggca gccgaacgac gtcccgcggc cggccgcctc gcgaatcgcc
     6301 gccacgacct gcgggtgcgc atgccccagg atcatcggcc cccacgagcc gacgtaatca
     6361 atcagttcat ttccatcgac atcaatcagt cgcgcgcccg ccgccgaccg gatgaaccgc
     6421 ggcgtccccc ctaccgcgcc gaatgcccgc accgggctgt tcacgccgcc gggcagcacc
     6481 tcgcgcgcgc ggctgaacat acgggccgat cgggccgtct cttgcgggct ctcagggctc
     6541 atcgcgcttc atccttactc aagaatccgt ccctcgtcct cactcaaaaa ttcgtccctc
     6601 gtcctcgtga tcgcttccga aggtctatcc tattttgtcc cttccctcac tcaaaaatac
     6661 gccctcgccc tcgtaatcgc ccccaacggc ctcccccaaa attcggcgct cggctctcgt
     6721 ccctcgtcct cggctctcga aaaccgtcat gcgttctccc tctcgtccct cgtccccgtc
     6781 ccccacctcc ctttcgtccc tcacgtccct tatgtccttt gtgtcccttc ccttttgtgt
     6841 cctgccgcca tataagactc actactctga tacgagcttg actttcccct ccatacccgc
     6901 aaaactaggg ctgcccagtg tacctgggtg gctggtaaga tggacgccgt ccccggccgg
     6961 ccgattccac cccgacgatc ctggatccaa cgcgacccgc tccgtgcagc cctcccccag
     7021 cccattcccc ttccagtccc gccccctgcc cgcaccgtgg cgcgcgttgc atctttatcc
     7081 ctcgatcgat tcaacccagg acgaagcgcg gcgccggcac gccgcggggc agccgctggc
     7141 cggcatggtc ttcctcgccg aggaacagac ccgcggccgc ggccgctcgg atcgcgcctg
     7201 gcattccggc cccggcggcc tttacgtcac cgcggcgctc ccgctcccga tgcgcctgcc
     7261 tcccggctac cacggctggc ttcccctgct tggcgcgctt gcctgcgccg aaacgctcga
     7321 cgagatccac ggcctgcaaa cccgcattaa atggcccaac gatctcttcc gcggcgaggc
     7381 caagctggcc ggcctgctgg gcgactgtct cggcgacggt tacctgatcg gcatgggcct
     7441 caactgggcc aacgagatcg agccggcggc cctcgccggc cggtttccgg ccacatgcct
     7501 cgctcagcac gcgcgcgccc cgctcgaggg ccgcctcccc ttcctgctgc actggctcga
     7561 ccgggtccag ctcctgcagc ggcggttgat cgacgatctg gaggccgcgg tggatcacct
     7621 ggcggcgcgg gccgaggccg ccctctgggg ccgcgcgcgc cgcgtccact tcgaacagac
     7681 cgagttgggc cccgtcgccg gcgagctgct cggcctgacg cgccgcgccg ccgcgcgcgt
     7741 ccgcgtcgcc cccggccggg tcgtcgaact gcattgcggc catcagacgc ccgcgacggg
     7801 cgacgatccg ctctacgatc acatcgaggt cctggcttga tttcaactct ccggcgcgaa
     7861 aggcggtgac catgttcaaa caggtggatc atatcggcat tgcaacgcgc gacatcgacg
     7921 cgtcgatcgc gactttgcgc aaactggggc cgctggtcgt cggcgagcgc gaggtgatcg
     7981 agcgctatca acttcaggcg gtaatggtcc gcaccggcga cgtcccgatc gagctgatcc
     8041 agccgaccag ctccgaatcc aacatcgccg ccttcatcga caagcgcggc gaaggggtgc
     8101 accacgtcgc ctaccgcgtg gccgacgtcg cggccgccct caagcaggcc gaggccgagg
     8161 gcttccggct catcgaccag gcgccgcgcc ccggctacgg cggcagccag gtcgccttcc
     8221 tccaccccaa gtccgtcctc ggcatgctga ccgaactggt gcagcgcgaa gccggcaagg
     8281 atgaaccccc gtacgcgttc gaacacgggc actgatcgcc accgggcgcc gcgggccacg
     8341 cccccgcgac ccgttcagcc ctt
//
LOCUS       DAOPCO010000172         8346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7920_length_8346_cov_5.939935, whole genome shotgun sequence.
ACCESSION   DAOPCO010000172 DAOPCO010000000
VERSION     DAOPCO010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7920_length_8346_cov_5.939935"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1443
                     /gene="eno"
                     /locus_tag="PLS90_11690"
     CDS             157..1443
                     /gene="eno"
                     /locus_tag="PLS90_11690"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPK03110.1"
                     /translation="MTTIIQIEGREILDSRGNPTVEVEVLTEGGALGRACVPSGASTG
                     EHEALELRDGDKQRYLGKGVLKAVENVEKIAAEIIGMDCTEQIAIDRAMLDLDGTETK
                     SNLGANAILGVSLACARAAAEAVGLPLYQYLGGVNAKHLPVPMMNILNGGAHADNTVD
                     FQEFMIMPVGLPTFAEALRAGAEIFHALKSVLKAKGLNTAVGDEGGFAPNLASVTETL
                     DTIMQAIDKAGYQPGEQIRIALDVASSEFFDPDKGKYVFKKSTREERTAEEMIDLYAH
                     LCAEYPIISIEDGCAENDWAGWKLLTERLGGTVQLVGDDLFVTNTKFLKRGIDEHCAN
                     SILVKVNQIGTLTETLDAIEMARRARYTAVVSHRSGETEDSTIADIVVATNAGQIKTG
                     SASRSDRIAKYNQLLRIERELGESAIYAGIANFYNL"
     gene            complement(1522..2613)
                     /locus_tag="PLS90_11695"
     CDS             complement(1522..2613)
                     /locus_tag="PLS90_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HPK03111.1"
                     /translation="MNSGNARAASRLQTLPPAYFALVMATGIVSITSLLHGLDGLARG
                     LFALNLAQYAILWGLLILRFARFRANVVADLGDHLRGPGFFTIVAATGVLSAQCFLVA
                     HWPAVGTGLAFFTMLLWMLFVYAVFALLIIKPRKPAFEKGINGGWLVAIVATQSVALS
                     ATLAGTHLGGTEPMLLIVGLLAWSFGVMFYIWVISLIFYRYMFFGFEPGDLSPPYWIN
                     MGAVAISTLAGCRLIGVAGGHPLLGELQPFIKGIVMILWSTATWWIPMLLILGFWRHV
                     VRAFPFKYDPLYWGMVFPVGMYSACTRLLARELNLPFVSPIAWVFLALGIGVWAIVFY
                     GMLRDIGPVVRGVLRGAALAPSAVEGDKG"
     gene            complement(3000..3665)
                     /locus_tag="PLS90_11700"
     CDS             complement(3000..3665)
                     /locus_tag="PLS90_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03112.1"
                     /translation="MRRMVLAAMWVTAALGASRAGAYPEFQAWVDGQTPRNVNCALCH
                     AHGDGPDGVKPGQIGSLSPEQLAALNEARQAFEPGQQVDSPILNEFGDRMVEKLGRTG
                     IIQLRQRPGDLPQAYGFESDLDGDGIPDAREYIDGTLATNAHHGDPWLLLRHNLKANW
                     WHLVLIAIATLLGLYGINNLLAWFEQAIGGDEEAAEEEAGIMKFEIRNSKLETNSNST
                     ILK"
     gene            complement(3665..4771)
                     /locus_tag="PLS90_11705"
     CDS             complement(3665..4771)
                     /locus_tag="PLS90_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HPK03113.1"
                     /translation="MKTAQLVQTSLEFLTDRFPIEKMNVNSLVRTKEVPVHRMSWAYY
                     LGGLALFLFLVQLVTGLMLMFYYEPTVSDAHASVERITEHIGGGALVRNLHTWSASAM
                     IFAVVVHMLTAFAMKAFAKPREITWVSGVLLLLITFAFGFTGYLLPWHQIAVNATKVG
                     LQSIEEAGAYLPGAMSAWPGALKELIQGEASVGQATLSRFYAIHVMVLPLAFFGVLSL
                     HLLSVQLHGMSQGVDRPTGRTERFFPFFILKDAWLWGVVFLVLFVVALCLPFEAFFSY
                     PLFEAYDAMGSTPDGIKPEWYFYFIYYPLELLPFWVIMLAQNAAIAVLLAAPWIFRKT
                     SRRALGVITVAVATYLVVMTVFGQQIYEMFKGGA"
     gene            complement(4768..6093)
                     /gene="narI"
                     /locus_tag="PLS90_11710"
     CDS             complement(4768..6093)
                     /gene="narI"
                     /locus_tag="PLS90_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPK03114.1"
                     /translation="MLDAFLFLGLPYLALVSLVAGSIWRYRRNRFSYSALSSQFLERK
                     QLRWGSLPWHVGLLVVFIGHLVPFVAPGLWQSLVANRGFLLAVETAGVAAALLALGGL
                     TVLAVRRVFSGPLQRITTTMDLIIVGLLIVQVILGLGVALHHRWGAQWSGRTLGPYLW
                     SVVTLRPEAEWVAAMPPLVKLHVAGAFAIFALLPFSRLVHAFSLPVEYLWRAPQKVVW
                     NNPRRWRMRPAAVRQVEESRRYFLRGAGALAGAGVLLGAGVTDKLVRYFRGPEMSQEQ
                     EVALLRKKLERLEMTAEERELELERKRQAIIHVAALAELNRTEGKYFIDYQMRPALAF
                     KDADGLPLLISAKCTHLGCTVASQVDPQGRILCPCHISYFDIATGKPNAGSPAKAPLP
                     HIGWVLMDGAGELIARRAPGGSVEGTPTSQQLEGAQVYIAREYAGGSEA"
     gene            complement(6086..6700)
                     /locus_tag="PLS90_11715"
     CDS             complement(6086..6700)
                     /locus_tag="PLS90_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HPK03115.1"
                     /translation="MQGKLRPGIHDDAGEGELTVNEQNEGSMRERLYPALGELFEPPD
                     EGLDARLAEVCRLAARFDLAAAERLHALARELARLDDGGRAELHARTFELNPACILYV
                     SVHLFGEESFKRAQLMTGLDEAYGAAGFARGGELPDHLGVILRFAPHFSDEEWRDLCR
                     LCLVGAAAKMRDALARSGNPYSYLLQSIHGLLELEVAREVAGHA"
     gene            complement(6646..8142)
                     /gene="narH"
                     /locus_tag="PLS90_11720"
     CDS             complement(6646..8142)
                     /gene="narH"
                     /locus_tag="PLS90_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HPK03116.1"
                     /translation="MDVRAQTAMVFHLDKCIGCHTCSVACKNVWTDRPGAEYMWWNNV
                     ETKPGTGYPTLWEDQLNYNGGWKVGDDGELKLRCQSKADAFLKLFYNPNQPGLDDYYE
                     PWTYQYSELMDAAAGEDQPTARPVSQITGEELKIEAGPNWDDDLSGSPIYAENDPNLK
                     ALSDDDRRMLFEVERLSMMYLPRICNHCANPSCVAACPSGALYKRGEDGIVLINQEVC
                     RGWRNCIAACPYKKIYFNWMTGKSEKCILCYPRVETGQAPACFHTCVGRIRYLGVMLY
                     DAERLVEAMKTPDAGLVDAQRSALLDPHDPRVIEAGRRAGIGEKMLHAAQRSPIWKYV
                     MEWKLALPLHPEFRTMPMLFYVPPLLPVMGRMNDGIYGHDLEELFGKIGEARLPLKFL
                     AALFSAGNVDLVEEVMRKLLAVRYAMRAKELDDVSPERVAQVLREANLTARQAEEIYR
                     LTTLCDANARFVMPPIQREEAMDETSCMAEACKGSCGLGFTTTPARGN"
BASE COUNT         1542 a         3150 c         2301 g         1353 t
ORIGIN      
        1 tctcccgtgc cccctctccc atttcccttg ccattcgccc gcgaaatggg gatcttgtgg
       61 tccgtatgcc cagtggccga gcggcgacgg gttttccccc cgccccgccc gccggtttcg
      121 ccaaagcaga ctggatcgag ccaaggagta ccaatgatga cgacgattat ccagattgaa
      181 ggccgtgaga tcctggactc gcgcggcaac cccaccgtcg aggtcgaagt cctgaccgaa
      241 gggggcgccc tcggccgcgc ctgtgtccct tccggcgcct ccaccggcga acacgaggcc
      301 ctcgaactgc gtgacggcga caagcagcgc tacctgggca agggcgtgct caaggccgtc
      361 gaaaacgtcg aaaaaatcgc cgcggaaatc atcggcatgg actgcaccga gcagatcgcg
      421 atcgaccgcg ccatgctcga cctcgacggc accgaaacca aaagcaacct gggcgccaac
      481 gccattctcg gcgtctcgct cgcctgcgcc cgcgccgccg ccgaggccgt cggcctcccc
      541 ctctaccagt acctgggcgg cgtcaacgcc aagcacctcc ccgtcccgat gatgaacatc
      601 ctcaacggcg gcgcccacgc cgacaacacc gtcgatttcc aggaattcat gatcatgccg
      661 gtcggcttgc cgaccttcgc cgaagccctg cgcgccggcg ccgaaatctt ccatgctctc
      721 aagagcgtcc tcaaggccaa gggcctcaac accgccgtcg gcgacgaggg cggcttcgcc
      781 cccaacctcg ccagcgtcac cgaaaccctc gatacgatca tgcaggccat cgacaaggcc
      841 ggctaccagc ccggcgagca gatccgcatt gccctggatg tcgcctcctc ggagttcttc
      901 gaccccgaca agggcaaata cgtcttcaag aagagcaccc gcgaggagcg gaccgccgaa
      961 gagatgatcg acctgtatgc acacctctgc gccgaatacc cgattatcag catcgaggac
     1021 ggctgcgccg agaacgactg ggcgggctgg aagctgctga ccgaacggct gggcggcacg
     1081 gtccagctcg tcggcgacga cctgttcgtc accaacacga agttcctcaa gcgcggcatc
     1141 gacgagcact gcgccaactc gatcctggtc aaggtcaacc agatcggcac gctgaccgaa
     1201 acgctcgacg ccatcgaaat ggccaggcgc gcgcgctaca ccgctgtcgt cagccatcgc
     1261 tcgggcgaga ccgaggactc cacgatcgcc gacatcgtcg tcgcgaccaa cgccggccag
     1321 attaagaccg gctcggcctc gcgctccgac cgcattgcca aatacaacca gctcctgcgc
     1381 atcgagcgcg aactgggcga atcggcgatc tacgccggca ttgcgaattt ctacaacctg
     1441 tagcccgcgc aaccgccccg cgcgctgaga tccttctcca gaaatccgtc ggatccgccc
     1501 gatccgacgg atttcccatt ctcagccctt atcgccctcg acggcgctgg gcgcgagcgc
     1561 cgcgccccgt agcacgcccc ggaccaccgg gccgatgtcg cgcagcatcc cgtagaacac
     1621 aatcgcccac acgccgatgc ccagcgccag aaacacccac gcgatcggcg agacaaacgg
     1681 caggttcagc tcgcgcgcca gcaaccgcgt gcacgccgaa tacatcccca ccgggaagac
     1741 catcccccag taaagcggat cgtacttgaa cgggaacgcg cgcacgacat gccgccagaa
     1801 gcccaggatc agcagcatcg gaatccacca cgtcgcggtc gaccacagga tcatcacaat
     1861 ccccttgatg aacggctgca attcgcccag tagcggatgg ccccccgcca cgccgatcag
     1921 ccggcagccg gccagcgtcg agatcgccac cgcccccatg ttgatccagt aggggggcga
     1981 caggtcgccc ggctcgaatc cgaagaacat gtagcgataa aaaatcagcg aaatcaccca
     2041 gatgtagaac atcacgccga aactccaggc gagcagcccg acgatcagca gcatcggttc
     2101 cgtcccgccc aggtgcgtcc cggccagcgt ggcgctcagc gcgaccgact gcgtggcgac
     2161 gatcgccacc agccagcccc cgttgatccc tttctcgaac gccggcttcc gcggcttgat
     2221 gatcagcagc gcgaacaccg cgtagacgaa cagcatccac agcagcatcg tgaagaacgc
     2281 cagaccggtc ccgaccgccg gccagtgcgc cacgaggaag cactgtgccg agagcacgcc
     2341 cgtcgccgcc acgattgtga agaaacccgg gccgcgcagg tggtcgccca gatcggcgac
     2401 gacgttggcg cgaaaccgcg cgaaccgcag aatcagcaag ccccacagga tcgcatactg
     2461 cgccaggttg agcgcgaaca gcccccgcgc cagcccgtcg agcccgtgca gcagcgatgt
     2521 gatcgacacg atcccggtcg ccatcaccag cgcaaaatac gccggtggca gcgtctgcag
     2581 gcgcgaagcg gctcgcgcgt tcccgctatt catcggcatt ctcgattccc cgccagcttt
     2641 cgtccctcgt tctcgtcctc ataatcgccc ccagcggcct cccccgaaat tcggctctcg
     2701 gctctcgtcc ctcgtcctcg gctctcgaaa acctgctatg ctgtccccgc gttgtgcttc
     2761 atgaacacct ggaacgaaga cttcgtcctc ctcccggcca tcgctgactc acatgcgtca
     2821 aaagccgtcc gcttgagccg ccccagcggg gcggcatttg aatagctccg ggtggagcgc
     2881 agcggaacct ggagacgccg acacggaaag cccctgtccc cgaagggagc gaacccctgc
     2941 ccatgaaaga gcggatctgt tttcgtcctt tcagggcgac gcccaggacg tcatggttct
     3001 tatttcaata ttgttgaatt cgaatttgtt tcgagtttcg agtttcgtat ttcgaatttc
     3061 atgatccccg cttcctcctc cgccgcctcc tcgtcgccgc cgatggcctg ctcgaaccac
     3121 gccagcaggt tattgatgcc gtacagcccc agcagcgtcg cgatcgcaat cagcacaaga
     3181 tgccaccagt tcgccttcag gttatgccgc agcaggagcc acggatcccc gtgatgcgcg
     3241 ttcgtcgcca gcgtcccgtc aatgtattcg cgcgcatcgg ggatgccgtc gccgtccagg
     3301 tcgctctcga acccgtaggc ctggggcaga tcgcctggcc gctggcgcag ttgaatgatc
     3361 cccgtccgcc caagcttctc gaccattcgg tccccgaact cattcagaat cggactgtcg
     3421 acctgctgac ccggctcaaa tgcctgccgt gcctcgttga gcgccgccaa ctgctccgga
     3481 ctcaggctcc cgatctgacc cggcttgacg ccgtccggcc cgtcgccgtg cgcgtggcac
     3541 agcgcgcaat tgacgttgcg cggcgtctgc ccgtcgaccc acgcctggaa ctccggatat
     3601 gccccggcgc gcgacgcgcc cagcgcggcc gtgacccaca tcgcggctag caccatcctt
     3661 cgcatcaggc gcctcccttg aacatttcgt agatctgctg cccgaacacc gtcatcacca
     3721 ccaggtaggt cgcgaccgcc accgtgatca cgcccagcgc gcgccggctc gtcttgcgga
     3781 aaatccacgg cgccgccagc agcaccgcga tcgccgcgtt ctgcgccagc atgatcaccc
     3841 agaacggcaa cagctccagc gggtagtaaa tgaaatagaa ataccattcc ggcttgatcc
     3901 cgtcgggcgt cgagcccatc gcgtcgtaag cctcgaacag cggataggaa aagaacgcct
     3961 cgaacggcag gcacagcgcg acgacgaaca gcacgagaaa caccaccccc cacagccagg
     4021 cgtctttcaa aataaagaac gggaagaacc gctccgtgcg accggtcgga cggtccaccc
     4081 cctggctcat cccgtgaagc tgcacgctga gcaggtgcag gctcaacacg ccgaaaaacg
     4141 cgagcggcag caccatcacg tgaatcgcgt aaaaccggct cagcgtcgcc tggccgacgc
     4201 tcgcttctcc ctgaatcagt tccttcagcg cacccggcca ggcgctcatc gcccccggca
     4261 ggtaggcccc ggcttcctcg atcgactgca acccgacctt cgtcgcattg accgcgatct
     4321 ggtgccacgg cagcaggtac cccgtgaacc cgaacgcgaa cgtgatcagc agcagcagca
     4381 cgccgctcac ccaggtgatc tcgcgcggct tcgcgaaggc cttcatcgcg aacgccgtca
     4441 gcatgtgcac caccacggcg aagatcatcg ccgacgccga ccacgtgtgc aggttgcgca
     4501 ccagcgcccc gccgccgatg tgttcggtga tccgctccac cgatgcgtgc gcgtcgctca
     4561 ccgtcggctc gtaatagaac atcagcatca gcccggtgac gagctgcacc aggaatagaa
     4621 acagcgccag ccccccaagg taatacgccc agctcatccg gtggaccgga acctccttgg
     4681 tccgcaccag cgagttgacg ttcatcttct cgatcgggaa ccgatcggtc agaaattcca
     4741 gactcgtctg gaccaactgc gcggtcttca tgcctcgctc cccccggcat actcccgggc
     4801 gatgtagact tgcgcccctt ccagttgctg gctggtcggg gtcccctcga cggaaccgcc
     4861 cggcgcgcgc cgcgcgatca gctcgcccgc gccgtccatc agcacccagc cgatgtgcgg
     4921 cagcggcgcc ttggccggcg atcccgcgtt gggcttcccg gtcgcaatgt cgaagtacga
     4981 aatgtggcac ggacacagga tccgcccttg cggatcgacc tggctggcga cggtacaccc
     5041 cagatgcgtg cacttggccg agatcagcag cggcagaccg tcggcgtcct tgaacgccag
     5101 cgccggccgc atctggtagt cgatgaaata tttcccctcg gtccggttca gctcggccag
     5161 cgccgcgaca tgaatgatcg cctggcgctt gcgctccagc tccagctcgc gctcctcggc
     5221 cgtcatctcc agccgctcca gtttctttcg cagcagcgca acctcttgct cctggctcat
     5281 ctccgggccg cggaagtatc gcaccagctt gtcggtcacc cccgccccca gcagcacccc
     5341 ggcgccggcc aacgctcccg ccccgcgcag aaagtagcgc cggctttcct ccacctgccg
     5401 caccgccgcc ggccgcatcc gccaccgccg cggattgttc cataccacct tctgcggagc
     5461 ccgccacaga tactccaccg gcagcgagaa cgcatgcacc agccgcgaga atggcagcag
     5521 cgcgaagatc gcgaacgccc ccgccacgtg cagcttgacc agcggcggca tcgccgccac
     5581 ccattccgcc tccggccgca gcgtcacgac gctccacaga tacggcccca gcgtacggcc
     5641 cgaccactgc gccccccagc ggtggtgcag cgccacgccc agccccagaa tcacctgcac
     5701 gatcagcagc cccacgatga tcaggtccat cgtcgtcgtg atccgctgca acgggcccga
     5761 aaacacccgc cgcaccgcca gcaccgtcag cccgccgagc gccagcagcg ccgccgccac
     5821 ccccgccgtc tccacggcga gtaaaaaccc gcgattcgcg accagcgact gccacaaccc
     5881 cggcgcgaca aacgggacca ggtggccgat gaacacgacc agcagcccga cgtgccacgg
     5941 cagcgacccc cagcgcaact gcttgcgctc gagaaactgc gacgacaatg cgctgtagct
     6001 gaagcggttg cgccggtagc gccagatcga cccggcgacg agcgagacca gcgccagata
     6061 cggcagaccg agaaacagaa acgcgtcaag catgaccggc aacctcgcgg gcgacctcga
     6121 gttccaacaa tccatggatc gattgcagca ggtaggaata cggattgccc gaccgggcga
     6181 gcgcatcgcg catcttcgcc gccgcaccga ccaggcacag ccggcacagg tcgcgccact
     6241 cctcgtcgct gaagtgcggc gcaaagcgca ggatcacccc caaatggtcc ggcagttccc
     6301 ctccgcgcgc gaatccggcc gcgccatacg cttcgtccag gccggtcatc agttgcgcgc
     6361 gcttgaaact ctcctccccg aacaggtgca cgctcacata cagaatgcac gccgggttca
     6421 gctcgaacgt acgcgcgtgc aactcggccc gcccgccgtc gtccagccgc gccagttcgc
     6481 gggccagtgc gtgaagccgt tcggccgcgg ccaggtcaaa ccgcgcggcc agccggcaca
     6541 cttccgccag ccgcgcgtcg agtccctcgt ccggcggctc gaacagctcc cccagcgccg
     6601 ggtagagccg ctcccgcatg cttccctcgt tctgctcgtt caccgtcagt tccccctcgc
     6661 cggcgtcgtc gtgaatccca ggccgcagct tcccttgcac gcttcggcca tgcagctcgt
     6721 ctcgtccatc gcctcctcgc gctggatcgg cggcatcacg aaccgcgcgt tggcgtcgca
     6781 cagcgtggtc agccggtaga tctcctccgc ctgccgcgcc gtcaggttcg cctcgcgcag
     6841 cacctgcgcc acccgttccg ggctcacgtc gtccagttcc ttcgcccgca tcgcgtatcg
     6901 cacggccagc agcttgcgca tcacctcctc gaccaggtcc acgttgccgg cgctgaacag
     6961 cgccgccagg aacttcaacg gcagccgcgc ctcgccgatc ttgccgaaca gctcctccag
     7021 atcgtggccg tagatcccgt cgttcatccg ccccatcacc ggcagcagcg gcggcacgta
     7081 gaacagcatc ggcatcgtcc ggaattccgg gtgcagcggc agcgccagct tccattccat
     7141 cacgtacttc cagatcggcg agcgctgcgc cgcgtgcagc atcttctcgc cgatccccgc
     7201 ccgccgcccc gcctcgatca ctcgcggatc gtgcggatcg agcagcgccg agcgctgcgc
     7261 gtccaccagc cccgcgtccg gcgttttcat cgcttccacc agccgctcgg cgtcatacag
     7321 catcacgccc aggtagcgga tccgccccac gcacgtgtgg aagcacgccg gcgcctggcc
     7381 ggtctccacc cgcggatagc acaggatgca cttctcgctc ttgccggtca tccagttgaa
     7441 atagatcttc ttgtacgggc aggccgcgat gcagttgcgc cagccccggc agacctcctg
     7501 gttgatcagg acgatcccgt cctcgccccg cttgtacagc gcgcccgatg ggcacgccgc
     7561 gacacagctc gggttcgcgc agtgattgca gatccgcggc agatacatca tcgacagccg
     7621 ctcgacctcg aacagcatcc gccggtcgtc gtcgctcaac gccttcaggt tgggatcgtt
     7681 ctctgcgtag atcggcgagc ccgacagatc gtcgtcccag ttcggccccg cttcgatctt
     7741 cagctcctcg ccggtgatct ggctcaccgg ccgcgccgtc ggctggtcct cgcccgccgc
     7801 cgcgtccatc agctcgctgt actgatacgt ccacggttcg taataatcgt ccagccccgg
     7861 ctggttcggg ttgtagaaca gtttcagaaa cgcgtcggcc ttgctctggc agcgcagctt
     7921 caattcgccg tcgtcgccca ctttccaccc gccgttgtag ttgagctggt cttcccacag
     7981 cgtcgggtag ccggtccccg gcttcgtctc gacgttgttc caccacatgt attcggcccc
     8041 cggccgatcg gtccacacgt tcttgcacgc cacgctgcac gtgtggcagc cgatgcattt
     8101 gtcgaggtga aacaccatcg ccgtctgcgc ccgcacgtcc attaaatctc tcccgtctgt
     8161 cccgccagag tctgtttcat ccgtccctcg tctgtcgtcc tcgtgatcgc tcctgaaggc
     8221 ctgcccaatt tcgtcccctc gttgtgaacc cgcggttcat gagctactcg taatcgcccc
     8281 caacggcccg ccccaatatt tctccttacg ttctacctcc gcctcatgat caccgcgtcc
     8341 tcgccc
//
LOCUS       DAOPCO010000173         8344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7924_length_8344_cov_5.357582, whole genome shotgun sequence.
ACCESSION   DAOPCO010000173 DAOPCO010000000
VERSION     DAOPCO010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7924_length_8344_cov_5.357582"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..1175)
                     /locus_tag="PLS90_11725"
     CDS             complement(156..1175)
                     /locus_tag="PLS90_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK03117.1"
                     /translation="MARLTDAAGADGRSRMLRWLGYPYVRALLALVLVFCLGLYFNAD
                     GAFFRWATHRDMLRHSSVYGILACGMTLVIITAGIDLSVGSVLGFIAVAFSILSIHHG
                     WSPWLAIPACLAIGALLGAVSGALIAAFGIQPFIATLAMMVFARGLAKFVSHGMKVSN
                     YVQTPEGYVALDMPAIHGLIDQRILNGNLAVVTLIFLACLLISHVVLARLRWGRYLYA
                     IGGNEEAARLSGVPVARMKLLAYGLAGFFSAVAGICQAAQEYQGDPEAGATYELKAIA
                     IVVIGGTSLMGGRGGILLTLIGTLTIGYMEKILSINAAGEASRLMLTGAIIVAAVLLQ
                     RIRRR"
     gene            complement(1241..2815)
                     /locus_tag="PLS90_11730"
     CDS             complement(1241..2815)
                     /locus_tag="PLS90_11730"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012697183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPK03118.1"
                     /translation="MSAAVPSSPASSPAANPILRMQGIGKSFGGVRALHDVHLELLTG
                     EVHILAGENGAGKSTLIKILAGVHRDYDGRIELDGRPVRFASPQDAAARGIAVIHQEL
                     SLIPSMSVADNIFLGRETVWTGALPWVMRRRQRRRAQQILDQLGLNVDAESTVERLPI
                     SLQQMIEIAKALAAEVRILVMDEPTSALTEPEVERLFALIAELKRRGVAIVYISHKME
                     EIHRIADRLTVLRDGRFVGTAPAAELPPPRLVQWMVGREIGEQFPRHTPHPGPVLLRV
                     EELTLDDPHGLPLPVVDRVGFELRAGEIVGLGGLQGSGASDLLWGLFGARGRPRAGRI
                     ELHGRPYTPRSPGHAIRRGLALLTNDRKTTGLVLGMSITHNITLASLPRLSPGGWLRE
                     RREWAVAARLRERFDIRAADLDLEVQSLSGGNQQKIALAKWLEAGPRVLLLDEPTRGV
                     DVGAKQEIYRLMDAWSAEGYAILLITSEMPELLALSDRILVLHRGRVTACFQRGEATQ
                     ERVLQAAMGGTGEDAA"
     gene            3099..4604
                     /locus_tag="PLS90_11735"
     CDS             3099..4604
                     /locus_tag="PLS90_11735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03119.1"
                     /translation="MALLRPTSSLRRTGLGAGLLLVLLLLIIYGAGWYVVGVARVSQE
                     AELARHLRVLGELALPELSDDALALADLALDARWLAGEDDPASAPLREMSPRARAVYA
                     NYLSERRVAALEGFVARGGLARLLVLAPDTTVLYDTAAPEALLTPFDYAGIDGFELGR
                     AIAGEAAASLAYQRGDQSYKRLYLPVREGLDATSGETRAVLVLVANRDYLHGVEQLAR
                     RLRRHALQLTILTLLIGVLIWRLIRRQRRIESQAAEADRLAALGSLAAGFAHELRNPL
                     GIIRAFAEDLERSTRNAGEPARRAQIEETCHDIVEEIDRMDRLVAQFLEYSRPGAAAT
                     RDRSRTAGEPARLGRDVPAVLGMLRAAAQKRQVELNLEWTPEAEQAGPLPIDSGAFRQ
                     VLLNLVLNALAAAPEQTRVRVQCEVTDGEARLRVTDEGPGIAERDRTRIFDPFYTTRS
                     DGSGLGLTVSRRLVAEAGGSLTLEKRRAQPGASFLVRLPLARPAAAPGVKG"
     gene            4608..6008
                     /locus_tag="PLS90_11740"
     CDS             4608..6008
                     /locus_tag="PLS90_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK03120.1"
                     /translation="MGLRIVLLDDEPKMGKILRRVLEREGHSVRTATEPAAALEAIER
                     EGADLLLTDLKMPGMDGLEVMRRARERVPDLDVIMMTAYATAETAVEAMKQGAADYLI
                     KPFANEELIMVVARVAETRGLRAENRQLKDSLESQAAPRDIVAVSPAMREVMTRVRKV
                     AASNVSVLLRGESGTGKEVLALAIHAGGPRRDKPLVKINCGALPETLLESELFGHTRG
                     AFTGAVETRKGLFEQADGGTIFLDEVGELSPALQVKLLRVLQSGEFQRVGDAQTFRVD
                     VRVIAATHRVLEEMIEEGTFRSDLYYRLNVVPIEIPPLRERPEDVPALIDHFLARIRR
                     RDGQVVNLAPAAFDRLLRYHWPGNIRELENALEHACVMCDGNTIEVRDLPQSVQHGVA
                     PGQAAAETAVPRGGETLEDIERRVLIEALEATGYNHTRAARKLGITRRTLGYRMDKYR
                     IPRRAPNRGEPTQGGA"
     gene            6010..6954
                     /locus_tag="PLS90_11745"
     CDS             6010..6954
                     /locus_tag="PLS90_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03121.1"
                     /translation="MRQQARRWWRIGWVWAALLALALSDGLTTAQQPPPFPPPGQGPP
                     MGGPPGGGGRWRFRGGDPGGFPPPGPGFAREAGPIQTPQELATLLTRLDDLRNRFDQV
                     ERERTRLMDELNARRGDARADHLTPAGLLERQTIERLLADVTARVEEGNHLVRQAAFM
                     TGRLMRQRHRWESWLTPAQPALGGGAQRLTDALAALRAEGEAGFCRALLGDPLGQLAA
                     GALTAVAPPWYRPADELTSPTEAFEEGELPPLPPPGWGQGRNLRPLPGGMRRRLHERL
                     LRMEQRQEEITAMLNQQAVEIERLRLILEQLPETPELE"
     gene            6951..8216
                     /locus_tag="PLS90_11750"
     CDS             6951..8216
                     /locus_tag="PLS90_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03122.1"
                     /translation="MIRPMNSSSHAPGGRTLRRFSGFIRSLPSLAVAAIVLFATVALA
                     QTSPSADPSAWLSLYPPPLQPLAAEAAASPEIADQLHALLAAAHGPEAEALLQPILAL
                     PPPAEDAEGAILAFLEQLLARPPVRALFERALWEALLRLQPVATEASRSLVERIGGIP
                     ATVLWAMPGCREPIPTPVYPYVEVAAALLAQLDFQATLNALRAAPPADRAAMLLQKAR
                     AADPDSAEAAALRAAVHEWDEPARKSLREHLRASAKQASLSPTEQALLVETWLVDRVV
                     PSPDEIEQLDPAAALALLLRSREGEPAFAEAIRRRARQHPATASAAALAAPLPAAPLG
                     AADLRALLEAEGAEGAARIAAAADSAHIAPLEALLLAPETPDALRRRAAMALLLHPDP
                     AARERLRAAADSPRLAAPLRAKLAGWLGQ"
BASE COUNT         1292 a         2784 c         3074 g         1194 t
ORIGIN      
        1 cagacgccgg ccggggcggt tttgttccga ggaattgagg gggcgttttt ttggtggaac
       61 cggaggaaac caagggacat aagggacaga aaaggacata agggacataa aacgatcgcc
      121 cgggtcgaag tggggccggc ttttgttctc gcgtcctacc gccggcggat ccgttgaagc
      181 aggacggcgg cgacgatgat cgcgccggtc agcatcagcc ggctcgcctc gcccgccgca
      241 ttgatactga ggattttttc catgtagccg atcgtgagcg tcccgataag cgtcagcagg
      301 atgccgccgc ggccgcccat caggctggtg ccgccgatga cgacgatcgc gatggccttc
      361 agttcatacg tcgcgccggc ctcggggtcg ccctgatact cctgcgcggc ctggcagatg
      421 ccggcgacgg cggagaaaaa cccggccagg ccgtaggcca gcagtttcat ccgcgccacc
      481 ggcacgcccg acaggcgcgc cgcctcctcg ttgccgccga tggcgtagag gtagcggccc
      541 cagcgcaacc gcgccagcac gacgtgcgag atcagcaggc aggccaggaa gatcagtgtc
      601 acgacggcga ggttgccgtt caggatccgc tgatcgatga ggccgtggat cgccggcatg
      661 tcgagcgcga catagccctc gggcgtctgg acataattgg agactttcat gccgtggctg
      721 acgaatttcg ccaggccgcg ggcgaagacc atcatcgcca gcgtggcgat gaacggctgg
      781 atgccgaagg cggcgatcag cgcgcccgag acggcgccga gcaacgcgcc gatcgccagg
      841 caggccggaa tcgcgagcca ggggctccag ccgtggtgaa tgctgaggat tgaaaaggcg
      901 acggcgatga agcccaggac gctgccgacc gacagatcga tgcccgccgt gataatgacg
      961 agcgtcatcc cgcaggcgag gatgccgtag accgaggaat ggcgcagcat gtcgcgatgg
     1021 gtggcccagc ggaagaaagc gccgtcggcg ttgaaataga gaccgaggca gaagacgagg
     1081 acgagcgcca gcagcgcgcg gacataggga tagcccagcc agcggagcat tcgcgagcgg
     1141 ccgtccgcgc cggccgcgtc agtcaatctt gccatgcctc gattctcccc ccgaaccagt
     1201 acgaaatgga cggccccatc gcaaacgggc ctgcgcgggc tcaggcggca tcctccccgg
     1261 tgccgcccat ggcggcctgc aggacacgct cctgggtggc ttcgccgcgc tgaaaacagg
     1321 ccgtcacgcg cccgcgatgc agcacgagaa tccggtcgct caaggccagc aattcgggca
     1381 tctcggaggt gatgagcagg atcgcatagc cttcggccga ccaggcatcc atcaggcggt
     1441 agatctcctg cttggcgccg acgtcgacgc cgcgggtcgg ctcgtcgagc agcaggacgc
     1501 gcggtcccgc ctccagccat ttcgcgagcg cgatcttctg ctgattgccg ccggagaggg
     1561 actggacctc cagatcgagg tcggcggcgc ggatgtcaaa gcgttcgcgc agccgtgcgg
     1621 cgacggccca ctcgcgccgc tcgcgcagcc agccccccgg cgagagccgc ggcagcgagg
     1681 ccagcgtgat gttgtgcgtg atgctcatcc ccagcaccag cccggttgtt ttgcggtcgt
     1741 tggtcagcag cgcgagcccg cggcggatgg catggcccgg cgagcgcggc gtatagggcc
     1801 ggccatgcag ctcgatccgg ccggcgcgcg ggcggccgcg cgcgccgaac agaccccaga
     1861 gcaggtcgct ggcgcccgaa ccctgcagcc cgccgaggcc gacgatctcg ccggcgcgca
     1921 actcgaagcc gacgcggtcg accaccggca acggcagccc atggggatcg tccagggtca
     1981 gctcctcgac gcggagcagg accggcccgg gatggggggt atggcgggga aactgctcgc
     2041 cgatttcgcg gccgaccatc cactggacaa gccggggcgg gggaagctcg gcggccggag
     2101 ccgtgccgac gaaccggccg tcgcgcagga ccgtcagccg gtcggcgatc cgatggatct
     2161 cctccatctt gtgcgagatg tacacgatcg cgacgccgcg gcgcttcagc tcggcgatca
     2221 gcgcgaacag ccgctcgact tccggctcgg tgagcgcgct ggtcggttcg tccatcacga
     2281 ggatgcggac ttccgccgcc agcgccttgg cgatctcgat catctgctgg agcgagatcg
     2341 gcaggcgctc gacggtcgac tcggcatcga cgttcagccc gagctgatcg aggatttgtt
     2401 gcgcccggcg gcgctgccgg cggcgcatga cccacggcag cgcgccggtc cagaccgttt
     2461 cgcggcccag gaagatgttg tcggcgacgc tcatcgaggg gatcagcgag agttcctgat
     2521 ggatgacggc gatgccccgc gcggcggcgt cctggggcga ggcaaagcgg acggggcggc
     2581 cgtccagctc gatgcggccg tcgtaatcgc gatggacgcc ggcgaggatc ttgatcagcg
     2641 tgcttttgcc ggcgccgttt tcgccggcca gaatgtggac ctcgccggtc aacagctcca
     2701 gatggacgtc gtgcagcgcg cgcaccccgc cgaacgattt gccgatgccc tgcatgcgca
     2761 ggatggggtt tgcggcgggc gatgaggcgg gcgacgatgg gacggcggcg gacatggccc
     2821 ccaacgctat agcgcacggc gcggagggtt gcaagggggt ctccaaattt ctcgtttctc
     2881 gtctctcgtc cctcgtcttc gtgatcgccc caaaggcagg cccctgattt cagcagggac
     2941 atccgggaca caaaaacgga tcgggggcca tcggcgtagg cgatcattgg aacggccgtt
     3001 gcaccttgga gatcagggga gcctttaggc ccgccgggcg tacgcagtgg tacagccttc
     3061 accctttacg gctcaatccc tgtacgatat cgtgcatcgt ggccttgctt cgcccgacct
     3121 cctcgctgcg cagaaccgga ctgggcgccg ggctgctgct ggtcctgctg ctgctgatca
     3181 tctacggcgc cgggtggtac gtcgtcggcg tcgcccgggt ttcccaggag gccgaactgg
     3241 cgcgccacct gcgggtcctc ggcgagctgg ccctgcccga attgagcgac gatgcgctgg
     3301 cgctggccga cctggccctg gatgcgcgct ggctcgccgg cgaggacgac cccgccagcg
     3361 cgccgctgcg ggagatgagc ccccgcgcgc gcgcggtcta cgcgaactat ctgagcgagc
     3421 ggcgcgtggc ggcgctcgaa gggttcgtcg cccgcggcgg cctggcgcgg ctgctggtcc
     3481 ttgcgcccga tacgacggtg ctctacgaca cggccgcccc cgaggcgctg ctgacgccct
     3541 ttgattacgc gggcatcgac ggcttcgaac tggggcgggc gatcgcgggc gaggccgccg
     3601 cttcgctcgc ctatcagcgc ggcgaccaga gctataagcg gctctacctg ccggttcgcg
     3661 aggggctgga cgcgacgagc ggcgagacgc gcgccgtgct ggtgctggtg gccaaccgcg
     3721 attacctgca cggcgtggag cagcttgcgc ggcggctgcg gcgccacgcg ctgcaactga
     3781 cgatcctgac gctgttgatc ggcgtgctga tctggcggct gatccgccgt cagcgacgga
     3841 tcgaaagcca ggcggcggag gccgatcggc tggcggcgct cggctcgctg gcggccgggt
     3901 tcgcccatga gctgcgcaac ccgctgggga tcatccgcgc gttcgcggaa gatctggaac
     3961 gctcgacgcg caacgccggg gaaccggcgc gccgcgcgca gatcgaggaa acctgccacg
     4021 acatcgtgga agagatcgac cggatggatc ggctggtggc gcaatttctg gaatattcgc
     4081 ggccgggggc ggcggcgacg cgcgatcgct cgcgcacggc cggcgagccg gcgcgcctgg
     4141 ggcgcgacgt gccggcggtc ctcgggatgc tgcgcgcggc ggcccagaag cggcaagtcg
     4201 aactgaacct ggagtggacg cccgaggcgg agcaggccgg cccgttgccg atcgattcgg
     4261 gcgccttccg ccaggtgctg ctcaacctgg tgctcaatgc gctggcggcg gcgcccgagc
     4321 agacccgcgt ccgcgtccag tgcgaggtga cggacggcga agcgcggctg cgcgtgacgg
     4381 acgaagggcc cggcatcgcc gagcgcgacc gcacgcgcat ttttgatccg ttctatacga
     4441 cgcgcagcga cgggtcgggg ctgggactga ccgtcagccg gcggctggtg gcggaagccg
     4501 gcggcagcct gacgctcgaa aagcggcgcg cgcaaccggg ggcgagcttt ctggtgcgac
     4561 tgccgctcgc gcgaccggcc gccgcccccg gcgtgaaagg atgaccgatg ggattgcgca
     4621 tcgttctgct ggatgacgaa ccgaagatgg ggaagatcct gcgccgcgtg ctggagcgcg
     4681 aaggccactc ggtgcggacg gcgaccgaac cggccgcggc gcttgaggcg attgaacgcg
     4741 agggggccga cctgctgctg accgacctga agatgccggg gatggacggg ctggaggtga
     4801 tgcgccgcgc gcgtgaacgt gtgcccgatc tcgacgtgat catgatgacg gcctatgcga
     4861 cggcggaaac ggcggtcgag gcgatgaagc agggggcggc ggactacctg atcaagccgt
     4921 tcgccaacga ggaactgatc atggtcgtgg cgcgcgtggc ggagacgcgc ggcctgcgcg
     4981 cggagaatcg ccaactgaag gattcgctgg aatcgcaggc tgccccgcgc gacatcgtgg
     5041 cggtcagccc ggcgatgcgc gaggtgatga cgcgcgtgcg caaggtggcg gccagcaacg
     5101 tctcggtgct gctgcgcggc gaaagcggca ccggcaagga ggtgctggcg ctcgcaatcc
     5161 acgccggcgg accgcggcgc gacaagccgc tggtcaaaat caactgcggc gcgctgcccg
     5221 agacgctgct ggagagcgag ctgttcggcc acacgcgcgg cgcgttcacc ggcgcggtcg
     5281 aaaccagaaa aggtctgttc gaacaggccg acggcggcac gatctttctg gacgaggtcg
     5341 gcgagctctc ccccgcgctg caggtcaagc tgctgcgggt cctgcaatcg ggcgagtttc
     5401 agcgcgtcgg cgacgcccag actttccgcg tcgatgtgcg ggtgatcgcg gcgacgcatc
     5461 gcgtgctgga agagatgatc gaggagggga cgttccgctc cgacctctac taccggctca
     5521 acgtcgtgcc gatcgagatc ccgccgctgc gcgaacggcc cgaggacgtg ccggcgctga
     5581 ttgatcactt tctggcgcgg atccggcgcc gggatgggca ggtggtgaac ctggcgccgg
     5641 cggccttcga ccggctgctg cgctaccact ggccgggcaa catccgtgag ctggagaacg
     5701 cgctggagca cgcctgcgtg atgtgcgacg gcaatacgat cgaggtgcgc gacctgccgc
     5761 aatcggtcca gcacggcgtc gcgccgggcc aggccgcagc cgagacggcg gttccgcgcg
     5821 gcggcgaaac gctggaggac atcgagcggc gggtcctgat cgaggcgctc gaggcgaccg
     5881 gctacaacca tacgcgggcg gcgcgcaagc tcggcatcac gcggcgcacg ctgggctacc
     5941 gcatggacaa gtatcggatc ccgcggcgcg cccccaaccg cggcgagccc acccagggag
     6001 gggcgtgaga tgcgacagca ggcgaggcgt tggtggagaa tcggctgggt gtgggcggcg
     6061 ctgctggcgc tggcgctgag cgacgggctg acgacggcgc agcagccgcc gccgtttccg
     6121 ccgccgggcc agggcccgcc gatggggggt ccgcccggcg gcggggggcg gtggcgcttc
     6181 cggggtggcg atccgggggg ctttccgccg cccggcccgg gcttcgcccg cgaagcgggg
     6241 ccgatccaga ccccgcagga actggcgacg ctgctgacgc gactggacga tttgcgcaac
     6301 cgcttcgacc aggtcgaacg cgagcggacg cggctgatgg atgagctcaa tgcgcgccgg
     6361 ggagacgcgc gggcggatca cctgacgccg gccggcctgc tggagcggca gacgatcgag
     6421 cggctgctgg ccgacgtgac ggcgcgcgtg gaggaaggca accacctggt ccgccaggcg
     6481 gcgttcatga ctggccggct gatgcgccag cggcaccgct gggaatcgtg gctgacgccc
     6541 gcgcagccgg cgctcggcgg cggtgcccag cggctgacgg acgcgctggc ggcgctgcgc
     6601 gcggaaggcg aggcgggatt ctgccgggcg ctgctgggcg atccgctggg gcagctcgcg
     6661 gccggcgcat tgaccgcggt tgcgcccccg tggtaccggc cggcggacga gctgacgtcg
     6721 ccgacggagg cgttcgagga gggcgagctg ccgccgttgc cgccgccagg ctggggtcag
     6781 ggacgcaacc tccggccgct gccgggcggg atgcgacgcc gactgcacga gcgtctgctg
     6841 cggatggagc aacgccagga ggaaatcacg gcgatgctga accagcaggc ggtggaaatc
     6901 gaacggctgc gcctgatcct ggaacaactg cccgaaaccc ccgaactgga atgatccgcc
     6961 ccatgaactc ttcatctcac gcccccgggg gacgaaccct ccggcggttt tctggattca
     7021 tccgctcgct gccgagcctg gccgtcgctg cgatcgtgtt attcgcaacc gtcgccctgg
     7081 cgcagaccag cccctccgcg gatccctcgg cgtggctctc gctctaccca ccaccgctgc
     7141 agccgctcgc ggccgaggca gcggcctcgc cggagatcgc tgaccaactg cacgcgctcc
     7201 tggcggccgc gcacggtccg gaagcggagg cgctgcttca accgattctg gccctgccgc
     7261 cgcccgcgga ggacgcggag ggggcgatcc tggcttttct cgagcagttg ctggcgcggc
     7321 cccccgtgcg cgcgcttttt gaacgggctt tgtgggaagc gctgcttcgc ctgcagccgg
     7381 tcgcaaccga agcctcccgc tccttggtgg aacggatcgg ggggataccg gcgacggtcc
     7441 tctgggcgat gcccggctgc cgcgagccga ttcccacacc ggtttatccc tatgtcgagg
     7501 tcgcggccgc cctgctcgcg caattggact tccaggccac gctgaacgca ctgcgcgcgg
     7561 cgcctcccgc tgatcgcgcg gcgatgctgc tgcaaaaggc gcgagcggcc gatcctgact
     7621 cggcggaggc ggccgccttg cgcgcggctg tgcacgagtg ggatgaaccg gcgcgcaaga
     7681 gtctgcgcga gcacctgcgg gcgagcgcca agcaggcatc gctgtcgccc accgaacagg
     7741 ccctgctcgt cgaaacgtgg ctggtagatc gggtggttcc gtcgccggac gaaatcgagc
     7801 aactcgatcc ggccgcggca ctcgcgttgc tgctgcggtc ccgcgagggc gagccggcat
     7861 tcgccgaagc gatccgccgc cgggcgcggc aacatccggc cacggcctcg gccgccgccc
     7921 tggccgctcc cctgcccgcg gcgccgctcg gggcggccga cctgcgcgcg ctgctggaag
     7981 cggagggtgc ggagggggcg gcccgaatcg ccgcggcggc cgattcggcg cacatcgcac
     8041 cgctggaagc actgctgctc gcgcccgaaa cgccggacgc gctgcgccgc cgcgcggcga
     8101 tggcgctgct gctccacccc gaccccgccg ctcgcgagcg gctgcgcgcg gccgccgact
     8161 cgccgcgact cgcggcgccg ttgcgggcca agcttgccgg gtggcttgga caatgaccgc
     8221 gcgccgcacc cgcttcgccg ccggcgccct gttgtttgtg tgcctggcct ctatggtcaa
     8281 ggcgcagcag gtgacgacga tcaccgcggc cgggctgggc tacccggtgc ccgcgccggt
     8341 ggag
//
LOCUS       DAOPCO010000174         8333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_7939_length_8333_cov_5.660304, whole genome shotgun sequence.
ACCESSION   DAOPCO010000174 DAOPCO010000000
VERSION     DAOPCO010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7939_length_8333_cov_5.660304"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..395)
                     /locus_tag="PLS90_11755"
     CDS             complement(39..395)
                     /locus_tag="PLS90_11755"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HPK03123.1"
                     /translation="MADGREQSLPAACPSKQPEEAGAGGAAAGTDEDQIRRWVQAAQA
                     GDRPSMERLIEHFQDRVWRRARYRIGDYDEAWEVTQDVFVLCFRKIDQYRGEAQFWTW
                     LARIVDNQYRWGSCTP"
     gene            866..4264
                     /locus_tag="PLS90_11760"
     CDS             866..4264
                     /locus_tag="PLS90_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03124.1"
                     /translation="MSSPLGAVEANPPGTPAPSRPELWRYRLDNPAIDTLMVDIDGDG
                     FRDLLTLDQGKLYLREEGGYPLWRERNAELDAVRLTRILTVCNLDAAGAAEIVAESDA
                     DLWLIDAATMRTLARLPETALLGLTDLNEDGAAEIYTAPRQSDDPEFPPVEVSSLADG
                     RLTSLWSHPGCQLMRKPTQDAYQPGESNERFYILDLNDDGAARDLLVALPGGGGWIGL
                     GFNQADQFEQIWQFTDIQDGTSRLRFMDWTDGNYDTYYDVLAYQYDTRRVFNADVVSL
                     GDSIGSFPLRHIDEPRSSAEALKIDRVKPGEWPMRHRDPRWTARSPLKLGMTEAPAEA
                     WNYPLGGSPTGLSLADLDGRDGPDLLVAARGRLSAWSGEGQPLWHSDYLGAPELRGFY
                     DLDGDGRDEMLVYYRHVHSRPTYAALDPATGRELWKFETKPYVASQEMVLTIGKFLPG
                     SKGLQAFSLGPFGFGADLTFFDFSNGLTAAAEPVWRSTVEHVIYPTHDIYDFDSDGDF
                     DFILLEHSRLRIFDLATGRILRDFTWGDYRSYGKMLPIKFENDPVFKLLILSDNSMMN
                     VEMVDINATSGSLMWKVTGKVGFRLTDPAEGIKLKSELERIGDVDGDGRVDVVYLKYN
                     ESGDKRWHGYVRDIVTGEIKQDISLQSDSFEEAGLKAFAQLPEQPARPEPVQATPPPA
                     GSVRVVDPGAGAVEILDAARQRLTALQIPESVSASNWYLQVADLEGDGTPEIIVPRLP
                     DHAAPSTRRPLILTANERIYDTERLILHAPAQSATTAALLRTERGRNLLIFDYDQDGR
                     NDRLDLEPHPEGARLVLTRADGETVWSNVIPSDIEIFQQVTFGEFNGDGYLDVFLNFA
                     ASLINPEVDQRVFTLNGEIVRRCVWDGRTGGLLWHACNVINERPFDYANHPIVYDVNG
                     DGIDDIVSMAPDILAVCSGDSGKLIYDPRYTKSFWRQAGSLYNVPFVEDLDGDGFVEV
                     YGQRAVGGICVMRLPNEPMWVNDLGDQGLYGKVADVEFQGVADVDGDGRMEIGFGLRM
                     GEFRCYAAEDGHLQWSVPVNSPTTEICSADVDGDGRFEFLFGAEDGALYAVRGGDGEA
                     QPVLWKVDLGAPVGPPIVADADGDGQGEILVTTADGFLRCLKAR"
     gene            complement(4325..4984)
                     /locus_tag="PLS90_11765"
     CDS             complement(4325..4984)
                     /locus_tag="PLS90_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03125.1"
                     /translation="MTTPPDPKALKALENEARRCEATDDYLGALDAYEEIQQKGWATA
                     IHIVAMGECYLKNRQRQNAMDLWLKALEEMTDEAPVRQALDRYFPDWERKAALLRKKP
                     ARPAPPRPAERDLPPPPPPPPAFGGDADTPLSVETTRVRQPERAPLRATPPPEAELPA
                     EQTHSLRPGRGGTLSPTPAPAPAAQAGPVQHATAAVNPEYNVNWDFVLQDAAEEMGVK
                     I"
     gene            complement(5027..6451)
                     /locus_tag="PLS90_11770"
     CDS             complement(5027..6451)
                     /locus_tag="PLS90_11770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03126.1"
                     /translation="MFSRQLLGLALVAAGGLTVFLFLWLPFLGAPLAAAWAGLALLLL
                     ANRYPQALLIGLVAVAPFNYGKPFAGASIKLSELIALAMLALILLRLAAGEVERWARL
                     RRAWAPVAAMGVLALLAFATAAPHPNVFNARYELWNYIACAYAILFFRPRHWPQLVGA
                     FVALAGVEAAAALVLYFGFGVTGTSFFSDEAAVRLIRYTAEDIESFAGGMFRLSGTMG
                     HKNLLAAFYVLILPVVVIQMFRRYRPYWLLAIIPGLVVVGLSDSMTGWGAILLIVVLG
                     LLHLRRFDYLALATLLILPLAGAAVYRFGDTIFYRIEQLLSGEEGWGTVSSRFEILEV
                     TLRLLGDYTWSGIGRNNFTAYGETYYGHGHNLYLMKMVEMGVPAGLAFILFILAAIGR
                     AWRPILFEARRLGRQQQYYRALGLWLGCVGFLAMNLFDYNYAHFSLGPLFMMNIGVLL
                     AVAYDFDGERHVGGRDCALSHAAG"
     gene            complement(6723..7538)
                     /locus_tag="PLS90_11775"
     CDS             complement(6723..7538)
                     /locus_tag="PLS90_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPK03127.1"
                     /translation="MDAQFANAAASASAAAAIRWQRLGNVPLSTVTLNEAVEWIARWA
                     GQAPFRLVVTPNVDHLVTLQRDAAFGAAYARADLSLADGQPVVWAARYLGLRGVEKVS
                     GSDLLPALCARGAREGWRAYFVGGRDDEELGLCLRRIGERYPGLRVGGTSPPFGFEAD
                     ARQTDALLGRILEFGPDLLLLGVGSPKSEIWLARHAERLGQGVGLSIGAGMRFVAGLE
                     PRAPRWMQRAGLEWSFRLMREPGRLWRRYLVEDVRFLPLVLRWKREARRRKLN"
     gene            complement(7833..>8333)
                     /locus_tag="PLS90_11780"
     CDS             complement(7833..>8333)
                     /locus_tag="PLS90_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03128.1"
                     /translation="WMIQLPPEAELVDGFAWSEQLAALWRPLPEGSAAGAAILADFTG
                     GAQWAEWSAGGGTLLARKITPNRIDLEVRVSAVDPATGTARLVLHEAAWPGWRARIDG
                     QPALLDRQGPWRALELPSGAREIRLRYEPAGLRQGLLYSAWALVALAVIFARARRRSG
                     RIEWET"
BASE COUNT         1461 a         3030 c         2503 g         1339 t
ORIGIN      
        1 gcggaagtgg atcacgacac ccgcccagcc gatggctttc aaggcgtgca tgagccccag
       61 cggtactggt tgtcgacgat ccgcgccagc cacgtccaga actgcgcctc gccccgatac
      121 tgatcaatct tccggaaaca gaggacaaat acgtcctgtg tcacctccca cgcttcgtca
      181 tagtcgccga tccgatagcg cgcccggcgc cacacccggt cctgaaaatg ctcaatcaac
      241 cgctccatcg acggccggtc gccggcctgc gccgcctgga cccaacgccg gatctgatcc
      301 tcgtcggtcc cggccgccgc gccgccggcc cccgcttctt ccggctgctt gcttggacat
      361 gccgcgggga ggctttgttc ccgaccgtcg gccatcgatt gcctttccca tttggagggg
      421 gatgggggtg aggttggatc ggtcccggcg ggcgcgcccg gcccgctccc gccggcgccg
      481 gcccgggatt ccttccgtta tcacaatatg aacgccccca atgcgcaagg ggggatttgt
      541 ccacgctccc ccacctgtcg cagcccgttc ctccaaaaat cacgtctctc gtccctcgtc
      601 ttcgtccctc gtccctcggc ctcgtctctc gaacgccctc tcccatgtaa ccgacaattc
      661 caatccatat aaccggcaat ttccttgcgg gtaactcagc ttgacattcc acgcccccgg
      721 tgacaggctg gcccatcaat tctcagacga cacgttcccc gcccagcggg gacggcgcga
      781 ttcgaacccc cacggaggcg ccatcgtgga cattacgcgg attttccggg catcccgcct
      841 ttggctggcg gcattcggca tcctcatgag ctcgcccctc ggggccgtcg aggctaatcc
      901 gcccggcacg ccggccccct cccgcccgga gctctggcgc taccgcctgg acaatcccgc
      961 catcgacacc ctcatggtcg acatcgatgg cgatggcttc cgcgatctcc tcacgctcga
     1021 ccagggcaaa ctctatctgc gcgaggaagg gggctaccct ctctggcgcg aacgcaacgc
     1081 cgaactggac gcggtccgcc tgacccgcat cctgaccgtc tgcaatctgg atgcggcggg
     1141 cgcggcggaa attgtggccg aatccgacgc cgacctgtgg ctgatcgacg ctgcgacgat
     1201 gcgcacgctg gcgcggttgc ccgaaaccgc actgctgggc ctgaccgatc tgaacgagga
     1261 cggcgccgcg gaaatctaca ccgcgccgcg ccagagcgac gacccggagt tcccgcccgt
     1321 tgaagtcagc agcctggccg acggccggct gacctcgctc tggtcgcatc ccggctgcca
     1381 actgatgcgc aagccgacgc aggacgccta tcagccgggc gaatccaatg aacgcttcta
     1441 tatcctcgac ctgaacgacg acggcgcggc gcgcgacctg ctcgtggccc tgcccggcgg
     1501 cggcggctgg atcggcctgg gcttcaatca ggccgatcag ttcgagcaga tctggcagtt
     1561 caccgacatc caggacggca ccagccgcct gcggttcatg gactggaccg acggcaacta
     1621 cgacacctat tacgacgtcc tcgcatacca gtacgatacg cgccgcgtct tcaacgccga
     1681 cgtcgtcagc ctgggcgatt caatcggcag cttcccgctg cgccacatcg acgagccgcg
     1741 ttcgagcgcc gaggcgctca agatcgatcg cgtcaaaccg ggcgaatggc cgatgcgcca
     1801 ccgcgatccg cgctggaccg cccgctcgcc gctgaagctc ggcatgaccg aggcgcccgc
     1861 cgaggcttgg aattatccgc tcggggggag cccgaccggc ctctcgctgg ccgaccttga
     1921 tggccgcgac ggacccgatc tgcttgtcgc cgcccgcggc cggctctccg cctggtcggg
     1981 cgaaggccag ccgctctggc acagcgacta cctgggcgcg cccgaactgc gcggctttta
     2041 cgatctcgat ggcgacggcc gcgatgaaat gctggtctac taccgccatg tccattcccg
     2101 gcccacgtat gcggcgctcg accccgccac cgggcgcgag ctgtggaagt tcgaaaccaa
     2161 gccctacgtc gcgtcgcaag agatggtgct gacgatcggc aaattccttc ccggcagcaa
     2221 gggcctgcag gcgttctcgc tgggtccttt cggctttggc gccgatctga ccttcttcga
     2281 cttttccaat gggttgaccg cggccgccga accggtctgg cgcagcaccg tcgagcacgt
     2341 gatctacccc acgcacgaca tctatgattt cgattcggac ggcgatttcg acttcattct
     2401 cctcgagcac agccggctgc ggatctttga tctggccacg ggccggatcc tccgcgattt
     2461 cacatggggc gactaccgca gctacggcaa aatgctgccg atcaaattcg agaacgatcc
     2521 ggtcttcaaa ctgctcatcc tgagcgacaa ttcgatgatg aatgtcgaga tggtcgacat
     2581 caacgctacc tccggttcgc tgatgtggaa ggtcaccggc aaggtcggct tccgcctcac
     2641 cgatccggcc gaggggatca agctgaagtc cgaactggag cgcatcggcg atgtcgacgg
     2701 cgatggccgg gtcgacgtcg tttacctcaa gtacaatgaa tcgggcgata aacgctggca
     2761 cggttatgtc cgcgacatcg tcaccggcga aatcaagcag gatatttcgc tgcaaagcga
     2821 ttcgttcgag gaagccgggc tgaaggcgtt cgcgcaactg cccgagcaac cggcgcgccc
     2881 cgaaccggtc caggccacgc cgccgcccgc cggcagtgtg cgcgtggtgg accccggcgc
     2941 gggcgcagtg gaaatcctcg acgcggcgag gcaacgcctc accgcgcttc agattcccga
     3001 atcggtctcc gcgtcgaact ggtatcttca ggtcgccgac ctggagggcg acggcacgcc
     3061 cgagatcatc gtgccgcggc tgcccgacca cgccgccccc agcacgcgcc ggccgttgat
     3121 cctgaccgcc aacgaacgga tctacgacac ggagcggctg atcctccacg cgccggctca
     3181 atcggcgacg accgccgcgc tgctgcgcac cgaacgcgga cgcaacctgc tgatctttga
     3241 ctacgatcag gacggcagga acgatcgcct cgatctggag ccgcatcccg agggcgcgcg
     3301 cctggtgctg acccgcgccg atggcgagac cgtctggagc aacgtcattc cgagcgacat
     3361 cgagatcttc cagcaggtca ccttcggcga gttcaacggc gacggctacc tggacgtctt
     3421 cctcaacttt gccgccagcc tgatcaaccc ggaagtcgat cagcgtgtct tcaccctcaa
     3481 cggcgagatc gtccgccgct gcgtctggga tggccggacc ggcggtctcc tctggcatgc
     3541 ctgcaacgtc atcaacgagc gccccttcga ctacgccaac catcccatcg tctacgacgt
     3601 caacggcgac gggatcgatg atatcgtcag catggcgccc gacatcctgg ccgtctgctc
     3661 gggcgacagc gggaaactca tctacgaccc acgctacacc aagagcttct ggcggcaggc
     3721 cggctcgctc tataacgtgc cgttcgtcga ggacctcgac ggcgacgggt tcgttgaagt
     3781 ctacggccag cgcgccgtgg gcggcatctg cgtcatgcgg ctgcccaatg agccgatgtg
     3841 ggtcaatgac ctgggggacc aggggctcta tggcaaggta gccgacgtgg aattccaggg
     3901 cgtggccgac gtcgacggcg acggcaggat ggaaatcggc ttcggcctgc gcatgggcga
     3961 attccgctgc tacgcagcgg aggacggcca tctgcagtgg agcgtcccgg tcaattcgcc
     4021 gaccacggaa atctgttcag ccgacgtcga cggggatggc cgctttgagt ttctcttcgg
     4081 cgccgaggat ggcgcgttgt atgccgtgcg cggcggcgac ggcgaagcgc agccggtcct
     4141 gtggaaggtc gatctgggcg ccccggtcgg cccgccgatc gttgccgatg ccgacggcga
     4201 cggccaaggg gaaatcctcg tcaccaccgc cgacggcttc ctccgctgcc tcaaggcgcg
     4261 gtaaggccag tttcaaaaaa acgggaaccg ggatctccgg agtggcgtgg ccccgggcga
     4321 tcgctcagat tttcacgccc atttcctcgg ccgcgtcctg caggacgaaa tcccagttca
     4381 cgttgtattc cggattgacg gcggcggtcg catgctgtac cgggccggcc tgcgcggccg
     4441 gcgcgggcgc gggcgtcggg ctcagcgtcc cgccgcggcc cggacgcagc gagtgagtct
     4501 gctcggccgg gagctcggcc tcgggcggcg gcgtcgcgcg caacggcgcc cgctcgggtt
     4561 ggcgcacccg cgtcgtctcg accgacagtg gcgtgtcggc gtcgccgccg aacgccggag
     4621 gcggcggggg aggcgggggc aggtcgcgct cggccggccg cggcggcgcc ggccgcgccg
     4681 gcttcttccg cagcagcgcc gctttccttt cccagtccgg aaagtaacgg tccagcgcct
     4741 ggcgcaccgg cgcctcatcc gtcatttcct ccagcgcctt cagccacaga tccatcgcat
     4801 tctggcgctg gcggttcttc aggtagcact cccccatggc gacgatgtgg atcgcggtcg
     4861 cccaaccctt ctgctggatc tcctcgtagg catccagcgc gcccaggtaa tcgtcggtcg
     4921 cttcacaccg ccgcgcctcg ttctccagtg ccttgagcgc cttggggtcg ggaggagttg
     4981 tcatctcagc accttccaat ggaggagtcc gctgtcacca gcctagtcaa ccggccgcat
     5041 gcgataaagc gcaatcgcgc ccgccgacgt gccgctcgcc gtcaaaatcg taggccaccg
     5101 ccagcagcac gccgatgttc atcatgaaca gcggccccag actgaaatgc gcgtaattat
     5161 aatcgaacag gttcatcgcc aggaagccga cgcagcccag ccagagcccc agggcgcggt
     5221 agtactgctg ttgccggccc agccggcgcg cctcgaacag gatcggccgc caggcgcgcc
     5281 cgattgccgc cagaatgaac aggatgaacg ccaggcccgc cggcacgccc atctcgacca
     5341 tcttcatcag gtacagattg tggccgtggc catagtaggt ttcgccatag gcggtgaagt
     5401 tgttgcgtcc gatgcccgac cacgtgtagt cgcccagcag ccgcagcgtc acctccagga
     5461 tctcgaatcg cgagctgacc gttccccagc cttcctcgcc cgaaagcaac tgctcgatgc
     5521 ggtagaaaat cgtatcgccg aaccgataga ccgccgcccc cgccagcggc aggatcagca
     5581 gcgtcgccag cgccaggtaa tcgaaccggc gcagatgcag cagccccagc acgacgatca
     5641 gcaggattgc cccccagccc gtcatcgaat cgctcagccc caccaccacc agccccggaa
     5701 tgatcgccag cagccagtat ggccgatagc gccggaacat ctggatgacc accaccggca
     5761 ggatcagcac gtaaaacgcc gcgagcaggt tcttatgccc catcgttccc gacaggcgga
     5821 acatgccgcc ggcgaaactt tcgatgtcct cggcggtata gcggatcaac cgcaccgcgg
     5881 cctcgtcgga gaagaaactg gtgcccgtga ccccgaagcc gaagtagaga accagcgcgg
     5941 cggccgcctc cacgccggcc agcgccacga acgccccgac gagctgcggc cagtgtcggg
     6001 ggcggaaaaa caggatcgca taggcacagg cgatgtagtt ccacagttca tagcgcgcgt
     6061 tgaacacatt ggggtggggc gcggccgtgg cgaacgccag cagcgccagc acccccatcg
     6121 ccgcaaccgg cgcccaggcc cgccgcagcc gcgcccagcg ctcaacctcg cccgcggcca
     6181 gccgcagcag gatcagcgcc agcatcgcga gcgcgatcag ctcgctcagc ttgatcgagg
     6241 cgccggcgaa cggcttgcca tagttgaacg gcgcgaccgc gaccagcccg atcagcagcg
     6301 cctgggggta acggttcgcc agcagcagca gcgccaggcc ggcccacgcc gccgccagcg
     6361 gcgcccccag aaacggcagc cacaaaaaca gaaagacggt cagccccccc gcggcgacca
     6421 gcgccaaccc cagcagttgg cgcgagaaca tcaaggatcg gcgcccgggc gtttccccgg
     6481 ccgatgccgc cccggagctg gatagatcaa cagagggcat tccgcatcaa ttgtccctta
     6541 atctgaatcc gtccctcgca gaaaaattcg gctctcggct ctcgtccctc gtccccggct
     6601 ctcgaaaacc cgctcttcgg atgcctcgta gtgaaccgct ggtttcgcgg tcaccttggt
     6661 cctcggcttc tctttcgtgc ccattcgtgt ctttcgtgat caaaaattct aaatccagaa
     6721 ttctaattca atttccgcct gcgtgcttcc cgtttccagc ggagcaccag cggcaggaag
     6781 cgcacgtcct ccaccagata acgtcgccac agccgcccgg gctcgcgcat caggcggaac
     6841 gaccactcca gccccgcccg ctgcatccag cgcggcgccc gcggctccag cccggcgacg
     6901 aaccgcatcc cggcgccgat gctcaggccg acgccctgcc cgagccgctc ggcgtggcgc
     6961 gccagccaga tttccgattt cggcgacccg actcccagca gcagcaggtc cgggccgaat
     7021 tccagaatcc ggcccagcag ggcatcggtc tgccgtgcgt cagcctcgaa tccgaagggc
     7081 ggcgacgtcc cgccgacccg cagtccgggg taacgctcgc cgatccggcg caggcagagc
     7141 cccagttctt cgtcgtcgcg gccgcccaca aaatatgcac gccagccctc gcgcgccccc
     7201 cgtgcgcaca gcgccggcag cagatccgag cccgaaacct tctccacccc ccgcaaaccc
     7261 agatagcgcg cggcccacac gaccggctgg ccgtcggcca gcgacaggtc ggcccgtgca
     7321 tacgccgcgc cgaacgccgc atcgcgctgc agcgtgacga ggtgatccac attgggcgtc
     7381 acgaccagcc ggaagggcgc ctgtcccgcc cagcgcgcga tccactccac cgcttcgttc
     7441 agggtaacgg tgctcagcgg cacgttgccc agccgctgcc accggatcgc cgctgcggcg
     7501 gacgcggacg cggcggcatt ggcaaactgg gcatccacga cgtgccctcg tcttggaatc
     7561 tcgtccctca cgcaagaatc cgtctctcgt ccctcacgaa aaaatccatc tctcgtcttc
     7621 gcgatcgccc gcaaaggcct tcacaagaat tcggctctcg gctttcgtcc ctcgtcctcg
     7681 actctcgaaa accggtgctt cgcttcattt cgaaccgcgg ttccccccaa cccggcatca
     7741 gtaaacccca acccaacccc ccggtcaaat ccccccgctg ctgccatcga gatcgacatt
     7801 cagtgggctg gcctcctgaa aatcaacacg gttcaggttt cccattcaat tcgacccgaa
     7861 cggcgcctgg cccgcgcgaa gatcacggcc agcgcaacca gcgcccaggc gctgtaaagc
     7921 agcccctggc gcagacccgc cggctcgtag cgcaggcgga tctcccgcgc gcccgacggc
     7981 agctccagcg cgcgccacgg cccttgccgg tcgagcagcg ccggctggcc gtcgatccgg
     8041 gcccgccagc ccggccatgc cgcctcgtgc aggacgagcc gggccgtccc cgtcgccggg
     8101 tcgaccgcgc tgacgcgcac ctccaggtcg atccggttgg gggtgatctt gcgcgccagc
     8161 agtgtgccgc cgccggcgga ccattccgcc cattgcgcgc cgccggtaaa gtcagccagg
     8221 atcgccgcgc ccgccgccga cccttccggc aaggggcgcc acagcgccgc caactgctcg
     8281 ctccaggcaa agccatcgac cagttcggcc tcgggcggca gttggatcat cca
//
LOCUS       DAOPCO010000175         8243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8024_length_8243_cov_4.733512, whole genome shotgun sequence.
ACCESSION   DAOPCO010000175 DAOPCO010000000
VERSION     DAOPCO010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8024_length_8243_cov_4.733512"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLS90_11785"
     CDS             <1..425
                     /locus_tag="PLS90_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03129.1"
                     /translation="PEIPTPEMQYKTVTYAVGTFDGIPNVLIRETRIARSGEEDLVGI
                     APLAFGVLGFDLLFWNPNATPENQYWVTAWNTDERGSFPPPQLPLPASVYIRLTLAAD
                     RRPVESIAPGEPIDTLVLETIVNVEETIGSVLYPRPAT"
     gene            422..3199
                     /locus_tag="PLS90_11790"
     CDS             422..3199
                     /locus_tag="PLS90_11790"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPK03130.1"
                     /translation="MMRITRRSPLPFAPAAGRRGSVILVVVAVLAVLVLLMLTFSYST
                     RVELLAARNWGNTVQARMAAVTGVPQFQDDTLRPISAAWVEPAETGRRRSDLAPVPLQ
                     LRVASLHRYSALEDSPAATEAALGAGSATADNRLAETFLVDTSALLNINAVVPVGEDA
                     ALRVGNRNGAGRGRSAAPPLLSEDVLARLIEDALSAYNIKASVSPRYLAHAIALRRHG
                     PDGRPGVAGRDDNLNSQRAVESRLAAAGANPRRRAGAPAPLEQDGLDNNHDGRVDEVG
                     EGIDEPAEAVSDPRGVAPGDDRPYQSLFDLMTVPGMTPEIFAALAPHLTTLSVSHAAF
                     EAPDLAGEVPYGWPQIDPNTAQPEEIFERLRLRFPQASTPLLAQFAVNVVDRRDADDV
                     PTEFKLDNVSYFGIERNPVINEVCPDTSSFDEDGDDGQFIELFNPYSEPLALEGWRLR
                     GAGPEITLSGALPPGGFLVLTDDFNNATDPTPEDEPGQGSLYNIFGVVPVGESKQIKE
                     FPQLDLDNESGEVKLINPDGQVVDAFAWRNGRWDGAPSSFQRVDPRLRAATRELATPL
                     APNRNQSGATGRSQALEVQETWRNQPYRSALDVMLVSSAWADITQPGQEPEIKAWQLP
                     ALREASDSQLDLRLVDVFRVGARPRGEVEALQRSAAAAHRVPADTAASAHPAALGDLL
                     GASQLADCPVEFGLINVNRASLAVLNALPGMDEQLLLGIRRAREAAWLDPADAARLAA
                     DRLAPIAHDAPEWWTPVSIHHPNRWSTLSEFLLDELVWGNRPLYDRLDAAYPFAGLIT
                     FHSRAIRVGSASRVARAGDQPGDAHRWGQAFAERIITADRGGLETVNFRFLRPIVPGQ
                     VDPDLRYAAPASRAAGLDLRSLINTSDPATLASLARRGLLRPTAKQAVAAPETTGEPQ
                     RTSVAKATAAP"
     gene            complement(3270..4853)
                     /locus_tag="PLS90_11795"
     CDS             complement(3270..4853)
                     /locus_tag="PLS90_11795"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03131.1"
                     /translation="MTWLFYLYVFMVPLQNLQEKLPKGPTGINYANLTMALMVVIWVL
                     ARMTRRRPLLMRSPLNLPLIVLFGLTYLGMMLAYWSGLPEIDSPFAPSSLAVTRILQF
                     FNGFLLFWLAAHLLDTRRKIRQCLIVIALAAPYIFRVFFTQLRSVRSFHFDDDMRVRG
                     PFMFLGSNELGAFFVYSALMLGLLFFLPRRRWEQALFALAASAYAYGILYSYSRATQL
                     AFGLAVILVACLKFRPLIVVLVFAIVTNPLWVPYSVRERWLKTEDEAGRLEQSAQSRK
                     DFWKLATELWEQSPIFGHGVGSFQVLNPRRMDTHNMYLRTLAEQGVVGFAAFMTVWIV
                     ILMMCLRLWRGGPHVFDRYFGFCLMIATVGLMVANIFGDRFTHYVMIGHYWVFVGMAA
                     RLYANMRGDEAMPAEDAYGRPLAGAEGGAAAAVEPAPAGPRLNQFPREPWPARVTWDL
                     PRWGALHLAFRSGASGENEPRVIGPKAELKIRGAARSGPASGSVRDLNVIRPQPEAPA
                     PREDEKPAGLNILRTNSTE"
     gene            complement(5119..6828)
                     /locus_tag="PLS90_11800"
     CDS             complement(5119..6828)
                     /locus_tag="PLS90_11800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03132.1"
                     /translation="MGRRNTPREEPQRAAIPLRPESMPAAGENGSESELPASAPRPPV
                     WRRPIGWLIALAVMLRLVSAPYLISEENPLFDPETFPNGDLIDNMQQARQVLERGGTP
                     EGYFKHGPLYVYLLAGVMKATGGRFWWMYGLQLCAGVAGVALMYLAARRLIGEPGATA
                     AGALAALYAPAVYYETVMTADALNPVVVALILYLGARYQTHPQASAWRIPVLLGLALG
                     GLTMLRANLCLIGLALAVWLPAAPAAWAAGMSRGRVWRRRLGALVLIAGVSLITLIPV
                     SIYNSRVAGRPRFVVGESNIIWQFSWSVDSPGYFYYPEGFPESELLAPTDPRVLALTA
                     RKLRQFFAWLEFPDLTNFHLYSRLSPPLHADPVTYRLVAPLGLVGLVLALFHPRRFSL
                     LLGMFAVVLFAQILFYVSGRYRLTITPLLFLFAGLLAQRAVELIRGRGLARRRLPALA
                     GIAAGLLAAGALVNVYDVSRLHSMWHSDSIFGNFALTRGAQAFAAGELEQARKFANRL
                     MWLDYPEYQMQGHILLSEIARVEQKPYEMRRHLEEAERIRRARAALPVLDAFGNKHFP
                     PST"
     gene            complement(6859..7608)
                     /locus_tag="PLS90_11805"
     CDS             complement(6859..7608)
                     /locus_tag="PLS90_11805"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HPK03133.1"
                     /translation="MPAEFLRGAVDVWRGLRFLLGHPGLWIWALIPFTINVLLFAGVA
                     WAVWHFAGGWLERAFFAQGGFWADLFGYLLGALIWIALGLTTLFAFVPMAALIASPFN
                     DVLSEKVEVIYGGGTVEESFSLKALWRGLRVGLATSLRLFWVTLLLFICTLPLHLAPV
                     IGSAAAAVASAAITIRFLALEFTSYSMDRRYYDYARRREFLRRNRWRTLGLGTMAFVI
                     MPVPLLNALFIPVSAVAGTLLFCDSELRERG"
     gene            complement(7639..7899)
                     /locus_tag="PLS90_11810"
     CDS             complement(7639..7899)
                     /locus_tag="PLS90_11810"
                     /inference="COORDINATES: protein motif:HMM:NF021381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK03134.1"
                     /translation="MRHGCKDQLAREFVCPKCHGHGAQVQEVSVGRAMVNLLPLPANS
                     YLAVTCGLCGYTEFYHLAIAEKVVESASELADPRKLTQKTDP"
BASE COUNT         1458 a         2944 c         2664 g         1177 t
ORIGIN      
        1 accccgaaat ccccaccccc gagatgcagt acaagaccgt gacctacgcc gtcggcacgt
       61 tcgacggcat ccccaacgtc ctgatccgcg agacgcgcat cgcccgctcc ggcgaggagg
      121 acctggtcgg catcgcgccg ctggcgttcg gcgttctcgg cttcgatctg ctcttctgga
      181 atccgaatgc cacaccggag aatcagtatt gggtcaccgc gtggaacacc gacgagcggg
      241 ggagtttccc gccgccgcaa cttccgctgc cggccagtgt ctatatccgg ttgacgctcg
      301 ccgccgatcg gcggccggtc gagtcgatcg ccccgggcga gccgatcgac accctcgtcc
      361 tggaaaccat cgtcaacgtc gaggaaacca tcggcagcgt gctttacccg aggccggcaa
      421 catgatgcgg atcacccgac gctccccgct cccgttcgcc cccgccgccg gccgccgcgg
      481 ctcggtgatc ctcgtcgtcg tcgcggtgct ggccgtgctg gtcctgctga tgctgacgtt
      541 cagctacagc acgcgggtgg agctgctggc cgcgcgcaac tggggcaaca cggtgcaggc
      601 gcgcatggcc gccgtcacgg gcgtcccgca attccaggat gacaccctgc ggccgatcag
      661 cgccgcgtgg gtcgagccgg ccgagaccgg gcggcgtcgc agcgatctgg cgccggtgcc
      721 cctgcagctt cgcgtcgcgt cgctgcaccg ttacagcgcg ctggaggatt cgcccgccgc
      781 gaccgaggcg gcgctcggcg ccggctccgc gacggcggac aaccggctgg cggaaacctt
      841 cctcgtcgat acgtcggctc tgctcaacat caacgcggtc gtgccggtcg gcgaagacgc
      901 ggcgctgcgc gtgggcaacc ggaacggcgc gggccggggc cggtcggccg cgccgccgct
      961 gctgagcgag gatgtcctcg cccgccttat cgaggacgcg ctgtcggcct acaacatcaa
     1021 ggcgtcggtc tcgccgcgtt atctggcgca cgcgatcgcg ctgcgacgcc atgggcccga
     1081 tgggcgtccc ggcgtcgccg gccgtgatga caatctcaat agccagcgcg cggtggaaag
     1141 ccggctggcc gccgccggcg ccaatccacg ccgccgcgcc ggcgcgcccg cgcccctcga
     1201 acaggatggg ctcgacaaca accacgacgg ccgggtcgat gaggtcggcg aggggatcga
     1261 cgaaccggcc gaggccgtca gcgatccgcg gggcgtggcg ccgggcgacg accggcccta
     1321 ccagtccctg ttcgatctga tgacggtccc cgggatgacg ccggagattt tcgccgcgct
     1381 cgccccgcac ctgacaacgc tgtcggtctc gcacgccgcg ttcgaagccc ccgatctggc
     1441 gggcgaggtc ccctacggct ggccgcagat cgatcccaac acggcccagc ccgaagagat
     1501 cttcgagcgg ctccggctgc gcttccccca ggcttccacg cccctgctgg cgcagttcgc
     1561 cgtcaacgtg gttgatcggc gcgacgccga cgatgtcccc accgagttca agctcgacaa
     1621 cgtctcgtac ttcggcatcg agcggaaccc cgtgatcaac gaggtctgcc ccgatacatc
     1681 cagcttcgac gaggatggcg acgacggtca gttcatcgag ctgttcaacc cctattccga
     1741 gccgctcgcc ctggagggct ggcggctccg cggcgccggt cccgaaatca cgctgtcggg
     1801 cgccctgccg cccggcgggt tcctcgtcct gaccgacgac ttcaacaacg ccaccgatcc
     1861 gacgccggag gacgaacccg gtcagggatc gctctacaat atcttcggcg tggtgccggt
     1921 cggcgagtcc aagcagatca aggagttccc ccaactggac ctggataacg aatcgggcga
     1981 ggtcaagctg atcaaccccg acggccaggt tgtggacgcc tttgcgtggc gcaatggccg
     2041 gtgggacggc gcgccgtcgt cgttccaacg ggtcgatccg cgcctgcgcg cggccacccg
     2101 cgaactggcg acgccgctcg cgcccaaccg caaccagtcc ggcgcgacgg gccgcagcca
     2161 ggcgctcgag gttcaggaaa cgtggcgcaa tcagccctac cgcagcgcgc tcgacgtgat
     2221 gctggtcagt tcggcgtggg ccgatatcac gcagcccggc caggaaccgg agatcaaggc
     2281 gtggcagctg ccggcgctgc gcgaggcgag cgattcgcag ctcgacctgc ggctggtcga
     2341 cgtgttccgc gtgggggcgc ggccgcgcgg cgaggtcgag gcgctgcagc gctccgccgc
     2401 cgccgcccac cgggtgccgg ccgacaccgc cgcgtccgct cacccggccg ccctcggcga
     2461 ccttctcggc gcttcccagc tcgccgattg cccggtcgaa ttcgggctga tcaacgtcaa
     2521 ccgcgcgtcg ctcgccgtgc tcaacgcctt gccgggcatg gatgagcaac tgctgctggg
     2581 gatccggcgc gcgcgcgagg cggcctggct cgatccggcc gacgcggcgc gcctcgcggc
     2641 cgaccgtctc gcgccgatcg ctcacgatgc gccggagtgg tggacccccg tctcgatcca
     2701 tcaccccaac cgctggtcca cgctgtcgga gttcctgctc gatgagctcg tctggggcaa
     2761 ccgtccgctc tacgaccggc tggatgcggc ctatccgttc gcggggctga tcacgttcca
     2821 ttcacgggcg atccgcgtcg gcagcgccag ccgggttgcc cgcgccggcg atcagcccgg
     2881 cgacgcgcat cgctggggcc aggccttcgc cgagcgcatc atcaccgccg accgcggcgg
     2941 cctcgagacc gtgaatttcc gcttcctgcg gcccatcgtg ccaggccagg tcgatccgga
     3001 cctgcgctat gcggcgcccg cgagccgggc cgccggattg gatctgcgct cgttgatcaa
     3061 cacatccgat cccgccacgc tggcttcgct cgcccgccgc gggctgctgc gaccaaccgc
     3121 gaagcaagcc gtcgccgcgc cggagacgac cggcgaaccg caacgcacca gcgtcgccaa
     3181 agccaccgcc gcgccgtaag cgggcgccgc cccgggcgaa tccgacgagc ctctctctcc
     3241 gtattgcttc ttcgcgcctt tgcggccggc tactctgtgg agttcgttcg caggatatta
     3301 aggccggcgg gcttttcgtc ctccctgggc gcgggcgctt ccggttgcgg tcgaatcacg
     3361 ttcaggtcgc ggacagaccc gctcgccgga ccggatcgcg ccgcgccgcg gattttcagc
     3421 tcggccttcg gcccgatcac gcgcggttcg ttctcgcccg atgccccgga ccgaaacgcc
     3481 aggtgcaatg ccccccagcg cggcaggtcc cacgtcacgc gcgccggcca gggctcccgc
     3541 gggaactggt tgagtcgcgg cccggccggc gccggctcca cggccgccgc cgctcccccc
     3601 tcggcccccg ccagcggccg gccgtaggca tcctcggcgg gcatcgcttc gtcgccgcgc
     3661 atgttcgcat acagccgcgc cgccatcccc acgaaaaccc agtagtgacc gatcatcaca
     3721 taatgcgtga accggtcgcc gaagatattg gccaccatca gccccaccgt ggcaatcatc
     3781 agacagaagc cgaagtagcg gtcgaacacg tgcggtccgc cgcgccacaa ccgcaggcac
     3841 atcatcagga tcacgatcca gaccgtcatg aacgccgcga agccgacgac cccctgttcg
     3901 gccagcgtgc gcagatacat attgtgggtg tccattcggc gcgggttgag cacctggaac
     3961 gacccgacgc catggccgaa gatcggcgac tgctcccaca gctcggtggc gagcttccag
     4021 aagtccttgc gcgattgcgc cgattgctcc aggcgccccg cctcatcctc ggtcttcagc
     4081 caccgttcgc gcacggaata cggaacccac aacggattgg tcacgatcgc gaacaccagg
     4141 acgacgatca gcggacgaaa cttgaggcag gcgaccagaa tcaccgccag gccgaaggcc
     4201 agctgcgtcg cgcgcgagta ggagtaaagg atgccatagg catacgccga cgccgccagg
     4261 gcgaacagcg cctgctccca gcggcgacgc ggcagaaaga acagcagccc cagcatcagc
     4321 gcgctgtaga cgaaaaaggc gcccagctcg ttcgacccca gaaacatgaa cgggccgcgc
     4381 acgcgcatgt cgtcgtcgaa gtggaaactc cgcaccgagc gcagctgcgt gaaaaacacg
     4441 cggaaaatgt agggtgcggc cagcgcgatc acgatcaggc actggcggat cttgcgccgc
     4501 gtgtcgagca gatgcgcggc gagccagaac agcaggaacc cgttgaagaa ttgaaggatc
     4561 cgcgtcaccg ccagcgaact gggcgcaaac ggcgaatcga tttcgggcag gcccgaccag
     4621 taggccagca tcatcccgag gtaagtcagg ccgaacagca cgatcagcgg tagattgagc
     4681 ggcgagcgca tcagcagcgg ccggcgccgc gtcatccgcg cgagcaccca gatcaccacc
     4741 atgagcgcca ttgtgagatt ggcgtagttg atgcccgtcg ggcccttggg cagcttctcc
     4801 tgcaggttct gcagcggaac catgaagacg taaaggtaaa agagccaggt catggaggac
     4861 ggtgcgtttg gaatttcggc ggctgggcgc aagcccggcg cagccgcgcc gaaccggggc
     4921 agcgggctgt ccggaccggc cgcgccacac ccgttctgtt ccagtcagcc tatacgtctc
     4981 aacggccgga gcctaggaca acttgagcga tgggcgttcg tcccccgaag gtcgtctctc
     5041 gtctctcgtc ctcgtcctcg tagtcgcccc catcggcccg cccatatcct tttccctcca
     5101 atcgaaagtc ggctttactc acgtcgaagg cggaaagtgt ttgttgccga acgcatcgag
     5161 gaccggcagc gcggcgcgcg cgcgacggat ccgctcggcc tcctccagat gccgccgcat
     5221 ctcgtacggc ttttgttcga ctcgcgcgat ctcgctcagc agaatgtggc cctgcatctg
     5281 atactccggg taatcgagcc acatcaatcg attcgcgaac tttcgcgcct gttccagttc
     5341 gccggccgcg aacgcctgtg ccccgcgcgt gagtgcgaag ttgccgaaaa tcgaatcgct
     5401 gtgccacatc gaatgcaatc gcgagacgtc gtagacgttg accagagcgc ccgccgccag
     5461 caggcccgcc gcgatccccg ccagcgcggg cagccgccgg cgcgccagcc cgcgcccccg
     5521 gatcagttcg acggcgcgtt gcgcgagaag ccccgcgaac aggaacagca gcggcgtgat
     5581 cgtcagccgg taacggcccg aaacgtagaa caggatctgc gcaaagagca cgacggcgaa
     5641 catcccgagc agcagggaaa atcgccgcgg gtggaacagc gccaggacca gtccgacgag
     5701 gcccagcggc gcgacgagcc gatacgtcac cggatcggcg tgcagcggcg gcgacaaccg
     5761 gctgtagaga tggaaattgg tcagatcggg aaactccagc caggcgaaga actggcgcag
     5821 cttgcgcgcc gtcaacgcca gcacgcgcgg atcggtcggc gccagcagct cgctttcggg
     5881 aaaaccctcg ggataataaa agtaaccggg cgaatcaacc gaccatgaga actgccagat
     5941 gatgttcgat tcgccgacga cgaaccgcgg ccgcccggcc acccgcgagt tgtagatgct
     6001 gaccgggatc agcgtgatca gcgagacgcc ggcgatcagc acgagggcgc cgagccgccg
     6061 ccgccagacg cgtccgcggc tcatccccgc cgcccacgcc gccggcgccg ccggcagcca
     6121 caccgccagc gccaacccga tcaggcagag attcgcccgc agcatcgtca ggccccccag
     6181 cgccaggccg agcaacacgg gaatccgcca ggcggaggct tgtggatggg tctggtaccg
     6241 cgcccccaga taaaggatca gtgccacgac gaccgggttg agcgcgtccg ccgtcatcac
     6301 ggtttcgtag tagacggcgg gcgcatagag cgccgccagc gcccccgccg ccgtggcgcc
     6361 cggctcgccg atcagccgcc gcgccgccag atacatcaac gccacgccgg cgacccccgc
     6421 gcagagctgc aacccataca tccaccagaa ccgcccgccg gtcgccttca tcacccccgc
     6481 gagcaggtaa acgtagagcg gcccgtgctt gaaatagccc tcgggcgtgc cgccgcgctc
     6541 gagcacctgg cgcgcctgct gcatgttgtc gatcaggtcg ccgttgggga acgtctccgg
     6601 gtcgaacagc gggttttcct cgctgatcag atagggcgcc gagaccagcc gcagcatcac
     6661 cgccagcgcg atcagccacc cgatcgggcg gcgccagacg gggggccgtg gcgccgacgc
     6721 cggtaattcg ctttcgctcc cgttctcgcc ggccgcgggc atggactccg gccggagggg
     6781 aatggccgcg cgttgcggtt cctcacgcgg ggtgttgcgt cgtcccatgc aatgcatccc
     6841 ggccaggttt ctcccgtttc agccgcgctc gcggagctcg ctgtcgcaga acagcagcgt
     6901 ccccgccacg gccgaaacgg gaatgaacag cgcattgagc agcgggaccg gcatgatcac
     6961 gaacgccatc gtccccaggc cgagcgtccg ccagcggttg cgccgcagaa attcgcgccg
     7021 ccgcgcataa tcatagtagc gccggtccat gctatatgag gtgaactcca gcgccaggaa
     7081 ccggatcgtg atcgcggccg aggcgacggc cgccgccgcc gacccgatca ccggcgccag
     7141 atgcagcggc aacgtgcaga tgaacaacag cagcgtcacc cagaacaggc gcaggctggt
     7201 ggccaggccg acccgtaacc cgcgccagag cgccttgagc gagaacgatt cctcgaccgt
     7261 cccgccgccg tagatcacct cgaccttctc cgacaggacg tcgttgaacg gcgaagcgat
     7321 cagcgccgcc atcggcacga acgcgaagag cgtcgtcagc cccagcgcga tccagatcag
     7381 cgcgcccagc aggtaaccga acagatcggc ccagaaaccg ccctgggcaa agaacgcccg
     7441 ctccagccag ccgccggcga aatgccagac ggcccaggca acgccggcga agagcagaac
     7501 gttgatcgtg aacgggatga gcgcccagat ccagagcccc ggatggccga gcaggaagcg
     7561 caggccgcgc cagacgtcga ccgcgccgcg gagaaactca gccggcatct cgcgcgagat
     7621 cctgaggaag tcgcggcgtc acgggtcggt cttctgcgtc agcttccgcg ggtcggccag
     7681 ttccgaggcc gactcgacga ccttctcggc gatggccaga tggtaaaact ccgtatatcc
     7741 gcacagcccg caggtcacgg ccaggtagct gttggccggc agcggcagca gattcaccat
     7801 cgcccggccg acggagacct cctgcacttg ggcgccgtgc ccgtggcatt tcggacagac
     7861 gaattctcgg gccagttggt ctttgcagcc gtggcgcatc cgagccctgc ctgatccggc
     7921 gtgaggcggc cggggagtac tgaacggtac gatgccagag aacgggcggg gtttcaattg
     7981 atgatcaccg aggcggggaa agggagaacg cttggatgga ccgttggggg cgatcacgag
     8041 aacgagaacg aggacaagag acgaggacgg caagaaaacc agacgggcca ttcccccacg
     8101 tcgcgggaat ggcccgtccg aaacatcgac cgctcggcgc gtctccctag cgcggtccgg
     8161 ccagcggaat cgtggacccg cattgcgaca ccgacacgtt cttctcgaac acttcgcgcg
     8221 gcaccgacgg gccctgacgg ctg
//
LOCUS       DAOPCO010000176         8128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8137_length_8128_cov_5.008795, whole genome shotgun sequence.
ACCESSION   DAOPCO010000176 DAOPCO010000000
VERSION     DAOPCO010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8137_length_8128_cov_5.008795"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLS90_11815"
     CDS             <1..729
                     /locus_tag="PLS90_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03135.1"
                     /translation="PVSVFATGKRQFYTPETARRMGLRSHHERCRTCPEKDACGFYFD
                     LGQHPLIRALYLDCEEFDGYFRDRCVFRPEISIEDTMHVQVRYANDATMSYSLVAHAA
                     WEGYTLAFNGTQGRLEHKCEESIYVSGDGTVPGALVGDGTYTRIFPLRKPAYEVPIWQ
                     SEGGHGGGDKPLLEDLFLPVRPADPYLRAADHRSGAYSILTGVAANRSMETGAPVEIA
                     SLVRGLQSPDFPPMPDRRKKLAMP"
     gene            841..925
                     /locus_tag="PLS90_11820"
     tRNA            841..925
                     /locus_tag="PLS90_11820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:875..877,aa:Leu,seq:aag)
     gene            complement(1018..1173)
                     /locus_tag="PLS90_11825"
     CDS             complement(1018..1173)
                     /locus_tag="PLS90_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HPK03136.1"
                     /translation="MSCFRVLLCVIFPPLAVIDKGCGSFLIVCLLTVVGWVPGVLGAL
                     IICREAD"
     gene            complement(1170..1598)
                     /locus_tag="PLS90_11830"
     CDS             complement(1170..1598)
                     /locus_tag="PLS90_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03137.1"
                     /translation="MQPDADPQTLRKIAQTLGADRLRRHIFLCCDQSKPKCCNKEAGL
                     RSWEFLKRRLAELGLDGAGGIYRSKVNCLRICQGGPIALVYPEGAWYHSCTPDVLERI
                     IQEHLIGGRVVEQYLILRHAPDGRGATERGPIGEASEAPR"
     gene            1834..2052
                     /locus_tag="PLS90_11835"
     CDS             1834..2052
                     /locus_tag="PLS90_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03138.1"
                     /translation="MLIEHDLMPELTAPQLAGLRNDLACLGSLPPSTQWELLALIERL
                     QESRLQAVDALRVARETVGQALALLHPE"
     gene            2390..2860
                     /gene="msrA"
                     /locus_tag="PLS90_11840"
     CDS             2390..2860
                     /gene="msrA"
                     /locus_tag="PLS90_11840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HPK03139.1"
                     /translation="METATFGAGCFWGVEATFRRTKGVKATAVGYMGGWKKSPTYEEV
                     CTDQTGHAEVVQVQFDPNQISYGELLNVFWENHDPTQLNRQGPDFGKQYRSVIFYHTN
                     EQKQTAEASKQALAESGRHRRPIVTAIEPAAEFWRAEEYHQRYLEKRGLATCHL"
     gene            complement(2870..5680)
                     /locus_tag="PLS90_11845"
     CDS             complement(2870..5680)
                     /locus_tag="PLS90_11845"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPK03140.1"
                     /translation="MQTDRPRPAAAPPAPPPRALRAWLPALAAVLLFLPPRPAAAHSF
                     SADDVQHLWTVTVKEGVIELELDCLFQGDPAAALRTAIDTDGDANGRLSAAEARQYAL
                     ARHDEWTSAVALHQETRPLKLTPLYPPRVELLDADHTGPAPLRLRLAWFTRHDEPALY
                     YLDDLLWLDRPAMLSLATAGDPPPRIVQPAGAHQHARPTEPRYLAFQIEPPPWDPDAR
                     QSRFDALGAAELIGRLVSDQAYHLLAPGHQAELEELNRELHESMELRTLYHVNRGTST
                     FPPAARELWDKVLRLIEQSGRLIEAPLEPGHPALPLAGPFELPGDVGALVIRARADGL
                     DETRFQIHDFSFSAYDIMEHAKIDLPATGTTYILLNLRDVPAGGVRTVFELYTSRRAM
                     PWSLPIEVVPPAPARLALRVDDDRDQPGPFMVRLNWLLDGSDRKPPNVVELADQFEGQ
                     GYATSLRTSHMPGVMNQSYWCVPGPFEMPIPPGRYGLSVRRGIEYVPVTRIFSAASGE
                     RLELTVNLRRWTDMPARGWWSGDDHAHLRIQTDEDAARALRWAEAEDVHVVNVLEMSS
                     IARRHFVQRGFGPPFRARTGPYALVPGQETPRTHNELGHVLALNILDLIHFADRYFLY
                     DLVFDEAHRQGGLTGYAHVVNLPRFEVDRDMSLNIPKDKVDFLEVLQRGELGTTVSYD
                     FLNLGFKLVFAAGSDVPYGGSLGEVRTYVHTGNRKTLDVDAWFDAFRQGRTFVTNGPM
                     LELEVEGAGPGEELRIEAPRPLSVKARAWGHAGYTVPARLEIVRLGEVAADIRSDDDQ
                     REELTLRARVDSGDGCWIAARVTGHDGTVAHTTPVWVVRPPLRFWKYEAAPGLIAQRR
                     ARLDEIEQLVHHARRLEAAGALQGWTARGFPWLIEEDIQRELARQGDDLLERVAAARA
                     IYDELLRVHAREAPLRAGSSAR"
     gene            complement(5670..7079)
                     /locus_tag="PLS90_11850"
     CDS             complement(5670..7079)
                     /locus_tag="PLS90_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03141.1"
                     /translation="MSRGFLVFPLMLLLLNAAGARAPFVPDEFDQGRLGPAWRRAPAA
                     RGDVAVQGGALVLRPAGGTERSYSRHDTLWIEQPAPAGADWELITRIDAFDPVLNDDG
                     LAPPWSECGLQLHQDERHWFGIWLNTNQTGHRLDVTCGFQMEPGEIQGKPFDPFFCGD
                     MAVWDIRRLPVWLRLRKTAHGYFGAYRVEGEPWRNLGPFARNPDSPADGFLRGETVRL
                     YAAAPAADVPPPVCRIGFIRFRELEPLPRAGGSDGFDDERLDRARWGVYEGAPSAATL
                     AESGGALHLTPRMYQEINTNFDTAPCVYQPAPAAGPWRASVKAGPTDLRAHQAWSAYG
                     LMLWQDQNHWILVRNQVDDGRRLRSQALRHEGRRLWVETRDYLAGELPPYLCIERWGD
                     AYRCGTSQDGRNWEYLNDAGEFVTERPFDNAQLQLFATRPTEDDAGATLTARFDAFHI
                     EPLGEPAAGAEGLSSPHAD"
     gene            complement(7297..>8128)
                     /gene="lepB"
                     /locus_tag="PLS90_11855"
     CDS             complement(7297..>8128)
                     /gene="lepB"
                     /locus_tag="PLS90_11855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPK03142.1"
                     /translation="APPQPAAAPPAPREPLTRRLRDLADELVFVFVIVMFVKLFLVEL
                     YKIPTGSMTPTLLGGHIAHVDLNEDGRRDIIYFDQDQSYALAFVWNGERYVARPDLNP
                     DGPFLDRLIRAGRTGKQFDRILVNKLAYWFRGPRRGEIVIFKVPRSIYRREAPIYIKR
                     LVGEPNDLLTFDPAGRLIVNGRLQEEPDFFKTQRYNPVIRVTTNHFERIPDIHYLPED
                     RHSVRLESIAIPEGHYYVFGDNTAGSLDSRYWGGVEQDHFKGRAFLRVWPLTQFNYLK
                     "
BASE COUNT         1318 a         2530 c         3029 g         1251 t
ORIGIN      
        1 ccggtctcgg tgttcgccac cggcaagcgg cagttctaca cgcccgagac ggcgcggcgg
       61 atgggcctgc gcagccatca tgagcgctgc cgcacctgcc cggagaagga tgcctgcggc
      121 ttctacttcg atctgggcca gcatccgctg atccgcgcgc tctacctcga ttgcgaggag
      181 ttcgacggct attttcgcga tcgctgcgtc ttccgccccg agatttcgat cgaggacacg
      241 atgcacgtgc aggtgcgcta cgccaacgac gcgacgatga gctactcgct ggtggcccac
      301 gcggcgtggg agggctacac gctggcgttc aacggcacac agggccggct ggagcacaag
      361 tgcgaggaga gcatctacgt cagcggcgac gggacggtgc cgggcgcgct cgtcggcgac
      421 gggacgtaca cccggatctt tccactgcgc aagccggcgt atgaagtgcc gatctggcag
      481 tccgaggggg ggcacggcgg cggcgacaaa ccgctgctgg aggatctgtt cctgccggtc
      541 cggccggccg acccgtatct gcgcgccgcc gatcatcgtt cgggcgccta ttcgattctg
      601 acgggcgtcg cggccaaccg gtcgatggag actggcgcgc cggtggagat tgcgtcgctc
      661 gtccgcgggt tgcagtcgcc cgattttccg ccgatgcccg atcgccggaa gaaactggcg
      721 atgccgtgag accacggaag atggagcgcc gttgcgcgtc gccccaccag ggcggcgatc
      781 gcggtgataa cccgttgact cagcggccga cgttgctatg atctaggttt cgccccccgt
      841 gccgggatgg cggaattggc agacgcgcag gactaaggat cctgtggggc aacccgtggg
      901 ggttcaagtc cccctcccgg caccaatctc aataaaatca gtcacgcgag agaatagaga
      961 atcgagagag gccccggcgg ggcctttttt cgtgggtccc cgtgcgggcg aggcgcttca
     1021 atctgcctcg cggcagatga tcagcgcccc caggacgccg ggcacccagc cgacgaccgt
     1081 cagcaggcag acgatcagaa acgacccgca ccccttgtcg atgaccgcca gcggggggaa
     1141 gatcacgcac agcaggacgc ggaagcagct catcgcgggg cctcgctcgc ttcgccgatg
     1201 ggtccgcgct ccgtcgcgcc gcgaccgtcg ggcgcgtggc gcaaaatcag gtattgctcg
     1261 accacccggc cgccgatcag atgctcctga atgatccgct cgaggacatc gggcgtgcag
     1321 gagtgatacc aggccccttc gggataaacg agcgcgatgg ggccaccctg gcagatgcgc
     1381 aggcagttga ccttcgagcg gtaaatgccc cccgcgccat cgaggccgag ctccgccaga
     1441 cggcgtttca ggaactccca gctgcgcagc cccgcctcct tgttgcagca cttcggtttg
     1501 gactgatcgc agcagaggaa gatatgacgc cgcaggcggt cggcgccgag cgtctgggcg
     1561 atcttgcgga gggtctgcgg gtcggcgtcg ggttgcatgg tcgctagtta agcatgaaag
     1621 cggcgcgaag ggaagccggg cgggggcgcg gggtgcccgc tggcggagaa tggtgtgggt
     1681 tgtcgggtgc gcggcgccgg tgatttttgc gtgacacggg ccgaagaacg gaggcacgat
     1741 ggcgccattg cagtttggtt gatgccggat gcccgccggg atgggcaagc cggttcgcgt
     1801 tccacgttca ggttgccgcc ggaggtcctg ccgatgctga tcgagcacga tctgatgccg
     1861 gaattgaccg cgccccaact ggccgggctg cggaacgatc tggcctgcct gggatccctc
     1921 cccccatcga cacagtggga attgctggcg ttgattgaac ggctccagga gagccgcctg
     1981 caggcggtcg acgccttgcg ggtggcgcgc gagacggtcg gtcaggccct cgcattgctg
     2041 catccggaat aattcccgcc ccgggaccct cgccatcaaa tcggtattcg cgctgtcatg
     2101 ttcacgctga gaacgcgact gatccgcacg gggcggtgac acgccgcgcc gggcatggta
     2161 gactgaacag gaggaggccg gagccatgaa ccccgtcttg ctggtcggca tgctgcttgc
     2221 cgcaacgctc gccctgacgg gatgtcccgc caatccggcg gccctcgccg acggaccggc
     2281 gaccgcaacc gaaccggcgg ggtccgaccc cgcggcggcc gatgaaccca cgaccggcgc
     2341 cgcgtccgtt gcaaccgtcc aacctgaagt tccccaagga gagacgacca tggagaccgc
     2401 gactttcggc gccggctgtt tctggggtgt cgaggcgacc ttccgccgga ccaagggggt
     2461 caaggccacc gccgtcggct atatgggcgg ctggaagaag agcccgacct acgaagaggt
     2521 ctgcaccgat caaacgggcc acgccgaggt ggtgcaggtg cagttcgatc ccaatcagat
     2581 ttcgtatggc gagctgctca acgttttctg ggagaatcac gacccgacgc agctcaaccg
     2641 ccaggggccg gacttcggca aacagtatcg gtcggtcatc ttctaccaca ccaacgagca
     2701 gaagcagacg gccgaggcgt cgaagcaggc gctggccgaa tccgggcggc atcgccggcc
     2761 gatcgtcacc gcgatcgaac cggcggccga gttctggcgc gccgaggaat atcaccagcg
     2821 ctacctcgaa aagcgcggcc tggcgacctg tcacctgtag cgggatgagt cagcgggcgg
     2881 aggaaccggc acgcagcgga gcctcgcgcg cgtggacgcg cagcagttca tcataaatcg
     2941 cgcgggcggc ggcgacccgt tcgagcaggt cgtcgccctg gcgggccagc tcgcgctgga
     3001 tatcctcctc gatcagccag gggaagccgc gcgccgtcca cccttggagc gcgcccgccg
     3061 cttcaagccg gcgcgcgtga tggacgagct gttcgatctc atcgagacgg gcgcggcgct
     3121 gcgcgatcag gcccggcgcg gcttcgtatt tccagaaccg cagcggcggg cgcacgaccc
     3181 agacgggggt cgtatgcgcg accgtgccgt cgtggccagt gacgcgggcg gcgatccaac
     3241 agccgtcgcc ggagtcgacc cgcgcgcgca gcgtcaactc ctcccgctgg tcgtcatcgg
     3301 agcggatgtc ggccgcgacc tcgccgaggc ggacgatctc cagccgggcg gggacggtgt
     3361 agccggcgtg gccccaggcg cgcgccttca ccgatagcgg ccggggggcc tcgatgcgca
     3421 gttcctcgcc gggcccggcg ccctcgactt ccaattcaag catcggtccg ttggtgacga
     3481 atgtgcggcc ctggcgaaag gcgtcgaacc aggcgtcgac atcgagagtc ttgcggttgc
     3541 ccgtgtggac gtaggtccgc acttcgccga gggagccccc gtaggggacg tcgctgccgg
     3601 cggcgaagac cagtttaaag cctagattga ggaagtcgta gctgacggtc gtgccgagtt
     3661 cgccgcgctg gaggacttca aggaagtcga ccttgtcttt ggggatgttg aggctcatgt
     3721 cgcgatcaac ctcgaaccgc ggcaggttca cgacgtgcgc gtagccggtc agccccccct
     3781 gccggtgcgc ctcatcgaac acgagatcgt agaggaagta gcggtcggcg aagtggatca
     3841 ggtcgaggat gttgagggcg aggacatggc ccagttcgtt gtgggtgcgg ggggtctcct
     3901 ggccggggac gagggcgtaa gggcccgtcc gcgcgcggaa ggggggcccg aagccgcgct
     3961 ggacgaaatg gcggcgggcg atggacgaca tctccaggac gttgacaacg tggacgtcct
     4021 cggcctcggc ccagcggagc gcgcgcgcgg cgtcctcgtc ggtctggatg cgcaggtggg
     4081 cgtgatcgtc gcccgaccac cagccgcgcg cgggcatgtc ggtccagcgc cggaggttga
     4141 cggtcagctc gaggcgttcg ccgctggcgg cgctgaagat ccgggtgacg gggacgtatt
     4201 cgatgccgcg gcggaccgac aggccgtagc ggccgggcgg aatcggcatc tcgaacggcc
     4261 ccggcacgca ccagtaggac tggttcatca cgccgggcat gtgcgaggtg cgcaggctgg
     4321 tggcgtagcc ctggccttcg aactggtcgg ccagttcgac gacgttgggc ggcttgcggt
     4381 cgctgccgtc gaggagccag ttgagccgca ccatgaaagg cccgggctgg tcgcggtcgt
     4441 cgtcgacgcg cagcgcgagg cgggccgggg cgggggggac gacttcgatc ggcaggctcc
     4501 acggcatggc gcgacggctg gtgtagagtt cgaagacggt gcggacgccg ccggcgggga
     4561 catcgcgcag attgagcagg atgtaagtcg tgccagtggc gggcagatcg attttcgcgt
     4621 gctccatgat gtcgtaggcc gagaacgaga aatcatggat ttggaagcgg gtctcgtcga
     4681 gcccgtcggc gcgggcgcga atcacgagcg cgccgacgtc gcccggcagc tcgaacgggc
     4741 cggccagcgg cagggcagga tggcccggtt cgagcggcgc ctcgatgagg cggccggatt
     4801 gctcgatcag gcgcagaacc ttgtcccaca gctcgcgcgc cgccgggggg aaggtcgacg
     4861 tcccccggtt gacgtgatac agcgtgcgca gttccatgct ctcgtgcagt tcgcggttga
     4921 gttcctccag ttcggcctga tgtccgggcg cgagcaggtg gtaggcctga tccgagacga
     4981 gccggccgat gagctcggcg gcgcccaggg cgtcgaagcg gctctggcgc gcgtcggggt
     5041 cccagggcgg gggttcaatc tggaacgcca ggtaacgcgg ctcggtcggc cgcgcgtgct
     5101 ggtgcgcgcc ggcgggctgg acgatgcgcg gcggcggatc gccggccgtc gccaggctca
     5161 gcatcgccgg gcggtccagc cagagcaggt cgtccaggta atacagcgcc ggctcgtcgt
     5221 ggcgcgtgaa ccaggccagc cgcagccgca gcggcgcggg gccggtgtgg tccgcatcga
     5281 gcagctccac ccgcggcggg tagagcggcg tgagtttgag cggccgcgtc tcctgatgca
     5341 gggccacggc gctcgtccac tcatcatggc gggccagggc atactggcgc gcttcggctg
     5401 ccgaaagccg gccgttggca tcgccgtcgg tgtcgatcgc ggttcgcagg gcggcggcgg
     5461 ggtcaccctg aaagagacaa tccagttcca actcgatgac gccctccttg acggtgacgg
     5521 tccagaggtg ttggacatcg tcggccgaga aggagtgcgc ggcggccggg cgcggcggga
     5581 ggaacagcag caccgccgcg agcgccggca gccacgcgcg caaggcacgg ggcggcgggg
     5641 ccggcggggc ggcggcgggg cgtgggcggt cagtctgcat gcggcgaaga gaggccttcc
     5701 gcgccggcgg cgggctcgcc caggggctcg atgtggaacg cgtcgaaccg ggcggtgagc
     5761 gtggcgccgg cgtcatcctc ggtcggccgg gtggcgaaaa gctggagctg agcgttgtcg
     5821 aacgggcgtt cggtcacgaa ttcgcccgcg tcgttgagat actcccagtt gcggccgtcc
     5881 tggcttgtgc cgcagcgata ggcgtcgccc cagcgctcga tgcacaagta gggggggagt
     5941 tcgccggcga ggtaatcgcg cgtttcgacc cacaggcggc gaccctcgtg gcgcagcgcc
     6001 tggctccgca gccgccggcc gtcatcgacc tgattgcgca ccaggatcca gtgattctga
     6061 tcctgccaga gcatcaggcc ataggccgac catgcctggt gcgcgcgcag atcggtgggt
     6121 ccggccttga ccgaggcgcg ccagggtccg gcggcgggcg cgggctggta gacgcacggg
     6181 gcggtgtcga agttggtgtt gatctcctga tacatccggg gggtcaagtg gagcgcgccg
     6241 ccggattcgg cgagggtcgc ggcgcttgga gcgccttcat agacgcccca gcgggcgcgg
     6301 tcgaggcgtt cgtcatcgaa gccgtcgctt ccgcccgcgc gcggcagggg ctccagctcg
     6361 cggaaccgga tgaagccgat ccggcagacg ggcgggggga cgtcggcggc gggagcggcg
     6421 gcatagagcc ggaccgtttc gccgcgcaga aagccgtcgg cgggcgagtc ggggttgcgc
     6481 gcgaacggcc cgaggttgcg ccacggctcg ccctcgacgc gataggcccc gaagtagccg
     6541 tgcgcggtct tgcgcaggcg cagccagacc ggcagccgcc ggatgtccca gacggccatg
     6601 tcgccgcaga agaacgggtc aaacggcttg ccctggatct cgcccggttc catctgaaac
     6661 ccgcaggtca cgtcgagccg gtgaccggtc tggtttgtgt tgagccagat gccgaaccag
     6721 tggcgctcat cctgatggag ctgcaagccg cattcgctcc aggggggcgc cagcccgtcg
     6781 tcgttgagaa cgggatcgaa ggcatcgatg cgggtgatga gttcccaatc ggcgccggcc
     6841 ggagcgggct gctcgatcca gagggtgtcg tggcgcgaat aggagcgctc ggtgccgccg
     6901 gccggccgga ggacaagggc tccgccctgg acggcgacgt cgccgcgggc ggcgggggcg
     6961 cgccgccatg ccgggcccag gcgtccctgg tcgaattcat cgggaacgaa cggcgcccgc
     7021 gcgccggcgg cgttgagcag gagcaacatc aggggaaaca cgagaaaacc gcgcgacatc
     7081 agcgtgcttt ccggtttgag tgggggtgca ttataggcga gggacgcggg cgatgccacc
     7141 gccgcgcgcc gcgcttggcg gatgggggaa ggccatgcgg cggcgccggt ctgacgcgga
     7201 tgaccgtgcc gagccggacc gatccaaagc ggcgtcgacg accgctgcgc gggctctggc
     7261 gccgcactcc aggaggcgct gacgactcga aggcgactat ttcaggtaat tgaactgcgt
     7321 cagcggccag acgcgcagga aggcgcggcc tttgaaatga tcctgctcga cgccgcccca
     7381 gtagcggctg tccaggctgc cggcggtgtt gtcgccgaag acgtagtagt ggccttcggg
     7441 gatcgcgatc gattcgagcc gcaccgaatg gcggtcctcg ggcaagtagt ggatgtcggg
     7501 gatccgctcg aaatggttgg tcgtgacgcg gatgaccggg ttgtagcgct gtgtcttgaa
     7561 gaaatcgggt tcttcctgga gtcggccgtt gacgatcagg cggccggcgg ggtcgaacgt
     7621 cagcaggtcg ttgggctcgc cgacgagccg cttgatgtaa atcggcgcct cgcggcggta
     7681 gatcgagcgg ggaaccttga agatgacgat ctcgccgcgt cgggggccgc ggaaccagta
     7741 ggccagtttg ttgaccagaa tccggtcgaa ctgcttgccg gtccgccccg cgcggatcaa
     7801 ccgatcgagg aacgggccgt cggggttcag atcgggacgg gcgacatagc gctcgccgtt
     7861 ccagacgaag gccagggcgt agctctgatc ctgatcgaag tagatgatgt cgcgccggcc
     7921 gtcttcgttc agatcgacgt gggcgatgtg gccgcccagg agcgtggggg tcatcgagcc
     7981 ggtgggaatc ttgtacagtt cgacgaggaa cagcttgacg aacatgacga tgacgaaaac
     8041 gaagacgagc tcatcggcga ggtcgcgcag ccgccgggtg agcggctcgc ggggggcggg
     8101 gggagcggcg gctggttgag ggggggcg
//
LOCUS       DAOPCO010000177         8114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8154_length_8114_cov_4.428837, whole genome shotgun sequence.
ACCESSION   DAOPCO010000177 DAOPCO010000000
VERSION     DAOPCO010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_8114_cov_4.428837"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLS90_11860"
     CDS             <1..327
                     /locus_tag="PLS90_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03143.1"
                     /translation="VGEQLEQAVDTTARYVGQQTETLAQHIDAELNALRARLNEYEAR
                     HAGDDDAARREWQSTRAGLEARMNRLDEEAGRLADQADDKVEAARRDLEAGMADLRRA
                     IDDLEN"
     gene            381..740
                     /locus_tag="PLS90_11865"
     CDS             381..740
                     /locus_tag="PLS90_11865"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HPK03144.1"
                     /translation="MRSRLHAAAALLLLAISPLVVTSCYGRFPLTHAVYDFNGEIGND
                     AGNAEKWVESALFWVFVILPVYQLASLGDAIIFNVIEFWTGDTLRIGAAPGHGALAGV
                     ADPDGAARLAAAPQPGP"
     gene            complement(763..1578)
                     /locus_tag="PLS90_11870"
     CDS             complement(763..1578)
                     /locus_tag="PLS90_11870"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK03145.1"
                     /translation="MNRILSDEQWFQFFQQGYLRLGRVLDDAELKALQTRIDQIMLGE
                     ADVDYDRMLMQLDSETGRYEDAPAQVLGFKGPTLNYRKIQNLEMDPVFLRFMQKPIFR
                     EICAEVYGAHASISVYRAMFMNKPSRRGTVLPWHQDGGEQWELDRDPLVTLWTALDAA
                     TIANGCVQVIPGTHRLGLLSDFGHTITPEQEREHCPEDRIIYLELDPGECVLLHNLLL
                     HRSDVNGTDQSRRGFSVCYMDARTRTTKGESFPVIFGKGALDPEQLRMAKAGS"
     gene            complement(1757..3130)
                     /locus_tag="PLS90_11875"
     CDS             complement(1757..3130)
                     /locus_tag="PLS90_11875"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HPK03146.1"
                     /translation="MDATRMIPAAETSRDAMDPAAEAGGHAVFIETYGCAFNAADGEA
                     MAGVLQRAGHRVVSDPSAADVIIVNTCTVKDRTYLNFEKRFRRLKAAALAGEGPRLVV
                     AGCIPKAYERSDLLAGVAALGPDAIGRVDEVVEQAMRGGAPHVLRGERQRGAPRERLP
                     TARRNPLVEILPIASGCLSACSFCQTRLARGRLRSFRPSDLVEKARRGIDEGVREIWI
                     TSQDVGAYGREAGYTLTGLVGRIAELPGDFRIRLGMTSPQWVSAELEAWLDLLDHPRL
                     FRFLHIPVQSGADGVLRAMGRENTVAEFEAVCEAMLARHPDLALMTDLIVGFPTETEA
                     DFAETLALVERVPFAAVNRSRFSARPGTAAARLTGLPGAVVTERSSRLNVAVRVTTRR
                     WHERLVGRRERVLTFESDPAGRRTLAHNGAWRPVVLPGVRAVGEWLDVEYVGAADFHL
                     KGRPVTI"
     gene            3467..4738
                     /locus_tag="PLS90_11880"
     CDS             3467..4738
                     /locus_tag="PLS90_11880"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HPK03147.1"
                     /translation="MRLAVRLPVALLLLALLAGCSWLRHRPTDTPSLASPDELRRAYS
                     LVSDPSRYYDKVHGAWLGKLIGLALAQPVEGWYKEGIRERAEEVGAYPVQGYFPSNFR
                     GIPGCEPWLYGNLAGSPSNDDVEQMLLALVTLRERGVDPTPRDIAEIWDKYLPYGNVA
                     SGVALYNIRRGIWPPESATTDNPYQNRIGGHMRVDIWGMVAPGCPALAADLAERDASL
                     AHTGEGIYGARFVAAAVSLAMFERDPKTILTQALEFIPADCEYATYVRDVIGWHAQYP
                     DWEDAYAELDRKYGFYPDGTREREYDDPRFDRSIPRYQWNDARLGLATLNGAACALAL
                     LYGEGDFARTICLSTMIGYDTDCNAGTVGAIVGASIGASQIPPYWIEPLQDTYRSHVK
                     PIGKEASISAIARETALYGYQVIASRLIEHP"
     gene            5175..6416
                     /locus_tag="PLS90_11885"
     CDS             5175..6416
                     /locus_tag="PLS90_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03148.1"
                     /translation="MIATDITDPTTAAAEPGFNPPADPATTAANETAVLFVDDDANVL
                     AAYRRMLSQRFRLETAASGAEGLQRLREGGPFAVVVSDLQMPAMNGIQFLSRAREVSP
                     DTVRMMLTGQADLSRAIDAVNEGNLFRFLTKPCPADMLAKAVEAGLRQYQLARAEKDL
                     LQETLRGAVKVLVDVLSLVNPPAFGRAARVRRLARQVAEELRLEDAWQVEIAAMLSQL
                     GCVTVPEKTLHKHCLGQDLTPEEWRMVRRQARIGADLLRCIPRLEAIAEMIARQDVPW
                     REQRERGAAGEAPGGADVLKAAVDLDTLISSGRHFAQALAALRGRRGEYHPAVLTAIE
                     RVQKLDAGYQIRQVALDELRPGMILAQDVATGGGMLLLARGQEATESVCQRLCNMARI
                     YEGLPTAVKVMVPRGQDNEGS"
     gene            6822..>8114
                     /locus_tag="PLS90_11890"
     CDS             6822..>8114
                     /locus_tag="PLS90_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPK03149.1"
                     /translation="MLSDLDFQQVEWTPAAPPEQGESDTLLLTDSDPERGRRIADRFA
                     RQGYRCQLAVDHPDALRKLIAEETALVLLVCDGSAAEIYPWLDAIGQARNAPVQLVLV
                     PEERDAQRVVIQADAFDYLVLSRLDDQGYWEPILQRALRQHHANREAFAVRRILRHNT
                     VIFDASPDMILVLDGNRRILHCNLAVENYTGRARRELIGERAHQVLCGDHHPAEACPL
                     GGGLHEGDRTRPICRMQRRTQTFQMRSIPLFTRDGKVWGTMEILQNVTELIQAEQALN
                     HRLEIEKLIAEISVEFAHNEDMDQAIHRTLARIGLWSQALNTYVFLLREDKQFGDLVY
                     EWTASGAEPIGPKVQNLRMADFPWWREQLEGDGLLRITDVSQLSPTAWRERRVYEELG
                     IQSLLAQRLFLYSDLIGFVGFANLPNTSVLHKSDQVLLE"
BASE COUNT         1408 a         2792 c         2541 g         1373 t
ORIGIN      
        1 gtcggcgaac aactggagca ggccgtcgac accaccgcgc gctatgtcgg ccagcagacc
       61 gaaaccctgg cccaacacat cgacgcggaa ctgaacgccc tgcgcgcccg gctcaacgaa
      121 tatgaagcgc gccatgccgg cgacgacgat gccgcgcgcc gcgaatggca atccacccgc
      181 gccggcctgg aagcccgcat gaatcgcctg gatgaagagg ccggccggtt ggcggaccag
      241 gccgacgaca aagtcgaagc ggcccggcgc gacctcgagg ccggcatggc cgacctgcgc
      301 cgcgcgattg atgacctgga aaactgatcc cccggagccg ccccccacgc cccttcgatc
      361 gattgacggg aggaaccacg atgcgctcac gccttcatgc cgcggccgcg ctgctgttgc
      421 tggcaatatc gccgctcgtc gtgacgagct gctacggacg tttcccgctg acgcacgccg
      481 tttatgattt taacggcgaa atcggcaacg acgccggcaa cgccgagaaa tgggtcgaat
      541 cggccctctt ctgggtcttc gtcatcctgc cggtctacca actggcctcg ctcggtgatg
      601 cgattatttt caatgtcatc gagttctgga ccggcgatac gctcaggatc ggcgccgctc
      661 cgggccatgg cgcgctggcc ggcgtcgccg atcccgatgg cgcggcacgc cttgccgccg
      721 cgcctcagcc cggcccgtaa gtccgctgct gatccctacc gcttaggaac cggctttcgc
      781 catccgcaac tgctcgggat ccagcgcgcc tttgccgaaa atcaccggga acgattcgcc
      841 cttcgtcgtt cgcgtgcggg catccatgta acacacactg aatccgcgcc gggactgatc
      901 cgtcccgttc acgtccgacc ggtgcagcag caggttgtgg agcagaacgc attcgccggg
      961 gtccagttcc agatagataa tcctgtcctc ggggcaatgc tcccgttcct gctcgggcgt
     1021 gatcgtgtgg ccgaagtcgc tcagcagacc cagccggtgg gttcccggga tcacctgcac
     1081 gcagccgttg gcgatggtcg ccgcgtccag cgccgtccag agcgtcacca gcgggtcgcg
     1141 gtccagttcc cactgctcgc cgccgtcctg gtgccagggc agcaccgtgc cgcgccgtga
     1201 aggtttgttc atgaacatcg cgcggtaaac ggagatcgag gcatgggccc cgtagacttc
     1261 ggcgcagatc tcgcggaaga tcggcttctg catgaaccgc aggaagaccg gatccatctc
     1321 cagattctga attttccggt agttgagcgt cggccccttg aacccgagga cctgcgccgg
     1381 cgcatcttca tagcggcccg tctcgctgtc gagctgcatc agcatgcggt cgtaatcgac
     1441 gtcggcctcg cccagcatga tctgatcgat gcgcgtctga agcgccttga gctcggcgtc
     1501 gtcgaggaca cgccccagcc ggagataacc ctgctggaaa aattgaaacc actgttcgtc
     1561 ggaaaggatg cgattcatcg agggcaaccc ttggcctggg atgagcgctg gcggattgga
     1621 gcaaggtgtc agcgcgaata ggatccagca taccgcgaag cgaagccgaa cgccagatga
     1681 aagcgcttaa catgggcttt ttttgacttc cgccctcacc ccagccggcg tcaccccaat
     1741 ccccaatctc tcggagctaa atcgttaccg gccttccctt caaatggaaa tcggccgctc
     1801 ccacatactc cacgtccagc cattccccca ccgcccgcac gcccggcagc acaaccggtc
     1861 gccaggcgcc gttgtgcgcc agcgtccgcc ggcccgcggg atcactctcg aacgtcagca
     1921 cccgctcgcg tcgcccgacc agccgctcgt gccagcggcg cgtcgtcacc cgcaccgcga
     1981 cattcagccg gctggatcgt tccgtcacca ccgcgccggg cagaccggtc aggcgcgcgg
     2041 ccgccgtccc cggccgggcg ctgaaccgcg accggttcac cgcggcgaac ggcacccgct
     2101 cgaccagcgc caaggtctcg gcgaaatccg cttccgtttc ggtcgggaaa ccgacgatca
     2161 agtcggtcat caacgccagg tccgggtgcc gcgccagcat cgcctcgcag acggcctcaa
     2221 actcggccac cgtattctcc cgccccatcg cccgcaggac gccgtcggca cccgactgca
     2281 ctggaatgtg caagaaccgg aacaggcgcg gatgatccaa cagatcgagc caggcttcca
     2341 gttccgcact cacccactgc gggctggtca tccccagccg gatgcggaaa tcgcccggca
     2401 attcggcgat gcgcccgacg agccccgtca acgtgtaacc cgcctcgcgg ccgtacgccc
     2461 ccacgtcctg cgacgtgatc cagatttcac gcacgccttc gtcgatcccg cggcgcgctt
     2521 tctccaccag atcgctcggc cggaacgagc gcaaccgccc ccgcgcaagc cgggtctggc
     2581 aaaagctgca tgcgctgagg cagccgctcg cgatcggcag gatttcaacc agcggattgc
     2641 gccgcgcggt cggcagtcgt tcgcgcggtg cgccgcgctg ccgctccccg cgcagcacat
     2701 gcggcgcgcc cccgcgcatc gcctgttcca ccacctcgtc cacgcgcccg atcgcgtcgg
     2761 gccccagcgc cgccaccccg gccagcaggt cgctccgctc gtaggccttg gggatgcacc
     2821 ccgccacgac cagccgcggc ccctcgcccg ccagcgccgc ggccttcagg cgtcgaaacc
     2881 gcttctcgaa attcagatac gtgcgatcct tgaccgtgca ggtgttgacg atgatcacgt
     2941 cggccgccga aggatcgctt acgacgcgat ggccggcccg ctgcagcacg ccggccatgg
     3001 cttcgccgtc ggctgcattg aacgcgcagc cgtaggtctc gatgaaaacc gcatggccgc
     3061 cggcctcggc ggcgggatcc atggcatcgc gggaagtctc ggcggcggga atcatcctgg
     3121 tggcgtccat tggggtcgtc cgcgcgcggc ggcgcgcagc cgtccattct aagctcccat
     3181 tcaccggcct tcaacggctt gcaccagaat tcggctcccg gctcgcgtcc cccgtccccg
     3241 gctcttcaaa tcccccttcc ccgcaatcct cggatacgac gcgtgtagtt atattgtctg
     3301 ggcttcgtcc caaacatctt cttaattgcc ttaaatcttt cagtgtggtc cgaaaaaccc
     3361 atttgacgac acgcgtagta tagcttacca tccccggtca tctgatggga atatcccaaa
     3421 tcctcgcctc agcggcatac ttcttcatag gaaaggttgt tccaccatgc gtcttgccgt
     3481 ccgacttccg gtcgcgctgt tgcttctggc actgctggcc ggctgtagct ggctgcgtca
     3541 ccgtcccacg gacacgccat ccctcgcctc gcccgatgaa ctgcgccggg cgtacagcct
     3601 cgtctcggac ccatcccgct attacgacaa ggtccacggt gcctggctcg ggaagctgat
     3661 cggtctggcc ctggcccagc cagtcgaggg ctggtacaag gagggcatcc gcgaacgcgc
     3721 cgaagaggtc ggcgcctatc cggtccaggg ctatttcccc agcaatttcc gagggattcc
     3781 cggctgcgaa ccgtggctct acggcaacct ggccggctcg ccttccaacg acgacgtcga
     3841 acagatgctg ctcgccctgg tgaccctgcg cgagcgcggg gtcgatccga cgccgcgcga
     3901 tatcgccgaa atctgggata aatatctccc ctatggcaac gtcgcttccg gcgtcgcgct
     3961 ctataatatc cgccgcggca tctggccgcc cgaatccgcc accaccgaca acccgtatca
     4021 gaaccggatc ggcggccaca tgcgcgtcga catctggggc atggtcgccc ccggctgccc
     4081 ggcgctcgcg gccgatctcg ccgagcgcga cgccagcctc gcccacaccg gcgaggggat
     4141 ctacggcgcc cgcttcgtcg ccgccgccgt cagtctggcg atgttcgagc gcgaccccaa
     4201 aacgattctg acccaggccc tggagttcat ccccgccgat tgcgaatacg ccacctacgt
     4261 gcgcgacgtc atcggctggc acgcccagta ccccgactgg gaggacgcct atgcggagct
     4321 ggaccgcaag tacggcttct accccgacgg cacgcgcgag cgcgagtacg acgacccccg
     4381 attcgaccgc tccattccgc gttatcagtg gaacgatgcc cggctgggcc tggcgaccct
     4441 caacggcgcg gcctgcgccc tggccctcct ctacggcgag ggcgacttcg ccaggaccat
     4501 ctgcctgtcg acgatgatcg gctacgacac cgactgcaac gccggcaccg tcggcgccat
     4561 cgtcggcgcc tccattggcg ccagccagat ccccccctac tggatcgagc cgctgcagga
     4621 tacttaccgg tcccacgtca agccgatcgg caaggaggcc agcatcagcg ccatcgcgcg
     4681 cgaaacggcg ctctacggct atcaggtgat cgcctcgcgc ctcatcgagc acccgtgaag
     4741 ctgcaacccc gctgctgatg cctggcaggc cttcgcgctg gtccagcccc gccagccgaa
     4801 acccgaatga tatccgtcgg atcaaccggt ccgacggatt tccttttcct cccgtcgtat
     4861 cccgcatctg ctcgcgccaa agctcatgct ggtgcattgc gcatgaacca tgggtgcagg
     4921 ccgagagaga aaatggtcgg gtagtggttt gagacgcggt acgaacacct aggacggatc
     4981 agacgggggg acgtgtccca taacggcgac agggcttcgg caacgcgaac ggggccgata
     5041 agaaataagc agcgggtccg tgatgccggg acgcggggtt gccccgcgag gtgaaatccg
     5101 gcgcgggcct ccggccgacg ccggccggtc gaggccacca tgatcccaga acccgccccc
     5161 gatgcggaga agccatgatc gccacggata tcacagatcc aaccaccgcg gcggcggaac
     5221 ccggcttcaa cccgccggcc gacccggcaa cgaccgcggc gaacgaaacc gcggtgctct
     5281 tcgtcgacga cgacgccaat gtgctggccg cgtatcggcg catgttgtcg cagcgcttcc
     5341 ggctggagac ggccgcctcg ggggccgagg gtttgcagcg gctgcgcgag gggggaccct
     5401 tcgccgtcgt cgtctcggac ctgcagatgc ccgcgatgaa cggtatccag ttcctcagtc
     5461 gcgcgcgcga ggtttcgccc gacacggtgc ggatgatgct gaccggccag gcggacctga
     5521 gtcgcgcgat cgatgcggtc aacgagggga acctcttccg gtttctgacg aagccgtgcc
     5581 cggcggacat gctggccaag gcggtggagg cggggctgcg gcagtatcaa ctggcccgcg
     5641 ccgagaagga tctcctgcag gagacgctgc gcggcgcggt caaggtgctg gtcgatgtcc
     5701 tctcgctggt caacccgccg gcgttcgggc gcgcggcgcg ggtgcgccgg ctggcgcgtc
     5761 aggtggccga ggaactgcgc ctcgaggacg catggcaggt cgaaatcgcg gcgatgctct
     5821 cgcaactggg ctgcgtcacg gtgcccgaaa aaacgctgca caagcactgc ctggggcagg
     5881 acctgacgcc cgaggagtgg cgcatggtgc ggcgccaggc gcggatcggc gccgatctgc
     5941 tgcgctgcat cccgcggctc gaagcgatcg ccgagatgat cgcgcggcag gatgtgccgt
     6001 ggcgcgagca gcgcgagcgc ggcgcggccg gcgaggcgcc cggcggcgcc gatgtgctga
     6061 aggccgccgt cgatctggat acgctgatct cctccggacg ccatttcgct caggcgctgg
     6121 cggcgctgcg cgggcgccgc ggcgaatacc atccggccgt actgacggcg atcgaacggg
     6181 tgcagaagct ggatgccggc tatcagatcc ggcaggtggc gctcgatgaa ctccggccgg
     6241 gaatgatcct ggcgcaggac gtggcgacgg gcggcggcat gctgctgctc gcgcgcggtc
     6301 aggaggcgac cgaatcggtc tgccagcggc tgtgcaacat ggcacggatc tacgaggggc
     6361 tacccacggc ggtgaaggtg atggtgccgc gcggtcagga caatgagggc tcatagcctg
     6421 gattgctcca ggaagcattt gagctgacga acctcgagtg ccgagaaccg gaataagcga
     6481 gagccattgg tgtggtggag gcggggcgcg gaagcatgga atagggcggc ttctccgcgc
     6541 ccttctcttt tgtcccagtt ttctctcaca tccattccct cactgtttca gaaatcgatc
     6601 acttcgtggc cgccgtgttt cctctggcga acaccgggtg cgggcggctg gaatgacctg
     6661 ggacgacccg tcaggaaggc aatgtcccct tccggggaca ttcaagggtc cggtcttatc
     6721 ggccgctgcc ggtgtgccgt ttaaaattta tggcgatggt ctcgcagggg cagcggccgc
     6781 cgtccgttct catcaagcgc ttcatgctgg ggtagttcgc gatgctttcg gatctcgatt
     6841 ttcagcaagt cgaatggaca ccggcggctc cacccgaaca gggcgaaagc gacaccctcc
     6901 tgctgactga ttccgatccg gaacggggcc ggcggatcgc cgaccgcttc gcccggcagg
     6961 gctatcgctg tcagctggcg gtggatcatc ccgacgcact gcgcaagctg attgccgagg
     7021 agacggcgct tgtgctgctg gtctgcgatg ggtcggccgc ggagatctac ccgtggctcg
     7081 acgccatcgg ccaggcgcgc aacgcgccgg tgcagctggt gctcgtcccc gaggaacgcg
     7141 acgcccagcg agtcgttatc caggcggacg cgtttgatta tctcgtgctc agccgtctgg
     7201 acgaccaggg ctactgggag ccgatcctgc aacgcgcgct gcgccagcat cacgccaatc
     7261 gcgaagcctt cgcggtccgc cgcatcctgc gccacaacac ggtgatcttc gacgcatcgc
     7321 ccgacatgat cctcgtattg gacggcaatc gccgcatcct gcactgcaac ctggcggtgg
     7381 agaactacac agggcgcgcg cggcgggagc tgatcggcga gcgcgcgcac caggtgcttt
     7441 gcggcgacca tcatccggcc gaggcttgcc cgttgggcgg cgggttgcac gagggagacc
     7501 gcacgcgccc gatctgtcgc atgcagcggc gaacgcaaac cttccagatg cgttcaatcc
     7561 ccctgtttac gcgcgacggc aaggtctggg ggacgatgga gatcctccag aacgtcacgg
     7621 aactgatcca ggccgagcag gcgctcaatc accggcttga aattgagaag ctcatcgccg
     7681 aaatctcggt tgagttcgcc cataacgagg acatggacca ggcaatccac cgcacgctgg
     7741 cgcgcatcgg cctctggagt caggccctca acacctatgt cttcctgttg cgcgaggata
     7801 agcagttcgg cgatctggtc tatgaatgga cggcgtcggg ggccgagccg atcgggccga
     7861 aagtccagaa cctgcgcatg gcggattttc cctggtggcg tgaacaactg gagggcgatg
     7921 gactcctgcg gatcaccgac gtttcgcaac tgtccccgac ggcatggcgc gaacgacggg
     7981 tttacgagga actggggatc caatcgctcc tcgcgcagcg cctgtttctg tactccgatc
     8041 tgatcggatt cgtcggtttc gccaatctgc ccaacacgag cgtgctgcat aagagcgacc
     8101 aggtgctgtt ggaa
//
LOCUS       DAOPCO010000178         8110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8164_length_8110_cov_4.481937, whole genome shotgun sequence.
ACCESSION   DAOPCO010000178 DAOPCO010000000
VERSION     DAOPCO010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8164_length_8110_cov_4.481937"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..836)
                     /locus_tag="PLS90_11895"
     CDS             complement(321..836)
                     /locus_tag="PLS90_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK03150.1"
                     /translation="MQNYLGEIIYATRLKLNLTQTEYGQQFNVSGPAIFKFEKGYVRP
                     SLKLWLDMARQAGISERRAVLIWVKRSLPERYRDYIGLEQAYESARRAVGRQKDYSRF
                     TERAELLREMEQDPAMPEALRQFVRDDENWALYKPTGEEINKLRDVFGPLGAGTRELY
                     RDALRVVREFV"
     gene            complement(1306..1381)
                     /locus_tag="PLS90_11900"
     tRNA            complement(1306..1381)
                     /locus_tag="PLS90_11900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1345..1347),aa:Glu,seq:ctc)
     gene            complement(1420..1495)
                     /locus_tag="PLS90_11905"
     tRNA            complement(1420..1495)
                     /locus_tag="PLS90_11905"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1460..1462),aa:Gln,seq:ctg)
     gene            complement(1740..2345)
                     /locus_tag="PLS90_11910"
     CDS             complement(1740..2345)
                     /locus_tag="PLS90_11910"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPK03151.1"
                     /translation="MAYELPKLPYPYDALEPHIDAQTMEIHHTKHHATYVTKTNAALE
                     AHPDLAKKDVEDLLRGLNQVPEGIRTAVRNNGGGILNHNLFWQIMSPNGGGEPTGDLA
                     DAIKSAFGDFEKFKEEFANAAANRFGSGWAWLVNDGGKLSITSTPNQDNPVMDGKPLP
                     ILGLDVWEHAYYLKYQNRRPDYIKAFWNVVNWDEVARRFKG"
     gene            complement(2716..3114)
                     /locus_tag="PLS90_11915"
     CDS             complement(2716..3114)
                     /locus_tag="PLS90_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HPK03152.1"
                     /translation="MPSPASRIAREAKTIQVMIAMFCREHHACAGAARPGRSAPLCAG
                     CRELRDYALRRLERCPFQAGKTTCALCPIHCYRADMRERVRAVMRWAGPRMITRHPLL
                     ALGHLLDQRRKTPVRAAGGGRAASSSAAGA"
     gene            3287..5260
                     /locus_tag="PLS90_11920"
     CDS             3287..5260
                     /locus_tag="PLS90_11920"
                     /inference="COORDINATES: protein motif:HMM:NF018471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HPK03153.1"
                     /translation="MPDDPATQPPRRPVMVYDGQCGFCRYSVQLIRHWTRERIVYRPF
                     QEVAERCKSVGADPAEARRRLAEAVHLIEPETGAATADAPAAGTRDNAPLVGRTTRGA
                     EAILRAYAIGGLRWPHAVYRRVPGVRPAGERLYAWIARHRPLVDRLARGLEGRRPVPR
                     SWRLTRWMVLRGVALIFFIAFASLLVQVRGLIGSDGILPVGEYLERASGMLGPGAWRA
                     VPTLSWISPGDAMLVGQCLAGMALAILAGLWIAPGPALLGCWGLYLSLTVAGQRFMGY
                     QWDALLLETGLLAALWAPWRRRPRAEDEPAPSAIGRWLLWWLLFRLMFESGVVKLASG
                     DPLWRELAALDYHYWTQPLPTWIGLLAAHLPAWFQHLSCAVMFGIELGAPWLILFGRK
                     GRLVAFALLVALQVLILLTGNYTFFNGLTIVLCLSLLDDAAWVRFVPRAKRARALLRG
                     CRQWRRPAPRGQRGALAVVAGIAIIVGGGQVVRAFDRGLAPRGLVGWVETRVAPLNSF
                     NPYGLFAVMTPSRPEIVIEGTADGVAWTPYEFKWKPGRLDRRPRFVAPHQPRLDWQMW
                     FAALGSLRGNAWIVTLMERLLEGEPSVLGLLGENPFKAAPPLAVRAVLYEYRPADPAV
                     RRETGQWWTREPMGLYAPPLALRVDRAILADSE"
     gene            5424..6308
                     /locus_tag="PLS90_11925"
     CDS             5424..6308
                     /locus_tag="PLS90_11925"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPK03154.1"
                     /translation="MTRLEHPYQHLDPAGGWLRGNLHTHTTQSDGVEAPQAVIDAYAA
                     AGYDFLMLSDHDIFSGAELYRTLDARGMTLIPGNEVTANALHLLHVNGDRRVEPHRNR
                     QQVIDEIAATQGFAIVNHPNWQWGPTPLCPPEELAAMSAFAGMEIFNGVIRRLEGSPY
                     ATDCWDKRLSQGERMWGYAHDDSHEPGDRFLGWNMVWSADRGVDALVEALRHGRFYAS
                     TGVVIRKLESDGMQVHIEAENADRIVAVTDFRLRLGQSDTNDLTVTVPETKQYVRFEC
                     WGRGEEFAWSQPFFVVKD"
     gene            6640..7689
                     /locus_tag="PLS90_11930"
     CDS             6640..7689
                     /locus_tag="PLS90_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03155.1"
                     /translation="MGRMGCNWAFAAILCLWAAGATPAWASIYLDEDFEGVLFVDRDW
                     PVRSADEPEPTEASVALMGVNLRVGGDSDGSRQPKVQVVNEGAVSELRSFHGARSLAL
                     NNGSISVGPDGHPALNKSTQAFRVLQFALSVDQDSLAVADGTIVGRIQQDWSTNGNLN
                     TVDYSLVLNLVKNDADDRIDLICQNNDALLGRLQGIRGDWLVVTIVNNIGTMEATYEV
                     EQTVFMSHVDPFTDEVRCAEMVLPTGIHIFASSQTPMTYLTYEEINAAQTGGAGSRWG
                     NSNLDTDQTAELGWSIASLAGTDIFIDNLYWDCGYHSNPSRAQEMEQAARMTTFDDPG
                     TESAISGVRSWSFYE"
     gene            7804..>8110
                     /locus_tag="PLS90_11935"
     CDS             7804..>8110
                     /locus_tag="PLS90_11935"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPK03156.1"
                     /translation="MARRTAQPEPISQTSDGLTIPMEVQALREPAPEGPHLVELSGEH
                     RGRIHALDAPATTIGRKRGLPIRLVEAGVSKEHCSLLRAGSAVSIVDHHSTNGTLLNG
                     "
BASE COUNT         1375 a         2560 c         2784 g         1391 t
ORIGIN      
        1 aatggtaaag gcaagtcgga cttaagggag gaaaggtgtc aagcgatagc ttaccccgcc
       61 gccagggacg cgcgggccgg ggttcgcgcg cgaaaagggg gtggtgggcg tggttacccc
      121 caagacacca tttggtccaa aaaagtggag aagttccctt gtcccgtgaa catccgcatc
      181 gccagacact cgtgccgcac gaggcaaaag gaactcccca ccatgagcac ctggccacag
      241 cccgaatcgt tttcgacaac agcggcgggg ggccgctgcc gggcggcggc cgcggggatt
      301 acacggctcc ctcgcgcggg ttacacgaac tcgcgcacga cgcgcaacgc atcgcgatag
      361 agttccctgg tgccggcgcc gagcggtccg aagacatcgc gcagcttgtt gatctcttcg
      421 ccagtgggct tgtagagcgc ccagttctcg tcatcgcgga cgaactggcg cagcgcctcg
      481 ggcatggccg ggtcctgctc catctcgcgc agcagctcgg cgcgctcggt gaagcgcgag
      541 tagtccttct ggcggccgac ggcgcggcgg gcgctctcgt aggcctgctc caggccgatg
      601 taatcgcggt agcgttcggg caggctgcgc ttgacccaga tcagcacggc gcgccgctcg
      661 ctgatccccg cctggcgggc catgtcgagc cacagcttga gcgacggccg gacgtagccc
      721 ttctcgaact tgaagatcgc cggtccggag acgttgaact gctggccgta ttcggtctgg
      781 gtcagattga gtttgagccg cgttgcgtaa ataatctcac caaggtaatt ttgcattatc
      841 aacctcccag tagccgtgat gcggggatga taacaaccgg cgcggcggct gtcgaccgtt
      901 gactgcgcgc gcgggacgtg tcgggttacg ctttaagcac cccctgacaa cgcaaggtcg
      961 cggaccgcaa ccggactggc gaccgggagg aaaacgacat gctctgccaa gcttgatgga
     1021 cagcactaca caacttatgt catcagacaa ctgaaacatg aagaaaggat tgatcagaca
     1081 gagaagccct ccacgcgaga gtcgaggaat tcatgaatcc cgatttctaa gagggatcga
     1141 acgtgcgtga tctgcgagcg aggaatgcca cacgcgaacg gggatgacgt ctcggaacgg
     1201 gcgcggcagg aaatcaccca tcaacgctcg actccaccga tttaggacca aatttttgga
     1261 gaacaaaaag gaggcagcgc gctggcccgc gcggcgggga aggcgtggta gcgccaaggg
     1321 gaatcgaacc cctgtttccg gcgtgagagg ccggcgtcct ggaccgctag acgatggcgc
     1381 cacggaaggc ggggccgggg aaggccccgt gaaaaggaat ggctggggag gtaggactcg
     1441 aacccacgat cttctgggcc agaaccagac gtgttgccag ctacaccact ccccagcaac
     1501 atccgccctt cttttacatg gtggagcccg ccgctgtcaa gcggctgatc agcgcaaaca
     1561 gggaaggctg cgagcgcccg cggcgggatc acaaccccca tcaagccccg tcggcgcgaa
     1621 tcgtatgccc gcggccgtgg cgcgtcgctc ccaccggcgg cctcgcatcc aaagaaacgg
     1681 gggcggccgg agccgccccc gttccatacc aggccgcttg tcgcggcgcc gtcgcgggct
     1741 cagcccttga agcgccgggc gacttcgtcc cagttgacga cattccagaa cgccttgatg
     1801 tagtccggcc ggcggttctg atacttcaga tagtaggcat gttcccagac atcgaggccg
     1861 aggatcggca gcggtttgcc gtccatcacg gggttgtcct ggttgggggt gctggtgatc
     1921 gagagcttgc cgccgtcgtt gaccagccag gcccagccgc tgccgaagcg attggcggcg
     1981 gcgttggcga attcctcctt gaatttctcg aagtcgccga aggcggactt gatcgcgtcg
     2041 gccagatcgc cggtcggctc gccgccgccg ttgggcgaca tgatctgcca gaagaggttg
     2101 tgattgagga tgccgccgcc gttgttgcgg acggccgtgc ggatgccctc ggggacctga
     2161 ttgaggcccc gcagcaggtc ctcgacgtcc ttcttcgcca ggtcggggtg cgcttcgagc
     2221 gccgcgttgg tcttggtcac gtaggtggcg tggtgcttgg tgtggtgaat ctccatcgtc
     2281 tgggcgtcga tgtgcggctc gagcgcgtca tacggatacg gcagtttggg cagttcgtaa
     2341 gccatggtga acggtctcct tcgctcaatc gatcgtgccg ggcggggcgt cgggggattt
     2401 tcccgctcgg cgggccatcg gcatgctggg aggccagccg tgcaatccgg aaggctggcg
     2461 accggggaaa acgcgaatgt tcaagcaagc aacggatgcg ccatgcgtcg aactgacgcg
     2521 caaccggcgc ccgtcgggcc aaccggttgt ccgcaatccg tacgcatgcg ttaccgaaca
     2581 ttttacgctt tgagcaggac aagtcaagcc gcggcaagcc gttgaacccg ttaagttcgt
     2641 tggcgcttgt tgttcaggaa gaatttcgcg ggacaatcac gaggacaaag acgaggacag
     2701 ggaagaaatc cagcgtcacg cgccggcggc gctggatgag gcggcacgac cgcccccggc
     2761 cgcgcgcacg ggcgtcttgc gccgctgatc aagcaggtgg ccgagggcca gcagcgggtg
     2821 gcgcgtgatc atccgcgggc cggcccagcg catcacggcg cggacccgct cgcgcatgtc
     2881 ggcccggtag cagtggatcg ggcagagcgc gcaagtcgtc ttgcccgctt gaaacggaca
     2941 gcgttcgaga cgccgcaggg catagtcgcg cagctcgcgg cagccggcgc agagcggcgc
     3001 ggaccgtccc ggccgggcgg cgcctgcaca ggcgtgatgc tcgcggcaga acatggcgat
     3061 catcacctgg atcgtcttgg cttcgcgggc gatgcgggat gcgggggatg gcatgggcag
     3121 ggccgaaaga agatctgggg atcataatat ctgtatcatc tcgagccgtc aactcctgaa
     3181 cttgcgccgg acaacgccgt gcctgatttt cccgcgcctc cgcacaagac ggcgccgccc
     3241 gcttgcgcta aactgaccga ctcactcccc cttcaggaac cggccgttgc ccgacgaccc
     3301 cgccacccaa ccgccgcgcc gccccgtcat ggtttatgac ggccagtgcg gtttctgccg
     3361 ttactcggtc cagctgatcc ggcactggac ccgcgagcgg atcgtttacc ggccgttcca
     3421 ggaggtggcg gagcgctgca agtcggttgg cgcagacccc gcggaggcgc gccgccggct
     3481 ggccgaggcc gtccatctga tcgagccgga aaccggcgcc gcaacggccg acgcgcccgc
     3541 cgccggcacc cgcgacaacg cgccgctggt cggccggacc acccgcggcg ccgaagcgat
     3601 cctgcgcgcc tatgccatcg gcggcctgcg ctggccccac gccgtttatc gtcgcgtgcc
     3661 cggcgtgcgg cccgccggtg agcggctcta cgcctggatc gcccggcacc gcccgctggt
     3721 ggaccggctg gcgcgcggcc ttgagggccg gcgcccggtc cccaggagct ggcggctgac
     3781 gcgctggatg gtcctgcgcg gcgtggcgct catctttttc atcgcgttcg cctccctgct
     3841 ggttcaggtg cgcgggctga tcggatccga tggtattctg cccgtcggcg agtatctcga
     3901 gcgggcaagc ggaatgctgg ggccgggggc gtggcgcgcc gtcccgacgc tgtcgtggat
     3961 cagcccgggc gatgcgatgc ttgtcggtca gtgcctggcg gggatggcgc tggcgattct
     4021 ggccggcttg tggatcgcgc ccgggccggc gctgctcggc tgctggggac tctatctctc
     4081 gctcacggtc gccggccagc ggttcatggg ctaccagtgg gacgcgctgt tgctggaaac
     4141 gggcctgctg gcggcgctgt gggccccctg gcggcggcgg ccgcgcgcgg aggatgaacc
     4201 ggcgccgtcc gcgattggcc gctggctgct gtggtggctc cttttccggc tgatgttcga
     4261 gtcgggggtg gtcaagctgg ccagcggcga cccgctctgg cgcgagctgg ccgcgctcga
     4321 ttaccactac tggacgcagc cgctgccgac gtggatcggc ctgctggcgg cgcacctgcc
     4381 ggcatggttc cagcacctgt cgtgcgccgt catgttcggc atcgaactgg gagcgccgtg
     4441 gctgatcctg ttcggccgaa aaggccggct ggtggctttc gccctccttg tcgcgctgca
     4501 ggtgctgatc ctgctgaccg gcaactatac attcttcaac gggttgacga tcgttttgtg
     4561 cctgtcgctg ctggatgacg cggcgtgggt gcggttcgtg ccgcgggcga agcgggcgcg
     4621 ggcgttgctg cgcggctgcc gccagtggcg ccgccccgcc ccgcgcgggc aacggggcgc
     4681 gctggcggtc gtggccggca tcgccatcat cgtggggggc ggccaagtcg ttcgcgcatt
     4741 cgaccgcggt ctggcgccgc gggggctggt cggttgggtc gagactcggg tggcgccgct
     4801 caacagcttc aacccctacg gtctgttcgc cgtcatgacc ccctcgcggc cggagatcgt
     4861 gattgaaggg acggccgacg gggtggcgtg gacgccgtat gagttcaagt ggaagcccgg
     4921 ccggctggat cgccgcccgc ggttcgtcgc gccgcaccag ccccggctgg actggcagat
     4981 gtggttcgcc gcgctgggct cgctgcgcgg caacgcctgg atcgtgacgc tgatggagcg
     5041 gctgctggag ggcgaaccgt cggtgctggg gctgctgggg gagaatccgt tcaaggcggc
     5101 gccgccgctg gccgtgcgcg cggtgctcta cgaataccgc ccggccgacc ccgccgtgcg
     5161 gcgcgagacc ggccagtggt ggacgcgcga accgatgggt ctgtatgcgc cgccgctggc
     5221 gctgcgggtc gatcgagcca tcctcgcgga ttcggaatga gggtgctctt agcgacaagt
     5281 ggggttcgcc ccagcagaac ttccccatgc tcggcggcgc ggcgcgacgg cccgttttcg
     5341 ccggccgatt tccccttgca ttcgttccct gtcaacgcaa gattggcaag gctgacccat
     5401 tctatcggag gcatccatcg cccatgaccc gactcgagca tccctaccag cacctggacc
     5461 cggccggcgg gtggctgcgc ggcaatctgc atacccacac gacccagagt gatggcgtgg
     5521 aggcccccca ggccgtgatc gatgcctacg ccgcggccgg ctacgatttt ctgatgcttt
     5581 cggatcacga cattttttcc ggcgcggaac tctaccggac gctcgacgcg cgcggcatga
     5641 cgctcatccc gggaaatgag gtcacggcca acgcattgca cctgctgcac gtgaacggag
     5701 accgccgggt cgaaccccat cgcaatcggc aacaggtgat cgatgaaatc gccgcgaccc
     5761 agggcttcgc gatcgtcaac catcccaatt ggcagtgggg tccgacgccg ctctgcccgc
     5821 cggaggagtt ggcggcgatg agcgcctttg ccggcatgga gatcttcaac ggggtcatcc
     5881 gccggctgga ggggagcccc tacgcgaccg actgctggga caagcggctc tcccagggcg
     5941 agcggatgtg gggctacgcg catgacgaca gccacgagcc gggcgaccgg ttccttggct
     6001 ggaacatggt ctggtcggcc gaccgcgggg tggacgcgct ggttgaggca ctgcggcacg
     6061 gccggttcta tgcgtcgacg ggggtggtca tccgcaagct ggaaagcgac gggatgcagg
     6121 tccacatcga agcggaaaac gccgaccgga tcgtcgccgt caccgatttc cggttgcgac
     6181 tgggccaatc cgacacgaac gacctgaccg tgaccgtccc cgagacgaag cagtatgtgc
     6241 ggtttgagtg ttggggtcgc ggcgaggaat tcgcctggag ccaacctttt ttcgtggtca
     6301 aggattgaat tcgcaatggt atataaagtg aggtagcgga caaaattcgg cagggtcttc
     6361 gggagcgagg acgtgagaca agggacggat ttttgtgtca gcgacaaagc atgcattcct
     6421 gaacgtttat gttgacaata tcgattggga tgctggacat gatatcctcg gctgagggcg
     6481 ggccgagggg atgacgacct tcaacgtcga gggcgcgaaa cgggaaggtc cagccgtccc
     6541 gccgaccgga gcaataggac tccgcgcttg attctcgcgg cgcgaccgcc ggaagcccca
     6601 cctttcgaac cttcccacga gcagaaggga ccccatccga tgggccgcat gggttgtaac
     6661 tgggcgtttg ccgccattct ctgcctctgg gcggctggtg cgaccccggc ttgggccagc
     6721 atctatctgg atgaggattt cgagggcgtt ctgttcgtcg accgcgactg gcccgtgcgg
     6781 agcgcggacg agccggagcc gaccgaagcc agcgtggcgc tgatgggcgt caatctgcgc
     6841 gtcggcgggg attcggacgg ttcccgccag cccaaagtcc aggtcgtcaa tgagggggcg
     6901 gtgtcggagc tccgttcctt tcacggcgcc agatccctgg cgctcaacaa cggctcgatt
     6961 tcggttggcc ccgacggcca tccggccctg aacaagtcga cccaggcctt ccgcgtgctg
     7021 caatttgccc tgtccgtcga tcaggactcg ctggccgtcg ccgacggcac gatcgtcggt
     7081 cgcatccagc aggactggtc gaccaacggc aacctgaaca cggtcgatta cagcctggtc
     7141 ctcaatctgg tcaagaacga cgccgacgat cggattgacc tgatctgcca gaacaacgac
     7201 gcgctcctgg gccgcctgca agggattcgg ggcgactggc tggtcgtgac gatcgtcaat
     7261 aacatcggca cgatggaggc gacctacgag gtggaacaga cggtgttcat gtcgcacgtc
     7321 gatccgttca ccgacgaggt gcggtgcgcg gagatggtcc tgcccacggg gattcacatc
     7381 ttcgccagtt cccagacgcc catgacctac ctgacctacg aggaaatcaa tgcggcgcag
     7441 accggcggcg cggggtcgcg gtggggaaac tcgaacctcg acaccgacca gacggcggaa
     7501 ctgggctggt cgattgcctc gctggccgga accgacattt tcatcgacaa cctgtattgg
     7561 gattgcggct atcacagcaa ccccagccgc gcgcaggaga tggaacaagc cgcccgcatg
     7621 acgacgtttg acgacccggg gaccgaatcc gccatcagcg gggtcagaag ctggtcgttc
     7681 tacgaatagt gggcgaggcc tggtcgaagg aagcggcgtg gcggatggcg cgggcgcgtt
     7741 tcgactgttg ccctcgaagt ccgcaatgta gaatcgaatc atcccccgcc gaatgggccc
     7801 cgcatggcca gacgcaccgc ccaacccgag ccgatatccc agacgagcga cggcctgacc
     7861 attccgatgg aggttcaggc gctgcgcgaa ccggcgccgg agggcccaca cctggtggag
     7921 ctcagcggcg agcatcgcgg gcgaatccac gcgctggacg cgcccgcgac gacgatcggc
     7981 cgcaagcgcg gcctgccgat ccggcttgtc gaggcggggg tctccaaaga gcattgttcg
     8041 ctgctgcgcg cgggctcagc ggtttcaatc gtcgatcatc actcgaccaa cggaacgctg
     8101 ctcaacggcc
//
LOCUS       DAOPCO010000179         8089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8193_length_8089_cov_6.638661, whole genome shotgun sequence.
ACCESSION   DAOPCO010000179 DAOPCO010000000
VERSION     DAOPCO010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8193_length_8089_cov_6.638661"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PLS90_11940"
     CDS             <1..268
                     /locus_tag="PLS90_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03157.1"
                     /translation="WRVLRPGGAFLIRDLYRPPDEARLEELVRLHAGGSNAHQQAMFR
                     DSLMAALTPAELRALLREIDLNDLELTVDTDRHMSLQRAARLPS"
     gene            327..578
                     /locus_tag="PLS90_11945"
     CDS             327..578
                     /locus_tag="PLS90_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019296069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HPK03158.1"
                     /translation="MTMDPVIAQCAPYVVEEEPGTRYWCRCGKSARQPYCDGSHKDTD
                     FQPIPIKFEKKERVAWCGCKHSLKQPFCDGSHRNLMKKE"
     gene            complement(646..1029)
                     /locus_tag="PLS90_11950"
     CDS             complement(646..1029)
                     /locus_tag="PLS90_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03159.1"
                     /translation="MAMTWHHFKQAAIAFHVVWVVIVVAILGLLAGSLDLLDRNGQII
                     FAANFCAGALIGAGLYFYRVYFGLYYSIVFIAVWATAWCVLLLVSGRASVGAMVVIPA
                     AALAWIAVVLAAFKRDQMTRRTELR"
     gene            complement(1089..1703)
                     /locus_tag="PLS90_11955"
     CDS             complement(1089..1703)
                     /locus_tag="PLS90_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK03160.1"
                     /translation="MAQIEWLGHASFRLRNGATVYIDPWKLKADEPKADVILVSHTHY
                     DHLSIEDIEKIRTPQTRILGSMDCADKIAPPLIGARPGEAHTLAGVAVRTLPAYNINK
                     EFHPRANDWLGFIITLGGETIYYVGDSDLIPEMADAGRVDTALIPVGGTYTMTAAEGA
                     QAAERIGARRVIPYHWGDIVGTRADAEAFVRACPSTAEILEISR"
     gene            complement(2008..4803)
                     /gene="ppdK"
                     /locus_tag="PLS90_11960"
     CDS             complement(2008..4803)
                     /gene="ppdK"
                     /locus_tag="PLS90_11960"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPK03161.1"
                     /translation="MAKQYVFSFGRTKTDGRGDQRALLGGKGANLAEMTRIGLPVPPG
                     FTISTEVCTYFYENGRKYPAELKDQVAKSLARVEKEMGKKFGDAKDPLLVSVRSGARD
                     SMPGMMDTILNLGLNEKTTEAVAEKSGNPRFAWDCYRRFVQMYGDVVMGVQRRKDEHH
                     DPFEHAIETIKKENNAETDLDLTVENLQELVRRFKALIRERTGQAFPEDPEKQLWGAI
                     GAVFGSWNNDRAIVYRRKYGIPHDWGTAVNVQTMVYGNMGDTSATGVAFTRDPATGEK
                     VFYGEYLVNAQGEDVVAGVRTPKPIALLKEEMPKAHKELETVRRTLEQHFKDMQDFEF
                     TIEDGTLYMLQTRNGKRTGLAAVRIACEMVKEGLITEEMAVQRIPADSLTQVLAPVFD
                     RAARDKAVKANRLLAKGLPAGPGAATGKVVFNAEEAVAWANRGEKVVLVRIETSPEDL
                     RGMIAAEGILTARGGVSSHAALVARQMGKICVCGCGDLHIDYGRKQMTVAGRTIKEGD
                     WISIDGTLGEVYEGEIDTAPSEIIQVLIDKTLDPKQSQTYQNFDRLMKWADKYRRLDV
                     RTNADQPGQAANAVAFGAVGIGLCRTEHMFFEGDRIYSVRRMIMAENEEHRRAALEDL
                     LPHQREDFAGIFRAMKGLPVTIRTLDPPLHEFLPHEESAINETAAKLKMSPDDVRRRV
                     NALHEANPMLGHRGCRLGNVYPEITEMQVRAIFEAAAEVMAEGIKVKPEIMIPLVGYP
                     KELALQLDVVNRVAEEVRKATGKKINYLVGTMIEIPRAALVADEIAKYAEFFSFGTND
                     LTQTTLGMSRDDSGNFLPQYQELDIIPSNPFATIDRTGVGRLMKIAIEESRKTRPDIK
                     LGICGEHGGDPQTVMFCHEIGLHYVSCSPFRVPVARLAAAQAALAEAASRKPAKKSAK
                     GAKKAKKAAGKTAKKRK"
     gene            5056..5988
                     /locus_tag="PLS90_11965"
     CDS             5056..5988
                     /locus_tag="PLS90_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPK03162.1"
                     /translation="MVEAGQILDRMETVIVGKRPVLELVLAAMLAGGHVLIEDRPGVA
                     KTLLARTLARVFTLEFSRVQLTPDLLPSDLTGTGIWNEHERAFVFQPGPIFGQIVLAD
                     ELNRATPRTQAGLLEAMEERAVTVDGVRHPLPAPFFVIATQNPIEQQGVFPLPEAQLD
                     RFLIQVAMGYPGYEDEVRIVMDQAAGHPLDELKPVAGARDFAELAAAVQQVHTDDSVL
                     GYLVRLARATRERDDLLLGASPRASLALHHMARTLAAIRGERFLRPDHVKAVAPAVLR
                     HRLILTPQARLAGVAPDDIIEELLAEVEAPIYTG"
     gene            6068..7384
                     /locus_tag="PLS90_11970"
     CDS             6068..7384
                     /locus_tag="PLS90_11970"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPK03163.1"
                     /translation="MLRADPRIGGVFSSRNILLAGVGFLFAWLLLLPAAPAQAALAGF
                     LLAAGGWLWLTHRGQLQELRIERHHRPRVFENDPLTVTLRVVRGRGWPVQMLEIEDQF
                     GASLQIAQRHLVPGLHEGWEVLIHYPVVAERHRGLYLIGPVNVRAADPFGIFHEQRQI
                     ETLTRLTVYPRAQPLGEYTIPGPEAAPGPSLDVAARLGQGDEILTVREYRPGDPPARI
                     HWRTSARRGALHVMQLNRTVQAELAVYIDLSRRARYGLGGEATTELAITAATSILTRA
                     HEARHRVSLAYAQREPVFFPPGSGLAHLHLLLDRLAIVEPGGETDFWRHIVPRALELP
                     AGSRAVLIVTAGVMPFEAAREMVRRLVARQVGVDLVLIDEEGFIRIYKDQEVDLRRGQ
                     PGFAELVEALSQSGARVFPLRRGAAELPPHPVAAAARPDWSRRGAA"
     gene            7525..>8089
                     /locus_tag="PLS90_11975"
     CDS             7525..>8089
                     /locus_tag="PLS90_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03164.1"
                     /translation="MRTSSPPAIRLRREPFIERWGGYLMAICLHIMLFAFLMMQPIEP
                     IPDEVEPPDRVRIALRPTPTPVPPRRVEPPTEPEPTPVVVPFPEPRQMAAPEPPEPVL
                     PEPLSEIEETLAAVPPPAPQPAPREEEAPRLIALLEPTPEPRVIEPVETPTLTPTPTP
                     TPTPTPTPTPTPTPTPTPTPTPTPTPTP"
BASE COUNT         1220 a         2894 c         2530 g         1445 t
ORIGIN      
        1 gtggcgcgtg ctgcgccccg gcggcgcctt cctgatccgc gacctctacc gtccgcccga
       61 tgaggcgcgg ctggaggagc tcgtccggct ccacgcgggc ggttccaatg cgcatcagca
      121 ggcgatgttc cgcgacagcc tgatggcggc gctgacgccc gccgagctcc gcgcgctgct
      181 gcgcgaaatc gacctgaacg atctggagct gaccgtcgac acggaccggc acatgtcgct
      241 gcagcgggcg gcccgcttgc catcgtaggc cctgcccgct agaatgaccg gcgttcaggc
      301 gactccagcc atttccacga gagaggatga ccatggatcc cgtcatcgcc cagtgcgcgc
      361 cgtatgtcgt cgaggaggaa ccggggaccc gctactggtg ccgctgcggc aaatcggccc
      421 gccagcccta ctgcgacggc tcgcacaagg acaccgactt tcagccgatt ccgatcaagt
      481 tcgaaaagaa ggaacgcgtg gcctggtgcg gctgcaagca ttcgctcaag cagcccttct
      541 gcgacggttc tcaccgcaac ctgatgaaga aggaatgagg aggaaggggc gccccggaat
      601 tcggtcgcgg actgggcggt tgatcggtag cgccgccggc ggcggctatc tcagttcggt
      661 ccgacgcgtc atctggtcgc gcttgaacgc ggccagcacg acggcgatcc atgccagcgc
      721 cgcggccggg atgaccacca tcgcgccgac gctcgcccgg ccgctcacca gcagcagcac
      781 gcaccaggcg gtcgcccaca ccgcgatgaa cacgatggaa taatacagcc cgaaatagac
      841 gcgataaaag tagaggccgg cgccgatcag cgccccggcg cagaagttcg cggcgaaaat
      901 gatctggccg ttgcggtcga gcagatcgag cgagccggcc agcagcccca ggatcgccac
      961 cacgatcacg acccagacga catgaaacgc aatcgccgcc tgcttgaagt gatgccatgt
     1021 catcgccatg atggattgcc tccgcggatt gacatcgccc ccgaccggcg cgcggccccc
     1081 gcttgtcgct agcgcgaaat ctccaggatt tccgccgttg acgggcacgc ccggacgaac
     1141 gcctcggcat cggcccgtgt gccgacgata tcgccccaat ggtacgggat gacgcgccgc
     1201 gcgccgatcc gctcggccgc ctgggcccct tccgcggccg tcatcgtgta ggtcccgccg
     1261 acggggatca gggccgtatc gacccgcccg gcgtcggcca tctcgggaat caggtcgctg
     1321 tcccccacgt agtaaatcgt ctcgcccccg agggtgatga tgaaaccgag ccagtcgttg
     1381 gcgcgcgggt gaaattcctt gttgatgttg taagccggga gcgtgcgcac ggcgacgccg
     1441 gcgagcgtgt gcgcttcgcc ggggcgcgcg ccgatcaacg gcggcgcgat cttgtccgcg
     1501 caatccatcg agcccaggat ccgcgtctgg ggcgtccgga tcttttcaat gtcctcgatc
     1561 gacagatggt cgtagtgggt gtgcgagacg aggatcacgt cggccttggg ctcgtcggct
     1621 ttcagtttcc acgggtcgat atagaccgtc gcgccgttgc gcagccggaa ggatgcgtgg
     1681 ccaagccatt cgatttgagc catagagcgg ttctcctttt cttgtgcgtg cgtcagtcgt
     1741 cactcaagga cgcctcttag caactatgac gccatgcgtt ttaaccattt taaaatcaaa
     1801 ggcctggcga ttttcgattc caatccttca ccgcaaaatc gccgcgttcc cgcgccggtc
     1861 gtttcatccc ctcgcctttt tacgtgtcct tccgtgactc gtgatcacgc ctttctttgc
     1921 aaacttcgcc cactcatgaa aaaaaggggg cgagtcccat tggacccgcc ccccggtgat
     1981 ttccgatctt cggcgatcgc ggttgactta cttccgcttc ttcgccgtct tgcccgcggc
     2041 cttcttcgcc ttcttcgcgc ccttggccga ttttttcgcc ggcttgcggc ttgccgcctc
     2101 cgccagcgcg gcctgcgccg ccgccagccg cgccaccggc acccggaacg ggctgcagct
     2161 cacgtaatgg aggccgattt cgtggcagaa catcaccgtc tgcgggtcgc cgccgtgctc
     2221 gccgcagatt cccagcttga tgtcggggcg cgtcttgcgg ctctcttcga tcgcgatctt
     2281 catcaggcgg ccgacgccgg tgcggtcgat cgtcgcgaac ggattgctcg ggatgatgtc
     2341 gagctcctgg tattgcggca ggaagttgcc cgagtcgtcg cggctcatcc ccagcgtcgt
     2401 ctgggtcagg tcgttcgtgc cgaagctgaa gaactcggcg tacttcgcga tctcgtcggc
     2461 caccagcgcg gcgcgcggga tctcgatcat cgtcccgacc aggtagttga tcttcttccc
     2521 cgtcgccttg cgcacttcct cggccacgcg gttgaccacg tcgagctgca gggccagttc
     2581 cttggggtag ccgaccagcg ggatcatgat ctccggcttg accttgatgc cctcggccat
     2641 gacttccgcg gcggcttcga agatcgcgcg aacctgcatc tccgtgatct cggggtagac
     2701 gttgccgagc cggcagccgc ggtggcccag catcgggttg gcctcgtgga gcgcattgac
     2761 gcgccgacgc acgtcgtcgg gcgacatctt caatttcgcc gccgtctcgt tgatcgccga
     2821 ttcctcgtgc ggcaggaact catgcagcgg cggatcgagc gtgcggatcg tcaccggcag
     2881 ccccttcatc gcccggaaga tgccggcgaa gtcctcgcgc tggtgcggca gcaggtcctc
     2941 cagcgccgcc cggcgatgct cctcgttctc ggccatgatc atccggcgga ccgagtagat
     3001 ccggtccccc tcgaagaaca tatgctcggt gcggcacagc ccgatgccga ccgcgccgaa
     3061 cgccaccgcg ttggccgcct ggccgggctg gtcggcgttg gtccgcacat ccagccggcg
     3121 gtacttgtcg gcccacttca tcagccggtc gaagttttga tacgtctggc tttgcttcgg
     3181 gtcgagcgtc ttgtcgatca gcacctggat gatctccgac ggcgccgtgt cgatttcccc
     3241 ttcgtagacc tcgcccagcg tgccgtcgat cgaaatccaa tcgccttcct tgatcgtccg
     3301 gccggccacg gtcatctgct tcctgccgta gtcgatgtgc aggtcgccgc agccgcagac
     3361 gcagattttg cccatctggc gggcgaccag cgcggcgtgc gagctgacgc cgccgcgcgc
     3421 cgtcaggatg ccttcggccg cgatcatgcc gcgcaggtcc tccgggctcg tctcgatccg
     3481 caccagcacg actttctcgc cgcggttggc ccaggcgact gcttcctcgg cgttgaacac
     3541 caccttgccc gtcgcggcgc cggggccggc cggcaggccc ttggccagca gccggttcgc
     3601 cttgaccgcc ttgtcgcgcg ccgcgcggtc gaacaccggc gccagcacct gcgtcaacga
     3661 gtcggccggg atccgctgga ccgccatctc ctcggtgatc agcccctcct tgaccatctc
     3721 acaggcgatc cgcaccgccg ccaggccggt ccgcttgccg ttgcgcgtct gcaacatgta
     3781 aagcgtgccg tcctcgatcg tgaattcgaa gtcctgcatg tccttgaagt gctgctccag
     3841 cgtccggcgc acggtctcca gctccttgtg cgccttgggc atctcctcct tgagcagcgc
     3901 aatcggcttc ggcgtgcgca cgcccgccac cacgtcctcg ccctgggcgt tgaccaggta
     3961 ctcgccgtag aacacttttt cgccggtggc cgggtcgcgc gtgaacgcca cgcccgtcgc
     4021 cgacgtgtcg cccatgttgc cgtagaccat cgtctgcacg ttgacggccg ttccccagtc
     4081 gtgcgggatg ccgtatttgc ggcggtagac aatcgcgcgg tcgttgttcc acgagccgaa
     4141 caccgcgccg atcgcccccc aaagctgctt ctccgggtcc tcggggaacg cctgccccgt
     4201 ccgctcgcga atcagcgcct tgaaccgccg gaccagttcc tgcaagtttt caaccgtcag
     4261 gtccagatcc gtctcggcgt tgttttcctt cttgatcgtc tcgatcgcgt gctcaaacgg
     4321 gtcgtgatgc tcgtccttgc gccgctggac ccccatcacc acgtcgccat acatctgcac
     4381 gaagcggcgg tagcagtccc aggcgaagcg cggattgccc gacttctcgg cgaccgcctc
     4441 ggtcgtcttc tcgttcagtc ccaggttcag gatcgtatcc atcatgccgg gcatcgaatc
     4501 gcgcgcgccg gagcggaccg aaacgagcag cggatccttc gcatcgccga acttcttgcc
     4561 catttccttt tcgacacggg ccagcgactt ggccacctga tccttcagct ccgccggata
     4621 cttccggcca ttctcgtaga aatacgtgca aacctcggtc gagatcgtga acccgggagg
     4681 caccggcaga ccgatgcggg tcatctcggc cagattggcc cccttgccgc ccagcagggc
     4741 gcgctgatcg ccgcgaccgt cggtcttcgt gcggccgaac gagaagacgt attgcttagc
     4801 catgaatcgg aatccccttg cgaaatgaaa tcagccagat ccgcgggtta tcgtgagcct
     4861 gtgattcgga gaaacggaac gcgggttcga caacaccggg ccctccaggg catgcaagcc
     4921 ccacatcttg aaaagcaccg cggcggttgt caacgattaa tcagatccgc gagtcgccag
     4981 accctccgca gttgcggtcg cgccccgcct gtggtccgat gaacgtggga atccgacgcc
     5041 cgggagtgcg ccccgatggt tgaagccggc cagatcctcg atcggatgga gaccgtgatc
     5101 gtcggcaagc ggccggtgct cgagttggtg ctggccgcaa tgctggcggg cgggcacgtg
     5161 ctgatcgagg atcgccccgg cgtggccaaa accctgctgg cgcgcacgct ggcccgtgtt
     5221 ttcacgctcg aattttcgcg cgtccagctg acgccggatc tgctgcccag cgacctgacc
     5281 ggcaccggca tctggaacga acacgagcgc gcgtttgttt tccagccggg gccgattttc
     5341 ggccagatcg tcctcgccga cgagctcaac cgcgccacgc cgcggacgca ggccggcctg
     5401 ctcgaggcga tggaggaacg cgccgtcacc gtcgacggcg tccgccaccc gctgccggcg
     5461 ccgtttttcg tcatcgccac gcagaatccg atcgagcagc agggcgtttt ccccctgccc
     5521 gaggcgcagc tcgaccgctt tctgatccag gtcgccatgg gatatcctgg ctatgaggac
     5581 gaggtgcgga tcgtgatgga ccaggccgcc gggcatccgc tcgatgagct gaagccggtg
     5641 gccggcgcgc gcgatttcgc cgagctcgcc gccgctgtcc agcaggtgca taccgacgat
     5701 tcggtgctgg ggtatctggt gcggctggcg cgcgccacgc gcgaacgcga cgacctgctg
     5761 ctgggcgcca gcccgcgggc gtcgctcgcg ctgcatcaca tggcgcgcac gctcgccgcg
     5821 atccgcggcg agaggttcct gcgccccgat cacgtcaagg cggtcgcgcc cgcggtgctg
     5881 cgccatcggc tgatcctgac gccccaggcg cggctggccg gcgtcgcccc cgacgacatc
     5941 atcgaggaac tcctggccga ggtcgaagcg ccgatttaca cgggctgagg agcaacgacc
     6001 gagatcggga aactccgtcc cgtgcgtcca ttgccccgtg cgtccgtccc cgtggagtta
     6061 acccctcatg ctccgagccg acccccgcat cggcggcgta ttctcctcgc gcaacatcct
     6121 gctggcgggc gtcggtttcc tcttcgcctg gctcctgctc ctgccggccg cgccggctca
     6181 ggcggcgctg gcggggttcc tgctcgcggc cggcggctgg ctctggctga ctcaccgcgg
     6241 tcagctccag gagcttcgca tcgagcggca tcaccgcccg cgcgtcttcg agaacgatcc
     6301 gctgaccgtc acactgcgcg tcgtgcgcgg ccggggctgg ccggtccaga tgctcgagat
     6361 cgaggatcaa ttcggcgcgt cgctccagat cgcccagcgc cacctggtcc ccggcctgca
     6421 cgagggatgg gaggtcctga ttcattatcc cgtcgtcgcc gagcgccacc gcggactcta
     6481 tctgatcggt ccggtcaacg tccgcgcggc cgatccgttc ggcatcttcc atgaacagcg
     6541 gcagatcgag acgctgacac ggctgacggt ctacccgcgg gcgcagccgc tcggggagta
     6601 cacgatcccc ggcccggaag cggcaccggg gccgtcgctc gatgtcgccg cgcggttggg
     6661 ccagggcgac gagatcctga ccgtgcgcga gtatcggccg ggcgatccgc cggcgcgaat
     6721 ccactggcgc acctcggccc gccgcggcgc cctgcacgtg atgcagctca accgcacggt
     6781 gcaggccgag ctggccgtct acatcgatct cagccgccgc gcgcgctatg gactgggggg
     6841 cgaggccacc accgaactgg ccatcaccgc cgccacgtcg atcctgaccc gcgcccatga
     6901 ggcgcgccac cgcgtctcgc tcgcctatgc tcagcgcgag cccgtcttct ttccgccggg
     6961 gtcggggctg gcgcacctgc acctgctgct cgatcgcctc gccatcgtcg agcccggcgg
     7021 cgagaccgat ttctggcggc acatcgtccc gcgggctctg gaactcccgg ccggttcgcg
     7081 cgcggtgctg atcgtcacgg ccggcgtgat gccgttcgaa gcggcgcgcg agatggtccg
     7141 gcgtctcgtc gcgcgccagg tcggcgtcga tctggtcctg atcgacgagg aaggctttat
     7201 ccggatctac aaggatcagg aggtggatct gcgccgcggc cagccgggtt tcgccgaatt
     7261 ggtcgaggcg ctctcgcagt ccggcgcgcg cgtctttccg ctgcggcgcg gcgcggcgga
     7321 actgcccccc cacccggtcg ccgccgcggc gcggcccgac tggtcccgcc gcggcgccgc
     7381 ctgacccctt cgaaacatcg tccctcgcct ctcgtcctcg gctctcaagt atcctcaaat
     7441 cctctcctcg aatgcccttt gattccaggt gtctcctccc tcatgtccct tccctgaggc
     7501 cgggggccgt ggtagtttgg cggaatgcgg accagttcgc ctcccgccat ccgtctgcgc
     7561 cgcgagccgt tcatcgagcg gtggggcggc tacctgatgg cgatctgcct gcacatcatg
     7621 ctcttcgcct tcctgatgat gcagccgatc gaaccgatcc ccgacgaggt ggagccaccc
     7681 gaccgcgtgc ggatcgcgct gcgaccgacg ccgacgcccg tcccgccccg ccgggtcgag
     7741 ccgccgaccg aacccgagcc gacgccggtc gtcgtgccct tccccgagcc caggcagatg
     7801 gccgcgcccg agccgccgga gccggtcctg cccgagccgc tcagcgagat cgaggaaacg
     7861 ctcgcggccg tcccgccgcc cgcgccacaa ccggcgcccc gggaggaaga agccccgcgg
     7921 ctgattgcgc tgctggagcc gacgcccgaa ccgcgcgtga tcgagccggt ggaaacgccg
     7981 acgctcacac ctacaccaac tccgacgccg acgccgacgc ccacgccaac cccgacgcca
     8041 acgccgacgc cgacgccaac accgacgccg acgccgacac cgacaccta
//
LOCUS       DAOPCO010000180         8052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8222_length_8052_cov_5.691009, whole genome shotgun sequence.
ACCESSION   DAOPCO010000180 DAOPCO010000000
VERSION     DAOPCO010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8222_length_8052_cov_5.691009"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLS90_11980"
     CDS             <1..426
                     /locus_tag="PLS90_11980"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPK03165.1"
                     /translation="WAVPFTLTLAEFRHEYHPGTQMARTYESDVIKREGGQEQFITIK
                     MNEPLRHAGYTFFQASFFEDPRSGVTSSTFAVVRNPADQWPLLACVIITAGLLLHFCQ
                     RLLRYLKGERARHGAGAPDPAPARPAGAGAARPQSEAAA"
     gene            423..2621
                     /gene="ccsA"
                     /locus_tag="PLS90_11985"
     CDS             423..2621
                     /gene="ccsA"
                     /locus_tag="PLS90_11985"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPK03166.1"
                     /translation="MKPLALDRLTRGLAAAVMLLPLIGGVLSAQPVAEGDDPHTGHTH
                     GAGESHAHHVHQHNAPVPPPPAPDWPAETLELFARTPIQAEGRVKPLSTFARFELVRL
                     SGKSRVRLEDGSRLDSLAWLLNCLFYPHVAQHYPVILVENPAVMRAVGLPDEEPRRRF
                     SLVELDPAAAAITELAQQAVQKEDKARTLFERQVLALYQNYAELNDLLQGLLFARTPI
                     VDEGLAAVAEQLEGISAREMRELPYSGLLNLLPRMIDRLPDPTDPAARERLNEPENQQ
                     LMGLIARIEAVANRSRALALIPPMSDREREEYYSAGTLLTAAAGGQAGPELALLGALE
                     QLPEQLGDPERFRATLAGFVSESRSAVAARGDAPHLELEIFFYRLKPFHWGLLLFLLG
                     FVLVGLVWLQPGNRWFAGAAWSATGAATLLLIVGITLRCIIRGRPPVSTLYETTLFIP
                     AVAIVVALAIEWINRRRVALSLAAALGVVGLFVANAYELREGGDTMGRLVAVLNSNFW
                     LSTHVTTVTMGYSAGLLAAALGHVYIFTRLVAPGRRELLRQVARMCYGVICFGLLFSF
                     VGTVLGGIWANYSWGRFWGWDPKENGALLIVLANLMILHARLGGYLRDLGICISAVLL
                     AIVVTLSWWGVNLLGIGLHSYGFTTGIMGRLLVFWAIESVVVAVGLLVHARDAAVRRA
                     TAGVAGAEGAAGAAGEYSGSGATGREGTEGTPATQGAGDRRIIEPINRFH"
     gene            complement(3014..6202)
                     /locus_tag="PLS90_11990"
     CDS             complement(3014..6202)
                     /locus_tag="PLS90_11990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK03167.1"
                     /translation="MILIDASLRNSTAVVVGMILTILFGYLAFIRIPIQLNPTIERPF
                     ITVETIYPGASATEVEQEITIPQEEQLASVENLLRLRSTSREGSAQIILEFEWGVNKD
                     LAALDVNKKLQLVEDLPEDAEEPQILTVNREEEETVNWLYVEVAPQVELTVNQLRQLV
                     DDLILPRLERVEDVGGVRRYGGAEREIHVLVDLDALAARDLSLNEIAAALRQENRNLR
                     GGDIDQGANRLIVRTLGQYSSLGQIGETVIKTGPAGPIRIRDVARVADNYKDQDVVVR
                     IDGSPSIAIGVVKKTGANTLEVAERVKAELKVLNESLAPRGLQIRVAYDASDYIWDSI
                     YQVRDNLIVGAILATVILWFFLRSVASTFIVGLTIPVCMVGTFVLLAAFGRSVNIVSL
                     AGLAFASGMIVDNGIVVIENIYRHRIELHKSILRASRDGAVEVWAPIVASTLTTLAVF
                     IPILFIEEEAGQLFRDIAFSISFAVGLSMFAAITMVPMLASRMLRLPVGERPELKADR
                     WWKIPAVAVHKVVDPILDRIARATSALFLGIVRLGLRSLAVSLAIIAILVVLFLFSLR
                     LTPEAEYLPTGNQNFVLGIFKFPPGLSLTGAEQLMLPIERQVLALPELERTFFVILRD
                     GPIFGAIIEKAQATKDNIQRIVGQLNGFAATQYPFPDVIPIIFQVPVFGGGQTGKSIT
                     VDIRGPQLRRLEELTNQITGQLRAIDGVEQVRPSLDLDNPELQVYPDRERLADLGLTS
                     SDLAETVEALVEGRITSLYREGGKEYDLKLKAADEEIRSPEDLRAATLALPGGAKVKL
                     ADVARVNRGLGPVTIEHLEQDRSVTITVNVAEDAPLEHVVNAVQQRIIDPLLPTLPYE
                     YNIELSGSADDLARTMQAMTGSFLLALLIIYLLMAALFRSFFYPLIIMFSIPLAMTGA
                     LLGIWLLDEQMNVITMLGYILLAGVVVNNAILLVDVTLSEVREGRGHAEAIEEAVRRR
                     MRPIFMTSVTSVLGMTPLALGQGAGAELYSGLGVAVVGGLILSTLFTLILIPLLMKIF
                     LGLRDGFARRVGREDWTEYASARALAELDEQI"
     gene            complement(6300..7778)
                     /locus_tag="PLS90_11995"
     CDS             complement(6300..7778)
                     /locus_tag="PLS90_11995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK03168.1"
                     /translation="MSTSSRRRILTASSVFLINLLFPALARPQMPVFSVQVDRAEQQD
                     VADWLEFTGTIEPWRVIDLAAQVEGRVQEVAIEEGERLRAGETVCTLDSEAIRIELDR
                     LAALQRKAEAELELRESGFRGEDVEAARRQLQAAQARLESARDEWERQAPLVAEGIIS
                     ESEGTRLRSAYREAQAEVQAAESELAKQEAGFRSEEIESARADVAIVRAQVAEAQRRL
                     RRSTVVTPVDGVVVERLREPGEWVAIGDAVARVAVLDPLKVRIELPQIYLSQVKEGLQ
                     ATIRVDGFPGREFQAEVARIIPLSGADTRNFPLWMRLANEDNLLRGGLFARVMLSLGE
                     QEPRITVPGEAIQIRGERLVVLVADPIPADMAAGGAPPGSPAHAPAEEGGPGPGGAAE
                     PGAGGPPGMPLMPTPNALIREVEVRTGPKVDGRVALEIVDGGPIRPGDQVVVLGGNQI
                     ANGMPVLVMQHNLLAPSTPGAEASGAAPSPTGDTAVAESPAG"
BASE COUNT         1255 a         2350 c         3067 g         1380 t
ORIGIN      
        1 tgggcggtgc cgttcacgct gacgctcgcg gagtttcgcc atgaatatca tccggggacg
       61 cagatggccc gcacctacga gagcgatgtg atcaagcgcg aagggggtca ggagcagttc
      121 atcacgatca agatgaacga gccgctgcgc catgccggct acacgttctt tcaggcctcg
      181 ttctttgagg atccgcgcag cggggtgacc tcctcgacgt ttgccgtggt gcgcaatccg
      241 gccgatcagt ggccgctact ggcatgcgtc atcatcaccg ccgggctgct gctgcacttc
      301 tgccagcggc tgctgcgtta tttgaagggg gagcgcgcgc gccacggcgc gggggcgccc
      361 gatccggcgc cggcgcggcc ggccggcgcg ggggccgcgc gtccgcaatc ggaggctgcc
      421 gcatgaagcc gctcgcgctg gacaggctga cccggggtct tgccgccgcg gtaatgctgc
      481 tccccttgat cgggggtgtg ctttcggcgc aaccggtcgc cgagggcgac gatccgcaca
      541 ccggacatac gcacggcgcg ggggaatcgc atgcgcatca cgtgcaccag cacaacgcgc
      601 cggtcccgcc tccgcccgcg ccggactggc cggcagaaac gctggagctg ttcgcgcgga
      661 cgccgattca ggcggagggc cgagtcaaac cgctctcgac gttcgcgcgt ttcgagctgg
      721 tccggctgag cggcaaatcg cgcgtcaggc tggaggatgg cagccggctg gattcgctcg
      781 cctggctgct caattgcctg ttttatccac atgtggcgca gcattatccg gtgatcctgg
      841 ttgaaaatcc ggcggtgatg cgcgccgtcg ggctcccgga cgaggagccg cggcggcgct
      901 tttcgctggt cgaactcgat ccggccgccg cggcgatcac ggagctggcg cagcaggccg
      961 ttcaaaaaga ggacaaggcg cgcacgctct tcgagcgcca ggttctggcg ctgtatcaga
     1021 actatgcgga gttgaacgat ctgttgcagg ggctgttgtt tgcgcgcacg ccgatcgtgg
     1081 acgaggggct ggcggcggtg gccgaacaac tggagggcat ctcggcgcgc gagatgcggg
     1141 agctgccgta ctccggtttg ttgaatctgc tgccgcggat gatcgaccgg ctgcccgacc
     1201 cgacggatcc ggcggcgcgg gaacggctca atgaacccga aaaccagcag ttgatgggtc
     1261 tgatcgcacg gatcgaggcg gtggccaatc gatcgcgcgc gctggcgctg atcccgccga
     1321 tgagcgaccg cgagcgcgag gagtattact ccgcggggac gctgctgacg gcggcggcgg
     1381 gaggccaggc cgggccggag ctggcgttgc tcggcgcgct ggagcaattg ccggaacagc
     1441 tgggcgatcc ggagcggttc cgcgcgacgc tggccgggtt tgtgagcgaa agccggtcgg
     1501 cggtcgccgc gcgcggcgac gcaccgcacc ttgagctgga gatttttttc taccggctca
     1561 agccgttcca ctggggtctg ctgctgtttc tgctggggtt cgttctggtg gggctcgtct
     1621 ggttgcagcc gggcaatcgg tggtttgccg gcgcggcgtg gagcgcgacg ggcgcggcga
     1681 cgctgctgct gatcgtgggg atcacgctgc gctgcatcat ccgcgggcga ccgccggttt
     1741 cgacattgta tgagacgacg ctgttcatcc cggcggtggc gatcgtcgtc gcgctcgcga
     1801 tcgaatggat caaccgccgg cgcgtggcgc tgagcctggc ggccgcgctc ggcgtggtgg
     1861 gtttgttcgt cgccaatgcg tacgagctgc gcgagggggg cgacacgatg ggccggctgg
     1921 tggcggtgct gaactccaac ttctggctgt cgacgcacgt cacgaccgtg acgatgggct
     1981 attcggccgg gctgctggcc gccgcgctgg ggcatgtcta tatcttcacg cgactggtgg
     2041 cgccgggccg ccgcgagctg ctgcggcagg tcgcccggat gtgttacggg gtcatctgtt
     2101 tcggcctgtt gttctcgttc gtcgggaccg tgctgggggg catctgggcc aattacagtt
     2161 gggggcggtt ctggggctgg gatccgaagg aaaacggcgc gttgttgatc gtcctcgcca
     2221 acctgatgat cctgcacgcg cggctggggg gatatttgcg ggacctgggg atctgcatca
     2281 gcgcggtgct gctggcgatc gtcgtgacgc tctcgtggtg gggggtcaat ctgctgggga
     2341 tcgggctgca cagctatggg ttcacgaccg ggatcatggg gcggctgctg gttttctggg
     2401 cgattgagag cgtcgtggtg gcggtggggc tgttggtgca tgcgcgggat gcggcggtcc
     2461 ggcgggcaac ggcgggggtc gcgggcgcag agggggcagc cggcgccgcg ggggaatatt
     2521 cagggagtgg ggccaccggg agggaaggga cggaagggac cccggcgacg cagggggcag
     2581 gggaccggcg cattatcgaa ccgataaatc ggttccattg aattggcgga tgcgaccgtg
     2641 ggttgaaatg gccgccgggg gcggtcgttt caagccacgg cttggttatc gaaccgctga
     2701 tgcggaacgc aggattccgg cctgcggccg tcaccctgcg ctttattccg gtcgtccgct
     2761 gcgcggactg gggcattcgc ccccttcggg gacggggggt ggggtgggcc ttccccaggt
     2821 tgagctgcgc tccaccagga ctaatcaaat tccgcctcta cgaggcgacc tctggcatcc
     2881 ctccgggata cgggggaggg attgccgggt gaccggtggt atcgctgcgc tcaaccaccg
     2941 gttactttct ggcaaccctg cgggatgccc agataaggga gtgtgaacgg ctcaatgggg
     3001 ggagcggggc gggtcagatc tgttcatcga gctcggcgag ggcgcgggcg gaggcgtatt
     3061 cggtccaatc ctcgcggccg acgcggcggg cgaagccgtc gcgcaggccg aggaagattt
     3121 tcatcagcag cgggatcagg atcagcgtga acagcgtgga gaggatgagg ccgccgacga
     3181 cggcgacgcc caagccgctg tagagctcgg cgccggcgcc ctggccgagg gcgagcggcg
     3241 tcatgccaag cacgctggtc accgaagtca tgaagatcgg gcgcatgcgg cggcggaccg
     3301 cttcctcaat cgcctcggcg tggccgcggc cctcgcgcac ctcgctcagt gtgacgtcca
     3361 ccagcaggat ggcgttattg accaccacgc cggccaggag gatgtagccg agcatggtga
     3421 tcacgttcat ctgctcgtcg agcagccaga tgccgaggag cgcgccggtc atggcgagcg
     3481 gaatcgaaaa catgatgatc agcggataga agaacgagcg gaagagggcg gccatgagga
     3541 ggtagataat gagcagggcg aggaggaagg agccggtcat cgcctgcatg gtgcgggcca
     3601 gatcgtcggc cgaacccgac agctcaatgt tgtattcata gggcagcgtg gggaggagcg
     3661 gatcgatgat ccgctgctgg acggcgttga cgacgtgctc gaggggcgca tcctcggcga
     3721 cgttgacggt gatcgtgacc gagcggtcct gttcgaggtg ttcgatcgtc acgggtccga
     3781 ggccgcggtt gacccgggcg acatcggcca gcttgacctt ggcgccgccg gggagggcaa
     3841 gggtcgccgc gcgcaggtcc tcgggcgagc gaatttcctc gtcggcggcc ttcagcttca
     3901 ggtcgtattc cttgcctccc tcgcggtaga gcgaggtgat gcggccctcg acgagggctt
     3961 cgaccgtttc ggccagatcg ctgctggtca ggccgagatc ggccaggcgc tcgcgatcgg
     4021 gatagacctg cagttcgggg ttgtccaggt cgagggaggg gcgcacctgt tcgacgccgt
     4081 cgatcgcgcg gagctgaccg gtgatctgat tggtgagttc ctcgaggcgg cgaagttgcg
     4141 ggccgcggat gtcgacggtg atcgacttgc cggtctgtcc gccgccgaag accgggacct
     4201 gaaagatgat ggggatgacg tcggggaacg gatactgggt ggcggcgaag ccgttgagct
     4261 ggccgacgat gcgctggatg ttgtccttgg tcgcctgggc cttctcgatg atcgcgccga
     4321 agatcgggcc atcgcgcagg atgacgaaga aagtccgctc gagctcgggc agggcgagga
     4381 cctggcgctc gatggggagc atcagttgtt cggcgccggt caagctgagg ccgggaggaa
     4441 acttgaagat gccgaggacg aaattctgat tgccggtggg caggtattcg gcttcggggg
     4501 tcaggcgcaa ggagaaaaga aagagcacga ccaggatggc gatgatcgcc aggctgacgg
     4561 cgagcgagcg caggccgagg cgcacgatgc cgaggaacag ggcggaggtg gcgcgggcga
     4621 tgcggtcgag aatggggtcg actaccttgt gcaccgccac ggcggggatc ttccaccagc
     4681 ggtcggcttt cagttcgggg cgttcgccca ccggcaggcg gagcatgcgg ctggcgagca
     4741 tcgggaccat ggtgatcgcg gcgaacatcg agaggccgac ggcgaaggaa atcgagaagg
     4801 cgatgtcgcg gaagagctgg ccggcctcct cctcgatgaa caggatcggg atgaagacgg
     4861 cgagggtggt gagggtggag gcgacgatcg gggcccagac ctcgacggcg ccatcgcgcg
     4921 acgcgcgcag gatcgatttg tggagctcga tccggtggcg gtagatgttc tcgatgacga
     4981 cgatgccgtt gtcgacgatc atgccgctgg cgaaggccag gccggccagc gagacgatgt
     5041 tgaccgagcg cccgaacgcg gccaggagga cgaaggttcc gaccatgcag accgggatcg
     5101 tgaggccgac gatgaaggtc gaggcgaccg agcgcaggaa aaaccagaga atcaccgtgg
     5161 ccaggatggc gccgacgatg aggttgtcgc gcacctggta gatggaatcc cagatgtagt
     5221 cggaggcgtc gtaggcgacg cggatctgga ggccgcgggg ggcgagcgat tcattaagaa
     5281 ccttgagctc ggccttgacg cgttcggcca cttccagcgt gttggcgccg gttttcttga
     5341 ccacgccgat ggcgatggac gggctgccgt cgatgcgcac gacgacgtcc tgatccttgt
     5401 aattgtcggc gacgcgggcg acgtcgcgga tgcggatcgg cccggccggc ccggtcttga
     5461 tcacggtttc gccgatctgg ccgaggctgg agtactggcc cagggtgcgg acgatgaggc
     5521 ggttggcgcc ctggtcgatg tcgccgccgc gcaggttgcg gttttcctgg cgcagggccg
     5581 ccgcgatttc gttgagcgac aggtcgcggg ccgcgagggc atccaggtcg acgaggacgt
     5641 ggatttcgcg ttcggcgccg ccgtagcggc ggacgccgcc gacgtcctcg acccgttcca
     5701 ggcgggggag gatcaggtcg tcgaccagtt ggcgaagctg attgacggtc agctcgacct
     5761 gcggggcgac ctcgacatag agccagttga cagtttcctc ctcctcgcga ttgacggtca
     5821 ggatctgggg ttcctcggca tcctcgggga gatcctcgac gagctggagt ttcttgttca
     5881 cgtcgagggc ggcgaggtcc ttgttcacgc cccattcgaa ctcgaggatg atttgggcgc
     5941 tgccctcgcg cgaggtggag cggaggcgca gcaggttttc gaccgatgcc agttgttcct
     6001 cctgggggat cgtgatctcc tgctcgacct cggtggcgga ggcgccgggg tagatcgttt
     6061 cgacggtgat gaagggccgc tcgatcgtcg ggttgagctg gatcgggatg cggatgaagg
     6121 ccaggtagcc gaagaggatg gtcaggatca tcccgacgac gacggcggtg ctgttacgga
     6181 gggaggcgtc gatcaggatc atgcggggtc gccgtcgtgg atcgtgggaa tcgggaaact
     6241 gggccggttc gccggcgggg gttgaaatcg ggacgcgcga caggggtcag gggtcgggct
     6301 catcccgcgg gggattccgc caccgccgtg tcgccggtgg ggctgggcgc ggcccccgat
     6361 gcctcggccc ccggcgtgct tggcgccagc aggttgtgct gcatgacgag gaccggcatg
     6421 ccgttggcga tctggttgcc gccgaggacg acgacctgat cgccggggcg gatcgggccg
     6481 ccgtcgacga tttcgagcgc gacgcgtccg tccaccttgg ggccggtgcg gacttcgacc
     6541 tcgcggatca gcgcgttggg ggtgggcatc agcggcatgc ccggggggcc gcccgcgccc
     6601 ggttcggcgg cgccgccggg gccgggcccg ccctcttcgg cgggggcgtg ggccggcgag
     6661 ccggggggcg cgccgcccgc ggccatgtcg gcggggatgg ggtcggcgac gagcacgacc
     6721 agccgctcgc cgcggatctg gatcgcctcg ccggggacgg tgatccgcgg ctcctgttcg
     6781 ccgaggctca gcatcacgcg ggcaaacagg ccgccgcgca gcaggttgtc ttcgttggcg
     6841 aggcgcatcc agagcgggaa attgcgggtg tcggcgcccg acagcgggat gatgcgcgcg
     6901 acttcagcct ggaattcccg gccgggaaac ccgtcgacgc gaatcgtcgc ctggagtcct
     6961 tctttcactt gactgaggta gatctggggc agctcgatgc ggaccttgag cggatcgagg
     7021 acggcgacgc gggcgacggc atcgccgatg gcgacccact cgccgggctc gcggaggcgt
     7081 tcgacgacga cgccatcgac cggggtgacg acggtcgagc ggcggagccg gcgctgggcc
     7141 tcggcgacct gggcgcggac gatcgccacg tcggcgcggg ccgattcgat ttcctcggag
     7201 cggaagccgg cctcctgctt cgccagttcg ctctcggcgg cctggacctc ggcctgggcc
     7261 tcgcgatagg ccgaacgcag gcgcgttccc tcgctttccg aaatgatccc ctcggcgacg
     7321 agcggggcct ggcgctccca ttcgtcgcgg gcgctctcca agcgggcctg ggcggcctgg
     7381 agctggcggc gggcggcttc gacatcctcg ccgcggaagc cgctttcgcg cagttcgagc
     7441 tcagcctcgg ccttgcgctg gagagccgcc agccgatcca gctcgatccg gatggcttcg
     7501 ctgtcgaggg tgcagacggt ctcgccggcg cggaggcgct cgccttcctc gatggcgact
     7561 tcctggacgc ggccctcgac ctgcgcggcc agatcaatca cgcgccaggg ctcgatcgtg
     7621 ccggtgaatt cgagccaatc ggccacgtcc tgctgctcgg cgcggtcgac ctgaacgctg
     7681 aagaccggca tctgcggccg ggcaagcgcc ggaaacagca ggttgatgag gaagacagag
     7741 gaggcagtga ggatacggcg gcgggaggaa gtcgacatcc gcgtgctccg cttcttcccc
     7801 tgcgaggatg cacctggttc tccccgcgac ggggacagac cgcgatatct taagccagcg
     7861 ggcaaacggc gtcaatcggg ctggtgacgg gggtgacggc gccgcaaggg ccctggggaa
     7921 atgagcggca tttctaacat gaatggcaca gtatgcgcta tggtcgcggg gggcaacggg
     7981 atgtgcggtt aaggcggaag caggcgattt tgcttgaaat ttccgctgca caggttacaa
     8041 ctgttgccaa ct
//
LOCUS       DAOPCO010000181         8017 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8260_length_8017_cov_5.949008, whole genome shotgun sequence.
ACCESSION   DAOPCO010000181 DAOPCO010000000
VERSION     DAOPCO010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8260_length_8017_cov_5.949008"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3637)
                     /locus_tag="PLS90_12000"
     CDS             complement(<1..3637)
                     /locus_tag="PLS90_12000"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPK03169.1"
                     /translation="MPRPPAQTPAPFRIALVWLTLILICLLVADPRGVRARNGESAPP
                     AAAGSIAIAPPPPPDQPVEEELEVEIPADATPQDLLERANALHAEGSFRLARAWFAAL
                     RDRADAPAALRDEAALMYYDSLWRERGIGVDVARDGLRRLMNDLPADSLLRARAAESL
                     ARLIQQFDAWRVDAEHLEPWLVAIKIYGESADVATARPRYLQLNLDLAEHLLRHHILP
                     VPKEPHLQALGILPPPPPENEPWRNDLVTWAARNVLKVADAPEQRAQALLLLGRLHLE
                     SWVPDEKARETWQAEAEGYFKQVIEIEPRTPWHDDALWLLAQWYNQRARYTDAAATAD
                     RFLELYERGESEYYDAAVGLRDEIRTPRIDLGAGRAFVPRSYIHLHIAYRNVSEAAVR
                     IERIAPEPYLQSRTSRLGMEDDPPPGEVVLEQALTGLVDKGDHAPGSHDKFLAPLAPG
                     IYRASIRSSQVDAELRPTYFLVSTVSVAIKQAQESLVAFVADAETGQPVAGADVWFRW
                     RIHTPNRNDTRYVQEKGVTDEQGLARAKRPAIGEQESYGSTLVLALKDGQPAAVESWS
                     GYFVPRVNQPWAQLYAYSDRPAYRPGEPIQWKAILRYRTEKEWLLPEQPTAWVVIRDA
                     RQQVAYEGEHRFSDYGTLAGTFAVDRTAALGMLQLQLFDKQGGSMLAGAALCRVEEYK
                     LPEFKVSVEPPAEQVQFGRELAFAIAAEYYFGGPVAGGAVEVVVHRRPFYPWWRPPMP
                     YPWLWEDGGYRAGGPVASSFMFWPRVEPEVVVLTETLELDEQGRARVVVPALSDEERM
                     DAEEREIWGYSYRVEARVVDASRREVTGEAAVKVARTAFAAYLNPQRYLYLPGDTAKI
                     EIKTLDPNDQPVSTEARVTIHKREWNATKVNDDKTTGGYDDTELFTRTIRTGADGMHL
                     FEFQPEQTGVYLVKLDGRDRFGAEVTAETNLFVADRDTTQTGYRSGGVEIITDKESYE
                     RGETAQVLLIVRRAGAVVWFAVESEGIHEQRVERVDGTSRLLAIPITQEHEPNIYLTA
                     LAMFDYSAFQTSKRIVVPPARRFLNVEIDSGKEELRPGETTTVRVKTTNHAGEPVSAE
                     ISLGVADQAVWAIQNDLVGDIRQAFWSETRGLAVQTQASASQYTIEYWQPRPGKPGEF
                     EAKRREDIPAEARDAAAGGIAADSDVYFARPRLSKGGERMALAEAMPAPTAQAAGLAF
                     DALGMEAG"
     gene            complement(3815..3993)
                     /gene="ssrS"
                     /locus_tag="PLS90_12005"
     ncRNA           complement(3815..3993)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLS90_12005"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4821..5963)
                     /locus_tag="PLS90_12010"
     CDS             complement(4821..5963)
                     /locus_tag="PLS90_12010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPK03170.1"
                     /translation="MKKTALSVMLWSALMPVLLLLAQPQWVSATIESTVNTAPPQTAP
                     AVVQNEGETLRRALLARLEFEELGLHLVGIAVFEGRPSCFIKHPGSSEQMAYTVGDVI
                     GGYTVEAIDDDSVTFARDGLRMWLMLGETSDGRHADQALAEAEALEAEAHDEELDGHS
                     AASLAADAEAFAEVQAAAAAPVEERIDIALDASRLGLEDGMTALRPAIIRKVENKTPP
                     DDLKNKTQRFKTAKLIEDGDVARPASSHSSAVASAMFQMPMKGRLTSSFGYRRHPIGG
                     QGRMHKGLDIAAPHGTTVRAAADGVVTKARYSSSYGYHVILRHGNGYETLYGHLSKRL
                     VKEGDRVKQGDPIGKEGDTGYSTGPHLHFEIRHNGKALNPLAYVKP"
     gene            6344..6646
                     /locus_tag="PLS90_12015"
     CDS             6344..6646
                     /locus_tag="PLS90_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03171.1"
                     /translation="MEYGDAAQLMRIIACATPIVALCGWIVLRGERRPDWPKTRRNLV
                     ILGLAGPVNLAGWWIFNGWLDRAGWRSVHAYIIAFIVFIVAGFATGFFSRLRRSER"
     gene            complement(6670..>8017)
                     /locus_tag="PLS90_12020"
     CDS             complement(6670..>8017)
                     /locus_tag="PLS90_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPK03172.1"
                     /translation="EAAPWRAWLDELAAAGRALHEGDRWYAAETERDPRALLRGRLEA
                     LGPVFDDDPLLAQLEADGHILRVRLAGRQAWCDRRLLARIQRYTLDRLRREIEPCTAA
                     QCLHFLAAWQHVTDEHRLEGPAGVAAVLDQLAGFEAPVAVWERAILPARVRDYQRDWL
                     DQLTLTGEFTWGRLWGSGNPALRATPICFLRREHLGRWLALAAPVDEAPLGAHARRVL
                     AALRGRGALFHAELMQAARLLPEHFEAGLAELIARGLLSCDSFGGLRLLTRPRHRRRV
                     RAAAPGRWALFRPSAAAPEADAALEGAAGPAADAADEAEFVARQLLRRYGLVFRRLLE
                     RERIPVPWRDLVRVLRRLELAGDVRGGRFVGGFSGEQYALPEAVALLRGLRRQARSSA
                     EPAPDGRNPSAPTDPTHFKGASSIPRVSAADPLNLAGILTPAERIAPTSRRLVDVV"
BASE COUNT         1262 a         2802 c         2679 g         1274 t
ORIGIN      
        1 cccctgcttc cattccgagc gcgtcgaagg ccaggcccgc cgcctgcgcc gtcggcgcgg
       61 gcatcgcctc ggcaagggcc atccgctcgc cgcccttgct caggcggggg cgagcgaagt
      121 agacatcgct gtcagccgcg atccccccgg ccgccgcgtc gcgcgcctcg gccgggatat
      181 cctcgcggcg ctttgcctcg aattccccgg gcttgcccgg cctgggctgc cagtattcga
      241 tcgtatactg gctcgcgctg gcctgcgtct gaaccgccag gccgcgcgtc tcgctccaga
      301 acgcctggcg gatgtcgccc accagatcat tctggatcgc ccagaccgcc tggtcggcga
      361 cgccgagcga gatctccgcc gagaccggct cgcctgcgtg attcgtcgtc ttgacgcgca
      421 cggtcgtcgt ttcgcccgga cgcagttcct ccttgccgct gtcgatctcg acgttcagga
      481 accggcgcgc ggggggcacg acaatgcgct tgctggtttg aaacgccgag taatcgaaca
      541 ttgccagcgc cgtcagataa atgttcggtt catgttcctg cgtgatcggg atcgcgagca
      601 gtcgcgaagt gccgtcgacc cgttcgacgc gctgctcgtg gatcccctcg gattccacgg
      661 caaaccagac gacggcgccc gcgcgccgga cgatcagcag gacctgcgcc gtttcgcccc
      721 gctcgtagct ctccttgtcg gtgatgatct ccacgccgcc cgagcggtag ccggtctggg
      781 tcgtgtcgcg gtcggcgacg aacaggtttg tctcggccgt cacctccgcg ccaaaccggt
      841 cgcggccgtc caacttcacc agatagacgc cggtctgttc gggctggaac tcgaacaggt
      901 gcatcccgtc cgcgccggtg cggatcgtcc ttgtgaaaag ttccgtgtcg tcatagccgc
      961 cggtcgtctt gtcgtcgttg accttcgtgg cgttccactc gcgtttatga atggtgacgc
     1021 gcgcctcggt cgagacgggc tggtcgttcg gatcgagcgt cttgatctcg atcttcgccg
     1081 tgtcgcccgg cagatagaga taccgctggg gattcaggta ggcggcgaac gccgtccgcg
     1141 cgaccttgac ggcggcctcg cctgtcacct cgcgccggct ggcgtccacc acgcgcgcct
     1201 cgacccggta gctgtagccc cagatctcgc gctcctcggc gtccatgcgc tcctcgtccg
     1261 acagcgccgg caccacaacc cgcgcccgcc cctgctcatc cagttcgagc gtctcggtca
     1321 ggacgacgac ctcaggttca acgcgcggcc agaacatgaa ggaggacgca acgggcccgc
     1381 cggcgcgata gccgccatcc tcccacaacc agggataggg catcggcggc cgccaccagg
     1441 gatagaacgg ccggcgatgg acgacgacct cgaccgcgcc ccccgccacc ggaccgccga
     1501 agtagtactc cgccgcgatc gcgaacgcca gctcgcggcc aaattgcacc tgctcggcgg
     1561 gcggttccac gcttactttg aactcgggca gcttgtattc ctcgacgcgg cagagcgccg
     1621 cgcccgccag catcgagcct ccctgcttgt cgaacaactg caactgcagc atccccagcg
     1681 ccgccgtgcg atcgaccgcg aacgtcccgg ccagcgtccc ataatccgaa aacctgtgtt
     1741 ccccctcgta ggccacctgc tggcgcgcgt cgcggattac cacccacgcc gtcggctgct
     1801 cgggcagcag ccattccttc tcggtccggt agcgcaggat cgccttccac tggatcggct
     1861 cgccggggcg ataggccggc cggtcgctgt aggcatagag ctgcgcccag ggctgattca
     1921 cacgcgggac aaaatagccg ctccacgatt cgaccgcggc cggctggccg tccttgagcg
     1981 ccagcaccag cgtggaacca taggattcct gctcgccgat cgccggccgc ttcgcccggg
     2041 ccagcccctg ttcatccgtc acgccctttt cctgaacgta gcgtgtatcg ttgcgattgg
     2101 gcgtgtggat ccgccagcgg aaccagacat cggcgccggc gacgggctga ccggtctcgg
     2161 catccgcgac gaacgcgacg aggctctcct gcgcctgttt gatcgcaacc gagacggtcg
     2221 agaccagaaa ataagtcggc cgcagctccg catcgacctg ggaggagcgg atcgaggcgc
     2281 ggtagatccc cggcgcgagc ggcgccagaa atttatcgtg gctccccggg gcatggtccc
     2341 ccttgtcgac caggccggtc agcgcctgtt ccaggacgac ctcgcccggc ggcggatcgt
     2401 cctccatccc gagccggctc gtccggctct gcagataggg ctccggcgcg atgcgttcga
     2461 tccgcaccgc ggcctcgctc acgttgcgat acgcgatgtg caggtgaatg taagagcgcg
     2521 ggacgaaggc gcggcccgcg cccaggtcaa tccgcggcgt gcggatctcg tcgcgcaggc
     2581 cgaccgccgc gtcgtagtat tcgctctcgc cccgttcgta gagttccaga aaccggtcgg
     2641 ccgtcgcggc ggcatcggtg taacgcgccc gctgattgta ccactgggcc agcagccaca
     2701 gggcgtcgtc atgccacggc gtgcgcggct cgatctcgat cacctgcttg aagtaaccct
     2761 cggcctcggc ctgccacgtc tcccgcgcct tctcatccgg aacccaggat tccaggtgca
     2821 gccggccgag cagcaggagc gcctgggcgc gctgctcggg cgcatcggcc accttgagca
     2881 cgttgcgcgc cgcccaggtg accaggtcgt tgcgccaggg ctcgttttcg ggcggcggcg
     2941 gcggcaggat ccccagcgcc tgcaagtggg gttccttcgg caccggcagg atgtggtggc
     3001 gcaggaggtg ttccgccaga tccaggttga gctggagata gcgcggccgg gcggtcgcca
     3061 cgtcggccga ttcgccataa atcttgatcg cgacgagcca cggctccagg tgttcggcgt
     3121 ccacgcgcca cgcatcgaac tgctggatga gccgggccag cgattcggcc gcgcgcgccc
     3181 gcaacagcga gtccgcgggc aggtcgttca tcagccggcg caggccgtcg cgggccacgt
     3241 caacgccgat gccccgttcg cgccacaggc tgtcgtagta catcagcgcg gcttcgtcgc
     3301 gcagcgccgc cggcgcgtcg gcccggtcac gcagcgccgc gaaccacgcg cgcgccaacc
     3361 ggaacgagcc ctccgcgtga agcgcattgg cccgctccag caggtcctgc ggcgtggcat
     3421 cggccgggat ctccacctcg agctcctcct cgacgggttg gtcgggcggc ggcggcggag
     3481 cgatggcgat cgagccggcc gccgcgggcg gagccgactc gccgttgcgc gcccgcacgc
     3541 cgcgcgggtc ggccacgagc aggcagatca ggatcagggt cagccagacc agtgcgatgc
     3601 ggaatggcgc gggcgtctgc gcgggggggc gcggcatctt cggcaagctc ccttcggcca
     3661 acggtggtgc tgtggtgaaa cggacgcgac gcgtcctgcc tgggatagac gcgctgcacg
     3721 cgaatttgtt tcaatcccag ggccgaagcg ccacaagatc cataccggaa tgcaaaaagg
     3781 cggaaccgcc cattcggctc cgcctttgat gatcatggtc cccgttcggc cgtattgccg
     3841 ggtcggtgag aacctgattc tcaccaggtg ggcgccgtga tccgccgctt ttcggatccc
     3901 cgcaaggggg cgtgcgagcc acgacggata ctcagctccc atttatcggt attggttcaa
     3961 acgccaccca aaataccaac ctcacaggga ccagattccg ccaacttgtc aaagagaact
     4021 cgcggcagcg ggagcccccg cgaggcagct gcccgccggt ccgcatcctg cctgccgtta
     4081 cgcctcagag catagccgat ctcgatcgcg gaatcaacct atctcggagc gaatcggggg
     4141 gaggggtggc tccggtgtca atctgaaaat gctcaaatac cggccgacat cccaggcaac
     4201 ggcgacataa cggccgtcgg gcgtcatggc caggcacacc ccgtcagccc gtcatgtttc
     4261 ggcagggcgc gggacccatt tgggaaattc ctccgcgtct cggcgtctcg gcgtgagatc
     4321 tgcgcgcgtc caggacggaa tgacggccga cgatccctgc gcggcgctga accggacggc
     4381 catgaatcaa gagagaaaca gtctcacgcc gagacgcgga gacaccgagg cgtgggaggt
     4441 gaagggatga ggcggagaca agatgggacg cccaccgaaa cctacggccg agaccatccc
     4501 ggattgatcc gccggccagt cccgtcaggg acggcagact tcagcccgct acttcagtgg
     4561 cgggaaaacg ggaacaacga atcgaacgag tcccgcaggg acggcagaac cgctcgactc
     4621 agtcacgatc acgagagtcg ggagccgagt tttggggcgg gccgatgggg gcgaggccac
     4681 tcaggagcgt ggtgctccca ggtttcggcc gggttttcga gagccgagga cgagggacga
     4741 acctggaatg agggcttgaa acggaaaggg cgcccccggt tcgggagcgc cctcgcttcc
     4801 tcagatggcc gtccaatgcg tcagggcttc acgtaggcca gcgggttgag cgccttgccg
     4861 ttgtggcgga tctcaaagtg caggtgcggg ccggtggagt agccggtgtc gccctccttc
     4921 ccgatcggat cgccctgctt gacgcggtcg ccttccttga ccagccgctt ggacagatgg
     4981 ccatagagcg tttcgtaccc gttgccgtgg cgcaggatga cgtgatagcc gtagctggag
     5041 ctgtagcggg ccttggtgac gacgccatcg gcggcggcgc gcaccgtcgt gccgtgcggc
     5101 gcggcgatat ccagcccctt gtgcatccgg ccctgaccgc cgatcgggtg gcgccggtag
     5161 ccgaagctgg aggtcaagcg ccctttcatc ggcatctgga acatcgccga cgccaccgcc
     5221 gaggaatggc tggaggcggg ccgcgccacg tcgccatcct cgatcagttt ggcggtcttg
     5281 aaccgttggg tcttgttttt caggtcatcc ggcggcgtct tgttctcgac cttgcggatg
     5341 atggccggcc gcagggcagt catgccgtcc tccaggccga gccggctggc gtccagcgcg
     5401 atatcgatgc gttcctcgac gggcgcggcg gcggcggcct gcacctcggc gaacgcctcg
     5461 gcatcggcgg ccaggctggc ggccgaatga ccgtccaact cctcgtcgtg cgcctcggct
     5521 tcgagggctt cggcctcggc cagcgcctgg tcggcgtggc ggccgtcgct ggtctcgccc
     5581 agcatcagcc acatgcgcag cccgtcgcgg gcgaaggtga ccgaatcgtc gtcgatcgcc
     5641 tcgaccgtat agccgccgat cacgtcgccg acggtgtagg ccatctgttc ggacgagccg
     5701 gggtgtttga tgaagcagga ggggcggcct tcaaagacgg cgatgccgac caggtgcagg
     5761 cccagttcct cgaactccag ccgggccagg agcgcccggc ggagggtttc cccctcattc
     5821 tggacgacgg cgggcgccgt ctgcggggga gcggtattga ctgtggattc gatggttgcg
     5881 ctgacccact gcggctgggc caaaagcaac agcacgggca tcagggctga ccacagcatg
     5941 acggagaggg cggtcttctt caagatacgc gatgacctcc ggtggcgttg agcgatgatg
     6001 ccgatccggc ctgcgcgccg tgacgggatc gttacatctt tctagcaagc gtccgacaat
     6061 tgggtttcaa agtgtgccgg gaagaatcag cggaaggcaa aaacctgtct tagtggcagg
     6121 aacgtccaat ccaccccggc gctttatctt gagacgtatc ggcgcggcgc cgatttctgt
     6181 tgagagcggg cgtcccttga ttcaatcgct gaacacccgc agtgcaaaac cggaaaaagc
     6241 cccagatcac gttccgggtt gatcggccag cccgaccaca ctaccctagg ggcccggggt
     6301 ttgacaagcc ctttcaaccc gccgacgcga ggcagcggac accatggagt acggcgacgc
     6361 cgcccagctg atgcgcatca tcgcctgcgc gaccccgatc gtcgcgctgt gcggctggat
     6421 cgtgctgcgc ggcgagcggc gccccgattg gcccaagacc cgccgaaatc tggtgattct
     6481 gggcctggcc gggccggtca atctcgccgg ctggtggatc ttcaacggct ggctcgaccg
     6541 cgccggctgg cggtcagtcc atgcttatat aatagcgttt attgtcttta ttgttgctgg
     6601 cttcgccacc ggcttttttt cccggctgcg ccgctccgag cgctaacatg ggggaatccc
     6661 ccgtatccgt cagacgacgt ccaccagccg ccggctcgtg ggtgcgatcc gctcggcggg
     6721 ggtcagaatc ccggccaggt tcagcggatc ggcggccgaa acccgcggga tcgaagatgc
     6781 ccccttgaag tgcgtcggat cggtcggagc cgacggattc cgtccatcgg gcgccggttc
     6841 ggccgagctc cgcgcctggc gccgcagccc gcgcagcagc gcgaccgcct cgggcagggc
     6901 atactgctcg ccggagaagc cgccgacgaa ccgcccgccg cgcacatcgc ccgccagctc
     6961 aagccggcgc agcacgcgca ccagatcccg ccaggggacg gggatccgct cgcgctcgag
     7021 cagtcggcga aacaccaggc cgtagcgccg gagcagttgg cgcgcgacaa actccgcctc
     7081 gtccgcggcg tcggccgccg gccccgccgc tccctccagc gccgcatcgg cctcgggggc
     7141 ggccgccgac ggacggaaca gcgcccagcg gccgggcgcc gcggccctga cgcgccgccg
     7201 atggcgcggc cgcgtcagca ggcgcaggcc gccgaacgag tcgcaggaaa gcagcccgcg
     7261 ggcgatcagt tcggccagtc ccgcctcgaa gtgctcgggc agcagccgcg ccgcctgcat
     7321 cagctcggcg tgaaacagcg cgccgcggcc gcgcaaggcg gccagcacgc gccgggcgtg
     7381 cgcccccagc ggcgcctcgt ccaccggcgc cgccagcgcc agccagcggc cgaggtgctc
     7441 gcgccgcagg aagcagatcg gcgtcgcgcg cagggccggg ttgccgctcc cccacagccg
     7501 cccccaggtg aattcgccgg tcagcgtcaa ctggtcgagc cagtcgcgct gataatcgcg
     7561 cacgcgggcg ggcaggatcg cgcgctccca gaccgccacc ggcgcctcga accccgcgag
     7621 ctgatcgagc acggcggcca caccggccgg cccctcgagc cggtgctcgt cggtcacgtg
     7681 ttgccacgcc gccaggaaat gcaggcattg cgcggcggtg cacggctcga tttcgcggcg
     7741 caaccggtcg agcgtgtagc gctggatccg agccagcagg cggcggtcgc accacgcctg
     7801 ccgcccggcc aggcgcacgc gcaggatgtg gccgtcggcc tccagttgcg ccagcagcgg
     7861 gtcgtcgtcg aagaccgggc cgagcgcttc gagccggccc cgcagcagcg cccggggatc
     7921 gcgctcggtc tcggcggcgt accagcggtc cccctcgtgg agcgcgcggc cggccgccgc
     7981 cagctcgtcg agccacgccc gccagggcgc cgcctcc
//
LOCUS       DAOPCO010000182         8004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8272_length_8004_cov_5.600075, whole genome shotgun sequence.
ACCESSION   DAOPCO010000182 DAOPCO010000000
VERSION     DAOPCO010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_8004_cov_5.600075"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PLS90_12025"
     CDS             <1..322
                     /locus_tag="PLS90_12025"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HPK03173.1"
                     /translation="RVLCEPRREAALVECLVRETSTFGVRRELTERWCLARRMETVAT
                     RLGPVAVKLGLWGERVIKASPEYEACRALAERHGVPLAEVYDLARAAAAERWGAAGGP
                     SHDA"
     gene            319..1356
                     /locus_tag="PLS90_12030"
     CDS             319..1356
                     /locus_tag="PLS90_12030"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPK03174.1"
                     /translation="MSPATRKRLYLLLGCVVSAAAIWWSTRGVSFSRVGRDFARYQYL
                     WLIPGLIAFYVSMYLRCIRWALLFRPHHALTGAGLFSPLMIGFAFNSILPARVGEFVR
                     VYLVGTRLGPGIPAAAATVVVERIFDGLMLLILLVLCLALLPPLDPGIAIPWGHYTLD
                     AGMINGGIRTLILGSSVLLAAVVLFLLPPFQRLMIGAVRRVPGLPERVRGAVERIILR
                     FAQGFHSMKNPWVLLQVVAHSFAIWVLVGVSNMAVAYGFGLEMNLLEATAIVALIAIA
                     ILIPAAPGYWGLYEAGGTFSLLVLGVTRSESTAFAYTLMTHLLQYVPIVVVGLLLALR
                     WHVRPVEAAQP"
     gene            complement(1430..2998)
                     /locus_tag="PLS90_12035"
     CDS             complement(1430..2998)
                     /locus_tag="PLS90_12035"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPK03175.1"
                     /translation="MTASAPVSFWEYRQRLKRRLTEGPGARAAEPESPYDGAHPAALN
                     PVSERLVQTIWAQGLFSPDGMRTVDGRALRVLEPGRWNGAAGPDFKGARLLLGPDTVD
                     GDVEIHLIPADWLAHGHQRDLDYNRVVLHVVLNNPDGGADDPLHNGKRVPRLELEPFI
                     FPDLETLRRSLSPDDYQYAQPEGLGRCHRLMAELPPAEIADFLDRAGDERLTAKMHRL
                     AEQAQSAELEQVFHQAVMMTLGAGPGRTLYYLLARRTPLKELMDFARELEPSHWNLGI
                     ETLLVHVAGLAPAAGDLANAPGEARDRAAALARLWGRFAPYWRDRLIPPTRRWFQGIR
                     PVNFPVRRLAGVALLLARAMRKGQMPLADLCDRIERAAPSLAEARPSRRRHPLIAELT
                     DWFRVSGEGHFWGTHYSFTAKPSPRTMDLIGESTARTLVFNALLPAALLRARHEKNDR
                     LEEAARRLYGLIPPLPPNHITQFMTRRLFGTAGPGAGLFTTERRQQALFQIFHHCCQA
                     EERHCDACYYFRPE"
     gene            complement(3010..4239)
                     /locus_tag="PLS90_12040"
     CDS             complement(3010..4239)
                     /locus_tag="PLS90_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPK03176.1"
                     /translation="MSFTMIHRFALSSLVVLTLAGAGAYFAPAQEPHKTPGAELKEIV
                     DPTQPVLGPEQTMLDAVAPFRRILWVTRWDYRTPEDVRQICYNAAAGRFTDILFQVRG
                     EGTVFYKSDLEPWAWELTGRTPAATGRDPGWDPLALAIQEGAKYGLRVHAYLNVLPGW
                     GQKQTAPPYTGQLYSVHRSWFMVDAAGNRMNPANWYAFLEPGLEEVRLHVTKVFADVA
                     RRYKVAGLHMDYIRYPMEKGDYGYTPRVVDDFRALVGGEPKRRPDEWDAFRRQHVTDL
                     VVGIRQAVQQINPKLELTAAVLADRTKALRAHQDALTWVREGYVDAILPMAYTDDLDL
                     FSDYLKAYQDEPIRKRTWPGILVEKGKAREQIEMTIQGDFPAVAVFAYGEVFSGHRQG
                     AQARAIYEKFMELAKRQ"
     gene            complement(5045..6877)
                     /gene="glmS"
                     /locus_tag="PLS90_12045"
     CDS             complement(5045..6877)
                     /gene="glmS"
                     /locus_tag="PLS90_12045"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPK03177.1"
                     /translation="MCGIIGMISRRDVGTALIEGLKNLEYRGYDSCGFATLCDGRFVI
                     HKEVGRVDRLDAYLPETEGCHIGMAHTRWATHGQPSEANAHPHLSYDGRFAVVHNGII
                     ENYSVLREYLRKQGIEFRSETDTEVIAHLIAHEYRNSTMQAVRDTLARLDGTFGLLIL
                     DRDDPHRMIAVRQGSPLVLGVGDGEFFAASDVSALIQHTRQVIYLDDGELAELTLTGY
                     RTATFTTDLTVVHETTEVDWEIEQIEKGGFEHFMLKEIFEEPQALRNTFAGRLRPDEG
                     IVKLGGIASLEDDFIREMRRILITSCGTAWHAGLIGEYILEELARIPVEVEYASEFRY
                     RNPLITQEDLLIVISQSGETADTLAALREGKTRGAEVMGICNVVGSTIAREAGRGIYT
                     HAGPEIGVASTKAFITQVAVMPMLALQWGRLRGSVSAQTVERMIAQLGRIPDHVAAVC
                     DQSDQIRTIAEQYCEHRNFLYLGRGINFPVALEGALKLKEISYIHAEGYPAAEMKHGP
                     IALVDENMPVVVIATKDPVYDKVISNIQEIRARRGRVIAVATEGDQEIAHHCNDVIYV
                     PEVDPLLTPIVNVVPLQLLAYHIAVLRGCDVDKPRNLAKSVTVE"
     gene            7186..7500
                     /locus_tag="PLS90_12050"
     CDS             7186..7500
                     /locus_tag="PLS90_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK03178.1"
                     /translation="MPVYEYEHLADPCAVGRFFERTQAMSEDALTTCPECGGPVKRLI
                     SLPNISTPTTDSELKSMGFTKLVRRDSGVYENVTALPGESKVMEAGKPETLPDLKRRG
                     LD"
BASE COUNT         1283 a         2523 c         2793 g         1405 t
ORIGIN      
        1 ccgcgtgctg tgcgaaccca gacgcgaggc ggcgctggtg gaatgcctcg tgcgcgagac
       61 ctccacgttc ggggtgcggc gcgaactgac cgagcgctgg tgcctggcgc ggcggatgga
      121 gaccgtggcg acgcggctgg ggccggtcgc ggtcaagctg ggactgtggg gcgagcgcgt
      181 gatcaaggcc agtccggagt atgaagcctg ccgcgcgctc gccgagcggc acggcgtccc
      241 gctggccgag gtctacgacc tggcgcgcgc cgcggcggcg gagcggtggg gcgcggccgg
      301 cggaccgagc cacgacgcat gagccccgcg acccggaagc gcctctatct gctcctcgga
      361 tgcgtggtgt cggcagcggc gatctggtgg tcgacgcgcg gggtgtcgtt cagccgcgtg
      421 gggcgggact tcgcccgcta tcaatatctt tggctcattc cgggcctgat cgccttttac
      481 gtttcgatgt atctgcgttg catccgctgg gcgctgctgt tccggccgca tcacgcgctg
      541 acgggggccg ggttgttcag cccgctgatg atcggcttcg cgttcaactc gatcctgccg
      601 gcgcgcgtgg gcgagtttgt ccgcgtctat ctggtcggca cgcggctcgg gccgggcatt
      661 ccagccgccg ccgccaccgt cgttgtcgag cggatcttcg acggcctgat gctgctgatt
      721 ctgctggtcc tctgtctggc gctgttgccg ccgctggacc ccggcatcgc catcccgtgg
      781 ggccactaca cgcttgatgc cgggatgatc aacggcggca tccgcacgct gatcctcggc
      841 agttcggtgc tgctggcggc ggtggtcctg ttcctgttgc cgccgttcca gcggctgatg
      901 atcggcgcgg tgaggcgcgt tccgggactg ccggaacggg tgcgcggggc ggtcgaacgg
      961 atcattctgc gcttcgcgca gggctttcac tcaatgaaga acccctgggt gctgctccag
     1021 gtcgtcgcgc attcgttcgc gatctgggtg ctggtgggcg tctccaacat ggcggtcgcc
     1081 tacgggttcg ggctggaaat gaatctgctg gaggcgacgg cgatcgtcgc gctgatcgcg
     1141 atcgcgatcc tgattcccgc ggcgccgggc tattggggcc tctacgaggc ggggggaacg
     1201 ttcagcctgc tggtgctggg cgtgacgcgc agcgaatcga cggcgttcgc ctatacgctg
     1261 atgacccatc tgctgcagta tgtgccgatc gtggtggtgg gcctgctgct ggcgctgcgc
     1321 tggcacgtgc ggccggtgga ggctgcgcaa ccgtaacttg cgtgcccggc cggcgaaggc
     1381 gccggtcgca catcggcgct cggctctcgt ggtgttggtg atgtggggat cactcggggc
     1441 ggaagtagta gcaggcgtcg cagtgacgct cttcggcctg gcagcagtgg tgaaaaatct
     1501 ggaacagagc ctgctgccgg cgctcggtgg tgaacaggcc cgcgcccggg ccggccgttc
     1561 cgaacagccg ccgcgtcatg aattgcgtga tgtggttggg cggcaggggc gggatcaggc
     1621 cgtagaggcg gcgcgcggct tcctccagtc ggtcgttttt ctcgtgccgt gcgcgcagca
     1681 gcgccgccgg cagcagggcg ttgaacacga gggtccgcgc cgtggattcg ccgatcaggt
     1741 ccatcgtgcg cggcgacggt ttggccgtga acgagtagtg ggttccccag aaatggcctt
     1801 cgccgctgac gcggaaccag tcggtcagct cggcgatgag cggatggcgt cggcggctgg
     1861 ggcgtgcctc ggccagcgag ggggcggccc gctcgatccg gtcgcacagg tcggccagcg
     1921 gcatctgccc cttgcgcatc gcgcgggcca gcagcagcgc cacgccggcc aggcggcgga
     1981 cggggaagtt gaccggccga atcccctgaa accagcggcg cgtgggcggg atcagccggt
     2041 cgcgccagta gggggcgaag cgtccccaca ggcgggcgag tgcggcggcg cggtcgcgcg
     2101 cttcgcccgg cgcgttggca aggtcgcccg ccgcgggcgc caaccccgcc acatggacca
     2161 ggagcgtttc gatccccaga ttccagtgcg agggctccag ctcgcgggca aagtccatca
     2221 gttccttgag cggcgtgcgc cgcgccagca ggtaatacag cgttcgcccc ggccccgcgc
     2281 cgagcgtcat catgacggcc tgatggaaca cctgttccag ctcggccgac tgggcctgct
     2341 cggccaggcg gtgcatcttg gccgtcaggc gttcatcgcc ggcgcgatca aggaaatcgg
     2401 cgatctcggc cggcggcagt tccgccatca gccggtggca gcgtcccagc ccctccggct
     2461 gggcatactg gtagtcgtcc ggcgagagcg accggcgcag cgtttccaga tctggaaata
     2521 tgaacggttc cagttcgagg cgcggcacgc gcttgccgtt gtgcagcgga tcgtcggccc
     2581 cgccgtcggg gttgttcagg acgacgtgca ggacgacgcg attgtaatcc agatcacgct
     2641 gatgcccgtg cgccagccag tcggccggga tcagatggat ttcgacgtcg ccatcgaccg
     2701 tgtcgggtcc gagcaacagg cgggcgccct tgaagtcggg cccggcggcg ccgttccagc
     2761 gccccggttc gagaacgcgc aatgcgcgcc cgtcgacggt ccgcatcccg tcggggctga
     2821 acagcccctg ggcccagatg gtctgaacca gccgttcgct gaccggattg agcgcggcgg
     2881 ggtgcgcgcc atcgtagggg ctttccggct cggcggcgcg cgcgccgggt ccctcggtca
     2941 accggcgttt gagccgctga cggtattccc agaagctgac gggcgccgac gccgtcatgg
     3001 gaagaagcct tactgccttt tggcgagctc catgaatttt tcgtaaatcg cccgggcctg
     3061 cgcgccctgg cggtggccgg agaagacctc gccgtaggcg aacaccgcga ccgcgggaaa
     3121 gtcgccctgg atcgtcattt cgatctgttc gcgggccttt cccttctcca ccaggatgcc
     3181 gggccaggtg cgtttccgga tcggctcgtc ctgataagcc ttcaaataat cgctgaataa
     3241 gtcaagatcg tccgtatagg ccatcggcaa aatggcgtcg acatacccct cgcggaccca
     3301 ggtcagggcg tcctggtggg cgcgcagtgc cttggtccga tcggcgagga ccgcggccgt
     3361 cagctccaac ttcggattga tctgctggac ggcctggcgg atgccgacga ccagatcggt
     3421 cacgtgctgg cggcggaagg catcccactc gtcggggcgg cgtttcggct cgccccccac
     3481 cagcgcgcga aagtcgtcga ccacgcgcgg cgtatagccg tagtccccct tttccatcgg
     3541 gtagcggatg tagtccatgt gcaggccggc gaccttgtag cggcgggcga catcggcgaa
     3601 gaccttcgtc acgtgcagcc ggacttcctc cagccccggt tccaggaagg cataccagtt
     3661 ggccggattc atccggttgc cggcggcgtc gaccatgaac cacgagcggt ggacgctgta
     3721 gagctggccg gtgtaggggg gcgccgtttg tttctgtccc catccgggca ggacgttcag
     3781 gtaggcgtgg acgcgcagcc cgtatttggc cccctcctgg atggcgagcg cgagcggatc
     3841 ccagccgggg tcgcggccgg tggcggccgg ggtgcggccg gtcagttccc aggcccacgg
     3901 ctccaggtcg ctcttgtaaa agacggtccc ctcgccccgg acctgaaaca gaatgtcggt
     3961 gaaacggccc gcggcggcat tgtagcagat ctggcggaca tcttcggggg tgcggtaatc
     4021 ccagcgcgtg acccagagga tgcggcggaa cggcgcgacg gcgtcgagca tcgtctgctc
     4081 ggggccgagc acgggctgcg tgggatcaac gatttccttc agttcggcgc cgggggtctt
     4141 gtggggttcc tgcgcggggg caaagtaggc gccggcgccg gccagcgtca gcacgaccag
     4201 cgatgagagc gcgaagcggt ggatcatggt gaacgacatg cagggcaaac cttgagccgg
     4261 cggtcgaggg ccgaccggga gtgggaatcg ggatagagga tcgtccgggg cgaggcatgg
     4321 cctgacttga acaacgatcc cggccgggcg gttcccctca aaccaccccg ccccgagaac
     4381 tggagcggcg aacgccgcga ttcattgtaa cagacgggcg gggggctgag aagatgggcg
     4441 cggccaaaat cgaccgattg cgaatattcg agcgccgagg acgaaagacg agagccggga
     4501 ccgcatttgg gggtgggtcg tttgggacga tgacgagtcg ccatgaaccg gcagtataca
     4561 accaacggat gaaagtgggg agtgaacggc cgattcgagc gccccagagc ttgccgggtc
     4621 cgtcagccgg gcggtcccgt cagggacgat agactacaac cccctacttc agtgcgggtc
     4681 gttccccaaa tcgcaatccg ctcaaggttc caaaaccaat gcaggcgatt ccgactcggt
     4741 tgccggctga gtccgcgaag cggaacgacg ggaataaagc gcagggtgga ggtcgaaggc
     4801 cgcaaccctg cgtcgccgcc tccaacgatt caagcccctg gaaggggcgg ccggaacatg
     4861 tctgaatttg ggcaatgaca aatacttcag gggcgggaac tcaggaatcg cgtcagcggt
     4921 tcgatatcca agccgtgggt tgaatgactg cccggcggcc attcaaccca cggtcaccgt
     4981 ccgtcagata gaagaaccga tttatcggtt cgataacaaa gattcgaggc gcgcgcggcg
     5041 ccggtcactc aacggtcacg ctcttggcca ggttgcgcgg cttgtccacg tcgcagccgc
     5101 gtaggacggc gatgtgatag gccagcaact gcagcggcac gacgttgacg atcggggtca
     5161 gcagcgggtc gacttcgggc acgtagatca catcgttgca gtggtgggcg atctcctgat
     5221 ccccctcggt ggcgaccgcg atcacgcggc cgcgccgcgc gcggatttcc tgaatattgg
     5281 aaatcacctt gtcataaacc ggatccttgg tagcgatgac gacgactggc atgttctcat
     5341 ccaccagcgc gatcggcccg tgcttcattt cggccgccgg gtagccctcg gcatggatgt
     5401 aggagatttc cttcagcttg agagcgccct ccagcgcgac cgggaagttg atcccgcgcc
     5461 ccaggtagag aaagttgcgg tgctcgcagt actgctcggc gatcgtccgg atctggtcgg
     5521 attgatcgca gacggccgcg acatgatcgg ggatgcggcc cagttgcgcg atcatgcgtt
     5581 cgaccgtctg ggccgagacg gagccgcgca ggcggcccca ttgcagggcg agcatcggca
     5641 tcaccgccac ctgggtgatg aacgccttgg tcgaggcgac gccgatttcc gggccggcgt
     5701 gcgtgtagat cccgcggccg gcctcgcggg cgatcgtcga gccgaccacg ttgcagatgc
     5761 ccatgacctc ggcgccccgg gttttgccct cgcgcagcgc ggcgagcgtg tcggccgtct
     5821 cgcccgactg gctgatgacg atcagcaggt cctcctgcgt gatcaggggg ttgcggtagc
     5881 ggaattcgga cgcatattcg acttcgacgg ggatccgggc cagttcctcg aggatgtatt
     5941 cgccgatcag gccggcgtgc cacgcggtgc cgcacgaggt gatcaggatg cgccgcatct
     6001 cgcggatgaa gtcgtcctcc aggctggcga tgccgcccag cttgacgatc ccctcatcgg
     6061 gccgcagccg gccggcgaag gtgttccgga gcgcctgcgg ctcctcgaag atctccttaa
     6121 gcatgaagtg ctcgaagccg cccttttcga tctgttcgat ctcccagtcg acctcggtcg
     6181 tctcgtgcac gacggtcagg tcggtggtga atgtggcggt gcgatagccg gtcagcgtca
     6241 gctcggccag ctcgccgtcg tcgaggtaga tcacctggcg ggtatgctgg atcagcgcgg
     6301 agacgtccga ggcggcgaag aactcgccgt cgccgacgcc gagcaccagc gggctcccct
     6361 ggcggacggc gatcatccgg tgcggatcgt cgcggtcgag gatcagcagg ccgaacgtcc
     6421 cgtcgagccg cgccagcgta tcgcgcaccg cctgcatcgt gctgttgcgg tactcgtggg
     6481 cgatcaggtg ggcgatcacc tcggtgtcgg tctcgctgcg aaattcgatg ccctgcttgc
     6541 gcaggtattc gcgcagcacg ctgtagttct cgatgatgcc gttgtgcacg acggcgaacc
     6601 ggccgtcgta gctcaggtgc gggtgggcgt tggcctcgga tggctggccg tgcgtcgccc
     6661 agcgggtgtg ggccatcccg atgtggcagc cctcggtctc cggcaggtag gcatccaggc
     6721 gatcgacgcg gccgacctcc ttgtggatca cgaaccggcc gtcgcacagc gtggcgaagc
     6781 cgcacgagtc gtagccgcgg tactccaggt tcttgagccc ctcgatcagc gcggtgccga
     6841 cgtcgcgccg cgagatcatc ccgatgattc cgcacatgaa gccgtacctg tccttccgtc
     6901 gattgccgga tcagaagggc cgattcccat gctcaatccg gatgctaaag agcatacccc
     6961 acacgccccc cctttttcaa agcagggcgt cgcgccagcc ggcgcgggct cgcccgccga
     7021 cgccgcgaaa ttccgagcgg ccgcttacct tggacgcatt ttccgctgag gggcgcgcgg
     7081 cttgcgttgg gcgggcgttg tgccgacaat aagggtggag gtgggtttcg ggccggcgcg
     7141 gaatcgccgg ccgccaccca gaccgcaacg ccggggatgt ttcccatgcc tgtctatgaa
     7201 tacgagcatc tggccgaccc ctgcgcggtg ggccgtttct tcgaacggac ccaggcgatg
     7261 agcgaagacg ccctcacgac ctgccccgag tgcggcgggc cggtcaaacg gctgatttcg
     7321 ctgcccaaca tttcgacgcc aacgaccgac agtgaactga agtcgatggg cttcaccaag
     7381 ctcgtccggc gcgactcggg cgtttacgag aacgtgaccg cgttgcccgg tgagagcaag
     7441 gtcatggaag cgggcaaacc cgagacgctg cctgatctga aacgccgggg attggattga
     7501 gcgcgggtcg gagcggctcg aatgggtcca gcccgcatcc gcaggggtgc cagagggtag
     7561 cccgtggttg agcgcggcga cgccaccggt tgacggccgc ccgacatccc cgcatcccgc
     7621 aaggatgcca ggctcctccc tgagccgcgc gaaatcccag aatcaacgca tgaacctgcg
     7681 cttcaacgag gattgaaaca cggggtgaac ggctgactcg aatgaccctg gccctgccgg
     7741 atccttccgc aggcagtccc gtcagggacg gttgacttca gcccgctact tcagtggcgg
     7801 gatccgggat ccccacaacg gctgagtccc gcagggacgg cagaaccgat caagcgatcc
     7861 cgagaatcgg gcaatatttt cgagtgagct tatcacgaca cctcggcgtc accctgaatg
     7921 gatgaaaacg ccccgcatgc gtcctttacc ttatgtccct tctgaaacga aaatcatcgt
     7981 cggaggtccc ggggacagtt tttt
//
LOCUS       DAOPCO010000183         7998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8281_length_7998_cov_4.866549, whole genome shotgun sequence.
ACCESSION   DAOPCO010000183 DAOPCO010000000
VERSION     DAOPCO010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8281_length_7998_cov_4.866549"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /locus_tag="PLS90_12055"
     CDS             complement(<1..1045)
                     /locus_tag="PLS90_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03179.1"
                     /translation="MSRTIENVRDGGFNVDAGWTENLAQYSGTGDTKVFDETTNPGAL
                     TLTYTNAAGSDETTVWVRGDVTLDEVGDFVQADMKTTGQRIGVCLAAATSFDQNRSNL
                     IYHGIRSDGIIRSHAYWGGGSELGDADVTVPGGVSDQWVTVQIRKTGANTFQLYWGLN
                     GVADTQVGGTFNYGNVSAPAGTETPKYPALMTDAGLGDFVGQFRNIIVGNINDPGVDN
                     FDDGALSLNWDWNNIPQAGSPVDTFSLQESGGRLLVDYTNLGGDQVTFIARDNVTLQD
                     GQSVVVAVRVTEGELSSAIGFGLGMATVGHGLWMDRSNTVFYVLRNDGLARANFFGNG
                     GVEIGDPLATLTGY"
     gene            complement(1829..3037)
                     /locus_tag="PLS90_12060"
     CDS             complement(1829..3037)
                     /locus_tag="PLS90_12060"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HPK03180.1"
                     /translation="MARRRLLSWILSVALLGVLPPAGQTAPEAPAAEMQWLQTADGRR
                     MGIALACDLRRTGEAWRLLGPEPGRPILQPLEALLPAAEGHASGFAVNGGYWNEHLFP
                     VGVCAGEAGIYAALSHPSGLALYAGGGVIGPLRTETILQPRPREAGEAVAWPAAADPT
                     AEATLPLIPEGTIPFNPAALPARTPYVIDFRTYTGPVQPRSRVRAVELEAATTAALRF
                     NQPVELATRASRIIEKDAIYSADERGLVLFVPLTDEADVDLPDRWRLEARLRAGDGED
                     AIAGRPVILATSAGPILMQGGRLNERLEADPARASRAERTLAGLDASGTRLWVVALMR
                     GPAGEAGVTLHEAAEIVQQLGASEILNLDGGGSTSVWAAGAQLALRALLPLQRPVHHI
                     LFFDGVVGGE"
     gene            3343..4326
                     /locus_tag="PLS90_12065"
     CDS             3343..4326
                     /locus_tag="PLS90_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK03181.1"
                     /translation="MGIIETEGLTKIYVRDVIDTEFGRLRIRLTNRKTVALQDLSLDV
                     QQGEIFGLLGPNGAGKTTAIKILMGIHFPTSGSARLMGKPLGDREVKKRIGFLPENPY
                     FYDYLKGWEFLDFYGQLYGMDKKTRRRKIERLLDQVGLTHAADRPLRGYSKGMNQRIG
                     LAQALMNDPEIVFLDEPQSGLDPLGRKEVRDIIVSLRDEGKTVFFSSHILSDAEMICD
                     RVAILYRGKLQSLGTLGSLLSQSVRDWEIVLRGVSDEFMDRWRPRLVRLEKSDGDFWV
                     IAKEEATTHELIREATAAGGVLIQLLPRRESLEDYFIRQVQGSPEPRGAAA"
     gene            4323..5216
                     /locus_tag="PLS90_12070"
     CDS             4323..5216
                     /locus_tag="PLS90_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03182.1"
                     /translation="MKEITAIALNTFKEAIRNRILYVILIFALLLILFSGVLSQLTIS
                     DRLQLIKSLGFAAINLFGVAIAVFVGVSLVYNELEKKTIYTIVSKPIGRWQFLLGKYF
                     GLLLTVWVNVLIMTLFFLISVHYNVGAMQGGGLASTLLASIGRGLLNLVAWNAFEPTQ
                     NLMFVIVVTCMELAIVTAFAILFSSFSTPTLSMFFTVLTFVAGRLNEHIIRFADTVLE
                     RARDEALAAAAPLTKPFAYWFAIGAAHVVPNLGAFHRSVTQAIYQNEVTLWPGTILYG
                     VLYTAGILCLAILIFSRRNFK"
     gene            5213..6466
                     /locus_tag="PLS90_12075"
     CDS             5213..6466
                     /locus_tag="PLS90_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03183.1"
                     /translation="MKRWGSFTALLAVTIALLGGAMRLQSEQIFRWRLIPFKFRDTLY
                     LPSSEYVRAVSVGYDVFMSDFLWLRMIQVFAASWTTPDSPETMKHYFDIITDLNPYNT
                     DVYKFAILAVGEEHKRHDMVKEIVNKGIQHNPLDYHIPYEGASYAFMSMEDLDQAKLY
                     VRMAKLDPNYPDFIDRWEGYFDIRQGRYEAAYSKFFREYIEAILADNPQLFDILRTQL
                     NRAMDEWFKSVIREAAVAWHDRTGLWSSVDELNAAGAFQGVRLPDVQFVRGALQTAIE
                     HDQGSGPLPPEQMDALIDRGVKTFDFLPLAPYDFIDPRYQGYVIWPYYYEDNPERFVL
                     AEIKAAQTMGLLASSVESRIQAYRDAHRGQCPPTLEALLGEEAALFTEHRDPFGGQWT
                     WDPATCQLGSTSFPSLIELGQLDVR"
     gene            6477..7568
                     /locus_tag="PLS90_12080"
     CDS             6477..7568
                     /locus_tag="PLS90_12080"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03184.1"
                     /translation="MPETTSRDHAGFVVAGLGKMGIMHAAMLGVIPGGRVAALVDRDE
                     RHAEHVRSMGIDAPVFGDLAACLDALRPEGVFITTPTFTHRSLFELCLTREIPAFCEK
                     PLAPTLADAQAMVQAARARPQLPVAVGFMLGHNPLFRKAADLLRRGVPGRIRSFRAVC
                     RLSQVFSPKKGWTFTREQAGGGVLINSGCHLLFVLDLLFGPPRSVFARASGVHNPVED
                     TLAALLEYPDGLWGTVEVTWSVPGHELQCHDIEVIGTQGTLEVGNDVLRLWLSQKHEE
                     YPAGWSLWPRAALEPRAPFNLSPDYCGDEFYLEVRDFVDAVRRQRPPRVGLDDALRVQ
                     TLIDAIYRSAEQRAPVSLAPPREAAHDAD"
     gene            7555..>7998
                     /locus_tag="PLS90_12085"
     CDS             7555..>7998
                     /locus_tag="PLS90_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03185.1"
                     /translation="MTLIDEVGGPDGDLLGLYRVLGAGGDGPANDPLRRELALRLADP
                     KTLHRCLARRFEQGFRAVVVWSDKVLFEALGRRHEPDLPRVIPIVPNLQGFMREAVEY
                     GMVGAGLRRAWRVGALSMAGMGLRGIGRLGEIKRRDFPTLLGSFIE"
BASE COUNT         1349 a         2971 c         2270 g         1408 t
ORIGIN      
        1 gatagccggt cagagtcgcc aacggatcgc cgatttcgac tcccccattg ccgaagaagt
       61 tcgcgcgcgc caacccgtcg ttgcgcagaa catagaaaac cgtgttggag cggtccatcc
      121 agagcccgtg gccgaccgtc gccatgccca gcccaaaacc gatggccgag gagagttcgc
      181 cctcggtcac ccgcacggcc accaccaccg attgcccgtc ctgcagcgtc acgttgtcgc
      241 gcgcgatgaa ggtcacctgg tcgccgccca gattggtgta atccaccagc agccggccgc
      301 cggactcctg caggctgaaa gtatcgaccg gcgaaccagc ctgcgggatg ttgttccagt
      361 cccagttgag gctcagcgcg ccgtcatcaa aattgtcaac gcccggatca ttgatgttgc
      421 cgacgatgat attgcggaac tgtccgacaa aatcgcccag acccgcatcg gtcatcagcg
      481 ccgggtattt cggggtctcc gtccccgccg gcgccgagac attcccgtag ttgaacgtcc
      541 cgccgacctg cgtgtcagcc accccgttca aaccccaata caactgaaac gtgttcgcac
      601 cggtcttgcg gatctggacc gtgacccact ggtcggacac accgccgggg accgtcacat
      661 ccgcatcgcc gagttcgctg ccgcctcccc agtacgcgtg ggatcgaata atgccgtcgc
      721 tacggatacc gtggtaaatc aggttggagc gattctgatc gaacgaagtg gcggcggcca
      781 ggcagacgcc gatgcgctga ccggtggtct tcatgtcggc ctgcacaaaa tcgcccacct
      841 catccagcgt gacatcgccg cggacccaga cggtggtctc gtcgcttccg gccgcattgg
      901 tgtaggtcag cgtcaaggca cctggattcg tggtttcatc gaataccttg gtgtcgccgg
      961 ttcctgaata ctgggccaga ttctccgtcc atcccgcgtc gacattgaat cctccatccc
     1021 gaacattttc gatggtgcgc gacatcccag gcgccgcgac cagacacgtg catattgcaa
     1081 acagtgcgat ccttctcgtc attttcctcc cgctcctttc caatgtatcc tctgcatttg
     1141 acccagatcg gtctctgcgt ttcccgagcc gcgccgattc cgctgttttg ccacggccac
     1201 aagtagccat gatgagttgt ggtatcgaac ctggcggcgc gcgcgccgcg gcatcgttca
     1261 gtcggccgcc ggcgtttggc atcgcccggc agacccgccg gcccgtcgcg cgggtcctgc
     1321 ttcgctcaaa agtgcgatgc ggcattgcac gtcgcctgcc cgataaagca atctaaagcg
     1381 ccacttcgag caacacggaa tcgagggcaa tatcccgaaa aatgagcctt ggcaagtaaa
     1441 gcctaggctc accttgctgc ccgtgtcaag aataaatatg accaatcaat gccttctcct
     1501 gaaaatgtcc gatatacctc accccttcac ctagtcgccc gacatgaaac cgctgagcgg
     1561 aatctgccgg gatcagacga gattgcaggg ctttacgcgg tattgatgag gagaatctag
     1621 acttttggcc gaagccgggc gcgccgcacc cccgctctgg gtagtgcctg caccctgccc
     1681 cagctggctg gttggagcga tgcccttggc ttcctgcacc catcgcaggt tggccagaaa
     1741 atcctccgac ggcaagtcga ggcgattggc gacggtggaa aaacccgctt ccgccggcgg
     1801 caacggtaag tgccggcatc cacttcggtt actccccgcc aaccaccccg tcaaaaaaga
     1861 ggatatgatg cacgggccgc tggagcggca gcagggcgcg cagtgcgagc tgcgcccccg
     1921 ccgcccagac cgatgtggaa ccgccgccat ccaggttgag aatctcgctc gcccccagtt
     1981 gctgcacgat ttcagccgct tcgtgcagcg tcacccccgc ttcccccgcc gggccgcgca
     2041 tcagcgccac cacccacagc cgcgtcccgc tcgcatccag ccccgccagc gtgcgttcag
     2101 cccggctcgc ccgcgccggg tcggcctcga gccgctcgtt gagccgcccc ccctgcatca
     2161 ggatcggccc ggccgacgtg gccaggatca ccggccgacc cgcgatggcg tcctccccgt
     2221 cgccggcgcg cagacgcgcc tccagccgcc accggtcggg caggtccacg tccgcctcgt
     2281 ccgtcagcgg cacgaacagc accagccccc gctcgtcggc ggaatagatc gcatcctttt
     2341 cgatgatccg gctcgcgcgc gtcgccagct cgaccggctg gttgaagcgc agcgccgccg
     2401 tcgtcgccgc ttcgagctcg accgccctga ctcgtgaacg gggctgcacc ggccccgtgt
     2461 aggtgcgaaa atcgatcaca tacggcgtcc gcgccggcag tgccgcgggg ttgaacggaa
     2521 tcgtgccctc tggaatgagc ggcaacgtcg cctccgccgt ggggtccgcc gccgccggcc
     2581 aggcgacggc ttcccccgcc tcccgcggcc gcggttgcag gatggtctcc gtccgcagcg
     2641 gcccgatgac cccgccgccg gcgtagagcg ccagtccgct cggatgcgac agcgccgcgt
     2701 agatcccggc ctcccccgcg cacaccccga ccggaaacag atgctcattc caatagccgc
     2761 cgttgaccgc gaagccgctc gcgtggccct ccgccgccgg cagcagcgcc tcgagcggtt
     2821 gcaggatcgg ccggccgggc tccggaccca gcaggcgcca cgcctcgccc gtgcggcgca
     2881 ggtcgcacgc cagcgcgatc cccatgcgcc gcccgtccgc ggtctggagc cattgcatct
     2941 cggccgccgg cgcctcgggc gcggtctgcc ccgccggcgg cagcaccccc agcagtgcca
     3001 cgctcaggat ccatgacaac aggcgacggc gggccactcc cattcctttc cacgcgctcg
     3061 gcaacggact gattcaggat tctgcggcaa acccgccacc gattcaaccc gcggcttctt
     3121 tcttgcggtc ggccgctgtt cgataccgat cacggttccg atgccaatcc cgatcctgat
     3181 ttctaattgc ccagctgttc ttcctgacct cgggtttcct tgcgcgtttg ccgcctcccg
     3241 ccgcattttc ggttgaacac atccgctgca tctcccatca tgattctgtc tgtgccagac
     3301 cgaatctccg ccgcccgggc ggcgggcgag aggccatgga gcatgggaat catcgaaacc
     3361 gaagggctga caaagatcta cgtccgcgac gtgatcgata cggagttcgg ccgactgcgc
     3421 attcgcctga ccaaccgcaa gacggtggcg ctgcaggatc tgtccctcga cgtccagcag
     3481 ggcgagatct tcggcttgct cggccccaac ggcgccggca agaccaccgc catcaagatc
     3541 ctgatgggca tccatttccc cacctcgggc tcggcccgcc tgatgggcaa gccgctgggc
     3601 gaccgcgaag tcaaaaagcg gatcggcttc ctccccgaaa acccctactt ctacgactat
     3661 ctgaagggct gggagttcct cgatttctac ggccagctct acggcatgga taagaaaacg
     3721 cgccgccgga agatcgaacg cctcctcgat caggtcggcc tgacccacgc cgccgaccgc
     3781 cccctgcgcg gctatagcaa gggcatgaac cagcgcatcg gcctggccca ggcgctgatg
     3841 aacgacccgg agatcgtctt cctcgatgag ccgcagtcgg gtctcgaccc gctcggccgc
     3901 aaggaagtgc gcgacatcat cgtctcgctg cgcgacgagg gcaaaaccgt cttcttctcc
     3961 tcgcacatcc tttccgatgc ggagatgatc tgcgaccgcg tcgccatcct ctaccgcggc
     4021 aaactccagt cgctcggcac gctcggcagc ctcctctccc agagcgtccg cgactgggag
     4081 atcgtcctgc gcggcgtcag cgacgaattc atggatcgct ggcgcccccg cctcgtccgg
     4141 ctcgaaaaga gcgacggcga cttctgggtc atcgccaagg aggaggcgac gacgcacgaa
     4201 ctgatccgcg aagcgaccgc cgccggcggc gtcctgatcc agttgctccc ccggcgcgaa
     4261 tcgctcgagg attacttcat ccggcaggtg cagggttcgc ctgaaccgcg gggagcagcg
     4321 gcatgaaaga aatcaccgcc atcgccctca acaccttcaa ggaagcgatc cgcaaccgga
     4381 tcctctacgt catcctgatc ttcgccctgc tgctgattct gttctcgggc gtgctcagcc
     4441 agctcaccat ctccgaccgg ctccagttga tcaaaagcct cggcttcgcc gcgatcaacc
     4501 tgttcggcgt cgccatcgcc gtcttcgtcg gcgtctcgct cgtctataac gaactggaaa
     4561 aaaagacgat ctacacgatc gtcagcaagc cgatcggccg ctggcagttc ctcctcggca
     4621 aatacttcgg cctgctgctg accgtctggg tcaatgtcct catcatgacc ctcttcttcc
     4681 tgatttcggt ccactataac gtcggggcga tgcagggggg cggcctggcc tcgaccctgc
     4741 tcgccagcat cggccgcggg ctcctcaacc tcgtcgcctg gaacgccttc gagccgaccc
     4801 agaacctcat gttcgtgatc gtggtcacgt gcatggaact ggccatcgtc accgccttcg
     4861 ccattctgtt ctcctcgttc tccacgccga cgctctcgat gttcttcacg gtgctgacgt
     4921 tcgtcgccgg ccggctcaac gaacacatca tccgcttcgc cgacacggtc ctcgagcgcg
     4981 cccgcgatga agcgctcgcc gccgccgccc cgctgaccaa gccgttcgcc tactggttcg
     5041 ccatcggcgc ggcgcatgtc gtccccaatc tgggcgcctt ccaccgctcc gtcacccagg
     5101 ccatctatca gaacgaagtc acgctctggc ccggcacgat cctctacggc gtcctctaca
     5161 ccgcggggat cctctgcctg gcgatcctca tcttcagccg gcggaacttc aaatgaagcg
     5221 ctggggttcg ttcactgccc tgctggccgt caccatcgcc ctgctggggg gagcgatgcg
     5281 cctgcagagc gagcagatct ttcgctggcg gctgatcccc ttcaagttcc gcgacaccct
     5341 gtatctcccc tccagcgaat acgtccgggc ggtctcggtc ggctacgatg tcttcatgtc
     5401 cgacttcctc tggctgcgca tgatccaggt cttcgccgcg agctggacca cccccgacag
     5461 ccccgaaacg atgaagcact acttcgacat catcaccgac ctcaacccct acaacaccga
     5521 cgtctacaaa ttcgccatcc tcgccgtcgg cgaggaacac aagcgccacg acatggtcaa
     5581 ggagatcgtc aacaagggca tccaacacaa tccgctcgat taccacatcc cctacgaggg
     5641 cgcctcctac gccttcatga gcatggagga tctggaccag gccaagctct atgtccgcat
     5701 ggccaagctc gatcccaact accccgactt catcgaccgc tgggagggct acttcgatat
     5761 ccgccaggga cgctacgaag cggcctacag caagtttttc cgcgaataca tcgaggcgat
     5821 cctcgccgac aacccgcagc tcttcgacat cctgcgcacg cagctcaacc gcgccatgga
     5881 tgaatggttc aagtcggtca tccgcgaggc cgccgtcgcc tggcacgacc ggaccggcct
     5941 gtggtccagc gtcgatgaac tcaatgccgc cggcgccttc cagggcgtcc gcctgcccga
     6001 cgtccagttc gtgcgcggcg ccctccagac cgccatcgag cacgaccagg gttcaggccc
     6061 gctccccccc gagcagatgg acgcgctcat cgaccgcggc gtcaagacgt tcgacttcct
     6121 tcccctcgcc ccctacgact tcatcgaccc gcgctaccag ggctatgtca tctggcctta
     6181 ttattatgag gacaatccgg aacgcttcgt cctcgcggag atcaaggccg cccagaccat
     6241 gggcctgctc gcctcgtcgg tcgagagccg aatccaggct taccgcgacg cgcacagggg
     6301 ccagtgcccc cccacgctcg aggcgctgct gggcgaggag gccgccctct ttaccgagca
     6361 tcgcgatccc ttcggcggcc agtggacctg ggatcccgca acctgccagc tgggatcgac
     6421 gtcgttccct tccctgatcg aactgggcca gctcgacgtc cgttaagccc cgccccatgc
     6481 cagaaaccac ttccagggac catgccggat tcgtcgtcgc cggactcggc aagatgggca
     6541 tcatgcacgc cgcgatgctc ggcgtgatcc ccggcggccg cgttgccgcg ctcgtcgatc
     6601 gcgacgagcg ccacgccgag cacgtccgct ccatgggaat cgatgccccg gtcttcggcg
     6661 acctggccgc ctgcctcgac gccctccgcc ccgagggggt cttcatcacc acgccgacgt
     6721 ttacccaccg ctcgctgttc gaactgtgcc tgacgcgcga gatccccgcc ttctgcgaaa
     6781 aaccgctcgc cccgaccctc gccgacgccc aggccatggt ccaggccgcc cgcgcccggc
     6841 cccagctccc tgtcgccgtc ggcttcatgc tcggccacaa cccgctgttc cgtaaagccg
     6901 ccgacctgct ccgccggggc gttccgggcc ggatcagatc cttccgcgcg gtctgccgac
     6961 tcagtcaggt cttcagcccc aagaagggct ggacgttcac ccgcgaacag gccggcggcg
     7021 gcgtcctgat caactccggc tgccatctcc tgttcgtgct cgacctgctc ttcggccccc
     7081 cgcgctcggt ctttgcccgc gccagcggcg tccacaatcc cgtcgaggat acgctcgccg
     7141 ccctgctcga atatcccgac ggcctctggg gtaccgtcga ggtcacctgg agcgtccccg
     7201 gccacgaact gcaatgccac gatatcgagg tgatcggcac gcagggcacg ctcgaggtgg
     7261 gcaacgacgt cctccggctc tggctcagtc agaagcacga ggagtatccc gcgggctgga
     7321 gcctctggcc gcgcgccgcc ctggagccgc gcgccccctt caacctcagc cccgattact
     7381 gcggcgacga attctatctg gaagtgcgcg atttcgtcga cgccgtccgc cggcagcgcc
     7441 ccccccgcgt cggcctggac gacgccctgc gcgtccagac gctcatcgac gcgatctatc
     7501 gctcggccga gcagcgcgcc cccgtctcgc tcgcgccgcc ccgggaggcc gcgcatgacg
     7561 ctgattgacg aagtcggcgg gcccgacggc gacctgctcg gcctctaccg cgtcctcggc
     7621 gccggcggcg acggccccgc caacgatccg ctccggcgcg aactggcgct tcgcctcgcc
     7681 gaccccaaaa ccctccatcg ctgtctcgcc cgccgcttcg agcagggttt ccgcgccgtc
     7741 gtcgtctgga gcgacaaggt cctgttcgaa gctctcggcc gccgccacga acccgacctc
     7801 ccgcgcgtca tcccgatcgt ccccaacctg cagggcttca tgcgcgaggc ggtcgaatac
     7861 ggcatggtcg gcgccgggct gcgccgcgcc tggcgcgtcg gcgcgctctc catggccggc
     7921 atgggcctgc gcggcatcgg ccggctgggc gagatcaagc ggcgcgactt ccccaccctc
     7981 ctcggttcgt tcatcgaa
//
LOCUS       DAOPCO010000184         7931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8348_length_7931_cov_5.381158, whole genome shotgun sequence.
ACCESSION   DAOPCO010000184 DAOPCO010000000
VERSION     DAOPCO010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8348_length_7931_cov_5.381158"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PLS90_12090"
     CDS             complement(<1..589)
                     /locus_tag="PLS90_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03186.1"
                     /translation="MSPFLSPAGSGQFTVLPVAARPSQPQPAARAGFRLALLLLAAAL
                     PASSARAQDNLILADDLAGSTSGVRSGGEFTDGGWKVTGTDDFIYWHIPTLTAGAVEF
                     DVTGLPPDDCRPENADKSELFHMYDYTFGNADTQYNGGYRDDPFKHFIRKTNCMDTRP
                     GKLNSLELVWQILPNYEEPDTAPLAWDATATYRFRE"
     gene            complement(854..4108)
                     /locus_tag="PLS90_12095"
     CDS             complement(854..4108)
                     /locus_tag="PLS90_12095"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPK03187.1"
                     /translation="MKLFRRHTRPDINPLDEVEYEKRRSVITNSDGSIVFEMRDAEVP
                     RAWSQLATDIIVSKYFRRAGLSGPEGRETSARQVVHRIVHTIRIAGEQMGRYFHSKED
                     ADTFEAELAHLLITQKGAFNSPVWFNCGLWHEYGIKGSGGNFAWDPATDAIEEIDNNY
                     SRPQCSACFIQSVEDDLMSIFDLIKTEAKIFKYGSGTGTNFSRLRSKHEKLSGGGTSS
                     GLMSFLEVFDRGAGATKSGGTTRRAAKMVCLDMDHPEIEEFITWKVREEEKARILIEH
                     GGLPADFNGEAYHTVSGQNSNNSVRITDEFMQAALEGKDWGTYGRVTGELIERKNAAD
                     LLAKICQAAWRCADPGVQFDTTINDWHTCPNTDKIRASNPCSEYMFLDDTACNLASIN
                     LLKYLDEEGEFDVEGFRHAVRLLILAQEILVDFSSYPTERIARNSHDYRPLGLGYANL
                     GAMLMVLGIPYDSPEGQAMCGAITAIMTGHAYRVSAEIAADVGAFPGYARNAKPFMRV
                     MHKHRDAVDRINPEFCPSYLLEAARQDWDEAIAHGEQHGYRNAQATVLAPTGTIGLLM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIVNQSVPAALRNLGYSSAEIQDILQYLLGTMT
                     LRNAPHINETTLRERGFTDDDLARVEKSLRGVFDLGSAFAPWVLGEDLLRRLGLNGQT
                     KEPGFSVLRFLGFTEAQIEEASDHICGRMTVEGAPHLREDHLVAVFDCANRCGRIGRR
                     FIAPMGHIRMMAAAQPFLSGAISKTVNLPNEATVDEIREIYVEGWRMGLKAIALYRDG
                     CKLSQPLSTSEKKPKAKEVEVAAKAPEAPAAAAFNRAVDEARAEAQPVQLKAPAPMRR
                     RLPQRRQGITVESRVGGHKVFLRTGEYEDGQLGEIFIDMHKEGAAFRSMMNCFAIAIS
                     KGLQYGVPLREFVDTFTFTRFEPQGPCDHPYVKLATSVVDYIFRVLALEYLGDDSMCQ
                     VKPEENIGSVVSVTESSAEAKALGWQPKTEDGGAEAAPGAAPAARPVRVVEPERVSAR
                     GGAQAQAKPAASGLESQMTTLMGDAPFCDSCGHITVRNGTCYKCLNCGNSMGCS"
     gene            complement(5236..6240)
                     /locus_tag="PLS90_12100"
     CDS             complement(5236..6240)
                     /locus_tag="PLS90_12100"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HPK03188.1"
                     /translation="MAETAPTQRPLIKRLMISIARWFDSTAGVNPAEKLAPRFELVRS
                     IPFIGLHLMCLGVLWVGVSPVAVGVAAFMYFIRMFAITGFYHRYFSHKTFKTNRFWQF
                     VFAVWGNTATQRGPLWWAAHHRHHHKFSDTEPDHHSPIIHTLYWSHMGWVSSASTYAT
                     DEQYVKDWLKFPELRLLNRFETVVPFLTGAAMFVLGWVLNTLWPHLGTSAMQMLIWGY
                     FISTVVLFHGTCTINSLNHLMGRRRYETGDNSRNSLLLAFVTLGEGWHNNHHYYPNTA
                     RQGFYWWEIDITYYGLKLLSWLGIIHDLKPVAREVRERNHIDKRAPAGLAPTAVGDQA
                     "
     gene            6698..7657
                     /locus_tag="PLS90_12105"
     CDS             6698..7657
                     /locus_tag="PLS90_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HPK03189.1"
                     /translation="MAATSPVPQAVGQPQAVGHEPAGRVLVSGSHGLIGAALLERLRA
                     DQARVVRLVHGATPRQGEQAISWEPEERRLDGAVLEGFDAVVHLAGESITGRWTVQKK
                     QRIRESRVAGTRLLCERLAAAAARPAVLICASAMGYYGDRGAEILTEENAPGGGFLAE
                     VCQAWEAATEPAREAGIRVALTRFGGVLSPRGGMLKMMLPFFKAGVGGPVGRGDQYVS
                     WIALEDVVRGILWLMRRADLAGPFNFAAPEAVTNREFARTLGRVLKRPAVTPAPAAAV
                     RLALGEMAEELLLASIRMTPARLQASGFEFRHPRLEDALRAML"
BASE COUNT         1261 a         2643 c         2644 g         1383 t
ORIGIN      
        1 cctcgcggaa gcgataggtc gccgtcgcat cccacgccag cggcgccgtg tccggttcct
       61 cgtagttggg caggatctgc cagaccagct ccagcgagtt gagcttcccg gggcgcgtgt
      121 ccatgcagtt ggtcttgcgg atgaagtgct tgaacgggtc gtcgcgatag ccgccgttat
      181 actgcgtgtc ggcgtttccg aacgtgtagt cgtacatgtg gaacagttcg gatttgtcgg
      241 cgttttccgg ccggcagtcg tcgggcggca ggccggtgac gtcgaactcg accgcccccg
      301 ccgtcagcgt tgggatgtgc cagtagatga agtcgtcggt gccggtgact ttccagccgc
      361 cgtcggtgaa ctcgcccccc gagcgaaccc cggaggtcga gccggccaga tcatcggcca
      421 ggatgagatt atcctgggcg cgggcgctgg aggccggcag ggccgcggcc agcagcagga
      481 gggcaaggcg gaacccggcg cgcgccgccg gctggggctg actcggtcgg gccgcgacgg
      541 gcagcacggt gaactggccg gaaccggccg gcgaaaggaa cggcgacatc gtcaactcct
      601 cccgaaaaac gggctgcgta tactcgagta tctattctgc cccccgcccc cctccccgcg
      661 caacccccgc ctccgcaaga cgccatctct tgctccaaaa ccatccctcg gcctcggctt
      721 tcgaaaccac tccgcaatta ccaaaagccg ggccgcctgt gcggcggtcc ggctttggac
      781 gttccaaggg ggtttcggcg tttgaaccag actttgtctc ccggccggac tccgagcgcc
      841 gccccgcgcg cctctacgag cagcccatcg aattgccgca attgaggcac ttgtagcatg
      901 tgccgttgcg cacggtgatg tggccgcaac tgtcgcagaa cggggcgtcg cccatcagcg
      961 tcgtcatctg gctttccagc cccgaagcgg ccggcttggc ctgcgcctgc gcgccgccgc
     1021 gcgcgctcac ccgctcgggt tccaccacgc ggaccggccg ggccgcgggc gccgcacccg
     1081 gcgccgcctc cgccccgccg tcctcggttt tcggttgcca gcccagcgcc ttggcctcgg
     1141 ccgacgattc ggtgaccgac acgacgctgc cgatgttctc ctccggcttg acctggcaca
     1201 tgctgtcgtc gcccagatac tccaacgcca gcacgcggaa gatgtaatcg acgaccgacg
     1261 tcgccagctt cacgtaggga tgatcgcacg gcccctgcgg ctcgaaccgc gtgaacgtga
     1321 acgtatcgac gaactcgcgc agcggcacgc cgtattgcaa gcccttgctg atcgcgatcg
     1381 cgaagcagtt catcatcgag cggaaggccg ccccttcctt gtgcatgtcg atgaagatct
     1441 cgcccagctg accgtcctcg tattcgcccg tgcgcaggaa gaccttgtgg ccgccgaccc
     1501 tggactccac ggtgatcccc tggcgccgtt gcggcagccg gcggcgcatc ggcgccggcg
     1561 ccttgagctg caccggttgg gcctcggcgc gggcctcgtc caccgcccgg ttgaacgccg
     1621 ccgccgccgg cgcctcgggc gccttggccg ccacctccac ctccttggcc ttcggcttct
     1681 tctcgctcgt gctgagcggc tggctcagct tgcagccatc gcggtagagc gcgatcgcct
     1741 tgagccccat ccgccagccc tcgacgtaga tctcgcggat ctcatcgact gtcgcttcgt
     1801 tgggcaggtt gaccgtcttg ctgattgcgc cggagaggaa cggctgcgcc gccgccatca
     1861 tccggatgtg ccccatcggc gcgatgaacc gccggccgat ccggccgcaa cggttggcgc
     1921 aatcgaacac cgccaccaga tgatcctcgc gcaggtgcgg cgccccctcc accgtcatcc
     1981 ggccgcagat gtggtcgctc gcctcttcga tctgcgcctc ggtgaagccc aggaagcgca
     2041 ggacgctgaa gcccggctcc ttggtctggc cgttcagccc gagccggcgc agcagatcct
     2101 cgcccagaac ccacggcgcg aacgcgctgc cgaggtcgaa gacgccgcgc agcgatttct
     2161 ccacccgcgc caggtcgtcg tcggtaaagc cgcgctcgcg cagcgtggtc tcgttgatgt
     2221 gcggcgcgtt gcgcagggtc atcgtcccca gcagatactg caggatgtcc tggatctccg
     2281 ccgagctgta acccaggttg cgcagcgccg ccggcaccga ctggttgacg attttgaagt
     2341 agcccccgcc ggccagtttc ttgaacttga ccagcgcgaa gtccggctcg atccccgtcg
     2401 tgtcgcagtc catcagcagg ccgatcgtgc cggtcggcgc cagcaccgtc gcctgcgcgt
     2461 tgcggtagcc gtgctgctcg ccgtgggcga tcgcttcatc ccaatcctgg cgcgccgcct
     2521 ccagcaggta gctcgggcag aactccgggt tgatccggtc gaccgcgtcg cgatgcttgt
     2581 gcatcacgcg catgaacggc ttggcgttgc gcgcgtaacc ggggaaggcg ccgacgtcgg
     2641 cggcgatctc ggccgacacg cggtaggcgt ggccggtcat gatcgccgtg atcgccccgc
     2701 acatcgcctg cccctcgggg ctgtcgtagg ggatccccag caccatcagc atcgcgccca
     2761 ggttggcgta gcccagcccc agcggccggt aatcgtggct gttgcgggcg atccgctcgg
     2821 tcgggtagct ggagaagtcg accagaatct cctgcgccag gatgagcagg cgcaccgcat
     2881 ggcggaaccc ctcgacgtcg aactccccct cctcgtccag gtatttcagc aggttgatcg
     2941 acgccagatt gcacgccgtg tcgtccagga acatgtattc ggagcacggg ttgctcgccc
     3001 ggatcttgtc ggtgttgggg caggtgtgcc agtcgttgat cgtcgtgtcg aactgcacgc
     3061 cggggtcggc gcagcgccag gccgcctggc agatcttcgc cagcaggtcg gcggcgttct
     3121 tgcgctcgat cagctcgccg gtgacgcggc cgtacgtgcc ccagtccttc ccctcgagcg
     3181 ccgcctgcat gaactcgtcg gtgatccgca ccgagttgtt cgagttctgg cccgaaacgg
     3241 tatggtaggc ctcgccgttg aagtcggccg gcagcccgcc gtgctcgatc aggatgcgcg
     3301 ccttctcctc ctcgcgcacc ttccaggtga tgaactcctc gatctcgggg tgatccatgt
     3361 cgaggcagac catcttggcc gcacgccggg tcgtgccgcc gctcttggtg gcgcccgccc
     3421 cgcggtcgaa cacctccagg aagctcatca gccccgagct ggtgccgccg ccggagagct
     3481 tctcgtgctt ggagcgcagg cgcgagaagt tcgtccccgt ccccgagccg tacttgaaga
     3541 tcttcgcctc ggtcttgatg aggtcgaaaa tcgacatcag gtcgtcctcg accgactgga
     3601 tgaagcaggc cgagcactgc ggccggctgt agttgttgtc gatctcctcg atcgcgtcgg
     3661 tcgccgggtc ccaggcgaag ttgcccccgc tccccttgat cccgtattcg tgccacaggc
     3721 cgcagttgaa ccagaccggc gagttgaacg cccccttctg cgtgatcagc aggtgggcca
     3781 gctcggcctc gaacgtgtcg gcgtcctcct tcgaatggaa gtaacggccc atctgttcgc
     3841 ccgcgatgcg gatcgtgtgg acgatgcggt ggaccacctg gcgggcgctc gtctcgcgcc
     3901 cctccgggcc gctcaggccg gcgcgccgga aatacttcga gacaatgatg tcggtggcaa
     3961 gctgactcca ggcgcgcggc acttcggcgt cgcgcatctc aaagacgatc gagccatcgg
     4021 agttggtgat caccgatcgg cgtttttcgt actccacctc gtccagcggg ttgatgtcgg
     4081 ggcgggtgtg gcggcggaac agtttcatgg ggaggccctt tcaggcgggc tcgtggtgtg
     4141 gtcggggccg accagccgtc gggcgtgggg gcaaaaccgg tctcagcgaa tgctcaatgg
     4201 cgacagctca gcggacacgc ccggcgaggg cgcgtcggcc ggagccttgc tccggcgcat
     4261 ttcgggtcat cttcgttcag gaggacaaag cacccatcgg gccggagcgc acggaccgac
     4321 catcagggct gccgaatttt cctcgcaagc gttccgagag cattctacag gagcgggtca
     4381 caacggtcca tcgggttcgc cttcacgggt tccacgggat cgccggcaac gctttaccag
     4441 agcggattca acgggtcgca accggagcag gggcgacggg tatcggatca ttcggcagcc
     4501 ctgagtcgcg gaggccccgg tcgctgttcc ggcgccgccg cgcggtcggg cgtcaggtgc
     4561 gcagtgcctg tcgcccggga tccccgttcc aggggaaccg gcccgatgcc ccccttctcc
     4621 ggcagggaag tcggcccggc cgtctctacc ccacccaagg ctgccagtga cagagggcgg
     4681 aggcgtcggg cccggcatcg gggttgaaat cgcgaggggg gacggttcgc ttcggcgggg
     4741 agacggaaag aaggccatgc agttcagttc gccgatagcg accggagccc ggtaagtcgc
     4801 gattgtgggg cggttataac gccggccccg ggagaagtca accccaaaaa aacaacatct
     4861 tgtcgtcctc ggtaaaggga ggtgcaatat attgtatccg ccggatccat ttgggaatcg
     4921 ggatcggcat cgccgccccg cgccgggatg cctcggccgc accgccgatc catgccgcct
     4981 ccatgggcca tcccggcccg aaatccagcg cgggagtggc gtcacgctca aaggcgcacc
     5041 cgagaattcg gctctcgtcg ttcaggtttc gaaaaccgcc tcatgaaaga agcaaccggc
     5101 cgatacctgg gagcgcaact ctcctgagta gagcttattt cttggggaaa ggaatattct
     5161 ggcgctgaaa tgaaaaatcc gtcggaccgg ttcgatccga cggatgattt gtcgcacggc
     5221 gggcttcgcg gcggattacg cctgatcgcc cacggcggtc ggcgcgaggc cggccggggc
     5281 gcgtttgtcg atgtgattgc gctcgcgcac ctcgcgcgcc accggcttca gatcgtggat
     5341 gatccccagc caggagagca gcttcagccc gtagtaggtg atgtcgatct cccaccagta
     5401 gaaaccctgg cgcgcggtgt tgggatagta gtggtgattg ttgtgccacc cctcgcccag
     5461 cgtgacgaac gccagcagca ggctgttgcg gctgttgtcg ccggtttcgt agcgccgccg
     5521 gcccatcagg tggttcagcg agttgatcgt gcacgtcccg tggaacagga cgacggtgga
     5581 gatgaagtag ccccagatca gcatctgcat ggcgctggtg cccaggtgcg gccagagcgt
     5641 gttgagcacc cagcccagga cgaacatcgc ggcgccggtc aggaacggca ccaccgtttc
     5701 gaaccggttg agcaggcgca gttcggggaa cttcagccag tccttgacgt attgctcgtc
     5761 ggtggcgtag gtcgacgccg acgagaccca ccccatgtgg ctccagtaga gcgtgtggat
     5821 gatcggcgag tggtgatccg gctcggtatc ggagaacttg tgatgatggc gatggtgcgc
     5881 cgcccaccac agcggcccgc gctgcgtggc cgtgttcccc cagacggcga acacgaattg
     5941 ccagaaccgg ttcgttttga aggtcttgtg cgagaagtag cggtggtaga atccggtgat
     6001 ggcgaacatg cggatgaaat acatgaacgc ggcgacgccc accgccaccg ggctgacccc
     6061 gacccacaac acgcccaggc acatcaggtg cagcccgatg aacgggatgc tgcggacgag
     6121 ttcgaaacgc ggggccagtt tctcggccgg gttgacgccg gccgtcgagt cgaaccagcg
     6181 ggcgatgctg atcatcagcc gcttgatcag cgggcgctgg gtcggtgcag tctcagccat
     6241 caggcttgct ctccattcat ggtcggggta cggcgtcggt tcagcggagg cggaaggcgc
     6301 ccggccggac aacgccgcgg ttcaagctat tgaatcggtt ggacgcgcgg tcgatctgag
     6361 tgatttgcgg tggcgggtcg atcaacgacg gatgtgcaat caaatccagc gagcgaatga
     6421 tgaaagcctg ctttcggtga gacatcgttc gcatcccaat cactcgcccg ctcccgccgg
     6481 ggggcgcggg cgccgtgggg tggaacggag gcgctgcggg cgcccgttca tccggaacag
     6541 accctagcac gtccggtaag gattggccaa gaataattga ttgcgatgtg aggtaatcgg
     6601 gacgccggcc tccccaaccc gtccgcgacc ccgcccgcga cggatggcat aaattcagct
     6661 tcctgaacct tgcgtgattt cgccaaagga tcttcccatg gccgccactt cccccgttcc
     6721 ccaggccgtc ggccagcccc aggccgtcgg tcacgaaccc gccgggcgcg tcctcgtctc
     6781 cggatcgcac ggcctgatcg gcgccgccct gctcgaacgg ctgcgggccg accaggcgcg
     6841 cgtcgtccgg ctggtccacg gcgccacccc gcgccagggc gagcaggcga tctcctggga
     6901 gcccgaggaa cgccgactcg acggcgccgt tctggagggc ttcgatgccg tcgtccacct
     6961 ggccggcgag agcatcacgg gccgctggac cgtccagaag aaacagcgga tccgcgaaag
     7021 ccgcgtcgcc gggacgcggc tgctctgcga gcggctggcc gccgccgccg cccggccggc
     7081 ggttctgatc tgcgcctccg ccatgggcta ctacggcgac cgcggcgccg agatcctgac
     7141 tgaggagaac gctcccggcg gcgggttcct ggctgaggtc tgccaggcgt gggaagccgc
     7201 aaccgaaccg gcccgcgagg ccggaatccg cgtggcgctg acccgcttcg gcggcgtgct
     7261 cagcccgcgc ggcggcatgc tcaagatgat gctgccgttc ttcaaagccg gcgtcggcgg
     7321 ccccgtcggc aggggcgatc aatacgtgag ctggatcgcg ctggaggacg tcgtgcgggg
     7381 gattctgtgg ctgatgcggc gcgcggacct tgccggcccg ttcaatttcg ccgcgcccga
     7441 ggccgtcacc aaccgcgagt tcgcccggac gctggggcgc gtgctcaagc ggccggccgt
     7501 gacgcccgcc cccgccgcgg ccgtccggct ggccctgggc gaaatggccg aggagctcct
     7561 gctggcgagc atccgcatga cgcccgcgcg gctgcaagcc tccggcttcg agtttcgcca
     7621 cccccggctg gaggacgccc tccgggcgat gctgtgaagg cggatccgtc gcgcgtcctc
     7681 gggcgggatg gaaatgccgg tggggagtcg atttcgattt cgatcccgat cctgatttca
     7741 atccagatcc cggtctgttt cgtatgcggc ggcgccgcgc cgtgaatgga gttcgagagc
     7801 cgaggacgag ggacgagagc cgaattttgg ggaaggcctt cgggagcgat tacgagaacg
     7861 aggacgaggg acgagagacg cgttttcagg acgagggacg aacctctcag aggggttcga
     7921 agagttcgcc t
//
LOCUS       DAOPCO010000185         7892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8393_length_7892_cov_4.105142, whole genome shotgun sequence.
ACCESSION   DAOPCO010000185 DAOPCO010000000
VERSION     DAOPCO010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8393_length_7892_cov_4.105142"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLS90_12110"
     CDS             complement(<1..180)
                     /locus_tag="PLS90_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03190.1"
                     /translation="MSLATPAVELFGGPLGRGRAALERGGVAGGLRLGGACGAGRAPA
                     GGGVATVRPSLAAIWP"
     gene            179..2374
                     /locus_tag="PLS90_12115"
     CDS             179..2374
                     /locus_tag="PLS90_12115"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPK03191.1"
                     /translation="MQHLVGRTIDGYRIEKMLGAGGMGAVFLAHQISLDRDVAMKVLP
                     TRFAQNPELLARFTREALSAAQLNHHNIIQVYDVGSNEDVHYITMEFVRGQDLSQLVK
                     QDGRLRLDDAAGYILQAARGLKYAHERGIIHRDIKPNNLMLNEHGVVKIADMGLAKMR
                     GKHDTQGQLLRPTREELMNTPADDNLTLADVAMGTPAYMAPEQARDAASVDARADIYS
                     LGCTLYYLAAGRNPFDGGSAFELITKHMKEPLTPLDAHIKGVSPAINTIIGRMLAKNP
                     DERYPTMEGVIRDLEKYLGLDSEKGPYTPREQHLGILDAALKAYYTAPALRLRKAVRL
                     AFLVAVPVLFLLALFSGRYVLAGGFLGFGVLTAAARFIIHGVMTKDYLFRRVRGLFFG
                     MPLVSWLKVGAGALLALAVLYVLGWLWSWLGFLLFAVAAAGLYQVLVEQKLRQQRAGA
                     IEQTQEMLRELRVRGVSEETLHDFVARFSGKDWEEFFEQLFGYEALVVARGRWAAADQ
                     VESRNQHAAWREPVNRWVTAQEEKRRAARERKQLAKVEAERLKARGVSETDARRQAGE
                     AATRILDEQKQQHDRRERDQKALAERGAALDQAIKSSRRKKLGAAGWFRVARAVAGLL
                     MLVAPFAEGLGLPDLLVGPIRGIYAPLGAGVSIWGAAAGAALLVSALSVRMVAPIIIV
                     LGAFFVACANLAAEQSAALVPGAAVTIGLALLAAGLALHVMNKLAGKAF"
     gene            2887..4092
                     /locus_tag="PLS90_12120"
     CDS             2887..4092
                     /locus_tag="PLS90_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03192.1"
                     /translation="MSALRVLILEDDQDIIDLIQSVLEPDFECYSAPNGDIGLDAAIH
                     GEPDLIICDIMMPVMDGREFLRRLRLVPGLESIPVIILSALTSRDHIRDGYALGAALY
                     VPKPIEPKRFRRNLEMFVVDHELKVRPKRMSLAELRHKTFKASATPPPPRRQQAAFLM
                     PDLTHTSTPIEFTSEGLLRNPHPPRDPERGASQPTAKTFSSLRPSAPARPPLRAAASG
                     SPAGGDPSARSDKGGARSVVGQKIRMMIIEDDMDTCQLLKAEFERDHDVLIANDGVAG
                     VEQAVKYKPDIFVIDGILPRLNGFQVTTMLRKHRDFFRSPIILISGKATPRDRQHAER
                     LGVTHFIAKPFTGAQMRRLVEATEQRPDFSVHKDRLDMKVIRLEKLQHLETHRTQRNF
                     RADAVENSY"
     gene            4919..5461
                     /locus_tag="PLS90_12125"
     CDS             4919..5461
                     /locus_tag="PLS90_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK03193.1"
                     /translation="MSPEPTEPIQYAHLPDNIIVIRVLGKGTHLQSPSLRYVFDQTRD
                     AAPPPRYIIDLDRCTTMDSTFMGTLASIGLHQVERTGSRVIVTNIQEHVLRLLNTLGL
                     KYILDLRANHDQTVNRLGLGEEAFTPAAGPRLDKLDRIVLMIEAHERLIDVDSQNEVK
                     FQGVLRTLRQSLDEEQQRPS"
     gene            5523..6800
                     /locus_tag="PLS90_12130"
     CDS             5523..6800
                     /locus_tag="PLS90_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPK03194.1"
                     /translation="MIRRRTLPNGVQVVTEQLDTVRSCAIGVFLQVGSRHERPAENGI
                     SHFLEHMLFKGTSRRTARQIADATNYLGGNLNAFTTQELICLHAKTVDSKAPSTLDLF
                     GEMLIDSIFPPEEIRRERQVVLEEFKMYEDTPDELSVDLFLRNLWPDHPLGRPVIGSR
                     TSISRLSRAGIQRFLRRVFHPSRLLIVLSGSFDSRACSRAINQWFGKVEPAGAAAGRL
                     GGRPPRAPRSRRTHLRRRIEQTHFCLGVPAPDRTSPDRFAFSLLNMILGGGMSSRLFQ
                     EIREKRGLAYSIGSFTQSFTDAGCFAVSGGTSPDTYGEVLRITLEELQRITEEPAPEE
                     ELVMAREQLVDSLLMSLENTESRMLRLADSLLNFGRVVPVDEILTQLRRVEPDDVRRI
                     AAKYLTGASLALASIGPNAKMEPISENLRQVAA"
     gene            6816..7652
                     /locus_tag="PLS90_12135"
     CDS             6816..7652
                     /locus_tag="PLS90_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03195.1"
                     /translation="MNSAIHIFHRFTGVGIAGLILAAHLAFVAGCQRVRTDAALPNLV
                     QVSAEGYVDPATVAPRAAAVAGDDLTSAAVVVHDGSITDPGQRVLAARAAARRQALRG
                     IGEQLQHLRAADGRPLLLTLRAADSPAIAQLNELLDRHAETTYTEFNGRSKALVLLPA
                     ARLNEFLAAAGGAPESSSLPLTAEEDQVLRAQAYEAALRDARKQLRDVIPIVPIEPNG
                     PTIGEVIERRPSLVNELNALMLATQPDTVEYPEPGRCRMTLSFDMGQARELARAAFRQ
                     KD"
     gene            7659..>7892
                     /locus_tag="PLS90_12140"
     CDS             7659..>7892
                     /locus_tag="PLS90_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPK03196.1"
                     /translation="MPQPEMIPSSDSPRIVLASASPRRQALLKAGGVRFEVRVSALDE
                     SPYHHPSAPRASALKTAMAKASAVAPNLPRDTIV"
BASE COUNT         1308 a         2982 c         2335 g         1267 t
ORIGIN      
        1 cggccagatg gccgccagcg acggccgcac cgtggcgacc ccgcccccgg ccggcgcgcg
       61 gcccgctccg caagccccgc ccagccgcaa cccgccggcc acgccccccc gctcgagcgc
      121 cgcgcgcccc cgccccagcg gccccccgaa gagctccacg gccggcgtcg caagggacat
      181 gcagcatctg gtcggacgca ccatcgacgg ctaccgcatc gaaaaaatgc tcggggccgg
      241 cggcatgggc gccgtcttcc tggcgcatca gatctcgctc gaccgcgacg tcgcgatgaa
      301 ggtcctcccg acccggttcg cccagaatcc ggaactgctc gcccgcttca cgcgcgaggc
      361 gctcagcgcc gcccagctca atcaccacaa catcattcag gtctacgacg tcggcagcaa
      421 cgaggacgtc cactacatca cgatggaatt cgtccgcggc caggacctct cgcagcttgt
      481 caagcaggac ggccggctgc ggctcgacga cgccgccggc tacatccttc aggcggcgcg
      541 cgggctcaaa tacgcccacg agcgcggcat cattcaccgc gacatcaagc cgaacaacct
      601 gatgctcaat gagcacggcg tggtcaagat cgccgacatg ggcctggcaa agatgcgcgg
      661 caagcacgac acccaggggc aattgctgcg ccccacccgc gaagaactga tgaacacgcc
      721 ggccgacgac aacctcaccc tcgccgatgt ggcgatggga accccggcct acatggcgcc
      781 ggagcaggcc cgcgacgccg cgtcggtcga tgcccgcgcc gacatctact cgctgggatg
      841 cacgctctac tacctcgcgg ccgggcgcaa tccgttcgac ggcggctcgg cgttcgagct
      901 gatcaccaag cacatgaagg agccgctgac gcccctcgac gcccacatca agggcgtctc
      961 gccggcgatc aacacgatca tcgggcggat gctcgccaaa aaccccgacg agcgttaccc
     1021 cacaatggag ggggtcatcc gggacctgga gaaatacctc ggcctcgact ccgagaaggg
     1081 cccgtatacc ccccgcgagc agcatctggg cattctcgat gccgcgctga aggcctatta
     1141 caccgcgccg gccctccgcc tgcgcaaggc cgtgcggctg gcgtttctcg tcgccgtgcc
     1201 ggtgctgttc ctcctggcgc tgttcagcgg cagatacgtc ctggccggcg gattcctggg
     1261 gttcggcgtg ttgactgcgg ccgcgcgctt catcatccac ggcgtgatga cgaaggacta
     1321 cctcttccgc cgcgtgcgcg ggctgttctt cggcatgccg ctcgtcagtt ggctcaaggt
     1381 gggcgcgggg gccctcctcg cgctggccgt gctgtatgtg ctgggctggc tctggtcgtg
     1441 gctcgggttt ctgctgttcg cggtcgcggc cgccgggttg tatcaggtcc tcgtcgagca
     1501 gaagctgcgc cagcagcgcg ccggcgcgat cgaacagacg caggagatgc tgcgcgagct
     1561 gcgcgtgcgc ggtgtctcgg aggaaacgct ccacgacttc gtcgcccgtt tctccggcaa
     1621 ggattgggag gaattcttcg agcagttgtt cggctacgaa gccctcgtcg tggcgcgcgg
     1681 ccgctgggcc gcggccgatc aggtcgaatc gcgcaaccag catgccgcct ggcgcgagcc
     1741 ggtcaaccgc tgggtcacgg cccaggagga gaagcgccgc gctgcccgcg aacgcaagca
     1801 actggccaag gtcgaagccg agcgcctcaa ggcccgcggc gtcagcgaaa ccgatgcgcg
     1861 gcgccaggcg ggcgaagccg cgacgcggat cctcgacgag cagaaacagc agcacgaccg
     1921 ccgcgagcgc gaccagaagg ccctggccga gcggggggct gccctggacc aggcgatcaa
     1981 gtcctcgcgc cgaaaaaagt tgggcgccgc cggctggttc cgcgtcgcgc gcgccgtcgc
     2041 cggcctgctg atgctcgtgg ccccctttgc cgaggggctc ggcctgcccg acctgctggt
     2101 tgggccgatc cgcggcatct atgcgccgtt gggggctgga gtgtccatct ggggcgcggc
     2161 ggcgggcgcc gcgctcctgg tgtcggcgct ttccgtccgc atggtcgcgc cgatcatcat
     2221 cgtgctgggc gcgttttttg tcgcctgcgc gaacctcgcc gccgaacaga gcgcggcgct
     2281 ggtccccggc gcggccgtca cgatcggcct ggccctcctc gccgccggct tggccctcca
     2341 cgtgatgaac aagctggcgg gcaaggcgtt ttgagcggac gcatgacggg gtagccggtg
     2401 accggaggca tcccccccac cggcgtggga ccggccctcg gtgtgcgacc ggcgccccgt
     2461 gtgcgaccgg cgctcccgcc ggtcatcccc gccgcatccc ggaggggtgc cggatactag
     2521 ccggtggttg agcgtagcgc taccaccggt caccgagccc cccgttcccc cgcctcccgg
     2581 agggatgcca gctgccacgc gtgccaactc gcatcccccc cgtcgccgcc ctcaatgccc
     2641 catcgccacc ccccctgtaa ccatctcgca tcacgtagct cgcaacccgc gacggatcat
     2701 ccttccggcc cgcgatccga ccgacgcgcc cccaccactc acccgaattc acttgcttcg
     2761 cgcgccgggg cggagggaac atgggaattg ttgtaagaga ggatccctgg cgagctgaaa
     2821 tgaccaccgc cccctacgga catttcggcg ctcgcgcccc cttcgaacgc ataaggaggc
     2881 aggaccatgt ccgccctgcg cgtgttgatc ctcgaagacg atcaggacat cattgacctg
     2941 atccaatccg tcctcgaacc cgacttcgaa tgctattcgg cccccaacgg cgatatcggc
     3001 ctcgacgccg ccatccacgg tgagcccgat ctgatcatct gcgacatcat gatgcccgtc
     3061 atggacgggc gcgagttcct ccgccgcctg cgtctcgtcc ccggcctcga gtcgattccc
     3121 gtcatcattc tctcggccct cacctcgcgc gatcacatcc gcgacggcta tgccctcggc
     3181 gcggcgcttt acgtccccaa gccgatcgag ccgaagcgct tccgccgcaa cctcgagatg
     3241 ttcgtcgtcg accatgagct caaggtccgc cccaagcgca tgagcctcgc cgagctgcgc
     3301 cacaagacgt tcaaggcgtc cgcgacgccc ccaccgccgc gccgccagca ggccgccttc
     3361 ctgatgcccg acctgacgca tacctcgacc cccatcgaat tcaccagcga gggactgctg
     3421 cgcaatccgc atccaccccg tgaccccgag cgcggcgctt cccagcccac ggccaagacc
     3481 ttctcctcgc tgcgcccctc ggcccccgcc cgcccgccgc tccgcgcggc ggcttccggt
     3541 tcgcccgccg gcggcgatcc ctccgccagg tccgacaagg gcggcgcccg ctccgtcgtc
     3601 ggccagaaga tccgcatgat gatcatcgaa gacgacatgg atacctgtca gttgctcaag
     3661 gccgagttcg agcgcgacca cgacgtgctg atcgccaacg acggcgtcgc cggcgtcgaa
     3721 caggccgtta aatacaaacc cgacatcttc gtcatcgacg gcatcctccc ccggctcaac
     3781 gggtttcagg tgacgacgat gctgcgcaag catcgcgatt tcttccgctc gccgatcatt
     3841 ctgatctcgg gcaaggccac cccgcgcgac cgccagcacg ccgaacgatt gggcgtgacg
     3901 cacttcatcg ccaagccctt caccggcgcc cagatgcgcc gcctggtcga ggcaaccgaa
     3961 cagcgtcccg atttttccgt ccacaaggac cggcttgaca tgaaggtcat ccgcctcgaa
     4021 aaactgcagc acctggaaac ccaccgcacc cagcgcaact tccgggcgga tgccgtggag
     4081 aacagctact gaccacccct ttcgcgttcg ccctgtccgg catcaaacgc cccccggcgc
     4141 gtgcaccccg ggcgaatcat ctctccgact gatccgcctg attgactgga taggcccgcg
     4201 aagctcacac cggaataacg cgcttgtcat tccagaaatc ccaatccgct caaggcttcc
     4261 aactgcaatg ctggcggtcc gatcagctga cccggtcagt ccgcgaagcg gacgacggga
     4321 ataaagcgca aggtgacggc cgcaggccgg aaccctgcgt cgccgcgccc aacgattcaa
     4381 gcccctggaa ggggcggccg gagttggtct gatttgggga atgacaggta cttcacgcaa
     4441 aaaaccagga agtcccctga ggacggccgg accgctccaa tcgatcgtct gaatgcggac
     4501 agtaatttcg aacccgcgcc agcgggctct cttcccggcc ccctgggggt ccttgacggt
     4561 ggcgccgggc ctcgggcccg gtgacccaac gccctaagta ttcctcaacc gttttcaacg
     4621 ggttccctgc aacaccgcca ctccaatgtc agcctggccc ccgccggcat ccctggccct
     4681 cacccgcggc gcgcgacctc tatggtttat cctgtcttct tccctcggcg gcttcgcggc
     4741 tccgcgtgag accggaaaac cctcacgtcg agcccccctc atttttgatt tgcatcccac
     4801 gccaagccgg ttagtctggt agtttggact tatgacgggt gcccgagccg acgccgctgg
     4861 cgaacgccgc gcctgacatc tttagagctc cattctgttt cgccgccgag accggttcat
     4921 gtcgcctgaa cccaccgaac cgattcagta cgcccatttg cccgacaata tcatcgtcat
     4981 ccgggtgctg ggcaaaggca cccatctgca aagccccagc ctgcgctatg tcttcgatca
     5041 gacgcgcgac gccgcccccc cgccccgcta tatcatcgac ctggatcgct gcaccaccat
     5101 ggacagcacg ttcatgggga ccctcgcctc gatcggcctg catcaggtcg agcgcaccgg
     5161 ctcgcgcgtc atcgtcacca atattcagga gcatgtcctc cgcttgctca ataccctcgg
     5221 cctgaagtac atcctcgatc tgcgcgccaa tcacgaccaa accgtcaacc ggctcgggct
     5281 cggcgaggag gccttcaccc ccgccgccgg accccgcctc gacaagctgg atcggattgt
     5341 gctcatgatt gaagcgcacg aacgtctgat cgatgtcgac agccagaacg aggtcaagtt
     5401 tcagggggtc ctgcgaaccc tgcgccagtc gctcgatgaa gaacagcaga ggccgtcttg
     5461 aggggcgccg cttccccccc aatcacccat ccagataccc gcatcccgga gtagatgact
     5521 gaatgatccg ccgtcgcacg ttacccaatg gcgtccaggt cgtcaccgag cagctcgaca
     5581 ccgtccgctc gtgcgccatc ggcgtcttcc tgcaagtcgg cagccgccac gaacgccccg
     5641 ccgagaacgg catcagtcat ttcctcgagc acatgctgtt caagggaacg tcgcgccgca
     5701 ccgcccgcca gatcgccgac gccaccaact acctgggcgg caacctgaac gccttcacga
     5761 cccaggaatt gatctgcctc cacgccaaga cggtcgacag caaggccccc agcacgctcg
     5821 acctgttcgg tgaaatgctg atcgactcga tcttcccccc cgaggaaatc cgccgcgagc
     5881 gccaggtcgt cctcgaagaa ttcaaaatgt atgaggacac ccccgacgag ttgagcgtcg
     5941 atctcttcct gcgcaacctc tggccggatc acccgctggg ccgtccggtg atcggttccc
     6001 gcacctcgat cagccgcctc tcgcgcgccg gcatccagcg gttcctgcgc cgcgtgttcc
     6061 atcccagccg gctgctgatc gtgctgtcgg gctcgttcga cagccgcgcg tgctcgcgcg
     6121 cgatcaatca gtggttcggc aaagtcgagc ccgccggcgc cgccgcgggt cgcctcggcg
     6181 gccggccgcc gcgcgccccc cgctcgcggc ggacccatct gcgccgccgc atcgaacaga
     6241 cgcatttctg cctcggcgtc cccgcgcccg accgcacctc gcccgatcgc ttcgccttca
     6301 gcctgctgaa catgatcctc ggcggcggga tgagctcgcg cctgtttcag gaaattcgcg
     6361 aaaagcgcgg cctggcctac tcgatcggct cgttcaccca gtccttcacc gacgccggct
     6421 gtttcgccgt cagcggcggc accagccccg atacctacgg cgaggtgctg cgcatcacgc
     6481 tggaggagtt gcagcgcatc accgaggaac ccgcccccga ggaggaactc gtgatggccc
     6541 gcgagcaact ggtcgattcc ctcctgatga gcctggaaaa taccgagtcg cgcatgctcc
     6601 gcctcgccga cagcctcctc aatttcggcc gggtcgtgcc cgtcgatgaa atcctgaccc
     6661 aactgcggcg ggtcgagccg gacgacgtcc gccggatcgc ggccaagtat ctcaccggcg
     6721 cctcgctcgc ccttgcgtcg atcggcccca atgctaagat ggagcccatt tcggaaaacc
     6781 ttcggcaggt tgccgcctga gagatgcccc ccgccatgaa ttccgccatc cacattttcc
     6841 atcgcttcac gggggtgggg attgccggtt tgatcctggc cgcccacctg gcgttcgtcg
     6901 ccggctgcca gcgcgtccgg accgacgccg cgttgccgaa cctcgtgcag gtcagcgccg
     6961 aaggctatgt cgatccggca acagtggccc cgcgcgccgc tgcggtcgcc ggcgacgatc
     7021 tgacctcggc cgccgtcgtc gtccatgacg gctcgatcac cgatcccggc cagcgcgtcc
     7081 tcgcggcgcg cgccgcggcg cggcgtcagg ccctgcgcgg catcggcgag cagcttcagc
     7141 atctgcgggc cgcggatggg cgcccgctcc tgctcacgct gcgcgccgcc gattcacccg
     7201 cgattgcgca actcaatgag ttgctcgatc gccacgccga gacgacctac accgagttca
     7261 acggccgctc caaggcgctc gtcctgctcc ccgcggcgcg actcaatgaa ttcctcgcgg
     7321 ccgccggcgg cgcgcccgaa tcctccagtc tgccgctcac cgccgaggag gaccaggtcc
     7381 tgcgcgcgca ggcctacgag gccgcgctgc gcgacgcgcg caagcagctc cgcgacgtca
     7441 tcccgatcgt tccgatcgag cccaacggcc cgacgatcgg cgaagtcatc gagcggcggc
     7501 cctcgctcgt caacgagctc aacgccctga tgctggccac gcagcccgat acggttgaat
     7561 accccgaacc gggccgctgc cggatgaccc tctccttcga catgggccag gcgcgcgagc
     7621 tggcgcgcgc ggcgttccgg cagaaggact gaccgcccgt gccccagcct gagatgatcc
     7681 cgtcttccga ctcgccgcgc atcgttctgg cctcggcctc cccccgccgc caggcgctgc
     7741 tcaaggccgg cggcgtccgt ttcgaggtcc gcgtctcggc cctcgatgaa tcgccctacc
     7801 atcacccctc cgcgccgcgc gccagtgcgc tcaagaccgc catggccaag gcgtcggccg
     7861 tcgcccccaa tctcccccgc gacacgatcg tg
//
LOCUS       DAOPCO010000186         7890 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8398_length_7890_cov_5.387620, whole genome shotgun sequence.
ACCESSION   DAOPCO010000186 DAOPCO010000000
VERSION     DAOPCO010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8398_length_7890_cov_5.387620"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1739
                     /locus_tag="PLS90_12145"
     CDS             <1..1739
                     /locus_tag="PLS90_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03197.1"
                     /translation="RLRFALESYSASSVPRDILYMYNLLGDPALDLQLTQRWGELEAR
                     PNPITPDIERVTVALNEVQPPSGKACLWLENPLGERIWESEPFTYGDGQIEREIELPA
                     ALREESPVDGNPHLVLYGWNAAEGRDFSASVDLVLARPHVELVDPRAEWLEGGRARIA
                     ATLRNASQVVIGPVDVRATRLASGPAAAVGETSLTISADDSREISFEFDEPGSTEPIA
                     YQLTLSSPWPADDPSIAVEPTTRVVLPPATDWSGFLTALSSRQFQGDAWRAQVVIGRG
                     GGAPAPRRLVLRHGDRPTSWALNFSPAPGWERAEQTLTVPGDIETATLELFAEDALSS
                     APLDVLPLARIPIEQPRLRIVPGSIRHRPENPTDGETIFIAFEVENAGNALSRPAQPA
                     LLDAPPEERGGVLPSQTGLPPATIPPLGAGRRMPVELRWDPIKNEGMQRIWVDLRAAR
                     GNEPKSQEQVAPYSLYVRAKSRLVRKRAPWIEQTAEDRMARRVKLNVEIANEGETDAR
                     NVLVSFFHGEEKTDENLLGVVEIERVPAERSAIAQLVWEYGDEDLDAQGKFKEGFSTE
                     IRLKGSAQRTAE"
     gene            complement(1868..2584)
                     /locus_tag="PLS90_12150"
     CDS             complement(1868..2584)
                     /locus_tag="PLS90_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK03198.1"
                     /translation="MLSFRLLGILLLAAGLILPTGCETMSEHKVASGAAIGAATGAVA
                     GGIIGHQSGNKETGALIGAGVGALAGAGIGYALDRQADDFDKIEEVEVERHPAEDYKE
                     EDYEEGGAYEEEEPPVEHLTLRISSEVLFDKDSATLTPRGKQKVAEVAAVLLEYPDSD
                     VLVKGYASSEGAEAYNLELSERRAKAVQNELIANRVSASRIQAIGFGEADPIASNETE
                     AGRALNRRVEIDVYPRGEVQ"
     gene            complement(2926..3915)
                     /locus_tag="PLS90_12155"
     CDS             complement(2926..3915)
                     /locus_tag="PLS90_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HPK03199.1"
                     /translation="MSPVAPVLTVTLNPAIDLNLVVDDWHTGEYVKARAVHRAAGGKG
                     INVSRVLAELGVASCAACLVGGADGEVFLSLLQGAPFDVVPIRLAGADGAADLSRATR
                     TNVTFTSARRARQIKVNQPGPRVGRAAWPAIEQFLTRQAIGRRWVVLSGSLPPGVPVG
                     AYGRLVRAAHRVGARVALDCGGAPLRAALSARPDLIKPNLDELTEASGGPCRSVREIV
                     AAARALLALGAGCVVVSRGGRSCLAVTPRGVLAGAPPPIRPRSPMGAGDSLVAGLVAA
                     LLKDPDKFDEALRLGIACGAACACEPDTVLARGPVIRRLLRRVRIMMPRVEAS"
     gene            complement(3912..5150)
                     /locus_tag="PLS90_12160"
     CDS             complement(3912..5150)
                     /locus_tag="PLS90_12160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03200.1"
                     /translation="MPVFPRSPRQLCWLFLLIPALTGCANGPKAKRAQFQTREQAFLL
                     IDEAKKMEVEGMPLLALERYNRAATLYNSPRAYYEMGQLFESEDKLDRAAVAYSEALR
                     LAPDYQEAKFAMLAIGYQPPDYVPTDEDLVLANEWIAAHRAEIERLRALDQPTTGPLQ
                     VAMTEEERMEQRRELIAAAAEQRQPTQAEVRAVLFEPQGSRRRLPSATDPVYPSTEDI
                     VLGTYPYHMQKAGQLERRQQFEDAAIEYERALQADPTQIEPRLSIGDMMMRLEHHPRA
                     RFHYERALEEFPESPQPNLKLGNYFLALNQPDRALEQYRIALDKDPFYAEAYNNIAFI
                     HMQQGQYDEAAAMFDQIKEMDPQYANAYLNRGIIASDVQNDPQTALDNFRRYIELNGS
                     RAAEVSRWVRELESELTPAQ"
     gene            complement(5150..5716)
                     /locus_tag="PLS90_12165"
     CDS             complement(5150..5716)
                     /locus_tag="PLS90_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03201.1"
                     /translation="MAAIGKTCWNGGALLALTLIGAAALAGCARQPRLPFRYDAEGFP
                     VQDKRFERHVQRDFFHHFSRTVYDPVTPPPYGYQGDKQTVYEQWGLPDYERREFKSIN
                     GEEVDEWLYLPENRIFQFIGDVMVYDGPLTDYEQILLRRGYPSRCEVQMTDTGTRIDV
                     FVYDGVFQPMLEEYHFQNGYLTQFQEGR"
     gene            5848..7260
                     /locus_tag="PLS90_12170"
     CDS             5848..7260
                     /locus_tag="PLS90_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPK03202.1"
                     /translation="MVTSPQRLFTDLAGAYDWLNSHINYERELGRIAYNNRVFELNGF
                     LRRIEALGRPQLGLRTIHIAGTRGKGSAALMLEALLRASGLRVATYTSPHIREYRERI
                     RIDGEVIAPELFVRCLQRIAPLDENGAATGLQFKTVFEHLTALFFLAARATAVDWAVV
                     ETGLGGRLDATNVVEPGPVLLTRIGLEHTHLLGRTIPAIAGEKAAILKPGGWGVVSAQ
                     AESEGAMDIFAQRARRTGARLDRAADLCPIRRVTPDASGLRVVVEFEGELLTVGLPLY
                     GPCQAENLQNALAMLSRLREEGLIARAPREALATALASVSIPGRMERWPTAPPLIIDA
                     AHCPTGADALVQALEAHFPGTRTHLLVGMMADKEHDGFFAALARWPGWRRVVCYPVDS
                     PRGASAADLADCARRHFPDVTLCTDLAAGLELALRIADKKDQIVAAGTVVSIAFLQDW
                     AATRLTMDVQQDGPSETQSR"
     gene            7232..7576
                     /locus_tag="PLS90_12175"
     CDS             7232..7576
                     /locus_tag="PLS90_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03203.1"
                     /translation="MGRAKLSRAELLEVLERNVQLYREEEAFVPYLVELAVYLLEYEY
                     DWSQSGGHPANRDMKPVDPPSFSRIQRQGDSFGMALSLQTNRRNRRRRCPHCGVEVGD
                     EIVCPSCRNMTR"
     gene            complement(7631..>7890)
                     /locus_tag="PLS90_12180"
     CDS             complement(7631..>7890)
                     /locus_tag="PLS90_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK03204.1"
                     /translation="ILDEPTTGLDPLTCQVINNLIRQTQERLHVTSIVVTHDLRSAQE
                     VGDRCSFLHEGRILETTRPRDLARSEHEIIRTFLEDAVVFT"
BASE COUNT         1274 a         2572 c         2663 g         1381 t
ORIGIN      
        1 ctcgcttgcg cttcgctctc gaatcctaca gcgccagttc agtgccgcgc gacatcctct
       61 acatgtacaa tctcctcggc gaccccgcgc tggatcttca actgacccag cggtggggcg
      121 aactggaagc gcgacccaac cccatcacgc ccgatatcga acgggtgacc gttgcgctca
      181 atgaagttca acccccgtca ggcaaagcgt gcctgtggct ggagaacccc ctgggcgagc
      241 gcatttggga aagtgagccg tttacctacg gcgacgggca gattgagcgg gagatcgaac
      301 tgcccgccgc gctgcgcgag gagtccccgg tcgacggcaa tccgcatctg gtcctctacg
      361 gatggaacgc ggcggagggt cgggatttct cggcctccgt ggacctggtg ctcgcgcggc
      421 cgcatgtgga actggtcgat ccgcgcgcgg aatggcttga aggcggccgg gcgcgaatcg
      481 ccgcgacgct gcgcaatgcc agccaggtcg tgatcggtcc ggtcgacgtc cgggcgacgc
      541 gactggcgag cggaccagcc gcggccgtgg gcgagaccag cctgacgatc tccgccgacg
      601 actcgcgaga gatcagtttt gaattcgacg aaccgggatc gactgagccg attgcgtatc
      661 agctcacgct ctcgtccccc tggccggccg atgacccatc gattgccgtg gagccaacaa
      721 cgcgggtcgt gttgccgccg gcgaccgact ggagcgggtt cctgacagcg ctcagctcac
      781 gccagttcca gggcgatgcg tggcgcgccc aggtggtgat tggccgcggc ggcggggcgc
      841 cggccccgcg ccggctggtc ttgcgccatg gcgaccggcc gaccagttgg gcgctcaatt
      901 tttcgccggc ccccggctgg gaacgggccg agcagacgct gaccgtgccg ggcgacatcg
      961 agaccgcgac gctggagctc tttgcggagg atgcgctctc gagcgcgccg ctggatgtcc
     1021 tgcctttggc gcgtatcccg atcgagcaac cccgcctgcg catcgttccc ggcagcatcc
     1081 gccaccggcc ggagaatccc accgacggcg agactatttt catcgcgttc gaggtcgaaa
     1141 acgcgggcaa cgcgctttcg cgcccggcgc aaccggcgct gctcgacgcg cccccggagg
     1201 aacgcggcgg cgtgcttccc tcgcagaccg gactcccgcc ggccaccatc ccgccgctcg
     1261 gcgcgggccg ccgcatgccg gttgaattgc gctgggaccc gatcaagaac gaagggatgc
     1321 agcgcatctg ggtggatctg cgcgccgcgc gcggcaacga gccaaagtcc caggagcagg
     1381 tcgcgcctta ttcgctgtat gttcgcgcca agagccggct ggtgcgcaaa cgggcgccgt
     1441 ggatcgagca gacggccgag gatcgcatgg cccggcgcgt caaactcaat gtagagatcg
     1501 cgaacgaggg tgaaaccgac gcgcgcaacg tcctcgtctc gttcttccac ggcgaggaga
     1561 agaccgacga gaacctgctg ggggttgtgg agatcgagcg ggtgccggcc gaacggagcg
     1621 ccatcgcgca attggtctgg gaatatgggg acgaagacct ggacgcgcag ggaaagttca
     1681 aggagggctt ctcgaccgag attcgcctga agggctccgc gcaacggacc gcggagtgat
     1741 cggattgagc gggtgggggc gtctccgggt cgcaatttcc tgtcattaaa tgaaggtctc
     1801 ggatccgatt cggatccgag accttgatcg ttccaggcga tgctttgcat gcagaggctg
     1861 gttcgctcta ctgcacttcc ccgcgcggat aaacgtcgat ctcaacgcgg cggttgagcg
     1921 cgcgaccggc ttcggtctcg ttgctcgcga tcggatcggc ctcgccaaac ccgatggcct
     1981 gaatccggct ggcgctgacg cggttggcga tcagctcgtt ctgcacggcc ttggcccggc
     2041 gttcggagag ttccaggtta tacgcttcgg cgccctcgga actggcatac cccttcacca
     2101 ggacatccga atcggggtat tcgagcagga ccgccgccac ttcggcgacc ttctgcttgc
     2161 cgcgcggcgt caaggtggcc gaatccttat cgaagaggac ttcgctgctg atgcgcagcg
     2221 tcaggtgttc gacgggcggc tcctcttcct cgtaagcgcc cccttcctcg taatcctctt
     2281 ccttgtaatc ctcggcggga tggcgctcga cttcaacctc ttcaattttg tcgaagtcgt
     2341 ccgcctggcg atccagcgcg taaccgatgc cggcgcccgc cagagcgccc acgccggcgc
     2401 cgatcagggc gcccgtctcc ttgttgccgc tctggtggcc gataatgccg ccggccacgg
     2461 cgccggtcgc cgcgccgatg gccgcgcccg aggcgacctt gtgctcgctc atcgtctcgc
     2521 agccggtcgg caggatcagt cccgccgcca aaagcagaat tcccagcagt ctgaatgaga
     2581 gcatgtgcat ctttccgttc ctcctgttca acgacggaga ccgcggcggg gcttcacgaa
     2641 cagcgggcct tccacagcgg acttgcgggg gtgacccgac gcgagaaccg ttccagtgtg
     2701 gcgttgcctt cggccgattc tcgaacatac atcggcgcgt cactctagcc ctaccgacac
     2761 cgtaagctca aggatgttcc gtgaagcgga agctgtcgct gggatcaagc cgcgaacccc
     2821 gtccctcgtc gctcgccctc gtgattgccc tcaaaggcca ctctgaattc ggttctcggc
     2881 tctcgtccct cgttcggctc tcgaaatgtc ggccccgtcg gtccctcaac ttgcttccac
     2941 cctcggcatc atgatcctca cgcggcgcag cagccggcga atcaccggcc cccgggccag
     3001 cacggtatcg ggctcgcagg cgcaggcggc gccgcaggcg atccccaggc gcagggcttc
     3061 gtcgaatttg tccggatcct tcaggagcgc cgccaccagc cccgccacca gcgaatcgcc
     3121 cgcgcccatc gggctgcgcg gccggatcgg cggcggcgcg cccgccagca ccccccgcgg
     3181 cgtgacggcg aggcacgacc ggccgccgcg cgagacgacg acacagccgg cgcccagcgc
     3241 gagcagcgcg cgcgcggccg caactatttc tcgtaccgat cgacacggtc ccccgctggc
     3301 ttcggtcagc tcgtcgagat tgggcttgat cagatcgggc cgcgcgctta gcgccgcgcg
     3361 cagcggcgcg ccgccgcagt cgagcgcgac gcgggccccg acacggtggg cggcgcggac
     3421 cagccggccg taggcaccga cgggaacgcc gggcggcagc gagccggaga gcaccaccca
     3481 ccgccggccg atggcctggc gggtcaggaa ttgctcaatc gcgggccatg ccgcgcggcc
     3541 cacgcggggt ccgggctggt tgaccttgat ctgccgcgcg cggcgcgccg aggtgaaggt
     3601 gacgttggtg cgcgtggcgc gcgacaggtc ggcggccccg tcggcgccgg ccagccgaat
     3661 cggcacgacg tcaaacggcg cgccctgcag cagcgaaagg aagacctcgc catcggcgcc
     3721 gccgaccagg caggcggcgc aagaagccac gcccagttcg gccagcacac gcgagacgtt
     3781 gatccccttg ccgccggcgg cgcgatgcac ggcgcgcgcc ttgacgtatt cccccgtgtg
     3841 ccagtcgtcg acgacgagat tcagatcgat cgccggattg agcgtgacgg tcaggacggg
     3901 ggcgaccggg ctcattgcgc gggagtcagt tcgctttcca gttcgcggac ccagcggctg
     3961 acctcggccg cgcgcgaccc gttcagctcg atatagcggc ggaagttgtc cagggcggtc
     4021 tggggatcat tctgcacgtc gctggcgatg attccccggt tcagatacgc attggcgtac
     4081 tgcgggtcca tctccttgat ctggtcgaac atcgcggcag cctcgtcata ttgaccctgc
     4141 tgcatatgga tgaaagcgat gttgttgtag gcctcggcgt agaacggatc cttgtccagc
     4201 gcgatgcgat actgttccag cgcgcggtcg ggctgattca gcgcaaggaa gtagttgccg
     4261 agcttcaggt tgggctgggg cgactcggga aattcctcca acgcccgttc gtaatgaaac
     4321 cgcgcgcgcg ggtgatgctc caggcgcatc atcatgtcgc cgatgctcag gcgcggctcg
     4381 atctgcgtcg ggtcggcctg gagcgcccgc tcgtattcaa tcgcggcatc ctcgaactgc
     4441 tggcgccgct cgagctggcc ggccttctgc atgtggtagg ggtaggtgcc cagcacgatg
     4501 tcctcggtgc tggggtaaac cgggtcggtg gccgagggaa ggcgccgccg ggagccctgc
     4561 ggctcgaaca gcacggcgcg gacttcggcc tgggtcggct ggcgttgctc ggcggccgcg
     4621 gcaatcagct cgcgccgttg ctccatccgt tcctcttcgg tcatcgccac ctggagcggt
     4681 ccggtcgtcg gctgatccag ggcgcgcagg cgctcgattt ccgcgcggtg tgcggcgatc
     4741 cattcattgg ccagcaccag atcctcatcg gtcggcacgt aatcgggcgg ttggtagccg
     4801 atcgcgagca tcgcgaactt ggcttcctgg taatcggggg cgaggcgcag ggcttcggag
     4861 taggccacgg cggcgcgatc cagtttgtcc tcgctctcga acaactggcc catttcatag
     4921 taggcccggg ggctgttgta gagcgtggcg gcgcgattgt agcgctccag cgccagcagc
     4981 ggcatcccct cgacttccat cttcttggcc tcgtcgatca gcaggaaggc ctgttcgcgg
     5041 gtctggaatt gcgctcgttt ggccttgggc ccattggcgc agccggtcaa tgccggtatc
     5101 agcaggaaaa gccaacagag ctgtcgcggg gagcggggga atacgggcat tagcggccct
     5161 cttggaactg ggtgaggtat ccgttctgga agtggtattc ttccaacatg ggttgaaaga
     5221 cgccatcgta gacgaaaacg tcgatgcgcg tgccggtgtc ggtcatctgg acctcgcacc
     5281 ggctggggta accgcggcgc agcaggatct gttcgtagtc ggtcagcgga ccatcataga
     5341 ccatgacgtc gccgatgaac tggaagatgc ggttttcggg cagatagagc cattcgtcca
     5401 cttcttcccc gttgatcgac ttgaactccc ggcgctcgta atcgggcagg ccccattgtt
     5461 catagaccgt ctgcttgtcg ccctggtagc cgtacggggg cggggtcacg ggatcataaa
     5521 cggtccggct gaaatggtga aagaaatcgc gctggacatg gcgttcgaag cgcttgtcct
     5581 gcacgggaaa tccctcggcg tcgtagcgga aggggagccg gggctggcgg gcgcaaccgg
     5641 ccaacgcggc cgcgccgatc agcgtcagcg cgagcagggc gccaccgttc caacaggtct
     5701 tgccgatcgc ggccatgacg ggtcttgcct tcggggcgga ttgccgtaga ataggacgat
     5761 tacgcgcgcc aacggggtgt ttggacgcgc atcctgcatc actattcggg aaggttcacc
     5821 tcctgtcaag ggtgttcgcc cctgcccatg gtcacatcgc cgcaacggct ttttacagat
     5881 ctggcggggg cctatgactg gctcaacagc catattaatt acgagcgcga gctggggcgc
     5941 atcgcctaca acaaccgcgt ttttgagctg aatggctttc tccgccgcat cgaggcgctg
     6001 gggcgcccgc agctcggtct gcgcacgatt catatcgcgg ggacgcgcgg caagggctcg
     6061 gcggcgttga tgctcgaggc gctgctgcgc gcctcgggtc tgcgcgtggc aacttacacc
     6121 tcgccgcaca tccgcgaata ccgcgagcgg atccggatcg acggcgaggt gatcgccccc
     6181 gagctgttcg tccgctgcct gcaacgcatc gcgccgctgg atgaaaacgg cgcggccacg
     6241 ggtttgcagt tcaagacggt gtttgaacat ctgaccgccc tgttctttct ggcggcgcgc
     6301 gcgacggccg tcgactgggc ggtcgtcgag accggcctgg ggggccgcct cgacgcgacc
     6361 aacgtagttg agcccgggcc ggtcctgctg acgcggatcg gactggagca cacgcatctg
     6421 ctgggccgga ccatcccggc gatcgccggc gagaaggccg ccatcctgaa gccgggcggg
     6481 tggggggttg tctccgcaca ggccgagtcg gagggggcga tggacatctt cgcccagcgg
     6541 gcccgccgga ccggcgcccg gctggatcgg gccgctgacc tctgcccgat ccggcgggtg
     6601 acgcccgatg cttcgggact gcgggtggtc gtggagttcg agggtgaact gctgacggtc
     6661 gggctgccgc tctacggccc gtgccaggcc gagaatctcc agaatgcact ggcgatgctg
     6721 agccggctgc gcgaggaggg cttgatcgcc cgcgctccgc gcgaagcgct ggccacggcc
     6781 ctggcgtcgg tcagcatccc gggacggatg gaacgctggc cgacggcgcc gccgctgatc
     6841 atcgacgcgg cccactgccc gacgggcgcc gacgccctgg tccaggcgct ggaagcccat
     6901 ttcccgggaa cgcggacgca tctgctggtc ggcatgatgg ccgataagga acatgacggg
     6961 ttcttcgcgg cgctggcccg ctggcccggt tggaggcggg tggtctgtta tccggtggac
     7021 agcccgcgcg gggcgtcggc cgcggacctc gccgactgcg cccggcgcca ttttcccgac
     7081 gtgacgttgt gcacggattt ggccgcggga cttgagttgg cgctgcggat tgccgacaag
     7141 aaggatcaga tcgtggccgc cggcacggtt gtgtccattg ctttcctgca ggactgggct
     7201 gcgacgcgac tcaccatgga tgtgcagcaa gatgggccga gcgaaactca gtcgcgctga
     7261 actgctggaa gtgctcgaac gcaatgtcca gttataccgg gaagaagaag cgtttgttcc
     7321 gtatctggtg gaattggcgg tttaccttct cgagtacgaa tacgactggt cgcagtcggg
     7381 aggccatccg gccaaccgcg acatgaagcc ggtcgacccc ccgtcgttct cgcgaatcca
     7441 gcgccagggc gattcgttcg gcatggcgct cagcctgcag accaaccggc gcaatcggcg
     7501 gcgccgctgt ccccactgcg gtgtcgaagt cggcgacgag atcgtctgcc cctcctgccg
     7561 caacatgacg cgctgaccgg ccgtccggca gccgcggaca cccgaagctt tcggtccgtg
     7621 tccgggcacc ctacgtaaag acaacggcgt cctcgaggaa cgtgcggatg atctcgtgct
     7681 cggaacgcgc gaggtcgcgc ggacgggtcg tctccagaat gcgcccttca tgcagaaacg
     7741 agcagcggtc gccgacctcc tgcgccgagc gcaggtcgtg cgtcaccacg atcgacgtaa
     7801 cgtgcaggcg ctcctgcgtt tgtcgaatca aattattaat cacctgacag gtcaggggat
     7861 ccaggccggt cgtcggctca tcgagaatat
//
LOCUS       DAOPCO010000187         7842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8452_length_7842_cov_4.545525, whole genome shotgun sequence.
ACCESSION   DAOPCO010000187 DAOPCO010000000
VERSION     DAOPCO010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7842
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8452_length_7842_cov_4.545525"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            653..6403
                     /locus_tag="PLS90_12185"
     CDS             653..6403
                     /locus_tag="PLS90_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPK03205.1"
                     /translation="MAAKTASPATPAGAHALNDRELLKVLIAFEKGDFSVRAPTDLTG
                     MPGKIADTLNLILEHNEHIATELQRMQRMVGNEGRLSQRASMNGARGQWSATIHSINT
                     LVDDLVRPINETSRVIGAVARGDLSQMMNMEVDGRELQGEFRRTAKIVNTMVDQLGSF
                     ASEVTRVAREVGTEGKLGGQAKVKGVAGTWKDLTDSVNTMAENLTGQVRNIAAVTTAV
                     AKGDLSKKITVDVKGEILELKDTINTMVDQLGSFASEVTRVAREVGVEGKLGAQAEVK
                     GVSGTWKDLTDSVNTMAENLTGQVRNIAAVTTAVARGDLSKKITVDVKGEILELKNTI
                     NTMVEQLRSFASEVTRVAREVGTEGKLGGQAKVAGVSGTWKDLTDNVNGLAANLTGQV
                     RNIAEVTTAVARGDLSKKITVDVKGEILELKDTINTMVDQLSTFASEVTRVAREVGTE
                     GKLGGQARVPGVSGTWKDLTDNVNGLAANLTGQVRNIAEVTTAVARGDLSKKITVDVK
                     GEILELKDTINTMVDQLSTFASEVTRVAREVGTEGKLGGQARVQGVAGTWKDLTDNVN
                     SMAGNLTSQVRNIADVTTAVANGDLSKKITVDVKGEILELKDTINTMVDQLRSFASEV
                     TRVAREVGTEGKLGGQAYVPGVAGTWKDLTDNVNSMAGNLTSQVRNIADVTTAVANGD
                     LSRKITVDVKGEILELKDTINTMVDQLSSFASEVTRVARDVGTEGKLGGQAYVPGVAG
                     TWKDLTDNVNSMAGNLTSQVRNIADVTTAVANGDLSRKITVDVKGEILELKDTINTMV
                     DQLSSFASEVTRVARDVGTEGKLGGQARVQGVAGTWKDLTDNVNSMAGNLTNQVRGIA
                     RVVSAVAQGDLKQELVLTARGEIAELGDTINAMIRTLATFADQVTSVAREVGVEGKLG
                     GQALVPGASGTWKDLTDNVNQLAANLTTQVRAIAEVATAVTKGDLTRSIRVDARGEVA
                     SLKDNINQMILNLRETTQKNTEQDWLKTNLAKFTRMLQGQKDLMAVARLILSELAPVV
                     SAQQGVFYVLEQDDDEDRPYLNLLASYAFRERKHLSNRFYLGEGLVGQSALEKQPILL
                     RNVPGDYIRINSGLGDSPPLNIIVIPVLFEGQVKAVMELASFEPFNPMHHVFLEQLSD
                     SIGIVLNTIEANMRTESLLEQSQSLAKELQHGQLELQKTNDELEEKARLLAEQNAEVE
                     KKNREVQLAKEALEEKAEQLALTSKYKSEFLANMSHELRSPLNSLLILSRQLADNGSG
                     NLDPKQVEFAETIHASGSDLLALINDILDLSKIESGIVSLELEDVNFRDIQHFVERNF
                     RQIANMKKLLFAVELEPGLPEAVTTDEKRLFQVLKNLLSNAFKFTEQGGVELRIAPAR
                     EGWNPTQADLDPDQGVIAFSITDTGIGIAPDKQRVIFESFQQAEGSTNRKYGGTGLGL
                     SISREISRLLGGDITLESELGQGSTFTFYLPVQFAEGRPGGREHRSASSAPGEGEPPA
                     DGDGAARRRQLEKVPLMVETSILDDRTRIQPGDRVMLIIEDDRQFAQTLLDIAHEQDF
                     KALIATRGENVFGLVVNFTPDAVMLDIILPDIDGWKILGRLKNNPVTRHIPVLVVTID
                     DDRPRAFKRGAFDVLRKPVAREQIVRSVAKMRQFIERPVKRLLLVEDEEQEREQIREL
                     IGNGDVKIEAIGSVEEALDFLRSESPDCMVLDLLLPGTSGFELVERIEQENLAPAMPI
                     IVYTGKELTREEEQRLGRMVQTVIAKDVRSPERLVDEVSLFLHRRTDALPEEKQTIVK
                     RIRESDEILRGRRVLVIDDDVRNIFALASVMEQHGIKAIPAESGREGLAVLRDGPPVD
                     LVLIDIMMPEMDGYETIRRIRAETRFKSLPIVAVTAKAMKGDRENCIEAGASDYVPKP
                     VDMDYLFSMLRELLCR"
     gene            6400..>7842
                     /locus_tag="PLS90_12190"
     CDS             6400..>7842
                     /locus_tag="PLS90_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03206.1"
                     /translation="MTAMDEEKVNVLLVDDRPENLRALEVVLEPLNENLVRVNSGEAA
                     LRAALRSEFAVILLDVRMPDLDGYETASMIRERPGSAHTPIIFVTAEDADRRRMASGY
                     AAGAVDYIVKPIEPGILRSKVRVFADLYRNAREVERQARELEAQNLQQRELIARNAEL
                     QQRLTARAVEADRRAQQLKIMSSELAHAEQRERRRLARILHDHLQQILVASLFRLQSL
                     RRGSCHDLAGGLSDLRELLQDALQASRSLTAELSPPILHEAGLEAALQWLGRRLRKEL
                     GLAIELDVRLNQVQLPDELNVLLFQAVRELLFNVHKHSGTNQAFLTACADGDMLHVEV
                     ADQGRGFDPALLDLDHTTHESFGLFSIRERLEVVGGGFYVHSRPGGGACFRLELPKSK
                     CLQRQPRAAAGAPAAREESLLPEPPVESPARARTRVLVADDHKILREGLANLLREKPG
                     LEVVGEAASGEAAVDLARALRPDVVIMDISM"
BASE COUNT         1527 a         2459 c         2665 g         1191 t
ORIGIN      
        1 gcaagtcatt cctttcgctg aggtgttcgc gaaatggggg gttaaccgtt gatgcctgct
       61 ttcggagtgt ccgggacgct tttcgaaagc ggcgcaaggc gattcgtagc gagttggcgg
      121 cctgcgtcag aggggtgagg gcgcggttgg gccggcggct gggcgtgcgc catcgcttgg
      181 gccggccggc cgcggaggaa tggccgcaga tcggacccag ccgacgccga tcgcgcccca
      241 gcgccacgca cagcctgcgg ccgtggtccc ctggcgcgcg cccccaggcg cttcggcagg
      301 gtccgcggtt gcggcagtcg atgccgaagg ccgcccgcgg gcagcgaaac ttgtgcgtcc
      361 agccggctgg tgggtatgcg ttttgctaaa atcacgattc tttggtgcat cgcatgaatc
      421 ccgatccgat tatcccgtgg cgcggccaac agccggcgcg gtggcgctcc gctccgacac
      481 gccgcgtgcc gattcaaccg ggtcctccat cccctgccgc agctcggccg ccgcaacttt
      541 cccgtggggt taccgggtga gtgcttgacc cagaaggtcc aacccgcaac ccgccacctt
      601 cagcggcccc aacccagccc gcatcgaaac tttgagccgg agataacgcc ccatggccgc
      661 caagaccgcc tcccccgcca cgcccgccgg ggcccatgcc ctgaatgacc gggagctgct
      721 caaggtcctg atcgcgtttg aaaaggggga cttctccgtc cgcgcgccga ccgatctgac
      781 cggcatgccg ggcaagatcg ccgatacgct gaatttgatt ctcgaacata acgaacacat
      841 cgcgaccgaa ttgcagcgga tgcagcgaat ggtgggcaac gaagggcggc tgagccagcg
      901 ggcctcgatg aacggcgcgc gcggccagtg gagcgcgacg atccattcga tcaacacgct
      961 ggtcgatgac ctggtgcggc cgatcaacga gacgtcgcgc gtcatcggcg cggtcgctcg
     1021 gggcgatctg tcgcagatga tgaacatgga ggtggatgga cgcgaactgc agggggaatt
     1081 ccgccggacg gccaagatcg tcaacacgat ggtggatcag ctcggcagtt tcgccagcga
     1141 ggtgacgcgc gtggcgcgcg aagtcggcac cgagggcaag ctgggcggcc aggccaaggt
     1201 caagggggtg gcgggaacct ggaaggacct gaccgacagc gtcaacacga tggctgaaaa
     1261 tttgaccggc caggtgcgca atattgcagc ggtcaccacg gcggtggcca agggcgatct
     1321 gtcgaagaaa atcaccgtcg acgtcaaggg ggagattctc gagctgaagg acacgatcaa
     1381 cacgatggtc gaccagctcg gcagtttcgc cagcgaggtg acgcgcgtgg cgcgcgaggt
     1441 cggcgtcgaa ggcaagctgg gcgcccaggc cgaggtcaag ggggtctcgg ggacgtggaa
     1501 ggacctgacc gacagcgtca acacgatggc cgagaacctg accggccagg tgcgcaacat
     1561 tgccgccgtg accacggcgg tcgcccgcgg cgatctgtcg aaaaagatca cggtcgacgt
     1621 caagggggag atccttgagt tgaagaacac catcaacacg atggttgagc agctccgctc
     1681 gttcgccagc gaagtgacgc gcgtggcgcg cgaggtcggc accgagggca agctgggcgg
     1741 tcaggccaag gtcgccggcg tcagcggcac atggaaggac ttgaccgaca acgtcaacgg
     1801 cctggcggcg aacctgaccg gccaggtgcg caacatcgcc gaagtgacga cggcggtggc
     1861 gcgcggcgac ctttccaaga agatcaccgt cgacgtcaag ggggagatcc tggagctgaa
     1921 ggacacgatc aacacgatgg tcgatcagct ttcgacgttc gccagcgagg tgacgcgcgt
     1981 ggcgcgcgag gtcggcaccg aaggcaagct gggcggtcag gcccgcgtgc cgggcgtcag
     2041 cggcacgtgg aaggacctga ccgacaacgt caacggcctg gcggcgaacc tgaccggcca
     2101 ggtgcgcaac atcgccgaag tgacgacggc ggtggcgcgc ggcgaccttt ccaagaagat
     2161 caccgtcgac gtcaaggggg agatcctgga gctgaaggac acgatcaaca cgatggtcga
     2221 tcagctttcg acgttcgcca gcgaggtgac gcgcgtggcg cgcgaggtcg gcaccgaagg
     2281 caagctgggc ggtcaggccc gcgtgcaggg cgtggccggc acgtggaagg acctgaccga
     2341 caacgtcaat tcgatggccg gcaatctgac gagccaggtg cgcaacatcg ccgacgtgac
     2401 gaccgccgtc gccaacggcg acctctccaa gaagatcacg gtcgatgtga aaggcgaaat
     2461 tctcgagctg aaagatacga tcaacacgat ggtggatcag ttgcggagct tcgccagcga
     2521 ggtgacgcgc gtggcgcgcg aggtcggcac cgagggcaag ctgggcggcc aggcctatgt
     2581 gcccggcgtg gccggcacgt ggaaggatct gaccgacaat gtgaactcga tggccgggaa
     2641 cctgacgagc caggtgcgca acatcgccga tgtgacgacc gccgtggcca acggcgatct
     2701 ctcgcgcaag atcacggtcg acgtcaaagg cgagatcctg gagctgaagg acacgatcaa
     2761 cacgatggtc gatcagctca gcagcttcgc cagcgaggtg acgcgcgtgg cgcgcgacgt
     2821 gggcaccgag ggcaaactgg gcggccaggc ctatgtgccc ggcgtggccg gcacgtggaa
     2881 ggatctgacc gacaatgtga actcgatggc cgggaacctg acgagccagg tgcgcaacat
     2941 cgccgacgtg acgacggcgg tggccaacgg cgacctctcg cgcaagatca ccgtggacgt
     3001 caagggcgag atcctggaac tgaaggacac gatcaacacg atggtcgacc agctcagcag
     3061 tttcgccagc gaggtgacgc gcgtggcgcg cgacgtgggc accgagggca agctgggcgg
     3121 ccaggcgcgc gtccaggggg tggccggcac gtggaaggac ctgaccgaca acgtcaactc
     3181 gatggccggc aacttgacca accaggtgcg tggcattgcc cgcgtcgtgt cggcggtggc
     3241 ccagggcgac ctcaaacagg aactggtgct gacggcgcgc ggcgagatcg ccgaactggg
     3301 cgacacgatc aacgccatga tccgcacgct ggcgacgttc gccgaccagg tgaccagcgt
     3361 cgcgcgtgag gtcggcgtcg agggcaagct cggcggccag gccctcgtcc ccggcgccag
     3421 cggcacgtgg aaggacctga ccgacaacgt gaaccagctc gccgccaacc tgacgacgca
     3481 ggtgcgcgcc atcgccgaag tcgccacggc cgtgaccaag ggcgacctga cccgctcgat
     3541 ccgcgtcgat gcccgcggcg aggtcgcttc gctcaaggac aacatcaacc agatgatcct
     3601 gaacctgcgc gaaaccacgc agaaaaacac cgaacaggac tggctcaaga ccaacctcgc
     3661 caaattcacc cggatgctcc aggggcagaa ggatctgatg gccgtcgcgc gcctgatcct
     3721 ctccgagctg gcgccggtcg tctcggccca gcagggggtc ttttacgttc tggagcagga
     3781 tgacgacgag gaccggccgt atctcaacct gctggccagc tacgccttcc gcgagcgcaa
     3841 gcatctgtcc aatcggttct acctgggcga agggctggtc ggccagagcg cgctggaaaa
     3901 gcagccgatc ctgctgcgca atgttcccgg cgattacatc cgcatcaact cggggctggg
     3961 cgattcccct ccgctcaata tcatcgtcat ccccgtcctg ttcgaggggc aggtcaaggc
     4021 cgtgatggag ctggcgagct tcgagccgtt caatccgatg catcacgtct tcctcgaaca
     4081 gctctccgat tcgatcggca tcgtgctcaa cacgatcgaa gccaacatgc ggaccgagtc
     4141 gctgctcgaa cagtcgcagt cgctggccaa ggagctccag cacggtcagt tggaactgca
     4201 gaagacgaac gatgaactgg aggaaaaggc gcggctgctg gcggagcaga acgccgaagt
     4261 cgagaagaaa aaccgcgagg ttcagctggc gaaggaggcc ctggaggaaa aggccgagca
     4321 actggcgttg acttcgaaat acaagagcga gttcctggcc aacatgagtc acgagctgcg
     4381 ctcgccgctg aacagcctgc tgatcctctc gcgccagctt gccgacaacg gcagcggcaa
     4441 cctggacccg aaacaggtgg aattcgccga gacgatccac gcctcgggga gcgacctgct
     4501 ggcgttgatc aacgatatcc tcgacttgtc caagatcgag tcgggcatcg tttcgctgga
     4561 gctggaggac gtgaacttcc gcgacatcca gcactttgtt gaacgcaatt tccggcagat
     4621 cgccaacatg aaaaagctct tgttcgccgt cgagcttgaa ccgggcctgc ccgaagccgt
     4681 gaccaccgac gaaaagcgtc tgttccaggt gctcaagaac ctgctgtcga acgcattcaa
     4741 gtttaccgaa caggggggcg tcgagctccg catcgccccg gcccgcgaag gctggaaccc
     4801 cacccaggcc gacctggatc cggaccaggg ggtcatcgcc ttctcgatca ccgacaccgg
     4861 catcggcatc gcgcccgaca agcaacgggt gatctttgaa tcgttccagc aggcggaggg
     4921 atcgaccaac cgcaagtatg ggggaaccgg gctggggctc tcgatcagtc gcgagatctc
     4981 gcgcctgctg ggcggcgaca tcacgctgga gagtgaactg ggccagggga gcacattcac
     5041 gttttacctg ccagtccagt ttgccgaggg ccggccgggg gggcgcgagc atcgttcggc
     5101 gtcgagcgcc cctggggagg gggagccgcc ggcggacggg gacggtgcag cccgccgccg
     5161 ccagctcgaa aaggtgccgc tgatggtgga gaccagcatc ctggacgacc ggacgcgcat
     5221 tcagccgggc gaccgcgtga tgctgatcat cgaggatgat cgccagttcg cgcagactct
     5281 gctcgatatc gcgcatgagc aggatttcaa ggcgctgatc gcgacgcgcg gcgaaaacgt
     5341 cttcggcctg gtcgtcaatt tcacccccga cgccgtgatg ctcgatatca ttctgcccga
     5401 catcgacggc tggaagatcc tcgggcggct caagaacaac ccggtgacgc gccacatccc
     5461 cgtgctggtg gtgacgatcg acgacgaccg cccgcgggcc ttcaaacgcg gcgcgttcga
     5521 cgtgctgcgc aagccggtcg cccgcgagca gattgtgcgc agcgtcgcca agatgcgaca
     5581 gttcatcgag cgtcccgtta aacgcctgct gcttgtcgag gacgaagagc aggaacggga
     5641 gcagatccgc gagctgatcg gcaacggcga tgtgaaaatc gaggcgatcg gcagcgtcga
     5701 ggaagccctc gacttcctgc gcagcgaaag ccccgactgc atggtgctgg atctgctgct
     5761 gccgggcacg tccggcttcg aactggttga acggattgag caggagaacc tcgccccggc
     5821 gatgccgatc atcgtctaca ccggcaagga actgacgcgc gaggaggagc agcgcctggg
     5881 ccggatggtg cagacggtca tcgccaagga tgtccgctcg cccgagcgtc tggtcgatga
     5941 ggtctcgctc ttcctgcacc ggcgcaccga tgccctgccc gaggagaagc agaccattgt
     6001 gaagcgcatc cgcgagagcg acgagatcct gcgcgggcgc cgggtgctgg tgatcgacga
     6061 cgacgtgcgc aatatctttg ccctggccag cgtgatggag cagcacggga tcaaggccat
     6121 cccggccgaa agcggccggg aggggctcgc cgtcctgcgc gacggcccgc cggtcgacct
     6181 ggtcctgatc gacatcatga tgcccgagat ggacggctac gagacgatcc ggcggatccg
     6241 cgccgagacg cgattcaaat cgctgccgat tgtcgcggtg accgccaagg cgatgaaggg
     6301 cgatcgtgag aactgcatcg aggccggggc gtcggactac gtgcccaagc ccgtcgatat
     6361 ggactacctg ttcagcatgc tgcgcgaact cctgtgccga tgaccgcaat ggacgaggaa
     6421 aaagtcaacg ttctgctggt ggatgaccgc cccgaaaacc tgcgggcgct ggaggtcgtc
     6481 ctggagccgt tgaatgagaa cctcgtgcgg gtcaattcgg gggaggccgc gttgcgcgcg
     6541 gcgctgcgga gcgagttcgc cgtgatcctg ctcgacgtgc gcatgccgga tctggacggc
     6601 tacgaaaccg cttcgatgat ccgcgagcgg cccggcagcg cccacacgcc gatcatcttc
     6661 gtcacggcgg aggatgccga tcgccggcgg atggcgagcg gctacgccgc cggcgcggtg
     6721 gattacattg tcaaaccgat cgaaccggga attctccgct caaaggttcg ggttttcgcc
     6781 gatctgtacc gcaacgcgcg cgaggtggaa cgccaggcgc gcgaactgga agcgcagaac
     6841 ctgcaacagc gcgagttgat cgcgcgcaac gccgaactgc agcagcgcct gaccgcccgc
     6901 gcggtcgaag ccgaccgccg cgcgcaacag ctcaagatca tgtcgagcga actggcccac
     6961 gccgagcagc gcgagcgccg gcggctggcg cgcatcctgc acgatcacct gcagcagatc
     7021 ctcgtcgcct cgctctttcg cctgcagtcg ctgcggcgcg gttcatgcca cgatctggcc
     7081 ggcgggctga gcgatctgcg cgaactgttg caggacgcgc ttcaggcgtc gcgcagcctg
     7141 acggccgaac tcagcccccc gatcctgcac gaagcggggc tcgaagccgc gctgcaatgg
     7201 ctcggccggc gcctgcgcaa ggagctgggt ctggcgatcg agctcgacgt ccggctcaat
     7261 caggtgcaat tgcccgacga gttgaacgtg ctgctgttcc aggcggtgcg tgagctgctg
     7321 ttcaacgtgc acaagcattc cggaacgaat caggcctttc tgacggcctg cgccgacggc
     7381 gacatgttgc atgtcgaagt ggccgatcag ggccgcgggt tcgaccccgc gctgctggat
     7441 ctcgaccata cgacgcatga atccttcggt ctgttcagca tccgcgagcg gttggaggtt
     7501 gtcggcggcg ggttttatgt ccacagccgg cccggcggcg gcgcctgctt ccggctcgaa
     7561 ctgcccaaat ccaagtgcct ccagcgccag cctcgggccg cggccggcgc cccggcggcg
     7621 cgcgaagaat ccctgttgcc cgagccgccg gtcgagtccc cggcgcgggc gcgcacccgc
     7681 gtgctggttg ccgacgatca caagatcctg cgcgaggggt tggccaatct gttgcgggag
     7741 aagcccggcc tggaggtcgt cggcgaagcg gccagcggcg aggccgctgt ggacctggcc
     7801 cgcgcgctgc gccccgacgt cgtcatcatg gacatctcga tg
//
LOCUS       DAOPCO010000188         7812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8481_length_7812_cov_4.816166, whole genome shotgun sequence.
ACCESSION   DAOPCO010000188 DAOPCO010000000
VERSION     DAOPCO010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8481_length_7812_cov_4.816166"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..2046)
                     /locus_tag="PLS90_12195"
     CDS             complement(511..2046)
                     /locus_tag="PLS90_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HPK03207.1"
                     /translation="MHPSAVSDSALNSLPDRPGARSLPLSITPAGDGLIWIEQTPGGR
                     VLSVAAPSASPLLADFQGDPQPAPEGLGLLHCPLTAPNAQALRAALPALQPRRLGLAT
                     SAGFGDRLGLATPGHVRALRETLARRPGRAIAPLFAQQSIREMTRTRRTPHAVLTDAT
                     FGALAAGWTGPVGADADHLKSPEDIDACLAAGFSMFTFDPGAHVDPAADTDSPAALEQ
                     KLAALPWAELESSPDDLRRRFADRLFDLGDRALRIDPLELARAAAKYGRAVAHVARLA
                     RHLDSRRPDYDLEISVDETETPTSPAEHLYLAVELARLGVRWVSLAPRFVGRFEKGVD
                     YLGDTRELAAQLSLHAAIARAVGAGPDRPYKLSLHSGSDKFSVYPLICAATGGLVHLK
                     TAGTSYLEALRVFTTAEPDLFREILDLGRACYDDDRRTYHVSARLDRVPAAADLNDAD
                     LPALLDDFDARQVLHVTYGSALDRFGAEMHQALRRHETAYEQTLARHFGRHLEPFAAH
                     AES"
     gene            complement(2276..3550)
                     /gene="sppA"
                     /locus_tag="PLS90_12200"
     CDS             complement(2276..3550)
                     /gene="sppA"
                     /locus_tag="PLS90_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPK03208.1"
                     /translation="MNEDQGTPPSGQFDPAGSQPSPLPGTGPTPPQGPSGPAPGASGA
                     PPAGAAGASPPPGAPGVPPRYGGGAPPRPVPPPYYQPYAQYAYPPHGAPPPKKKGGRG
                     WLVALVLLLLFGGGGALLLFILASVLGPSSSSSAGGGSILGGPRIGLVRIEGVIMQDA
                     QKEFWMESLRNLADDHRVRGIVLRIDSPGGSVGAAQEIYEYIIHLRSERGKPVYVSMG
                     DTCASGGYYIAAAADRIFSLKGTLTGSIGVIFTKPELSELADRLGYETEVVKSGRFKD
                     SGAMTRPMSPEERRIFEILIQDTYEQFIQDILAFRDDELRAVRQRMTPADFEALMLEP
                     PLIEGGEGLLRSVADGRAYSGQQAHELGLVDEIGTLDDVIAALARRLQITGRPRIEEP
                     RRELTFRDMLFARVDHILPRASSQLFFLMDRP"
     gene            complement(3677..4285)
                     /locus_tag="PLS90_12205"
     CDS             complement(3677..4285)
                     /locus_tag="PLS90_12205"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HPK03209.1"
                     /translation="MKRFIVGITGASGVIYGVRLVEALAADPAHEVHLIISPAGARVL
                     AEELDRRPRLSPFDPHSLFRLAEGAADRIRLHPFADIGAGPASGTFRADAMVIGPCSV
                     KTLGAVAAGLADNLITRAADVTLKEGRPLLLVPRETPHNLIHLRNMVSLAEAGAIILP
                     ASPAFYHRPATIDDLVRYVVQKILDRLGVAAADPIRWRDPAA"
     gene            complement(4282..5070)
                     /locus_tag="PLS90_12210"
     CDS             complement(4282..5070)
                     /locus_tag="PLS90_12210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013456935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPK03210.1"
                     /translation="MNPRFTTIPAPLDDPPPAPAADDQSPEKIRAMFGAITPTYDRLN
                     RLLSGRRDEVWRRRAARAALDGLQPCRAVADVATGTGDLACALLRELARRPGSSTAHV
                     VGLDFTRPMLAAARRKFSDARLAWAEADGLRLPLRSASVDAATIAFGLRNMADKPAAL
                     RELVRVVRPGGRVVILEFSQPPNPLFRALYDFYSFQVMPRLGHWLSRSDAYLYLGRSI
                     RRFWSPDELTRQMQAAGLTRIRAVPLMLGVVYLHVGERGEASAP"
     gene            5156..5791
                     /locus_tag="PLS90_12215"
     CDS             5156..5791
                     /locus_tag="PLS90_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03211.1"
                     /translation="MGGHEQPLPRHFRRRSSAVARWALFFALVACVLSASNLLLIWRD
                     GELVRNARLLTEEIGRQLDKRREARQSETPAAPTGERPADERSADTGGADQPAAGPLN
                     EALDNLSWRLERIGAMIRDKDARARLHLDVLRDDLESWRETTTAQGGEWIDRALTAVQ
                     SARRDFEQQAPQAADHLLTLAGQIRERMPAVGRLNPTREPMAPASEAGAEP"
     gene            5854..6363
                     /locus_tag="PLS90_12220"
     CDS             5854..6363
                     /locus_tag="PLS90_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPK03212.1"
                     /translation="MAETSFYRIGVLSDTHGYLHPALFDLFDGVQLILHAGDIGRDDV
                     LVELRTIAPVQAVAGNVDLPDPRARPLIRQLETPAGRIAMTHGHLAHASAHDHASLLG
                     QFAGFQPGIIIFGHSHLPLIEELDGVKLFNPGAAGRAGLTQHGATVGMISVEEGSGQV
                     RLEHLKLSA"
     gene            6525..>7812
                     /locus_tag="PLS90_12225"
     CDS             6525..>7812
                     /locus_tag="PLS90_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03213.1"
                     /translation="MCRPRFARRLTALLLMTGLLAPRGSAQDFDPPLGHAAEMIDGGT
                     VARVEIPTQAAELQTYPMPTEYPRQLLQEVPGRPFRPVDPPPQAFAWWKVPFYVALGL
                     PRDLVDGFVGGLAKVPIISPVFVFPAYELVPTQIVFRDPRDWHRWPAGPNRHDHGYYK
                     GNSWGWFPSAHMWKFHYTSERKLERNRAYNERLQAELRDRNREIETHNQLIENRKREA
                     RREALRAIEAGNGREAALRMIPYYQAYPLDEGAFALFATALAMYAPEGPEWVKPLLWY
                     ELNAAQPLPLTQAETQLALAREQHPERASLAEALIYVRLLLGEPRDALVVAAEHLTRE
                     PDDARRRRLVLETALSADDLATARQVYQALDSSTLETTDAALIRARMDLLAGDYRGAQ
                     QLLRIMHQEHPEDAYVSYYLGIAELQLALYEPAYPEG"
BASE COUNT         1280 a         2312 c         3110 g         1110 t
ORIGIN      
        1 tacgatcggg cctccttccg ggaggcaggc ggcgtcgggg ccacgggaca taggcgtccc
       61 atcttggccg cgccggccgg agaaggacaa gcgaaaaggc acggcgtatt ttcgagtggg
      121 aggggctgac aacggcttgg ctgagttgag caggaaaccg ttgaaacggt tgaaaagatt
      181 tatcgcgtcg ggtcaccggg ccgaggcccg gtgcaaatgt caaaaacccc ggggggccaa
      241 taaaagcccg ccggcgcggg ctctcgaagc ggctccccca ggggaaggag cttggaattg
      301 gccaggtgcg gtgatccaca aagctgtctc gaccccggtc cgtttgggga agtctcacgc
      361 ttccgcgtgt cgatggcgtc gctcccaggg atgccgtcgg tcccaggcgg tggttgcgct
      421 ggccgacggc ctgttttcga gagccgtgga cgagggacgg gagccgagag ccgaatttta
      481 gaagaccttt ggggggtgtg gacaagtggg tcaagattcg gcgtgggcgg cgaagggctc
      541 cagatggcgg ccgaaatggc gcgcgagggt ttgctcgtaa gccgtttcgt ggcggcgcag
      601 cgcctggtgc atttccgcgc cgaaacggtc gagcgccgag ccataggtca cgtgcaggac
      661 ctggcgggcg tcgaaatcgt ccagcagggc gggcaggtcg gcgtcgttca ggtcggccgc
      721 ggcggggacg cggtccagcc gggccgagac atggtaggtg cggcggtcgt cgtcgtagca
      781 ggcgcggccc aggtccagga tttcgcggaa aaggtcgggc tcggcggtgg tgaacacgcg
      841 cagcgcctcc aggtaactgg tgccggccgt cttgaggtgt acgaggccgc cggtcgcggc
      901 gcagatcagc gggtagacgc tgaatttgtc ggatcccgag tggaggctga gcttgtaggg
      961 ccggtcgggg ccggcgccga cggcgcgcgc gatggcggcg tgcaggctga gttgggcggc
     1021 cagttcgcgc gtgtcgccga ggtaatcgac gcccttttca aatcggccga cgaagcgcgg
     1081 ggccaggctg acccagcgca cgccgagccg ggccagttcg accgcgagat agagatgttc
     1141 ggcgggggag gtcggcgtct cggtctcatc gaccgaaatt tccagatcgt agtctggccg
     1201 gcggctgtcg aggtggcgcg cgaggcgggc gacgtgggcg acggcgcggc cgtatttggc
     1261 ggcggcgcgg gccagctcga gcgggtcgat gcgcagcgcg cgatcgccca gatcgaagag
     1321 gcggtcggcg aaccggcgcc gcaggtcgtc gggcgagctc tccagctcag cccaggggag
     1381 cgcggccagc ttctgctcca gcgcggcggg cgaatcggtg tcggcggcgg gatcgacatg
     1441 cgctcccggg tcgaaggtga acatcgagaa gccggcggcg aggcaggcgt cgatgtcctc
     1501 aggggatttg aggtggtcgg cgtcggcgcc gacgggtccg gtccagcccg ccgccagcgc
     1561 gccgaaggtg gcatcggtca agacggcgtg gggagtgcgg cgggtgcggg tcatttcgcg
     1621 gatcgattgc tgggcgaaga ggggggcgat cgcgcggccg gggcgccggg ccagggtttc
     1681 gcgcagggcg cgcacgtgac cgggcgtggc cagaccgagg cggtcgccga agccggcgga
     1741 ggtggcgagc ccgagccggc ggggctgaag ggcggggagc gcggcgcgca gggcctgggc
     1801 gttgggcgcg gtgagcgggc aatggagcag accgaggccc tcgggcgccg gctgggggtc
     1861 cccctggaaa tcagcgagca gggggctggc ggagggggcg gcgacggaga gaacgcgtcc
     1921 accgggggtc tgttcgatcc agatcaggcc gtcgccggcc ggcgtgatgg agagcggaag
     1981 ggaccgggcg cccgggcgat cgggcagtga attgagggcg gagtcggaga cggcggaggg
     2041 atgcacgaac gggttgaatc cttggcgaga gtggggttgc gggtcgtgtc gcgaacgggg
     2101 aaatgaggac gaacaaggag ttgtagcacg gagggggtcg gggtgtaaac gccaaacgta
     2161 aacgcttacg cgaacgcgca aggcccgaca gaatacgatg gggaaacagc ggggggtgtt
     2221 aagagcagag cataagggac gagggacgat tttttgaagg gacgagtttt tttcgtcagg
     2281 ggcggtccat gaggaagaac aactggctgc ttgcgcgggg gaggatgtga tcgacgcggg
     2341 caaagagcat gtcgcggaag gtcagctcgc ggcgcggctc ctcgatgcgg ggccgcccgg
     2401 tgatctggag ccgccgggcg agcgccgcga tcacgtcgtc gagagtgccg atttcatcga
     2461 cgaggcccag ttcgtgagcc tgctggccgc tgtaggcgcg gccgtcggcg accgaacgca
     2521 gcaagccctc cccgccctcg atcaggggtg gctccagcat cagcgcctcg aaatcggcgg
     2581 gggtcatgcg ctggcggacg gcgcgcagct cgtcgtcgcg gaacgcaagg atgtcttgaa
     2641 taaactgctc gtaagtgtcc tgaatcaaga tttcgaagat gcgccgttcc tcgggcgaca
     2701 taggacgggt catcgcgccg gaatccttga accggccgct cttgacgact tcggtctcgt
     2761 agccgaggcg atcagccagt tcgctcagtt ccggcttggt gaagatgacg ccgatcgagc
     2821 cggtgagggt ccccttgagg ctgaagatgc ggtcggcggc ggcggcgatg tagtagccac
     2881 ccgaggcgca ggtatcgccc atggagacgt aaaccggttt gccgcgctcg ctgcgcaggt
     2941 ggatgatgta ttcatagatt tcctgagcgg cgccgaccga tccgccgggc gaatcgatgc
     3001 gcaggacgat gccgcggacg cggtggtcgt cggccagatt gcgcagcgat tccatccaga
     3061 attccttctg ggcgtcctgc atgatgacgc cctcgatgcg gacgaggccg atgcgcgggc
     3121 cgccgaggat cgatccgccg cccgcggagg aggaggaaga ggggcccagg accgaggcaa
     3181 ggatgaagag cagcaacgcg ccgccgccgc cgaagaggag cagcagcacg agcgcaacga
     3241 gccagccgcg cccgcccttc tttttcgggg gcggggcgcc atggggcgga tatgcatact
     3301 gggcgtaagg ctgatagtag gggggcggga cgggccgcgg cggagcgccg ccgccataac
     3361 gcggcgggac gccgggcgcg ccgggcggcg gcgaggcccc ggcggcgccg gccggcggcg
     3421 cgccggaggc ccccggcgcc ggcccggatg gaccctgggg aggagtcggc ccggtgcccg
     3481 gcagcggaga gggttgcgag ccggcgggat cgaactggcc ggatgggggc gtgccttgat
     3541 cttcgttcat ctcaatctcc ctccgcccga tgcgccggcg atgcgcttgc ccggcggagt
     3601 ggggcggttc tgactccgtt atgcccgttt agcgcgcggg atgccaaagg aaatgcgcgg
     3661 cgggggggcg gaggtatcag gctgcggggt cgcgccagcg gatcgggtcg gcggcggcga
     3721 cgccgagccg atcgaggatc ttctgcacga cgtagcggac cagatcgtcg atcgtcgccg
     3781 ggcggtgata gaatgccggg ctggccggca ggatgatcgc gccggcctcg gcgagcgaaa
     3841 ccatgttgcg cagatggatc aggttgtgcg gtgtttcacg gggaaccagc agcagcgggc
     3901 ggccctcctt gagcgtcacg tcggcggcgc gggtgatgag gttgtcggcc agcccggcgg
     3961 cgacggcgcc gagcgtcttg accgagcagg ggccgatgac catcgcatcg gcgcggaacg
     4021 tgccgctggc cgggccggcg ccgatgtcgg cgaagggatg gaggcggatg cggtcggccg
     4081 cgccctcggc gagccgaaag agggaatgcg gatcgaacgg cgagagtctg ggccggcggt
     4141 cgagttcctc ggcgaggacg cgggcgccgg ccggggagat gatcaggtga acttcgtggg
     4201 cgggatcggc cgcgagcgct tcaaccaggc gcacgccgta gatgacgccg ctggcgccgg
     4261 tgatgccgac gatgaagcgc ttcatggggc gctcgcctcg ccccgctcgc cgacgtgcag
     4321 atagacgacg cccagcatga gcggaaccgc gcggatgcga gtcagcccgg cggcctgcat
     4381 ctggcgcgtc agttcgtcgg gcgaccagaa gcggcggatg gagcggccga ggtagaggta
     4441 ggcgtcggag cgcgaaagcc agtggccgag ccggggcatg acctggaacg aatagaagtc
     4501 gtagagcgcg cggaagaggg gattgggcgg ctgactgaat tcgaggatga cgacgcggcc
     4561 gccggggcgg acgacgcgga cgagctcgcg gagggcggcg ggtttgtcgg ccatgttgcg
     4621 caggccgaag gcgatcgtgg cggcatcgac cgaggcggag cgcagcggga ggcgcaggcc
     4681 gtcggcttcg gcccaggcca ggcgcgcgtc ggagaatttg cggcgggccg cggcgagcat
     4741 cgggcgggtg aaatcgaggc cgacgacgtg cgcggtagaa gaaccaggcc ggcgcgccag
     4801 ctcgcggagg agggcgcagg ccaggtcgcc ggtgccggtg gcgacgtcgg cgacggcgcg
     4861 gcagggctga aggccgtcga gcgcggcgcg ggcggcgcgg cggcgccaga cctcgtcgcg
     4921 ccggcccgag agcaggcggt tgagccgatc ataggtgggc gtgatcgcgc cgaacatcgc
     4981 gcggatcttt tcgggcgact gatcatcggc ggcgggggcc gggggggggt cgtcaagggg
     5041 agcggggatc gttgtgaacc gtgggttcat gagcaactcg cggcgcggtc gggtggttcg
     5101 gtcgcttgtt gagcacactt gataaaacca cgcgggctgg gtcatcgtaa cacgcatggg
     5161 tgggcacgaa caaccgctgc cgcgccattt ccgccgaagg tcgagcgccg tggcgcgctg
     5221 ggcgctgttt ttcgcgctcg tcgcctgtgt gctctccgcg agcaatctgc tgctgatctg
     5281 gcgcgacggg gaactcgtac gcaacgcgcg gctgctgacc gaggagatcg ggcgccagtt
     5341 ggacaagcgg cgcgaagcga ggcagagcga gacgcccgcc gcgccgaccg gcgagcggcc
     5401 ggcggacgag cggtcggccg atacgggcgg cgccgatcag ccggcggcgg gcccgctcaa
     5461 cgaagcgctg gacaacctga gctggcggct ggaacggatc ggcgcgatga tccgcgacaa
     5521 ggatgcgcgg gcgcgattgc accttgatgt cctgcgcgac gatctggaat catggcggga
     5581 gaccacgacg gcccaagggg gggaatggat cgaccgggcg ctgacggcgg tccaatcggc
     5641 gcgccgtgat ttcgagcaac aggcgcccca ggcggcggac catctgctga cgctcgccgg
     5701 ccagatacgc gaacggatgc ccgcggtggg ccgtttgaac ccgaccaggg aaccgatggc
     5761 gccggcaagc gaagcgggcg ccgaaccctg aaacgacgcc ccaacggtcc cgttgcccgc
     5821 ccgacgccga ttcccgacga aggttgcgcc gccatggccg aaacaagttt ctaccgcatc
     5881 ggtgtgctgt ccgatacgca cgggtatctg cacccggcgc tgttcgatct gtttgacggg
     5941 gttcagttga tcctgcacgc gggggatatc ggccgggacg acgtgctcgt cgagctgcgg
     6001 acgatcgcgc cggtgcaggc ggtggcgggc aatgtcgacc tgcccgatcc gcgcgcgcgg
     6061 ccgttgatcc ggcaactgga gacgccggcg gggcggatcg cgatgacgca cgggcatctg
     6121 gcccatgcct cggcgcacga tcacgccagc ctgctggggc agttcgccgg gttccagccc
     6181 ggcatcatta tcttcggcca ctcgcatctg ccgctgattg aggaactgga cggcgtcaag
     6241 ctgttcaatc cgggggcggc gggccgcgcg ggcctgaccc agcacggggc gacggtcggg
     6301 atgatctcag tcgaggaagg cagcgggcaa gtcagactgg aacacctgaa gctgagcgcc
     6361 tgagccgccg agcgggacgc gggcgacggc ggcggcgggt tggccgggat tgcaaagcgc
     6421 gcgcccagtt gctacaacac agcggatcag gccacgccgc acgggcggcg ggggccgctc
     6481 ggactgccac ggctccgccc gggcggagca agacaggttg ccccatgtgc cgaccgcgct
     6541 ttgcgcgccg cctgacggcg ctgctgctga tgaccggcct gctggccccg cgggggtcgg
     6601 cccaggactt cgacccgccg ctggggcatg ccgccgagat gatcgacggg gggacggtcg
     6661 cgcgcgttga gatcccgacg caggcggcgg aactgcagac ttacccaatg ccgaccgaat
     6721 acccgcgcca gttgctgcag gaggtgccgg ggcggccgtt ccggccggtg gacccgccgc
     6781 cgcaggcgtt cgcgtggtgg aaggtgccgt tctatgtggc gctggggctg ccgcgcgatc
     6841 tggtggacgg attcgtcggc gggctcgcca aagtgccgat catcagcccg gtgttcgtct
     6901 tcccggcgta tgagctggtg ccgacgcaga ttgtttttcg cgacccgcgc gactggcacc
     6961 gctggccggc gggtcccaac cggcacgatc acggatacta caagggcaat tcgtggggct
     7021 ggtttccttc ggcccacatg tggaaattcc actacacgag cgagcggaag ctggagcgca
     7081 accgcgctta caacgagcgg ctgcaggccg agctgcggga ccgcaaccgt gagatcgaaa
     7141 cgcacaacca actgatcgag aaccgcaagc gcgaggcgcg ccgggaagcg ctgcgtgcga
     7201 tcgaagcggg caacgggcgc gaagccgccc tgcggatgat cccctactat caggcctatc
     7261 cgctggatga gggggccttc gcgctgttcg cgacggcgct ggcgatgtac gcgccggagg
     7321 gacccgagtg ggtgaaaccg ctgctgtggt atgaactgaa tgcggcgcag ccgctgccgc
     7381 tgacgcaggc cgagacgcaa ctggcgctgg cgcgcgagca gcatcccgag cgcgcgtcgc
     7441 tggcggaggc gctgatctac gtgaggttgc tgctgggtga accgcgcgac gcgctggtcg
     7501 tggcggcgga gcacctgacg cgcgaaccgg acgacgcgcg ccggcggcgg ctggtgctgg
     7561 aaacggcgct gagcgcggac gacctggcca cggcgcgcca ggtgtatcag gcattggatt
     7621 cgtcgacgct ggagacgacc gacgcggcgc tgatccgcgc ccgcatggat ctgctggccg
     7681 gagactaccg cggggcccag caactgctgc ggatcatgca ccaggagcat cccgaggatg
     7741 cgtatgtgag ctactacctg gggattgcgg agctgcagct ggcgctgtat gagccggcct
     7801 accccgaggg gt
//
LOCUS       DAOPCO010000189         7764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8538_length_7764_cov_5.390064, whole genome shotgun sequence.
ACCESSION   DAOPCO010000189 DAOPCO010000000
VERSION     DAOPCO010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8538_length_7764_cov_5.390064"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLS90_12230"
     CDS             <1..713
                     /locus_tag="PLS90_12230"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPK03214.1"
                     /translation="ARRLRLVELAYPWRARWLLDDFADALAAKLDELGIESAHLLAES
                     FGSIVGWQFALTRPRRVRSLLLAGGFCRPPWSSFLPAALATMAAIPPRWVDSVIGAYI
                     RLHAGRRDYDADAGFPPFSASRGREGWRACRNRLRVIRATDLRRRLGEIRCPVAYVGG
                     GRDLVVPVRREIRCLRESLHPDCRFTQWIVEGAPHPILPARPQSVANWIADRVEEAER
                     MRSGAAEERELGVSKKDK"
     gene            complement(876..1055)
                     /locus_tag="PLS90_12235"
     CDS             complement(876..1055)
                     /locus_tag="PLS90_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03215.1"
                     /translation="MNRIALRYLPERNLYHLVWNEKEGTVTRASLESFGSRQITQLAD
                     RSQDRPEQWCVARLH"
     gene            complement(1539..1614)
                     /locus_tag="PLS90_12240"
     tRNA            complement(1539..1614)
                     /locus_tag="PLS90_12240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1579..1581),aa:Thr,seq:cgt)
     gene            complement(1725..2618)
                     /locus_tag="PLS90_12245"
     CDS             complement(1725..2618)
                     /locus_tag="PLS90_12245"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HPK03216.1"
                     /translation="MRTFRPRSPLTTAPRRPILPLLALLACLAAAGCARGGASLAPRP
                     FDPAYQSWNETLVKYATPDGVDYAALAADPDPLWRTIAELRDITPHQFGTWSRAARLA
                     CLINAHNAYALKRIVDHWPVKSLRRTRLALPARDARDAVLLGRRWSLRELEERIMSDE
                     FMESRAIFLLNWGERGCAPLPIFPVTELNLRDLLDRQTRAFINDPRYVRYAPLDTEIY
                     VSPLLDWYGPQLTRDFTTLWVLIGQYLPEAEAEAFSRRPPRIHFLDFDRGLNRVEQAI
                     PAEARGAIEAGVLPEPGAPAP"
     gene            complement(2634..3128)
                     /locus_tag="PLS90_12250"
     CDS             complement(2634..3128)
                     /locus_tag="PLS90_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPK03217.1"
                     /translation="MSPALAPFARLRSRLNALQAWGARRATLYYLGPPVVWCVVVLAL
                     SLAPPSPRIRRIDVPNLDKLGHALMYTLLGALIVRAIVRRRAPRAGALAVAVVLAGLY
                     GLALEGLQGLTSWRTFDPFDALANLLGAGLGVALWSLVSKGLAKAPAAGEDVNPTAPR
                     RPPR"
     gene            3308..3775
                     /gene="hisI"
                     /locus_tag="PLS90_12255"
     CDS             3308..3775
                     /gene="hisI"
                     /locus_tag="PLS90_12255"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPK03218.1"
                     /translation="MSSQVIFAERGDKRQVEQGDRLAPKFNADGLLPVVTTDYATGEV
                     LMQAWMNAEALARTIELGEAVYWSRSRQELWHKGATSGLVQKVRELRVDCDQDAVWLR
                     VDVVGEGCCHVGYRSCFYRRVPLGEPAAAGAPVRLELTEDEKAFDPEKVYGKK"
     gene            3832..4845
                     /gene="aroF"
                     /locus_tag="PLS90_12260"
     CDS             3832..4845
                     /gene="aroF"
                     /locus_tag="PLS90_12260"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPK03219.1"
                     /translation="MIIVMKPHSPARQIDDVIHRIEELGYRAHPIYGVERTVIGCVGR
                     EDKSPLSQLESMPGVEAAIPILKPYKLASREFRPEPSVLDVAGVAVGGERLAIVAGPC
                     SVESRAQTLEIAHAVREAGAQFLRGGAFKPRTSPYAFQGLGAEGLEILAEAREQTGLK
                     LVTEVVGVEDLPAMVDVVDVFQIGARNCQNYPLLNAVGQTRKPVVLKRGMATLIREWL
                     MAAEYILAHGNYNVILCERGIRTFETATRFTLDLNAVPVLKETTHLPVLVDPSHGTGQ
                     WQFVEPMARAAIAAGADGLMIEVHQDPPNAASDGAQSLKPEKFARLIAETRKIALAMG
                     RQI"
     gene            5295..6731
                     /locus_tag="PLS90_12265"
     CDS             5295..6731
                     /locus_tag="PLS90_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK03220.1"
                     /translation="MALPVNTNVSSINAQRNLQRSTLALGKSLERLSSGLRINRAGDD
                     AAGLAISEGLRSQIRGLTQAVRNANDGLSLIGTAESAIGTYTDILQRVRELAIQSAND
                     TNSHNNRQALNEESQQLLEELQRIATTVEFNGSRLLDGTFQAKQLQVGAQANQTISID
                     TGNLLTHNLGKVARATGSAVPAGAALGTGELLINGVDIESSQPDGVSTVGSSGSAIAI
                     ANAINAKTNLHNVKAEALAAVFSGGQAIAAGSVNGTTEQLTINGIKIFDTATTILADD
                     SSGALRDAINAKSNQTGVVASLDTSNQLVLTAADGRNIHVVSTGSVGDHLGLTAGAAD
                     HTSTTGGTVRITSVNNITVSGSAPNKAGLTATTYTELASTSVNYIDLSSVDGANLAID
                     IVDKALEEINSIRAGLGAITNRLELTVQNLQSISENLSASESRIRDADFAVETATLTR
                     NQILQQAGIAILSQSNTTTQSALQLLQR"
     gene            7007..7261
                     /locus_tag="PLS90_12270"
     CDS             7007..7261
                     /locus_tag="PLS90_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK03221.1"
                     /translation="MPKEAIAMPDSRRAGRPAAEAGREYWRVDRVARYLDISRKRVYQ
                     LVQEGHLRKIRIGARQMRIDRQSLEDYIARKAADAAAEAP"
     gene            complement(7293..>7764)
                     /locus_tag="PLS90_12275"
     CDS             complement(7293..>7764)
                     /locus_tag="PLS90_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK03222.1"
                     /translation="DFLGARWSLLTADGWRHIILPALTLASYHTAMLIRLVRSEMAET
                     LRQPFIRVCRARGLSERSVIARHALRNTLIPTVTVIGLQFGGLLAFSVVTEHIFQWPG
                     LGKLLIDSVQVDRPVVVTYLILTGVLFLAINFVVDLTYALIDPRIRWSAARSEP"
BASE COUNT         1342 a         2540 c         2731 g         1151 t
ORIGIN      
        1 tggcacgccg cctgcggctg gtcgagctgg cctacccctg gcgcgcgcgc tggctgctgg
       61 atgatttcgc cgacgcgctg gcggcgaagc tggacgagct ggggatcgaa tccgcccatc
      121 tgctcgccga gtcgttcggt tcgatcgtcg gctggcagtt cgcgctgacc cggccgcggc
      181 gtgtccgctc gctgctgctg gccggcggat tctgccggcc gccgtggagt tcgttcctgc
      241 cggccgcgct ggcgacgatg gccgcgatcc ccccgcgctg ggtcgactcc gtcatcgggg
      301 cctacatccg gctgcatgcg gggcggcgcg actatgatgc cgacgcgggg tttccgccgt
      361 tcagcgccag ccgcggccgc gaggggtggc gcgcctgccg caaccggctg cgcgtgatcc
      421 gcgccaccga cctgcggcgg cgcctgggcg agatccggtg cccggtggca tatgtcggcg
      481 gcgggcgcga tctggtcgtc cccgtgcgcc gcgagatccg ctgcctgcgc gagtcgctcc
      541 atcccgactg ccggttcacg cagtggatcg tggagggggc gccccatccg atcctccccg
      601 cgcggcccca gagcgtggcg aactggatcg ccgaccgcgt cgaggaagcg gagcggatgc
      661 gctccggcgc ggcggaggag cgggagttgg gggtatccaa aaaggacaag taaaaacccc
      721 cggagcgagc gctcgcgggg gcgtgccggt cagaaaatga cgtgggcccg gcggctgaaa
      781 ccagcaaggg aagagacgtt tcccaccggt tgccgcccag gcccgtgcgg gcggctgcaa
      841 tcaagagtca tgcgctgatt cgtttcagcg gccgatcagt gcaggcgcgc gacgcaccac
      901 tgctcgggcc ggtcctggct gcggtcggcc agttgggtga tctggcggga gccgaagctc
      961 tccaggctcg cacgggtcac cgtgccttcc ttttcgttcc acacgagatg atagaggtta
     1021 cgctcgggaa ggtaacggag tgcgatccga ttcatgaggt ccttctcctt gcaatcgctc
     1081 cgccggccga acggccagcc tggtgtgtct cgacaagtta attttacagg aacccccggg
     1141 ccgaaccaac caaaatccgg ctcggccggc gcaagccggg gcgttgtctc tccctagtgc
     1201 ccctggtttt ttgttgggcg ccgatggcgc ctgatccttg taacggccaa ccccaccggt
     1261 ttaagaaccg gagttcggtt tctgatacaa ccttccgcga ggggaaaccg ggaggatcac
     1321 gggaggcggg ggagggcaag cccctgcaaa ctcagtatga ttgatgcttg cggttcggaa
     1381 gcagagcggc atcgatgatc gccgcgcggt ctcagccccc gcattccggg gaggaagcgc
     1441 cggcgaatcg atctcgattt tgatcccgat accgatcccg aatccggcgg gtcgtctccg
     1501 ataggcctta tttttggaga gttcttgagg aattgagctg gagctgatgg tcggactcga
     1561 accgacgacc tgcgcattac gagtgcgctg ctctaccagc tgagctacat cagcccacgc
     1621 gaacccccta tacttatcaa tccggccggg ggcttgtcaa gctgacggcc gcagggccac
     1681 gggcggcaat agcgatccga aatccgccgc gaacgcgatc cgcatcaggg ggcgggcgcg
     1741 ccgggctcgg ggaggacgcc ggcttcgatc gcgccgcgcg cttcggccgg gatcgcctgt
     1801 tccacccggt tcaggccgcg gtcgaaatcg aggaagtgga tgcgcggcgg ccgccggctg
     1861 aacgcctcgg cctcggcctc gggcagatac tggccgatca ggacccacag cgtcgtgaaa
     1921 tcgcgcgtga gctgggggcc ataccagtcc agcagcggcg agacgtagat ctccgtgtcg
     1981 agcggggcat agcgcacgta gcgcgggtcg ttgatgaacg cccgcgtctg ccggtcgagc
     2041 aggtcgcgca gattcagttc ggtgacgggg aagatcggca gcggggcgca tccccgctcg
     2101 ccccagttga gcaggaagat ggcgcgcgac tccatgaact cgtcgctcat aatgcgctcc
     2161 tccagctcgc gcagcgacca ccggcggccc agcaggacgg catcgcgcgc gtcgcgcgcg
     2221 gggagcgcca ggcgcgtgcg ccggagcgac ttgaccggcc agtggtcgac gatccgcttg
     2281 agcgcatagg cgttgtgggc attgatcagg cacgccaggc gcgcggcgcg cgaccaggtc
     2341 ccgaactggt gcggggtgat gtcgcgcagc tcggcgatcg tccgccagag cgggtcggga
     2401 tcggcggcga gcgcggcata gtcgacgccg tcgggggtcg cgtatttgac cagcgtctca
     2461 ttccagctct gataggcggg gtcgaacggc cggggggcaa gggaggcgcc gccgcgcgcg
     2521 cagccggccg ccgcaaggca ggccagcagt gccagcaggg ggaggatcgg gcggcgcggg
     2581 gccgtggtga gtggggagcg ggggcggaac gtgcgcatca ggcgcctcat caatcatctg
     2641 ggggggcggc ggggggccgt cgggttgaca tcttcccccg cggccggcgc tttggccagt
     2701 ccttttgaaa cgagcgacca gagggcgacg cccaggcccg cgccgagcag gttggccagc
     2761 gcatcgaagg ggtcgaaggt gcgccacgag gtcaatccct ggaggccttc cagcgccagg
     2821 ccatagaggc cggccagcac gacggcgacc gccagcgccc cggcgcgggg ggcgcgccgg
     2881 cgcacgatcg cgcggacgat cagcgccccc agcagcgtat acatcagcgc atggccgagt
     2941 ttgtccaggt tgggcacgtc gatgcgcctg atccgcggcg agggcggggc gaggctgagc
     3001 gccaggacga cgacgcacca caccaccggc gggccgagat aatatagcgt cgcgcggcgt
     3061 gcgccccatg cctgcagggc attcagacgc gagcggaggc gggcgaaggg agcgagagcc
     3121 ggtgacatgg aggagcacag ccgcagcggg agttggaccg gccgacagta tgtcagagcg
     3181 acccccggaa atcaccgctc cattccaata cgcccccaga attgacttgc ccccgaaagg
     3241 acggcagaat gtgaggattc cggtggtccg accaggtcgc cggcgacccg ccagggagga
     3301 ttcagggatg agcagccaag taattttcgc cgagcgcggc gacaagcggc aggtcgagca
     3361 gggcgaccgg ctggcgccga aattcaacgc cgacgggctg ttgccggtgg tgacgaccga
     3421 ttacgcgacc ggggaagtgc tgatgcaggc ttggatgaac gccgaggcgc tcgcccgcac
     3481 gatcgagctg ggcgaggcgg tctactggag ccgcagccgg caggagctgt ggcacaaggg
     3541 cgccaccagc ggcctggtcc agaaggtccg cgagctgcgg gtcgattgcg accaggacgc
     3601 cgtctggctg cgcgtcgacg tcgtcggcga gggctgctgc cacgtcggct accgttcgtg
     3661 cttctatcgg cgtgtgccgc tgggcgaacc ggccgccgcc ggcgcgcccg tgcggctgga
     3721 gctgaccgag gatgagaagg ccttcgatcc ggagaaggtg tacgggaaga agtaggcggg
     3781 ggtgaggacg aaggacgagg acgatttcag gatagggatg agggggcgca ggtgatcatt
     3841 gtcatgaaac cccacagccc ggcgcggcag atcgacgatg tgatccaccg catcgaagag
     3901 ctgggctacc gcgcgcaccc gatctacggc gtcgagcgca cggtgatcgg ctgcgtcggc
     3961 cgcgaggaca agagcccgct gagccagctc gaaagcatgc cgggggtcga ggcggcgatt
     4021 ccgattctca agccgtacaa gctggccagc cgcgagttcc gccccgagcc gtcggtgctg
     4081 gatgtcgcgg gcgtggcggt cggcggcgag cggctggcga tcgtcgcggg gccgtgctcg
     4141 gtcgagtcgc gcgcccagac gctcgagatc gcgcatgccg tccgcgaggc gggcgcccag
     4201 ttcctgcgtg gcggcgcgtt caagccgcgc acgtcgccct acgcctttca gggcctgggc
     4261 gccgagggcc tggagatcct cgccgaagcg cgcgaacaga cgggcctgaa gctcgtcacc
     4321 gaagtcgtcg gggtcgagga cctgccggcg atggtcgatg tcgtcgacgt cttccagatc
     4381 ggcgcgcgca actgccagaa ctatccgctg ctcaatgccg tcggccagac gcggaagccc
     4441 gtcgtcctca agcgcggcat ggcgacgctg atccgcgaat ggctgatggc cgcggaatac
     4501 atcctcgccc acggcaacta caacgtgatt ctatgcgaac gggggatccg gacgttcgag
     4561 accgcgacgc gtttcacgct cgacctgaat gcggtccccg tgctgaagga gacgacgcac
     4621 ctgccggtgc tggtcgaccc gagccacggg acgggccaat ggcagtttgt cgagccgatg
     4681 gcgcgggcgg cgatcgccgc gggggccgac ggcctgatga tcgaggtgca ccaggacccg
     4741 cccaatgcgg ccagcgacgg ggcgcagtcg ctcaagccgg agaaattcgc ccggctgatc
     4801 gccgagacgc gcaagatcgc gctggcgatg ggccgacaga tctgattcga ggcctccgcg
     4861 gcggatagat tcgccaaata gctgacgcct ggcggcagtt tctgctggcc gagcgtcaaa
     4921 gaaccgggat ctttttgccg atcagttttc gccgagcggc tcccggcggc tccatcgtgt
     4981 gcggttctgt gccattttcg agcaacgcgc ctgatttttc ctaacccctg ttttttcaag
     5041 tttacgtaga tccgatcaga tgccttcgac ttgatttgca gcggtcccgg cgcgcggttt
     5101 gccgaggcgg cgggcggcgc ttggcttggc gtttgctccc ctgatccgcc cgaatcggat
     5161 tgcaggcccg cctgcccccg ccgattcgga caatgtcatc cccccggaac gccgagcggc
     5221 ggccgggcag ccggctggca gcgacacgga cgacgcggcc ctggccagga taaccacagg
     5281 agagatgacg gatcatggct cttccggtca acacgaacgt ttcgagcatc aatgcccagc
     5341 gcaacctgca gcgatcgacg ctggcgctcg gcaaatccct cgagcggctg tcgtcgggtt
     5401 tgcgcatcaa ccgcgccggg gatgacgccg ccggcctggc gatttccgaa ggcctgcgct
     5461 cgcagatccg cggcctgacg caggccgtgc gcaacgccaa cgacggattg agcctgatcg
     5521 gcacggccga gagcgccatc ggcacctata ccgatattct ccagcgcgtg cgcgagctgg
     5581 cgatccagtc ggccaacgac accaactcgc acaacaaccg ccaggcgctc aacgaggaaa
     5641 gccagcaact gctggaagag ctgcagcgga tcgcgacgac ggtcgaattc aacggctcgc
     5701 gcctgctgga cggcacgttc caggccaaac agcttcaggt gggcgcccag gccaaccaga
     5761 cgatctcgat cgatacgggc aacctgttga cccacaacct gggcaaggtc gcgcgcgcca
     5821 ccgggtcggc ggttccggcc ggcgccgccc tgggaaccgg cgagctgctg atcaacggcg
     5881 tcgacatcga gtcgtcgcag cccgacggcg tttccacggt cggctcgtcg ggatcggcca
     5941 tcgccatcgc caacgcgatc aacgccaaga cgaacctgca caatgtcaaa gcggaggcgc
     6001 tcgcagcggt gttcagcggc ggccaggcga tcgccgccgg ttcggtcaac ggcacgacgg
     6061 aacagctgac gatcaacggg atcaagattt tcgacacggc caccacgatc ctggccgacg
     6121 atagctcggg cgcgctgcgc gacgcgatca acgccaagtc gaaccagacc ggcgtcgtcg
     6181 cctcgctcga tacgagcaac caactggtgc tgaccgccgc cgacgggcgc aacatccatg
     6241 tcgtttcgac cggcagcgtc ggcgaccacc tgggtctgac cgccggcgcc gccgaccaca
     6301 ccagcacgac cggcggcacc gtgcgcatca cctcggtcaa caacatcacc gtcagcggtt
     6361 cggcgcccaa caaggccggc ctgaccgcca cgacctatac ggaattggcc agcacgtcgg
     6421 tcaattacat cgatctttcc tcggtggacg gggccaacct ggccatcgac atcgtcgaca
     6481 aggcgctgga ggagatcaac tcgatccgcg ccggactggg cgccatcacc aaccgcctgg
     6541 aactgacggt ccagaacttg cagtcgattt cggagaatct ctcggcgtcg gagtcgcgca
     6601 tccgcgacgc cgacttcgcc gtcgagaccg ccaccctgac gcgcaaccag atcctgcagc
     6661 aggcaggcat cgccatcctg tcgcagtcca acaccacgac gcaatcggcg ctgcagctcc
     6721 tgcagcgtta agcggcgcca acgttttggc acgggaagac cgctcgtttc ccccgcgagc
     6781 ggttcacagc gaaaacagcc ggaaccaccc ccacggttcc ggctgtttcg ctttgacttg
     6841 ccgcccgctc ggccggctgt ggccggggag tcgccaagcc tgcgtcaagc cgtgcgcaag
     6901 gccgcggcaa gggatcgatg ggccggcagg gcgctatcct cgccgcatca ttcagcggcg
     6961 ccgggccccc ggggggcgcc cgggcgtcgc ggaaaggatg cgagccatgc caaaggaagc
     7021 catcgcgatg ccggacagcc ggcgcgccgg ccggcccgcc gcggaggccg gccgcgagta
     7081 ctggcgcgtc gaccgcgtcg cgcgctatct cgacatcagc cgcaagcgtg tataccagct
     7141 ggttcaggaa gggcatctgc gcaagatccg gatcggggcg cgccagatgc ggatcgaccg
     7201 ccagtcgctg gaggactaca tcgcgcgcaa ggcggccgac gccgcggcgg aggcgccgtg
     7261 acctccgctg ctccccgcgc gcggcgcgcc ggtcagggtt cgctccgggc ggccgaccag
     7321 cggatacggg gatcgatcag ggcataggtc agatccacca cgaagttgat ggccaggaag
     7381 aggacgcccg tcagaatcag ataggtcacg acgaccggcc gatcgacctg caccgagtcg
     7441 atcagcagct tgcccaggcc gggccactga aagatgtgct cggtgacgac ggaaaaggcg
     7501 agcaggccgc cgaactgcag gccgatgacg gtgaccgtcg ggatgagcgt gttgcgcagg
     7561 gcgtggcggg cgatcacgct gcgctcgctc aacccgcgcg cgcggcagac gcggatgaac
     7621 ggctggcgca gcgtctcggc catttccgag cggacgagcc ggatcagcat cgcggtgtga
     7681 tacgaggcca gcgtcagtgc cggcaggatg atgtggcgcc agccgtcggc cgtcagcagg
     7741 ctccagcgcg cgccgaggaa gtcg
//
LOCUS       DAOPCO010000190         7642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8698_length_7642_cov_5.021616, whole genome shotgun sequence.
ACCESSION   DAOPCO010000190 DAOPCO010000000
VERSION     DAOPCO010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8698_length_7642_cov_5.021616"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1362)
                     /gene="tssG"
                     /locus_tag="PLS90_12280"
     CDS             complement(277..1362)
                     /gene="tssG"
                     /locus_tag="PLS90_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HPK03223.1"
                     /translation="MASTTGTTDHPVSGAPAAPPAQRSARPSLLDDLLRHPRRYTFFQ
                     ALRILERYCGGEPIGRAGLPRREAIRLRAHAALSFPAWDLEEAEELPNPWRQPETRYR
                     LTVTFMGVYGPASPLPANYTELLIRPEDDETDEDRERVRDFIDLFHHRKLSFVYRCMA
                     KYRYHLLFEPGARDHFSRYMLSLFGRGTRGLEGWNRPVHPERLIRYAGLLMQQPRCAA
                     GLESLLRDFLRLKISVQQCVGRWLRVEDRNQLGGAFCALGQDLVVGSSIYDRAGKIRI
                     SVGPVGFAKFKDFLPSGKLTGQLRELVRMYLVDELEFDLEVWLKGDEIPPLQMGGGPD
                     AAMLGWTSWAVTGPSPDRAVVFSCQAP"
     gene            complement(1326..3149)
                     /gene="tssF"
                     /locus_tag="PLS90_12285"
     CDS             complement(1326..3149)
                     /gene="tssF"
                     /locus_tag="PLS90_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HPK03224.1"
                     /translation="MFNRYYQDELTFLREMGKEFADAHPKTANLLSDRSADPDVERLL
                     EGFAFLAGQIRQKIDDELPEVTHSLLGILCPHYLRPVPSMTIVQFQPGPEVDAVMRIE
                     PGAELDAVMVHETACRFRTTYPIDLTPLEVSEAVLEAPLSAGHSLRLRFQALNNATIS
                     KLGLTRLRIYIHGDPHISYEAMLRLRRHVARIQVSAVIGGRAVHGFTLPPSAVHQVGF
                     GIDEGILPYPEQSFLGYRLLQEYFALPEKFLFFDLIGLGPLAEIDADDAFEIRFEFDR
                     PGENNLYLTAGNFLLNCTPAINLFKHDADPIHIDQTRTEYRIRPSGGNLEHYEIYSVD
                     ETIGYVTGSAERVIYEPFYSFTHFHHHGTPGRGYYQTRIHNMPRPREDDEEIEVYQMI
                     TQRGATIGHGTDTYISFVSLEQAAAVPLAETISIKLTCSNRHLPEQLNVADIRVPTET
                     SPEFASFRNITKPTSSVHPPLDGGLHWRLISHLSLNFISLIDVDALRGILELYNLQAH
                     YDQQAARENEQRLAGIKSISMKPAEWVYHGAPIRGRHIFMELSEDHFTGEGDMHLFAE
                     MLSHFFALYASVNSFTQLTVRGAIRGEVYTWPRRLGQQIIL"
     gene            complement(3263..3679)
                     /gene="tssE"
                     /locus_tag="PLS90_12290"
     CDS             complement(3263..3679)
                     /gene="tssE"
                     /locus_tag="PLS90_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HPK03225.1"
                     /translation="MANERTLIERLMKPEVRSARSARERKEQLTASVINHLSRLLNSR
                     QGCSLTTPDYGMPDFNESLGTKNEMQAAFENAIRTTILTYEPRLRRVTVRLEEEDGPR
                     LNPRFSITAELVSNDDFPKQVSFSTIMDPAGKLNIW"
     gene            complement(3877..4371)
                     /locus_tag="PLS90_12295"
     CDS             complement(3877..4371)
                     /locus_tag="PLS90_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HPK03226.1"
                     /translation="MPMPFHMWCTGMNQGAIEGGDGVSDIDQREGSVLCQALDHNVAI
                     PRDPQTGLPTGKRVHHPLKITKVYDRSSPKLYQALCTGERFSDVTFKFFRISPNGTEE
                     HYFTITLRDAIIVDIRNWIPECLDPNLEHFRHMEDVSFTYKNIIWRWEENGVEAEDNW
                     ATPK"
     gene            complement(4559..6058)
                     /gene="tssC"
                     /locus_tag="PLS90_12300"
     CDS             complement(4559..6058)
                     /gene="tssC"
                     /locus_tag="PLS90_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HPK03227.1"
                     /translation="MADSPQTEQQSAQATESVAEGTPSLISEILEQTNIAPGDGEAFQ
                     LASTGMRAFMRELLKSERSGEKVDKRVVDEMIALIDQRLSKQVDAVLHHPTFQQLESA
                     WRGLRLVVERTNFRENIKIEVLNVSKEDLKNDFDLELDITKTGLYRRVYTAEYGQFGG
                     EPYGALIANYDFGPGPQDMALLQKCAAVATMAHAPFIAAASPTFFGTKDLTSLPNMKD
                     IKSILEGPQYTKWQSFRESEDARYVGLTLPRFLLRLPYGKDTNPIKAFDYEENVHEKH
                     DDYLWGNTAFAFATRLTDSFAKYRWCPNIIGPQGGGAMEDLPLHQYEAMGEIQTKIPT
                     EVLISERREFELSEEGFIPLTMRKGSDNACFFSANSCQKPKNFGQSAEGKEAETNYRL
                     GTQLPYMFVVSRLAHYVKVIQRELIGSTMERVDLENNLNQWIKQYVADMDNASAGVRA
                     RRPLRQAQIMVENVEGNPGWYRVDMKVRPHFKYMGAFFTLSLVGKLDKK"
     gene            complement(6107..6595)
                     /gene="tssB"
                     /locus_tag="PLS90_12305"
     CDS             complement(6107..6595)
                     /gene="tssB"
                     /locus_tag="PLS90_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HPK03228.1"
                     /translation="MAKEGSVAPQERVNIVYKPATGGAQEEKELPLKLLMLGDYTLRE
                     DDRALEDRDPIKIDKDNFDEVMRNQNLSLNLEVDNRLVDDSDEQLAVKLKISSMKDFE
                     PESIARQMPETNKLLQLRQALNALKAPLANKREFRKKIEAILADETARQKLLDELGIG
                     DE"
     gene            complement(6965..>7642)
                     /locus_tag="PLS90_12310"
     CDS             complement(6965..>7642)
                     /locus_tag="PLS90_12310"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPK03229.1"
                     /translation="LVYRSFGFQTAVEEADRVLDCAHVEATGARGGVGLVKLMGRHCG
                     FIAAHATLANGDVNYCLIPEIPFRLEGEKGLLAHLRDRLRRRRHAVIVVAEGAGTELI
                     GLSDRKDASGNRLLNDIGLYLKDEISRQFRAWGDEVHLKYFDPSYIIRSVPANSNDSI
                     FCADLARFAVHAAMAGKTDLLIGQWHGIFTHIPLTAIIGKRKQIDPEGNLWRSVLATT
                     GQPARWS"
BASE COUNT         1351 a         2461 c         2401 g         1429 t
ORIGIN      
        1 tcccccggca accccaaacg cccgcatccc ggcagggatg ccagcaggta gccggcggta
       61 acctacgttt ccgccctcgt ccccggacat cctgagtccg cgaagcggac gaccggaata
      121 aagcgcaggg tgacggccgc gggccggaac cctgcgtcgc tccccaaagc cccaagcccc
      181 tggaaggggc ggccggagcc ggtctgaatt tgcggcatgt cgatcatatc ccgcgtcccg
      241 cgcgaggacg cggacacgag acggccggcg aattcctcag ggcgcctgac aactgaaaac
      301 gacggcgcgg tcggggctcg gccccgtcac cgcccagctc gtccagccga gcatcgccgc
      361 gtcggggccg ccgcccatct gcagcggcgg aatctcgtcc cccttgagcc agacctccag
      421 atcgaattcc agctcatcga ccagatacat ccgcaccagc tcgcgcaact gcccggtcag
      481 cttgcccgag ggaaggaaat ccttgaactt ggcgaagccg accggaccga ccgagatgcg
      541 gatcttgccg gcgcgatcgt agatcgagga gccgacgacc agatcctgcc cgagcgcgca
      601 gaacgcgccg cccagctgat tgcggtcctc gacgcgcagc cagcgaccga cgcactgctg
      661 gacgctgatc ttcagccgca ggaaatcgcg cagcaggctt tccagtccgg ccgcgcagcg
      721 cggctgttgc atcagcagcc cggcgtatcg gatcagccgc tcgggatgga ccgggcgatt
      781 ccacccctcc aggccgcgcg tgccgcgacc gaacagcgaa agcatgtagc gcgaaaaatg
      841 gtcgcgcgcc cccggctcga acagcaggtg atagcgatac ttggccatgc agcggtagac
      901 gaacgacagc ttgcggtgat gaaacagatc gatgaaatcg cggacgcgct cgcgatcctc
      961 gtccgtttca tcgtcctcgg ggcggatcag cagttcggtg tagttggcgg gcaatggcga
     1021 cgccgggccg tagaccccca tgaacgtgac cgtcaggcgg tagcgtgttt cgggctggcg
     1081 ccagggattg gggagttcct cggcctcctc gaggtcccac gcgggaaaac tgagcgcggc
     1141 gtgggcgcgc aggcgaatcg cctcgcgccg cggcagcccg gcgcggccga tcggctcgcc
     1201 gccgcagtat cgctccagga tccgcagcgc ctggaagaac gtgtatcggc ggggatgacg
     1261 cagcagatcg tcgagcagcg acggacgcgc actgcgttgc gccggcggcg ccgccggcgc
     1321 tccgcttaca ggatgatctg ttgtcccagt cgtcgaggcc atgtgtatac ctcaccgcgg
     1381 atggccccgc ggacggtcag ttgagtaaac gaattgacgc tcgcatacag cgcgaagaag
     1441 tggctgagca tctcggcgaa caggtgcatg tcgccttcgc cggtgaaatg atcctcgctc
     1501 agctccatga agatgtggcg gccgcggatc ggcgcgccgt gatacaccca ttcggccggc
     1561 ttcatcgaga tcgacttgat gccggccagc cgctgctcgt tctcgcgcgc ggcctgctgg
     1621 tcatagtgcg cctgcaggtt atacagctcg aggatgccgc gcagcgcgtc gacgtcgatc
     1681 agcgagatga agttgagcga cagatgcgaa atcaaccgcc agtggaggcc gccgtcgagc
     1741 ggcggatgca ccgaactggt cggcttggtg atgttgcgga agctcgcgaa ttcgggcgag
     1801 gtttccgtcg gcacgcgaat gtcggccacg ttgagctgct cgggcagatg gcggttggag
     1861 caggtcagct tgatcgagat cgtttcggcc agcggcaccg ccgcggcctg ttccagcgag
     1921 acgaacgaga tatacgtatc ggtgccgtgg ccgatcgtcg ccccccgctg cgtgatcatc
     1981 tggtagacct cgatctcctc gtcgtcctcg cgcggccgcg gcatgttgtg gatccgggtc
     2041 tgatagtagc cgcgtccggg cgtcccatga tgatggaagt gcgtgaaact gtagaacggt
     2101 tcgtagatca cgcgctcggc gctgccggtc acatagccga tcgtctcgtc gaccgaatag
     2161 atctcgtagt gttccagatt gcccccgctc ggccggatgc ggtattcggt ccgcgtctga
     2221 tcgatgtgaa tcgggtcggc gtcgtgcttg aacaggttga tcgccggcgt gcagttgagc
     2281 aggaaattgc cggccgtcag gtagaggttg ttctcgccgg ggcggtcgaa ctcgaagcgg
     2341 atttcaaacg cgtcgtcggc gtcaatttca gccagcgggc ccaggccgat cagatcgaaa
     2401 aacaggaact tctcgggcag cgcgaaatac tcctgcagca gtcgatagcc caggaaactc
     2461 tgctcggggt agggcaggat cccctcatcg atgccgaagc cgacctgatg gacggcggag
     2521 ggcggcagcg tgaacccgtg aacggcgcgc ccgccgatca cggcgctgac ctgaatccgg
     2581 gcgacatggc gccgcagccg cagcatcgcc tcgtagctga tgtgcgggtc gccatggatg
     2641 tagatccgca accgggtcag cccgagcttc gaaatcgttg cattattgag cgcctgaaac
     2701 cgcaggcgca ggctgtggcc ggccgacagc ggcgcctcca ggacggcctc gctgacttcc
     2761 agcggcgtca gatcgatggg gtaggttgtg cggaaccggc acgcggtctc gtgcaccatc
     2821 acggcgtcga gctcagcccc gggctcgatt cgcatcaccg cgtccacttc gggtcccggc
     2881 tggaactgga cgatcgtcat cgacgggacc ggccgcaggt aatgcgggca gagaatgccg
     2941 aggagcgaat gcgtgacctc gggcaattcg tcgtcgattt tctggcggat ctggccggcg
     3001 agaaaggcga acccctccag cagccgctcg acatccgggt cggccgaccg gtccgagagc
     3061 aggttggcgg ttttcggatg ggcgtcggca aactccttgc ccatctcgcg cagaaaggtc
     3121 agttcgtcct ggtagtagcg gttaaacacg cgtcgcaaat cctcctggct tcgtcggcgc
     3181 tcggcggcgc gtcggttcgc cccatggcgg aatcagacgg gccgaggcga cgcggcagcg
     3241 cacaccggct ggcggaatcg ggtcaccaga tattgagttt accggccggg tccatgatgg
     3301 tcgagaagga aacctgcttg ggaaaatcgt cgttcgagac cagctcggcg gtaatcgaaa
     3361 accgcggatt cagccgcggg ccgtcctctt cctccagccg caccgtgacg cggcgcagac
     3421 gcggctcgta ggtgagaatc gtcgtgcgga tcgcattctc gaaggcggcc tgcatttcat
     3481 tcttcgtgcc gagcgactcg ttgaagtcgg gcatcccgta gtcgggcgtc gtcaggctgc
     3541 acccctggcg cgagttgagc aggcgcgaca ggtggttgat gaccgaggcg gtcaactgtt
     3601 ccttgcgctc gcgcgccgag cgcgccgagc ggacctcggg tttcatcagg cgttcgatca
     3661 gcgtgcgttc attggccatt gtcagcatcc cctcgcgcgg aaccgccgtc gggagccggc
     3721 acgccgtcgc gcgacgcccc ggccgattaa agaaacgcgc cgacgaaggg tccgcgggga
     3781 ccctccgcca gcgcatttcg tcggccgggc gcgccttcgc gggcgcgcgc ctcaggcggg
     3841 acatcgatcg cacgaagcgc ttccttcgtc aaccgatcac ttcggcgtcg cccagttgtc
     3901 ctcggcttca acgccattct cttcccagcg ccagatgatg ttcttgtagg tgaaagaaac
     3961 atcttccatg tgccggaagt gctcgaggtt cgggtcgagg cactcgggga tccagttgcg
     4021 gatgtcgacg atgatcgcgt cgcgcaacgt gatcgtgaag tagtgttcct cggtcccgtt
     4081 gggcgaaatc cggaagaact tgaacgtcac gtccgagaaa cgctcgcccg tgcaaagcgc
     4141 ctgatacagc ttcggcgagg aacggtcgta gaccttcgtg atcttcagcg gatgatgcac
     4201 gcgcttgccg gtgggcaggc cggtctgggg gtcgcgcgga atggcgacgt tatgatccag
     4261 agcctggcac agcaccgacc cttcgcgctg gtcaatgtcg gagacaccgt caccaccttc
     4321 gatggcgccc tggttcatcc cagtgcacca catgtggaat ggcatcggca tggtgcgatt
     4381 cctttctgtg caatttgttt ggcgtgtggc cgaggtagcc acgcaccgcc agtggttcaa
     4441 agattggttc ttccgtcgga ttgcaactgc tcacctatct ggatagaacg cggcggggat
     4501 ttcgacaacc gaaaaattga gtccggcctc atttctttcg gtcgccgacc gccctacctc
     4561 acttcttgtc cagtttgccg accagcgaca gcgtgaaaaa cgcccccata tacttgaagt
     4621 gcgggcgcac cttcatatcc acccgatacc agcccgggtt cccctcgacg ttctcgacca
     4681 tgatctgcgc ttggcgcagc gggcgccgcg cccgcacccc cgccgacgcg ttgtccatgt
     4741 cggccacata ctgcttgatc cactggttca ggttgttctc caggtcgacg cgctccatcg
     4801 tgctgccgat cagctcgcgc tggatcacct tcacatagtg cgccaggcgc gagacgacga
     4861 acatatacgg caactgcgtc cccagccggt agttcgtctc cgcttccttg ccctcggcgc
     4921 tctggccgaa gttcttcggc ttctggcacg agttggccga gaagaagcag gcgttgtcgc
     4981 ttcccttgcg catcgtcagc gggatgaagc cctcttcgct cagctcgaac tcgcgccgct
     5041 cgctgatcag gacctccgtc gggatcttcg tctggatctc gcccatcgct tcatactggt
     5101 gcagcggcag atcctccatc gcgccgccgc cctgcgggcc gatgatgttc gggcaccagc
     5161 ggtacttcgc gaaactgtcg gtcagccgcg tcgcgaacgc gaacgccgtg ttgccccaca
     5221 ggtagtcgtc gtgtttctcg tggacgtttt cctcgtaatc gaacgccttg atcgggttgg
     5281 tgtccttgcc gtagggcagg cgcagcagga accgcggcag cgtcagcccg acgtagcgcg
     5341 catcctcgct ctcgcggaac gactgccact tcgtatactg cggcccctcc aggatcgact
     5401 tgatgtcctt catgttgggc aggctggtca ggtccttcgt gccgaagaac gtcggcgagg
     5461 cggcggcgat gaacggggca tgggccatcg tcgccacggc cgcgcacttc tgcagcaggg
     5521 ccatgtcctg cgggccgggg ccgaagtcgt agttcgcgat cagcgcgccg tacggctcgc
     5581 cgccgaactg gccgtactcg gccgtgtaca cgcgccggta gagcccggtc ttggtgatgt
     5641 ccagctccag gtcgaaatcg ttcttgaggt cctccttgga gacgttgagc acctcgatct
     5701 tgatgttttc gcggaagttg gtgcgctcga cgaccaaccg cagcccgcgc cacgccgatt
     5761 ccagttgctg gaacgtcggg tgatgcagca cggcatcgac ctgcttgctc agacgctggt
     5821 cgatcagcgc gatcatctca tcgacgacgc gcttgtcgac cttctcgccc gagcgttcgc
     5881 tcttgagcag ctcgcgcatg aacgcgcgca tgcccgtcga ggcgagctgg aacgcctcgc
     5941 cgtcgcccgg cgcgatgttc gtctgctcga gaatctccga aatcagcgat ggggtgcctt
     6001 ccgccacgct ttcggttgcc tgggccgatt gttgttcggt ttgagggctg tcagccatcg
     6061 tcatcaactc ctcggggatg ggatcttacg ccatgcgcga tacgcgctac tcgtcgccga
     6121 tgcctagctc atcgagcagc ttctgacgag ccgtctcatc ggccaggatc gcttcgatct
     6181 tcttccggaa ttcgcgtttg ttggccagtg gggccttcag cgcgttgagc gcctggcgca
     6241 actgcagcag cttgttggtc tcgggcatct gccgcgcgat cgactcgggc tcgaaatcct
     6301 tcatcgagga gatcttcagc ttcaccgcga gctgctcgtc cgagtcatcg accagacggt
     6361 tgtccacttc gaggttgagc gacagattct ggttgcgcat cacctcgtcg aaattgtcct
     6421 tgtcgatctt gatcggatcg cgatcttcca gcgcccgatc atcctcgcgc aacgtgtaat
     6481 cgcccagcat cagcagcttg agcggcagtt ccttttcttc ctgcgcgccg ccggtcgcgg
     6541 gcttgtagac gatattgacc cgctcttgcg gtgcgactga gccttccttc gccatgggcg
     6601 catcacctca ctgaaagaaa tgaagaaaca ttgagagaac cgcatcggtc tgcggaggga
     6661 gccatgttcc gccgtacgag tgtcgctcga taggaagcca tgctacggcg cgacggcgat
     6721 aaaacgcaag cacaatgtcg agaatgaaga agaaaaataa atcccgcggg catcaataac
     6781 ggaaaaacga accctgatat ttgaatattc tcgccgctcc gccccgatcg gggattttcc
     6841 ctgctcacga aaatcacgaa gctcacggat cgcgaaaatc acgaagatca cgaaaaacac
     6901 gcaggacgcc gcgcgaactg gtccccaccg ccggccgccg cgcgatgcgt gccccgtgcc
     6961 ccagtcagct ccagcgcgcc ggctgacccg tcgtcgccag cacgctgcgc cacagattcc
     7021 cctccgggtc gatctgcttg cgcttgccaa tgatcgccgt cagcggaata tgcgtgaaga
     7081 tcccgtgcca ttggccgatc agcagatcgg tcttgcccgc catcgccgcg tggaccgcga
     7141 accgcgccag gtcggcgcag aagatcgagt cgttgctgtt ggccggtacg ctccgaatga
     7201 tgtagctcgg atcgaaatac ttgaggtgga cctcgtcccc ccatgcccgg aactggcggc
     7261 tgatctcatc cttcaggtag aggccgatgt cattgagcaa tcggttgccc gaggcatcct
     7321 tgcggtccga caggccgatc agctcggtgc ccgccccctc ggccacgacg atcaccgcgt
     7381 ggcgccgccg acgcagccgg tcgcgcagat gggccagcag ccccttttcc ccctcgagcc
     7441 ggaacgggat ctccggaatc aggcagtagt tcacgtcgcc gttggccagc gtcgcatgcg
     7501 ccgcgatgaa cccgcaatgg cggcccatca gcttcaccag cccgaccccg ccgcgggcgc
     7561 ccgtcgcctc cacatgcgcg caatccagca cgcggtcggc ctcctcgacg gcggtctgaa
     7621 agccaaaact gcggtagacg ag
//
LOCUS       DAOPCO010000191         7593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8763_length_7593_cov_4.898647, whole genome shotgun sequence.
ACCESSION   DAOPCO010000191 DAOPCO010000000
VERSION     DAOPCO010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7593
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8763_length_7593_cov_4.898647"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..1339)
                     /gene="queA"
                     /locus_tag="PLS90_12315"
     CDS             complement(287..1339)
                     /gene="queA"
                     /locus_tag="PLS90_12315"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008371840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPK03230.1"
                     /translation="MQLADFQYQLPEELIATHPAARRDDSRLLVLARRTGAISHHRFR
                     DLSALLRPGDCLVLNDSRVIPARLRARRAGGGAAELLLLRSLGDGTWEALARPARKLK
                     PGARLDLPGGGEARVLRDGPDGTRVVALHVEGDVEDYLERAGEAPLPPYIVQQRKARG
                     EAPAAGEDTERYQTVYARAQGSVAAPTAGLHFTEELLAELGAKGVEIQRVTLHVGLGT
                     FEPVREERIDRHVMHREEYAIGAEAAAAIAAARTDPGRRVVAVGTTTVRTLESCWREH
                     GAIVAGRGETGIFLKPGDEFGVVDALVTNFHLPGSTLLMLVAAFAGREQVLAAYGEAI
                     RERYRFYSYGDAMLIE"
     gene            complement(2181..3281)
                     /gene="ftsZ"
                     /locus_tag="PLS90_12320"
                     /pseudo
     CDS             complement(2181..3281)
                     /gene="ftsZ"
                     /locus_tag="PLS90_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
     gene            complement(3503..4834)
                     /gene="ftsA"
                     /locus_tag="PLS90_12325"
     CDS             complement(3503..4834)
                     /gene="ftsA"
                     /locus_tag="PLS90_12325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPK03231.1"
                     /translation="MLFTRSALNFPAGPVQPFAPAGSKGRSRNLKLIKRHTRRRQKTR
                     LITGLDLGTTKVCAIIGNVEEDGSVNILGIGSSPSRGIRRGVVTDIGLTVEAIRGAYE
                     QAYNLARVHPRELLVGIAGDHICGLNREATIEVRNPEAGIDRRDCMRARDKALKVVMP
                     ATDEILHRFLKEYVVNEQPGIQNPVGLFGHNLQVRMHIVTCSTAAANNLFRCIRRAGL
                     KTSSVVLQSLASAQAVLTERERELGVVLVDIGGGTSDIAIFHNNILHHISEIAIGGDM
                     ITQDIAKILRCTPHDAENLKKKFGHAVPLSVDADERIELPRPNHHHQRVSYSRRELAE
                     IIEARVEDILVLVQQQIENSRVADQLYGGVVLTGGAALLEGITEVAERMLNCPCRIGK
                     PSGLLGMAGVASTPIYATGVGLLRWAAEEGPGYQREPWLVRKTKALFDIYG"
     gene            complement(4990..5808)
                     /locus_tag="PLS90_12330"
     CDS             complement(4990..5808)
                     /locus_tag="PLS90_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPK03232.1"
                     /translation="MSRPSQPRTGPSGRTFRESFLSRAGRLIRRVLTLCLLAGCVAGG
                     YFIALRVHDFVYASDYFKVREVRIGGASTALEAEARAWIEQRFAQDGDNLCRLDMDQL
                     ARELTALPRARRAEARRRFPDALLISFVEREPEAVVNLDRPHLIDRDGVILARADPGE
                     VSGLGLPVITGIRNGAWQPGDRILQRGLDEILEAARFVRTADSRIDRLIVEWNLDGRD
                     EVSAILRGGTQVLFGDIPPLHLLDKLSTALTAREDLTRARYIDLRMQKQVVFKM"
     gene            complement(5998..6927)
                     /gene="murB"
                     /locus_tag="PLS90_12335"
     CDS             complement(5998..6927)
                     /gene="murB"
                     /locus_tag="PLS90_12335"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPK03233.1"
                     /translation="MTDVNGQLEGLEHIDGVTVSAGEPLAKHVSMGVGGPARWFITVE
                     TVEALEAVLARLRGGAVPWMMLGGGSNTIFTEAGFGGAIVTLGRGFRQIAAGPGEHEV
                     TAGAGANLSALMNYAKRRGLTGMEWAAGVPGTVGGALAGNAGTGLGEMCDRVAAVEVI
                     DAAGARRMRRRGEFSYGYRRSDLARDVILRATVALAPDDVEAIQARIDQGLKKRFEQP
                     VGKRCSGCMFKNPAGDYAGRLIDRAGLKGLRVGGAQVSEEHANFIINEGEATSEDIRA
                     LVEEVRRRVREATGVELELEIRMVGLDSRLVLH"
     gene            7215..>7593
                     /locus_tag="PLS90_12340"
     CDS             7215..>7593
                     /locus_tag="PLS90_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03234.1"
                     /translation="MVHRRALGGCVALVACSILIGCAREPVPVATPRDLTEQRLLTPD
                     PTILVPNLAEIARLQPASAASSPAEITPPQEQSATEAERYRLRPGDLVEVLYRFPAAA
                     TAEPYRIGVGDQVRVDFVQTPEYD"
BASE COUNT         1108 a         3007 c         2094 g         1384 t
ORIGIN      
        1 cccttctcga atatccgtct ctcgccctca atctaaatcg accctcgtct cttgtcctcg
       61 tattcgcccc aaaggcgtcc ctcaagaatt cggctctcgg ctctcgtccc tcgtcctcgg
      121 ctctcgaaat cccgaatcct gtccctgatt aatgccgcag ccgcgaaacc acttctaatt
      181 ctaattgaat cgacttcttc acttgttctt gccttcttcc tctctctcct ctcttcttca
      241 ccttttctct cctccatccc ccctccgcct gctctcccat tctctctcac tcaatcagca
      301 tcgcatcgcc atagctgtaa aaccggtagc gctcgcggat cgcctcgccg taagccgcca
      361 gcacctgctc ccgtcccgcg aacgccgcga caagcatcag caacgtcgac ccgggcaggt
      421 gaaaattcgt cacgagggca tccacaacgc cgaattcgtc gcccggcttc agaaaaatcc
      481 cggtctcgcc ccgccccgcc acaatcgcgc cgtgttcccg ccagcacgac tccagcgtcc
      541 gcacggtcgt tgtcccgacc gccaccaccc gccgccccgg atccgtgcgc gccgccgcga
      601 tcgccgccgc ggcctccgcg ccgatcgcgt attcctcgcg gtgcatcacg tggcggtcaa
      661 tccgctcctc ccgcaccggc tcgaacgttc ccagccccac atgcagcgtg acgcgctgaa
      721 tctccacccc cttcgccccc agctccgcga gcagctcttc cgtgaaatgc agtccggccg
      781 tcggcgccgc caccgaacct tgcgcgcgcg cataaaccgt ctggtagcgc tccgtgtcct
      841 cccccgcggc cggcgcctcg cctcgcgcct tgcgctgttg cacgatgtac ggcggcaacg
      901 gcgcctcgcc cgcacgttcc agatagtcct ccacatcccc ctccacgtgc agcgcaacca
      961 cccgcgttcc gtccggcccg tcgcgcagca cccgcgcttc gcccccgccg ggcagatcca
     1021 gccgcgcgcc cggtttcagc ttccgggccg gccgcgccag cgcttcccag gtgccgtcgc
     1081 cgagcgagcg cagcagcagc agttccgccg cgccgccacc cgcgcgccgt gcccgcagcc
     1141 gcgctggaat cacccggctg tcgttaagca cgaggcaatc gcccggccgc agcagcgcgg
     1201 acagatcccg gaaccggtga tggcttatcg cccccgtccg ccgcgccagg acgagcaatc
     1261 gcgagtcgtc gcgccgcgcc gccgggtgcg tcgcgatcaa ttcctccggc aattgatatt
     1321 gaaaatccgc caattgcatg gcttcgctgc ctgttcgtcc cccctgtaac tcgtccctcg
     1381 tctcctgtcc ttcgtcctca ctcgaaaatt tgtccgcgat tctcggaatc ctggatcggt
     1441 tctgccgtcc ctacgggacc gcaggttcat gagcaactcg taatcgcgcc catcggcccg
     1501 ccccagaatt cggctctcgg ctctcgtccc tcgtcctcgg ctctcggaac ctgtatcgca
     1561 agtccctcag caactccttt gttcttcccg gcattccttc ttcccttgtt ttccactttc
     1621 ctcccgctct ccaccgcccc ccaaaacgca acgggggaga gcctttcgcc gctctccccc
     1681 gtctcacagt cactctggct ggtcggatct tactcgaaga agcgcggctt gcgacgacgc
     1741 tcgagagccg gaatgtcaaa cgggtcgctc tcggcatgct cgtcatcgcg cgcggcggcc
     1801 tcgtcggccg aatcacgcag caccgaaccc gaagccggct cggccggcgt ggtcggccgc
     1861 tgcgggaaca cggtccgcgt cggctcgccg ctggtccgcc cgaagctgaa tcggccgccc
     1921 gactcagcgg cccgtccccc cgcctctccg gcccggtgcg cggtttcccg cgccgactcg
     1981 cggtcgacac gctccaccgc cgccggtccg gccgcctcgc gccgcggcgc ttcttcccgc
     2041 cgggtggttt cctccgtcgc ctggctcgcc gaacgcgctt caatcgcccg ggtcaccgaa
     2101 ctcgtatgcc cgcccggcgc cggcgcttcg cgctcggccg gtttctccca cgccttcagc
     2161 ggattgtccg gccggttgaa gtccaccgtc gccggctgag cctgcgactg ttcgcgctcc
     2221 ttcttgcgcg tgatcgccgg cgtcgggtcg tgctcccggt cctcgcgacc ctccgccggc
     2281 cgctcatgca gcgaggacat gctgcggcgg ggccgcgcgg cccccggggt aaagccgctc
     2341 acgttcgatt ccatcgactc ggcaaaacca gtcgcgatga tcgtgacgcg catctcgtcg
     2401 cgcatgctct cgtcgatcac aacgccgaag ataatgttcg cctccgggtc ggccgactcg
     2461 tagatcagcg acgtcgcctc ggtgatctcg tgcatcgtga cgtccggccc gccggtgatg
     2521 ttcatcagca cgccgcgcgc cccgtcgatc acgatcttct cctgcagcgg gctgtggcac
     2581 gccttgcgca ccgcttcgac ggcgcggttc tcgcccttgc ccacgcccac gcccatcacc
     2641 gccccgccgg tctcgcccat gatcgtccgc acgtcgcgga agtcgacgtt gatcagcccc
     2701 ggcatgctga tcaggtcgct gatcgcccgg atcccctggg ccagcacgct gttggcaatc
     2761 tgaaacgctt cggtcagcgg cgtcttcggt cccaccgtct ccatcagctt gtcattcatg
     2821 atgacaatca tggtgtcgac gttttccttc agccgccgga tgccggcctc ggcgcggcgc
     2881 atccgctggg gcccctcgaa tgtgaagggc ttggtcacga tcgccacgct caggatgccc
     2941 atctccttgg catactcggc gacgaccggc gcggcgccgg tgcccgtgcc gccccccatt
     3001 ccggccgtga tgaagaccat gtcggccccc tcgagggccg ccttcagctc ggcctcgtgc
     3061 gcgcgcgcgg cctcctcccc cagctccgga atcgccccgg cgcccaggcc gtgcgtgatc
     3121 tcctcgccga tctggatctt gttgcccacc tggcagcgct gcagcgcctg gaaatcggtg
     3181 ttgatcacaa agaagtccac accgccgaag ctcttgctga ccatatcctc gacggcattg
     3241 cagccgccgc cgcccacgcc gatcaccttg atccgcgtca tcgtcgcggt ttcttcatcc
     3301 cattggatct tcgccatccg tcgctccgtt cgcggggtcc tcccccgggg ctgttccagc
     3361 tcgggctgct cccagatgac ggccacctcg ccgtttccct gggggcgcag gggcgcctcg
     3421 tctcgccgcg catactcgtg cctggtctcc atctgccgtc acctgttccc ttccggcacg
     3481 atcccttaga tcgtgccatg gtttacccgt agatatcaaa cagtgctttt gttttacgca
     3541 ccagccacgg ttcgcgctgg tagcccggac cttcttcggc ggcccaccgc agcaggccga
     3601 cacctgtggc gtagatcggc gtgctcgcca cccccgccat ccccagcagc cccgacggct
     3661 tgccgatccg gcacgggcag ttcagcatcc gctcggcgac ctcggtgatc ccctccagca
     3721 gcgccgcccc cccggtcagc acgaccccgc catacagttg gtcggcgacg cgcgagttct
     3781 cgatctgctg ctgcaccagg accaggatat cctccacccg cgcttcaatg atctcggcca
     3841 gttcgcgccg gctgtagctc acgcgctggt ggtgatgatt cggccgcggc agctcgatcc
     3901 gctcgtccgc atccacgctc aacggcacgg catggccgaa tttcttcttc aaattctccg
     3961 cgtcgtgcgg cgtgcagcgc agaatcttgg cgatatcctg cgtgatcatg tcgccgccga
     4021 tcgcgatctc gctgatgtga tgcaggatgt tgttgtggaa gatcgcgatg tccgacgtcc
     4081 ccccgccgat gtcgaccagt accacgccca gctcccgctc ccgctcggtc agcaccgcct
     4141 gcgcgctcgc cagcgactgc aagaccaccg acgaggtctt cagccccgcc cgccggatgc
     4201 agcggaacag attgttcgcc gccgccgtcg agcacgtgac gatgtgcatc cgcacctgca
     4261 gattgtggcc gaacagcccc accgggttct gaatccccgg ctgctcgttg accacatact
     4321 ccttcaaaaa gcggtgcagg atttcgtccg tcgccggcat caccaccttg agcgccttgt
     4381 cgcgcgcccg catgcagtcg cgccggtcga tccccgcctc ggggttgcgc acctcgatcg
     4441 tcgcctcgcg gttcaagccg cagatatgat cgcccgcaat ccccaccagc aactcgcggg
     4501 ggtggacgcg cgccagattg taggcttgct cgtaggcgcc gcggatcgcc tcgaccgtca
     4561 acccgatgtc ggtcacgacg ccgcgccgga tgccgcgcga gggcgacgac ccgatcccca
     4621 ggatgttgac gctcccgtct tcctccacat tgccgatgat cgcgcagacc ttcgtcgtcc
     4681 ccaaatccag gccggtgatc agccgcgttt tctggcgccg ccgcgtgtgg cgcttgatca
     4741 gtttcaggtt gcgactgcga cccttgctgc ccgccggcgc gaacggctgg accggccccg
     4801 ccggaaaatt cagtgcactg cgcgtgaata gcatcgttcc agttccacct tcctgcttgc
     4861 tccgacggtg tctgcgtccc acctcaaaat ccgtctctct tccctcgtct ctcgtcctcg
     4921 tccgcgtaat cgcccccgac ggccagccca ggaattcctc cccgtctccg aacggcacct
     4981 accccgggat tacatcttga acaccacctg cttctgcatc cgcagatcga tgtaacgcgc
     5041 acgcgtcagg tcctcgcgcg cggtgagcgc cgtgcttagc ttgtcgagca gatgcagcgg
     5101 tgggatgtcg ccaaagagga cctgcgtgcc gccgcgcaga atcgccgaga cctcgtcgcg
     5161 gccgtccaga ttccactcga cgatcagccg gtcgatccgc gaatcggcgg tccgcacgaa
     5221 ccgcgccgcc tccagaatct catccaggcc gcgctgcaga atccggtcgc ccggctgcca
     5281 cgcaccgttg cggatgccgg tgatcaccgg caggcccagg ccgctcacct cgcccgggtc
     5341 ggcccgcgcc agaatcacgc cgtcgcgatc gatcaggtgc ggccggtcca ggttgaccac
     5401 ggcctccggt tcgcgctcca cgaacgagat cagtaacgca tcggggaaac ggcggcgcgc
     5461 ctcggcccgc cgcgcgcgcg gcaacgccgt cagctcgcgc gccaactggt ccatgtcgag
     5521 ccggcaaagg ttgtcgccgt cctgggcgaa acgctgctcg atccacgcgc gggcctcggc
     5581 ttccagcgcc gtggaggccc cgccgatgcg cacctcgcgc actttgaagt aatccgatgc
     5641 ataaacgaaa tcgtggacgc gcagcgcgat gaagtacccg ccggccacgc acccggccag
     5701 caggcacagc gtcagaaccc gccggatcag gcgtccggcg cgcgacagga aggattcgcg
     5761 gaacgtacgg cccgacggtc ccgtacgcgg ttgggaaggc cggctcatct ggctgctcgc
     5821 tgcgtccgcg gattctgctc gcgctggccc cgccggccgg ctgggctctc gcgcatcact
     5881 gctacaacgg gatcaggaac tcaaacaacg gcgggctcac gtctcccgcc caacgaaacc
     5941 ggggacgaac caaacaacgc gcgcgatccg ccccggtctg acgaatgtct gggaatccta
     6001 gtgcaacacg agacgagaat caagccctac catgcggatt tccagttcca attcaacccc
     6061 ggtcgcctcc ctcacgcgac ggcgcacttc ctccaccagc gccctgatgt cctcgctcgt
     6121 cgcctccccc tcgttgatga tgaagttcgc gtgctcctcg ctgacctgcg cgcctcccac
     6181 gcgcaacccc ttcagccccg cccggtcgat cagccgcccg gcgtaatccc cggccgggtt
     6241 cttgaacatg cagcccgagc agcgtttccc caccggctgc tcgaagcgct tcttcagccc
     6301 ctggtcgatc cgcgcctgga tggcctcgac gtcgtcgggc gccagggcca cggtcgcccg
     6361 caggatcacg tcgcgcgcca gatcgctgcg ccggtaaccg tagctgaatt ccccgcgccg
     6421 gcgcattcgc cgcgcgccgg cggcgtcgat cacctccacc gccgccaccc ggtcgcacat
     6481 ctcccccagc ccggtgccgg cgttgcccgc cagcgcgccg ccgaccgtcc ccggcacccc
     6541 cgcggcccac tccatcccgg tcaggccgcg ccgcttggcg taattcatca gcgccgacag
     6601 gttggccccg gcccccgccg tcacctcatg ctcgcccggg ccggccgcta tctggcggaa
     6661 gccccgcccc agcgtgacga tcgcgccccc gaagcccgcc tcggtgaaga tcgtgttgct
     6721 gccgcccccc agcatcatcc acggcacggc gccccctctc agtcgcgcca gcaccgcctc
     6781 cagcgcctcc acggtctcca ccgtgatgaa ccaccgcgcc ggcccgccca cgcccatcga
     6841 aacgtgtttc gccagcggtt cccccgcact caccgtcacg ccgtcgatgt gctccagtcc
     6901 ctcgagctgt ccgttcacgt ccgtcatctt aaccgccatc gtggagcggg cttcccgccc
     6961 gtcggaattc aattcgcgca tcttatcgca acccatctca agatgcctgc cctaaagcca
     7021 accaagaatc ggtagcggca tcatcggtat cgccatcact gtcccccagc caacaaacct
     7081 gaaacccctt gagttgcagc gccgttcttc atgtagcgtc ccaggctttt ttgctttcaa
     7141 gccgccccgc cctctgctat tccatgggcg ttgctcatgg ctggttccac ttgccgaccg
     7201 aaagagggct ccccatggtt caccgccgag cgctgggcgg ctgtgtcgcg ctggttgcct
     7261 gttccattct gatcggctgc gcgcgcgagc cggtccctgt cgcgacgccg cgggacctca
     7321 ccgagcagcg cctgctgacc cccgacccga cgatcctggt gcccaacctc gcggagatcg
     7381 cccgcctcca gccggcgagc gcggcgtcct ccccggcgga gattaccccg ccccaggagc
     7441 agtccgcgac cgaggccgaa cgctaccgcc tccggccggg cgatctggtc gaggtcctct
     7501 atcgcttccc cgccgccgcc accgccgaac cctaccgcat cggcgtcggc gaccaggtgc
     7561 gcgtcgactt cgtccagacc ccggagtacg acc
//
LOCUS       DAOPCO010000192         7559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8813_length_7559_cov_5.317031, whole genome shotgun sequence.
ACCESSION   DAOPCO010000192 DAOPCO010000000
VERSION     DAOPCO010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_7559_cov_5.317031"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PLS90_12345"
     CDS             <1..502
                     /locus_tag="PLS90_12345"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPK03235.1"
                     /translation="VVPLHLPPLRERRDDIAPLVRHFINEFNVRFGKRIEGVAPEALA
                     LLEAHAWQGNVRELENVIQRAMVMSAGNVINPAALPPGLGANGATAAPEPAGNPGDPP
                     PELPDRPLTEYVEEIVEREERRLIEAALRKTGGRRQETADLLGIARKSLHNKMKRYGM
                     LDQDEE"
     gene            complement(1183..2091)
                     /gene="tsf"
                     /locus_tag="PLS90_12350"
     CDS             complement(1183..2091)
                     /gene="tsf"
                     /locus_tag="PLS90_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPK03236.1"
                     /translation="MAEITASMVKELREKTGAGMMDCKKALAECDGDMERAVDELRKR
                     GQAVADKRGGRATREGLVFERTEGAVGVLVELNCETDFVARNDEFRAIGRDLADLLFQ
                     HGDQFTDPSQLMGFTSPEHGRTVSEILTEAIGKTGENMGVRRFVRLNADQNGPHGFIT
                     SYIHPPGKLGVLVEVHAGKPESLNNAGVRQLGRDLAMHIAAAAPVSISRNDVDPALVA
                     REKKIYMEQAQHEGKPEKIWEKIAEGKLAKFYKQSCLLEQEFVKDPDQTIEQLVAAVG
                     KAAGDTLTVARFERFEVGAGAEQEEA"
     gene            complement(2253..3104)
                     /gene="rpsB"
                     /locus_tag="PLS90_12355"
     CDS             complement(2253..3104)
                     /gene="rpsB"
                     /locus_tag="PLS90_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPK03237.1"
                     /translation="MLGQISMKQLLEAGVHFGHQRRRWNPKMKKYIYTERNGIYIIDL
                     KKTLRLLREAYSFVRDTTFNGGTGIFVGTKKQARDAIERWAHFAGCHYVNNRWLGGTL
                     TNFETINRSIKRMIELQELCGSGQIERYSKKEQSQIIREKESLEKNLQGIREMQKRPD
                     FIFVIDPSKETIAIREARKVGVTVVAVVDTNCDPDPIDLVIPGNDDAIRSINLSCEKM
                     AEAILEGKMRRVEAGLDPMEVLPEQARQLLAQQREHGEAEEGGYDEGGQAADDYAEET
                     AGAEEAE"
     gene            complement(3535..4224)
                     /locus_tag="PLS90_12360"
     CDS             complement(3535..4224)
                     /locus_tag="PLS90_12360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03238.1"
                     /translation="MTHTSTPSLPRAAVTLLELLVVVLIISILATIATGVYTGEAERA
                     RIAATQDLIHQLEVAITRYEADLGQFPPSGSGDVDGSLINLRDGSGFLQLALVHSMAG
                     SVYNAPPQWRGPYINVKSINLDPTATVAGEFDILDAWGSPLVYVTSDFYESRGPSFTG
                     GTVMFSSTRPDGANPDLPAPNPFVERGETYYNSSTYQIYSPGPNGKTLGVTSEGQFAG
                     TEADDINNFGY"
     gene            complement(4276..5064)
                     /locus_tag="PLS90_12365"
     CDS             complement(4276..5064)
                     /locus_tag="PLS90_12365"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03239.1"
                     /translation="MNDSRRRGNAGFTLTELLVVIVIISILSTIALPVYVSRAEDARI
                     AAAKAEVKELAMAEEQVAIHHGFYVPLQLLDDIAGGQGVADINSTDDAIGNESTTIRL
                     IDVNQPIDRLDINQPTLADTDLIRVRRLIEDWQGPFMSFQRVNIGDSDTRDPDQLNPT
                     LIQYDHPLDPWGQPYRFYSPVGIIGSNALTAFRPDEWSSADFSDGTVTNTDDRFDRFA
                     IVSYGPDSVPGDYVLGNVSDDIFYEFGSIVLPRNETGFSVEGRR"
     gene            complement(5233..6081)
                     /locus_tag="PLS90_12370"
     CDS             complement(5233..6081)
                     /locus_tag="PLS90_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03240.1"
                     /translation="MSHRPKSPIREPRRPAGFTIVEVIVTFIIIGILATILVPVLVNR
                     AEQARIASAEQELSHLKDAMERAAIDTGYFFRLDVLDDGPGEDGIAATAPRVTPGVKD
                     RIEGILDNDVTTDNLYDEPEKIFIIPSTQDFHPLYATQLFPRLEENETNFNWRGPYIN
                     YRRDANGNDWPDDPWGNDYLFFTRAGILYPPLADPGDPTWGCDSFQDQGPAMVVTDAN
                     GATTVERFPVDAPLFDRPTILSMGPDGMPGDGTSPPDNNYGQGDDIYVQFGGQYRDPN
                     NFSNGR"
     gene            complement(6119..6877)
                     /locus_tag="PLS90_12375"
     CDS             complement(6119..6877)
                     /locus_tag="PLS90_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03241.1"
                     /translation="MRRYQQRHGRGRRGFTAIEISAVAAIIAILTLILVPIVRNQVAK
                     SKIVAAQDDMFSIAKGTEISFAETGFTVRPHDLLRGPDQEASATIESPVMEWNRPLQL
                     TQIAQLARTWDGPYVTVNRSEPLPVLVLNRGVAFRTPNGVGGGPILLRDDDEYLEPDD
                     FGQFPQYPVDPWGNPYILFGPGALGNVGSPDIDPIYRESEFNSGMIYSLGPDGLPGNM
                     NGQDPRHYYPDRYKDPNDPTYGGLGNGDDLSRSF"
     gene            complement(6894..>7559)
                     /locus_tag="PLS90_12380"
     CDS             complement(6894..>7559)
                     /locus_tag="PLS90_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03242.1"
                     /translation="LPATLVIFPTGVYPSFDMYGQFPPNNVSLIANVRENWNGPYIGT
                     SEARNRQAQGGRGLVTVRLSDFSRGVTLPGIGDASLVQWPTDTWGRPWVVYQVTTDFN
                     LVSAQNPAGIRWISSPTELANYFNAVVSYGPNGVPGGLPPGGPYFTTEQDLATLTRHR
                     LFTDGDLYGTGAFYTMKSLVATGEAALPAIEDLAYSLNTRSNDEGEVGILDPGSDDII
                     FRF"
BASE COUNT         1242 a         2565 c         2320 g         1432 t
ORIGIN      
        1 cgtcgtgccg ctccacctgc cgccgctgcg cgaacggcgc gacgacatcg ccccgctcgt
       61 ccgccatttc atcaacgagt tcaatgtgcg cttcggaaaa cggatcgagg gggttgcgcc
      121 cgaagccctc gcgctgctcg aagcccacgc ctggcagggc aacgtccgcg aactggagaa
      181 cgtgatccag cgcgcgatgg tgatgagcgc gggcaatgtg atcaacccgg cggcgctgcc
      241 gcccggcctg ggcgccaacg gggcgaccgc cgcgccagaa cccgccggga accccggcga
      301 ccctccgccg gaactgcccg atcgcccgct gacggaatat gtggaggaga tcgtcgagcg
      361 cgaggagcgc cgactgatcg aagcggcgct gcgcaagacc ggcggccgcc gccaggagac
      421 cgccgatctg ctcggcatcg cccgcaagag cctgcacaac aagatgaaac gctacggcat
      481 gctcgaccag gacgaggagt aacccgcccc tcgccgggat cgtccctcgt cctcgtcgtc
      541 gtgaacgatc ctccccggcc gttgccttcc tccgcgacgc ctcatgcaaa gaagccaacc
      601 gaccgaaaat tggaaaccgg ggagcgccac tctctcctga gtggcaatgc ccccattttc
      661 ctcccccgct gtgaacggcc tgttcatgag caactcgtaa tcgatcgttc ctccgttcct
      721 cttggccaaa acaaacaccg gaagaaatca gaaaagcggt gctcaacgca aaggcgcaaa
      781 gcacatacta tgcttggggc gggcttgtcg cttggtctgt gcaggcatca gccgaaattc
      841 tcgccgtggt ggttgtgggc tgtctgcgcg gtgctggcag gcgtcggttc gttcctgccg
      901 ctcggtgcgt tgcccggcga tttgcaggca gagtccggaa cgggattcac aatctacgca
      961 tccggcccat caatcgatga acagttggat tcatggatgc ttgatctggc gcggcgaatg
     1021 ggactgcgat gagcatcctc gcccagcatc gcatggaacc atgatgcttt gtaagcaaag
     1081 agccggcggg cttcgcttcg gcaggctacg gtcgaagagg caaagaaaag ccggcccaac
     1141 ttgcgccggg ccggccgttc atactggtat caggttttcg gctcaggctt cctcttgctc
     1201 ggcgccggcg ccgacctcga accgttcgaa ccgggcgacc gtcagcgtgt cgcccgcggc
     1261 cttgccgacg gcggcgacca actgctcgat cgtctgatcc gggtccttga cgaactcctg
     1321 ctcgagcagg cagctctgct tgtagaactt cgccagtttg ccctcggcga tcttctccca
     1381 gatcttctcg ggcttgccct cgtgctgcgc ctgctccatg tagatcttct tctcgcgcgc
     1441 gacgagcgcc ggatcgacgt cgttgcgcga gatcgagacc ggcgccgccg ccgcgatgtg
     1501 catcgccagg tcgcgcccaa gctgacgcac cccggcgttg ttcagcgatt cgggcttgcc
     1561 ggcatgcacc tcgaccagta cgccgagctt gcccggcggg tggatgtacg acgtgatgaa
     1621 gccatgcggg ccgttctggt cggcattgag ccgcacgaac cgccgcacgc ccatgttctc
     1681 gccggtcttg ccgatcgcct cggtcaggat ctcgctgacc gtgcggccgt gctcggggct
     1741 ggtgaagccc atcagttgcg acggatcggt gaactggtcg ccgtgctgga acagcaggtc
     1801 ggccaggtcg cgaccgatcg cgcggaactc gtcgttgcgc gcgacgaagt cggtctcaca
     1861 gttcagctcg accagcacgc cgacggcccc ctccgtccgt tcgaagacca gaccctcgcg
     1921 ggtggcgcgg ccgccgcgct tgtcggccac cgcctggccg cgcttgcgca gctcgtccac
     1981 cgcccgctcc atgtcgccgt cgcattcggc cagcgccttc ttgcaatcca tcatgcctgc
     2041 gccggtcttc tcgcgcagtt ccttgaccat gctcgcggta atctctgcca ccgtcctgct
     2101 cctctcggca ttcgtcggtt agttcgtccc tcgtcctcgc cctcggctct cgaaacctcg
     2161 agaccaaaca gtcgggcgga cggcgaggcg gggcgctgcg ccggccggcc gtttgcgggt
     2221 cagcctggcg ccttgcgggt tgatttcggt cgttactcgg cttcctcggc gccggcggtt
     2281 tcctcggcgt aatcgtcggc cgcttggccg ccctcgtcgt agccgccctc ctcggcctcg
     2341 ccgtgctcgc gctgctgcgc caggagctgg cgcgcctgct cgggcaggac ttccatcgga
     2401 tcgagaccgg cctccacgcg gcgcatcttc ccctcgagga tcgcctcggc cattttctcg
     2461 cacgacaggt tgatcgagcg gatcgcatcg tcattgcccg gaatgaccag gtcgatcgga
     2521 tcgggatcac agttcgtgtc gacgacggcg acgaccgtga cgccgacctt gcgcgcctcg
     2581 cggatcgcaa tcgtctcctt gctcgggtca atcacgaaga tgaagtcggg ccgcttctgc
     2641 atctcgcgaa tcccctgcag gttcttctcc aggctttcct tctcgcggat gatctggctc
     2701 tgttccttct tgctgtagcg ctcgatctga cccgagccgc agagctcctg cagctcgatc
     2761 atgcgcttga tcgaccggtt gatcgtttcg aaattggtga gcgtgccgcc cagccagcgg
     2821 ttgttgacgt agtggcagcc ggcgaagtgc gcccagcgct cgatcgcgtc gcgcgcctgc
     2881 ttcttggtgc cgacgaagat gccggtgccg ccgttgaacg tcgtgtcgcg cacgaagctg
     2941 taggcttcgc gcagcagccg gagcgtcttc ttcaggtcaa tgatgtagat cccattgcgc
     3001 tccgtgtaga tatacttctt catcttgggg ttccagcgac ggcgctgatg gccgaagtga
     3061 acacccgcct cgagcaactg cttcatgctg atctggccaa gcatgatcgg gtcctccgtg
     3121 ggacgtgggg ttgagcctcc gccccggctc ctggtgcgcg acgcggcgcg cacggtcagg
     3181 tgcgatcggc tgcaaacggc cgtccgggac cgtttcaccc cgccgatccg ccggtggcgt
     3241 gtgaagtggg gagagccgcc ttcggaggga ctctccggcc gggcctcgcg gccccgcaaa
     3301 cgattgttgg agtggcactt aggctgccca gcgaccgcag gcgggggaga cgcgggtctc
     3361 cccggcacgc acacaatggc ggcctactgt agcaaaacgg ctccgcggcg ggcaagttgg
     3421 aaaaggatcc cggccccgta ggaggtatcg gtatcgcttt cggccgcgaa tcgcaaaacg
     3481 ccgcgagcca tccgcggctc atcggcagcc tccggcggct taatgcggcg cgactcaata
     3541 accaaaattg ttgatatcgt ccgcttccgt gcccgcgaac tgcccctccg acgtcacgcc
     3601 gagggtcttc ccattgggcc cgggcgaata gatctgatac gtgctgctgt tgtaatacgt
     3661 ctcgccccgc tcgacgaacg ggttgggcgc cggcagatcg ggattcgccc cgtcgggccg
     3721 cgtcgaactg aacatcaccg tgccgccggt gaacgagggc ccccgcgact catagaaatc
     3781 actggtgaca tagaccagcg ggctgcccca ggcgtccagg atgtcaaatt cgccggcgac
     3841 ggtcgccgtc gggtccaggt tgatggactt gacgttgata tacggcccgc gccactgcgg
     3901 gggggcgtta tacacgctgc cggccatgct gtggaccagc gccaactgca gaaatcccga
     3961 cccgtcgcgc agattgatca gcgaaccatc cacatcgccc gaaccgctcg gcgggaactg
     4021 acccaggtcg gcctcatagc gcgtgatcgc cacttcgagc tgatgaatca ggtcctgcgt
     4081 cgccgcgatc cgcgcccgtt cggcctcgcc ggtatagacc cccgtggcga tcgtcgccag
     4141 gatgctgatg atcagcacca cgaccagcag ttccagcagc gtgaccgccg cgcggggcag
     4201 ggacggggtg gatgtgtgtg tcacgtgcct cagactcctg tgtcagaatc gcctggtccg
     4261 cgcgcgccgg ctggtttacc ggcggccctc gaccgagaag cccgtctcgt tgcgcggcag
     4321 gacgatcgaa ccgaactcat agaaaatatc gtccgacaca ttgccgagaa catagtcgcc
     4381 cggcaccgag tccggtccat acgacacgat cgcgaaacga tcaaaccggt catccgtgtt
     4441 cgtcacggtg ccgtcggaga agtccgcgct cgaccattcg tccggcctga acgccgtcag
     4501 cgcgttggac ccaatgatcc ccaccgggct gtagaaccgg tagggctggc cccacgggtc
     4561 cagcgggtgg tcatactgaa tcagcgtcgg gttaagctga tccggatcgc gcgtgtcgga
     4621 gtcgccgatg ttgacccgct ggaacgacat gaacgggcct tgccagtcct cgatcaggcg
     4681 ccgcacgcgg atcaggtcgg tgtcggccag cgtcggctga ttgatatcga gccggtcgat
     4741 cggctggttg acgtcgatca gccggatcgt cgtcgactcg ttgccgatcg catcatcggt
     4801 cgagttgatg tcggccacgc cctggccgcc ggcgatgtcg tcgagcaact gcagcgggac
     4861 gtaaaagccg tggtggatcg ccacctgctc ctccgccatc gccagctcct tgacctcggc
     4921 cttcgccgcc gcgatgcgcg catcctcggc ccggctgacg tataccggca gcgcaatcgt
     4981 cgacaggatg ctgatgatca caattaccac cagcaactcg gtcagcgtaa agcccgcgtt
     5041 cccacgtcgt ctgctgtcgt tcatgttccg ccgtctccca gcgccgccgt gcgcggccga
     5101 tgatcgcgga ccggggaaac ttccccccgg cccttcactc aagtcttgtc cgttcgtcct
     5161 cgtcctcggc tcttccaatg ccgccaaact tttcctggcc tttcacgttg cgcccgcggc
     5221 ggcctccgcg cgtcatctac cgttcgaaaa gttgttcgga tcgcgatact gtccgccgaa
     5281 ctgcacgtag atatcgtcgc cctgcccgta gttgttatcg ggcggcgacg tgccgtcgcc
     5341 cggcatcccg tccggaccca tcgacagaat cgtcggccgg tcaaacagcg gcgcatcaac
     5401 cgggaagcgc tccacggtcg tcgcgccgtt ggcgtcggtc accaccatcg ccggtccttg
     5461 gtcctggaac gaatcgcagc cccaagtcgg gtcgcccgga tcggccagcg gcggatacag
     5521 gatccccgcc cgcgtgaaga acagatagtc gttcccccac ggatcgtcgg gccagtcgtt
     5581 gccgttggcg tcgcgccggt agttgatata tggcccccgc cagttgaagt tggtttcatt
     5641 ctcctccagc cgcgggaaga gctgcgtggc atagagcggg tggaaatcct gcgtgctcgg
     5701 aatgatgaag atcttctcgg gctcgtcata caggttgtcg gtcgtcacat cgttgtccag
     5761 aatcccctcg atccgatctt tcacgccagg cgtgacgcgc ggagccgtcg ccgcgattcc
     5821 gtcctcgccc gggccgtcat ccagcacgtc gagccggaag aaataccccg tatcgatcgc
     5881 cgcccgctcc atcgcgtcct tcaggtgcga gagttcctgc tcggccgagg cgatgcgcgc
     5941 ctgctcggcg cgattgacca gcaccggcac cagaatcgtc gccagaatgc cgatgatgat
     6001 gaacgtgacg atgacctcga cgatcgtgaa cccggccggc cggcgcggct cgcggatcgg
     6061 cgatttgggg cggtggctca tggttgcttc cttgcgcgtt gaccgcgtct cacgctcgct
     6121 agaatgatcg actcagatcg tccccgttgc cgaggccgcc atacgtcggg tcattgggat
     6181 ccttgtaacg gtccggataa tagtgccgcg ggtcctgacc gttcatgttc cccggcagtc
     6241 cgtccggccc cagcgagtag atcatcccgc tgttgaactc cgattcccgg tagatcggat
     6301 cgatgtcggg cgaccccaca ttgcccagcg ctcccggccc gaacagaatg taggggttcc
     6361 cccacggatc gaccggatac tgcggaaact ggccgaaatc atcgggctcc agatactcgt
     6421 cgtcgtcgcg cagcagaatc ggtccgcctc cgacgccgtt cggcgtgcgg aacgccaccc
     6481 cccgattcag cacgagcacc ggcaacggtt ccgaacggtt gaccgtcaca tacggcccgt
     6541 cccacgtgcg ggccagttgc gcgatctgcg tcagttgcag cggccggttc cattccatca
     6601 ccgggctttc gatcgtcgcc gaggcctcct ggtccggtcc gcgcagcaga tcgtgcggcc
     6661 gaaccgtgaa accggtttcc gcgaacgaaa tctccgtgcc cttggcgatc gagaacatgt
     6721 cgtcctgcgc cgccacgatt ttgctcttcg ccacctgatt gcgcacgatc ggcaccagga
     6781 tcagcgtcag aatggcgatg atcgccgcca cagcgctgat ctcaatcgcc gtgaagccgc
     6841 gtcgccctcg accgtgccgt tgctgatacc gtcgcatgct tgtcgcttcc atctcagaat
     6901 cggaagatga tgtcgtccga accggggtcc agaatgccga cttcgccttc gtcattcgaa
     6961 cgcgtgttga ggctgtacgc caggtcttcg atggccggca gcgccgcctc gcccgtcgcc
     7021 accagcgact tcatcgtgta gaaggcgccg gtcccgtaga gatcgccgtc ggtgaacagc
     7081 cggtgccgtg tcagcgtcgc cagatcctgt tccgtcgtga aatacggtcc ccccggcggc
     7141 agcccccccg gcacgccgtt ggggccgtaa ctgacgaccg cgttgaagta attggccagc
     7201 tcggtcgggc tgctgatcca gcggatcccg gccggattct gcgccgaaac cagattgaag
     7261 tcggtcgtca cctgatacac cacccacggc cggccccatg tgtcggtcgg ccactgcacc
     7321 agggaagcat caccaatgcc gggcagcgtc acgccgcggc tgaaatcgct cagccgtacc
     7381 gtcaccagcc cgcgcccccc ctgcgcctgg cggttgcgcg cttcgctcgt cccgatgtac
     7441 gggccgttcc agttctccct gacgttggcg atcaggctga cgttgttcgg cggaaactga
     7501 ccatacatat cgaagctggg gtaaacgccg gtggggaaaa tcaccaacgt cgcgggcag
//
LOCUS       DAOPCO010000193         7552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8818_length_7552_cov_4.633187, whole genome shotgun sequence.
ACCESSION   DAOPCO010000193 DAOPCO010000000
VERSION     DAOPCO010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8818_length_7552_cov_4.633187"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1228)
                     /gene="flhB"
                     /locus_tag="PLS90_12385"
     CDS             complement(110..1228)
                     /gene="flhB"
                     /locus_tag="PLS90_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPK03243.1"
                     /translation="MADHDQEKTEYRSQKKLEESREKGEIPRSTELGTFLVFALFLTF
                     FGIARLAWFEGIGRIMEDLLRFDRHLGAINRDTVGEFLLVPFIKAGAVVAPLFCTILV
                     LSPLVNFCQTGFNIARNKLQPDWGRLNPVNGFKRIFSARQYIEGLKACVKIGLFSLLA
                     WSALLKSVPKLKLLAGQDLTNQLQTMLDISLMIGVRVAILMGVLAVFDYGYQWWEFNK
                     KLLLTHQELKDEMKEREGNPMIKQRQRQIAMQRARRRMMAEVPKAAVIITNPTHYAVA
                     LAYDREKHAAPYVAAKGTELMAHRIREIARRHNIPIIENRPLARALYRKVKLGQTIPS
                     EFYKAVAEILAFVFLLKRNRKLGLPAPSVSKVGRVRTD"
     gene            complement(1274..2050)
                     /gene="fliR"
                     /locus_tag="PLS90_12390"
     CDS             complement(1274..2050)
                     /gene="fliR"
                     /locus_tag="PLS90_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPK03244.1"
                     /translation="MISIPLAPIYVGMLIFLRVGLVFLFFPLFGERFVPVRVRLLLGL
                     ATAIVLTPVAPVAVAAFPVTPWEFVQLVLMEALLAFGVGIIGRVLFGVVQFSGQIAGE
                     QMGFGLVNAIDPTGAQQISVVAELQYVLAILVFLAAELHHVFIGAIARSFEVLPPGGA
                     VLNAGVVDFVMRLGHVLFLLSVQFAMPVIIIIFCINVALGMVARAVPQINVFMESFPL
                     RIIAGVSMMMIMLSVSVGLWLGMFGDMEGMIGELMGLMRG"
     gene            complement(2047..2316)
                     /gene="fliQ"
                     /locus_tag="PLS90_12395"
     CDS             complement(2047..2316)
                     /gene="fliQ"
                     /locus_tag="PLS90_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPK03245.1"
                     /translation="MDPQVVLDLLQEAFQIALMLSLPVMATTLVVGVSISIFQSITSI
                     QEQTLVFVPKIVAVILAIIVCFSWMMNTVLNYTHDLFVLIPDLIR"
     gene            complement(2334..3161)
                     /gene="fliP"
                     /locus_tag="PLS90_12400"
     CDS             complement(2334..3161)
                     /gene="fliP"
                     /locus_tag="PLS90_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175150.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPK03246.1"
                     /translation="MRLRWLVLLPLLVLAPGLAAQPAGAGAAGGMATPPGMNLSALPS
                     IAGPSLTLSLNGAGGAAAESGNGAVAMGLQILALLTVLTLAPAILLMLTSFTRIVVVL
                     GFVRRALSTQEVPPTQVLIGLSLFLTFFVMAPTWTSVYEGAIRPYFNEEIALAEMIDR
                     AETPTREFLFENTRKNDLALFIRMARIERPQTKADVPTYVLIPSFIVSELQTAFLIGF
                     IIYIPFLIIDMVVSSVLLSMGMMMLPPVIVSLPFKIILFVLVDGWNLIIGSLVRSYF"
     gene            complement(3158..3697)
                     /gene="fliO"
                     /locus_tag="PLS90_12405"
     CDS             complement(3158..3697)
                     /gene="fliO"
                     /locus_tag="PLS90_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPK03247.1"
                     /translation="MLFIRIAKHRLVMAVLFGLLVLAGGAARGWAVTDQEIAALDYGG
                     PEKPGETAVADPPSLGRLMVQMTLAMGVVLGLIGGVLWAARRFLPRSMPGMRPGQIEI
                     LATRTLGQRRSLLLVRARDKTLLLGATPAAIQVLTEFEDEALAWSAAAERAGVEEAAA
                     HAGGTTSLERDRPSLEVFK"
     gene            complement(3773..4027)
                     /gene="fliN"
                     /locus_tag="PLS90_12410"
                     /pseudo
     CDS             complement(3773..4027)
                     /gene="fliN"
                     /locus_tag="PLS90_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
     gene            complement(4180..5160)
                     /gene="fliM"
                     /locus_tag="PLS90_12415"
     CDS             complement(4180..5160)
                     /gene="fliM"
                     /locus_tag="PLS90_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPK03248.1"
                     /translation="MANVLSQEEIDALLGGISGGQIDTSRNDGEDQEPEAVQAFDFSD
                     QDRYLRGRLPTLETIHDRFARLLRLSLSTILRRAVEIQVCSQTVCNFGDFSRCLEKPS
                     SLHTLKLEPLKGSGLLVLPARLIMTLVDLLFGGDGKPFDVREDRDFTLIESRVIVKMA
                     EDICRAYTESWAPVRPLTVTVGSSETNILFVNIAQPNDPVTVVDCDVVIEGTRSSLSI
                     CIPYATIEPIKDILKGTFIGDPLDMDNAAAQRMRDLLLDSTVELRAMLGTATITGREL
                     LQMKAGDIIQLNEDYEHPVEILVEGERKFWGVIGAHKSTRALQITGTVEA"
     gene            complement(5182..5745)
                     /locus_tag="PLS90_12420"
     CDS             complement(5182..5745)
                     /locus_tag="PLS90_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPK03249.1"
                     /translation="MLKKMLMIGGLVVVVAGAGVGGLLVGRGQASGTAEHATAEDEEI
                     VADATGGHGEEAGDKAGTEEGHGAAKGGHGAEASATGGQEPVELGYEMREITTNLNDP
                     TGRQFVRIRIQLEASTPEARAQIEKNQAPLRDAALFLLSGKSPEDIQLPAGKERLKRE
                     LLARFNGLLEPNAVREVYILDSIVQRI"
     gene            5835..5996
                     /locus_tag="PLS90_12425"
     CDS             5835..5996
                     /locus_tag="PLS90_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03250.1"
                     /translation="MNRLRPPRTRRRAAPCRAQRLQTPFAPRLQWLARRQDSLHPPPQ
                     PFQPDRLVM"
     gene            complement(6247..>7552)
                     /locus_tag="PLS90_12430"
     CDS             complement(6247..>7552)
                     /locus_tag="PLS90_12430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HPK03251.1"
                     /translation="GGAVGDRRARAQADALVRGLLRRLGVVDAFLARDNLFRFNDTPD
                     PIKWVLRLAAYEWMYQAGAPDYAIGQQAVELGRLAGGPGAARFINAMMRRLMPRLPRG
                     PAALAADPYLAQMPETARLSVPPEVLREFREAYGAAEGEAVLAALAERPAPVWLRANL
                     LKSTPEEVVETLRGEGVEAAADEGLPWALRWLGGEKLPWETDAFARGLFTIQDLGAMT
                     ATLATGAQTGERVLDACAAPGGKTGQLWEMIGGQGRLVALEVDAGRRAVLAATLARLY
                     GAGCGIEVVEFNEVAHFAVQPQYNQCFDLVVVDAPCQGLGLLRRHPEIRWDGRLGRRE
                     PVLALQRGLLAAAARCVRPGGRLVWITCSPTQAENEAMLGEFRRGHEGWVLEDPLVGL
                     PPSCRGRIQVSGEGVARTRPDRADMDGFAWGCLRAPDRQYFE"
BASE COUNT         1374 a         2888 c         1972 g         1318 t
ORIGIN      
        1 ccaatcctat ttgcattttt ccccttctga attatttcgc atttcgcatt ctgtatttcg
       61 ataatttcag ctaaatcctc tccgcttata ccgccgccgc gccgagtcct caatccgttc
      121 gtacccgccc caccttgctc acgctcggcg cgggcagccc cagcttccgg ttccgcttca
      181 gcaggaacac aaacgccaga atctccgcca cggccttata gaattccgac ggaatcgtct
      241 gcccgagctt caccttccgg tacagcgcgc gcgccagcgg ccgattctca ataatcggaa
      301 tattatgccg ccgcgcgatc tcccggattc gatgcgccat cagttccgtt cccttcgccg
      361 ccacgtacgg cgcggcgtgc ttctcccggt catacgccag cgccaccgcg tagtgcgtcg
      421 ggttggtgat gatcaccgcc gccttcggca cctccgccat cattcgccgg cgcgcccgct
      481 gcatcgcaat ctgccgctgc cgctgcttga tcatcgggtt cccctcgcgc tccttcatct
      541 catccttcag ttcctggtgc gtcagcagca gcttcttgtt gaactcccac cactggtaac
      601 cgtagtcgaa taccgccagc acccccatca ggatcgccac gcggacgccg atcatcaggc
      661 tgatgtcgag catcgtctga agctggtttg tgagatcctg gcccgccagc agcttgagtt
      721 tcggcactga cttgagcagc gcgctccacg ccagcaggct gaacaggccg atcttgacgc
      781 acgccttcag cccctcgatg tactggcgcg cgctgaaaat ccgtttgaat ccgttgaccg
      841 gattgagccg gccccagtcc ggctgcagct tgttccgcgc gatgttgaac cccgtctggc
      901 agaaattcac cagcggcgac aacaccagga tcgtgcagaa cagcggcgcg accaccgccc
      961 ccgccttgat gaatggaacc agcagaaatt cgcccaccgt gtcgcggttg atcgcgccca
     1021 gatgccggtc gaaccgcagc aaatcctcca tgatccgccc gatcccctcg aaccacgcca
     1081 gccgcgcgat cccaaaaaac gtcagaaaca gcgcgaacac aaggaaggtc cccagctcgg
     1141 tgctccgggg aatttcacct ttctcccggc tctcctcgag cttcttttgg gacctgtatt
     1201 cggttttttc ctgatcgtgg tccgccatcg gtcacgcttt ctgcttgaaa tcagtccgcc
     1261 gccccgcgcc gcgtcatccg cgcatcaatc ccatcagctc gccgatcatc ccctccatgt
     1321 cgccgaacat ccccagccag agccccaccg acacgctcag catgatcatc atcatcgaca
     1381 cgccggcgat gatccgcagg ggaaagctct ccatgaatac gttgatctgc ggcaccgccc
     1441 gcgccaccat ccccagcgcc acgttaatgc agaaaatgat aatgatcacc ggcatcgcga
     1501 actgcaccga cagcagaaac agcacgtgcc ccagacgcat cacaaaatcg acgacgcccg
     1561 cgttcagcac cgcgcccccc ggcggcagca cctcaaaact ccgcgcgatc gcgccgatga
     1621 acacgtggtg cagttccgcc gccagaaaca ccagaatcgc cagcacatac tgcaattcgg
     1681 ccacgactga aatctgctgc gcccccgtcg gatcgatcgc gttgaccagt ccgaatccca
     1741 tctgctcgcc ggcaatctgc cccgagaact gaaccacccc gaacagtacc cgcccgatga
     1801 tccccacgcc gaacgccagc agcgcctcca tcagcacaag ctggacgaat tcccatggag
     1861 tgaccggaaa cgccgcgacc gccaccggcg ccaccggcgt cagcacgatc gccgtcgcca
     1921 gccccagcag cagccgcacc cgcaccggaa caaaacgctc gccgaacagc ggaaaaaaca
     1981 gaaacaccag ccccacccgc agaaagatca gcatcccgac gtagatgggg gccagcggaa
     2041 tcgaaatcat cggatcagat ccgggatcag cacgaacaga tcatgcgtat aattgagcac
     2101 cgtgttcatc atccaggaga agcacacgat gatcgcgagg atgacggcca cgattttcgg
     2161 cacgaacacc agcgtctgtt cctgaatcga cgtgatcgac tggaaaatgc tgatgctgac
     2221 gccgacgacc agcgtcgtcg ccatcaccgg caacgacagc atcagcgcga tctgaaacgc
     2281 ttcctgcagc aggtcgagga cgacttgcgg gtccatgcgc ggccgccttc ttattagaaa
     2341 taacttcgca ccagcgagcc gatgatcagg ttccaaccat cgaccagcac gaagaggatg
     2401 atcttgaacg gcagcgacac gatcaccggc ggcagcatca tcatccccat cgagagcagc
     2461 accgagctca ccaccatgtc gatgatcaga aacggaatat aaatgataaa tccaatgaga
     2521 aaggcggtct gcagttcact cacgatgaac gaggggatca gcacataggt cggcacatca
     2581 gccttggttt gcggccgctc gatgcgcgcc atccggatga acagcgccag gtcgtttttc
     2641 cgggtgtttt cgaacagaaa ctcccgcgtc ggcgtctccg cccggtcgat catctccgcc
     2701 agcgcgattt cctcattgaa atacggccgg atcgccccct catacaccga tgtccacgtc
     2761 ggcgccatca cgaagaacgt caaaaacagc gacagcccga tcaacacctg cgtcggcgga
     2821 acctcctgcg tcgaaagcgc ccgccgcaca aatcccagca ccacgacgat tcgcgtgaag
     2881 gacgtcagca tgagcagaat cgccggcgcc agcgtcagca cggtcagcag cgccagaatc
     2941 tgcagcccca tcgcgacggc cccgttgccc gactcggccg ccgcgccccc cgcgccgttc
     3001 aaactcagcg tgagcgacgg cccggcgatc gacggcagcg cgctcagatt cattccgggc
     3061 ggcgtcgcca tgcccccggc ggcgcccgcg cccgccggct gcgccgccag ccccggcgcc
     3121 agcacaagca acggcaacaa cacaagccag cgcagtctca tttgaaaacc tccagcgacg
     3181 gccggtcgcg ttccagcgac gtcgtccccc cggcgtgtgc cgccgcctcc tccaccccgg
     3241 cgcgttcggc ggccgccgac cacgcgagcg cttcgtcctc gaattccgtc agcacctgga
     3301 tcgccgccgg cgtcgcgccg agcagcagcg tcttgtcccg cgcgcgtacc agcagcagac
     3361 tccgccgttg ccccagcgtg cgggtcgcca ggatctcgat ctgacccggc cgcatgcccg
     3421 gcatcgaccg cggcaggaac cgccgcgccg cccacagcac cccgccgatc agccccagca
     3481 ccacccccat cgcgagtgtc atctgcacca tcagccgccc cagcgacggc ggatcggcca
     3541 ccgccgtctc gcccggtttt tccggcccgc cgtagtccag tgcggcaatc tcctggtccg
     3601 tcacggccca gccgcgcgcg gccccgccgg ccagcacgag caagccgaac aggaccgcca
     3661 taaccagccg gtgttttgcg atgcggataa aaagcacgag ttttccctgt cgacacgccg
     3721 cgccgttagc ggcgtatccg ttgatttttt tgtctcttcc ctgaaacgcg tctcaggcca
     3781 gcgactgcac ccgttccttg gggctcacga tatccgtcag ccggatgccg aaccggtcgt
     3841 tcaccacgac cacttcgccc cgcgccacca gcttctcgtt gaccagcact tcgaagggct
     3901 ccccgagcag cttgttcagc tcgatcaccg atcccttgcc cagttgcagc agttcctgga
     3961 tcagcagccg cgtccggccg acctccacct ttacctgcaa cggaatgtcc aggatgtagt
     4021 ccaggtccag tgccggcggt atctgcccgt tcttgggcgg attgagctcg cccggctgaa
     4081 tcgtctgcgc cgccgccgcc gcgcccgcgc ccgccgcatt ctcgttcggg gtctgcatct
     4141 gctcgctcat gtcgctttcc tttgtttgag tcctctggcc tacgcctcga ccgttcccgt
     4201 gatctgcagg gcgcgggtgc tcttgtgcgc cccgatcacc ccccagaatt tccgctcgcc
     4261 ttccaccagg atctccaccg gatgttcgta atcctcattc aactggatga tgtcgcccgc
     4321 cttcatctgc agcagctcgc ggcccgtgat cgtcgccgtc cccagcatcg cccgcagttc
     4381 caccgtcgaa tcaagcagca ggtcgcgcat ccgctgcgcc gccgcgttgt ccatgtccag
     4441 cggatcgccg ataaacgtcc ccttcaggat gtccttgatc ggctcgatcg tcgcatatgg
     4501 aatgcaaatc gacagcgacg aacgcgtccc ctcgatcacc acatcgcaat cgaccaccgt
     4561 caccggatcg ttcggctgcg cgatgttcac gaacagaatg ttcgtctcgc tgctgcccac
     4621 cgtcacggtc agcggtcgca ccggcgccca cgattccgtg tacgcccggc agatgtcctc
     4681 ggccatcttc acgatcactc gcgattcgat cagcgtgaag tcccggtcct cgcgcacatc
     4741 gaatggtttg ccgtccccgc cgaacagcag atccaccagc gtcatgatca gccgcgccgg
     4801 cagcaccagc agccccgagc ccttcagcgg ctccagcttc agcgtgtgca ggcttgacgg
     4861 cttctccagg cagcggctga aatcgccgaa attgcacacc gtctggctgc agacctggat
     4921 ctcgaccgcc cgccgcagaa tcgtcgacag gctcagccgc agcagccgcg cgaaccgatc
     4981 gtggatcgtc tccagcgtcg gcagccggcc gcgcagatag cggtcctgat ccgaaaaatc
     5041 gaacgcctgc accgcttcgg gttcctgatc ctccccgtcg ttgcgtgacg tgtcgatctg
     5101 gccgcccgat atcccaccga gcagcgcatc aatctcttcc tgggaaagca cgttggccat
     5161 cggtcccgtc tcttccgcgc ttcagatccg ttgcacgatc gaatccagaa tatacacttc
     5221 gcgcaccgcg ttgggctcca gcagaccgtt gaaccgcgcc agcagttcgc gcttgagccg
     5281 ttccttcccg gccggaagct ggatgtcctc gggcgacttg cccgacagca ggaacagcgc
     5341 cgcgtcgcgc agcggcgcct ggttcttctc gatctgcgcg cgcgcctcgg gcgtcgacgc
     5401 ctccagttgg atccggatgc gcacaaactg ccgccccgtc gggtcgttca aattcgtcgt
     5461 gatctcgcgc atctcgtagc ccagctccac cggctcctgg ccgcccgtcg cgcttgcctc
     5521 ggcgccgtgg ccgcctttgg ccgcaccatg cccttcttcc gtccccgcct tgtcgccggc
     5581 ttcctcgccg tggccgccgg tggcatccgc cacgatttcc tcatcttccg ccgtggcgtg
     5641 ctcggccgtg ccgctcgcct ggccgcggcc caccagcagg ccgcccaccc cggcgcccgc
     5701 caccacgacc accagaccgc caatcatcag catcttcttg agcatcttcg ccaatccttg
     5761 ctcccgtgaa ctccgtcgtg gccgctcccg ttggccgcga ctccttgttc ttatcgttct
     5821 tccgcgcggg aaccgtgaat cgcttgcgac cgccgcggac gcgccgccgc gccgctccat
     5881 gccgcgcgca acgcctccag acgccgttcg ccccgcggct gcagtggctg gcgcgacgcc
     5941 aggattcgct ccatcccccg ccgcagccct tccagccgga tcgcctggtg atgtgattgc
     6001 atcggtatga ttcgcctcga cttgatcttc ggacccgcgg cgccccgcag ctccggcgca
     6061 cgccctttca actcccgtgc ctgcatcggt tggcgacgct cagacttccc ccaatggggt
     6121 tccactgagg acctaaactc aattttttca gtgttggatg aaaatccgtg gcggtcgact
     6181 tcaggctcaa gtcggaagcg acgatcgtca aaagattgac caacgacgcc gattatcccg
     6241 tcagactcat tcaaaatatt gacgatcggg cgcccgcaga cacccccagg caaacccatc
     6301 catatccgcc cggtccggcc gagtccgcgc caccccttcg cccgaaacct gaatccgccc
     6361 ccggcacgac ggcggcagcc caaccagcgg atcctccaat acccaccctt cgtgcccccg
     6421 ccggaattct cccagcatcg cttcattctc cgcctgcgtc gggctgcacg tgatccatac
     6481 cagccggccg ccggggcgga cgcaccgcgc cgccgccgcc agcaggccgc gctgcaacgc
     6541 cagcaccggc tcccggcggc cgagccgccc atcccagcgg atttcgggat gccgtcgcag
     6601 cagccccagc ccttggcacg gcgcatcgac cacgacgaga tcaaagcact gattatattg
     6661 gggttgtact gcgaagtgcg caacttcgtt aaattcgacc acctcgatcc cgcaccccgc
     6721 gccatacagc cgtgccagcg tcgccgccag caccgcccgc cgccccgcgt ccacctccag
     6781 cgccaccagc ctcccctgcc caccgatcat ctcccagagc tggccggtct tccctcccgg
     6841 cgccgcgcaa gcgtccagca cccgctcccc cgtttgcgcg ccggtcgcca gcgtggccgt
     6901 catcgccccc aagtcctgta ttgtgaacag tccgcgcgca aaagcatcgg tttcccacgg
     6961 cagcttctct ccccccagcc atcgcagcgc ccagggcagg ccctcatcgg ccgcggcctc
     7021 cacaccctcg ccccggagcg tctccaccac ctcttccggc gttgatttta aaaggtttgc
     7081 cctcagccag accggtgccg gccgttccgc cagcgccgcc agcacggctt ccccctccgc
     7141 cgctccgtag gcctcgcgaa actcccgcag cacttccggc ggaaccgaaa gtcgagccgt
     7201 ttcaggcatc tgcgccagat acggatccgc cgccagcgcc gccggtccgc gcggcagcct
     7261 cggcatcaac cgccgcatca tcgcattgat aaatcgcgcc gcccccggcc cgcccgccag
     7321 ccgacccagt tcgaccgcct gctgcccgat agcgtagtcg ggcgcgcccg cctgatacat
     7381 ccactcatag gccgccagcc gcagcaccca tttgatcggg tccggtgtat cgttgaaacg
     7441 aaacaggtta tcgcgcgcaa gaaaagcatc cacgaccccg agccggcgca gcaacccgcg
     7501 caccagcgca tcggcctgcg cgcgcgcccg ccggtccccg acggcccccc cg
//
LOCUS       DAOPCO010000194         7487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8908_length_7487_cov_5.071044, whole genome shotgun sequence.
ACCESSION   DAOPCO010000194 DAOPCO010000000
VERSION     DAOPCO010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_7487_cov_5.071044"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="PLS90_12435"
     CDS             <1..1990
                     /locus_tag="PLS90_12435"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPK03252.1"
                     /translation="PWGEEAFAAARARHCPIFLSIGYAACHWCHVMERESFENEQIAA
                     YLNEHFVSIKVDREERPDLDDIYMQAVQQMTGGGGWPMTVFLTTDGKPFFGDTYFPPT
                     ARMGRIGFLDLLKQIRRAWDEEPARVREAANQLTDLLGPDVLAADAQMLTREPIERAV
                     ESLQRRFDAGRGGFGGAPKFPPSMALDLLLREARRRDDPSLTQMVELTLRRMARGGLY
                     DQLGGGFHRYATDADWLVPHFEKMLYDQALLVPVYFDAWRLTGDPFHERIGRETLDYV
                     IADLTHPDGGFYSSRDADSEGVEGKYYLWKPEEVLAELGDEQGRLFNAYYGVVEGGNW
                     HEGGGASILHVPVELEDFARRRGHEPHVLRGELDALRRRLLTRRARRVAPGLDDKIIT
                     SWNGLMIRAFAQGARATGDARYAAAAARAADFLLTNLRTPDGRLLRTWRNGQARIPAF
                     LDDYANLALGLVELYQATWDLCWLTAADELTARILTDFHDERNPGFFTTDGRDASVIV
                     RAKEFYDGATLSGNSAAVHALLRVGLLLDRPAASEAAVGALRGAMPVMTQMPPAFHFM
                     LCALDFHLAPPREVAILGPAGDDATRALLEPLGRRYLPHAIAAWADSPDAPAARAVAL
                     LRDKTLLDHRPTAYVCRGYVCRQPVHDPAAMLEEINRD"
     gene            complement(2202..2612)
                     /locus_tag="PLS90_12440"
     CDS             complement(2202..2612)
                     /locus_tag="PLS90_12440"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK03253.1"
                     /translation="MIKIDIVKRVASELNLKDREALAIVDTIIDSIKEAITEHGRMEI
                     RNFGVFQVKQRKPRTGRNPRNKKEYPILPRKVVTFKLGKELKNVAVQDDGQAGGEPVG
                     AAPKPAGASKPAAASPHAAPTGFEQTSFLPEDQE"
     gene            2979..5195
                     /locus_tag="PLS90_12445"
     CDS             2979..5195
                     /locus_tag="PLS90_12445"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPK03254.1"
                     /translation="MTPLDPPAPASPSDPNPLIEHLTGALGRAESPADLLIAATRCAA
                     QVLGAAVHNSSLFFIDAKAPITCGFLLNRHTVGADRLYRPYRLETELGERIKNASSPF
                     LSTSLALPLLVDVQASLEPDRAHNFLLVPVRDAKGPTGLWIIPLKTSLAIDQAAAAAL
                     MKLGEEMAGALRRVYRQTRDRHRMAQLEDLHEHLMPLMVATDRGLLINRLLQVLSRHF
                     AFSRVFLSLVNQESQSLRCEMHSGFETLFIPATIPLSQSENLFVGLLNQGRVALFESE
                     LGNVPPELSAFGEDPDAERIVVIPLRIDRKPLGFIYADQRRHDGHPLFPSVIDVMAQL
                     ASAALENLDLRRRAEERAETDPLTGLHNRYFMDKVLNLEIPRIRRYNHPLSVLMIDLL
                     DFKRTNDTYGHQFGDYVLRETAQLIQANVRRPDIVIRYGGDEFVVLMVNTTYEQATLV
                     RRRIEEAFIERNRLQSDESRMINISIGLKSADADCVSQLFHQADMAMYEEKARQKRRK
                     LIEALVEGSLERIEAADRVVGSLWNMLQRKAPYYPDHARRVTHLALRIARLISLGAGE
                     REILALAALLHDVGKVSIPTPILQKDTPLTHGERLAMRHHPQLGEEFFEGIEHLEPIR
                     PLIRTHHERYDGRLDGDYPAYPDGLVGQIIPLGGRIIRLAESIDGIIFGRSYRAGLGE
                     DRARAIIEVEAGRSFDPDLARSLLAQSNWTAGLGDPAAILALLNAPDPAGDCQPLA"
     assembly_gap    5313..5412
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5528..6322
                     /locus_tag="PLS90_12450"
     CDS             5528..6322
                     /locus_tag="PLS90_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HPK03255.1"
                     /translation="MDIGSIAGLILSFGLILLGNKLEGGHVNSLIQATAALIVLGGTA
                     GACILQFPMKVTINSLKGLIGVFLPRKSDPQSTIALIVDFAKRARREGILALEKEIPN
                     LTDPFFAKALRMAVDGLEPKQLVETMETELASFEEEQEGPIKFFEAAGGYAPTVGILG
                     AVLGLIHVMQNLEDPSKLGGGIATAFVATIYGVGAANLIFLPFSNKLKFNSKELVKSR
                     EIILKGVLLIQEGINHSIIEEQLKSYLDEKKRARYESEQGGRAKSD"
     gene            6353..7333
                     /locus_tag="PLS90_12455"
     CDS             6353..7333
                     /locus_tag="PLS90_12455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPK03256.1"
                     /translation="MARKKKHEEHVNHERWLVSYADFITLLFATFTALYAISNADKEK
                     FEKLAMSLRGAFNETARTEDTMLEVPKVVKTGGQNPDGALLINIISDGSAGARADADE
                     PPPGQDEDPPEEDILDGISLFRPAEAGETGDPRNAPGGPQNPATIATPTPTPEPTPAG
                     AETLPGEPEGKGTKALARELRQLLQDAGLEQFEVREEARGTVISLGEAAFFRSGGIDV
                     LPQSRATLDRIINLLRERSYEIRVEGHTDDTPVSAGRSYRNNTELSALRASRVVDYMV
                     REFGFPPDKVNPAGYGEYRPIADNATEQGRQKNRRVDIVILSDLEASKEP"
BASE COUNT         1250 a         2865 c         2061 g         1211 t
ORIGIN      
        1 tccgtggggc gaggaggcct tcgcggccgc acgcgcgcgc cactgcccga tcttcctctc
       61 catcggctac gccgcctgcc actggtgcca cgtgatggag cgcgaatcgt tcgagaacga
      121 gcagatcgcc gcctatctca acgagcactt cgtcagcatc aaggtcgacc gcgaggagcg
      181 ccccgatctg gacgacatct acatgcaggc cgtccagcag atgaccggcg gcggcggctg
      241 gccgatgacc gtcttcctga ccaccgacgg caagccattc ttcggcgaca cctatttccc
      301 cccgaccgcg cgcatggggc ggatcgggtt cctcgacctg ctcaagcaga tccgccgcgc
      361 ctgggatgag gaaccggccc gcgtgcggga ggccgcgaac cagctgaccg acctgctcgg
      421 ccccgacgtg ctggccgccg acgcccaaat gctgacgcgc gagcccatcg aacgcgccgt
      481 ggaaagcctc cagcggcggt ttgatgccgg gcgcggcggc ttcggcggcg cccccaaatt
      541 ccccccctcg atggccctcg atctcctcct gcgcgaggcc cgccgccgcg acgacccctc
      601 cttgacgcaa atggtcgagc tgaccctgcg ccgcatggcg cgcggcggcc tctacgatca
      661 actcggcggc ggcttccatc gctacgccac cgatgccgac tggctcgtcc cccatttcga
      721 aaagatgctc tacgatcagg cactcctcgt ccccgtctat ttcgacgcct ggcgtctcac
      781 cggcgatccc ttccatgagc ggatcggccg cgaaaccctc gactacgtga tcgccgatct
      841 gacccacccc gacggcgggt tctactcctc gcgcgacgcc gacagcgagg gggtcgaagg
      901 gaaatactat ctctggaaac ccgaggaggt gctcgccgaa ctgggcgacg agcagggccg
      961 cctgttcaat gcctattacg gcgtcgtcga aggcggcaac tggcacgagg gcggcggcgc
     1021 cagcatcctg cacgttccgg tcgagctgga ggatttcgcc cgccgccgcg gccatgagcc
     1081 ccacgtgctg cgcggcgagc tggacgccct gcgccggcgc ctgctgaccc gccgcgcgcg
     1141 gcgggtcgcc cccggcctcg acgacaagat catcacctcc tggaacggct tgatgatccg
     1201 cgcgttcgcc cagggggcgc gggccaccgg cgacgcccgc tacgccgccg ccgccgctcg
     1261 cgccgccgac ttcctcctca ccaacctgcg cacccccgac ggccgcctgc tgcggacctg
     1321 gcgcaacggc caggcgcgca tccccgcctt cctggacgac tacgccaacc tcgccctcgg
     1381 cctcgttgaa ctctatcagg ccacgtggga cctctgctgg ctgacggcgg ccgacgaact
     1441 gaccgcccgc atcctgaccg acttccacga cgagcggaat ccgggtttct tcacgaccga
     1501 cggacgcgac gccagcgtca tcgtccgcgc caaggagttc tacgacggcg ccaccctctc
     1561 gggcaattcc gccgccgtcc acgccctgct ccgcgtcggg ctgctgctgg atcgccccgc
     1621 cgcgagcgag gcggccgttg gcgccctgcg cggcgcgatg cccgtcatga cgcagatgcc
     1681 ccccgccttc cacttcatgc tctgcgcgct cgatttccac ctggcgcccc cgcgcgaagt
     1741 agcgattctc ggacccgccg gcgacgacgc cacccgggcg ctgctcgagc cgctgggccg
     1801 acgctacctg ccccacgcga tcgccgcgtg ggccgattca cccgacgccc ccgccgcccg
     1861 cgccgtcgcc ctcctgcgcg acaaaacgct gctcgatcat cggccgacgg cctatgtctg
     1921 ccggggttac gtctgccgcc aaccggtcca cgacccggcg gcgatgctgg aggaaatcaa
     1981 ccgggattaa ctgttgggca acgcgaagga aggacgttca caactccagc acggccattt
     2041 cgccgcaaat cggctcgccg ctctcgtccc tcgtcctcgg ctctcgaaca tccaagcgag
     2101 cgcgccagcg cagaactaga aaggaaggac agattcctcc cgtcctttat gtcccttttg
     2161 tcccttatgt cccttcgcct gaaaaatcca aaaaaaccgg cctactcctg atcctcaggc
     2221 aggaacgagg tctgctcaaa tccggtcggc gcggcgtgcg gcgacgccgc cgccggcttg
     2281 gatgcccccg ccggcttcgg cgcggcccca accggttccc cccccgcctg cccgtcatct
     2341 tgcaccgcca cgttcttcag ctccttcccg agcttgaacg tcaccacttt gcgcggcaga
     2401 atggggtatt cttttttgtt ccgcggattg cggccggtgc ggggtttgcg ctgcttgacc
     2461 tggaacaccc cgaaattgcg gatttccatc cgcccatgct ccgtgatcgc ttccttgatt
     2521 gagtcgataa tggtgtcgac aatggccagc gcctcgcgat ccttcagatt cagttcgctc
     2581 gctacgcgtt tgacaatgtc gatcttgatc atggaagctc tcccgccggc gcaagggcgc
     2641 ccgtgtgttg cgcgcgaccg cttggcagcc gcctgccccc ttgccgagcg tccttcacgc
     2701 cgcgtccctg ctcacgtatc ttgtatttct ataggcgttt agggcagcgc ctggcaaggt
     2761 ttttttctca ggtgaaccat gcccccgttc gataagacat ttacctgtgt cctctttttt
     2821 ggacaccgga tgccgcgtgg attgcccccg ccgacccccg gcggggcctt ccccccggct
     2881 ttccgcggat ggagcgtgca aggggtatgc cgactgcctc gccacacgaa ccggtcgctt
     2941 cgcgcccagg accgccccct ccggcgactc cctgtcccat gacgcccctc gatcctcccg
     3001 ctcccgcttc cccctccgat ccgaatcccc tgatcgagca cctgaccggt gccctcgggc
     3061 gcgccgaatc gcccgccgac ctgctcatcg ccgccacccg ctgcgccgcg caggtcctcg
     3121 gcgccgccgt ccacaacagc tcgctcttct tcatcgacgc caaggccccg atcacctgcg
     3181 gcttcctgct caaccgccac accgtcggcg ccgaccggct ttaccggccc tatcgccttg
     3241 agaccgaact gggcgagcgc atcaagaacg cgtcctcccc gtttctcagc acctcgctgg
     3301 ccctcccgct gctggtcgac gtccaggcct cgctcgaacc cgaccgcgcg cacaactttc
     3361 tgctcgtccc cgtccgcgat gccaaaggcc ccaccgggct ctggatcatc ccgctcaaga
     3421 cctccctcgc catcgaccag gcggcggccg cggccctgat gaaactcggc gaggagatgg
     3481 ccggcgccct ccggcgcgtc taccgccaga cccgcgatcg ccatcgcatg gctcaacttg
     3541 aagacctcca cgagcacctg atgccgctga tggtcgccac cgaccgcggc ctgctcatca
     3601 accgcctgct ccaggtcctg agtcgccact tcgccttctc gcgcgtcttc ctctcgctcg
     3661 tcaatcagga aagtcagtcg ctgcggtgcg agatgcacag cggtttcgaa acgctcttca
     3721 tcccggccac catcccgctc agccaatccg agaacctctt cgtcgggctg ctcaatcagg
     3781 gccgggtcgc cctgttcgag tccgaactgg gcaacgtccc gcccgagctg agcgcgttcg
     3841 gcgaggatcc cgacgccgag cggatcgtcg tcatcccgct gcggatcgac cgcaagccgc
     3901 tggggttcat ctacgccgat cagcgccgcc acgatggcca ccccctcttc ccctcggtca
     3961 tcgacgtcat ggcccagctc gcctccgccg cgctggaaaa tctcgacttg cgccgccgcg
     4021 ccgaggagcg cgccgaaacc gacccgttga ccggcctcca caaccgctac ttcatggaca
     4081 aggtcctgaa cctcgaaatc ccccgcatcc gccgctacaa ccacccgctc agcgtcctga
     4141 tgatcgacct gctcgatttc aagcgcacca acgacaccta cggtcaccag ttcggcgatt
     4201 acgtcctgcg cgaaaccgct cagctcatcc aggccaacgt gcgccggccc gacatcgtga
     4261 tccgctacgg cggcgacgag ttcgtcgtcc tgatggtcaa caccacctac gagcaggcaa
     4321 cgctcgtccg ccgccgcatc gaggaggcgt tcatcgagcg caatcgcctc cagtccgacg
     4381 aatcgcggat gatcaacatc agcatcggcc tcaaatcggc cgacgccgac tgcgtctcgc
     4441 agctctttca ccaggccgac atggcgatgt acgaggaaaa ggcccgccag aaacgccgca
     4501 agctgatcga ggcgctcgtc gaaggatcgc tcgaacggat cgaagcggcc gatcgcgtcg
     4561 tcggcagcct ctggaacatg ctccagcgca aggcccccta ttatcccgat catgcccgcc
     4621 gcgtcaccca cctggcgctg cgcatcgccc gcctgatctc cctcggcgcg ggcgaacgcg
     4681 agatcctggc gctggccgcc ctgctccacg atgtcggcaa ggtctcgatc cccaccccca
     4741 tcctccagaa ggataccccg ctgacccacg gcgagcggct cgcgatgcgc catcaccccc
     4801 aactgggcga agaattcttc gagggcatcg agcacctgga gccgatccgg cccctcatcc
     4861 ggacccatca cgaacgctac gacggccggc tcgacggcga ctatcccgcc tatcccgacg
     4921 gcctcgtcgg ccagatcatc ccgctgggcg gccggatcat ccgcctcgcc gagagcatcg
     4981 acgggattat tttcggccgc tcctaccgcg ccggcctggg cgaagatcgc gcgcgcgcca
     5041 tcatcgaagt cgaagccggc cgttccttcg atcccgactt ggccagatcc ctgctggccc
     5101 agagcaactg gaccgccggc ttgggcgacc cggccgcgat cctcgccctg ctcaacgccc
     5161 ccgacccggc gggtgactgt cagcccttgg cttagctcgc cccgcgccag ccgctcaccc
     5221 ccctttttcc cgattctttc cctaaagaat gtccccgtct cggacgatgc agaggatgag
     5281 tggactcgcg ccacgcgcgc ctggacaccc aannnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nntctcggac gatgcagagg atgagtggac tcgcgccacg cgcgcctgga
     5461 cacccaatgt ccgctgtccg ctcgcgcggc cccgggcagc gtaccgcaac aggatgttga
     5521 gggtctcatg gatatcggaa gcatcgccgg tctgatcttg agcttcggct tgatcctgct
     5581 gggcaataaa ctggagggcg gccatgtcaa ttcgctgatc caggccaccg ccgcgctgat
     5641 cgtcctgggc ggcaccgccg gcgcctgcat cctccagttc cccatgaagg tcacgatcaa
     5701 ctcgctcaag ggcctgatcg gcgtcttcct cccgcgcaag tccgaccccc agtcgaccat
     5761 cgccctgatc gtcgacttcg ccaagcgcgc ccgccgcgaa ggcatcctcg ccctcgaaaa
     5821 ggaaatcccc aacctgaccg acccgttctt cgccaaggcc ctgcgcatgg ccgtcgacgg
     5881 cctggaaccc aagcaactgg tcgagacgat ggagaccgaa ctggcctcgt tcgaggagga
     5941 acaggagggg ccgatcaagt tcttcgaggc cgccggcggc tacgccccca ccgtcggcat
     6001 cctcggcgcc gtcctcggcc tgatccacgt catgcagaac ctggaggatc cctccaagct
     6061 cggcggcggc atcgccacgg ccttcgtcgc cacgatctac ggcgtcggcg ccgccaacct
     6121 gatctttctc cccttctcca ataaactgaa attcaacagc aaggaactgg tcaagtcgcg
     6181 cgagatcatc ctcaagggcg tgctgctcat ccaggagggg atcaaccaca gcatcatcga
     6241 ggagcagctc aagagctacc tggacgagaa gaagcgcgcc cgctacgaaa gcgaacaggg
     6301 cggacgcgcc aagtccgact aggctccgcc gccgcacgac tggataccac ccatggcccg
     6361 caaaaagaaa cacgaagaac acgtgaacca cgaacgctgg ctggtcagct acgccgactt
     6421 catcacgctg ctcttcgcca ccttcaccgc gctctatgcg atctccaatg ccgacaagga
     6481 gaaattcgaa aaactcgcca tgtccctgcg cggcgcgttc aacgagaccg cgcgcaccga
     6541 ggacaccatg ctcgaggtcc ccaaggtcgt caagaccggc ggccagaacc ccgacggcgc
     6601 cctcctgatc aatatcattt cggacggctc ggccggcgcc cgagcggacg ccgacgagcc
     6661 cccgcccggc caggacgagg acccccccga ggaggacatc ctcgacggca tcagcctgtt
     6721 ccgccccgcc gaagccggcg agaccggcga tccgcggaac gcgccgggag gcccccagaa
     6781 cccggccacg atcgctaccc ccacgcccac gcccgaaccc acgccggccg gcgccgaaac
     6841 cctgcccggt gaacccgagg gcaaaggcac caaggccctc gcccgcgaac tgcgccaact
     6901 gcttcaggat gccggactgg aacaattcga agtccgcgag gaggcgcgcg gcaccgtcat
     6961 cagcctcggc gaggccgcct ttttccgctc cggcggcatc gatgtcctcc cccagagccg
     7021 cgccacgctc gaccggatca tcaacctcct gcgcgagcgg tcctatgaga tccgcgtcga
     7081 gggccacacc gacgacaccc cggtcagcgc cggccgcagt tatcgcaaca acaccgaact
     7141 gtcggccctg cgcgcctcgc gcgtcgtcga ttacatggtc cgcgaattcg gcttcccccc
     7201 cgacaaggtc aatcccgcgg gctatggcga ataccgcccg atcgccgaca acgcgaccga
     7261 gcaaggccgc cagaaaaacc gccgcgtcga tatcgtcatc ctgagcgacc tggaagccag
     7321 caaggaaccc tgaccccccc tcttcatccc tcgtcctcgt catcgccccc aacggcccgc
     7381 cccacattcg gctcccggct ctcgtccctc gtcctcgact ctcgaaaacc gccctccgcc
     7441 ccctcctcgc tttcgttttt tcttcgcttt ccctcttgcg cccgctc
//
LOCUS       DAOPCO010000195         7469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8939_length_7469_cov_4.135959, whole genome shotgun sequence.
ACCESSION   DAOPCO010000195 DAOPCO010000000
VERSION     DAOPCO010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7469
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8939_length_7469_cov_4.135959"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(518..4795)
                     /locus_tag="PLS90_12460"
     CDS             complement(518..4795)
                     /locus_tag="PLS90_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HPK03257.1"
                     /translation="MRWIRGSRLYHVIMLALLISVEGSAADGSPDAPTTISKSGGLTP
                     EQEALWDMTASRATPLDVEILSSHVVNNCQVQDILFTSEVGANGANRIYCGIAWPLNG
                     QGPFPPLLDLHGGGGKGSADRALSFAQQFNVLAISLDWGGKLPDDPDPLRTVWRSPPI
                     GGQETVGLNSAQHEVIVACRRALDYAAEQPDVSMAKVVVYGGSWGAYYAMLLSGIDPR
                     VTDTICIAGAGGWRDSHSLIAEGVGRLPEAQRETWYRTLDPIAYAQNTQSDVSQIAYT
                     NDSYFWLGGLQENFKALAGPKRIVIAPNNDHGIGTQPEPIATWPFPVSILKHHLEGDP
                     FPEVTSGSLQEGPPGEFSWKTTGPAVQTAALYFSPGHGNWRSRYWVEVPAHFDGTAWR
                     ASLPGHLHNVAGSYYVTVAASQNRVACTTVLSREGKDPQLEGVALWPGDSLWNTAAGR
                     LAWRTSIYHTGTTLQPAGVAGFRIVPGADAEFEVLTDSALLLGPRAQEYEGIKLTVDG
                     EGLAGTLNIRLEDDDPTRYTPAVVQTKVDYGAGQSEIVVPWSSFPAGASDQPWPFESI
                     VLAGARPGSKPITIKSVELIPLKQYPYYPILGASQGFGYINKFTDEPSPVEQGRIMVN
                     IGADAYKFMVGYNVGWASYRDANLYRDLPDLLPSGGDKTIVRLIQNEPAYRAVFDLPL
                     KFYVMWTYATDYWWFRSSIPDSDMQKEYSQLYDFTRYLLQTYQGTGKVFLMGQWESDH
                     VIMEGRDANNPALQPTAQMIDAMRRWFANRQQAIDDARNSLPEITGVEVYNYVEVNCI
                     QPVLDNPNVQRVVNVVLPYVTVDLISYSSYNATNHTEQLPQRLNAHMDFIESKANLTG
                     RWPYGKPVFIGEYGMPRPYDNSRAAQNLASLKACAAWGSPINLFWSIYSQLENGNSAL
                     VENNGTPNRDYYQLRDFNRKMHVLRNATRVWLDRNPTEAETRAFINGFTYPAGANYTL
                     LANVINASGERTSNAQYLAALCQDLNLGEQIGTAWYQGLLDDLAAGRKTRSAVLSAVL
                     DSAEFAAAVPLEDFKSYLADAVLRQEGVQFPTPEPSRSSLYQWAINQAQFNEADLPYA
                     LLSCVPDTLRAKYSLLLSQTEKQSGAFFEDFKDGAATFGPSYLIGGGEGVSVALTAGS
                     ELLEVLSTNAHTDGTTSWGIEYPKEGTIRWDTAQNRYLQAWIPWKGGGSDLLAVDFQI
                     DGASLWQGDWWKNRIDNAEQWYHSQPTLVDVNTLANLTPGVHQLRMRFVDFNTFYGVG
                     EDLSTLGMAIDWIRGGMPPDADVSLAQYKNSSGSATPVTCGFVGPEDEAIANNPPTLV
                     WNPPAGHGDLRYTVSLSQDPFFRGPTTVTVHDITATHYTPAAPLDLGRWYWTVIPVNS
                     DGISGQDLIKSLRSGGHWNNPQDYSFYSFVIDESSSRNSATTWPLYH"
     gene            5364..7313
                     /locus_tag="PLS90_12465"
     CDS             5364..7313
                     /locus_tag="PLS90_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK03258.1"
                     /translation="MKQVFDCSQLDWVVAGLIPDLWRFLPPRHIAERRDAEIPAVPVR
                     VPGSVQQALLDAGHLPDWNIGLNARLCEWVENRDWIFEVTLPDEWFRPGLQYRLHCLG
                     LDYCGEVVFNGHDVGAFNNAFVPHVFDLTPHLLPTENVLQIAFTPPPPRAMGQFSWTS
                     RATDWKPRFNFFWNWVCRLVQVGIWDAVYIEASDGREITHLRCVGDADPQTGKGALRI
                     HAAAAGAETRVRLTLEDGERTLREELVDPARLAGDGLLWDNLDVRLWWPNGLGDQPLY
                     TLRCELLDRAGARLDVQTRRVGFRHVAWEPCRDAPAGAEPWICVVNGRRLFLQGVNWT
                     PIRPNFADVPREEYQRRLQTYRDLHMNMLRVWGGAFLEKECFYDLCDELGLMVWQEMP
                     LSSSGRDSYPPDTEPAMTEQCAIARSYVERRQHHPALIMWCGGNELTERGPERRPIDL
                     RHPLMARFAAIFQQMDPTRRFIPTSPSGPVGFPTPDQVGKGILWNAHGPWEAHDLEEW
                     DEMFRRDDALFRAEIGDRSASPVDLIRRTRGELPEMPPTPDNPLWRRQPWNVSEQDFR
                     REVGRPPATLEEFVDWSQRRQTRTLTTVAESCKRRFPRCGGVLFWMGHDCFPCAGNNS
                     LLDFDGRPKPAALAIGEIFKRPVTG"
BASE COUNT         1281 a         2555 c         2249 g         1384 t
ORIGIN      
        1 atacagcata tcctggtggg ccgtgctctg gcccacgtag cggacgacca ccggatcagt
       61 gccggtcgcc atcagatttc ccccggggat ggtcagtgca tggccggtcg tggcccagca
      121 aagcaacagc agggctggca gcgccaaccc acgcctggac agaatcatgg caaacgctcc
      181 tggaacggga atcggaacaa ccgcaccccc ggcagcgtcc ggaggtgtct ctgcgcatgg
      241 tcccagtcag ccggagcgcc cggattttgt caaggcataa aatgttcact atgaaaattt
      301 tcatgggtgc gcagttcatg tgcgcatatc cgcccccgcc gtgaaccgcc ccccaaaccc
      361 gcttctccgg ggaggcagcc caagacgtgg cggcattcga tctttgcccg cccatggatg
      421 ggagccatgc atgcgtcgcg agcacgcgat gacccggtga cgaccggcat gcgggggccg
      481 ccggcgccgc gttccgcctt cgcctcaggc ggcgcgctca gtgatacagc ggccaggttg
      541 tcgcgctgtt ccgcgaggag gattcatcaa tcacaaatga atagaatgag tagtcctggg
      601 gattgttcca atgcccgccc gaccgcaggg acttgatcag atcctgtccg ctgattccgt
      661 cgctgttgac cggaataacc gtccagtacc accggcccag atcgagcgga gcggcgggag
      721 tgtagtgcgt tgcggtgatg tcgtgcaccg tgaccgtcgt cggtccgcgg aagaacggat
      781 cctgagacag gctgacggtg tagcgcaggt cgccatgccc ggccggtgga ttccagacga
      841 gcgtcggcgg attgttcgcg atggcctcat cctccggccc gacaaagcca caggtcacgg
      901 gcgttgcgct cccgctgctg tttttgtatt gtgcgaggct gacatccgca tccggaggca
      961 ttccgccgcg gatccagtcg atcgccatcc ccagcgtgct caaatcctcg ccgacgccgt
     1021 aaaacgtatt gaaatccacg aagcgcattc tcagctgatg cactcccggc gtcaggttgg
     1081 ccagcgtatt aacgtccacc agtgtcggct gggaatggta ccactgctcg gcattatcaa
     1141 tccggttctt ccaccagtcg ccctgccaca aggacgcccc gtcaatctgg aagtccacgg
     1201 ccagcaggtc gctgcctcct cccttccacg gaatccatgc ctgcaaatac cggttctggg
     1261 ccgtgtccca gcggatcgtc ccctccttcg ggtattcgat gccccacgag gtcgttccat
     1321 cggtgtgggc attggttgag agcacctcga gcaattcaga tcccgctgtc agtgcgacgc
     1381 tgacgccctc gcccccgccg atcaggtagg atggtccgaa agtggccgcg ccgtccttga
     1441 aatcctcgaa gaacgccccg gattgcttct ctgtctgcga aagcagcagg gaatactttg
     1501 cgcgcagggt atccggaacg cagctcagca gggcataggg caggtccgct tcattgaact
     1561 gcgcctgatt gatcgcccat tggtataagc tggaccggct tggctcggga gtggggaact
     1621 ggacgccttc ctgccgcagc acggcgtcgg ccagatagct cttgaaatcc tcaagcggca
     1681 cggcggccgc gaattcggcg gaatccagca cggctgacag gacggcgctg cgggtcttcc
     1741 gccccgccgc cagatcatcc agcaggccct ggtaccaagc cgtcccgatt tgctcgccca
     1801 ggttcaggtc ctggcacagc gcggcaaggt attgggcatt gctggtgcgc tcgccggagg
     1861 cattgatcac attggccagc agggtgtaat tcgcaccggc gggataggtg aaaccattga
     1921 tgaaggctct cgtttcggct tcggtcgggt tgcgatcgag ccacacccgc gtcgcgtttc
     1981 gcagcacatg catcttgcgg ttgaagtcgc ggagctggta gtaatcccgg ttcggcgtcc
     2041 cgttgttctc cacaagggcc gaattgccat tctccaattg ggagtaaatc gaccagaaca
     2101 gattgatcgg cgatccccac gccgcacacg ctttcagcga cgccaggttc tgcgcggccc
     2161 ggctgttgtc gtatggccgc ggcatgccgt attctccgat gaagaccggt ttgccatacg
     2221 gccagcgccc cgtcagattg gctttgctct cgatgaaatc catgtgagcg ttgagccgct
     2281 gcggcagctg ttcggtgtga ttggtggcgt tatacgagga ataggagatc aggtcgaccg
     2341 tcacgtacgg cagaaccaca ttgaccacgc gctgcacatt ggggttgtcg aggaccggct
     2401 gaatgcaatt gacctcgacg tagttgtaga cttccacccc cgtgatctcc ggcaacgagt
     2461 tgcgggcatc gtcgatcgcc tgctgccggt tcgcgaacca ccggcgcatc gcgtcgatca
     2521 tctgcgcggt cggctgcagt gccggattgt tggcatcccg accttccatg atcacatggt
     2581 cgctttccca ttgccccatc aggaagacct tgcccgtccc ctgataggtc tgcagcaggt
     2641 agcgcgtgaa atcatagagt tgcgagtatt ccttctgcat gtcgctatcc ggaattgaag
     2701 aacggaacca ccagtagtcg gtggcgtagg tccacatcac atagaatttc aacggcaagt
     2761 caaaaaccgc ccggtaggcc ggttcgttct ggatcaggcg gacgatcgtc ttgtccccgc
     2821 cactgggcaa caggtcgggc aggtcccggt agaggttggc gtcgcgatag cttgcccaac
     2881 cgacgttata gccgaccatg aacttgtagg cgtcggcgcc gatgttgacc attatccggc
     2941 cctgctcgac gggagacggc tcatcggtga acttgttgat gtatccaaac ccctggcttg
     3001 ccccgagaat cggatagtag ggatattgtt ttaacggaat caattccacc gatttgatgg
     3061 taatcggctt tgaaccgggc cgcgcgccgg ccagcacgat gctctcaaag ggccagggct
     3121 gatcgctcgc tcccgccggg aaactgctcc acggcacgac gatctcactc tgcccggctc
     3181 cgtagtcgac cttggtctgc acgacggccg gggtgtaacg cgtcgggtca tcgtcctcca
     3241 gccggatgtt cagcgtgccg gccagcccct cgccatccac ggtcagcttg atgccctcat
     3301 actcctgggc gcggggcccg agcagcagcg cgctgtcggt cagcacttca aattcggcgt
     3361 cggcgcccgg gacgatgcgg aacccggcaa cgccggccgg ctggagcgtc gtgccggtat
     3421 ggtagatcga agtccgccag gccagtcgtc ccgcggctgt gttccagagc gaatcacccg
     3481 gccagagcgc aaccccttcg agctggggat ccttgccctc gcgcgacagg acggtagtgc
     3541 aggccacgcg gttttgactc gccgcgaccg tgacatagta gcttccggcc acgttatgca
     3601 ggtggcccgg cagcgacgcc cgccaggcgg ttccatcgaa atgcgccggc acctcgaccc
     3661 aatagcgtga gcgccagttg ccatgaccgg ggctgaaata caatgcggcc gtctggaccg
     3721 ccggccccgt cgtcttccag ctgaactcgc cgggcgggcc ctcctgcagc gatcccgatg
     3781 tcacttcggg aaaggggtcc ccctccaggt ggtgtttcag gatcgatacc ggaaacggcc
     3841 acgtggcgat cggctcgggt tgcgtcccga tgccatgatc gttgtttggc gcgatcacga
     3901 ttcgtttcgg cccggccagc gccttgaagt tctcctgcag accaccgagc cagaagtacg
     3961 aatcgttggt gtaggcgatc tggctgacgt cggactgcgt gttctgggcg tacgcgatcg
     4021 gatccagggt gcggtaccag gtctcgcgtt gcgcctcggg cagccggccc accccctccg
     4081 cgatcaggga atggctgtcg cgccatccgc cggccccggc gatgcaaatg gtgtcggtca
     4141 cgcgcggatc gatcccggac agcagcatgg catagtacgc gccccacgaa ccgccgtaga
     4201 ccacgacctt ggccatcgac acgtccggct gctccgcggc gtaatcgagc gcgcggcggc
     4261 aggccacgat cacttcgtgc tgcgcgctgt tcagccccac cgtttcctgg ccgccgatgg
     4321 ggggcgaacg ccagacggtg cgcagcggat ccggatcgtc gggcagcttc ccgccccaat
     4381 cgagcgagat cgccaacacg ttgaactgtt gcgcaaagga caacgcacgg tcggccgagc
     4441 ccttgccgcc cccgccgtga aggtccagca ggggggggaa gggtccctga ccattgagcg
     4501 gccaggcgat gccgcagtag atccgattgg cgccgttcgc cccgacttca ctggtaaaca
     4561 ggatgtcttg cacctggcag ttgttgacaa catgggagga aagaatctcc acatccagtg
     4621 gggtcgcgcg actggcggtc atgtcccaca gggcttcttg ttccggcgtc aggccgccgc
     4681 tcttgcttat tgtggtcggc gcatcgggcg acccgtccgc ggcgctcccc tcaacggaga
     4741 tcagcagcgc cagcattatc acgtgataca gtcgggaccc gcgaatccac cgcatggatc
     4801 tggacctgcc tttcgtcgac atctctcggc tgtcgtctgg attcaacggg gggattacgg
     4861 atcggcctgc ttcccgcgca ctcgtccctg gtgtcccggt caagcgtgca acctctcagc
     4921 tgatcctccg ccgcattcgc tttgtctgat cgaatagttg aataattccg aatttcccac
     4981 gcccactgat tctttcccgt tccccgcgac cccgtcaagc ccaaacgctt ttgttgggtg
     5041 gagaggctgg ccgcgaaatc cttccggatc aatgcgggcg ccaaaaaaaa cttgacgagg
     5101 gaaccagcgg accggatcat tccggagtct tcggtgtgat tatgctttcg gttccatgtg
     5161 aatctctcgg gagacgatca ggcatgaact ggcttacgcg ccgcgggttt ctcaagtcgg
     5221 tcgccggctc gtcgctgtcg gcgggtttgc tcggttgcat gggggcggac gggctgtttc
     5281 ccgcaccttc cccagatcac gtgataagct ccaccgcctc ccccgcgccc cttccctcca
     5341 aaccctgcca aggaatcccc tccatgaaac aggttttcga ttgttcccaa ctggactggg
     5401 tcgtcgccgg cctcatcccc gatctctggc gcttcctgcc cccccgccac atcgccgagc
     5461 gccgcgacgc cgaaatcccc gccgtcccgg tccgcgtccc cggctcggtc cagcaggccc
     5521 tgctcgacgc cggccacctg cccgactgga acatcgggct caacgcccgg ctttgcgaat
     5581 gggtggaaaa tcgcgactgg atcttcgagg tcactctgcc cgacgaatgg ttccggcccg
     5641 gcctccaata ccgcctccac tgcctcgggc tggattactg cggcgaggtc gtgttcaacg
     5701 gccacgatgt cggcgccttc aacaacgcct tcgtcccgca cgttttcgac ctgacgccgc
     5761 acctgcttcc cacggaaaac gtcctgcaga tcgccttcac cccgcccccg cctcgcgcca
     5821 tgggccagtt cagttggacc agccgcgcca ccgactggaa gccgcgcttc aactttttct
     5881 ggaactgggt ctgccgcctg gttcaggtcg gcatctggga cgccgtctat atcgaggcct
     5941 ccgacggccg cgagatcacc cacctgcgct gcgtcggcga cgccgacccg caaaccggca
     6001 agggcgccct gcgcatccac gccgccgccg ccggcgccga gacccgcgtc cgcctcaccc
     6061 tggaggatgg cgagcggacc ctccgcgagg agctcgtcga tccggcgcgc ctcgccggcg
     6121 acggcctcct ctgggacaac ctcgacgtcc gcctctggtg gcccaacggc ctgggcgacc
     6181 agccgctcta cacgctgcgc tgtgaactgc tcgaccgcgc cggcgcccgg ctcgacgtgc
     6241 agacccgccg cgtcgggttc cgccatgtcg cctgggagcc gtgccgcgac gcgcccgccg
     6301 gcgccgaacc ctggatctgc gtcgtcaacg gccgccgcct ctttctccag ggcgtcaact
     6361 ggacgccgat ccgccccaac ttcgccgacg tgccgcgcga ggagtatcag cggcgcctcc
     6421 agacctaccg cgacctccac atgaacatgc tgcgcgtctg gggcggggcc ttcctcgaaa
     6481 aggaatgctt ctacgatctc tgcgacgaac tcggcctgat ggtctggcag gaaatgcccc
     6541 tgtcatcctc cgggcgcgac agctacccgc ccgacaccga gccggcgatg accgaacagt
     6601 gcgcgatcgc ccgctcctac gtcgagcgcc gccagcatca ccccgccctc atcatgtggt
     6661 gcggcggcaa cgaactgacc gagcgcggcc ccgaacgccg acccatcgac ctgcgtcatc
     6721 ccttgatggc ccgcttcgcg gcgattttcc agcagatgga ccccacccgc cgcttcatcc
     6781 cgacctcccc cagcggcccg gtcgggttcc ccacgccgga ccaggtcggc aagggcatcc
     6841 tctggaacgc ccacggcccc tgggaggccc atgacctcga ggagtgggac gagatgttcc
     6901 ggcgtgacga tgccctgttc cgcgccgaga tcggcgaccg cagcgccagc ccggtcgacc
     6961 tgatccggcg cacccgcggc gagctgcccg aaatgccccc cacccccgac aacccgctct
     7021 ggcggcgtca gccgtggaac gtcagcgagc aggacttccg tcgcgaggtc gggcggcccc
     7081 cggccaccct cgaggaattc gtcgactgga gccagcgccg ccagacccgc acgctgacca
     7141 ccgtcgccga gagctgcaag cggcgcttcc cccgctgcgg cggcgtcctc ttctggatgg
     7201 gccacgactg cttcccctgc gccggcaaca actcgctgct cgacttcgac ggccgcccca
     7261 aaccggccgc cctggccatc ggcgaaatct tcaagcgccc cgtcaccggc tgacccgcgt
     7321 cccgcccgtc ttcccggttt gagggatcaa atagcccacc ggatccgggg cggatcgtgc
     7381 ctcgccgcat cgcgcccctc cctgcgttgc gcgccggcga ccgcgagacc gaaacgtgat
     7441 gcagagccca aggtgagatc aatttccta
//
LOCUS       DAOPCO010000196         7457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_8959_length_7457_cov_5.823831, whole genome shotgun sequence.
ACCESSION   DAOPCO010000196 DAOPCO010000000
VERSION     DAOPCO010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8959_length_7457_cov_5.823831"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="ruvB"
                     /locus_tag="PLS90_12470"
     CDS             complement(<1..1009)
                     /gene="ruvB"
                     /locus_tag="PLS90_12470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPK03259.1"
                     /translation="MPTDPTRPLTGARLEEDLAADNTLRPASTREFIGQREILEQLIL
                     SIEAARARGEALDHVLLYGPPGLGKTTLAKIIANEMGTTIKETSGPLLERKDDLAAIL
                     TDLQPGDVLFIDEIHRLARVVEECLYPALEDFQIDILIGEGPHAKSIKLELPQFTLVG
                     ATTRAGMITAPLRTRFGMLHRLNFYEGADMERIVARSARILEVEIEPEGAAEIARRSR
                     GTPRIANRLLRRVRDWAQVRGDGRITHEAAARALKMLQVDERGLDQMDRELLRTLIEK
                     FGGGPVGLNTLAVAIGEEEDTLIDVYEPYLIQIGFLHRTPRGRVATARAFEHMGVRPP
                     AS"
     gene            complement(1096..3165)
                     /locus_tag="PLS90_12475"
     CDS             complement(1096..3165)
                     /locus_tag="PLS90_12475"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK03260.1"
                     /translation="MIEVLDINGGANGPECRVRLICHCGAPRLAIYSKVTDRIIYCAN
                     CGSITTLTRLRREWADDPAARGEWQLINVREGDECPLVNVFVPVRLVFDVGDRKGCES
                     QGVLTKLSINKAFLRLKEYEEGDPQAPPSLAKGQYLRLEGNDGPMKGRTLACIEDHVT
                     PPTPDSPVYYKLRFELLMPRRREELEKFYREACGLTTRADGTPRVQSNGRARRKVELR
                     PPDTEPELDLEEAHTPRTPESSPGEPAPPEESRLLVFGRGLNGATPNGAVQNGAAANG
                     VLPGAAGSPPEPEWPAEPEDEPPAVPMANATNDTLGDDDPAPRYAPRPAPAQTPVRPV
                     AAEPRVETTAPIAAASLEDDSIRLLILTSETHDQNISNHLKRISSYSAAIARELNWSD
                     EAAARLATASKLHDLGKIGVPDQILKKTGALTEEERTTMQQHTRLGYHILENSASEIM
                     RLGAVIALRHHERYDGTGYPDGLVGDAIPIEAQIVSIADVFDALTTARPYKPAWPTDK
                     TVQYIVDQAGKMFNPQVVDAFLKAMPEIKRNQLRFIDDFRDIWTERRNATRVPVAPVP
                     ISIEVAMPEMTFRPYRLEGIICNLSAYGVKARLNNVTGDMFGLLVNSRRYAKIYCEQP
                     LWEPLHQTFCRVAWIDYYALPDPDACLMGLEFQNPIHELVEIIQEIGTAPQPEGVEGI
                     ERIDSAG"
     gene            3406..4341
                     /locus_tag="PLS90_12480"
     CDS             3406..4341
                     /locus_tag="PLS90_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03261.1"
                     /translation="MPSTLSTARHRIVAVSDDAEVIREVEERLEVFGAEFIGLLADGG
                     LLPGLIEAAPDLVVMDSSGDSVRDLRVISAVRRRAQQCRFILLVSREQLDHMRDMTRS
                     RAIAYLGKPVLADELNALVERLLRVQRSPVNRREHSRFSLVLQTTCHVINPFNDSEGH
                     AVAALVRDVSRDGISLLVRQLMPVPAIIKLVFHIPGQNAPVQVLVRSIACTLTQIPGA
                     YRIGARFVGLLPAPLDAVIGRLNGAAPMDGDLFLGKSFRDAVEDWLKAHQEEAVTRAV
                     IGSAPLGDLVNELCADVEESPVFQGVLAQVRASEG"
     gene            complement(4344..5054)
                     /locus_tag="PLS90_12485"
     CDS             complement(4344..5054)
                     /locus_tag="PLS90_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03262.1"
                     /translation="MAKQRNPLITVDGRRWRRIPIRTHRVTREDDIVELCDRYSRELR
                     QAGDILFVSEKVVAVTQGRAIPVSEIRIGLLARLLWRCVRKVPYGVGLRSPETMQCAI
                     NECGAARILAAAAIGGLTRAFGRRGDFYRIAGMQAATIDAAGTSPLQSDCVILGPKDP
                     DDVARRLRARTGLAAAVVDVNDIGGSWVLGSDGIADPRLIEAILKDNPLGQKDEQTPF
                     GLIRAEAAEAAPPAGPVG"
     gene            complement(5079..5927)
                     /gene="dapF"
                     /locus_tag="PLS90_12490"
     CDS             complement(5079..5927)
                     /gene="dapF"
                     /locus_tag="PLS90_12490"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPK03263.1"
                     /translation="MRFHKYHALGNDYLVMDPADGIERLEPTAVRKICHRNYGVGSDG
                     ILHGPLATNKGDFGLRIWNPDGSEAEKSGNGLRIFSRFLLDSGRVQPGRPFTVDTPGG
                     RVRCEVNAGGARVTVEMGRVSFDAAQIPVSGVAGEVLGQQLTAGEMIVTYNAATIGNP
                     HCVVVRDEVSAEEARALGPQLENHPIFPNRTNVQFLQVLDRNNIRIEIWERGAGYTLA
                     SGSSSSAAAAVAHRLGLCDRDITVHMPGGNLRIQIAEDYAITMTGPVTRVAAGEIDDE
                     LFTVEI"
     gene            6158..6712
                     /locus_tag="PLS90_12495"
     CDS             6158..6712
                     /locus_tag="PLS90_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03264.1"
                     /translation="MNRNLLFATLTAGLALAGVIAAQTAAPRLERPLPKTIIRGNLHV
                     DGERTWNAQHVVIHGDLILEEGGRLEVNDSVVELVGEAAGDRVIRWRGGALLTIDSTL
                     GGTPGTDDAPSAPADLLLLDGDWGTTRTTLQHIGELRVGGAPPGGHLWGTGLTARDQV
                     GPVLLSGAGRIDLVDSDVRIEPAP"
     gene            6856..>7457
                     /locus_tag="PLS90_12500"
     CDS             6856..>7457
                     /locus_tag="PLS90_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK03265.1"
                     /translation="MATRKADAIIIGSGQAGVPLALKWDRTGKKVILFEKGALGGSCV
                     NYGCTPSKAFLAAAHRAQSVRSARELGVYADVRVEFAEVMKRVRDVRAEWNEGVGRNF
                     KNSGIELIEGVARFAGERRVEAQTAAGTETAEAPLIVIDTGSAPRIPAIPGLEGTPFL
                     TNMNFFDLREQPRCLIVLGGGYVGLELGQGMSRLGSEVHIL"
BASE COUNT         1111 a         2517 c         2549 g         1280 t
ORIGIN      
        1 gcgaggcggg ggggcggacg cccatgtgct cgaacgcccg cgccgtggcg acccgtccgc
       61 gcggcgtccg gtgcagaaat ccgatctgaa tcagatacgg ttcatacacg tcgatcagcg
      121 tgtcctcttc ctcgccaatg gcgaccgcca atgtattcag gccgacgggg ccgccgccga
      181 atttctcgat cagcgtccgc agcagctcgc ggtccatctg gtccagtccg cgctcatcga
      241 cctgaagcat cttgagcgcg cgggcggcgg cctcgtgtgt gatgcgcccg tcgccgcgca
      301 cctgcgccca gtcgcgcacg cggcgcagca ggcggttggc gatgcgcggc gtgccgcggc
      361 tgcgccgggc gatctccgcc gccccctccg gctcgatctc gacctccagg atccgcgccg
      421 agcgcgcgac gatccgttcc atgtccgcgc cctcgtagaa gttgagccgg tgcagcatgc
      481 cgaaccgcgt gcgcagcggg gcggtgatca tcccggcgcg cgtcgtcgcg ccgaccagcg
      541 tgaactgcgg cagctccagc ttgatcgact tggcgtgcgg cccctcgccg atcaggatgt
      601 cgatctggaa atcttcgagc gccgggtaga ggcattcctc gacgacgcgc gccagccggt
      661 ggatctcgtc gatgaacagc acgtcgcccg gctgcaggtc ggtcaggatc gccgccaggt
      721 cgtccttgcg ctcgagcagc ggcccggacg tttccttgat cgtcgtcccc atctcgttgg
      781 cgatgatctt ggccagcgtg gtcttgccca gaccgggcgg gccgtaaagc aggacgtgat
      841 cgagcgcctc gccgcgggcg cgggcggcct cgatcgacag aatcagttgt tcgagaatct
      901 cgcgctggcc gatgaattca cgcgtcgagg ccggccgcag cgtgttgtcc gcggccagat
      961 cttcctccag gcgtgcgccg gtcagcggtc gcgtcgggtc ggtgggcatg gggaacgtcc
     1021 ggcggcgtcg ggcacgcggc gatcatgcgg ccctcaaggg cggtcgtccc attgaaccgg
     1081 tttgcgcggg ctacgtcaac cggccgaatc gatccgttcg atcccctcca ccccctccgg
     1141 ttgcggggcg gtgccgatct cctggatgat ctcgaccagc tcgtgaatcg gattctggaa
     1201 ttccagtccc atcaggcagg cgtcggggtc cggcagcgcg tagtaatcga tccacgccac
     1261 gcggcagaac gtctggtgca gcggttccca gagcggctgt tcgcagtaga tcttggcata
     1321 gcggcgcgag ttgaccagca ggccgaacat gtcgccggtg acgttgttca gccgcgcctt
     1381 gacgccgtag gccgagaggt tgcagatgat cccctccagc cggtacgggc ggaaggtcat
     1441 ttcgggcatg gccacctcga tgctgatcgg cacgggggcc accggcacgc gcgtggcgtt
     1501 gcgccgttcg gtccaaatgt cgcggaagtc gtcgatgaac cgcaactggt tgcgtttgat
     1561 ctccggcatc gccttgagga acgcatcaac gacctgcgga ttgaacatct tgccggcctg
     1621 gtcgacgatg tattgcaccg tcttatccgt gggccaggcg ggtttatagg ggcgcgccgt
     1681 ggtcagcgcg tcgaagacgt cggcgatcga gacgatctgc gcctcgatcg ggatggcgtc
     1741 gcccacgagc ccgtcggggt aacccgttcc gtcgtagcgc tcgtgatggc gcagcgcgat
     1801 caccgcgccc agccgcatga tttccgacgc gctgttctcc agaatgtggt agcccagccg
     1861 cgtgtgctgc tgcatcgtcg tgcgctcttc ctcggtcagc gcgccggtct ttttcaggat
     1921 ctggtcgggg acgccgattt tgcccagatc gtgcagcttg gaggcggtcg ccaggcgggc
     1981 cgcggcctcg tcggaccagt tcagttcgcg ggcgatcgcc gcgctgtagg acgagatccg
     2041 tttgagatgg ttcgaaatgt tctggtcgtg ggtttccgac gtcaggatca acagccggat
     2101 cgagtcgtcc tcgagtgagg cggcggcaat cggcgcggtc gtttccacgc gcggctcggc
     2161 ggcgaccggg cggaccggtg tctgggccgg tgccggccgc ggcgcatacc tcggcgccgg
     2221 atcatcgtcg cccagtgtgt cgtttgttgc gttggccatc ggcacggccg gcggttcatc
     2281 ctccggctcg gcgggccatt ctggctccgg cggcgaaccc gccgcgccgg ggagaacgcc
     2341 gttggccgcg gcgccgttct ggactgcgcc attcggagtt gcgccgttca gcccgcgccc
     2401 gaaaaccagc aggcgcgact cctccggcgg cgccggctcg ccgggcgacg attccggcgt
     2461 ccgcggcgtg tgcgcttctt caagatccag ttccggttcg gtgtcgggcg ggcgcagttc
     2521 caccttgcgc cgggcgcgtc cgttcgactg gacgcggggc gtgccgtcgg cccgcgtcgt
     2581 caggccgcac gcctcgcggt aaaatttctc cagctcttcc cgccgccgcg gcatgagcaa
     2641 ctcaaaccgc agcttgtagt aaaccgggct gtcgggcgtc ggcggcgtca cgtgatcctc
     2701 gatgcacgcc agcgtgcgcc ccttcatcgg gccgtcgttg ccttccaagc gcaggtattg
     2761 ccccttggcg agcgacggcg gcgcctgcgg gtccccctcc tcgtattcct tcagacgcag
     2821 gaaggccttg ttgatcgaca acttggtcaa caccccctgc gattcgcagc cttttcggtc
     2881 ccccacgtcg aagacgagcc ggacgggaac gaacacattg accagcgggc attcgtcccc
     2941 ttccctgacg ttaatcaact gccattcgcc gcgggccgcc gggtcgtcgg cccactcgcg
     3001 gcgcagacgc gtcagcgtcg tgatgctgcc gcagttggcg caatagatga tgcggtccgt
     3061 gactttgcta tagatcgcca ggcgcggcgc gccgcaatgg cagatcagcc gcacacggca
     3121 ttccgggccg ttggcgccgc cgttgatgtc cagtacttcg atcaagtgga tgcacccttc
     3181 tgggacgtcg cccgcagaaa gtctcccaaa acgtctcgcg ttccctgttt cttttcgtga
     3241 gtcagggccc gcggctttag cgggagtcaa aattcgattc gccggggggc ctgtccggtt
     3301 ccggtcccgg gcctcaagcg gaggggaatc cggccgatag aacgatcatg agccctctcg
     3361 cgccgatccg gcgccgggag gagaatccgt gtttatgggg tttgcgtgcc ctcaaccctg
     3421 tccacagccc gccaccggat cgtcgccgtc agcgacgacg ccgaggtgat ccgcgaggtc
     3481 gaagagcggc tcgaggtttt cggcgccgag ttcatcgggc tgctcgccga cggcggtctg
     3541 ctccccggcc tgatcgaggc cgcgccggac ctggtcgtca tggactcatc gggcgacagc
     3601 gtgcgcgacc tgcgcgtgat cagcgccgtg cgccgccgcg cgcagcagtg ccggttcatt
     3661 ctgctcgtct cgcgcgagca gctcgatcac atgcgcgaca tgacgcgcag ccgcgccatc
     3721 gcctatctgg gcaaaccggt cctggccgat gaactcaacg ccctcgtcga gcggctgctg
     3781 cgcgtccagc ggagcccggt caatcgccgc gaacacagcc ggttttccct tgttctgcaa
     3841 accacctgcc acgtgatcaa cccgttcaac gacagcgagg ggcatgccgt ggcggcactg
     3901 gtgcgcgatg tcagccgcga cggcatctcg ctcctcgtcc gccagctcat gccggttccg
     3961 gcgatcatca agctggtctt tcacatccca ggccagaacg cgccggtgca ggtcctcgtg
     4021 cggtcgatcg cctgcacgct gacgcagatc cccggcgcct accgcatcgg cgcccggttc
     4081 gtgggcctgc tgcccgcccc gctcgacgcg gtgatcggcc ggctgaacgg cgccgcgccg
     4141 atggatggcg acctgtttct gggcaagagt ttccgcgacg ccgtggagga ctggctcaag
     4201 gcgcaccagg aggaagccgt cacgcgggcc gtgatcggca gcgcgccact gggcgacctg
     4261 gtcaatgagt tgtgcgcgga tgtggaggaa tcgcccgtct tccagggggt gctggcccag
     4321 gtgcgggcga gcgaaggctg aagtcagccg accggcccgg ccggcggggc ggcctccgcc
     4381 gcttcggcgc ggatcaggcc gaacggcgtc tgctcgtcct tctgccccag cggattgtcc
     4441 ttgaggatgg cttcgatcag gcgcgggtcg gcgatgccgt ccgaaccgag cacccatgag
     4501 ccgccgatat cgttgacgtc gacgacggcc gcagccagcc cggtccgcgc gcgcagccgg
     4561 cgcgcgacgt cgtcgggatc cttcggcccc aggatcacgc agtccgactg cagcggactg
     4621 gtgccggcgg cgtcgatcgt ggcggcctgc atgccggcga tgcggtaaaa atcgccgcgg
     4681 cggccgaagg cgcgggtcag cccgccgatc gccgccgccg ccagaatccg cgccgccccg
     4741 cattcattga tcgcgcactg catcgtttcg ggcgagcgca ggccgacgcc gtagggcacc
     4801 ttgcgcacgc agcgccacag cagccgcgcc agcagtccga tccggatttc gctgaccggg
     4861 attgcccggc cctgcgtcac tgccacgacc ttctcgctga cgaacaggat gtcgcccgcc
     4921 tgacgcagct cgcgcgagta gcggtcgcac agctcgacga tgtcgtcctc gcgcgtgacg
     4981 cggtgggtgc ggatcgggat gcgacgccag cgccggccat caaccgtgat caggggattg
     5041 cgctgtttgg ccatggccgc ctccggctcc ggattgcgtc agatctcgac cgtgaacagc
     5101 tcgtcgtcga tctcgccggc ggccacccgc gtgacggggc cggtcatcgt gatcgcgtaa
     5161 tcctcggcga tctgaatgcg cagattcccc cccggcatgt ggacagtgat gtcgcggtcg
     5221 cacagcccca gccggtgcgc gacggcggcc gccgcgctgg agctgctgcc cgaggcgagc
     5281 gtgtagccgg ccccccgctc ccagatctcg atgcggatgt tattgcggtc gagcacctgc
     5341 aggaactgga cgttcgtccg attgggaaaa atggggtggt tctccagctg gggccccagt
     5401 gcgcgggcct cctccgcgct gacctcgtcg cggacgacga cgcaatgcgg attgccgatc
     5461 gtggcggcgt tgtaggtgac aatcatctcc ccggcggtca gttgctggcc gaggacttcg
     5521 ccggcgacgc cgctgacggg aatttgcgcg gcgtcgaagc tgacccggcc catttcgacc
     5581 gtcaccctcg cgccgccggc gttcacctcg cagcggaccc ggccgcccgg ggtatccacc
     5641 gtaaacgggc gaccgggttg cacgcggccg ctgtcgagca gaaagcgcga aaaaatccgc
     5701 aagccgttgc cgctcttctc cgcctcgctg ccgtcgggat tccagatgcg caggccgaaa
     5761 tcgcccttgt tcgtcgccag cggcccgtgc aggatgccgt cggagccgac gccgtagttg
     5821 cgatggcaga tcttgcgcac cgcggtcggc tcgagccgct cgatcccgtc ggcggggtcc
     5881 atgaccagat aatcgttgcc cagggcgtga tacttgtgaa aacgcatgac ggaccggttc
     5941 caaggccagg gtttacggat ggggacgcga gagggcatgc tggaggcggc cggagactcc
     6001 ccaaacaggc gcagcctagc atggcgccgg ggtctttgca aaggcggggg cggggccgag
     6061 gtgaccggcc ggcggctcgg ttggatccat catccccgct gcaaccggca ggagatttgc
     6121 tcgcatcggc gcaagggagg cgaacttgcc ggcaacgatg aaccgcaacc ttctgttcgc
     6181 gacgctgacg gcgggcctgg cgctggccgg cgtgatcgcc gcccagaccg ccgcgccgcg
     6241 cctggagcgg ccgctgccca aaaccatcat ccgcggcaat ctgcacgtcg acggcgaacg
     6301 gacatggaac gcccagcatg tcgtgattca cggtgatctg atcctggaag agggcggccg
     6361 gctggaagtg aacgacagcg tcgtggaact ggtcggcgag gccgccggcg accgggtcat
     6421 ccgctggcgc ggcggcgccc tgctgacgat cgattccacg ctggggggga cgcccggaac
     6481 cgacgacgcg ccctccgcgc cggccgacct gctgctgctc gacggcgact ggggcaccac
     6541 ccggacgacg ctgcaacaca tcggcgaact gcgcgtgggc ggcgccccgc ccggcgggca
     6601 cctgtgggga acaggcctga ccgcgcgcga tcaggtcggg ccggttctgc tgagcggcgc
     6661 cggccggatt gaccttgtcg acagcgatgt gcggattgag ccggcaccgt agaatcgtct
     6721 attaaccacc gagtgggcga tccttaacgg ccccacgtcc gccgaccgcg atggcacgcc
     6781 gttggctttc cgactggggg acgcgattca cgggcgcgcc gtcccgttcc atccccccct
     6841 taaggaccca tactcatggc cacgcggaag gccgacgcga tcatcatcgg cagcggccag
     6901 gccggcgtcc ccctggcgct gaaatgggac cgaacgggca agaaggtcat tctgttcgag
     6961 aagggcgccc tcgggggaag ctgcgtcaat tacggctgca cgccgtccaa ggcctttctg
     7021 gcggcggcgc accgggcgca gagtgtccgc tcggcccgcg aactgggcgt ttacgccgac
     7081 gttcgtgtgg agttcgccga ggtcatgaag cgggtgcgcg acgtgcgtgc ggagtggaac
     7141 gaaggggtcg ggcgcaattt caagaacagc ggcatcgagc tgattgaggg cgtggcgcgg
     7201 ttcgccggcg aacgccgcgt cgaggcccag accgccgccg gaaccgaaac cgccgaggct
     7261 ccgctgattg tcatcgatac cggctccgcc ccccggatcc ccgccatccc cggtctggag
     7321 ggtacgccct tcctgacgaa catgaacttc ttcgatctgc gcgagcaacc ccgttgcctg
     7381 attgttctgg gcggcggcta tgtcggcctg gaactgggac aggggatgag ccggctgggg
     7441 agcgaagtgc acattct
//
LOCUS       DAOPCO010000197         7395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9053_length_7395_cov_5.906812, whole genome shotgun sequence.
ACCESSION   DAOPCO010000197 DAOPCO010000000
VERSION     DAOPCO010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9053_length_7395_cov_5.906812"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..2596
                     /gene="hypF"
                     /locus_tag="PLS90_12505"
     CDS             224..2596
                     /gene="hypF"
                     /locus_tag="PLS90_12505"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012596808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPK03266.1"
                     /translation="MVLPAATESALSPHPPGPAPVRLRLAIRGAVQGIGFRPFLHRLA
                     RELDLTGWIANTGDGVVAELEGPADALAQALARVPAEAPPRAHIQSLEPTWLDAAGYG
                     DLVIRPSLATAPRTLIQPDIATCPQCLRELRDPADRRHRYPFINCALCGPRFTIIESL
                     PYDRPHTTMRGFTMCERCRAEYGDPSDRRFHAQPIACPACGPQLALWSTAGRVLAQRG
                     EALARAADLLRRGGILALKGLGGFQLLVDARDEAGVARLRRLKRREAKPFAIMAPDLA
                     AARRLCDIGPLEERLLRSSEAPIVLLDRREAAAGDIAPAVAPRNPTLGLMLPYTPLHH
                     LLLDELGFPVVATSGNRGEEPICIDNQEALERLGPLCDALLVHDRPIRRHADDSIVRI
                     VAGREQVLRRARGYAPLPIAIRRAAPPLLAVGGHLKNTVALARGGEVFLSQHIGDLET
                     PEARRAQEAAADDLARLLAVEPRRVACDLHPDYASTRFAHGRGLPVAEVQHHAAHAWA
                     CLAENDLEPPALAVCWDGAGWGPDGTVWGGEFLAVDAEDCRRMGTLRTFPLPGGEAAM
                     REGRRAALGLLWEMLGPRLFDQSALAPVRNLTASERINFAAMLRRGLNSPRTSSAGRL
                     FDAAAALLEVCSVSRFEGQAAMELEFAARRAGPPPPAYPFRCVRSGGAAAELDWRPTF
                     AALLAEHAAAQSPAIPAARFHATLAAMIVAMAQREGRERVLLTGGCFQNRLLLELAIA
                     GLRRAGFRPYWHQRVPPGDGGLALGQAVAVARQANGEEGDGCASASRVKS"
     gene            2566..2802
                     /locus_tag="PLS90_12510"
     CDS             2566..2802
                     /locus_tag="PLS90_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPK03267.1"
                     /translation="MCLGIPGEILSIEEAQDELRRRGRVRFGGIVKEASLACVPEARV
                     GDYVIVHAGIAISRIDEAEAQAVFALLEEMEQRE"
     gene            2851..4002
                     /gene="hypD"
                     /locus_tag="PLS90_12515"
     CDS             2851..4002
                     /gene="hypD"
                     /locus_tag="PLS90_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPK03268.1"
                     /translation="MKYLDEYRDADAVRRLAAAIGRAATRPWTIMEICGGQTHAILRF
                     GLDELLPPEITLIHGPGCPVCVTPVELIDKALAIAARPGVIFCSFGDMLRVPGSRGAD
                     GRAADLMAVRAAGGDVRVVYSPLDAVALARANPSREVVFFAIGFETTAPANALAAALA
                     AREGLVNFSLLVSHVLVPPAISAILGSPDCRVQGLLAAGHVCAVMGAAACRPIAARHR
                     VPVVVTGFEPVDLLDGVLRCIRQLEAGRAEVENAYARAVADEGNPHARRRMEEVFEVC
                     DRSWRGLGRIPRSGLRLRSAYAAFDAERRFGVEAPGAGEPAECLSGLVLQGLRRPTDC
                     PAFATRCTPECPLGAPMVSAEGACAAYFRYRPSHSRQDRGTILKRGAAR"
     gene            3999..5030
                     /gene="hypE"
                     /locus_tag="PLS90_12520"
     CDS             3999..5030
                     /gene="hypE"
                     /locus_tag="PLS90_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPK03269.1"
                     /translation="MTCPVPNQDYDRILLAHGGGGRLTQRLLDRLFRPAFDNELLATR
                     HDGAALALGADRLAFTTDSYVVRPLFFPGGDIGRLAVCGTVNDLAMCGARPLWLSAGF
                     ILEEGLAVDDLRRVVESMRRAASDARVQLVTADTKVVDRGKGDGIYINTAGLGRIIAR
                     EPIGPASVRPGDAVLLSGDIGRHGITIMAARDGLAFESPLESDCAPLHEPVLELIADG
                     LPIHCLRDLTRGGLAAGLIEIAQTTGVDFEIDEEAIPIVDAVRGASELLGLDPLNIAN
                     EGRFLVILPERNADAALATLGRCDVARGACRIGRVTGAGRGETILRSRRGTRRVLDLF
                     SGEQLPRIC"
     gene            complement(5048..5515)
                     /locus_tag="PLS90_12525"
     CDS             complement(5048..5515)
                     /locus_tag="PLS90_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPK03270.1"
                     /translation="MKDMDKRVARLKPIEQMKLADETLKQIAACAQEVKFKEGAYVFR
                     ESEKAEQFYILEEGRVALELQGNAPAPMLIQTLGPGDVLGWSWLVEPQRWSFDARCLE
                     PVKAVVFDGEKIKRLCAKDEHLGYQLFRTFTAIIAGRLEATRRELVDLYGVQI"
     gene            5780..>7395
                     /locus_tag="PLS90_12530"
     CDS             5780..>7395
                     /locus_tag="PLS90_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPK03271.1"
                     /translation="MKLLFSFAFGLGALLLAACAAGPRPVTSHIPAHDGPTPWTHLDF
                     THDPADFQFLVMGDRTGGHRAGIFPKAVKSANQMRPEFVICVGDLIEGYTEDEAVMDA
                     MWDEFDAEVSQLEAPFFYVPGNHDVSNPAMERKWIERLGRPYYHFVYGNVLFLVLDSE
                     DPPRAFEGEHPHLDQAQLAWIRETLAANRDVRWTFLFLHKPVWTVESDTNWNDVEAML
                     ADRPYTVFCGHSHDYAKYERNGREYYNLATTGGGSRLRGHDVGEFDHITWVTMTDAGP
                     RVAPIELDGILDNDIIHADFKAVVDGVRDRVRVICDPVVAETQAFTQIDSTVRMTNES
                     PVAVDVDMVFRNPTELRLSRTAFTAHLHPGEQREWPVTFQSDQPVQVDEVGLIPYDYT
                     VRFSVPGQEGERRIEASGTTGVLLSYPIPRTEVSKTIDGDLSDWTSLPLAKPDPSPVR
                     EALAPVPVPPADLSYRFGVEHDDHYLYIAVDVTDDYASTHEAEHKWWMDSTVVRIDAR
                     PRERRFRGGREYEDYLVIVQNPGDSEREAYR"
BASE COUNT         1072 a         2557 c         2605 g         1161 t
ORIGIN      
        1 actgagaaag ccgcccgcgc cgctgggcaa ttggaatccg tccgatctgt ccgatcccac
       61 ggatccgacg gatgtcgatt tcagcgccta tagaagataa aaaacttctc ttatcttgaa
      121 tttgcgcccc atccatgtca tctttccatc agcggccccg ccgcccggac cgcgacgccc
      181 tcctcgtcgc ggcgccggtc ggattccaaa ggatcggcgg ctgatggtgc tcccagcggc
      241 gaccgaatcg gccctttcgc ctcacccgcc cggccccgcg ccggtccggc tgcggctggc
      301 gatccgcggc gcggttcagg gcatcggctt ccgtccgttc ctccatcgcc tggcccgtga
      361 actcgacctg accggctgga tcgccaacac cggcgacggc gtcgtggccg agctggaagg
      421 cccggccgac gcactggccc aggcgctcgc gcgcgttccg gccgaagccc ccccgcgcgc
      481 ccatatccag agtctggagc cgacgtggct cgacgccgcc ggctacggcg acctggtgat
      541 ccggcccagc ctggcgacgg cgccgcgcac gctgatccag cccgacatcg ccacctgccc
      601 gcagtgcctg cgcgagttgc gcgacccggc cgaccggcgc catcgctacc cgttcatcaa
      661 ctgcgcgctc tgcggtccgc ggttcacgat catcgagtcg ctcccctacg accggcccca
      721 tacgaccatg cgcggcttca cgatgtgcga gcgctgccgc gccgaatacg gcgatccgtc
      781 ggatcggcga ttccatgcgc agccgatcgc ctgcccggcg tgcgggccgc aactggcgct
      841 ctggagcacc gcggggcggg tgctggccca gcgcggtgag gctctggcgc gggccgccga
      901 cctgctgcgg cggggcggca tcctggcgct caaggggctt ggcggttttc aactcctcgt
      961 cgatgcgcgc gatgaggcgg gggtcgcccg gctgcggcgg ctgaagcggc gcgaggccaa
     1021 accattcgca atcatggcgc ccgatctggc ggcggcgcgg cggctgtgcg acatcggccc
     1081 gctcgaggag cggctgctcc gttcaagcga ggcgccgatc gtgctgctcg accgtcgcga
     1141 ggcggcggcc ggcgacatcg ccccggccgt cgcgccgcgc aatcccaccc tcggcctgat
     1201 gctgccctac acgccgctgc atcacctgct gctggacgaa ctggggtttc ccgtggtggc
     1261 caccagcggc aaccgcggcg aagaaccgat ctgcatcgac aaccaggagg cgctggagcg
     1321 gctggggccg ctatgcgacg cgctgctggt gcacgaccgg ccgatccggc gccacgcgga
     1381 cgattcaatc gtccgcatcg tggcggggcg cgaacaggtg ctgcgccgcg cgcggggtta
     1441 tgcgccgctg ccgattgcga tccgccgcgc cgcgccgccg ctgctggcgg tcggcggcca
     1501 cctgaagaac accgtggcgc tcgcgcgcgg cggcgaggtc tttctcagcc agcacatcgg
     1561 cgacctggag acgcccgagg cgcgccgcgc gcaggaagcg gcggccgacg acctggcgcg
     1621 cctgctggcc gtcgaaccgc gccgagtcgc ctgcgacctg catcccgact atgcctcaac
     1681 ccgcttcgcc cacggccgcg ggctgcccgt cgcggaggtc cagcaccatg cggcgcatgc
     1741 ctgggcctgc ctcgccgaaa acgacctgga gccgcccgcg ctggccgtct gctgggatgg
     1801 cgccggctgg gggcccgacg ggacggtctg ggggggtgaa ttcctcgccg tggatgcgga
     1861 agactgccgc cgcatgggaa cgctgcggac cttcccgctg ccggggggcg aggccgcgat
     1921 gcgcgagggc cgccgcgccg ctctgggcct gctgtgggag atgctgggcc cgcgcctgtt
     1981 cgaccaatcg gcgctggcgc cggtgcggaa cctgacggcg agcgaacgga tcaacttcgc
     2041 ggcgatgctg cggcgggggc tcaactcgcc gcgtacatcg agcgccggcc ggctgttcga
     2101 cgccgcggcc gccctgctcg aggtctgttc ggtcagccgg ttcgagggtc aggcggcgat
     2161 ggaactggag tttgccgcgc ggcgcgcggg gccgccgccg cccgcctacc cgttccgctg
     2221 cgtccggtcc ggcggcgcgg cggcggagct ggactggcgg ccgacgttcg cagcgctgct
     2281 ggcggagcac gccgcggcgc aatccccggc gatccccgcg gcgcggttcc atgcgaccct
     2341 ggcggcgatg atcgtcgcga tggcccaacg ggaaggccgg gagcgggtcc tgctgaccgg
     2401 cgggtgcttc cagaaccggc tgctgctgga gttggcgatc gccggcttgc gccgggccgg
     2461 gttccggcct tactggcatc agcgcgtgcc gccgggcgat gggggcttgg cgctcggtca
     2521 ggcggtcgct gtcgcccgcc aggcgaatgg ggaggagggg gacggatgtg cctcggcatc
     2581 ccgggtgaaa tcctgagcat tgaagaggcc caggacgaac tgcgccggcg cggccgggtg
     2641 cgcttcggcg gcatcgtcaa ggaagccagc ctggcctgcg tccccgaagc ccgcgtcggc
     2701 gactacgtga tcgtccacgc cgggatcgcc atcagtcgca tcgatgaggc cgaggcccaa
     2761 gcggtgttcg cgttgctgga ggagatggag cagcgggaat aaaggggaac gtgcggcggg
     2821 tttcgagagc cgaggacgag ggacggaatc ttgaagtacc ttgacgagta ccgcgacgcg
     2881 gatgccgtcc gccgcctggc cgcggccatc ggccgcgccg cgacgcgccc gtggacgatc
     2941 atggagatct gcggcggtca gacccatgcg attctgcgct tcggcctgga tgaactgctg
     3001 ccgccggaaa tcacgctgat ccacggcccc ggctgcccgg tctgcgtgac gccggtcgag
     3061 ctgatcgaca aggcgctggc gatcgccgcg cgccccggcg tcatcttctg ttcgttcggc
     3121 gacatgctgc gggtgccggg ttcgcgcggc gcggacggcc gcgccgccga cctgatggcg
     3181 gtgcgggcgg ccggcggcga cgtgcgcgtc gtctacagcc cgctggacgc ggtggcgctg
     3241 gcgcgcgcca atccgtcgcg cgaggtcgtc ttttttgcga tcggtttcga aacgacggcg
     3301 ccggccaatg cgctggcggc cgcgctggcg gcgcgcgaag ggctggtgaa tttctcgctg
     3361 ctggtttcgc atgtcctcgt cccgccggcg atctcggcca tcctcggctc gcccgattgc
     3421 cgggtgcagg ggctgctggc cgccggccac gtctgcgccg tgatgggcgc cgcggcctgc
     3481 cggccgatcg ccgcgcgcca tcgcgtgccg gtcgtcgtga ccggtttcga gccggtcgac
     3541 ctgctcgacg gcgtgctgcg ctgcatccgg caactggagg cgggccgcgc cgaggtcgag
     3601 aatgcctacg cccgcgccgt ggccgacgag ggcaatccgc acgcccggcg ccggatggag
     3661 gaggtgttcg aggtctgcga ccgctcatgg cggggcctgg gccggattcc gcgcagcggc
     3721 ctccggttgc ggtccgccta cgccgcgttc gatgccgagc ggcggttcgg cgttgaagca
     3781 ccgggcgccg gggaaccggc cgagtgcctc agcgggctgg tgctgcaggg cctgcgccgg
     3841 ccgaccgact gcccggcgtt cgcgacacgc tgcacgccgg agtgcccgct cggcgcgccg
     3901 atggtctccg ccgagggggc ctgcgcggcc tatttccgct atcgcccgag ccattcccga
     3961 caagatcgag ggacaatatt gaagcgaggc gccgcgcgat gacctgcccg gttccgaatc
     4021 aggattacga ccgcatcctg ttggcgcacg gcggcggcgg ccggctgacg cagcggctgc
     4081 tggaccggct gttccggccg gcattcgaca acgaactgct ggcgacgcgc cacgacggcg
     4141 cggcgctcgc gctgggcgcc gaccgcctgg ccttcacgac cgattcctac gttgtccgcc
     4201 cgctgttctt cccggggggc gacatcggcc ggctggccgt ctgcggcacg gtgaacgacc
     4261 tggcgatgtg cggggcgcgg ccgctctggc tcagcgcggg gtttattctg gaggaaggcc
     4321 tggccgtcga tgacctgcgc cgcgtggtgg aatcgatgcg gcgggcggcg agcgacgcgc
     4381 gcgttcagct cgtgacggcc gacaccaagg tggtggatcg gggcaagggc gacgggattt
     4441 acatcaacac ggccggcctc ggccgcatca tcgcgcgcga gccgatcggc ccggcgtcgg
     4501 tccggcccgg cgacgcggtc ctgctcagcg gcgatatcgg ccgccacggg atcacgatca
     4561 tggccgcgcg cgacgggctg gcgttcgaat cgcccctgga aagcgactgc gccccactgc
     4621 acgagccggt gctggagctg atcgccgacg ggctgccgat ccactgcctg cgcgatctga
     4681 cgcgcggcgg cctggcggcc gggctgattg aaatcgccca gacgaccggc gtggacttcg
     4741 agattgacga ggaggcgatt ccgatcgtgg acgccgtgcg cggcgccagc gagctgcttg
     4801 ggttggaccc gctcaacatc gccaacgagg gccggttcct ggtgatcctg cccgagcgaa
     4861 atgccgacgc ggcgctggcg acgctcgggc ggtgcgacgt cgcgcgcggg gcgtgccgca
     4921 tcggccgcgt gacgggcgcc ggccgaggcg aaaccatcct gcgcagccgg cgcggaaccc
     4981 gccgcgtgct ggacctgttc agcggcgagc agttgccgcg gatctgctga gccgcgcgcc
     5041 ggcgccgtca gatctggacg ccatagaggt cgaccagttc gcggcgcgtc gcttccagcc
     5101 gcccggcgat gatcgccgtg aacgtgcgga agagctgata gcccaggtgc tcgtccttcg
     5161 cgcacagccg cttgatcttc tcgccgtcga agacgacggc cttgaccggc tccaggcagc
     5221 gggcgtcgaa gctccagcgc tggggctcga ccagccagga ccagccgagg acgtcgcccg
     5281 gcccaagcgt ctggatcagc atcggggcgg gggcattgcc ctgcagttcg agcgcgacgc
     5341 ggccctcctc gagaatgtag aactgctcgg ccttttcgct ttcgcggaag acgtaggcgc
     5401 cctccttgaa tttcacctcc tgcgcgcagg cggcgatctg cttgagcgtc tcgtcggcga
     5461 gcttcatctg ctcgatcggc ttgagtctgg caacgcgctt atccatgtct ttcatgagaa
     5521 acgtctccct ttcagagcaa gcccggggtg gagatgggcc gctgagtttc ggccggcgtc
     5581 cggcatgtcg tcccgcttgg gttcaggaaa gcacagcggg gcgggatgac gcaaccaaaa
     5641 ataatacgcc ggagtcttta ataaccaatc gagttctgtt ctgagcggca ggatggcgcg
     5701 tgggcgaccg acggacttga cccgtttcag ccggagcggg aagatagggc gacagttcgc
     5761 cgaggttcac gcaattatca tgaaactttt attctcgttt gcgtttggcc tgggagctct
     5821 gctgctggcg gcgtgcgccg ccgggccgcg ccccgtcacc agccacatcc ccgcccacga
     5881 cgggcccacc ccctggacgc atctggactt cacccacgac ccggccgact tccagttcct
     5941 cgtcatgggc gaccgcaccg gcggccaccg cgccggcatc tttcccaagg ccgtcaaaag
     6001 cgccaaccag atgcgtccgg agttcgtcat ctgcgtcggc gacctgatcg agggctacac
     6061 cgaggacgaa gccgtgatgg acgcgatgtg ggatgaattc gacgccgagg tttcccagct
     6121 cgaagcgccc ttcttctacg tccccggcaa ccacgacgtt tccaatcccg ccatggagcg
     6181 caagtggatc gagcggctgg gacggccgta ctaccatttc gtctacggca acgtcctgtt
     6241 cctggtgttg gactccgagg acccgccgcg cgcgttcgaa ggggaacatc cgcatctgga
     6301 tcaagcgcag cttgcctgga tccgcgagac gctcgcggcg aatcgcgacg ttcgctggac
     6361 cttcctcttc ctgcacaagc cggtttggac cgtagagagc gacaccaact ggaacgacgt
     6421 cgaggcgatg ctggccgacc gtccgtacac ggttttctgc ggccattcgc atgactacgc
     6481 caaatatgaa cgcaacgggc gcgaatatta caacctggcc acgacgggcg gcggctcgcg
     6541 gctgcgcggc cacgacgtcg gcgaattcga tcacatcacc tgggtgacga tgaccgacgc
     6601 cggcccgcgc gtcgcgccga tcgaactcga cggcatcctc gacaacgaca tcatccacgc
     6661 cgacttcaag gcggtcgtcg atggggtgcg cgatcgggtg cgcgtgatct gcgatcccgt
     6721 cgtcgccgaa acccaggcct tcactcaaat tgactccact gtccggatga cgaatgagtc
     6781 gcccgtcgcc gtcgacgtcg acatggtctt ccgcaacccg accgaactgc ggctgagccg
     6841 caccgcgttc acggcgcacc tgcatcccgg cgagcagcgg gaatggcccg tcacgttcca
     6901 gagcgaccag cccgtccagg tcgacgaggt cggcctgatt ccctacgact acacggttcg
     6961 cttcagcgtg ccgggtcagg agggggagcg gcggatcgag gcgtcgggga caaccggcgt
     7021 gctgctgtcg tatccgatcc cgcgcacgga ggtgagcaaa accatcgacg gcgacctgag
     7081 cgactggacc agcctgccgc tggccaaacc cgatccctcg ccggtgcgcg aagcgcttgc
     7141 ccccgtgccg gtccccccgg cggatctgag ctaccgcttc ggcgtcgaac acgatgacca
     7201 ttatctctat atcgccgtgg atgtgaccga cgattacgcc tcgacgcacg aggccgagca
     7261 caagtggtgg atggacagca cggtggtgcg gatcgacgcg cggccgcgcg agcggcgttt
     7321 ccgcggcggc cgcgaatacg aagattacct ggtgatcgtg cagaatccgg gcgacagcga
     7381 acgggaggct taccg
//
LOCUS       DAOPCO010000198         7264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9232_length_7264_cov_4.913303, whole genome shotgun sequence.
ACCESSION   DAOPCO010000198 DAOPCO010000000
VERSION     DAOPCO010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9232_length_7264_cov_4.913303"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLS90_12535"
     CDS             <1..274
                     /locus_tag="PLS90_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03272.1"
                     /translation="TLHLEGLRNPFPTEITILQLRFRGEERTFEQPLPPTWFHLEIPI
                     QIDRPLEQPLLELRHPVYSLEVYQPEAGDPRTVGFLLHAIYVTPPR"
     gene            complement(284..1096)
                     /locus_tag="PLS90_12540"
     CDS             complement(284..1096)
                     /locus_tag="PLS90_12540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPK03273.1"
                     /translation="MLPLRDNIPPRTRPLANYLLILVTSVCFIGQFRAGDGEAGQRIV
                     ETFGLIPARVIYPEAALLMPEERVVMTRLGPLVEQTTRRIPPLPFSPLWTFLTSIFLH
                     GGLLHFLGNMWFLFIFGDNVEDRLGHARYLLFYLACGVLAGLAHFSFQMESIVPTIGA
                     SGAIAGVMGAYLVLYPHARVLAIVPVFVLLYTVVVPAPIFLGVWFLIQLVQGAIGLGS
                     LEMEGVAWWAHIGGFAAGIAAVIVLKALGQLRPPVKHIIPGTRHMRGIRARF"
     gene            complement(1100..1765)
                     /gene="elbB"
                     /locus_tag="PLS90_12545"
     CDS             complement(1100..1765)
                     /gene="elbB"
                     /locus_tag="PLS90_12545"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HPK03274.1"
                     /translation="MKTVGVCLSGCGYLDGAEIQEAVLTLLAIDQAGARAYCFAPDKE
                     QSDVIDHLTETAMPGKVRNVLTESARIARGKIENLDRVHAADLDALVFPGGYGAAKNL
                     CTFAAEGAECRVDPEVERLVREVVDTRRPIAAICIAPVIVARVLGQLGLHPRLTLGTD
                     AGLLAALEAMGAEPVQCGVTECVEDEPFRIVSTPAYLLGQGPGEVYQGIRGAIERLLA
                     MTG"
     gene            complement(1815..2012)
                     /locus_tag="PLS90_12550"
     CDS             complement(1815..2012)
                     /locus_tag="PLS90_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03275.1"
                     /translation="MAIYERMPALDPENVGGWNNYAEARGEARPDHPLLRRALATHPA
                     DARIQAVARQWGASPAQRDPE"
     gene            complement(2030..2308)
                     /locus_tag="PLS90_12555"
     CDS             complement(2030..2308)
                     /locus_tag="PLS90_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03276.1"
                     /translation="MDIEQLARFLIRGLRKVILVSAGGWPGFALHNTWGFRIVSSAPS
                     FSGAPANGGKATDAGPITPMPPPGIRAIRPLLSALAALIFMNMAVVIG"
     gene            2647..2817
                     /locus_tag="PLS90_12560"
     CDS             2647..2817
                     /locus_tag="PLS90_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03277.1"
                     /translation="MSREESPRKLSMTELIGEMNQAAENGRDDPNAKKKPHPGKDPKP
                     DNPHPCGNGNGG"
     gene            complement(2983..5475)
                     /locus_tag="PLS90_12565"
     CDS             complement(2983..5475)
                     /locus_tag="PLS90_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPK03278.1"
                     /translation="MIDKTPYTPGMREVLKLSKAEAGRLGHDYIGPEHYLLGVIRKGD
                     GLAIQTLMNLDVDLEDVKMELERMVEVGKGPTVGLFSPNADAKRVLETSKQIANEMKH
                     GWVGTEHLLLALIKEEHTLASKCLRQFGVDYEKAKKEVITVIEGSSTASTKSKESFEK
                     SKTPALDMFGRDLTQLAKEAKLDPVIGREEEIERILQILCRRTKNNPILLGEPGVGKT
                     AIVEGLAQKIVSGDIPELLRDKRLLSLDLAAIVAGTKYRGQFEERLKAIMQEIRRNPD
                     VLIFIDELHTLIGAGAAEGAIDASNMLKPALSRGEIQCIGATTLEEYRKYIEKDGALE
                     RRFQSITIDPPTIDQTVQILKGLRSRYEQHHNVIISDEAIEQAAVLSDRYVTDRFLPD
                     KAIDVIDEAGARARLQATSKPQEIKDLEQEVAELDEQLRVLAHKQEFEKCAELKEKKE
                     QIRQRIDGLMREWGKAKESKENVQVIGEKNVAYIVSKWTGIPVMRLEEEETQKLLRME
                     EELTKRVVSQSDAISAIAKAVRRARSGLKDPKRPTGSFIFLGPTGVGKTELAKALAEF
                     LFGDEDALIRIDMSEYMEKYSVSRLLGAPPGYVGYEEGGQLTEKVRQKPYSVVLLDEI
                     EKAHPDVYSLLLQVFDDGRLTDSWGHIVDFRNTVIILTSNIGTRRLKKSGTLGFNAGD
                     EMLDYEGLKGKVLGELKKAFNPEFLNRLDEAIVFKSLEKGDIEKIVDIMMDRLNDRLV
                     DRGIVIHLDQEAKDFLVEQGYDSEYGARPMRRAIQKYIEDPLSSLMITGEVPDGADVN
                     ATRKKDADELEFTVKVNSGQREKVGAKSAGGD"
     gene            complement(5746..6789)
                     /locus_tag="PLS90_12570"
     CDS             complement(5746..6789)
                     /locus_tag="PLS90_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HPK03279.1"
                     /translation="MAEHEMLDEWIEQEGPMTEVVVSSRARLARNLKGVPFSPRANES
                     QRQFVAQRVRQAFEQHDELAGYHQLDLASLPADERVRLRESHLISREMEKGGVGRVVL
                     LGPRMDTSVMVNEEDHLRMSVLVSGFRVEEAHRRILAMEEKLEQSLPFAYSEKFGYLT
                     ACPTNTGTGLRLSVMLHLPALVMTEQIERTMSPLGSFGLVVRGAYGEHSEHTGDLFQI
                     SNEVTLGKTEQQIMEILNEVVRQVIDRELAARQALREQARIKLEDIVCRAVGLLSSAR
                     MIDSNEATALLSRARLGVGQSWCSIPLDHARLSRLFIEVQPAHLQFREETQSPEERDL
                     VRAEMLRAMFGEN"
BASE COUNT         1261 a         2597 c         1993 g         1413 t
ORIGIN      
        1 caccctccat ctggaaggcc tgcgcaaccc cttcccgacc gaaatcacga tactccaact
       61 ccgcttccgg ggcgaagagc gaaccttcga gcagccgctg cccccgacct ggttccatct
      121 cgaaattccg atccagatcg accggccgct ggagcagccg ctcctcgaac tgcgccaccc
      181 ggtctactct ctcgaggtct accagcccga agccggagac ccgcgaacgg tcgggttcct
      241 gctccacgcc atctacgtca ccccgccccg gtagccgctt tgttcagaaa cgcgcccgga
      301 tcccccgcat gtggcgcgtc cccgggatga tgtgtttcac gggcggccgc aattgaccga
      361 gcgccttgag cacgatcacc gcggcgatgc ccgccgcgaa cccgccgatg tgcgcccacc
      421 aggccacccc ttccatctcc agcgatccca gcccgatcgc gccctgaacc agttgaatca
      481 ggaaccagac gccgagaaag atcggcgccg gcacgaccac cgtgtagagc agcacgaaca
      541 ccggcacgat cgccagcacc cgtgcgtgtg gatagagcac cagatacgcc cccatcacgc
      601 cggcgatggc gccgctcgcg ccgatcgtcg gcacgatgga ttccatctga aagctgaagt
      661 gcgccaaccc cgccagcacg ccgcaggcca ggtagaacag gagatagcgc gcgtgtccca
      721 gccggtcctc gacattgtcg ccgaagatga agagaaacca catgttgccc agaaaatgca
      781 gcaggccgcc gtgcagaaag atgcttgtga ggaacgtcca cagcgggctg aacggcagcg
      841 gcgggatgcg ccgtgtcgtc tgctcgacca gcggccccag ccgcgtcatc acgacccgct
      901 cctccggcat cagcagcgcc gcctcggggt agattacccg cgccggaatc aggccgaaag
      961 tctcgacgat ccgctggccc gcctcgccgt cgcccgcccg gaactgaccg atgaagcaaa
     1021 cgctggtaac cagaatcaac aggtaattag caagcggacg cgtgcggggc ggaatgttgt
     1081 cgcgcagcgg gagcatcgct cagcccgtca tcgccagcag ccgctcgatc gcgccgcgaa
     1141 tgccctgata cacctcgccc ggcccctgcc cgagcagata ggccggcgtt gatacgatcc
     1201 tgaacggctc gtcctcgacg cactcggtca cgccgcactg caccggctcg gcccccatcg
     1261 cctccagcgc cgccagcagc ccggcgtcgg ttcccagcgt caatcgcggg tgcaggccga
     1321 gctgcccgag cacgcgcgcc acgatcaccg gcgcgatgca gatcgcggcg atcggccggc
     1381 gcgtatcgac cacttcgcgc accagccgct ccacctcggg atcaacgcgg cattcggcgc
     1441 cctccgccgc gaacgtgcaa aggttcttcg ccgcgccata cccccccggg aaaaccagcg
     1501 cgtccaggtc ggcggcatgc acccggtcca ggttctcgat tttcccgcgg gcaatccgcg
     1561 cgctttccgt cagcacgttg cgcaccttgc ccggcatcgc cgtctcggtc aggtgatcga
     1621 tcacgtcgct ctgttccttg tccggcgcga agcagtaggc ccgcgccccc gcctgatcga
     1681 tcgccagcag cgtcaacacc gcctcctgaa tctcggcgcc gtccagatag ccgcatcccg
     1741 acaggcaaac tcccaccgtc ttcatctcgt ctctccttgc tcaggcagtt gatcgcgcgt
     1801 cgcgccttcc ccgctcattc cggatcgcgt tgggctggcg acgcgcccca ctggcgcgcc
     1861 acggcttgga tccgcgcgtc ggccggatgg gtcgccaacg cccgccgcag cagtggatga
     1921 tccggccggg cctcgccgcg cgcctccgcg tagttattcc aaccccccac gttctccggg
     1981 tcaagcgccg gcatccgttc gtagatcgcc atcgcctcgg ccggacgact cagcctatca
     2041 caaccgccat gttcatgaaa atgagcgcgg ccagcgcgct caacagcgga cggatcgccc
     2101 ggattccagg tggcggcatc ggcgtgatcg gccccgcgtc ggtggctttc cccccgtttg
     2161 ccggcgcgcc ggaaaacgat ggcgcgctgc tcactatgcg aaacccccac gtgttgtgaa
     2221 gcgcaaaccc cggccacccc ccggctgaaa ccaggatcac tttccgcaag ccgcgtatca
     2281 ggaagcgcgc cagctgttcg atatccaagc cgtgggttga attggccgcc gggcagccat
     2341 tcaacccacg gttaggttgc gcccgacgcg agaaccgatt tctcggttca atatttaaca
     2401 cgtttcggta atcccgcttt gccctgcgtc gcccctcaga tcccggatcg ccggtcgccc
     2461 tccggtcgcc ggcagccctt ctaataaaag gacttacgcc gccgagaaaa atgttttcct
     2521 tgaaattccc gcctcgacgc cgctgcccgc ttgacaagat cgaaactttg atttaagtaa
     2581 tttgcagcag aaggagaggg gatctcgttt gagttcgtta tcctttttct tcggaggaca
     2641 acagtcatgt cgcgcgagga gtcaccccgc aagctctcca tgaccgaact catcggcgag
     2701 atgaatcagg ccgccgagaa tggtcgcgac gacccgaacg cgaagaagaa gccgcacccc
     2761 ggcaaggacc cgaaacctga caacccccac ccctgcggca acggcaacgg gggctgagcc
     2821 ggctttctca aaataacgac aaacgaaccg ccccgaggcg aacgaagtat aagatcttaa
     2881 catcttataa cgtcggcccc acacgcaccc gcacgcgaaa cggcccgggc agtgccgccc
     2941 gggccgttcg cattccagca tccgctgacc ggccgtctgg tttcaatccc cgccggcgct
     3001 cttcgcgccc actttttccc gctggccgct gttgaccttc accgtgaact ccagctcgtc
     3061 ggcatccttc ttccgcgtgg cgttcacatc ggccccgtcg ggcacctcgc cggtgatcat
     3121 cagcgagctg agtggatctt cgatgtattt ctggatcgcc cgccgcatcg gccgcgcgcc
     3181 atattcgctg tcgtagccct gctcgaccag gaagtccttg gcctcctgat ccaggtggat
     3241 cacaatgccg cgatccacca ggcggtcgtt cagccgatcc atcatgatgt cgacgatctt
     3301 ctcaatgtcg cctttttcga gcgacttgaa gacgatcgcc tcatccagcc ggttcaggaa
     3361 ctcagggttg aacgccttct tcaattcccc gagcaccttc cccttcagac cctcgtagtc
     3421 gagcatctcg tcgcccgcgt tgaagccgag cgtcccggac ttcttcaacc ggcgcgtccc
     3481 gatgttgctc gtcaggatga tcaccgtgtt gcggaagtcg acgatgtggc cccacgaatc
     3541 ggtcaggcgg ccgtcgtcga agacctgcag cagcaggctg tagacatccg ggtgcgcctt
     3601 ctcaatctcg tccagcagca cgaccgaata gggcttctgg cgcaccttct ccgtaagctg
     3661 gccgccctct tcgtaaccga catagcccgg aggcgccccg agcagacggc tgacggagta
     3721 tttctccatg tattcgctca tatcgatccg gatcagggca tcctcgtcgc cgaagaggaa
     3781 ctcggccaat gccttggcca actccgtctt gcccacgccc gtcgggccca ggaagatgaa
     3841 actcccggtc ggccgcttcg gatccttgag ccccgaccgc gcgcgccgca ccgccttggc
     3901 gatcgccgaa atcgcgtcgc tctggctgac gacccgcttg gtcagctcct cttccatccg
     3961 cagcagcttc tgcgtctctt cctcctccag gcgcatcacc gggatgccgg tccacttcga
     4021 aacgatgtag gccacgttct tctcgccgat gacctgcacg ttctccttgc tttccttggc
     4081 cttgccccat tcgcgcatca ggccgtcgat ccgctggcgg atctgttcct tcttttcctt
     4141 cagttccgcg cacttctcaa attcctgctt gtgcgccagc acgcgcagtt gctcgtcgag
     4201 ctcggcgacc tcctgctcca ggtccttgat ctcctgcggc ttgctggtcg cctgcagccg
     4261 cgcgcgggct cccgcttcgt cgatcacatc gatcgccttg tcgggcagga accggtcggt
     4321 cacataccgg tccgacagca cggcggcctg ttcgatcgcc tcatccgaga tgatcacgtt
     4381 gtgatgctgc tcgtagcgcg agcgcaggcc cttcaggatc tggacggtct ggtcgatggt
     4441 cggcgggtcg atcgtgatcg attggaaccg ccgctccagc gccccgtcct tctcgatgta
     4501 cttgcggtat tcctccagcg tcgtggcgcc gatgcactgg atctcgccgc ggctgagcgc
     4561 cggcttgagc atgttggatg cgtcgattgc cccttccgcc gcgcccgcgc cgatcagcgt
     4621 atgcagttcg tcgatgaaga tcagcacgtc gggattgcgc cggatctcct gcatgatcgc
     4681 cttgagccgc tcctcgaact gcccgcgata cttcgtgccg gccacgatcg ccgccaggtc
     4741 gagcgacagc agccgcttgt cgcgcagcag ttccggaatg tcgcccgaaa cgatcttctg
     4801 cgccaggccc tccacgatcg ccgtcttgcc cacgcccggc tcgcccagca ggatcgggtt
     4861 gttcttcgtc cgccggcaga ggatctgcag aatccgctcg atctcctcct cgcggccgat
     4921 caccggatcc agcttcgctt ccttcgccag ttgcgtcagg tcgcgcccga acatgtccag
     4981 cgccggcgtc ttgctcttct caaacgattc cttgctcttg gtcgaggccg tgctgctccc
     5041 ctcgatgacc gtgatcactt ccttcttggc cttctcgtaa tcgacgccga actggcgcag
     5101 gcatttgctc gccagcgtgt gctcttcctt gatcagcgcc agcagcaggt gctcggtccc
     5161 cacccacccg tgcttcatct cgttggcgat ctgcttgctg gtctccagca cacgcttggc
     5221 atcggcattg ggcgagaaca gcccgacggt cggccccttg ccgacttcga ccatccgctc
     5281 cagctccatc ttgacgtctt ccaggtcgac gtcgaggttc atgagcgtct ggatcgccag
     5341 gccgtcgccc ttgcggatca cgcccagcag atagtgctcc ggcccgatat aatcatgacc
     5401 caggcggccg gcttcggcct tggagagctt gagcacctct cgcatccctg gtgtgtacgg
     5461 ggtcttgtcg atcatgatgt ttcatcctcc ccacccaaaa tgacctgtcg gctcggcctg
     5521 cgatccacgg gaacggtttt caaagtcagg ggggtgagcc actccttcca tggtctgcaa
     5581 tcacatcgat cagtcgttcc aaggttgtga tcctgatgcg gccctccatt atttcttctt
     5641 cggctgccgg aggggcgata atgaatcccg atcgacacaa aacctgctcg cccctgacat
     5701 tctatacctg cccgccaaaa gtgtcaaacc ggccacccat cgccctcagt tctctccgaa
     5761 catcgcgcgc agcatctcgg cccgcaccag gtcgcgttcc tccggcgatt gcgtctcctc
     5821 gcggaattgc agatgggcgg gctgcacttc aataaacagc cggctcaatc gcgcatgatc
     5881 caacggaatc gaacaccagc tttggccgac gcccagtcgc gcccgcgaca gcagcgccgt
     5941 tgcctcgttc gaatcgatca tgcgggcgga agacaacaaa ccaaccgcgc gacacacgat
     6001 gtcctcaagc ttgattcgtg cctgttcacg cagtgcctgg cgcgccgcca gttcgcggtc
     6061 gatcacctga cgcacgacct cgttgaggat ctccatgatc tgctgctccg ttttccccaa
     6121 cgtcacttcg ttggagatct gaaacagatc ccccgtatgc tcggagtgct ccccatacgc
     6181 cccgcggacc accagcccga agctgcccag cgggctcatc gtccgctcga tctgttcggt
     6241 catcaccagc gccggcagat gcagcatcac cgacagccgc aggccggtcc ccgtgttggt
     6301 cgggcacgcc gtcagatagc cgaacttctc cgaataggcg aacggcagcg actgctccag
     6361 tttctcctcc atggccagaa tccggcggtg agcttcctcg acgcggaatc ccgagaccag
     6421 caccgacatc cgcaggtggt cttcctcgtt caccatcacc gatgtatcca tccgcggccc
     6481 cagcagcacc acccggccga cgcccccttt ctccatctcc cgcgaaatga ggtggctctc
     6541 gcgcagccgg acgcgctcat cagccggcag cgaggccaga tccagttggt ggtagcccgc
     6601 cagctcgtca tgctgctcga aagcctgccg cacgcgttgc gccacgaact ggcgttgcga
     6661 ctcgttcgcc cgcggtgaaa acggcacccc cttcagattg cgcgccaggc gcgcgcggct
     6721 ggagacgacc acttcggtca tcggcccttc ctgttcgatc cactcgtcaa gcatttcgtg
     6781 ctcggccatg cgttcaggcg gtcctttcga cgaattagac agcggcgagc gcgacatgtt
     6841 gcgccgcatg cgagccgcgc ccgcgcgaag caggtcgttc atctgaaaat gaaccggaaa
     6901 tggagcagtg cacgatacaa atcgtccccc acgcccggtc cttttagcga ataatatacc
     6961 gccgcgcaag cccgccgtcg agggcatgag gcgttcggaa tcttcatttt acgatggctt
     7021 gccgttcgcc tgcgcgcttt cgccgggccg ggccgtggta gagttttgac acatccgctc
     7081 ctcggggacg agcttttttg acgcagccgc tacggcttcg gaatcgcagt tatcgactcg
     7141 ctcttttcca ccgatttcga tcgcgtcccc ccgatttttt ctttcaccgg ccctctcatt
     7201 ttgatggagc ggtcatcgat tgtgagattt cctgagcaca atgtgtgatt ttatgccgac
     7261 cggc
//
LOCUS       DAOPCO010000199         7262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9241_length_7262_cov_5.472319, whole genome shotgun sequence.
ACCESSION   DAOPCO010000199 DAOPCO010000000
VERSION     DAOPCO010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9241_length_7262_cov_5.472319"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="PLS90_12575"
     CDS             complement(<1..1323)
                     /locus_tag="PLS90_12575"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPK03280.1"
                     /translation="MATAPAPSTADYPAAALSPPGGAALRLLAYPFFFASGAAGLIYE
                     IVWTRMLLAIFGAGLYAVCTVLAAFMAGLALGSWLLGRVSDRLGRPLRTYGALEIGIA
                     LAGAAMPLLMNGVTAVDGWIYAHLGGQFGILTAARFILAFLLLLIPTTMMGATLPVLS
                     RFMVRRQAHLGLHVGSLYAVNTFGAVAGSFLAGFVLIASFGLLHSEWIAAGLNLFVAA
                     GSLALSLMIERRPLSPGGPPVAATAGAPAPPTDAPPPHAVQWVLWTALLSGAAALACQ
                     VLWSRSLVFSFEYLKNTTYSFSAMLTVFLAGLAVGAALIGLWIDHERQPLRLYGVLLV
                     LIGMAVLLSVTMLYSAGGLRIADPYDPETGILNWYLAVGNIMLQTFGVLGLPTILLGM
                     AFPAAARVVVQVGRVGGDVGRLYAYNTVGAILGALLAGFIIIPLFGLTF"
     gene            1604..2566
                     /locus_tag="PLS90_12580"
     CDS             1604..2566
                     /locus_tag="PLS90_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPK03281.1"
                     /translation="MEQNVQKVITLGQDEILAFCGEGDRKLRQIQSRMKARVVARGNE
                     LRVIGSTEEVDRAFEMINELLQIQRSHHQPLSDQQVQLAATHYESGREGSIREVFLDT
                     IPVPLKRRHVAPLTATQKIYIDAIRRHDVVFGTGPAGTGKTYLAVAMAVSKLIAGDVG
                     RIILVRPAVEAGESLGFLPGDLRAKLDPYLRPLTDALYEMLDADRLRQFIEDGIIEIA
                     PLAYMRGRTLNNSFIILDEAQNTSIEQMKMFLTRLGLDSQAVITGDPTQIDLPKGRTS
                     GLLHVQKILAGVDGIDFIHFSGNDVVRHALVQKIIRAYETHGQG"
     gene            2553..4958
                     /locus_tag="PLS90_12585"
     CDS             2553..4958
                     /locus_tag="PLS90_12585"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPK03282.1"
                     /translation="MAKDKSGSLRLDGTGSRPRLPQATAPGIIDPRAAAERRANRVKR
                     AVSVALWYAITVFVLGLVLRFEPYVTTVGEPARTDVVAPFSFEIIDATATEEARRRLY
                     EQKAQIWIRDTDAEGLARQQLNQFFDLVEESELDSAEDLRKFSDALFEQFDIALQATS
                     AAEFSASRLRRFRDPLSLSDVRRDLELVITDAYNNRCIVTDKPLFRAHAGAGVLRLYN
                     TRNLPEEIPNPETVLWYPDELQNFLLTKALPRYFPDADMATLRAAAAELLREIIEPNI
                     EYSRVKTDEKYVEDVRMLLEFPRRRRFAENESIVTRGEPLNALQATALGYLNDQRRLR
                     STIKLIGIVIITAVIFFAVGLYLRRFRRGLALDATTVALHVLPPLLAISIAHAMLLLG
                     LPEQQLTYWFPAGLIGMLASLLFSPQVAFVLVLVGALLTGLASEGQGLQFIVLALFGG
                     FAAALASRTVRARGEVLRVGLYIGIVNVITITLLAFVNSQSHPDPRNLLAGFLNGLVC
                     ALITLVFLVIIEWAFGIVTDLKLLELTGLKHPLIRQLEEKAPGSYQHVLNVVKLAEAA
                     ADAIGANYLLVRAGAYFHDIGKMLKPNYFSENQVTIDDKKAHSRLTPYMSVLIIKNHV
                     KEGMELGRKFGLPQQIIDFIPQHHGTDLIRYFYSEALRRYEESETMDPVREEDFRYPG
                     PKPQSIETAIVMLADTVEATAASKFTGGQVSEDELRRAVQTSITTKFNDGQFDECPLT
                     LAHLSQIREAFVRSLMARYHFRVAYPALPRRDQPPPPSGREARSEATSPSIAVIGPAA
                     SAT"
     gene            complement(5083..6699)
                     /locus_tag="PLS90_12590"
     CDS             complement(5083..6699)
                     /locus_tag="PLS90_12590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03283.1"
                     /translation="MPSPDAMGTAPWRARHGLLAVLLAAAAAPWLLWMLLFSTETYDE
                     RVHVNVGVVVWRQRNLSWDIVHPPGRAILAIPALLKRMPTPADPIDPLPGYGGEWTLR
                     SARCVTLALFLLWSLLFARIVGHWFTPATGLLAQALILFSPTLTPHAVLATNDVLVTM
                     VCFLLSAAALEWSRRESGRLALAVGLLGGIALLTKYTGLLWLGGMTVGMALWLGRRLA
                     PPRARGAASRGAVAAQVLLMLGLAIVLPALVYDFDGLGQPLASFHVRHGLLQLVGKLL
                     GWLPAPLPWPYWHGMDELLMSIQYRHTYFHGQWRHAAGFRPYFAMLLLAKPPLALHGM
                     LLLALVAVVRQRAGPSLRAACFLVLPPLALLLYGTFGSGIQIGIRHMLPLFPLLFAAA
                     AWGVLAFSRRAWRAVGLTLVLWFILLDLSLCPHQLGYFNPIVGGPAKAWRWFIDSNQD
                     WGQGGRAAKRFIDEFGQEIDTDPVAGKTTGWVLVATNQLVGITPEQGEKFRWLRENFE
                     PVDFIMPCFHLFHIPEDAWRNQTGDHRPRG"
     gene            complement(6699..>7262)
                     /locus_tag="PLS90_12595"
     CDS             complement(6699..>7262)
                     /locus_tag="PLS90_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPK03284.1"
                     /translation="QPIDQSIPRFKQVFGPLARRAADKGLKLAFENCDMGGDWASGDF
                     NIAHNPTAWEMMFDAVPGDTLGLEWEPCHQMVRLIDPLPQLRKWVGRIYHLHGKDATV
                     MWDVVREYGVDGPRPFAFHRTPGFGDSNWTDIISELRRAGYRGTIDIEGWHDAVWRGE
                     LEMTGQVHALNYLKHCRGGAFVPNPPV"
BASE COUNT         1313 a         2281 c         2616 g         1052 t
ORIGIN      
        1 gaaggtcagg ccgaacagcg ggatgatgat gaagccggcg aggagggcgc cgaggatcgc
       61 gcccaccgtg ttgtaggcat acagccggcc gacgtcgccg ccgacccggc cgacctggac
      121 caccacgcgc gcggcggcgg gaaacgccat cccgagcagg atcgtcggca acccaaggac
      181 gccgaaggtc tggagcatga tgttgccgac ggccaggtac cagttgagga tgcccgtttc
      241 ggggtcgtag ggatcggcga tgcgcaggcc gccggccgag tagagcatcg tgacggagag
      301 caggacggcc ataccgatca ggacgagcag gacgccgtag aggcgcaggg gctggcgttc
      361 gtgatcgatc cacaggccga tcaacgcggc gcccacggcc agcccggcca gaaagaccgt
      421 cagcatcgcc gagaaggagt aggtggtgtt tttgaggtat tcgaagctga agacgagcga
      481 gcggctccag aggacctggc aggcgagggc cgccgcgccc gaaaggagcg cggtccagag
      541 cacccattgc acggcgtggg ggggcggcgc gtcggtcggt ggggcgggcg cgccggcggt
      601 cgccgccacg ggggggccgc cgggagaaag cggtcggcgc tcgatcatca gcgacagggc
      661 cagactgccg gccgcgacga acagattcag ccccgccgcg atccattcgg agtgcagcag
      721 gccgaagctg gcgataagga cgaagccggc gaggaacgag cccgccaccg cgccgaacgt
      781 attgaccgcg tagagactgc cgacatgcag acccaggtgg gcctgacgcc gcaccatgaa
      841 ccgcgacagg accggcagcg tggcgcccat catggtggtg gggatcagga gcaggaggaa
      901 cgccaggatg aagcgggcgg cggtcaggat gccgaactgg ccgcccaggt gggcgtagat
      961 ccagccgtcg acggcggtga cgccgttcat cagcagcggc atggccgcgc ccgccagcgc
     1021 gatcccgatc tccagcgcgc cgtaggtgcg cagcggccgg ccgagccggt cgctgacccg
     1081 gcccagcagc caggagccga gcgccaggcc ggccatgaag gcggcgagga cggtgcagac
     1141 ggcgtagagt ccggcgccga agatggccag cagcatccgc gtccagacga tctcgtagat
     1201 caggccggcc gcgccggaag cgaagaaaaa ggggtaggcg agtagccgca gggcggcgcc
     1261 gccggggggg gacagcgcgg cagcggggta gtcggcggtg gacggagcgg gtgcggtggc
     1321 catcggtctg gagcgtttcg cccgtgcgga gataatgcgg tcggaaatcg agagttgcgc
     1381 cgccgaccgg ggacgggcga tcggagggaa cccgcacgcc cggcgagccc tccaggcctt
     1441 gtgcggcgaa cccctttccc gcttgacagc ccgaaaaggg gacccgtacg atgcaaccac
     1501 gggctgaatc gggcttgcgc ggagctgcct gccggtcgtt cccggaggcc cgtggaccgg
     1561 cgcccgtcag tcgcgggcaa agcgaggtga caagttaaac cgcttggagc agaatgtcca
     1621 gaaagtgatc acgctgggcc aggatgagat cctggcgttt tgtggggaag gcgaccgcaa
     1681 actgcggcaa atccagtccc ggatgaaggc cagggtcgtg gcgcgcggca acgagctgcg
     1741 cgtgatcggc tccaccgagg aggtcgaccg ggccttcgag atgatcaacg agctgctgca
     1801 gatccagcgc agccatcatc aaccgctgag cgatcaacag gtgcaactgg ccgccaccca
     1861 ttacgaatcg ggccgcgagg ggagcatccg cgaggtcttt ctggacacga ttccggttcc
     1921 gctcaagcgg cgacacgtcg ccccgctgac ggcgacgcaa aagatctaca ttgatgctat
     1981 ccggcgccac gacgtcgtct tcggcaccgg gccggcgggg accggcaaga cgtatctggc
     2041 ggtggcgatg gcggtctcca agctgatcgc cggcgacgtc ggccgcatca tcctggtgcg
     2101 cccggcggtg gaggcgggcg aaagcctcgg ctttttgccg ggggacctgc gcgccaagct
     2161 cgatccgtat ctgcggccgc tgaccgatgc gctctacgag atgctcgacg ccgaccggct
     2221 gcgccagttc atcgaggacg gcatcatcga aatcgcaccg ctggcctaca tgcgcggccg
     2281 gacgctgaac aacagcttca tcattctgga cgaggcgcag aacacctcga ttgaacagat
     2341 gaagatgttc ctgacgcggc tggggctgga ttcgcaggcg gtgatcacgg gcgatccgac
     2401 gcagatcgat ctgcccaagg ggcggacctc cggtctgctg cacgtccaga agatcctggc
     2461 cggagtggac gggatcgatt tcatccattt ttcgggcaac gacgtcgtgc gtcacgcgct
     2521 ggtccagaag atcatccgcg cctacgaaac ccatggccaa ggataaatcc ggctccctgc
     2581 gtctggacgg gaccgggtcg cgtccgcggc tgccgcaggc gacggcgccg gggatcatcg
     2641 atccgcgggc ggcggccgag cgccgggcca accgcgtcaa gcgcgccgtt tcggtcgcgc
     2701 tgtggtacgc catcaccgtc ttcgtgctgg ggctcgtgct ccgcttcgag ccgtacgtga
     2761 cgaccgtggg ggagccggcg cggaccgacg tcgtcgcgcc gttctcgttc gaaatcatcg
     2821 acgcgacggc caccgaggag gcccgccggc ggctgtatga acagaaggcg cagatctgga
     2881 tccgcgacac ggacgccgaa ggcctggcgc gccagcaact gaatcagttt ttcgatctgg
     2941 tggaggagag cgaactggac agcgccgagg atttgcggaa gttttccgac gcgctgttcg
     3001 aacaattcga tatcgcgctg caggcgacgt cggccgccga gttcagcgcg tcgcgtctgc
     3061 gccggttccg cgatccgctc agcctgagcg acgtccgccg cgacctggaa ctggtcatca
     3121 ccgacgccta caacaaccgc tgcatcgtga cggacaagcc gctgttccgg gcgcacgcgg
     3181 gggccggcgt gctgcggctg tacaacacgc gcaacctgcc ggaggaaatc cccaatccgg
     3241 agacggtgct gtggtatccc gacgagctgc agaattttct gctgaccaag gcgctgccgc
     3301 gatattttcc cgatgccgac atggcgacgc tgcgggcggc ggcggccgaa ctgctgcggg
     3361 agatcattga gccgaacatc gaatacagcc gcgtcaagac cgacgagaag tatgtcgagg
     3421 atgtgcggat gctgctggag tttccgcggc ggcggcgctt cgcggagaac gaatcgatcg
     3481 tcacgcgcgg cgagccgctc aacgcgctgc aggccacggc gctggggtat ctgaacgacc
     3541 agcggcggct gcgctcgacg atcaaactga tcggcatcgt gatcatcacg gcggtgatct
     3601 tcttcgccgt cgggctgtat ctgcggcggt tccggcgcgg gctggcgctg gacgccacga
     3661 cggtggcgct gcacgtgctg ccgccgctgc tggcgatcag catcgcccac gcgatgctgc
     3721 tgctcgggct gcccgagcag cagttgacct actggtttcc ggcggggttg atcgggatgc
     3781 tcgcctcgct gctgttcagc ccgcaggtcg ccttcgtgct ggtgctggtg ggggcattgc
     3841 tgacggggct ggcgagcgag gggcagggac tgcagttcat cgtgctggcg ctgttcggcg
     3901 gtttcgccgc ggcgctggcc agccgcacgg tgcgcgcccg cggcgaggtg ctgcgcgtcg
     3961 gcctctacat cgggatcgtg aacgtcatta cgattacgct gctggcgttc gtcaattcgc
     4021 agtcgcatcc cgacccgcgc aatctgctcg ccggcttcct caatgggctg gtctgcgcgc
     4081 tgatcacgct ggtcttcctg gtcattatcg aatgggcgtt cgggatcgtg accgacctca
     4141 agctgctgga gctgaccggt ctgaagcatc cgctgatccg gcaactggag gagaaggccc
     4201 ccgggtcgta tcagcacgtg ctcaacgtgg tgaagctggc cgaggcggcg gccgacgcga
     4261 tcggcgcgaa ctacctgctg gtgcgtgccg gggcgtattt ccacgacatc ggcaagatgc
     4321 tcaagccgaa ctatttctcc gagaaccagg tgacaatcga cgacaagaag gcgcactcgc
     4381 ggctgacgcc gtacatgagc gtgctcatca tcaagaatca tgtgaaggag gggatggagc
     4441 tggggcggaa attcggtttg ccgcagcaga tcatcgactt tatcccgcag caccacggga
     4501 ccgatctgat ccgctatttc tacagcgagg cgctgcgccg ctacgaggag agcgaaacga
     4561 tggatccggt gcgcgaggag gatttccgct accccggacc caagccgcag tcgatcgaga
     4621 cggcgatcgt gatgctggcc gacacggtgg aggcgacggc cgcgagcaaa ttcaccggcg
     4681 gccaggtgag cgaggacgaa ctgcgccgcg cggtgcagac gtcgatcacg accaagttca
     4741 acgacggcca gtttgatgaa tgcccgctga cgctggcgca cctgagccag atccgcgagg
     4801 cgttcgtccg ctcgctgatg gcgcgctacc acttccgcgt cgcgtatccg gcgctgccgc
     4861 gtcgcgatca gccgccgccg ccgagcgggc gcgaagcgcg cagcgaggcg acgagcccgt
     4921 cgatcgccgt gatcggtccg gcggcgtcgg cgacgtaagc ggcgggcgga cggctcggtt
     4981 gggtgggggt tttcgagagc cggggacgag ggacgagagc cgggagccga attctgggcg
     5041 ggccgttggg gagattacgc ggacgagaga cgagggacga aattaacctc ggggacggtg
     5101 atcccctgtt tggttccgcc aggcgtcctc tggaatatgg aacaggtgaa agcagggcat
     5161 gatgaaatcg accggttcaa aattctcacg cagccagcgg aacttctcgc cctgttccgg
     5221 cgtgatgccg accagttgat tggtggcgac cagcacccag cccgtcgttt tccccgccac
     5281 ggggtcggtg tcgatttcct gaccgaactc gtcgatgaac cgcttcgccg cgcggcctcc
     5341 ctggccccag tcctggttgc tgtcgatgaa ccagcgccag gccttggccg gcccgcccac
     5401 aatcgggttg aagtaaccca gttgatgggg gcagagcgac agatcgagca ggatgaacca
     5461 cagcacgagc gtgagcccga ccgcgcgcca tgcgcgccgc gaaaacgcca gcacgcccca
     5521 cgcggccgcg gcgaacagaa gcggaaacag cggcagcatg tgccggatgc cgatctggat
     5581 gcccgaaccg aacgtgccat aaagcaggag cgccagcggc ggcagaacca ggaagcaggc
     5641 ggcgcgcagc gagggccctg cgcgctggcg caccaccgcg accagcgcca gcagcagcat
     5701 gccgtggagc gcgaggggcg gcttggccag cagcagcatg gcgaaatacg ggcggaaacc
     5761 ggccgcgtgg cgccactggc cgtgaaaata ggtgtgccgg tactggatcg acatcagcag
     5821 ttcgtccatg ccatgccagt agggccacgg cagcggcgcg gggagccagc ccaggagctt
     5881 gccgacgagt tgcaacaggc cgtggcggac gtgaaagctg gccagcggct gccccaggcc
     5941 gtcgaaatcg tagaccagcg ccgggagcac gatcgccagg cccagcatca gcaggacctg
     6001 ggcggcgacg gcgccgcgtg aggccgcgcc gcgcgcccgc ggcggggcga ggcgccggcc
     6061 gagccagagc gccatgccga cggtcatgcc gcccagccac agcaggccgg tatacttggt
     6121 cagcagggcg atgccgccca gcaggccgac ggccagggcg agccgtcccg attcgcggcg
     6181 cgaccattcg agcgccgccg ccgacagcag gaagcacacc atcgtgacca ggacgtcgtt
     6241 cgtggccagg acggcgtggg gcgtcagcgt gggtgaaaag aggatcagcg cctgggcgag
     6301 cagccccgtc gcgggcgtga accagtggcc cacgatccgg gcgaagagga ggctccagag
     6361 cagaaacagc gccagcgtga cgcagcgagc cgaccgcagc gtccactccc caccgtagcc
     6421 cggcagagga tcgatcggat cggccggcgt cggcatccgc ttgagcagcg cggggatggc
     6481 gaggatcgcg cggccggggg gatggacgat gtcccacgac agattgcgct gacgccagac
     6541 gacgacgccg acgttaacgt ggacgcgctc gtcgtacgtc tcggtgctga acaggagcat
     6601 ccagagcagc cagggggcgg ccgcggccgc cagcaggacg gccagcaacc cgtggcgcgc
     6661 ccgccacggc gccgttccca tagcgtcggg cgacggcatc agaccggggg attcggcacg
     6721 aacgccccgc cgcggcagtg cttcaggtag ttgagggcat ggacctggcc ggtcatttcg
     6781 agttcgccgc gccagaccgc atcgtgccag ccctcgatgt caatcgtgcc gcggtagccg
     6841 gcgcggcgca gttcgctgat gatgtcggtc cagttggagt cgccgaagcc gggggtgcgg
     6901 tggaaggcga acggccgggg gccgtcgacg ccgtattcgc gcacgacgtc ccacatcacg
     6961 gtggcgtcct tgccgtgcag gtgatagatc cggccgaccc atttgcgcag ttggggcagc
     7021 gggtcgatca ggcggaccat ctggtggcag ggttcccact ccaggccgag cgtgtcgccc
     7081 gggacggcgt cgaacatcat ttcccaggcg gtggggttat gggcgatgtt gaaatcgccg
     7141 ctggcccagt cgccgcccat gtcgcagttt tcgaaggcga gcttcaggcc cttgtccgcg
     7201 gcgcgccggg cgagcgggcc gaagacctgc ttaaaccggg ggatggactg gtcgatcggc
     7261 tg
//
LOCUS       DAOPCO010000200         7244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9267_length_7244_cov_5.157602, whole genome shotgun sequence.
ACCESSION   DAOPCO010000200 DAOPCO010000000
VERSION     DAOPCO010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9267_length_7244_cov_5.157602"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="PLS90_12600"
     CDS             complement(<1..1095)
                     /locus_tag="PLS90_12600"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HPK03285.1"
                     /translation="MKQMLTLEESANYNRKILNYIHQASETGVYDIRGLGAKRRVPSL
                     DDLVFLTASLSRYPLEGYREKCVTKTVLGTRFAKKPIELEMPITIAGMSFGSLSANVK
                     EALGRAATHMGISTTTGDGGMTPEERNSSKTLVYQCLPSRYGFNPDDVRKADAIEIVV
                     GQGAKPGGGGMLLGLKVSERVARMRTLPPGIDQRSACRHPDWTGPDDLTIKIQELREI
                     TDWKVPIYIKFGATRVREDVKLAIKAGADVIVVDGMQGGTAATQEVFIEHAGIPTLPA
                     IRLAVEALEEMDMLGKVQLVVSGGIRTGADVAKALALGADAVAIGQGVLMSLGCNTPH
                     YIDADGRPVDVTEEYQALGTAPGYCHHCQTG"
     gene            complement(1092..1781)
                     /locus_tag="PLS90_12605"
     CDS             complement(1092..1781)
                     /locus_tag="PLS90_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HPK03286.1"
                     /translation="MSETFDLKTTTVRAINEHLHGLAPDATGVYEILNPNGSHNIAVG
                     VDAPVEINIRGHAGYFTAGMNKQARVTVYGNVGWSVAENMMSGRVHVKGFASQCAGAS
                     GHGGLLVIDGDASSRCGISMKGIDIVVGGSIGHMSAFMAQAGRLVVLGDAGKGLGDSL
                     YEAVIYVRGKIHGLGADARVEDLSEEDRTQIGELLEQAGLSARPAEFTRVASARTLYH
                     WNPTSPQVSVL"
     gene            complement(1778..2680)
                     /locus_tag="PLS90_12610"
     CDS             complement(1778..2680)
                     /locus_tag="PLS90_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPK03287.1"
                     /translation="MCGIVGLFIKNKTMRDQLGALATPMFVCMGDRGPDSGGLAVFNE
                     PTPESAQRWRINVFARNREYPWEPLIHGLRQTHPATDIVASSNHAILMTDQAPEIVAR
                     WLGEYDPMLIVLSTGRVMDLYKDTGHPRELAERYHFTDLRGTHCVGHTRMATESAVTP
                     AHSHPFVAGNDFCLVHNGSLSNHNSLRRLLERRGIQFETDNDTEAVCRFLQWRMREGD
                     TIEESLARGFEELDGFYTLLMGTPERLVLVRDAFACKPGIIADTPDYVAIGSEFRTLA
                     HLPRIHEANLFEPVPEKIYSWTIA"
     gene            complement(2733..4067)
                     /gene="glnT"
                     /locus_tag="PLS90_12615"
     CDS             complement(2733..4067)
                     /gene="glnT"
                     /locus_tag="PLS90_12615"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HPK03288.1"
                     /translation="MTLEQARSFLKKHNVKYVLAQFVDVHGVAKTKAVPVNHFDMILE
                     DGAGFAGFAVWGLGQEPHDPDFMAIGELDTLTLVPWQPGYARIVCTGHVRGKPWPFDT
                     RHILARQVARLKERGWILNTGLEPEFKLLRHFPDGSIRPADDSDLLDKPCYDYKSLSR
                     SRNIIEKIVEALQAVGIDVYQVDHEDANGQFEINFTYTDAMTSADRYVYFRMAAGEIA
                     RDHGVICSFMPKPFANKTGTGTHMHMSISNKSGKSLFYNKADKRGLGLSELAYHFIGG
                     LMEHAPALAAFCAPAVNSYKRLVVGRSLSGATWAPAYISYGDNNRTTMIRIPFGHVEL
                     RLCDSSMNPYLATAAVIAAGLDGIDRKLDPGEPQNFNHYLLSLEDIKQNGIGVLPQTL
                     KEAIDALDDCELMHKELGTDFVTEYVRLKDMEWVEFQRHVSDWELKRYLEFY"
     gene            complement(4173..4841)
                     /locus_tag="PLS90_12620"
     CDS             complement(4173..4841)
                     /locus_tag="PLS90_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03289.1"
                     /translation="MIARTTPIAHMLAARNPNWTADGERTVVRDFGDPGRERAAVADL
                     ALADLSTRPRLGLKGINLAALLARQGLAVPGKLFAVEPFGQGGLIARLGKEEFLLEAG
                     SAESLLASLREALAREADAFLIEREDACFLLAGERRGAVFAQTCGYNFAEYPGGVVFT
                     RVAGVSCMLLEWPLNGRPAWRIGCAPSYGPYLWEALREITVDCGGMAVGQDALALNVP
                     PSSS"
     gene            complement(4838..>7244)
                     /locus_tag="PLS90_12625"
     CDS             complement(4838..>7244)
                     /locus_tag="PLS90_12625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HPK03290.1"
                     /translation="AGPAGLSAAASAGARVLLVDESPRLGGSLTWHGNLDDSRDRLAD
                     ALRRVEAHQRIQTRTATCVAGLYADGWAALIDRLRLTKLRARAVIVATGVFEQPAVFP
                     HNDLPGVLFAGAAQRLIHHYAVLPAERPVILTANDEGYRAALALRKAGAEVVAVVDTR
                     PEGDSPAAALLKREGINLLAGRFIARARPAGGLAGIRGVELCRVDERGLPRIEQRAEQ
                     LACDAVVMSVGWTPNDTLLRMVGTRMEYSAEREQFLPVELPGGVFAAGKVNGLVGLED
                     RWADGLHAGQAAASALGFGAAPGGSRPAPAGPAINHPYPVSDPDEGKNFVDLDEDLQV
                     KDFRNAIQEGFNSPELLKRYSTVGMGPSQGKHANLMALRVLAKERGEPLAGKQITTAR
                     PFSTPVALGHLAGRIFTPYRHTPAHAFHTERGARMMFAGTWLRPEYYEVVGKTREACI
                     QEEALHVRGSVGLIDVGTLGKMELGGPDAVRFLERIYTGRFDNLKVGASRYGVACDES
                     GVVIDDGVVARLGPNRFYVSTTTSGADAIYREWQRWALCWGLKVILNNATAHWGAMNL
                     AGPRARQVLQPLVDISLAAADFPYMGVRECRLDGVPARVLRVGFVGELGYEIHVPAGH
                     ALGVWNRLMEAGRAADVRPFGVEAQRLLRLEKGHIIVSQDTDGLTQPFEAGLGWAVKM
                     DKPFFIGQRSLKIHQRRPLKRTLVGFQTDEARPRTMPRENHLIIEGKEMIGRVTSIAY
                     SPTLKRTIGLAYLPPSMTAPDTRFTIRADGGELVPARICRIPFYDPAGERQRMTTPEE
                     DAR"
BASE COUNT         1142 a         2395 c         2458 g         1249 t
ORIGIN      
        1 ccccgtctgg caatggtggc aatagccggg ggccgtgccc agcgcctgat actcctcggt
       61 gacgtcgacc ggccggccgt cggcgtcgat gtaatgcggg gtgttgcagc cgagcgacat
      121 cagcacgccc tggccgatgg cgacggcatc ggcccccagc gcgagggcct tggcgacgtc
      181 ggcgccggtg cggatgccgc ccgatacgac caactggacc ttgccgagca tgtccatctc
      241 ttcgagggcc tcgacggcga gacgaatcgc cggcagggtc gggatgccgg cgtgctcgat
      301 gaagacttcc tgggtggccg ccgtgccgcc ctgcatcccg tcgacgacga tgacgtcggc
      361 gccggccttg atcgccagct tgacatcctc gcgcacgcgc gtggcgccga atttaatgta
      421 aatgggcact ttccaatcgg tgatttcgcg caattcctgg atcttgatcg ttagatcgtc
      481 cggtccggtc cagtcgggat gccggcaggc ggagcgctga tcgatccccg gcgggagcgt
      541 ccgcatgcgc gccacgcgct cgctgacctt caggcccagc agcatgccgc cgccgcccgg
      601 cttggcgccc tggccgacga cgatctcaat cgcatcggcc ttgcgcacgt cgtcgggatt
      661 gaagccgtag cgggaaggaa gacactggta gaccagcgtt ttggaggagt tgcgttcctc
      721 gggcgtcatc ccgccgtcgc cggtcgtggt tgagatgccc atgtgagttg ccgcgcggcc
      781 gagcgcttcc ttgacgttgg ccgagagcga accgaaactc atgccggcga tcgtgatcgg
      841 catttccagc tcgatcggtt ttttggcgaa gcgcgtcccc aggaccgttt tggtcacgca
      901 cttttcccga tagccctcga gcgggtagcg cgaaagcgag gccgtgagaa agaccaggtc
      961 atcgagcgac gggacgcggc gtttggcgcc caggccgcgg atgtcgtaga cgccggtttc
     1021 cgaggcctga tggatatagt tcagaatttt ccggttgtag ttggcgctct cttccagcgt
     1081 cagcatctgt ttcatagcac cgatacctgc ggactcgtgg gattccagtg gtagagcgta
     1141 cgggccgagg ccacgcgggt aaactcggcc ggcctggcgc tcaggccggc ctgttccagc
     1201 aattcgccga tctgcgtccg gtcctcctcg ctcagatcct ccacccgcgc atcggcgccg
     1261 aggccgtgga ttttcccgcg cacgtagatc acggcttcat agagcgaatc gccgaggccc
     1321 ttgccggcgt cgcccaggac gaccaggcgg ccggcctgcg ccatgaacgc cgacatgtgg
     1381 ccgatgctgc cgcccacgac gatgtcgatg cccttcatcg agatgccgca gcgcgacgag
     1441 gcatccccgt cgatgacgag cagcccgccg tggcccgagg cgccggcgca ctgcgaggcg
     1501 aaacccttga catggacccg gcccgacatc atgttttcgg ccacgctcca gccgacgttg
     1561 ccgtaaaccg tcacgcgcgc ctgcttgttc atcccggcgg tgaaatagcc ggcgtgcccg
     1621 cggatgttga tctccaccgg cgcgtcgacg ccgacggcga tgttgtggct gccgttggga
     1681 ttgaggatct cgtagacgcc ggtcgcgtcg ggcgccaggc cgtgcaggtg ctcgttaatc
     1741 gcccgcacgg tcgtggtttt caggtcgaaa gtctcgctca tgcgatcgtc catgaataga
     1801 tcttctcggg aaccggttcg aagaggttcg cctcgtggat ccggggcagg tgggccagcg
     1861 tgcggaactc cgagccgatg gcgacatagt ccggggtgtc ggcgatgatg cccggcttgc
     1921 aggcgaacgc atcgcggacc agcaccagcc gctcgggtgt ccccatcagc agggtgtaga
     1981 agccgtcgag ttcctcgaag cccctggcca gcgattcctc gatggtgtcg ccctcgcgca
     2041 tgcgccattg caggaagcgg cagaccgcct cggtgtcatt gtcggtctcg aactggatac
     2101 cgcgccgctc cagcagccgg cgcaggctat tatggttcga cagtgagcca ttgtggacca
     2161 ggcagaagtc attgccggcc acgaacggat gggaatgggc gggggtgacg gccgattcgg
     2221 tcgccatgcg cgtgtgaccg acgcagtggg tcccgcgcag gtcggtgaaa tggtagcgct
     2281 cggccagttc gcgcggatgg cccgtatcct tgtagagatc catgacgcgg cccgtcgaga
     2341 ggacgatgag catcgggtcg tactcgccga gccagcgcgc gacgatctcg ggcgcctgat
     2401 ccgtcatcag gatcgcgtga ttgctgctgg cgacgatgtc ggtcgccgga tgcgtctggc
     2461 gcaggccatg gatcagcggt tcccaggggt actcgcggtt gcgggcgaag acgttaatcc
     2521 gccagcgctg ggcggactcg ggcgtcggct cgttgaagac cgccagtccg cccgaatccg
     2581 ggccgcgatc gcccatgcag acaaacatcg gggtggcgag cgcgccaagc tggtcgcgca
     2641 tggttttgtt tttgatgaag agtccgacaa tgccgcacat ggggttggtt cctttcggta
     2701 atccgcgcgg gggagggatt caacccttca ttttagtaga attccaggta acgcttcaac
     2761 tcccagtccg aaacgtggcg ttggaattcg acccattcca tgtccttcag ccgcacgtat
     2821 tcggtgacga aatccgtccc cagttccttg tgcatcagtt cgcagtcgtc gagggcgtcg
     2881 atggcttcct tgagggtctg gggcaggacg ccgatgccgt tttgcttaat atcctccagc
     2941 gagaggaggt agtggttgaa attctgcggt tcgcccggat cgagtttgcg atcgatgccg
     3001 tcgaggcccg cggcgatgac ggccgccgtc gccaggtacg ggttcatgct cgagtcgcac
     3061 aggcggagtt ccacgtgacc aaacgggatg cggatcatcg tcgtgcggtt gttgtcgccg
     3121 taggagatgt aggccggcgc ccaggtggcg cccgacagcg agcggccgac gaccaggcgc
     3181 ttgtaggagt tgacggccgg cgcgcagaac gccgccagcg ccggggcatg ctccatcagg
     3241 ccgccgatga agtgataggc cagctccgac agccccagcc cccgcttgtc ggccttgttg
     3301 tagaagagcg atttgccgct cttgttgctg atcgacatgt gcatgtgcgt accggtgccg
     3361 gtcttgttgg cgaagggctt gggcatgaag gagcagatga cgccgtggtc gcgcgcgatc
     3421 tcgccggcgg ccatgcggaa gtagacatag cggtcggccg aggtcatcgc gtcggtgtag
     3481 gtgaagttga tttcaaactg gccgttggcg tcctcgtggt cgacctgata gacgtcgatg
     3541 cccacggcct ggagcgcttc gacgatcttc tcgatgatgt tgcggctccg cgagaggctc
     3601 ttgtagtcgt agcacggctt atccagcagg tcgctgtcgt cggcggggcg gatcgagccg
     3661 tccggaaagt ggcgcagcag tttgaactcc ggctcaaggc cggtgttgag gatccagccg
     3721 cgttccttca gccgggcgac ctggcgcgcc aggatgtggc gcgtatcgaa cggccagggt
     3781 ttgccgcgga cgtggccggt gcagacgatc cgcgcgtaac ccggctgcca cggcaccagc
     3841 gtcagcgtgt cgagctcgcc gatggccatg aagtcggggt catgcggctc ctggcccaga
     3901 ccccagacgg caaaacccgc gaagccggcg ccgtcctcca ggatcatgtc gaaatgattg
     3961 acgggaacag ctttcgtctt ggccacgccg tggacatcca cgaactgggc caggacgtac
     4021 ttcacgttgt gcttcttgag gaagctgcgg gcttgctcca gcgtcatgcg cctcgtctcc
     4081 tttgccagtg ttggatcgct acaagcttcg ggttgctgat gaacccgcgg gtttcaacgc
     4141 atctccaagc cgaccggtcc ttcgcgcgcc gatcacgagg acgagggagg gacattcagc
     4201 gcgagggcgt cctgtcccac ggccattcct ccgcaatcga cggtgatctc ccgcaacgcc
     4261 tcccacagat acggcccgta gctcggcgcg cacccgatgc gccaggccgg ccgaccgttc
     4321 agcggccact ccagcagcat gcacgagacg ccggccacgc gggtaaagac gacgccgccg
     4381 gggtattccg cgaagttgta gccgcaggtc tgcgcgaaga ccgcgccgcg gcgctcgccc
     4441 gccagcagga agcaggcatc ctcccgttcg atcaggaacg cgtcggcctc gcgggccaac
     4501 gcctcgcgca gggaggccag gaggctttca gcggaacccg cctcgagcag gaattcctcc
     4561 ttgccgaggc gggcgatcaa gcccccctgg ccgaacggct cgacggcgaa aagcttcccc
     4621 ggcacggcga ggccctgccg cgccagcagt gcggccaggt tgatcccctt caggcccagc
     4681 cgcggccgtg tgctcagatc ggccagcgcc aggtcggcga cggcggcgcg ctcgcgcccc
     4741 ggatcgccaa agtcgcgcac gaccgtccgc tctccgtccg ccgtccagtt tggattccgc
     4801 gcggccagca tgtgggcgat gggggtggtc cgggcgatca tcgcgcgtcc tcctcggggg
     4861 tcgtcatgcg ctggcgttcg cctgcggggt catagaacgg gatgcggcag atccgcgccg
     4921 gcacgagttc cccgccgtcg gcccggatcg tgaaccgcgt gtcgggcgcc gtcatcgagg
     4981 ggggcaggta ggccagcccg atcgtccgct tgagcgtcgg gctataggcg atgctggtaa
     5041 cgcgcccgat catctccttg ccttcgatga tcagatggtt ctcgcgcggc atcgtgcgcg
     5101 ggcgcgcctc gtcggtctgg aaccccacca gggtgcgctt gagcgggcgc cgctggtgga
     5161 tcttcaggct gcgctggccg atgaagaagg gcttatccat cttgaccgcc cagccgagcc
     5221 ccgcctcgaa cggctgggtc aggccgtcgg tatcctggct cacgatgatg tgtccctttt
     5281 ccaggcgcag gaggcgctgc gcctcgacgc cgaaagggcg cacgtcggcg gcgcggccgg
     5341 cttccatcag acggttccat acaccgagcg catggccggc cgggacatga atttcgtagc
     5401 cgagctcgcc gacgaagccc acgcgcagca cgcgcgccgg gacgccgtcc aggcggcact
     5461 cgcgcacacc catgtagggg aaatccgcgg cggcgagcga gatatcgacg agcggctgca
     5521 agacctgacg cgcgcgcggt cccgccaggt tcatcgcccc ccagtgcgcg gtggcgttat
     5581 tgaggatgac cttcaacccc cagcagagcg cccagcgctg ccattcgcgg tagatcgcgt
     5641 cggcgcccga ggtcgtcgtg ctcacataga accgattggg gccgagccgc gcgacgacgc
     5701 cgtcgtcaat cacgacgccg ctctcgtcgc aggcgacgcc gtaacgcgag gcgcccacct
     5761 tcaggttgtc gaagcgcccc gtgtagatcc gctccagaaa ccggaccgcg tcgggtccgc
     5821 cgagttccat cttgccaagc gtgccgacgt cgatcaggcc gacggagccg cgcacgtgca
     5881 gcgcctcctc ctggatgcac gcctcgcggg tcttgcccac cacctcgtaa tactccggcc
     5941 gcagccaggt gccggcgaac atcatgcggg cgccccgctc cgtgtgaaac gcatgcgccg
     6001 gcgtatggcg atacggagtg aaaatgcggc cggcgaggtg gcccagcgcg acgggggtcg
     6061 agaagggccg cgccgtcgtg atctgcttgc ccgccagcgg ctcgccgcgt tccttcgcca
     6121 ggacgcgcag cgccatcagg ttggcgtgct tgccctggct gggccccatc ccgacggttg
     6181 aataacgctt gagcagctcc ggactgttga agccttcctg gatggcgttt ctgaagtcct
     6241 tgacttgcag atcctcatcc agatcaacga agttcttccc ctcgtccggg tcgctcaccg
     6301 ggtagggatg attgatcgcc ggaccggccg gcgcgggacg cgacccgccc ggggcggcgc
     6361 cgaagccgag ggccgacgcc gcggcctgac cggcatggag cccgtcggcc cagcggtcct
     6421 ccaggccaac gagtccgttc acctttccgg ccgcgaacac gcccccgggc agttcgacgg
     6481 ggaggaactg ctcgcgctcg gcgctgtatt ccatgcgcgt tccgaccatc cgcagcagcg
     6541 tgtcgttcgg cgtccagccg acgctcatga ccacggcgtc gcaggccagt tgttcggcgc
     6601 gctgctcgat ccgcggcagc ccgcgctcgt cgacccggca cagctccacg ccgcggatgc
     6661 cggcgaggcc gcccgccggc cgcgcgcggg cgatgaagcg cccggcgagc aggttgatcc
     6721 cctcgcgctt gaggagggcc gcggcgggcg agtcgccttc ggggcgggta tcgacgaccg
     6781 ccacgacctc cgcccccgcc ttccgcaggg cgagcgcggc gcggtagccc tcgtcgttcg
     6841 ccgtcaagat gaccggccgc tcggccggca gcaccgcata gtggtggatc aaccgctgcg
     6901 ccgcgccggc gaacaacaca ccgggcaagt cgttatgcgg gaagacggcg ggctgctcga
     6961 agacgccggt ggcgacgatc accgcccgcg cccggagttt cgtcagccgg agcctgtcga
     7021 tcagcgccgc ccagccgtcg gcatacagac cggcgacgca ggtggccgtc cgcgtctgga
     7081 tgcgttggtg ggcttcaacc cggcgcagcg cgtcggccaa ccggtcccgc gagtcgtcga
     7141 ggttcccatg ccaggtgagc gaaccgccca ggcgcgggga ttcatccacg agcagcacgc
     7201 gggccccggc gctggccgcg gccgacagcc cggccggacc ggcg
//
LOCUS       DAOPCO010000201         7196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9327_length_7196_cov_4.311721, whole genome shotgun sequence.
ACCESSION   DAOPCO010000201 DAOPCO010000000
VERSION     DAOPCO010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9327_length_7196_cov_4.311721"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLS90_12630"
     CDS             complement(<1..241)
                     /locus_tag="PLS90_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03291.1"
                     /translation="MQLSCPHCQHVWDDVPPERATQVMCPECLELAPVLRAGASAAAS
                     AAAAPPRQAAPAAAAGGGATIPQSDFGSIPGSRPPA"
     gene            622..1176
                     /gene="hslV"
                     /locus_tag="PLS90_12635"
     CDS             622..1176
                     /gene="hslV"
                     /locus_tag="PLS90_12635"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPK03292.1"
                     /translation="MHAPEPTGATFHGTTIVAVRKDGKTCVGGDGQVTFNHQILKAGA
                     RKVRRLHGGQVLVGFAGATADAMTLFEKLEAKLQEHGGNLQRAAVELAKEWRTDRYLR
                     RLEAMLIAADQTSLFLISGNGDVLEPDSSVIAIGSGGGYALAAARALMDRAELDAEAI
                     VRRAMEIAAELCIYTNDRIVVETL"
     gene            1197..1862
                     /locus_tag="PLS90_12640"
     CDS             1197..1862
                     /locus_tag="PLS90_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPK03293.1"
                     /translation="MTPPAPDRCLIKICGVTRPEDGLFALESGADWLGFIRWPKSARY
                     RPAEDCAAALAAIRRAAPRPFQAVGVYVNADPAVIAEEGAAIGFDRFQLHGEETPEYA
                     RALARPVIKVLRIRDAASLAEAERYAGLDLLTDTWDPALPGGTGRGYDYTLLARLVAR
                     RRVIVSGGLTPANVGEVVRAVGPWGVDVASGVESAPGIKERAKVLAFISAVRDAEGQS
                     APC"
     gene            1878..2900
                     /locus_tag="PLS90_12645"
     CDS             1878..2900
                     /locus_tag="PLS90_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03294.1"
                     /translation="MLTRLPALLIPLIFALALPAAAQQAPRPVIPTEGLPQAGQQAPA
                     VRTRAPGNANAPAATATPAPAATAAPAAAAAAQQAVVPPTTAELDKIVITRVKSYRKN
                     GAEGARAEAVSPTGSGRLFRSPGDPGMEVQFRLLPPQGWSIVSVDDAQLVRALDQAGN
                     PLTVTPNPKPAANATESRFNMVSPAQELSERPIVSLRAQEPARGEELLQQIVATFQIT
                     LGQTVRQQYKNLRAYTGKQLPNLPVPGLTLIVEEMTNEEVVLRALGKVDHLGSLQLVG
                     ANGGEVVPYAQEEVSNINENAGQIQRTWRLSFAGLPEMVHLNMTAYPVMTTQTVQFEQ
                     AYVPLP"
     gene            2976..3335
                     /locus_tag="PLS90_12650"
     CDS             2976..3335
                     /locus_tag="PLS90_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPK03295.1"
                     /translation="MIVRSLAGARPFTTKDGSTIRSLLDRSNAPVMNQSLAEATLPPG
                     GATETHYHQDSEEFYYVVQGEGRMRIGEEERAVTVGDAILIPPGAVHTLRNTGTGELK
                     ILCCCAPPYAHEDTYFP"
     gene            complement(3380..4951)
                     /locus_tag="PLS90_12655"
     CDS             complement(3380..4951)
                     /locus_tag="PLS90_12655"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPK03296.1"
                     /translation="MTQQPAPQRVVIFDTTLRDGEQSPGFSMNLQEKLELAHQLARLG
                     VDVIEAGFPITSPGDFEAVHAIAGQVHGPVICALARAVEKDILRAGEALAPAARKRIH
                     TFIATSNIHVEKKLRMSREQVIETAFNAVKLARSFTDDVEFSCEDAGRTDWDYIVAVL
                     TAAIEAGATTLNVPDTVGYTVPEQFGQCIRYVREHTPGIENCIISVHCHNDLGHAVAN
                     SLAGVRNGARQIECTINGIGERAGNTSLEEAVMMLHVRKDYWGLETGIRTEEIYRTSR
                     LLSRITGVRVQPNKAIVGANAFAHEAGIHQDGVIKERTTYEIMHPADVGWLGEGMVMG
                     KHSGRAALAARLEQLGIKGLSPEDITAAYERFKKLCDQKKTVYDEDLLAIVEDQVSLQ
                     RAIYRLVDLEASTSMRGGPRVRVAIAVGDAGEQHEALCTEGDGQVDALYRALDQLTPH
                     TIKLLEYGIESVTEGKDALGRVMVKMNIDGDEVRGTGVATDVIVASAHAYLNALNRFE
                     MLKEISERAASTATP"
     gene            complement(5199..5729)
                     /locus_tag="PLS90_12660"
     CDS             complement(5199..5729)
                     /locus_tag="PLS90_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03297.1"
                     /translation="MRPHRCPALAGVLILLLALACGGSNEPVASRESEQWLEVQVVAG
                     PTERSRDGVITIPRGGIKPEVLTSGIVKITPEGLPQQVLGPDGNIPIQVTWLRSASLP
                     KGPGVELEHLRVQIPGPVRVNEAEFLTDVTLEPEQPVLSILPEYSAVGLVYDNGSGSS
                     NVYVRARWLAKSPAAG"
     gene            6473..>7196
                     /locus_tag="PLS90_12665"
     CDS             6473..>7196
                     /locus_tag="PLS90_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPK03298.1"
                     /translation="MAEPKLTPPAGQKITVNQGRLTVPDHPIIPQIIGDGIGPDISQA
                     MVTVIDAAVNKAYGGGRAIAWFPVPAGEEAWEKYENYLPEATLEAIREYHVAIKGPLS
                     TPVGGGIRSLNVALRQIFDLYACVRPVRWFQGVPSPVKHPEKLNVVIYRENTEDIYKG
                     IEWPEGSDEARKMIRILKEEFGKEVREDSGIGIKPVSVTGSRRLVKKAIEHAIEKQLP
                     SVTLVHKGNIMKFTEGAFREWGY"
BASE COUNT         1193 a         2200 c         2682 g         1121 t
ORIGIN      
        1 tcgcgggcgg ccgggagccg gggatcgagc caaagtccga ttgcgggatc gtcgcgcccc
       61 cccccgcggc ggcggcgggc gcggcctggc gtggcggcgc ggcggcggcg gaggctgcgg
      121 cggaggcgcc cgcgcggaga accggcgcca gctccaggca ctcggggcac atcacctggg
      181 tcgcgcgttc gggcggcacg tcgtcccaga cgtgttggca gtgggggcag ctcagttgca
      241 ccggttggga cctccctctt acgatgcagc gccggccgat ggcgtggatt ggcggcgctc
      301 agggctccag atttcgcggg cgggttccct ggaaggctgt tctcgtgcct atcaatgtag
      361 gccggaatcg ccgccgcgtc aaaatggaac gtcccggccg ggacgcgcgc aggacgcccg
      421 gggcgggcgg tcggggggtg aatctctcgt tgcgggggcc agggggctaa cctagagtgg
      481 agacaaagcg cggcggcgcg tgtattccgc gagccagcac ccgacgactc cgcgagggcc
      541 gacgccggag cacgccggag cacccgggga tcaacgccgg accgccccgc gcgacgcaac
      601 caccgcaggg ggaagcccgc catgcacgcg cccgaaccga ccggcgccac gtttcacggc
      661 acgacgatcg tggccgtccg caaggacggc aagacctgcg tggggggcga cggccaggtg
      721 acgttcaacc accagatttt gaaggcgggg gcgcgcaagg tccgccggct gcacggaggc
      781 caggtgctgg tgggttttgc cggcgcgacg gccgacgcga tgacgctgtt cgagaaactc
      841 gaggccaaac tgcaggagca cggcggcaac ctgcagcgcg cggcggtgga gctggccaag
      901 gagtggcgca ccgaccgata cctgcggcgg ctggaggcga tgctgatcgc ggcggaccag
      961 acgagtctgt ttctgatctc gggcaacggc gacgtgctgg aacccgattc gagcgtgatc
     1021 gcgatcgggt cgggcggagg gtatgcgctg gcggcggcgc gcgcgctgat ggaccgggcg
     1081 gagctggatg ccgaggcgat cgtccggcgc gcgatggaga tcgccgccga gctgtgcatc
     1141 tacaccaacg accgcatcgt cgttgaaacg ctttgagccg ggccgcttcg ctgccgatga
     1201 cgccccccgc tcccgaccga tgcctgatca agatctgcgg cgtgacgcgg ccggaggacg
     1261 ggctgttcgc gctggagtcg ggcgcggact ggctcggatt catccgctgg ccgaagtcgg
     1321 cgcggtaccg accggcggag gactgcgcgg cggcgctcgc cgccatccgg cgggccgccc
     1381 cgcggccgtt tcaggccgtc ggcgtctatg tgaacgcgga cccggcggtg attgccgagg
     1441 aaggtgcggc gatcggtttc gaccgatttc agcttcacgg ggaggaaacg ccggagtatg
     1501 cccgggcgct ggcgcgtccg gtgatcaagg tgctgcggat ccgcgatgcc gcgtcgctgg
     1561 ccgaagccga acgctacgcc gggctggacc tgctgaccga cacatgggat ccggccctcc
     1621 cgggggggac gggccgcggc tacgattaca cgctgctggc gcggctggtt gcgcgccggc
     1681 gggtgatcgt gtcgggggga ctgacgccgg cgaatgtggg cgaagtggtg cgcgcggtcg
     1741 gtccgtgggg ggtggatgtg gccagcgggg tggagtcggc gcccgggatc aaggagcggg
     1801 cgaaagtgct ggcgttcatt tcggccgtac gcgacgctga agggcaatcc gccccgtgct
     1861 aaggagactt cgccgccatg ttgacgcgcc ttcccgccct gctgatcccg ttgattttcg
     1921 cgctggcact gcccgccgcg gcgcagcagg cgccccggcc ggtgatcccc accgagggtc
     1981 tgccgcaggc cgggcagcag gcgccggcgg tgcggacgcg tgcgccgggc aacgccaatg
     2041 cgccggcggc gaccgcgacg cccgccccgg cggccaccgc cgcaccggcc gcggccgccg
     2101 cggcacaaca ggccgtcgtg ccgccaacca cggccgagct ggacaagatc gtcatcacgc
     2161 gcgtcaagtc ctaccgtaag aacggggcgg agggggcgcg cgccgaggcg gtcagcccga
     2221 ccggctcggg ccggctgttc cgatcgccgg gcgatcccgg catggaggtg cagttccggc
     2281 tgctgccgcc gcaggggtgg tcgatcgtga gcgtggacga tgcgcagctt gtgcgagcgc
     2341 tggaccaggc gggcaatccg ctgacggtga cgccgaaccc gaaaccggcg gccaacgcga
     2401 cggaatcgcg gttcaacatg gtcagcccgg cgcaggaatt gagcgagcgg ccgatcgttt
     2461 cgctgcgcgc gcaggagccg gcccggggcg aggaactcct gcaacagatc gtcgcgacgt
     2521 tccagatcac gctggggcag accgtccggc agcaatacaa gaacctgcgg gcctacaccg
     2581 gcaagcagtt gccgaatctc ccggtgccgg gcttgacgtt gattgtggaa gagatgacga
     2641 acgaggaagt cgttctgcgc gcgctgggaa aggtcgatca tctcggcagc ctgcagttgg
     2701 tcggcgccaa cggcggggaa gtggtgccct acgcccagga agaagtgagc aacatcaacg
     2761 agaacgccgg acagatccag cgcacgtggc gtctgtcgtt cgccggcctg ccggagatgg
     2821 tgcacctgaa catgacggcg tatccggtga tgacgacgca gacggtgcaa tttgagcagg
     2881 cgtatgtgcc gctgccgtga gccaacaagg cattggagag gcgaggacga gggacggaat
     2941 ttggagtaag tgacagggca ggggatattg aggggatgat cgtacgctcg ctagccgggg
     3001 cccggccgtt cacgacgaag gatggctcga cgatccggag tctgctggat cggtccaatg
     3061 cgccggtgat gaaccagtcg ctggccgagg cgacgctgcc gccgggcggc gccaccgaga
     3121 cgcactatca tcaagattcc gaggaattct attacgtcgt gcagggcgaa ggccggatgc
     3181 gcatcggcga ggaggaacgc gccgtgacgg tcggcgacgc gattctgatc ccgccggggg
     3241 cggtccacac gctgcgcaac acggggaccg gagagttgaa gatcctgtgc tgctgcgcgc
     3301 cgccctatgc ccatgaggac acgtattttc cctgaagcgc ccggcggcgc gggggcggcg
     3361 ccaccgggcc gggtcaggtt cagggggtgg ccgtgctggc cgcccgctcg ctgatttcct
     3421 tgagcatttc gaagcgattg agggcgttga gatacgcgtg ggccgaggcc acgatcacgt
     3481 cggtcgcgac gccggtgccg cgcacctcgt cgccgtcgat gttcatcttg accatcacgc
     3541 ggccgagggc gtccttcccc tcggtcaccg attcaatgcc gtattccagc aacttgatcg
     3601 tgtggggggt gagctggtcg agcgcgcgat agagggcgtc gacctggccg tcgccctcgg
     3661 tgcagagggc ttcgtgttgc tcgccggcgt cgccgacggc gatcgcgacg cggacgcgcg
     3721 gcccgccgcg catggaggtg ctcgcctcca gatcgaccag acggtagatc gcgcgctgga
     3781 gcgagacctg gtcctcgacg atcgcaagga gatcctcgtc gtagacggtt ttcttctgat
     3841 cgcagagttt cttgaagcgc tcgtaggcgg cggtgatgtc ctcgggggag aggcccttga
     3901 tgccgagctg ttccagccgg gcggcaagcg cggcgcgccc cgagtgcttg cccatgacca
     3961 tcccttcgcc gagccagccg acatcggcgg ggtgcatgat ctcgtaggtc gtccgctcct
     4021 tgataacgcc gtcctgatgg atgccggctt cgtgggcgaa ggcgttggcg ccgacgatgg
     4081 ccttgttggg ctggacgcgg acgccggtga tccggctgag caggcgcgag gtgcggtaga
     4141 tttcctcggt gcggatgccg gtctcgaggc cccagtagtc cttgcggaca tggagcatca
     4201 tcaccgcttc ctcgagggag gtgttgccgg cccgctcgcc gatgccgttg atcgtgcact
     4261 cgatctggcg cgcgccgttg cgcacgccgg ccagcgaatt ggcgacggcg tgcccgaggt
     4321 cgttgtggca gtggaccgag atgatgcagt tttcgatgcc cggggtgtgt tcgcgaacgt
     4381 agcgaatgca ctggccgaac tgttcgggga cggtgtagcc aacggtgtcg gggacgttga
     4441 gggtcgtggc gccggcctcg atggcggcgg tcaggacggc cacgatgtag tcccagtcgg
     4501 tgcggccggc gtcctcgcaa ctgaattcga catcgtcggt gaacgagcgc gcgagtttga
     4561 cggcgttgaa ggcggtctcg atcacctgct cacggctcat ccggagcttc ttttcgacgt
     4621 ggatgttgct ggtggcgatg aaggtgtgga tgcgcttgcg ggcggcgggg gcgagcgcct
     4681 cgccggcgcg gaggatgtcc ttttcgacgg cgcgggccag ggcgcagatg accgggccgt
     4741 gaacctggcc ggcgattgcg tggacggcct cgaaatcgcc gggcgaggtg atggggaatc
     4801 cggcctcgat cacgtcgacg ccgaggcggg ccaactggtg cgccagctcc agcttctcct
     4861 gcaagttcat gctgaagccg ggcgattgct cgccgtcgcg caacgtcgtg tcgaaaatga
     4921 cgacgcgctg aggtgcgggt tgctgagtca tggtcctgtc ctttggattc aaaaccgatc
     4981 gcgggaacct ggagcccgga ccccgccgcg gcgggtcggg cgcgaacagc aagggtgatg
     5041 gggcttagcg tgattgcgcc gcgaggcgca gtcgcaggga gatcgtaaag gcgcaggcca
     5101 gcgcttcgaa gcgcggggtg gagatggaga tggtgacggt catgttgggg ggcgggagcg
     5161 gggccgaaga ttgtgcgacg cgccgatccc ttcctttctc aacccgccgc cggcgacttg
     5221 gccagccagc gggcacggac gtagacattt gagctgcccg aaccattgtc gtagaccaga
     5281 ccgacggctg aatactcggg caggatactc agcacgggct gttcgggctc cagcgtcacg
     5341 tcggtcagga actccgcttc attgacccgg acggggccgg ggatctgcac ccggagatgc
     5401 tccagttcca cgccgggccc cttggggagc gaggcggagc gcaaccaggt gacctggatc
     5461 gggatgttgc cgtcggggcc cagcacctgc tggggcaagc cctcgggggt gatcttgacg
     5521 atgccgctgg tgaggacctc cggcttgatc ccgccgcggg ggatggtgat cacgccgtcg
     5581 cgggaccgct cggtcgggcc ggcgacgacc tggacctcca gccattgctc ggactcgcgg
     5641 gatgcgacgg gctcgttcga accgccgcaa gccaaggcga gcaacaggat gaggactccc
     5701 gccagcgcgg ggcagcgatg cggacgcatt caacgcttcc cactttcgac ccaaaataaa
     5761 aacgcccccg gaccggaaag acggtccagg ggcgaaaaag ttcgcggtac caccctgttt
     5821 tgatgacggc gccgcggggc ggtccgccat ccttcattcg acgacccggt aacgggggtc
     5881 ggccggcgcg aactacgcat cgcccgcgcc atgacggcga tgggcgactt cccccgcgcg
     5941 gctccgaggc cagttcagac gcggcgccca ctgcctcgca cccaccggca gctctctcca
     6001 ggcgcgctcg acgccctact cttcctcatc ccagccattg tgttctgtcg atagaaccat
     6061 gcgacggagt ccggggaccc cccgcgaaac cacttttata tcacatctat cggtcaagtc
     6121 aagggggttg atgaggatca ttgcggcgcg gggggagcgg cgcggccgtt gcggatagag
     6181 gttgctcatg caggccggca agcaagccgg cggtcccggg cgggtggttg ctcgcagcga
     6241 acggcgtgac ttcgagagcc gaggacgagg gacgcaagac gggagtcaaa tatttgggcg
     6301 gcccgttggg agcgaggacg agggacgagc gaatagggtt gggcggttgg ttgacagggg
     6361 tgggggggta tgccaaatta cggggacgtg tgccatggcc tcccccgcgg tgggcgagcc
     6421 gcgccccccg gcccaatcgt ggcatggcga aatcagacaa caggaagaca tcatggccga
     6481 gccgaagctg acgccccccg cgggccagaa aatcacggtg aatcagggcc gcctgacggt
     6541 gcccgaccat ccgatcatcc cgcagatcat cggcgacggg attggaccgg acatctcgca
     6601 ggcgatggtc acggtgatcg acgcggccgt caacaaggcc tatggcggcg gccgggcgat
     6661 cgcctggttt ccggtgccgg cgggcgagga ggcatgggag aaatacgaga actacctgcc
     6721 cgaggcgacg ctggaagcga tccgcgaata ccatgtcgcg atcaagggcc cgctgagcac
     6781 gccggtcggc gggggtatcc ggtcgctgaa tgtcgcgctg cggcagatct tcgacctgta
     6841 cgcctgcgtc cggcccgtcc gctggttcca gggggttccc tcgccggtga agcatcccga
     6901 gaaactgaac gtcgtgatct atcgggagaa caccgaggac atctacaagg gaatcgagtg
     6961 gccggagggc tcggacgagg cgcgcaagat gatccgaatc ctgaaggagg aattcggcaa
     7021 ggaggttcgc gaggattcgg ggatcgggat caagccagtg tcggtcacgg ggtcgcgccg
     7081 gctggtgaag aaggcgatcg agcatgcgat tgagaaacag ctgccgtcgg tgacgctggt
     7141 ccacaagggc aacatcatga agttcaccga aggggcattc cgcgaatggg ggtatg
//
LOCUS       DAOPCO010000202         7189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9333_length_7189_cov_5.571909, whole genome shotgun sequence.
ACCESSION   DAOPCO010000202 DAOPCO010000000
VERSION     DAOPCO010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7189
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9333_length_7189_cov_5.571909"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2872)
                     /locus_tag="PLS90_12670"
     CDS             complement(<1..2872)
                     /locus_tag="PLS90_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK03299.1"
                     /translation="MNSLTRIIAFIILIAGVHAAETPVRESDRNGMLWPQFRHDSGLT
                     ATSPLTGGLAQAPEELWRFDLGGTLQPAETVRLEDLNGDGHVELLRVLSDRLICQDLR
                     GATLWEVTGLAHPAVTDVRDWTGNGVLGVRVLCNDGVKLEHYMVNGATGASVLLFTQQ
                     DIFGGYERAGHILRDVPGQQLCVWWDGEDPENRNFNGDNMRGDGYLWSFENGPDQPTQ
                     RFHARATGTVYAGQHLFADADGDGQDEFVMIAHQQMWLFNPETGEQKGYLQWQPQIRS
                     YRSLLALAPLNEGEPPAQLEINYMIPGVQVIQQDGHRATRLWKAVVAPKEDQYQVAVK
                     VGAAAPDPFIDMDGDGVPEILATVTNEHGDGQVCLVIFDARTGNRLYDQPIPVSAEQA
                     GIPTGQGPNGNILTVDDLDGDGRLEFLFQEGDLLKIANWNGSGFVERWSGAGVAPLLQ
                     PAPPEGRLDRCTLVGYNCPLWRETADSSAFLLRFPDGVFSCELARDGGVEQLRPVTTH
                     DALGNGPSARQILDSYTWDGRTLVAREDDREVWRYEVPLRRVYSSPSAIVADLAGRTR
                     ILTRSSTGTLLSLAGDGGDPRALLEHHPASIGVDCSSLSGIEPPLVADLEGDGQNEVI
                     ATATVDGTPCTLIMDGSGRVKRRIDPIPTTTQILLGVTGRLGPGQGRWLVIRYTRQAE
                     REAVVAYNGTTGETLWMRDSYTDAAEPYKFVLWLPTSVWDADGDGADELISYSANHYG
                     LIRIRDGSDLFPLVKITAAFPGHWPAYGTPVLADFTGADRPDIFLSRSYSMVCGATLE
                     GAPIWHYGFRRSEGPNRHGVAADLDGDGRDEVVYAQQDGLVRAFSGVPHDATCPACPP
                     GVAPAPANRAGEPLWSMQLRAPVSDMAAADLDGDGRLEVLLGAGDGKLYALEHDGDAG
                     RVLWSVALSDQPVGGPILADLTGTGQPSILVPTDDGYLHCLSA"
     gene            complement(3062..6271)
                     /locus_tag="PLS90_12675"
     CDS             complement(3062..6271)
                     /locus_tag="PLS90_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03300.1"
                     /translation="MVSLPRFTSRLLLSMLFLFLPVARLFAVEHLEDLPGTVILAQSQ
                     AAGYEAVKAIDDSLATEWLSVLSGLPLGSPSWLELQWSAPVSIGSVLLSHSPFYPLRD
                     FTIETWNGEAWVVHDRVTDNLGSSTAHTQNLMSDGNFPDSTTRLRVNIQAGNVYNYPR
                     LPEITVLPIPEQGDGAPLPPETLWPMFRYDPARTGRYPLPGSMPGVPTVKWRYQYGPE
                     PGPIAHHNVDADPALESIHYSAGGVLVCKKADGSVVWESEPIGASVGFLGARDMDAHG
                     DLELLFSYKTSNALETGFALLRPADGMTLWRHTYPPVYGYDYKIGDIDPTRPGLEILF
                     WCHTEGVQGIGEGVYTVFDQGAGNAETLWHQVYNDYMYFPQPIAGDVDLDGKNECIVL
                     THSKIRAYHLDSGLEKWSLRWDWVRSYGAFLQRNLDSDPYPEYIVLSDTSRRVEVIDN
                     TGAGFELLWEAILEYPRDIQPSLMKNTLRYGFTSVADLDGNGRMEVVVNLLEESVDQR
                     WHTLVLDELDGSRVLDLPDTYCHGAADLNGDGRAELFLAAAPNGSDALPAALTVGNWS
                     RSGGFAPLWQSPTPQRYVTLLHDFANDMQNNSIGGTIADVQIFNQAGKSYFKTDAGNW
                     FWEDGAIVGGGKGDPSVAEPKGFALTQYYADKHSPIVADLNADGIAEVLVQDLTQTFQ
                     VVRPPAAAGAAPQQIGSLARGGNVHFWGSWPHFADLDGDGAVEHFRITRRADGGETFH
                     VIDSRNQTVWSREFPPATGSQASQAGIVSYNTGRFNNDPCLDVFVSWYGDAAGEYSLA
                     LSGADGSQLWRLDLLAGQTYQKASTFASGFTAVRDVNRDGYDDIAILAHGVSMGIVNG
                     NTKQTLAMKETTGTNQNDAGHYLYPGKWAVGASPRLVEETPGGALKLLMDDSQDGFGV
                     SDMTLKEDWSIAVGVPYQLRLHFGVGDVDGDGRKEVGIPYSDGLFRCHDLATGEVKWT
                     YDIGSSCSDVITADVDGRGRDEFLFGTGDGRLIALGGEGDAPRLLWSRQFSQEVGSPI
                     MADLDQDGDGEILVTVKDGFLYVLDAADSAAGGHWSLHR"
     gene            complement(6423..>7189)
                     /locus_tag="PLS90_12680"
     CDS             complement(6423..>7189)
                     /locus_tag="PLS90_12680"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK03301.1"
                     /translation="TTETAPHPGYDLLSRHAVPVTFFGKRAPRRFNHIDIDCYGLATE
                     TLASCAARYPIISLITRHNGVVESYFSSEFERGFCDFMRQAGVNNPERLIFTLPERHE
                     CVELGRLAAEQLVARHGLKQAIFTANDLIAEGVMSFVVENNLRFSSEFALISLSDVPF
                     SPYGPQAFSSASLPVVPAGEILAELLIQQLEAGSDWIPRRRLYFPIVVTRHIEKLDPE
                     VRRPLNRQQALKSQGIYEETLLELPSSTHPGQLAAH"
BASE COUNT         1257 a         2302 c         2402 g         1228 t
ORIGIN      
        1 tcgcgctcag gcaatgcagg tagccgtcgt cggtcgggac gaggatcgac ggctggcccg
       61 tcccggtcag atcggccagg atcggcccgc ccacgggctg atcgctcagc gcgacgctcc
      121 acagcacccg tccggcgtcg ccatcgtgct ccagcgcata cagcttccca tcgccggcgc
      181 cgagcagcac ctccagccgc ccgtcgccgt ccaggtcggc ggcggccatg tcgctgaccg
      241 gcgcccgcag ttgcatggac cacagcggtt cgcccgcccg gttcgccggc gcgggggcaa
      301 cacccggcgg gcaggcgggg cacgtggcat catggggaac gccgctgaag gcgcgcacca
      361 ggccatcctg ttgcgcgtag acgacctcgt cccggccgtc gccgtccaga tcggccgcca
      421 cgccgtggcg gttgggcccc tcgctgcgcc ggaagccata atgccagatc ggcgcgccct
      481 cgagggttgc gccgcagacc atcgaatacg accggctcag gaagatatcg ggccgatccg
      541 caccggtgaa atcggccagc accggcgtgc cgtaggcggg ccagtgcccc gggaacgccg
      601 cggtgatctt gacgagcggg aacagatcgc tgccgtcgcg gatgcggatc aacccgtagt
      661 gattggccga atagctgatc agttcgtcgg cgccgtcgcc atcggcgtcc cacaccgacg
      721 tcggcaacca caggacgaac ttgtaaggtt ccgcggcgtc ggtgtagctg tcgcgcatcc
      781 acaaggtctc gcccgtcgtg ccgttgtagg ccacgaccgc ctcgcgctcg gcctggcgcg
      841 tgtagcggat caccagccac cgcccctggc cggggccgag ccggccggtg acgccgagca
      901 ggatctgggt cgtggttggg atcgggtcga tccgccgttt cacgcggccc gatccatcca
      961 tgatcagcgt gcagggagtg ccatccaccg tcgcggtcgc gatgacttca ttttggccgt
     1021 cgccctccag gtcggccacc aggggcggtt cgatcccgga caggctgctg caatcaacgc
     1081 cgatgctggc cggatggtgc tcgagcagcg cgcgcgggtc gccgccgtcg ccggcgagcg
     1141 agagcagcgt accggtgctg gagcgggtca ggatccgcgt ccggccggcc aggtcggcga
     1201 cgatcgccga cggcgagctg tagacgcggc gcagcggcac ttcgtagcgc cacacttcgc
     1261 ggtcgtcctc gcgcgcgacg agtgtccggc catcccaggt gtaactgtcg aggatctgcc
     1321 tggccgaggg cccgttgccc agcgcgtcgt gggtcgtcac cggccgcagc tgctccacgc
     1381 cgccgtcgcg cgcaagctcg caactgaaga ccccgtcggg gaagcgcaag agaaacgcgg
     1441 acgaatccgc cgtctcgcgc cacagcgggc agttatagcc gaccagcgtg cagcgatcga
     1501 gccggccctc gggcggcgcc ggttgcagca gcggcgcgac gcccgcgccc gaccagcgtt
     1561 cgacaaaccc gctcccgttc cagttggcga tcttgagcaa atcgccctcc tgaaacagga
     1621 actccagccg cccgtcgccg tccagatcgt ccacggtcag gatgttgcca ttgggcccct
     1681 ggccggtcgg aatgcccgcc tgctctgcgc tgaccgggat cggctgatca tacaaccggt
     1741 tgccggtccg cgcgtcgaag atgaccaggc agacctgccc gtcgccgtgc tcattggtca
     1801 ccgtggccag gatttccggc acgccgtcgc cgtccatgtc gatgaaggga tcgggcgcgg
     1861 ccgcgccgac cttgaccgcc acctgatact ggtcctcctt cggggccacc accgccttcc
     1921 acagccgcgt ggcgcgatgg ccgtcctgct ggatcacctg cacgccggga atcatgtaat
     1981 tgatctcaag ttgcgcgggc ggttcccctt cgttcagcgg cgccagcgcg aggagcgacc
     2041 ggtagctgcg aatctgcggc tgccactgca gatatccctt ctgctcgccc gtctcgggat
     2101 tgaacagcca catctgctga tgggcgatca tcacgaattc atcctggcca tcgccgtcgg
     2161 cgtcggcgaa caggtgctgg ccggcataga cggtgccggt ggcacgggcg tggaaccgct
     2221 gggtcggctg gtccggcccg ttttcgaagc tccacaggta gccgtcgccg cgcatgttat
     2281 cgccgttgaa attgcggttc tccgggtctt cgccgtccca ccagacgcac agttgctgcc
     2341 ccggcacgtc gcgcagaata tggccggccc gttcgtagcc gccgaagata tcctgctgcg
     2401 tgaacaggag cacgctggcg cccgttgctc cgttgaccat atagtgctcc agcttcaccc
     2461 cgtcgttgca caggacgcgc acgcccagca cgccgttgcc ggtccagtcg cgcacgtcgg
     2521 tgacggcggg atgggccagg ccggtcactt cccacagtgt ggcgccgcgc agatcctgac
     2581 agatgagccg gtcgctcagg acgcggagca actccacgtg accgtcgccg ttgaggtctt
     2641 ccaggcggac ggtttcggcc ggctgcaacg ttccacccag atcgaagcgc cagagttctt
     2701 cgggcgcctg ggccaggccg ccggtgagcg gggaggtcgc cgtcaggccg ctgtcgtggc
     2761 ggaactgcgg ccagagcatc ccgttccggt ccgactcgcg aaccggggtt tcggcggcat
     2821 ggacgccggc gatcaaaatg atgaaagcaa tgattcgggt caggctgttc atggggcacc
     2881 tcagacaaag attgatggca aagcgcgtct acttccacgg gaagggatgg ggatccttgc
     2941 cggattccgc ctggactgct cttcgatggg gtctgttcac ctgccgctgt tgtgcaggga
     3001 ttcaggtgac atcgaaagcc aaccggcaaa ggccgcaagg gtccggaaga aacggcggag
     3061 ttcagcgatg cagcgaccag tgtccgccgg ccgcggaatc agccgcatcc aggacgtaga
     3121 ggaagccgtc cttcaccgtc acgagaatct cgccgtcacc atcctgatcg aggtcggcca
     3181 tgatcggcga gccgacttcc tgactgaact ggcgcgacca gagcagccgc ggggcatcgc
     3241 cttcgccgcc cagcgcgatc aggcggccat cgcccgtgcc gaagaggaac tcatcgcgac
     3301 cgcgaccgtc gacgtcggcg gtgatgacgt cgctgcagct actgccgatg tcataggtcc
     3361 acttgacctc acccgttgcc aggtcatggc agcggaagag cccatccgag taagggatgc
     3421 cgacctcctt gcggccgtcg ccatccacgt cgccgacccc gaagtgaagg cgaagctgat
     3481 aggggacccc gaccgcgatc gaccaatcct ccttcaaagt catgtcgctg acgccgaagc
     3541 catcctgcga gtcatccatc agcagtttga gtgccccccc gggcgtctcc tcgaccaggc
     3601 gcgggctggc gccgacagcc cacttgcccg gatagagata gtgccccgca tcgttctgat
     3661 tcgttcccgt cgtttccttc atggcgagcg tctgctttgt gttgccattg acaattccca
     3721 tgctgacgcc atgggccagg atcgcgatgt cgtcgtagcc gtcgcggttg acgtcgcgga
     3781 cggcggtaaa cccggaagcg aacgtggacg ccttctgata ggtctggccc gcgagcaggt
     3841 cgaggcgcca gagctgcgag ccgtcggcgc cactgagggc aaggctgtat tcaccggcgg
     3901 catcgccata ccaactgacg aacacgtcca ggcagggatc gttgttgaag cgaccggtgt
     3961 tgtagctgac gatccccgcc tgcgacgcct gcgaacccgt agccggcggg aattcgcggc
     4021 tccagacggt ttgattgcgg gaatcgatga catggaaggt ttcgccgcca tcggcccggc
     4081 gcgttatccg gaaatgctcc acggcgccgt cgccgtccag atcggcgaag tgcggccagg
     4141 agccccagaa gtggacgtta ccgccgcggg cgagcgatcc gatctgctgc ggggccgcgc
     4201 cggcggcagc gggaggccgc acgacctgga atgtctgcgt gagatcctgc accaggactt
     4261 cggcaatccc atccgcgttc agatccgcca cgatcggcga atgcttgtcc gcgtagtact
     4321 gcgtgagggc gaaacccttg ggttcggcta cggaaggatc gcccttgccg ccgccgacga
     4381 tcgcgccgtc ctcccagaac cagtttccgg cgtcggtctt gaaatatgac ttgccggcct
     4441 ggttgaaaat ctggacatcc gcgatcgttc caccgatcga gttgttctgc atgtcgttgg
     4501 cgaagtcgtg gagcagcgtc acgtagcgct ggggcgtggg cgactgccag agcggggcaa
     4561 agccgcccga ccggctccag ttgccgacgg tgagggcggc gggcagcgca tcgcttccat
     4621 tgggcgcggc ggcaaggaaa agctcggcgc ggccgtcgcc gttcagatcg gcggcgccgt
     4681 ggcagtaggt atccggaaga tcgagcacgc ggctcccatc gagttcatcc agcacgagcg
     4741 tgtgccagcg ctggtcgacc gattcctcca gcaggttcac taccacttcc atgcgcccat
     4801 tgccatccag gtcggccacg gaagtaaagc cgtagcgcag cgtgttcttc atcagggaag
     4861 gctggatatc gcgggggtac tccaggattg cttcccagag caattcgaag ccggcgccgg
     4921 tgttgtcgat cacctccacc cgccggctgg tgtcgctcag cacgatgtac tccggatagg
     4981 gatccgaatc cagattgcgc tggagaaagg cgccgtaact gcgaacccag tcccaacgga
     5041 gcgaccattt ttcgaggccg ctgtcgaggt gataggcacg gatcttggag tgcgtcagga
     5101 cgatgcattc gttcttgccg tcgagatcga catcgcccgc gatgggctgg gggaaataca
     5161 tgtaatcatt gtagacctga tgccagagcg tctcggcgtt gccggcgccc tgatcgaaaa
     5221 cggtgtagac gccctcgccg atgccctgga cgccttcggt gtggcaccag aagagaatct
     5281 ccagccccgg acgcgtcgga tcgatatcgc cgatcttgta atcgtagccg tagaccggcg
     5341 gataggtgtg gcgccagagc gtcatgccat cggccggacg cagcagggcg aaacccgttt
     5401 ccaacgcatt gctggtcttg tagctgaaga gcagttcaag atcgccgtgg gcatccatgt
     5461 cgcgggcgcc gaggaaaccg accgacgcgc caatcggttc ggactcccaa acgaccgacc
     5521 catcggcctt tttgcagacc agcacgccgc cggccgagta atggatcgac tcgagcgccg
     5581 gatcggcatc gacgttgtga tgcgcaatcg ggcccggttc ggggccgtat tgatagcgcc
     5641 acttcaccgt cggcaccccc ggcatgctgc ccggcagggg gtaacgcccc gtgcgggccg
     5701 gatcgtagcg gaacatcggc caaagggttt cgggcggcag gggcgcgccg tcgccctgct
     5761 cggggatcgg caggacggta atttccggca ggcgcgggta attgtacacg ttgcccgcct
     5821 ggatattgac ccgcaggcgc gtggtggagt cggggaaatt gccgtccgac atcaggttct
     5881 gcgtgtgagc ggtgctggag ccgaggttgt cggtcacccg gtcgtgaacg acccacgcct
     5941 cgccgttcca ggtctcgatc gtgaaatccc gcagcgggta gaacgggctg tggctcagga
     6001 ggaccgagcc gatggagacg ggtgcgctcc attgcagctc aagccagctg gggctgccca
     6061 ggggtagacc gctcaggacg ctgagccact cggtggcgag gctgtcgtcg atggctttga
     6121 cggcttcgta gccggcggcc tggctttgag cgaggatcac ggtccccggc agatcctcca
     6181 ggtgctccac ggcgaagagc cgagcaacgg ggagaaaaag gaagagcatg ctgaggagaa
     6241 ggcgcgaggt gaagcgaggc aaggagacca tcacgaagag ccgtcaaggg gtaaatgtgg
     6301 gggtcgaacg tttgaatcat cgcacggttc accctgccaa tcaacccctg ttccgcgatc
     6361 cgcgccaagg ttcctgtgcg aaccctggag tggagtggtg acgatgtgtc cctgcctgat
     6421 gctcaatgcg cggcgagttg cccagggtgg gtcgaagacg ggagttccag caatgtttcc
     6481 tcgtagattc cctggctctt gagcgcctgc tgccgattca aggggcggcg aacctcgggg
     6541 tcgagtttct caatatggcg tgtcaccaca atcgggaaat agaggcgccg gcgggggatc
     6601 cagtcgctgc ccgcttccag ctgctggatc agcagttcgg ccagaatctc gccggcaggc
     6661 acgaccggca acgacgccga cgaaaaagcc tgtggcccgt aaggcgagaa cgggacatcg
     6721 ctcaggctga tcagggcgaa ctcgctggag aagcggagat tgttctcgac cacgaagctc
     6781 atgacccctt cggcgatgag atcattggcg gtgaagatcg cctgcttgag gccgtgccgc
     6841 gcgacgagtt gctcggcggc aaggcggccc agttcgacac actcgtggcg ctcggggagc
     6901 gtgaagatga ggcgctcggg gttgttgaca ccggcctggc gcatgaaatc gcaaaagccg
     6961 cgctcgaact cggagctgaa gtacgattcg acgacgccgt tgtggcgagt gatcaggctg
     7021 ataatcgggt atctggcggc acaggaggca agcgtctccg tcgcgaggcc gtagcaatcg
     7081 atatcgatat gattgaatcg ccggggagcc cgcttgccga aaaaggtaac cggcacggcg
     7141 tgacgggaga ggaggtcgta tcccggatgc ggcgccgtct cggtggtcg
//
LOCUS       DAOPCO010000203         7093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9504_length_7093_cov_4.635976, whole genome shotgun sequence.
ACCESSION   DAOPCO010000203 DAOPCO010000000
VERSION     DAOPCO010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7093
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_7093_cov_4.635976"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLS90_12685"
     CDS             <1..308
                     /locus_tag="PLS90_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPK03302.1"
                     /translation="DPTLGWGAACLAVFIAYVRAEGKVAGAHSEFCGPMAKPHRMAAV
                     TVGALYLALAPAAWQPVQAATGWGVPALVLALILLGGVVTVYRRLARITAVLKGKPL"
     gene            305..1309
                     /locus_tag="PLS90_12690"
     CDS             305..1309
                     /locus_tag="PLS90_12690"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPK03303.1"
                     /translation="MNDAVHDRLFDSARAFDSPVTVGLTLAVAAALVGGLLIVELLGR
                     GGRIGAAQFDELRKRMISWCVLAPAMILPVLLGAAWTILAVGLLGLLCYREFALATGF
                     FRHRLLSFIVALGIVLLTFAALDHWYHFFLALPSLTIVTLAAFAILPDQPRGYLQRVA
                     LGTFGYLFFGVCLLHLAYMANDPGYRPIILMLLIAVELNDVFAYIAGKSFGRRKLCPA
                     TSPGKTVGGSVGAFACTTLLVALLGAIVFHGTPVAHPLRLIGLGLLVSVAGQLGDLML
                     SSIKRDLGIKDMGVVIPGHGGLLDRFDSLILVAPAAFHYLAYLDGLRLSPLTRILSGV
                     "
     gene            1314..2075
                     /locus_tag="PLS90_12695"
     CDS             1314..2075
                     /locus_tag="PLS90_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK03304.1"
                     /translation="MDEWRYEPARDLGLTPRDSFRSVRRERGLLSLAASLAWMLITRA
                     WLRLYHRLEVRGREHLPAAPPFLVVANHSSHLDAPALAAALPLHLALRAFPVAAGDTF
                     FESRATAAFASLMMNALPIWRRNCGPHALGELRQRLLGEPCVFILFPEGTRSRTGAMG
                     AFKPGIGRLVCGAPTPVPVVPCHLDGAFAALPPHARYPRPRKLTLRIGPPIVMDDLPD
                     RREGWERITHAAEAAVRALAPQTSNVVPRPSSSSS"
     gene            2308..2886
                     /locus_tag="PLS90_12700"
     CDS             2308..2886
                     /locus_tag="PLS90_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPK03305.1"
                     /translation="MHRSVFSPRGLALWVVGAILLAAPLRAQSIKIGIVDMELVTNRS
                     ASIQNRMKSAESSMQSQKAELDEKYTRLQRLRRDLRDQRSVLSESQISEKEAQIRDLD
                     EEVNDLQYEINKQIERVRTDVIEPELNRIEATIERVAREGQYDLILVKDAVLFSSEKV
                     DITPLIIQALDRESGRAPQLPESGEIESENEE"
     gene            2883..3737
                     /gene="lpxC"
                     /locus_tag="PLS90_12705"
     CDS             2883..3737
                     /gene="lpxC"
                     /locus_tag="PLS90_12705"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPK03306.1"
                     /translation="MTGFAPRQQTLAGPVETRGIALHHGVDVAVRLVPAPPDHGIVFE
                     RVDLPGRPRIAVSPESVDAGGLQRRTELAGSGAARVATPEHLLAACAGLGVDNLLVEL
                     GGPELPIFDGSAAPWVEWIDRAGLAEQAAPRRCFRLRRAVTLMEGDADLVALPAERPH
                     FTFFATLRHAGLPNQAAVFDPDRDDFRRALAPARTFCFYDEVRHLMDNGLIKGGSFDC
                     ALVLRDGAPINAQYRLPNELAAHKLLDLIGDFAILGRPVAAHVSARGSGHALHYAFIR
                     KLQEELIA"
     gene            3734..4192
                     /gene="fabZ"
                     /locus_tag="PLS90_12710"
     CDS             3734..4192
                     /gene="fabZ"
                     /locus_tag="PLS90_12710"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPK03307.1"
                     /translation="MTGSPLTLPVDAVAIRKILPHRYPFLLVDRVIELVPNESITALK
                     NLTQNEPFFAGHFPEYPVLPGVLQIEMIAQTGAIMLLTSVEEAAGKIAMLTGADEFKF
                     RRPILPGDVATIHVTDFKVRRTFGKALGRVSVADEVCAEGMVSFALVATE"
     gene            complement(4217..5512)
                     /gene="murA"
                     /locus_tag="PLS90_12715"
     CDS             complement(4217..5512)
                     /gene="murA"
                     /locus_tag="PLS90_12715"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPK03308.1"
                     /translation="MDRFLIQGNRRLSGEVHVGGAKNAALPLMVACLLADGTSTLCNV
                     PDLRDIQTMAGVLGELGARIDYEDHRMRIDATGFSNHEAPYDMVRKMRASTYVMGPML
                     ARLKRARVSFPGGCAIGARPIDLHLRGFEALGAKIQIEGGYVVADGANMKGAEMTLEG
                     PHGSSVGATINVLMAAVLTPGRSVIRGAALEPEVSEVVRMLNLMGARIEGADSPTLTV
                     EGVEALRPIYYSTIPDRIEAGTFLLAGAVTRGDVLVRGARADHMNALLDKMIQAGCEL
                     EVLPDAIRVRGDRGPFRATSIRTLPYPGFPTDMQAQFMTLLCLAEGESLVSESVFPER
                     FIHAAELMRMGAKIEMGRGEARVKGVKKLTGAPVMASDLRASAALVLAGLAAEGETEI
                     LRVYHIDRGYERIDEQLAGVGAMIWRESAPTGSLGKVPS"
     gene            complement(5930..6373)
                     /locus_tag="PLS90_12720"
     CDS             complement(5930..6373)
                     /locus_tag="PLS90_12720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK03309.1"
                     /translation="MPVTFLEFTPQDYDEALNFWRATPGIGLSETDSRENIEAFIQRN
                     PGLSFLARDAGRLVGTVLGGHDGRRGYLYQLAVLPAYRGKGVGTELVERCITRLGEIG
                     IQKCHIMIYATNIQGQTFWESIGWHRRTEILVMSRLTSAAPSDDV"
     gene            complement(6419..6826)
                     /locus_tag="PLS90_12725"
     CDS             complement(6419..6826)
                     /locus_tag="PLS90_12725"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HPK03310.1"
                     /translation="MRGRLVAAAWLALAAALAASIYLRPPGEAWYPSCAFHALTGLHC
                     SGCGTVRALHALLHGHFAQAWAWNALTVSLLPFLAGWGVVQLWRAWSGRPAWGGRIPA
                     WTIWTLLAIIVAFWILRNLPFHPFPLLAPHRLL"
BASE COUNT         1055 a         2709 c         2084 g         1245 t
ORIGIN      
        1 cggaccccac gctgggctgg ggcgccgcct gccttgccgt gttcattgcg tatgtccgcg
       61 ccgagggcaa agtcgccggc gcccattcgg aattctgcgg accgatggcc aagccgcacc
      121 gcatggccgc ggtcacggtc ggcgcgctgt atctggcgct tgcccccgcc gcctggcagc
      181 cggtccaggc cgccacgggc tggggcgttc ccgccctcgt gctggccctg atcctcctcg
      241 gcggcgttgt caccgtgtat cgccggctgg cgcgcatcac cgccgttttg aaaggcaagc
      301 ccctttgaac gacgccgtcc atgatcgcct gttcgattcg gcccgcgcct tcgacagccc
      361 tgtcacggtc ggcctgaccc ttgccgttgc ggcggccctt gtcggcgggc ttctcatcgt
      421 cgaattgctc ggccgcggcg ggcggatcgg cgccgcccag ttcgacgaac tgcgcaagcg
      481 catgatctcg tggtgcgtgc tggcccccgc catgatcctg ccggtgctgc tgggcgccgc
      541 ctggacgatc ctcgccgtcg gcctgctcgg cctgctctgc taccgcgagt tcgccctcgc
      601 caccggcttc ttccgtcatc ggctgctcag tttcatcgtc gcgctcggca tcgtgctgct
      661 tacgttcgcc gccctcgatc actggtatca tttcttcctg gcgctgccgt cgcttacgat
      721 cgtcaccctc gccgccttcg ccattctgcc cgaccagccg cgcggctatc tgcagcgcgt
      781 tgccctgggc acgttcggct acctcttctt cggcgtctgt ctgctgcacc tggcctacat
      841 ggccaacgat cccggctacc gccccatcat cctgatgctg ctgatcgctg tggaattgaa
      901 cgacgtcttc gcctacatcg cgggcaaatc cttcggccgg cgcaagctct gccccgccac
      961 cagtccgggc aagacggtcg gcggctcggt cggcgcgttc gcctgtacca cgctgctggt
     1021 cgccttgctc ggcgccatcg tttttcacgg cacgcccgtt gcccatccgc tgcgcctgat
     1081 cggcctcggg ctgctcgtca gcgtggccgg ccaactcggc gacctgatgc tctcctcgat
     1141 caagcgcgac ctgggcatca aggatatggg cgtggtcatc cccggccatg gcggcctgct
     1201 cgaccgtttc gacagcctga tccttgtcgc ccccgccgcc tttcattacc tggcctacct
     1261 ggatgggctg cgcctgtcgc cgctgacgcg catcctcagc ggagtctgaa ccggtggacg
     1321 agtggcgcta cgaaccggcg cgcgacctcg ggctgacccc gcgcgacagc tttcgcagtg
     1381 tccgccgcga gcggggcctc ctctcgttgg ctgcctcgct ggcctggatg ctgatcacac
     1441 gcgcctggct gcgcctctac caccggctcg aggtccgcgg gcgcgagcac ctgcccgcgg
     1501 cgccgccgtt ccttgtcgtc gccaatcact ccagtcacct ggacgccccc gcgctcgccg
     1561 ccgcgctgcc gctccatctt gccctgcgcg cgttcccggt ggccgccggc gatacgttct
     1621 ttgagtcgcg cgcgacggcc gcctttgcct ccctgatgat gaacgccctg cccatctggc
     1681 gccgcaactg cggtccgcac gccctcggcg agttacgcca gcgcctcctc ggcgaaccgt
     1741 gcgtcttcat tctcttcccc gaggggacgc gcagccgtac gggcgccatg ggagccttca
     1801 agccgggcat cggtcggctc gtctgcggcg cgcccacgcc cgttccggtc gtgccgtgcc
     1861 atctcgacgg cgccttcgcc gcgttgccgc cccacgccag atatcccagg ccgcgcaagc
     1921 tgacgcttcg catcggccct cccatcgtca tggacgacct gcccgacagg cgcgaaggct
     1981 gggagcgcat cacgcacgcc gccgaagccg ccgtccgcgc cctcgccccc cagacgtcta
     2041 acgtcgtccc ccgtccctcg tcctcatcct cgtgatcgcc ccaaaggcct gcagattcgt
     2101 cgatggtgca tgagcagttc gtgaccgcct gtcttcacgc gtcaccaagg ggcttcagcg
     2161 acagaagggc ggtcctggcg cgcggcctcg cccccttgtc ccgctcccgc gaaactgcta
     2221 gtctgttgcc cggtccgccg tcgcgccgcc ttcatccggc gcatgcggcg accccactta
     2281 tcttcccgaa agtcaggttc tcacgtcatg caccggtccg tcttctcccc tcgcggtctc
     2341 gcactctggg tcgtcggcgc gatcctgctg gccgcccccc tgcgcgccca gtcgatcaag
     2401 atcggcatcg tcgacatgga gctggtcacc aaccgcagcg ccagcattca gaaccggatg
     2461 aaatcggccg aaagctcgat gcaatcccag aaggccgagc tggacgagaa gtatacccgc
     2521 cttcagcgcc tgcgccgcga cctgcgcgac cagcgctccg tcctctccga gtcccagatc
     2581 agtgagaagg aagcgcagat ccgcgacctg gacgaagagg tcaacgacct gcaatacgag
     2641 atcaacaagc agatcgagcg cgtccgcacc gacgtgatcg aaccggagct caatcgcatc
     2701 gaagcgacga tcgagcgcgt cgcccgcgaa ggccagtatg atctgatcct ggtcaaggat
     2761 gccgtcctgt tctcctcgga gaaagtggac atcacccccc tcattattca ggccctcgat
     2821 cgggagtcgg gccgcgcgcc gcagctcccc gagagcggcg agatcgagag cgaaaacgag
     2881 gaatgaccgg attcgccccg cgccagcaga ccctggccgg ccctgtcgag acccgcggca
     2941 tcgcgctgca ccacggtgtc gacgtggccg tccgcctggt cccggcgcct cccgaccacg
     3001 gcatcgtctt cgagcgcgtc gatcttcccg gccggccgcg catcgccgtc tcgcccgaat
     3061 cggtcgatgc cggcggtctc cagcgccgga ccgaactggc cggctccggc gcggcccgcg
     3121 tcgccacgcc cgagcatctg ctcgccgcct gcgccggact gggggtcgat aatcttctcg
     3181 tcgaactggg cggccccgag ctgccgatct tcgacggcag cgccgcaccc tgggttgaat
     3241 ggattgaccg cgccggtctc gccgagcagg ctgccccgcg ccgctgtttc cgcctgcgcc
     3301 gcgccgtgac gctaatggaa ggcgacgccg acttggtggc cctccccgcc gagcgccccc
     3361 atttcacctt cttcgccacg ctgcgccatg ccgggctgcc gaaccaggcg gccgtcttcg
     3421 accctgaccg cgacgatttc cgccgcgctc tcgctcccgc tcgcaccttc tgcttctacg
     3481 acgaagtgcg tcacctgatg gacaacggcc tcatcaaggg gggatcgttc gactgcgctc
     3541 tcgtcctgcg cgacggcgcg ccgatcaacg cccagtaccg cctgcccaac gaactggccg
     3601 cccataaact gctcgacttg atcggcgatt ttgccatact ggggcggccc gtcgccgccc
     3661 atgtcagcgc gcgcggctcc ggacacgccc tgcactacgc cttcatccgc aaactccagg
     3721 aggaactgat cgcatgaccg gctcccccct cacgcttccc gtcgacgccg tcgccatccg
     3781 caagatcctg ccccaccgct accccttcct gctggtcgac cgcgtcatcg agctggtgcc
     3841 caacgagtcg atcaccgccc tgaagaacct cacccagaac gagccgttct tcgccggcca
     3901 tttcccggaa taccccgtcc tgcccggcgt gctgcagatc gagatgattg ctcagaccgg
     3961 ggccatcatg ctgctgacga gcgtggagga agcggccggc aagatcgcca tgctgaccgg
     4021 cgccgacgaa ttcaagttcc gccgcccgat cctgccgggc gatgtcgcca cgattcacgt
     4081 caccgatttc aaggtccgcc ggacattcgg caaggccctc ggccgcgtct cggttgccga
     4141 cgaggtctgc gccgaaggca tggtcagctt cgcgctcgtc gccactgaat aaccccccct
     4201 gcgccagcgc gcccgcctac gacggcactt tccccagcga ccccgtcggc gcgctctcgc
     4261 gccagatcat cgcgcccacg cccgccagtt gttcgtcgat ccgctcgtag ccccggtcga
     4321 tgtgatacac gcgcaggatc tcggtctccc cctcggccgc caggccggcc agcaccagcg
     4381 ccgccgacgc tcgcagatcc gacgccatca ccggcgctcc ggtcagtttc ttcacgccct
     4441 tgacgcgcgc ctcgccccgg cccatctcga tcttcgcccc catgcgcatc agctccgcgg
     4501 cgtggatgaa ccgctcgggg aagacgctct cgctcaccag gctctctccc tcggccaggc
     4561 acagcagcgt catgaactgc gcctgcatgt cggtcgggaa gccggggtag gggagcgtcc
     4621 ggatcgacgt cgcgcggaac gggcccctgt cgccccgcac gcggatcgcg tcgggcagca
     4681 cctccagttc gcagccggcc tggatcatct tgtccagcag cgcgttcatg tgatcggccc
     4741 gcgcgccgcg caccagcacg tcgccgcgcg tcaccgcccc cgccagcagg aacgtccccg
     4801 cctcgatccg gtccgggatc gtcgagtaat agatcggccg cagcgcttcg accccctcca
     4861 ccgtcagcgt cgggctgtcg gccccctcga tgcgcgcccc catcaggttc agcatccgca
     4921 ccacttcgct cacctcgggc tccagcgccg ctccgcggat cacgctgcgc cccggcgtca
     4981 gcacggccgc catcagcacg ttgatcgtcg ccccgacgct gcttccatgc gggccttcca
     5041 gcgtcatttc ggcgcccttc atgttggcgc cgtcggcgac cacgtagccc ccctcgatct
     5101 ggatcttcgc tcccagcgcc tcgaacccgc gcaggtggag atcgatcggc cgggcgccga
     5161 tcgcgcaccc gccggggaag ctcacccgtg cccgtttcag ccgcgccagc atcgggccca
     5221 tcacgtaggt cgaggcgcgc atcttgcgca ccatgtcgta cggcgcctcg tggttgctga
     5281 aaccggtcgc gtcgatccgc atccggtggt cttcgtagtc aatccgggcg cccagttcgc
     5341 ccagcacgcc ggccatcgtc tggatgtcgc gcaggtcggg cacgttgcac agggtggatg
     5401 tgccgtcggc cagcaggcag gcgaccatca gcggcagcgc ggcgttcttc gccccgccga
     5461 cgtgaacttc gcccgaaagg cggcggttgc cttgtatcag aaatcgatcc atgggagcgg
     5521 tccaaaccga gtcgaaactg catcaccggc gccaacgccc ggctggcttc catcatccac
     5581 accggcccgg cccgtggcga ggtcggtttt cgagccgtcc agttgcctga agtgacttgc
     5641 gagatgggaa gggcgcgtct ggcttgatgg cgcgggcgtg tcaagcgaat gcttaccccg
     5701 ccgccagggg cgggtgggtg gaagaggaac cttcgtattg gttaagaaaa atccgtcttg
     5761 agtcgtcacc gggccgcggc agcctcggcc cggtgaccgg gcaacaacta ttcttcaacc
     5821 gtttcaacgg ctttcacgcc aagcccgaca agccgatgac cgcatcgccc cggccatcgc
     5881 ccctttccgt gcctttcgtg tcttccgtga tccggaattt ctttcgcgct tacacatcgt
     5941 ccgacggcgc ggcgctcgtc agtcgcgaca tcaccagaat ctccgtccgc cgatgccacc
     6001 cgatcgattc ccagaacgtc tggccctgga tgttcgtcgc gtagatcatg atgtggcatt
     6061 tctggatgcc gatctcgccc agccgcgtga tgcagcgctc gaccagctcc gtccccaccc
     6121 ccttgccccg gtaggccggc agcaccgcca gttgatacag gtagccgcgc cggccgtcgt
     6181 ggcccccgag caccgtcccc accagccgcc cggcgtcgcg cgccagaaag ctcagccccg
     6241 ggttgcgctg gatgaacgct tcgatatttt ctcgcgagtc ggtctcgctc aggccgatcc
     6301 ccggcgtggc acgccagaag ttgagcgcct catcatagtc ctgcggtgtg aattcaagaa
     6361 acgtaacggg catggcacgc ggctcctgtc gggcgtgtga ctcggctcca ttttacggtc
     6421 agagaagccg atgcggcgca agcagcggga acggatggaa cgggagattg cgcaaaatcc
     6481 agaaggcgac gatgatcgcc aacagcgtcc agattgtcca tgccggaatg cgcccgcccc
     6541 atgccggccg ccccgaccat gccctccaga gctgaaccac cccccagccc gccaggaacg
     6601 gcagcaggct caccgtcaac gcgttccagg cccacgcctg ggcgaagtgg ccatgcagca
     6661 gcgcatgcag cgcccgcacc gtcccacacc cgctgcaatg caggcccgtc agtgcatgga
     6721 acgcgcagct cggataccag gcctctcccg gcgggcgcag atagatcgac gccgccagcg
     6781 ccgccgccag cgccagccag gccgcggcga cgagcctgcc ccgcattctc acccggctcg
     6841 ccatcggcgc gacactgaca cgctcactcg aacccatcaa atcggcaacc actttggaaa
     6901 accctcgctc caagtgcagc gaatcccggg gaatacatcc ccatcacgcc cgagcaattt
     6961 ccggaactct tcgggtgcca ttctcccatc ctgggcgatt ccccctggtc aacgcatgaa
     7021 tcgggttatg ccgcggaatc acaaggattc gcatcacaac atgtttgcgc tacccgcccc
     7081 cgccgcgttc act
//
LOCUS       DAOPCO010000204         7071 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9537_length_7071_cov_5.085376, whole genome shotgun sequence.
ACCESSION   DAOPCO010000204 DAOPCO010000000
VERSION     DAOPCO010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9537_length_7071_cov_5.085376"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..990
                     /gene="tatC"
                     /locus_tag="PLS90_12730"
     CDS             85..990
                     /gene="tatC"
                     /locus_tag="PLS90_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPK03311.1"
                     /translation="MSLIGHLTELRTRLIVCLLGLAAGVFLAFFIADPVLSLLTRPIK
                     GMAREPDRTAALELHLAPDGTLSARAPGDLSQVSMRHFVLVVEDDPLTTATEGGRLLI
                     GDPPTQRFYYHSPLDPIMIKLKVAMVVGVLLALPLILWQVWLFISPGLTAGERRVAKP
                     ILMGAVVLFPIGAVFAYGVIALLLRVMQHYAPPDIDPLLNIFDYLRVMTTMMLVFGVV
                     FELPLIVAIAARVGLVTPAWLQTYRRHAYVVLAVAAMVLTPADPYSMLIALVPLIGLY
                     ELSVALARPMALLHERDARTAAFVD"
     gene            1080..1676
                     /locus_tag="PLS90_12735"
     CDS             1080..1676
                     /locus_tag="PLS90_12735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03312.1"
                     /translation="MQALVKFIAIAILATFGLTGCSFHLFGPPKDTFYETVLDRFPDN
                     RAALFGQGQVLLRNGQYQEALPYLKRLTRLEPDNQQVWLTLGRAYLEARDGLRAERAF
                     RRAIGIRPTMDGELGLGNALMLQGDLSAARSVAERIAVTYGESAYLWRLRGDIAFMAD
                     DPAEAARAYNESLGLDPAQPSVQERLADLSALVQVSQH"
     gene            1734..2522
                     /locus_tag="PLS90_12740"
     CDS             1734..2522
                     /locus_tag="PLS90_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPK03313.1"
                     /translation="MTQTIAISNQKGGVGKTTTAVNLAACLADLGHRTLLVDLDPQSN
                     ASSALGFDSNRLDASIYDALLDHAAPTVHPLDAQHSNLFLLPSSMDLAGAEFELIDRE
                     GRESALRDALGRLDQPFDYILIDSPPSLGLLTLNALVAARWVLIPVQAEYLALEGLSR
                     MVQTIQRINRKQNPELEILGLLATLYDSRTNLAHQVLEELRRAFPDKVFSTPVTRSVR
                     LSEAPSYGQTILRYDARSSGALQYQQIAQEVIHVCEKASLGSRA"
     gene            2506..3390
                     /locus_tag="PLS90_12745"
     CDS             2506..3390
                     /locus_tag="PLS90_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPK03314.1"
                     /translation="MGRGLDALLGADSAPTAVATVPDVPASAIVMIDVSRITSNPYQP
                     RRHFAPEALEELAASIEQNCILQPLVVRRAAAAGQFELVAGERRWRAAQRLALAEVPA
                     IVREYTDREMLELALIENLQREELNAVEEARAYRRLIDEFGLTQEQVAERVGKSRVAV
                     TNSLRLLRLSDRLLGWLDDGSLSAGHARALLGLDNEGLQLALAREIMTKELSVRETER
                     RVRTLNQAPNPQPKAQTPTVDIDTADLEEKLTLHLGLQVKIHPRTNTTGRIEVFYSSL
                     DEFSRVFDHLGISLEQEL"
     gene            3395..4258
                     /locus_tag="PLS90_12750"
     CDS             3395..4258
                     /locus_tag="PLS90_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPK03315.1"
                     /translation="MKARFGSHPSGRDLDYLPPASRYRIALMFLLFLVGTATIALAQT
                     APNSNLQNWIGVDPIAMLYDSDIIGQACLIILAGFSIISWMVIVYKYLHIRQASTQTD
                     AFVEVCNQGSGELEEAFRLASNFPDSPLAQILREGYLELEIENWYRDGYDLDPQARLD
                     IIKVSVERVFERTITSEINHLESKLVFLATTSSVCPFIGLFGTVWGIMGTFQTAMSGS
                     QAVALSSLAPGISTALLTTVGGLFCAIPASVMYNYLTHRVRMLISRMDAFALELGNVV
                     QKQVMKQGAMV"
     gene            4280..4756
                     /locus_tag="PLS90_12755"
     CDS             4280..4756
                     /locus_tag="PLS90_12755"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPK03316.1"
                     /translation="MRRHRRDLEVLSDMNLTNLLDTAFILLMAFMLVAPMLKTGIDLN
                     LPRVGAGQLQGQQKTVTISIAKPQVEGQSERIYVDDARVTYEELEEKLAETKSNYQAF
                     SVAIEADAEATYQSVARVLSLLKAMQITSVGLVTDPAASNQQAGSAAAPEPEDQSE"
     gene            4763..5689
                     /locus_tag="PLS90_12760"
     CDS             4763..5689
                     /locus_tag="PLS90_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016645854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPK03317.1"
                     /translation="MTRSLFVSIFVHCLLVVGLLVLSRLAPPVERMESLTVELISAMP
                     LPAEPLPVATPEPDIEPTPEPTPRPTPEPTPEPTPEPTPEPTPEPTPEPTPEPTPEIV
                     IPKPTETPTPTPTPTPKPTPKATPKPTPTPAPKKSRDPKEKLDDAKVLEEEPEPTPKP
                     TPKATPKPPPPPVPVITPEPEPAPAIPSLPSTDEPVRRVDAKAGESVSIQASGEGNLA
                     MTYLVQVRQRIEANFSHPFSNTGRRATVEFTIQRDGTISRDFKIIQSAGPSMDHYAIQ
                     ALERTKDLPPLYDSYTKPFLRCQITFEFNQKN"
     gene            5801..6769
                     /locus_tag="PLS90_12765"
     CDS             5801..6769
                     /locus_tag="PLS90_12765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPK03318.1"
                     /translation="MALSAFDFEQPVIDLENELKALQELNPPPDNLAEQVSALRERIE
                     RTRREIYDNLSAWQILQIARHQARPRALDYVRRIFTDWTELHGDRAYGDDHACIAGFA
                     LLDEQPVCLIGQQKGLDVRDNAYRNYGMMHPEGYRKALRVMRLAEKFGRPVICLLDTA
                     GAFPGVGAEERGQAEAIARNILEMSTLRTPVICIVIGEGGSGGALGIGVGNRLLMQQY
                     AFYSLISPEGCASILWRDAKFAPEAARALKFTARDLKELGVIDEIIPEPEGGAHRDAN
                     MAARLVGQAIRGHLAQLRAMSIDEIVEDRFQKFRRIGQWEEIETAP"
BASE COUNT         1263 a         2572 c         2031 g         1205 t
ORIGIN      
        1 gccccgctgc ccggccccgc ttctctaccc tgacccctcc ccgttgccca ggcccttggg
       61 atggattcaa accctgatcg cgaaatgtcc ctcatcggac atctgactga gttgcgcacg
      121 cgactcatcg tctgtctgct ggggctcgcc gccggcgttt tcctcgcctt cttcattgcc
      181 gacccggtcc tcagcctgct gacacgcccc atcaagggga tggcccgcga acccgatcgc
      241 accgccgcgt tggaacttca tctcgccccc gacggtacgt tgtccgcgcg cgcgcccggc
      301 gatctctctc aggtctccat gcggcacttc gtcctcgtgg tggaggacga tcccctcacc
      361 accgcaaccg aaggtggacg cctgctcatc ggcgaccccc ccacccagcg gttctactac
      421 cacagtccgc tcgatccgat catgatcaag ctcaaggtcg ccatggtcgt cggcgtcctg
      481 ctcgccctgc cgctgattct ctggcaggtg tggctcttca tctcgccggg gctcacggcg
      541 ggagaacgcc gcgtggccaa accgatcctg atgggcgccg tcgtgctctt ccccatcggc
      601 gccgtcttcg cctatggcgt catcgccctg ctgctgcgcg tcatgcaaca ctatgccccg
      661 ccggatatcg atccactgct taatatattt gattatttac gagttatgac aacaatgatg
      721 ttggtctttg gagttgtttt cgaacttcca ctgattgtcg cgatcgccgc ccgcgtcggc
      781 ctcgtcaccc ccgcctggct ccagacctat cgccgccatg cctacgtcgt cctcgccgtc
      841 gccgccatgg tcctgacccc cgccgacccc tacagcatgc tcatcgccct cgtccccctc
      901 atcggcctct atgaactgtc cgtcgccctg gcgcggccga tggccctgct ccacgaacgg
      961 gacgccagga ccgcggcttt cgtcgactga gcccatctgc gcattcgact tgccgccaac
     1021 gcgcgtgcta cagttccccc atcgccctga tcgccgcggc tggcggcggg aggcccccca
     1081 tgcaggcact tgtgaaattc atcgcaatcg ccattttggc cacgtttggc ttgaccggct
     1141 gctcctttca tctgttcgga ccgccgaagg atacgttcta cgaaaccgtg ctcgaccgct
     1201 ttcccgacaa ccgcgcggcc ctcttcggcc agggccaggt tcttctccgc aacggccagt
     1261 accaggaagc cctgccttat ctgaagcgcc tgacccggct ggagccggac aaccagcagg
     1321 tgtggctgac gctgggccgg gcctatctcg aggcacgcga tggcctccgc gcggagcggg
     1381 cgttccgccg ggccattggc atccggccga cgatggacgg cgagttgggt ctcggcaacg
     1441 ccctcatgct ccagggcgat ctgagcgccg cccggtccgt cgccgagcgc atcgcggtaa
     1501 cctacggcga atcggcttac ttgtggcgtc tgcgcggcga tattgccttc atggccgatg
     1561 atccggctga ggcggcccgg gcctacaacg aaagcctcgg actcgacccg gcccagccca
     1621 gcgtccagga acgcctggcc gatctttcgg ccctggttca agttagtcaa cattaggagg
     1681 gtgtttcacg tggaacgacg gacgggcgat gtttcacgtg gaacgcaatg cggatgaccc
     1741 agacaatcgc gatctccaat cagaaaggcg gcgtcggcaa aacgaccacc gccgtcaact
     1801 tggccgcctg cctggccgat ctcggccacc ggaccctgct cgtcgatctc gacccccaat
     1861 ctaacgcctc cagcgccctc ggcttcgact ccaatcgcct ggacgcctcg atctacgacg
     1921 cgcttctcga tcacgccgcg ccgaccgtcc atcctctcga cgcccagcat tccaatctct
     1981 tccttctccc ctcctcgatg gatctggccg gcgccgaatt cgaactcatc gaccgcgagg
     2041 ggcgcgaatc cgccctgcgc gacgccctcg gccgcctcga ccagcccttc gactacatcc
     2101 tcatcgattc gccccccagt ctcggcctcc tcacgctcaa tgccctggtt gccgcccgct
     2161 gggtcctcat ccccgtccag gccgaatatc tcgcgctcga gggtctctcc cgcatggttc
     2221 agaccatcca gcgcatcaac cgcaagcaga atccggagct cgaaatcctc ggtctgctcg
     2281 ccacgctcta cgactcgcgg accaacctgg cgcatcaggt cctggaggag ctccgccgcg
     2341 cgttccccga caaggttttc tccacccccg tcacccgttc cgtccggctg agcgaggccc
     2401 ccagttacgg ccagaccatc ctgcgctacg acgcccgctc gtccggagcc ctgcaatacc
     2461 aacaaatcgc ccaggaggtg atccatgtct gtgaaaaagc gagccttggg tcgagggctt
     2521 gacgcgctgc tgggcgccga cagcgcacca accgccgtgg cgaccgtccc ggacgtcccc
     2581 gcctccgcga ttgtgatgat cgacgtcagc cgcatcacgt ccaaccccta ccagccgcgg
     2641 cgccacttcg ccccggaagc cctggaggaa ctcgccgcct cgatcgagca gaactgcatc
     2701 ctgcagccgc tcgtcgtccg ccgcgccgcc gccgccggcc agtttgaact cgtcgccggc
     2761 gaacgccgct ggcgcgcggc ccagcggctt gccctcgccg aggtgccggc catcgtccgc
     2821 gagtataccg accgcgaaat gctcgagctg gcgctgatcg aaaacctgca gcgtgaagag
     2881 ctgaacgccg tcgaggaagc gcgggcctat cgccgtctga tcgacgaatt cggcctgacg
     2941 caggaacagg ttgccgagcg cgtcgggaag tcccgcgtcg ccgtcaccaa ctcgctccga
     3001 ctcctgcgcc tctcggaccg cctcctgggc tggctggacg atggttcgct ctcggcgggc
     3061 catgcacgcg ccttgctagg cttggataac gagggcttgc agttggcttt ggcccgtgaa
     3121 atcatgacca aagagttgag tgtccgcgag accgaacgcc gcgtccgcac gctcaatcag
     3181 gccccgaacc cccaacccaa ggcccagact cccaccgtgg acatcgacac ggccgacctg
     3241 gaagaaaaac tgacgctcca cctgggactc caggtcaaga tccacccccg gacaaacacg
     3301 accggccgga tcgaggtctt ctattcgtcg ctggatgagt tcagtcgcgt cttcgatcac
     3361 cttggaatct ctctggaaca ggaactgtga acccatgaaa gcccgcttcg gctcgcaccc
     3421 gtccggccgc gatcttgact acctcccgcc agcgtcacgc taccgcatcg cgctgatgtt
     3481 cctcctcttt ctcgtcggca cagcgaccat cgcgctggcc cagaccgccc ccaactccaa
     3541 tctgcagaac tggatcggcg tcgatccgat cgccatgctg tatgactcgg acatcatcgg
     3601 tcaggcgtgt ctgattattc tggccggttt ttcgatcatc agttggatgg tgattgtcta
     3661 taagtatctc cacatccgcc aggcgagcac ccagaccgac gcctttgtcg aagtctgcaa
     3721 ccagggcagc ggcgaactgg aggaggcctt ccgcctggct tcaaacttcc ctgattcccc
     3781 gctggcccag atcctccgcg aaggctacct cgaactggag atcgaaaact ggtatcgcga
     3841 cggctacgac ctggatcccc aagcgcgcct ggacatcatc aaggtcagtg ttgaacgcgt
     3901 cttcgaacgg acgatcacca gtgaaatcaa tcatctggaa agcaaactgg tcttcctcgc
     3961 cacgacctcc agcgtctgcc cgttcatcgg actgtttggt acggtctggg ggatcatggg
     4021 aacatttcag acggccatga gcggcagcca agccgtggcg ctcagttcgc tggcgccggg
     4081 catctccacc gcgctgctca caaccgtcgg cggcctgttc tgcgccatcc ccgcgtccgt
     4141 catgtataac tacttgacgc accgcgttcg catgctcatc tcgcgcatgg acgccttcgc
     4201 cctcgagctg ggcaacgtcg tccagaaaca ggtcatgaag cagggggcga tggtctgaac
     4261 catcgaggga gcaaccgaca tgcgccgaca ccgccgcgat ctcgaagtcc tttccgacat
     4321 gaacctgacg aacctgctgg atacggcgtt cattctgctc atggcgttca tgctggttgc
     4381 gccgatgctc aagaccggca tcgacctcaa cctgccgcga gtcggcgccg gccagctcca
     4441 aggtcagcag aaaaccgtca ccatttcgat cgccaagcct caggtcgaag gccagtccga
     4501 gcgaatctac gtggatgacg cgcgcgtcac ctacgaagag ctcgaggaga aactggccga
     4561 gaccaagagc aattaccaag ccttcagcgt ggcgattgaa gccgacgccg aggccacgta
     4621 tcaaagcgtc gcccgcgtcc tctctctgct caaggcgatg cagatcacgt ccgtcgggct
     4681 tgtcaccgac ccggccgcct ccaaccagca ggccggctcc gccgccgccc ccgagcccga
     4741 ggatcaatcg gagtagcgac tcgtgacgcg ctcgctgttc gtctccatct tcgtgcactg
     4801 cctgctggtg gtggggctgc tcgtcctgag ccggctggcg ccccccgtcg aacggatgga
     4861 gtcgctgacg gtcgagttga tcagtgcgat gccgctcccc gccgagcccc tgccggtggc
     4921 cacgccggaa cccgacatcg agccgacccc cgagccgacg ccgcggccga cgcccgagcc
     4981 cacccccgag ccaaccccgg aacccacgcc ggagccgacg cctgagccca cgcccgaacc
     5041 gaccccggaa ccgacgccgg aaattgtcat tcccaaaccg accgagacgc cgacgccgac
     5101 ccccacgccc acccccaaac cgacgcccaa ggcgacgccc aaacctacgc cgacgccggc
     5161 gccgaagaag agccgcgacc ccaaggaaaa actcgacgac gccaaggtcc tggaggagga
     5221 gcccgagccg acgcccaaac ccacgccgaa agccacgccc aaaccgccgc cgccccccgt
     5281 cccggtcatc acgcccgaac cggaaccggc gcccgcgatt cccagcttgc cttcgaccga
     5341 cgagccggtg cgccgcgtcg acgccaaggc tggcgagtcg gtcagcatcc aggccagcgg
     5401 cgagggcaac ctggccatga cgtatctggt gcaggtgcgc cagcgcatcg aagccaactt
     5461 ctcgcatccg ttttcgaaca ccggccgccg cgccacggtg gaattcacca tccagcgcga
     5521 cggcacgatc agccgcgact tcaaaatcat ccagtcggcc ggccccagca tggaccacta
     5581 cgcgatccag gcgctcgaac ggacgaaaga tctgccgccg ctttacgaca gctacaccaa
     5641 gcctttcctg cgctgccaga ttacgtttga attcaaccaa aagaactgag cggcgcggcg
     5701 ccgcggcgcg atggtctttt cgctgcgcaa gccggcgcgc gtgtgattga atgggcggcg
     5761 cgagtccgcc ccgcccttga accgcacccg aggtaggaag atggcccttt ccgcgtttga
     5821 tttcgagcag ccggtgatcg acctggaaaa tgagctcaag gcgctccagg aactgaaccc
     5881 gccgcccgac aacctggcgg aacaggtcag cgccctgcgc gagcggatcg agcgcacgcg
     5941 ccgcgagatc tacgacaacc tcagcgcctg gcagatcctg cagatcgcgc gccaccaggc
     6001 gcggccccgc gcattggact acgtccgccg catcttcacc gactggaccg agctgcacgg
     6061 cgaccgggcg tatggggacg accatgcctg catcgccggc ttcgccctgc tcgacgagca
     6121 accggtctgc ctcatcggcc agcagaaggg gctcgacgtg cgcgacaacg cctaccgcaa
     6181 ctacgggatg atgcacccgg aaggctaccg caaggcgttg cgcgtgatgc ggctcgccga
     6241 gaaattcggc cggccggtga tctgcctgct cgacacggcc ggggcctttc ccggcgtggg
     6301 ggcggaggag cgcggccagg ccgaggcgat cgcgcgcaac atcctcgaga tgtcgaccct
     6361 gcgcacgccg gtcatctgca tcgtgattgg cgaggggggc agcggcggcg cgctcggcat
     6421 cggcgtcggc aaccgcctcc tgatgcagca gtatgcgttt tacagcctga tctcgccgga
     6481 gggctgcgcc tcgatcctgt ggcgcgacgc gaagttcgcc cccgaggcgg cccgcgcgct
     6541 caagttcacc gcccgcgacc tgaaggagct gggcgtcatc gacgagatca tcccggagcc
     6601 ggaggggggc gcgcaccgcg acgcgaacat ggcggcgcgc ctggtcggcc aggcgatccg
     6661 cggccacctg gcccagttgc gcgcgatgtc gatcgacgag atcgtcgagg accggttcca
     6721 gaaattccgc cggatcggcc agtgggagga aatcgaaacc gccccctgag cgaccttgtt
     6781 cattccccag actgcaagct gtttagcgat tccaggttca gcgccgttga ccggttcagt
     6841 ccgcggaacg gacggccgga atacagcgca ggatgacggc cacaggccgg aacctgcgtc
     6901 gcctccccct accgcccaaa cccctgcaag cggtggccgg agagatggcg ccatccgaca
     6961 ggagtaccgc caacagggcc tcactgaacg ccgaggatag gatgatcttg tcattgctgc
     7021 agggtgcaga accgtttctc ggcggagtca ccggatccaa agcggcgccg g
//
LOCUS       DAOPCO010000205         7065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9547_length_7065_cov_4.526248, whole genome shotgun sequence.
ACCESSION   DAOPCO010000205 DAOPCO010000000
VERSION     DAOPCO010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9547_length_7065_cov_4.526248"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            524..1516
                     /locus_tag="PLS90_12770"
     CDS             524..1516
                     /locus_tag="PLS90_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPK03319.1"
                     /translation="MPRAYYNSRLYAIAGDPTSPRFEFLPTYPDLVNNGQDEEFSRYY
                     NFGVLALDGRLYQFLSTPNRPLLQPEPRFVGSKLIYSTDGGASWRNQDGSSPVRWEAW
                     AERSRTNMVFFEEEGDAFSLLSVLQMGRNYSLNRDGFVYVYAPNGNTEGRMNQLVMFR
                     VRREALLNRGAYEFFAGLGPDGGARWSPRIEDRAPTYEFPAGYVNRAIHPYAWQPSVV
                     YYAPLETYLMANWGMGCGDDGMWFGRPSYLGFWTAPAPWGPWTQVHEETAWMPAGDRN
                     ARAYQPQIAPRWIAPDGRSFWLVWTDFQETKDAEGNVLRPYYAFNAQKVLVHSK"
     gene            complement(1482..2870)
                     /locus_tag="PLS90_12775"
     CDS             complement(1482..2870)
                     /locus_tag="PLS90_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03320.1"
                     /translation="MCSKLLHATNIQKSNWCRPMQSVSQNLNKSSRHFVLWLFLTICA
                     CCPCSSLCSAELTSGERQVVLNPYQSIDWATVGRYKAALHVHTLQSDGRYMPDEVIHA
                     YRDAGFKILAITDHDWHHPNLHVLNGNLSGTRWSPYPLAPIPANYPANTTWPWTSFGG
                     SDPSTLGMVGIQAGELTFRHHINSYFSDYGVWYERTGRDAPYGGIVDATGREVWEDDQ
                     LIAIRDKGGLAILNHPGLPDSFTWWERKPLDWYVERFQIHDPSYLVGMEVTNAAPKHE
                     LYDEGLWDQLLSRFMPHRPIWGFGNDDNHVLKTKISYNLFLMDELTSASVRKALLDGQ
                     FLFTKSTAHADYTSSNVNVDVFPIVKSITLDPAAGTITIDAAGHDEIRWISSPMSTSP
                     SEDYRTSDTPWDQGRVVHTGATLDLRRTPHIRHYVRAEILRRDGESLHRVFTNPFGIA
                     EVTSNGPAPSAR"
     gene            complement(2901..4028)
                     /locus_tag="PLS90_12780"
     CDS             complement(2901..4028)
                     /locus_tag="PLS90_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK03321.1"
                     /translation="MNTILDPSRAIDLALARHVVLARGALSSSTGDPDRPWRPPLLRR
                     SEGFAASALVLGDEALAQEPQSARDAIAAALASFQQARGTPPRALKIGERAAFELEVP
                     SPTAAGALAGRIALVTGAAGAIGHGLCRGLLEQGCRVAATDLAGEALDQTASAFRAEF
                     GDNILGLPMDVARPEAVREAFAALGLQWGGVDLVIVNAGVAHVAALEELELAEFQRLD
                     KVNVEGTLNTLAEAGRWLKHQAIGGDIVLISTKNVFAPGARFGAYSATKAAAHQLARI
                     AALEFAEHDIRVNMVAPDAVFSAGDVRSGLWASVGPDRMKARGLDEAGLEEYYRGRNL
                     LKARVTARHVANAVLFFATRQTPTAGATLPVDGGLPDAAPR"
     gene            complement(4122..5030)
                     /gene="lsrF"
                     /locus_tag="PLS90_12785"
     CDS             complement(4122..5030)
                     /gene="lsrF"
                     /locus_tag="PLS90_12785"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HPK03322.1"
                     /translation="MPEADATQPKRFHPDIPQRSDGFFLKGASSLDWGMKHRMARIFR
                     PDSGRTLMLAIDHGYFQGPTTGLERIDLNIVPLLGATDALMLTRGILRAVIPPSYPRG
                     IVMRCSGGPSILKELSNEELAVDVEDAIRMDVAAMTLQVFVGGEYETRTIHNLTRLVD
                     LGLRYSIPVMAVTAVGRELVRDAKYLRLACRICAELGAQFVKTYYCDEEFETVTASCP
                     VPIVMAGGKKLPELEALTMAWNALERGAAGVDMGRNIFQSDAPIAMLHAVRKIVHEGY
                     RPKEALEVYEELKHAATRADRQPVGA"
     gene            complement(5098..6861)
                     /locus_tag="PLS90_12790"
     CDS             complement(5098..6861)
                     /locus_tag="PLS90_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HPK03323.1"
                     /translation="MDRLTRRGFLGKATTLGAAAILSPTLLHCARGGMPLPQGQRPNI
                     LLVITDDQGFGDFGCYGNEVLNTPHLDRLHAESTRLERFYACPLCTPTRASLMTGRYN
                     YRTGAWDTWKGRASLSSDEVTIAEILAANGYRTGIFGKWHLGYSAPTRPQDQGFQETY
                     TWDGVSKGRVDPPIEHNGVLEERQGFITDLIFDQCMSWMEENEDGPFFAYLATFLPHD
                     IVGVSVPEEYVAPFRDVEGLTPGDQVIYGMIAKIDENMGRLLQFLEQSGRFRDTIVIF
                     MSDNGPTRLTEDLTSEPERIASQKTEIGARFNRGLRGGKATLYEGGIRIPFWIRLPGE
                     SAAGREIDAPTAHIDVLPTLLDLCSVKPAADNPPIDGRSFAPLLQGTGDWPDRCLFIQ
                     NHRVEAPEPGVEYCVVGRQYKLIENDFGLQLYDLHADPGETTNIAGEHPEVVEQMRDA
                     YRKWYADVTSGRGFYPALTALGDPTQPLIDFPWYTRHSVGWPVRIQRQGPYTVSVKGI
                     HHAIFKGETAFELKLGDITLNRVVDPGADTLVFEGLTPTLGESTFDLKAAGPIEEKEI
                     FYGANDPGFDHVTIEYVGPPR"
BASE COUNT         1318 a         2074 c         2359 g         1314 t
ORIGIN      
        1 agctgagcga cgaggcggat gagaaacagc cgaggggcga tggcggggat tcgaatgggc
       61 aagacgcgcg cataaccagg aggcgaaatg tggatcagcg catccgaaaa tgtctgaccc
      121 gagccagaga aaaaaagtat acattttaag ccgcggcggc gcaagcgacg atgggataag
      181 agggctatgg cccttgggag cgaaaagaga aagacgacgt ggaggggacg ggtttcccga
      241 tcgcccgccg cggcggagcc gcaggatgcc gcgaaagccg gagcttgatc ctgaacgcac
      301 gcgatctgta tattcaatcg gaccagccgg ccgggcacat tgagcaggcg gctcgtgaag
      361 tgagaatcca aggccgaggg atggcatgag gcgaattgat catcttgaac gacttgacga
      421 tacgacgctc cgactgggcg gaaacggcga caactggcat atgacgtggg ccgatgatgg
      481 ccgtcaatat gtatgtcttt gtgacgggac gggctgggac ggaatgccgc gcgcctatta
      541 caacagtcgg ctctatgcga ttgcggggga tccgacgtcg ccgcgcttcg agtttctgcc
      601 cacttacccc gacctggtga ataacgggca ggatgaggaa ttcagccgtt actacaactt
      661 cggcgtgctg gcgctggatg ggcgcctcta ccagttcctg agcacgccga atcggcccct
      721 gttgcaaccg gagccccgct ttgtcgggtc aaagctgatc tactcgaccg acggcggcgc
      781 gtcctggcgc aatcaggacg gatcctcccc ggtcagatgg gaggcctggg ccgagcgttc
      841 gagaaccaac atggtcttct ttgaggaaga gggtgatgcg ttctccctgc tgagcgtgct
      901 gcagatgggg cgcaattatt cgctcaatcg ggacggattt gtttatgtct acgcccccaa
      961 tggaaatacg gaaggccgga tgaaccaact ggtcatgttt cgcgtccggc gcgaggcgct
     1021 gctgaatcgc ggggcttacg agtttttcgc cgggctgggt cccgacggcg gggcgcgctg
     1081 gtcgccccgg atcgaagacc gggcgccgac gtacgagttt ccggccggtt acgtcaaccg
     1141 ggcgatccat ccctacgcct ggcagccaag cgtggtgtat tacgcgccgc tggagaccta
     1201 tctgatggcg aactggggga tggggtgcgg cgacgacggc atgtggttcg gccggccgag
     1261 ttacctggga ttctggacgg cgccggcgcc ctggggccca tggacgcagg tccatgagga
     1321 aacggcgtgg atgcccgcgg gggatcggaa cgcgcgcgcc tatcagccgc agatcgcgcc
     1381 gcgctggatt gctcccgacg gccgctcgtt ctggctggtc tggacggact ttcaggagac
     1441 gaaggatgcg gaaggaaacg tcctccggcc gtactatgcc ttcaacgcgc agaaggtgct
     1501 ggtccattcg aagtgacctc ggcgatcccg aacgggttgg tgaagacgcg gtgcaaggat
     1561 tcgccgtcgc ggcgaaggat ttccgcccgg acgtaatggc gaatgtgggg cgtcctccgc
     1621 aaatccaacg tcgcgcccgt atgcacaacc cttccttgat cccaaggcgt gtcactggtc
     1681 cggtagtcct cagatggact cgttgacatg ggggatgaaa tccatcgaat ttcgtcgtgg
     1741 cccgcggcat caatcgtgat cgtgccggcg gcgggatcca gcgtaatcga tttgacgatt
     1801 ggaaagacgt ccacattgac gttgctggaa gtatagtcgg catgagccgt cgacttggtg
     1861 aacagaaact gtccatccag caacgccttg cgcacgctcg cggatgtcag ttcgtccatc
     1921 aggaagagat tataggaaat cttcgttttg agtacgtggt tgtcgtcgtt gccgaagccc
     1981 cagatcgggc gatgcggcat gaagcgactc agcagttgat cccaaaggcc ctcgtcgtag
     2041 agttcatgct tcggagccgc attggtgact tccattccca ccagataaga gggatcgtga
     2101 atttgaaaac gttcaacata ccaatcgagc ggctttcttt cccaccatgt gaaggaatcg
     2161 gggaggccgg gatgattcaa tatcgccagt ccgcccttgt cgcgaattgc aatcagctga
     2221 tcatcctccc acacctcgcg tccggtcgca tccacaatgc cgccgtatgg cgcatcccgg
     2281 cccgtgcgtt cgtaccagac tccgtagtcg ctgaagtagc tgttgatatg gtgtctgaac
     2341 gtcagttcac ccgcctggat gccaaccatc cccagagtgg atggatcact gccgccgaag
     2401 ctcgtccagg gccacgttgt gttggccggg taattggcgg gaatcggcgc cagcgggtag
     2461 ggactccatc tggttcctga cagattacca ttcaagacgt gaaggttggg atggtgccag
     2521 tcgtgatccg tgatggcgag gatcttgaag ccggcatcgc gataggcatg gatgacttcg
     2581 tccggcatgt agcgcccgtc gctttgcagc gtatggacgt gcagggccgc cttgtagcgt
     2641 ccgacggtcg cccagtcgat cgattggtac gggtttaaga cgacctgacg ttctccagat
     2701 gtcagttccg ccgagcacag cgaggagcac gggcagcagg cgcaaatcgt cagaaacagc
     2761 cataacacga aatgcctcga actcttattc aggttctggg acacagattg catgggcctg
     2821 caccagtttg atttctggat attcgtggca tgcagcagtt tgctgcacat cagggatttt
     2881 cgctgactcc gagcccgggg ctatcgcggc gccgcatcgg gcaggccgcc gtcaacgggc
     2941 agggtggcgc cggccgtggg cgtctggcgg gttgcgaaga acaggacggc gttggcgaca
     3001 tggcgtgccg tgacgcgcgc cttcagcagg ttgcgcccgc gatagtactc ctcgagcccg
     3061 gcctcatcca gtccccgggc tttcatccgg tccggtccaa ccgatgccca cagaccggag
     3121 cgcacgtcgc cggcgctgaa caccgcatcg ggagcaacca tattgacgcg gatgtcgtgc
     3181 tcggcgaact cgagcgcggc aatccgggca agctggtgcg cggcggcctt cgtcgcgcta
     3241 taggccccga agcgagcgcc gggcgcaaag acgtttttcg tcgagatcag gacgatatcg
     3301 ccgccaatgg cttggtgctt gagccagcga ccggcttccg ccaacgtgtt gagcgttccc
     3361 tcgacgttga ccttgtcgag gcgctggaat tcggcaagct cgagctcctc cagcgcggcg
     3421 acgtgcgcga cgccggcgtt gacgatcacc agatcgacgc cgccccattg gagcccgagc
     3481 gcggcgaagg cttcgcgcac ggcttcgggc cgggcgacat ccatgggaag cccaaggatg
     3541 ttgtcgccga actccgcacg gaaggccgaa gccgtctggt caagtgcctc gccggcaaga
     3601 tcggtcgccg cgacgcggca cccctgctcc aggagccccc ggcacagacc gtgaccaatt
     3661 gccccggcgg cccccgtgac caacgcgatt cgcccggcga gcgcaccggc ggcggtcggc
     3721 gacggcactt ccagttcgaa agcggcgcgc tcgccgattt tcagcgcgcg cggaggagtt
     3781 ccccgcgcct gctgaaatga agcgagcgcc gccgcaatgg catcccgcgc agattggggc
     3841 tcctgggcta gcgcctcgtc gccgagcacg agggccgagg ctgcgaaccc ctcgctccgg
     3901 cgcaggagcg gcggccgcca cggccgatcg ggatccccgg ttgaggacga gagcgcgccc
     3961 cgcgccaaca ccacatgccg ggcaagcgcc aggtcgatag cgcgggatgg atcaagaatg
     4021 gtgttcacga gcggctcacc ttccggatct gtggccttcg ggttctggag gaggttcgtc
     4081 ccggcgcgaa attcccattc gcggcgatga tggaggacga attaagcgcc gaccggctgc
     4141 cggtcggcgc gcgtagcagc gtgtttcagc tcttcgtaga cttccagggc ttccttgggc
     4201 cgatagccct catgcacgat cttgcggacc gcgtgcagca tcgcgatggg cgcgtcgctc
     4261 tggaagatat tgcggcccat gtcgacaccg gcggcgccgc gttcgagcgc gttccaggcc
     4321 atggtcaacg cttcaagttc ggggagcttc ttgccgcccg ccatcacgat cggcaccggg
     4381 cagctcgcag tcacggtctc gaactcctcg tcgcagtagt aggttttgac gaactgggcg
     4441 cccagctcgg cgcagatccg acaggcgaga cgcaggtact tggcgtcgcg gaccagttcg
     4501 cggccgacgg cggtgacggc catcaccgga atcgagtagc gcaggcccag gtcgaccagc
     4561 cgcgtcagat tgtggatcgt gcgggtttcg tattctccgc cgacgaagac ctgaagcgtc
     4621 atcgcggcga catccatgcg gatggcatct tcgacatcga cggcgagttc ctcgttggag
     4681 agttccttga gaatcgaagg gccgccgctg cagcgcatca cgatcccgcg cgggtaggat
     4741 ggcggaatga ccgcgcgcag aatcccgcgg gtcagcatca gggcgtcggt cgcgccgagc
     4801 aagggcacga tattcaggtc gatgcgctcg agtccggtgg tcggaccctg gaaatagccg
     4861 tgatcaatgg cgagcatcag ggtgcggccg gaatccgggc ggaagatccg ggccatgcgg
     4921 tgtttcatgc cccaatcgag tgacgatgca cctttaagaa aaaatccgtc cgaccgttgg
     4981 ggaatgtcgg gatgaaagcg cttgggctga gtggcgtctg cttccggcat ctgagctacc
     5041 tccgaaaatc aaatggggtg gatttacatg ggttggtccg cggcggcatg cgaccgttca
     5101 gcgcggcgga cccacatact cgatcgtcac atggtcgaag cccgggtcgt tcgcgccata
     5161 gaagatctcc ttttcttcga tcgggccggc ggccttcaga tcgaaggttg actcgccgag
     5221 ggtgggtgtg agcccttcga aaaccagcgt atcggcgccc ggatcgacta cccggttcag
     5281 cgtgatatcg cccagtttca gttcaaacgc cgtctcaccc ttgaaaattg catggtggat
     5341 gcccttgacg ctgacggtgt aggggccctg gcgctgaatc ctgacgggcc acccgaccga
     5401 atgtcgcgta taccacggga agtcgatcag cggctgcgtc gggtcgccca gggccgtgag
     5461 ggcgggatag aatccccgcc ccgaggttac atccgcatac cacttgcggt aggcgtcgcg
     5521 catctgctcc acgacctcgg gatgttcgcc ggcgatgttc gttgtctcgc cgggatcggc
     5581 atgcaggtcg tagagttgaa ggccgaagtc gttttcgatc agtttgtatt gccggccgac
     5641 gacgcaatac tcgacgccgg gttcgggcgc ttcgacgcgg tgattctgga tgaagagaca
     5701 gcggtcgggc cagtcgccgg tcccctggag gaggggagca aacgagcgcc cgtcgatggg
     5761 gggattgtcc gccgcgggct tcaccgagca aaggtcgagc agggtgggca ggacgtcgat
     5821 atgcgccgtc ggggcatcga tctcgcggcc cgccgcggat tcgcccggca gcctaatcca
     5881 gaacgggatg cgaattcccc cctcatagag ggtggccttt ccgccgcgga ggccgcggtt
     5941 gaagcgcgcc ccgatctccg tcttttgcga cgcgatccgc tccggctcac tggtgagatc
     6001 ctcggtgagg cgcgtcggac cattgtcgga catgaagatc acaatcgtgt cccgaaagcg
     6061 gccggactgt tcaaggaact gaagcagtcg tcccatgttt tcgtcgatct tggcgatcat
     6121 cccatagatc acctgatcgc ccggcgtcag gccttcgacg tcgcggaagg gcgccacgta
     6181 ctcctcgggg accgagacac ccacgatgtc gtgcggcagg aaggtggcca ggtaggcgaa
     6241 gaaggggccg tcctcgttct cctccatcca gctcatgcat tgatcaaaga tgaggtcggt
     6301 gatgaagccc tggcgctctt cgagcacgcc attgtgctcg atcggggggt ccacgcggcc
     6361 cttgctgacg ccgtcccagg tataggtttc ctgaaagccc tgatcctgcg ggcgcgtggg
     6421 ggcgctatag ccgaggtgcc atttgccgaa gatgccggtg cggtagccat tggccgcgag
     6481 aatttccgcg attgtcacct catcggagct caggctggcc cggcctttcc aggtatccca
     6541 ggcgccggtg cgataattgt agcggccggt catcagcgag gcgcgagtcg gggtgcagag
     6601 cgggcaggcg tagaatcgct ccagacgagt gctttcggcg tgcagccggt ccagatgggg
     6661 ggtgttgaga acctcgttgc catagcagcc gaaatcgccg aagccctgat cgtcggtgat
     6721 gacgaggagg atattggggc gttggccctg gggcagcggc atgccgccgc gggcgcaatg
     6781 gagcagggtc ggcgagagga tggcggcggc tccgagcgtt gtggccttgc cgagaaagcc
     6841 gcggcgggtc agtcggtcca tgacggcgtc tccagtggca gaagatcccg tttcccgcga
     6901 gtcaggaaac ggggcgatag catggggaat ccagctccct catgaattgg gcggcatcga
     6961 ggcgaggatc attggtcgga ggcgcgccat cgatgaatgc ttccatcgcg ctccagtcgg
     7021 gcaagccttg ctgcagggcg gcaagcagcg cggtgtctat cggca
//
LOCUS       DAOPCO010000206         7053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9568_length_7053_cov_5.158760, whole genome shotgun sequence.
ACCESSION   DAOPCO010000206 DAOPCO010000000
VERSION     DAOPCO010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9568_length_7053_cov_5.158760"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /gene="terL"
                     /locus_tag="PLS90_12795"
     CDS             complement(<1..1688)
                     /gene="terL"
                     /locus_tag="PLS90_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HPK03324.1"
                     /translation="MPHPHPPPDTPQTPPALPPPPPLDPPAAAPPPASADAFRHDPRG
                     FAETFFADLCGLPWNRLHRHLFERRAARGLVRPERRQGSIEVTLAPRGAAKSTLVSLI
                     FPIHALLHGLEPYIVLIAATLGQARRLLTHLRRALISGEPLRRRYAEVFAAGFRHTES
                     TVELGGARVDAFSAGSEIRGIRHGAWRPTWIILDDIERSDRVHSPRYRDWLADWFREV
                     IEPLGAAGTNIDLVGTLLHRDALPARLMRRPDVAAAVFRAILREADDQQLWDAWRTRL
                     SDLSNPARLATARAFYEAHRPQMDRGAEVLWEPAEDYYDLCLAREVRGRAAFDQEKQN
                     DPWGDDHATFDLRLLRRFRLAGGDLICDPPRWREPGPGEPADGLAPRVALSALRRFGF
                     LDPALGGRRGDFAAIATLGADAHGYLYLLDLWLERATPSAQVDRLFTLHETWSYDLFG
                     VEANASQKILLDLIERERASRRQRRRPWRVPVSDFVQRGAKQARIAALEPFIRAGWLL
                     FNADLPDEFLRQLREFPQGEHDDGPDALAAAVDLARHATLPHDSLLTRPRPRRP"
     gene            complement(1842..2168)
                     /locus_tag="PLS90_12800"
     CDS             complement(1842..2168)
                     /locus_tag="PLS90_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03325.1"
                     /translation="MSNPQGPLLRILDFGPKTLRVGQRVNPQPDGSSAMWIRLTQPVG
                     RLPLTVVFAPRHRIPVCVDGSDTVTAVIPPSLQSTPGYYPFYVTIESTRRKSDERVLI
                     VEPAPD"
     gene            2489..2980
                     /gene="ispF"
                     /locus_tag="PLS90_12805"
     CDS             2489..2980
                     /gene="ispF"
                     /locus_tag="PLS90_12805"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPK03326.1"
                     /translation="MQAESPLRVGQGFDVHAFAAGRALILGGVRLDHARGLLGHSDAD
                     VLAHAITDALLGAAGRGDIGHHFPDTDAQWKDADSLELLRRVAAELAGAGWGIVNVDA
                     TVIAQAPKLAPHLPAMVKRLAEALGVAPERVNLKATTSERLGFTGREEGIAAQAVALL
                     GRR"
     gene            3249..4601
                     /locus_tag="PLS90_12810"
     CDS             3249..4601
                     /locus_tag="PLS90_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HPK03327.1"
                     /translation="MPRRRNPNAPDPVDERLAEKLAASIAQARLAEKIQARGRPPVPG
                     QHVAGPDLWTVLYFSLWLTAALLAALVAFGRPIDPLLGLAPRDLQPLTSGRMIELVGW
                     TLGLGLTAWLAARNRIEMHDLLVGLSVKERLVPLVFTLVFFLTPMFPESTQVLRHGFL
                     WATPFLLLLAATPSMTRRLLAWMLAGAWFCAGGVGGAAGAALVLGFAAAWLLALGATH
                     FAYLGDPHGLGGWWPARRLARNTVLTALPALLAAVVVWWAWPQRMRRGGGATDAAGPG
                     APPGARPTPAMDGFDAAEFVYRGVLGILAIIALVILMVLLRRFFRRRLRVRMEGDFLP
                     GQIGQLEQRPLEDAPARPRLSGLRGRIVGQWARWARAMGREVEGRRAGESAAEFAARL
                     ARENSAAAPPAELTALLERAHYGETEPAPADAETMRRLVQEELSRQSLRRQQPVDEGE
                     "
     gene            complement(4625..5047)
                     /gene="ndk"
                     /locus_tag="PLS90_12815"
     CDS             complement(4625..5047)
                     /gene="ndk"
                     /locus_tag="PLS90_12815"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPK03328.1"
                     /translation="MALERTLVLVKPDGVRRGLVGEIIGRFERKGLRIAGLRMLRFDD
                     ALAQRHYAEHVEKPFFPELKEFITSGPVVAIAVEGDMAIEVVRAMMGKTKFFEAAPGT
                     IRGDLAFSTTENLVHGSDSPASAARELPLFFTEDQLFV"
     gene            complement(5309..6298)
                     /gene="plsX"
                     /locus_tag="PLS90_12820"
     CDS             complement(5309..6298)
                     /gene="plsX"
                     /locus_tag="PLS90_12820"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPK03329.1"
                     /translation="MRIALDAMAGDFGPEPLVKGAIQAVEETRHHVILVGDAETLENI
                     LIREQALHPRLSIVHADTVIGMDEVPKASLRKKSSSLAVAADLVKNGEADAVVSAGNT
                     GATLAMMVTKWRPLPGVSRPSLATIIPVPHHPVVLLDVGANIDCRPQHLYDFGVMGSI
                     YAREIIGRRSPRVGLLNIGSEDGKGTDILQQTFKLLADSPLNFIGNVEGGDAFTGKVD
                     VIVCDGLVGNALLKFGEGLVSMMTGHLRAELKKNLFTTLGAIPLLSTLRRFRSQLDAS
                     EYGGAPLLGVNGISIVSHGASDAHHIRSAIKTAGELVRHDVNAKIQHALNGGG"
     gene            complement(6322..6504)
                     /gene="rpmF"
                     /locus_tag="PLS90_12825"
     CDS             complement(6322..6504)
                     /gene="rpmF"
                     /locus_tag="PLS90_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPK03330.1"
                     /translation="MPVPRRRHSKSKRDKARTHKLLQAHSPSRCKNCGAARRPHQACP
                     SCGQYNGRSYRTTVTS"
BASE COUNT         1028 a         1982 c         2976 g         1067 t
ORIGIN      
        1 aaggggcggc gggggcgggg gcgggtgagc agggagtcgt gcgggagggt tgcatggcgc
       61 gccaggtcga cggcggcggc gagcgcgtcg gggccgtcgt cgtgctcgcc ctgggggaat
      121 tcgcggagct ggcgcaggaa ttcgtcgggc aggtccgcat tgaacagcag ccaaccggcg
      181 cgaataaagg gttcgagggc ggcgatgcgc gcctgtttgg cgccgcgctg gacgaagtcg
      241 ctgacgggga cgcgccacgg ccggcggcgc tgccggcgcg acgcccgctc gcgttcgatc
      301 aggtcgagca ggatcttctg cgacgcgttg gcctcgacgc caaagaggtc gtagctccac
      361 gtctcgtgca gggtgaagag gcggtcgacc tgggcgctgg gggtcgcgcg ctcgagccac
      421 aggtcgagca ggtaaaggta gccgtgggca tcggcgccga gggtggcgat ggcggcgaag
      481 tcgccgcggc gtccgccgag ggcggggtca agaaagccga agcggcgcag ggcggacaga
      541 gcgacgcgcg gggcgaggcc gtcggcgggt tcgccggggc ccggttcgcg ccagcggggg
      601 ggatcgcaga tcaggtcgcc gccggcgagg cggaagcggc gcagcagacg caggtcgaag
      661 gtggcgtggt cgtcgcccca ggggtcgttc tgcttttcct gatcgaaggc ggcgcggccg
      721 cggacttcgc gggccaggca gaggtcataa taatcctcgg ccggttccca gaggacctcg
      781 gcgccgcggt ccatctgggg gcggtgggcc tcgtaaaacg cgcgggccgt agccaggcgc
      841 gcggggttgg agaggtcgct gagccgggtg cgccatgcat cccacagttg ctggtcgtcg
      901 gcctcgcgga ggatggcgcg gaagacggcg gcggcgacgt cggggcggcg catcaggcgg
      961 gcggggaggg cgtcgcgatg gagcagtgtg ccgaccaggt cgatgttcgt gccggcggcg
     1021 ccgagcggtt cgattacctc gcggaaccag tcggcgagcc agtcgcggta gcgcggcgag
     1081 tggacgcggt cggagcgctc gatgtcgtcc agaatgatcc aggtcgggcg ccaggcgccg
     1141 tggcggatgc cgcggatttc ggagccggcg gagaaggcgt cgacgcgcgc gccgccgagc
     1201 tcgacggtcg attcggtgtg gcggaagccg gcggcgaaga cctcggcgta gcggcggcgg
     1261 aggggctcgc cggagatgag cgcgcggcgc aggtgggtca gcagacggcg cgcctggccg
     1321 agcgtcgcgg cgatgaggac gatgtagggc tccaggccgt ggagcagggc atgaatcggg
     1381 aaaatgagcg aaacgagcgt ggatttggcg gcgccgcgcg gggcgagggt gacctcgatc
     1441 gaaccctggc ggcgctcggg gcggacgagt ccgcgcgcgg cgcggcgttc gaacagatgg
     1501 cgatgcagcc ggttccaggg gagaccgcag aggtcggcga agaacgtttc ggcgaagccg
     1561 cggggatcgt ggcggaaggc atcggcggac gcggggggcg gggcggcggc gggcgggtcg
     1621 agcggcggcg gcgggggtag cgcggggggc gtttgagggg tgtcgggggg ggggtggggg
     1681 tgtggcacgg cgcgcggccc tccgccgatg ggatcggcga acggcggggc gcggccaccc
     1741 gttagaacgc acggaatatt ttagcgcaag gcgcggcggg gcggaattcc cttacgggaa
     1801 cctggcgacc cctggcggaa cagagagacg ggggggcgcg atcaatccgg cgcgggctcg
     1861 acgatcagga cgcgttcgtc ggacttgcgg cgcgtcgact cgatggtcac gtagaacggg
     1921 tagtagcccg gcgtggactg gagcgatggg gggatgacgg cggtgacggt gtcgctgccg
     1981 tcgacgcaga cgggaatgcg gtggcgcggc gcgaacacga ccgtaagcgg gaggcggccg
     2041 accggctgag tcaggcgaat ccacatggcg ctggagccgt cgggttgggg attgacgcgc
     2101 tggccgacgc ggagcgtttt ggggccgaag tcgaggatgc gcagcagcgg tccctggggg
     2161 ttggacatgt tcggctcggg ggggatggat ggaggagcgg tcatgcagac gcgcgggcga
     2221 ggcccgcagc caaaccaatg tttgaatcta aaatactgat tagcacgtct gttggtgatg
     2281 cgccagatcg aactggccga gcgggatcgg tgaagaacga aattccgttg gattcgtcgg
     2341 attggacgga tccgacggat ttcggttggg agcgaatcga ggagtcgggg cgttggcggg
     2401 cggcggcgga ttcggcttgg cggccggcgg cgcttgggtc acaatccctt ttgaccgccg
     2461 gagggcgtga gttgaccggg agggaagcat gcaagccgaa tcgccgctgc gcgtggggca
     2521 ggggttcgac gttcacgcat tcgccgcggg gcgggcgctg atcctggggg gggtgcgcct
     2581 cgatcacgcg cgcggcctgc tggggcattc ggacgccgac gtgctggcgc acgcgattac
     2641 cgacgcgctg ctgggggcgg ccgggcgggg ggacatcggg caccattttc ccgacacgga
     2701 cgcgcagtgg aaggacgccg acagcctgga gctgctgcgc cgggtggcgg cggagctggc
     2761 gggggcgggg tgggggatcg tgaatgtcga cgcgacggtg atcgcgcagg cgccgaagct
     2821 ggcgccgcat ctgccggcga tggtgaagcg gctggccgag gcgctggggg tggcgcccga
     2881 gcgggtcaat ctgaaggcga cgacgagcga acggctgggg ttcacgggtc gcgaggaagg
     2941 gattgcggcg caagccgtgg cattgctggg acgaagatga aggaccggcc gcgcggcggg
     3001 gcttgaccgg cgggggacac ggcctcatgg aatgagaagt ttgaacgcgg ccgcgtgcgg
     3061 aatccggcag atccgatgga tcggacggat tgcaagccta tcggagggcg gaccggcgag
     3121 ggcgccgggc tcacgtcaac atcaaaatca tttcgccgcg atccctagcc ggtcccacgc
     3181 gtgcaccggg cccacgccgg cggctttcgc tgtgaagcgc gcggggggct gtgcgaggat
     3241 gaactcccat gccccgccgc cgcaacccca atgccccgga cccggtcgac gagcggctgg
     3301 ccgagaagct ggcggcgtcg atcgcgcagg cgcggctggc cgagaagatc caggcgcggg
     3361 gccgcccgcc cgtgccgggg cagcatgtcg ccgggccgga cctgtggacg gtgctgtatt
     3421 tcagcctgtg gctgacggcc gcgctgctgg cggcgctggt cgccttcggg cggccgatcg
     3481 atccgctgct ggggctcgcg ccgcgggacc tgcaaccgtt gacgagcggg cggatgatcg
     3541 agctggtcgg ctggacgctg ggactcgggc tgacggcctg gctggccgcg cgcaaccgga
     3601 tagagatgca cgatctgctc gtcgggctga gcgtgaagga acgccttgtt ccgctggtgt
     3661 tcacgctcgt gttcttcctg acgccgatgt ttccggagtc gacgcaggtg ctgcgccacg
     3721 ggttcctgtg ggcgacgccg tttttgctgc tgctggcggc gacgccgtcg atgacgcggc
     3781 ggctgctggc gtggatgctg gccggggcgt ggttctgcgc ggggggcgtg gggggcgcgg
     3841 cgggcgcggc gctggtgctg gggtttgccg cggcatggct gctggcgctg ggggcgacgc
     3901 actttgccta cctgggcgat ccgcacggcc tggggggctg gtggccggcg cggcggctgg
     3961 cgcgcaacac ggtgctgacg gcgctgcccg cgctgctggc ggccgtggtg gtgtggtggg
     4021 catggccgca aaggatgcgc cgcggaggag gagcgaccga cgcggccggg ccgggggcgc
     4081 cgccgggggc gcggccgacg ccggcgatgg acggatttga cgccgccgag ttcgtctacc
     4141 gcggcgtgct cggcatcctg gcgatcatcg cgctggtcat cctgatggtg ctgctgcggc
     4201 ggttcttccg gcggcggctg cgggtgcgga tggaggggga cttcctgccg gggcagatcg
     4261 gtcagcttga gcagcggccg ctggaggacg cgccggcgcg gccgcggctg agcggactgc
     4321 gcgggcggat cgtcggccag tgggcgcgct gggcgcgggc gatgggccgc gaggtcgagg
     4381 ggcggcgggc gggggaaagc gccgcggagt tcgccgcgcg gctggcgcgc gaaaactccg
     4441 ccgccgcgcc gccggccgag ctgaccgcgc tgctggagcg ggcgcattac ggggagacgg
     4501 aaccggcgcc ggccgacgcc gaaacgatgc gccggctcgt gcaggaagag ctttcgcgcc
     4561 agagcctgcg gcgccagcag ccggtggacg agggggaatg attttgaggc cggcgcggcg
     4621 cggctcaaac gaagagctgg tcctcggtga agaagagcgg cagttcgcgc gcggccgaag
     4681 cgggggaatc ggagccgtgg acgagatttt cggtggtgga gaaggcgagg tcgccgcgga
     4741 tcgtgccggg agccgcttcg aaaaatttgg tcttgcccat catcgcgcgg acgacctcga
     4801 tcgccatgtc gccctcgacg gcgatggcga cgacggggcc gctcgtgatg aactccttga
     4861 gttcgggaaa gaagggtttt tcgacgtgtt cggcgtagtg gcgctgggcg agggcgtcat
     4921 cgaagcgcag catgcgcagg ccggcgatgc gcaggccctt gcgctcgaag cggccgatga
     4981 tttcgccgac cagtccgcgg cggacgccgt cgggtttgac cagaaccagg gttcgttcaa
     5041 gtgccatcgg gtgttcctat ctatcgtgag agttggattg atggggaagg ggcgtaaggg
     5101 acatcaggga catcagggga atcaggcgag ggattcgcga ggatttcgag tcgttctgtc
     5161 gtccctccgg gactcttgcg ctgccttgtt tccgttttcc cgccactgaa gtggctgaag
     5221 tctgccgtcc ctggggagac tggctgattg ctgagtccgg cagcgacgcc tgaggtccga
     5281 tcggtgactc aacccgatca gggggcgatt acccgccgcc gttgagggcg tgctggatct
     5341 tggcgttgac atcgtggcgg accagttcgc cggcggtttt gatcgcgctg cggatatgat
     5401 gggcgtcgga ggcgccgtgg ctgacgatgc tgatgccgtt gacgccgagc agcggggcgc
     5461 cgccgtattc cgaggcatcg agctggctgc ggaagcggcg cagcgtcgaa aggagcggga
     5521 tcgcgccgag cgtggtgaag agatttttct tcagctcggc gcgcaggtgg ccggtcatca
     5581 tcgagacgag tccctcgccg aacttgagga gcgcattgcc gacgaggccg tcgcagacga
     5641 tgacgtcgac cttgccggtg aaggcatcgc ccccctcgac gttgccgatg aaattgagcg
     5701 ggctgtcggc caggagcttg aacgtttgct ggaggatgtc ggtccccttg ccgtcctcgg
     5761 agccgatgtt gagcaggccg acgcgggggg accggcggcc gatgatctcg cgggcataga
     5821 tcgaacccat gacgccgaag tcgtagaggt gctgcgggcg gcagtcgatg ttggccccga
     5881 cgtcgagcag gacgaccggg tggtgcggga cggggatgat cgtggcgagc gaggggcgcg
     5941 agacgccggg gagcggccgc cacttggtga ccatcatggc cagggtggcg ccggtgttgc
     6001 cggccgagac gacggcatcg gcctcgccgt tcttgaccag gtcggcggcg acggcgagcg
     6061 aggaggattt cttgcgcagg gaagccttgg ggacctcatc catgccgatg acggtatcgg
     6121 cgtggacgat cgagaggcgc gggtgaagcg cctgctcgcg gatgaggatg ttttccaggg
     6181 tttcggcgtc gccgaccagg atgacgtgat ggcgggtctc ttcgaccgcc tggatcgcgc
     6241 ctttgaccag gggctccggg ccgaaatcgc ccgccatggc gtccagggcg atccgcatcg
     6301 gcggtcattc cccccgcggg ttcagctcgt cacggtcgtg cgatagctgc gcccgttgta
     6361 ttgtccgcac gaggggcagg cctggtgcgg ccgacgcgcg gcgccgcaat ttttgcagcg
     6421 gctggggctg tgggcttgga gcagcttgtg cgtgcgcgcc ttgtcgcgct tgctcttgct
     6481 gtgtcgtcgg cgcgggaccg gcatggttcg gactccgaaa gcgggggctc gccgcggcgg
     6541 tcggggcgag gcaacccgat gagatggcag cgttgggtgc gtgcgcgcgc tgcgtggggg
     6601 acggcaaaaa aagcggccgg ggcgccagcg gcttccctgg ccggatcgcc gccctcggca
     6661 gcgataacct caccatgcta cgcacggggg gcgcgatgtc aagtttttcg agggggcggg
     6721 aggcggaggc ggaaggcggg aggaggatgg ggcggggaaa tggggatccc gaaaggcacg
     6781 aaagggcctc ggggagtggg ctggaattgc ggcggcgcca gggccggcaa gcattggcgg
     6841 cggtcgcagg cggtggttga tctggcggac ggcgcgtttt cgagagccga ggacgaggga
     6901 cgagagccga gagccgaatt ccgggggggc ctttgggggc gatcacgagg acgtcgggga
     6961 gtgggctgga attgcggcgg cgccagggcc ggcaagcatg gcggcggtcg caggcggtgg
     7021 ttgatctggc ggacggcgcg ttttcgagag ccg
//
LOCUS       DAOPCO010000207         7024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9618_length_7024_cov_4.799684, whole genome shotgun sequence.
ACCESSION   DAOPCO010000207 DAOPCO010000000
VERSION     DAOPCO010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9618_length_7024_cov_4.799684"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..331)
                     /locus_tag="PLS90_12830"
     CDS             complement(53..331)
                     /locus_tag="PLS90_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HPK03331.1"
                     /translation="MDLIFEWDRSKAKENLKKHKVGFEEGKTIFNDPFLLTFPDISSS
                     EEEERFISIGLSCRERVLILIHTERQGRIRIISCRRAAARERRSYEEG"
     gene            complement(364..594)
                     /locus_tag="PLS90_12835"
     CDS             complement(364..594)
                     /locus_tag="PLS90_12835"
                     /inference="COORDINATES: protein motif:HMM:NF027602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HPK03332.1"
                     /translation="MVVLSHDIPFSGLKAGDLGAVMQVYETAALEVEFVTASGRTQVL
                     LTLRTEDVRPVRDAFSPSDPRLRSADHPKALG"
     gene            complement(661..1470)
                     /locus_tag="PLS90_12840"
     CDS             complement(661..1470)
                     /locus_tag="PLS90_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPK03333.1"
                     /translation="MSPKPKRDEIDTYLKFAVQLAHEAGRITLRYYRQGVAVDLKADE
                     SPVTIADRDTEAFMRERIMEQFPDHGIIGEEHGRHNPDARWQWILDPIDGTQSFIRGI
                     PLYTVLLALECSGESHLGVIHCPAQDETVAAATGKGCTYNGEPCRVRATARLEEAWVN
                     VTNVADLARRRPRFTEALLRRAQSVRTWADAYSYLQVAAGRTDIAIDPVMNLWDIAPI
                     KPIIQEAGGRFSDFNGNSSIHTTNALVSTGPLHDELLALARLDEGDAQDPG"
     gene            complement(1568..3442)
                     /locus_tag="PLS90_12845"
     CDS             complement(1568..3442)
                     /locus_tag="PLS90_12845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK03334.1"
                     /translation="MSSPAPDSSLSPTQFRQTWRVVTWAGMFGAAYYTLCVTGAPRVK
                     FLTEIGASPFHFGLIATIGSLAILFQLFGGLLTNHVSRRKPVWMAVALTHRLIFAGVL
                     AAPLLFDSTGGRILCVMIVLALHDGIAQLGAPMWLTWMSDLVPADAMNRHWGGRQRVT
                     TLSIILTSILIVFAFDYFESSGRIILGFMLIGGLGIAVGVADVLLFLKVPEPPNEYDL
                     SRRFHEILLQPLLDRRYRAFVLFSAYYWFSVMVAWPFFTLYMVDFLGVSSRMTQLILA
                     AGPLGILLATRFWGLMCDNYGQRPVLQLTIIGKTLFPLAFILAPPNEAAAITILAMAM
                     FLDGLIDGGVLLALQGIMLKHSPRLNRSMYVAATQFFSLGVAGGIAPLLAGWGIDAMQ
                     GRTVQAGPYLLGGFHLAFLISLLMRLGAFYLAAKLNEPLAMPLGKLLRHMLTFKLLRV
                     SRLLWTLSEAEEADRRVEAARKLGELRSPLAIRDLIHALDDPAAAVREAAGDALTRIA
                     AAETTGPLAEALINPTSPIRPQAARLLGRIGDLDSMKALLTSLEDVDPAVLGEVVDAL
                     GQIGDWSAILPLIMLYDRVEDPDLSHRIVDAMMRLSESESEEETLALLSNSRPSAVRT
                     "
     gene            complement(3634..4125)
                     /locus_tag="PLS90_12850"
     CDS             complement(3634..4125)
                     /locus_tag="PLS90_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03335.1"
                     /translation="MNPMTQQTLRRAREIWHWALLSPLVYLLIAIGIDQAGWIDAEPL
                     APRPLDTPTIQGIILGLALAAVATLIFARVQQQRILRGAAGPAQPAVREHLERLLALW
                     RRRYYLALAMADALAFLGLIYFLISARMWALMAAGAAAYLAYAACYPLSGKARKRNGT
                     EGT"
     gene            4369..5220
                     /gene="modA"
                     /locus_tag="PLS90_12855"
     CDS             4369..5220
                     /gene="modA"
                     /locus_tag="PLS90_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPK03336.1"
                     /translation="MRQNQAEPCPPVLRRRNAGPLAGCLALLVVLALPWSGCAEASRE
                     DDLEEPITIFAAASTTEVLTEVLDALTTGSAVTCRTSFLASSTLARQIDQGAGADIFI
                     SANADWMDWLAERGRIQPDSRRDLLRNELVLIAPRDRVFKVRIAPEATFADDFTGRLA
                     LADPDHVPAGIYACQALEALGWWSRLEPRIVRAADVRDALRFVETAQADAGIVYRTDA
                     VASDRVAVVAAIPAELHDPIRYPVALTVRADPRAGELLDRLASPAAAAIFARYGFTPL
                     QSAASAP"
     gene            5217..5942
                     /gene="modB"
                     /locus_tag="PLS90_12860"
     CDS             5217..5942
                     /gene="modB"
                     /locus_tag="PLS90_12860"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019952773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPK03337.1"
                     /translation="MSWPPLTPLEWSALLLSLKVAGGCVLVSVVPGVALGWLLARRRF
                     PGKAVVDALVHAPLVIPPVVTGVLLLAVFGRRAPLGAWLEQTFGLSLAFTWQGAVLAS
                     AVMGFPLLVRAVRLAMELVDRRLEEAARTLGAGRWRTFFSITLPLALPGFLTGCVLAF
                     ARSLGEFGATITFAGSVAGETQTIPLAVYGTLQTPGAEAATLRLSLLSILLALAALLV
                     SEFLARRMRRRLGRRSGAEEGLP"
     gene            5939..>7024
                     /gene="modC"
                     /locus_tag="PLS90_12865"
     CDS             5939..>7024
                     /gene="modC"
                     /locus_tag="PLS90_12865"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HPK03338.1"
                     /translation="MKLAFDLTVRRPGFTLNARAEIDAGRTALFGPSGAGKTTLLLAL
                     VGLLRPDAGRIELDSETLFDAASNRWRPPERRGIGLVFQDDRLFPHMTVEENLLYGWR
                     RLRPAQRRFAPQSIVELLELGGLRGHYPAALSGGEARRVALGRALLASPRLLLLDEPL
                     TGLDLRLRRQILPFLRRIRDEAEIPIIQVSHDLEALLDLCEWFVLLDRGELVAQGRYL
                     DLLARPEALRLLHEPGLLNVLRLAVVRHDPDAGLSFLRFPGSAGEGDVAGEGEIRAPF
                     LDRPAGSLADLALRPQDIALARTAIAGISIQNQFAGRVERILESAGRVLCVVRIGAGV
                     DPARALIVEVTRHAVHEMALKPADTVWC"
BASE COUNT         1078 a         2223 c         2481 g         1242 t
ORIGIN      
        1 ggaaaaatca tattccggtc tcatctcttc tcgcgttgac gactcatcgg tcttaacctt
       61 cttcatagga tctcctctca cgcgccgcag ctctgcggca acttataata cgtattctgc
      121 cctgtctttc cgtgtgaatc aaaatcaaaa ccctctctct gcaagagagg ccaatgctga
      181 tgaagcgctc ctcttcctcc gaagagctaa tgtctggaaa ggtcaacaag aatggatcgt
      241 taaagatagt cttgccttcc tcaaagccca ctttatgctt cttcaggttt tcttttgcct
      301 ttgatctatc ccactcaaaa atcaggtcca tgaaatccct ccctcttatg cccctcagta
      361 tggtcaccca agcgccttag gatgatcagc ggagcggagc cgtgggtcgg acggcgagaa
      421 agcatcccgt acgggacgga cgtcttcggt gcgaagggta agcaatacct gagtgcgtcc
      481 agaggccgtg acgaactcga cttcaagggc cgcggtttcg tagacctgca taactgctcc
      541 aagatccccg gctttcagac ctgagaaggg gatgtcgtgg gacagcacga ccatgtcgaa
      601 ttccgatagg caattgacat ccgttcgctc cctgattagt agacaacttc attctttgtg
      661 ctacccgggg tcctgggcat ccccttcatc gagccgggcc agggcgagca gttcgtcgtg
      721 cagcgggccg gtgctcacca gagcgttggt cgtgtgaatt gatgaattgc cattgaagtc
      781 gctgaagcgg ccgccggcct cctggatgat cggtttgatc ggcgcgatgt cccacaggtt
      841 catcaccggg tcgatggcga tgtcggtccg tcccgccgcc acctgcaggt aggagtaggc
      901 gtcggcccag gtgcggacgc tctgggcgcg gcgcaggagc gcctcggtga atctggggcg
      961 gcgccgcgcc aggtcggcga cgttggtcac gttgacccac gcctcctcca gccgcgccgt
     1021 cgcgcgcacg cggcacggct cgccattata ggtgcagccc ttgcccgtcg ccgccgcgac
     1081 cgtctcgtcc tgggccgggc agtggatcac gcccaggtgc gactcgccgc tgcattccag
     1141 cgcgagcagc acggtgtaga gcgggatgcc gcggatgaag gactgcgttc catcgatcgg
     1201 atcgaggatc cactgccagc gggcgtcggg gttgtggcgg ccgtgctcct cgccgatgat
     1261 gccgtggtcg gggaattgct ccatgatccg ctcgcgcatg aacgcctcgg tgtcgcggtc
     1321 ggcgatggtg acggggcttt cgtcggcctt caaatcgacg gcaacgccct ggcggtaata
     1381 acgcagggtg atccggccgg cttcgtgcgc cagctggacg gcgaatttca ggtacgtgtc
     1441 gatttcgtcg cgtttgggtt tggggctcat gcgtggcggc tcctggggcg ggctatgaac
     1501 cgggcggttc acgtcaacat gtcggaaggt aggtgctggc aaggccaaaa tcaagccccg
     1561 gggggtctta cgtccgcacg gcgctggggc ggctgttgct gaggagggcc agcgtttcct
     1621 cctcggattc ggattcgctc aggcgcatca tcgcatcgac gatccggtgg ctcagatcgg
     1681 gatcctcgac gcggtcgtag agcatgatca gcgggaggat ggccgaccag tcgccgatct
     1741 ggccgagtgc atcgacgacc tcgccgagga cggccggatc gacgtcctcg aggctggtca
     1801 gcagggcctt catcgaatcg aggtcgccga tgcggcccaa cagccgcgcg gcctgggggc
     1861 ggatcggcga ggtggggttg atcaacgcct cggcgagggg gccggtcgtc tcggcggcgg
     1921 cgatccgggt gagggcatcg ccggccgcct cgcgcacggc ggccgcggga tcgtcgaggg
     1981 cgtggatcag atcgcggatc gccagcggcg agcgcagctc gcccagcttg cgcgccgcct
     2041 ccacgcgccg gtcggcttct tccgcctcgc tcagcgtcca cagcaggcgg ctgacgcgga
     2101 gcagtttgaa cgtcagcatg tggcgcagca gtttgcccag gggcatcgcc aggggttcgt
     2161 tcagtttcgc cgccaggtaa aatgccccca gccgcatcag caggctgatc aggaatgcca
     2221 ggtggaagcc gccgagaagg tagggccctg cctggacggt ccgtccctgc atcgcatcga
     2281 tgccccagcc ggcgagcagg ggggcgatgc cgccggccac gccgagagag aagaactgcg
     2341 tcgccgctac atacatcgaa cggttgagcc ggggcgagtg cttgagcatg atgccctgca
     2401 gagccagcag gacgccgccg tcgatcaggc cgtcgagaaa catcgccatg gcgaggatcg
     2461 tgatcgccgc cgcctcgttc ggcggcgcca ggatgaaggc cagcgggaag agggtcttgc
     2521 cgatgatcgt gagctggagc accgggcgct gaccatagtt gtcgcacatc aggccccaga
     2581 agcgggtggc aagcaggatc ccgagcggcc ccgccgccag gatgagctgg gtcatgcgcg
     2641 agctgacgcc gaggaaatcg accatataaa gcgtgaagaa aggccaggcg accatcaccg
     2701 agaaccagta ataggccgag aacagcacga atgcccggta acggcggtcg aggagcggct
     2761 ggagcaggat ctcgtggaac cggcggctca ggtcgtactc gttgggcggc tcggggactt
     2821 tcaggaagag gaggacgtcg gccacgccca ccgcgatccc caggccgccg atgagcatga
     2881 atcccaggat gatccggccg ctcgactcga aatagtcgaa ggcgaagacg atcagaatcg
     2941 acgtgaggat gatcgagagg gtggtgacgc gctggcgtcc gccccagtgg cggttcatgg
     3001 cgtcggcggg cacgaggtcg ctcatccagg tgagccacat cggggcgccg agctgggcga
     3061 tgccgtcgtg cagggccagc acgatcatca cgcagaggat gcggccgccg gtgctgtcga
     3121 acagcagcgg cgccgccagc actccggcga agatcagccg gtgggtcagc gcgacggcca
     3181 tccagaccgg cttgcggcgg ctgacatggt tggtcagcag gccgccgaag agctggaaca
     3241 ggatcgcgag ggagccgatg gttgcgatca ggccgaaatg aaagggactg gcgccgattt
     3301 ccgtgaggaa cttgacgcgc ggcgcgccgg tgacgcagag cgtgtaatag gccgcgccga
     3361 acatcccggc ccacgtgacg acgcgccagg tctggcggaa ctgggtgggc gagagtgaac
     3421 tgtccggcgc cggagacgac atattcccgc cattatcgca ggcgcgcaag gggcggcaag
     3481 gacaatcccg ggccgcactt cagcgacttg ctgcaggggg tggggcgctc acgaagaggc
     3541 atatcgctgc gcgacgtgcg gataggtggc gatcgcgata gcgatagcga tagcgatacc
     3601 gatggcgata tcgatgctcc cttggggcct cggtcaagtc ccttccgttc cgttccgctt
     3661 tcgggctttt ccgctgagtg ggtagcaggc cgcgtaggcc aggtaggctg ccgcgccggc
     3721 ggccatcagc gcccacatcc gggcggagat caggaagtaa atcaggccga ggaacgccag
     3781 cgcatcggcc atcgccaggg ccagataata ccgccggcgc cagagcgcca gcagccgctc
     3841 caggtgttcg cgcaccgcgg gctgggccgg gccggccgcc ccgcgcagga tccgctgctg
     3901 ctggacccgg gcgaaaatca gcgtggcgac cgccgcgagc gccaagccca ggatgatccc
     3961 ctggatcgtc ggcgtgtcga gcgggcgcgg cgccaacggt tcggcgtcga tccagcccgc
     4021 ctggtcgatg ccgatcgcga tcagcagata gaccaagggg gagaggagcg cccagtgcca
     4081 gatctcacgg gcgcggcgga gggtctgctg ggtcatcggg ttcatctcgg ctgagcgact
     4141 ccctcggggc gaactcccct ccaccctacc ggctggaaac gaaatcgcaa tgtctctcgc
     4201 ccaggatcgg cttcggcttg cgttccatgg catggatggt ttccgttagg gtgtccacct
     4261 ccgcgggcgg aggggctagc cggcggcgcg ttgctttcgc ggtcgcgttg ggttagaaac
     4321 gggcgaccgc caggccgcag gtccgggtta agaatggaag attcgcgtat gcgtcagaac
     4381 caggccgagc cctgccctcc agtcttgcgc cggcgaaatg ccggtccgct cgccgggtgc
     4441 cttgcgctgc tggtcgtcct ggcgctgccg tggtcgggtt gtgccgaggc aagccgtgag
     4501 gacgatctcg aagagccgat cacgattttc gccgccgcca gcacgacgga ggtcctgacg
     4561 gaggtgttgg acgcgctgac aaccggttcc gccgtgacct gccggaccag ttttctggcc
     4621 tcttccacgc tggcgcggca gatcgaccag ggggccgggg ccgacatctt tatctcggcg
     4681 aatgccgact ggatggattg gctggccgaa cgggggcgga tccagccgga ctcgcgccgc
     4741 gacctgctgc gcaacgagct ggtgctgatc gcgccgcgcg accgcgtgtt caaggtccgg
     4801 atcgcgccgg aggcgacgtt tgccgacgat ttcacgggcc ggctggcgct cgccgacccg
     4861 gaccacgttc cggccggcat ttacgcgtgc caggcgctgg aagcgctcgg ctggtggagt
     4921 cggctcgagc cgcggatcgt ccgcgccgcc gacgtgcgcg atgccctgcg gtttgtggaa
     4981 accgcgcagg ccgatgcggg catcgtctat cgcaccgacg ccgttgcctc cgatcgcgtc
     5041 gccgttgtgg ctgcgatccc cgccgagctc catgatccga ttcgctaccc cgtcgcattg
     5101 accgtcaggg ctgatccacg cgccggtgag ttgctcgacc ggctggcgtc gccggccgcc
     5161 gccgcgatct tcgcccgtta cgggtttacg ccgcttcaat cggccgcgtc cgcgccatga
     5221 gctggccgcc gctcacgccg ctggagtggt cggcgctcct gctgtcgctg aaggttgccg
     5281 gcggctgcgt gctcgtgtcg gtcgttcccg gcgtggcgct gggctggttg ctggcgcgcc
     5341 gccggtttcc cggcaaggcg gtggtcgacg cgctcgtaca cgcgccgctg gtgattccgc
     5401 ccgtcgtgac cggcgtgctg ctgctggcgg ttttcggtcg gcgcgcgccg ctgggggcgt
     5461 ggctggagca gacgttcggg ctctcgctgg cgttcacctg gcagggggcg gtgctcgcgt
     5521 cggcggtgat ggggtttccg ctgctcgtcc gcgccgttcg gctcgcgatg gaactggtcg
     5581 atcgccggct cgaggaggcc gcgcggacgc tgggcgccgg ccgctggcgc acctttttct
     5641 cgatcacgct gccgctcgcg ctgcccggct ttcttaccgg ctgcgtgctg gcctttgcgc
     5701 gcagcctcgg cgaattcggc gcgacgatta cgttcgccgg gagcgtcgcc ggcgagacgc
     5761 agacgattcc gctggctgtt tacggcacgc tgcagacgcc gggggccgag gcggcgacgc
     5821 tgcggctgag cctgctctcg atcctgctgg cgctggcggc cttgctcgtc tccgaatttc
     5881 tggcgcgccg gatgcgccgc cgcctgggcc gccgctccgg cgcggaggaa gggttgccgt
     5941 gaaactcgcg ttcgacctca ccgtccggcg cccggggttt acgctgaacg cgcgggcgga
     6001 gatcgacgcc ggccgcacgg cgttgttcgg cccctccggc gcgggcaaga cgacgctgct
     6061 gctcgcgctc gtcgggctcc tgcgccccga cgccgggcgg atcgaactgg acagcgaaac
     6121 cctgttcgat gccgcaagca accgctggcg tccgcccgag cgccggggga tcggtctcgt
     6181 ttttcaggac gaccgattgt tcccgcacat gacggtcgag gagaatctgc tttacgggtg
     6241 gcggcggctg cgtccggcgc agcgacgttt tgcgccgcaa tcgatcgtcg agctgcttga
     6301 attggggggc ctgcgcgggc actatccggc ggcgctatcg gggggcgagg cgcggcgggt
     6361 tgccctcggc cgcgcgttgc tggcgtcgcc gcggctgctc ttgctcgatg agccgttgac
     6421 cgggctggac ctccggctcc gccggcagat acttccgttc ctgcgccgga ttcgcgacga
     6481 ggccgagatc ccgatcatcc aggtcagtca tgatctcgaa gcgctgctcg acctgtgcga
     6541 atggttcgtt ctgctcgatc ggggcgagct ggtggcgcag gggcgctacc tggatctgct
     6601 ggcgcggccc gaggcgttgc ggctgctgca cgagccgggg ctgctcaacg tgctgcggct
     6661 ggcggttgtc cgtcatgatc cggacgcggg gctttcgttc ctgcggtttc ctggctccgc
     6721 cggcgaaggg gatgtcgccg gggaggggga gatccgcgcg ccgtttctgg atcggcccgc
     6781 gggctcgctg gccgacctcg cgctgcggcc gcaggatatc gcgctcgcgc gcacggccat
     6841 cgcggggatc tcgatccaga accagtttgc ggggcgcgtc gagcggattc tggagtcggc
     6901 cggccgcgtc ctctgcgtcg tccggatcgg cgccggcgtg gacccggcgc gcgcgctgat
     6961 cgtcgaggtg acgcgccacg ccgtgcatga gatggccttg aaacccgccg acacggtctg
     7021 gtgc
//
LOCUS       DAOPCO010000208         6987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9665_length_6987_cov_4.062897, whole genome shotgun sequence.
ACCESSION   DAOPCO010000208 DAOPCO010000000
VERSION     DAOPCO010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9665_length_6987_cov_4.062897"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1279
                     /locus_tag="PLS90_12870"
     CDS             242..1279
                     /locus_tag="PLS90_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPK03339.1"
                     /translation="MSVKYKDYYKLLGVERTASQDEIRKAFRKLAAKYHPDRHQGDKA
                     MEEKFKEVNEAYEVLRDPTKRQRYDALGANWKQGQQFDPNAFNNIFGGRATTGRTGGF
                     GGAQTFRMETGPGGFSEFFEALFGGMGVGGMGSGRGGGVEDLFARFEEGPARRGGAEE
                     FGGGGRPSVNVEASITISLEEAYKGGVRTISFRRTEPNGQASTQGYEIRIPPGIRDGQ
                     KLRLKGQGSVAGAAAGDILIVVKVRPHPRFTLEGNDLITDLPLAPWEAVLGARIEVPT
                     LDGSVEVKVPAGMQSGKRMRVKGAGWPGKGGQKGDLYLRVTIQVPENPGAQEKELYER
                     LGRVSRFDPRK"
     gene            1404..2069
                     /locus_tag="PLS90_12875"
     CDS             1404..2069
                     /locus_tag="PLS90_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03340.1"
                     /translation="MRSNALIGRLRRVAGALALTVVLLTGCSVVEVHSYTDPKMEVRR
                     EGAPRVCVVTVDMRPYIVSGDFPPSFTGIVHGFWGNANAVLTASGQPLAEEMSRAVAG
                     GLRREGLDPVILTVPAGTSPDVIQRRLQECGAERTVTLRVNEWKTRTWMRTSATYDLD
                     WYFRETTPTGKQEASHEHSGRENLGGNLGDPRGYARVAVQRFFKRTIDEALADPNVKR
                     VLE"
     gene            2871..3179
                     /locus_tag="PLS90_12880"
     CDS             2871..3179
                     /locus_tag="PLS90_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPK03341.1"
                     /translation="MNIYVGNLSYQTTESGLRAAFEEHGEVSSAAIIKDAETGRSKGF
                     GFVEMSDSEQAKSAISALDGSDLDGRQIKVNEARPRGDRPAGGPRGGGNRNFQRRERW
                     "
     gene            complement(3326..4225)
                     /locus_tag="PLS90_12885"
     CDS             complement(3326..4225)
                     /locus_tag="PLS90_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPK03342.1"
                     /translation="MAIGYLELLNRFEEFLRVERNLSPRTRKAYLYDLTRFSEFLILG
                     HGRMPSLSLISTDVIREYLNHLQLEHACKSTTLARTISSIRAFFQFAVEREVLEASPA
                     ARIRTPKQPKKLPIYLVPQELVRLLEAPAADTWSGLRDRAILATLAFTGVRLSEVTGI
                     NLPDIDLTNRAVRVLGKGNKERIIPLNEIVCEAINRYLNVRPLSEAQALFLNKFGRRL
                     SGRSVENIVRKYALKAGIFRDGVSPHKLRHTFATLLHANEVDLIEIKSLMGHANIAST
                     QIYTHTSNSRLRAAVQKIENLPL"
     gene            complement(4507..5922)
                     /gene="mnmE"
                     /locus_tag="PLS90_12890"
     CDS             complement(4507..5922)
                     /gene="mnmE"
                     /locus_tag="PLS90_12890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPK03343.1"
                     /translation="MPPAGLSTTDPPGERRPPARDELIVARATPPGAAALAVVRIDGA
                     GARDIVQRLFVPAGRRSPIDTPRTMIFGHWRDPLSGDRLDEGLTVFFPAPHSYTGNDL
                     AEFHGHGGAVAPRRLVEAVLACGARLAEPGEFTRRAFLNGRLDLAQAEAVADLISAQT
                     DAAARIAQRQLVGELSSRIHALRDDLLTLAAEIEARIDFPEEDLDPADQSRLAAAFAA
                     ALVDLRTLAETRRRGRLLREGARVALIGRPNAGKSSLLNALVRMERAIVTPHPGTTRD
                     VIECTLDLHGIPVTLVDTAGLRESHDPIEQLGIERTRREIERADLVLRVVDLTESPAP
                     AIDAELSDGGRPADITVFNKLDLAPDHAPPCQGDACSVSATRGDGMADLERLLVARLD
                     AAGAGETESGFAVNLRQGELLDRARAALHAARAAFDQGLSGEFVMVDLREALDALSAI
                     LGLEVGDAVLDRIFSRFCIGK"
     gene            6073..6669
                     /locus_tag="PLS90_12895"
     CDS             6073..6669
                     /locus_tag="PLS90_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008101660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPK03344.1"
                     /translation="MEERLERQLAFVHEIDKLKRIERRNYLTDGSRRENSAEHSWHLA
                     LMALVLAEWSNEERIDLGRVLPMLLLHDLVEIDAGDTFVYDEAAHADKAAREEAAADR
                     IFGLLPEEQGRRLRALWEEFESGQSAEAQFARALDRLQPIMMNFASGGKPWVDHRITR
                     RQVVGRNQHIEQGSRRLWDVARGIIERACKAGLLADAK"
BASE COUNT         1219 a         1842 c         2837 g         1089 t
ORIGIN      
        1 gccgacgctg ctggacctgc tggggatcga cccgccggag gaaatgactg gcaaatcgct
       61 gatcgtccgc taacgtgaga aggggccgcg ccgggatgaa agcgatgcgg gcaacctggg
      121 catccggttg gttttgtgct aaactgggtt gcggccgagg gtcgcgcccg cgcgtcgcgg
      181 ccggcggccg ggccaacgcc gggcggcggc gggaagtccc cgttcgtggt gacgaagcgc
      241 gatgagcgtc aaatacaaag actactacaa attgctcggc gtggagcgca ccgcttcgca
      301 ggatgagatc cgcaaggcgt tccgcaagct ggccgcgaag taccatcccg atcgccatca
      361 gggcgacaag gcgatggagg agaagttcaa ggaagtcaac gaggcctacg aggtgctgcg
      421 cgacccgacc aagcgccagc gctacgacgc gctgggcgcc aactggaagc aggggcagca
      481 gttcgacccc aatgcgttca acaacatctt cggcggccgg gccaccaccg gccggacggg
      541 ggggttcggc ggcgcgcaga cgttccggat ggagaccggg ccgggcgggt tcagcgagtt
      601 cttcgaggcg ctgttcggcg gaatgggcgt cggggggatg gggagcgggc ggggcggcgg
      661 cgtggaggat ctgttcgccc ggtttgaaga gggccccgcg cggcggggag gggcggagga
      721 attcggcggc ggcggccggc ccagcgtgaa tgtcgaggcg agcatcacga tttcgctgga
      781 ggaagcctac aaggggggcg tgcggacgat cagcttccgc cggaccgagc cgaacgggca
      841 ggcgtcgacg cagggttacg agattcggat tccgcccggc atccgcgatg gccaaaagct
      901 gcggctcaag ggacagggga gcgtagcggg ggcggcggcg ggcgatattc tgattgtggt
      961 gaaggtgcgg ccgcacccgc gattcacgct ggaagggaac gatttgatta cggatctgcc
     1021 gctggcgccg tgggaagcgg tgctgggggc gcggatcgag gtgccgacgc tggatggctc
     1081 ggtggaggtg aaggtgccgg cggggatgca gagcggcaag cggatgcggg tgaagggggc
     1141 gggttggccc gggaaggggg ggcagaaggg agatctttac ctgcgggtca cgatccaggt
     1201 gccggagaat cccggcgcgc aggagaagga gttgtatgag cggctggggc gggtctcgcg
     1261 gtttgacccg cggaagtaag gggggaggga gagaagagga attcgcgagc cgaggacgag
     1321 agccgagagc cgaattctgg tttagagcgg gggacgaggg acgagttttg gagatgcgaa
     1381 cgataccggg aaggaaagaa tccatgagat ccaatgcgtt gatcggacgg ctgcgccggg
     1441 ttgccggcgc gctggcgctg accgtcgtgc tgctgaccgg ctgttcggtg gtggaggtgc
     1501 actcctacac cgatcccaag atggaggtgc ggcgcgaagg agcgccgcgg gtctgcgtcg
     1561 tgacggtcga catgcggccc tatattgtgt cgggggattt tccgcccagt ttcacgggga
     1621 tcgtgcatgg gttctggggg aacgccaatg cggtgctgac ggcgtcgggc cagccgctgg
     1681 ccgaggaaat gagccgggcc gttgcggggg gcctgcgccg cgaggggctg gatccggtca
     1741 tcctgacggt gccggccggg acgtcgcccg acgtaatcca gcggcggctg caggagtgcg
     1801 gcgcggaacg gaccgtgacg ctgcgcgtca acgagtggaa gacgcggacg tggatgcgga
     1861 catcggcgac gtatgatctg gactggtact tccgggagac gacgccgacc ggcaagcagg
     1921 aggcgtcgca tgagcacagc gggcgggaga atctgggggg caacctgggc gatccgcggg
     1981 ggtatgcgcg ggtggccgtg cagcggtttt tcaagcggac gatcgacgag gcgctggccg
     2041 atcccaatgt gaagcgggtg ctggagtagg gctgggacct ggttatgttt cgggggccgt
     2101 ccgggcaccc cttgcagggg tttggcgcgt tgggggtggc gacacagggt tccggcctgc
     2161 ggccgtcacc ctgtgcttta ttccggtcgt ccgctgcgcg gactgaaggg ttcgccccgt
     2221 tcggggacgg ggaggatttg gggatcagcg ggccccaggt tgcgctgcgc tccaccaggg
     2281 gcaaatcaaa tttcgccgct tcgaggcgac agccggcatc cctccgggat gcgggggaat
     2341 gggggtccgg ggggaccggt ggtgtcgctg cgctcaacca ccggctaccg gctggcatcc
     2401 ctcccgggat gcgggggctg gctcatcgcg taacgctcga ccaactgctc atcctggcat
     2461 ccttgcggga tgcgggggaa cgcgatccgg gggcggcgag ccacagtggt ggcgcttcag
     2521 ttgctgtcct gccgcgcaag gggtcttttt tcgagagccg aggacgaggg acgggagccg
     2581 agagccgaac gaatggatgg ggagggtccc ggacggtgac tccccagttg ggacggccag
     2641 gcggtgacgg cccggggggg aagggtgtcg gaggggtgaa aaaattaacg gttgggcccg
     2701 cgaaatcccg attgacaggg aagggggtgg aaacgtagag tatcggcatg cctgccactc
     2761 gcgttactcc ccttcggtaa attcgaatgg ttacggtaga tatcgaacaa gggaaggcct
     2821 gcgtcaatcg gatgcagctt gccttggcgt agaacggatt ggagaatcgt atgaacattt
     2881 acgtgggaaa cctttcctac cagacaaccg aaagcggact gcgcgccgcg ttcgaggagc
     2941 acggagaggt ttcgtccgcg gccatcatta aggacgcgga aaccggtcgc tccaagggct
     3001 tcggctttgt ggagatgtcc gacagtgaac aggccaaatc cgccatcagc gcgcttgatg
     3061 ggagcgattt ggatgggcgt caaatcaaag tgaacgaagc gcgtccgcgc ggtgatcgtc
     3121 ctgcgggcgg tcctcgcggc ggcggcaacc ggaatttcca gcggcgcgaa cgctggtaaa
     3181 ctcagcggca acgcgaagtc aggtccaaca acaagctttt gaatgaaacg atcggggtcg
     3241 gagccagaag ggggctccga ccccggttgc tttttgaggg gggatgaggg tgtgggcggg
     3301 aaaggcgatc aggtgggtct aaaggtcaga gcgggaggtt ttcgattttc tggacggcgg
     3361 cgcgcaggcg cgagttggag gtgtgggtgt agatctgcgt cgaggcgatg ttggcgtggc
     3421 ccatcaggga tttgatctcg atcaggtcga cctcgttggc gtggagcagc gtcgcgaacg
     3481 tgtggcgcag tttgtgcggg ctgacgccgt cgcggaagat gccggccttc agggcgtatt
     3541 tgcgcacgat gttctcgacc gagcggccgc tcagccggcg gccgaacttg ttgagaaaca
     3601 gcgcctgggc ttcgcttaac gggcgcacgt tcaagtaacg gttgatcgct tcgcagacga
     3661 tttcgttgag cggaatgatc cgttccttgt tgcccttgcc gaggacgcgg acggcgcggt
     3721 tggtcaggtc gatgtcgggc aggttgatcc cggtcacttc ggagagccgg acgccggtga
     3781 aggcgagcgt ggcgaggatc gcgcggtcgc gcaggccgga ccaggtgtcg gcggcgggcg
     3841 cctcgagcag ccgcaccagt tcctgcggga caaggtagat cggcagtttt ttgggctgct
     3901 tgggggtgcg gatgcgcgcg gccggggagg cctcgaggac ctcgcgttcg acggcgaact
     3961 ggaagaaggc gcggatggag gagatcgtgc gggcaagcgt cgtcgacttg cacgcgtgct
     4021 cgagttggag gtggttgagg tattcgcgaa tgacgtcggt ggagatgagc gaaagcgagg
     4081 gcatgcggcc gtggccgagg atcaggaatt cgctgaagcg ggtcaggtcg tagagatagg
     4141 ccttgcgggt gcggggggag agattgcgct cgacacgcag gaactcctcg aaacggttga
     4201 gcaactccag gtaaccaatc gccatcagcg gggactcgcg gaacggggtc aggcgcaacg
     4261 ggacgcggcg acgtgatgta cgagggcatc ttagaccaga agggggccgg gctgcacgtg
     4321 gttttgcgcg ggcgaggacg agttgctcag aaacccgcgg tttacaacaa ggggacaaaa
     4381 tgacgaggtc tcgagagccg aggacgaggg acgcgagccg agagccgaat tttggggagg
     4441 gcctatggag gcgactacga ggacgaggac gagggacgat gttggagcga ggacgagagc
     4501 agggggttac ttgccgatgc agaagcggct gaagatgcgg tcgaggacgg cgtcgccgac
     4561 ttcgaggccg aggatcgcgc tcagggcatc gagcgcctcg cgcaggtcga ccatcacgaa
     4621 ctcgcccgac aggccctgat cgaatgcggc gcgggcggca tgcagcgcgg cgcgggcgcg
     4681 gtcgagcaac tcgccctgac gcagattgac ggcgaaaccc gattcggtct cccctgcccc
     4741 ggcggcgtcg agacgcgcga ccaacaggcg ctccaagtcg gccatgccgt cgccgcgcgt
     4801 ggccgagacg gagcaggcat cgccctggca aggcggcgcg tggtcgggcg cgagatcgag
     4861 tttgttgaag acggtgatgt cggcggggcg accgccgtcg gacagttcgg cgtcgatcgc
     4921 cggcgcgggg ctttcggtca ggtcgaccac gcggaggacc agatcggcgc gctcgatctc
     4981 gcggcgggtc cgttcgatgc cgagctgttc gatcggatcg tggctttcgc gcaggccggc
     5041 cgtatcgacg agcgtgacgg ggatgccgtg cagatcgagg gtgcattcga tgacgtcgcg
     5101 cgtcgtgccg gggtgggggg tgacgatggc gcgctccatg cggacgaggg cgttgaggag
     5161 gctggatttg ccagcgttgg ggcggccgat gagggcgacg cgggctcctt cgcgcaggag
     5221 ccggccgcgg cggcgcgttt cggccaaggt gcggaggtcg accagcgccg cggcaaacgc
     5281 ggcggccagg cggctctgat cggccgggtc gaggtcctct tcggggaaat cgatgcgggc
     5341 ctcgatttcg gcggcgaggg tcagcaggtc gtcgcgcagc gcatggatgc gcgaagagag
     5401 ttcaccgaca agctggcgct gggcgatgcg cgcggcggcg tcggtctggg cgctgatcag
     5461 gtcggcgacc gcctcggcct gggccagatc gagccggccg ttgagaaaag cgcggcgggt
     5521 gaactcgccc ggttcggcga ggcgggcgcc gcaggcgagc acggcctcca ccaggcggcg
     5581 gggggcgacc gcgccgccgt ggccgtggaa ctcggccagg tcgttgccgg tgtaggaatg
     5641 cggagcgggg aagaagacag tcaggccctc atcgaggcgg tcgcccgaca gcggatcgcg
     5701 ccaatggccg aagatcatcg tgcggggggt gtcgatcggc gagcggcggc cggcgggaac
     5761 gaagaggcgc tggacgatat cgcgggcgcc ggcgccgtcg atgcggacga cggcgagggc
     5821 ggcggcgccc ggcggcgtgg cgcgcgcgac aatcagttcg tcgcgggcgg gggggcggcg
     5881 ttcacccggc gggtcggtgg tgctgagtcc ggcggggggc atcacggatg ggcggaatcc
     5941 cttcgggcag ttgcttcggg cgttggtcgc ccttatcgta gcataagccc gcgcgccgcg
     6001 tcgcggggag ggaggcgggc gatggccggg gttgcgcgtc ggacctcgca gtttgaatac
     6061 gggagagaag ccatggaaga gcgtctggag cggcagcttg cctttgtcca cgagatcgac
     6121 aagctgaagc ggatcgagcg gcgcaattac ctgaccgacg ggtcgcggcg ggagaacagc
     6181 gccgagcatt cgtggcatct ggcgctgatg gcgctcgtgc tggcggaatg gtcgaatgag
     6241 gagcggatcg acctgggacg cgtgctgccg atgctgctgc tgcatgatct ggtcgagatc
     6301 gatgccggcg atacgttcgt ctacgacgaa gcggcgcatg cggacaaggc ggcgcgcgag
     6361 gaggccgccg ccgaccggat cttcgggctg ctgccggagg agcagggccg gcggctgcgc
     6421 gcgctgtggg aggagttcga gtcggggcag agtgcggaag cgcagttcgc ccgggcgctc
     6481 gaccggctgc agccgatcat gatgaatttc gcttcggggg ggaagccgtg ggtcgatcac
     6541 cggatcacgc ggcggcaggt ggtcgggcgc aaccagcaca tcgagcaggg ctcgcggcgg
     6601 ctgtgggacg tcgcgcgggg gatcattgaa cgggcgtgca aggcggggct gctggcggat
     6661 gcgaagtgag gggggagtaa gacacgggat cacgaaaggc cgaaggggca cgagagacag
     6721 gaaagggatg ggggtgagga tggggatggg gatgcggatg ggaaggaacc ggcgagggcg
     6781 ccggtcccac aaccccacaa ctggggatcg catttcggtg gcggtgctgc caggcagaag
     6841 ggccggtttt cgagagccga ggacgaggga cgagagccga gagccgaatt tgggggaggc
     6901 ctttgggggc aatgccactc agagcgtggc gggcccaggt ttcggccggt tgcttgtttc
     6961 catgcggcgt cgaggcagga ggggggc
//
LOCUS       DAOPCO010000209         6975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9679_length_6975_cov_4.540462, whole genome shotgun sequence.
ACCESSION   DAOPCO010000209 DAOPCO010000000
VERSION     DAOPCO010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9679_length_6975_cov_4.540462"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLS90_12900"
     CDS             complement(<1..1443)
                     /locus_tag="PLS90_12900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03345.1"
                     /translation="MTTALTAASARPFEILPRVESGIDLTAGQAAARLGALYGVFVII
                     RTLMNQTVQGLTVYSDEALYMEAARTILQGNGTWWDAAFSGLPNYLYPLLIAWPITHF
                     PWETGFDLARSFNALAAGLTIFPSYALARGFVSRRRSLMVATLIATVPGMAYTMKLMT
                     ENVFLPLATLFMWLAWRAIVRPTAGRCLVAGLAAGLAFHAKPHALLLPPIYAGAAILF
                     EWIRWRTGRDEEGSRPTTGRELVRGIGLHGFAALGLFAALLPRLAEVIWLEQWATPFS
                     MRSFMGFYHYGVDSGQSGLNWPMFWQALCMNGGTWWVGAAILPAVALGRDARAVFGGS
                     APTRERALWLLAVLAGIAIVLTCARHVATFEREPTAYERYIYPFLTPVLLYFLFRVAS
                     QERPGMAVLISRSGRLIAIALGLAFGVFATWRLEGGWSYPSNTPTLSIGLLIARWRDV
                     SPLLIGGWGVLAGAGVLVMVGAPRGWRLNWW"
     gene            1687..2760
                     /locus_tag="PLS90_12905"
     CDS             1687..2760
                     /locus_tag="PLS90_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPK03346.1"
                     /translation="MPSHLAINHLTIDSFPQLDMIREIFGLDAPRIRHLPWGHGAQLL
                     VEDGHDAWILKRVRNPRPADQLEQILQAVRAVGAAAAGPLIPELRGTREGRVLYSSTQ
                     GHFYLMRKVSGRRPSFKRSGDTLQVLQALARFHAASRRAAPDLAAGLGLAPSVDLLAF
                     TEARAAAMRRLNRRVRRGHAAFLTPLGRWAAARHEPARRLARRAREFCRRTGREASAE
                     PAGGRLACPILLAHGDTHENNFLLDGDGAWLLDLESCTPRPVVLDLLVPLHTFAQANQ
                     WPADTLLDLVAQYEAIRPLEPAERILLGSQLITPRLWLRAMHSLVKRDGRFKWSTARA
                     LWRASRAMKPHRRFARALFDHWP"
     gene            3009..3656
                     /locus_tag="PLS90_12910"
     CDS             3009..3656
                     /locus_tag="PLS90_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03347.1"
                     /translation="MSPLRRLTLLLLTVVAIAFVAPTVSAQYETDPPTGYMPKDEPAP
                     DPPASAGRRVLEAICWYLPNRIVDIADIFHLYLTAGDGLGITVRATQLLWASWFEDDA
                     YGIGWDPRKPPIFGESVSERYFGFLFASQGDIDRDPTEIGLSLHFVYLGLNAAVSLGE
                     AVDALLGFAGIDLMADDHGPMLFESAEPAPEPAAQPTALWIEQPARDPGRAVSMP"
     gene            3824..4321
                     /locus_tag="PLS90_12915"
     CDS             3824..4321
                     /locus_tag="PLS90_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPK03348.1"
                     /translation="MTAKGGNDWRRSLDRLRDDFEQQLGQITRRLKSFDPVEAGGALA
                     SVFGRPLAAAVDMEEDDEAIRVRAEMPGVDPDTLKVEVQGQRLKLCGEKRTARHESQR
                     DCHYAECSYGLVSRSVMLPCEVNADQAEADYRDGVLTLRLPKPEGAKPRPIRVNVARS
                     TPPEG"
     gene            4628..5824
                     /gene="sat"
                     /locus_tag="PLS90_12920"
                     /pseudo
     CDS             4628..5824
                     /gene="sat"
                     /locus_tag="PLS90_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011243529.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
     gene            5927..6445
                     /gene="cysC"
                     /locus_tag="PLS90_12925"
                     /pseudo
     CDS             5927..6445
                     /gene="cysC"
                     /locus_tag="PLS90_12925"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
     gene            6571..>6975
                     /locus_tag="PLS90_12930"
     CDS             6571..>6975
                     /locus_tag="PLS90_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HPK03349.1"
                     /translation="MAAASTQHHEFPDLRGRHPREILEWAWATYGDRLMTTTAFGYSG
                     MALFHMMAQVAPRVPIYFIDTGYHFAETLQFRDYCRDKLGLNIIDLRPEKTREQFEAE
                     HGGPIYKTDPDRCCAINKVAPLQKMLRESNYAA"
BASE COUNT         1228 a         2753 c         1901 g         1093 t
ORIGIN      
        1 ccaccagttc agccgccacc cccgtggcgc cccgaccatc accagcacgc ccgccccggc
       61 cagcacgccc cacccgccga tcagcagcgg gctcacgtcg cgccagcggg cgatcagcag
      121 cccgatcgac agcgtcggcg tattggacgg gtagctccac cccccctcca gccgccaggt
      181 cgcaaacacg ccgaatgcca gccccagcgc gatggcgatc agccggccgc tccggctgat
      241 caggacggcc atccccggcc gttcctgact cgccacgcgg aacagaaaat acagcagcac
      301 cggcgtgaga aacggataga tgtagcgctc gtacgccgtc ggttcccgct cgaacgtcgc
      361 cacatgccgc gcacaggtca gcacgatcgc gatccccgcc agcaccgcca gcagccacag
      421 cgcccgttcg cgcgtcgggg ccgacccgcc gaacaccgcg cgcgcatccc gccccagcgc
      481 cacggccggc agaatcgccg ccccgaccca ccacgtcccg ccattcatgc acagcgcctg
      541 ccagaacatc ggccagttca gccccgactg cccgctgtcg accccataat gatagaagcc
      601 catgaagctg cgcatcgaaa acggcgtcgc ccactgctcc agccagatca cctcggccag
      661 ccgcggcagc agcgcggcga ataaccccag cgcggcgaac ccatgcagcc cgatcccgcg
      721 caccagttcg cgacccgtcg tcggccgcga cccctcctcg tcgcgtcccg tgcgccagcg
      781 gatccattca aacaggatcg ccgcccccgc ataaatcggc ggcagcagca acgcatgcgg
      841 cttggcgtgg aacgccagcc ccgcggccag gccggccacc aggcaccgcc ccgccgtcgg
      901 acgcacgatc gcccgccagg ccagccacat gaacaacgtc gccaggggca ggaacacgtt
      961 ctccgtcatc agcttcatcg tgtaagccat ccccggcacc gtcgcgatca gcgtcgcgac
     1021 cattaggctg cgtcgccggc tgacaaagcc gcgcgccagc gcgtaactcg gaaaaatcgt
     1081 caaccccgcg gccagcgcat tgaacgaccg cgccagatca aaccccgtct cccacggaaa
     1141 atgcgtaatc ggccacgcga tcagcagcgg atagagataa ttcggcaggc cgctgaacgc
     1201 cgcatcccac cacgtcccgt tgccctgcag gatcgtccgg gcggcttcca tgtagagcgc
     1261 ttcatcgctg tagaccgtca gcccctgcac cgtctggttc atcagcgtgc ggatgatcac
     1321 gaacacgcca tagagcgccc ccagccgcgc cgcagcctgt cccgccgtca ggtcgatccc
     1381 cgattccacc cggggcagaa tctcgaacgg gcgggcggaa gctgcggtca acgcggtcgt
     1441 catcggttcc attcgcggtt caaaagtcgc catatggtgc tggcccctcc ccgcaaaaat
     1501 caaggcgaaa ccccccatca caatcgattt cggtatcgct atcgctatcg ccatcgctag
     1561 cgatcttctg cccgcgaagc ggacatcaaa ccgatccctc cgctccagcc cgccccattt
     1621 ccccttgaac ctgcacgccg ctcaggatgc aatagaccgc cgccgcgcat cgatttgacg
     1681 ggggtcgtgc cgtcccactt ggccatcaat cacctgacca tcgattcctt cccgcaactc
     1741 gacatgatcc gcgagatctt cgggctggac gccccccgca tccgccatct gccgtggggc
     1801 catggcgcgc agctcctcgt cgaggatggc cacgacgcgt ggattctcaa gcgcgtgcgc
     1861 aacccgcgcc ccgccgacca gctcgagcaa atcctgcaag ccgtccgcgc ggtcggcgcg
     1921 gcggccgccg gcccgctgat ccccgagctg cgcggcaccc gcgaaggccg cgtgctctat
     1981 tcctcgaccc aggggcattt ctacctgatg cgcaaggtct cgggccgccg cccctcgttc
     2041 aaacggtccg gtgacacctt gcaggtgctc caggccctgg cgcggttcca cgccgcatcg
     2101 cgccgcgcgg cgcccgatct ggccgccggc ctcggcctgg ccccatccgt tgatctcctg
     2161 gccttcaccg aagcccgcgc cgccgctatg cgccgcttga accgtcgcgt ccgccggggg
     2221 cacgcggcgt tcctgacccc gctgggccgc tgggccgccg cgcggcacga acccgcccgc
     2281 cggctcgccc gccgggcgcg cgaattctgt cgccgcaccg gccgcgaagc gtccgccgaa
     2341 ccggccggtg gccggctcgc gtgcccgatc ctgctggccc acggcgacac ccatgagaac
     2401 aatttcctgc tcgacggcga cggcgcatgg ctcctcgatc tggaatcctg caccccgcgc
     2461 ccggtcgtcc tcgatctgct cgtcccgctc cacacattcg cgcaggccaa ccagtggccg
     2521 gccgacacac tgctggatct cgtcgcccaa tatgaagcca tccgcccgct ggagccggcc
     2581 gagcggatcc tgctcggctc gcaactcatc accccgcgtc tctggctgcg cgcgatgcac
     2641 agcctcgtca agcgcgacgg ccgcttcaaa tggtcgacgg cccgcgccct gtggcgcgcc
     2701 tcacgcgcga tgaaacccca ccgccgtttc gcccgcgcgc tcttcgatca ttggccctga
     2761 cccccaattt tcgtccctcc ccccacctcc tgcgcgagca ggacgccgcg catccccgaa
     2821 cagcccgatt gaagcccgcg ccccccgctg ccgataagat tccccggacg gttatgaccg
     2881 gcgtgcctgg agtccccacc gcgggtctca ttgaattgac tttccgcgct gtctgccgtc
     2941 tattgagagc agttcgcctc gattccgttg attccggcgg ccccgcgccg cccaaggagc
     3001 cgccctccat gtccccgctg cgccgcctga ccctgttgtt gctgactgtt gtggcgattg
     3061 cctttgtcgc gccgaccgtc tcggcgcaat acgaaaccga cccccccacc ggctacatgc
     3121 ccaaggacga gcccgccccc gacccccccg cctccgccgg acggcgcgtc ctcgaggcga
     3181 tctgctggta tctccccaac cgcatcgtcg acatcgccga catcttccac ctctatctga
     3241 cggcgggcga cggcctcggc atcaccgtcc gcgccaccca gctcctgtgg gcctcctggt
     3301 tcgaggacga cgcctacggc atcggctggg acccccgcaa acccccgata ttcggcgaaa
     3361 gcgtcagcga gcgctacttc ggcttcctct tcgccagcca gggcgacatc gatcgcgatc
     3421 cgaccgaaat cggcctctcg ctccatttcg tctatctcgg cctcaacgcc gccgtcagcc
     3481 tgggcgaagc ggtcgacgct ctcctgggct tcgccggcat cgacctgatg gccgacgatc
     3541 acggcccgat gctcttcgaa tcggccgaac cggcccccga acccgccgcc cagccgaccg
     3601 ccctctggat cgaacagccc gcccgcgacc ccggccgcgc cgtctcgatg ccgtaacgca
     3661 ccacccgcct gtgcgcgtgc gcaccgctgc gcgggcacgc agacgcctcg ccggccccgc
     3721 ccccctcatc tgactggctt ccaaccattg cccgcccgcc cggtgggtat gcctcttgca
     3781 tggaaaccct cacgccatgt gatcccctgc gaggaggtgt gtcatgaccg caaaaggtgg
     3841 caatgattgg cgacgctcgc tcgatcggct gcgcgacgat tttgagcagc aactcggaca
     3901 aattacccga cgcctcaaaa gcttcgaccc ggtcgaagcc ggcggtgcgc tcgcttccgt
     3961 cttcggtcgg cccctggccg ccgccgtcga tatggaagag gacgacgagg cgatccgcgt
     4021 ccgcgccgaa atgcccggtg tcgatcccga cacgctcaag gtcgaagtcc agggtcaacg
     4081 cctgaaattg tgcggcgaaa aacgcaccgc gcggcatgaa tcccagcgcg actgccacta
     4141 cgccgagtgc agctatgggc tcgtttcgcg ctcggtcatg ctcccctgcg aagtcaatgc
     4201 cgaccaggcc gaggccgact accgcgacgg cgtcctgacg ctgcgactgc ccaaacccga
     4261 gggcgccaag ccgcgcccga tccgggtcaa cgtcgcccgt tcgacgcccc ccgaaggttg
     4321 aaccgacacg cggacggggc gccccacccc cgtccgcccc aaatctcctc cctcgacttc
     4381 gtcactcgtc ctcgcccccg tcggcccacc tcataattcg gcttcggccc ccgtccctcg
     4441 tcctcgtcct cacccctcat catcatcccc acgtccaatc tccacatttc cgatcaattt
     4501 ggttgactat cccctccccc gccccgtaaa ctatctccgg ctttgacttg tccgacctga
     4561 tctgattgga tgtggcggat tggtttccct caagtccctg attgaaccca cccctcagga
     4621 gtttctgatg acgacgcttg ccgagccgac gactgtttcc gggatcgccc cccatggcgg
     4681 ccgactgatc aacctgctgc tggaaggggc cgacgccgat cgcgtcgagc gcgaagccca
     4741 agccctgcct caactcgaat tgaacgaacg tcaagtcaat gacctggaca tgctcaccgt
     4801 cggcgccctc tcccccctcg aaggcttcat gggccaggcc gattaccgct cggtgatcga
     4861 atcgacccgc ctcgccaacg gcctcgtctg gtccatcccc gtcgtcctgg gcgtcaccga
     4921 agcgcagaaa aatcagatcg aatcggccgg cgccgtcgcc ctcgtcgatc agggccgcat
     4981 cctcgccgtc ctgcgcgtca gcgatatctt ccagcccgac ctccccgccg aggcccgcag
     5041 cgtctacggc accaccgagc aggaacatcc cggcgtcgcg gcgattcacg cccgcggccc
     5101 ctggctgatc ggcggccgcg tcgaggcgat cgcccgccac gaatacgagg atttccccga
     5161 tagccgcctc accccggccg aaacgcgcgc cgtcttcgcc gagcgcggct ggaagcgcat
     5221 cgtcgccttc cagacccgca acccgatcca tcgcgcccat gaatacctcc tgcgctgcgc
     5281 cctggagatg gccgacggcc tcatggtcca ccccctgatg ggcgccacca agaagggcga
     5341 catccccggc gacgtgcgct ggcgctgtta ccagaccctg ctcgagaaat atttcccccc
     5401 cgagcgcgtc ctgctctcga tcttcccggc caacatgaat tacgcgggac ccaaggaggc
     5461 gatctaccac gcgatctgcc gcaagaacta cggtgtgacc catttcatcg tcggccgcga
     5521 ccacgccggc gtcggcaagt actacggcac ctacgatgcg caggaaatct tcgacaactt
     5581 ctcccgcgag gagatcggca tcgaaatcct ccgcttcgag cactccttct ggagccaccg
     5641 gctcgaaggc atggccagct tcaaaacctg cccctacacc gaccccaagg acatggtcgt
     5701 cctttcgggg accagggtgc gcgagatgct cggccagggg atcgtcccgc ccgaggaatt
     5761 cacccgccgc gagatcgccg aaatcctgat ccgccattac cgcgaggaag ctcgcctcga
     5821 ggccgaggaa cgccgcaagg ccaccggcga aaaggccggc ctcacccccg aagcctccgt
     5881 ctcggcccag cccgatgcct acgcctccaa gccccgctac gagcagggct tcacgatctg
     5941 gttcaccggc ctctccggct cgggaaagtc caccatcagc caggccctcg cgccgatcct
     6001 gcgcgcccgc ggacgcaccg tcgagatcct cgacggcgac gaagtccgcg aaaacctctc
     6061 caagggcctc ggcttcagca aggaagaccg cgacaccaac atcaagcgca tcggctacgt
     6121 ctgcaaactc ctctcgcgcc acggcgccgt cgccatcagc gcggctatca gcccctaccg
     6181 cgagatccgc gactataacc gctcccagat cggcaacttc atcgaaatct actgcgaggc
     6241 cccgctcgag gaactcgaaa agcgcgaccc caagggcctc tacaaaaaag cccgcgccgg
     6301 cgagatcaag ggcttcaccg gcatcgacga tccctatgag cccccgctca accccgagat
     6361 cgtcgtccac accgccaccg aatcggtgca ggagagcctg gacaagatca tccagtacct
     6421 cgaagcgaac gagtggctgc gctgaccggc gaggccggct ttcattcgcc cagtagtcat
     6481 ggaacaggac ttcctctgtc gcttgtgtcc cttatgtcgc ttacgtcctt ttcctccctt
     6541 attcttctag cgggagaatg actctccacc atggcggcgg cttccaccca gcaccacgaa
     6601 ttccctgacc tgcgcggacg ccacccacgc gaaatcctcg aatgggcctg ggccacctac
     6661 ggcgaccgcc tgatgacgac caccgccttc ggctacagcg gcatggccct gttccacatg
     6721 atggcgcagg tcgcgccgcg cgtgccgatc tacttcatcg ataccggcta tcacttcgcc
     6781 gaaacgctcc agtttcgcga ctactgccgc gacaaactcg gcctgaacat catcgatctg
     6841 cgccccgaga agacccgcga gcagtttgaa gccgagcatg gcggaccgat ctacaagacc
     6901 gaccccgacc gctgctgcgc gatcaacaag gtcgccccgc tgcagaagat gctccgcgag
     6961 tcgaactacg cggcc
//
LOCUS       DAOPCO010000210         6965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9699_length_6965_cov_5.323010, whole genome shotgun sequence.
ACCESSION   DAOPCO010000210 DAOPCO010000000
VERSION     DAOPCO010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9699_length_6965_cov_5.323010"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..595)
                     /locus_tag="PLS90_12935"
     CDS             complement(155..595)
                     /locus_tag="PLS90_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HPK03350.1"
                     /translation="MDAVLRGLSVYVFLLIVFRISGKRSLGQVTNFDFVLLLIIAETT
                     QQALLGDDFSITNSFILIVTLMLVDIALSLLKQRLPRLDRLLEGTPLVVVEDGRPIQA
                     YLRRARIDEEDILTAARGTQGLERMEQIKYAVLERNGDITIIPR"
     gene            complement(632..1147)
                     /locus_tag="PLS90_12940"
     CDS             complement(632..1147)
                     /locus_tag="PLS90_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03351.1"
                     /translation="MSDRGRVGDLEIDQDLVQQRRDWRVQRIGWAVMGLILLAACLGL
                     FGHGPLSRATAGDGGGPLQAEFERFCRLQTATTLRLRVQPAAVRDGRARLWLDREYLN
                     AVSLGAVVPEPERVEVGSDRIIYEFAVDESGEGPAVVSFELEPQRPGLLRGAAGLEDG
                     PSIPLRMWVHP"
     gene            1581..2177
                     /locus_tag="PLS90_12945"
     CDS             1581..2177
                     /locus_tag="PLS90_12945"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPK03352.1"
                     /translation="MLNHQVLVLNRYWVAVHVCTVRRALTLVFQELARIVSEDFQTYD
                     FESWRELSQFGRDAHPVIHTPNFSLLVPHVIVLSRYHRVPPRTVKFNRRNIFLRDQYC
                     CQYCGRRPHESELSIDHVVPRSRGGTSRWENVVLACVACNTKKGNRLLAECGMQLIRP
                     PRKPAWLSTLQQVHLTDDNRSLWEKFVDIAYWETNLRE"
     gene            2174..3862
                     /locus_tag="PLS90_12950"
     CDS             2174..3862
                     /locus_tag="PLS90_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03353.1"
                     /translation="MKRRRKARWIGAGALIAGVAAAALYWWLTPRVVTYPVEAFLPDR
                     LSAAFRISRFEPAWQRHWLRREGPGPEQAVQRVMQALGEWDDWLARYGEQGARLRLRA
                     YHGALFEALGEEAWLMFGEWGGDASGAGDVGFMAFFRGDTPLKARLGPLIELFLRDYR
                     FQTSRHHGVTLYEYRDERQGRSVTFCQVGGWICASMRRYGPGPLPTIIDRLKTFPRRA
                     IVASAPAWPESTRLRAGQPAFLPFADGAASQSALTLSFAPVAFWGHLRQFALQRDRGM
                     SDESEQRLQAWQLRLDGVDLISMRQWGPSLFNLEMKVAGPRPARLARQLDRDAEAAQP
                     AAPAPAPTDALPPELFQVDMAAPFARAILPLAGFSWEDVLEDLGDDLVWLAALRLRLL
                     EVLAGDDDAQDRGRVGVAAFESTTPMIPALLFWHDQPPLTASALAPAEHWARAALTGA
                     PAGDDVYLWLHPLELRPQAAAAPDQSAALAWHELTARSWQRPPRPPQGYVALSFGHVQ
                     QALQRVPAIFLDEKDREALERYQRLAEAFWLAFGGAVVRVDAEHGDWFLRLETM"
     gene            complement(3924..4844)
                     /locus_tag="PLS90_12955"
     CDS             complement(3924..4844)
                     /locus_tag="PLS90_12955"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPK03354.1"
                     /translation="MTLSPESVWPTYTRAAARVCLEAWAAIQPFYFGQACDVVDKPDG
                     PCTEADRLADRLIVEQLSRKFPRSEYGYLTEESEDDRERLERDRVWIIDPIDGTKDFI
                     ARRDNFCIQVGLAERLADGQWHPVAAAVYQPVAGQLWTATRGGGAFVQPVPFDPSHWA
                     NGRIPAPVGEPAPIHVTPIDRLDQARSVVSNANRSSRLMALIHSLELADYWHTGSLGV
                     KVCTIANGDAELYVNLGVGKTKEWDTAAPALILTEAGGRLTDFDGNTWTYNQEDVVHR
                     HGLLATNGAIHELTRARVKAFLEEHPTPYW"
     gene            5182..6258
                     /gene="prfA"
                     /locus_tag="PLS90_12960"
     CDS             5182..6258
                     /gene="prfA"
                     /locus_tag="PLS90_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPK03355.1"
                     /translation="MNRPDQRLETIRQQLAEIEALIAEPGAAHDPRRYAELTRRHREL
                     AEIRDAWAERDRLAGQLAQAEELAQEDDAEIAAMAREEIDATRAALADAEQTLRLLLL
                     PRDPNDARNTIIEIRAGTGGEEAALFAGDLFRMYARYAEIRGWRIELLSSHESDLGGF
                     KEVIFLVTGEDVFRRLKFEGGGHRVQRVPATEAQGRIHTSAVTVAVMPEAEDVEVELK
                     PEELRIDVFRSSGPGGQSVNTTDSAVRVTHLPTGLVVSCQDEKSQIKNKAKALKVLRA
                     RLLDLEQSRRHAAQAAQRKAQVGSGDRSERIRTYNFPQNRLTDHRIGLTLYSLEQIIE
                     GDLDPVIDPLIEHHQAELLRGAGM"
BASE COUNT         1115 a         2676 c         1999 g         1175 t
ORIGIN      
        1 gcccccgcga aataaccccc ccataacccg tcccgcgtcc tcgtccccga ccccaacgcc
       61 ccatcccaca attcggctct cggctctcgt ccctcgtcct cggctctcga atcaggccat
      121 ctccccgccc gccttctccc cccgcgcccc ccgctcaccg cgggatgatc gtgatatcgc
      181 cattgcgctc cagcacggca tacttgatct gttccatccg ctccagtccc tgcgtccccc
      241 gcgccgccgt caggatgtct tcctcgtcga tccgcgcccg cctcagatac gcctgaatcg
      301 gccggccgtc ctcgacgacc accagcggcg tcccctccag cagccggtcc agccgcggca
      361 gccgctgctt caacagcgac agcgcgatat ccacgagcat cagcgtcaca atcaggatga
      421 aggaattggt gatcgagaag tcatcgccca gcaacgcctg ctgcgtcgtt tccgcgataa
      481 tcagcagcag gacgaaatcg aagttcgtca cctgacccag cgacctcttg ccgctgatgc
      541 gaaacacaat cagcaggaac acatacaccg acagtccgcg caagacggcg tccatggcag
      601 gctccattct ctccttggcg gccgggcggg ttcagggatg gacccacatc cggagcggta
      661 tcgacggtcc atcctccagc ccggcggcgc ccctcagcaa cccgggccgc tgcggctcca
      721 gctcgaagct cacgaccgcc gggccctccc cgctctcatc gacggcgaac tcatagatga
      781 tccggtcgct ccccacctcg acgcgctccg gctcgggcac gaccgctccc aagctcacgg
      841 cgttcaggta ctcgcgatcg agccacagcc gggcgcgccc gtcgcgcacc gccgccggtt
      901 gaacgcgcaa ccgcagcgtc gtcgctgtct gcaaccggca gaatcgctca aattcggcct
      961 ggagcggacc ccctccgtcg ccggccgtcg cccggctgag cggaccatgc ccaaacaaac
     1021 ccaggcacgc cgccagcagg atgagaccca tgaccgccca gccaatccgc tgcacccgcc
     1081 agtcgcgccg ctgctgcacc agatcctggt caatctccag gtcgccgacg cggccccggt
     1141 ccgacatgct atctatcccc tgacgaacca tttgggcgga aaaccgcctg attccgtgga
     1201 aagcaattcg tgagccaaaa cagatgagcc agagcgttgc ctcacaatcc gtcgacaccg
     1261 ctttggatcc aatgaccggg gctgatgctc accggtccaa agcggcgtcg ccaagcgcgc
     1321 tgcgcgcttt ctgccgccgc acgccacgag ggcatcctgc tcgaaccggt cttccagcgg
     1381 ggggctgaac gagagaccaa ctctcgaacc gtgtcaagct gtgcccccgc caatcgattg
     1441 agactggacg agttgcgccg gctgcgttat aaattctaaa acctgtaccc gaccgcggcc
     1501 ggcgggggcg cacggcactt cggcagcgtc agtcagttgg atcgagtcgg catgcaggcc
     1561 gcttgagaac ggattgtcgt atgctcaacc atcaggtcct ggttctcaat cgctactggg
     1621 tcgcggtcca cgtctgcacc gtccgccggg cgctcacgct cgtcttccag gagctcgccc
     1681 gcatcgtctc ggaggacttc cagacctacg acttcgaatc ctggcgcgag ctctcccaat
     1741 tcggccgcga cgcccacccg gtcatccaca cccccaattt cagtctgctc gtcccgcatg
     1801 tgatcgtcct gtcgcgctac caccgcgtgc cgccgcgcac cgtcaagttc aaccggcgca
     1861 acatcttcct ccgcgaccag tattgctgcc agtattgcgg gcgccggccg catgagagcg
     1921 agctgtcgat cgaccacgtc gtcccccgct cgcgcggcgg cacctcccgc tgggaaaacg
     1981 tcgtcctggc ctgcgtcgcc tgcaacacga agaaaggcaa ccggctcctc gccgaatgcg
     2041 ggatgcagtt gatccgaccg ccgcgcaaac cggcgtggct ctccaccctg cagcaggtcc
     2101 acttgaccga cgacaatcgc tcgctgtggg aaaagttcgt cgatatcgcc tactgggaaa
     2161 ccaacctccg cgaatgaaac gccgtcgcaa ggcgcgctgg atcggcgccg gcgcgctgat
     2221 cgcaggggtg gccgccgccg ccctctactg gtggctcacg ccgcgcgtcg tcacctatcc
     2281 ggtcgaagcc ttcctccccg accgcctctc ggccgcgttc cgcatctccc gcttcgaacc
     2341 ggcctggcag cgccactggc tgcggcgcga gggacccggg ccggagcagg ccgtccagcg
     2401 cgtcatgcaa gccctcggcg aatgggacga ctggctcgcc cgctacggcg aacagggcgc
     2461 gcgtctccgc ctgcgcgcgt atcacggcgc gctgttcgag gccctcggcg aggaagcctg
     2521 gctgatgttc ggcgaatggg gcggcgacgc ctccggcgcg ggcgatgtcg gcttcatggc
     2581 cttcttccgc ggcgacaccc cgctcaaggc ccgcctcggc ccgctcatcg agctttttct
     2641 gcgcgattac cgcttccaga ccagccgcca ccacggcgtc acgctgtatg aataccgcga
     2701 cgagcgccag ggccgcagcg tcaccttctg ccaggtcggc ggctggatct gcgcctcgat
     2761 gcggcgctac ggtcccggcc cgctgccgac gatcatcgac cggctcaaga ccttcccccg
     2821 ccgcgccatc gtggccagcg cccccgcctg gcccgagtcg acccggttgc gcgccgggca
     2881 accggccttc ctccccttcg ccgacggcgc cgcttcgcag tcggcgctga ccctcagttt
     2941 cgcccccgtc gccttctggg gtcacctgcg ccagttcgcg ctgcagcgcg accgcggcat
     3001 gtccgacgag tccgaacagc gcctccaggc ctggcagctc cggctggacg gcgtcgatct
     3061 gatttccatg cggcagtggg gcccctcgct gttcaatctc gagatgaagg tcgcgggccc
     3121 ccgcccggcc cggctggccc gccagttgga ccgcgacgcc gaagccgccc agcccgccgc
     3181 gccggcgccc gcgccgaccg acgcgctgcc ccccgagctg ttccaggtcg acatggcggc
     3241 cccctttgcc cgggcgatcc tcccgctggc cggcttctcc tgggaggatg tccttgagga
     3301 tctgggcgac gacctcgtct ggctggcggc gttgcggctc cggctcctgg aggtcctggc
     3361 cggcgacgac gacgcccagg atcgcggccg cgtcggcgtc gccgccttcg aatcgacgac
     3421 gccgatgatt cccgcgctcc tcttctggca cgaccagccc ccgctgacgg ccagcgccct
     3481 cgccccggcc gagcattggg cgcgcgccgc gctgaccggc gcaccggccg gcgacgatgt
     3541 ctatctctgg ctccacccgc tggaactgcg cccccaggcg gcggccgcgc ccgaccaatc
     3601 ggcggcgctg gcctggcacg agctgaccgc ccggagctgg caacgcccgc cgcggcctcc
     3661 ccagggctac gtcgcgctca gcttcggcca tgtccagcag gcgctccagc gcgtgccggc
     3721 gattttcctc gacgagaagg atcgtgaagc gctcgagcgc taccaacggc tggccgaggc
     3781 gttctggctc gcgttcggcg gcgccgtcgt ccgcgtcgac gccgagcacg gcgactggtt
     3841 cctccgcctc gaaacgatgt aacccacgcc cctcagcggt aatcggtatc ggtaacgcat
     3901 tcgcgtcgat cccgccctcg ccctcaccaa tacggcgtcg ggtgttcctc cagaaacgcc
     3961 ttgacccgcg cgcgcgtcag ttcgtgaatc gcgccgttag tcgccagcag cccgtggcga
     4021 tgcaccacgt cctcctgatt gtaggtccag gtgttcccgt cgaagtcggt cagccgcccc
     4081 cccgcctcgg tcaggatcag cgccggcgcg gccgtatccc actccttggt tttcccgacg
     4141 ccgaggttga catacaactc cgcgtcgcca ttcgcaatcg tgcagacctt cacccccagc
     4201 gagccggtgt gccagtagtc ggccagctcc aggctgtgga tcagcgccat cagccggctg
     4261 gagcggttgg cgttgctgac gaccgagcgg gcctggtcca gccggtcgat tggggtgacg
     4321 tggatcggtg ccggctcgcc gaccggagcg gggatccgac cgttcgccca gtgcgacgga
     4381 tcgaacggga cgggttgcac gaacgcgccg cccccgcgcg tcgccgtcca cagctggccg
     4441 gcgaccggtt gatacaccgc cgcggccacc ggatgccact ggccgtcggc caggcgctcg
     4501 gccagcccca cctgaatgca gaagttgtcg cgccgcgcga tgaagtcctt cgtgccgtcg
     4561 atcgggtcga tgatccacac ccggtcgcgc tcgagccgct cccggtcgtc ctcgctttcc
     4621 tccgtcaggt agccatactc cgatcgcggg aacttccggc tcagctgttc gacaatcagt
     4681 cgatccgcga gccggtcggc ctcggtgcag ggtccatcgg gcttgtccac gacgtcgcac
     4741 gcctggccaa agtagaaggg ctgaatcgcg gcccatgcct caaggcagac ccgcgccgcc
     4801 gcccgcgtat aggtgggcca gaccgattca ggggataacg tcatggatga aattccttac
     4861 tcaccggttc gtccgtcacg ctgaaatcgt ccctcgtcgc tcgtcttcgt cctcgttcct
     4921 cacccccaaa ggccctcccc agcattcgtc tcccagcttc cgtccctcgt cctcggctca
     4981 cgaaaaccgg gcctcacgca ttctaccggc gccccggcgc atcttcaatc cgcccgactg
     5041 tccccactgt ccggcgggtt cggcccgcgt accgcgtcgc tcgccatcgc gccgaactcc
     5101 cattgccctt tacccccgtt tccgttgaaa tggaacggcg tccggcgcgc ccgcgcgttc
     5161 atcccaccgg cccctcgacc catgaaccgt cccgaccagc gccttgaaac gatccgccag
     5221 cagttggccg aaattgaagc cctgatcgcc gagcccggcg cggcgcacga tccgcggcgc
     5281 tacgccgaac tgacccgccg tcaccgcgaa ctcgccgaaa tccgcgacgc ctgggccgag
     5341 cgcgaccgcc tcgccggcca gctcgcacag gccgaggaac tggcacagga ggacgacgcc
     5401 gagatcgccg cgatggcccg cgaggagatc gacgccacac gcgccgcgct ggccgacgcc
     5461 gagcagaccc tgcgcctgct gctgctgccg cgcgacccca acgacgcccg caacacgatc
     5521 atcgagatcc gcgccggcac cggcggcgag gaagccgccc tgttcgccgg cgacctgttc
     5581 cgcatgtacg cgcgctacgc cgaaatccgc ggctggcgca tcgagctgct ctccagccac
     5641 gagtcggacc tgggcgggtt caaggaggtg atcttcctcg tcaccggcga ggacgtcttc
     5701 cgacggctca agttcgaggg gggcggccat cgcgtccagc gcgtgccggc caccgaggcg
     5761 cagggccgga tccacacctc cgccgtcacc gtcgccgtca tgcccgaggc cgaggatgtc
     5821 gaggtcgagc tcaagcccga agagctgcgg atcgacgtct tccgctccag cgggccgggc
     5881 gggcagtcgg tcaacacgac cgattcggcc gtccgcgtca cgcatctgcc gaccggcctg
     5941 gtcgtctcgt gccaggacga gaagagtcag atcaagaaca aggccaaggc gctcaaggtg
     6001 ctgcgcgccc ggctgctcga cctcgagcag agccgccggc acgccgcgca ggccgcccag
     6061 cgcaaggcgc aggtcggcag cggcgaccgc tccgaacgga tccgcaccta caatttcccc
     6121 cagaaccggc tgaccgacca tcgcatcggc ctgacgctct acagcctgga gcagatcatc
     6181 gaaggcgact tggaccccgt gatcgacccg ctgatcgaac atcaccaggc cgaactgctc
     6241 cgcggcgccg ggatgtaagt cgcttctcac ccttcgtcga cttctccctt tgcgtcgctt
     6301 ggttgctgcc aacttgcgcc gccgccgcgc cgggctttcc cgggccgcat ccctcttctc
     6361 actcaagaat ccgtctctca tcctcattta agtatcgtcc ctcgtcctca tcaagaatcg
     6421 tcactcgcct tgcgtcctcg tgattgcccc caacggcccg ccccggtttt tctccatcgt
     6481 tgtgagcccc gattcatcag aaactcgtac gaacattagt gtttcaacgg gacactaagg
     6541 actgcatgca caacattttt atcctttcag agaaacggcg acggcgtcgt aaaaagctcg
     6601 cgcgcaaatt acgctccgct aagttcctct aagggtccct aagggaattc ctttttccct
     6661 ctccttcgcc gcataataat ccccaacacc gagcgctccc aaacgcccgc tggtctccca
     6721 tcttgtaaca ctccggagca gttagccccc gttacatcat ttatattagc atttcgaatt
     6781 tgtttcgtac ttcgaaattc gtatttcgaa ttctcttctt cccatgtcca accccaacct
     6841 cgccaccatc tcccactgca tcgacgcctt cgaagccgtc ctcctgcgct cgatcgtccg
     6901 cctcgccggc gacctcgacc tcaccgccgc cgacctcccc ggcgagctct ccccgatcca
     6961 tgccc
//
LOCUS       DAOPCO010000211         6944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9729_length_6944_cov_5.385107, whole genome shotgun sequence.
ACCESSION   DAOPCO010000211 DAOPCO010000000
VERSION     DAOPCO010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9729_length_6944_cov_5.385107"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..993)
                     /locus_tag="PLS90_12965"
     CDS             complement(127..993)
                     /locus_tag="PLS90_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03356.1"
                     /translation="MLPRLLPIELICACLVPTALATPQQVAAQAEMATLAEGLTQCAV
                     DTSWYVSLETLNDVAAANLEPHYDSLNDAGGVWVLRPGRGLFHPARRDLASGVLRWRG
                     PYVNFQSGRTQTGPEPYDLGSPLDPWGRPYLLFSPLGLVRGDGGTVTQEYYGDAFDRY
                     TIVTLGFDGVMSEDDQFHAFGAGITDFVISSARAVDELKAEGDALPAGGVIRIRGYNL
                     GISPEDGQVVLGDRVLTDVSSWTPVAVEVAIPADVRGPAPLFLRRGALETNRIEVQIA
                     GPNAARGWTCYP"
     gene            1223..2389
                     /gene="ispG"
                     /locus_tag="PLS90_12970"
                     /gene_synonym="gcpE"
     CDS             1223..2389
                     /gene="ispG"
                     /locus_tag="PLS90_12970"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPK03357.1"
                     /translation="MNPQPLPAPRRRAREVKLGPLILGGDQPIRVQSMTATDTADPDA
                     TVEQINALAEAGCEIVRLTVDTPKAARALPDIRRRTAVPLVADIHYNHRLALEAAPYV
                     DKIRINPGNIGALENVKAVVARANEFDLPIRIGVNQGSLEREIGLKYGAHLLDNVLLP
                     PEEGYPAEALVESALRNVEILEGFGFERIIISVKSSNVPLMVRAYRLLAEACDYPLHL
                     GVTEAGTKDNSNIKSSIGIGALLLDGIGDTLRVSIAARATEEKIEEVRTGYKILQALG
                     LRRFGVEVVSCPTCGREDQGFDTTRIALQLEELNSHLTRPIKLAVMGCYVNGPGEAAD
                     ADLGVVASGKTAKIYRSGRLIQTQVPFEEVVDRMSELIRQIDREQREEITPVPT"
     gene            complement(2545..4359)
                     /locus_tag="PLS90_12975"
     CDS             complement(2545..4359)
                     /locus_tag="PLS90_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03358.1"
                     /translation="MKSIFALFVSVALFLGATAPRALDVVLLSDTFDGGTVGAYLDGQ
                     PLNNGLGGSVSATWEDPGKYATYRQIGTNMVADVSRGPWDVYNFLTSYEVDPTKTYAV
                     EFVQYLPEGTLDPDYWNPHGGVVVMARDQHMAGGGLVIRLQGLDASGWDPAYFSLVYY
                     NGERFFPNPPIFTGNFELEDFNSDGALPVRLEFTGAGTPESPLNGRLFLNGNFKGSFS
                     IDDMVNAGRKVGFVTQTGQRPPGADHGWTDNFVLSEVVLPPTVEILAGTFPYFLPHNQ
                     VPLDAIVLKAGLTEDPSLEVTWSQVSGPATVTFGDIHAIQTDVTLPAVGYYTLKLEAR
                     DGTLIGSDTVELVLAEAPGETVILSDNFDGGTAGNNIGGRTLNNALGGSLSATWADTL
                     AMAKYGLDGSDLVCHLGPVTTWDEVKFTCTFEADPNKLYALETRIKPATGVDGIYNRA
                     CGPVLYSQNDQRFQRSGLNFRFSGPADNEEAPNSVNLVDINGTRVTGWTWSSLVVGAD
                     DPRIDPLNDYDDNGYLPIRIEFTGTGTPDNPAEGRVYINGNWKCDFVADDLGNPGRRF
                     GYQTTQLSSSNPLVIGDVVVSELNVDLPNAARHWSVLQ"
     gene            complement(4444..6204)
                     /locus_tag="PLS90_12980"
     CDS             complement(4444..6204)
                     /locus_tag="PLS90_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03359.1"
                     /translation="MSDTFENGTIGDSVHEQPLDNALGGSASATWLDPLGIATYQVDG
                     ASQVMSVARPAGGWSKFKTLCSYPTDPNKSYVLEFRQKRPTGVLDGDNWTAHGGATVL
                     STDNIEMDGGMGGSGIILRLQSAAVPGANDPDWYSVFANRGGNMFSHHMMSGPITAED
                     VAGDGYIPVRILFSGAGTAESPATFKLYLNGNEKGSFTVDNLANPGNLVGVINQTSTL
                     PDYDPGMVDDFKLAEITGVDPTVTIAQKDRYPVYLPTDTVLVSGSGVKDGFVPMTSGL
                     TWSTESGPAEAIFADPNAATTEVTFPQPGTYVLKLTLDTGSATATDTITFVVRAGILT
                     ATPILSDDFEGADDGDPLNGRVLNNALGGAETATWSDDLGLAWLADSSGDVICKLGPN
                     AGWPNTKITCDYEIDPTRSYRLEALLKLPTGIDGTAPAWNRPAGPGLLSEADQGIDHC
                     GVIFRFTSDSPNEGLPNTVNVIDVNGRLTGGWSWNSLRVGGWGQIDPATDLDANGWLP
                     VCIEFTGDGTPANPLRGRILLNGNYRGEFTTSDLGAHGYRISFSTTQFSQDDPLQIAQ
                     FVLSELEWEVPAELSEFMVE"
     gene            complement(6466..6846)
                     /locus_tag="PLS90_12985"
     CDS             complement(6466..6846)
                     /locus_tag="PLS90_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03360.1"
                     /translation="MPVPNGYAFNFLTTPIAFLSSIPTDKFFDVKMWPVEPPRYWYQG
                     AGFQNHPVVKARAIYGFRGKWIASSHGPDLFCDFGEAAILAGELTHMTVATNGTIVIR
                     RWPDMIYDPTNGTVSSGDLVRWGP"
BASE COUNT         1199 a         2267 c         2284 g         1194 t
ORIGIN      
        1 cggactgggc caccagcggc gcggtcgagg tgcgggcgga cgttgcgccg ccatccggcg
       61 cgagttcgta aggctcccga accgtccggc ggccgcgatc gccgaggcaa gccctgtcgc
      121 gatcgctcat gggtagcacg tccagccgcg agccgcgttg gggccggcga tctggacttc
      181 gatgcggttg gtctccagcg caccccggcg cagaaagagc ggcgctggcc cgcgcacgtc
      241 cgcgggaatc gccacctcga ccgcaaccgg cgtccagctg cttacatcgg tcagcacccg
      301 atcgccgagg accacctggc cgtcctccgg cgagattccc aggttgtagc cgcggatgcg
      361 gatcacgccg ccggcgggca gcgcgtcgcc ttcggccttc agctcgtcca ccgcccgcgc
      421 gctggagatg acaaaatcgg tgatccccgc gccgaacgca tggaactggt cgtcctcgct
      481 catcacgccg tcgaagccga gcgtcacgat cgtgtaacgg tcaaacgcgt cgccgtaata
      541 ctcctgcgtc accgtgcccc cgtcgccccg cacgagaccc agcggcgaga agagcagata
      601 cggccggccc cacggatcga gcggcgatcc caagtcatag ggttccggtc cggtctgggt
      661 gcggccggat tgaaaattga cataaggtcc gcgccagcgc aggacgccgc tggcgaggtc
      721 ccgccgagcg ggatggaaca gcccgcgccc cggccgcaag acccacacgc cgcccgcgtc
      781 attcagcgaa tcatagtgtg gctcgaggtt cgccgccgcg acgtcattca acgtttccag
      841 cgaaacatac cacgacgtgt ccacggcgca ctgggtcaag ccttcggcca gcgtcgccat
      901 ctcggcctgg gcggcgacct gctgcggcgt cgcaagcgcc gtcggaacca ggcaggcgca
      961 gatcaactcg atggggagca gccggggcag catcaaatca actccaaggt cattctagcg
     1021 catccggcgt cggttccgcc caccggctat catggaaagc gaacaaatcc gacacgcgcc
     1081 tgctgttttt cccgatctcg ttttcctcgt gattccggcc cgaccatggc tagaatggag
     1141 gggcggctcc ggccccgagc atgatactca ggaaggccga gccagccgcg ggcgtcgtcc
     1201 cacccgcaag gaaatgaaaa tcatgaatcc ccagcccctg ccggcgccgc gccgccgggc
     1261 ccgcgaagtc aagctcggcc ccctgatcct tgggggcgac cagccgattc gcgtgcagtc
     1321 gatgactgcc accgacacgg ccgatccgga tgcgaccgtc gaacagatca atgctctggc
     1381 cgaggccggt tgcgaaatcg tccgcctgac cgtcgacacg ccgaaggccg cccgcgccct
     1441 gcccgacatc cgccggcgga cggccgtccc cctggtcgcc gacatccatt acaaccaccg
     1501 cctcgcgctc gaagccgccc cttacgtcga caagatccgg atcaatcccg gcaacatcgg
     1561 ggcgctggag aacgtgaagg cggtcgtcgc gcgcgccaac gagttcgatc tgccgatccg
     1621 catcggcgtc aaccagggct cgctcgagcg cgagatcggg ctgaaatacg gcgcgcatct
     1681 gctggataac gtcctgctgc cgccggagga gggctacccg gccgaggcgc tggtcgaatc
     1741 ggcgctgcgc aatgtcgaga tccttgaagg cttcggcttc gagcgcatca ttatttcggt
     1801 caagtcgtcc aatgtgccgc tgatggtgcg ggcctaccgg ctgctggccg aggcatgcga
     1861 ctatccgctc cacctgggcg tgaccgaggc ggggacgaag gacaacagca acatcaaatc
     1921 atcgatcggg atcggcgccc tgctgctcga tgggatcggc gacacgctgc gggtttcgat
     1981 cgccgcccgc gccaccgagg agaagattga ggaagtacgc acgggctaca agatcctgca
     2041 ggcgctcggc ctgcggcggt tcggcgtcga ggtcgtcagt tgcccgacct gcgggcgcga
     2101 ggaccagggg ttcgacacga cccggatcgc actgcagctc gaggagctca attcccatct
     2161 gacgcggccg atcaaactgg ccgtgatggg ttgctacgtc aacgggcccg gcgaggcggc
     2221 cgatgccgac ctgggcgtcg tcgccagcgg caagacggcc aagatctatc gctccggccg
     2281 gctgatccag acgcaggtgc cgttcgagga agttgtggat cggatgagcg agctgatccg
     2341 ccagatcgac cgcgagcaac gcgaggagat cacgccggtg ccgacgtgat ccgccgttgc
     2401 gcgcagagca aagagaaagc cgccggatcg atccgatcag accgatccgg cggatttctt
     2461 tagccgcaat gaaaaagccg gctcgcgcgg gttgagcccc cgcgagccgg ccaggaatca
     2521 ctccattgcc ggtcgtccgc cgacctactg cagcacgctc cagtggcgcg cggcgttggg
     2581 cagatcgacg ttcaactcac tgacgacgac gtcgccgatg accagcgggt tgctcgagct
     2641 gagctgcgtc gtctggtaac cgaagcggcg gcccgggttg cccagatcgt ccgcgacgaa
     2701 gtcgcatttc cagttgccgt tgatgtagac gcgaccctcg gccgggttat ccggcgtgcc
     2761 ggtgccggtg aactcgatcc ggatcggcag atagccgttg tcgtcgtagt cattcagcgg
     2821 gtcgatgcgc ggatcgtcgg cgcccacgac caggctgctc cacgtccacc cggtcacgcg
     2881 cgtgccgttg atgtccacca ggttgaccga attgggcgcc tcttcattgt cggcggggcc
     2941 ggagaagcgg aagttcaggc cgctccgctg gaaccgctgg tcattctgcg agtagaggac
     3001 cggaccgcag gcgcggttgt agatcccgtc gacccccgtg gccggcttga tccgggtttc
     3061 cagcgcatac agcttgttcg ggtcggcctc gaaggtgcag gtgaacttga cctcatccca
     3121 cgtggtgacg gggcccaggt ggcagaccag atcgctgccg tccaggccgt acttcgccat
     3181 cgcgagcgtg tcggcccagg tggcggagag cgagccgccc agcgcgttgt tcagcgtgcg
     3241 gccgccgata ttgttgccgg ccgtgccgcc gtcgaagttg tcgctgagga tcacggtttc
     3301 accgggcgct tcagccagca ccagctccac cgtatcggag ccgattagcg tgccgtccct
     3361 ggcctccagt ttgagcgtgt aataccccac ggcgggcagg gtcacgtcgg tctggatcgc
     3421 atggatgtcg ccgaaggtga ccgtcgcggg accgctgacc tggctccagg tgacttcgag
     3481 cgacgggtcc tcggtcagcc ccgccttgag cacgatcgcg tcgagcggca cctggttatg
     3541 gggcaggaag taagggaagg tccccgccag gatctcgacc gtgggcggca gcaccacttc
     3601 cgacaggacg aagttgtccg tccagccgtg gtcggccccg ggcgggcgct ggccggtctg
     3661 cgtgacgaac ccgaccttgc ggccggcgtt gaccatgtca tcgatcgaga aggaaccctt
     3721 aaagttgccg ttgaggaaca gccggccgtt gagcggcgac tccggcgtgc cggcgccggt
     3781 gaactccagc cgcaccggga gcgcgccgtc cgagttgaag tcctccagct cgaagttgcc
     3841 ggtgaagatc ggcggattcg ggaaaaagcg ctcgccgttg taataaacga ggctgaagta
     3901 ggccggatcc catcccgagg catccagacc ctgcagccgg atcaccaggc cgccgccggc
     3961 catgtgctgg tcccgggcca tgacgaccac gccgccgtga gggttccagt agtcgggatc
     4021 gagcgtccct tcgggcagat actgcacgaa ctcgacggcg taggtcttcg tcggatcgac
     4081 ttcatacgac gtcaggaagt tgtacacatc ccacgggccg cggctgacgt cggccaccat
     4141 gttggtgccg atctggcggt aagtggcgta cttcccgggg tcctcccagg tggcgctcac
     4201 gctgcctccg aggccgttat tgaggggttg accgtcgagg taggcaccga ccgtgccgcc
     4261 gtcgaacgta tcactcagaa gcacaacatc cagggcgcgc ggcgccgtgg cgcccaggaa
     4321 cagcgccacc gagacgaaca gagcgaagat tgatttcatg atgctctctc tcctccttgc
     4381 agattcaagg gaaaggccgg gagggaagcc ggccgtccgg tccctcccgg catcgtggtc
     4441 aggctattcc accatgaact ccgagagttc ggccggaact tcccactcca gctcgctcag
     4501 cacgaactgc gcgatttgca gtggatcgtc ctggctgaac tgcgtcgttg agaagctgat
     4561 ccggtagccg tgggcgccca ggtccgaggt ggtgaactct ccccggtaat tgccgttcag
     4621 gagaatccgt ccacggagcg ggttggcggg cgttccatca ccggtgaact cgatgcacac
     4681 cggcagccag ccattcgcgt ccaggtcggt cgccggatcg atctggcccc agccgccgac
     4741 gcgcagcgag ttccagctcc acccgccggt cagccggccg ttgacgtcga tcacgttgac
     4801 cgtgttggga aggccctcat tgggcgaatc gctggtgaag cggaagatca cgccgcaatg
     4861 gtcgatcccc tggtccgcct ccgacaggag gcccggcccg gccggccggt tccacgccgg
     4921 cgccgttccg tcgatcccgg tcggcagctt cagcagcgct tcgagccggt agctgcgggt
     4981 cgggtcgatc tcgtagtcgc acgtgatctt cgtattcggc cagccagcgt tggggccgag
     5041 cttgcaaatc acgtcgccgc tgctgtcggc cagccaggcc agcccgagat cgtcgctcca
     5101 ggtggcggtc tccgcgccgc ccagggcgtt gttcagcacg cggccgttga gcgggtcgcc
     5161 gtcgtcggct ccctcaaagt cgtcgctcag gatcggcgtc gccgtcagga tgccggcccg
     5221 cacgacgaac gtgatcgtgt cggtggcggt ggccgatccg gtgtcgagcg tcagcttcag
     5281 cacgtaggtc cccggctgcg ggaacgtgac ttcggtggtc gccgcgttgg gatcagcgaa
     5341 gatggcctcg gccggtccgc tctcggtgct ccaggtcagt ccgctcgtca tcgggacgaa
     5401 gccgtccttg accccgctgc cgctgaccag cacggtgtcg gtcggcagat agaccgggta
     5461 gcggtctttc tgcgcaatcg tgaccgtggg gtcgacgccg gtgatctcgg cgagtttgaa
     5521 gtcatcgacc attcccgggt cgtagtccgg cagcgtgctc gtctggttga tcacgccgac
     5581 caggttgccg gggttggcca ggttgtcgac ggtgaaggaa cccttttcgt tgccgttcag
     5641 atacagcttg aacgtggcgg gcgactcggc ggtgccggcg ccggagaaca gaatccggac
     5701 cgggatatag ccgtcgccgg ccacatcctc cgcagtgatg ggccccgaca tcatgtggtg
     5761 gctgaacatg ttgccgccgc ggttggcgaa gaccgaatac caatcggggt cattggcccc
     5821 ggggacggcc gccgattgca gccgcaggat gatgccgctg ccgcccatgc cgccgtccat
     5881 ctcaatgttg tcggtcgaca ggaccgtcgc gccgccgtgc gccgtccagt tgtcgccgtc
     5941 gaggaccccc gtcggccgtt tctgccggaa ctccagcaca tacgacttgt tggggtcggt
     6001 cgggtatgaa cacagcgtct tgaacttgga ccagcctccg gccggacgcg ccacgctcat
     6061 cacctggctg gcgccgtcga cctgataggt cgcgatgcca agcggatcca gccaggtggc
     6121 tgaggcggaa ccgcccaagg cgttatccag cggctgttca tggacggaat cgccgatggt
     6181 gccgttctca aacgtatcgc tcacgacgat ggtctcggct gcatgaatcg atatgaccga
     6241 tacggagaga aaggcgagaa ccgtcaagag acgaatgatt ctcgaatgcc gcatgatagc
     6301 tctctcctcc atcataaata ggacacctga gattcgatgg gttgaatcag ggggactcaa
     6361 cctgtccgcg cgcgtgattg agtggtgccg ccctcctttc ggcgaaaagt ggtacagcct
     6421 gaaacccgcg ccgcccgccc cgccggcgcg tccgcgaacg tcacgtcacg gtccccagcg
     6481 gaccaagtcg ccgctgctga ccgtcccgtt ggtcgggtcg taaatcatgt cgggccagcg
     6541 ccggatcacg atcgtgccgt tggtggcgac ggtcatgtgc gtcagttcgc cggcgaggat
     6601 ggcagcctcg ccgaaatcgc agaacaggtc cggaccgtgg gagctggcga tccatttgcc
     6661 gcggaagccg tagatcgccc gcgccttgac gaccgggtga ttctggaacc cggctccctg
     6721 ataccagtag cgcggcggct caaccggcca catcttgacg tcgaagaact tgtcggtcgg
     6781 gatcgaggac aggaacgcga tcggcgtcgt caggaagttg aacgcgtagc cgttgggcac
     6841 gggcatgccg gccagtgtca gctcatcgcg atcgggggga tagtgggcgt agaactcgtt
     6901 tcataactgc aaatgcttga gactgcttga catattttac ttgt
//
LOCUS       DAOPCO010000212         6921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9762_length_6921_cov_5.058841, whole genome shotgun sequence.
ACCESSION   DAOPCO010000212 DAOPCO010000000
VERSION     DAOPCO010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9762_length_6921_cov_5.058841"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1612
                     /locus_tag="PLS90_12990"
     CDS             194..1612
                     /locus_tag="PLS90_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPK03361.1"
                     /translation="MKTITIHPVTRIEGHARITLHLDEQGRVADSHFHVTQVRGFEKF
                     TEGRPFYEMPSVMARICGICPVSHLLASSKACDAVMAVRVPPAGARLRSILHFGQFVQ
                     SHALSFFHLSSPDLLLGMDSDPARRNIFGVIEKHPELAREGIALRKFGQQVIEWLAGE
                     RVHPAWVVPGGVNAPLTADVRDRILREIPSARAITMKTLALLKRVVDQFTEEIEHFGN
                     HPSLYLGLVDPRGNLELYDGRLRIMDDMGKFMTDQVDPETYQDLIGEAVLPTSYLKAP
                     FYKPLGYPRGIYRVGPLARINLCDQCGTPAADAELAEFRQRFGRPARGAFHYHYARLI
                     EVIFCLEKMEELLDNPNVLDPHVRAEAGVNAEEGIGVAEAPRGTLIHHYRVDPHGTIR
                     WANLIIATGHNNLAINQAVLQVARHFVDGNKLREGMLNRVEAVVRCYDPCLSCSTHAI
                     GQMPLRIQLVGADGQVLDEQHR"
     gene            1636..2121
                     /locus_tag="PLS90_12995"
     CDS             1636..2121
                     /locus_tag="PLS90_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPK03362.1"
                     /translation="MPRSTLIIGYGNTLRGDDGAGPAVVDRLQRRGPRPGPRLRAAHQ
                     LTPELAEELSRHARVVFIDARAAEPPGAIVCEPLQPAADAGGAALTHQFSPAMLLLLA
                     RRLYGRQPAAWLIGINGADFDPGEGLSPAVARAVDLVAARWQALIAQTPKESTPCMKR
                     P"
     gene            2103..2369
                     /locus_tag="PLS90_13000"
     CDS             2103..2369
                     /locus_tag="PLS90_13000"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPK03363.1"
                     /translation="MHEASLMDSLMFAINQSARAHGAKRVKWVRVRIGAHCHLSAAHL
                     QHHYDEAAAGTVAEGAQLEIIQDDNETSPHADIVVLDSLEIETD"
     gene            2570..2947
                     /locus_tag="PLS90_13005"
     CDS             2570..2947
                     /locus_tag="PLS90_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03364.1"
                     /translation="MIEDFVRIHTIHFQTARRPSPGWLAPLVLVGVTLLVSGAVLVTL
                     ACSAVLFAARGLSRLIGRPFEKILDSAARGNGAPAGRETAHGRPASFSDPAGGVAPRR
                     AASPAPRTIDLAPDAAGVWREDE"
     gene            complement(3027..5105)
                     /gene="dnaX"
                     /locus_tag="PLS90_13010"
     CDS             complement(3027..5105)
                     /gene="dnaX"
                     /locus_tag="PLS90_13010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPK03365.1"
                     /translation="MALTESYQGLARKWRPQTFADLVGQEAVAKSLQNALRTGRVSHG
                     HLLAGPRGVGKTTSARLLAKAINCAQGPTPEPCGACRHCIDIAQGTDLDVIEIDAASN
                     TGVDNIRDLRERIVQAPFAARYKVYIIDEVHMLSTGAFNALLKTLEEPPPFVVFIFAT
                     TELEKVPETIRSRCTLHAFRRMNAEDIARRLDQVAEGEGVKLEPAGRRELHGLIARSV
                     EGGMRDALVIYDQLLAMTEGKPDAEAAARLLGLADQSALARATEWLAEGNAAGLLELI
                     EDLVIRGRSLERFIRSLVAYQRDILLLQSGVGDKLVMTTGEALGEARRLAEAISPATL
                     YNILNQMFELEERIKGSSQARFLIEFTFLRVAAIKPVVPIDEIVKRIGALPEEAFESA
                     GRATSAAAARSAAAGSDDPARREAHAATETGTAEPAASVAETSAAELRRPRAAVGGRG
                     VGAGRAPAFMDSAPPMEAAEVAAARSEEESPAAGAASVGEIVAPEALLPRLLAQCSEN
                     ARGLVRRYLVKAAGCELGAAALKIYWRAEDGFSRRILENPENIGVMEQALARLCGRPM
                     KITHLDAPAGLTAAPAPEGTAAGGAKAAAAQRTAPTLADSAPPVDDEMPMENDAVEEE
                     NSIGIAEAGTERAVTRASVARAPVDRRTAKERAQALMARDGEIARRVKLLREMFRGQI
                     IDETGQPISV"
     gene            complement(5252..6649)
                     /gene="hslU"
                     /locus_tag="PLS90_13015"
     CDS             complement(5252..6649)
                     /gene="hslU"
                     /locus_tag="PLS90_13015"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPK03366.1"
                     /translation="MTTQVELTPRQIVSELDKFIVGQAEAKRVVAIALRNRTRRRRLD
                     EALQEEVMPKNIIMIGPTGVGKTEIARRLARLVRAPFVKVEATKYTEVGYVGRDVESM
                     VRDLVETAVNMVREEQIEAARPRAEKRARERLIDLLHKGPQGGGRRRFLGGFGTRTEA
                     QVEGGEADEEPDGEELRRNLEKLLDEGRLEDREVEFEVTARPVVDGMFGMIGVDEQML
                     GGLREMMEKMMPQKRKLTRMKVREARKVLFDEEVERLLDSEKIKLIALERAEQNGIIF
                     VDEIDKIAGRHAGQGPDVSREGVQRDILPIVEGSTVNTKHGAIRTDHILFIAAGAFHA
                     TKPSDLIPELQGRFPLRVELEALQVEDLKRILVEPENSLIKQYVALLRTDGVELEFTD
                     DAIDELARSAATINAQSENIGARRLHGILERVLEDVSYDAPDEARGRVVVDAEYVKER
                     LSKLMENKDLSQFIL"
BASE COUNT         1014 a         2834 c         1892 g         1181 t
ORIGIN      
        1 gccgccacgc cgcccgacga ggagccgccg tgcgaggagg ggcagccgtt cgacgagagc
       61 gtgctgcagc acatgcccat cgaggaccag atcaagttca gcgggctgct cgccggccgc
      121 acccccaacc tcgaaacgcg cgcgcggttc ggctgagccc acgccctcat tcacgacaag
      181 gatgaccacg ccgatgaaga cgatcacgat ccatccggtc acgcgcatcg aagggcacgc
      241 ccgcatcacg ctccacctgg acgagcaggg gcgcgtcgcc gacagccact tccacgtcac
      301 ccaggtgcgc ggcttcgaga aattcaccga gggccgtccg ttctacgaaa tgccctcggt
      361 catggcgcgc atctgcggca tctgccccgt cagccatctg ctcgcctcgt ccaaggcgtg
      421 cgacgccgtg atggccgtcc gcgttccgcc ggccggcgcc cgcctccgct cgatcctgca
      481 tttcggccag ttcgtccagt cgcacgccct gagcttcttc cacctctctt cgcccgacct
      541 gctgctcggc atggactccg acccggcccg gcgcaacatc ttcggcgtca tcgagaaaca
      601 ccccgagctc gcccgcgagg gcatcgctct gcgcaagttc ggccagcagg tgatcgagtg
      661 gctcgccggc gagcgcgtcc atccggcgtg ggtcgtcccc ggcggcgtca acgcgccgct
      721 caccgccgac gtgcgcgacc gcatcctgcg cgagatcccc tcggcccgcg ccatcacgat
      781 gaagaccctg gcgctgctca agcgggtcgt cgatcagttc accgaggaga tcgagcattt
      841 cggcaaccac ccctcgctct atctcggcct ggtcgatccc cgcggcaacc tggagctcta
      901 cgacggccgc ctgcgcatca tggacgacat gggcaagttc atgaccgacc aggtcgaccc
      961 cgaaacctac caggacctga tcggcgaagc cgtcctgccc acctcctacc tcaaagcccc
     1021 cttctacaag ccgctgggct acccgcgcgg catctatcgc gtcggaccgc tcgcgcgcat
     1081 caacctctgc gaccagtgcg gcacccccgc cgccgacgcc gaactggccg agttccgcca
     1141 gcgcttcggc cgacctgccc gcggcgcctt ccactatcat tacgcccggc tcatcgaggt
     1201 catcttctgt ctggagaaaa tggaggagtt gctcgacaat cccaacgtgc tcgacccgca
     1261 tgtccgcgcc gaggccggcg tcaacgccga ggaaggcatc ggcgtcgccg aggccccccg
     1321 cggcacgctg atccaccact accgcgtcga cccccacggc acgatccggt gggccaacct
     1381 gatcatcgcc accggccaca acaacttggc catcaatcag gccgtcctgc aggtcgcgcg
     1441 ccatttcgtc gacggcaaca agctgcgcga gggtatgctc aatcgcgtcg aggccgtcgt
     1501 ccgctgctac gacccctgcc tgagttgctc gacgcacgcc atcggccaga tgcccctgcg
     1561 cattcagctc gtcggcgccg acggccaggt gctcgacgaa caacaccgct gacgcccgac
     1621 gcccggacga cgcgcatgcc ccgctccacg ctcatcatcg gctacggcaa cacgctgcgc
     1681 ggcgacgacg gcgccggccc ggccgtcgtg gaccgcctgc aacggcgcgg cccccgcccc
     1741 ggcccgcggc tgcgcgcggc tcaccagttg acccccgaac tggccgagga gctcagccgc
     1801 cacgcgcgcg tcgtgttcat cgatgcgcgc gccgccgaac cgcccggcgc gatcgtctgc
     1861 gagccgctcc agccggccgc cgacgccggc ggcgccgccc tcacgcatca gttcagcccg
     1921 gccatgctgc tgctgctcgc ccgccggctc tacggccggc aacccgcggc ctggctgatc
     1981 gggatcaacg gggccgattt cgatccgggc gagggcctct cccccgcggt cgcccgcgcc
     2041 gtcgatctcg tcgccgcccg gtggcaggcc ctcattgcac agacccccaa ggagtcgacg
     2101 ccatgcatga agcgtccctg atggacagcc tgatgttcgc gatcaatcag agtgcgcgcg
     2161 cgcatggcgc gaagcgggtc aaatgggtcc gggtgcgcat cggcgcccac tgtcatctgt
     2221 cggccgcgca cctccagcac cattacgatg aggccgccgc cggcaccgtc gcggaaggcg
     2281 cccagctcga aattatccag gacgacaacg aaacctcccc ccacgccgac atcgtcgtcc
     2341 tcgacagcct cgaaatcgaa accgactagc tggcaacgcc acgctcccga gtggccatcc
     2401 cccttgtcga atcaaaaacc ccataccatt cccaatcggg atcggtatcg cgatcgctat
     2461 cgaattctcc cccgcccttc catccgcttc aatttgtccg attcccgatt ctctgctaga
     2521 ataacggata gtcgcggggc gcccgtcttc cttcgccccg catcccatca tgatcgagga
     2581 cttcgtgcgc atccacacca tccattttca gaccgctcgc cgcccctcac ccggctggct
     2641 ggcgccgttg gtgctcgtgg gcgtgacgct tctcgtcagc ggcgcggtcc tcgtcacgct
     2701 cgcgtgctcc gccgtgttgt tcgccgcgcg cggtctctcg cgcctgatcg gtcggccttt
     2761 cgagaaaatc ctggattccg ccgcgcgcgg caacggcgcc cccgccggcc gcgagacggc
     2821 gcatggccgg cccgcttcct tctccgatcc tgccggcggc gtcgcccccc ggcgcgccgc
     2881 ctcacccgcg ccgcgcacga tcgatctcgc acccgacgcg gccggcgtct ggcgcgaaga
     2941 cgaataaccg gcgcccacac aaaatccgtc cgctccgccg gattccgccc cgacgccggt
     3001 cgccgcccgg ccaaccagcc aacggcctag accgagatcg gctgtcccgt ttcatcgata
     3061 atctggccgc gaaacatctc gcgcagcagc ttcacccgcc gcgcgatttc gccatcgcgc
     3121 gccatcagcg cctgcgcccg ttccttcgcc gtacgccggt cgaccggcgc ccgcgccacg
     3181 ctcgccctgg tcacggcgcg ctcggtcccc gcctccgcaa ttccaatgga attctcctct
     3241 tccaccgcat cattttccat tggcatttcg tcatcgaccg gcggcgcgct gtcggccagg
     3301 gtcggcgccg tccgctgcgc cgcggccgcc ttcgcgcccc cggcggccgt cccttccggc
     3361 gccggcgccg ccgtaagccc ggcgggcgca tccaggtgcg tgattttcat cggccgcccg
     3421 cacagccgcg ccagcgcctg ttccatcacg ccgatgttct cgggattctc cagaatgcgt
     3481 cgcgaaaacc cgtcttcggc tcgccagtag atcttcagcg cggccgcgcc gagctcgcaa
     3541 cccgccgctt tcaccaggta gcgccgcacc agcccgcgcg cgttctcgct gcactgcgcc
     3601 agcaatcgcg ggaggagcgc ttcgggcgcg actatctcgc cgacgctcgc ggcccccgcc
     3661 gcggggctct cctcttcgct ccgcgcggcc gcgacctccg ccgcttccat cggcggggct
     3721 gaatccatga acgccggcgc ccgtcccgcg cccactcccc ggccgccgac cgccgcgcgt
     3781 ggccgccgca actccgccgc ggacgtctcc gccacgctcg ccgccggctc cgccgtcccc
     3841 gtctcggtcg ccgcgtgcgc ctcgcgtcgc gccgggtcgt cgctcccggc cgctgcgctc
     3901 cgtgcggcgg cggcgctcgt cgcgcggccc gctgactcaa acgcctcctc gggcagcgcc
     3961 ccgatccgtt tcacgatctc atcgatcggc accaccggct tgatcgccgc cacccgcaga
     4021 aacgtgaatt caatcaggaa ccgcgcctgc gacgaaccct tgatccgttc ctccagctcg
     4081 aacatttgat tcagaatatt gtacagcgtc gccgggctga tcgcctcggc cagccgccgg
     4141 gcctcgccca gcgcctcgcc ggtcgtcatc accagcttgt cgcccacgcc cgattgcagc
     4201 agcaggatgt cgcgctgata cgccaccagg ctccggatga accgctccag gctgcgcccc
     4261 cgaattacca gatcctcgat caactccagc agcccggcgg cgttcccctc ggccagccac
     4321 tcggtcgcgc gcgccagggc cgactgatcg gctagtccca gcagccgcgc ggccgcttcc
     4381 gcgtccggct tgccctcggt catcgccagc agttggtcat agatcaccag cgcgtcgcgc
     4441 attcccccct cgaccgaccg cgcgatcagg ccgtgcagct cgcgccgccc cgccggctcc
     4501 agcttcaccc cttcgccttc ggccacctgg tccagccgcc gcgcaatgtc ctcggcgttc
     4561 atccgccgga acgcatgcag cgtgcagcgc gaccggatcg tctcgggcac cttctccagt
     4621 tccgtcgtgg cgaagatgaa caccacgaac ggcggcggct cctccagcgt cttcagcagc
     4681 gcgttgaacg cccccgtcga cagcatgtgg acttcatcga tgatgtaaac cttgtagcgc
     4741 gccgcgaacg gcgcctgcac gatccgctcg cgcaggtcgc ggatgttgtc gacgcccgtg
     4801 ttgctcgccg cgtcgatctc gatcacgtcc agatcggtgc cctgcgcgat gtcgatgcag
     4861 tggcggcacg cgccgcacgg ctccggcgtc gggccctgcg cgcagttgat cgccttggcc
     4921 agcagccgcg ccgacgtcgt cttgcccacc ccgcgcggcc ccgccagcag gtgcccgtgc
     4981 gacacccggc cggtccgcag cgcgttctgc agcgacttgg ccaccgcctc ctgacccacc
     5041 agatcggcga atgtctgcgg ccgccatttg cgcgccagtc cctgatatga ttccgtcagc
     5101 gccatccgcc gctctccatt ttcaccaaat cttcgccttg tttataatcc cctccccctc
     5161 cactttcaag cggcttccgg gacccccttc cacacccgcc cctgacaacc gtgtatggcc
     5221 ctgatgtcct ttgtgtccct tccccttccc ctcacagaat aaactgactc aaatccttgt
     5281 tctccatcag cttgctcagc cgttccttga cgtattccgc atcgaccacg acccggccgc
     5341 gggcctcgtc cggcgcgtcg tacgacacat cctccagcac ccgctccagg attccgtgca
     5401 accgccgcgc gccgatgttc tccgactgcg cgttgatcgt cgccgcgctc cgcgccagct
     5461 catcgatcgc gtcgtcggtg aactccagct ccaccccgtc ggtccgcagc agcgccacgt
     5521 actgcttgat cagcgagttc tccggctcca ccaggatccg tttcaggtcc tccacctgca
     5581 gcgcctccag ctccacccgc agcggaaacc ggccctgcag ctccgggatc agatccgacg
     5641 gcttcgtggc gtgaaacgct ccggccgcga tgaacagaat gtgatcggtc cggatcgccc
     5701 cgtgcttcgt gttgacggtc gatccctcga cgatcggcag aatgtcgcgc tgtaccccct
     5761 cgcgcgagac atccggcccc tggccggcat gccgccccgc gatcttgtca atctcgtcca
     5821 cgaagatgat gccgttctgt tcggcccgtt ccagcgcgat cagcttgatc ttctcgctgt
     5881 ccagcagccg ttcgacttcc tcatcgaaca gcaccttgcg cgcctcgcgc accttcatcc
     5941 gcgtcagctt gcgcttctgc ggcatcatct tctccatcat ctcgcgcagg ccgcccagca
     6001 tctgctcatc cacgccgatc atcccgaaca tcccgtcgac gaccggccgg gccgtcacct
     6061 cgaactccac ctcgcggtct tccagccgac cctcgtcaag cagcttctcc aggttgcgcc
     6121 gcagttcctc gccgtcgggc tcctcatcgg cctcgccccc ctccacctgc gcctcggtgc
     6181 gcgtgccgaa cccgcccagg aaccgccgcc gaccgccccc ctgcggaccc ttgtgcagca
     6241 ggtcgatcaa ccgctcgcgc gcccgcttct cggcccgcgg ccgcgccgcc tcgatctgct
     6301 cctcgcgcac catgttcacc gccgtctcga ccagatcgcg caccattgat tccacgtcgc
     6361 gccccacata gccgacctcg gtgtacttgg tcgcctccac cttgacgaac ggcgcgcgca
     6421 ccagccgcgc cagccgccgc gcgatctccg tcttgcccac ccccgtcggc ccgatcatga
     6481 tgatattctt gggcatcacc tcttcctgca gcgcctcgtc caaccgccgc cgccgcgtcc
     6541 ggttgcgcag cgcgatcgcc accacgcgtt tggcctccgc ctgcccgacg atgaacttgt
     6601 ccagctcgct cacgatctgg cgcggcgtca actcgacctg ggtcgtcatt tcactcacgc
     6661 gcagcatcct tcccacgctt caatatcgtc cctcgtcctc gtgaccggct ctcatcgggc
     6721 cacccaggat ctgtccctcg tcctcgtcct cgtaatcgcc cccaacggcc tcccccaaaa
     6781 ttcgactctc gtcctcgtcc tcggctctcg aaaaaccccc atttttcgcg cctcactccc
     6841 caccgtttca tcacccaccc gcggggcgct ccttccccca tcatacgaac caaactcccc
     6901 aatctcaatc tccgtccccc c
//
LOCUS       DAOPCO010000213         6898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_9801_length_6898_cov_5.320912, whole genome shotgun sequence.
ACCESSION   DAOPCO010000213 DAOPCO010000000
VERSION     DAOPCO010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9801_length_6898_cov_5.320912"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..2935
                     /locus_tag="PLS90_13020"
     CDS             257..2935
                     /locus_tag="PLS90_13020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03367.1"
                     /translation="MRTQEGPFSLGTLMAALLLLITGFVVCFYHLGKADFYRDAPARV
                     AANVRQMNLEGVWIPPRAGDPGRVELPPAYPWAVRLASGLRGSVSAFQTRLPSAISGL
                     LLLFLAALWFYGHAVRYTRDDGADAPAEGFSLLAGLMLAASPIFFICARAGTPEALFT
                     LLWAAAAFCWTESLEARRSFYAGRPWRVWIFWGYGLAGLAILVHGLLILPMLWLPYLL
                     AARSYRLRGPDWIHLAGLLLALCIGGAWYVFLARLDPHRAGDLARELLTLRLGREAMP
                     RLGIGHYGLALLVGTFPWLLLALVMAVRVQRKLSRSPTLVMWLWSLVVNLVLLSVLAP
                     HAGAFRLAPALLIVLLAASGLQRWNFENRWAVALRAGLRILIILLIAVGTFLALLLNS
                     TEGLLFFIVIALGWLGWTIYSRRQGIIYSPWNATVRLAAMAVLILLAGETLHLTNWEP
                     RKRYHVDTIAYFNRVQDALRGLRPKAFAVELGAAERKESKTRLFFWGDRTAGLHDFYL
                     DGRATPAHDLAALYARAGESTYLFAEHDLARLLSEPRLVPTVTQPGRRPELPARVMFR
                     VLPDDLAPSTFTLADRAAWQPPVNLAVLGNTGTRADDQEEVGDAVDAVNGRIALDSVL
                     LLGNNIYGPNVFDHLDFIESFEEPYKRLLKRGVLFHALLGHEDQGYRWLQMKYPAFHM
                     REPYYRLLFGGGMVEVWMLDSERLLPQEGGAGSSAQLRWLRETLAASHATWKVVALHE
                     ALMSASPKADVNEQLAAVLLPLFDEHQVDIVAWADGAWYERLRPAGHRPVFLNAGWSG
                     DSDDANFVTDPSLIAANDRHPGFILLQFTPRVALITAITARGDTIDQGELSRAGAVSW
                     LEPVPITKPEPEPVSLPVASDVAPGLSDAPTTGAMP"
     gene            2952..3770
                     /locus_tag="PLS90_13025"
     CDS             2952..3770
                     /locus_tag="PLS90_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPK03368.1"
                     /translation="MPSAGRLSPTVAQVVGGLRQWPAAVVDFLYPPICILCLRRLERG
                     RQSICLACENLLRVADEWRCRRCGAHGSAEMAPAGDRPCRLCPPAGAHYQGVLAVVGY
                     NDASQALVHRFKYHRRLEVGEAIARLMADDLRRPLSQLHGRLDCVAPVPLHWRRRWER
                     GFNQSLLLARAVSQASGIELNPALLRRKRHTRRQALVPRASRAQNVRGAFALAVEPGA
                     LAGRGVLLVDDVVTTGSTIEECARVLRAGGAREVWVACFARAGMATSLERDQIR"
     gene            3940..4176
                     /locus_tag="PLS90_13030"
     CDS             3940..4176
                     /locus_tag="PLS90_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03369.1"
                     /translation="MATTLSLEQILERIAEMKQETSEKDEVIRDQQMQIEELESKIEE
                     LDAHCRDLENKIVELNDKSRQAEELLERLSQVLG"
     gene            complement(4309..4593)
                     /locus_tag="PLS90_13035"
     CDS             complement(4309..4593)
                     /locus_tag="PLS90_13035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPK03370.1"
                     /translation="MAANSRQKKHLVETTGPDVTQSAHELLNVRQQKYLMVNVIARRA
                     RELNKGAKALVEPREGRTITETAMEEVLSDKLLLQRKQKKTTLVSLINNE"
     gene            complement(4599..6539)
                     /gene="dnaK"
                     /locus_tag="PLS90_13040"
     CDS             complement(4599..6539)
                     /gene="dnaK"
                     /locus_tag="PLS90_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK03371.1"
                     /translation="MAKGKVIGIDLGTTNSCVAVVEGGEPVVISNSEGARTTPSIVAF
                     TKDGERLVGQVAKRQAITNPENTVFSIKRFMGRRLNEVSEEQKKVPYKVVAAPNGDAH
                     VDAMGKTYSPPEISAIILQKMRQTAEDYLGQKVDQAVITVPAYFNDAQRQATKDAGRI
                     AGLEVLRIINEPTAASLAYGLDKKKDERIAVYDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGDDFDQVIIDWMADEFKREQGIDLRNDRMALQRLKEEAEKAKIALSSSMSYTL
                     NLPFITADANGPKHLNLELTRAKLEQLCEPLIERTAGPCQKALEDAGFKPSDIDEVIL
                     VGGMTRMPAVQAIVKRIFAKEPNKSINPDEAVALGAAIQGAVLAGEVSDVLLLDVTPL
                     SLGIETLGGVFTRLIERNTTIPTRKSEIFSTASDNQHAVSIHVLQGEREFARDNRTLG
                     RFDLVGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGKDQKIRIEASSGLTEEEIK
                     RMVREAESHADEDRQRREEIATINQADALVYSVEKLLREQGGKLNEADRGEIDRALEA
                     LKKAIADKDVNAIKSGSERLNEATHRLSAKLYEQAQAGQAAAGAQQAAGGFAGEPSAE
                     SEASGNEDVVDAEFEVKDEKKD"
     gene            complement(6577..>6898)
                     /locus_tag="PLS90_13045"
     CDS             complement(6577..>6898)
                     /locus_tag="PLS90_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03372.1"
                     /translation="HADADGGANRDSEYLSQKNGRCFGGRRGCDRCRFHPPAVAWHEL
                     SGALVQRAWDLFRRHQIGTNDAFYCILAGDWGAEIWSQDGNFIDQALKSGINMHDLRA
                     KAFS"
BASE COUNT          978 a         2577 c         2035 g         1308 t
ORIGIN      
        1 cccggacgcg gcgccgtcta tcctataacc cctcatcgtc aatttgccaa ctccgcatca
       61 gcggcctcag ctcccaacaa gcgtcgtccc ttgtcctcgc ccgtgtgatc gacccgtccc
      121 gccctccgct tccgcccccc gccccggctt gacggttctt cacccttcac ggcatactgc
      181 ggggcgagac cggacccgct ccgccggacg gttgctcgcg acgattccca ttttccgttg
      241 aggtcgccgc tccccaatgc gcacgcagga agggccgttt tcgctcggaa cactgatggc
      301 ggccctgctg ctgctgatca ccggcttcgt cgtctgcttc taccacctcg gcaaagccga
      361 cttctaccgg gacgcccccg cccgcgtcgc ggcgaacgtc cgccagatga acctcgaggg
      421 cgtctggatc ccgccgcgcg ccggcgaccc cggccgcgtc gaactccccc cggcctatcc
      481 ctgggccgtg cgccttgcct ccggcctgcg ggggagtgtc tcggcgttcc agacccgcct
      541 gccgtcggcg atcagcggcc tgctcctgct cttcctggcc gcgctctggt tctacggcca
      601 tgccgtgcgc tacacgcgcg acgacggcgc cgacgccccc gccgagggct tttcgctgct
      661 ggccggcctg atgctcgccg cctcgccgat cttcttcatc tgcgcccgcg ccggcacccc
      721 cgaggccctg ttcacgctgc tctgggccgc cgccgccttc tgctggaccg aatcgctcga
      781 ggcccgccgc tcgttctatg ccggccggcc ctggcgcgtc tggatcttct ggggctacgg
      841 cctggccggc ctcgcgatcc tcgtccacgg cctgctcatc ctgccgatgc tgtggcttcc
      901 ctacctgctt gcggcgcgct cctatcgcct gcgcggcccg gactggatcc atctggccgg
      961 cctcctcctc gcgctctgca tcggcggcgc atggtatgta ttcctcgcgc gcctggaccc
     1021 ccaccgggcg ggcgatctgg cgcgcgagct gttgacgctc cgcctcgggc gcgaggccat
     1081 gccccgcctc ggcatcgggc attacgggct cgcgctgctg gtcgggacgt tcccctggct
     1141 tctgctggcg ctcgtcatgg cggtccgcgt ccagcgcaag ctgagccgct cgccgacgct
     1201 cgtgatgtgg ctctggtcgc tcgtcgtcaa cctggtgctc ctgtcggtcc tcgcgccgca
     1261 tgccggcgcg ttccggctcg cccccgcgct gctgatcgtg ttgctcgcgg ccagcggcct
     1321 gcagcgctgg aatttcgaga accgctgggc cgtcgcgctg cgcgcggggc tgcgcatcct
     1381 catcatcctg ctgatcgccg tcggcacatt cctcgccctg ctcctcaatt ccaccgaggg
     1441 cttgctcttc ttcatcgtca tcgccctggg ctggctcggc tggacgatct attcccgccg
     1501 ccaggggatc atctattcgc cctggaacgc gacggttcgc ctcgccgcga tggccgtcct
     1561 gatcctgctc gccggcgaga cgctgcacct gaccaactgg gagccgcgca agcgctacca
     1621 tgtggatacg attgcctact tcaaccgggt gcaggatgcg ctgcgcggac tccggccgaa
     1681 ggcgttcgcc gtcgagctgg gcgccgccga gcgcaaggaa tcgaaaaccc gcctcttctt
     1741 ctggggcgac cgcaccgcgg gcctgcatga tttttacctc gacggccggg cgaccccggc
     1801 ccacgacctg gcggcgcttt acgcgcgggc cggcgaaagc acctacctct tcgccgagca
     1861 tgatctggcg cgcctgctca gcgaaccgcg gctggtgccc accgtcaccc agcccggccg
     1921 ccgtcccgag ctgccagccc gcgtcatgtt ccgcgtcctt cccgacgacc tggcgcccag
     1981 caccttcacg ctcgccgatc gcgccgcctg gcagcccccg gtcaacctcg ccgtcctcgg
     2041 caacaccggc acccgcgccg acgatcagga ggaggtcggc gacgccgtcg acgccgtcaa
     2101 cgggcgcatc gcgctcgatt ccgtcctcct cctgggcaat aacatttatg gccccaacgt
     2161 cttcgatcac ctcgatttca tcgagtcctt tgaggagccc tataagcgcc tgctcaagcg
     2221 cggcgtcctc ttccacgccc tgctcgggca cgaggaccag ggctaccgct ggctccagat
     2281 gaaatacccc gccttccaca tgcgcgagcc ctactaccgg ctgctctttg gcggcggcat
     2341 ggttgaagtc tggatgctcg attccgaacg cctgctgccg caggaaggcg gcgcgggctc
     2401 gtcggcgcaa ctgcgctggc tgcgcgagac gctggccgcc tcccacgcca cctggaaagt
     2461 cgtggcgctg cacgaggcgc tgatgtccgc ctcgcccaag gccgacgtca acgagcagct
     2521 cgccgccgtc cttctgccgc tcttcgacga acaccaggtc gacatcgtcg cctgggccga
     2581 cggcgcgtgg tacgagcggc tccggccggc cggccaccgg cccgtcttcc tcaacgccgg
     2641 ctggagcggc gacagcgacg acgccaactt cgtgaccgac ccgagcctga tcgccgccaa
     2701 cgaccgccat cccggcttca tcctgctgca gttcactccc cgcgtcgccc tgatcaccgc
     2761 catcaccgcc cgcggcgaca cgatcgatca gggcgaactg tcccgcgccg gcgccgtcag
     2821 ttggcttgag ccggtcccga tcaccaaacc cgaacccgaa ccggtcagcc tgccggtcgc
     2881 ctccgatgtc gcccccggcc tgagcgatgc gccgaccacc ggcgccatgc cctgatcccc
     2941 gggagcccgc gatgccctcc gccggccggc tttcccccac cgtggcccaa gtcgtcggcg
     3001 gactgcgcca atggcccgcg gccgtcgtcg atttcctcta cccgccgatc tgcatcctct
     3061 gcctgcgccg gctggagcgc ggccggcaat cgatctgcct cgcttgcgaa aaccttctgc
     3121 gcgtcgccga cgaatggcgc tgccgccgtt gcggcgccca cggcagcgcc gaaatggccc
     3181 ccgccggcga ccgcccctgc cgcctctgcc ccccggcagg cgcacattac cagggcgtcc
     3241 tcgccgtcgt cggctacaac gacgcttccc aggcactggt gcatcgcttc aaataccacc
     3301 gccggctcga agtcggcgag gccatcgccc gcctgatggc cgacgatctg cgtcggccct
     3361 tgagccagtt gcacgggcgg ctggactgcg tcgcgcccgt cccgctccac tggcgccgcc
     3421 gctgggagcg cgggttcaac cagagcctgt tgctggcgcg tgcggtcagc caggcgtcag
     3481 ggatcgagct gaaccccgcc ttgctccgcc gcaagcgtca cacccggcgc caagcccttg
     3541 tgcccagggc ttcccgcgcg cagaatgttc gcggggcctt tgcgctggcc gtcgaacccg
     3601 gcgcgctcgc cgggcgcggc gtcctgctcg tcgacgacgt cgtgacgacc ggctccacaa
     3661 tcgaggagtg cgcccgcgtc ctgcgcgccg gcggcgcgcg cgaggtctgg gtcgcctgct
     3721 tcgcccgcgc cggcatggcg acgtccctcg aacgcgacca gatccgctag accagcgccc
     3781 gccgccgcgt tggcccgatt ctggccagca caacggcctt cggagcaaga tttgtcgccg
     3841 ttattctcaa gtcgctctaa actgattccg ataggaacct gaaggttggc aacggcacgg
     3901 gctgaccgcc gcgtttaatc cctcaggagg agatccctca tggccaccac gctatcgctc
     3961 gagcagatcc tggaacgcat cgccgagatg aagcaggaga cttccgagaa agatgaagtc
     4021 atccgcgatc agcagatgca gatcgaggag ctggagagca agatcgaaga gctcgacgcg
     4081 cactgccgcg atctggaaaa caagatcgtc gaactcaatg acaagtcccg ccaggcggaa
     4141 gagctgttgg agcggctcag ccaggttctg gggtagtccg ccatccggca agcccgcgcc
     4201 aatcgaattt cacccgctgc gatgcaccgg aattgaaaga cccttccccc cgcccgggga
     4261 gaagggtctg tctttattca tcggccccgg ccgcgccgtg ccccgccgtt attcgttgtt
     4321 gatcaggctg accagcgtgg tcttcttctg cttgcgctgc agcagcagct tgtcgctcag
     4381 gacttcctcc atcgccgtct cggtgatggt ccgtccctcg cgcggttcga ccagcgcctt
     4441 ggcccccttg ttcagctcgc gcgcccgacg cgcaatcacg ttgaccatca gatacttctg
     4501 ctggcgcacg ttgagcagct cgtgggcgct ctgcgtcacg tcgggacccg tcgtctcgac
     4561 aaggtgcttt ttctggcggg aattggctgc cacggcgttc agtccttctt ctcgtccttg
     4621 acttcaaatt cggcgtcgac gacgtcctcg ttgccggacg cttcgctttc cgccgacggt
     4681 tcgccggcga aaccgccggc cgcctgttgc gcccccgccg cggcctgccc cgcctgcgcc
     4741 tgctcataca gcttggccga cagccggtgc gtggcctcgt tgaggcgctc ggagccagat
     4801 tttatggcat tgacatcctt gtccgcaatc gctttcttca acgcctccag ggcccggtcg
     4861 atctcgcccc gatcggcctc gttgagcttt ccgccctgct cgcgcagcag cttctccacc
     4921 gaatagacca gcgcgtcggc ctgattgatc gtcgcgatct cctcgcgccg ctgccgatcc
     4981 tcgtcggcgt gactctcggc ctcgcggacc atccgcttga tctcctcctc ggtcagcccg
     5041 ctggaggctt cgatccggat cttctggtcc ttgcccgtgc cgaggtcctt cgccgacaca
     5101 tgcaggatgc cgttggcgtc gatgtcgaac gtcacctcga tctgcggcac gccgcgcggc
     5161 gccggcggaa tgccgaccag gtcgaaccgc cccagcgtcc ggttgtcgcg cgcaaattcg
     5221 cgctcgccct gcagcacgtg gatcgaaacg gcgtgctggt tgtcgctggc cgtcgagaaa
     5281 atctcgctct tgcgcgtcgg aatcgtcgtg ttgcgctcga tcagccgggt gaagacgccc
     5341 cccagcgtct cgataccgag cgatagcggc gtgacatcca gcagcagcac gtcgctgacc
     5401 tcgcccgcca gcaccgcccc ctggatcgcg gcgcccagcg ccaccgcttc gtccgggttg
     5461 atgctcttgt tgggctcctt ggcgaagatc cgcttgacga tcgcctggac cgccggcatc
     5521 cgcgtcatgc cgccgaccag aatcacttcg tcgatgtccg acggcttgaa gccggcatcc
     5581 tccagcgcct tctggcacgg gcccgccgtg cgctcgatca gcggttcgca aagctgctcc
     5641 agcttcgccc gcgtcagttc gaggttcagg tgcttcggac cgttggcgtc ggcggtgatg
     5701 aacggcaggt tcaacgtgta gctcatcgag gacgagagcg cgatcttggc cttctccgcc
     5761 tcctccttga gccgctgcag cgccatccgg tcgttgcgca gatcgatccc ctgctcgcgt
     5821 ttgaactcgt cggccatcca gtcgatgatg acctgatcga aatcgtcgcc ccccaggtgc
     5881 gtatcgccgt tggtcgactt gacctcgaag acgccgtcgc ccagttcaag aatcgaaata
     5941 tcgaacgtgc cgccccccag gtcgtagacc gcgatccgct cgtccttctt cttgtccagg
     6001 ccgtaggcca gcgaggccgc cgtcggctcg ttgatgatgc gcagcacctc cagcccggcg
     6061 atccggccgg catccttcgt cgcctggcgt tgcgcgtcgt tgaagtacgc cggcaccgtg
     6121 atcaccgcct gatcgacctt ctgaccaagg tagtcctcgg ccgtctgccg catcttctgc
     6181 aggatgatcg ccgagatctc cggcggcgaa taggtcttgc ccatcgcgtc gacgtgcgcg
     6241 tcgccgttgg gggcggcgac caccttgtac ggaaccttct tctgctcctc gctgacttcg
     6301 ttcaagcggc gccccatgaa gcgcttgatc gaaaaaaccg tgttctccgg attcgtgatc
     6361 gcctggcgct tggccacttg gcccaccagc cgttcgccat ccttcgtaaa ggcgacgatc
     6421 gacggcgtcg tgcgcgcccc ctcggagttc gagatgacca ccggctcgcc accctccacg
     6481 acggcaacgc aggaattggt cgtccccagg tcaattccaa tcactttgcc ttttgccatt
     6541 atctttgctc cagattagtc ttgcctgctg ctgatctcag gaaaatgcct tggcgcgcaa
     6601 gtcatgcatg ttgattcctg atttcagggc ttgatcgatg aaattcccat cctgagacca
     6661 gatctcggcg ccccagtctc cggccaatat gcagtagaaa gcgtcgttcg tgccgatctg
     6721 atgcctgcgg aatagatccc acgcccgctg aaccaaggcg ccggacagct cgtgccacgc
     6781 cacggcaggg ggatgaaatc ggcaacgatc gcatccacgt cgcccgccga aacatcggcc
     6841 atttttctgc gacagatatt cagaatctcg gttagcccca ccatcggcat cagcgtgt
//
LOCUS       DAOPCO010000214         6773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10018_length_6773_cov_4.866776, whole genome shotgun sequence.
ACCESSION   DAOPCO010000214 DAOPCO010000000
VERSION     DAOPCO010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10018_length_6773_cov_4.866776"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..307)
                     /locus_tag="PLS90_13050"
     tRNA            complement(232..307)
                     /locus_tag="PLS90_13050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Ala,seq:ggc)
     gene            complement(627..1010)
                     /locus_tag="PLS90_13055"
     CDS             complement(627..1010)
                     /locus_tag="PLS90_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPK03373.1"
                     /translation="MRQEFSIERQDEESAAVLVLRGHLTADAAARLLAEDPRPPDSSR
                     PHLIVDLSELDGIDPAGLWALLRMRRDKATAHGRMLIVTPIDPGSRLTLAQAGLAEIM
                     PFCSSLAEALVWLGRRVLSPNAAPS"
     gene            complement(1138..1929)
                     /locus_tag="PLS90_13060"
     CDS             complement(1138..1929)
                     /locus_tag="PLS90_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPK03374.1"
                     /translation="MSRTTPRIPLQPFLGFQLVALTTTRFRELVVARARARATPFAIG
                     YLNAAQVNLAFANAEHARRLAELHALYADGQAIVWASRWLKRPVPERINAGDFTRELI
                     ADCARAGLKLALIGGRPGAEGTPAEVDRAAARFRQWEPELRLVLTHHGYLSPDDARAV
                     RTKIEAADPDLVMLGMGAPRQEALAARWAADGRPRVWWCVGALFEYYAGTRRRAPRWM
                     RRAGLEWLVRLALEPRRLFRRYVIGNPLFVLRVLRRRPPAYRANR"
     gene            2066..3157
                     /gene="amrS"
                     /locus_tag="PLS90_13065"
     CDS             2066..3157
                     /gene="amrS"
                     /locus_tag="PLS90_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPK03375.1"
                     /translation="MPIQLDNDKQFHHPGRWYHRLEDGRVQCDLCPRYCRIKEGARGF
                     CFIRQNTDGGITLTSYGRSSGFCIDPIEKKPLNHFLPGSSVLSFGTAGCNLGCRFCQN
                     WDISKARETERLADKATPEMIARSAREHGCRSVAFTYNDPVIFAEYAIDTAHACHELG
                     VKAVAVTAGYISDRARPEFYAAMDAANVDLKGFTEDFYRKICFAELGPVLDTLHYLRH
                     ETRVWFEITTLLIPGQNDSDAELHAMTEWIAENLGTEVPLHFTAFHPDFKMTDLSRTP
                     AATLTRARGIAQAKGLRFVYTGNVHDRAGGSTYCPGCRRCVIERDWYQLGEYAMRDGA
                     CAHCGTEIAGVFEAAPENWGPRRERVRIA"
     gene            complement(3212..4147)
                     /locus_tag="PLS90_13070"
     CDS             complement(3212..4147)
                     /locus_tag="PLS90_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPK03376.1"
                     /translation="MRMLFLIAMLLTSLATTSAADAPAAPDAAPRRIISLGPDITETV
                     CALGAGKLLVGVSDFCNWPPEVLALPRCGGYLDPNYEVIAALQPDLILAQGRRHRVEQ
                     FADSRGIRVLMLEIDTIDQILSTIERLGVLLQREEEAARLAGRLRAEMERTAARVVAI
                     DPDERPKVFLTVSRRAGSLDQLSTVSGASFLGEILKLAGGRNIFAEIDQPYPQVSKES
                     LLARQPEVILEFMPELQGADESRLSEIRSDWNALPSLPATQHGRIHVITLDHALRPGP
                     RFTEVLDEIQRLLYPELNLAPAGDAPTTETAASAD"
     gene            complement(4218..5813)
                     /locus_tag="PLS90_13075"
     CDS             complement(4218..5813)
                     /locus_tag="PLS90_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03377.1"
                     /translation="MKRRLDWIALPLAGLLCLALTAESRAQVFANVEYSNISPQDPHP
                     TYYYWNEEDYEPVLDPEPDTYYTWYYIGVVDPDARVPDEVEVKRIAVPRSQWLANRKW
                     IIAKLTMFYQLANGLQPTALQDYIAPPEPETPTGEAGEPLLDDQLYTGEAGYPEAGFG
                     PDGMPLAPTDPGALGNTPFPMAPGMTGMPGPGMPGMPAPGMTGMPGMPMPGMPFAGGP
                     GFPGGPGMPGEMPGAPGALPGPGAAPTDGPGAISAPVMPAAPMGPAFPGGFGGFPGGF
                     GGFPGGFGGYAGGYGGGARFDPQAAAEWTFFYDQLVLWQYYCARNLLDDRDDQLVENL
                     TLEEPMTEQDDPMRRLATLKPDALNDPEAAGIGGFINSQTGDFYTQRQQQPAGSQQQL
                     TDNEGNLLVRELFDPLATYQDPTILSRYRSQFLEARDQREQDLYKMFSAMLDDIDGRE
                     VNQELYEQWLEEKRQELIAYADSWRRVRDGEALVVDDTVFVVTEEPMEYPPANAVNIS
                     RGERVTPQDLLNLDGSVRQPQIR"
     gene            complement(5990..>6773)
                     /locus_tag="PLS90_13080"
     CDS             complement(5990..>6773)
                     /locus_tag="PLS90_13080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03378.1"
                     /translation="TTYRFASAADRKAYHSPRLDRIIAVAHAVEAVLVREERIEPERV
                     SVVYGGFDTEAYARPSGAALRAEFGIADETSLVGCIGAFHKKKGHGLLLEAFARVRRD
                     LPQARLVLVGDGNRGPLEKKIERLGLGDAVIFAGFRRDIPDVLAALDLSVNTSTEGEG
                     LTGALRESLAVGTPVVATDVSGNREIVIDGVTGRLVPVNDVDAAAEAIGWALRHPEAM
                     RAMAAEGQRLVWNQCADTVQAERLEAIYHEILEAKRESQPTG"
BASE COUNT         1094 a         2232 c         2326 g         1121 t
ORIGIN      
        1 aagcgacagg caaccgagtg aatgaatcac acaagacgaa acattcagtt tcagtcatca
       61 ttaaaccgaa gaagagcgtt gggaggggaa gagaaggctg taaatgcgag gcctgggggg
      121 tgacttggat ccagaggtga tgtcgtacca tttggagctg ttgacccagg gctgacccca
      181 aaacaaaaac ccccgccgtg cggcgggggg cgatttgttg gcgattgagg atggtggaga
      241 tacggggaat cgaaccccgg acctcttgac tgccagtcaa gcgctctccc agctgagcta
      301 tatccccact cgttgccgac gcgccggagt tgggggcggg ccgaggccaa gcctgggcgc
      361 ttccaaccgc cgggcgcgtt cggtttcaaa tgctaccgga tggcccggcg catgtcaacg
      421 cgaatgcgct gttttggaga ggccagaaat ggaccaaggt tatggaatcc aaagcggcgt
      481 cgtcgcgcgg caagggccgc gctttgccgt cgcactccac acgagttgcc tcgatccccg
      541 ctccctaccc aggtcaagcg catgatcgtg ccggcagcgg tcagcgcggc gcggcggtcg
      601 cgggaacggc ggtgcgtccg ggccgctcag gaaggggcgg cgttggggct caggacgcgg
      661 cggccgagcc aaaccagcgc ctcggccagc gatgaacaga acggcatgat ctcggcgagc
      721 cccgcctggg ccagtgtcag ccgcgagccg gggtcgatcg gcgtaacgat cagcatccgg
      781 ccgtgcgcgg tggccttgtc gcgccgcatc cgcagcaggg cccacaaccc ggccggatcg
      841 atcccgtcca actcactcag gtccacgatc agatggggac gggacgaatc gggcggccgc
      901 gggtcttcgg ccagcagccg ggccgccgcg tcggccgtca gatggccgcg cagcacgagg
      961 accgcggccg actcctcgtc ctggcgctcg atggagaact cctgcctcat ggcggacgtg
     1021 tcgcttccct gcgaaactgg gttacgcctt ttcgttcttg tctatcgtcg cgcgcgcggc
     1081 gacgccttag gcttgttcag gaaatgaatc agtttgcgcg ggcgggccgg cggcggctca
     1141 acggttggcc cggtaggccg ggggccgtct gcgcaggacg cgcaggacga agagtgggtt
     1201 gccgatgaca tagcggcgaa agaggcggcg gggctcgagt gccaggcgga caagccattc
     1261 gaggccggca cggcgcatcc aacgcggcgc gcgccggcgc gtcccggcgt aatactcgaa
     1321 cagggcgccg acgcaccacc agacccgggg ccggccatcc gccgcccagc gcgcagccag
     1381 cgcctcctgg cgcggggcgc ccatgccgag catgaccagg tcgggatcgg ccgcctcgat
     1441 tttggtccgg accgcgcgcg cgtcgtcggg cgagaggtag ccgtgatgcg tcaggacgag
     1501 cctgagttcc ggttcccact ggcggaaccg ggccgccgcg cggtcgacct cggccggcgt
     1561 tccctcggcg cccggccggc cgccgatcag ggccagtttg aggccggcgc gggcgcaatc
     1621 ggcgatcagc tcgcgggtaa agtcgccggc gttgatccgt tcgggtacgg gccgtttgag
     1681 ccagcggctc gcccagacga tcgcctggcc gtcggcatag agggcatgca attcggccag
     1741 ccggcgggcg tgctccgcgt ttgcaaacgc caggttgacc tgcgccgcat tcaggtagcc
     1801 gatggcgaag ggggtggcgc gcgcgcgcgc ccgcgcgacc accaactcgc ggaaccgtgt
     1861 cgtcgtcagc gcgacaagct ggaatcccag gaacggttgc agggggatgc gcggagtcgt
     1921 acgtgacatg gcgtccgtcg tgaagaagcc tttttccggc ggcggctatc gcgcccccgc
     1981 cgcgcatgtt acaatggcgt cagcgcgatg cgaggcgcgg accgacatgg gccggtcccc
     2041 gtctcgaaag gagattcccc cgagcatgcc catccaactc gacaacgaca agcaattcca
     2101 tcatcccggc cgctggtatc accgcctgga ggatggccgc gtgcagtgcg acctgtgccc
     2161 gcgctattgc cgcatcaagg aaggggcacg cgggttctgc ttcatccggc agaataccga
     2221 tggcggcatc acgctgacca gctatggccg cagttcgggg ttctgcatcg atccgatcga
     2281 gaagaaaccg ctcaatcatt ttctgcccgg ttcgtcggtg ttgtccttcg gcaccgccgg
     2341 ctgcaatctg ggctgccggt tctgccagaa ctgggacatc agcaaggcgc gcgagacgga
     2401 gcggctcgcg gacaaggcga cgccggagat gatcgcccgg tccgcgcggg aacacggctg
     2461 ccgttcggtt gccttcacgt ataacgatcc ggtgattttc gccgagtatg ccatcgacac
     2521 ggcgcatgcc tgccatgagc tgggcgtgaa ggccgttgcc gtgacggccg gctacatcag
     2581 tgatcgggcg cgtccggaat tctacgccgc gatggacgcg gcgaatgtcg acctcaaggg
     2641 gttcaccgag gacttctatc gcaagatctg tttcgcggag ctcggcccgg tgctcgacac
     2701 gctccactac ctgcgccatg agacccgcgt ctggtttgag atcacgacgc tgctgattcc
     2761 tgggcagaat gattcggatg ccgaactgca tgcgatgacc gagtggatcg cggagaatct
     2821 ggggacggaa gtgccgctgc acttcacggc gtttcatccc gatttcaaga tgaccgacct
     2881 gtcgcgcacg ccggcggcga cgctgacccg cgcgcgcggc atcgcccagg ccaagggcct
     2941 gcgttttgtc tacacgggca acgtgcacga ccgcgccggc ggcagcacat attgccccgg
     3001 ctgccggcgg tgcgtgatcg agcgcgactg gtatcagttg ggcgaatacg cgatgcggga
     3061 cggcgcctgc gcgcactgcg ggaccgagat tgcgggcgtt ttcgaggccg ccccggaaaa
     3121 ctgggggccc cggcgcgagc gcgtgcggat cgcgtgacag gcggcgactt cgatctttgt
     3181 ggtgggatct ggcgcgggtt gtcgagttcg ctcagtcggc cgacgccgcg gtttcggtcg
     3241 tcggcgcgtc gcccgccggc gccagattca gttccggata gagcagccgc tggatttcgt
     3301 ccaggacctc cgtgaaccgc ggccccggcc gcagcgcatg atccagcgtg atcacgtgga
     3361 tccgcccatg ctgggtcgcc gggagcgagg ggagggcgtt ccaatccgac cggatctcgc
     3421 tcagccggct ttcatcggcg ccctgcaatt cgggcatgaa ttccaggatc acctcgggct
     3481 ggcgggcgag caggctttcc ttggagacct gcgggtaagg ctggtcgatc tccgcaaaga
     3541 tgttccgccc gcccgccagc ttgaggattt cccccagaaa actggcgccc gacacggtcg
     3601 agagctgatc gagagagccg gcgcggcggc tgacggtcag gaagaccttc ggccgctcgt
     3661 cggggtcgat ggcgacgacg cgggccgctg tccgttccat ttcggcgcgc agccggccgg
     3721 ccaggcgcgc cgcctcctct tcgcgttgca gcagcacgcc gagccgctcg atggtcgaga
     3781 ggatctggtc gatcgtatcg atttcgagca ttagcacgcg gatgccgcgg ctgtcggcga
     3841 attgctccac ccggtggcgg cgcccctgcg ccaggatcag gtcgggctgc agcgccgcga
     3901 tcacttcgta gttcggatcg aggtaaccgc cgcagcgcgg cagcgccagc acctcgggcg
     3961 gccagttgca gaagtcgctg acgccgacga ggagcttgcc cgcgcccagg gcgcagacag
     4021 tctccgtgat gtcgggaccc aggctgatga ttcgccgcgg cgcggcgtcg ggagcggccg
     4081 gcgcatcggc cgcgcttgtg gtcgccaggc tggtcaacaa catcgcaatc aggaacagca
     4141 tgcgcatcac cggccatcct ccatgcggaa acgccgccga cgggcggcgg cgtttatgac
     4201 agatatgcaa tgggtggtta tcgaatctgg ggctggcgca ccgagccgtc gaggttcagc
     4261 aaatcctgcg gcgtgacgcg ttcgccgcgc gagatattca ccgcgttggc cgggggatac
     4321 tccatcggct cctcggtgac aacgaagacc gtgtcgtcga ccacgagcgc ttcgccgtcg
     4381 cgcacccggc gccaactgtc ggcgtaggcg atcagttcct ggcgcttctc ctccagccac
     4441 tgctcataga gttcctggtt gacctcgcgc ccgtcgatgt cgtcgagcat tgcgctgaac
     4501 atcttgtaga ggtcctgctc gcgctggtcg cgcgcctcga ggaactgcga ccggtagcgt
     4561 gacaggatgg tggggtcctg gtaggtggcc agcgggtcga acagctcgcg gaccagcagg
     4621 ttgccttcgt tatcggtcag ttgttgctgc gatccggccg gttgctgctg gcgctgggtg
     4681 tagaagtcgc cggtctggga gttgatgaaa ccgccgatgc cggcggcttc gggatcattc
     4741 agcgcgtcgg gcttgagcgt ggccaggcgg cgcatcgggt cgtcctgctc ggtcatcggc
     4801 tcttccagcg tcaggttttc gaccaactgg tcgtcgcggt catcgagtag attgcgcgcg
     4861 cagtagtatt gccacaggac aagctgatcg tagaagaagg tccattcggc cgccgcctgg
     4921 ggatcgaagc gcgcgcctcc gccataaccg ccggcatacc cgccgaaccc accgggaaag
     4981 ccgccgaatc ccccggggaa tccaccgaat ccacccggaa aagcggggcc catcggcgcg
     5041 gccggcatga ccggcgccga gatggccccc gggccatcgg tcggcgcggc gcccggtccc
     5101 ggcagtgcgc ccggcgcgcc aggcatctcg ccgggcatcc ccggtccacc cggaaaacct
     5161 ggaccgcccg caaacggcat gcccggcatg ggcattccgg gcatcccggt catccctggg
     5221 gcgggcatcc cgggcatccc cggcccgggc atgccggtca tgccgggcgc catcgggaag
     5281 ggtgtgttgc ccagagcccc cgggtcggtc ggcgccagcg gcatgccgtc ggggccgaat
     5341 cccgcttcgg ggtagcccgc ttcgccggtg tagagctgat cgtcgagcag cggctcgccg
     5401 gcctcgccgg tcggagtttc cggctcgggc ggcgcgatgt aatcctgcag cgccgtcggt
     5461 tgcaggccat tggccaactg gtagaacatc gtcagtttgg cgatgatcca tttgcggttg
     5521 gccagccact ggctgcgcgg cacggcgatc cgcttgactt ccacttcatc cggcacccgc
     5581 gcatcgggat cgacgacccc gatgtaatac cacgtgtagt aggtgtcggg ttccgggtcg
     5641 agcaccggct cgtaatcttc ctcgttccag tagtaatagg tggggtgcgg atcctggggc
     5701 gagatgttgc tgtactcgac gttggcgaac acctgagcgc gcgattcggc cgtcagcgcc
     5761 aggcagagca acccggcgag cggcagcgcg atccagtcga gacgacgctt catgacgatg
     5821 ggactccttc ccttgagggg gcctgccggg acacaatccc gcgcccagag cgcgagcgcc
     5881 tcgccaccga ccctctcccc ctaatttagg ccccccacgc gcgacggcca agtgaaattg
     5941 tgcgcccgcg aagggttgcc gcgcgcgccg tggaacgggg gccgcggcat cagccggtcg
     6001 gctgggattc gcgcttcgct tccagaattt cgtggtagat cgcttccaac cgttcggctt
     6061 ggaccgtgtc ggcgcactgg ttccagacca ggcgctggcc ctcggccgcc atcgcgcgca
     6121 tcgcctcggg atggcgcaag gcccagccga tggcttcggc cgcggcgtcg acgtcgttca
     6181 ccggcacgag ccggcccgtc acgccgtcga tcacgatctc ccggttgccg gagacatcgg
     6241 tggcgacgac cggcgtgccg accgccagcg actcgcgcag cgcgccggtc aggccctccc
     6301 cctcggtcga ggtattgacc gagagatcga gcgccgccag cacgtccggg atgtcgcggc
     6361 ggaatcccgc gaagatgacc gcgtcgccca agcccagccg ctcgattttc ttttccagcg
     6421 gtccgcggtt gccgtcgccg acgagcacca gccgcgcctg gggcagatcg cgccgcacgc
     6481 gcgcaaacgc ctcgagcagc agcccatgcc ccttcttctt gtgaaaggcg ccgatgcacc
     6541 ccaccaggga ggtctcgtcc gcgatgccga actcggcccg gagcgccgcg ccggacggcc
     6601 gcgcataagc ctccgtgtcg aagccgccgt agacgacgct gacgcgttcc ggctcgatcc
     6661 tttcttcgcg cacgagcacc gcctccaccg catgggccac cgcgatgatc cgatcgagtc
     6721 gtggggaatg atacgccttg cgatccgccg cgctcgcgaa ccggtaggtc gtt
//
LOCUS       DAOPCO010000215         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10026_length_6771_cov_5.413639, whole genome shotgun sequence.
ACCESSION   DAOPCO010000215 DAOPCO010000000
VERSION     DAOPCO010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10026_length_6771_cov_5.413639"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..3075)
                     /locus_tag="PLS90_13085"
     CDS             complement(292..3075)
                     /locus_tag="PLS90_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03379.1"
                     /translation="MQRASRLLLGAGLAVILTAGGAAAELWPTYRQNRQLTGISPLRG
                     GHRFTPGVRWAIDLGGSTQPAETWDIRDVNGDRYLDLLRFQADALVCMDHEGNMLWEA
                     PGLNNPVLIAAHDFAGDGALGLFLYCDTGAGVQYYLVNGANGNAHLLYTRQNAFSEGR
                     RIGRILPGVKGLQLCVWWSGDAAPNGICPSDGYLWSFENGLDQPNLRFHVSPTGVIYA
                     PVHAFADMDNDGRTDMVMVSHEQLWYWDLASGQLEGAMAWSPQIRTYWSFVAALPLRP
                     GQRPALLIINPHIPGVEVISQNGSSATRLWKAVVGGVEDQYQQVVSIDDGAPNPFMDL
                     NGDQSIEIMAAVGNEHGDGKTNLVIFNADNGARLFDQPGYEILAVDDLDGDGTPETIL
                     QQGGALRITNWNGSAFVDRWSDATATPMLTPAPLEQHLTLGLGPRLVRTNCPLWRESE
                     GSNRFLIRFSDGVWNCQLAPGGGLTKIQKILQHAALGNLPDPDPLLNNYQWSGATLSF
                     TVNGEFTASYTRPQHRVFLAPPPLVGILEGKINVIARNHSGLLNAYTPEGTLLRTLIR
                     STPAFPGLNHVSDDAIGQICDVDGDGENELVSTTRFGGVSVVAIDGDGHLKQRYTAPQ
                     GYTEFAMGPLGNLSSNPADGQWLITRWRTNFEAWRIAAINGKTGQQLWMRDDVSGTFF
                     ALIIPTAVTDYNGDGADDLVAAAEGIAQVISVKNNQTLLGPWNITLVVPGHWTAYGTP
                     IVANLLGAGLPRLIFGRSFALTLVTTLAGQQVWHHPLTRDTTARAQPGLADLDGDGLL
                     EVITSQADGLLTAFSASTSDPQKRGSIRWTLQLNGPVGDIATADLDDDGKQEFLIGGG
                     DGQLHAIKEVGGQGTILWSCDFGRRVGSPILADLDEDDVAEILVPVEDGYLYCLESAS
                     SSTAVRRGMWIYH"
     gene            complement(3120..4235)
                     /locus_tag="PLS90_13090"
     CDS             complement(3120..4235)
                     /locus_tag="PLS90_13090"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK03380.1"
                     /translation="MNASVLSESRELMTLVDRFESIRAEREARAEAFADGRRPYLIYQ
                     TPYPRIYGDVRTSEACFARNLQFIRDSLSVPSDHLPALEPWFGTGVIANVYGCPHIWR
                     DGETPCADYRYDRVEEVRGLPVPDWREGEIARLVMDTIRYMKARTGDAIPIYWTDTQS
                     AHDTATLILDASAVFTACLLEPDSIGEFLSGINRVTLEFSQAQAEAIGDALIKPGRIM
                     ASSARFSGITVSDDNLAVGSPETNRRFNLPLDEELGRAMGGIAIHSCGNWTDAMRMAV
                     ELVPSCVAVDCPICPEVDPNPCRPEAVRDVMANTGIPVHVRVTNDQPHMIEAVKRLLH
                     PNLRPVIRILSLDQASAERNYAALDELLSAYYSSGRA"
     gene            complement(4288..5673)
                     /locus_tag="PLS90_13095"
     CDS             complement(4288..5673)
                     /locus_tag="PLS90_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HPK03381.1"
                     /translation="MDLTLGFCPIGKFVFSHEDAMRYKARIAQRLQADQIRYLDIDAV
                     VADGMIRNQADAEMAVEYFRSQRIDGLFTPHCNFGTEGAVGILGRKLGVPLLLWGPRD
                     EAPLPDGSRYRDTLCGLFASSKVLHKLGVPFTYVENCRLEDPAFGAGLDLFVRTMNVV
                     ARMKNLRIGILGNRIDFFWSTIVNENELLERFGVELQPFDLVKIVGLTRDYAKKNRKR
                     YLAERAELGATLNLDMITDDALANLLGLRDVMLDLAAQWNLRAFAVESFMALPRELGV
                     TIGYAMAALYEHGIPVMHETDIHGAISSVMLEAAGLNREPSFLADLTVRHPTHENGLL
                     LWHDAWPLSLRDPEQPASLGTHWIMPDVEPGLTHWKIREGELTISRFDGDHGRYSVTA
                     ATARSIPGPFTQNNYLWVELANWKTFERHFIEGPYMHHAACAYGDHVAVLRECCKYIP
                     HLEFDPVGSRG"
     gene            complement(5737..>6771)
                     /locus_tag="PLS90_13100"
     CDS             complement(5737..>6771)
                     /locus_tag="PLS90_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03382.1"
                     /translation="RDDAVVPAPDAQYVGEVIEQTIATGRRAVHSAVAAECILARADA
                     RGIGGNRHDPAGPCDPEAPVLALRARDGGDWIGLHVVHAMHPTVLHEDSRLVSADYPG
                     QARRLLKDRLGAGLVVVHHMGAGGNQSPRHFVRGNTLAEARRLGEALGTAVLAALERG
                     QTVAQPVLSVRACRLNEPLARRAIPAPAAAHAALAAARARLAALRADGADPAILRTAE
                     CDVFGAEEVMTLVASARSGSLDAAIAAICPPEIQCFTLDGVAYTAWPGEFSVEYALEL
                     RRRAPNTYLITNAGTHLEGYIVTEEEFQAACYEANCAVFSPEAGRQLLAATVELLHCP
                     TPGMTGVATD"
BASE COUNT         1103 a         2183 c         2359 g         1126 t
ORIGIN      
        1 cccgggtcgt tcggatcggt ctttcacggc ctccattttc agcttttatt atcgatcttg
       61 tcatgcccca agttcagatc cgtcgcaggt cgcgaggcca ggcgatgaga tgcggcaagc
      121 cggtcggcat gcccttctgc ttgccaggcg aacgccaaga gatgacgcca gccagttccg
      181 cggactgaac cgatcagcaa gctggagccg cctggactga acctggattt ctgaagcagc
      241 ttgcgattcg gagaaggaca agctttgtaa accggcagtt cggcggaaag actagtggta
      301 aatccacatg ccgcggcgga cggcggtcga agacgaggcc gactccaggc agtagagata
      361 gccgtcctcc accggcacga ggatttcggc gacgtcatct tcgtccaggt cggccaggat
      421 cgggctgccg acgcgccggc cgaagtcgca actccagagg atcgtcccct ggccgccaac
      481 ttccttgatg gcgtggagct ggccgtcgcc gccgccgatc aggaattcct gcttcccgtc
      541 gtcgtccaga tcggccgtgg cgatgtcgcc gaccgggccg ttcagctgga gggtccagcg
      601 gatgctgccc cgcttctgcg gatcggaggt gctcgcgctg aacgccgtca gcaggccatc
      661 ggcctggctg gtgatcacct cgagcaggcc gtcgccatcc agatcggcca gacccggttg
      721 cgcgcgcgcc gtcgtatcgc gggtcaacgg gtgatgccac acctgctggc cggcgagcgt
      781 ggtgacgagc gtcagcgcga acgagcgccc gaagatcagc cgcggcaggc cggcgccgag
      841 caggttggcc acgatcgggg tgccgtaggc ggtccaatgg ccggggacca cgagcgtgat
      901 attccacggg ccgagcagcg tctgattatt cttcacgctg atcacctggg cgatcccctc
      961 ggcggcggcg accaggtcgt cggccccgtc gccgttatag tcagtcacgg cggtggggat
     1021 gatcagggcg aagaacgtgc cgctgacgtc gtcgcgcatc cagagctgct gccctgtttt
     1081 gccgttgatc gcggcgatgc gccaagcctc gaaattcgtc cgccagcggg tgatcagcca
     1141 ttggccgtcg gcggggttgc tgctgaggtt gcccagcgga cccatggcga attcggtata
     1201 gccttgcggc gcggtgtagc gctgcttgag gtggccgtcg ccgtcgatcg cgacgacgct
     1261 gacgccgccg aagcgcgtcg tgctgaccag ttcgttctcc ccgtcgccgt cgacgtcgca
     1321 aatctggccg atcgcgtcgt cggagacatg gttcagcccg gggaacgccg gcgtgctgcg
     1381 gatcagcgtg cgcaagagcg tcccctccgg cgtgtaggcg ttgagcaggc cggaatgatt
     1441 gcgcgcgatg acgttgatct tgccctcgag gatgcccacc agcgggggcg gcgccagaaa
     1501 gacccggtgc tgcgggcgcg tgtagctggc cgtgaactcg ccattgaccg tgaacgagag
     1561 cgtcgcgccg ctccactgat agttgttgag gagcgggtcg ggatcgggca ggttccccag
     1621 ggcggcatgc tgcaggatct tctggatctt ggtcagtccg ccgcccggcg ccagttggca
     1681 gttccagacg ccgtcgctga agcggatgag gaagcggttc gaaccctccg attcgcgcca
     1741 cagcgggcag ttggtgcgga cgaggcgcgg gccgaggccc agggtcaggt gctgctcgag
     1801 gggggccggg gtcagcatcg gcgtcgccgt ggcgtcggac cagcgatcga cgaacgcgct
     1861 gccgttccag ttggtgatgc gcagcgcgcc gccctgttgc aggatcgtct ccggcgtccc
     1921 gtcgccatcc aggtcgtcca cggccaggat ctcatagccg ggctggtcga agaggcgggc
     1981 gccgttgtcg gcgttaaaga taaccagatt ggtttttcca tcgccatgtt cgttgccgac
     2041 ggccgccatg atctcgatcg actggtcacc gttcagatcc atgaacgggt tgggcgcgcc
     2101 gtcgtcgatg ctgaccacct gctgatactg gtcctcgacg ccgccgacga cggccttcca
     2161 gagccgcgtc gccgaactgc cgttctgcga gatgacctcg acccccggga tgtgcggatt
     2221 gataatcagc agcgcggggc gttggcccgg ccgcagcggc agcgccgcca cgaacgacca
     2281 gtaggtgcgg atctgcggtg accacgccat cgccccctcg agctggccgc tcgccagatc
     2341 ccagtaccag agctgctcgt gagagaccat caccatgtcg gtgcggccat cgttgtccat
     2401 gtcggcaaag gcgtggaccg gcgcgtagat cacgccggtc gggctgacat ggaaccgcag
     2461 attgggctga tcgaggccgt tctcgaagct ccacaaatag ccgtcggacg ggcagatgcc
     2521 attgggggca gcatcgccgc tccaccagac gcagagctgg aggcccttca cgccggggag
     2581 gatcctgccg atccgccgcc cctcgctgaa ggcgttctgg cgcgtataga gcaggtgggc
     2641 gttgccgttg gcgccattga ccaggtaata ctgaacgccg gcccccgtat cgcagtagag
     2701 gaagaggccc agggcgccgt cgccggcgaa atcatgggcc gcgatcagca cggggttgtt
     2761 caggccgggc gcctcccaga gcatgttccc ttcgtggtcc atgcagacga gcgcatcggc
     2821 ctgaaagcgc agcagatcga ggtagcgatc gccattgacg tcgcggatat cccaggtttc
     2881 ggcgggctgg gtcgagccgc ccagatcgat cgcccagcgc acgcccgggg tgaaacggtg
     2941 cccgccgcgg aggggcgaaa tgccggtcaa ctggcggttc tggcggtagg tcggccacag
     3001 ctcggcggcc gctccgccgg ccgtcaagat gacggccagc cccgcgccca gcagcaaccg
     3061 agaggcgcgc tgcatgatga aaaacatgat acaaccccga tccccggccg cgtctgccct
     3121 caggcgcgac cggacgaata atatgcggac aacaattcat ccaaggcggc gtagttccgc
     3181 tcggccgatg cctggtccag cgacaggatg cggatcaccg gccgcagatt gggatgcagc
     3241 agacgcttca ccgcctcgat catatggggt tgatcgttcg tcacgcgcac gtgaaccggg
     3301 attcccgtgt tggccatcac gtcgcggact gcctcgggcc ggcaggggtt gggatcgact
     3361 tcggggcaga tcgggcaatc gaccgcgacg caggagggca cgagctcgac ggccatgcgc
     3421 atcgcatcgg tccagttgcc gcaggaatgg atcgcgatgc cgcccatggc ccggcccagt
     3481 tcctcgtcga gcggcagatt gaaccgccgg ttcgtctcag gcgaccccac ggccaggttg
     3541 tcatccgaca cggtgatccc gctgaaacgc gcactggagg ccatgatgcg acccggtttg
     3601 atcagcgcat cgccgatcgc ctcggcctga gcctggctga actcgagcgt cacgcgattg
     3661 atgccgctca ggaactcccc gatggagtcg ggctccagca ggcaggcggt aaagacggcc
     3721 gaggcatcga ggatcagggt cgcggtgtcg tgggccgatt gcgtgtcggt ccaatagatc
     3781 ggaatcgcat cgccggtgcg cgccttcatg tagcggatgg tgtccatcac gagccgggcg
     3841 atctcgccct cgcgccagtc gggcacgggc aagccgcgga cttcctcgac gcggtcgtag
     3901 cggtagtcgg cgcatggcgt ctcgccgtcg cgccagatgt gcgggcagcc gtaaacgttg
     3961 gcgatgacgc cggtgccgaa ccagggctcg agcgccggca agtggtcgct cggcacgctc
     4021 aaggagtccc ggatgaactg cagattgcgc gcgaagcatg cctcgctggt gcggacgtcg
     4081 ccgtagatgc gcggataggg agtctgatag atcagatagg ggcgccgtcc atcggcgaag
     4141 gcctcggccc gggcctcgcg ctcggcccgg atcgactcaa agcggtcgac cagcgtcatg
     4201 agctcgcggc tctccgacaa gacggacgcg ttcatcagcc gatccttccc ccgcaccccg
     4261 agccgggccg acggccccgt ccggccgtca accacgtgaa ccgaccggat cgaactccag
     4321 gtggggaatg tacttgcagc attcgcgcag cacggcgaca tggtcgccgt aggcgcaggc
     4381 cgcatggtgc atgtaggggc cctcgatgaa atggcgctcg aacgtcttcc agttggcgag
     4441 ttccacccac aaatagttgt tctgcgtgaa cgggccgggg atgctgcgcg ccgtcgcggc
     4501 cgtgacgctg tagcgcccat ggtcgccgtc gaagcgcgag atcgtgagct ctccctcgcg
     4561 gatcttccaa tgggtgaggc ccggttcgac gtcgggcatg atccagtggg tgccgaggga
     4621 ggccggttgc tccggatcgc gcaggctgag cggccaggcg tcgtgccaca gcaagaggcc
     4681 gttctcatgg gtcgggtggc gcacggtcag gtcggcgagg aacgagggtt cgcggttcag
     4741 tccggcggcc tccagcatga cgctgctgat cgcgccgtgg atgtcggtct cgtgcatcac
     4801 cgggatgccg tgctcgtaga gcgccgccat cgcgtagccg atggtgacgc ccagttcgcg
     4861 cggcagcgcc atgaacgact cgacggcgaa ggcgcgtagg ttccattgcg ccgccaggtc
     4921 gagcatcacg tcgcgcagcc cgagcaggtt cgccagcgcg tcgtcggtga tcatgtccag
     4981 gttgagcgtg gcgccgagct ccgcccgctc ggccaggtag cgcttgcggt ttttcttcgc
     5041 gtagtcgcgc gtcagcccga cgattttgac gaggtcgaac ggctgcagtt ccacgccgaa
     5101 gcgttcgagc agttcgttct cgttgacgat ggtcgaccag aagaaatcga tgcggttgcc
     5161 gagaatgccg atgcgcaggt tcttcatccg cgcgacgacg ttcatcgtgc gcacgaacag
     5221 atcgaggccc gcgccgaacg caggatcctc cagccggcag ttctcgacgt aggtgaacgg
     5281 gacccccagc ttgtgcagca ccttgctgga ggcgaacagg ccgcagagcg tgtcgcggta
     5341 gcggctgccg tcggggaggg gcgcttcatc gcgcgggccc cagagcagca gcggcacgcc
     5401 cagcttccgc ccgaggatcc ccacggcgcc ttcggtgccg aagttgcagt gcggggtgaa
     5461 caggccgtcg atgcgctggg aacggaagta ttcgaccgcc atctccgcgt ctgcctggtt
     5521 gcggatcatg ccgtcggcca ccaccgcgtc aatgtcgaga tagcggatct gatccgcctg
     5581 cagccgctgc gcgatccgcg ccttgtagcg catcgcgtcc tcgtggctga agacgaattt
     5641 gccgatcggg cagaagccga gcgtcaggtc catggtgcgg ttggctttct atcggcgcgc
     5701 cccgccgggg ggagggggcg ccggggggcg tcggggttaa tcggtcgcca cgccggtcat
     5761 gccgggcgtg gggcaatgca acagctcaac ggtcgcagcg agcagttgcc tgcccgcttc
     5821 cggggaaaag acggcgcaat tggcctcgta gcacgcggcc tgaaactctt cctcggtcac
     5881 aatgtagcct tccagatggg tgccggcatt cgtgatcaga taagtgttgg gggcgcggcg
     5941 ccgcagctcg agggcatatt cgaccgaaaa ttcgccgggc caggcggtgt aggcgacgcc
     6001 gtcgagtgtg aagcattgga tctcgggggg gcagatcgcg gcgatggccg catccagcga
     6061 cccggagcgc gcgctcgcga cgagcgtcat cacctcctcg gcgccgaaga cgtcgcattc
     6121 ggcggtgcgg aggatcgcgg ggtccgcccc atcggcgcgc agggcggcga gccgtgcgcg
     6181 ggccgcggcc aacgcggcgt gggctgcggc cggcgccggg atcgcgcggc gcgccagcgg
     6241 ctcgttcaag cggcaagcgc gcacgctcag caccggctgc gccacggttt gcccgcgctc
     6301 cagcgccgcc agaacggccg ttcccagggc ttcgcccagc cgccgcgcct cggcaagggt
     6361 gttgccccgg acgaaatggc gcggactctg attgcccccc gcgcccatgt gatgcacgac
     6421 aaccagcccc gcgcccagcc ggtccttcag gagtcgacgg gcctggccgg ggtaatccgc
     6481 ggagaccagc cggctgtcct cgtgcagcac ggtcgggtgc atggcgtgga cgacatggag
     6541 gccaatccaa tccccgccgt cgcgggcgcg cagggcgagc accggcgcct cgggatcgca
     6601 cggtccggcc ggatcatggc gattgccgcc gatcccgcgc gcatcggcgc gggccaggat
     6661 gcactcggcg gcgacggcac tgtgcacggc gcggcggccc gtcgcgatcg tttgttcgat
     6721 cacctcgcca acgtattgcg catccggcgc cggcacgacc gcatcgtcgc g
//
LOCUS       DAOPCO010000216         6754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10050_length_6754_cov_5.234363, whole genome shotgun sequence.
ACCESSION   DAOPCO010000216 DAOPCO010000000
VERSION     DAOPCO010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10050_length_6754_cov_5.234363"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLS90_13105"
     CDS             <1..481
                     /locus_tag="PLS90_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HPK03383.1"
                     /translation="FTRDACSFMAWVRVEGADAKAEGPRHLFSAWAAAHDDPLRVTVE
                     GNQIYAAVEAGQVHATRAAPLPPGPWFHVAVVKEGAALILYLNGEPAAAGDIPTGAES
                     AATNFALGFLPEGAGSGHFHGALDDCTLHARALTADEVLKMYRASAVTAAREWVSYR"
     gene            583..2226
                     /locus_tag="PLS90_13110"
     CDS             583..2226
                     /locus_tag="PLS90_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03384.1"
                     /translation="MLNQTKATEPLKVNTSDLDEALMAGIHCLSNITNPDDRHIPYFT
                     CHLAENVRMGFHHRFSVGNVVGRGLFAMLRAEQALEGKLAVDPEVIDRYRNLLLESYR
                     YIGVSPATPAARGEPFNQIWVTNHYCGWRGLMALAMWRGDEEAREILHRSIDDWKRWF
                     VDPGYSWAAFRDHYELEGHPGTGAEETPWPEADAGWSRRVVYEGKSLLEYYLETGYEP
                     ARELGVQLCEHVLEQHLSADGSMDNDHMYLPSMQLSALALCGRITGNREMLERVRLAY
                     EHGMKPVMAPTIGWVPERRLKQSDVGEINNTGEVIEALLNLGAAGWPAYYQDAERFTR
                     AHLLPAQLLDVSWVEPSPENPPDDSRRAVRERVRGAWGFPAPYGIVATREPYFEGAYF
                     MDIVCGGTWSTAAVKAHCVDYRDGVHYVNLLFDRVTPRLAVQSPYPDGDALRVRLAEP
                     GPVHVRLSDWVDRAALHVSGREGPLDFHLDGVYLVIPAPPVDEWITIEFPLAIRESVE
                     RVNGREIRIRWKGDSVLAMDSMGTPYPFFPEFAEAGGTD"
     gene            2269..3237
                     /locus_tag="PLS90_13115"
     CDS             2269..3237
                     /locus_tag="PLS90_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HPK03385.1"
                     /translation="MQNFERKNQRVRQAFRQAMESRPEPPRRRLQFSWSNWGFGMEDL
                     SQSVERLARNGIKWIELHGNRYGPDLGYRAADVRRILSDHGMGCSGICGMFSPECELS
                     SPSGRVRQNAIDYIRRNLELGAEVGASYFLIVPGAVGRPGAVDDREFDRSVETLRLVA
                     DAFAEAGLRGAVEPIRAAEVSLVHTFDEALAYIEAVGHDAIQHINGDIYHMQVEEAHI
                     AERLLEAGSRLTNLHLADSNRRALGEGAMDLDTMIMALYAIGYDQDPACFVTAEPLGP
                     GGDPYPAMYGKTDPAVLDRLVKTTIDTWNERESAVLDALAATHAHA"
     gene            3315..4037
                     /gene="araD"
                     /locus_tag="PLS90_13120"
     CDS             3315..4037
                     /gene="araD"
                     /locus_tag="PLS90_13120"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019105629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HPK03386.1"
                     /translation="MSFQSLQREAFQLNQAIYEHGLALLTWGNASLHDRDRGVMAIKP
                     SGVSAARLKEEDIVVLSIEDGRTLAGALRPSSDTPTHLYLYRAFAGVGAIIHTHSTHA
                     TAWAQAGQAIPCFGTTHADHFNGAVPVTRPMRPDEIQHDYEENTGRVIAECFATHALD
                     AGQMPGVLVHGHGPFAWGEDGRHALDNAVALEEIARLAWYTRAINPAASPIAPYLLEK
                     HFTRKHGPAAYYGQATGGARDS"
     gene            4099..5118
                     /locus_tag="PLS90_13125"
     CDS             4099..5118
                     /locus_tag="PLS90_13125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03387.1"
                     /translation="MTIELAVIGGGRMAVNHHLPALRRLMDSGRIDVRPRALVDSNPE
                     QADKVAGQFGFATTYPSVDALLRDGRPDAALVLTPYWVNETVTAPLLEHGVAVFTEKP
                     PAMSAAGTRRLAELAERAGVANQVGFNRRYWPPIKTALDWLAEHDAPLQAIRATKHRL
                     GRTNEDYAFYTFSHVIDLILAVAGPATSMQCLSAPVPGADSANLFALIRLAGDCSALA
                     SGMPDVGYQQEIYEFHTRRGTVIVDAYWGTDRPAIVREYVDYDLVREVVLPRGTDRPV
                     IDGFLPQLEAFFDALAGAAPASPTLRDCVQTMELLEALRAGRNWSAGERASEEIPDPA
                     LFAPR"
     gene            5115..>6754
                     /locus_tag="PLS90_13130"
     CDS             5115..>6754
                     /locus_tag="PLS90_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03388.1"
                     /translation="MNRPASQAVGAWWLAAALIVSVASAWPAAGADEPAWPARVAVND
                     HDLRRALSAAVHAISHTLDPAGLPYFECHALERPRLAFNPQFSAANVAGRSLYALLRA
                     RAVGIEVDPAIVAQYRQVVLESYRLTRAMPGNPETPGASYNRYHWSNTGAGLRGLVGL
                     VEFENDDTARRYADEAFQLLRMHLVEEGFNWRRYARLTQLDQLDPKFDAARWPETEET
                     VARSGGFILWAAAEYHRLTGSADALAVARGIADLHVRHAAPADGRLAGYDHVFEPAAE
                     MNALARLALITRDIHYMRRARARYDVGVRAIRSANGWMPENLAFRADVGEINNTAELI
                     ETALSLARWGWPQYYQDAERFMRGHLLPAQLLDVSVIPAAADEPPDSELPFAERIRGV
                     WGFPAPYGHVATRNSYLRGAFHLDIVSGGAVGVAEVWKHCVERTTDSQTVNMWFDYED
                     ELIRVREPYAHDGSLSIVMRAPGTLRVRLPHWLDPGSLRVTVSDEPIAFEVAERMLVI
                     RSAPAEREIRVDCAIPYRAERETLNDRSFDLLWHGDRLV"
BASE COUNT         1106 a         2191 c         2355 g         1102 t
ORIGIN      
        1 tttcacgcgc gacgcatgca gcttcatggc ctgggtgcgc gtggaggggg ccgatgccaa
       61 ggcggagggg ccgcgccatc tgttttcggc gtgggccgcc gcgcatgacg atccgctgcg
      121 cgtgacggtt gaaggcaacc agatttacgc cgccgtggag gccggccagg tccatgcgac
      181 ccgcgcggcg ccgctcccgc cggggccctg gttccacgtg gcggtggtca aggagggcgc
      241 cgcgttgatt ctctatctca acggcgaacc ggcggcggcg ggcgacatcc ccacgggcgc
      301 ggagtcggcg gcaacaaact ttgccctggg attcctgccc gagggagcgg gcagcggtca
      361 tttccacggg gcgctggacg attgcacgct ccatgcccgc gcgctgaccg cagatgaggt
      421 cctgaaaatg tatcgcgcat cggccgtgac ggcggcgcgc gaatgggttt cgtaccgatg
      481 aagcggaagc gcgcggatta tttccggctt tggtccgcag ggaaacaggc gagattatgg
      541 cgccggcccg ctcgggccct tgccgtggcc atggtctgca cgatgctgaa ccaaaccaaa
      601 gcgaccgagc cgttgaaagt caataccagc gacctggacg aggcgctgat ggccggcatt
      661 cattgtcttt ccaatattac aaatccggac gatcgacata ttccgtactt tacgtgtcac
      721 ctggcggaga acgtccggat gggatttcat caccggttca gcgtgggcaa cgtcgtggga
      781 cgcggattgt tcgcgatgct gcgcgccgag caggcgctcg agggtaagct cgcggtcgat
      841 ccggaggtga tcgaccgtta tcgcaatctg ctgctggaaa gctaccggta catcggagtc
      901 agcccggcca cgcccgccgc caggggggaa ccgttcaatc agatctgggt caccaatcac
      961 tactgcggct ggcgcggcct gatggcgctg gcgatgtggc gcggcgacga ggaagcccgg
     1021 gaaatcctgc accggagcat cgacgactgg aaacgctggt ttgtggatcc gggctacagt
     1081 tgggcggcgt ttcgcgacca ctatgagctg gaggggcacc ccggcaccgg cgccgaggag
     1141 acgccgtggc ccgaggccga tgcgggctgg agccggcgcg tcgtctacga aggcaagagt
     1201 ctcttggaat attatctgga aacggggtac gagccggcgc gcgagctggg cgttcaactc
     1261 tgcgagcatg tgctggagca acatttatcc gccgatggct cgatggataa cgaccacatg
     1321 tacctgccga gcatgcagct cagcgcgctc gcgctctgcg gccggatcac gggcaaccgg
     1381 gagatgctag agcgcgtgcg gctggcctat gagcacggga tgaagccggt gatggcgccg
     1441 acaatcggct gggtgccgga acggcgcctg aagcagagcg acgtcggcga gatcaacaat
     1501 acgggggagg tgatcgaggc gctgctcaac ctgggggcgg cgggctggcc cgcatactat
     1561 caggatgccg aacgattcac gcgcgcgcac ctgctgcccg cgcaactgct ggacgtgtcg
     1621 tgggtggaac cgagccccga gaacccgccg gacgacagcc ggcgcgccgt gcgcgagcgc
     1681 gtgcgcggcg catggggctt cccggcgccc tacggcatcg tcgccacgcg cgaaccgtat
     1741 ttcgaggggg cctatttcat ggacatcgtc tgcggcggaa cgtggtccac ggcggcggtc
     1801 aaggcgcatt gtgtcgacta tcgcgacggc gtgcattacg ttaatctgct attcgaccgc
     1861 gtcacgccgc gcctcgcggt ccagagcccc taccccgacg gcgacgcgtt gcgcgttcgc
     1921 ctcgccgagc cgggccccgt tcacgttcgg ctgtcggact gggtcgatcg cgccgcgctc
     1981 catgtttcgg gccgcgaagg gcccctcgat tttcatctgg acggcgtcta tctggtcatt
     2041 ccggcgccgc cggtggatga atggatcacg attgaatttc cgctggccat ccgcgagtcg
     2101 gtggagcggg tcaacgggcg cgagatccgg atccgctgga agggcgattc ggtgctggcg
     2161 atggactcga tgggaacgcc atatcccttc tttccggagt tcgccgaggc cggcggaaca
     2221 gactgagcga gatctggctt tcaaccgttt cccaaggagg aaccgacgat gcagaacttc
     2281 gaacgcaaaa accagcgggt gcggcaggcg ttccgccagg cgatggagtc gcgccccgaa
     2341 ccgccgcggc ggcgcctcca gttttcctgg tcgaactggg ggttcggcat ggaggacctg
     2401 agccagtcgg tcgagcgcct ggcgcgcaac gggatcaagt ggatcgagct gcacggcaat
     2461 cgctacggcc ccgatctggg ctatcgggcc gccgacgtgc ggcgcattct gtccgatcac
     2521 ggcatgggct gctcgggcat ctgcgggatg ttcagcccgg agtgcgagct gtccagcccg
     2581 agcggccgtg tgcgccagaa cgcgatcgac tatatccggc gcaatctgga gctgggcgcg
     2641 gaagtcggcg cgagttattt tctgatcgtc cccggcgcgg tcggccgccc cggcgccgtc
     2701 gatgaccgtg aattcgaccg ctcggtggag acgttgcggc tggtggccga cgcgttcgcc
     2761 gaggcgggcc tccgcggcgc cgttgaaccg attcgcgccg ccgaggtcag cctggtccat
     2821 acgttcgacg aggcgctggc ctacatcgag gcggtcggcc acgacgcgat tcagcatatc
     2881 aacggcgaca tctaccacat gcaggtggag gaggcgcata tcgcggagcg gctgctcgag
     2941 gcgggctcgc ggctgaccaa tctgcatctg gccgacagca atcgccgcgc cctgggcgaa
     3001 ggcgcgatgg atctggacac aatgatcatg gcgctctacg ccatcggcta cgatcaggat
     3061 ccggcgtgct tcgtcaccgc cgaaccgctc ggaccgggcg gcgaccccta cccggcgatg
     3121 tatggcaaga ccgatccggc ggttctcgac cgcctggtca agacgacgat cgacacgtgg
     3181 aacgaacggg aaagcgccgt gctggatgct ctggcggcga cccacgccca cgcttgatca
     3241 ggcgtttatt ggaagccccg gcggcgcgaa cggcgcccgc gggcggatcg cgcaatcggg
     3301 aggtgtccgg cagcatgtcg ttccaatcgc ttcaacgcga ggcatttcaa ctcaatcagg
     3361 ccatctacga gcatgggctg gcgctgctga cgtggggcaa cgccagcctg catgatcgcg
     3421 accgcggggt gatggcgatc aagccgagcg gcgtgagcgc ggcccgattg aaggaggagg
     3481 atatcgtcgt cctcagcatc gaggacggcc ggacgctggc cggcgcgctg cgcccttcgt
     3541 ccgacacgcc gacgcacctc tatctctacc gcgcgttcgc gggcgtcggg gcgatcattc
     3601 atacgcattc gacgcacgcg accgcctggg cgcaggccgg ccaggccatc ccctgctttg
     3661 gcacgacgca tgccgatcat ttcaacggcg cggtgcccgt gacgcggccg atgcggccgg
     3721 acgagattca gcatgattac gaggagaaca ccggccgggt gatcgcggaa tgctttgcaa
     3781 cgcatgcgct cgatgccggc cagatgccgg gcgtgctggt gcacggccat ggcccgttcg
     3841 cctggggcga ggacggccgg cacgcgctgg acaacgcggt cgcgctggag gagatcgcgc
     3901 ggctggcgtg gtatacgcgc gcgatcaatc cggccgcgtc gccaatcgcg ccctatctgc
     3961 tggagaagca cttcacacgc aaacacggcc ccgcggcgta ctacggccag gccacgggcg
     4021 gagcgaggga ttcttgagcg gggccttcgg ggacgaggac gaggaacgat ttccaacaga
     4081 cgcacgggag aaagcaggat gacgattgaa ctggcggtga ttggcggcgg gcggatggcg
     4141 gtgaaccacc atctgccggc gctgaggcga ttgatggatt cgggacggat cgatgtccgt
     4201 ccgcgggcgc tggtcgacag caacccggag caggccgaca aagtcgccgg ccaattcggc
     4261 ttcgcgacaa cctatccctc ggttgacgcg ctgctgcgcg acgggcggcc cgatgccgcg
     4321 ctagtgctga ccccctattg ggtcaatgag accgtcaccg cgccattgct ggaacacggc
     4381 gtcgccgtgt tcaccgagaa gccgccggcg atgtcggccg ccggcacgcg gcggctggcg
     4441 gaactggccg aacgcgccgg cgtcgcgaac caggtcggct tcaaccggcg ctactggccg
     4501 ccgattaaga ccgcactgga ctggctggcc gaacacgacg cgccgctcca ggccatccgc
     4561 gccaccaagc accgcctggg ccggaccaac gaagactacg cattttacac gttctcgcat
     4621 gtgatcgacc tgatcctggc cgtcgccgga ccggcgactt cgatgcaatg cctgtcggcg
     4681 ccggtgcccg gcgccgacag cgccaacctg ttcgcgctga tccggctggc gggcgactgt
     4741 tcggcgctgg cctcgggtat gcccgacgtc ggctatcagc aggagatcta cgaatttcac
     4801 acgcggcgcg gcacggtgat cgtcgatgcc tactggggca ccgaccggcc ggcgatcgtg
     4861 cgtgaatatg tcgattatga cctggtgcgc gaggtggtgc tgccgcgcgg cacggatcgg
     4921 ccggtgatcg acggctttct gccgcaactg gaggcgttct tcgacgcgct cgcgggcgcc
     4981 gcgccggcct cgccaacgct gcgcgattgc gtgcagacaa tggaactgct cgaggcgctt
     5041 cgcgcggggc gcaactggtc ggcgggggag cgcgcctcgg aggaaatccc cgaccccgcg
     5101 ctgttcgcgc cacgatgaac cggccggcca gccaggcggt gggcgcgtgg tggcttgccg
     5161 cggccctgat cgtgagcgtc gcgtcagcat ggccggccgc gggcgccgac gaacccgcgt
     5221 ggcccgcgcg cgttgcggtc aacgaccacg atttgcgccg cgcgctctcg gccgccgtgc
     5281 atgccatctc gcatacgctc gaccccgccg gtctacccta tttcgaatgc cacgcgctgg
     5341 agcggccgcg gctggcgttc aacccgcaat tctccgcggc gaacgtggcg ggacgttcgc
     5401 tctacgcctt gctccgcgcg cgggccgtcg gcatcgaggt cgacccggcc atcgtggcgc
     5461 aataccggca ggtcgttttg gaatcctacc ggctgacgcg cgcgatgccg ggcaatcccg
     5521 aaacccccgg cgcctcgtac aaccgctacc actggtcgaa caccggcgcg ggcctgcgcg
     5581 gcctggtggg gctggtggag ttcgagaacg acgacacggc gcggcggtac gccgacgagg
     5641 cgttccagct gttgcggatg cacctggtgg aggagggctt caactggcgg cgctacgcgc
     5701 ggctcacgca gctcgatcag ctcgacccga aattcgacgc cgcgcgctgg cccgaaacgg
     5761 aagagacggt cgcccgctcc ggcggcttca tcctgtgggc cgcggccgaa taccatcggc
     5821 tgaccggttc ggccgacgcg ctggcggtcg cgcggggaat cgccgatctg cacgtgcgcc
     5881 acgcggcgcc ggccgacggc cggctggcgg gctacgatca tgttttcgag ccggcggcgg
     5941 aaatgaacgc cctggcgcgg ctggcgctca tcacccggga catccactac atgcggcgcg
     6001 cccgcgcgcg ctacgacgtc ggcgtgcgcg cgatccgctc ggcgaacggc tggatgccgg
     6061 agaacctggc gtttcgcgcc gacgtgggcg agatcaacaa caccgccgaa ctgatcgaaa
     6121 cggcgctctc gctggcgcgc tggggctggc cgcaatatta tcaggatgcc gaacgattca
     6181 tgcggggcca tctgctgccg gcgcagctgc tcgatgtctc cgtaattccc gccgccgcgg
     6241 acgagcctcc cgattccgag cttccgttcg cggaacgcat ccggggcgtg tggggcttcc
     6301 ccgcgccgta tggtcacgtc gccacgcgca acagctatct gcgcggggcg ttccacctcg
     6361 atatcgtcag cggcggcgcc gtcggcgtcg cggaggtctg gaagcactgc gtcgaacgaa
     6421 cgaccgattc ccagaccgtc aatatgtggt tcgactacga ggatgagctg atccgcgtgc
     6481 gcgagccgta tgcccacgac ggttcgctca gcatcgtgat gcgcgcgccg gggacgctgc
     6541 gcgtgcggct gccccactgg ctcgatccgg gctcactccg cgtcacggtg agcgatgagc
     6601 cgatcgcgtt tgaggtcgcc gagcggatgc tcgtcattcg ctccgcgccg gccgagcgtg
     6661 aaattcgcgt cgattgcgcc atcccgtatc gtgccgagcg cgaaacactc aacgaccgtt
     6721 cgttcgacct gctctggcac ggcgaccggc tggt
//
LOCUS       DAOPCO010000217         6749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10061_length_6749_cov_5.420376, whole genome shotgun sequence.
ACCESSION   DAOPCO010000217 DAOPCO010000000
VERSION     DAOPCO010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10061_length_6749_cov_5.420376"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            568..2685
                     /gene="glgX"
                     /locus_tag="PLS90_13135"
     CDS             568..2685
                     /gene="glgX"
                     /locus_tag="PLS90_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HPK03389.1"
                     /translation="MSKLSLLPGKPYPLGATYDGHGTNFAIFSEIAEGVDLCLIDEEG
                     CETRFEMYDSVAHCWCGYVKTVGAGQRYGYRIHGPWDPVQGHRCLHSKRLLDPYAKAI
                     SGQVQWDPAVFPYHFGDPYGAPNTADSAPFVPCSVVINPAFAWGDDLRPEIPLHETII
                     YELHVKGFTKRHPDVPEEMRGTYAGLSHPAAVDYLKRLGVTTVELMPVHQFVHDAHLL
                     ERGLRNYWGYNSIGFFAPHNEYAAGGQLGEQVNEFKQMVKALHAAGIEVILDVVYNHT
                     AEGNHLGPILSFKGIDNAAYYRLVCDDACHYMDYTGTGNTLNMRNPPVLQLLMDSLRY
                     WVLEMHVDGFRFDLAAALARGLHEVERLVSFFDVIHQDPVISQVKLIAEPWDVGEGGY
                     QVGNFPVLWSEWNGAYRDTMRDYWRGEPAALGDFARRFAGSADLYEDTGRRPFASINF
                     VTAHDGFTLRDLVSYNDRHNEANGEENRDGSVDNRSFNLGAEGPTNDPGIQEVRRRQQ
                     RNFLASLLLSQGVPMILGGDEIGRTQQGNNNAYCQDNEISWLDWENMDQDLFAFVQRL
                     IHIRREHPTLRRWKWLSGEPVGRGRRGDIVWFRPNGEEMNDEDWDNGFARSLLVFLNG
                     SQIADRDAAGGFITDHDFYLLFNAHIEPVEFELPPDDQRKTWARYIDTSDPGQGEGKQ
                     DINARDRIAVDARTLLVLRHVKP"
     gene            2712..3194
                     /locus_tag="PLS90_13140"
     CDS             2712..3194
                     /locus_tag="PLS90_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK03390.1"
                     /translation="MRIQDVMTREVSCCRPGDSLSEAARCMWECDCGATPIIDEERRV
                     VGILTDRDICMAAYTRGAPLHAIEIASVMSTDLCACRPGDALEDVETQMRQHRVRRIP
                     VVDEQDRLVGIVSLNDLALEFQKEQGQRRKSIKAREVAETLAAICEHQHQPALGAAAG
                     "
     gene            3199..4725
                     /locus_tag="PLS90_13145"
     CDS             3199..4725
                     /locus_tag="PLS90_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK03391.1"
                     /translation="MQASRNLSTSPGSCWRREPRALPVCPPAAGELETDVVILGAGIT
                     GLTAGLHLLERGRRVMLLEAHQVGGGATGGSTGHLTALLDTGLGRLIEDWGQHEAGLM
                     VGANLTAISQIEQWSARFSPDCQLQRVEGILYTENAAGAGDLGREADAARLLGLGAEA
                     LDRVPLPFPCHAGYRITGQARFDPLRYVLGLARRFLEAGGVIHEQSPAKPPQDGGGAQ
                     IETPAARIQAGDVIVATHLPYVGALTLETRNYPQQSYVLLVRLTSPLHDALFWDDAEP
                     YHYIRRYGGDDPHLVLIGGADHRTGLPINELDHLAQLEQYARARFAVEAVEGRWSDEF
                     VEPADGAPFAGRLVGADHIWIATGYAGNGLTWGTACGLLLADLLTNEPNPLAGLLDPR
                     RVKGLAALDRFVPENLRTALEYARIPFEGERVDSLAEVGPGEGRLVRYQGRKLAVWRD
                     PGGELTILSPVCRHLGCIVKWNEAAATWDCPCHGGRYTATGERLGGPPPDDLEPAALE
                     "
     gene            4947..5171
                     /locus_tag="PLS90_13150"
     CDS             4947..5171
                     /locus_tag="PLS90_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03392.1"
                     /translation="METTRIATLENEVEAALLGDILREREIPHFIQSFRDAAYDGIFQ
                     TQKGWGCVVGPPERADEVREILEDLRKQPG"
     gene            complement(5229..6611)
                     /gene="thrC"
                     /locus_tag="PLS90_13155"
     CDS             complement(5229..6611)
                     /gene="thrC"
                     /locus_tag="PLS90_13155"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPK03393.1"
                     /translation="MAPLTFHSTNGQAPRVDLKQALLAGQAPDRGLYLPDRIPELSAD
                     RIADLRGKPYADVAFDVLSAYTDGVIEPEALRALCRDAYDYAVPLEPVAGVERVHVLR
                     LDQGPTASFKDFAARMMARLIGRFLQEDRRELLILTATSGDTGSAVASAFHNVPGVRV
                     LVLFPREEVSERQRRQMTTLGDNIRTIALEGKFDDCQALVKRAFSDPDLADLDLSSAN
                     SINIGRLLPQTVYYFYAAAQVAEPDEPVVVAVPSGNFGNMMGCMLAGRMGLKLKKLVV
                     PVNANDEFPVFLRTGRYARIEPSRACISNAMNVGHPSNLARLVELYGGWLDETGCLHR
                     APDFAAMRRDLFSVAVSDDATRAAIRQAWRDHRLLLEPHGAVGWQGLLEYRRAEGLDG
                     APAVVVETAHPAKFPDEIERLLGFTPEVPGALARLEGLPEDYDALPPRYELLKDYLLE
                     RYGGARASAH"
BASE COUNT         1146 a         2232 c         2288 g         1083 t
ORIGIN      
        1 atggttcttc ttttttatgc gccaccctct gagccgttat gaaggattaa ggtgaacaaa
       61 aattttggcc ccggcggagg cggcgcctcc cgccggaccc gcgaaaaccc ggagatgcgg
      121 acagggaaaa aggacgtaag ggacataaaa gacgacagcg ggaaagggag cgcaagagag
      181 aggacatagt caacgtttcg gcatggaggc tcaccacata agccttttcc gaagatgacc
      241 acgttgcgcg acgcaggttc ccgttgccgc gcgcgagaat tgcctcggcg ggcggggcga
      301 ttgggacctc ctcacgattt ggcaccgggc ttgcatttgc ccgcggcggg ctgggcgcgc
      361 gcgccaggcg gccgcccacc cgcgaggtta agtcgttccc gatcaatccc gcgggcgacg
      421 gatcagatcg cccgcgcgca ccgctttttg ccgatggcgc agcaagactt acatcgcggc
      481 ggccggcgcg ccgctccggc ggcagccaac cacccgccaa ttcacgacga agctcgggga
      541 tcccagcatg caggccttgg tatttcgatg agcaaattga gccttcttcc cggcaagccg
      601 tatcccctgg gggcgaccta tgacggccat ggcaccaact tcgccatttt ctccgagatc
      661 gccgaagggg tggacttgtg cctgatcgac gaggaggggt gcgagacgcg ctttgagatg
      721 tacgacagcg tcgcccattg ctggtgcggt tatgtgaaga cggtcggggc cggtcagcgt
      781 tacggctatc ggattcacgg cccgtgggat ccggtgcagg ggcaccgctg cctccactcc
      841 aagcggctgc tcgatcccta cgccaaggcg atcagcgggc aggttcaatg ggatccggcg
      901 gtctttccct accacttcgg cgatccgtac ggcgcgccga acaccgccga cagcgcgccg
      961 ttcgtgccgt gttcggtcgt gatcaacccg gcgtttgcgt ggggcgacga tctgcgcccc
     1021 gagatcccgc tgcacgaaac gatcatctac gagttgcacg tcaagggctt cacaaagcgc
     1081 catcccgacg tgccggagga gatgcgcggg acgtacgccg gcctgtcgca tccggccgcc
     1141 gtggattatc tgaagcgcct gggcgtgacg accgtggaac tgatgccggt ccaccagttc
     1201 gtgcacgacg cgcacctgct cgagcgcggg ctgcgcaatt actggggcta caactcgatc
     1261 ggctttttcg cgccgcacaa cgaatatgcc gccggcggcc aactgggcga gcaggtcaac
     1321 gagttcaaac agatggtcaa ggcgctgcac gccgccggaa tcgaagtcat cctcgacgtc
     1381 gtctacaacc acaccgccga aggcaaccac ctggggccga tcctctcgtt caaggggatc
     1441 gacaacgccg cctactaccg gctcgtctgc gacgatgcct gccattacat ggactacacg
     1501 gggaccggca acacgctcaa catgcgcaac ccgccggtgc tgcagctgct gatggattcg
     1561 ctgcgctact gggtgctgga gatgcacgtc gacggttttc gtttcgatct ggcggccgcg
     1621 ctggcgcgcg gacttcacga ggtggagcgg ctcgtctcgt tcttcgacgt gattcatcag
     1681 gaccccgtca tcagccaggt caagctgatc gccgaaccat gggacgtcgg cgaggggggc
     1741 tatcaggtcg gcaacttccc cgttttgtgg tcggagtgga acggcgctta tcgcgacacg
     1801 atgcgcgact actggcgcgg cgagccggcc gcgctgggcg acttcgcgcg ccgcttcgcc
     1861 ggcagcgccg atctgtacga agataccggc cgtcggccgt tcgcctcgat caactttgtc
     1921 acggcgcacg atggcttcac gttgcgcgac ctcgtctcgt acaacgaccg gcacaacgag
     1981 gccaacggcg aggagaatcg cgacggcagc gttgacaacc ggtcgttcaa tctcggcgcg
     2041 gagggcccga ccaatgaccc cgggatccag gaagtgcggc gccgccagca gcgcaacttc
     2101 ctggcttcgc tcctcctgtc gcagggggtg ccgatgatcc tggggggcga tgagatcggc
     2161 cgcacgcagc agggcaacaa caatgcctat tgccaggaca acgaaatctc gtggctggac
     2221 tgggagaaca tggatcagga cctgttcgcg ttcgtccagc ggctgattca catccgccgc
     2281 gagcatccga ccctgcgccg ctggaaatgg ctgagcggcg aaccggtcgg ccgcggccgg
     2341 cgcggcgaca tcgtctggtt tcgccccaac ggcgaggaga tgaacgacga ggactgggac
     2401 aacggcttcg cccgatcgct gctggtgttt ctcaacggca gtcagatcgc cgaccgcgac
     2461 gccgccggcg gtttcatcac cgaccacgat ttctatctgc tgttcaacgc ccatatcgag
     2521 ccggtggagt tcgagctgcc gcccgacgac cagcgcaaga cgtgggcgcg ctacatcgac
     2581 acgagcgatc cggggcaggg cgaggggaaa caggatatca acgctcgtga ccggattgcg
     2641 gtcgacgcca ggaccctgct ggtcctgcga cacgtcaagc cctgaatgcc agaaacgaaa
     2701 ggatacgcat catgagaatc caggacgtaa tgacgcgcga agtcagttgc tgccgccccg
     2761 gcgacagcct gagcgaggcg gcccgctgca tgtgggagtg cgactgcggc gcgacgccga
     2821 tcatcgacga ggagcgccgg gtcgtcggca tcctcaccga ccgcgacatc tgcatggccg
     2881 cctatacgcg cggcgcgccg ctgcacgcca tcgagattgc cagcgtgatg tcgaccgacc
     2941 tgtgcgcctg ccgtcccggg gacgcgctcg aagatgtgga gacgcagatg cgccagcacc
     3001 gggtccgccg gattccagtg gtcgacgagc aggaccggct ggtcgggatc gtctcgctca
     3061 acgacctggc gctggaattc cagaaggaac aggggcagcg gcggaagtcg atcaaggcgc
     3121 gcgaggtggc cgaaacgctc gccgcgatct gcgagcatca gcaccagccg gcgctcggcg
     3181 cggcggcggg gtaacctcat gcaagcctcg cggaacctct ccacttcgcc cggctcgtgc
     3241 tggcggcgcg agccgcgtgc gctgccggtc tgcccgccgg ccgcgggcga gctggaaacc
     3301 gatgtcgtca tcctgggcgc cggcatcacc gggctcaccg ccgggctcca tctgctggag
     3361 cgcggccggc gcgtcatgct tctggaggcg catcaggtcg gcggcggcgc caccggcggc
     3421 tccaccggcc acctgaccgc cctgctcgat accggcctcg gccggctgat cgaggactgg
     3481 ggccagcacg aggccggcct gatggtgggg gccaacctga ccgcgatcag ccagatcgag
     3541 caatggagcg cccggttcag cccggattgc cagctccagc gggtcgaggg gatcctctat
     3601 accgagaatg ccgcgggcgc cggcgatctg gggcgggagg ccgacgccgc ccggctgctc
     3661 ggtctcggcg cggaggcgct cgatcgggtg ccgctgccgt tcccctgtca cgccggctac
     3721 cggattaccg ggcaggcccg cttcgatccg ctgcggtatg tgctgggcct ggcgcgccgg
     3781 ttcctcgagg ccggcggcgt gatccatgaa cagtcgccgg ccaaaccgcc ccaggacggc
     3841 gggggggccc agatcgaaac gcccgcggcg cggatccagg ccggcgatgt gatcgtcgcc
     3901 acgcatctgc cgtacgtcgg cgcgctgacg ctcgagacgc gcaactatcc gcagcagtcg
     3961 tatgttctgc tggtccggct cacgtcgccg ctgcacgacg ccctcttctg ggacgacgcc
     4021 gagccttacc attacattcg ccgctacggc ggcgacgacc cgcatctggt cctgatcggc
     4081 ggggccgatc atcgcaccgg cctgccgatt aatgaactgg atcatctggc ccagctcgaa
     4141 cagtatgccc gtgcccggtt cgcggtcgag gccgtcgagg ggcgctggtc cgatgagttc
     4201 gttgagccgg ccgacggcgc gccgttcgcc ggccggctcg tcggggcgga tcatatctgg
     4261 atcgccacgg gatatgccgg caacggcctg acgtggggca ccgcctgcgg cctgctgctg
     4321 gccgatctgc tgaccaacga gcccaacccg ctggccggcc tgctcgatcc gcgccgcgtc
     4381 aagggcctcg ccgcgctgga tcggttcgtt cccgaaaatc tgcggaccgc gctggaatat
     4441 gcgcggattc cttttgaggg ggagcgcgtc gacagcctgg cggaggtcgg ccccggcgag
     4501 gggcggctcg tgcgctatca ggggcggaaa ctggccgtct ggcgcgatcc cggcggcgag
     4561 ctgaccatcc tttcgcccgt ctgccggcac ctgggctgca tcgtcaagtg gaatgaagcc
     4621 gcggcgacct gggactgccc gtgccacggg ggccgctaca ccgccaccgg cgagcgtctt
     4681 ggcggcccgc cgcccgacga cctggagccg gcggcgctgg agtagccggt tggcgcggga
     4741 gtcaggtgcg tgaacgaaat ccgtcagatc cgaccgttcg gacggcctat cccgatactg
     4801 tgcgggacag tcggccgcga tccggcgcgg cgccgatccc ggcccggttg attttcatcc
     4861 catcggcggt ccatcatggc gcaagggcag ttgcaccagc cgccgcgcgg cggggcccga
     4921 ccgatgtgcc cagggagccg aaaaatatgg aaaccactcg gatcgcgacg ctggaaaatg
     4981 aggtcgaggc ggcgctgttg ggcgatatcc tgcgcgaacg cgaaattccc catttcattc
     5041 agtcattccg cgacgccgcc tatgacggga tctttcagac gcagaagggg tggggctgcg
     5101 tcgtcggccc gccggagcgc gccgacgagg tccgcgagat tctcgaagat ctgcgcaagc
     5161 aaccgggttg agcgcccgcc cccgctccgc ggcctccgcc gtcaaggaat tccagccctt
     5221 cggcggcgtt agtgcgcgct ggcccgggcg ccgccgtagc gttccagcag gtagtccttg
     5281 agcaattcgt aacgcggcgg cagcgcgtcg taatcctcgg gcaggccctc cagccgggcg
     5341 agcgcgccgg ggacttccgg cgtgaagccc aggagccgct cgatttcgtc ggggaatttt
     5401 gccggatgcg ccgtttcgac caccacggcc ggcgcgccgt ccagcccttc cgcgcggcga
     5461 tactccagca ggccctgcca gccgaccgcg ccgtgcggct ccagcagcag ccggtggtcg
     5521 cgccacgcct ggcggatcgc ggcgcgcgtg gcgtcgtcgg aaacggcgac ggagaacagg
     5581 tcgcggcgca tcgccgcgaa atccggcgcg cggtgcaggc agccggtctc gtcgagccag
     5641 ccgccgtaaa gttccaccag ccgggccaga ttgctcggat ggccgacgtt catcgcgttg
     5701 gaaatgcagg ctcgcgacgg ctcgattcgc gcataccggc ccgtccggag aaagacgggg
     5761 aactcatcgt tcgcgttgac cggcaccacc agtttcttga gtttgagccc catccggccg
     5821 gcgagcatgc agcccatcat gttgccgaag ttccccgagg ggacggccac gaccaccggt
     5881 tcatcgggct cggccacctg cgccgccgca taaaaataat agaccgtctg gggcaacaga
     5941 cggccgatgt tgatggaatt ggccgaggag aggtccaggt cggccaggtc ggggtcgctg
     6001 aaggcccgtt tgacgagcgc ctggcaatcg tcgaatttcc cctccagcgc gatcgtgcgg
     6061 atgttgtcgc ccagcgtggt catctggcgg cgctggcgct cgctgacctc ctcgcgcggg
     6121 aaaagcacca ggacgcgcac gcccggcacg ttgtggaatg cgctggccac cgccgagccg
     6181 gtgtcgccgc tggtcgccgt caggatcagc agttcgcggc gatcttcctg gaggaagcga
     6241 ccgatcagac gcgccatcat ccgggcggcg aagtccttga acgacgcggt ggggccctgg
     6301 tccagccgca gcacgtgcac acgttcaacg cccgcgaccg gctccagcgg cacggcatag
     6361 tcgtaggcgt cgcgacacag cgctcgcagc gcctccggct cgatcacgcc gtcggtgtag
     6421 gccgacagga catcgaaggc gacgtcggca tacggcttgc cgcgcaggtc ggcgatccgg
     6481 tcggccgaca gctccgggat gcggtcgggc aggtagagcc cgcgatcggg ggcctgcccc
     6541 gccagcagcg cctgcttgag atcgacgcgc ggggcctggc cgttggtgct gtgaaaagtg
     6601 aggggagcca tgaggttcgt cttggtggga taggggacgc gccctcccta cgataccggc
     6661 atcacgcgcc cgctggcaag gcggccgatg cgtgctgtca aagcacaata gaaatgtcac
     6721 ccctttgagc acaatagaag tgtcacctg
//
LOCUS       DAOPCO010000218         6742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10075_length_6742_cov_5.362644, whole genome shotgun sequence.
ACCESSION   DAOPCO010000218 DAOPCO010000000
VERSION     DAOPCO010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6742
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_6742_cov_5.362644"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLS90_13160"
     CDS             <1..288
                     /locus_tag="PLS90_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03394.1"
                     /translation="VLGGTAELRGAAEPGRAAEPGGAAEKIKPKMPRRGWALTKEQIN
                     FIRHARDTRTTAQTIEDFYKVFGFKPSERTIRKFRNSDPAKLRLCRPTPFL"
     gene            complement(449..524)
                     /locus_tag="PLS90_13165"
     tRNA            complement(449..524)
                     /locus_tag="PLS90_13165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Thr,seq:ggt)
     gene            complement(786..1097)
                     /locus_tag="PLS90_13170"
     CDS             complement(786..1097)
                     /locus_tag="PLS90_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03395.1"
                     /translation="MDRTVKILLTIIAVLLAILVVRPALRFAPEVTAQTSDRRNAPNP
                     NPEPIIRWHTDLLSTIPHLVNKRKPEIHVINGDSAFLLQYDDRVEVWRIQQRRVVPVS
                     R"
     gene            complement(1284..2405)
                     /locus_tag="PLS90_13175"
     CDS             complement(1284..2405)
                     /locus_tag="PLS90_13175"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK03396.1"
                     /translation="MIRKLILCAAVLTGLLAGPRAGAEPAEQTADHPTTGGAEMPPQA
                     LHPTVVMETTLGDITLQLDAEKAPVSTLNFVNYALNDFYESTIFHRVIPDFMIQGGGY
                     LPDLSEKGGSQGPIVNEWQNGLKNERGTIAMARLGRQPNSATNQFFINTVDNPFLDQP
                     NDGAGYAVFGRVIEGMDVVDKIRNVKTEPSMVSPVTPVEPVVIKEVRFVDEAARAYWA
                     EKTASYDAAMQEAVASHRRVVAAAEARKGQELQEVIAKLEAETGNKVQTTPSGLKYVV
                     WRDGEGESPGPTDRVKVHYEGRFLDGKVFDSSYQRNQPTEFGLNQVIRGWTEGVGMMK
                     PGAKWTLIIPGDLAYGPAGRGEIPPNATLVFDIELLEVK"
     gene            2615..3814
                     /locus_tag="PLS90_13180"
     CDS             2615..3814
                     /locus_tag="PLS90_13180"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPK03397.1"
                     /translation="MLKMIHPDQALDLVLAHAAPLPAETVPLDDAIGRVAAEPIAADR
                     DYPPFHRAMMDGVAVYLSDAGQWVQLVGEVAAGGQWRAALAPGTAVEIMTGAPAPAGA
                     EAVVPREDLTREGDRVRLPDELRGQQNIAPRASAVAAGQPIIQPGDPLSALAVGVLAS
                     VGRDAVRAVRRPRLRIISTGSELVDVAREPGAAQIRDSNAPMLAAFARALGLTGVATG
                     RAADTPDALRDALDQARDAELLVLIGGVSAGRYDLVPQALAEAGVELIFHKVTQKPGK
                     PLLFGRRQGQLVFGLPGNPLSCHFCFHRYVAPAARALAGRRASPPGGAARLTHPWRIK
                     GSRIVFQLGRVEPGADGHELTLLRNADSADLFNTHRANCIARLKPAPGQWEPGARIDF
                     EWLAGEV"
     gene            complement(3928..4293)
                     /locus_tag="PLS90_13185"
     CDS             complement(3928..4293)
                     /locus_tag="PLS90_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HPK03398.1"
                     /translation="MAYVDGFVLTVPKDRLADYVKIARKASKVWMDHGALEVRECVGD
                     DLDIKEMVPFPKLARAKPDETVIFSWIVFKSKADRNRVNAKVMKDPRIEKMMKGSPPF
                     DCKRMGYGGFKMVVNEVAE"
     gene            complement(4364..4795)
                     /locus_tag="PLS90_13190"
     CDS             complement(4364..4795)
                     /locus_tag="PLS90_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HPK03399.1"
                     /translation="MRKLKWLLAPAQHPLYADAALLLIRIVAGLAFMFHGFGKIKNPF
                     GWMGPDGFAPGIFQALAAISEFGGGLAWILGLLMPLGSLGIFCTMSVAFSMHAFVRRD
                     PFVGGPGGSYELALVYLCLAVLFIALGPGRVSLDRLIFGRK"
     gene            complement(4892..5338)
                     /locus_tag="PLS90_13195"
     CDS             complement(4892..5338)
                     /locus_tag="PLS90_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HPK03400.1"
                     /translation="MGQSTVEREIVSSRLIDAPRERVFGAFSDPERLARWWGPAGFTN
                     TFHQFEFRPGGRWRFTMHGPGGTDYENESVFQVIEAPELIVLRHLEPMHAFEMTMTHA
                     AEGGRTRLTWRMVFDTAEECAAVRAYIPRANGENFDRLEAELRRAE"
     gene            5786..6091
                     /gene="rplU"
                     /locus_tag="PLS90_13200"
     CDS             5786..6091
                     /gene="rplU"
                     /locus_tag="PLS90_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPK03401.1"
                     /translation="MSYAIIKTGGKQYRVRQGDRLRVEKLAGDVGAEISLDSVLALGG
                     GADLKLGDGLGLVTAKILAHGRGRKIRIWKSKRRKGYEKRQGHRQSYTEIQITALPA"
     gene            6147..6401
                     /gene="rpmA"
                     /locus_tag="PLS90_13205"
     CDS             6147..6401
                     /gene="rpmA"
                     /locus_tag="PLS90_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPK03402.1"
                     /translation="MAHKKGGGSTKNGRDSNPQFRGCKAFAGQQVTGGSILVRQCGNK
                     FWPGANVGQGRDFTLFAKIPGTVQYAARNNRRYISIVSEG"
BASE COUNT         1128 a         2559 c         1890 g         1165 t
ORIGIN      
        1 gtgctgggag ggacagccga gctaagaggg gcagctgagc caggaagggc agccgagcca
       61 ggaggggcag ccgagaaaat caaacccaag atgccgaggc gcggttgggc attaaccaaa
      121 gagcagatta atttcatccg ccacgcacgc gatacgagga ctacggccca gaccattgaa
      181 gatttttaca aagtgttcgg attcaagccc agcgaaagaa cgatacggaa atttcggaac
      241 tccgacccgg caaagctgag actttgccgc cccacgccgt tcctgtaaga aatgccgcct
      301 tccggcgaaa tgttgcgtcc gtgcttgcgt ccacttggcg tccattttcg ccgtttcttc
      361 gccctatttt ggacaaaaac aaaacccgcc gagtttggcg ggtccggctt aaaaagcctt
      421 atttttcagt gttttttggt gttttcagtg gagcccaaga ccagaatcga actggtgacc
      481 tcatgcttac catgcatgtg ctctgccgac tgagctactt gggctctctc tcgtgacagg
      541 cgtcccagta ctaacccacg ccggggcccg aatgcaaccc ttgagttccc cccattcctc
      601 cggcgcgttc ccccgttcgc ccgcccctgg atgcgtccac cgttcggccc gccccggacc
      661 accgaaccgg tcggcgttcc gccgctgcct gctcgcctga tctcccgatc gctccgagcg
      721 tctccgctcg cccccgccgt cccggatgcc cccgcgcccc ttctttcctc aaatccccgc
      781 cgccgctacc ggctgaccgg cacgacgcgc cgctgctgga tccgccacac ctccacccgg
      841 tcgtcatact gcagcaggaa cgcgctgtcc ccattgatga cgtggatctc cggcttgcgc
      901 ttgttgacca gatgcggaat cgtcgacagt aggtcggtat gccagcggat gatcggctcg
      961 gggttgggat tgggggcgtt gcggcggtcg gaggtctgcg cggtgacctc cggcgcgaac
     1021 cgcagcgccg ggcgcacgac cagaatcgcc agcagcacgg cgatgatcgt gagcagaatt
     1081 ttcacggttc ggtccatctt acgttcgctc cgtggccgga tgggcatgcc gattcgccgg
     1141 ggccgccgcg cgccggacgc caagggacca tgatgatttc cccggcgcaa atgtcaaccg
     1201 gggaactcgg cccgcccccg ccgaagcccc gccggcatgt cggatggacc gtttgaatcg
     1261 gcgcggatct tctcgtgttc tgctcacttc acctcaagca gctcaatatc gaacaccagc
     1321 gtcgcgttgg gcggaatttc cccccgcccc gccgggccat acgccaggtc gcccgggatg
     1381 atcagcgtcc acttcgcccc cggcttcatc atccccaccc cctcggtcca gccgcggatc
     1441 acctgattca acccgaactc ggtcggctgg ttgcgctgat acgaactgtc gaagaccttc
     1501 ccatccagga agcgtccctc gtagtgcacc ttgacccggt cggtcggccc gggcgattcc
     1561 ccctcgccgt cgcgccagac gacatacttc agccccgacg gcgtcgtctg caccttgttg
     1621 ccggtctcgg cctccagctt ggcgatcacc tcctgcagct cctgcccctt gcgcgcttcg
     1681 gccgccgcca ccacccggcg gtggctcgcc acggcttcct gcatcgcggc gtcgtagctg
     1741 gccgtcttct cggcccagta cgcccgcgcc gcttcatcca caaaccgcac ttccttgatc
     1801 accaccggct cgaccggcgt caccgggctc accatcgacg gctcggtctt cacgttgcgg
     1861 atcttgtcca ccacgtccat cccctcgatc acccgcccga agacggcata ccccgcccca
     1921 tcgttgggct gatccagaaa cgggttatcc accgtgttga tgaagaactg gttcgtcgcc
     1981 gagttgggct ggcggcccag ccgcgccatc gcgatcgtcc cgcgttcgtt cttcaggccg
     2041 ttctgccact cgttcacgat cggcccctgg ctccccccct tctcgctcag atcgggcagg
     2101 tagccgcccc cctgaatcat gaagtcgggg atcacccggt gaaagatcgt gctctcgtag
     2161 aagtcgttca gggcataatt gacgaagttc agcgtggaga ccggcgcctt ttcggcatcg
     2221 agctgcagcg tgatgtcgcc gagcgtggtt tccatgacga cggtcggatg aagcgcttga
     2281 gggggcattt cggcaccgcc ggtggtagga tggtcggccg tctgctcggc cggctcggcg
     2341 ccggcgcgcg ggccggccag cagccccgtc aggaccgccg cgcagaggat cagcttgcgt
     2401 atcatgggcc agtctcctgt ggacaatcgg tgttgggaca tgacgggaag cgccgcgcat
     2461 ggcgcgcgga ctggccacca gtctaggcca ttccgccccg actgacaaga tcgaccggac
     2521 gaatgttcct tacatccctt gcgtccctta cgtccctgat gtccctgatg tcaccctcgt
     2581 cctttccccg atcgccaacg acggagtcaa cgccatgctc aaaatgatcc accccgacca
     2641 ggccctcgac ctggtgctgg cccacgccgc gccgttgccc gccgaaacgg ttccgctcga
     2701 cgacgccatc ggccgcgtcg ccgccgaacc gatcgccgcc gaccgcgact accccccctt
     2761 ccaccgcgcg atgatggacg gcgtcgccgt ttacctcagc gacgccggcc agtgggtcca
     2821 gctggtgggc gaggtggcgg ctggcggcca gtggcgcgcc gcgcttgccc ccggcacggc
     2881 tgtcgaaatc atgaccggcg cgcccgcccc cgccggcgcc gaggcggtcg tcccgcgcga
     2941 ggacctgacc cgcgagggcg accgcgtccg cctgcccgac gaactgcgcg ggcagcagaa
     3001 catcgccccc cgcgccagcg ccgtcgccgc cggccagccc atcatccagc cgggcgaccc
     3061 cctcagcgcg ctggccgtcg gcgtcctggc gtccgtcgga cgcgatgccg tccgcgcggt
     3121 gcgccgcccc cgcctgcgca tcatctcgac cggcagcgaa ctggtcgacg tcgcgcgcga
     3181 gcccggcgcc gcgcagatcc gcgacagcaa tgccccgatg ctggccgcgt tcgcccgcgc
     3241 gctcggcctc accggcgtcg ccaccggccg cgcggccgac acccccgacg cgctgcgcga
     3301 cgcgctcgac caggcgcgcg acgccgagct gctcgtcctc atcggcggcg tctccgccgg
     3361 ccgctacgat ctggtccccc aggccctcgc cgaggccggc gtcgagctga tctttcacaa
     3421 agtaactcag aaaccgggca agccgctcct cttcggccgg cgccagggcc agctcgtctt
     3481 cggcctgccc ggcaaccccc tctcctgcca tttctgcttt caccgctacg tcgcgccggc
     3541 cgcgcgcgcg ctggcgggcc gccgcgcctc ccctcccggc ggcgccgccc gcctcacgca
     3601 tccctggcgg atcaagggct cgcgcatcgt ctttcaactg ggccgcgtcg agccgggcgc
     3661 cgacggccac gagttgaccc tcctgcgcaa cgccgactcg gccgacctct tcaacacgca
     3721 tcgcgccaac tgcatcgccc ggttgaagcc cgcgccggga caatgggaac ccggcgcccg
     3781 gatcgacttc gagtggctcg cgggcgaagt ctgaccccgc ccgaccgcac aacgcgcccg
     3841 ggaacgaagg ccggcaaaat ccgtccgatc ggtcggttcc gtccgatccc acggatttct
     3901 gattccagcc gcccccgcgc cgacgcccta ctcggcgacc tcattgacga ccatcttgaa
     3961 cccgccgtag cccatccgtt tgcaatcgaa cggcggcgac cccttcatca ttttctcaat
     4021 ccgcgggtct ttcatcacct tggcgttgac ccggttgcgg tccgccttcg acttgaacac
     4081 gatccacgag aaaatgaccg tctcgtcggg cttggccctc gccagcttgg ggaagggcac
     4141 catctccttg atgtccagat cgtccccgac gcactcgcgc acttccagcg ccccgtggtc
     4201 catccagacc ttgctcgctt tccgcgcgat cttcacgtag tcggccagcc tgtccttcgg
     4261 gacagtcagc acgaacccat cgacgtaggc catcaggcgg ctccttccat cacggcagag
     4321 tatgtccggc ggcgcgataa cggcgccccc ggcggaccga acgctacttc cggccgaaaa
     4381 tcaaccgatc cagcgacacc cgccccggcc ccagcgcgat gaacagcacc gccaggcaca
     4441 ggtaaaccag cgccagctca taggagcccc ccgggccccc gacgaacggg tcgcggcgca
     4501 cgaaggcgtg catcgagaac gccacgctca ttgtgcagaa gatccccagc gaaccgagcg
     4561 gcatcagcag ccccagaatc catgccaggc cgccgccaaa ttccgaaatc gccgccagcg
     4621 cctggaagat ccccggcgcg aacccgtccg gtcccatcca cccgaacgga ttcttgattt
     4681 tgccgaatcc gtggaacatg aacgccaggc cggcgacaat ccggatcagc agcaacgccg
     4741 cgtccgcgta aagcggatgc tgcgccgggg ccaacagcca tttcagtttc ctcatctttt
     4801 ccgccttcct atcagttggg tttcccggtc tggaccggaa acgtcttcga cccatccccg
     4861 cgctcatcct tggcgcaacg cgcgccccgc tctactccgc tcgacgcagc tcggcctcca
     4921 gccggtcgaa gttctcgccg ttggcccgcg gaatataggc gcgcacggcc gcgcattcct
     4981 ccgccgtgtc gaacaccatc cgccacgtca gccgcgtccg gcccccctcg gcggcgtgcg
     5041 tcatcgtcat ctcgaaggca tgcatcggct ccaggtggcg caagacgatc aactccggcg
     5101 cctcgatcac ctgaaacacg ctctcatttt cataatcggt tccacccggt ccgtgcatcg
     5161 taaaacgcca gcggcccccc ggccggaact caaactgatg aaacgtattg gtgaatccgg
     5221 ccggccccca ccagcgcgcc aggcgctccg gatcgctgaa cgcgccgaac acccgctcgc
     5281 gcggcgcatc gatcagccgg ctcgaaacga tctcccgctc caccgtgctc tgtcccatcg
     5341 cccatcctcc ccaaaattcg tccctcgact tcggctctcg aattctttcc cgcgcgagcg
     5401 acgcccggca actaccccga ccaggcgccc gaattatatg atgaaaacgt caacaattgg
     5461 gagcacgcat ttcttccgac acccactcca ctcgatcaca agatccgcta atcttccccc
     5521 atcccgtctc tcgcatcctc cccgttttcc ccgtcccatg cccacaaacc cgcccccgag
     5581 catccttgga gaaacaggac attcttgctt tgcagaaaat gaggacatac ttgcattgcg
     5641 gtgacaacct gcctttttcc ccttgcggcc cgcgccccgg aacgctataa gttttgtttt
     5701 gcgcccaggc ggcgcgaacc ggggatctgt gtctccaccg acccgcgggg ggattcggat
     5761 cgctcgccaa ggagttgaac gcaccatgtc gtacgccatt atcaagaccg gcggcaaaca
     5821 ataccgcgtc cgccagggcg accgcctgcg cgtcgaaaag ctcgccggcg acgtcggcgc
     5881 cgaaatcagc ctcgacagcg tcctggccct gggcggcggc gccgacctca agctgggcga
     5941 cggcctcggc ctcgtcacgg ctaaaatcct cgcccacggc cgcggccgca agatccgcat
     6001 ctggaagtcc aagcgccgca agggctacga aaagcgccag ggacaccgcc agtcctacac
     6061 cgaaattcag atcacggctt tgccggcctg accgcacggc ccgcaccgga cctgaccccg
     6121 aaccacgcta acggagagag atcgtcatgg cacacaagaa aggcggcggc tcgaccaaga
     6181 acggccgcga cagcaacccc caattccgcg gctgcaaggc cttcgccggc cagcaggtca
     6241 ccggcggctc gatcctcgtc cgccagtgcg gcaacaagtt ctggccgggc gccaatgtcg
     6301 gccagggccg cgacttcacc ctcttcgcca agatccccgg caccgtccag tacgccgcgc
     6361 gcaacaaccg gcgctacatc agcatcgtca gcgaaggctg atccggcgcc aagccggtaa
     6421 cgattgcctc aaggttcttc acggcggcgg ccgcgcgaca ttgcgcccgc cgccgttccc
     6481 tttagattcg tcctcggcct cggcctcgta atcgcccctg tcggccggcc cgaaaattcg
     6541 gctctcggct ctcgtccctc gtcctcggct ctcgaaagcc gcccatctca tcccttcgcc
     6601 gttcggcatc gcatcgctat cgaattcggg atcggagtgg ctcctgagcc cggggttcgc
     6661 cccccgcgtc cgcctccgcc cttcccatct cccgtgcccc ctctcccatt tcccttgcca
     6721 ttcgcccgcg aaatggggat ct
//
LOCUS       DAOPCO010000219         6709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10140_length_6709_cov_5.125939, whole genome shotgun sequence.
ACCESSION   DAOPCO010000219 DAOPCO010000000
VERSION     DAOPCO010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10140_length_6709_cov_5.125939"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..1680
                     /locus_tag="PLS90_13210"
     CDS             385..1680
                     /locus_tag="PLS90_13210"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPK03403.1"
                     /translation="MSDSKTARLEIEGKTYEFPTIEGTEQEKAIDIAKLRSSTGYITF
                     DSGYGNTGSCLSSITFIDGEKGILRYRGIPIEQLAENSTFIETAYLIINGELPKRSEL
                     ARFSQGLTDNEMIHEGLKHNFEGFPPTAHPMAILSSMINAMSCYHPQLLEIECDMHFE
                     AAVTRLLSKVRTIAAFSYKKSKGHPFIYPRPDLSYCQNLLHMMFSIPYKDYENDPAVV
                     DALNLLLLLHADHEQNCSTSTVRMVGSSGANLFASVAAGVCALWGPLHGGANMAVIEM
                     LDQIMASGMSVKEYIAKVKDKQSHIRLMGFGHRVYKNFDPRAAIIKRACDRVLDKLGI
                     SDPALDVAKELEEAALHDSYFVERKLYPNVDFYSGIIMRAIGIPLNMFTVMFAIGRMP
                     GWIANWREVHKDPESRIYRPRQIYTGPTERPYVKMEDRG"
     gene            1980..3581
                     /gene="nadB"
                     /locus_tag="PLS90_13215"
     CDS             1980..3581
                     /gene="nadB"
                     /locus_tag="PLS90_13215"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPK03404.1"
                     /translation="MQIEVDVLIIGAGIAGAAAALTLADAGAEVLLLHRSADPNQTNT
                     RWAQGGIIYTSPNDSPELLARDIEQAGAGLTNPPAARLLAEEGPRLVKQFLMDRAGVP
                     FDRGPEGELDITEEGAHSVPRIIHCQDRTGWGIQQALYETIAREPRITVKRGWTAIDL
                     LTLSHHSREPLDRYEPHTCVGAYVLDRDAGQVVTVLAHAVVLATGGLGGLFLHTTNPP
                     GARGDGIAMAYRAGARLTNLEFIQFHPTALYKPPAPRFLISESMRGEGARLITRDGEE
                     FMRRFHPQGSLAPRDVVARSIHEVLLASGEPCVYLDLTYKDSEWIKRRFPTIYHECLR
                     LGIDITQEPIPVVPAAHYSCGGVHTDLWGRTTINRLWAVGEVACTGLHGANRLASTSL
                     LEGLVMGHRAAEDIAAGLAERRRAPWPVIDEWVDETEPSDPALILQDWLTIKYTMWNY
                     VGLARTQKRLKRAYSLLRELQQEVEEFYARTRIDDDLIGLRNGVQAALAVLYAAMQNH
                     VSRGCHYLDDAPESRNRGAAQAITR"
     gene            3591..5135
                     /locus_tag="PLS90_13220"
     CDS             3591..5135
                     /locus_tag="PLS90_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03405.1"
                     /translation="MSRWLAPSLWLIVGLICAVAWPPEPAAQIGQLDARRDNLRATRG
                     GVGSRISRDSRFDRSDRQDRRPSSSRRTRRGEDPSRAGRRDRSRDRDRDGGREAQRDV
                     QAAAGPAEGPARARGGQERSEVARPPRGGGRRAAAGGGTGAPLPQLQINPHLRDDALY
                     FTPTRAYPRVGDRFISTINFFKNDKGPVEALDLWIKYDPHLLEPTWVDLSPAGAGAEA
                     GLEARVWREEGRLRIAGQLPAPLVEQIEELARVHWRALGPAVQTLIEFEAPAGATTAV
                     YSGGRNLVQPSNLGNLSRLSMAIRIGTEETPEPGLRLMREVRETLETASLPREENRVR
                     LALVAPKTPLGEPELVATGEIGLLDVVLLNPDKELIDEVRFRLRYDPNAVKLLDADED
                     NYITQGINIFDGDFHEEFPFDFHGRNEVDPHLGAITYHVGSQTAPLAYPSGTIARIVY
                     RMEREAGQALFWFEREEPETGRLLTDVSRAGISLLGAEPAVARAALHGTAVTVEPLVG
                     APAPMP"
     gene            complement(5143..6267)
                     /locus_tag="PLS90_13225"
     CDS             complement(5143..6267)
                     /locus_tag="PLS90_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK03406.1"
                     /translation="MRYRYTQFDERFAQEQREEQLTKLFLELLMHSGGDVDEALNWLQ
                     DLADRHGLWPEGMDLESFKELLADKGYIQSERSPGKGRLGEPSRERFKPTRKAEKAIR
                     TDAFEELFARMRQDTMGGDHVTPFAGAGGDRLPETRPFGFGDRVQDIDCLGSLTNALR
                     RTGSDSPDMSEDDLQVYELEQATSSATVLALDISHSMILYGEDRITPAKKVALALTEL
                     IQTRYKKDALDVILFGDEAFEVDPRKLPYVQVGPYHTNTKAALELAQQILARKKHANK
                     QIVMITDGKPSAIYVGKKLYINSFGLDARIVNKTVEEAQACRRKGIIITTFMITSDPY
                     LKQFVERLTEANKGRAFYADLDNLGKFVLTDFVRNRRKKV"
BASE COUNT         1180 a         1947 c         2443 g         1139 t
ORIGIN      
        1 cgtgtttgct ccgcgatgga ggggcatcgg tcaatttgcc gtgaaggatc aagggggggg
       61 caagttgcgg accgggtgaa cttgacgaag ggagaggcgc aaattagaat gcgggggtcg
      121 aactgggtgc cagccgatcc aagggatgga atcagcaagt ggcgcgccgc gtgggcgtcg
      181 cggggttccc ggttcgtcag gcaagtccgc cttacaccga gaaggatggc agctccgccc
      241 gcagcgggcg ggcgtcggcc agcgcgcgcg gcgcgccggc gaccaacgga gatgactgcc
      301 cggcgagcga acggggcgcc cccgggtccc cgcggccgga gactcggcat cccgtccagc
      361 aatcccccaa ttgaggtttc acccatgtcc gacagtaaga ccgcccggct cgaaatcgag
      421 ggcaagacgt atgagttccc gacgatcgag gggacggaac aggagaaggc gatcgacatc
      481 gccaagctgc gcagctcgac cgggtatatc acgttcgaca gcggctacgg caataccggc
      541 tcatgcctga gttcgatcac gttcatcgat ggtgagaaag ggatactgcg ttaccgcggg
      601 atcccgatcg agcaactggc cgaaaattcc acgttcatcg aaacggcgta tttgatcatc
      661 aacggtgaac tgcccaaacg cagcgagctg gctcggttca gccagggcct gaccgacaac
      721 gagatgatcc acgaggggct gaagcacaat tttgaggggt ttccgccgac ggcgcatccg
      781 atggcgatcc tctcgtcgat gatcaatgcg atgagttgct accatccgca actgctggaa
      841 atcgaatgcg acatgcattt cgaagcggcc gtgacgcgac tgctgagcaa agtgcggacg
      901 atcgccgcgt tctcgtataa gaagtcgaag ggccacccgt tcatctaccc gcggcccgac
      961 ctgtcgtatt gccagaatct gctgcacatg atgttttcga ttccgtacaa ggactacgag
     1021 aatgatccgg cagtggtgga tgccttgaat ctgctgctgc tgctgcacgc cgatcacgag
     1081 cagaactgct cgacgtcgac cgtgcggatg gtcggttcga gcggggcgaa tctgttcgcc
     1141 tcggtggcgg ccggcgtgtg cgcgctgtgg ggtccgctcc acggcggcgc caacatggcg
     1201 gtgatcgaga tgctggacca gatcatggca tcggggatgt cggtcaagga atacatcgcc
     1261 aaggtgaagg acaagcagag ccacatccgg ctgatgggat tcggccatcg ggtttacaag
     1321 aactttgatc cgcgggcggc gatcatcaag cgcgcctgcg accgggtgct ggacaagctg
     1381 gggatttcgg atccggcgct ggatgtggcg aaggagctgg aagaggcggc gctgcacgac
     1441 agctatttcg tcgagcgcaa gctgtatccc aacgtcgatt tctacagcgg gatcatcatg
     1501 cgggcgatcg ggattccgct caacatgttc acggtgatgt tcgctatcgg gcggatgccg
     1561 ggctggatcg ccaactggcg cgaagtgcac aaggatccgg agagccggat ctaccggccg
     1621 cggcagatct acaccggacc gacggaacga ccgtatgtca agatggagga ccgcggctga
     1681 gggatttttg atccagccga tcaggtcgac agcgcatgga tgaggaagag ggcggccacc
     1741 aggccgccct cttttcacag gtgggacggg gcgcgaagga ggttgggggg gaggacggaa
     1801 gccggcgcta tacaacccga tgccaagttt ctaagatgac agcgagggcg ggtttcatct
     1861 gtcacgaggg tgcgaggacc aggagtgaca ggaattgcgg cgaaagggcg gtcgggggga
     1921 atctcgacag ccgaggacga agacgaggga cgattttgga gtgaccgggc ggatgcggca
     1981 tgcagattga agtggatgtg ctgattatcg gcgcggggat cgcgggcgcg gcggcggcgt
     2041 tgacgctggc cgacgccggg gcggaggtgc tcctgctgca ccgcagtgcc gacccgaatc
     2101 agacgaacac gcgctgggcg cagggcggca tcatttacac cagcccgaac gacagcccgg
     2161 agctgctggc gcgcgatatt gagcaggccg gggccggcct gaccaatccg ccggcggcgc
     2221 ggcttctggc ggaggaaggc ccgcgactgg tcaagcagtt cctgatggac cgggccggcg
     2281 tcccgtttga ccgggggccg gaaggggagc tggacatcac cgaggaaggg gcgcactcgg
     2341 tcccgcggat aatccactgc caggatcgaa ccggctgggg gattcagcag gcgctgtacg
     2401 agacgatcgc gcgcgagccg cgcatcacgg tcaagcgggg ctggacggcg atcgacctgc
     2461 tgacgctgtc gcaccactcg cgcgagccgc tggaccgtta cgagccgcac acgtgcgtgg
     2521 gggcctacgt gctggaccgc gacgcggggc aggtggtgac ggtcctggcg catgcggtgg
     2581 tgctggcgac agggggtctt ggtgggctgt tcctgcacac gaccaacccg ccgggggcgc
     2641 gcggcgacgg gatcgcgatg gcgtatcgcg cgggggcgcg gctaaccaac ctggagttca
     2701 ttcaattcca cccgacggcg ctctacaagc cgccggcgcc gcgatttctg atttcggagt
     2761 cgatgcgggg cgagggagcg cggctgatca cgcgcgacgg cgaggagttc atgcggcgat
     2821 ttcacccgca gggctcgctg gcgccgcgcg acgtggtggc gcgctcgatc cacgaggtgc
     2881 tgctggccag cggcgagccg tgcgtctacc tggatttgac ctacaaggat tcggagtgga
     2941 tcaaacggcg gtttccaacg atctaccatg aatgcctgcg cctggggatc gatatcacgc
     3001 aggagccgat cccggtcgtg ccggcggcgc actacagttg cggcggcgtc cataccgacc
     3061 tgtgggggag gacgacgatc aaccggctgt gggcggtggg cgaggtcgcg tgcacggggt
     3121 tgcacggggc gaaccggctg gcgtcgactt cgctgctgga ggggctggtg atgggtcacc
     3181 gcgccgccga ggacattgcg gcggggctgg ccgagaggcg gcgcgcgccg tggccggtga
     3241 tcgacgaatg ggtcgatgag acggagccgt cggacccggc actgattttg caggactggt
     3301 tgacgatcaa gtatacgatg tggaactacg tcggacttgc tcgcactcag aaacgcctca
     3361 agcgcgccta ctcgctgctg cgcgaactgc agcaggaagt cgaggagttt tacgcgcgga
     3421 cgcggatcga cgatgacctg atcggcctgc gcaacggggt gcaggcggcg ctggccgtgc
     3481 tctacgcggc gatgcagaac catgtcagcc gcggctgcca ctacctggac gacgcgccgg
     3541 agtcgcgaaa ccgcggcgcc gctcaggcga ttacccgata ggagcgaccg ttgtcgcgct
     3601 ggcttgcacc gtctctgtgg ctgatcgtgg ggctgatctg cgcggtagcg tggccgccgg
     3661 aaccggcggc gcagatcggc cagctcgatg cgcggcgcga caatctgcgc gccacgcgcg
     3721 ggggcgtcgg cagccgcatt tcgcgcgaca gtcggttcga ccgatcggat cggcaggacc
     3781 gccgccccag cagcagccgg cggacgcgga ggggcgagga cccgagccgg gcgggtcgcc
     3841 gcgaccggtc gcgcgatcgt gatcgcgacg gcgggcgcga agcccagcgc gatgtgcaag
     3901 ccgccgccgg tccggccgag ggcccggccc gcgcgcgcgg cggtcaggaa cggagtgaag
     3961 tggcgcgccc gccccgcggc ggcggccggc gggcggcggc gggcggcgga accggggcgc
     4021 cgctgcccca gcttcaaatc aacccccacc tgcgcgacga cgcgctctac ttcacgccga
     4081 cgcgggccta cccgcgggtg ggcgaccggt tcatttcgac gatcaatttc ttcaagaacg
     4141 acaaggggcc ggtcgaggcg ctggacctct ggatcaagta tgatccgcat ctgctggaac
     4201 cgacctgggt ggacctgagc ccggccggcg cgggcgcgga agcggggctc gaagcgcggg
     4261 tctggcgcga agagggccgg ctgcggattg cgggacaact gccggcgccg ctggtcgaac
     4321 agatcgagga gctggcgcgg gtccactggc gggcgctggg gccggccgtg cagacgctga
     4381 tcgagttcga ggcgccggcg ggggcgacga cggccgtcta ttccggcggc cggaatctgg
     4441 tgcaaccgtc caacctgggc aatctgtcgc ggctgagcat ggcgattcgg atcggcacgg
     4501 aagagacgcc cgagccgggc ctgcgcctga tgcgcgaagt gcgcgagacg ctggagacgg
     4561 ccagcctgcc gcgcgaggag aaccgcgtcc ggctggcgct ggtcgcgccg aaaacgccgc
     4621 tgggtgaacc ggagctggtc gcgacgggcg aaatcgggct gctggatgtg gtgctgctca
     4681 atcctgacaa ggagctgatc gacgaagtgc ggttccggct gcgctacgat ccgaacgcgg
     4741 tcaagctgct cgacgccgat gaagacaact acatcaccca gggaatcaac attttcgacg
     4801 gggattttca cgaggaattc ccgttcgatt tccatggccg gaacgaagtg gatccgcatc
     4861 tgggagcgat cacgtatcat gtgggatcgc aaacggcgcc gctggcgtac cccagcggga
     4921 cgatcgcgcg catcgtgtac cggatggagc gcgaggcggg gcaggccctc ttctggttcg
     4981 aacgcgagga gcccgagacc gggcggttgt tgacggacgt ttcgcgcgcg gggatcagcc
     5041 tgctgggggc cgagccggcg gtggcgcggg cggcgctgca cgggacggcg gtgacggtgg
     5101 aaccgctggt cggcgcgccg gcgccgatgc cgtgacgggc gttcagactt tttttctgcg
     5161 attgcggacg aaatcggtca gcacgaattt tcccagattg tcgagatcgg catagaacgc
     5221 gcggcccttg ttcgcctcgg tgagccgttc gacgaactgc ttgaggtagg ggtcggaggt
     5281 gatcatgaac gtggtgatga tgatcccctt gcggcggcag gcctgggcct cctcgaccgt
     5341 cttgttcacg atccgcgcgt cgagtccgaa ggaattgatg taaagcttct tgccgacgta
     5401 gatcgcgctg ggtttgccgt cggtgatcat cacgatctgc ttgttcgcgt gcttcttgcg
     5461 ggcgaggatc tgctgcgcca gctccagcgc cgccttggtg ttggtgtgat acggtcccac
     5521 ctgaacgtag gggagtttgc gcgggtcgac ctcgaatgcc tcgtcgccga agaggatcac
     5581 gtcgagggcg tccttcttgt agcgggtctg aatcagttcg gtgagggcga gggcgacctt
     5641 cttggccggc gtgatgcggt cctcgccgta gaggatcatc gaatgcgaga tgtcgagcgc
     5701 gaggacggtg gcgctgctgg tggcctgttc cagctcgtag acctgcaggt cgtcctcgct
     5761 catatcgggc gagtcggagc cggtgcggcg cagggcgttg gtcaggctgc ccaggcaatc
     5821 gatgtcctgg acgcggtcgc cgaagccgaa ggggcgggtc tcggggaggc ggtcaccgcc
     5881 ggcgccggcg aacggggtga cgtggtcgcc gcccattgta tcctggcgca tgcgggcgaa
     5941 gagttcctcg aaggcgtcgg tgcggatggc cttttcggcc ttgcgggtgg gtttgaagcg
     6001 ttcgcggctg ggctcgccaa ggcggccctt gcccggcgag cgctcgctct ggatgtagcc
     6061 tttgtcggcg aggagttcct tgaacgattc gaggtccatg ccttcgggcc agaggccgtg
     6121 gcggtcggcg agatcctgga gccagttgag cgcctcgtcg acatcgcccc ccgaatgcat
     6181 gagtaattcg aggaagagtt tggtcagttg ctcctcgcgc tgctcctgag cgaagcgttc
     6241 atcgaactgg gtatagcggt agcgcatggt ggatcttcag aattgaaccc ggcaaatgtc
     6301 ggtctgccgg cgcggtccta ttgtaccgct tccagccgtc tcgagatggc accggcgcga
     6361 aggccgttgt ccaatcgagc ttctttgtcg taaccgttac cacggtttgg ccaaattgaa
     6421 ggcatcatga ttggcctcag ctcttatgtc tcggctcgaa cgagcgaagg aagccgttga
     6481 agcggctccc atcatccgcc caacctgaca ccggcctgaa ggccggcgct aatgagacac
     6541 cgcgaaagcg gcgaaaaagg ctgctgcgca ggctgctcgg ttggcggaca gcttcttccg
     6601 gcggcggtgg tccatgtcag tggcaatcat cgaccgtgcc ctaatcatcc gccttcatgg
     6661 cgaagagttc gtcggccgtc agcccgattc gtacggggtc ggggtaggt
//
LOCUS       DAOPCO010000220         6674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10197_length_6674_cov_4.326484, whole genome shotgun sequence.
ACCESSION   DAOPCO010000220 DAOPCO010000000
VERSION     DAOPCO010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_6674_cov_4.326484"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="PLS90_13230"
     CDS             <1..811
                     /locus_tag="PLS90_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK03407.1"
                     /translation="GRAIFHLGADKKVSHGQAGPFAECEDLAEIEAFEWPNPDYLNFG
                     DSLRALEQAGPYYRASGFWTCFHHNVMDLFGIDAYMMKMYTHPEQVHAVTDHVCQFYY
                     EANERFYQAAGPLIDGFFFGNDFGTQADLFIGPDQFDEFIMPWVRRFVEQAHRHGYQV
                     ILHSCGSIFRVIDRLIEAGVDCLHPLQARARQMDAETLARHFNGRISFLGGIDTQDLL
                     VHGSPDEVQREVERISELLGPRYIISPSHEALLPNVSPANVAAMAQALEIF"
     gene            1865..2572
                     /locus_tag="PLS90_13235"
     CDS             1865..2572
                     /locus_tag="PLS90_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03408.1"
                     /translation="MSRVRVAALLAVCLLQFSPAAEQVGLQPGWTEQNRVEVSTTTNF
                     PSWNDPITVSLAVESQGPPLIESGDTYFVAHGQTPVLLQHIYPDVMPRDAEVTQARMG
                     TDHIAQTDAVELSATVSEIGNQVDGFFEAFGSQAGYVWIANQIFVGHALARIEYRLGE
                     GMPVSSFDPWGVYPDLTDLTDSPDILNLTFKVKALASDPLNEEHDNPRVRRPVARTLA
                     TGLSGRHVLPVALVRVR"
     gene            2514..6440
                     /locus_tag="PLS90_13240"
     CDS             2514..6440
                     /locus_tag="PLS90_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03409.1"
                     /translation="MRPAYREDTFYLSPWYESDEKGDVEIQVGGGGAVIEPINDLWVR
                     PPFAVLEADGGAVGYGENRLKIEDLWTFKGTLKIRVKDPAGRPVTGVRVSLEGAVQAF
                     SLYSDSAGEVDVTVNGGGSDTRTVELTVVPRMLLDSSLTQIVDNVQESQLHGNTPVFY
                     VAKFRLDDSINYLTSGDPFDDFTLNIYENDLLIAQIDGLTAKCAAAMTPADHARGVNA
                     YHYPVLPEATPKSYRHQIVWKNALGEVMPLSPLWSPRTIRERRVPALERPFRIHYVGI
                     DTKGAYKAHGRNIMETWASPARMAKINDYFQMLYPGPVEFTYGPDLVASEPWYTFGPY
                     QVLSYFVDLARLRESMPDRPDLIVGVSAAGVIGADGLSEPRFREVILLDGCSMRESYL
                     LHEYLHTLGLLDNYEYETGEGTYGASANGYDPRTMRRIYNPPGVDKPPHQTVMYDRAP
                     SPWPTTEGYSKLLDEATVAFIDNAKDSARAEEKAIEPLAAPPQEVMLIGGVYLGRQSW
                     ADYWYTMRDVWPIFVDTDVAWQPQQTATSNARALGIRLSNGQTASVLEDRYWIWGAYD
                     GQEAGQLAAAPLFKIPYDPATNAVTFQAQYTGDLVRTIREVPFSPHAPTVSIVSPAAG
                     SRLAGHAPLVFQADDVDAGEQLYAWIKLSGDGGATWTPVGNWFPIPQRQNTFALPCDD
                     LPTLDAARLRVLISDGTRSARADAGPFALDGYMPGPRAAADPAWHEWTVRTGSRCDLP
                     VKIVNSGRAALEVSFDAGAKPSWLTCRQLGKQSIVAGGSFEFLFECAAGAAGEQNAEL
                     RFLTNDAQQPVLTVPLKLRLVETATAPVVATVATDPPADAGERTALRNTPVRFVVREA
                     CGRAGLAGRIEIHHAETGEVLAVVDLAPGTVPGLYQAEWDVPAGCFGVPLGVEATLTD
                     SASGLSSSGGSNPVGWDARLQLMRRNTAPRFINPTNDNYVDLIIPQTLEIPFEAVDDE
                     GDPISFAVRHTSDLNVRLDAEARKLYVALLVDAQTPLSQIWLAACDPWGATSGVALTI
                     RVGWQNTNYGHLAYLSNFLLETNPMPLAIAGGWSMEWPQKVVLDYRAEGAPAWTRLGE
                     YEFTEWDNLKNCWIAHCLWDWPTDGTAVFELRVTLVGSNGSADPEPPVYRFERPRQGG
                     RVTRVEAPSPVMAGAEFTLRIHVANDSSDRWSAESGHGLIALDGVDPLTGLATAEFMG
                     LQSIERGSNGVVEVRATAPAAPGVYRTAWGLRVGSTPLGTGAEATVQVVARAADALAA
                     STLIDVLLGRVAMQSLIPGTLDLNGDGQIDVADLVLLLNRSAGGGV"
     gene            complement(6492..>6674)
                     /locus_tag="PLS90_13245"
     CDS             complement(6492..>6674)
                     /locus_tag="PLS90_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HPK03410.1"
                     /translation="GVAPTSVVQTIEPLFVLPMAWWWLGRILAGKELGGGALILGGAI
                     GLVILHARPARTGRRP"
BASE COUNT         1209 a         2127 c         2144 g         1194 t
ORIGIN      
        1 ggggcgcgcg atcttccacc ttggcgcgga caagaaagtc tcgcacggcc aggcggggcc
       61 cttcgccgaa tgcgaggacc tcgcggaaat cgaagccttt gaatggccga atcccgatta
      121 tctgaacttc ggcgattccc tgcgcgcgct ggagcaggcc gggccctatt accgggccag
      181 cggtttttgg acctgcttcc atcacaacgt catggacctg ttcggcatcg acgcctacat
      241 gatgaagatg tacacgcatc cggaacaggt ccatgccgtc acggatcatg tctgccagtt
      301 ttactacgaa gcgaacgagc gtttctacca ggcggccggc ccgctgatcg acggcttttt
      361 cttcggcaac gattttggaa cgcaagcgga tctgttcatc gggccggacc agttcgatga
      421 attcatcatg ccctgggtcc gccgcttcgt cgagcaggcc caccggcacg gttaccaggt
      481 cattctgcat tcgtgcggct cgatttttcg ggtcatcgat cgcctgattg aggcaggcgt
      541 ggactgcctg cacccgttgc aggcccgggc gcgacagatg gacgccgaaa cgctggcgcg
      601 ccatttcaat ggtcgtattt cgtttctggg cggcatcgac acccaggatt tgctcgtgca
      661 tggcagcccc gacgaggtgc agcgcgaggt cgaacgcatc agcgagctgt tggggccgcg
      721 ctacatcatc agcccgagcc atgaggcgtt gttgccgaat gtctcgccgg ccaacgtggc
      781 ggcgatggcc caggccctgg aaattttctg aaccggagcc ggccgctgac ccgggatggg
      841 atgggcgagc gcccggtacg gcgagtagtg atcgccgccg ccggcgcgag acaagcgcgg
      901 caattgcgcc gatggagacg tctcccggcg gcttgcccgc gcgctcgtag catttccagg
      961 aatgcgggct cacggatgcg ttctcctgcg aaaagcaggc gttgtcggtg aaggcccaca
     1021 gcttgtcccg gaagccgagg accgaggagt tgtcggactt gaagggagcc tgttgtagga
     1081 ccggcttcca ctggacgcca tccgtgctgc gcctgccatc gggcaaaaat aagccgcgat
     1141 gcgacttaat gatgcctccc aacccccgat cagccagaac tagccgtcga agacggtaaa
     1201 cgccgcgccc ccgcgaagga tgaacgaggc ttccacgggc agcggctccc agtcggcccc
     1261 gtccgccgag gaccatccgg accctcttac cgcgtcaatc agccagatcc gcccaaatgc
     1321 cgccgttgca cattcgggtg ccagcgcgct gaaggggggc cgagtccgtc gcctgattcc
     1381 aatccaacgc atctggcgct cctcgaagct tctccgggaa gtggattccc gccgccgctt
     1441 gaagcgatcg cgggttgggg ccgaattgcg ggttggcgcc aacgagttcg gctttccggg
     1501 catcccggct gagggatcct ttccggcaga agatggatgc ctcgatctcg tcgcatcgaa
     1561 gcttcaaaaa tggttggtcg tttggatccc ttgctggcgt tatgaacggg atatgcagga
     1621 agcccatgcg ccgtcaagaa tcacaagatg acagcatgcc aacatcctgt ttagatcccg
     1681 atgcagccgg cgctgcgtat ggcggcgtcg gcggagagcg gacccggtcg ggcgacgatt
     1741 ttggctggac acaggggcgg cgggagtggt aggctcaata aagagaattg aagctcccga
     1801 tctatgattt atgtatcaca atagatcatt aagacgaact ttatagtctg taaaaggacg
     1861 aatcatgtcg cgtgttcgtg ttgccgccct gctggcggtt tgtctgctgc aattctcccc
     1921 cgccgccgaa caggtcgggc tgcagcctgg ctggacggaa caaaaccgcg tggaagtcag
     1981 caccaccacg aattttccga gttggaatga cccgatcacg gtcagtttgg ccgtcgagag
     2041 ccaggggccc ccgttgattg agtcgggcga cacttatttt gtcgctcatg ggcaaacgcc
     2101 ggtgctgctg caacacatct atcccgatgt catgccgcgc gacgccgagg tgacacaggc
     2161 ccggatgggg accgatcata ttgcgcaaac cgatgcggtc gaactgagtg ccacggtcag
     2221 tgagatcggc aaccaagttg acggcttttt tgaagctttc gggtcccagg ccggctatgt
     2281 ctggatcgcc aaccaaattt ttgtcgggca cgccctcgca cgcatcgaat accgcctcgg
     2341 ggaaggcatg cccgtgtcca gcttcgatcc atggggggta tacccggatc tgaccgacct
     2401 gaccgattca cccgacatct tgaacctgac cttcaaggtg aaagccctgg cctccgatcc
     2461 gctcaacgaa gaacacgaca atccccgcgt ccggcgtcca gttgcgcgca cgcttgcgac
     2521 cggcctatcg ggaagacacg ttctacctgt cgccctggta cgagtccgat gagaaggggg
     2581 acgtcgaaat tcaggtcggc ggcggcggcg cggtgatcga accgatcaac gacctctggg
     2641 tccgtcctcc gtttgccgtg ctcgaggccg acggcggtgc ggtcggttat ggcgagaacc
     2701 gcttgaaaat cgaggacctg tggacgttca agggaacgct caagatccgc gtcaaggatc
     2761 cggccggacg ccccgtcacc ggcgtgaggg tgagtcttga aggagcggtc caggccttca
     2821 gcctttacag cgatagtgcc ggggaagtcg acgtgaccgt gaacggcggc gggtccgaca
     2881 cgcgcaccgt ggagctgacg gtcgtgccgc gcatgctgct ggacagttcg ctgacgcaga
     2941 tcgtcgacaa cgtccaggag agccagctcc acggcaatac gcccgtgttc tacgtggcga
     3001 aattcaggct cgacgattcg atcaactacc tgacatccgg cgatccattt gacgacttca
     3061 cgctcaacat ttatgagaac gacttgctga tcgcccagat cgacggcctg acggccaaat
     3121 gcgccgcggc gatgacgccg gcagatcatg cccggggagt caatgcctat cactacccgg
     3181 tcctgcccga ggccaccccc aagtcgtacc gccatcagat cgtctggaaa aacgcgctgg
     3241 gggaagtcat gccgctgtcc cccctgtggt cgccgcgcac gatccgcgaa aggcgcgttc
     3301 ccgcgcttga gcggccgttt cggattcatt atgtcggcat cgacacgaag ggcgcctaca
     3361 aggcccacgg acgcaacatc atggagacct gggcctcacc ggcgcgcatg gccaagataa
     3421 acgattattt ccagatgctg tatcccggac cggtggagtt cacctatggc ccggacctgg
     3481 tagcgagcga accctggtat accttcgggc cctaccaggt gctctcgtat ttcgtggacc
     3541 tggcccgcct gcgcgagtcc atgccggacc gacccgacct gatcgtcggc gtatcggccg
     3601 cgggcgtcat cggcgccgac ggtctctccg agccccgctt ccgggaagtc atcctgctcg
     3661 atggctgctc gatgcgcgaa agctacctgc tgcacgaata cctgcacacg ctgggtctgc
     3721 tcgacaatta cgaatacgag acgggagaag ggacatacgg cgccagcgcc aatggctacg
     3781 acccgcggac gatgcggcgc atctacaatc cgcccggcgt cgacaagccg ccgcaccaga
     3841 cggtgatgta cgaccgcgcc cccagcccct ggccgacgac cgagggctac agcaaattgt
     3901 tggacgaggc cacggtcgcc ttcatcgaca acgcgaagga ttcggcgcga gcggaagaaa
     3961 aggccatcga accgcttgcg gcccctcccc aggaagtcat gctgatcggc ggggtgtacc
     4021 tcggtcgcca gtcctgggcg gactactggt atacgatgcg ggacgtctgg ccgatttttg
     4081 tggatacgga tgtggcctgg cagccgcagc aaacggcgac gtcgaatgcg cgcgccctcg
     4141 gcatccggct ctccaatggt caaaccgcgt cggtcctcga ggacagatat tggatctggg
     4201 gggcgtatga tgggcaggaa gcggggcagc tggcggccgc gccgctgttc aagattccat
     4261 acgatccggc gacgaacgcc gttaccttcc aggctcagta cactggcgac cttgtgcgca
     4321 cgattcgaga ggttcccttc tcgccccatg cgccgacggt cagcatcgtc tcgccggcgg
     4381 cggggagccg gctggcgggc catgccccgc ttgtgttcca ggccgacgat gtcgatgccg
     4441 gcgaacaact ctatgcctgg atcaagctga gcggcgacgg cggcgcgacc tggaccccag
     4501 tgggcaactg gttcccgatt ccccaaaggc agaacacgtt tgccctgccc tgcgacgacc
     4561 tgcccacgct ggacgccgcg cggctgcgcg tcctgatttc ggacggcacg cgatcggcaa
     4621 gggccgatgc gggccctttt gcgctcgatg gctatatgcc gggaccgcgg gcagcggccg
     4681 atccggcctg gcatgaatgg acggtgcgca cgggatcgcg ttgcgattta cccgtgaaaa
     4741 tcgtcaattc cggccgcgcg gcgcttgagg tgagtttcga tgccggcgcg aagccgtcat
     4801 ggctgacatg ccgccagctc ggcaagcaat cgatcgtcgc gggcggctcg ttcgagtttc
     4861 tgttcgagtg cgcggccgga gccgcgggag aacagaacgc cgaactgcgc ttcctgacca
     4921 atgatgcgca acaacccgtg ttgaccgtgc cgttgaagct gcgcctggtc gagacggcca
     4981 ccgcccccgt ggtggcgacc gtcgccaccg atccgccggc cgatgccggc gaacgtacgg
     5041 ccttgcgcaa tacgccggtc cggttcgtgg tgcgcgaggc ctgcggccgc gccggcctgg
     5101 ccggacgcat cgaaattcat cacgccgaga cgggcgaggt gctggcggtc gtcgatctgg
     5161 cgcccggcac ggtgcccggc ctttaccagg cggaatggga tgtgccggcc gggtgcttcg
     5221 gcgtgcccct gggtgtcgag gcgacgctga ccgacagcgc gagtgggctg agcagcagcg
     5281 ggggcagcaa tcccgtcggc tgggatgcgc gcctgcaact gatgcgccgc aacaccgcgc
     5341 cgcgtttcat caatccgacg aacgacaatt acgtggacct catcatcccg caaaccctgg
     5401 agattccctt cgaggcggtg gacgatgagg gggatccgat ctcattcgcc gtgcgacaca
     5461 ccagcgatct gaatgtccgc ctcgatgcgg aagcccggaa gctctacgtt gcgttgttgg
     5521 tggacgccca gacgccgctg tcgcaaatct ggttggccgc gtgcgatccg tggggcgcca
     5581 ccagcggggt ggccctcacg atccgagttg gctggcagaa tacgaactac ggtcacctgg
     5641 cctacctctc caatttcctg cttgaaacca atccgatgcc gctggcgatt gccggcggct
     5701 ggtccatgga gtggccccag aaggtggtgc tcgactatcg cgctgaaggg gcgccggcgt
     5761 ggacccgact cggcgagtat gaattcaccg aatgggataa tctcaagaac tgctggattg
     5821 cgcattgtct ttgggactgg cccaccgacg gcacagccgt ctttgaactg cgggtcacgt
     5881 tggtcgggag caacggcagc gccgaccccg agccgcccgt ctatcgcttc gagcgcccgc
     5941 gccagggggg acgggtgacg cgtgtcgaag cgccctcccc cgtcatggcc ggcgcggagt
     6001 tcaccctccg gatccatgtc gcgaacgact ccagcgaccg ctggtcggcg gaatcgggcc
     6061 atgggctgat cgccctggac ggggtcgatc cgctcacggg gctggccacg gccgaattca
     6121 tgggtctcca atccatcgaa cgcggctcga acggggtggt tgaggtccgc gccacggccc
     6181 cggccgcacc gggcgtctat cggaccgcct ggggcttgcg cgtcggttcg acgccgcttg
     6241 gcacgggggc cgaagccacc gtgcaggtgg tcgcgcgcgc ggccgacgcc ctcgcggcct
     6301 cgaccttgat cgacgtgctt ctgggccggg tcgccatgca gtcgctgatt cccggtacac
     6361 tggatctgaa cggcgacggt caaatcgacg tggccgacct ggtcctgctc ctgaaccgat
     6421 cggccggggg aggcgtctga tcccgcggcg cagtgcaccg cggcacggat tgcgaacccg
     6481 cacccgcccg cttacggccg acggccggtg cgggcgggcc gcgcgtgcag gatgaccagc
     6541 ccgatcgcgc cgcccagaat caacgctccg ccgcccagtt ccttccctgc cagaatccgc
     6601 cccagccacc accaggccat cggcaggacg aacagcggct cgatcgtctg aacgacgctg
     6661 gtgggggcca cgcc
//
LOCUS       DAOPCO010000221         6669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10207_length_6669_cov_5.755216, whole genome shotgun sequence.
ACCESSION   DAOPCO010000221 DAOPCO010000000
VERSION     DAOPCO010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6669
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10207_length_6669_cov_5.755216"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1769)
                     /gene="guaA"
                     /locus_tag="PLS90_13250"
     CDS             complement(237..1769)
                     /gene="guaA"
                     /locus_tag="PLS90_13250"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPK03411.1"
                     /translation="MILILDYGSQYTQLIARKIRSLNVRSEIVPFDWPLAKVRAARPD
                     GLILSGGPSSTYAPGAPRLNMKLLELGVPILGICYGMQLLTRKLGGVVERGTVREYGR
                     ASLNLSPAAAKDRLLKGIDSSTQVWMSHGDRVESVPAGFETLASSADCRFAAVRDRRR
                     PIWGVQFHPEVHHTPRGVQMLANFCFEICGARADWKMGRFVEETIEKLRAQTAGREVI
                     CAVSGGVDSTVLAVLLHQAIGPRLRPVYVDHGLMREGESEKVVGRFRDQLGIRVKQVR
                     AGRRFLHELKDVADPERKRKIIGREFVKVFFNEFRADEMLAQGTLYPDVIESVSTKGP
                     SATIKTHHNRVPEIQRLIRQGRVVEPLAELFKDEVRAVGHELGIPHDLLWRHPFPGPG
                     LAIRILGVVTPARLKLLRACDRIYLEELRRADLYKDIWQAFCVLLPVKSVGVMGDERT
                     YAYVVALRAVTSVDGMTADWFDMPQPVLARISNRIINEIDGVNRVVYDVSSKPPATIE
                     WE"
     gene            complement(2035..4170)
                     /gene="rnr"
                     /locus_tag="PLS90_13255"
     CDS             complement(2035..4170)
                     /gene="rnr"
                     /locus_tag="PLS90_13255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPK03412.1"
                     /translation="MGKRHTKAKSDRQAPQIGDLVTGIFQAGGRRGGYVVPDTAGLED
                     LRIPPMSAGAALHGDTVQARLTRLKEGRQPRAQIVKIIKHANKRLIGRLSYGGGKAFV
                     HPKNAKIKRYVELRGRLDPAEAPDGVWVVVEIRQWSSSPDEPLLGRLLEVLGSDDEPG
                     LPILLLVRSGGIEIEFPAEVEREAQRLLEAAPPDEPGGPSRARRRDFRGRRVITIDPA
                     TAKDFDDAVELIEVLPDGWRVGVHIADVAHYVRPGTALDAEAYERATSIYPVDRVIPM
                     LPEALSNQLCSLRPGEDKLTMSALFKVDRHGQVSEVELCNSLIHSVRRFAYEEVQGLF
                     DVEDLATGAREEPRRPPLPAPAVPDALLDDLMELRLAGRALREARMRRGALDLDLPEP
                     EILFDADRRVVDLRYAERHEAHQLIEELMIAANEAVARELEKRSLPLLFRVHDEPSED
                     KLLVIGPVLARLGIPVPAHGGMSRAELQHALEKARAHPAGIVVQRWVLRAMMRAKYQP
                     ENLGHFGLASESYCHFTSPIRRYPDLIVHRVVKAMLRGARAADEEMQALREELPARGR
                     HTSAREERAQRAEWDAQEILALQYMKRYLGDIFEGFIAGVGPMGFFVALKDYPVEGLV
                     RISQLDDDFYDLDDVRHIWQGRRSGRTWALGDPVSVMIERIDVLAGQMDLILVRQGRK
                     PARGGATGSAGPRTGGGGRRGGRWGRRRD"
     gene            4289..5428
                     /locus_tag="PLS90_13260"
     CDS             4289..5428
                     /locus_tag="PLS90_13260"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPK03413.1"
                     /translation="MSLPAHDAPPTDLSRALPRAAAVIEQGRRDGLHIGAQLYVSRHL
                     EVLADAALGEARPGAPMRTDSIMPWLSAGKPLTAVAIGRLWEQDALDLDDPVARHIPE
                     FAANGKEAITIRHLLTHTAGLRWADVRWPKDSWDEIIATICEARPEPRWRPGVTAGYH
                     LATTWFVLGELVRRLDGRMIDRYVRETIFLPLGMTDSWIGIPREQVRAYGERLGVLMN
                     TEDTPAVPQWTDPQRVIEPAPGNNARGPARELGAFYEMLLARGQAGGVRILRPQTVEA
                     LTSPNRVGRMDKTFQCVIDWGLGFIVNSRHYTGGAIPYGFGPHAGRRTFGHGGAQSSI
                     GFCDPDNGLVVVAIFNGRPGEQKHQARNEALNRAIYEDLGVAGED"
     gene            5494..6573
                     /gene="lepB"
                     /locus_tag="PLS90_13265"
     CDS             5494..6573
                     /gene="lepB"
                     /locus_tag="PLS90_13265"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPK03414.1"
                     /translation="MQTCPRCGFHNMDREARCVRCRAFLGRINDDDPLAPVDREAPRV
                     ELNRRGWTPIFVQRATTALLLALHRLGRALQDRPAPPELERRKAWLAGLLSMVPGLGQ
                     LYNRQPRKAVLFMLLVAAAWAIALANLYRPWSNGLLLAGLAAIVWSCNDAFISAQRIN
                     GATVLRQHALGFFIAWFFYIAIGCLLIQYVAGKTLFNLVAVISDEAAPYLRRGERYVA
                     SRIVFRLRDPRLGDVVVYRPPLARIERGEDLYLVKRWNGVERVVGLPGDTFSQRDGVF
                     YRNGVPAPVAEQPVVQRQIGHDFKIVAPPDRFIIVYSYSTAQAPGIQQAIYDHWEELC
                     LVPREDILSKPWFVYHPPAARRMLR"
BASE COUNT         1040 a         2437 c         2084 g         1108 t
ORIGIN      
        1 aagtctgccg cccctgacgg gactgtcagc ggaccgagac cgaccaggcc tggccgttag
       61 tttcggtcag ttgcagcctt ccgtcttcct cccttgcgtt gaaccgccga ttgttgagca
      121 actcgttcgt tctcacgctc ggcgccgatc gccaaacccg acgatcttcc aattgccgcg
      181 gtgaaaactc aagcggctat ccgcccgaga gcgccgctcg ggctgggcgc aaggcctcat
      241 tcccactcga tcgtcgcggg gggtttactg gagacgtcgt agacgacccg gttgaccccg
      301 tcgatctcgt tgatgatgcg gttgctgatg cgcgccagga cgggttgcgg catgtcgaac
      361 cagtcggccg tcatgccatc gacgctggtg accgcgcgca gcgcgacgac gtaggcatag
      421 gtccgttcgt cgcccatcac gccgaccgat ttgaccggca gcaggacgca gaacgcctgc
      481 cagatgtcct tgtagaggtc ggcgcggcgc agttcctcca gataaatccg gtcgcaggcg
      541 cgcagcagct tgagccgtgc gggcgtcacc acgcccagga tgcggatggc cagacccggc
      601 cccgggaagg gatgacgcca gagcaggtcg tgcgggatgc ccagttcgtg ccccaccgcg
      661 cgcacctcat ccttgaacaa ctccgccagc ggctcgacga cccgcccctg ccggatcaac
      721 cgctggattt cgggcacacg gttgtggtgc gtcttgatcg tggccgaggg ccctttggtc
      781 gataccgatt cgatcacatc gggatagagc gttccctggg cgagcatctc gtcggcccgg
      841 aattcattga agaacacctt gacgaactcg cggccgatga tcttgcgttt gcgttccgga
      901 tcggccacgt ccttcagctc gtgcagaaac cgccgcccgg cgcgcacctg cttcacgcgg
      961 atgccgagct ggtcgcggaa ccggccgacg actttttcac tctcgccttc gcgcatcagg
     1021 ccgtggtcga catagaccgg acgcagccgc ggtccgatcg cttggtgcag cagcaccgcg
     1081 agcaccgtcg aatcgacgcc ccccgagacg gcgcagatca cctcgcgccc cgcggtctgc
     1141 gcccgcagtt tttcaatcgt ctcctccacg aaccggccca tcttccagtc ggcgcgggcg
     1201 ccgcagatct cgaaacagaa attggcgagc atctgcacgc cgcgcggcgt gtgatggacc
     1261 tcggggtgga actggacgcc ccagatcggc cggcggcggt cgcgcacggc ggcgaagcgg
     1321 caatcggccg aggaggcgag ggtttcaaag ccggccggaa ccgactcgac ccggtcgcca
     1381 tggctcatcc aaacctgcgt cgagctgtcg atccccttga gcagcctgtc cttcgccgcc
     1441 gcggggctca ggttcaagga ggcgcggccg tactcgcgca ccgttccgcg ctccaccacc
     1501 ccacccagtt tgcgcgtcag caactgcatc ccgtagcaga tgcccaggat cggcacgccc
     1561 agctcgagca gcttcatgtt gagccggggc gcccccgggg cgtaggtcga actggggccg
     1621 cccgacagga tcaggccgtc gggccgggcg gcacggacct tcgccagcgg ccagtcaaac
     1681 gggacgatct cgctgcgcac gttgagcgag cggatcttgc gcgcgatcag ttgcgtatac
     1741 tgcgaaccgt aatcgaggat gaggatcatc ggagcccccc gccacggcgg cctgcgagcc
     1801 ggccgaagga tcggccttgc cgcctcccct tgcgaggggc acggataccc cccgctgggg
     1861 gaaacgaaga gcataccgaa gcgccgcggc gccggtcaag ccgccctcca tccccactct
     1921 ttcgtcccac gtcccacgtc ctggtcctcg tgaccgctcc cacaggccca ctccagattc
     1981 tgtcccttgc tgtgccccac cggtcatgag caatccgtga atggccgcgg aacgttaatc
     2041 ccggcgcctt ccccagcgtc cgccgcggcg gccgccgccg ccggtgcgcg gtccggccga
     2101 ccccgtcgcc ccgccccttg ccggcttccg tccctggcgc acaaggatca gatccatctg
     2161 ccccgccagc acgtcgatcc gctcgatcat cacggaaacc gggtcgccca gcgcccaggt
     2221 acgccccgag cgccgccctt gccagatgtg ccggacgtcg tccaggtcgt agaaatcgtc
     2281 gtcaagctgg ctgatccgga ccaggccttc gaccggataa tccttgagcg cgacgaaaaa
     2341 gcccatcggc ccgacccccg cgatgaagcc ctcaaaaatg tcgcccaggt agcgcttcat
     2401 gtattgcagc gccagaattt cctgcgcgtc ccactcggcg cgctgcgccc gctcctcgcg
     2461 cgccgaggta tgtcgccccc gcgccgggag ttcctcgcgc agcgcctgca tttcctcatc
     2521 cgccgcccgc gccccgcgca gcatcgcctt caccacccgg tgcacgatca gatcgggata
     2581 gcgccggatc ggcgacgtaa aatgacagta gctctccgac gccagtccga agtggcccag
     2641 gttctccggc tgatatttgg cccgcatcat tgcccgcaac acccagcgct gcacgacgat
     2701 acccgccgga tgcgcgcgcg ccttctcgag cgcatgctgc agttccgccc ggctcatgcc
     2761 gccatgcgcg ggcacgggaa tccccagccg cgccaggacg ggcccgatga ccagcagctt
     2821 gtcctccgag ggttcgtcat ggacgcgaaa caggagcggc aggctgcgtt tctccagttc
     2881 gcgcgccacg gcttcattgg cggcgatcat caactcctcg atcaattggt gcgcctcgtg
     2941 gcgctccgca tagcgcagat cgaccacccg ccggtcggcg tcgaacagga tctccggctc
     3001 gggcaggtcc aggtcgagcg cgccgcgccg catccgtgcc tcgcgcagcg cccgtccggc
     3061 caggcgcagc tccatcaggt cgtccagcag cgcatcgggc acggccgggg ccggcagcgg
     3121 cgggcggcgc ggttcctcgc gggcccccgt cgccagatcc tccacgtcga acagcccctg
     3181 cacctcctca taggcgaacc gccgcaccga atgaatgagc gagttgcaca actcaacttc
     3241 gctcacctgg ccgtggcgat ccaccttgaa cagcgccgac atcgtcagct tgtcctcgcc
     3301 cggccgcagg ctgcagagct ggttggagag ggcctcgggc agcatcggga tgacgcggtc
     3361 gaccggatag atcgacgtgg cgcgttcgta ggcctcggca tcgagcgccg tccccggccg
     3421 gacataatgc gccacatcgg cgatgtggac gccgacgcgc caaccgtcgg ggagcacctc
     3481 aatcagctcg accgcgtcat cgaaatcctt ggcggtcgcc ggatcgatcg tgatgacgcg
     3541 ccggccgcgg aaatcgcgcc gccgcgcccg gcttggtccg cccggctcgt cgggcggcgc
     3601 cgcctccagc agccgttgcg cctcccgctc cacttccgcg gggaactcga tctcgatccc
     3661 gccggagcgc accagcagca ggatcggcag ccccggttca tcgtcgctcc ccagcacctc
     3721 cagcagtcgc cccagcagcg gctcgtcagg cgaactggac cactggcgga tctcgaccac
     3781 cacccagacg ccgtcgggcg cctcggccgg atccagccgc ccgcgcagct ccacgtagcg
     3841 tttgatcttg gcgttcttgg gatggacgaa ggccttgccg ccgccatagc tcagccggcc
     3901 gatcagccgt ttgttggcgt gcttgatgat cttgacgatc tgggcgcgcg gctggcgccc
     3961 ctccttcagc cgcgtcagcc gcgcctgcac cgtatcgcca tggagggccg ctccggcgct
     4021 catcggcggg atccgcaagt cttccagacc cgcggtatcg ggcacgacgt accctccccg
     4081 ccgcccgccg gcctgaaaaa tgcccgtgac cagatcgccg atttgaggcg cctgccggtc
     4141 ggatttcgct ttcgtgtgac gcttgcccat gctggctccc cgatgagaat gcggcgcctg
     4201 ggccaatcca aagctgccaa cccgcttccg ctctgctaac atcgtgccgc ttttgtgtgc
     4261 gaatgtctaa aagggatgtt gcccgccgtt gtcgctgccc gcccatgacg ctccgcccac
     4321 ggacctcagc cgggcgctgc cccgcgccgc ggccgtcatc gaacaggggc ggcgcgacgg
     4381 cctgcatatc ggcgcgcagc tctacgtgtc gcgccacttg gaagtgctcg ccgacgccgc
     4441 cctcggcgaa gcccggcccg gcgcgccgat gcggaccgat tcgatcatgc cctggctttc
     4501 cgcgggcaag ccgctcaccg ccgtcgcgat cggccggctg tgggaacagg acgccctcga
     4561 cctggatgat cccgtcgcgc gccacattcc ggaatttgcc gccaacggca aggaagcgat
     4621 tacgatccgg cacctgctga cgcacaccgc cggcctgcgc tgggccgacg tgcgctggcc
     4681 caaggattcc tgggatgaaa tcatcgcgac catctgcgag gcgcggcccg aaccccgctg
     4741 gcgaccgggc gtcaccgccg gctatcacct cgccacgacc tggtttgtgc tgggcgaact
     4801 cgttcgccgg ctggacgggc ggatgatcga tcgctacgtg cgtgaaacca tcttcctccc
     4861 gcttggcatg accgacagtt ggatcgggat cccgcgcgag caggtccgcg cctacggcga
     4921 gcggctcggc gtcctgatga acaccgagga cacgcccgcc gttccgcaat ggaccgatcc
     4981 gcaacgggtc atcgaaccgg cccccggcaa caatgcccgc ggtcccgctc gcgaactggg
     5041 cgcgttctac gagatgctgc tcgcccgggg acaggccggc ggcgtccgta ttctgcggcc
     5101 gcagacggtc gaagcgctca cctcgcccaa ccgcgtcggc cgcatggaca agaccttcca
     5161 gtgcgtgatc gactgggggc tcggtttcat cgtcaactca cggcattata ccggcggcgc
     5221 gattccctat ggctttggtc cgcatgccgg ccgccgaacg ttcggccacg gcggcgccca
     5281 gtcctcgatc gggttctgcg acccggacaa tggcctggtc gttgtggcga tcttcaacgg
     5341 ccggcccggc gagcagaagc atcaggcgcg caacgaggcg ctcaaccgcg cgatctatga
     5401 agacctcggc gtcgccggcg aggactgact gcaacccgaa ccggcgttcg cgagacttca
     5461 gcttcaggcg tccgggatcg ggaggcttgg ccgatgcaga cttgcccccg ctgcgggttt
     5521 cacaacatgg atcgcgaggc gcgttgcgtg cgctgccggg ccttcctggg ccggatcaac
     5581 gacgacgacc ccctggcgcc cgtcgaccgg gaggcgccgc gcgtcgagct gaaccgccgc
     5641 ggctggaccc cgatcttcgt ccagcgggcc acgaccgccc ttctgctcgc cctccaccgc
     5701 ctgggtcgcg cgctccagga tcgccccgcc cctccggagc ttgaacggcg caaggcctgg
     5761 ctggccggcc tgctgtcgat ggtccccggt ctgggccagc tctacaaccg ccagccgcgc
     5821 aaggccgtcc tgtttatgct gctcgtcgcc gccgcgtggg ccattgccct ggccaacctg
     5881 taccggccct ggtcgaacgg gctcctgctc gcggggctcg ccgccattgt ctggtcgtgc
     5941 aacgacgcgt tcatcagcgc gcaacgcatc aacggcgcca cggtgctgcg gcagcatgcg
     6001 ctggggttct tcattgcctg gttcttctac atcgcgatcg gctgcctgct gattcagtat
     6061 gtcgccggca agacgctgtt caacctggtg gcggtgatca gcgacgaggc cgccccctac
     6121 ctgcgccgcg gcgaacgcta cgtcgccagc cggatcgtct tccgcctgcg cgatccccgg
     6181 ttgggcgacg tggtcgtata ccggccgccg ctggcccgga tcgaacgagg cgaggacttg
     6241 tatctcgtca agcgctggaa cggcgtcgaa cgcgtcgtcg ggctgccggg cgacacgttc
     6301 tcccagcgcg acggggtttt ctaccgcaac ggcgttcccg cgccggtcgc cgagcagccg
     6361 gtcgtgcaac ggcagattgg ccacgatttc aagatcgtcg cgccgcccga tcgtttcatc
     6421 atcgtctact cctactcgac ggcgcaggcg cctggcatcc agcaggcaat ctacgatcac
     6481 tgggaagagc tgtgcctcgt gccgcgcgag gacatcctca gcaagccgtg gttcgtctac
     6541 catccgcccg cggcgcgacg gatgctgcgc tgagggattt tctttcattc gcgccgcgaa
     6601 ttggctagcc tcgcaggatt aagccgcccg ccccaagccg acggcatgag acctccccga
     6661 gaccccgca
//
LOCUS       DAOPCO010000222         6594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10331_length_6594_cov_4.197890, whole genome shotgun sequence.
ACCESSION   DAOPCO010000222 DAOPCO010000000
VERSION     DAOPCO010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_6594_cov_4.197890"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLS90_13270"
     CDS             <1..1191
                     /locus_tag="PLS90_13270"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPK03415.1"
                     /translation="RNGVQPRVLPGGFGLKDAIADELDELYVSDIVQRIRDNDYQWEV
                     NGLRFRLAREFGFCYGVDKAIDLSFETRRRFPDRRLFLTNEIIHNPRVNHRLLEMGYQ
                     FLQGQYAAGNELDSIRPEDVVVLPAFGIEQRLLARLRQIGCILVDTTCGSVVHVWKRV
                     ERYAREGFTSIIHGKWRHEETVATCSHVTQHPGAHYLVVFDKAETERVCDVIRTGRFS
                     EQLARDFVSAASPGFRFDQHLSRIGVANQTTMLSSESLEIAEMIRQAMIDRHGAAYAA
                     ENFRSFDTICNATQERQDAIKEMMQHPPDLMLVIGGFNSSNTGHLCELTAPHCPTYHV
                     DDVQRLISPTAIEHKPPMAHDLVVTHDWLPPRRPLEIGLTAGASTPNRVIGEIIERIL
                     SWSS"
     gene            1381..1875
                     /locus_tag="PLS90_13275"
     CDS             1381..1875
                     /locus_tag="PLS90_13275"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HPK03416.1"
                     /translation="MASQVVTAEKRYRISLLLDAYGELLTDKQRTFLRHYFEEDLSFG
                     EIAREYGVSRQAIFDSVKHGEESLEHFEQVLGLVRGGWNAWTAQNLSPRAVAQRLEEA
                     LHLLNGHGGSPAGHDALEQALRAVIRELTAPPADEEPVPPPASSPTPVPMTVAGSTQS
                     PMEQ"
     gene            1886..3337
                     /gene="ffh"
                     /locus_tag="PLS90_13280"
     CDS             1886..3337
                     /gene="ffh"
                     /locus_tag="PLS90_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPK03417.1"
                     /translation="MFDQLGEKFEGLFKKLRGETRLTEDNIQEAVREVRMALLEADVN
                     FKVVRDFVAAVREKALGAEVLRAVRPGQQFIKIVHDELVAMMGGAAVKLQLQRGRLNR
                     VVLLGLQGSGKTTFAGKLALRLKKEGFSPLLVACDIYRPAAVDQLRVVGATVDVPVFD
                     LGTSQPAPVVAERGLAEAKNRNCDLVIFDTAGRLHIDEVRMDELQRIKELTKPDYAFL
                     VADAMTGQDAVNSAAQFDEQVGIDGVCLTKLDGDARGGAALSIRSVTGKPIYFVGLGE
                     KPDDLEIFHPDRMASRILGMGDVLTLVEKAQQDFDADEAERMQKKLKKEQFSLQDFLD
                     QMQKVKKMGSLKSLMGMIPGLGSALRDVEIDDDMLKPFEAMIHSMTPEERENPQIIDG
                     SRRKRIARGSGRTPAELNQMLQEFQGMRRMMSQMMNMGGPGGLMRGMLGGGAPAMAGA
                     RRGGALNQKSADDPLARKMKPKHRRKRKTKKKK"
     gene            3461..3778
                     /gene="rpsP"
                     /locus_tag="PLS90_13285"
     CDS             3461..3778
                     /gene="rpsP"
                     /locus_tag="PLS90_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPK03418.1"
                     /translation="MAVRIRLARHGRKKAPYYRMVVADSRFPRDGRFIEILGYYHPLE
                     KDEEKQVKVDEEKALMWLDRGAIPTDTCRSLLRKQGILRKHHEAKQAARLASKAASAP
                     AEA"
     gene            4128..4358
                     /locus_tag="PLS90_13290"
     CDS             4128..4358
                     /locus_tag="PLS90_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPK03419.1"
                     /translation="MKELVDYIARALVDFPDKVEVIAMESENSVIIELKVAPEDVGKV
                     IGKGGQTAKAMRKILSAAATKLHKKSLLQIIE"
     gene            4700..6121
                     /locus_tag="PLS90_13295"
     CDS             4700..6121
                     /locus_tag="PLS90_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03420.1"
                     /translation="MSDPTNRPHAPEKPPVRDFALECIQLGPSTRIIALELDRQGVLF
                     YQFRRNRPQSIHLTPSYAGQSVVLIDMVTAAALLHPVTHRLLAYAGFLSDGTRSFLFG
                     RDWGKWVNDAGELFRTHDVPDLMRELRLPISLQDTKHLVAFDFLINPHDREHLTREVV
                     TLVRDARMGYVLHLLFRYPSGEYQKARLSLHEANSHRLEYVYDLAEFPVKLPAPGRVP
                     SGRSTTDRIEKLRQSQFTKLKRPAAAPDGAEGEEAPVTYSPALLGLPEDKRNHRAAAV
                     EPARSQPVPDTLNLSTHSLLGVLSTEPDPDRRWRMIEPNLMLGRPERNYPLILNYPEL
                     IAERTRDWPPPRLKAAFGNQPGSVLIGIMEHWSLNQILAVAEDNAESQRIIEWVREHE
                     IARLLKLDTKEEPTSAEAARRILRVDRNADARMIRKVWRTLLHTLNADLGRQEERAIH
                     RRKDEIAKHLQVARDVLMRSSHP"
     gene            6329..6511
                     /gene="rpmG"
                     /locus_tag="PLS90_13300"
     CDS             6329..6511
                     /gene="rpmG"
                     /locus_tag="PLS90_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002662366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPK03421.1"
                     /translation="MAKQQNRVQVIMECTECRQSGQPGVSRYVTTKNRRTTTSRVELK
                     KYCKFERKHTLHREVK"
BASE COUNT         1239 a         2376 c         1877 g         1102 t
ORIGIN      
        1 aggaatggcg tccagccgcg ggtattgcct ggcggcttcg gcctgaagga tgccatcgcc
       61 gacgaactgg acgaactcta cgtctccgac atcgtccagc gtatccgcga caacgactac
      121 caatgggagg tcaacggcct ccgcttccgt ctggcgcgcg aattcggctt ctgctacggc
      181 gtcgataagg cgatcgacct ctcctttgaa acgcgccgcc gcttccccga ccgcaggctc
      241 ttcctcacca acgagattat tcacaacccg cgtgtcaacc accgcctcct cgagatgggc
      301 taccagttcc tccaggggca gtatgccgcg ggcaatgaac tcgattcgat ccgccccgag
      361 gatgtcgtcg tcctgcccgc cttcggcatc gagcagcgcc ttctcgcccg cctgcgccag
      421 atcggctgca tcctggtcga tacgacctgc ggctcggtcg tccacgtctg gaaacgggtg
      481 gaacgctacg cccgcgaagg cttcacctcc atcatccacg gcaagtggcg gcacgaagag
      541 accgtcgcca cctgctccca tgtcacccag caccccggcg cgcattatct ggtggtgttc
      601 gacaaggccg aaaccgaacg ggtctgcgac gtcatccgta ccggccgatt cagcgagcaa
      661 ctcgcccgcg atttcgtctc cgccgcctcg cccggcttcc gcttcgatca acatctgagc
      721 cgcatcggcg tcgccaatca gacgaccatg ctttcgtccg aatcgctcga gatcgccgag
      781 atgatccgcc aggccatgat cgaccgccac ggcgcggcgt atgccgccga gaatttccgc
      841 tccttcgaca cgatctgcaa tgccacccag gaacgccagg acgccatcaa ggagatgatg
      901 caacaccccc ccgatctgat gctggtgatc ggcggattca atagctccaa caccggccac
      961 ctatgcgaac tgacggcccc ccactgcccg acctatcatg tggacgacgt ccagcgcctg
     1021 atctccccca ccgcgatcga gcacaagccg ccgatggccc acgatctggt cgtcacgcac
     1081 gactggctcc ccccgcggcg gcccctcgaa attggcctga ccgccggcgc ctccaccccc
     1141 aatcgcgtca tcggcgagat tatcgaacgc atcctgtcct ggtcgtccta gctccctccc
     1201 caatcaagat cggtatcggt atcgccaccg ctttcgcaat cgatcctgat cgcgatgacg
     1261 atttcggcaa cttcgccgcg cttgacagtc actctggcga ctcgctagag tgttagcccg
     1321 catgtaaagc atcaggcttt gacatgcccc ctggcgggtc cagtccctgg agtgcttccc
     1381 gtggcttccc aagtcgtgac ggccgaaaaa cgctatcgca tcagcctgct gctcgacgcc
     1441 tacggcgagc tgttgaccga caagcagcgc accttcctcc gtcactattt cgaggaggac
     1501 ctctccttcg gcgaaatcgc ccgcgagtac ggcgtctcgc gtcaggccat cttcgattcc
     1561 gtcaagcatg gcgaggaatc gctggagcat tttgagcagg tcctcggcct cgtccgcggc
     1621 ggctggaatg cctggacggc ccagaatctg tcgccgcgcg cggtggcgca gcgtctcgag
     1681 gaggcgctcc acctgctgaa cggtcatggg ggctcgcccg ccggccacga cgcgctggag
     1741 caggccttgc gcgcggtgat ccgcgaactg accgcgccgc cggccgacga ggaacccgtt
     1801 cccccgcccg cgtcctcccc tacgcccgtc cccatgaccg tggccggctc gacccaatca
     1861 cccatggagc agtagtccca ctggcatgtt cgaccaactc ggcgaaaagt tcgaaggcct
     1921 cttcaagaaa ctgcgcggtg aaacgcggct gaccgaggac aacatccagg aggccgtgcg
     1981 cgaggtgcgc atggccctgc tggaggccga cgtcaatttc aaggtcgtcc gcgacttcgt
     2041 cgccgccgtc cgcgagaagg ccctgggcgc cgaggtgctg cgcgccgtcc gccccggcca
     2101 gcaatttatc aagatcgtcc atgatgaact ggtcgcgatg atgggcggcg ccgccgtcaa
     2161 acttcagctt cagcgcggcc ggctcaatcg cgtcgtcctg ctcggactgc agggctccgg
     2221 caaaacgacc ttcgccggca agctcgcgct gcgcctcaag aaggaaggct tcagcccgct
     2281 cctggtcgct tgcgacatct atcgtcccgc cgccgttgat cagcttcgcg tcgtcggcgc
     2341 gacggtcgat gtccccgtct ttgatctcgg cacgagccag ccggcgcccg tcgtcgccga
     2401 acgcggcctg gccgaggcca agaatcgcaa ctgcgatctg gtcattttcg ataccgccgg
     2461 ccggctccac atcgacgagg tccgcatgga cgaactccag cgaatcaagg agctgaccaa
     2521 gccggactac gcgttcctgg tcgccgacgc gatgaccggt caggacgccg tcaactccgc
     2581 cgcccagttc gatgagcagg tcggcatcga cggcgtctgc ctgaccaagc tcgacggcga
     2641 cgcccgcggc ggcgccgccc tctccatccg ctcggtcacc ggcaagccga tttacttcgt
     2701 cggcctcggc gagaagcccg acgacctcga gatcttccac ccggatcgca tggcctcgcg
     2761 catcctcggc atgggcgacg tcctgaccct cgtcgaaaag gcgcagcagg acttcgacgc
     2821 cgacgaagcc gaacgcatgc agaagaagct caagaaggag caattctctc tgcaggactt
     2881 cctcgaccag atgcagaagg tcaagaagat gggctcgctc aagagcctga tgggcatgat
     2941 ccccggcctc ggctcggctc tgcgcgacgt tgaaattgat gatgatatgc tcaagccgtt
     3001 cgaggccatg attcactcga tgacgccgga ggaacgcgaa aacccccaga tcatcgacgg
     3061 ctcccggcgc aagcggatcg cccgcggttc ggggcgcacc cccgccgagc tgaaccagat
     3121 gctccaggaa ttccagggca tgcgccggat gatgagccag atgatgaaca tgggcggccc
     3181 cggcggcctg atgcgcggca tgctgggcgg cggcgccccc gcgatggccg gcgcccgccg
     3241 cggcggcgcg ctcaatcaga aaagcgccga cgacccgttg gcgcggaaga tgaaacccaa
     3301 acaccggcgc aagcggaaaa ccaagaaaaa gaaatgagtg cgcccctcgg ggatgaccgt
     3361 aaacaactga attttaacaa tttgccgagc ggagacgaaa gccccgcttt cgctccgtcg
     3421 gcgaaaccca ccccataagc cagcaaaagg agattgaccc atggctgtgc gtattcgact
     3481 ggcccgtcac gggcgtaaga aggcccccta ctaccggatg gtggtcgccg atagccgctt
     3541 cccccgcgac ggacggttca tcgagatcct gggctactac cacccgctcg agaaggatga
     3601 ggaaaagcag gttaaagtgg atgaggagaa ggcgctgatg tggttggacc ggggcgcgat
     3661 cccgaccgac acctgccgct cgctgctgcg caagcagggc atcctgcgca aacaccacga
     3721 agccaaacag gccgcgcggc tggcgagcaa ggccgcctcg gccccggccg aagcctgacg
     3781 cgcgccttcc gagtcctgat cggcggtggg gggccgcggg ctgccgcgcc ccaaacccgt
     3841 ccggcggctc ggtcatgcgc cggcccggcc cccgcgcggg cggatcggtt cgtcaacccg
     3901 atcgcgcggg acgcgcccgc cgcgccagcc cgctcgccaa aagctaacgc gaatgcactg
     3961 aatcttctag acacaatggg aaagttttga ttagctgaac acattgttcc cggcctgttc
     4021 cgatggttga cacgtgcaac ccgctggggt tgactcgtcc ccacgcagca cttcctagag
     4081 aaagcgggat ccagatgatg aagcaagccg aagccccgga aatcacgatg aaggagctgg
     4141 tcgactacat cgccagggcg ctggtcgatt tccccgacaa ggtggaagtc attgcgatgg
     4201 agagcgagaa cagcgtcatc attgagctga aggtcgcgcc ggaggatgtc ggcaaggtca
     4261 tcggcaaggg gggccagacg gccaaggcga tgcgcaagat cctctcggcc gccgccacca
     4321 agctccacaa gaagtcgctg cttcagatta tcgaatgagc ctgccctgat ctcacggcgc
     4381 cacccccgtg agctggatcg tttcggtacc cacggcctga tctccaaacg ctgacgcggt
     4441 tggccgggag ccccgcgttc gatctctata gtccgtccct cgtcttcgtg atcgccccca
     4501 aaggcccgcc ccagaactcg actcccggtt ctcgtcctcg gttctcaact gcccgcccag
     4561 gcccacggct ccattcttcc gtccccgcat ctccggccat ctccggctat ctcccgccaa
     4621 cgccccgggt ttccttttgc attcgatacc tgcatggtat atctagactg cacaccctag
     4681 gcccggtaat cggttgtcga tgtccgaccc cacgaaccgc ccccacgccc ccgagaagcc
     4741 gccggttcga gattttgctc tcgaatgcat ccagctcggc ccctccaccc ggataatcgc
     4801 cctggagctg gatcgccagg gtgttctctt ctaccagttc cgccgcaatc gcccccaatc
     4861 gatccacctg accccctcct atgccggcca atcggtcgtc ctgatcgaca tggtcaccgc
     4921 cgcggcgcta ctccatccgg tcacgcaccg gctcctcgcc tacgccggct tcctcagcga
     4981 cggcacgcgc agcttccttt tcggtcgcga ctggggcaag tgggtcaacg acgccggcga
     5041 actcttccgc acgcacgacg tccccgacct gatgcgcgaa ttgcgcctgc ccatctcgct
     5101 gcaggatacg aagcatctgg tcgccttcga tttcctgatc aatccccatg accgcgagca
     5161 cctgacgcgc gaggtggtca cgctggtccg cgacgcccgc atgggctatg tcctccatct
     5221 gctcttccgc tacccctccg gcgagtacca gaaggcgcgt ctctcgctcc acgaggccaa
     5281 ttcgcatcgg ctggagtacg tctacgatct ggcggaattc cccgtcaagc tgccggcgcc
     5341 cggtcgcgtt ccctccggcc ggtcgacgac cgaccgcatc gagaaactcc gccagtccca
     5401 gttcaccaag ttgaagcgtc ccgccgccgc gcccgacggc gccgaaggcg aggaagcccc
     5461 ggtcacctac tcccccgcgc tgctgggcct gcccgaggat aaacgcaacc accgcgccgc
     5521 cgccgtcgag ccggcccgca gccagcccgt ccccgacacg ctgaacctct ccacccacag
     5581 cctcctcggc gtgctttcga ccgaacccga tcccgatcgg cgctggcgca tgatcgagcc
     5641 caacctgatg ctcggccgcc ccgagcgcaa ctaccccctc atcctcaact atcccgagct
     5701 gatcgccgag cggacccgcg actggccgcc cccccgcctc aaggccgcct tcggcaacca
     5761 gcccggctcc gtcctgatcg gcatcatgga acactggagc ctgaaccaga ttctggcggt
     5821 cgccgaggat aacgccgaat cgcagcgcat catcgaatgg gtgcgcgagc atgaaatcgc
     5881 ccgcctgctc aaactggaca ccaaggaaga gcccacctcg gccgaagccg cccgccgcat
     5941 cctgcgcgtc gaccgcaacg ccgatgcccg gatgatccgc aaggtctggc gcacgctgct
     6001 ccacacgctt aacgccgacc tcggccgcca ggaggaacgc gccatccacc ggcgcaagga
     6061 tgaaatcgcc aaacatctgc aagtcgcccg cgacgtcctc atgcggtcga gccatccgta
     6121 gtcaaccccc gaaaacccct gtcgttccat cggatctgac cgatccgtcc gatccgaagg
     6181 atttcggtcg actcttcctt cagtcaccgt cgtttttcgc cgaaatgata tttgacagcg
     6241 cgacgccgta tgctatgcta tggcgtccct gtttgggggt gcggccgagc tgcatgaaga
     6301 gtcctcctcc cccggagtgt atcgtccgat ggccaagcaa cagaaccgag tccaggtgat
     6361 catggagtgc accgagtgcc gccagagcgg gcaaccgggc gtctcgcgct acgtcaccac
     6421 caagaaccgc cgcacgacga ccagccgcgt cgagctgaag aaatactgca agtttgagcg
     6481 caagcacacg ctgcaccgcg aagtaaaata gccggctcga atcccgcgac cccgcccggt
     6541 cgcgattcga cgccggcctg cccgcaaccc ccggattcgc cccgcgcgcc gggg
//
LOCUS       DAOPCO010000223         6592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10334_length_6592_cov_4.653052, whole genome shotgun sequence.
ACCESSION   DAOPCO010000223 DAOPCO010000000
VERSION     DAOPCO010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10334_length_6592_cov_4.653052"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1205
                     /locus_tag="PLS90_13305"
     CDS             198..1205
                     /locus_tag="PLS90_13305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03422.1"
                     /translation="MAKAQRPTANELQRDEVAESLQETLLTLQRNRKKLIAAAIVLIL
                     GVIIFTAVRSHRNSVIIESNRLMSDALEGYGRLIQATDETDRQREVDYTLAALDQLHD
                     SHGDTPLGHEALFLKGKIHYELGNWEEAQRAYQQYIDEARTAEQRAQGRIALAYAHEN
                     ASFSQPNEQARRSELDAALLNFEQAQDAAPDHSYLTYYAMLGEARYYELTGDNERAIA
                     VLERIVNERPAPVAQTDSADEADSPLDTELSEQLRTAVREQESQASFQATAKLRLERL
                     RARAGLEAPAPVTVVETAGGAPAADADAAAPGATQAEPDAADGVSTTEPAPPAASQAA
                     P"
     gene            1209..1865
                     /locus_tag="PLS90_13310"
     CDS             1209..1865
                     /locus_tag="PLS90_13310"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPK03423.1"
                     /translation="MFTGIIESVGVIRERLQTGGGLRLVVQPDRPLDALRAGESIAVN
                     GVCLTAEASSAPDRLSFFLSPETRRRSTLGDLPLGALVNLERALRAEDRLGGHLVLGH
                     VDAVGRIRRFDPQGESWLLEVGCPRELEPFLAPKGSIALDGISLTMVETGEDWFTVAV
                     IPHTAQATTLRRAVAGDAVNLEVDLLARYVVRALATLRARESGLSLQLLNKAGFLPSA
                     "
     gene            1924..2976
                     /locus_tag="PLS90_13315"
     CDS             1924..2976
                     /locus_tag="PLS90_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK03424.1"
                     /translation="MMAKAADTAPAPPDPRRACTLDQVLGHQAVRRYLRQAWRQRRLP
                     QAILLSGPEGIGKSTLAWALAREIAADGRDPATDPKSLKVVRGVHPDMFEISGKSTVS
                     RSIVVDEIRRLEDRAMTAPLEAPRKVFVIDPADRMNTAAANALLKILEEPHGRLAFVL
                     VTSVPHRLLDTIRSRCAELRLEPVAIDELAAWLRAHRPLDEPRARLLAALAEGRPGRA
                     LQWAGSRCLDARRQTIDALLLFHTHGFAAVFHVADRLAGAGDSLAEGIAVAVTLLRDA
                     LVLAMRGDGALNSDLVDNLRRLSEAFPPPGLLAAAARLEAAAAEAPYFYTPQAKAHFM
                     ECLATDVGKLLLTAPG"
     gene            complement(3004..4104)
                     /locus_tag="PLS90_13320"
     CDS             complement(3004..4104)
                     /locus_tag="PLS90_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HPK03425.1"
                     /translation="MSQDPQHVPRPESSRQAPRGRGAAGNPRNRFERVELEPFDEDLA
                     PEERLAPETLFLRDASKSIISYNDSPDVGFNASLNPYRGCEHGCSYCYARPTHEYLGF
                     SAGLDFETRILVKEDAPRLLREALASPRWKPQALALSGVTDPYQPIERRLQLTRRCLA
                     VLAECLNPVVMITKNAQITRDLDLLAELARRGCANVNITITTLDAELARRLEPRTSTP
                     RARLEAICRLREAGVPVGVLIAPVIPGLTDHELPAIVKAVADAGAGWARYILLRLPHG
                     LKELFSDWLERNEPLKRDKVLGRLRAMRGGKLYDSRFATRGRGEGSWADQIETLFRVA
                     HRRAGLGDPPALSCDAFRRPVVGQMELFGSGW"
     gene            complement(4166..5257)
                     /locus_tag="PLS90_13325"
     CDS             complement(4166..5257)
                     /locus_tag="PLS90_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HPK03426.1"
                     /translation="MARRKDLIIAGHKIPPGEVRDLQLPFSESYLGTPVGVPVRVIRA
                     PADGPRLFITGAIHGDELSGVGIIRDLLYDKPPELLRGAIVCVPVVNVYGLEHHSRYL
                     PDRRDLNRCFPGSPDGSVTSRLAHAVFEQIVRPCDYGIDFHNAAIRRTNYPNVRADLR
                     VAEVKRLARAFGSEIIVDAKGPDGSLRKTAVAAGVATIILEAGEIWKNEPGVVEVGVR
                     GILNVLKNLEMIEGKPEPPVFQITVGETTWVRAERGGFLSFHARPGEIVAAGQALATN
                     NSIFGRERQTLVSPVHGIVLGMTTMPAVKPGEPVYHIAILTERRYEEVKRRFEASPVD
                     ALYRRVQRDLATNIVLQERASNGACEGAA"
     gene            5672..6457
                     /gene="pssA"
                     /locus_tag="PLS90_13330"
     CDS             5672..6457
                     /gene="pssA"
                     /locus_tag="PLS90_13330"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPK03427.1"
                     /translation="MRKVFILPNLFTASSLFSGMLGIFEVFQGHLAAACGYILLSALL
                     DVFDGLVARLTKTQSAFGLNFDSLSDVVAFGAAPGLMAFSVLSAFYPRLAAPVASLFT
                     VCGALRLARFNVQATREESKAFLGLPIPGAALAAISLIWLVETKPELRPWLPLDKIYP
                     PAMVGLAYLMVSKVPYVGFKSLNLADRQPFEILVAIVVVITLLFMVKEHLLLVGASFI
                     WLYVLGGGFFALMRPIRRRRYRAYRQAMRARLRTSEAPREHES"
BASE COUNT         1017 a         2401 c         2048 g         1126 t
ORIGIN      
        1 cctctctcaa tcctcttatg tcctttgcgt cccttctgtc ccttcctttt tgcccgccgc
       61 cggaataagc tcttgatttt tcggcggtcg catcgtgtga tgtctatttc gtgcgcgttg
      121 acccggcgcg cgcgcaggat cgatgagcgt ccgcccgcgg ccgtccatct ccgagtcacc
      181 tttccaccga gaccaccatg gccaaagccc agcgtccgac cgccaatgaa ttgcagcgcg
      241 acgaagtcgc cgagagcctg caggaaacgc tgctcaccct tcagcgcaac cgcaagaaac
      301 tgatcgccgc cgctatcgtc ctgatcctcg gcgtcatcat cttcaccgcc gtgcgcagcc
      361 atcgcaacag cgtgatcatc gaatcgaacc gcctgatgtc ggacgcgctc gaaggctacg
      421 gtcggctgat ccaggccacc gatgaaaccg atcgccagcg cgaggtcgac tacacgctcg
      481 ccgcgctcga ccagttgcat gattcccacg gcgatacccc gctcggccat gaagccctct
      541 tcctgaaggg aaaaatccac tacgaactgg gcaactggga ggaggcccag cgcgcctacc
      601 agcagtacat cgatgaggcg cgcaccgccg aacagcgcgc ccagggacgc atcgcgctgg
      661 cctatgccca cgaaaacgct tccttttccc agcctaacga acaggcccgc cgcagcgaac
      721 tcgacgccgc cctgctcaat ttcgagcagg ctcaggatgc cgcccccgac cattcctacc
      781 tgacctatta tgccatgctc ggcgaagccc gttattacga gctgacgggc gacaacgagc
      841 gcgccatcgc cgtcctcgag cggatcgtca acgaacggcc ggcgcccgtc gcccagaccg
      901 actccgccga cgaagccgat tcgcccctcg acaccgaact gagcgaacaa ctccgcacgg
      961 cggtccgcga gcaggaatcc caggcgagtt tccaggccac cgccaaactg cgtctggagc
     1021 gcctgcgtgc ccgcgccggc ctcgaagccc ccgcgccggt caccgtcgtc gagacggccg
     1081 gcggcgcgcc ggcggccgac gccgatgccg cggcccccgg cgcaacccag gccgaaccgg
     1141 atgccgccga cggcgtctcg acgaccgaac ccgccccgcc cgccgcgtcc caagcggccc
     1201 cctgaaccat gttcaccggc atcattgagt ccgtcggggt catccgcgag cgtttgcaga
     1261 ccggcggcgg cctgcggctg gtggttcagc ccgatcggcc cctggatgcc ttgcgcgccg
     1321 gcgaatcgat tgcggtcaac ggcgtctgcc tcaccgccga agcctcttcc gctcccgacc
     1381 gtctctcgtt ctttctctcg cccgagaccc gccgccgctc cacgctcggc gacctccccc
     1441 tgggcgccct cgtcaatctg gagcgcgccc tgcgcgcgga agatcgcctc ggcggccacc
     1501 tcgtgctggg gcatgtcgac gccgtcggcc gcatccggcg gttcgatccc cagggcgaat
     1561 cgtggctgct ggaagtcggc tgcccgcgcg aactggagcc gtttctcgcc ccgaagggct
     1621 cgatcgccct cgacggcatc agcctgacca tggtcgagac cggcgaggac tggttcaccg
     1681 tcgccgtgat cccccacacg gcgcaggcga caacgctgcg gcgcgcggtg gccggcgacg
     1741 ccgtcaatct cgaagtcgat ctgctggcgc gctatgttgt ccgcgccctg gcgacgctgc
     1801 gcgccaggga gtcgggcctg agtctccaac tgctcaacaa agcggggttt ctgccgagcg
     1861 cgtgacgcag tccaacccgg ctcgacattc ccccttccgg caacaccccc ggctgagtga
     1921 ggcatgatgg ccaaggcggc cgacaccgct cccgcgcccc ccgatccgcg ccgcgcctgc
     1981 acgctggacc aggtcctcgg ccaccaggcc gtccgccgct acctgcgcca ggcctggcgc
     2041 cagcggcgtc tgccccaggc catcctgctg agcgggcccg agggcatcgg caaatcaacc
     2101 ctggcctggg cgctcgcgcg cgaaatcgcc gccgacggcc gtgatcccgc caccgacccc
     2161 aaatcgctca aggtcgtgcg cggcgtccac cccgacatgt ttgaaatcag cggcaagagc
     2221 accgtctcgc ggagcatcgt cgtggatgag atccggcggc tggaggatcg cgccatgacg
     2281 gccccgctcg aggccccgcg caaggtcttc gtcatcgacc cggccgaccg catgaatacg
     2341 gcggcggcca atgccctgct caagatcctc gaggagcccc acgggcgcct cgccttcgtc
     2401 ctggtcacca gtgtcccgca ccggctgctc gacacgatcc gctcgcgctg cgccgagctg
     2461 cgcctcgaac cggtcgccat cgacgaactg gcggcgtggc tgcgcgcgca ccgcccgctc
     2521 gacgagccgc gcgcgcgcct gcttgccgcc ctcgccgagg gccggcccgg tcgcgcgctg
     2581 caatgggccg gcagccgctg cctcgacgcc cgccgccaga ccatcgacgc gctgctgctg
     2641 ttccataccc acggctttgc ggccgtcttt catgtcgccg accggctggc tggcgccggc
     2701 gactcgctcg ccgaggggat cgccgtcgcc gttaccctcc tgcgcgacgc gctcgtcctc
     2761 gccatgcgcg gcgacggcgc cctcaactcc gatctcgtcg acaacctccg gcgtctgagc
     2821 gaggcctttc cgccccccgg cctcctcgcc gccgccgccc gcctggaggc cgccgccgcc
     2881 gaagccccct atttctatac cccgcaggcc aaggcccatt tcatggaatg cctggccacc
     2941 gacgtcggca aactcctgct gaccgccccc ggttgaacgt agactcaatc cgcccccgcc
     3001 cgctcaccac cctgacccaa acaactccat ctgccccacg accggacggc gaaacgcgtc
     3061 acaactgagc gcgggcggat caccgagccc cgcccgccga tgcgccaccc gaaacagcgt
     3121 ctcgatttga tcggcccacg acccttcccc ccggccgcgc gtcgcgaacc ggctgtcgta
     3181 cagtttccca ccgcgcatcg cgcgcagccg ccccagcacc ttgtcgcgct tgagcggttc
     3241 attccgctcc agccagtcgc tgaacaactc cttcagcccg tggggcaggc gcagcaggat
     3301 gtatcgcgcc cagcccgccc ccgcgtcggc caccgccttc acgatcgccg gcagttcatg
     3361 gtcggtcagc ccggggatca ccggcgcaat cagcaccccc accggcacgc cggcctcccg
     3421 cagtcggcaa atcgcctcga gccgcgcccg cggcgtcgac gtgcgcggtt ccagccgtcg
     3481 cgccagctcg gcgtccagcg tcgtaatcgt gatattcaca ttcgcgcatc cccggcgcgc
     3541 caactccgcc agcaggtcca ggtcgcgcgt gatctgggca ttcttcgtga tcatcaccac
     3601 cgggttcagg cattcggcca gcaccgccag gcagcgccgt gtgagctgta acctgcgttc
     3661 gatcggctga tacggatcag tcacgccgct cagcgccagc gcctgcggct tccacctggg
     3721 cgaggcgagc gcttcccgca gcagccgcgg cgcatcctcc ttgacgagga tccgcgtctc
     3781 gaaatcaagt cccgccgaaa aaccgagata ctcgtgcgtc ggccgcgcgt aacaataact
     3841 gcagccatgc tcgcacccac ggtacggatt gagactggcg ttgaagccga catccggact
     3901 gtcattgtaa gaaataatgg atttggaagc gtcgcgcaga aacagcgtct cgggcgccag
     3961 ccgctcctcc ggcgcaaggt cctcgtcaaa cggctccagc tcgacccgtt cgaagcgatt
     4021 gcgcggatta cccgcggccc cgcgtccgcg cggcgcttgt cgtgaacttt ccgggcgcgg
     4081 aacgtgttgg ggatcctggc tcatcccctc attctaccac aaacccctcc gcgtctccgc
     4141 ggctcggcat gagcaaatct ttccttcacg ccgccccttc acaggccccg ttcgacgccc
     4201 gttcctgcag gacaatattc gtcgccaggt cgcgctgtac gcgccggtag agcgcgtcga
     4261 cgggactcgc ctcaaaccgg cgcttcactt cctcgtaacg ccgctcggtc aggattgcga
     4321 tatgatagac cggctcgccc ggcttgaccg ccggcatcgt cgtcatcccc agcacgatgc
     4381 cgtggaccgg cgagaccagc gtctggcgct cgcggccgaa gatgctgttg ttcgtggcca
     4441 gcgcctgccc cgcggcgacg atttcgcccg gccgcgcatg gaaactcaga aacccgcccc
     4501 gttcggcgcg aacccacgtc gtttcgccga ccgtgatctg aaacaccggc ggttcgggct
     4561 tgccctcgat catttccaga ttcttcagca cgttcaggat gccgcgcacg ccgacttcga
     4621 ccacccccgg ctcgttcttc cagatctccc ccgcctccag gataattgtc gccacgccgg
     4681 ccgcgaccgc cgtcttgcgc agcgatccgt ccgggccctt cgcgtccacg atgatctcgc
     4741 tgccgaacgc ccgcgcgagc cgtttgacct ccgccacgcg caggtcggcc cgcacgttgg
     4801 ggtagttcgt ccgccggatc gccgcgttgt gaaaatcgat cccgtagtcg cacggccgga
     4861 caatctgttc gaacacggcg tgcgccagcc ggctcgtcac gctgccgtcg ggcgagcccg
     4921 gaaagcagcg gttcaggtcc cgccggtcgg gcaggtagcg cgagtgatgc tccagaccgt
     4981 agacattgac caccgggacg cagacgatcg ctccccgcag cagttccggc ggcttgtcat
     5041 acagcagatc acggatgatc ccgacgccgc tgagctcatc cccgtggatc gcgcccgtga
     5101 tgaacagccg cgggccgtcg gccggcgcgc ggatcacgcg gaccggcacc cccaccggcg
     5161 tgcccagata cgattcgctg aacggaagtt gcaggtcgcg gacttcgccg ggcgggatct
     5221 tgtggccggc gatgatcagg tccttgcgac gcgccatgct ggctctcggg atcaggttct
     5281 tgtaatttta cccgttaacg caaccccggt ccagatcgaa cccgatgccc gcttcggttc
     5341 tcatctctcg cctccggctc tcgccgacat ccttgcccgt cacagccgcg agagccggcg
     5401 ccatccggcg ttttaggggc cccctcgggg ctctgatgat atttgcactg ggccgtggct
     5461 ggccgaggtg cgggctcggg aggcaggagg gtaagggaaa ttcggaatct gggacccgat
     5521 cgaaggctca gatctcggtg acggaaacgt tgaacggctg gatgatggcg ggaggtggcg
     5581 gggacgagtg gtcctgattt cgctttcggg gcatcgaggg atctgattca atcggaaatc
     5641 agtctggccg ggtcccgtcg ggcgggaatc gatgcgcaaa gtttttatcc ttcccaatct
     5701 ctttaccgca agcagcctgt tcagcggcat gctggggatc ttcgaggtgt ttcagggcca
     5761 tctggccgcc gcctgcggct acattctgct gtcggctttg ctggacgtgt tcgatgggct
     5821 ggttgcgcgg ctgacaaaga cgcagagcgc ctttgggctg aatttcgatt cgttgtcgga
     5881 cgtggtggcg ttcggggcgg cgcccggcct gatggccttt tcggtgctct cggcgttcta
     5941 tccgcggctg gcggcgccgg tggccagtct gttcacggtg tgcggggcgt tgcgcctggc
     6001 gcggttcaac gtgcaggcaa cgcgcgagga gagcaaggcg tttctggggt tgccgattcc
     6061 cggggcggcg ctggcggcga tctcgctcat ctggctggtg gagacgaagc cggagctgcg
     6121 gccgtggttg cccctggaca agatctaccc gccggcgatg gtgggcctgg cctatctgat
     6181 ggtgagcaag gtgccgtatg tcgggttcaa gagcttgaac ctggccgacc gccagccgtt
     6241 tgagattctg gtcgcgatcg tcgtggtcat cacgctgctg ttcatggtga aggagcacct
     6301 gctgctggtg ggggcctcgt tcatctggct ttacgtgctg ggcggggggt tcttcgcgct
     6361 gatgcggccg atccggcggc ggcgctaccg ggcctaccgc caggcgatgc gagcgcggtt
     6421 gcggacgagc gaggcgccgc gcgaacacga gtcgtgagcg aggggggaac gccgcgccgg
     6481 ggcggggcgg tgtcggcatc gagaagactg gcctggcggc ggttatgcgc ggtggggcgg
     6541 acttcgccga cggcgtttgt gttggagccg gcggagggca atccgccggt tg
//
LOCUS       DAOPCO010000224         6570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10371_length_6570_cov_3.989409, whole genome shotgun sequence.
ACCESSION   DAOPCO010000224 DAOPCO010000000
VERSION     DAOPCO010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10371_length_6570_cov_3.989409"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLS90_13335"
     CDS             complement(<1..235)
                     /locus_tag="PLS90_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03428.1"
                     /translation="MSILITDPPQDALASTNSALDYCDAPTHDEAIRQIDEWARRHGF
                     ARVGERWLAVVSREDIGTCFKGVCYRLSEEDRAG"
     gene            complement(320..1957)
                     /gene="groL"
                     /locus_tag="PLS90_13340"
     CDS             complement(320..1957)
                     /gene="groL"
                     /locus_tag="PLS90_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887378.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPK03429.1"
                     /translation="MAKQMKYSEDARAAVLRGVDKLADAVKVTLGPKGRNVVLDKKFG
                     APTVTKDGVTVAKEIELEDPYENMGAQMVREVASKTSDVAGDGTTTATALAQAIFREG
                     AKNVTAGANPMAIKRGIDRAVEAVVADLAKRSIKTKDHSQIAQVATISANSDKVIGEL
                     IADAMDKVGKDGVITVEENKSIETVLDVVEGMQFDKGYLSPYFCTDMEKMTATFEDPF
                     ILIHDKKISSMKDLLPVLERVLQQGRPLVIIAEDIEGEALATLVVNKLRGTLNVAAVK
                     APGFGDRRKAMLEDIAILTGGKVISEDAGFKLENTQIGDLGRAARVLVEKENTTIIEG
                     RGDKKAIEGRIAQIKHQIEETTSDYDREKLQERLAKLAGGVAVIKVGASTEIEMKEKK
                     ARVEDALHATRAAVEEGIIAGGGTALIRSIPVLEGIKAEGDEAVGINVVRKALEAPLR
                     FIAQNAGAEGSIVVERVKNEKGNVGFDAAEGRYTDLVEAGIIDPTKVTRTALQNAASV
                     AGLLITTECMITEIKEKETHSHGHGGAPDMGGMGGMM"
     gene            complement(2034..2324)
                     /gene="groES"
                     /locus_tag="PLS90_13345"
     CDS             complement(2034..2324)
                     /gene="groES"
                     /locus_tag="PLS90_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPK03430.1"
                     /translation="MALKPLGDRVLVERIEEDVQKSAGGIIIPDTAKEKPQRGKIVAV
                     GDGKKDDDGKRIALDVAAGDVVIFGKYAGSEVKIDGKEYLIMHESDIMAKEA"
     gene            2935..4056
                     /locus_tag="PLS90_13350"
     CDS             2935..4056
                     /locus_tag="PLS90_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03431.1"
                     /translation="MALRPLGFVCAVLSATLTLPLGAQPPQPQTDSNLNPQVVVVTSD
                     GLMTLYSFANGTFAQVAQQQTDKSFIDVEIEDMDGDDIMDIATIQLNADSEKEAMVWG
                     YNPAGHTFVQKAVGPLDIDGDLVGLAVPTVTPNTIVVLFHVAGDEDHIRVFEVSGGDI
                     ATHPIDSGSYPVTNGGVPNNLYDDIAGGDVVTPDEATVGVDDVLVVHRGRPALLVNEL
                     LPRGLVASRFRHDFLDPVAIECLLPDDPPAAAGGGDLYALLAGDLRMIIMHTNAPMLQ
                     GAIYPDTDLGADGLTGIDCAAANLGGTADALAVLYAGDSPTAYRALRAVQPTPPATID
                     WLGDAVRVADTAVACDGARTGLLPLVPTPSQARHWTFQN"
     gene            4606..5130
                     /locus_tag="PLS90_13355"
     CDS             4606..5130
                     /locus_tag="PLS90_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPK03432.1"
                     /translation="MALAAAHSIGLTQRQAIDQLINDHRGRPGALLTVLERIQEAQPR
                     KFLPAEVLRYVAAAMRIPLSRVFSVVTFYAFFNLTPQGDNTVCICRGTACHTRGSRDL
                     LERLKLELGLTDTGPGEGSADKVSLTTPDSRFTVRTVACFGQCALAPVVEINRNIHGH
                     MNEREMHKAIQALR"
     gene            5146..>6570
                     /locus_tag="PLS90_13360"
     CDS             5146..>6570
                     /locus_tag="PLS90_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J/K"
                     /protein_id="HPK03433.1"
                     /translation="MRIEDFSTFNQVKESGLARLQPARPRLAVGMGTCGSGNGAERVY
                     LALREQIDQRGVDADLRRTGCFGFCAQEPLVNVWIPGRPLVILKRVDPRDVPRILDAL
                     LNDDVPRDLALCRIEDWDHITSRLQYGRGWNDIPLWNEVPFFKGQKKLVLRDCGIIDP
                     EEIEEYIAIGGYQALYKVLIDANPDAVIEQLKASKLRGRGGAGYLTGTKWEFLKRAAA
                     DRKYIICNADEGDPGAYMNRNEIESDPHALIEGMAIGGYVMGAAEGILYVRAEYPLAV
                     RRLHRAIEQAREYGLLGRNILGRGFDFDLHLVEGAGAFVCGEETALIASLEGRAGRPR
                     PRPPFPAQQGLWGKPTNINNVETWYNVPVIVLRGPAWFSEIGSPKSAGTKVFSLVGKV
                     RNTGLVEMPLGTPLKTFVYDVGEGTGNGKAVKAVQTGGPSGGCIPQEMFDTPVDYESL
                     AQIGSIMGSGGMIVMDEDNCMVDVA"
BASE COUNT         1052 a         2497 c         1809 g         1212 t
ORIGIN      
        1 cacccgcccg atcctcctcg gacaagcggt aacatacccc cttgaagcat gttccgatgt
       61 cctctctgct caccacggcc agccagcgtt ccccgacgcg ggcaaaccca tggcgacgcg
      121 cccattcatc aatttgccgg atcgcctcat catgggtcgg tgcgtcgcaa taatccagcg
      181 ccgagttcgt gctggccaat gcgtcttgcg ggggatcggt aatcaggatc gacatggcga
      241 tggttcccga atgtgagatg agagagcggc ccttggcgcc gccctctcat cccaaattac
      301 acgaaggccg gacggcggat tacatcatcc cgcccatccc gcccatgtcc ggagccccgc
      361 cgtggccatg gctgtgggtc tccttctcct tgatctcggt gatcatgcac tcggtcgtga
      421 tcagcagacc ggccaccgac gcggcgttct gcagcgccgt gcgcgtcacc ttcgtcgggt
      481 cgatgatccc cgcctccacc aggtcggtgt aacgaccctc ggcggcgtcg aacccgacgt
      541 tgcccttctc attcttcacc cgctcgacga cgatcgagcc ctcggctccc gcgttctgcg
      601 cgatgaaccg cagcggggct tccagcgcct tgcgcaccac gttgatcccc accgcctcgt
      661 cgccttcggc cttgatgcct tccagcaccg ggatcgagcg gatcagcgcg gtcccgccgc
      721 cggcgatgat cccttcttcc accgccgcgc gcgtcgcgtg cagcgcgtcc tcgacccgcg
      781 ccttcttctc cttcatttcg atctcggtcg acgcgccgac cttgatcacc gccacgccgc
      841 cggccagctt cgccagccgc tcctgcagct tctcgcggtc gtagtccgac gtcgtctcct
      901 cgatctggtg cttgatctgc gcgatccggc cctcgatcgc cttcttgtcc ccgcggcctt
      961 cgatgatcgt cgtgttctcc ttctcgacca gcacccgcgc ggcccggccc agatcgccga
     1021 tctgcgtgtt ctccagcttg aaccccgcgt cttcgctgat caccttcccg ccggtcagga
     1081 tcgcgatgtc ctcgagcatc gccttgcgcc gatcgccgaa gcccggcgcc ttgaccgccg
     1141 ccacgttcag cgtcccgcgc agcttgttca ccaccagcgt cgccagcgcc tcgccctcga
     1201 tgtcctcggc gatgatcacc agcggacggc cctgctgcaa gacccgctcc agcaccggca
     1261 acagatcctt catcgagctg atcttcttgt cgtggatcag gatgaacgga tcctcgaacg
     1321 tcgccgtcat cttctccatg tcggtgcaga aatacggcga caggtagccc ttgtcgaact
     1381 gcatcccttc gaccacgtcc aggacggtct cgatcgactt gttctcctcg accgtgatca
     1441 ccccatcctt gccgaccttg tccatcgcgt cggcgatcag ctcgccgatc accttgtcgc
     1501 tgttggccga gatcgtcgcc acctgcgcga tctgcgagtg atccttcgtc ttgatcgagc
     1561 gcttggccag atcggcgacc accgcctcaa cggcccggtc gatcccgcgc ttgatcgcca
     1621 tcgggttggc cccggcggtc acgttcttgg ccccctcgcg gaagatcgcc tgggccagcg
     1681 ccgtcgccgt cgtcgtcccg tcgcccgcca catcggacgt cttcgaggcg acctcgcgga
     1741 ccatctgcgc gcccatgttc tcatacggat cttccagctc gatttccttg gccacggtca
     1801 cgccgtcctt cgtcacggtc ggcgcgccga atttcttgtc caggaccacg ttgcggccct
     1861 tcggccccag cgtcaccttg acggcgtccg ccagcttgtc cacgcctcgc agcacggcgg
     1921 cgcgtgcgtc ttcgctgtat ttcatctgct tagccatgtt ctgcttcttc ctcctggctc
     1981 attcggggat gttggtctgg attccggtct tgctcgaccg gcacggggga gcctcaggct
     2041 tccttcgcca tgatgtcgct ttcatgcatg atcaggtatt ctttgccgtc gatcttcact
     2101 tcgctgccgg catacttgcc gaaaatcacc acgtcgccgg cggccacatc cagcgcgatg
     2161 cgcttgccgt cgtcatcctt cttgccgtcg cccacggcga cgatcttgcc gcgctgaggc
     2221 ttctccttcg cggtgtcggg gatgatgatg ccgcccgcgg acttctgcac gtcttcttca
     2281 atccgctcaa cgaggacgcg atcgcccagg ggtttgagag ccattgtcaa tgaaccctcc
     2341 ctttctatgg tttcagttgg gtaactgcaa gaatcatggt ccgggcggag gacggagcgc
     2401 cgccacggcc ttttcagacc cgacctactc cagcaatgcc tattccaccc ccgggaataa
     2461 atatccaagt cctttggttt caatcaacca ggcccttcat cgggcccgcg ccgaaccacc
     2521 gtaacccgtc gtgttgatgc attctgatac acatccccct ccgctccccc caatctgtgt
     2581 caaaacggac aagtcgccat tccccttcgc actgctcttg ggtatcggta tcgggatcgc
     2641 tctcggtatc ggcatcgcga ccgttcccga ccgcgcctcc gcgccctccc aaaatcagtt
     2701 gacacgccgg tcatctattg tgatatcgtt ttctcagcaa gggatatcgt ttacgcatcc
     2761 ggctccgcgc ccggctccat tctggcccgc tggtccgctc cccccgcgcg atccacccgc
     2821 tccgacgggc cgtgcgccga cctcgccaag ccaagctgga gcggacgacc tcggcccccc
     2881 acaggggcga gattcccaat catcccgcgc tccgcacatc aggaggcccg tcccatggct
     2941 ttgcgacccc tcggattcgt ctgcgccgtt ctgagcgcga ccctcacgct tcccctcggg
     3001 gcgcagcccc cccaacccca gacggattcc aacctcaacc cccaggtggt cgttgtcacc
     3061 agcgacgggc tgatgacgct ctattccttt gccaacggaa ccttcgccca ggtcgcccag
     3121 cagcagaccg acaaatcctt catcgacgtt gaaatcgagg acatggatgg cgacgacatc
     3181 atggacatcg ctaccattca gctcaacgcc gattccgaga aggaagccat ggtctggggc
     3241 tacaaccccg ccggccacac ctttgttcaa aaagccgtcg gcccgctcga tatcgacggc
     3301 gacctcgtcg gcctggccgt gcccaccgtc acccccaaca cgatcgtcgt cctcttccac
     3361 gtcgcgggcg acgaggacca tatccgcgtc ttcgaggtca gcggcggcga catcgccacg
     3421 catccgattg attccggctc ctacccggtg accaacggcg gcgtgcccaa taacctctac
     3481 gacgacatcg ccggcggcga cgtcgtcacg cccgacgaag ccaccgtggg cgtcgacgac
     3541 gtcctcgtgg tgcatcgcgg ccggcccgcc ctcctggtca acgagctgct cccccgcggc
     3601 ctggtggcca gccgcttccg ccacgatttc ctcgatcccg tggcgatcga gtgcctcctg
     3661 cccgatgacc ctcccgcggc ggccggcggc ggcgacctct acgcgctcct cgccggcgac
     3721 ctgcgcatga tcatcatgca caccaacgcc cccatgctgc agggcgcgat ctatcccgac
     3781 accgacctcg gcgccgacgg cctgaccggc atcgattgcg ccgcggccaa cctcggcggc
     3841 acggccgatg ccctcgccgt cctctacgcc ggcgactctc ccaccgccta ccgcgccctg
     3901 cgggcggttc agcccacgcc ccccgcgacg atcgattggc tgggcgacgc cgtccgcgtc
     3961 gccgacaccg ccgtcgcctg cgacggcgcc cgcaccggcc tgctccccct cgtccccacc
     4021 cccagccagg cccggcactg gaccttccag aattagcgcc ggttccctca ctcaaagttc
     4081 ggctctcggc cctcgtcctc ggctctcgaa gatccgccgt ttctgccatt cgctggaaag
     4141 atcccaaatc cagtgctgtc ggttccgact gccgaaccgg ctaagtctgt gaagcagacg
     4201 accggagtaa agcgcagggg gacggccgcg ggccgaaacc ctgcgtcgtg gctccccatg
     4261 agtcaagccc cgggcagggg cggccggata ggcttgcatc atacggctga cggccgcccg
     4321 aagcaggaaa ttaggctgcg aaagtctttt tttgtttgac atttcgcccc cggttgcccc
     4381 tatttgaatc cgttgggaag tcgcacacat acacgcaacg cgcgggtcgc ccaaaagcag
     4441 aacggattgc cggccgcccc agaaacccag atgacgcagt cccccatgat gcaggttttc
     4501 ttccaccctc gtcatttgag cccgccggat cgtttaacgg ccgcgatctg tgaaacattt
     4561 cacaagcggg cccccgcccc gaccctgcgc cggaggacct aacccatggc tctcgccgcc
     4621 gcccattcca tcggtctgac ccaacgccag gcgatcgatc agctcatcaa cgatcaccgc
     4681 ggccgccccg gcgcgctgct gaccgtcctg gagcggatcc aggaggccca gccccgcaag
     4741 ttcctccccg ctgaggtcct gcgctacgtc gccgccgcca tgcggattcc cctctcgcgc
     4801 gtcttcagcg tcgtcacctt ctacgccttc ttcaacctga ccccgcaggg cgacaacacc
     4861 gtctgcatct gccgcggcac tgcctgccac acccgcggct cgcgcgatct gctcgagcgc
     4921 ctcaagctcg agctcggcct gaccgacacc ggcccgggcg aggggagcgc cgacaaggtt
     4981 tcgctcacga cccccgacag ccgcttcacc gtccgcaccg tcgcctgctt cggccagtgc
     5041 gccctggcgc ccgtcgtcga gatcaaccgc aacatccacg gccacatgaa cgaacgcgaa
     5101 atgcacaagg ccatccaggc tctccggtag ggaaggaccg cgccgatgcg tatcgaagat
     5161 ttcagcacgt tcaaccaggt taaggaatcg ggtctggccc ggctccagcc ggcgcggccg
     5221 cgcctcgccg tcggcatggg cacctgcggt tcgggcaacg gcgccgagcg cgtctacctg
     5281 gcgctgcgcg agcagatcga tcagcgcggc gtcgatgccg acctgcgccg caccggctgc
     5341 ttcggtttct gcgcccagga gccattggtc aacgtctgga tccccggccg cccgctggtc
     5401 atcctcaaac gggtcgatcc gcgcgacgtc ccccgcatcc tcgacgcgct gctcaacgac
     5461 gatgtcccgc gcgacctcgc cctctgccgc atcgaggact gggatcacat caccagccgc
     5521 ctccagtacg gccgcggctg gaacgacatc cccctctgga acgaggtccc cttcttcaag
     5581 ggtcagaaaa aactggtcct gcgcgactgc ggcatcatcg accccgagga gatcgaggaa
     5641 tacatcgcca tcggcggcta ccaggcgctc tacaaggtcc tgatcgacgc caacccggat
     5701 gccgtcatcg agcagctcaa ggcctccaag ctccgcggcc gcggcggcgc cggctacctg
     5761 accggcacca agtgggaatt cctcaagcgc gccgcggccg accgcaaata catcatctgc
     5821 aacgccgatg agggcgaccc cggcgcctac atgaaccgca acgagatcga gagcgatccc
     5881 cacgccctga tcgagggcat ggcgatcggc ggttacgtga tgggcgccgc cgagggcatc
     5941 ctctacgtcc gcgccgagta ccccctcgct gtccgccggc tccacagggc catcgagcag
     6001 gcccgcgaat acggcctgct cggccgcaac atcctcggcc gcggcttcga cttcgatctg
     6061 cacctggtcg agggcgccgg cgccttcgtt tgcggcgagg agaccgccct catcgcctcg
     6121 ctcgagggcc gcgccggccg cccgcgcccc cggcccccgt tccccgccca gcagggtctg
     6181 tggggcaagc ccaccaacat caacaacgtc gaaacctggt acaacgtccc cgtcatcgtc
     6241 ctgcgcggcc ccgcttggtt ctccgagatc ggcagcccca agagcgccgg caccaaggtc
     6301 ttctccctcg tcggcaaggt ccgcaacacc ggcctcgtcg agatgccgct gggcaccccg
     6361 ctcaagactt tcgtctacga cgtcggcgag gggaccggca acggcaaggc ggtcaaggcc
     6421 gtccagaccg gcggcccctc cggcggctgc atcccccagg agatgttcga cacgccagtc
     6481 gattatgaat cgctcgccca gatcggctcg atcatgggat cgggcggcat gatcgtcatg
     6541 gacgaagaca actgcatggt cgacgtcgcc
//
LOCUS       DAOPCO010000225         6539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10417_length_6539_cov_4.667027, whole genome shotgun sequence.
ACCESSION   DAOPCO010000225 DAOPCO010000000
VERSION     DAOPCO010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10417_length_6539_cov_4.667027"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            825..1313
                     /locus_tag="PLS90_13365"
     CDS             825..1313
                     /locus_tag="PLS90_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03434.1"
                     /translation="MGAMDMTRILVAVDNFSTEAYLKDLFKHYGIRANWAWGWNGTVL
                     HLERGAYDALLLDDLLIPPDVRSPARWLRARGHEIPVITMVEHPALRRAREAGEIAIG
                     KPVSPIQLMEALAAAVGDAEGRQDQAGEMRRRPASRKSRKFELTRRSRQRIRTLERSV
                     AS"
     gene            1310..1600
                     /gene="groES"
                     /locus_tag="PLS90_13370"
     CDS             1310..1600
                     /gene="groES"
                     /locus_tag="PLS90_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPK03435.1"
                     /translation="MNIKPLGDRVLVQRIEEEVQKSAGGIIIPDTAKEKPQMGKVVAV
                     GDGRRDDQGKRVPLDISAGDEVFFGKYAGNEIKVNGTEYLIMREDDVIAKRV"
     gene            1639..3273
                     /gene="groL"
                     /locus_tag="PLS90_13375"
     CDS             1639..3273
                     /gene="groL"
                     /locus_tag="PLS90_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014449688.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPK03436.1"
                     /translation="MPAKQMLYEERARAAILRGVNALADAVKVTMGPKGRNVVLDKKF
                     GSPTITKDGVTVAKEIDLEDPFENMGAQMVRQVASKTSDVAGDGTTTATVLAQTIYAE
                     GLKNVTAGSNPMALKRGIDRAVEAVVEDLRKRSTETKEHSQIAQVARISANNDQVIGD
                     LIADAMEKVGKDGVITVEEAKSIETVLDLVEGMQFDKGYLSPYFCTDMEKMTATLEEP
                     YILIHDKKISSMKDLLPVLERVLQQGKPLLLIAEDVEGEALATLVVNKLRGTLNVAAV
                     KAPGFGDRRKAMLEDIAILTGGKVISEDAGFKLENTQIGDLGRAARVLVEKENTTIIE
                     GRGDKKAIEGRIAQIKHQIEETTSDYDREKLQERLAKLAGGVAVIKVGASTEIEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALVRSLGALDGVKAEGDEQIGVNIVRRALEAPL
                     RNIAINAGAEGTIVVEKVKSENGNMGYDAEHGTYTDMMQAGIIDPTKVVRSALQNAAS
                     VAGLLLTTECLVTEIKEKKSQKPLPGGGGDLDDYAD"
     gene            3436..5754
                     /locus_tag="PLS90_13380"
     CDS             3436..5754
                     /locus_tag="PLS90_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK03437.1"
                     /translation="MNHSVSTIAMIGTYVPRQCGIATFTKDLRDAVAEGNVETAVISL
                     DDRPEGYDYTEEVRFQIRANRLQDYRLAADMLNINQIDVAILQHEYGIFGGPAGRHVL
                     ELAAGLRMPLITTLHTVLREPSREQHFVISELARLSDRLVVMSELARNMLREVYQVAA
                     EKVAVIPHGIPDVPFVDPAFFKDQYGLEGRDVLLTFGLLSSGKGIEIAIQAMPRIVRE
                     HPETAYVVLGATHPHILKREGNAYLHSLQRLAARLGVAEHVVFHNRYVTLEELCGYMG
                     AADIYLSPYLNKAQIVSGTLAYAMGMGKAVVSTPYWYAEELLSEGRGRLVPFGDPEGL
                     ADAVNELLEDPVQRHTMRKRAYLEGRSMVWKEVGRGYVQLANEVIEERHREPRPIFSL
                     SSARSPLRGIPEINIDHLRILTDDTGILQHAVYTVPDRFHGYCTDDNARALIAALKYY
                     ALSQDSTALGLAGIYMGFLYHAYNAETKRFRNFMSYDRRWSEDVGSEDVHGRVVWALG
                     QTVALAPNEGVLSFGTRLFNVALESLPEFQSPRAWAFALIGILSYLDRFPGDAVARRI
                     RAELSGRLLQLFKNNATADWPWCEDVVTYDNAKLPHALIASGHWGQEEELLETGLGAL
                     EWLVNQQVTEDGRISIIGNQGWLERGGARARFDQQPIDAMALIEACAEAYRCTNRPVW
                     LARARQCFSWFLGNNETEAMLFDPATGGCRDGLHAHGPNLNQGAESTLAWLMSLIIIH
                     QLESTFGVVEEPGGEADAELREESLTSQGGAA"
     gene            6066..>6539
                     /locus_tag="PLS90_13385"
     CDS             6066..>6539
                     /locus_tag="PLS90_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPK03438.1"
                     /translation="MTLPFEVNTYVQVADIFERVARLSDDEVSQALAEVLRDFGDRHN
                     QLTHRLEEHYAIAARLVDLKEELSRERKLLLGAYFTMEYSLESTALFNPSIVPHPDQS
                     GAGPGEVRFVMSLRALGEGHLSSTVFQTGTIAADGSFRLDPPSGFSARTRVNPDRI"
BASE COUNT         1302 a         1646 c         2559 g         1032 t
ORIGIN      
        1 tcatcccgta aattcttttc ccccacgggc gggatgggaa cttgcgacga tgatgagcga
       61 tggctgttgg ttgaggcgtc acgcgtttca caaaaacaga agattattcg gagtcgggtg
      121 ggtctcacag gggcgtgccg ggtgtgggag aaaaccgttg aaacggttga gggatattat
      181 tgcgtcgggc caccggccga ggcccggtgc caatgacaga gcccccgagg gggccagcga
      241 taagcccgct ggcgcgggcg ctcaggatga aacggattcg agagccgagg acgagggacg
      301 agagccggga gccgagtttt ggggcggtcg gttgggggcg aggacgaaag agtgacggat
      361 tttggagtgg gggcgttcat gttgcaagaa gtgcgacgct gcaacgatga gaaatggttg
      421 cctgattctg tgttggtgaa gttcgccccc tacggggacg gggtttttgc gggtggcgtt
      481 cccaggttcc gctgcgctgc accaggggct attcaaatgg cgcccctgcg gggtgcgggc
      541 ggggggcgga atttggggga gggagctccg cggctcgcgt cggcgggtga ggcgatgcag
      601 ccgcggatgg aaaagacaat ctcacgcgga ggcgcagaga cgcggaggcg tggcggcgtg
      661 gagccgccat ctgccggcta ggatgccggc ggtcccaggc ggtggctgat gtgacgatgg
      721 cccgaattcg agagccgagg acgaagacgc gggacgaaat tttaataaga atgaaatgga
      781 gataagcgtc caaccggaaa agaacaagaa cgtgttgatg gagagtgggt gcgatggata
      841 tgacacgaat tcttgtcgcg gtcgataatt tttccaccga agcgtatctg aaggatctgt
      901 tcaagcatta tggcatccgg gcgaattggg cgtggggttg gaacgggacg gtgctgcatc
      961 tggagcgcgg cgcgtatgac gcgctgctgc tggatgattt attgattccg ccggacgtgc
     1021 ggagcccggc gcggtggctg cgcgcgcgcg gacatgaaat tccggtgatt acgatggttg
     1081 agcatccggc cctgcggcgc gcgcgggagg cgggggaaat tgcgatcggc aagccggtga
     1141 gcccgatcca actgatggag gcgctggcgg cggcggtggg ggacgccgag ggccgtcagg
     1201 accaagccgg ggaaatgcgc cggaggccgg cgtcgcgaaa aagcaggaag tttgagttaa
     1261 cgagaagatc gagacagcgc atcagaacac tggaaaggag cgttgcgtca tgaacatcaa
     1321 gcctctgggg gaccgggtac tggtccagcg gatagaggaa gaggtgcaga aatcggcggg
     1381 gggaatcatc attcccgata ccgcgaaaga gaagccgcag atgggcaagg tcgtcgcggt
     1441 gggcgacggc cggcgtgatg atcagggcaa gcgcgtgccg ctggacatca gcgccgggga
     1501 cgaagtgttc ttcgggaaat atgccggcaa tgaaatcaaa gtgaacggca cggaatatct
     1561 gatcatgcgc gaagacgacg tgatcgcgaa gcgagtctga ggacggaaag cgaagttcat
     1621 cgaggaggag aaaggaccat gccagccaag caaatgctgt acgaggaacg cgccagggcg
     1681 gcgatcctgc gaggggtgaa cgcgctggcc gatgcggtga aggtgacgat gggcccgaag
     1741 ggtcgcaacg tcgtcctgga taagaagttc gggtcgccga cgatcacgaa ggacggggta
     1801 accgtggcca aggagatcga tctggaggat ccgttcgaga acatgggtgc gcagatggtg
     1861 cgccaggtgg cgtcgaagac gagcgacgtc gccggggacg ggacaacgac ggcgacggtg
     1921 ctggcgcaga cgatctatgc cgaagggctg aagaacgtga cggcggggtc gaacccgatg
     1981 gcgctcaagc gggggatcga ccgggcggtc gaagcggttg tggaggacct gcgcaagcgg
     2041 tcgaccgaaa cgaaagaaca cagccagatc gcgcaggtgg cgcggatctc ggcgaacaac
     2101 gaccaggtga tcggcgacct gatcgccgac gcgatggaaa aggtgggcaa ggacggcgtg
     2161 atcaccgtcg aggaggcgaa gagcattgag acggtgctgg acctggtcga agggatgcag
     2221 ttcgacaagg ggtatctgtc gccgtatttc tgcaccgaca tggagaagat gacggcgacg
     2281 ctggaggagc cgtacatcct gatccacgac aagaagatca gctcgatgaa ggacctgctg
     2341 ccggtgctgg agcgggtgct gcagcagggc aagccgctgc tgctgatcgc cgaggacgtg
     2401 gaaggcgagg cgctggcgac gctggtggtg aacaagctgc gcgggacgct gaacgtggcg
     2461 gcggtcaagg cgccgggctt cggcgatcgg cgcaaggcga tgctcgagga catcgcgatc
     2521 ctgaccggcg ggaaggtgat cagcgaagac gcggggttca agctggagaa cacgcagatc
     2581 ggcgatctgg gccgggccgc gcgggtgctg gtcgagaagg agaacacgac gatcatcgaa
     2641 ggccgcgggg acaagaaggc gatcgagggc cggatcgcgc agatcaagca ccagatcgag
     2701 gagacgacgt cggactacga ccgcgagaag ctgcaggagc ggctggcgaa gctggccggc
     2761 ggcgtggcgg tgatcaaggt cggcgcgtcg accgagatcg aaatgaagga aaagaaggcg
     2821 cgcgtcgagg acgcgctgca cgcgacgcgc gcggcggtgg aagaagggat cgtcgccggc
     2881 gggggcgtgg cgctggtgcg cagcctgggt gcgctggacg gggtgaaggc cgagggggat
     2941 gagcagatcg gcgtgaacat cgtgcggcgg gcgctggagg cgccgttgcg caacatcgca
     3001 atcaatgccg gggccgaagg gacgatcgtc gtggagaagg tcaagagcga aaacggcaac
     3061 atgggctacg acgccgagca cgggacgtat accgacatga tgcaagcggg gatcatcgac
     3121 ccgacgaagg tggtgcgctc ggcgctgcag aacgcggcgt cggtggccgg gttgctgctg
     3181 acgaccgaat gtctggtgac ggagatcaag gagaagaaga gccagaagcc gctgccgggc
     3241 ggcggcgggg atctggacga ctacgccgat taaggggaca aaacgagccg ccgcgagggg
     3301 agatggccgc ggcggccgga cagggtcgac ggcgtagcgt cgggaccgcc cctgactgcg
     3361 cctccacgca gatcaggggc ggttttcatg aacgggaagt tccccgcccg aagttgagaa
     3421 aggagcgcgg agttcatgaa ccactcggta tcgacgattg cgatgatcgg cacgtatgtg
     3481 ccgcggcagt gcggcattgc cacgttcacg aaggatctgc gcgatgcggt ggccgaaggg
     3541 aatgtggaga cggcggtgat cagcctggac gaccgaccgg agggctatga ttacacggag
     3601 gaagtgcggt tccagatccg cgccaaccgg ctgcaggatt atcgcctggc ggccgacatg
     3661 ctgaacatca accagatcga cgtcgcgatt ctgcagcacg agtacgggat tttcgggggg
     3721 ccggcggggc ggcacgtgct ggaattggcg gcggggctgc ggatgccgct gatcacgacg
     3781 ctgcatacgg tgctgcgcga gccgagccgg gagcagcact ttgtcatcag cgagctggcg
     3841 cggctgagcg accggctggt ggtgatgagc gagctcgcgc gcaacatgct gcgggaggtg
     3901 tatcaggtgg cggccgagaa ggttgcggtc atcccgcacg ggattccgga cgtgccgttc
     3961 gtcgatcccg cgtttttcaa ggaccagtat ggcctggaag ggcgcgacgt gctgttgaca
     4021 ttcggactgc tgtcgtcggg caaggggatc gagatcgcga tccaggcgat gccgcggatc
     4081 gtgcgggagc atcccgagac ggcgtatgtc gtgctggggg cgacgcaccc gcacattctc
     4141 aaacgcgagg ggaacgcgta tctgcacagc ctgcagcggc tggcggcgcg gctgggggtg
     4201 gcggagcacg tcgtgttcca caatcgctat gtgacgctgg aagagctgtg cgggtacatg
     4261 ggcgcggccg acatttacct gagtccgtat ctgaacaagg cgcagatcgt gtcggggacg
     4321 ctggcctacg cgatggggat gggcaaggcg gtggtgtcga cgccgtactg gtatgcggag
     4381 gaactgctgt cggaggggcg cgggcggctg gtgccgttcg gcgatccgga ggggctggcc
     4441 gatgcggtca atgaactgct cgaagacccg gtccagcgcc atacgatgcg gaagcgggcc
     4501 tatctggaag ggcggtcgat ggtctggaag gaagtggggc gggggtatgt tcaactggcg
     4561 aacgaggtga tcgaggagcg gcaccgggag ccgcggccga tcttctcgct gtcgtctgcg
     4621 cgatcgccgc tgcgcggcat tccggagatc aacatcgatc acctgcggat tctgaccgac
     4681 gacacgggga tcctgcagca tgcagtctac acggtgcccg accggtttca cgggtactgc
     4741 acggacgaca acgcgcgggc gctgatcgcg gcgctgaagt attacgcgct gagccaggac
     4801 agcacggcgc tgggcctggc cggcatttat atgggattcc tgtaccatgc ctacaacgcc
     4861 gagacgaagc ggttccgcaa cttcatgagc tacgaccggc gctggtcgga ggacgtcggc
     4921 agcgaggacg tgcacggtcg ggtcgtgtgg gcgttggggc aaacggtggc gctggcgccg
     4981 aacgaggggg tgctgtcgtt cgggacgcgg ttgttcaacg ttgcgctgga atcgttgccc
     5041 gagtttcaat cgccgcgggc gtgggcgttt gcgctgatcg ggattctgtc gtatctggac
     5101 cggttcccgg gcgatgcggt ggcgcggcgg attcgagcgg aactttccgg ccggctgctt
     5161 cagctgttca agaacaacgc gacggcggac tggccgtggt gcgaggacgt ggtgacgtat
     5221 gacaacgcca agctgccgca cgcgctgatc gcgagcgggc attggggcca ggaggaggaa
     5281 ctgctggaga cggggctggg ggcgctggag tggctggtga accagcaggt gacggaggat
     5341 gggcggatct cgatcatcgg gaaccagggg tggcttgaac gcgggggggc gcgcgcgcgg
     5401 ttcgatcagc agccgatcga tgcgatggcg ctcatcgagg cctgcgccga ggcctaccgg
     5461 tgcacgaaca ggccggtgtg gctggcgcgg gcgcggcagt gcttcagttg gtttctgggc
     5521 aacaacgaaa cggaggcgat gctgttcgac ccggcgacgg gaggctgccg cgacgggctg
     5581 cacgcgcatg ggccgaatct gaaccagggg gcggagtcga cgctggcgtg gctgatgtcg
     5641 ctgatcatca tccatcagct tgagtcgacg tttggggttg tggaggagcc cgggggggag
     5701 gcggacgcgg aattgcggga ggaatcgctc acgtcgcagg gcggggcggc gtagcggcaa
     5761 ggaacgggga tgacggccaa cacgcaggga ggctcggaaa tacaggagga gtcatgtcgg
     5821 cgccggggca ggaacccgaa ggaaggaatc ccgcgccgag ccgccgagcc gccgaggtgt
     5881 gacggccagg cgggtgggcc tgggatagaa tgttaggttt tcgagagccg agacgaggga
     5941 cgatattgga gcgagggacg agtccaacgt ttggcgcgga gagcggtatg aacggcgagg
     6001 gatcgagggg gcggctgaag atcagctttc ttcccgtgca tctgaataat aatcgacggc
     6061 gggtggtgac gctgccattc gaggtgaaca cctacgttca ggtggcggac atcttcgagc
     6121 gggtcgcgcg cctgtcggat gacgaggtga gccaggcgct ggcggaggtg ctgcgcgatt
     6181 tcggcgaccg tcacaatcaa ctgacgcacc ggctggagga gcattatgcg atcgccgcgc
     6241 ggctggtgga cctgaaggag gaattgtcgc gcgagcggaa gctgctgctg ggcgcgtatt
     6301 tcacgatgga gtattcgctg gaatcgacgg cgctgttcaa tccgtcgatc gtgccgcatc
     6361 ccgaccagag cggggccggg ccgggggaag tgcggttcgt gatgagcctg cgcgcgctgg
     6421 gcgagggcca cctgtcgtcg acggtgtttc aaacggggac gatcgcagcg gacggatcat
     6481 tccggctgga tccgccgagc ggattttcgg cgcgcacccg cgtcaatccg gaccgcatt
//
LOCUS       DAOPCO010000226         6534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10432_length_6534_cov_5.252354, whole genome shotgun sequence.
ACCESSION   DAOPCO010000226 DAOPCO010000000
VERSION     DAOPCO010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_6534_cov_5.252354"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1254)
                     /locus_tag="PLS90_13390"
     CDS             complement(133..1254)
                     /locus_tag="PLS90_13390"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK03439.1"
                     /translation="MAVSFLDLSRQHEPLKKELLHAIEQVIDSSEYILGSAVARFEQN
                     FAQRLKASHCVGVNSGTDALLLTLHALGIQPGDQVICPAFGYVAMADVIPRMGAHIVF
                     ADINEHFTIDVEHVRQLMNENTRAVIAAHMFGRMADVETLAQMTAEQGVHLIEDCSHA
                     CGAEINGRPAGTWGTAGAFSFSPERNLGCIGDGGAIVTNNPELAQRLRLYRDHGAEAE
                     NRYVEIGYNSRLDAIQAAILDVKLGTLDEDNADREANAAFYQNHLSSEVFALPDPGPE
                     GSHVFNYYTIRSTMRDQLRSFLKDRMVDTRVYYPVPLHLQPCFEYLGYTEGHIPVAEQ
                     MAKEVLSLPVSPGLTRQELEEVAHTIDLFAQTYTTPTAG"
     gene            complement(1425..2594)
                     /locus_tag="PLS90_13395"
     CDS             complement(1425..2594)
                     /locus_tag="PLS90_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPK03440.1"
                     /translation="MNAPPAPTIFYDARNIRRGMTGVGRYTYNLLAGLAALEEAPPIR
                     AMFLPGAVEIARRDPALARVDVVAAMFGHEAHPQTDIWLRWGLSRRLPAGSVYHGPVF
                     IVPGGRQPFGRVVTVHDLFVFTHPQFFPLKFRAWMRWAIRRACRFSDRIISVSATVAE
                     QIVERGLAERARIRVVREAADATPMLWEHGGGEEMGVEEPAAGPLIVTVGTRDPHKNP
                     ATARRACIELARRWPAGRPPFEWWWIGGTGPLPDDSSESLRRQAEKAGFRAPGALPSA
                     VVRRILRRAAVLVSCSHTEGFNLTLVEAMRAGCAIVASDLPIHREVAGGTAQFFPATD
                     WAALAGILLRLLTDEEELAAWRRRSAERAGAFSWERTARETLAVYREIWRGRGGV"
     gene            complement(2596..3714)
                     /locus_tag="PLS90_13400"
     CDS             complement(2596..3714)
                     /locus_tag="PLS90_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPK03441.1"
                     /translation="MHDEFPILRDRVYLNHAAVAPIPARAGRALREFADEAVEGGGAL
                     WGRWAARLKAARAEAARLLDVQVDDVAFVHNTTHGLLCIANSLPWRPGDNIVGMAADF
                     PANVHPWRNLAQQGVALRAVEPGPDQRYRAEDFAALLDERTRLVALSLVHYASGCRLP
                     VERIAEICRRRGVRLCVDAIQAVGALPTRPVDLGCDYLVADGHKWMLGPEGLGILWVH
                     PDRIEEMNVCMTGWTGRVRYSQYEDYDQPLLPNARRFEEGSYNMAGAAALGQSLALLN
                     EIGIDEVWRRLDALTGRLREGLRSAGWTIVSPDGEGERSGITSAARAGVDVQALVNRL
                     EERRISVSPRGGRLRIAPHFYNTAGQLDELIAALRELA"
     gene            4176..5813
                     /locus_tag="PLS90_13405"
     CDS             4176..5813
                     /locus_tag="PLS90_13405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03442.1"
                     /translation="MPRSPARTGSAIRRLRPILLGLIVLCAFVLRVTFNTYQLPPYWD
                     YPNEIDKMVDVLALEELGFYHGYMQPSFLMNTMWLWYQPAQVVFPWVNATFPGFRPLE
                     GGMEGWLLWLGRVYMALFGAATVWLVYFVGRRIGSARLGLTAALLLAVTPIHVLGSRH
                     MKEDMPLAFFVTLALLFLLDVVRRGARRDYLRAGLAAGLAFSTKWIGISILAPFLLAG
                     LLAIPRTRRNWPAPGRRWLIPLILASFVAGFLLASPDYILAGRMNIIWEGPQKGMTKA
                     YDAHADGIAVDAYREAYVDYFRKGIWPGLTPALILLAGIGFGSLWRRRRSAALLLGFW
                     VVFFYILIESARARPYPHFERYLQPITPTLIVFAACGVEAARRGALRLLRQRRGFPPL
                     RRAVAPALLCLALAWPLADTIRYLEHIPRSTVLQAREWIMQHVPPGAIIYDDSYGPGF
                     PEGRYERRKYRSKDQLIVGAFPTEPCYVMYTSMGLGRFVEHPEANPGVARWVFYVLSK
                     GRLTKEWTPEFRSFYAESPLIKLYRFEPSPDPLVVEY"
     gene            complement(5896..>6534)
                     /locus_tag="PLS90_13410"
     CDS             complement(5896..>6534)
                     /locus_tag="PLS90_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03443.1"
                     /translation="EPRAADAALASLASRLEEVVVQTMESRIAALEAQVQTAIAAPGA
                     ASGAGAGRPAQTAAAMGSDFEIGAVPEAGIVPVVSSAELEAAAAEPVAPVEFELASAY
                     ALDPSVPEVLYEAAPVPEINGRLVSLDAAEGERPRAARPAPAGKPKHEAPASPTDGLR
                     VEGIFWAKTRPMALINGQIVEVGSRIGNAKVTAINPGSVTLEINGREHTILP"
BASE COUNT         1022 a         2481 c         1911 g         1120 t
ORIGIN      
        1 tatgcagacg ccccatcaat cctacagaaa aaacatgggc cgcggcggtc ccccggaagg
       61 gccgtcttct caataacaaa cggccgccgg gctctgccgg cggccgcggg acccgtccac
      121 gcccaacccg cctcaaccgg cggtcggcgt cgtatacgtc tgtgcgaaca gatcgatcgt
      181 gtgcgccact tcctccagtt cctgccgcgt caaccccggc gacaccggca gcgacagcac
      241 ttccttcgcc atctgttccg ccacggggat atggccctcc gtgtaaccca gatactcgaa
      301 acacggctgc aggtgcagcg gcaccggata atagacccgc gtgtcgacca tccggtcctt
      361 caaaaacgag cgcaactggt cgcgcatcgt gctgcggatc gtatagtagt tgaacacatg
      421 gctcccttcg ggccccggat cgggcagcgc gaacacctcg ctgctgaggt ggttctggta
      481 gaacgccgcg ttggcctcgc gatcggcgtt atcctcgtcc agcgtgccga gtttgacgtc
      541 caggatggcc gcctgaatcg cgtcgagccg gctattgtag ccgatctcca cgtagcgatt
      601 ttccgcctcc gcgccgtgat cgcgatacag ccgcagccgc tgggccagct ccgggttgtt
      661 cgtcacgatc gcgcccccgt cgccgatgca gcccaggttg cgctccgggc tgaaactgaa
      721 cgcccccgcg gttccccatg tcccggccgg ccggccgttg atctccgccc cgcaggcgtg
      781 cgaacaatcc tcgatcaggt gcaccccctg ttccgccgtc atctgcgcca gcgtctccac
      841 atccgccatc cggccgaaca tgtgggccgc gatcaccgcc cgggtgttct cgttcatcaa
      901 ctggcgcacg tgctccacgt cgatcgtaaa atgctcgttg atatcggcaa atacgatatg
      961 cgcccccatc cgcgggatca catcggccat cgccacatac ccgaacgcgg ggcagatcac
     1021 ctggtcgccc ggctgaatcc ccagcgcgtg cagcgtcagc agcagggcat cggtcccgga
     1081 gttgaccccc acacagtgac tcgccttgag ccgctgggcg aagttctgtt caaagcgcgc
     1141 caccgcactc cccaggatat actcgctgct gtcgatcacc tgctcgatcg cgtggagcaa
     1201 ctcctttttc agcggctcgt gctggcgact caaatccagg aatgatacgg ccatgatccc
     1261 tgtatcccat ctatcagagg agttagtcgg aattcctcgg ggaaccccgg cgaattgacc
     1321 agtatacagc atagggggat caattcgaat catcaaatgg agatatttac tagttaaata
     1381 agttaataag cgcagtcctg agtgggcgat gcgcccctcg tcccttaaac tcccccccgg
     1441 cctctccaaa tctctcggta aaccgccagc gtttcccgcg cggtgcgctc ccaactgaac
     1501 gcccccgccc gctccgcgga acgccggcgc caggccgcca gctcttcctc atcggtcagc
     1561 agccggagca gaatcccggc cagcgccgcc caatcggtgg ccgggaaaaa ctgggccgtc
     1621 ccccccgcga cctcgcggtg gatcggcagg tcgctcgcga cgatcgcgca cccggcgcgc
     1681 atcgcctcca ccagcgtcag gttgaacccc tcggtatgcg agcagctcac cagcaccgcc
     1741 gcccgccgca aaatgcgccg gacgaccgcg gatggcagcg cccccggcgc gcggaaaccg
     1801 gccttttccg cctgccggcg cagcgattcg ctcgaatcgt cgggcagcgg ccccgtgccg
     1861 ccgatccacc accactcgaa tgggggccgc cccgccggcc agcgccgcgc cagctcgatg
     1921 catgcccgcc gcgccgtcgc cgggttcttg tgcgggtcac gcgtcccgac ggttacgatc
     1981 agcggccccg ccgccggctc ctcaacgccc atctcctcgc ccccgccgtg ctcccacagc
     2041 atcggcgtgg cgtcggccgc ctcgcgcacc acccggatcc gcgcccgctc cgccaggccc
     2101 cgctcgacga tctgctcggc cacggtcgcc gaaacgctga tgatccggtc gctgaaccgg
     2161 caggcgcgcc ggatcgccca ccgcatccag gcccgaaact tcagcggaaa aaactgcgga
     2221 tgcgtgaaga cgaacaggtc gtgcaccgtg acaacccggc cgaatggctg ccggccgccc
     2281 gggacgatga acaccggccc gtgatagacg ctgccggccg gcagccgtcg gctcagtccc
     2341 cagcgcagcc agatgtcggt ctgcggatgg gcttcgtgcc cgaacatcgc cgccacgaca
     2401 tccacccgcg ccagcgccgg gtcgcgccgc gcaatttcaa ccgcgccggg cagaaacatc
     2461 gcccggatcg gcggcgcttc ctccagcgcc gccagccccg ccagcaggtt gtaggtatac
     2521 cgcccgacgc cggtcatccc ccggcggata ttgcgcgcat cgtaaaagat cgtcggagcc
     2581 ggcggcgcgt tcacgtcagg ccagctcgcg cagcgccgcg atcaattcgt ccagttgccc
     2641 cgcggtgttg tagaaatgcg gcgcgatgcg cagccggccg ccccgcggcg agaccgagat
     2701 ccgccgctcc tccagccggt tgaccagcgc ctggacatcc acgcccgccc gcgcggcgga
     2761 cgtgatcccc gagcgttccc cctcgccatc gggcgaaaca atcgtccacc ccgccgagcg
     2821 caaaccctcg cgcagccgcc ccgtcagcgc atccagccgc cgccagactt cgtcgatccc
     2881 gatctcattg agcagggcca gcgactggcc gagcgccgcc gcccccgcca tgttgtagga
     2941 accctcctcg aagcgccggg cgttgggcag cagcggctga tcgtagtcct cgtattgcga
     3001 gtagcggacg cgacccgtcc agccggtcat gcacacgttc atctcctcaa tccggtcggg
     3061 atggacccac aggatgccca acccttccgg tccgagcatc catttgtgcc cgtcggcgac
     3121 caggtaatcg cagcccagat cgaccggccg cgtcggcagc gcgcccaccg cctgaatcgc
     3181 gtcgacacag agccggaccc cgcggcggcg gcagatctcg gcgatccgct cgaccggcag
     3241 ccggcagccg ctcgcgtagt gcaccagaga cagcgcgacc agccgcgtcc gctcgtccaa
     3301 cagggccgcg aaatcctcgg cgcggtagcg ctgatccgga cccggctcga ccgcgcgcag
     3361 cgccaccccc tgctgcgcca ggttgcgcca gggatgaacg ttcgccggaa agtcggcggc
     3421 catgccgacg atgttgtcgc cggggcgcca cggcagcgaa ttggcgatgc acagcagacc
     3481 gtgcgtcgtg ttgtgaacaa acgccacgtc gtcgacctga acgtcaagca gccgggccgc
     3541 ctcggcgcgc gcggccttca gtcgcgcggc ccaccggccc cacagcgcgc cgcccccctc
     3601 gaccgcctcg tcggcgaact cccgcagcgc gcgccccgcc cgcgccggaa tcggcgccac
     3661 cgccgcatga ttcagataca cgcggtccct gaggatcggg aattcgtcat gcatcgggcc
     3721 gtcctggtcc atcatctcca cccttccaac aaaaaatcga ccctcgtcct cgcttcaaaa
     3781 tcgtcctcga ctgtcggctc tcgtccctcg gcctcggcct cgaaaatcgc ccccaaactc
     3841 cactccccca catccgctca ccatcgccgc tcggccttcc gtcggccatc gtcgccgatc
     3901 ttaattgttt caagcaggga aacggtttgc aaccgcggtc gggcccttcg ctctcgccgc
     3961 tttcgccgcc aaaccttctc tctcgttgcg cgctctctca tcctcgtctt cgtcttcgca
     4021 gggccgtcca cgccccctgc gcttcttgac attttttccc cgcgacgtca caagataggc
     4081 tccgctcggc gggtttttcg gccccggctg ggaatctcac ggcatcccgc cgccgggtcg
     4141 cgccgacccc attttcccca ggcctgcatc ccctcatgcc tcgttcccct gcccgcaccg
     4201 gctccgcaat ccgccgcctc cggcccatcc tgctggggct catcgtcctt tgtgcgttcg
     4261 ttctgcgcgt cacgttcaac acctaccagc ttccgcctta ctgggattac cccaacgaga
     4321 tcgacaagat ggtcgatgtg ctggcgctgg aggaactggg cttctaccac ggctacatgc
     4381 agccgtcgtt cctgatgaac acgatgtggc tgtggtatca gccggcgcag gtcgtcttcc
     4441 cgtgggtcaa cgcaacgttt cccggcttcc gtccgctgga ggggggcatg gaaggctggc
     4501 tgctctggct cgggcgggtt tatatggcgc tgttcggcgc ggcgacggtc tggctcgtgt
     4561 atttcgtcgg ccggcgcatc ggctcggcgc gcctgggcct taccgccgcg ctgctgctcg
     4621 ccgtcacgcc gatccatgtc ctcggctcgc gccacatgaa ggaggacatg ccgctggcgt
     4681 tcttcgtcac gctcgcgctg ctgtttctgc tcgatgtcgt gcggcggggc gcccgccgcg
     4741 actatctccg cgccggcctc gccgccggcc tcgccttcag caccaagtgg atcggcatct
     4801 ccatcctggc gccgttcctc ctggcgggcc tgctggcgat tccccgcacg cggcgcaact
     4861 ggcccgcccc cggccgccgc tggctgatcc cgctcatcct ggcctccttc gtcgccgggt
     4921 tcctcctcgc ctcgcccgac tacatcctgg ccgggcggat gaacatcatc tgggaaggcc
     4981 cgcaaaaggg tatgacgaag gcctacgatg cccacgccga cgggatcgcc gtcgacgcct
     5041 atcgcgaagc ctatgtcgat tatttccgca aggggatctg gccgggcctg accccggcgc
     5101 tgatcctcct cgccggcatc ggtttcggtt ccctctggcg gcggcgccgg tccgcggcgc
     5161 tcctgcttgg cttctgggtg gttttcttct acatcctcat tgaatcggcc cgcgcgcgcc
     5221 cctacccgca cttcgaacgc tacctgcaac cgatcacgcc gacgctgatt gttttcgcgg
     5281 cttgcggcgt cgaggccgcg cggcgcggcg ccctgcgcct cctgcggcag cgccgcggct
     5341 ttccgccgtt acggcgggcc gtcgccccgg cgctgctctg cctcgcgctc gcctggcccc
     5401 tcgccgacac catccgttat ctagaacaca tcccccgcag caccgtcctg caggcccgcg
     5461 agtggatcat gcagcacgtc ccccccggcg cgatcatcta cgacgacagc tacggccccg
     5521 gcttccccga aggacgctac gagcggcgca aataccggtc gaaggaccag ctcatcgtcg
     5581 gcgcgttccc gaccgaaccg tgttacgtca tgtatacgtc catgggactg gggcggttcg
     5641 tggaacaccc cgaggcgaat ccgggtgtcg cgcgctgggt cttctacgtc ctcagcaagg
     5701 gacggctgac caaagaatgg accccggaat tccgttcgtt ttacgccgaa agcccgctga
     5761 tcaagctcta ccggttcgag ccttcacccg acccgctggt cgtggagtat tgaacccacc
     5821 aaaaaaggaa atccgtcgga tcggtcggat ccgacggatt tcaactgaat gcctcgcgtc
     5881 tggaacgctt gccggtcagg gaaggatcgt gtgttcccgg ccgttgatct ccagcgtcac
     5941 cgaccccgga ttaatcgccg tgaccttagc gttgccaatg cgcgagccga cttccacgat
     6001 ctggccgttg atcagcgcca tcggccgcgt cttggcccag aagatccctt cgacccgcag
     6061 gccgtcggtc ggcgaggcgg gcgcctcgtg cttgggtttg cccgccggag cgggccgcgc
     6121 cgcccgcggc cgttccccct cggcggcatc gagcgacacc agccggccat tgatttccgg
     6181 aaccggcgcg gcttcataca acacttccgg cacgctggga tcgagcgcat aggccgacgc
     6241 cagctcgaat tcgaccgggg caaccggttc ggcggcggcg gcctccagct cggcgctcga
     6301 gacgaccgga acgatgccgg cctccggaac ggcgccgatc tcgaaatccg atcccatcgc
     6361 cgcggcggtt tgcgccggcc tccccgcccc agcccccgac gccgcgcccg gcgcggcgat
     6421 cgccgtctgc acctgtgctt ccaacgccgc gatccgcgac tccattgtct gcaccacgac
     6481 ctcttccagc ctgctggcga gcgaagccag cgcggcatcg gccgcccgcg gttc
//
LOCUS       DAOPCO010000227         6489 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10526_length_6489_cov_5.006994, whole genome shotgun sequence.
ACCESSION   DAOPCO010000227 DAOPCO010000000
VERSION     DAOPCO010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10526_length_6489_cov_5.006994"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..640
                     /locus_tag="PLS90_13415"
     CDS             335..640
                     /locus_tag="PLS90_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03444.1"
                     /translation="MTDEFFEVPCPECKSVLIIRRRDGKLMETRKPLLEETTGDRFED
                     AYERVRRAGNEIERKVAEAREREKGKMDRLNALFNDSLEKAKKEGPVQKPKREVDLD"
     gene            686..1558
                     /locus_tag="PLS90_13420"
     CDS             686..1558
                     /locus_tag="PLS90_13420"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPK03445.1"
                     /translation="MQLDCVLEAQSTLAEGPVWDPRAAVLWWVNIPLGELHRFDPQTG
                     EDVVFRLGEPLGAAVPRRHGGLVLALGGGFAFFDPARATAEPIAHPEADRPGNRFNDG
                     KCDPAGRFWAGTMPAGDPGPTGSLYCLDPDLHVERRETGVACSNGLAWTRDARTMFYI
                     DTPTRVVSAYDYDLATGAISNRRPVVEIPEGQGWPDGMTIDDNDNLWIALWGGSAVVC
                     HDPRTGKLLERIDVPVTHVTSCAFGGPDWRDLYITTARARWDRYELDPQPLAGNLFRV
                     RLPLPGRPTDLFAG"
     gene            1806..2867
                     /locus_tag="PLS90_13425"
     CDS             1806..2867
                     /locus_tag="PLS90_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK03446.1"
                     /translation="MKDSRRRTVSMRDIAERAGVSVATVSHVLRKTRKVNQETRQRVL
                     GVSRELGYEASDADREGVGFRPTHVLGLVVSDIQDPFFTESVKGFEDQAWLHHMEPVI
                     MNTNYDPLRAVSSIRRLIRIGVSGVAVLATEPHGAIIDHLTANDICAVYIDAGRVGPY
                     VASLNLDYAHGIRQAVRHLRELGHRRLGYIGSLLDQEAIRVRRQAFLEELGDAEGEVV
                     EAAGTVEGGYYIAGKLFSRSSPTAIVCSSDLLAIGALHYAADQGIQVPDQLSIVGFDN
                     IDFARFTMPELTTVGIPKYECGRLAFQVLNEMIQDPAHIGREVTVQPQLVLRQSTAPC
                     PEDPLAQPAGTGAETDPMI"
     gene            3094..4404
                     /locus_tag="PLS90_13430"
     CDS             3094..4404
                     /locus_tag="PLS90_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03447.1"
                     /translation="MKTRPLFLLAVLLFPLGSPLSAAVVQISGGGTALDGPWAAATPG
                     TIFEITDSLTYSPAVKLIAEAATHNNVTLRAAPSQTPTINFTIGAFDPFLVRAEVAGL
                     QIGSNSGGRIRMECGGGLLGIIQLNDARQVTLENLELVGVSGSSDCAVRIDAATPADS
                     SPRVFVINNCIFRNWNKLDDGVLYLREVPGSASVNDSYPASLDITITDCLFDNVIRAW
                     LVRQAGDHGAVTDRSKLHIENCRVINPTASGSLCIMFGDFIDVTIHNLLATRGSAGYS
                     FTAGDLFYGTGRGLNLTITRSAFNRGRWHVGQGATVVIDHCDMISETGGIYIDPSATG
                     SVSATNSNFISTAGSSLFSQAAGSPTLISDYNNCPQGYSGWTAGPHDVSISDPGYADV
                     PNDWRVSASALVGDASDGGDIGSAIFLDPFDNVVPVELSEFVVN"
     gene            4536..5171
                     /locus_tag="PLS90_13435"
     CDS             4536..5171
                     /locus_tag="PLS90_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03448.1"
                     /translation="MSHHAVPGNRGFTLIELLIVVAIIAILAAIAVPNFLEAQMRARI
                     SAVKADHRSVLTAWGIYRVDYNAFPIVDGRTWPTRLTTPVAYMTSVPEDWFFNFGPYS
                     HSPLFQRYYPRPNYFNWLAREHWGVTGKGAGVHGKVFTPPYDFYWENTWRFYSPGSSM
                     AISAYGPSPGDSYFFFGNTNTSRYPGSTGTVVGRYDPTNGTISMGHILSFD"
     gene            5226..>6489
                     /locus_tag="PLS90_13440"
     CDS             5226..>6489
                     /locus_tag="PLS90_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03449.1"
                     /translation="MNRFFCIALLLAVAISSAAAQTVTVSGGGTAFQTVWNNATPGIT
                     IEITDSLTYLTDAVLVPATPATHNNVTVRAAAGQTPTIRILHDGALIGLRIHSGVENL
                     KIGSNDGGRITFRFEELVYFGIKLVDVRSVTLENLDLIGTTATADTAVYITQSLPAQT
                     QVVNTIVVDNCLFRAWVLSDGAVTVHETPLGGLYNAANDITFRNCVWEENVLDLRLRA
                     GNDHGSFATRSTWTLENCRILNTDDTPGSWGGFHGEWWDVILDRTYIQYAASSGGGVG
                     DLNYNTHLGVGFQARDSVFINAGLSLALSSQVSIDHCDFIMQSTPITLAEYVDSEENI
                     RSFEGVANIMNTNFINQGSVGFSMGDTTTEGNPTLLNDYNNCPQGYQGKWVAGPHDQA
                     VNPNYVAFPDNLKVQAPELIKAGSDGRDI"
BASE COUNT         1167 a         2299 c         1850 g         1173 t
ORIGIN      
        1 accgcgcagc ggtcgtcgac gccgctttag tatcgccatc gccctctccc cttgcccgac
       61 gtcattttgt ggagtgcggc ggcagagacc gcgcagcggt cgtcgacgcc gctttagtat
      121 cgccatcgcc ctctcccctt gcccgacgtc gttttgtgga gtgcggcggc agagaccgcg
      181 cagcggtcgt cgacgccgct ttggatcacc ttcctgatcc cgatcccggt cccgatcccg
      241 gcggcgcgaa aaaggcttgg gtccccgcgc cgccgatcta taatgaaacg gcgatctccc
      301 cgccgccggc gcggccagga aggtgatggg cgccatgacc gacgagttct ttgaagtccc
      361 ctgtccggag tgcaaatccg tcctgatcat ccgccgtcgc gacggcaaac tgatggagac
      421 gcgcaagccg ctgctggagg aaaccaccgg cgaccgcttc gaggatgcct atgaacgcgt
      481 ccgccgcgcc ggcaacgaga tcgaacgcaa ggtggccgaa gcccgggaac gcgaaaaggg
      541 aaaaatggac cgcttgaacg ccctgttcaa cgacagcctg gaaaaggcca aaaaagaagg
      601 cccggtccag aaacccaaac gcgaagtcga cctggactag atccgcgtcc ccccaattcc
      661 acttgcccgg agcggagcgc gcacgatgca actggactgc gtcctggagg cgcaatcaac
      721 gttggccgag ggcccggtct gggacccccg cgcagccgtc ctctggtggg tcaacatccc
      781 cctgggcgag ctgcaccgct tcgatccaca aaccggcgag gacgtcgtct tccggcttgg
      841 cgaaccgctc ggcgccgccg tcccgcgccg ccatggcggc cttgtcctgg cgctcggcgg
      901 cggcttcgcc ttcttcgacc ccgcccgcgc gacggccgaa cccatcgccc atcccgaggc
      961 cgatcggccc ggcaatcggt tcaacgacgg caagtgcgat ccggccggcc gcttctgggc
     1021 cggcacgatg cccgccggcg atcccggtcc caccggcagc ctctactgcc tcgatcccga
     1081 cctccatgtg gaacgccgcg agaccggcgt ggcctgctcc aacggcctgg cctggacgcg
     1141 cgacgcccgc acgatgttct acattgatac cccgacccgc gtcgtctcgg cctacgacta
     1201 cgatctggcg accggggcga tctccaatcg ccgccccgtc gtcgagatcc ccgaaggcca
     1261 ggggtggccc gacggcatga cgatcgacga taacgacaac ctctggatcg ccctctgggg
     1321 cggctcggcc gtcgtctgcc atgacccgcg caccggcaaa ctcctcgaac ggatcgacgt
     1381 cccggtcacc cacgtcactt cctgcgcctt cggcggcccc gactggcgtg acctctacat
     1441 caccacggcc cgcgcgcgct gggaccgcta cgaactcgac ccccagccgc tggcgggcaa
     1501 cctcttccgc gtccgcctcc ccctccccgg ccgccccacc gacctcttcg ccggctgatc
     1561 ctcccccggc gagaatcgtc ctcgttctcg tccctcgtcc tcggttctcg cccctccaag
     1621 cccctctctc gctgtccgcc ctcgtctctc gctcacgcac tgccgcgccg ggaatccccc
     1681 gcccgccgaa aactgcattt ttccgcttga cgcccggccg ggcctcagct aaccttgcgc
     1741 aacatcacgt aacgtttcca ttcagccccc ctcccccgcg gaggtaacgg catggacggc
     1801 accgcatgaa agattcgcgc cggcgcacgg tcagcatgag ggacatcgcc gaacgggccg
     1861 gggtttccgt cgccaccgtc tcgcacgtcc tgcgcaaaac ccgcaaggtg aaccaggaga
     1921 cccgccaacg cgtcctgggc gtctcgcgcg agctcggcta tgaagccagc gatgccgatc
     1981 gcgagggagt cggcttccgc cccacccatg tcctcggcct cgtggtttcc gatattcagg
     2041 acccgttctt caccgaatcg gtcaagggct tcgaggacca ggcctggctc caccacatgg
     2101 agccggtcat catgaacacc aattacgatc cgttgcgcgc cgtcagcagc atccggcgcc
     2161 tgatccggat cggggtctcc ggcgtcgccg tcctcgcgac cgaaccgcac ggcgcgatca
     2221 tcgaccacct gaccgccaac gacatctgcg ccgtttacat cgatgccggc cgcgtcggcc
     2281 cgtatgtcgc cagcttgaac ctggactatg cccacggcat ccgccaggcc gtgcgccacc
     2341 tgcgcgaatt gggccaccgc cggctgggct acatcggcag cctgctggat caggaggcca
     2401 ttcgggtgcg ccgccaggcc ttcctggagg aactgggcga tgccgagggc gaagtcgtcg
     2461 aggccgccgg cacggtcgag gggggctact acatcgccgg caagctcttc tcgcgctcct
     2521 cgccgacggc gatcgtctgc tccagcgatc tgctcgcgat cggcgcgctc cattacgccg
     2581 ccgatcaggg catccaggtc cccgaccagc tttcgatcgt cggcttcgac aacatcgatt
     2641 tcgcccgatt caccatgccc gaactgacca cggtcggcat ccccaaatac gagtgcgggc
     2701 ggctggcctt ccaggttctg aacgagatga ttcaggaccc ggcgcatatc ggccgggaag
     2761 tgaccgtcca gccgcagctg gtcctgcgcc agtccaccgc gccctgtccg gaggatccgc
     2821 tggcccaacc cgcgggcacg ggcgcggaaa ccgatccaat gatttagcgc gcattgaatt
     2881 ccgaaccgga aaggaggcgg cgaaccaaac ctgacatcaa acacacttaa caagatcctg
     2941 acgtttgatt gcccgacccg gccccgctga tccacgcccc gctgacacca accgggaaac
     3001 cccttcaaca ggctgtccgg aaggaaacgg cgcccccagc gaccgtcccc ctccggcacg
     3061 tggaagtagt gcccatacca ggaggcattg gccatgaaaa cccgtccgct gtttctgctg
     3121 gcagtcctgt tgttcccgct tggctccccc ttgagcgccg cggtggtcca gatttccggc
     3181 ggcggcacgg cgctcgacgg cccctgggcc gccgccacgc ccggaacaat ctttgaaatc
     3241 accgatagcc tgacttattc gcccgccgtc aagctgatcg ccgaggccgc cacgcataac
     3301 aacgtcacgc tccgcgcggc cccaagccag acgccgacca tcaacttcac catcggggcg
     3361 ttcgatccgt tcctcgtccg ggccgaggtc gcgggcctcc agatcggctc caacagcggc
     3421 ggacgcatcc gcatggaatg cggcggcggg ctgctgggca tcatccagct gaacgacgcc
     3481 aggcaggtca cgctggagaa cctggaactc gtcggcgtca gcggctcgtc ggattgcgcc
     3541 gttcgcatcg acgcggccac tcccgccgac agcagtccgc gtgtctttgt cattaacaat
     3601 tgcatcttcc gcaactggaa caagctcgac gacggcgtcc tctatctgcg cgaagtgccc
     3661 ggcagcgcca gcgtgaacga ttcctacccc gcctcgctgg acattacgat caccgattgt
     3721 ttgttcgaca atgtgatccg tgcatggctc gtgcgccagg ccggcgatca cggcgcggtg
     3781 accgatcgca gcaaactcca catcgagaat tgccgcgtga tcaatccgac cgccagcggc
     3841 tcgctctgca tcatgttcgg tgatttcatc gacgtgacga ttcataacct gctcgccacg
     3901 cgcggctcgg cgggctacag ctttacggcc ggggacctgt tttacggaac gggtcgcggc
     3961 ttgaatctca ctatcacccg ttcggccttc aaccgcgggc gctggcacgt cggccagggc
     4021 gccaccgtcg tgatcgatca ttgcgatatg atttccgaaa cgggcgggat ctatatcgat
     4081 ccctcagcca ccggcagcgt ctccgcgaca aactccaact tcatctccac ggccggttcc
     4141 tcactgttct cgcaagccgc gggcagcccg acgctcatca gcgattacaa taactgcccc
     4201 cagggctaca gcgggtggac cgccggaccc catgatgtgt caatttcgga tccgggttat
     4261 gccgacgttc ccaacgactg gcgcgtcagc gccagcgcgc tggtcggcga cgcctccgac
     4321 ggcggcgaca tcggttcggc gattttcctc gatccattcg acaacgtggt gccggtggaa
     4381 ttgtcggagt ttgtagtcaa ctgaatgccc cgtaacccct gtcggacgtc gggccgggcc
     4441 gggagcggtc gctcccaccg gtcgccccac cggcccggcc gcccctcctc cgttcgagca
     4501 agccccgcgt tgacgcgggc gggagatcga tcaccatgtc gcaccacgcc gtgcctggaa
     4561 atcgaggttt cacgttgatt gaactgttga ttgtcgtggc gatcatcgcg atccttgcgg
     4621 ccatcgccgt ccccaatttc ctcgaagcgc agatgcgtgc ccgcatctcc gccgtgaagg
     4681 ccgaccaccg ctccgtgctc accgcgtggg gcatctaccg cgtggattac aatgcctttc
     4741 cgattgtcga cggccgcacc tggccgacgc ggctgacgac ccccgtcgcc tacatgactt
     4801 ccgtcccgga ggactggttc ttcaactttg ggccctattc gcactccccc ctgttccagc
     4861 gctactaccc ccggcccaac tatttcaact ggctcgcccg ggaacactgg ggcgtgacgg
     4921 gaaagggcgc gggcgtgcac ggcaaggtgt tcaccccgcc ctatgatttt tattgggaaa
     4981 acacgtggcg gttttactcg cccggctctt cgatggcgat ctcggcctat ggaccttccc
     5041 cgggtgactc ctatttcttc ttcggcaaca ccaatacctc ccgctatccc ggttcgaccg
     5101 ggacggtcgt cgggcgctat gaccccacca atggcaccat ctcgatggga catatccttt
     5161 cgtttgatta ggacgcaatg gcatccgcca ggcaacatca ccccatgaag gaaaggaaca
     5221 gcatcatgaa ccgatttttt tgcatcgcgc ttctgctcgc ggtcgccatt tcatcggccg
     5281 cggcacagac cgtcaccgtt tcgggcgggg gaaccgcctt ccagacggtg tggaacaacg
     5341 ccacgcccgg catcacgatt gaaattaccg acagcctgac ctatctcacc gatgccgtgc
     5401 tggtgccggc cacacccgcc acgcataaca atgtcaccgt ccgcgccgcg gccgggcaga
     5461 ctcccacgat ccgcatcctg cacgacggcg cgctgatcgg tctgcgcatt catagcggcg
     5521 tggaaaacct caagatcggc tccaacgacg gcggtagaat taccttccga ttcgaggagc
     5581 tggtttactt cggcatcaag ctggtcgatg tcaggtcggt gacattggaa aatctggacc
     5641 tgatcggcac cacggcgacc gccgacacgg ccgtgtatat cacgcaaagc ctgccggccc
     5701 agacccaggt ggtcaatacg atcgtcgtcg acaactgcct cttccgcgcg tgggtcctca
     5761 gcgacggcgc ggtgacggta catgaaaccc cgctgggcgg cctctacaac gcggcgaacg
     5821 atatcacgtt cagaaattgc gtctgggaag agaatgtcct ggatctccgc ctgcgcgccg
     5881 gcaatgatca tggctccttc gccacccgca gcacctggac cctcgagaac tgccggatcc
     5941 tcaacaccga cgacaccccg ggtagctggg gcggcttcca tggcgaatgg tgggatgtca
     6001 tcctcgaccg gacttatatc cagtatgctg cttcgtccgg cggcggcgtc ggcgatctga
     6061 actacaacac ccatctgggc gtcggctttc aagccaggga ctccgtcttc atcaacgccg
     6121 gactttccct cgcgctgtcc agtcaggtca gcatcgatca ctgtgatttt atcatgcaaa
     6181 gcacgccgat cacgctggcc gaatacgttg actcggaaga gaatatccgg tcgtttgaag
     6241 gcgtcgccaa tatcatgaat accaatttca ttaatcaggg ttctgtcggc ttttccatgg
     6301 gagataccac aaccgaggga aatcccacgc tgctgaatga ttacaataac tgcccccagg
     6361 ggtaccaggg caaatgggtc gcgggccccc atgatcaggc ggtcaatccc aattatgtcg
     6421 cttttccgga taatttgaag gtccaggcgc ctgaactgat caaggcgggc agcgatggcc
     6481 gggatatcg
//
LOCUS       DAOPCO010000228         6458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10578_length_6458_cov_4.617992, whole genome shotgun sequence.
ACCESSION   DAOPCO010000228 DAOPCO010000000
VERSION     DAOPCO010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10578_length_6458_cov_4.617992"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLS90_13445"
     CDS             complement(<1..199)
                     /locus_tag="PLS90_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03450.1"
                     /translation="MKKYCLILGLLLLLAGKTSAEDNGLLALLQDYDNLFLADYSVDL
                     KVTQPTGIMPEDGMSTCQVSLT"
     gene            complement(251..730)
                     /locus_tag="PLS90_13450"
     CDS             complement(251..730)
                     /locus_tag="PLS90_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03451.1"
                     /translation="MIGSQIIMIGALILQALAPAPERVETLSDAAMAELIGGDGCKNC
                     HYAPDRFDECLHYTQSDICQTSVCIANYLIESTCDLGAGTCTAYSNAYAAGYVQYARM
                     DYYCSTSNPTQWLIYTTHYYGPNCTAAHHFMRCNKPTNNCNGTLVGSATAYPGISCQ"
     gene            complement(737..2242)
                     /locus_tag="PLS90_13455"
     CDS             complement(737..2242)
                     /locus_tag="PLS90_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPK03452.1"
                     /translation="MRRLPIPNLQVLLFLLICRSLAAQAPNPPEGSAVDPHAGHADGH
                     SDAELDKSCGLRSLILAGHKLGIPLKFEDLLPEVEFQEAGLSMLQLAQLARSHGLTAE
                     GQRIDWATLRSLGQPAILFVRGSHFILADPTVVHHRRPDELIRYYEPPDVAGWLTRAQ
                     LEEQWAGHALVLGRDPAATDSIYRLGPIYHDFGNATENDDIRFQYTLTNQSDLPITLS
                     EPHLSCGCTSATLSTRTVQPGQTAYLDLFQDLNGVSGQKNFDIGVQINPPDGPHVGFT
                     LTGYVEQGVRLSHDVVRLKHLKPGVDVTETVYFEDMTGAGVEVLEAWVEGNCGDATLT
                     PVVEPVEPEGDITSRYAIHLLVSPPQAMPAGCTARLMLHTTSEDNAILEVPVELAMLP
                     TLSADVDTLAFGVLTPGDERSEVLRIASEDQGALESLKLEVNAEQPGYPVPEITGTEA
                     GEQSFGATLRLRVPADLAVAEKMFIKGHISARNGEGESLEIPYLYLIEPKG"
     gene            complement(2258..2599)
                     /locus_tag="PLS90_13460"
     CDS             complement(2258..2599)
                     /locus_tag="PLS90_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03453.1"
                     /translation="MGIATIEPIAIEDRKPSFIDDPSSGYSYEHVSIVRVAAPGEAWD
                     PIVRKLTEKTDKLAFFLPMLSSCSLEQQFNLLVNVRDALHDEFKDLPPTVLEVLQSAL
                     DYGYDYVWLQF"
     gene            2834..3394
                     /locus_tag="PLS90_13465"
     CDS             2834..3394
                     /locus_tag="PLS90_13465"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK03454.1"
                     /translation="MDEPAPDPASAAEKEWVIRAMAGDKDAFGRLFDLYNKRLAHYLR
                     RLIAQPEDIHDVLQDVWLAAYLKLPRLRKPEAFRSWIFTLARNRALDLRKVNRREWNL
                     TEEDLEELEETPQRGDLPHAELEILRLAMEKLKPKHREVIFLFFFEAMPIKEIAGVVG
                     CDDGTVKSRIHYAKQHLKKAIMRLSQ"
     gene            3391..3813
                     /locus_tag="PLS90_13470"
     CDS             3391..3813
                     /locus_tag="PLS90_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03455.1"
                     /translation="MNVTLTDLDRMYDGEAGGAGQHELLKAQVFGAIRRHETKTRILK
                     GASICSWIMFFGLAGMLSLNLVKHNQRSSDSMNAPFYQGGSYWYADSGSAPMEPLGVI
                     DLLAGWPGVVAAILLTAAICTTVIFLIRRRPTPRRLPI"
     gene            4109..4371
                     /gene="ffs"
                     /locus_tag="PLS90_13475"
     ncRNA           4109..4371
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS90_13475"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4467..4736)
                     /locus_tag="PLS90_13480"
     CDS             complement(4467..4736)
                     /locus_tag="PLS90_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03456.1"
                     /translation="MSEIHVAHHEGNRYEVTVKGGKTTTHHVDLPRDYYLKLSGGKIS
                     EEDLIRRSFEFLLKHEPNTAIFPRFELATINRYFPDFEATMTREI"
     gene            complement(4832..5977)
                     /locus_tag="PLS90_13485"
     CDS             complement(4832..5977)
                     /locus_tag="PLS90_13485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK03457.1"
                     /translation="MLEAWQFPEPIFVTRPTLPSLADYTRQLEAIWESHKLTNNGPLH
                     RRLEEALTRRLDVEHLSLTCNGTIALLIALQALRINGGEIIVTPFTYPPTVHVLYWLQ
                     VKPVFCDIDPLTWTIDPARIEALITPETRAIMGVHVYGRPCAVDEIQAIADRHGLHVI
                     YDAAQAFGVEVDGRSVLRWGDMSTLSFHATKLYSTIEGGAIVTNTPERKQRVDFLKNF
                     GIADEETVIGPGINGKLNEMQAAYGLLRLDDVDREIERRRELTAIYRRELDRVPGLTL
                     LPDQPGVRHNFAYLPVLVDPEACGLSRDRLYDLLKEFNVHARKYFHPLCSSYPCYRGL
                     PTARPDHLPVATRIASHVLCLPLYGTLAPEEAGRICAIIRGLIELTT"
BASE COUNT         1164 a         1938 c         2122 g         1234 t
ORIGIN      
        1 cggtcaggct cacctggcag gtgctcatgc catcttccgg catgattccg gtcggctggg
       61 tcactttcag gtcaaccgag taatccgcca gaaagaggtt gtcgtaatcc tggagcagtg
      121 ccaacaggcc gttatcttcg gcagaggtct ttccggccag aagcagaagg agaccgagaa
      181 tcaggcaata ctttttcatg ctgatcagcc tttcggatct ttcagaaacg tggttcatgg
      241 aatgggtgac ttactggcag gaaatgccag gataagccgt cgcgctgccc acgagggtgc
      301 cattgcaatt gtttgtgggc ttgttacagc gcatgaaatg atgggcggcc gtgcaattcg
      361 gaccgtagta gtgagtcgtg tagatcagcc actgagtcgg attggatgtc gaacagtagt
      421 aatccatccg tgcatactga acgtatcccg cggcataggc attcgagtag gcggtgcatg
      481 tgccggcgcc caggtcgcac gtgctttcga tcaggtagtt ggcgatgcag acgctggtct
      541 ggcagatatc gctttgagtg tagtgcaggc actcgtcaaa ccggtcgggc gcgtaatggc
      601 agttcttgca gccgtcgccg ccgatcagtt cggccatggc cgcgtccgag agcgtttcga
      661 cgcgctcggg cgcgggcgcc agggcctgca gaatgagcgc gccgatcata atgatctgcg
      721 atccgatcat ggttccctat cctttcggtt caatgagata aaggtaggga atctccaagg
      781 attccccctc cccgttgcgt gctgatatgt gccccttgat gaacatcttc tccgccaccg
      841 ccagatcggc ggggacccgc aaccgcagcg tcgcgccgaa gctctgctcg ccagcctcgg
      901 tgccggtgat ctccggcacg gggtagccgg gctgttcggc gttgacttcg agcttgagcg
      961 actcgagggc gccctgatct tcgctggcga tccgcagcac ttcagatcgc tcatcgccgg
     1021 gggtcaggac cccgaacgcg agggtgtcga catcggcgga gagcgtcggc agcatcgcca
     1081 gctcgaccgg cacctccaga atcgcgttat cctccgacgt cgtatgcagc atcagccggg
     1141 ccgtgcagcc cgccggcatc gcctggggcg ggctgaccag caaatggatc gcgtagcgcg
     1201 aggtgatgtc accttccggc tcaaccggtt cgaccaccgg cgtcagcgtg gcgtcgccgc
     1261 agttgccctc gacccaggcc tccagcactt cgactccggc gccggtcatg tcttcaaaat
     1321 agaccgtttc ggtgacgtcg acccccggtt tgaggtgctt cagccgcacg acgtcgtgac
     1381 tcagccgcac gccctgctcg acatatccag tcagcgtgaa cccgacgtgc ggaccgtccg
     1441 gcgggttgat ctgcacgccg atgtcgaagt tcttctgacc ggaaacgccg ttgagatcct
     1501 ggaacaggtc gagataggcc gtctggcccg gctgcacggt tcgggtgctg agcgtcgcgc
     1561 tggtgcagcc gcacgacaga tgcggctcgg acagcgtgat cggcagatcg gactgattcg
     1621 tcaacgtgta ttggaaccgg atatcgtcgt tttcagtggc gttgccgaaa tcatgataga
     1681 tcggtcccag ccggtagatc gaatccgtcg ccgccggatc gcgccccaga acgagcgcgt
     1741 ggcccgccca ttgttcctcc agttgcgccc gcgtcagcca gcccgcaacg tccgggggct
     1801 cgtagtagcg tatcagctca tccggccggc ggtgatgaac cactgtcgga tctgcaagga
     1861 tgaaatgaga gccgcggaca aagagaatcg cgggttgccc gagggagcgc agcgtcgccc
     1921 agtcgatgcg ttggccttcg gcggtgagcc catggctccg cgccagctgg gccagttgca
     1981 gcatgctgag cccggcttcc tgaaattcca cttcgggcag caggtcctcg aacttgagcg
     2041 gaataccgag cttgtgaccg gccaggatca gactgcgcaa gccgcacgat ttatccagct
     2101 cggcgtccga gtgcccgtcg gcatgaccgg cgtgcgggtc cactgccgat ccctcgggcg
     2161 gattgggggc ctgcgccgcg agggatcggc agatcagcag aaacagcaag acttgtaggt
     2221 tcggaatggg cagtcgacgc actatcacgc tcttcgctca gaattgcagc cagacgtaat
     2281 catagccgta atcgagcgcc gattgcagca cctccagcac ggtgggcggc aaatccttga
     2341 actcgtcgtg cagcgcgtcg cgcacgttga ccagcaggtt gaactgctgc tcaaggctgc
     2401 acgaggaaag catcggcagg aagaaggcca gcttgtcggt cttctcggtc agcttgcgga
     2461 cgatcggatc ccacgcctcg ccgggcgcgg cgacccggac gatcgagaca tgctcgtagg
     2521 agtaacccga tgatggatcg tcgataaacg agggcttcct gtcctcgatc gcgatcggtt
     2581 cgattgttgc gattcccatg ttcccctcca gattggatgc ctcggcagcg agggtctgga
     2641 tccgtctact gagtaagacg atcaagggga gccggcggtt caaattattt ttcaagatct
     2701 tgggtgtggc ccgccaagcg ccgattatgg caaagtgacc aatgagggag cgaccgtgtg
     2761 cttttttgtt ggcaactcgc gctaattcac gttgcaattt caggaagaca tgattagcat
     2821 tgcctcagaa tccatggatg agcccgcccc cgatcccgcc tccgcggcgg aaaaggaatg
     2881 ggtcatccgc gcgatggctg gggacaagga tgcttttggc cgcctgttcg atctttataa
     2941 caagcgcctg gcacattacc tgcgccgcct gatcgcgcag ccggaggaca ttcacgatgt
     3001 cctgcaggat gtctggctgg cggcctatct gaagctgcct cgtctgcgca aaccggaggc
     3061 attccgatcc tggatcttca cgctggcgcg caaccgggcg cttgatctgc gcaaggtcaa
     3121 ccggcgcgag tggaatctga ccgaggagga tctggaggaa ctggaggaga cgccccagcg
     3181 gggcgacctg ccccacgccg agctggaaat cctgcggctg gcgatggaga aactcaagcc
     3241 gaagcatcgc gaagtgatct ttctgttctt ttttgaagcc atgccgataa aggaaatagc
     3301 cggtgtcgtc gggtgcgatg atggaacggt caagtcccgg atccattacg ccaaacagca
     3361 tttgaaaaag gcgatcatga ggctgtcaca atgaacgtta cgctgacgga cctggaccgg
     3421 atgtatgacg gcgaagccgg tggcgcgggg cagcatgagc tgctcaaggc gcaggtgttc
     3481 ggcgccatcc gacgccatga gacgaagacc cgcatcctca agggcgcgtc gatctgctcg
     3541 tggatcatgt ttttcgggct ggccggcatg ctgagcctga acctggtcaa gcacaatcag
     3601 agaagttccg actccatgaa cgctcccttt tatcaagggg gaagctactg gtatgctgac
     3661 agtggctccg cgccgatgga gccgctgggc gtaatcgacc tgctggccgg ttggccgggt
     3721 gtcgttgccg cgatcctgct gaccgccgcc atctgcacga cggtcatctt tctgatccgg
     3781 cgccgcccga cgccgcgtcg cctgccgatc tgaagccgcg gccggtccgg gcacgaggtt
     3841 caaggatcct aacggcggtt gccggcgccc gtgtcgccag ccggcgggat gatttgtata
     3901 tatacgcgat tccggcatcc cccgcgggtg cttccctgac tcgatttcga ccccgatggc
     3961 gataccgaaa ccctggctcg agggttggcg aattcttgac cagttcgggc cattgacccc
     4021 ggcgggccct gggcgtagac taaggtctgt tcgtgcctgc ccgcccgcgg gcgtgtgggc
     4081 gcgcggcgcg aggcatttca ccgtttggtc gcgctaggcg gggagccagc ggtgccctgt
     4141 acctgcaatc cgctccagca gggctgaatc cccgcccgag ggaaagggtg gtcggtggac
     4201 cgggtccacg gagtgttgat cccggagtcc cgcgcaagtg gccccttcga accgggtcag
     4261 gacctggcgg tagcagccct aaggagtcag gccattgtgc gtcgggagcg cttcggggga
     4321 gccaccgggt cccgtcaagc cggttgttct ttttcgacgg cgggtgcgcg accattaaca
     4381 ccaacggccg gtccggcata catgccggat cggccgttcg agttttacag ggcgtcagac
     4441 ctgatccgcg aaggtggcat cccgcttcat atctcgcgcg tcatcgtcgc ctcgaaatcg
     4501 gggaagtagc ggttgatcgt cgcgagctca aaacgcggaa agatcgcggt gttgggttcg
     4561 tgtttgagca gaaattcgaa tgagcggcgg atcagatcct cctcgctgat ctttccgccc
     4621 gacagcttga ggtaataatc ccggggcagg tccacgtggt gagtcgtcgt cttgccgccc
     4681 ttgacggtca cttcatagcg gttgccctca tgatgcgcga catggatctc ggacatgcgc
     4741 gtgtcctccc gctgatgtta attcacgatg atctctccca tttactcaac tttcgcggac
     4801 gggggcaagc gccgtcccca gcgggcgagg atcaggtggt cagttcaatc agtccacgga
     4861 tgatggcgca gatccggccg gcttcctccg gcgcaagcgt tccatacaag ggcaggcaaa
     4921 ggacgtgcga ggcgatgcgc gtggcgacgg gcaggtggtc gggccgcgcc gtgggcaggc
     4981 cgcggtagca cggatagctg ctgcaaagcg gatgaaaata cttgcgcgcg tggacgttga
     5041 actccttgag cagatcgtag agccggtcgc ggctgaggcc gcacgcctcg ggatcgacga
     5101 gcacgggcaa gtaggcgaag ttgtggcgca cgccgggctg atcggggagc agggtcagtc
     5161 ccggcacccg gtccagttcg cgccggtaga tggcggtcag ctcgcggcgc cgttcgattt
     5221 cccgatccac gtcgtccagg cgcagcaggc cgtaggccgc ctgcatttca ttcaatttgc
     5281 cgttgatgcc gggcccgatc accgtctctt cgtcggcgat cccgaaattc ttcagaaaat
     5341 cgacgcgctg cttgcgttcg ggagtgttgg tgacgatggc gcccccctcg atcgtcgagt
     5401 agagtttggt ggcgtggaaa ctcagcgtgg acatgtcgcc ccagcgcagg accgaccgtc
     5461 cgtcgacttc gacgccgaag gcctgcgcgg catcgtagat cacgtgcagc ccgtgccggt
     5521 cggcgatggc ctgaatctca tctacggcgc aggggcgccc atagacgtgc acgcccatga
     5581 tcgcgcgggt ctcgggcgtg atcagcgcct cgatgcgggc cggatcgatc gtccaggtca
     5641 gcggatcgat gtcgcagaag accggcttga cctgcagcca gtagaggaca tgcaccgtcg
     5701 gcggataggt gaacggggtg acgatgatct cgccgccgtt gatccgcagg gcctgcagcg
     5761 cgatcagcag cgcgatcgtg ccgttgcatg tcaggctcag gtgctcgacg tccagccggc
     5821 gcgtcagcgc ttcctccagc cggcggtgga gcgggccgtt gttcgtcagc ttgtggcttt
     5881 cccagatcgc ctcgagctgg cgcgtgtagt cggccagact gggcagcgtt ggccgcgtca
     5941 cgaaaatcgg ctcggggaac tgccatgcct ccagcatctc ggccaggcgc tcggcctggc
     6001 gggcgcgcag cggcagcggc gcccggtccg gcgcgggcgc gagggatgga tcggacgcgg
     6061 gcaacgagag ggtcgaaagc atcgcggaac acccgggagc ggggatgggg cgcaatcggg
     6121 cccgctcaac ttatcggccg cgccggccgg ccgggtgagc cggttgggcg gcggaacggc
     6181 cacgcccggc gggccctttg cggtggcgcg cggcggcgcg tttccggcaa gatggcgcag
     6241 ccatgagtcg acccgccgcc agaacgcgcc cgaaagccgc gaaatccgcc ggtcgcgcgc
     6301 gcccgtttcc aattcacgag atgatgcgcg cgctgcggcg tgaagtggag cggctccccc
     6361 ggccgtacgt aacggaggaa gcctgggccc gtcacgatcc attccgggtc ctgatctcga
     6421 ccatcctgag cctgcggacg aaggatgaga cgacggcc
//
LOCUS       DAOPCO010000229         6458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10579_length_6458_cov_4.448696, whole genome shotgun sequence.
ACCESSION   DAOPCO010000229 DAOPCO010000000
VERSION     DAOPCO010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10579_length_6458_cov_4.448696"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLS90_13490"
     rRNA            <1..338
                     /locus_tag="PLS90_13490"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            453..568
                     /gene="rrf"
                     /locus_tag="PLS90_13495"
     rRNA            453..568
                     /gene="rrf"
                     /locus_tag="PLS90_13495"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(723..1709)
                     /locus_tag="PLS90_13500"
     CDS             complement(723..1709)
                     /locus_tag="PLS90_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK03458.1"
                     /translation="MKYRRLGRTGLAVSPICLGTMGYGPPTEQADVDHMVRLALDHGI
                     NFIDTANCYDGPNRGEIVLGTSESMLGKALEGLRDDVVLLTKAAVPLRPGPQHRGLSS
                     THLQREIDNSLRRLRTDYVDIFMIHWPDPFADTEEVLRAIDRIVSSGKARYFGISNHQ
                     GWEVCEYLWKADRRGWPMAGVSEVPLSILDRRYENDLPFYEKNQIGVIPYQPLKAGIL
                     SGRYRRVGMAGNEDRAIAGWEPEMNDAVLDQVEALMELAEGYGVTLAQFALAWDLAQP
                     AVSSVIVGARADRDLISALEADRIARELTADTLAAVDRIAPGPVRPAPRFER"
     gene            1930..3033
                     /locus_tag="PLS90_13505"
     CDS             1930..3033
                     /locus_tag="PLS90_13505"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HPK03459.1"
                     /translation="MSVLVLADDLTGLAEVACQAARYGLGGPMALTPAAPRGQPDTEN
                     NSRLFVNLGCRALPPAEAGERIRDALAGPLKSDGGDRVFLKIDSTARGPIPAMLAALH
                     AACPETTIPLCLVNPATGRSVREGRLHVKGDPVDRTEFARDPLHPVTDACLPRWLAHP
                     ELRVERESRSPSGGRDVAILDGETDADLAAATRRWADSPVAAGTAAFGLALLRSWSGS
                     PAPAPAPQWTVPPNTLVVVGTKSPSGRALIEDFGQTGRRAAPASEGVQTAAPRILHSP
                     AEDASPEAVLAKLVRRTEEAVSRRRPDLLIILGGETALAIIRALRLDWARPVAYRDGL
                     GWIEARHEGRPLPIWLMPGAYYRRGLWRELATR"
     gene            complement(3256..5223)
                     /locus_tag="PLS90_13510"
     CDS             complement(3256..5223)
                     /locus_tag="PLS90_13510"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK03460.1"
                     /translation="MRKRQLACAILLLLVCSVATALMIDDFDGAVWNPDGHWRPDNII
                     QNSGLLDTITFDQALDAEHLVVDYFNDGSGSQTTLVLRDDVSLPVGNWVQLDVAVTNG
                     PQSINIRAGLALASSLGRNAPAKGGIDRANTLLWGLRQDGLGRAHAYGAGGEEFGDAT
                     GPIAGYANGKWFTLAIRRADATRFELYQAPTGQPLQLIATINFSGSTGAGESPPTIPG
                     LLLDNGLTSFIALFDNFEIGDDWPPPIRDPRPIATPVPTPPPDVPDDEIALLLPTNIM
                     DLPRIESTRHTVHLPAGAADYTYHHNALLTHWRGRFYMAWTATPQSECTDPYEAWIAS
                     SPDGESWTDPINAGVASGGDPIYAQYMRDRFGLSQQSPLTVNAAPRGWNATPDKLYLW
                     SLSWTVSARGRDWVGRIWWTDDGETWHEIAPTELDRLEREDGLRVRGSASNKSFIPLS
                     DGRLMAAAMEGVFQNGKQLCTPITDDPSGLTGWTGGWIDTSDCPDVGEPNGWEGPDGV
                     LHYTVRFGTFVWHAFSNDGGQTWSKLRQQPFFTDNPGNKHFGTLPDGRIFYLGGPIPG
                     SRMQLVLGISADGWIFDQNYLVRWEQIQPIWYSQCKSEDRPGYEYPALLYHDGYLYAA
                     YSRTRDYIEVSKFKASQVLKNTVARRWGGYQ"
     gene            5731..>6458
                     /locus_tag="PLS90_13515"
     CDS             5731..>6458
                     /locus_tag="PLS90_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03461.1"
                     /translation="MKPFLTALIGYCVAASVAFADEPVQLQWLLNFDGAGADAAAKYY
                     EPDPTVGERPPNMIQPGDLIPDPRVRPYYGSWDSGGIHILNNSGKLGFVTLADGRKVL
                     ELSGAEEAGDGLVFEHFLEGQPSRVLEAIVSVGEAPREQLARIISSNDWRMRPPGQIV
                     ALGIDQLGHAAVELRAPNAFDFYPVAEELDSMHRFVRDTRSIAADGQFHHVAVVYDHV
                     QQTIYLYVDGKLARATPMQGDDHE"
BASE COUNT         1103 a         1949 c         2257 g         1149 t
ORIGIN      
        1 aagcggtacg tgagctgggt tcagaacgtc gtgagacagt tcggtcccta tctgatacgg
       61 gcgcaggagt tttgcgtgga tctgtcccta gtacgagagg accgggatgg acggaccgct
      121 ggtgttccgg ttgtgccgcc aggtgcagcg ccgggtagct atgtccggag ccgataaccg
      181 ctgaaagcat ctaagcggga aacggaccac aagataagaa ctcccgggcc gcaaggcccc
      241 tgaagacccc tcggagacta cgaggttgat aggcggaagg tggaagcatg gcaacatgtg
      301 tagctgatcc gtactaatcg gtcgtgcggc ttaaccctac tgcttttctt tcctcccggc
      361 tgaagactct tcgctccgca cccgcttcgt tctcactcgc tgtcaaacag acgtccgccg
      421 cgcgcggccc caccccccgc gcgcgcaagg tttccggtgg tcatagcgag agggcaccac
      481 ccgttcccat tccgaacacg gccgttaagc ctcttcgcgc cgatggtact tggctcagtg
      541 ggcctgggag agtaggtcgc cgccgggatt tttccgcccg cccgcatcgt tccgcgcgat
      601 gcgggcggtt tgctttttcc ccctcccctc gcccggcgtc acgcatcggt cgatcctcag
      661 gacggagatt acagagaaag agctcggagc gcggcgttga tttttcttcc ggagacttgc
      721 gatcagcgtt cgaagcgggg cgccggccgg acggggcccg gggcgatgcg gtcgacggcg
      781 gcgagcgtat cggccgtcag ctcgcgcgcg atccggtcgg cttccagggc cgaaatcaga
      841 tcccgatccg cccgcgcgcc gacgatcacc gaactgacgg ccggctgcgc caggtcccac
      901 gccagggcga actgggccag cgtgacgccg tagccctcgg ccagttccat cagggcctcg
      961 acctggtcga gaacggcatc gttcatttcg ggttcccagc cggcgatggc gcggtcctca
     1021 tttccggcca tgcccacgcg gcggtagcgc ccgctgagga tgcccgcctt gagcggctga
     1081 tagggaatga ccccgatctg gttcttctcg tagaacggca ggtcgttctc gtatcggcga
     1141 tcgaggatgc tgagggggac ttccgagacg cccgccatcg gccagccgcg gcggtcggcc
     1201 ttccagagat actcgcaaac ctcccagccc tgatgattcg agatgccgaa gtagcgcgcc
     1261 ttgcccgagc tgacgatgcg gtcgatcgcg cgcagcacct cctcggtgtc ggcgaacgga
     1321 tcgggccagt ggatcatgaa gatgtcgacg tagtcggtcc ggaggcggcg caggctgttg
     1381 tcgatctcgc gttggagatg ggtggaggag agcccgcgat gctgcggtcc gggccgcagc
     1441 gggacggcgg ccttggtcag cagcacaacg tcatcgcgca ggccttcgag ggccttgccg
     1501 agcatggact cggaagtgcc cagaacaatc tcgccgcgat tggggccgtc gtagcagttg
     1561 gcggtgtcga tgaaattgat accgtggtcg agcgcgaggc gcaccatgtg gtcgacgtcg
     1621 gcttgctcgg tgggggggcc gtaacccatc gtgcccaggc agatgggtga gacggcgagt
     1681 ccggtcctgc caagtcggcg atatttcatc ggataaaagc cccttcagcg gcggtgtata
     1741 gggttggaaa ttgttgcggg gtggcgttga agccgggcga cagtattgga atttggttta
     1801 cgaaagtcaa gctatatgcc agacaaatag cagacggatg cgactccagt tggcgtcggg
     1861 ggacgagggg gtcgaagggc gggaacggtc gaggacgagg acgagggtcg attcttgagt
     1921 gagagacaga tgagcgtttt ggttcttgcc gatgacctga ccggattggc cgaagtcgcc
     1981 tgccaggcgg cgcggtatgg cctgggcgga ccgatggcgc tgacgccggc ggcgccgcgg
     2041 ggtcaaccgg atacggaaaa caacagtcga ctgttcgtca atctgggttg ccgggcgctg
     2101 ccgccagccg aggccggcga gcggatccgc gatgcgctgg cgggcccatt gaaatcggac
     2161 ggcggcgacc gcgtttttct gaaaatcgac tccacggcgc gcggcccgat ccccgcgatg
     2221 ctggccgcgc tgcatgccgc ctgcccggag acgacgatcc cgctctgtct cgtcaatccg
     2281 gccacggggc gctcggtgcg ggaggggcgg ctccatgtca aaggtgatcc ggtagaccgg
     2341 acggagtttg cccgcgatcc gctgcatccg gtcaccgacg cctgcctgcc gcgatggctt
     2401 gcgcatccgg aactacgcgt ggaacgcgag tcgcgctcgc catcgggggg acgggacgtc
     2461 gccatcctcg acggcgaaac cgatgccgac ctggccgccg ccacccgccg ctgggcggat
     2521 tcaccggtgg cggcggggac ggcggcgttc gggctggcat tgctgcgcag ctggtcggga
     2581 tcgcccgcgc ccgcgcccgc cccgcagtgg acggttccac ccaacacgct ggtggtggtg
     2641 gggacgaaat caccctcggg ccgggcgctg atcgaggact tcgggcaaac cggccgccgc
     2701 gccgcaccgg cgagtgaagg ggtccagacc gccgcgccac gcatcctgca ctcgcccgcg
     2761 gaagacgcct cacccgaagc ggtgctggcg aaactcgtcc ggcggaccga ggaggccgtc
     2821 agccgccggc gaccggatct gctgatcatc ctcggcggcg agacggcgct ggcgatcatc
     2881 cgcgcgctcc ggctcgactg ggcccggccg gtcgcctacc gcgacggcct gggctggatc
     2941 gaagcgcggc atgagggccg gccgctgccg atctggctga tgccgggcgc ctactatcgc
     3001 cgcgggctgt ggcgggagct tgccacgcgc tgaatacttg tcacgggccg gattcagacc
     3061 tgttccggcc agcccttgca ggggtttggt tcgtcggggc ggcgacgcat ggttccggcc
     3121 cacggccgtc acccgcgctc tattccggcc gtccgccccg cggactgacc gagcaacaag
     3181 tcgaatcgcc tgcactgaac ctggattcct gaagcagctt gcgatatggg gaatgccgcg
     3241 cgctgagctt cgggcctact gatatccgcc ccatctgcga gcgacggtat ttttcagcac
     3301 ctgcgaagct ttgaacttcg acacctcgat gtaatcgcgc gtgcggctgt aggcggcata
     3361 gagatagccg tcgtgataca gcagggccgg gtattcgtag ccgggccgat cctcgctctt
     3421 gcactgtgaa taccagatcg gctggatctg ctcccagcgg accaggtagt tctggtcgaa
     3481 gatccagccg tcggcgctga tgccgagaac gagttgcatc cggctgccgg gaatgggtcc
     3541 gccgaggtag aagatgcggc cgtcggggag cgtgccgaag tgcttgttac cggggttgtc
     3601 ggtgaagaag ggctgctgtc tgagcttgct ccacgtctgg ccgccgtcgt tgctgaaggc
     3661 atgccacacg aacgtgccaa accgaacggt gtagtggagg acgccgtcgg ggccttccca
     3721 gccgttgggc tcgccgacgt cgggacagtc gctcgtgtcg atccagccgc cagtccagcc
     3781 ggtcagaccg gagggatcgt cggtgatggg agtgcaaagc tgtttgccgt tctgaaagac
     3841 gccctccatg gcggccgcca tcagccgacc atcgctgagg gggatgaagc tcttgttgct
     3901 ggcggaaccc cggacgcgga gtccatcctc gcgctcgagc cggtcgagtt cggtcggcgc
     3961 gatttcgtgc caggtttcgc cgtcgtcggt ccaccagatg cggccgaccc agtcgcgtcc
     4021 gcgggcgctg acggtccatg acagcgacca gagatagagc ttgtcggggg tggcgttcca
     4081 gccgcggggg gcggcattaa ccgtcaacgg gctctgttgc gacaggccga aacggtcgcg
     4141 catgtattgc gcgtagatcg gatcgccgcc cgaagcgacg ccggcgttaa tcggatcggt
     4201 ccaggattca ccatccggcg acgaggcaat ccacgcctcg taggggtcgg tgcactcgct
     4261 ctggggcgtg gccgtccaag ccatgtagaa cctgccgcgc caatgtgtga gcagcgcgtt
     4321 gtggtggtag gtatagtcgg cggcgccggc gggcagatgc acggtgtggc gggtgctctc
     4381 gatgcgcggg aggtccatga tgttggtcgg cagcaggagg gcgatctcgt cgtcggggac
     4441 gtcgggcggg ggggtcggca ccggcgtggc gatcggtcgc gggtcgcgga tgggcggagg
     4501 ccagtcgtcg ccgatctcaa aattgtcgaa gagggcgatg aaggaggtca ggccgttgtc
     4561 gagcagaagg ccggggatgg tgggcgggga ttcgccggcg ccggtgcttc cgctgaagtt
     4621 gatcgtggcg atgagctgga ggggctggcc ggtcggggcc tgatacagct cgaatcgcgt
     4681 ggcgtcggcg cggcgaatgg ccaaggtgaa ccacttaccg ttggcatagc cggcaatcgg
     4741 gccggtggcg tcgccgaatt cctcgccgcc ggcgccatag gcgtgggcgc gccccagccc
     4801 atcctggcgc agcccccaca gcagcgtgtt ggcgcggtcg atgccccctt tggccggggc
     4861 attgcggccg aggctggacg ccagcgcgag gccggcgcgg atgttgatcg attgggggcc
     4921 gttggtgacc gcgacatcga gttggaccca gttgcccacc ggcagcgaca cgtcgtcgcg
     4981 cagcacgagc gtcgtctggc ttccgctgcc gtcgttgaag tagtcgacga ccagatgctc
     5041 ggcgtcgagc gcttgatcga aggtgatggt gtcgagcaag ccgctgttct gaatgatgtt
     5101 gtcgggccgc cagtggccgt cgggattcca gacggcgccg tcaaagtcat cgatcatcaa
     5161 ggccgtggcc actgagcaga ccagcagcag gaggatggcg caagccagtt gccgtttgcg
     5221 catcggagag ctccttgggt ttgatggtcg atggaacggc gatggggttc gacggagagc
     5281 accgatgcca ccagagtatg tcatatgata tatggcgtca agccgatcac atcggaggaa
     5341 aggccgtggg agagaggaag cgtggcgcgg cgggcggagg acccaacgga acattaaacg
     5401 cgcgcggaga cacggagacg cggagcaacc gggatcggga tcgaaaagag agaagttcag
     5461 agtaacgtca tctttatcgg ccgatctgcc gcataatgtc tgatttttgt tgaccgcgcc
     5521 gttttaaccc tgcataacgc tctcggaatg taagatcgct cgaatcgtag aacttgatct
     5581 ccatttgacc acgttcaggg agatttccgg ggaaccgagt cccgcgtcag gttcaaagcg
     5641 aaccaacacc cggcgggcgt ccccatgcat gaggacgaac ccgtccgcgc cgttccgcgc
     5701 gatcgaaaac tcaaggaagt tgtgaggcag atgaaacctt ttctgacggc cctgattggt
     5761 tactgcgtcg ccgcgagcgt ggcgttcgcc gacgaacccg tccaactgca atggctgctc
     5821 aactttgacg gggcgggagc cgatgccgcc gccaagtatt acgagcccga tccgacggtt
     5881 ggcgaacgcc ccccgaatat gattcagccg ggcgacctga tcccggatcc ccgggtccgg
     5941 ccgtactacg gcagctggga ttcgggcgga attcacatcc tcaacaacag cggcaagctc
     6001 ggtttcgtca cgctggccga tggccggaag gtgctggagc tgtcgggcgc ggaagaggcg
     6061 ggcgacgggc tcgtgtttga gcattttttg gaggggcaac cctcgcgcgt cctggaggcg
     6121 attgtctcgg tcggtgaggc cccgcgggaa cagctcgccc ggatcatcag ttccaacgac
     6181 tggcgcatgc gccccccggg gcagattgtt gccctgggga tcgaccagct gggtcatgcg
     6241 gccgttgaac tgcgcgcgcc caacgccttc gacttctatc ccgtagccga ggaactggat
     6301 tcgatgcacc gcttcgtgcg cgacacgcgg agcattgcgg ccgacggcca gtttcaccac
     6361 gttgccgtcg tctacgacca cgttcaacaa accatctatc tgtatgtgga tggcaagctc
     6421 gcgcgcgcga cgccgatgca gggggacgat catgaatt
//
LOCUS       DAOPCO010000230         6457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10582_length_6457_cov_5.059044, whole genome shotgun sequence.
ACCESSION   DAOPCO010000230 DAOPCO010000000
VERSION     DAOPCO010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10582_length_6457_cov_5.059044"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            459..1874
                     /locus_tag="PLS90_13520"
     CDS             459..1874
                     /locus_tag="PLS90_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPK03462.1"
                     /translation="MALSVNTNVASLNAQRNLMINSAGLGKSLQRLSSGLRINQASDD
                     AAGLAISNGLTAQVRGLNQAVRNAGDGLSLLGTAESAISEQTNILQRIRELAVQAAND
                     TNSTTNRSSLNDEVDALVSELSRIAGTVEFNGTKLLDGTFTAKELQVGASAGTNQKIS
                     IDISGTRATDIGSPYEIAGTAVTTGGFTEGQVKITVNGQSFFAAATADDGVSSTDQGG
                     SALAYATAINAVSGSTGVKASASTSVTGAAQQVANHDTVDATNFLKINGVDVGTFNVT
                     AADSTAVIDAINAKFSETGVKASLGDDDKITLTAEDGRNIEVAAGGDAATASGLSSGT
                     TRGTVTLSSTQEFTVADSSGNLNADGTAAKNTGKNVNTIDISTKSGAEQAIEIVDVAL
                     ENLNSRRAGIGALVSRLNSVISNLSAISENVAASNSRILDADFAAETANLTRTQILQQ
                     ASVAVLAQANQAPQLALSLLR"
     gene            2018..2440
                     /locus_tag="PLS90_13525"
     CDS             2018..2440
                     /locus_tag="PLS90_13525"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HPK03463.1"
                     /translation="MAIPVVSLAYGSMGLGIRDVAAPSSASGNSQKSPRQTDALLPTE
                     LAPVGQGASKQDVSGTGTEQTRLVDDIRQTAEKINEYLRIADTHLEFSVSEQTGRIVV
                     HVIDSETREIVREIPPETMNRFAKRMTLMRGLLFEASG"
     gene            complement(2650..3789)
                     /locus_tag="PLS90_13530"
     CDS             complement(2650..3789)
                     /locus_tag="PLS90_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016858626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HPK03464.1"
                     /translation="MRFATKTIRAGQPPDPVTGAVIVPIHPAVTFQFQEVGVPRAFEY
                     SRTANPTRAALEQCLAQLEGAAHGLAFASGMAACDAVLSILDPGDHVVAAGQIYGGTF
                     RLLQTEYARRGVTVTYVPADDPAAFARAIQPATRLVWTETPTNPLLQLTDIAAVAAAA
                     RRHAIPLVVDNTFPSPYFQRPLELGADVVIHSTTKYLGGHSDVLGGAVITNDPELHAR
                     FQFHQNNVGAVLGPFDSWLTLRGIKTLVVRMRQHEANARRVAEFLERHPRVQRTIYPG
                     LPSHPQHDLARRQMSGFGAMVSFELDGGRQAANAFVKRLRLFLFAESLGGVESLVCHP
                     VTMSHAALTERERAEAGITEGLLRLSVGIEDGDDLLEDLEHALAD"
     gene            4391..5263
                     /locus_tag="PLS90_13535"
     CDS             4391..5263
                     /locus_tag="PLS90_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03465.1"
                     /translation="MSLQFSIARMCCIALFVALLAPGSHAIIWINEDFEDGIAFDHAP
                     GTATGGAVDYYFEGGTDVVTTTSVAVTGTVSTHRALSGSRSLRLGAGEGFAVDHGVES
                     PESGPVQIFQMAVSLDQVPAAAGEKFAEWRVPWSRADDPGTPADETLNYTFYMKFISD
                     GANGVLVIAGQEGDNPTPATGIGRTTANEVKWIMLTFVYNRSGAPQEDPHLGTIPPGA
                     HFYGSSVSSGLSFATEPGGGTGWPSFEVHDGVVYVDDVYWEGGLNLDHEPSLAARNLK
                     AVDVPVLLNHFDLE"
     gene            complement(5400..6119)
                     /gene="queC"
                     /locus_tag="PLS90_13540"
     CDS             complement(5400..6119)
                     /gene="queC"
                     /locus_tag="PLS90_13540"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPK03466.1"
                     /translation="MRSQPQPQPKAVVLLSGGLDSTTALAIARAEGFAPHALTVHYGQ
                     RHARELDSAARVARAFGVAQHVTSTVDLRLFGGSALTAEIDVPRGRSPDAIAAGIPIT
                     YVPARNTIFLSLALGYAESLGALDIFIGVNAIDYSGYPDCRPEFIESFERTANLATRA
                     GVEGPRPIRIHAPLIHMTKAQIIRRGIELGVDYALTTSCYDPAPDATACGECDACLLR
                     LKGFAEAGLPDPIRYQRPPLL"
BASE COUNT         1187 a         1826 c         2447 g          997 t
ORIGIN      
        1 tatggcggtg tgtggcgggg ggggaacggg ggaggaggga cgaggggcgg gcggtgtgta
       61 ataaaaaaaa ggacagtgcg ggagaaattt gatgacaggg ggggcgggga cgatgaggga
      121 gtcagaagcg agccaagtgc ttagctgacg ggcgtccagg agggacaccg tcgcgagacc
      181 tcagccgcat gacgcgagaa cgcatgttgg gtccgccgga ccggggtgcc ccgtcggtcg
      241 aggcaggaca aaggaggttc gccgcaggga gataggaaaa ggggcgatcc gcgcctggac
      301 gtcgcgcgga aaaccaggac ggttagacag ccaaggcgac cggccctcct tcgcaaatac
      361 atgaaggggg tgcaattctt accctgatcg tctgccatcg ggcagggacg tccgatggaa
      421 cgattcgcca agatggttct ttcacggagg aaagagcaat ggcattgagt gtcaatacca
      481 acgttgccag cctaaacgcg cagcgcaacc tgatgatcaa ctcggcgggc ctgggcaagt
      541 cgctgcagcg gctgtcgtcg ggtctgcgga tcaaccaggc gtcggacgac gcggccggtc
      601 tggcgatctc caacggcttg acggcgcagg tgcgcggtct gaaccaggcc gtccgcaacg
      661 ccggcgacgg gctgtcgttg ctggggacag cggagagcgc gatttcggag cagaccaaca
      721 tcctgcagcg gatccgcgag ctggcggtgc aggcggccaa cgatacgaac agcacgacga
      781 accgctcgtc gctgaatgac gaagtcgacg cgctggtgtc ggagctgtcg cggatcgcgg
      841 ggacggtgga gttcaacggg acgaagctgc tcgacggcac gttcacggcc aaggagctcc
      901 aggtgggcgc ctcggcgggg acgaaccaga agatctcgat cgacatcagc gggacgcggg
      961 cgaccgacat cggcagcccg tacgagatcg ccggcacggc ggtcaccacg gggggcttca
     1021 ccgagggcca ggtcaagatc acggtcaacg gccagagctt cttcgcggcg gcgacggccg
     1081 acgacggcgt ctcctcgacc gaccaggggg gctcggcgct ggcctacgcc acggcgatca
     1141 acgcggtgag cggctcgacc ggcgtcaagg cttcggcttc gaccagcgtc accggggcgg
     1201 cgcagcaggt ggccaaccac gacacggtcg acgcgaccaa cttcctgaag atcaacgggg
     1261 tcgacgtcgg cacgttcaac gtgacggcgg ccgattcgac ggcggtgatc gacgcgatca
     1321 acgccaaatt cagcgagacc ggcgtgaagg cctcgctcgg cgacgacgac aagatcacgc
     1381 tgacggccga agacggccgg aacatcgaag tggcggccgg cggcgacgcg gcgacggcct
     1441 cgggcctgag ctcggggacg acgcgcggca ccgtgacgct gtccagcacg caggaattca
     1501 ccgtggccga cagctcgggg aacctgaacg cggacgggac ggcggccaag aacacgggca
     1561 agaacgtcaa cacgatcgac atttcgacga agtccggtgc ggaacaggcg atcgaaatcg
     1621 tcgacgtggc gcttgaaaac ctgaactccc ggcgtgccgg tatcggcgct ctggtcagcc
     1681 ggctcaactc ggtgatcagc aacctgagcg cgatcagtga aaacgtcgcg gcgtcgaatt
     1741 cgcgcatcct ggatgcggac ttcgcggccg agacggccaa cctgacccgg acccagatcc
     1801 tgcagcaggc gtctgttgcg gttctggccc aggcgaacca ggcaccgcaa ctggctctct
     1861 cacttctccg gtaaggtaag gtccggcccg gtcctggccc aggcggccgg gatcgggccc
     1921 accgtgccgc gcgacgtggg actcgggttg accagcaacc gattaaggaa aggacaaacc
     1981 ccttaaccgg cggaacacca gatcgaggtg cagtacgatg gcgataccag tcgtctcgtt
     2041 ggcctacgga tccatggggc ttgggatccg tgacgtcgcc gcaccttcgt ctgcgtctgg
     2101 aaactcgcag aagagtcctc gccagacgga cgccctgcta ccgaccgaac ttgcaccggt
     2161 gggtcaagga gccagtaaac aggatgtttc aggcacaggg acagagcaaa cccgtcttgt
     2221 cgatgacatt cggcaaacgg cggagaagat caacgagtat ctgcggatcg ccgatacgca
     2281 cttggaattc tccgtgagtg aacagaccgg acggattgtg gttcacgtca ttgacagtga
     2341 aacgcgggaa atcgtccggg agatcccccc tgaaacgatg aatcgcttcg ccaagaggat
     2401 gaccctgatg cggggtctgc tcttcgaagc gagcgggtaa ctctcccggg attcgattcc
     2461 ccggttcgtg tcgcggccgg gggcgggcaa gcggccaggg gtatttcccc cggtcgccgc
     2521 cgccgcccgg ccaggcaccc cagacagaaa aactcccccg gcggagcgcc ccggtgacga
     2581 cctcaccggg cgccggccgg gggagttcct ttttggcggg tcgatgcacg ggcgggcctg
     2641 gatgccgtgc taatcggcca gggcgtgctc cagatcctcg agcaggtcgt cgccgtcctc
     2701 gatgccgacc gagagacgca gcaggccctc ggtgatgccg gcctcggcgc gctcgcgttc
     2761 ggtcagggcc gcatggctca tcgtgacggg atggcagacg agcgattcga cgccgcccag
     2821 gctctcggcg aagaggaaga gccggaggcg cttgacgaag gcgttggcgg cctgacggcc
     2881 gccgtcgagt tcaaagctga ccatggcgcc gaagccgctc atctggcggc gcgccaggtc
     2941 gtgctgcggg tgggagggca ggccggggta gatcgtgcgc tggacgcggg ggtggcgctc
     3001 aaggaactcg gccacgcgcc gggcgttggc ctcgtgctgg cgcatgcgga cgaccagcgt
     3061 cttgattccg cgcagcgtga gccaggaatc gaacggcccg aggaccgcgc cgacgttgtt
     3121 ctggtggaac tggaagcggg catggagttc ggggtcattg gtgatgacgg cgccgccgag
     3181 gacgtcggag tggccgccca ggtatttggt cgtgctgtgg atgacgacgt cggcgcccag
     3241 ttcgagcggg cgctggaaat aaggggaggg gaaggtgttg tcgacgacga gcgggatggc
     3301 gtggcggcgg gcggcggcgg cgacggcggc gatgtccgtg agctggagga gggggttggt
     3361 cggggtttcg gtccagacca gcctggttgc gggctggatg gcgcgggcga acgccgcggg
     3421 gtcatcggcg ggcacgtagg tgaccgtgac gccgcgccgg gcatattcgg tttgcaggag
     3481 gcggaaggtg ccgccgtaaa tctggccggc ggcgacgacg tggtcaccgg ggtcgaggat
     3541 cgagaggacg gcgtcgcagg cggccatgcc ggaggcgaag gcgaggccgt gggcggcgcc
     3601 ctcgagttgg gccaggcatt gttcgagggc cgcgcgggtg gggttggcgg tgcggctgta
     3661 ttcgaaggcg cgcgggacgc cgacctcctg gaactggaaa gtgacggcgg gatggatcgg
     3721 gacgatgacg gcgccggtga cggggtcggg gggttgaccg gcgcggatgg tcttggtggc
     3781 gaagcgcatg gggtcagttc ctttccgctc agcagaggcg gaccatgata gcagtttggg
     3841 gggcgggagt taagggcttg tcattcggca attgagaccg gctccggccg ccccagccag
     3901 gggcttgatt tgttgggggc ggcggcgcag ggttccggcc cggggccgtc accctgcgct
     3961 ttgatccggt cgtccgaatc cggtccacaa gtcagaaccg ccggcattga agatgggatc
     4021 ttcaagcagc ttgggatttg gcgacgcgtt gcgctgcaag gcaatgccgc cgcgccaggg
     4081 cagtgctccc ggctcaggtc aggcggaaac ggatcggccc ggcgcgggcc ggagtgcggg
     4141 cgtttcggtt aaaaaaccga ggcataagcc ataaactaaa cctttttgat aaattaccaa
     4201 catatcatca tactgatttt ctgaattgca atatggcatg gggcggacta tccttgcaag
     4261 catgagtctc cgccgagcgg gaaacgccgc gctccctggc gccggggggg cggtcgcccg
     4321 cgcgttgagg agtcccctga tcgacgttga agggctggaa ggcttaatcc caagggaagg
     4381 agaaccaacg atgagcttgc aattcagcat tgcacgcatg tgctgcatcg cgctgttcgt
     4441 tgccctactc gcccccgggt cacatgccat catctggatc aacgaggatt tcgaggacgg
     4501 aatcgcgttt gatcatgcgc caggcacggc aacgggcggg gcggtggatt actattttga
     4561 aggcgggacc gacgtggtca cgacgacatc ggtcgcggtg accggaacgg tgtcgaccca
     4621 ccgcgcgctg agcggcagcc gctcgctgcg gctgggcgcg ggcgagggct tcgcggtcga
     4681 ccacggggtc gaaagccccg aaagcggccc ggtgcagatc ttccagatgg cggtcagtct
     4741 ggatcaggtg ccggccgcgg cgggggagaa gttcgccgaa tggcgggtgc cgtggagccg
     4801 ggcggacgat cccgggacgc ccgccgacga gacgctcaac tacaccttct acatgaagtt
     4861 catctccgac ggcgcgaacg gcgtgctggt gatcgccggc caggaggggg acaatccgac
     4921 gcccgcgacg gggatcggac ggacgacggc gaacgaagtg aagtggatca tgctgacgtt
     4981 cgtctacaac cgcagcgggg cgccgcaaga agacccacac ctggggacga ttccgccggg
     5041 ggcgcacttc tatgggtcga gcgtcagttc ggggttgtcg ttcgccacgg agccgggcgg
     5101 cggcacgggg tggccttcct tcgaggtgca tgacggcgtg gtgtatgtgg acgacgtcta
     5161 ctgggagggg gggctgaacc tggaccatga gccgtcgctg gcggcgcgca atttgaaggc
     5221 ggtggacgtt ccggtgctgc tcaatcactt cgacctggag tgagagagaa cggatgggcg
     5281 gggcggcttg tctggcgccg ccaagccgcc ccggatggat ggccggcggt cgaaaggtcg
     5341 ccggccgttg tctattttgg gggtggacga gggacgagga cgaggacgag agacgattct
     5401 tagagtaaag gggggcgctg atagcggatg gggtcgggca ggccggcttc ggcgaagccc
     5461 ttgaggcgga gcaggcaggc atcgcactcg ccgcaggccg ttgcgtcggg cgcggggtcg
     5521 tagcacgagg tcgtcagggc gtaatcgacg ccgagctcga tgccgcggcg gatgatctgc
     5581 gccttggtca tgtggatcag cggggcgtgg atgcggatcg gccgggggcc ctccacgccg
     5641 gcgcgcgtgg cgaggttcgc cgttcgttcg aaggattcaa tgaattcggg gcggcagtcg
     5701 ggatagccgc tgtaatcgat ggcgttgacg ccgatgaaga tgtcgagcgc gccgagggac
     5761 tcggcgtagc cgagcgccag cgagaggaag atcgtgttgc gcgcggggac ataggtgatg
     5821 gggatgccgg cggcgatggc gtcgggggag cggccgcgcg ggacgtcgat ctccgcggtc
     5881 agggcggagc cgccgaaaag gcgcaggtca accgtggagg tgacatgctg ggcgacgccg
     5941 aaggcgcgcg cgacgcgcgc cgccgagtcc agttcgcggg cgtggcgctg gccgtagtgg
     6001 acggtcaggg cgtggggcgc gaacccctcg gcccgggcga tggcaagggc ggtggtggaa
     6061 tcgagtccgc cgctgagcag gacgacggcc ttgggttggg gttggggctg cgagcgcatg
     6121 agatccggcg cctgtggtcc gcgttcggag aattctcgcc ttgagttgta cgcgatcgag
     6181 cggccgatgg catcaggaaa gcgccggggg ggagggcggg gagcggagag cggtttgccg
     6241 gcttatcggg ggaaaccatt gaaaacggtt gaggaaattt tgtcggaacc ggtcgccggg
     6301 ccgaggcccg gcgctagaat cagtgcctac gcgggtgcct atgcaaagcc cgctatcgcg
     6361 gctcgcgggg cggaccggcg ggggcgacgg tcccacacat ccccccttaa tccttcataa
     6421 cggctcagag ggtggcgcat aaaaaagaag aaccatc
//
LOCUS       DAOPCO010000231         6401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10680_length_6401_cov_3.254176, whole genome shotgun sequence.
ACCESSION   DAOPCO010000231 DAOPCO010000000
VERSION     DAOPCO010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10680_length_6401_cov_3.254176"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..2031
                     /locus_tag="PLS90_13545"
     CDS             157..2031
                     /locus_tag="PLS90_13545"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPK03467.1"
                     /translation="MKLRCLGILLVGLILAHGARARDWTYIPSPQALPGFPPPAWSGY
                     GPNNSTSALYMTESAVGLGSRDATITGQAAICYVPDGSALPAAVEYDLGDVYNLYELY
                     YWTPNYQGYQTSVMKNVEIAYKVNAGDSYTVLKTVTMAKGAAAPDVEVGPTNQDGTGD
                     PVDLENVQARYLRLTYLDNWGGPYHEAGKFRVYQAGQPLDQVLTLANYGPAGKTGGIQ
                     SIDASALDGTAFSLVNPPSLPASLNSGQSLALTIRFNGADPNTPYTADLPIEYTDQYG
                     THLATVHLTGQTEDTPVAFALTPATHDFGTVQVGQTQSILIELTNDENAAAAATVDRI
                     EISGGGQLLTISAKPGLPLVLDPGEIAEVEVQLHAHVSGLGPLVASLEADFDDGVDAR
                     TLSCDFTALIAGPEPTFIPNVAATASTYYYETENYHPKWLVETEGEVGVTEVPAYALG
                     SKQMLHSSAIYLAQWYSTYLDPWEIQWVEFDLQQVYSLGQMWVWNLNFPGTGYFSAES
                     GMKDVHVLYKTAVDDAWSTLTASGGGDVFTLAKAAGADDQPATNLHDPDLPDTWGDPV
                     PFGGLQARYVRIQQASVAPGEGNWGGGGYSYAGLAHVRFYQSAPPPNAVGTGAWAIYR
                     "
     gene            2137..3105
                     /locus_tag="PLS90_13550"
     CDS             2137..3105
                     /locus_tag="PLS90_13550"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK03468.1"
                     /translation="MMQKRPMRNSLAATQRHERLSGVIPALPTPLDAHEDVDLNALRR
                     LLDHCVGSGAAAIFLLGSMGEGPNLVNEQKRLAVRAAVRHLDHAVPLLANISETSTRR
                     TLELGRLIQDEQPDYLISTGPFFYHYPNPDSQLEFFARLAEELALPLIFYHQPGMTGN
                     ACDVDTIDQIVNMPRVAALKDSSCNFGLIAVLLRRYPDRATRPFSLLQGDESLFDITL
                     LLGADGVISGGGTAFIPLLLELYRAASAGDRERAYALNAQFRAALLEMLGPNLKVDWM
                     AAVKDTLKQRDLCENICTSPFVNRFRVLDGHGQTALLRPAAAGRPG"
     gene            3116..3889
                     /locus_tag="PLS90_13555"
     CDS             3116..3889
                     /locus_tag="PLS90_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03469.1"
                     /translation="MHLADLLSLKGKVCLVTGGAGLYGRHITEALAEAGGTVVTASRS
                     LEAGQHLADELARRGLAVAALTVDQADHESVMQLKAELEQRHGRLDVFINNAVARPMK
                     RYEDPLAAWAESMRVNATGMFDITREMADLIARGGGGSIVNIASMQGVYAPDFMLYEG
                     TAMDSPPDYHFHKGGLITLTRYLARKLATRNIRVNAVSPGGLLGDQDPQFLGRYNQKV
                     PLGRMALPDDIKGVVVFLASPASAYITGENILMDGGLHA"
     gene            3923..5122
                     /locus_tag="PLS90_13560"
     CDS             3923..5122
                     /locus_tag="PLS90_13560"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK03470.1"
                     /translation="MGSLAWRFDQRERIYINEVLDSGFASSTTGTMNQRLEAAFAARF
                     GRKYAVTCNSGTTTLHAALVAIGVGFGDEVITTPLTVISCMNAIVYCNAIPVFADVDP
                     NTFLLDPDDVERKITPRTRAIMPVHLYGQVCDMTRIMEIAQRHDLAVLEDCAQCYLGT
                     HRGRIGGTFGNAGSWSFENSKHLTTGDGGILACDSEELGTRLRKLCCQGFRTCTAVSG
                     QVRLSKDDFQNPNYKRHDSFGFMYRLPEVAAAMGLAQLEKIDWFVAKREQMARLYAEA
                     IQGVDWLVPQQTPAGDRNSYYTFALRFLKKDVSWEAFRKKHIEYGGDGAYAAWTLCYL
                     EDSVPEILALFRRMGLDRRMATDRGICPHAEMIQRELMQFTTNQKDEDEMKVQAEALY
                     RTVRYFS"
     gene            5287..>6401
                     /locus_tag="PLS90_13565"
     CDS             5287..>6401
                     /locus_tag="PLS90_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03471.1"
                     /translation="MKWRTRAGHLVLLIAMFAWDSAARAADGSDWVLPHRFRVAIDCD
                     TGDPASTNSLACLELDLNRLLSDNSISGVVSYSSLRLMRIGAHQELSTNTIQTELPFA
                     LTGDTRSYGPVTLWWRAPDASARSYYLYFETEFRAAVAGEAAPFPTSGPIGIGDTFHY
                     HDGQPGPADCVPLHSTFWPLDWDGDGVFDLIGIAFRIYEHGEDLEENLGNGVYFLKNI
                     GSNENPLFAPRLRMKDRNGAWLRTDQLGQNMHPMDWDGDGDMDFAGVAGNTLLLWENT
                     GSRDAHGLLMLNQPVPIMDLPVSQFRAEMPGVVRTPSFTCKGLRRVDWEGDGDLDLIV
                     AWACVNLLKEVDPTTGVMPYGAALMHFELYERVGDDQG"
BASE COUNT         1155 a         2199 c         1872 g         1175 t
ORIGIN      
        1 cacggggcgc cgctggccgc gcgcgccccg cgcagcatgc cgcgccgcct tccctgccgg
       61 ctcggaagac acgttccatc gcaccggccc gccgcgccgc ggcccagtcc cccggcccgg
      121 cgggcccgcc aatccccgtg acaaggagga cctccagtga aactccgttg ccttggcatt
      181 ttgctcgtcg ggctcatcct ggcgcacggc gcgcgcgcgc gcgattggac gtatatcccc
      241 tcgccgcagg ctttgcccgg cttccccccg ccggcctggt cgggctacgg ccccaacaac
      301 agcaccagcg cgctctacat gaccgaatcg gcggtggggc tcggctcgcg cgacgccacc
      361 atcaccggcc aggccgccat ctgctatgtc cccgatggca gcgccctgcc cgccgccgtc
      421 gaatacgacc tcggcgacgt ctacaacctg tatgagctgt attactggac cccgaactat
      481 cagggctacc agacctccgt catgaagaac gtcgagatcg cctacaaggt caacgccggc
      541 gacagctaca ccgtcctcaa gacggtcacg atggccaagg gcgccgccgc tccggacgtg
      601 gaggtcggtc cgaccaatca ggatggcacg ggcgaccccg tcgacctgga aaatgtgcag
      661 gcccgctatc tgcgcctgac ctacctcgac aactggggcg gcccgtacca cgaggccggc
      721 aaattccgcg tctaccaggc cggccagccc ctcgatcagg tgttgaccct ggccaactat
      781 ggcccggcgg gcaagaccgg cggcatccag tccatcgacg ccagcgcgct ggacggcacg
      841 gcgttcagcc tcgtcaaccc cccttccctg cccgcctcgc tcaactccgg gcagagtctt
      901 gcgctgacga tccgcttcaa cggcgcggac cccaacacgc cctacaccgc cgatttgcca
      961 atcgagtata cggaccagta cggcacgcat ctggccaccg ttcacctgac cggccagacc
     1021 gaggacacgc ccgtcgcctt tgcgctgacg cccgcgacgc acgacttcgg cacggtccag
     1081 gtcggccaga cccaatcgat cctgatcgaa ttgactaatg acgaaaacgc cgccgcggcg
     1141 gcgaccgtcg atcggatcga gatcagcggc ggcggccagt tgctcaccat ctcggccaag
     1201 ccgggcctgc cgctggtcct cgatccgggc gagatcgccg aggtcgaggt gcagctgcac
     1261 gcccatgtct ccggattggg acccctggtg gcctcgctcg aggcggattt cgatgacggc
     1321 gttgatgccc gcacgctcag ctgcgatttc accgcgctga tcgccggacc cgagcccacc
     1381 ttcatcccga acgtcgccgc caccgccagc acctattact acgaaaccga aaactatcat
     1441 cccaagtggt tggtcgaaac cgagggtgag gtcggcgtca cggaggtccc ggcttacgcg
     1501 ctcggctcca agcagatgct gcacagctcc gccatctact tagcccagtg gtattccacc
     1561 tacctcgatc catgggaaat ccaatgggtg gagtttgatt tgcagcaggt ttattccctc
     1621 ggccaaatgt gggtctggaa cctgaacttc cccggcactg gctatttcag tgcggaatcc
     1681 ggcatgaagg atgtccacgt gctctacaag accgcggtcg acgatgcgtg gtccacactg
     1741 accgcctccg gcggcggcga cgtcttcacc ctggccaagg cggccggcgc cgatgatcaa
     1801 cccgcaacca acctgcatga ccccgatctg cccgatacat ggggcgatcc ggtcccgttt
     1861 ggcggcctgc aggcgcgcta tgttcgcatc cagcaggctt ccgtcgcccc gggcgaaggc
     1921 aactggggag gcggcggcta cagctatgcg gggctggccc atgtccggtt ttaccagtcc
     1981 gctccgccgc ccaacgcggt cggtacgggc gcctgggcca tttatcgatg agcggcgccc
     2041 cgtcgaccga ctcggcccca tccacgcgct cgcgggccat cggcgatcga ggccgaccgc
     2101 ccttcgcccc acgtatcccc tccggttgaa aggttcatga tgcagaaacg cccgatgcgc
     2161 aattccctgg cggcgacgca acggcacgaa aggctcagcg gcgtcatccc cgcgctgccg
     2221 acaccactgg atgcccatga agacgtggat ctgaatgccc tccgccgcct gctcgatcat
     2281 tgcgtcggat cgggcgccgc ggccatcttc ctgctcggca gcatgggcga gggccccaat
     2341 cttgtcaacg aacagaagcg gctggccgtc cgcgccgccg tccggcatct ggatcacgcc
     2401 gtgcccctgc tggcgaacat cagcgaaacc tccacgcgcc gcacgctcga actgggccgt
     2461 ctgattcagg acgaacagcc cgactatctc atctcgaccg ggcccttctt ctatcactat
     2521 ccgaaccccg acagtcagct tgagttcttc gcgcgcctgg ccgaggaact ggcgctcccg
     2581 cttatcttct accatcaacc cggcatgacc gggaatgcgt gcgacgtgga taccatcgat
     2641 cagatcgtga acatgccgcg cgtggccgcg ctgaaggatt caagctgcaa cttcgggctg
     2701 atcgcggtat tgctgcggcg ttaccccgac agggcgacgc gcccgttcag cctgctccag
     2761 ggagacgagt cgctattcga catcacgctg ctcctaggcg ccgacggcgt gatttccggc
     2821 ggtgggaccg ccttcatccc gttgctgctc gagctgtacc gcgccgcgtc ggccggcgac
     2881 cgcgagcgcg cgtacgctct caacgcccaa ttccgtgcgg cgctgctcga aatgctgggg
     2941 ccgaatctga aagtggattg gatggccgcc gtcaaggaca cgctcaagca acgcgatctc
     3001 tgcgagaaca tctgcaccag tccctttgtt aatcgcttcc gcgtgctcga cgggcacggc
     3061 caaaccgcac tgctccgacc cgcggccgcc ggccggccgg gctgaggtta ttgccatgca
     3121 tctcgccgat ttgctcagcc tcaagggaaa agtctgtctg gtgaccgggg gcgcgggact
     3181 ctacggccga cacatcacgg aagccctcgc cgaagccggc ggcacagtcg tcaccgcttc
     3241 gcgctcgctc gaggccggcc agcatctggc ggacgaactg gcgcgccggg gactcgccgt
     3301 cgcggcgctg accgtcgatc aggccgatca cgaatcggtc atgcaactga aagcggaatt
     3361 ggagcagcgc cacggtcgcc tcgacgtgtt tattaacaat gccgtcgctc gcccgatgaa
     3421 gcgctacgag gacccgctgg cagcctgggc cgaatcaatg cgcgtcaatg ccaccggcat
     3481 gttcgacatc acccgcgaaa tggccgacct gatcgcccgc ggcggcggcg gctcgattgt
     3541 caacatcgcc tcgatgcagg gcgtctacgc ccccgacttc atgctctacg agggaaccgc
     3601 gatggattcc ccgcccgatt accatttcca caagggcggc ctgatcacgc tgacgcgcta
     3661 cctcgcccgc aagctcgcca cgcgcaacat ccgcgtcaat gccgtcagcc cgggcggctt
     3721 gctcggcgat caggaccccc aattcttggg gcgttacaac cagaaggtgc cgctcggccg
     3781 catggcgctg cctgacgaca tcaaaggcgt cgttgtcttt ctggcttcgc ccgcctccgc
     3841 ctacatcacg ggcgaaaata tcctgatgga tggcggcctg catgcctgag ccatccgctg
     3901 atgccgaccg gaggatgtga gcgtgggaag tttagcctgg cgctttgatc aacgcgaacg
     3961 tatttacatt aacgaagtgc tcgactccgg gtttgccagt tccaccaccg gcacgatgaa
     4021 tcagcgcctc gaggcggcct ttgccgcccg ctttggccga aaatacgcgg tcacctgcaa
     4081 ctccggcacc accaccctcc atgccgcgct ggtcgccatc ggcgtcggct tcggcgatga
     4141 ggtcatcacc acgccgttga ccgtcatctc ctgcatgaac gccatcgttt attgcaatgc
     4201 cattccggtc ttcgccgatg tcgatcccaa tacctttctg ctcgatccgg atgatgtcga
     4261 gcgcaaaatc actccgcgga cccgggcgat catgcccgtt catctctacg gccaggtttg
     4321 cgacatgacc cgcatcatgg aaatcgccca gcgccacgat ctggccgtcc tagaggattg
     4381 cgctcaatgc tacctcggca cgcaccgcgg ccgcatcggc ggaacgttcg gcaatgccgg
     4441 aagctggagc tttgaaaact cgaagcacct gactaccggc gacggcggca tcctcgcctg
     4501 cgacagcgag gaactgggca cgcgactgcg caagctctgc tgccagggct tccgcacctg
     4561 tacggccgtc agcggccagg tgcgcctctc gaaggatgat tttcagaacc ccaactacaa
     4621 gcggcacgat tcgttcggct tcatgtaccg cctgcccgaa gtcgccgccg cgatgggcct
     4681 cgcccagctc gaaaagatcg actggttcgt cgccaagcgc gaacagatgg cccgtctgta
     4741 cgccgaggcc atccagggcg tcgattggct ggttccgcag cagacaccgg ccggcgaccg
     4801 caattcctat tacactttcg ccctgcgctt cctgaaaaag gacgtctcct gggaagcctt
     4861 ccgcaaaaaa cacattgaat acggcggcga cggcgcctat gccgcatgga ccctctgtta
     4921 tctcgaggat tcggttcccg aaatcctggc cctgttccgc cgaatggggc ttgaccggcg
     4981 catggcgacc gaccgcggca tctgccccca tgcagagatg atccagcgcg aattgatgca
     5041 gttcaccacc aatcagaagg atgaggatga gatgaaggtt caggccgaag cgctttacag
     5101 gaccgtgcgc tatttttcct gagccggtgg gagctcgcgt gcgctgcatt gcttcggttc
     5161 cattcggcct cgtctggcct caggccgcgg caacgctttc tggctcgttt gggctgcgat
     5221 gaacaaggat aattggaatt aatggataga tgagccgcga gatattttgc cgatgagacc
     5281 tgcacaatga aatggcggac gcgcgctggc catctcgtcc tgctgattgc aatgtttgca
     5341 tgggattccg ccgcccgggc ggccgacggg tccgactggg tcctgcccca tcggtttcgc
     5401 gtcgcgatcg actgcgacac cggcgaccct gcctccacca attccctcgc gtgcctggag
     5461 ctcgatctga atagattgtt gtccgacaac tcgatctcag gcgttgtcag ctattcatcg
     5521 cttcgattga tgcgtattgg cgctcaccag gagttgtcca ccaataccat ccaaacggaa
     5581 cttccgttcg cgctgacggg agacacgcgt tcctacgggc cggtcacgtt gtggtggcgc
     5641 gctccggatg cgtcagcccg aagttattat ctttattttg aaacggaatt ccgcgcggcg
     5701 gtcgcaggcg aggccgctcc ttttccgact tcaggaccga tcggcatcgg cgataccttc
     5761 cactaccatg acggacagcc cggtccggcc gactgcgtgc ccctgcactc gaccttctgg
     5821 ccgctcgact gggatggcga cggcgtcttc gacctcatcg gcatcgcctt ccgcatctat
     5881 gaacacggcg aggaccttga agagaatctg ggcaatggcg tttattttct gaaaaatatc
     5941 ggctccaatg aaaatcctct gtttgcgccc cggcttcgca tgaaggatcg taatggcgca
     6001 tggctgcgca ccgaccaact cggccagaac atgcatccga tggactggga cggcgacggc
     6061 gacatggatt ttgccggcgt cgccggaaac acgctcctgc tgtgggaaaa caccggctcg
     6121 cgcgacgccc acggcctgct gatgctcaat caacccgtcc cgatcatgga tcttcctgtc
     6181 tcgcagttcc gcgccgaaat gcccggcgtc gttcggacgc cgagcttcac ctgcaagggt
     6241 ttgcgacggg ttgactggga gggcgacggc gatctggacc tgatcgtcgc gtgggcctgc
     6301 gtcaatctgt tgaaggaggt cgacccgacc acgggcgtca tgccctacgg cgccgccctg
     6361 atgcatttcg aattgtatga acgcgtcggc gacgaccagg g
//
LOCUS       DAOPCO010000232         6351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10786_length_6351_cov_5.017471, whole genome shotgun sequence.
ACCESSION   DAOPCO010000232 DAOPCO010000000
VERSION     DAOPCO010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10786_length_6351_cov_5.017471"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2129
                     /locus_tag="PLS90_13570"
     CDS             <1..2129
                     /locus_tag="PLS90_13570"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPK03472.1"
                     /translation="TERKTPEELARAEAARLRFESGRGTKPPTPGGGPPGLSFVWGMI
                     FGGLTLLVIVLGYFAFRPERRDPGGQVAAPEAMPAVDSAAAVEAAGGTAPPAAASSTP
                     TPSPSPTPTPLAEATATPATPADVEPIRQALAASDWNRARTLAEAALKTQPGDPQIEP
                     LLYEAVYQQQTALARGALEQGQWITAQNALGEAMAVRPDDPALEGMLRRLPRLVLPSV
                     EQDVFALALAGGERVAAALKYEQLAVYGLKPPHRIQQVEGGRNYQAAFSPDLSRAAAA
                     ARVSETTSELALYDLQTGRRTPLTGAVATTVESLEYSPAGNWIAAAATWPRRQLTEWG
                     FGDELAQAEREGRLRASATELPAVVLWGTEDARPARLLLPGGEGERIDSIAFSPDEKR
                     VASSLRGGQIMVQEVDDGRIIQFLPAPFERRWLSYAPRGNRLFVTDTSDAVVIDLDEA
                     RTMARIESEPAGIRAWAFTPDGELLAVAGEGAPIRFHDVETGRELARLGEAQGRFHDL
                     DFSPDGRWLAAGGAQPGARLVVYPVDPAAIRQAAPAVAVPAAATPNATPTPAATDRLI
                     VRPETDAERPRVDLEEDVAYPAEAEEDERAAAEAAAEDEIRVGNVAAGGALTDLGLVY
                     ATRVLEAVNDRFQPPRSRRGARAVIEVIIAPDGRLEKPQLIRSAGRGELDRAALRAIA
                     RARAPRPPEDSRGRETLRTVRLTFNF"
     gene            complement(2135..3511)
                     /locus_tag="PLS90_13575"
     CDS             complement(2135..3511)
                     /locus_tag="PLS90_13575"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HPK03473.1"
                     /translation="MPMTMTEKILARAAGRDHVEAGDNVWINVDTLMTHDVCGPGTIG
                     IFKERFGANARVWDPDRVVIIPDHYIFTANPQAHRNIEILRDFVREQGIRYFYDPDFV
                     KDEGMPSPYADPARTHYKGVCHTALAEEGHNRPGEVLFGTDSHTCTSGAFNMFASGVG
                     NTDAAFILGTGKTWVKVPPTMRFVFEGELPEWLMAKDLILAVIGEIGVDGATYRAMQF
                     DGEGVLSLPIEERMTLTNMAIEAGGKNGIIPCDAETERYVRERTRRAGTRAEFSPVAA
                     DPGARYAYEKVFRIGQIEPMVAKPHSPDNKAFVSEVAGTKITRAYIGSCTGGKTGDFL
                     AAARLLKGNEVRVPTFIVPSTTEVDASLDQLTVDGVTLRQIFADAGCRIGPASCMACL
                     GGPADTFGRLNSADEVCISATNRNFPGRMGHKQSQVYLASPLTVAASALRGVITDPRD
                     VMAGVAAV"
     gene            4273..>6351
                     /locus_tag="PLS90_13580"
     CDS             4273..>6351
                     /locus_tag="PLS90_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03474.1"
                     /translation="MESRPGAAPALSQIARIGLTLLLTGLLLLLAAAGTACSGRSKAQ
                     KLHDQAVLLFKHGSYYKAIEKLEEALQVQPERADSIHLLADAYLKTGKPELAMQHARK
                     ALEIDPDADQAYLVLGQAYLAQALEKAEPDETGNARLSQTELDEAARIADELRQRKPD
                     SVEGLLLQARIDFLNDRLDSAARLYEKALELRADDATGRLGLIEVQMAREEYAEAEKQ
                     ARALVEGQEIPDLRALAFLARALANQNRHGEAYALLQPHVGADNKEPDLNLYLTAGEV
                     LLMQFDALRTTEEGEAAGTELGPAQGNEAAWEEAVQRLADLGTAMKGIYPNRPESFLF
                     RGISYQLQGEMDLAVEQMGRAVEMAPGSKRYRMALSAAQMLAGEHEAARREMRTLLKF
                     APGDLDARIRIAQTYLAEADFEQAREMLEPLAEEAPQNARINAMLAEAMLLSGDRQTV
                     EQALNAIDEAGVEQSIKPGAREFLLAQTAMREGVDLQGQGRIEQGAERFREAEELFAA
                     SLEIDPANPMAHLRLGELAERRGDILGALEHARAAAELNTAYRPYEARLYMQLGQYDA
                     ADRIYESMQDDAEDPVTWLLARAEVAERKGDTSSALGQYDKILREHPDDSRAYLQKAT
                     LLVNQSKPEEAARVLREGLERFPDQVGMRLGMARVYVQQDELDQAAAQLRELIDLINK
                     RIAAEKAQGRS"
BASE COUNT         1033 a         1834 c         2544 g          940 t
ORIGIN      
        1 ccaccgagcg caagacaccg gaggaattgg cccgggcgga agcggcgcgt ctgcgatttg
       61 aaagcgggcg ggggacgaag ccgccgacgc cgggaggcgg gccgccgggc ctgtcgttcg
      121 tgtgggggat gatattcggc gggctgaccc tgctggtcat cgttctgggt tactttgcgt
      181 tccgccccga gcggagggat cccggcgggc aggtcgccgc gccggaggcg atgccggccg
      241 tggattccgc tgccgcggtc gaggcggccg ggggaacggc gccgcccgcc gcggcgtcgt
      301 cgacgccgac cccgtcgccc tcccctaccc cgacacccct ggccgaggcg accgccaccc
      361 cggcgacgcc ggcggacgtg gaaccgatcc ggcaggcgct ggcggcaagc gactggaatc
      421 gcgcgcgcac gctggccgag gcggcgctta agacgcaacc gggcgacccg cagattgaac
      481 cgttgctgta cgaggcggtc taccagcaac agaccgcgct ggcccgcggc gcgctggagc
      541 aaggccagtg gatcacggcg cagaacgcgc tgggcgaggc gatggccgtg cgaccggacg
      601 atccggcgct ggaggggatg ctgcggcggc tgccgcggct ggtgttgccg tcggtcgagc
      661 aggacgtgtt tgcgctcgcg ctggccggcg gcgagcgggt ggcggccgcg ctcaagtatg
      721 agcagctcgc ggtgtatggg ctgaagccgc cgcatcggat tcaacaggtc gaggggggtc
      781 ggaattatca ggcggcgttc agtccggatc tgtcgcgggc ggccgcggcg gcgcgcgtga
      841 gcgaaacgac ttccgagctg gcgctctacg atctgcagac gggccgccgg acgccgttga
      901 ccggcgcggt ggcaaccacg gtggagagcc tggaatacag tccggcgggc aattggatcg
      961 cggcggcggc gacgtggccg cgccggcaac tgaccgagtg gggcttcggc gacgagctgg
     1021 cgcaggccga acgggaaggc cggctgcggg cgtcggcgac cgagctgccg gctgtcgtgc
     1081 tgtgggggac ggaggatgcc agaccggcgc gcctgctcct gccggggggt gagggcgagc
     1141 gaatcgacag catcgccttt tcaccggatg agaagcgggt ggcgtcgtcg ctccggggcg
     1201 ggcagatcat ggtgcaggaa gttgacgacg ggagaatcat ccagtttttg ccggcgccgt
     1261 tcgaacggcg gtggctgagc tatgcgccgc gcggcaaccg gttgttcgtc accgacacgt
     1321 ctgatgcggt ggtgatcgac ctggatgaag cgcggacgat ggcacgcatc gaatcggagc
     1381 cggcggggat ccgcgcgtgg gcgttcaccc ccgacgggga gctgctggcg gttgcggggg
     1441 agggggcgcc gatccggttt cacgacgtgg aaaccggacg cgaactggcg cggctgggcg
     1501 aggcgcaggg acggttccat gatctggatt tcagcccgga cgggcggtgg ctggcggcgg
     1561 gcggcgcgca gcccggcgcc cggctggtgg tctatccggt ggatccggcg gcgatccggc
     1621 aagccgctcc ggcggtggcg gtcccggcgg cggctacacc gaacgccacg ccgacgcccg
     1681 cggcgaccga ccggctgatt gtgcggccgg aaaccgacgc ggaacgcccc cgcgtggatc
     1741 tggaggagga cgtggcgtat ccggccgaag cggaggagga cgagcgggcg gcggcggaag
     1801 ccgcggcgga ggacgagatc cgggtgggca acgtggcggc ggggggcgcg ctgaccgatc
     1861 tggggctggt ctacgcgacg cgggtgctgg aagcggtcaa cgatcggttt cagccgccac
     1921 gctcgcggcg cggggcgcgg gccgtgattg aggtgatcat cgcgccggac gggcggctgg
     1981 aaaaacccca gttgatccga tcggcggggc ggggggaact ggaccgtgcg gcgctgcggg
     2041 cgatcgcgcg ggcgcgggcc ccgcgaccgc cggaggacag ccgggggcgc gagacgctac
     2101 ggacggtgcg cctgacgttc aacttctgag aagactagac ggcggccacg cccgccatca
     2161 cgtcgcgcgg gtcggtgatg acgccgcgca gggcgctggc ggccaccgtc agcgggctgg
     2221 ccaggtagac ctggctctgc ttgtgcccca tgcggccggg gaagttgcgg ttggtcgccg
     2281 agatgcagac ttcgtcggcg ctgttgagcc ggccgaaggt gtcggccggt ccgcccagac
     2341 aggccatgca ggaagccgga ccgatgcggc agccggcatc ggcgaagatc tgccgcagcg
     2401 tgacgccgtc gacggtcaac tgatcgaggc tggcatcgac ctcggtcgtg gaggggacga
     2461 tgaaggtcgg cacgcggacc tcgttgccct tgagcaggcg ggcggcggcg aggaagtcgc
     2521 cggtcttgcc gccggtgcat gagccgatat aggcgcgggt gattttcgtg ccggcgacct
     2581 cgctcacgaa ggccttattg tcgggcgaat ggggcttggc gaccatcggc tcgatctggc
     2641 cgatgcggaa gaccttttcg tatgcgtagc gcgcgcccgg atcggcggcg acgggggaga
     2701 attcggcgcg cgtgccggcg cgccgggtcc gctcgcgcac atagcgctcg gtctcggcgt
     2761 cgcaggggat gatgccgttc ttgccgccgg cctcgatggc catgttggtc agggtcatgc
     2821 gttcctcgat cgggagggag aggacgccct cgccgtcgaa ctgcatggcg cggtaggtgg
     2881 cgccgtcgac gccgatctcg ccgatgacgg ccaggatcag gtccttggcc atcagccact
     2941 cgggcagttc gccctcgaag acgaagcgca ttgtgggggg caccttgacc caggtcttgc
     3001 cggtgccgag gatgaacgcc gcatcggtgt tgccgacgcc ggaggcgaac atgttgaagg
     3061 cgccggaggt gcaggtgtgg ctgtcggtgc cgaagaggac ctcgccgggg cggttgtggc
     3121 cttcctcggc cagggcggtg tggcagaccc ccttgtagtg ggtgcgggcg gggtcggcgt
     3181 agggcgaggg catcccctcg tccttgacga aatcggggtc gtagaagtag cggatgccct
     3241 gctcgcggac gaagtcgcgc aggatttcga tgttgcggtg ggcctgggga ttggcggtga
     3301 agatgtaatg gtcggggata atgacgacgc ggtcggggtc ccagacgcgg gcgttggcgc
     3361 cgaagcgttc cttgaagatg ccgatcgtgc cggggccgca gacgtcgtgg gtcatgaggg
     3421 tgtcgacgtt gatccagacg ttgtcgccgg cctcgacgtg gtcgcgcccg gcagcgcgcg
     3481 ccaggatttt ctcggtcata gtcatcggca tgtgcgggat cctctcgtcg cggggagggt
     3541 ggagtggctc ccctgaaccg cgggtcggcc cacggtgctt gcaattgttc atggtagagg
     3601 aaggggagag ccagatcaag caggtccggt aatgcatcca actgtgcacc accaagtggt
     3661 gttcccaagg cgcgcgggaa ggagggcctg ataccgaagg gggaaggatg acatcgactt
     3721 ggcggcgttg gggaggaaat cgttgaaacg gttgggaaca acatattgcg ggcggtcacc
     3781 gggccgaggc ccggtgccaa tgcgaggccc ccccgagggg gcgcaagaac gcccgctgag
     3841 gcgggctctc caaggcgcca gtacaagtgg aatgcgagat atcccactga gggagactga
     3901 gacgcccgcg ttgacactca gcctgtcatg ggaagttcgc ttccgccttc ttaagtcccc
     3961 ttggtccctg atctccctac tggctcccca agggaggaac cttgccagct gagaatggtt
     4021 ttcgatggcg ggcggggggt ggagtgccta caatcggccg tcaagagcat gcctggccga
     4081 atggcgacgg aaggtgaagc ggcccggtcg aagtcgcctg cggtgagccg atccctgacg
     4141 ggcggccggc ctccctccga tgggccaacc ctcaaaatct tcaagttacg ttgcctgaaa
     4201 gccgaaatgc taaggagacg cacggactgt ccgtctggtc ggagagtcgt ggattcgcag
     4261 gaggcgactt gtatggaatc gcgcccgggc gccgcacccg ccctgtccca gatagcccga
     4321 attggcctga cgttgctgct gaccggtctg ctgctgctgc tggcggcggc cggcacggcg
     4381 tgctcgggcc ggagcaaagc ccagaagctg cacgatcagg cggtgctgct gttcaagcac
     4441 ggcagttact acaaggcgat cgagaagctg gaggaagcgc tgcaggttca gcccgagcgg
     4501 gccgacagca tccacctgct ggccgatgcc tacctgaaga cgggcaagcc ggaactggcg
     4561 atgcagcatg cgcgcaaggc gctcgagatc gatcccgacg cggaccaggc gtatctggtg
     4621 ctgggccagg cctacctggc gcaggcgctg gagaaggccg agccggacga gacgggcaat
     4681 gcgcggttgt cgcagacgga gctggacgag gcggcgcgga tcgcggacga gctgcgccag
     4741 cgcaagccgg attcggtcga ggggctgctg ttgcaggcgc ggattgattt tttgaacgac
     4801 cggctggatt cggccgcgcg gctgtatgag aaggcgctgg aactgcgggc ggacgacgcg
     4861 acgggccggc tggggctgat cgaggtgcag atggcgcgcg aggaatacgc cgaggccgag
     4921 aagcaggcgc gcgcgctggt ggaggggcag gagatccccg acctgcgggc gctggcgttt
     4981 ctggcgcggg cgctggccaa ccagaaccgc catggcgagg cgtatgcgct gctgcagccg
     5041 cacgtggggg cggacaacaa ggaaccggac ctgaacctgt acctgacggc gggcgaagtg
     5101 ctgctgatgc agtttgacgc gctgcggacg acggaggagg gggaggcggc cggaacggag
     5161 ctgggaccgg cccaagggaa cgaggcggcg tgggaggagg cggtgcagcg gctggccgac
     5221 ctggggacgg cgatgaaggg gatttacccg aaccggcccg aatcgtttct cttccgcggc
     5281 atttcatatc agcttcaggg cgagatggac ctggcggtgg agcagatggg ccgggcggtg
     5341 gagatggccc ccggcagcaa gcggtaccgg atggcgctgt cggcggcgca gatgctggcg
     5401 ggggagcatg aggcggcgcg gcgtgagatg cgcacgctgc tgaagttcgc gccgggcgat
     5461 ctggatgcgc ggatccggat cgcgcagacg tatctggcgg aggcggattt cgagcaggcg
     5521 cgcgagatgc tggagccgct ggccgaggag gcgccgcaga atgcgcgcat caacgcgatg
     5581 ctggccgagg cgatgctgct gtcgggcgac cgccagacgg tggagcaggc cttgaacgcc
     5641 atcgatgaag cgggggtgga gcagagcatc aagccgggcg cgcgggagtt tctgctggcg
     5701 cagacggcca tgcgcgaggg ggtggatttg caggggcaag ggcggatcga acagggggcg
     5761 gagcggttcc gcgaggccga agagctgttt gccgcctcgt tggaaatcga tccggcgaac
     5821 ccgatggcgc atctgcggct gggcgaactg gccgagcggc ggggcgacat cctgggggcg
     5881 ctggaacatg cgcgggcggc ggcggaactg aacacggcct accggccgta tgaagcccgg
     5941 ctgtatatgc aactggggca gtatgacgcg gccgaccgga tctatgaatc gatgcaggac
     6001 gacgccgagg acccggtgac gtggctgctg gcgcgggccg aggtggcgga acgcaagggg
     6061 gatacgtcga gcgcgctggg ccagtatgac aagatcctgc gggagcatcc ggacgattcg
     6121 cgggcgtatc tgcagaaggc gacgctgctg gtgaaccagt cgaagccgga ggaggcggcg
     6181 cgcgttctgc gcgaggggct ggagcggttt ccggatcagg tcgggatgcg gctggggatg
     6241 gcgcgggtct acgtgcagca ggatgaactg gaccaggcgg cggcacagtt gcgggagctg
     6301 atcgacctga tcaacaagcg aattgccgcg gagaaggcgc aggggcgttc g
//
LOCUS       DAOPCO010000233         6335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10824_length_6335_cov_3.447134, whole genome shotgun sequence.
ACCESSION   DAOPCO010000233 DAOPCO010000000
VERSION     DAOPCO010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10824_length_6335_cov_3.447134"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLS90_13585"
     CDS             <1..547
                     /locus_tag="PLS90_13585"
                     /inference="COORDINATES: protein motif:HMM:NF027452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HPK03475.1"
                     /translation="MSSDDYYVPLTQPSRVNDEIHDALLGWLDQTSNLYLYDYYGNRS
                     EFFPIIDTLRKDYAYYQTIGLDKLSSECFLWPEFNMWAYGRLAWNHQIPLRQLVEDFC
                     RIAYRDAQQPMSEFYLALEGYKWEWPQHQAELAALLKQAQTKAGDDPTVQAKLERLHV
                     LLATTPNKSWEHPQPPPPLSE"
     gene            710..2548
                     /locus_tag="PLS90_13590"
     CDS             710..2548
                     /locus_tag="PLS90_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03476.1"
                     /translation="MMFRSMVLLALGWATLGMESGPAADERFWLVRDGVPTADIYAST
                     AEWDTAEWLVKRIQQWTGQQLLLLDADAPLPASRASMGRLLLGTTEGSDEITRVLGAD
                     SSFQNLNDEGYALLATTDPPRLIVAGRSSIATRYAAAELLNYRLEVTPTDVWCEPFEV
                     FEQPALPSRWFWISTSYAHWDDSYGGPHITDEMTRTHGPLPDGTDLHQVAYPGQMMGE
                     EPYVDTYKAMIDWMSEHKLNGAMIFGYLSNGLEPAREIAAYGQKRGVGIIAGVGTMSY
                     WGAYYGGNNIMNLDTLVKIHPEWQVEDERGIGYLCPSIPEVQAYWRRAGGWLAEALPE
                     LDGLYLENGDFMMCPCDVCAAARALPENDTRFYWDMMASSVPFIEGSSASQPDWNYVY
                     ATYTGFGPEALAKGTDHVPPRFPEQFPTDAICQWTVTGLNESNWPEDLMAPTQRSVGL
                     FHSPSVWGTPDGEDRWWAGPGSSHDDASRLVRFYCNRMAETGFEGLIVKGMKNHHSPG
                     PMLTYLALGEFSWHPDRTIEEWETERLGRLFGGRENAATYLRLARDSSRDPEARREMI
                     VEAVGIANSPELSPRARPYWQDLVTELEYRARLNDLLLSEQEKEKD"
     gene            2559..4997
                     /locus_tag="PLS90_13595"
     CDS             2559..4997
                     /locus_tag="PLS90_13595"
                     /inference="COORDINATES: protein motif:HMM:NF015604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046559 - alpha-glucuronidase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucuronidase family glycosyl hydrolase"
                     /protein_id="HPK03477.1"
                     /translation="MRPNTILAWGMLGILFSGSPGWAAHFSLVTDGEAAAVIYAPGEE
                     ASAGLRLRDRVHEWTGAALAVHSQGRPHTDSPLIAIGTVQTNEAVRAALEGDARLQTL
                     GEQGFILKVVRSGETQILLAAGNTPQGVNNAISELVSWELRLDDRNAWVAAELDKAER
                     PGLKYRLLWGQATDPWAPTLEETIQITNGRAPNPAQDSAEKFLNFNQRRIDYASDHKL
                     NGLILYYLINEKHGGLAAAQDLTRWGRLNNVRILSGVATMSSYHGFTMNENHRFHFKE
                     WNRLHPELRLVGRDGEFMEGICPCKPLNQAWLREGTRWFLETLPDLGGVNLENGDWII
                     CWSEDCLAARHQPENDPNFFWDQMATYQPCLDAADAVRPDAWLTFATYAGFSEGAIQG
                     AMHGAIQTRRAAGDSTRIVYPPKFVKQLPHNAVCQWTLTGMTGPEGWPEGLRPPESTF
                     KEHIGLLHQSSPWWGPTDPEKWWADEGPGSTWEDITELIQFMCSRLDQAGMHGLVLKG
                     LTAASAPAKELNYLALEYFGWYPQNTYEQFINERLSVALGGRERAELFLRLLRNTTRD
                     PDAIERDRQQALRSAQARDLDVRQRERWKNLATELRRRSELARDMESEAGRVKDQMVI
                     KPNPQQEGFILVTSGEIKTGGDMIEWGWQGGNAIIPFGMHTVFGLTAPDVKEARLRIP
                     LTDIPRSPDTSATTQRWVVQHFIPADPSAISEADVSSSSLGNIGVYREPGEPKLSGDA
                     FREFDITALLKQALQGGRTTFAWRILPEGDAPAADSRLAFPAGASTNISFPPENRGAR
                     LIITRYTLVGSEAP"
     gene            5225..>6335
                     /locus_tag="PLS90_13600"
     CDS             5225..>6335
                     /locus_tag="PLS90_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03478.1"
                     /translation="MFTARRAIVWIALAWAFTGVRATAEALPPAVFENVQDYTYMWWA
                     HGLRDDRKIFNIQTSNYALSFDWDQFALKSLTPLVDPPDEAEALTMSNEAVFDQAHRT
                     QFSDSFDTGIANSPLHGQVADNMLGGTQESTWTAPDGVMYQPHPTQSNMIIVGSYNVA
                     IANHAVDPALTYRMKMKAAVSDGPLESGSADGKFACVSILKDPDKLDFWDTSSGLTVR
                     LFNNSTGNTRLDMFYGRGTTTGNPATDYGGWVGGSLDTGDLDEEGYWPVMLEWRGAGS
                     PSHPLDLKVYVNGHLRAHYENITDLIGGDRVGFAAPYGGCFDDFELIRCVQSPRLECA
                     VEVGEARYKAISIGANYASSKIVDTGKFFQRRWMEN"
BASE COUNT         1334 a         1794 c         1926 g         1281 t
ORIGIN      
        1 catgtcttct gatgattatt atgtgcccct cacacagccg agccgggtga atgacgagat
       61 tcatgacgcc ttgttaggct ggctggatca gacatcgaac ctgtacctat acgattacta
      121 cggaaaccga agcgagttct ttccaatcat cgacacgttg cgcaaggatt acgcctacta
      181 ccaaacaata ggattggaca agctttcctc tgagtgcttc ctttggcccg aattcaatat
      241 gtgggcttat ggccgcttgg catggaatca tcagattccg ctacgccagt tagtcgagga
      301 tttctgtcga atcgcctatc gcgatgcgca gcagccaatg agcgagtttt acctggcact
      361 cgaaggctat aaatgggagt ggccgcagca tcaggcagaa ctggcggcgc tgctgaaaca
      421 ggcgcaaact aaagcagggg acgatccgac cgttcaggcc aaactggaac gtctccacgt
      481 cctgctggcc accacgccga acaagtcttg ggagcacccg cagccgcctc cgccactgag
      541 tgaataatgg aatcggcaat gggatgcgtg aaggccaatc gggactctga gcctgcaccg
      601 tcggcgttgg tatacagttg tgtcgacatg gccttgaaaa tggcatctcc agtgggtggg
      661 acatgaatct ccttccgtaa gaagctgatt gttctaaaag gagcggctca tgatgtttcg
      721 ttcgatggtc ttacttgcgc tgggatgggc gacgctgggg atggaatcgg ggcctgccgc
      781 cgacgaacgt ttctggctgg tccgcgatgg cgttcctacg gccgatatct atgcctcgac
      841 cgctgaatgg gacacggcgg aatggctcgt caaacggatt cagcaatgga cggggcagca
      901 attactgctg ctcgatgccg atgctcccct ccccgccagt cgggcatcca tgggacggct
      961 gctgcttggc acgaccgaag gcagtgacga gatcacgcgc gtcctgggtg ccgacagctc
     1021 attccagaac ctcaacgacg agggctatgc cctgctcgca accaccgatc ctccccggct
     1081 gatcgtcgcc ggccgcagtt caattgccac gcgctacgcg gccgccgaac tgctcaacta
     1141 ccggctcgag gttacgccca ccgatgtctg gtgcgaaccc ttcgaggtgt tcgagcaacc
     1201 ggcgcttccg agccgctggt tctggatcag caccagctat gcacactggg atgattccta
     1261 tggcggccct cacatcaccg atgaaatgac acgtacacat ggtcctctgc ccgatggcac
     1321 tgatctgcat caggtggcct atccaggtca gatgatgggg gaagagccct atgtagacac
     1381 ctacaaggcc atgatcgact ggatgagtga gcacaagctc aacggcgcca tgattttcgg
     1441 ctatctttcg aacggcttgg agccggcgcg tgaaattgcc gcctatgggc aaaagcgcgg
     1501 cgtcgggatc atcgcagggg tcggcaccat gagctactgg ggcgcttatt acggcggcaa
     1561 caacatcatg aatctggata ccctggtcaa aatccatccc gagtggcagg tcgaagacga
     1621 gcgcgggatc ggatacctgt gcccatcgat tccagaggtc caggcctatt ggcggcgggc
     1681 cgggggctgg ctggcggagg cgctgcccga gcttgacggc ctttacctgg aaaatggcga
     1741 cttcatgatg tgcccctgtg atgtgtgcgc agcggctcgc gctcttccgg agaacgatac
     1801 gcgattctat tgggacatga tggccagttc cgtgccgttt attgaaggat catcggcctc
     1861 ccagcccgat tggaactatg tatatgccac ctataccggg tttggtcccg aagccctcgc
     1921 caagggaact gaccatgtgc cgccgcgctt ccctgagcag tttccgaccg atgcaatctg
     1981 ccagtggacc gtgaccggtt tgaacgaaag caactggccg gaggacctca tggcgccgac
     2041 gcaacgtagt gtcggcctgt tccacagtcc gagtgtctgg ggtacgcccg atggagagga
     2101 tcgttggtgg gccggccccg gcagttcgca tgatgacgcg tctcgcctgg tccgctttta
     2161 ctgtaaccgg atggcggaga ccggatttga gggattgatt gttaaaggga tgaaaaacca
     2221 ccacagtccg gggccgatgc tgacctacct ggcgctgggg gaattctcct ggcatcctga
     2281 tcgcacaatc gaggagtggg aaaccgaacg tctcgggcgc ctgttcggcg gtcgcgaaaa
     2341 cgccgcaacc tacctgcggc tggcgcggga tagttcgcgc gatcccgagg cccggcgcga
     2401 gatgatcgtc gaggccgtcg gcattgcgaa ctcgccggaa ttgtctccgc gtgcccggcc
     2461 ttattggcag gatctggtca ctgagctgga gtaccgtgca cgcctgaacg atctgctgtt
     2521 aagcgagcag gagaaagaga aagactagag tggtgaaaat gcgcccgaac acgatcctcg
     2581 catggggaat gctcggcatc ctgttcagcg gatcgccggg ctgggccgct catttctcgc
     2641 tggtcacgga tggggaggcc gccgccgtga tatatgctcc cggcgaagag gcatcggccg
     2701 gcctgcgact gcgtgatcgg gtgcatgaat ggaccggggc ggccttggcc gtgcacagtc
     2761 agggccgtcc gcacacagac tcgccgctga ttgccatcgg caccgttcaa acgaatgaag
     2821 cggtgcgtgc cgcgctggag ggcgatgctc gtctgcaaac actgggcgaa cagggtttca
     2881 tcctgaaggt ggttcggtcg ggtgagacgc aaatactctt ggcggcgggg aacacgcctc
     2941 agggggtgaa caacgccatc agtgaactgg tcagttggga acttcggctg gatgaccgga
     3001 acgcctgggt cgccgctgaa ctcgacaagg ccgaacggcc cggcctcaag tatcggctgt
     3061 tgtggggcca ggcaacggat ccgtgggcgc ccacgctcga agaaacaatc cagatcacga
     3121 atgggcgcgc cccaaacccg gcccaggact cggcggagaa gttcctgaac tttaatcagc
     3181 ggcgcatcga ttatgccagc gatcataaac tcaatggatt gatcctgtat taccttatca
     3241 acgaaaagca tggcggattg gcggcggctc aggacctgac acgatggggc cggctgaaca
     3301 acgtgcgcat tctctcgggc gtagcgacga tgagctccta tcacggcttc acaatgaatg
     3361 agaatcatcg ctttcacttc aaggaatgga accggttgca tccggagctg cgtctggtcg
     3421 ggcgcgacgg agaattcatg gaggggatct gtccgtgtaa acccctgaac caggcatggc
     3481 tgcgcgaggg gactcgctgg ttccttgaaa ccctgcccga tctgggcggc gtgaacctcg
     3541 aaaatgggga ctggatcatc tgctggtcgg aggattgcct tgccgcgcgt catcagcccg
     3601 aaaacgaccc gaatttcttc tgggatcaga tggctaccta tcagccgtgc ctcgacgcag
     3661 ccgatgcagt gcgccccgat gcctggttga cttttgccac ttatgccggt ttcagtgaag
     3721 gagcgatcca aggtgcgatg cacggcgcca tccagactcg acgcgccgcc ggtgacagta
     3781 ctcgcatcgt ctatcccccc aaattcgtca agcagctccc gcacaacgcc gtgtgccaat
     3841 ggacgctgac aggcatgacc gggcccgagg gttggcccga aggcctgcgc cccccggaaa
     3901 gcactttcaa ggagcatatt ggtctgctcc accagagcag cccctggtgg ggaccgaccg
     3961 atccggaaaa atggtgggcc gacgaagggc ccggttcgac gtgggaggat atcaccgaac
     4021 tgattcagtt catgtgcagc cggctcgatc aggccggcat gcatggactg gttctgaaag
     4081 ggctgaccgc tgccagcgcg cccgccaagg aacttaatta cctggcgctg gaatactttg
     4141 gctggtatcc gcaaaacaca tacgaacagt ttatcaatga gcggctgtcg gtcgctctgg
     4201 gcggccggga gcgcgccgag ctttttttgc ggctgctgcg taatactacg cgagatcccg
     4261 atgcaatcga gcgtgaccgg cagcaggccc tgcggagcgc gcaagcgcgg gatctggatg
     4321 tgcgacaacg tgagcgttgg aagaatctgg caactgagct gaggcgtcgc agtgaactcg
     4381 cccgcgacat ggaatccgag gcggggaggg tcaaggatca gatggtgatc aagcccaacc
     4441 cgcaacaaga gggatttatt ctggttacca gtggtgaaat taaaactgga ggcgacatga
     4501 tcgaatgggg ctggcaaggg ggcaacgcca tcattccatt cggcatgcac accgtcttcg
     4561 ggctgaccgc tcctgacgtc aaggaggcgc gtttgcgaat tcctctcacg gacataccgc
     4621 gttcgcccga cacctcggcc accacgcagc gctgggtggt tcagcatttt atccccgccg
     4681 atccctcggc catctccgaa gctgatgttt catcctccag tttagggaac atcggcgtct
     4741 atcgggaacc cggagagccc aagctctcgg gcgatgcgtt cagagaattc gatatcaccg
     4801 cgttgctcaa gcaagcgctt cagggtggcc gcacgacctt tgcatggcgc atactgcctg
     4861 agggcgacgc accggcggcc gatagccggc tggccttccc cgcgggcgca agcaccaata
     4921 tttcgttccc gcctgagaac cggggggcgc gcttaatcat cacgcgctac acattagtgg
     4981 gctcggaagc gccatgagga attgactaat ggaagaactc aggcacacct cacacgcttg
     5041 gagactgaaa ttcgcgtcag cggagattca ggccatgctt ggaatcgttg atttatcctg
     5101 atagagccgt ttatcgtaat gccattaatc gcattcattg acggttgggt gatgctatgt
     5161 taacaatgag tacctggatt ccagcgtcct gactggacag agacgtcata gaggggcaaa
     5221 ctgcatgttc actgctcgca gggccatcgt gtggatagca ctggcctggg ccttcaccgg
     5281 agtcagagcg acggccgaag cgttgcctcc cgccgttttc gaaaacgtcc aggactacac
     5341 ctacatgtgg tgggcgcatg gtctgcgcga tgaccgcaag attttcaata ttcagaccag
     5401 taattacgcc ctttccttcg attgggatca attcgcgctc aagagcctga caccgctggt
     5461 cgatccgccc gacgaagccg aggcgttgac catgagcaat gaggccgttt tcgatcaggc
     5521 ccatcgcacg caattcagtg attcgttcga taccggcatc gcgaattctc ccttgcatgg
     5581 acaggttgcc gacaacatgc tgggcggcac gcaggaatcg acatggaccg cgccggacgg
     5641 cgtcatgtat caaccccatc ccacccagtc gaacatgatt attgtcggga gttataatgt
     5701 cgccatcgca aatcatgcgg tggacccggc gctcacatat cggatgaaga tgaaggccgc
     5761 ggtaagcgac ggtcctttgg aatccggttc ggccgatggc aagtttgcct gcgtctcaat
     5821 cctgaaggat ccggacaagc ttgacttctg ggacacaagc tcggggctga cggttcgctt
     5881 gttcaataac tccaccggca acacacgcct ggacatgttc tatggacggg ggacaaccac
     5941 tggcaaccca gcgacggact atggcggctg ggtgggtgga tcgctcgaca caggcgatct
     6001 ggatgaggag ggttactggc cggtgatgct ggagtggcgc ggggcgggat ctccgtctca
     6061 tccgcttgac ttgaaggtgt atgttaatgg tcacctgcgc gctcattatg aaaacatcac
     6121 cgacctgatc ggtggcgacc gcgttggatt tgcggccccc tacggtgggt gtttcgatga
     6181 ttttgaactc atccgctgcg tccagtcgcc ccggctcgag tgcgccgtcg aggtcggcga
     6241 ggcccgctac aaagccatca gcatcggcgc gaattacgcc agcagcaaga ttgttgatac
     6301 cggcaagttt ttccagcgac gctggatgga aaata
//
LOCUS       DAOPCO010000234         6313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10863_length_6313_cov_3.948066, whole genome shotgun sequence.
ACCESSION   DAOPCO010000234 DAOPCO010000000
VERSION     DAOPCO010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10863_length_6313_cov_3.948066"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1593
                     /locus_tag="PLS90_13605"
     CDS             <1..1593
                     /locus_tag="PLS90_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03479.1"
                     /translation="EGLGEEGYILQTIRWKDRPTVVAAGRTTAGVNNAVSELITWRLE
                     LNGETATVPLGLDLAEKPALPYRILWSWDGEQNWPDTLAELDVREGPSPATDRDAFLT
                     HFQRAIDYASDHKLNGIIIWGFIRDERGGIETCRELIRYANRNNVRLLPGICTQSAYG
                     GFTYSKDSPFNLENWTTKHPELRFVHPRTGKPDIGICPSQPENIEWLREATRWFLETF
                     PDIGGVNLENGDWMYCICDSCKLARSKPGNDPNFFWDQMASYQPVMEEIQRLRPDIWL
                     TFATYVGYTEAELSGTIAQAKKTGFGEGGEVPPKMVFQLPAGGIYQWTFTNMAGAGGW
                     PDGARPPAYDRGDHIGLLHQGSIWGSPVDPARWWATPNANTKYDDISELLKAVCAKIQ
                     GAGLHGLVIKGQSGIVSPANELAYTAMEYFTWHPERSYEQFMEDRLSLLYGGDERAEL
                     FLELLRETDRDANVLKLHQERAAELAQSEEFESRQQVRWRNLADEIARRQRMLATAAE
                     SEAKGPANEEESFVAPPPPPLP"
     gene            1652..3541
                     /locus_tag="PLS90_13610"
     CDS             1652..3541
                     /locus_tag="PLS90_13610"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPK03480.1"
                     /translation="MRHIYMLIAATTLFHQGAWGTQDLTSLAEDRPTTGLQSELTSEI
                     DSIEALWAGFDPRSDPLEIEVIETIEEDGIRMERLYFTGETWQGEKVRVYAIIGAPTS
                     GENLPGILHIHGGGQTASLAWVRFWANRGYVCASHDYLGDWRAQDPNRNDFTIWHDVD
                     GNMAQGAGNNYNMEPDARHNSWYHWTLVARRVLTLIENHPQVDPERLGVFGVSMGGLQ
                     TWLVAGSDDRIKVAVPIYSCGWTSYRIPPDVEEESPTEVEANIEQWRRLLAPQSYASR
                     IQAPLLYMSASNDFHGLMDRTPQTLKRVGSKHVRQSFTPRYNHHIAPNHGQNLPLWMD
                     YWLKEQGDPWPASPALDLQGRQGAPVAIVTPDRPDEIDTVRIYYALNNPWPQSRFWRD
                     AVSLERDGNKWRAEIPFMNKGDRLYIFANVRYRSGVHLSSLLQTANAGDLTPSAPTVK
                     WSSMIDPMTDANDWFYFAAYTDPNIDQEYFIPWTDSTGEKGFTLNRELIGAGEARFQI
                     ATHKLGDPQWRGTGRKTLLLDVYNPHQPRELRIKAIERHWQPTRHEFVTEFDSPSEDP
                     SGWRTLRLDAAQFQDNEGQSMADWGAVDCLLIEGITSAEHSPVFRRLRWEMDSSEGKA
                     PAPSP"
     gene            complement(3568..4884)
                     /locus_tag="PLS90_13615"
     CDS             complement(3568..4884)
                     /locus_tag="PLS90_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03481.1"
                     /translation="MKSTNPDHFGSTRDSRRLLILSLIGGAILLCGWSWPEAEEKAPP
                     TDLFASRGSATCEFGAEFAAPGAPQEWLEHYGVIRYREPEPADRDFDLYFSPSLAIWK
                     FHRVDSSDQKAQYVGGFHYSECPGGQVTAMDRVIQPAIGLHPNYGTEIGMTNTMVRTN
                     YGCGTSFYSQPWPCDNYQGAGSGRGILPEIESPPAWDEATQTLRAAAVLVPWIFYEGI
                     LDPESNAFLNDFAVTYPMETALRIQQQIAAALDNVRMEIAIRIYPDGTIMLAHLFSDL
                     QGRPWIPYATDGHWIAFDSAPNGRVFYTSAGGQFVEPFAGGANPAGLSLYGWEMGGWA
                     GYFVDTVRPDIGIAFYIGHTTHGSSSISIFPSYNYPNAPAVMATNRWTLIEPFGKVFR
                     QHFLKVGTKEEMNEMAAVLNPHVQVCSIAPTPPPPETISRTWPAYH"
     gene            complement(4957..6012)
                     /locus_tag="PLS90_13620"
     CDS             complement(4957..6012)
                     /locus_tag="PLS90_13620"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK03482.1"
                     /translation="MKVTIRDLARLLNVSETTVSLAFQNNSPISDKTRQRVLEKAKEL
                     DYIPNLAARRLRRGHTKNKIIGFLVNDITHPFYSLMVRAAQTIAMSHGYTILVADSCW
                     QADKELAGIEYLLESRVDGILACLCERTPESLRLLERFLVPYILLDTVPSDYTKAFVA
                     NDLVEAARLAAKHLTQDVGCRYPVFLTADRDMEHFSAFDILGREFACSIREQGLPFDA
                     KQIIYAGLTIAAGQAAFGRVLETIPDVDGIFCINSLCALGVIEAADQLGIQVGRDVAV
                     MGVEDLDILHLSRISLTSIRQPFDQLAEMATTMLIHAIENKIHPSVQVSLKPSMIVRD
                     STKSKQKPALKSVARRK"
     gene            6175..>6313
                     /locus_tag="PLS90_13625"
     CDS             6175..>6313
                     /locus_tag="PLS90_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03483.1"
                     /translation="MFEALVIDRLCAAAVSAVGRRLGLSWSVSTRSCSRRAAPGVGAG
                     RE"
BASE COUNT         1369 a         1752 c         1899 g         1293 t
ORIGIN      
        1 gaggggctcg gcgaagaagg ctatattctg cagaccatac gctggaagga tcgtcccact
       61 gttgttgccg ccgggcgaac gacggccggc gtgaataacg ccgtcagcga attgatcaca
      121 tggcgcctgg agttgaacgg tgagaccgcc acggtgccgc tcggactcga cttggctgaa
      181 aagccggcgc tcccttaccg catcctctgg tcgtgggatg gcgagcagaa ttggccggat
      241 acgctcgccg agttggacgt ccgcgagggc cccagccccg cgaccgatcg cgatgcgttt
      301 ctgacgcact tccaacgggc gattgactat gcgagcgacc ataagttaaa cggcattatc
      361 atttggggct tcatccgcga cgagcgcggc ggcatcgaaa cgtgtcgcga gctgattcgt
      421 tatgccaatc gcaacaacgt gcgcctgttg cccgggatct gcacgcagag cgcctacggc
      481 ggattcacct atagtaagga tagcccgttc aatcttgaga actggactac gaagcatccg
      541 gaattacgat ttgtacatcc ccgcacgggc aagcccgata tcggcatttg cccctcccag
      601 cccgagaata tcgaatggct tcgcgaggcg actcgctggt tcctggaaac cttccccgac
      661 atcgggggag tgaaccttga aaatggcgac tggatgtatt gtatttgcga ctcctgcaaa
      721 ctcgctcggt ccaagccggg gaacgatccc aacttcttct gggaccagat ggcgtcctat
      781 cagcccgtaa tggaagagat ccagagattg cgtcccgaca tctggctgac gttcgccacc
      841 tatgtgggct acacggaagc tgagctgtcg ggcaccatcg cgcaggcgaa gaaaaccggc
      901 ttcggcgaag ggggcgaagt cccaccgaaa atggtttttc aattgcccgc gggcggcatt
      961 tatcaatgga cgttcaccaa tatggccggc gcaggcggct ggcccgatgg cgcgcgtccg
     1021 ccggcctacg accggggcga ccatatcggt ctgctgcacc agggaagcat ctggggcagt
     1081 ccggtggatc cggcgcgctg gtgggcgaca cccaatgcaa acaccaagta tgatgacatc
     1141 agcgaattgc tgaaagctgt gtgcgccaag attcaaggcg ccggtcttca cggattggtg
     1201 atcaaagggc aaagcgggat tgtcagccca gccaacgaac tggcctatac cgcgatggag
     1261 tatttcacgt ggcatcccga acgcagctat gaacaattca tggaggatcg cctcagcctg
     1321 ctctacgggg gggacgagcg cgctgaactc ttcctggaat tgttgcgtga aaccgatcgt
     1381 gacgcgaatg ttctgaagct tcatcaagaa cgcgcagccg aactggcaca gtcggaagaa
     1441 ttcgagtctc gtcagcaggt ccggtggcgt aatctggcgg atgaaatcgc gcggcgtcag
     1501 cggatgttgg cgaccgcagc ggaaagtgag gcgaaagggc ccgcgaatga agaggaatcc
     1561 ttcgtcgcgc cgccaccgcc gccgcttccc tgacgtcgag ttcgtcacca ccagatagcc
     1621 tgaattcgaa atcccccagg agcgaccgaa tatgcggcac atctacatgc ttattgctgc
     1681 cacgacgtta ttccatcaag gggcgtgggg gactcaggat ttaacctctc tcgcggaaga
     1741 tcgccccacg actggtttgc aatcggagtt gacctcggaa atcgacagca ttgaggccct
     1801 ttgggccgga tttgatccgc gaagtgatcc tcttgagatc gaagtgatcg agaccatcga
     1861 agaggacggc atccggatgg agcgcctcta tttcaccggc gaaacgtggc agggcgaaaa
     1921 agtccgtgtc tatgccatca tcggtgcgcc cacttcgggt gagaatcttc cgggcattct
     1981 acatattcac gggggtggtc agaccgcctc gctggcctgg gttcggtttt gggctaatcg
     2041 gggttatgtg tgtgccagcc atgattatct gggagactgg cgcgcgcagg accccaatcg
     2101 caatgatttt acaatatggc atgacgtgga tggaaacatg gcgcaggggg cgggcaacaa
     2161 ttacaacatg gagcccgacg cgcgtcataa tagttggtac cactggaccc tcgtggcccg
     2221 ccgagtattg acgttaatcg aaaaccatcc tcaggttgat ccggagcgat tgggggtttt
     2281 cggtgtgtcc atgggaggcc tgcagacatg gctggttgcc ggcagcgatg atcgtatcaa
     2341 agtcgcggtt ccgatctaca gttgcggctg gacaagctac aggattccgc ccgacgtgga
     2401 agaggaatcc ccgacggaag tggaagccaa tatcgagcag tggcggcgat tgctcgcgcc
     2461 ccagtcctac gcctcacgca ttcaggcgcc gctgctatac atgagcgcaa gcaacgattt
     2521 tcatggactg atggaccgga ctccccagac attgaagcga gtcggatcga agcatgtccg
     2581 ccagtcgttc acgccgcgat acaatcacca tattgcgccg aatcacggac agaatctgcc
     2641 gctatggatg gactactggc ttaaggaaca gggagatccg tggccggcat ctccggcgct
     2701 tgatctgcaa ggccggcagg gcgcgccggt ggcgatcgtg acacccgatc gtcctgatga
     2761 gattgacacg gtgcggattt attacgcact gaataatccc tggccccaga gtcgattctg
     2821 gcgcgatgcg gtttcgctcg agcgtgatgg gaataaatgg cgcgccgaaa tcccgttcat
     2881 gaacaaaggc gatcggctat acattttcgc aaacgttcgc tatcgctccg gcgttcatct
     2941 cagctcgttg ttgcagacag ccaacgccgg tgatttgaca ccctccgccc caacggtgaa
     3001 gtggtcgtcg atgatcgacc ccatgacgga tgccaacgac tggttttatt ttgccgcgta
     3061 tacggatccc aatatcgacc aggagtattt tatcccctgg acggatagca cgggggagaa
     3121 gggcttcacg ctcaaccgcg agttgattgg ggccggcgag gcgcgcttcc agatcgccac
     3181 gcataagctg ggcgatccac aatggcgcgg cacgggccgc aagaccttgc tgcttgatgt
     3241 ttacaatccg catcagccgc gggaattgcg gataaaggcc atcgagcggc attggcagcc
     3301 cacgcgtcat gagtttgtga cggaattcga ttcaccgagt gaggatccat cgggctggcg
     3361 cacgttacgt cttgacgccg ctcagtttca ggacaacgag ggacagtcca tggcggattg
     3421 gggcgcagtt gactgcctgc tgatcgaagg gatcacctcc gccgagcact cacccgtctt
     3481 caggcggctg cgttgggaga tggactcatc ggaggggaaa gcaccggcgc catccccgtg
     3541 atggccgtct actttcgcgc gcccgttcta atggtaggcg ggccaggtgc gactgatcgt
     3601 ttcaggcggc gggggagtgg gcgcgattga gcagacttga acgtgcgggt taagcacggc
     3661 agccatctcg ttcatctctt ccttcgtgcc taccttgagg aaatgctggc ggaaaacttt
     3721 gccaaacggt tcgatcaggg tccaacggtt ggttgccatg acggccgggg cgttcggata
     3781 attataagag gggaagatgc tgatgctgct ggagccatgg gtggtatggc caatgtagaa
     3841 ggcaattcca atgtccggcc gcacggtatc cacaaagtag ccggcccaac cgcccatttc
     3901 ccaaccatac aggctcaaac cagccggatt ggccccaccc gcaaagggct cgacgaattg
     3961 gccaccggcg ctggtgtaga aaacccggcc gttcggagcg ctgtcgaacg caatccagtg
     4021 cccatccgtt gcgtagggaa tccacgggcg gccctgcaga tcgctgaaga gatgcgccag
     4081 catgatggtg ccatcgggat agatgcggat cgcaatttcc attcgcacat tgtcgagggc
     4141 cgccgcgatc tgttgctgga tgcgcagcgc tgtctccatc ggataggtta cggcaaaatc
     4201 gttgagaaac gcattgcttt caggatcaag gatcccctca tagaatatcc agggaacgag
     4261 gaccgcggcg gcgcgcaatg tttgcgtcgc ttcgtcccag gccggaggac tttcaatttc
     4321 gggcaagatg ccgcgaccgc tgccggcgcc ctggtaattg tcgcagggcc agggctgaga
     4381 ataaaacgag gtgccacacc catagttggt gcggaccatc gtgttggtca tgccgatctc
     4441 ggttccgtaa ttcgggtgga gtccaatggc gggttggatc acccggtcca tggccgtcac
     4501 ctggccgccg gggcattcgg aataatgaaa tccgccgaca tattgagctt tttggtctga
     4561 actatccacc cggtgaaatt tccaaattgc cagcgaagga gaaaaataga ggtcgaaatc
     4621 ccgatccgct ggctccggtt cacggtagcg aatgactcca tagtgctcca accattcctg
     4681 aggcgcaccg ggagccgcga actccgcccc aaattcgcat gtggcgctcc ccctggatgc
     4741 gaaaagatcg gtaggaggcg ccttctcttc cgcttccggc caactccaac cacacaggag
     4801 gatcgctccg ccgatcaagg acaggatcag cagacggcgc gaatctcgcg tagatccgaa
     4861 gtgatcagga tttgtggact tcatatcaat tgcctcgctg tagcgcctgc gagtgtcgca
     4921 cgggcaattg gtttttcaac ggcctgccaa gcatagtcat tttcgacggg cgacgctctt
     4981 gagagccggc ttttgtttgc tcttggtgct gtcacgcacg atcatgctgg gtttgagcga
     5041 aacttggacg ctggggtgaa tcttgttctc gatggcatga ataagcattg tggtagccat
     5101 ctccgccaat tggtcgaagg gctggcggat ggaagtcaag gatatgcgag acaaatgcaa
     5161 tatatccagg tcctcgactc ccatcaccgc tacgtccctg ccaacttgta tcccaagctg
     5221 atccgccgcc tcaatgaccc ccaatgcaca gaggctgtta atgcagaaga tgccatcgac
     5281 atcgggaatc gtttccaaga cccggccgaa ggcagcctgg cccgcagcga tggtcaaacc
     5341 agcataaatg atctgctttg cgtcaaaggg cagcccctgc tcacgaatag agcaggcaaa
     5401 ttctcgaccc agaatatcga atgcactaaa gtgctccata tcccgatccg ccgtcaggaa
     5461 gaccggatac cgacacccga catcttgtgt caggtgcttg gccgcaagcc gcgccgcttc
     5521 gaccagatcg tttgcaacaa atgcctttgt gtaatcactt ggcacggtgt caaggaggat
     5581 atacgggaca agaaacctct cgagcaagcg caggctctca ggagtcctct cgcacagaca
     5641 ggccaggatt ccatcgacgc gcgattcgag cagatactcg attcccgcca gctccttgtc
     5701 cgcctgccag caactgtcgg ctaccaggat ggtgtaaccg tgagacatgg cgatggtttg
     5761 cgccgctcgc accatgagcg aataaaacgg gtgggtaatg tcgttgacga ggaaaccgat
     5821 gattttgttc ttggtgtgac cgcggcgcag gcgacgggct gccaggttgg gaatatagtc
     5881 gagctctttg gctttttcca gaacccgctg gcgggtcttg tcgctgatgg gagaattgtt
     5941 ttgaaatgcg agagaaacag tcgtttccga gacatttaac agtctcgcca aatctctgat
     6001 ggttaccttc atgctccgca acgaccctca tggcaggaag atctaaactc ttaacatggg
     6061 cattgcccta cttgtccagt aaaaacgtca aaacccaaac cgtcgggtcg ccaaggcttc
     6121 tcccagttga cggtgacgat gccgtgggcg gagcccggct cgggttctac ggcgatgttt
     6181 gaggcgttgg tgatcgaccg gctgtgcgcg gcggcggtct cggcggtggg aaggcggctg
     6241 gggctgtcct ggagcgtgtc gacgcgatca tgcagcaggc gtgctgcgcc gggggtgggc
     6301 gcggggcgag aag
//
LOCUS       DAOPCO010000235         6310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10875_length_6310_cov_4.796643, whole genome shotgun sequence.
ACCESSION   DAOPCO010000235 DAOPCO010000000
VERSION     DAOPCO010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10875_length_6310_cov_4.796643"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLS90_13630"
     CDS             <1..771
                     /locus_tag="PLS90_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HPK03484.1"
                     /translation="AGASEVVVMRLYGPLARQGHSVVTPMLLADSPVVAWWPREAPAD
                     LRTDPIAQLASRRITDAAESSRPGLMLARSAEHHRPGDTDLAWTRITLWRALLAAALD
                     GPPYERVVAASVSGAPDSPSTDLLAAWLAWALKVPVTRKHTPAGSGIHGVQLERPGGA
                     IDLVRPGDYVATMTQPGQPERRIGLRRRGDAECLADELRRLDPDETYADVLRKGLPLL
                     GRPRGAARPREVSETRRAEDRTARNRSVQLASTMGKRS"
     gene            875..1342
                     /locus_tag="PLS90_13635"
     CDS             875..1342
                     /locus_tag="PLS90_13635"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPK03485.1"
                     /translation="MRNGSYHRLSPDPLKPEYVSGRFGRLEKLLFTDTEIDQENKTRT
                     NPELVAYYKDRKNDLDDRRKAYKEYIRRFVFPAACFVFALIGVPISIWVRPSGKSWGI
                     LLAIGLMLIYYVLMQWGLSMVEDGKQLGLAMSIFPVALFSVLGALLWWHTLRS"
     gene            1447..4227
                     /locus_tag="PLS90_13640"
     CDS             1447..4227
                     /locus_tag="PLS90_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03486.1"
                     /translation="MLAVWLMAAAVGWSQDDLTRQPALDEGEAQRMTLGAAGTPPITF
                     DTVTIPPSIQRDRQAALRQTLRPITQPEAALRMRALEDVIDYDEIDQILYAPGRTQLE
                     YGQYYLEADKVIVDARLQEIQAEGNVLLRIRQDVGPPNDIYADSLRFNFREGEGVAYN
                     VSGQHGFIFFRNAAREGDLPPNTPQLQKLSEQESIFRHTSLTTCDFTIPHYSVTGREI
                     ILYERDRVFIRSATFRVWGVPVFYLPAYSRSLLESSPWFVQVGWGSRTGPRIRVGYGY
                     RHKTMEPSFKQDDLLEERSGGRADVYMDYLSEYGPGAGFDYEYQFAYNKHRGRMSLYG
                     LHDGDREVAGTQLLNPEEIFDLTGQVQNDNLQSEPNRWRVLWQHRSEITDELTFRLNI
                     DEFSDPDIFYDILDNFGRLDEERDRYVLRRSRAALSFVNEVLAIHLLADLKDRIGIDR
                     YTDFSRPSDDNRQFDLQPDRRLEDVDSDGISADRWAAASRRLPQVDIQTRWLPIGNRP
                     LYYLTELYLYNNLDKGFNSLNQRDDAYVRGAEIYQALLYQWKLSERYTLLAQAGIGVG
                     VADRENHDLDPTYRTTTIAAPTLADPLNTEEVVLFPNQVGGMVFIDDDGNFLVGRRTM
                     NMEDIEPTYVWGDAKLQFLARFSDALSGDLLWRIRETTDDFIGDWYASTGNLTFRDDL
                     FDYKIREHWVEGGLDYRLARPLINAFVRGGYNLVSRGDVFSKEDLAFARTGLGWSNQR
                     QTLAIRGSVGWEKDQQFDPSDIRAYEDEEIHLNAALSYSPIHGRWFIQLELERDIELD
                     QRSEVDDQKKLTFFTEDESQTEARLTFGRELGPKWKTEIEVEWDDEVGGLRELAWLLE
                     RDLHDAVAILRVEVENDEEDADDRETSASESPQIDVRLGLKFKIPGQNVAFGVEEVDT
                     IQREQRRAQLAY"
     gene            4269..5132
                     /locus_tag="PLS90_13645"
     CDS             4269..5132
                     /locus_tag="PLS90_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPK03487.1"
                     /translation="MSSHPEESIAVPPTGGLRVGVVAFLNMLPLVAGAERLAPGRLEI
                     VPAPPSRLAQWLAEGRVDLAMAPVAALFEHPEWRIVGRSMIGADGDVLSVLVVGREPR
                     ERWSALRPDGQSRTSNVLARLVLERRLGLRLPLGEPLPRDVDWEPPADPRPGEAFVLI
                     GDRALRWGNRWSTDAGGDRLDLGAHWKAWTGLPMVFAVWCARPGVVAGPWIEALEQLK
                     ELNRARLAELAAAWRRSGGDFLSPAEAENYLRQNLRFNLDAEALEGLRRFYAEGRSAG
                     LFADGWVLRRL"
     gene            5317..5610
                     /locus_tag="PLS90_13650"
     CDS             5317..5610
                     /locus_tag="PLS90_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03488.1"
                     /translation="MIRKLAGVWGLLLLPLLMTVACDVEKTREGNIDLPKYEVEQVEE
                     GDVTLPAYDVDLPDVDVNVRETTVTLPDIDIDVNERETTIPVPDIDINPPANP"
     gene            complement(5780..>6310)
                     /locus_tag="PLS90_13655"
     CDS             complement(5780..>6310)
                     /locus_tag="PLS90_13655"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPK03489.1"
                     /translation="QYVRFTLDAAPASWPDGRYPADQARLPVTGVTWHQAMNFCRWFA
                     ALGQLAPDSVRLPTLAEYRRALRGDTLRGDPTQTGYWARARLYQAAAPKTIKATPWDR
                     IYIPGSSQMYDLIGNVAEWGMDEQGANRVILGGDFSIDNTDFDHLQPRWRDPDQAYPE
                     VGFRLVHVIDATMPAP"
BASE COUNT         1050 a         2220 c         2023 g         1017 t
ORIGIN      
        1 gccggcgcga gcgaggtcgt cgtcatgcgg ctctacggcc cgctcgcgcg ccagggccac
       61 tcggtggtca cccccatgct gttggccgat tcccccgtcg tcgcctggtg gccacgcgag
      121 gccccggccg acctgcggac cgacccgatc gcgcagctgg cgtcccgccg gatcaccgac
      181 gccgccgagt cgtcccgccc cggcctcatg ctcgcccggt ccgccgagca ccaccggccc
      241 ggtgacaccg acctggcctg gacccggatc accctctggc gggccctgct cgcggccgcc
      301 ctcgacggtc cgccctacga gcgggtcgtg gccgccagcg tctccggtgc ccccgactcc
      361 ccctccaccg atctgctcgc ggcctggctg gcctgggcgc tcaaggtgcc ggtcacccgc
      421 aagcacaccc cggccggcag cggcatacac ggggtgcagc tcgagcggcc cggcggcgcc
      481 atcgacctgg tccgtccggg cgactacgtc gccacgatga cccagccggg ccagccggag
      541 cggcggatcg ggctgcgccg gcggggcgac gccgagtgcc tcgccgacga gttgcgccgc
      601 ctcgaccccg acgagaccta tgccgacgtc ctgcgcaagg gtctgccact gctcggccgc
      661 ccccgcggtg ccgcgcgccc gcgcgaggtg agcgagaccc ggcgcgccga ggaccgcacg
      721 gcccgcaacc ggtcggtgca gctggcctcg acgatgggga agcgttcgtg acctcggctg
      781 cggccggcgg cggcgggccg gtcgtgctcg tccacccgga cgcccgctcc gtcgcgacgg
      841 ccggcgcgac cgcaatgccg ccatcctcct ctcgctgcgc aacggctcct accaccggct
      901 ctcgcccgac ccgctcaagc cggaatacgt gtcgggccgg tttggccggc tcgaaaagct
      961 gctcttcacc gataccgaga tcgatcagga gaacaagacg cgcaccaatc cggagctggt
     1021 ggcgtactac aaggaccgca aaaacgacct ggatgaccgg cgcaaggcct acaaggaata
     1081 catccggcgc ttcgtcttcc cggcggcctg tttcgtcttc gcgctgatcg gtgtgccgat
     1141 ctcgatctgg gtccggccca gcggcaaatc gtggggtatt cttctcgcaa tcggcctgat
     1201 gcttatctac tatgtgctca tgcaatgggg cctgtcgatg gtcgaggacg gcaaacagct
     1261 cggcctggcg atgtcgatct tccccgtggc tcttttctcg gttctgggcg ccctgctctg
     1321 gtggcacact ctcaggagct gaagcagcat atgctcatgt tgaacggcag gcggcgactc
     1381 gggcgacggg ggtggctcct cccgttcccg gcggacggat cacgcgggcc cgggcgctgg
     1441 ctgccggtgc tcgccgtctg gctgatggcg gcggcggtcg gctggtcgca ggacgatctg
     1501 acccgccagc ccgcgctcga cgaaggcgaa gcccagcgca tgacgctggg cgccgccggc
     1561 acccccccca tcacgttcga cactgtcacg atccccccct cgatccagcg cgaccgccag
     1621 gccgccctgc gccagacgct ccggccgatc acgcagcccg aagccgcgct gcgcatgcgg
     1681 gcgctggagg acgtgatcga ctacgatgag atcgaccaga ttctctacgc cccgggccgc
     1741 acccagctcg aatacggcca gtactatctc gaagccgaca aggtgatcgt cgacgcgcgg
     1801 ctgcaggaga tccaggccga gggcaacgtc ctgctgcgca tccgccagga cgtcggcccg
     1861 cccaacgaca tttacgccga ctcgctgcgc ttcaacttcc gcgagggcga aggcgtcgcc
     1921 tataacgtca gcggccagca cggcttcatc ttctttcgca atgcggcccg ggaaggcgat
     1981 ctgccgccca atacgcccca gttgcagaaa ctgtcggagc aggaatcgat tttccgccat
     2041 acgtcgctga cgacctgcga cttcacgatc ccccattaca gcgtcaccgg ccgcgagatc
     2101 attctctacg aacgcgaccg cgtcttcatc cgcagcgcca catttcgcgt ctggggcgtc
     2161 cccgtgttct acctgcccgc ctactcgcgt tcgctgctcg aatcgagccc ctggttcgtc
     2221 caggtcggtt gggggtcgcg caccggaccg cgcatccgcg tcggctatgg ctatcgccac
     2281 aaaacgatgg agcccagctt caaacaggat gacctgctgg aggagcgctc gggcggccgc
     2341 gccgacgtct acatggacta cctgagcgaa tacggtccgg gcgccggctt cgattacgaa
     2401 taccagttcg cctacaacaa gcatcgcggc cgcatgtcgc tctacggtct gcacgacggc
     2461 gatcgcgaag tcgccggcac gcaactgctc aaccccgagg agatcttcga cctgaccggc
     2521 caggtgcaga acgacaacct gcaatcggaa cccaaccgct ggcgcgtcct gtggcagcac
     2581 cgcagcgaga tcaccgacga actgacgttc cggctgaaca tcgacgagtt cagcgacccc
     2641 gacattttct atgacattct ggacaacttc ggccggctgg acgaggagcg cgaccgctac
     2701 gttctgcgcc gcagccgcgc ggccctctcc ttcgtcaacg aagtgctggc gatccatctg
     2761 ctggccgacc tgaaggaccg catcggcatc gatcgctaca ccgatttttc gcgccccagc
     2821 gacgacaacc gccagttcga cctgcagccc gaccgccggc tcgaggatgt cgactcggac
     2881 ggcatcagcg ccgatcgctg ggccgccgcc agccgccgcc ttccccaggt cgatatccag
     2941 acccgctggc tccccatcgg caaccgcccg ctctactatt tgaccgagct ctacctctac
     3001 aacaacctgg ataagggctt caactcgctc aaccagcgcg acgacgccta cgtccgcggc
     3061 gccgagatct atcaggccct gctctaccag tggaagctga gcgagcgcta cacgctgctg
     3121 gcgcaggcgg gaatcggcgt cggcgtcgcc gaccgcgaaa accatgacct ggacccgacc
     3181 taccgcacga cgacgatcgc cgcgccgacg ctggcggacc cgctcaacac cgaagaagtc
     3241 gtccttttcc ccaaccaggt cggcggcatg gtcttcatcg atgacgacgg caacttcctc
     3301 gtcggccgcc ggacgatgaa catggaggac atcgagccga cctacgtctg gggcgacgcc
     3361 aagctgcagt ttcttgcccg cttcagcgac gccctgtcgg gcgacctgct ctggcgcatc
     3421 cgcgagacga ccgacgactt catcggcgat tggtatgcca gcaccggcaa cctgaccttc
     3481 cgcgacgatc tcttcgacta caagatccgc gaacactggg tggagggcgg tctggactat
     3541 cgcctggcgc gtccgctgat caatgccttc gtccgcggcg gatacaatct ggtcagtcgc
     3601 ggcgacgtct tctccaagga ggacctggca ttcgcccgca ccggcctggg ctggagcaac
     3661 cagcgccaaa cgctcgcgat tcgcggcagc gtcggctggg agaaggatca gcagtttgac
     3721 ccaagcgaca tccgcgccta cgaggacgag gagatccatc tcaacgcggc gctgagttac
     3781 tcgccgatcc atgggcggtg gttcatccag ctcgaactgg agcgcgacat tgaactggat
     3841 cagcgcagcg aggtggacga tcagaaaaag ctgacgttct tcaccgagga cgaatcgcag
     3901 accgaggcgc gcttgacgtt cggccgcgag ctgggaccca agtggaaaac cgaaatcgaa
     3961 gtcgagtggg acgacgaggt gggcggcctg cgcgagctcg cctggctgct cgagcgcgac
     4021 ctgcacgacg ccgtcgcgat cctgcgcgtc gaagtcgaga acgatgagga agatgccgac
     4081 gatcgcgaaa catcggcgtc cgaaagcccg cagatcgacg tccgcctcgg cctcaagttc
     4141 aaaatccccg gccagaacgt cgcgttcggc gtcgaggaag tcgatacgat tcagcgcgaa
     4201 cagcgccgcg cgcaactggc gtactgacgg cgccgccggc cccagcatca tcccaggtcc
     4261 accgttgcat gtcgtcccat cccgaggagt ccatcgccgt cccgcccacc ggcggcctgc
     4321 gggtgggcgt ggtcgcgttt ctcaacatgc tcccgctggt ggcgggcgcc gagcggctgg
     4381 cgccgggccg gctggagatc gtgccggcgc cgcccagccg actggcgcaa tggctcgccg
     4441 aggggcgcgt cgacctggcc atggcgcccg tggccgcgct gtttgaacac cccgaatggc
     4501 ggatcgtcgg ccggtcgatg atcggcgccg acggcgacgt cctcagcgtc ctggtcgtcg
     4561 gccgcgaacc gcgtgaacgc tggagcgcgc tgcggcccga cggtcagtcg cgcacgagca
     4621 atgtgctcgc ccggctggtg ctggaacggc gcctcggctt gcgactcccg ctgggcgaac
     4681 cgttgccgcg cgacgtcgat tgggaaccgc ccgccgaccc gcggccgggc gaagccttcg
     4741 tcctgatcgg cgaccgcgct ctgcgttggg gaaaccggtg gtcgaccgat gccggcggcg
     4801 accggctcga cctcggcgcg cactggaagg cgtggaccgg cctgccgatg gtgttcgccg
     4861 tctggtgcgc acgacccggc gtggtggccg gcccgtggat cgaagcgctg gaacaactca
     4921 aggagctcaa tcgcgcacgc ctggcggagc tggccgccgc ctggcgccgc tccggcggcg
     4981 atttcctttc gcccgcggag gcggaaaact acctgcgcca gaatctgcgc ttcaacctgg
     5041 atgcagaagc gctggaaggg ctgcggcggt tttacgccga ggggcgctcg gccggcctgt
     5101 tcgccgacgg ctgggttttg cgccggctgt gaggctttcc cgccccgcgc ctaagatctg
     5161 caatgccagc ggcccgggcg ccggacgggc cttcgccgca aggcgcctcg ccgagagctt
     5221 ttcgagcatc cttgccccgt tggattgatc cttgcttcat ctccctgaca actttgcacg
     5281 gtggcccgcg cggccatcga tagaaaggat ctggagatga ttcgcaagct cgctggggtc
     5341 tgggggttgc tcctgctccc cctgctgatg acggtcgcct gcgacgtcga gaaaacccgg
     5401 gagggaaata tcgatctccc caaatacgaa gtggaacagg tcgaggaagg cgacgtgacg
     5461 ttgcccgcct atgatgtgga tctgcccgac gtggacgtga acgtgcgcga aacgacggtc
     5521 acgctgcccg atattgatat cgatgtgaat gaacgcgaga cgacaattcc ggttcccgac
     5581 atcgacatca acccgccggc gaacccataa ccaacctgca attgacctgc cggggggttt
     5641 cgatgaactg acggcgggtg tcggtgctcc agatcaaccg gtcgctgagg tccgggctga
     5701 gcaccggcaa ccgccgcgct tcattcccgg tggatcggcg ggcaaccgct cgcctgttca
     5761 ggttgccgta tccgccggct cagggcgccg gcatcgttgc atcgatcacg tgcaccagcc
     5821 ggaagccgac ttcgggatag gcttggtccg ggtcccgcca gcgcggctgc agatggtcaa
     5881 agtccgtgtt gtcgatggag aagtcgccgc cgagaatcac gcggttcgcc ccctgctcgt
     5941 ccatgcccca ttcggcgacg ttgccgatca agtcgtacat ttgtgaagaa ccggggatat
     6001 aaattcgatc ccagggggtc gccttgatcg tcttgggcgc cgccgcctga tagagccgcg
     6061 cccgcgccca gtagccggtt tgcgtcggat cgccgcggag cgtatcgccg cgcagcgcgc
     6121 gccggtattc ggccagcgtg ggcagccgca ccgaatccgg ggcgagctgg cccagcgccg
     6181 cgaaccacct gcagaagttc atcgcctgat gccaggtgac gccggtgacc ggaagccgcg
     6241 cctggtcggc gggatagcgc ccgtcgggcc agcttgccgg cgcggcatcc agcgtgaaac
     6301 gcacatactg
//
LOCUS       DAOPCO010000236         6278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10930_length_6278_cov_4.914832, whole genome shotgun sequence.
ACCESSION   DAOPCO010000236 DAOPCO010000000
VERSION     DAOPCO010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10930_length_6278_cov_4.914832"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PLS90_13660"
     CDS             complement(<1..761)
                     /locus_tag="PLS90_13660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03490.1"
                     /translation="MKSSIFPDWIAALAKGRPLADEVAIVTGASRGIGRSVAETLAQA
                     GAVVALAARSAPELERMQQALSEGGGRVLAIPTDLERDSDITALAEITANDLGMPTIL
                     VNNAGIGLYGPLGQFPIRHWDRVMAVNARAPFILCRAVLPYMREVGRGTIINLASVVG
                     IKGYVNQGAYSAAKHALMGMSKVLAQEVQADGIRVHTLCPGGVDTEMAWLSRPDLKRE
                     ELIQPMEIAGLILFLLRTRDGRAVIDDLHIRRASGS"
     gene            1795..2808
                     /gene="lgt"
                     /locus_tag="PLS90_13665"
     CDS             1795..2808
                     /gene="lgt"
                     /locus_tag="PLS90_13665"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPK03491.1"
                     /translation="MHPVLFRFGNLTIGTYGLMLVVGMLAGLTLASFVARRRGLRADF
                     FWDLAFVLLVSGLLGARLLYLAVNWDDFLRAPAALLLARQGFVFQGGFLVALVAGIAF
                     ILWRRMPLFEVGDVLAPGLALAHGIGRIGCFLAGCCYGRVCTPGQASAWMQALAVRYP
                     LILDAHGIPDGMFNFAFRDQLQAGLLDGGAPASLPLFPVQLFEAAGNFLICLLLLAAW
                     RGKRFSGQIFGLYLVAYSLLRFGLEYLRGDRERGLWFDDLLSTAQLISIATLIAGLIF
                     LLVRSFHGLETIPATARPPVGEPAAPPPAQSGPDAAAPVELPAAGPRRARRNRRHPRR
                     AAP"
     gene            complement(2809..>3417)
                     /locus_tag="PLS90_13670"
     CDS             complement(2809..>3417)
                     /locus_tag="PLS90_13670"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HPK03492.1"
                     /translation="PPREPDRSIYRDYPARAEDYVAVFAERFAALRGEFLRAAGLAEA
                     AARVAGLLAELEASEACGAARGLAAADPLIAAVLAASPALAARFDPPERLGMANAEML
                     PCLAGLTRARALVARSGGIVLDSGRDGGRRLSVFPPLHIVLARAGDYVASLEEALARR
                     AGDADWSSLTIVHGPSRTADIEKTLVLGAHGPKRLAVIHVED"
     assembly_gap    3418..3517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3628..4992)
                     /locus_tag="PLS90_13675"
     CDS             complement(3628..4992)
                     /locus_tag="PLS90_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018476132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HPK03493.1"
                     /translation="MTARLREFRRAADERAADLVHRERIARVMSKSNAASETMKVRQF
                     GDWERARRLAGQIKRHVLANLPDLLEEFERNATARGVEVLWAADAGEARALLLGLARR
                     FQIEIAVKSKSMTTEEIELNAALEAAGIEVLESDLGELIVQLAGTRPYHIVGPAIHLT
                     RAEIAELFHRKLGTAPGAGAEELTLAARDLLRRKFAAAGLGITGANFLLADAGAITVV
                     ENEGNARLTMAMPRVHVAIAGIEKVLPRLADLALFLPLLASSGTGQQLTCYNTLARGP
                     RTAGEPDGPEHMAVILLDNGRTTLYARPAVRQALRCIRCGACLNVCPVYRSIGGYTYG
                     TVYSGPIGAVITPHLEEQGRWEHLSQASSLCGACSAACPVDIDLHRLLLENRRRATAE
                     RKEGWFWAAALRAWAWVFSRRGRLDRLRGVGRLVRAAAPGRARVPRLAPRSFAQMWSD
                     REQS"
     gene            complement(4989..5753)
                     /locus_tag="PLS90_13680"
     CDS             complement(4989..5753)
                     /locus_tag="PLS90_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPK03494.1"
                     /translation="MAPSTVSVLIPCLVDQVYPEMGWAMVAVLRRLGYGVDYRRGQTC
                     CGQPAFNAGHWGEARRVAGHCLEVFRGAERVVCPSGSCTAMLREFYAELFAGDAREAE
                     AAALAGRVEEFSEFLLREGAAGRLGARLPGRLGFHNSCHSRRELGQRDESRRVLEQLE
                     GCEVVEPLGEPDCCGFGGLFSVKFAGIAGAMAQSRLARFHELGVARLAVNDPGCILHL
                     RQEAERAGLALEIRHVAEWVAEALGVAAAPGAGAER"
     gene            complement(6048..>6278)
                     /locus_tag="PLS90_13685"
     CDS             complement(6048..>6278)
                     /locus_tag="PLS90_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03495.1"
                     /translation="RAQEDGFEGVDGVGEGGEVGVGHATEENTKYEFRSTKQIRNGKR
                     RDGGGGSRRWTPTDGGAGAARWGRLYKGEEEK"
BASE COUNT          898 a         2529 c         1749 g         1002 t
ORIGIN      
        1 gaccccgacg cccgtcggat atgcagatca tcgatcaccg cgcgcccgtc gcgcgtccgc
       61 agcaggaaca ggatcaaccc cgcgatctcc atcggctgga tcagctcctc ccgcttcagg
      121 tccggccgcg acagccatgc catctccgta tcaacccccc cgggacacag cgtatgcacc
      181 cggatcccgt cggcctggac ctcctgcgcc agcactttcg acatccccat cagcgcgtgc
      241 tttgccgcgc tgtaggcccc ctgattcaca tatcccttga tccccacgac cgacgccaga
      301 ttgatgatcg tcccgcgccc cacctcccgc atatacggta gcaccgcccg gcacaggatg
      361 aacggcgccc gcgcattgac cgccatcacc cggtcccagt gccggatcgg aaactgcccc
      421 agcggcccgt aaagcccgat ccccgcattg ttcaccagaa tcgtcggcat ccccaggtcg
      481 ttcgccgtga tctcggccag cgcggtgatg tcgctgtcgc gctccaggtc ggtcgggatc
      541 gccaggaccc gaccgcctcc ttcactcagc gcctgctgca tccgctccag ctccggcgcc
      601 gagcgcgccg ccagcgccac caccgcgccc gcctgggcca gcgtctcggc taccgaccgc
      661 ccgatgcccc ggctggcgcc cgtcacgatc gccacctcgt cggccagcgg ccgacccttc
      721 gccaacgccg caatccaatc aggaaaaatg gaggatttca tccgcttggt cccgaaatga
      781 gaattccaag aggttcattc ggagagtcac cctggcccga ctgtgtcaga ataagcgaca
      841 ggatgacgca ttgccagcaa agaaacgcct tccccgcagc caaacaaaag attttgagcg
      901 cctaaaaacc gattctgcag tggcgaaatc acgactccct gatcggcaac tcccccttcg
      961 gcagtatcaa atcgtgcctc ttttcacaga ctgttgtgtg caggtggctc atattgatct
     1021 ctcgttttga gaaaccagcc gctcccgatt atccttcatc gccattcata ccgctgaaaa
     1081 tacttgcctt ataataaaca tgggccctca ttcatcatcc tggcacggtt ctcgctccct
     1141 cattggggca tctggatagg aatcgaagtc gtccaccaga tcggttcaaa aaaccgagcc
     1201 ggatcaagga gttggaacgg aacctgatat aagattttca aagcacgatc gaggcgcagg
     1261 ggctgcctac cagaagtgag atcctaccac caacgaagag ccaaccttgt taatctccag
     1321 gctggcacag cccagccgaa agcccctcct ttggatactg acctcgcggt cagttccact
     1381 ttcaaccctg cgcctcggtc tgccgatttt caagaagtga tctgagaagc ataatccaag
     1441 cacttccccc tttcacctga aaatccccgc tcacccgggg atttttttac gtctcccccc
     1501 ccgataattc tgtctcccct tttcgtccct catcccttgt cctcgtgatc gcccccaacg
     1561 gccgccccag atttcggctc tcggctctcg tcctcggctc tcggctctcg aaaaccgcac
     1621 gccccgcgcc gcggaccgca ccccgcaacc acgccgcgcc tcccgccatc ccgccgcccc
     1681 gccccgggtg ttcttgcgtg acatgtcgcc cgtttcctgt gaaggtagag cggcgggggg
     1741 cgcacctccg ccgcccgact ccatcccacc cgccccggcg acgacgaccc gcgcatgcac
     1801 ccggttctct ttcggttcgg caacctgacg atcggcacct acggcctgat gctcgtcgtc
     1861 ggcatgctgg ccggcctgac gctggcctcc ttcgtcgcgc gccgtcgcgg cctgcgcgcc
     1921 gactttttct gggatctcgc cttcgtcctc ctcgtcagcg ggctgctcgg cgcccgcctg
     1981 ctctacctcg ccgtcaactg ggacgatttc ctgcgcgcgc ccgccgcgct cctcctcgcg
     2041 cgccagggct ttgtcttcca gggcggcttc ctcgtcgccc tcgtcgccgg catcgccttc
     2101 atcctctggc gccgcatgcc gctgttcgaa gtgggcgacg tcctcgcgcc cggcctggcg
     2161 ctggctcacg gcatcggccg catcggctgc ttcctcgccg gctgctgcta cggccgcgtc
     2221 tgcacccccg gtcaggcctc ggcctggatg caggcgctgg cggtccgcta cccgctgatc
     2281 ctcgacgccc acggcatccc cgacggcatg ttcaacttcg ccttccgcga ccagctccag
     2341 gccggcctgc tcgacggcgg cgcccccgcc tcgctccccc tcttcccggt tcagcttttc
     2401 gaggccgccg gcaatttcct catctgcctg ctgctgctcg ccgcatggcg cggcaagcgc
     2461 ttctccggtc agatcttcgg cctctatctg gttgcctaca gcctgctgcg cttcggcctg
     2521 gagtatctgc gcggcgaccg cgagcgcggc ctgtggttcg atgatctcct ctccacggct
     2581 caactcatca gcatcgccac gttgatcgcc ggcctcatct tcctcctggt tcgcagcttt
     2641 cacggcctgg aaaccatccc cgcgacggcg cggccgccgg tcggcgaacc cgccgccccg
     2701 cccccggccc agtccggtcc ggatgccgcc gcgccggtcg agctgcccgc cgccggcccg
     2761 cgccgcgccc ggcgcaaccg gcgtcacccg cgccgcgccg ccccctgatc aatcctccac
     2821 gtgaatcacc gccagccgct tcgggccgtg cgcgcccagc accagcgtct tttcaatgtc
     2881 ggccgtgcgc gacgggccgt ggacgatcgt caggctgctc cagtccgcgt cgcccgcccg
     2941 ccgcgccagc gcctcctcca gcgaggccac gtagtccccc gcccgggcca gcacgatgtg
     3001 cagcggcggg aagaccgaca gccgccggcc gccgtcgcgc ccgctgtcga gcacgatccc
     3061 cccgctgcgc gccaccagcg cccgcgcccg cgtcagcccc gccaggcagg gcagcatttc
     3121 ggcgttggcc atgcccaggc gttcgggcgg gtcgaaccgc gccgccagcg ccggcgacgc
     3181 cgccagcacc gcggcgatca gcgggtcggc cgccgccagc ccgcgcgccg cgccgcacgc
     3241 ctcgctcgcc tccagctcgg ccagcaggcc ggccacccgc gccgcggcct cggccagccc
     3301 cgccgcccgc agaaactccc cccgcagcgc cgcgaaccgc tcggcgaaca ccgcgacgta
     3361 gtcctccgcc cgcgccgggt agtcgcggta gatcgagcgg tccggctcgc gcggcggnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct ccgcccgcgc cgggtagtcg
     3541 cggtagatcg agcggtccgg ctcgcgcggc ggcgccgctc gctccgccgc cggcggctgc
     3601 gccgcccgca accggcccag gattcgttca cgactgctca cgatcgctcc acatctgcgc
     3661 gaaactgcgc ggcgccagcc gcggcacgcg cgcccgcccc ggcgccgccg cccgcaccag
     3721 ccggcccacg ccccgcagcc ggtccagccg tccgcggcgc gaaaacaccc aggcccacgc
     3781 ccgcagcgcc gccgcccaga accagccctc cttgcgctcg gcggtcgccc gccgccggtt
     3841 ctccagcagc agccggtgca ggtcgatgtc gaccgggcag gccgcgctgc acgccccgca
     3901 caggctcgag gcctgcgaca ggtgctccca gcgcccctgc tcctccaggt gcggcgtaat
     3961 caccgcgccg atcgggcccg agtagaccgt gccgtacgta tacccgccaa tcgaacggta
     4021 gaccgggcag acgttcaggc acgcgccgca gcgaatgcag cgcagcgcct ggcgcaccgc
     4081 cggcctggcg tacagcgtcg tccgcccatt atcgagcagg atcaccgcca tgtgttcggg
     4141 gccgtcgggc tcgcccgccg tgcgcggccc gcgcgccagc gtgttgtagc acgtcagttg
     4201 ctggccggtg ccgctgctgg ccagcagcgg cagaaacagc gccaggtcgg ccagccgcgg
     4261 cagcaccttt tcaatccccg cgatcgccac atgcacccgc ggcatcgcca tcgtcagccg
     4321 cgcgttgccc tcgttttcga cgacggtgat cgcccccgcg tcggccagca ggaagttcgc
     4381 gccggtgatg cccaggccgg cggcggcgaa tttgcgccgc aacaggtcgc gcgccgccag
     4441 cgtcagctcc tccgcccccg ccccgggcgc cgtccccagc ttgcggtgaa acagctcggc
     4501 gatctccgcg cgcgtcaggt ggatcgccgg cccgacgatg tgatacggcc gcgtccccgc
     4561 cagttgcaca atcagctcgc ccaggtcgct ctccagcacc tcgatgccgg ccgcttcgag
     4621 cgccgcattc agctcgattt cctcggtcgt catcgacttg gatttgacgg cgatctcgat
     4681 ctggaaccgc cgcgccagcc ccagcagcag cgcgcgcgcc tcgcccgcgt cggccgccca
     4741 cagcacctcc acgccgcgcg ccgtcgcgtt gcgctcgaac tcctccagca ggtcgggcag
     4801 attggccagc acgtggcgct tgatctgtcc cgccagccgc cgcgcccgct cccagtcgcc
     4861 gaactggcgc accttcatcg tctccgacgc cgcgttcgac ttgctcatca cgcgcgcaat
     4921 ccgctcgcgg tggaccaggt cggccgcgcg ctcgtccgcg gcgcgccgga actcgcgcag
     4981 ccgcgccgtc atcgctccgc ccccgcgccc ggcgccgccg ccacccccag cgcctcggcc
     5041 acccactccg ccacgtggcg gatctccagc gccagccccg cgcgctccgc ttcctggcgc
     5101 aggtgcagga tgcagcccgg gtcgttcacc gccagccgcg cgacgcccag ttcgtggaac
     5161 cgcgccagcc gcgactgcgc catcgctccg gcgatccccg cgaatttgac cgaaaacagc
     5221 ccgccgaacc cgcagcagtc cggctcgccc aatggctcga ccacctcgca gccctcgagc
     5281 tgctccagca cccggcgcga ttcgtcgcgc tggcccagct cgcgccggct gtggcacgag
     5341 ttgtggaacc ccagccggcc cggcaggcgc gcgcccagcc gccccgcggc cccctcccgc
     5401 agcaggaact cgctgaactc ctccacgcgg cccgccagcg ccgccgcttc ggcctcgcgc
     5461 gcgtcgccgg cgaacagctc cgcgtagaac tcgcgcagca tcgccgtgca cgaccccgac
     5521 gggcagacca cccgctccgc cccgcggaag acctccaggc agtggccggc aacgcgacgc
     5581 gcctcccccc agtggccggc gttgaacgcc ggctgcccgc agcaggtctg cccgcgccgg
     5641 tagtccacgc cgtaacccag ccggcgcagc accgccacca tcgcccagcc catttcgggg
     5701 tagacctggt cgaccagaca ggggatgagc accgagaccg tggaaggcgc catgaaggaa
     5761 cggccgcccg cgaagattac ggcggcccgc ggaggatgcc ggcggcccga tcttaccccg
     5821 ccccccgccg cggtttcaat gcgtcatgga cttcgggaac gggaagcaag gggcaagggc
     5881 gaaggggatg gcgatcgcga tcccgaccag ccgtcctgct tctctgtgag acctttcgct
     5941 ctttgtggga ttctcgacct ctttcgtgcc ccttcgtctc tctcgtgatc cacaatcccc
     6001 caaaagattt tgtccccccg gattccccta agggtccctg agggaattca tttctcttcc
     6061 tcccccttgt ataatcttcc ccagcgggcc gcccccgccc cgccgtctgt cggcgtccac
     6121 ctccgcgaac caccaccgcc gtctcgcctc ttcccatttc gaatctgttt cgtacttcga
     6181 aattcgtatt tcgtattttc ttccgtcgca tgtccaaccc caacctcgcc accctctccc
     6241 acgccatcga cgccttcgaa gccgtcctcc tgcgctcg
//
LOCUS       DAOPCO010000237         6265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_10958_length_6265_cov_4.311916, whole genome shotgun sequence.
ACCESSION   DAOPCO010000237 DAOPCO010000000
VERSION     DAOPCO010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10958_length_6265_cov_4.311916"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..970)
                     /locus_tag="PLS90_13690"
     CDS             complement(59..970)
                     /locus_tag="PLS90_13690"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPK03496.1"
                     /translation="MAQPHSLLQAVRERVLLLDGAMGTQLQAVGLRPEQCGEAWNLQE
                     RDKVLGIHRRYVEAGADALITNSFGASSYGLAHRGFADQAYAINRASAEIAREAIGPD
                     GWVLGDVGPFGHFLQPLGEMTAERLLTIFTEQIRGLLDGGVDAILLETHTALEEAEIA
                     VRAARDQGAPCIIVSFAYNKGGGSYRSIMGAAPADVVARLDPLGVEIYGANCGTHLTV
                     EDHAEIVKAYRAATDKPIIAQPNAGQPQMEGGCLTYLETPEKMAERIGRLVEAGARLV
                     GGCCGTTPDHIRAFRKVLDQYSSVRVQ"
     gene            1096..1863
                     /locus_tag="PLS90_13695"
     CDS             1096..1863
                     /locus_tag="PLS90_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03497.1"
                     /translation="MPDPRFDLTGRTILITGSTRGLGWGMARGLAEAGARVVLNGTNQ
                     ELLRARVEELTAAGAEARAAAFDVRDEEGVGRAIAEIAASWGPLDGVVNNAGINRRGP
                     LLDFAPEDFQAVLDINLLGMWRVARHAARGMIARRRGKIINIGSITEFGGRATIGPYI
                     AAKAAVSALTRTMAVEWGPHNIQVNAIAPGYFETDLNRVLKEDAQFDAWVRSKTPAER
                     WGQPEELIGIAIFLASRASDYVNGQTIRVDGGWTAKL"
     gene            complement(1916..2779)
                     /locus_tag="PLS90_13700"
     CDS             complement(1916..2779)
                     /locus_tag="PLS90_13700"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPK03498.1"
                     /translation="MLIDYHSHTWRCRHAHGAMEQYVQRAIALGFSEFGFSDHSPWMI
                     QARGEHVAMAPEELPDYVADVQALRRRFNSDQFTLRLGLEMDYVPSRRAIARRAIEQF
                     PWDYLIGSVHHLGFWGLPKPSHAHFFETARIEDVCELYYHLVGEMIDEGFCDIIAHLD
                     LPGKFGHFPPGGMVPYVEPLIPRMLARGTAVEINTSGVDAPCAEVQPGWDVLALLAEA
                     HVPLLVNSDAHQPEHIGRHFPETLSRLRDLGVRTLTRFEARRRIEYPLPEPGEIARLE
                     RALQSPQADLG"
     gene            complement(2870..4984)
                     /locus_tag="PLS90_13705"
     CDS             complement(2870..4984)
                     /locus_tag="PLS90_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK03499.1"
                     /translation="MPTLTVISLTRNRFHRLADCLQRVLGQLEPGDEMLVIDTGSTDA
                     TRAHYAHWQHPRFRFLAHDDGGSWAEIRNRGVREARGDLIAFLDDDCMAAPDWVARGK
                     ADLTTADAVGGIARPWGIRRLPGWWHPDMAWMVGLSVPGMVGPDAGRLHYPMTANLWV
                     HAAVAREFPFQEIGGRLGGKVAEHYVAGREDAQWWATLRRAGCRTRIDPQLWVGHAID
                     PARLALPYLRERARLDGAAWAEREGTPDDLDHLAYAWWDHHLRAFAAPFREPDNLPAA
                     WHYHQLMRRRHAAALVALTSRLGRRPRLIARAAVLARLARTGPRYLADRGRTAVHALI
                     RPWIVSRPRRPRALVIERVAVIALGYLGDLVLLHCVLRGIMRENPRLGVYVLAPPAAR
                     TALGELPRLNLTLLPSLPPESREARDWLLRWLERIDPDAIVAPYLHGPWGVALASIRH
                     PPRPIFAFDRDQGLRRRLHRERLGVRVHKHLELHEIDNLAHLMRVAGLAGDPSPALIR
                     PPADAAERVARHPLMARPGPLVMLNPAAAHAQKEWTEERWAALLARLLAETDARVVIN
                     AFAPLRDLERAAEQAGERTLLLRAAPLPELVAWLERCHGLVTVDGGPQHLAHALDIPS
                     ITLYGPMDERRWADYWERPIHRTIRACTFDLTPDERRGLPENHMVALIPLEDVWRPLK
                     ELLDRWPREERRPVLTPPPASP"
     gene            complement(5098..5943)
                     /locus_tag="PLS90_13710"
     CDS             complement(5098..5943)
                     /locus_tag="PLS90_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPK03500.1"
                     /translation="MTRILMFGLILAAGCAPAPAGAAEHGRLLLIGQPTLWLEPGPGE
                     PNGPPMNWKAGTELTVFADCPLEAAHGETWFQVGQDAPAGWLPEACLSLPPVDFDESS
                     LRAIGQARVDRTAGLPPNYAPDDLVSVGPRYEQDRDYKLRAEAAEALAAMLAAAKADG
                     HTLYVVSGYRPWSVQQTLYERKVKAAGRDQKTVAKPGHSEHQLGTAVDLTDGNEETLL
                     RVSFGESPAGRWLREHAWEFGFAQSYTEHNEAETGYQPEPWHFRYWGIDQARTKHLRA
                     LGVEE"
     gene            complement(6044..>6265)
                     /locus_tag="PLS90_13715"
     CDS             complement(6044..>6265)
                     /locus_tag="PLS90_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPK03501.1"
                     /translation="RLDLDLLLFGSVRRDAPGLTLPHPWIARRDFVLAPLIDLQVPPL
                     PAIAPAGWGALLAALPTGERAILRAERWP"
BASE COUNT          962 a         1895 c         2510 g          898 t
ORIGIN      
        1 agccgagagc cgaattttgg ggcgggccgt tgggggcgag gaggaggggc gaacccgctt
       61 actggacgcg cacggaggag tactgatcga ggaccttgcg gaaggcgcgg atgtgatcgg
      121 gcgtggtgcc gcagcagccg ccgacgagcc gcgcgccggc ctcgaccagc cgtccgatgc
      181 gctcggccat cttttcgggg gtttccagat acgtcaggca gccgccctcc atctggggct
      241 ggccggcatt gggctgggcg atgatcggtt tgtcggtggc ggcgcgatag gctttgacga
      301 tttcggcgtg gtcctcgacc gtcaggtggg tgccgcaatt ggcgccgtag atctcgacgc
      361 cgagggggtc gaggcgggcg acgacatcgg ccggcgccgc gcccatgatc gagcggtagc
      421 tgccgccccc cttgttatag gcaaacgaga cgatgatgca gggggcgccc tggtcgcggg
      481 cggcgcggac ggcgatctcg gcttcctcga gcgcggtgtg ggtctcgagc aggatggcgt
      541 cgacgccgcc gtcgaggagg ccgcggatct gttcggtgaa gatcgtcagc aggcgttcgg
      601 cggtcatctc gccgagcggc tggaggaagt ggccgaaggg gccgacatcg ccgaggaccc
      661 agccgtcggg gccgatggcc tcgcgggcga tctcggccga ggcgcgattg atggcgtaag
      721 cctgatcggc gaagccgcga tgggcgaggc cgtaactgga agcgccaaag gaattggtga
      781 tgagggcgtc ggcgccggct tcgacataac gacggtggat gccgagaacc ttgtcgcgtt
      841 cctggaggtt ccaggcctcg ccgcactgct cggggcggag gccgaccgcc tgcaactggg
      901 tgcccatggc gccgtcgagg agcaggacac gctcgcgcac ggcttgcagc agagagtggg
      961 gttgggccat ggccaggctt cctttccgct gtcggggtgc tgtaagatgc ggccgatcct
     1021 aaccgaccgg gagcggaaaa tcaagacgaa cagatccgct ttgttgtccc gaatccgcag
     1081 acgaaagacc gcgccatgcc cgaccctcgc ttcgacttga ccggccgaac gattctgatc
     1141 accggctcga cccgcggact gggttggggg atggcgcggg gcctggccga agccggggcg
     1201 cgcgtcgtgc tcaatggaac gaatcaggag ctgctgcggg cgcgcgtgga ggaactgacg
     1261 gcggcggggg cggaggcgcg ggcggcggcg ttcgatgtgc gcgacgagga aggggtcggc
     1321 cgcgcgatcg ccgagatcgc ggcgagctgg gggccgctgg acggagtggt caacaacgcc
     1381 gggatcaatc ggcgggggcc gttgctggac ttcgcgccag aggatttcca ggcggtgctc
     1441 gacatcaacc tgctggggat gtggcgggtg gcgcggcatg cggcgcgggg gatgatcgcg
     1501 cggcggcgcg gcaagatcat caacatcggc tcaatcacgg agttcggcgg acgcgcgacg
     1561 atcgggccgt acatcgccgc gaaggcggcg gtctcggcgt tgacgcggac gatggcggtg
     1621 gaatggggac cgcacaacat ccaggtgaac gcgatcgcgc cgggctattt cgagaccgac
     1681 ctgaaccgcg tgctgaagga ggacgcgcag ttcgacgcgt gggtgcgctc caagacgccg
     1741 gccgagcggt ggggtcagcc cgaggaactg atcggaatcg cgatctttct ggcctcgagg
     1801 gcgtcggatt acgtgaacgg ccagacgatc cgcgtcgatg ggggctggac ggccaaatta
     1861 tagcggcaaa atggaaccgg cggcgcgccg cgcgcatggg cggcgaccgc gctgattatc
     1921 ccaggtccgc ctggggcgat tggagcgccc gctcgagtcg ggcgatttcg cccggttcgg
     1981 ggagcgggta ttcgatgcgc cggcgcgcct cgaagcgcgt gagcgtgcgg acgcccagat
     2041 cgcgcaggcg ggacaaggtt tcgggaaaat ggcggccgat atgctcgggc tggtgggcat
     2101 cggagttgac gagcaggggg acgtgggcct cggccagcag cgccagcaca tcccagccgg
     2161 gctggacctc ggcacagggg gcgtcgacgc cggaggtgtt gatctcgacg gccgttccgc
     2221 gcgcgagcat gcgcgggatg agcggctcga cgtaggggac cattccgccg gggggaaagt
     2281 ggccgaattt gccgggcagg tcgaggtggg cgatgatgtc gcagaagccc tcgtcgatca
     2341 tttcgccgac cagatgataa tagagctcgc agacgtcctc gatgcgggcg gtctcgaaga
     2401 agtgcgcgtg ggagggcttg ggcaggcccc agaagccgag gtgatggacc gagccgatga
     2461 ggtaatccca ggggaattgc tcgatggcgc ggcgcgcgat cgcgcggcgg ctggggacgt
     2521 aatccatctc gaggccgagg cgcagcgtga actggtcgct gttgaaccgg cggcggaggg
     2581 cctggacgtc ggcgacgtag tcgggcagtt cttccggggc catcgcgacg tgttcgccgc
     2641 gggcctgaat catccacggg ctgtgatcgg agaagccgaa ttccgagaaa ccgagggcga
     2701 tggcccgctg gacgtattgc tccatggcgc cgtgggcgtg ccggcagcgc caggtgtggc
     2761 tgtggtagtc aatcagcatg gggggatcct ttcagaaggg agagaaggga cataaaggat
     2821 gcaaggggcg caagggacgc aagagctttg agagcttgtt ttcctggtct cacggtgagg
     2881 ccggaggcgg cgtgagaacg ggacggcgct cctcgcgcgg ccagcggtcg aggagttcct
     2941 tgagcgggcg ccagacgtcc tcgaggggga tcagcgcgac catgtggttc tcgggcaggc
     3001 cgcggcgctc gtcgggcgtc aggtcgaatg tgcaggcgcg gatcgtccgg tggatcgggc
     3061 gctcccagta gtcggcccag cggcgctcgt ccatcgggcc atagagcgtg atgctgggga
     3121 tgtcgagggc gtgggccagg tgttgcgggc cgccgtcgac ggtcaccagc ccatggcagc
     3181 gctcgagcca ggcgaccagc tcgggcagcg gcgcggcgcg cagcaggagc gtgcgttcgc
     3241 cggcctgctc ggccgcgcgc tccagatcgc gcagcggggc gaacgcattg atgacgacgc
     3301 gggcgtcggt ctcggcgagg agccgcgcca gaagcgccgc ccagcgctcc tcggtccatt
     3361 ccttctgagc gtgcgcggcg gcggggttga gcatgacgag cggcccgggg cgcgccatca
     3421 ggggatggcg ggcgacgcgt tcggcggcgt cggcgggggg gcggatcagc gccggcgacg
     3481 gatcgccggc caagcccgcg acgcgcatca ggtgggccag gttatcgatc tcgtggagtt
     3541 cgagatgctt gtggacacgg acgccgaggc gctcccgatg gaggcggcgg cgcaggcctt
     3601 gatcgcggtc gaaggcgaag atcgggcgcg ggggatggcg gatcgacgcc agcgcgaccc
     3661 cccagggacc gtggaggtag ggcgcgacga tggcgtcggg atcgatgcgc tcgagccagc
     3721 ggagcagcca gtcgcgcgcc tcgcggcttt cgggcgggag gctggggagc agggtcaggt
     3781 tgagccgggg caattcgccg agggccgtcc gggcggcggg gggggcgagg acgtagacgc
     3841 cgaggcgcgg gttctcgcgc atgatgccgc gcaggacgca gtgcagcagg acgagatcgc
     3901 ccagataccc gagcgcgatg accgccacgc gttcgatcac cagggcgcgc ggccggcgcg
     3961 gccgggagac gatccagggg cggatgagcg cgtggacggc ggtgcggccg cgatcggcga
     4021 ggtagcgcgg cccggtgcgc gccagccgcg cgaggaccgc cgcccgggcg atcaggcgcg
     4081 gccggcgccc gaggcggctg gtgagcgcga cgagggcggc cgcgtgccgg cggcgcatca
     4141 gttgatgata atgccaggcg gccggcaggt tgtccggctc gcgaaacggc gcggcgaacg
     4201 cccggaggtg gtgatcccac caggcatagg cgaggtgatc caggtcgtca ggggttccct
     4261 cgcgctcggc ccaggcggcg ccgtcgaggc gggcgcgctc gcgcaggtag ggcagcgcca
     4321 ggcgtgcggg gtcgatggca tggccgaccc agagttgggg gtcgatgcgc gtccggcagc
     4381 cggcgcggcg cagcgtggcc caccactggg cgtcctcgcg gccggcgacg taatgttcgg
     4441 cgaccttgcc gcccagccgg ccgccgattt cctgaaacgg gaattcccgc gcgaccgcgg
     4501 cgtggaccca gaggttggcc gtcatcgggt agtgcagccg gccggcgtcg ggaccaacca
     4561 tgccggggac ggagaggccg accatccagg ccatgtcggg gtgccaccag ccgggcaggc
     4621 ggcggatgcc ccagggtcgg gcgatgccgc cgacggcgtc ggccgtggtc aggtcggcct
     4681 tgccgcgggc gacccagtcg ggggcggcca tgcagtcgtc gtcgaggaag gcgatcagat
     4741 cgccgcgggc ttcgcgcacg ccgcggttgc ggatttcggc ccagctgccg ccgtcgtcgt
     4801 gggcgaggaa gcgaaaccgg gggtgctgcc agtgggcgta atgcgcgcgc gtcgcatcgg
     4861 tggagccggt atcgatgacg agcatttcgt cacccggctc gagctggccg aggacgcgct
     4921 ggagacaatc ggccagccgg tggaagcggt tgcgggtgag cgaaatgacc gtcagcgtgg
     4981 gcatgccgaa aagctcagcg aagagtttcc cgccactgaa gtggcgggct gaagtctacc
     5041 gtccctgacg ggactggccg gggggcgccg ggggagtaat ccgccgctcc cctaccccta
     5101 ctcctccacg ccgagcgcgc gcaggtgctt cgttcgggcc tggtcgatgc cccagtagcg
     5161 gaaatgccac ggttcgggct gatagccggt ttccgcctcg ttgtgctcgg tgtagctctg
     5221 ggcgaagccg aactcccagg catgttcgcg cagccagcgt ccggcgggcg actcgccgaa
     5281 actgacgcgc agcagcgtct cttcgttccc gtcggtcagg tcgaccgcgg ttcccagttg
     5341 atgctcgctg tggccaggct tggccacggt cttctggtcg cgaccggccg ccttgacctt
     5401 gcgttcgtag agggtctgct gcacgctcca cggccggtag ccggagacga catacagggt
     5461 gtggccgtcg gctttagcgg cggcgagcat cgcggcgagc gcctcggccg cctcggcgcg
     5521 cagcttgtaa tcgcggtcct gctcgtagcg cgggccgacc gagaccaggt cgtcgggggc
     5581 gtaattgggc ggaaggccgg ccgtgcgatc gacgcgcgcc tgaccgatcg cgcgcagcga
     5641 cgactcatcg aaatcgaccg ggggaaggga gaggcaggcc tcgggcagcc agccggccgg
     5701 ggcatcctgg ccgacctgga accaggtttc gccgtgggcc gcctcgagcg ggcagtcggc
     5761 gaaaacggtc aactccgtgc cggccttcca gttcatcggg ggcccgttgg gttcgccggg
     5821 ccccggttcg agccagagcg tcggctggcc gatgagcagc aaccggccat gctcggcggc
     5881 gccggccggg gcgggcgcgc aaccggcggc gaggatcaat ccgaacatca ggattcgcgt
     5941 catgcattcg ctcctgggag tgctcgatca ggcgcgggcg aagatcgcgc ggcgcgccgt
     6001 gagggaaccg gcgtgttgac cgggctgcac ggtcggggtc gcgtcatggc cagcgttcgg
     6061 cgcgcaggat ggcgcgctcg ccggtgggca gcgccgcgag caacgcgccc cagccggccg
     6121 gcgcaatcgc cggcagcggc ggaacttgca ggtcgatcag cggcgcgagg acgaagtcgc
     6181 gccgggcgat ccacggatgc ggcagcgtca ggcccggcgc gtcgcggcgg accgacccga
     6241 agagcaggag gtcgaggtcg aggcg
//
LOCUS       DAOPCO010000238         6236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11030_length_6236_cov_5.237017, whole genome shotgun sequence.
ACCESSION   DAOPCO010000238 DAOPCO010000000
VERSION     DAOPCO010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11030_length_6236_cov_5.237017"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1173)
                     /locus_tag="PLS90_13720"
     CDS             complement(184..1173)
                     /locus_tag="PLS90_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPK03502.1"
                     /translation="MVMPEALAHIMRTASGYTHAIPLLTACQTRIFAALGRERRSAGA
                     LARELGMDERALETVLLALVADGFLTGDEAGFALAPGYGEFLLPDGKRTRASILSHHA
                     HLVPRWARLPEVLRSGEPVVATQDRTPDELRAFICGMANLSRESSLEAAEKLNVGQYH
                     RMLDLGGGPGTAAIVFARANPQLTAVVFDLPDPIEIAREEIAAAGLEDRIDTMAGDFM
                     RDDIGADYDLVYIANIIHMLGPEETEALLRKARAALVPGGTVAIKDFYLDDSRAAPNF
                     AAFFSINMLVNTPRGKSYTRTEVEQLLTRLGFQDLALLSVAEESAILTARKGS"
     gene            complement(1215..1760)
                     /gene="rimM"
                     /locus_tag="PLS90_13725"
     CDS             complement(1215..1760)
                     /gene="rimM"
                     /locus_tag="PLS90_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPK03503.1"
                     /translation="MSPIQPADLLAIAHIARPHGVRGELSAIALAPPVLAPESLLLGR
                     TLIGRDPRGRLSDYTAIGFRPHQGRWLIQLEGVTDRTQADALRGHDLCLRRRDLPDLP
                     EGWYWESDLEQCRVVDRRLGEIGRVEGLDTSGVQPYLTLIRPDGRRAMIPWRSALVPA
                     VDLEAGVIETDLPGDFPGISE"
     gene            2139..>6236
                     /locus_tag="PLS90_13730"
     CDS             2139..>6236
                     /locus_tag="PLS90_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK03504.1"
                     /translation="MLRKLTLFLTVVLLTLAGCERAPRDGAANDATVVQARVRPAPME
                     GGFVTLAEDYATTGLTVRLRWPSHLGLPRDMPVLAFVLPAGVKPAAAWRDVHYGGAPW
                     PPADELTTGSLAARYPGSPATPEFVGYLRRWPIYSLQVTADIFAPLQDGSDGQTGAPA
                     PADDLELTLSLSWRAPDAPTQEPSAADPAAKDAPSNASWRDLAARLVANPEGLSRYAV
                     ASPPPPEGERHELVDPREHAPSATRWSKLLVREEGLIHLPMEELIKAGVPSAEAVPSA
                     VRVFSRGRPVPLLVAPGPAVPSGWPAGVYFWATGGKTPYSRERAYWVTTDDSVAAALL
                     ARAPADAEPTTAALATIPRKYTYEQDNSLEMSHGSFLAVEAMEWIDAPLEPEQPLQLR
                     VELPHFAGGGALSGLLKFFLDTDRTLSQVRAEVEINRNPVATLKFYGARDATKQIDLP
                     ATAFADGAATLTITLYDERSPTEREEAPVLVWFDHLAITYPSQPRLLDGRLTIPGDAL
                     TSVATRAPILDAAGNPAPAAIALRIVGGGEAESYLPVQRDRDGTTYVTWVRPRTMGAM
                     PPEERIEIVTPEAARPVTNLRPLTFPDLRTPDEPLDLLIVTHAEFEEAARRLAEFHDG
                     RGWRTRVIDVEGVYDLFSHGTLDPGAIRGLLGHLLRAWGARAPGHVLLIGDCNSDYLD
                     VARKGVRNWIPTYTFENGAERWASDFWFTAVAGSDDLPDFMLGRLSVANRDDARRVVD
                     KIIQYGRRAMPGPWRARLGYVADNGEFTEVIDSLRREETPRQFTAERVYLDELPFEDN
                     WYLPQTMVERKRMKVSRAATDRILALFRGGVSYLTYYGHGSPNIWADERIWFGGDSPN
                     SDNLHLADSGWMTFVANMTCNSGAIDYPVPPWNICITEDMMRVENGGAIACYVPSGPG
                     ITSIHRRMSQALRRVLLREGVRQFGEIVTLAKARYALNRDPRELLYMYLLLGDPTLDL
                     QLVDELRELQLSRNVVAPGTNLTVELEGLEPREGRWTGELTSADGASLWTADEATYRG
                     GALTLRLDIPSTAPEGAAELRLYAWSPAGGAEMAAAGTIEIEYPELALDRLRVDPSAA
                     PPAAIATVRNPAKINSTGRLELLAEDGATTRVVLTRELSLAPGANLEVSAALPASPDR
                     PLIARAILHAARPHADPGQPLQFEQQVLASVPAGWTGWLPTLAAWSFDRPGLPSELHV
                     VAATREEPPPRLLARVLTAAGDTVGTQTLGYGAAAAGGMDTASTAEAALAEATFTLSR
                     DLVERLAEGALWLGRLTTETLTLEEVDRVPLALIPRRAADVRIVPGSARVRPPNPTEG
                     ETVFVDLLVENVGNIPSAPMMVSLLDKPPDAGGQPLPNQIEYNRDR"
BASE COUNT          945 a         2126 c         2182 g          983 t
ORIGIN      
        1 ccatgggcga acgctccgga gcggttatcc ggcgtcgcgt acctctaccg tctcatgcgg
       61 agtgcggcct gcgattggct ggattacccc taggcgggag agctggcagg cgccgaggga
      121 tcttcggggg gcagactttc cattgtgtgc cgtcactcgt ccctttcggc tgtcgtgctg
      181 gggtcaggaa ccttttcgcg cggtgaggat ggcgctttcc tccgcgacgg acagcagcgc
      241 gaggtcctgg aagcccagcc gcgtgaggag ttgctcgacc tcggtccggg tatacgactt
      301 gccgcggggc gtgttgacga gcatgttgat gctgaaaaac gcggcgaaat tgggcgcggc
      361 gcgcgaatca tcgagataga aatccttgat cgcgaccgtc ccgccgggga cgagcgccgc
      421 ccgcgccttg cgcagcagcg cctcggtctc ctccggcccc aacatgtgga taatgttggc
      481 gatatagacc aggtcgtagt cggcgccgat gtcgtcgcgc atgaaatcgc cggccatcgt
      541 gtcaatacgg tcctccagcc cggcggcggc gatttcctcc cgcgcgatct cgatcggatc
      601 gggcaggtca aacacgaccg cggtgagttg cggattcgcc cgcgcgaaca cgatcgccgc
      661 cgtgcccggt ccgccgccca ggtcgagcat gcggtgatac tggccgacgt tcagcttctc
      721 ggcggcttcc aggctcgact cgcgcgagag gttggccatg ccgcagatga acgcgcgcag
      781 ttcgtcgggc gtgcggtcct gggtcgcgac gaccggctcg ccgctgcgca gcacctcggg
      841 caaccgggcc cagcgcggaa ccagatgggc gtggtggctc aggatgctgg cgcgcgtacg
      901 cttgccgtcg ggcagcagaa attctccata gcccggtgcc agggcgaagc ccgcctcgtc
      961 gcccgtcaga aagccgtcgg cgacgagcgc cagcaggacc gtttccagcg cccgctcgtc
     1021 catgcccagc tcacgcgcca gcgccccggc cgaccgccgt tcacgcccga gcgcggcgaa
     1081 aatcctggtc tggcaggccg tgagcagggg gatcgcatgc gtgtagccgg acgcggttcg
     1141 catgatgtgc gcgagagctt cgggcatgac catgatacaa gcctttcctt acaacagatt
     1201 gcgcgagatg cgggctactc gctgattccg ggaaagtccc ccggcagatc ggtctcgatc
     1261 acgccggcct ccaggtcgac cgcggggacc agcgccgagc gccatgggat catcgcgcgg
     1321 cggccgtcgg ggcggatcag tgtcagatag ggctggacac cgctcgtatc caacccttcg
     1381 acgcggccga tctcgcccag gcggcggtcg acgacgcggc actgttccag gtccgattcc
     1441 cagtaccacc cctcgggcag atcgggcaga tcgcggcggc gcaggcagag gtcgtgcccc
     1501 cggagcgcat cggcctgcgt gcggtcggtg acgccctcca actggatcag ccagcgcccc
     1561 tgatgcgggc gaaatccgat ggccgtgtag tcggaaagtc ggccgcgggg gtcgcggccg
     1621 atgagcgtcc ggcccaggag cagcgattcg ggcgcgagga ccggcggcgc caatgcgatc
     1681 gcgctcagtt cgccccgcac gccgtggggg cgggcgatgt gggcgatcgc aagcaggtcg
     1741 gcgggttgga tgggggacac gggctggggc ttcccgggcg agtcgtggag ctccgcgaag
     1801 cggcggcgtg gcttcagcaa ccattcttga gccggaaacc ggattgtcaa tgatccagca
     1861 agacggggtg cgacgctgat ctggtgcatt gcgatcgcta tcgggatcgg gatcgagtca
     1921 gcccccgtgc atcccgcgcg gcgtgaggca aacaaaactc cttgttcttt attgcttcct
     1981 tgctgttcct caggttccga atcggccgcg ccgtatggac aggcggcagc ggatgattta
     2041 cgatgatcgg ggctgacgag cgtcgtcacc ggccgccagc cctgaccgtc caaaccgggg
     2101 gatttccagc cctgccttcc gttggttccg tttctccgtt gctgcgcaag ctcacgctgt
     2161 tcctcacggt tgtcctcctg acgctcgcgg gatgcgagcg cgcgccgcgc gacggcgccg
     2221 cgaacgacgc gaccgtcgtc caggcgcgcg tgcggccggc gccgatggaa ggcggatttg
     2281 tcacgctcgc cgaagactac gccacgaccg gcctgaccgt gcgcctgcgc tggccgtcac
     2341 atctgggcct gccgcgcgac atgccggtgc tggcgtttgt gctgccggcc ggggtcaagc
     2401 cggcggccgc ctggcgcgac gtgcactatg gcggggcgcc ctggccgccc gccgacgagt
     2461 tgaccaccgg ctcgcttgcg gcgcgctatc cgggatcgcc ggcgactccc gaattcgtcg
     2521 gctatctgcg tcgctggccg atctactcgt tgcaggtgac ggcggatatt ttcgccccgc
     2581 tgcaggacgg gagcgacggg cagaccggcg cgcccgcgcc ggcggatgac ctcgagctga
     2641 cgctgtcgct gagctggcgc gcgcccgacg cccccacgca ggagccgtcc gccgccgacc
     2701 ccgccgcgaa ggatgcgccg tcgaacgcca gttggcggga tctcgccgcc cggctggttg
     2761 ccaacccgga agggctgtcg cgctacgcgg tggcgtcccc gccgcccccc gagggcgagc
     2821 gccacgaact ggttgacccc cgcgagcacg cgccgtccgc gacgcgatgg tccaaactgc
     2881 tcgtccgtga ggaagggctg atccatctgc cgatggagga actgatcaag gcgggcgtcc
     2941 ccagcgcgga agctgtgccg tcggcggtgc gggttttctc gcgcgggcgg ccggtaccgt
     3001 tgctcgtggc gccggggccg gcggttccct ccggttggcc ggccggcgtc tatttctggg
     3061 ccacgggtgg caagacgcct tactcgcgcg agcgcgccta ctgggtcacg accgacgaca
     3121 gcgtggcggc ggcgctgctg gcgcgcgcgc ccgccgacgc cgagccgacc acggccgcac
     3181 tcgccacgat cccgcgcaaa tacacctatg aacaggacaa ttcgctcgag atgagccacg
     3241 gcagtttcct ggccgtcgag gcgatggaat ggatcgacgc gccgctggag ccggagcagc
     3301 cgctgcagct gcgggtcgaa ctgccgcatt tcgccggcgg cggcgcgctg agcggcttgc
     3361 tgaagttttt cctcgatacc gaccgcacgc tgtcgcaggt gcgcgccgaa gtcgagatca
     3421 accgtaatcc ggtcgcgacg ctcaagttct acggcgcgcg cgacgcaacc aagcagatcg
     3481 atctgccggc caccgcgttc gccgacggcg cggctacgct gacgatcacg ctgtacgacg
     3541 aacgcagtcc gaccgagcgc gaagaggccc ccgtcctggt ctggttcgac cacctggcaa
     3601 tcacgtatcc cagccagccg cggctgctgg acggccggct gacgatcccc ggcgatgcgc
     3661 tgacctccgt cgcgacccgc gcgccgattc tggatgcggc gggcaacccc gcacccgcgg
     3721 cgatcgccct gcggatcgtc gggggcgggg aggcggagtc gtatctgccg gttcagcgcg
     3781 atcgcgacgg cacgacttac gtcacctggg tgcggccccg cacgatgggg gcgatgccgc
     3841 ccgaggaacg gatcgagatc gtcacgccgg aagcggcccg gcccgtcacc aacctgcggc
     3901 ccctgacatt tcctgacctg cggaccccgg atgagccgct cgacctgctg atcgtgaccc
     3961 acgcggaatt cgaggaagcg gcgcggcggc tggccgaatt ccatgacggc cggggctggc
     4021 gcacgcgcgt gatcgacgtg gaaggcgtct acgatctctt cagccacggc acgctggatc
     4081 ccggcgcgat ccgcggcctg ctgggccatc tgctgcgcgc gtggggcgcg cgcgcgccgg
     4141 gacacgtcct gctgattggc gactgcaaca gcgactatct ggacgtcgcc cgcaagggcg
     4201 tgcgcaactg gatcccgacc tacacgttcg aaaatggcgc ggaacgctgg gcctcggact
     4261 tctggttcac ggccgtggcg ggctcggatg atctgcccga cttcatgctg ggccggctgt
     4321 cggtcgctaa tcgcgacgac gcccgccgcg tcgtcgacaa gattatccag tacggccggc
     4381 gcgcgatgcc ggggccgtgg cgcgcccggc tgggttacgt cgccgacaac ggcgaattca
     4441 ccgaagtgat cgattcgctg cggcgcgagg aaaccccccg ccagttcacg gccgaacgcg
     4501 tttacctgga cgagttgccg ttcgaggaca actggtatct gccccagacg atggtggagc
     4561 gcaagcggat gaaggtcagc cgcgcggcga ctgaccggat tcttgccctg ttccgcggcg
     4621 gcgtctcgta cctgacatat tacgggcacg gatcgcccaa tatctgggct gatgagcgga
     4681 tctggttcgg cggcgattcg cccaacagcg acaacctgca tctggccgac tcgggctgga
     4741 tgacgttcgt cgccaacatg acctgcaact ccggcgcgat cgattacccc gtgcccccct
     4801 ggaacatctg catcaccgag gacatgatgc gcgtggagaa cggcggcgcg atcgcgtgct
     4861 acgtgccgtc ggggccgggg atcacgagca tccaccggcg gatgtcgcag gccctgcgcc
     4921 gcgtgctgct gcgcgagggc gtgcgccagt tcggcgagat cgtcacgctt gccaaggcgc
     4981 gctacgcgct caaccgcgat ccgcgcgagt tgctctacat gtatctgctg cttggcgacc
     5041 cgacgctgga cctgcaactg gtggacgaat tgagagagct gcagctgtcg cgcaacgtag
     5101 tcgcgccggg gacgaatctg accgtggagc tggaggggct tgagccgcgg gaggggcgct
     5161 ggacgggcga attgacctcg gccgacggcg cgtcgctctg gacggccgac gaggcgacgt
     5221 atcgcggcgg cgcgctgacg ttgcgcctgg acatcccgag caccgccccc gagggcgccg
     5281 ctgaattgcg cctctatgcc tggtcgccgg cgggcggcgc ggagatggcc gcggccggca
     5341 caatcgaaat cgaatatccc gaactggcgc tcgaccgtct gcgcgtcgac ccgagcgccg
     5401 cgccgccggc tgccatcgcc accgtccgca atccggcgaa aatcaactct accggccgcc
     5461 ttgagctgct ggcggaggat ggcgcgacaa cgcgggtggt gctgacccgc gagctgtcgc
     5521 tcgcgcccgg cgcgaacctg gaggtctcgg ccgcgctgcc ggcctcgcct gatcggccgc
     5581 tgatcgcgcg cgcgatcctg cacgccgcgc ggccgcatgc cgatccgggt caaccgctgc
     5641 aattcgaaca acaggtgctg gcgagcgtgc cggcgggctg gaccggctgg cttcccacgc
     5701 tggccgcctg gtcgttcgac cggccgggtc tgcccagcga gctgcacgtc gtggcggcga
     5761 cgcgcgagga acccccgcca cgcttgctcg cccgcgtgct gacggccgcg ggcgataccg
     5821 tgggaaccca gacactgggt tacggcgccg cggccgccgg aggcatggac accgccagca
     5881 ccgccgaggc cgcgctggcc gaggcgacgt tcaccctctc gcgcgacctg gtcgaacggt
     5941 tggccgaagg tgcgttgtgg ctcggacggc tcaccaccga gacattgacg cttgaggagg
     6001 tggaccgggt tcctttggca ctcatcccgc ggcgggcggc cgacgtgcgg atcgtcccgg
     6061 ggagcgcgcg cgtccgcccg cccaatccca ccgaggggga aacggtcttt gtcgatctgc
     6121 tcgtggaaaa cgtcggcaac atcccgtcgg cgccgatgat ggtctcgctg ctcgacaagc
     6181 cgcccgacgc ggggggccag ccgctgccga accagatcga atacaaccgc gaccgg
//
LOCUS       DAOPCO010000239         6195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11127_length_6195_cov_4.757818, whole genome shotgun sequence.
ACCESSION   DAOPCO010000239 DAOPCO010000000
VERSION     DAOPCO010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_6195_cov_4.757818"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLS90_13735"
     CDS             complement(<1..482)
                     /locus_tag="PLS90_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HPK03505.1"
                     /translation="MPELPEVETVARQLAPALTGRRVVAVQVFDPKIDPASLEPLIGR
                     TITAVRRIGKQVGIGFSQPGIPEAGPWLCIHLRMTGRLAWAPDCRQPPVAPLRVRFRL
                     DRGALLFHDTRRFGTLTLYPTLRDATPAGIDPTGRAFTPARLAAWLAQSASPIKVWLM
                     R"
     gene            645..1424
                     /locus_tag="PLS90_13740"
     CDS             645..1424
                     /locus_tag="PLS90_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03506.1"
                     /translation="MAESVVSAEQVSRRRAFRPFLIAAIALVVVFLAAAVGGGIYTSL
                     QYRELVDLGKETQADYARLDEAYPFEPPRGAAAAPSRWEAMLSVRARALAALSPAARV
                     QLAKLLELQSLKEVDPVYLARLLPLGGQLKAVVDAHLAALAEHEMSGEEYRWYLSQAV
                     YQVMKETREAAPAAAAHWRVLHVAEALSRTDEDSRNDLTGEEFLERIKREYGPLERAD
                     ASVTGPLRASDPVAAMLDLLAVASGWARMEQLTTQEKPAGS"
     gene            1444..3942
                     /locus_tag="PLS90_13745"
     CDS             1444..3942
                     /locus_tag="PLS90_13745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPK03507.1"
                     /translation="MSSATTTYPGRSRLRRKSRPRRRIRIRFRFRFWRALFALVAFFI
                     TLGGAIGAGLFIGYVHSLPPIEQLEHYDPPQVTRVYDRSGAKAIGEFKQENRQVTPIN
                     EIPRMLRNAFIAVEDRRFYEHWGVDLRGVIRALVRNIKAGRIVEGASTITIQLAGAIL
                     PEIERSERTIGRKVIEAFRAMQIERRYTKDQILEFYLNQIPFSYNAFGVRAAAATYFS
                     KDMADLTIAECATLAGMVKGQSVYNPISNVERSTERRNLVLAKMLENGFITEEEYEAA
                     VKEPMQVRRGFGVTSAYPYFLDGLRRDLINFYGESIVSLGQAGLRISSTIDPAIQEAA
                     IKALRDGLAGNEEWEGVEALWQSKKPERAEEEMKNWKGRIDPGDTYLMEITGVREDEL
                     DVRLRWYRGTIPIPDPPPFHNPEGKLDIGQLIDVRVETVDQERSTFSGQYAFSSPVQG
                     SIVVLDAHSGDVLALVGGVNFYNKAWNGQYNRAMMDGRQPGSCFKPFYYAAAFEKGLT
                     PADMIVDEPIEYYVYNTVYRPRNYEKYFAGNMTLIEALEHSRNIPTIRLYEALGPRTA
                     NEIVGRFDYVGRSRRWQIPNEYSTPLGTIDCTPLEMAAAYLAFVNWGVGVRPQFFRNA
                     VDANGRIQIPVERKEGPIISPIAAYQTLYLMRQVVRSGTGRRPIGEKFPSPPFPQIAG
                     KTGTTNDNRDAWFCGVTPDLVIVVQIGFDQYHPMGPGMTGGSVAGPVWAMTFREILKT
                     RPSWTLEFTAPPGIVYADICKNTGKRVGPACEWEGHAVYKQVPFKEGTVPEQDTNGYE
                     RVPLIQPVNSEYSYFSGQSPRVGRASPASNRSYD"
     assembly_gap    3997..4096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4121..4465)
                     /locus_tag="PLS90_13750"
     CDS             complement(4121..4465)
                     /locus_tag="PLS90_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPK03508.1"
                     /translation="MDKLARKVEKLNLARASRHIFLCTDDDCCQSRAARHAWKFLKQR
                     LKELKLDGQGGVLRTRARCFDVCTGGPIAVVYPDAVWYHGCTPDVLERIIQEHLIGGR
                     VVEEFRLPGSVD"
     gene            4611..>6195
                     /locus_tag="PLS90_13755"
     CDS             4611..>6195
                     /locus_tag="PLS90_13755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HPK03509.1"
                     /translation="MFAWSRFNPCQSTPAFIAAMVLIAALGLALRLPGLGRRPLHVDE
                     AVQACKAGILYDSGEYRYNPREFHGPTLYYATLPVLWLSGAERAEEAGARTFRLVPVV
                     FGVGLILLGLLLRRGTGRPAALAAALLTAVSPAFTYYSRYYIQEMVLVFLTFLLILAG
                     WHGGRGRGWFWAGVAGVTVGLMHATKETWVLAAGAMALALGGAAWWRRALGEPARRGR
                     RWRRPGPVALAAGLALGVAWLLYSGWPRDGDGLLNSFRAFGYYAARAAQGEGSAALHR
                     HPWHTYPRLLSWAGLGPGPWWSEALVLGLGLAGMTAVVLGHTGAGARCLAGIDRPLAR
                     FLALYTILLTVIYCAIPYKTPWNLLSFYHGFVLMAGIGAVAVIRLPRGRWARGVVGAV
                     LLGLVAQLGWQAWRGAHRFEVDARNPYVYAHTSTDALRLVERLNDLAAVDPDGRAMLI
                     RIESAEYWPLPFYLRGFARVGYYEGEELERQTQATSAPVVITRPDREAQVEGRLRGDY
                     QKEYYGLRPEVLLTVYIRRD"
BASE COUNT          981 a         1685 c         2416 g         1013 t
ORIGIN      
        1 cgcatcagcc agaccttgat gggtgaagcg gactgggcga gccatgcggc gaggcgggcg
       61 ggggtgaacg cgcggccggt cggatcgatg ccggcgggcg tggcgtcgcg gagcgtgggg
      121 tagagggtga gggtgccgaa ccggcgggta tcgtggaaga ggagggcgcc gcggtcgagc
      181 cggaagcgga cgcggagggg cgcgaccggc ggctggcggc agtcgggcgc ccaggcgagc
      241 cggccggtca tgcgcaggtg aatgcagagc caggggccgg cttccgggat gccgggttga
      301 gagaagccga tgccgacctg tttgccgatg cggcgaaccg cggtgatggt ccggccaatg
      361 aggggctcca gagaagcggg atcgattttg ggatcgaaga cctggacggc gacgacgcgg
      421 cggccggtca gcgcgggggc gagttggcgc gcgaccgttt cgacttcagg cagttcaggc
      481 ataaggggaa agacctttca tggagaggaa gggagagcaa ggccctggtt gggtcaggga
      541 attcattttt gccggaaggg gagggaatgc gctagaatcg tgcgtcgctt gcccggcgga
      601 agccggcggc gggcggccag ttcgctgcgc agcagggaga agccatggcc gagtcggtgg
      661 tgagcgccga gcaagtcagt cgccggcggg cgtttcgccc gttcctgatc gcggcgatcg
      721 cgctggtggt cgtgtttctg gcagccgccg tggggggcgg gatctacacg tcgctgcagt
      781 accgcgaact ggtggacctg ggcaaggaga cgcaggccga ttacgcgcgc ctggacgagg
      841 cgtatccgtt cgagccgccg aggggcgcgg cggcggcgcc gtcgcgctgg gaggcgatgc
      901 tgtcggtgcg ggcgcgggcg ctggcggcgc tctcgccggc ggcgcgggtg caactggcca
      961 agctgctgga gctgcagtcg ctcaaggaag tcgatccggt gtatctggcc cggctgctgc
     1021 cgctgggcgg gcagctcaag gcggtggtcg acgcgcatct ggcggcgctg gccgaacatg
     1081 aaatgagcgg cgaggagtat cgctggtatc tgagccaggc ggtttaccag gtgatgaagg
     1141 agacgcggga ggcggcgccc gcggcggcgg cgcactggcg ggtgttgcac gtggcggagg
     1201 ccctgagccg gaccgatgag gacagccgca acgacctgac gggggaggaa tttttagaac
     1261 ggatcaagcg ggaatatggc ccgctcgaac gagccgacgc cagcgtgacc gggccgttgc
     1321 gcgccagcga tccggtggcg gcgatgctcg acctgctggc ggtggcttcg ggttgggccc
     1381 ggatggagca attgacgacg caggagaagc ccgccggctc gtaagccggg cggaatccgt
     1441 gcgatgagca gcgcgacgac gacctacccc ggccggagcc gactccggcg caagagccgg
     1501 ccgcgccgcc ggatacggat ccgctttcgt ttccggttct ggcgggcgct gttcgcgctg
     1561 gtggcgttct tcatcacgct ggggggcgcg atcggggcgg ggctgttcat cgggtatgtt
     1621 catagtctgc cgccgatcga gcagctcgag cattatgatc cgccgcaggt gacgcgggtg
     1681 tatgaccgga gcggggcgaa ggcgatcggg gagttcaagc aggaaaaccg tcaggtcacg
     1741 ccgattaatg aaattccgcg gatgctgcgc aacgcgttca tcgcggtgga ggaccgccgg
     1801 ttttacgaac actggggggt ggatctgcgc ggcgtgatcc gcgcgctggt gcgcaacatc
     1861 aaggcgggcc ggatcgtgga gggggcgagc acgatcacga tccagttggc gggggcgatc
     1921 ctgccggaga ttgagcggtc cgaacggacg atcgggcgca aggtgattga agccttccgg
     1981 gcgatgcaga tcgagcggcg gtatacgaag gatcagatcc tcgaatttta cctgaaccag
     2041 attccgttca gctacaacgc gttcggcgtg cgggccgcgg cggcgaccta tttttcaaag
     2101 gacatggcgg atctgacgat cgccgagtgc gcgacgctgg cggggatggt caagggccag
     2161 agcgtctaca atccgatcag caacgtggag cgatcgaccg aacggcgcaa tctggtgctg
     2221 gcgaagatgc tggagaacgg gttcatcacc gaggaagagt atgaagcggc ggtgaaggag
     2281 ccgatgcagg tgcggcgcgg gttcggcgtg acgagcgcct atccgtactt tctggatgga
     2341 ctgcgccgcg acctgatcaa cttttacggc gagagcatcg tctcgctggg ccaggcgggg
     2401 ctgcgcattt cgtcgacgat cgacccggcg atccaggagg cggcgatcaa ggcgctgcgc
     2461 gatgggctgg ccggcaatga ggaatgggaa ggggtcgagg cgttgtggca gtcgaagaag
     2521 ccggaacgcg ccgaggagga gatgaagaac tggaaggggc ggattgaccc gggcgacacg
     2581 tatctgatgg agatcaccgg cgtgcgcgag gatgagctgg acgtgcggct gcgctggtat
     2641 cgcggaacga ttccgatccc cgatccgccg ccgtttcaca atccggaagg aaagctcgac
     2701 atcggccagt tgatcgacgt gcgggtggag acggtcgatc aggaacggag cacgttcagc
     2761 gggcagtatg cgttcagcag cccggtgcag gggagcatcg tcgtgctgga cgcgcacagc
     2821 ggggacgtgc tggcgctggt gggaggagtc aacttctaca acaaggcgtg gaatggccag
     2881 tacaaccggg cgatgatgga cgggcggcag ccggggtcgt gtttcaagcc gttctattac
     2941 gcggcggcgt tcgagaaggg gctgacgccg gcggacatga tcgtcgatga accgatagag
     3001 tattatgttt acaatacggt ctatcggccg cggaattatg agaagtattt cgccggcaac
     3061 atgacgctga tcgaggcgct ggagcattcg cgcaacatcc cgacgatccg gctttacgag
     3121 gcgctgggtc cgcgcacggc gaatgaaatc gtgggccgat tcgactacgt cgggcggagc
     3181 cggcgctggc agattccgaa cgagtattcg acgccgctgg ggacgatcga ctgcacgccg
     3241 ctggagatgg cggcggcgta tctggcgttc gtcaactggg gggtgggggt gcggccgcag
     3301 ttcttcagga acgcggtcga tgcgaatggg cggatccaga tcccggtcga gcgcaaggaa
     3361 gggccgatca tcagcccgat cgcggcgtat cagacgctgt atctgatgcg ccaggtggtt
     3421 cgcagcggga cgggccggcg gccgatcggc gagaaattcc cgagcccccc cttcccgcag
     3481 atcgccggca agacggggac gaccaacgac aaccgcgacg cgtggttctg cggggtgacg
     3541 cccgacctgg tgatcgtggt gcagatcgga ttcgaccagt atcatccgat gggtccgggg
     3601 atgacgggcg gcagcgtcgc cggtccggtc tgggccatga cgttccggga gatcctgaag
     3661 acgcggccga gctggacgct ggagttcacc gcgccgccgg ggattgttta tgccgatatc
     3721 tgcaagaaca ccggcaagcg ggtcgggccg gcctgcgagt gggaaggtca cgcggtctac
     3781 aagcaggtcc cgttcaagga agggaccgtt ccggaacagg acacgaacgg ctacgagcgc
     3841 gtgccgctga ttcagccggt caattcggaa tacagttatt tctccgggca gtcgccgcgg
     3901 gtcgggcgcg ccagccccgc cagcaaccgg tcctacgatt aggatgtttg tcgaagagtc
     3961 gctcgctgcg gggacggggg taattcgtaa gagttgnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnacga gagacgattc ttgaatgagg ctagtcgact gaaccgggca
     4141 ggcggaattc ctcgacgacg cgaccgccga tgaggtgttc ctggatgatg cgttcgagga
     4201 cgtcgggcgt gcagccgtgg taccagacgg cgtcgggata gacgacggcg atcggtccgc
     4261 cggtgcagac gtcgaagcac ctggcgcggg tgcggaggac gccgccctgg ccgtcgagct
     4321 tgagttcctt gaggcgctgc ttgaggaact tccaggcatg gcgggcggcg cgggactggc
     4381 agcagtcgtc gtcggtgcag agaaagatgt ggcgcgaggc ccgcgcgagg ttgagctttt
     4441 cgacttttct ggccagcttg tccattgccc accgcatccg gggagagggg ccgcgagggg
     4501 cgaccccgcc ggatcatcca gagcaaaaat tcggcccgcc gcgccgccgc ttgcagtgac
     4561 ggcggcttcg gttagactga agccgcatct cgtccggtcc cggaatgccc atgtttgcct
     4621 ggtcgcggtt caacccctgt caatccacgc cggcgttcat cgcggcgatg gtgctgattg
     4681 cggcgctggg gctggcgttg cggctgccgg ggctggggcg ccgaccgctg catgtggatg
     4741 aggcggtgca ggcctgcaag gcgggaatac tgtacgacag cggcgagtat cgttacaacc
     4801 cccgcgaatt tcacgggccg acgctgtatt acgcgacgct gccggtgctg tggctgagcg
     4861 gcgcggaacg ggcggaggaa gcgggggcgc gcacgttccg gctcgtgccg gtggtgtttg
     4921 gggtcggcct gatcctgctg gggctgctgc tgcggcgggg gacggggcgg ccggcggcgc
     4981 ttgcggccgc actgctgacc gccgtctcgc cggcgtttac gtattacagc cgctactaca
     5041 tccaggagat ggtgctggtt tttctgacgt tcctgctgat tctggcgggg tggcatgggg
     5101 ggcgcgggcg cgggtggttc tgggcggggg tggccggggt gaccgtcggg ctgatgcacg
     5161 caaccaagga gacgtgggtg cttgcggcgg gggcgatggc gctggcgctg gggggggccg
     5221 cgtggtggag gcgggcgctg ggcgagccgg cgcgccgggg gcggcgctgg cggcggcccg
     5281 gcccggttgc gctggcggcg gggctcgccc tcggcgtggc ctggctcctg tattcgggct
     5341 ggccgcgcga cggggacggc ttgctgaatt cgttccgcgc gttcgggtat tacgcggcgc
     5401 gcgccgccca gggggaaggt tccgccgccc tgcaccgcca cccgtggcat acctacccgc
     5461 ggctgctgag ctgggcgggg ctgggtccgg gtccgtggtg gagcgaggcg ctggtgctcg
     5521 ggctgggact ggccgggatg acggcggtgg tgctgggcca cacgggggcg ggcgcccgct
     5581 gcctggcggg gatcgaccgg ccgctggcgc gcttcctggc gctgtatacg attctgctga
     5641 cggtgattta ctgcgccatt ccctacaaga cgccgtggaa cctgctctcg ttctatcacg
     5701 ggttcgtgct gatggcgggg atcggcgccg tggcggtgat ccgtctgccg cgggggcgct
     5761 gggcgcgggg cgtggtgggg gccgtgctgc tgggcctggt cgcgcagctc ggctggcagg
     5821 cgtggcgggg cgcgcaccgg ttcgaggtcg atgcgcggaa tccgtacgtc tatgcccata
     5881 cttcgaccga cgcattgcgg ctggtcgagc ggctgaacga cctggcggcg gtggacccgg
     5941 acggcagggc gatgctgatc cggattgagt cggcggagta ctggccgctg ccgttttatc
     6001 tgcgggggtt cgcgcgcgtg ggatactacg agggggagga gctcgagcgg cagacgcagg
     6061 cgacgtcggc gccggtggtc atcacgcgac ccgaccggga ggcgcaggtc gagggtcggc
     6121 tgcggggcga ctatcagaag gaatactatg gtctgcgccc ggaagtgctg ttgacggtct
     6181 acattcgccg ggatc
//
LOCUS       DAOPCO010000240         6185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11148_length_6185_cov_5.741762, whole genome shotgun sequence.
ACCESSION   DAOPCO010000240 DAOPCO010000000
VERSION     DAOPCO010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11148_length_6185_cov_5.741762"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(469..544)
                     /locus_tag="PLS90_13760"
     tRNA            complement(469..544)
                     /locus_tag="PLS90_13760"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(509..511),aa:Asn,seq:gtt)
     gene            complement(908..2776)
                     /locus_tag="PLS90_13765"
     CDS             complement(908..2776)
                     /locus_tag="PLS90_13765"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPK03510.1"
                     /translation="MAHGKKKPGRADQAAPQAGAGAALPHEPTPPPRAGAGRAVAGAV
                     AAAAGLIYLTTAAPGAWWGDGQELACAAWTLGIPHPTGYPLYTVLGHLWLKGLAHLDP
                     GRALTLFNVVCQAVAAGLLFRLFQQTLVRDPANPADQRARAGVMAALPAAGAALIVAL
                     SRTVWEQATFAEVYALNYVLAAGLLLAVWHDGAPRGPRHAAAAGVMLGLMGLNHYSCV
                     AFAPLAALVLLRWGRGAGQPWRIPLAGVAGFVIPLAGYLYLPLRARANPPLNWGDPSS
                     LRNLIWVLSGGQFRELRMGADQGSAAGGLLGWIGWWGYQLAPGAYATPGVCLVVGLIV
                     LAVAIGGLILLARARWELGAGLLATLALTAGFGMFYQIPDIDAYFLPAAPAAALGWIV
                     SVRWALAALGRRWGELPLGPLSRAVPVLVAGAMLLWHYPRLNKSWDDAPAVWGEAVMN
                     ALPDGAVVLTGGDADTYCLWYQQMVLGRRKDVSIVGSNFIFSGWYARYFEAEGRPRIP
                     VAIRDQAPGHKLTFDVALFEGTILPNLAAGRPLFATFADPLFAEYLRAEPVETLLPAS
                     YYEQSLYPAPFLPAPVLYRLHDNAPLRALSGAALAREFERWYRRPPGSAPVPRPPR"
     gene            complement(2839..3846)
                     /locus_tag="PLS90_13770"
     CDS             complement(2839..3846)
                     /locus_tag="PLS90_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03511.1"
                     /translation="MFRRFLKAAGRAALLGGLLAAAGCAWRQPRLHALRVDQVALPNR
                     IIEPAVWGRAKEIEVTARTPAGVRLPVRLRAIHDGRALGLLISWPDRSEGDTPPVWVW
                     DEQRHEYRWVSRPNDEVALLWPMSPDPTFAAVPEQPEGYDLWRWRGGWTNLSGHADDA
                     RLLLRQHPRGTDPATVEGELFPVPGTDRWIERQVIPDTGQPGTRPTTRPLLREEERMP
                     GTAADFASGSQADVEALALYAKVRDFQPSAYFRYGRRRTPGYWFSDEIDHAEDGFWFV
                     EFHRLLVTPHDEEDAQLRGDGPHRFAVWVVDEGQGPDPFRTGPIELRLQPSKQTRDDR
                     D"
     gene            complement(4126..5163)
                     /locus_tag="PLS90_13775"
     CDS             complement(4126..5163)
                     /locus_tag="PLS90_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HPK03512.1"
                     /translation="MVSGLQISVDFCREVVMPWLRHELGDDFDRVAVAVIGTGSDVLG
                     LDDDLSRDHHWSPRANIMLCRADADRLLPRLVAALENRPPDNFRDYSIRYDRTGMLGL
                     CANVVEDFFARFLGTDQLPRTDADWLALCEVDLSHVTSGAIVHDGPGELTRRREHLAY
                     YPDAIWKKRIADWCMYITGREAPYNYNRAVKRADELTGLIYLGQYFKRVMEMCYMLNR
                     RYGPYTKWLNRTFRRLPKYVDRIAPGLDAVLAETDGRRRVFQMVEVNRIIAEALHDLG
                     LTAPPRPRPFDESLTDLTLYDSAVEIYRTLPPGLLAPSFNQIELWEKFAREVLFDPED
                     YFVKLQEKTGV"
     gene            complement(5301..6095)
                     /locus_tag="PLS90_13780"
     CDS             complement(5301..6095)
                     /locus_tag="PLS90_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK03513.1"
                     /translation="MNLNEMAAFYREHGYLLAEGLLTPAEVEAMREEADATIERMSRH
                     QQEANNLWKGNWLKDEERAQQRLNAVHDMQFQAAIFGKLLFNDKLLDIAEALIGPNVQ
                     LHHTKMLAKPPEKGGAFPMHQDYPYFPHQNHTMFAVSIYLDDADEENGCIRVMPGSHK
                     LGPLECDPGGFYLPPEKYPIEEGLPVKAKAGDGVIFNYLTIHGSSPNRSGRPRRNILI
                     QMRDPSDPPTRDIHASRGQGLMLRGINPVPVPAAWAAERKAAAGVS"
BASE COUNT         1082 a         2093 c         2045 g          965 t
ORIGIN      
        1 ggagttcagg ctctgtaaga accttgcaaa agattccaat tccagtgttc ttgttcatgc
       61 cctctggcgc taaacagccg tctcgcaaag gttggtcaag ggcgggcgga aagctgaccg
      121 cccggtaaac acctcggcac atcccgatcc ttggattagc ttcgcttgcg ccgctgggct
      181 gtgtgggttg cttccggggt gggcaaaagg ttgtgatctg ttaaccctgt atggagcgtt
      241 aacgaggatt tcccatgttt cgagagggtt cggagagaga aacgggccga accatgatgg
      301 ttggctctgg tggaacggaa cgcgcgtttg aaacagaggg aaccgagaga gaggtgggga
      361 atggtccaaa ccctttagaa tcagggcgaa tcggcggcca tcgccgcagg cgtggcccga
      421 tggtctcctg ttaacgaaaa agccccctcg aaagggggct tgaagagttg gctccggcgg
      481 taggactcga acctacaacc aacggattaa cagtccgctg ctctgccgat tgagctacgc
      541 cggagcaagc caaacgcggc cctatcttga gcgaggaggg tgatgcttgt caagcatgtc
      601 ggcggtgatt tgtcagggcg agagtccctg cggacgagaa cgacttgcgc acgcgccggc
      661 ggatcacaac aagggatgaa gatggggggt gaacggccga tccgaacggt ccagaccttg
      721 ccggatacat cagcagggca gccccgtcag ggacggtaga cttcagcccg ccacttcagc
      781 aggaaccggg atacccatcg caacagagac ccgtgggggc ggcagaacca acgtgagcga
      841 tctcaaggtt aggggacgaa atctcgagtg cgggacagag gcaatcacgt agacgaggga
      901 cgaatgctca acgtggaggg cgcgggacgg gagctgagcc cggcgggcgc cgataccacc
      961 gctcgaactc ccgcgcgagc gcagcgccgc tcaacgcgcg cagcggcgcg ttgtcgtgta
     1021 gccggtagag tacgggcgcg ggcagaaacg gcgcggggta cagcgactgc tcgtaataac
     1081 tggccggcag cagcgtctcg accggctcgg cccgcaggta ttcggcgaac agcgggtcgg
     1141 cgaacgtggc gaacagcggc cggccggccg ccaggttggg cagaatcgtc ccctcgaaca
     1201 gggccacgtc gaacgtcagc ttgtggcccg gcgcctggtc gcggatggcg accgggatgc
     1261 gcgggcgccc ctccgcctcg aagtagcggg cgtaccagcc cgaaaagatg aaattggagc
     1321 cgacgatgct gacgtccttc cgccgcccga ggaccatctg ctgataccag aggcaatagg
     1381 tatcggcgtc gccgccggtc agcacgaccg cgccgtcggg cagcgcgttc atcacggcct
     1441 cgccccagac ggccggcgcg tcgtcccacg atttgttgag acgtgggtaa tgccatagca
     1501 gcatggcgcc ggcgacgagc accggcacgg cgcgcgagag cggcccgagc ggaagttcgc
     1561 cccaccggcg gccgagcgcc gccagcgccc aacgcacgct cacgatccag ccgagcgccg
     1621 cggccggcgc ggcggggaga aaataagcgt cgatatcggg gatctggtag aacatcccga
     1681 acccggccgt gagcgccagc gtcgcgagca atccggcgcc cagctcccag cgcgcgcgcg
     1741 ccagcaggat caggccgccg attgcgaccg ccagcacgat caggcccact accaggcaga
     1801 ccccgggggt cgcgtaagcc cccggcgcca gttgatagcc ccaccagccg atccagccga
     1861 gcagcccgcc ggcggccgag ccctggtcgg cgcccatccg cagctcgcgg aactggccgc
     1921 cgctgagaac ccagatcaga ttgcgcagcg aactgggatc gccccagttg agcggcggat
     1981 tggcccgcgc tcgcagaggc agatacagat agcccgccag tggaataaca aaaccggcca
     2041 cgccggcgag cgggatccgc cacggttgcc ccgcgcccct gccccagcgt agcaggacca
     2101 gagccgccag cggcgcgaac gcgacgcagg aataatgatt caggcccatc agcccgagca
     2161 tgacgcccgc cgccgcggca tggcgcggcc cgcgcggggc gccgtcgtgc cacaccgcga
     2221 gcagcaggcc cgccgccagc acatagttca gggcgtagac ctcggcaaac gtcgcctgct
     2281 cccagaccgt ccgcgacagc gccacgatca gggccgctcc agccgccggc agcgcggcca
     2341 tgacccccgc gcgggccctc tgatccgccg ggttcgccgg gtcacggaca agcgtttgct
     2401 gaaacaggcg aaacaacagg ccggccgcga ccgcctgaca gacgacgttg aacagcgtca
     2461 gtgcgcgacc cggatccagg tgcgccagcc ccttcaacca gaggtgtccg agcaccgtgt
     2521 agagcggata gccggtcggg tgggggatgc ccagcgtcca tgcggcgcag gcgagttcct
     2581 ggccgtcgcc ccaccatgcg ccgggggcgg ccgtcgtcag gtaaatcaat ccggcggccg
     2641 ctgcgaccgc gccggccacg gcccgaccgg cgcccgcgcg cggcggcggc gtgggctcgt
     2701 gcgggagcgc cgcgccagcg cccgcctgcg gcgcggcctg atccgcgcga cccggcttct
     2761 tctttccgtg cgccatcggt aggtcctggc ttggttctcc gtgtttgtcg tcaggcttcg
     2821 gctgctggcg tgtcggcctt aatcgcgatc gtcccgcgtt tgtttgctcg gctgcaagcg
     2881 cagttcgatc ggtcccgtcc ggaacggatc gggcccctgc ccttcatcca ccacccacac
     2941 ggcgaaccgg tgcggcccgt ccccccgcaa ctgggcgtcc tcctcgtcgt ggggcgtcac
     3001 cagcagccgg tgaaattcca cgaaccagaa gccgtcctcg gcatggtcaa tctcgtcgct
     3061 gaaccagtag cccggcgtcc gccggcggcc gtagcggaaa tacgcgctgg gctgaaaatc
     3121 acgaaccttc gcatagagcg ccagggcttc cacgtcggcc tggctcccgc tggcgaagtc
     3181 cgcggccgtt cccggcatcc gctcctcttc gcgcagcagc ggtcgcgtcg tcgggcgcgt
     3241 ccccggctga cccgtgtcag gaatcacctg ccgctcgatc caccggtcgg tccccggcac
     3301 gggaaacagc tcaccctcca ccgtcgcggg atcggtcccc cgggggtgct ggcgcaggag
     3361 cagccgggcg tcatcggcgt ggcccgacag attcgtccag ccgccgcgcc agcgccacag
     3421 atcatagcct tcgggctgtt cgggaaccgc ggcgaacgtc gggtcggggc tcatcggcca
     3481 gaggagcgcc acttcgtcgt tcggccggct cacccagcgg tactcatggc gctgctcatc
     3541 ccagacccat acagggggcg tgtcgccttc cgagcggtcg ggccacgaga tcagcagccc
     3601 cagcgcgcgg ccgtcatgaa tcgcgcgcag gcgcaccggc agccggacgc cagcgggcgt
     3661 ccgcgcggtc acctcgattt ccttggcgcg gccccagacg gccggttcga taatccggtt
     3721 cggcagggcg acctggtcca cgcgcaaggc gtgcaggcgc ggctggcgcc aggcgcaacc
     3781 tgccgcggcg agcaacccgc ccagcagcgc cgcgcgcccg gctgctttca ggaaccggcg
     3841 gaacataacc gctcaacctt cgatcgatca tgggattgaa atgcgggaga cgcgtgtggc
     3901 atgggcgatc ttgcacggtc aggcaagggc caccgcttcg accgcgcgcg gatcgatccg
     3961 agcgaccggc gggacacgac acatcgatgg gcgggcggct gctgcgtcgc cagcggccct
     4021 tccaacttga tccgtgccgc ctcgcgaccc atcccgtgaa tatcctgctt cagcatcaca
     4081 gcgggccggc ccttcagccg ttggccgccg cggccgccgc cggcgtcaga cgccggtctt
     4141 ttcctgcaac ttgacgaaat aatcctccgg atcgaacaaa acttcgcgcg cgaatttctc
     4201 ccacagctcg atctgattga agctcggcgc gagcaggccg gggggaagcg tccggtagat
     4261 ctccaccgcc gaatcgtaga gcgtcagatc ggtcaggctc tcatcgaacg gccgcggccg
     4321 cggcggcgcc gtcaggccca ggtcatgcag cgcctcggcg atgatccggt tgacctcgac
     4381 catctgaaac acccgccggc gcccgtcggt ttccgccagg acggcatcca gccccggcgc
     4441 gatccggtcg acatacttcg gcagccgacg gaatgtccgg ttgagccatt tggtatacgg
     4501 cccgtagcgc cggttgagca tgtaacacat ttccatcacg cgcttgaaat actgccccag
     4561 atagatcagg ccggtcagtt cgtcggcgcg cttgacggcg cggttgtagt tgtagggcgc
     4621 ctcgcggccg gtgatgtaca tgcaccagtc cgcgatccgt tttttccaga tggcgtcggg
     4681 ataatatgcc agatgctcgc ggcggcgcgt cagttcgccc ggcccgtcat ggacgatcgc
     4741 gccgctggtc acatgcgaca ggtcgacttc gcacagcgcc agccagtcgg catcggtccg
     4801 cggcagttga tccgtcccca ggaaccgggc gaagaagtcc tcaaccacat tggcgcacag
     4861 gcccagcata ccggtccggt cgtagcggat ggaataatcg cggaaattgt ccggcggccg
     4921 gttttcgagc gccgcgacca gccgcggcaa tagccgatcc gcatcggcgc ggcacagcat
     4981 gatgttcgcg cgcggcgacc agtgatgatc gcggctgaga tcgtcatcga gtcccagcac
     5041 gtcggagccg gtgccgatga ccgccacggc cacccggtcg aaatcgtcgc ccagctcgtg
     5101 gcggagccag ggcatgacga cctcacggca gaagtcgacg ctgatttgaa ggccggaaac
     5161 catggtgcgc ttcctccgcc cggagcggcg ctcggcgggc ggagccaccg ccgcccggat
     5221 tcaagatgtc gaacggatcg tccggcgcct cgacaaccag gccggtctca ggcgcttcca
     5281 cgcgcggaag cgctcacgct tcaggagacg cccgccgcgg ctttccgctc ggcggcccac
     5341 gccgcgggca ccggaaccgg gttgatcccg cgcagcatca gcccctgccc gcgcgaggcg
     5401 tggatgtcgc gcgtcggcgg atcgctcgga tcgcgcatct gaatcaggat gttgcgccgc
     5461 ggccgcccgc tgcggttcgg actcgatccg tggatcgtca ggtaattgaa aatcaccccg
     5521 tcgcccgcct tcgccttgac cggcagaccc tcctcgatcg ggtacttctc cggtggcagg
     5581 taaaatcccc ccggatcgca ttcgagcggg ccgagcttgt gcgatcccgg catgacgcgg
     5641 atgcagccgt tttcctcatc cgcgtcgtcc aggtagatgc tcacggcgaa catcgtgtgg
     5701 ttctggtgcg gaaaatacgg gtaatcctgg tgcatcggga aggcgccccc cttttcggga
     5761 ggcttggcca gcatcttggt gtgatgcagc tgcacgttcg gtccgatcag cgcctcggcg
     5821 atgtcgagca gcttgtcgtt gaacagcagc ttgccgaata tggcggcctg aaactgcatg
     5881 tcgtgaacgg cattcagccg ctgctgggcg cgctcctcgt ccttcagcca gttccccttc
     5941 catagattgt tggcctcctg ctgatggcgg ctcatccgct cgatcgtcgc atcggcttcc
     6001 tcgcgcatgg cctcaacttc ggccggggtc agcaaaccct cggccagcag gtagccatgc
     6061 tcgcgataga atgcggccat ttcgttcaga ttcatgagca actcccttgg aatccccgga
     6121 ggggtgagat cagcctgact cgcggccgag gcaccggccg cgggtcggtc gcgagtgaac
     6181 ggtgc
//
LOCUS       DAOPCO010000241         6148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11236_length_6148_cov_4.953389, whole genome shotgun sequence.
ACCESSION   DAOPCO010000241 DAOPCO010000000
VERSION     DAOPCO010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11236_length_6148_cov_4.953389"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLS90_13785"
     CDS             <1..241
                     /locus_tag="PLS90_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03514.1"
                     /translation="GLLERYQQHIIAERRAELLRQLQEERPIEVFADRLQAMVDGGTT
                     QTLATTPTLSLDPTGAPAPADDAPAPPDNESADPE"
     gene            238..1251
                     /locus_tag="PLS90_13790"
     CDS             238..1251
                     /locus_tag="PLS90_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03515.1"
                     /translation="MTRRRITPRPWIVVALLALFGAAVRGQSVPEFRVEAEYIDRPEA
                     LYVGERALLTLVTEPADFPVDRILHVRFEPDQELWHLDRNWHLTESPDPEAEGGAVRR
                     WRAQIRPYRVGQLTLPAITVRLRLDDGTTTETVIDDATLDVASIIPEGAETVLLPLRD
                     PVDIPFDWTWVYIILGALVIVLALAYLIARWWARRGATVQAPVEPPLPPGLWALRELD
                     QRSALSVCRTGPPKIIFTHVSEVIRLYLQRRYGFAAIDMTTLECLRALHDHNPGAQVE
                     RWVQEFLDECDMVKFTRFDPPRERWATIWNDARLIVKMTTPPEELGEAGQTVDPLAEE
                     ATG"
     gene            1248..2237
                     /locus_tag="PLS90_13795"
     CDS             1248..2237
                     /locus_tag="PLS90_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK03516.1"
                     /translation="MIRFNDPWVLLLFIPLLLLGAWLWRRRRLRPRLRFSSTELLDEV
                     GPTLRTRLLGLPAILLFVAAALLTVALARPQSAWREHRRFTEGIAIMLVLDVSDSMMA
                     LDFEPNRLERAKAVVQEFIQGRENDQIGAVIFGRDTFTLCPLTQDYQALRNFVQRINF
                     DLVNGEGTAIGMGLANAVDKLRKSPNKSKVVILLTDGENNAGQIDPIDAAEIARQFDI
                     RVYTIGVGSVGGQVLIPNPSGFGPRYIPIEARLDVEQLTRMAEMTGGRFFHATDGSSL
                     EKIYQQINEMERTEIEVHETHYFDELAAWLMVPALLILLLALGLENTWLRTFP"
     gene            2280..3302
                     /locus_tag="PLS90_13800"
     CDS             2280..3302
                     /locus_tag="PLS90_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPK03517.1"
                     /translation="MHWGNPQMLWFLLAWPLLAWLGWAALRRRARLVARLGEHSLLGR
                     LFPASVQRWRRRRLLRLLLAAILLTIAAARPQYGQIEQTLRSFGTHVIVAVDVSRSMK
                     AADVPPNRLEAAKHSLTLLLRRLEGHRVGIIAFAGDAFLQCPMTLDREMATLVLRSLD
                     TDSVGALGTNLEVLIETAMEAFERDGVDGSSAVVLLTDGEELEGNAIDAASRAAARGI
                     RIYPIGIGTDAGAPILLEENRFLEDAQGAKVNTRLRMDTLQRIAELTGGEAFEAGDNP
                     LPAVTAVARLIEDQEKSEIESRRRIIYEDRFQWFLAPALALIFWVLASRPEPTRAGRP
                     FAAEGS"
     gene            complement(3297..4742)
                     /locus_tag="PLS90_13805"
     CDS             complement(3297..4742)
                     /locus_tag="PLS90_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPK03518.1"
                     /translation="MLRETYPYYLANRAAVPNHALEVTDKYTGKVATRVALANPAAIE
                     RAIAAAVEAASAMARLPHWKRQAILLRCVAEFEARREELAEALCVEAGKPIRDSRGEV
                     GRLIDTFRIAAEEAVRIGGEVLPLDISARAEGYRGMWKRVPVGPCSFISPFNFPLNLA
                     AHKIAPAIAAGCPFVLKPASRTPVGALLIGEILAETELPAGAFSILPCAREHADPFVE
                     DDRLKLLSFTGSDEVGWELKRRAGKKKILLELGGNAACIVDEDVDLEDAVGRVIVGAF
                     YQTGQSCISVQRLIVHEQVYDAFRDRLVAETRKLKVGNPLDEETFIGPVISEAAAERI
                     EQWIREAERAGGRILCGGRREGVMVEPTLMENVPRDQKLVTQEAFGPVAVLSCFSKFE
                     EALREANDTRFGLQAGVFTRDLYRAQRAWDELQVGGVVIGDVPSWRVDHMPYGGVKDS
                     GLGREGIRNSIEAMTEIRLLVIRTPPGEGGS"
     gene            complement(4845..>6148)
                     /gene="der"
                     /locus_tag="PLS90_13810"
     CDS             complement(4845..>6148)
                     /gene="der"
                     /locus_tag="PLS90_13810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPK03519.1"
                     /translation="FNRIIGRRKAVVHDRPGVTRDRNLDVADWLGRPFLCVDTGGFDT
                     ELDDPLLENVVEQARMAIDEADVIILLFALGESAHPADAEMVRLLRTTRKPVLAAVNK
                     CDNEREELESADFYQYGIDRLYPISALHGRGVGDLLDDVVAALPATPAGDRGGAAEGA
                     GRPIALAIVGRQNVGKSTLINRLAGEPRVIASPVPGTTRDAIDTLVTAPGGRPFILID
                     TAGIRRRGKIERGVERLSVMSAMLSLRRADVAALVIDAAEGITEQDAHVAGYGLEAGL
                     PTMILVNKWDLVEKDHRTADEFTKRLEKEWQFLRYAPVLYMSGLTGQRATRVYEIAER
                     VYENSTRRVPTPALNEKLAEWVERKPPAMKRNRRPKVRYGAQVGVQPPRFAVFVNDPQ
                     LFHFSYERYLVNRIRETWDFEGTPIKLHFRRSSRRKGEGED"
BASE COUNT          966 a         2336 c         1807 g         1039 t
ORIGIN      
        1 ggggctgctt gaacgctatc agcagcacat catcgccgag cgccgcgccg aactgctgcg
       61 ccagctccag gaggaacggc ccatcgaagt tttcgccgac cggcttcagg caatggtcga
      121 tggcggcaca acgcaaaccc tggcgacgac gcccaccctg tccctcgatc cgaccggcgc
      181 ccccgccccc gcggatgatg ccccggcgcc gccggataac gagtccgccg atcccgaatg
      241 acccgccgcc gtatcacccc ccggccatgg atcgtcgtgg ctctgctcgc cctgttcggc
      301 gccgccgtcc gcggccagtc cgtgcccgag tttcgtgtcg aagccgaata catcgaccgt
      361 cccgaggccc tgtatgtcgg cgagcgcgcg ctgttgaccc tcgtcaccga accggcggat
      421 ttccccgtcg accgcatcct ccacgtccgt ttcgagcccg atcaggaact gtggcatctc
      481 gaccgcaact ggcacctgac tgagtcaccc gatcccgagg ccgaaggcgg cgcggttcgg
      541 cgctggcggg cccagatccg accctatcgc gtggggcaac tgacgctgcc ggccatcacc
      601 gtgcgcctcc gcctggacga cggcacgacg accgaaaccg tgatcgatga cgccacgctg
      661 gatgtggcca gcatcatccc cgagggcgcc gaaacggtcc tcctcccgct gcgcgacccc
      721 gtcgacatcc cgttcgactg gacctgggtt tacatcattc tcggcgcgct ggtgatcgtg
      781 ctggcgctgg cgtatctgat cgcacgctgg tgggcgcgcc gcggcgccac cgtgcaggcg
      841 cccgttgaac ccccgcttcc ccccggcttg tgggccctgc gcgagctgga tcagcgctcc
      901 gccctctcgg tctgccgcac cgggccgccg aagatcatct tcacacacgt ctccgaagtc
      961 atccggcttt acctccagcg ccgctacggg ttcgccgcca tcgacatgac caccctggag
     1021 tgcctgcgcg ccctgcacga tcacaatccc ggcgcccagg tggagcgctg ggttcaggag
     1081 ttcctcgacg agtgcgacat ggtcaagttc acgcgtttcg atccgccccg cgagcgctgg
     1141 gccacgatct ggaatgacgc gcgcctgatc gtgaaaatga ccaccccccc cgaggaactg
     1201 ggcgaagccg gccagaccgt tgatccactg gccgaggagg cgaccggatg attcgcttca
     1261 acgatccgtg ggtcctgctg ctctttatcc ccctgctgct gctcggcgca tggctgtggc
     1321 gccggcgccg gctgcggccg cgcctgcgct tttcctccac cgaactgctc gacgaggtcg
     1381 gcccgacgct gcgcacccgg ctgctcggcc tccccgcgat cctgctcttt gtcgcagccg
     1441 cgttgctcac cgtcgccctc gcccgcccgc agagcgcatg gcgcgagcat cgccggttca
     1501 ccgaggggat cgccattatg ctcgtgctcg atgtcagcga ttcgatgatg gcgctcgact
     1561 tcgaacccaa ccgcctggag cgcgccaagg ccgtcgtcca ggaattcatc cagggccgcg
     1621 aaaacgatca gatcggcgcc gtgatcttcg gtcgcgatac cttcacgctc tgcccgttga
     1681 cgcaggatta tcaggcgctg cgcaactttg tccaacggat caatttcgac ctggtcaatg
     1741 gcgaagggac cgccatcggc atgggactgg ccaatgccgt cgacaagttg cgcaagtcgc
     1801 ccaacaagag caaggtcgtg atcctgttga ccgacggcga gaacaacgcc ggccagattg
     1861 atccgatcga tgccgccgaa atcgcgcgcc agttcgacat ccgcgtctac accatcggcg
     1921 tcggcagcgt cggcggccag gtcctgatcc ccaatccgag cggcttcggc ccgcgttaca
     1981 tcccgattga agcccgcctg gatgtcgaac agctcacccg gatggccgaa atgaccggcg
     2041 gccgcttctt ccatgccacc gacggctcct cgctggagaa aatctaccag cagatcaacg
     2101 aaatggagcg caccgaaatc gaggtccacg agacccacta cttcgatgaa ctcgcggcct
     2161 ggctgatggt ccccgccctg ctgatcctgc tcctggccct gggcctcgag aacacctggc
     2221 tcaggacgtt tccatgagcc cgcccgacgc catcatcccc ggggagcgat tcgccgcgca
     2281 tgcattgggg taacccgcag atgctctggt tcctgctggc ctggccgctg ctggcgtggc
     2341 tcggctgggc cgccctgcgc cgccgcgccc ggctggtcgc ccgcctgggc gaacattccc
     2401 tgctcggccg gctctttccc gcctcggttc agcgctggcg gcggcggcgt ctgctgcggc
     2461 tgttgctcgc cgcgatcctg ctcaccatcg ccgcggcgcg cccgcagtac ggccagatcg
     2521 aacagaccct gcgcagcttc ggcacgcatg tgatcgtcgc cgtcgatgtc tcgcgctcga
     2581 tgaaagccgc cgacgtcccg cccaaccggc tcgaagccgc caagcacagc ctgaccctcc
     2641 tgctgcgccg cctcgagggc catcgcgtcg gcatcattgc cttcgccggc gatgccttcc
     2701 tccagtgccc gatgacgctc gaccgcgaga tggccacgct cgttctgcgc tcgctcgaca
     2761 ccgattcggt cggcgcgctc ggcaccaacc tggaggtcct gatcgaaacg gcgatggaag
     2821 ccttcgagcg cgacggcgtc gacggcagca gcgccgtcgt cctgctcacc gacggcgagg
     2881 agctcgaagg caacgccatc gacgccgcca gccgcgccgc cgcccggggc attcgcatct
     2941 atccgatcgg catcggcacc gacgccggcg cccccatcct gctggaggaa aatcgcttcc
     3001 tcgaggacgc tcagggcgcc aaggtcaaca cccgcctgcg catggatacg ctgcaacgca
     3061 tcgccgagtt gaccggcggg gaggccttcg aagcgggcga taatccgctc cccgccgtta
     3121 ccgcggtcgc gcgcctgatc gaggatcagg aaaaatcgga gattgaatcg cgccgccgga
     3181 tcatctacga ggatcgcttt cagtggttcc tcgccccggc cctggccctg atcttctggg
     3241 tcctggccag ccgtcccgag cccacccgcg ccggccgccc gttcgccgcc gagggctcat
     3301 gagcccccct cgccgggcgg cgtgcggata acgagcagcc ggatttccgt catcgcttca
     3361 atcgaattgc ggatcccttc gcgccccagc ccgctgtcct tcaccccgcc atacggcatg
     3421 tgatcgacgc gccagctcgg cacgtcgccg atcaccacac ctcccacctg cagctcatcc
     3481 caggcccgct gtgcgcgata caggtcgcgc gtgaagaccc ccgcctgcag cccgaaacgc
     3541 gtgtcgttgg cctcgcgcag cgcctcctcg aacttgctga agcacgacag cacggccacc
     3601 ggcccgaacg cctcctgggt caccagcttc tggtcgcgcg gaacgttttc catcagcgtc
     3661 ggctccacca tcaccccttc gcgccggccg ccgcagagaa tccgcccgcc ggcccgctcc
     3721 gcttcgcgaa tccattgctc gattcgttcc gccgccgctt ccgaaatgac cggaccgatg
     3781 aacgtctcct cgtccagtgg attgcccacc ttcagcttgc gcgtttccgc caccagccgg
     3841 tcgcgaaacg catcgtacac ctgctcgtgc acgatcagcc gctgcacgct gatgcagctc
     3901 tgccccgttt ggtagaacgc gccgacgatc acccgcccga ccgcatcctc caggtcgacg
     3961 tcttcatcga cgatgcacgc cgcgttgcct cccagttcca gcaggatctt cttcttgccc
     4021 gcgcgccgct tcaattccca gccgacctcg tcggaaccgg tgaagctgag cagcttgagc
     4081 cggtcgtcct cgacaaacgg atcggcgtgt tctcgcgcgc acggcaggat cgagaaggcg
     4141 cccgccggca gctcggtttc ggccaggatt tcgccgatca gcagcgcgcc gaccggcgtg
     4201 cggctggccg gcttcagcac gaacgggcag cccgccgcga tcgccggcgc gatcttgtgc
     4261 gccgccaggt tgagcggaaa attgaacggc gaaatgaacg aacagggacc gaccggcacg
     4321 cgcttccaca tcccccggta gccctccgcc cgtgcgctga tatcgagcgg caggacctct
     4381 cccccgatcc gcaccgcctc ctcggccgcg atccgaaacg tgtcgatcag ccggccgact
     4441 tcaccccggc tgtcgcggat cggcttgccc gcttcgacgc agagcgcttc ggccagttcc
     4501 tcccggcgcg cctcaaactc cgccacgcag cgcagcagga tcgcctggcg cttccagtgg
     4561 ggcagccggg ccatggccga cgcggcctcg acggccgccg caatggcccg ttcaatcgcc
     4621 gccggattgg ccagcgcgac ccgcgtcgcc accttgccgg tgtatttgtc ggtaacttcc
     4681 agcgcgtgat tcggcaccgc cgcccggttc gccagataat acggatacgt ttcgcgcagc
     4741 atggctctgc tcccaccaaa ggctttgatt tcggtgggta gcataacccg ttccggtcgg
     4801 ctccaccagc cccgccgccg cgccggcccc gtccgcggca aacctcagtc ctccccttcc
     4861 cccttgcggc ggctgctgcg ccgaaaatgc agcttgattg gcgtcccctc gaaatcccac
     4921 gtctcgcgaa tgcgattgac caggtagcgc tcataagaaa aatggaacag ttgcggatcg
     4981 ttcacaaaca cggcgaagcg cggcggctgc acccccacct gcgccccgta gcgcaccttg
     5041 ggccgccggt tgcgcttcat cgccggcggc ttgcgctcca cccattcggc cagcttctca
     5101 ttcagcgccg gagtcggcac gcgccgcgtg gagttctcat agacccgctc ggcgatctca
     5161 tacacccgcg tggcccgctg acccgtcagc cccgacatgt agagcaccgg cgcatagcgc
     5221 agaaactgcc attccttttc caaccgtttc gtgaactcgt ccgcggtgcg gtggtccttt
     5281 tccaccagat cccatttgtt gaccaggatc atcgtcggca ggcccgcctc caggccgtag
     5341 ccggcgacat gcgcgtcctg ttcggtgatc ccttcggccg cgtcgatcac aagggccgcc
     5401 acgtcggcgc gccgcagcga gagcatcgcc gacatcaccg agaggcgctc caccccccgc
     5461 tcgatcttcc cccgccggcg gatgcccgcc gtatcgatca gaatgaacgg ccgacccccc
     5521 ggcgcggtga ccagcgtatc gatcgcgtcg cgcgtcgtcc ccggaacagg cgacgcaatc
     5581 acccgcggct cgcccgccag tcggttgatc agcgtcgatt tgccgacgtt ctgccgcccc
     5641 acaatcgcca gcgcgatcgg ccgcccggcc ccttccgccg cgcccccccg gtcgcccgcc
     5701 ggcgttgcgg gcagcgccgc cacgacatcg tccagcagat cgccgacgcc gcgtccgtgc
     5761 agcgcgctga tcggatacag ccggtcgatc ccgtactggt aaaaatcggc cgactccagt
     5821 tcctcccgct cgttgtcgca tttgttgacc gccgccagca ccggcttgcg cgtcgtccgc
     5881 agcaggcgca ccatctccgc gtcggccggg tgcgccgact cccccagcgc gaagagcagg
     5941 atgatcacgt cggcctcgtc gatcgccatc cgcgcctgct cgacgacatt ttccagcagc
     6001 ggatcatcca gttcggtgtc gaatccgcca gtatcgacgc acaggaacgg gcggcccagc
     6061 cagtccgcca cgtccagatt gcggtcgcgc gtcacgccgg gccggtcatg caccaccgct
     6121 ttgcgccggc cgatgatccg gttgaaca
//
LOCUS       DAOPCO010000242         6147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11239_length_6147_cov_3.885259, whole genome shotgun sequence.
ACCESSION   DAOPCO010000242 DAOPCO010000000
VERSION     DAOPCO010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11239_length_6147_cov_3.885259"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="PLS90_13815"
     CDS             <1..1374
                     /locus_tag="PLS90_13815"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010493722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPK03520.1"
                     /translation="GGPFLTLPLVLTRSPADGTPNLGIYRMQRYDDAELGLHIQIGKG
                     GGFHFWEAEQRGEPLPVTVFLGGPPALILAAVAPLPEGVPELLLASLLLGERLPMTNS
                     PLSPHPLVAEAEFALLGHAPPGVRRPEGPFGDHYGYYSLRHDYPVFRCEAIHHREGAI
                     YPATIVGKPRQEDYYIGEYLQRLLRPLFPLVMPAVRDVWSYGETGFHALAAAVVRERY
                     PREAMSAAFRILGEGQLGLTKFLLAIDQPMDLTDFPAVLEHVLARFRPETDLFVFANL
                     AMDTLDYTGPRVNEGSKGVMLGLGEPVRDLPREWRGDPPAGTFNPHVFCAGCLVLGAP
                     PFPPRDLPDPLPPAAAQSPALADWPLVILCDDPARATASTINFLWTVFMRFEPAADIH
                     AAAADIRRNHICYHGPIVIDARMKPWYPAELFAAPAIAQRVEARWREYFPAGNVAMGS
                     SDRAHLQ"
     gene            complement(1539..2105)
                     /gene="amrA"
                     /locus_tag="PLS90_13820"
     CDS             complement(1539..2105)
                     /gene="amrA"
                     /locus_tag="PLS90_13820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPK03521.1"
                     /translation="MKQELDPAEQQWLSRLAGEALRDAVTGRPVSAVDREALAAAAPR
                     LLEPAGAFVTVYVDGELRGCLGEVDPAEPLVEVARRCAARAARHDYRFAPVTPDEWPG
                     VTWKISVLTRPVPIQDPAEIVIGRDGLIAEHGVQRGLLLPDVASERGWDVETFLVQLR
                     RKAGIDAEVPWSEIWLERFETQIIEGRP"
     gene            complement(2137..3426)
                     /locus_tag="PLS90_13825"
     CDS             complement(2137..3426)
                     /locus_tag="PLS90_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03522.1"
                     /translation="MAAWLLALALGAGAQEAAAPADESEAVLNLENEPGGLVAPYQSF
                     LYLIHRYFPDASGRWDPASEVMRLHIKDLEIEVPAARPEVIVNGEVRRVERSLILRQG
                     EVKVPLATVELILRELEVPYEFAPGEGEEPAARRAEPPPVPTDLIETTPETTPEATPE
                     EPPRPARDDAAVSPPAVEITPAPEPELVNPPAVLLPSDETAAPAPIQREEAMALQPPP
                     ALAGKIGLTWAQLTDLDHRRPPRRVTLAFDDGLADLAGRVEQKLRDGLGLVVTRVPIG
                     AGRRSSDVLASQINGSRPELFLDLVANPVLPEQAPGSQPLIVWVVNEALWPNDRGQPP
                     GEAGSIHAYLRHEFQSLALGSLLRNALGQQSGAAEGVQYELAPSYLLRRVDSASAAVL
                     VPLGEAREDEANRMELIARGIQAAVEGYTRGMSQAQF"
     gene            4106..4804
                     /locus_tag="PLS90_13830"
     CDS             4106..4804
                     /locus_tag="PLS90_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK03523.1"
                     /translation="MLLRFEQVVRQFPAAEPGAPPRRVLDGVDFSLEPGQVVALVGRS
                     GAGKTTLLHLASGLDQPDAGHVWFDGRDLAAMTPAQTSRLRRHRIGLVFQNNLCLNAL
                     PVWENVALPLLMTHTQRRQARRQAEAILEQVGLEGLADASTAVLSGGQRRRLGIARAM
                     LGRPDLLLADEPTADLDEETGARIERMLFDWLLAERRAALIVTHNESICRAAHHTFRI
                     RDGRLTEAASLDIL"
     gene            4858..>6147
                     /locus_tag="PLS90_13835"
     CDS             4858..>6147
                     /locus_tag="PLS90_13835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HPK03524.1"
                     /translation="MNDVRQPFVLPRPQVPWWRDQARALRRAYVRSRRERAAAEQLKR
                     WNSELNWREFWHGRLFLESYPRIIQVGTNWTCNLKCSFCRLTMDWTQAQLRRLPAREL
                     AISDRVQETVERLLPYAEMMVLTPLGEPLLWAGLKPLLELHARVGSHNLALTTNGMLL
                     NERNVERLIEGQLSQLYVSIDSNDPEIYPTLRVGGDLATVEAGVRRLVARRRASGAPW
                     PRLTMNATFMRRNIEQLPSMVRWAHELGFDEISVQLMEIENPEHEPEFLGHFPDLARE
                     MVLRALAVGREVGLIVLPHLALRNLISAAGEGHNVADHQFQGASPIMPGKPDLAGAPD
                     AKGSQPARPSGAHAAASGCADCHAAAPPFADALIDPALDMRHKTLVEKCHYPWYNLLI
                     DTDGDARPCCWADASWGNLNQLSFEEIWNGERAQAMRR"
BASE COUNT          919 a         2547 c         1670 g         1011 t
ORIGIN      
        1 ggcggcccct tcctcaccct cccccttgtg ctgacccgca gcccggccga cggcacgccc
       61 aacctgggca tctaccgcat gcaacgttac gacgatgccg aactgggcct ccacatacag
      121 atcggcaagg gcggcggctt tcacttctgg gaggccgaac agcgcggcga accgctcccg
      181 gtcactgtct tcctcggcgg cccccccgcc ctgatcctcg cggccgtcgc ccccctgccc
      241 gagggcgtcc ctgaactcct gctcgcctcg ctcctcttgg gcgagcgcct gccgatgacc
      301 aactcccccc tctccccgca cccgctcgtc gccgaggccg agttcgccct cctcggccat
      361 gccccccccg gcgtccgccg ccccgagggc cccttcggcg atcactacgg ctattactcg
      421 ctgcgccacg actaccccgt cttccgctgc gaggcgatcc accaccgcga gggggcgatc
      481 taccccgcca ccatcgtcgg caagccgcgc caggaggatt actacatcgg cgaatacctc
      541 cagcgcctcc tgcgccccct cttccccctc gtcatgcccg ccgtgcgcga cgtctggtcc
      601 tacggcgaga ccggcttcca cgccctcgcc gcggcggtcg tccgcgaacg ctacccccgc
      661 gaggcgatgt cggccgcctt ccgcatcctc ggcgagggtc agctcggcct caccaaattc
      721 ctgctcgcca tcgatcagcc gatggacctg accgactttc cggctgtgct ggaacacgtc
      781 ctggcgcgct tccgccccga gaccgacctg ttcgtctttg ccaacctcgc gatggatacc
      841 ctggattaca ccggcccccg cgtcaacgaa ggctccaagg gcgtgatgct cggcctgggc
      901 gagccggtgc gcgatctgcc gcgcgagtgg cgcggcgatc cccccgccgg gacgttcaac
      961 ccccacgtct tctgcgccgg ctgtctcgtc ctcggcgcgc cccccttccc cccccgtgac
     1021 ctgcccgacc ccttaccccc cgcggcggcg caatcccccg ccctggccga ctggcccctg
     1081 gtcatcctct gcgacgaccc ggcccgcgcc accgcttcca cgatcaattt cctctggacc
     1141 gtcttcatgc gcttcgagcc ggccgccgac atccatgccg ccgccgccga catccgccgc
     1201 aaccacatct gctaccacgg cccgatcgtc atcgacgccc gcatgaaacc gtggtatccc
     1261 gccgaactct tcgccgcccc cgcgatcgcc cagcgggtcg aggcgcgctg gcgcgagtat
     1321 ttccccgccg gcaacgtcgc catgggctcc agcgaccgcg cccacctgca gtaacacccc
     1381 cggcccgcgc tcggcccttc cgttctttat gtcctccccc ctccaggaaa aacgaggccg
     1441 accgcgtcac cccttcgcgt cccggggttt ccgtatttac atttgtggat tcgaatgtgt
     1501 ttcgaagttc gacattcgaa tttcgtgctt ctttccgcct acggccgacc ctcgataatc
     1561 tgcgtctcga aacgctccag ccagatttcc gaccacggca cctccgcgtc gattcccgcc
     1621 ttgcgccgca actgcaccag aaacgtctcc acgtcccatc cccgctccga tgccacgtcc
     1681 ggcaacagca gcccccgctg cacgccgtgc tcggcgatca gtccatcccg cccgatcacg
     1741 atctccgccg gatcctggat cggaaccggc cgcgtcagca ccgaaatctt ccaggtcacc
     1801 cccggccatt catccggcgt caccggcgca aaacggtagt catgccgcgc cgcccgcgcc
     1861 gcgcaccgcc gcgccacctc caccagcggc tccgccgggt ccacttcccc taaacacccg
     1921 cgcagctcgc catcgacata caccgtgacg aacgcgccgg cgggctccag cagccgcggc
     1981 gccgcggccg ccagcgcctc gcggtcgacc gccgataccg gccgccccgt cacggcatcc
     2041 cgcagcgctt cccctgccag ccgactcagc cattgctgct ccgcgggatc aagctcttgt
     2101 ttcatcggcg ggcgtccttt ccgcctggct ggttcgctag aactgcgcct gactcatccc
     2161 ccgcgtatag ccctcgaccg cggcctgaat cccgcgagcg atcagttcca tccggttcgc
     2221 ctcgtcctcg cgcgcctcgc ccagcggcac cagcaccgcc gccgacgccg aatcgacccg
     2281 gcgcagcaga taggacggcg ccagctcata ctgcaccccc tcggccgcgc cactctgctg
     2341 ccccagcgcg ttgcgcagca gcgaccccag cgccaggctc tggaactcat ggcgcagata
     2401 cgcatggatg ctgcccgcct cgcccggcgg ctgcccccgg tcgttgggcc acagcgcctc
     2461 gttcaccacc catacgatca gcggttgcga cccgggcgcc tgttcgggca gcaccgggtt
     2521 ggcgacgaga tccagaaaca gctccggccg cgacccgttg atctgcgagg ccagcacgtc
     2581 gctgctgcgc cggcccgcgc cgatcggaac ccgcgtgacg accagcccca gcccgtcgcg
     2641 caacttctgc tccacgcgcc cggccaggtc cgccagccca tcgtcgaacg ccagcgtcac
     2701 ccgccgcggc ggacggcggt ggtccagatc ggtcagttgc gcccacgtca gcccgatctt
     2761 gcccgccagc gcggggggcg gctgcaacgc catcgcttct tcccgttgaa tcggcgccgg
     2821 tgccgccgtc tcgtcgctcg gcagcagcac cgcgggggga ttcaccagct ccggctccgg
     2881 cgccggcgta atctccaccg ccggcggcga gacggccgcg tcgtcccgcg ccggtcgcgg
     2941 cggctcttcc ggcgtcgcct ccggtgtcgt ctccggcgtc gtctcgatca ggtcggtcgg
     3001 caccggcggc ggttccgctc gccgcgccgc cggctcctcc ccttcccccg gcgcgaattc
     3061 atacggcacc tccagctcgc gcagaatcaa ctccaccgtc gccagcggca ctttcacctc
     3121 cccctgccgc agaatcagtg atcgttccac ccggcgcacc tcgccgttga cgatcacctc
     3181 cggccgcgcc gccggcacct cgatctccag atccttgatg tgtagtcgca ttacttccga
     3241 cgccggatcc caacgaccgg aggcgtccgg aaaatatcgg tgaatcaggt agagaaaaga
     3301 ttgatacggc gcgacaagcc cgcccggctc gttctccaga ttgagcaccg cctccgattc
     3361 gtccgcgggc gccgccgctt cctgcgcccc ggcccccagc gccagcgcca gcagccacgc
     3421 cgccatccat cgcattcgcc ttcgcaacgc tcgcctccgg ccccgcttgg tttcgctccc
     3481 tgcacgggtc ctatttcaga gggccggggc ggtttccgca agccaaaaag gggattccca
     3541 cccccgccgg cgcctccgcc gatgggaccc ctctgcacgg gttccaggat cccacaaaaa
     3601 aaatgtaaaa agggatgatt aaggggttga ctttgccgcc aaccttggct agcctacgcc
     3661 acatattgag agttgttgaa cacatgccta caagatattg atcggaggtt cgggaacaca
     3721 atccccacgt ttgctctcaa actcgaaact gcctgaacga aatcccgcga tttggtttgg
     3781 gtggttgccc ttcgtcaacc tggtcggagc gtgggcggtt tgagagcgaa aaaagagcgg
     3841 cctgagtcga gcggagcgtt cgctccttcc ctctatctcc ttcgggagcc ctccttcgac
     3901 ttgccaaacg tgctgtcagg ttccacggcg tctcccccct gccgtgggtt gcaacctgga
     3961 ctccgtctcg atttcaggcc gctctttagt ctccaacgcg gcgccggtcc ccgcccaccg
     4021 gcgccgcttt cgttttccag gcgtcgcccc gcaccgcccc gccgactccg tttgctatca
     4081 cccccccgac gccctacaat cagccatgtt gttgcgcttt gaacaggtcg tccgccagtt
     4141 cccggccgcc gaacccggag ctcctccccg gcgcgtcctc gacggcgtcg acttctccct
     4201 cgagcccggc caggtcgtcg ccctcgtcgg ccgctccggc gccggcaaaa ccactctgct
     4261 ccacctcgcc tcgggccttg atcagcccga cgccggccat gtctggttcg acggccgcga
     4321 cctggccgcc atgacccccg cccagaccag ccgcctgcgc cgccatcgca tcggcctcgt
     4381 cttccagaac aacctctgcc tcaatgccct cccggtctgg gagaacgtcg ccttgccctt
     4441 gctgatgacc cacacccaac ggcgtcaggc acgccgccag gccgaggcga tcctcgaaca
     4501 ggtcggcctc gagggcctcg ccgacgcttc caccgccgtc ctctccggcg gccagcgccg
     4561 gcgtctcggc atcgcccgcg ccatgctcgg ccgacccgac ctgctcctcg ctgacgaacc
     4621 caccgccgac ctcgatgaag agaccggcgc ccgcatcgag cggatgctct tcgactggct
     4681 gctggccgag cgacgcgccg ccctcatcgt cacccacaac gaatccatct gccgcgccgc
     4741 ccaccacacc ttccgcatcc gcgacggccg cctcaccgaa gccgcctccc tcgacatcct
     4801 ctgacggccc cccctatccc tctttcccgc cgcctcgttt caggctaaac tgacgcgatg
     4861 aacgacgtgc gacaaccctt cgtcctgccg cgacctcagg tcccctggtg gcgcgatcag
     4921 gcccgtgcgc tccgccgggc ctacgttcgc tcccgccgcg agcgcgccgc ggccgagcag
     4981 ctcaagcgct ggaacagcga gctcaactgg cgcgagttct ggcacggccg cctcttcctc
     5041 gaatcctacc cccgcatcat ccaggtcggc accaactgga cgtgcaacct caaatgttcc
     5101 ttctgccggc tcaccatgga ttggacccag gcgcagttgc gccggctccc ggcccgcgaa
     5161 ctcgccatca gcgaccgcgt ccaggaaacc gtcgaacgcc tcctccccta cgccgagatg
     5221 atggtgctca cgccgctcgg cgaaccgctc ctctgggccg ggctcaagcc gctcctcgag
     5281 ctccacgccc gcgtcggctc ccacaacctg gccctcacca ccaacggcat gctgctcaac
     5341 gagcgcaacg tcgaacgcct catcgaaggg cagctcagcc agctttacgt ctcgatcgat
     5401 tcgaatgatc ccgaaattta tccgaccctg cgcgtgggcg gcgacctggc gaccgtcgag
     5461 gccggcgtcc gccgcctcgt cgcccgccgc cgcgccagcg gagccccctg gccccgcctg
     5521 acgatgaatg ccacgttcat gcgccgcaac atcgagcaac tcccctcgat ggtccgctgg
     5581 gcccacgaac tgggattcga cgaaatctcg gtccaactga tggaaatcga aaacccggaa
     5641 cacgagccgg agttcctcgg ccatttccct gacctcgccc gcgaaatggt cctgcgcgcg
     5701 ctcgccgtcg gccgcgaggt cggcctcatc gtcctcccgc acctggccct gcgcaatctg
     5761 atctccgccg ccggcgaggg gcacaacgtc gccgatcacc agttccaggg cgcttccccg
     5821 atcatgcccg gcaagcccga cctcgccggc gcccccgacg ccaagggttc ccagccggcg
     5881 cgaccgtcgg gtgcccacgc cgcggcatcc ggctgcgccg attgccacgc cgccgccccg
     5941 cccttcgccg acgcgctcat cgaccccgcc ctcgacatgc gccacaagac cctcgtcgaa
     6001 aaatgccact acccctggta caacctgctc atcgataccg acggcgacgc gcgcccctgc
     6061 tgctgggccg atgcctcctg gggcaacctc aatcagctct cgttcgagga aatctggaac
     6121 ggcgaacgcg cccaggccat gcgccgc
//
LOCUS       DAOPCO010000243         6133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11266_length_6133_cov_4.331359, whole genome shotgun sequence.
ACCESSION   DAOPCO010000243 DAOPCO010000000
VERSION     DAOPCO010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11266_length_6133_cov_4.331359"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1362)
                     /gene="recF"
                     /locus_tag="PLS90_13840"
     CDS             complement(169..1362)
                     /gene="recF"
                     /locus_tag="PLS90_13840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPK03525.1"
                     /translation="MHVRSLELHHFRNIETLRWAPAPGLNLIWGGNGQGKTNLLEALH
                     CVLTGRSFRTRRDEDCLPWGRRDDPADPTRVDAALQRRAGRRDLRLLIGRRWKRAFVD
                     ATWLPRLGDLWREAAVVTFTPEDAALLKGPPADRRRFLDIVLSQTSAAYLGQLQRYQR
                     ALKQVNAVLRTSPPTRDVRGLAEAYWPELAEAGAHLMCARAAALAEAAEPFAARYREL
                     GGAEPVELVYDANLGRYEINPDGTARPFLDTRRPRSADEPLAAGSGAWRPLTGIYLER
                     LRESYDEGRRHGLCAAGVHRDDFSVRLAGQDLRRFGSQGQHRLVALTLKLESARWIER
                     TLGEPPILMLDDFGSELDPGRRMAVLAGLRGSMQVLITATHPRDLGGEARFDLSAEIR
                     HGTLA"
     gene            1644..2111
                     /locus_tag="PLS90_13845"
     CDS             1644..2111
                     /locus_tag="PLS90_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPK03526.1"
                     /translation="MPAAAPRLIELLDPARIIDLEVETKEEALRRLSQLFQGDPRVLD
                     AEVFHQAILRREQLVSTGVGSGIAIPHVKIPQVTDYVAAVGRLRRGIDFAALDNQPVH
                     LIFMIGASDRQTREFVRILAQVTHLLKDPATRQKLLDAEIPDGFLQIIQQHER"
     gene            2155..3900
                     /locus_tag="PLS90_13850"
     CDS             2155..3900
                     /locus_tag="PLS90_13850"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPK03527.1"
                     /translation="MEITLAGRRLRVSRFTFLLLLGVVVGAVTGCVAVIFRLAAFYGE
                     RLLFPASEHSAIHVIPWLPPWLLRLLMPAVGGLVSGLILYRLLKYAGGHSIPAILQAV
                     ASGQSYFKASMAFPPSMAIVTIATGGSVGPEGPIAEIGSVTGSLIGRWARIPPRLVKT
                     LIGAGVAAGIAAVFNSPIGGVFFAIEVILRNYEIASLTPILVAAVVSSVIAQAALGDR
                     MAIEFPNLGDIPAGELPWFAVLGLLCGALSFLYIHGLGACHGLFRRSGIALWARPMVG
                     GVLVGLIGILFPAVMGEGYEFIRGVIHGSAAEGSVLLLLMLVVLKIAATGFSLGSGNP
                     GGSFAPAVFIGVMAGAAYGYVLRDFRLVQDVAPYAMTGMAGLIAGGLGAPITAILITI
                     RHGGADGPEIILPVMTTVALCFFVMQLQRNVSVYTLEFLRLGIDLDRARQVDPLSLVR
                     VRSVVHTGDFDELPADMPVRQALAQFKESAARWFVVRGEGGQFAGIVSLHEMRLAIAE
                     EELADLLVLADITDSFQPRLHEEMSLKEALASFNATEAEVLPVFKDQEAGSEFRGVVS
                     RQDALNAYCECTEQA"
     gene            4212..5933
                     /gene="sulP"
                     /locus_tag="PLS90_13855"
     CDS             4212..5933
                     /gene="sulP"
                     /locus_tag="PLS90_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HPK03528.1"
                     /translation="MFVPQLVVCLKDYRRETFFKDLFAGLTVGVIALPLAMAFAIASG
                     LPPERGLYTAIVAGFLISALGGSRVQIGGPTGAFVVIVAGINLQYGYSGLVFCTLLAG
                     GLLIALGLFRMGALIKFIPFPVVTGFTTGIAVVIFSTQIRDLFGLRMEEVPAEFIEKW
                     AAYLSHLGTTNPSALGVGIGTIALIVVLRRCRPRWPGMLIAMAAATVITWALGLEIET
                     IGSRFGDLPRTLPRPGLPRISLAELRELVSPAFTVGLLAAIESLLSATVADGMVGGPR
                     HKSNMELVAQGVANIGSVIFGGIPATGAISRTATSIRSGARTPVAGMVHAVTLLLLLL
                     LFAPLAKLIPLAALAGILVLVSYNMSELHHFVGLLKAPRSDVMVLLSTFGLTVLVDLT
                     VAVQVGVVLAALLFIRRMSEVTNVGMITREFRENGDDAADPNSIWLRDVPPGVEVFEI
                     EGPFFFGAADKFKETMRLIETAPRVIILRMRAAPAIDATGLHALKEFHHQCRNDGIEL
                     VLSGVHAQPLHAMTNDGLLKCIGEENIFGNIDDALNRAREVLGLPPVERPVPFVPTVR
                     REKEQVQ"
BASE COUNT          905 a         1762 c         2398 g         1068 t
ORIGIN      
        1 ggtggccact gcttcttttt gactcacact gtcaggtgaa taccatcaca cttcacgcgt
       61 tgtgctgcaa cgctctgcca gagaatttca gaagagacgg cgtgatgttg ctccagcagg
      121 cgcgacgacg cccaatcagg gggcggcggc agacgatggc gctggaattc aggcgagtgt
      181 gccgtggcgt atttcggcgg acaggtcgaa gcgggcttcg ccgccgaggt cgcgcgggtg
      241 cgtggcggtg atcaacacct gcatcgatcc gcgcagaccg gccaggacgg ccatccgccg
      301 gccggggtca agttcggaac cgaaatcatc gagcatcagg atcggcggtt cgccgagggt
      361 ccgctcgatc cagcgggctg attcgagctt gagcgtgagg gcgacgagcc gatgctggcc
      421 ttgtgaacca aaccgccgca ggtcctgacc cgccaggcgg acggaaaagt cgtcgcggtg
      481 gacgccggcg gcgcagaggc cgtgacgccg tccctcgtcg tagctttcgc gcagacgttc
      541 caggtagatc ccggtcaacg gtcgccacgc gccagagccg gcggccagcg gctcgtccgc
      601 ggaccggggg cgccgggtgt cgaggaacgg ccgggctgtg ccgtcggggt tgatttcata
      661 ccgtcccagg ttggcgtcgt agacgagttc gacgggttcg gcgccgccaa gctcgcgata
      721 gcgcgcggcg aagggctcgg cggcctcggc cagagcggcg gcgcgcgcgc acatcaggtg
      781 cgcccccgcc tcggcgagtt cgggccagta ggcttcggcg agcccgcgca cgtcgcgcgt
      841 cgggggggaa gtgcgcagga cggcgttgac ctgtttgagc gcacgctggt agcgctgaag
      901 ctggccgagg taggcggccg aggtctggct gagcacgata tcgaggaagc ggcggcggtc
      961 ggcggggggg cccttgagca gggcggcgtc ctcgggggtg aaggtgacga cggcggcctc
     1021 gcgccagaga tcgccgaggc gggggagcca ggtcgcgtcg acgaaggcgc gtttccagcg
     1081 gcggccgatc agcaggcgca ggtcgcggcg gccggcgcgg cgctgcaagg cggcgtcgac
     1141 gcgggttggg tcggcggggt cgtcgcggcg gccccagggg aggcagtcct cgtcgcggcg
     1201 ggtgcggaac gagcggccgg tcaggacgca gtgcagggcc tcgaggaggt tggtcttgcc
     1261 ctggccgttg ccgccccaga tcaggttgag gccgggcgcc ggcgcccagc ggagtgtttc
     1321 gatattgcgg aagtgatgca gctcgagcga gcggacatgc atgagggaac cggcgcgggg
     1381 gagtggggag tgacggcggg gcggcagggg ccgccggtcg atacgatagc ccgcggcgcg
     1441 ggggttcgca caggaaaact ggggaggggg acgagggacg aagacgaggg acgagggacc
     1501 gggagggggc atctctattt gcagcggggg gggaatgggg gtataagaga acggtgtgcc
     1561 tatcggccgg cagccgaagg agaggcctcg cgagaggaac caggctgccg ccgcccgctg
     1621 agattctggg agcaacaccg aatttgccag ccgccgcgcc ccgcctcatt gagctgctag
     1681 atcccgcgcg catcatcgat ctggaagtgg agacgaagga agaagccctt cgtcggttga
     1741 gccagttgtt ccagggcgac ccgcgggtgc ttgatgccga ggttttccac caggcgatct
     1801 tgcggcgcga gcagctcgtc agcacagggg tggggtcggg catcgccatc ccgcacgtca
     1861 agatcccgca ggtgacggat tacgtggcgg cggtggggcg gttgcggcgg gggattgatt
     1921 tcgcggcgct cgacaaccag ccggtgcatc tgattttcat gatcggggcg tcggaccgcc
     1981 agacgcgcga gttcgtccgg atcctggcgc aggtgacgca tctgctgaag gacccggcga
     2041 cgcggcagaa gctgctcgat gccgagattc cagacgggtt cctgcaaatc atccagcaac
     2101 atgaacggta gcggagagcc cgtcggcccg agcggcaact cgccggagtc cgtgatggag
     2161 atcacgctcg cgggccggcg gttgcgggtc agccgattca cgtttctgct gctgctgggc
     2221 gtggtggtgg gcgccgtgac gggctgcgtg gccgtgattt tccggctggc ggcgttttac
     2281 ggggagcggt tgctgtttcc ggcgagcgag cattcggcga tccacgtgat tccgtggctg
     2341 ccgccgtggc tgctgcggct gctgatgccc gcggtgggcg gtctggtgag cgggctgatt
     2401 ctgtatcggc tgctgaaata cgccggcggg cacagcatcc cggcgatcct gcaggcggtg
     2461 gcgtcggggc agagttactt caaggcgtcg atggcgtttc cgccgtcgat ggcgatcgtg
     2521 acgatcgcga cgggagggtc ggtggggccg gaggggccga tcgccgagat tggttcggtg
     2581 acgggcagcc tgatcgggcg atgggcgcgg atcccgccgc ggctggtcaa gacgctgatc
     2641 ggagcggggg tggcggcggg gatcgcggcg gtgttcaatt cgccgatcgg gggcgtgttt
     2701 ttcgcgatcg aggttattct gcgcaattac gagattgcgt cgctgacgcc gattctggtg
     2761 gcggcggtgg tttcctcggt gatcgcgcag gcggcgctgg gcgaccggat ggcgattgaa
     2821 tttccgaacc tgggcgacat ccccgccggc gagttgccgt ggttcgccgt gctcgggctg
     2881 ctgtgcggcg cgctgtcgtt cctctacatc cacgggttgg gggcctgcca cgggctgttc
     2941 cggcggtcgg ggatcgcgct gtgggcgcgg ccgatggtgg gcggggtgct ggtcgggctc
     3001 atcgggatcc tgtttccggc ggtgatgggc gagggctacg agtttattcg cggcgtgatt
     3061 cacggcagcg cggcggaagg gagcgtgctg ctgctgctga tgctggtggt gctgaagatc
     3121 gcggcgacgg ggttctcgct cggctcgggg aacccggggg gctcgtttgc accggcggtg
     3181 ttcatcggcg tgatggcggg ggcggcttac ggctacgtgc tgcgcgactt ccggctggtg
     3241 caggacgtgg cgccgtatgc gatgacgggg atggcgggat tgatcgcggg cgggctggga
     3301 gcgccgatca ccgcgatcct gatcacgatc cgccatgggg gcgccgacgg gccggaaatc
     3361 atcctgccgg tgatgacaac cgtcgcgttg tgtttcttcg tgatgcagct ccagcgcaac
     3421 gtgtcggtct atacgctgga gtttctgcgg ctggggatcg acctggaccg ggcgcggcag
     3481 gtcgatccgc tgagtctggt gcgggtgcgg agcgtggtac atacggggga tttcgatgag
     3541 ctgccggcgg acatgccggt gcggcaggcg ctggcgcaat tcaaggagag cgccgcgcgc
     3601 tggttcgtgg tgcgggggga aggggggcag ttcgccggga tcgtctcgct gcacgagatg
     3661 cggctggcga tcgcggagga ggaactggcc gatctgctgg tgctggcgga catcaccgac
     3721 agctttcagc cgcggctgca cgaggagatg tcgctgaagg aggcgctggc gagcttcaac
     3781 gcgacggaag cggaggtgct gccggtgttc aaggatcagg aggcggggag cgagtttcgg
     3841 ggcgttgtct cgcggcagga cgcgctcaac gcctactgcg agtgtacgga gcaggcgtag
     3901 cttcaatcgg gagcgcgatg ggtgggtatt gcgcgcgaag acaagggagc cgtgtgacaa
     3961 attccgccta ttccgagtcc agcctctcac tccatcccga ttctcaatct atcaggtccg
     4021 atgtgccgtg caagagcggc cagtcccccc gaatccgttg acatggggag agtaccgact
     4081 cccaggccaa tgccggttat cgactgattg cccatcccgg agcatcctgt tgatttttac
     4141 ctcaggtggg aggaagatgc cgattgcgtg cagcccgccg cgatccgtcc tggaaaggcc
     4201 cctccccgct cgtgttcgtg cctcagcttg tggtgtgtct gaaagactac cggcgcgaga
     4261 cgtttttcaa agacctcttc gccgggctga ccgtgggcgt aatcgccctg ccgctggcga
     4321 tggcgttcgc catcgcctcg ggcctgccgc ccgagcgcgg gctgtatacg gcgatcgtgg
     4381 ccgggtttct gatctcggcg ctcgggggga gccgggtgca gatcggcggc ccgacgggcg
     4441 ccttcgtcgt catcgtcgcc gggatcaacc tgcaatacgg ctactccggg ctggtttttt
     4501 gcacgctgct ggccgggggg ctgctcatcg cgttggggct gttccgcatg ggcgcgctga
     4561 tcaagttcat cccgttcccg gtcgtgacgg ggttcacgac ggggatcgcg gtcgtcattt
     4621 tctccacgca gatccgcgac ctgttcgggc tgcggatgga ggaggtgccg gccgaattca
     4681 ttgagaaatg ggcggcctac ctgagtcacc tggggacgac caatccgtcg gccctcggcg
     4741 tcgggatcgg cacgatcgcg ctgatcgtcg tgctgcgccg ctgccggccg cgctggccgg
     4801 ggatgctgat cgcgatggcg gcggcaaccg tcatcacctg ggcgctgggg ctggaaatcg
     4861 agacgatcgg cagccggttc ggggatctgc cgcggacgct tccgcggccg ggcctgcccc
     4921 ggatcagcct ggcggagttg cgcgagctcg tctcgcccgc attcacggtc gggctgctgg
     4981 cggcgatcga atcgctgctg tcggccacgg tcgccgacgg catggtgggg gggccgcggc
     5041 acaagtcgaa catggagctg gtggcgcagg gggtggcgaa catcggctcg gtgatttttg
     5101 gcgggattcc ggcgacgggc gccatctcgc ggacggcgac gagcatccgc agcggagcgc
     5161 ggaccccggt cgccgggatg gtgcacgccg tgacgctgct gctgttgttg ctgctgttcg
     5221 cgccgctggc caagctgatc ccgctggcgg cgctggcggg catcctggtg ctggtgagct
     5281 acaacatgag cgagctgcac catttcgtcg ggttgctcaa ggcgccgcgc agcgacgtga
     5341 tggtcctgct gtcgacgttc ggactgacgg tgctggtcga tctgaccgtc gcggtgcagg
     5401 tgggggtggt gctggcggcg ctgctgttca tccggcggat gtcggaggtg accaatgtgg
     5461 gcatgatcac gcgggaattc cgggagaacg gtgacgacgc ggccgatccg aactcgatct
     5521 ggctgcgcga cgtgccgccg ggggtggagg tgttcgagat cgaggggccg ttcttcttcg
     5581 gggccgcgga caagttcaag gagacgatgc ggctgatcga gacggcgccg cgggtgatca
     5641 tcctgcggat gcgggcggcg ccggcgatcg acgcgaccgg gctgcatgcg ctcaaggagt
     5701 tccatcatca gtgccggaac gacgggatcg agctggtgct gtcgggcgtg catgcgcagc
     5761 cgctccacgc gatgacgaac gacgggctgt tgaagtgcat cggcgaggag aacatcttcg
     5821 gcaacatcga cgacgcactc aatcgcgcgc gcgaggtgct cgggctgccg ccggtggagc
     5881 ggccggtccc gttcgtgccg acggtgcgtc gcgaaaagga acaggtgcaa tagagccggg
     5941 tctggcgggc gcggccaatg cggcccggtg gagttcccgt cgagccggcc ggacggcgcg
     6001 ccgggaaacc gcgcgggcgg aagcgggcct tgcccttggc gattcaatgc gattagagtg
     6061 atcgaacagc gtgcgggggc cgcgagcgcc ccgcgcggcc gcaggaagaa cccgcccgag
     6121 cgaatcaacc agg
//
LOCUS       DAOPCO010000244         6126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11285_length_6126_cov_5.134574, whole genome shotgun sequence.
ACCESSION   DAOPCO010000244 DAOPCO010000000
VERSION     DAOPCO010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_6126_cov_5.134574"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1865)
                     /gene="carB"
                     /locus_tag="PLS90_13860"
     CDS             complement(39..1865)
                     /gene="carB"
                     /locus_tag="PLS90_13860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPK03529.1"
                     /translation="MKLSTDLLDLLRRAKQHGFSDQQIAWTINRHLDGSRQVTEADIR
                     RLRREHGIRPVFKSVDTCAGEFEALTPYYYSTYERHNESVRSDRPKIMILGGGPNRIG
                     QGIEFDYCCVQAVFALKAEGYETIMVNSNPETVSTDYDTSDKLYFEPLTVEDVLAICD
                     IEQPDGVIVQLGGQTPLKLCRELEAEGVKIIGTSPDSIDLAEDRDRFGRLLAELGIPQ
                     PENGSGRTLEEVERVVQRIGYPVMVRPSYVLGGRAMQTVWDRDQLVEFTREAFEVAEG
                     HPILIDKFLANATEVDVDALSDGDDVVIAAIMEHIEEAGIHSGDSACVIPPRLLSDHV
                     IATIRRATHQLARALKVKGLMNVQYAVRDEEVYVLEVNPRASRTVPYVSKAIGQPIAQ
                     YAARLMVGRTLREIGFTEEPRIHFYAVKEAVLPFNKFPGCTVILGPEMRSTGEVMGVD
                     ADYAMAFAKSQLAAGSKLPLDGRIFVSVNDYDKRQFLPIARKLVELGFEFLCTAGTHR
                     FLSDNGVASRQVEKLSEGRPNIQDLVMNNEVGMIVNTFSGDKARHDEKMIRTLAIRYE
                     LPLFTTIAAARAVAQAIAALKVKQLEVKSLQEYHAELRARPS"
     gene            2136..2465
                     /gene="cutA"
                     /locus_tag="PLS90_13865"
     CDS             2136..2465
                     /gene="cutA"
                     /locus_tag="PLS90_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPK03530.1"
                     /translation="MSQYMMIVTTTARMQDASRIAERLVEQHLAGCVQIAGPMLSTYR
                     WENEIQRSEEYMVIIKSRADLYEQVETAIKEIHPYEVPEIVGINVEAGSNEYLGWLAG
                     ELRKEQM"
     gene            2968..4422
                     /locus_tag="PLS90_13870"
     CDS             2968..4422
                     /locus_tag="PLS90_13870"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPK03531.1"
                     /translation="MAVQLDTLISLCKRRGFIFQSSEIYGGMAACYDYGPLGVELKRN
                     VRNAWWRAMVQERADVEGIECSILMHPDVWRTSGHVENFTDPLVDCKSCRGRFRADHV
                     ANYRARYRVSENGGTREEELGPEIPSKVFRDAQKIEFLGSPTHCPTCGNAELTAPRLF
                     NLLFKTNVGPIEDASSVVFMRPETAQGMFVNFENVRGTMRRKLPFGIAQIGRSFRNEI
                     TTKAFIFRTLEFEQMEMEFFCKPGTDEEWYRHWVAERFNWYLRYGIRRENLRLRQHDK
                     DELAHYSRMCHDVEYNFPMGWGELEGIAHRGDYDLSAHMKATGKDLQYFDPETNTKYT
                     PYVIEPAAGVDRTVMTFLIDAYAEETVLDRPRTVLRLHPALAPIKVAVLPLLKKRGEI
                     VEICQRLVTAFKQHWPAVYDDTAAIGKLYRRQDEVGTPYCVTVDVDSLEDGKATVRER
                     DTMEQERIELDRLEEYLTERIVLAPPKVEERVEV"
     gene            4503..5096
                     /locus_tag="PLS90_13875"
     CDS             4503..5096
                     /locus_tag="PLS90_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03532.1"
                     /translation="MVRRMRSPERDWDIAWPHAPTHRLSESGTYLVTSGTYLKQHFFR
                     DRERLAVLQRGLLTVARKYGWRLEAWAVFSNHYHFIGHSPANADSAESLSVMLGGLHE
                     KLAKWVNMRDQQPGRQVWHNFWETRLIYERSYLARLHYVHRNPVKHGLVRVAALYPWC
                     SAGWFERTASGAQVKTIYAFKTDQLRVVDDYEPASDW"
     gene            complement(5303..>6126)
                     /locus_tag="PLS90_13880"
     CDS             complement(5303..>6126)
                     /locus_tag="PLS90_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03533.1"
                     /translation="SLAFGWYLAVVFKELSRLDYFLHEEVFNRVFTGEHGRSKSPVFY
                     FWVIPLGLAPWVFLWPALLRRALRRLRRGRGWRGWRAGWNALEPVHRFSALWFAVPLL
                     VFTLSNSKMYLYVVPLMAPVALWAGRLLVERWGAPWPPAGEGSRRARLRYRTVAAAWL
                     ALMAAVRLMPDAAPRTRSHVELSRQILALEPRPDRLYSETSPPNSISFYTGLPITELD
                     LDEEQILPHLHNELAAGRQIHLILKRDDLDDLLKFGWRELAGDDRLALITARELD"
BASE COUNT         1120 a         1698 c         2255 g         1053 t
ORIGIN      
        1 gggggcgagg acgaggacga ggaggaggga gaggggggtc aggagggtcg tgctcgtagt
       61 tcggcgtggt attcctgcag cgacttgacc tcgagttgtt tgaccttgag cgctgcgatg
      121 gcctgcgcga cggcgcgggc ggcggcgatc gtcgtgaaca ggggcagttc atagcggatc
      181 gcgagagtgc ggatcatttt ctcgtcgtgg cgcgccttgt cgccgctgaa cgtgttgacg
      241 atcatcccca cctcgttgtt catgacgaga tcctggatgt tggggcggcc ctcgctcagc
      301 ttttcgacct gccgcgaggc gacgccgttg tcgctgagaa agcgatgcgt ccccgccgtg
      361 cagaggaatt cgaatcccaa ctcgaccagc ttgcgcgcga tcggcaggaa ctgccgctta
      421 tcgtagtcgt tgacgctgac gaagatccgg ccgtcgagcg gcagcttgct gccggcggcc
      481 aactgactct tggcgaacgc catcgcgtaa tccgcgtcca cgcccatgac ctcgccggtg
      541 gagcgcatct cggggccaag gatcacggtg cagccgggga atttgttgaa cggcaggacg
      601 gcttccttga cggcgtagaa atggattctg ggttcctcgg tgaagccgat ctcgcgcagg
      661 gtgcggccca ccatcaggcg cgccgcatac tgggcgatgg gttggccgat ggccttgctg
      721 acatagggga cggtgcgcga ggcgcgcggg ttgacttcga ggacataaac ctcctcgtcg
      781 cgcacggcgt actgcacatt catcaggccc ttgactttca aggcgcgcgc cagttgatgc
      841 gttgcgcggc ggatcgtcgc gatgacgtgg tcggaaagga ggcgtggggg aatgacgcag
      901 gccgaatcgc ccgagtgaat gccggcttcc tcgatatgct ccatgatcgc cgcgatgacg
      961 acgtcgtcgc cgtcgctcag ggcgtcgacg tcgacctcgg tggcattggc caggaacttg
     1021 tcgatcagga tcggatggcc ctcggcgacc tcgaacgcct cgcgggtgaa ctcgacaagc
     1081 tgatcgcggt cccagacggt ctgcatggcg cggccgccga ggacgtaaga ggggcgcacc
     1141 atgacggggt agccgatgcg ctggacgacg cgctcgactt cctccagggt gcgtccggag
     1201 ccgttttcgg gctgggggat ccccagttcg gccaggaggc ggccgaaccg gtcgcggtct
     1261 tcggccaggt cgatcgagtc gggcgaggtg ccgatgatct tgacgccttc ggcctcgagc
     1321 tcgcggcaga gcttgagcgg ggtctgaccg cccaactgca cgatgacgcc gtcgggctgt
     1381 tcgatgtcgc agatggccag gacgtcctcg acggtgagcg gctcgaagta gagtttgtcg
     1441 gaggtgtcgt agtcggtcga gacggtctcg gggtttgaat tgaccataat cgtctcgtag
     1501 ccttcggcct tgagggcgaa gaccgcctgg acgcagcagt agtcgaattc gatgccctgg
     1561 ccgatgcggt tgggaccgcc gccgaggatc atgattttgg ggcgatcgct ccgaaccgac
     1621 tcgttgtggc gctcgtaggt ggagtagtaa tacggggtca aggcctcgaa ctcgccggcg
     1681 caggtatcaa ccgacttgaa gacgggacgg atgccgtgct cgcggcgcag gcggcggatg
     1741 tcggcctcgg tcacctggcg cgagccgtca agatgccggt tgatcgtcca ggcgatctgc
     1801 tgatcggaga atccgtgttg tttggcgcgg cgcagcaggt ccagcaggtc ggtggaaagc
     1861 ttcatgaaat cgagccatcg acaggggagg gtgtcgcccg cgcgggaggg cagggcatgc
     1921 tatcgggccc atcatcgcac aggtcggccc agggccgcaa gattgatggg tcgcggccgg
     1981 tgggaagcga tcggttggga gcgggggaat cgtgggcgag cgttgcggcg ggcgcggcgg
     2041 aagcagccgg cgtgaggcgt gggatgaatt tttcgtgaca ttcggtcggg cttgcgcaaa
     2101 gtgaaggtgg gagtccgagg accggagaga agcccatgag ccagtatatg atgatcgtga
     2161 ccacgacggc gcgaatgcag gacgccagtc ggatcgccga gcgcctggtg gagcagcacc
     2221 tggccggctg cgtgcagatc gcggggccga tgctctcgac ctaccgctgg gagaacgaga
     2281 tccagcgatc ggaggaatac atggtgatca tcaagtcgcg ggccgacctg tatgagcagg
     2341 ttgaaaccgc gatcaaggag atccatccgt acgaagtgcc ggagatcgtg gggatcaacg
     2401 tcgaggcggg gagcaacgaa tacctgggct ggctggcggg cgaactgcgc aaggaacaga
     2461 tgtgacgggg gggcgaccgg ggcggccggg atgaggcgga gagggggttg gccgcgttgc
     2521 cggggaaacc gttgaaacgg ttgagaaagt tgttgcgccg atgggaccgg cgccctcgcc
     2581 ggtctctccc ccgtcggccg gcagccttgc ggcatgtgag gggatgactc gatccggata
     2641 acgataccga aattgataac tctgcggctc cgcgtctctg cgtgtggctg ttgtttctcc
     2701 tggttgcggc ggtgccgaat tcgcaggtgc ctgtcgccct ggttgtggca tcgctgcgtg
     2761 atgcggggcg atcatccgat cacgatcgcg atagcgatag cgataccgat cgcgatagcg
     2821 aaacggtttg cgttcgcccg gattgaaaac gcgcccgcga ctgtgatagc ggtataggat
     2881 tcacgccgcc cgcccgccgc cggggactgg gggcgaatcc gtggaaaggc ttccggcgca
     2941 gcgtcgcgcc ggactcggac ccagcacatg gccgttcaac tcgatacgct gatctcgctg
     3001 tgcaagcggc ggggcttcat ctttcaatcg tccgagatct acgggggaat ggcggcgtgc
     3061 tacgactacg gtccgctggg ggtggaactg aagcgcaacg tgcgcaacgc gtggtggcgc
     3121 gcgatggtgc aggaacgggc cgacgtcgag gggatcgagt gctcgatcct gatgcatccg
     3181 gacgtgtggc ggacgtcggg gcatgttgag aacttcactg atcccctggt ggactgcaaa
     3241 agctgccgcg ggcggttccg ggccgatcac gtcgcgaact accgggcgcg ctaccgggtt
     3301 tcggaaaacg gcggaacgcg cgaggaggag ctggggccgg agatcccatc gaaggttttc
     3361 cgcgacgcgc agaagatcga atttctgggc tcgccgacgc actgcccgac gtgcggcaac
     3421 gccgagctga ccgcgccgcg gttgttcaat ctgctgttca agaccaatgt gggaccgatc
     3481 gaggatgctt cctccgtcgt tttcatgcgg cccgagacgg cgcaggggat gttcgtcaac
     3541 ttcgagaacg tgcgcggcac gatgcggcgc aagctgccgt tcgggatcgc gcagatcggc
     3601 cgcagtttcc gcaacgagat cacgaccaag gcgttcatct tccgcacgct cgagttcgag
     3661 cagatggaga tggagttttt ctgcaagccg gggacggacg aggagtggta tcgccactgg
     3721 gtggccgagc ggttcaactg gtatctgcgc tacgggatcc ggcgcgagaa tctgcggctg
     3781 cgccagcatg acaaggacga gctggcgcac tacagccgga tgtgccacga cgtggagtac
     3841 aacttcccga tggggtgggg cgagctggag gggatcgcac accgggggga ttacgacctg
     3901 agcgcgcaca tgaaagcgac ggggaaggac ctgcaatatt tcgaccccga gacgaacacg
     3961 aagtacacgc cgtatgtgat cgagccggcg gcgggggtgg accggacggt gatgacgttt
     4021 ctgatcgatg cgtatgccga ggagacggtg ctggaccggc cgcggacggt gctgcggctg
     4081 catccggcgc tggcgccgat caaggtggcg gtgctgccgc tgttgaagaa gcggggggag
     4141 atcgtggaga tctgccagcg gctggtgacg gcgttcaagc agcactggcc ggcggtgtat
     4201 gacgacacgg cggcgatcgg caagctgtat cggcgccagg acgaagtggg gacgccgtac
     4261 tgcgtgacgg tcgatgtcga ttcgctggag gacggcaagg cgacggtgcg cgagcgcgac
     4321 acgatggagc aggaacggat cgagctggat cggctggagg agtatttgac ggagcggatc
     4381 gtgctggcgc cgccaaaggt ggaggagcgg gtggaggtgt agggggcagc cagccttttg
     4441 ggagtgcggc ggcagaggac gcgcagcgtc cgccgacgcc gctttggatt cggaagacga
     4501 acatggtccg ccggatgcga tcacccgagc gcgattggga catcgcctgg ccacatgccc
     4561 ccactcaccg gctatcggag agcggaactt atcttgttac gtccgggacc tacctgaagc
     4621 agcatttttt ccgagatcgg gagcgactcg ccgttttaca acgcgggctg ctaacggtcg
     4681 cgcggaaata tggctggcga ctggaggcct gggccgtatt ttcgaatcac tatcatttca
     4741 tcggtcattc acccgcgaac gcagatagcg ccgaaagtct gtccgtgatg cttggcggtc
     4801 tgcatgagaa attggcgaaa tgggtcaaca tgcgggatca gcagccagga cgacaggtat
     4861 ggcataattt ctgggaaacc cgtctgatct atgagcggtc ctatctggcg cgactgcact
     4921 acgtgcatcg aaatccagtg aagcatggac tggtgcgggt ggccgcactt tatccgtggt
     4981 gctcggccgg ctggtttgag cgaacggcct cgggggccca ggtcaagacg atctatgcct
     5041 tcaaaaccga tcagttgagg gtagttgacg attacgaacc ggcttcggac tggtagagga
     5101 tgagagttga taactgcgat ctaaagcggc gtcgacggtc gctccgcgac ctctgccgcc
     5161 gcgctccaaa agggccttgg ccaaagcggc tcgaattgtg gaccccgccc cgggctggtg
     5221 ggcgagcgag gcactgagaa ccgcgatcca aagcgggcgc gcggcggccg cggatgggtg
     5281 acggcggcgg ggagcggggc agttaatcga gttcccgggc ggtgataagg gccaggcggt
     5341 cgtcgccggc gagttcgcgc caaccgaatt tgagcaggtc gtccaggtcg tcgcgtttga
     5401 ggatcaggtg aatctgccgg ccggcggcca gttcgttgtg caggtggggg agaatctgtt
     5461 cctcgtcgag gtcgagttcg gtgatgggca agccggtata gaacgagatc gagttgggcg
     5521 ggctggtctc ggagtagagg cggtcggggc ggggctcgag ggcgaggatc tggcgcgaaa
     5581 gttcgacatg cgagcgggtc cgcggggcgg cgtcgggcat cagccggacc gcggccatca
     5641 gcgccagcca cgcggccgcg acggtgcggt aacgcaggcg ggcgcgccgg ctcccctcgc
     5701 ccgcgggcgg ccacggcgcg ccccagcgct ccaccagcaa ccgaccggcc cacagcgcga
     5761 ccggcgccat cagcggcacg acgtagagat acatctttga attggacagg gtgaagacga
     5821 gaagcggcac ggcgaaccag agcgccgaga agcggtggac gggctcgagg gcgttccagc
     5881 cggcgcgcca gccgcgccag ccgcggccgc ggcggagccg gcgcagggcg cgccgcagca
     5941 gcgccggcca gaggaagacc cacggcgcca gccccagcgg gatgacccag aagtaaaaga
     6001 cgggcgattt ggagcggcca tgctcgccgg tgaagacgcg gttgaagacc tcctcgtgca
     6061 ggaagtaatc gagccggctc agttccttga aaacgaccgc caggtaccag ccgaacgcga
     6121 gcgaca
//
LOCUS       DAOPCO010000245         6038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11487_length_6038_cov_5.136052, whole genome shotgun sequence.
ACCESSION   DAOPCO010000245 DAOPCO010000000
VERSION     DAOPCO010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11487_length_6038_cov_5.136052"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PLS90_13885"
     CDS             complement(<1..1198)
                     /locus_tag="PLS90_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPK03534.1"
                     /translation="MSSRTKLWNRATQPIAALLFVVLIFGGAALLTTTSARAQANLSD
                     TGRYTFSDLAEKVLPSIVTVYVKRDISDQISAREREQFDQFRRLFPGLIPDFPGFPNE
                     GEDGPFMIPSSGSGIIISEDGYILTNAHVIGRKGDNVELRVVLSNDEEIGNEDIQVIE
                     SHELTDLALIKVDRDNLVPIEWGDSDKLRIGERVAAIGSPLDLRATITQGIICANHRE
                     VGPGIVDMIQTDAVINPGSSGGALVNLDGELVGVNRLITTNTGGWQGYGFAIPSNDAR
                     QFVETVQKEGEYASGFIGITMAGAAKNNEQMRRALELDPEMKGVLVEAVRHEEPHPAL
                     DAGLQTGDFIIEADGQPVQDNMDLLKIVARKKVGSRLELKFRRLENGKSREMKTTLTI
                     ARRPAED"
     gene            complement(1482..4067)
                     /locus_tag="PLS90_13890"
     CDS             complement(1482..4067)
                     /locus_tag="PLS90_13890"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPK03535.1"
                     /translation="MAEQPISGTHSEDTPDILTLRIDGLHCANCVRAVERALLALPGV
                     ARAEVDLPSGLARIVPRNGRVPLESLIAAVEQAGYAAVPLSPKEFSAHDAAEAGFACP
                     APGRPAPIEAGQEISLVIAGMDCNSCAAHIERALNALPGVARATVNFATETAHARLHP
                     AHSAAEMLEPLRQAVASAGYTVVGHQTADQGAAAADEARTQRLEARRRRDSEARWWLQ
                     RALEGIGLALPIVALEMGPAAWRAALPHAGWITFALATWIVARVGLPYLRGGWRSLRR
                     GSANMDVLVSMGSGTAYLYSVAVLLAAAAGRPLAMGHTHFHEAALILSIISLGKWLEA
                     RARGQAGHALEGLLDLGARRARVVRDGREVEVPVTEVRVGETLIVRPGEKIPTDGAVL
                     QGESAADESLLTGESAPVEKRIGDEVVGATINLNGMLRIRASRVGEATALAGIIRLVE
                     QAQAGKTRIQRLADQVSAVFVPLVVLVAIATLLGWGLLGGAWTDGILRAVAVLIIACP
                     CALGLATPTAILVGTGQGARHGILIREPQALERARAIQVIVLDKTGTVTEGRPEVTDI
                     VALGAATPEAVLATAAAIEQYSEHPLGQAVTRRARVSLAELPEPTDFKAVSGAGVEAK
                     LEGRRWLIGSSRLMAEERIDWPGEAPSTAERLEAQGKTVIALADAQARQVVGLIALAD
                     RVKPTSREAIDRLRHTEGLDVWMITGDNEATARAVGRQVGLPDDRILAGVRPDGKAEQ
                     IRRLRSGGREVAMVGDGINDAPALAESDLGIAMSSGADLAKEAGTITLMRSDLRDVAK
                     AIALSRAMMRKIRQNLFWAFCYNVVLIPVAALGYVPLLAAAAAMALSDVCVIANALLL
                     KRLRL"
     gene            complement(4329..>6038)
                     /gene="ilvB"
                     /locus_tag="PLS90_13895"
     CDS             complement(4329..>6038)
                     /gene="ilvB"
                     /locus_tag="PLS90_13895"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011814380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPK03536.1"
                     /translation="YDAIFRFNRAAEEKGEPNIRLVVPANEQGAGFMAAGYARASGRV
                     GVFLVTSGPGATNCVTPIRDCMADSVPVVMISGQVPRAAMGTDAFQEAPVFNIMSACA
                     KHVFLVSDPEELEATIRTAFEIARTGRPGPVVIDVPKDVQNWSGTFIGGGLLDFRGYN
                     HRLEQLRRRHVTEEQGRAFFNMLLHSERPLVYAGGGVINSNGAEQLTEFCKTFGIPVV
                     TTLMGIGSIDTADPLALHMLGMHGTASANYAVEDCDFLIAVGSRFDDRVAGKVQEFAP
                     NARCLAHIDIDAAEIGKVKQVTWSFVGDARTGLSDLLELGRRLKFNKDFAPWREHVLK
                     LKEHYRLNFDRDSELIQPQYVIECLNRLTRGEAIITTGVGQHQMWAAQYFDFAHPRHW
                     LTSGSMGTMGFGLPAAIGAQFACPEKMVIDVDGDGSLRMNLGEMETVTTYNLPVKILL
                     LNNLGDGMVRQWQKSYFMNRFSGSDKSLHRKDFVKAAEADGFEFARRLDRKQDTEAVL
                     KAFVEASGPAFLEVMIDPDACVFPMVGPGMSYKEMVTGDWIPKREAPAKPVVSEVEMP
                     DLF"
BASE COUNT          961 a         1863 c         2197 g         1017 t
ORIGIN      
        1 cgtcctccgc gggccgccgc gcaatcgtca gcgtagtctt catctcgcgg gatttgccgt
       61 tctccagccg gcggaacttg agttcgagcc gggagcccac cttcttgcgc gcgacgatct
      121 tgagcaggtc catgttgtcc tggacgggct ggccgtcggc ttcaatgatg aagtcgccgg
      181 tctgcaggcc ggcgtcgagg gcgggatgcg gctcctcatg gcggacggct tcgaccagca
      241 cgcccttcat ctcggggtcc agttcgagcg cccggcgcat ctgctcgttg ttcttcgcgg
      301 cgccggccat ggtgatgccg atgaagccgg aggcatactc gccttccttc tggacggttt
      361 cgacgaactg ccgggcgtca ttggacggaa tggcgaagcc atagccctgc cagccgccgg
      421 tgttggtggt gatgagccgg ttgacgccga cgagttcgcc gtcgaggttg accagcgcgc
      481 cgccgctgga gccggggttg atcacggcat cggtctggat catgtcgaca atgccggggc
      541 cgacttcacg gtgattggcg cagatgatgc cctgggtgat ggtggcgcgc agatcgagcg
      601 gactgccgat ggcggcgacg cgttcgccga tgcgcagctt gtcgctgtcg ccccattcga
      661 tcggaaccag gttgtcgcgg tcgaccttga tcagcgccag atcagtcagt tcgtggctct
      721 cgatcacctg gatgtcctcg ttgccgatct cttcgtcatt ggagaggacg acgcgcagct
      781 cgacattgtc gcccttgcgc ccgatcacgt gggcattggt caggatgtag ccgtcttcgg
      841 agatgatgat tccggacccg gaggagggga tcatgaacgg cccgtcctca ccctcgttgg
      901 ggaagccggg gaagtccggg atcaggccgg ggaagaggcg gcggaactga tcgaactgct
      961 cgcgctcgcg agcggagatc tggtcggaga tatcgcgctt gacgtagacc gtcacgatgg
     1021 acggcaggac cttttcggcc agatcggaga aggtatagcg cccggtgtcg gagaggttgg
     1081 cctgggcgcg ggcggacgtc gtcgtcaaga gggcggcgcc gccgaaaatc aatacgacga
     1141 aaagcagcgc agcgatcggc tgcgtggctc gattccatag cttcgttcgg gaagacatga
     1201 agacctgcct ccatcagcgg attcaaaagg ggagcttcga ggcagcgcct cgtcagcgtc
     1261 gggtcgcgtc gggcggacga ggcccatggc cgcgaaattg aaccagtctc gtaccagttg
     1321 ctcgcatggc gccgccggcg gcgcggcggc gtggaaatcc caacattccg ctcatgatca
     1381 tgcccggttc gggcatccag gtccagtgcg cccctgccgt ggcggcgcgg gagaagcgaa
     1441 cgtgtcctgt gatatgcgac aatttgtcgc tcccgggcgg tctacaaccg cagccgtttc
     1501 agcaacaggg cgttggcgat gacgcagacg tcggacaggg ccatcgcggc ggcggcggcg
     1561 agcagcggga catagcccag cgcggccacg ggaatcaaga cgacattgta gcagaaggcc
     1621 cagaacagat tctggcgaat cttgcgcatc atcgcgcgcg aaagggcgat cgccttggcg
     1681 acgtcgcgca ggtcgctgcg catcagcgtg atggtgccgg cctccttggc cagatcggcg
     1741 ccgctgctca tggcgattcc caggtcggac tcggccagcg cgggggcatc gttgatgccg
     1801 tcgccgacca tggccacctc gcgtccaccc gaccggagcc ggcggatctg ttccgccttg
     1861 ccgtcggggc gcacgccggc caggatccga tcgtcgggga ggccgacctg gcgcccgacg
     1921 gcgcgcgcgg tcgcctcgtt gtcgcccgtg atcatccaga catcgaggcc ttcggtgtgc
     1981 cgcagccggt cgattgcctc gcggctggtc ggtttgacgc gatcggccag cgcgatcaag
     2041 ccgacgacct gccgggcttg ggcgtcggcc agggcgatga cggtcttgcc ttgcgcttcg
     2101 agccgctcgg cggttgacgg ggcttcgccg ggccagtcga tccgctcctc ggccatcagc
     2161 cgactggagc cgatcagcca gcggcgacct tccagcttcg cttcaacccc cgcgccggag
     2221 accgctttga aatccgtcgg ttccgggagt tcggcgaggc tcacccgggc gcggcgcgtc
     2281 acggcctggc cgagcgggtg ctcgctgtac tgctcgatgg cggcggccgt ggccagcacg
     2341 gcttcgggcg tggcggcgcc gagcgccacg atgtcggtca cttccggccg gccttccgtc
     2401 accgtgccgg tcttgtccag cacgatgacc tggatcgcgc gcgcacgctc caaggcttgc
     2461 ggctcgcgga tcaggatgcc gtggcgcgcg ccctgaccgg tgccgacgag gatcgccgtg
     2521 ggcgtggcga gaccgagggc gcaggggcag gcgatgatca ggaccgccac ggcgcgcagg
     2581 atgccgtcgg tccaggcgcc gccaaggagt ccccagccga ggagcgtcgc gatggcgacc
     2641 aggacaacga gcggcacgaa gacggcggag acctggtcgg ccaggcgctg gatccgggtc
     2701 ttgccggcct gggcctgctc gacgaggcgg ataatgccgg ccaaggcggt cgcttcgccg
     2761 acgcgcgagg cgcggatgcg gagcatgccg ttgaggttga tcgtcgcgcc gacgacctca
     2821 tcgccgatgc gtttttccac cggggcggac tcgccggtca ggagcgattc atcggctgcc
     2881 gattcgccct gaaggacggc gccgtcggtc gggatttttt cgcccggccg cacgatcagc
     2941 gtctcgccca cgcgcacctc ggtgacgggc acctcgactt cgcgcccatc gcggacgacg
     3001 cgcgcccggc gggcgcccag gtcgagcaga ccctccaggg cgtgaccggc ctgaccgcgc
     3061 gcccgcgcct cgagccactt gcccagcgag atgatcgaga gaatgagcgc cgcttcatga
     3121 aaatgcgtat gacccatcgc cagcggccgg ccggcggcgg cggccagcag aaccgccacc
     3181 gagtagagat aggccgtgcc cgagcccatc gagacgagga catccatgtt ggcgctgccg
     3241 cggcggagcg aacgccagcc gccgcgcaga taagggaggc ccacgcgcgc gacgatccag
     3301 gtggcgaggg cgaaggtgat ccagccggcg tggggcaggg ccgcgcgcca ggcggcgggg
     3361 cccatttcga gcgcgacgat cggcagggcg agcccgatgc cttcgagcgc gcgctggagc
     3421 caccagcgcg cctcgctgtc gcggcggcgg cgcgcctcca ggcgttgggt gcgcgcctcg
     3481 tcggccgccg cggccccctg atcggcggtt tgatggccga cgaccgtgta gccggcggag
     3541 gcaaccgcct gacgcagcgg ttcgagcatc tcggcggccg agtgggcggg atgcaaccgc
     3601 gcgtgcgccg tctcggtggc gaaattcacg gtcgcgcggg cgacgcccgg caaggcattg
     3661 agcgcgcgtt cgatgtgcgc cgcgcaggaa ttgcagtcca tgcccgcgat gacgagcgaa
     3721 atttcctgac ccgcctcgat cggcgctggc cgaccgggcg ccgggcaggc aaagccggcc
     3781 tccgctgcgt cgtgggcaga gaactccttg ggagacagag gaacggcggc gtagcccgcc
     3841 tgctcgacgg cggcaatcag cgattcaagc gggacgcggc cgttgcgcgg gacgatgcga
     3901 gccaggccgg atggtaagtc gacttcggca cgggcgacgc cggggagagc caagagcgcc
     3961 cgctcgacgg cgcgcacgca attcgcgcag tgcagcccgt cgatcctgag ggtcaggatg
     4021 tcgggagtgt cctcgctgtg cgtgccgctg atcggctgtt cggccatcga tcggattccg
     4081 gttcggtgat ggtcgttccg ccccgggggg acgcgacgat tccattctag gccaatccgg
     4141 tttgattcgc catcggccag agggaaacgg gaggagtggg gacaaaagtg acgggttttc
     4201 gagagtcgag gccgagagac gtatttgagc gagctgagta gaatgtttca tccctcgtcc
     4261 ttctccgtgt gcgatcgtca ttcttgtcga tcgcgaggga cgaaggacga gggactgaag
     4321 ggacggggtt agaacagatc gggcatttcg acttcggaaa cgaccggttt ggccggtgct
     4381 tcgcgcttgg gaatccagtc gcccgtgacc atctccttgt agctcatgcc gggtccgacc
     4441 atcgggaaga cgcaggcatc ggggtcgatc atgacctcga ggaaggcggg accggaggcc
     4501 tcgacgaagg ctttgagcac ggcctcggtg tcctgcttgc ggtcgagccg gcgggcgaac
     4561 tcaaacccgt cggcctcagc ggccttaacg aaatccttgc ggtggagcga tttgtcggag
     4621 ccggagaagc ggttcatgaa gtagctcttc tgccactgac ggaccatgcc gtcgccgagg
     4681 ttgttgagca gcaggatttt gaccggcagg ttgtaggtgg tgacggtttc catttcgccc
     4741 aggttcatcc gcagactgcc gtcgccgtcg acgtcgatga ccatcttctc ggggcaggcg
     4801 aactgggcgc cgatcgcggc gggcaggccg aagcccatcg tgcccatgct gcccgaggtg
     4861 agccagtggc gcggatgggc gaagtcaaaa tactgcgcgg cccacatctg gtgctggccg
     4921 acgccggtgg tgatgatggc ctcgccgcgg gtcaggcggt tgaggcattc gatgacgtat
     4981 tgcggctgga tcagctcgct gtcgcgatcg aagttgaggc gatagtgttc cttgagttta
     5041 agcacgtgct cgcgccaggg ggcgaaatcc ttgttgaact tgagccggcg gcccagttcg
     5101 agcagatcgc tcagtccggt gcgcgcgtcg ccgacgaacg accaggtgac ctgcttgacc
     5161 ttgccaatct cggccgcgtc gatatcgatg tgggcaaggc agcgggcatt gggggcgaac
     5221 tcctggacct tgccggcgac gcgatcgtcg aagcgcgacc cgacggcgat gaggaagtcg
     5281 cagtcctcaa cggcgtagtt ggccgaggcg gtgccgtgca tgccgagcat atggagcgcg
     5341 agcggatcgg cggtatcgat cgagccgatg cccatcagcg tcgtgacgac ggggatgccg
     5401 aaggtcttgc agaattcggt cagttgttcg gcgccgttgc tgttgatgac gccgcccccg
     5461 gcatagacca gcggccgctc gctgtggagg agcatgttga agaaggcgcg gccctgctct
     5521 tcggtcacat ggcgccggcg taactgctcg agccggtgat tgtagccgcg gaaatcgagc
     5581 agtccgccgc cgatgaacgt gccggaccag ttctggacgt ccttggggac gtcgataacg
     5641 accggcccgg gccggccggt gcgggcgatc tcgaacgcgg tgcggatggt ggcttccagc
     5701 tcctcggggt cggagacgag gaagacgtgc ttggcgcagg cgctcatgat gttgaagacg
     5761 ggcgcttcct ggaaggcgtc ggtgcccatc gcggcgcggg ggacctggcc gctgatcatg
     5821 acgacgggga cagagtcggc catgcagtcg cggatggggg tgacgcagtt ggtggcgccg
     5881 gggccggagg tgacgaggaa gacgccgacg cggccgctgg cgcgggcgta gccggcggcc
     5941 atgaagccgg cgccctgttc gttggcgggg acgaccaggc ggatgttggg ctcgcccttt
     6001 tcctcggcgg cgcggttgaa gcggaagatg gcgtcgta
//
LOCUS       DAOPCO010000246         6027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11511_length_6027_cov_3.904220, whole genome shotgun sequence.
ACCESSION   DAOPCO010000246 DAOPCO010000000
VERSION     DAOPCO010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11511_length_6027_cov_3.904220"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1671)
                     /locus_tag="PLS90_13900"
     CDS             complement(145..1671)
                     /locus_tag="PLS90_13900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03537.1"
                     /translation="MAALAALAAILIRLIHLERLGISHWDEGEYVRAARLLADHFTQG
                     LAHLAPEHAPPLVPVLISLSFRLFGPYDFAAILVSAVTGGLTVGLVFWILDRAAGRAA
                     AWGGAIILALCPLHLVYSRMALTDATFTCFHALGLLLSLARIAPAPPSRAFAIRLWLN
                     GLLLGLTTAAAMFTKYQGLIVWLSAACFEALIVAAAALRRRRGAAPASPILLPARAGS
                     LLLALAVAWLCWLPWRLYVLRTYGPTAMHEQHGLFIRPLSDLPQAAFDSSLILLAVLW
                     IWARWAGLLAAVGALPAWRAQRNGTAALLGWGLAYGFAWLLYTPFIRLLTPLLPLLSL
                     LGGVALGALSNAEGSARRAWAGRLMTLLVVGLMALHAAEALRWRSDGYRRLGDRFDEI
                     AALRAGLPLVVRLAHPITFYQRDWPHAMMSDPDAPIDLYGRRFLLLVDTSVDLIPASR
                     RLMDLWRPHAELIWTAENDRLPPTIFNPPARDDIGPMRGAWPIPPHRTHFLLYEVNLD
                     "
     gene            2182..4656
                     /locus_tag="PLS90_13905"
     CDS             2182..4656
                     /locus_tag="PLS90_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK03538.1"
                     /translation="MTEGPDSVGRYHVLSELGRGSMGVVYLGFDPKLQREVAIKTILV
                     PEGYSEAQRQAMTRSLLQEARLAAALAHPGIVRVFDAGEDHSLPYIVFERIEGVALST
                     RLAEEGALGRDGADRLMGELLDALAYAHERQIIHRDIKPANLMVQADGRLRVMDFGIA
                     RALADQPQSGRRVLCGTPRYMSPEQIDGRAVDTRSDVFSLGIVFYEMLCGQRPFPQKD
                     FQELREALVGRPHPPLHHHNPDLPPSYYGFIDRALAKSPEERFADARQMRDALHECMA
                     DRSAPVAAAGEDVSAARQQILDEIRRQIERQGDFPATSQYLARVTAAARSSHSSASTI
                     ANEILKDFALTNRILRMVNSPFYAGIGGRIRTISRAVVILGVDTVLSTAGGLSIFEHF
                     HNRSGVAELKQQAVKALMTGLHARQLAHAINLENAEEAFICGMLHHLGRLIVAFYFPQ
                     EYQRIEKLIDNGTPTERASRKVMRLSYLELGEAMAQFWNMPESIVRAIHGLDPEHKGA
                     PQGSVQMTQAVVSMAEELSHATFITDPEQRQAALKAVERSFRDKIHVGETRLMRLVED
                     SLRNAWDISHALRVDLKSMGVTDALLHSIPADPGAEAAPAARPRPGVSAAAVEATASI
                     SGDVSSPSLTLDDPDDERSQMDENALAVQRQDLMAKTMAEISQALAGQFQLNDVLVMI
                     LEAMYRGIGCDHVLMAMVNPQRSHVIGRFGLGDQIEEAIGRFNFPLRAGGGAPVGCIM
                     NRREYFVPDLSDPAHHGLLPRPLDEVLRAEGLILVPLDVRDQAIGLFMATRRKSRTAF
                     ARQDLRDLRALSRMAVTGIQQSAGQR"
     gene            4831..5322
                     /locus_tag="PLS90_13910"
     CDS             4831..5322
                     /locus_tag="PLS90_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03539.1"
                     /translation="MQSRSFAPSYSRRGFYIIGILIVVAIILILVFKGSMAPDPVTQV
                     SSGITYIDRSQEVACSTNRNAIRTDLQHYQINNPGAPITPDVIRKANSATCPDGGIYL
                     MAQDGHIFCTLHDPPPPEQMQQLMRISEEKPVATPVGTPDPYAGATPQATPMGTPDPY
                     GAR"
     gene            complement(5409..5879)
                     /locus_tag="PLS90_13915"
     CDS             complement(5409..5879)
                     /locus_tag="PLS90_13915"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPK03540.1"
                     /translation="MKRLTQLAICLLIPCLVAGCSWRGFWTGVGLAGAAGAGAAAVYY
                     AKGDLEADLDDDLAHVHKAAHRTLLERGYDVKENELDALEGRLVADIPGLDDEKNRSV
                     KILTERTASALTHISIRVGTFGDEDLSRAILDDIGADLTKSDDAEISDSAAVTH"
BASE COUNT         1099 a         1657 c         2369 g          902 t
ORIGIN      
        1 gggatggatg aacccattgc agaaaaaaat cgtggtctgg ccgatttcga gagccgagga
       61 cggggacgag agccagagcc gaattctggg gtaggccgtt gggggcgatc acgaggacga
      121 gggacgagag acgggggata agggtcagtc gaggttgact tcgtaaagca ggaaatgggt
      181 gcggtgggga gggatgggcc aggcgccgcg catggggccg atgtcgtcgc gtgcgggcgg
      241 attaaagatc gtggggggga ggcggtcgtt ctcggcggtc cagatcagct cggcatgcgg
      301 gcgccagagg tccatcaagc ggcgactggc ggggatcaga tcgacgctgg tatcgacaag
      361 cagcagaaag cggcggccat agagatcgat gggtgcgtcg ggatcggaca tcatcgcatg
      421 gggccagtcg cgctggtaga aggtgatggg atgggcgagg cggacgacga gggggaggcc
      481 ggcgcgtagg gcggcgattt catcgaaccg atcgccgagg cggcgatagc cgtccgaacg
      541 ccagcggagg gcttcggccg catggagcgc catcaggccg acgacgagga gggtcatgag
      601 acggccggcc caggcgcgcc gagcggaccc ttccgcgttc gacagcgcac cgagcgccac
      661 gccgccgaga aggctgagca gcggcagaag gggggtcagc aggcggatga acggcgtgta
      721 gagcagccag gcgaagccgt aggcgagtcc ccagccgagg agagcggcgg ttccgttgcg
      781 ctgcgcgcgc caggcgggga gcgcgccgac ggcggcgagc aggccggccc agcgcgccca
      841 gatccacagg acggcgagga ggatgagcga ggagtcgaag gcggcctggg gcagatcgga
      901 gaggggccgg atgaacaggc catgctgttc gtgcatcgcc gtggggccgt aggtccgcag
      961 gacatacaac cgccagggga gccagcagag ccaggcgacg gcgagggcga ggagcaggct
     1021 gccggcgcgt gcggggagaa ggatcggcga ggcgggcgcg gcgccccgtc ggcggcggag
     1081 ggcggcggcg gcgacgatga gggcctcgaa gcaggcggcc gagagccaga cgatgaggcc
     1141 ctgatatttg gtgaacatgg cggcggcggt cgtcaatccg agcagcaggc cgttgagcca
     1201 gagccggatg gcgaaggcgc gcgagggagg ggcgggggcg atgcgcgcca gcgagagcag
     1261 gaggccgagc gcatggaagc aggtaaaggt ggcgtcggtc agcgccatgc ggctgtagac
     1321 gaggtggagc gggcagaggg cgaggatgat tgcgccgccc caggcggcgg cgcggccggc
     1381 ggcgcgatcg aggatccaga agaccaggcc gacggtgagg ccgccggtga cggccgagac
     1441 gaggatcgcg gcgaaatcgt aggggccgaa gagacggaag ctgagggaga tcagcacggg
     1501 gacgaggggg ggcgcgtgtt cgggcgcgag atgagccaga ccctgggtga aatgatcggc
     1561 gaggaggcgg gcggcgcgga cgtattcgcc ttcgtcccag tgggaaatgc cgaggcgctc
     1621 gaggtggatc aggcggatga ggatggcggc gagcgcggcc agagcggcca taaggaggga
     1681 gtcgctgcgg gcggggcgtt gcatggagcc acagtaaaag agcggggggg gtctgacaag
     1741 gagggggagg ggaagtggct gacatcggtt ggtcgcgccg agggggaaac ggttgaaacg
     1801 gtcaggaatt atttgtagcg gtcggtcacc ggggcgaggc ccggtgcgaa tgagagggcc
     1861 cccgaggggg cccagataag gcccgctggc gcgggctcgt gggacggcgg ctcggggagg
     1921 tgttcgagag ccggggacga gggacgaatt ctgggaggcg gcaagaggcg aggacgaaga
     1981 cgagagacgg cggacgaggg ggagcatatt ccttcaagcc gtctcggggg aagttcgata
     2041 agttctgagg taaggtattc ccgggctgag tcgcccggac ggatccctgc gtgagccctg
     2101 caagctgtca gttccgcctc gtttcgggga aaacgggcgc ttcacgcacc ggtatggctt
     2161 atgcaggcgg ctacttgttc cgtgactgag ggtcccgaca gcgtggggcg ataccacgtc
     2221 ctgtcggagc tgggccgcgg ctcgatggga gtggtgtatc tggggtttga cccgaagctg
     2281 cagcgcgagg tggcgatcaa gacaatcctg gtgccggagg gctacagcga ggcgcagcgg
     2341 caggcgatga cgcgcagcct gctgcaggag gcgcgcctgg ccgcggcgct ggcgcatccg
     2401 gggatcgtcc gcgtgttcga tgcgggggag gatcacagcc tgccctatat tgttttcgag
     2461 cggatcgagg gggtcgccct ttccacgcgg ctggccgaag aaggagcgct gggccgtgac
     2521 ggcgccgacc ggttgatggg ggaactgctc gacgcgctgg cctacgcgca cgagcggcag
     2581 atcattcatc gcgacatcaa gccggcgaac ctgatggttc aggcggacgg gcggctgcgg
     2641 gtgatggact tcggcatcgc gcgggcgctg gccgatcagc cgcagtcggg gcgccgcgtg
     2701 ctgtgcggga cgccgcgcta catgtcgccg gaacagatcg acggccgggc ggtcgacacg
     2761 cggtcggacg tcttttcgct ggggatcgtg ttctacgaga tgctatgcgg ccagcggccc
     2821 tttccgcaga aggacttcca ggagctgcgc gaggcgctgg tggggcggcc gcatccgccg
     2881 ctgcaccacc acaatccgga tctgccgccg tcgtattatg ggttcatcga ccgggcgctg
     2941 gcgaagtcgc ccgaagagcg gttcgccgat gcgcgacaga tgcgcgacgc gctgcacgag
     3001 tgcatggcgg accgttcggc gccggtggcg gcggcgggcg aggatgtctc ggccgcgcgc
     3061 cagcagattc tggatgagat ccggcggcag atcgagcggc agggggattt tccggccaca
     3121 tcgcagtatc tggcgcgggt gaccgccgcg gcccgatcga gccactcgtc ggcgagcacg
     3181 atcgccaacg agatcctgaa ggacttcgcg ctcaccaatc gcattctgcg gatggtgaat
     3241 tcgccgttct atgcggggat cggcgggcgg atccggacga tttcgcgggc cgtcgtgatc
     3301 ctgggcgtgg acaccgtgct gagcacggcc gggggcctga gcattttcga gcatttccac
     3361 aaccgcagcg gcgtggcgga gctcaagcag caggcggtca aggcattgat gaccgggctg
     3421 cacgcgcggc aactggcgca tgcgatcaat ctggagaacg ccgaggaagc cttcatctgc
     3481 gggatgctgc accacctggg gcggctgatc gtggcgtttt attttccgca ggaataccag
     3541 cggatcgaga agctgatcga caacgggacg ccgaccgagc gcgcgtcgcg caaggtgatg
     3601 cggctgtcgt acctggaact tggcgaggcg atggcccaat tctggaatat gccggagtcg
     3661 atcgtgcggg cgatccacgg gctggacccc gaacacaagg gggcgccgca gggctcggtg
     3721 cagatgacgc aggcggtcgt gagcatggcc gaggaactga gtcacgcgac gttcatcacg
     3781 gatcccgagc agcggcaggc ggcgctgaag gcggtcgagc gcagtttccg ggacaagatt
     3841 catgtggggg aaacgcggct gatgcggctg gttgaggatt cgctgcgcaa tgcgtgggat
     3901 atctcgcatg ccctgcgggt cgatctgaag tcgatgggcg tgaccgatgc gctgctgcac
     3961 tcgatcccgg ccgatccggg cgccgaggcg gcgccggccg cgcgcccgcg tccgggggtg
     4021 agcgcggccg cggttgaggc gaccgcgtcg atttcaggcg acgtcagcag tccgtcgctg
     4081 acgctggacg atccggacga cgagcgcagc caaatggacg agaatgcgct ggcggtgcag
     4141 cgccaggatc tgatggccaa gacaatggcc gagatctcgc aggcgctggc gggccagttc
     4201 cagctgaacg atgtgctggt gatgatcctg gaagcgatgt atcgggggat cggctgcgat
     4261 catgtgctga tggcgatggt caatccgcag cggtcgcacg tgatcggacg gttcgggctg
     4321 ggggatcaga tcgaggaggc gatcgggcga tttaattttc cgttgcgcgc ggggggcggg
     4381 gcgccggtcg gctgcatcat gaaccggcgg gagtatttcg tgccggacct gtccgacccg
     4441 gcgcatcacg ggctgctgcc gcgtccgctg gacgaggtgc tgcgggccga ggggctgatt
     4501 ctggtgccgc tggacgtgcg ggatcaggcg atcgggctgt tcatggcgac gcggcggaag
     4561 agccgcacgg cgttcgcgcg ccaggatctg cgcgacctgc gggcgctgag ccggatggcc
     4621 gtgaccggga ttcagcagtc ggccggacag cggtgaaggg gaaagcaaga ttgaattccg
     4681 aaagagcatg agcgcatacc gacagcgatt gcgataccga ttagaaatcc cgatttgttg
     4741 tgccggcctc gaggcttagg gtggcgcggg gaggggggat gtgctaaacg tgaatcaggc
     4801 acgaaaaggc atggaaggcg gcatgaagcg atgcaatccc ggtcctttgc tcccagctat
     4861 tcacggcgcg gattctacat catcgggatc ctgatcgtcg tggcgatcat tctgatcctg
     4921 gtgttcaagg gatcgatggc gcccgatccg gtgacgcagg tctccagcgg gatcacctac
     4981 attgatcgct cgcaggaggt ggcgtgttcg accaaccgca acgcgatccg gaccgatctg
     5041 cagcattatc agatcaacaa ccccggggcg ccgatcacgc cggacgtgat tcgcaaggcg
     5101 aacagcgcca cgtgtcccga cggggggatt tatctgatgg cgcaggacgg acacattttc
     5161 tgcacgctgc acgacccgcc cccgcccgaa cagatgcaac agctgatgcg gatcagcgag
     5221 gagaaaccgg tcgcgacacc ggtggggacg cccgacccgt atgccggcgc gacgccgcag
     5281 gcgacgccga tggggacgcc ggatccctat ggggcgcggt gaggcgggag gctggattcg
     5341 tggcgcgcgc ccggcagcgt tcggccaagc gaccgcgatt gggggaatga gcagcgctgg
     5401 attgcctgct agtgggttac ggcggccgaa tccgagattt ccgcgtcgtc ggatttcgtc
     5461 agatccgcgc caatgtcgtc gaggatcgcg cgcgagaggt cctcgtcgcc gaacgtgccg
     5521 acgcggatgg agatatgcgt gagggcggac gcggtccgtt cggtcaggat cttaacggag
     5581 cggtttttct cgtcgtcgag gccggggatg tcggccacga ggcggccctc gagcgcatcc
     5641 agttcgtttt cctttacatc gtagccgcgc tcgagcaggg tgcggtgcgc ggccttgtga
     5701 acgtgggcga gatcgtcgtc cagatccgcc tcaaggtcgc ccttggcgta gtagacggcg
     5761 gcggcgccgg cgccggccgc gcccgccagc ccgacgccgg tccagaatcc gcgccaggag
     5821 caacccgcca ccaggcaggg gatcagcagg cagatcgcca gttgagtcaa gcgcttcatg
     5881 cttcgtcctc cgaaacagat tgcagagcaa ggacgtcttg attagcacaa cgacagggaa
     5941 aggtcgaggg gcagggtgcg gggggatgag gctccttgat ccttcacggc aaattgaccg
     6001 atgcccctcc atcgcggagc aaacacg
//
LOCUS       DAOPCO010000247         6002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11563_length_6002_cov_3.889860, whole genome shotgun sequence.
ACCESSION   DAOPCO010000247 DAOPCO010000000
VERSION     DAOPCO010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11563_length_6002_cov_3.889860"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="mgtE"
                     /locus_tag="PLS90_13920"
     CDS             complement(<1..1144)
                     /gene="mgtE"
                     /locus_tag="PLS90_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPK03541.1"
                     /translation="MLGKAIQPEIEELIAAGQFDELRDILANNLEAADIAEILDEVND
                     EDRALLFRLLPTQLASDVFSLLEIEGQEKLIQSLSREQVTHILNEMSPDDRTRLLEEV
                     PDEVAQRLLRALNPKELAVARTLLGYPEESIGRRMTPEYVAANPNWTVRQTLDFIRRE
                     APRVETINVLYVLDDQGRLVDDLRLGELLLADPDATIRDICDFQFVSLSAFEDQEEAV
                     EAFKKYDRLALPVVGSKGVMVGIVTVDDVLDLAEEEGTEDVQKFGGQEALEDSYFATT
                     VLQLARKRGLWLTVLLVSSFLTVTAMNHFSYLTVLMPVLTVFIPMIISSGGNSGSQSA
                     ALIIRGLAVREIELANWWRVFLRELAAGLLLGLFLGVIAGGFSVALR"
     gene            1570..2238
                     /gene="pal"
                     /locus_tag="PLS90_13925"
     CDS             1570..2238
                     /gene="pal"
                     /locus_tag="PLS90_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPK03542.1"
                     /translation="MLRCSHSLIRSLAARSLLLGLGLALAVGAGGCSRTKPKKHWWQF
                     WRAPSTELTAEMQHPDSQFIPPAPDVLDPEGGPGSRSIGPDSGELPPPPVAEGTLAEP
                     EARREQPAGAVSGLQTVYFAYDSAELSAEARAILDQNAQWLKANPGYEIQIGGHTDER
                     GTVEYNLNLGDRRAKAVKEYLVSRGIDPNRMHTISYGEEMPIAQGATEEAYSLNRRAQ
                     FLVY"
     gene            2308..3183
                     /locus_tag="PLS90_13930"
     CDS             2308..3183
                     /locus_tag="PLS90_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03543.1"
                     /translation="MNSPQIHRVLRAAATVPLALLLILAVGGCAGLDMGGQQQLMKDI
                     ASLERKIDALADQQSESEHKIAAQLGSVTNNLDQRGGVTLNAVEQLSDRMLAVEQALD
                     EIEASIERLASELESQARSQRNLSQLNPSSRIEEPRPRETTPAENPVSPIESSPVTAQ
                     PSVPSGDIETLVTEAQHQINMGNHDQGIEILEQAMEQSPTKDQQIEIHYWLGEAHFLT
                     NRLPDAFGHYNDLIHLDPRSARAWNSLERMAEIRVNQDRCEDAIKLLDNFKQYPNYDG
                     IDRVNRLRERAEACR"
     gene            3204..3719
                     /locus_tag="PLS90_13935"
     CDS             3204..3719
                     /locus_tag="PLS90_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HPK03544.1"
                     /translation="MARHVAVLVEQSYQELEVWYPIYRLREAGVSVTVVGTGSSPEYP
                     SKLGYPCKADVAIDQVDAADFDGVIIPGGWAPDFLRRYPRINQFVADLNRAGKVVAAI
                     CHGGWVPCSADILKGKRATSFFAIKDDMIHAGCQWVDEETVVDGNLITARKPDDLPAF
                     MKAVLSALGVS"
     gene            3946..5250
                     /locus_tag="PLS90_13940"
     CDS             3946..5250
                     /locus_tag="PLS90_13940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPK03545.1"
                     /translation="MSTIGTTPPTPDTETPKYDPEGFFTPATIRHNMKVLILLEAILM
                     TGSQDLMVALQPLLDYLTESYKLIGFVLGLTWTGVLGAILIPYISRRFAYKKWLYITV
                     SVLWALPLLILGTALVAAPLYNPTTRMLLILTVVLMFIFQMLAGFTYIPHNEFLASCI
                     PPSHRGRMYGISSTLYAVLSLGAIALGGYLIKVLDEPQSFGYVFLMTAGIWLVGYALA
                     TSAREKRTPVENSPKPWTGAMFRELLRTRGYIRFLIFSIVYNMTVTPIFVLLPNYGLK
                     ELNMPPHAVAIIQGIGQAVIIITSAPSGWLIDRLTPKKVVPVSLIITGVGFLICFFLF
                     NAWSIYVSYAVIRIAMAAINPSTTILAVSIPKPEHRAGLFAVQSLIGMLSYGIGPMVA
                     GAISDIIAPRTILLIIGVFSIAFCFATKSLFRPLGDRPQDYY"
     gene            5265..>6002
                     /locus_tag="PLS90_13945"
     CDS             5265..>6002
                     /locus_tag="PLS90_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPK03546.1"
                     /translation="MLHLPVRYLRLYSRQQRYGCSESDCGMVDDTLPLPLRETAFILV
                     DCWNMHYLRSFEERADRICRERIAPALAAARAAGLTVIHAPSPMIAEKYRQAEVYRDP
                     ADQTFAAQFGAPDPDWPPAEFIARAGRHARFARDYAVPEQFWIEPYQKIDIHPAVRPA
                     GDDFVVVNQPQLQRLLKDKRLLHLVYVGFATNMCLMGRDFAVRPMAWLGYNTIVLRDC
                     TTAVEFDASLAELTATRMALAEMEHFYG"
BASE COUNT         1061 a         2210 c         1659 g         1072 t
ORIGIN      
        1 cgcgcagcgc cacgctgaac ccgccggcga tcacgcccag aaacaacccc agcagcaggc
       61 ccgccgccag ctcgcgcaga aacacccgcc accagttggc cagctcgatc tcgcgcacgg
      121 ccagcccgcg gatgatcaac gccgccgatt gcgacccgga gttgcccccc gacgagatga
      181 tcatcgggat gaacaccgtc agcaccggca tcagcaccgt cagatacgaa aagtgattca
      241 tcgccgtcac ggtcaggaaa ctgctgacga gcaggaccgt cagccacagc ccccgcttgc
      301 gcgccagttg cagcaccgtg gtggcgaaat aggaatcctc cagcgcctcc tgcccgccga
      361 atttctggac gtcctcggtc ccttcctctt cggccaggtc cagcacgtca tcgaccgtca
      421 cgatccccac catcacgccc ttgctgccga cgaccggcaa cgccaggcga tcatatttct
      481 tgaacgcctc gaccgcctcc tcctgatcct cgaacgccga gagcgagacg aattgaaaat
      541 cgcagatatc gcgtatcgtc gcatcggggt cggccagcag caactcgccc agccgcaggt
      601 cgtcgaccag ccgcccctgg tcgtccagga cgtagagcac gttgatcgtc tcgacgcgcg
      661 gcgcctcgcg gcggatgaaa tccagcgtct ggcgaaccgt ccagttgggg ttggccgcca
      721 catactccgg cgtcatccgt cggccgatgc tctcctcggg gtagcccagc agtgtgcgcg
      781 cgaccgccag ctccttcgga ttgagcgccc gcagcagtcg ctgggcgact tcgtcgggca
      841 cctcctccag cagccgcgtg cggtcgtcgg gcgacatctc gttgagaata tgcgtcacct
      901 gttcgcgcga gaggctctgg atcagcttct cctgcccctc gatctcgagc agcgaaaaga
      961 cgtccgaggc cagttgcgtc ggcaacagcc ggaagagcag cgcgcgatcc tcgtcgttga
     1021 cctcgtccag gatctcggcg atgtcggccg cttcgaggtt gttggccagg atgtcgcgca
     1081 gttcgtcgaa ctggccggcg gcaatcaact cctcgatttc gggttggatg gcttttccca
     1141 gcatcgatcg gccttcgcgc gtgcgcaggg tagaggaatt ccccccctac cataccggtc
     1201 gccccccgct ttcgccagtc cggccgacgt aaaaattcgc cgcgcgccgt cggttcccct
     1261 cccttgctct ccgtccctga cccaaaagtc cgtccctcgt cctcgtgctt gctgccatcg
     1321 gcctaccccc atactcggat cgcgtccttc atccccggct cttgaagccc tttgccgctc
     1381 ccctcgcctc cccgccaaac aatcgccccg gctcgcgtgt catgtttgcg ctggaaactt
     1441 gggaccgctt gccctagatt ttcaattgat ccgccccgca aggggcccgt ttgaccttgg
     1501 ggctcgcgcc tcagtcaaca ctcccgctct ggcggctgcc gcactccccc gctccggagg
     1561 ttcacgccga tgctacgctg ctcccattct ctcatccgct cgctggcggc ccggtcgctt
     1621 ctgctcgggc tcgggctggc gctcgccgtc ggcgccggcg gctgttcgcg caccaaaccc
     1681 aagaaacact ggtggcaatt ctggcgggcg ccctcgaccg aattgaccgc cgaaatgcag
     1741 caccccgatt cccagttcat tcccccggcg cccgacgtgc tggatcccga aggcgggccc
     1801 ggctcgcgct cgatcggccc cgattccggc gaactccccc cgccccccgt cgccgaggga
     1861 acgctcgcgg aacccgaagc gcgccgcgag cagccggccg gcgccgtcag cggcctgcaa
     1921 acggtttact tcgcctacga cagcgccgag ctgtcggccg aggcgcgggc catcctcgat
     1981 cagaacgcgc agtggctcaa ggccaatccc ggctacgaaa tccagatcgg cggccacacc
     2041 gatgaacggg gcaccgtcga atacaacctc aatctgggcg atcggcgcgc caaggcggtc
     2101 aaggaatacc tcgtctcgcg cggcatcgac cccaaccgta tgcatacgat ctcctacggc
     2161 gaagaaatgc ccatcgccca gggtgccacc gaggaagcct acagcctgaa ccgccgcgcg
     2221 cagttcctcg tctattgatc cgcgatccgg ttttggggat gatgggtgtt cggcttcgat
     2281 gaatccttgg aagggcaatg gctgaccatg aattctcctc agatccaccg cgtgctgcga
     2341 gccgccgcga ccgttccgct cgccctgctg ctgatcctgg ccgtgggcgg ctgcgccggc
     2401 ctcgatatgg gcggccagca gcagctcatg aaggacatcg ccagcctgga gcgcaagatc
     2461 gatgccctcg ccgatcagca gtcggaatcc gaacataaaa tcgccgccca actgggcagc
     2521 gtgaccaaca acctggacca gcgcggcggc gtcacgctga acgccgtcga gcaactgagc
     2581 gaccgcatgc tcgccgtcga gcaggcgctc gatgaaatcg aagcctcgat cgagcggctc
     2641 gcctccgagc tggaaagcca ggcccgctcg cagcgcaatc tctcccagct caacccttcc
     2701 agccgcatcg aggagccccg cccccgcgaa acgacgcccg ccgagaatcc cgtcagcccg
     2761 atcgagtcct cgcccgtgac cgcgcaaccc tccgttccct ccggcgatat cgaaaccctc
     2821 gtcaccgaag cccagcatca gatcaacatg ggcaatcacg accaggggat cgagatcctg
     2881 gaacaggcca tggagcagag cccgaccaag gaccagcaga tcgaaatcca ctactggctg
     2941 ggcgaggcgc acttcctgac caaccgcctg ccggacgcct tcggccacta caacgacctg
     3001 attcacctcg atccgcgctc cgcccgcgcc tggaacagcc tggagcgcat ggcggaaatc
     3061 cgcgtcaatc aggatcgctg tgaagatgcc atcaagctgc tggacaactt caagcaatat
     3121 cccaactacg acggcatcga ccgcgtgaac cggctgcgcg agcgcgccga agcctgccgc
     3181 tgaggcgcaa cgggagtggg ctgatggcca ggcacgttgc cgttctcgtc gagcagagct
     3241 atcaggaact cgaggtctgg tacccgattt accgcctgcg cgaagccggg gtcagcgtca
     3301 ccgtcgtcgg caccggcagc agcccggagt accccagcaa actgggctac ccgtgcaagg
     3361 ccgacgtcgc catcgatcag gtcgacgccg ccgactttga cggggtgatc atccccggcg
     3421 gctgggcccc cgatttcctc cgccgctatc ccagaatcaa tcaattcgtg gccgacctga
     3481 accgggcggg caaggtcgtc gcggccatct gccacggcgg ctgggttccc tgctcggccg
     3541 atatcctgaa gggaaaacgc gcgaccagtt tcttcgccat caaggacgac atgattcacg
     3601 ccggctgcca gtgggtggac gaggaaacgg tggtcgacgg caacctgatc accgcccgca
     3661 aacccgatga cctgcccgcc ttcatgaaag cggtcttgtc agccctgggc gtctcctgac
     3721 cacccctccc gcaggaattg cggaccgcgt cctcgttctc ggaatcgccc cgaacggccc
     3781 tcccaagaga aattcgggcc ccgcgtcact gcgcttccgc ctgatcccat tcgctatccc
     3841 ctcccccccg cccaactttc accttccgtt tacaatccgg taaacctata gttacagact
     3901 cttcccgcat gacattccac gccgccccgc gcgaccggga gcaccatgag caccatcgga
     3961 accaccccgc cgacgccgga taccgaaacg ccgaaatacg atccggaggg cttcttcacc
     4021 ccggcaacca tccgccacaa catgaaggtc ctgatcctgc tcgaggcgat cctgatgacc
     4081 ggctcgcagg atctgatggt cgcgctccag ccgctgctgg actacctgac cgaatcctac
     4141 aagctgatcg gcttcgtcct gggcctgacg tggaccggcg tcctcggcgc gatcctgatt
     4201 ccctacatct cccgccgctt tgcctataaa aagtggctgt atatcacggt ctcggtgctc
     4261 tgggccttgc ccttgctgat cctgggcacg gcgctggtcg ccgccccgct ctacaacccc
     4321 accacgcgca tgctgctgat cctcaccgtc gtcctgatgt tcattttcca gatgctcgcc
     4381 ggattcacct acattccgca caatgaattc ctcgcctcgt gcattcctcc cagccatcgc
     4441 ggccgcatgt acggtatctc cagtacgctt tacgcggtcc tctcgctcgg tgcgatcgcg
     4501 ctgggcggct atctgatcaa ggtgctcgac gagccgcagt cgttcggcta cgtcttcctg
     4561 atgacggccg gcatctggct cgtcggctac gcgctggcga cttcggctcg cgaaaagcgc
     4621 acgccggtcg aaaactcacc caagccctgg accggcgcga tgtttcgcga actcctgcgc
     4681 acgcgtggct acatccgttt cctgatcttc tccatcgtct acaacatgac ggtcacgccg
     4741 atcttcgtcc tgctgcccaa ctacggcctc aaggaactga acatgccgcc ccatgcggtg
     4801 gcgatcatcc agggcatcgg ccaggccgtg atcatcatca cctcggctcc gtccggctgg
     4861 ctgatcgacc ggctcacacc caagaaggtc gtccccgtct cgctcatcat caccggcgtc
     4921 ggcttcctga tctgcttctt cctgttcaac gcctggagca tctatgtcag ctatgccgtc
     4981 atccgcatcg cgatggccgc gatcaacccc tccaccacga tcctggccgt cagcatcccc
     5041 aagccggagc atcgcgccgg cctgttcgcg gtgcagtcgc tgatcggcat gctcagctat
     5101 ggcatcggtc cgatggtggc cggggccatc agcgatatta tcgcgccgcg cacgattctg
     5161 ctgatcatcg gcgtcttttc gatcgccttc tgttttgcca ccaaaagcct gttccgcccg
     5221 ctgggcgacc gcccgcagga ttactattga cccgaggccg cgccatgctc cacctacccg
     5281 tccgctactt gagactctac agccggcagc agcgctatgg ctgcagcgaa tcggactgcg
     5341 gcatggtcga cgacacgctc cccttgcccc tccgcgagac ggccttcatc ctcgtcgatt
     5401 gctggaatat gcattacctg cgctcatttg aagagcgcgc cgaccggatc tgccgcgaac
     5461 ggatcgcccc ggcgctggcc gcggcccgcg ccgccgggct gaccgtcatc catgcccctt
     5521 cgcccatgat cgccgaaaaa tacaggcagg cggaggtcta ccgcgacccc gccgaccaga
     5581 ccttcgcggc gcaattcggc gcccctgatc ccgactggcc cccggcggaa ttcatcgcgc
     5641 gcgccggccg ccatgcccgc ttcgcccgcg attacgccgt ccccgagcag ttctggatcg
     5701 aaccctacca gaagatcgat atccatccgg cggtccggcc ggccggcgac gacttcgtcg
     5761 tcgtcaacca gccgcaactc cagcggctgc tcaaggacaa gcgcctgctc cacctggttt
     5821 acgtcggctt cgccaccaat atgtgtctga tgggtcgcga tttcgccgtc cgcccgatgg
     5881 cctggctcgg ctataacacg atcgtcctgc gcgattgcac caccgccgtc gaattcgacg
     5941 ccagcttggc cgagctgacc gccacgcgca tggcgctggc cgaaatggag cacttctacg
     6001 gc
//
LOCUS       DAOPCO010000248         5982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11606_length_5982_cov_5.293909, whole genome shotgun sequence.
ACCESSION   DAOPCO010000248 DAOPCO010000000
VERSION     DAOPCO010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11606_length_5982_cov_5.293909"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..5902
                     /locus_tag="PLS90_13950"
     CDS             140..5902
                     /locus_tag="PLS90_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03547.1"
                     /translation="MSWGAPASATHAAESLRLRSEFPRSGGDFGAAVACIPDVDGDGA
                     PDIAIGAPMEGPGSLPDGAGMVYVYSGADAALLYYLAPRPEAGAGRFGFAVAGSPDLD
                     GDGRGEIIVGAPRQRIASSSETGRVYLFSGATGILIREFVPESSYAGDPCWFGHAVGS
                     VADADQDGQPEILVGSPWERAAASYSSLHGGVVHLFSGASGQRIGRFVPPEFGFDIFY
                     QFGYSVAGAADITGDGRGDALCGAPLAWCGGGGTQGGRAYLYAGGLNSLARLYESPSP
                     ASGGYFGAAVAAVRDFTLDGHADVAVGAPGEGGGSLPAGAGRVYLFNARGGSSAASEP
                     PWRTLTSPNAEAGGRFGEALAAIAGGGDLLVGAPGETSRGVARAGRVYVYDVLKSGPT
                     RIFEPPVVATGSGFGGALSGALDFEGDGQFDLLAGLPGVTPAGTALASGETVLLKSSD
                     GYATPALTLPPEPTPTPTPLPLVPEKTDLLSSTSLPFGLVTRILGDDEQDNLGESIAF
                     GDFDGDGYDDLAVSSLFGGLGEGFGWARGDVVVLFGRRDPPLVVDLNTGGAARAGESR
                     LGGEMDFGLFGFALAAGDINGDGYDDLVIGAHGESPGGRSGAGTVYVVYGRTDAAGRV
                     VSVNPDGAPAQGATCILGAAEEDWLGSALACADIDGDGDDDLILVAGGHDGPERPDAG
                     AVYRIPGGADLPGQRIDLADPPGHYGETVILGVRETDFLGNAVAAADLDGDGRGEIII
                     GAPGLEYAFRPNAGGVWVIPADAGEAVGGGRRVDLAAPAAAWGITLIAGRAGDQLGTS
                     LATGDVNGDGRADLIVSAPTADRDGKALAGEAYIFPGGEASPHGWAGGVVDLRRGVGA
                     QGETVIYSMLARDTLGYRVAAGDVDGDGIDDILYSRAGMEQPAPDTRGVMEILLGRRG
                     GIGAAAGSGRIVAGSDLRVAGEQVNSSFAYALGAGGDMNRNGLPEWAVSAPLGDNPGV
                     AGFADNNAGRAYALGDLAGTESARAAAVHRAGDAPIRGIGGRLTPALRCLVGYQGGDA
                     ASITTATLVRTPAARAEAHPPPGAAPGLWHLETDREGWTSARVAFDFTDGELAGLDPA
                     QLRLLRAPSAAGPWVPVAEAAFDARRLRLSATVEALGWFCFGPDLLTARVEQGANQTD
                     PAHGVPIVFDVTFSAPVDDFAAEDVTFAGSAKVLEYSLSGAAASYGLAVHRATAGEII
                     PVIAAGVAHDGDGRANQAAVGIDACVTYQPAPDVALRVGGVALENGETYAAPETLQGS
                     PDARLEIELENSGEEVLRVWGVALAPPFRLLGEPPETVDPGQAARMTAAIDTGVRGTF
                     TAAFGFETNDPNESRVTVQLEARVVGPDLTVAREPIDFGLWDQDGTLVDDVWLAVVNS
                     GERTLEIPETRLIGPDADAFVAEPPPTPIGSGRQSAIRVRYAPRDLGAHEAVLCILSN
                     DYDTTEVLIAVRGETTSRPDLRVGFSPPDTPLEPGVDFELGFSYGNARPRTATGVALR
                     LELADGLQLIPQSGGEPWVQEGEGRYLLNLKSLGQGTIHSRLVVRSAERIPAGVESYR
                     VRLSIEDDGLNGADSRTGNNAATVDLPVAAQPDLTLRMDMLTTRVLAGYPLVVSLCAG
                     NRGNQDATNVEVRVELPEPMTFREDLSTPGWRPAGGRGLEFALAELASATSRTLELGL
                     ELALAAPRASAFVTLAAMATDDGANGADPVPSDNAAEARIPCAWGMGGLALVANTPSA
                     AWAVAGPLDFDRSGTGSATLECVAGEYAVRWHSNPYWAAPEGGPVAATVDVDQVTTVA
                     ACYERLRGSIHVRVLPGETRWRLDGEGTSFEGEGERLLTDLPAGIHAFRPAPPAGYLK
                     PYESRPVAVPGGARVNLLEIFMPAEAGPEQVEARLLRCLLGLDSPAEDLDLNGDGLID
                     AGDLIQCVREQDPAAPAMRR"
BASE COUNT          792 a         1794 c         2480 g          916 t
ORIGIN      
        1 tgatcgctgc tccattgtcc tgcacaactc ccaatcgtcg cgtcaggagt tgtcctggtt
       61 tatgccagtc cgttgggccg cgggtctcgg gcgccgtgtt tcggttccgg accgctggat
      121 tcaattcctt ttgctgcttg tgagctgggg cgcgccggcg agtgcgaccc acgcggccga
      181 gtcgctgcgc ctgcggtcgg agtttccgcg cagcggcggg gatttcggcg cggcggtggc
      241 ctgcattccc gacgtcgacg gggacggggc gcccgacatt gcaatcggcg cgccgatgga
      301 agggcccggg tcgctgccgg acggcgcggg gatggtttac gtctattccg gggcggacgc
      361 tgcgctgctg tattatctgg cgccgcggcc ggaggcgggg gcgggccgat tcgggttcgc
      421 ggtggccggc tcgcccgatc tggacggcga cggccgcggg gagatcatcg tcggcgcgcc
      481 ccgccagcgg atcgcgtcga gcagcgagac cggccgcgtc taccttttct cgggcgcgac
      541 ggggatcttg atccgggagt tcgtgccgga gagcagctac gccggcgatc cctgttggtt
      601 cggtcatgcg gttggcagcg tcgccgacgc cgaccaggac gggcagccgg agatcctggt
      661 cggttcgccg tgggagcggg ccgccgcttc gtattcgagc cttcacggcg gggtggtgca
      721 tctgttttcg ggggcgagcg gccagcggat cgggcggttc gtcccgccgg agttcggttt
      781 cgacattttc tatcagttcg gttactcggt ggcgggggcg gccgacatca cgggagacgg
      841 ccgcggcgat gcgctgtgcg gggcgccgct cgcgtggtgc ggcggcggcg ggacgcaggg
      901 gggacgcgcc tacctgtatg cgggcgggtt gaacagtttg gcgcgactgt atgaatcgcc
      961 cagcccggcg tccggcgggt atttcggcgc ggcggtggcg gcggtgcgcg acttcacgct
     1021 ggacggccat gccgacgtgg ccgtcggcgc gccgggggaa gggggcggat cgctgcccgc
     1081 cggcgcgggg cgggtctacc tgttcaatgc gcgcgggggg agcagcgcgg cgagcgaacc
     1141 gccgtggcgc acgctgacgt cgcccaatgc ggaagccggc gggcggttcg gcgaggcgct
     1201 ggccgcgatc gcggggggcg gcgacctgct ggtgggcgcg ccgggcgaga cgagccgcgg
     1261 cgtcgcgcgc gccggccgcg tgtatgtcta tgacgtcctg aagagcgggc cgacgcggat
     1321 ttttgagccg ccggttgttg cgaccgggag cgggttcggc ggcgcactga gcggcgcgct
     1381 cgatttcgag ggcgacggcc agttcgacct gctcgccggg ctgcccggcg tgacgccggc
     1441 ggggacggcg ctggcctcgg gcgagacggt tctgctcaag agcagcgacg gctacgcaac
     1501 gccggccttg acgctcccgc ccgaaccgac gccgacgccc acgccgctgc cgctagtgcc
     1561 ggagaaaacg gacctcttga gctccacgtc gctgccgttt ggcctggtga cgcgaatcct
     1621 gggcgacgac gagcaggaca acctgggcga gtcgatcgca ttcggggatt tcgatggcga
     1681 cgggtatgac gatctggcgg tctccagcct gttcggcggg ctgggtgaag ggttcgggtg
     1741 ggcgcggggc gacgtggtgg tgctgttcgg gcgccgggat ccgccgctgg tcgtcgatct
     1801 gaacaccggt ggcgcggcgc gggccggcga gagccggctg gggggcgaga tggatttcgg
     1861 gctgtttgga ttcgccctgg ccgccggcga catcaacggc gacggctacg acgacctcgt
     1921 catcggggcg catggcgaat cgcccggggg gcgatccggc gccgggacgg tgtatgtcgt
     1981 ctacgggcga acggacgcgg cgggtcgggt cgtgagcgtg aaccccgacg gcgcgccggc
     2041 gcagggggca acgtgcatcc tgggcgcggc ggaggaagac tggctgggtt cggcgctcgc
     2101 ctgcgccgac atcgacggcg acggcgatga cgatctgatt ctcgtcgccg ggggccacga
     2161 cggcccggag cggccggacg cgggggcggt ctaccgcatc cccggcgggg ccgacctgcc
     2221 cgggcagaga atcgacttgg cggacccgcc ggggcattat ggcgaaacgg tgatcctggg
     2281 ggtgcgggag acggatttcc tcggcaacgc ggtggcggcg gcagatctgg acggcgacgg
     2341 gcggggcgag atcatcattg gcgcgccggg gctggagtat gcgttccgcc ccaatgcggg
     2401 gggtgtctgg gtgattcccg ccgacgcggg ggaggcggtg ggcggaggcc ggcgggtgga
     2461 cctggccgcg cccgcggcgg cgtgggggat cacgctgatc gccgggcgag cgggggatca
     2521 actcgggacg agcctggcga cgggggacgt caacggcgac gggcgggccg atttgatcgt
     2581 ctcggcgccg acggccgacc gcgacggcaa ggcgctcgcc ggcgaagcct acatttttcc
     2641 cgggggcgag gcctcgccgc acggctgggc ggggggcgtg gtggacctca ggcggggcgt
     2701 cggcgcccag ggtgaaaccg ttatctattc gatgctggcc cgtgacacgc tcggttaccg
     2761 cgtggcggcg ggggacgtcg acggcgacgg gattgacgac atcctttaca gtcgcgcggg
     2821 aatggagcag ccggcgcccg acacgcgcgg cgtgatggaa atcctgctgg ggcgccgggg
     2881 cgggatcggc gccgccgccg gaagcgggcg gatcgtggcg gggtcggacc tgcgcgtcgc
     2941 cggcgaacag gtgaattcgt cgtttgccta cgccctgggc gccggcggcg acatgaaccg
     3001 caacggcctg ccggaatggg cggtcagcgc accgctgggc gacaatcccg gcgtcgcggg
     3061 gtttgccgac aacaacgccg gccgcgccta cgcgctggga gacctggcgg gaacggaaag
     3121 cgcacgggcg gcggcagtgc atcgcgcggg cgacgcgccg atccggggca tcgggggccg
     3181 gctgacgccc gcgctgcgct gtctggtcgg ctaccagggg ggcgacgccg cctcgattac
     3241 gacggcgacg ctggtgcgga cgccggcggc acgggcggag gcccacccgc cgccgggggc
     3301 ggcgcccggg ctgtggcatc tggagacgga ccgggagggg tggacctcgg cgcgggttgc
     3361 gtttgatttc accgacgggg agctggccgg gctcgatccg gcgcaattgc ggctgctgcg
     3421 ggcgccgtcg gcggctggcc cgtgggttcc ggttgcggag gccgcgttcg acgcgcggcg
     3481 gttgcgcctg agcgccacgg tggaggcgct gggctggttc tgcttcgggc cggacctgct
     3541 gacggcgcgc gtggaacagg gagcgaacca gaccgacccg gcgcacggcg ttccgatcgt
     3601 gttcgacgtc acgttcagcg cgccagtgga tgatttcgcg gccgaggacg tcacgtttgc
     3661 gggctcggcg aaggttctgg agtactcgct gtcgggggcg gctgcaagtt acgggctggc
     3721 ggtgcatcgc gcgacggccg gcgagatcat tcccgtgatc gcggccggcg tggcgcacga
     3781 cggcgacggc cgggcaaacc aggccgcggt cgggatcgat gcgtgcgtta cgtatcagcc
     3841 ggcgcccgat gtcgcgctcc gggtgggcgg ggttgcgctg gaaaacgggg aaacctacgc
     3901 cgcgccggag acgctccagg gaagtccgga cgcgcgcctt gagattgagc tggagaatag
     3961 cggggaggag gtcctgaggg tctggggcgt ggcgctggcg cccccgttca gactgctggg
     4021 ggagcccccg gagacagtcg acccggggca agcggcgcgg atgacagcgg cgattgatac
     4081 cggggtgcgc gggacattca cggcggcgtt tggtttcgag acgaacgacc cgaacgagtc
     4141 gcgggtgacg gttcagcttg aggcgcgggt ggtggggccc gatctgacgg tcgcgcggga
     4201 gccgatcgac ttcggcctgt gggatcagga tggcacgctg gtcgacgacg tgtggctggc
     4261 ggtggttaac agcggcgagc ggacgctgga gatacccgaa accaggctga tcgggccgga
     4321 tgccgacgcc tttgtggccg agccgccgcc gacgccgatc ggctcgggcc ggcagtccgc
     4381 gatccgggtg cgttacgctc cgcgcgatct gggcgcccac gaggcggtat tgtgcatcct
     4441 gagcaacgac tacgacacga ccgaggtgct gatcgcggtg cgcggggaga cgacgtcaag
     4501 gcccgacctg cgggtggggt tctcgccgcc ggacaccccc ctcgagcccg gcgtcgactt
     4561 tgaactggga ttcagttacg gcaacgcccg tccgcggacg gcgacgggtg tcgcgttgag
     4621 gctggagctg gcggacggat tgcaactgat tccgcagtcc ggcggcgagc cctgggttca
     4681 ggaaggcgag gggcgctacc tgctgaacct gaaatcgctg gggcagggga ccatccacag
     4741 ccggctggtg gtccggagcg cggagcgcat cccggcgggg gtggaaagtt accgggttcg
     4801 gctgagcatc gaggacgatg ggttgaatgg ggctgactcc cgcaccggga acaatgcggc
     4861 gacggtcgat ctccccgttg cggcgcagcc cgatctgacg ttgcgcatgg acatgctgac
     4921 gacgcgcgtg ctggccgggt atcccctcgt ggtgagcctg tgcgccggca accgtggaaa
     4981 ccaggatgcg acgaacgtgg aggtgcgtgt cgaactgccc gagccgatga cgttccggga
     5041 agacctgagc acgccgggat ggcggccggc agggggccgg gggctggagt tcgcgctggc
     5101 ggagcttgcg agcgcgacca gccggacgct cgagctgggg ctggagctgg cgctggcggc
     5161 gccgcgcgcg agcgcgttcg tgacgcttgc ggcgatggcg accgatgacg gcgcgaatgg
     5221 agccgatccc gtcccgagcg acaacgcggc cgaagcgcgg attccgtgcg catggggcat
     5281 gggggggctg gcgctcgttg ccaatacgcc gagtgccgcg tgggcggtgg ccgggccgct
     5341 cgatttcgac cggtcgggaa ccggctccgc gacgctggag tgcgtggcgg gggagtatgc
     5401 cgtgcgctgg cattccaacc cctactgggc ggcgccggag ggcgggccgg tcgccgcgac
     5461 ggtcgatgtg gatcaagtga cgaccgtcgc ggcgtgctac gagcgcctgc gcggatcgat
     5521 ccatgtgcgc gtgttgccgg gggagacgcg ctggcggctg gacggcgagg ggacgtcgtt
     5581 cgaaggcgag ggagagcggc tgctgacgga tctgccggcg gggatccatg cgtttcggcc
     5641 ggcgccgccg gcgggatatc tcaagccgta tgagtcacgg ccggtcgcgg tgccgggggg
     5701 cgcgcgcgtg aacctgctgg agatcttcat gccggcggaa gcggggccgg agcaggtcga
     5761 ggcgcggttg ctgcggtgtc tgctggggct ggattcgccc gcggaggatc tcgatttgaa
     5821 tggggatggg ttgatcgacg ccggggatct gattcagtgc gtgagggagc aagatccggc
     5881 ggcgccggcg atgagacggt gaccgggggg gtggtcccac caacgggggc agattatttg
     5941 cggatgccct ggggctgttc gagtttggac caggtgatat gc
//
LOCUS       DAOPCO010000249         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11642_length_5965_cov_4.639425, whole genome shotgun sequence.
ACCESSION   DAOPCO010000249 DAOPCO010000000
VERSION     DAOPCO010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11642_length_5965_cov_4.639425"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PLS90_13955"
     CDS             <1..184
                     /locus_tag="PLS90_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPK03548.1"
                     /translation="LRGGDVIGDHTMILAGPHERLELTHRAQTRELFAAGALRAARWL
                     GGRAAGFYSMDDLLGL"
     gene            250..2838
                     /locus_tag="PLS90_13960"
     CDS             250..2838
                     /locus_tag="PLS90_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03549.1"
                     /translation="MPVVASPSYGKEPTLSPWLVLLTPILVLLIYIGVVDNPLIGPGQ
                     ELTELNPQVTGELRWREVWLNSTPAMERGPARYRPIFAALLRLEWRAWGASPSSYHSF
                     SLILLVGLLGAGMAFFRLLAPAGLARTLAVLLLVIHPMLTESVLSVAGQGILLGLGLA
                     LGALVVVLLQRRGRIDLASATALVFVMAGLAYGCHEMALVLPLWIGVVLLLAPVPVPE
                     TTPARRRRGKVEAESSEPGRGRGQLAGMVLLLGGLIPAGFYLALRYQALDRMLPAAAI
                     HDFMFRDVAAPAWLAAPAVVLAYLGRLLWPAHPTLFYEPAAIAGRWLLIGAGWVVLAL
                     LIGALIYCWRRRPPWALALGLLLPPLIGLSHWIPLSAFMSEMPLAFALPGFALLVSLA
                     LDALGQSVRLPLRGDLRRRAVAGVWMLAVAAMAWQAWRRGGEWYSADELYQAESARHP
                     SDPAPRIERVRLAVLQGDIEQAEALVEEVTPLASPADLNRLARLRALLYEQTGRRDQL
                     RQLMRDELESGRPPEEGHFLRLATSAHGERMRDKLQQLLEAELTAFPHSFEAVHELLR
                     LELEDQEARPAGQRDWNRALDLADQMMAAAASVNDESRARAMALYGKTLANAGYLDEA
                     LQQLNQALRLDPGLYEPYLLRARIFWGRKEYQLAEDAIARAMLRARVSSYVDLAQLYV
                     GILEDQGRRDHALNWLTGALRDYPGDMALYGYAARYFIAHARYDRAQWALERMAGVQG
                     PMRADYYSALAALLWNRDRDLEGAISAARQALQINRRHPDALANLRLLEGVRDRREAT
                     PTPTPIATPAPTPVATPAIDAVTTAATTAARHADAMTPAPQVGAGPPPPQARVGEGDA
                     GTGRRE"
     gene            2913..3233
                     /locus_tag="PLS90_13965"
     CDS             2913..3233
                     /locus_tag="PLS90_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017324372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPK03550.1"
                     /translation="MSGMHPEINLPRVAIKAFCRRGSIIRLELFGSVLRDDFHADSDI
                     DFLVTFAPENRWSLLELVTMEQELSAALGRKVDLLERVSVERSHNPLRRREIQSSARP
                     IYAA"
     gene            complement(3392..4180)
                     /locus_tag="PLS90_13970"
     CDS             complement(3392..4180)
                     /locus_tag="PLS90_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HPK03551.1"
                     /translation="MKRLPTGLPWLRRLRRRKLPLLAAAFLLAVIPILIAEERRDWRT
                     ADRSSAGIAPRPEDEPRAVVQVYAARAFGWRGFFSVHCWIATKERDADHFRTFHVTGF
                     GLRRRGATVEIKSDIPDRRWYDAEPYIIQDLRGEKAAAAIPKIERAAAAYPYPATYRA
                     WPGPNSNTFTSFIIRHVPELGVELPPNAIGKDWLGPGKIFSRSESGTGFQASLFALLG
                     VTVGLGEGIEANLLGLTLGLDLWRPALKLPLIGRVGFPDAPLFD"
     gene            complement(4177..5085)
                     /locus_tag="PLS90_13975"
     CDS             complement(4177..5085)
                     /locus_tag="PLS90_13975"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK03552.1"
                     /translation="MKLAFLGMGLMGAPMAANLLKAGHQLTVYNRTRAKCEPLAQQGA
                     RVADSPADAAAGQDSVHLCIEDTADVEQVLFGERGVVQGLSAADGRPPVVVDHSTISA
                     LATEQFARRLREQTGALYLDAPVSGGDTGARAGTLAIMCGGPAPAFERVRPALEAMGQ
                     TIVHIGPNNGDGQRCKMINQLVVAINCLATTEAMRFGRAMGLDMDKVLAAISRGAAGS
                     WSLSNLGPRYLAGDFAPGFRLRHLLKDLNYCAELIAQLPDASPSDFPAVHLARELVRH
                     AESAGHGDENIHAVGRSLERSGRTNA"
     gene            complement(5107..>5965)
                     /locus_tag="PLS90_13980"
     CDS             complement(5107..>5965)
                     /locus_tag="PLS90_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03553.1"
                     /translation="PAAPPRREPERGTIAPPPPIADERRRPAPPPTAPPVHEPPPATA
                     FRPAAGQRVALPKGPPVGEFSHRHRRGPLSAMLLGILVGILLMAAIAAFGFYYWQKTG
                     EGPQEDVIARADINATIEQARLYSERGEFAKAIEQLNTLPAIPIRDSHLANIYMDKGD
                     RALTSRPQQLKAAIDAYGEAVALKPDVPRYALALGQAYYMLAISQSDDEEAMRRNLLL
                     ARQTYEALLDRHPNHLEALNELAKVAGKLKDPVLQAKTYRTIIEVAPEGSPAALEARR
                     NLESLGFKY"
BASE COUNT          852 a         1799 c         2332 g          982 t
ORIGIN      
        1 gttgcgcggg ggcgacgtga tcggcgatca tacgatgatc ctggccgggc cgcacgagcg
       61 gctggagctg acgcaccggg cgcagacccg cgagctgttc gcggcggggg cgctgcgcgc
      121 cgcgcgctgg ctgggcgggc gggcggccgg attctactcg atggatgacc tgctcgggct
      181 gtagcgctcg gccaccggaa ctctcccacg cgcatcccag aaagcggttc ttgcccagcc
      241 gaatcgtcca tgcccgttgt cgcctccccc tcctacggca aagagccgac cctttcgccg
      301 tggctcgtgc tgctgacgcc gatcctggtg ctgctgatct acatcggcgt cgtcgataac
      361 ccgctgatcg gcccggggca ggagctgacg gagctgaatc cgcaggtgac gggggaactg
      421 cgctggcggg aagtgtggct caactcgacg ccggcgatgg agcggggtcc ggcgcgctat
      481 cgaccgattt tcgccgcgct gctgcggctg gaatggcgcg cgtggggcgc cagcccgtcg
      541 agctatcatt cgttcagcct gattctgctg gtggggctgc tgggggcggg gatggcgttt
      601 ttccggctgc tggcgcccgc cgggctggcc cgcacgctgg ccgtcctgct gctggtgatc
      661 catccgatgc tcaccgagag cgtgctgagc gtcgccggtc aggggattct gctggggctg
      721 gggctggcgc tgggcgcgct cgtcgtcgtc ctgttgcagc ggcgcggccg gatcgatctg
      781 gccagcgcca ccgcgctggt gttcgtgatg gcggggcttg cgtatgggtg ccatgaaatg
      841 gcgctggtgc tgcccctgtg gatcggggtg gtgttgctgc tcgcgccggt tccggttccg
      901 gagacgacgc cggcgcggcg gcggcgtggg aaggtcgagg cggaatcgag tgaaccgggg
      961 cgcgggcgcg gccagctggc gggcatggtg ctgctgctgg gcgggctgat tcccgcgggg
     1021 ttctatctgg cgctgcgcta tcaggcgctc gaccgcatgc tacccgccgc cgcgatccat
     1081 gacttcatgt ttcgggatgt agccgcgccg gcgtggctgg cggcgccggc cgtcgtgctg
     1141 gcgtatctgg ggcgtctgct ctggccggcg cacccgacct tgttctatga gccggcggcg
     1201 atcgccggtc gctggctgct gatcggcgcg ggatgggtgg tgctggcgct gctgatcggc
     1261 gcgctgattt actgctggcg gcggcggccg ccgtgggcgc tggcgctcgg gctgctgctg
     1321 ccgccgctga tcgggctgtc gcactggatt ccgctctccg cgttcatgag cgagatgccg
     1381 ctggcgttcg cgctgccggg tttcgcgctg ctcgtttcgc tcgcgctcga cgccctcggc
     1441 cagtcggtgc ggctgccgct gcgcggcgat ctgcgccggc gggcggtggc cggggtgtgg
     1501 atgctcgcgg tcgcggcgat ggcctggcag gcgtggcggc gcggggggga atggtattcg
     1561 gccgatgagc tgtatcaggc ggaatcggcg cggcacccaa gtgacccggc gccgcggatc
     1621 gaacgggtgc gcctggcggt gttgcagggg gacatcgaac aggccgaggc gctggtcgag
     1681 gaggtgacgc cgctggcgtc gccggccgat ctgaaccggc tggcgcggct gcgcgcgctg
     1741 ctgtatgagc agaccggccg gcgcgaccaa ctgcgccagc tcatgcgcga cgagctggag
     1801 tcggggcgtc cgccggagga agggcacttt ctgcggctgg cgacgagcgc gcacggggaa
     1861 cggatgcgcg acaaactgca gcaactgctc gaggcggagc tgacggcatt tccccattca
     1921 tttgaggccg ttcacgagct gctgcggctc gaactggagg atcaggaggc gcgaccggcc
     1981 ggccagcgcg attggaaccg cgcgctggat ctggcggacc agatgatggc ggccgccgcc
     2041 agcgtgaatg acgaatcgcg cgcccgcgcg atggcgctct acggcaagac gctggccaac
     2101 gccggctacc tggatgaggc gcttcagcaa ctcaaccagg cgctgcgact cgacccgggg
     2161 ttgtatgagc cctacctgct gcgggcgcgg attttctggg ggcgcaagga atatcaactg
     2221 gccgaggacg cgatcgcccg cgcgatgctg cgtgcgcgcg tttcgtcgta tgtcgaccta
     2281 gcccagctct atgtgggtat cctggaggat cagggccgcc gggatcatgc gctgaactgg
     2341 ctgaccgggg cgctgcgcga ctatcccggc gacatggcgc tgtatgggta cgccgcgcgc
     2401 tatttcattg cgcacgcgcg ttatgaccgc gcgcagtggg ccctggaacg gatggcgggc
     2461 gtgcagggtc cgatgcgggc cgattattat tcggcgctgg cggcgctgct gtggaatcgc
     2521 gaccgcgatc tggagggggc gatttcggcg gcgcgtcagg cgctgcagat caaccggcgc
     2581 catcccgatg ccctggcgaa cctgcggctg ctggagggcg tgcgcgaccg gcgcgaggcg
     2641 acgccgacgc cgacgccgat cgccacaccg gcgccgacgc cggtggcaac cccggcgatc
     2701 gacgcggtca cgacggcggc caccacggcc gcccgccatg ccgacgcgat gacgcccgcg
     2761 ccgcaggtcg gggccgggcc gccgccgccc caggcgaggg tgggtgaggg ggatgcagga
     2821 acggggagga gggaataacg gtcgacttgg gcggaccggg cgtgtgttta gccagaagtg
     2881 gtataatgcc tggcgacgca acaggaaatg ttatgtcggg gatgcatccc gaaatcaacc
     2941 tgccccgcgt cgccattaaa gccttctgtc gccgcgggtc gatcatccga ctggagctgt
     3001 tcggctccgt gctgcgcgac gatttccacg ctgacagcga tatcgatttc ctggtgacgt
     3061 tcgcccccga gaaccgctgg tccctgctcg agctggtgac catggaacag gaactgagcg
     3121 ccgcgctcgg tcgcaaggtg gatctgctgg agcgggtgag cgtcgagcgc agtcacaatc
     3181 cgctgcggcg gcgcgagata cagtcttccg cccgacccat ttatgccgcg tgatgccgcc
     3241 tcaaccactc cgaattaccc cagtgtggtt ggaggcagaa gatattcgag agccgaggat
     3301 gagggacggg agccgagagc cgaattatgg gcgggccctt gggacattac gagaggacaa
     3361 gggacgaggg gcggaatttg gtcgggggag atcagtcgaa cagcggggcg tcggggaagc
     3421 cgacgcggcc gatcaggggg agtttgagag cggggcgcca caggtcgagg cccagcgtca
     3481 ggccgagcag gttggcctcg atcccttcgc cgaggccgac ggtgacgccc agcagagcga
     3541 agagcgaagc ctggaagccg gtgccggact cggagcggct gaagattttg cccggaccga
     3601 gccagtcttt gccgatggcg ttgggcggca gctcgacgcc cagctcgggg acgtggcgaa
     3661 tgatgaacga ggtgaacgtg ttgctgttgg gtccgggcca ggcgcggtag gtcgccgggt
     3721 agggatacgc ggcggcggcg cgctcgattt tggggatggc ggcggccgcc ttctcgccgc
     3781 gcaaatcctg aataatatag ggttccgcat cataccaacg gcggtcggga atatccgact
     3841 tgatctcgac ggtggcgccg cgccggcgga gcccgaagcc ggtgacatgg aaggtgcgga
     3901 aatgatccgc gtcccgttcc ttggtggcga tccagcagtg gacggagaag aagccgcgcc
     3961 aaccgaaggc gcgggcggca tagacctgga cgacggcgcg gggttcgtcc tcgggtcgcg
     4021 gggcgatgcc ggcgctggag cggtcggcgg tgcgccagtc gcgccgttcc tcggcgatca
     4081 ggatcgggat gacggcgagg agaaaagccg ccgcaaggag ggggagcttg cggcgccgga
     4141 gccgccgaag ccaggggagt ccggtcggca gccgcttcac gcgttcgtgc gcccggagcg
     4201 ttcaagggaa cggccgacgg catggatgtt ttcgtcgccg tggccggcgc tctcggcgtg
     4261 gcgcaccagc tcgcgggcga ggtggacggc ggggaagtcg ctcggggacg catcgggcag
     4321 ttgcgcgatg agctcggcgc agtaattgag gtccttaagc agatggcgca ggcggaagcc
     4381 cggggcgaaa tcgccggcaa ggtagcgcgg ccccaggttg ctgagcgacc atgacccggc
     4441 ggcccccctg gagatggccg ccagcacctt gtccatatcg aggcccatgg cgcggccgaa
     4501 gcgcattgcc tcggtggtgg cgaggcagtt gatggcgacg acaagctgat tgatcatctt
     4561 gcagcgctgg ccgtcgccgt tgttcgggcc gatatggacg atcgtctggc ccatggcctc
     4621 cagggcgggg cggacgcgtt cgaacgcggg cgcgggtccg ccgcacatga tggcgagcgt
     4681 gccggcgcgg gcgccggtat cgccgccgct gacgggggca tccaggtaaa gcgcgccggt
     4741 ctgttcgcgc aggcgccggg cgaactgctc ggtggcgagg gccgaaatcg tggaatgatc
     4801 gacgacgacg gggggccggc cgtcggccgc cgacaagccc tggacgacgc cccgttcacc
     4861 gaagagcacc tgctcgacat cggcggtgtc ctcgatgcag aggtggactg aatcctggcc
     4921 ggcggcggcg tcggcggggg aatcggcgac gcgcgccccc tgttgggcga gcggttcgca
     4981 cttggcgcgg gtccggttat aaacggtgag ctgatggccg gccttgagca gattggcggc
     5041 catcggcgcg cccatcaggc ccatgccgag gaatgccagc ttcatcgggg cgttcctttc
     5101 catcgatcag tatttaaagc ccagcgattc cagattgcgc cgcgcctcga gggccgcggg
     5161 gctgccttcg ggcgcgactt cgatgatcgt gcgataggtc ttggcctgca gcaccgggtc
     5221 cttcagtttg ccggccacct tggccagttc gttgagcgcc tccaggtgat tgggatggcg
     5281 gtcgagcagc gcttcatagg tttgccgggc cagcagcagg ttgcgccgca tggcctcctc
     5341 gtcgtcggac tgcgagatgg ccagcatgta gtaagcctgg ccgagggcca gggcgtagcg
     5401 cgggacgtcg ggcttgaggg cgacggcctc gccgtaggcg tcgatcgcgg ccttgagctg
     5461 ctgcgggcgc gaggtgagcg cgcggtcgcc tttgtccatg tagatgttgg ccaggtgcga
     5521 atcgcggatg gggatggcgg ggagggtgtt gagctgttcg atggccttgg cgaattcgcc
     5581 gcgttcgctg tagaggcgcg cctgctcgat ggtcgcgttg atgtcggcgc gggcgatgac
     5641 gtcctcctgg ggcccctcgc cggttttttg ccaatagtag aagccgaaag cggcgatggc
     5701 ggccatcagc aggatgccga ccagaatccc caggagcatg gcggagaggg gaccgcggcg
     5761 atggcggtgg ctgaactcgc cgaccggcgg gcccttgggg agggcgacgc gctggccggc
     5821 cgcggggcga aaggccgtgg cgggcggcgg ttcgtgaacg ggcggggcgg tgggcggcgg
     5881 agcggggcga cggcgctcgt cggcgatggg cgggggggga gcgatcgtcc cgcgttccgg
     5941 ctcacggcgc gggggcgcgg ccggg
//
LOCUS       DAOPCO010000250         5907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11744_length_5907_cov_5.272386, whole genome shotgun sequence.
ACCESSION   DAOPCO010000250 DAOPCO010000000
VERSION     DAOPCO010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11744_length_5907_cov_5.272386"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLS90_13985"
     CDS             <1..363
                     /locus_tag="PLS90_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03554.1"
                     /translation="MTDLALAMHNPRILEAFARTVAERTGAAPGYITCNFATLMASFK
                     AWGIEPAAIVAPWHPAGYGMRPDVPACEQAARACPAPIWAERRGALDAPLPEQRSHLQ
                     KIGLVGVVREDPAVWSLQ"
     gene            360..1280
                     /locus_tag="PLS90_13990"
     CDS             360..1280
                     /locus_tag="PLS90_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK03555.1"
                     /translation="MNVLVTGASGFLGAAVVLEAAAAGHTVRAMIRSAARRDRVPLPD
                     AQLVVADMRDVARFPDVVRGIQGVIHCAAVTSAGAPDEALSRLINVEGTRALLAAAAA
                     AGVARWVQISSMSAHPGSTSVYGRTKLEADEVVRRAPAPVQWTILRPSLIYGPGGRGL
                     VEKTVAILRKLPVVPVIGSGEETLRPVFVTDVARAAVICLTHERTPSRAYMLGGADEV
                     TLNSFFATLAARLGVRRPLLHLPLPLCMALARVLGTFLKNPPLTVDNVLGVRQVQPVD
                     IRPARADWGWDPIGLQEGLDRYFSATNATP"
     gene            complement(1377..1652)
                     /gene="rpsT"
                     /locus_tag="PLS90_13995"
     CDS             complement(1377..1652)
                     /gene="rpsT"
                     /locus_tag="PLS90_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012958075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPK03556.1"
                     /translation="MPNIKSAAKRMRQNIKRRAQNRSERSALRTGIKNLRAVIEQGDV
                     ETARKDLQSTLSLIGKSASKGTIHPNKARRQASRLTRKVNQMKASEG"
     gene            2072..3226
                     /locus_tag="PLS90_14000"
     CDS             2072..3226
                     /locus_tag="PLS90_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03557.1"
                     /translation="MADVHHHPQAAGSAADPAPADSRPVLWTHYGLHPEWAAARLHPA
                     LRPLRGRLEPAAPPESLDPAALADGDWDILLVGPGAASDELPNVAAVVTQLSRLRPLD
                     CLVVVEEADDTPLGHRVSLYRAGAAEILPLDATDHQLYESFRRLLGIALRRRAQIEQE
                     KLRLVGQLAVSINHEINNPLTGLMGTAELLRLESEHLGEKQRRDIQTIIEQCHRIREV
                     TARLRMLNHLRTIPYGSHDRMIDLLGEEEPARPPVAEHQPTDLFLPVPRLLVVDDNPL
                     MIDLIGRMLERRCVVHAAGAGAEALERMRQHQYDVVLVDLILPEMDGLEIVRAARRLR
                     PEQRIILMTAYQGDPRIDVARAEGVSGIIFKPFRLDEFEQILDEVLRSPD"
     gene            complement(3268..4089)
                     /locus_tag="PLS90_14005"
     CDS             complement(3268..4089)
                     /locus_tag="PLS90_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPK03558.1"
                     /translation="MQQNYYEILEIPTDASEREIKRAYHRLARDLHPDKASNPEEAKR
                     FEERFASVSAAYNVLKDAGKRAEYDRQHKTDRGAGGPSAAPRVNPTAAVLNAGRGVPG
                     SESKASGGAAAGPGRPSLGVTPEKIQIAQKAYARGMQYFKEQNYTKAIEFFDAAIQNN
                     DGEPNYHARLAVALVQAKRSATRAIEAAQRAIELDPYNLEHKFILATIFVTIGSKSNA
                     QKVYEDILRWDAGNARAQQALKELNRRTGLFSMGGGKSAAGKPGLFDQILNRFKK"
     gene            complement(4189..5106)
                     /locus_tag="PLS90_14010"
     CDS             complement(4189..5106)
                     /locus_tag="PLS90_14010"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPK03559.1"
                     /translation="MSDWRTLSLNLPETADDELAAYLTHAAVEAGALGGQLEEGRLTL
                     YLPRDADEALARAVRQSLGESARTLGFAPNALRVADRRLADAPWATAWKDRFKSFAVG
                     RRLLIRPEWERGPASTASAGEDWAGRVTIWLTPGQGFGTGHHETTRLALEWLEEELAP
                     GMTVLDFGSGSAILTIAARQLGAARVVAIECDRDANQNARENLALNGLSTAGGVELIE
                     ADTPERAAGRFDLIVCNMLPRHALAHFGALAARLAGPRSRLIYGGYLADEKGEPAQAL
                     IEAGLTPLCFRADGEWGACLAGPSAETES"
     gene            5418..5792
                     /locus_tag="PLS90_14015"
     CDS             5418..5792
                     /locus_tag="PLS90_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03560.1"
                     /translation="MPKRILAVDDETDVLLIVKTALQSEGFEVETANSGQEALPLAQE
                     NPPDLILLDVMMPGMSGFDVLRELKSHDATSTIPVIMLTGVSEKSKIAQALSSGTDFY
                     IIKPFDFDELIHKINQALDSAL"
BASE COUNT          882 a         2341 c         1684 g         1000 t
ORIGIN      
        1 atgaccgacc tggcgctcgc catgcataac ccgcgcatcc tcgaggcctt cgcccgcacc
       61 gtcgccgagc gcaccggcgc cgcgcccggc tacatcacct gcaacttcgc cacgctgatg
      121 gcctcgttca aggcctgggg catcgagccg gccgccatcg tcgccccctg gcacccggcg
      181 ggctacggga tgcgccccga cgtccccgcc tgcgagcagg cggcccgcgc ctgccccgcg
      241 ccgatctggg ccgagcgccg cggcgcgctc gacgcccccc tccccgagca acgctcccac
      301 ctccagaaaa tcggcctcgt cggcgtcgtc cgcgaggacc ccgccgtctg gagcctgcaa
      361 tgaacgtcct cgtcacgggg gcctcgggct tcctgggcgc cgcggtcgtc ctcgaagcgg
      421 cggccgccgg ccacaccgtc cgcgccatga tccgttccgc cgcccgccgc gatcgcgtcc
      481 cactccccga tgcgcaactc gtcgtcgccg acatgcgcga cgtcgcgcgc ttccccgacg
      541 tggtccgcgg catccagggc gtgattcact gcgccgccgt cacgtccgca ggcgcgcccg
      601 atgaagccct cagtcggctg atcaatgtgg aggggacgcg cgcgttgctc gccgccgccg
      661 ccgccgccgg cgtcgcgcgc tgggtgcaga tcagctccat gtccgcccat cctggctcca
      721 ccagcgtcta tggccgcacc aagctggagg cggacgaagt cgtgcgccgc gcccccgcgc
      781 cggtccaatg gaccatcctg cgccccagcc tgatctacgg ccccggcgga cgcggcctgg
      841 tcgaaaaaac cgtcgccatc ctgcgcaagc tgcccgtcgt cccggtcatc ggttccggcg
      901 aggaaacgct gcggccggtc ttcgtgaccg acgtcgcgcg cgccgccgtc atctgcctga
      961 cccacgagcg caccccctcg cgcgcctata tgctcggcgg cgccgacgag gtcacgctca
     1021 attccttctt cgccaccctc gcggcgcgcc tcggcgtccg ccgccccctg ctccacttgc
     1081 cgttgccgct ctgcatggcc ctggcgcgcg tgctggggac gttcctcaag aatcccccgc
     1141 tgacggtcga taacgtattg ggagtacggc aggtccagcc cgtcgatatc cgcccggcgc
     1201 gggcggattg gggttgggac ccgatcggat tacaggaggg gcttgaccga tacttttccg
     1261 cgacgaacgc cacgccttga cccctcacaa atgacgaaac ggacgcccgc gggcgtccgt
     1321 catcgctccg tgaaaggagg cagggccggg gccatccccc gccggaccgg atcgcttcag
     1381 ccttccgaag ccttcatctg gttcactttg cgagtcaaac gggatgcctg gcgccgcgcc
     1441 ttgttcggat gaatcgtccc cttcgatgcg ctcttgccga tcaggctcag cgtgctctgc
     1501 agatccttcc gcgccgtctc cacgtcgcct tgttcgatga ccgcccgcag attcttaatc
     1561 ccggtgcgca aggcgctgcg ctcgctgcga ttttgggcac ggcgtttgat attctgacgc
     1621 atgcgcttgg cggcggactt gatgttcggc atggcggact cgttcttctc ctcgcggttg
     1681 gcctgtattt ccctttgcaa gggattacag aaaccgccat gctatcacgt ttggttcgct
     1741 tgtcaagcgg caaggggacg tcttacgcat ccgctttgtt cctccgcgaa cgtttttgcc
     1801 gggcgcgctc cgcatcgagc ttccgaacgg cacgcgggac aggtcgaatc ccggggtccg
     1861 gccgagccgt ctggcggccg cggccggcgg ggccgcctca tccggcgacg cccctgcccc
     1921 ccgcttttcg acccgtgacg attcaaggcc agggcccggg cacatggggc gctgaccgcg
     1981 cgcgacgatc caggtccctc cgactcggac cggccgcggg cggacgaccc gggccccccg
     2041 attacgcgac atgagcgaca cggccaactc catggctgac gtgcaccacc accctcaggc
     2101 ggcggggtcc gccgccgatc ccgctccggc cgattcccgg ccggtcctct ggactcacta
     2161 cggcctgcat ccggagtggg ccgccgcgcg cctgcacccc gccctgcgcc ccctgcgcgg
     2221 acggctcgag ccggccgctc cccccgagtc cctcgatccg gccgccctcg ccgacggcga
     2281 ctgggacatc ctgctcgtcg gtcccggcgc cgccagcgac gaactcccca atgtcgccgc
     2341 cgtcgtcacc caactgagcc gcctgcgccc gctcgattgc ctcgtcgtcg tcgaggaagc
     2401 cgacgacacc cccctgggcc atcgcgtcag cctctatcgc gccggcgccg ccgagatcct
     2461 cccgctcgac gccaccgacc accagctcta cgagtccttc cgccgcctcc tcggcatcgc
     2521 cctgcgccgc cgcgcccaga tcgagcagga aaagcttcgc ctcgtcggcc agctcgccgt
     2581 cagcatcaac cacgagatca acaacccgct gaccggcctg atgggcaccg ccgaactgct
     2641 gcgcctcgaa agcgaacacc tcggcgaaaa acagcggcgc gacatccaga cgatcatcga
     2701 gcaatgccat cgcatccgcg aggtcaccgc ccgcctgcgc atgctcaatc acctgcgcac
     2761 gatcccctac ggctcgcacg accggatgat cgatctgctc ggcgaggagg aacccgcacg
     2821 gccccccgtc gccgagcacc agcccaccga tctgttcctc cccgtcccgc gcctgctggt
     2881 cgtcgacgac aacccgttga tgatcgacct gatcggccgc atgctcgagc gccgctgcgt
     2941 cgtccatgcc gccggcgccg gcgccgaagc gctcgaacgc atgcgccagc accagtacga
     3001 cgtcgtcctg gtcgatctga ttctgcctga aatggatggg ttggaaatcg tgcgcgcggc
     3061 gcgccggctg cgccccgaac agcgcatcat cctgatgacc gcctaccagg gcgatccgcg
     3121 catcgacgtc gcccgcgccg agggcgtctc cggcatcatc ttcaagccgt tccgccttga
     3181 tgaattcgag cagatcctgg acgaagtcct ccgctcgccc gattaatccc tattgtcctc
     3241 gtcctcgtcc tcggctctcg aacccgccta cttcttgaac cggttcagaa tctggtcgaa
     3301 caggcccggc ttgccggcgg cgctcttgcc gccgcccatc gaaaacagcc ccgtccgccg
     3361 gttcagctcc ttgagcgcct gctgcgcccg cgcgttcccc gcgtcccagc gcagaatgtc
     3421 ctcatacacc ttctgcgcgt tggacttgct gccgatcgtc acgaaaatcg tcgccagaat
     3481 gaacttgtgc tccaggttgt aggggtccag ctcgatcgcg cgctgcgccg cctcgatcgc
     3541 ccgcgtcgcc gaacgcttgg cctgaaccag cgccaccgcc agccgcgcat gatagttcgg
     3601 ctcgccgtcg ttgttctgga tcgccgcgtc gaaaaactcg atcgccttgg tgtaattctg
     3661 ctccttgaaa tactgcatcc cgcgcgcata cgccttctgc gcgatctgga tcttctcggg
     3721 cgtcacgccc aggctcggcc gacccggccc cgccgccgcg ccgccgctcg ccttgctctc
     3781 gctccccggc acgccgcgcc cggcgttcag caccgccgcc gtcgggttga cgcgcggcgc
     3841 cgcgctcggc cccccggccc cccggtcggt cttatgctgc cgatcatact ccgcccgctt
     3901 gcccgcatcc ttcagcacgt tatacgccgc cgacaccgag gcaaaccgct cttcgaaacg
     3961 ctttgcttcc tccggattgg acgccttgtc cgggtgcagg tcgcgcgcca gccggtgata
     4021 ggcgcgcttg atctcgcgtt cgctggcgtc ggtcgggatc tcaagaattt cgtagtagtt
     4081 ctgttgcatg ccgtctcttc aatgctgttg attcatgctg gatcggtcgg accgacgccg
     4141 cggcccgcat gccgcccggc ccgcggccgc ctgtcggctc ggccgctgct atgactcggt
     4201 ttcggctgat ggaccggcca gacaggcgcc ccattcgccg tccgcgcgaa agcacaacgg
     4261 ggtcagcccc gcctcgatca gcgcctgggc cggttctccc ttctcatcgg caagataccc
     4321 tccgtagatc agtcgcgatc gcggacccgc cagccgcgcc gccaacgccc cgaaatgcgc
     4381 cagcgcatgg cgcggcagca tgttgcacac aatcagatca aagcggcccg ccgctcgctc
     4441 gggcgtatcc gcctcgatca gttccacgcc cccggccgtg ctcaagccgt tcagcgccag
     4501 gttctcacgt gcattctgat tcgcatcgcg gtcacattca atcgccacga cgcgcgcggc
     4561 ccccagctgc cgcgccgcga tcgtcaggat cgcgctgccc gagccgaaat ccagcaccgt
     4621 catccccggc gccagttcct cctcaagcca ttccagggcc agtcgggtcg tctcatggtg
     4681 acccgtccca aacccctgcc ccggcgtcag ccagatcgtc acccgtcccg cccaatcctc
     4741 ccccgccgac gccgtcgacg ccggaccgcg ctcccattcc ggccggatca gcaatcgccg
     4801 gcccaccgca aacgacttga accggtcctt ccaggccgtc gcccacggcg cgtccgccag
     4861 ccgccgatcc gccacacgca gcgcgttcgg cgcaaacccc agcgtccggg ctgattcccc
     4921 aaggctctgg cggaccgccc gggccagtgc ctcgtccgca tcgcgcggca gatagagcgt
     4981 cagccgcccc tcctcgagct gcccgcccag cgccccggcc tcgacggccg cgtgcgtcag
     5041 gtaggccgcc agttcgtcat cggccgtctc gggcaggttc agggatagcg tgcgccaatc
     5101 ggacaacttg acaggctcta cggcggcatg ggcttgacgc acggcgggcc gatcgcctcg
     5161 cccgccgcgc aatcggactc caattctttc aacaccgccg aaccgcttca agtctcaact
     5221 tgccgcccca atccgccgcc ccatccttcc tcatcaaatc cgtccctcgt ccttgacccc
     5281 atcctcgcct ttctgtcggc caagccccgt caacgccctc cgcccgcctt gacatccatg
     5341 cgccggaaac cataatcgag gggaacgcct gttccctgga ccggcaaccg ctgcatcccg
     5401 tcgaaagctg gagcacgatg cccaagcgca ttctggccgt tgatgacgaa accgatgtcc
     5461 tgctgatcgt gaaaaccgcg ctgcagagcg aaggcttcga ggtggagacc gccaacagcg
     5521 gtcaggaagc gttgcccctc gcccaggaaa atccacccga cctgatcctc ctggacgtga
     5581 tgatgcccgg catgtcgggc tttgacgtct tgcgcgaact gaagtcccac gacgccacca
     5641 gcaccatccc ggtcatcatg ctgaccggcg tctcggaaaa atccaaaatc gcccaggccc
     5701 tctcctccgg aaccgacttc tacatcatca agccgttcga tttcgacgag ctgatccaca
     5761 agatcaacca ggcgctcgac tccgccctct gacccccctc cacccaggaa tccgtccctc
     5821 gtcctcgtaa gccgccgcct acgccttacc cgccagcttc agccggatcc cttcgccgaa
     5881 cggccgcggc tgccaccccc ccagtgc
//
LOCUS       DAOPCO010000251         5834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11925_length_5834_cov_4.849282, whole genome shotgun sequence.
ACCESSION   DAOPCO010000251 DAOPCO010000000
VERSION     DAOPCO010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11925_length_5834_cov_4.849282"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLS90_14020"
     CDS             complement(<1..403)
                     /locus_tag="PLS90_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03561.1"
                     /translation="MNSIATAFHRMHPEMAERAGFRPGPEDKPQTRRAKTPLPAVDFE
                     WPRAARARRRPAGLTLALLAVCAVIGISVGAISPGIGQVAARTAPVAATHPVELAAAE
                     GPVAAAAPAAQAATEPRAADAALASLASRLEE"
     gene            complement(403..2238)
                     /locus_tag="PLS90_14025"
     CDS             complement(403..2238)
                     /locus_tag="PLS90_14025"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPK03562.1"
                     /translation="MYLQFFGFREPPFNLTPDSKFLFLSHRHQEALAALLFGVQERKG
                     FICLTGEIGCGKTTLCRAMLSQLDQQRVRVCVVLNSYLTDLELLKTINEGYGLPSESD
                     SKKALLDRLNEFLVSEFSNGNNVVLIIDEAQNLRPDTLEQIRMISNLETETAKLIQIV
                     LVGQPELRRTLALPELEQLNQRITVRYHITPLEEREVPDYIRHRMAVAEPQVEITFSD
                     QAYRLVHHYSRGVPRRINVIVDRCLLVAYVQGTFSIDDKVVQQAVDEVRGETGGFSGG
                     PDPLLGGIGPARRTIPSWAMATALGLAAVVIIVVSIFLGQLLVQKTMSQQFAQAPGGP
                     YVNPVATMTPTPTPRAFPIEAEDALDVAPALALDEEEPTPTPTPTPTPTPTPTPRPTP
                     FLYQDWTRDTDGVLRVQDRQLAEAAAYIALLRQWNIEIDLTPFRQSSPEDVAQFDLLA
                     MVRQEPLNFEADRAEELSEALRLNMPVLVHLEERPDMAPYGVIKSMQGDILEYVDPIF
                     GERVLRRSQLGPIVREAIVLFPDEGQLAGLSSGDEGEAVTELQQRLRELGVFEGNPDG
                     KYGQRTREAVQRVQRDGGLEPTGEIDRPTAALLYARRNPQHPRLY"
     gene            complement(2506..3642)
                     /locus_tag="PLS90_14030"
     CDS             complement(2506..3642)
                     /locus_tag="PLS90_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03563.1"
                     /translation="MSSIPDASLPTPAAEPARRHPAWRGFATNLLIFLLGSLVFYLLK
                     PLALEGGDTALYTEAQWVSIFWREGFVYYNREPLAHQLVRLVYLAVGDLHQAFILCSA
                     VSGGLFLVLLRAFSRTRVVWAAVLVSCLTWNFVGHIELYGATAVVLLLYFIVLVRALE
                     PTPRATPVQAALALALAFMTHKLAFFFAPALLCLAFRRIDGRWRVRPWPARQWEKVLL
                     IIIGMCALDLLPAIANLLDIRGLLYITFTEGLLDMLTPPSRGWAFWVESRNDTGMYFL
                     FTLGSPLHLAHFFGFVVAGAPVGLVLLALNWRRIRSATAAPLVVASVIGIVWIFVWHP
                     RIGWKDWDLFSMGTLPLNLLAGGAAAGMFERRVGSAAEEGAEGT"
     gene            complement(4204..>5834)
                     /locus_tag="PLS90_14035"
     CDS             complement(4204..>5834)
                     /locus_tag="PLS90_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPK03564.1"
                     /translation="GVPADFITVADGQFVLNGQPYTAVGVTNYPFTSGNIESIERVLY
                     HARAAGITLVRAVNVFSVAHNYGAGGADFSRWNEEAHWREIDRLLDLADRLGMHVLLD
                     LSDFRTVILDGLNIDPWSADAYPYYDEMVAWVTQRVNGDNGCRYADDPTIFQWLISGE
                     PVPYGFTGNIHNMSRHVNVIEDLIFHVAEELKEHDPNHLVSAGGLLHFSGVPLDRHGQ
                     AYWRTIWSHPAIDCGAIHVYPGSFGGELTGEWTRLDEYQAYCQSIGKPLVLEEWGINQ
                     NEHDEATEIDYLTRGFDRAWELGIPVNIYWNWDPVGGYSIFTSRAEPLLAILSANAVR
                     WGAPTPFPRFAEAVITGPLLFDFEAPAEAFVSTGYGGNAGPPTISADRAWTGEHALAV
                     PVSFPSTAWNFAGVLTEFNPTRDWRGAGFIACNVWVPEHATDLSIKFMLHNVEEGWIA
                     YVQQPEGERNNWLLPGQWNQVYADLRPESLAFTRAGTAAAPALDRVVRLTLEIHHAAG
                     GAPYSGPVYIDALRLGDARTPYTSVGGHVWGRMR"
BASE COUNT         1009 a         2075 c         1769 g          981 t
ORIGIN      
        1 cctcttccag cctgctggcg agcgaagcca gcgcggcatc ggccgcccgc ggttccgtcg
       61 cggcctgcgc cgccggcgcc gcggcggcga cgggcccttc ggccgcggcc agctccaccg
      121 ggtgcgtggc ggcgaccggc gccgtgcgcg ccgcgacctg cccaatgccg ggtgagatcg
      181 cgccgaccga gatcccgatg accgcgcaga cggccagcag cgccagcgtc aggccggccg
      241 gccggcgccg cgcccgcgcc gcccgcggcc actcaaaatc gaccgccggc agcggcgtct
      301 tcgcgcgccg cgtctgcggt ttgtcctccg gaccggggcg aaaaccggcc cgctcggcca
      361 tctcgggatg catgcggtga aacgccgtgg cgatgctgtt catcagtata accgtgggtg
      421 ctgggggttg cgacgcgcat agagcagcgc cgccgtcggc cggtcgattt caccggtcgg
      481 ctccagccct ccgtcccgtt gcactcgctg cacggcttcc cgggtccgtt gcccgtattt
      541 gccgtcggga ttgccctcga acacgcccag ttcgcgcagc cgctgctgca gctcggtcac
      601 ggcttcgcct tcgtctccgg aactcaggcc cgccagttgc ccctcgtcgg ggaacagcac
      661 gatcgcctcg cggacgatcg gccccagttg cgagcgacgc agcacccgct ctccgaagat
      721 tggatcgacg tattcgagga tgtctccctg catcgatttg attacgccat agggcgccat
      781 gtcgggccgt tcctcaaggt gcaccagcac cggcatgttc aggcgaaggg cctcggacaa
      841 ttcttcggcg cgatcggcct cgaagttgag cggctcctgc cggaccatcg ccaacaagtc
      901 aaattgcgca acatcctccg gactgctctg tcggaacggc gtcaggtcga tttcgatatt
      961 ccactgccgc agcagcgcga tatacgccgc cgcctcggcc agttgccgat cctgcacccg
     1021 cagcaccccg tcggtatcgc gtgtccaatc ctgatacagg aacggcgtgg ggcgcggtgt
     1081 cggcgtcggg gtcggcgtcg gggtgggggt cggcgtcggc gtcggctcct cttcgtccag
     1141 cgccagcgcc ggcgccacat ccagcgcgtc ctcggcctca atcgggaacg cccgcggcgt
     1201 cggtgtcggc gtcatcgtgg ccaccggatt gacatacggc ccgcccggcg cctgcgcaaa
     1261 ctgctgcgac atcgtcttct gcaccagcaa ctggcccagg aatatgctca cgacgatgat
     1321 caccaccgcg gccagcccca gcgccgtggc catcgcccag ctggggatcg tccgccgggc
     1381 cggcccaatc ccacccagca gcgggtccgg cccgccgctg aagccccccg tctccccccg
     1441 cacctcgtcg accgcctgct gcaccacctt gtcgtcgatg ctgaacgtcc cctgcacata
     1501 ggccaccagg aggcagcgat cgacgatcac gttgatccgc cgcggcaccc cgcgcgagta
     1561 atgatgcacc agccgatacg cctgatcgct gaacgtgatc tccacctgcg gctcggccac
     1621 cgccatccgg tgacggatgt agtcgggcac ctcgcgctcc tcgagcggcg tgatgtgata
     1681 ccgcaccgtg atgcgctggt tgagctgctc cagctccggc agcgccagcg tccgccgcag
     1741 ttcgggctga cccaccagca cgatctgaat cagcttggcc gtctcggtct ccagattgct
     1801 gatcatccgg atctgctcaa gcgtgtcggg gcgcaggttc tgcgcctcgt cgataatcag
     1861 cacgacgttg ttcccgttcg aaaattcgga aaccagaaac tcgttgagcc gatccagcag
     1921 cgccttcttc gagtcggatt ccgacggcag cccgtaaccc tcgttgatcg tcttgagcaa
     1981 ttccaggtcg gtcagatacg agttgagcac cacgcagacg cgcacgcgct gctggtcgag
     2041 ctggctaagc atcgcgcggc acagcgtcgt cttgccgcag ccgatctcgc cggtcaggca
     2101 gatgaaaccc ttgcgctcct gcacgccgaa cagcagcgcg gccagcgctt cctgatgccg
     2161 atggctgagg aaaagaaact tggagtcggg cgtcagattg aacgggggtt cgcggaaacc
     2221 gaagaactgc aggtacatgg ggcgccatcc tggctggaag gtggagcggg cgcgaggccg
     2281 ttggccggtt gcgcccacga tcctgagggg cagccggggg cgtcgcgcga gttcactcta
     2341 tccatcgaat cggctgattt agacggtttc ccaagtccca gcggcccgat tgtgtgaaat
     2401 atcttgcgct gtcgccaatc gcgtttgcaa gaccgaacgg tctgctttcg cctttcatgc
     2461 cctttcggct ctttcgtgcc cttcgcgtct ttcgtgatcc ctccctcacg tcccttctgc
     2521 cccttcttcc gccgcggaac caacgcgccg ctcaaacatc cccgccgccg cgccgcccgc
     2581 cagcagattc agcggcagcg tccccatgct gaacagatcc cagtccttcc acccgatccg
     2641 cggatgccag acgaaaatcc agacgatccc gatcacgctg gccacgacca gcggcgccgc
     2701 cgtcgccgac cggatccgcc gccagttcag cgccagcagc accaggccga ccggcgcgcc
     2761 cgcgacgacg aacccgaaaa agtgcgccag gtgcagcggc gatccgagcg tgaacaggaa
     2821 atacatcccg gtgtcgttgc gcgactcgac ccagaacgcc cacccgcgcg acggcggcgt
     2881 gagcatgtcg agcagccctt cggtgaacgt gatgtaaagc agaccccgga tgtcgagcag
     2941 gttcgcgatc gccggcagca ggtcgagcgc gcacatgccg atgatgatca gcagcacctt
     3001 ctcccactgg cgcgcgggcc acgggcggac gcgccagcgg ccgtcgatcc gccgaaacgc
     3061 caggcagagc agcgcggggg cgaagaaaaa ggccagcttg tgcgtcatga acgccagcgc
     3121 cagcgcgagg gcggcctgca ccggcgtcgc ccgcggcgtc ggctccagcg cccgcaccag
     3181 cacgatgaaa tacagcagca gtaccaccgc cgtcgcgccg tagagctcga tatggccgac
     3241 gaaattccac gtcaggcaac tgacaagcac ggccgcccag accacccgtg tgcgactgaa
     3301 cgcccgcaac aacaccagaa acagcccgcc gctcaccgcg ctgcacagga tgaacgcctg
     3361 atgcaggtcg cccacggcca gataaaccag ccggactagc tgatgcgcca gcggctcgcg
     3421 attgtaatag acgaaccctt cgcgccagaa gatcgagacc cactgcgcct cggtgtaaag
     3481 cgccgtgtcg cccccctcca gtgcgagggg cttgagcaga taaaagacca gactgcccag
     3541 caggaaaatc agcagattgg tcgcaaaccc gcgccaggcc ggatggcggc gcgccggctc
     3601 cgccgcgggg gtgggaaggg aggcgtccgg gatcgacgac atggctgggt tgaggctccc
     3661 ctggtgacgc acggatccga tgccgcgcaa gtctgccgtt ggctggcgag cataccccgc
     3721 ccgatccggc aggtccagac cgacccacaa aaacctcgtc ccccctactc tccgtctctc
     3781 gtccctcgtc ctcgctcgaa aatttgtctc cgattcccgg gatcgcctga atccgttctg
     3841 ccgtccctac gggactcggt cgcgatgggg atcccggttc ccagcactga agtggcgggc
     3901 tgaagtctgc cgtccctgac gggactgccc tgctgttagc tccggcgagg cctggatcat
     3961 tcgaatcggc cgttcccccc caattttcat cccccgttgt gaaccgcagg ttcatgagca
     4021 acccggaatc gccccaacgg cccaccccaa gattcggctc tcggttctcg tccctcgtcc
     4081 tcggctctcg aaacccgccg atccacaccc ttcgtcgagc acttcaacta ccgtctcacc
     4141 tccctcattc ccctttcgtc ccttttgtcc cttccatccc ttcccccatc ttcttagtct
     4201 cctctacctc atcctccccc acacatggcc gcccacggac gtgtagggcg tccgtgcatc
     4261 gccaagccgt agcgcgtcga tgtaaacggg tccgctgtag ggggctcccc cggccgcgtg
     4321 atggatctcc agcgtgaggc gcacaacgcg atccagcgcg ggcgccgccg cggtccccgc
     4381 gcgcgtgaac gccagcgact cggggcgcag gtccgcgtaa acctgattcc actggcccgg
     4441 cagcagccag ttgttccgct ccccttcggg ctgttgaacg taggcgatcc agccctcctc
     4501 gacgttgtgc agcatgaatt tgatcgacag gtcggtggcg tgctcgggca cccacacgtt
     4561 gcacgcgatg aacccggcgc cgcgccagtc gcgcgtaggg ttgaactcgg tcagcacgcc
     4621 ggcgaaattc cacgccgtgc tcggaaacga aacgggaacg gccagcgcgt gctcaccggt
     4681 ccacgcccgg tcggccgaga tggtgggcgg acccgcgttg ccgccatagc cggtcgaaac
     4741 aaacgcctca gccggcgcct cgaagtcaaa caagagcggc ccggtgatga ctgcttcggc
     4801 gaaccgcggg aagggcgtcg gcgcgcccca gcgcaccgca ttggcgctca ggatcgccag
     4861 cagcggctcg gcccggctgg taaagatcga atacccgccg acgggatccc aattccaata
     4921 gatattgact ggaatcccca gttcccaggc gcggtcgaac ccgcgcgtca gataatcaat
     4981 ctccgtcgct tcatcatgct cgttctggtt gatcccccac tcctccagca ccagcggttt
     5041 gccgatcgac tggcagtagg cctggtactc gtcgagccgc gtccactcgc cggtcagttc
     5101 gccgccgaag ctcccggggt agacatgaat cgcgccgcaa tcgatggccg ggtggctcca
     5161 gatcgttcgc cagtaggcct ggccgtggcg gtccagcggc acgccgctga aatgcagcag
     5221 cccccccgcg ctcaccaggt gattcggatc atgctccttc agttcctcgg ccacatggaa
     5281 gatcagatcc tcgatcacgt taacatggcg cgacatgttg tggatgttgc cggtgaaccc
     5341 gtacggcacc ggctcgccgg agatcagcca ttgaaaaatc gtcggatcgt cggcgtagcg
     5401 gcagccgttg tcgccgttca cgcgctgtgt gacccacgcg accatttcgt cgtagtacgg
     5461 ataagcgtcg gcgctccagg gatcgatatt cagaccgtcg aggatgacgg tccggaaatc
     5521 gctcagatcg agcagaacat gcatgccgag ccggtcggcc agatcaagca ggcgatcgat
     5581 ctcgcgccag tgcgcctctt cattccagcg cgagaagtcg gcgccgcccg cgccataatt
     5641 gtgcgcaacg ctgaacacgt tgacggcgcg caccagcgtg atgccggccg cgcgcgcgtg
     5701 atacagcacg cgctcgatcg attcgatgtt gccgctggtg aagggatagt tcgtcacgcc
     5761 gacggccgtg tacggctgac cgttgagcac gaactggccg tccgcgacgg tgatgaaatc
     5821 ggccggcacg ccct
//
LOCUS       DAOPCO010000252         5820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_11949_length_5820_cov_5.613530, whole genome shotgun sequence.
ACCESSION   DAOPCO010000252 DAOPCO010000000
VERSION     DAOPCO010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11949_length_5820_cov_5.613530"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PLS90_14040"
     CDS             complement(<1..745)
                     /locus_tag="PLS90_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03565.1"
                     /translation="MQAGAARRDITPRDPVFLVGYPHVERMSTGVHDPLEASAVCLSD
                     GSRGILLLALDVLFLTRRTATRIREGICAACPIDPAAVLVSCTHTHSGPVTHKLLAWS
                     DDPVVPEPDPAYLDWLVARAVEAGVAAWSARRPARLRWASARAPLARTNRLDPTGAED
                     HRIGLLQARDAATGATLALVVIHGLHPTVLHEDSTEISADFPGFMRARLHGALGRDAV
                     IAYHTAPSGNLSPRYIVRDQTFDSARELGF"
     gene            complement(869..945)
                     /locus_tag="PLS90_14045"
     tRNA            complement(869..945)
                     /locus_tag="PLS90_14045"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(909..911),aa:Asp,seq:gtc)
     gene            complement(1158..2363)
                     /locus_tag="PLS90_14050"
     CDS             complement(1158..2363)
                     /locus_tag="PLS90_14050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03566.1"
                     /translation="MRATPSAPERIHVVHCIGTLRLGGAEKQLAELIRRLPPDRFEQS
                     LVLLQGGGPLLEDVRAAGCEVIELGWNQRFRKWNPRCWLALARVLGRYVRFLRRRRPR
                     ILHAQLYWANNLSVLAGRLARVPVIVTSRLQLSHYKRGRPLLQWIENLANRYTTAVFA
                     NSEAVKADALAHERLNPEIVRIIYNGVLLEAFERPDPAPLRRELGIEPGRVVVVAVAN
                     LHPYKGHDDLLRAAARLAPRRPELLLLLPGRDQGMRAELERLIDELKLGGQVRLLGER
                     SDIPALLALAEIVVHPSHEEGFSNAILEAMAAGRPVIVSNVGGNPEAVIDGETGLLFP
                     ARDAAALAEALERLLADRALRQRLGAAARRRVQTEFSMDRLVADFARWYEELAGRAYH
                     PGGTKTPRS"
     gene            complement(2433..3218)
                     /gene="hisF"
                     /locus_tag="PLS90_14055"
     CDS             complement(2433..3218)
                     /gene="hisF"
                     /locus_tag="PLS90_14055"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPK03567.1"
                     /translation="MLAKRIIPCLDIKDGRVVKGVNFVNLVDAGDPVQCAAAYDRAGA
                     DELVFLDITASHERRDIMIDVVRRTAEQCFMPVTVGGGLRTLENINAMLRAGADKVSL
                     NTPAVQRPPLVAEAAARFGSQCIVVAIDARRRGETAAAGWEVFIHGGRTSTGRDAIAW
                     AREAEALGAGEILLTSMDCDGTQQGYDLELTAAVARAVSIPVIASGGVGNLRHILEAF
                     TLGHADAALAASIFHFGAYSIGQCKAYLAEHGVPVRPVAEAAS"
     gene            complement(3735..5099)
                     /locus_tag="PLS90_14060"
     CDS             complement(3735..5099)
                     /locus_tag="PLS90_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03568.1"
                     /translation="MLLSVCASGAGAAGVTLFTHGSRPPGTEGRPVWLEAMRDAVADA
                     RLDGERIYGEIIVSGFPDPTARVENWLGGLGDAVHGEALVLLDWSDVANHLDNGVAVD
                     QVAAAAVDQLTEGQASYPPLVELPLHFIGHSRGTPLLTDLARRLGERGIIVDHLTSLD
                     SHPLTTNDPFQPDPPVLDPPSQIFGNILFAENLYQQEAPYPQGESVAGACNRLFSNLT
                     GGYSGFAGPHSNTHLLYHGTIDLATPVDDGEAQLGAAERMAWFNAYESPGGIGGARAG
                     FAYSRIARAADRRATAAPVAGGDRPIDGYHADPVLGGAGGRVAVDWSQAVWPNLVRLE
                     LWRDGQRLGPGPVEIIEGVPVEVRYVVFDADSGVSINFGLDIDRNPYNDNELPPFDFV
                     MHDAAGPQFVAHATTWVPAPLPLDRATYVFARISDGPRRRYLYADVDIQFVSRTGVAQ
                     WRGY"
     gene            5317..>5820
                     /locus_tag="PLS90_14065"
     CDS             5317..>5820
                     /locus_tag="PLS90_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03569.1"
                     /translation="MESTTTSAPSSRSVFELLGVQYEHLKLADGADLYLTRHGQLWRE
                     QLMPENWYAEDWFRKHRVRLRGTSTVYRVPTRPAAGRRGRPLQIVVKWSRVGQEVPLE
                     TRVIEEMLSAEFNSPFEEFSLVEELRASRRGGWPWRMLTHRPLAIYVPPEKLQLWQTG
                     RSRGKINK"
BASE COUNT          895 a         2091 c         1952 g          882 t
ORIGIN      
        1 cgaagcccag ttcccgggcc gagtcaaaag tctggtcgcg cacgatgtag cgcgggctga
       61 gattgccgct cggcgccgtg tgataggcga tcaccgcgtc gcggccgagc gcgccgtgca
      121 gccgcgcgcg catgaatccc gggaaatcgg ccgaaatctc ggtcgaatcc tcatgcagca
      181 cggtcggatg caggccgtgg atcacgacca gcgccagcgt cgcgcccgtc gcggcgtcgc
      241 gggcctgcag caggccgatc cgatgatcct cggcgccggt cggatcgagc cggttcgtcc
      301 gcgcaagggg cgcgcgggcg ctcgcccagc gcagccgcgc cggccgccgc gccgaccacg
      361 ccgccacacc cgcctccacg gcccgcgcca ccagccagtc caggtaggcc ggatcgggtt
      421 cgggcacgac cggatcatcc gaccacgcca gcagcttgtg cgtcaccggg ccggaatggg
      481 tgtgcgtgca actgaccagg accgccgccg gatcgatggg gcacgccgcg caaatccctt
      541 cgcgaatgcg cgtggccgtc cgccgcgtca gaaacagcac gtccagcgcc agcaacagga
      601 tgccgcgcga cccgtcgctc aggcagacgg cgctggcctc cagcgggtcg tgcacccccg
      661 tcgacatccg ctccacgtgg gggtatccca ccagaaaaac cgggtcccgg ggggtgatgt
      721 cacgccgcgc cgcgcctgcc tgcacgattc gcctcctcgt tctgattgcc gccggattct
      781 gcgccgcggg ctcttgcgcc aaaacagaaa caggaaggcg cacgccttcc tgcttcatcc
      841 tcgacgtaga tctgcatgtg aacttgaatg gcggggccga cgagacttga actcgcgacc
      901 tccggagtga cagtccggca ctctaaccaa ctgagctacg accccgcatc ctggcggcgg
      961 cttcccatcg gcatggatgt cgccgcgcaa ccagttccat agcttgctaa aaatggggtc
     1021 ggctcgtcaa gctgcaaaaa aatctcgctc accaagccgc ggcgcccatc gcgggccggg
     1081 gaataagagg acttcgcccg ccgtgtcgtg caggatcccg ctttacgcgg gccgctcaca
     1141 atccgctcaa aacccgctca cgaccggggc gtctttgtcc ctcccggatg atacgcccgg
     1201 cccgccagtt cctcatacca ccgcgcgaag tccgccacca ggcggtccat ggaaaactcg
     1261 gtctggaccc ggcggcgggc ggcggcgccg agccgttgcc gcagtgcgcg gtccgcgagc
     1321 agccgctcca gcgcctcggc cagcgccgcc gcatcgcgcg cgggaaacag cagcccggtc
     1381 tcgccgtcga tcaccgcctc gggattcccg ccgacgttcg agacgatcac cggccggccg
     1441 gcggccatcg cctccaggat cgcgttggaa aagccctcct catgcgacgg gtgcactacg
     1501 atttcggcca gcgcgagcag cgcggggatg tcgctgcgct cgccgaggag ccgcacctgc
     1561 ccgcccagct tcagctcgtc gatcagccgc tccagttcag cccgcatccc ctggtcgcgg
     1621 ccgggcagca gcagcagcag ttccgggcgc cgcggcgcca gccgggcggc cgcgcgcagc
     1681 agatcgtcgt gccccttgta ggggtgcagg ttcgccaccg cgacgacgac cacccgcccc
     1741 ggctcgatcc ccagctcacg ccgcagcggc gccgggtccg gccgctcgaa cgcttcaagc
     1801 agcaccccgt tataaatgat ccggacgatc tccggattca gccgctcgtg cgccagcgca
     1861 tcggccttga ccgcctctga attggcgaac accgccgtcg tgtagcggtt ggcgaggttc
     1921 tcaatccact gcagcagcgg gcggccgcgc ttatagtggc tcaactgaag ccggctcgtc
     1981 acgatcaccg gcacgcgcgc cagccgccct gccagcacgc tcaggttgtt ggcccagtaa
     2041 agttgcgcgt gcaggatccg cggccggcgc cgccgcagga agcggacgta gcgccccagc
     2101 acgcgcgcca gcgcgagcca gcagcgcgga ttccacttgc ggaaccgctg gttccagccc
     2161 agctcgatca cctcgcagcc ggcggcgcgc acgtcttcga gcagcggccc gcccccctgc
     2221 aacagaacca ggctctgttc gaaccgatcc ggcggcaacc ggcggatcag ctcggccagt
     2281 tgcttttcgg cgccgcccag ccgcagcgtg ccgatgcaat gcaccacgtg gatccgttcc
     2341 ggagctgagg gggtagcgcg cacgtgttgc ttccgtgaac tccggccggg cgcgccgcgc
     2401 cgcgaggggc cggggcgctg ccggcgttgg actcaggacg ccgcttcggc caccggccgg
     2461 accgggacgc cgtgttccgc caggtaggcc ttgcattggc cgatgctgta ggcgccgaaa
     2521 tggaaaatcg aggcggccag cgccgcatcg gcatgcccga gggtgaaggc ttccaggata
     2581 tgacgcaggt tgcccacgcc gcccgacgcg atcaccggga tcgagacggc ccgcgccacc
     2641 gcggcggtca gctccaggtc gtagccctgc tgcgtaccgt cgcaatccat gctggtcagc
     2701 aggatttccc ccgcgcccag cgcctcggcc tcgcgcgccc aggcgatcgc gtcgcggccg
     2761 gtcgacgtgc ggccgccgtg gatgaagacc tcccagccgg ccgcggcggt ctcccctcgg
     2821 cgcctggcgt cgatcgccac cacgatgcac tgcgagccga agcgcgcggc cgcctccgcc
     2881 acgagcgggg gccgctgcac cgccggcgtg ttgagcgaca ccttgtccgc gccggcgcgc
     2941 agcatcgcat tgatgttctc cagcgtgcgc agcccgccgc cgacggtcac cggcatgaag
     3001 cactgctccg ccgtccgccg cacgacgtcg atcatgatgt cgcgcctttc gtgcgaggcg
     3061 gtgatgtcca ggaacaccag ttcgtcggcc cccgcccggt cgtaggccgc ggcgcattgg
     3121 accgggtcgc cggcgtcgac caggttgacg aagttcaccc ccttgacgac gcggccgtcc
     3181 ttgatgtcca ggcaggggat gatccgtttg gcgagcaccg gccgggtccc ttctcgtgcg
     3241 ggctgcgtct tccgccggcg agggggaaga ccgtgtttgg ccccgattat acggaaaccg
     3301 ccggcccgga tgacagccgg aatcgtccct tgtcccccgt cctcgtgagc gctttcgcag
     3361 gtttgctcag ttttgtccct tcgctatgaa ccgcagattc atcagcaact cgtgatcggc
     3421 cccgacggca ctcctcagaa ttcggctccc ggctctcgtc tctcgtcttc ggctctcaag
     3481 agccatttat cattgctctc tctgttgtca ttcctcttct tctccgcgcc tcggcgtctc
     3541 ggcgtgagac ccttacgggg tgttttgcgt gtggtcggtc gcattggggg attgaatgat
     3601 cgaaccgaca aatcggttcg ggaatttcgg ccgcgccgac cgtgggttgc attggccgcc
     3661 gggcggcgcc cccgcaccgg ctcctcattg gcggagaccc agcgtatcgc cccggccggc
     3721 accttgcgcg ccgctcaata accccgccac tgcgcgactc cggtgcgcga gacgaactgg
     3781 atgtccacgt cggcgtagag atagcgccgg cgggggccgt cgctgatgcg cgcgaaaaca
     3841 tacgtcgcgc ggtcgagcgg cagcggcgcg gggacccacg tcgtggcgtg cgccacgaat
     3901 tgcggtcccg cggcgtcgtg catcacgaaa tcgaacggcg gcagctcgtt gtcgttatac
     3961 ggattgcggt cgatatcgag gccgaagttg atgctgacgc cgctgtcggc atcgaacacc
     4021 acgtagcgca cttcgaccgg gactccctcg atgatctcga ccggccccgg cccgagccgc
     4081 tgaccgtcgc gccacaattc aagccgcacc aggttgggcc agaccgcctg actccagtcg
     4141 acggcgacgc ggccgccggc gccccccagg accggatcgg cgtgatagcc gtcgatcggg
     4201 cggtcgcccc ccgcgacggg cgcggcggtg gcgcggcgat cggcggcgcg cgcgatgcgg
     4261 ctgtaggcga agcccgcccg cgccccgccg atcccgccgg gcgactcgta ggcattgaac
     4321 cacgccatgc gttcggcggc gcccagctgc gcctcgccgt cgtcaaccgg cgtcgccagg
     4381 tcgatcgtgc cgtgatacag caggtgggtg ttgctgtggg ggccggcgaa accggagtag
     4441 ccgccggtca ggttgctgaa cagccggttg caggcgccgg ccacggactc gccctgggga
     4501 tacggcgcct cctgttgata cagattctcg gcgaagagaa tattgccgaa gatctgggag
     4561 ggcggatcga gcacgggggg atccggctga aaggggtcgt tggtcgtcag cgggtgcgaa
     4621 tcgagcgagg tgagatgatc gacgatgatg ccgcgctcgc cgagccgccg cgcgaggtcg
     4681 gtcaagagcg gcgtgccgcg gctgtggccg atgaaatgca gcggcagctc gaccagcggc
     4741 ggataggatg cctgcccctc ggtcaactgg tcgacggcgg cggcggccac ctggtccacg
     4801 gcgacgccgt tgtcgaggtg attggcgacg tcgctccagt cgagcagcac gagcgcttcg
     4861 ccatggacgg cgtcgcccaa cccgcccagc cagttctcga cgcgggcggt cggatcgggg
     4921 aacccgctga cgatgatctc gccgtagatg cgttcgccgt ccagccgcgc gtcggccacg
     4981 gcgtcgcgca tcgcctccag ccagacgggc cgcccctcgg tgccgggcgg tcgggaaccg
     5041 tgggtgaaga gcgtgacgcc cgcggcgccg gcgccgctcg cgcaaaccga gagcagcatg
     5101 gccagggcga ttgcgctaca atgccggcct gaggcgcggg ctcccggcgc ggacgcgcac
     5161 ttcgccgatc cggtgtggtt catcctggca cgactccaac ggcaagtggc gtcctttata
     5221 ccccggagcg gcggcgcggg ccaagcccgc gttgcggcgg cgacgccgaa tccgccgggc
     5281 atccgaccgg cgcctcgggc gcacgggatc gaatcgatgg agtcaacgac cacctccgcc
     5341 ccctcctccc ggtcggtttt cgaactgctc ggcgtgcagt atgagcatct gaagctggcc
     5401 gacggcgcgg atctgtatct gacgcgccac ggccagctct ggcgcgagca gctcatgccc
     5461 gagaactggt atgccgagga ctggttccgc aagcaccgcg tgcggctgcg cggcaccagc
     5521 acggtctacc gcgtcccgac gcgcccggcg gcgggccgac gcggccgccc gctgcaaatt
     5581 gtcgtcaaat ggtcgcgcgt cggccaggaa gtgccgctcg agacgcgcgt catcgaggag
     5641 atgctcagcg cggaattcaa cagcccgttc gaggagttct cgctggtcga ggaactgcgc
     5701 gccagccgcc gcgggggctg gccctggcgc atgctgacgc accgcccgct cgcaatctac
     5761 gtcccccccg agaagctgca gctctggcag accggccgct cgcgcggcaa gatcaacaag
//
LOCUS       DAOPCO010000253         5763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12070_length_5763_cov_9.865452, whole genome shotgun sequence.
ACCESSION   DAOPCO010000253 DAOPCO010000000
VERSION     DAOPCO010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12070_length_5763_cov_9.865452"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLS90_14070"
     CDS             complement(<1..262)
                     /locus_tag="PLS90_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03570.1"
                     /translation="MLLVLAAAGARAENVIWTGVLDAAPTSGTSTITWTTYERADRTR
                     APWKLWMLDWASNSDGIYQVTTDQIQGRLAWLVTNPDDGATSP"
     gene            complement(400..765)
                     /locus_tag="PLS90_14075"
     CDS             complement(400..765)
                     /locus_tag="PLS90_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03571.1"
                     /translation="MTRKQWMPAAAVSALLCCAGLAAAAGYLNLGWTGIRIGDPNDLD
                     QGTVINLLEVAELEFTDDTSTTASVDVGSSYYVGGLAWIACTTDPGLTTAAVSNGTLT
                     VTTAASTTGTMNVLLLGHP"
     gene            complement(815..1285)
                     /locus_tag="PLS90_14080"
     CDS             complement(815..1285)
                     /locus_tag="PLS90_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03572.1"
                     /translation="MVSSVATDKMACRQKLQMFDHDPGATSAKLCSPDGGTTIKYLDM
                     REYTSLLVAAMLTVPGTGGSITKVEIVASADAAFTSPVVIEDPGAVAADATGDYVVAE
                     CTAEEIAQAGTDNGVDLRYAAARITMSAADAEACVSYLANPRYPQDGLTATTIA"
     gene            complement(1327..1533)
                     /locus_tag="PLS90_14085"
     CDS             complement(1327..1533)
                     /locus_tag="PLS90_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03573.1"
                     /translation="MAIEIEFIRDTREREGGPHKAGERIKAEAASAQRWLRRGAARLV
                     APTEADKSKAAAKPAEAEKKGGKN"
     gene            complement(1612..2895)
                     /locus_tag="PLS90_14090"
     CDS             complement(1612..2895)
                     /locus_tag="PLS90_14090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPK03574.1"
                     /translation="MDSKALRRKADEKLAKYQEAVGLAASEGRELSEDETRLKTDAER
                     LEAQARELEAVETQRAAYSRGVDDEPRVDAGNIQLGRDRFEDDPKRGFRNPREFLMAV
                     LNHARGGRLTEQLKSLAAGSDEQGGYSDPYGGFLVPAGFSPELLSTMAESDPISARVR
                     QIPMNAPTVAFNASVDKDHSSSVSGGLRVYRRAETDTVPASRMQFEQVRLEAHSLFGV
                     AFATEEILQDSAISFISLLESGFGKEFAAKLVDERLNGSGVGQFLGVLKSPCLITVAK
                     EGGQPADSFVYMNLVKMRAQCFGYGNAIWLANHDTLPQLLTLVNPAGQLIWQQSARDG
                     EPDRILGRPIIFTEFCDTVGDAGDIVLGDWSQYLEGTYQRPGSAESIHVRFENHERAF
                     KFYMRNDGAPWWRSALTPKNGAAQRSPFVTLAARA"
     gene            complement(2911..3777)
                     /locus_tag="PLS90_14095"
     CDS             complement(2911..3777)
                     /locus_tag="PLS90_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03575.1"
                     /translation="MRDLAQKPMRASLHAGSLAAGKVDAARRRITGYSVITRGEARGH
                     ALWIDKTFLKQVAQSGNGAAGGVRTRFNHPSFFGDGALGTALGRSTNFRVRGDQVLAD
                     LELLEAASESPKGDLAGYVLKLAQEAPDLLAASIEFSFDWVEMERFIAAHEDARGQFI
                     SPDADNTNNFIHARLVELLASDLVDEPAANPAGLFGAGDPQATEQFLAYVLGLSDEAP
                     TAALGQPERTREQVGAFLRGRGLALRAAEPAEAAANAATAEMLMAMESRLRVAMAEGF
                     QVMGQRIALRIQ"
     gene            complement(3770..5128)
                     /locus_tag="PLS90_14100"
     CDS             complement(3770..5128)
                     /locus_tag="PLS90_14100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPK03576.1"
                     /translation="MRNPLTVLRRLLAGELARDPLDERWYRDEAPAETAAGPLVTPDR
                     ALAFSAVYACVRFIAGSISILPINVYQRTGSKRRVQDEHPVQWLLHYEPHPDQTPHSF
                     RELTAAAIEMRGNAFWWKTRDALGRVKALRYLHPDLVRIKVEGGRKVFEVREDAGGWR
                     RATSEQVLHIPNFGTDGAGGLSTLACAREGVGLGVAAEQFGASFFRNDARPSVYIQSP
                     NKISDEDKARYRENWQQAQTGANRFKIAIMDQGKDLRPIQISPVDAQYIETRTFQAEE
                     ICRFFGVPPFFIGLLSKSTSWGSGLGEQKLGYLTFSLLPRLKRIEDWLDKSLLTPEER
                     RAGHYTKFNIRAFLAGDQKSQAEFLSKGREWGWYSANDCREWLEEDPIAGGDDYIRPM
                     NMQPIGAPESAGNSNDNSKGNGADEAVGEGDGFAAAMRTLNGALNGHANGSLNGRGAH
                     HA"
     gene            complement(5379..5630)
                     /locus_tag="PLS90_14105"
     CDS             complement(5379..5630)
                     /locus_tag="PLS90_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03577.1"
                     /translation="MARMVRPGKIPVRIEIDVGCKGGRMRPVEPVRPVELVAPIEIIE
                     IALEHCGRRMLVTSTLRDGEFKIRRYRCPVCGYTCKRAM"
BASE COUNT          814 a         2252 c         1628 g         1069 t
ORIGIN      
        1 tcgggcttgt cgcgccgtcg tccggattcg tcaccagcca cgccagccgc ccctgaatct
       61 gatccgtcgt cacctggtag atcccgtcgc tgttgctcgc ccagtcgagc atccacagtt
      121 tccatggcgc gcgcgtccgg tccgcccgtt catacgtcgt ccaggtgatc gtcgacgtcc
      181 ccgaggtcgg cgccgcatcc agcacgcccg tccagatcac gttctccgcc cgcgcgccgg
      241 ccgcggcgag caccagcaac attccgattg catatccgct gtttttcatt ggctttttgt
      301 tctccggcct ctccggtgat gtgggggcgc cgtccggccg gcgagcatcg cggcttgccg
      361 caccggacgg cgccttgcgg gccgggtcat ccgccggtcc tacggatgcc cgaggaggag
      421 gacattcatc gtgcccgtcg tcgacgcggc cgtcgtcacg gtcaacgtgc cgttgctcac
      481 cgccgccgtc gtcaggcccg gatcggtcgt gcaggcgatc cacgccagcc cgccgacgta
      541 atagcttgac cccacgtcca cgctcgccgt cgtgctcgtg tcgtccgtga attccagttc
      601 ggccacctcc agcaggttga tcaccgtccc ctggtccagg tcgttcgggt cgccgatccg
      661 aatccccgtc cagcccaggt tcaggtaccc cgccgctgcg gccagccccg cgcagcacag
      721 cagcgcgctc accgccgccg ccggcatcca ttgtttgcgt gtcatccgga gcatcatccc
      781 gctccctccg ttgcgattgg cattgtcatt ggaattacgc gatcgtcgtc gccgtcagcc
      841 cgtcctgcgg gtaccgcggg ttcgccagat aggacacgca cgcctccgcg tcggccgcgc
      901 tcatcgtgat ccgcgccgcc gcgtaacgca gatccacgcc gttgtccgtt cccgcctgcg
      961 cgatttcctc cgccgtgcat tccgccacga cgtaatcgcc cgtcgcgtcg gccgccaccg
     1021 cgcccgggtc ctcgatcacc accggcgacg tgaacgccgc gtccgcgctc gccacgatct
     1081 ccactttcgt gatgctcccg cccgtccccg gcacggtcag catcgccgcc accagcagcg
     1141 aggtatactc gcgcatgtcc aggtacttga tcgtcgtgcc gccgtccggg ctgcacaatt
     1201 tcgccgaggt cgcgccggga tcgtgatcga acatctgcaa tttctgccgg cacgccatct
     1261 tgtccgttgc aaccgagctc accatggtct ttctcctgct cgtgtggatt cagttcaatc
     1321 gggctgttag tttttcccgc cctttttctc ggcctccgcc ggctttgccg cggccttcga
     1381 cttgtccgct tcggtcggcg ccaccagacg cgccgcgccg cgccgcagcc accgctgcgc
     1441 cgatgccgct tccgccttga tccgctcgcc cgccttgtgc gggccgccct cgcgctcgcg
     1501 cgtgtcgcgg atgaattcga tttcgattgc cattggattt tcccccgtct ggttatacgg
     1561 ggccggggcc gcttgcgccg cgcgccccgg ccgcttgctc aggccctcgc gctacgcgcg
     1621 cgccgccagc gtcacgaacg gcgaacgctg cgccgcgccg ttcttcggcg tcaacgccga
     1681 ccgccaccac ggcgccccgt cattgcgcat gtagaacttg aacgcgcgct cgtgattctc
     1741 gaaccgcacg tgaatcgact cggccgaccc cggacgctga tacgtcccct ccagatactg
     1801 cgaccagtcg cctagcacga tgtctccggc gtcccccacc gtgtcgcaga actcggtgaa
     1861 aataatcggc cgacccagga tgcgatccgg ctccccgtcg cgcgccgact gctgccagat
     1921 cagttgcccc gccggattca ccagcgtcag cagctgcggc agcgtgtcgt ggttcgccag
     1981 ccagatcgcg ttgccatagc cgaagcattg cgctctcatc ttcaccaggt tcatgtagac
     2041 gaagctgtcc gccggctggc cgccctcctt cgccaccgtg atcaggcacg gcgacttgag
     2101 cacgccgagg aactggccca cgcccgatcc gttcagccgc tcgtccacga gcttcgcggc
     2161 gaattccttg ccgaacccgc tctccagcag cgagatgaaa ctgatcgccg agtcctgcag
     2221 gatttcctcc gtcgcgaacg cgacgccgaa caggctgtgc gcctccagcc gcacctgctc
     2281 gaattgcatc cggctcgccg gcacggtgtc ggtctccgcg cgccggtaaa cccgcaaccc
     2341 gcccgacacg ctcgacgagt ggtccttgtc cacgctcgcg ttgaacgcca ccgtcggcgc
     2401 gttcatcggg atctgccgca cgcgcgcgct gatcggatcg ctctcggcca tcgtcgacag
     2461 cagttccggg ctgaaccccg ccggcaccag aaacccgcca tacgggtcac tgtacccgcc
     2521 ctgctcgtcg ctccccgccg ccaggctctt cagttgctcc gtcagccgcc cgccccgcgc
     2581 gtggttcagc acggccatca ggaactcgcg cggattccgg aacccgcgct tcgggtcgtc
     2641 ctcgaaccgg tcgcggccca gctggatgtt ccccgcgtcg acccgcggct catcgtcaac
     2701 gccgcgcgaa tacgccgcgc gctgcgtctc caccgcctcc agctcgcgcg cctgcgcctc
     2761 cagtcgctcc gcgtccgtct tcagccgcgt ctcgtcctcg ctgagctcgc ggccctcgct
     2821 ggccgccagt ccgacggctt cctgatactt ggccagcttc tcgtcggcct tgcgccgcag
     2881 tgccttactg tccatctgtg gaccctcctg tcattggatg cgcagcgcga tgcgctggcc
     2941 catcacctgg aatccctccg ccatcgccac gcgcaggcgg ctttccattg ccattagcat
     3001 ttcggccgtc gccgcgttcg ccgcggcctc ggccggctcc gccgcgcgca gcgccagccc
     3061 gcgcccccgc agaaacgccc ccacctgctc ccgcgtccgc tccggttgcc ccagcgccgc
     3121 cgtcggcgcc tcgtcgctca ggcccagcac atacgccagg aattgttcgg ttgcctgtgg
     3181 atcgcccgcg ccgaacagcc ccgccgggtt cgccgctggt tcgtccacga ggtcgctcgc
     3241 cagcagctcc accagccgcg cgtgaatgaa gttgttcgtg ttgtctgcgt ccggactgat
     3301 gaactgtcca cgcgcgtcct cgtgcgccgc gatgaaccgc tccatctcca cccagtcgaa
     3361 ggaaaactca atcgatgcgg ccagcaggtc cggcgcttcc tgcgccagct tcagcacata
     3421 cccggcgagg tcccccttcg ggctctcgct cgccgcctcc agcagctcca gatccgccag
     3481 cacctggtcg ccccgcacgc ggaaattcgt gctccggccc agcgccgtcc ccagcgcccc
     3541 gtctccgaaa aacgacgggt gattgaatcg cgtccgcacc ccgcccgcgg cgccgttgcc
     3601 cgactgcgcc acctgtttca aaaacgtctt gtcgatccac aacgcatgcc cccgcgcctc
     3661 gccccgcgtg atcacgctgt accccgtgat ccgccggcgc gccgcgtcca ctttgcccgc
     3721 cgccaggctc cccgcgtgca ggctcgcgcg catgggcttc tgcgccagat cacgcatgat
     3781 gcgcgcccct cccgttcagg ctgccgttgg cgtggccgtt caatgcgccg ttcagcgtgc
     3841 gcatcgccgc ggcgaaaccg tccccctcgc cgacggcttc gtccgcccca ttgcccttgg
     3901 aattgtcatt ggaattaccg gcggactccg gcgcgccgat cggctgcatg ttcatcggcc
     3961 ggatgtagtc gtcgcccccc gcgatcgggt cctcttccag ccactcgcgg cagtcgttcg
     4021 cgctgtacca gccccactcg cgccccttgc tcaggaattc cgcctggctt ttctggtcgc
     4081 ccgccaggaa tgcgcgaata ttgaacttcg tgtagtgccc cgcgcgccgt tcctccggcg
     4141 tcagcaggct cttgtccagc cagtcctcga tccgcttcag tcgcggcagc aggctgaatg
     4201 tcaggtagcc taatttttgt tcgccgaggc ccgagcccca gctcgtcgat ttgctcagca
     4261 gcccaatgaa aaacggcggc acgccgaaaa accggcagat ttcctccgcc tgaaacgtcc
     4321 gcgtctcgat gtactgcgcg tcgaccggcg agatctggat cggcctcaga tccttcccct
     4381 ggtccatgat cgcgatcttg aaccggttcg cccccgtctg cgcctgctgc cagttctccc
     4441 ggtagcgagc cttgtcctcg tcgctgattt tgttcggcga ctggatgtag acgctcggcc
     4501 gcgcatcgtt ccggaaaaag ctcgccccga attgctccgc cgccacgccc agccccacgc
     4561 cctcgcgcgc gcaggccagc gtcgacagcc ccccggcgcc gtccgtcccg aaattcggga
     4621 tatgcagcac ctgctcggac gtcgcgcgac gccagccccc ggcgtcctcc cgcacctcga
     4681 aaaccttccg gccgccctcg accttgatcc gcaccagatc cggatgcagg taacgcagcg
     4741 ccttcacgcg ccccagtgcg tcgcgggttt tccaccagaa cgcattgccg cgcatctcga
     4801 tcgccgccgc cgtcagttcg cggaacgaat gcggcgtctg gtccgggtgc ggctcgtaat
     4861 gcagcagcca ctgcaccggg tgctcgtcct gcacgcggcg cttgctcccc gtgcgctggt
     4921 agacgttgat cggcagaatc gagatcgatc ccgcgatgaa ccgcacgcac gcatacacgg
     4981 ccgagaacgc cagtgcccgg tccggcgtca ccagaggccc cgccgccgtc tccgccggcg
     5041 cctcgtcgcg gtaccaccgc tcgtccagcg gatcgcgcgc gagctccccg gccagcagtc
     5101 ggcgtagaac tgtcagcgga ttgcgcaatg aaaaagcccc cagccgccca tgcatggcta
     5161 ggggctagac cggcgcgcgg atcagacgcg cctgtatcgt cgagtcattg ctacccgact
     5221 ccgctctcca acgcaaacgg gtgggctaca gatctgtcag gcataaaaaa agcccccggc
     5281 catgagccgg aggctttttt cgttgcgttg cgttgcgttg cgcggcgcgg cgaagcgctg
     5341 cgcggcgttg cggcgcgagg agcagcgggg cgatagactc acattgctcg cttgcaggta
     5401 tacccgcaca ccgggcaccg atacctcctg atcttgaatt ccccatcgcg cagcgtggac
     5461 gtgaccagca tccgccgccc gcaatgctcc agcgcgatct cgatgatctc gatcggcgcg
     5521 accagctcca ccggccgcac cggctcgacc ggacgcatcc ggcccccctt gcagccaaca
     5581 tcgatttcga tccgcaccgg gattttccct ggcctcacca tgcgcgccat ctcgctgccc
     5641 cctccccttc tcagaagatg ctcgagtact catccgccgc gtcatcatcc tggtgcttaa
     5701 tcgccaccgc cagcgcaata atcgccgcca ccacgccgtc aatcctcccc gttgaccgcg
     5761 cct
//
LOCUS       DAOPCO010000254         5681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12271_length_5681_cov_4.583185, whole genome shotgun sequence.
ACCESSION   DAOPCO010000254 DAOPCO010000000
VERSION     DAOPCO010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12271_length_5681_cov_4.583185"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..869
                     /locus_tag="PLS90_14110"
     CDS             108..869
                     /locus_tag="PLS90_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK03578.1"
                     /translation="MSPAAPHLSLVFPAYNETRRLPASLREVTAWAQAQPWPVEIVVA
                     DDGSTDGTPEQVRAEFPHCIVLDGEPNKGKGAAVARGMQAARGRFRLFSDADLSTPIG
                     EANGMLEAMARGGYDIVIASRALPESRLDVRQPPWREAAGRAFNMIVRPLSGLPFRDT
                     QCGFKLFTAQAVETIFPHQQSSGWAFDVELLMLARAFGLRVLEHPVRWINNDASKVSL
                     LSDAPRMLRDIIQFRLRILRDRPQAAQPAPRQETR"
     gene            883..2061
                     /gene="tgt"
                     /locus_tag="PLS90_14115"
     CDS             883..2061
                     /gene="tgt"
                     /locus_tag="PLS90_14115"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPK03579.1"
                     /translation="MKLFEITHRDPAGAARTGILHLRGERIETPVFMPVGTRATVKAM
                     LPDEVARIGYRLILGNTFHLELRPGSRLIERLGGLHRFMNWPHAILTDSGGFQVYSLA
                     PMRKLTEAGAEFRSPYDGQRIHLTPEEAIAIQHRLGSDIVMAFDECLDFNAPADAVRA
                     ALERTLRWLDRCRAAHAPRAGRAHLFGIVQGHFDAALRAESARRTVERDLPGYAIGGL
                     SVGEPEPLMLEMLEAAVAHLPADRPRYAMGIGLPLNLIDMAARGVDMFDCVVPTRNAR
                     NGRVYTFAGIRSIKQNQFAEDPAPLEADCPCSACRHYSRAYLRHLYQCNEILSSRLLT
                     EHNLTFFWRLMDRIRSAIRAGGLRGLRAELAAAYAAPQDAAAGGDAPDPSGPCRERSQ
                     "
     gene            complement(2400..4451)
                     /locus_tag="PLS90_14120"
     CDS             complement(2400..4451)
                     /locus_tag="PLS90_14120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPK03580.1"
                     /translation="MPDHRDPNADPPPSSPWESSEAERPDDAIEFRELGPESAPAAGP
                     PASAPADDREEPAWRPAGAGGAFEIEMFDEEREPQAGSYFAEIAPEPPPQPEELIHAP
                     ARGRKAGSGGAEPWRLGMAETLDPAVRRPQRRPPLALRLWWGLMPLILLVAGYASVIF
                     SQDGVGYAWDEAYYYRPSLQAIDWLLDLARGQTGLDQASVDYYWEERHEHPSVQKFLS
                     GFALHFFPNPESQLFAMRLPMAVLFGLTLSLIYLLGRRAWGTMPGLIAALIYLTLPRV
                     FGHAHFASMETPLVFMTLLVVFCFLRGLFSPMWAVATGVAFGLLLATKINGFFLPIPL
                     LVWAHLFARRRYVNNVFAMLFLGPVVMVAVWPWLWHDTVHRLLEYLAFHASHQQTALY
                     FLGRKWGYGGVNAPWFYPLVMIGVTLPLFSLALVIYGILRTLFRIRRRPLGALFVLCA
                     AVLLGVACAPVTPRYDGVRLFLPVFPFLALLGGAGLESLIRRIQRWTARSRRFHDLEI
                     LRVGHLLTAGAALAILAEGVTALVLYHPFYLSYFNPLVGGLEGAERRGFETTYWGEAL
                     NEEVIETLNGLPDRPDGAPPSIRPLALHGLNLAHLQEWGLLRADLRFDAEPPYDYHLL
                     LMRRGFFNRPERALADTRAFPIERRWLKFGVPMIALYRTGEAFERFWPTMPPNPPVGQ
                     E"
     gene            complement(4763..5425)
                     /locus_tag="PLS90_14125"
     CDS             complement(4763..5425)
                     /locus_tag="PLS90_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK03581.1"
                     /translation="MLRLLFPCLIVLTANAFAASSAPALSLGVETLIQAGGPEGDVIE
                     LTNRSSRGVRISSVVIDFQSSRGELIVDAGADGLGSGEWRDVVVTHAGDTGFTGITGN
                     VGDKSLDGASQIILDFSEFDAGETFRLTLDLDPLDPRKGAAVDGGLLADTMVTVYFEG
                     EDFGFVRLFGSYAEIGADAAGTTLCDERPDAPTVPEPATFGLVGLGMAGVGWFRRKKL
                     RR"
BASE COUNT          964 a         2084 c         1770 g          863 t
ORIGIN      
        1 gacatcctcg gctggaggcc gcgacacgtc accctggacg acatcgtggc ctcggcctgg
       61 gagtggcatc ggcgtcatcc ccgcggctat gaggattgag cgcatcgatg tccccggcag
      121 cgcctcacct gtcccttgtc ttccccgcct acaatgagac gcgccggctg ccggcctcgc
      181 tgcgcgaagt gaccgcctgg gctcaggcgc agccgtggcc cgtcgagatc gtcgtcgccg
      241 acgacggctc gaccgatggc acgcccgaac aggtgcgggc cgagtttccc cactgcatcg
      301 tgctggacgg cgagccgaac aagggcaagg gcgccgccgt ggcgcgcggg atgcaggccg
      361 cccgcgggcg cttccgcctg ttcagcgacg cggacctctc cacccccatc ggcgaagcca
      421 acggcatgct ggaggcgatg gcgcgcggcg gctacgacat cgtcattgcc tcgcgcgcgc
      481 tccccgagtc gcggctcgac gtgcgccagc cgccgtggcg cgaggccgcc ggccgcgcgt
      541 tcaacatgat cgtccggccg ctgtcgggcc tccccttccg cgatacgcag tgcgggttca
      601 agctcttcac cgcccaggcg gtcgaaacga tcttccccca ccagcagtcg agcggctggg
      661 cgttcgacgt cgagctgctg atgctggccc gcgccttcgg cctgcgcgtc ctggagcacc
      721 ccgtccgctg gatcaacaac gatgcctcga aggtaagcct gctgagcgac gccccccgga
      781 tgctgcgcga catcatccag ttccgcctgc gcatcctgcg cgatcggccg caggcggcgc
      841 agccggctcc gcgccaggaa acccgctgag cgcgcgccgc tgatgaagct tttcgaaatc
      901 acgcatcgcg acccggccgg cgccgcgcgc accggcatcc tgcacctgcg cggcgaacgg
      961 atcgaaaccc ccgtcttcat gccggtcggc acgcgcgcca cggtcaaggc gatgctcccc
     1021 gacgaggtcg cgcgcatcgg ctaccggctg attctcggca acaccttcca cttggagctg
     1081 cggcctggtt cgcgcctgat cgagcggctc ggcggcctgc accgcttcat gaactggccg
     1141 cacgcaatcc tcaccgattc gggcggattc caggtctact cgctcgcccc gatgcgcaag
     1201 ctcaccgagg ccggcgccga gttccgttcc ccctacgacg gccagcggat tcacctgacg
     1261 ccggaggagg cgatcgcgat tcagcaccgg ttgggcagcg acatcgtgat ggccttcgac
     1321 gaatgccttg atttcaatgc acccgccgac gccgtgcgcg ccgcgctgga acggacgctg
     1381 cgctggctgg atcgctgccg ggccgcgcac gccccgcgcg ccggccgggc gcacctgttc
     1441 ggcatcgtgc aggggcattt cgacgccgcg ctgcgcgccg agtcggcccg ccgcaccgtc
     1501 gagcgcgacc tgcccggcta tgcgatcggc ggcctgtcgg tcggcgaacc ggagccgctg
     1561 atgctcgaga tgctcgaggc cgccgttgcc cacctgcccg ccgatcgccc gcgctatgcg
     1621 atggggatcg gcctcccgct caacctgatc gacatggccg cgcgcggcgt cgacatgttc
     1681 gattgcgtcg tgccgacgcg caacgcgcgc aacggccgcg tctacacgtt cgccggcatc
     1741 cgctcgatca agcagaatca gtttgccgag gaccccgcgc cgctggaggc cgactgcccg
     1801 tgcagcgcct gccggcacta ctcgcgcgcc tacctgcggc acctgtatca gtgcaacgag
     1861 atcttgagtt cgcggctgct gaccgagcac aacctgactt ttttctggcg gctgatggac
     1921 cgaattcgta gcgcgattcg cgccggcggg ctgcgggggc tccgcgccga actcgccgcc
     1981 gcttacgccg ctccgcagga cgcggccgcc ggcggcgatg cgccagaccc ctccggcccg
     2041 tgccgcgaaa gatctcaata agagcccgcc ccatctccga cagcggctgt catcgcgcta
     2101 tttccgcgta gggcgatccc caacaggcga ttcgactgcg gatcattgga ccgtcaggct
     2161 gcccagccag tacaggtggt tccgacttgc taaccggatg agtccgcgga gcggacgacg
     2221 ggagtaaagc gcagggtgga ggccgaaggc cggaaccctg cgtcgccgcc cccaacgata
     2281 gaacccctgc aaggggtggc cggagaattc tggagccgtt cagcagccgg cgccccgcgg
     2341 tcagcgccga ccggtcgtca atgatcatcc cgttgtctca tccgtccctc caggaaatcc
     2401 tattcctgcc cgaccggcgg gttgggcggc atcgtcggcc agaaccgctc gaacgcttcc
     2461 cccgtccgat acagcgcgat catcggcacg ccgaacttca gccagcgccg ttcaatcgga
     2521 aacgcccgcg tgtcggccag cgcgcgctcg ggccgattga aaaacccgcg ccgcatcagc
     2581 agcaggtggt aatcgtaggg cggctcggcg tcgaaccgca ggtccgcccg caacagcccc
     2641 cattcctgca ggtgcgccag attgagcccg tgcagcgcca gcgggcggat gctcggcggc
     2701 gcgccgtcgg gccggtccgg cagcccgttg agcgtctcga tcacctcttc attgagcgcc
     2761 tcgccccagt aggtcgtctc gaaacctcgt cgctcggccc cctccagccc gcccaccagc
     2821 ggattaaagt aggacagata gaacggatgg tagaggacca gcgccgtaac cccttcggcc
     2881 agaatcgcca gcgccgcccc cgccgtcagc agatgcccca cccgcaggat ctcgaggtca
     2941 tggaatcgcc ggctgcgggc ggtccaccgc tgaatccgcc ggatcagact ctccaggccc
     3001 gctcccccca gcagcgccag gaacggaaac accggcagga acagtcgcac gccgtcgtag
     3061 cgcggcgtga cgggcgcgca ggcgacgccc agcagcaccg ccgcgcacag cacgaacagc
     3121 gcccccagcg gccgtcgccg aatccggaac aacgtccgca gaatgccgta aatcaccagc
     3181 gccagcgaaa agagcggtag cgtgacgccg atcatcacca gcggataaaa ccacggggca
     3241 ttgaccccgc catagcccca cttgcgcccc agaaaataca gcgccgtctg ctggtggctc
     3301 gcgtgaaacg ccaggtactc cagcaggcgg tgcacggtgt cgtgccacag ccagggccag
     3361 acggcgacca tcaccaccgg cccgaggaac agcatcgcaa agacgttgtt gacataccgc
     3421 cgccgcgcga acaggtgcgc ccacacgagc agtgggatcg gcaggaaaaa tccgttgatc
     3481 ttggtggcca gcagcagccc gaacgcgacg cccgtcgcca cggcccacat cggcgagaac
     3541 agcccgcgca gaaaacagaa caccaccagc agggtcatga agaccagcgg cgtttccatc
     3601 gacgcgaagt gcgcatgccc gaacacccgc ggcagcgtca ggtagatcag cgccgcgatc
     3661 agcccgggca tggtccccca cgcgcgccgt cccagcagat aaatcagact cagcgtcagc
     3721 ccgaacagga ccgccatcgg cagccgcatc gcgaagagct gactctccgg gttcgggaaa
     3781 aaatgcagcg caaaccccga gaggaacttc tgcacgctgg gatgctcgtg gcgctcttcc
     3841 cagtagtagt cgaccgatgc ctgatcaagg cccgtctgcc cccgcgccag gtccagcagc
     3901 cagtcgatcg cctgcaggct cgggcggtag taataggcct cgtcccatgc atagccgacg
     3961 ccgtcctggc tgaagatcac cgaggcataa ccggcgacga gcaggatcag cggcatcaac
     4021 ccccaccaca gccgcagcgc cagcggcggc cggcgttgcg gacgccggac ggccgggtcg
     4081 agcgtttcgg ccatccccag gcgccacggc tcggccccgc ccgagcccgc cttgcggccg
     4141 cgcgcgggcg cgtggatcag ttcctcgggt tgtggcgggg gctccggagc aatctcggcg
     4201 aagtagctgc cggcttgcgg ctcgcgctcc tcatcgaaca tctcgatttc gaacgctccg
     4261 cctgcgcccg ccgggcgcca ggccggttcc tcccggtcgt cggccggcgc ggacgcgggc
     4321 ggccccgcgg ccggcgccga ctccggccca agttcgcgaa actcgatggc gtcgtccggc
     4381 cgctcggctt ccgacgactc ccagggggaa cttggcggtg ggtcggcgtt gggatctcgg
     4441 tgatcgggca ttgcggcggg caacccggct gaaccgcttc cgtcgcggca tctggcattc
     4501 aggcggcccc cattctgcaa accccccggc cgattgtcga gcgcgcaacc caacgcggat
     4561 ccctgggacg cgcctcagcc cacccctccg agattctgac cgctttcgcg attccgcatc
     4621 ttgccttcga tcccggttgg ccgcgcgatg ccgcgttatc ctgtttcagt agatggattc
     4681 gaggatttga atttgtttcg aatttcgaaa ttgaagcttc gtgcttgctt ctcttgtgtt
     4741 cattcgtgtt tttcgtgatc cgctatcttc tcagtttctt ccggcgaaac cagcccaccc
     4801 ccgccatccc gaggccgacc agcccgaacg tcgccggctc ggggacggtc ggcgcgtcgg
     4861 gccgttcgtc gcacagcgtc gtcccggccg cgtcggcgcc gatctccgcg tacgaaccga
     4921 acaaccggac aaacccgaaa tcctccccct cgaaatagac ggtcaccatc gtgtcggcca
     4981 gcaatccgcc gtccaccgcc gcccccttgc gcgggtccaa cggatccaga tcgagcgtaa
     5041 gccggaacgt ctcgccggcg tcgaactcgc tgaagtccag aatgatctgc gaggcgccgt
     5101 ccaggctctt gtcgccgacg ttgcccgtga tccccgtgaa tcccgtgtcg cccgcgtgcg
     5161 tgaccacgac gtcgcgccac tcgcccgacc ccaacccatc ggcgccggcg tcaacaatca
     5221 gctccccgcg cgaggactgg aaatcaatca ccaccgaact gatccgcacg ccgcggctgg
     5281 agcgattcgt cagttcaatc acgtcgccct cgggcccgcc ggcctggatc agcgtttcga
     5341 cgccgaggct cagggcgggg gccgacgacg cggcgaacgc gttggccgtc aggacgatca
     5401 ggcacggaaa cagcagtcga agcatgaagc ggacgtatcc ttggcgaggg attggggccg
     5461 gcgacgcatg ccgccgcatg acccgaaatg cagaacccgt gccacccttc gcctgtttat
     5521 ggagaactga atcgatttga gttggacggc gtgttacaca aagtcgacac gtccgcggcg
     5581 gattcccgtt gcatccccca atcccccaaa tgtgcaaacg gttacacccc gcgcgcccgc
     5641 cccgtcacgg cagcagcact tcgtcaaacc gcgtcccggc c
//
LOCUS       DAOPCO010000255         5679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12276_length_5679_cov_5.484353, whole genome shotgun sequence.
ACCESSION   DAOPCO010000255 DAOPCO010000000
VERSION     DAOPCO010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12276_length_5679_cov_5.484353"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLS90_14130"
     CDS             <1..437
                     /locus_tag="PLS90_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03582.1"
                     /translation="GLLMLALLLLAEPIIRIGLTDLYAGAIVPLRILLPGTLMLSCSR
                     MISLYFSAQLGRPQINSIWAWVAALFYFPMIWWVVIRHEGGLIGAAAVTTAGHGILLA
                     GQWLMFMVQTGLWNPAPYLLPQPRDWGRLRRLITETIARRGL"
     gene            784..2394
                     /gene="rpoN"
                     /locus_tag="PLS90_14135"
     CDS             784..2394
                     /gene="rpoN"
                     /locus_tag="PLS90_14135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPK03583.1"
                     /translation="MALNLQQVQKQTQRLIMTPQMQQSIQLLQLNAIELEQLATQELM
                     ENPFLTIDEDVPEDQERAEDRREDARIEADGENLTAEDGGDLFQEAPADSPAEVAAAA
                     AETDEAAREREAEPPRDPETTEIADVPEAPDLEKAPEQFDEVDLNWDEYYSDAENSSY
                     AGTSEEIEERDFSEYVAARSSLYDNLTWQLRCSALEGVDCQICEYLIGNLDEDGYLRP
                     EVVTEAADEFKVEPARVERVLAILQEFDPPGIGARSLAECLLIQMKQLGSYSELARKV
                     LEEHMTTFQKKKFRELARKLEVGEEELIDLYHKVSRLEPHPGRSMSTESVHYITPDVY
                     VKVIDGELMIYLNEGQTGKLSVDRFYRRMMHQRRNALTQQEKEYAVEKYRSALTLIKN
                     IEKRKSTILRVTEAIMEVQRAFLDKGVEALRPLTLREVADMVGMHESTVARVTSSKYV
                     ETPQGVYELKFFFSSSIESEGSEAVSSRAIKEKLQHIVAAEDPRRPLSDQKIAEMLNQ
                     QGFDIARRTVAKYREQLKILPAKYRRQT"
     gene            2514..3926
                     /locus_tag="PLS90_14140"
     CDS             2514..3926
                     /locus_tag="PLS90_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03584.1"
                     /translation="MRLGFGGMRGRLAGAVGLIVATWILIGAPPAQLPAQLPLPPTDG
                     APSSPTHTYRSLVPRPDDLPTTFGRIAARRDEPTTAATSPSDAPTTFPLALDVDAPTS
                     ASMAPAPVPTPVFSIEDYPSSIGLTIDRTALWGAASDVVTSLSGVGAFTVLRDPEHDP
                     WRYRAWFDAPTTAGLAGASRSIFTARARLVEGPWLVWAGEDEWVEPARIDRHRPVILP
                     GGQYYDAIAAGDAHVVKFDSTLHLTYSSTGPNADGVPGGQPGDIDGSIRGIMGGRSSD
                     GLAWELSPTPLLLKASDFAGLPLVEGQWHEMGSYRHPALVRDGDRWRIWFDYATHDGP
                     ALGYAENLGDFVAAGDWLILNGDRSPLARGLERPTVVNVDGVWYGYARLDPRSGHPRP
                     GGWLESISLDGHTWRTLGFLELPGAADDGGLRALAVERDEGWLVHLFYPAPSAHGLTS
                     IRMLQRAIPSSERDHLPPTP"
     gene            complement(4084..>5679)
                     /locus_tag="PLS90_14145"
     CDS             complement(4084..>5679)
                     /locus_tag="PLS90_14145"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPK03585.1"
                     /translation="APVESLTPVDGFRSLASLRARYAALALEHDFITPQQVGQSRNGR
                     PIDAYLFATADEADVEGRPKPAAMLNGTIHAREWASPEVVAAVAEWLAGEQATDPVAR
                     YLIENTTTLILPVGNPDGFQVTQTYPDQTFDGGTTGASGSDGRMRRKNLRDADEMLTT
                     AADRFNGVDLNRNHPVGWSSASPLSAGYGGPSPGSEAETAAFAAAAGLLAPGRLRFYV
                     DIHSYSQLYYVIHDSHPRRNALCDRAHGLMTAATLATSGRNYINPQSVNVDAPGEAIG
                     ATDEYMGKTYRCMSYTLELRPTGGSNGFILPAAQIAETRREILAALRAGLYFASGPAA
                     LIAVEVWPIEEPAAAPLFRELRRYDPESNRREIEQTWTGPIEPGARWRMVLRFNKPLR
                     ERRDDAARLLPGADGAAAPELALPGLAGAQFGAPEWLFTVGDGPDHALRYGGDSVALE
                     LDLANLAADVEGRFTMSVQCVDLAGQALDADPRTPVDWSENGWVGYEDDAGLPGIEGG
                     ADQLTRLPIGQSETGVGRSELYR"
BASE COUNT          944 a         1974 c         1846 g          915 t
ORIGIN      
        1 acgggctgct gatgctcgcg ctgctgctgc tggccgaacc gatcatccgc atcggcctga
       61 ccgatctcta cgccggcgcg atcgtcccgc tgcggatcct gctccccgga acgctgatgc
      121 tctcgtgcag ccggatgatc tcgctctact tcagcgcgca actcggccgc ccgcagatca
      181 actcgatctg ggcctgggtc gccgcgctct tttattttcc catgatctgg tgggtcgtca
      241 ttcgccacga gggcgggctg atcggcgcgg cggccgtgac gaccgccggc catggcatcc
      301 tgctggccgg gcagtggctg atgttcatgg tgcagaccgg gctttggaat ccggcgccct
      361 acctgttgcc gcagccgcgc gactgggggc ggctgcggcg gctgatcacc gagacaatcg
      421 cccggcgcgg cttgtaatcg cgccctgtgg agggcggcgc cggagcgggg tgtcggccgc
      481 gcttcgacac cgctgttcct catctcctcg taaagcagcc gtcgcgcccc aagaaggcgg
      541 cgcagcgctg ctcaatctgg cattacgctt gcttcccgac tccgtatcgt gtaagctggt
      601 cacgatctgg aacggttctt gaacgattgg caacatttaa cttaagttca agatcagtct
      661 ggccgaacag aacttccaga agaaaaattg ccggaaccct tgatgtcccg gctccagtcg
      721 gctacggtga acacgcaccg ttccccgacc cgtaactcga ctgactcgcg taaacggtct
      781 ccgatggccc tgaatctcca acaagtccag aagcaaaccc agcggctgat catgacgccg
      841 cagatgcagc aatcgatcca gttgctgcag ctcaatgcga tcgaactgga gcagttggct
      901 acccaggagc tgatggaaaa cccgttcctg acaatcgacg aggacgtccc cgaggaccag
      961 gagcgcgccg aagaccgtcg cgaggacgcc cggatcgagg ccgatggcga aaacctcacc
     1021 gccgaggacg gcggcgatct gtttcaggaa gcgccggccg acagcccggc cgaggtggcc
     1081 gccgcggcgg ccgaaaccga tgaagccgcg cgcgaacgcg aagccgaacc cccgcgcgac
     1141 cccgaaacga ccgaaatcgc cgacgtcccc gaggccccgg acctggaaaa ggcccccgag
     1201 cagttcgatg aggtcgacct gaactgggac gagtattaca gcgacgccga gaactccagc
     1261 tacgccggca cgtcggagga gatcgaggaa cgcgacttct ccgaatacgt cgccgcccgc
     1321 tcctcgctct acgacaacct gacgtggcaa ctgcgctgca gcgccctcga aggcgtcgat
     1381 tgccagatct gcgagtatct gatcggcaat ctggatgagg acggctacct gcggcccgag
     1441 gtggtgaccg aggcggccga cgaattcaag gtcgagccgg cccgcgtcga gcgcgtcctg
     1501 gcgatcctgc aggaattcga tccgcccggc atcggcgccc gctcgctcgc cgaatgcctc
     1561 ctgatccaga tgaagcagct cggttcctac agcgaactgg cgcgcaaagt cctcgaagag
     1621 cacatgacga cgttccagaa gaagaagttt cgcgagctgg cgcgcaagct ggaggtcggc
     1681 gaggaggagc tgatcgacct ctaccacaag gtctcgcgcc tggagccgca ccccggccgc
     1741 tcgatgtcga ccgaatcggt gcactacatc acgcccgacg tctacgtcaa ggtgatcgac
     1801 ggcgagctga tgatttatct caacgagggg cagaccggca aactgtcggt cgatcgtttc
     1861 taccgacgga tgatgcacca gcggcgcaat gcgctgactc agcaggaaaa ggaatacgcc
     1921 gtcgagaaat accgctcggc gctgacgctg atcaagaaca tcgagaagcg caaatcgacg
     1981 atcctgcgcg tcaccgaggc gatcatggag gtccagcgcg cgttcctgga caagggcgtc
     2041 gaggcgctgc ggcccctcac cctgcgcgaa gtggccgaca tggtcggcat gcacgagagc
     2101 accgtcgccc gcgtcacttc cagcaagtat gtggagacgc cgcagggcgt ctacgagctg
     2161 aaattcttct tctcctcctc gatcgagtcg gagggttccg aggcggtcag ctcgcgcgca
     2221 atcaaggaaa agctccagca catcgtcgcc gccgaggacc cccgccgccc actgtcggac
     2281 cagaagatcg ccgagatgct caatcagcag ggcttcgaca tcgcgcgacg caccgtcgcc
     2341 aaataccgcg aacaactgaa aatcctcccc gccaaatacc gccggcaaac ctgaccccta
     2401 tcccgacacg cggccgatct gtcgttggcc ttggtcgggt tttttcctat catgagccga
     2461 tcctgggccg ggcgcggcgc ggcggacacc atgccagaaa tgggaatcag atcgtgagac
     2521 ttggattcgg ggggatgcgc ggtcggctgg ccggcgcggt cgggctcatt gtggcgacat
     2581 ggatccttat cggcgcgccg ccggctcaac tgccggcgca gttgccgcta cccccgaccg
     2641 acggcgcgcc cagctccccc acgcacacct accgctcgct cgttccgcgc cccgacgacc
     2701 tgcccaccac cttcggccgc atcgccgcgc ggcgcgacga accgacgacc gccgccacct
     2761 ccccgtccga cgcgccaacg acgttccccc tcgcgctcga cgtcgacgcc ccgacgagcg
     2821 cgtccatggc ccccgcgcca gtgccgacgc cggtcttctc gatcgaagac tatcccagct
     2881 ccatcggcct gacgatcgac cgcacggcgc tctggggcgc cgcgtccgat gtcgtcacca
     2941 gcctgagcgg cgtcggcgcc ttcacggtcc tgcgcgatcc cgagcatgat ccctggcgct
     3001 atcgggcgtg gttcgacgcc cccactacgg ccggcctggc cggggcctcg cgctcgatct
     3061 tcaccgcgcg cgccaggctc gtcgaaggtc cctggctggt ctgggccggc gaggacgagt
     3121 gggtcgaacc ggcccggatc gaccgccatc gccccgtgat cctccccggc gggcagtatt
     3181 acgatgcgat cgcggcgggc gacgcgcacg tcgtcaagtt cgattcgacg ctgcacctga
     3241 cttactccag caccggtccc aacgccgacg gcgtccccgg cgggcagccg ggcgatatcg
     3301 acggctcgat ccgcgggatc atgggcgggc gctccagcga cggcctggcg tgggaactct
     3361 cgccgacgcc gttgctgctc aaggcgagcg atttcgccgg cctgccgctg gtcgagggcc
     3421 agtggcacga gatggggtcg tatcgccatc cggcgctggt ccgcgatggc gaccgctggc
     3481 gcatctggtt cgactacgcg acgcacgacg gccccgcgct ggggtatgcc gaaaacctgg
     3541 gcgatttcgt cgccgccggc gactggctga tcctcaacgg cgaccggagc ccgcttgccc
     3601 ggggtctgga gcgtcccacc gtggtgaacg tcgacggcgt gtggtacggc tatgcgcgcc
     3661 tcgacccacg ctcgggccat ccgcgtcccg gcggctggct cgagtcgatc tcgctcgacg
     3721 ggcacacctg gcggacgctc ggcttcctgg agcttcccgg cgcggcggac gacggcggac
     3781 tgcgcgcgct ggccgtcgaa cgcgacgaag gctggctcgt ccatctgttc tatcccgcgc
     3841 cgtcggcgca cgggctgacg tcgatccgga tgctgcaacg cgcgatcccg tcctccgagc
     3901 gcgaccacct ccccccgacg ccctgagcgg ggagaaggat gaaacgggac gagtggttcg
     3961 tcgtccttat gtcctttatg tcccttgtgt ccttgacgtc ctttcagact caatgaagga
     4021 cgaccgatca cgaggacgag agacgaaatg cgcgtgcgag gcatggcctg agccagccgc
     4081 ggctcaccga tacagctcgc tgcggccgac gccggtttcc gattgaccaa tcggcaggcg
     4141 agtcagttga tccgcgcccc cttcgatgcc gggaaggccg gcgtcatctt cataccccac
     4201 ccagccgttc tcggaccagt ccacgggcgt gcgcggatcg gcgtcgagcg cctgccccgc
     4261 cagatccaca cattggacgc tcatggtgaa gcggccttcc acgtcggccg cgaggttcgc
     4321 aagatccagc tcgagcgcga cggaatcgcc gccgtagcgc agggcatggt ccgggccgtc
     4381 tccgacggtg aacaaccact ccggcgcgcc gaattgagcc cccgcgagcc ccggcagcgc
     4441 gagctccggc gccgcggccc cgtccgcccc cggcagcaac cgcgcggcgt cgtcgcgcct
     4501 ctcgcgcaac ggcttgttga accgcagcac catccgccag cgcgcccccg gctcaatggg
     4561 gcccgtccac gtctgctcga tttcgcgccg gttcgattcc ggatcgtaac ggcggagctc
     4621 ccggaacagc ggcgccgcgg ccggctcctc gatgggccag acctccacgg cgatcaacgc
     4681 cgccgggccg gaggcgaagt acagacccgc gcgcagcgcg gccagaatct cgcgccgcgt
     4741 ctcggcgatc tgcgccgccg gcaggatgaa gccgttactt ccgccggtcg ggcgcagctc
     4801 cagcgtgtag ctcatgcagc ggtaggtttt ccccatgtat tcatcggtcg cgccgatcgc
     4861 ctcgcccggc gcgtccacat tcacggactg cgggttgatg tagttgcggc cgctcgtggc
     4921 gagcgtagcg gcggtcatca gcccgtgggc gcggtcgcag agcgcgttcc gccgcgggtg
     4981 gctgtcgtga atcacatagt aaagctgcga gtagctgtgg atatcgacgt agaagcgcag
     5041 gcgtccgggg gcgagcaggc cggcggctgc ggcgaaggcc gccgtctcgg cttcgctgcc
     5101 ggggctgggg ccgccgtagc ccgcggagag cgggctggcg ctggaccagc cgaccggatg
     5161 attgcggttc aggtcaaccc cgttgaaccg gtcggcggcg gttgtcagca tttcgtcggc
     5221 gtcgcgcagg tttttgcgcc gcatccgccc gtcgctgcca ctggcgccgg tcgtgccgcc
     5281 gtcgaaggtc tggtccggat aggtctgggt tacctggaag ccgtcgggat tgccaacggg
     5341 caggatcagc gtcgtcgtgt tctcgatcag gtagcgtgcg actgggtcgg ttgcctgctc
     5401 cccggccagc cactcggcga cggcggcgac gacttccgga ctggcccact cgcgcgcgtg
     5461 aatcgtgccg ttgagcatcg cggccggctt cggccggccc tcaacatcgg cctcgtccgc
     5521 agtcgcgaac aggtaggcgt cgatcggccg gccgttgcgg ctctggccga cctgctgcgg
     5581 cgtgatgaag tcgtgttcga gcgccagggc ggcgtaacgc gcgcgcagcg acgccagcga
     5641 gcggaacccg tcgacgggcg tcagcgactc caccggcgc
//
LOCUS       DAOPCO010000256         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12298_length_5672_cov_3.822325, whole genome shotgun sequence.
ACCESSION   DAOPCO010000256 DAOPCO010000000
VERSION     DAOPCO010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12298_length_5672_cov_3.822325"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLS90_14150"
     CDS             complement(<1..687)
                     /locus_tag="PLS90_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03586.1"
                     /translation="MNINPLLARVSLVLCLLSPALVAADHPTYGWKSSVSSIRQSHRT
                     VSLERLAIDISHLADYIYERAKDESDYARRYYDKDDVKRAIERLDSFHDTAREFRKEA
                     DDSHGRVSRDLRELHEDLVRRLHLADIRMHVFSRSLRDDFEELQLLVRALDRYFIDSG
                     PRRGHVVERRVVTPPPRHIVRETVVYCPATCDLRHTHRYSVDTLRRFPELRFRNQVDN
                     RRDWDRRDGRE"
     gene            complement(803..2230)
                     /locus_tag="PLS90_14155"
     CDS             complement(803..2230)
                     /locus_tag="PLS90_14155"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK03587.1"
                     /translation="MVPQTATPNRGRVVIFDDEPSMGRILVKTLGLEGFEARSFTNPV
                     EGFEALNELQPDVLLTDVRMPEMDGLTVLERMRRQYPDVPVLIITAYGTVEGAVAAMQ
                     AGAFNYITKPFEQANLVAQISKALEHRRVVQENERLSEQLAGQGQAREILGNSPQIAR
                     VRDMIARAAPTDSSVLITGPSGVGKELVAQAIHAQSRRAGGRFVAINCPSVPPSLIES
                     EMFGYERGAFTGADRSKMGLVELAQGGTLFLDEVAELPAEMQVKLLRVIQEREIQRVG
                     GLRQIPVDLRLIAATNRDLDEEMAAGRFREDLYYRLNVIHIEIPALSERPEDVPLLAE
                     RFMERIGRRMLRPDLRLSREALEALRQYHWPGNVRELENVIERAIVLTTGQEIRPEDL
                     TIDLRRRELAAEEGAVAGGPAGIAWPIDYRAARDMFERQYLRNLVEYAGGNISHAAHV
                     SGISRRNLYDKLERVGLDEELVRKR"
     gene            complement(2883..2969)
                     /locus_tag="PLS90_14160"
     tRNA            complement(2883..2969)
                     /locus_tag="PLS90_14160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2933..2935),aa:Leu,seq:taa)
     gene            complement(3151..3999)
                     /gene="gluQRS"
                     /locus_tag="PLS90_14165"
     CDS             complement(3151..3999)
                     /gene="gluQRS"
                     /locus_tag="PLS90_14165"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HPK03588.1"
                     /translation="MGRYAPSPTGDLHLGNLRTALRAWEDCRRRGGTFILRIEDLDPP
                     RTVPGAEARMIEDLRWLGLDWDEGPDAGGPAGPYRQSERGAIYQAALDELTRRGLTYP
                     CVCSRRDLREASAPHGEAGEPVYPGVCRSADPERLARHPGGAAVRFRLPDDAVIEFED
                     AAAGPQRFDLNRLCGDFVIRRRDGLWAYQLACAIDDALMGVTHVVRGADLLTSTPRQI
                     AVLRALGLPVPSYCHIPLVADEAGRRMCKRDGSCSIRALRAAGQSADEVRRIIMQARL
                     VEVENG"
     gene            4311..4733
                     /locus_tag="PLS90_14170"
     CDS             4311..4733
                     /locus_tag="PLS90_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03589.1"
                     /translation="MNLKCLFWCLHQLQTRYDLNLVLQSLERRAIHPEIADSNLGDNL
                     TYLLDHLAPEDLPRLAEAIDGLPLSAGSTDIMDKHFEIGHYIQSLTDFGPIRSGTYGI
                     ISAVTPQLRGLFLVEGDSLIEAPFPPESTQTIFGTYVI"
     gene            4970..5656
                     /locus_tag="PLS90_14175"
     CDS             4970..5656
                     /locus_tag="PLS90_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03590.1"
                     /translation="MLLNVLLVVHIVALLLVVTGMTPLTHNLDDIKILLFFIGGPILM
                     LLATAGIAARILPTPPRVIGASLLAYLLIAFLSTLTAQFRWESWHQMALLWSAGGMFL
                     SAMAIGSHPRLSETFLRYLVAQLLLACLIGFFFFDVTNSPNHLSGVSILHRLLYDSLR
                     PPYDNASPFRSLLWTLVNADKNMQSTILSRDFFAAFCVLYLPFALLLSLDPGPRNRLL
                     CAASAAWPPC"
BASE COUNT          908 a         2042 c         1680 g         1042 t
ORIGIN      
        1 ctcgcgccca tcacgccggt cccaatccct ccggttatcg acctgattgc ggaaccgcaa
       61 ttccgggaag cggcgcaacg tatcgaccga gtagcggtgc gtgtgacgga ggtcgcacgt
      121 cgccgggcag taaacgacgg tctcgcgcac gatgtgccgc gggggcggcg tcaccacgcg
      181 acgctcgacc acatggccgc ggcgcgggcc gctgtcaatg aagtaacggt ccagtgcccg
      241 caccagcagc tgcagctcct cgaaatcatc gcgcaagctg cggctgaaga cgtgcatccg
      301 gatatctgcc agatggagcc gacgcacgag gtcctcgtgc aactccctca gatcgcgaga
      361 aacccgcccg tgcgaatcat cggcttcctt gcggaactcg cgcgcggtgt cgtggaaact
      421 gtccagccgt tcaatcgcgc gcttcacatc atccttgtcg taatagcgcc ttgcgtaatc
      481 ggattcatcc ttcgcccgtt cgtaaatgta atccgcgagg tggctgatat cgatcgcgag
      541 ccgctccagt gagaccgtcc ggtgcgactg gcggatgctt gagaccgagc ttttccaacc
      601 gtacgtcgga tggtccgccg cgaccagcgc cgggctcagc aggcagagga ccaggctcac
      661 gcgcgccagc aaggggttga tgttcatggg ttgtacctcc cggtccggtc tcggccgttc
      721 cagcacggcc tgtctgtttg gacagccccc ccgccggttt gttcctcccc tccccccaaa
      781 ttccgtctcc aaaggctagc ccctaccgct tccgcaccag ttcctcgtcc aaccccacgc
      841 gttccagttt gtcataaagg tttcgccggc tgatccccga gacatgcgcg gcgtgcgaga
      901 tgttgccccc ggcatattca acgagattgc gcaggtactg ccgctcgaac atgtcgcgcg
      961 cggcccggta gtcgatcggc caggcaatgc cggcggggcc tcccgcaacg gccccttcct
     1021 ccgccgcgag ttcgcgccgc cgcagatcga tcgtcaaatc ctccggccgg atctcctggc
     1081 ccgtggtcag cacgatcgcc cgttcgatca cgttctccaa ttcgcgcacg ttgcccggcc
     1141 agtgatactg acgcagcgcc tcgagcgcct cgcggctgag ccgcagatcc ggccgcaaca
     1201 ttcgccggcc gatccgctcc atgaaccgct cggccagcag cggcacatcc tccggccgtt
     1261 cgctcaaggc gggaatctcg atgtggatca cgttaaggcg atagtacagg tcctcgcgga
     1321 accgccccgc cgccatctct tcatccaagt cgcggttggt cgcggcgatc agccgcagat
     1381 cgaccggaat ctggcgcaac ccgccgacgc gctggatctc gcgctcctgg atcacgcgca
     1441 gcagcttgac ctgcatctcg gcgggcaact ccgccacctc gtccagaaac agcgttcccc
     1501 cctgcgccag ttcgaccagg cccatcttcg agcgatcggc tccggtgaac gccccgcgtt
     1561 catagccgaa catctcgctc tcgatcagcg agggggggac cgacgggcag ttgatcgcca
     1621 cgaaacgccc gccggcccgg cggctctggg cgtgaatcgc ctgcgccacc agttccttgc
     1681 cgacgccgct ggggccggtg atcagcaccg acgaatcagt cggggcggcc cgcgcgatca
     1741 tatcgcgcac gcgcgcgatc tgcggcgaat tgcccagaat ctcgcgcgcc tggccctgcc
     1801 cggcaagctg ttcgctcagc cgctcgtttt cctgcaccac gcgccggtgc tccagcgcct
     1861 tgctgatctg ggccaccagg ttcgcctgct cgaacggctt cgtgatgtag ttgaacgccc
     1921 ccgcctgcat cgccgccacg gccccctcca ccgtcccgta ggccgtgatg atcagcaccg
     1981 gcacgtccgg atactggcgc cgcatccgct ccagcaccgt cagcccatcc atctccggca
     2041 tgcgcacgtc ggtcagcagc acgtccggct ggagctcgtt gagcgcctca aagccttcca
     2101 ccggattggt gaacgagcgc gcctcgaacc cctccaggcc gagcgtcttg accaggattc
     2161 ggcccatcga cggttcatcg tcgaagataa ccacgcggcc gcgattgggt gtggcggttt
     2221 gggggaccat caactgcccg ctttcgttcc ggaggcgatc caacacgccc cgcaaacttt
     2281 cgggtgactg accgtgcggg gctgggcgag ttcggtaaaa ttctaccgcc gccgccgcaa
     2341 ggctggcaag ccctcccgca tcggcgagtt gactcatcat tcaggacacc gaaggaggtg
     2401 gctgatcgat acgtcaaaac ctgcaacgga agccggcccc ggaggtattg ggcgcctcgt
     2461 gggacttcga gaatattgaa gcctacacgc ccctttgcac ctgcgagagc tgcgggatgt
     2521 ccggttccgc cgccgagatc gaagccatcg ccacgtatct ggcggcacag gagtcacgca
     2581 tcccggccca cgtgccattg acatggccat gcaaggttac gatcgcaacg gcgccttccg
     2641 ggaccacgaa gtgctgagcg acgtgaatcg tgccacttaa tttgagatgg taaaaggcaa
     2701 cccccgctac ctcgcgatgg tcgtttttaa ctggcggcgc gaaggcagtt cgtgcccggc
     2761 ccatcctgcg ccccaatggg ggcacgggcg ggagttcccg accccagcga ccttggccga
     2821 caccgaagcc cgcgaagcag acccgtgagc ggcccctcag ctcaaccgcg gagaggtgag
     2881 gctggtgccg agaccgggat tcgaacccgg acggcctttc ggccggggga ttttaagtcc
     2941 cctgcgtctg ccagttccgc catcccggca cgtcgacccg caggatatca tcccgcgcca
     3001 acccgccgcc agtttcgttg ctccagcccc ctccccgccc ctcacttcct tgccatcact
     3061 gtcggtatcg ccatcgatat cgccatcgat atcggtatcg gtatcgatag ctgccgtttg
     3121 cgctgcttct tgtgtttcct tccgttcgac tcacccattc tcaacctcaa ccagcctggc
     3181 ctgcatgatg atccgccgga cttcgtcggc cgactgcccc gccgcgcgca gcgcgcgaat
     3241 cgagcacgaa ccgtcgcgct tgcacatccg ccggccggcc tcgtcggcga ccagcgggat
     3301 gtgacaatag ctggggacgg gcagccccag cgcccgcagg accgcgatct ggcgcggcgt
     3361 cgacgtcagc agatccgccc cccgcaccac atgcgtcacg cccattagcg cgtcgtcgat
     3421 ggcgcaggcc aactgatagg cccacagtcc gtcgcgccga cggatgacaa aatcgccgca
     3481 cagccggttc agatcgaacc gctgcgggcc ggcggccgcg tcctcgaact caatcacggc
     3541 gtcgtcgggc agacggaagc ggaccgccgc ccctccgggg tgccgtgcca gcctctccgg
     3601 atcggcgctg cggcaaacgc cggggtaaac cggctcgccc gcttcgccgt ggggcgcgct
     3661 ggcctcgcgc aggtcccggc ggctgcagac gcacgggtaa gtcagcccgc ggcgcgtcaa
     3721 ctcatccagc gcggcctgat agatcgcgcc gcgctccgat tgccggtagg gacccgccgg
     3781 tcccccggcg tcgggcccct cgtcccaatc gagccccagc cagcgcaggt cctcaatcat
     3841 ccgcgcttcg gcgcccggca ccgttcgcgg gggatccaga tcctcgatcc gcaggatgaa
     3901 cgttccgccg cgccggcggc agtcctccca ggcccggagc gcggtccgca ggttgcccag
     3961 gtgcaggtcg ccggtggggc tcggggcgta gcggcccacc ggaccggttc gtttcactgg
     4021 acgaaggtca gcgctcatga cacaattccg ctcggccgtc cgccatggcg tcgcggcggc
     4081 cggtcggctc cgcggatccg gagcggcggt tggggcaagt cttgcgtttc caatcgtctg
     4141 agcgaggtcc gtgcggaggg tgggatatct ctgtcctctt ttcctgttga cgcgcaaaaa
     4201 ccttgaagca tatgatttct ttgacgacaa gtcaagccga cttgaagagt gtcgcgatgt
     4261 ctgttcccac tgcaccggtc cgccagtccc ggcagacccg aaagatcctc ttgaatctga
     4321 agtgcctctt ctggtgcctg caccagttgc agacccgcta tgacctgaac ctggtgctgc
     4381 aatcgctcga gcgccgggcg attcaccccg aaattgccga ctccaacctg ggcgacaacc
     4441 tgacgtatct gctcgatcac ctggcgccgg aagacctgcc gcgcctggcc gaggcgatcg
     4501 atgggctgcc gctctcggcc ggttccaccg acatcatgga caagcatttc gagatcggcc
     4561 actacatcca gagcctgacc gatttcggcc cgatccgctc gggcacctac ggcatcattt
     4621 cggcggtcac gccccagttg cgcggcctgt tcctggtcga gggcgacagc ctgatcgagg
     4681 cccccttccc ccccgagtcg acacagacga tcttcggaac ctacgtgatt tagccccatc
     4741 ccggcaacaa cgtctcgtcc gtcctgatga tccgcccgtt ctcgccattc tcgcgcggaa
     4801 gtggaagccc gccccggatt tgtgtaaggt ggggccggtg atgcatcccg cgtgatggtg
     4861 tgatcggtcc atggccaaac gtcgttcgtc ccgtcgcccg caggggtcga cgccgcgtca
     4921 acccgttcct ctgcctcccc tccccccacc cggcgggggc tcgccgccgt tgctgctgaa
     4981 cgtgctgctc gtcgtccaca tcgtggcgct gcttctggtg gtcacgggca tgacgccgct
     5041 gacgcacaac ctggatgaca tcaagattct gctgtttttc atcggcgggc cgatcctcat
     5101 gctgctggcg accgccggca tcgcggcgcg gatcctgccg acgccgccgc gggtcatcgg
     5161 cgcgagcctg ctggcctacc tcctgattgc cttcctcagc acgctgacgg cgcaattccg
     5221 ctgggaaagc tggcatcaga tggcgttgct ctggtccgcc ggcgggatgt tcctctccgc
     5281 catggcgatc ggctcgcacc cgcgcctgag cgagaccttt ctgcgctatc tggtcgccca
     5341 gctcctgctc gcctgcctga tcggcttctt cttcttcgac gtcaccaact cgcccaatca
     5401 cctctccggc gtctcgattc tccaccggct gctctacgac agcctgcgtc cgccgtatga
     5461 caacgccagc ccgttccgca gcctgctctg gacgctggtc aacgccgaca agaacatgca
     5521 gtcgacgatt ctgagccgcg acttcttcgc cgccttctgc gtgttgtatc tgccgttcgc
     5581 cctgctgttg agcctggacc ccggtccgcg caaccgcctg ttgtgcgcgg cgtcggctgc
     5641 ctggccgcct tgctgacgat cctggccatc tt
//
LOCUS       DAOPCO010000257         5638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12387_length_5638_cov_3.960953, whole genome shotgun sequence.
ACCESSION   DAOPCO010000257 DAOPCO010000000
VERSION     DAOPCO010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12387_length_5638_cov_3.960953"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..502
                     /locus_tag="PLS90_14180"
     CDS             92..502
                     /locus_tag="PLS90_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HPK03591.1"
                     /translation="MRRMGLLVMTVVSVALLGGCAFRPGWFPAQSRMMGSLGQSLPVV
                     PVQPPPGFIYEQVSAPLSYNFAETPAQPGKTGVSQANYLCIPFIPYLSFSWGDVSIEN
                     AAREGNLDEVAYADYEVMQVLGVFTQMKIIAHGR"
     gene            799..2523
                     /locus_tag="PLS90_14185"
     CDS             799..2523
                     /locus_tag="PLS90_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03592.1"
                     /translation="MLLAAAALLLLPIAPLSADDRLAPAVLLILAAAGLFWMALDRRG
                     KARSRVIWSLLAILVGMLRVGYEYDRHNYVLYHSLIEHARDPSFLATDWFVRASAGFN
                     GYYFFVRACALLPPALLPPLFFGLWLLGFAALALLFRLIAHRIIPRFARAELCAAAAY
                     LFVLLTYAPGRWSLVALGDHGFFYPFVNPQVAGMLLGLLAAFLIAAGRPLAGSLPLGL
                     AINLHLSSGQHFFILCAVLVLLARPLQLPLSLPRGLAALALALLIGLPNLLPTLRNQL
                     AAPPTPETDIPYILIFGYFRHPHHTIPSTWPLKRFICFGIVLALGAAGWLLKRPHGPL
                     DRAHGAIVATCLLLMFAGWFFVERTPIDFVGKSQFFRLSVVAQILCAPYIAWLLARPL
                     PLPRNIASLDSTRLKRHAWALAATIALVAAGVALGRPFHLVARPGVPVEQWVAKNTPP
                     DAVFILPIRTYGIGLNEFPMRTGRGLVADLHRFPHQGVDYLTWFKRLCDLFGVECELN
                     PKAVRALGRANLTRMSQQLSLDQLRQLRDRYGATHLVRQVQYPLAGRDPVYRDQEFLV
                     YDLRPAAE"
     gene            complement(2555..3397)
                     /locus_tag="PLS90_14190"
     CDS             complement(2555..3397)
                     /locus_tag="PLS90_14190"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPK03593.1"
                     /translation="MLIARVISAIIFFAFFFLGLFNPLFVWALPLLLAVAALSALKEF
                     AHFTPQRPRPSFVWLAYGAALLMLADAYYYELDHALAILGLLTVISLGWGTLSLERDS
                     TSMAGVCLIGTLYVTLPLALITIIWRTAVQSNNQDGQHYIIFLVLGTQASDVGAYFIG
                     RLLGRHKMAPRISPGKSWEGFAGGVAFTLLLAVLAKLFWNNIDRLFGWYEIVGFALVF
                     SMVGPVGDLAESWLKRTAGIKDSGRTFTGHGGVLDIIDSLLFTTIIYYIYLGLSPRHE
                     IGIF"
     gene            complement(3411..4214)
                     /locus_tag="PLS90_14195"
     CDS             complement(3411..4214)
                     /locus_tag="PLS90_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPK03594.1"
                     /translation="MTMAQSWWPKAAERWSESERRLLGLLDPSRLPAHVAVIMDGNGR
                     WARQRGLISRIKGHEAGIESVREATRTCAELGLRVLTLYAFSKENWARPSAEVSALMN
                     LLSRFLVAERDELMENNIRLRMIGAREDLPDSARRALDETMALTAGNDGMTLNLALSY
                     GARDELARATRRIAEAVERGELRPEQITPDVIAQHLDTADLPDPDLLIRTSGELRVSN
                     FLLWQIAYAEIHVTPVLWPDFRREQLLSALVDFQGRERRYGTVRDLNES"
     gene            complement(4383..>5638)
                     /locus_tag="PLS90_14200"
     CDS             complement(4383..>5638)
                     /locus_tag="PLS90_14200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF036802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPK03595.1"
                     /translation="PHSFPDAMALVIETPVGALLHTGDFKFEPINGGRLPASLRAILR
                     HLKGRRPLLLMADSTNIDRDGHSPNETQVREGLTRFFRDAPRTIILTSFSTNIDRIQT
                     ILDLARQFGRKVAICGFSLDRNFNIAGELGLLQYPAGLVLPLHELRQVPPESRVIITT
                     GSQGEPLSALSRMALNSFRGYTVQPEDLIIFSSRIIPGNERSIYKLINHFYRHGAKVV
                     TERDGAVHASGHAYRDEMRLLLREVRPRFFMPIHGELRQLMRHGELAIECGLKEEEVF
                     ILENGMQLNLGEDWAEAQPTDWAGIVLVDGRMMDGVEQIVLRDRKHLAEDGMLTVILV
                     IDKQSHRIMAGPDLVSRGFIVVDNNEPLMQGCKDLVARTFEECPKESQEEWDTVKAAV
                     RKALRRYLADQTDRYPVILPVVVEI"
BASE COUNT          858 a         2189 c         1536 g         1055 t
ORIGIN      
        1 ttcggcgtct atcagcgcta ccgcacggtg gtctatggcg actgatcgcc ctctcccgcc
       61 gcgctcctac cttttgcaca ggaggtcgac catgcgccgc atgggtttac tcgtcatgac
      121 ggtcgtgtcg gtcgcgctgc ttggtggctg tgcctttcgg cccggatggt tccccgcgca
      181 gagccgcatg atgggctcgc tcggccagag cctcccggtc gtcccggttc agccccctcc
      241 gggcttcatc tacgaacagg tctcggcccc cctctcctac aacttcgccg agacccccgc
      301 ccaacccggc aaaacgggcg tgtcgcaggc caattacctg tgcatcccgt tcatccccta
      361 tctctccttc tcctgggggg atgtcagcat cgaaaatgcc gcccgcgagg gcaacctgga
      421 tgaagtggcc tacgccgact atgaggtgat gcaggtcctg ggggtcttca cccagatgaa
      481 gatcatcgcc cacggccgtt gacctttcac cacatcgcga accatcgcct gcgccccccg
      541 ggctcgcccc ggggggcgtt gctttttttc tcgtccatca tcttcgtcct cgtccctcgt
      601 cttcgtagtc gcccagaacg gcccccccaa gattcggctc tcggctctcg tcccccgtcc
      661 tcggctctcg aaaccccctc gtcctcctga tccctccctt tccccggttc actttcagcc
      721 gccggctcgt tatcctcgcc gccgatggcc cctttcccgc caatccctcc cgctttccgc
      781 cacttcgcgc ccgcaggttt gctcctggcc gcggccgcgc tgcttctgct cccgatcgcc
      841 ccgctctccg ccgacgatcg cctcgccccg gccgtcctgc tcatccttgc cgccgccggc
      901 ctgttctgga tggcactcga tcgccgcggc aaagcccgct cccgcgtcat ctggtcgctg
      961 ctcgccatcc tcgtcggcat gctgcgcgtc ggttacgaat acgaccgtca caactacgtc
     1021 ctctatcatt ccctgatcga gcacgcacgc gacccttcat tcctcgctac cgactggttc
     1081 gtccgcgcca gcgccgggtt caacggctat tatttcttcg tcagggcctg cgcgctcctc
     1141 ccgccggccc tgctcccgcc cctgttcttc gggctctggc tgctcggttt cgccgcgctg
     1201 gcgttgctct tccgcctcat cgcccaccgg atcatccccc gtttcgcgcg ggccgagctc
     1261 tgcgccgccg ccgcgtatct gttcgtcctt ctcacctacg cccccggccg ctggagtctg
     1321 gtcgccctcg gcgatcacgg cttcttttac ccgttcgtca acccgcaggt ggcgggcatg
     1381 ctgctcggcc tgctggccgc gttcctgatc gccgccgggc ggccgctcgc cgggtccctt
     1441 ccgctcggcc tggcgatcaa cctgcacctc agttcgggcc agcacttttt catcctttgc
     1501 gcggtcctcg tgctcctggc ccgtcccctc cagctcccgc tcagcctgcc gcgcggactc
     1561 gccgcccttg ccctcgccct cctgatcggc ctgcccaacc tcctcccgac gctgcgcaac
     1621 cagctcgcgg ccccgccgac gcccgaaacc gacatcccct acattctgat cttcggctac
     1681 ttccgccacc cgcatcatac gatcccctcc acctggccgc tcaagcgctt catctgcttc
     1741 ggcattgtcc tcgcgctggg cgccgccggc tggctgctta agcgtccgca cgggccgctc
     1801 gaccgcgccc acggcgcgat cgtcgcaacc tgcctgctgc tgatgttcgc cggctggttc
     1861 ttcgtcgaac gcaccccgat cgacttcgtc ggcaaatccc agttcttccg gctcagcgtc
     1921 gtcgcccaga ttctctgcgc gccctacatc gcctggctgc tcgcccgccc gctgccgctg
     1981 ccccggaaca tcgcatcact tgattcaacg cgcctgaagc ggcacgcctg ggccctcgcg
     2041 gcgacaatcg cccttgtcgc ggccggcgtg gccctcgggc gaccgttcca cctggtcgcc
     2101 cgccccggcg tccccgtgga gcagtgggtg gccaaaaaca cgccgcccga cgccgtcttt
     2161 attctcccca tccgcaccta tggcattggc ctcaatgaat tccccatgcg gacgggccgc
     2221 ggcctcgtcg ccgacctgca ccggttcccc catcagggcg ttgattatct gacctggttc
     2281 aagcgtctgt gcgatctgtt cggcgtggag tgcgaactga atccgaaggc cgtgcgcgcg
     2341 ctgggccgcg ccaacctgac ccgcatgtcg cagcagcttt ccctcgatca actccgccaa
     2401 ttgcgcgacc gctacggtgc aacacacctc gtccgccagg ttcagtatcc gctcgccggc
     2461 cgcgacccgg tctatcgcga ccaggagttt ctggtctacg atctgcgccc cgccgccgag
     2521 tagcgcgcga ataccggcgc cgccgctccc ctgctcaaaa aatcccgatc tcgtggcgcg
     2581 gtgaaagccc aaggtagata tagtagatga tcgtcgtgaa cagcagactg tcgatgatgt
     2641 ccagcacgcc gccgtggccg gtgaacgtcc gccccgagtc cttgatcccc gccgtccgct
     2701 tcagccagct ttcggccagg tcgcccaccg gccccaccat gctgaacacg agcgcgaacc
     2761 ccacgatttc ataccacccg aacaaccggt caatattgtt ccagaagagc ttggccagca
     2821 ccgccagcag cagcgtgaac gccacccccc cggcgaaccc ctcccacgac ttgccgggac
     2881 tgatccgcgg cgccatctta tgccgcccca gcagccgccc gatgaaatac gctcccacgt
     2941 ccgacgcctg cgtccccagc accagaaaaa taatgtagtg ctggccgtcc tggttgttgc
     3001 tctgcaccgc cgtccgccag atgatcgtga tcagcgccag cggcagcgtc acgtagagcg
     3061 tgccgatcag gcagaccccc gccatgctcg tcgagtcgcg ctcgagcgac agcgtccccc
     3121 accccagact gatcacggtc aacaagccca ggatcgccag cgcgtgatcc agttcataat
     3181 aataggcgtc ggccagcatc agcagcgcgg cgccgtacgc cagccagacg aacgacggac
     3241 gtggacgctg cggggtaaaa tgcgcaaact ccttcaacgc cgacagcgcc gccaccgcca
     3301 gcagcagcgg cagcgcccag acgaacagcg gattgaacaa ccccagaaaa aagaacgcga
     3361 aaaagatgat ggcggagatg acgcgggcga tcagcatcgg ctcggcgcgc tcagctttcg
     3421 ttcaggtcgc gtacggtccc gtagcgtctt tcccgcccct gaaagtccac cagggcgctc
     3481 aataattgtt cgcggcggaa gtcgggccac agcaccggcg tcacgtggat ctcggcatac
     3541 gcgatctgcc acagcagaaa gttgctcacc cgcagctcgc cgctcgtccg gatcagcaga
     3601 tccgggtcgg gcaggtcagc cgtgtccaga tgctgggcga tgacgtccgg cgtgatctgc
     3661 tcgggccgca gctcgccgcg ctcgacggct tcggcgatgc gccgcgtcgc ccgcgccagt
     3721 tcatcccgcg ccccgtagct cagcgccagg ttcagcgtca tcccgtcgtt gcccgcggtc
     3781 agcgccatcg tttcatccag cgcccgccgc gccgagtcgg gcaggtcctc gcgcgcgccg
     3841 atcatccgca gccggatgtt gttctccatc agctcgtcgc gctcggccac gagaaaccgc
     3901 gacagcaggt tcatcagggc gctcacctcc gcgctcggcc gcgcccagtt ctccttcgaa
     3961 aacgcataca gcgtcagcac ccgcagcccc agttcggcgc acgtccgcgt cgcctcgcgc
     4021 accgattcga tccccgcctc gtgccccttg atccggctga tcaggccgcg ctggcgcgcc
     4081 cagcgcccgt tgccgtccat gatcaccgcc acgtgcgccg gcagccgcga cggatccagc
     4141 aatcccagca gccgccgctc gctttcgctc cagcgctccg ccgccttggg ccaccagctt
     4201 tgggccatcg tcatcgtggt catcgcgatt actttccgcg ggctgcatcc atcctcgctc
     4261 atattgcccc gctccgcccg gaacatcaat gctttaacgg gcccgcgccc ccgcgcggtc
     4321 gcggcgtggc acacgcccat ccgtcccctt cattccccgc gaaacttcct tcgccggcgg
     4381 gcctagatct ccaccaccac cggcaggatg accggatagc gatcggtctg atcggccaga
     4441 taccgccgca gcgccttgcg caccgccgcc ttcaccgtgt cccattcctc ctggctttcc
     4501 ttggggcatt cctcgaacgt gcgcgccacc agatccttgc acccctgcat cagcggctcg
     4561 ttgttgtcca ccacgatgaa tccccgcgaa accaggtcgg gacccgccat gattcggtgc
     4621 gattgcttgt cgatcaccag gatcaccgtc agcatcccgt cctcggccag gtgcttccgg
     4681 tcgcgcagca cgatctgctc cacgccgtcc atcatccgcc cgtccacgag cacgatcccg
     4741 gcccagtcgg tcggctgcgc ttcggcccag tcctcgccca ggttgagctg catgccgttc
     4801 tccaggatga aaacctcctc ctccttcagc ccgcactcga tcgccagttc gccgtggcgc
     4861 atgagctggc gcagctcgcc atgaatcggc atgaagaacc gcggccgaac ctccctgagc
     4921 agcagccgca tctcatcgcg gtaggcgtgc cccgaagcgt gcaccgcccc gtcgcgttcg
     4981 gtcaccacct tcgccccgtg ccgatagaaa tggttgatca gcttgtaaat cgagcgctca
     5041 ttgcccggaa tgatccgcga gctgaaaatg atcaggtctt cgggctgcac cgtgtagccc
     5101 cgaaaactgt tgagcgccat ccgcgagagc gccgacagcg gctcgccctg cgagcccgtc
     5161 gtgataatca cgcgcgactc gggcggaacc tgccgcagct cgtgcaacgg aagcaccagc
     5221 cccgccgggt attgcagcag ccccagctcg ccggcgatgt tgaagttgcg gtccaggctg
     5281 aagccgcaga tcgccacctt gcgcccgaac tggcgcgcca ggtccagaat cgtctggatc
     5341 cggtcgatgt tggtcgaaaa cgacgtcagg atgatcgtcc gcggtgcgtc gcgaaaaaat
     5401 cgcgtcaacc cctcgcgcac ctgtgtctcg ttgggcgaat gcccgtcccg gtcgatattc
     5461 gtgctgtcgg ccatcagcag cagcggccgc cgccccttca aatgcctcag gatcgcgcgc
     5521 agcgacgcgg gcaaccgccc gccgttgatc ggctcaaatt tgaaatcgcc cgtgtgaagc
     5581 aacgccccca ccggcgtctc gatcaccagc gccatcgcgt ccggaaacga atgcggca
//
LOCUS       DAOPCO010000258         5637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12391_length_5637_cov_5.363669, whole genome shotgun sequence.
ACCESSION   DAOPCO010000258 DAOPCO010000000
VERSION     DAOPCO010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12391_length_5637_cov_5.363669"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLS90_14205"
     CDS             complement(<1..746)
                     /locus_tag="PLS90_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPK03596.1"
                     /translation="MLSPRTQRISFCLGLLLLAAAPLTAQPGGNLRFEPGAVESANAA
                     SPTAPLGREADAPQIHAEKTHLDLGTVNEGEKVEFAFPIVNLGRDTLRILQVQKSCGC
                     TEATAEKHILQPGESTQITGQFDSHGRRGMQHKIITVSSNDPKNPSLQLTFNVNVSQT
                     VLVEPERVSWGRISVEEATTQTVTIRSASAEPLEIKEVTVDGPAAERIWARLIDVRPT
                     PAVAGQLDGETSTTTEARIAVGIKPGGETGT"
     gene            complement(999..2108)
                     /gene="glf"
                     /locus_tag="PLS90_14210"
     CDS             complement(999..2108)
                     /gene="glf"
                     /locus_tag="PLS90_14210"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HPK03597.1"
                     /translation="MPLDYLIVGAGLFGSVFAQQMHEAGRSVLVIDRRPHLGGNCYSE
                     DHPGTNITVHRYGTHIFHTNNDHVWRYINRFTEFNRYQHRVLTTHRDRVYSLPINLAT
                     INQFYGVNLKPSEVEAFLAPRREGITQPRNLEEKAISLVGRDLYEAFIKGYTIKQWDR
                     DPRELPAAIVARLPWRTSYDDAYFDDRYQGIPVGGYTPIFERMLEGIDVELGVDFFDN
                     RAAWEARARRIVYTGPIDRFHGYRFGRLAWRSVRFEVEQLDCDDFQGTSVMNYADVET
                     PWTRIHEPKHLHREKTHRPAGTTVIMREYSQVDPEAPYYPVNTEADRRMLARYEELNA
                     ATQGVIFGGRLAEYKYYDMHQVIAGALARARRELT"
     gene            complement(2279..4486)
                     /locus_tag="PLS90_14215"
     CDS             complement(2279..4486)
                     /locus_tag="PLS90_14215"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPK03598.1"
                     /translation="MRKIVKHPARDLGYGLTGPMYLPPSEQERALRDALQPGLHTYRP
                     LKSGEIDALQQNGCSSDDWSSVQVGDRFNPRQVVGCRFCGLVRIGNLGNLVHEVAGLK
                     LPVGLYHSTIVACDIGENPVIHHARYLAYYLLGDDVLLHNVGRMAVGPDARFGHGILK
                     GEEPEEARSWIDLGNENGRRRILAFDGILPADAWLWSRHRDDAQLLERFKELTDARFD
                     RRPGYYGTVGDRTAIINTGSVIDTAIGTDATIDGASRLENLTIRSTADEPVTIGDDVT
                     LRDGIVGAGCTIAGGVRAERFVFGSSVRLDQGARLRHCFIADNAHIACAELASVLTYP
                     HHQQHHNNSFLIAATLLGQSNIAAGATIGSNHNSRGADWEFLAGRGFFPGLCTSFRYP
                     SRFASFVMVVKGDYPAELNIPFPFSLVARGDDGELRVTPAFALLENAYMLARQERNGR
                     RRDQRVTRRQHIVTDVLAPDTADEMLRGAALIEQAVGRAVLEQGEIPPDEAPAEDLAL
                     IGRKLLAEEAEAVERLELRLAGLERSRRPARLLKAAAAWRAYRDALEFYAMRTLLEAM
                     RSRHMDGPAELHEWFGVGNGAPGWVNLGGQLVPAPEVEILRARLRAGEFTRWEEVHEE
                     YDRLWDQYPEDVARHAYQILRGLYEVEGDGLPAEPWRRALARAAELSQAWVERVVRAR
                     SRDYDDPFRRMTCDSAAEWEAVMGSLAEDAVIQETQEAHNRFKDLVAHYQSTA"
BASE COUNT          857 a         1881 c         1970 g          929 t
ORIGIN      
        1 gtcccggtct cgccccccgg cttgattccg acggcgatcc gcgcctcggt cgtcgtgctc
       61 gtttcgccgt ccaactgccc cgccaccgcc ggcgtcggcc ggacgtcgat cagccgcgcc
      121 cagatccgct cggccgccgg cccgtccacc gtgacttcct tgatctccag cggctcggcg
      181 ctggccgatc ggatcgtcac cgtctgcgtc gtcgcttcct ccaccgagat tcgcccccac
      241 gacacccgct ccggctccac cagcaccgtc tggctgacgt tcacattgaa cgtgagctgc
      301 aggctcggat tcttcgggtc gttcgacgac accgtgatga tcttgtgctg catcccccgc
      361 cggccgtggc tgtcgaattg ccccgtgatc tgggtgctct cgcccggctg caggatgtgt
      421 ttttcggccg tcgcctcggt gcagccgcac gacttctgca cctgcaggat gcgcagcgtg
      481 tcgcgcccca gattcacgat cggaaacgcg aattccacct tctccccctc attgaccgtc
      541 cccagatcca gatgcgtctt ctccgcgtgg atctgcggcg cgtccgcctc gcggcccagc
      601 ggcgccgtgg gcgacgccgc attggccgac tccaccgcac ccggttcaaa ccgcagattg
      661 ccgccgggct gggcggtcag cggcgccgcc gcaagcagca gcagccccag gcagaaactg
      721 attcgttggg ttctcgggga gagcatcggt cgatcttcct gaacaatatg ggagcgcacg
      781 gccccgccgc ggcgacgcct gccgtcaagg accgcttgcc ttccattttg ttccatccgc
      841 cggcattgtg caagccacag gccctcccat aattcgtctc tcggcgtcgt gatcgaccct
      901 gtggccaagg ctccgcctcc agggtgacgg ccgcaggccg tcacactgcg tcaccgcgca
      961 tacccggacc gcccaggtgg caagatcgcc cagtccgctc acgtcagctc gcgccgcgcg
     1021 cgcgccagcg cgcccgcaat cacctggtgc atgtcgtagt acttgtattc ggccagccgc
     1081 ccgccgaaga tcaccccctg tgtcgccgcg ttgagttcct cgtagcgcgc cagcatccgc
     1141 cggtccgcct cggtgttgac cgggtaatac ggcgcttccg ggtcgacctg cgaatactcg
     1201 cgcatgatca cggtcgtccc cgccggccgg tgcgtcttct cgcggtgcag gtgcttcggt
     1261 tcatggatcc gcgtccacgg cgtctccacg tcggcgtagt tcatcacgct cgtcccctgg
     1321 aagtcgtcgc agtcgagctg ctcgacctcg aaccgcacgc tgcgccacgc cagccggccg
     1381 aaacggtaac catgaaaccg gtcgatcggt ccggtgtaga cgatccgccg cgcgcgcgcc
     1441 tcccatgccg cgcggttgtc gaaaaagtcg actcccagct ccacgtcgat cccctccagc
     1501 atccgctcga agatcggcgt gtagccgccc accgggatcc cctggtagcg gtcgtcgaag
     1561 taggcgtcgt cgtaggaggt gcgccacggc agccgcgcga cgatcgcggc gggcagctcg
     1621 cgcgggtcgc ggtcccactg cttgatcgtg tagcccttga tgaacgcctc atacaggtcg
     1681 cggccgacca gactgatcgc cttctcctcc agattgcgcg gctgcgtgat cccctcgcgc
     1741 cgcggcgcca gaaacgcttc cacctccgac ggcttcaggt tgacgccgta gaactggttg
     1801 atcgtcgcca ggttgatcgg cagcgaatac acccggtcgc ggtgcgtcgt cagcacgcgg
     1861 tgctggtagc ggttgaactc ggtgaagcgg ttgatgtaac gccacacgtg atcgttgttg
     1921 gtgtgaaaga tgtgcgtgcc gtagcggtgg acggtgatgt tcgtcccggg gtggtcctcg
     1981 ctgtagcagt tgccgccaag gtgcgggcgc cggtcgatca ccagcacgct gcggcccgcc
     2041 tcgtgcatct gctgcgcgaa taccgaccca aacagccccg cgcccacgat gagatagtcc
     2101 aacggcatgg cagaccccga ctgcaaactt ccggcgacgc ccgccgccgg gcgccctacc
     2161 ttagcacctt cccttcgaaa ggacgaccga ttaggcgacc cgcggttctg gccgctccgt
     2221 gttccgcgat tcgattgacg agggcgatcc cgataccgct tcttcgcgct tcccgtcctc
     2281 aggcggtcga ctggtagtgc gccaccaggt ccttgaaccg gttgtgcgcc tcctgggtct
     2341 cctgaatcac ggcgtcctcg gccagcgaac ccatcaccgc ctcccactcc gcggccgaat
     2401 cgcaggtcat ccggcggaac gggtcatcgt aatcgcgcga gcgcgcacgg acgacgcgct
     2461 cgacccacgc ctggctgagc tccgcggcgc gcgccagcgc gcgccgccac ggctcggcgg
     2521 gcagcccgtc gccctccacc tcgtacaggc cgcgcaggat ctggtaggca tggcgcgcca
     2581 cgtcctccgg atactggtcc cacagccggt cgtattcctc gtgaacttcc tcccagcggg
     2641 tgaattcccc ggcgcggagg cgcgcgcgca gaatctccac ctccggcgcc gggacgagct
     2701 ggccgcccag gttgacccac cccggcgcgc cgttgccgac gccgaaccat tcgtgcagtt
     2761 cggcggggcc gtccatgtgg cgcgagcgca tcgcctccag cagcgtccgc atcgcgtaga
     2821 attccagcgc gtcgcgatag gcgcgccacg cggccgccgc cttgagcagc cgcgccggcc
     2881 gccgcgaccg ctccagcccc gccagccgca gctccagccg ctcgaccgct tcggcttcct
     2941 ccgccagcag tttgcgcccg atcagcgcca ggtcctccgc gggcgcttcg tccggcggga
     3001 tctccccctg ctccagcacg gcgcggccca ccgcctgctc gatcaacgcc gccccgcgca
     3061 gcatctcgtc ggccgtgtcg ggcgccagca cgtcggtcac gatgtgctgg cggcgggtca
     3121 cgcgctggtc gcggcgccgg ccattgcgct cctgtcgcgc cagcatgtag gcgttctcca
     3181 gcagcgcgaa cgccggcgtc acccgcagct ccccgtcgtc gccgcgcgca accaggctga
     3241 agggaaacgg gatgttcagc tccgccgggt agtccccctt gacgaccatc acgaacgagg
     3301 cgaaccgcga cggatagcgg aagctcgtgc acaggcccgg aaagaagccg cgcccggcga
     3361 ggaactccca gtccgcgccg cgcgagttgt ggttggagcc gatcgtcgcc cccgccgcga
     3421 tgttgctctg gccgagcagc gtcgccgcga tcaggaacga attgttgtgg tgctgctggt
     3481 gatgcgggta ggtcaggacg ctggccagct cggcgcaggc gatatgcgcg ttgtcggcga
     3541 tgaagcagtg gcgcagccgc gccccctggt ccaggcgcac ggaggaaccg aagacgaagc
     3601 gctcggcgcg gacgcccccg gcgatcgtgc agcccgcgcc gacgatgccg tcgcgcagcg
     3661 tcacgtcgtc gccgatcgtc accggctcgt cggccgtgct gcggatcgtc aggttctcca
     3721 gccggctggc gccgtcgatc gtcgcgtcgg tcccgatcgc ggtgtcgatc acgctgccgg
     3781 tgttgatgat cgccgtccgg tcgcccaccg tgccgtagta acccggccgg cggtcgaagc
     3841 gcgcgtcggt cagctccttg aagcgctcga ggagttgcgc gtcgtcgcgg tggcgcgacc
     3901 agagccatgc gtcggccggc aggatgccgt cgaacgccag gatccgccgc cggccgttct
     3961 cgttgcccaa gtcgatccac gagcgcgcct cctccggctc ctcccctttc aggatgccat
     4021 ggccgaaccg cgcgtcgggg ccgacggcca tccgccccac gttgtgcagc agcacgtcgt
     4081 cgccgagcag gtaatacgcc agatagcgcg cgtggtgaat caccgggttc tcgccgatgt
     4141 cgcacgccac gatcgtgctg tgatacagcc ccaccggcag tttgagcccc gcgacctcgt
     4201 gcaccaggtt gcccaggttg ccgatccgca ccaggccgca gaaccggcag ccgaccacct
     4261 ggcgcggatt gaaccggtcg ccgacctgca cgctcgacca gtcgtccgag ctgcagccgt
     4321 tctgttgcag cgcatcgatt tcgcccgatt tgagcgggcg ataggtgtgc agccccggct
     4381 gcagcgcgtc gcgcagcgcg cgttcctgtt cgctgggggg cagatacatc ggcccggtca
     4441 gcccgtagcc caggtcgcgc gccggatgct tgacgatctt ccgcatgatc gcttccctcc
     4501 ccatgttcca accgccctgg ctccgtctgg tgtagcaaac tcgcgcccga agcgaaagcc
     4561 ccgccgcgcg gggaaggggg ggatgggcga tctgtatttg accaggggca acggcgagag
     4621 gccgagaccg cgatggattt cggcatcagg gggaaagggc gtggagcggg gagggagtaa
     4681 ggggatcagg atttcggggg cggatgccag tcggaaaggc cggcggggag ggaagcgtgg
     4741 ggagagacgg atgaggggga ttgggagaga gtggggatgg gggcggacga gatgggcacg
     4801 gtggacttga tggagcttat gaaggggcag gacgaggtgg acaaagtgga tgaggtgatt
     4861 ggggaatgcg tgggcggcgt ggggtcagtc ggcggcatgg ggcgatcagg agattggagc
     4921 tcccgaggga gagctgggat gaaagggcac ggggggatga gacgaatcag ggggaggagg
     4981 ggattgagcg ggaggaggtc ttccatcgca acgggcttgg gtgcggagga tgccatgggg
     5041 tgggattggc gaggggcgga gaggatgaag gaaagggggc ggagggggct cggatttttg
     5101 caggacgccc cagaagggat gaccggcaag ggcgccggtc ccccgctggc atggggggat
     5161 tggaaatccg acggatcgga ccgatcggac cgatcggacg gaatttggat gggggagacg
     5221 gcggagggaa tgaccggcgg gggaggcggt ccctcactgg aatttggcga tggcgatggc
     5281 gatggcgatg gtgatcccga tggggttggg gagaccggcg ggggcgccgg tcccacagtg
     5341 ggggtgaggt tgagggcgag gaaggtgcgg cggacagcga cggctttggc gagggagcga
     5401 gcgatgaggt tgacgcggac ttcggtgggg agaggtctgg ggaggtcgta gccgggtttg
     5461 aggccgtagt aattggcgtc ggcggcgcag agttcggggt gcggccgggg ggcatggatc
     5521 ggggagagct cgccggggag gtcggcgggg gtgagatcga ggtcgccggc gaggcgaaca
     5581 atcgagcgca ggaggacggc ttcgaaggcg tcgatggcgt gggagagggt ggcgagg
//
LOCUS       DAOPCO010000259         5577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12518_length_5577_cov_4.071713, whole genome shotgun sequence.
ACCESSION   DAOPCO010000259 DAOPCO010000000
VERSION     DAOPCO010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12518_length_5577_cov_4.071713"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..1694
                     /locus_tag="PLS90_14220"
     CDS             516..1694
                     /locus_tag="PLS90_14220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK03599.1"
                     /translation="MPRRPRTVLIVTPSSRLLGARRSLLALVEALDEDRWRPVVCGQS
                     VGQLGRALESLAIPMEVVRLGWWRKGKYFLWRPFAIARLAALARSVNADLIHCNEIYS
                     NPYAVRAARNVPAPADSPCAGGAVPVLTHVRLGMKRGMIRKYDLGRADGIIAPSRAVA
                     REFDGWEEPAPRVHVVHNGVNMDEFHRTLSPDAARDRIGVPRDGLLLGAIGQLGPRKG
                     GDLILDAFARLAPRHPNLRLIFVGDPHRGQEQFADDLKRRAAEPPLAGRVFFFPFTDQ
                     IIPYYEAIDVNLLISRKEGFGRTIIEASALGIPSIGAATGGIEEIIADGITGRLVPPD
                     DPVALADAIGQLAANDSLRRMMADAAFRHCAQHFSIAAHARRIMDIYDETLEARGANL
                     "
     gene            1809..2795
                     /locus_tag="PLS90_14225"
     CDS             1809..2795
                     /locus_tag="PLS90_14225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK03600.1"
                     /translation="MIRPVTPDDLPAIRDLFARYPYKHTQQKVQKLDRDRLALFYQRQ
                     LETALARGARAWLALDRDRPSALAGLALNAWHGQVYGRVMARLQPWLNTEMPHAGPPL
                     LAAVRAAARADGIDHLAVRLDGEDFLNLHLFEADGWRLVDLSMKFSRELPPGEPPPPP
                     PPGSFHIEPAAAADHPWIRRLGSTTHGATHFLNDPALPRERTVELFSRWIDRCLAGLA
                     WRVFLLRERPGGPGLGFVIFLRNPALAEAVGRRPLILDFVLLDPAARGRGLGAWFIQE
                     TVRRAADEARRAGEPFDFCELRTSAHNLPAVVAYEKAGFLCCATDMVLHGPG"
     gene            2956..4317
                     /locus_tag="PLS90_14230"
     CDS             2956..4317
                     /locus_tag="PLS90_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03601.1"
                     /translation="MGFFDSKSRQLRRKLKDLQAELKDAEKDGAYLLEGGVFFAEMDT
                     MGEATMMVFSTAQQHGADEEEIIETFFDRNGFTVEQLALNKQRDRIEQIEIIPPNELL
                     MAVAKALQSEGFRVVDDSAQERPVDLEHKLFPITALARATSLLFAQRVHQIIRSTRVK
                     MTKEGNRLLSWMPLVVEALRNRVDLLGADALKPLFDQTFELFFVQPFEKPSGKRSHTP
                     PPPPARPASMPAPARAAAPTDSQPLRAPRPAPARPVASPAAAVMAPPAPTPTPAAGPT
                     PQQIIARLTQSEQLAKVMAAMYRSLVKASPQRCVRNAESLLHHVNRLFNASATCLLLR
                     LPGRDQMSIYAQAGRKLEWGEGAGGEGFAISHGTVQDCLQKKTVVASAPLGGGDPSES
                     MVMYQIDATAAAPITLDDEIQGILYVDRRERALDFSREELDTLRKVSQVFQEFPDLTM
                     GLS"
     gene            4444..>5577
                     /locus_tag="PLS90_14235"
     CDS             4444..>5577
                     /locus_tag="PLS90_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPK03602.1"
                     /translation="MPPFRIHSEFQLQGDQPRAVDQLVAGLNAGLPAQVLLGVTGSGK
                     TFTMANIVERVQRPTLVLSHNKILAAQLYREFKEMFPDNAVEFFVSYYDYYQPEAYIA
                     RTDTYIEKDSSINEELDKMRLSATKSLIERRDVLIVSSVSCIYGIGNPGEYQKSVIII
                     ERGRTADRDEILRKLVDGLHVRSDVDFHRGTFRVRGDVVDVYPAYEDKSAIRIEFFGD
                     EVEAIHEIDPLTGQRRRGLQRAAIFPTSHYVTSPARLQAALANIAVELEERVAELEAQ
                     NKLVEAQRLRGRTRYDMEMMLEMGTCPGIENYSRHLDGRAPGEPPHTLLDFFPDDFLL
                     FIDESHVTIPQIRAMYNGDRSRKSVLVDFGFRLPSALDNRPLTF"
BASE COUNT          862 a         2404 c         1494 g          817 t
ORIGIN      
        1 ggcccgcccc aaaactcggc tcccggctct cgtccctcgt cctcggctct cgaaatcccg
       61 ccgaaacctg gaagcgccac gctcctgagt ggcctcgccc ccatcggccc gcccaaaaac
      121 tcggctctcg gctctcgtcc ctcgtcctcg gctctcgaaa accagccaaa acctgggagc
      181 gccacgctcc tgagtggcct cgcccccatc ggcccgccca aaaactcggc tctcggctct
      241 cgtccctcgt cctcggctct cgaaaaccag ccaaaacctg ggagcgccac gttcctgagt
      301 ggcctccccc ccaaggcccg ccccaaaact cggctctcga aaccccacca tcaccctggc
      361 ccacttcccc ctccccctcg cagcccgctt aaacgcttgc cgcaagcgaa gttgacaatt
      421 ccagggcaac ctgaaatcgt tcaggcattc cgcgatcgac gccccccgcg atcgctcctg
      481 ggggggcatt tcgacggggg atgaggatga cccttttgcc gcgcaggccc cgcacggtcc
      541 tgattgttac ccccagttcg cgtttgctcg gcgcgcggcg cagtctgctc gccttggtcg
      601 aggccctcga cgaggaccgc tggcggcccg tcgtctgcgg ccagagcgtc ggccagctcg
      661 gccgtgccct ggaatccctc gccatcccca tggaggtcgt ccgcctcggc tggtggcgca
      721 agggcaaata cttcctctgg cggcccttcg ccattgcccg cctggccgcc ctcgcccgct
      781 cggtcaacgc cgatctgatc cactgcaacg agatctactc caacccctat gccgtccgcg
      841 ccgcgcgcaa tgtccctgcc ccggccgatt ccccctgcgc cggcggggcc gtccccgtcc
      901 tcacccacgt ccgcctgggc atgaagcgcg gcatgatccg caagtatgat ctcggccgcg
      961 ccgacggcat cattgccccc tcgcgcgccg tcgcccgcga gttcgacggc tgggaggagc
     1021 ccgccccccg cgtccatgtc gtccataacg gcgtcaacat ggacgagttc catcgcacgc
     1081 tcagccccga cgccgcccgc gaccgcatcg gcgtccctcg cgacggcctg ctcctgggcg
     1141 ccatcggcca gctcggcccg cgcaagggcg gcgatctgat cctcgatgcc ttcgcccgcc
     1201 tcgccccgcg ccaccccaac ctccgcctga tcttcgtcgg cgacccccac cgcggccagg
     1261 agcagttcgc cgacgacctc aagcgccgcg ccgccgagcc ccccctcgcc ggccgcgtct
     1321 tcttcttccc cttcaccgac cagatcatcc cctactacga ggctatcgac gtcaaccttc
     1381 tcatctcgcg caaggaaggc ttcggccgca ccataatcga agccagcgcc ctgggcatcc
     1441 cctccatcgg cgccgccacc ggcggcatcg aggagatcat cgccgacggc atcaccggcc
     1501 gcctcgtccc ccccgacgac cccgtcgccc tggccgacgc catcggccag ttggccgcca
     1561 acgacagcct gcgccgcatg atggccgacg ccgccttccg ccactgcgcc cagcatttct
     1621 ccatcgccgc ccacgcccgg cgcatcatgg acatctatga cgaaacactt gaagccaggg
     1681 gcgccaacct ctgacacgcc atttgcttgc ataccgcccg ccggccgccc ccggcgcgcc
     1741 gtcccggccc gcgcgaacac ccccgcgccc cgcgccatcc cccgcgacct gacgccccga
     1801 cgccccggat gatccgccct gtcacccccg acgacctgcc cgccatccgc gatctcttcg
     1861 cgcgctaccc ctacaagcac acccagcaga aggtccagaa actcgaccgc gaccgcctcg
     1921 ccctgtttta tcagcgccag ctcgaaaccg ccctggcccg cggcgcccgc gcctggctcg
     1981 ccctggaccg cgaccgcccc agcgccttgg ccggcctcgc cctcaatgcc tggcacggcc
     2041 aggtttacgg ccgcgtcatg gcccgcctcc agccctggct caacaccgaa atgccccacg
     2101 ccggcccccc tctgctcgcg gccgtccgcg ccgccgcccg cgccgacggc atcgaccacc
     2161 tcgccgtccg cctcgacggc gaggacttcc tcaacctcca cctgttcgag gccgacggct
     2221 ggcgtctcgt cgacctctcg atgaagttct cccgcgaact cccccccggc gagcccccgc
     2281 ctcctccgcc ccccggctcg ttccacatcg agccggccgc cgccgccgac cacccctgga
     2341 tccgccggct cggttccacc acccacggcg ccacgcactt cctcaacgac ccggcgctcc
     2401 ctcgcgaacg caccgtcgaa ctcttctcgc gctggatcga ccgctgcctg gccggcctcg
     2461 cctggcgcgt cttcctcctg cgcgagcgac ccggcggccc cggcctgggc ttcgtcatct
     2521 tcctgcgcaa ccccgccctg gccgaggcgg tcggccgccg ccccctgatt ctcgatttcg
     2581 tcctgctcga ccccgccgcg cgcggccgcg gcctcggcgc ctggttcatc caggagaccg
     2641 tccgccgcgc cgccgacgag gcccgccgcg ccggcgaacc cttcgatttc tgtgagttgc
     2701 gcacctcggc ccacaacctc cccgccgtcg tcgcctacga aaaggccggc ttcctctgct
     2761 gcgccaccga catggttctg cacggccccg gttgaaacgc gccgccgttc ggcatattct
     2821 gaatgcgcgg acccggtccg caatgcgttc cagcctgaat cgatccgccg gcgcccgccg
     2881 cgtccggtcc gccgccccgt ccgccccccc cggcccggcg cggacgaaac cggccatccc
     2941 ccacggagca cgaccatggg attcttcgac agcaagagcc gccaactgcg ccgcaagctc
     3001 aaggacctgc aggccgagct caaggacgcc gaaaaggacg gcgcctatct cctcgagggg
     3061 ggcgtcttct tcgccgaaat ggacaccatg ggcgaggcca cgatgatggt cttctccacc
     3121 gctcagcagc acggcgccga cgaggaggag atcatcgaga ccttcttcga ccgcaacggc
     3181 ttcaccgtcg aacagctcgc cctcaacaag cagcgcgacc gcatcgagca gatcgaaatc
     3241 atccccccca acgaactgct gatggccgtc gccaaggccc tccagagcga aggcttccgc
     3301 gtcgtcgacg acagcgccca ggaacgcccc gtcgatctgg aacacaagct cttccccatc
     3361 accgccctcg cccgcgccac gtcgctcctc ttcgcccagc gcgtccacca gatcatccgc
     3421 tccacccgcg tcaagatgac caaggagggc aatcgcctcc tgagctggat gcccctggtc
     3481 gtcgaggcgc tgcgcaaccg ggtcgacctc ctcggcgccg acgccctcaa acccctcttc
     3541 gaccagacgt ttgaactgtt cttcgttcag cccttcgaaa aaccgtcggg caagcgctcg
     3601 cacacgccgc cgccgccccc cgcccggccg gcgtccatgc ccgcgcccgc gcgcgccgcc
     3661 gcccccaccg acagccagcc gctgcgcgcc ccgcggccgg cccccgcccg gccggtcgcc
     3721 tcacccgctg ccgccgtcat ggcccccccg gccccgacgc cgacccccgc cgccgggccc
     3781 accccccagc agatcatcgc ccgactcacc cagagcgagc agctcgccaa ggtgatggcc
     3841 gcgatgtatc gctcgctggt caaggccagc ccgcagcgct gcgtccgcaa cgccgagagc
     3901 ctgctgcacc acgtcaaccg cctgttcaac gcctcggcca cctgcctgct gctgcgcctc
     3961 cccggccgcg accagatgtc gatctacgcc caggccggcc ggaaactgga gtggggcgag
     4021 ggggccggcg gcgagggctt cgccatctcc cacggcaccg tccaggactg cctccagaag
     4081 aaaaccgtcg tcgccagcgc cccgctcggc ggcggcgacc ccagcgaaag catggtcatg
     4141 tatcagatcg acgccaccgc cgccgccccc atcacgctcg acgacgaaat ccagggcatc
     4201 ctgtatgtcg accgccgcga gcgcgcgctc gatttctcgc gcgaggagct cgacaccctg
     4261 cgcaaggtct cccaggtctt ccaggaattc cccgacctga cgatgggcct gagctgacgc
     4321 cgcccacgcc gccgatgccg cgcacgccgc cgacgccgct ggctcggccg gcccgcccgc
     4381 gctacaatgg ctccgctgag cgccgcggcg tcggcgcatt cctcgctcac ggtccgtcgc
     4441 gccttgcctc cgttccgcat tcactccgaa ttccagctcc agggcgatca gccccgcgcc
     4501 gtcgaccagc tcgtcgccgg cctcaacgcc ggcctgcccg cccaggtcct gctcggcgtc
     4561 accggctcgg gcaagacctt caccatggcc aacatcgtcg agcgcgtcca gcgccccacg
     4621 ctcgtcctct cccacaacaa gatcctcgcc gcccagctct accgcgaatt caaggagatg
     4681 ttccccgaca acgccgtcga gttcttcgtc tcctactacg actactacca gccggaggcc
     4741 tacatcgccc gcaccgacac ctacatcgag aaggacagct cgatcaacga ggagctggac
     4801 aagatgcgcc tgtcggccac caagagcctg atcgagcggc gcgacgtcct catcgtcagc
     4861 tccgtctcgt gcatctacgg catcggcaac cccggcgagt atcagaaatc ggtgatcatc
     4921 atcgagcgcg gccgcaccgc cgaccgcgac gagatcctgc gcaagctcgt cgacggcctc
     4981 cacgtccgct ccgacgtcga tttccaccgc ggcaccttcc gcgtccgcgg cgacgtcgtc
     5041 gacgtctacc ccgcctacga ggacaagtcc gccatccgca tcgagttctt cggcgacgag
     5101 gtcgaggcga tccacgagat cgaccccctc accggccagc gccgccgcgg cctccagcgc
     5161 gccgccatct tccccaccag ccactacgtc accagccccg cccggctgca ggccgcgctg
     5221 gccaacatcg ccgtcgaact cgaggaacgc gtcgccgaac tggaggccca gaacaaactc
     5281 gtcgaggccc agcgcctgcg cggccgcacc cgctacgata tggaaatgat gctcgaaatg
     5341 ggcacctgcc ccggcatcga aaactactcg cgccacctcg acggccgcgc ccccggcgag
     5401 cccccgcaca cgctcctcga cttcttcccc gacgacttcc tgctcttcat cgacgaaagc
     5461 cacgtcacca tcccgcagat ccgcgcgatg tataacggcg accgctcgcg caagtccgtc
     5521 ctggtcgatt tcggcttccg cctcccctcc gccctcgaca accgcccgct caccttc
//
LOCUS       DAOPCO010000260         5565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12542_length_5565_cov_4.896189, whole genome shotgun sequence.
ACCESSION   DAOPCO010000260 DAOPCO010000000
VERSION     DAOPCO010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12542_length_5565_cov_4.896189"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            538..1089
                     /locus_tag="PLS90_14240"
     CDS             538..1089
                     /locus_tag="PLS90_14240"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPK03603.1"
                     /translation="MPRVSCRESILAAAETVVIEAGAAHLTLEAVAERAGLSKGGLIY
                     HFPTKESLLEAMVARMAERFEQERERVRRQLGDRNASDLVVEILTLQGYSRDHHRLSA
                     ALLAVVANKPELIGGTRAELRKRFHRRLTSRGDFNPCAVLFFAALGLHFHDLLNLSLL
                     SRKQRGELYRHLVNEARKEVESP"
     gene            1182..2561
                     /locus_tag="PLS90_14245"
     CDS             1182..2561
                     /locus_tag="PLS90_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK03604.1"
                     /translation="MNSRRVRGAAAKTLVGWLLVVAAGAAIYHYTRPGTAEEAGGGGG
                     PEGAPPPEVAVEVVEPRPVTLKTELPGRTAARLIAEIRPQVSGLIQQRLFTEGSDVKQ
                     GEVLYIIDPAPFEAEVAQAEANLTGARQAADRARAAIAAAEAAVRQRQAVLTLARTDL
                     ERIEQLASEGIVSQSERDKMVTEARVAEAALVSAEAELASQREAVGVAEAAIGQAQAA
                     LEAARIQLDHTRIVAPISGRIGISQVTVGALVTAHQATPLATIQQLDPIYVDVSQSTS
                     ELLALRRRLESAELTSDTATIDTVGLRLEDGSAYDHTGTLAFRDVTVDQSTGSVTLRA
                     VFPNPEGLLLPGMFVHAIVTEGTRNDAILVSQQAVSRDPKGNPLALTVNAEGKVEQRM
                     LTLDRAIGDQWLVTSGLARGDRVIIEGMQRVRPGAAVVVASDAGGETAAETRVSDSSV
                     SSVYSGNGA"
     gene            2561..>5565
                     /locus_tag="PLS90_14250"
     CDS             2561..>5565
                     /locus_tag="PLS90_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK03605.1"
                     /translation="MLSSFFLKRPVFAWVIAIVLMLAGALAIYHLPVSQYPPIAPPSI
                     AIDAFYPGASAQTVEDSVVQIIEQKMTGFDNLLYTSATSDASGAGRVELTFAPGTDPD
                     LAWAQVQNKLQLAMASLPEVVQRAGVKVSKSTRNYLIIVGLVSEDGSMDSADLNDYAQ
                     SSIEKVLARVPGVGEVNTFGSQYAMRVWLNPEKLTQYQLTITDVVQALAKYNVEVSAG
                     QFGGAPAVEGQRLNASIIVQNLLQTPEEFAAIPLRTNSDGSVVRVSDVGRTELGTEFY
                     GSDALFNGKPSAALAIRQAAGANALDTADAVKKKMEELSAFFPPGMKVIYPYDTTPFV
                     KAAMEEVVKTLFEAIVLVFFVMYVFMGNLRATLIPTIAVPVVILGTFATLALFGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMTEEGLAPRAATEKSMREITSALIGIGLVL
                     SAVFGPMAFFGSSTGVIYRQFSVTIIAAMVLSVVVALILTPVLCVSLLKPVARHHSPG
                     EHAIFFMRPFFRWFERVFDRARVTYLAVVGRILVRKMRYIVIFLLIVGGMFYLFRRMP
                     TAYLPDEDQGILFVQATLPTGSTLEQTEAVMAEVREYFMEQEHEAAQSTLTIAGRSFS
                     GVAQNAGLAFVKLRDWDERHAPELKVKAVVNRAMDRFARIRNASVFAFPPPAVIELGQ
                     AGGFDFQLLDRGGVGHDALMEARNQILSMAAEEPRLTRVRANGLEDVPQYRVEVDWGK
                     AGALGVAIDAIHATISASFGSAYVNDFIQGGRVKRVYVQADAPYRMLPEDLKKLYVRN
                     PEGRMVPFASFATGHWTYGSPKLERFNGFPSINILGEPAPGRSSGEAMQLMEEFVKKL
                     PQGIAHDWTGLSYQERMAGSQAPILYAFSILVIFLCLAALYESWPVPVSVLLALPLGA
                     IGGVIASTLRDLPNDVYFQIGLLTTLGLTTKNAILIVQFAKARYDEGMDLVEATLEGA
                     KLRVRPIVMTSLAFGFGVIPLAFANGAGAG"
BASE COUNT          921 a         1557 c         2089 g          998 t
ORIGIN      
        1 ggatcgaaag ccgagagccg aatcctgggt ggcccgaggg ggggcgtgaa ggcgatggac
       61 gggagcgaga cggggggcct acgttaatta tggattctct gtctttctgg gggaggtcgg
      121 tgggatgtca ggtccaaacc ctggcctgtg tgggtcttgt gctacggaat tccctcgccg
      181 gataacgcgc gcaacatttc gctgtagtct tcgttcagca tcgaaaccct cgcagcgcga
      241 ccaggcctgg caggtcagcg gccagaccat ggcgaggcga ctggccatcg tccccgggac
      301 gtaatcatcc tcaaagccgc agtcagtgag ggtgtttccg ggtaaaccgg gtgcggtcca
      361 tcggggagat tgccgccgtg ctgttccatg gtcgcgtccc attcaccgct gatcgccgat
      421 ctgtgacaca gggccggtgg tttttcaatg gggggctcgg cgcgttgttg acatgttgcc
      481 gacagaggag gatactgtac cgtccggtcg gtacagtcag gaccggagga ggggccgatg
      541 ccgcgggtca gttgccggga atcgatactt gccgccgccg aaacggtcgt gattgaagcg
      601 ggggcggcgc atctgacgct ggaggccgtc gccgagcgcg ccgggctcag caagggcggg
      661 ctgatctacc attttccgac gaaggagtcg ctgctggagg cgatggtcgc ccggatggcg
      721 gagcgtttcg agcaggaacg cgaacgtgtc cgccggcagc ttggcgaccg gaacgccagc
      781 gatctggtcg tggagatcct gacactgcag ggctattcgc gcgatcacca tcgcctgagc
      841 gcggcgctgc tggcggtggt ggcgaacaag ccggaactga tcggcggcac gcgcgcggag
      901 ctgcgtaaac ggttccatcg gcggctgacg tcccggggcg atttcaatcc ctgcgcggtg
      961 ctcttcttcg cggcgctggg tctgcacttt cacgacctgc tcaatctgtc gctgctgagc
     1021 cgcaagcaac gcggcgagtt gtatcgccat ctcgtaaatg aagcacgcaa ggaagtcgag
     1081 agtccctgaa ggcggggccg ggccgccgga cccgttgaat cgaaagaacc catccccact
     1141 cagtccgctc agaagtgaat aaagtcaaaa tgcacgcaag gatgaacagc agacgggttc
     1201 gcggggccgc cgcgaagacg ctggtcggtt ggctcctggt cgtcgccgcc ggggccgcca
     1261 tttaccacta tacgcgaccg ggcacggccg aggaggccgg cggggggggc ggcccggagg
     1321 gcgcgccgcc gccggaagtc gccgtcgagg tggtcgagcc gcggccggtc acgctcaaga
     1381 cggagctgcc cgggcggacg gcggcgcggc tcatcgcgga aatccggccg caggtgagcg
     1441 gtctgatcca gcagcggctg ttcacggaag ggtcggacgt caagcaaggg gaggtgctgt
     1501 acatcatcga tccggcgccg ttcgaggcgg aagtggcgca ggccgaggcg aacctgacgg
     1561 gcgcgcggca ggcggcggat cgggcgcggg ccgcgattgc ggccgccgag gcggcggtgc
     1621 ggcagcggca ggccgtgctt acgctggcgc gcaccgatct ggaacggatc gagcagctgg
     1681 cgagcgaagg gattgtttcg cagagcgagc gggacaagat ggtgacggag gcgcgcgtcg
     1741 cggaggcggc gctggtgtcg gccgaggcgg aactggccag ccagcgcgag gcggtgggcg
     1801 tcgcggaagc cgcgatcggg caggcgcagg cggcgctgga agcggcgcgg atccaactgg
     1861 accacacgcg gatcgtggcg ccgatctcgg gacggatcgg gatctcgcag gtgacggtcg
     1921 gggcgctggt gacggcgcat caggcgaccc cgctggcgac gatccagcag ctcgatccga
     1981 tctatgtgga tgtgtcgcaa tccacgtccg aactgctggc gctgcgccgg cggcttgaat
     2041 cggcggagtt gacttcagac accgcgacga tcgacacggt cggcctgcgg ctcgaggacg
     2101 gcagcgcgta tgaccacacg gggacgctgg cattccgcga cgtcaccgtg gatcagagca
     2161 cggggtcggt cacgctgcgc gccgtatttc ccaaccccga ggggctgttg ctgcccggca
     2221 tgttcgtcca tgcgatcgtg accgaaggga cgcgcaacga tgcgatcctc gtctcgcagc
     2281 aggcggtctc gcgcgatccg aagggcaatc cgctggccct gacggtgaac gcggagggga
     2341 aggttgaaca gcggatgctg acgctggatc gcgccatcgg cgaccagtgg ctggtgacaa
     2401 gcggcctggc gcgcggcgac cgggtgatca tcgaagggat gcagcgcgtg aggccgggcg
     2461 cggcggtcgt cgtggcgtcg gatgccgggg gagaaaccgc ggcggagacg cgtgtttcgg
     2521 attcgtcagt ttcgtcagtt tattcgggga acggcgcctg atgctttcct ccttctttct
     2581 caagcggccg gtcttcgcct gggtgatcgc aatcgtgctg atgctcgccg gggcgctggc
     2641 gatctaccat ctgcccgttt cacagtatcc gccgatcgcg cccccgtcga tcgcgattga
     2701 cgcgttctat ccgggcgcgt cggcgcagac ggttgaggac agcgtggtgc agatcatcga
     2761 gcagaagatg accggcttcg acaatctgct ctacacgtcg gcgacgagcg atgcctcggg
     2821 cgccgggcgc gtcgagctga cattcgcacc cggcaccgat ccggacctgg cctgggcgca
     2881 ggtgcagaac aagctgcagc tggcgatggc gagtctgccg gaggtggtcc agcgcgcggg
     2941 cgtgaaggtg agcaagtcga cgcgcaatta cctgatcatc gtcgggcttg tttccgagga
     3001 cggcagtatg gatagcgccg acctgaacga ctatgcacag tcaagcatcg agaaggtgct
     3061 ggcgcgcgtg ccgggggtgg gcgaggtcaa cacgttcggg tcgcagtatg cgatgcgggt
     3121 ctggctgaat ccggagaagt tgacgcagta tcagctgacg atcacggacg tggtgcaggc
     3181 gctggcgaag tacaatgtcg aggtgtcggc ggggcagttc ggcggggcgc cggcggtgga
     3241 agggcagcgg ctgaacgcgt cgatcatcgt gcagaatctg ttgcagacgc cggaggaatt
     3301 cgcggcgatt ccgctgcgga cgaattccga tggctcggtc gtccgcgtca gcgacgtggg
     3361 acggaccgaa ctgggaaccg agttctacgg ttcggacgcg ctgttcaacg gcaagccgtc
     3421 ggcggcgctc gcgatacgcc aggcggcggg ggcgaacgcg ctcgatacgg ccgacgccgt
     3481 caagaagaag atggaggagc tttccgcgtt ctttcccccc gggatgaagg tgatctatcc
     3541 gtacgacacg acgccgttcg tgaaggccgc gatggaagag gtggtcaaga cgctgttcga
     3601 ggcgatcgtg ctcgtgttct tcgtgatgta cgtcttcatg ggcaatctgc gggcgacgct
     3661 gattccgacg atcgcggtgc cggtggtgat tctggggacg ttcgcgacgc tcgcgctgtt
     3721 cggtttttca atcaacatgc tgacgatgtt cgcaatggtg ctggcaatcg gcctgctggt
     3781 ggatgacgcg atcgtcgtgg tggagaacgt ggagcggatc atgaccgagg aggggctggc
     3841 gccgcgcgcg gcgacggaga aatcgatgcg cgagatcacg agcgcgctga tcgggatcgg
     3901 gctggtgctc tcggcggttt ttggtccgat ggcgtttttc ggcagctcga cgggggtgat
     3961 ctaccggcag ttctcggtga cgatcatcgc ggcgatggtc ctgtcggtgg tcgtggcgct
     4021 gatcctgacg ccggtgctgt gcgtctcgtt gctcaagccg gtcgcgcggc atcattcgcc
     4081 gggcgaacac gcgattttct tcatgcggcc gtttttccgc tggtttgaac gggtgttcga
     4141 ccgcgcgcgg gtcacctacc tggccgtcgt cgggcgcatc ctggtccgca aaatgcggta
     4201 tatcgtgatt tttctgctga tcgtcggcgg catgttctat ctgttccggc ggatgccgac
     4261 cgcctacctg ccggatgaag accaggggat tctgttcgtg caggcgacgc ttcccaccgg
     4321 atcgacgctg gagcagaccg aggcggtgat ggcggaggtg cgcgaatact tcatggagca
     4381 agagcacgag gcggcgcaat cgacgctgac gatcgcgggg cggagttttt cgggcgtggc
     4441 gcagaacgcc gggctggcgt tcgtgaagct gcgcgactgg gatgaacgcc acgcgccgga
     4501 attgaaggtc aaggcggtgg tcaaccgcgc gatggatcgg ttcgcgagga tccgcaacgc
     4561 gagcgtgttt gccttcccgc cgccggcggt gattgaactg ggccaggccg ggggtttcga
     4621 ctttcaactg ctggaccgcg ggggcgtggg ccacgacgcg ttgatggagg cgcgcaatca
     4681 gatcctgtcg atggcggcgg aggaaccgcg gctgacgcga gtgcgcgcca acgggctgga
     4741 ggacgtgccg cagtatcgcg tcgaggtgga ttggggaaag gccggcgcgc tgggggtggc
     4801 gatcgatgcg atccatgcga cgatctccgc ctcgttcggg agcgcttacg tcaatgactt
     4861 catccaggga gggcgcgtca agcgggtata tgtgcaggcc gacgcgccgt atcggatgct
     4921 gcccgaggac ttgaagaagc tgtatgtgcg caatccggag gggcggatgg tgccgttcgc
     4981 atcgttcgcc acggggcatt ggacgtatgg ctcgcccaag ctggaacggt tcaacggctt
     5041 tccgtcgatc aacatcctgg gtgagccggc gccgggccgg agctcgggcg aagcgatgca
     5101 gttgatggaa gagttcgtca agaagctgcc gcaggggatc gcgcatgact ggacggggtt
     5161 gtcgtatcag gaacggatgg ccgggtcgca ggcgccgatt ctgtatgcgt tttcgattct
     5221 ggtgatcttc ctgtgtctgg cggcgctgta tgagagctgg ccggtgccgg tctcggtgct
     5281 gctggcgttg ccgctggggg cgatcggcgg ggtgatcgcg tcgacgctgc gcgatctgcc
     5341 caacgacgtg tatttccaga tcggcctgct gacgacgctc gggctgacga cgaagaacgc
     5401 gatcctgatc gtgcagtttg ccaaggcgcg gtacgatgag gggatggacc tggtggaggc
     5461 gacgctggaa ggggccaaac tgcgggtgcg gccgatcgtg atgacgtcgc tggcgttcgg
     5521 gttcggcgtg atcccgctgg cgttcgccaa cggggcgggg gcggg
//
LOCUS       DAOPCO010000261         5556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12562_length_5556_cov_5.760044, whole genome shotgun sequence.
ACCESSION   DAOPCO010000261 DAOPCO010000000
VERSION     DAOPCO010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12562_length_5556_cov_5.760044"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1569)
                     /locus_tag="PLS90_14255"
     CDS             complement(340..1569)
                     /locus_tag="PLS90_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK03606.1"
                     /translation="MLRFPFHLITRAGALILPFILTGCLAHRGQPPIAAADLHRLPAG
                     IETRWASPENWLGAKGQGATTRGGRKGSPCFPLKAGESKVLADVQGHSGMVRRIWITI
                     SDRPAQFLRGLKLEMFWDGAATPAVAVPLGDFFCQGLGQMTAFENALFSSPEARSFNC
                     LIPMPFREGMKIVVTNESADDLPLMFYDIDYTLGDRHGDDMLYFHAHWRRENPTTLRQ
                     DFEILPRVSGRGRFLGTLVSVIVNQEDYLDSWWGEGEVKVYLDGDRELPTLVGTGTED
                     YIGTGWGQGQYANHFQGCPVADHERMRYAFYRLHIPDPIYFQQDCRVTLQQIGYVGEG
                     TGKALKELGKTIHAEYEQGMAPVPPDDVGTRVFLVERQDDVASCAYFYLDRPTNDLPP
                     LAPFADRIAALTDEPED"
     gene            complement(1713..2876)
                     /locus_tag="PLS90_14260"
     CDS             complement(1713..2876)
                     /locus_tag="PLS90_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPK03607.1"
                     /translation="MDMIAQMWELTGFYFLNWQQVLMIGIGMVLVWLAVAKGFEPLLL
                     VPIGFGVIVGNMPFDPTDAISVYATGSEGHPEGTFALIYFLVSHEVLPPLIFMGIGVL
                     TDFSYMISNPRLILLGAAAQLGVFVTFLGAVLIGDKIEGIYITEAAAAAIGIIGGADG
                     PTSIYIANRMAPGWLGAIAIAAYSYMSLVPVIQPPVMRLLTTRKERMIRMKPPREVSK
                     QLRVMFPVIGFLITCLFIPSAMVLLGMLFLGNLIRESGVTDRLARTIGGPFIDSVTVL
                     LGFSVGLATTWEKFLNTEVLVIFALGAFAFTIATAGGILFAKLMNKFSKDPINPLVGS
                     AGVSAVPMAARVSQQVAFKDDPNNYILMHAMAPNVAGVIGTAVAAGVFIAMLS"
     gene            complement(2946..3332)
                     /locus_tag="PLS90_14265"
     CDS             complement(2946..3332)
                     /locus_tag="PLS90_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPK03608.1"
                     /translation="MRMRVSVNGVAYDVEVEVLDEEADLSSAARPAAPSANAPRPPVA
                     PVAAPAPAPAAGGEASTLASPIPGTIVEILVKPGQPVAANETVIIIDAMKMNTPVAAP
                     AARTVKEVLVKAGDAVKMGQPLLTFE"
     gene            complement(3338..3856)
                     /locus_tag="PLS90_14270"
     CDS             complement(3338..3856)
                     /locus_tag="PLS90_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HPK03609.1"
                     /translation="MKRRWNLATWRPCVPLALAAALCVFGLVDVAAQPDSVGAAASPA
                     APTVGYIDWGRVWEITVGGMVVVMLVLSAMATAVALLNRIEIRLHARRDAEAAARPPA
                     GEPAGVTPAAPAPEAASAGNEVDVVIAAAVAAFLQGRRFRVSRVRLAEPASSWAQMGR
                     VGIQTSHNIQKR"
     gene            complement(3881..5494)
                     /locus_tag="PLS90_14275"
     CDS             complement(3881..5494)
                     /locus_tag="PLS90_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014303088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPK03610.1"
                     /translation="MSPDTLVSDKKQAEARQPAKPLETTAAKLKDLTARRKQISLGGG
                     EAALKKRKESGRLNARERLDLLLDPSSFQEMYQHRKHRCTNFGMERKDLAADGVVTGL
                     GAIDGRPVCIASQDFTVAGGSLGEAHGQKIVEMMKMSLKCGSPFIMINDSGGARIQEA
                     VDGLNSYGEIFYHNVLASGVVPQISLICGPCAGGAAYSPALTDFIIMVRGNKMFITGP
                     EVVKSVTGEEISAEQLGGADTQATVTGNIHFIADSDEQAFAICRKLLSFLPSNNLQDP
                     PSRNTGEVDLTEIPALDKLMPDSAREAYDVREVIRLVIDGGDFLEVMEHWATNIVIGF
                     ARIDGNVVGIIANQPLVMAGVLDINASDKASRFIRFCNAFNIPIVSFVDVPGYMPGVN
                     QEHGGIIRHGAKLLFAYSATTTPKITVILRKAYGGAYLAMCSKSLGADRTGAWPTGEI
                     AVMGAEGAVPIIFRREMEQAEDKEARRQELLDEYRVKFGNPYLAASRGLIDDVIQPRE
                     TRRYIAMSLRALQSKRDLRPPKKHGLMPL"
BASE COUNT          927 a         1605 c         2078 g          946 t
ORIGIN      
        1 gccgggagcc gaattttggg gttggctttt tggggttttg tggagtgcgg cggcaaagcg
       61 ggttggctat cgctccgggt gaatcagaac atcgccgcaa acaccgggtt tcgagagccg
      121 aggacgaggg acgagagccg ggagccgaat tttggggttg gctttttggg gttttgtgga
      181 gtgcggcggc agagaccgcg cagcggtcgt cgacgccgct ttggatcggt cggaatcagc
      241 tgaggtgatc ggatccaaag cggtgtcgac gcgcgtcggg cgccgcgctt tgccaccgca
      301 ctccaagaag cgggaagacc tcgccgggga ggtgaggcgt caatcctcgg gttcgtcggt
      361 cagcgccgcg atgcgatcgg cgaatggggc gagggggggc aggtcgttcg tcggccggtc
      421 caggtagaaa taggcgcaac tggcgacgtc gtcctggcgc tcgacgagga acacgcgcgt
      481 gccgacgtcg tcgggcggaa cgggcgccat cccctgctca tactccgcat ggatcgtctt
      541 gcccaattcc ttgagggctt tgccggtccc ttcgccgaca tagccgatct gctggagcgt
      601 cacgcggcaa tcctgctgaa agtagatcgg gtcggggatg tgcagccggt agaaggcgta
      661 gcgcatgcgt tcatggtcgg cgaccgggca gccctggaag tggttggcat attgcccctg
      721 cccccagccg gtgccgatgt aatcctcggt gccggtgccg acgagggtgg gcagctcgcg
      781 gtcgccgtcg aggtagactt tgacttcgcc ctcgccccac caggaatcga ggtaatcctc
      841 ctggttgacg atgacgctga cgagggtgcc gagaaagcgt ccgcggccgg agacgcgggg
      901 gaggatttcg aagtcctggc gcaacgtggt ggggttttcg cggcgccaat gggcgtggaa
      961 atagagcatg tcgtcgccgt ggcggtcgcc cagggtgtaa tcgatgtcgt agaacatgag
     1021 ggggagatcg tcggccgatt cattggtgac gacgatcttc attccctcgc ggaagggcat
     1081 cgggatgagg cagttgaagc tgcgcgcctc ggggctggag aagagggcgt tttcgaaggc
     1141 ggtcatctgg ccgagcccct ggcagaagaa gtcgccgagg gggacggcga cagcgggcgt
     1201 ggcggcgccg tcccagaaca tttcgagctt gagcccgcgc aggaactggg cggggcggtc
     1261 gctgatcgtg atccagatgc ggcggaccat gccgctgtgg ccctggacgt cggcgaggac
     1321 cttggattcg ccggccttga gggggaaaca gggggagccc ttgcggccgc cgcgcgtcgt
     1381 ggcgccctgg cctttcgcgc cgagccagtt ttcggggctg gcccagcgcg tttcgatgcc
     1441 ggcggggagg cggtgaaggt ccgccgccgc gatggggggc tgcccgcggt gggcgaggca
     1501 gccggtcagg atgaacggca ggatcagcgc gccggcgcgc gtgatgaggt gaaaggggaa
     1561 acgcaacaca gcaaacctcc gcaacgggat tgagggggca acggtccggc gcggagggat
     1621 tcgggcaggg gcatgccgac agcgtcgcgc ccaacagacc ccctggaatt tatgcagtcg
     1681 gcggttggaa gatcctcccg agaatcccgg cgtcagctga gcatcgcaat gaagacgccg
     1741 gcggcgacgg cggtgccgat gacgccggcg acgttgggcg ccatggcgtg catcaggatg
     1801 taattgttgg ggtcgtcctt gaacgcgacc tgctggctga cgcgggcggc catggggaca
     1861 gccgagacgc cggcgctgcc gaccaggggg ttgatcgggt ccttgctgaa cttgttcatc
     1921 agcttggcga agaggatgcc gccggccgtc gcgatggtga aagcgaacgc gcccagggcg
     1981 aagatcacaa ggacctcggt attgaggaac ttctcccaag tggtggccag cccgaccgaa
     2041 aagccaagca ggacggtgac ggaatcgatg aacgggccgc cgatcgtccg ggcgaggcga
     2101 tcagtcacgc ccgactcgcg aatcagattg cccaagaaga gcatgccgag caggaccatc
     2161 gccgagggaa tgaaaaggca ggtaatcagg aagccgatga ccgggaacat gacgcgcagc
     2221 tgtttggaga cctcgcgcgg gggcttcatg cggatcatgc gttccttgcg ggtcgtcaac
     2281 aggcgcatga cggggggctg gatgaccggg acgagcgaca tgtagctgta ggcggcgatg
     2341 gcgatcgcgc cgagccagcc gggcgccatg cggttggcga tataaatcga agtggggccg
     2401 tcggcgccgc cgatgatgcc gatggcggcg gcggcggcct cggtgatgta gatcccctcg
     2461 atcttgtcgc cgatgaggac cgcgccgagg aacgtgacga agacgcccaa ctgggcggcg
     2521 gcgccgagca ggatcagcct ggggttggag atcatgtaac tgaaatcggt cagcacgccg
     2581 atgcccatga aaatcagcgg gggcaagact tcatgggaga cgaggaagta gatcagggcg
     2641 aaggttccct cgggatggcc ctcggagccg gtggcataga cgctgatcgc atcggtcggg
     2701 tcgaacggca tgttgccgac gatgacgccg aagccgatcg ggacgagcag gagcggctcg
     2761 aagcccttgg cgacggcgag ccagaccagc accatgccga tgccgatcat cagcacctgc
     2821 tgccaattga gaaaatagaa gccggtcagt tcccacatct gggcgatcat gtccatcgga
     2881 tcagtactcg cttccgccgt cggagatggt cgttgcaccg gcgcggccgg gatggccgcc
     2941 gggcgttatt cgaaggtcag gagaggctga cccattttga cagcgtcgcc cgctttgaca
     3001 aggacctcct tgacggtgcg cgccgcgggc gccgccaccg gggtgttcat cttcatcgcg
     3061 tcgatgatga tcaccgtttc attggcggca actggctgac ccggcttgac caggatctcg
     3121 acgatggtac cggggatcgg gctggcgagg gtgctggcct cgccgccggc ggccggcgcc
     3181 ggggcggggg cggcgacggg cgccaccggc gggcgcgggg cattcgcgct gggagcggcc
     3241 ggccgcgccg ccgaggacaa atcggcttct tcgtccagca cttcaacctc gacatcgtaa
     3301 gcaacgccat tgacgctgac tcgcattctc attgcggtta cctcttctgg atattatgtg
     3361 aagtctggat gccgacgcgt cccatctggg cccacgagga ggcgggttcg gccagccgga
     3421 cgcgactgac ccggaagcgc cggccctgga ggaacgccgc gaccgccgcc gcgatgacca
     3481 cgtccacttc gttgccggcc gaggcggctt cgggcgcggg ggcggcgggg gtgacgccag
     3541 ccggttcgcc ggcggggggc cgcgccgccg cctcggcatc gcggcgggca tgcagcctga
     3601 tttcgatccg gttgagcagg gccacggcgg tcgccatcgc cgacaggacg agcatgacaa
     3661 cgaccatgcc gccgaccgtg atctcccaga cgcgacccca atcgatatag ccgaccgtgg
     3721 gggcggcggg cgacgccgcc gcgccgacgg agtcgggctg tgccgccacg tcgaccaggc
     3781 cgaaaacgca gagggcggcg gccagggcca gcgggacgca aggtcgccag gttgcgaggt
     3841 tccaccgtcg cttcatcgtc gcgggctccc gtgaatcgga ttacagcggc atcaagccgt
     3901 gttttttcgg cggccgcaga tcgcgtttgc tctgcagggc gcgcagcgac atcgcgatgt
     3961 agcggcgggt ttcgcgcggc tggatgacgt cgtcgatcag gccgcgcgag gcggccaggt
     4021 aggggttgcc gaacttgacg cgatactcgt cgagcagttc ctggcggcgg gcttccttgt
     4081 cctcggcctg ttccatctcg cggcggaaga tgatcgggac ggcgccctcg gcgcccatca
     4141 cggcgatctc gccggtcggc caggcgccgg tgcggtcggc gccgagcgat ttggagcaca
     4201 tcgccaggta ggcgccgccg taggccttgc gcaggatcac cgtgatcttg ggggtcgtcg
     4261 tggccgagta ggcgaacagc agtttggcgc cgtgacggat gatgccgccg tgctcctggt
     4321 tgacgccggg catgtagccg ggcacgtcga cgaacgagac gatcgggatg ttgaaggcgt
     4381 tgcagaagcg gatgaagcgg gaggccttgt ccgaagcgtt gatgtcgagg acgccggcca
     4441 tgacgagcgg ctggttggcg atgatgccga cgacgttgcc gtcgatgcgg gcgaagccga
     4501 tgacgatgtt ggtcgcccag tgctccatga cctcgaggaa atcgccgccg tcgatgacca
     4561 ggcggatgac ttcgcgcacg tcgtaggctt cgcgggcgct gtcgggcatg agcttgtcga
     4621 gggccgggat ctcggtcaga tcgacctcgc cggtgttgcg gctggggggg tcctgcagat
     4681 tgttgctggg gaggaacgag aggagcttgc ggcagatggc gaaggcctgc tcgtcgctat
     4741 cggcgatgaa gtggatgttg ccggtgacgg tggcctgggt gtcggcgccg ccgagctgct
     4801 cggccgagat ttcctcgccg gtgaccgact tgaccacctc cgggccggtg atgaacatct
     4861 tgttgccgcg caccatgatg atgaaatcgg tcagcgcggg ggaataggcg gcgccgccgg
     4921 cgcaggggcc gcagatcagg ctgatctgcg ggacgacgcc cgaggcgagg acgttgtggt
     4981 agaagatttc gccataggag ttgaggccgt cgaccgcctc ctgaatgcgc gcgccgcccg
     5041 agtcgttgat catgatgaac ggggagccgc acttgagcga catcttcatc atttcgacga
     5101 ttttctggcc gtgggcctcg cccagcgagc cgccggcgac ggtgaagtcc tggctggcga
     5161 tgcagacggg ccggccgtcg atggcgccca ggccggtgac gacgccgtcg gcggccaggt
     5221 ccttgcgctc catgccgaag ttggtgcagc ggtgcttgcg gtgctgatac atctcctgga
     5281 acgaactggg gtcgagcagc agatcgagcc gttcgcgggc attgaggcgg cccgattcct
     5341 tgcgcttctt cagcgcggcc tcgccgccgc cgaggctgat ctgcttgcgg cgggcggtca
     5401 ggtccttcag tttggccgcc gtagtttcga gcggtttggc cggttgcctg gcttcggcct
     5461 gtttcttgtc gctgaccaac gtgtcgggtg acatggggaa cttccttctg aatcgtgcgg
     5521 ggaaagggct ggacgggtcg cgggggcgtg gcccgc
//
LOCUS       DAOPCO010000262         5554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12572_length_5554_cov_4.540826, whole genome shotgun sequence.
ACCESSION   DAOPCO010000262 DAOPCO010000000
VERSION     DAOPCO010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12572_length_5554_cov_4.540826"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLS90_14280"
     CDS             <1..715
                     /locus_tag="PLS90_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK03611.1"
                     /translation="YLDKATVGDVAGFVLEPIQGWGGSVIPPDDFFPKLRALCDRLGI
                     LLMADEVLTSWGRTGKWLCMEHWGVLPDVVTLGKGFGNGFPVTCVAVREPYKESFESI
                     SASSSYGGNPMACAAALASTEVIAEENLLEWSSHLGDLALARMLKMKQEHKIIGDVRA
                     RGCLMGIELVKDRQTKEPDNQAGALVYQKAFAKGLAWIPAGHILRMSPPIVMDEATLA
                     RGLDIIDEAIGETEKELGY"
     gene            841..1983
                     /locus_tag="PLS90_14285"
     CDS             841..1983
                     /locus_tag="PLS90_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03612.1"
                     /translation="MQVIRFGIIGCGLMGREFASAAARWCHLTDIAARPEIVAVCNRT
                     AAKMDWFTAHFPTIRQATTDYRELLANDEVDAVYCAVPHNLHEETYCAVIESGKHLFG
                     EKPFGIDRAAYDAIAACLRRHPRVFTRCCSQFPFFPGVQRIGSMIEQGAFGRIIEVEA
                     GFLHSSDLNPAKPINWKRQIKTNGEYGVMGDLGMHICHVPLRAGWRPRNVRAILSNIV
                     PVRPDGKGGMAPCETWDNATLLCEAEDAAGERFPLTLKTQRIAPGETNTWFLRILGTQ
                     AAAEFTTRRPTLLRLLEYQNGRQDWRELDLGYETAFPTITGGIFEFGFGDAILQMWAS
                     YVDELHRGAPRGRFSGTATVEEVGLSHQLFTAALRSQREQATVPVE"
     gene            2581..5370
                     /locus_tag="PLS90_14290"
     CDS             2581..5370
                     /locus_tag="PLS90_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03613.1"
                     /translation="MLLIPLTPRPSTTALILFLLAVRWLPAETPAVVPHPADWPTFRQ
                     NPHLTGRSPLRGDLETTPRVVWRHDLGAQTVPGETFTFADFDGDREHETLVAGTHSLA
                     LRDADGTTLWELRDIPQPTIVQFADFAGNGSQGIIYWTSDGVTATYAIIDCRTGVRTE
                     LFTLQNSFGFRMRYGSILPGVKGMQVCVFWTGDPVDDTWSGHAYLWSFAEGCDRPQLE
                     FEAHPNGLPISPLIAFDDYNGDGVPDLYAIAHEHATVWDMTTGEMLLHGPFSDNIIRT
                     YSGILAIEHFPDRDHPSLLIVNRNLPGAYVVDLVGDKPVRRWKKVVGPLENQYQTAVE
                     IEGGAPDPFRDIDGDGVPEFLLRVKNEHDDGQTRLVVLRAEDGRRLYDGPPLEIISVD
                     ELDGKSPPEILIQADSRLELAHWNGEGFATVWTAEGAQPVFEPATPLQDPTRYTFIRA
                     QFSNPVVWKDSAADHTFFLRTADGIRRARLDGAGAVTLLDAPAADHPVTGFIPEAERM
                     TTVVRGAEGVTEIVRAGEVIGQIPSTTLRRYLPPPPLVGELGGATRIVARTNDARVIS
                     LTPAGESGPLLAQACPTLGDPFADKAFFGLLADIDADGANEFLCSTEHDGAAVIAVDA
                     DGREQLRLRHREEAGNVILIATGRKSGSTGNWIATRWQTREKSWGTLWYATAFDAISG
                     EALWERSNYGKYGEEPLAIGIHVPTAVVDYNGDGGDDLIVACANNFGIIDGTTGADLY
                     GPKNMLDNRPDGIWPAYSKPILADLDGVGTPELIHAMSFASTYVATWESDPLWSIKLT
                     RDRTARRYPGIADLDGDGTRELITSQQNGLLIACNHQVEDGAKRERWQRKLAGPVNDI
                     TAIDLDDDGRDELLVGSADGRLHALKEIDGEPRTLWTVSLEGAVGSPVVADLDGKGRP
                     SILVPTDQGYLYRIE"
BASE COUNT          962 a         1902 c         1779 g          911 t
ORIGIN      
        1 atacctcgac aaggcgacag tgggcgacgt cgccgggttc gtgctcgagc cgattcaggg
       61 ctggggcggc tcggtcatcc cgcccgacga cttcttcccc aagctgcgtg ccctgtgcga
      121 ccgcctcggc attctgctga tggccgacga agtcctgacc agttggggcc gcaccggcaa
      181 gtggctctgc atggaacact ggggcgtcct ccccgacgtc gtgacgctgg gcaagggctt
      241 cggcaacggt ttccccgtca cctgcgtcgc cgtgcgcgag ccctacaagg aaagcttcga
      301 atcgatttcg gccagttcca gctacggcgg caatccgatg gcgtgcgcgg ccgccctcgc
      361 ctcgaccgaa gtcatcgcgg aggagaacct gctggaatgg tcgagccacc tgggcgacct
      421 ggccctcgcg cgcatgctca agatgaaaca ggaacacaag atcatcggcg acgtgcgcgc
      481 caggggctgc ctgatgggga tcgagctggt caaggaccgg caaacgaagg agccggacaa
      541 tcaagccggc gcgctcgtct atcagaaggc cttcgccaaa gggctggcgt ggatcccggc
      601 cggccacatc ctgcggatga gtccgccgat cgtgatggat gaggcgacgc tggcccgcgg
      661 cctggacatc atcgacgagg cgatcggcga gaccgagaag gaactgggct actgagtttc
      721 ggcgcacacc tgatcaaccg gcgggccgga gccgttcccg ctctcctcca catgcccgta
      781 gcattccggc ccgccggccc acctcctcag ggtgcagcac tgcaataaag gaaaccaacg
      841 atgcaggtca tccgctttgg gatcatcggc tgcggcctga tgggccgcga gttcgccagc
      901 gcggcggcgc gctggtgtca tctgaccgac atcgccgcgc ggcccgagat cgtggccgtg
      961 tgcaaccgca cagccgccaa gatggactgg tttacggccc atttccccac gatccgccag
     1021 gccaccaccg attaccgcga gctgctcgcc aacgacgaag tcgacgcggt ttactgcgcc
     1081 gtgccgcaca atctgcacga agaaacctac tgcgcggtga tcgagtcggg caagcacctc
     1141 ttcggcgaga aaccgttcgg catcgatcgg gcggcctacg acgccatcgc cgcgtgcctg
     1201 cggcgccacc cgcgcgtctt cacccgttgc tgctcgcagt ttcccttttt ccccggcgtc
     1261 cagcggatcg ggtcgatgat tgagcagggg gcgttcgggc ggatcatcga agtggaagcg
     1321 gggttcctcc actcgagcga cctgaatccg gccaagccga tcaactggaa gcgccagatc
     1381 aagaccaacg gcgaatacgg cgtgatgggc gatctgggca tgcacatctg ccacgtgccg
     1441 ctgcgcgcgg gctggcggcc gcgcaatgtc cgcgcgatcc tgagcaacat cgtcccggta
     1501 cggcccgacg gcaagggcgg catggcgccg tgcgaaacgt gggacaacgc cacgctgctg
     1561 tgcgaggccg aggacgcggc cggcgaacgc ttcccgctca cgctcaagac gcagcgcatc
     1621 gcccccggcg agacgaacac ctggttcctg cgcatcctgg ggacgcaggc ggcggccgag
     1681 ttcacgaccc gccggccgac gctcctgcgc ctgctggagt atcagaatgg ccggcaggac
     1741 tggcgcgagc tcgatctcgg ctacgagacg gccttcccga cgatcaccgg cgggatcttt
     1801 gaattcggct tcggcgacgc gatcctgcag atgtgggcgt cctacgtgga cgagttgcat
     1861 cgcggcgcgc cgcgcggccg cttcagcgga acggcgaccg tcgaggaagt cggcctcagc
     1921 catcagctgt ttaccgccgc cctccgctcc cagcgcgaac aggccaccgt gcccgtggag
     1981 taaggccgaa ggggtgagac gcgcctttgg ggcgaacgct cctgagcgct ggcggctcct
     2041 gtttgccgat cgctgcagtc cgcgaagcgg gcgaccggta tcaagcgcag ggtgatggcc
     2101 gcaggccgga accctgcgtc gcccccccaa tgattcaagc ccctgccgga tccttcagcc
     2161 ggtcagtccc ctcagggatg gcggacgttc agcccgctac ttcagtggcg gcaacccgga
     2221 tatccgtggc gacagagtcc caaagggatg acagaaccgc tcgagtcgat cacgaaggcg
     2281 agggacatgg gacccgtttt cggttgagct tttcacattg cctctcgcgt cacctccagg
     2341 atgaaatgcc ccgcatgcgt cttgtgtgtc cgttatgtcc cttgtgtccc ttatgtccct
     2401 tgtgtccctt atgtctctca tgtccctcag gccccttctg caacacggat tcttcggcgc
     2461 gcgggatcgc gacatggggg ccggcggaaa aaagccttgc ctgttcctga cactcgggta
     2521 tcatgcagct gcatcccccg gcttcttccg tcccccccgg gcccgtcaca ggaggcgact
     2581 gtgcttctca ttccgctcac ccctcggcca tcgaccacgg ccctgatcct cttcctgctg
     2641 gccgtgcgct ggctccccgc cgaaactccg gccgtagtcc cgcaccccgc cgactggccg
     2701 acgttccgcc agaaccccca cctgaccggt cgctcgccgc tgcgcggcga cctggaaacg
     2761 acgccgcgcg tcgtctggcg tcacgatctg ggagcgcaaa ccgtcccggg tgaaacgttc
     2821 accttcgccg atttcgacgg cgaccgcgaa catgagacgc ttgtcgcggg gacccattcg
     2881 ctggcgctgc gcgatgccga cggcacaacc ctttgggaac tgcgcgacat cccccagccg
     2941 accatcgtcc aatttgccga ctttgccggc aacggcagcc aggggatcat ctactggacg
     3001 tcggacggcg tcacggcgac ctatgcgatc atcgactgcc gcaccggcgt ccgcaccgag
     3061 ctgttcacgt tgcagaatag cttcgggttc cgcatgcgct acggttcaat cctccccggc
     3121 gtgaagggca tgcaggtctg cgtcttctgg accggcgacc ccgtggacga cacctggtcc
     3181 ggtcacgcgt atctgtggtc gttcgccgag ggctgcgacc ggccgcagct cgaattcgaa
     3241 gcccatccga acggtctgcc gatcagcccc ctgatcgcgt ttgacgacta caatggcgac
     3301 ggcgtcccgg acctgtatgc gatcgcgcat gagcatgcga ccgtctggga catgacgacg
     3361 ggcgaaatgc tcctgcacgg cccgttcagc gacaacatca tccgcaccta ctcgggcatc
     3421 ctggcgatcg agcatttccc ggatcgcgac catccgtcgc tgctgatcgt gaaccgcaac
     3481 ctgccgggcg cctacgtcgt cgacctggtt ggcgacaagc cggtccggcg ctggaaaaaa
     3541 gtcgtcggcc cgttggaaaa ccagtatcag accgcggtgg aaatcgaggg gggcgcgccc
     3601 gatccgttcc gcgacatcga tggcgacggc gtccccgaat tcctcctgcg cgtcaagaac
     3661 gagcacgacg acgggcagac gcgcctggtc gtcctgcgcg ccgaggacgg ccgccggctg
     3721 tatgatggcc cgccgctgga gatcatttca gtcgatgagc tggacgggaa atcgccgccg
     3781 gaaatcctga tccaggcgga cagccggctg gaactggctc actggaacgg cgaaggcttc
     3841 gcgacagtct ggacggccga aggcgcgcag ccggtcttcg agccggccac cccgctgcag
     3901 gacccgaccc gctacacctt cattcgcgcc cagttcagca accccgtcgt ctggaaggac
     3961 tccgccgccg atcacacgtt cttcctgcgc acggccgatg gcatccggcg cgcccggctg
     4021 gacggcgccg gcgccgttac attgctcgac gcgcccgccg ccgatcatcc cgtgaccggg
     4081 ttcatccccg aggccgaacg gatgacgacc gtcgtgcggg gggccgaagg cgtcacggag
     4141 atcgtccgcg ccggcgaggt gatcggccaa atcccgagca caaccctgcg gcgttatctg
     4201 cctcccccgc cgctggtggg cgagttgggc ggcgcgacgc gcatcgtggc gcgcacgaac
     4261 gatgcccgcg tgatcagcct cacgcccgcc ggcgaatccg gcccgctgct ggcccaggcc
     4321 tgccccaccc tcggcgaccc gttcgccgac aaggccttct tcggcctgct ggccgacatc
     4381 gatgccgacg gcgcgaatga attcctgtgc agcaccgagc acgacggcgc ggccgtcatc
     4441 gcggtcgatg ccgacggccg ggaacaactg cgcctgcgcc accgcgagga ggcgggcaac
     4501 gtgatcctca tcgccaccgg tcgcaagagc ggctcgaccg gcaactggat cgccacccgc
     4561 tggcagacca gggaaaagtc ctggggcacg ttgtggtatg caaccgcgtt cgacgcgatc
     4621 agcggcgaag cactgtggga acgctccaac tacggcaagt acggcgaaga gccgctggcg
     4681 atcgggatcc acgtcccgac ggcggtcgtc gattacaacg gcgacggcgg cgacgacctt
     4741 atcgtcgcct gcgcgaacaa cttcgggatc atcgacggca cgaccggcgc cgatctgtat
     4801 ggccccaaga acatgctgga caatcgcccc gacgggatct ggccggctta ttcgaagccg
     4861 atcctggccg atctggacgg cgtcggcacg cccgaattga ttcatgccat gtcgtttgcc
     4921 tcgacctacg tcgccacctg ggaatccgat ccgctgtggt cgatcaaact gacgcgcgat
     4981 cgcaccgcgc gccgctaccc cggcatcgcg gacctcgatg gcgacggcac gcgcgagctc
     5041 atcacgagtc agcagaatgg gctgctgatc gcctgcaatc atcaggtgga ggacggagcg
     5101 aagcgggaac gctggcaacg gaagctggcg gggccggtca acgatatcac ggcgatcgat
     5161 ctggacgacg acgggcgcga tgaactgctg gtcggctcgg ccgacgggcg gctccacgcc
     5221 ctgaaggaaa tcgacggcga gccgcggacg ctctggacgg taagcctgga gggcgccgtc
     5281 ggctcgccgg tcgtcgccga tctggacggc aagggccgcc cgtccatcct cgtcccgacc
     5341 gatcaaggct atctttaccg gatcgagtga acggccggga gtctggttga atgaatctcc
     5401 cgccgagaat tcggcccttg aaacgggaaa gacttgggat gttttcccga agcatgcggg
     5461 gcgcttcagt tgagggccgt gctcaaccgg ttcggacggc cgtggccttg cggatccgct
     5521 cattgccagt cccgcccggg gcggcagact tcag
//
LOCUS       DAOPCO010000263         5538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12610_length_5538_cov_4.722962, whole genome shotgun sequence.
ACCESSION   DAOPCO010000263 DAOPCO010000000
VERSION     DAOPCO010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_5538_cov_4.722962"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1107
                     /locus_tag="PLS90_14295"
     CDS             430..1107
                     /locus_tag="PLS90_14295"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK03614.1"
                     /translation="MRQMPGKPRSRGYSLVEVMIAMVILSVMTSVIITSFIQASRSSR
                     INSNAVAAKNIAQGYFEMLAIEDFERVGNTEHPDYIVPADYNNEYEDKELTDADPVWL
                     DQALGIRCAVDFEFRGFGIAENGSSSMLVDNDANWEPDEWKGHTLFIVSGVGEGQFVE
                     IAGNGQTTLDLASSLAFPPAAGDRYMINNGKTVRITTTWTYMGRQYQQSIESLICNFE
                     GSDDFGF"
     gene            1091..1735
                     /locus_tag="PLS90_14300"
     CDS             1091..1735
                     /locus_tag="PLS90_14300"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03615.1"
                     /translation="MTLASEILSRLLWRRFPVPRRRRQGFTLTEVMIAAAVSMFVAVG
                     AFSLTIMSGRLDKSIASQQRFMKDARRAIEWLNRELRVATTDVMSPISVYDDDGDAAV
                     RGNRVEFSRSGEAAGLRAFYLESDDDDMMTPWDNRLFYDPNTGAAGDEILVSRDIAPV
                     NPNGAFQYEGASEPVVIQMRTGDPANSTNEQEDWANSGANLQGVEINIRIAPRN"
     gene            1792..3516
                     /locus_tag="PLS90_14305"
     CDS             1792..3516
                     /locus_tag="PLS90_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03616.1"
                     /translation="MQRKINRLLRSSGPRRGSALVLVLISFMLLMMMSGGVLLVSTQQ
                     TRDRVRYEAYKDEFAAAELAINKAFGHIVFMVRNGTPDLLDEIDNINAPVLDGYDFPA
                     FEVAETFNGNETVPEGEKWAGLNLHRERYSIDVTAVKTGGSADRFERPGVSMTQNLEI
                     TYIPLYLFAIFYDPLMEIAPGPPMVVNGLVHANGDAYIQANEKLDFQKNVSVAGRFYH
                     GRAPGSGQSDSSGTVRFTKPPDTLVSMLQGGSWLDSRLSNWASLATATWGGGLRDKSH
                     GAPQLNLPIPDGVNPYALIERESVSDGFALQNEKFERKAGLKILADSAGNVTAVDKYG
                     NPVNLWYPDPGNPTLKKWIYSKTTFYDAREGKTVTSIDLNLGNMVEGGIMPSNGILYV
                     SNEDQSGKPGVVRLTNGAKLPQSGVVEGFTVATDDPLYIKGDYNTVNKTLAMVACDSL
                     MILSNAWNDANSTNYSNRIASETTVNTVCMQGNVPTGGGYYSGGVENYFRFLENWSGK
                     DLNFSGSIILMWASQKFLGKWGQSNVYSPPNRKWSWDTALGGINGPPGAPRVVELEKK
                     DWKMASAD"
     gene            complement(3592..4197)
                     /gene="gmk"
                     /locus_tag="PLS90_14310"
     CDS             complement(3592..4197)
                     /gene="gmk"
                     /locus_tag="PLS90_14310"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPK03617.1"
                     /translation="MSLYRFQRRGLLFVISAPSGGGKSAVLTRLLASEADLHYSVSVT
                     SRAPRPDEVEGRDYHFVSRAEFESLVQQDVFYEWAEVHGNLYGTRSDTVEGALAEGRD
                     VALDLDVQGGLSAKYRSPDAVLVFLMPPSFQVLEQRLRQRATDSEEAIRMRLENARRE
                     MIYWRQYNYLVVNEDIDESVEKVRRILYAERHRVSRLKLLQ"
     gene            complement(4202..4582)
                     /locus_tag="PLS90_14315"
     CDS             complement(4202..4582)
                     /locus_tag="PLS90_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK03618.1"
                     /translation="MRGADDRLRTRMLSRPEVRREYDKLEEEFALFDEFLKARRESGM
                     SQADVARKIGTTQSAIARLESPAGKHSPSLATLRRYAEAMGYRVEIRLVKKEEAGQRG
                     SQAKSGKRARSRRRVSARQPIVKD"
     gene            complement(4566..4712)
                     /locus_tag="PLS90_14320"
     CDS             complement(4566..4712)
                     /locus_tag="PLS90_14320"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPK03619.1"
                     /translation="MVRIFHCTLIGRRIVFLHQFLKKSGKTPRRHLEIARSRMTEVKH
                     ARRR"
     gene            complement(4944..>5538)
                     /locus_tag="PLS90_14325"
     CDS             complement(4944..>5538)
                     /locus_tag="PLS90_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPK03620.1"
                     /translation="LPSVTLVHKGNIMKYTEGAFREWGYEEAKENFREQIVTEDELYS
                     EYDGKMPAGKVLIKDRIADSIFQQILLRPDEYSVLATMNLNGDYISDAAAAQVGGLGM
                     APGANIGDNAAIFEATHGTAPKYAGKNMVNPGSLILSAVMMLEHLGWNEAAELVIRGI
                     EGAIAAKTVTYDLERQISGATKVSTSGFAEAIVKQMG"
BASE COUNT         1011 a         1967 c         1592 g          968 t
ORIGIN      
        1 ggcgcgcttg tcggcgcggg cgtcggccgc gcctgttccc gccgcgccgc accaccccct
       61 tccgatctcc agttccatac gatcaggatc agccccgcga gcgccacgac ggccagcagt
      121 ttcttcatcg cgcctctccc cccgggttgg cccggagatc ctccgccttc tgtctccttt
      181 ttatagcact tggcgccggg tgaaagcttt cccttcggca ctgccgatga tttccgagga
      241 gccgcgcctg ctccgccgcg gacgccggct cttctcccct ccggttgcgc cggcgggagg
      301 gaaacgggcg tccgcgattg cggacactcg tcatccgcgt gcgacaaccg cccgatagac
      361 ccctcagaga acatgcagag cggcggcggg cgatgccgaa tccaccgcgt tggccggaaa
      421 gagaggccca tgagacagat gccaggcaag ccgcggtcac gaggctactc gctggtggag
      481 gttatgatcg ccatggtgat cctgtcggtc atgacctcgg tgatcatcac gtcgttcatc
      541 caggcctcgc gatcctcgcg catcaacagc aacgccgttg ccgccaagaa catcgcgcag
      601 ggttatttcg aaatgctcgc gatcgaggac ttcgagcgcg tcggcaacac ggaacatccc
      661 gactacatcg tgccggccga ttacaacaat gaatatgaag acaaggaact gaccgacgcc
      721 gacccggtct ggctcgacca ggcgctgggg atccgctgcg cggtggattt tgagttccgc
      781 ggctttggca tcgcggaaaa cggcagctcc tcgatgctgg tcgacaacga cgccaactgg
      841 gagcccgacg agtggaaggg ccacacgctg ttcatcgtca gcggcgtcgg cgagggccag
      901 ttcgtcgaga tcgccggcaa cggccagacc acgctcgacc tggcctcgtc gctggctttc
      961 cccccggcgg ccggcgaccg ctacatgatc aacaacggca agactgtccg catcaccacc
     1021 acctggacct acatggggcg ccagtatcag cagtcgatcg agtcgctgat ctgcaacttc
     1081 gaggggagtg atgactttgg cttctgagat cctttccagg ctgctctggc ggcggttccc
     1141 tgtcccgcgg cggcgccgcc agggcttcac cctgaccgaa gtcatgatcg ccgccgccgt
     1201 cagcatgttc gtcgccgtcg gcgccttcag cctgacgatc atgagcggcc ggctcgacaa
     1261 gtcgatcgcc agccagcagc gcttcatgaa ggacgcgcgc cgcgcgatcg agtggctcaa
     1321 ccgcgaactg cgcgtcgcca cgaccgacgt catgtccccc atcagcgtct acgatgacga
     1381 cggcgacgcg gcggtgcgcg gcaaccgcgt cgagttctcc cgctcgggcg aggccgccgg
     1441 cctgcgcgcc ttttacctcg agtccgacga cgacgacatg atgaccccct gggacaatcg
     1501 gctgttctac gaccccaaca ccggcgccgc cggcgacgag atcctggtca gccgcgacat
     1561 cgccccggtc aatcccaatg gcgccttcca atacgagggc gccagcgaac ccgtggtgat
     1621 ccagatgcgg accggcgacc cggccaattc gaccaacgag caggaggact gggccaactc
     1681 gggcgccaac ctgcagggcg ttgaaatcaa catccgcatc gcgccgcgca actgacgcgc
     1741 tcgctgacgg caggacgccg atccttggac cctttcgaga ggacccccgc catgcaacgc
     1801 aaaatcaacc gactccttcg ctcaagcggc ccgcgccgcg ggtcggccct cgtgctcgtc
     1861 ctgatcagct tcatgctgct gatgatgatg tcgggcggcg tgctgctcgt ctcgacccag
     1921 cagacgcgcg accgcgtccg ttacgaagcc tacaaggacg aattcgcggc ggccgaactg
     1981 gccatcaaca aagccttcgg ccatatcgtc ttcatggtgc gcaacggcac gcccgatctg
     2041 ctcgacgaga tcgacaatat taatgcgccg gtgctcgacg gctacgattt ccccgcgttc
     2101 gaggttgccg agacattcaa cggcaatgag acggttcccg aaggcgagaa gtgggccggc
     2161 ctcaacctcc accgcgagcg ctactccatc gacgtcacgg cggtcaagac cggcggctcc
     2221 gccgaccggt tcgaacgccc cggcgtcagc atgacgcaga acctcgaaat cacctacatc
     2281 ccgctctacc ttttcgcgat tttctacgac ccgctgatgg agattgcccc cggcccgccg
     2341 atggtcgtca acggcctggt ccacgccaac ggcgatgcct atatccaggc caacgaaaag
     2401 ctcgattttc agaagaacgt ctcggtcgcc ggccggttct accatggacg ggccccgggc
     2461 tccggccagt ccgatagcag cggcaccgtc cgcttcacca agccgcccga tacgctcgtc
     2521 agcatgctgc aaggcggaag ctggctcgac agccgcctgt cgaactgggc gtcgctcgcg
     2581 accgcgacct ggggcggcgg gctgcgcgac aagtcccacg gcgccccgca gctcaatctg
     2641 ccgatccccg acggcgtcaa tccctacgcc ctcatcgaac gcgagtcggt cagcgacggc
     2701 ttcgccttgc agaatgagaa gttcgagcgc aaggccggct tgaagatcct cgccgactcc
     2761 gccggcaacg tcaccgccgt cgacaaatac ggcaatccgg tcaatctgtg gtatcccgat
     2821 cccggcaacc ccactctgaa gaaatggatc tacagcaaga cgacgttcta cgacgcccgc
     2881 gaaggcaaga ccgtcacctc gatcgacctg aatctgggca acatggtcga ggggggcatc
     2941 atgccctcca acggcatcct ctatgtctcc aacgaggatc agtcgggcaa accgggcgtc
     3001 gttcggctca ccaacggcgc caagctcccg cagtcgggcg tcgtcgaggg cttcaccgtc
     3061 gccaccgatg acccgctcta catcaagggc gactacaaca ccgtcaacaa gaccctcgcc
     3121 atggtcgcct gcgattcgct gatgatcctc tccaacgcct ggaacgatgc caactcaacc
     3181 aattacagca accgcatcgc ctcggagacg accgtcaaca ccgtctgcat gcagggcaat
     3241 gtccccaccg gcggcggcta ttacagcggc ggcgtcgaga attacttccg cttcctggag
     3301 aactggagcg gcaaggatct caacttcagc ggctcgatca tcctgatgtg ggccagccag
     3361 aagttcctcg gcaaatgggg ccagagcaac gtctattcgc cgcccaaccg caagtggtcg
     3421 tgggatacgg cgctgggggg catcaacggg ccgcccggcg ccccgcgcgt cgtcgagctg
     3481 gagaagaaag actggaaaat ggccagcgcc gactgagccg ccaatccccc gcaaaaatcc
     3541 aagccccggt cgccaatctt cgcgaccggg gctttcactc gtacgggccg gctattgcag
     3601 cagcttcagc cggctcaccc ggtgccgttc ggcatatagg atccgccgca ccttctcgac
     3661 cgactcgtcg atatcctcgt tgacgaccag atagttgtat tggcgccagt agatcatctc
     3721 gcggcgggcg ttttccaggc gcatgcggat cgcctcctcg ctgtcggtcg cccgctgccg
     3781 cagccgctgc tccagcacct gaaacgacgg cggcatcagg aacaccagga cggcatcggg
     3841 cgagcgatac ttcgccgaca accccccctg cacgtccagg tcgagcgcta cgtcgcgccc
     3901 ctccgccagc gccccctcca ccgtgtccga ccgcgtccca tacaggttgc cgtggacctc
     3961 cgcccactca tagaagacat cctgctgcac gagcgactcg aattcggccc gcgagacgaa
     4021 gtggtagtcc cgcccctcga cctcgtcggg gcgcggcgcc cgcgacgtca ccgagaccga
     4081 atagtgcaga tcggcctcgc tggccagcag gcgcgtgagc acggccgact tcccgccgcc
     4141 cgatggggcg gagatgacga acaacagacc gcggcgttgg aatcggtaga gagacacggg
     4201 ctcaatcttt cacgataggt tggcgtgccg acacgcgccg ccgggaacgg gcccttttcc
     4261 cggacttggc ttggctcccc cgctggccgg cctcttcctt tttcaccagc cggatctcaa
     4321 cccggtaacc catcgcctcg gcgtagcgcc gcagcgtcgc cagcgagggc gaatgtttcc
     4381 ccgccggcga ttcgagccgc gcgatcgccg actgcgtcgt accgatcttc cgcgcgacat
     4441 cggcttgact catccccgac tcgcggcgcg ccttgaggaa ttcatcgaac aacgcgaatt
     4501 cctcctccag cttgtcatac tcgcgccgca cctcgggccg cgacagcatc cgcgtcctca
     4561 gccggtcatc ggcgcctcgc atgtttcacc tcggtcattc gcgaacgggc gatttccaga
     4621 tgccggcgcg gggttttgcc gctcttcttc aggaactgat ggagaaacac gattcgtcgc
     4681 ccgatcaatg tgcaatgaaa gatccggacc atgccttcgc ccgatttgag ccgcaactca
     4741 aactacccct gcccagggcg cgtgtgtggg gcatccccag agcagggccc tcgcgctgca
     4801 tccgatcaac gcagtgcacg aagcgggcgg ctatccccgc cggcaatcgt tagatttctt
     4861 cctccagctt ttcactatgg aaatggaccg tccagccggt catttaacat ctccgctatg
     4921 caggggcaag gcgcgaaccg gtcctacccc atctgcttca caatcgcctc cgcaaagccc
     4981 gatgtcgaga ctttcgtcgc gccgctgatc tggcgctcca ggtcgtaggt caccgtcttg
     5041 gcggcgatcg ccccctcgat cccgcggatc accagctcgg ccgcctcgtt ccaccccaga
     5101 tgctccagca tcatcaccgc cgacaggatc agcgatcccg ggttgaccat gttcttgccg
     5161 gcgtatttcg gcgcggtgcc gtgcgtcgcc tcgaagatcg cggcgttgtc gccgatgttc
     5221 gcccccggcg ccatccccag gccgcccacc tgcgcggccg ccgcgtccga gatgtagtcc
     5281 ccgttcaggt tcatcgtcgc cagcaccgag tactcatccg gccgcagcag gatctgctgg
     5341 aagatcgagt cggcgatccg gtccttgatc agcaccttgc ccgccggcat cttgccgtcg
     5401 tattcgctgt aaagctcatc ctccgtcacg atctgttcgc ggaagttctc cttcgcctcc
     5461 tcataccccc attcgcggaa cgccccttcg gtgtacttca tgatgttgcc cttgtggacc
     5521 agcgtcaccg acggcagc
//
LOCUS       DAOPCO010000264         5537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12614_length_5537_cov_4.689347, whole genome shotgun sequence.
ACCESSION   DAOPCO010000264 DAOPCO010000000
VERSION     DAOPCO010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12614_length_5537_cov_4.689347"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLS90_14330"
     CDS             complement(<1..371)
                     /locus_tag="PLS90_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03621.1"
                     /translation="MREWSEFKTVGDARPKPARGRVGAWVSMICLVLFLSTVGLLVVV
                     LLVRLIPLLILASVFGWGYTRRSAEGLSRLQLGRGDWGMLFCLMFALWGIALARAVLV
                     GWWGEAPGNLATFGLINVAVAV"
     gene            570..1607
                     /gene="queG"
                     /locus_tag="PLS90_14335"
     CDS             570..1607
                     /gene="queG"
                     /locus_tag="PLS90_14335"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPK03622.1"
                     /translation="MVRVQANTEARELRERIRAEALTVGFSRAGFTGAEPLGPTIERR
                     RTEWLAEGRAGAMQYLHREHPRRTHPRDLLPEARTVLVVLAGYYDGDHPPAAPDEGRI
                     ARYAWGEDYHGVMRRRLDALAEGIQRLAQGAGIAAPLRVRPCVDSAPIDERALAARAG
                     LGFIGKNTLLIDPAGGSWTLIGVLLMSLDLPPDEPLRGPAASCGGCRRCLDACPTGAI
                     DRPYRLDARRCLSYLTIEQKDEIPLELAARMEGWAFGCDICQEVCPFNDRPLGRLLPE
                     LAADRGAGPLATPAMLERDPSARAFRRRWGHTPLERPGRKGLERNLRALAAGRRPVPA
                     GAMRPSQEDPS"
     gene            1604..2095
                     /locus_tag="PLS90_14340"
     CDS             1604..2095
                     /locus_tag="PLS90_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03623.1"
                     /translation="MRVLPSRRRLLWRVAFLVVLLLLMAQSLRVINLEQRHRDERIEA
                     DGLLAEARQRALRDDLVATSAAAELERRAPDGWGDAGQIVRRLRDDLLQRPDLIGLPG
                     VLGGRMAFRPDDVHVLNDRWVLAGYEDGHISGWGLFEFSFAGEEIHWRPVESSADEPE
                     EPR"
     gene            complement(2153..2506)
                     /locus_tag="PLS90_14345"
     CDS             complement(2153..2506)
                     /locus_tag="PLS90_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPK03624.1"
                     /translation="MSIFNVTPAKERELLSRMAACGLREADLEEHFIRGGGPGGQKIN
                     KTASAVRLRHRPTGLEVKMQRTRSRALNRFHARRRLCELLEAERLGAASPAAVEATRR
                     RKQKDRRQRRAREAE"
     gene            2702..3832
                     /locus_tag="PLS90_14350"
     CDS             2702..3832
                     /locus_tag="PLS90_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPK03625.1"
                     /translation="MKIITRYLLRDIFHYFWIFLVMFVVLLLIYELYDMRGEFLEKDP
                     SLTDIILYLVFVIPSQLSEMLPVIALLSSIFAYGLLAKNREILAMVAAGVSFRFLAFP
                     ALVFGLLVTAAAYGFNETVVPYTESRARYLEKVTIGQKDPRRFTQRDDVLVRGAGNSI
                     YFIERYYSDRAEMRFVTLWSISDTGDRPTERIDAVHAVRVEPENQQGKPEVSLWELHG
                     AERRTFDENGRLLRYERIPGAYRIPLEANLDLYLSQSKKPEEMTIAELQEYYEFLEAK
                     EGQDMLIFGMTIQRKLSFPLACLLMALLGFGVVADVHARRFARGVFAGILTAVAFYVF
                     SAFMTNLGEGGVLMPYAAAWLPIFVFALVVYLFYTRLNAIRG"
     gene            3846..4619
                     /locus_tag="PLS90_14355"
     CDS             3846..4619
                     /locus_tag="PLS90_14355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK03626.1"
                     /translation="MAPSRAIDRYFGPAYQRMYTRYLLPPQQTAAEAEYLLGRLRPKA
                     GDRWLDIPCGYGRHLAELRRLRPRLRLAGCDLNRDYLAAARQPHLALARADYRRLPWA
                     AGSFHAVLMLLNSFGYYPPRGREHPARPDDRAVLREAARVLRPGGWLVLDLSNRRSLL
                     ELVRRQPRIRYGGGEYDVREEFGWDAAGQCLTNRTRWTWPGGRQTAGYRLRLYTPAEI
                     RRLLPRAGLQWVEAVGSFRGEPFDPIHSERMIVLARRER"
BASE COUNT          877 a         2007 c         1726 g          927 t
ORIGIN      
        1 acggcgacgg cgacgttgat cagcccgaac gtggccagat tgccgggagc ttcaccccac
       61 cagcccacca gcaccgcgcg cgccagcgca atcccccaca gcgcgaacat caggcagaac
      121 agcatccccc agtcgccccg gccgagctgc aggcgcgaca gcccctccgc gctgcgccgc
      181 gtataccccc agccgaagac cgaggcgagg atcagcagcg ggatcagccg caccagcagc
      241 acgacgacca gcagccccac cgtggagaga aacaggacga ggcagatcat tgaaacccac
      301 gcgccgaccc gtccgcgcgc cggtttcggc cgggcatcgc cgaccgtctt gaactcgctc
      361 cattcgcgca tgggttcatc cctccgcggg agctcgcacc ttcgtcaccg gaagacgcgg
      421 aaaacgccgc tgcgatccac cgggtattat ggaacgaaaa caatcagcac tcgcaagcgc
      481 catcggtatc gatatcgcca ccgcctcgct ccctcccgcg ccgccccctc gccgttgttg
      541 cttgtcggcg atgttgctac aatcatccca tggtgcgcgt gcaggccaac accgaggcgc
      601 gggaactccg cgaacggatt cgcgcggaag ccctcaccgt gggattttcg cgcgccggct
      661 ttaccggcgc cgaacccctc ggcccgacga ttgaacggcg ccggaccgaa tggctcgccg
      721 aaggacgcgc cggcgccatg cagtatctgc atcgcgagca cccgcgccgg acgcacccgc
      781 gcgatctgct gcccgaagcc cgcaccgtac tcgtcgtcct ggccggatat tacgacggcg
      841 accatccccc cgccgccccg gacgaagggc ggatcgcccg ttacgcctgg ggcgaggact
      901 accacggcgt tatgcgccgg cggctcgacg cgctggcgga agggatccag cggctcgcac
      961 agggcgcggg catcgcggcg ccgctccggg tgcgcccatg cgtggacagc gcgccgatcg
     1021 acgaacgggc gctcgccgcc cgcgccggct tgggcttcat cggcaagaac accctgctca
     1081 tcgatccggc gggggggtcc tggacgctca tcggcgtgct gctgatgtcg ctcgacttgc
     1141 cgcccgacga accgctgcgc ggccccgcag cgagctgcgg gggctgccgg cgctgcctgg
     1201 atgcgtgccc gaccggcgcg atcgaccgcc cctaccgcct cgacgcgcgc cgctgcctct
     1261 cctatctgac gatcgagcag aaggacgaga tccccctgga gttggccgcg cggatggagg
     1321 gctgggcgtt cggctgcgac atctgccagg aggtctgccc gttcaacgac cggccgttgg
     1381 ggcggctgtt gcccgagctc gccgccgacc ggggcgccgg accgctggcg acaccggcga
     1441 tgctggaacg cgacccgagc gccagagcgt tccgccgccg ctgggggcac acgcccttgg
     1501 aacgccccgg ccgcaaggga ctggaacgca acctgcgggc gttggcggcg gggcgtcgac
     1561 ccgtgccggc cggcgcgatg cgcccgagcc aggaggaccc gtcatgagag tgctgccctc
     1621 gcgccgccgc ctgctctggc gcgtcgcgtt cctcgttgtc ctgctgctgc tgatggcgca
     1681 gagcctgcgc gtcatcaacc tggaacagcg ccaccgcgac gaacggatcg aagccgatgg
     1741 gttgctggcc gaagcgcgcc agcgcgcgct gcgcgatgat ctggtggcca cgtcggccgc
     1801 cgccgaactc gaacggcgcg ccccggacgg ctggggcgac gccgggcaga tcgtgcgccg
     1861 cctgcgcgac gatctgctcc agcggcccga ccttatcggc ctcccgggcg tcctcggcgg
     1921 acggatggcc ttccggcccg atgatgtgca cgtgctcaac gaccgctggg tgctggccgg
     1981 ctacgaggac gggcacatct ccggctgggg acttttcgaa ttttcgttcg cgggcgagga
     2041 gatccactgg cgcccggttg aatcctcggc ggacgaaccc gaggaaccac gctgatcaaa
     2101 aaaccgtctc tcgtcctcgt ccctcgtcct cgaagttgcc acgccacccc cgtcactccg
     2161 cctcccgtgc ccggcgctgg cggcggtcct tctgtttgcg gcggcgcgtc gcttccaccg
     2221 ccgccgggct ggccgcgccc agccgctccg cctcgagcag ctcgcacagg cgccgccgtg
     2281 catgaaaccg gttcagcgcc cgcgatcgcg tccgctgcat cttgacctcc agccccgtcg
     2341 gccggtgccg cagccgcacg gccgaggcgg tcttgttaat cttctggccg ccggggccgc
     2401 ccccccggat gaaatgctcc tccaggtcgg cttcgcgcag cccgcacgcc gccatccggc
     2461 tcaacagttc gcgttccttg gcgggggtca cgttgaagat cgacatcgcc cgatccgaat
     2521 cctcgactgg ctccccatcg tatcgcgcct ccgcgccgcc gcaacccccg ttttggcttg
     2581 ccgccgtgcg ggggggccga tatggtatta gccgaccgcg cccgcttgtc gcgtcgacgc
     2641 cgagccggct tcctccgatc cagtgctccg ggcgccttgc gcccccaccg tccggcccgc
     2701 catgaagatc atcacccggt acctgctgcg cgacatcttt cattatttct ggatcttcct
     2761 ggtcatgttc gtcgtgctgc tgctgatcta cgagctgtac gacatgcgcg gcgaattcct
     2821 ggaaaaagat ccctcgctca ccgacatcat cctctatctg gtcttcgtca tcccctcgca
     2881 gctcagcgag atgctccccg tcatcgccct gctctcgtcg atctttgcct acgggctgct
     2941 ggcgaagaac cgcgagatcc tggcgatggt ggccgccggc gtcagcttcc gcttcctggc
     3001 gttcccggcg ctggtgttcg gcctgctggt gaccgctgcc gcctacggct tcaacgagac
     3061 ggtcgtcccc tacaccgaaa gccgggcgcg ctaccttgaa aaagtaacga tcggccagaa
     3121 ggacccgcgc cgcttcaccc agcgcgatga cgtgctggtg cgcggcgccg gcaactcgat
     3181 ctatttcatc gagcgctact actccgaccg cgcggagatg cgcttcgtca cgctctggtc
     3241 gatcagcgac acgggcgacc ggcccaccga acggatcgac gccgtccacg ccgtgcgcgt
     3301 cgaaccggaa aaccagcagg gcaaaccgga ggtcagtctg tgggagctgc acggcgccga
     3361 acgccgcaca ttcgatgaaa acggccgtct gctccgctac gagcgcatcc cgggggcgta
     3421 tcgcatcccg ctggaggcca acctggatct ctacctgagc cagtccaaga aacccgagga
     3481 gatgacgatc gccgagctgc aggaatatta cgagttcctc gaagcgaagg aggggcagga
     3541 catgctgatc tttggcatga cgatccagcg caagctctcc ttcccgctgg cctgtctgct
     3601 gatggccctg ctgggcttcg gcgtcgtcgc cgacgtccac gcgcggcggt tcgcccgcgg
     3661 cgtcttcgcc ggcatcctga ccgccgtcgc cttctacgtg ttcagcgcgt tcatgaccaa
     3721 cctgggcgag gggggcgtcc tgatgcccta tgcggcggcg tggctgccga tcttcgtctt
     3781 cgccctcgtc gtctacctct tctatacccg cctcaacgcc atccgcgggt aaagccgggt
     3841 cgcccgtggc gccgtcgcga gccatcgacc gctatttcgg cccagcctat cagcggatgt
     3901 acacacgcta tctgctgccg ccgcagcaga cggccgccga ggccgaatac ctcctcgggc
     3961 ggctgcgccc gaaggccggc gaccgctggc tggacatccc ctgcggctac ggccgccacc
     4021 tggccgagct gcggcgcctg cggccgcgcc tgcggctggc cggctgcgac ctgaaccgcg
     4081 actacctggc ggccgcgcgc caaccccacc tggccctcgc ccgcgccgat taccgccggc
     4141 tcccctgggc cgccggcagc tttcacgccg tgctgatgct gctcaacagc ttcggttact
     4201 atcccccgcg cggacgcgag catcccgcgc gccccgacga ccgcgcggtc ctgcgcgaag
     4261 cggcccgcgt cctgcgcccg ggcggctggc tcgtgctcga tctgagcaat cgccgcagcc
     4321 tgctggaact ggttcgccgc caaccccgga tccgctatgg cggcggcgaa tacgacgtcc
     4381 gcgaggagtt cggctgggac gccgccggcc agtgcctgac caaccgcacg cgctggacct
     4441 ggccgggggg acgccagacg gccggctacc gcctgcgcct ctatacgccg gccgagatcc
     4501 ggcggctgct gccgcgggcg ggcctgcaat gggtcgaggc cgtcggctcg ttccgcggcg
     4561 agccctttga ccccattcac agcgagcgca tgattgtcct cgcccggcgc gaaagataac
     4621 ttgggatgat tggtcccttt aggtcccttc cgtccctaat gccccttgcc cttgggctcc
     4681 ccccaaaaaa tcaacattat taacttattt tcggccatcc ccctcccagc gcctgctcgc
     4741 agcaaagatt tcatttgcac gtcacggtcc gttgtgctct aatgaccatc actgatggcc
     4801 cccctgaacg cactggtatc acaaatttct cgaggtagtc tgactcactg aactgatttt
     4861 ggacgtggcg cgaccctgac tggcgcgccc gcatttcgcc gctttggaga cagcttcgcc
     4921 gacgtcacca gcccgactca ggctcacggc cccgccctcc ggcgggcgtg cccgcgaggt
     4981 gaatcgaacg gacgcgtcgc gacgctcaat ccagacccgg cgagatgtcc actatcgggt
     5041 tcgggagttg tccgttgacg gcgcctgccg ataggttcct tggcgggact tcggccgaag
     5101 ctccggcgtg cgcgcgttgt cttggacgcg ttgacgacga cgcgcgacga tttgtggaga
     5161 aaaatgaggg ttgacaccca cggtcgccct ctatatagaa ctaaaggcat aggcagacga
     5221 ctcaaagcca acccagcgac caggtaactg gccggcctca cgcaaaaggg cgcgcgatcg
     5281 gggagaggcg gcatgaaatg ggatcgacgg cggcggtgtc tggtttacag taaagttcgc
     5341 agccaggcgt gaggccttct ccccgcgcgg gctgatacca accatcagaa ggagctgtca
     5401 gaccatctgc atgattcgca ttttaatgtg ttgccttatt tctcactcat aaggctcacg
     5461 actttacgga aaggagagag ggaagccgta tgcgaaaagg gttcaaactg accctgatgg
     5521 ttgctatcgc ctttatg
//
LOCUS       DAOPCO010000265         5516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12665_length_5516_cov_4.586339, whole genome shotgun sequence.
ACCESSION   DAOPCO010000265 DAOPCO010000000
VERSION     DAOPCO010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12665_length_5516_cov_4.586339"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLS90_14360"
     CDS             <1..546
                     /locus_tag="PLS90_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03627.1"
                     /translation="ALLLVPWLMLAAGCLLIGLSFRFWKPMVGIGGGPCRSAKFIRAA
                     WGWLALSLGMLLLLPAYQAASGIPFSHAYYGAIRHAITVGFVSLMIMGIAARIVPMLA
                     DVDHRTLPGLWAPFVLVNLGCLLRVSTQTLTNWHPLFFGVIGVSGMLEVAGLALWGAH
                     LAAVMLGSRSLSVFVARPRLS"
     gene            737..2674
                     /gene="mnmG"
                     /locus_tag="PLS90_14365"
     CDS             737..2674
                     /gene="mnmG"
                     /locus_tag="PLS90_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPK03628.1"
                     /translation="MTAEHYDIIVIGAGHAGCEAAHAAARLGRRVLLLTLDPRHVALM
                     SCNPSIGGLAKGHLVREIDALGGVQAQATDATGIQFRMLNTRKGPAVQAPRAQCDKLA
                     YNAWMRDRMAATPGLTVAAGMAVDLLLERDGAGGCRVAGVLVDTGGGEPERVGARAVI
                     CTTGTFLDGLIHIGLRREPAGRMGERAAVGLGDALQRIGFETGRLKTGTPPRLRAASI
                     DWARFEEQPGDEPIPAFSFTSGAITRRQASCWIGFTTERTHEIIRGGLDRSPLYTGVI
                     QSIGPRYCPSIEDKVVRFADKARHQIFLEPEGLTTDWIYPNGLSTSLPEEVQDAMLRS
                     IPGLERVEMIRPGYAVEYTYCPPLQLYPSLETRLVAGLFFAGQINGTSGYEEAAAQGI
                     IAGINAALRLRGEEPLVLRRDEAYIGVLIDDLITMEHREPYRMFTSRAEYRLLLRHDT
                     ADLRLTPHGRRVGLVDDARWRRFESFRARVEALRDQVGERHLRPDDAGRERFEAAGLP
                     WPEKPTTIRQYLARPEVTLAAAEAAGLLADAAAAGGEGDPAAALDARRVRHEVLLRIK
                     YEGYIAKQREQVERMLRAEAKPLPEWVDYDAVRGLRREAQDKLKRFRPATVGQALRIA
                     GINATDMSLVLVHLRGRERAA"
     gene            complement(2705..3499)
                     /locus_tag="PLS90_14370"
     CDS             complement(2705..3499)
                     /locus_tag="PLS90_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HPK03629.1"
                     /translation="MTSTRIEWTQSVWNPVVGCSKVSSGCKNCYAERMARRLVAIAAA
                     ARARGDDPGRAGNYQKVLNANGNWSGGIFLDDLSLHAPYGWRQPRVVFVNSMSDLFHE
                     NVPFDFIRKVFNVMNECESHTFQVLTKRPERAALFSGKLGWTSNIWIGTSVENDEVSG
                     RIDHLRKVGGCVRFLSLEPLLGPLPNLNLTDIDWVIVGGESGPGARKMRPEWVHSIQQ
                     LCRANGVPFFFKQWGGANKKRNGRLLGGKTYDEMPDLAQVHRVRAA"
     gene            complement(3496..4614)
                     /gene="tcmP"
                     /locus_tag="PLS90_14375"
     CDS             complement(3496..4614)
                     /gene="tcmP"
                     /locus_tag="PLS90_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HPK03630.1"
                     /translation="MARDINKNPYDSKTLTKLEIFEAYVREWLPVFIESRHVTRAAIW
                     DFFAGSGRDSDGVPGSPLRILNQIESFKTKLAAKPNMQIRVVLNEGNSRKYADLSRVV
                     EEGRGSWDLTDKVSVECRNADFQDLFRLCYPELMRQPNLILIDQYGIKHVNRDVFHML
                     IGLPKTDFLFFISSSYIKRFARTHEFSRIFPDMQSFQLADARAQDVHRIVLEYYRSLL
                     PSTSATRLYPFTLRRGERSGNVYGLIFGSAHPHGVEKFLDVAWNRNRINGEANFDIDD
                     DLEKLEPVFSDEFRRFTKRECFERDLVSFIHDAGERTNAEVYEFTLARGHPKAHAMEC
                     LRRLRREKKIDCPANFGISYKCCVSEHRRIVTVRSLSR"
     gene            complement(4797..>5516)
                     /gene="amrB"
                     /locus_tag="PLS90_14380"
     CDS             complement(4797..>5516)
                     /gene="amrB"
                     /locus_tag="PLS90_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPK03631.1"
                     /translation="DRPPRLYIILGVAHSPTRNLFTLTDKSFATPLGLVETDRDAAAR
                     LRELYGAERLSGQIAHRLEHSIEFTTVGLRYLHGASHNGSPGGTVRMLPLLCGSLHEV
                     LEEDGLSPRELPVVNDFIEALRRLIDELNGDVCLIASVDLSHVGLKFGDAEGISDLRR
                     ELVRSADDAMLATIERGEPEAFFDTFRADRNARNVDAITSVYVLLHALGAVARAERLD
                     YQQWHEAATDSMVSYASLALY"
BASE COUNT          987 a         1605 c         1874 g         1050 t
ORIGIN      
        1 gcgctgctgc ttgtgccgtg gctgatgctg gcggcggggt gcctgctgat cgggctgtcg
       61 tttcgcttct ggaagccgat ggtggggatc ggcggcgggc cgtgccggtc ggccaagttc
      121 atccgcgcgg cgtggggctg gctggccctc tccctgggca tgctgctgct gctgccggcg
      181 tatcaggcgg cgagcggcat tccgttcagc catgcgtact acggggcgat ccgccacgcg
      241 atcacggtcg ggtttgtctc gctgatgatc atgggcatcg cggcgcggat cgtgccgatg
      301 ctggccgacg tggaccaccg gacgctgccg ggattgtggg cgccgttcgt gctggtgaac
      361 ctgggctgcc tgctgcgtgt ctcaacccag acgctcacga actggcatcc gctgttcttc
      421 ggtgtgatcg gcgtcagcgg gatgctggaa gtggccggcc tggcgctgtg gggagcgcat
      481 ctggcggcgg tgatgcttgg ttctcgaagt ctgtcggtgt tcgtcgctcg gccgcggctt
      541 tcctgagcag gcaagggacg taaaggacgt aagggacatg cagatgggcg gccggggacg
      601 cagttcctgg tgagccgttc atgccgctcc gcgcggcgtc gagggtcggc tggctggcgt
      661 ccggatcttt cacatcccgc cctgaccgga tataatgccg gatcattttc gcccatggcg
      721 gcggcgggat gtgaggatga cggcggagca ctatgacatc atcgtgatcg gggccgggca
      781 tgccggctgc gaggcggcgc acgcggcggc gcggctgggc cggcgcgtcc tgctgctgac
      841 gctggacccg cggcatgtcg cgctgatgtc gtgcaacccg tcgattggcg ggctggccaa
      901 ggggcacctg gtgcgcgaga tcgacgcgct ggggggcgtg caggcgcagg cgaccgacgc
      961 gaccggcatt cagttccgca tgctgaacac gcgcaagggg cccgccgtgc aggcgccgcg
     1021 ggcgcaatgc gacaagctgg cctacaatgc ctggatgcgc gaccggatgg ccgccacgcc
     1081 gggtctgacc gtggcggcgg gcatggccgt cgatctgctg ctggagcgcg acggcgcggg
     1141 ggggtgccgg gtggctggcg tgctggtcga cacgggcggc ggcgagccgg agcgcgtcgg
     1201 cgcgcgcgcc gtcatctgca ccaccggcac gttcctggac ggcctgatcc acattggcct
     1261 gcgccgcgag ccggcgggcc gcatgggcga acgcgcggcg gtggggctgg gcgacgcgct
     1321 gcagcgaatc ggcttcgaga cggggcggct caagacgggc acgccgccgc ggctgcgcgc
     1381 cgcgtcgatc gactgggcgc gattcgaaga gcagccgggc gacgagccga tccccgcctt
     1441 ttcgttcaca agcggcgcga tcacgcggcg gcaggcgagc tgctggatcg gcttcaccac
     1501 cgagcgcacg cacgagatca tccgcggcgg gctggaccgc tcgccgctct ataccggcgt
     1561 gatccagagc atcggaccgc gctactgccc gtcgatcgag gacaaggtcg tgcggttcgc
     1621 cgacaaggcg cgtcatcaga ttttcctgga accggagggc ttgacgaccg actggatcta
     1681 ccccaacggg ctgtcgacgt cgctgccgga ggaagtgcag gacgcgatgc tgcgctcgat
     1741 tccggggctg gagcgagtcg agatgatccg gccgggctat gcggtggaat atacgtattg
     1801 cccgccgttg cagctttacc cgtcgctgga gacgcgcctg gtggcggggc tgtttttcgc
     1861 cgggcagatc aacggcacgt cgggctacga ggaggcggcc gcgcagggga tcatcgccgg
     1921 gatcaacgcc gcgctgcggc tgcgcgggga ggagccgctc gtgctgcggc gcgacgaagc
     1981 ctacatcggt gtgttgatcg acgacctgat cacgatggag caccgcgagc cgtaccggat
     2041 gttcacgtcg cgggcggagt atcgcctgct gctgcgccac gacacggccg acctgcggct
     2101 gacgccccac ggccggcggg tggggctggt ggatgacgcc cgctggcggc ggttcgagtc
     2161 gtttcgcgcg cgggtggagg cgctgcgcga ccaggtcggc gagcggcatc tgcggcccga
     2221 cgatgcggga cgcgagcgat tcgaggcggc cggactcccc tggccggaga agccgacgac
     2281 gatccggcag tatctggcgc ggccggaggt gacgctcgcg gcggcggagg cggcggggct
     2341 gctggccgac gccgcggcgg cgggggggga aggcgacccc gcggcggcgc tcgatgcccg
     2401 acgtgtccgg catgaagtgc tgctgcggat caagtatgag ggttacatcg ccaagcagcg
     2461 agaacaggtg gagcggatgc tgcgcgccga ggcgaagccg ttgcccgagt gggtcgacta
     2521 tgacgcggtg cgggggctgc ggcgcgaggc gcaggacaag ctgaagcgtt tccgtccggc
     2581 gacggtgggt caggcgctgc gcatcgcggg gatcaatgcc accgacatga gcctggtgct
     2641 ggttcacctg cgcggccggg aacgggcggc gtaagggcgt agagattgaa gatcctgcac
     2701 cagactatgc cgcacgaacg cgatgcactt gagccagatc gggcatttcg tcatacgtct
     2761 ttccgccaag cagccggcca tttctcttct tattagcccc gccccactgc ttgaaaaaga
     2821 agggcactcc gttggcgcga cacagctgct gaatcgaatg cacccattcc ggcctcattt
     2881 ttcttgcgcc gggtccagat tccccaccga caatcaccca gtcgatatcg gtcaaattga
     2941 gattcggcaa cggcccaagg agtggttcaa gagacagaaa tcggacacat ccacccactt
     3001 tgcgcaggtg atcgatgcgt cccgagactt catcgttttc aacgctcgtt ccaatccaga
     3061 tgttcgaggt ccagcctagc ttacctgaaa acagagccgc gcgctcgggg cgcttagtca
     3121 gcacttgaaa tgtgtgcgat tcgcattcat tcatgacgtt gaaaactttt cggatgaagt
     3181 cgaacgggac gttttcatgg aacagatcgc tcattgaatt gacaaagacg acgcgcggct
     3241 gacgccaccc atacggcgcg tgcaagctga ggtcatccag gaaaatcccc ccactccaat
     3301 taccgtttgc attcaagacc ttctgataat ttcccgctcg cccggggtca tcgccacgcg
     3361 cgcgcgcggc ggcggcgatc gcgaccagtc gccgggccat ccgctcggcg tagcagttct
     3421 tgcaccccga gctaaccttc gagcagccaa ccacggggtt ccaaacggac tgggtccatt
     3481 cgatcctggt gctggtcatc tggataggga cctcacggtc acgattcggc gatgctctga
     3541 gacgcagcac ttgtagctta tcccaaagtt ggcggggcaa tcgatcttct tttcgcggcg
     3601 aagccttctc aggcattcca tggcatgggc cttgggatgt ccgcgcgcca aggtaaactc
     3661 atacacctcg gcatttgttc tttctccggc gtcatggatg aaggacacaa ggtctcgctc
     3721 gaagcattct cgtttcgtga accgccgaaa ttcatcggag aacacgggtt caagtttttc
     3781 caggtcgtca tcaatatcaa aattggcttc gccgttgatg cggtttctgt tccacgcaac
     3841 gtcgagaaat ttttccacgc catgcggatg ggcgctgcca aatatcaaac cgtatacatt
     3901 cccggaccgc tctccacggc gcagcgtaaa tgggtacaat cgagttgcgc ttgtcgaggg
     3961 aagcaaactc cgatagtact caagcacgat ccggtggaca tcttgtgctc tagcgtccgc
     4021 gagttgaaaa gattgcatgt ctggaaagat acgtgagaat tcatgggttc gagcgaatcg
     4081 cttgatatat gatgaggaaa taaagaacag aaaatcggtt tttggcaagc caatgagcat
     4141 atggaataca tcacggttta cgtgttttat tccatattgg tctatcagga tcaagttagg
     4201 ctgccgcatt agttcggggt aacacaggcg gaataaatcc tggaagtcgg cattgcggca
     4261 ttccacagac actttgtccg tcaggtccca tgaaccccga ccttcttcca cgaccctcga
     4321 caagtcagcg tatttccgtg aattgccttc gtttagaacg acacggatct gcatatttgg
     4381 cttcgcggca agttttgtct tgaaggactc aatttggttt agaatccgca atgggctgcc
     4441 cggcacaccg tcggagtcgc gaccggaacc ggcgaaaaaa tcccagattg cggcacgggt
     4501 tacatgtcga gactcgatga ataccggcag ccattctcta acgtatgcct cgaatatttc
     4561 caactttgtt aaagttttcg agtcataggg attcttattg atgtctcggg ccatcatgga
     4621 actccatact ggcgcgtcag ccgatgcgaa cctgatccag ccgggctaac ccgaaggaat
     4681 gccagatggc tttggaaaac tcaatatatt tatggatgaa aatatctcaa tagttggctg
     4741 cgccagcact gctgtcgcag ttcagcttgg atgtaagccg tccattgggg ggcgggtcag
     4801 tagagcgcga ggctggcgta gctgaccatc gaatcggtcg cggcctcgtg ccattgctgg
     4861 tagtcgaggc gttcggcgcg ggcgaccgcg cccagggcgt ggagcaggac gtagaccgac
     4921 gtgatcgcat cgacattgcg cgcgttgcga tcggcgcgga aagtgtcgaa gaaagcttcg
     4981 ggttcgcccc gttcgatggt ggcgagcatg gcgtcatcgg ccgaccggac aagctcgcgg
     5041 cgcagatcgg agatcccctc ggcgtcgccg aacttcaggc cgacgtgact cagatcgacg
     5101 ctggcaatca gacagacatc gccgttgagt tcgtcgatca gccggcgcag cgcttcgatg
     5161 aaatcgttga ccaccggcag ctcgcgcggc gagaggccgt cctcttccag aacttcgtgg
     5221 agcgagccgc aaagcagggg gagcatccgc acggttccgc ccggcgagcc gttatgactg
     5281 gcgccgtgca ggtaacgcag gccgacggtg gtgaactcga tgctgtgctc gagccggtgg
     5341 gcgatctgcc cgctgaggcg ttcggcgccg tagagctcgc gcaggcgcgc cgcggcgtcg
     5401 cggtcggtct cgaccaggcc gagcggggtg gcgaatgact tgtcggtgag cgtgaagagg
     5461 ttgcgcgtgg gagagtgggc gacgcccagg atgatgtaga gccgcggggg acggtc
//
LOCUS       DAOPCO010000266         5514 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12670_length_5514_cov_4.709104, whole genome shotgun sequence.
ACCESSION   DAOPCO010000266 DAOPCO010000000
VERSION     DAOPCO010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12670_length_5514_cov_4.709104"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="PLS90_14385"
     CDS             complement(<1..1970)
                     /locus_tag="PLS90_14385"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPK03632.1"
                     /translation="MADRLASQGFDMVVLHGASGLGLDGDRPNILATKPLSAALKRNG
                     LKRVAYTQAIGFVYYEALQAERPESINWLQRQPNGEHPYYYTAWFWKVPCLNNPDFIA
                     YLKELFRFLMRELDLDGLFLDNFGYHSYSCYCDHCKSRFRKYLYSRYPTAEARYERFE
                     MTNLDYIDPPPFHHLSGCSFDNNGVPEETKMIEPVSQEWIRFRCERAGEIAREFNDCI
                     KECNPDAILYLNYMYGGAPGMNNSVFHGLWPEFIYPNADLVLAEVAGPPSINDAGVLH
                     SRIPMMKTAKHFGIPMSTCSFHRELKDFRRIYLAEGMAFNTAPVDLVGDVERDDPPDW
                     MQAYKSFYREHRTLLGGAATVADCAILHNFESLSYACSYPQESLVLCEQSLIQGGFTF
                     DIIFDQGLNNLDKYPVLFLANVICLSRPVIERIADYVRAGGAVVATEDTSCFNERFIP
                     WRGAWPHRMFKIPLLGELLGIEWPKSEMACCEVGQGRIAVLPAIERPWRDAAGGGPGR
                     YPSPVDMATHSYGPAMPGVLRLPALSAHHDDIGAAMEYALNGRRTLIAEARGPLLTEV
                     THNHQGTFIHLLNWDHTAPIDSIQLSLRWSGDPPASVERISPDAETPSGLLPFQLKEG
                     RIEITLPDLLCYSVVVIKPAPWIARNRREAAMA"
     gene            complement(2135..5038)
                     /locus_tag="PLS90_14390"
     CDS             complement(2135..5038)
                     /locus_tag="PLS90_14390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK03633.1"
                     /translation="MGGRLSSPTVRARYRQPNHYYPVVARMVATPPSAAPTAWGEKPR
                     PATQDFDGDGNLETIRHINAADSVKVQVVSAPDGAVLWEHEISMENRPGLHLIDPIIA
                     DVVPELNGDEAIFFGNWIDVPRKEAMGIENEDGFFGYICCVSYADGVGRMVWENEQMH
                     ITYSPGVIIDDIDEDGIQDIVVSVHFRMFAINGQTGETTHDVFFSHGRNYALVQTFEG
                     EGDVLKRVLISGDFYQETTVVEMYRDADGAVKGRAAWTMLFEDTVGLKNANSYSTMQM
                     IQDLDGDGKQQILLNFFNKAGHRMDSVGGFDNPLGDIDGDGICDGVSVIGEEPYYLEG
                     DGRWHALVLDPNTGRVQQEVPDQFFMGAADLNRSGRPELFMLATHGHTRPGYGRLSLW
                     SDENGRLTERWSAPGARGRIVKTNPGHRPNFAANIVNGGIPIAADLDGDGAFDFLILS
                     DQDGDWNAEHLECFTVSPDPDKLDVTSKWSFDIPAGALQVLAVEPLGQDPGGVTSLVL
                     QDRRTGLRHTLDAATGQEAAEPVEQVEPGGAPWNPKVVAGDFDDDDRLELIACESTYE
                     TVVLEYRPGDPEPFAEQARFPGHGLMAENPLVSDLDDDGRDEVIVARPNEKGEMEIAA
                     YTIDGELLWSTPAAEITFSRTGDRSPGGLTGTPVAADFTGDGVKDVYFSAFREGMGHL
                     EGFAFSGADGSALWHARGIPGHDTYTMIGSRNLAASMDADHDGRQDLVLCSIFIPQLV
                     EGATGNVLTPLKQAHEMFSIDQMSSVYFSPIVADVDGDGRRDIYWFNGRYHVLTDDNL
                     NKKWDFINHSASGAVNLDSLGRMRAPDGPMDIVFIGTVDPPRFSGNVVFNEVRNERLI
                     CADGADGSQRWTWRPDNDEHFNSIPTLVDLDGDRLHEAVVLVGGELYAVKAPREENGE
                     AQVLWEYPLPDYQSAPANIMAVDADGDGNPELLVAGMLGSNGMLYLIDGGETP"
BASE COUNT          994 a         1612 c         1837 g         1071 t
ORIGIN      
        1 gccattgccg cctctcggcg gttcctcgcg atccatgggg ccggtttgat gacgacgacg
       61 ctgtagcaca ggagatcggg gagcgtgatc tcgattcgac cttccttcag ttggaacggg
      121 aggaggcccg agggggtctc cgcgtcgggg gagatgcgtt cgaccgatgc ggggggatcg
      181 cccgaccagc gcagcgacag ttggatggaa tcgatcggcg ccgtgtgatc ccagttgagc
      241 aggtgaatga aggttccctg gtgattatgg gtgacctcgg tcagcagggg gccgcgcgcc
      301 tcggcgatca gtgtcctgcg accgttgagc gcgtactcca tggcggcgcc aatgtcgtcg
      361 tgatgggccg acagcgcggg cagccgcagc acgcccggca tcgccgggcc gtaggagtgg
      421 gtggccatgt caacggggct gggatagcgc cccgggccgc cacccgccgc atcgcgccag
      481 gggcgttcga tggctgggag gacggcgatg cgtccctggc ccacttcaca gcaggccatc
      541 tcggatttgg gccactcgat gccgagcagt tcgcctagca gcgggatctt gaacatccgg
      601 tgaggccatg cgccgcgcca ggggatgaag cgctcgttga agcaggaggt gtcctcggtg
      661 gcgaccacgg cgccgcccgc gcgaacgtaa tcggcgatcc gctcgatcac gggacggctc
      721 aggcagatca cattggcgag gaacaggacc gggtacttgt cgaggttgtt caggccctga
      781 tcaaaaataa tgtcaaaggt gaaaccgccc tggatcaatg actgttcgca gaggaccagg
      841 ctttcctgcg gataggagca ggcgtaggac agcgactcga agttgtgcag gatcgcgcaa
      901 tcggcgaccg tcgcggcgcc tcccagaaga gtccggtgct cacggtagaa gcttttgtaa
      961 gcctgcatcc agtccggcgg gtcgtcgcgt tcgacgtcgc ccacgagatc gacgggggcc
     1021 gtgttgaagg ccatgccctc ggccaggtaa atccggcgga aatccttcag ctcgcggtga
     1081 aaggagcagg tcgacatcgg gatcccgaaa tgtttggcgg tcttcatcat cggaatgcgg
     1141 ctgtgcagca cgccagcgtc gttgatggag ggagggccgg ccacctcggc caggacaagg
     1201 tccgcgttcg ggtagataaa ctccggccac aggccatgga agacggagtt gttcatgccg
     1261 ggcgcgccgc cgtacatgta gttcagatag aggatcgcgt cgggattgca ttccttaatg
     1321 caatcattga actcgcgcgc gatttcgccg gcccgctcgc agcggaagcg gatccattcc
     1381 tgcgagaccg gctcgatcat cttggtctcc tcggggacgc cgttgttatc gaacgagcat
     1441 cccgagaggt gatgaaacgg cggcgggtcg atatagtcca ggttggtcat ttcaaagcgt
     1501 tcgtagcgcg cttccgcggt cggatagcgg ctgtagagat acttccggaa ccggcttttg
     1561 caatgatcgc agtagcagga gtaggagtga tagccgaaat tatcgagaaa caggccgtcc
     1621 agatcgagct cgcgcatcag gaagcggaac agttccttga gataggcgat aaagtcggga
     1681 ttgttcaggc agggaacttt ccagaaccag gcggtgtaat agtacgggtg ttcgccgttg
     1741 ggctgccgct ggagccagtt gatcgactcg ggtcgttccg cctggagggc ttcgtagtac
     1801 acgaagccga tggcttgcgt gtaggcgacg cgcttgaggc cgttgcgttt gagcgcggcg
     1861 gagagcggtt tcgtggcgag gatgttgggg cgatccccgt cgaggcccaa cccgctggcg
     1921 ccgtggagca cgaccatgtc gaatccctgg ctcgcgagcc gatcggccat cgatggctcc
     1981 aggccgcgcc ggatgttctc cagcgtctcg acggtccgat ttccccagcg gatggggatc
     2041 ccctcggccc gttggaccga ggtgattccg caaaggatcc atgccggaac ggccgtggac
     2101 cggttgtttg tgattggcct tgttttcagc atcgtcatgg tgtttcgccg ccgtcaatca
     2161 gatagagcat gccgttggag ccgagcatcc ccgcgaccag caattccgga tttccatcgc
     2221 cgtccgcatc gaccgccatg atgttcgcgg gggccgattg atagtcgggc aaggggtatt
     2281 cccagaggac ctgtgcctcg ccgttttctt cgcgcggcgc cttgacggcg tagagctcgc
     2341 cccccacgag caccacggct tcgtgcaggc ggtcgccatc cagatcaacc agggtgggaa
     2401 tcgaattgaa gtgctcgtcg ttgtccggcc gccaggtcca gcgctggctg ccgtcggcgc
     2461 cgtccgcgca gatcaggcgt tcgttccgaa cttcgttgaa gacgacattg ccgctgaatc
     2521 tggggggatc caccgtgccg ataaagacga tgtccatcgg gccgtcgggc gcgcgcatcc
     2581 ggccaaggga atcgaggttg accgcgcccg acgccgaatg attaatgaaa tcccatttct
     2641 tgttcaggtt atcgtcggtg agcacatggt atcggccatt aaaccagtag atgtcgcggc
     2701 ggccgtcgcc gtcgacgtcg gcgacaatcg gggagaaata gacggaggac atctgatcga
     2761 tggagaacat ctcgtgggcc tgtttcaagg gggtcaagac gttgccggtg gcgccttcga
     2821 cgagctgcgg gatgaagatg ctgcacagga cgagatcctg ccgaccgtca tgatcggcgt
     2881 ccatactcgc cgccagattg cgcgacccga tcatggtgta ggtgtcatga ccggggatgc
     2941 cgcgcgcgtg ccagagcgcg gagccatcgg cgccggagaa ggcaaagccc tcgaggtggc
     3001 ccatgccttc gcgaaacgcc gagaaataga cgtccttgac gccgtcgccg gtgaaatcgg
     3061 cggcgaccgg cgtgccggtg agcccgccgg ggctgcggtc gccggtgcgg ctgaacgtga
     3121 tttcggccgc gggggttgac cagaggagct cgccgtcaat ggtataggcc gcgatttcca
     3181 tttctccctt ttcgttcggg cgggccacga tgacttcatc gcgtccatcg tcatcgagat
     3241 cgctgacgag cggattctcg gccatcagcc cgtggccggg gaagcgggcc tgttcggcga
     3301 acggctcggg gtcgccgggc cggtattcca gcacgacggt ttcgtaggtg ctttcacaag
     3361 cgatgagttc cagcctgtcg tcgtcgtcga aatcaccggc gacgaccttg ggattccagg
     3421 gcgcgccgcc cggttcgacc tgctcgaccg gctcggccgc ttcctggccg gtggcggcgt
     3481 cgagcgtatg gcggagtccg gtccggcgat cctgcaggac aaggctcgtg acgccgcccg
     3541 ggtcctgacc caggggctcg accgccagaa cttgcagggc gcccgcgggg atatcgaacg
     3601 accatttgct cgtgacgtcg agtttatccg gatccgggct gacggtgaag cattcgaggt
     3661 gttcggcatt ccagtcgccg tcctgatcgc tgaggatgag aaaatcgaag gccccatcgc
     3721 catccaggtc ggcggcgatc ggaattccgc cgttgacgat attggcggcg aaattgggcc
     3781 gatggccggg gttggtcttg acgatgcgcc cgcgcgcgcc gggtgcggac cagcgttcgg
     3841 tcagcctgcc gttttcatcg ctccacagac tcaggcgccc gtaaccaggc cgggtgtggc
     3901 cgtgggtggc gagcatgaaa agctcgggcc ggccgctgcg attgaggtcc gcggcgccca
     3961 tgaagaattg atccggcacc tcctgttgca cccggccggt gttggggtca agcaccaggg
     4021 cgtgccagcg gccgtcgccc tcgagatagt acggctcctc gccaatgacg ctgacgccgt
     4081 cgcagatgcc gtcgccgtcg atatcgccca gcggattgtc gaacccaccg acggaatcca
     4141 tccgatggcc ggctttgttg aagaaattca acaggatctg ctgttttcca tcgccgtcca
     4201 gatcctgaat catctgcatc gtcgagtagc tgttggcgtt tttcaggccg accgtgtctt
     4261 cgaagagcat ggtccaggcg gcgcggccct tcaccgcgcc atccgcatcg cggtacattt
     4321 cgaccacggt cgtttcctga tagaaatcgc cggaaatgag cacgcgcttg agcacgtccc
     4381 cttcgccctc gaacgtctgc accagggcgt agttgcgacc gtggctgaag aagacgtcat
     4441 gcgttgtttc gccggtctgg ccgttgatgg cgaacatgcg aaaatgcacc gaaacgacga
     4501 tatcctggat gccgtcttca tcgatgtcat cgatgatgac gccgggactg taggtgatgt
     4561 gcatctgctc attttcccac accatgcgtc cgacgccgtc ggcataggag acacaacaga
     4621 tgtagccaaa gaagccgtcc tcgttctcaa tgcccatggc ttccttgcgt ggaacatcga
     4681 tccagttgcc gaagaagatc gcctcgtcac cgttcagttc gggaacgacg tcggcgatga
     4741 tcggatcgat caggtgcagg ccgggccggt tttccattga aatctcgtgc tcccacaaca
     4801 ccgcgccgtc gggggcggag acaacctgga ccttgaccga atcagcggca ttgatgtggc
     4861 ggatcgtctc cagattgccg tcgccgtcga agtcctgggt ggcgggacgg ggtttttcgc
     4921 cccaggcggt cggcgccgcc gacggcggcg tcgccaccat tcttgcgacg acggggtaat
     4981 aatgattcgg ctggcggtag cgcgcgcgca cggttggtga gctgagtcgg ccgcccaaca
     5041 gatgggatgc tccggtgtta tcaaagtttc taacggccgg ccagttgccc ggttccgatt
     5101 cagcgggctg agtcgccgcc acggccagtg cgccgctctg ggcgtcgtcg tgggttcccg
     5161 gggcctccgc gcgggcgccc gggttaccga gcgaacatgc tgcgatcaaa caaagagcgg
     5221 ccaaccgccg ccgttgaagt gtcatcagac ctcatcctta acagattttt caatgggggt
     5281 tggatttcgg gcgggggtcc tggtccccga gatgaagggg accaggcact tttccaaacg
     5341 cgcgacccga aggataatgg ttattaatag gccaaccagt gccgtgaacg gttcgggggc
     5401 ggcgggtagg agccgatatc ccggccatcg ctgcccgcct tgatcagttc aggcgcctgg
     5461 accttcagat tatccggaaa agcgacataa ttgggattga ccgcctgatc atgg
//
LOCUS       DAOPCO010000267         5482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12751_length_5482_cov_3.555924, whole genome shotgun sequence.
ACCESSION   DAOPCO010000267 DAOPCO010000000
VERSION     DAOPCO010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12751_length_5482_cov_3.555924"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PLS90_14395"
     CDS             <1..682
                     /locus_tag="PLS90_14395"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPK03634.1"
                     /translation="FAELKTQGELTRRAWQFDVQVMNEGPGHVPMHMIKENMDKQLEW
                     CGEAPFYTLGPLTTDIAPGFDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKNDVR
                     EGIIAYKIAAHAADLAKGHPGAQAHDDAISKARFEFRWQDQFNLALDPDRAREFHDET
                     LPAEGAKLAHFCSMCGPKFCSMEITQQVRDYARANGYTSDDEALVRGMQEKSREFEQS
                     GAELYQPR"
     gene            complement(833..2023)
                     /locus_tag="PLS90_14400"
     CDS             complement(833..2023)
                     /locus_tag="PLS90_14400"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPK03635.1"
                     /translation="MADPLKEERTAAAWDGPVPMIDLKAQYATLASELDAAIARVVQS
                     QMFILGPEVDAFEREIERYLGVKHAIGCASGSDAILLALMALGYGPGDEVIMTPYTFF
                     ATAGSTYRLGIRPVFCDIDPVTYNLDPTKIEEKLTRRTRAIMPVHLFGQCADLDAIGR
                     IARGRELAVVEDAAQIIGATYNGRHAGTLGDAGCFSFFPTKNLGAWGDAGMVTTNDDE
                     LAARVRALRVHGGLKTYHHELVGMNSRLDGFQGAVLRAKLPHLSGWNAQRGANAARYR
                     ELFAQAGVLQSEGDQPERGKVIVPAVVRESHIFHQYVMRVHATQRDGLLRHLQSRQIG
                     CAIYYPVPLHLQPCFAELGYRAGDLPEAERASRETLALPIYPELPAAAQERVVEVVAE
                     FLRG"
     gene            complement(2027..2617)
                     /locus_tag="PLS90_14405"
     CDS             complement(2027..2617)
                     /locus_tag="PLS90_14405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPK03636.1"
                     /translation="MSYSVHPTAVIDEPVQIGAGTRIWHFCHVMAGAVIGAECNLGQN
                     VFVAGSVRIGDRCKIQNNVSLYDGVILEDDVFCGPSAVFTNVKHPRSAVSRRGEYEVT
                     RVGRGATLGANCTVVCGVTIGAHAFIGAGAVVTRDVPPYAMMAGVPARRIGWAGPHGE
                     RLVAAEEQPAVFVCPATGRRFVQIDEWTLNPLETES"
     assembly_gap    2912..3011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3240..3986)
                     /locus_tag="PLS90_14410"
     CDS             complement(3240..3986)
                     /locus_tag="PLS90_14410"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HPK03637.1"
                     /translation="MTIRRGMALATAVLVLMAASSVQAITLDLTTAGSSGTINGALFY
                     QADPQPTGTGVINSFLRIQMKGTEQGYNTDGKLEFDQKAGNFTRSLLLSDVPVVTING
                     VQYREFGLDINEKKKGPDSLLSLDRLQFWLGAEPDLDDYSQGLGDLVWDMDAAGDSYV
                     KMDYALNHGSGSGDIFVYVPNAVFTSTNEYVYLFSRFGDHFSSDAGFEEWWVREGKGT
                     PPPVIPEPATLSLIGLGLGSLGLARRKRRA"
     gene            complement(4444..5466)
                     /locus_tag="PLS90_14415"
     CDS             complement(4444..5466)
                     /locus_tag="PLS90_14415"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPK03638.1"
                     /translation="MVRLAVMGATGAVGREMLRTLEQRNFRYSSIKLLASERSAGTKL
                     QFNGAMHPVELLTKDAFGGVDIVLSSAGASVSREFAPAAVAAGAVVIDNTSAFRMDKA
                     VPLVVPEVNAHAAQHHQGIIANPNCSTIQLVVALKPLHEAARIKRVVVSTYQATSGAG
                     NAAMDEMEEQTIAHLNGDELPAPRKFAHTIAFNCLPQIDVFLESGYTKEEMKMVNETQ
                     KIMEDDSIQVSPTCVRVPVYRGHSESVNIETEQPLAPEEARDLLRRAPGVVVVDDPAN
                     GAYPMPLYADGLDPVFVGRIRKDETVANGLNLWIVSDNLRKGAALNAVQIAELLIEKG
                     WVKQAG"
BASE COUNT          908 a         2137 c         1370 g          967 t
ORIGIN      
        1 gttcgccgaa cttaaaaccc agggcgaact gacccgccgc gcctggcaat tcgacgtcca
       61 ggtgatgaac gagggccccg gccacgtgcc gatgcacatg atcaaggaga acatggataa
      121 gcagctcgag tggtgcggcg aagccccgtt ctacaccctc ggcccgctca cgaccgacat
      181 cgcccccggc ttcgaccaca tcacctccgc catcggcgcc gcgatgatcg gctggtacgg
      241 caccgccatg ctgtgctacg tgacccccaa ggaacacctc ggcctgccca acaagaacga
      301 cgtgcgcgag ggcatcatcg cctacaagat cgcggcccac gccgccgacc tcgccaaggg
      361 ccaccccggc gcccaggcgc acgacgatgc gatcagcaag gcccgcttcg agttccgctg
      421 gcaggaccag ttcaatctgg cgctcgaccc cgaccgcgcg cgcgagttcc acgacgaaac
      481 gctcccggcc gaaggcgcca agctcgccca tttctgctcg atgtgcggcc ccaaattctg
      541 ttcgatggaa atcacccagc aggtgcgcga ctacgcccgc gcgaacggct acaccagcga
      601 cgacgaggcc ctcgtccgcg gcatgcagga aaaaagccgc gaattcgagc aatcaggcgc
      661 cgaactctac caaccccgct agtccgatcg gtatcggtat cgcgctcgcg attggtatcg
      721 gtatcgcaat cggtctcgcg atcgctccca aacgccttcc ccaaaactcg gctctcgcct
      781 cccgtccctc gtcctcggct ctcgaatctc ttctctcccc ctctccctcc ccctaccccc
      841 tcaaaaactc cgccaccacc tccaccaccc gctcctgggc cgcagcgggc agctcgggat
      901 aaatcggcag cgccagcgtc tcgcggctcg ctcgttccgc ctccggcagg tcgcccgcac
      961 gataccccag ttccgcgaaa cacggctgca ggtgcagcgg caccggatag tagatcgcgc
     1021 agccgatctg ccggctctgc agatgccgca gcaacccgtc gcgctgcgtc gcatgcacgc
     1081 gcatcacata ctggtggaaa atgtggctct cgcgcacgac cgccggcacg atcactttcc
     1141 cccgttccgg ctgatcccct tcgctctgca acacgcccgc ctgcgcgaac agctcccggt
     1201 atcgcgcggc gttcgccccc cgctgcgcgt tccagccgct caagtgcggc agcttcgccc
     1261 gcaacaccgc cccctgaaaa ccgtccagcc gcgagttcat cccgaccagt tcgtgatgat
     1321 atgtcttgag cccgccatgc acgcgcagcg cccgcacccg cgccgccagt tcgtcgtcgt
     1381 tcgtcgtcac catcccggcg tcgccccagg cgcccaggtt cttcgtcgga aaaaagctga
     1441 agcagcccgc gtcgcccagc gtgcccgcgt gccggccgtt gtacgtcgcg ccgatgatct
     1501 gcgccgcgtc ctccaccacc gccagttccc gcccccgcgc gatccggccg atcgcgtcca
     1561 gatcggcgca ctggccgaac agatgcaccg gcatgatcgc ccgggtccgc cgcgtgagct
     1621 tctcctcgat cttcgtcgga tccagattgt aggtcaccgg gtcgatgtcg cagaagacgg
     1681 gacgaatccc gagccgatac gtcgaacccg ccgtcgcgaa aaacgtgtag ggggtcatga
     1741 tgacctcgtc ccccggccca taccccagcg ccatcagcgc cagcaggatc gcatcggatc
     1801 ccgacgcgca gccgatcgcg tgcttgaccc ccaggtaccg ctcgatctcc cgctcgaacg
     1861 cgtcgacttc cggccccagt atgaacatct gcgattggac aacccgcgct atcgccgcgt
     1921 ccagctcact cgccagcgtc gcatactgcg ccttcaggtc gatcatcggc acggggccgt
     1981 cccacgccgc cgcggtccgc tcttctttca aaggatctgc cacagctcac gattccgttt
     2041 ccaatgggtt cagcgtccat tcatcgatct gcacgaaccg ccgccccgtc gcggggcaga
     2101 cgaacacggc cggttgttcc tcggccgcca ccagccgctc gccgtgcggg ccggcccatc
     2161 cgatccgccg cgccggcacc cccgccatca tcgcgtacgg cggcacgtcg cgcgtgacca
     2221 ctgcccccgc gccgatgaat gcgtgcgccc cgatcgtgac cccgcagacg accgtgcaat
     2281 tggcccccag cgtcgcccct cgccccacgc gcgtcacctc gtattctccc cgccgcgaca
     2341 ccgcgctccg cgggtgcttc acgttggtga acaccgcgga cggtccacag aaaacatcat
     2401 cctccaggat cacgccatcg taaagactca cgttgttctg gatcttgcag cggtcgccga
     2461 tccgcacgct gccggccaca aacacgttct gtcccagatt gcactccgcc ccgattacgg
     2521 cccccgccat cacgtggcag aagtgccaga tgcgggtccc cgccccgatc tgcaccggtt
     2581 catcgatcac ggcggtggga tggacgctgt aactcaactt cggctcccca accggcccgt
     2641 ccctcgttcc tccgggcgct cccgacacac tcaacacggc agcccgcctt cagctcccga
     2701 ccgattcggc cccgcgcgac cggccgcaac agcctagcgc agcccccaac cgattttcaa
     2761 cgagctgaac catccccccc tttcgtgccc cttcgcgcct ttcgcgatcc ccccaaaatc
     2821 cgtccctcct cttccctgtg atcgccccca aaggctttct ctaaaattcg gctctcggct
     2881 ctcgtccctc gtcctcggct ctcgaaatcc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nattcggctc tcggctctcg tccctcgtcc tcggctctcg aaatcccttt
     3061 tctccccctc tttaccaagc accagctcct cgcctctccg ccccgccccc cccttttcgg
     3121 ccccttcgcg cctttcgcga tccccttctg tccttcatgt cccttacgtc cctaccaaaa
     3181 tgcaagcggc ccgaccggtc catcaccgat cgggccgcgt gacctgtcgt ggaacggatt
     3241 tacgcccggc gcttgcgccg cgccaacccc agactgccca ggcccagccc gataaggctc
     3301 agcgtggccg gctcggggat caccggcggc ggggtcccct tgccctcgcg cacccaccac
     3361 tcctcaaagc ccgcgtccga cgagaagtga tcgccgaacc gcgagaacag atacacatac
     3421 tcattcgtgc tcgtgaacac cgcattcggc acgtagacga aaatatcgcc cgacccgctg
     3481 ccgtggttca gcgcgtaatc catcttcaca tagctgtcgc ccgcggcgtc catatcccac
     3541 accaggtcgc ccagtccctg actgtaatcg tccaggtccg gctccgcccc cagccagaac
     3601 tgcagccggt ccagcgacag caggctgtcg ggacccttct tcttctcgtt gatgtccagg
     3661 ccgaattcac gatactggac cccgttgatc gtcaccaccg gcacgtccga cagcagcagc
     3721 gaccgcgtga aattgcccgc cttctgatcg aactccagct tgccgtcggt gttgtagccc
     3781 tgttccgtcc ccttcatctg gatccgcagg aacgagttga tcacccccgt ccccgtcggt
     3841 tgcggatccg cctgataaaa cagcgcccca ttgatcgtgc ccgaggaccc cgccgtcgtc
     3901 agatccaatg tgatcgcctg caccgatgac gccgccatca gcaccaacac cgctgtcgcc
     3961 agcgccatcc cgcgcctgat cgtcatgatc gcactccctt tactccgata ttgatcgggg
     4021 gataatttgc gcccagccgc tggcggcctc cctgccccag caccaagccg gcgatcccct
     4081 ctgcatggcc ttgccgtccg tggacgggca attctcaatg cagccatcgt aaatcaaact
     4141 tcatgccgct tggccggggc aaatccacta cttcccccga tccaatcaaa agccttaaga
     4201 atcaatgttt tgtgttgtct tcaagactgc cccggccgcc cccatgaggc ccggaccgcg
     4261 aaggagatga aatgcatttc acacccagtg tgcagaccac cgtccaatct caaatcggct
     4321 ctcgcctctc gtcccctcgt cctcgaaacg cactccgccc ccccgactgc cctttcgtgg
     4381 ccattcgtgc ctttcgtgat ccctgatgcg tctctcgccc tcgtccccgc taaccgcccc
     4441 ccctcagccc gcctgcttca cccagccttt ctcaatcagc agctcggcga tctgcaccgc
     4501 gttgagcgcc gcccccttcc gcagattgtc gctcacgatc cacaggttca gcccgttggc
     4561 caccgtctca tccttccgga tccgcccgac aaacaccggg tccagcccgt cggcatacag
     4621 cggcatcgga tacgccccgt tggccgggtc gtccaccacc accacccccg gcgcccgccg
     4681 cagcaggtcg cgcgcttcct ccggcgccag cggctgctcc gtctcgatgt tcaccgattc
     4741 gctgtgcccc cgatacaccg gcacgcgcac gcacgtcggc gacacttgga tcgaatcgtc
     4801 ctccatgatc ttctgtgtct cgttcaccat cttcatctct tccttggtgt agccgctctc
     4861 caggaagacg tcgatctgcg gcaggcagtt gaacgcgatc gtgtgcgcga atttcctcgg
     4921 agcgggcagt tcatcgccgt tcaaatgcgc gatcgtctgc tcctccatct cgtccatcgc
     4981 ggcattgccg gcccccgatg tcgcctgata cgtcgagacc accacgcgct tgatccgcgc
     5041 cgcttcgtgc agcggcttga gcgccaccac cagctggatc gtcgaacagt tcgggttggc
     5101 gatgatcccc tggtgatgct gcgccgcgtg cgcgttcacc tccggaacga ccagcggcac
     5161 cgccttgtcc atccggaagg ccgacgtgtt gtcgatcacg accgcccccg ccgccacggc
     5221 cgccggcgca aactcgcgcg acaccgacgc ccccgccgac gacagcacga tatccacccc
     5281 cccgaaggcg tccttcgtca gcagctccac cgggtgcatc gccccgttga actgcaattt
     5341 cgtccccgcc gaccgctccg acgccagcag cttgatcgaa ctgtagcgga aattgcgctg
     5401 ctccagcgtg cgcagcattt cgcggccgac cgcgcccgtg gcgcccatga cggcgagacg
     5461 aaccatgggt cttctccttg ct
//
LOCUS       DAOPCO010000268         5477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12766_length_5477_cov_9.088344, whole genome shotgun sequence.
ACCESSION   DAOPCO010000268 DAOPCO010000000
VERSION     DAOPCO010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12766_length_5477_cov_9.088344"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLS90_14420"
     CDS             complement(<1..289)
                     /locus_tag="PLS90_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03639.1"
                     /translation="MKRIALICWLSLGLAPMIPAANTTWGPAEIDVEITTGTLSVEWT
                     GFERANRIMSPLKLWKIDWSSNPDGDVTFETDQIQGRLAWLVTNPDDGATSP"
     gene            complement(286..504)
                     /locus_tag="PLS90_14425"
     CDS             complement(286..504)
                     /locus_tag="PLS90_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03640.1"
                     /translation="MLKTVTQPSRIYAEGREFDLHAGWAGYLIKSGMAEAVNVDAPEP
                     EPEKPAPEVKPAAKPIFRDRPPTRGKKR"
     gene            complement(525..1091)
                     /locus_tag="PLS90_14430"
     CDS             complement(525..1091)
                     /locus_tag="PLS90_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03641.1"
                     /translation="MKFRFHARPRLIALFAVVIIASAYAQISNVRSRSAGGDTVFYDG
                     ATNIMTIKGGTDGVDFHQTVSFEGAVDRAPLVTISDNAGTTTPVTITAAQNGTHFVLG
                     ENGSNPLTFNLPAAVAGMEFWFTDNDATADADLVVNPDDADTIEDSSAGVSINSTTDE
                     VGPTLGLRAVSATQWVVIGVRGTWAEGS"
     gene            complement(1167..1631)
                     /locus_tag="PLS90_14435"
     CDS             complement(1167..1631)
                     /locus_tag="PLS90_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03642.1"
                     /translation="MKGITIAEQCHVVNILPPVDINGGVSSDVFSLKNWQHATILIQT
                     GVTHAASTVTVEECDDFTPTNHTAIPFAYYEETTAAGDTLSARKQATTSGFATSANDN
                     GFYVIEIDAAQLSEGFPNLIVKFSDPGGATFASAVAILSGGPGQAITDTAIA"
     gene            complement(1668..2963)
                     /locus_tag="PLS90_14440"
     CDS             complement(1668..2963)
                     /locus_tag="PLS90_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPK03643.1"
                     /translation="MLTAHELRQLAARKLAEAKAIQGTGEGGVLTDAEQQQFNALLEE
                     HDRLKAQAESRERLENAEALADTPVNKVPAQQTITNPRDRIEDKPWDSLGEQMLAIRN
                     AYMAGGTVDPRLKNAASGASEGVPSDGGFLVDRPFAEELMTRIHDAGQLASRCRRMSI
                     GANANGLKLRLVDETSRANGSRWGGVQVYWTDEAEEMTASKPKLRTLDLALHKLTGLF
                     YATDELLQDATAMSGYAEMAMTEEFAVKLDDAILNGAGGPTPLGIMNCGALVTVSKEA
                     GQAPDTFVFENANNMRARLWARSRPNSAWFYNQDVEPQLPLMNVSVGTGGTAVYQPAG
                     GAADAPLDRLLGRPMIPIEQAKTLGDLGDIVLLDLSEYLLIEKGGVQSAMSIHVEFKS
                     NQTVFRWVYRVNGAPLWNAALTPMNGTNKVSPFVALEAR"
     gene            complement(2966..3754)
                     /locus_tag="PLS90_14445"
     CDS             complement(2966..3754)
                     /locus_tag="PLS90_14445"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HPK03644.1"
                     /translation="MNRNGKLGFKLKVQNKVASLEIYDAIGDFWGVTPADVSRQLRDA
                     GEVEQINVFLNSPGGDAFDGIAIFNLLKNHPAPVHAFVMGLAASAASIITMAADRIVM
                     ERGAFLMIHNASGLAIGNASVMREMAGLLDKLDGQLAEIYVERSGQDRDQIQQWMDEE
                     TWFTAEEAIEAGLADEAQAAEEGQRAQVRQALGQQPFVYRNVPEEMKNIFVKPAAVIE
                     PAAKANDIPSVDFGSLTERVEKLEILTSTIAAAQKRADAILRRV"
     gene            complement(3744..5045)
                     /locus_tag="PLS90_14450"
     CDS             complement(3744..5045)
                     /locus_tag="PLS90_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HPK03645.1"
                     /translation="MSVRTHIARLAVRLLNLSPTDDYWYQPVAGPTASGVSVSYENAM
                     SYGAVYLAVKIYAESIAQLPLHLFQQKGTRRAIADSAPLNYLVHAEPNAIQTAFEWKE
                     QLGADLQMRGNHYSFKQYARNGRILSLTRLHPDNVTVKLDDAGEKIFEVRQKDGTKRV
                     HRQDEILHVLGMSMDGITGLSPISYARESIGRALAARDYGSRFFANDARPSVYMKSDK
                     VMTDEIRDKYRMEMTQMLAGGNRFKPFIADQGKSLETLSVTPEEAQFIETEGLGIDDI
                     ARFFNLPPHMLKRLERSTNNNIEHQGLEFLTYSLSPWLVRIESALNRSLLTMAEKRAG
                     FYFKFNANALLRGDIKTRFEAYEKGRLGGWLSVNDIRALEDQDPIENGDIYLEPMNYV
                     PAGTPRQAAAPAAAATEPEPTNYRIAFPRNGANGNGAHHES"
BASE COUNT         1030 a         1786 c         1450 g         1211 t
ORIGIN      
        1 tcgggcttgt cgcgccgtcg tccggattcg tcaccagcca cgccagccgc ccctggatct
       61 gatccgtttc gaatgtcaca tcgccgtccg ggttgctcga ccagtcgatt ttccagagtt
      121 tcagcggcga catgatccga ttcgcccgct caaaccccgt ccattcgacc gacagcgtcc
      181 cggtcgtgat ctccacatca atctccgccg gcccccatgt cgtattggcc gcggggatca
      241 ttggcgccag gccaagcgac agccagcaga ttaaggcaat gcgcttcatc gtttcttacc
      301 tcgggttggt gggcgatccc tgaatatggg tttggccgct ggcttgactt ccggcgcggg
      361 cttttccggc tcaggctcgg gcgcatccac gttgaccgcc tcggccatgc ccgacttaat
      421 cagataccca gcccacccgg cgtgtagatc aaactcgcgc ccctcggcat agatgcgaga
      481 gggctgggta acggtcttga gcatgcggac gcgcatcgtc gccattagct gccctcagcc
      541 caagtcccgc gaacgccgat gacgacccac tgcgttgcgc tcacggcgcg cagtcccagt
      601 gtcggcccga cttcgtcggt cgtgctgtta atcgacacgc ccgcgctcga atcttcgatg
      661 gtgtcggcgt cgtcagggtt gaccaccaag tccgcatccg ccgtcgcgtc attgtcggtg
      721 aaccagaact ccatccccgc cacggccgcc ggcaggttga acgtcaacgg gtttgacccg
      781 ttttcgccca acacgaaatg cgtcccgttc tgtgcggcgg tgatggttac gggcgttgtg
      841 gtcccggcat tgtcgctgat tgtcaccagc ggagcgcgat caaccgcgcc ctcaaagctc
      901 acggtctgat ggaaatcaac cccatcggtg ccgcccttga tcgtcatgat gttggtggcg
      961 ccgtcgtaga aaaccgtatc gccgccggcg gaccggctgc gcacgttgga aatctgcgcg
     1021 taggccgatg cgataatcac cacggcgaac agcgcgatca gccgcgggcg cgcgtgaaac
     1081 ctgaatttca ttgtctttat tccttgtatg gaagtgagcc ggacggcggg ggagtgacca
     1141 ccgcccggct tgagtcggcc ttccgcttac gcgatggccg tatcggtgat agcctgtccc
     1201 ggcccgccgc tcaggatcgc cacggccgag gcaaacgtcg ccccgcccgg atcgctaaac
     1261 ttgacgatca gattcgggaa gccttcggaa agctgcgccg cgtcaatttc aatgacataa
     1321 aagccattgt cattggcgga cgtggcgaac cccgaggtcg tcgcctgctt gcgcgcgctc
     1381 aaagtgtcgc ccgcagcggt cgtttcctcg taataggcga acggaatcgc cgtgtgattc
     1441 gtcggcgtga agtcgtcgca ttcctccact gtcacagtcg aggccgcatg cgtcaccccg
     1501 gtctgaatca ggatcgtcgc gtgttgccag ttcttcaggc taaacacgtc gctcgatacc
     1561 ccgccgttga tgtcaaccgg cggcaggata ttcacgacgt ggcactgttc ggcaatggtg
     1621 atgcccttca tgatctgaat ccctccattg gatttgcgcc gcggctatta gcgggcttcg
     1681 agtgccacga acggagaaac tttgttggtt ccgttcatcg gggtgagggc cgcattccac
     1741 agcggcgcgc cattgacgcg gtaaacccag cggaaaaccg tttggttaga cttgaattcc
     1801 acgtggatcg acatcgccga ctgaaccccg cccttttcga tcagcaggta ttcgctcagg
     1861 tccagcagca cgatatcgcc cagatcgccc aacgtcttgg cctgctcgat agggatcatc
     1921 ggccgcccca gcaggcgatc aagcggcgcg tcggccgccc cgcctgcggg ctggtaaacc
     1981 gccgtcccgc ctgtgcccac gctgacattc atcagcggaa gctgtggctc cacatcctgg
     2041 ttgtagaacc aggccgaatt cggacggctg cgagcccaca gccgagcgcg catattgttc
     2101 gcgttctcaa acacgaacgt atcgggcgct tgcccggctt ccttggaaac cgtgaccagc
     2161 gcgccgcaat tcatgatgcc caatggcgtc ggcccgcccg cgccgttcaa aatggcatcg
     2221 tcgagcttca cggcgaattc ctcggtcatc gccatttcgg cataccccga catcgccgtc
     2281 gcgtcctgca gaagctcatc cgtggcgtag aaaagaccgg tcagcttgtg gagggccaga
     2341 tcgagcgtcc ggagtttcgg cttgctcgcc gtcatttcct cggcctcatc ggtccagtaa
     2401 acctggacgc cgccccaccg cgatccgttc gcccggctgg tctcatccac caaccgcagc
     2461 ttgagtccgt tggcgttcgc gccaattgac atgcgacggc aacggctggc caactggccg
     2521 gcgtcgtgaa tgcgcgtcat cagctcttcg gcgaacggac gatcaaccag gaagccgcca
     2581 tcgctcggaa cgccttcact ggcccccgag gccgcgttct tcaaccgagg gtcaactgtc
     2641 ccgcctgcca tataggcatt gcggatggca agcatctgct cgcccagact gtcccaaggc
     2701 ttgtcttcga tccgatcacg cggattggta attgtctgct gggccggaac cttattcacc
     2761 ggcgtatccg ccagcgcctc ggcattttcc agccgctcac ggctctcggc ttgagccttc
     2821 agccgatcat gctcctcaag cagcgcgtta aactgctgct gttcggcgtc cgtgagaacg
     2881 ccgccttcgc ccgtgccctg aatggccttg gcttcggcca gcttgcgcgc cgccaactgc
     2941 cgcaattcgt gcgcggtcaa catggtcaca ccctcctcaa gatggcgtcg gcgcgcttct
     3001 gcgccgctgc gattgtgctg gttaatattt ccagcttctc aacccgctct gtgagcgagc
     3061 caaaatcaac acttgggata tcattagcct ttgccgctgg ctcaattacc gcagcaggct
     3121 taacaaaaat gtttttcatt tcttctggga cattacgata tacaaacggt tgctgcccca
     3181 atgcctgccg cacttgcgca cgctggcctt cttcagccgc ttgcgcctcg tccgcaagcc
     3241 ccgcctcaat cgcctcctcc gcggtaaacc acgtttcctc atccatccat tgctggatct
     3301 ggtcacgatc ctgtcccgac cgctccacgt agatttccgc caactgtccg tcgagcttat
     3361 cgagcaagcc tgccatctct cgcataaccg atgcattgcc aatggccagc ccgctcgcgt
     3421 tatgaatcat caggaatgcg ccgcgctcca tcacgatcct gtcagccgcc atagtgataa
     3481 tgctcgcggc actggcagcc aaccccatca caaatgcatg gacaggcgcg gggtgattct
     3541 tcaacaggtt aaaaattgcg ataccatcaa acgcatcgcc accgggcgaa ttcagaaata
     3601 cgttgatttg ctcaacttcg cctgcatcac gtaactggcg cgacacgtcc gccggcgtca
     3661 ctccccagaa gtcgccaatg gcgtcataga tttcgagaga cgcaaccttg ttttgcacct
     3721 tcagcttgaa tcctagttta ccgttacgat tcatggtgcg ctccgttccc attggcgcca
     3781 ttgcgcggga atgcgatccg gtaattcgtc ggctcgggct ctgtcgcggc cgccgcgggc
     3841 gccgccgctt gccgcggcgt tcccgccggc acataattca tcggctcaag gtaaatatcc
     3901 ccgttctcaa tcgggtcttg gtcttccagc gcccgaatat cattgaccga tagccaacca
     3961 cccagccgcc ccttctcata cgcctcaaac cgcgtcttga tatctccgcg taacaatgcg
     4021 ttggcgttaa atttgaaata gaatccggct cgtttctcag ccattgtcag cagcgagcga
     4081 ttcaatgcgc tttcgatccg caccagccac ggcgaaagcg aataggtcag aaactcaagc
     4141 ccctgatgct caatgttgtt attcgtcgag cgttccagcc gcttgagcat gtggggagga
     4201 aggttgaaaa aacgggcgat atcgtcgata ccaagcccct ctgtttcgat aaattgcgct
     4261 tcctccgggg taacactcag cgtctccagg ctcttgcctt gatcggcgat gaacggcttg
     4321 aagcggtttc cgccggccag catctgcgtc atctccatcc ggtatttgtc gcggatctca
     4381 tccgtcatca ccttgtcgga tttcatgtaa accgatggcc gcgcgtcgtt cgcgaagaac
     4441 cgagagccgt aatcccgcgc cgccagcgcg cgcccgatcg attcccgcgc gtagctgatc
     4501 ggcgacaagc ccgtgatccc gtccatgctc atgcccagca cgtgcaggat ctcatcctgc
     4561 cgatggacgc gctttgtccc gtccttttgc cgcacttcga agatcttttc gcccgcgtcg
     4621 tccagcttca ccgtcacgtt gtccgggtgc agccgcgtca ggctcagaat ccggccgttg
     4681 cgcgcgtatt gcttgaaact gtaatgattc ccccgcattt gcaggtccgc gccgagttgc
     4741 tctttccact cgaacgccgt ctgaatcgcg ttgggctcgg catgcaccag gtaattcagc
     4801 ggcgcgctat cggcaatcgc ccgccgggtg cccttttgct gaaaaagatg gaggggaagc
     4861 tgcgcgatgc tctcggcgta aatctttacg gccaggtaga ccgcgccgta gctcatcgcg
     4921 ttttcgtagg aaacactgac gcccgacgcc gttggacccg ccaccggctg ataccaataa
     4981 tcatctgtcg ggctgagatt cagcagtcgg actgcaaggc gggcgatgtg agttctcaca
     5041 ctcatgcatt gacaatgcgt atacagtcaa tgccaatgca acatttcctt ttttccggaa
     5101 atcctttttt ccggtattcc ggaaatccgg aaatccggaa gtgacaaagc tattgcccga
     5161 ttgcccggat cttgcagcca tccaatagca tagcgatgca cagatcctca atactcactc
     5221 ccgtctcaac cgcacggatt ttgatcgcca aatgtaaatc cttgcgaatt gcaagagtga
     5281 gtttcacgcc atcccggtcc gcatactgtc gcgcctcagc acaagttgtg ttctctgcca
     5341 tatcaattcc cctctctctt tgacctatga aagtggtgta taaacttcaa tctcaccttc
     5401 atcctgatgc ttgatcgcca cagccagcgc aataatcgcc gccaccacgc cgtcaatcct
     5461 ccccgttgac cgcgcct
//
LOCUS       DAOPCO010000269         5460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12804_length_5460_cov_4.597410, whole genome shotgun sequence.
ACCESSION   DAOPCO010000269 DAOPCO010000000
VERSION     DAOPCO010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12804_length_5460_cov_4.597410"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLS90_14455"
     CDS             <1..520
                     /locus_tag="PLS90_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03646.1"
                     /translation="RYEPLEREAVTGLEIKTVNDNWTGFHGWEFRVGERPVAVGKLGR
                     YCFAGSRERHEVRIVPVKRGPYDEPANPSTVIASVLIDAGQAAADGMVYAALAQPVRL
                     EPNTRYMIASYETAGGDPFAADVELTLDPAIEWVGGCYTLSAPDQPGGYWYPVQSIEP
                     GRALNAVSFVMQ"
     gene            531..704
                     /locus_tag="PLS90_14460"
     CDS             531..704
                     /locus_tag="PLS90_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03647.1"
                     /translation="MRRMLYGLALMLAAGVAAGQEGRAELRAIRGDTLSNGLTLELMV
                     PRNDAVGAWWIYR"
     gene            complement(712..1116)
                     /gene="queD"
                     /locus_tag="PLS90_14465"
     CDS             complement(712..1116)
                     /gene="queD"
                     /locus_tag="PLS90_14465"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPK03648.1"
                     /translation="MFHVTKLIDFCYGHRLLKYEGKCRHLHGHNGRVEIRLRGDTLDE
                     RGMLMDFGDVKETMKRWIDDNLDHRMILCRDDPALDYIRQAGELHYAIDENPTAEVIA
                     KLIYDKGREIGLPIVEVVLWETPTSFAAYRRD"
     gene            1641..2738
                     /locus_tag="PLS90_14470"
     CDS             1641..2738
                     /locus_tag="PLS90_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPK03649.1"
                     /translation="MRRLIYLLFCLAGVAPAMAVETDPAVAFQGRLGPAPKYTPSDKD
                     YDSELTKAAGAGLVIDTGNRAEVVTAFNEIFMPAFAYDAEWTGSVNGCIAGTIAADYR
                     QATNDLVNWFRAMAGLPGNVVFDSAIDARCQQAALMMLAAGDLSHSPGTDWACYTAQG
                     AEAAGRSNLALGSAGPDAMAGLMRDPGQNNKDAGHRRWMLYPPQMVMGMGATMEYNAG
                     YFGSCALWVIPTSEFATWGEYPAGIEWYAWPPPGYVPYQAVYPRWSFSQRDADFSGAS
                     VTMRANGEELPFTILSSSTNYGDPALVWELGFDPSFSPPLTDQRVDVTIDYMAPDGGG
                     GTQPAHVEYTVIVIDPASSGESAGRRWEVYR"
     gene            complement(2873..4003)
                     /locus_tag="PLS90_14475"
     CDS             complement(2873..4003)
                     /locus_tag="PLS90_14475"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPK03650.1"
                     /translation="MRVVPVGWRFRPCLSRLAGPRGAARRLALLGLLVGCAAAAQAHI
                     ALTYPTAPQTFVPHQGVEIGWDIYIGHGPGTIRLEYSLDGGQNYTLIVDGIPYSGLPE
                     DRFGSYLWSVPEVETADGRLRVLYTADAGGTFHNGQYGEADPTIAITSTPSLEIVLEN
                     GLDAYDGTRDTTIFAENTNSNGGGEHLFAGTTASPSARRALIGFDLSSIPDGAQIIAA
                     ELGLTVSKTNAGLSAQALHRLTQDWGEGTAKGPGEEGAGAPSQPGDATWVSARRQSAD
                     WATPGGDFVAQPSAVATAGAPGATAHWASAALIADVQHWVDQPSENFGWILIGDESQA
                     GSAKRYYASENATAPEGQRPRLTLRYTLSSGIPVELSSFVLE"
     gene            complement(4141..4431)
                     /locus_tag="PLS90_14480"
     CDS             complement(4141..4431)
                     /locus_tag="PLS90_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03651.1"
                     /translation="MGRTLPTFNMYLDQEIEAWRPFRRALRREDQPAFDRLFALAKRH
                     MAEAAHAARPVPFDSFVMAVLLEQQKEIERLRARLEHPQAEGEFFGTEWPTG"
     gene            complement(4698..4979)
                     /locus_tag="PLS90_14485"
     CDS             complement(4698..4979)
                     /locus_tag="PLS90_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03652.1"
                     /translation="MDRLFLDEDLALNERQYLYERILRRAAALRRRGLPLLITFGGEP
                     ASPWARLLERRTRRAADFNQTLARIEERLARVAPLLGAPPHPALLAGAA"
     gene            complement(4988..5407)
                     /locus_tag="PLS90_14490"
     CDS             complement(4988..5407)
                     /locus_tag="PLS90_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03653.1"
                     /translation="MHARPSTPKRSAVSGNGPLLLPARRAPRILREPGPPPPAASPPD
                     DPRPILSLRTQGPQTGRLLAAFFPLLERGGSVYCLDGANSFDPYRLTRLARKQGRPPE
                     PLLERLFVSRAYTCHQLVGAVETMLPPLAAAAPPRRC"
BASE COUNT          920 a         1506 c         2109 g          925 t
ORIGIN      
        1 gcgctacgag ccgctggagc gcgaggcggt gacggggctg gagatcaaaa cggtcaatga
       61 caactggacc ggattccacg gctgggagtt ccgggtgggc gagcggccgg tcgcggtggg
      121 caagctgggg cgctactgct tcgccggcag ccgcgagcgg cacgaggtgc ggattgtgcc
      181 ggtcaagcgg ggaccgtatg acgagccggc caatccgtcg acggtgatcg cgtcggtgct
      241 gatcgacgcg ggtcaggccg ccgccgatgg gatggtctac gccgcgctgg cgcaaccggt
      301 gcgactggag ccgaataccc gatacatgat cgccagttac gagaccgccg ggggcgatcc
      361 gttcgccgcg gatgtggaat tgacgctgga tcccgcgatc gagtgggtgg gcgggtgcta
      421 tacactctcc gcgccggacc agcccggcgg ctactggtat ccggtgcaaa gcatcgagcc
      481 ggggcgggcg ttgaacgcgg tcagttttgt gatgcaatga ggagccgacg atgagacgga
      541 tgctgtacgg tctggcgctg atgctggcgg cgggcgtggc ggcgggtcag gagggacgcg
      601 cggaactgcg cgcgatccgc ggcgacacgc tgagcaatgg attgacgctg gagctgatgg
      661 tcccgcgcaa cgacgcggtg ggggcgtggt ggatctatcg gtgagcggga atcaatcgcg
      721 ccgataggcg gcgaacgagg tcggggtttc ccagaggacg acctcgacga tcggcaggcc
      781 gatctcgcgg cccttgtcgt agatcagctt ggcgatcacc tcggcggtcg ggttttcatc
      841 gatcgcataa tgcagttcgc cggcctggcg gatgtagtca agcgccggat cgtcgcggca
      901 gaggatcatc cggtggtcga ggttgtcgtc gatccagcgc ttcatcgtct ccttgacgtc
      961 gccgaagtcc atcagcatgc cgcgctcgtc gagcgtgtcg ccgcgcaggc ggatttcgac
     1021 ccggccgtta tggccatgca gatggcggca ttttccctcg tatttgagca gccggtggcc
     1081 gtagcagaaa tcgatgagct tggtgacgtg gaacatgatg gccctttccg gtgttgcggc
     1141 ggagatcggc atcgcgttcc cgcctggcat tccctatttt accccagcgc ggcggagccg
     1201 caaccaccgg aaatggatcg cgggattgga tcgcaaggag gcgggatcgg gatcgagatc
     1261 agaatcgaga tcagaatcga gatcggaatc ggaatgcgtg cgcggtcggt cgatccaaag
     1321 cggtgtcgac gcgttgcgtg gcaacgctct gccaccgcac tccacaaagt cggcgcgacg
     1381 tcggttcatt cggttcatgg ggtggagttt gctggtgcag aggaattgcc gggaaaaggg
     1441 ttgttccagc gggaaggggc gtggcacgat gggggtgcaa tcgaagaatt gcgcaggatg
     1501 atgcgctcga gagccgagga cgaggggcga gagccgaatt ttgagcggca atttgggggc
     1561 gattacgagg acgagggacg agagccgagg gtcgactgtt agagggaggg actcagacct
     1621 attcgttgca ggagttgtga atgagacgtc tgatctattt gttgttctgc cttgccggcg
     1681 tggcgccggc gatggccgtt gagactgatc ccgccgtcgc ctttcaaggg cgactcggcc
     1741 ccgcgcccaa gtacacccca agcgacaagg attacgattc cgagttgacc aaggccgccg
     1801 gggcggggct ggtgatcgac accggcaacc gcgccgaggt ggtgacggcg ttcaacgaga
     1861 ttttcatgcc ggccttcgcg tatgacgccg agtggaccgg ctcggtcaat gggtgcatcg
     1921 ccgggacgat cgcggccgac tatcgccagg cgaccaatga tcttgtgaac tggtttcgcg
     1981 cgatggcggg gctgccgggc aatgtcgtct tcgactccgc gatcgatgca cgttgccagc
     2041 aggcggcgct gatgatgctg gcggcgggcg acctgagcca ctcgccaggg accgactggg
     2101 cgtgctatac ggcgcagggg gcggaagcgg ccggccggtc gaatctggcg ctgggatcgg
     2161 cggggcccga cgcgatggcg ggtctgatgc gcgaccccgg ccagaacaac aaggacgccg
     2221 gtcatcggcg ctggatgctc tatccgccgc agatggtgat ggggatgggc gccacgatgg
     2281 aatacaacgc ggggtatttc ggcagttgcg cgctatgggt gatcccgacg agcgagtttg
     2341 cgacatgggg cgagtacccc gccggcatcg aatggtatgc ctggccgccg ccggggtatg
     2401 ttccgtatca ggcggtctac ccgcgctggt cgttttcgca gcgcgacgcc gacttcagcg
     2461 gcgccagcgt gacgatgcgg gccaacggcg aggagctgcc gttcacgatt ctgtcgtcga
     2521 gcaccaatta tggcgatccg gcgctcgtct gggagctggg attcgatcct tcgttctcgc
     2581 cgccgctgac cgaccagaga gtcgatgtga cgattgatta catggcgccc gacggcggcg
     2641 gcgggaccca gccagcccat gtagaataca cggtgatcgt gatcgacccg gccagctcgg
     2701 gtgaaagcgc gggccgccgc tgggaagtgt atcgttaaat cggtcgcgtg cgtgctccgg
     2761 accgaacggg gtttccgaga gccggggacg aaggacgaga gccgagagcc gaattttgag
     2821 gcggggcttt gggggcgatc aggaggacga ggacgaggga cgacgaggtg agtcactcca
     2881 gtacgaacga ggagagctcg accggaatcc cgctggaaag ggtgtagcgc agggtgaggc
     2941 gggggcgttg gccctcgggg gccgtggcgt tctccgatgc gtaatagcgt ttggcgctgc
     3001 ccgcctgcga ttcgtcgccg atgaggatcc aaccgaagtt ttccgagggc tgatcgaccc
     3061 aatgctggac gtcggcgatc agggcggcgc tggcccagtg ggcggtcgcg cccggcgcgc
     3121 ccgcggtggc gacggcgctg ggctgagcga cgaaatcgcc gccgggcgtt gcccagtcgg
     3181 cgctctgccg gcgggcggag acccaggtgg cgtcgcccgg ctgcgaaggg gcgccggcgc
     3241 cttcctcgcc ggggcccttg gccgttccct cgccccagtc ctgggtgagc cggtgcaggg
     3301 cctgcgcgct gaggccggcg ttggttttgg agacggtcag gcccagctcg gccgcgatga
     3361 tctgggcgcc gtcggggatt gaggagagat cgaagccgat cagcgcgcgg cgggcgctgg
     3421 gcgaagccgt cgtgccggcg aagagatgct cgccgccgcc gttgctgttg gtgttttccg
     3481 cgaagatcgt ggtgtcgcgc gtgccgtcgt aggcgtccag cccgttctcc agcacaatct
     3541 cgagcgaagg ggtcgacgtg atcgcgatcg tcggatcggc ttcgccatac tgcccgttat
     3601 ggaatgtccc gccggcatcg gccgtgtaga ggacgcgcag gcgcccgtcg gcggtttcga
     3661 cctcggggac cgaccagaga taggagccga accggtcctc ggggagcccg gagtagggga
     3721 tgccgtcgac gatcagcgtg tagttctggc cgccgtccag ggagtattcg agccggatgg
     3781 ttccggggcc gtggccgatg tagatatccc agccgatctc gaccccctga tgggggacga
     3841 aggtctgcgg cgccgtgggg taggtgaggg cgatgtgggc ctgcgcggcg gcggcgcagc
     3901 cgaccagcag gccgaggagg gccagccgcc gcgcggcgcc gcgagggccg gcgagacggc
     3961 tgaggcaggg tcgaaaacgc catccgaccg gcacgacgcg catgatcctg ctcctggttc
     4021 cttaaacgca aaatacggga gcgcattgat tcgcaatgga gcaggaatga tatcctgacg
     4081 gccagatcat gtcaattccg gccgtgggga gttgggggga ggggcggggg cgacccgcgt
     4141 tcatccggtt ggccattccg tgccgaagaa ttccccttcg gcttgcgggt gttcgaggcg
     4201 ggcgcgcagg cgttcgattt ccttttgttg ctcgagcagg acggccatga cgaaggaatc
     4261 gaaggggacg ggtcgggcgg catgggcggc ctcggccatg tggcgtttgg ccagggcgaa
     4321 gaggcgatcg aaggcgggct gatcctcgcg gcgcagggcg cggcggaagg ggcgccaggc
     4381 ttcgatttcc tggtcgagat acatgttgaa ggtggggagc gtgcggccca tgggaacctc
     4441 tccgggggaa attcgaaatg cgaagttcga agttcgaaac aaatttcaat gaaaagattc
     4501 ataaaatgcg gaatgcaagg cgatcgggcg gttcggttat tggtatttca gtgagcatcg
     4561 cgggttgccg attccgcagg cttaaggatt ctggcatccc tgccggatgc ggggccgatg
     4621 ccgatgtcga tagcgatcgg aataccgata ccgatacgga ttttgatgct cctgttgcga
     4681 tagtcgaaat tttccgttca tgccgcgcct gcgagcagcg ccgggtgggg cggtgcgccg
     4741 aggagcggcg cgacgcgggc caggcgctcc tcgatgcgcg ccagggtctg gttgaaatca
     4801 gcggcgcggc gggtgcggcg ttcgaggagg cgggcccagg gggaggccgg ttcgccgccg
     4861 aaggtaatca acagcggcag gccgcggcgg cgcaaggccg cggcgcgccg caggatgcgc
     4921 tcgtagaggt actggcgctc gttcagcgcg aggtcctcgt ccagaaacaa gcggtccacg
     4981 ccgaggatca gcaccggcgg gggggggcgg cggccgccag cgggggcagc atggtttcga
     5041 ccgcgccgac cagttgatgg caggtatagg cgcggctgac gaagaggcgt tcgagcaacg
     5101 gttcgggggg gcggccctgt ttgcgcgcca ggcgggtcag gcgatagggg tcgaagctgt
     5161 tggcgccatc gaggcagtaa accgagccgc cgcgttcgag cagggggaag aaggcggcca
     5221 gcaggcgacc ggtttggggg ccctgggtgc gcagcgagag gattgggcgc gggtcgtcgg
     5281 gcggcgacgc ggcggggggg ggcgggccgg gttcgcgcag gatccgcggc gcgcggcggg
     5341 cggggagcaa aaggggtccg ttgccggaaa cggcggagcg tttcggcgtg gaaggccgag
     5401 cgtgcatgat gttttttctc ctggcgaagc cggcgcggga agccgaggaa gggagacagg
//
LOCUS       DAOPCO010000270         5448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12829_length_5448_cov_5.377897, whole genome shotgun sequence.
ACCESSION   DAOPCO010000270 DAOPCO010000000
VERSION     DAOPCO010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12829_length_5448_cov_5.377897"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..1659
                     /locus_tag="PLS90_14495"
     CDS             58..1659
                     /locus_tag="PLS90_14495"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPK03654.1"
                     /translation="MSPLQRWQRYLDTVWHDEELGITLDISRMAFDDGFLGRMADPID
                     RALDGMRELEAGAIANPDEKRMVGHYWLRAPERAPNDELRQAIRAAIDQVQSFAGDIH
                     GANLRGQRDEPFSDFLLIGIGGSALGPQLVADALGTSGDLLRPHFMDNTDPDGIHRVL
                     GELQGRLDRTLVLVISKSGGTPETRNGMVETHAAYAAAGFDFARHAVAVTSTGSRLHQ
                     QAAREKWLAIFPMWDWVGGRTSVTSAVGLLPAALQGVDIDEFLTGARLMDERTRVVDP
                     RNNPAALLALMWHWAGNGRGQRDMVVLPYKDRLMLFSRYLQQLVMESLGKECDLHGNT
                     VHQGLAVYGNKGSTDQHAYVQQLRDGLNNFFVTLIEVLREDAGSESGEPVEVEPGVTS
                     GDFLQGFLLGTREALHEKGRESITITIRSLDARSLGMLIALYERAVGLYASLVMINAY
                     HQPGVEAGKRAAAAILDLQRKLLAAMGEHADYRTAAGWADALGEPERVETIHRLLERL
                     AANPSRRVDRVAGPGPAENTYRLRG"
     gene            2118..3113
                     /locus_tag="PLS90_14500"
     CDS             2118..3113
                     /locus_tag="PLS90_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPK03655.1"
                     /translation="MPTFAETAQAVIENIEKVIIGKRPQILVALAAILARGHILLEDV
                     PGVAKTMLARSIAVTFGLTFKRVQCTPDLLPSDVTGVSIYNQEKREFEFLPGPVFTNI
                     LLADEINRATPRTQSALLEAMEERQISEGGLTRRLPQPFIVMATQNPVEQEGTFPLPE
                     AQLDRFLVRISLGYPDIDQEADMLERLRGGHPLERLKPILNEKTVLQMQQVAAGVFVH
                     AQVRQYLLRIVHGTRDSVKLGLGASPRASIALYRTAQAVAALQGRGYVTPDDVKQLAV
                     PVLAHRLILKPEFRLRKETVQSVVEEVLRGVAAPTNIPAMRPPRQEGPPEILVQG"
     gene            3724..4371
                     /locus_tag="PLS90_14505"
     CDS             3724..4371
                     /locus_tag="PLS90_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03656.1"
                     /translation="MSLTLSLGILADVCDDLEDEASTSAAEDREFLEAYERHLDRINE
                     ILTAAGLPEHDEPDFYPDPEGSDDEGPEWSYTLREGELERLLERIRQAITEGDLTADA
                     DMTSHLLDHSTTDGCYVPLDFPVLLRKGRLTVGSSHQLLRLMEALAQSLDIPAPPAAA
                     PIDYSERGEELQAWLADRLGRPFGNHIDDPCRRLCLALYQGARLSIKYEMLLTLA"
     gene            4574..5101
                     /locus_tag="PLS90_14510"
     CDS             4574..5101
                     /locus_tag="PLS90_14510"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HPK03657.1"
                     /translation="MLTALLFLAAPLPHAQDEPPPLPLDDLSRTTESQQMDSLLRVAK
                     AVPDLKIFLELARGAGLEQTLRIQGPLTVFAPTDAAFRDLPSHELDALRRDPPRCRKF
                     LLQHMVRLRYPSNFIVGEMKLPSLGMTLIMLKKDHAGLKVNDRRAVKVDLVAANGTLH
                     VVDRLLAPLPPADQK"
     gene            complement(5092..>5448)
                     /locus_tag="PLS90_14515"
     CDS             complement(5092..>5448)
                     /locus_tag="PLS90_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03658.1"
                     /translation="SAPAAPIMPAGAANAQTPRPAAPPPAPTPAARRDPSPALMAAAR
                     QTGVQIVRYEEQSPGTAVITLNWSGDVATVGGDFLSEALAANVIRNFDVPALDQGRQL
                     LADGRSVWWSTYRIYF"
BASE COUNT          898 a         2062 c         1629 g          859 t
ORIGIN      
        1 ccttacgtcc cttatgtccc ttccgacacc agaccttcag acgagaggat taaccggatg
       61 agcccgttgc aacgctggca gcgctacctg gataccgtct ggcacgacga ggaactggga
      121 atcacgctcg acatcagccg gatggctttc gacgacggct tcctcggccg catggccgac
      181 ccgatcgacc gcgccctgga cggcatgcgc gagctggagg ccggcgccat cgccaatccc
      241 gacgagaaac ggatggtcgg ccactactgg ctgcgcgcgc cggaacgggc ccccaacgac
      301 gagttgcgcc aggcgattcg cgccgccatc gaccaggtcc aatccttcgc cggcgacatc
      361 cacggcgcca acctgcgcgg ccagcgcgac gaacccttct ccgatttcct cctcatcggc
      421 atcggcggct cggcgctcgg gccgcaactc gttgccgacg ccctgggcac ctcgggcgac
      481 ctgctccgcc cccacttcat ggacaacacc gatcccgacg gcattcaccg cgtcctcggc
      541 gagcttcagg gtcggctcga ccggacgctg gtcctcgtca tctccaaatc gggcggcacg
      601 cccgaaaccc gcaacggcat ggttgaaacc cacgcggctt acgcggccgc cggcttcgac
      661 ttcgcgcgcc atgccgtcgc cgtcaccagc accggcagcc gcctgcatca gcaggccgcc
      721 cgcgaaaaat ggctcgccat tttccccatg tgggactggg tcgggggccg caccagcgtc
      781 acgtccgccg tcggcctgct ccccgccgcg ctgcaagggg tcgacatcga tgagttcctg
      841 accggcgccc gcctgatgga cgaacgcacc cgcgtcgtcg acccgcgcaa caaccccgcc
      901 gccctgctcg ccctgatgtg gcactgggcc ggcaacggcc gcggccagcg cgacatggtc
      961 gtcctgccct acaaggaccg gctgatgctg ttcagccgct acctccagca gctcgtgatg
     1021 gaatcgctcg gcaaggagtg cgacctgcac ggcaacaccg tccaccaggg actggccgtc
     1081 tacggcaaca agggctcgac cgaccagcat gcctatgtgc agcagttgcg cgacgggttg
     1141 aacaatttct tcgtcacgtt gatcgaagtc ctgcgcgaag acgccgggtc ggagtccggc
     1201 gagccggtcg aggtcgagcc gggcgtcacc tccggcgact tcctccaggg attcctcctc
     1261 gggacgcgcg aggcgcttca cgaaaagggc cgcgaatcga tcacgattac gatccgcagc
     1321 ctcgacgccc gctcgctcgg catgctcatc gccctctacg agcgcgccgt cggcctctac
     1381 gcctcgctcg tcatgatcaa tgcctatcat cagccgggcg tcgaggccgg caagcgcgcc
     1441 gccgccgcca tcctcgatct ccagcgcaag ctgctggccg ccatgggcga gcacgccgac
     1501 taccggaccg ccgccggctg ggccgacgcc ctcggcgaac ccgagcgcgt cgagacgatc
     1561 caccgcctgc tggagcgcct ggcggccaat ccctcgcggc gcgtcgatcg cgtggccggg
     1621 cccggtcccg ccgaaaacac ctatcgcctg cgcgggtgag gggcgcgccg cctgcaacca
     1681 ttccgccccc aaccgcgtcc cgttggacgt cgcatcgttc tccagagtgg ccgatcacga
     1741 taggctcatg aaccggcggt tcactcccga gagcgaaagc cggagccgtt atcgaccgat
     1801 tcaatgggct gcgggcttgc cggttcgagc agcccgccag ttccgtcagg gacggcggca
     1861 ccttcctgaa gcgctggatc cggccgatca tcagcccgcc agtcccgcgg ggacggcaga
     1921 agagcacggc gcagaggaac ccgtcgacga cccagcccgc cagtcccgca gggacggcag
     1981 acttcagccc gccacgtcag tggcgggata cgggaaacag gaacgaaaga gtcccgcagg
     2041 gacggcagaa cggctcgaat cactcatcga gaaccgtcgg caacatcccc tcttcaactg
     2101 cttacgaggc tcacggcatg cccacattcg ccgaaaccgc ccaggccgtc atcgagaaca
     2161 tcgagaaggt catcatcggc aaacggccgc agatccttgt cgcgctggcg gcgatcctcg
     2221 cccgcggcca catcctgctc gaggacgtcc cgggcgtcgc caaaaccatg ctcgcccgct
     2281 cgatcgccgt caccttcggc ctcaccttca aacgcgtcca gtgcaccccc gacctgctcc
     2341 cctccgacgt gaccggcgtc tcgatctaca accaggaaaa acgcgaattc gagttcctcc
     2401 ccgggccggt cttcactaac attctgctgg ccgacgaaat caaccgcgcc acgccgcgca
     2461 cccaatccgc tctgctcgag gcgatggagg agcgccagat ctccgagggc ggcctcacgc
     2521 gccggctccc ccagccgttc atcgtcatgg cgacgcagaa ccccgtcgag caggagggga
     2581 ccttcccgct tcccgaggcg cagctcgacc gcttcctcgt ccgcatctcg ctcggctatc
     2641 ccgacatcga tcaggaagcc gacatgctgg agcggctgcg cggcggccac ccgctcgagc
     2701 gtctcaaacc gatcctcaac gagaaaaccg tcctccagat gcagcaggtg gcggccgggg
     2761 tcttcgtcca cgcgcaggtc cgccagtatc tgctgcggat cgtccacggc acgcgcgaca
     2821 gcgtcaagct ggggctcggc gccagcccgc gcgcctccat tgccctctac cggacggcgc
     2881 aggccgtcgc ggccttgcag ggccgcggct acgtcacgcc cgacgacgtc aagcagctgg
     2941 ccgtcccggt cctggcccac cgcctgatcc tcaagccgga gttccgcctg cgcaaggaaa
     3001 ccgtgcaatc ggtggtcgag gaggtgctgc ggggcgtcgc ggcgccgacc aacatccccg
     3061 ccatgcgccc cccgcgccag gaaggccccc ccgagatcct cgtccaaggc tgaatttcat
     3121 cgtccctcgt cctcggcctc cacctgggaa cgccactctc ctgagtggct tggcccacca
     3181 tgtttccgcg ctttgggtgc ccgcgccagc gggctttctt ctggcccctc ggggccctgt
     3241 tatcagcact tgtcattccc caaatcgcaa gctgtttaag gcttccaagt tcaatgcagg
     3301 cggttccgac ttgttgaccg gatatgtccg cgaagcggac gagtggaata aagcgcaggg
     3361 tgacggccgc aggccggaac cctgcgtcgc agcacccaac gcatcaaacc cctgttaggg
     3421 gtggccggag ctggtctgaa tttgggaatg acaagttgtt ggcgccgggc ctcggcccgg
     3481 tgaccctcaa cgacaaattc atcctgactg tcccccgaca ccccacccaa accgatgtcc
     3541 gccccattcc ttcgacctcg gcggttcgcc tggtttcccc cggttgcgcc aacgatcccc
     3601 ttccaaatcg ccgaaccttc cgagataatg ccggtttacg cgacgcgcgc cttcgtcgac
     3661 gacaccccgc cgccggctcg ctgcgcccac cccttccgac cctccgcaac gaaggaaccg
     3721 ttcatgtcgc tgaccctgtc cttgggcatc ctggccgatg tttgcgacga cctggaggac
     3781 gaagcctcga cctccgcggc cgaggatcgc gaattcctcg aggcctacga acggcacctc
     3841 gaccggatca acgagatcct gacggccgcc gggttgcccg agcacgacga gcccgacttc
     3901 taccccgatc ccgaaggctc cgacgacgag gggccggagt ggagctacac gctgcgcgag
     3961 ggcgagctgg aacgcctgct ggagcggatt cgccaggcga tcaccgaagg cgacctgacg
     4021 gccgatgccg acatgacttc gcacctgctc gaccacagca cgaccgacgg ctgctatgtt
     4081 ccccttgatt tcccggtcct gcttcgcaag gggcgcctta cggtgggatc gtcgcaccag
     4141 ctcctgcggc tgatggaggc gctggcccaa tcgctcgaca tccccgcccc gccggccgcg
     4201 gcccccatcg actacagcga acggggcgag gaactgcagg cctggctggc cgatcgcctc
     4261 gggcggccct tcggcaacca tatcgacgac ccctgccgcc gactctgcct ggcgctgtat
     4321 cagggggcgc gcctgtcgat caaatacgag atgctgctga cgctcgcctg atccccggcc
     4381 cggcgccccc gccggcgcct ctcgcggatt ccgctggttt tcccgccgcc gccggactag
     4441 attatcgatc agcgcgcatc ctgcctgcgc gggcgccgcg cgcactccct tcttccgccg
     4501 gaggaatccg tccttgtccc ccgacagcat cccgcggagt tccgttggcc gactcctggc
     4561 acgtccgctc ccgttgctga ccgccctcct gtttctggcc gcgccgctcc cccatgctca
     4621 ggatgagccg ccccccctgc cgttggacga cctgtcgcgc acgaccgaat cgcagcagat
     4681 ggacagcctg ctccgcgtcg cgaaggccgt tcccgatctg aagattttcc tcgaactggc
     4741 ccggggcgcc ggcctggaac aaaccctgcg catccagggc ccgctgaccg tcttcgcgcc
     4801 gaccgacgcc gcttttcgcg acctgccttc ccatgaactg gacgccctgc gccgcgaccc
     4861 gccgcgctgc cgcaagttcc tgctccagca catggtgcgg ctgcgctacc cctcgaactt
     4921 catcgtcggg gaaatgaaac tgccctcgct cggcatgacg ctgatcatgc tgaaaaagga
     4981 ccacgccggc ctgaaggtca atgaccggcg cgcggtgaag gtcgacctgg tcgcggccaa
     5041 cggcacgctt cacgtcgtcg atcgcctgct ggcgcccctc ccgccggccg atcagaagta
     5101 aatccggtag gtgctccacc agaccgagcg cccatcggcc agcagctggc gcccctgatc
     5161 cagggccggc acgtcgaagt tccggatcac attggccgcc agcgcctcgc tgagaaaatc
     5221 ccctccgact gtcgccacgt cccccgacca gttgagcgtg atcaccgccg tgcccgggga
     5281 ttgttcctcg tagcgcacga tttgcacccc cgtctggcgc gcggcggcca tcaacgccgg
     5341 cgacgggtcg cgccgggccg cgggcgtcgg cgcgggcggc ggcgccgcgg ggcgcggcgt
     5401 ctgagcgttg gctgcgccgg ccggcatgat cggcgccgcc ggcgcgct
//
LOCUS       DAOPCO010000271         5419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_12910_length_5419_cov_5.710104, whole genome shotgun sequence.
ACCESSION   DAOPCO010000271 DAOPCO010000000
VERSION     DAOPCO010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12910_length_5419_cov_5.710104"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLS90_14520"
     CDS             <1..411
                     /locus_tag="PLS90_14520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03659.1"
                     /translation="GIADRVHFLGAVSHEDAVALLHAAAVLVLPSHQRSEAFGLCQIE
                     AMLCELPVISTDLPTGVPEVNRNGVTGLVVPPGDVKALRHALNRLLGDAELRKRMGSA
                     GRDRALSEYSDTLMAHRVMEVYAQVLGNKRASYS"
     gene            complement(402..1736)
                     /gene="accC"
                     /locus_tag="PLS90_14525"
     CDS             complement(402..1736)
                     /gene="accC"
                     /locus_tag="PLS90_14525"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPK03660.1"
                     /translation="MFQKILIANRGEIALRIIRACRELGIKTVAVYTEVDRDSLHVRY
                     ADEDVCIGPGTADAYRNAPQIISAAEITDADAIHPGYGFLAENADFAEICEQCRIKFI
                     GPSAKAIRMMGDKAAARKAMMKAGVPVTPGSKDAVSGLEEAIQVAHEIGYPVMLKAAA
                     GGGGRGMRVAHTDVALAGALATAQAEARAAFGSGEVYLEKLVMRPKHVEIQILADSHG
                     RVIHLGERDCSIQRRHQKLVEESPCPTLDDKLRRDMGAAAIKAARAARYEGAGTVEFL
                     LEDRKFYFMEMNTRIQVEHPVTEELTGIDLIKEQIRIASGEKLGYRQKDIEFRGHVIE
                     FRINAEDPDYNFAPNPGKLTTFHVPGGPGVRIDSHAYSEYVIPPHYDSMIAKLIVKGK
                     NRAEAVRRANRALDEFVVEGIRTTIPFFLKILRNERFLSGDYTTAFVEEFYE"
     gene            complement(1795..2262)
                     /gene="accB"
                     /locus_tag="PLS90_14530"
     CDS             complement(1795..2262)
                     /gene="accB"
                     /locus_tag="PLS90_14530"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPK03661.1"
                     /translation="MDLDVIRELIQLMKEHQIAELDWQREGNHIRLKSAAVAGPPAPL
                     PAAQPLALPPATQAEGAPDAGTAGEAPREEGIVITSPIVGMFYRSPSPEKPPFVEIGD
                     AVDENTVLCIIEAMKVMNEIKAEAVGTVQRILVENGQPVEYGQPLFVLTPPGA"
     gene            2487..4133
                     /locus_tag="PLS90_14535"
     CDS             2487..4133
                     /locus_tag="PLS90_14535"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPK03662.1"
                     /translation="MRIALAQINPTVGALDANRRLILDAYRRAADLGADLVVYPELAL
                     TGYPPKDLLDLPCFLRRAMEGLDRLAAEMTGPGAIVGYVHSARDAGETHGKGIYNAAA
                     LIDAGAVRSRHFKSLLPTYDVFDEARYFEPAHEVCVAEFRGRRLAITICEDFWSQTLY
                     ELLNRRIYHRDPLAELAGQGVDLMIHIAASPFTLGKVQVKAAMYRAAARRWGKPLIHV
                     NVVGGNDNLIFDGGSNVWNAEGRVVAQAPQFEEALLLWDSDAPGEGREPDPEHEESAL
                     YGALKLGIHDYVAKCGFERVLLGLSGGIDSALTAVLAADALGPARVTGVAMPSRFSSR
                     HSRDDARRLADNLAIDFHTLAIEPIYQAYLAQLEPLFAGTPFGLAEENLQARTRGNLL
                     MALSNKFGWLLLSTGNKSEMAVGYCTLYGDMSGGLCVLGDVLKTKVFRLSRWLNRRGA
                     RIPESTLTKPPSAELRPNQKDSDSLPEYDILDPIIEAYVERREGPEEIIAAGHDAQLV
                     RRVLRMIDRSEFKRQQAAPLLKVTAKAFDTGRRMPIARGD"
     gene            4344..4604
                     /locus_tag="PLS90_14540"
     CDS             4344..4604
                     /locus_tag="PLS90_14540"
                     /inference="COORDINATES: protein motif:HMM:NF014022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HPK03663.1"
                     /translation="MNSPQIQAELSLYPLEQQDIAKPIYEFVEALERDELKVYIGTLS
                     TVISGEMQRVFDAVRDAYARVARDGRCVLVVKYLNESDKRED"
BASE COUNT          906 a         1940 c         1633 g          940 t
ORIGIN      
        1 ggcatcgccg accgcgttca ctttctgggg gccgtctccc atgaggatgc cgtcgccctg
       61 ctgcacgccg ccgccgtcct ggtccttccc tcccaccagc gttcggaggc cttcggcctg
      121 tgccagatcg aagcgatgct ctgcgagctg cccgtcatct ccaccgacct gccgaccggc
      181 gtgccggagg tcaatcgcaa cggcgtcacc ggtctggtcg tgccgccggg cgatgtcaag
      241 gccctgcgcc acgcgctcaa tcgcctcctc ggcgacgccg agctgcgcaa gcgcatgggc
      301 tcggccggcc gcgatcgcgc cctcagcgaa tacagcgaca cgctcatggc gcaccgcgtc
      361 atggaggtct atgctcaggt gttggggaac aagcgggcga gttactcgta gaactcttcg
      421 acgaacgccg tggtgtaatc gcccgaaagg aagcgttcgt tgcgcaggat cttcaggaag
      481 aacgggatcg tcgtccggat cccttccacc acaaactcat cgagcgcccg gttggcccgc
      541 cgcaccgcct cggcgcggtt cttccccttg acgatcagct tggcgatcat cgagtcgtag
      601 tgcggcggga tcacgtattc gctgtaggcg tgcgaatcga tccggacacc cgggcccccc
      661 ggaacgtgaa acgtcgtcag tttgcccggg ttgggcgcaa aattatagtc cgggtcctcg
      721 gcattgatcc ggaactcgat cacgtggccg cggaactcaa tgtccttctg ccggtagccc
      781 agcttctccc cgctggcgat gcggatctgt tccttgatca ggtcaatccc cgtcagctcc
      841 tccgtcacgg ggtgctccac ctggatccgc gtgttcatct ccatgaagta aaacttgcgg
      901 tcctccagca gaaactcgac ggtcccggcc ccctcgtagc gcgccgcgcg cgccgccttg
      961 atcgcggccg cccccatgtc gcgccgcagc ttgtcgtcca gcgtcgggca cgggctttcc
     1021 tccaccagct tctgatgccg gcgctggatc gagcagtcgc gttcgcccag gtggatcacc
     1081 cggccatggg aatcggccag gatctgaatc tcaacatgct tggggcgcat gacgagcttt
     1141 tccaggtaca cctcgccgct gccgaacgcg gcgcgcgcct cggcctgcgc cgtcgccagc
     1201 gccccggcca gggccacgtc ggtatgcgcg acgcgcatcc cgcggccgcc gccccccgcc
     1261 gccgccttca gcatcaccgg gtagccgatc tcgtgggcca cctggatcgc ttcctccagc
     1321 cccgagaccg cgtccttgct ccccggggtc accggcacgc ccgccttcat catcgccttg
     1381 cgcgccgccg ccttgtctcc catcatccgg atcgccttgg ccgagggccc gatgaacttg
     1441 atccggcact gctcgcagat ctcggcgaaa tcggcgttct ccgccagaaa gccgtagccc
     1501 ggatggatcg cgtcggcgtc ggtgatctcg gccgccgaga tgatctgcgg cgcattgcga
     1561 tacgcgtcgg ccgtccccgg gccgatgcac acgtcctcgt cggcatagcg cacgtgcagc
     1621 gaatcgcggt cgacctccgt gtacacggcg accgtcttga tgcccagctc gcggcacgcg
     1681 cggatgatcc gcagggcaat ctctcctcgg ttggcaatca ggatcttctg gaacatgggc
     1741 ctcgcgactg ctgcggggga tgggagcggc gggcggccgg ccggtccgcg aacctcacgc
     1801 gcccggcggc gtcagcacga acagcggttg accgtactcg accggttggc cgttttcgac
     1861 cagaatccgt tgcaccgtcc ccaccgcctc ggccttgatc tcgttcatca ccttcatcgc
     1921 ctcgatgatg cacaacaccg tgttttcatc gacggcgtcg ccgatctcca cgaacggcgg
     1981 tttctccggc gagggcgacc ggtagaacat cccgacgatc gggctggtga tgacgatccc
     2041 ttcctcgcgc ggcgcctccc cggccgttcc cgcgtccggc gcgccctccg cctgcgtcgc
     2101 ggggggcagc gccagcggtt gcgccgccgg cagcggcgcc ggcgggccgg caaccgcggc
     2161 cgacttcagg cggatatggt ttccctcgcg ctgccagtcc agctcggcaa tctgatgctc
     2221 tttcatcagt tgaatcagct cgcgaatcac atccaggtcc atccgcgcac ctctggggga
     2281 gttggcgccg tcgcgtatgc cggtctgacg cggggtctgt tcgctccgcc cggtccgtca
     2341 tctttccggc cggttggcaa gtcttctata gtcgataccc tctcccttcc tgtcaacgcc
     2401 ttaacccgtt ctttcccaat tcacagcgtc agcccgcctg gcgtaacctg ttgggcgaac
     2461 gaccgacccg aaccgcggag cgggagatgc gcatcgccct ggcccagatc aatccgaccg
     2521 tcggcgccct cgatgccaac cggcgcctga ttctcgacgc ctaccgccgc gccgccgacc
     2581 tcggcgccga cctcgtcgtc tatcccgaac tggcgctgac cggctatccc cccaaggacc
     2641 tgctcgacct gccctgcttc ctgcgccgcg cgatggaggg tctcgaccgg ctggccgccg
     2701 agatgaccgg cccgggcgcc atcgtcggct acgtccattc cgcccgcgac gccggcgaaa
     2761 cccatggcaa ggggatctac aacgccgcgg cgctgatcga tgccggcgcc gtccgctcgc
     2821 gccatttcaa atcgctcctg ccgacctacg atgtcttcga cgaagcccgc tacttcgagc
     2881 ccgcgcacga ggtgtgcgtc gccgagttcc gcggccggcg actcgccatc acgatctgcg
     2941 aggacttctg gagccagacc ctctacgaat tgctcaatcg ccgcatctac caccgcgatc
     3001 cgctcgccga actggccggc cagggcgtcg atctgatgat ccacatcgcc gcctcgccct
     3061 tcactctggg caaggtccag gtcaaggccg cgatgtaccg ggcggcggcc cggcgctggg
     3121 gcaagccgct gatccacgtc aacgtcgtcg gcggcaacga caacctcatc ttcgacggcg
     3181 gcagcaacgt ctggaacgcc gagggccgcg tcgtcgccca agccccccag ttcgaggaag
     3241 ccctcctgct gtgggacagc gacgccccgg gcgaggggcg cgagcccgac ccggaacacg
     3301 aggaatcagc gctctacggc gcgctcaagc tgggaatcca cgactacgtc gccaagtgcg
     3361 gcttcgagcg cgtcctgctc ggtctgtccg gcgggatcga ttccgccctg accgccgtgc
     3421 tggccgccga cgccctcggc cccgcgcgcg tcaccggcgt ggccatgccc tcgcgcttca
     3481 gcagccggca cagccgcgac gatgcccgcc gcctcgccga caacctcgcg atcgacttcc
     3541 acaccctcgc catcgagccg atctatcagg cctatctggc ccagctcgaa ccgctcttcg
     3601 ccggcacgcc cttcggcctg gccgaggaga acctccaggc ccgcactcgc ggcaacctgc
     3661 tgatggcgct cagcaacaag ttcggctggc tcctgctctc caccggcaac aaatccgaga
     3721 tggccgtcgg ctactgcacg ctctacggcg acatgagcgg cggcctgtgc gtcctgggcg
     3781 acgtgctcaa gacgaaggtc ttccgcctgt cgcgctggct caaccggcgc ggggcgcgga
     3841 tccccgagtc gacgctgacc aaacctccct cggccgaact gcgtcccaat cagaaggaca
     3901 gcgattcgct gcccgaatat gacatcctgg atccgatcat cgaggcctat gtcgaacggc
     3961 gcgagggccc cgaggagatc atcgccgccg gccacgacgc ccagttggta cgccgcgtgc
     4021 tccggatgat cgaccgcagc gaattcaagc gccagcaggc cgcgccgctg ctcaaggtga
     4081 ccgccaaggc cttcgacacc ggccggcgca tgcccatcgc gcggggcgat tgatccggct
     4141 cgccgcccgg actgaatcgg gatcgggatc ggagaatcgg agcgaaaaaa tcggagcgaa
     4201 aaccgccccc tggcgggcga gcagggcggt ttcacccggc ccgtcctata atatcggcag
     4261 agctctcgcg ccacgcccag tcccgtcagg gacggcaaaa ccgctcgaat cgatcacgac
     4321 catctcattg gaaggacgat ccgatgaatt ccccgcagat ccaggccgag ctgagcctct
     4381 accccctcga acagcaggat atcgccaaac cgatctacga gtttgtcgaa gcgctcgaac
     4441 gcgatgaact gaaggtttac atcgggacct tgagcactgt catcagtggc gagatgcaac
     4501 gggtcttcga cgccgtccgc gacgcctacg cccgcgtcgc ccgcgacggc cgctgcgtcc
     4561 tcgtggtcaa gtacttgaat gagtccgaca agcgcgagga ctgaagaagg gtcatgccca
     4621 caggctgcgc atgaacgggc ggtttatagc gagggatgaa aatgagaagt cgtgaacgac
     4681 catttcgaat agaggaggcc atccggcctc gctccccggc cagtcccgtc agggatgaaa
     4741 atatgagatc gtgagcgacc gtttcgaatg gccggggcct ccccggcctc gctccccggc
     4801 cagtcccgtc gggatgaaaa atgagatcgt gagcgaccat ttcgaatggc cggggcctcc
     4861 ccggcttcgc cccccggcca gtcccgtcgg gatgagaata tgagatcgtg agcgaccatt
     4921 tcgaatggcc ggggcctcgg cctcgctccc cggccagtcc cgtcagggat gaaaatatga
     4981 gatgtgaacg accatttcga atggccgggg tctctccggc ctcgctccct ggccagtccc
     5041 gtcgggatga aaatatgaga tgtgaacgac catttcgaat ggccggggcc tccccggtct
     5101 cgctccccgg ccagtcccgt cgggatgaaa atatgagatc gtgagcgacc atttcgaatg
     5161 gccggggcct ctccggcctc gctccacggc cagtcccgtc agggacggca gacttcagcc
     5221 cgccacgtca gtggcgggat acgggacgca ggaacgaaag gagtcccgga gggacggcag
     5281 aacccttcga agtcgatctt gaaacgcgta acgtcttttc ggatctcatg tcccttacgt
     5341 cccttatgta ccttatgtcc cttccgacac caaaccttca gacgagagga ataaccggat
     5401 gagcccgttg caacgctgg
//
LOCUS       DAOPCO010000272         5381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13021_length_5381_cov_3.897484, whole genome shotgun sequence.
ACCESSION   DAOPCO010000272 DAOPCO010000000
VERSION     DAOPCO010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13021_length_5381_cov_3.897484"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1308
                     /locus_tag="PLS90_14545"
     CDS             430..1308
                     /locus_tag="PLS90_14545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPK03664.1"
                     /translation="MHTVEVIAHRGASASAPENTLAAFTRAADLGADAIELDCRLTRD
                     GQAAVIHDAALDRVNALGGEVASFPLAELQTIDVGSWFGPDWSDQTIPSLGQALDLAR
                     DRGLGVYIEMKAEAEPAGLIDRLRGDYAGRGRLTRDELARAVLLIEGFQSPTARLVRR
                     VVEEVRARGMEEQVLLQSFSPIACAVALHAAPELPVFLIGESNPDLPAQWPEFVGWAR
                     ALGVDGINVAARDLTWQLLHEWHDAGARVGVWTVDDPERIRAAAALGVDAITTNRPEV
                     ALRVVGELGLRARGGG"
     gene            1444..2490
                     /gene="trpS"
                     /locus_tag="PLS90_14550"
     CDS             1444..2490
                     /gene="trpS"
                     /locus_tag="PLS90_14550"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPK03665.1"
                     /translation="MTTPQKTILTGDRPTGPLHLGHYVGSLANRVRLQHEYRTFVLIA
                     DIQALTDNFDRPEIVRQNTLEVMLDYLAVGLDPERVTFVLQSLVPEIHELAIHYLNMV
                     TLARALRNPTVKSEIQERREREGGSDIFGEQFDIPLGFLIYPIHQAADITAFDADLVP
                     VGEDQLPMIEQAREVVRRINNYYGEGTLREPEGLVGEQPRLPGIDGKAKMSKSLGNAI
                     YLKDPAAEVERKVRMMFTDPNRVRADIPGRVEGNPVFIYHDAFNPNREEVEDFKARYR
                     AGKVGDVEVKNSLARALNEILEPMRARRADWEQRLPAVEQMLLDHTARARATSAAVLD
                     RLRERMGLRPLRAG"
     gene            2573..2980
                     /locus_tag="PLS90_14555"
     CDS             2573..2980
                     /locus_tag="PLS90_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03666.1"
                     /translation="MLANWLVPGLGFLLLGKRTRGLIQLGLVGVTFALGLLLDGGVAW
                     PAWSIRQPDFNLINNFTFLIQLGAGLPALVSLVAGLKGWTLLGGAPWASYFELGAYYL
                     VEAGAINYFAACNLYDRHVGGAARPGRSGDESA"
     gene            2977..3159
                     /locus_tag="PLS90_14560"
     CDS             2977..3159
                     /locus_tag="PLS90_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03667.1"
                     /translation="MTELGLIHLASAIYLSAVICLAVSVNENDEPMRIVRETLRRWAK
                     FMGIAAAIGVVVFLIG"
     gene            3423..4133
                     /locus_tag="PLS90_14565"
     CDS             3423..4133
                     /locus_tag="PLS90_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03668.1"
                     /translation="MIKRLKGFTLIELLIVVAIIAILAAIAVPNFLEAQVRSKVSRVR
                     ADIRSLATGIESYYVDNNIYPAQASSNPQDGAYGANSTVTNASQNLRNMPTFRLRVLV
                     QGSNPQRWDINTLTTPVSYITSYFSDPFADSKGATFSYSRDSFNRDGWIVWSYGPDTD
                     ERVTPTNTTHPVDNGPILDPSGGDIPVVLGAGGIPYPETTYYNPSFGHPSVRMTNIST
                     YDPTNGITSGGDVYRVKQ"
     gene            4701..>5381
                     /locus_tag="PLS90_14570"
     CDS             4701..>5381
                     /locus_tag="PLS90_14570"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPK03669.1"
                     /translation="MTESLAVTTLGEPRLDSPLRARDEGDGAVFHFVDEAARVPLEPR
                     AAAAQAGQASFEQAGPRERIYFDPARTRAAILTAGGLCPGLNDVIRALVLHLHYWYGV
                     HDVLGVPYGYDGLLPGNETPPRPLTLQAVSDIHTKGGSELGSARTPPDPEAWVDTLQA
                     LGVNVLFSIGGDGTLKGLHAISEEARRRGMALAAIGIPKTIDNDVPLVYRSFGFQTTV
                     EEADRVLDC"
BASE COUNT          894 a         1638 c         1900 g          949 t
ORIGIN      
        1 ggtgttgaag atgaaaggtc gtggttgacg ccgggggagc tgcgcaaccg ggggacgttc
       61 ctaagccgtc gtggccgggc ctccagatca cgtgcaaagg gaagccgcgg cggagaacaa
      121 cgtgaagcgc ggccagcatc gggccatcgg cggattcctg tcaagcgtta tttatcatgt
      181 gataaattcc ggctgggaga tcgccggcgc tatccgcctt gacgctgcgc gggggatcgt
      241 cgaaagtgcg gaggccgtcg cgaggcgccg cgccgtgctg caagttgacc gtgtttgcga
      301 tgcatctcga tcctggccgc gaggggtccg cccatgccgt tgtcgcgccg ccgccgcgcg
      361 ctcccacttt tgatcctgat tcttgttccg ccgctggccg ggtgcgccgg tctggccggg
      421 aggcagcccg tgcatacggt cgaagtgatc gcccaccgcg gcgcgagcgc ctcggcgccc
      481 gagaacacgc tggcggcgtt cacgcgagcc gccgatctgg gcgccgatgc cattgagctg
      541 gactgccggc tcacgcgcga cgggcaggcg gcggtaattc atgatgccgc gctcgaccgc
      601 gtcaacgcgc tggggggcga ggtcgccagt ttcccgctcg cagaactgca gacgatcgat
      661 gtcggttcat ggttcgggcc ggactggtcc gatcagacga tcccgtcgtt gggccaggcg
      721 ttggatctgg cgcgcgatcg cgggctgggg gtctacatcg aaatgaaggc ggaggcggag
      781 ccggcggggc tgatcgaccg gctgcgcggg gactacgccg gccggggccg gctgacgcgc
      841 gacgagctgg cgcgcgccgt gctgctgatc gagggattcc agtcgcccac ggcccggctg
      901 gtgcggcggg tcgtcgagga ggtccgcgcg cgcggaatgg aagagcaggt gctgctgcag
      961 tcgttctcgc cgatcgcgtg cgccgtggcg ctgcacgcgg cgccggaatt gccggtcttc
     1021 ctgatcggcg aaagcaatcc ggacctgccc gcccagtggc ccgagtttgt cggttgggcg
     1081 cgggcgctgg gggtcgacgg catcaatgtc gccgccaggg atctgacgtg gcaactgctg
     1141 cacgagtggc acgacgccgg agcgcgggtc ggggtctgga cggtggatga ccccgagcgg
     1201 atccgcgcgg cggccgcgct gggggtcgat gcgattacca ccaaccggcc ggaggttgcg
     1261 ttgcgggttg tgggtgaact ggggctgcgc gcccgcggcg gggggtgagg gcggaaagcc
     1321 cgggggagtc ggcggcttga catcagggcg cgcgttcttt agtgtcgcag aattctccgg
     1381 cgtgcccacc ggggacgata ggatcggaca ccttcgcgca ccttcgctcg gctggagcgg
     1441 ttcgtgacca ccccgcaaaa aaccatcctg accggcgacc ggccgaccgg cccgctgcac
     1501 ctgggccatt acgtcggcag cctggccaat cgcgtgcggc tgcagcacga gtaccgcaca
     1561 ttcgtactca tcgccgacat tcaggcgctg accgacaatt tcgaccggcc cgagatcgtc
     1621 cgccagaata cgttggaggt catgctggat tatctggccg tcggcctgga tcccgaacgg
     1681 gtgacgttcg tgctccagtc gctggtgccc gagattcacg agctggcgat tcactacctc
     1741 aacatggtca cgctcgcccg cgcgctgcgc aaccccacgg tcaagagcga gatccaggag
     1801 cgccgggaac gcgagggagg cagcgacatt ttcggcgagc agttcgacat tccgctggga
     1861 tttctgatct atcccatcca ccaggcggcc gatatcacgg cgttcgatgc cgatctggtg
     1921 ccggtgggcg aagaccagct cccgatgatc gagcaggcgc gcgaggtggt ccggcggatc
     1981 aacaactatt acggcgaggg gacgctgcgc gagccggagg ggctggtcgg cgagcagccg
     2041 cggctgccgg ggatcgacgg gaaggccaag atgagcaagt cgctgggcaa cgcgatctat
     2101 ctgaaggacc cggcggccga ggtcgagcgg aaagtccgga tgatgttcac cgatcccaat
     2161 cgggtccggg ccgacatccc cggccgggtg gaaggcaatc cggtattcat ctatcacgac
     2221 gcgttcaatc ccaaccgcga ggaggtggag gatttcaagg cacgctaccg cgcggggaag
     2281 gtgggcgacg tggaagtgaa gaacagcctg gcgcgcgcgt tgaatgaaat ccttgaaccg
     2341 atgcgggcgc gacgggcaga ctgggagcag cgcctgcccg cggtggagca gatgctgctg
     2401 gatcacacgg cgcgcgcgcg ggcgacgagc gccgccgtgc tggaccgcct gcgcgagcgg
     2461 atgggtttgc ggccgctgcg ggccggttag ttcccagttc caacgaggtc agccagcgcg
     2521 attgaaaacc cgtaacgcga tttgtcattc ccccccggcg gggtggggcc atgtgctggc
     2581 caattggctc gtgccgggtc tgggcttcct gttgctcggc aagagaacgc gtgggttgat
     2641 ccagctgggc ctggttggcg tgacgttcgc cctggggctg ctgctcgacg gcggggttgc
     2701 ctggcccgcc tggagcatcc gccagcccga ctttaacctg atcaacaatt tcacgttttt
     2761 gatccagctc ggcgccgggc tgcccgccct ggtgagcctg gtagcggggc tcaagggctg
     2821 gacgttgctg ggcggcgcac cgtgggcttc gtatttcgag ttgggcgcgt attatctggt
     2881 cgaggcgggc gcgatcaact atttcgccgc atgtaatttg tatgatcggc acgtcggggg
     2941 cgcggcgcgg cccggtcgct ccggggatga atcggcatga cggaactggg tctgatccac
     3001 ctcgcgtcgg cgatctatct gtcggcggtt atctgcctgg ccgtcagcgt caatgagaac
     3061 gacgagccga tgcggatcgt ccgcgagacg ctgcggcgct gggccaagtt catgggaatc
     3121 gccgcggcga tcggcgtggt tgtgtttctg atcggctgac cggggccgcg gaggggttgg
     3181 agtccaagtc ggtcgcgccc ggaacccaag gttgccgtcc gaaatccgtc gatccgaccg
     3241 atccgacgga ttttgacttt tgatgggggg tatgcggcgt gctaccccgc cggccggcac
     3301 gccgacgcag gggcgagaat ttcagcttga ctccgccacg ggcctttcct actctgatcc
     3361 tgctacaggc gctctacata ttccatatac ccaacccatg gtgagagaag gagggaactg
     3421 gtatgatcaa gcggttaaag ggctttaccc tgattgagct gctgatcgtc gtggcgatca
     3481 tcgccatcct ggcggcgatc gcggtgccga acttcctgga agcccaggtg cgttcgaagg
     3541 tctcccgcgt gcgggcggat atccggtcgt tggcgacggg tatcgagtcc tattacgtag
     3601 ataataacat ctacccggcg caggcgtcct cgaacccgca ggatggagcc tacggggcca
     3661 actcgacggt caccaacgcc agtcagaatt tgcgcaacat gccgacgttc cggctgcgcg
     3721 tcctggttca gggatccaat cctcagcggt gggacatcaa cacgctgacg acgccggtct
     3781 cgtacatcac gtcgtacttc agcgatccgt tcgccgactc gaagggggcg acgttcagct
     3841 actcgcgtga ctcgttcaat cgcgatggct ggatcgtctg gtcctacggc ccggacaccg
     3901 atgaacgggt gacgccgacc aacacgaccc acccggtcga caatggtccg atcctggatc
     3961 cgtcgggcgg cgacatcccc gtcgtgctgg gcgccggcgg catcccgtat ccggagacga
     4021 cctactacaa cccgtcgttc ggccatccgt cggtgcgtat gacgaacatc tcgacctacg
     4081 acccgacgaa cggcatcacc tcgggcggcg acgtttaccg cgtcaagcag taagcagcgc
     4141 agtcaggatt cgggctgggg tcctgcccag tccctggaaa ggccggctga gatcagccgg
     4201 cctttttctt tttttctcgc ttggggaatg agggcatcag cagggaccca agtgacggaa
     4261 gggacggaag ggaggcatgc aactcgaaga tttgtcctct attctcggga tcgctggggt
     4321 cggttctgcc gtccctgtgg gactctgtcg cgatggggat cccggtaccc gctgaagtag
     4381 cgggctgaaa tctgccgtcc ctgaggggac tggcctgctg aagagtccgg cagggcctgg
     4441 gttgttcgaa tcgacgttca gcccccattt tcatccctcg ctgggaaccc gccggttcaa
     4501 aacgaaagga cgaaagggct ggttttcgag agccgaggac gagggacgag agccgagagc
     4561 cgaaatctgg gtgggccgtt gggggcgagg acgaagacga aggatgattt ccggtggcat
     4621 ctgggacatg acaatcggca aatcctctgc cacgataggc ggttgtgaat gtgtccgcct
     4681 agcgcggcgg gagggaaatc atgaccgagt cgcttgccgt caccacgctg ggtgagcccc
     4741 gcctggactc gccgctgcgc gcgcgggacg agggcgacgg cgccgtgttc catttcgtcg
     4801 acgaagccgc gcgcgtcccc ctggagccgc gggccgccgc cgcacaggcg ggccaggcct
     4861 cgtttgaaca agccggaccg cgcgaacgga tatacttcga cccggcccgc acccgcgccg
     4921 cgatcctgac ggcgggcggc ctgtgccccg ggctgaacga cgtcatccgc gcgctggtgc
     4981 ttcacctgca ttactggtac ggcgtgcacg atgtgctggg ggtcccgtat gggtatgacg
     5041 ggctgctgcc gggcaatgag acgccccccc ggccgctgac gctgcaggcg gtcagcgaca
     5101 ttcacaccaa ggggggctcg gaactgggct cggcgcgcac gccgccggat cccgaggcgt
     5161 gggtcgatac gctgcaagcg ctgggggtca atgtgctgtt cagcatcggc ggcgacggca
     5221 cgttgaaagg gctgcacgcc atcagcgagg aggcccggcg gcgggggatg gcgctggccg
     5281 ccatcggcat ccccaagacg atcgacaacg acgtcccgct cgtctaccgc agtttcggtt
     5341 ttcagaccac cgtcgaggag gccgaccgcg tgctggattg c
//
LOCUS       DAOPCO010000273         5369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13050_length_5369_cov_5.306549, whole genome shotgun sequence.
ACCESSION   DAOPCO010000273 DAOPCO010000000
VERSION     DAOPCO010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13050_length_5369_cov_5.306549"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLS90_14575"
     CDS             <1..678
                     /locus_tag="PLS90_14575"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPK03670.1"
                     /translation="EVAMKAHAIETQKLRQQCGIQDQLSAAYGGILHIRMFKYPHAQV
                     SYLDVSNELRWELESRLALIFLGETHKSSEVHQWVIRQLEGSGPEHPTLCKLRGLAQA
                     GKNAIYEGDLNELGRLMIENTEAQAELHPDLVSPVARQTIEIARRHGAIGWKINGAGG
                     NGGSITLLLPSDWARRKDLIRAIEEIGGGVREIPIYLSRIGLRVWETRPGATRQTAGR
                     PPGDATA"
     gene            910..3630
                     /locus_tag="PLS90_14580"
     CDS             910..3630
                     /locus_tag="PLS90_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK03671.1"
                     /translation="MPAPGLKRWVGPLVLSGLAVAATAGQAPGEWLQWRRDAQRTGYS
                     PAAGRIVAPAIVGQAELGGVEDWLVLTPAAVGEAPVELPARLQPFDRAAARREWGLDR
                     WLDIEGTGEPEPVQERSRERYFQLDPNETRYDRVLFESTREKTMPATDTQRGWRERRV
                     DGQWQRLWTSEPDTVYEQPMVIRADADGDGREDVVVTVHYRIMAFDVETGEKIRELRY
                     SNYRNYGEVHAADLNGDGRPDFVQLVKFPAHLEVILNTAEGFEVAWFEDVMRGRITDT
                     ENSLRTPFNPIVDVDGDGRLDVVYVRYNQRGDGEWHTLVLDALTGETKHEIPGFVARE
                     IVNMGPDGRAWIFGTRSDGRFIPLRGGAAMHEIRDGEARLVWEAAELGWCDWLPNPWH
                     DARSASIYPKSRLAVSAETGLGWLHRAGTDGVEQLVLYRFAGDEGREVGAAGLPGGEA
                     VAFREQTGELLVRTTRPKADFNGQAWGLASRLVGTEELAGRVDAPVVFREADGAPRVV
                     VPDLLGGVMCLDLAQPDAPRTIWRRPGLGMGSGRGAVVARGEGDVRFPTFIEADARGA
                     ARVVQVDSAGETRWTVELPRTPARLNMEEEQGGIAQLMALPLQSAGVDDLAVTGLQSN
                     LHSGFTIALDGRNGEQLWRQERSGRVGVHNAEGVLEVEARGYGGNWPFAGLPKADGTE
                     DLVSAFPDIIYTADGRTGEFTLHHCTQFGTFMDYDAQGDVYRNKASFYAIPIVLPEGV
                     IYGGCPYVTGLWRVEDAFAGFQWRTAYHAPLYHRFPRPMQVAASLGEDGRRQLIGWEY
                     AKGMLTCRDLLEGDLRWTLAIAEGDAGDSFVGCDLDGDGRDEVLLCTPQELVAVGERE
                     GAGAILWRLALAAPCHEPVVADVDGDGAAEILLPCDDGRLYVVGNQGSGQ"
     gene            3659..>5369
                     /locus_tag="PLS90_14585"
     CDS             3659..>5369
                     /locus_tag="PLS90_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03672.1"
                     /translation="MKQACARIAMGILLLAGLAGPGAVSAQGGENEEAGRHWPMFRHD
                     FHLTGRSPLQLELATAPSVQWKHDLGAEQIAVETVTLEDFDGDHEHEALIRTNNSLKL
                     LDSDGSVLWEHAGLANPKVINQVDYAENGSLGIVYSASDGVKTTYSIIDSVSGTKTEL
                     FSMQNVFGLNMRHGKILPEIKGRQLVVFWSGDPVQGSIHPGQGYIWSFEEGCDKPKLL
                     FDKYVEGYILSPLLAFEDFNRDGKPDLYIVTHEHVYVWDILSGETLFEGYWNDDFIRN
                     YWANLAVYRFPGREYPSLLMINPMLPGAYVVDLVGEAPVRRWKQVIGANENEYQTVVE
                     VANGAPDPFVDLDGDGEPEILVRAKNEHLEDEEHLVIFNAEDGERIYDGPMVNILAVD
                     ELDGAAPPEVILNKETGLEIAHWTGEAFETVWTDGEAQPVLAPVDALQDPLRFVATRP
                     QFTNPVVWKSDAAEHAFYLRYADGVWSAKLTEGGAVEKIAPADPEHPATGFVPEGERM
                     RTTVESAEGVHRILRDGATVGEIAVTRERSFLSPPSIVGELGGQTRIVAQTADSRLVS
                     LDAA"
BASE COUNT          937 a         1481 c         2113 g          838 t
ORIGIN      
        1 gaggtggcga tgaaggcgca cgcgatcgag acgcagaagc tgcgccagca atgcgggatc
       61 caggatcaac tgagcgcggc gtacggcgga atcctgcaca tccggatgtt caagtatccg
      121 cacgcgcagg tctcgtatct cgatgtttcg aatgaactgc gctgggaatt ggagagccgg
      181 ctggcgttga tctttctggg cgaaacgcac aagtcgagcg aagtgcatca gtgggtcatc
      241 cggcagctcg aagggagcgg cccggagcac ccgacgctgt gcaagctgcg gggattggcg
      301 caggcgggca agaatgcgat ctacgaggga gacctgaatg aactgggccg gctgatgatc
      361 gagaacaccg aggcgcaggc ggaactgcac cccgatctgg tttcgccggt ggcgcgccag
      421 acgatcgaga ttgcgcgccg gcacggggcg atcggctgga aaatcaacgg cgccgggggg
      481 aacggcgggt cgatcacgct gttgctgccg tcggactggg cgcggcgcaa ggacctgatc
      541 cgcgcgatcg aggaaatcgg cggcggggtg cgggaaattc cgatctatct gagccggatc
      601 gggctgcggg tgtgggagac gcggcccggt gcgacgcgcc agacggcggg gcgcccgccg
      661 ggggatgcga cggcctgagc gcggtgggat ggccgggtgg tcaacgccgt gcaagaaaaa
      721 cccggatttg cgcaaagcat cgctctggcg ggttggacga ggcttcccgt atgctgtggc
      781 gatcagtgaa atgggcactt ccttgggcaa gcaacgcttc gaacggcacg ggccgggcgg
      841 cgaactccgc cggcctgggc gcgatgagac tgcagccagc ccatcgatcc aaagggaagg
      901 agcgagcgca tgcccgcgcc aggattgaag cggtgggtcg gcccgctggt gctgtcgggg
      961 ctggcggttg cggcgacggc gggtcaggcc cccggcgaat ggctgcagtg gcggcgcgac
     1021 gcccagcgga cggggtattc gccggcggcg ggacggatcg tcgcaccggc catcgtgggc
     1081 caggcggaac tggggggcgt ggaggactgg ctcgtgctga cgccggcggc cgtcggggag
     1141 gcgccggtcg agcttccggc ccggctccag ccgttcgacc gggcggccgc gcggagagaa
     1201 tggggcctgg atcgctggct ggacatcgag gggacgggcg aaccggaacc cgtgcaggag
     1261 cgcagccgcg aacgatactt tcaacttgat cccaatgaga cgcgttacga ccgggtctta
     1321 tttgaatcga cccgggagaa gacgatgccg gcgaccgaca cgcagcgcgg ctggcgggaa
     1381 cggcgcgtcg atggccagtg gcaacggctg tggacctcgg agcccgatac ggtctatgag
     1441 cagccgatgg tgattcgggc cgatgccgat ggcgacgggc gggaggatgt ggtcgtcacc
     1501 gtgcactacc ggatcatggc cttcgacgtg gagacaggcg agaagatccg cgaactgcgc
     1561 tactcgaact accgcaacta cggggaggtg cacgcggcgg acctgaacgg cgacggacgg
     1621 ccggacttcg tgcagctggt caagttcccg gcgcatctgg aggtgatcct gaacacagct
     1681 gaagggttcg aggtggcgtg gttcgaggac gtgatgcgcg ggcggatcac ggacacggag
     1741 aactcgctga ggacgccgtt caatccgatt gtcgacgtgg atggcgacgg tcggctcgac
     1801 gtggtgtatg tgcgctacaa ccagcggggc gacggcgagt ggcacacgct ggtgctggac
     1861 gcgctgacgg gggagacgaa gcatgagatc cccgggttcg tggcgcggga gattgtgaac
     1921 atggggcccg acgggcgggc atggattttc ggcacgcgca gcgacgggcg gttcattccc
     1981 ctgcgcggcg gcgccgcgat gcatgaaatc cgcgatgggg aagcgcgact cgtctgggaa
     2041 gccgcggagc tgggctggtg cgactggctg cccaatccgt ggcacgacgc gcgatcggcc
     2101 agcatctatc cgaagtcgcg cctggcggtg agcgccgaga caggcctggg ctggctgcac
     2161 cgcgcgggga cggacggcgt ggagcaattg gtgctgtacc ggttcgcagg ggatgagggg
     2221 cgcgaggttg gggcagcggg cctgcccggg ggcgaagccg tggccttccg agagcagacg
     2281 ggggagttgc tggtccggac gacgcgtccg aaggcggatt tcaacggcca ggcatggggg
     2341 ttggccagcc ggctggtcgg gacagaggaa ctggccggcc gggtcgatgc gccggtggtc
     2401 ttccgggaag cggacggcgc gccgcgcgtc gtcgtgccgg acttgctggg gggcgtcatg
     2461 tgcctggatc tggcgcagcc ggacgccccg cggacgatct ggcggcggcc cgggctggga
     2521 atgggttcgg ggcgcggggc ggtcgtggcg cggggcgaag gggacgttcg tttccccacg
     2581 ttcattgagg cggatgcgcg cggcgcggcg cgggtcgtcc aggtcgattc agcgggggag
     2641 acccgctgga cggtggagct gccgcgcacg ccggcgcgcc tgaacatgga ggaagagcag
     2701 gggggcatcg cgcagctcat ggcgctgccg ctgcaatcgg ccggggtgga cgatctggcg
     2761 gtgaccggac tgcagagcaa cctgcacagc gggttcacga tcgcgctgga cgggcgcaat
     2821 ggggagcagc tctggcgtca ggagcggagc ggtcgcgtcg gcgtgcacaa tgccgagggg
     2881 gtgctggagg tcgaggcgcg cggctatggc ggcaactggc cgtttgccgg cctgcccaaa
     2941 gcggatggga ccgaggatct ggtcagtgcg tttcccgaca tcatctacac ggccgacggg
     3001 cggacgggcg agttcacgct gcaccactgc acgcagttcg ggacgttcat ggactacgat
     3061 gcgcagggcg atgtctatcg gaacaaggca tcgttctacg cgatcccgat cgtgctgccc
     3121 gagggagtga tctacggcgg ctgtccgtat gtcacgggtc tgtggcgcgt ggaggatgcg
     3181 ttcgcggggt tccagtggcg gacggcgtat cacgcgccgc tgtaccaccg cttcccgcga
     3241 ccgatgcagg tggcggcgag tctgggggag gatggccggc gccagctcat tggctgggaa
     3301 tacgccaagg ggatgctgac gtgccgcgat ctgctggagg gggatctgcg gtggacgctg
     3361 gcgatcgcgg agggggacgc gggcgattcg ttcgtggggt gcgatctgga cggcgacggc
     3421 cgcgatgagg tgctgctgtg cacgccccag gaactggtgg cggtcggaga acgcgaggga
     3481 gccggcgcga ttctctggcg gctggcgctg gccgcgccgt gccacgagcc ggtcgtggcg
     3541 gatgtggacg gcgacggcgc ggcggaaatt ctgctgccgt gcgacgacgg gcgcttgtac
     3601 gtcgtcggca atcaggggtc tggtcaataa actcagtcga tcgcagaggg agcaagagat
     3661 gaaacaggca tgcgcgcgta tcgcgatggg aattctcctg ctggcggggc tcgccggccc
     3721 gggggcggtc tcagcccagg ggggggagaa cgaagaggcg ggtcgccact ggccgatgtt
     3781 ccgccatgac tttcatctca cgggccgctc gccgctgcag ctcgagctgg ccacggcgcc
     3841 gtcggttcag tggaaacatg acctgggggc cgaacagatt gcggtggaga ccgtgacact
     3901 ggaggacttt gacggcgacc acgagcatga ggcgctgatc cgaacgaaca actcgctcaa
     3961 gctgctggac tcggacggaa gcgtgctgtg ggagcatgcc gggctggcca atccgaaggt
     4021 gatcaaccag gtcgattacg cggaaaacgg cagcctgggc atcgtctatt cggcgtcgga
     4081 cggggtcaag acgacctatt cgatcatcga cagcgtgagc gggacgaaaa ccgaattgtt
     4141 cagcatgcag aatgtgttcg gcttgaacat gcgccacggc aagatcctgc cggagatcaa
     4201 ggggaggcaa ctggtcgtgt tctggagcgg cgatccggtc caggggagca tccatccggg
     4261 tcagggttac atctggagtt ttgaggaggg gtgcgacaag ccgaagctgc tgttcgataa
     4321 atacgtcgaa ggctacatcc tgagtccgct gctggcgttt gaggatttca accgggacgg
     4381 gaagcccgac ctgtatatcg tgacgcacga gcacgtctat gtgtgggaca tcctgtcggg
     4441 ggagacgctg ttcgagggct actggaacga tgacttcatc cggaactatt gggccaacct
     4501 ggcggtctac cgatttccgg ggcgggaata tccgtcgctg ctgatgatca atccgatgct
     4561 gccgggggcc tacgtggttg atctggtcgg cgaggcgccg gtgcggcgct ggaagcaggt
     4621 gatcggggcg aacgaaaatg agtatcagac ggtggtcgag gtcgccaacg gggcgcccga
     4681 tccgttcgtc gatctggatg gggacgggga gccggagatc ctggtgcgcg cgaagaacga
     4741 gcatctggag gacgaggaac acctggtgat cttcaacgcc gaggacggcg aacggatcta
     4801 cgacgggcca atggtcaaca tcctggcggt ggatgagctg gacggggcgg cgccgcccga
     4861 agtgatcctg aataaggaaa cgggactgga gatcgcgcac tggacgggcg aggcgttcga
     4921 gacggtctgg accgacggcg aagcgcagcc ggtgttggcg ccggtcgacg cgctgcagga
     4981 cccgctgcgg ttcgtcgcga cgcgcccgca gttcaccaac ccggtcgtct ggaagtcgga
     5041 tgcggcggag cacgccttct atttgcgtta cgccgatggc gtctggagcg cgaaactgac
     5101 ggaggggggc gcggtggaaa agatcgcgcc ggccgatccg gagcatccgg cgaccggatt
     5161 cgtgcccgaa ggggagcgga tgcggacgac ggtcgagtcg gcggagggcg tgcatcggat
     5221 tttgcgcgac ggagcgaccg tcggggaaat cgcggtgacc agggaacgca gcttcctgtc
     5281 gccgccgtcg atcgtcggcg agctgggagg ccagacgcgc atcgtcgccc agacggccga
     5341 ttcgcggctg gtgtcgctcg acgccgcgg
//
LOCUS       DAOPCO010000274         5323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13183_length_5323_cov_2.995065, whole genome shotgun sequence.
ACCESSION   DAOPCO010000274 DAOPCO010000000
VERSION     DAOPCO010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13183_length_5323_cov_2.995065"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="PLS90_14590"
     CDS             <1..1163
                     /locus_tag="PLS90_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03673.1"
                     /translation="TSKICQAVSSICSYETSSNLVAALADHFMEYAGIILRHYPWIPI
                     GLLLLVFCYFRRPVIILYVAFIVILYLVVADGSYKIQGRFSDSAHLFSRGYAEHAVRP
                     VFLMLGLPICLRRLQARPARIVLGGLLLLLFLTFFIETAGAAKEYANRKDDIQHLVRA
                     PDLRERSAALVGFQNLKNFLSNDTDWFLYETLIYSSLLPDGSPRMILISDTVPNAAQL
                     RSYLPHGINTRIFPIKLAPTGKIDVVNMRSDLSLEDLAHIDPAAIHVEWEVPVKGVIS
                     KQIRVIANIQNRNSIPLKSGLDGDASIYLFPVWERSGAVYLRNRPLPLHLDIHTQYNQ
                     IINVDVPDDPGNYLLKLYLMARVEPRADETNYTCRNFARGVFAVEVRNGNED"
     gene            1167..2438
                     /locus_tag="PLS90_14595"
     CDS             1167..2438
                     /locus_tag="PLS90_14595"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPK03674.1"
                     /translation="MPLKIGLIGATLWGNRGAEAMLVTTIGRLRRTFPDARFVIFSYS
                     PSKDREILADPTIAVAAASPKALVLAHFPWAVIDRLLRSIGLRPPRGLLPRSARELRE
                     LDVLLDLFGVSYADGRERFLPFNVLSNWPAMLFGVPVVKLAQSAGPFHNPLNRWIGRW
                     MLGKCEKIHARGEATRRYLDELGIASDPSPDVAFLYEPDYSLTEENPDVIANVRAQLA
                     AWKASGHPIVGFSVSKVVKNMCDAHGMDYCAILSAAAVDLIDHGRRVVFLPNATRGSE
                     LARTLNDLPIIEQVLETLRTRGAPYAEAATGIRVDVNTRGLRQVISECDVLVASRFHG
                     MIAALCEEVPLLVVGWGHKYEDTLREFGLAELSIDFSSLKPGDLAAGVGSLLERREQY
                     RLQIRRHLPEVKRAADQAFNWLEGFLKARAR"
     gene            2435..3406
                     /locus_tag="PLS90_14600"
     CDS             2435..3406
                     /locus_tag="PLS90_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03675.1"
                     /translation="MKSKAAACLPFVWRWTKIAWLLACLVLLVAYGRRQWDEIAPLAA
                     DIHWFYLLPALALIVAGRIALSTAAREICLWSGTSFRHSQMLKIYAVTQLAKYVPGGV
                     WHFVARGIAYSEAKMKTRRIAQNIVVENLILLGGAVCVGLVGMLPTSPGLAFVAPPIR
                     FLTIGAALLIYPVAVYGAAAIIRRDVPARSRLAVVLFQLGAWLPIGAAFHFMIVALGI
                     SHVPPAYAAGTFTLCWAIAFVAVFVPGGIGLREALLVYMLGPFMPASAAFTVAVLSRI
                     VWTVAEAALLLVALVWTAAERRVSHLPLAPVPCLTTIEESNPPNNGG"
     gene            3420..4769
                     /locus_tag="PLS90_14605"
     CDS             3420..4769
                     /locus_tag="PLS90_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPK03676.1"
                     /translation="MPARNLQPVIDHSMCIGCGACAAADPSVSIVFNDFTQMYQPDSP
                     GGEEAAAVCPAVQVDFEFLQRRLFPGQPPGPLGVLEKAFLAQSRDYQRNLNSSSGGLI
                     KELLHLLIRRDDIDGVICLQHVDRLVYEPRLLTDPDQIDRLGGSIYHNISFANALRLL
                     RENTGRYVLVAIPCQLEGIYSYIFKQAPELRERIAFTIGLLCGWTFSHHALRAICDFK
                     GIPFDSIEMVTYRGGGPVGPLRIQAGGRLHKIHRRIDLDYQVAFDRSFNLPRCHLCIN
                     HGNFLADIVVGDAWLPSTVRTRTGISLLLCRARESVARVEELAAAGRIVHVEATADDV
                     LESQTRRSAYGDFAYALADCLKSQGQFVPDMTGPNRVAAHLADPDDAASYHRAYLKHR
                     RLQFERKYRALRLRKLTVGLPQISRRYFRWFFIRLLKIDRVFRRERKPVPKHMEIFR"
     gene            complement(4810..>5323)
                     /locus_tag="PLS90_14610"
     CDS             complement(4810..>5323)
                     /locus_tag="PLS90_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03677.1"
                     /translation="SVSEDGTPDADDRDYGGGTLAPLPVDPLDDPSFEIWDTTLVEWQ
                     VLAGELSLTTDTTHGASAVELRAPLDAAGSLRQKLDADVELAGRQVTASVDAKCTTPD
                     AVFLVLEYRGANRWVPVNSLMHPGDGNWSRLEVSQQIPEDVPGGGVAFKLIVRPGARA
                     IFDNAVLAVR"
BASE COUNT          909 a         1721 c         1548 g         1145 t
ORIGIN      
        1 gtacaagtaa aatatgtcaa gcagtctcaa gcatttgcag ttatgaaacg agttcgaatc
       61 ttgtcgccgc gctggccgat cacttcatgg agtatgccgg tattatcctc cgccactatc
      121 cctggatccc gattggactg ctgctgcttg ttttctgcta tttccgtcgg cctgttatta
      181 tcctttatgt agctttcata gttatcttgt acctggtggt tgccgatgga tcgtacaaaa
      241 tccaggggcg tttttccgac agtgcccacc tgttcagtcg gggctatgcc gagcatgccg
      301 tgcgccccgt cttcctcatg ctcggcttgc cgatctgcct tcgccgtctt caagcgcgtc
      361 ccgcacggat cgtgctgggg ggactcctgc tcttgctttt cctcaccttt ttcattgaga
      421 cggccggcgc cgcgaaggaa tatgcgaatc ggaaagacga catccagcat ctcgtgcgcg
      481 cacccgatct acgggaacgt tccgcggctc tggttggctt tcagaatctg aagaatttcc
      541 tgagcaacga taccgactgg ttcctctatg aaacgctgat ctacagctcc ctgctgccgg
      601 acggctctcc ccggatgatt ctgatatccg acacggtgcc gaacgccgcg caattacgct
      661 cgtatctgcc acacgggatc aatacccgca ttttcccgat caagcttgcg cccacgggca
      721 agatcgatgt cgtcaatatg cgctcggatc tgtcgctcga agatcttgca cacattgatc
      781 cagcggcaat tcatgtcgag tgggaagtcc ccgtgaaggg cgtgataagc aagcagattc
      841 gcgtgattgc caacatccag aatcgtaatt ccattccgct gaaatcaggc ctcgacggcg
      901 atgcgtcgat ctatcttttt ccggtctggg aacgctctgg cgccgtctac ctccgcaacc
      961 gacctctgcc gcttcatctc gatatccaca cgcaatacaa ccagatcatc aatgtcgacg
     1021 tgcccgatga tcccggcaat tatctgctca aactctatct gatggcgcgc gtcgagccgc
     1081 gcgcggacga gacgaactat acctgcagga attttgcgcg cggcgtcttc gcggtcgaag
     1141 ttcggaatgg aaatgaggat taggcgatgc ctctgaaaat tggcttgatc ggcgcgacgc
     1201 tgtggggcaa tcgcggggcg gaggcgatgc tggtgacgac gatcggccgg ctgcggcgaa
     1261 cgttccccga cgcccgcttc gtcatcttct cctattcccc atcgaaggac cgcgagatcc
     1321 tggcggatcc cacgatcgcc gtcgccgcgg cctcgcccaa agccctcgtc ctcgcgcact
     1381 ttccctgggc ggtcatcgat cgactgttgc gctcgatcgg gctgagaccg ccgcgcgggc
     1441 tccttccgcg cagcgcccgc gagcttcgcg agctggatgt tctgctcgat ctgttcggtg
     1501 tctcctacgc cgatggccgc gaacggtttc tccccttcaa tgtgctctcc aactggccgg
     1561 cgatgctttt tggcgtgccc gtcgtcaagc tggcgcagag tgccggcccg ttccacaacc
     1621 cactcaatcg ctggatcggc cgctggatgc tcggcaaatg cgagaagatc cacgcgcgcg
     1681 gtgaagcgac tcgccgctat ctggacgaac tgggcattgc cagcgatccc tcgcccgatg
     1741 ttgcctttct ctacgaaccg gattactcgc tgaccgaaga gaaccccgac gtgatcgcga
     1801 acgtgcgagc gcaacttgcc gcatggaaag cgtcagggca tccgatcgtc ggcttttcag
     1861 tcagcaaggt cgtcaagaat atgtgtgatg cccatgggat ggattattgt gctatcctgt
     1921 cagcggccgc ggtcgatctg attgatcacg gccggcgtgt ggtcttcctg ccgaatgcga
     1981 cgcgcggatc cgagctcgcc aggacgctga atgatctgcc gatcatcgag caggtcctcg
     2041 agaccttgcg gacccggggc gctccatacg cggaagcggc cacgggcatc cgggtcgatg
     2101 tcaatacgcg ggggttgcgc caggtgattt cggaatgcga cgtcctggtg gcctcgcgat
     2161 tccacggcat gattgcggcc ctgtgcgagg aggttccgtt gctggtggtc ggttgggggc
     2221 ataaatacga agacaccctc cgcgaatttg gccttgcgga gttgtcgatc gacttttcct
     2281 ccctcaaacc aggcgacctc gccgcaggcg tcggatcgct gctggagcgc agggagcagt
     2341 atcgactcca gatccgacgg catctgcccg aggtcaaacg cgccgcggac caggccttca
     2401 actggctcga gggcttcttg aaagcgcgag cgagatgaag tccaaagccg cggcctgcct
     2461 tcccttcgtc tggcgctgga ccaagatcgc ctggttgctg gcttgcctcg tcctgctggt
     2521 cgcctacggt cgccgtcaat gggatgaaat cgcgccgctt gccgccgaca tccattggtt
     2581 ttacctgttg ccggcgctgg ccctgatcgt cgccggccgt atcgcgctgt cgaccgcggc
     2641 ccgcgaaatc tgtctgtggt ccggcaccag cttccgtcat tcccagatgc tgaaaatcta
     2701 cgccgtgacc caactggcga agtatgtgcc gggcggggtc tggcattttg tcgcgcgcgg
     2761 catcgcctac tccgaagcca agatgaagac gcggcggatt gcccagaaca tcgttgtgga
     2821 aaacctgatt ctgctcggcg gcgccgtctg cgtcggtctt gtcggcatgt tgccgacttc
     2881 tccagggctg gcattcgtcg cgccgcccat ccgcttcctg acgatcggcg cggcgctgct
     2941 gatctatccg gtcgccgttt acggcgcggc ggccatcatc cggcgcgatg tcccggcccg
     3001 ttcgcgtttg gcggttgtcc tgttccagct cggggcctgg ctcccgatcg gcgccgcgtt
     3061 tcatttcatg atcgtggcgc tcgggatctc gcatgtgccg ccagcgtatg cggcgggaac
     3121 gttcacgctc tgctgggcga tcgcctttgt cgccgttttc gtgccgggcg gcatcggact
     3181 gcgcgaggcg ctgctggtct acatgctcgg tccgttcatg ccggccagcg ccgcgttcac
     3241 cgtggcggtc cttagccgca tcgtctggac cgtggccgag gccgccctgt tgctggttgc
     3301 gctggtctgg acggcggctg aacgccgtgt gtcgcacctc ccccttgcac ccgttccctg
     3361 tctgaccaca atcgaagaga gcaacccgcc caacaacgga ggatgaacga ctcaatccga
     3421 tgcctgcccg caaccttcag cccgtgatcg accattcgat gtgcatcggc tgtggcgcgt
     3481 gcgccgcggc cgacccctcg gtttccatcg tattcaatga tttcacgcag atgtatcagc
     3541 ccgattcccc gggcggcgaa gaggcggcgg cggtttgtcc cgccgttcag gtcgattttg
     3601 agtttctgca gcggcgactt ttcccgggcc agccgcccgg accgctgggc gtcctggaaa
     3661 aggccttcct cgcccagagc agggactatc agcgtaatct caactcgagc tccggcggct
     3721 tgatcaagga attgctgcac ctgctcatcc ggcgcgacga catcgacggc gtcatctgcc
     3781 tgcaacacgt cgaccgtctg gtttatgagc cgcggctgct caccgacccc gaccagatcg
     3841 accgccttgg cggctccatt tatcacaata tctcgttcgc caacgcgctg cggctcttac
     3901 gggaaaacac gggccgctat gttctggtgg cgatcccatg tcagctcgag gggatttaca
     3961 gttacatctt caagcaagcg cctgaactgc gcgagcggat tgcctttacg atcggcctgt
     4021 tgtgcggctg gaccttctcg caccatgccc tgcgcgcgat ctgtgatttc aagggcattc
     4081 ccttcgattc catcgaaatg gtcacctacc gcggcggagg acccgtcggc ccgttgcgca
     4141 ttcaggccgg cggcaggctt cacaagatcc accggcgcat cgatctggat tatcaggtgg
     4201 cgttcgatcg ctcgttcaat ctcccgcgct gccatctatg catcaaccat ggcaatttcc
     4261 tggccgatat cgtcgtgggc gatgcctggt tgccctccac ggttcggacc cgcaccggca
     4321 tcagcctgct cctctgtcgc gcacgtgaat cggtcgcacg ggtcgaggaa cttgccgcgg
     4381 ccggtcgcat tgtgcatgtc gaggcgactg cggacgacgt tctagagtca caaacccggc
     4441 gcagtgccta cggagatttt gcctatgccc ttgccgattg cctcaaatca caaggtcaat
     4501 tcgttcccga catgacggga cccaaccgcg tcgccgccca ccttgccgac ccggacgacg
     4561 ccgccagcta ccatcgcgcc tatctgaagc atcggcggct ccagttcgag cggaaatatc
     4621 gggcgttgcg cttgcggaaa ttgaccgtgg ggttgccgca gatcagccgg cgttactttc
     4681 gctggttctt tattcgcctg ctcaaaatcg accgcgtctt ccggcgcgaa cggaaacccg
     4741 tccccaagca catggagatc ttccgctaaa cgactcccga catcggttga gcgcgagaac
     4801 tgtgcccggc taccggacgg ccaggacggc attgtcaaat atggcgcggg cgccggggcg
     4861 gacaatcagc ttgaaggcaa cgccgccgcc gggaacatcc tccgggatct gctggctgac
     4921 ctccaggcgc gaccagttgc cgtcgcccgg atgcatcagg ctgttgaccg gaacccagcg
     4981 attggcgccg cgatactcca gcacgagaaa caccgcatcc ggcgtcgtgc atttcgcatc
     5041 cacggaggcc gtcacctgcc ttcccgccag ttccacgtcc gcatcgagtt tctgccgcag
     5101 actccccgcc gcgtccagcg gagcgcgcag ttccaccgcc gaagccccgt gcgtggtatc
     5161 ggtcgtcagc gacaattcgc ccgcgagcac ttgccactcg accagcgtcg tatcccagat
     5221 ttcgaacgag ggatcgtcca agggatccac cggcagcggc gcgagcgtcc cccccccgta
     5281 atcgcgatcg tccgcatcgg gggtgccgtc ctcggaaacg gac
//
LOCUS       DAOPCO010000275         5284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13291_length_5284_cov_5.230063, whole genome shotgun sequence.
ACCESSION   DAOPCO010000275 DAOPCO010000000
VERSION     DAOPCO010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13291_length_5284_cov_5.230063"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="PLS90_14615"
     CDS             complement(<1..1331)
                     /locus_tag="PLS90_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03678.1"
                     /translation="MPLSGLLSSISLDPSGRLESIQFPGTAPRSILDLGVRITLDSGV
                     ITFADCSIEQLASDQWRASYRNHVVGLDLHLRYEAVASGGFVRKHLTVVNRGSRRHLI
                     QRIDLWDLHFTPAFQQSLPLFDPAAHPCPLGLSLRDAEQGLSVGLEHPFFRMSVDPGH
                     CQIWYEPNAWLAPEESLACEAGFMGRWRPSGKLRALATLNGEEDAADEAELAALAEYL
                     RRIGPVWRREPFGSRDENGSENGGERASASSIFPNAPVAGGEPDPRILELNGARLGTA
                     CWADARQGERFLAECLERLSQGGPRAIRFIDGWFDGRFSPAGEDGSAAGVPREPHPDC
                     YDPEHAHPRGVCRYLAWRNVARVLGELKRRHPDCRFEFAGGNRRLGPWLTGELTLGEG
                     AAETPAGALSEADRSIYMFVPHDHMLDEDSLLETAEGGIAAKTAVVSVDVL"
     gene            1692..2999
                     /locus_tag="PLS90_14620"
     CDS             1692..2999
                     /locus_tag="PLS90_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03679.1"
                     /translation="MESLWLGTARADISPRSPLPVAGFGFRRGVSRGVIRPLQARIVC
                     FSPGADAPPARRVLLISADILGWGDDQLERLGARLARDCGLPAEALILHATHSHSGPQ
                     LCQHVLPCLGVRDDQYAAWMEDQVIEASRRALASLEPARMELHLGECAIGVHRRRLRD
                     GCWRMAPNPDGPVDHEVRVIRCIAPDDSTRALLVHFACHPTASADDFFSPEYPGLAMT
                     ALENEPRGPAMALFLQGCCGDIRPAMIRDGEFYRGDFNDAERLAARLADAVRQALARP
                     GQPLPLDELETERTERALPLGPLPTPEQLRDRADRQTVEGEWSRHLLAHPAAIRPHAP
                     LRLTLLRLSPALRLIFANAELMIEHGLEFKRRSDGRDLLVGCSNGVLGYLPTARQLEE
                     GGYEPAESWLYYGAPAPFTSDAEQVFRSAVEQLVTAGEGEHVA"
     gene            2989..3750
                     /locus_tag="PLS90_14625"
     CDS             2989..3750
                     /locus_tag="PLS90_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPK03680.1"
                     /translation="MSPDSPTTRYLEIVLRHLRRLHDEEQPALRQAAALVADQVSRDR
                     LVYVFGPGGHSNLGSQEVFFRAGGLMHIDAILDEGTLLSNGALRSMAIERLPGYGRIV
                     IRDYGIAAGDLLIIVNAYGINAATIDAALEARERGIRTIGVTSVEHARQTPPEHPARH
                     PSRQNLHDLVDVVLDCKIPVGDAVVEIEGLDQRVAALSTFANAFLLNALVAETVELLA
                     RDGIRPPIWTSGNATGGDEANARFIHRFKGRIRKL"
     gene            3788..>5284
                     /locus_tag="PLS90_14630"
     CDS             3788..>5284
                     /locus_tag="PLS90_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03681.1"
                     /translation="MPTFSNSIAPADYLILAGFFGLMLVIGFYFAGRMRDLGDYFSGG
                     RRVPWWLAGTSYYMSSFSAFAFVAYSALAYEHGWVAVTLYWVSVPAALVSVALVAARW
                     RRAATTSPVEFLEERYGRFMRQSLAWLGIPVKIIDDGLKLFAIGLLVSTGLGIRHPHA
                     LAWGILGSGLIMLLYTLMGGLWAVLVTDFIQFLVMIAAVLVLFPLALSRVGGLDAFVD
                     GSPDGFFRLTSAEFGPGYIVAFLFIMIFSYSTQWQLVQRFYIVPRDADARRVGYLVAA
                     LNLVTPPLFFAPAMMARQFLPGVDDPANIYALLCRELLPVGMTGLIVAAMFAATMSML
                     SSDYNAVAAVLTNDVYRRLIAPAASGRVLVRVGRLTTLIAGLLSLAVAFMIVASEGES
                     GLFDRMVSLFSLFLPPIAIPMLAGLLTRRVSNAGAVLGLAAGILVGLAAYIIRTTLAA
                     PPDADPATFTAAQRLAALLRGNEVMTALTASTALLGLGIGSWLRPDPAA"
BASE COUNT          753 a         2071 c         1584 g          876 t
ORIGIN      
        1 atcagcacgt ccaccgagac gacggccgtc ttggccgcga tccccccctc ggccgtctcc
       61 agcagcgaat cctcgtccag catgtgatcg tgcggcacaa acatgtaaat cgagcgatcg
      121 gcttcgctca aggccccggc cggggtttcc gccgcgcctt cgccgagcgt cagctcgccg
      181 gtcagccagg gaccgagtcg ccggttgccc cccgcgaact cgaaccggca atcgggatgc
      241 cgccgcttca gctcgcccag cacgcgcgcc acgttgcgcc acgccaggta gcggcacacc
      301 ccgcgcgggt gcgcatgctc cgggtcgtag caatccggat gcggttcgcg cggcacgccg
      361 gccgcgctcc cgtcctcccc cgccgggctg aaccgcccgt caaaccagcc gtcgatgaac
      421 cggatcgcgc gcggcccgcc ctgactcagc cgctccaggc actcggccag gaaccgctcg
      481 ccctgacgcg cgtccgccca gcaggccgtt cccagccgcg cgccgttcag ttccagaatc
      541 cgcgggtcgg gttcaccgcc ggcgacgggt gcattgggaa aaatcgagga ggccgacgcc
      601 cgctcgccgc cgttttccga cccgttctca tcgcgcgagc caaacggttc gcgccgccag
      661 accggtccga tccgccgcag atactcagcc agcgcggcca gttccgcctc gtcggcggca
      721 tcctcctcgc cgttgagcgt cgccagcgcc cgcagcttgc ccgacggccg ccagcggccc
      781 atgaatcccg cctcgcaggc gagcgattcc tccggcgcga gccacgcatt cggttcatac
      841 cagatctggc aatgccccgg gtcgaccgac atccggaaga acggatgctc gagacccacg
      901 ctcagcccct gctccgcatc acgcagcgac agccccagcg ggcagggatg ggccgccgga
      961 tcgaacaacg gaagcgactg ctgaaacgcc ggcgtgaaat gcaggtccca cagatcgatg
     1021 cgctggatca ggtggcgccg cgacccccga ttcaccaccg tcagatgctt gcgcacgaac
     1081 ccgccggagg cgacggcttc gtagcgcagg tgcagatcca gcccgacgac gtgattgcgg
     1141 tagctggcac gccactggtc gctcgcaagc tgctcaatcg agcagtcggc aaacgtgatc
     1201 acgcccgagt ccagcgtgat gcgaaccccg aggtcaagga tcgaacgggg cgccgttccg
     1261 ggaaactgga tcgattccag gcggccgctg ggatcgagcg agatggagga aagcaatcct
     1321 gaaagaggca tgagcgaggc tttggccggg gcggcggaat cggaggggcg agataatcgt
     1381 ttatccgaaa tcgttttccc acgatccccg actcgctgtc aagcacgaat ggtgcagagc
     1441 ccaagtggtc aagataagac ctcgcagtct gtaaagaaaa taatgaactc ggcggacccg
     1501 ccgtgacccg gccgcgaccc ggtccccatt gtccgccagc tcgcttatgt cccttatgtc
     1561 ctttctgcct ctgaattctt tcatctctcc gatttttccc agaccgacct tgactccagc
     1621 acccggcgcg gggtaaatga ataccgctaa tcgttttcgc acgccccccg gtccgccctc
     1681 aggagccgtc catggagtca ctctggctgg gtaccgcccg cgccgacatc tccccccgct
     1741 cgcccctccc cgtggccggg ttcggcttcc gccggggcgt ctcgcggggc gtcatccgcc
     1801 cgctccaggc gcgcattgtc tgtttctccc ccggcgccga cgcccccccg gcgcgcaggg
     1861 tcctcctgat ctccgctgat atccttggct ggggcgacga tcagctcgag cgtctcggcg
     1921 cccgcctcgc gcgcgattgc ggcctccccg ccgaggcgct gatcctccac gccacgcact
     1981 cccattccgg cccccagctg tgccagcacg tcctcccctg cctgggcgtc cgcgacgacc
     2041 aatacgcggc ctggatggag gatcaggtca tcgaggcgtc gcgccgggcc ctcgcttcgc
     2101 tcgagcccgc ccggatggag ctccacctgg gcgagtgcgc gatcggcgtc catcgccgcc
     2161 gcctccgcga cggctgctgg cggatggccc ccaatcccga cgggcccgtc gatcatgagg
     2221 tgcgcgtcat tcgctgcatc gcccccgacg actcgacgcg cgcgctgctg gtccatttcg
     2281 cctgccatcc caccgccagc gccgacgact tcttctctcc cgaatacccc ggcctggcca
     2341 tgaccgccct cgagaacgaa ccgcggggcc ccgccatggc cctctttctg cagggttgct
     2401 gcggcgacat ccgcccggcg atgatccgcg acggcgaatt ttatcgcggc gatttcaatg
     2461 acgccgagcg tctcgccgcg cgcctcgccg atgccgttcg ccaggccctc gcgcggccgg
     2521 gccagcccct cccgctcgac gagctcgaga ccgaacgcac cgagcgcgcc ttgccgctcg
     2581 gaccgctccc caccccggag caactccgcg accgggccga ccggcagacg gtcgagggcg
     2641 aatggagccg ccacctgctc gcccatcccg cggcgatccg tccgcacgcc ccgctccggc
     2701 tcaccctgct gcgcctctcc ccggcgttgc ggctcatctt cgccaacgcc gagctcatga
     2761 tcgaacacgg cctggagttc aagcgccgct ccgacggccg cgatctgctg gtgggctgct
     2821 ccaacggcgt cctgggctac ctccccaccg cgcgccaact ggaggaaggc ggctatgagc
     2881 cggccgagtc ctggctttac tacggcgcgc ccgcgccgtt cacatccgac gccgaacagg
     2941 tcttccgctc cgcggtcgag cagcttgtga cggcggggga gggcgagcat gtcgcctgat
     3001 tcgccgacca cgcgctacct ggaaatcgtg ctccggcacc tgcgccggct gcacgacgaa
     3061 gagcaaccgg cgctgcgcca ggccgccgcc ctggtcgccg accaggtcag ccgcgaccgc
     3121 ctggtctacg tcttcggtcc cggcggccac tccaacctcg gctcgcagga agtcttcttc
     3181 cgcgccggcg gcctgatgca catcgacgcc atcctcgacg aggggacgct gctgagcaac
     3241 ggcgccctgc gctcgatggc catcgagcgg ctgcccggct acggccgcat cgtcatccgc
     3301 gactatggca tcgcggcggg cgacctgctg atcatcgtca acgcctacgg catcaacgcc
     3361 gcgacgatcg acgccgcgct cgaagcccgc gaacgcggca tccgcaccat cggcgtcacc
     3421 tccgtcgaac acgcccgcca gacccccccc gagcatcccg cgcgccaccc ctcgcgccag
     3481 aacctccacg atctggtcga cgtcgtcctg gactgcaaga ttcccgtcgg cgacgcggtc
     3541 gtcgagatcg agggcctcga tcagcgcgtc gccgccctct ccaccttcgc caatgcgttc
     3601 ctgctcaacg cgctcgtggc cgagaccgtc gagctgctcg cccgcgacgg catcaggcct
     3661 cccatctgga ccagcggcaa cgccaccggc ggcgacgagg ccaatgcccg cttcatccac
     3721 cgcttcaagg gccgcatccg caagctctga tttccgcgcc gcgcccccga ccccggagcc
     3781 ctcgcccgtg ccaaccttct ccaacagcat cgcccccgcc gattatctga tcctggccgg
     3841 cttcttcggc ctgatgctgg tcatcggatt ctacttcgcc gggcgcatgc gcgatctcgg
     3901 cgattacttc agcggcggcc ggcgcgtccc ctggtggctc gcgggcacct cctactacat
     3961 gagcagcttc agcgccttcg ccttcgtggc ctactccgcg ctggcctacg agcacggctg
     4021 ggttgccgtc acgctctact gggtctcggt gccggcggcg ctggtcagcg tcgcgctggt
     4081 cgcggcgcgc tggcggcggg ccgccaccac cagcccggtc gaattcctcg aggagcgtta
     4141 cggtcgcttc atgcgccaga gcctggcctg gctcggcatc cccgtcaaga tcatcgacga
     4201 cggcctcaag ctgttcgcca tcggcctgct cgtctccacg ggcctcggca ttcgccatcc
     4261 gcacgccctc gcctggggga tcctcggctc cggcctcatc atgctcctct acacgctgat
     4321 ggggggcctc tgggccgtcc tcgtcaccga cttcatccag ttcctcgtga tgatcgcggc
     4381 ggtgctcgtg ctcttcccgc ttgccctgag ccgtgtgggc ggcctcgacg ccttcgtcga
     4441 tggttcaccc gacgggttct tccgcctcac ctccgccgaa ttcggcccag gctacatcgt
     4501 cgccttcctg ttcatcatga tcttcagcta cagcacgcag tggcagttgg tgcagcgctt
     4561 ctacatcgtc ccgcgcgacg ccgacgcccg gcgggtcggc tatctggtcg ccgcgctcaa
     4621 cctcgtcacg ccgccgctct tcttcgcccc cgcgatgatg gcgcgccagt ttctccccgg
     4681 cgtcgacgat ccggccaata tctacgccct cctctgccgc gagctcctgc cggtggggat
     4741 gaccggcctg atcgtggcgg cgatgttcgc cgccacgatg tcgatgctct cgagcgacta
     4801 caacgccgtc gccgccgtgc tgaccaacga cgtctaccgc cgcctgatcg cccccgccgc
     4861 gtccggacgc gtcctcgtgc gcgtcggccg gctgaccacg ctgatcgccg gcctcctctc
     4921 gctcgccgtg gcgttcatga tcgtcgcctc cgagggggaa tccggcctct tcgatcgcat
     4981 ggtgtcgctc ttcagcctgt tcctgccgcc gatcgcgatc ccgatgctgg ccggcctgct
     5041 gacccggcgc gtgagcaatg cgggggccgt tctcggcctc gccgccggca tcctcgtcgg
     5101 tctggccgcc tacatcatcc gaacgacgct ggcggcgccg cccgacgccg accccgcaac
     5161 cttcaccgcc gcgcagcggc tcgcggccct gctccgcggc aacgaggtca tgaccgcgct
     5221 gaccgcctcg accgcgctgc tcggcctggg gattggctcc tggctgcgac ccgatcccgc
     5281 ggcc
//
LOCUS       DAOPCO010000276         5257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13376_length_5257_cov_4.313149, whole genome shotgun sequence.
ACCESSION   DAOPCO010000276 DAOPCO010000000
VERSION     DAOPCO010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_5257_cov_4.313149"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..318
                     /locus_tag="PLS90_14635"
     CDS             34..318
                     /locus_tag="PLS90_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPK03682.1"
                     /translation="MIDYQAGAIVSRQILKQKTGSVTLFAFDAGESLSEHTTSFDAVA
                     LVLDGRARITIGGAPHVVATGQSILMPAGIPHAVAADERFKMLLMMLRGE"
     gene            complement(492..2675)
                     /locus_tag="PLS90_14640"
     CDS             complement(492..2675)
                     /locus_tag="PLS90_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03683.1"
                     /translation="MGLALAWLPASQTVAALFAGGATAEIWSVDGDINPHQRLRIETP
                     RDLNRMDPGERLVAISASPSPPRFEHIRKIVVQELQTEWIASRPEKMESYRLGTVVNP
                     VPPPPDWKRTIGQIPPWHRVVATEEMSHLPYLDHAVFCWADPQAEILDGSGDYLFRQS
                     LQPGRPEEIAQAILRVATNGKAIEIAFNQYPVHLAAGERSGIMTFEVTSLLTNGPNIL
                     ALRIREDPSSPEEQYGVAFHLEVTRRPAGRAAPPRPADGALLLTEEGDRLWGRLAGLR
                     GNTALLNSPFGELRLPLESTIGLLFPLGWRPDAPAPGLIDRLPLFGGAADTAPTIQGR
                     WGLPIDTRPDPVQDLMLLTEGRVSPAQPSYAVGDTLHVEGFEGRQVTMPISDVLGIYP
                     PRTISWTFKRPDEQAAVLYCELTTTGNDQVGGLLRKLDGRELMLEAFDGQFLRVRSEW
                     ISELFFPYHSGRRPGGTRNQSIAIINQFSGQEAFRDAFLQDAHRVQEAIFSIRADSVE
                     ISADVLASTALLTPAQYPVLVSVDPLGEYLHTLTESGDAVDALLQYLDRGGVLIALSR
                     GGAFRSAVRRGSTGLTRTPTELVQPSLSEQLGIRSVRPTDADAGGVISFAHPPNGLQP
                     IHFQRTGQLMEGLEGLPRHVTLAPMMSAPYFPMVGIEGQARVIYELVEEGGAIYGPAL
                     SVVPRGQGFLVLIDHLLWESRPDGRPFEKTILPILLQWALDTAGG"
     gene            complement(2724..4274)
                     /locus_tag="PLS90_14645"
     CDS             complement(2724..4274)
                     /locus_tag="PLS90_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPK03684.1"
                     /translation="MRCDRAQQLYDEYSRGSLPPATMARLEEHLAACPPCRETFELND
                     QIARLMRRATDVAHPGPQYFDDLAARVLDRLDAGERPVGPLDGGRGAAPWRRPLWWSG
                     FSAAAALLALAVFPPPDAARVSRVAESQIAAPGPGTVVVADGARRELVLTSPAAPFAA
                     SEISSASPGRMLDKGLEGRNSNTARVEQLISPSGPSLSPSARSGSGSAELDVEPHIEV
                     VKLQEPADPRTRIPTEVIEQLQLIESQMAVGGEDQLRVHLRELDRMVQARVSADSILR
                     DMPGVRQARLYLQADEQLADGHADEAMTAFTQVVVIDERAPLALRAALQIADLAYGER
                     ANFGQAREYYARCTGPEAARALTAAERRHVEGQLERLRRFSENNWQDLEMLHLVQRGE
                     WSQVVPALGNLIERPPAAPLLPEAARSIAARLEHARLQSDSDIVQQVYNLLAKRVMVE
                     QNEDIRAWLDLALGDILLTHYQDYQQALNRYQRVLSGSSTSGAATLARAKCDQMIERR
                     LNDDMARY"
     gene            complement(4441..5061)
                     /locus_tag="PLS90_14650"
     CDS             complement(4441..5061)
                     /locus_tag="PLS90_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK03685.1"
                     /translation="MALADIPAELILSARRGDRARTEQLLRLVAPDVYRIAYSMLRDH
                     EDTDEVVQESLIRMFRYIGNLKDPERFASWVMRITVNQVQTWRMRKNRRRLYEMDEGL
                     EPEQGAIVLSGTQGGNPREEAIRREIRREIEQAMAGLPDRQQMAVMLFEIEGCSIKEI
                     AEAMACSEGAVKFNIHEARKKLQRKLGHLVHGLRQRAARRRAAGDS"
BASE COUNT          874 a         1636 c         1913 g          834 t
ORIGIN      
        1 gctctttcat cggcgccgtg gaacaccgcc gagctgatcg actaccaggc gggcgcgatc
       61 gtcagccgcc agatcctcaa gcagaagacg gggtcggtga cgctgttcgc gttcgatgcc
      121 ggcgagagcc tgagcgagca tacgacgtcg ttcgatgccg tggcgctggt gctggacggg
      181 cgggcgcgga tcacgatcgg cggcgcgccg cacgtggtgg cgaccggcca gagcatcctg
      241 atgccggcgg gcattccgca tgccgttgcc gccgacgagc ggttcaagat gctgttgatg
      301 atgctgcgcg gcgagtaaca gatgggatca cgaaaggcac cagaggacac gcaaaaagac
      361 aaaaaaaatt gtccacgttc gcgagccgaa gacgaaggac gattttagat tgagttgctc
      421 ataaaccgtc gtcatggcga agaacggaat ggagggcagg gccactcagg agcgtggcgc
      481 tcccgggttt ctcagccgcc ggcggtgtcg agagcccact gcaacaggat cggcaggatc
      541 gtcttctcga acgggcggcc gtcgggacgg ctttcccata gcaggtgatc gatcagcacg
      601 aggaagccct ggccgcgcgg aacgaccgac agggccggtc cgtagatggc gccgccctcc
      661 tcgacgagct cgtagatcac gcgcgcctgg ccctcgatgc cgaccatcgg gaaatagggg
      721 gccgacatca tcggcgccag cgtgacgtgg cgggggaggc cttcgagtcc ctccatcaac
      781 tggccggtgc gctggaaatg aatcggctgc aggccattgg gtgggtgggc gaaggagatg
      841 acgccgccgg catcggcatc ggtcggccgg accgagcgga tgccgagctg ttcgctgagc
      901 gaaggctgga ccagctcggt cggtgtgcgg gtcaagccgg tcgatccccg gcggacggcg
      961 gagcgaaacg cgccgccgcg cgacagggca atcagcaccc cgccgcggtc gaggtattgc
     1021 agcagggcgt cgaccgcgtc gccgctctcg gtcagcgtat gcaggtattc gcccagcgga
     1081 tcgactgaaa ccaggacggg gtattgcgcg ggggtcagca gcgcagtgga ggcgagcacg
     1141 tcggccgata tttcaaccga atcggcgcgg atggagaaga tcgcctcctg gacgcggtgg
     1201 gcgtcctgga ggaaggcatc gcgaaaggct tcctggccgc tgaactgatt gatgatcgcg
     1261 atcgactggt tgcgcgttcc gccggggcgt cggccgctgt gataggggaa gaacagctcg
     1321 ctgatccatt cggaccggac gcgcaggaac tgaccgtcga aggcctcgag catcagctcg
     1381 cggccgtcca gcttgcgcag caggccgccg acctggtcgt tgccggttgt cgtcagctcg
     1441 cagtaaagaa cggcggcctg ttcgtcgggg cgcttgaacg tccaggagat cgtgcgcggc
     1501 ggatagatcc ccagcacgtc gctgatcggc atggtgacct gccggccttc gaagccttcc
     1561 acatgcaggg tgtcgccgac cgcataagag ggttgggcgg ggctgacgcg gccttcggtc
     1621 aagagcatca ggtcctggac ggggtcgggg cgcgtgtcga tgggcaggcc ccagcggccc
     1681 tggatcgtgg gggcggtgtc ggcggcgccg ccgaagagcg gaagccggtc gatgaggccg
     1741 ggagcgggcg cgtcgggccg ccagcccagc gggaagagca gaccgatcgt cgattcgagg
     1801 ggcaggcgca gttcgccgaa gggggagttg agcagtgccg tgttgccgcg caggccggcc
     1861 aggcggcccc acagccgatc gccctcctcg gtcagcagca gcgcgccgtc ggcggggcgc
     1921 gggggcgcgg cgcggccggc cggccggcgg gttacttcca agtgaaacgc gacgccgtat
     1981 tgttcctccg gcgatgaggg gtcctcgcgg atgcgcagcg cgaggatatt ggggccgttg
     2041 gtcagcaggc tggtcacttc gaacgtcatg atgcccgagc gctcgccggc ggccaggtgg
     2101 accggatact gattgaaggc gatttcgatc gccttgccat tggtcgcgac gcgcaggatg
     2161 gcctgggcga tctcttccgg ccggcccggc tgcagactct ggcggaagag ataatcgccc
     2221 gatccgtcga ggatttcggc ctgcgggtcg gcccaacaga aaaccgcgtg atccaggtag
     2281 ggcaggtggc tcatttcctc ggtggcgacg acgcgatgcc acggcgggat ctggccgatc
     2341 gtccgcttcc agtcgggcgg aggggggacg gggttgacga ccgtgcccag gcggtaggat
     2401 tccatcttct ccggccgact ggcgatccac tcggtctgga gttcctggac aacgattttg
     2461 cgaatgtgct cgaagcgcgg gggcgagggg gaggcggaga tcgccaccag gcgctcgccg
     2521 gggtccatcc ggttcagatc gcggggggtc tcgatgcgca ggcgctggtg ggggttgatg
     2581 tcgccgtcga cggaccagat ttcggccgtc gcgccgccgg cgaagagtgc ggcgaccgtc
     2641 tgcgacgccg gcaaccatgc cagcgccagc cccatgagca gacggatggg cgatggccgt
     2701 cgtcggatgg agcgaggcaa agtctaatac cgagccatgt catcgttgag tcggcgctcg
     2761 atcatctgat cgcatttggc ccgggccagg gtggcggccc cggaagtgga ggaaccgctc
     2821 aacacgcgct ggtagcgatt gagcgcctgc tgataatcct gataatgggt caacaggata
     2881 tcgcccaagg ccagatcgag ccaagcccga atgtcctcgt tttgctccac catgacccgc
     2941 tttgcaagca aattgtaaac ctgctggacg atgtcggaat ctgactggag gcgcgcgtgc
     3001 tccaggcgcg cggcgatcga gcgggccgcc tcgggcagca gcggggcggc cggcggtcgt
     3061 tcgatcaggt tgcccagcgc cggcaccacc tgggaccatt cgccgcgctg gaccagatgg
     3121 agcatttcca gatcctgcca gttgttctcg gagaaccggc gcaggcgctc cagttgtccc
     3181 tcgacatggc gccgttccgc ggcggtcagg gcgcgggcgg cttccgggcc ggtgcagcgg
     3241 gcatagtatt cgcgcgcctg gccgaagttg gcccgctcgc cataggccag gtcggcgatc
     3301 tggagggcgg cgcgcagcgc cagcggcgcc cgctcatcga tcacgacaac ctgcgtgaag
     3361 gcggtcatcg cctcgtcggc gtggccgtcg gcgagttgct cgtcggcctg caggtagagc
     3421 cgcgcctggc ggacgcccgg catgtcgcgc aggatgctgt cggccgagac gcgcgcctgg
     3481 accatccggt ccagctcgcg taggtggacg cgcaactgat cctcgccgcc gacggccatc
     3541 tggctttcga tcaactgaag ctgctcgatg acctcggtcg ggatgcgcgt gcgcgggtcg
     3601 gccggttcct gcagcttgac gacttcgatg tggggttcaa cgtcgagctc cgccgagccg
     3661 gagccggacc gggccgaggg cgagagggaa ggcccggacg ggctgatcag ttgttccacc
     3721 cgggcggtgt tggagttacg cccctcgagg cccttgtcga gcatgcggcc gggcgaggcg
     3781 ctggagattt cgctggcggc gaagggggcg gccggcgagg tcaggacgag ctcgcggcgc
     3841 gcgccatcgg ccacgacgac cgtaccggga cctggggcgg cgatctggct ttccgccacg
     3901 cgcgagacgc gggcggcatc aggcgggggg aagacggcca gcgccaacag agccgccgcg
     3961 gcggaaaacc cgctccacca cagcggccgg cgccatgggg cagcgccgcg cccgccgtca
     4021 agcggaccga cgggccgttc ccccgcgtcc aggcgatcca ggacgcgcgc ggcgaggtca
     4081 tcaaagtatt gcgggccggg atgcgccacg tcggtggcgc ggcgcatcag ccgggcgatc
     4141 tgatcgttca gttcgaaggt ttcgcgacag ggggggcagg cggccagatg ctcctcgagc
     4201 cgggccatcg tggccggcgg caggctgccg cgcgaatact cgtcgtagag ctgctgcgcc
     4261 cgatcgcaac gcatgctcgt cctttcccca cggccgaccg ttgtcggccg cgccgaatcc
     4321 gtccaaccag acccaccgac cgccccgcgg gtttggcatc atttagggtc ttggggagaa
     4381 ggtgagtgca ggcgagaaac agggattttc gagggacgag gaacgaattt cctggggggc
     4441 tcaggaatcg ccggcggcgc gccggcgggc ggcgcgctgg cgcagcccgt ggaccaggtg
     4501 ccccaacttg cgctggagct tcttgcgggc ctcgtggatg ttgaacttga cggccccttc
     4561 gctgcacgcc atcgcctcgg cgatttcctt gatcgagcat ccctcgatct caaatagcat
     4621 caccgccatc tgctggcggt cgggcaggcc ggccatcgcc tgctcgattt cgcggcggat
     4681 ttcgcgccgg atggcttcct cgcgcggatt gcccccctgg gtgccgctca ggacgattgc
     4741 cccctgctcc ggctcgagcc cctcgtccat ttcatacaac cggcggcgat tcttgcgcat
     4801 gcgccacgtc tgcacctggt tgacggtgat ccgcatcacc caggacgcaa accgctccgg
     4861 atccttcaga ttcccaatat agcggaacat ccggatcagg ctctcctgga cgacctcgtc
     4921 ggtgtcctca tggtcgcgca gcatggaata ggcaatgcgg tagacgtcgg gggcgaccag
     4981 acgcagcaac tgctcggtcc gcgcgcggtc gccgcggcgg gcggagagga tcagctcggc
     5041 ggggatgtcg gcaagggcca tcggggggcc aatcggtcgg cggcgggggc cgccggatct
     5101 gggaagacgg gacgatcacc cgcctgccaa cagacgtcgg cttgtcgaac gctactacat
     5161 aacgcggttg ggcgtcggaa tcaagcccga attgctaaga catgaacaaa aattttggcc
     5221 ccggcggagg cggcgcctcc cgccggaccc gcgaaaa
//
LOCUS       DAOPCO010000277         5251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13392_length_5251_cov_4.115858, whole genome shotgun sequence.
ACCESSION   DAOPCO010000277 DAOPCO010000000
VERSION     DAOPCO010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13392_length_5251_cov_4.115858"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLS90_14655"
     CDS             complement(<1..219)
                     /locus_tag="PLS90_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03686.1"
                     /translation="MRLLVRPLLLVCLLGPALRANAEGWLIDLSVKGAADAAKAAGLQ
                     PARTYRHFSWAYFRGAEEAEARFTQKARA"
     gene            complement(353..1117)
                     /locus_tag="PLS90_14660"
     CDS             complement(353..1117)
                     /locus_tag="PLS90_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPK03687.1"
                     /translation="MLEIFLAPENLIALLTLVALELVLGIDNIVLIAIVAGRVPADRQ
                     NFTRRTGLALAMVMRIALLFSISWVMRLTAPVFTVAEQSVSGKDLILLLGGLFLMGKA
                     TFEVHRNLEGEEEHAAGPGKGAGASIGGALATIMAMDLIFSLDSVITAVGMVDHISIM
                     VVAVVLSVGLMMLFAGPVSRFIHHHPTIKMLALSFMLLIGVMLLLEGLHHHIDKKYIY
                     FAMGFSLVVEMLNLKLLKPLKTGAVGAPGAPPPPAA"
     gene            1522..1989
                     /locus_tag="PLS90_14665"
     CDS             1522..1989
                     /locus_tag="PLS90_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03688.1"
                     /translation="MAKKNEEPQNDWDQIEIGDQVKHPKWGVGTVLFRSGMGDQAKAI
                     VVFPEEGQKKLMLKYAKVKKVGSTSLKSVEKLRAEAAEPKKAKKARPVRAKATDLAGL
                     PPDDEAEVGLDLEEVEDLGDEDLKLEDEEEVPGFDEDDEADFQGASAGDEDEL"
     gene            1986..2282
                     /gene="gatC"
                     /locus_tag="PLS90_14670"
     CDS             1986..2282
                     /gene="gatC"
                     /locus_tag="PLS90_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPK03689.1"
                     /translation="MSDFTRDEIHRLALLARLEFTDEQADAFAQQLTQILHYVETLNG
                     LDTTGVEPTSHALALHSVMRDDEPRPSLSNAEALANAPEQEAGCFRVPPIIQEL"
     gene            2327..3793
                     /gene="gatA"
                     /locus_tag="PLS90_14675"
     CDS             2327..3793
                     /gene="gatA"
                     /locus_tag="PLS90_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPK03690.1"
                     /translation="MTDAPAYATLKELQRSLEAGECSSRELTQALLDRIEALDGKLGA
                     FLRVTPDEALQQADAADAARRQGRRSPLLGIPLGLKDILCTRGVATTCASRILRDFIP
                     PYDATAVARLRQAGAVFLGKLNMDEFAMGSSCEFSAWQPTRNPWDPTRIPGGSSGGSA
                     AAVAAGFCPATLGTDTGGSIRQPAALCGVVGLKPTYGRVSRYGLIAFASSLDQIGPFA
                     RTVEDCALLLNAIAAQDPLDSTSAPVATSDYTECLGREIRGLRVGLPREYFIDGMEPA
                     VEAAVRRAVADLAAQGAEIVEISLPHTEYAVAVYYICATAEASSNLARYDGAHYGYRA
                     AGTRNIIEMYSRSRTEGFGEEVKRRIMLGTYVLSAGFYDAYYSKALKVRTLITRDFAE
                     AFRQVDVIATPTTPGVAFRIGEKLDDPLQMYLSDIFTISANLAGIPGLSLPCGFTAEG
                     LPIGLQLLAPAFEEARLLQVAHAYEQSHDWWRRRPELA"
     gene            3993..4502
                     /locus_tag="PLS90_14680"
     CDS             3993..4502
                     /locus_tag="PLS90_14680"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HPK03691.1"
                     /translation="MRTIVLLCVAALLTGCPEEDPKMQPVEDHPPTETPRDASTAPAT
                     NDAANADATDPARTPIPIDPVTADQIEIREDQKIAYLLDQIERSGLTFIAGDLEYDAA
                     GMAQLARERLSQQEERAWAAREFVDEIGGRSPDGHDFQVRFPDGRTLSAWDWLNGQLQ
                     DLMMQHRPQ"
     gene            4532..>5251
                     /gene="gatB"
                     /locus_tag="PLS90_14685"
     CDS             4532..>5251
                     /gene="gatB"
                     /locus_tag="PLS90_14685"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPK03692.1"
                     /translation="MTAFETVIGFEIHTELLTRSKVWCGCSTQFGQPPNTQVCPICLG
                     LPGSLPVLNRRALELALRACVALNCRIAPVIQFERKNYYYPDLPKNYQISQLRKNLGE
                     AGWLEFQVGDETRRVRINNVHLEEDAGKNIHPETPGADYSLVDLNRAGTPLLEIVTEP
                     DLRSAAEAAAFMNAMKSLLEYLEVSDCNMHEGRLRFELNISQRRAGEERLGTKVEVKN
                     LNSMKTVLRCIDAEEDRQRDLL"
BASE COUNT          953 a         1798 c         1696 g          804 t
ORIGIN      
        1 ggctcgggcc ttctgggtga accgcgcctc cgcctcctcc gcgccgcgga aatacgccca
       61 ggagaagtgg cggtaggtgc gcgccggctg aaggcccgcg gctttggcgg cgtcggcggc
      121 ccccttgacc gacaggtcga tcagccagcc ctcggcgttc gcgcggagcg ccgggccgag
      181 caggcagacg agcagcagag ggcggaccag cagacgcacc caaaggcggg atccagattg
      241 aaccattatc actccatgat ggccgaacgg ccagcgggcc tgccgggagg gccgctgtga
      301 gtataggagt tcccgctcat tttgtcacgt gagcgatgcg ccggcgcccc gctcacgcgg
      361 cgggcggggg cggggcgccg ggcgccccca ccgccccggt cttgagcggc ttgagcagtt
      421 tcagattgag catttcgacc acgagtgaaa aacccatcgc gaagtatatg tatttcttgt
      481 cgatgtgatg gtgcagcccc tccagcagca gcatcacgcc gatcagcagc atgaagctca
      541 acgccagcat cttgatcgtc gggtgatgat ggatgaagcg cgagaccggc ccggcgaaca
      601 gcatcatcaa ccccaccgac agcaccacgg ccacgaccat gatgctgatg tgatccacca
      661 tcccgacggc agtgatcacc gaatcgaggg aaaagatcag gtccatggcc ataatggtcg
      721 ccagcgcgcc gccgatcgag gcgccggccc ccttgcccgg ccccgccgcg tgctcctcct
      781 ccccctccag gttgcggtgg acctcgaacg tggccttgcc catcaggaac aggcccccga
      841 gcagcaggat cagatccttg ccggagaccg actgttcggc cacggtaaag accggcgcgg
      901 tcaggcgcat gacccaactg atcgagaaca gcagcgcgat gcgcatgacc atcgccagcg
      961 ccaggcccgt gcggcgggtg aaattctggc ggtcggccgg gacgcggccg gccacgatcg
     1021 cgatcagcac aatattgtcg atacccagga ccagctcaag cgcgacgagc gtgagcagcg
     1081 cgatcaggtt ttccggcgcg aggaagatct caagcatggc gatttttcgc tgtcgcgagg
     1141 attcagcccg ggctgtgcag cccgggagcg cccccggcaa gcgggggacg gcgggatccg
     1201 gcgcgccgcg cgcgccgtct catgcgttcg actcgctcac cttagcccaa ttcgacggac
     1261 gcccgaaagc ggtttgaacc aggatcgggc cgccgcctcc agctggcggg gcaggggagg
     1321 agctgcccat gaccgggggg gcaccccaga gacaataaat gcggggccat catcgttcgg
     1381 cttgccgctc agttttcagt tgaattccac ttggcgattg cccgaaggta attggtttgc
     1441 cggtccgccc gcctgccgcg gatccgctca gtctccgacc ggccgggcag ttccggagtg
     1501 gtcggccaga acgtctcaac catggccaaa aagaacgaag agccccagaa cgattgggat
     1561 cagatcgaga tcggcgatca ggtcaaacat ccgaaatggg gcgtcgggac cgtgctcttc
     1621 cggtcgggga tgggggacca ggccaaggcg atcgtcgtct ttccggaaga ggggcagaag
     1681 aagctgatgt tgaagtacgc caaggtgaag aaggttggtt caacatcact taagtcggtc
     1741 gagaagctgc gcgccgaggc cgccgagccc aagaaggcga aaaaggcccg ccccgtccgc
     1801 gccaaagcga ctgatctggc cggcctgccc ccggacgatg aggccgaagt cgggctcgac
     1861 ctggaggaag tcgaagacct cggcgacgag gatctcaagc tggaggatga ggaggaagtc
     1921 cccggcttcg acgaggatga tgaagcggac ttccagggcg cgagcgcggg cgacgaggac
     1981 gagctgtgag cgacttcacg cgcgatgaga tccaccgcct ggcgctcctc gcgcgcctgg
     2041 agttcaccga cgagcaggcc gacgccttcg cccagcaact gacgcagatc ctccattatg
     2101 tcgaaacgct caacgggctc gatacaactg gcgtcgagcc cacgtcgcat gccctggcgc
     2161 tccacagcgt catgcgcgac gacgaaccgc ggccttcgct ctccaacgcc gaggcgctgg
     2221 ccaacgcccc cgaacaggaa gccggctgct tccgcgtgcc gccgatcatc caggagctct
     2281 gaaccttccg gtccctcgcg gcggcaacgg accgggctgg atcctcatga ccgacgctcc
     2341 cgcctacgcc accctgaagg aactccaacg ctcgctcgaa gcgggcgaat gctccagccg
     2401 tgagttgacc caggccctgc tcgaccggat cgaggcgctc gacggcaagc tcggcgcctt
     2461 cctgcgcgtc acccccgacg aagcgctcca gcaggccgat gcggccgacg cggcgcgccg
     2521 ccaggggcgc cgctcgccgc tgctcggcat tcccctcggc ctgaaggaca tcctctgcac
     2581 gcggggggtc gcgacgacct gcgcctcgcg gatcctgcgc gatttcatcc ccccctacga
     2641 cgcaacggcc gtggcgcggc tgcggcaggc cggcgcggtt ttcctcggca agctcaacat
     2701 ggacgagttt gcgatgggct ccagttgcga gttctccgcc tggcagccga cgcgcaaccc
     2761 ctgggacccg acgcgcatcc ccggcggcag ctcgggcggc tcggcagcgg ccgtcgccgc
     2821 cggcttctgc cccgccacgc tcggcaccga caccggcggc tcgatccgcc agccggcggc
     2881 cctttgcggc gtcgtcggcc tgaagccgac ctacggccgc gtctcgcgct acggcctgat
     2941 cgccttcgcc tcatcgctcg accagatcgg cccgttcgcc cgcaccgtcg aggattgcgc
     3001 gctgctgctc aacgcgatcg ccgcgcagga cccgctcgat tccacctccg cgccggtggc
     3061 gacgtctgac tataccgaat gcctgggccg cgagattcgc ggtctgcgcg tcggcctgcc
     3121 gcgcgaatac ttcatcgacg ggatggagcc ggcggtcgag gcggccgtgc gccgggccgt
     3181 cgcggatctg gccgctcagg gcgccgaaat cgtcgagatc agtctgccgc acaccgaata
     3241 tgccgtcgcc gtctattaca tctgcgccac ggccgaggcg tcgagcaacc tggcccgcta
     3301 tgatggggcg cactacggct accgggccgc cgggacccgg aacatcatcg agatgtacag
     3361 ccgctcgcgg accgaagggt tcggcgagga ggtgaagcgg cggatcatgc tggggaccta
     3421 cgtcctgtcg gcggggttct acgacgccta ctacagcaag gcgctcaagg tgcgcacgct
     3481 gatcacccgc gacttcgcgg aagcgttccg ccaggtcgac gtgatcgcca cgccgaccac
     3541 gccgggcgtg gccttccgca tcggcgagaa gctcgacgac ccgctgcaga tgtacctgag
     3601 cgacatcttc acgatctcgg ccaatctggc cggcatcccc ggcctgtcgc tcccctgcgg
     3661 gttcacggcc gaaggcctgc cgatcggctt gcagctcctg gcacccgcct ttgaggaggc
     3721 gcgcctgttg caggtcgctc acgcctacga gcaatcgcac gactggtggc gccgccgccc
     3781 cgaactggcc tgagccggga gagcttcatc tttggcggtc gccggcgtca ccctgaaaga
     3841 ctgaaccgcc agcttgatgt ccgttacggc tcttatgtcc cttatgcccc tgtagaatcc
     3901 tgggtcgaac aacccggtgg ccgggatcaa cgcgaacgcg gcggaactca atcaccccga
     3961 ttggagcttt cgaaaagcaa ggagccggaa tcatgcgcac gatcgttctg ctgtgtgtcg
     4021 ccgctctgtt gaccggttgt ccggaggagg acccgaagat gcaacccgtg gaagaccatc
     4081 cgccgacgga aaccccccgc gacgcctcca cggccccggc gacgaacgac gccgcgaacg
     4141 cggacgctac ggatcccgcc cgcacgccga tcccaatcga cccggtcacg gccgatcaga
     4201 tcgagatccg cgaggaccag aaaatcgcct acctgctcga tcagatcgag cgctccggcc
     4261 tgaccttcat cgccggggac ttggaatacg acgcggccgg gatggctcag ctcgcgcgcg
     4321 agcggctctc gcaacaggag gagcgcgcct gggcggcgcg cgagttcgtc gatgagatcg
     4381 gcggccgctc gcccgacggc cacgatttcc aggtccgctt ccccgacggc cggacgctct
     4441 ccgcctggga ctggttgaac gggcagttgc aggacctcat gatgcagcac cgtcctcaat
     4501 gacggtcgac cacgagcggg agcgatgatc gatgacggcg tttgagacgg tgatcggttt
     4561 tgaaatccac acggagctct tgacgcgctc gaaggtctgg tgcggctgct cgacccagtt
     4621 tggccagccg cccaacacgc aggtctgccc gatctgcctg ggtctgcccg gctcgctgcc
     4681 ggtgctcaac cgccgcgccc tggaactggc gctgcgcgcc tgcgtggcgc tcaactgccg
     4741 gatcgccccc gtcattcagt tcgaacgcaa aaactactat taccccgacc tgcccaagaa
     4801 ctaccagatc agccaactac ggaagaacct gggcgaggcc ggctggctcg agttccaggt
     4861 cggtgacgag acccggcgcg tccggatcaa caacgttcac ctcgaggaag acgccggcaa
     4921 gaacatccat cccgagaccc ccggcgccga ttactcgctg gtcgacctga accgcgccgg
     4981 cacgccgttg ctggagatcg tgaccgaacc ggacctgcgc tcggcggccg aggcggcggc
     5041 gttcatgaat gcgatgaaga gcctgctgga gtacctcgaa gtcagcgact gcaacatgca
     5101 cgaggggcgc ctccgcttcg agctgaacat ttcgcagcgg cgggcgggcg aggagcgcct
     5161 cggcacgaag gtcgaggtca aaaacctcaa ttccatgaaa acggttctgc gctgcatcga
     5221 cgccgaggag gatcgccagc gcgatctgct c
//
LOCUS       DAOPCO010000278         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13468_length_5229_cov_4.222072, whole genome shotgun sequence.
ACCESSION   DAOPCO010000278 DAOPCO010000000
VERSION     DAOPCO010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13468_length_5229_cov_4.222072"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="PLS90_14690"
     CDS             complement(<1..1644)
                     /locus_tag="PLS90_14690"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HPK03693.1"
                     /translation="MGNAVRFRLLLILLLGFSLPPVARAQGADVITQRFGEQATCDVT
                     GVTMDARVSSQDKNLIDGAAETHYYRWQNHILMRFDLSSIPQGARVRRAVLRFAYHQN
                     FHDGGKTGVVLPIRKVTDPNGTGLWNESSVSYSAKRPGVAWSAGGDLGSVLGETIGEV
                     YYREWFAGGQDYLEAELTAAAQDWVNNPAGNLGLFMPCHENFNKRIAAKEYPNAALRP
                     YLEVTWEGPNTEAIPQPTQLQARHYSGQTFITWKEAQTGKDETSYRIYRHSQPITAAN
                     LDQAELVDQVYQGSSWLTDSIFNPATFKQPDLTPAGVTLAADSGLYVLPVEQSGSSYY
                     AVTTVVEGNENRTLAGGVNATANPVAEQPGHPAAFRFNAEYWYVGYVMWLGRYNPNDP
                     SDAFGLDNRRSAPFFFSVVQPEQYDAAKKYPLTLYLHAYSKSYFGSTEGKDNALYLDA
                     TRYGGFCMGFNDIPTLVARKANGTLVDLGPYLETDQMGYTYYTGWNSNYQPQKLVEGF
                     RKSLTVARPFEQGKSVAYSEKAMIYVTEWMLKRSPWAGAM"
     gene            complement(2430..3482)
                     /locus_tag="PLS90_14695"
     CDS             complement(2430..3482)
                     /locus_tag="PLS90_14695"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPK03694.1"
                     /translation="MTRVALIFGGVSPEHEVSIHSAGKVAEALREIAPARGLSLVPIY
                     INREGEWVFWTGDWPSEATIGEAPKWDARPEDFPVQALPFHRALGQMVEQRIGVAMIM
                     IHGIGGEDGRIQAALDLAEIPYTGTGAAASALALDKPRCQAVLNAAGLPIANSACLTS
                     DGIDGAERLVEVVGLPCVIKPARGGSSVGVTIVRELEQLRPALEAAFAIDEELMAERL
                     VTGREFTVGLIERDGGLLVTPVTEIIPPEGRYFDYDAKYTVGVSREITPAEIPAQLAE
                     RLRSLAAAAFRAVGCRGFARVDMIADPAAPTILEINTIPGMTATSLLPQGAAAMGISF
                     SELLGFFLDSARFDRG"
     gene            complement(3487..4143)
                     /gene="rdgC"
                     /locus_tag="PLS90_14700"
     CDS             complement(3487..4143)
                     /gene="rdgC"
                     /locus_tag="PLS90_14700"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HPK03695.1"
                     /translation="MPLTHGTVSGRIYRVTDTLPNAFKEQFERNLARHAFKPIDPERG
                     QLEAIGWVNIRQMLDSRLTLNKVLFGNLILVGLRIDKLTINQKLFRATLMQEIGRVMK
                     EKDKRNLSKEERLVIEDKVRLELIKRTQPNTAVHEMAWHLEEGVVFFGTTGERMNMAF
                     SDLFTETFQVGIEPQYPYLRAQRWAEKQGLGQDLLELLPSPFSPDAPVEVVEIGSEAE
                     "
     gene            4476..>5229
                     /locus_tag="PLS90_14705"
     CDS             4476..>5229
                     /locus_tag="PLS90_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017937353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPK03696.1"
                     /translation="MGTAQGSVRWGIERRLEFIEFRLFWEGHVNRGDLMAAFGISINQ
                     ASTDLNRYLGMAPGNMVYDKSARAYVRGDRFEPLFLRPDPASYLSQLRSILDGIVTQE
                     ETWIGQLPAFDTVPSPVRGIDAGNLRTVLQAIHNRQAIEVEYQSLSSPAPRWRWIVPH
                     AIAFDGFRWHARAYCFTDRCFKDFLLARILGIRDSRPSDVDASQDADWHTMVTLEIGP
                     HPGLSEAQKKVVALDYGMRNGKCNVTVRKALLY"
BASE COUNT          934 a         1716 c         1619 g          960 t
ORIGIN      
        1 catcgcaccg gcccacgggc tgcgcttgag catccattcg gtcacgtaga tcatcgcctt
       61 ttcgctgtag gcgacgctct tgccctgctc gaacggccgc gcgacggtca ggctcttgcg
      121 gaatccttcg acgagtttct gcggctgata gttcgagttc cagcccgtgt aatacgtgta
      181 gcccatctga tcggtttcaa gatacggacc caggtcgacc agcgtgccgt tggccttgcg
      241 cgccaccagc gtcggaatgt cgttgaagcc catgcagaag ccgccgtagc gggtcgcgtc
      301 gaggtagagc gcgttgtcct tgccctcggt gctgccgaaa tacgacttgc tgtaggcgtg
      361 caggtagagg gtcagcgggt atttcttcgc ggcgtcgtat tgctcgggct gaaccaccga
      421 gaagaagaac ggcgcgctgc ggcggttgtc caggccgaag gcgtcgctcg gatcgttcgg
      481 gttataacgc cccagccaca tcacgtagcc gacataccag tattcggcgt tgaagcggaa
      541 ggcggcgggg tggccgggct gctcggcgac gggattggcc gttgcattga cgccgccggc
      601 gagggtccgg ttttcgttcc cttccacgac ggtcgtcacg gcgtagtagc tcgatccgga
      661 ctgctcgacg ggcaacacgt agagaccgct gtcggcggcc agcgtcacgc ccgcgggggt
      721 cagatccggc tgcttgaagg tggccggatt gaaaatcgaa tcggtgagcc aggaggaacc
      781 ctggtagacc tggtcgacca gttcggcctg gtccagattg gcggcggtga tcggctgtga
      841 gtggcgatag atccggtaac tcgtctcgtc cttgccggtc tgcgcttcct tccaggtgat
      901 gaacgtctgg ccggagtaat gacgggcctg aagctgcgtc ggctgaggaa tcgcttcggt
      961 gttgggccct tcccaagtca cttccaggta ggggcgcagc gcggcgttgg gatattcctt
     1021 cgccgcgatc cgtttgttga aattctcatg gcacggcatg aacaggccga ggttgccggc
     1081 ggggttgttg acccagtcct gcgcggcggc ggtcagctcg gcctccaggt aatcctggcc
     1141 gccggcgaac cattcgcggt agtagacctc gccgatcgtt tcgcccagca cgctgcccag
     1201 gtcgccgccg gccgaccagg cgacgccggg gcgcttggcg ctgtagctga cgctcgactc
     1261 gttccagagg ccggtgccgt tcgggtcggt caccttgcgg atcggcagca cgacgccggt
     1321 cttgccgccg tcgtggaaat tctgatgata ggcaaaacgc agcaccgcgc ggcggacgcg
     1381 cgccccctgc ggaatcgacg acaggtcgaa ccgcatcagg atgtggttct gccagcggta
     1441 gtagtgggtt tcggcggcgc cgtcgatcag gttcttatcc tgcgaactga cgcgcgcgtc
     1501 catggtgacg ccggtgacgt cgcaagtggc ctgttcgccg aagcgctggg taatgacatc
     1561 ggcgccctgc gcgcgcgcaa ccggcggcag ggagaatcca agcagcagga tcagcagcag
     1621 gcggaaacgc accgcgttgc ccatgacgcg cgaacgttcc gtggcggtat gacgtatttc
     1681 gactttcacg ttgcagtcga ctccatccct gagatcggca acggtggggg aggcgccggc
     1741 ggacacaagg cgctctcctg cgccgtgaca actgcccgcg gtgcccttcg cactcgctca
     1801 ctgctctcat cgtggaagtc gccgattgcc tgaggggaga atccgagggg ggactggaac
     1861 aaaaatcaaa cgcccccgtg tggaggcgtg tcgcattgtg aatatttgac ttaggcgttc
     1921 aatgcggcgc ggccgccgtt ggcgccggtg tgcccttttt agtacgacgc gggcggtcta
     1981 atttaacctg cttaagttca tcgaatgcgc gcgcggaggt tgaacgggtg aattgaatcc
     2041 gaccgatccg acagatccga cggattagga atccgccgca ccggcgctcc gttcccccgc
     2101 caccatccgg gaaggccggg gggtccggcc ccggcatccg gcgcgcgttg agcccgcgag
     2161 aagcccgcct ccgtatgatg gaacggtcgg agcgagagct ccttcaataa acaatcaacc
     2221 cttcgaccgc tgatgggtca gcggcatact ttaagcgtct ggaggaacat ggatgcacgt
     2281 agatccggca tggcgttcgt cacgactggt atcaaaaccc atgaaccggt gtatctgtca
     2341 gctcgcgaga tcaaggagca gcttgctcga cagattcagc ggaagcttcg ggattgacac
     2401 cgcggagagc tgccgcgcaa tcagtcgagc tatccccggt cgaaccgggc cgaatccagg
     2461 aaaaaaccca ataactcgga gaagctgatg cccatggccg ccgcgccctg agggagcagg
     2521 ctggtggcgg tcatgccggg gatcgtgttg atctccagga ttgtcggcgc cgcgggatcg
     2581 gcgatcatgt cgacgcgcgc gaagccccgg caacccaccg cccggaacgc cgccgccgcc
     2641 agcgaccgca gccgctcggc cagttgcgcc ggaatctcgg ccggcgtgat ctcgcgcgac
     2701 acccccaccg tatacttggc atcgtagtca aaatagcgcc cctcgggcgg gatgatttcg
     2761 gtcaccggcg tcaccagcaa tccgccatcg cgctcgatca gcccaacggt gaactcgcgc
     2821 cccgtcacca ggcgctcggc catcagttcc tcatcgatgg cgaaggccgc ctccagcgcc
     2881 ggccgcaact gctccagctc gcgcacgatc gtcacgccga cgctcgagcc gccgcgcgcc
     2941 ggcttgatca cgcacggcag gccgaccacc tcgaccagcc gctcggcgcc gtcgatgccg
     3001 tcgctcgtca ggcaggctga attcgcgatc ggcagccccg ccgcgttgag caccgcctgg
     3061 cagcgaggct tgtccagggc gagcgccgag gcggccgcgc cggtcccggt ataggggatc
     3121 tccgccaggt cgagcgccgc ctgaatccgg ccgtcctcac cgccgatgcc gtggatcatg
     3181 atcatcgcga cgccgatccg ctgctccacc atctgcccca gcgcgcgatg aaagggcagc
     3241 gcctggaccg ggaaatcctc cggccgcgcg tcccatttcg gcgcctcgcc gatcgtcgcc
     3301 tcactcggcc agtcgccggt ccagaagacc cactcccctt cccggttgat atagatcggg
     3361 acgagcgaca ggccgcgcgc cggcgcgatc tcgcgcaagg cctccgccac cttgccggcc
     3421 gagtggatcg agacctcgtg ctcgggcgac acgcccccga aaatcagcgc aactcgggtc
     3481 acggcttcac tccgcctcgc ttccgatttc caccacttcc accggcgcat cggggctgaa
     3541 cggcgacggc agcagttcca gcaggtcctg gcccaggccc tgcttctccg cccagcgttg
     3601 cgcccgcaga taagggtact gcggctcgat gccgacctgg aacgtttcgg tgaacagatc
     3661 ggaaaacgcc atgttcatcc gttcgcccgt cgtcccgaaa aagaccaccc cttcctccag
     3721 gtgccaagcc atctcgtgga ccgccgtgtt gggctgcgtg cgcttgatca gctccaggcg
     3781 gaccttgtcc tcgatcacga gccgctcctc cttggagaga ttgcgcttgt ccttttcctt
     3841 catgacgcgg ccgatctcct gcatcagcgt cgcgcggaac agcttctggt tgatcgtcag
     3901 cttgtcgatc cgcagcccca ccagaatcag attgccgaaa agcaccttgt tgagcgtcag
     3961 gcgcgaatca agcatctggc ggatattgac ccagccgatc gcctccagtt gcccgcgctc
     4021 cggatcaatc ggtttgaacg catggcgcgc cagattgcgc tcgaattgct ccttgaacgc
     4081 gttgggcagc gtgtcggtca cgcgataaat gcgcccggag acggtcccat gagtcagcgg
     4141 catcgaattg ctcctgaaaa actacagccc tcgttcgtca acacgctcgg cgccgatccg
     4201 ccaaacccga tgatcttcca accgccgcga tgaaaactca agcgtccatc cgatccagtc
     4261 tgccgctcgg gccggggcaa ggcctcattc ccatatcgag ccaactttct gcttgatgat
     4321 taactagtca gaatattatc tagattcaat agaacacaac aggccgtggc gtgttcgacg
     4381 tgacgtagtc ttctccttgt tgcccgatct ttacaaaccg accagaaact ggtcgaatac
     4441 aattctgatg cgggtcatga tactggggtg caagggtggg gacagcgcaa ggcagcgttc
     4501 ggtggggcat tgagcgccga ctcgagttca tcgagttccg gctcttctgg gaaggccatg
     4561 tcaaccgcgg cgacctgatg gccgccttcg ggatctcgat caatcaggca tccaccgacc
     4621 tcaaccgcta cctcggcatg gcgccgggca acatggtcta cgacaagagc gcccgcgcct
     4681 acgtccgcgg cgaccgcttc gagcccctgt tcctgagacc ggacccggcc agctacctgt
     4741 cccagttgcg gtcgatcctc gacggcatcg tcacgcagga ggaaacctgg attggccagc
     4801 tgccggcgtt cgacacggtc cccagccccg ttcgcggcat cgatgccggc aacctgcgca
     4861 ccgtgctcca ggccatccac aacaggcagg ccatcgaagt cgaataccag tcgctctcca
     4921 gtccggcccc gcgctggcgc tggatcgtcc cgcatgccat cgcctttgac ggtttccgct
     4981 ggcatgcccg ggcgtactgc ttcaccgacc gctgtttcaa ggacttcctg ctggcgcgca
     5041 tcctcggcat ccgggacagc cgccccagcg acgtggacgc cagccaggac gccgactggc
     5101 acacgatggt gacactggaa atcggcccgc acccgggact gtctgaagca cagaagaaag
     5161 tggtggcgct ggactacggc atgcgcaacg gcaagtgcaa cgtcaccgtc cgcaaggccc
     5221 tgctctact
//
LOCUS       DAOPCO010000279         5218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13500_length_5218_cov_5.217509, whole genome shotgun sequence.
ACCESSION   DAOPCO010000279 DAOPCO010000000
VERSION     DAOPCO010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13500_length_5218_cov_5.217509"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(564..3848)
                     /locus_tag="PLS90_14710"
     CDS             complement(564..3848)
                     /locus_tag="PLS90_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPK03697.1"
                     /translation="MTERPPGAPLLREINWQTSYRHDDGDLVELFYVRALACAATYDR
                     TTGFFTADALALAARGLERLIANRGRMRLIVGCTLGPEEVAAIREGYDRRAKIAEVLG
                     ALTLVPPDLKARNGLEALAWMIEHGRLDIRVALPVDPATRLPVVAPGIYHEKVGVITD
                     FDGNRLSFSGSINETQGGWVNNRESFHVHCSWLGEREAWHVRDEVNAFEALWEDRARS
                     VQTLDFPEAARARLLEFLPRDDRFVAPTPAPRIGEEDGQAPEQGRAHEEGALLPEELR
                     RLAWALIRNAARMPNGIRVGEVTSTVNPWPHQLRTFKRMIEQWPFRMLLCDEVGLGKT
                     ITAGLVLRQLWLAGKARRILLLVPKAVIRQWQNELYEKFNLNVPWYDGRKLIWRTAHW
                     RRGSSEQVVGRNEWHKQPLVLASSQLMRRRNRRRELLRCDPWDLVLLDEAHHARRKGA
                     GSAREQGPNLLLSLMQELKDRTQGLLLLTATPMQVAPVEVWDLVNLLGLPPEWDAPSF
                     VRYFELAAGNPDHEELCWLARMFQATEAQFGLLSEAELEMLLPGVSRMRRRKIVDALR
                     DVSGIPLRMLSAEHRRGALELLRKASPLRWRMARSTRNLLRRYFHEGLLDKPVPAREV
                     KDRKIQLSAAERELYEAVEAYISDTYNAAAPDRRNAVGFLMTIYRRRLASSFHALRMT
                     LTNRLGNLNGMDVEEDLDQDELLDDPPGTEEASALMQTAAAVEETDRIHRLLKRIAML
                     GDADTKARCLLQELHAAMADGYESAIIFTQYTDTLDYLKDYLSTHLPELTMATYSGRG
                     GELRESTGEWKSCSRDRVKRELRQKKIRLLLCTDAAGEGLNLQYCGVLVNYDLPWNPM
                     KVEQRIGRIDRIGQEREVIRIVNLAYEDTVEADIYFSLGNRINLFQGIVGKLQPILSR
                     LPEQFERVALARRDRREEVRQQMLAGVELERQAAEAGTFDIDVVAPEELVAPEFPEPP
                     LTMDQLDKALNLEALRPADWEWRPLDHRSYSLRAPGMAEPIRVTTSPEVFDEHFESHV
                     FLSPGSEILGEVDEVNDIRTLSTKGFTVEVEPDGGWRIWAEQEGARQEVNSLQDLLAC
                     ADAVSG"
     gene            complement(3845..>5218)
                     /locus_tag="PLS90_14715"
     CDS             complement(3845..>5218)
                     /locus_tag="PLS90_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPK03698.1"
                     /translation="ADIRLGSADALDLADHSIDCIVMDPPYYDNVMYAELADFFYVWL
                     KRTAGLLWPEAFSDYLTDKDREAVANPAKFKGQKGAKKRAGVDYQQRMARIFAECRRV
                     LKPDGIMTLMFTHKATGAWDALASGLVEAGFVITASWPVNTEAEGSLHIKEKSAARST
                     ILLVCRPRAEAAESEDDRYWEDVEPLVRERVRQRVGEFQAAGIGGIDLYLASFGPALQ
                     VFSEAWPLKRGTPLPKPKEWEGDLFPDWDPYSARPEDALQAARAEVKRWRFEQLTTVK
                     RQHHLDALSEWFIMAWDAFGAPRFPADEALKLARVVGLDFDRAVKSVLCDVKGDDVLL
                     WDSQTRHAKRKLGPASDEVMIDTLHQAAWVARSQNTQAAVEMLKRGGFLQDATMKTAL
                     EALLNVLPAVKAGRGNGDDPLNAAASDFEALNNIRKLGFSDVPGPKFEQTEALDREAR
                     VKEGLHE"
BASE COUNT          872 a         1858 c         1479 g         1009 t
ORIGIN      
        1 aggcccttga tctggtctag ttcggggggc aggacctcca agttcgtgaa gtagcgcttg
       61 agatcgtgct tcgaccagat gttattccaa taatcgagcg tcagtaagac ggcgtaggtc
      121 tccgtcggat ctgcggggtg cacttgaatc tctatcccgg aaatatagcc gatcggcatc
      181 acagattttc tggacacttg tctacatttc gtcccagcat ctcccttatc tgtgtccaca
      241 catttctgca ttccgtgagg acccagtgca tcagtctgtg ggctctatga agcccttaaa
      301 tccgtatcgt cagcagcttt cctggcctga tcagcgactg acacccaact ctctcttgcg
      361 gtacggcctt caaagcgcag catcccaggc aatcgggtta tgaaattatg atgcaataaa
      421 tgcatcactt tacgacacga ttcgagactt catcggcgac cccgcgaagc ccccgcagcc
      481 gttatataat ggacatggaa tcattatgcg aggggcgtct atacgttatg cctgaggtcc
      541 ggtggatctt cggcactcaa cccttagccg ctcacggcgt cggcgcaagc caggaggtcc
      601 tgaagcgagt tcacttcttg tcgcgccccc tcctgctcag cccagatccg ccagcctccg
      661 tcgggctcca cttccactgt gaagcctttc gtcgagagcg tccttatatc gttaacttca
      721 tcaacttcac ccaggatttc gcttcccggc gaaaggaaga catggctttc aaagtgctcg
      781 tcgaaaacct cggggctggt ggtcacacgg atcggttccg ccattcccgg cgctcgcaat
      841 gaataggacc ggtgatcgag cggccgccac tcccaatcgg ccggccgcag cgcctccaga
      901 ttgagcgcct tgtccagctg gtccattgtc aagggcggct cgggaaactc cggcgcgacc
      961 agttcctcag gcgccaccac atcaatgtcg aaggttccgg cctcggcggc ctgtcgttcc
     1021 agctcgacac ccgccagcat ctgttggcgc acctcttccc gccggtcgcg ccgggccagg
     1081 gcgacgcgct cgaactgctc gggaagtcgc gaaaggattg gctggagctt accgacgatt
     1141 ccctgaaaca gattgatgcg attcccaagc gagaagtaga tatccgcctc aacggtatct
     1201 tcataggcca gattgacgat ccgaatgacc tctcgttcct gcccgatccg atcgatccgg
     1261 ccgatgcgct gctcgacttt catcggattc cagggcaggt catagttcac cagcaccccg
     1321 caatactgca ggttcaagcc ttcgcctgcc gcatcggtgc agagcagcag gcgaatcttc
     1381 ttctggcgca gctcacgttt tacgcgatcc cgcgagcagc ttttccattc acctgtcgat
     1441 tcccgcaatt cccctccccg accggaataa gtggccatag tcagctccgg caggtgcgtc
     1501 gagaggtagt ccttcaggta gtccagcgtg tcggtatact gtgtaaagat gatggctgat
     1561 tcgtagccat ccgccatcgc cgcatgcagt tcctgcagca ggcatcgcgc cttggtgtcc
     1621 gcgtcgccca gcatcgcgat ccgcttcagc agacgatgga tcctgtctgt ttcctcgacc
     1681 gcggcggcgg tttgcatcag cgcagacgcc tcctcggtcc ctggcggatc atccagcaac
     1741 tcgtcctgat ccagatcctc ctccacatcc attccattga ggtttcccag ccggttggtc
     1801 agggtcatcc gcaaggcgtg aaatgagctg gccagccgcc gccgatagat cgtcatcaaa
     1861 aaaccgacgg cgttgcgccg gtcgggcgcc gcggcattgt aggtgtcgct gatgtaggcc
     1921 tccacggcct cgtacagctc ccgctcggcg gccgacagct ggatcttgcg atccttgacc
     1981 tcacgcgccg gcaccggctt gtcgagcaag ccttcgtgga aataccgtcg caacagattg
     2041 cgggtgctcc gcgccattcg ccagcgcaac gggctggcct tgcgcagcag ttccagcgcg
     2101 ccgcgccggt gttcggccga aagcatccgg aggggaattc cgctgacgtc acgcaaggca
     2161 tccacgatct tgcggcgtcg catccgactc acccccggca acaacatctc gagctctgcc
     2221 tcgctcagca gaccgaactg tgcctccgtc gcctggaaca tccgggccag ccagcacagt
     2281 tcctcgtgat ccgggtttcc tgccgccagc tcgaaatacc gcacgaaaga cggcgcgtcc
     2341 cactccggcg gcaggccgag caggttgacc agatcccaaa cctcgaccgg agccacctgc
     2401 atcggcgtcg ccgtcagcag cagcagcccc tgcgtccggt ccttcagctc ctgcataagc
     2461 gacaacagca ggttgggacc ttgctccctg gccgaacccg ctcccttgcg ccgcgcgtgg
     2521 tgcgcctcat cgagcagcac cagatcccat gggtcgcagc gcagcagttc cctccgccgg
     2581 ttgcggcgcc gcataagctg gctgctcgcc agcaccagcg gctgcttatg ccactcattg
     2641 cggccaacca cctgttcgga cgacccgcgt cgccagtggg ccgtccgcca gatcagcttg
     2701 cggccgtcat accacggcac gttcaggttg aatttctcat agagctcgtt ctgccattgc
     2761 cggataaccg ccttcggcac cagcagcaga atccgccgcg ccttcccggc cagccagagt
     2821 tggcgcaaca ccagaccggc cgtgatcgtc ttgcccagcc ccacctcatc gcacagcagc
     2881 atgcggaacg gccactgctc gatcatccgc ttgaaggtgc gcagttggtg cggccacgga
     2941 ttaaccgtcg acgtgacttc gcccacgcgg atcccgttgg gcatgcgcgc cgcattgcgg
     3001 atcagcgccc atgcgagccg gcgtagttcc tcgggcaaca gggcgccttc ctcgtgcgct
     3061 cgcccttgct ccggcgcctg tccgtcctct tcgccgatgc gaggcgccgg ggtcggggcc
     3121 acaaaccgat cgtcccgcgg caggaactcc agcagcctgg cccgcgccgc ctcgggaaag
     3181 tccagcgttt gcacgcttct ggcccggtct tcccacaacg cctcgaaggc gttgacctcg
     3241 tcccgcacgt gccaggcctc ccgctcgccc agccacgaac agtgcacatg gaagctctcg
     3301 cggttgttga cccagccgcc ttgcgtttcg ttgatgctgc cgctgaagct gagccggttc
     3361 ccgtcgaagt cggtgatcac ccccaccttt tcgtgataga tccccggcgc gacgaccggc
     3421 aggcgggtcg cgggatcgac cgggagggcg acgcggatgt cgagccggcc gtgttcgatc
     3481 atccaggcca gcgcctccag gccattgcgc gctttcaggt cgggcggcac cagcgtcagc
     3541 gcgcccagca cttcggcgat cttggcgcgc cggtcatagc cctcgcgaat cgccgccact
     3601 tcctccggac ccagtgtgca gccgacaatc agccgcatcc gcccgcggtt ggcgatcagc
     3661 cgctccagcc cccgcgccgc cagcgccaag gcgtccgccg tgaagaaacc ggtcgtccga
     3721 tcataggtgg ccgcacaggc cagcgcgcgg acatagaaca gctcgaccag atcgccatca
     3781 tcgtggcgat agctggtctg ccagttgatc tccctcagga ggggagctcc tggaggtcgc
     3841 tccgtcactc gtgaagccct tccttcaccc gtgcttctcg gtccagcgcc tcggtctgct
     3901 cgaattttgg tcccggcacg tcgctgaaac cgagcttgcg gatgttgttc agcgcttcga
     3961 aatcgctcgc cgcggcgttg agcgggtcgt cgccgttgcc gcgcccggcc ttcaccgccg
     4021 gcagcacgtt cagcagcgct tccagcgccg tcttcatcgt ggcgtcctgc aggaacccgc
     4081 cgcgcttgag catctccacg gccgcctgcg tgttctggct gcgggccacc catgccgcct
     4141 ggtgcagcgt gtcgatcatc acctcgtcgc tcgccggccc gagtttccgc ttcgcgtgcc
     4201 gggtctggct gtcccacagc agcacgtcat cccccttcac gtcgcacagg acgctcttta
     4261 ccgcccggtc aaagtccagc cccaccacgc gcgccagctt cagtgcttcg tcggccggga
     4321 aacgcggcgc cccgaaggcg tcccacgcca tgatgaacca ctcgctgagc gcgtccaggt
     4381 gatgctgccg tttcaccgtc gtcagctgct cgaaccgcca gcgcttcacc tcggcccgcg
     4441 ccgcctgcag ggcatcctct ggccgggcgg agtaagggtc ccagtccggg aacaggtccc
     4501 cttcccactc cttcggcttc ggcagcggcg tcccccgctt gagcggccac gcctcggaaa
     4561 agacctgaag cgccggaccg aagctcgcca ggtagaggtc gatcccgccg atccccgccg
     4621 cctggaactc gcccacccgc tgccggaccc gctcgcgcac gagcggctcc acatcttccc
     4681 agtagcggtc atcctcgctt tcggccgctt ccgcccgcgg ccggcagacc agcaggatcg
     4741 tgctgcgcgc cgccgacttc tccttgatgt gcaggctccc ctcggcctcg gtgttcaccg
     4801 gccagctcgc cgtgatcacg aaccccgcct cgaccagccc cgaggccagc gcgtcccacg
     4861 ctcccgtcgc cttgtgcgtg aacatcagtg tcatgatgcc gtctggcttc agcacgcgcc
     4921 ggcactcggc gaagatccgc gccatccgct gctggtagtc cacgccggcc cgcttcttcg
     4981 cccccttctg ccccttgaac ttggccgggt tggccaccgc ctcgcggtcc ttgtccgtca
     5041 ggtagtcgct gaacgcctcc ggccagagca gccccgccgt ccgcttgagc cacacgtaga
     5101 agaagtccgc cagttcggca tacatcacgt tgtcgtagta cggcgggtcc atcacgatgc
     5161 agtcgatgct gtgatcggcc aggtcgaggg catcggccga accgagccgg atgtcggc
//
LOCUS       DAOPCO010000280         5211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13517_length_5211_cov_4.984678, whole genome shotgun sequence.
ACCESSION   DAOPCO010000280 DAOPCO010000000
VERSION     DAOPCO010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13517_length_5211_cov_4.984678"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLS90_14720"
     CDS             <1..724
                     /locus_tag="PLS90_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HPK03699.1"
                     /translation="AELLDVIQRVAARGGKIIVPSFSVERTQEIVYYLNALTNRRAIP
                     PIPIYVDSPLSVNVTDIFRLHPECYDEEMRRIVLEGDPFGFHRLSYVKDVEESKRLNN
                     INGPCMIISASGMCEGGRILHHLRNSIAEPRNCILFVGYQAEGTLGRKIVERQPSVNI
                     FGEPHPLRAEVVTLNTMSGHADRHDLLNYARVVREHSPNLKQVFLVHGEPAAQRTLAE
                     TLRAELGLDVVIPALRDEFRLL"
     gene            complement(1023..1256)
                     /locus_tag="PLS90_14725"
     CDS             complement(1023..1256)
                     /locus_tag="PLS90_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPK03700.1"
                     /translation="MPIYEYQCDHCEHVMEVRESYSAETRHECPACGSGPMRKLLSAF
                     SVGSGRPAPACAGPTCDPAPSCGAGGCCPCLGD"
     gene            1546..2274
                     /gene="pyrH"
                     /locus_tag="PLS90_14730"
     CDS             1546..2274
                     /gene="pyrH"
                     /locus_tag="PLS90_14730"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPK03701.1"
                     /translation="MSGATDDASTPRYRRILLKISGEFLGGGAAFGIDPGVINRLAHE
                     IKELTALGVQVGVVIGAGNLVRGSAVPDMNRAAADYIGMVATMINALMMQGALERIGV
                     ATRVMSAIEMREVAEPYIRRRAIRHLDRGEVVIFGCGTGNPFFTTDTAAALRANEIGA
                     DILMKATKVDGVYDADPAKVPGAVKFDELSYDQVVQRNLRVMDTSAVSLCRDNGLPIL
                     VFNLDQPGNVMRAVRGEKIGTLVH"
     gene            2370..2927
                     /gene="frr"
                     /locus_tag="PLS90_14735"
     CDS             2370..2927
                     /gene="frr"
                     /locus_tag="PLS90_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPK03702.1"
                     /translation="MLEALLKEAREKMDKAVQVVDHDLSALRTGRATIHLLDGISVAM
                     YGQEQPINQLATVSTPDAQTIAIQPWDRSALGPIEKAILQANIGLTPNNDGKIIRLNV
                     PPLTEETRRDVVKRAHAIAEEGRIAIRNIRRHVNDTLKKSEKEAGVSEDEKKRLLDQV
                     QKLTDEHIKQVDEHMAAKEAEIMKV"
     gene            complement(3192..4847)
                     /locus_tag="PLS90_14740"
     CDS             complement(3192..4847)
                     /locus_tag="PLS90_14740"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPK03703.1"
                     /translation="MLNSDDPLPSPDPVRASRLLTPRRLLALATVYLLVTLTVLIMLR
                     TGYGILKEEVRTKARDLATLLSSQIDPAALAEIRDPSDTDKPAFRRILMQIRTVKDVY
                     PDVRYIYIIRPDSVDQPTSWRFVIDAQPHDVDLNQDGTIQPSERGVLPGEPYEEAAGQ
                     PAIALALKRPTATSGYFRDEWGTYLSGFAPIREESTGRPIAVLGIDITQQTVRAKFFA
                     GNVVAFLALIFLLGLVTVTIFALYGKAQALEIVGRLHERVGSQNDQLQEANRRLAATV
                     AELTDRDRRMGRELRQAQEVQQRFLPRDYPFQDRLRCASFYRASRLIGGDLYDLLRVD
                     DQTAIFMIADISGHGVSSALLTSALKMLIDRRSEALIRPVAGGSAALVSDRYHVDCLQ
                     AFMAEVNHSLAEILIPGQFITCLLGVIHLDQNRLLLANAGHPAPLWTHRPAAAEPMRV
                     APLELPSNLALGLVANWKFQVVAHPFKPGERLFLYTDGLVERRNGAGEEFGAERLEQA
                     VAATTALPVDRAVGQVLQALEEFADGEEPHDDMAILLIERTEA"
BASE COUNT          938 a         1733 c         1654 g          886 t
ORIGIN      
        1 ggccgagttg ctcgacgtga tccagcgcgt tgccgcccgc ggcggcaaga tcatcgtccc
       61 cagcttctcc gtcgagcgca cgcaggagat tgtctactac ctgaacgcgc tgaccaaccg
      121 gagggcgatt ccgccgattc cgatatatgt cgacagcccg ctgtcggtca acgtgaccga
      181 catcttccgg ctgcacccgg aatgctacga cgaggaaatg cgccggatcg tcctggaggg
      241 cgacccgttc gggtttcatc gcctgagcta cgtgaaggat gtcgaggaga gcaaacggct
      301 caacaatatc aacgggccgt gcatgatcat ctcggcctcg ggcatgtgcg agggggggcg
      361 gatcctgcat catctgcgca attcaatcgc cgaaccgcgc aactgcatcc tgttcgtcgg
      421 ttatcaggcc gaggggacgc tcgggcgcaa gatcgtcgag cgccagccga gcgtcaatat
      481 cttcggcgag ccgcatccgc tgcgcgccga ggtcgtcacg ctcaacacga tgagcggcca
      541 tgccgaccgt cacgacctgc tcaactatgc ccgcgtcgtg cgcgagcaca gccccaacct
      601 gaagcaggtg ttcctggtcc acggcgagcc ggcggcgcaa cggacgctgg ccgagacgct
      661 gcgcgccgaa ctggggctgg atgtcgtcat tccggcgctg cgcgatgaat ttcggctgct
      721 ttgatggagg aaatcgcatt tcggaaggga cgcaagggac atagggttct gccgtcccga
      781 cgggactcgg tcgctctggt gatcccggtt cccgccactg actgccgtcc ctgacgggac
      841 tgccctgctg ttagctccgg cgggcctgga tcatacgaat cggccgttca ccccccccat
      901 tttcatccct cgttgtgaat cgcaggttca tgagcaattc gtactgccga tgcctgcccc
      961 aaaggaaaac ggcccgtcgc cgaggcaacg ggccgttctc ctccagagga ttaatagctg
     1021 agttaatccc ccaaacaggg gcagcagccg ccggcgccac aggagggagc gggatcgcag
     1081 gttggacctg cgcaagccgg cgccggacgt ccgctgccca cgctgaatgc cgacaggagc
     1141 ttccgcatcg ggccgctccc gcaggctgga cattcatgcc gggtctcggc cgaataggat
     1201 tcgcgcacct ccatcacatg ctcacagtgg tcgcactgat actcataaat cggcatcgtc
     1261 tgcaatctcc gcgcgttgca caaacccctg ggggggccgc cggtgaagcc tgctccaccg
     1321 gccattctta ttttacgccc gccgccggtt ccgattcaac ctcgtagtgg tcccccggtt
     1381 caggatcaac gtcggatgat gcttcagacc cgtgtgtccg cggggcttgc ccggcgtcct
     1441 gccgtccggc gctgattttg atcttctctt tttcgcccgg tcctttacac tcggacagca
     1501 gaccgcgcac ccgcgaaccc ccgctcccgg acgaaaggca gacgcatgag cggcgcgacc
     1561 gacgacgctt cgacaccccg ttaccggcgg atcctgctca aaatcagcgg cgaattcctt
     1621 ggcggcggcg ccgcgttcgg gatcgatccg ggggtgatca accgcctcgc ccacgaaatc
     1681 aaggaactga ccgcgctggg cgtccaggtc ggcgtcgtga tcggcgcggg caacctggtg
     1741 cgcggcagcg ccgtccccga catgaaccgc gccgcggccg actatatcgg gatggtcgcg
     1801 acgatgatca acgcgctgat gatgcagggg gcgctggagc ggatcggcgt cgccacgcgc
     1861 gtgatgagcg cgatcgaaat gcgcgaggtg gccgagccct atatccgccg gcgcgcgatc
     1921 cgccatctcg atcgcggcga ggtcgtcatt ttcggctgcg gcaccggcaa cccgttcttc
     1981 accaccgaca cggccgccgc cctgcgcgcc aacgagatcg gcgccgacat cctgatgaag
     2041 gccaccaagg tcgacggcgt ctacgatgcc gacccggcga aagtgcccgg cgccgtcaaa
     2101 ttcgacgaac tgagctacga ccaggtcgtc cagcgcaacc tgcgcgtgat ggacacctcg
     2161 gccgtctcgc tctgccgcga caacggcctg ccgatcctcg ttttcaacct tgatcagccc
     2221 ggcaacgtga tgcgcgccgt ccgcggcgag aagatcggca cgctcgtgca ctgagcgggc
     2281 ctgttttttc tggccttgat ccgcccgcgt tttatcatgg agagggatac gttctggatc
     2341 agcccacccg aaccagggga gatggaacca tgcttgaggc attactgaag gaagcacgcg
     2401 aaaaaatgga caaggcggtg caggtcgtcg atcacgacct gagtgcgctg cgcaccggcc
     2461 gcgcgacgat ccacctgctc gacgggatca gcgtcgccat gtatggccag gaacaaccga
     2521 tcaaccagct agccaccgtg agcacccccg acgcccagac gatcgcgatc caaccgtggg
     2581 accgctcggc cctgggtccg atcgagaagg cgattctcca ggccaacatc ggcctgacgc
     2641 ccaacaacga cggcaagatc atccggctca acgttcctcc gttgaccgag gaaacccgcc
     2701 gcgacgtggt caagcgggcg catgcgatcg ccgaagaggg gcgcatcgcc atccgcaaca
     2761 tccgccgcca cgtcaacgac accctgaaga agagcgagaa ggaggccggc gtcagcgagg
     2821 acgagaagaa acgccttctg gatcaggtgc agaagctcac cgacgagcac atcaagcagg
     2881 tcgacgagca tatggcggcc aaggaagccg agatcatgaa ggtgtaaggg aaatcccgcg
     2941 aggtcacgcg aactgccgcc agatggattc atccagacga agcttttggg agtgcggcgg
     3001 cagagaccgc tcagtggtgg tcgccgagcg gcgccgcgct gcgcatgctc cagatcctgc
     3061 accggcccga aacgattgcg ccgctcgtta acttccggaa gttcaatgac aagcgcaaac
     3121 acgccggcaa tcggcgcggg attttcacgg caggttagct ttccgtccgg cgcagcgctt
     3181 tgagcttggg atcacgcctc ggtccgctcg atgagcagaa tcgccatgtc gtcgtgcggt
     3241 tcctcgccgt cggcgaactc ctcgagcgcc tgcaacacct ggccgacggc gcgatcgacc
     3301 ggcagcgcgg tggtggcggc gacggcctgt tccagccgct cggccccgaa ttcctccccc
     3361 gcgccgttgc gccgttccac cagtccgtcc gtgtataaaa acaaccgctc gcccggcttg
     3421 aacggatgcg cgacgacctg gaatttccag ttcgccacca gccccagcgc caggttcgac
     3481 ggcagttcaa gcggcgcgac gcgcatcggc tcggcggccg ccggccggtg cgtccacagc
     3541 ggcgcgggat ggccggcatt ggccagcaac agccggttct gatccagatg aatcacgccg
     3601 agcaggcagg tgatgaactg gcccggaatc agaatctcgg ccagcgaatg attgacctcg
     3661 gccatgaaag cctgcaggca atcgacgtgg tagcggtcgc tgacgagcgc cgccgagccc
     3721 cccgccaccg ggcggatcag cgcctcgctg cgccggtcga tcagcatctt gagcgccgag
     3781 gtcagcagcg ccgaggaaac cccgtgcccc gagatgtcgg cgatcatgaa gatcgccgtc
     3841 tggtcgtcga cgcggagcag gtcgtaaaga tcgccgccga tcagccggct cgcccggtag
     3901 aaactcgcgc accgcagccg gtcctggaac ggatagtcgc gcggcaggaa acgctgctgg
     3961 acttcctggg cctggcgcag ctcgcgtccc atccgccggt cgcggtcggt cagttccgcg
     4021 acggtcgcgg ccaggcgccg gttcgcctcc tggagctgat cgttctggct gcccacgcgc
     4081 tcgtgcaggc ggccgacgat ctccagcgcc tgcgccttgc cgtagagcgc gaagatcgtg
     4141 accgtaacca gaccgagcag aaagatcagg gcgaggaacg cgacgacgtt gccggcgaag
     4201 aactttgcgc ggacggtctg ttgcgtaatg tcgatgccga gcacggcgat gggccggccg
     4261 gtggattcct cgcggatcgg cgcgaacccc gacagatacg tcccccattc atcgcgaaaa
     4321 tacccgctgg tcgccgtcgg tcgcttcagc gccagggcga tcgccggctg gcccgccgcc
     4381 tcttcgtagg gctctccggg cagcaccccc cgctcgctcg gctgaatcgt gccgtcctgg
     4441 ttcaggtcga cgtcgtgggg ctgcgcatcg atcacgaacc gccacgaggt cggctggtcc
     4501 accgagtcgg gacggatgat gtaaatgtag cggacgtcgg ggtagacgtc cttcaccgtg
     4561 cggatctgca tcagaatccg tcggaaggcc ggcttgtccg tgtcgctcgg gtcgcggatt
     4621 tccgccagcg ccgccgggtc gatctgcgag ctgagcagcg tcgccagatc gcgcgccttc
     4681 gtccgcacct cttccttcag gatgccgtag cccgttcgca gcatgatcaa cacggtcagc
     4741 gtgacaagca ggtagaccgt ggcgagcgcg aggagccggc gcggcgtcag caggcgcgac
     4801 gcgcggaccg gatcgggcga ggggagcggg tcgtcgctgt tcagcatcgg tctcccatgg
     4861 taggcgagcc gggcggcggg cgcacctgca aaagaccccg cggcacgggt ccccgcgagg
     4921 ctcagtcggc cggaagcaag cccccttgag ctttgcctcc cttgtctcgt ttcacctcca
     4981 ggcgtgccgc gagggggctg attcggccgc tcccttatta agacgaacgc atcctttttg
     5041 gccgccggca tccccgtttc cccgcgcaat gcatttgcat cggacgcgtt ttttccagta
     5101 gacttctatc gtttggcaac cggtctttac ggttttgatt ctgcacgaga tgcgccatcc
     5161 cggtgccgcc gggttggtgc aggttgttcg tggtccacct ggaaaggtcg c
//
LOCUS       DAOPCO010000281         5195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13566_length_5195_cov_5.291440, whole genome shotgun sequence.
ACCESSION   DAOPCO010000281 DAOPCO010000000
VERSION     DAOPCO010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13566_length_5195_cov_5.291440"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLS90_14745"
     CDS             <1..226
                     /locus_tag="PLS90_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03704.1"
                     /translation="LRIQREGSILKLYYGQGYLAEADQFVGQRDYSAHQLPTVVGLLS
                     APGVASFKAEAELFLVGDFVPVELSGIWVE"
     gene            340..1005
                     /locus_tag="PLS90_14750"
     CDS             340..1005
                     /locus_tag="PLS90_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03705.1"
                     /translation="MKNRKQSSGVAFTLIELLIVVAIIAILAAIAVPNFLEAQARAKN
                     SRALADMKSLVTATSAYMVDYNDVPDPDGAFTSDAYNRLWWGFGSHRLTTPVTYMTSI
                     PVDIYPDETNKILDHWTRIPGNTLFNAPYQVLIRVDSSAPLGPKNIIVLGNPAAGDWQ
                     ALGLDPVTQQRIQASHAVYLSAGPDGVMTWQGFGGGGSPSAAVYDPTNGTISRGELYA
                     PVG"
     gene            1184..2518
                     /locus_tag="PLS90_14755"
     CDS             1184..2518
                     /locus_tag="PLS90_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03706.1"
                     /translation="MAALLDGFDGSTYNAGAQWLDLQVSQGTAADTVTLDQTTNAGKL
                     TITATNVAGDQTSLILHASETLDVGEYVQAKVTVLSGATRIGLCLAATTTITNRANLI
                     YYGLRNDGITRGHAYGDGGSEIWDAPATSIPGWDFGVEVTFKIVRSAEREYQCWWGLN
                     GNAENFDGSVIWPESGIPPIHVGFMFDPGLADFQGTVDDFETDLAGGGPTPTPTPTPT
                     PEPTPPPPNRTLDDFNDGSFDTAWIFSLPQINGGDGPALTYDESSVAGKLRVDFNADN
                     GGGFAFLLREDAFLEVGEAAYLDARQIEGTVGDLAIWFGMALATRTGEDQRVNVAYYT
                     IRNDGILRGLFFGDGGAEIGGVEPALGSYGIGDVITFVVHRVTETRFDFWYTLDDMPL
                     TRAGSGTWGGGSVPPIFAGLCLGVSENCVIEFDNFQMYKTPVNSVSGWTVIE"
     gene            3069..5054
                     /locus_tag="PLS90_14760"
     CDS             3069..5054
                     /locus_tag="PLS90_14760"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK03707.1"
                     /translation="MLELLRLGVRPLLWFAPGFLLWFGLPIASAQSVQVQWLLNFEQA
                     STGALGSTYVPDAGVGEVAPNGGEIRIVNNSGRPAIETLPNGRRGLRLDGRGAKDDGL
                     LFEHWLEGHPSRILEAVVAPDPDAPPERQTLIGSNDWDMRPPGRIVALGIDEFGLPTV
                     ELRSLDAYDHYPVTEELDSMHWYVKGTRPLAGGGAPVHLGMVYDHARQALYLYRDGQL
                     VRDARLDGDEGEFSIYRAVGISNWALIGEPVRGFTGHIDAVAESIFSGEFRPSMFQLI
                     EAEGPAPDPPAPSPRISLTLPDSWETSSRLPGERHPVFAPMSWDEPTYHHGPILTFWK
                     ERLYVAWHSAPRGENTWPPTGLVCSSTQLDDWSQPTKFPVVPRYLHATPDALYLWGGE
                     GRIFETRDGEHWEEIGPDRMAQYRRDNPVDIFAGTSHLPFQKKNPRQTYLSAGVREAG
                     AGDPFIALSDGRLMAAMLQETSHHEANSKAQWVCAPTTLDPSGLTGWSGGGIDLSACP
                     APGPAAGWQGPDGALHYVSRCGPRLWHAASADGGQTWSPLTIQPEFTDSPSRKVFGML
                     PNGQVYYLGNPIPNSRAQLVLALSDDGWHFDRARVVRWEPVAPQFPSPRKNYQPGYEH
                     VSAVVHDGKLYAVYSICRERIELSVVDLNDLNGGSKP"
BASE COUNT          985 a         1528 c         1656 g         1026 t
ORIGIN      
        1 gctgagaatc caacgcgaag gttcgattct caaattatac tacgggcagg ggtacctggc
       61 cgaagccgat cagttcgtgg gtcagcgcga ttacagcgcc catcaactgc cgaccgtggt
      121 cgggctgctc tccgcccccg gcgtggcaag tttcaaagcg gaagcggagc tgttcctggt
      181 cggcgatttc gtgccggtcg aactctccgg gatttgggtg gaatgataat cgggcgtcat
      241 tgatcggggt atctgccgct gcccgtgacc ggagcgaccg ggacagcggc ccgcggcagc
      301 ggcagaaatc cttcccaaac cgctgaagga ggacgtgcga tgaagaatcg gaagcaatcc
      361 tcgggcgtgg cgttcacgtt gatcgaactg ctgatcgtgg ttgcgatcat tgcgatcctg
      421 gcggccattg ccgtgccgaa ttttcttgag gctcaggccc gcgccaagaa cagccgggcg
      481 ttggccgaca tgaagagtct cgtcacggca acatccgcct acatggtgga ttacaatgat
      541 gtgccggacc cggacggcgc ctttacctcc gacgcgtata accggttgtg gtgggggttt
      601 ggttcgcatc ggctgacgac cccggtgacg tacatgacct ccattcccgt ggatatatat
      661 cccgacgaaa ccaacaagat cctggaccac tggacgcgta tcccgggcaa tacgctgttc
      721 aacgctccct atcaggtgtt aatccgggtg gattcgtcag cgccgctggg cccgaagaac
      781 ataatcgtat taggcaatcc ggcagcgggt gattggcagg ctctgggatt ggatcctgta
      841 acccagcaga ggattcaggc ctcccatgcc gtttatctga gcgccgggcc cgacggcgtc
      901 atgacctggc agggattcgg cggcggggga tctccgtcgg cggcggtcta tgacccgacc
      961 aatggaacga tttctcgagg tgaactttat gctccggtcg ggtaaagcga gcgggcaggg
     1021 caatgggttt cctgccaggt tcgagaatct aaattgcgat ggggggagag cagtgaacag
     1081 attgaattca tgaaaggaaa aggaaatgaa ggcaagatca acacatctta tcgtgaggcc
     1141 tttcataagt cttgcgtgtc tgtcgctcct gtcgatcgcg gcgatggcgg ccctcctcga
     1201 tggtttcgac ggatcgacct ataatgccgg ggcgcaatgg ctggacctgc aggtctcgca
     1261 gggaaccgcg gcggatacgg tcaccctgga tcagaccacg aatgcgggca aactgacgat
     1321 cacagcgacc aatgtcgcag gcgatcagac cagtctcatt ctgcatgcat cggaaacgct
     1381 cgacgtcggc gaatacgtgc aggccaaggt gacagtgctg agcggagcca cccgcatcgg
     1441 attatgcctg gccgccacga cgaccatcac caaccgcgcg aatctcattt attacgggtt
     1501 gcgcaacgat gggattacac gcgggcatgc ctacggggat gggggaagcg aaatctggga
     1561 tgcgccggcg acgtccatcc ccggctggga tttcggcgta gaggtgacct tcaaaatcgt
     1621 ccgctcggcc gagagggagt accagtgctg gtggggcctg aatgggaacg cggagaactt
     1681 cgatggctcg gtgatctggc ccgaatccgg cattcctccc attcacgtcg gtttcatgtt
     1741 cgatcccggt ttggcggatt ttcaggggac agtggacgat tttgaaaccg atctggcggg
     1801 cggcgggccg acgccgaccc caacgccgac tccgacgccc gagccgacgc cccctccgcc
     1861 caaccgcacc ctcgatgatt tcaacgatgg gtcattcgac acggcgtgga tcttcagcct
     1921 gccgcagatc aacggcggcg acggtccggc cctgacttac gacgagtcca gcgtggccgg
     1981 caagttgcgg gtcgacttca atgcagataa tggcggcggg ttcgcattcc tgctgcggga
     2041 agacgcgttc ctggaggtgg gcgaggcggc ctatctggat gcgcgtcaga tcgaggggac
     2101 ggtcggcgat cttgcaatct ggttcggcat ggcgctggcc acgcggaccg gcgaagacca
     2161 gcgcgtcaat gttgcttact ataccattcg caacgacggc atccttcgcg gtctgttctt
     2221 tggagacggc ggcgccgaaa tcggcggggt ggagcctgct ctgggaagct acggcatcgg
     2281 agacgtgatc accttcgtgg tccaccgcgt gaccgaaacc cgcttcgatt tctggtatac
     2341 gctggacgac atgccgctga cgcgagccgg atcaggaaca tggggcggcg gcagcgttcc
     2401 cccgattttt gccggtcttt gtctcggcgt ttccgaaaat tgcgtgattg agtttgataa
     2461 tttccaaatg tacaagactc cggtcaattc cgtttcgggt tggaccgtga tcgagtagct
     2521 gtgcgcaggc tggcgcatcc ggggcgcggg agtccaacga ctctcgcgcc cagtcccgcg
     2581 ccctgtcccg gtaatcccct ttggttcgag cgccaccccg gggaccgcag ccgccgccaa
     2641 gtcaccacca ttcccgtcat gaatccggtt cgggattgag atgctcccat ccgttcgcaa
     2701 gctgatgctc gtggccgcca gaacctggga actgcatgta ttccgagaat tgtgcagatt
     2761 gcccggatcc gacaggcggc ctcggcttcg gaggtgaccc agttgcgcca ttgaattccg
     2821 gcccggatgt tcttggcaag ccggcccttg tccaggaagc gcatactttt gatgacggcg
     2881 atctcgtgcg tgacaccgct ggtcggcttg aggtccggcg agcggtagac tcctggcgga
     2941 tcgtcacgcc ctcttcttcg cgcgcctcct gaaggccgtt gccgcagctg cagatatatc
     3001 ggctttaagg gtcattgcca cagtcgcagt agactctctc gtatccattt gaccgaggag
     3061 caaagccaat gctggaacta ctgcggctcg gggtgcggcc gctgctatgg ttcgcgccgg
     3121 gattcctcct gtggttcggc ttacccattg caagcgccca atcggttcag gtgcaatggc
     3181 tgctcaattt cgagcaggcg tcgacgggtg cactgggttc aacctacgtg cccgacgcgg
     3241 gtgtcggcga ggttgcaccc aatggaggcg agatccggat tgtcaacaac agcggccggc
     3301 cagccattga aactctaccc aacggtcgac gcggcttaag gctggacggc aggggtgcga
     3361 aggacgatgg gctgctcttc gagcactggc tcgagggaca cccttcgcgc atcctggagg
     3421 ctgtggtggc gcccgatccc gacgcgcctc ccgagcgcca gacgctcatc ggttcgaacg
     3481 actgggatat gcgaccgcca ggccggatcg tcgcattggg gatcgatgag ttcggactgc
     3541 ccacggtcga actgcgcagc cttgatgcct acgatcacta tcccgtgacc gaagaattgg
     3601 attcgatgca ttggtacgtc aagggaactc gccccctggc gggcggaggt gcgcccgtgc
     3661 atctcggcat ggtttacgac cacgcgcggc aggcgcttta tttgtatcga gacggccagc
     3721 tcgttcgcga cgcaaggcta gatggtgacg aaggcgagtt ttccatttat cgagccgtcg
     3781 gcatatccaa ctgggcgctg atcggcgagc ccgtacgggg tttcaccgga catattgatg
     3841 ccgttgccga atcgatattt tccggagagt ttaggccctc gatgtttcag ttgatcgagg
     3901 cagaagggcc cgcgcccgat cccccggcgc cctccccgcg catttcacta accttgcctg
     3961 attcatggga aacgtcaagc cgcctgccag gcgagcgcca tccggttttt gcaccgatga
     4021 gctgggatga gccgacgtat catcacggcc ccatcttaac tttctggaag gagcggcttt
     4081 acgttgcctg gcactcggcg ccgagaggcg agaacacctg gccgccgacc ggtctggtct
     4141 gttcatcgac gcagctggac gactggagcc aacccacgaa atttccggtt gttccacgat
     4201 atctgcatgc cacgcccgac gcgctgtatc tgtggggcgg agagggtcgc attttcgaaa
     4261 cccgggacgg tgagcactgg gaggagattg gcccggatcg aatggcgcaa taccgccggg
     4321 acaatccggt ggacatcttc gccggaacga gtcatctgcc tttccaaaaa aagaatccgc
     4381 gacaaactta cttgagcgcc ggcgtgcgcg aggccggcgc tggcgacccc ttcattgcct
     4441 tgagcgacgg tcgattgatg gccgccatgc ttcaggaaac aagccatcat gaggcgaact
     4501 ccaaagcgca gtgggtttgc gcaccgacca cgctggatcc gtcgggattg accggatgga
     4561 gcggcggggg gatcgattta agcgcgtgcc ccgcgcccgg gccggccgcc ggctggcagg
     4621 gtcccgatgg ggcgctccac tatgtgtcgc gctgcggtcc gcggctatgg cacgccgcga
     4681 gcgccgacgg cgggcaaacc tggagcccgc tgacgatcca gccggagttc accgacagcc
     4741 ccagccgcaa ggtgttcggc atgctgccga atgggcaggt gtactatctc ggcaatccga
     4801 tccccaacag ccgggcccaa ctggttctgg cgttgagcga cgacggctgg catttcgatc
     4861 gggcgcgagt ggtgcggtgg gaaccggtcg cgccgcaatt cccgtcgccc cgcaagaatt
     4921 atcaaccggg ttacgaacac gtcagcgccg tggtgcacga cggcaagctg tacgcggttt
     4981 acagcatctg ccgggagcga atcgaattga gcgtcgttga tctgaacgat ctgaacggag
     5041 ggagcaagcc atgatgaagt tttggcgcca gcgccggcgt gaatggcttc cggccgcgcg
     5101 ggggctatgg atccctgcgg catggctgct caccgggatg ccggtcacgg ccgcgccgac
     5161 cgagactccc gccgtgcaaa tccaatggct cttca
//
LOCUS       DAOPCO010000282         5190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13584_length_5190_cov_4.764752, whole genome shotgun sequence.
ACCESSION   DAOPCO010000282 DAOPCO010000000
VERSION     DAOPCO010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13584_length_5190_cov_4.764752"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2318
                     /locus_tag="PLS90_14765"
     CDS             <1..2318
                     /locus_tag="PLS90_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03708.1"
                     /translation="SMPPYRAGCTQRVRTAQGWRWITWESYGIHDDYGNLVEIQSVGR
                     NITQHMRTEQRFGTLVASLPGAVYRIECRKGWNIEYVSNAIADICGEPAGQFKRHRLH
                     TFVSLIHEEDRPRIWRQIRAAVRDRAAYQLEYRILHANGGIRWVTDKGQVVLSEENIL
                     YLDGVIFDNTEQKRAEIELKKYNENLELVRATLERQTAELEQKAQELDQAREAAEAAN
                     RAKSEFLANMSHEIRTPMNGVIGMTTLLLETELTREQREYAETVRKSAEALLVIINDI
                     LDFSKIEAGKLMLDENDFDLHEVLDETLALPRRQAQWKGLALELDLDKRIPRCIRGDS
                     GRLRQILTNLVGNAVKFTERGSVRLEIRQGAVRDQRLELTFRVIDSGIGIAPEIQPML
                     FQPFTQADGSMTRRFGGTGLGLAICKQLVTMMGGTIGVDSEPGFGSTFWFTLNLARAS
                     SPRDLSPDPATPDPAVLEAAAPTCHGLHVLLAEDNLINQKVAVRLLERLGCVLEVVCN
                     GRQAMEALEERQFDIVFMDCQMPELDGYEATMRIRALEAKRGGHIPIIAMTAHAMEGD
                     RARCLDVGMDDYISKPIQVQQLAELVAKWAERCGKAAPLDDLPVNATICKDDFVAPPV
                     RPAAPPPPAAVKSAPPVTEIKTGEPAPSDSPPIDRQVFEELCELMEDEGSSMMLDLLN
                     GFVADAREKVASIVDGVHSGDPRKIEQNAHTLKSSSGHVGATAMSALCYELQIAGREA
                     ALDLARPLAERLAAEFERVRHALLQEQQRFS"
     gene            complement(2406..4769)
                     /gene="gltA"
                     /locus_tag="PLS90_14770"
     CDS             complement(2406..4769)
                     /gene="gltA"
                     /locus_tag="PLS90_14770"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPK03709.1"
                     /translation="MDASSFRIRSRREIAPDTFLFEVEAPLVARARRAGQFMIITPTP
                     DSERIPISLAGGSREEGTIVFAVQRVGRTSNELCALQAGECFFSILGPLGEPTAIADE
                     YDGAVLCIGGGYGAGAVVPIAAAARAKGHRVIGIIGARDAQRLIFTSEMAAAAEELIE
                     VTEDGSVGRRGRVTDVVGDLLAKGIKIAHVFAIGPVPMMRALAEQTRPLGIGCTVSLN
                     ALMVDGTGMCGGCRVSVGGQTRFACCDGPDFDAHQVDFDLLVSRQRMYHAQERQAAAY
                     SDNGRPAADHRCHLDAMADAFFDIYRPVLPEGIDLDATEPLKPKDRMRIPRQRMPEQE
                     PAARIRNFAEVALGLSPLQAMAEARRCLECKHPYCIEGCPVNIEIPSFLKLVAQGDFA
                     AAARKVKESNCLPAICGRVCPQERQCEARCVLNKKAESVAIGRLERFVADFELSMEAA
                     GNSIQLAAPSGKRVAVVGSGPAGLTVAGELARRGHQVVVFEALHKPGGVLVYGIPEFR
                     LPTAVIDAEVENLRRMGVQFITGALIGRSLTLRELKEQEGFDAIFLGTGAGLPKLMGI
                     EGEDLKGSYTANEFLTRINLMRADLFPRQATPVHVGRHVVVIGGGNTAMDCARVARRM
                     GSEEVTILYRRTEAEMPARVEEIQHAKEEGIRFHFLAAPVRLMGDEDGWVSAIACEQM
                     ELGEPDASGRRRPVPVPGSEFVLPAETVVSALGFGVNPLLAATTPELKTNKWGIVEVN
                     DENGTSIPGVYAGGDVITGGATVILAMGHGKRAAQAIDEYLRQGAPA"
BASE COUNT          902 a         1933 c         1520 g          835 t
ORIGIN      
        1 tctcgatgcc cccctatcgc gccggctgca cccagcgcgt caggaccgcc caaggctggc
       61 gctggatcac ctgggagagc tacggcattc acgacgacta cggcaacctg gtcgaaattc
      121 agtcagtcgg ccgcaatatc acccagcaca tgcgcaccga gcagcgcttc ggcacgctcg
      181 tcgccagcct ccccggcgcc gtctaccgca tcgagtgccg caagggctgg aatattgaat
      241 acgtcagcaa cgccatcgcc gacatctgcg gcgagcccgc cggccagttc aagcgccacc
      301 gcctgcatac ctttgtctcg ctgatccacg aggaggaccg cccccgcatc tggcgtcaga
      361 tccgcgccgc cgtccgcgac cgcgccgcct atcagctcga ataccgcatt ctgcacgcca
      421 atgggggcat ccgctgggtc accgacaagg gccaggtcgt cctctccgag gagaacatcc
      481 tctacctcga cggcgtgatc ttcgacaaca ccgagcagaa gcgcgccgag atcgagctga
      541 agaaatacaa cgagaatctg gaactggtcc gcgccacgct ggaacgccag acggccgaac
      601 tcgaacagaa ggctcaggaa ctggatcagg cgcgcgaggc cgccgaggcc gccaaccgcg
      661 ccaagagcga gttcctggcc aacatgagcc acgagatccg caccccgatg aacggcgtca
      721 tcggcatgac cacgctcctg ctggagactg agctgacgcg cgaacagcgc gaatacgccg
      781 aaaccgtgcg caagtcggcc gaggccctgc tcgtcatcat caacgacatc ctcgacttct
      841 cgaagatcga agcgggcaag ctgatgctcg acgagaacga cttcgacctg cacgaggtcc
      901 tcgacgagac gctcgccctc ccccgacgcc aggcccagtg gaaggggctg gcgctcgagc
      961 tcgacctgga caagcggatc ccgcgctgca tccgcggcga ctccggccgc ctccgccaga
     1021 tcctcaccaa tctggtcggc aacgccgtca agttcaccga gcgcggttcg gtgcgtctcg
     1081 aaatccgcca gggcgccgtc cgcgaccagc gcctcgagct gaccttccgc gtcatcgaca
     1141 gcggcatcgg catcgctccg gaaatccagc cgatgctgtt ccagcccttc actcaggccg
     1201 acggctcgat gacccgccgg ttcggcggca ccggcctggg cctggccatc tgcaagcagc
     1261 tcgtcacgat gatgggcggc acgatcggcg tcgacagcga acccggcttc ggcagcacct
     1321 tctggttcac gctcaacctg gcccgcgcca gctctccgcg cgacctctcc cccgatccgg
     1381 ctacgcccga tcccgctgtg ctcgaggccg ctgcgccgac ttgccacggc ctccacgtcc
     1441 tgctggccga ggacaacctg atcaaccaga aggtcgcggt gcgcctgctg gagcggctcg
     1501 gctgcgtcct ggaggtcgtc tgcaacggcc ggcaggcgat ggaagctctc gaggaacgcc
     1561 agttcgacat cgtcttcatg gattgccaga tgcccgaact cgacggctac gaggcgacga
     1621 tgcgcatccg cgcgctcgaa gccaagcgcg gcggacatat cccaatcatc gccatgaccg
     1681 cccacgcgat ggaaggcgac cgcgcgcgct gcctcgacgt cggcatggac gattacatca
     1741 gcaagccgat tcaggttcag cagttggccg aactggtcgc caaatgggcg gaacgctgcg
     1801 gcaaggccgc gccgctcgac gatctgccgg tcaacgccac gatctgtaag gacgacttcg
     1861 tcgcgccgcc ggtccgcccc gccgcgccgc cgccccccgc cgccgtgaaa tcggccccgc
     1921 cggtcacgga gatcaaaacc ggcgaaccgg cgccgtccga ttcacctccc atcgaccgcc
     1981 aggtcttcga agagctctgc gaactgatgg aggacgaggg ctcgtcgatg atgctcgacc
     2041 tgctcaacgg gttcgtcgcc gacgcgcgtg aaaaagtcgc ctcgatcgtc gacggcgtcc
     2101 actccggcga tccccggaaa atcgaacaga acgcccacac gctcaaatcg agcagcggcc
     2161 acgttggcgc caccgccatg tcggccctgt gctacgaact tcagatcgcc ggccgcgaag
     2221 cggccctgga cctggcgcgc ccgctggccg aacggctcgc tgccgaattc gaacgcgtcc
     2281 gccacgccct gctccaggaa cagcaacgct tctcctgaac ctcgattcgc ctccggcccg
     2341 cccattcccg cctcgggctt gctctcccta ctgacctctt tcgtgtcttt cgtgatcgga
     2401 actgtttacg ccggcgcccc ctggcgcagg tattcatcga tcgcctgcgc cgcgcgcttg
     2461 ccgtgcccca ttgccaggat caccgtcgcg cccccggtga tcacgtcgcc cccggcatac
     2521 acccccggga tgctcgtccc gttctcatcg ttcacctcga cgatccccca cttgttcgtc
     2581 ttgagctcgg gcgtcgtcgc cgccagcagc gggttgacgc cgaaccccag cgcgctgacg
     2641 accgtctcgg ccggcaggac gaactcgctg ccggggactg gcaccggtcg ccggcgcccg
     2701 ctggcgtcgg gctcgcccag ttccatctgt tcgcacgcga tggcgctgac ccagccatct
     2761 tcatccccca tcagccgcac cggcgcggcg aggaaatgga accggattcc ctcctctttg
     2821 gcatgctgga tctcctcgac ccgggcgggc atttcggcct ccgtccggcg gtagaggatc
     2881 gtcacctcct cgctccccat ccggcgggcg acgcgcgcgc aatccatcgc cgtgttgccc
     2941 ccgccgatga cgacgacatg ccggccgacg tgcaccggcg tcgcctgccg cgggaacaga
     3001 tcggcccgca tcaggttgat ccgcgtcagg aattcattgg ccgtatagct ccccttcaag
     3061 tcctcgccct cgatccccat cagcttgggc agccccgcac ccgtgccgag aaagatcgcg
     3121 tcgaacccct cctgctcctt caattcccgc agcgtcaggc tgcgcccgat cagcgcgccg
     3181 gtgatgaact gcacccccat ccgccggaga ttctcgacct ccgcatcgat caccgccgtc
     3241 ggcagccgga attccggaat accgtaaacc agcacgcccc ccggtttgtg cagcgcttcg
     3301 aagaccacca cctggtggcc gcggcgcgcc agttcaccgg caacggtcag cccggccgga
     3361 cccgacccga caacggcgac ccgtttgccc gacggcgccg cgagctggat cgagttgccc
     3421 gctgcctcca tgctcaactc gaaatcggcc acgaaacgct ccagccggcc gatcgcgacc
     3481 gattcggcct tcttgttcaa cacgcagcgc gcctcgcact ggcgctcctg cgggcatacc
     3541 cgcccgcaga tcgccggcag gcagttggac tccttgacct tgcgcgccgc cgcggcgaaa
     3601 tccccctgtg ccaccagctt cagaaacgac gggatctcga tgttgaccgg gcacccttca
     3661 atgcaatagg gatgcttgca ttccaggcag cggcgcgctt cggccatcgc ctgcagcggc
     3721 gacagcccca gcgccacttc cgcaaaattg cggatccgcg ccgccggctc ctgctcgggc
     3781 atccgctggc gcgggatgcg catccgatct ttcggcttga gcggttcggt ggcgtccaga
     3841 tcaatcccct cgggcagcac cgggcggtag atgtcgaaga acgcatcggc catcgcatcc
     3901 agatggcagc gatgatcggc cgccggccgc ccgttgtcgc tgtacgccgc cgcctgccgt
     3961 tcctgtgcgt gatacatccg ctggcgcgag acgagcaggt cgaaatcaac ctgatgcgcg
     4021 tcgaaatccg gaccgtcgca gcacgcaaat cgcgtctgcc cgccgacgct gacgcgacag
     4081 ccgccgcaca tccccgtgcc gtcgaccatc agcgcattga gcgagaccgt gcagccgatg
     4141 cccagcggcc gggtctgctc ggccagcgcg cgcatcatcg gcacgggacc gatcgcaaac
     4201 acgtgggcaa tcttgatccc cttggccagc aggtcgccga cgacgtcggt gacgcggccg
     4261 cgccgcccga cactcccgtc ttcggtgacc tcgatcagtt cctccgcggc ggcggccatc
     4321 tcgctggtga agatgagacg ctgcgcatcg cgcgcgccga tgatgccgat gacgcgatgg
     4381 cccttcgcgc gcgcggctgc cgcgatcggc acgaccgcgc ccgccccgta gccgccgccg
     4441 atgcacagga cggcgccgtc atattcatcg gcgatcgccg tcggctcgcc cagcggcccc
     4501 aggatgctga agaaacactc cccggcctgg agcgcgcaca actcgttgct cgtgcggccg
     4561 acgcgctgca cggcgaagac gatcgtaccc tcctcgcgcg agccgcccgc caggctgatc
     4621 gggatccgct cgctgtcggg cgtcggcgtg atgatcatga actggccggc gcgacgcgcg
     4681 cgcgccacca gcggcgcctc gacctcgaac aggaacgtat cgggagcgat ttcgcgccgg
     4741 gagcgaatgc ggaaggagga agcgtccatg gaacctgcct cggggagttg aggactcaaa
     4801 catccgtaac ggaatctatc aggcgccagt cacgaattca agactctttt gtctacaatc
     4861 agcacgatct gcctgccgcc tctccaagca tccatcctag ccgttgtcct cgtgatcgcc
     4921 gcctccgccc cccgtttctc gccttgcatc ggccctctgt ttaggtgttt attgtaatca
     4981 tctgcaatgt caccggagtc agtctgggac ccatggcgct tgaacacgaa ctgggctata
     5041 ccgagccgat ccgcaatcac ctccagcgct ccctgctggc catcgtgctg accggcagtc
     5101 aactggccaa ggaggccgac cgcctgctgc gcccgttcgg cctgaccgaa gcccagttca
     5161 acgtcctgat gctccttaaa tatcaggccg
//
LOCUS       DAOPCO010000283         5185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13599_length_5185_cov_5.040546, whole genome shotgun sequence.
ACCESSION   DAOPCO010000283 DAOPCO010000000
VERSION     DAOPCO010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_5185_cov_5.040546"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..334)
                     /locus_tag="PLS90_14775"
     CDS             complement(41..334)
                     /locus_tag="PLS90_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HPK03710.1"
                     /translation="MNQIKSILSITAIALTLTFGTIGCETIRDNPGAATGAAIGAGTG
                     AVLGGVVGSQSGDTATGAVIGGVTGAGVGGVIGHQHDKQEEQDRRIDRLERRY"
     gene            844..4191
                     /locus_tag="PLS90_14780"
     CDS             844..4191
                     /locus_tag="PLS90_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK03711.1"
                     /translation="MLRTCIENPVKVTMFFAGLVLLGWLSYRRLPLNLFPDVRTPRIT
                     VVVTTDGLPPQQAERLLTMDIERQMTSIKGIASVTSYSRDRNIVLHVDFHWGQDMEFA
                     YLDVKKNISQFETNEHVEQIDVYRFDPNAAPLLTLAFVPPAGGRIERVALTSFVENSL
                     KPRLETIEGVAYVKTAGDREEEVFVEVNEELMSQYQLTSAQILQAIEQSNLATPAGVV
                     VENNREMLLKFINRLSGLDEIGQVIVKTVNDYPVRLIDIARLSTRPVRDEVLVRQDLA
                     ESIALRIYREPEANAVLTARRVREAVDELNRRGGYGLKIATDLSRDIESAIAEVVDTA
                     LVGMGLAMLVLLVFLRNLRATLAVGFAIPLSILGTFSLMYFQHLSLNIMTLGGLALGA
                     GMLVDNGIVVIENVFRHRQAGAEAKTAALEGTREVAMAITASTLTTIVVFVPLVYVHG
                     IAGLLFKDQALTVVYSLLVSLVVALGLLPMLAARLGRQQPRPPGRLDRVYGGFLAGSL
                     RWRWLLLLAFAGGLYWTWLAGRTIPREFFPKAVVGRFSAAITLPPGSTLEQTAADVAR
                     LERALEAFRYRDPRLAPLLRAWERYERDGLAGPLIAAADGWHERVAPPPDLPAELRAQ
                     LAADQPPTIDVFLAHRDWLDEQIILRSVTTIIGSDPTGVEAAREKILGPHTATIDVTM
                     NPGVLRELAAEDLIERLRAEAALHPEMEISFEAKSEFLQELLGRERGDIVLEVHADRL
                     EHLMTSAERVAQALRTIPGLVNVRTNLTLGEEEFVLTPDRDALLRDGFTVEGLATQIQ
                     AYLQGNQSERLRFDEGDMIVTVSPGRAEEAGLRGLLDLPILPGDGQAGSLGGANAGER
                     QKLRNLVSVGTTRSLRELMRVNQEKTLLVMADREGVRYQDAIAAIGQALAAVPWPLGA
                     TWNLSGEEVQRRESFDRLFFALMIAIVLVYMVIAAILESIIHPLTIMLSVPFALAGVV
                     AAFILTGVSLNLMGYIGIVMLVGIVVNNAIVLLDRVNQLRMEGATARQALIDAGRQRL
                     RPILMTSLTTILALVPLALGYGEGAELRRPMAIAVIGGLGASTLLTLWLMPGIYLCVE
                     DIRSVFRRGRGLFARRAVEPEPEARGS"
     gene            4216..>5185
                     /locus_tag="PLS90_14785"
     CDS             4216..>5185
                     /locus_tag="PLS90_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03712.1"
                     /translation="MQPIPVARSHCTSMIRSLLTIGRYEFRMHLASWRWRGLALLALA
                     FNFGVYQAALFQHSFLPSDVLVSRWVLPLYLLAVVFIGLYSMARIRETGIRPLIMTKA
                     FPTMVLPAGQWLGGMLALALLFGLGLFPAAVALRFTTDIVVYLRPMGWMLVFNLLPGA
                     GLVLACTLWIRACFRHNLTALIILGALGGGLTFVANRYFVVGGEPFVFQFHPLLAPFA
                     DPWWRRLRDVELQSMIPLLDPAEWRRVAWMLGAVTLFLHLTTYHLRRTEPQRKVLGTY
                     GASWADMPTFVRLLADLRVDPHVGWRAHLVLGVMLAARLIPARRRLA"
BASE COUNT          783 a         1625 c         1829 g          948 t
ORIGIN      
        1 tcaggaatca agccgcgact tgccttggcc ggctggggga tcagtaacgg cgctcgagcc
       61 gatcaatccg gcggtcttgt tcttcctgct tgtcgtgctg atggccgatg acgccgccaa
      121 cgccggcgcc ggtgacgccg ccgatcacgg cccccgtcgc ggtatcgccc gattgcgagc
      181 cgaccacgcc gcccaggacc gcgccggtgc cggcgccgat agccgccccg gtcgcggcgc
      241 ccggattgtc gcggatggtc tcgcagccga tcgttccgaa cgtcagggtg agcgcgatcg
      301 cggtaatgct caagatgctc ttgatctggt tcatgatact gtcctccgac aatctccggc
      361 ccggcgttgc cgcgccgcaa tcaacgggtc ctgatttcct agacctgccg gccccgccgt
      421 tgttcctcgc tttcgccccc tgtagagaga tcaagcaata gccttgccat gagggaaggc
      481 ggaatgacgt gggggaaaaa gagacataag ggatgtaaag gacgtaaaga acatatggaa
      541 caacaaataa ccgactgggg gaacttcggt acaaagccat tgagatcagc ggtgagctgc
      601 tggcagcatg gggcggaagc gaacggcaca atcgccgccg aggcgtcgtg gaaccatttg
      661 gccacaattg cgacttaaac cgtgggagga gtcgatttcg agggctcccg gggtcgccgg
      721 gattcgccct ctgcgagccg cgtttaagcg tccccgggtt cggccgagct tcatttggac
      781 ttgagcgatt atcgagagcc gaggacgagg gaccagagac gtcgttttga gtgaggggcg
      841 gggatgctgc ggacgtgcat cgagaatccg gtcaaggtga cgatgttctt cgccggcctg
      901 gtcctgctgg gctggctgtc gtatcgccgg ctgccgctca atctgttccc cgacgtgcgc
      961 acgccgcgca tcaccgtggt ggtgacgacc gacggcctgc cgccgcagca ggccgagcgc
     1021 ctgctgacga tggacatcga gcgccagatg acctcgatca aggggatcgc gtcggtcacc
     1081 agctactcgc gcgatcgcaa tatcgtgctc catgtcgatt tccactgggg tcaggacatg
     1141 gagtttgcct atctggatgt caaaaagaac atcagtcagt ttgagacgaa cgaacatgtc
     1201 gagcagatcg atgtctaccg gttcgatccg aatgcggcgc ccctgctgac gctggcgttc
     1261 gtgccgccgg ccggcggccg gatcgagcgg gtcgcgctga ccagtttcgt cgagaactcg
     1321 ctcaagccgc ggctcgagac gatcgagggc gtggcctacg tcaagacggc cggggaccgg
     1381 gaggaagaag tctttgtcga ggtcaacgag gagctgatgt cgcagtatca gctcacctcg
     1441 gcgcagattc tgcaggcgat cgagcagagc aacctggcga ccccggcggg ggtggtggtc
     1501 gagaacaacc gcgagatgct gctcaagttc atcaaccggt tgtcggggct ggacgagatc
     1561 ggccaggtga tcgtcaagac ggtcaacgac tatccagtgc ggctgatcga tatcgcgcgg
     1621 ctgtcgacgc ggccggtgcg cgacgaagtg ctggtgcggc aggatctggc cgaatcaatc
     1681 gcgctgcgga tctaccgcga acccgaggcc aacgccgtgc tgacggcccg gcgcgtgcgc
     1741 gaggcggtcg atgagctgaa tcggcgcggg ggttacgggc tcaagatcgc caccgacctg
     1801 tcgcgcgata tcgagtcggc gatcgccgag gtggtcgaca cggcgctggt cggcatgggg
     1861 ctggccatgc tggtgctgct tgtttttctg cgcaacctgc gcgccacgct ggcggtcggt
     1921 ttcgccattc cgctgtcgat cctcggcaca ttcagcctga tgtatttcca gcatctgtcg
     1981 ctcaacatca tgacgctggg cgggctcgcc ctgggcgccg ggatgctcgt cgacaacggc
     2041 atcgtcgtga tcgagaacgt cttccgccac cggcaggcgg gggcggaggc gaaaaccgcc
     2101 gcgctggagg ggacgcgcga ggttgcgatg gcgatcaccg cctcgacgct caccacgatc
     2161 gtcgtcttcg tcccgctggt ctacgtccac ggcattgccg gactgttgtt caaggatcag
     2221 gcgctgaccg tcgtttactc gctgctggtg tcgctggtgg tggcgctggg actgctgccg
     2281 atgctggccg cgcgcctcgg acggcagcag ccgcgcccgc cgggccggct ggaccgggta
     2341 tatggggggt tcctggcggg gtcgctgcgc tggcgctggc tgttgctgct ggcgttcgcc
     2401 ggcgggctat actggacctg gctcgccggc cggacgattc cgcgcgaatt ctttcccaag
     2461 gcggtggtgg gccggttctc ggcggcgatt acgctgccgc cgggatcgac tctggagcag
     2521 acggccgcgg acgtggcgcg gctggagcgc gcgctggaag cgttccgtta ccgcgacccg
     2581 cgcctcgcgc cgctgttgcg ggcctgggag cgctatgagc gggacggcct cgccgggcca
     2641 ctgattgcgg cggcggatgg ctggcacgag cgggtcgcgc cgccgcccga cctgccggcg
     2701 gagctgcggg cgcaactggc ggccgatcag ccgccgacga tcgatgtctt tctcgcccac
     2761 cgcgactggc tggacgagca gatcatcctg cgctcggtga cgacgatcat cggctcggac
     2821 ccgaccggcg tcgaggcggc gcgggaaaag atcctcggcc cgcatacggc cacgatcgac
     2881 gtcacgatga accccggcgt gctgcgcgaa cttgccgccg aggacctgat cgagcggctg
     2941 cgcgccgagg cggcgttgca tcccgagatg gagatctcgt tcgaagcgaa aagcgaattc
     3001 ctccaggagc tgctggggcg cgaacggggc gatattgtgc tggaagtcca tgccgaccgg
     3061 ctcgagcacc tgatgacgtc ggccgagcgg gtggcccagg cgctgcgcac gattccgggc
     3121 ctggtcaatg tgcggaccaa cctgacgctg ggcgaggagg aattcgtgct gacgcccgac
     3181 cgcgacgccc tgctgcgcga cggtttcacc gtcgagggcc tggcgacgca gatccaggcc
     3241 tacctgcagg gcaaccagag cgagcggctg cggttcgacg agggcgatat gatcgtgacg
     3301 gtgagcccgg gacgggcgga ggaagccggc ctgcgcggcc tgctcgatct gccgatcctg
     3361 cccggcgacg gccaggcggg gtcccttggc ggcgcgaacg ccggcgagcg ccagaagctg
     3421 cgcaacctgg tctcggtggg gacgacgcgc agcctgcgcg agctgatgcg cgtcaatcag
     3481 gaaaaaacgc tgctggtgat ggccgaccgg gagggcgtgc gctaccagga cgcgatcgcc
     3541 gccatcgggc aggcgctggc cgccgtgccg tggccgctgg gcgccacttg gaacctgagc
     3601 ggcgaggagg tgcagcggcg cgagtcgttt gaccgcctgt ttttcgcgct gatgatcgcg
     3661 atcgtcctgg tctacatggt gatcgcggcg atcctcgagt cgatcatcca tccgctgacg
     3721 atcatgctga gcgtgccgtt tgcgctggcg ggcgtcgtcg cggcgttcat cctgacaggc
     3781 gtgagcctca acctgatggg ttacatcggc atcgtgatgc tggtcggcat cgtcgtgaac
     3841 aatgcgatcg tcctgctcga ccgggtgaac cagttgcgga tggagggggc gacggcgcgc
     3901 caggcgctga tcgacgcggg acgccagcgg ctgcggccga tcctgatgac ctcgctgaca
     3961 accattctgg cgctcgtgcc gctggcgctg ggctacggcg agggagcgga actgcgccgg
     4021 ccgatggcga ttgccgtgat cggcgggctc ggcgcctcca cgctgctgac gttgtggctg
     4081 atgccgggga tctacctgtg cgtggaggat atccgctcgg tgttccgccg cggccgcggg
     4141 ttattcgcgc ggcgcgcggt cgagccggag ccggaagccc gtggaagctg aggactcgcg
     4201 cgcgatgcgt tgccgatgca accaattccc gtcgctcgtt cccattgcac ctcgatgatc
     4261 cgctcactgc tcacgatcgg ccggtatgag ttccggatgc acctggcgtc gtggcgctgg
     4321 cggggactcg cgctgctcgc gctggcgttt aatttcggcg tctatcaagc ggcgcttttc
     4381 cagcacagct ttctccccag cgacgtgctg gtcagccgtt gggtcctgcc gctgtatctg
     4441 ctggccgtcg tcttcatcgg cctgtattcg atggcgcgca tccgcgagac cggcatccgc
     4501 ccgctgatca tgaccaaggc gttccccacg atggtcctgc cggcgggcca gtggctgggg
     4561 gggatgctgg cgctcgccct gctgttcggc ctgggcctgt tcccggcggc cgtcgcgctc
     4621 cggttcacca ccgacatcgt cgtgtatctg cggccgatgg gctggatgct ggtgttcaat
     4681 ctgttgcccg gcgcgggcct ggtgctggcc tgcacgctct ggattcgcgc ctgctttcgc
     4741 cataatctga cggcgctgat catcctgggg gcgctgggcg gcggattgac ctttgttgcg
     4801 aacaggtatt tcgtcgtcgg cggcgagccg ttcgttttcc agttccatcc gttgctggcg
     4861 ccgttcgccg acccctggtg gcggcgactg cgcgatgtcg agctgcagtc gatgatcccg
     4921 ctgctcgacc cggccgaatg gcggcgcgta gcgtggatgc tgggcgccgt cacgctcttt
     4981 ctgcacctga cgacctatca cctgcgccgc accgagccgc aacgcaaggt gctcggcaca
     5041 tacggcgcga gctgggccga catgccgacg ttcgtccggc tgctggccga tctgcgggtc
     5101 gacccgcatg tgggctggcg cgcccacctc gtgctggggg tgatgctggc ggcgcggctg
     5161 atccccgccc gccggcggct ggccc
//
LOCUS       DAOPCO010000284         5182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13605_length_5182_cov_5.457773, whole genome shotgun sequence.
ACCESSION   DAOPCO010000284 DAOPCO010000000
VERSION     DAOPCO010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13605_length_5182_cov_5.457773"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLS90_14790"
     CDS             <1..918
                     /locus_tag="PLS90_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03713.1"
                     /translation="LADEGYEVLTASDGPSGLEAYQQHRPAFTLTDISMPGMSGLDLL
                     KQIKELNPEAAVMLMTGAGSEAYAVEALRLGAVNYFNKPVDINELGNTLNRYAALAAG
                     FDYERYAAQFMVAEHLALCLDNDIDFVNHAVQMIINHCRSIFPLTEIFTLRFGLYEML
                     VNAIEHGNLGITYQEKSAALEQNRLGELLKERAAEPERAARRVHITCDIKPEGLTCRI
                     RDEGDGFDHSMYSNTTDPAQLFEELGTSLHGRGILLTSLQFDTVRFNDKGNEVFIFKR
                     SPNFPAPPPGTVARQAAPAELPSELELDP"
     gene            915..1865
                     /locus_tag="PLS90_14795"
     CDS             915..1865
                     /locus_tag="PLS90_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPK03714.1"
                     /translation="MKSWNLRCIALLAWILAGHLSLFAQPAADKPPLNTLGLETDLRD
                     PSAQPLSLPTLPLPGAETAYESETRYAPTARPLPEVPRPVERPIVGEAVEATTAGLTL
                     RGYLTRPSSPPFNGGLLLLPEFWGVNEATRQRADDFARYGYRVLVLDFYDGQPVASRG
                     DAARLMRELDRELTQRRIGAAAQWLSSTSGVRDLPVAIIGYGLGATLGLHAMTQPDPT
                     PPAVRGLVLFYGETIAEPGRLETLGIPIVGFYATEDFWVRPEKVAAFDDALTSAGVPH
                     QFFHFDTQTGFGLEPADYRQQGYHHAANDRILDFLARYLH"
     gene            1983..2450
                     /locus_tag="PLS90_14800"
     CDS             1983..2450
                     /locus_tag="PLS90_14800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03715.1"
                     /translation="MPLFSLLRRLRPARDPMAPISNVPLSEQQLADANAALGNGYLQA
                     GDYLRAIEAFTMAVLHDPQFARAYFMMGMAYCCLGQHRNAIGTYTQAILLRSDKAMYF
                     FGRARAYEELGEWDEAIADYESVLALEPDHGAALHQLEELRKRLDLPTESTVA"
     gene            complement(2514..3449)
                     /locus_tag="PLS90_14805"
     CDS             complement(2514..3449)
                     /locus_tag="PLS90_14805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPK03716.1"
                     /translation="MKEARQADLFGEMSESPEAQRPVRRKKGSPPVKPSKATVELIHG
                     DFLKRAPAVIADGSIDVIVTSPPYNLNIKYSKYNDDLPRQEYLAWTDRWAAEAKRCLA
                     PQGSLFLNVGVRPREPWGAWEVAEIFRRHFALQNVIHWIKSISIAKADVGDYPGIVDD
                     VTVGHYKPINSNRYVNDQHEYIFHLSHQGNVELDRTAIGVPYQDASNVTRWKSAAGGL
                     HCRGNTWFMPYETINFRDRDRPHPASFPLALPDRCLRLHGVRRIRRVLDPFAGLGTTL
                     VAAARLDLDAVGFDIDEAYLAVARDQLARTGCKVR"
     gene            complement(3604..4716)
                     /gene="ugpC"
                     /locus_tag="PLS90_14810"
     CDS             complement(3604..4716)
                     /gene="ugpC"
                     /locus_tag="PLS90_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPK03717.1"
                     /translation="MAQVVLKNVKKVYPGVPKPAVEQVSLEIQDKEFVVLVGPSGCGK
                     STTLRMIAGLEEITAGEIYIGDRLVNDVPPKDRDIAMVFQNYALYPHMTVYKNMAFGL
                     MLRKFPKAEIDRRVREAAEILGITEYLDRKPKALSGGQRQRVAVGRAIVRKPQVFLFD
                     EPLSNLDAKMRVQMRTEISKLHHRLQATMIYVTHDQVEAMTMGDRIVVMNDARIQQIA
                     EPIDLYDNPANKFVAGFIGSPPMNFIDGELVERGGTLHFVEESGTIDFPVYDAHVEAL
                     RNHNNKRVTFGVRPEDFFDPSYQKADPHYRELKARIEVVEPMGSEVYLYLTTGRHPFI
                     ARVEAHVRAETNDEKALLMNAAKGHYFDIETEKTIV"
BASE COUNT          904 a         1809 c         1534 g          935 t
ORIGIN      
        1 ctggccgacg agggctacga ggtgctgacg gccagcgacg ggccgagcgg gctggaggcc
       61 taccagcagc accgccccgc gttcacccta acggatatct cgatgccggg catgtcgggc
      121 ctggatctgc tcaagcagat caaggagctg aatcccgagg ccgccgtgat gctgatgacc
      181 ggcgccgggt ccgaagccta cgccgtcgag gccctgcgcc tgggcgccgt caactacttc
      241 aacaaaccgg tcgacatcaa tgaactgggc aacacgctca accgctacgc cgccctggcc
      301 gccggattcg actatgaacg ctatgcggcg cagttcatgg tcgccgagca cctcgccctg
      361 tgcctcgata acgacatcga cttcgtcaac cacgcggtac agatgatcat caatcactgc
      421 cgctcgatct tcccgctgac cgagatcttc acgctgcgct ttggcctcta cgagatgctg
      481 gtcaatgcga tcgagcacgg caacctgggc atcacctacc aggaaaagtc cgcggcgctg
      541 gaacaaaatc ggctcggcga actcttgaag gaaagggcgg cggaacccga acgggcggcc
      601 cggcgcgtcc acatcacttg cgacatcaag cccgaggggc tgacctgccg gattcgcgac
      661 gagggcgacg gtttcgatca ttcgatgtac agcaacacga cggatccggc ccagctcttc
      721 gaggaactgg gcacatcgct ccatgggcgc ggcatcctgc tgaccagtct ccagttcgac
      781 acggtgcgct tcaacgacaa ggggaacgaa gtcttcattt tcaagcgttc accgaatttc
      841 cccgccccgc cgccgggcac ggtcgccagg caggcggccc cggccgaact tccgtcggag
      901 cttgaattgg atccatgaag tcctggaacc tgcgttgcat tgccctgctg gcctggatcc
      961 tggccgggca tctttccctg tttgctcaac ccgccgcgga caagcccccc ctcaacacgc
     1021 tgggactgga aaccgacctg agggacccca gtgcgcagcc gctgagcctg ccgaccctcc
     1081 cgctgcccgg cgccgagacc gcgtacgaga gcgagacccg ttacgccccc accgcgcgcc
     1141 cgttgcccga agtgcccagg ccggtcgagc gcccgatcgt cggcgaggcc gttgaggcca
     1201 cgaccgccgg gctgaccctc cgcggctacc tgacgcgccc gtcgtcgccc ccgttcaacg
     1261 gcggcctgct cctgctgccc gaattctggg gcgtgaacga ggccacccgc cagcgcgccg
     1321 acgatttcgc ccgttacggc taccgcgtcc tggtgctcga tttttacgac gggcagccgg
     1381 tcgcgagccg cggcgatgcc gcgcggctca tgcgggaact cgaccgcgag ctgacccagc
     1441 ggcggattgg cgccgccgcg cagtggctca gttccaccag cggcgtcagg gatctgccgg
     1501 tcgccatcat cggttacggg cttggcgcca cgctcgggct gcacgcgatg acgcaaccgg
     1561 accccacgcc gccggcggtg cgcggcctcg tgctgttcta tggcgaaacc atcgccgaac
     1621 cgggccggct cgaaacactt ggcatcccca tagtcggctt ttacgcgacc gaggatttct
     1681 gggtgcggcc cgagaaggtc gccgcgttcg acgatgccct caccagcgcc ggcgtcccgc
     1741 atcagttctt ccatttcgac acgcagaccg gcttcggtct cgaaccggcc gattatcgcc
     1801 agcagggcta ccaccacgcc gccaacgacc ggatcctgga cttcctcgcc cgctatctgc
     1861 actgaccccg cgtcctctgc ctttgctgtt cgaaaaaaag aaacgcgatc atggatgcgg
     1921 cggccctgcc gatacgaagg ccaagcgaaa tgttcaacac gacaggatgt tgtgagccag
     1981 cgatgccttt gttcagcctc ctccgccgcc tgcgccccgc gcgcgacccg atggcgccga
     2041 tctccaatgt tccgctgagc gaacagcaac tggccgacgc caatgccgcg ctgggcaacg
     2101 gctacctcca ggccggcgat tatctgcgcg ccatcgaggc gttcaccatg gccgtcctgc
     2161 acgaccccca gttcgcccgc gcctacttca tgatgggcat ggcctactgc tgcctcggcc
     2221 aacatcgcaa cgccatcggg acctacaccc aggccatcct gctccgttcg gacaaggcga
     2281 tgtatttctt cggccgcgcc cgggcctatg aggaactcgg cgagtgggat gaggccatcg
     2341 ccgattatga aagcgtcctg gcgctcgagc ccgaccacgg cgccgcgctg catcagctcg
     2401 aggagctgcg caagcggctc gacctgccca cggaatccac ggtggcctga cccgctggct
     2461 cccctgcctc ggccgtcagg ggctcccgcg caatccacgc gccgcccccg ctgctacctc
     2521 accttgcacc ccgtccgcgc caattgatcg cgggcgacgg caaggtaggc ctcgtcgata
     2581 tcgaatccga cggcgtccag gtcaagtcgc gcggcggcga ccagcgtcgt tcccaacccg
     2641 gcgaacgggt ccaggacgcg ccggatgcgt ctcaccccgt gcaaccgcag gcagcggtcg
     2701 ggcagcgcca atggaaagct ggccgggtgc ggccggtcgc ggtctcggaa attgatcgtc
     2761 tcgtagggca tgaaccaggt attgccgcgg cagtgcagcc cccccgccgc cgatttccag
     2821 cgcgtcacgt tgctcgcatc ctgatacggc acgccgatcg ccgtccggtc cagttccaca
     2881 ttgccctggt gcgacaggtg aaaaatgtac tcgtgctggt cgttgacgta gcggttgctg
     2941 ttgatcggct tgtaatggcc gaccgtcacg tcgtccacga tgccggggta atcgccgacg
     3001 tcggccttcg cgatcgagat cgacttgatc cagtggatga cgttctgcag cgcaaagtgg
     3061 cggcggaaga tttcggcgac ttcccacgcc ccccacggtt ccctgggccg cacgccgaca
     3121 ttcaggaaga gcgacccttg cggggccagg cagcgcttgg cctccgcggc ccagcggtcg
     3181 gtccaggcca gatattcctg acgcggcagg tcatcattgt actttgagta cttgatattc
     3241 agattgtagg gcggcgaggt gacgatgaca tcgattgacc catcggctat gaccgccggc
     3301 gcccgcttca gaaaatcccc gtggatcagc tcgacggtcg cctttgaagg cttgaccggt
     3361 ggcgacccct tcttcctgcg gacgggccgc tgcgcctcgg gggattcgct catctcgccg
     3421 aacagatcgg cctgccgtgc ttccttcatc gtaagcattc cccaggattg ctgaacatga
     3481 aaaggggggc cggctcgcac cacgcccccc ctgtcacctg ctcggaaatc ggttctcagc
     3541 tcacgctatt cctgggaacc gtaggtttca tgagcgactc aggactcgcc gtccgccgct
     3601 gcctcagacg atcgtcttct cggtctcgat atcgaagtaa tggcccttgg ccgcgttcat
     3661 cagcagcgcc ttttcgtcgt tcgtctcggc gcgcacgtgc gcttcgaccc gcgcgatgaa
     3721 cggatggcgg ccggtggtca ggtagagata gacctccgag cccatcggct ccaccacttc
     3781 gatccgcgcc ttcagttcgc ggtagtgcgg atcggccttc tgatagctgg ggtcgaagaa
     3841 atcctccggc cgcacgccaa acgtcacgcg cttgttgttg tggttgcgca gcgcctccac
     3901 gtgggcatcg tagaccggga agtcgatcgt gccgctctcc tcgacaaaat gcagcgtccc
     3961 gccgcgctcg accagttcgc cgtcgatgaa gttcatcggc ggggagccga tgaaccccgc
     4021 cacgaacttg ttggcgggat tgtcgtacag atcaatcggc tcggcgatct gctggatccg
     4081 cgcgtcattc atcacgacga tccggtcgcc catcgtcatc gcttcgacct gatcgtgcgt
     4141 cacgtaaatc atcgtcgcct gcagccggtg atgcagcttg ctgatctccg tccgcatctg
     4201 cacgcgcatc ttcgcatcga ggttcgacag cggctcgtcg aacaggaaca cctgcggctt
     4261 gcgcacgatg gcgcggccga ccgccacgcg ctggcgctga ccgcccgaca gcgccttggg
     4321 cttgcggtcc agatactccg tgatcccgag gatctcggcc gcctcgcgca cgcgccggtc
     4381 gatctccgcc ttcgggaact tgcgcagcat cagcccgaac gccatgttct tatacaccgt
     4441 catgtgcggg tagagcgcgt agttctgaaa caccatcgcg atgtcgcgat ccttgggcgg
     4501 cacgtcgttg accagccggt cgccgatgta aatctccccc gcggtgatct cttccagccc
     4561 cgcaatcatc cgcagcgtgg tggatttgcc gcacccggag gggccgacca ggaccacgaa
     4621 ttccttgtcc tggatctcca gcgagacctg ctccacggcg ggcttgggca cgccgggata
     4681 gactttcttg acgttcttga gcacaacttg cgccatcgaa ccgaccctgc cttttcaaaa
     4741 aacccgcccc ggcttcgatc aaccacagag gcgcgactcc accttttccc ccagccgttc
     4801 aaccatgttg cgtggaatcg ctgacccgga cacccgtgac ccgaacccga ggtcggcctc
     4861 caattatgcg gcgcaagtcg aaattcgcaa gcaggaacac cgccctcccg gcacccgaca
     4921 tacaaggatg caggcccgga caaggggaaa ctggcccggg cggcctagcg gcccaattca
     4981 tcccatccgc agggctcgcc gccgtgccgg tccccgattt tcgacccgtg aacagccagg
     5041 gccgccaacc ggggcgatat ttcttgaaac aaatttgcgg gctgcggtgt cttaacctgc
     5101 gggagcgtcc tgcccatccg ggccggatcg cttccccaac tcaatgcgcg cggatttcag
     5161 gttctaattt gacacggagc at
//
LOCUS       DAOPCO010000285         5169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13640_length_5169_cov_5.965194, whole genome shotgun sequence.
ACCESSION   DAOPCO010000285 DAOPCO010000000
VERSION     DAOPCO010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13640_length_5169_cov_5.965194"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLS90_14815"
     CDS             <1..534
                     /locus_tag="PLS90_14815"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPK03718.1"
                     /translation="PLDLELDLDPRQLQLVDEEFDFTDRVRGRLTFRMAGADILAQGD
                     LTARIMGRCVRCLGPAAAEIHVPVTEVWMHGPGRDERGIAADADDLDLTRFYTGEEIE
                     LAEPLRELIMAELPDRLYCDPDCKGLCPGCGANLNTEPCACSPDVRRQREAGREPDWK
                     QALKNLHLGDSGGSERT"
     gene            651..1565
                     /locus_tag="PLS90_14820"
     CDS             651..1565
                     /locus_tag="PLS90_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03719.1"
                     /translation="MNTRSRLRPRSGALRWVLPLILPALLNSCAWLGGRPDFAYPETL
                     TRQPFAQTLTDPRHYKEAYREVFPFLPKVHMMGRLSASSGWWPGQRHFDFSFFGLDRN
                     QLRLGGFHRAAGIRLFDVMVVDNIMTVTFHEGGQQGHVFQGPIPPEGSPFLARYGVEP
                     GDLMPIFQIGSQITGSRFRVTVSGRDWILEPLELLAEAPGLRRILLDARSGLPRRIDW
                     ETDNRAWQVWYTRWDYFTDEQIGETRLMPQEVLIEGQRPRTRIRLDITEDSYQFGTRV
                     PDRIFQVDVPYRYLFHPLNELDEVFDTE"
     gene            1562..4180
                     /locus_tag="PLS90_14825"
     CDS             1562..4180
                     /locus_tag="PLS90_14825"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPK03720.1"
                     /translation="MRRRFLRFLAITTFRHAWFFFPLLALLTAGAVHTLRRMEVDTSL
                     VAFLPPASPEALNIREVVRDYRNLEPIVVTVRSREPGHEAVLMSVATELARWLDDARY
                     FLPPIYRVDELAQDYYSSLSDLRLIQLLTPEDWAELRDADNLARHLKRLAAHRLNAFA
                     PAEVRRARTLREDPLGALEAIQRRVAISRGPTRLETRDGYFMSPDGRAIYLLLYPVVS
                     AENGRNATRILWFLQSSRRSILERRAPWADLVEIDFAGNHVTTARQIESVQDDFDKIL
                     KITVPLAFLLVLLVFHKLESLFFIFLPPAIGLVWTLALATLAFNGISAVMAAFLIVIS
                     AMGIGYSIHMYHRFTLELYQKRNYYRALSRSYVETGRGVLASAVIVTVLFALLFVTSL
                     RDVQHWRDLSRILRDSQGLAQFGIIAAIAILCNMLACLIVIPLFASLKALLARGRVKP
                     VQLYRFSLERFYEPAVTRPRMTLGVMLLLSVFLGYQARNLDFFPRFASISAFFYPADE
                     QLGEADNRFGRPGRPVIAVVRGDTLQRALEQNDQLYRNLNRLDRDVYGILSYDSLRIA
                     LPSIRSQQEALNELRRIDLARLRDELQAAAGDLKLAPGVFQSYLEQLRQLQLRSDNPD
                     FLRFSANESDALKNTVWRYLTSREDPDGKRQYYVATMIYPRAGGFPPDRLDAMTAALR
                     VDLDQLELIGDPFVERELAQLIKYNLAIMILMAVLCILLGLILHFRSSRLAWLVFIPV
                     VAEIVWICGLMALANLRIHFFTVLALPLILSLVLDNALQLTQYHHDRRPCSVRHAMLA
                     VGRVIPLTAGIMALCYGTFALMRHPGFRQFGLTVLLGSAAVLVGSAMLLPALLQLLGR
                     GQSLGVVLRVDSETEI"
     gene            4263..>5169
                     /gene="mtnA"
                     /locus_tag="PLS90_14830"
     CDS             4263..>5169
                     /gene="mtnA"
                     /locus_tag="PLS90_14830"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPK03721.1"
                     /translation="MLVNNQPYRTVWLEGSTVRLIDQRLLPHRFEIVGMPRHEETARA
                     IREMIVRGAPAIGAAGAFGLVQVVLEAPDGPGRDAYIDAGEALLRATRPTAQNLFYAL
                     DRVRRAFDGLPAAQQADAARAEAEALADDDVRQNEAIGRHGAALLRDGDTLLTHCNAG
                     WLATVDWGTALAPVYVARRAGRRIHVLADETRPRCQGANLTAWELLQEGVTVDVLADN
                     AAGLLMRRGEVNIVITGADRIAANGDAANKIGTYEKALLAREHGIPFYIAAPSTTFDL
                     NCPDGDAIPIEERGADEVHYAWGWTD"
BASE COUNT          795 a         2105 c         1365 g          904 t
ORIGIN      
        1 cccctcgatc tggagctgga cctcgacccg cgccagttgc aactcgtcga cgaggaattc
       61 gacttcaccg atcgcgtccg cggccgcctg acctttcgca tggccggcgc cgacatcctc
      121 gcccagggcg atctgaccgc ccggatcatg ggccgctgcg tccgctgcct cgggcccgcc
      181 gccgcggaaa tccacgtccc ggtgacggag gtctggatgc acggccccgg ccgcgatgag
      241 cgcggaattg ccgccgacgc cgacgacctc gacctgacgc gcttctacac cggcgaggaa
      301 attgagctgg ccgagccgct gcgcgaactg atcatggccg aactccccga ccggctctat
      361 tgcgaccccg actgcaaggg cctctgcccc ggctgcggcg ccaacctcaa tacggagccc
      421 tgcgcctgct cgcccgacgt ccgccgccag cgcgaagccg gccgcgagcc cgattggaaa
      481 caggcgctca agaacctcca tctgggcgat tccggaggat cggaacgaac ctagcggacc
      541 acggatcgga ttgccttcca catcttctta tgtcacttat gtcctttgcg tcccttgtgt
      601 cccttctgaa acacggattc gattacctct ccatggaagg ccccctccac atgaacacac
      661 ggtcccgcct gcggccccgc tccggcgcgt tgcgctgggt cctgccgctg atcctcccgg
      721 cgctgctcaa cagctgcgcc tggctgggcg gcaggccgga cttcgcctac cccgaaaccc
      781 tcacccgcca gcccttcgcc cagaccctga ccgaccccag gcactacaag gaggcctacc
      841 gcgaggtctt ccccttcctg ccgaaggtcc acatgatggg tcggctctcg gccagcagcg
      901 gctggtggcc cggccagcgt cacttcgatt tctcgttctt cgggctcgac cgcaatcagt
      961 tgcgcctcgg cggcttccat cgcgccgccg gcattcgcct cttcgatgtc atggtcgtcg
     1021 acaacatcat gaccgtcacg ttccacgagg gcggccagca gggccacgtc ttccagggac
     1081 cgatcccccc cgaaggctcc cccttcctcg cgcgctacgg cgtcgagccg ggcgacctga
     1141 tgcccatctt ccaaatcggc tcgcaaatca ccggcagccg cttccgcgtc accgtgtccg
     1201 gcagggactg gatcctcgag ccgctcgaac tgctcgccga ggcccccggc ctgcgccgga
     1261 tcctcctcga cgcccgcagc ggcctccccc gccggatcga ctgggaaacc gacaatcgcg
     1321 cctggcaggt ctggtatacg cgctgggact acttcaccga tgaacagatc ggcgaaaccc
     1381 ggctgatgcc ccaggaagtc ctcatcgagg ggcaacggcc ccggacccgc attcgcctcg
     1441 atatcaccga ggacagctat cagttcggca cccgcgtccc cgaccggatt ttccaggtcg
     1501 acgtccccta ccgctatctg ttccatcccc tcaacgagct cgatgaggtc ttcgacacgg
     1561 aatgagacgg cgcttcctcc gcttcctggc catcaccaca ttccgccacg cctggttctt
     1621 cttcccgctg ctggccctgt tgaccgcggg ggcggtccac accctgcgcc gcatggaggt
     1681 cgacaccagt ctggtcgcct tcctcccccc cgcctccccc gaagccctca atatccgcga
     1741 ggtcgtgcgc gactaccgca acctcgaacc gatcgtcgtc accgttcgct ccagggagcc
     1801 cggccacgag gccgtcctga tgagcgtcgc caccgaactg gcccgctggc tcgacgatgc
     1861 gcgctacttc ctccccccga tctaccgcgt cgacgaactc gcccaggatt actactcctc
     1921 cctctccgat ctgcgcctga tccagttgct gacccccgag gactgggcgg agctgcgcga
     1981 cgccgacaac ctcgcgcgcc acctcaagcg gctggcggcc catcgcctca acgccttcgc
     2041 ccccgccgaa gtccggcgcg cccgcacgct ccgcgaagac cccctcggcg ccctcgaggc
     2101 catccagcgg cgcgtcgcca tcagccgcgg tccgacccgg ctcgaaaccc gcgacggcta
     2161 cttcatgagc cccgacggcc gcgccatcta cctcctcctt taccccgtcg tctcggccga
     2221 aaacggccgc aacgccaccc gcatcctctg gttcctgcaa agcagccgcc gctcgatcct
     2281 ggaacggcgc gccccctggg ccgacctcgt cgagatcgat ttcgccggca atcacgtcac
     2341 caccgcccgc cagatcgaaa gcgtccagga cgacttcgac aagatcctca agatcaccgt
     2401 tcccctcgcc ttcctcctcg tcctgctcgt ctttcacaag ctggagtcgc tcttcttcat
     2461 cttcctcccc cccgccatcg gcctggtctg gaccctcgcc ctggccacgc tcgccttcaa
     2521 cggcatctcc gccgtcatgg ccgcgttcct gatcgtcatc tcggccatgg gcatcggcta
     2581 cagcatccac atgtatcacc gcttcacgct cgaactgtat cagaagcgca attactaccg
     2641 ggcgctctcg cgctcctacg tcgaaaccgg ccggggcgtt ctcgcctcgg ccgtcattgt
     2701 cacggtcctc ttcgccctcc tgttcgtcac ctcgctgcgc gacgtgcagc attggcgcga
     2761 tctgtcgcgg atcctccgcg attcgcaggg cctcgcccag ttcggcatca tcgccgccat
     2821 cgccatcctc tgcaacatgc tcgcctgtct catcgtgatc ccgctcttcg cttcgctcaa
     2881 ggccctgctg gcgcgcggcc gcgtcaagcc cgttcagctc tatcgcttca gtctggagcg
     2941 cttctatgaa ccggccgtca cccgcccgcg catgaccctc ggcgtcatgc tcctgctcag
     3001 cgtcttcctc ggctaccagg cccgcaacct cgatttcttt ccgcggttcg cctcgatttc
     3061 agccttcttt tatcccgccg acgagcaact cggcgaggcc gacaaccgct tcggccggcc
     3121 cggccgcccc gtcattgccg tcgtgcgcgg cgataccctg cagcgcgccc tcgaacagaa
     3181 cgaccagctc taccgcaacc tgaaccgcct cgatcgcgac gtctacggca tcctctccta
     3241 tgactcgctg cggatcgcgc tgccgtcgat ccgctcccag caggaagcct tgaacgaatt
     3301 gcgccgcatc gatctggcgc gcctgcgcga cgagctgcag gccgccgccg gcgacctcaa
     3361 actcgccccg ggggtctttc agagctacct cgaacaactg cgccagcttc agttgcgcag
     3421 cgacaacccc gatttcctgc gcttctccgc caatgaaagc gacgcgctca aaaacaccgt
     3481 ctggcgctac ctgaccagcc gcgaggaccc cgacggcaag cgccagtact atgtcgccac
     3541 gatgatctac ccccgcgccg gcggcttccc ccccgaccgg ctcgacgcaa tgaccgcagc
     3601 gctccgcgtc gacctcgatc agctcgaact gatcggcgac cccttcgtcg agcgcgagct
     3661 cgcccagctc atcaaataca acctcgcgat catgatcctg atggccgtcc tctgcatcct
     3721 ccttggcctg attcttcact tccgcagttc acggctggcg tggctcgtct tcatccccgt
     3781 cgtcgccgaa atcgtctgga tctgcggcct gatggcgctc gccaacctgc gcatccattt
     3841 cttcaccgtc ctcgccctgc cgcttatcct cagcctcgtc ctcgacaacg ccctgcagct
     3901 gacgcagtat catcacgacc ggcgcccctg ctccgtccgc cacgcgatgc tcgcggtcgg
     3961 ccgcgtcatc ccgctcaccg ccggcatcat ggccctgtgc tatggcacgt tcgcgctgat
     4021 gcgccatccc ggcttccgcc agttcggcct caccgtcctg ctcgggtcgg ccgccgtcct
     4081 cgtcggcagc gcgatgctgc tccccgccct gctccaactc ctcggccgcg gccagtcgct
     4141 cggcgtcgtc ctccgcgtcg acagcgaaac cgaaatctga ttcccgaccc acgatgaaca
     4201 ccccacttgg ccttcttccg tcccttatgt cccttacgtc ccttcccagg aagcactgaa
     4261 ccatgctcgt caacaatcaa ccctaccgca ccgtctggct cgaaggttca accgtccgcc
     4321 tgatcgatca gcggctgctc ccccaccgct tcgagatcgt cggcatgccg cgccacgagg
     4381 agaccgcccg cgcgatccgc gagatgatcg tccgcggcgc ccccgccatc ggcgccgccg
     4441 gcgccttcgg cctcgttcag gtcgtcctcg aagcccccga tggccccggc cgcgacgcat
     4501 acatcgacgc cggcgaggcc ctcctgcgcg cgacccgccc cacggcgcag aatctgttct
     4561 acgccctcga ccgcgttcgc cgcgccttcg acggcttgcc cgccgcgcag caggccgatg
     4621 ccgcccgcgc cgaggccgaa gccctcgccg acgacgatgt ccgccagaat gaggccatcg
     4681 gccgccacgg cgccgccctc ctccgcgatg gcgacacgct gctcacccac tgcaacgccg
     4741 gctggctcgc caccgtcgat tggggcacgg ccctcgcccc ggtctatgtc gcccgccgcg
     4801 ccggccgccg catccatgtc ctggccgacg aaacccgccc ccgctgccag ggcgccaacc
     4861 tcaccgcctg ggaattgctc caggaaggcg tcaccgtcga cgtcctcgcc gataacgccg
     4921 ccggcttgct catgcgccgc ggcgaggtca acatcgtcat caccggcgcc gaccgcatcg
     4981 ccgccaacgg cgacgccgcc aacaagatcg gcacctacga aaaagccctc ctcgcccgcg
     5041 aacacggcat cccgttctac atcgccgccc cctccaccac attcgatctc aactgccccg
     5101 acggcgacgc catcccgatc gaagaacgcg gcgccgacga ggtccactat gcctggggct
     5161 ggaccgacg
//
LOCUS       DAOPCO010000286         5131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13763_length_5131_cov_6.119385, whole genome shotgun sequence.
ACCESSION   DAOPCO010000286 DAOPCO010000000
VERSION     DAOPCO010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13763_length_5131_cov_6.119385"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1232)
                     /gene="glk"
                     /locus_tag="PLS90_14835"
     CDS             complement(228..1232)
                     /gene="glk"
                     /locus_tag="PLS90_14835"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPK03722.1"
                     /translation="MSGKPCLLAGDVGGTKVNVACFDPDGDLLTPLAEASYATADVAG
                     LADIARRLLGDAVGSVAAACFGVAGAVIDRRAQMVNVPWPADARDLERLFGHDRVDLI
                     NDLVALAYGVLTLRPRDLAVLHAGDPLPGGNKAVIAAGTGLGESMLFHHAGRYYPSPS
                     EGGHADFSPNCDEEIDLLRYMMDRIGRTSTERLLSGPGLVRIHEFLRDTGREEEPDWL
                     AERLRRGDASAEISQAALERRAPICERALDLFVRIYGAEAGDLALTVLATGGVYLGGG
                     IAPKILPRLREGPFMEAFHHKGRLSEMMARIPVHVVLEPRAPLFGAAWRARELLRPVG
                     "
     gene            complement(1351..2163)
                     /locus_tag="PLS90_14840"
     CDS             complement(1351..2163)
                     /locus_tag="PLS90_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPK03723.1"
                     /translation="MPAAPPATPIVALLTDFGVGDWYVGAMKGEILRRAPHAHLVDVT
                     HGLPPHQVLPGAFVLHCAAGSFPAGTIFCCVVDPGVGTERRALCGRVGPWLFSGPDNG
                     LLSGLLEGLAPDDLDLHRIENPEFRNERVSSTFHGRDLFAPAAARLALGFDPAQAGPP
                     VADPLRLPLFELAEQADRLTGRVVLIDQFGNLVTNIRRRSHGAWFEPGRFVIHAGALR
                     LDTLSTTFGEVRHVQALAYWGSAGTLEIAVNQGSAAGLTGLRLGDAIDVERR"
     gene            complement(2199..4874)
                     /locus_tag="PLS90_14845"
     CDS             complement(2199..4874)
                     /locus_tag="PLS90_14845"
                     /inference="COORDINATES: protein motif:HMM:NF023636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPK03724.1"
                     /translation="MNPVTESGKTTKGWPLRKLLQRGAPIVYRGRQLDEIAMPVGGIG
                     TGSISFGGWGQLRDFEIFNKPDKGLELQYTFFTLYARQAGHEPITRVLQGPVGGNEFM
                     GPNRGNAYHSYGFGMNRLSGAGLPHFRQGEFIGMFPFGRVDLRDEKMPVAASVEAWNP
                     FIPRNADDSGLPAALFNIELRNTTPDPVDVTLIANLENRIGHPEPGGGTIEVFEEPTV
                     RGLSMTTRRHKPGSPRYGTMALATPHKDVSYQTAWTRGGWFDAFHRFWDHATQGRLQE
                     NREPTVREPDDRGTDIGSIALHARIAPGTSIRLPVWIIWHNPVFEMNWAQRCECGPGG
                     DCTPYPIWRNYYATVHRDALAVARYLGEHHVRLERETRAFAEALYGSTLPAAVMDAVG
                     SQISILKSTTCIRLEDGTFYGFEGCFPGMGCCEGTCTHVWNYAQALPFLFPALERSVR
                     EADFEHRLSEDGHMVFRMPLPLGTPPQATGHAAADGQMGGVLKFYREWVQTGDDDWLR
                     RHWPSLVRSLEYAWRFWDQDKDGVMEGVQHNTYDIEFYGPNTMMGSLYHGALRAAEIM
                     ARHLGEHDKAREYRRLREQGRAWMDQHLFNGEYYIQEIRPDAPSSHERALLGWQGEDP
                     DKPEIPRYQYGKGCLSDQLLGQWFARVVGLGQLFDPKHVKSALDSIFRYNWRETLVEH
                     ANPQRIYAVGDEAGLLLATWPHGERPGLPFVYSDEVWTGIEYQVAAHLIYEGRLREGL
                     SIVKGLRDRFTGERRNPFNEFECGNHYARALASYSLLLALSDFQYSAPERRLAFAPRV
                     YADDFACFFSIDPAWGMLRQRAAQRGGAAEVEVKDGRLVLAEIAVGLKADAAEVELAG
                     RKVPAQAQAERKRLVLRFAEPVTIETGQTLRIVAG"
BASE COUNT          822 a         1739 c         1767 g          803 t
ORIGIN      
        1 gcgccacctt gccaaggtgg atgtcgccgg ttcgagtccg gtttcccgct ccatttaagt
       61 ttatcagaat caaagtcttg caagaagaag ccgccgggtc acgaccggcg gcttttcttc
      121 tgggtcgcgc gtcgatactt cggctgtttt gcgggggccg agacggaagg ttccgtccgg
      181 ggagcatgcg ttgaaaaact ctggagtgtc tgccgaatgg aggaaattta tccgacaggc
      241 ctcagcagtt cgcgggcgcg ccaggcggcg ccgaacagcg gcgcgcgcgg ctccagcacg
      301 acgtggaccg ggatgcgcgc catcatctcg ctgagccgcc ccttgtgatg gaatgcctcc
      361 atgaacggcc cctcgcgcag ccgcggcagg atcttggggg cgatgccgcc gcccaggtag
      421 acgccgccgg tggccagcac ggtcagggcc agatcgcccg cttcggcgcc gtagatccgc
      481 acgaacagat ccagggcgcg ctcgcagatc ggcgcgcgcc gttccagcgc cgcctgcgag
      541 atctcggccg acgcatcgcc ccggcgcagc cgttcggcga gccagtccgg ctcttcctcc
      601 cgtcccgtgt cgcgcaggaa ctcatggatc cggaccaggc ccgggccgct cagcaggcgc
      661 tcggtgctgg tgcggccgat gcggtccatc atgtagcgca gcaggtcgat ttcctcgtcg
      721 cagttggggg agaaatcggc gtggccgcct tcggacggcg aggggtagta gcgcccggcg
      781 tggtggaaca gcatcgattc gcccaggccg gttcccgccg cgatgacggc cttgttgccg
      841 ccggggagcg ggtcgccggc atggagcacg gccagatcgc gcggccggag cgtcagcacg
      901 ccgtaggcga gggcgaccag gtcgttgatc agatcgacgc ggtcgtgccc gaacaggcgt
      961 tccaggtcgc gcgcatcggc cggccagggg acgttgacca tctgggcgcg gcggtcgatg
     1021 accgcgccgg ccacgccgaa acaggcggcc gcgaccgagc cgaccgcgtc gccgaggagg
     1081 cggcgcgcaa tatccgccaa cccggcgacg tcggccgtcg cgtagctggc ctcggcaagc
     1141 ggggtcagca ggtcgccgtc gggatcaaag caggcgacgt tgaccttcgt gccgccgaca
     1201 tcgccggcca ggagacaggg tttgccgctc atcgggccgt cccaggcaag aattcgtccc
     1261 tcacgccaaa cggaaaggac atcagggact taagggacat gagagacggc gcttttgcaa
     1321 gtggcttcgc cttcgctgga agccccgtgc ctagcgccgc tcgacgtcaa tggcgtcgcc
     1381 cagtctgaga ccggtcaggc cggcggccga gccctggttg acggcgatct ccagcgtgcc
     1441 ggccgagccc caataggcca gcgcctgcac gtggcgcacc tcgccgaacg tggtgctgag
     1501 cgtatcgagc cgcagcgccc cggcatggat cacaaagcgg cccggttcaa accacgcacc
     1561 gtgggaccgg cgccggatgt tcgtcaccag attgccgaat tgatcgatca gcacgacccg
     1621 gccggtcagc cggtcggctt gctccgccag ttcgaagagc ggcaggcgca gcgggtcggc
     1681 gaccggcggg ccggcctgag ccggatcgaa ccccagcgcg agccgcgccg cggcgggggc
     1741 gaacagatcg cgcccatgga acgtcgagct gacgcgctcg ttgcggaatt cggggttttc
     1801 gatccggtgc agatccagat cgtccggcgc cagaccctcc agcaaccccg acagcaggcc
     1861 gttatccggg ccggaaaaga gccacggccc gacgcggccg cagagggcgc gccgctcggt
     1921 gccgacgccg gggtcgacga cgcagcagaa aatcgtgccg gcggggaacg accccgccgc
     1981 gcagtggagc acgaacgcgc cgggcaggac ctggtgcggc gggaggccgt gcgtcacgtc
     2041 gacgagatgg gcgtgcggcg cgcggcgcag gatttcgccc ttcatcgcgc cgacatacca
     2101 gtcgccaaca ccgaagtcgg tcagcagcgc gacgatgggc gtcgccgggg gcgcagcggg
     2161 catcgatcga ctccggtgcg gacgcggccg gccgcggttc agccggcgac aatccgcagg
     2221 gtctggcctg tttcaatcgt aaccggttcg gcgaaccgca gcacgaggcg cttccgttcg
     2281 gcctgggcct gggccggcac cttccggccc gccagctcca cctcggcggc gtcggccttc
     2341 aggcccacgg cgatctcggc cagcacgagc cgcccgtcct tgacttcgac ctcggcggca
     2401 ccgccgcgtt gggcggcccg ctggcgcagc atgccccagg ccgggtcgat cgagaagaag
     2461 caggcgaaat catcggcata gacgcgcggg gcgaacgcca gccgccgctc cggcgcgctg
     2521 tactggaagt cggacagcgc gagcaggagc gaatacgacg ccagcgcgcg ggcgtagtga
     2581 ttgccgcact cgaattcgtt gaacgggttg cggcgctcgc cggtgaagcg gtcgcgcagc
     2641 cccttgacga tcgacagccc ctcgcgcagc cggccctcgt agatcagatg cgcggcgacc
     2701 tgatattcga tcccggtcca gacctcgtcg ctgtagacga acggcaggcc gggccgttcg
     2761 ccgtggggcc atgtcgccag cagcaggccc gcttcgtcgc cgacggcgta gatccgctgc
     2821 gggttggcgt gctccaccag cgtttcgcgc cagttatagc ggaagatcga atccagcgcg
     2881 gacttgacat gcttcgggtc gaacagttga cccaacccga ccacccgcgc gaaccattgc
     2941 cccagcagtt ggtccgagag gcaacccttg ccgtactggt agcgggggat ttcgggtttg
     3001 tccggatctt cgccctgcca gccgagaagc gcgcgctcgt gcgagctggg cgcatcgggc
     3061 cggatttcct ggatgtaata ctcgccgttg aacaggtgct gatccatcca ggcgcgcccc
     3121 tgctcgcgca gccggcggta ttcccgggcc ttgtcgtgct cgcccaggtg gcgcgccatg
     3181 atctcggccg cgcgcagcgc gccgtgatac aggctgccca tcatcgtgtt ggggccgtag
     3241 aactcgatgt cgtaggtgtt gtgctgcacc ccctccatca cgccgtcctt gtcctgatcc
     3301 cagaagcgcc aggcgtactc cagcgaacgg acgaggctgg gccagtggcg gcgcagccag
     3361 tcgtcatcgc cagtctgcac ccactcgcga tagaacttga gcacgccgcc catctggccg
     3421 tcggcggcgg catggccggt cgcttggggc ggcgtcccca gcggcagcgg catgcggaac
     3481 accatgtggc cgtcctcgct caggcggtgc tcgaaatcgg cctcgcgcac gctgcgttcg
     3541 agcgcgggga acaggaacgg gagcgcctgg gcatagttcc agacgtgggt gcaggtcccc
     3601 tcgcagcacc ccatgccggg gaaacagccc tcgaaaccgt agaacgtgcc gtcctccagc
     3661 cggatgcagg tggtcgactt gaggatcgag atctgcgagc cgacggcgtc catgacggcg
     3721 gccggcagcg tggagccgta gagcgcctcg gcaaacgccc gcgtctcgcg ttccagccgc
     3781 acgtgatgct cgcccaggta gcgcgcgacg gcgagcgcat cgcggtggac cgtggcgtag
     3841 taattgcgcc agatcggata cggcgtgcag tcgccgcccg gcccgcactc gcagcgctgc
     3901 gcccagttca tttcgaacac cgggttgtgc cagatgatcc agaccggcag gcggatgctt
     3961 gtccccggcg cgatgcgggc gtgcagcgcg atcgagccga tgtcggtgcc gcggtcgtcg
     4021 ggctcgcgca cggtcggctc gcgattctcc tgcaggcggc cctgcgtcgc atggtcccag
     4081 aagcggtgga aggcatcgaa ccagcccccg cgcgtccagg cggtctggta gctgacgtcc
     4141 ttgtgcggcg tcgccagcgc catcgtgccg tatcgcgggc tgcccggctt gtggcgccgc
     4201 gtcgtcatgc tcagcccgcg gacggtcggt tcttcgaaga cctcgatcgt gccgccgccg
     4261 ggctcgggat ggccgatccg gttttccaga ttggcgatca gcgtgacatc gacggggtcg
     4321 ggcgtcgtgt tgcgcagctc gatgttgaac agcgcggcgg gcaggcccga gtcgtcggcg
     4381 ttgcgcggga taaacggatt ccaggcctcg acactggccg cgacgggcat tttctcgtcg
     4441 cgcaggtcga cgcggccgaa cgggaacatg ccgatgaatt caccctgccg gaaatgcggc
     4501 aggcccgccc cgctgagccg gttcatgccg aacccatacg agtggtaggc gttgccgcgg
     4561 ttcggcccca tgaattcgtt gcccccgacg gggccttgca ggacgcgcgt gatcggctcg
     4621 tggcccgcct ggcgtgcgta cagcgtgaaa aacgtatact ggagttccag tcccttgtcg
     4681 ggcttgttga agatttcgaa atcgcggagc tggccccagc cgccgaacga gatcgatccc
     4741 gtgccgatcc cgccgaccgg catggcaatc tcgtcgagct gccggccgcg atagacaatc
     4801 ggcgcgccgc gctgaaggag tttgcgcagc ggccagccct tggtcgtctt gcctgattcg
     4861 gtaacgggat tcatgacatc ttcctcgatt aaacgcttcg cctcccagcc ggccgcactc
     4921 gcctcccggc gaatgcgccg catgtacagc agatccctat actccgcgcc gcgcgaatct
     4981 gcaaacggga aaagctcgtc cgcggcgcgt cccgagtgca cgccgcgcgg tcctttccgc
     5041 cgccggctgc cgttgttcgt cgttcgcacg cgccccggcg ggaaaaagcc aatgaaaatc
     5101 agccgctgaa accccgaaaa cattagcccg c
//
LOCUS       DAOPCO010000287         5115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13812_length_5115_cov_5.145059, whole genome shotgun sequence.
ACCESSION   DAOPCO010000287 DAOPCO010000000
VERSION     DAOPCO010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13812_length_5115_cov_5.145059"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLS90_14850"
     CDS             <1..1084
                     /locus_tag="PLS90_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03725.1"
                     /translation="TVRHDPYFPSTAGRLIGSLVKYAAASGEERPLALARRIARHALA
                     TCFDPAGAAIEEKTGWHVHSVTSTISSLAELARADGDRELLERTEQLYKAAFGRLITR
                     IGWSKENLSNNLDIGEINNTGDIIQTALILSSTGVGDYYSDVWRMMHAHFLPAQLTDT
                     SWVNVVETPASDAERDIRGRVLGAFGFPAPYGHKSQLVDYLNFNFDITCGALQALCEV
                     QRHVVQSHNGACNVMLPFACADGKLALGPLGDEQSGWRVEISEPLSVALRLPDWAAPE
                     TIRVQSAGRDLAPVIRDRALLIQHTDGRPGGYDILIPDAEYETDERIMDVDYRVLWRG
                     DQIVSMTAGETAIPFFETPGHSAICK"
     gene            1075..2373
                     /locus_tag="PLS90_14855"
     CDS             1075..2373
                     /locus_tag="PLS90_14855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HPK03726.1"
                     /translation="MQIGRLCLLVLCVVLQGCGGGKARNAGQIVLKFSTWGSPEEMTI
                     NRRIIDAFMSENPDIYVDIMHIPSSQYNTKLQILQAARVAPDVMWLNAWNAYTLYRNN
                     RLLPLNGFIEEERKTNPEFLREPDSLVTHIESSYCYKGEYFLSPLGPVTYHLYYNKNL
                     FDEENLAYPDETWDWDMLAAAAARLSRFRGDTPIQFGIHFHNWWGAWINFVWQNDGLL
                     FDRMVDPTQCLIETAEAREALQFLHDLIYVQRAAPSPTQMSLLGGDFMTGKVAMQLQG
                     SWMTEQYRYIEDFEWDMAPLPRRKQQAVGVRVCGHGIRRDTKYPEAAWRLVRFYQTER
                     AQRLMGDFALWIPTREAWALDPEFWHPAGVPPDHARKRVEDIKRGRPGDLLDVAATRI
                     CETIIPMELDPFWLNKETLEECVARITPQVNAILAERTGN"
     gene            2377..3264
                     /locus_tag="PLS90_14860"
     CDS             2377..3264
                     /locus_tag="PLS90_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPK03727.1"
                     /translation="MGRAERRELLAGYLFAMPWLIGIVLFTAGPVIFSFIISFTDWDL
                     LNPPHWIGWANYATLVHEDPLFWKSIRVSAIYTLFSVPLGMAFALAVALALNMNLPGQ
                     SVMRTIYYLPSILSGVAVALLWQWIFNAEFGLINVLLRKIGLPAPGWLTSEQWALPAL
                     IIMSLWGIGGTMIIYLAGLQGIPPHLYEAARIDGANLLQMFRNITLPMLSPVLFFTLI
                     MGIIGSFQVFTQAYVMTNGGPAHATLFFVLYLYRNAFEWLKMGYASALAWLLFIIILL
                     LTLINFYMSRYWVHYDDAG"
     gene            3248..4165
                     /locus_tag="PLS90_14865"
     CDS             3248..4165
                     /locus_tag="PLS90_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK03728.1"
                     /translation="MTTPAEPTLSQASPGRRALAPPSWTQSARLRRRVLGLCAFIFLA
                     AGAVIILLPFGWLVSTSLKRPAEVFVSPPQWIPPDPQWSNYREVIQTWPFLRYGLNTV
                     YVTLMVMLGSLLSCSLAGYSFARLRWRGRNVIFFLSIMTMMIPAQVTMIPLFSLFVAL
                     GWVDTLLPLYVPAFFGSAFFIFLFRQFFMSIPRSLEDAARIDGCGYLGIYWHVVVPLS
                     VPSFITVAIFTFIGTWNDFMGPLIYISSESKRTLALALSFYQNYFYGGIHLHTLMAAS
                     LMVLLPCIIVFFIFQRHLIQGMILSGIKG"
     gene            4266..>5115
                     /locus_tag="PLS90_14870"
     CDS             4266..>5115
                     /locus_tag="PLS90_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03729.1"
                     /translation="MLIAAPQTPPAAEPLEINDHDLDRALLAGLHCMANITNPDDRHI
                     PYFECFVLGRPTMLFNSQLSVGNVTGRCLYAMLKAEAELGIEVDPVVIARYRQVLIDS
                     YSVVRAIPANPNYPGGPYQTCWIFNDGAGLRGLMALVNYRDDALAREILDDTVTNYKL
                     YWQDQGYSWEAFRAHFNLTGGGYGGANPWPGAGIINARRSYFRIEPWLKYHLVQGAPA
                     AFALASSLGNIMLSYNFPASGALVGYDHMFEPVAEMNNMALLATITNNAAMMNRVRAR
                     YDNGVRA"
BASE COUNT          864 a         1618 c         1603 g         1030 t
ORIGIN      
        1 cacggttcgg catgacccgt acttcccctc cacggccggg cgcctgatcg gctcgctggt
       61 gaagtatgcc gcggccagcg gcgaggagcg gccactggcg ctggcgcgcc gcatcgcgcg
      121 ccacgcgctg gcgacctgtt tcgatcccgc gggcgcggcg atcgaggaga agaccggctg
      181 gcacgtccac tcggtcacct cgacgatttc gtcgctggcc gaactggccc gggccgacgg
      241 cgaccgcgag ttgctggagc ggaccgagca actctacaag gcggccttcg gccggctgat
      301 cacgcgcatc ggctggtcga aagagaatct gtcgaacaat ctcgacatcg gcgaaatcaa
      361 caacaccggc gacatcattc agaccgcgct gatcctgagc agcaccggcg tgggcgatta
      421 ttacagcgac gtctggcgga tgatgcacgc gcacttcctg ccggcgcagt tgaccgacac
      481 cagttgggtc aacgtggtgg agacgccggc cagcgatgcc gagcgcgaca tccgcggacg
      541 cgtgctgggc gcattcggtt tccccgcgcc gtacgggcac aagtcgcagc tcgtcgatta
      601 cctcaatttc aacttcgaca tcacctgcgg cgcgctgcag gcgctctgcg aggtgcagcg
      661 gcacgtggtc cagagccata acggcgcgtg caatgtgatg ctgccgttcg cctgcgccga
      721 tgggaagctc gccttggggc cgctgggcga cgagcagtcg ggctggcgcg ttgagatctc
      781 ggaaccgctc agcgtcgcgc tgcggctccc cgactgggcg gcgcccgaga cgattcgcgt
      841 gcagtccgcc gggcgcgacc ttgctccggt gattcgcgat cgtgcgctgc tgattcagca
      901 tactgacggc cggccgggag ggtatgatat cctgattccc gacgccgagt atgaaacgga
      961 cgaacggatc atggacgttg actaccgtgt cttgtggcgg ggggatcaga tcgtctcgat
     1021 gacggccggc gaaacggcga tcccgttctt cgaaaccccg ggtcactccg cgatatgcaa
     1081 atagggcggc tctgcctgct ggttctgtgc gttgtcctgc aaggctgcgg aggggggaag
     1141 gcgcgcaacg ccggccagat tgtcttgaaa ttttcgacct ggggttcccc cgaggagatg
     1201 acgatcaacc ggcggatcat cgacgccttc atgtcggaga accccgacat ttacgtcgat
     1261 atcatgcaca tccccagttc gcagtacaat accaaactgc aaattctgca ggcggcccgg
     1321 gtggctcccg acgtgatgtg gctcaatgcc tggaacgcct acacgctcta ccgcaacaac
     1381 cggttgctgc cgctcaacgg ctttatcgag gaggagcgca agacgaatcc ggagttcctg
     1441 cgcgaacccg attcgctcgt gacccatatc gaatcctcgt actgttacaa aggggaatat
     1501 tttctctcgc cgctggggcc ggtgacctat cacctctact acaacaagaa tctgttcgat
     1561 gaagagaacc tggcctatcc cgacgaaacg tgggactggg acatgctggc ggcggcggcg
     1621 gcgcggctga gccggttccg cggcgacacg ccgatccagt tcggcatcca tttccacaac
     1681 tggtgggggg cgtggatcaa tttcgtctgg cagaacgacg gcctgctgtt cgaccggatg
     1741 gtcgacccca cgcaatgcct gatcgaaacc gcggaggccc gcgaagcgct gcagttcctc
     1801 cacgatctga tctatgtgca gcgtgcggcc ccctcgccca cgcagatgtc gctgctcggc
     1861 ggcgacttca tgacgggcaa ggtcgcgatg cagttgcagg gctcctggat gaccgaacag
     1921 tatcgctaca tcgaggactt cgaatgggac atggcgccgc tgccgcgccg caagcagcag
     1981 gcggtgggcg tccgggtctg cggccatggg atccgccggg atacgaaata ccccgaggct
     2041 gcctggcgac tggtgcggtt ctaccagacc gaacgcgccc agcggctgat gggcgatttc
     2101 gcgctgtgga ttcccacacg cgaagcgtgg gcgctcgatc cggaattctg gcaccccgcg
     2161 ggcgtgcctc ccgaccacgc ccgcaagcgg gtcgaggaca tcaagcgcgg gcgaccgggc
     2221 gacctgctcg acgtcgccgc cacgcgcatc tgcgagacga tcatcccgat ggagctcgat
     2281 ccgttctggc tgaacaagga gacgctggag gagtgcgtgg cgcggatcac gccgcaagtc
     2341 aacgccattc tggccgagcg gacggggaac tgaacgatgg gtcgggcgga acgcagggaa
     2401 ctgctggcgg gctacctgtt cgcgatgccg tggctgatcg gcatcgtgct gttcacagcc
     2461 gggccggtca tcttttcgtt catcatcagc ttcaccgact gggatctgct caatccgccg
     2521 cactggatcg gctgggccaa ttacgcaacg ctggtgcatg aggacccgct gttctggaag
     2581 tcgattcgcg tctcggcgat ctatactttg ttcagcgtcc ccctgggaat ggccttcgcc
     2641 ctggccgttg cgctggcgct caacatgaat ctgccgggcc agagcgtcat gcgcacgata
     2701 tactacctgc cctcgattct ttcgggcgtc gccgtcgcgc tgctctggca atggatattc
     2761 aatgcggaat tcggcctgat caatgtcctg ctgcgcaaga tcggcctgcc ggcgccggga
     2821 tggctgacga gcgaacaatg ggcgctgccc gcgctgatca tcatgagcct gtggggcatc
     2881 ggcggcacga tgatcattta cctggccggg ctccagggca tcccgccgca tttgtacgaa
     2941 gccgcccgga tcgacggggc caatctgttg cagatgttcc gcaacatcac gctgccgatg
     3001 ctctcgcccg tccttttctt cacgctcatc atgggcatca ttggttcgtt tcaggtcttc
     3061 acgcaggcat acgtgatgac caacggcggg ccggcgcacg ccacgctctt tttcgtgctc
     3121 tatctgtatc gcaatgcgtt cgagtggctg aagatggggt atgcctcggc gctggcctgg
     3181 ctgctgttta tcattattct gctgctgaca ttgatcaatt tttacatgtc gcgctactgg
     3241 gtgcattatg acgacgccgg ctgagcccac cctgtcgcag gcctcgcccg gccgccgcgc
     3301 gctggcgccc ccctcctgga cgcagtcggc gcgtctgcgc cgccgcgtgc tggggctgtg
     3361 cgcgttcatt tttctggcgg cgggggcagt gatcatcctg ttgccgttcg gctggctcgt
     3421 gtcgacgtcg ctgaagcgtc ccgccgaggt gtttgtttcg ccgccgcagt ggatcccgcc
     3481 cgatccccaa tggtccaact atcgcgaagt gatccagacg tggccgttcc tgcgctacgg
     3541 actgaacacc gtctacgtga cgctgatggt gatgctcggc agcctgctct cctgctcgct
     3601 ggccggctac agttttgccc ggctgcgctg gcgcggacgc aatgtcattt tcttcctgtc
     3661 gatcatgacg atgatgatcc cggcgcaggt gacgatgatt ccgctgttct cgctgttcgt
     3721 cgcgctcggc tgggtcgata cgctgctgcc gctgtatgtt cccgcgtttt tcggcagcgc
     3781 gttcttcatc ttcctcttcc ggcagttctt catgtcgatt ccacgcagcc tggaggatgc
     3841 cgcgcgcatc gatggctgcg gctacctcgg catttactgg cacgtcgttg tgccgctctc
     3901 cgtcccgtcg ttcatcacgg tcgcgatttt cacctttatc ggcacgtgga acgactttat
     3961 ggggccgctg atctatattt cgtcggaaag caagcgcacg ctggccctgg cgctgagttt
     4021 ctatcagaat tatttctatg gcgggattca cctgcatacg ctgatggcgg cgtcgctgat
     4081 ggtgttgctc ccctgcatca tcgtcttttt catttttcag cgccatctga tacagggcat
     4141 gatcctcagc ggaatcaagg gatgaggatg gaccgatgcg gccaacgggg aaacgcgttc
     4201 aacacggtca accgggattc cgcggaccgg ccgcccgacg cctttgcgcg ggatggatct
     4261 gcctgatgct gatcgcggcg ccccagaccc cgcccgccgc cgagccgctg gagatcaacg
     4321 accacgatct ggaccgggcg ctgctcgccg gcctccattg catggcgaac atcacgaacc
     4381 cggacgaccg gcatattccg tacttcgagt gcttcgttct gggccggccg acgatgctgt
     4441 tcaattccca gctcagcgtc ggcaatgtga ccgggcgctg cctgtatgcc atgctgaagg
     4501 ccgaggccga actggggatc gaggtcgatc cggtggtgat cgcgcgctac cggcaggtcc
     4561 tgattgattc ctattcggtc gtgcgggcca ttccagccaa ccccaattac ccgggggggc
     4621 cgtatcagac gtgttggata ttcaacgacg gcgccggcct gcgcggcctg atggcgctgg
     4681 tcaattatcg cgacgacgct ctggcccgcg aaatcctcga tgacaccgtc accaactaca
     4741 agctctactg gcaggaccag ggctatagct gggaggcctt ccgcgcccat ttcaacctga
     4801 cggggggcgg ctacggtggg gccaacccct ggccgggggc cggcatcatc aacgcccggc
     4861 ggagctattt ccgcattgaa ccgtggctca agtaccacct ggtacagggc gcgccggcgg
     4921 cgttcgccct ggcttcgtcg ctgggcaaca tcatgctgag ttacaatttc ccggccagcg
     4981 gcgcgctcgt cggctacgac cacatgttcg aacccgtggc cgagatgaac aacatggcgc
     5041 tgctggcgac gatcaccaac aacgcggcga tgatgaaccg cgtgcgcgcg cgctacgaca
     5101 acggcgttcg cgccg
//
LOCUS       DAOPCO010000288         5093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13879_length_5093_cov_4.549424, whole genome shotgun sequence.
ACCESSION   DAOPCO010000288 DAOPCO010000000
VERSION     DAOPCO010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13879_length_5093_cov_4.549424"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2545
                     /gene="alaS"
                     /locus_tag="PLS90_14875"
     CDS             <1..2545
                     /gene="alaS"
                     /locus_tag="PLS90_14875"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPK03730.1"
                     /translation="HHTFFEMLGNFSFGDYFKREAITWGWEFCTDPNWLGLPPERIFA
                     TVFGVQREGGAWEIDQVALDIWQRETGVVNGVTLLDEEENFWGPAGGTGACGACSEIK
                     FFTGPEDRLREYQRLSRSTDPADQARMRRDIVEEGDLFLEIWNLVFPEFDQQLDGSRP
                     LLKNRGIDTGAGLERTTTAVQFTRSGGRIRSQYETDLLLPMVRAVADVAGLPYPTLAD
                     GDDAEQSIRRRGLDPAVVRLAMNACADHARALTFTLSEGVVPSNEGRGYVLRRILRRA
                     ARFGLKLGITEPFLWRLVAPVVDVMGGAYPEITRHVAVVERTIRAEEERFTRTLNQGG
                     QILDDLLARVGAGGRLAGEEAYRLYDTYGYPLDLTIEAAAEKGISVDQPGFDRALDEA
                     KRRARASWKGGAGAARFDELLGDLPPEQRTRFCGYEGMACASRVVAILRDDLRVDSLK
                     PGQNGVVVLDETPFYAEAGGQVGDTGALDAIDSDLALFAVEDTQKTEDGHYLHFGEAR
                     ETIHAGQQVKARVDCARRIATMLNHSATHLMQGALKRLIGGHVTQQGSFVGPDGLRFD
                     FTHPEPVPEATLREIERIVNGQILANLPVTTQVMALEEAKATGAIAPFGEKYGAQVRV
                     IAMGDFSREFCGGTHVRNTGQIGLFLITGESSVASGIRRIEARTGLGALETVIQERQA
                     LHGLARQLSVPADGLPARIQALQDEIRQLRRQVEEAKAAQARRQMSEAAADFAEVAGI
                     RYLAREIPGADHNALAQAWDALRGQSPTNTAVLFYSVADGKVSMIVGLTDDLAPRRLK
                     AGDVLKETAAIVGGKGGGKPTLARGGGSQPENLPRAVEQFPSILQRLAG"
     gene            2845..4053
                     /locus_tag="PLS90_14880"
     CDS             2845..4053
                     /locus_tag="PLS90_14880"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HPK03731.1"
                     /translation="MLETLPMAPADPILGLTEAFKKDPNPNKINLGVGIYVDDEGLSP
                     LFRTVHKAEEKLWRGAQPKAYLPISGSVEYGRAVQKLIFSADSPLIADKRLRTAQTPG
                     GTGALRVAGDFLHTRFPKARIWFSNPTWNNHFNVFEAAGLEILEYPYFDPATHTLDWD
                     GLLAGLRAIPAGDVVLLHGCCHNPTGVDPSPAQWDEIGRVLAEHNLFPLVDLAYQGFG
                     DGVDEDAEGIRILARHLPEMLICSSFSKNFGLYNERVGALTVVAESDDAAERAFSHVK
                     AVIRANYSNPPAHGALIVKTILADDDLRAEWEAELVVIRGRVQQMRELFAATMKQKGV
                     EQDFSFMIDQKGMFSLSGLSREQVDWLKREKAVYMVGSGRVNVAGMTARNMNALCDAV
                     AEVLATVNVG"
     gene            4293..5060
                     /locus_tag="PLS90_14885"
                     /pseudo
     CDS             4293..5060
                     /locus_tag="PLS90_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT          858 a         2017 c         1418 g          800 t
ORIGIN      
        1 ccaccacacc ttcttcgaga tgctcggcaa cttctccttc ggcgactact tcaagcgcga
       61 ggcgatcacc tggggctggg aattctgcac cgaccccaac tggctcggcc tcccccccga
      121 gcgcatcttc gccaccgtct tcggcgtgca gcgcgaaggc ggcgcctggg aaatcgatca
      181 ggtcgccctc gacatctggc aacgcgaaac cggcgtcgtc aacggcgtaa ccttgctcga
      241 tgaggaagaa aacttctggg gaccggccgg cggcaccggc gcctgcggcg cctgctccga
      301 aatcaagttc ttcaccggcc ccgaggatcg gctccgcgaa taccagcgcc tctcccgatc
      361 gaccgatccc gccgatcagg cgcgcatgcg gcgcgacatc gtcgaggagg gcgatctgtt
      421 cctcgaaatc tggaatctcg tcttccccga gttcgaccag cagctcgacg gctcgcgccc
      481 cctcctcaag aaccgcggca tcgataccgg cgccggcctg gagcgcacca ccaccgccgt
      541 ccagttcacc cgcagcggcg gccgcatccg ctcccagtac gaaaccgatc tgctcctgcc
      601 gatggtccgc gccgtcgccg acgtcgccgg cctcccctac cccactctgg ccgacggcga
      661 cgacgccgaa caatccatcc gccgccgcgg tctggacccc gccgtcgtgc gtctggcgat
      721 gaacgcctgc gccgaccacg cccgcgcgct caccttcacc ctctccgagg gcgtcgtccc
      781 ctccaacgag gggcgcggct atgtcctgcg ccgcatcctg cgccgcgccg cccgtttcgg
      841 cctcaagctc ggcatcaccg agcccttcct ctggcgcctg gtcgcgccgg tcgtcgacgt
      901 catgggcggg gcctatcccg aaatcacccg ccacgtcgcc gttgttgaac gcaccatccg
      961 cgccgaggag gaacgcttca cccgcacact caaccagggc ggtcagatcc tcgacgacct
     1021 gctcgcgcgc gtcggcgccg gcggccgcct cgccggcgag gaggcctacc gcctctacga
     1081 cacctatggc tacccgctgg acctgacgat cgaagccgcc gccgaaaaag gcatcagcgt
     1141 cgatcagccg ggtttcgacc gcgccctcga cgaggccaag cgccgggccc gcgcttcatg
     1201 gaagggcggc gccggcgcgg cccgcttcga cgaactgctc ggcgacctgc cccccgaaca
     1261 gcgcacccgc ttctgcggct atgagggcat ggcgtgcgcc agccgcgtcg tcgccatcct
     1321 ccgcgacgac ctccgcgtcg actcgctcaa gcccggccag aacggcgtcg tcgtcctcga
     1381 cgaaacaccc ttctacgccg aggccggcgg ccaggtcggc gacaccggcg ccctcgacgc
     1441 catcgacagc gatctcgccc tgttcgccgt cgaggatacc cagaagaccg aggacggcca
     1501 ctacctccat ttcggcgagg cccgcgagac gatccacgcc gggcagcagg tcaaggcccg
     1561 cgtcgactgc gcccggcgca tcgccacgat gctcaaccac tccgcgaccc acctgatgca
     1621 gggcgccctc aagcgcctca tcggcggcca cgtcacgcag cagggttcct tcgtcggtcc
     1681 cgacggcctg cgcttcgact tcacccatcc cgagccggtc cccgaggcga cgctgcgcga
     1741 gatcgagcgc atcgtcaacg gccagatcct cgccaacctg ccggtcacca ctcaggtcat
     1801 ggcgctcgaa gaagccaagg ccactggcgc catcgcgccg ttcggcgaaa aatacggcgc
     1861 ccaggtccgc gtcatcgcca tgggcgactt ctcccgcgag ttctgcggcg gcacccatgt
     1921 ccgcaacacc ggccagatcg gcctcttcct catcaccggc gaatcgtcgg tcgcctcggg
     1981 catccgccgg atcgaggccc gcaccggcct cggcgcgctc gaaaccgtca tccaggaacg
     2041 ccaggccctg cacggtctgg cgcgccagct ctccgtcccg gccgacggct tgcccgcccg
     2101 catccaggcg ctgcaggacg aaatccgcca gttgcgccgc caggtcgagg aagccaaagc
     2161 cgcccaggcc cgccgccaga tgagcgaggc cgccgccgat ttcgccgagg tcgccggcat
     2221 ccgctacctc gcgcgcgaga tccccggcgc cgaccacaac gcgctggcgc aggcctggga
     2281 cgccctgcgc ggccaatccc ccaccaacac cgccgtcctg ttctattcgg tcgccgatgg
     2341 caaggtcagc atgatcgtcg gcctgaccga cgatctcgcg cccaggcggc tgaaggccgg
     2401 cgatgtgctc aaggaaaccg cggcgatcgt cggcggcaag ggcggcggta aaccgaccct
     2461 cgcccgcggc ggcggctccc aacccgaaaa cctcccccgc gccgtcgagc aattccccag
     2521 catcctccag cgcctggcgg gctgagcatc ccaaatctcg tccttcgtcc ttcgttcctc
     2581 gtctcccgtc ctcgtgatcg ctcccaaagg cccgctccag aaatccggct ctcgaacaca
     2641 gccgagttcc atctcccggc tctaatcccc ctcgcggtcc ttgaccagag gtccgcgtta
     2701 cgctgaaata cggtgggtgc gctcccgctt ccttgatgaa accttcggtg cagcccaacc
     2761 gcgagcccgc ccgcctgatg tcccctacgt cctttgtgtc ccgtatgtcc cttaccccaa
     2821 gtcgttcccc cggaggcgaa ccgcatgctt gagaccctgc cgatggcgcc ggccgacccg
     2881 atcctgggcc tgaccgaggc cttcaaaaag gaccccaacc ccaacaagat caacctcggc
     2941 gtggggatct acgtcgacga tgaaggcctc tctcccctct tccgcaccgt ccataaggcc
     3001 gaggaaaaac tctggcgcgg cgcccagccc aaggcttacc tcccgatcag cggctcggtc
     3061 gaatacggcc gcgccgtcca gaagctcatc ttctccgccg acagccccct gatcgcggac
     3121 aaacgcctgc gcacggccca gacccccggc ggcaccggcg ccctgcgtgt cgccggcgac
     3181 ttcctccaca cccgcttccc caaggcccgc atctggttca gcaaccccac gtggaacaat
     3241 catttcaatg tcttcgaggc ggccggcctg gagatcctcg aataccccta cttcgatccc
     3301 gccacccaca ccctcgattg ggacggcctg ctggccggcc tgcgcgccat ccccgccggc
     3361 gacgtcgtcc tgctccacgg ctgttgccac aatccgaccg gcgtcgatcc ctcccccgcc
     3421 cagtgggatg aaatcggccg cgtgctggcc gaacacaacc tcttcccgct tgtcgacctc
     3481 gcgtatcagg gcttcggcga cggcgtcgac gaagacgccg agggcatccg catcctcgcc
     3541 cgccacctcc cggaaatgct catctgctcg tcgttctcca agaacttcgg cctctacaac
     3601 gagcgcgtcg gcgccctgac cgtcgtggcc gagtcggacg atgccgccga gcgcgccttc
     3661 agtcacgtca aggccgtcat ccgcgccaac tactccaatc cccccgccca cggcgccctg
     3721 atcgtgaaaa cgattctggc ggatgacgac ctgcgcgccg agtgggaagc cgaactcgtc
     3781 gtcatccgcg gccgcgtcca gcagatgcgc gaactgttcg ccgccaccat gaaacagaag
     3841 ggcgtggaac aggatttctc ctttatgatc gatcagaagg ggatgttctc gctctccggc
     3901 ctgtcccgcg aacaggtcga ctggctcaag cgcgagaagg cggtctacat ggtcggctcg
     3961 ggccgcgtca acgtcgccgg catgaccgcc cgcaacatga acgccctctg cgacgccgtg
     4021 gccgaagtcc tcgccaccgt gaatgtcggc tgagtcactg aggcacgaac gagatccccc
     4081 tcgtccctcg tcctcgtgct cgctcccgaa ggccaccccc tttcgtgccc attcgcgtct
     4141 ttcgtgatct aattatcctc cgcccactcc ccccacccac ctttcgtccc tttcgtgacg
     4201 gccgttgcgc cgccgcgacc gtttcgctag gctagggcca aacccgccat ccgctcggcg
     4261 ggcgacatgt tccatgccgg agtcaccctc tcatgacgac ctcgagcgcc ccgcaggcgc
     4321 cctccgccgc ccaatccgca cccgatccct ggtttcagga tctcttcgcc gcgcgcatcg
     4381 gcggcgccca gtatggcaag agcaacgcca tctataagtt cgagaagatc aagcgggcga
     4441 aacgggcggc gctggcggcg caccccgaaa cccggctcct cgatttcggc atcggcgaga
     4501 acgacgagat ggccccccgc cccgtgcgcg atcgcctcaa gcgcgaagtc gacgaccccg
     4561 ccaaccgcgg ctacgccgac aacggcattc aggaatacaa ggacgcggcg gcgcgctaca
     4621 tggcccgcga atacggcgtc cagctcgacc ccgcgaccca gatcaatcac tgcatcggct
     4681 ccaaacccgc cctcgccctc ctccccggca tgctcatcaa ccccggcgac ttctgcatca
     4741 tgaccgtccc cggctacccc gtcgccggca cctggacccg ctatttcggt ggcgaggtat
     4801 ataacgcccc gctcgagccg gccaacaact acctggtcga tctggacgcc atcccggacg
     4861 agatccgccg ccgcgccaaa ctcatggtcc tcaattatcc caacagcccc accggcgccc
     4921 tggccgacga ggatttcttc aagcgagtca ttgcattcgc tcacgtaaac cgtctgatcg
     4981 tcgtcaacga cgccgcccac ctgccgctca cctatggccg caagccgttg agcttcctcg
     5041 ccgtcgacgg cgcgctcgag cagcgctgaa atttcacgga attccttgat cgg
//
LOCUS       DAOPCO010000289         5055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13988_length_5055_cov_3.809600, whole genome shotgun sequence.
ACCESSION   DAOPCO010000289 DAOPCO010000000
VERSION     DAOPCO010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13988_length_5055_cov_3.809600"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PLS90_14890"
     CDS             <1..1003
                     /locus_tag="PLS90_14890"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK03732.1"
                     /translation="VWLPDSMEGPTPISALIACGESPPSTDAGRASDDAPATPTVTLA
                     LNWFPEAEHGGYYAMKADRLDAAEGLNVVIKPGGPGNSPIPLVVTGQADYAVCNADDV
                     LLARAQGADVVALMAPLQVSPRCIMVHADSAIERLEDLRDVTLAMNAGKPFAAWLQAR
                     VPLDGVQVVPYSGGVAPFLLDPRYAQQAYVFSEPYLAQQQGASVRSLMLPDIGFNPYT
                     SVLITTAARLAADEAQAAAMVRAAVAGWRRYLEQPAPANALIHAANPEMSLEILQYGA
                     DVLRDLVQPASLPAEQIGTMTPQRWAELTGQLEELQLLPAGAVNAEDAYTLRFLPQP"
     gene            1574..3856
                     /locus_tag="PLS90_14895"
     CDS             1574..3856
                     /locus_tag="PLS90_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HPK03733.1"
                     /translation="MSLPPPPFPQKNQDHHADGWLLDLYPQAADMILWFILDDGRRLR
                     LRDPFTPIFYVSFEGQPDPEQSRRALRAALARLDGLEPAGPARRLDFWSGDQIDVEAI
                     RVTNLDLAGPNLRALARQLPELTCYNCDIAPEIHYAYDRGLFPTARCRLTWRGDTLTA
                     CELLDRADDTRYDLPPLREAQLTARGNLLGRRPRLLSLSLACEGRTLTWDDAPPAEML
                     RSLRDALRACDPDLLWTEGGDAALLPALLTLAQRHRAQLGPDADLALDREPGIRRRLR
                     TDGHTFVSYGQVLYQAPDYPLFGRWHIDRHNSFWAGTIGLAGLVEVARLARLPVQRAA
                     RRSIGTGISSIQLHHAFRQGYLIPWKKCRPEAWKSAATLLKSDRGGLVYQPLVGVYED
                     VIELDFVSMYPTIMVRCNVSPETINCPCCKPQPDVPELGYTICRRRRGLVSAALEPII
                     ARRALFKSEMKRLRAAADPAAADLFRQRQEALKWLLVCCFGYLGYRNARFGRIEAHET
                     TTAFSRDKLLRAREICEDRGFRVLHAIVDCVWIQREGATDAEIADLCREINAATDLTI
                     ALEGRYRWLAFLPSRMNPDMPVPNRYFGLFEDGTLKYRGIEVRRSDQAPYVQRVQREL
                     LDALAAAPSLAACRALRGRLYRIVQEAERRLRERAVDLEDLIFKRKTSMEADDYRGNG
                     MTALAARQMKQAGLTLHAGEVVRYLVLSEGDRDLNARIRVPSLLGPDDAFDVEFYVNQ
                     LRRAAATLLEPILAEPLPPL"
     gene            4051..4710
                     /locus_tag="PLS90_14900"
     CDS             4051..4710
                     /locus_tag="PLS90_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03734.1"
                     /translation="MPHRGFTLIELLVVVAVIAILAAIAVPNFLDAQIRAKVARTKAD
                     LRTLATAVEAYAVDHQGYPVRRNSEGDGRVQPHVPELTLRLQQMSVITTPVGYISSLP
                     QDVFENHLPYPENVLDYWDSTQHSWLINSRHILRPERHINPGTAGYLLVSVGPDTYLG
                     PTASRYGWPSIARMEGTVFFPYDPTNGTVSDGNIYVSQSMGAEETGAEMTARLDPRRP
                     R"
     gene            4869..>5055
                     /locus_tag="PLS90_14905"
     CDS             4869..>5055
                     /locus_tag="PLS90_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03735.1"
                     /translation="MPGCPLLWLALLALAACRPAPPGGDAPQPAGDDSAPADAPAAYI
                     LDSQPFSPTLPHLFEEGR"
BASE COUNT          748 a         2231 c         1244 g          832 t
ORIGIN      
        1 cgtctggctg cccgattcga tggaaggccc cacgcccatc agcgcgctga tcgcctgtgg
       61 cgaatccccg ccctcgaccg acgcgggccg cgcttccgac gacgcccccg ccaccccgac
      121 cgtcaccctc gccctcaact ggttccctga ggccgaacat ggcggctact acgccatgaa
      181 ggccgatcgc ctcgacgccg ccgagggact caacgtcgtg atcaagccgg gcggccccgg
      241 caattccccc atcccgctcg tcgtcaccgg ccaggccgat tacgccgtct gcaatgccga
      301 cgacgtcctc ctcgcccgcg cccagggcgc cgacgtcgtc gcgctgatgg ccccgctcca
      361 ggtcagcccg cgctgcatca tggtccacgc cgactccgcc atcgagcgcc tcgaagacct
      421 gcgcgacgtc acgctcgcca tgaacgccgg caaacccttt gccgcctggc tgcaggcccg
      481 cgtcccgctc gacggcgtcc aggtcgtccc ctattccggc ggcgtcgcgc cattcctcct
      541 cgacccgcgc tatgcccagc aggcctacgt cttcagcgag ccctacctcg cccagcagca
      601 gggcgcctcc gtccgcagcc tgatgctccc cgacatcggc ttcaacccct acaccagcgt
      661 cctgatcacc accgccgcgc gcctggccgc cgacgaagcc caggccgccg ccatggttcg
      721 cgccgccgtc gccggctggc gccgctacct cgaacaaccc gccccggcca acgccctgat
      781 tcacgccgcc aaccccgaaa tgagcctcga gattctccaa tacggcgccg acgtcctgcg
      841 cgacctggtc cagcccgcgt cgctccccgc cgagcaaatc ggaaccatga ccccgcaacg
      901 ctgggccgaa ctgaccggcc aactggagga actccaactc ctcccggccg gcgccgtcaa
      961 cgccgaggac gcctacaccc tccgcttcct cccccaaccc taaacctcgt ccctcgtccc
     1021 tcgtcctccc cctcgccccc agaggccccg ccccaagatt cgtccctcgt cctcgtccct
     1081 catcctcggc tctcgaaaac ccgcctctcc cccctcatca acccatcaac tccgtccgat
     1141 attccatccc aacgcgacca aactcaaacc agccttcgtg gagtgcggtg gcatagcgtt
     1201 gctacgcaac gcgcgacacc gctttggatc gacccaccct cgccgatttc gatcatcctc
     1261 ctcacttgat tgcgcctgga ccgcccggtg tcgattcctt tcctggttgc cccgtccatg
     1321 tccccgcccg tgttctcgag ttgcctcttt tgaattttga ggcttcgaat ttggttcgaa
     1381 tttcgccatt cgaatttcgt gcttcttctc aagccgtcct ccttccccgc cgccctattt
     1441 tcgctttttc ttcgtatttt ccttgcccct gctccccctc agctcctaca ctcaaactca
     1501 gaggcctcgc gccccccttc tgccccctgg attctccgtc tacttaacgt ctctcccgtt
     1561 cctcccgtcc cttatgtccc tgcctccccc acccttcccc cagaaaaatc aggatcacca
     1621 cgccgacggc tggctgctgg acctctaccc ccaggccgcc gacatgatcc tctggttcat
     1681 cctcgacgac ggccgccgcc tgcgcctgcg cgatcccttc acccccatct tctatgtcag
     1741 ctttgaaggc cagcccgacc cggagcaatc gcgccgggcc ctgcgcgccg ccctggcccg
     1801 gctcgacggt ctggagccgg ccgggcccgc ccgccgcctc gatttctggt ccggcgacca
     1861 gatcgacgtc gaggccatcc gcgtcacgaa cctcgacctg gccggaccca acctccgcgc
     1921 cctcgcccgc caactccccg aactgacctg ctacaactgc gatatcgccc ccgaaatcca
     1981 ttacgcctac gaccgcggcc tgttccccac cgcgcgctgc cgcctcacct ggcgcggcga
     2041 cacgctcacc gcctgcgaac tgctcgaccg cgccgatgac acgcgctacg acctcccccc
     2101 cctgcgcgaa gcccaactca ccgcgcgcgg caacctcctc ggccgccgcc cccgcctgct
     2161 cagcctctcg ctcgcctgcg agggccgcac cctgacctgg gacgacgccc ctccggccga
     2221 aatgctccgc tcgctgcgcg acgccctccg cgcgtgcgac cccgacctgc tctggaccga
     2281 agggggcgac gccgccctgc tgcccgccct gctcacgctc gcccagcgcc atcgcgctca
     2341 actcgggccc gacgccgacc tcgccctcga ccgcgagccc ggcatccgcc gccgcctccg
     2401 caccgacggc cacaccttcg tctcctacgg ccaggtcctc taccaggccc ccgattaccc
     2461 cctcttcggc cgctggcaca tcgatcgcca caactccttc tgggccggca cgatcggcct
     2521 ggccggcctg gtcgaggtcg cccgcctcgc ccgcttgccc gtccagcgcg ccgcccgccg
     2581 ctcgatcggc accggcatct cctccatcca gctccatcac gcctttcgcc agggctacct
     2641 catcccctgg aaaaaatgcc gccccgaagc ctggaaaagc gccgccacgc tcctcaagtc
     2701 cgaccgcggc ggcctggtct accaacccct cgtcggcgtc tatgaggacg tgatcgaact
     2761 ggatttcgtc tcgatgtacc ccaccatcat ggtccgctgc aacgtctccc ccgaaacgat
     2821 caactgccct tgctgcaaac cacagcccga cgtccccgaa ctcggctaca ccatctgccg
     2881 ccgccgccgc ggcctcgtct ccgccgccct ggagccgatc atcgcccgcc gcgccctctt
     2941 caaaagcgaa atgaagcgcc tgcgcgccgc cgccgacccc gccgccgccg acctcttccg
     3001 ccagcgccag gaggccctca agtggctcct cgtctgctgt ttcggctacc tcggctatcg
     3061 caacgcccgc ttcggccgga tcgaggccca cgaaacgacc accgccttct cccgcgataa
     3121 actcctgcgc gcccgcgaga tctgcgagga tcgcggcttc cgtgtcctcc atgccattgt
     3181 cgattgcgtc tggatccagc gcgaaggggc caccgacgcc gaaatcgccg acctctgccg
     3241 cgaaatcaac gccgccaccg acctcaccat cgccctcgag ggacgctacc gctggctggc
     3301 cttcctcccc tcgcgcatga accccgacat gcccgtcccc aaccgctact tcggcctgtt
     3361 cgaggacggc acgctcaaat accgcggcat cgaggtccgc cgcagcgatc aggcccccta
     3421 cgtccagcgg gtccagcgcg aactgctcga cgccctggcc gccgccccct cgctggccgc
     3481 ctgccgcgcc ctgcgcggcc gcctctatcg catcgtgcag gaggccgagc gccgcctgcg
     3541 cgaacgcgcc gtcgatctgg aggacctcat cttcaaacgc aaaacctcca tggaagccga
     3601 cgattaccgc ggcaacggca tgaccgccct cgccgcgcgc cagatgaaac aggccggcct
     3661 gacgctccac gccggcgaag tcgtccgcta tctcgtcctc agcgagggcg accgggacct
     3721 caacgcgcgc atccgcgtcc ccagcctgct cggccccgac gacgccttcg acgtcgagtt
     3781 ctacgtcaat cagctccgcc gcgccgccgc caccctcctc gaaccgatcc tcgccgaacc
     3841 cctccccccc ctctaactct cctctctcgc ctctcgtccc tcgtccgcgg ctcccgaatt
     3901 tcctgccccc ccagccaaaa attgacgccc accacccctt tcgcctaaac tgcttgggcg
     3961 ggtccggggg aacccgccag ccgaccctcc accctttttc gatcagctcc ggtcccctgc
     4021 cgccccggag cgggaagtaa gccgtcgccc atgccccatc gtggtttcac gctcatcgag
     4081 ctgctggtgg tcgtggcggt catcgccatt ctggccgcca tcgccgtccc caacttcctc
     4141 gatgcccaga tccgcgccaa ggtcgcccgc accaaggccg acctgcgcac cctcgccacc
     4201 gccgtcgaag cctacgccgt cgatcaccag ggctaccccg tccgccgcaa ttccgagggc
     4261 gacggccgcg tccagcccca tgtccccgag ctcaccctcc gcctccagca gatgtcggtc
     4321 atcacgaccc ccgtcggcta tatctccagc ctcccccagg atgtcttcga aaatcatctc
     4381 ccctaccccg aaaacgtcct cgattactgg gattccaccc agcattcctg gctcatcaac
     4441 tcgcgccata tcctccgccc cgagcgccac atcaaccccg gcaccgccgg ctatctcctc
     4501 gtctccgtcg gccccgacac ctacctcgga cccaccgcca gccgctacgg ctggccctcc
     4561 atcgcccgca tggaggggac cgtcttcttc ccctacgacc cgaccaacgg gacggtctcc
     4621 gacggcaaca tctacgtcag ccagtcgatg ggcgcggagg aaaccggagc ggaaatgacc
     4681 gcgcgcctgg atccgcgtcg gccccggtga ccccctcctc ccgccggctt tcccgtggac
     4741 acgccgggcg cccccgcgtt acacaggaag cctgtccccc cgcgcggagc gccccccggc
     4801 cgcgcgcgac ccccttcgtt ccggtgcgcc gcctgattcc catgaaccct ctccttcatc
     4861 gttttgcggt gcccgggtgc cccttgctct ggctggcgct gctggccctc gccgcatgcc
     4921 ggcccgcgcc ccccggcggc gacgcccccc aaccggccgg cgacgactcc gcccccgccg
     4981 acgcccccgc cgcctacatc ctcgacagcc aacccttctc ccccaccctc ccccatctgt
     5041 tcgaggaagg ccgca
//
LOCUS       DAOPCO010000290         5054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13990_length_5054_cov_5.399080, whole genome shotgun sequence.
ACCESSION   DAOPCO010000290 DAOPCO010000000
VERSION     DAOPCO010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13990_length_5054_cov_5.399080"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLS90_14910"
     CDS             <1..347
                     /locus_tag="PLS90_14910"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPK03736.1"
                     /translation="AEDVLMAETNPSFVPRSETLEKSSDLGSRLGMVLEPAEDGDGLR
                     VRDVRPGSPAQVAGIKNGDIIRKVNGQTVRSADELKKIIEAAKEGAPLAVMLEQEGRM
                     TLTVIQLDKASD"
     gene            467..1204
                     /locus_tag="PLS90_14915"
     CDS             467..1204
                     /locus_tag="PLS90_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03737.1"
                     /translation="MGIAGRSASMPKRLSFRARLANFPTRGLQRYLPANPGPRKWTNM
                     ITNSRPWFPMVASAFALGFASTLAAQPAAADVAPAATPWWTPLISTGGIVTLVLLVVV
                     AAAIILFMAGRRPRVDVDGYFSENEMEGSKYEQLLAEIQGLSLRVKNGEGKGDFRKIE
                     KLVRVFLERSGFIGVRKMTDEQLMALLAGGQVPQAQASSLASIIERCKRGAQSEHEKL
                     DFSALELLRELRELVWANETPPAHKSA"
     gene            1264..2286
                     /locus_tag="PLS90_14920"
     CDS             1264..2286
                     /locus_tag="PLS90_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPK03738.1"
                     /translation="MALHEAGTPTVRSQDDLLHLQETIRRESEFLPALVAELRKHIVG
                     QQAMIERLLIGLLCGGHVLLEGVPGLAKTLTIRTLADAINLSFHRLQFTPDLLPADII
                     GTLIYNQKDGNFTPKKGPIFANLILADEINRAPAKVQSALLESMQERQVTIGEATYPL
                     PSPFMVMATQNPIEQEGTYPLPEAQVDRFMLKVLIHYPSREEERRILDLMTEGEPPRV
                     EPVVQPETLLRARRAVEAIYVDEKIKNYIVSLVMATRDPEAAGVPISAYLQFGASPRA
                     TIFLARAARAHAFLCRRGHVIPDDVKMIGPDVLRHRLIVSYEAEAEGRTTDDLIRTLF
                     DHVEVP"
     gene            2514..2726
                     /locus_tag="PLS90_14925"
     CDS             2514..2726
                     /locus_tag="PLS90_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPK03739.1"
                     /translation="MAKPNTALITKGEKYYVAQCVEPGVVSQGETIDEAKSNLQEAVE
                     LYLEGMAGERLPAFSSEVIVYPLEVA"
     gene            2854..2940
                     /locus_tag="PLS90_14930"
     CDS             2854..2940
                     /locus_tag="PLS90_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03740.1"
                     /translation="MHKELDRNTRMGILRQCGLTKSDLEHLL"
     gene            2986..3882
                     /locus_tag="PLS90_14935"
     CDS             2986..3882
                     /locus_tag="PLS90_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPK03741.1"
                     /translation="MPDLDLATLQQKVRRIEIVSNRLVSERVAGEYHSVFKGQGIEFE
                     EVRPYTYGDEIRSIDWNVTARAGEPHIKRYREEREMTVFFVVDLSGSCRFGTRVEFKS
                     ELMAEVCAVLSLAAIANNDQVGLLLCTDRLEKVVPPRKGRRHVLRLIREVLAFEPQGD
                     GTDLTRALDHLRHVLHRRAVVFVISDFLTRGFEQSLAVVRQRHDVIAIAVEDPIEHEL
                     PDAGLIELIDSETGRLTIVDSGSRRVREDYRRQVEQRRLARRRLCRKLEVDLVELRTD
                     ASYELELIKFFRLRVRRLRHRV"
     gene            3894..>5054
                     /locus_tag="PLS90_14940"
     CDS             3894..>5054
                     /locus_tag="PLS90_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPK03742.1"
                     /translation="MTPVGPYQHTSYRGSRRRPWLAILLLLVVAGGLVAVLTDYSPAR
                     LFGASRAAIEKDFARAARLSSQGHHVLAAGIYERVSADDDVAPDQRTQAEVMLARIYR
                     EHLNNPGAAALAEERARRLAQQAGNINLPGEESATRKLSMAEWARRAEVDLRQPEGEV
                     NVIATLGDQTVSLEEVLFAWQFFNGNLPPRGEDFRDFVNSYLDLVLLADEAVRRELNL
                     RNRFYYDMRIQRLTTLNRLLQFELGREIAAPSPEELADFADRLSLALSEPASVTVGLI
                     VVDSERAADRLADALEDDDADFARLAADYSLAHNDLEQGYIAGSITEHDQELPRFGRN
                     PRLVQRLTAMEAGATTGPLRSAQGWFFFKVLARNAGRPARVDVSEPGLLERYQ"
BASE COUNT          910 a         1784 c         1508 g          852 t
ORIGIN      
        1 ccgcggagga cgtgctgatg gccgaaacca atccttcctt tgttccgcgg tccgagacct
       61 tggaaaaatc ctccgacctc ggctcgcgtc tcggcatggt cctcgaaccg gccgaggatg
      121 gcgacggact gcgcgtccgc gacgttcgcc ccggctcgcc cgcgcaagtc gccggcatca
      181 agaacggcga catcatccgc aaggtcaacg gccagaccgt gcgctccgcc gatgaactga
      241 aaaagatcat cgaagccgcc aaagaggggg cgccgctggc ggtcatgctg gaacaggagg
      301 ggcgcatgac gctgaccgtg atccagttgg acaaggcgtc cgactaggcc ctgccggctc
      361 cagcgccgcg agatccatcg tgcaacgggg accggctcgg cggcccgacg gcccgactca
      421 agaaaatggc tgttgcctcg ccgcgccgcc ccccgttgct ccccgcgtgg ggattgcggg
      481 ccgctccgcg agcatgccga aacgcttgtc tttccgcgcg cggctcgcta atttcccaac
      541 acggggcctt cagcggtatc tgcctgcaaa tccggggcct cgcaagtgga cgaacatgat
      601 caccaactca cgaccctggt ttccgatggt tgcgtcggcc ttcgccctag gcttcgcgtc
      661 gacgctggcc gcccagcccg ccgcggcgga cgtcgccccc gcggccactc cctggtggac
      721 gccgctgatt tcgaccggag gcatcgtcac cctggtgctg ctggtcgtcg tcgcggcggc
      781 gatcatcctg ttcatggccg gccggcgtcc gcgcgtcgat gtcgacggct acttctccga
      841 aaacgaaatg gagggctcga aatacgagca gttgctcgcg gaaattcagg gcctttcgtt
      901 gcgggtcaag aacggcgagg gcaagggcga ctttcgcaag atcgaaaaac tcgtgcgcgt
      961 ctttctcgaa cgctccggct tcatcggcgt ccgcaagatg accgacgagc agttgatggc
     1021 cctgctggcg ggcggccagg tcccgcaggc ccaggcctcc tcgctggctt cgatcatcga
     1081 gcgctgcaag cgcggcgcgc aaagcgagca cgagaaactc gattttagcg ccctggaatt
     1141 gttgcgcgaa ctgcgcgaac tggtctgggc gaacgaaacc ccgcccgccc acaaatccgc
     1201 ctgagcgggc ggtcgttcgc gtcccgcgcc atgcaccagg gcgatcaaca cgaggagaac
     1261 gccatggctt tgcatgaagc gggaaccccg accgtccggt ctcaggacga tctgctccat
     1321 ctccaggaaa ccatccggcg cgaaagcgaa ttcctgcccg ccctcgtcgc cgaactgcgc
     1381 aagcatatcg tgggccagca ggcgatgatc gaacgcctcc tgatcggcct gctctgcggc
     1441 gggcacgtgc tgctcgaggg cgtccccggt ctcgccaaga cgctcaccat ccgcaccctc
     1501 gccgacgcga tcaacctctc cttccaccgc ctgcagttca cccccgacct gttgccggcc
     1561 gacatcatcg gcacgctgat ctataaccag aaggatggca acttcactcc caagaagggg
     1621 ccgatcttcg ccaacctgat cctcgccgac gagatcaatc gcgccccggc caaggtgcaa
     1681 tcggccctcc tcgaatcgat gcaggagcgc caggtcacca tcggcgaggc gacctatccc
     1741 ctcccctcgc cgttcatggt catggccacg cagaacccga tcgagcagga agggacctat
     1801 ccgcttcccg aggcgcaggt cgaccgtttc atgctcaagg tcctgatcca ctatccgtcg
     1861 cgcgaggagg aacgccgcat cctcgatctg atgaccgagg gggaaccgcc gcgcgtcgag
     1921 ccggtcgtgc agcccgagac gctgctgcgc gcgcgccgcg ccgtcgaagc gatctatgtg
     1981 gacgagaaga tcaagaacta catcgtcagc ctggtgatgg cgacccgcga cccggaggcc
     2041 gccggcgtcc cgatcagcgc ctacctgcaa ttcggcgcct cgccgcgcgc cacgatcttc
     2101 ctggcccgcg ccgcccgcgc ccacgccttt ctctgccgcc gcggccatgt catccccgac
     2161 gacgtcaaga tgatcggccc cgacgtgctg cgccaccgcc tgatcgtcag ctacgaggcc
     2221 gaggccgagg gccgaacgac cgacgacctg atccgtacgc tgttcgatca cgtcgaagtg
     2281 ccgtgagcaa cgctcgtcgg cttgggcatc gggcgcgacc tgagctggga gtcgaagtat
     2341 cggcagggga ttgcgccgaa tcagcgtcga ataccgccaa ggacacccgg ttctggcaaa
     2401 tcggcaggcc cgatcggtca acaatgacaa gcccagccag cgacggcatt attggatcac
     2461 gtagacaagt gctcatctcc acagcgcatt catttttttg gagggaccag aaaatggcta
     2521 aacccaatac cgcattgatc accaaaggtg aaaagtacta tgtggcacaa tgcgtggaac
     2581 cgggcgttgt ctctcaggga gagacgatcg atgaggccaa atcaaattta caggaagccg
     2641 tcgaattgta cttggagggc atggcggggg aaaggcttcc agctttctca agtgaagtca
     2701 ttgtctatcc ccttgaggtc gcctgatggc tgacctgccc gactgatcgg gaagagcgat
     2761 gatcaagcta cttgagcgcc gcggttttgt tgtctgccgc cagaaggcag ccatgtcttt
     2821 ctgaaacgag gggaaactgg caccgttgtc ccgctgcaca aggagttgga tcggaatact
     2881 cgaatgggga ttttgaggca gtgcggatta acaaagtcag acttggagca tcttctttga
     2941 actatcgcca gttgcagttc ttcgaatcaa agatttctag aacccatgcc cgaccttgat
     3001 cttgccacgc tccagcagaa agtccgccgg attgagatcg tctcgaaccg gctggtcagc
     3061 gagcgcgtcg ccggcgaata ccacagcgtc ttcaagggcc agggcatcga attcgaggag
     3121 gtccgcccct acacctacgg cgacgagatc cgctcgatcg actggaacgt caccgcccgc
     3181 gcgggcgaac cgcacatcaa gcgctaccgc gaggagcgcg agatgaccgt cttcttcgtc
     3241 gtcgatctct ccggctcgtg ccgcttcggc acccgcgtgg agttcaagag cgagctgatg
     3301 gccgaggtct gcgccgtcct ctcgctcgcc gcgatcgcca acaacgatca ggtcggcctg
     3361 ctgctttgca ccgaccgcct ggaaaaagtc gtcccgccgc gcaagggccg ccgccacgtc
     3421 ctgcgcctga tccgcgaagt gctcgccttc gaaccccagg gcgacggcac cgacctgacc
     3481 cgcgccctcg atcatctgcg tcacgtgctc catcgccgcg cggtcgtctt cgtcatctcc
     3541 gacttcctca cccgcggctt cgaacagtcg ctcgccgtcg tgcgccagcg ccacgatgtc
     3601 atcgccatcg ccgtggagga ccccatcgag cacgagctgc ccgacgccgg cctgatcgag
     3661 ctgatcgaca gcgaaacggg tcggctgacc atcgtcgatt cgggcagccg ccgcgtccgc
     3721 gaggattacc ggcgccaggt cgagcagcgg cgcctcgcgc ggcgccggct ctgccgcaaa
     3781 ttggaagtcg atctcgttga actgcgcacc gatgcctcct acgaactgga gctgatcaaa
     3841 ttcttccgcc tgcgcgtgcg gcgcctccgc caccgggtct agaaagtccc ctcatgacgc
     3901 ccgtcggccc atatcagcac acctcctacc gcggctcgcg ccgccgcccc tggctggcca
     3961 tcctcctgct gctggtggta gccggcgggc tggtggcggt cttgacggat tattcgcccg
     4021 cccggctgtt cggtgcgtcg cgcgccgcca tcgagaagga cttcgcccgc gccgcccgcc
     4081 tttccagcca gggccatcac gtcctggccg ccggcatcta tgaacgcgtc agcgccgacg
     4141 acgatgtcgc ccccgatcag cgcacccagg ccgaggtgat gctcgcgcgc atctaccgcg
     4201 agcacctgaa caatcccggc gcggccgccc tggccgagga acgcgcccgc cgcctcgcgc
     4261 agcaggcggg caacatcaac ctgccgggcg aggaatccgc gacgcgcaaa ctctccatgg
     4321 ccgaatgggc ccgccgcgcc gaggtcgatc tgcgccagcc cgagggcgag gtcaacgtca
     4381 tcgccaccct gggcgaccag accgtttcgc tcgaggaggt cctgttcgcc tggcaattct
     4441 tcaacggcaa tctgcccccg cgcggcgagg acttccgcga cttcgtcaac agctacctcg
     4501 atctggtcct gctcgccgat gaagccgtcc gccgcgagct caacctccgc aaccgcttct
     4561 actacgacat gcgcatccaa cgcctcacga cgctcaaccg gctcctccag tttgagctgg
     4621 gccgcgagat cgcggccccg tcgcccgagg aactcgccga cttcgccgac cgcctctccc
     4681 ttgcgctctc cgaacccgcc agcgtcaccg tggggctgat cgtcgtcgac agcgaacgcg
     4741 ccgccgaccg cctggccgac gccctggagg atgatgacgc ggatttcgcg cggctcgccg
     4801 ccgattacag cctggcccac aacgatctcg agcaaggcta catcgccggc tccatcaccg
     4861 agcacgacca ggaactgccc cgcttcggcc gcaatccccg cctggtccag cgcctgaccg
     4921 ccatggaggc cggcgccacg accgggccgc tgcggtccgc ccagggctgg ttcttcttca
     4981 aggtcctcgc gcgcaacgcc ggccggcccg cccgcgtcga tgtctccgag ccggggctgc
     5041 ttgaacgcta tcag
//
LOCUS       DAOPCO010000291         5052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_13999_length_5052_cov_5.518511, whole genome shotgun sequence.
ACCESSION   DAOPCO010000291 DAOPCO010000000
VERSION     DAOPCO010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13999_length_5052_cov_5.518511"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..2198
                     /locus_tag="PLS90_14945"
     CDS             453..2198
                     /locus_tag="PLS90_14945"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HPK03743.1"
                     /translation="MIAALFVASCIVLALAYRYYGAFLNRQFQIDDSRPTPSTTMTDG
                     VDYVPASRPVLFGHHFSSIAGAGPVVGPIIAATWFGWGPVMAWILVGSILIGGVHDFG
                     SLVMSIRHRARSIAEICRLYLSPLTYRFFLMFIWLALVYVLVVFIDLTAASYMAAPAS
                     DPDQGGTVATASMLYIVLAIVFGLLTRRGLKLGLGSLIFVPLVFAALLVAHWLPTDPQ
                     HIRLLAPAGSYFYEHPKYIWSAILLAYCFIASVTPVWLLLQPRDYLSSYLLFACLGLG
                     GLGLLISGALGRVPIQYEFFAGLQNAELGFIFPALFITVACGAVSGFHSIVASGTTAK
                     QLPQESAVRPIAYGTMLTEGALALVALATVMVLADRPAGNPMLTFADGIGQFLSVLGL
                     PADLGTVFGLLAVSTFLLTTLDTCTRLARYVVEEFFGIGNPRWRYATTLASVLPLVYF
                     AFKEYPNPASPGAMIPAWRVIWPAFGTTNQLLAALALLVVLVWRRAQGRSYWFILWPM
                     LFMLASTGASMVLMIVENLSPGAEGAVPVIGWINLLMLLMTLWLVGDTVLNWGRLGRV
                     AAAESEGLTASATAG"
     gene            2195..3253
                     /locus_tag="PLS90_14950"
     CDS             2195..3253
                     /locus_tag="PLS90_14950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK03744.1"
                     /translation="MNPGPPPLRVLVVTNMYPTPAAPYYGVFVKEQVDALRELGVEVG
                     LEIHLGRESKWNYACGLASLTRRLRRERWDLIHSHHTFTTLTALAARRLAGRGRGPRR
                     IPIIESFHEGEVFDPRGRWRGHLRHSAWLKALALRRADFVLPVEKNMIRAALAAAGAA
                     DAEVEAERIPHQVLPAGIDLERFGPGDAQGARRRLGWDGEGPLLFFPYAPGKPAKRDD
                     LARAAFAIVRRTHPGAQLVVGGAIPYAAMPDAIRASDVLLILTDYEASPTIVKEALAC
                     ERPVVATDVGDIRACYGDLPGVVVTPAEPEAIASGIDRALALPAGSACGGRERLIARG
                     LTLPATARRLVEVYRELI"
     gene            complement(3289..3729)
                     /locus_tag="PLS90_14955"
     CDS             complement(3289..3729)
                     /locus_tag="PLS90_14955"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPK03745.1"
                     /translation="MKLSCCHYGPMDHQAGARSRLARAIVVLALCPLLLLYGTACSLF
                     AGSRQNISVNSDPAGAKVVIAGMPVGNTPLQYQIRKNEEAMITVSKEGYDSATGVTST
                     SLSTLGILDIIGGCIFLLPFFGLLAPGAYEQEPNNFYFSLNQND"
     gene            complement(3881..4918)
                     /gene="thiE"
                     /locus_tag="PLS90_14960"
     CDS             complement(3881..4918)
                     /gene="thiE"
                     /locus_tag="PLS90_14960"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPK03746.1"
                     /translation="MAAHRRMLDANLNRLEEGLRVVEDAARFELGDAAAAARLKRLRH
                     DAGDLRQRFGGLTAARDSLGDPGRRRIAEPARRPTFASVVGASFGRVQQSLRVIEELA
                     KLEDAAAARRAKAMRYAAYDLEKQLMPRLARHAAAARLRGLYLILSRPVAGYEKLAEL
                     AVRRKVGAIQLRAKELESAELLALARRLRAITSGSSTLFFVNDRADIARLSEADGLHL
                     GQRDLSVADARRIVGDRMLVGKSTHNRRGLTAALREAPDYVGIGPLFTTQSKEQPDPT
                     LGIDGAARLLRGAGVPAVAIGGITAANLADVLAAGFRSYALIAAVCQAREPERVLREL
                     RRRERQAGITA"
BASE COUNT          730 a         1673 c         1768 g          881 t
ORIGIN      
        1 gcaagttttg ggcgtggggc cgcggggcag ggggtggccg cgggcatcac tcccgtgccg
       61 gggcgaatag gggcgcgagc cgccacggcc cgggcagcca gcggcgcagc agagcggtgg
      121 agagggacgg gctggcgtca tggcaagaca ctcccggtca gccctttgga ctcggtatag
      181 aaatttgagc tcacagtccg gcatgctcga aaacgccacc gggccggaag agccgaattt
      241 tgggtaggcc gttgggggca aggccaatca ggggagtggc gcttccaggt ttcagccggt
      301 cttcgagagc cgaggacgag ggacgatgtt gaggggaacg aggggggcgc ggtgtgcgcc
      361 aaccgaattg actctccgcc ccgtattcac gaacatggga cgcaggggat tcggggcaat
      421 ctgtccatct tgcccattga ggatgacccg ggatgatcgc ggcactcttc gtcgccagtt
      481 gcatcgtgtt ggcgctggcc tatcgttatt acggggcgtt tctgaaccgc cagtttcaga
      541 tcgacgactc acgccctacg ccgtcgacga cgatgaccga cggggtcgat tacgttccgg
      601 cgtcgcggcc ggtgctgttc ggccaccatt tcagttcgat cgccggggcg gggccggtcg
      661 tcgggccgat catcgccgcc acctggtttg gctgggggcc ggtgatggcg tggattctgg
      721 tcggctcgat cctgatcggc ggcgtccacg atttcggctc gctggtgatg agcatccgcc
      781 accgcgcccg gtcgatcgcc gagatctgcc ggctctacct cagcccgctc acttaccggt
      841 tcttcctgat gttcatctgg ctggcactgg tttacgtgct cgtcgtgttc atcgacctga
      901 ccgcggccag ctacatggcc gcgccggcgt cggaccccga ccagggggga accgtcgcga
      961 ccgcatcgat gctctatatc gtgctggcaa tcgtgttcgg cctgctgacg cggcgcgggc
     1021 tgaagctcgg cctcggttcg ctgatcttcg ttccactcgt cttcgcggcg ctgctggtcg
     1081 cgcactggct gccgaccgat ccgcagcaca tccggctgct ggcgcccgcc ggctcgtatt
     1141 tttacgaaca cccgaagtac atctggtcgg cgatcctgct ggcgtattgc ttcatcgcgt
     1201 cggtcacgcc ggtgtggctg ctgctgcagc cgcgcgacta cctgagctcc tatctgctgt
     1261 ttgcctgcct gggcctgggc gggttggggt tgctgatcag cggcgcgctg gggcgcgtgc
     1321 cgattcagta tgagtttttc gccggcttgc agaacgccga gctgggcttc atcttcccgg
     1381 ccctgttcat cacggtggcg tgcggcgccg tcagcgggtt ccattcgatc gtcgcctcgg
     1441 gcacgaccgc caagcagctt ccccaggagt cggccgttcg cccgatcgcc tacggcacga
     1501 tgctgaccga aggggcgctg gcgctggtcg cgctggcgac ggtgatggtg ctcgccgacc
     1561 ggccggcggg caatccgatg ctgacgttcg ccgacggcat cggccagttc ctgtcggtgt
     1621 tgggcctgcc ggccgatctg ggcaccgtgt tcgggctgct ggccgtgtcg acgtttctgc
     1681 tgacgacgct ggatacctgc acgcgcctgg cgcggtatgt cgtcgaggag tttttcggca
     1741 tcggcaatcc ccgctggcgc tacgccacga cgctggccag cgtgctgccg ctggtgtact
     1801 tcgcgttcaa ggaatacccg aacccggcca gtccgggcgc gatgatcccc gcctggcgcg
     1861 tgatctggcc ggcgttcggc acaaccaacc agttgctggc ggcactggca ctgctggtgg
     1921 tgctcgtctg gcggcgggcg cagggacgct cctactggtt cattctctgg ccgatgctgt
     1981 tcatgctggc cagcacgggg gcgagcatgg tgctgatgat cgtggagaac ctgagccccg
     2041 gcgccgaggg ggcggtgccg gtcatcggct ggatcaacct gctgatgctg ctgatgacgc
     2101 tgtggctggt gggcgacacg gtgctgaact ggggccggct ggggcgcgtc gcggccgcgg
     2161 agagcgaagg cctgaccgcg tcggcgacgg cgggatgaac cccgggccgc cgccgttgcg
     2221 cgtgctggtc gtcacgaaca tgtatcccac gccggcggcg ccgtattacg gcgtgtttgt
     2281 gaaggagcag gtcgacgcgc tgcgcgagct gggggtcgag gtcggcctgg aaatccatct
     2341 cggccgcgaa tcgaaatgga attatgcatg cgggctggcg tcgctgacgc gccggctgcg
     2401 ccgcgagcgg tgggacctga tccacagcca tcacaccttt acgacgctga cggcgctggc
     2461 ggcgcggcgg ctggccgggc gcggccgggg tccgcggcgg atcccgatca tcgagagctt
     2521 tcatgagggg gaggttttcg atccgcgcgg ccgctggcgc ggccatctgc gccattcggc
     2581 gtggctgaag gccctggcgc tgcgccgggc ggatttcgtg ctgccggtcg agaagaacat
     2641 gatccgcgcc gcgctggcgg cggccggcgc cgccgacgcc gaggtcgaag ccgaacggat
     2701 cccccaccag gttctgccgg cggggatcga tctggagcgc ttcgggcccg gcgacgcgca
     2761 aggcgcccgc cgccgcctgg gctgggacgg cgaggggccg ttgctttttt ttccctacgc
     2821 gccgggcaag ccggccaagc gcgacgatct ggcccgcgcc gccttcgcga tcgtgcggcg
     2881 gacgcatccc ggcgcgcaac tggttgtcgg cggcgcgatc ccttacgcgg cgatgcccga
     2941 tgcgatccgg gccagcgatg tgctgctgat cctgaccgat tacgaggcgt cgccgacgat
     3001 cgtcaaggaa gcactggcct gcgaacgccc ggtggtcgcc accgacgtgg gcgacattcg
     3061 ggcgtgctac ggcgatctgc cgggggtggt cgtgaccccc gccgagccgg aggccattgc
     3121 gtcggggatc gatcgggcgc tggccctgcc ggccggttcc gcgtgcggcg ggcgtgaacg
     3181 gctgatcgcc cggggcctga cgctgccggc caccgcccgg cggctggtcg aggtgtatcg
     3241 cgagctcatt tagtcgcgat tctgccgcgc gcccccgcag agaccggctc agtcgttctg
     3301 attcagcgag aaatagaagt tgtttggctc ctgctcatat gctcccggcg cgagcaggcc
     3361 gaagaacggc agcaggaaga tgcaaccgcc gatgatatcg aggatgccga gcgtgctcag
     3421 gctggtcgag gtcacgccgg tggcgctgtc gtaaccctcc ttgctcacgg tgatcatcgc
     3481 ctcttcgttc ttccggatct ggtactgcaa cggcgtgttg ccgaccggca tgccggcgat
     3541 gacgactttg gcgccggccg gatcggaatt gaccgaaatg ttctgacgcg acccggcgaa
     3601 gagcgaacag gcggtgccat acagcagcag cagcgggcag agcgccagga ccacgatggc
     3661 gcgcgcgagc cgggagcgcg cccccgcttg atggtccatc ggcccgtaat gacaacagga
     3721 cagtttcata tcatcccctc caacggcaca tgccccggga gaacctgatg cgggtttcgc
     3781 gggaagtcca tgacggtatc ggacagccgg acgaagcgca gtctagacgg gtcgcgcggc
     3841 gatatcaagc gccgcgccgg gcgggccgac ggatcgcggg tcacgccgtg attccggcct
     3901 ggcgttcgcg ccggcgcagc tcgcgcagca cgcgttccgg ttcgcgcgcc tggcagaccg
     3961 ccgcgatcag cgcataggat cgaaagcccg ccgccagcac atcggccaga ttcgcggcgg
     4021 tgatgccgcc gatggccacg gccggcacgc cggcgccgcg caacagccgc gccgcgccgt
     4081 cgatacccag cgtcggatcg ggctgttcct tcgattgcgt ggtgaacagc ggcccgatcc
     4141 ccacataatc gggagcttcc ctgagcgccg ccgtcaatcc gcgccgattg tgcgttgact
     4201 tgcctacgag catccgatcg ccgacgatcc gccgcgcatc ggccaccgac aggtcgcgct
     4261 gacccaggtg aagcccgtcg gcctcgctca gccgcgcgat gtcggcccgg tcgttcacga
     4321 agaacagcgt cgagctgccg ctcgtgatcg ctcgcagccg gcgcgccagc gcgagcagct
     4381 cggcgctctc cagttccttc gcccggagct ggatcgcgcc gactttgcgc cgcaccgcca
     4441 gttccgccag tttttcgtaa cccgccaccg gccggctgag gatcaagtag agcccgcgca
     4501 gccgcgccgc cgcggcgtgc cgggccaggc gcggcatgag ctgcttttcc aggtcgtagg
     4561 ccgcgtagcg catcgccttg gcgcgccgag ccgcggccgc gtcctcgagt ttcgccagtt
     4621 cctcgatcac ccgcagcgac tgctgcaccc ggccaaacga agcgcccacg accgatgcga
     4681 acgtcggccg ccgcgccggc tcggcaatcc ggcggcggcc ggggtcgccc aggctgtcgc
     4741 gcgcggcggt cagaccgccg aaccgctgcc gcaaatcgcc ggcgtcatgg cgcagtcgct
     4801 tgagtcgcgc cgccgccgcc gcatcgccca actcgaaccg tgccgcgtcc tccaccacgc
     4861 gcagcccttc ctccaggcgg ttcagattgg cgtcgagcat gcggcggtgc gcggccatgg
     4921 cggcgggaca acccccttcg cggagatcgt ccgattatgc cgggcctgaa ccggagagac
     4981 cagcgattac gcgatgcgtg tcaaagcaca atagaaatgt cacccctttg agcacaatag
     5041 aagtgtcacc tg
//
LOCUS       DAOPCO010000292         5047 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14027_length_5047_cov_4.630409, whole genome shotgun sequence.
ACCESSION   DAOPCO010000292 DAOPCO010000000
VERSION     DAOPCO010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14027_length_5047_cov_4.630409"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLS90_14965"
     CDS             <1..609
                     /locus_tag="PLS90_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPK03747.1"
                     /translation="ADTEDVWHALFAEMGRQYREPTLVLFSDGVESACGFADAAVGPF
                     YCPADQQVYLDLTFFDELNRRFGAPGDFAQAYVIAHEIGHHVQHQLGAMDRVQSARGR
                     QTQAAANEKSVRLELQADFLAGVWAHHAQRTRAILEPGDIEEALGAASAVGDDTLQRR
                     AQGRVVPDAFTHGTAEQRMRWFRKGFETGDVAQGDTFAVPEP"
     gene            complement(1234..2325)
                     /locus_tag="PLS90_14970"
     CDS             complement(1234..2325)
                     /locus_tag="PLS90_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HPK03748.1"
                     /translation="MEGHAPRPAPGSPAALGYRHPAEWERHAATWLAWPHNRRDWPGK
                     FAPVQWVYAEIVRRLAPGERVRILVQDARHEALAASQLRRAGARLERVEFFQIETDRG
                     WCRDMGPIFVVREQPAPRAAIADLHFNAWAKYANWRKDARVAGELARRLGMPRLDVRT
                     ADGRPFVLEGGAIDVNGAGVVLTTEECLLDPTTQVRNPGLTRDEVERALGDALEARQV
                     VWLGRGIAGDDTHGHVDDLCRFVNPTTIVLCQEPDGADANHRPLAENRERLEGLRLAD
                     GEKAEVIALPMPEPLWFDGLRLPASYANFYIGNEVVLVPTFNDPRDREALGILGECFP
                     TRTVVGIHAVDLVWGLGTLHCLTHEEPAV"
     gene            complement(2325..3221)
                     /locus_tag="PLS90_14975"
     CDS             complement(2325..3221)
                     /locus_tag="PLS90_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPK03749.1"
                     /translation="MPQPRKFTVGLLQMALQRDPEANLTHAAGMIRRAAADGAEVICL
                     PELFRSPYFCQREDAALFDLAEPIPGPATEALAGIARECQAVILAPLFERRAAGVYHN
                     SVAVLDADGRLAGIYRKMHIPDDPCFYEKFYFTPGDLGFRAFDTRYGRIAPLICWDQW
                     YPEAARLAALRGACLLLYPTAIGWHPSEKATHGEAQREGWITIQRSHAIANGLYVAAV
                     NRIGLEKDERGGDGLEFWGSSFVCDPFGTILAQAPVDQEAIVTAEVDPARIEETRRGW
                     PFLRDRRIDAYGDLTRRLIDEG"
     gene            complement(3327..3403)
                     /locus_tag="PLS90_14980"
     tRNA            complement(3327..3403)
                     /locus_tag="PLS90_14980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3367..3369),aa:His,seq:gtg)
     gene            3791..>5047
                     /locus_tag="PLS90_14985"
     CDS             3791..>5047
                     /locus_tag="PLS90_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03750.1"
                     /translation="MRRSHLIRRPPSRTWLATLLLLALAAVAARAADRFDVIGGDGIV
                     PINPNFNPDTSLIVTLPNGDQIDLASTQITGQGFLGRINFQQTAFALGQNLSQDLSAA
                     LTQSLSASNRPSVVTPYSGILVFDPATGQPIYDSFGSLVTNKANTIGKGKIGLGLSYQ
                     HATFDSFDGEDIGRQVDTTIVSEQDIEIPSTFIFESITGDVLTRSTEPFRSVIDIRAR
                     NVEFDADVVTLTLIYGLLENVDIGALIPYIWLTTRGEVEIKVDQSVVLESEVNLEGSD
                     TPLIVPLGAASSTRKFKGEFDRDFDGIGDIILFTKWQILSQFGLPKRTKAPLDLALQF
                     EVKLPTGDEDEFLGTGHTDLALRMLMQRELTKRFILRGELGGSISTLGSEYSSFDYKL
                     GIEGLITNDLAGGVELIGSYTRAFESV"
BASE COUNT          832 a         1878 c         1526 g          811 t
ORIGIN      
        1 gccgataccg aggatgtctg gcatgccctg ttcgccgaga tgggacggca ataccgcgag
       61 ccgaccctcg tcctcttctc cgacggcgtc gagtcggcct gcggcttcgc cgacgccgcc
      121 gtcggcccgt tctactgccc cgccgatcag caggtctacc tcgacctgac cttctttgac
      181 gagttgaacc gccgcttcgg cgcgccgggc gatttcgccc aggcctacgt gattgcccac
      241 gagatcggcc accacgtcca gcaccagctc ggcgccatgg accgcgtgca gtcggcccgc
      301 ggccgccaga cccaggccgc ggcgaacgaa aaatcggtcc gcctcgaatt gcaggccgac
      361 ttcctggccg gcgtctgggc gcaccatgcc cagcgcaccc gcgccatcct cgagcccggc
      421 gacatcgagg aagcgctcgg cgccgccagc gccgtcggcg acgacaccct ccagcgccgc
      481 gcccagggcc gcgtcgtccc cgacgccttc acccacggca ccgccgagca gcgcatgcgc
      541 tggttccgca agggcttcga aaccggcgac gtcgcccagg gcgacacctt cgccgtgccc
      601 gagccgtaga tgcccggatg gtcttcggtc tttcccttca cctgactcac catgccaacc
      661 ttcatcagaa cgtctttctc gcggccggaa acggaccggc aaccggccga ccccgcggat
      721 cggtggcgct tgcggttcat gattgctgtt gctgtgtgcc cggctctcga aacttctgtt
      781 tgaaatgctt gtctagtcga ttcctaagat cggcgccttt tgcaacctga tcggacccac
      841 ccagcgccaa taggcggtca ccatgctggt gttgacggtg cggaggacgt tgccgcgcgc
      901 tgttcgagga tcgagacgat gcggtcgaag agggcgtcgg gtcgcgtcgt ggcgagtttg
      961 cccatcgctt tactcatcgt cagctccccg gggaaccggg ctgggtgcgg cgagtgggtt
     1021 cggcgaaggg cgagttgaag atcgggtttt cgatgacgac ggaatccacc gcaacctccc
     1081 caagttcccc ctggcctacg gtgaaagaac aaagcagttg agacggaacc ggttcaaggt
     1141 aaagaaacgg cgcggcggga tcaaggggga aaacagggta gacgcggtga gtctggagac
     1201 tgtttgccgt ggcgaagccg cacggcaacc ccgtcagacc gccggttcct catgcgtcag
     1261 gcagtgcagc gtccccagcc cccacaccag gtcgacggca tggatcccca cgaccgtgcg
     1321 cgtcgggaag cactcgccca gaatccccag cgcctcgcgg tcgcgcggat cattgaacgt
     1381 cggcacgagc accacctcgt tgccgatgta gaagttggca tagctcgccg gcagccgcag
     1441 cccgtcaaac cacagcggct cgggcatcgg cagcgcgatc acctcggcct tctccccgtc
     1501 cgccagccgc agcccctcca gccgctcgcg gttctccgcc agcggccggt gattggcgtc
     1561 tgcgccgtcc ggctcctggc acaagacgat cgttgtcgga ttgacgaacc ggcacaggtc
     1621 gtcgacgtgg ccgtgcgtgt cgtcccccgc gatcccccgg cccagccaga ccacctgccg
     1681 cgcctccagc gcatcgccca gcgcccgttc cacttcgtcg cgcgtcagcc ccggattgcg
     1741 gacctgcgtc gtcggatcca gcaggcattc ctcggtcgtc agcacgaccc ccgcgccgtt
     1801 gacgtcgatc gcgcccccct ccagcacgaa gggccttccg tcggccgtcc gcacgtcgag
     1861 ccgcggcatc cccagccggc gcgccagctc ccccgccacc cgggcgtctt tccgccagtt
     1921 ggcgtatttg gcccaggcgt tgaagtgcag gtcggcgatc gccgcccgcg gcgccggctg
     1981 ctcccgcacc acgaagatcg gccccatgtc gcggcaccac ccgcgatccg tctcgatctg
     2041 gaaaaactcc acgcgctcca gccgcgcccc cgcccgccgc aactggctgg cggccagggc
     2101 ctcgtgccgg gcgtcctgga ccaggatccg cacccgttcg cccggcgcca gccgccgcac
     2161 gatttcggca tacacccact gcaccggcgc gaacttcccc ggccagtcgc gccggttgtg
     2221 cggccacgcc agccatgtcg ccgcgtgccg ctcccattcg gcggggtggc ggtagcccaa
     2281 ggccgccggc gatcccggcg ccggccgcgg cgcgtgtccc tccatcagcc ttcgtcgatc
     2341 agccggcgcg tcaggtcgcc gtaggcatcg attcgccggt cgcgcaggaa tggccagccg
     2401 cgccgcgtct cctcgatccg cgccggatcc acctcggccg tcacgatcgc ttcctggtcc
     2461 accggggcct gcgccaggat cgtcccgaac ggatcgcaca caaaactcga tccccagaac
     2521 tccagcccat cgccgccccg ctcgtccttt tccagcccga tccggttgac ggcggccacg
     2581 taaagcccgt tggcgatggc atggctgcgc tggattgtga tccacccctc gcgctgggcc
     2641 tcgccgtggg tcgccttctc gctcggatgc cagccgatgg cggtcggata gagcagcagg
     2701 catgccccac gtagcgccgc cagccgcgcc gcctcggggt accactgatc ccagcagatc
     2761 agcggcgcga tccggccgta gcgcgtgtcg aacgcccgga accccaggtc gcccggcgtg
     2821 aaatagaatt tctcgtagaa gcacgggtcg tcggggatgt gcatcttgcg gtaaatcccc
     2881 gccagccggc cgtcggcgtc cagcaccgcc acgctattat ggtagacgcc cgcggcgcgg
     2941 cgttcgaaca gcggcgcaag gatgacggct tgacactcgc gtgcgatccc cgccagcgcc
     3001 tcggtcgccg gccccgggat cggctcggcc aggtcgaaca gcgccgcatc ctcccgctgg
     3061 cagaagtagg gcgaacggaa cagttcgggc aggcagatca cttcggcccc gtcggccgcc
     3121 gcccgccgga tcatcccggc cgcatgcgtc agattggcct ccgggtcgcg ctgaagcgcc
     3181 atctgcagca ggccgacggt gaatttccgg ggttggggca tgacgtcagc gggcgggggc
     3241 ggatcgggcc gcaacggatc ggggccgggt agagcagcgg gccgaccggg ccgaagccgg
     3301 cccgatcggc cccctcgcgc gaaacctggg gtgggtaatg ggactcgaac ccacggcctt
     3361 tggagccaca atccaacgct ctaaccaact gagctatacc caccgcataa gcgccggccc
     3421 gccccccgca acccgggcgg cggcgccgac aaacacccta cgttagggaa atcgagcgcg
     3481 ccgcgtcaag gggtttcacc cgccccgccc cggccgccct gccgccgcgt aactccccgc
     3541 gccgcaatcc ttttcgcccc cgatgaaaaa cttgacattt tttcgggcag agggaagcat
     3601 ggcgggtaag ttgtgccgat aggatacggg tacgttctgg ttcggatgta ggagcggagc
     3661 gacgcgtgcg tatccggcga tcccgtcacc gccgaaggca catcctccag ttgagcgtgc
     3721 ggcggcgcct cgcgcgttgc cccatgtcaa tcccgttgat cgcgcccccg cgcataaggt
     3781 tggtgctgcc atgcgtcgat cgcacctgat ccgccgcccc ccttcccgga cgtggctggc
     3841 cacgctgctg ctcctggcgc tcgccgcggt cgccgcgcgc gccgccgatc gcttcgacgt
     3901 catcggcggc gacggcatcg tcccgatcaa ccccaacttt aaccccgaca ccagcctgat
     3961 cgtcaccctg cccaatggcg accagatcga tctggcgagc acccagatca ctgggcaggg
     4021 cttcctcgga cgcatcaact tccagcagac cgccttcgcc ctcggccaga acctctcgca
     4081 ggatctgagc gccgccctca ctcagtcgct cagcgccagc aaccgcccca gcgtcgtcac
     4141 cccctactcc ggcatcttgg tgttcgatcc ggccaccggc cagccgatct acgactcgtt
     4201 cggctcgctg gtgaccaaca aggccaatac gatcggcaag ggcaagatcg gcctcggcct
     4261 gagttaccag cacgccacgt tcgacagctt cgacggtgag gatatcggcc gccaggtcga
     4321 taccaccatc gtctcggagc aggacatcga gatcccttcg accttcatct tcgaatcgat
     4381 caccggcgac gtcctcaccc gctccaccga accctttcgc tcggtcatcg acatccgcgc
     4441 ccgcaacgtc gagttcgacg ccgacgtcgt caccctgacc ctgatctacg gcctgctcga
     4501 aaacgtcgat atcggcgccc tgatccccta catctggctg acgacgcgcg gcgaagtcga
     4561 aatcaaagtg gaccagagcg tcgtgctgga gtcggaggtt aatctcgagg gaagcgacac
     4621 cccgctcatc gtcccgctcg gcgccgccag cagcacccgc aaattcaagg gcgaattcga
     4681 ccgcgacttc gacggcatcg gcgacatcat cctgttcacc aagtggcaga tcctctcgca
     4741 gttcggcctg cccaaacgca ccaaagcccc cctcgacctc gccctgcagt tcgaagtcaa
     4801 gctccccacc ggcgatgagg acgagttcct cggcaccggc cacaccgatc tcgccctgcg
     4861 catgctgatg cagcgcgagc tgaccaagcg cttcattctg cgcggcgagc tgggcggcag
     4921 catctccacg ctcggcagcg aatacagttc cttcgattac aaactcggca tcgagggcct
     4981 gatcacgaac gacctcgccg gcggcgtcga gctgatcggc agttatacgc gggccttcga
     5041 aagtgtg
//
LOCUS       DAOPCO010000293         5039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14051_length_5039_cov_5.905498, whole genome shotgun sequence.
ACCESSION   DAOPCO010000293 DAOPCO010000000
VERSION     DAOPCO010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14051_length_5039_cov_5.905498"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLS90_14990"
     CDS             complement(<1..826)
                     /locus_tag="PLS90_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPK03751.1"
                     /translation="MSPSPELPPPSDDPPPPAGIGADAAAPADAGPDPADPPVIPGKG
                     ITPEEIDRIPALPGVYIMRDAAGKAIYIGKAANLRARVGSYFKRSGDARFNVQFLMRH
                     VQRVETIITANEKEAFLLENTLIKKHQPRYNLRLRDDKTYVSVRINLDHEWPRAVVMR
                     RRAEARRDKAVYLGPYASAHGVRETLRQLQRVFPIRSCTDHVLRNRTRPCLLHAIGRC
                     CAPCVKPVDKDEYRELVEGTILFRKGKTREVVRMLQERMARHAEAMEFEQAAVVRDR"
     gene            complement(854..2131)
                     /locus_tag="PLS90_14995"
     CDS             complement(854..2131)
                     /locus_tag="PLS90_14995"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HPK03752.1"
                     /translation="MTDQLAAAPLQPETPGRPFSGFLPRTGAFLADCFVLGAAAYLIS
                     ESLRPALSLAKPWLPLLGQVLVFLYFWLMDGVSGQGQTLGKKLLGVRVVDDAGRQPAS
                     AAAARRALLKLPVFLPLVEIRLLALNLPLGARPAFFIAFQIVNIAAMTYFITLAAGLA
                     MHPRKRAWHDLWAGTYVTRDPVPAGFHEALAAEVDPLASARMRNHLKMTLIFWLVATA
                     ALLIRPVRHLSDPELHADLERVEALERERPLDPYVVLAAQYPSDHARRQFEEQVRLYH
                     QQSAAAGRQALTTDTLRRRVDDGQTIHIAAVLPRGTFDPAQLDDPAFRRQVERLRSLV
                     WEHWRSRAAATSQPPPPALAFDLVLADPFQLLFEPLVMLVKNESEARARVTGPPDPEL
                     GPLVYELILAPPPAPAAQDPLTTSPATQPPGAP"
     gene            complement(2128..3258)
                     /gene="hisC"
                     /locus_tag="PLS90_15000"
     CDS             complement(2128..3258)
                     /gene="hisC"
                     /locus_tag="PLS90_15000"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPK03753.1"
                     /translation="MSARSATPPPLNRHLRRLLPYPPGKPIEEVKREFGLSEVCKLAS
                     NENPLGPSPRALKAMAAVAGEMNLYPDGSAYYLKQALAERLGVSPAELILGNGSDEII
                     VFLALAYLNRRRDLVTSDYAFVRYRMAAELAGAPCRLAPMRDMRHDLRALARHITPQT
                     AMVCLDLPGNPTGASVSRRELERFLRAIPPSVIVVLDQAYFEYAVADPDYPDGLALRA
                     RFPNLVVTRTFSKAYGLAGLRIGYAVARPEIVTDLERIRPPFNTTRVSQAAAIAALDD
                     HAHLRRSTAANAQGLAQLRQGFDRLGLKQWPSLANFILVDVGRDGREVFQALLRAGIV
                     TRPMAGIGLPTGLRISVGTPRQNRACLAALGRILREGAQAAS"
     gene            3423..4190
                     /locus_tag="PLS90_15005"
     CDS             3423..4190
                     /locus_tag="PLS90_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014161087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK03754.1"
                     /translation="MQPPANARERFSNRVEHYLATRPKYPDALYDVLARETGLDADWT
                     IADIGSGTGFLSEVFLRAGNRVFCIEPNEPMRAGGERYLRGKGEFHSVAGSAEATTLG
                     DRSVDLVAAGQAFHWFDAAAARAEWRRILREPGWVALVWNTRRNDGSPFMREYEALLA
                     GLGDYANVRHRHFGDERMEALFGARGWVRHAMPNHQRLDRAGLRGRLLSSSYSPAPGS
                     EAAAAALAKVDALFERHAADGMVELLYETELYVGRVR"
     gene            complement(4457..>5039)
                     /gene="lepB"
                     /locus_tag="PLS90_15010"
     CDS             complement(4457..>5039)
                     /gene="lepB"
                     /locus_tag="PLS90_15010"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPK03755.1"
                     /translation="RPMTVAPTHAPLADSPPRLRRRLRVMVGLLILLVLMGVNLAIAS
                     GKVRFEKVISGSMEPTLLIGDVLLMDANAVPDQYDIVVLNDPEEPGGKLVKRIIGMPG
                     DRIVLRGGILYLNGKEEYSTQVTSNHISWEDTRFKVPEDHVFVLGDNRNNSVDSLNFG
                     PVSYEEIRGVVSAVIWPPSRWRKPAPLHPEPAE"
BASE COUNT          763 a         1508 c         2005 g          763 t
ORIGIN      
        1 gccggtcgcg gacgacggcc gcctgctcga actccatcgc ctcggcgtgg cgcgccatgc
       61 gctcttgcag catccgcacc acctcgcgcg tcttcccctt gcggaagagg atcgtgccct
      121 ccaccagttc gcggtattcg tccttgtcga ccggtttgac gcagggggcg cagcagcggc
      181 cgatcgcatg cagcaggcag gggcgcgtcc ggttgcgcag aacgtggtcg gtgcaggagc
      241 ggatggggaa gacgcgctgg agttggcgca gggtctcgcg gacgccatgg gccgaggcat
      301 aggggccgag gtagacggcc ttgtcgcggc gggcctcggc gcggcggcgc atgacaaccg
      361 cgcgcggcca ctcgtggtcg aggttgatcc gcacgctgac gtaggtcttg tcgtcgcgca
      421 gacgcaggtt gtagcgcggc tgatgtttct tgatcagcgt gttttcgagg aggaaggctt
      481 ccttctcgtt ggcggtgatg atcgtctcga cgcgctggac gtggcgcatc aggaactgga
      541 cgttgaagcg ggcgtcgccc gagcgtttaa agtaggagcc gacgcgcgcg cgcaggttgg
      601 ccgccttgcc gatgtagatc gccttgccag cggcgtcgcg catgatatag acgcccggca
      661 gcgcggggat gcggtcgatc tcctcgggcg tgatcccctt gccggggatg accggcgggt
      721 cggcggggtc cggcccggcg tcggcggggg cggcggcgtc cgcgccgatc ccggccgggg
      781 ggggcgggtc gtcactgggc ggcggaagtt ccggggaagg cgacatggga ttctggccgc
      841 gcgcgccggc ggttcatggg gcgccgggcg gctgcgtcgc gggggaagtg gtcaacggat
      901 cctgggcggc gggcgccggg ggcggggcga ggatcaactc gtaaaccagc ggccctagtt
      961 cggggtcggg cgggccggtt acgcgcgccc tagcctccga ttcattcttg accagcatca
     1021 ccagcggttc aaacaaaagc tggaacgggt cggccaggac cagatcaaag gcgagggccg
     1081 gcggcggggg ctggctcgtc gcggccgcgc ggctccgcca gtgctcccag accaggctgc
     1141 gcaggcgttc cacctgccgg cggaaggcgg ggtcgtcgag ctgcgcgggg tcgaacgttc
     1201 cgcggggcag gacggcggcg atgtggatcg tctggccgtc atcgacgcgc cgacgcaggg
     1261 tgtcggtcgt caacgcctgc cggccggcgg cggccgattg ctggtgatat aggcgcacct
     1321 gctcctcgaa ttgacggcgg gcgtggtcgc tcggatactg cgcggccagc acgacgtacg
     1381 ggtccagggg gcgctcgcgc tcgagggctt cgacccgttc caggtcggcg tgcagttccg
     1441 ggtcgctcag gtggcgcacc gggcggatca gcagcgcggc ggtcgcgacc agccagaaaa
     1501 tgagggtcat cttcaggtgg ttgcgcatgc gggcggaggc cagcgggtcg acctcggcgg
     1561 cgagcgcctc gtgaaagccg gcgggaacgg gatcgcgcgt gacgtaggtg ccggcccaca
     1621 ggtcgtgcca ggcgcgcttg cggggatgca tggcgaggcc ggcggcgagc gtgatgaagt
     1681 aggtcatcgc cgcgatgttg acgatctgga aggcgatgaa gaacgcgggc cgcgctccga
     1741 gcggcaggtt gagcgcgagc aggcggatct cgacgagggg caggaacacc ggcagcttga
     1801 gcagggcgcg gcgggcggcc gccgcgctgg cgggttgacg cccggcgtca tcgacgacgc
     1861 gcacgccgag cagcttcttg cccagcgtct gcccctggcc cgagacgccg tccatcagcc
     1921 agaagtagag gaaaacgagg acctgaccga gcagcgggag ccacggcttg gcgagcgaga
     1981 gcgcggggcg cagcgactcg ctgatgaggt aggcggccgc acccagcacg aagcaatcgg
     2041 ccaggaacgc gcccgttcgg ggcaggaaac cggagaacgg ccggcctgga gtctcgggtt
     2101 ggaggggcgc cgcggccagt tggtcggtca tgaggcggcc tgcgcgcctt cgcggaggat
     2161 ccggccgagc gcggccaggc aggcccgatt ctgccggggc gtgccgacgg agatgcgcag
     2221 gccggtgggc aggccgatgc ccgccatcgg ccgcgtgacg attccggcgc gcaggagggc
     2281 ctggaagact tcgcgcccat cgcgcccgac atcgaccagg atgaagttgg ccaggctggg
     2341 ccattgcttc aggccgagcc ggtcgaagcc ctggcggagc tgggcgaggc cctgggcatt
     2401 ggcggcggtg cttcggcgca ggtgcgcgtg gtcgtccagc gcggcgatcg ccgccgcctg
     2461 ggaaacccgc gtcgtgttga acggcggccg gatccgttcc aggtcggtga cgatttcggg
     2521 gcgggcgacg gcgtagccga tgcgcagacc ggccaggccg taggccttgg agaaggtccg
     2581 cgtgacgacc agattgggga agcgggcgcg cagcgccaga ccgtcggggt aatccggatc
     2641 ggcgacggca tactcgaagt aggcctgatc cagaacgacg atgacggagg gggggatggc
     2701 gcgcaggaag cgctccagct cgcgccgcga aaccgaggcg ccggtgggat tgccgggcag
     2761 atcgaggcag accatggcgg tctggggcgt gatgtgccgg gcgagcgccc gcaagtcgtg
     2821 gcgcatgtcg cgcatcggcg ccagccggca gggggcgccg gccagttcgg cggccatccg
     2881 gtaacggacg aaggcatagt cggaggtaac caggtcgcgg cgccggttca ggtaggccag
     2941 cgcgaggaaa acaatgattt cgtcggaacc gttgcccagg atgagttcgg cgggggagac
     3001 gccgagtcgc tcggcgagcg cctgcttgag gtaataggcc gagccgtcgg ggtagagatt
     3061 catttccccg gcaacggcgg ccatcgcctt gagcgcgcgc ggcgaggggc cgagcgggtt
     3121 ctcgttggaa gcgagcttgc agacttcgct caggccgaac tcgcgtttga cttcctcgat
     3181 cggcttgccc ggcgggtagg gcagcaggcg gcgcagatgg cgattgagcg gcggcggcgt
     3241 ggcggagcgg gcggacatgg ggtcggggtc atcctttgcg gacaagatgc aaccggcgat
     3301 tattggcggg cggggaagca gtgtcaaacg ccgcgcgagg ggcgcctgga agcgaccggc
     3361 gcttccgctt gcgggccggg gttgctacgc tacgttttga gttgaacgga gtcagatcaa
     3421 agatgcagcc gcccgccaat gccagggaac gcttttccaa tcgcgtcgag cactatctgg
     3481 cgacgcggcc gaagtatccc gacgccctgt acgatgtgct ggcgcgcgag accgggctcg
     3541 acgccgactg gacgatcgcc gacatcggct cggggacggg cttcctgagc gaggtcttcc
     3601 tgcgcgcggg caaccgggtg ttctgcatcg agccgaacga gccgatgcgc gcggggggcg
     3661 aacgttacct gcgcggcaag ggggagttcc acagcgtggc cgggtcggcc gaagcgacga
     3721 cgctcggcga ccggtccgtc gatctggtcg cggcggggca ggcgtttcac tggttcgacg
     3781 ccgcggccgc gcgcgccgag tggcggcgca tcctgcggga gccgggctgg gtggcgctgg
     3841 tctggaacac gcggcggaac gacgggtcgc cgttcatgcg cgagtatgaa gcgctgctgg
     3901 ccgggctggg cgattacgcg aacgtgcgtc accgccattt cggcgacgaa cggatggagg
     3961 cgctcttcgg cgcgcggggc tgggtgcgcc atgcgatgcc taaccatcag cggctcgatc
     4021 gtgcggggtt gcgcggccgg ctgctctcca gttcctacag cccggcgccg gggagcgagg
     4081 ccgcggcggc ggcgctggcg aaagtggatg cgctgttcga gcgacacgcc gcggacggga
     4141 tggtggagct gctctatgag acggagctct acgtgggccg ggtgcgctga gggcgcatgg
     4201 ctgtgatcgg cgcgcttgaa aatccggcca ccccttccag gggtttgagg cgtcgggcgg
     4261 cgacgcaggg ttccggcttg cggcctcccc cctgcgcttt attccagccg tccgctccgc
     4321 ggactcatgg tgtctgacgg gcggctggga tgaatgaagg cgccgccgga actgtcggct
     4381 attgggtggc gccagatctg aatgcgtgcg gtcggagaaa gaccggcgag ggcgccggtc
     4441 ccacgccggc cggcatctac tcggcgggtt cggggtggag gggggcgggt ttgcgccagc
     4501 ggctgggcgg ccagataacg gccgaaacga cgccgcggat ttcctcgtag ctcaccgggc
     4561 cgaaattcag actgtccacg ctgttgttgc ggttatcgcc caggacgaag acgtggtcct
     4621 cggggacctt gaaccgggta tcctcccagg agatatggtt ggaggtgacc tgggtggaat
     4681 actcctcctt gccgttcaga tagaggatgc cgccgcgcag gacaatgcgg tcgccgggca
     4741 tgccgatgat gcgcttgacc agtttgccgc ccggctcctc cggatcgttg aggacgacga
     4801 tgtcgtactg gtcggggacg gcgttggcgt ccatcagcag gacgtcgccg atgaggagcg
     4861 tcggctccat cgagccggag atgacctttt cgaagcggac cttgcccgag gcgatcgcca
     4921 gattgacgcc catcaggacc agcaggatca gcaggccgac catgacgcgg aggcggcggc
     4981 gcagccgcgg gggggagtcg gcgagggggg cgtgggtcgg ggcaacggtc atcggtcgg
//
LOCUS       DAOPCO010000294         5008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14150_length_5008_cov_5.341611, whole genome shotgun sequence.
ACCESSION   DAOPCO010000294 DAOPCO010000000
VERSION     DAOPCO010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_5008_cov_5.341611"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLS90_15015"
     CDS             <1..628
                     /locus_tag="PLS90_15015"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK03756.1"
                     /translation="QSPGVEREIEAVAAHRRLVGVRHQVESSPDDAWLAREASIRGLR
                     ALAAAGLPFDLIVFPRHLDTIPILAERAPELRMVLDHLGKPDIAHGGFAAWAPRLRRI
                     AQFPNIYCKISGLVTEADHHRWRLDDLKPYVQFARECFGIDRLMWGSDWPVCLKAADY
                     RATLDSALQTIGPLSPEEREQFLAGNARRFYRLDQPAFATTDSEEQGG"
     gene            945..1649
                     /locus_tag="PLS90_15020"
     CDS             945..1649
                     /locus_tag="PLS90_15020"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPK03757.1"
                     /translation="MGKLINNGRKKVRYEALTQQVYESIKGEILDMRLKPGDRLTIDA
                     LARDMGVSISPVRETLARLLAERLVEFKPYSGYVVAQLPSKKYFIDMVSTRILLECHA
                     VRIGAPRRSETIIRELEEALAGMDSHSLGTRYRDFRGYLEWDIRFHGLLIRSAENEVL
                     IQIYEDLRSLNQFSRLYLYIGKQINQSRVRTEHEAILKAYREGDGEKAARAVRSHLES
                     TGKNIEKIGEPSAMMV"
     gene            1801..3903
                     /locus_tag="PLS90_15025"
     CDS             1801..3903
                     /locus_tag="PLS90_15025"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPK03758.1"
                     /translation="MKLENALYRLDLDPKRGTIRQIRDKRADLDLIADPRIAEPFRLL
                     LPLPEQEANYIYGRDQAAPAAEKTRTAAHFRWEGPLTSELGRVYSMGVSVHYELIEEA
                     IHCRIDVENRSDLTLAEVWYPMVGGLTGYGERRETRSFIPCAGSTYEAQIFQNFPESM
                     GIGGGSGNTYPEFHLSYPGQMPMAWVDFYNPRRKRGLYFALHETIARFGVMRLEMHPG
                     VAHNTAGDNWPRPEEIEPGAPVGVLMNWVKFPYVEPDGRFTGHPMILQTHEGDWHQAA
                     RIYSAWFRSQFSVVDPRASWLHREQVYQDVISLLPEGNVHLRFRDIPQFAQDGLDYGV
                     KALIVSSYNVGGHDAKIPSYDDPDPRLGTWEELKAAIAACHERGVKVFFFVNLQPVDM
                     ALDWYEQELKNYVVQDRWGIHAMHMGWGMGTLGARLGYTRRPLVPCNPGIARFREIIV
                     RQLVHLAEIGADGVHFDKLGWPIGLDFNPLSDFTPDRGPAEGILLALRETLDACRRIN
                     PHFVISCEYWWDRLLEFATVAWAWHTQLDHEPALKFTFPEWMPMMPVVQPYAWNVVNN
                     ATRYGYQVMVGAARFSRSMQDPPMRPLSQYIAEVLRIREELVDFIQFGEFLDTQDARV
                     EAHEDVRFNVHRHRESGKRACVIVNLGLKDRPVKLRLKGNHKGRVELHAPFKKKKTAN
                     LPLQVKLPPRGLLIAVEQ"
     gene            4023..>5008
                     /locus_tag="PLS90_15030"
     CDS             4023..>5008
                     /locus_tag="PLS90_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03759.1"
                     /translation="MSRLRPFAALLLLPILRPAAVSTAAPEPMGYLHEGSPVTELPIQ
                     LSRGKYRKTDLGYRADEASTITSLEVRFADLTGGDSLASKETGIQFFAALDAGNARPL
                     VPAIPLAVALSPGQLLPLTLWIRPGDQIDEGPFTAQLRMGTSHGEESLPLSLSIHPGL
                     TPDAGWPEWRYDERRMARSPLKGRIDQPVTKWEIPLGASSQPEVLNQTLGYSIPPVPA
                     WVAAGATARVADLDPDNPGLEVAVFHGISRLGELFTYQGELLWSVTAPREMYTPILQF
                     ADVDQDGLLEIIVTSHGGVSVWDGATGHLDYDIPYQVGRQYGHSVAVNIDADP"
BASE COUNT          929 a         1705 c         1490 g          884 t
ORIGIN      
        1 ccaatccccc ggcgtggaac gggagatcga ggccgtcgcg gcgcaccggc ggctggtcgg
       61 cgtgcgccat caggtcgagt ccagccccga cgacgcatgg ctggcgcgcg aggcttcgat
      121 ccgcgggctg cgcgcgcttg ccgccgccgg cctccccttc gatctgatcg tctttccgcg
      181 ccatctcgac acgatcccca tcctggccga acgggcgccc gaactgcgca tggtgctcga
      241 tcatctgggc aagcccgaca tcgcccatgg ggggtttgct gcctgggcgc cccgcctgcg
      301 ccggatcgcg cagttcccca acatctattg caagatctcg ggcctcgtca ccgaggctga
      361 tcaccaccgc tggcgactgg acgatttgaa gccctatgtg cagttcgccc gcgagtgctt
      421 cggcatcgac cggctgatgt ggggcagcga ctggccggtc tgcctgaaag cggccgacta
      481 tcgcgcgacg ctcgacagcg cgctccagac gattggcccg ctcagtccgg aagagcgcga
      541 gcagttcctc gccggcaacg cccgccggtt ctaccggctc gaccagccgg ctttcgccac
      601 gaccgattcc gaggaacagg ggggttaacg gaacggccgc tgtgtctctt acgtccatta
      661 cgtcctttgt gtcccttcgc tcatgaaccg gcggtttaca acgagggaca gaagacggtg
      721 ccgtgaatgg ccgattcatg aggccagggc cttgccggac cgatcagcgg gccagtcccg
      781 tcagagacgt tagaactgcc caaatcaatc acgatcacga ggacgtggga cgaagacgag
      841 gggcgacatc aacgtgagac gccttgacgc cgtgacgccc ttttgctgaa atttagccgg
      901 aaaaatcgtt ggcccgcgcc gcgggcaaag ggatggctcc tccattggga aaactcatca
      961 ataacggccg gaaaaaagtc cggtatgaag ccctgacgca acaggtttat gaatcgatca
     1021 agggcgaaat cctcgacatg cgcctcaagc cgggcgaccg tctgacgatc gacgccctgg
     1081 cccgcgacat gggcgtcagc atctcacccg tccgcgaaac gctcgcccgg ctcctcgccg
     1141 aacggctcgt tgagttcaaa ccgtattccg gctacgtcgt ggcccagctc ccctcgaaga
     1201 agtacttcat cgacatggtc agcacccgca tcctgctcga gtgccacgcc gtgcggatcg
     1261 gggcgccgcg gcgctccgag acgatcatcc gggagctgga ggaagcgctc gccgggatgg
     1321 actcccattc gctcgggacc cgctatcgcg atttccgggg ctatctggag tgggatattc
     1381 gcttccatgg cctgttgatc cgcagcgccg agaacgaagt gctgatccag atctacgaag
     1441 acctgcgctc gctgaaccag ttcagccggc tttacctcta catcggcaag cagatcaacc
     1501 agtcgcgcgt gcgcaccgag cacgaggcca tcctcaaggc ctaccgtgaa ggggacggcg
     1561 agaaggccgc ccgggcggtc cggtcgcacc tggaatcgac cggcaaaaac atcgagaaga
     1621 tcggcgaacc ctcggcgatg atggtctgag agggggccgt cgcgcgtgct ggtggacggc
     1681 gcggcgccgg cggcgttttg ctttgacatg aaataatgca tgatcgatca tcggagatcc
     1741 cgcatccgcc ggcgcgcgct aacccatctc cggttgcaac ccatcgcccg aaggaccgtc
     1801 atgaaactcg aaaatgccct ctaccgcctc gacctcgacc ccaagcgcgg cacaatccgc
     1861 cagatccgcg acaagcgcgc cgatctcgat ctcatcgccg acccccgcat cgccgaaccc
     1921 ttccgcctcc tgcttcccct ccctgaacag gaggccaact acatctacgg acgggaccag
     1981 gccgcgcccg cggccgagaa gacccgaacc gccgcccatt tccgctggga ggggccgctc
     2041 accagcgaat tgggccgcgt ctactcgatg ggcgtgtcgg tccattatga actgatcgag
     2101 gaggcgatcc attgccggat cgacgtggaa aaccggtcgg atctgacgct cgccgaggtc
     2161 tggtatccga tggtcggcgg cctcaccggt tacggcgagc gccgggagac gcgcagcttc
     2221 atcccatgcg cgggatcgac ctacgaagcc cagatcttcc agaatttccc cgaatcgatg
     2281 ggcatcggcg gcggcagcgg caacacctac cccgagtttc atctaagtta ccccggccag
     2341 atgccgatgg cctgggtcga tttctacaat ccgcgccgca agcgcggcct ctatttcgcc
     2401 ctgcacgaga cgatcgcgcg cttcggcgtc atgcgcctcg aaatgcaccc cggcgtcgcc
     2461 cacaacacgg ccggcgacaa ctggccccgc cccgaggaga tcgaacccgg cgcccccgtc
     2521 ggcgtcctga tgaactgggt caagtttccc tacgtcgaac cggacgggcg cttcaccggc
     2581 catccgatga tcctgcagac gcatgagggc gactggcacc aggcggcacg gatctacagc
     2641 gcgtggttcc gctcgcaatt cagcgtcgtc gatccacgcg caagctggct ccaccgcgag
     2701 caggtctatc aggacgtcat ctcgctcctg cccgagggca acgtccacct gcggttccgc
     2761 gacatccccc aattcgccca ggatggactc gattacggcg tcaaggcgct gatcgtcagc
     2821 agttacaacg tcggcggcca cgacgcgaaa attcccagct acgacgatcc cgatccgcgt
     2881 ctggggacgt gggaggagtt gaaggccgcc atcgccgcct gccacgagag gggcgtcaag
     2941 gtcttcttct tcgtcaacct gcaaccggtg gacatggcgc tggattggta cgaacaggaa
     3001 ctgaagaact atgtcgtcca ggatcgttgg ggcattcatg cgatgcacat gggctggggc
     3061 atgggcacgc tcggcgcccg gctcggttat acccgccgcc cattggttcc ctgcaacccc
     3121 ggcatcgccc ggttccgtga aatcatcgtg cgccagctcg tgcacctcgc cgaaatcggc
     3181 gccgacggcg ttcatttcga caaactcggc tggccgatcg gcctcgattt caatccgctc
     3241 agtgacttca ccccggaccg cgggccggcc gagggcattc tattggcctt acgcgaaacc
     3301 ctcgacgcct gccgccggat caatccacac ttcgtcatct cctgcgaata ctggtgggac
     3361 cgcctgctgg aattcgccac cgtcgcctgg gcctggcaca cccagcttga tcacgaaccg
     3421 gcgctcaaat tcaccttccc cgaatggatg ccgatgatgc cggtcgtgca gccctatgcc
     3481 tggaacgtcg tgaacaacgc cacgcgctac ggctatcagg tgatggtcgg cgcggcgcgg
     3541 ttttcgcgct cgatgcagga cccgccaatg cgccccctct cgcaatacat cgcggaggtt
     3601 ctccgcatcc gcgaggagct cgtggatttc attcaattcg gcgaattcct cgacacgcag
     3661 gacgcgcgcg tcgaagcgca cgaggatgtg cgcttcaacg tccaccggca tcgcgagagc
     3721 ggtaagcgcg cctgcgtcat cgtcaatctg ggcctcaagg atcgcccggt gaagctgcgg
     3781 ctgaaaggaa atcacaaggg ccgcgtggag ctgcatgcgc cgttcaagaa aaagaagacc
     3841 gcgaacctgc cgcttcaagt caaactgccc ccgcgcggcc tgctgatcgc cgtcgagcaa
     3901 tgagccgtgc cccgctcggc cgcggggtgc gtttcttaat ggcattctgg aacgggggca
     3961 gccacgacgc tggaaaaccc cagcgagccc cctgccgggt gattttcaaa gggagagcca
     4021 cgttgtcacg cttgcgaccg ttcgccgcgc ttctgctgct ccccattttg aggccggccg
     4081 ccgtctccac cgccgcgcct gaacccatgg gctatctaca tgagggaagc ccggtcaccg
     4141 aacttccgat tcagctcagc cgcggcaaat atcgaaaaac cgacctcggc tatcgcgcgg
     4201 acgaagcctc gacgatcacg tccctcgagg tgcgtttcgc ggacctcacc ggcggtgatt
     4261 cgcttgcgtc aaaggagacc ggcattcagt ttttcgccgc ccttgacgcg ggcaatgcgc
     4321 gaccgctcgt accggcgatc ccccttgccg ttgccctgtc acccggccag ctcctcccgc
     4381 tgacgctctg gatcagaccg ggcgaccaga tcgacgaggg cccatttacc gcccaactgc
     4441 gcatgggcac ttcccatggg gaggaatccc tgccgctttc cctgtcgatc caccccggct
     4501 tgacgcccga tgcgggctgg cccgaatggc gctatgacga gcgacggatg gcgcgctcgc
     4561 cgctgaaggg ccgcatcgat caacccgtca ccaaatggga aatcccgctc ggcgcttcaa
     4621 gccagcccga agtgctgaac cagacccttg gctattccat tccacccgtc ccggcctggg
     4681 tcgccgccgg ggcgacggcc cgtgtcgcgg atctcgatcc cgacaatccg ggactcgagg
     4741 tcgcggtgtt tcatggcatc agccggctcg gcgaattgtt cacctatcag ggcgaactcc
     4801 tctggtccgt cactgccccg cgcgaaatgt atacgccgat cctgcaattt gccgatgtgg
     4861 atcaggatgg ccttctggaa attatagtca cgtcgcatgg gggcgtctcg gtctgggatg
     4921 gcgccaccgg ccatctcgac tacgacatcc cctatcaagt cggccggcaa tatgggcatt
     4981 cggtcgcggt caatatcgac gctgatcc
//
LOCUS       DAOPCO010000295         5005 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14160_length_5005_cov_5.008687, whole genome shotgun sequence.
ACCESSION   DAOPCO010000295 DAOPCO010000000
VERSION     DAOPCO010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14160_length_5005_cov_5.008687"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLS90_15035"
     CDS             <1..259
                     /locus_tag="PLS90_15035"
                     /inference="COORDINATES: protein motif:HMM:NF037222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03760.1"
                     /translation="GEDGNFRLGVRNTGDVPAFLAEVHVGARALASFIPSDNYFWLPA
                     GEEKWITLEKSPAASLALDPAAVTVRAWNAAPAPCQPPPGR"
     gene            900..3719
                     /locus_tag="PLS90_15040"
     CDS             900..3719
                     /locus_tag="PLS90_15040"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK03761.1"
                     /translation="MTSRLSPRAFAIACLALLALPPSLTAEVRPAMWTQYRGDRQLTG
                     RATGVGSFQTAVQPEWSVRISGREGYVEIDPGLPANELALPDPNSTGSGYLNPANQIM
                     WQVGPPALDLYGTGANVQIAEGPTFKVAKILPDVQGLQRFEMQTYYTHENSARGTLYS
                     YQNGSAQQVWQTPPVDNAWGPVAIVTDVNLDGQPDIAMSSHYRLIFIDGATGQFLHPI
                     DGSLADPGNSRYHNARNYGWFGSANIDDDPYPEFCVISDFASHIEVLDWDGSQFHQLW
                     RKEIENVIVRNKKVVRPGPAAFLDLDADGDVEVFLNLYDHTGDKQWHFVAYDAWTGNV
                     QYDFPQRYLEGVGDLNGDGLYELFVSDSAGLATPTYAPVRILNLNPAAPASPTQLFHD
                     PYAKWHARDVDQMPLTANTMAADGRRTVVLRDANDDGDLDFFLSRPDPVTGVETAFAY
                     DLNGGSPSLIWSIQGAPGMQLNTIAAANVDADGRTESLLHLTGRAGPGQLLATLQGGA
                     ALHMWAYSTTNTGTPVVADLENDGIVEVIVQTGNEEILCLEAPRYVTPGAAPRERWRV
                     RGRGMTNSAPLYHGVSAADVDGDGDLEVLLSQEADNGQASLVAVDPDGSILWRCVFPD
                     FDGSPPIWNFGGLTFWTAGHFRTLGHMDVYVSLRRSTMHSDVGFLVDGLNGQILWERD
                     GIWPANSNNEEDGRGHGGVAVACGNLAGDGGLDKLVVEFPDRYYVVDGISGDPDLVVR
                     TDGTTSIFGGYWVAYAKPIIADLFGNAVPEIVWGACAYLTAVMTPQGVPFWNSPYIVA
                     GSPPVPLQAIADVDGNGSPELVGVYGSSVSVQNPANGDTLYTRSVTTPTRDLVSGDLD
                     NDGRDEVLITTNSAIRCFELVGTEFRQTWVLSAGGAATDPAIADVDADGLLEIVVATS
                     SGQLLMFDSPDPAFETGLAHWPDYH"
     gene            complement(4081..>5005)
                     /locus_tag="PLS90_15045"
     CDS             complement(4081..>5005)
                     /locus_tag="PLS90_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPK03762.1"
                     /translation="ELFGRKLKEMGLDAGVTKRVLSRVPDEFTLAHLLHAVDVARASD
                     AMLPQYETVFSTMIWVARANYQLQLAPGESISDLILFPHSQSEARGIEDLRLVRYVDT
                     DGSARYYGTYTAFDGQNILPMLLETEDFRTISIHTLNGACVQNKGMALFPRRIDGHYV
                     MCSRIDGRNLYLMYSDYVHFWETARLLAAPKYPWELRLIGNCGSPLETPAGWLLLTHG
                     VGPMRRYCIGAMLLDLKDPFRIIGRLREPLLAPSVEEREGHVPNVVYSCGGMIHGGKL
                     IIPYAFSDTATRIALVEVDELLERLVKDGAG"
BASE COUNT          843 a         1997 c         1249 g          916 t
ORIGIN      
        1 cggcgaggac ggcaacttcc gcctcggcgt gcgcaacacc ggcgacgtgc ccgccttcct
       61 cgccgaagtc cacgtcggcg cccgggcgct cgccagcttc atcccgagcg acaactactt
      121 ctggctcccc gccggcgagg aaaagtggat cacgctcgaa aaaagcccgg ccgccagcct
      181 cgccctcgat cccgccgccg tcaccgtccg cgcttggaac gccgcccccg ccccttgcca
      241 acccccgccc ggccggtgac acttcgattg tgctcagcgg gggtgacatt ccggcgtggg
      301 tccggcgccc ccgccggtca ttccctccca cgcttcccgc agggatgccg gacgatatgg
      361 tcccggcccc cgatcttcca tgaacgcctg ccccgcgtag cccgcgccag cgggcttttt
      421 ccgggccccc tcgggggccc gaacattggc gccgcgcctc ggcctggtga ccccacgcga
      481 cagattcttc tcaaccgttt caacggtttc cccgccaacg ccgccatccc ggtgtccgac
      541 tcatcggttc tcactgaatg ccttatgcct tcgcgcatca actcctctat gcctttgcgt
      601 cttcgcggac attcctcttt gtctgcacca tttccatttt cttgaattcg atcctccgaa
      661 tttgtcttaa ttttcacact ttgactttca tgcttctcct cgctcacttt cccccacttc
      721 ctgctcccct ctatctccta tctcctcttc cctatctcct cgcccccatc ctctcgcctc
      781 ttcctcctcc cacggttgac gtattcccca ctacatccct atactcatct gcaggaacgc
      841 ggcgtttacc cgtacataac cgctcatggc ggatcatttc gccgacaagg tggcctgcaa
      901 tgacttcccg gctcagcccc cgtgcgttcg ccatcgcctg cctcgctctg ctcgcccttc
      961 ctccctcgct caccgccgaa gtccgacccg ccatgtggac ccagtatcgc ggcgaccgcc
     1021 aactgaccgg ccgtgccacc ggcgtcggca gcttccagac cgccgtccag cccgaatggt
     1081 ccgtccgaat ctccggccgc gaaggctatg tcgaaatcga ccccggcctg cccgccaatg
     1141 aactcgccct ccccgacccc aactcgaccg gctccggcta cctcaacccc gccaatcaga
     1201 tcatgtggca ggtcggtccc cccgcccttg atctctacgg caccggcgcg aacgtgcaga
     1261 tcgccgaggg accaactttc aaggtcgcca aaatcctccc cgatgtgcag ggcctccagc
     1321 gcttcgaaat gcaaacctac tacacccatg agaacagcgc ccgcggcacg ctctacagct
     1381 accagaacgg cagcgctcaa caggtgtggc agacccctcc cgtcgacaac gcctggggcc
     1441 ccgtcgccat cgtcaccgac gtcaacctcg acgggcagcc cgacatcgcc atgtcctccc
     1501 actaccggct catcttcatc gacggcgcca ccggccagtt cctccatccc atcgacggct
     1561 cgctcgccga ccccggcaac tcccgctatc acaacgcccg caactacggc tggttcggct
     1621 cggccaacat cgacgacgac ccctaccccg agttctgcgt catcagcgat ttcgccagcc
     1681 acatcgaggt cctcgactgg gacggttcgc agttccacca gctctggcgt aaggaaatcg
     1741 aaaacgtcat cgtccgcaat aagaaggtcg tccgcccggg acccgccgcc ttcctcgacc
     1801 tggacgccga cggcgacgtc gaagtcttcc tcaacctcta cgatcacacc ggcgacaaac
     1861 agtggcactt cgtcgcctac gacgcctgga ccggcaacgt ccaatacgat ttcccccagc
     1921 gctatctgga gggcgtcggc gacctgaacg gcgacggcct ctacgaactc ttcgtctccg
     1981 actccgccgg cctcgccacc cccacctacg ctcccgtgcg catcctcaac ctcaatcccg
     2041 ccgcgccggc cagccctaca cagctcttcc atgatcccta cgccaagtgg cacgcccgcg
     2101 acgtcgatca gatgccgctg acggccaata cgatggccgc cgacggccgc cgcacggtcg
     2161 tcctgcgcga cgccaacgac gacggcgacc tcgacttctt tctgagccgg cccgaccccg
     2221 tcaccggcgt cgagaccgcc ttcgcctacg acctcaacgg cggctcgccc tcccttatct
     2281 ggtcgatcca gggcgccccc ggcatgcagc tcaacaccat cgccgcggcc aacgtcgacg
     2341 ccgacggccg caccgaatcg ctcctccacc tcaccggacg tgccggccct ggccagctcc
     2401 tcgccaccct ccagggcggc gccgccttgc acatgtgggc ctactccacc accaacaccg
     2461 gcacccccgt cgtcgccgac ctcgagaacg acggcatcgt cgaggtcatc gtccagaccg
     2521 gcaatgagga gatcctctgc ctcgaagccc cgcgctacgt cacccccggc gccgcccccc
     2581 gcgagcgctg gcgcgtccgc ggccgcggca tgaccaattc cgcccccctc taccacggcg
     2641 tcagcgccgc cgacgtcgac ggcgacggcg atctcgaagt cctcctctcg caggaggccg
     2701 acaacggtca ggccagcctc gtcgccgtcg atcccgacgg ctcgatcctc tggcgctgcg
     2761 tcttccccga cttcgatggc tcccccccga tctggaactt cggcggcctg accttctgga
     2821 ccgccggcca cttccgcaca cttggccaca tggacgtcta cgtctccctg cgccgcagca
     2881 ccatgcacag cgacgtcggc ttcctcgtcg acggcctcaa cggtcagatc ctctgggaac
     2941 gcgacggaat ctggcccgcc aacagcaaca atgaagaaga tggccgcggc catggaggcg
     3001 tcgccgtcgc ctgcggcaat ctggccggcg acggcggcct cgataagctt gtcgtcgaat
     3061 tccccgatcg ctattacgtc gtcgacggca tcagcggcga tcccgacctc gtcgtgcgca
     3121 ccgacggcac cacctcgatc ttcggcggct attgggttgc ttatgccaag ccgatcatcg
     3181 ccgatctgtt cggcaatgcc gtccccgaaa tcgtctgggg cgcctgcgcc tacctgaccg
     3241 ccgtcatgac gccccagggc gtccccttct ggaacagccc ctatatcgtc gccggctcgc
     3301 cccccgtccc gctgcaggcc atcgccgacg tcgacggcaa cggctcgccc gaactcgtcg
     3361 gcgtctacgg ctcatcagtc agcgttcaga atcctgccaa tggcgacacc ctctacaccc
     3421 ggtcggtcac gacccccacg cgcgatcttg tctccggcga tctggacaac gacggccgcg
     3481 acgaggtcct gatcacgacc aactccgcca tccgctgctt cgaactggtc ggcaccgaat
     3541 tccgccagac ctgggtcctc tcggcggggg gcgccgccac cgaccccgcc atcgccgacg
     3601 tcgacgccga cggcctcctc gaaatcgtcg tcgccacctc ttccggtcag ctcctcatgt
     3661 tcgacagccc cgatcccgcc ttcgaaaccg gtctggccca ctggcccgac taccactgac
     3721 ccacgcgccg actacggctt ccacaccccg cactccagcg tataccaggc cgagcttgcg
     3781 ctattcggca agctcgagct ccctgttcag cgtgcagcgc tgaatcgttg catcgcaacg
     3841 cctcctgctt ggagtgcggc ggcagagcgt tgcatcgcaa cgcgtcgaca ccgctttgga
     3901 tcgaatggcc gaatccgctt ccgatcatat ctccgcgttc atatgtttca ggtgctcccc
     3961 aatttaacct tcgttcaatt cctccagatt tccccctctc ccgacttact ccatttcccc
     4021 tttctcaact gctttcattc gattttgttt cgaatttcga tattcgcatt tcgtgtttcc
     4081 tcaccccgct ccatccttca ccagccgctc cagcagctcg tccacttcca ccagcgcgat
     4141 gcgggtcgcc gtatccgaaa acgcgtacgg aataatcagc ttgcccccgt ggatcatccc
     4201 cccgcaggaa tagacgacat tcggcacgtg cccttcccgt tcctccaccg acggcgccag
     4261 caacggctcg cgcaggcgcc cgataatccg aaacgggtcc ttcagatcga gcagcatcgc
     4321 cccgatgcag tagcgccgca tcggccccac cccgtgcgtc agcagcagcc agccggccgg
     4381 cgtctccagc ggcgacccgc aattgccgat cagccgcagc tcccacgggt atttcggcgc
     4441 ggccagcagc ctcgccgttt cccagaagtg aacataatcg gaatacatca gatacagatt
     4501 gcgcccgtcg atccgcgaac acatcacgta gtggccgtcg atccgccgcg gaaacagcgc
     4561 catccccttg ttctgcacgc aggcgccgtt gagcgtgtgg atcgagatcg tgcgaaaatc
     4621 ctcggtctcc agcagcatcg gcaggatgtt ctgcccgtcg aacgccgtgt acgtcccgta
     4681 gtaccgcgcc gacccgtccg tatcgacata ccgcaccagc cgcagatcct cgatcccgcg
     4741 cgcctcgctc tggctgtgcg gaaacaggat caggtcggag atcgattcgc ccggcgccag
     4801 ttgcaattgg taattcgccc gcgccaccca gatcatcgtc gaaaacaccg tctcgtattg
     4861 cggcagcatc gcgtcgctgg cccgcgcgac atccaccgca tgcagcaggt gggctagcgt
     4921 gaactcgtcg ggcacccgcg acagcacccg tttcgtcacc cccgcatcca gacccatttc
     4981 cttcagtttg cgcccgaaca gctcc
//
LOCUS       DAOPCO010000296         4992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14200_length_4992_cov_5.300385, whole genome shotgun sequence.
ACCESSION   DAOPCO010000296 DAOPCO010000000
VERSION     DAOPCO010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14200_length_4992_cov_5.300385"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1326)
                     /locus_tag="PLS90_15050"
     CDS             complement(49..1326)
                     /locus_tag="PLS90_15050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK03763.1"
                     /translation="MPLTFPASAPPLLFLSPRVPWPLNTGAKIRTHALLRALCEAFPV
                     HYAGFLQPDLNADEARRRLGACETIKLYPEQGISLRGKAWLACRALADSRPVNILKYD
                     HGGLRDYVSLWIQENPAGLVHADHLHMAACLGDASPVACVIDEHNVESVILERMASSY
                     HRRGLRPWLRRQARRMADFEARTIARRAQALAVSPEDAARLEALAPGARVTVVPNGVD
                     LDYFTPPPPDNRRIAGRLVFTGSMDWLPNQDAVMHFLDSIWPRLPRTPGQPPWQFDVV
                     GRSPSAGLRARSDAAIRVTGAVPDIRPFLHEAMMLVVPLRIGGGSRLKILEAFAAGLP
                     VVSSRVGCEGLEVEDGRHLLIADEPEAFAAAIARLHGDEALRHELAAAGRRLAQLRYD
                     WRTIGRDLVQTYQNLISAELAATPTPVEAGTAG"
     gene            complement(1317..1754)
                     /locus_tag="PLS90_15055"
     CDS             complement(1317..1754)
                     /locus_tag="PLS90_15055"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RuvX/YqgF family protein"
                     /protein_id="HPK03764.1"
                     /translation="MDPMHPAPPTWDELLGQTLLGIDYGSRRIGVAIKPAGQDWVLPK
                     TVIQVESEPAAAAALQELIAREKAEGVVVGLPEHPDTRQARDVKRFCRRVRRDLTGVR
                     WFFVDERFTTEAAHSISIDRPRRRPSDELAATLILETFLQRCR"
     gene            complement(1832..3901)
                     /locus_tag="PLS90_15060"
     CDS             complement(1832..3901)
                     /locus_tag="PLS90_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03765.1"
                     /translation="MTASPHKPDPAASRRCLFQSGLLLFTLLLITLGLWQASNRFVGE
                     RQEDALRQRGLPIIQEFDHFMSERVNALRQVANFYAYSSEVRAEEFLGFTSSVMSEAP
                     GIVAILSTDREGIPIWASPPDALTPGDINWLTADPKLTQALDRARLSLQPAATELLDL
                     PEYGEWFLVAAPVITRGQHLGFIVGIFQYQRLIQYLLRPDLFQDYRISIFHANRPVHT
                     AFTPHGVIRANEDAPGAVSQTRNLAGQPLEIIVEPTASRRFAELNITSLIIVSLGVVL
                     SLLLSFLFHRWQWRTLKLQSEAAERLNRLERTGLDLAEVRGELELILNSVDEGIIFYN
                     AQLEPVQANPSFLAMFNMTERGRYMSRGGTHHEHMIQFLGSESKYWALFENLRRNPGQ
                     TYADELEVPARVEGRVNRTYVRRATLIHATDGRQRGVLAIYKDVTSLVAMDRVKDEFL
                     SNVTHELRSPLASIKGFAETLRRDQAMPAETRDEFLSIIYEESTRLQDLIEELLDLRR
                     LEARGVTLNPVAFDFRILVDDVIRSMRSVFFSKKVAATVEWTGRSEGMLRGDVPQLTR
                     ALRNLLVNAVKYSPEGGRISVRGHLGTQQVWVEIQDQGVGIAEADLPHIFDKFYRGMG
                     RGRQKGTGLGLAIVKHTIELHGGHLGVRSEVGAGTTFRIELPRQFSPPVTAKIVGAGA
                     EMDAVGL"
     gene            complement(4260..>4992)
                     /locus_tag="PLS90_15065"
     CDS             complement(4260..>4992)
                     /locus_tag="PLS90_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HPK03766.1"
                     /translation="LESGESVIQETRLWDEPSGRTRAMRSKEVAHDYRYFPDPDLVEI
                     HVDEAWVGRVRAELPELPDARRRRFVEQYGLTDYSAGVLTARKPVAEYFESALRSHAN
                     PVGIGNWVINEVLRWLGEDPDRELADYPVAAHDLAVLVRMIDEATISGKIAKEVYKAM
                     MAGEGAPEAIVKAHGLVQITDTSEIEPIVARVVAENPDTVAKIRAGKDKAIMALVGQV
                     MKETRGKANPQTVNELIRSRIDEMV"
BASE COUNT          828 a         1566 c         1813 g          785 t
ORIGIN      
        1 ccccggccca gtttcatggt gaattttact ttatgcatca agaaaaagtc aacctgcggt
       61 tccggcttcg acgggggtcg gggtggcggc caactccgct gaaatcagat tctgataggt
      121 ctgcacgaga tcgcggccga tggtccgcca gtcgtagcgc aactgcgcca ggcgccggcc
      181 ggccgcggcc agttcatggc gcagcgcctc gtcgccgtgc agccgggcaa tcgccgcggc
      241 aaaggcctcc ggctcgtcgg cgatcagcag atggcggccg tcctccacct cgagcccctc
      301 gcagccgacg cggcttgaaa cgaccggcaa tccggcggca aaggcctcga ggatcttcag
      361 ccggctgccg ccgccgatcc gcagcgggac gaccagcatc atcgcttcgt ggaggaaggg
      421 gcgaatgtcc ggaacggcgc cggtgacgcg aatcgccgcg tcgctccggg cgcgcaggcc
      481 ggccgagggg gaccggccga cgacgtcgaa ctgccatggc ggctggccgg gcgtgcgggg
      541 cagccggggc cagatcgaat cgaggaaatg catgacggcg tcctgattgg gcagccagtc
      601 catcgatccg gtgaacacaa gccggcccgc gatgcggcgg ttgtccggcg gcgggggcgt
      661 gaagtagtcg agatcgacgc cgttggggac gaccgtgacg cgggcgccgg gggccagggc
      721 ttccaggcgc gccgcgtcct cgggcgagac ggcgagcgcc tgggcgcggc gggcgatcgt
      781 ccgcgcctcg aagtcggcca tccggcgcgc ctggcgccgc agccacggac gcaggccgcg
      841 gcggtgatag ctggaggcca tccgctccag gatcacgctt tcgacgttgt gttcatcgat
      901 cacgcaggcg accggcgacg catcgcccag acacgcggcc atgtggagat gatcggcgtg
      961 gacgagcccg gcgggattct cctgaatcca cagcgagacg tagtcgcgca agcccccatg
     1021 gtcatatttc aggatgttga ccgggcgcga gtcggcgagg gcgcggcagg cgagccaggc
     1081 cttgccgcgg agggagatcc cctgctcggg gtaaagcttg atggtttcgc aggcgccgag
     1141 gcggcgccgc gcttcgtccg cattgaggtc gggttgcaga aaaccggcgt agtgcacggg
     1201 aaacgcctcg cacagcgcgc gcagcaaggc gtgcgtgcgg atcttggcgc cggtgttgag
     1261 cggccaggga acgcgcggac tgagaaacag cagcggcggc gcggaagcgg ggaaggtcag
     1321 cggcatcgct gcagaaaggt ctccaggatc aaagtggcgg cgagttcgtc ggacgggcgg
     1381 cgacgcggcc tatcgatact tatcgaatgg gcggcctcgg tggtgaagcg ctcgtcgacg
     1441 aaaaaccagc gtacaccggt caggtcgcgg cggacgcggc ggcagaagcg ctttacgtcg
     1501 cgcgcctggc gcgtgtcggg atgctccggc aggccgacga cgaccccctc ggccttctcg
     1561 cgggcgatca gctcctgcag ggcggcggcc gcggccggtt cgctttcgac ctggatcacg
     1621 gtcttgggca gaacccagtc ctggccggcc ggtttgatgg cgacgccgat gcgccggctg
     1681 ccatagtcaa tgcccagcag cgtctggccg agcagctcgt cccacgtcgg cggcgcgggg
     1741 tgcatgggat ccatcgggcg cggctccccc ctgttggaat ggctcgccgg cggcggcatc
     1801 cgccgctcct cccggcgcgg gcgggcgggc gtcacaggcc gacggcatcc atctcggcgc
     1861 cggcccccac gatcttcgcc gtcacgggcg gggaaaactg ccgcggcagc tcgatgcgga
     1921 acgtcgtccc ggcgccgacc tcgctgcgga cgcctaagtg gccgccatga agctcgatcg
     1981 tgtgtttgac gatcgccagc cccaggccgg tccctttctg ccgcccgcgg cccatcccgc
     2041 ggtagaactt gtcgaagatg tgcggcagat cggcttcggc gatgccgacc ccctgatcct
     2101 ggatctcgac ccagacctgc tgcgtgccga gatgaccgcg aacggagatc cgccccccct
     2161 cgggggagta cttgacggcg ttgaccagca ggttgcgcag cgcgcgggtc agctggggga
     2221 cgtcgccgcg gagcatcccc tcgctgcgac cggtccactc caccgtcgcc gccaccttct
     2281 tggagaagaa gaccgagcgc atcgagcgga tgacatcgtc gacgaggatg cggaagtcga
     2341 aggcgacggg gttaagggtg acgccgcgcg cctcgagccg gcgcaggtcg agcaattcct
     2401 cgatcagatc ctggaggcgg gtcgactcct cgtaaatgat cgagaggaat tcatcgcggg
     2461 tttcggcggg catcgcctga tcgcgccgca gcgtctcggc gaagcccttg atcgaggcga
     2521 gcggggagcg cagctcatgg gtgacgttgg agaggaactc atccttgacg cggtccatcg
     2581 ccaccagcga ggtgacgtcc ttgtagatgg cgaggacgcc gcgttgccgg ccgtcggtgg
     2641 cgtggatcag cgtggcgcgg cggacgtagg tgcggttgac gcggccttcg acgcgcgcgg
     2701 ggacttcaag ttcgtcggcg taggtctgac ccggattgcg gcgcagattc tcaaacaatg
     2761 cccagtattt ggactccgag ccgaggaact ggatcatgtg ctcgtggtgc gtgccgccgc
     2821 gcgacatgta gcgcccgcgc tcggtcatat tgaacatggc caggaacgac gggttggcct
     2881 gaaccggctc caactgggca ttgtagaaga tgatgccctc atccacgctg ttgaggatca
     2941 gttccagctc gccgcggacc tcggccaggt ccaggccggt ccgttccaac cggttgaggc
     3001 gctcggcggc ttcggactga agtttgagcg tgcgccactg ccagcggtgg aacaggaagc
     3061 tgagcaggag cgagaggacc acgccgagcg aaacgatgat cagcgaggtg atgttcaatt
     3121 cggcgaaccg acgcgaggcg gtcggctcga cgatgatttc cagcggctgg ccggccaggt
     3181 tgcgcgtctg gctgacggcg ccgggcgcat cctcgttggc ccggatgacg ccgtggggcg
     3241 tgaaggcggt gtggacggga cggttcgcgt ggaagatgct gatgcgatag tcctgaaaca
     3301 ggtccggacg caacagatac tgtatcaggc gctggtattg gaagatgccg acgatgaagc
     3361 cgagatgctg gccgcgggtg atgacggggg cggcgacgag gaaccattcg ccatactcgg
     3421 gcagatcgag caattcggtg gccgccggct gcaggctgag ccgcgcccgg tcgagcgcct
     3481 gggtgagctt cggatcggcc gtcagccagt tgatgtcgcc cggcgtcagg gcgtcggggg
     3541 gggaggccca gatcgggatc ccttcgcggt cggtgctcag gatcgcgacg atgcccggcg
     3601 cctcggacat gaccgacgag gtgaagccga ggaactcctc ggcgcgcacc tcgctggagt
     3661 aggcgtagaa gttcgccacc tggcgcagcg cgttgacccg ctccgacatg aaatggtcga
     3721 attcctggat gatcggcagg ccgcgctggc gcaaggcgtc ctcctgccgc tcgccgacga
     3781 agcggttgga tgcctgccac agccccagcg tgatcagcag cagggtgaac aggagcagtc
     3841 cggactgaaa caggcaacgg cggctggccg cgggatcggg cttgtgcgga gaagcggtca
     3901 tgaaaagcgg cggcggcgcg ccggcgaatg cacgggtgag atgggcccgc gccgcgcccg
     3961 gcggaggcga accaacctaa ttgtaaggac caatgccccg cgcgcgcaag gattcgccgc
     4021 ccggcgggga tgggagcggg ccggccgggg cgcgaaccgg acgatgagcg tcagagttca
     4081 cggagttcat ggggaagttt gcaggggaaa ccgttgaaag gaacgaatgg gcgggtttcg
     4141 aaagccgagg acgagggacg agggccgaga gccggattct ggagaggacc tttgggagcg
     4201 atcccagggg acgagggcga gaaacgaaga cgagagacga ctctcgggtg aggggcgagt
     4261 cagaccatct cgtcgatcct gcttcggatc agttcgttga ccgtctgcgg gttggccttg
     4321 ccgcgcgtct ccttcatcac ctggccgacg agcgccatga tcgccttgtc cttgccggcg
     4381 cggattttcg ccaccgtatc ggggttttcg gccaccacgc gcgcgacgat cggctcaatc
     4441 tcgctggtgt cggtgatctg caccaggccg tgcgccttga cgatcgcctc gggggcgccc
     4501 tcgccggcca tcatcgcctt gtagacctcc ttggcgatct tgccgctgat ggtcgcctcg
     4561 tcgatcatgc ggaccagcac cgccaggtcg tgcgccgcga ccggatagtc ggccagctcg
     4621 cggtcggggt cctcgcccag ccagcggagc acttcattga tcacccagtt gccgatgccg
     4681 accgggttgg cgtggctccg cagcgccgat tcgaagtatt cggccaccgg cttgcgggcg
     4741 gtcaggacgc cggcgctgta atcggtcagg ccgtattgct ccacgaaccg gcgccggcgc
     4801 gcgtcgggca attcgggcag ttcggcccgg acgcggccga cccacgcctc gtccacatgg
     4861 atctccacca gatcggggtc ggggaagtag cggtaatcgt gcgccacttc cttcgagcgc
     4921 atcgcgcgcg tccggccgct cggctcatcc cacaggcgcg tttcctggat gacggactcg
     4981 cccgactcga gc
//
LOCUS       DAOPCO010000297         4982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14230_length_4982_cov_5.584331, whole genome shotgun sequence.
ACCESSION   DAOPCO010000297 DAOPCO010000000
VERSION     DAOPCO010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_4982_cov_5.584331"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLS90_15070"
     CDS             <1..744
                     /locus_tag="PLS90_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPK03767.1"
                     /translation="PTCLGLCYNKALVRAIALSLEIPVPLETYFNPDDQSATLPSTFP
                     ALLKPNFGDSSQGITQHAVVHSPEQLISYLRNLQEELPGRPILFQEFLSGKEYSIGII
                     GNPGLALRFLPALEVDYSGLPAELPPILGYESKWDPTSPYWGAIRYHEARLPEEELRK
                     LQDYSSLLFERLGCRDYARFDWRADANGEIKLLEVNPNPGWCWDGKMNIMTGFMGLRY
                     CDFLRMILEAAQERVGATVANGQLTPAGV"
     gene            987..2393
                     /gene="murD"
                     /locus_tag="PLS90_15075"
     CDS             987..2393
                     /gene="murD"
                     /locus_tag="PLS90_15075"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPK03768.1"
                     /translation="MTTPAFLQYEHYAVLGAGASGRSTSRLLLDRGKRVTLFDDYADP
                     AAGNLQPLRERGAALQFGPPQTTAAALAQVQACVVSPGIPIDHPFIAEARRRGKAVLA
                     EIELGWLLRGGARVAAITGTNGKTTVTMLLQRLLEEGGLDSIAAGNIGLPMTEAVTAR
                     AGEALARTIFAVEISSFQLETTDAFAPEVAVILNVTPDHLDRHGSMEAYTRAKARITG
                     RQRADHVLVVNQDDAACLAIAAASRARIRRFSLERPVQDGAWLDGELIMIIEPGQRPR
                     RLIAIDELKMVGLHNVANAMAAACAAQVLGVGREAIAATLAAFRSAPHRMEIVATRDG
                     VTYINDSKATNLDAMVQAVSSFEGGIHLIAGGRDKASPFDTITHHLERRVRRLYLIGE
                     AAADMEQAWGERIECRQCGTLANALSEATAAAADGEIILLSPGCASFDQFQSYADRGR
                     QFVEWVNALPPNPVPRPS"
     gene            complement(2492..3610)
                     /locus_tag="PLS90_15080"
     CDS             complement(2492..3610)
                     /locus_tag="PLS90_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03769.1"
                     /translation="MIVWYITSHGFGHAVRSTLILARVDREIPVTLRTECPRWFLEQK
                     LMGRPFELAPAAFDCGTLGPDATRIDLRRTLERAEELIARNERRIDEEVQWLRARGAR
                     LVVADIVPFALRVARRAGIPSILIANFTWAGIYRHLVEMEQPDEALRRRAAAVIEHMR
                     AEYAEGDLLLTTGFVSDAEACRERREMPLVAAAGRPRRAELARARGLDPARPIYLVYL
                     GREGLQGLEPDARPRLRDLQLVGYAAPAGLEELVRPLDEETMPTADAFASVDGVIGKA
                     GYGTCAQCLAVGTRMLYLPRPQFAEWYALDRALRRWGGGLCIPERDFLRLDWRPWLER
                     LGRQRIRQTPDCTGAERCAREIERAWREGLSGKEREGP"
     gene            3857..4408
                     /locus_tag="PLS90_15085"
     CDS             3857..4408
                     /locus_tag="PLS90_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK03770.1"
                     /translation="MALTYSTMMELGTTAPDFSLPGTDGQLWRRDDAKGTRGTLIVFT
                     CNHCPYAKAVEDRLIAIGRDYQPRGIGIVLINSNDADNYPEDSFDKMKERAAARNYPF
                     PYLFDESQDVARAYHAACTPDPFLFDRHLRLYYRGRIDDNWQDPDKVRRHDLRDALDL
                     LLADKPAPVEQLPSMGCNIKWKE"
     gene            4459..>4982
                     /locus_tag="PLS90_15090"
     CDS             4459..>4982
                     /locus_tag="PLS90_15090"
                     /inference="COORDINATES: protein motif:HMM:NF013992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMO1 family protein"
                     /protein_id="HPK03771.1"
                     /translation="MTFPPVRYVQAVPVMHDGQHVVVLQDPEGLCPETLAVPLPLYLI
                     MTLLDGRRDARAVQTEIARMAQGQIVPTQEIERIVHELDELYLLENARARERRAELER
                     EFDALPLRPPIHAGAAYPAEADALQEMMRQLFDGLTPAAADAPRPRGLILPHIDLRVG
                     GRALARALATLDADR"
BASE COUNT          791 a         2004 c         1435 g          752 t
ORIGIN      
        1 ccgacctgcc tcggcctgtg ctacaacaag gccctcgtcc gcgccatcgc gctctcgctc
       61 gaaatccccg ttccgctcga aacctacttc aaccccgacg atcagtccgc gacgcttccc
      121 tccaccttcc cggccctgct caaacccaac ttcggcgaca gcagccaggg catcacgcag
      181 cacgccgtcg tccattcccc cgaacagctc atctcctacc tgcgcaacct gcaggaggaa
      241 ctgcccggcc gcccgatcct gttccaggag ttcctctccg gcaaggaata cagcatcggc
      301 atcatcggca acccgggcct cgcgctgcgt ttcctgcccg ccctcgaagt cgattacagc
      361 ggcctgcccg ccgagttgcc tccgatcctc ggctacgaat ccaaatggga ccccacctcg
      421 ccctactggg gcgcgatcag gtatcacgag gcgcggctcc ccgaggagga actgcgcaag
      481 ctccaggatt actcaagcct gctgttcgaa cggctcggct gccgtgatta cgcccgcttc
      541 gactggcgcg ccgacgccaa tggcgaaatc aagctgctcg aggtcaaccc caatcccggc
      601 tggtgctggg acggcaagat gaacatcatg accggcttca tgggcctgcg ctactgcgac
      661 ttcctgcgca tgattctgga agccgcgcag gagcgcgtcg gcgcgaccgt cgccaacggc
      721 cagttgaccc cggcgggtgt ctagggccgc cagtgcgggc ccggcggtgt gggaccggcc
      781 agtgtaggac cggcgccccc gccggtccag ccccgcctcc acctcccggg cgctaacaga
      841 cacccaccgc ggcacttcag aatgccccgc gcttgagcta tcgtgcttct ggtgcgccgc
      901 gcgccggtca gccggatacc ggccaacgac cggatcgccc ccgccgcgcg gcgcgcacgc
      961 catccttccc cgcgagttgc ccggccgtga ccacccccgc ctttcttcaa tatgaacact
     1021 acgccgtgct cggcgcgggc gccagcggcc gctccacatc gcgcctgctg ctcgaccgcg
     1081 gcaagcgcgt cacgctcttc gacgactatg ccgatccggc cgccgggaac cttcaacccc
     1141 tgcgcgagcg cggcgcggcg cttcagttcg gccctcccca gacgaccgcg gcggccctcg
     1201 ctcaggtcca ggcctgcgtc gtctcgcccg ggattcccat cgaccatccg ttcatcgccg
     1261 aggcgcgccg ccgcggcaag gccgtgctgg ccgagatcga gttgggctgg ctgctgcgcg
     1321 gcggggcgcg cgtcgccgcc atcaccggca ccaacggcaa gaccaccgtc accatgctcc
     1381 tccagcgcct gctggaggaa ggcggcctcg attcaatcgc cgccggcaac atcggactgc
     1441 cgatgaccga agccgtgacc gcccgcgccg gcgaggccct cgcccgcacg atcttcgccg
     1501 tcgagatcag cagctttcag ctcgaaacga ccgacgcctt cgcccccgag gtcgccgtca
     1561 tcctcaacgt cacgccggat catttggacc gccacggcag catggaggcc tacacccgcg
     1621 ccaaggcccg catcaccggg cgccagcgcg ccgaccacgt cctcgtcgtc aatcaggacg
     1681 acgccgcctg cctggccatc gccgccgcca gccgcgcccg catccgccgc ttttcgctgg
     1741 agcgtcccgt ccaggacggc gcctggctcg atggcgaact gatcatgatc atcgagccgg
     1801 gccagcgccc gcgccgcctg atcgcaatcg acgagctcaa gatggtcggc ctgcacaatg
     1861 tcgccaatgc gatggcggcg gcgtgcgcgg cgcaggtcct cggcgtcggc cgcgaggcga
     1921 tcgccgccac gctcgcggcc ttccgctccg ccccccaccg gatggaaatc gtcgccacgc
     1981 gcgacggcgt cacttacatc aacgactcga aggccacgaa cctcgacgcg atggtccagg
     2041 ccgtcagtag cttcgaaggg ggcatccacc tgatcgccgg cggccgcgac aaggcgagcc
     2101 cgttcgatac gatcacgcac cacctggaac ggcgcgttcg ccggctctac ctgatcggcg
     2161 aggccgccgc cgacatggaa caagcctggg gcgagcggat cgagtgccgc cagtgcggaa
     2221 cactcgccaa cgcgctgagc gaagcgaccg ccgccgccgc ggacggcgaa atcatcctgc
     2281 tctcacccgg ctgcgccagc ttcgaccagt tccagagcta cgccgaccgc ggccgccagt
     2341 tcgtcgaatg ggtcaacgcc ctccccccaa atcccgtccc tcgtccctca taaaatcgtc
     2401 cctcgtccct cgtcctcgtt ctcggctctc gcaaatcgcc ctttcctttt tcgtgtcttt
     2461 cgtgcctttc gtaatctcat ttctcctctt ctcacggccc ctccctctct ttcccgctca
     2521 gcccctcgcg ccaggcgcgc tcgatttcgc gcgcgcagcg ttccgccccc gtgcaatccg
     2581 gcgtttgccg gatccgctga cggcccagcc gctccagcca gggccgccag tccagccgca
     2641 ggaaatcccg ctcggggatg cacagcccgc ccccccagcg ccgcagcgcg cggtccagcg
     2701 cataccattc ggcaaactgc ggccgcggca gatacagcat ccgcgtaccg accgccaggc
     2761 actgtgcgca cgtcccgtaa cccgccttgc cgatgacccc gtccaccgag gcgaacgcgt
     2821 cggccgtggg catcgtctcc tcgtccagcg ggcgcaccag ctcctccagc cccgccggcg
     2881 ccgcgtaacc gaccagttgc agatcgcgca gccgcgggcg cgcatccggc tcaagcccct
     2941 gcaggccctc ccgtcccagg taaaccagat aaatcggccg cgccggatcg agcccccggg
     3001 cgcgcgccag ttctgcccgg cgcgggcggc ccgccgccgc caccagcggc atttcgcgcc
     3061 gctcccggca cgcctccgca tcgctcacga atccggtcgt cagcagcaga tccccctcgg
     3121 catattccgc gcgcatgtgc tcgatgaccg ccgccgcgcg ccgccgcagc gcctcgtccg
     3181 gctgctccat ctccaccaga tgccggtaaa tgccggccca cgtgaagttg gcgatcagga
     3241 tcgacgggat gcccgcccgc cgcgcgaccc gcaaggcgaa cggcacgata tcggccacga
     3301 ccagccgggc cccgcgcgcg cgcagccatt gcacctcctc gtcgatccgc cgctcgttgc
     3361 gcgcgatcag ttcctcggcc cgctccagcg tccgccgcag gtcgatccgc gtcgcgtcgg
     3421 gtcccagcgt gccgcaatcg aaggcggccg gcgccagctc gaacggccgt cccatcagct
     3481 tttgttccag aaaccagcgc gggcactccg tccgcagcgt caccgggatt tcgcgatcca
     3541 cccgcgccag gatcagcgtc gagcgcaccg cgtgcccaaa tccatgcgag gtgatatacc
     3601 agacgatcat ggcccggccc cgtcggcgac gcagtgggcc tattgttcag gccgccgctg
     3661 cgcgcgcaag ccggaactgc tgctccgggt cgatcgcgct ctgcagccgg cggctcagcc
     3721 ggcgctcctc ggccttcagc aggcgcaaca ccccgctcgg cattacccca ttcccccgtc
     3781 ggacttgcgc cgctcggcca tccccactaa aattaaccct ctcccactcc cacaggctgc
     3841 aaggagcatc ggctgtatgg ctctcaccta ctccaccatg atggaacttg gcaccacggc
     3901 ccccgatttc agcctgccgg ggaccgatgg ccagctctgg cgccgcgacg acgccaaagg
     3961 cacccgcggc acgctcatcg tcttcacctg caaccattgt ccctacgcca aggccgtgga
     4021 agatcgcctg atcgccatcg gccgcgacta tcagccgcgc ggcatcggca tcgtcctgat
     4081 caacagcaac gacgccgaca attaccccga ggattcgttc gacaagatga aggagcgcgc
     4141 cgccgccaga aactatccct tcccctacct gttcgacgaa agccaggacg tggcgcgcgc
     4201 ctaccacgcg gcctgcacgc ccgacccctt tctgttcgac cgccatctgc gcctctacta
     4261 ccgtggccgc atcgacgaca actggcagga tccggacaag gtccgccgcc acgatctgcg
     4321 cgacgcgctt gacctgctgc tcgccgacaa gcccgccccc gtcgagcaac tcccctccat
     4381 gggctgcaac atcaaatgga aggaatgagg cgggccggct cacggcgcgt ggcgtccccg
     4441 gccgccgaga tatccctgat gacatttcca cccgtccgct acgtgcaggc cgttcccgtc
     4501 atgcacgacg gccagcatgt ggttgtgttg caggatcccg agggcctgtg tcccgagacg
     4561 ctcgccgttc ccctgccgct ctacctgatc atgacgctcc tcgacggccg gcgcgacgcc
     4621 cgcgccgtcc agaccgaaat cgcccggatg gcgcaggggc agatcgtccc cacccaggag
     4681 atcgagcgca tcgttcacga gctggatgaa ctctatctgc tggagaacgc ccgcgcgcgc
     4741 gaacgccgcg ccgaactgga gcgcgaattc gacgcgctcc cgctgcgccc gccgatccac
     4801 gccggcgccg cctaccccgc cgaggccgac gcgctccagg agatgatgcg gcaactgttc
     4861 gacggcctga ctccggccgc cgccgacgcc ccccgcccgc gcggcctcat cctgccgcac
     4921 atcgacctgc gcgtcggcgg ccgcgcgctg gcccgcgccc tcgccacgct cgatgccgac
     4981 cg
//
LOCUS       DAOPCO010000298         4957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14297_length_4957_cov_5.362097, whole genome shotgun sequence.
ACCESSION   DAOPCO010000298 DAOPCO010000000
VERSION     DAOPCO010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14297_length_4957_cov_5.362097"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="PLS90_15095"
     CDS             complement(<1..1187)
                     /locus_tag="PLS90_15095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPK03772.1"
                     /translation="MTDRSPPLALTDLAGEWAGLAPAPLDARLRELRGQGRAILDLVT
                     ANPHEQGLTFPGELLAEISAEAMQSIGVYRPEPRGLAAARKAVAQWYARRGSPAAADA
                     IQLCPGTSTGYGWALRLLAEAGDEILAPSPGYPLFDDLAAVAGLRLRKYYLRFDGGGW
                     RHAVDEIDYQCTARTRAVVLISPHNPTGAMATAEELAALAEVCRRRRLALLFDEVFCE
                     FREDRAHPAPRPHGEQFPLAITLNGCSKMLSLPGWKLAWMKVDGEPRHVRPFMTALEH
                     LADTYLPLPDLQQAMLPALLTRGEAVIDALAGAYQQRRRTARTALAFATDDCTAGVYL
                     CGRLSGAYAGPGGDDQFALDALEREGVLVHAGYFYDLPGRFVLPCIAAPATLRTGIDK
                     LNRL"
     gene            complement(1550..1942)
                     /locus_tag="PLS90_15100"
     CDS             complement(1550..1942)
                     /locus_tag="PLS90_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03773.1"
                     /translation="MKKNFLLRWPPVAAACVGLVCLTGCISLRIGTDNEPPDFKIDGI
                     GSAYATVTDIPAYDGTVVRFDLFRGGANTGELASFYIWPLGGLDLGLLGARLRLLPLE
                     MGAGTLFYQPGPVTGDASPGVKATIDAE"
     gene            2169..3287
                     /gene="rodA"
                     /locus_tag="PLS90_15105"
     CDS             2169..3287
                     /gene="rodA"
                     /locus_tag="PLS90_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPK03774.1"
                     /translation="MAKARFRLVPYRLLPWIDWLLIALILLLLAAGLITMWGATSSGD
                     GPGPLQGYALRQLRWMGLGLTGMFVLLFFDYRWLRRVVWVLYGLAVALLGAVLVAGET
                     VNNARSWFNLGGFSLQPAEPAKIVVILSLGHYLARRGRVFRGLRHTFIPLALALVPML
                     LILKQPDLGTAAVFVPVVAAMFWAAGIRKWVVGLFLLAGIAVAAAGYAHLKPYQKDRI
                     LTFLNPEADPRGKGYNVLQAQTALGSGRLLGKGWGRGTQTHFRFLPEYHTDFIFPTVG
                     EQFGLAGTVAVLLLLTLFIWRMLDLARRVHDLFGVLIITGFAATLATHMILNIGMTIG
                     LLPVTGLPLPFFSYGGSFMLATLSMVGLTLSVAARRGL"
     gene            complement(3367..4497)
                     /gene="mqnC"
                     /locus_tag="PLS90_15110"
     CDS             complement(3367..4497)
                     /gene="mqnC"
                     /locus_tag="PLS90_15110"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HPK03775.1"
                     /translation="MFRMLEGSVHDPAIYPILERVVAGERLSDADAVRLFQSHDLLAI
                     GRAAQAVRWRLHPEPVVTYVIDRNINYTNICITDCSFCAFYRKAGDADAYVRTREELR
                     QKIEETFEAGGSQILMQGGLNPELDLAWYEDLLRWMKQEFPGLHIHAFSPTEISFFRD
                     HFGLRLEEVLRRLRAAGLDSIPGGGAEILSDRVRRKISPDKTSADDWLEVMRVAHRLG
                     MRTSATMMFGIIETMEERVEHLRRLRELQDETGGFTAFIDWTFQPKNTPYKDKFAGAF
                     DYLRTTAIARLYLDNFENIQASWVTQGDKIAQLSLTMGVNDLGSLMLEENVVAAAGTN
                     FMYSRRELARWAADLGFELRQRDYYYRDYVHPAAQSAATAGI"
     gene            4731..>4957
                     /locus_tag="PLS90_15115"
     CDS             4731..>4957
                     /locus_tag="PLS90_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03776.1"
                     /translation="MTPPKTAAADRAEDGRHARIGGIDLTLAHPDVIEMKWIGNQRLR
                     DQLVAAWMTVAPSDIPLNPRLIGKPGVGKTTL"
BASE COUNT          779 a         1804 c         1505 g          869 t
ORIGIN      
        1 agccggttca gcttgtcgat gcctgtccgc agcgttgcgg gcgcggcgat gcagggcagc
       61 acgaaccgcc ccggcagatc gtaaaagtag cccgcatgca ccagcactcc ctcccgctcc
      121 agggcgtcca gcgcaaattg atcgtcgccg cccggcccgg cgtaggcgcc ggaaagccgg
      181 ccgcacaggt agacgcccgc cgtgcagtcg tccgtcgcaa atgccagggc cgtccgcgcc
      241 gtccgccgtc gctgctgata cgcccccgcc agcgcgtcga tgaccgcctc cccccgcgtc
      301 agcagggccg gcagcatcgc ctgctgcaag tcgggcagcg gcaggtaggt atcggccaga
      361 tgctccagcg ccgtcatgaa cggccgcacg tggcgcggct caccgtccac tttcatccac
      421 gccagtttcc agcccggcag cgagagcatc ttggagcagc cgttgagcgt gatcgcgagc
      481 gggaattgtt ccccgtgcgg gcgaggggcg gggtgcgcgc ggtcctcgcg aaactcgcag
      541 aaaacttcat cgaacagcag ggccagtcgc cggcggcggc agacctccgc cagcgccgcc
      601 agttcctccg ccgtcgccat cgcccccgtc gggttgtgcg gcgagatgag cacgaccgcc
      661 cgcgtccgcg ccgtgcattg atagtcgatt tcatcgaccg catggcgcca gccgccgccg
      721 tcgaaccgca gatagtattt gcgcaggcgc agccccgcga ccgccgccag gtcgtcaaac
      781 agcggatagc cgggcgacgg cgccaggatc tcgtcgccgg cttcggccag caggcgcaac
      841 gcccagccgt agccggtgct ggtgccgggg cagagctgaa tcgcatcggc cgcggcgggc
      901 gatccgcgcc gcgcgtacca ctgcgccacc gccttgcgcg ccgccgccag cccgcgcggc
      961 tccggccggt agactccgat ggattgcatc gcctcggccg agatctcggc caggagctcg
     1021 ccgggaaacg tcaagccctg ctcgtgcgga ttcgccgtga ccagatccag gatggcgcgc
     1081 ccctggccgc gcagctcgcg cagccgcgca tcgagcggcg cgggcgcaag cccggcccat
     1141 tcgccggcga ggtcggtcaa cgccagggga ggggagcgat cggtcatcga cgaacacagc
     1201 gataggatca ggcggcggac acgcgcgccg atccattagg ccccgtccgt tccggaatcg
     1261 ctattggtat cgcaatcgaa atcggcatcg ccatcgatcc ggaaacccga gagactgctc
     1321 cccccgtcga ggcagctttt tttggagtgc ggtgccagag cgttgcagcg caacgcgtcg
     1381 acaccgcttt ggattcgacc tgagccgagg ctgatcggtc caatgcggcg tcgccacgcg
     1441 ctgcgtgctt tctgccgccg cactccaaac gggcaacctg cccgacattc gactattgaa
     1501 tccttgaatc tgcctcgaac ttcgaacttc ggatttcgga tttcttcccc tactccgcgt
     1561 cgatggtcgc cttcaccccg ggtgaagcgt cgcccgtcac cggcccgggc tgatagaaca
     1621 gcgtcccggc gcccatttcc agcggcagca ggcgcagccg cgctcccagc aagcccagat
     1681 ccagcccgcc gagcggccag atgtaaaatg aggccagttc gccggtgttc gccccgccgc
     1741 ggaacaggtc aaaccgcacg accgtcccgt cataggccgg gatgtcggtc accgtcgcat
     1801 aggcgctccc gatgccgtcg atcttgaaat cggggggttc gttgtcggtg ccgatccgca
     1861 ggctgatgca gccggtcaga cagaccagcc cgacgcacgc cgccgccacc ggcggccagc
     1921 gaagcaggaa atttttcttc atcgccgtat tcctctctca aaaatcgtct ctcgtcctcg
     1981 tgatcgcccc taaaggacgt ccctcgttcc tccccgaaaa tcatcctgca agcgattcac
     2041 gtgccagcga cgctgaagcc cgacccggat ttgtgttagg gtagcgggcg gcgtcgtagc
     2101 ccggaccacg ccggcgagct gcagcggtcc cggcgcgaac ggcccagccc ttcccctgag
     2161 cgactgacat ggccaaggcc cgattccgtc tggttcccta tcgtctgctg ccttggatcg
     2221 attggctgct gatcgcgctg atcctgctgt tgctcgcggc cggcctgatc acgatgtggg
     2281 gcgccacctc gtccggcgac ggaccgggtc ccctgcaagg ctacgccctg cgccagttgc
     2341 gctggatggg attgggcctg accggcatgt tcgtgctgtt gttcttcgac tatcgctggt
     2401 tgcggcgcgt cgtctgggtg ctctacggtc tcgccgtggc gctcctcggc gccgtcctcg
     2461 ttgccggcga gaccgtcaac aacgcccgct cctggttcaa tctgggcggc ttctcgctac
     2521 agcccgccga accggccaag atcgtcgtca tcctgagcct gggccattat ctcgcccgcc
     2581 gcgggcgcgt gttccgcggc ctgcgccata cgttcatccc gctggcgctg gccctcgtcc
     2641 cgatgctgct gattctgaag caacccgatc tgggcacggc ggccgtcttc gtcccggtcg
     2701 tcgccgcgat gttctgggcc gccggcatcc gcaaatgggt cgtcggcctg tttctgctgg
     2761 ccggcatcgc cgtcgcggcg gccggctacg ctcacctcaa gccctaccag aaggaccgca
     2821 ttctcacctt tctgaatccc gaggccgacc cccgcggcaa gggctacaac gtcctgcagg
     2881 cccagaccgc gctgggatcg ggccggctgc tgggcaaggg ctggggtcgc ggcactcaga
     2941 cccatttccg cttcctcccc gaataccaca ccgacttcat cttcccgacg gtcggcgaac
     3001 agttcggcct tgccggcacc gtcgccgtcc tgctcctgct gacgctcttc atctggcgca
     3061 tgctcgatct ggcgcgccgc gtccacgatc tgttcggcgt cctgatcatc accggcttcg
     3121 ccgcgaccct cgccacccac atgattctca acatcggcat gaccatcggc ctgctccccg
     3181 tgacggggct ccccctgccg ttcttttcct atggcggctc gttcatgctg gccacgctca
     3241 gcatggtcgg cctgacgctg agcgtcgccg cccgccgcgg actttaagtc tccgatccac
     3301 gggtcagtcg tccgtgcgcg cccgtctctg tgtgtgcggg ttcgtgcgtc ttggccggtc
     3361 gtgcggtcag atgccggcgg tcgcggcgga ttgcgccgcc ggatggacgt aatcccggta
     3421 gtaataatcg cgctggcgca gttcgaatcc cagatcggcg gcccagcgcg ccagctcccg
     3481 ccggctgtac atgaaattcg tccccgccgc cgccaccaca ttctcctcca gcatcaggct
     3541 gcccagatcg ttcaccccca tcgtcagcga gagctgcgcg atcttgtccc cctgcgtcac
     3601 ccaactggcc tggatgttct cgaaattgtc cagatacagc cgcgcgatcg ccgtcgtccg
     3661 cagatagtcg aacgccccgg cgaacttgtc cttgtacggc gtgttcttcg gctgaaacgt
     3721 ccagtcgatg aacgcggtga agccccccgt ttcatcctgc agctcgcgca gccgccgcag
     3781 gtgctccacc cgttcctcca tcgtctcgat gatgccgaac atcatcgtcg cgctggtgcg
     3841 catccccagc cggtgcgcga cgcgcatcac ttccagccag tcgtcggccg aggtcttgtc
     3901 gggcgagatc ttccgccgca cgcgatcgct caggatttcg gccccgcccc cggggatcga
     3961 atccagtccc gccgcccgca gccgccgcag cacctcctcg agccgaagcc cgaaatggtc
     4021 gcggaaaaac gagatctccg tcggcgagaa cgcgtggata tgcagccccg gaaactcctg
     4081 cttcatccag cgcagcagat cctcgtacca ggccaggtcc agctcggggt tgagcccccc
     4141 ctgcatcagg atctggctcc cccccgcctc gaatgtctcc tcgatcttct ggcgcagttc
     4201 ctcccgcgtg cgcacgtagg cgtccgcgtc ccccgctttg cggtagaacg cgcagaagga
     4261 acaatccgtg atgcagatat tcgtgtagtt gatgttgcgg tcgatgacgt aggtgacgac
     4321 cggctcggga tgcagccgcc agcgtacggc ctgcgcggcc cgcccgatcg ccagcaggtc
     4381 gtgcgactgg aacagccgga cggcgtcggc gtcgctcagc cgttcgcccg cgacgacgcg
     4441 ctccaggatc gggtagatcg ccgggtcgtg tacggagcct tcgagcatgc gaaacatcca
     4501 gcctttcctc agccgtgcgc gcggaaccca gtgtagcgac tttcccaccg ccgaaccaag
     4561 ccgaaaccac gcccgacccg caccgcccga gcgcgggaag cacgattctc gaatttcgaa
     4621 atccgaaaca aatacgcgct caaaccaggg gcaccttcgg cgttgcggcc ggaccgcccc
     4681 cacgagtaag ctggttgttt gacatcgttc acgggagttc cgcgctatcc atgacgccgc
     4741 ccaaaaccgc cgccgctgat cgagccgagg acgggcgcca tgcccgcatc gggggcattg
     4801 acctgaccct cgcccatccc gacgtgatcg agatgaagtg gattggcaac cagcgcctgc
     4861 gcgaccagtt ggtggccgcc tggatgaccg tcgccccgtc cgatatcccg ctcaatcccc
     4921 gcctgatcgg caagcccggc gtcggcaaga cgaccct
//
LOCUS       DAOPCO010000299         4930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14379_length_4930_cov_4.926359, whole genome shotgun sequence.
ACCESSION   DAOPCO010000299 DAOPCO010000000
VERSION     DAOPCO010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14379_length_4930_cov_4.926359"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PLS90_15120"
     CDS             complement(<1..137)
                     /locus_tag="PLS90_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPK03777.1"
                     /translation="MPPAFPQLAGRRIIVAVTGGIAAYKVADCVSRLRKTGAEVRVAM
                     T"
     gene            379..903
                     /locus_tag="PLS90_15125"
     CDS             379..903
                     /locus_tag="PLS90_15125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPK03778.1"
                     /translation="MRDRPRNIILTGFMGTGKSSIGRRLARRLAWRFVDTDNLIEERA
                     GQPIAAIFAEAGEAAFRAIEREVAHDVGSLRRHVVATGGGMVVDPENLELLEQAGLVI
                     LLEATVETIWERTRGRKHRPLLNNDDPRGTIERLLAARRAAYERVAHRLATDGRTPDE
                     VVAEILKLYEGAAA"
     gene            900..2012
                     /gene="aroB"
                     /locus_tag="PLS90_15130"
     CDS             900..2012
                     /gene="aroB"
                     /locus_tag="PLS90_15130"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPK03779.1"
                     /translation="MNPIEKLPTQVDVALDERSYPIVIDADWLDRIGAALAPYFPPRP
                     CTLVTNPTVGRHHAEGVRRALAEAGYRVSICEIPDGEEHKNVASLTRIWDHMLRERHT
                     RESGIVALGGGVVGDIAGFAAATLLRGIAFIQLPTTLLAMVDSSVGGKTGINHEAGKN
                     LIGAFWQPVFVGIDLRMLETLPDEELRSGMAEVIKHGIIADRAMFEFLEQHIERALDR
                     DPHVLAYLVRRSCEIKADVVARDEREEGLRAILNFGHTFGHAAEALSNYNQIRHGEGV
                     AMGMVAAMRLAARRGVVGDEEARRVEQLCRRAGLPTRLLPFSAEQYWRLMGSDKKVRD
                     GRVRFVLPTRLGHVEVYADVGRDEVEACLRECGAAG"
     gene            complement(2314..2760)
                     /locus_tag="PLS90_15135"
     CDS             complement(2314..2760)
                     /locus_tag="PLS90_15135"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPK03780.1"
                     /translation="MKTVRNLLDHKGRHVSSISAADTALEAAHRMNDQRIGSLVVTAD
                     GKVIGIITERDILTRIVAVERDPSATRVEEIMSHPVACCRLDTTVEECRSVMTVKRIR
                     HLPVVEEGRLMGIITSGDILASEIQEHKTTIEYLNEYLYSSGAPHG"
     gene            complement(2905..3387)
                     /gene="tsaE"
                     /locus_tag="PLS90_15140"
     CDS             complement(2905..3387)
                     /gene="tsaE"
                     /locus_tag="PLS90_15140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPK03781.1"
                     /translation="MEPRSASAGAGAGHGEPLPAPPWSFVSSGIEETLALGRRIGAAC
                     QGGEVILLDGPMGAGKTLLTQGIAAGLGIDGAIVSPTFVVMRTYRGGRGLTLHHFDFY
                     RLGGEEDLESVGLEDCLRGDAVVVIEWPSRAPAAAGRHTLLLRLAVVGEEARRISAEG
                     "
     gene            complement(3387..>4930)
                     /locus_tag="PLS90_15145"
     CDS             complement(3387..>4930)
                     /locus_tag="PLS90_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK03782.1"
                     /translation="GSGGGGAGADAPGEPVTVRINQGSDVLSMDPYLQHESPTMCVLR
                     NVFDTLTDYDRRIELQPCLAESWTNDGDRVWTFKLKEGVTFHGGQPFTAEDAAFSLDR
                     ALNWEKSRTRSEIQTIEAVEAIDPLTLRITTRLPDAILPLRLASVLIMDQESTEQGLA
                     EHGEAWLASHANGTGAYRVAEWIKDAQCVLERFDGYWGEPPVVERLVFIPTSQDATRM
                     VNLQRGGVDLVVNVPPTQVKQVEALPGYRTIKSPSLRLIYLGLDVGRDESPRIPGSPP
                     NPLKDARVRQAIMKSIDNKAIVESIMGGNAEPIDQLLPPGVTGHDPSIQLERPDYEEA
                     RRLLAEAGYPDGFTVRLDGPNDRYVNDARIISAVAQELARCGIQVEPNAVPKARFFSE
                     EQQGNCSFFLIGWSNTNGDGQGTFDHLLHTPDPENNLGVANASTNYSNPRLDEICRQA
                     TVEFDPARREALLREANRLAIADLPHIPLHLQMDIYAIADRLDWTPRRDTQVRGVDIR
                     LAAAP"
BASE COUNT          784 a         1685 c         1618 g          843 t
ORIGIN      
        1 tcggtcatcg ccacgcgcac ctcggccccc gttttgcgca gccggctgac gcaatcggcg
       61 accttatagg ccgcgatgcc gccggtcacc gccacgatga tccggcgacc tgcgagttgg
      121 gggaaggctg gcggcatggt tggctgggcg ggctttcgag gcattccatc tcgacgcact
      181 ggacgagtta tgccataaac aagcgcgacg agacaacctc tcgtgtacga tgggttgcgt
      241 tagccgaggc gagggatcag atcacgaacg acacgaatgg gcacgaagtt tttttgtccc
      301 ttttgtccct gatgtccttt ctgtctctgc ttcccctgga caaccgatga cgatggtatt
      361 cataaattgg aaagcaatgt gcgcgaccga ccgcggaaca tcatcctgac cgggttcatg
      421 gggacgggca agtcgagcat cgggcggcgc ctggcgcggc ggttggcgtg gcgcttcgtc
      481 gataccgata acctgatcga ggaacgggcg ggtcagccga tcgcggcgat tttcgccgag
      541 gccggcgaag cggcgtttcg cgcgatcgaa cgcgaggtgg cgcacgatgt cggatcgctg
      601 cggcgccacg tcgtcgccac cggcggcggg atggtcgtcg atccggaaaa cctcgagttg
      661 ctcgaacagg ccgggctggt gatcctgctg gaggcgacgg tcgagaccat ctgggagcgg
      721 acgcgcgggc gaaaacaccg gccgctgctg aacaacgacg atccgcgcgg gacgatcgaa
      781 cggctgttgg cggcccggcg cgcggcctac gagcgcgtcg cgcaccggct ggccaccgac
      841 ggccgtacgc ccgatgaggt cgtcgccgaa attctgaagc tgtatgaagg agcggcggcg
      901 tgaacccgat cgaaaaactt ccgacgcagg tggatgtggc cctcgacgag cgttcgtacc
      961 ccatcgtgat cgacgcggac tggctggacc ggattggcgc cgcgctcgcc ccgtatttcc
     1021 ctccccggcc atgcacgctg gtgaccaacc cgaccgtggg gcgtcaccat gccgaagggg
     1081 tgcggcgcgc gctggccgag gcaggctatc gcgtttcgat ctgcgaaatt cccgacggcg
     1141 aggaacataa gaatgtggcc tcgctgaccc gcatctggga tcacatgctg cgcgagcggc
     1201 acacgcgcga gagcgggatc gtcgcgctgg ggggcggcgt ggtgggcgac atcgccggct
     1261 tcgccgccgc gacgctgctg cgcgggattg ctttcattca gctcccgacg acgctgctgg
     1321 cgatggtcga cagcagcgtg gggggcaaga ccgggatcaa ccatgaggcg gggaagaacc
     1381 tgatcggcgc cttctggcag ccggtcttcg tcggcatcga cctgcgcatg ctcgagacgc
     1441 tgccggacga ggaactgcgt tccgggatgg ccgaggtgat caagcacggg atcattgcgg
     1501 accgcgcgat gttcgagttc cttgagcagc acatcgaacg ggcgctcgat cgcgacccgc
     1561 atgtgctggc ctacctggtt cggcgctcgt gcgagatcaa ggccgatgtg gtggcgcgcg
     1621 acgagcgcga ggagggcctg cgcgcgattc tgaacttcgg ccacacgttc ggccacgccg
     1681 ccgaggcgct ttccaattac aaccagatcc gccacggcga aggggtcgcg atggggatgg
     1741 tggccgcgat gcggctggcg gcgcgccggg gagtcgtcgg cgatgaggag gcgcgccggg
     1801 tcgaacagct ctgccggcgg gccgggctgc ccacccggct gctgccgttc agcgccgagc
     1861 agtactggcg cctcatgggc tcggataaga aggtgcgcga cggccgcgtg cgcttcgtgc
     1921 tgccgacccg gctgggtcac gtcgaggtct atgccgacgt cgggcgcgac gaggtggaag
     1981 cctgcctgcg ggagtgcggc gccgcaggct gagccgctct cgtccccgcc ctcggctctc
     2041 gataagctcc actcttttcc ctcgttttaa gcccgtgtca ttcccccaat cgcaagctgc
     2101 tgaaagattc cagtctcact gcaggcggct cctgctgatc cgttcagtcc gcgaagcgga
     2161 cgactggaat caagcgcagg gtggcggccg caggccggaa ccctgcgtcg ccggcctcga
     2221 acgaaacccc tggaaggggt ggccggagcg ggtttgaatt tggggaatga caagatttca
     2281 agccgtgcgg tccgttcagc ccgcggggtc cgctcagccg tgcggcgcgc cggacgaata
     2341 cagatactcg ttcaaatatt caatcgtcgt cttgtgctcc tggatctcgc tggccaggat
     2401 gtcaccgctg gtgatgatcc ccatcagccg cccctcctcc acgaccggca ggtggcggat
     2461 gcgcttgacc gtcatcaccg accggcattc ctcgaccgtc gtatcgagcc ggcagcaggc
     2521 caccgggtgc gacatgatct cctcaacgcg cgtcgccgac ggatcgcgtt ccacggcaac
     2581 aatccgggtc aatatgtcgc gctcggtgat gattccgatc accttgccgt cggcggtcac
     2641 caccagcgac ccgatccgct ggtcgttcat ccggtgggcg gcttccaggg ccgtatcagc
     2701 ggcgctgatg gaagagacgt ggcgtccctt gtgatcgagc agattcctga cggtcttcat
     2761 cggattgtcc tttcgtccgc gcgagggacc tccggcgccg cggaatgggc ttttattacc
     2821 atgagatcat gaaggaattg tcccgccgcc gccgccgccg cgcaagcaca aagccacccg
     2881 ccggccgcct tcccgcgcgc cgtctcaccc ctccgcgctg atccggcgcg cctcctcgcc
     2941 cacgaccgcc aggcgcagca gcagcgtgtg gcgcccggcc gccgccggcg cgcggctcgg
     3001 ccattcgatc accaccacgg cgtccccgcg cagacagtcc tccagaccga cgctctccag
     3061 atcttcctcg ccgcccagcc ggtaaaaatc gaagtggtgg agcgtcagcc cgcgcccccc
     3121 gcggtacgtg cgcatcacca cgaacgtcgg gctgacgatg gccccgtcga tccccagccc
     3181 cgccgcgatc ccctgcgtca gcagcgtctt gccggccccc atcgggccgt cgagcaggat
     3241 gacttcgcct ccctggcacg ccgcgccgat ccgccgcccc agcgccagcg tttcctcgat
     3301 gccgctcgaa acaaacgacc acggcggcgc gggcagcggc tcgccgtgcc ccgcgccggc
     3361 gccggcgctg gccgaccgag gctccatcac ggcgccgccg ccagccggat gtcgacgccg
     3421 cggacctgcg tgtcgcgccg cggcgtccaa tccagccgat ccgcgatggc gtagatgtcc
     3481 atctgcagat gcagcgggat gtgcggcagg tccgctatcg ccagtcggtt ggcttcccgc
     3541 agcagcgcct cgcgccgggc cgggtcgaat tcaaccgtgg cctggcggca gatctcatcc
     3601 agccgcggat tcgagtaatt ggtcgatgca ttggccaccc ccagattgtt ctccgggtcg
     3661 ggcgtgtgca gcagatgatc gaacgtcccc tgcccgtcgc cattggtgtt ggaccagccg
     3721 atcaggaaaa acgagcagtt gccctgctgc tcctcgctga agaaccgcgc cttggggacg
     3781 gcgttgggct cgacctgaat cccgcaacgc gccagttcct gcgccaccgc cgagatgatc
     3841 cgcgcgtcgt tcacgtagcg gtcgttcggt ccatcgagcc gcaccgtgaa gccgtcggga
     3901 tagcccgcct cagccagcag ccgccgcgcc tcctcatagt ccggccgctc cagttggatc
     3961 gaggggtcgt gccccgtcac gcccggcggc agcagctggt cgatcggttc ggcgttgccg
     4021 cccatgatcg actcgacgat cgccttgttg tcgatgctct tcatgatcgc ctggcgcacg
     4081 cgcgcgtcct tgagcggatt gggtggcgat ccggggatcc tcggcgactc gtcccggccc
     4141 acgtccaggc cgaggtagat cagccgcagg ctgggcgact tgatcgtgcg atagcccggc
     4201 agcgcctcca cctgcttgac ctgcgtcggc ggcacgttga ccaccaggtc cacgcccccc
     4261 cgctgcagat tgaccatccg cgtcgcatcc tgtgaggtgg ggatgaacac cagccgttca
     4321 acaaccggcg gctcgcccca gtaaccgtcg aaccgctcca gaacgcactg cgcatccttg
     4381 atccattccg ccacgcgata ggccccggtg ccgttggcgt gcgaggccag ccacgcttcg
     4441 ccgtgctccg ccagcccctg ctcggtcgat tcctggtcca tgatcagcac cgaggccagc
     4501 cgcagcggca gaatcgcgtc gggcagccgc gtcgtgatgc gcagcgtcaa cgggtcgatt
     4561 gcctcaaccg cttcgatggt ctggatctcc gagcgcgtgc gcgatttctc ccagttcagc
     4621 gcccggtcga gcgaaaacgc cgcgtcctcg gccgtgaacg gctggccgcc gtgaaacgtc
     4681 accccttcct tcaacttgaa tgtccacacg cggtcgccgt cattcgtcca gctctcggca
     4741 aggcacggct gcaactcgat ccgccggtca tagtcggtca gcgtgtcgaa gacgttgcgc
     4801 agcacgcaca ttgtcggcga ctcgtgctgg agatacgggt ccatcgagag cacgtccgag
     4861 ccctggttga tccggacggt gaccggttcg ccgggcgcat cggccccggc gccgccgccg
     4921 cccgatccgc
//
LOCUS       DAOPCO010000300         4902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14472_length_4902_cov_5.450382, whole genome shotgun sequence.
ACCESSION   DAOPCO010000300 DAOPCO010000000
VERSION     DAOPCO010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_4902_cov_5.450382"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2147
                     /locus_tag="PLS90_15150"
     CDS             <1..2147
                     /locus_tag="PLS90_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPK03783.1"
                     /translation="AFQVGRGRARVLARAPEAEADESFTFSAGLACNRCGRAYPTPEP
                     QLFSFYSPLGACPACEGFGRVIELDLDKILPNRNLCIEDGAIAPWNSEGNLEMYDMLR
                     AHTTPKQIPRLQPLSTFTDAQWRNLLDGCGEFPGIRGYFKWLESKRYKVQARVMLARY
                     RAYVRCEACAGARLRPEALAFTFQGLNIAELNRLPVREVLALFEHLTLPPAEMEVAGR
                     ALETLLARLRYLEGIGLGYLTLDRQTRTLSGGESQRINLATALGSALTDTLYVLDEPT
                     VGLHPRDTHRLIGILHTLRGLGNTVVVVEHDLDVIRSADRLIDMGPGAGERGGEILYE
                     GPIAELNGQDTPTARHIRQYAEPSVPPLGRAPKGWITVRGARGHNLRNLTVRFPLGVF
                     ACVTGVSGSGKTTLVRDTLCAHYRRLREIAPVEAEPCDRIAGLDQIDSLQWVDQTPLA
                     GSKRSNPVTYAKAYEWIRKTLAATREARALGVTARDLSFNVDGGRCTVCHGTGRQIID
                     MHFMADVEVVCEACDGRRFQERVLRLEWNGLNIHQILELTVDEATKFFREAPKIVRAL
                     KPLRDVGLGYLRLGQSTITLSGGEAQRLKLAAHLATMRDGERRMFVFDEPTTGLHPED
                     LQRLIEIFQQMVKRGCTLLVVEHNLDLIAAADWIIDLGPEGGDGGGRLVAEGTVEQIM
                     ACEESLTGQYLRRRFETTTRAATERDPSANDA"
     gene            complement(2223..>4902)
                     /locus_tag="PLS90_15155"
     CDS             complement(2223..>4902)
                     /locus_tag="PLS90_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03784.1"
                     /translation="TMTLRPLDGIGQKEELIAELMTRAPNNYGPVENQVQVYLNSLKG
                     GSVTAEQAEGLGRAIWAERAARDGAEYAEQLIEKTLKGLATVIGDVIKEFNKFQRKGI
                     KERRKKLKQLNEKLDTETLRSGGFRLDGTTVEKTELINAIDSLLTEDPAFFLSVVARH
                     VKTAGKLAAQFIYGVLRGFGMEDSRAKQVADAFKLCVNIITEIVIHQGVGSSEAVVKL
                     IIEKALAQAKEPLFDGGSLNFPFSYTAFTKDQLEFARGRFESWSAANPEAYREDTRRA
                     ARIIQETNTGAANALGNAELALAFGGAFDTAESVFGTVGIVIPWSKFVAKTFKILRLI
                     SNSTAFVLPLAQVAYFVPEGVEAAVYRSFGQAPPAKTAAAAPSAPKAELGNVNERLET
                     EARAAAGLWRERLDELRAAWEENHLTSIIDVMTTTSVTNLPEAAKQFNRLALAYMIQF
                     SGAETPFFNNLALLPPVQESQFALHVEWTALNDELRSLLVDVMALSFAGADDPLYLAR
                     RDVLLAGIADLRQSIDRWIESLDILETSTGGYALLPTVYIEEVSVVAQATGGPTITQA
                     GEAVEIRAQVRNLGLDGAGSISLRAEIAVTTHPLVLTGDALALPPGGVLAGDDNQPGG
                     PDEVELAWQAQYTGDLTGIKRIPVQLRVLEGGAEPAGFNAIDELVILELDPMVIDSDV
                     DGLPDDYEQAHGLILGEDDAELDLDNDGLANESEFDLGTAPDGMDSDGDGLADGEETL
                     AGADGFVTDPLASDTDGDGMPDRADANPLDGDPASESQPLPEPSVEVDRAQVVLNAQS
                     PVAAIAVTNGGAGTLSWTAINDDGLLALGLAEGEVRGAGELLLIGVPAGQDLSGMTTL
                     AATIRVIDVGGVVPDEQLIQVRIGGEAGSGDWREYP"
BASE COUNT          772 a         1819 c         1482 g          829 t
ORIGIN      
        1 ccgcctttca ggtcggccgc ggccgggccc gcgtcctcgc gcgcgcgccc gaggccgaag
       61 ccgacgagtc cttcaccttc tcggcgggcc ttgcctgcaa ccgctgcggc cgcgcctacc
      121 cgacccccga accgcagctc ttctcgtttt acagcccgct cggcgcctgc cccgcctgcg
      181 agggcttcgg ccgagtcatc gagctcgacc tggacaagat cctccccaac cgcaacctgt
      241 gcatcgagga cggagcgatc gcgccgtgga acagcgaggg caacctcgag atgtacgaca
      301 tgctgcgcgc gcacaccacc cccaagcaga tcccgcgcct gcagccgctc tcgaccttca
      361 ccgacgcgca gtggcgcaac ctgctcgacg gctgcggcga gttccccggc atccgcggct
      421 atttcaaatg gctcgaaagc aaacgctaca aagtccaggc gcgcgtgatg ctcgcgcgct
      481 accgcgccta tgtccgctgc gaggcctgcg ccggcgcccg gctgcggccc gaggcgttgg
      541 ccttcacgtt tcagggcctg aacatcgccg agctgaaccg cctgccggtg cgtgaggtcc
      601 tcgccctgtt cgaacatctc acgctgccgc cggcggagat ggaagtcgcg ggccgcgcgc
      661 tcgaaaccct gctcgcacgc ctgcgctacc tcgaggggat cggcctgggc tacctgaccc
      721 tcgaccggca gacacgcacc ctgtcgggcg gcgaatcgca acggatcaac ctggcgacgg
      781 cgctggggtc ggccctcacc gacacgcttt acgtcctcga cgagccgacc gtcggcctcc
      841 acccgcgcga cacccaccgc ctgatcggca tcctgcacac gctgcggggc ctgggcaaca
      901 cggtggtcgt tgtcgagcac gatctggacg tgatccgttc ggccgaccgg ctgatcgaca
      961 tgggacccgg cgcgggcgaa cgcggcggcg agatcctcta cgaaggcccg atcgcggagc
     1021 tgaacgggca ggatacaccg acggcgcggc acatccggca gtacgcggag ccgtcagtgc
     1081 ctccgctggg acgcgctccg aaaggctgga tcaccgtccg cggggcgcgc gggcataatc
     1141 tgcgcaacct gaccgtgcgt ttcccgctgg gcgtcttcgc gtgcgtgacg ggcgtatcgg
     1201 gatccggcaa gacgacgctg gtgcgcgaca cgctgtgcgc ccattaccgg cgattgcgcg
     1261 agatcgcgcc ggtcgaggcc gagccgtgcg accgcatcgc cggcctcgat cagatcgact
     1321 cgctgcagtg ggtcgatcag accccgctgg ccggctccaa acgctccaac cccgtgacct
     1381 acgccaaagc ctatgagtgg atccgcaaaa ccctcgccgc gacccgcgag gcgcgcgctc
     1441 tcggcgtcac cgcccgcgac ctctcattca acgtcgacgg cgggcgctgc accgtctgcc
     1501 acggcaccgg tcgccagatc atcgacatgc atttcatggc cgacgtggag gtcgtctgcg
     1561 aggcctgcga cgggcgccgg ttccaggaac gcgtgctgcg cctggagtgg aacggcctca
     1621 acatccatca gatcctcgaa ctgaccgtcg atgaggcgac gaagtttttc cgcgaggcgc
     1681 cgaagatcgt ccgcgcgctc aaaccgctgc gcgatgtcgg cttgggctat ctccggctcg
     1741 gccagagcac cattacgctt tcgggcggcg aggcgcagcg cctcaagctg gccgcgcatc
     1801 tggccacgat gcgcgatggc gagcgccgca tgttcgtctt tgacgagccg accaccggcc
     1861 ttcatcccga ggacctgcaa cgcctcatcg agatctttca acagatggtc aagcgcggct
     1921 gcaccctgct cgtcgtcgaa cacaacctgg acctcatcgc ggcggccgac tggatcatcg
     1981 atctcggccc ggagggaggc gatggcggcg gccggctcgt cgccgaagga accgtcgagc
     2041 agatcatggc ctgcgaggaa tccctgaccg gtcagtacct gcgccggcga tttgaaacga
     2101 cgacgcgcgc cgcgaccgag cgcgatcctt ccgccaacga cgcctgatcc ccgccccgtc
     2161 actacctgcc ccgcccgcgc ttcggcgttt gccctcgagg tctccccgac ccccgtcacc
     2221 gcctacggat actctcgcca atccccggaa cccgcctcgc cgccgatccg gacctggatc
     2281 agctgttcat ccggcacgac gccgccaacg tcgatgaccc ggatcgtcgc ggccagcgtg
     2341 gtcatcccgg acagatcctg cccggcggga acgccgatca gcagcagctc gcccgcgcca
     2401 cggacttctc cctccgccaa tcccagcgca agcagcccgt cgtcgttgat cgccgtccac
     2461 gagagcgtcc ccgcgccgcc gttcgtgact gcaatcgcgg cgaccggcga ctgtgcgttg
     2521 agtaccacct gcgcacggtc gacttcgacg ctcggctcgg gcagcggttg cgactccgac
     2581 gccggatcgc catcgagcgg attggcatcc gctctatccg gcatcccatc accgtcggtg
     2641 tcgctcgcca gcggatcggt gacgaacccg tccgcgccgg cgagggtttc ctcaccgtcg
     2701 gccagcccgt cgccgtcgct gtccatgcca tcgggcgcgg ttcccagatc gaactcactc
     2761 tcattggcga gaccgtcgtt gtccagatcc agctccgcgt catcctcgcc caggatcagc
     2821 ccgtgcgcct gctcatagtc gtccggaagc ccgtcgacgt ccgaatcgat caccatcgga
     2881 tcaagttcaa ggatgacgag ttcgtcgatg gcattgaacc cggccggctc ggcgccgcct
     2941 tcgagcacgc gcaactgcac ggggatgcgc ttgattccgg tcagatcgcc tgtgtattgc
     3001 gcctgccagg ccagttcgac ctcgtcgggc ccgccgggct ggttgtcgtc gcccgcgagc
     3061 acgccgcccg gcggcagggc cagcgcgtcg cccgtcagca ccagcggatg cgtcgtgacg
     3121 gcgatttcgg cgcgcagcga gatcgacccc gccccgtcca gacccaggtt ccgcacctgg
     3181 gcgcggatct ccaccgcctc gcccgcctgc gtgatcgtcg gcccgcccgt cgcctgcgcc
     3241 acgaccgata cctcctcgat gtagaccgtc ggcagcagcg catatccgcc cgtcgacgtc
     3301 tccaggatgt ccaggctctc aatccagcgg tcgattgact ggcgcagatc ggcgatgccg
     3361 gccagcagca cgtcgcggcg cgccagatag agtgggtcgt ccgcgcccgc gaaactgagc
     3421 gccatgacgt ccaccagcag actccgcagc tcatcgttca gcgcggtcca ctccacatgc
     3481 agcgcgaact gcgactcctg caccggcggc agcagcgcca gattgttgaa gaacggggtt
     3541 tccgccccgc tgaactgaat catgtaggcc agcgcgagcc gattgaactg cttggccgcc
     3601 tccggcagat tggtcaccga cgtggtcgtc atcacatcaa tgatcgaggt caggtggttc
     3661 tcctcccagg cggctctcaa ttcgtccagc cgctcccgcc acaacccggc cgcggcgcgt
     3721 gcctcggtct ccagccgctc attgacgttc cccagctcgg ccttcggcgc cgacggcgcc
     3781 gcggcggccg tcttcgccgg cggagcctgc ccgaacgacc ggtagaccgc cgcctcgacc
     3841 ccctccggaa caaaatacgc cacctgcgcc agcggcagca cgaacgccgt gctgttgctg
     3901 atcagcctca ggatcttgaa tgtcttcgcc acgaacttcg accacgggat cacgatcccg
     3961 acggtgccga agaccgattc ggcggtgtcg aacgcgccgc cgaatgccag cgccagctcc
     4021 gcgttgccca gcgcgttggc cgccccggtg ttggtctcct ggatgatgcg ggccgcccgg
     4081 cgcgtgtcct cgcgataggc ctccgggttc gccgcgctcc agctctcgaa ccggccccgg
     4141 gcgaattcca gctggtcctt cgtgaaggcc gtatagctga acgggaaatt gagcgagccg
     4201 ccgtcgaaca gcggttcctt cgcctgcgcc agcgccttct cgatgatcaa cttgacgacc
     4261 gcttcggaac tcccaacgcc ttgatgaatt acgatttccg tgatgatgtt cacgcatagc
     4321 ttgaaggcgt cggccacctg cttggcgcgg ctatcctcca tgccgaagcc ccgcaagacg
     4381 ccgtagatga attgcgccgc cagcttgccc gccgtcttga catgcctggc caccaccgac
     4441 agaaagaacg cgggatcctc ggtcagcagg ctgtcgatcg cgttgatcaa ctcggttttt
     4501 tccaccgtcg tcccgtcgag ccggaatccc ccgctgcgca gcgtctcggt atcgagtttc
     4561 tcgttgagtt gcttgagttt cttgcgccgc tccttgatcc ccttgcgctg gaacttgttg
     4621 aactccttga tcacgtcgcc gatcaccgtc gccagcccct tgagcgtctt ctcgataagc
     4681 tgctcggcat attcggcgcc gtcgcgcgcc gcccgttcgg cccagatcgc gcggcccagc
     4741 ccctcggcct gctcggccgt caccgagccc cccttcaggg aattcaggta gacctgcacc
     4801 tggttctcga cgggcccgta attgttgggg gcgcgagtca tcagttccgc aatcaactcc
     4861 tccttctggc cgatcccgtc cagcggccgc agcgtcatcg tc
//
LOCUS       DAOPCO010000301         4860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14632_length_4860_cov_4.413736, whole genome shotgun sequence.
ACCESSION   DAOPCO010000301 DAOPCO010000000
VERSION     DAOPCO010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14632_length_4860_cov_4.413736"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PLS90_15160"
     CDS             complement(<1..1520)
                     /locus_tag="PLS90_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03785.1"
                     /translation="MRPRFCLPLWLGAVLLTAQPRAQVPNGGFETASPLQWTLTGDFT
                     LGTDGSATFGPAVTGNNYISSYKVDTPGAGIGTATSDEFQLDTPAVITYTGYTLGNGL
                     LFSLIRPQDNKQIARSRPTPLGSPVGLQALISYPYQDTDIQIVGNDNSGSDYLFADNF
                     QERPIRLLIDNFSPGGLSPSVWDLSTAGVWQSTSAVVGPWPDSRPAEGTHTAGTKYNG
                     QDNDGLGVLRTQPITIAAGDEAIGHYMSVATISPDFTNLTATLRNAADDAILAGPATI
                     QQGGGMWFWNEFDVSALAPGTQVYLEFTDNDAGGFMHIDFVCVLGSGAFESLVPGLQA
                     EPINLIPNGSVETASPLQWTLTDGFIRQTDATFGAATDGAAYLSTFFGGPPHDRVGTA
                     RSATFQLPAQPQIIRYQAYTQIGQIGWELRNGDGSELVPPVVKALPFGSPSSNAALVP
                     WPLTNAPIYVFGEDQDVEDYLYGDNFRTLPIRLLIDDFAQSRGLAATAWAGGAGDW"
     gene            complement(1872..>4860)
                     /locus_tag="PLS90_15165"
     CDS             complement(1872..>4860)
                     /locus_tag="PLS90_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPK03786.1"
                     /translation="SALRKLIYAPLSDQERGGICLRALLDSAGPVRAEAIRAMEQLGF
                     HRDTADAMQALFSGDPRAREAGLRRIGDLIGGLQPGERRIVLAVLVEALRESRPRDER
                     DPLLRLWLNLAPTLRQETDAAPEAARVMPIRIPAIASRLFLICLVAQLALIMPLSAEP
                     TFYVAATGSDAADGTIDQPFQSLARAQQAVRAANGAMTDDIVVYLRDGLHFLAEPLTL
                     TPLDSGGNGHWVRYEAYPGESPVISGGVLIDGWTLHDAARDIWRAPARGVNTRQLYVE
                     GRRAVRARSQGGLPGAVKTAAGYGTTDLGMQHWNNITDLEFVFEVEWRQPRCPVRAIA
                     GHLVSMQDPCWSHCNSWGAFSVDRPLYMENAYELLDEEGEWYLDRAEDVFYYKPLAGE
                     QLASARVIAPRLTALVRGAGQLNNKVHHILFKGLTFSHATWLGPNEDLGWANVQGTLR
                     KGDVAATMQMPGNLTFHAASNIAFSGNTFTRLGAVALEFLDGSQDNVINGNHFHDISG
                     SAIQIGFFTRHHASSPADYDRGNQITNNAIHDIGVEYQGCPAITNFYTDSTLIAHNEI
                     CHIPYSGISNGWGFGLVDSEADPSISNNNTFSHNHIHHYMQRMNDGAGIYSLGWQPGL
                     LIAHNYIHDAGTRLGDVKAGVYLDQGSRYAVVTQNVMHGQTFDLMVKGSDHQIRRNFS
                     TSQHRQGGRLTRVVPTAWGVETWDIRDNVWFEEPEIAPASIIRQAGLQRDFASLAHAP
                     AGDPTPPSPPDALTVTTVTEATIALAWSPASDDVAVTGYEIHADGVVAAATSALTATL
                     TGFAPATTYELFVRARDAAGNRSEPSETVYARTLPASTNLALQAPVWGFKPGGQHVAF
                     ANDHDVWRAVDGDPTSYARPTVDYLWDLKVDLRRVETIDRIVVDFPVAPRNDQTLHAT
                     RYRIEASLDGASWQVLREVNNSVGGRQTFGLDPIATRFVRLVAVRPNGAGQAGGVMAI
                     AELEIHRLPDPAPKTALKAWTMF"
BASE COUNT          863 a         1432 c         1705 g          860 t
ORIGIN      
        1 tgccagtcgc ccgcgccgcc ggcccacgcc gtggcggcaa ggccgcgcga ttgcgcgaaa
       61 tcgtcgatca gcaaccggat cggcagagtg cggaaattgt cgccatacag gtagtcctcg
      121 acgtcctgat cctcgccgaa cacatagatc ggcgcgttgg tcaacggcca ggggaccagc
      181 gcggcattgg aggagggcga accgaacggc agggccttga cgaccggcgg gaccagctcc
      241 gacccatcgc cgttgcgcag ttcccagcca atctggccga tctgggtgta ggcctgatag
      301 cggatgatct gcggctgcgc cggcagttgg aatgtcgccg agcgggcggt tcccacgcgg
      361 tcatggggcg gtcccccaaa gaacgtgctg agataggcgg cgccatcggt ggccgcaccg
      421 aacgtggcgt cggtctgacg gatgaagccg tcggtcagcg tccattgcag cgggctggcg
      481 gtttcgacac tgccgttggg gatcaggttg atcggctcgg cctgcaggcc gggcaccagt
      541 gactcaaatg cgccgctgcc caggacgcag acgaaatcga tgtgcatgaa tccgccggcg
      601 tcattgtcgg taaactcgag gtagacctgg gttccggggg caagcgccga aacatcgaat
      661 tcattccaga accacatccc gccgccttgt tgaatggtcg cggggccggc aaggattgca
      721 tcatccgcgg cgttgcgcag cgtggcggtc agattcgtaa aatcggggga aatggtcgcg
      781 acggacatat agtgcccgat tgcctcgtcg cccgccgcaa tcgtgatcgg ctgcgtgcgc
      841 aggaccccga gcccgtcgtt atcctgccca ttgtatttgg ttccggcggt gtgcgttcct
      901 tcggccgggc gcgaatcagg ccatggaccg accacggcac tcgtggactg ccagaccccg
      961 gccgtgctca gatcccaaac gctggggctc agcccgcccg gactgaaatt gtcgatcaac
     1021 aggcggatcg gtcgctcctg aaagttgtcg gcgaacagat agtccgagcc gctgttgtcg
     1081 ttgccgacaa tctgaatgtc ggtatcctgg tagggatacg agatcagggc ctggagtccg
     1141 acggggcttc caaggggggt tggcctggag cgcgcaatct gtttgttgtc ctgggggcga
     1201 atcaggctga agagcaagcc gttgccgagg gtgtagccgg tgtaggtaat gacggcgggg
     1261 gtgtcgagct gaaattcgtc ggaggtggcg gtgccgatgc ccgcgccagg ggtatcgacc
     1321 ttgtacgacg agatatagtt gttgccggtg acggccgggc cgaatgtggc ggagccgtct
     1381 gtgcccagcg tgaaatcgcc ggtcaacgtc cactgcaggg gtgaagccgt ctcaaaaccg
     1441 ccgttgggca cctgggcccg gggttgggcg gtcagcaaga cggcgccgag ccagagcggc
     1501 aggcaaaatc gagggcgcat cgtcgggttc tccttttggt ttgggattga agccatcgga
     1561 ctgcggggcg accgggtcga gggcgcttgt gggtgtgaaa gatcctgaag cgaacggatc
     1621 gcagtggaac ccgcgggaga tgaaggtcgg cgcgaggaag tggtcagacg gcgcccgggt
     1681 ccccgccgat ttcaacgatc atgcatgatc gtttatttag cgcggccgct cctcccccgt
     1741 caagcatctt tttcactctg ccgcggaatt cgtgcttcct tgagatagct cgtgctatag
     1801 caatcagacg gaaggggaca tgcgtgactg gaagactggg ctacgatctt cgcggagaac
     1861 cgacggcccg gctagaacat cgtccaggcc ttcagcgcgg tttttggcgc tgggtcgggc
     1921 aggcgatgga tctccaattc ggcgatggcc atgacgccgc cggcctgacc cgcgccattg
     1981 ggccggacgg cgaccaggcg gacgaaacgg gtggcaatcg gatccaaacc aaacgtttga
     2041 cgcccgccga ctgagttgtt cacctcgcgc agaacctgcc acgacgcgcc gtcgagggac
     2101 gcttcgatgc ggtagcgcgt ggcgtggagc gtctgatcgt tccgcggcgc gaccggaaaa
     2161 tcgacgacga tccggtcgat cgtctcgacg cgccgcagat cgaccttgag atcccaaagg
     2221 taatccaccg tgggcctggc ataggaggtt gggtcgccgt cgacagcccg ccagacatcg
     2281 tgatcgttgg cgaacgcgac atgctggccg ccgggcttga aaccccagac gggcgcctga
     2341 agcgccaggt tggtcgaggc gggaagcgtc cgggcgtaca ccgtctcgct gggctcggac
     2401 cggttgccgg cggcgtcacg ggctcggacg aagagctcat aggttgtagc cggagcgaat
     2461 ccggtgagcg tggcggtcag ggcggaggtg gcggccgcca cgacgccatc ggcatggatt
     2521 tcgtaacccg tgaccgcgac atcatccgat gccggcgacc aggcgagcgc aatcgtcgcc
     2581 tccgtgaccg tggtcacggt gagcgcatcg ggcggggatg gcggcgtcgg gtcgccggcg
     2641 ggggcgtggg cgagcgaagc gaaatcccgc tgcaggcccg cctggcggat gatcgaggcg
     2701 ggggcgatct ccggctcctc gaaccagacg ttgtcgcgaa tgtcccaggt ctccacgccc
     2761 caggcggtcg ggacgacgcg ggtgagccgg ccgccctggc gatgctggct ggtggagaaa
     2821 ttgcgccgga tctggtggtc ggagcctttc accatcaggt cgaacgtctg cccgtgcatg
     2881 acgttttgcg tcacaacggc atagcggctc ccctgatcga gatagacgcc ggccttcacg
     2941 tcgcccaggc gcgtgccggc atcatgaatg tagttgtgcg cgatgaggag tcccggctgc
     3001 cagcccagcg agtagatgcc ggcgccatcg ttcatccgct gcatgtaatg atgaatgtga
     3061 ttatgggaaa aggtattgtt attggaaatg gacgggtccg cttcgctgtc gacaagtccg
     3121 aacccccagc cgttgctgat tccggaatag gggatatgac agatttcgtt gtgcgcgatc
     3181 agggttgaat ccgtgtagaa gttggttatg gcgggacacc cctgatactc aacgccgatg
     3241 tcatggatgg cgttattggt gatctggttg ccgcgatcgt aatcggccgg gctggaggcg
     3301 tgatgcctgg tgaagaagcc aatttgaatc gccgatccgg agatgtcgtg aaagtgattt
     3361 ccgttgatca cgttgtcctg cgagccatcc aggaattcga gcgccacggc gcccaaccgg
     3421 gtgaatgtat tgcctgaaaa cgcgatgttg ctggcggcgt gaaacgtcag attgcccggc
     3481 atttgcatcg tggcggcgac gtcccccttg cggagcgtcc cctgcacatt cgcccagcca
     3541 agatcttcat tggggccaag ccaggtggcg tgcgagaacg tcagcccctt gaacaggatg
     3601 tgatgcacct tgttgtttag ctggcccgct ccccggacca gggccgtgag tcggggggcg
     3661 atgacccgcg ccgaggccag ctgttcgccc gcgagcggct tgtaatagaa cacgtcttcg
     3721 gcgcgatcca ggtaccattc cccttcttcg tccagcagtt cataggcgtt ttccatgtag
     3781 agcggccgat caacggagaa ggcgccccaa ctgttgcagt ggctccaaca gggatcttgc
     3841 atgctgacaa gatggccggc gatggcgcga acggggcagc gcggttgacg ccattccacc
     3901 tcaaacacga attccagatc ggtgatgttg ttccagtgct gcatcccgag gtcggtggtc
     3961 ccgtagcccg cggccgtctt gaccgcgccg ggcaggccgc cttgcgaacg agcgcggacg
     4021 gcgcgccggc cctcgacata gagctggcgc gtgttgacgc ccctggcggg ggcgcgccag
     4081 atgtcgcgcg cggcgtcatg aagcgtccag ccgtcgatca gcacgccgcc gctgattacc
     4141 ggcgactcac cggggtaagc ctcgtaacgg acccagtggc cattcccgcc ggaatcgagc
     4201 ggggtcaagg tgaggggctc cgccaggaaa tgcaggccgt ccctcaggta gacgacgata
     4261 tcgtccgtca tggcgccgtt ggccgcgcgg accgcctgct gggctcgggc cagggattgg
     4321 aagggctgat cgatggtgcc atccgccgcg tcgctccccg tggcggcgac ataaaaggtc
     4381 ggctcggcgg agagcggcat gatcaatgca agctgagcga cgaggcagat gagaaacagc
     4441 cgagaggcga tggcggggat tcgaataggc atgacgcgcg cggcctcggg cgcggcgtcc
     4501 gtctcctggc gcagggtagg cgccagattc agccagagac gcaggagcgg atccctctcg
     4561 tcgcgcggac ggctttcgcg cagggcctcg accaggactg ccagtacgat gcgcctttcg
     4621 ccgggctgga ggccgccgat caggtcgccg atgcggcgca ggcccgcctc gcgggcgcgc
     4681 gggtcgccgg agaagagggc ctgcatggcg tcggcggtgt cgcggtgaaa gcccagttgc
     4741 tccatggcgc ggatggcctc ggcgcgcacg gggccggcgg agtcgagcag cgcgcgcagg
     4801 cagatgccgc cgcgctcctg gtcggaaagg ggggcataga tcaacttgcg cagcgccgac
//
LOCUS       DAOPCO010000302         4858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14640_length_4858_cov_4.588174, whole genome shotgun sequence.
ACCESSION   DAOPCO010000302 DAOPCO010000000
VERSION     DAOPCO010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14640_length_4858_cov_4.588174"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLS90_15170"
     CDS             <1..331
                     /locus_tag="PLS90_15170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPK03787.1"
                     /translation="QGWWRRRRRDQTYSLHDLSGGHAEEEQAFDPPDTAPSPRQIAAG
                     REEVSALETAMGRLSPEHNEVLMLRFADGQSYEEIAETLQISLGTVKSRINRARRELR
                     ARLDELM"
     gene            360..1934
                     /locus_tag="PLS90_15175"
     CDS             360..1934
                     /locus_tag="PLS90_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03788.1"
                     /translation="MNEDFDRYLEAEWAGELDAAGRARLDALCAADPALAERRRRESE
                     LTALLARQGPNHAPGGLARAVFARIDAEAAAGSRAAAGGWRGWLRGLASPWWRAPQLR
                     WGMAFALVMVAVVEVAVILRGGRGEAPMVAMRSPETDAGADTAAAPGISASRQQELME
                     LASQVRVNTLHETPAGPPAAIQAEQTRPAPAEPVDATAIAAAPAKPADEPTITAEEPA
                     ATLAAAETRQAEAPEADRVTVAASESASPAEPEPESIESVPAVPAPGEEPAPATERAV
                     ARADAAPGEPEAAESATPESALAFAAERSEEPARAPVARADRAADARDADAPVAAEEP
                     RMTIRIRLADAAPTETERAEPRDERWTNFLTQLPRDGAEALRTERRQGFAPRPTAPAS
                     RRPTARQQVRLDDLALGVAEAGGEIVAPPQAVRGRDGVWRVMARMTPQEMEDFQQWLR
                     RRGIERSSDALEKTARGGRSVYEIVEDGAPVRAVAADELDDRPSTPAGTEGERRARSN
                     RRMVLVLVESLKANGE"
     gene            2095..2904
                     /locus_tag="PLS90_15180"
     CDS             2095..2904
                     /locus_tag="PLS90_15180"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HPK03789.1"
                     /translation="MPSFISGLNLFDGGKQVRRYRLISCEVAYRECCLAVAVSPNVID
                     LHFLTQGLHDLQSKSMCERVQQAVDETPAGRYEAILLGYGLCNNGLVGVTARHTPLVL
                     PRAHDCITLFLGSKEKYAEQFAQNSGTYYLTTGWVERDHENLENTHEREDNIMHKMGL
                     DKSYEEYAALYGEQAAKMIFATIGGMQHYREIGYIDMGVAPLAEEHVQTKASAEAQRR
                     GWTFNPRRGRMDLFLELTRGDWDESKFLIVPPGHRIEQSYDESIVKAAPAA"
     gene            2923..3369
                     /locus_tag="PLS90_15185"
     CDS             2923..3369
                     /locus_tag="PLS90_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HPK03790.1"
                     /translation="MAFDPAVLPPINAALNATAGLLLLAGWAAWRRRRLHLHARLMVS
                     AFAVSTLFLISYLTYHASAGHTTFPDLGWIRGVYLLILATHVILAAVVAPLAVAALVL
                     ALRGRFARHRRLTRWLLPLWLYVSVTGVIIYFMLYHWFAAAGRPIP"
     gene            3562..3792
                     /gene="rpmB"
                     /locus_tag="PLS90_15190"
     CDS             3562..3792
                     /gene="rpmB"
                     /locus_tag="PLS90_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPK03791.1"
                     /translation="MMANCVVCGKGPQVGHSVSHANNKTKRRWLPNIQKIRINWEGRV
                     RMAPVCASCLRSGKVTKAARGVNAARTQPSAS"
     gene            complement(3936..>4858)
                     /locus_tag="PLS90_15195"
     CDS             complement(3936..>4858)
                     /locus_tag="PLS90_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03792.1"
                     /translation="DWLGQGHGLFVSGTAPTYVSVMGLEPTSTRVVGGGGQDGFVAAV
                     AAGDYSRHPVYEGFDTNAPIPLVGGGYPPFSDFYGSGGPQAGNPIGEAAPDAGEHPLV
                     EFACGQGRVIAMGWRLPHYGLRPNAHRANLERLTGNVLCYLASGDWYGTIEDGRTRAL
                     RAELERITGMMPHACRYAPHLRRGGRLANPFDDDGPRNWLLETDEDGRCLFAYTNADG
                     ETRCSIHSAALDLGLEPYHVKPRACALWPLALSEDRPPVLSIQNDCFDFPCNTRRRGR
                     ARTLDPGIAELIERNFGKPFLDKLHRLITG"
BASE COUNT          755 a         1603 c         1818 g          682 t
ORIGIN      
        1 ccagggctgg tggcggcggc ggcggcgcga ccagacctac tcgctgcacg acctgagcgg
       61 cggccacgcc gaggaggaac aggcgtttga tccgcccgac acggccccct cgccgcgcca
      121 gatcgcggcc ggccgcgagg aagtcagcgc cctggagacg gcgatgggcc ggctctcgcc
      181 cgagcacaac gaggtgctga tgctgcgctt cgccgacggc cagagttacg aagagatcgc
      241 cgagacgttg cagatttcgc tcggcacggt caaatcgcgg atcaaccgcg cgcggcgcga
      301 gctgcgcgcc cggcttgatg aactgatgtg atgaacgccc gagaggaacg ataccgaccg
      361 tgaacgaaga cttcgacaga tacctggagg cggagtgggc cggcgagctg gatgcggcgg
      421 gccgggcgcg gctcgacgcg ctctgcgcgg ccgatccggc gctggccgaa cgccgccggc
      481 gcgagtcgga gctgaccgcc ctgctggccc ggcaggggcc gaatcatgcc cccggcgggc
      541 tggcccgcgc cgtgttcgcc cgcatcgatg ccgaggcggc ggccggctcg cgcgcggcgg
      601 ccggcggctg gcgcggctgg ctgcgcggtc tggcgagccc gtggtggcgc gcgccgcaac
      661 tgcgctgggg catggcgttt gcccttgtca tggtggccgt ggtggaggtt gccgtgattc
      721 tgcgcggcgg ccgcggcgag gcgccgatgg tcgcaatgcg ttcgcccgag acggatgcgg
      781 gcgccgacac ggcggccgcg cccgggatca gcgcgtcccg ccagcaggaa ctgatggaac
      841 tggcgagcca ggtgcgggtc aacacgctcc atgagacgcc ggcgggtccg ccggccgcaa
      901 tccaggcgga gcagaccaga ccggcgccgg cggagccggt ggatgcgacg gcgatcgcgg
      961 cggccccggc gaagccggcg gacgaaccga ccatcacggc ggaggaaccc gcggcaacgc
     1021 tcgcggcggc tgagacgcgg caagccgagg cgccggaagc ggatcgggtg accgttgcgg
     1081 cgagcgagtc agcttccccg gccgaaccgg agccggaatc gattgaatcc gtccccgcgg
     1141 tcccggcgcc gggggaagag ccggccccgg cgaccgagcg cgcggtcgcg cgggcggacg
     1201 ccgcccccgg cgagcccgag gcggcggagt cggcgactcc tgagtccgcc ctggccttcg
     1261 cggccgagcg cagtgaggaa ccggcgcgag cccccgtggc gcgagcggac cgcgcggccg
     1321 acgcccgcga tgcggacgcg ccggtcgccg ccgaggagcc gcggatgacg atccgcatcc
     1381 ggctggccga cgcggccccc acggaaacgg aacgcgccga accgcgcgac gaacgctgga
     1441 cgaattttct gacgcaactg ccgcgggacg gggccgaggc gctccgcacc gagcggcggc
     1501 agggcttcgc cccgcggccg acggcgcccg cgtcgcggcg ccccaccgcg cgccagcagg
     1561 tccggctgga cgacctggcg ctgggcgtgg ccgaggcggg gggtgagatc gtcgcgccgc
     1621 cgcaggccgt gcgcggccgc gacggcgtct ggcgcgtgat ggcgcggatg acgccgcagg
     1681 agatggagga ttttcagcag tggctgcgcc ggcgcgggat cgagcggagc agcgatgcgc
     1741 tggagaaaac ggcgcgcggc ggccgttcgg tctacgagat cgtggaagac ggcgcgcccg
     1801 tccgcgcggt ggcggccgac gagctggacg accggccgtc gactccggcc gggaccgaag
     1861 gggagcggcg cgcccgctcg aaccgccgga tggtgctcgt gctcgttgag tcgttgaagg
     1921 ccaatggcga ataaaggaca taagcgacac aagggacagc agggtgaccg gtcacgagtt
     1981 tgagaggagc ctccgggtgg atcacgaggc gctgaattca tgatgccatt atccattgag
     2041 tcggaccccg atatccgcta aagtaaaccc gactttgcgg aggggtttga catcgtgcct
     2101 tccttcatca gcgggctgaa tctgttcgac ggcggcaagc aggtccggcg ctatcgcctg
     2161 atctcgtgcg aggtcgccta tcgcgagtgc tgcctggcgg tggccgtctc gcccaacgtc
     2221 atcgacttgc atttcctgac ccaggggctg cacgatctgc agagcaaatc gatgtgcgaa
     2281 cgcgtgcagc aggcggtgga cgaaacgccc gccggccgct acgaggcgat cctgctgggt
     2341 tacgggttgt gcaacaacgg gctggtcggc gtaacggccc gccacacgcc gctggtcctg
     2401 ccgcgcgccc acgactgcat cacgctgttc ctgggctcca aggagaaata cgccgaacaa
     2461 ttcgcccaga acagcggcac gtattacctg acgaccggct gggtggagcg cgatcacgag
     2521 aacctggaga acacccacga acgcgaagac aacatcatgc acaagatggg gctggacaag
     2581 tcgtacgagg agtatgccgc gctgtacggc gagcaagccg ccaagatgat ctttgcgacg
     2641 atcggcggga tgcagcacta ccgcgaaatc ggctacatcg acatgggggt tgcgccgctg
     2701 gccgaggagc acgtgcagac caaggccagc gccgaggcgc agcgtcgcgg gtggacgttc
     2761 aacccccggc gcggccggat ggatctgttc ctcgagctga cgcgcggcga ctgggacgag
     2821 tcgaaattcc tgatcgtgcc gccgggccac cgcatcgagc aatcgtatga cgagtccatc
     2881 gtgaaggccg cccccgcggc gtagaggggg gagccggtcg cgatggcgtt tgaccccgcg
     2941 gtgctgccgc cgatcaatgc ggccctgaac gccacggcgg ggctgctgct gctggcgggc
     3001 tgggcggcct ggcggcggcg gcggctccac ctgcatgccc ggctgatggt gtcggcgttc
     3061 gccgtttcga cgctgtttct gatcagctac ctgacctatc acgcttcggc cggccacacg
     3121 acgttccccg atctgggttg gatccgcggg gtctacctgc tgatcctcgc gacgcacgtg
     3181 atcctggcgg cggtcgtcgc gccgctggcg gtcgcggcgc tcgtcctggc gctgcggggc
     3241 cggttcgccc gccaccgccg gttgacccgc tggctgctgc cgctgtggct ctatgtctcg
     3301 gtcacgggcg tcatcatcta tttcatgctc tatcactggt tcgccgccgc cgggcggccg
     3361 atcccgtgat tcgtttccct gtcgggggaa ccccgaaaat cgccggtgtt ttcaccggtc
     3421 aacatttggt tgaccgccgc gggcggggat cggtaggatg ttggtttcgc gcgcccgcgc
     3481 cggggtgcgc ggattcggta tcggccagcc ggcgggcgcc ggcggaacaa gtcatccgaa
     3541 catcagccac gaggaacggg catcatggcc aactgtgtcg tctgcggaaa aggcccccag
     3601 gtgggtcaca gcgtctcgca cgccaacaac aagaccaagc ggcgctggct gccgaacatt
     3661 cagaagatcc ggatcaactg ggaaggccgg gtgcggatgg cgccggtttg cgcgagctgc
     3721 ctgcgctcgg ggaaggtgac caaggcggcg cgcggagtca acgcggcgcg gacgcagccc
     3781 agcgcctcgt aggcggccca gcctcccatt gtaacagcat gccccgtccg gttcgccagg
     3841 aaccggacgg ggcgtttcgt ttggggggcg gggttcgcca ccctcatgcc aggaaggggc
     3901 aggatgccgt cgatccaggc tgccggcggg ggaggtcagc cggtgatcag gcggtggagt
     3961 ttgtccagga agggtttgcc gaaattgcgt tcgatcagct cggcgatgcc ggggtcgagc
     4021 gtgcgggcgc ggccgcggcg gcgcgtgttg cacgggaagt cgaagcaatc gttctgaatg
     4081 gacaggaccg gcgggcggtc ctcgctgagc gcgagcggcc acagcgcgca cgcccggggc
     4141 ttgacgtggt acggctccag gccgagatcg agcgcggccg agtggatcga gcagcgggtc
     4201 tcgccgtcgg cgttggtgta ggcgaacagg cagcggccgt cctcgtcggt ttcgaggagc
     4261 cagttgcgcg ggccgtcgtc gtcgaacggg ttggcgagcc ggcccccccg gcgcagatgc
     4321 ggggcgtagc ggcaggcgtg gggcatcatg ccggtgatgc gctccagttc ggcccgcaga
     4381 gcccgggtac gaccgtcctc gatggtcccg taccagtcgc ccgaggccag atagcacagg
     4441 acattgccgg tgagacgctc caggttggcc cggtgcgcgt tgggccgcag accgtagtgg
     4501 ggcagacgcc agcccatggc gatgacccgc ccctgcccgc aggcgaactc taccagggga
     4561 tgctcgccgg cgtccggcgc cgcctccccg atgggattgc ccgcctgtgg cccgccgctg
     4621 ccgtagaagt cggagaacgg cgggtacccg ccgccgacca aaggtatggg cgcgttggtg
     4681 tcgaagccct cgtacacggg gtggcggctg tagtccccgg ccgccaccgc ggccacgaat
     4741 ccatcctgcc caccgccgcc gacgacgcgc gtggaggtgg gctccagccc catgactgag
     4801 acgtaggtcg gcgccgtccc tgagacgaag agcccgtgcc cctgccccag ccagtcgc
//
LOCUS       DAOPCO010000303         4804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14814_length_4804_cov_4.740156, whole genome shotgun sequence.
ACCESSION   DAOPCO010000303 DAOPCO010000000
VERSION     DAOPCO010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14814_length_4804_cov_4.740156"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="PLS90_15200"
     CDS             <1..1300
                     /locus_tag="PLS90_15200"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPK03793.1"
                     /translation="KIENFNAQPLPDPIKQTWALCDNPPIRLGVFQRDPRRPDGGYPV
                     MEGLTVQSLYRPKPWEWTFNHGARIFHANGRFYASWSNNYMHEPGIGSRVLGTSSTDG
                     KAWLPAYIVFPSMGGNVSDKMDNVFMEGMGVGVPEASPFMEVNGRIYAIGIVSARNFK
                     GHDGELGMLAREVTEPEKPGPIFWIADRVPPLGEQYPAFVQLGEPHIAEDVAAILKDL
                     NLEQVPGAIRYADLRSAVRGENPLAADGGIIDHDPIYTCRDGAQIRIFRNYNGTHRLY
                     ASARKNEDEEWGLAIPTNIPDSPSGVAVGELPDGQVYLIGNQIAGELDDPDARPYRHA
                     GKLWPFAERDFQPEHFPRDPLTLAISQDGYLFDRMWAIRWDCPIPRHNALGWIPGYQY
                     PHAIVVGETLWVICSVNKEDIEVMQIPIARVNEKVNGIVE"
     gene            1511..4336
                     /locus_tag="PLS90_15205"
     CDS             1511..4336
                     /locus_tag="PLS90_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK03794.1"
                     /translation="MLFLVVISWRAEGQTVWRESTFEDFRDGSFSDGGANTYVSANGR
                     IQTINRYDANGDGYIDLLFANSHSYAERPDMTIYWGNGRDFDIRRHVRVPADGPRWIA
                     PADLDGDGAMDLVVANRDNGTYSEMDLFLYYGGTDSPEREPAAEDWSCYPFERKETLP
                     NKAPGKPATGDLDRDGDLDIVIPYSSGFSMSGQDTGEGDQTRQISRIYWGEGGRFDGE
                     RFTELPTKGANAAAVADLNGDDWPEIIFANGEEDVSFIYWNGEAGFLENQVTALPTHG
                     AHSVAVGDLDNDGLPDVAFANQEGEESWAYLNRDGSFDAAQRIAFETQMALDCVIEDF
                     NRDGMADIFFSNHEVNGNRVTQSFLYYGSGDGFVNDNRLELTTIGAWGASAADLNGDG
                     WPELVVSNFQDRYTYEVPSFVYWNSPRGINEGMRTPLFEHGARGNAIADFNGDGFLDI
                     AFANTFTLSANDYHPNYLYYGNEHGSYSAANRIELLGLESQGRGMADLDDDGDVDIVF
                     GNQGEGHDVLWGVNDIFIYWNEDNQYDFGRRTGLPAYQINGLLLSDMDRDGYIDIISA
                     NHRSYPGATGIDPGVVIYWGGPDGFIVSERSALPADRVRAPAVADLDRDGNLDIIFGN
                     EGDQEGSIFYGDGTRSTEQWRQVFIPGTQKTGYPNIADLNRDGFLDLIMTRGGSADET
                     FSIYYGNERGEYTRENAVEIGTQAKTVTVADVNRDGWLDIVCAVYWHRTGRDTISHIL
                     LGSASGFSMKRKISLPTNSSDGSIVSDFNLDGYNDVFFYCHRSQGDVNRPGMPGDHKT
                     ASFLYWGGAAGFQADRRLEIPGLGVHARKGSDDLGDIYNRKYDYEYISSPYELGEGRP
                     LSIDWSAREPHRSAVRFQVRAARTEEELGDAEWIGPAAGGDYITEHNESLSHLPASAR
                     WIQYKAILHTDNGAYSPVLESVEIRIE"
BASE COUNT         1089 a         1254 c         1447 g         1014 t
ORIGIN      
        1 caagattgaa aactttaacg ctcaacctct ccccgatccg atcaagcaga cgtgggctct
       61 ttgtgacaat ccacctattc ggttaggggt ctttcaacgt gatccgcgcc gtccggacgg
      121 cgggtatcct gtcatggagg ggttgaccgt tcagtcgctc tatcggccca aaccgtggga
      181 atggacgttc aatcacggtg cgcgaatctt tcatgccaac ggccggtttt atgcgagctg
      241 gtcaaataat tacatgcacg agcccggtat cggcagccgg gttctgggca catcgtccac
      301 ggatggaaaa gcgtggctgc cggcatacat tgtatttcca agcatgggcg ggaacgtcag
      361 cgacaagatg gacaatgttt ttatggaggg gatgggggtc ggagtcccgg aagcctctcc
      421 attcatggaa gttaacgggc gtatttatgc catcgggatt gtatcggcca gaaattttaa
      481 agggcatgac ggcgaactgg gcatgctggc ccgggaagtg acggaaccgg aaaagccggg
      541 accgatcttc tggattgccg atcgtgttcc tccgctggga gagcaatatc ccgcgttcgt
      601 gcaactgggg gagccgcata tcgccgaaga tgtcgcggcc attctgaaag acctcaatct
      661 ggaacaagtg ccaggggcga ttcgctacgc ggacctgcgc agtgccgtcc gaggggaaaa
      721 cccgctggcg gcggatgggg gaatcatcga ccacgacccg atctatacgt gccgcgatgg
      781 agcccaaatt cgaatctttc gcaactacaa tggcactcac cggttgtatg cgtcagcccg
      841 gaaaaacgag gacgaggaat gggggctggc gatcccgacc aatatccccg attcaccttc
      901 gggcgtcgcc gtcggcgaac ttccggacgg ccaggtctac ctgataggta accagatcgc
      961 cggcgaactg gatgatcccg acgcccggcc ctatcgtcac gccggaaagc tgtggccatt
     1021 tgccgaacgc gatttccaac cggaacattt tccacgtgac ccgctgacgc tggcgatcag
     1081 tcaggatgga tatctctttg acagaatgtg ggcaatccgc tgggattgtc cgataccgcg
     1141 acataatgca ttgggatgga ttcccggcta tcagtaccct catgccattg tcgttgggga
     1201 gacgctatgg gtcatctgtt ccgtcaataa ggaagacatt gaagtgatgc aaattccgat
     1261 cgctcgagtc aatgaaaaag taaatggaat agtcgaataa cgatgcaata tccggcaagc
     1321 tcatcgcaat ggaatgcaag agaatcagcc agccctgcaa cgttgtgtga tgaggcgcct
     1381 cccgtactga acgaacgatg tggagaagtc ctgattgcgc tgaattaatc gctagaaacg
     1441 taataattgc catattcaat cctcatcgtg aggtactaca aagatgcgag ctgcttccgc
     1501 gacagcatgg atgctattcc tggttgtgat ctcatggcga gcagaagggc agacagtctg
     1561 gcgtgaaagc acctttgagg acttccgtga tggttcgttc agtgacggcg gagctaatac
     1621 ctacgtttcg gctaatggac gtattcaaac gatcaaccgt tacgacgcca acggcgacgg
     1681 atatatcgat ctcctgttcg caaactcaca ttcctatgcc gaacggccgg acatgacgat
     1741 ttactggggc aacgggcggg atttcgacat ccgtcgacac gtgcgtgtgc cggccgacgg
     1801 gccgcgctgg atcgcgcccg cggatctgga tggcgacggt gcgatggatc tggttgtggc
     1861 taatcgcgac aacggcacct attcggaaat ggatctgttt ctgtactatg gcgggactga
     1921 ttcgcccgag cgggaaccgg cggcggaaga ttggagttgc tatccattcg agcggaaaga
     1981 aacattgccg aacaaggccc ccggcaaacc cgcaacgggc gatctggatc gcgacggaga
     2041 cttggacatc gtgattccat actcaagcgg gttttcgatg tctggacagg atacggggga
     2101 gggcgaccag acccggcaga tctcgcggat ttactggggc gaggggggca gatttgatgg
     2161 tgagcgattc accgaactgc ccacgaaggg ggcgaatgcc gcggccgtgg cggatctgaa
     2221 cggcgatgat tggcccgaga ttattttcgc caacggcgag gaggacgttt catttatcta
     2281 ttggaatgga gaggccggct ttttggagaa tcaggtcacg gcgctcccca cccacggcgc
     2341 ccattccgtg gccgtagggg atctggacaa tgacggcctg ccggacgtgg cctttgcgaa
     2401 tcaggaaggc gaagagtcgt gggcctacct taaccgcgat ggatcattcg atgccgctca
     2461 acggattgcc tttgagacgc agatggccct ggattgcgtc atcgaggatt tcaatcgcga
     2521 cgggatggcg gatatattct tcagcaatca cgaggtcaat ggtaaccggg tgacgcagtc
     2581 gtttctatat tatggatccg gcgatggttt cgtaaatgac aatcgtctgg aattgacaac
     2641 gatcggggcg tggggcgctt cggcggccga tcttaacggc gacggatggc ccgaactggt
     2701 cgtcagcaat tttcaggacc gatatacgta tgaagttccg tcgtttgtct actggaattc
     2761 accgcgcggg atcaatgagg gtatgcggac cccgctcttc gagcacgggg cgcgaggcaa
     2821 cgcgattgcc gatttcaacg gcgacggctt tctcgatatc gcctttgcga acacgttcac
     2881 cctttccgcc aacgattacc atccaaatta cctgtattat ggaaacgagc atggctccta
     2941 tagcgcggcg aaccggatag aattgctcgg cctggaatcg cagggtcgcg gtatggccga
     3001 cctggatgat gacggagacg tggacattgt cttcggaaat caaggcgagg ggcatgacgt
     3061 cctgtggggc gtgaatgaca tattcattta ttggaacgag gacaaccagt acgacttcgg
     3121 gcggcggacg gggctgcccg cctaccagat caacgggttg cttctctccg acatggatcg
     3181 cgatgggtac atcgatatca tctcggccaa tcatcgatcc taccccggcg ccacggggat
     3241 cgatccagga gtggtcatct attggggagg cccggacgga ttcatcgtct cggaacgatc
     3301 ggctttgccg gctgaccgcg tgcgagcgcc ggccgtcgct gatctggatc gagacggaaa
     3361 tctcgacatt atattcggca acgaggggga ccaggagggt tcaatattct acggcgacgg
     3421 gacgcgcagc accgagcaat ggcgtcaggt ttttatcccg ggaacgcaga agaccggcta
     3481 tcccaatatc gccgacctga atcgtgatgg atttctggat ttgattatga cccgcggcgg
     3541 aagcgcggac gagacttttt cgatttatta tggaaatgag cgcggagaat acacgaggga
     3601 gaacgcggtc gaaatcggca cgcaggctaa gaccgtgacg gtggccgatg tcaatcgcga
     3661 cggctggctc gatatcgtct gtgcggtgta ttggcatcga acgggccgcg acacgatctc
     3721 ccatattcta ttggggagtg cctcaggatt cagcatgaag cggaaaatct cgctgcccac
     3781 caacagcagc gatgggtcca tcgtgagcga ttttaacctc gacggttaca atgatgtgtt
     3841 cttctactgt caccgcagcc agggcgacgt caaccgtccc ggcatgcccg gcgatcacaa
     3901 aacggcttca tttctgtact ggggcggagc tgcggggttt caagccgatc gacggttgga
     3961 gatccccggc ctcggcgtcc acgcccgcaa aggcagtgat gatttgggcg atatttacaa
     4021 tcggaaatat gattatgaat acatatcatc gccatatgag ctcggcgaag gccggcccct
     4081 gtcaatcgac tggtcggcgc gggaacctca tcgcagcgcc gtccgcttcc aggtacgtgc
     4141 ggcgaggacg gaagaggaat tgggtgatgc cgagtggatc ggtccggccg cggggggaga
     4201 ctatatcacc gaacacaatg aatcgctgtc gcatttgcca gcgtcggctc gatggatcca
     4261 gtataaggcg attcttcata ccgacaacgg cgcctactca cccgtcctgg aatcggtcga
     4321 gatccgcatt gaatgacaat acaacggcct aagcgccgat gccgtcctcg ttcaaatcgc
     4381 ttggcaaacc gtcctcaacc ggccgctacg agctgaagcg gttgacctcg ccttcggggt
     4441 agagatgctt agtgtgatca gagatcgctt cgggcgagac gttaagagat ccgtcatacc
     4501 cggagtcatg taagcataac taaactagac ggtatctgag aacagaagaa ttatccaatt
     4561 ggagagagtg gcagtggaag ccgcaagaga tgcttctgcg gccacgggct gccttgcggt
     4621 ccgatgctcg gagacgcgcg ttcaatatga gatcgggccc tgcgcatggg gcaatcctcc
     4681 ggacgcggag gaaggcgcca acgcgccaga tctgaattga catcgcggcg ccttcgttct
     4741 aaagattacc tcgagcgaaa tcgatttcct tgggcatatt gaactacatt cccacgcccc
     4801 cggc
//
LOCUS       DAOPCO010000304         4778 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14887_length_4778_cov_4.541393, whole genome shotgun sequence.
ACCESSION   DAOPCO010000304 DAOPCO010000000
VERSION     DAOPCO010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14887_length_4778_cov_4.541393"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLS90_15210"
     CDS             complement(<1..599)
                     /locus_tag="PLS90_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPK03795.1"
                     /translation="MTESLTIAFHGAAGSTTGSRHLLTIDQIPILLDCGLYQGRRKDT
                     WEHNTHFGFDPRAIHTTLLSHAHIDHSGNLPTLVKQGFQGPIHCTAATADLCRVMLLD
                     SAFIQEKDAEWLNKRKRKRGEEPIEPIYTIQDATRSLNRFETLDYHHPHHILRDVKVN
                     FYDAGHILGSASIILDIRRDGGEVRFAFSGDIGRAGLPIL"
     gene            complement(596..1306)
                     /locus_tag="PLS90_15215"
     CDS             complement(596..1306)
                     /locus_tag="PLS90_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPK03796.1"
                     /translation="MPRRQPSLQTTTLWDFPAQQYGDETQGSKDYRGATPAYVVWNLL
                     QRYTRPGNLVVDPMAGSGTTLDVARELDRKALGYDVHPSRPDITYCDARSLPLPAASV
                     DFVFIDPPYSTHVDYGDDPADIGKLHAADSAYYAAMGEVIAEIDRILRPERHMGLFCC
                     DSFEKGRPLHPIGFELFSRLRRHFEPVDIISVVRRNRTLLRNRWHEAAIAGNYYLRGF
                     NYLFIMRKPPSRRKSPRP"
     gene            complement(1558..3306)
                     /locus_tag="PLS90_15220"
     CDS             complement(1558..3306)
                     /locus_tag="PLS90_15220"
                     /inference="COORDINATES: protein motif:HMM:NF023260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HPK03797.1"
                     /translation="MSSKIMERAFRSIGILALAVAAVVAAPSSVADLPHAEAIGADAL
                     VVIGIDDTRDFWSKVEALPVAIALRQWFAGLTMQQDLDFQAFQLEVMKISEALGYEAS
                     IKELMSSVVDNALLVIGGGVQAAEPTFQLRMGINDEAKARKLIDVTTQRLREKVTQAA
                     DAAGTDTEELSQFNETEIEGTTAYHYAKPDGTEVLYGIADDMLLIANSQPAFQSMVAG
                     AEPAQKLTDTENFRKTTGAIDWDDADFTVFLDADALASSGGMQLPGLQQAGKAAIAGR
                     ILPNAIEAEAVTTYNQLEGSTTAKAGDLKGTFAASPQPLIAVDYGIFDGRKTLNQLRE
                     MMSAAQMSGFDLDSMLQSFESQTGISIEQELIPALGEDIVFALNGLQPNPMMPIFPSV
                     DLVLGAEVADEAKMQTVITKVEALLQQAFAGMSMMMQGQTGADAAPQGPQFTTMNVEG
                     VDVRTITTGLPGLSPSHASVDGYWVISPSLEGLQAALARKRSGQDVSTSPIYNELAGR
                     AGDDPRLSYSEVNIARILLAVMPMISPYLGDTSDPDTAKGLSFVNDVMSRVGSMASTD
                     VLVDGMNKSYIKVQFQ"
     gene            complement(3515..3967)
                     /locus_tag="PLS90_15225"
     CDS             complement(3515..3967)
                     /locus_tag="PLS90_15225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HPK03798.1"
                     /translation="MMRKNLMGLAALALLTVGASAFAAGTEVYREQTVANQALHKLGR
                     GVTNVLTFWAEVPRQIAIEWERTDPASGLIVGTIKGAGWGFARLATGVYDAFTFPFPI
                     PENYEPMMEPEFVITDIWGDPIPGLTEFNPNNPERPGSGPVYPQQFSF"
     gene            complement(4027..4467)
                     /locus_tag="PLS90_15230"
     CDS             complement(4027..4467)
                     /locus_tag="PLS90_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HPK03799.1"
                     /translation="MKRIVCLLWMAALTVGVGSVRADEAIWMDDERENVELISSHPSS
                     ASPSTTKGPFYYQSENELRMRKLARGAANIGLCVAEIPNQMFQEAYKTSPVTGIFVGA
                     VKGVVKGAKRLVIGAWEVATFYHPTRNYYAPYVEPEVVFQEYLH"
BASE COUNT          804 a         1513 c         1609 g          852 t
ORIGIN      
        1 aggatgggga gcccggcgcg gccgatatcg ccgctgaagg cgaaccggac ctcgccgccg
       61 tcgcgacgga tgtccaggat gatcgaggcc gagccgagga tatggccggc gtcatagaag
      121 ttgactttca cgtcgcgcag gatgtggtgc gggtgatggt aatcgagcgt ttcgaaccgg
      181 ttaagcgagc gcgtcgcgtc ctgaatcgtg tagatcggct cgatcggctc ctccccccgc
      241 ttgcgcttgc gtttgttgag ccactcggcg tccttttcct ggatgaaggc gctgtcgagc
      301 agcatcacgc ggcagaggtc ggcggtggcg gcggtgcagt ggatcggccc ctggaagccc
      361 tgcttgacca gcgtcgggag gttgccgctg tgatcgatgt gggcgtgaga caggagcgtg
      421 gtgtgaatcg cgcgggggtc gaagccgaag tgggtgttgt gctcccacgt atccttgcgc
      481 cgcccctgat acaggccgca atcgagcaga atcgggatct gatcgatggt gagcaggtgg
      541 cgcgagccgg tggtcgagcc ggcggcgccg tggaaggcga tggtcagtga ttcggtcatg
      601 ggcgcgggga ctttcggcga gatggcggct tgcgcatgat aaacaggtag ttgaagccgc
      661 gcagatagta atttcccgcg atcgccgcct cgtgccagcg attgcgcagc agcgtccgat
      721 tgcgccgcac cacggagata atgtcaaccg gttcgaaatg ccggcgcagg cggctgaaca
      781 gctcgaagcc gatcggatgg agcggccggc ctttctcgaa tgagtcgcag cagaacagcc
      841 ccatgtggcg ctcggggcgc aaaatgcggt cgatctcggc gatgacctcc cccatcgccg
      901 cgtagtaggc cgaatcggcg gcgtgcagct tgccgatgtc ggcgggatcg tcgccgtagt
      961 cgacgtgcgt cgaatagggg ggatcgatga agacgaaatc gaccgacgcg gccggcagcg
     1021 gcaggctgcg cgcgtcgcag taggtgatgt cggggcgcga gggatgaacg tcgtagccca
     1081 gagccttgcg gtccagttcg cgggcgacgt ccagcgtcgt gccgctgccg gccatcggat
     1141 cgacgaccag attgcccggg cgtgtgtagc gttgcagcag gttccagacg acgtaggccg
     1201 gcgtcgcgcc gcggtagtcc ttgcttccct gggtctcgtc gccgtattgc tgcgcgggaa
     1261 aatcccacag ggtggtggtc tgaagcgagg gctggcggcg cggcacgggc gggctccctg
     1321 acgcggggat gcgatgcggg ccgattatag cggggcgatc cggcgttgca cgggagaaag
     1381 ttctcgtccg cccgcgatgg ggtattcgga tcggaatcgg tatcgcaatc gctatcgctg
     1441 tcggcctcga tccgacccgc ctgccccaac gccgcctccg atccccgaaa cggaaacggc
     1501 cgagggagcg gatgctccct cggccgcaac acggtttctt caagcgcccg cgtgggctta
     1561 ctggaactgg accttgatgt aggacttgtt catgccgtcg accaggacat cggtggaagc
     1621 catcgagccg acgcgcgaca tgacgtcgtt gacgaacgag agccccttgg ccgtgtccgg
     1681 atcgctcgtg tcgcccagat agggggaaat catcggcatc acggcgagga ggatccgggc
     1741 gatgttgacc tcgctgtagc tcagccgggg atcatcgccg gcgcggcccg ccagttcgtt
     1801 gtagatcggc gacgtgctca catcctggcc gctgcgtttg cgcgccagcg ccgcctgcag
     1861 gccttcgagc gacggcgaga tgacccagta gccgtcaacg ctggcatgcg acggcgacag
     1921 gccgggcagg ccggtggtga tcgtgcggac gtcgaccccc tccacgttca tcgtcgtgaa
     1981 ctgcggtccc tgcggcgccg cgtcagcgcc cgtctggccc tgcatcatca tcgacatgcc
     2041 ggcgaaggcc tgctgaagca gcgcctccac tttcgtgatg accgtctgca tcttggcttc
     2101 gtcggcgact tcggccccca gaaccagatc gaccgaggga aagatcggca tcatcggatt
     2161 gggctgcagg ccgttgagcg cgaagacgat gtcctcgccg agggcgggga tcagctcctg
     2221 ctcgatcgag atgccggtct gggattcgaa gctctggagc atcgaatcca ggtcgaagcc
     2281 gctcatctgg gcggcgctca tcatctcgcg caactgattg agtgtctttc tgccgtcaaa
     2341 gatgccgtag tccaccgcga tcagcggctg cggcgaagcg gcgaacgtcc ccttcaggtc
     2401 gcccgccttg gccgtggtcg agccttcaag ctggttgtag gtggtgaccg cctcggcctc
     2461 gatcgcattg ggcaggatgc ggccggcgat ggcggccttg cccgcctgct gcagaccggg
     2521 cagttgcatg ccgcccgaac tggcgagcgc gtcggcgtcc aggaaaaccg tgaaatcggc
     2581 gtcgtcccag tcgatggcgc cggtcgtttt ccggaaattc tcggtgtcgg tcagtttctg
     2641 ggcgggctcg gcgccggcga ccatcgactg gaacgccggc tgcgaattgg cgatcagcag
     2701 catgtcgtcg gcgatgccgt agaggacttc ggtcccgtcg ggcttcgcat aatgataggc
     2761 ggtggtgccc tcgatctcgg tctcgttgaa ctggctcagc tcctcggtgt cggtgccggc
     2821 ggcatcggcc gcctgcgtca ccttctcgcg caggcgctgg gtggtaacat caatcagctt
     2881 gcgcgccttg gcctcgtcgt tgatccccat ccgcaactgg aaggtgggct cggccgcctg
     2941 caccccgccg ccgatgacga gcaacgcgtt gtcgacgacg ctgctcatca gctccttgat
     3001 cgacgcctcg tagcccagcg cttcgctgat cttcatcact tcgagctgga aggcctgaaa
     3061 atcgagatcc tgctgcatcg tgaggccggc gaaccactgc cgcagcgcga tggcgaccgg
     3121 cagcgcttcg actttcgacc agaaatcccg ggtgtcgtcg atgccgatga cgacgagcgc
     3181 gtctgcgccg atcgcttcgg catggggcaa atcggccacg ctcgacggcg ccgccacaac
     3241 cgcggcaacc gccagcgcca ggatgccgat cgaccgaaac gcgcgttcca tgatcttgct
     3301 gctcacgatc aaaaacccct ttctgtgggt caccccggcg ggtgaggctg acggcgccga
     3361 ccgaaaacgg tgcccgggcg gcggaccaag aaaatgccca cggtcgctca tgtgacgaca
     3421 cgtgggcccg tactcgaatc gtcgcgcctg cgccatggac atgctcgacg acgggaagtt
     3481 ccctcgcgga gcgacggcgc cggccgcccc gaactcagaa actgaactgc tgcggataga
     3541 ccgggccgct gccgggacgc tccgggttgt tcggattgaa ctcggtcagc ccggggatcg
     3601 ggtcgcccca gatatccgta atgacgaatt cgggctccat catcggctcg tagttctcgg
     3661 gaatcggaaa ggggaacgtg aacgcatcgt agacgccggt ggcgaggcgg gcgaagcccc
     3721 acccggcccc cttgatcgtg ccgacgatca ggccggaggc gggatcggtg cgttcccact
     3781 cgatggcgat ctggcgcgga acctccgccc agaaggtcag gacgttggtg acgccccggc
     3841 ccagcttgtg cagcgcctga ttggcgacgg tctgctcgcg atagacttcg gtgcccgcgg
     3901 cgaaggccga ggcgccgacc gtcagcagcg ccagggctgc cagacccatt aggttcttgc
     3961 gcatcatctc gctccgactc cttccggttg aagaccgctg ccgcatgcga gcgggccgcc
     4021 gccgtttcaa tgcaggtatt cctggaagac gacttcgggc tccacatacg gtgcatagta
     4081 attccgggtc gggtggtaga aggtggcgac ctcccacgcc ccgatcacca ggcgcttggc
     4141 ccccttgacc acgcccttga cggcgccgac gaaaatgccc gtcaccggcg aggtcttgta
     4201 ggcctcctgg aacatctggt tggggatctc ggcgacgcac aggccgatgt tcgccgcgcc
     4261 gcgcgccagc ttgcgcatcc gcagctcgtt ctcgctctgg tagtagaagg gacccttggt
     4321 ggtggagggc gaggcggagc tcggatggct gctgatcagc tccacgttct ccctctcgtc
     4381 atccatccag atcgcctcgt cggcgcggac cgaaccgacg ccgacggtca gggccgccat
     4441 ccagagcagg cacacgatgc gcttcacgac atcactcccc tcgcggtgcg agccggatcg
     4501 gcgcggacct gagccgcacc acggccggca accgtggagt tcctccaggt atgttccaag
     4561 tgggaccgta gcaaccccct acggcaaaag caagaaaaag taaacggggt tgccgaggcc
     4621 cttttccggt cgctcccgac cgaataaatc ggtgccaaca tcggtatcgc tttcgccatc
     4681 ggtctcggtc ccgggatcgc acccaccgcc cacccccatc ccaccgcttc atcccgccgg
     4741 gatgccggat gggagccggt ggttgaacgc agcgacac
//
LOCUS       DAOPCO010000305         4761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14943_length_4761_cov_5.943901, whole genome shotgun sequence.
ACCESSION   DAOPCO010000305 DAOPCO010000000
VERSION     DAOPCO010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14943_length_4761_cov_5.943901"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..2521)
                     /locus_tag="PLS90_15235"
     CDS             complement(473..2521)
                     /locus_tag="PLS90_15235"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPK03800.1"
                     /translation="MFKLQDMRDTKKLKWPIYIGFAIIIISFIFFYGFRPDAGRMQNA
                     DYDVARLRTGSINPLESWETISVREAARLRQDVLMEKASQLPPQMQMLLQTNPGIENR
                     LVTDRDLAYAAADKRLIQRHADEMGVDVATRDVVEMLRQQPYMTEERLEAAAMQMGMD
                     KHQFVEMIREDQVESRVRALRTMLAHASLFELWQEYLLFNEKITFQMAAYPTAEFVDQ
                     VVVTEQELQDYLAEHIDEFRVPETRRYAYVKLTREDLRDALEPTSASLHVYYEQHQVD
                     YFEPGARLVEEMYVPLLEDDTTTRAVAAFEALRPRLAASDAWSTLSQELSEEYPDLSF
                     FYRPQPTWIQEGASSRSANYTQRVMALGEDQVSTPIVETSGVYVVRVLEERRQGTPPF
                     EQVEDRVREDYIGDQVEEEFTERFSAWKAEARKHDTIQSFAAAVGREDQLTTAVETNV
                     TVIPGIGQIASTDRDYLLRDLDPGELSDVMQTTDGMVVVQIVDPKPAYDPELAEVREK
                     VEAALRRERAVALARAAAEQALNALEAGAEFDAALADAPVAPFRTDPLTRTQPVRNLG
                     APLIGLTQATLGVEEGSTGMTPYGYSEDSPVGFAVWQVADLEQPTVEEFDAERAEFER
                     DYLQVQRLTILREWQADLRRQAGFELLDQEGNPVRVAEGGATAAATAEPGAEAEEAAE
                     "
     gene            complement(2635..2847)
                     /locus_tag="PLS90_15240"
     CDS             complement(2635..2847)
                     /locus_tag="PLS90_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03801.1"
                     /translation="MVRRWRALPGAPGFVVAIIPAGAALEGAPGRDGWRAAVRCLPRI
                     ALVYLVLISVMAAWTARRGASGHFGP"
     gene            complement(2851..4245)
                     /gene="rlmD"
                     /locus_tag="PLS90_15245"
     CDS             complement(2851..4245)
                     /gene="rlmD"
                     /locus_tag="PLS90_15245"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPK03802.1"
                     /translation="MSEIRKNDLVTVEIQDLAYGGLGVARHNGFVVFVPNALPGERVA
                     ARIVKKKANHAQAVLERIEQASPHRIANPPCPVYDRCGGCTWQNLPHEEQTRWKEKQV
                     VDTLRHLGRQEAFETRPILRSPLAFRYRNKMEYSWGMEPSERHGAPQLVLGFHKPGRF
                     DRILPVPACLLQPEALDEMHATLLQWAREQGFGAHDPRRHEGFLRHAVMRYSFTTGEN
                     ILLLLTHEGDLTGKEQLVERLRERVGGFKAFVWGINRGVADVARIEEERWVWGNPILE
                     EEVNGLRFEISPQSFFQTNTAGAALLYAKVAELAELAADSRLLDAYCGTGAIGLHCAR
                     QAGRVVGVEMVTEAIWNARRNAERNGIDNTVFLAAPMPRGLELARCAAGGAFSHVVID
                     PPRGGMDKRSLAGLIDLQAPTFLYVSCNPATLARDIVTICEGGYVIDVVQPVDMFPQT
                     YHIETIVRLRRRDG"
     gene            4623..>4761
                     /locus_tag="PLS90_15250"
     CDS             4623..>4761
                     /locus_tag="PLS90_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HPK03803.1"
                     /translation="MTSTPQPRPANRLIHETSPYLLQHAHNPVDWFPWGEEAFAAARA
                     RH"
BASE COUNT          795 a         1778 c         1398 g          790 t
ORIGIN      
        1 cgctcaacgc tgacaacggg ggcacgggat ccagtcgcac ggcgcgtgaa ccgctcgagc
       61 gcagtcatct tacacgaccg ctccgacccg ctccaagacg aatccccgcc ccggcagaac
      121 gttcatccga atcactatcg gtaccggtat cgccggggcc gccaatcgaa agcaggcaac
      181 cccgcgccag cgggctgcct gtcatcggtt tagtccgtgg ggcgaacggc cggaataaag
      241 cgcggggtga cggctgcagg ccggaaccct gtgtcgccac gcccaacgcc ccaaacccct
      301 gcaaggggcg gccggagctc gtctgaattg cggggaatga taagcacgac agtggcggga
      361 aaccggggaa accagatcca aacagtcccg caggaacggc agaactctac cgcttcaaat
      421 tcatttcgga cttgtttcga atttcaaaat tcgcattccg agtttcattc ctctactccg
      481 ccgcttcctc cgcctcggcg ccgggttccg ccgtcgccgc cgccgtcgcc cccccttccg
      541 ccacccgcac cgggttcccc tcctgatcga gcaactcaaa gcccgcctga cgccgcagat
      601 cggcctgcca ctcgcgcagg atggtcagcc gctgcacttg caggtaatcg cgctcgaatt
      661 ccgcccgctc cgcgtcgaac tcctcgaccg tcggttgctc cagatcggcg acctgccaga
      721 cggcaaatcc cacgggcgaa tcctcgctgt agccgtaagg cgtcatgccg gtcgagccct
      781 cctcgacgcc gagcgtcgcc tgggtcaggc cgatcaacgg cgcgcccagg ttgcgcaccg
      841 gttgcgttcg cgtcagcggg tcggtgcgga atggcgcgac gggggcatcg gccagcgccg
      901 catcgaactc cgcgcccgct tccagggcat tgagcgcctg ctcggcggcg gcgcgcgcca
      961 gcgcgaccgc ccgctcgcgc cgcagcgccg cttcgacctt ctcgcgcact tcggccagct
     1021 ccggatcata cgccggcttc ggatcgacga tctgcaccac gaccatcccg tcggtcgtct
     1081 gcatcacgtc gctcaactcg cccggatcca gatcgcgcag caggtagtcg cggtcggtcg
     1141 aggcgatctg gccgatcccc gggatcacgg tcacgttggt ttcgaccgcc gtcgtgagtt
     1201 gatcctcgcg ccccaccgcc gcggcgaaac tctggatcgt gtcgtgcttc cgcgcctccg
     1261 ccttccacgc gctgaacctc tccgtgaact cctcctccac ctggtcgccg atgtaatcct
     1321 cccgcacccg atcctccact tgctcgaacg gcggcgtccc ctgccgccgc tcctccagga
     1381 cgcgaacgac atagacgccg ctcgtctcaa cgatcggcgt cgacacctgg tcctcaccga
     1441 gcgccatcac gcgctgggtg tagttggcgc tccggctcga cgccccttcc tggatccagg
     1501 tcggctgcgg ccggtagaag aagctcaggt cgggatactc ctcgctcagt tcctgcgaca
     1561 gcgtcgacca cgcatcggag gcggccagcc gcggccgcag cgcctcaaac gcggccacgg
     1621 cccgcgtcgt cgtgtcgtcc tccagcagcg gcacatacat ctcctccacc agccgcgccc
     1681 ccggttcaaa atagtccacc tggtgctgct cgtaatacac gtgcaggctg gcgctggtcg
     1741 gttcgagggc gtcgcgcaga tcctcgcgcg tcagcttcac gtaggcgtag cgccgcgtct
     1801 ccgggacgcg aaactcgtcg atgtgctcgg ccaggtagtc ctgcaactcc tgttcggtca
     1861 ccacgacctg gtcgacgaac tcggcggtcg ggtaggccgc catctggaac gtgatctttt
     1921 cgttgaacag cagatactcc tgccacagct cgaacagcga cgcgtgcgcc agcatcgtcc
     1981 gcagcgcgcg gacgcggctc tcaacctgat cctcgcggat catctcgacg aattgatgct
     2041 tgtccatccc catctgcatc gccgcggctt cgagccgttc ctcggtcatg tagggctgct
     2101 ggcgcagcat ctccaccacg tcccgggtcg ctacgtccac gcccatctcg tccgcatggc
     2161 gctggatcag ccgcttgtcg gccgctgcgt aggccagatc tctgtcggtc accaaccggt
     2221 tctcgatccc cgggtttgtc tggagcagca tctgcatctg cggcgggagc tggctcgcct
     2281 tctccatcag cacgtcctgg cgcagccggg ccgcctcgcg cacgctgatc gtctcccaac
     2341 tctccagcgg gttgatgctg cccgtgcgca gacgcgccac gtcgtagtcg gcattctgca
     2401 tccgacccgc gtcgggccgg aacccgtaga agaagatgaa gctgatgatg atgatcgcga
     2461 aaccgatgta gatcggccac ttcagcttct tggtatcgcg catatcctgc aatttgaaca
     2521 tgtaagcccg gttccttgcg ttggggaggt tgacggatgg cggccgccgc caaaactcgg
     2581 cggaaacggc aaaatagact attcttcaag cccgccaaac caagtcaaca gatttcatgg
     2641 accaaaatgc cccgatgccc cccgccgcgc cgtccacgcc gccatcacgc taatcaaaac
     2701 cagataaacc agcgcgatcc ggggcaggca gcggaccgcc gcgcgccagc cgtcgcgccc
     2761 cggcgcgcct tccagcgccg cgcccgccgg aattatcgcc acgacaaagc cgggcgcgcc
     2821 cggcaacgcg cgccaccggc ggaccatcgc tcagccgtcc cgccggcgca gccggacgat
     2881 cgtctcgata tggtatgtct gcggaaacat gtcgaccggc tggaccacat cgatcacgta
     2941 gcccccctcg cagatcgtga cgatgtcgcg cgcgagcgtc gccggattgc acgacacata
     3001 cagaaacgtc ggcgcctgca ggtcaatcag cccggccagc gagcgcttgt ccatcccgcc
     3061 gcgcggcgga tcgatgacga cgtgcgaaaa cgcgccgccg gccgcgcagc gcgccagctc
     3121 cagtccgcgc ggcatcggcg ccgccagaaa caccgtgttg tcgatcccgt tgcgctcggc
     3181 attgcgccgc gcgttccaga tcgcttcggt gaccatctcg acgccgacga cccgccccgc
     3241 ctggcgcgcg caatgcaggc cgatcgcccc cgtgccgcaa taggcgtcga gcagacgcga
     3301 gtccgccgcc agctcggcca gctcggccac cttcgcatag agcagcgccg cgccggcggt
     3361 gttggtctga aaaaacgact gcggcgagat ctcaaagcgc aggccgttga cctcttcctc
     3421 caggatcggg ttgccccaga cccagcgctc ctcctcgatc cgcgcgacgt cggccacccc
     3481 ccggttgatc ccccagacga acgccttgaa cccgccgacg cgctcgcgca accgctccac
     3541 cagttgctcc ttgcccgtca ggtccccttc gtgcgtcaac agcagcagaa tgttctcgcc
     3601 ggtggtgaag gagtagcgca tcacggcgtg gcgcaggaaa ccctcgtgcc gccgcgggtc
     3661 atgggcgccg aaaccctgct cgcgcgccca ttgcagcagc gtcgcatgca tctcgtcgag
     3721 cgcttcgggc tgcagcaggc acgccggcac cggcaggatc cggtcgaagc ggcccggctt
     3781 gtgaaagcca agaaccagtt gcggcgcgcc gtggcgctcg ctcggctcca tcccccacga
     3841 gtattccatc ttgttgcggt agcggaaggc cagcggcgaa cgaaggatcg gccgcgtctc
     3901 gaacgcctcc tgccggccca ggtggcgcaa cgtgtcgacc acctgctttt ccttccagcg
     3961 cgtctgctcc tcatggggca ggttctgcca ggtgcagccg ccgcaacggt cgtagacggg
     4021 gcagggggga tttgcaatcc ggtgcggcga ggcctgctcg atccgctcga gaaccgcctg
     4081 cgcgtggttc gccttcttct tgacgatccg cgccgcgacg cgctcgcccg gcagcgcgtt
     4141 gggcacgaac acgacgaagc cgttatggcg cgccacgccc agcccgccgt aggcgaggtc
     4201 ctgaatctcg accgtcacca ggtcattctt gcgtatttcg gacatgggcg gaaacgccgg
     4261 cctcccgccc cgcgcctccc gccgcgcagg acaatgatag ccccatgata ccgggcctcg
     4321 cgcacgatgc aaagggggat tggcgaccgc aaccccttcg cgaccgacgg tgtcacaagg
     4381 gatcgtcggc ttctttcacc tccatcagcc ccgcccaccc aaccggttca tcgccccttt
     4441 ggcctttccc ggaaaacctg ataaactgat ggaagctccg cgttgcaccg ggctcatgcc
     4501 gaggacgatg ccgaccgcac gcctccccgc cggcttcccg ccggccgctc gcaacgcttg
     4561 cgccggactc ccccctgctc cgacacgacc gcggcccctt ccgatcccca ggagaaacac
     4621 cgatgacctc caccccccag ccccgacccg ccaaccgcct gatccacgag accagtccct
     4681 atctgctgca gcacgcgcat aatcccgtgg actggtttcc gtggggcgag gaggccttcg
     4741 cggccgcgcg cgcgcgccac t
//
LOCUS       DAOPCO010000306         4750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14988_length_4750_cov_5.070501, whole genome shotgun sequence.
ACCESSION   DAOPCO010000306 DAOPCO010000000
VERSION     DAOPCO010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14988_length_4750_cov_5.070501"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..301)
                     /locus_tag="PLS90_15255"
     CDS             complement(50..301)
                     /locus_tag="PLS90_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPK03804.1"
                     /translation="MFGIGQTELIVLLVIVLLIFGPKNLPRLAQSIGRSARELKNGLN
                     GITEDLKDSAHEDRKEASAQPAVRSPEGTRVERNEGEKS"
     gene            complement(452..802)
                     /locus_tag="PLS90_15260"
     CDS             complement(452..802)
                     /locus_tag="PLS90_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03805.1"
                     /translation="MNRRRFVQLSLLLSLPSLAALPSLSPAATTTAATRSVYRLSVRG
                     RRGSRAAKSHCNNKLFASAEAADRNRAHRGDHARIVSFQMSEEDFSTLFSHSEVADLR
                     RLKRSSAGLWRDYR"
     gene            1286..2284
                     /locus_tag="PLS90_15265"
     CDS             1286..2284
                     /locus_tag="PLS90_15265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPK03806.1"
                     /translation="MTDRPWKLGIDLGGTKILAAVIGPDNAILSRAKKKTKVELGAEA
                     VLERIAQTAEEALQLASGSAEAIACAGIGAPGPLDPETGVVLEAPNLQWKEVALTAWL
                     SERLGIPSYLSNDVNAGTWGEYVLGAGQGSRSCIGVFVGTGIGGGLVINGQLYDGASH
                     IAGEIGHMCLDPNGPVCGCGRRGCLEAFASRTAMTRDLLNESKKRDREIPEVIEKGQL
                     IRSKEFRKKLSAGDPSYVRVFEGATECLGRGLASVTNLLNPDCIILGGGVVEALGSAY
                     VDRVREVLAAHAFPTAVGALRLVPAALGDDAGVLGAALLAQMQHERRPRREPASVS"
     gene            2317..3630
                     /locus_tag="PLS90_15270"
     CDS             2317..3630
                     /locus_tag="PLS90_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03807.1"
                     /translation="MRSTGLGLKSLVRHLTLIAAGVGFAMAAGCGANNESVAIEEIRE
                     LPAAERWGTAAASDAERFGFAPSAAIAPTPAAVTFSYEAPEGWTELPPAEFRNPNFTV
                     TGHPETEIYVTRLEGTGGGLAANINRWRGQFGLAPLTEAELAGLPKIEAAGGEGVFVS
                     FTGEFRGMGGASRGEAMLHGVVVERPGEAIFIKMTGPPDVVQSQAEAFKAFAASLKPV
                     EGTMAAGEATSGALTMQLPAGHPPMDAMSTLDGALPAGHPDVGQSLPAGHPDVGAAMP
                     GMVMTDMSTASGQLQWRKPEGWTEAPARAMREVTFHAGADGEVECYVSILGGAAGDAE
                     ANINRWRGQMGQSPLTAAEIGEFKRIAVLGQDAPLVEIEGSYRGMGGEALEEALMLGS
                     MAALEGQTVFVKMVGPRSAVAAQREAFIQFCRSLRPPEAENQAPE"
     gene            3865..>4750
                     /locus_tag="PLS90_15275"
     CDS             3865..>4750
                     /locus_tag="PLS90_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03808.1"
                     /translation="MKWYVKGPLEVLSSFGLALILLGFLFLLTLLGTLEQANLGLYEV
                     QRKYFTSFFLVHELGPLKVPLPGVYLLLILLAINLTLGVVYRTRPRLDRFGIYVMHGG
                     MLFMLLAGLVTHHFATEGQMTLAPGESSNRFQSYHEWELLIRGPIGPGEAAREYAIPG
                     EDFLRLGRTESRTFVAETLPFDVTVSDVARNAVPQRLAPEQRNASGMGVDGFYLAVLA
                     PEKENERNLPGFHVTLTDKRGGERTEEIVWGGSTLPLEIARLARLSYLNLGRNQMLEI
                     PPGVWRLSGLERLVIDHTQ"
BASE COUNT          787 a         1300 c         1853 g          810 t
ORIGIN      
        1 ggggtcagac aagagaagcg gggccgggca gcggggcgcg aagcgccgtt caggactttt
       61 cgccctcgtt gcgttcgacg cgcgtccctt ccgggctgcg cacggcgggc tgtgcggagg
      121 cttccttgcg atcctcgtgg gcgctgtctt tcaggtcctc ggtgatgccg ttcaagccat
      181 tcttcagttc acgggccgag cgaccgatcg attgcgccag gcgcgggagg tttttgggcc
      241 cgaaaatcag cagaacgatg acgagcaaga cgataagttc agtctgaccg atgccgaaca
      301 tgatgcgaat ccttttccga tggaggctgc gcgctgtggg gcgacgccgg ttgcgtcagg
      361 cggccggcca tcagggaaca tgctagcacg cgggttgaag cagggaaaag cggaatcaga
      421 gccgggggcg ttagccggag ggtggatggg atcaacggta atcgcgccaa agaccggccg
      481 aggaacgctt cagccggcgc aaatcggcga cctcggaatg actgaagagg gtggagaaat
      541 cctcttcgct catttgaaaa ctgacgatgc gggcatggtc gccgcgatgg gcgcggttgc
      601 gatcggccgc ctcggcgcta gcgaacagct tgttgttgca atggcttttc gccgcgcgcg
      661 agccgcggcg tccgcgaacc gacaggcggt agacggagcg ggtggcggcg gtggtggtcg
      721 ccgcggggga gaggctgggc agggccgcca gactggggag ggagaggagg aggcttaact
      781 gaacaaaacg gcgacgattc atgttgtgca gccaaaggtg agagcggggc ggggggaagc
      841 gccgcatttc cccgtcggtt cggccgttgc ggccggccca gactttgtcg cgcgacttac
      901 ggggcggaac cgggcgggac ggcccgccct ccgcgaaccg cgctgcaaac gcggcaaatc
      961 ggtcggcttg ggctcccctg acccacgggc cagagtgaac ttacacgttc caatcagcag
     1021 ggcttgcagt ctccgccggt cgaactcgcg ctctttccga cttgagagga tgatatccgc
     1081 ctcaacaatc gtggtcaagc gtggatggtg cggccgggat tccctctgcg ggcttgtctc
     1141 cgccatctgc ttcgcggact ctcgggtttg gcctaggctg cggtgcgatt gaggactata
     1201 atgaggcgcg catggttgcc cggggccggt gctgggggtt ttccggttca cgccgcggat
     1261 accattttgc ccatggagag agttcatgac cgaccgccca tggaagctcg ggatcgatct
     1321 gggcgggacg aagatcctgg cggccgtgat cggcccggac aacgcgatcc tcagccgcgc
     1381 caagaagaag accaaggtgg agctgggggc ggaggccgtg ctggagcgaa ttgcacagac
     1441 ggcggaagag gcgctccagc ttgcgtcggg ctcggcggag gcgatcgcct gcgccgggat
     1501 cggggcgccc ggcccgctgg atccggaaac aggggtcgtt ctggaggcgc ccaatctcca
     1561 gtggaaggaa gtcgcgctga cggcctggct gagcgagcgg ctcgggatcc cgagctacct
     1621 gtcaaacgat gtcaacgccg gcacgtgggg ggaatatgtg ctcggcgcgg ggcagggctc
     1681 gcgctcgtgc atcggggtat tcgtcgggac gggaatcggc ggggggttgg tgatcaacgg
     1741 ccagctttat gacggcgcca gtcacatcgc gggggagatc ggccatatgt gcctggaccc
     1801 gaacggcccg gtgtgcgggt gcgggcggcg gggctgcctg gaggccttcg cgtcgcggac
     1861 ggcgatgacg cgcgacctgt tgaatgagag caagaaacgg gatcgtgaga tcccggaagt
     1921 gatcgagaag gggcagttga tccgctcgaa ggaattccgc aagaagttga gcgccgggga
     1981 tccgtcgtac gtccgggtgt ttgaaggggc gaccgaatgc ctgggccggg gactggcgtc
     2041 ggtgacgaat ctgctgaacc cggactgcat catcctgggc gggggcgtcg tggaggcgct
     2101 gggcagcgcg tatgtggatc gggtgcgcga ggtgctcgcc gcgcatgcgt tcccgacggc
     2161 cgtgggggcg ctacggctgg ttccggcggc gctgggggac gatgccggcg tgctgggagc
     2221 cgcgctgctg gcgcagatgc agcatgagag gcggccgcgg cgcgagccgg cctcggtcag
     2281 ttgatcccac attccataga acaggaaatg gcggcgatgc gatcgaccgg actcggcctg
     2341 aagagccttg tgaggcattt gacgctgatt gcggcgggcg tgggtttcgc gatggcggcc
     2401 ggttgcgggg cgaacaacga gtcggtcgcg atcgaggaga tccgcgagtt gccggcggcg
     2461 gagcgctggg ggacggcggc ggcgagcgat gcggaacggt tcggattcgc cccgtcggcg
     2521 gcgatcgcgc cgacgcccgc ggccgtgacg ttcagctatg aagcgcccga ggggtggacg
     2581 gagcttccgc ccgccgagtt ccgtaatccg aatttcacgg tgacggggca tccggagacc
     2641 gaaatctatg tgacgcggct ggaagggacg ggcggcggtc tggcggcgaa catcaatcgc
     2701 tggcgggggc agttcggcct ggcaccgctc accgaggccg agctggcggg gctgccgaag
     2761 atcgaggcgg ccggtggcga gggggtgttc gtgagtttca ctggcgagtt tcgcgggatg
     2821 ggcggcgcct cgcggggcga ggcgatgctg cacggggtgg tggttgagcg gccgggggaa
     2881 gcgatattca tcaagatgac gggtccgccc gacgtcgtgc agtcgcaggc cgaggcgttc
     2941 aaggcgttcg ccgcctcgct gaaaccggtt gaaggaacga tggcggcggg ggaggcaacg
     3001 agcggcgcat tgaccatgca gcttcccgcc gggcatccgc cgatggacgc gatgagcacg
     3061 ctcgacggcg cgctgccggc gggtcatccc gacgtggggc agtcgctgcc ggcgggccac
     3121 cccgacgtgg gggcggccat gccggggatg gtgatgacgg acatgagcac cgcctcgggg
     3181 cagctccagt ggcggaagcc ggaggggtgg acggaagcgc cggcgcgggc gatgcgcgag
     3241 gtgacgtttc acgcgggagc ggatggcgag gttgagtgtt atgtctcgat actcgggggc
     3301 gcggcggggg acgcggaggc caacatcaat cgctggcgcg gccagatggg gcagtcgccg
     3361 ctgacggcgg cggagatcgg ggaattcaag cggatcgcgg tgctgggcca ggacgcgccg
     3421 ctggtggaga tcgaggggag ctatcggggg atgggaggcg aagcgctgga ggaggcgctg
     3481 atgctgggtt cgatggccgc gctggagggg cagacggtgt ttgtgaagat ggtgggcccg
     3541 cggtcggcgg tcgccgcgca acgcgaggcg ttcatccagt tctgccgttc gctccgcccg
     3601 ccggaagcgg aaaatcaggc tcccgaataa ccgtgaaagg atgaaatgat tcgccggcct
     3661 tggtctggct gagatttgcc ccctgcgggg acggggggtt ttcgcgacag catccccagg
     3721 ttccgctgcg ctccaacccg gggctgttca actgccgccc cgctgggggc ggcttatgcg
     3781 gagggctttt ggggacaaaa ttgaggcaag ccttcggggg cgatcacgag gacgagggac
     3841 ggattttgga gtgaggacga aagcatgaag tggtatgtca agggcccgct cgaggtgttg
     3901 agttcctttg gcctggccct gattctgctg ggatttttgt ttctgttgac gctgctgggc
     3961 acgctggaac aggccaatct cggactgtat gaagttcagc ggaagtattt cacgtcgttc
     4021 tttctggtgc atgaactggg accgctgaag gttccgcttc cgggcgttta tctgctgctg
     4081 atcctgctgg cgatcaatct cacgctgggc gtggtgtatc ggacgcggcc gcggctggac
     4141 cggttcggga tttacgtgat gcacggcggg atgctgttca tgctgctggc ggggctggtg
     4201 acgcaccatt tcgcgacgga aggccagatg acgctggcgc cgggcgaaag ttccaaccgg
     4261 tttcagagct atcacgaatg ggagttgctg attcgcgggc cgatcgggcc gggcgaggcg
     4321 gcgcgggagt atgccatccc gggggaggat tttctgcgcc tggggcgaac cgagagcaga
     4381 acgtttgtcg ccgagacgct gccattcgat gtcacggtga gcgacgtcgc ccgcaacgcg
     4441 gtgccgcagc ggctggcgcc cgagcagagg aacgcatcgg gcatgggcgt cgacggcttt
     4501 tatctggccg tgctcgcgcc ggagaaggag aacgagcgga acctgcccgg gtttcatgtc
     4561 accctgaccg acaagcgcgg gggggagcgg accgaggaga tcgtgtgggg cgggtcgacg
     4621 ctgccgctgg agatcgcgcg gctggcccgc ctcagctatc tcaacttggg gcgtaaccag
     4681 atgcttgaga tcccgccagg cgtgtggcgc ctgagcggcc tggagcgcct tgtcattgac
     4741 cacacccagg
//
LOCUS       DAOPCO010000307         4750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_14990_length_4750_cov_4.706283, whole genome shotgun sequence.
ACCESSION   DAOPCO010000307 DAOPCO010000000
VERSION     DAOPCO010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14990_length_4750_cov_4.706283"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..1644
                     /locus_tag="PLS90_15280"
     CDS             355..1644
                     /locus_tag="PLS90_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03809.1"
                     /translation="MSDDARNSPTRGTRYDMDPDAMAGDQGSDATDPRLSDEEREQSR
                     QVRELGAHDAPTPTRAEARDAGESIRKHLSEAVTAFEANDAEAAANHLTEAAVLLRNL
                     AGRMDRHVPRMRRGGPDRPGAQPGLVTGAVPGAPGILPAGQPVPREDRPTTGDMAPPA
                     DGGMDEARERIEAELTDSQRNLRDSIRRVEELATRIEKNEVDNADDLRSVISDLHRSL
                     AEYHYDVAHRILSRVDRAAWRGPGMRRSAETQGLLTPAETDRMEAEEDETVLTQEDAA
                     EPEELDADALAADQATTATLNRRQRAALALNLAESAASFRAWSELNQKDFDDETEQVV
                     ATIENWGVKVREGSDWRAAELRQALDTFGARLDDHGFTVRNAYGIRDPRPVTGGGEGS
                     LPAERPRDRRDGRDEAKNDRPQSGVPAMDPEPNAATD"
     gene            complement(1890..2801)
                     /locus_tag="PLS90_15285"
     CDS             complement(1890..2801)
                     /locus_tag="PLS90_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016483084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK03810.1"
                     /translation="MSLPKRTFGRTGLEVTQLGYGAMEVRGERIWGGRPCNDEQARTI
                     LNAVLDAGINFIDTANDYGKSELYIGRHLAQRQGEFFIATKCGCTMTYAGDHDDTPHF
                     WDRDHIRRNIADSLLKMQMSRVDLLQLHNPSVELAEKFKLVDTLQELQREGAVRFIGC
                     SSTSPDLATYIEWGVFDAFQIPYSALERRHENLITRAAQAGAGVIIRGGVARGEPGQG
                     LGAGDRWRKFEEAKLDELLAPGESRTAFLLRFTLSHPHCHTTIVGTMKPEHLAENVRI
                     AQVGALPADVYEEAKRRLDAVGESPEA"
     gene            complement(2979..3587)
                     /locus_tag="PLS90_15290"
     CDS             complement(2979..3587)
                     /locus_tag="PLS90_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03811.1"
                     /translation="MRLLGAPPFYLAALVLFLGIRVTFQSQTYLHDYITEVKTLPVLS
                     DLILDNLPYWDIGFVYDVFSLLSLLIFAVYVFHRRAFAEVPYFLLLCGLFHLVRGVFI
                     VLTPFGNPPMFDGTEGPFNGFSNFELGVYPSGHTGISILYCLLVSSRGYRAALGICVA
                     VVVASLFLARGHYSIDVLSGIFFAYAIKAFGDKHLRDRVVLK"
     gene            complement(3604..4380)
                     /locus_tag="PLS90_15295"
     CDS             complement(3604..4380)
                     /locus_tag="PLS90_15295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPK03812.1"
                     /translation="MIVIAYNEERYLPALLESLAKQTWREFELIVVDSHSRDRTREAA
                     RARAGCFGDFHLVEMDRPRGPARARNVGAARATRERLLFLDADSVLAPTFLERAMAAL
                     AERPADVATCFIRINEGGLVSRAGALLFNAGLWALRPVYPTAFGACLFSTRELHGALG
                     GFDEQLALCEDCEYVKRALRRPGARFRILPLAFGTSDRRSRAEGGARILLKYLGVHAY
                     RLLTRKELAKGRVVYEYGEYDQQPGGQEAGRIWGGSPGAR"
BASE COUNT          868 a         1837 c         1310 g          735 t
ORIGIN      
        1 aaacccgcat ctcccttcat ccccccggcc ccatccctcg cccccctatt cgaccgtgta
       61 aagatcgcct atcccctgcc ttcaatccgc ctttccttgt ccggccgcca gccccctcac
      121 gctcctcatt tgactggatt tgcccctacg cccgcgtccc ccgcccgcgc cctccggccc
      181 gtggcatgga acgtgctcca gcctgattcg ttcggacctg ttccatccgg ttagctctcc
      241 aaacctcgaa cttgaacgac caaccacagg agtatgacga tgcgaccgct tgcttccctt
      301 cttctcgtgc tcgtgatcgg agccggcccg ctcacccttc cggctcaacc cgtcatgtcc
      361 gacgacgcca ggaattcccc cacccgcggg acgcgttacg acatggatcc cgacgccatg
      421 gccggcgacc agggttcgga cgcgaccgac ccccgcctca gcgacgagga acgcgaacag
      481 agccgccagg tccgcgagct cggcgcccac gacgccccca cccccacccg ggccgaagcc
      541 cgcgacgccg gcgaaagtat ccgcaaacac ctcagcgagg ccgtgaccgc gttcgaagcg
      601 aacgacgccg aagccgccgc caatcacctg accgaggccg ccgtcctgct gcgcaacctg
      661 gccggacgca tggatcgcca cgtcccgcgc atgcgccgcg gaggccccga tcgccccggt
      721 gcgcaacccg gcctcgtcac cggcgctgtc ccgggcgcac ctggcattct ccccgccggt
      781 caacccgtcc cgcgcgaaga tcgccccacc accggcgaca tggccccccc ggccgacggc
      841 gggatggacg aggcccgcga acggattgag gccgaactaa ccgactccca acgcaacctg
      901 cgcgattcga tccgccgggt cgaagagctg gccacccgca tcgaaaaaaa tgaggtcgac
      961 aacgccgacg acttgcgctc ggtcattagc gacctccacc gctcgctggc cgaataccat
     1021 tacgacgtcg cccaccgcat cctctcgcgt gtcgaccgcg ccgcgtggcg cggaccgggc
     1081 atgcgccggt cggccgagac ccagggcctc ctgacccctg cggagaccga tcgaatggag
     1141 gccgaggagg acgagaccgt tctgactcag gaagacgcgg cggaaccgga ggagctggat
     1201 gccgacgcgc tggcggcgga tcaggccacg actgctacgt tgaaccggcg tcagcgcgcg
     1261 gcgctcgccc tgaacctcgc cgaatccgcg gccagcttcc gggcctggtc cgagctgaat
     1321 cagaaggatt tcgacgacga aaccgagcag gtcgtcgcaa ccattgaaaa ctggggcgtc
     1381 aaagtcaggg aagggtccga ctggcgcgcg gccgaactcc gccaggccct cgacaccttc
     1441 ggcgcgcgcc tcgacgatca cggcttcacc gtccgcaacg cctacggcat ccgcgatcct
     1501 cggccggtga ccggcggcgg cgaaggctcg ctccccgccg aacgtccccg cgatcggcgt
     1561 gacggccgcg acgaagcaaa gaacgaccgc ccccagtccg gcgtccccgc catggaccct
     1621 gaacccaacg ccgcaaccga ctgataatcg gtatcgcgat cgctatcgca ctcgggcgcc
     1681 atcctggaaa ttggattccc cgcgcctccg cgtgggatcg ttctccggag cgataccctc
     1741 acgcccgcgc cgtcaatgac ctactggggg acgcccgagg atgaacccgc ggcacaacct
     1801 tgttcgttac atgacggatg gcttccgcgg atcagtccgc gaagcggacg atcagaataa
     1861 aatgcagtcg ggcatcgcgc ctcgcttcct cacgcctccg gcgattcccc gaccgcatcc
     1921 agacgccgtt tggcttcctc atacacgtcg gccggcagcg cgcccacctg cgcgatccgc
     1981 acattctccg ccaggtgctc cggcttcatc gtcccgacga tcgtcgtgtg gcaatgcgga
     2041 tgactcagtg tgaaccgcag caggaacgcc gtccgcgatt cccccggcgc cagcagctca
     2101 tccagtttcg cctcctcgaa tttgcgccac cggtcgccgg cccccagccc ctgacccggt
     2161 tcgccgcgcg ccacgccccc ccggatgatc acgcccgccc ccgcctgcgc cgcccgcgtg
     2221 atcaggttct catgccgccg ctccagcgcc gaatacggaa tctgaaacgc atcgaacacg
     2281 ccccattcga tgtaggtcgc caggtccggg ctcgtcgacg aacacccgat gaagcgcacc
     2341 gccccctcgc gctgaagttc ctgcagcgta tcgaccagct tgaacttctc ggccagctcc
     2401 accgacggat tgtgcagttg cagcaggtcg acccgcgaca tctgcatctt cagcagcgaa
     2461 tcggcgatgt tgcgccggat gtgatcccgg tcccagaaat gcggcgtatc gtcatgatcg
     2521 cccgcatacg tcatcgtgca gccgcacttg gtcgcaatga agaattcccc ctgacgctgc
     2581 gccagatgcc gtccgatgta gagctcgctc ttgccgtagt cgttcgccgt gtcgatgaaa
     2641 ttgatccccg cgtcgagcac cgcgttcagg atcgtccggg cctgctcgtc attgcacggc
     2701 cggccccccc agatccgctc gccgcgcact tccatcgcgc cgtagcccag ctgcgtgacc
     2761 tccagaccgg tcctgccgaa tgtcctcttg gggagactca tgggtttctc ctctcgcgct
     2821 ttcaagtgat atccgtgcgg atcatcatcc taccacgccc cctccgccaa cgccaccccg
     2881 cgagcgcggg gtgtttctcc aaacttcggc tctcgtctct cgtctctcgt ccctcgtctc
     2941 tcgtcttcgg ctctcggatc cccgccgcgt tcgccccctc acttcaacac gacccggtcg
     3001 cgcaggtgct tgtcgccgaa cgccttgatc gcataggcga agaatatccc cgacagcaca
     3061 tcgatgctgt aatgcccccg cgccagaaac agcgacgcca ccaccaccgc cacgcagatc
     3121 cccagcgctg cccggtagcc ccgactgctc accagcagac aatacagaat gctgatcccc
     3181 gtatgccccg aaggatagac ccccagctcg aagttcgaaa agccattgaa cggcccctcc
     3241 gtcccgtcga acatcggcgg attgccgaac ggcgtcaaca cgatgaacac cccgcggacc
     3301 agatgaaaca ggccgcacag caatagaaaa tacggaacct cggcgaacgc ccgccggtga
     3361 aacacataca cggcgaagat cagcagcgac agcagactga acacgtcata aacaaaaccg
     3421 atgtcccagt acggcagatt gtccaggatc agatcggaca ggaccggcag cgtcttgacc
     3481 tcggtgatgt agtcatgcag atacgtctgc gactgaaacg tcacccggat gccgagaaac
     3541 agcaccagcg ccgccagata gaacggcggc gcccccagca gccgcattat ctctctcagc
     3601 cggctaccga gcgccgggtg aaccgcccca aatccgaccc gcctcctggc cgccaggctg
     3661 ttggtcatac tctccgtact catacaccac ccttcccttc gcgagttcct tgcgcgtcag
     3721 cagtcggtag gcatgaaccc ccagatactt cagcaggatg cgcgcgcccc cctcggcccg
     3781 cgaccggcgg tccgacgtcc cgaacgcgag cggcaggatg cggaaccgcg cgcccggccg
     3841 ccgcagcgcc cgcttgacgt actcgcagtc ctcgcagagg gcaagctgtt catcgaatcc
     3901 ccccagcgcg ccatgcagct cgcgggtcga gaacaggcac gccccgaacg ccgtcggata
     3961 gaccggccgc agcgcccaca ggccggcatt gaacaagagc gcgcccgccc gcgaaaccag
     4021 ccccccctca ttgatccgga tgaaacacgt cgccacgtcc gcgggccgct cggccagagc
     4081 cgccatcgcc cgctccagaa acgtcggcgc aagcacgctg tcggcatcga gaaacagcag
     4141 ccgttcgcgc gtcgcgcgcg ccgcccccac gttgcgcgcc cgcgccgggc cgcgcggccg
     4201 atccatttca accaggtgaa aatctccaaa acacccggcg cgcgcccgcg ccgcctcgcg
     4261 cgtccgatcg cggctgtggc tgtcgaccac gatcagctcg aattcgcgcc aagtctgctt
     4321 cgccagcgac tccagcagcg ccggcagata ccgctcctcg ttgtaggcga tgacaatcac
     4381 gctgagcaag ctgtcggttc cgtgcccggg cgctcgatcc cgcccgcgat cccaaaccca
     4441 tcatatcatc agaacgcccc ggcccaggca atcgctttat cccccacctt ccgccgctcc
     4501 acgccccatg ggggaccagc ccccctgccc cgattggctc atccccccgg tcttccccgc
     4561 atcccggagc gatgccacac gtcgccccgg acgggcgccc tttccattag gccccggtga
     4621 atcgtaccgc agggatgccc tggagtttgg ccattcactc ggtcagctgg agggatgctt
     4681 cgaggcgcat cccgcaggga tgccggatcg tagccggtgg ttgagcgcag cgataccacc
     4741 ggtcatccgc
//
LOCUS       DAOPCO010000308         4744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15017_length_4744_cov_4.724888, whole genome shotgun sequence.
ACCESSION   DAOPCO010000308 DAOPCO010000000
VERSION     DAOPCO010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15017_length_4744_cov_4.724888"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..413
                     /locus_tag="PLS90_15300"
     CDS             54..413
                     /locus_tag="PLS90_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03813.1"
                     /translation="MPVVTRRITENTVWIEDGGTAIIAGLTDTRNRKGAERVPYLHRI
                     PILGRLFRADIWSDTERQVGVFITAHLMQEGPSTETVTPNTFWNKTTMGDYAPADEAF
                     VAEMQAALERYTGRSVQ"
     gene            410..793
                     /locus_tag="PLS90_15305"
     CDS             410..793
                     /locus_tag="PLS90_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03814.1"
                     /translation="MKIALQVTLLLALWMHLGCARYEIRPLPNESQTVLDADQTVQVL
                     LQVGLSDQVILKHGARVRETLARTGAVQIRNGRYTEAILAAHPPYIYMTCRDKGSMIY
                     PLEPVSRAVEPELSDARQALPVEVP"
     gene            complement(810..2219)
                     /locus_tag="PLS90_15310"
     CDS             complement(810..2219)
                     /locus_tag="PLS90_15310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03815.1"
                     /translation="MSVSSNPYLALLTRPIPFERLNTRRNRVALGRGLTLWLAFLWII
                     PYNHDPWRLTGLVIAGWMLILSILLLEGRKLLAGPGLWFCLVLTGWFVLATPADDVER
                     ARCFAQAVAAGLAVTCLPRAVAAAIPRRLLLSGSIALAISFVDGTLNQGLGFLQPWRP
                     IRYMEMENTLFRWTGVFQHPNIFALAIVVTVACAFACRWPADRRGPLLALMLGIVAGW
                     CMFMTYSRTGQVMLALTLLGGMALAWPRRSRRRPLAPLLALCLGVLVSFTLGAALHWD
                     KLARRYVAATTDMQPTHDWRLFGANDDPTLAPDQYARAIDPDAVDGPIAGTGRQAIAI
                     SRVDMARDALNLWQRRFWTGWGLGGYFRHGLSSYHHAHNLPLEIAVSGGLIGLLLFFL
                     PIALALLAGNSPLIRGFILVTLLSGFIDCYFVYRWPAVMLALFVGLALRGISPRLLRA
                     GSRLKRASVRTDPPLHIPD"
     gene            complement(2438..3895)
                     /locus_tag="PLS90_15315"
     CDS             complement(2438..3895)
                     /locus_tag="PLS90_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK03816.1"
                     /translation="MKLPLVRIHDLNLRPIPLLLPLALALCLAVAGCAEVESLRQRSA
                     TQESRIEALRQENEEFRANYYKIKEMYDAEVASSRKEIDRLTRELDEARNLKSQKEKE
                     LQDQLQSLQLEFEAHRREADDLKTYADTQITNLTRDVRTLTTERDAALQKVNQLTTQL
                     MDEQQINTRLTEELAALKVEAQSLSQQVATLQEELTIRGEELEGEKSARQAAEAQVAQ
                     VTTERDELTQKLTAAEEELTRANDSIDSMRASLEQVQQERDAARQELDAASQSAAARD
                     EELSTLRRDLETAQARITELEAAPAAAEETAKLRAEHEQEVAALRQQLEEAHAARPDL
                     STDPELQALADRVRGALADAQGAQVELDRKGLRIILAGSALFAPDSVVLSNESAPLLD
                     RVAEALSEVDGRRIRIEGHTDNTPVGDTLPFADNWGVGFARADRVREYLARRGVPAAR
                     MRAISAAEYEPRASNDTSEGRARNRRVEVVIAEPL"
     gene            complement(4040..>4744)
                     /locus_tag="PLS90_15320"
     CDS             complement(4040..>4744)
                     /locus_tag="PLS90_15320"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK03817.1"
                     /translation="RLGLRGWPSVAESIQVARDAELSEFTGGRIHVMHVSTRASVEII
                     RQARARGVRISCEVTPHHLALTVEACRTYSTNAKCNPPIGPESDRLALIEGLLDGTIE
                     VIATDHAPHTEMEKDLMFTDAPNGIIGMETAFGVLNTVLVRPGLMTLPDLISRMTANP
                     ARLLRIDKGTLSDGADADVALLDPEQVWTVDPERLHSKARNCPWNGQQLVGRPVATFV
                     GGELIYRDGRILDPAR"
BASE COUNT          790 a         1381 c         1796 g          777 t
ORIGIN      
        1 gctggtgatg ctggcgccgg cgatcgaagg gccggcggac tagtgggaac aatctgccgg
       61 tcgtgacgcg gcggatcacc gagaacaccg tgtggatcga agacgggggg acggcgatca
      121 tcgccgggct gaccgacacg cgcaatcgca agggggcgga gcgggtcccc tatttgcacc
      181 ggataccgat tctcgggcga ctgttccgcg cggacatctg gtcggatacc gagcgccagg
      241 tgggggtgtt tatcacggcg cacctgatgc aggaggggcc ttcgaccgaa accgtgacgc
      301 cgaacacgtt ctggaacaag accaccatgg gagactacgc gccggccgac gaggcgttcg
      361 tcgccgagat gcaagccgcg ctcgagcggt acaccgggag gtcggtccaa tgaaaatcgc
      421 gctgcaagtc accctgctgc tcgctttgtg gatgcacctg gggtgcgcgc gttacgagat
      481 ccggccgctg cccaatgaga gccagaccgt tctggacgcc gaccagacgg tgcaagttct
      541 gttgcaggtt gggttgagcg atcaggtaat tctgaagcac ggagcacggg tgcgcgaaac
      601 gctggcgcga accggagcgg tgcagatccg gaatggtcgg tatacggaag cgattctggc
      661 cgcgcatccg ccgtatattt acatgacctg ccgggacaag gggagcatga tctatccgct
      721 ggaaccggtt tcgcgggccg tggaaccgga gctgtcggac gcgcgacagg cgctgcccgt
      781 ggaggttcct taagaccggc tgatctggat caatccggaa tgtggagcgg tggatcggtg
      841 cgtacgctgg cgcgtttcag ccttgaaccg gcgcggagga gtcgcgggct gatgccgcgc
      901 agggcgaggc cgacaaacag ggcgagcatc accgcgggcc agcggtagac gaaatagcag
      961 tcaatgaagc ccgagagcaa cgtaacgagg atgaagccgc ggatcagcgg cgagttgccg
     1021 gcgagcaggg cgagcgcgat cgggaggaag aagaggagaa ggccgatcag accgcccgaa
     1081 acggcaatct cgagcggaag attgtgggcg tgatgatagc tggacaggcc gtggcggaag
     1141 tatccgccca gaccccagcc ggtccagaat cggcgctgcc acagattgag ggcatcgcgg
     1201 gccatatcga cgcggctgat ggcgatggcc tgtcgtccgg tgccggcgat ggggccgtcg
     1261 acggcatcgg gatcgatcgc gcgtgcgtac tggtcgggag ccagcgtggg atcgtcgttc
     1321 gcgccgaaca gccgccagtc gtgggtcggc tgcatgtcgg tggtggcggc gacgtagcgg
     1381 cgcgcgagtt tgtcccaatg gagcgcggcg cccagtgtga aactgacgag gacgccaagg
     1441 caaagggcga ggagcggggc cagcgggcgg cgccggctcc gccgcggcca ggccagagcc
     1501 atgccgccca ggagcgtaag cgcgagcatc acctggcccg tccggctata ggtcatgaac
     1561 atgcaccagc cggcgacgat gccgagcatg agcgccagca gggggccgcg gcgatcggcg
     1621 ggccagcggc aggcgaaggc gcaggcgacg gtgacgacaa tggcaagggc gaaaatgttg
     1681 gggtgctgaa aaacgccggt ccagcgaaag agggtgtttt ccatttccat atagcggatc
     1741 gggcgccagg gctggaggaa gccgaggccc tggttgagcg ttccgtcaac aaatgaaatg
     1801 gccagggcga tgctgcccga cagcaggagg cggcggggaa tcgccgccgc caccgcgcgc
     1861 ggcaggcagg tgacggccag cccggcggcg accgcctgcg cgaagcagcg cgcacgttcc
     1921 acgtcgtccg cgggcgtcgc gagcacgaac cagccggtca gcacgaggca gaaccagaga
     1981 cccggaccgg cgagcagctt gcgtccctcc aggagcagga tcgagagaat aagcatccag
     2041 ccggcgatga ccaggccggt cagccgccag ggatcgtggt tatacgggat gatccagagg
     2101 aaggcgagcc agagggtcag gccgcgaccc agtgcaacgc gattgcgccg ggtgttgagc
     2161 cgttcgaagg ggatgggccg ggtcagcagg gccaggtagg gattgctgga gacggacacc
     2221 cggacgcact cctccgagtg gtaggcgaag tccacgatcg ccgcgcagcg ccatggcagg
     2281 gcaattggac aggaccctta cttcctcgaa tatataccgg gtcgggcggc gggggcgcaa
     2341 tgcatttccg gtggcggatc ggcgtgagcg tgccggcatc cgcgttctca acgatcaaga
     2401 atcggcgagg cttcgatgag ccgtcacgcc ggatgtttta aagcggctcg gcgatgacga
     2461 cctcgacgcg gcggttgcgg gcgcgtcctt cgctcgtgtc gttgctggcg cgcggttcat
     2521 attcggcggc ggagatggcg cgcatgcggg cggccgggac gccgcggcgg gcgaggtatt
     2581 cgcggacgcg gtcggcgcgg gcgaagccga cgccccagtt gtcggcgaag gggagcgtat
     2641 cgccgacggg ggtattatcg gtgtggccct cgatgcggat gcggcggcca tcgacctcgc
     2701 tgagggcttc ggcgacgcgg tccagcagcg gggcgctctc gttggaaagg acgacggaat
     2761 cgggggcgaa cagggcgctg ccggcgagga tgatgcgcag gcccttgcgg tcgagttcga
     2821 cctgggcgcc ctgggcgtcg gccagcgcgc cgcggacgcg atcggcgagg gcctgcaact
     2881 cgggatccgt ggagagatcg ggccgggcgg cgtgggcctc ttcgagctgc tggcggagcg
     2941 cggcgacttc ctgctcgtgt tcggcgcgga gcttggcggt ttcctcggcc gcggcgggcg
     3001 cggcttccag ttcggtgatg cgcgcctggg cggtttccag atcacgccga agggtggaca
     3061 gctcctcgtc gcgggcggcg gccgactggc tggccgcgtc gagttcctgg cgcgcggcgt
     3121 cgcgctcctg ctgaacctgt tccaggctgg cgcgcatcga gtcgattgag tcgttggcgc
     3181 gcgtgagttc ctcttcggcc gcggtcagct tctgggttag ctcgtcgcgt tcggtggtca
     3241 cctgggcgac ctgagcctcg gcggcctggc gcgcggattt ttcgccctcc agttcctcgc
     3301 cgcggatggt caattcctcc tgcagcgtcg ccacctgctg gctgagggac tgcgcctcga
     3361 ccttgagggc ggccagttcc tcggtgagcc gcgtgttgat ctgctgttcg tccatcagtt
     3421 gggtcgtgag ctggttgacc ttctgcaggg cggcatcgcg ctcggtggtc agggtgcgga
     3481 cgtcgcgggt gaggttggta atctgcgtgt cggcgtaggt tttcaggtcg tcggcttcgc
     3541 ggcgatgcgc ctcaaactcg agctgcaggg actgaagctg gtcctgcagc tctttctcct
     3601 tctggctctt caggttgcgg gcctcgtcga gttcgcgggt caggcggtcg atttccttgc
     3661 gcgaggaggc gacctcggcg tcgtacattt ccttgatttt atagtagttg gccctgaatt
     3721 cctcattctc ctggcgaagc gcctcgatgc gcgattcctg ggtcgccgag cgttggcgca
     3781 gcgactccac ctcggcacag ccggcgaccg ccaggcacag cgcgagcgcc agcgggagga
     3841 ggagcgggat cggacgaagg ttcagatcgt ggatgcggac aagggggagc ttcatggggt
     3901 cgggtgcgga catccaggtt caagatcagc gggcgccagg gcccgtggcc tatccatcat
     3961 agcagaccag ccgcccggcg cgggagattg gcttctgggc gaagtatcgg gcgcgggcga
     4021 gccggaggtc gcggcgggtt taccgagccg ggtccaggat ccgtccatcg cggtagatca
     4081 gctcgccgcc gacaaacgtc gccaccggcc ggccgacaag ctgctgcccg ttccatgggc
     4141 aattgcgggc cttgctgtgg agccgttcgg gatcaaccgt ccaaacctgt tcggggtcca
     4201 ggagcgcgac atcggcgtcg gcgccgtcgc tcagcgtgcc cttgtcgatg cgcagcaggc
     4261 gggccgggtt ggcggtcatc cgcgagatca gatcgggcag cgtcatcagg cccgggcgga
     4321 cgaggacggt gttgaggacg ccaaaggcgg tttccatgcc gataatcccg ttgggggcgt
     4381 cggtgaacat caggtccttt tccatctcgg tatggggggc atggtcggtg gcgatgacct
     4441 cgatcgtgcc gtcgagcagg ccctcaatca atgcgaggcg gtcggattcc ggaccgatgg
     4501 ggggattgca cttggcattg gtgctgtagg tgcggcaggc ctcgacggtg agcgcgaggt
     4561 ggtggggggt gacctcgcac gagatgcgga cgccgcgggc gcgggcctgc cggatgatct
     4621 caaccgaggc gcgggtgctg acgtgcatga cgtgaatccg cccgccggtg aattccgaga
     4681 gctcggcgtc gcgggctacc tgaatgctct cggcgaccga gggccagccg cgcaagccga
     4741 ggcg
//
LOCUS       DAOPCO010000309         4733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15048_length_4733_cov_5.719752, whole genome shotgun sequence.
ACCESSION   DAOPCO010000309 DAOPCO010000000
VERSION     DAOPCO010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15048_length_4733_cov_5.719752"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /gene="fliD"
                     /locus_tag="PLS90_15325"
     CDS             complement(<1..1508)
                     /gene="fliD"
                     /locus_tag="PLS90_15325"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPK03818.1"
                     /translation="MSFASGVGISTGIDYNAMIRQIRSINSRPISLIRQNQSKLQQKQ
                     QALGVIKQALLNLKGKAESLNSSGSFLSLSATSTDGNVLLANASNEAAIGDYSVKVRQ
                     LAQAHRIASQGVADIDATPIAAGAGKFEFKVGDGATVAIDVTNGMSLRQLRDAINNQN
                     AGVRASIINDGTASHANRLVLTATSSGAANSIAIVANDTSLNLGATTIEEAYAATGNA
                     FDGTVTSSGVYTGTTTRNIVVQITEAGAVGAAKFKVSYDGGVTWSADNAFTTSAGPQD
                     ITGALGEGVMLAFGAGTQDFAVGDRFTIDAFAPELQKAQDALIEVDGVTVSRATNVFT
                     DVIQGVTLTARKVSEETQVIKVLNSNNSVQTKLQDLVNAYNDVVEKVQELTKYDKETN
                     ERAPLFGDSAVNSILSGLRSRVIGTVPGLETYNALSQIGITLDSTGKLQMDSAKVNSA
                     LNENLDAVKALFIQGATSGSSSIKFKAATSATTPGAYDVVITQAAEQAAISGA"
     gene            2134..2697
                     /locus_tag="PLS90_15330"
     CDS             2134..2697
                     /locus_tag="PLS90_15330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03819.1"
                     /translation="MIRPRVCVMDPDEHTRQLAGKTLGKHCDLLFTSPRALIDALEIF
                     EPDLLITEVDLPELSGFDLISIVQSDEHFRRIPIMVYSAVADLDSQKLAYRLGAMHFQ
                     VKPCRPSQFFKAAALFMRLASGEHPVRSHTVEEAERLIAERARAGEIHPLLSHVLAVR
                     HHGEEAAGHFERAVRASVARSHPKSRK"
     gene            complement(2725..3612)
                     /gene="folD"
                     /locus_tag="PLS90_15335"
     CDS             complement(2725..3612)
                     /gene="folD"
                     /locus_tag="PLS90_15335"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPK03820.1"
                     /translation="MSARLIDGKAIAQTIREELKAKVAATTGAGVRPPGLAVLLVGDN
                     PASAVYVRSKVKACEEAGFHSVKIDLPADTTQETLLAEIDKLNRDPKIDGILCQLPLP
                     KGLDENAVILAIDPDKDVDGFHPINAGRLAVGLPGFVPCTPLGCKELLIREGIETEGR
                     NVVVLGRSNIVGTPMALLMMQKAKGANATVTVCHSRTKDLPGECRRADILIAAIGQAN
                     FVKGNWVKPGATVIDVGINRVDDPSAPKGSRLVGDVDFAAVREVAGAITPVPGGVGPM
                     TIAMLLANTCQSWQRRCCG"
     gene            complement(4028..>4733)
                     /locus_tag="PLS90_15340"
     CDS             complement(4028..>4733)
                     /locus_tag="PLS90_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK03821.1"
                     /translation="DEPVRTSQVNVMGTIQLLEAARAAGVRRVVYAASSSAYGDQEQP
                     TKDEGLLPAPLSPYAAAKLAGEYFCQAYSRSMGLETVCLRYFNVFGPRQDPTSTYSAV
                     IPLFVTAVIEGRQPIIYGDGTQSRDFTYVENNVRANILAATTPKPVAGRVINVACGAS
                     YSLLELFDVIRRYFDSEIEPEFAPPRPGDVKHSLADITLARELLGFEVTVDFHEGLRR
                     TMEWYLAHREAWAAGG"
BASE COUNT          773 a         1646 c         1455 g          859 t
ORIGIN      
        1 gccccgctga tcgccgcctg ttcggccgcc tgcgtaatga cgacgtcgta ggcgccgggc
       61 gtcgtcgccg acgtggccgc cttgaacttg atcgacgagc tgccgctggt cgccccctgg
      121 atgaacagcg ccttgaccgc gtccagattt tcattcagcg cgctgttgac cttggccgag
      181 tccatctgga gtttgccggt cgaatcgagc gtaatcccga tctggctcag cgcgttgtag
      241 gtctccagcc ccggcacggt gccgatcacg cgcgagcgca gcccggagag gatcgagttg
      301 acggccgaat cgccgaacag cggcgcccgc tcgttcgtct ccttgtcgta cttcgtcagt
      361 tcctgcacct tctcgacgac gtcgttgtag gcgttcacca ggtcctgcag cttcgtctgc
      421 acgctgttgt tgctgttgag gaccttgatg acctgcgtct cctcgctgac cttcctggcc
      481 gtcagcgtca cgccctggat cacgtcggtg aagacattgg tcgcgcggct gaccgtgacg
      541 ccgtcgacct cgatcagcgc gtcctgggcc ttctgcagct cgggcgcgaa cgcatcgatc
      601 gtgaagcggt cgccgacggc gaaatcctgc gtccccgcgc cgaacgccag catcaccccc
      661 tcgcccaggg cgccggtgat gtcctgcgga ccggccgaag tggtgaaggc attgtcggcg
      721 ctccacgtca cgccgccgtc gtaggaaacc ttgaacttgg ccgcgccgac cgcgcccgct
      781 tcggtgatct gcaccacaat gttccgcgtg gtcgtccccg tgtagacgcc cgaggaggtc
      841 accgtgccgt cgaacgcatt gccggtcgcg gcgtaggctt cctcgatcgt cgtcgcgccc
      901 aggttgagcg acgtgtcgtt ggcgacgatt gctatcgagt tggccgcgcc ggaactggtc
      961 gccgtcagca ccagccggtt ggcatggctg gccgttccgt cgttgatgat gctcgcccgc
     1021 acgccggcgt tctggttgtt gatcgcgtcg cgcaactggc gcagcgacat cccgttggtc
     1081 acgtcgatcg cgaccgtcgc cccgtcgccg accttgaact cgaacttgcc cgcccctgcc
     1141 gcgatcggcg tcgcgtcgat atccgccacc ccctgcgacg cgatccggtg ggcctgcgcg
     1201 agctgccgca ccttgactga ataatcgccg atggccgcct cgttggacgc attcgccagc
     1261 agcacattgc cgtcggtcga cgtcgccgac aggctcagga agctccccga gctgttcagg
     1321 ctttcagcct tccccttcaa attgagcagc gcctgtttga tgacccccag cgcctgttgc
     1381 ttctgctgca gtttggactg gttctggcgg atcaggctga tcgggcgcga attgatcgac
     1441 cggatctgcc ggatcatggc gttgtagtcg atgccggtgg aaatgccgac gcccgaggcg
     1501 aatgacatgc ttcgcggtcc ttccgttcca tacgcggggc cggcgcgcgc cgctgcccac
     1561 agccccttct ctgacgttat cgtccgccgg cggcgggaac cttaagccga agtgcgaccg
     1621 attcaggccg ggcgaggcgg gcgctcctcg cgcgggctgc catcgggccc gattgccgga
     1681 tccgggcggg ccggccgacc gccgctgaaa tcgcataaat ctattacaat caattattta
     1741 catcgattca agggttggcg cgccagtccg ccttgcggaa aaaaaattgg cggcccaggg
     1801 gggagttgtg ctctaataat cgggtgttac ccgaacgcga ggagcctctc ccttggaagt
     1861 caacacggac tgttgcgggg ggatctgaat gtttcggaac gctgccagtc gatttcattt
     1921 catgcgcgga ccgcggcgcc ccttcgccgc tcagccgctc caccggtgct ccagccgtcc
     1981 tggagtcgtc cgcacgacgc cccctcacct tgaaccccct ttggtttgat gagagtttct
     2041 cgggagccgg gaacgagggc gagaaccgaa ctccgagcct ttgagtgaga tctgcatgcc
     2101 gcgatgcgcg gcatcccgcc ggaggttgcc aagatgattc gaccacgcgt ttgcgtcatg
     2161 gaccccgatg aacacacccg ccaactggcc gggaaaacgc tcggcaaaca ctgcgatctg
     2221 ctcttcacat cgccgcgcgc gctcatcgat gcgctggaga tcttcgaacc cgacctgctg
     2281 atcaccgagg tggatctgcc cgagctcagc ggattcgacc tgatcagcat cgtccagagc
     2341 gacgagcact tccgccgtat cccgatcatg gtctattcgg cggtggcgga tctggacagc
     2401 cagaaactcg cctaccgcct cggcgcgatg cacttccagg tcaagccctg ccggccctcg
     2461 caattcttca aggcggcggc gctgttcatg cggctggcca gcggcgagca tcccgtccgt
     2521 tcacacacgg tggaggaagc cgaacgcctg attgcggaac gcgcgcgcgc gggcgagatc
     2581 cacccgctgc tcagccatgt gctggcggtc cgccatcacg gcgaggaggc ggcggggcat
     2641 ttcgagcgcg cggtgcgcgc ctcggtcgcc cgcagccatc cgaaatctcg caaatagccg
     2701 tccggggccc ggccccccgc gccgtcagcc gcagcagcgc cgctgccagc tctggcaggt
     2761 attggccagc agcatcgcga tcgtcatcgg cccgacgccg ccgggcaccg gcgtaatcgc
     2821 gccggccacc tcccggaccg ccgcgaagtc cacatcgccc accagccggc tccccttggg
     2881 cgccgagggg tcgtccaccc gattgatgcc gacgtcgatc accgtcgcgc ccggcttgac
     2941 ccagttgccc ttgacgaaat tggcctgacc gatcgccgcg atcaggatgt ccgcccgccg
     3001 acattcaccc ggcaggtcct tcgtgcgcga atggcagacc gtcaccgtcg cgttggcccc
     3061 cttcgccttc tgcatcatca gcagggccat cggcgtcccg acgatgttgg accggcccag
     3121 cacgacgacg ttgcgcccct ccgtctcgat tccctcgcgg atcagcagtt ccttgcaccc
     3181 cagcggcgtg cacggcacga atccgggcag tcccaccgcc agccggccgg cgttgatcgg
     3241 atggaaccca tcgacgtcct tgtcgggatc gatcgccaga atcaccgcgt tctcgtccag
     3301 acccttgggc agcggcaact ggcagagaat gccgtcgatc ttcgggtccc ggttcaactt
     3361 gtcaatctcg gcgagcaggg tctcctgcgt cgtgtcggcc ggcaggtcga tcttgaccga
     3421 atggaacccg gcctcctcgc acgccttgac cttgctgcgc acgtagacgg cgctggcggg
     3481 gttgtcgccc accaggagga ccgccaggcc gggggggcgc acgccggccc cggtcgtcgc
     3541 ggccactttg gccttgagtt cctcgcggat ggtttgcgca atcgccttgc cgtcaatcag
     3601 tcgcgcgctc atgggttctc tcctatcagg atgactgcat ccgaagtcgc gccccacctt
     3661 acccgaaccg accgggcttt ccaagagcga agcgcaccgc gacgcacgac gcgaccaaac
     3721 gccggccccg gtggcgattc tcaaccgaaa ccggcaattc acaacgaggc atgaatatgg
     3781 cgggctgtca gcggccgatt cgaatggccg cgggattacc ggaccgatca gcaggccagt
     3841 cccgccaggg acggcagact tcagcccgtc gcttcagtgg cggggaaatg gatagaaaaa
     3901 aacgaaagag tcccgcaggg acggcagaac cgtcttccgt cccttctgtc cctcatgtcc
     3961 cttctgtccc tcgcaatcac gaggacgagc gacgaaaagc cgaattcctt gtgcggggtg
     4021 cggggaatca accacccgca gcccaggctt cacggtgggc aaggtaccat tccatcgtgc
     4081 ggcgcaggcc ctcgtggaaa tcgacggtga cctcgaagcc cagcagctcg cgcgccagcg
     4141 tgatgtcggc cagcgaatgc ttgacgtcgc cggggcgcgg cggcgcgaat tccggctcga
     4201 tctccgaatc gaaatagcgc cggatcacgt cgaacagctc gagcagcgaa tacgaggcgc
     4261 cgcacgccac gttgatcacc cgccccgcga ccggcttcgg cgtcgtcgcc gccaggatgt
     4321 tcgcccgcac gttgttctcc acgtacgtga agtcgcgcga ctgcgtcccg tccccataaa
     4381 tgatgggctg ccgcccctcg atcaccgccg tcacgaacag cggaatcacc gcgctgtagg
     4441 tgctcgttgg atcctggcgc ggcccgaaca cgttgaaata acgcagacag acggtctcca
     4501 gccccatcga ccggctgtag gcctggcaga agtattcccc cgccagcttg gccgccgcgt
     4561 agggcgaaag cggcgccggc agcagccctt cgtccttcgt cggttgctcc tgatcgccat
     4621 aggccgagga cgaagcggca tagacgacgc ggcgcacgcc ggccgcccgc gccgcctcga
     4681 gcaactggat cgtgcccatc acgttgacct ggctggtccg caccggctca tcc
//
LOCUS       DAOPCO010000310         4682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15251_length_4682_cov_4.926734, whole genome shotgun sequence.
ACCESSION   DAOPCO010000310 DAOPCO010000000
VERSION     DAOPCO010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15251_length_4682_cov_4.926734"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLS90_15345"
     CDS             complement(<1..375)
                     /locus_tag="PLS90_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03822.1"
                     /translation="MALSRFLLCAFAAFSLAGPVAFSAEVKVQWLFNFDAAAAGEPLT
                     QYEPQPSAGEVAPQGPITAVHAGGTPPTIRRDDSGGYLELDGTHPEGGAGLRFSHPVD
                     GEASRIFEALVRPAAGAAGGYDW"
     gene            complement(601..1572)
                     /locus_tag="PLS90_15350"
     CDS             complement(601..1572)
                     /locus_tag="PLS90_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPK03823.1"
                     /translation="MDATDSNKQRYLATLRGEIPDRVPLHETILNGRYLRHVLGECDY
                     EASHEMPPELQIPLLRRIGLDMASLGFYWRPTALNTWARLEEEVFPPLDGFLERVDRH
                     LAALRGEPLGLLVYVHGPLDTTYLSMGYMEFFMSVNTDPALVEAVMDRFTAYHRQLIE
                     ELVKRPIDVIQIVDDIAMKQGTFLEPRQQRRLWLPRMEQLIEPIRKAGIPMQFHSDGN
                     VEAFIEDVIAMGFSAINPIEPLCNDIREVKRRWGDRITLMGNLDIGGVLSNGTPEQVE
                     AEMRDLLRDLMPGGRYIAMSGSSIADCVDPRNYDAMVRTVKRHGRYG"
     gene            complement(1679..3238)
                     /locus_tag="PLS90_15355"
     CDS             complement(1679..3238)
                     /locus_tag="PLS90_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPK03824.1"
                     /translation="MHTLDFLIVYAYFALIAVISLWVTRGQKTTSKYFIADRTIPAWA
                     VAFTLMATMISSGAIVGTPGTVYERGMILLLGHLMLPLVLLFVAYFIVPFYRHVVGMS
                     AYEFVNKRFGAFARVYASLGFLADRIFDLGVTLITTAIAVQVMTGWEMMPVIIGIGIF
                     TIIYTTVGGIEAVVWTNAVQGAIILGGMLLVPAFLLFSPEAGPPGAVVAEAWRGGRFD
                     LGRFDLSWDSLFDPDTTQWLFLLAFIIGWGRRYVSDQHMVQRYLIAKSDRAARQGAFW
                     GAFLCVPIWVTFMFIGACLYGYYQLTPHEPPAIADQIAPYFIVNAMPTGLVGLILAAI
                     MAASMSSVSADLNSVATVATTDYFSALRPLSNDKARLIFGRLMVVVFGILATLTAILL
                     IPREGIKPLMERGVTIAAIISAGSLGLFLLGFLTRRATRLGCYSGIAACILFSAWGLL
                     TSGGEDRILDTGVNFTMNPILIGVFGHFVLFGVGYLVSLIFGGYRPDDVEQLTIFRRK
                     DLDLAEATPGA"
     gene            4078..>4682
                     /locus_tag="PLS90_15360"
     CDS             4078..>4682
                     /locus_tag="PLS90_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03825.1"
                     /translation="MPDRSPLIRRVLPLLLALTSLAGAPRAEEPALRVTPDSLNFVVA
                     TGQTTGPLQAVSFWLSDRSRHWRLTYDADWLAIDRLTGDGSVGQMTARFRVNHAGLAP
                     GRHATTVVAELDGMPETRVELPVNLRVFDLPVIRDVGGRKQLFIDQRFIEESENVVLK
                     VNPPTKIGQTLDENGEVVNLGFIMRVLEDEGKYLIYHGGER"
BASE COUNT          918 a         1498 c         1478 g          788 t
ORIGIN      
        1 ccagtcgtag ccgccggcgg cgccggcggc cgggcgcacc agcgcctcga agatgcgcga
       61 cgcctccccg tcgaccggat ggctgaaccg cagccccgcg ccgccctcgg ggtgcgtgcc
      121 gtcgagctcc aggtagccgc ctgagtcatc ccggcgaatc gtcggaggcg taccaccggc
      181 atgcaccgcc gtgatcggcc cctggggcgc cacctcgccg gccgagggct ggggttcgta
      241 ctgcgtcagc ggctcgccgg ccgcggccgc atcgaaattg aacagccact ggaccttgac
      301 ctcggccgaa aaagcgaccg ggcccgcgag ggaaaacgcg gcgaaggcgc agagaagaaa
      361 ccggctgaga gccatgggtg cgttgactcc gggagagtga tccgacggtt ggcggcggcg
      421 attgatgcgg tcgaaggagt ccacggctcg accgccgact gggacgaaac ctcgcccgcg
      481 acggcgccgg cgaagctgtc cagtccgatc aaagctgatg gagagattat agatcaaata
      541 aaagggctca gtcaaccata attgtctgat ataaaaaccg ggaggcggaa aatcccccgt
      601 ctatccgtat ctgccatggc gcttgaccgt ccgcaccatc gcgtcatagt tgcgcgggtc
      661 gacgcagtcg gcaatgctgc tgccgctcat cgcgatgtag cgcccgcccg gcatcaggtc
      721 gcgcagcagg tcgcgcatct cggcctcgac ctgctccggg gtcccgttgg acagcacgcc
      781 gccgatatcc aggttgccca tcagggtgat gcgatcgccc cagcgccgct ttacctcgcg
      841 gatgtcgttg cacagcggct cgatgggatt gatcgcggaa aaacccatcg caatgacgtc
      901 ttcaatgaag gcctcgacgt tcccgtcgct atggaactgc atggggatgc cggccttgcg
      961 gatcggctcg atcaactgct ccatgcgcgg gagccacagc ctgcgctgtt ggcgcggctc
     1021 aaggaacgtc ccctgcttca tcgcgatatc gtcgacaatc tggatgacgt cgatcgggcg
     1081 cttgaccagt tcctcgatca actggcggtg ataggccgtg aagcggtcca tgaccgcctc
     1141 gacgagggct ggatccgtat tgacggacat gaaaaactcc atgtagccca tggaaagata
     1201 ggtcgtgtcg agcggaccat ggacatagac gagcagcccg agcggctcgc cgcgcagcgc
     1261 tgccagatgg cgatcgaccc gctccaggaa cccgtccagc ggcgggaaga cctcctcctc
     1321 cagccgcgcc cacgtattga gcgcggtggg gcgccagtag aagccgagcg aggccatgtc
     1381 gagcccgatc cggcgcagca gcggaatctg cagttcgggg ggcatctcgt gcgaggcctc
     1441 gtaatcgcac tcgcccagga catggcgcag gtagcgaccg ttgagaatcg tctcgtgcag
     1501 cgggacccgg tcggggattt ccccgcgcaa ggtcgcgaga tagcgctgct tattggagtc
     1561 cgtcgcatcc acggcgcgcc aaccctttca tctcagacgt gatgatcatg gctcccagaa
     1621 catgaaagcc aatccgtcgg aactggtccc gcaattcgga cggctccccc tggccggttc
     1681 aggcgcccgg cgtggcttcg gccagatcga gatccttccg gcgaaagatg gtgagctgtt
     1741 ccacatcgtc gggacgatac ccgccgaaga tcaggctgac caggtagccg acgccgaaca
     1801 ggacgaaatg gccgaagacc ccgatcagga ttggattcat cgtgaaattc acgcccgtgt
     1861 ccaggatgcg atcctcgccg ccgctggtca gcagtcccca ggccgagaaa aggatgcagg
     1921 cggcgatgcc ggagtaacag ccgagccggg tggcccggcg ggtgaggaag ccgaggagga
     1981 acaggccgag cgagccggcc gagatgatcg ccgcgatcgt cacgccgcgt tccatcagcg
     2041 gtttgatgcc ttcgcgcggg atcagcagaa tcgcggtgag ggtggcgagg atgccgaaca
     2101 caaccaccat caaccggccg aagatcagtc gcgccttgtc gttcgacagc ggccgcaggg
     2161 ccgagaaata gtcggtggtg gcgaccgtgg cgacgctgtt cagatcggcg ctgaccgagg
     2221 acatcgaggc ggccatgatc gccgccagaa tcagtcccac caggccggtc ggcatcgcgt
     2281 tgacgataaa gtagggggcg atctggtccg cgatcgccgg cggctcgtgc ggcgtcaatt
     2341 gataatagcc atacaggcag gcgccgatga acatgaacgt cacccagatc gggacgcaga
     2401 ggaacgcgcc ccagaaggcg ccctggcgcg cggcccggtc ggacttggcg atcaggtagc
     2461 gctgcaccat gtgttgatcg gagacatagc gccgccccca gccgatgatg aacgcgagca
     2521 ggaacagcca ctgggtcgtg tcgggatcga acaggctgtc ccaggaaaga tcgaagcggc
     2581 ccagatcgaa acggcccccg cgccaggcct cggcgacgac ggcgccgggc ggcccggctt
     2641 ccggcgagaa cagcaggaaa gcagggacga gcaacatccc gccaagaatg atcgccccct
     2701 ggacggcgtt ggtccagacc accgcttcga tgccgccgac cgtcgtgtaa ataatcgtga
     2761 aaatgccgat gccgataatc accggcatca tttcccagcc ggtcatcacc tgcacggcga
     2821 tcgcggtcgt gatcagcgtg acgccgaggt cgaaaatgcg gtccgccagg aagcccagcg
     2881 acgcatagac gcgggcgaac gcgccgaagc gcttgttcac gaactcatac gcgctcatcc
     2941 cgacgacgtg gcggtagaac gggacaataa agtaggcgac gaacagcagc acgagcggca
     3001 gcatcaggtg ccccagcaac agaatcattc cacgctcgta gacggttccg ggcgtcccga
     3061 cgatggcgcc gctgctgatc atcgtggcca tcagcgtgaa ggccacggcc catgcgggga
     3121 tcgtgcgatc ggcgatgaaa tatttcgagg tggtcttctg accccgggtg acccagaggc
     3181 tgatcacggc gatgagggcg aaataggcgt aaacaatcag gaaatcgaga gtatgcatag
     3241 accgtccaca attgtcggat tcatgggtga ttcagtcgcc tgatggcatg ccgcccggca
     3301 agccggcgcc tccccggtca cttttcgagc tggccgggat tgttcacccg gtatgagtcg
     3361 cacccagccc ttggcactcg gcggccattt tcggcaattt gcgcaccccg caccatggga
     3421 tttccccaac ggagtccgcc gtgatcggat cgtgttcgct gacgagacag gccgacatcg
     3481 catgccggcc tcgtccggga tgccggcgcg ccgcccgcca gaggtggcgt cgacgttcag
     3541 atccagccgg ttgagggttt ccgttcttga atttagagga tgcgaggtgg ctttgggatt
     3601 tcaaaactcg aagctggtgc tcctcccgtc aggaaccccc tgtcgttgac cacgacacgc
     3661 atcctctgtt tcatcagcgc aaaggcacga cttcagcgaa tggtaaccag gatttgggta
     3721 acatgtcgct ttcttcgctg tcaaccccta tttgtatgat atgttctgag ttctcgccaa
     3781 atgatacgtt aataccgaaa tccgacggca agaggcgacc gcctgaacct ggaagcgcct
     3841 cgctcccgcg tggcacggcc ttgggcgaac cccactccca cccgggtaga cttctctcca
     3901 ggccttcccg tacctggcct ctttccacat tttcgctggc agggacttcg cagcgcgcct
     3961 tgcgcgccaa acttttgtca gatgagttgt gtccattggg gcgatacggc gcggtcgcgc
     4021 aggagctgtc ctgtccgcca cccgtcaccg ccactcacgt cgcaaggaat gatactcgtg
     4081 cccgatcgaa gtcccctgat ccgccgcgtc ctgcccctgc tgttggcgct gacctcgctt
     4141 gccggcgcgc cgcgggccga agagcccgcc ctgcgcgtca cgcccgattc cctaaacttc
     4201 gtcgtcgcca ccggtcagac caccggcccc ctccaggccg tcagcttctg gctgtccgac
     4261 cgctcgcgcc actggcgcct gacatacgat gcggactggc tggcgatcga ccggctgacc
     4321 ggcgacggca gcgtcgggca gatgaccgcc cgcttccgcg ttaaccatgc gggcctggcg
     4381 cccggccggc atgccaccac cgtcgtcgcc gagctcgacg gcatgcctga gacgcgcgtg
     4441 gagctgccgg tcaacctgcg cgtgttcgat ctgccggtga ttcgcgatgt cggcgggcgc
     4501 aagcagctct tcatcgatca gcggttcatc gaagagagcg aaaacgtcgt gctcaaggtc
     4561 aatcctccga caaaaatcgg ccagacgttg gacgaaaatg gcgaggtcgt caatctcggc
     4621 ttcatcatgc gggtcctcga ggacgagggc aaatatctca tctaccacgg cggcgaacga
     4681 at
//
LOCUS       DAOPCO010000311         4654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15351_length_4654_cov_5.191781, whole genome shotgun sequence.
ACCESSION   DAOPCO010000311 DAOPCO010000000
VERSION     DAOPCO010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15351_length_4654_cov_5.191781"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /gene="rpsQ"
                     /locus_tag="PLS90_15365"
     CDS             complement(<1..131)
                     /gene="rpsQ"
                     /locus_tag="PLS90_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPK03826.1"
                     /translation="MANQTETRGNVKERVGRVVSSKMDKTVVVAVDRMVKHPLYGKT"
     gene            complement(182..388)
                     /gene="rpmC"
                     /locus_tag="PLS90_15370"
     CDS             complement(182..388)
                     /gene="rpmC"
                     /locus_tag="PLS90_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPK03827.1"
                     /translation="MQAAEYRKMSIEELEDQVDQLRTELFNLRVKNTTKELQDTSRIR
                     RTRRDLARVLSVLTEKRRPAEARA"
     gene            complement(404..817)
                     /gene="rplP"
                     /locus_tag="PLS90_15375"
     CDS             complement(404..817)
                     /gene="rplP"
                     /locus_tag="PLS90_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPK03828.1"
                     /translation="MLIPKRVKHRKVQKGRRRGLAWRGSSLSFGEYGLKALDDAWVTN
                     RQIEAARIAITRHVKRGGKLWINVFPDKPLTKKPAETRMGKGKGAPELWVAVVKPGRI
                     LFEIEGVSEELAREAMRLASHKLPIKTTFVARAAE"
     gene            complement(878..1534)
                     /gene="rpsC"
                     /locus_tag="PLS90_15380"
     CDS             complement(878..1534)
                     /gene="rpsC"
                     /locus_tag="PLS90_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPK03829.1"
                     /translation="MGQKVNPISNRLGITRTWDSRWYTTKFKYAEFLHEDIRLRNTIR
                     KQFYHAGIAAITIERAGSRCKITVATGRPGIIIGPKGSEIENLRKNLEQMTGKTVTIN
                     IEEVKRTELSAQLVAENVAGQLMRRIGFRRAMKKTMQSSMDAGALGIKIHCAGRLAGA
                     EMARREWYRRGRVPLHTLRAEIDYGFAEASTKFGKIGVKVWIFKGEKFKEVTRKRAAA
                     "
     gene            complement(1551..1940)
                     /gene="rplV"
                     /locus_tag="PLS90_15385"
     CDS             complement(1551..1940)
                     /gene="rplV"
                     /locus_tag="PLS90_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPK03830.1"
                     /translation="MSKARAARKPNLGAVVTSATVRFTRVPPRKARIVADLLRGLTVE
                     EAQEQLQLVHRPSAAPVISNLLKSAVANARNGERAEGVDPEELIIGEIFVDGGPMLKR
                     YRPKAFGRANIIRKRMSHITIRLYREA"
     gene            complement(1969..2253)
                     /gene="rpsS"
                     /locus_tag="PLS90_15390"
     CDS             complement(1969..2253)
                     /gene="rpsS"
                     /locus_tag="PLS90_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPK03831.1"
                     /translation="MARSLKKGPYVDEKLAAKIRELNITGQKRVVRTWARASMITPDM
                     VGHTLAVHNGKQFMTVYIDENMVGHKLGEFAPSRTFRSHGGKQAKAAGVR"
     gene            complement(2271..3101)
                     /gene="rplB"
                     /locus_tag="PLS90_15395"
     CDS             complement(2271..3101)
                     /gene="rplB"
                     /locus_tag="PLS90_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPK03832.1"
                     /translation="MPIKTYKPVTPSRRTMSTVDYSVLTPKEPEKSLTEPLSKKGGRN
                     NTGRISVRRRGGGHPRKYRVIDFKRLKDGVPAKVAAIEYDPNRSAFIALLHYADGAKS
                     YIIAPEGLKVGHKVMSGPGADIAPGNVLPLRLIPVGTTVCCIEMKPGKGAQLARAAGA
                     GVQLMAREGDRATLRMPSSEMRFVHADCRAMIGTVGNSDHGNVSLGKAGRTRWLGRRP
                     KVRGVAMNPVDHPHGGGEGRTSGGRDPVTPWGKPTKGAKTRNARKQSSRDIIRRRREK
                     "
     gene            complement(3146..3436)
                     /gene="rplW"
                     /locus_tag="PLS90_15400"
     CDS             complement(3146..3436)
                     /gene="rplW"
                     /locus_tag="PLS90_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPK03833.1"
                     /translation="MSKHPYQILDRPLLTERATDRQTMARPQYTFKVAVDSNKVEIRR
                     AIEQAFNVRVVGVNTVMVKGKRKRLRTAKLGRRPDWKKAIVTLAEGDSINLI"
     gene            complement(3433..4056)
                     /gene="rplD"
                     /locus_tag="PLS90_15405"
     CDS             complement(3433..4056)
                     /gene="rplD"
                     /locus_tag="PLS90_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020155513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPK03834.1"
                     /translation="MATVTVRKADGSTGQTLELNEAVFAVEPNPHCVRQALTQYLANQ
                     RRGTHATRTRGRVSGGGKKPYRQKGTGRARQGSTRATQWRGGAIVFGPQPRDYSFRIN
                     RKVRRNAFCSIFSELLAGGRLVVIDDFGVTAPKTRQVADLLRRLQLEGKVLIVTNATD
                     EPLALSARNIPGVTPINADNLNVYDLLYHDWVLTTPDVVKRVEATYQ"
     gene            complement(4109..>4654)
                     /gene="rplC"
                     /locus_tag="PLS90_15410"
     CDS             complement(4109..>4654)
                     /gene="rplC"
                     /locus_tag="PLS90_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPK03835.1"
                     /translation="QKDGYDALQVGFDPRPERTVTRPMNGHFKKAGVTPQRLVREFRI
                     DGEGGYQTGQTLDLSLFEVGDKVDVVGVSKGRGFAGSQKRHHSSRGPETHGSNYHRMS
                     GSMGASADPSHVHKGKKLPGHMGHARVTVQNIRVVDVDRENHLILVRGSVPGHPNGYV
                     MLRKSLKMLRRRQARKAAGKG"
BASE COUNT          596 a         1492 c         1622 g          944 t
ORIGIN      
        1 atcgtcttgc cgtagagcgg gtgcttgacc atgcggtcga cggcgaccac gacggtcttg
       61 tccattttgg agctgacgac gcgcccgacg cgttccttga cgttgccgcg ggtttcggtc
      121 tgattggcca tagccctgct tcactccgtt tcgcgcggtc cggcggaccg cggcatcaat
      181 ctcaggcccg cgcttcggcc ggccgccgtt tctcggtcaa cacactgagc acccgcgcca
      241 gatcgcggcg ggtgcgccgg atccgcgagg tgtcctgcag ttccttggtc gtgttcttga
      301 cgcgcaggtt gaacagctcg gtgcggagtt gatcgacctg atcttccagt tcctcgatgc
      361 tcattttgcg atattcggcc gcttgcatag cgttctgctc cgtttactcc gcggcgcgcg
      421 ccacgaaagt ggtcttgatc ggcaacttgt gcgaggccag gcgcatcgcc tcgcgggcca
      481 gttcctcgct cacgccttcg atctcgaaca ggatccgccc cggcttgacc acggcgaccc
      541 acagttccgg ggcgcccttg cccttgccca tgcgggtttc ggccggcttc ttcgtcagcg
      601 gcttatccgg gaagacgttg atccacagct tgccgccgcg cttgacgtgg cgggtgatgg
      661 cgatgcgcgc ggcctcgatc tggcggttgg tgacccaggc atcgtcgagg gccttgaggc
      721 cgtattcgcc gaagctcagg ctggaaccgc gccaggccag tccgcggcgg cggcctttct
      781 ggaccttgcg gtgtttgact cgtttcggaa tcagcatgtc gtttctctgc ccggtggact
      841 agccggagcg cccaggctcc gccgtgcgtg ccggccgtca ggcggccgcg cgcttgcgcg
      901 tcacttcctt gaatttttcg cccttgaaga tccagacctt gacgccgatc ttgccgaact
      961 tggtgctggc ctcggcgaag ccgtaatcga tctcggcgcg cagggtgtgg agcggcacgc
     1021 ggccgcgacg ataccattcg cgccgggcca tttcggcgcc cgccagccgg ccggcgcagt
     1081 gaatcttgat gcccaaggcg ccggcgtcca tcgaggactg catcgttttc ttcatcgcgc
     1141 ggcggaagcc gatgcggcgc atcaactgac cggcgacgtt ttcggcgacc agttgcgccg
     1201 acagttccgt ccgcttgacc tcctcgatgt tgatcgtgac ggttttgccg gtcatctgct
     1261 ccaggttctt gcgcaggttc tcgatctcgc tccccttggg gccgatgatg atgccggggc
     1321 ggccggtggc gacggtgatc ttgcagcgcg aaccggcgcg ctcgatcgtg atggccgcga
     1381 tgcccgcatg gtagaactgc ttgcggatcg tgttgcgcag gcggatgtcc tcgtggagga
     1441 actcggcgta tttgaacttg gtggtgtacc agcgcgagtc ccacgtgcgc gtgatgccca
     1501 gtcggttgct gatggggttg accttctggc ccatggacct ctcccgttcc tcaagcttcc
     1561 ctgtacaggc gaatggtgat gtgcgacatc cgtttgcgaa tgatgttggc gcggccgaag
     1621 gccttgggcc ggtagcgttt gagcatcggc ccgccgtcga cgaaaatctc gccgatgatc
     1681 agctcctcgg ggtcgacgcc ctcggcgcgc tcgccgttgc gggcattggc cacggccgat
     1741 ttgagcaggt tgctgatgac cggggcggcc gacgggcgat ggaccagttg cagttgctcc
     1801 tgggcctcct ccaccgtcag gccgcgcagc aggtcggcca cgatgcgcgc cttgcgcggc
     1861 ggcacgcggg tgaagcggac cgtcgccgag gtgaccacgg cgcccaggtt gggtttgcga
     1921 gctgcgcgtg ctttggacat cgttgggatt ctcctgtcgg ccgcgggctc agcgcacgcc
     1981 ggcggctttg gcctgtttgc cgccgtggct gcggaaggtg cggctggggg cgaattcccc
     2041 cagcttgtgc ccgaccatgt tttcgtcgat gtagacggtc atgaactgct tgccgttgtg
     2101 gacggccagg gtgtggccga ccatgtcggg ggtgatcatc gaggcgcgcg cccaggtgcg
     2161 gacgacgcgc ttctgaccgg tgatgttgag ctcgcggatc ttggccgcca gcttttcatc
     2221 gacgtagggg ccttttttca ggcttctggc catcgtctca cctcaatcgc ttacttctcg
     2281 cgccggcggc ggatgatgtc gcggctgctc tgcttgcgcg cgttgcgggt cttggccccc
     2341 ttggtcggct tgccccaggg ggtgaccgga tcgcgcccgc ccgaggtgcg gccctcgccg
     2401 ccgccgtggg ggtgatcgac ggggttcatg gcgacgccgc ggaccttcgg gcgccggccg
     2461 agccagcgcg tccggccggc cttgcccagg ctgacgttgc cgtggtccga gttgccgacc
     2521 gtgccgatca tggcgcggca gtcggcgtgg acgaagcgca tttcggagga gggcatgcgc
     2581 agcgtggcgc gatcgccctc gcgggccatc aactgcacgc cggcgcccgc cgcgcgggcc
     2641 aactgggcgc ccttgcccgg cttcatttcg atgcagcaga ccgtcgtgcc gacggggatg
     2701 aggcgcagcg gcaggacgtt gcccggcgcg atgtcggcgc ccggccccga catcaccttg
     2761 tgacccacct tgagcccctc gggggcgatg atgtagctct tggcgccgtc ggcgtaatgc
     2821 agcagcgcga tgaacgccga gcggttcgga tcgtactcaa tcgcggcgac tttggccggc
     2881 acgccgtcct tgaggcgctt gaaatcgatg acgcggtatt tgcggggatg gccgccgccg
     2941 cggcggcgca cggaaatccg cccggtgttg ttgcgcccgc ccttcttgct cagcggctcg
     3001 gtcagggact tttcgggttc cttgggggtc agcaccgaat agtcgaccgt gctcatcgtc
     3061 cggcggctgg gggtgacggg tttgtaagtc ttgatcggca tcgttcgttc tttccagcgg
     3121 tcgagcggcg ggcggcgccg cggcctcaga tcaggttaat gctgtcgcct tcggccagcg
     3181 tgacgatcgc tttcttccag tcggggcggc ggccgagctt cgccgtccgc aaccgcttgc
     3241 gtttgccctt gaccatgacg gtgttgacgc cgacgacgcg gacgttgaag gcctgctcga
     3301 tcgcgcggcg gatttccacc ttgttgctgt cgaccgccac cttgaacgta tactgcggcc
     3361 gcgccatcgt ctggcggtcg gtcgcgcgct cggtcagcag cggccgatcc aggatttggt
     3421 agggatgctt gctcattggt acgtcgcctc cacgcgcttg acgacgtcgg gggtcgtcag
     3481 cacccagtcg tgatagagca ggtcgtagac attgaggttg tcggcgttga tcggggtgac
     3541 gcccgggatg ttgcgcgcgg agagggccag cggctcatca gtggcgttgg tgacgatcag
     3601 gaccttgccc tcgagctgga gccggcgcag caggtcggcg acctggcgcg ttttgggggc
     3661 ggtgacgccg aagtcgtcga tgacgacgag ccggccgccg gcgagcagtt cgctgaagat
     3721 ggagcagaag gcgttacgcc ggaccttgcg gttgatgcgg aaggagtaat cgcgcggctg
     3781 agggccgaag acgatcgcgc cgccgcgcca ttgcgtggcg cgcgtggagc cctgccgcgc
     3841 ccggccggtg cccttctggc gatagggctt cttgccgccg ccgctgacgc ggccgcgcgt
     3901 gcgcgtcgca tgggtcccgc ggcgctggtt ggccagatac tgcgtcagcg cctggcggac
     3961 gcaatgcggg ttgggctcaa cggcaaacac cgcctcgttc agctccagcg tctgcccggt
     4021 tgatccgtcg gcttttcgca ctgtaaccgt tgccatggtc tcgtcccttg ccttcctcgc
     4081 ggttgggggg cgccgcgccg ttgccggctc agcccttgcc cgccgccttg cgcgcctgcc
     4141 ggcgccgcaa catcttcaga ctcttgcgca gcatgacgta gccgttgggg tggcccggca
     4201 ccgaaccgcg caccaggatc aggtgattct cgcggtcgac atcgaccacg cggatgttct
     4261 gcaccgtcac gcgggcgtgg cccatgtggc cgggcagctt cttgcccttg tggacgtgcg
     4321 aggggtcggc cgaggcgccc atcgaaccgg acatccggtg atagttggag ccgtgggtct
     4381 cggggccgcg gctggagtga tggcgctttt gcgagccggc gaagccgcgc cccttggaga
     4441 cgccgaccac atcgaccttg tcgccgacct cgaacaggct caggtcgagc gtctggccag
     4501 tctggtagcc gccctcgccg tcgatgcgga actcgcggac gaggcgctgc ggcgtgacgc
     4561 cggccttttt gaaatggccg ttcatcgggc gggtgacggt ccgctcgggg cgggggtcga
     4621 agcccacctg cagcgcatcg tagccgtcct tctg
//
LOCUS       DAOPCO010000312         4648 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15378_length_4648_cov_4.867842, whole genome shotgun sequence.
ACCESSION   DAOPCO010000312 DAOPCO010000000
VERSION     DAOPCO010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15378_length_4648_cov_4.867842"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..797
                     /locus_tag="PLS90_15415"
     CDS             177..797
                     /locus_tag="PLS90_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03836.1"
                     /translation="MERKAGRPQSLSDVLLQTRMRTQEQVRERFARYGDPVCEKIRQA
                     ANHPNGLGRLLERLGLACTPRFDQQCYLLQRFLNDHGPQAFLNRLGERARTGRILIAE
                     APPSAELGAPPAQTKPRAATPPPNGAWPKHTPPNWFDAHGNYVGPERRSGEDRRTGKD
                     RRGDIESIRKNRRYGGDRRKVVRRKEDRIKLGLEKPDPPQRKRQCG"
     gene            complement(1552..2556)
                     /locus_tag="PLS90_15420"
     CDS             complement(1552..2556)
                     /locus_tag="PLS90_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK03837.1"
                     /translation="MAFGPHQVLRDIDFSIWRGDRVAIVGLNGAGKTTLMKLMTGALK
                     PTAGEVIPGPSTTTEYFAQYDDEGLSPANTVFNEVNAVAPAGAEQQVRNLLGGFFFSG
                     EDADKKIAVLSGGERTRVRLAKMLFSGANTLLLDEPTNHLDLASRRTLEDAMLAFPGT
                     LIFVSHDRVFLERVPTRIFEIKDGRLRIFDGNYHDYARVLTAMGEESPLVERGAARGA
                     PAPPPRLRPTATGKSAPAPAAADEDDASLNREERKARQREERRLRRALEELEDRIAKI
                     EQRLTEIDHELMRPSVYSNPQAAARFGAEQRQLRAEHESLMARWEKQSEELAALGPIP
                     "
     gene            complement(2671..3555)
                     /locus_tag="PLS90_15425"
     CDS             complement(2671..3555)
                     /locus_tag="PLS90_15425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK03838.1"
                     /translation="MLTVNNLSKFIGARPLLEHINFMLQPGECATLVGANGSGKSTLL
                     RICAGEISPDAGEFTVPRDATIGYLPQHAELHSNRRLRDELRHVFEEVLSHQQEADDL
                     AHKLGELDPASDDYMRALDRFGHLHHEIERLGAYDMDSRIGRIAAGLGFRTADLDRPC
                     QEFSGGWRMRILLARLLLRNPDVLLLDEPTNHLDLETMIWLEDWIRSSDSTVLMVSHE
                     RAFMDNLAQRVLELHEGQLTIYRGNYTSYLAQREERWQQWSRAYANQQEEIARIQKFI
                     DRFRYKATKAVQVQSRIK"
     gene            3850..4569
                     /locus_tag="PLS90_15430"
     CDS             3850..4569
                     /locus_tag="PLS90_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HPK03839.1"
                     /translation="MNGVEPRERVRVLVDDREKTHGLVPAIAGLVGDRLTLTRLVAGD
                     IRIGEAYLIERKTAADFVASLLDGRLEHQLAALAGVHPAQALLIIEGTFTPAVLGGLA
                     PAEARRALLSIALDWRIPCLHAATAEETARWVALLVERVERPGRPFAGDFAAAQEVVP
                     PPTARFSGRRAGAGAHAVQQAALRRIPGLGARRAEALLRRFGSISALARATEAEIAAT
                     PGIGPERAAAVRRALAMPPPP"
BASE COUNT          745 a         1224 c         1886 g          793 t
ORIGIN      
        1 tttgtggaca ggattggcgc atttcgagag ttgaggacga gggacggggg aggtgttttt
       61 tgtgccaggg actcaggttg gcgccggggg gttgccgata aggagtgcga gggcggtccg
      121 ccgcgcgggg gagggagaat ttttaccggg tcgccggaag agagatgtca tcatcaatgg
      181 aacggaaagc gggccggccg caatcgctga gcgacgtgct gctgcaaacc cgaatgcgca
      241 cgcaggaaca ggtgcgcgag cgcttcgcgc gctacggcga tccggtgtgc gagaaaatcc
      301 gccaggcggc gaaccacccc aatgggctgg ggcggctgct ggagcgtctt ggtctggcct
      361 gcacacctcg gttcgatcaa cagtgctatc ttttacagcg atttttgaac gaccatggtc
      421 cgcaggcgtt cctcaaccgg ctgggcgagc gggcacggac ggggaggatt ctgatcgcgg
      481 aggcgccccc ctcggcggag ctcggggcgc cgccggcgca gacgaagccg cgggcggcga
      541 cgccgccgcc caacggggcc tggcccaagc atacgccgcc gaactggttt gatgcgcacg
      601 ggaactacgt gggtccggag cggcggtcgg gggaagaccg acgtacgggc aaggaccgtc
      661 gcggggatat cgaatcgatc cgcaagaatc gacgttacgg gggggaccgg cggaaggtgg
      721 tccggcgcaa ggaagaccgg atcaagttag gtttggagaa accggacccg ccgcagcgga
      781 agcgtcagtg cggttgagcg agcgaagacc gagggcgggg ctttaagtta gcggttgagt
      841 gagggcaggg ccggtgacgg gcggccggtg acggcgtgat gccgccgcgc cttcaatcca
      901 ctcccctcgc ctttgtttcg atttgcagtt cggaccatcc ttccaaaatc tccgtgctct
      961 gtttccaatt tcctggcggg aaattcgcgc cggcgatacc gctggtcgat tcgctgcgat
     1021 gtatccggca tccctgcggg atgggacgat aataggcggc cattgaccgg tggcgtcgct
     1081 gcgctcaacc accggctacc ttctggcatc cccgcgggat gccccccgcg aacatctctc
     1141 cacctgaccg aatgaatggc caaacccgcg ggatgcgggc ggtggcgatt cgatagcgat
     1201 ccgtcaccct gaagggatga aatggattgc ccgccctttg tttgactgag attcgcccgc
     1261 tgcggggatg ggagttatcg gcgatagcgt ccgcaagttc cgctgcgctc cacccggggc
     1321 gatataaatg tcgcccccgc tgggacggct cacgcggacg gctttggatg catttgaatc
     1381 agcggtgaag aagacggaat tctcgtccga attgctcgtg aaccggtggg taacggcgaa
     1441 ggggcgcaag ggcggaattt cgagagccga ggacgaggga cgagagccgg gagccgagat
     1501 tcggagtatg tctttggggg cgaggacgaa gacgagggtc gaggggacgg atcaggggat
     1561 ggggccgagg gcggcgagtt cctcggactg tttttcccag cgggccatca gggattcgtg
     1621 ttcggcgcgc agttggcgct gttcggcgcc gaagcgggcg gcggcctggg gattgctgta
     1681 gacgctgggg cgcatcaatt cgtggtcgat ctcggtcagg cgctgttcga ttttcgcgat
     1741 gcggtcctcg agttcctcga gggcgcggcg gagacggcgt tcctcgcgct ggcgggcctt
     1801 gcgttcctcg cgattgaggg aagcgtcgtc ctcgtcggcg gcggcggggg cgggcgctga
     1861 tttgcccgtt gcggtgggtc gcagccgggg gggcggcgcc ggggcgccgc gcgcggcgcc
     1921 gcgttcgacg agcgggctct cctcgcccat cgcggtcagc acccgcgcat agtcgtgata
     1981 gttgccgtcg aagatgcgca agcggccgtc cttgatttcg aagatgcgcg tggggacgcg
     2041 ttcgaggaag acacggtcgt gactgacgaa gatgagggtg ccggggaagg ccagcatcgc
     2101 gtcctcgagg gtgcggcgcg aggcgagatc gagatggttg gtcggttcgt cgaggagcag
     2161 ggtattggcg cccgagaaaa gcattttggc gaggcggacg cgggtgcgct cgccgccgga
     2221 gaggacggcg attttcttgt cggcatcctc gccggagaag aagaagccgc cgaggagatt
     2281 gcggacctgc tgttcggcgc cggcgggggc aacggcgttg acttcattga agacggtgtt
     2341 ggcggggctc aggccctcgt cgtcgtactg ggcgaagtat tcggtggtgg tggaggggcc
     2401 ggggatgact tcgccggcgg tgggcttgag cgcgccggtc atcagtttca tgagggtggt
     2461 cttgccggcg ccgttgaggc cgacgatggc gacgcggtcg ccgcgccaga tggagaaatc
     2521 gatgtcgcgg aggacctgat gcggtccgaa agccatggag agatggcggc cgacgaagac
     2581 ttccttggag ccgcggtcgg gcggggggaa tttgaaatgg atcgcggccg gttcgctggg
     2641 cgggggcggg atgcgttcga tcttctccag ttacttgatg cgcgactgca cctgaacggc
     2701 cttggtcgcc ttgtagcgga agcgatcgat gaacttctgg atgcgggcga tctcctcctg
     2761 ctggttggcg taggcccggc tccattgctg ccagcgttcc tcgcgctggg cgaggtagct
     2821 ggtgtaattg ccgcggtaga tcgtgagttg cccctcgtgg agttcgagga cccgctgggc
     2881 gaggttgtcc atgaaggcgc gttcgtggct gaccatgagg acggtggaat cgctggaacg
     2941 gatccagtcc tcgagccaga tcatcgtttc gagatcgagg tggttggtcg gttcgtcgag
     3001 gagcaggacg tcgggattgc gcaggaggag gcgggcgagg aggatgcgca tgcgccagcc
     3061 gccggagaac tcctggcagg ggcggtcgag atcggcggtg cggaagccga ggccggcggc
     3121 gatgcggccg atgcgggagt ccatgtcgta ggcgcccagg cgctcgatct cgtggtgcag
     3181 atggccgaag cggtcgaggg cgcgcatgta atcgtcgctg gccgggtcga gctcgccgag
     3241 cttgtgggcc aggtcgtcgg cttcctgctg gtgactgagg acttcttcga agacgtggcg
     3301 gagttcgtcg cggaggcggc gattggagtg gagttcggcg tgctggggca ggtagccgat
     3361 ggtggcgtcg cgggggacgg tgaattcgcc ggcgtcgggg gagatctcgc cggcgcagat
     3421 gcgcagcagg gtggatttgc ccgagccatt ggcgccgacc agcgtggcgc attcgccggg
     3481 ctgaagcatg aagttgatgt gctcgagcag ggggcgggcg ccgatgaact tggagaggtt
     3541 gttgacggtc agcatggcgg gtggtcgctc ccaaaagccg gtattttagc cgaaggggcg
     3601 gggggcgcgc catgagaacg atgggggtat gcggtgggtg gggcggcgag ggcgggagag
     3661 gaagggggag gaccggcgaa ggcgttttcg agagccgaga gccgaattat ggagaaagag
     3721 gttggaggcg aggacgaaga cgatacttga gggaggccga tggccagggc gatagcaata
     3781 ccgattgcga taccgatcgt ggtggcgggg ggttgaagaa cggggcggga aggaccatga
     3841 tcgcgggtga tgaacggggt tgagccaaga gagcgcgtcc gcgtgctggt ggacgaccgc
     3901 gagaagacgc atgggctggt gccggcgatc gccgggctgg tgggcgaccg gttgacgctg
     3961 acgcgactgg tggcggggga tatccggatc ggggaggcgt atctgatcga gcggaagacg
     4021 gcggcggatt tcgtggcgtc gctgctggac gggcggttgg agcatcagct cgcggcgctg
     4081 gcgggagttc acccggctca ggcgctgctg atcatcgagg gtacgttcac tccggcggtg
     4141 ctggggggac tggcgccggc ggaggcgcgg cgggcgctgc tgtcgatcgc gctggactgg
     4201 cggattccgt gcctgcacgc cgccacggcg gaggagacgg cgcggtgggt ggcgctgctg
     4261 gtggagcggg tggagcggcc cggccggccg ttcgcggggg atttcgcagc ggcgcaagag
     4321 gtcgttccgc cgccgacggc gcggttctcg ggccgccgcg ccggcgcggg cgcgcacgcc
     4381 gtgcagcagg ccgcgttgcg gcggatcccg gggctggggg cgcgacgggc cgaggcgctg
     4441 ctgcggcggt tcgggtcgat cagcgcgctg gcgcgggcga cggaggcgga gattgcagcg
     4501 acgccgggga tcgggccgga gcgggcggcc gcggtgcgtc gggcgctggc gatgccgccg
     4561 ccgccatgag cccagtccgt gaaaactcca tcagccgatg gggagatcac aacacgggtt
     4621 ggaaggggcc ggagcgggcc agggaccg
//
LOCUS       DAOPCO010000313         4640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15411_length_4640_cov_4.829008, whole genome shotgun sequence.
ACCESSION   DAOPCO010000313 DAOPCO010000000
VERSION     DAOPCO010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15411_length_4640_cov_4.829008"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..770
                     /gene="aroQ"
                     /locus_tag="PLS90_15435"
                     /pseudo
     CDS             348..770
                     /gene="aroQ"
                     /locus_tag="PLS90_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
     gene            1700..3742
                     /locus_tag="PLS90_15440"
     CDS             1700..3742
                     /locus_tag="PLS90_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPK03840.1"
                     /translation="MHPNRPSAGIGPARLSCAGLLGLLLFVAACGEPPVLEPAQPTAT
                     EATTGTTAASVNTPTPEAAEPAGPPALLTTTAGTTEALILGVASLPADAPAEAQIAMD
                     ITPGRYGGQLVIPAVEGPRSFNPIFSSSITDQYVSGLQFSPLYDYDWYHQQDRPALAR
                     SWEFDKASLTWTFHLREGVKWSDGQPLTARDFLIYTELLFDPEIPNSERFFFQTGEGE
                     TAQRYEFAAPDELTFTVRIPEPDAFSFQNIGLIRALPRHVIEPVWRRGAFKEDFWSQG
                     VDPAELVVSGPFKLTSYRQGEALTFEPNPHYWRYDRQGQRLPYLERLVMLIVEDMEAM
                     NLRFLSGETDLLESIKPENLNQLQDQAQAGNFTIHELGPSLNTTHYFFNLHRGGTYLN
                     EKGERVEWEPERMGEEPPASLREFLPYLPARNARWFMDPEFRRACSEATNRQRMIDNI
                     LYGLGAPLYGPVPASNRQWHNPDIPRYAYNPDSARARLDALGFVDRDGDGFREDDEGH
                     PIRFSIVTNRGNDLRERVVQLLREDLRAVGLEVETRVLEFNNLITVLKETFDYDACLL
                     GLGTGVPPHPAMGGSLWRSDGEVHPGYPEQKAPQTEWEAELDRLYAAMKRTFDPEEQR
                     RIFFRMQEIYAEMQPSIHLFTQESHVATRNGLGNLKPTIIRSSVTHNVDELYWEAP"
     gene            3957..>4640
                     /gene="pgk"
                     /locus_tag="PLS90_15445"
     CDS             3957..>4640
                     /gene="pgk"
                     /locus_tag="PLS90_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPK03841.1"
                     /translation="MTILYNKKSVEDLEVNGKRVFVRCDLNVPIENGQVANDRRIKAS
                     VPTIEYLYQRGGIVILASHLGRPKGERKPEMSLRPVAARLTEILGRPVHFAEDCIGEA
                     TEQQVAALKPREIILLENLRFHKEEEKNDPEFSKKLARLADVYVNDAFGTAHRAHAST
                     EGITHHLSPCAAGFLIDKELKYLGNALAQPERPLLAILGGAKVGSKIGVITQLMKQVD
                     TLVIGGGMAY"
BASE COUNT          877 a         1313 c         1801 g          649 t
ORIGIN      
        1 gaattgaggc gaagccactc aggagagtgg cgctcccagg tttcggccgg ttgcttattt
       61 ccatgaggcg gaggggggga tggggggaga agggatcgat cacgaggacg aggacgaggg
      121 acggattcgg ctggggagcc tttgggggtg agaacccagg ctgagggacg agagacgagg
      181 gacgaatgcg tggggggcat ttgctggatg atttttgccg cgggattgcc taggatggac
      241 ccaaagcctt gggcggcggc aggccgggag acccccctgc gcgcgcgacg gcgccccgcc
      301 ggggcccgag tccctgctac gattctccgc tggaaagcga accttgcatc atgcgcattc
      361 tcgtgctgaa cggcccgaat ctgaacctgc tcggccaccg cgagcccgaa atctacggtc
      421 atcagacgct gcaggatctg gagaaccggt tgcgccggca ggcgcgcggt ctgtcgatcg
      481 acgtggaatt ttaccagagc aatcaggaag ggcagttgat cgacgcgctg caccgggcgc
      541 gcaaccgctg cgacggcgtg atcatcaacc cggggggtta cagccacacg tcggtggcgc
      601 tgcgcgacgc gatcgcgggg atcggcctgc cggtgattga agtgcatatc tccaacatcc
      661 acgcccgcga ggagttccgc agccggtcga tcacggcggg cgcctgcctg ggccagatca
      721 cggggctggg ccaggggggc tatgagctgg cgctcgaagc gctgttccac atctgccagg
      781 acatccggcg cgagtcgggc cgcggcgagg aacggcggga ggaacgccgc gaggagaagc
      841 gcgaggagaa acgcgaagag cggcgcgagg agaaggggga gggggatgat gacaaggggg
      901 accgcgaagc acggcgccgg cggcgcggtc ggcgcggcgg gcgcggacgc cggcgcgagg
      961 gcgagctgga ggagtcgcgc gagggccgcg aggagcgcga aggcgaacgc gaaacgtatg
     1021 atatcgccga gcgttactcg aacctggagg gggtgcgcgt gcggcgcggc ctggacgtga
     1081 tcgccgagga aggcgaggaa gatgaggcgc ttgagcagca ggaccaggac ctggtgagct
     1141 tccacgatca ggagacgctg gaggggatgg cgggccgatc cgaggcggag gggattgggc
     1201 gcgaggagtc gccgatggag atcgggccgg atgacgcggg ggacgagccg catccgacgc
     1261 cgggccggcg cagccggcgg gtggttcggg ggcggcaggc cgccggtccg gacgagctga
     1321 ccgacgccgc gatgatggac gacgggcggc ccgaggagcc gacggccgag gcgggcgagc
     1381 gggaagtggc ggaagaagtg gccgcgatcg cgcgcagcgt gagcggggaa ggcgatgagg
     1441 gcggcggcga ggcggacgca gccgaagggg aaccggccaa gcccgcgcgc aagacggcgc
     1501 gaaaaactac gcggaaggcg cccaagaagg ccgcgtcggg cgggacgcgc cggacaacgc
     1561 gcaagaaatc cgaggagtag gcaggggtcg ccgcgcctga cgcgggcgcg gcggccgcgc
     1621 accgtcgcgg gggttcgacc gcgcggcgca aacgggtttc cataagctgt cccatccgaa
     1681 ctgaagctgg agggagacga tgcatcccaa ccgcccctcg gccgggatcg gtccggcgcg
     1741 tctttcgtgc gcgggcctgc tcggcctgct cctgttcgtc gcagcgtgcg gggaaccgcc
     1801 ggtgctggag cccgcgcagc caaccgcgac ggaagcgacg acgggaacaa ccgccgcgag
     1861 cgtgaatacg ccgacgccgg aggcggcgga gcccgcgggg ccgccggccc tgctgacgac
     1921 gacggcgggc acgaccgagg cgctgatcct gggcgtggcc agcctgccgg cggacgcgcc
     1981 cgccgaagcg cagatcgcga tggatatcac gccggggcgg tacggcgggc aattggtgat
     2041 ccccgcggtg gaggggccgc gctcgttcaa tccgattttc tcgagcagca tcacggatca
     2101 gtatgtatcg ggcctccagt tctcgccgct gtatgactac gactggtatc accagcagga
     2161 ccggccggcg ctggccagga gctgggaatt tgacaaggcg agcctgacgt ggacgtttca
     2221 tctgcgcgag ggggtgaagt ggagcgacgg ccagccgctg acggcgcggg acttcctaat
     2281 ctacacggaa ctgctgttcg acccggagat ccccaacagc gagcgattct tcttccagac
     2341 gggagagggt gagaccgcgc aacgctatga gttcgcggcg ccggacgagc tgacgttcac
     2401 ggtgcggatc cccgagccgg acgcgttttc gttccagaac atcgggctga tccgcgcgct
     2461 gccgcgccac gtgatcgagc cggtgtggcg gcgcggcgcg ttcaaggagg atttctggtc
     2521 gcagggggtc gatccggccg aactggtcgt cagcggtccg ttcaaactga ccagctaccg
     2581 ccagggggag gcgttgacgt tcgaaccaaa cccgcactac tggcgctacg accggcaggg
     2641 gcagcggctg ccgtacctgg agcggctggt catgctgatc gtggaagaca tggaagcgat
     2701 gaacctgcgg ttcctgagcg gcgagaccga cctgctggag tcgatcaagc cggagaatct
     2761 gaatcagctt caggaccagg cgcaagcggg caatttcacg atccacgagc tggggccgag
     2821 tctcaatacg acgcactatt tcttcaacct gcaccgcggc gggacgtatc tgaacgagaa
     2881 gggggagcgg gtcgaatggg agccggagcg gatgggggag gagccgccgg cgagcctgcg
     2941 ggaatttctg ccgtatctgc cggcgcgcaa cgcgcggtgg ttcatggacc ccgagttccg
     3001 gcgggcctgc tcggaggcga cgaatcgcca gcggatgatc gacaacattc tatatgggct
     3061 gggggcgccg ctttacgggc cggttccggc gtcgaaccgc cagtggcata atccggatat
     3121 cccgcgctac gcgtacaatc cggattcggc gcgggcccgg ctggatgcgc tgggattcgt
     3181 cgatcgcgac ggcgacgggt tccgcgaaga cgatgagggg catccgatcc ggttttcgat
     3241 cgtgaccaac cgggggaacg atctgcggga acgggtcgtg cagttactgc gcgaggacct
     3301 gcgggccgtg gggctggaag tcgagacgcg ggtgctggag ttcaacaatc tgatcacggt
     3361 gctgaaggag acgttcgact acgacgcgtg cctgctgggg ctggggacgg gggtgccgcc
     3421 gcatccggcg atggggggca gtctgtggcg cagcgacggg gaggtgcatc caggctatcc
     3481 ggagcagaaa gcgccccaga ccgagtggga agccgagctg gaccggctgt atgccgcgat
     3541 gaagcggacg tttgacccgg aggagcagcg ccggatcttc ttccgaatgc aggaaatcta
     3601 cgccgaaatg cagccgtcga tccacctgtt cacgcaggag tcgcacgtgg cgacgcgcaa
     3661 tgggctgggg aacctgaagc cgacgatcat ccggtcgtcg gtgacgcata atgtggatga
     3721 gctttactgg gaggcgccgt gagagggaag ggaggaaatt aagggattcg agagccgagg
     3781 acgagggatg agttgggggc agccgggaaa gatcgttgcg ggctgaaaaa tgttgatttt
     3841 cgcccgcgca tacgagacat tgcggtgcta agtgcggccg ggcgattccc gcgcgagggg
     3901 acggcggccg agccggtagg ccggcaagcg gggcgagggc ccggggagta cgaacgatga
     3961 cgatcctgta caacaagaaa agcgtcgaag accttgaggt gaacggcaag cgcgtattcg
     4021 tgcgctgcga cctgaacgtg ccgatcgaaa acggccaggt ggccaacgac cggcggatca
     4081 aggcgtcggt cccgacgatc gagtatctgt atcagcgcgg cgggatcgtg atcctggcct
     4141 cgcacctggg gcggcccaag ggggagcgca agccggagat gagtctgcgg ccggtggcgg
     4201 cgcggctgac ggagatcctg ggccggccgg tgcatttcgc cgaggactgc atcggcgagg
     4261 cgacggagca gcaggtggcg gcgcttaaac cgcgcgagat catcctgctg gagaacctcc
     4321 gcttccataa ggaagaggag aagaacgacc cggaattcag caagaagctg gcgcggctgg
     4381 ccgacgtgta tgtgaacgat gcgttcggga cggcgcaccg ggcgcacgcc tccaccgagg
     4441 ggatcacgca ccacctgagc ccgtgcgccg ccgggttcct gatcgacaag gagctgaagt
     4501 atctgggcaa tgcgctggcg cagccggagc ggccgctgct ggcgatcctg ggcggggcga
     4561 aggtcggctc gaagatcggc gtgatcacgc aattgatgaa acaggtcgac acgctggtga
     4621 tcggcggcgg gatggcctac
//
LOCUS       DAOPCO010000314         4583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15629_length_4583_cov_5.201634, whole genome shotgun sequence.
ACCESSION   DAOPCO010000314 DAOPCO010000000
VERSION     DAOPCO010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_4583_cov_5.201634"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLS90_15450"
     CDS             <1..820
                     /locus_tag="PLS90_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPK03842.1"
                     /translation="DNQYREDELRHAELQTTLNGLSEQCEEKFERSLESLAEEVGEVE
                     KDAHGLHALVTELRAKIERIGPVNMAALDEYNEQSQRLEFLRAQHHDLTEARDQLRAT
                     IERLDETTRKLFHETFEAVRVHFIEMFRRLFNGGKADLILEAPEGLDPLLDGGIEILA
                     QPPGKKLQNIQLMSGGEKALTAIALLFALFLHKPSPFCILDEIDAPLDDINVERFKTV
                     LREFNEKTQFVIITHNKLTMELADALYGVTMEESGVSKLVSVRFEQAEQMVDVG"
     gene            1037..2464
                     /locus_tag="PLS90_15455"
     CDS             1037..2464
                     /locus_tag="PLS90_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03843.1"
                     /translation="MLAAGAGVGLMVWRGGSGNRAATPAARSADSVETVPVTAPTQPP
                     AYPEATPMTEMLLVPQDEIDATFRLELAVELDHLPAELILLPDSHPVSLVARLDQRRA
                     LPGGFRLENLRFVWSTNQGEVFEQTKTDEEGYYVSRAIFYPPKVSDFIELKAAAEIDL
                     IRDVAGREQRATSRIESALALLSPIDSVNMVNGRFGDYEVGEYLDPFDRSVASRFRVD
                     SDWYARYPERYMIPRVFYRADQRVKPMRISPSLELGLWMIDFPWKSLGATQYIALDYH
                     LVRKLEDLIALMRAAGYTISRLVAIYGFRPPAFNLGKIQSEAETTLKVPFSMHQYGRA
                     LDFIIDEDDDGLMDDLNGDGRCDVHDPAVIMHYVNALDRRYRAEGRMHMVGGAGLYHE
                     NDFLERNAHYHHNTPYIHVDTRGFLHDDGTLVRWPNTWPDGTTIRWGHIDEKGFASAG
                     ARIEPTPEPTADPHRPAIPLNFDEF"
     gene            2477..3700
                     /locus_tag="PLS90_15460"
     CDS             2477..3700
                     /locus_tag="PLS90_15460"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPK03844.1"
                     /translation="MRQPFLIALLAAWLWLGGGSVGAAEILPGVEVLARFYGDRLAGK
                     NVGIVTNPTGVNLDLVSTIDVVRAMPQVRVVRLFAPEHGLRGGLAAGEQVKGGRDPIS
                     GLPVISLHGATRKPAPAELEGIDVMLYDIQDVGHRTYTFISTLANVMEVCEDEGIEVM
                     VLDRPEPLGGLHFGGPMLDEEHRSFIGIHDIPQYYGLTPGEFSRLYQRERTPKLKLTV
                     IPMQGWRRGMTYGDLGWIWIPPSEHIPRWETSAFYAMTGTLGELRLVSEGVGTPLPFE
                     QIGAPWIDAAALAEALNAQGLAGVIFRPTTFRPRYGTHNGELCSGVQIHLRDAAAVEP
                     ARVSQAILRTLRRLYPERVRFGQPGEQAMFTKALGETAQIRALASGEYAAADEATEKR
                     LADYARRRQAVLIYE"
     gene            3700..>4583
                     /locus_tag="PLS90_15465"
     CDS             3700..>4583
                     /locus_tag="PLS90_15465"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK03845.1"
                     /translation="MAPRVLIIRLSALGDILHALPLLDALKRQWPDAFIGWLTEPAGA
                     GLLDGHPLIDRLHVVPRREWKANRWRALRGPLRALARDLRSQHYDTAIDAQGLTKSAL
                     WGLAARAPRRIGFAGRESRELAGLLNNERVLPAPDRLHVVERNLALLQPLGIQVRSNE
                     IHFPVHIPAAARTRADCILGEDEAIPLIVMNPGAGWATKIWPPECYGQLARRLLEERP
                     FRIALAWGPGEEPLVRAALAAAQAPPADFTLDTLPAGPGLSPLPATEFPELAAVIERA
                     TRLSVATPARRIWPRPSR"
BASE COUNT          784 a         1631 c         1423 g          745 t
ORIGIN      
        1 ggacaaccag taccgcgagg atgaattgcg ccacgccgag ctgcagacca cgctcaacgg
       61 cttgagcgaa cagtgcgagg agaaattcga gcgctcactg gaatcgctcg ccgaggaagt
      121 cggcgaggtg gaaaaggacg cccacggcct gcacgcgctg gtgaccgaac tgcgcgccaa
      181 aatcgaacgc atcggcccgg tcaatatggc ggcgctcgat gaatacaatg aacagtcaca
      241 gcgtctcgaa ttcctgcgcg cgcagcacca tgatctgacc gaggcccgcg atcagttgcg
      301 agcgacgatc gagcgcctcg atgagaccac gcgcaagctg ttccacgaaa cgttcgaggc
      361 ggtccgcgtc cacttcatcg agatgttccg ccggctgttc aatggcggca aggccgacct
      421 gatcctggag gcgcccgaag ggctcgatcc gctgctcgac ggcggcatcg aaatcctcgc
      481 ccagccgccg ggcaagaaac tccagaacat ccagctcatg tcgggcggcg aaaaggcgct
      541 caccgccatc gccctgctct ttgccctctt cctccacaag ccgtcgccgt tctgcatcct
      601 cgacgagatc gacgcgccgc tggacgacat caacgtcgag cggttcaaga ccgtcctgcg
      661 cgaattcaac gagaaaacgc agtttgtcat tatcacgcac aacaagctga caatggaact
      721 ggccgacgcg ctctatggcg tgacgatgga ggaatccggc gtgtcgaagc tcgtctcggt
      781 ccgcttcgag caagccgagc agatggtcga cgtgggctga cgccgcgacc ggcggcgcgg
      841 ggggaggcgg gctgcttcgc cgggctgttc tcgccgccgc ggcgatgcta tgctggaacg
      901 ccgcgcgtcc cgcgcccgcg cggaggacca cgatcggacc gtcactcaca ttccagatgg
      961 acccccaatg acgtttgtag acccggtgca gcggcgttcc gcaggcaatc tcagatttct
     1021 ggccggattg ctcctggtgt tggcggccgg ggcgggcgtg gggctgatgg tgtggcgcgg
     1081 gggctccggc aaccgggccg cgactcccgc cgcgcggtcc gccgactccg ttgaaaccgt
     1141 ccccgtcacc gcgccgactc agccgccggc ctatcccgag gcgaccccga tgaccgaaat
     1201 gctccttgtc ccccaggacg agatcgacgc cacgttccgg ctggagctgg ccgtggaact
     1261 cgatcacctc cccgcggagc tgatcctcct gcccgacagc caccccgttt cgctcgtcgc
     1321 ccggctcgac cagcggcgtg ccctgcccgg cggcttccgc ctggagaatc tccgctttgt
     1381 ctggtcgacg aatcaggggg aagtgtttga acaaaccaaa accgatgagg aaggctatta
     1441 cgtcagccgc gcgatcttct acccgccgaa ggtctccgac ttcatcgaac taaaggccgc
     1501 ggcggagatc gacctgattc gcgacgtcgc cggtcgggaa cagcgcgcca cctcgcggat
     1561 tgaatccgcc ctcgccctcc tttctcccat cgattcggtc aacatggtca acggccgctt
     1621 cggcgattac gaagtcggcg aatacctcga tccgttcgat cgctccgtcg cctcgcgctt
     1681 ccgcgtcgat tcggactggt acgcccggta tcccgagcgc tacatgatcc cccgcgtatt
     1741 ctaccgcgcc gatcagcgcg tcaaaccaat gcgcatctcc cctagcctcg aactggggct
     1801 gtggatgatc gacttcccct ggaagtcgct cggcgcgact cagtacatcg cgctcgatta
     1861 ccacctggtc cgcaaactcg aggatctgat cgcgctgatg cgcgccgccg gctacaccat
     1921 cagccgtctg gtcgcgatct acggcttccg gccgccggcc ttcaacctgg gcaagatcca
     1981 gagcgaggcc gaaaccacgc tcaaggttcc gttctccatg catcaatacg gccgggccct
     2041 tgatttcatc atcgacgagg acgacgatgg cctgatggac gacctcaatg gcgacggccg
     2101 gtgcgacgtc cacgatcccg ccgtaatcat gcattacgtc aacgcgctgg accgtcgcta
     2161 ccgcgccgag ggccgcatgc acatggtcgg cggcgcggga ctctaccacg agaacgactt
     2221 tctggaacgc aatgcccact accaccacaa caccccatat attcacgtcg acacccgcgg
     2281 cttcctccac gacgacggca ccctggtgcg ctggccgaac acctggcccg atggcacgac
     2341 gatccgctgg gggcatatcg atgaaaaggg attcgcttcg gcgggcgccc gcatcgagcc
     2401 gacgcccgag ccgaccgctg atccgcatcg cccggcaatc ccgctcaact tcgacgaatt
     2461 ctgaggaccc ttcccgatgc gacagccgtt cctgattgcg ctgttggccg cctggctgtg
     2521 gctcggcggc ggatcggtcg gcgcggccga aatcctgccc ggcgtcgagg tgctcgcgcg
     2581 gttctacgga gaccgcctcg ccggaaagaa cgtcggcatc gtcaccaatc ccaccggcgt
     2641 caacctggat ctggtttcga cgatcgatgt cgtgcgcgcc atgccgcagg tgcgcgtcgt
     2701 gcggctgttc gctccggagc atggcctgcg cggcggcctg gcggccggcg agcaggtcaa
     2761 gggcggccgc gacccgatca gcggcctgcc cgtgatcagc ctgcatgggg ccacgcgcaa
     2821 gcccgcgccc gccgagctcg agggaatcga cgtcatgctc tacgacatcc aggacgtcgg
     2881 ccaccgcacc tatacgttca tctccaccct ggccaacgtc atggaagtct gcgaggacga
     2941 agggatcgag gtgatggtgc tcgaccgccc cgaaccgctc ggcggcctcc acttcggcgg
     3001 accgatgctc gacgaggagc accgttcgtt tatcggcatc cacgatatcc cgcagtacta
     3061 tggcctgacc ccgggcgagt tctcccggct ctaccagcgc gaacgcacgc cgaaattgaa
     3121 gctgacggtc atcccgatgc agggctggcg ccgcggcatg acctacggcg atctgggttg
     3181 gatctggatt cccccctcgg aacacatccc gcgctgggaa acttccgcgt tctacgcgat
     3241 gaccggcacg ctcggtgaat tgcgcctggt cagcgaaggc gtcggcaccc ccctcccatt
     3301 cgaacagatc ggcgcgccgt ggatcgatgc ggccgcgctc gccgaagcgc tcaacgccca
     3361 ggggctggcg ggggtcatct tccggcccac cacgttccgg ccccgttacg gcacgcacaa
     3421 cggcgagctg tgcagcggcg tgcagattca cctgcgcgat gcggccgccg tcgagccggc
     3481 gcgcgtcagc caggccattc tgcggacgtt gcggcggctc tatccggaac gggtccgttt
     3541 cggccagccg ggcgagcagg caatgttcac caaggccctc ggcgaaaccg cgcagattcg
     3601 cgcgctggcc agcggcgaat acgccgcggc ggatgaggca accgaaaaac gcctggccga
     3661 ctacgcgcgc agacgccagg cggtcctgat ttacgagtaa tggcgccgcg cgtgctgatc
     3721 atccggctct cggcgctggg cgacatcctc cacgccctgc cgctgctcga cgcactcaaa
     3781 cgacaatggc ccgatgcctt catcggctgg ctgactgagc ccgcgggggc ggggctgctc
     3841 gacggccacc cgctcatcga ccgcctgcac gtcgtgcccc gccgggagtg gaaggccaac
     3901 cgctggcggg cgctgcgcgg gccgctgcgc gccctcgccc gcgacctccg ttcccagcat
     3961 tacgatacgg cgatcgacgc ccaggggctg accaagagcg cgctctgggg cctcgccgcg
     4021 cgagccccgc ggcggatcgg cttcgcgggc cgcgaatcgc gtgaactggc gggcctgctc
     4081 aataacgaac gcgtcctgcc cgcgcccgac cgcttgcatg tcgtcgagcg caatctggcg
     4141 ctcctccagc cgctcggcat tcaagtccgg tccaatgaga tccatttccc ggtccatatc
     4201 ccggcggccg ctcgcacccg tgccgactgc atcctgggcg aggatgaagc aatcccgctg
     4261 atcgtgatga atcccggcgc cggctgggcc accaagatct ggccgcccga atgctacggt
     4321 caactcgccc gccgcctgct ggaggaacgg ccgttccgga tcgcgctggc ctggggcccc
     4381 ggcgaggaac cgctcgtgcg cgccgccctg gcggcggctc aggccccccc ggctgacttc
     4441 accctcgata cgcttccggc cggccccggc ctcagcccgt tgcccgcgac ggagttcccc
     4501 gagctggccg ccgtgatcga acgggcgacc cgtttgtcgg tggcgacacc ggcccgacgc
     4561 atctggccgc ggccctcgcg aag
//
LOCUS       DAOPCO010000315         4559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15726_length_4559_cov_4.685835, whole genome shotgun sequence.
ACCESSION   DAOPCO010000315 DAOPCO010000000
VERSION     DAOPCO010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15726_length_4559_cov_4.685835"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLS90_15470"
     CDS             <1..1145
                     /locus_tag="PLS90_15470"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPK03846.1"
                     /translation="LADDLDVGAAAAAVGEVVAVERRAWELDRADGTAVAAADLDGGR
                     IWGRGAYDCKMLAAAQLFVMAVLAEEGLPRGGRLRLVMAADEEAGGRQGFRWLAEVRP
                     DFLRCDVALCEGGGASLGDWNGESCFTIGAGEKGRCEVQVAFRAPGGHASTPWGRRNP
                     IRDAAEFIRRLGDTPVEVRPASPVFQTVAQWLRLSEPLSPANLDWAIGQIEQRSPALA
                     RSLRAQSRLSFAPTIIRGGDKSNSLPDEVVVTCDFRLLPGDDEALVEQVVQRCLEGIE
                     AARVRIDWTSEASHSPLDAGLLARFEAAASRAAGRPMRGAPVWCAGFTDARLVRPLGT
                     PVYGFQFIEPNADPERLGIHCVDESIEVGMLLPCAVALASFILDTL"
     gene            complement(1197..2027)
                     /locus_tag="PLS90_15475"
     CDS             complement(1197..2027)
                     /locus_tag="PLS90_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPK03847.1"
                     /translation="MEFFQSLAQHAHMQHALLAGLLASIAAGVVGTYVVVRRIVFISG
                     GIAHAVLAGMGIAYWMEGDPFVGALIAAIVSALIIGLISLRAGQHEDTLIGALWAVGM
                     AVGILFISRAPGYKVDLNSYLFGDILLVTRQDLLLIGVLDLAIVLAVIYFYREFQAVC
                     FDEEYARLQGVPADLVYLLLLCLIALTVVTMIRLVGLILVIALLTLPAAIAQQYLRSL
                     GRMMILGAILAFGFTFAGLAASYQPDLPAGSTIVLVAATAYLVSVAGRMIARRWRERS
                     "
     gene            complement(2139..2492)
                     /locus_tag="PLS90_15480"
     CDS             complement(2139..2492)
                     /locus_tag="PLS90_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HPK03848.1"
                     /translation="MRVGKCEVLGVEIGARGTCAHYNSERDIVAIRFRCCGEYYGCHA
                     CHALLAGHPAERWPHFEFDRRAILCGACGEELTIHQYLSSPEACPLCHAPFNPGCRKH
                     HQLYFEVQGGPRLGS"
     gene            2626..3858
                     /locus_tag="PLS90_15485"
     CDS             2626..3858
                     /locus_tag="PLS90_15485"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPK03849.1"
                     /translation="MKHVIILGDGMADYPVERLGGRTPLQVANKPHIDRIARDGRTGM
                     LRTLTPDLPTGSAVANLAVLGYDPRACFQGRGVLEAASMGVPIGEDQIALRCNLICTE
                     NGNIKNHSAGHISSAEAAELIRALDQQLGGDTLRFHPGVSYRHLLVATGLNPDLACAP
                     PHDHPGEPVEELMIKPRDPAAEATARRLNDLTRQSWSILADHPVNRRRRSAGKDPANS
                     IWLWSPGRKPRMWTFHDRFGIRGAVISAVDLVRGIGVYGGLEIIDVDGATGLWDTNYE
                     GKADACLAALERVDFVYLHVEAPDEAGHEGDLELKIRTIEDIDLRLVGRILEGLERQA
                     IDAVVAILPDHPTPVELRVHVRDPIPFAILDPRQAPDEVQAYDEVSCRSGGYGTLEGD
                     AFIRAVFGQTDEQEPQTP"
     gene            4012..4359
                     /locus_tag="PLS90_15490"
     CDS             4012..4359
                     /locus_tag="PLS90_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03850.1"
                     /translation="MPTPVISIVLFLVAALLGAVGQYLYKSGADAVTGGWSGYVLNLR
                     LLAGVVCYVAVMVLFVAAFRKGGSLAVLYPLYASTFIWGALIALWAYGTPIRGVNVAG
                     MALLVGGMYLMGR"
     gene            4364..>4559
                     /locus_tag="PLS90_15495"
     CDS             4364..>4559
                     /locus_tag="PLS90_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPK03851.1"
                     /translation="MDRRPLKTRDKAASHAVAGWLAARGVTPNAISWSSLVFGIAAGA
                     AFAATAHLPEGARLLWFLGAL"
BASE COUNT          731 a         1694 c         1430 g          704 t
ORIGIN      
        1 ggctggctga tgacctcgat gtcggtgcgg cagccgctgc agttggcgag gtcgtcgccg
       61 tggagcgtcg tgcctgggag ctcgaccgtg cagacggaac cgctgtggcc gccgcggatc
      121 tcgacggcgg gcgcatctgg ggccgcggcg cctacgactg caagatgctg gccgccgccc
      181 agttgttcgt catggccgtc ctggccgagg agggccttcc ccgcggcggc cggctgcgtc
      241 tggtcatggc ggccgacgaa gaagccggcg gccgccaggg gttccgctgg ctcgccgagg
      301 tccggcccga ctttctccgc tgcgacgtcg ccctgtgcga ggggggcggc gccagcctgg
      361 gcgactggaa cggcgaatcc tgcttcacga tcggcgccgg cgagaaaggc cgctgcgaag
      421 tgcaggtcgc cttccgcgcc cccggcggcc acgccagcac cccctggggc cgccgcaacc
      481 ccatccgcga cgccgccgag ttcatccggc ggctgggaga caccccggtc gaggtccggc
      541 cggcatcgcc cgttttccag accgttgccc aatggctcag gctgtctgaa ccgctgtcgc
      601 ccgcgaatct ggactgggcg atcggccaga tcgaacagcg ctcgccggcg ctggcccgtt
      661 cgctccgcgc gcagagccga ctgtcgttcg cgcccacgat catccgcggc ggggacaaga
      721 gcaactcgct gcccgacgag gtcgtcgtca cctgcgattt tcggctgctg cccggcgacg
      781 atgaagccct ggtcgagcag gtcgtccagc gttgcctgga ggggatcgaa gcggcgcgcg
      841 tccggatcga ctggaccagc gaggccagtc attccccgct cgacgccggc cttctcgcgc
      901 gttttgaggc ggcggcctcc cgcgccgccg gccgtccgat gcgcggcgcg ccggtctggt
      961 gcgccgggtt caccgacgcg cgcctggtgc gcccgctcgg cacccccgtc tacggctttc
     1021 aattcattga acccaacgcc gatcctgaac gcctgggcat ccactgcgtt gatgaatcga
     1081 tcgaggtcgg catgctcctg ccctgcgccg tcgccctcgc cagcttcatc ctggacacgc
     1141 tctgatcgtg cttccgggga cgctgtgagg cgaagccgcg ctttccagct tgcccgctaa
     1201 ctccgctccc gccaacgccg tgcgatcatg cggcccgcga ccgaaaccag gtaggccgtc
     1261 gcggccacca gcacgatcgt cgaccccgcc ggcaggtccg gctgatacga cgccgccagc
     1321 cccgcaaacg tgaatccgaa agccagaatc gcccccagga tcatcatgcg gcccagcgac
     1381 cgcagatact gctgcgcgat cgccgccggc agcgtcagca gcgcgatcac caggatcagg
     1441 cccaccaggc gaatcatcgt gacgaccgtc agcgcgatca ggcagagcag cagcaggtaa
     1501 accaggtccg ccggcacccc ctgcagccgg gcatattcct cgtcgaaaca gaccgcctga
     1561 aactcccggt agaagtaaat gaccgccagt acgatcgcca ggtccagcac cccgatcagc
     1621 agcagatcct gccgcgtcac cagcaggatg tcgccgaaca gatagctgtt caggtccacc
     1681 ttgtagcccg gcgcccgcga gatgaacagg atcccgaccg ccatccccac cgcccagagc
     1741 gccccgatca gcgtgtcctc gtgctggccc gcgcgcaggc tgatcagccc gatgatcagc
     1801 gccgagacga tcgcggcgat cagcgcgccg acgaacggat cgccctccat ccagtaggcg
     1861 atgcccatgc ccgccagcac cgcatgcgcg atccccccgc tgatgaagac gatccgccgc
     1921 accacgacgt aggtgccgac cacgcccgcc gcgatcgacg ccagcaggcc cgccagcagc
     1981 gcgtgctgca tgtgagcgtg ctgggcgagg ctctggaaga attccatcat ggcgctcggc
     2041 ggggccgggg gtcaggggcg caaagttccc ccgccatcgt agcgaccgcg ccgcgccgcg
     2101 caacaacatt gagcgcaacc ctcccgcccg gccgccccct atgaacccag acgcggcccc
     2161 ccctggactt caaaatagag ctgatgatgc ttgcggcaac ccgggttgaa gggcgcgtgg
     2221 cacagcgggc acgcctcggg cgagctgaga tactggtgaa tcgtcaattc ctcgccgcac
     2281 gcgccgcaca ggatcgcgcg ccggtcgaac tcgaaatgcg gccagcgttc ggccggatgc
     2341 cccgccagca gggcatggca cgcgtggcag ccataatact cgccgcagca gcgaaaccgg
     2401 atcgccacga tgtcgcgctc cgaattgtaa tgcgcgcacg tcccacgcgc gccgatctcg
     2461 acccccagca cttcgcactt gcccacccgc accggcgcgc cctccttcgt atcccaggaa
     2521 tcgccgtttc aactcgagaa atcaggccgc cattgagtcc ggccgccgtt ctggtgtatc
     2581 accataccgt cacggctgac gactcacccc gcgaaagatc acgcgatgaa acatgttatc
     2641 atcctcggcg acggcatggc cgattacccg gtcgaacggc tcggcggccg caccccgctc
     2701 caggtcgcca acaaacccca tatcgaccgg atcgcccgcg acggccgtac gggcatgctc
     2761 cgcacactga cccccgatct gcccaccggc agcgccgtcg ccaacctggc cgtcctcggc
     2821 tatgaccccc gcgcctgctt ccaggggcgc ggcgttctgg aggccgccag tatgggcgtg
     2881 ccgatcggcg aagaccagat cgccctgcgc tgcaacctga tctgcaccga aaacggcaac
     2941 atcaagaacc attccgccgg gcatatctcg tcggccgagg ccgccgaact gatccgcgcc
     3001 ctggatcagc aactcggcgg cgacacgctg cggttccatc ccggcgtcag ctaccgccat
     3061 ctgcttgtgg ccacggggct gaaccccgac ctggcctgcg cgccaccgca cgatcatccc
     3121 ggcgaacccg tcgaggaact gatgatcaag cccagggacc cggccgccga agcaaccgcc
     3181 cgccgcctca acgacctgac gcggcaatca tggtccatcc tcgccgacca ccccgtcaac
     3241 cgccggcgcc ggtcggcggg caaggacccc gccaattcga tctggctctg gtcgcccggc
     3301 cgcaagcccc ggatgtggac gttccacgac cgattcggca tccgcggcgc cgtcatctcc
     3361 gccgtcgacc tcgtgcgggg catcggcgtc tacggcgggc tcgaaatcat cgacgtcgac
     3421 ggcgccaccg gcctgtggga caccaactac gaaggcaagg ccgatgcctg cctggcggcc
     3481 ctcgaacgcg tcgacttcgt ttacctgcac gtggaggccc ccgatgaggc cggccatgag
     3541 ggcgatctgg aactcaagat ccggacgatc gaggacatcg acctgcgctt ggtgggacgc
     3601 atcctggagg gcctggagcg ccaggcgatc gacgccgtcg tcgcgatcct ccccgatcac
     3661 cccacccccg tggagctgcg ggttcacgtg cgggatccga tcccattcgc catcctcgat
     3721 ccgcgccagg cgcccgacga agtgcaggcc tacgatgagg tcagttgccg ctcgggcggc
     3781 tacggcacgc tcgaaggcga tgcgttcatc cgcgccgtct tcggccagac cgacgagcaa
     3841 gaaccccaaa ccccctgagg ccagggacgc gttcgctctt caacgaaaac gccgtgacgg
     3901 attttggcgt cagggtcgtg agtttccatt gcgtcgctcc gccgcgcgac cttatcctgt
     3961 ctgcggttgt cccggttcaa cactgatctc caggcgcgcg ggagaagacc catgccgacg
     4021 ccggtcatct cgatcgtgct gttcctcgtc gccgcccttc tgggggcagt ggggcagtat
     4081 ctctataaat cgggcgccga cgccgtcacc ggcggctggt ccggctacgt gctcaacctc
     4141 cgcctgctgg cgggcgtggt ctgctacgtc gccgtcatgg tgctcttcgt cgcggccttc
     4201 cgcaaaggcg gttcgctcgc cgtgctctac ccgctctatg cctccacctt catctggggc
     4261 gcgctgatcg ccttgtgggc gtatggcacg cccatccgcg gcgtcaacgt cgccggcatg
     4321 gcgctgctgg tcggcggcat gtatctgatg ggcaggtagc gcgatggacc gccggccgct
     4381 caagacgcgc gacaaggccg cctcgcacgc ggtcgccggc tggcttgccg cgcgcggcgt
     4441 cacgccgaac gccatttcct ggtccagcct ggtcttcggc atcgccgccg gggccgcgtt
     4501 cgccgccacc gcccacctgc ccgagggggc gcgtctgctg tggtttctcg gtgcgctgt
//
LOCUS       DAOPCO010000316         4557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15735_length_4557_cov_5.083963, whole genome shotgun sequence.
ACCESSION   DAOPCO010000316 DAOPCO010000000
VERSION     DAOPCO010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15735_length_4557_cov_5.083963"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /gene="gpmI"
                     /locus_tag="PLS90_15500"
     CDS             complement(<1..1456)
                     /gene="gpmI"
                     /locus_tag="PLS90_15500"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPK03852.1"
                     /translation="MAPKLRKRPVVLIVRDGWGHNPFAEWDAANAVHLARRPVDRRLL
                     EEYPHVQIHTSGEDVGLPAGVMGNSEVGHQNIGAGRIVNQEIMRITQTIRDGSFFENP
                     VLLGAFEHARRHNGSVHLLGLFSDGRVHSDMGHAEAVVELARRVDFPADRFFVHVITD
                     GRDTPPQSGKGFVAQFQRKLRESGAGRIASVVGRYYAMDRDNRWDRVQQAYEMLISGA
                     PRVVEDALAGIEHYYQNPTESNRTGDEFITPLSVAPDGRIDPRQTVRDGDAVIFFNYR
                     GDRPREICKAFVLEDDAWAGVPNGGFDRGQRPRNLYFVGMTRYEQGLPIEVAFDKPPK
                     LVNILGEWAAKQGLRQFRSAETEKFPHVTFFFNDYREAPFEGEERQIVPSPRDVKTYD
                     QKPEMSAYEVTAEVLQRIEEDAIDLYIINFANGDMVGHTGVLAAAIKAVETVDECVGR
                     IVEATLGKGGALVVTADHGNCEQMIDPETGGPHTA"
     gene            complement(2123..2827)
                     /locus_tag="PLS90_15505"
     CDS             complement(2123..2827)
                     /locus_tag="PLS90_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPK03853.1"
                     /translation="MHLPRLHDRRFRSRPVRALILDMDGTIVDTRAFHMRSWRVLVGE
                     LGLGEREYQIAEAGFGKTNWMIFSQWWPDQRPRGVDDDELSERKESIFRELIRGHVVP
                     RPGFRELIELALRNGIKLGLATSSPKENALFLLEELGVRELFGAVTWGWAGIRSKPHP
                     DSFLLAARLLEVPPVNCLGYEDSDHGLLALRRAGMGIVGIAERPLDLNRLRLWTPYVF
                     SDFQGATWMLRRVMEG"
     gene            2965..3258
                     /locus_tag="PLS90_15510"
     CDS             2965..3258
                     /locus_tag="PLS90_15510"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK03854.1"
                     /translation="MSPLVVADFNDDGRPDVAVVHSENEAGRILLILNHQGDLDPAGA
                     LMIDEHGPRAGTLAAGDLDGDGLPDLVVSHPAGISYYLNQIRHNAARRWTAYD"
     gene            complement(3307..>4557)
                     /locus_tag="PLS90_15515"
     CDS             complement(3307..>4557)
                     /locus_tag="PLS90_15515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03855.1"
                     /translation="ARRRAVRALLAAALVLIPAALIVCFSAGSNLGLVCGALVLLAGR
                     LRRGGAGWFVRRRGLWLALVAAWAALIVFFCTDHPLNPHRPGILAQGFGSARWQAGGP
                     TRLAIWAQSLEMIRQRSLLGFGPGNFTYVFPEMDSALIADRPDLLVYQGMWTNAAHNV
                     ILQMWTELGVVGLFLFLTLVCLALYQLLKGLPDADEEGATIRLALLALLTAWLVQGQV
                     NFVSVHPSGLLTFYVLLAAVIAEKRARPRAGGAMPSLRYETGPLALQLDWHSMKKPTG
                     LGLAVLLPDGAAIALSLLLVCVVLFWAPRRVAPVLAQRAYRAAVQASQAGAAGSAEEH
                     FRRALRLDPDAQDVRSRFSVFLLEQNRPEEALAEIARVRERLNSNELWAREAQALAAL
                     GREQEAVEHYETYLARVWSARNPR"
BASE COUNT          799 a         1752 c         1318 g          688 t
ORIGIN      
        1 gcgccgtgtg cggacccccc gtctccgggt cgatcatctg ctcgcagttg ccatggtcgg
       61 ccgtcacgac cagcgcgccc cccttgccca gcgtcgcctc gacgatccgg ccgacgcact
      121 cgtccactgt ctccaccgcc ttgatcgccg ccgccagcac gcccgtgtgt ccaaccatgt
      181 cgccgttggc gaaattgatg atatacagat cgatcgcgtc ctcctcgatc cgttggagca
      241 cctccgccgt cacctcgtac gccgacatct ccggtttctg gtcgtaggtc ttcacgtcgc
      301 gcggcgaggg gacgatctgc cgctcctccc cctcaaacgg cgcctcccgg taatcgttga
      361 agaagaacgt cacatgcggg aatttctcgg tctccgccga ccggaattgc cgcagcccct
      421 gcttggccgc ccattccccc aggatgttga ccagcttcgg cggtttgtcg aacgccacct
      481 cgatcggcag accctgctcg tagcgcgtca tcccgacgaa atacaggttc cgcgggcgct
      541 ggccgcgatc gaatccgccg ttgggcacgc cggcccaggc gtcgtcctcc agcacgaacg
      601 ccttgcagat ctcgcgcggc cggtcgcccc ggtagttgaa gaagatgacc gcgtcgccgt
      661 cccgcaccgt ctgccgcggg tcgatccggc cgtcgggcgc cacgctcagc ggcgtgatga
      721 attcatcccc cgtccgattg gattcggtcg gattctgata atagtgctcg atcccggcca
      781 gcgcgtcctc caccacgcgc ggcgcccccg agatcagcat ctcgtaggcc tgctgcaccc
      841 gatcccagcg gttgtcccgg tccatcgcat agtagcgccc gaccacgctc gcaatccggc
      901 ccgccccgct ctcgcgcagc ttgcgctgga actgcgcgac gaaccccttg ccgctctggg
      961 gcggcgtgtc gcggccgtcg gtgatcacgt gcacgaaaaa ccggtcggcc gggaaatcca
     1021 ccctccgcgc cagctccacc accgcttcgg cgtgccccat gtcgctgtgg acccggccgt
     1081 cgctgaacag cccgagcaga tggaccgacc cgttgtgccg gcgcgcatgt tcgaacgccc
     1141 ccagcagcac cgggttttcg aagaacgagc catcgcgaat cgtctgcgtg atgcgcatga
     1201 tctcctgatt cacgatccgg ccggcgccga tgttctggtg ccccacctcg ctgttgccca
     1261 tcacccccgc cggcaggccg acgtcctcgc ccgacgtgtg gatctgcacg tggggatact
     1321 cctccagcag ccggcggtcc accggccggc gcgccagatg gacggcgttg gccgcgtccc
     1381 actcggcgaa cgggttgtgt ccccagccat cccgcacgat caggacgact ggacgcttcc
     1441 tcaacttcgg ggccatcttg ctcactcatt tcatcaggat tcgaagccgg cgggcatcca
     1501 tcccctcccc ctgcccggct cagagacatt caaccccaca tcctatcaag cccgcttcgg
     1561 ctcggcatcg tctttttcgc cgccccgagg cgcttgagtg gagcgggcgc ggcacgcgaa
     1621 ccacgcaacg gacgcgagaa acacaagcgg ttgccccgaa ctcgcctctc gcctcgcggc
     1681 cctcgtcccc ggctctcgca aaccaccagt tccgtcccga gttgtgaacc gcgggttgat
     1741 gcaatcgatc gtgcgatgct cctcaaatcc agggcggatc atcaggaacg catcggttga
     1801 acaccagatc ccccgcatcc cggagggatg ccagagtcta gccggtggtc gagcgcagcg
     1861 ataccaccgg tcccccggac cgcatccccc cgcatcccgc agggatgcca gattccagcc
     1921 tgtcgaactc gcacctcccg ccgccatcgc taacctcccg agtccgcgga gcggacgacg
     1981 agaataaagc gcagggtgac ggccgcaggc cggaaccctg cgtcatcgcc caattcaatc
     2041 aaacccctga aaggggtggc cggatagcgc ccgaaacgca aacccccgcc gtggtcgcct
     2101 tgccctcgcc ggtcaccccc atttacccct ccatcacccg ccggagcatc catgtcgccc
     2161 cctgaaaatc cgaaaacaca tacggcgtcc acagccgcag ccggttcaga tccagcggcc
     2221 gttccgcaat cccgacgatc cccatccccg cccgccgcaa cgccagcagc ccatggtcgg
     2281 aatcctcgta ccccaggcag ttgaccggcg gcacttccag cagccgcgcc gccagcagga
     2341 acgaatcggg atgcggtttg ctgcggatgc cggcccaacc ccacgtgacc gcgccgaaca
     2401 gctcgcgcac ccccagttcc tccagcagaa acagtgcgtt ctccttcggg ctcgacgtgg
     2461 ccaggcccag cttgatcccg ttgcgcagcg ccagctcgat cagctcccgg aatcccggac
     2521 gcgggacgac gtgcccgcgg atcagttctc gaaaaatcga ctccttgcgc tccgacagtt
     2581 cgtcgtcatc gaccccccgc ggccgttggt ccggccacca ctggctgaag atcatccagt
     2641 tggtcttgcc gaaaccggcc tcggcgatct gatattcgcg ttcgcccaac cccagttccc
     2701 ccaccagcac gcgccacgag cgcatgtgga acgcgcgcgt atcgacgatc gtgccgtcca
     2761 tgtcgagaat caacgcgcgg acgggacgcg agcgaaaacg tcggtcgtgc aggcggggca
     2821 gatgcatcgg tgaaatccgg ccatccagga tgggatcgcg acgccgaagg cgcacgctcc
     2881 cgtcttatat cagatcgcga ccggattcgg caccagcgat tccgacacaa aatatcccca
     2941 tgtccgccgc acctgccgtc gttcttgagc ccgcttgtcg tcgcggattt caatgatgat
     3001 ggacgaccgg acgtcgcggt ggttcacagt gagaacgagg cgggacgaat cctgctcatt
     3061 ctcaaccatc agggagacct cgatccggcc ggggccttga tgatcgacga acacggcccg
     3121 cgcgcgggaa cattggcggc cggcgacttg gacggcgatg gcctgcccga cctggtggta
     3181 tcccaccccg cgggcatcag ctactatctg aatcagatcc gccacaacgc cgcgcgacgt
     3241 tggaccgcct acgattgacc gcgcaacggt accgcggagc acgcggctcc gccgcacgca
     3301 ccaaggtcac ctcgggttgc gcgccgacca cacgcgcgcg agatacgtct cgtagtgctc
     3361 gacagcttcc tgctcgcggc ccagcgcggc cagcgcctgc gcttcgcgcg cccacaattc
     3421 attggaattg agtcgctcgc gcacccgggc gatctctgcc agcgcctcct ccggccgatt
     3481 ctgctccagc agaaacacgc tgaaccggct gcggacgtcc tgcgcgtccg gatcaagccg
     3541 cagcgcgcgc cggaagtgct cctcggccga cccggccgcg cccgcctggc tcgcctgcac
     3601 ggcggcgcga tacgcccgct gcgccagcac cggcgccacc cgccgcggcg cccaaaacag
     3661 gaccacgcag accagcagca ggctcagcgc gatcgccgcg ccgtccggca gcagcaccgc
     3721 caggccaagg cccgtgggtt tcttcatgct gtgccagtca agctgcaacg ccagcggccc
     3781 ggtttcgtag cgcagcgacg gcatggcgcc ccccgcgcgc ggccgcgccc gcttctccgc
     3841 gatcaccgcc gccagcagca cgtaaaacgt caacaggccg gacgggtgca ccgacacgaa
     3901 attgacctgc ccctgcacga gccacgccgt cagcagcgcc agcagcgcca gccggatcgt
     3961 cgccccctcc tcgtcggcgt cgggcaagcc cttcaacagc tgatacagcg ccaggcagac
     4021 cagcgtcaga aacagaaaca gccccacgac gcccagttcg gtccacatct gcaggatcac
     4081 gttgtgcgcc gcgttggtcc acatcccctg gtagaccagc aggtcgggcc ggtcggcgat
     4141 cagcgccgaa tccatctcgg gaaagacgta agtgaaattg cccggcccga accccagcag
     4201 cgagcgctgg cggatcatct cgagcgactg cgcccagatc gcaagccgcg tcggcccgcc
     4261 cgcctgccag cgcgccgagc caaaaccctg cgccaggatc cccggccggt gcgggttaag
     4321 cggatgatcc gtgcagaaga aaacgatcag cgcggcccac gccgccacca gcgccagcca
     4381 cagcccgcgc cgccggacga accaccccgc gccgccgcgc cgcagccgcc ccgcgagcag
     4441 gaccagcgcc ccgcacacca accccagatt cgacccggcc gaaaagcaga cgatcagcgc
     4501 ggcggggatc agcaccagcg ccgccgccag cagcgcccgg accgcccggc gccgcgc
//
LOCUS       DAOPCO010000317         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15761_length_4552_cov_4.497443, whole genome shotgun sequence.
ACCESSION   DAOPCO010000317 DAOPCO010000000
VERSION     DAOPCO010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15761_length_4552_cov_4.497443"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLS90_15520"
     CDS             <1..525
                     /locus_tag="PLS90_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03856.1"
                     /translation="FEEVLTAEHLLKSIGAAPAGIGATGMYPEGIDGREDVQRLLMER
                     GYHWVSSRFNLKRTLHEMQPYRYPNGLLELPCAGWSDFTWFYASDQPTLPEYDVGGSP
                     TLEQFIRHVQDLTTLAARDGLILAVCLHPGLLAAHDPELHCVPRVIQHARRLGVEPAD
                     LRTIAAACPPARGG"
     gene            609..1556
                     /locus_tag="PLS90_15525"
     CDS             609..1556
                     /locus_tag="PLS90_15525"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HPK03857.1"
                     /translation="MTLVHEGTGLLHGVFPILATCFKPDDSIDWESQQRLIDFCIEGG
                     VHGLVILANASEGHLLSEQEKRELIVFCLKQIAGRVPVIATVNHPSAKVAAELARHAE
                     QHGAAAVMALPPFFGRWRSGQDEIFHFFSTLNAAVRIPIVIQDHVLSDITLPVAFLIE
                     LGKKLEHVRYVKLEAGNILHKAWKIATTPQQPFLGIFGGNSGAFMPEEVEAGCTGTMP
                     ACYMPEVFRTAWDMVAAGAGDAAVAYFTPFSRLASYEKDVANRCVWKEILVARGIIQH
                     NHVREPAPGYADPWQRQQLLRLAGRMGLLAGAAAGGKSQ"
     gene            1553..2761
                     /locus_tag="PLS90_15530"
     CDS             1553..2761
                     /locus_tag="PLS90_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPK03858.1"
                     /translation="MSLVIEKIDVYILKLDKHYQIRGDMDAPELFPGTDYYLESSLRQ
                     VSSLKTETCLIKLTTNRGLEGWGEAQAPIVPETAASIITRLLGPFVIGKDPLRNEVLV
                     DGMSHLMEVRGHGSGFMLDAIAAIDLALWDLKGKYYGAPIFELLGGPYRTRLPAYVSG
                     LRQPTLAEQCVAAREYRDQGIQGIKLFLGQGVEADVHTMRTIREAVGPAFRLQTDIMW
                     KYGLAESKRLAREMEAVNMEWMEAPMAPYDLEGHRELARSTSVPLAIGETFRAVHEFM
                     PWFKAQALAIAQPDIMRIGLTVGCKIAALALAFDKQLAPHVGVCTSIGTAAAWHFSAS
                     LSNFLIQEWQAQLFDSTNALLRKPLSVEGGYLVVPSDPGLGIEVDEAAVRRHAADHWE
                     ITASGIHHAA"
     gene            2751..3536
                     /locus_tag="PLS90_15535"
     CDS             2751..3536
                     /locus_tag="PLS90_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPK03859.1"
                     /translation="MLLEHGLLGVGSFAIGLVVGSTSLGGFLLVPLMLMQAGMTLSTS
                     VLISLLSFVPSGMVNTYQYACRKCVDVKLFALLLVSSLPGSYLGVYWSHAVSATASRR
                     LLGCFLLVTGAFLCLRLLGEIKRRGDGAATSERVQLAGKPGRILLFVALGVMAGLSST
                     LAGVGGAVVFVPVVIALGMTPAIAVGTGILTSTGIAAFGVLGHLLHLGSLPILVTILV
                     VVTFSAGTIIGSMKSEKWSKIKLNALVTLICLATGIGFIVNNL"
     gene            3800..>4552
                     /locus_tag="PLS90_15540"
     CDS             3800..>4552
                     /locus_tag="PLS90_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPK03860.1"
                     /translation="MSEHDLNHPPSADAASAPGGRNRRPGLLGLIYALLGTLAVSTSY
                     VTAKRGLEGFNAATFSLVWTGAAAGYALLLVLITGPARALAAPAACRSRIGWIGLATG
                     ASMLLSWAGLDRLDPTYAAMLWRFSPGITLLLGFVVHGERLSRGAGPLAALMLLGGVI
                     STLGQGRVALTGVVLTLLACLAVSAQFILAKSVLARMAPLALVFYRNALGVAPIALWG
                     IAAGQLNFDVAPRYWVVTLAGALLAPCASFILY"
BASE COUNT          712 a         1392 c         1556 g          892 t
ORIGIN      
        1 ttcgaggagg tgctgacagc cgagcatctg ctcaaatcga tcggcgccgc gccggcgggg
       61 atcggcgcca ccggcatgta tccggagggg atcgatggac gcgaagacgt gcagcggctg
      121 ctgatggagc gcggctatca ttgggtgtcc tcccggttca acctgaagcg gacgctccac
      181 gagatgcagc cgtatcgcta tcccaacggg ctgctggagc tgccgtgcgc gggatggtcg
      241 gatttcacct ggttttatgc atccgaccag ccgacgctgc cggagtatga cgtgggcggg
      301 agcccgacgc tggagcagtt catccggcat gttcaggatc tgacgacgct ggcggcccgg
      361 gacggcctga tcctggcggt ctgcctgcat ccgggcctcc tggccgcgca cgatccggag
      421 ctgcattgcg tgccgcgcgt gatccagcac gcgcgccgcc tgggcgtgga gccggcggat
      481 ctgcgcacga tcgcggccgc ctgtccgccc gctcgcggcg gctgagattt tcggctggct
      541 tcagttgcag catctcgatt caatgggctg gcgcaattcg aaataccaac acagcggagg
      601 aaatcggcat gaccctcgtc catgagggaa cgggactgct ccacggcgtt ttcccgatcc
      661 tggcgacatg cttcaaaccg gacgattcca ttgactggga gagccagcag cggctgatcg
      721 atttctgtat cgagggtggg gtgcatggcc tcgtgatcct ggcaaacgct tcggaaggcc
      781 acctgctctc cgaacaggag aagcgcgagc tgatcgtttt ctgcctgaaa cagatcgccg
      841 gccgcgttcc ggtgatcgcg acggtgaatc atccctcggc gaaagtcgca gcggaactgg
      901 cgcgccatgc cgaacagcac ggggccgcgg ccgtcatggc actccccccg ttttttggcc
      961 ggtggcggtc gggccaggat gagattttcc attttttcag cacgctcaat gcggcggtgc
     1021 ggatcccgat cgtgattcag gaccatgtcc tgtcggacat cacgctgccg gtggcgttcc
     1081 tgattgagct ggggaaaaag ctggagcacg tccgctatgt gaaactggag gcgggaaata
     1141 tcctgcacaa ggcctggaaa atcgccacca cgccacagca gccgtttctg ggcatcttcg
     1201 gcggcaacag cggcgccttc atgcccgagg aggtggaagc cggctgcacc ggcacgatgc
     1261 cggcctgcta catgccggag gtcttccgaa ccgcgtggga catggtggcg gcgggcgcgg
     1321 gcgacgccgc ggtggcttat ttcacgccct tctcgcgcct ggcgtcctat gaaaaggatg
     1381 tcgccaatcg ctgcgtgtgg aaagagatcc tggttgcgcg ggggatcatt cagcacaatc
     1441 atgtgcgcga gcccgcgccc gggtatgccg acccctggca acggcagcag ttgcttcggc
     1501 tggccggcag aatggggctg ctggctggcg cggccgccgg aggaaagagc caatgagcct
     1561 cgtgatcgag aagattgacg tctatattct gaagctcgat aagcactatc aaatccgcgg
     1621 cgacatggac gcccccgagc ttttcccggg gaccgactac tatctggagt cctcgctgcg
     1681 gcaggtttcc agcctcaaga ccgaaacctg cctgatcaag ctgacaacca accgggggct
     1741 ggaggggtgg ggcgaggcgc aggcgccgat cgtccccgag accgcggcct cgatcatcac
     1801 ccggctgctg gggccgttcg tgatcggcaa ggacccgctg cgcaacgaag tgctggtcga
     1861 cgggatgtcg catctgatgg aggtgcgcgg gcatggttcg gggttcatgc tcgacgcgat
     1921 cgccgccatc gatctggcgc tgtgggattt gaagggcaaa tactacggcg cgccgatctt
     1981 tgagttgctc ggcggaccct atcgcacgcg gctgccggcc tacgtctcgg ggttgcggca
     2041 gcccacgctg gcggagcagt gcgtcgcggc ccgggagtat cgcgaccagg ggattcaggg
     2101 aatcaaactg tttctgggcc agggcgtcga ggccgacgtc cataccatgc gaaccatccg
     2161 cgaagcggtc ggccccgcct tccggcttca gaccgacatc atgtggaaat atgggctggc
     2221 ggagtccaaa cggctggccc gcgagatgga agcggtcaac atggagtgga tggaagcgcc
     2281 gatggcgccc tatgacctgg aagggcatcg cgaactggcg cgcagcacgt cggtgccgct
     2341 ggcgatcggt gaaaccttcc gcgccgtcca cgaattcatg ccgtggttca aggcgcaggc
     2401 gctggcgatc gcgcagcccg acatcatgcg catcggcctg acggttggct gcaagatcgc
     2461 ggcgctcgcc ttggcgttcg acaagcagct cgcgccgcat gtcggggtgt gcacgagcat
     2521 cggcaccgcc gcggcgtggc atttctcggc cagcctatcg aattttctta ttcaggaatg
     2581 gcaggcgcag ttgtttgatt cgaccaatgc gctgctgcgc aaaccgctga gcgtggaagg
     2641 gggttatctc gtcgtcccgt cggatcccgg gctcgggatc gaggtggatg aagccgccgt
     2701 gcggcgccat gcggccgacc attgggaaat caccgcgagc ggaattcacc atgctgcttg
     2761 agcatggcct gctcggcgtg ggcagtttcg cgatcggcct cgtcgtcggt tcgaccagcc
     2821 tgggcggctt tctgctcgtc ccgctgatgc tgatgcaggc ggggatgacg ctcagcacgt
     2881 cggtgctgat ttcgctcctc tcgttcgtgc cctcgggcat ggtcaacacg tatcagtatg
     2941 cgtgccggaa gtgtgtcgac gtcaaactct tcgcgttgct gctggtgtcg agcctgccgg
     3001 gctcctacct tggggtttac tggagccatg cggtttcggc gacggcctcg cgcaggctgc
     3061 tgggctgctt tctactggtg accggggctt ttttgtgtct gcgcttgctc ggcgagatca
     3121 aacggcgggg cgatggcgcc gccacctcgg agcgcgtaca actggccggc aagccggggc
     3181 ggattctatt gtttgtggcg ctgggcgtga tggccggctt gtcctcgacg ctcgcgggcg
     3241 tcggcggcgc cgtggtgttc gttccggtcg tcatcgccct gggcatgacg cccgccatcg
     3301 cggtcggcac gggcatcctg acgtccaccg gaatcgcggc gttcggcgtg ctgggccacc
     3361 tgctgcatct cggctcgctc cccatcctcg tcacgattct ggttgtcgtt accttttcgg
     3421 cgggaaccat catcggttcg atgaagtccg aaaaatggtc caaaatcaag ctgaacgccc
     3481 tcgtcacgct gatctgcctg gccacgggca ttggatttat tgtgaataat ttatgagtgg
     3541 ccaatcccgg taagagtcat tggatgaaca gccagggaat ttcgcgccgc cgcccgcaac
     3601 atgggcatcg ctccggaagc gaaggatggc gcctggtcgt gactcagcct gcgtccagtt
     3661 gatcgggtcg attgatgctt taattcattc cattacgatt cccgcctgtc gaattgcagg
     3721 gtcatctgtc gccgtgtgaa aatccaatcg cgtttccggc tcgaacccgt gacgatgtcg
     3781 cgcaaaggaa cggcccggcg tgtcggagca tgatctgaac cacccccctt ccgccgacgc
     3841 ggcgtccgcg ccgggtgggc gaaacaggcg acccgggttg ctcggcctga tttatgccct
     3901 gctggggacg ctggcggttt cgacgagtta cgtgacggcg aagcgcggtc tggaggggtt
     3961 caacgcggcg acgttcagcc tggtctggac cggcgccgcc gccggttacg cgctgctgct
     4021 ggttctgatc accggccccg cgcgggcgct ggcggcgccc gccgcctgcc ggtcgcggat
     4081 cggctggatc ggactggcga cgggggcgtc gatgctgctg agctgggccg gcctggaccg
     4141 gctcgacccg acctacgccg ccatgctgtg gcgcttcagc ccgggcatca cgctgctgct
     4201 ggggtttgtg gtccacggcg agcggctttc gcggggcgcc gggccgctgg ccgcgctgat
     4261 gctcctgggc ggggtgatca gcacgctcgg ccagggccgg gtggcgctca ccggcgtcgt
     4321 gctgacgttg ctcgcctgcc tggcggtctc ggcgcagttc atcctggcga agtcggtgct
     4381 ggcgcggatg gcgcccctcg cgctggtatt ttatcgcaac gcgttggggg ttgcgccgat
     4441 cgcgctctgg ggcatcgcgg ccgggcagtt gaacttcgac gtggcgcccc gctactgggt
     4501 cgtcacgctg gcgggcgcgc tgctggcccc ctgcgccagt ttcatcctgt at
//
LOCUS       DAOPCO010000318         4541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15805_length_4541_cov_3.465448, whole genome shotgun sequence.
ACCESSION   DAOPCO010000318 DAOPCO010000000
VERSION     DAOPCO010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15805_length_4541_cov_3.465448"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLS90_15545"
     CDS             complement(<1..521)
                     /locus_tag="PLS90_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03861.1"
                     /translation="MKTHHWVGDSEIESALLVPQEDVDRRAPDVRIMVLANLAKSGSK
                     SHFPHHELMECHRGIRLIVDRHNLFEVILSGRQTVFDINSNGPEILLKRGAVVIRGGR
                     FYFRGLPAPGCGLHSGRWGGNVIGLVGQTVDNTCQTPRSRIDLDLQSFIAQGERFAPQ
                     WSMKTGYHHGQGKA"
     gene            579..2243
                     /locus_tag="PLS90_15550"
     CDS             579..2243
                     /locus_tag="PLS90_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03862.1"
                     /translation="MLGPRESYRSEPLFLGAYRNEGIYLFRELRKQLEVIDQPISHGS
                     HMNWEQQILDWGEVWAMRKYLRDIMPPHDTRRPGYYVRAVAMVGGSTSNTWGEEAEFH
                     VHFEEKHLEGSRAFVDQVAELGHVPHIEWATEWYGVGGYTNPTPDHMLERAGPGQKPP
                     VNPYWQQVIEYAQSKGIAGGIFETVTRNFAEGRDDWKVLQVDGTPWTWDPDDRPINCW
                     ANRDYVDWRLEVTDQAVNDFDLYMVAWDSASPAFWTWFGFPEVQTECHATNHGHPPGD
                     ITYHLFRNIAWFLEELQARHPRLALRVACGATRGYPWILKHLIEYHPNLYDGEMGSTY
                     WVSYNFRFLPIYKSGLLLSANTREEFEYLLLRSIANSDHFMLWPDAVPIALEHKAFWD
                     RWITWADEHIDFLRTGRTLFREPWGDDMVASLHPALEGSLPADDAQISGVSHCLGDRG
                     FLFLFNPDDQPRAAQIPVNHWLGLESGDRFAITTLYPGDGESLGVFNRGDLLTLRVEP
                     DRACVLAIEPAADADAAPIPQDVDGLPVDKAFLTWDEIPWREIMIP"
     gene            2314..4026
                     /locus_tag="PLS90_15555"
     CDS             2314..4026
                     /locus_tag="PLS90_15555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPK03863.1"
                     /translation="MEHVTFQTIDYIVFFSYMAGILAFGLFLAFYRKQETARDYFLAG
                     DRLPWYAIGASVLASNISTEHFIGMMGWAYVYGFVIANFEWGAWFTWSIALWFFLPFY
                     LRTRIFTVPEFVELRFSRSVRTLLSLASLTTYLTALMAGVLFAGAKVLNAMLGIPLHW
                     GVLALGVATGVYCIFGGLLAVVWTDFVQCILFIVGGGLVTWLGLKRVGGLGNLMTEMP
                     EQFFMFHVQHEQAPIIAFYLCSFFVGAYYMSSNQFMMQRCMGGRSHWDGAMGLVFSNF
                     LKLLMPLIVVLPGIVAARMFPNLPDPDQSYPILVAELLGPGLLGLLMSGLAAAMMSTV
                     SSALNSASTLFTLDIYAPLAKLNENDPRLVRMGKWVTTGLLVLGMALGMFYSRLTDPV
                     TGAPMPVFGIIMNIFFFIGPPLSIVFLAGIFWPRATPKAAVWTIIMGYVGGLVSELVI
                     FKPYDRMFAPLRAFFFPTDSLVNFKLWFDGTAFAATFRNFLYFAIWIGMISLIVMVVV
                     SLLTKPRKREEIAHLLWRPSVMKVDSKPGERTTRSLVFWWAVVMALTAILYGYFAWFQ
                     LSHS"
     gene            4040..>4541
                     /locus_tag="PLS90_15560"
     CDS             4040..>4541
                     /locus_tag="PLS90_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03864.1"
                     /translation="MSRSQRHRSIPALLCLALVALLPQLPARAAVPAEGFRIENQIPA
                     GAQRVGMSWWATSDGISQTAATREPQKLLFGEKDWNDYTIRAQVQLLNPDVGSRAGLM
                     LQAAGTGTYVAFWLIKRQNGPYAELRFEQSEGLDLTGDQSWLAELDLTQWHELRADVH
                     GAEIACY"
BASE COUNT          769 a         1632 c         1219 g          921 t
ORIGIN      
        1 gctttgccct ggccatgatg ataaccggtc ttcatggacc actgcggggc gaaccgctcg
       61 ccttgcgcaa tgaaggattg gagatcgaga tcgattcggc ttcggggggt ctgacacgtg
      121 ttgtcaaccg tctgaccaac gagacctatg acatttcctc cccagcggcc ggagtggaga
      181 ccacagccgg gcgctggcag accacggaag tagaatcgac ctccacgaat gacgacagcg
      241 ccgcgtttca gcaggatttc gggcccgttg ctgttgatat cgaataccgt ctgccggccg
      301 gacaggatta cctcgaaaag attgtgacgg tccacaatca atctgattcc acgatgacac
      361 tccatcaact cgtgatgggg gaaatgactt ttactcccgc ttttcgccag attcgcgagc
      421 accatgatcc gaacatctgg cgcacgccga tcaacgtctt cctgcgggac aagcagggcg
      481 gattctatct cggaatcgcc aacccaatga tgcgttttca tactcggggc gccacgcccc
      541 gcagcaatgt gatggccctg acttatgagc ccgcgtgggt gttgggtccg cgagagagct
      601 atcggtccga gccgctgttc cttggggcct atcgcaatga agggatctac ctcttccgcg
      661 aattacgcaa gcagctcgaa gtcatcgatc aacccatctc gcatggttcc catatgaatt
      721 gggaacagca gattctggac tggggcgagg tctgggcgat gcgcaaatat ctgcgcgaca
      781 tcatgccacc gcacgacaca cgccgtcccg gctactacgt tcgcgccgtg gccatggtcg
      841 gcggcagcac cagtaacacc tggggtgaag aggcggagtt ccacgttcac ttcgaagaga
      901 agcatttgga aggttcgcgg gcattcgttg accaggtcgc cgaactgggc cacgttccgc
      961 acatcgagtg ggcgacggag tggtatggcg tcggcggata cacaaacccc acgccggatc
     1021 acatgctgga gcgggcggga ccaggccaga aaccgccagt caatccgtat tggcagcagg
     1081 tgatcgagta tgcccagtcg aagggaattg ctggtggcat ttttgagacc gtcacgcgga
     1141 acttcgccga agggcgcgac gactggaaag tgttgcaggt tgacggtacg ccctggacgt
     1201 gggaccccga cgaccggccg atcaactgct gggccaaccg cgactatgtc gactggcgcc
     1261 tcgaagtgac cgaccaggcc gtcaacgatt tcgacctcta catggtcgcc tgggattccg
     1321 cctcccccgc cttctggacc tggttcggct tccccgaagt ccagaccgag tgccacgcaa
     1381 ccaaccacgg ccatcccccc ggcgacatca cctaccatct gttccgcaac atcgcgtggt
     1441 tcctggagga gttgcaggcg cgccatcctc gcctcgcgct gcgcgtcgcc tgcggcgcca
     1501 cccgcggcta tccctggatt ctgaaacacc tgatcgaata ccaccccaat ctctacgacg
     1561 gcgagatggg ctccacttac tgggtcagct acaacttccg cttcctgccg atttacaaat
     1621 ccggcctgct gctctcggcc aatacccgcg aggagttcga ataccttctc ctccgatcca
     1681 tcgccaactc cgatcacttc atgctctggc ccgatgccgt ccccatcgcc ctcgaacaca
     1741 aggccttctg ggaccgctgg atcacctggg ccgatgagca catcgacttc ctccgcaccg
     1801 gccgcaccct cttccgcgaa ccctggggcg acgacatggt cgcttcgctc catccggccc
     1861 tcgaaggcag tctgccggcc gacgacgccc agatcagcgg cgtcagtcac tgccttggcg
     1921 accgcggctt cctcttcctg ttcaatcccg acgatcagcc ccgcgccgcc cagatccccg
     1981 tcaatcactg gctcggcctg gaatcgggcg accgcttcgc gattacgacc ctctaccccg
     2041 gcgacggcga gagcctcggc gtattcaacc gcggcgacct cctgaccctg cgcgtcgagc
     2101 ccgatcgcgc ctgcgtcctg gccattgagc ccgccgcgga cgcggacgcg gcgccgatcc
     2161 cccaggacgt cgacggcctc cccgtcgaca aggccttcct gacctgggac gagatcccgt
     2221 ggcgcgaaat catgattccg tgagatgctt ttttcctgat ccagcaatcg cttcctcgat
     2281 tccaaccgcc ccgacggtag aaatgagcgc acgatggagc atgtaacctt tcagacgatc
     2341 gattacatcg tctttttctc ctatatggcc ggcatcctcg ccttcggcct gttcctcgcc
     2401 ttctaccgca agcaggagac ggcgcgcgat tacttcctcg ccggcgaccg cctcccgtgg
     2461 tatgccatcg gcgcctccgt cctcgcctcc aacatcagca ccgagcactt catcggcatg
     2521 atgggctggg cctatgtcta tggcttcgtc atcgccaatt ttgaatgggg cgcctggttc
     2581 acctggtcca ttgccctctg gttcttcctc cccttctatc tgcgcacgcg catcttcacc
     2641 gtccccgaat tcgtcgagct gcgcttcagc cgctcggtcc ggacgctgct ctcgctcgcc
     2701 tcgctgacga cctacctcac cgcgctcatg gccggcgtcc tctttgccgg cgccaaggtc
     2761 ctcaatgcca tgctcggcat ccccttgcat tggggcgtcc tcgcgctcgg tgtcgccacc
     2821 ggcgtctact gcatcttcgg cgggctgctc gccgtcgtct ggaccgattt cgtccagtgc
     2881 atcctcttca tcgtcggcgg cggcctggtc acctggctcg gcctcaaacg cgtcggcggc
     2941 ctgggcaatc tgatgaccga aatgcccgag cagttcttca tgttccacgt ccagcacgaa
     3001 caggccccga tcatcgcctt ctacctttgc agcttctttg tcggcgccta ttacatgagc
     3061 agcaaccagt tcatgatgca gcgctgcatg ggcggccgtt cgcactggga cggcgccatg
     3121 ggcctcgtct tctccaactt cctcaaactg ctcatgccgc tgatcgtcgt cctgcccggc
     3181 atcgtcgccg cccgcatgtt ccccaacctg cccgaccccg atcagtccta cccgatcctg
     3241 gtcgccgaac tgctcggccc cggcctgctc ggcctgctca tgtcgggcct ggccgccgcg
     3301 atgatgtcca ccgtcagctc ggccctcaat tccgcctcca cgctcttcac cctcgacatc
     3361 tacgcccccc tcgccaagct caatgaaaac gatccgcggc tggtccgcat gggcaagtgg
     3421 gtcaccaccg gcctcctcgt cctgggcatg gccctcggca tgttctactc gcgcctgacc
     3481 gaccccgtca ccggcgcgcc gatgcccgtc ttcggcatca tcatgaatat tttcttcttc
     3541 atcgggccgc ccctttcgat cgtcttcctc gccggcattt tctggccccg cgccaccccc
     3601 aaggccgccg tctggacgat catcatgggc tacgtcggcg ggctcgtctc cgaattggtc
     3661 atcttcaagc cctacgaccg gatgttcgcc ccgctgcgcg cgttcttctt ccccaccgat
     3721 tccctggtga atttcaagct ctggttcgat ggcaccgcct tcgccgcgac cttccgcaac
     3781 ttcctctact tcgccatctg gatcggcatg atttccctga tcgtcatggt cgtcgtcagc
     3841 ctgctgacca aaccccgcaa gcgcgaggag atcgcccacc tgctctggcg cccctccgtg
     3901 atgaaagtcg actccaagcc cggcgaacgc accacccgca gcctcgtctt ctggtgggcg
     3961 gtcgtcatgg ccttgaccgc catcctctac ggctacttcg cctggttcca gctctcgcat
     4021 tcctgaaagg atctgtttta tgtcgcgctc tcaacgtcac cgctcgatcc ccgcgctgct
     4081 ctgcctggcg ctcgtcgcgc tccttcccca actgccggcc cgggccgccg tccccgccga
     4141 gggcttccgc atcgaaaatc aaatccccgc cggcgcccag cgcgtcggca tgtcgtggtg
     4201 ggcgacctcc gacggcatct cgcagaccgc cgccacccgc gaaccgcaga agctcctctt
     4261 cggcgagaag gactggaacg attacacgat ccgcgcccag gtccagttgc tcaatcccga
     4321 cgtcggctcg cgcgccggcc tgatgctcca ggccgccggc accggcacct acgtcgcctt
     4381 ctggctcatc aagcgccaga acgggcccta cgccgaactc cgcttcgaac agagcgaggg
     4441 cctcgacctg accggcgatc agagctggct cgccgagctc gacctgaccc agtggcacga
     4501 actgcgcgcc gacgtccacg gcgccgaaat cgcctgctat g
//
LOCUS       DAOPCO010000319         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_15826_length_4536_cov_4.488953, whole genome shotgun sequence.
ACCESSION   DAOPCO010000319 DAOPCO010000000
VERSION     DAOPCO010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15826_length_4536_cov_4.488953"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /gene="argS"
                     /locus_tag="PLS90_15565"
     CDS             <1..1732
                     /gene="argS"
                     /locus_tag="PLS90_15565"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPK03865.1"
                     /translation="TPFFSIETAVEQLLRDALRAFIGQLRTEADTAGDEQRLTAALDL
                     DPDTVCLEQPRNPRHGDWATGIAMQLARPLRRNPFEIAQAIVAFFPPSDLIAPPEIAR
                     PGFINLRLADGALARLVRHVLDAGAAWGRSDAFAGRKVLVEFVSANPTGPLHIGHCRG
                     AVVGDALARLLAAVGYDVSREYYYNDAGVQMKKLGESLRARYLQALGEDAPMPEDGYM
                     GDYMVDLGRRLMEERGDALRATADTRPFTLYAAEHILKLIDDDLKALRIDFDTWFSET
                     TLHEDGSVRRVLERLKSERRIYEKDGAWWLRTAALGDEKDRVVIKSDGEYTYLAPDIA
                     YHEYKFNRGFDRLVNVLGADHHGYVPRLKASVAALGYRPEQLDCVLVQMVGIKRGGET
                     IKLSTRAGDFITLKQVIDEIGPDVTRFLFLMRSPDSQMVFDFDLARDTSMDNPLYYVQ
                     YAHARCCSLMRKAEECGAPWRGGGDARLDRLTAAEEKAIVRQIDRLPASVQDAASRCD
                     PMLVTSYLTDLATAFHAYFTAGNRDPALRVVQTEDTELTQARLTLIEALRLTLANGLR
                     LLGVEPLERL"
     gene            1998..2756
                     /locus_tag="PLS90_15570"
     CDS             1998..2756
                     /locus_tag="PLS90_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03866.1"
                     /translation="MIRQIFFRFFWNTYDHLGPWVLISLAAALASLPLLTAPAAWGGV
                     LACAARADQENGISPRDWLAGTLRHGMASTILGLAIGLGLVLCALNIFFYMSPRFLRE
                     LPSIARVALGGVFLWILVFGFIALNVAWAFLVLQDLPLRKALKRGLIVLIAHPISLLF
                     TFAIAAFLVFAMGLTVVGLLFLPALLGNLVMGIAAGAVEYYEVKEDQALRARIEREGH
                     RTWSELRELEEREAARFRRHDRGWRDIFKPWDLQ"
     gene            2991..3689
                     /locus_tag="PLS90_15575"
     CDS             2991..3689
                     /locus_tag="PLS90_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HPK03867.1"
                     /translation="MKRSEINHLIREAIEFFNRHQFHLPAFAHWTPADWQHKGPEARE
                     IARHLLGWDITDFGLGDFYKTGLLLFTIRNGQPQNLRNGTGKVYAEKIMIVREDQLTP
                     WHHHRQKTEDIINRGGGRLVIELANATPDGKGLADSEVIVSIDGVEHRLAAHDQVTLN
                     PGESITLTTGLQHKFWGQPGAGWVLTGEVSCVNDDTQDNFFYEQVGRFPEIEEDEPPF
                     RLLVGDYPRYYAHA"
     gene            3711..4469
                     /locus_tag="PLS90_15580"
     CDS             3711..4469
                     /locus_tag="PLS90_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03868.1"
                     /translation="MQLNGHVVWITGAARRLGRAMALDLARRGADLVVHYHHSRAEAE
                     QVAGEIERLGRRVLTVQADLRSVAAIRHCIDRIEHHFGRLDVLINNASNFERVEFDRI
                     TEEDWDLSLDTNLKGPFFCAREAARLLRRDGGGKIINLADWSALRPYRHYAPYMIAKG
                     GIVTMTQVLALELAPEVQVNAVAPGVVLPQPDVAAEAMDKLLRQVPLGRAGSPDDIVA
                     TVAFLLEGSDYITGQVICVDGGRLIAQPPRPEHA"
BASE COUNT          734 a         1808 c         1240 g          754 t
ORIGIN      
        1 gaccccgttt ttttcgattg aaaccgctgt ggaacaactc ctgcgcgatg cgttgcgcgc
       61 cttcatcggc cagttgcgca ccgaggccga caccgccggc gacgagcagc gcctcaccgc
      121 ggcgctcgac ctcgaccccg acaccgtctg tctcgagcag ccccgcaacc cgcgccacgg
      181 cgactgggcg accggcatcg ccatgcaact cgcccgcccg ctccgccgca atccgttcga
      241 aatcgcccag gcgatcgtcg ccttctttcc gccatctgat ctgatcgcgc cccccgagat
      301 cgcccgcccc ggcttcatca acctgcgcct ggccgacggc gccctcgccc gcctcgtccg
      361 ccacgtcctc gacgccggcg ccgcctgggg ccgatccgac gccttcgccg gccgcaaagt
      421 cctcgtcgag ttcgtcagcg ccaaccccac cggcccgctg cacatcggcc attgccgcgg
      481 cgccgtcgtc ggcgacgccc tcgcccgcct cctcgccgcc gtcggctacg acgtctcgcg
      541 cgagtactac tacaacgacg ccggcgtgca gatgaaaaaa ctcggcgaat cgctccgcgc
      601 ccgctacctg caggccctcg gcgaggacgc cccgatgccc gaggacggct acatgggcga
      661 ctacatggtc gacctcggcc gccgcctcat ggaggaacgc ggcgacgccc tccgcgccac
      721 cgccgacacc cgccccttca cgctctacgc cgccgagcac atcctcaagc tcatcgacga
      781 cgacctcaag gccctgcgca tcgacttcga cacctggttc agcgagacga ccctgcacga
      841 agacggcagc gtccgccgcg tcctcgaacg cctcaagtcc gagcgccgga tctacgaaaa
      901 agacggcgcc tggtggctgc gcaccgccgc cctcggcgac gagaaggacc gcgtcgtcat
      961 caaatccgac ggcgaataca cctacctcgc ccccgacatc gcctatcacg aatacaagtt
     1021 caaccggggc ttcgaccggc tggtcaacgt cctcggcgcc gatcatcacg gctacgtccc
     1081 gcgcctcaag gcttcggtcg ccgccttggg ctaccgcccc gagcagctcg actgcgttct
     1141 cgtccagatg gtcggcatca agcgcggcgg cgaaacgatc aagctctcga cccgcgccgg
     1201 cgacttcatc accctcaagc aggtgatcga cgagatcggc cccgacgtca cccgcttcct
     1261 ctttctcatg cgctcgcccg acagccagat ggtcttcgat ttcgacctgg cccgcgacac
     1321 ctcgatggac aacccgctct attacgtcca atacgcccac gcgcgctgct gcagcctgat
     1381 gcgcaaggcc gaggaatgcg gcgccccctg gcgcggcggc ggcgacgccc gcctcgaccg
     1441 cctcaccgcc gccgaggaga aggccatcgt ccgccagatc gaccgcctcc cggcgagtgt
     1501 ccaggacgcg gcctcccgct gcgacccgat gctcgtcacc agctatctga ccgacctggc
     1561 gaccgcgttc cacgcctatt tcaccgccgg caatcgcgat ccggcgctgc gcgtcgtcca
     1621 gaccgaggac accgaactga cccaggcccg gctgacgctg atcgaagccc tgcgcctgac
     1681 cctggccaac ggcctgcgcc tcctcggcgt cgaacccctg gagcgtctct agccggcatg
     1741 gcaagactca cgatggaaca gcaacacttc tcagcaacgg tatcggtatc ggtctcgcaa
     1801 tcgctattgg catcggggtc ggtactataa ttgagcccgc gtcgcgcagg gatgccggaa
     1861 ttctctgcgg cccgctttca cgacgcctcc ggcgtccctt gaaaaactga aagcagcctt
     1921 catctttctc gtatgcccct catgtccctc acgtccctta cgtcctttac atccctcccg
     1981 atctgggccg acctgacatg atccgccaga tcttcttccg cttcttctgg aatacctacg
     2041 accacctcgg gccgtgggtc ctgatcagcc tcgccgccgc gctggccagc ctgccgctgt
     2101 tgaccgcccc ggcggcgtgg ggcggcgtcc tcgcttgcgc cgcgcgcgcc gatcaggaaa
     2161 atgggatcag cccgcgcgac tggctcgccg gcacgctccg ccacggcatg gcctccacga
     2221 tcctcggcct cgcgatcggc ctcggcctcg tcctctgcgc gcttaacatt ttcttctaca
     2281 tgtcgccccg cttcctgcgc gagctgccct cgatcgcccg cgtcgcgctc ggcggcgtct
     2341 tcctgtggat tctcgtcttc ggtttcatcg cgctgaacgt cgcctgggcg ttcctggtcc
     2401 tgcaggatct gccgttgcgc aaggccctca agcgcggtct catcgtcctc atcgcccatc
     2461 ccatcagcct gctcttcacc ttcgccatcg ccgcgttcct ggtgttcgcc atggggctca
     2521 ccgtcgtcgg cctgctgttc ctgcccgccc tgctggggaa cctggtgatg ggcatcgccg
     2581 ccggcgccgt cgagtattac gaagtgaagg aggaccaggc cctgcgcgcg cgcatcgagc
     2641 gcgaggggca ccgcacctgg tccgaactgc gcgagctcga ggaacgcgag gcggctcgct
     2701 tccgccgcca cgaccgcggc tggcgcgata tcttcaaacc ctgggatctg cagtaacccg
     2761 ccccaccccg agagttcgtc cctcgtccct cgtctcggct ctcgaaaccc cgtccttcac
     2821 cccttcgccg tgccgtcgca tcaagcccaa ctcctttcgc tctcgtctcc cccctttccc
     2881 ccgtctcatt ccgcctgccg gttgaaaatg atccgccgcc cggggtagct tggtccatgg
     2941 cgcgcgcggc gcgccgccgc cggatgctcc cacgaaatga ggcgcgtgcc atgaaacgct
     3001 cggaaatcaa ccacctgatc cgcgaagcca tcgaattctt caatcgccat cagttccacc
     3061 tgcccgcctt cgcgcactgg acccccgcgg actggcaaca caagggcccc gaagcccgcg
     3121 agatcgcccg ccacttgctc ggttgggaca tcaccgactt cggcctgggt gatttctaca
     3181 agaccggcct gctcctcttc accatccgca acggccagcc gcaaaacctg cgcaatggaa
     3241 ccggcaaggt ctacgccgaa aaaatcatga ttgtccgcga agaccagctc accccgtggc
     3301 accatcaccg ccagaagacc gaggacatca tcaatcgcgg cggcggccgc ctcgtcatcg
     3361 aactggccaa cgccaccccc gacggcaagg gcctggccga tagcgaggtc atcgtctcca
     3421 tcgacggtgt cgaacatcgc ctcgccgccc acgaccaggt caccctcaac cccggcgaga
     3481 gcatcaccct caccaccggc ctccagcaca agttctgggg ccagccgggc gccggctggg
     3541 tcctgaccgg cgaagtctcc tgcgtcaacg acgacaccca ggacaatttc ttctacgagc
     3601 aagtcggccg gtttcccgaa atcgaggaag acgaaccccc gttccgcctc ctcgtcggcg
     3661 actacccccg atactacgcc cacgcctgac ggcgcgcgcc tggaccccgc atgcaactca
     3721 acggacacgt cgtctggatc accggcgcgg cccggcgtct cgggcgggct atggcgctgg
     3781 acctggcccg ccgcggcgcc gatctcgtcg tccattacca ccattctcgc gccgaagccg
     3841 agcaggtcgc cggcgaaatc gaacgcctcg gccgccgcgt gctcaccgtt caggccgacc
     3901 tgcgctccgt cgccgcgatc cgccactgca tcgaccggat cgagcaccac ttcggccgac
     3961 tcgacgtcct catcaacaac gcctccaatt tcgagcgcgt cgagttcgac cgcatcaccg
     4021 aggaggactg ggatctcagc ctcgacacca acctgaaggg cccgttcttc tgcgcgcgcg
     4081 aggccgcccg cctcctccgg cgcgacggcg gcggcaagat catcaacctc gccgactggt
     4141 cggccctgcg gccctaccgc cattacgccc cctacatgat cgccaagggg ggcatcgtca
     4201 caatgacgca ggtcctcgcc ctcgaactcg cccccgaggt tcaggtcaac gccgttgccc
     4261 ccggcgtcgt gctgccccag cccgatgtcg cggccgaggc catggacaag ctgctccgcc
     4321 aggttcccct gggccgcgcc ggttcgcccg acgacattgt cgccaccgtc gccttcctcc
     4381 tcgaagggtc cgactacatc accggccagg tgatttgtgt cgacggcggc cggctcatcg
     4441 cccaaccccc ccgccccgaa cacgcctgac cccatgtgag cgccattttg ggaccgctac
     4501 tcttccgagg ggcctcgccc ccccaaagcc tacccc
//
LOCUS       DAOPCO010000320         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16006_length_4488_cov_4.940898, whole genome shotgun sequence.
ACCESSION   DAOPCO010000320 DAOPCO010000000
VERSION     DAOPCO010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16006_length_4488_cov_4.940898"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLS90_15585"
     CDS             <1..1117
                     /locus_tag="PLS90_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03869.1"
                     /translation="AFGWEVAREDHFAARLAPDLARALGVDRVEPLIVAVPGHTPAQQ
                     VELFVRQTLAENPAFVLFQFDLNATRWAWCVRRPAAACRVLLLARPIDLIRGRLALTA
                     LAGSPPTDPPDAAVDLTGWEGAGNALQYLAEACRERRIPVFLFATAPPAWGGSSALED
                     GRLDRLRDLCRNAGIHYLPVLEWVEGVVASGAMEPASVMVDRAPQAHPNRLGHLLLLS
                     ALAPELTDGLAQAGLTAAESSPAPELRRQYQREFVQAASEGLYAVEDWGRPVQWTRER
                     VSRLYFPEGRRLIQRYHIGTRDISPTNPVIVQMHVPGTEEVRRIYWRGNQEDALAFDL
                     GEPWPPAVRVEIRVNRTFREGKWGRLLGIALFEPVYE"
     gene            complement(1128..4178)
                     /locus_tag="PLS90_15590"
     CDS             complement(1128..4178)
                     /locus_tag="PLS90_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPK03870.1"
                     /translation="MTPAAPTATAPAAGRLHRLELGLKPGVADARGDRTLHAIRRHLG
                     LDVAAVRTRDLYTFDADLRGEDLARIAAEFTDPVIQLGGSPRLEADDPPGWDWMIIIG
                     FKPGVTDNVGRTSRTAVAALLGRPLAEDEGVYTSTAWVLAAPALTRADAQRIADELLA
                     NPLIQSTQIISRAEWEAGAPRPGVPRVQSATRPRVERIDLSGSDDDLLRISQSRTLAL
                     SLAEMRAIRDYFRQAGSDPRRRELGLNDQPTDVELEMLAQTWSEHCKHKIFAARIDYT
                     DETGKTQTIDSLFKTYIRGATQKIGERIDWLVSVFHDNAGVIAFNDRIDLVYKVETHN
                     SPSALEPYGGAMTGIVGVNRDTLGTGMGAEPLVNVWGYCFASPFTPPAAVPDGVMHPR
                     RLRDGVHQGVIDGGNQSGIPYGLGWEFFDARYLAKPMVYCGTLGWLPKRIRDGRPAAE
                     KVILPGDLAVMVGGRIGKDGIHGATFSSEELHKDSPVQAVQIGDPITQKKMTDFLIEA
                     RDRGLFNFIHDMGAGGLSSSFGEMAQRVGGLEVDLARAPLKYAGLQPWEIWLSEAQER
                     QSLSVPPDKIDELLALARERDVEATVLGRFNTSGYIHLKYADQHVGWIALEFLESGYP
                     RLELKAEWQPPSFPSVQPPRGDLTDVLRRLLMRCNLASRESKCRQYDHEVKGRTVVKP
                     LVGRRRDIPSDATIFLAEYDGREGIVLSHGFNAHYSDLDTAAMMASVIDEAVRRIVAV
                     GGRLDRIAGLDNFCWPDPVESPSTPDGRYKLAQLVRANQALHDVCVAYGVPLISGKDS
                     MKNDSTRGGRKISIPPSVLFSALGKIDDIGRAVTCDAKAAGDYVYLLGVTRAELGASE
                     LAWMLHDESGRDDPPAIGGRPPALAPAAALALYRAVERATRAGRVRSLHTPTLGGLAA
                     GLARVAFGGELGLRVDLRQAPAEAELTDVDLLFSETNSRFIATVAPDHAAAFEQALAG
                     QPCARVGVVTDAPRLHVTGVAGAVVIDADVLDLKRDWRRVLAET"
BASE COUNT          618 a         1390 c         1787 g          693 t
ORIGIN      
        1 ggcgttcggc tgggaggtgg cgcgcgagga tcattttgcc gcgcggctcg cgcccgacct
       61 ggcccgcgcg ctcggggtgg atcgcgttga gccgctgatc gtcgccgtgc cgggccacac
      121 gccggcccag caggtggagc tgttcgtgag gcagacgctc gccgagaacc ccgcgttcgt
      181 gctgttccag ttcgatctga acgcgacgcg gtgggcctgg tgcgtgcgcc gtccggcggc
      241 cgcctgccgg gtgctgctgc tcgcccggcc gattgatctg atccgcgggc ggctggcgct
      301 gaccgcgctg gccgggtcgc cgccgaccga cccgcccgac gcggcggtcg atctgaccgg
      361 ctgggagggt gcagggaacg cgctgcaata cctggccgag gcgtgccggg agcgccggat
      421 cccggtgttt ctgtttgcaa ccgccccgcc ggcatggggc gggtcctccg cgcttgaaga
      481 cgggcgtctg gaccggctcc gcgatctgtg caggaacgcc gggatccact atctgcccgt
      541 gctggaatgg gtcgaggggg tggtcgcctc gggggcgatg gagccggcaa gcgtaatggt
      601 cgatcgcgcg ccgcaagcgc atcccaaccg gctcggccat ctgctgctgc tcagcgcgct
      661 ggcgccggag ctgaccgacg ggctggcgca ggcggggctg acggcggcgg agagctcccc
      721 ggcgcccgag ctgcgccgcc aataccagcg ggaattcgtg caggcggcca gcgagggact
      781 ttatgcggtg gaggactggg ggcgtccggt gcagtggacg cgggagcggg tctcgcggct
      841 ttattttccg gaggggcgcc gtctgatcca gcgctaccac atcgggacgc gcgatatctc
      901 gccgaccaac ccggtcatcg tgcagatgca tgtgccggga acggaggagg tgcggcggat
      961 ctactggcgc gggaaccagg aagacgccct ggcgttcgac ctgggggagc cgtggccgcc
     1021 ggccgtgcgc gtggaaatcc gggtcaaccg aacattccgc gaggggaagt ggggccggct
     1081 gctggggatc gcgctgttcg agccggtgta tgaatagcgg gtgagcctca ggtttcggcg
     1141 aggacgcgcc gccagtcgcg tttcaggtcg aggacgtcgg cgtcgatcac gacggcgccg
     1201 gcgacgccgg tcacgtgcag ccggggggcg tcggtgacga cgccgacgcg ggcgcacggc
     1261 tggccggcga gggcctgttc gaacgccgcg gcgtggtccg gcgccacggt ggcgatgaag
     1321 cggctgttgg tttcgctgaa gagcaggtcg acgtcggtca gttcggcttc ggccggcgcc
     1381 tggcgcaggt cgacgcgcag ccccagctcg ccgccgaagg cgacgcgcgc caggccggcc
     1441 gcgagcccgc cgagcgtggg ggtgtgcagg ctgcgcaccc ggcccgcgcg cgtggcccgt
     1501 tccaccgcgc gatagagggc gagcgcggcc gccggcgcga gggcaggggg gcggccgccg
     1561 atggccgggg gatcatcgcg gcccgactcg tcgtgcagca tccaggccag ttccgaggcg
     1621 ccgagttccg cccgcgtcac gccgagcaga tagacataat ccccggcggc cttggcgtcg
     1681 caggtgacgg cgcggccgat gtcgtcgatc tttcccaggg cggagaagag gaccgagggg
     1741 gggatcgaga tcttccggcc gccgcgggtc gagtcgttct tcatgctgtc cttgcccgag
     1801 atgagcggga cgccgtaggc gacgcagaca tcgtgcagcg cctggttggc gcggacgagc
     1861 tgggccagct tgtagcggcc gtcgggcgtg gagggcgatt cgacgggatc gggccagcag
     1921 aagttgtcca ggccggcgat gcggtcgagc cggccgccga cggcgacgat gcggcggacc
     1981 gcttcgtcga tgaccgaggc catcatcgcg gcggtatcga ggtcggagta atgcgcgttg
     2041 aagccgtggg agaggacgat cccctcgcgc ccgtcatact cggcgagaaa gatcgtggca
     2101 tcggaaggga tgtcgcggcg gcgaccgacg agcggcttga cgaccgtgcg gcccttcacc
     2161 tcgtggtcgt actggcggca cttgctctcg cgcgaggcga ggttgcagcg catgaggagc
     2221 cggcgcagga cgtcggtcag gtcgccccgg gggggctgca cggaggggaa tgacggcggc
     2281 tgccattcgg ctttgagttc gagacgcggg tagcccgact cgaggaactc gagggcgatc
     2341 cagccgacgt gctggtcggc gtatttcaga tggatatagc cgctcgtgtt gaagcggccg
     2401 aggacggtcg cttcgacgtc gcgctcgcgc gccagggcca gcaactcgtc gatcttgtcg
     2461 ggcggcacgg agagcgactg gcgttcctgg gcttcggaga gccagatttc ccagggctgc
     2521 aggccggcgt acttgagcgg ggcgcgggcg agatcgactt cgaggccgcc gacgcgctga
     2581 gccatttcgc cgaaggagga gctgaggccg ccggcgccca tgtcgtgaat gaagttgaac
     2641 aggccgcggt cgcgcgcctc gatcaggaaa tcggtcattt tcttctgcgt gatcggatcg
     2701 ccgatctgga cggcctggac gggggagtcc ttgtggagtt cctcggagct gaaggtggcg
     2761 ccatggatgc cgtccttgcc gatgcggccg ccgaccatca cggcgaggtc gcccggcagg
     2821 atgactttct cggcggcggg gcggccgtcg cggatgcgct tggggagcca gccgagcgtg
     2881 ccgcagtaga ccatcggctt ggcgaggtag cgggcgtcga agaactccca gccgaggccg
     2941 taggggatgc cgctctggtt gccgccgtcg atgacgccct ggtggacgcc gtcgcgcagc
     3001 cggcgggggt gcatcacgcc gtcggggacc gccgcgggcg gggtgaaggg cgaggcgaag
     3061 cagtagcccc agacgttgac cagcggttcg gcgcccatgc cggtgccgag ggtgtcgcgg
     3121 ttgacgccga cgatcccggt catcgcgccg ccgtagggct cgagcgcgct gggggagttg
     3181 tgcgtctcga ccttgtagac gaggtcgata cggtcgttga aggcgatcac gccggcgttg
     3241 tcgtggaaga cggagacgag ccagtcgatg cgctcgccga tcttctgggt cgcgccgcgg
     3301 atataggttt tgaacagcga gtcgatcgtc tgggttttgc cggtctcgtc ggtgtagtcg
     3361 atccgggcgg cgaagatctt gtgcttgcag tgctcgctcc aggtctgcgc gagcatttcg
     3421 agttcgacgt cggtgggctg gtcgttgagg cccagttcgc ggcggcgcgg gtcgctgccg
     3481 gcctggcgga agtagtcgcg gatcgcgcgc atttcggcaa ggctgagggc gagcgtccgc
     3541 gactggctga tgcgcagcag gtcgtcgtcg gagcccgaga ggtcgatgcg ttcgacgcgc
     3601 gggcgggtcg cgctctgcac gcgggggacg ccgggtcgcg gcgcgccggc ctcccactcc
     3661 gcgcggctga tgatctgcgt cgactggatc agcgggttgg cgagcagttc gtcggcgatg
     3721 cgctgggcat cggcgcgcgt cagcgcgggg gcggccagga cccaggccgt cgaggtgtag
     3781 acgccttcgt cctcggcgag gggccgcccc agcagcgccg ccacggcggt ccgcgacgtg
     3841 cggccgacgt tatccgtcac gcccggctta aagccaatga tgatcatcca gtcccagccg
     3901 gggggatcgt cggcctcgag gcgcggcgag ccgccgagct gaatcacggg gtcggtgaac
     3961 tcggcggcga tgcgcgcgag gtcctcgccg cgcaggtcgg cgtcgaaggt gtagagatcg
     4021 cgcgtgcgga ccgcggcgac gtcgagcccg aggtgccggc ggatggcatg gagcgtccgg
     4081 tcgccgcgcg cgtcggcgac gccgggcttg aggcccagtt cgaggcgatg caggcggccg
     4141 gcggccggcg cggtcgcggt gggggcggca ggcgtcatga agtttcgtcc cggcaggaaa
     4201 tccgccgacc gatcccggcc ggctgcacac gccgccatta aagcaccgcg acgggacgac
     4261 ctgcaacggg cgggacccgg ggccggatca gccgcggggg gacgtgggcc ttgcgtgcgg
     4321 aaccgtgata cctcgatctg gcgctcgcga tggatgacga gaacctgacc cgggcggtga
     4381 cggcgttccc tacgctgcgc gatcggccgc gcgttccggt ttcgctgggc gaactggcgg
     4441 acgaagcggg acctggaatt tgatatgcgg gcgccgaggg tcgggcgc
//
LOCUS       DAOPCO010000321         4482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16036_length_4482_cov_5.028462, whole genome shotgun sequence.
ACCESSION   DAOPCO010000321 DAOPCO010000000
VERSION     DAOPCO010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16036_length_4482_cov_5.028462"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLS90_15595"
     CDS             <1..213
                     /locus_tag="PLS90_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPK03871.1"
                     /translation="SDIDIHAREILKIEEELNRILAEATGQPIERIAADTDRDYFLDA
                     GTAKEYGIIDHVITRKQDDEKAPAKK"
     gene            complement(226..1062)
                     /locus_tag="PLS90_15600"
     CDS             complement(226..1062)
                     /locus_tag="PLS90_15600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPK03872.1"
                     /translation="MSRSGAAAESAQPHSRPWFEVAFQEAYLELYRHRDLQEARRAVE
                     FLGEALALRPEHRLLDLCCGPGRHLLFLARHVRRAFGLDLSRVLLGRAGEHWAALHAA
                     NPRALPAARPMLMQGDMRRLPVADGAVDRVVNLFTSFGYFEDDAENQGVLDEVGRVLS
                     RRRGAEGWFAIDHINRERLLASLRPRSERSLPGGRRVIESRHWDAATRRVVKDVVCVD
                     ASGEEHAWRESVRVYSPTTLERMLAAARLEPVARHGDYDGSAWHEAAPRMILIARRMP
                     EG"
     gene            complement(1059..2408)
                     /locus_tag="PLS90_15605"
     CDS             complement(1059..2408)
                     /locus_tag="PLS90_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK03873.1"
                     /translation="MKRRVLIVDDEKNTREGLRWSLEAAQMEIETAADGNEALERFNR
                     SPFDLVVTDLKMPGMDGLELLKRIKQQDPTVEVIMLTAHGTIETAVEAMREGAFHYQT
                     KPVDLEELRLNCERALKSRDLTRENEELKSLVNRRYGFQNIIGNSPEMERVFQTVRQV
                     APTRATVLIQGESGTGKELIAHAIHFNSPRAKNPFVPVNCGALSATLLESELFGHEKG
                     AFTGAIRTRPGRFEMADGGTILLDEISETTPEFQVKLLRVLQEQVFERVGGVEPIRVD
                     VRVLASTNRDLRKLVDAGRFREDLYYRLNVVTINLPPLRERRDDLPLLIDAFLREFAE
                     ANGRGSVQLAPKARQALQDYDWPGNVRQLRNVIEGIVVMSTGREIALRDLPEEIRSAT
                     PDRGTVTLRVGASLNDAERELIRATLARNGGNRAQTAKVLGIGRKTLYRKMEEYGIE"
     gene            complement(2436..2705)
                     /locus_tag="PLS90_15610"
     CDS             complement(2436..2705)
                     /locus_tag="PLS90_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03874.1"
                     /translation="MRRLTPAILVGLLLLARLAGAGEATDGIELIWIEPSEGKDALPD
                     LPIFAEVPPKEFPGRFIRYQLRKAVDPRVSIDVNATPGLCSCPPN"
     gene            complement(2747..4051)
                     /locus_tag="PLS90_15615"
     CDS             complement(2747..4051)
                     /locus_tag="PLS90_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03875.1"
                     /translation="MDNPGFEDKLLSRLGKLDPDQVQSYVTRVLSQRQFLLKIFDHLD
                     EGIIVTDRELTLLFMNRRARTMMGWPRNRSVLGEPIDERLDPAHPLHECLGSLRGRLR
                     AIDGFECSYGRDRVLSLSTLPMRSAGSGEFGDEEPEELLIILLHDITERKEREFEQAR
                     AKRLASLATLTSGIAHEIKNPLNALNIHAQLLHAEVRLARAEGRSPDLDKTERAVDVM
                     LEELHRMGRTIEEFLQAARPRSPQLESRDLRPVLESARRIYDPECEERGVQFDLRVDP
                     DLPPVMIDEHLMLQVLRNLLSNALDAMKVRAEQARETNEHYVPRLSVEARLAGDAVKL
                     TITDNGTGIPEEMIEKISEPFFTTKFGGSGLGLMVVYRIIAEHRGALDVETHPGEGTR
                     FTISLPLHIRPVRLLESGKDQFQPPEIRIVDPSLVGDRSGHT"
     gene            4406..4481
                     /locus_tag="PLS90_15620"
     tRNA            4406..4481
                     /locus_tag="PLS90_15620"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4439..4441,aa:Ala,seq:cgc)
BASE COUNT          719 a         1606 c         1405 g          752 t
ORIGIN      
        1 tccgacatcg acatccacgc ccgcgaaatc ctcaagatcg aggaagagct caaccggatt
       61 ctggcggagg ccaccggcca gccgatcgag cggatcgccg ccgacaccga tcgcgactac
      121 ttcctcgacg ccggcaccgc caaggaatac ggcatcatcg atcatgtgat tacgcgcaaa
      181 caggatgacg aaaaggcgcc ggcgaagaag tgacggtcgg ggaccctacc cctccggcat
      241 ccggcgcgcg atgaggatca tccgcggcgc ggcttcgtgc caggccgagc cgtcgtagtc
      301 gccgtggcgg gccaccggtt ccagccgcgc ggccgccagc atccgctcca gcgtcgttgg
      361 cgaatagacc cgcaccgatt cacgccaggc gtgctcctcg cccgaggcgt ccacgcaaac
      421 gacgtccttg accacgcgcc gcgtcgccgc gtcccagtgc cgcgattcga tcacgcgccg
      481 cccgccgggc aggctgcgtt cggagcgcgg ccgcaggctc gccagcaacc gttcgcggtt
      541 gatgtgatcg atcgcgaacc agccctcggc gccgcgccgc cggctcagga cccgccccac
      601 ctcgtccagc acgccctggt tctccgcatc gtcctcgaaa taaccgaaac tcgtgaacag
      661 attcaccacg cggtcgaccg cgccatcggc gaccggcagc cggcgcatgt cgccctgcat
      721 caacatcggc cgcgcggcgg gcagcgcccg cgggttcgcc gcgtgcagcg ccgcccagtg
      781 ctcccctgcc ctgcccagca gaacgcgcga cagatcgagg ccgaaggcgc gccggacatg
      841 gcgcgcgaga aagagcagat ggcgccccgg tccgcagcac aggtcgagca ggcgatgctc
      901 cgggcgcaaa gccagcgcct cgcccaggaa ctcgacggcg cgccgcgcct cctgcaggtc
      961 gcgatggcga tacagctcga gatacgcttc ctgaaaggcg acttcgaacc acgggcggga
     1021 gtgcggctgg gcggattcgg cagccgcccc ggaacgactc attcgattcc gtattcctcc
     1081 atcttgcggt aaagcgtctt gcgcccgatg cccagcacct tggccgtctg cgcccggttg
     1141 ccgccgttgc gcgcgagcgt cgcgcggatc agctcgcgct cggcgtcgtt gagcgaggcc
     1201 cccacgcgca gcgtcaccgt gccgcggtcg ggcgtggcgg agcggatttc ctcgggcaga
     1261 tcgcgcagcg cgatctcgcg ccccgtgctc atcacgacga tcccctcgat gacgttgcgc
     1321 agttgccgca cgttgcccgg ccagtcgtag tcctgcagcg cctggcgcgc cttcggcgcc
     1381 agttgcaccg agccgcgacc gttggcctcg gcgaactcgc gcagaaacgc gtcgatcagc
     1441 agcggcagat cgtcgcgccg ttcgcgcaac ggcggcaggt tgatcgttac gacgttcagc
     1501 cggtagtaca gatcctcgcg aaagcggccg gcgtcgacca gcttgcgcag gtcgcgattg
     1561 gtcgaggcga gcacccgcac gtcgacccgg atcggttcca cgccgccgac gcgctcgaac
     1621 acctgctcct gcagcacgcg cagcagcttg acctgaaatt cgggcgtggt ctcgctgatt
     1681 tcatcgagca ggatcgtccc gccgtcggcc atctcgaacc gccccggccg cgtgcggatc
     1741 gctccggtaa aggccccctt ctcgtggccg aacagctcgc tttccagcag cgtggcgctc
     1801 agcgcgccgc agttgaccgg cacgaacgga ttcttcgcgc gcggcgagtt gaaatgaatc
     1861 gcgtgcgcga tcagctcctt gcccgtcccg ctttcgccct ggatcagcac cgtcgcccgc
     1921 gtcggcgcca cctggcgcac tgtctggaac acccgctcca tttccggcga attgccgatg
     1981 atattctgaa atccgtagcg ccggttgacc agcgacttca gttcctcgtt ctcgcgcgtc
     2041 aggtcgcgcg acttgagcgc ccgctcgcag ttgagccgca gctcctccag gtcaaccggc
     2101 ttggtctggt aatggaacgc cccctcgcgc atcgcttcga cggccgtctc gatcgtccca
     2161 tgcgccgtca gcatgatcac ttccaccgtc ggatcctgct gtttgatgcg cttgagcagt
     2221 tccaggccgt ccatgcccgg cattttgagg tcggtgacga ccaggtcgaa gggggatcgg
     2281 ttgaaccgct cgagcgcctc gttgccgtcg gccgccgttt cgatctccat ctgcgccgcc
     2341 tccaggctcc agcgcagccc ctcgcgcgtg tttttttcgt catcaactat cagcacgcgt
     2401 cgtttcatcg gatcgctatt ccattgaaaa cagacttagt tcggcgggca cgagcagagc
     2461 cccggagttg cattgacatc gatgctcacc cgcggatcga cggccttgcg cagttgatac
     2521 cggatgaaac ggccggggaa ttccttgggg gggacttccg caaagatcgg caggtcgggc
     2581 agcgcatctt tgccctccga cggctcaatc cagatcagtt caatcccgtc tgtcgcctcg
     2641 ccggcgccgg caagtcgcgc gagcagcagc aggccgacca ggatggccgg ggtcagtcgt
     2701 ctcaaggggg ttaacatcgg cgacgcgcca atccttaagg acgttgttaa gtatggccgg
     2761 atcggtcgcc aaccagactg ggatcgacga tacgtatctc cggcggctgg aattggtcct
     2821 tgcccgattc aagcagccgc accggccgga tatgcagcgg caacgagatc gtgaagcgcg
     2881 tcccttcacc cggatgcgtc tcgacatcca gcgcgccgcg gtgctcggcg atgatgcgat
     2941 agaccaccat cagccccaga cccgaaccgc cgaacttggt cgtaaagaac ggctcggaga
     3001 tcttctcgat catctcctcg gggatgcccg tcccgttgtc ggtgatcgtc agcttgaccg
     3061 cgtcgccggc cagccgcgcc tccacgctca accgcggcac gtagtgctca ttggtctcgc
     3121 gggcctgctc ggcgcgcacc ttcattgcgt ccagcgcgtt gctcagcagg ttgcgcagca
     3181 cctgcagcat caggtgctcg tcgatcatca ccggcggcag gtcggggtcg acgcgcagat
     3241 cgaactgaac gccgcgctcc tcgcattcgg gatcgtagat gcgccgcgca ctctcgagca
     3301 ccgggcgcag atcgcgcgat tccaactgcg gactgcgcgg ccgcgccgcc tgcaggaact
     3361 cctcgatcgt tcgccccatc cggtgcagtt cctccagcat cacgtcgacg gcgcgctcgg
     3421 tcttgtccag atcgggcgac cgcccctcgg cgcgcgccaa ccgcacctcg gcgtgcagca
     3481 gttgcgcgtg gatgttgagc gcgttgagcg ggttcttgat ctcgtgcgcg atgcccgagg
     3541 tcagcgtcgc cagcgaggcc agccgcttcg cccgcgcctg ctcgaactcg cgctccttgc
     3601 gctcggtgat gtcgtgcagc aggatgatca gcagctcctc gggctcctcg tcgccgaatt
     3661 cgccggatcc cgccgaccgc atcggcagcg tcgagagact cagcacgcgg tcgcggccgt
     3721 acgagcattc gaagccgtcg atcgcgcgca gccggccgcg cagcgagccc aggcactcgt
     3781 gcagcgggtg cgccggatcc agccgctcgt cgatcggctc gcccagcacg ctgcggttgc
     3841 gcggccagcc catcatcgtc cgcgcccgcc ggttcatgaa cagcagcgtc agctcccggt
     3901 cggtgacgat gatcccttcg tccaggtggt cgaagatttt gagcagaaac tgccgctgcg
     3961 acaggacccg cgtcacatac gactgcacct ggtcggggtc cagtttcccc agccgcgaca
     4021 ggagcttgtc ctcaaatccg gggttgtcca tcggccgttg accgttctcg ccggccctcg
     4081 ccggcgcaga tgactcacgg gcccattttg agccagtcgc gggattgcgt caaggcgggg
     4141 gacgaccgcg cgccgatccg gcggaccaac gggccagggc gcggcgcaag tcagccgcgc
     4201 cacgtgttcc aaaaacacgc agctcctccc cgcgcccgcc cacgggggag tcagctcggc
     4261 ctccaacgca tccgcgacca tccggtcgcg ctcctccccg cgcccgccca cgggggggtc
     4321 aggatctacc tgcccgtgcc ctgttggacg caacttgccg cttgccaagc gacccgccgg
     4381 gcaagtataa gaggggggtc cgcgtgggcc tgtagctcag ctgggagagc gccgcgttcg
     4441 caatgcggag gtcgtcggtt cgatcccgtc caggtccacc ac
//
LOCUS       DAOPCO010000322         4473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16071_length_4473_cov_7.634224, whole genome shotgun sequence.
ACCESSION   DAOPCO010000322 DAOPCO010000000
VERSION     DAOPCO010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16071_length_4473_cov_7.634224"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLS90_15625"
     CDS             complement(<1..285)
                     /locus_tag="PLS90_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPK03876.1"
                     /translation="MKTFRQALAEKKFLLGTFLQIPAADSAEIAGHAGMDFGIVDTEH
                     GTFGADSAIDLVRGCDTAGMASVFRVPRLDHHRIGQALDFGASAVMVPNLT"
     gene            complement(298..1335)
                     /locus_tag="PLS90_15630"
     CDS             complement(298..1335)
                     /locus_tag="PLS90_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPK03877.1"
                     /translation="MSGAYFSRVAQSTPTEFWINNATPAQARQAMELGAVGATTNPTY
                     LAKIAPDDEELLARLDELIDRDVAPEAAIMEVYQGSVLRLASIFRPLFEASGGRHGLV
                     AIQGNPRRNDDAEFMIREGLALAALAENIIVKVPATPAGAAAMQALVRENIPTIATLG
                     FSVAQAVYMAEAYERARAAADKSPRCYVTYIAGILDDVLAAEAREQHLPLDEAALRAA
                     GCEGTRIAYEEYRRRGFTARLMGGGARGPHHFSELAGGDLAITIGWNLTEALQHEAPA
                     PRIDRHAAAETVAALRAHLPDFVKAADPDALSPAEFTRFRPVREFQASFVKAFDDLER
                     FIAGRKTRQHV"
     gene            complement(1385..2152)
                     /locus_tag="PLS90_15635"
     CDS             complement(1385..2152)
                     /locus_tag="PLS90_15635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03878.1"
                     /translation="MNLDFEGKTVVLTGAAGGIGRACVEAFRREGANVLAADIDPAGA
                     AIAERHAPAVVFHPTDLAASQSIQAMIAAAVDAFGRIDVLVNNAALIEPMLPVHETPE
                     ADFDRLIAVNLRGPFLCCKYAHPHLRAARGSIVNVSSMAGVAGEASHAAYCAAKGGLD
                     ALTRAMAIDYGRDGIRCNSVCPSSVLTPATDAMIRRQPNAEAIVEYRRRINQLGYTAT
                     PEEIARVIVFVASPAASFLTGAVIPVSGGSECGYGLK"
     gene            complement(2149..3666)
                     /locus_tag="PLS90_15640"
     CDS             complement(2149..3666)
                     /locus_tag="PLS90_15640"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HPK03879.1"
                     /translation="MARPCIAIGQLSLESNSFVSTVSGLDHFRETGYLLGGDELFGLR
                     GGHGEVAGMLDALAPAGVDVLPLLAARGNSTGLIAGDCYRQLKHSMLENLRNGRAVDG
                     VLLSCHGSMVIEDIDDPEGDLAAAVRAAVGRDVPVIMTLDLHANVTRRMVTELDALVA
                     YRQYPHDDTFEVGQRAARLLLKILAEGIRTRVGYAPLPMLLTAFNATTKDDGPFARLV
                     QRAAALEEDPEILSASVFFVGSYIDVEEIGCSVVVVADANQEKADRAARELAEYYWSL
                     REAFHVVPLSVAEAVERGRAIDGQPILLLDTADTTGGGGAGDGIGLVRELLRLGVTEP
                     CYATVVDPAAARRCAEAGVGARLTLQVGHAIDPRWGEPLTLDGAVKRICDGRFTYDGG
                     ILGGQTVSLGLTAVFSHPPIELVIMSVATYDWGDEKLRTVGLDPRQAKFIGVKNMMNF
                     RFGYRDVMRGYFLLDIPGPTPCDMRMLKFKRIPAAIYPFDEELADPFVEELSIRG"
     gene            complement(3718..>4473)
                     /locus_tag="PLS90_15645"
     CDS             complement(3718..>4473)
                     /locus_tag="PLS90_15645"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPK03880.1"
                     /translation="EELVEEALRLRERGFSRFKTRLGKNWDYDTSAVRRVSAAIAPWG
                     RLLVDGSHRYDLETAVRFDDVLAEHDVFWFEEPFPPEELDRYAALRPRLKTPLAAGEN
                     DFGLQGFRELMRAGAVDIVQADACRAGGITELRRIIAMAAEHQLKFAPHTWSDAVALV
                     ANAHLAASTPLGLTVEVDQTGNPFIEELLCEPLTIRDGQLLLGDRPGLGVEVDPAALA
                     RLTLPRGQTIPPGNYSDLIYQPEYAYEAPPYAG"
BASE COUNT          718 a         1489 c         1580 g          686 t
ORIGIN      
        1 ggtcaggttg gggaccatca cggcggaggc gccgaaatcg agagcctggc caatgcggtg
       61 atgatcgagc cgggggacgc ggaagacgct ggccatgccg gcggtgtcgc agccgcgcac
      121 cagatcgatg gcggaatcgg cgccaaaggt gccatgttcg gtgtcgacaa tgccgaagtc
      181 catgccggcg tggcccgcga tctcggcgga gtcggccgcc gggatctgga gaaacgtccc
      241 cagcaggaat ttcttttcgg cgagcgcctg tctgaaggtt ttcatggcgt ttcctctcta
      301 cacatgctgt cgcgtcttgc gcccggcgat gaaccgctcc agatcgtcga aggccttgac
      361 gaaggaggcc tggaactcgc gaaccggtcg gaaccgtgtg aattcggcgg gcgagagcgc
      421 gtccgggtcc gcggccttca cgaaatcggg cagatgcgcg cgcagcgcgg cgaccgtttc
      481 cgcggcggcg tgccggtcga tccggggggc cggcgcctca tgctggagcg cctcggtcag
      541 gttccagccg atcgtgatgg ccaggtcgcc gccggcgagt tcggagaaat gatgcggccc
      601 gcgggcgccg ccgcccatca ggcgcgccgt gaacccgcgc cggcgatact cctcataggc
      661 gatgcgcgtc ccctcgcagc cggcggcgcg cagtgcggcc tcgtcgagcg gcaggtgctg
      721 ttcgcgcgcc tcggccgcca gcacgtcgtc gagtatcccc gcgatgtaag tcacgtagca
      781 gcggggggac ttgtcggcgg cggcgcgcgc gcgctcatag gcctcggcca tgtacacggc
      841 ctgggcgacc gaaaagccga gcgtggcaat ggtcgggatg ttctcgcgga cgagggcctg
      901 catcgccgcc gcgccggcgg gggtggcagg cactttgacg atgatgtttt cggccagcgc
      961 cgccagcgcg agcccctcgc ggatcatgaa ctcggcgtcg tcgttgcggc gcgggttccc
     1021 ctggatcgca accagcccgt ggcgaccgcc cgaggcttcg aacagcggcc ggaagatcga
     1081 agccaggcgc aggaccgacc cctgataaac ctccatgatc gcggcttcgg gcgcgacgtc
     1141 ccggtcgatc agctcatcca ggcgggcgag gagttcctcg tcgtcgggcg cgatcttcgc
     1201 cagataggtc gggttcgtcg tggccccgac ggcgcccagt tccatggcct ggcgggcctg
     1261 cgcgggggtg gcgttgttga tccagaactc cgtcggcgtg gactgggcga ccctgctgaa
     1321 atacgcgccg ctcatcggac gttcctcctg gccggcgaga gagccggccg gcaaacaggc
     1381 attgttattt gaggccgtag ccgcattccg aaccgcccga gacggggatg acggcgccgg
     1441 tgaggaagct ggccgccggc gaagcgacaa acacgatcac ccgcgcaatc tcctcgggcg
     1501 tcgccgtgta gcccagctga ttgatccggc ggcgatattc cacgatcgcc tcggcgttcg
     1561 gctggcgccg gatcatggcg tcggtggccg gcgtcaatac gctggacggg cagaccgagt
     1621 tgcagcgaat gccgtcgcgg ccgtagtcga tcgccatcgc gcgggtcagg gcgtcgagcc
     1681 cccccttggc ggcgcagtag gcggcatggc tcgcctcgcc ggcgaccccc gccatcgacg
     1741 agacgttgac aatcgagccc cgcgcggcgc gcaggtgggg atgggcatac ttgcagcaca
     1801 ggaacggccc ccgcaggttg accgcgatca ggcggtcgaa atccgcctcc ggcgtctcgt
     1861 gcaccggcag catcggctcg atcagcgcgg cattgttgac caggacgtcg atccggccga
     1921 aggcgtcaac ggccgcggcg atcatcgcct ggatgctctg gctcgcggcc aggtcggttg
     1981 ggtgaaagac gacggccggc gcgtggcgct cggcgatggc cgcgcccgcc gggtcgatgt
     2041 cggccgccag cacgttcgcg ccctcccggc ggaaggcctc cacgcaggcg cggccgatcc
     2101 cgccggcggc gcccgtcagc accacggtct tgccttcgaa gtcgaggttc atccgcggat
     2161 cgacagctcc tccacgaagg gatcggcgag ttcctcgtcg aacgggtaaa tcgccgccgg
     2221 aatgcgcttg aatttcagca tccgcatgtc gcagggcgtg ggccccggaa tatccaatag
     2281 aaaatagccg cgcatcacgt cgcggtagcc gaagcggaaa ttcatcatgt tcttgacgcc
     2341 gatgaatttc gcctggcgcg ggtccagccc gacggttcgc agcttctcgt cgccccaatc
     2401 gtaggtcgcc accgacatga tgaccagctc gatcggcgga tggctgaaga ccgccgtcag
     2461 gcccaggctg accgtctgac cgccgaggat gccgccgtcg taagtgaagc ggccatcgca
     2521 gattcgcttg accgcgccgt ccagggtgag gggctcgccc cagcgcggat cgatggcgtg
     2581 acccacctgc aaagtcaatc gggcgccgac gcccgcctcg gcgcagcggc gcgccgccgc
     2641 ggggtcgacc acggtggcgt agcacggctc ggtgacgccg aggcggagca gttcgcgcac
     2701 gaggccgatg ccgtcgcccg cgccgccgcc gccggtcgtg tccgccgtat cgagcagcag
     2761 gatcggctga ccgtcgatcg cgcggccgcg ctcgaccgct tcggcgacgc tgagcggaac
     2821 gacgtggaac gcttcgcgca gcgaccaata atactccgcc agctcgcggg ctgcgcgatc
     2881 cgctttctcc tggttggcgt cggcgacgac gaccacgctg cagccgattt cctccacgtc
     2941 gatataggag ccgacgaaga agaccgaggc cgagaggatt tccggatctt cctcgagcgc
     3001 ggccgcccgc tggacgaggc gggcgaaggg cccgtcatcc ttcgtcgtcg cgttgaaggc
     3061 ggtcagcagc atcggcagcg gcgcatagcc cacgcgcgtc cggatgccct cggcgaggat
     3121 cttgagcagc agccgcgccg cgcgctggcc cacctcgaac gtgtcgtcgt gcgggtactg
     3181 ccggtaggcg acgagcgcat cgagttcggt caccatccgg cgcgtgacgt tggcgtgaag
     3241 atccagcgtc atgatcaccg gcacgtcgcg gccgaccgcc gcgcggaccg ccgcggcgag
     3301 atcgccctcc ggatcgtcga tatcctcgat gaccatcgaa ccatgacagg agaggagcac
     3361 gccatcgacg gctcgcccgt tccgcaggtt ctccaacatg ctgtgtttga gctgccggta
     3421 gcagtcgccg gcgatcaggc cggtcgaatt gccccgcgcg gccagcagcg gcagcacgtc
     3481 cacgccggcc ggcgccagcg cgtcgagcat gccggcgact tcgccgtggc cgccgcgcag
     3541 cccgaacagt tcgtcgccgc cgagcaggta gcctgtttca cgaaaatgat ccaagccact
     3601 gacggtcgag acgaaactat tggattcaag cgacagttgg ccgatggcga tacagggccg
     3661 agccatggga attacctttc ggagccgggg gcgaggtctg atggggcgcg cgggagatca
     3721 accggcgtac ggcggcgctt cgtaggcata ctccggctgg tagatcaggt cggagtaatt
     3781 gcccggcgga atcgtctgcc cgcgcgggag cgtgaggcgc gccagcgcgg cgggatcgac
     3841 ttccacgccc aaccccggcc ggtcgcccag gaggagctga ccgtcgcgaa tcgtcagcgg
     3901 ctcgcagagc aattcctcga tgaatggatt gccggtctgg tccacctcga cggtcaggcc
     3961 gagcggggtc gaggcggcca gatgggcatt ggcgacgagg gcgaccgcgt cgctccaggt
     4021 gtgcggggcg aacttgagct ggtgttcggc ggccatggcg atgatccggc gcagttcggt
     4081 gatgccgccg gcgcggcagg catcggcctg cacgatgtcg acggcgccgg cgcgcatcag
     4141 ctcgcgaaag ccctgcaggc cgaaatcatt ctcgccggcc gccaggggcg tcttgaggcg
     4201 cgggcgcagc gccgcgtagc ggtcgagctc ctcggggggg aacggctcct cgaaccagaa
     4261 gacatcatgc tcggcaagca cgtcgtcgaa gcggacggcc gtttccagat cgtagcgatg
     4321 cgaaccatcg accaggagcc ggccccaggg ggcgatcgct gccgagacgc ggcgcacggc
     4381 ggacgtgtcg tagtcccagt tcttgcccag gcgcgtcttg aagcggctga aaccccgctc
     4441 gcgcagccgc agcgcctcct cgacgagttc ctc
//
LOCUS       DAOPCO010000323         4445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16204_length_4445_cov_6.869932, whole genome shotgun sequence.
ACCESSION   DAOPCO010000323 DAOPCO010000000
VERSION     DAOPCO010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16204_length_4445_cov_6.869932"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1396)
                     /locus_tag="PLS90_15650"
     CDS             complement(50..1396)
                     /locus_tag="PLS90_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03881.1"
                     /translation="MKLQDATGETAGLSRRQFLASAAAAGAGLVLARPVFAQQANLNS
                     ALQVAFIGVGSQGANLLNNCLKIDGVRIIAICDIWQYQRERFSKIMEKYNQPVNVYED
                     YQEMLASEPGIDAVIVATPDFCHAQQTNDCLRAGKHVYCEKEMARTLEEAASMVRTQR
                     ETGKLLQIGHQRRSNPRYGHALKLIENDKILGRITHFNGQWNRPVAEPYDWPKGSEIP
                     EETLARYGFKNMHEYRNWRWYKAYGSGPFADLGSHQLDVFNWFLKTTPASVMASGGID
                     YYTDGREWYDNVLAIYTYETPAGLVRGVYNVYNTTGHGGFFETFMGDEGSMVISEDER
                     KGYFFREVHAKKREWEEQSETVDKGGEEAFELKIGETLAPDGSPEPEGQKLLEESKKP
                     VHQLHLENFFKAVRGEEELNCPPEIGYETAVTVLRVNDAVASGGTLTFAPEDFTVA"
     gene            1659..1865
                     /locus_tag="PLS90_15655"
     CDS             1659..1865
                     /locus_tag="PLS90_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010488136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPK03882.1"
                     /translation="MPDKVYKKIEVVGSSADSVSDAVKVGLARAGETVRNIDWFEVTE
                     IRGAVQAGKPIYQVTMKIGFRLEA"
     gene            1900..3255
                     /locus_tag="PLS90_15660"
     CDS             1900..3255
                     /locus_tag="PLS90_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK03883.1"
                     /translation="MNALQASRILLADDDPNFRKVVSFNLTRQGAEVLPAENGRQAWD
                     LFRRERPDLVLSDIRMPELDGLALLREIHAVAPDLPVILITAHGDIDMAVEAMQAGAA
                     DFVTKPFDRERLLRKLERALRPVQLEKENRILREELTTRHSFDSIIGAGPAMTRLFEI
                     MRRVLPRETTVLILGESGTGKELVARALHYNGPRRNGPFVAVNCAAIPVNLLESEMFG
                     HTRGAFTGADTAREGRFQLASGGTLFLDEIGDMPPELQAKLLRVLQERQVEPVGSSTP
                     VAVDVRLIAATHQDLEQLVRDGRFRQDLYYRLNVVPITVPALRDRREDIPLLVRHFLR
                     RLGEDGLEVEHDAMERLSRLNWPGNVRELENTIERALALRRHPDRITADDIQPYPGSA
                     PAAANLYEVPDEGLVLDELEKALIQSALRKTGNNQTRAAALLGITRQKLIYRMQKHDL
                     A"
     gene            3242..4390
                     /locus_tag="PLS90_15665"
     CDS             3242..4390
                     /locus_tag="PLS90_15665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03884.1"
                     /translation="MIWPEPRRAAWVRGGVIAALTAALIAIHFATPATPAAMHWHALH
                     RHLLYLPIILAAFWFGWPGGGVLALVVSLLYFPHLVPGDAAGAHAHHPPAGPSLSSMW
                     LLDSINYLWVGILTGWLAQRLENANRDLRRQSAQLRTAMEDLRVRTREVFAAEEQLRR
                     ADRLTALGQLTAGLAHEIRNPLGSIRGAAEIMADPDLSQAQREEFSRVMMEETERLDK
                     VLASFLAYARDQRQGEDEACQVAAAVERTLTLLDSRLHRAGVQVELDLEPECPSLAMP
                     EALLQQVLLNILLNSIQAMPDGGRIVIHSRRDGDRCTLTVTDSGTGIPPEVRPRVFDP
                     FFTTKPSGTGLGLAIVHKIVTGQRGTVDLDPAMERGARVILSLPAVDA"
BASE COUNT          716 a         1627 c         1316 g          786 t
ORIGIN      
        1 cgcattaagt gtcgtctctc gtccctcgtc ttcgtgatcg gtcgtagtcc taggccaccg
       61 tgaaatcctc cggtgcgaac gtcagtgtcc cgcccgacgc taccgcgtcg ttgacccgca
      121 gcaccgtcac ggccgtctcg tagccgattt ccggcggaca gttcagctcc tcttccccgc
      181 gcacggcctt aaagaagttc tccaggtgca actggtgcac cggtttcttc gattcctcca
      241 gcagcttctg gccttccggt tcgggggagc cgtccggcgc cagcgtttcg ccgatcttca
      301 gctcaaacgc ctcctcgccc cccttgtcga ccgtttcgga ttgctcctcc cactcgcgct
      361 tcttggcgtg cacctcgcgg aaaaagtagc cctttcgctc gtcctccgaa atcaccatcg
      421 acccttcgtc gcccatgaac gtctcgaaga aaccgccgtg ccccgtcgtg ttgtagacgt
      481 tatacacccc gcgcacgagc ccggcgggcg tctcgtacgt gtagatcgcc agcacgttgt
      541 cgtaccattc gcgcccgtcg gtgtaataat cgatcccccc gctcgccatc acgctggccg
      601 gcgtcgtctt cagaaaccag ttgaagacgt cgagctgatg cgagccgagg tcggcgaacg
      661 ggccgctgcc gtaggccttg taccagcgcc agttgcggta ttcgtgcatg ttcttgaagc
      721 cgtagcgcgc cagcgtctcc tcggggatct cgctcccctt cggccagtcg tagggctccg
      781 ccaccggccg gttccactgg ccgttgaagt gcgtgatccg gcccaggatc ttgtcgttct
      841 cgatcagctt cagggcgtgc ccgtagcgtg gattgctccg ccgttgatgg ccgatctgca
      901 gcaacttgcc ggtttcgcgc tgcgtgcgca ccatcgaggc cgcctcctcc agcgtccgcg
      961 ccatctcctt ttcgcagtag acgtgcttgc cggcgcgcag gcagtcgttg gtctgctggg
     1021 cgtggcagaa gtcgggcgtc gccacgatca cggcgtcaat ccccggctcg ctggccagca
     1081 tctcctgata atcctcgtag acgttgaccg gctgattgta cttctccatg atcttgctga
     1141 accgctcgcg ctgatactgc cagatatcgc agatcgcgat gatccgcacg ccgtcgatct
     1201 tcaggcagtt gttgagcaga ttggcgccct ggctgccgac accgatgaac gccacctgca
     1261 gcgcgctgtt cagattggcc tgttgcgcaa agaccggtcg cgccagcacc agccccgcac
     1321 cggccgcggc ggccgacgcc agaaattgcc gccgcgacag ccccgcggtc tctccggttg
     1381 catcctgaag cttcatcgag caataactct ccacgatgat tgaccgacac gcgctttgtt
     1441 tatccccgaa cagtgcgcgc aagcgggatt ctaggcaact cgtccgccat tttgcaaagg
     1501 ctaaggcgtt ccgtcccaac tcgtcccgca ctccagaatc gtctgtcgtc cctcgtcctc
     1561 gtcttcgccc ccacgaacct ccggccggcc ttgtccttca ccgcgcgacc cgatatgatg
     1621 agcgtcacct aacctgacag acgatcggag aagccctcat gccggacaaa gtctacaaga
     1681 agatcgaagt cgtcggttcc tcggccgatt cggtcagcga cgcggtcaag gtcggtctgg
     1741 cgcgcgccgg cgaaacggtc cggaacatcg actggttcga agtcacggaa atccgcggcg
     1801 ccgttcaagc cggcaaaccg atctatcagg tcacgatgaa gatcggtttc cggctcgaag
     1861 cctgaccccc cgcgcccggc cgagagaccc cttgacgcca tgaacgccct ccaggcctcc
     1921 cgcatcctgc tggccgacga cgatcccaat ttccgcaagg tcgtctcgtt caacctgacg
     1981 cgccaggggg cggaggtcct gcccgccgaa aacggccgcc aggcgtggga cctcttccgc
     2041 cgcgaacgcc ccgacctcgt cctgagcgac atccgtatgc ccgagctgga cgggctggcc
     2101 ttgctgcgcg agatccacgc cgtcgccccc gacctgccgg tgattctgat cacggcgcac
     2161 ggcgacatcg acatggccgt cgaagcgatg caggccggcg ccgccgattt cgtcaccaag
     2221 cccttcgacc gcgagcggct gctccgcaag ctcgagcgcg cgctccgccc cgttcaactc
     2281 gaaaaggaaa accgcatcct ccgcgaggaa ctgacgaccc gccacagttt cgacagcatc
     2341 atcggcgccg gccccgcgat gacccgcctg ttcgagatca tgcgccgcgt gctgccgcgc
     2401 gagacgaccg tgctgatcct gggcgagagc ggcaccggca aggaactcgt cgcgcgcgcg
     2461 ctgcactaca acggtccgcg ccgcaatggg ccgttcgtcg cggtcaactg cgcggccatt
     2521 ccggtcaacc tgctcgaaag cgagatgttc ggccacacgc gcggcgcctt caccggcgcc
     2581 gacacggcgc gcgaggggcg atttcaactg gcatcgggcg gcacgttgtt cctcgacgag
     2641 atcggcgaca tgccgcccga actgcaagcc aagctgctgc gcgtgttgca ggagcgccag
     2701 gtcgagcccg tcggttcctc gacgccggtc gccgtcgatg tgcgcctgat cgccgccacg
     2761 caccaggacc tggaacaatt ggttcgcgac ggccgcttcc gccaggatct gtattaccgc
     2821 ctcaatgtgg ttcccatcac cgtccccgcg ctgcgcgacc gccgcgagga catccccctg
     2881 ctggtgcgcc atttcctgcg ccggctcggc gaggacgggc tggaggtgga acacgacgcg
     2941 atggaacgcc tctcgcgcct gaactggccg ggcaacgtgc gcgaactgga aaacacaatc
     3001 gaacgcgccc tcgccctgcg ccgccatccc gaccggatca cggccgacga catccagccc
     3061 taccccggtt cggcccccgc cgccgccaac ctttatgaag tgcccgatga ggggctcgtg
     3121 ctggacgaac tggagaaggc cctgatccag agcgcgctgc gcaagaccgg caacaaccag
     3181 acgcgcgccg ccgcgctgct cggcatcacc cgccagaaac tgatctaccg gatgcagaag
     3241 catgatctgg cctgagccgc gccgcgcggc ctgggtccgc ggcggggtca ttgccgcgct
     3301 gacggccgcg ctgatcgcaa tccacttcgc cacccccgcc acgccggcgg cgatgcactg
     3361 gcacgccctc caccgccatc tgctctacct gccgatcatc ctggcggcgt tctggttcgg
     3421 ctggcccggc gggggcgtgc tggcccttgt ggtcagcctg ctgtatttcc cccacctcgt
     3481 cccgggcgat gcggccggcg cgcacgcgca tcatccgccg gccgggccgt cgctctcgtc
     3541 gatgtggctg ctcgattcga tcaactacct ctgggtcggc atcctgacgg gctggctggc
     3601 ccagcggctg gagaacgcca accgcgatct gcggcgccag tccgcgcagc tgcggacggc
     3661 gatggaggac ttgcgcgtcc gcacgcgcga ggtgttcgcg gccgaggaac agttgcgccg
     3721 cgccgatcgc ctgacggcgc tgggacaact gaccgccggc ctggcccacg aaatccgcaa
     3781 cccgctcggc tcgattcgcg gcgccgccga gattatggcc gatcccgatc tctcccaggc
     3841 ccagcgcgag gaattcagcc gcgtcatgat ggaggaaacc gaacggctcg acaaagtcct
     3901 cgccagtttc ctggcctacg cccgcgatca gcgccagggg gaggacgagg cctgccaggt
     3961 cgccgccgcg gtcgagcgca cgctcacgct gctcgactcg cgcctgcacc gcgccggcgt
     4021 tcaggtcgag cttgacctgg aacccgaatg ccccagcctg gccatgcccg aggcgctgct
     4081 ccagcaggtc ctgctcaata tcctgctcaa ctcgatccag gccatgcccg acggcggccg
     4141 gattgtcatc cacagccgcc gcgacggcga ccgctgcacc ctgaccgtca ccgactcggg
     4201 aacgggcatc ccgcccgaag tccgcccgcg cgttttcgat ccgttcttca cgaccaaacc
     4261 ctccggcacc ggcctcggcc tcgccatcgt gcataagatc gtcaccggtc agcgcgggac
     4321 cgtggacctc gatcccgcca tggaacgcgg cgcgcgcgtc atcctttccc tgccggcggt
     4381 cgacgcatga acgactactg ggtccatgac ctcgatccct ttctgatccg catcggcgcg
     4441 ggcgg
//
LOCUS       DAOPCO010000324         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16236_length_4438_cov_4.902350, whole genome shotgun sequence.
ACCESSION   DAOPCO010000324 DAOPCO010000000
VERSION     DAOPCO010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16236_length_4438_cov_4.902350"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(285..1667)
                     /locus_tag="PLS90_15670"
     CDS             complement(285..1667)
                     /locus_tag="PLS90_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03885.1"
                     /translation="MLLLLGPLLGLAGGWLNFKWLGLIGGAAAIMWRIAREGGDRPET
                     GLSPREPWTAGAALIAAGLLLSIVFSPVPGLGVWVALREWTLVGLAFFVARHRPTAAE
                     IRFVLGCLIVAMVVQATLALLQFALPAGWRPLLPLHLQAAAGRARMIGTIGNPEYLAT
                     WIAAGGAAAWVWAAALWRSGGARQTGLTLARLAGGGLVIMTAIYLSGGRGALLSAGGA
                     MLPALPLAWRGPVARREGGRSRGGLAIATAAGAGVMLAGLWALIPADERGGTLPARLA
                     EAVDPHSPSIRHRIGLLVVTSRMIVENPVLGTGPGRFTAGFSDTLARSAAEEEGIGFW
                     ALSDALSGTYVGEAHSDALQWWAEYGLAPALGLSLMLAAALAGVAGRLRAGTEGERPD
                     WERPVLLAAWACLAAFGISLATSFPLHGPVRAGGFWVMLGLVSGACCREEMRRNTKQV
                     RRNEVRRREE"
     gene            complement(1806..4142)
                     /locus_tag="PLS90_15675"
     CDS             complement(1806..4142)
                     /locus_tag="PLS90_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HPK03886.1"
                     /translation="MTQPKQDLAGELTRLAQAKELARIEELWLERLDELPTQSAFYKS
                     WIKEMRHAGATERTEELIGLLVDHLLSSDRARPALRILLATIPFYPHSETLRPHLLRA
                     MRGYYREIDNLETLLQLARLDGPDAKEKSLVEGFKAFREWERLTPGQVWQHYDWGEGV
                     VQELDLDARKVRFAFRHDANKEMTVEGARRYLRYLEPTHFLARRAKEPETLARLAETD
                     PVGLIKAVLASQEGSQIKQGELKTLLTHGVMPAEEWNNWWSKAREALKLDPYIDFDAG
                     AGARAVIQLRERPRSFAEEVREQFFAADAGTVVKAELIRQMARKPKETELPDKLLEKM
                     AERLEEEWRLAGDEHPARRLEIAYMLEDLAALRPGGASPRHDAGPLLDQIGEDYTALV
                     ELDHVDYGIRALEALMHRDGEAGCRRAAELLPHAEVKLAQAIWKALDEEQHAELAVAA
                     LQQMLDDPLENPDTYAWAVRAILDGSWGHLEDYFPASAIVQEVLDHMETWHRLAGAEG
                     ADRERAEGARKLLGKMRTLLAAGKYEAISKAVDQAHRDLAARLRAKIADHPALSATFK
                     QQADRVIRVTRPELEGTGTAAMGAGPSIVQKSDELLCTEWAYQKKATELREITAVKIP
                     RNSKVIEEARMEGDLRENAGYQYAKEEQKMLVQTQASLTDMLSRAVIRHARDVDATRV
                     GFGTRIVVQNLDEDVEENYTILGRWEADPEKHVLSMQAPLAMQFSGRREGEELTVERP
                     GGGTTRYRILRIENALATGEWDRPAAPEAEPVKAGEGG"
BASE COUNT          658 a         1885 c         1157 g          738 t
ORIGIN      
        1 atcaccgtaa ttccctctcg atcgcgcttc ccccggcgcc ttcgccgacc ggcggcggtc
       61 gccgtcccgc cggcgggagc ggaaaatccg tctgatccga cggatttcga acccttgaaa
      121 ccgtccgtta ccccgtcccc ttgcccaagg acgaagacgc tcgtggtttt aattcttttt
      181 tgtctcagaa gcaagtaccc aggccgccgg gacgatctca cccatccccg tctctcttct
      241 ctcgtctctc gttctcgtcc tcgtcctcgg ctctcgcaaa cgcactattc ttcccgccgc
      301 cgaacttcat tccttcgaac ttgtttcgta tttcgccgca tctcttcccg gcagcaggcc
      361 ccgctcacca gccccagcat cacccagaat ccccccgcgc gcaccggccc gtgcaacgga
      421 aaactcgtcg ccagcgaaat cccgaacgcc gccagacacg cccaggccgc gagcagaacc
      481 ggccgctccc aatccggccg ctctccctcc gtcccggcgc ggaggcgccc cgcgaccccc
      541 gccagcgccg ccgccagcat cagcgacagc cccagcgccg gcgccagccc atactcggcc
      601 caccattgca gcgcgtcgct gtgcgcctcg cccacgtagg ttccgctcag cgcatcgctc
      661 agcgcccaga acccgatccc ctcctcctcc gccgccgacc gggccagtgt gtcgctgaat
      721 ccggcggtaa accgccccgg cccggtcccc agcaccgggt tttccacgat catccgcgac
      781 gtgaccacca gcaggccgat ccggtggcgg atcgacggcg agtgcggatc gaccgcctcg
      841 gccagccgcg ccggcagcgt gcccccccgc tcatccgcgg ggatgagcgc ccacagcccc
      901 gccagcatca cgcccgcgcc cgccgccgtc gcgatcgcaa gccccccgcg cgaacgcccc
      961 ccttcccgcc gcgcgaccgg cccacgccac gccagcggca gcgccggcag catcgccccc
     1021 cccgcgctca gcaacgcccc gcggccgccg ctcaggtaga tcgccgtcat gatcaccagc
     1081 ccgccgcccg cgagccgcgc gagcgtcaac cccgtttgcc gcgcccctcc ggagcgccac
     1141 agcgccgccg cccacaccca cgccgccgcg ccccccgccg cgatccacgt cgccagatac
     1201 tccggattgc cgattgtgcc gatcatccgc gcgcgccccg ccgccgcctg caggtgaagc
     1261 gggagcagcg gccgccagcc ggcaggcaac gcgaattgca gcagcgccag cgtcgcctgg
     1321 accaccatcg caacgatcag gcagcccagc acaaaccgga tttcagcggc ggtcgggcgg
     1381 tggcgcgcca caaaaaaagc aagacccacc agcgtccact cgcgcagcgc cacccacacg
     1441 cccagtcccg gcacgggtga aaacacaatc gacagcagca gaccggcggc gatcagtgcc
     1501 gccccggccg tccacggctc gcgcgggctc agacccgtct cgggccggtc gcccccctcg
     1561 cgcgcgatcc gccacatgat cgccgccgcg ccgccgatca gccccagcca cttgaaattg
     1621 agccatcccc ccgccagccc gagcaagggc cccagcagca gaagcacgcc ggtcagtcga
     1681 aaggaaatcc gaaccgcggg cggcgcgccg ccggcgcgat ccccgacccg gttggcgcct
     1741 cgctccaatt tcgtccctcg ccctcggctc tcgaaaactc gcgatctgcc gttcgacgca
     1801 gacgttcaac caccctcgcc cgccttcacc ggttccgcct ccggcgccgc cggccgatcc
     1861 cattcgcccg tcgccagcgc attctcgatc cggaggatcc ggtagcgcgt cgttcccccg
     1921 cccggccgct ccaccgtcaa ttcctcgcct tcgcgccgac cgctgaactg catcgccagc
     1981 ggcgcctgca tcgacaggac gtgcttctcc ggatccgcct cccagcgccc cagaatcgtg
     2041 taattttcct cgacgtcctc gtccagattc tgcacgacga tccgcgtgcc gaaccccacg
     2101 cgcgtcgcat ccacgtcgcg cgcgtggcgg atcaccgccc gcgacagcat gtcggtcagg
     2161 ctcgcctgcg tctgcaccag catcttctgc tcttccttcg catattgata accggcgttc
     2221 tcgcgcaagt cgccctccat ccgcgcctct tcgatcacct tggagttgcg cggaatcttg
     2281 accgcggtga tctcccgcag ctcggtcgcc ttcttctgat acgcccattc ggtgcacagc
     2341 agttcgtcgc tcttctggac gatgctcggc cccgcgccca tcgccgccgt ccccgttcct
     2401 tccagctcgg gccgcgtcac ccggatcacg cggtcggcct gctgcttgaa cgtcgccgac
     2461 agcgccgggt gatcggcgat cttggcgcgc agccgcgccg ccaggtcgcg atgcgcctgg
     2521 tccaccgcct tgctgatcgc ctcatacttg cccgccgcca gcagcgtccg catcttcccc
     2581 agcagcttgc gcgccccctc cgcgcgctcc cggtcggccc cctccgcccc cgccagccgg
     2641 tgccacgtct ccatgtgatc gagcacttcc tgcacgatgg ccgaggccgg aaagtaatcc
     2701 tccaggtgac cccacgaccc gtccaggatc gcccgcaccg cccaggcata cgtgtcggga
     2761 ttctccagcg ggtcgtcgag catctgctgc agcgccgcca ccgccagctc cgcatgctgc
     2821 tcctcatcca gcgccttcca gatcgcctgg gccagtttca cctcggcgtg cgggagcagt
     2881 tcggccgcgc gccggcagcc cgcctcgcca tcgcggtgca tcagcgcctc cagcgcccgg
     2941 atgccgtaat ccacgtggtc cagctccacc agcgccgtat aatcctcgcc gatctgatcg
     3001 agcagcggcc ccgcatcgtg ccgcggcgac gccccccccg gtcgcaacgc cgccaggtcc
     3061 tccagcatgt aggcgatctc cagccgccgc gccgggtgct cgtcccccgc cagccgccat
     3121 tcctcctcca gccgctcggc catcttctcc agcagcttgt ccggcagttc cgtttccttc
     3181 ggtttgcgcg ccatctggcg gatcagctcc gccttgacca ccgtccccgc gtcggccgcg
     3241 aagaactgct cgcgcacctc ctcggcgaaa ctccgcggcc gttcccgcag ttggatcacc
     3301 gcccgcgccc ccgcccccgc atcgaaatcg atgtacggat ccagcttgag cgcctcgcgc
     3361 gccttcgacc accaattgtt ccattcctcc gccggcatca ccccatgcgt cagcagcgtc
     3421 ttcaattccc cctgcttgat ctggctgccc tcctggctcg ccagcaccgc cttgatcaga
     3481 cccaccggat cggtctccgc cagccgcgcc agcgtctccg gctccttcgc ccgcctggcc
     3541 agaaagtgcg ttggctccag atagcgcagg taacgccgcg ccccctccac cgtcatctcc
     3601 ttgttcgcat cgtgccggaa ggcgaaccta accttgcgcg cgtccaggtc cagttcctgc
     3661 acgacccctt cgccccagtc atagtgctgc cacacctgtc ccggcgtcag ccgttcccac
     3721 tcgcggaacg ccttgaaccc ctcgaccagg ctcttctctt tcgcgtccgg tccgtccagc
     3781 cgcgccagtt gcagcagcgt ctccagattg tcgatctccc gatagtagcc gcgcatcgcg
     3841 cgcagcaggt gcggccgcaa cgtctcggaa tgcgggtaga acgggatcgt cgccagcaga
     3901 atccgcagcg ccgggcgcgc ccgatcgctg ctcagcagat gatccaccag caggccgatc
     3961 agttcctcgg tccgctcggt cgcccccgca tggcgcatct ccttgatcca cgatttgtaa
     4021 aacgcgctct gcgtcggcaa ttcgtccagc cgctcgagcc acagctcctc gatccgcgcc
     4081 agctccttcg cctgcgccag ccgcgtcagc tcacccgcca gatcctgttt cggttgcgtc
     4141 atcgttcctt catcccgatc aatttcgtcc tgcgtcctcg tcctcgtaat cgcccccaac
     4201 ggcccgccca ggcattcgtc ctcgtcctcg aaactcgaaa tcctcgcaac gccactttcc
     4261 tgagtggcca tcctctccac tctctttcct cgtaccgcag ccctccattt catcccgatt
     4321 gccgccaagt ttcaatcgaa actgccccag cccccttgag caacccgtgg ccgctcccaa
     4381 cggtccgccc caaaattcgg ctctcggctc gcgtaaagcc cccgacagct cttcccac
//
LOCUS       DAOPCO010000325         4426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16273_length_4426_cov_4.908717, whole genome shotgun sequence.
ACCESSION   DAOPCO010000325 DAOPCO010000000
VERSION     DAOPCO010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16273_length_4426_cov_4.908717"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLS90_15680"
     CDS             complement(<1..872)
                     /locus_tag="PLS90_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK03887.1"
                     /translation="MGTQTMPSSSLEWAKVDLSTTPNVVVPPPGPRSREYHARCTNHF
                     KGLSSQVKLFPVTFESGEGCVLRDVDGNRYIDFSSGIYVTTLGHCHPKVSEAIARYAR
                     TLMNAHDFTTPIKTALVEKLAEVLPGDLSGFQFYDSGTTAVEAGIRVLRAATGKNEML
                     SCYYDFHGKTYNAVSLAHIRSQVYGRVRAPGAHMVPRPDPYHPLWTRPDGTIDTDQYI
                     AFYEEYLDKATVGDVAGFVLEPIQGWGGSVIPPDDFFPKLRALCDRLGILLMADEVLT
                     SWGRTGKWLCMEHWG"
     gene            complement(1743..2549)
                     /locus_tag="PLS90_15685"
     CDS             complement(1743..2549)
                     /locus_tag="PLS90_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HPK03888.1"
                     /translation="MPRIPVDARRARIRELLFECETLSVPDLARHFDVSEMTIRRDLD
                     SLAGAGQVRRVHGGALVSERMSFEFDFHERRLRQLPQKQAIAAVAASLIEPGDTLFLD
                     TGTTTLELAQRLKDAAQLTVITTSLAVAATLQFAASVRTILLGGEIRRGSADTSGALT
                     EYLLERLTARVAMVGAEGIDLEGNFYDVDPQLARVAVMMRERSDRTYILCDSRKIGQR
                     ALVRSGHLSEVDALITDWELESDKRAALEALGGVNVTLAGRPAEADRQTA"
     gene            complement(2680..4059)
                     /locus_tag="PLS90_15690"
     CDS             complement(2680..4059)
                     /locus_tag="PLS90_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPK03889.1"
                     /translation="MRLVLGIDLGTTYFKTGVFDPDGVCRGLVRSPVPKVESAECCEL
                     DPDRFWETLRDATHEALRLAGGATGDLQAIGYASQANSFLLLDADDQPLTPLILWPDR
                     RGEGRAPEFVALFNRPDFLAVTGLGFEPMAEFAASKLCWLRRNEPRRWAAAHALQTIS
                     DYLIYSLTGRRVGDASTTALTGLMELETLAWWPAALDQLALDRRLLSQPLRTGHPAGR
                     VTERGAGRLGVPVGAPVAAGGLDHHIAALGAGVGRLASLSESTGTVLACLGLASRPDP
                     RPACCVGPAHLPGQYYRLGSGPDGGAGLDWYQRHFAPELTVAELLHLAGEAPAGCRGV
                     RAARAIQNRSPDEAFNRPPAQRSHGDCVRAILEMQTDALGELIDRLGMGDCRAILATG
                     GAARSDLWLQVKADRLGRTIIRVNTAEPACRGAAMLAACAAGWFSGLDAAGAAWVRAE
                     RVFEPSTSP"
BASE COUNT          686 a         1433 c         1507 g          800 t
ORIGIN      
        1 ccccagtgtt ccatgcagag ccacttgccg gtgcggcccc aactggtcag cacttcgtcg
       61 gccatcagca gaatgccgag gcggtcgcac agggcgcgca gcttggggaa gaagtcgtcg
      121 ggcgggatga ccgagccgcc ccagccctgg atcggctcga gcacgaaccc ggcgacgtcg
      181 cccactgtcg ccttgtcgag gtattcctcg tagaacgcga tatactggtc ggtgtcgatc
      241 gtgccgtcgg gccgggtcca cagcgggtga taggggtcgg gccgcgggac catgtgggcg
      301 cccggggcgc gcacgcggcc gtagacctgc gagcggatat gcgccagcga aacggcattg
      361 taggtcttgc cgtggaaatc gtagtagcac gagagcatct cgttcttgcc ggtggcggcg
      421 cgcagcacgc ggatgccggc ctcgaccgcc gtcgtgccgg agtcatagaa ctgaaagccc
      481 gagaggtcgc cgggcaggac ctcggcgagc ttctcgacca gggcggtctt gatcggcgtc
      541 gtgaaatcgt gggcgttcat cagcgtgcgc gcataacggg cgatcgcctc gctgaccttg
      601 gggtggcaat gcccgagcgt cgtgacgtag atgcccgagg agaagtcgat gtagcggttg
      661 ccgtcgacgt cgcgcaggac gcagccttcg cccgattcga atgtgacggg aaagagtttg
      721 acctggctgg agaggccctt gaagtggttc gtgcagcgcg cgtgatactc gcgcgagcgc
      781 ggcccggggg gcggaacgac cacattgggc gttgtcgaaa gatccacctt ggcccattcc
      841 agcgaactcg acggcatggt ttgcgtcccc atcgtctttc ctccttgcag ttagaattcg
      901 tcgcttgacc tcgttctcgt gctcgctcgg cgctcgaata ttcgttatcg aaccgatcaa
      961 tcggttcttc cgtttggccg atcgtggccg cgggttggat atcgaaccgc cgacgcggtt
     1021 cctgatagtt gggtgtcgtc tgacgtccct acgggactcc ttcgttaatt tgtgtcagtt
     1081 tcccgccact gacgtggcgg gctgacatct gccgtccctg gcgggactgg ccggctggct
     1141 ggcgccggca aagcatcggc cgtttggatc ggtcggttac gtcccttcat tttcaacctt
     1201 gctctgccag aaactcggct cccggctcct cgtccctcgt gctcggcgct cgaatattcg
     1261 ttatcgaatc gatcaatcgg ttcttccgtt tggccgatgg cgaccctggg ttgaatggcc
     1321 gccgggcggc catccaaccc agggtttgga tatcggaccg ctgacgcgat tcctgatagt
     1381 tgggtgtcgt ctgacgtccc tacgggactc cttcgttaat ttgtgtcagt ttcccgccac
     1441 tgacgtggcg ggctgacatc tgccgtccct ggcgggactg gccggctggc tggcgccggc
     1501 aaagcatcgg ccgtttggat cggtcggtta cgtcccttca ttttcaacct tgctctgcca
     1561 gaaactcggc tcccggctcc tcgtccctcg tgctcggcgc tcgaatattc gttatcgaac
     1621 cgatcaatcg gttcttccgt ttggccggtc gtggccgcgg gttggatatc gaaccgccga
     1681 cgcggttccc aagatggcct gatgttcgcc gtccctgacg ggggcggacc cggcgcccgg
     1741 tctcaggcgg tctgccggtc tgcttcggcg gggcggccgg ccagcgttac gtttacaccg
     1801 ccgagcgcct ccagcgcggc gcgcttgtcg gactccagct cccagtcggt aatcagcgcg
     1861 tcgacttcgc tcagatggcc cgagcggacg agcgcgcgct gaccgatctt gcggctgtcg
     1921 cacaggatgt aagtccggtc gctgcgctcg cgcatcatca cggcgacgcg cgccaactgc
     1981 gggtcgacgt cgtagaaatt cccctccaga tcgatccctt cggcccccac catcgccacg
     2041 cgggccgtga gccgctccag caggtattcg gtcaacgcgc ctgaagtatc ggcgctgccg
     2101 cgccggattt cgccgccgag caggatcgtg cggaccgacg cggcgaactg gagcgtggcc
     2161 gccaccgcca gactggtcgt gatcaccgtc aactgcgcgg cgtccttgag tcgctgcgcc
     2221 agctccagcg tcgtcgtgcc ggtgtcgaga aacaaggtgt cgcctggctc gatgagcgag
     2281 gccgcgacgg ccgcaatcgc ctgtttctgc gggagctgcc gcagacggcg ctcgtggaag
     2341 tcgaactcga agctcatccg ctcgctgacg agggccccgc catgaacgcg ccggacctgg
     2401 ccggcgccgg ccagcgaatc cagatcgcga cggatcgtca tctcgctgac gtcgaagtgg
     2461 cgcgccaagt cggggaccga gagggtttcg cactcaaaga ggagctcgcg aatgcgggca
     2521 cggcgggcat cgacggggat tcggggcatg ggataagcgg aaggtcctgg tgttataacc
     2581 tgcgcgttct gccaagaatc tatcattttc tcacacaagg gtcaacggca aaccgaatcc
     2641 gggccaggat gaggcttaaa ggactgattt cagtgggatt tagggacttg tcgagggttc
     2701 gaaaacacgc tccgcacgga cccatgccgc ccccgccgcg tcaagtccgc tgaaccagcc
     2761 ggcggcgcac gccgcgagca tcgccgcgcc ccggcatgcg ggctcggcgg tgttgacgcg
     2821 gatgatggtg cggccgagcc gatcggcctt gacctgcagc cacagatcgc tgcgcgcggc
     2881 gccccccgtc gccaggatcg cgcggcagtc gcccatcccg agccggtcga tcagttcgcc
     2941 cagcgcgtcg gtctgcatct ccaggattgc gcggacgcaa tcgccgtgcg atcgctgcgc
     3001 cgggggtcga ttgaaggctt cgtcgggcga gcggttctgg atcgcgcgcg cggcgcggac
     3061 gccgcggcag ccggccggcg cctcgccggc caggtgcagc agttcggcga ccgtcagctc
     3121 cggggcgaaa tgccgctgat accagtccag cccggctccg ccgtcgggcc ccgaaccgag
     3181 ccggtagtat tgccccggca gatgggcggg gccgacgcag caagcggggc gcgggtcggg
     3241 ccgcgaagcc agccccaggc aggccagcac ggtgccggtc gattcgctga ggctcgccag
     3301 acggccaacc cccgcgccca gcgccgcgat gtgatggtcc agcccgcccg cggcgaccgg
     3361 ggcgcccacc gggacgccga gccggccggc cccccgctcc gtcacccttc cggccggatg
     3421 gcccgttctc agcggctggg agaggagccg ccggtcgagc gcgagctggt cgagcgccgc
     3481 gggccaccag gcgagcgtct ccagctccat caggccggtc agcgcggtgg tgctggcatc
     3541 gcccacgcgc cggccggtga gcgaatagat cagatagtcg gagatcgtct ggagggcgtg
     3601 cgcggcggcc cagcggcggg gctcgttgcg gcgcagccag cagagtttgc tcgcggcgaa
     3661 ttccgccatg ggttcgaagc cgaggcccgt cacggcgaga aaatcgggcc ggttgaacag
     3721 ggccacgaac tccggcgcgc ggccctcccc gcgccgatcg ggccagagga tcagcggcgt
     3781 caggggctgg tcgtcggcgt cgagcaggag gaagctgttg gcctgcgagg cgtagccgat
     3841 ggcctgcagg tcgccggtcg cgccgcccgc cagacgcagg gcctcatgcg tcgcgtcgcg
     3901 gagcgtctcc cagaatcggt cgggatcgag ctcacagcat tcggccgatt ccaccttggg
     3961 aacgggagag cgcacgagcc cgcggcatac gccgtcgggg tcgaagacgc ccgttttgaa
     4021 gtaggtggtg cccagatcaa tccccagcac gagcctcacg gcagcaacct catccgcaaa
     4081 tccaggttac gtatcccgcg tcgtcgtttt cggcgctcga atagtgttgt cgaaccgagg
     4141 aagccgttca ttgattttca gttggtgacc gtgggtggga tatcgaaccg ctgacgcggt
     4201 tcctgattgt caggtgtcgt ctgccgtccc tacgggactc tttcgttgat ttgtgtcagt
     4261 ttcccgccac tgacgtggcg ggctgaagtc tgccgtccct gacgggactg gccggggagc
     4321 gaggccaggg aggcctcctc tattcgaaat ggtcgctcac gatctcatat ttcatcccga
     4381 cgggactggc cggggggcga agccggggag gccccggcca ttcgaa
//
LOCUS       DAOPCO010000326         4410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16346_length_4410_cov_4.840873, whole genome shotgun sequence.
ACCESSION   DAOPCO010000326 DAOPCO010000000
VERSION     DAOPCO010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16346_length_4410_cov_4.840873"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PLS90_15695"
     CDS             <1..621
                     /locus_tag="PLS90_15695"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HPK03890.1"
                     /translation="LLTDRLGWMMALAAAVALISAVAGYLGAHVWNTSVAGMMAVAAG
                     VQFALAALLAPRYGVLGRMLHNLSVGLRIAREDMLTLLWRRGERGREPALAGGAIREL
                     TGDGIVFRLALGGLRRRGLVRVDDGQVALTANGARAAEEIIHAHRLWEVYMADELERR
                     PEAVHEAAHPMEHFLPPELARQIDEHLNHPRTDPHGSRIPRQGRIG"
     gene            complement(1175..2275)
                     /locus_tag="PLS90_15700"
     CDS             complement(1175..2275)
                     /locus_tag="PLS90_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPK03891.1"
                     /translation="MNGGSHRILFIDFWDAPDDPGRVSGPGLALLRDAEVTRLPLRGV
                     DRALLRERIAPALADVDALVVSPWSQFPYPLTAAELDAAPRLRVIAGTFDNRFANWVD
                     LAELARRGITLIDTSRNMTPSVAEFALAMTLNLLRDIPFLIEETRLGRWQPDGHASRP
                     VADFVHGDLSGKRIALAGFGSINRRYAQLAAPFGVRQIACDPNVGDQVLAEYGVERCD
                     SLVELARQAEIFVVGIPPTPATLEIISAEVLDALPRGSLFVLVTRMAVVHQPTLWRRL
                     AGDELRAAIDVFEPEPPPADAWFRRHRHCLPTPHIAGGVFYCHERCFTVACRDAIAVL
                     NGGTAEYQATPRDVALYAGQRVGDATEKMERK"
     gene            2629..3687
                     /locus_tag="PLS90_15705"
     CDS             2629..3687
                     /locus_tag="PLS90_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPK03892.1"
                     /translation="MRKVLVTGSCGLIGSEAVLHYDRLGRDVHGIDNNMRRDFFGDKG
                     DTTWVRRQLSEQAPRYRHHEIDIRDRGALEELFRGETFDLICHCAAQPSHDLSARRPM
                     DDFDVNAVGTLNLLELTRRHCPEAVFICMSTNKVYGDGPNRLRLVEGDTRYDFADAKY
                     QLGISEKFPIDRCLHTPFGASKVAADVMAQEYGHYFGIRTVVFRGGCLTGPRHSGVEL
                     HGFLNYLVKATLEGIPYTVHGYKGKQVRDQIHSADVIAAFDAYAADPKPGAIYNLGGG
                     RENSLSVLEAIQKVEAACRKKLNWTYSPRNRVGDHICYYSDLTRMRRELKGWDVRRSI
                     DTIIAEIVKAQWDRLKRG"
     gene            complement(3729..>4410)
                     /locus_tag="PLS90_15710"
     CDS             complement(3729..>4410)
                     /locus_tag="PLS90_15710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03893.1"
                     /translation="ELIRALPELEPRLALIPLAVRPAAAAPPDYAAARRELGWDDRPR
                     LLTLGRLEWQKGHGLALDALARLTHRAWHYVIGGAGGEAPRLRRQVDRLGLTDRVTFA
                     GWVDEPAKLRLLAGADLFLWPERTHPAFGLAGLEAMLQGTPVLARRRGAAPEVIADED
                     GRRCWIVNEEGTRAVDAWTAALDRLLADPAPLRAARQGLAEATRARFPFQRMIEQVLA
                     EYDRAAGG"
BASE COUNT          746 a         1324 c         1701 g          639 t
ORIGIN      
        1 ctgctgaccg accggctggg gtggatgatg gcgctcgcgg cggccgtggc attgatctcg
       61 gccgtggcgg gctatctggg ggcgcatgtg tggaacacgt cggtggccgg gatgatggcg
      121 gtggcggcgg gggttcagtt tgcgctggcg gcgctgctgg cgccgcgcta cggcgtgctc
      181 ggccggatgc tgcacaacct gtcggtgggg ttgcgcattg cacgcgagga catgctgacg
      241 ctgctatggc ggcgcgggga acgcgggcgc gagccggcgc tggccggcgg ggcgatccgc
      301 gaactgaccg gcgacgggat tgtgttccgg ctggcgctgg gcggcctgcg ccgacgcggg
      361 ctggtgcggg tcgatgatgg tcaggtggcg ctgacggcca acggcgcccg cgcggcggag
      421 gaaatcatcc acgcccaccg tctgtgggag gtctacatgg ccgacgaact ggagcgccgt
      481 cccgaggcgg tgcacgaagc ggctcacccg atggaacatt tcctcccgcc ggagcttgcc
      541 cgccagatcg acgaacactt gaaccacccg cggaccgatc cgcacggcag ccggatcccg
      601 cgccagggcc ggatcggtta ggcccggccc gcggtgactg gcggcactga aacgtgatgc
      661 gggcccaagg tgagatcagt tccgtgaaaa acaagggagg gcgggggccg cagcgcgtca
      721 atataaacag ataaatgaat cttattattg cgtcatgttt cgcgagggcg cgggacctat
      781 ttgggagatt cctccgcgtc tcggcgtctc ggcgtgatgt ccaggcgcgt ccaggaaggg
      841 atggcggctg ccgacgtcaa tgaggtgctg agtcggacgt ggcatgaagc cagaagacga
      901 agaatctcac gccgagaagt ggagacgccg aggcgtggaa ggtggagggg taaggcgggg
      961 aaggagtgga ccgggcgggg cgtgatggcg ggatttcgag agccgaggac gagcgacgag
     1021 agccgggagc cgaattctgg ggaggccgat gggggcgagg ccactcagga gagtggcgct
     1081 ccccggtttc aggcggttgc ttgattccat ctggcgtcgg ggcagcaggg gtcgaggacg
     1141 agaacgtgga cgaaggacga tcccggaagc tcccctactt ccgctccatc ttttccgtcg
     1201 cgtcgccgac gcgctggccg gcgtagaggg cgacgtcgcg cggggtggcc tggtattcgg
     1261 cggttccgcc gttgagcacg gcgatagcgt cgcggcaggc gacggtgaag cagcgttcgt
     1321 ggcagtagaa cacgccgccg gcgatgtggg gcgtgggcag gcagtggcga tggcgacgga
     1381 accaggcgtc ggccggcggc ggctcgggtt cgaagacgtc gatcgccgcg cggagctcat
     1441 cgccggccag ccggcgccag agcgtcggct gatgcaccac cgccatgcgc gtgacgagaa
     1501 cgaagaggct cccgcggggg agggcgtcga ggacctcggc ggagatgatc tcgagcgtgg
     1561 cgggggtcgg cgggatgccg acgacgaaga tctcggcctg gcgggcgagc tcgaccagcg
     1621 agtcgcagcg ctcgacgccg tactcggcca gcacctgatc gccgacgttg ggatcgcagg
     1681 cgatctggcg cacgccgaag ggggcggcga gctgggcgta acgccggttg atgctgccga
     1741 agccggccag ggcgatccgc ttgccggaga gatcgccgtg gacgaagtcg gcgacggggc
     1801 gggaggcatg gccgtcgggc tgccagcgtc ccaggcgggt ctcttcgatg aggaacggga
     1861 tgtcgcgcag caggttgagg gtcatcgcga gcgcgaactc ggcgacggag ggggtcatgt
     1921 tgcgcgaggt atcgatgagg gtgatgccgc ggcgcgccag ctcggccagg tcgacccagt
     1981 tggcgaagcg attatcgaac gtgccggcga tgacgcgcag ccggggggcg gcatcgagct
     2041 cggcggcggt tagcgggtag gggaactggc tccaggggct gacgacgagg gcgtcgacgt
     2101 cggcgagcgc cggggcgatc cgctcccgga gcagggcgcg gtcgacgccg cgcagcggca
     2161 ggcgcgtcac ctcggcgtcc cgaagcaggg cgaggcccgg tccgctgacg cgccccgggt
     2221 cgtccggagc atcccagaaa tcgatgaaca gaatccgatg agacccgccg ttcaagctcg
     2281 ttgttccccc cgagcggaaa gatcgaggcc agcaaacacg attcgatggg cgaatctcaa
     2341 gcggaatcgg aggggggttg agcgcgggag cggccgccat ccagtgatga atgccacgcc
     2401 ggcggtttgt tgttgttctt ggaccggcgg ttgacaggga gggaacaaaa cggcaggtat
     2461 ttcgagagcc gaggatgagg gacaagagct gagagccgaa tcctggggtg ggccgttggg
     2521 tgcgattacg aagacgagga cgagggacgg gaaggggcgg accaaaaggc tcctttcctg
     2581 ttctcacgcc gcgtgataaa accagacatc ccatgcccgg gggcgcgcat gcgaaaggtc
     2641 ctggtcactg gctcgtgcgg actgattggt tccgaggcgg tgctgcatta cgaccggctg
     2701 ggccgcgacg tgcacggcat cgacaacaac atgcggcgcg atttcttcgg cgacaagggc
     2761 gacacgacat gggtccgccg ccagttgagc gagcaggcgc cgcgctaccg gcaccatgag
     2821 atcgacatcc gcgaccgtgg cgcgctggag gagctcttcc gcggggaaac gttcgacctg
     2881 atctgccact gcgcggcgca gccgtcgcac gatctgtcgg cgcgccggcc gatggacgat
     2941 ttcgacgtca acgcggtggg gacgctcaac ctgctcgagc tgacgcggcg ccactgcccc
     3001 gaggcggtct tcatctgcat gagcacgaac aaggtctacg gcgacgggcc caaccggctg
     3061 cggctggtgg aaggcgacac gcgctatgat ttcgccgacg ccaagtatca gttggggatc
     3121 agcgagaaat ttccgatcga ccgttgcctg cacacgccgt tcggcgcaag caaggtggcg
     3181 gccgacgtga tggcgcagga atacgggcat tatttcggga tccgaaccgt cgtgtttcgc
     3241 ggcgggtgcc tgaccgggcc gcgccacagc ggcgtcgagc tgcacgggtt cctcaattac
     3301 ctggtcaagg cgacgctgga gggcattccc tacacggtcc acggctacaa gggcaaacag
     3361 gtgcgcgacc agattcacag cgccgacgtg atcgcggcgt tcgacgcgta tgccgccgat
     3421 ccgaagccgg gggcgatcta caacctgggc ggggggcgcg agaacagcct gtcggtgctc
     3481 gaggcgatcc aaaaggtgga ggcggcctgt cgcaagaagc tgaactggac ctacagcccg
     3541 cgcaaccggg tgggcgatca catctgctat tactcggacc tgacgcggat gcgccgcgaa
     3601 ctgaaggggt gggacgtgcg gcggtcgatt gacacgatca tcgccgagat cgtcaaggcg
     3661 cagtgggacc ggctcaagcg ggggtagctc cgccgcgccc aaaccttccg atgctttcct
     3721 ccgcgcgctc aaccgccggc ggcgcggtca tattcggcga gaacctgctc gatcatccgt
     3781 tgaaagggaa agcgcgcgcg cgtggcttcg gccaatccct gccgggcggc gcggagcggc
     3841 gcgggatcgg ccagcagccg gtcgagcgcg gcggtccagg catcaacggc gcgcgtgccc
     3901 tcctcgttca cgatccagca gcggcggccg tcctcgtcgg cgatgacctc gggcgccgcg
     3961 ccgcgccgcc gcgccaggac cggcgtgccc tgcagcatcg cttcgaggcc ggccagaccg
     4021 aaggcggggt gcgtccgttc gggccagagg aacagatcgg cgccggccag cagccggagc
     4081 ttggccggtt cgtccaccca gccggcgaag gtgacgcgat cggtcaggcc gagccggtcg
     4141 acctggcggc gcagtcgcgg ggcctcgccg cccgcgccgc cgatcacgta gtgccaggcc
     4201 cggtgtgtca gccgggccag ggcgtcgagg gcgaggccat ggcccttctg ccattccagg
     4261 cggcccaggg tgagcaggcg cgggcggtcg tcccagccga gttcgcggcg cgcggccgcg
     4321 tagtccggcg gggcggcggc ggccggccga accgcaagcg ggatgagcgc caggcgcggt
     4381 tcgagctcag gcaacgcgcg gatcaattcg
//
LOCUS       DAOPCO010000327         4403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16373_length_4403_cov_4.663753, whole genome shotgun sequence.
ACCESSION   DAOPCO010000327 DAOPCO010000000
VERSION     DAOPCO010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16373_length_4403_cov_4.663753"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLS90_15715"
     CDS             complement(<1..785)
                     /locus_tag="PLS90_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03894.1"
                     /translation="MHPKTPEIRAQLRNARTLDEKLVLLENAYAGEPCYMLTCGPSIK
                     ETWNDEVAAYLADKLVIAVKQTYDLAPEIVDFQLLNSWNFQPYNYVEPRPIVLMERAD
                     DDPPTPGLQADMLFRIPDPRNFSQRLATTLRFEDWLFTKTLDRPWGPGVVYELGIYLM
                     VHLGVSEIVTMGWDLGELNSPTMEHFFPEEAPESPKSDDILNKPRIRTFEVGDIADST
                     RALYYWLRGRGIHLSIISDRSLVDPVVPRIKHPRDSQSLAHYRT"
     gene            complement(785..>4403)
                     /locus_tag="PLS90_15720"
     CDS             complement(785..>4403)
                     /locus_tag="PLS90_15720"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPK03895.1"
                     /translation="ARDPDRLRLILTRNETREALLADDELFESLWRDPRMLDRIGRDE
                     ALLNMLLVDPRTLERMLNDAGRVRAFVRDPRIQRQMLKDPEAFAALAGMDLLRERIWA
                     NPGLVAALLTDPRALKWVFSSKEVSRALVEDLRTQRRLLADKDLFVALARHGKFIARI
                     WEHPPLVAHLLTDRRAVDWMLTNGDMARRLAQDARLHTRLLGEKAVFEKLAGRERFLE
                     RLWEFPHLIETIVADERSADLMAALPAQAARLARDERLRHALLDDPRAFGELSRADRF
                     IDQLWQQDDLIDRLLNDRRAIEWTEKLPERLAAWARHPAVRERLFADEGAFAELTRRE
                     DFLPRLLASEETLTRLLEREELLSRLARHTPALDRLATRDDLFAWLTGRRDIVQRLLH
                     EDAAQEILLTDRELLGRVLSQPAAQQALLEHDGLLTELLAAPRTIDRLVHDDVLLTRL
                     LEQERVFDKLLSDAPLLQRLLEHERTRDWLLNDRVNRDRLLRDERVLDAIVTDDTGLE
                     RLLNHSRAKERLLSRGELFRSLLQDERAREHAAADRDFLNQLVASTAVADALLANQHA
                     LEQLLRRPALREWIAGRHDYLRELLAVPGVQERLTDDRAWIVGLMREPKALAWLLADA
                     HGLAVIRDRLLARDEVVEDLARDGRIRARLARDGAFLDALFDEPRIAARIAGDENLVA
                     RLLDRRAFVERLLREEPLLEQFAAQEGVLKRIAADPALSSKLLNENEVQERLLADVSL
                     LGKLLGRREAIDLIVGDPSLMLPLARNLRGLEQLMNDPPLLRRILGKGRVLETLVQDA
                     ELLEKVLHHTAALKQLAGDAAAMTRLMGHGVARVWLRSQDWVLEEWLKDRESRQRLIR
                     DPRLREAWLAQPDLPDLLLSQPDVIDCLARSASFVTRLRNHEAVRDTPEQAQTARRLV
                     QFDSLWRRLKPLLAADADPQRAERLDRAREAAGRGEDLSEAMMAVVAPDNRLALASGE
                     LHLPDPRSGWILIHEILLNEELYFETPEEAPRVIDGGTHFGMAIWYFKQRYPNARIIG
                     FEPDPDLRRLARENVRRNGWEDVKIEPHALAGSAGKRDFHRSRRDSMAGSLTERRVLA
                     GDEVDTFAVSCRTLSAYLTEPVNFLKLDIEGLEADVLEEARDHLHQVEQIFCEVHEES
                     GAAGKRLARVLGVLETAGFDVHVGKSHSYQQLTRQRPMKYVGASYSAVLWGRNRHWSR
                     P"
BASE COUNT          725 a         1458 c         1481 g          739 t
ORIGIN      
        1 gtgcggtaat gcgccagcga ctgactgtcg cgcgggtgct tgatgcgcgg cacgacgggg
       61 tccaccaggc tgcggtccga gatgatcgac aggtggatgc cgcggccgcg cagccagtag
      121 tagagcgcgc gggtggaatc ggcgatatcg cctacctcga acgtccggat gcgcggcttg
      181 ttcaggatat catcgctttt cggactttcg ggcgcttcct cgggaaagaa atgctccatc
      241 gtcggcgagt tgagttcgcc caggtcccag cccatcgtca cgatctcgct gacgcccagg
      301 tgcaccatca ggtagatgcc gagttcgtaa accacgccag gtccccacgg ccggtcgagc
      361 gttttcgtga agagccagtc ctcgaagcgc aaggtcgtcg ccaggcgctg gctgaaattg
      421 cgtgggtcgg ggatgcggaa gagcatgtcg gcctgcaagc cgggcgtggg ggggtcatcg
      481 tcggcgcgct ccatgagcac gatcggccgg ggctcaacat agttgtaagg ctggaagttc
      541 caagagttaa gcaattgaaa atcaacaatc tcgggcgcca gatcataggt ctgcttgacc
      601 gcgatgacca gcttgtcggc caggtaggcc gccacctcgt cgttccacgt ttccttgatc
      661 gacggaccgc aggtgagcat atagcacggc tcgccggcgt aggcgttttc cagcaggacg
      721 agcttctcat ccagggttcg cgcgttgcgc agttgggccc gaatttcggg ggtcttcggg
      781 tgcatcaggg acgactccaa tggcggttgc ggccccacag gaccgcgctg taactggcgc
      841 cgacgtattt cattggccgc tggcgcgtca attgctgata actgtgcgat ttgcccacgt
      901 ggacgtcgaa ccccgccgtc tcgagcacgc cgagcacgcg ggccagacgc ttgcccgccg
      961 cgcccgattc ttcgtggacc tcgcagaaga tctgctcgac ctggtgcagg tgatcgcgcg
     1021 cctcctccag cacgtcggcc tccagccctt cgatgtccag cttcaggaaa ttgacgggct
     1081 cggtcaggta ggcgctcagc gtgcggcagg agacggcaaa cgtgtcgacc tcgtcgccgg
     1141 ccaggacgcg ccgttcggtc agcgagccgg ccatcgagtc gcgccgcgag cgatggaagt
     1201 cgcgcttgcc ggccgacccc gccagtgcat gcggctcgat tttcacgtcc tcccagccgt
     1261 tgcgccggac gttctcccgc gccagtcgcc gcaggtcggg atcgggttca aagccgatga
     1321 tgcgggcgtt ggggtaacgc tgtttgaagt accagatcgc catgccgaag tgggtgccgc
     1381 cgtcgatgac gcgcggcgcc tcctccggcg tttcgaaata gagttcctcg ttgagcagga
     1441 tttcatggat caggatccag ccggaacgcg gatccgggag gtgcaactca ccgctcgcca
     1501 gggcgaggcg attatcgggc gccacgacgg ccatcatcgc ttcgctcaga tcctcgcccc
     1561 ggccggcggc ttcacgcgcg cggtccaggc gctcggcgcg ctgcggatcg gcgtcggccg
     1621 cgagcagcgg tttgagccgg cgccagagcg agtcgaattg cacgaggcgg cgcgccgtct
     1681 gggcctgttc gggggtgtcg cgcacggcct catggtttcg gagacgggtc acgaacgatg
     1741 cggagcgggc aaggcaatcg atcacgtcgg gctgactgag gagcaaatcg ggcaggtcgg
     1801 gctgggcgag ccaggcctcg cggaggcggg gatcgcggat cagccgctgg cgcgattcac
     1861 ggtccttgag ccactcctcg agcacccagt cctggctgcg cagccagacg cgggcgacgc
     1921 cgtggcccat cagccgagtc atcgcggccg cgtcgcccgc cagttgtttg agtgccgccg
     1981 tgtggtgcag gactttttcc aggagttccg cgtcctgtac gagtgtctcc agaaccctcc
     2041 ccttgcccag gatgcgccgc aacagcggtg ggtcattcat cagttgctcc aggccgcgca
     2101 gattgcgcgc cagggggagc atgagcgacg gatcaccgac gatcaggtcg atcgcctccc
     2161 gccggccgag cagcttgccc agcagcgata cgtcggccaa cagccgctcc tggacctcat
     2221 tctcgttcag cagtttggag ctcagggccg gatccgcagc gatgcgtttg agcacgcctt
     2281 cctgcgcggc gaactgttcc agcagcggct cttcacgcaa caggcgctcg acgaacgcgc
     2341 gccggtccag caggcgcgcg acgaggttct cgtcgccggc gatgcgggcg gcgattctgg
     2401 gctcgtcgaa aagggcatcg agaaaagccc cgtcccgcgc cagccgagcc cggatgcgtc
     2461 cgtcgcgcgc aaggtcttcg accacctcgt cgcgcgccag gagccggtcg cggatgaccg
     2521 cgagcccatg cgcgtcggcc agcaaccagg cgagggcttt cggctcgcgc atcagcccga
     2581 cgatccatgc gcgatcgtcc gtcagccgct cctgcacgcc gggaacagcc agtagttccc
     2641 ggagatagtc atgccggccg gcgatccact cgcgcagggc cgggcgccgc agcagttgct
     2701 caagtgcgtg ctgattagcg agcagcgcgt cggccaccgc cgtgctggcg acgagctggt
     2761 ttagaaagtc acggtccgct gccgcatgct cccgggcgcg ctcatcctga aggagcgatc
     2821 ggaacagctc gccccgggag agcaggcgct ccttcgctcg cgaatgattc aacaggcgtt
     2881 caagtcccgt gtcatcggtc acgatggcgt ccaacacgcg ctcatcgcgc agcaaccgat
     2941 cgcggttaac ccggtcattg agcagccagt cccgcgtccg ctcatgctcc agcagccgct
     3001 ggagcagcgg ggcatcactg agcaacttgt caaagacccg ttcctgttcg agcaggcgcg
     3061 tcagcaggac gtcatcatgg accaggcggt cgatcgtgcg cggtgcggcc agcagctcgg
     3121 tcaggagccc gtcgtgttcc aacagggcct gctgcgccgc cggttgactc aggacgcggc
     3181 cgagcagttc ccggtcggtc agcaggatct cctgggcggc gtcctcgtgc agcagccgct
     3241 ggacgatgtc gcgccggccg gtcagccagg cgaacaggtc gtcgcgtgtg gcgagccggt
     3301 cgagcgccgg cgtgtgccgc gccagtcggg agagcagttc ttctcgttca aggagccgtg
     3361 tcagcgtctc ctcactcgcg agcagccggg gcagaaaatc ttcgcggcgc gtcagttcgg
     3421 cgaaggcgcc ctcgtcggcg aacagccgtt cgcgcaccgc cgggtggcgc gcccacgccg
     3481 ccaggcgctc aggcagcttc tccgtccact caatcgcgcg gcggtcgttc agcaggcgat
     3541 cgatcagatc atcctgttgc cacagctgat caatgaagcg atcggcgcgc gacagctcgc
     3601 cgaaggctcg cggatcgtcc agcagggcat gccgcagccg ttcatcgcgg gccaggcgcg
     3661 ccgcctgagc aggcagcgcc gccatcagat cggcgctgcg ctcgtcggcg acgatcgttt
     3721 cgatcaaatg agggaattcc cacagccgct cgaggaaacg ctcgcggccg gccagctttt
     3781 cgaagacggc cttctcgccc agcagccgcg tgtgcaggcg cgcatcctgg gccaggcggc
     3841 gcgccatgtc gccgttggtc agcatccagt cgacggcccg ccggtcggtg agcaggtgcg
     3901 ctaccagcgg ggggtgctcc cagatgcgcg cgatgaattt cccgtgtcgc gccaacgcga
     3961 caaacaggtc cttgtccgcc agcaggcgtc gctgcgtgcg caggtcctcg accagcgcgc
     4021 gcgaaacctc tttgctggag aaaacccact tcagcgcacg cgggtcggtc agcagcgccg
     4081 cgaccaaccc cgggttggcc cagatccgct cgcggagcag gtccattccg gccagagcgg
     4141 cgaaggcctc cggatccttg agcatctggc gctgaatgcg cggatcgcgc acgaaggcgc
     4201 ggacgcgacc ggcatcgttc agcattcgct ccagggtgcg cggatcgacc agcaacatgt
     4261 tgagcagggc ctcatcccgc ccgatccgat cgagcatccg cgggtcgcgc cacagcgact
     4321 cgaacagttc gtcgtccgca agcaacgcct cgcgcgtttc gttccgcgtc aggatgaggc
     4381 gcagccgatc gggatcgcgc gcg
//
LOCUS       DAOPCO010000328         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16385_length_4401_cov_5.243442, whole genome shotgun sequence.
ACCESSION   DAOPCO010000328 DAOPCO010000000
VERSION     DAOPCO010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16385_length_4401_cov_5.243442"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="PLS90_15725"
     CDS             complement(<1..1679)
                     /locus_tag="PLS90_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03896.1"
                     /translation="MRLTALFSWMLVPWILCAGTAAEAADMWVEDFKTSSLGFDHPWE
                     EPVVISGTGWAEWTSDGEIGRLALHSEDAYRCWVTTSRSFPWDESTNRFCQIRFSRLT
                     PRARLKLYAISDADGGVPHVILNHYDEAEHPPANELLTVDLRDKLPAGTRSLRVRVEL
                     TKDGSSPPSPTRENPPKGDFRPMPRDIEVYLDWIKLGAPGGEGEPPGAPVAADETPAA
                     FREGEPVTLRWAPPADSPADGYTLICSRDPLFAAACLTELDAVPGTRHTLAAPLAPGT
                     WYWTVSATRAGLSGPFLLDQATGQPPSIVIQRTLSPDERAYDHFSIRGGACSFGSLRH
                     FSDEPILIEQARRMLEMGADAYKFLIGGENYVKCYTDLSDEIREKSRTMVELVQNEPA
                     YKTLLDMPFKYFVMWAYAMKVKLVFGQDQFTEEVAAAEYDQIYEFARWLLQTYAGTGK
                     VFLIGHWEGDNMLMGGATSEVPSEAKIADLIRWHRNRQQAVTDARNSLPDVQGVEVYH
                     YSEVNAISPVLDKDLPRMINAILPHVPVDLISYSAYNCMNHTDELPERAYT"
     gene            complement(1761..4067)
                     /locus_tag="PLS90_15730"
     CDS             complement(1761..4067)
                     /locus_tag="PLS90_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03897.1"
                     /translation="MPTRKTPIAAPWQLAHFHQEDLASADVLSGRARLDWIDAEVPGN
                     VVLDLYRHGLVDDPFMGQNPEKIRPLETHEWWYRTEFTADLPTAGGRQNLYFEGIDTF
                     SEIYLDGKLIATTDNMLIPHRIALPEPLQPGTRHTLHVRIKSDLFEVADRDFEGAVEL
                     VANEPSRIWIRKAGHAHGWNIFPRLLSAGLWGPVHLEFEPDYHIDDVFVFTKKFGRDV
                     SRIMARVFLKTPKVYRTDLSVRIRVLDQGEPVYTETYRMQFPLVVFSFPLENPKLWWP
                     RPYGDPNMYLAEVELLAGDEVLDSWSGRFGVRSVEIIQEHDPVEEHQTFYFQINGVPV
                     SAYGTNHVPLDNFPALSAKREKEFFRLLDESNSNMIRVWGGGIYESSDFYAACDEMGV
                     MVWHDFMYACALYPQDEEFLELVRRETAAVVRRLRNHPSIVLWCGDNEIETHLTHFFS
                     PHQEGGNRAIANQILPEYLTRYDGTRPYWPSSPYTPIPGEPAHSVKAGDTHIWCHGLY
                     YKAPIYSQDLSPFISEIGHLALNNRDSLARFIPAEVLWPYDRIIWGYRCGSLSDPSSY
                     CYPDGRLIQNEMNVRNMFGAVPDNLDDFIIASQIVQAEAYKTWIERARRRKFSCGGIL
                     WWNLLDGCPQVSDAVVDYYYQKKLAFDVIRRVQEDILICLESIEGSLCLFVVSEKLSD
                     CDVNYEVTLDGGVVRSGRIHVPANSSILAETIPADPAKAALYQLRAQVNGSVYTNHYL
                     AGAPLFDLEWLKRQYGTLTGAPSTLANV"
BASE COUNT          758 a         1376 c         1457 g          810 t
ORIGIN      
        1 tgcgtatagg cccgttcggg cagttcgtcg gtgtggttca tgcagttgta ggcggagtag
       61 gagatcagat cgacggggac gtgcggcagg atcgcgttga tcatccgcgg caggtccttg
      121 tcgagcaccg gcgagatggc gttcacttcg gagtagtgat acacctcgac gccctgcacg
      181 tcgggcaggg aattgcgcgc gtcggtcacc gcctgctggc gattgcggtg ccagcggatc
      241 aggtcggcga tcttcgcctc gctcggcacc tccgacgtcg ccccgcccat cagcatgttg
      301 tcgccctccc agtggccgat caggaagacc ttacccgtcc cggcataggt ttgcaggagc
      361 cagcgggcga attcgtagat ctgatcgtat tccgcggcgg cgacttcctc cgtgaactga
      421 tcctggccga agaccagctt gaccttcatc gcgtaggccc acatcacgaa gtatttgaac
      481 ggcatgtcga ggagggtttt gtacgccggc tcgttctgca ccagttcgac catcgtgcgg
      541 cttttttcgc gaatctcatc cgacagatcg gtgtagcact tgacgtagtt ctcgccgccg
      601 atcaggaact tgtaggcgtc ggcgcccatc tcgagcatcc ggcgcgcctg ttcgatcagg
      661 atcggctcgt cgctgaaatg gcgcaggctg ccgaaactgc atgccccgcc gcggatgctg
      721 aagtggtcat aggcgcgctc gtccggcgag agggtccgct gaatgacgat cgaaggcggt
      781 tggcccgtcg cctgatccag caaaaacggc ccggagagcc ccgcgcgcgt tgcgctgacc
      841 gtccaatacc acgtgccggg cgccaggggc gcggccagcg tgtggcgggt ccccggaacc
      901 gcgtcgagct cggtgaggca ggcggcggcg aagagcggat cgcgcgagca gatcagcgtg
      961 tagccgtccg cgggggaatc cgcgggcggc gcccagcgca gcgtgacggg ctcgccttcc
     1021 ctgaatgccg ccggcgtttc gtccgccgcc actggcgccc ccggcggctc cccctcgccg
     1081 ccgggcgcgc ccagcttgat ccagtcgaga tagacctcga tgtcccgcgg cattgggcgg
     1141 aagtcgccct tgggcgggtt ttcccgcgtc ggcgacggcg gcgaactgcc gtccttcgtc
     1201 agctcgacgc gcacccggag gctgcgcgtc cccgccggca gcttgtcgcg cagatccacc
     1261 gtcagcagct cgttggccgg cgggtgttcc gcttcgtcat agtgattcag gatcacgtgc
     1321 ggcacgccgc cgtcggcgtc ggagatcgca tacagtttca accgcgcgcg gggcgtcaac
     1381 cggctgaagc ggatctggca gaagcgattc gtcgattcgt cccaggggaa ggaccggctg
     1441 gttgtaaccc agcagcggta ggcgtcctcg gaatggagcg ccaggcggcc gatctcgccg
     1501 tcggaggtcc attcggccca gcccgtgccc gagatcacga cgggctcctc ccagggatgg
     1561 tcgaaaccca gcgaggaggt tttgaaatcc tcgacccaca tgtcggccgc ctccgccgcc
     1621 gttccagcgc agaggatcca tggaaccagc atccagctga acagcgctgt cagtcgcatc
     1681 acacgcaccc ttccccacgg tgtgttggcg cggcccgggc cgcatcgaag ccggcagacc
     1741 ggatcccgag tgattggcgc ttagacgttg gccaaggttg acggcgcgcc ggtcaacgtc
     1801 ccgtactggc gcttgagcca ttccagatcg aacaacggag cccccgccag atagtggttc
     1861 gtgtagaccg atccgttgac ctgcgcgcgg agctgatata aggccgcctt ggccggatcc
     1921 gcggggatcg tctcggccag gatcgagctg ttcgccggca cgtggatgcg gccggagcgg
     1981 accacgccgc cgtcgagcgt gacttcgtag ttcacgtcgc aatcgctcag cttttcgctc
     2041 accacgaaca ggcagaggct cccctcgatg ctttccaggc agatcaggat gtcctcctgc
     2101 acgcgccgga tcacgtcgaa ggccagcttt ttctggtaat agtagtccac gacggcgtcg
     2161 gagacctgcg ggcagccgtc cagcaggttc caccacagaa tgccgccgca actgaacttg
     2221 cgccgccgcg cccgttcgat ccatgtcttg taggcttcgg cctggacgat ctgcgaggcg
     2281 atgatgaaat cgtccaggtt gtccggcacc gcgccgaaca tgttcctgac gttcatctcg
     2341 ttctggatca gccggccgtc gggatagcag tagctggagg gatcggaaag gctgccgcaa
     2401 cggtaacccc agatgatccg gtcgtagggc cagagcacct ctgccgggat gaacctggcg
     2461 aggctgtcgc ggttgttcag cgccaggtgg ccgatctcgc tgatgaacgg cgacagatcc
     2521 tggctgtaga tcggcgcctt gtaatacagc ccgtggcacc agatgtgggt gtcgccggcc
     2581 ttgaccgagt gggccggctc gcccgggatc ggcgtgtagg gcgacgacgg ccagtagggc
     2641 cgggtgccgt cgtagcgcgt caggtactcc ggcaggatct ggttggcgat cgcccggttg
     2701 cccccctcct ggtgcgggct gaagaaatgc gtcaggtgcg tttcgatctc gttgtcgccg
     2761 caccacagga cgatgctcgg gtggttgcgc aggcggcgca cgaccgcggc cgtctcccgg
     2821 cggaccagct ccaggaattc ctcgtcctgc gggtagaggg cgcaggcata catgaagtcg
     2881 tgccagacca tgacgcccat ctcgtcgcag gcggcgtaga agtcgctcga ttcgtaaatg
     2941 ccgccccccc agacgcggat catgttgctg ttgctctcgt ccagcagccg gaagaactcc
     3001 ttctcgcgct tggcgctcag cgcggggaag ttgtccagcg gaacgtgatt cgtcccgtag
     3061 gcggagaccg gcacgccgtt gatctggaag tagaacgtct ggtgctcttc gaccggatcg
     3121 tgctcctgga tgatctcgac cgagcggacg ccgaaccgtc ccgaccagga atccaggacc
     3181 tcgtccccgg cgagcagttc gacctcggcc agatacatat taggatcgcc atacggccgc
     3241 ggccaccaca gcttggggtt ttccagcggg aagctgaaca ccaccagcgg gaactgcatc
     3301 cgataggttt cggtgtaaac cggctccccc tgatccagga cgcggatgcg caccgacaga
     3361 tcggtccggt agaccttggg ggttttcagg aagacccgcg ccatgatgcg gctgacgtcg
     3421 cggccgaact tcttggtgaa gacgaagacg tcatcaatgt ggtagtccgg ctcgaactcc
     3481 agatggaccg ggccccagag acccgccgag aggaggcggg ggaaaatgtt ccagccgtgg
     3541 gcgtggccgg ccttgcggat ccagatgcgg ctcggctcgt tggcgaccag ttccaccgcg
     3601 ccttcgaaat cccggtcggc gacctcgaac agatcgctct tgatgcggac gtgcagcgtg
     3661 tggcgcgtcc cgggctggag cggctcgggg agcgcgatcc gatgcgggat cagcatgttg
     3721 tcggtcgtgg cgatcagctt gccgtccaga tagatttcgg agaacgtatc gatgccttcg
     3781 aaatagaggt tctgacgccc ccccgcggtc ggcaggtcgg cggtgaattc agtccgatac
     3841 caccactcgt gggtttccag cggacggatc ttttcggggt tctggcccat gaacggatcg
     3901 tcgaccaggc catggcgata gagatcgagg acgacgttgc cgggcacctc ggcgtcgatc
     3961 cagtccagcc gggcgcggcc gctcagaacg tcggccgagg cgagatcctc ctggtggaag
     4021 tgcgccagtt gccaaggcgc ggcgatcggg gtcttgcggg tgggcatgtt atcgagtcac
     4081 tttcgttgtt tcagttggtt gatgtgaaat ggcgcggagc tgtgattgcg catccgcgcg
     4141 gggaaagcag aatgattgac gcgccggggg gaaggcaaca tgccatccgt tcacacggga
     4201 tcgggcgatc tgaatcggtt gcgaggggcc ggcccgccga caggaccggc gccgccacag
     4261 ccctggaacc acccgcgcat ccaatttatc acgcatgata attacagggg ccctcacccg
     4321 ttgtcaatcc ataagcggtc gccggttcgg tgcggttgcc agacaacggc ccagctacaa
     4381 cataaatcaa ttataatcag t
//
LOCUS       DAOPCO010000329         4383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16466_length_4383_cov_4.875231, whole genome shotgun sequence.
ACCESSION   DAOPCO010000329 DAOPCO010000000
VERSION     DAOPCO010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16466_length_4383_cov_4.875231"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="PLS90_15735"
     CDS             complement(<1..868)
                     /locus_tag="PLS90_15735"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPK03898.1"
                     /translation="MALPTVMALLLLTFVFIIGSIRDLIDLVLHPGVTVGQVLVVLAS
                     FLPATVLFVVPMAVLIGVLIGVGRMTLDREVLAMRASGINLFSIFAPMLALAVVLAGV
                     VFWLGAGPVPRMFLRGMERVADMRFALISSLGPGQFHEIEGDDNMALFFRERDPQTQA
                     MKGVVLKMQKNFEGSDLRKKLESLQVSGELKRAVLRVRGGKLELEKPQASDEETTETT
                     RAAAAGDATSVSLTPVLKAGADATPDAERRDAPADAADDEGPEPIFREGSVPIAENEI
                     TYIFAETGGIRTG"
     gene            1424..2572
                     /gene="dnaN"
                     /locus_tag="PLS90_15740"
     CDS             1424..2572
                     /gene="dnaN"
                     /locus_tag="PLS90_15740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPK03899.1"
                     /translation="MKVVFERQSLASSLALVHNVVSSTTTMPILSNVLIEAAGEEVVL
                     SGTDLESFGQVRIKANVEEGGRATASARLLTDIVRLLPDGDVNLETAGNRMTIRAARN
                     TYALTTMPVDDFPDWPKVEPQLTIVLRQADLKRALHNTTFAIPTRDPRKVLMGVLFDV
                     AEGKLTCVATDGRKLGKSIIEPVEIIGGSSIQAILPERILTEIDHAIGEEGEIRIAFA
                     ERQVVFSLSNLTYISNRIEGTYPKYEAVIPQTFKRTIKVQKTILSDAISRAAILAERK
                     HHSIVLHFTNGQIEINARSFEDGSYEGSVETDYDGEPFRIAFNYQYLQEIFKVAPDAV
                     IEMKMKEAAAPVVFECESDPDSLYLIMPVRMTDIDDVPQPAAAGAVED"
     gene            2589..>2832
                     /locus_tag="PLS90_15745"
     CDS             2589..>2832
                     /locus_tag="PLS90_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03900.1"
                     /translation="MNAIGIVPPPRAGQFPPEGGGDGPSAADPAAAPRRFDPWPWVIL
                     GVFAVLSFTMILAMWYGYRIGRFTQKAEALIAAERYA"
     assembly_gap    2833..2842
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2843..3222
                     /locus_tag="PLS90_15750"
     CDS             <2843..3222
                     /locus_tag="PLS90_15750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03901.1"
                     /translation="CYLEMGQPEEALAHLELVRQYAPNEPLDVEYGIAWRLQGKHEEA
                     AESFRKALARKPSDAAAHFYLGLQAQEAGRIAEAATHFQGAAADPKWDRRALPHRQQL
                     ADAVLGDVAPKLEARPPADAKPE"
     gene            complement(3463..4212)
                     /locus_tag="PLS90_15755"
     CDS             complement(3463..4212)
                     /locus_tag="PLS90_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03902.1"
                     /translation="MRLSRPAASSPSAFTLIELLIVVAIIAILAAIAVPNFLEAQIRS
                     KVSRVHSDLRTLATGLEAYRVDHNHYPEGTDNPARYPQQIADFLGNRASGYYTLCTRD
                     GNLIAGRDFHTLTTPVAYATSFPTDPFAEQAAGFMTYCYRPDKEHGTGYILTSFGPDA
                     DLLEDVQGKLGAGTASLNPLSTFMDPGVPARLGDINERGVVYIFEGKPQNLSGPLEPY
                     GGLQGALNDLSYDPTNGSVSDGDIYRLGPGF"
BASE COUNT          765 a         1337 c         1560 g          711 t
ORIGIN      
        1 agccggtgcg gatgccgccg gtttcggcga agatgtaggt gatttcgttc tcggcgatcg
       61 ggacgctgcc ctcgcggaag atcggctccg ggccttcgtc gtcggcggcg tcggccggcg
      121 cgtcgcggcg ttccgcgtcg ggggtggcat cggcgccggc cttcaacacc ggcgtcagcg
      181 agacgctggt cgcgtccccc gcggcggcgg cgcgggtcgt ctcggtcgtc tcctcgtcgg
      241 acgcctgggg tttctccagt tcgagcttgc cgccgcgcac gcgcaggacg gcgcgcttga
      301 gttcgccgct gacctggagc gactcgagct tcttgcgcag atcagacccc tcgaagttct
      361 tctgcatttt caggaccacg cccttcatgg cctgcgtctg ggggtcgcgc tcgcggaaaa
      421 agagcgccat gttgtcgtcg ccctcgatct cgtggaactg gcccgggccg agcgaggaga
      481 tgagcgcgaa ccgcatgtcg gccacccgtt ccatcccccg caggaacatc cgcggcacgg
      541 gtccggcgcc gagccagaag acgacgccgg ccaggacgac ggccagcgcc agcattgggg
      601 cgaagatcga aaacagattg atgccgctgg cgcgcatcgc gagcacttcg cggtcgagcg
      661 tcatccgccc gacgccgatc agcacgccga tgagcaccgc catcggcacg acaaacagca
      721 ccgtggcggg cagaaaactg gccagcacga ccaggacctg gccgacggtg acgcccggat
      781 gcagcaccag gtcgatcagg tcgcggatgc tgccgatgat gaagacgaac gtcagcagca
      841 ggagcgccat gaccgtgggc agagccactt cgcggattga ataacgacgt agaatccagg
      901 ccaacgaggg gaggtccttt ccatgggggg cgccgcgcgg caaggccatt cggccgtcgg
      961 cggccgccat ggcccgacct acgcgcggcg gccgggccga ggccgtcagg attaagccat
     1021 gcgcttcgcc ccaaagtcaa ccggcgaggg ggtccgctcg gttcgcccga tgcgctcgcc
     1081 ggcattctcc cgcatccttg gcatgcgatc cagcgggccg gggggaatgg gcgttgacat
     1141 tcggcgcgcg ctgattagag ttggatgagc cgtcggcgct tcgaaatcgc catgcgggaa
     1201 ggctttcacc cggccagcgc aagtagcccg aaatcaactc gcggcgaacc tgaaaacagt
     1261 cgtggccgtc gctggattga gcccgcggcg tcgccgccgg cggtcggcgg gcggagtgcc
     1321 gagatcgcgc cggcgtcgcg ggccgcggcg ggtcggaccg cgtgggtccg cgggcgcggg
     1381 accgcccata actgaatttg attccacaaa ggaaccgctc gcgatgaaag tcgtctttga
     1441 acgccaatcg ctggccagca gcctggccct cgtgcacaac gtcgtcagct cgacgacgac
     1501 gatgccgatc ctgtccaacg tgctgatcga ggcggcgggc gaggaggtcg tgctcagcgg
     1561 caccgatctg gaatcgttcg ggcaagtgcg gatcaaggcc aacgtcgagg aaggcgggcg
     1621 cgcaacggcc agcgcccggc tgctgaccga catcgtgcgg ctgctccccg atggcgacgt
     1681 gaacctggag accgcgggca accggatgac gatccgcgcg gcgcgcaaca catatgcgct
     1741 gacgacgatg ccggttgacg actttccgga ctggccgaag gtcgagccgc agctgacgat
     1801 cgtcctgcgc caggccgacc tcaagcgcgc gctgcacaat acgacgttcg cgatcccgac
     1861 gcgcgacccg cgcaaggtcc tgatgggggt gctgttcgac gtggccgagg gcaagctgac
     1921 gtgcgtcgcg accgacggcc gcaagctggg caagtcgatc atcgagccgg tcgagatcat
     1981 cggcggctcg tcgatccagg cgatcctgcc cgagcggatc ctgaccgaga tcgatcacgc
     2041 gatcggcgag gagggggaga ttcggattgc gttcgccgag cgccaggtcg ttttttcctt
     2101 gagcaatctg acctacatct ccaaccggat cgaggggacg tatccaaagt acgaggcggt
     2161 gatcccgcag acgttcaagc ggacgatcaa ggtgcagaag acgatcctgt ccgacgcgat
     2221 cagccgcgcg gcgatcctgg ccgagcgcaa gcaccactcg atcgtgctgc atttcaccaa
     2281 tggccagatt gagatcaacg cccgctcgtt cgaggacggt tcgtacgagg ggtcggtcga
     2341 aaccgattac gacggcgagc cgttccggat cgcgttcaac taccagtatc tgcaggagat
     2401 cttcaaagtt gcgcccgatg ccgtgatcga aatgaagatg aaggaggccg ccgcgccggt
     2461 cgtgttcgaa tgcgaaagcg atcccgattc gctctatctg atcatgccgg tgcggatgac
     2521 cgacatagac gacgtcccgc agccggcggc cgcgggcgct gttgaagatt gaggccggga
     2581 cgcgctgaat gaacgcaatc gggatcgtcc cgcccccgcg cgccggccag tttcctcccg
     2641 aggggggcgg cgacggccct tccgccgccg acccagccgc cgcaccgcgc cggttcgatc
     2701 cgtggccgtg ggtcatcctg ggcgttttcg ccgtcctatc gttcaccatg atcctggcga
     2761 tgtggtacgg ctaccgcatc ggccggttca cgcagaaggc ggaggcgctg atcgccgccg
     2821 agcgctacgc cgnnnnnnnn nnagtgttac ctggaaatgg gccagccgga ggaggcgctg
     2881 gcgcacctgg aactggtccg ccagtatgcg cccaacgagc cgctggatgt ggaatacggc
     2941 attgcgtggc ggctgcaggg caagcacgag gaagcggcgg aatccttccg caaagcgctg
     3001 gcgcgcaaac catccgacgc cgccgcgcat ttctatctgg gtttacaggc gcaggaggcc
     3061 ggccggatcg cggaagcggc gacgcatttc cagggggcgg cggccgatcc caagtgggac
     3121 cggcgcgccc tgccgcatcg ccagcaactg gccgacgccg tgctgggcga tgtggcgccc
     3181 aaactggagg cgcggccgcc ggcggacgcg aagccggagt gactgcgcgt cgcccgcgcg
     3241 accatcaatc gctcgtcgtt tcccgaatgc agatcggttc cgggcgccgc tgccaggggc
     3301 ttggatcatg ggaggcggcg acgcagggtt ccggcctgcg gccgtcaccc tgcgctttaa
     3361 accggtcgtc cgctctgcgg actgaaccga tcaacaagtc ggaagcgccg gcatggaacc
     3421 tgaaatcctg aagcagcttg cgatttgggg aatagcgagc actcaaaagc cgggtccgag
     3481 ccgatagatg tcgccgtcgc tgaccgagcc gttggtcggg tcatagctca ggtcgttgag
     3541 cgcgccctgc aacccgccgt agggctccag cggcccggac agattctggg gctttccttc
     3601 aaaaatatag acgacgccgc gctcgttgat gtcgccgagg cgggcgggga cgcccgggtc
     3661 catgaacgtg ctgagcgggt tgaggctggc cgtgccggcg cccagcttgc cctggacgtc
     3721 ctccagcagg tcggcatccg ggccgaagga ggtcaggatg tagccggtgc cgtgctcctt
     3781 atcggggcgg tagcagtagg tcatgaagcc ggcggcctgc tcggcgaagg ggtcggtggg
     3841 aaacgaggtg gcgtaggcga cgggggtggt cagggtgtgg aaatcgcggc cggcgatcag
     3901 gttgccgtcg cgggtgcaaa gcgtgtagta accggaggcg cggttgccga ggaagtcggc
     3961 gatttgctgg gggtagcggg cggggttgtc ggttccctcg gggtagtggt tgtgatcgac
     4021 gcgataggct tccagcccgg tggcgagggt gcgcagatcg ctgtggacgc gggagacctt
     4081 ggagcggatc tgcgcttcga ggaagttggg gacggcgatg gcggccagga tggcgatgat
     4141 ggcgacaacg atgagcagct cgatgagggt aaaggccgag ggggaggagg ccgcggggcg
     4201 ggataagcgc atgatgacaa ctgtctccta tggatttctc gcgttgacta tagtcaacga
     4261 ccggtgatgc taattatcgg gaggccggtt cgcaacaaaa aaagtgatcc atgtcgggtt
     4321 tttttttcgg tgccgcgcgc gttcgattca ggtcggggga ggcggggctg atatcgggcg
     4381 gat
//
LOCUS       DAOPCO010000330         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16659_length_4340_cov_3.855776, whole genome shotgun sequence.
ACCESSION   DAOPCO010000330 DAOPCO010000000
VERSION     DAOPCO010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16659_length_4340_cov_3.855776"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLS90_15760"
     CDS             <1..985
                     /locus_tag="PLS90_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPK03903.1"
                     /translation="RRMITRPHGIIYVTGPTRSGKSTTLYAALNEINNTDLKIMSIED
                     PIEYRINGVMQVQVNPSIELTFARCLRTFLRQDPDVIMVGETRDQETAQITIQTSLTG
                     HLVFSTLHTNDACSAVTRLLDMGIEPFLISSSLEGLLAQRLVRRLCPHCKQPAKADPA
                     QLLRVNVTNDDPSTFQLYKNRGCEKCRYTGYIGRTAIYEIAQMTETFRRMIVDRAPAN
                     ELKRQAIKDGMRPMRHDGWAKAKAGLTTIEEVLRVTMEEELEQFEEDNELKEALAANM
                     EGAEPVEEEGVETPPARIVEATGNSGTEPAQPSGEGDGREGSEAVRFTIER"
     gene            1112..2359
                     /locus_tag="PLS90_15765"
     CDS             1112..2359
                     /locus_tag="PLS90_15765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPK03904.1"
                     /translation="MPIFSYKAKDRRGEIVAGNLDADSRAAATARLQAMGLFPIAIQG
                     GEERSAAGPSRVKRALKVELRRGGRIRSEDLADFYRQMSDLIGAGVPLVKALGIVKNQ
                     TPNSALQGVLSQVSSDVQGGDTFAGALDKHPRTFTRLTVALVRAGETGGLLDETLNRI
                     ADYAESQDELRAKVKSALAYPMVMVIVGVIAVVVLLTFVMPKVLTIFDELDQTLPAIT
                     QVLIAITEFLPKYWLPIVGGAGVLYVLGRRYIKSPTGALRYHTTLLKVPKLGDLILKR
                     EVAAFARTLGSLLRNGVPILNALSIAAEVMTILPVRREIEKIPESITQGAGIAPTLRG
                     SALFPPVVVNMVAIGEETGHLPDVLLRVAESYETQVERSVKTLTSFIEPIIILVLGLV
                     VGFIVMAMLLPIFSIDPTQGTGV"
     gene            2362..2961
                     /locus_tag="PLS90_15770"
     CDS             2362..2961
                     /locus_tag="PLS90_15770"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HPK03905.1"
                     /translation="MERRRRGFTFFEIMLVIVIVGILTAVALPRMRGTYRRSQLTAAA
                     RELVGLMRYARNAAVLRELPVQIVFDLEEQRYQLIPLDENQERAEERTGRRRRRSEEG
                     ETRFTTDTDTGGVRRLTKARITALYTDAPPEEKTDLPRIIYYADGSATPATIVVQDGP
                     DHALHVVIYRTTGMAKVEPGVPEEEPETRTLFYGPGYGR"
     gene            2958..3416
                     /locus_tag="PLS90_15775"
     CDS             2958..3416
                     /locus_tag="PLS90_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK03906.1"
                     /translation="MSGARRGFVLLEVLLSLTILGMAVAAFMHSFTQSLRSARLMEIR
                     TQAMFFADQLMDEFEIFPPASGRRTEGGFGEDYAPYYYEVEMKVEEPRYRLRDEPEEV
                     ERFFATRVYTLEIYYRDESMAEPLLAARVSSAVVGFEKFSSQTKQSYAYF"
     gene            3403..>4340
                     /locus_tag="PLS90_15780"
     CDS             3403..>4340
                     /locus_tag="PLS90_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPK03907.1"
                     /translation="MRTFSRQPQGRFARGRGFTLLEILIASVIFGLTSVAIITVLRTG
                     VNAWRTGHRLSEVMQTARIAQDVVLRDLNNLMYLPEDQYNNVFKQQLQQLAQEVALDP
                     DYDYLRGDRERRRERERRRREREREREENRGEDRREQDPLSLDRIAPPIDLTFEGQDG
                     GDKDRLTFTRAYQPRWPGDPQTWGIRRVSFYVEDGTLYRKEEDAFSYSPGSIVNRRDL
                     LANPRVQSLYDSLRGNTRIDLDAFFGMSTTESSYDQLASMYYDPELEVLVPRMVEIAE
                     PLCEGVEVFNITYGYFRDEQWVEVGDWNSGATRYRDP"
BASE COUNT          785 a         1159 c         1714 g          682 t
ORIGIN      
        1 gcggcggatg atcacgcggc cgcacgggat catctacgtg acggggccga ccaggtcggg
       61 caaatcgacg acgctgtatg cggcgctcaa cgagatcaac aacaccgacc tgaagatcat
      121 gtcgatcgag gatccgatcg agtaccggat caacggcgtg atgcaggtgc aggtcaatcc
      181 ctcgatcgag ctgacgttcg cgcgttgcct gcgcacgttt ctgcgccagg accccgacgt
      241 gatcatggtg ggcgagacgc gcgaccagga gacggcgcag atcacgatcc agacgtcgct
      301 gaccggccac ctggtgttct cgacgctgca cacgaacgac gcctgctcgg cggtgacgcg
      361 cctgctcgac atggggatcg agccgtttct gatcagttcg tcgctggagg gtctgctggc
      421 gcagcggctg gtgcggcggc tgtgtccgca ctgcaagcag ccggcgaagg ccgatccggc
      481 gcaactgctg cgggtgaatg tgacgaacga cgatccgtcg acgttccagc tctacaagaa
      541 ccggggctgc gagaaatgcc ggtatacggg ttacatcggc cggacggcga tctatgagat
      601 cgcgcagatg accgagacgt tccggcggat gatcgtggac cgggcgccgg cgaatgaact
      661 gaagcggcag gcgatcaagg acgggatgcg tccgatgcgt cacgacggct gggcgaaagc
      721 gaaggcgggg ttgacgacaa tcgaggaagt gctgcgcgtc acgatggagg aagagttgga
      781 gcaattcgag gaggacaacg agctgaagga ggcgttggcg gcgaacatgg agggggcgga
      841 gccggtggaa gaagaagggg tggagacgcc gccggcgcgg atcgtggaag cgacggggaa
      901 ttcggggacg gagccggcgc agcccagcgg cgagggggat gggcgcgagg ggagcgaggc
      961 ggtgcgtttt acaatcgaac gctgagctgg cggtccgccc gcgagggggc ggcagcggcg
     1021 cagcgcgacg cggacaagcc ggggggcggc ggcgccggcc gccagcgacc gcgccggcca
     1081 atctcgaaag tcagcctgaa ttcacgcgca gatgccgata ttcagttata aagccaagga
     1141 tcgccgcggc gagatcgtcg ccgggaatct ggatgcggac agccgcgcgg cggcgacggc
     1201 gcgcttgcag gcgatggggc tgtttccgat cgcgatccag gggggagaag aacggagcgc
     1261 cgccgggccg tcgcgggtca agcgggcgct gaaggtcgaa ttgcggcgcg gcgggcggat
     1321 ccgcagcgag gacctggccg acttctaccg gcagatgtcg gacctgatcg gggcgggggt
     1381 gccgctggtc aaggcgctgg ggatcgtgaa gaaccagacg ccgaacagcg cgctgcaggg
     1441 ggttctgagc caggtcagtt ccgacgtgca ggggggcgat acgttcgccg gcgcgctgga
     1501 caagcacccg cggacgttca cgcggctgac ggtggcgctg gtgcgcgcgg gggagacggg
     1561 cggcctgctg gacgagacgc tcaaccggat cgcggactat gcggagagtc aggacgagtt
     1621 gcgggcgaag gtgaaatcgg cgctcgccta cccgatggtg atggtgatcg tgggcgtgat
     1681 cgcggtggtg gtgctgctga cgttcgtgat gccgaaggtg ctgacgattt tcgacgaact
     1741 ggatcagacg ctgccggcga tcacgcaggt gctgatcgcg atcacggagt ttctgccgaa
     1801 gtactggctg ccgatcgtgg ggggggcggg ggtgctgtat gtgctggggc ggcgctacat
     1861 caagagcccg acaggggcgc tgcggtatca cacgacgctg ctgaaagtgc ccaagctggg
     1921 cgatctgatc ctgaagcggg aggtggcggc gttcgcgcgg acgctcgggt cgctgctgcg
     1981 caacggggtg ccgattctga acgcgttgtc gatcgcggcg gaagtgatga cgatcctgcc
     2041 ggtgcggcgc gagatcgaga agatccccga gagcatcacg caaggggcgg ggatcgcgcc
     2101 gacgctgcgg ggcagcgcgc tgttcccgcc ggtcgtggtg aacatggtgg cgatcggcga
     2161 ggagacgggg catctgcccg acgtgctgct gcgggtggcc gaatcgtatg agacgcaggt
     2221 ggagcggtcg gtgaagacgc tgacgtcgtt catcgagccg atcatcatcc tggtgctggg
     2281 gctggtggtg gggttcatcg tgatggcgat gctgctgccg atcttctcga tcgacccgac
     2341 gcaggggacg ggcgtgtaac catggaacga cggcgccgcg gcttcacgtt tttcgagatc
     2401 atgctggtga tcgtgatcgt cggcatcctg acggcggtgg cgctgccgcg gatgaggggg
     2461 acgtaccggc ggagccagtt gacggcggcg gcgcgcgagc tggtggggct gatgcgctac
     2521 gcgcgcaacg cggcggtgct gcgcgagctg ccggtgcaga tcgtgttcga cctggaggag
     2581 cagcgctacc agctcatccc gctggacgag aaccaggaac gagccgagga acggacggga
     2641 cgccgccggc ggcgcagcga ggagggggag acgcgcttta cgaccgatac ggacacgggg
     2701 ggcgtccggc ggctgacgaa ggcgcggatc acggcgctgt acacggatgc gccgccggag
     2761 gaaaagaccg acctgccgcg gatcatctac tacgcggacg ggtcggcgac gccggcgacg
     2821 atcgtggtgc aggacgggcc ggatcacgcg ctgcacgtgg tgatataccg gacgacgggg
     2881 atggcgaagg tggagccggg ggtgccggag gaggaaccgg agacgcgaac gctgttttat
     2941 ggcccggggt atgggagatg agcggggcgc ggcgcggatt cgtactgctg gaggtgctgc
     3001 tgtcgctgac gatcctgggg atggcggtgg cggcgttcat gcattcgttc acgcaatcgc
     3061 tgcggtcggc gcggctgatg gagatccgga cgcaggcgat gtttttcgcc gatcaactga
     3121 tggatgaatt cgagattttt cccccggcga gcggccggcg gacggagggc gggttcggcg
     3181 aggactacgc gccgtattac tacgaggtgg agatgaaggt cgaggaaccg cgctaccggc
     3241 tgcgggatga accggaggag gtggaacggt tcttcgcgac gcgggtttac acgcttgaga
     3301 tctactaccg ggacgaatcg atggccgagc cattgctggc ggcgcgggtc agttcggcgg
     3361 tggtcggctt cgagaaattt tccagccaga cgaagcaatc ctatgcgtac ttttagccgt
     3421 cagccgcagg gccgcttcgc gcgggggcgc ggattcacgc tgctggagat tctgatcgcc
     3481 agcgtgatct tcggactgac gagcgtcgcg atcatcacgg tgctgcggac gggggtcaat
     3541 gcctggcgga cgggccaccg gctgtcggag gtgatgcaga cggcgcggat cgcccaggat
     3601 gtcgtgctgc gcgatctgaa caacctgatg tatctgcccg aagaccagta caacaacgtg
     3661 ttcaagcagc agctgcagca gcttgcgcag gaagtggcgc tggatccgga ctacgattac
     3721 ctgcgggggg acagggaacg gcggcgggag cgggagcggc ggcggcgcga gcgggaacgg
     3781 gagcgcgagg agaaccgggg cgaggatcgc cgggagcagg acccgctgtc gctggaccgg
     3841 atcgcgccgc cgatcgacct gacgttcgag ggacaggacg ggggcgacaa ggatcggctg
     3901 acgttcacgc gggcgtatca gccgcgctgg ccgggggacc cgcagacgtg ggggatccgg
     3961 cgcgtctcgt tttacgtcga ggatgggacg ctgtatcgga aagaggagga cgcgttttcg
     4021 tacagtccgg ggtcgatcgt caatcggcgg gacctgctgg ccaacccgcg ggtgcaaagc
     4081 ctgtacgaca gcctgcgggg gaacacgcgg atcgatctgg atgcgttttt cgggatgtcg
     4141 acgacggaat cgagttacga ccaactggcg agcatgtatt acgatccgga gctggaggtg
     4201 ctggtgccgc ggatggtgga gatcgccgag ccgctgtgcg aaggggtgga ggtcttcaat
     4261 atcacgtacg gctatttccg ggatgagcag tgggtggagg tgggggactg gaattcgggg
     4321 gcgacgcgct atcgcgatcc
//
LOCUS       DAOPCO010000331         4321 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_16750_length_4321_cov_5.115096, whole genome shotgun sequence.
ACCESSION   DAOPCO010000331 DAOPCO010000000
VERSION     DAOPCO010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16750_length_4321_cov_5.115096"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4261)
                     /locus_tag="PLS90_15785"
     CDS             complement(<1..4261)
                     /locus_tag="PLS90_15785"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPK03908.1"
                     /translation="MDRFKREASVQSPAAAGGRRYTAPQRWLYLLLFALTSTGWGAIF
                     FPTNQSELSLAIDQANLNAEDDVIDLQGLTITLTSPAAGENGLPPILFDGGHRVTIEG
                     SGGILERSANTEASFRLIYIDGGNLTINELTLRNGVAEFAGGAIVNHGTLSLNRCTLS
                     DNEAGSVGGAIYNGADLQISQSTISHNQAEWGGGIACSNEASGTFNMDQSTVSHNSAR
                     YDGGGILIDFPGLVGIISNTTISMNTAGTSGGGMYIGFFTSISMLHCTVTQNTADTSM
                     TNEGAGGGIYSEGLSIQATIVAGNHSYTEPFEIIPFEETLEIAPTGVTPTLYDSDVAG
                     IFFSGGNNIIGTKLGSSGFDGHGVTAQEQTPQVIEGDPDIELTQLNLTVDDILEPTLK
                     DNGGATESHNPLEGSIVVDLISPQSCIVQVDQRGTIRPQGDGCDAGSIEFSGPLAPAP
                     ALTATLDDELTSDTNHDGLLNPGETLRYTTVITNPHTGPTSTDTTFVLTLDPNTTLRL
                     GSIHTTPIAVPTSVTLFANTPTMITLTGSDADGDMLSFAITTPPSSGTLGAIVPLSPT
                     TAQVLYTPDSNFVGIDQFLFSVTDEDAAVDTAKVALEVISTPPNVPDAMLSAIGNTEL
                     VGVGGVATAHGAAFTTTSTNLLANVTDLESPPQILAVLAGSGNSLQGGDYEVYADGTF
                     TYVPPSGYNGSDSFNFTVSDGFVQTVATAHVDVAGMIWYVDNSVGSVGDGRSTNPYRA
                     LDNAGLASGPGDTVYVFYGDGSDRFQNVGFSMQTDQRLLGEGVDLVFGSQTLFKGDPG
                     RRPQISGVSHGVEAINDGNIEIAGLSLNVTAGLGIDITNTVAGASTIKITSNEIAAAF
                     DGIAITGAAGAIELFIDDNIVRSAATGIAINGLSIATVTIRSFANNGVDSAAFGGILI
                     SNVTFDADPGPDLVAVDGGVTRIGDPTITDFGLRLGGVFGALGFDSLSITNSGGEGLW
                     VRDGGGKPGNFALSIADGSISTTNGTAIDIDPVALDVTLSQLQSSGGNQGIRLATVTG
                     RVEIQSGHIEDKALPTIEIVRGDVEFYYHGTITNTSSYLLQVYQTTGGLVHLDGPTTL
                     SFTDSGNGIEIDGAAGDVFFSNGYFSGMRGIEFTGGPLRPATGNYQINNTTIRPTVPA
                     DPAHEPFVIHGGAGDDVLASIDLNNLHIENIVGNPLLIMGMGGGSVDLDEQSGISQLN
                     GQDGISCASNSGGVINLGGQIMLGTATHDAIHLVDNAGSTINFQPSELSITTTSGVGF
                     NAEQGGTVTVSGATNSISTATGSALRIEDTTIGDNGVTFKSISTNGAANAIILDNTGD
                     GPFTVTGDFGLAPTGAGGTIQNCTDDAVTLSRTGPVQLQQLTIDSNDGHGIYGEDVRG
                     FTVLACKVNDNGDAVAKTGSGIQLGDGVTTGVTGTVTIKESY"
BASE COUNT          739 a         1294 c         1447 g          841 t
ORIGIN      
        1 tgtagctctc cttgatcgtg acggtgccgg tcacgcccgt cgtgacgccg tcgcccaact
       61 gaatcccgct gccggtcttg gcgacggcat cgccgttatc attgaccttg caggccagca
      121 ccgtgaagcc gcggacatct tcgccgtaaa tcccatggcc gtcgttggaa tcgattgtca
      181 actgctgcag ctggaccggt ccggtccgac tcagtgtaac ggcgtcgtcg gtgcagttct
      241 ggatggttcc gcccgccccg gtgggggcca ggccaaagtc gccggtgacg gtaaatgggc
      301 cgtcgccggt gttgtcgagg atgatcgcgt tggcggcgcc gttggtggaa atcgacttga
      361 aggtgacccc gttgtcgccg atggtcgtgt cctcgatcct gagcgcgctc ccggtcgccg
      421 tgctgatcga gttcgttgcg ccgctcaccg tgacggttcc gccctgctcg gcgttgaagc
      481 cgacgccgga ggtggtcgtg atgctcaact cgctcggctg gaaattgatc gtcgacccgg
      541 cattgtcgac cagatggatg gcgtcgtggg tcgccgtgcc gagcatgatc tgaccgccca
      601 ggttgatgac gccgcctgaa ttgctcgcgc agctgatgcc gtcctgccca ttgagctgac
      661 tgatgcctga ttgctcgtcc aggtccaccg agccgccgcc catccccatg atcagcaagg
      721 ggttgccgac gatgttttcg atgtgaagat tattcaagtc gattgaggca aggacatcat
      781 cgccggcgcc gccatggatc acgaacggct cgtgggcggg gtcggccggc acggtcggac
      841 ggatggtcgt gttgttgatc tggtagttgc ccgtcgccgg acggagcggc ccccctgtga
      901 attcaatgcc gcgcatgccc gagaagtatc cattggagaa gaagacatcg cccgccgcgc
      961 cgtcgatctc gatcccgttg ccgctatcgg tgaatgagag cgtggtcgga ccgtcgaggt
     1021 gcaccaggcc gcccgtcgtc tggtagacct gcaacaggta gctgctcgta ttggtgatgg
     1081 ttccgtgata gtaaaactcg acgtctcccc ggacgatctc gatcgtcgga agggctttgt
     1141 cttcaatgtg gccggactgg atctcgactc ttccggtcac cgtggcaagc cggatgccct
     1201 ggttcccgcc ggaggactgg agctgggaca gggtcacatc gagcgctacc ggatcaatgt
     1261 cgatggccgt gccgttggtg gtgctgatcg atccgtcggc gatggacagc gcgaaattgc
     1321 cgggcttgcc gccgccgtcg cgcacccaga gcccctcccc gccgctgttg gtgatgctca
     1381 acgaatcgaa gcccagcgcg ccgaaaacgc cgcccagtcg cagaccgaaa tcggtgatcg
     1441 tcgggtcgcc gatccgggtg acgccgccgt ctacggcgac caggtccggg ccgggatcgg
     1501 cgtcgaaggt aacatttgaa atcaaaatgc cgccgaacgc ggcgctatcg accccgttgt
     1561 tggcgaaact gcggatggtt accgtcgcga tgctcaagcc gttgatagcg atcccggttg
     1621 ccgcggagcg gacgatgttg tcgtcgatga acagttcgat cgcgcccgcc gccccggtga
     1681 tcgcaatccc atcaaaggcg gcggcgatct cgttggaggt gatcttgatc gtggatgcgc
     1741 cggcaacggt gttggtaata tcgattccca agccagcggt aacgttgagc gagaggccgg
     1801 caatctcaat atttccatca ttgattgctt cgacgccatg gctcacgccg ctgatttgag
     1861 gccggcgccc ggggtccccc ttgaacagcg tttgcgaccc gaagaccagg tcgaccccct
     1921 cgcccagcag ccgctgatcg gtctgcatgc tgaacccgac gttctgaaaa cggtcgctgc
     1981 cgtcgccgta gaagacatag accgtgtcac cggggccgga agccaacccg gcgttgtcca
     2041 aagcgcggta gggattggtg gagcggccgt cgccgaccga accaacggag ttgtcgacat
     2101 accagatcat accggcgacg tcgacatggg ccgtcgcgac ggtctggacg aacccatcgc
     2161 tgaccgtgaa gttgaagctg tcggagccgt tgtaaccgga gggcggaaca taggtgaacg
     2221 tcccgtcggc gtacacttca tagtcgcccc cctggagcga gttgcccgag ccggcgagca
     2281 cggcgaggat ttgcggcggg ctctccagat cggtcacgtt ggccagcagg ttcgtgctgg
     2341 tggtggtgaa ggcggcgccg tgggcggtcg cgacgccgcc gacgccgacc agttcggtat
     2401 tgccgatggc cgacagcatc gcgtccggga cattcggggg cgtgctgata acctccagcg
     2461 cgaccttcgc cgtgtcgacg gcggcgtctt cgtcggtgac gctgaacagg aactggtcga
     2521 tgccgacgaa attggaatcc ggcgtgtaga gcacctgggc cgtcgtcggg gacaacggta
     2581 cgatcgcccc cagcgtgccc gaactcggcg gggtggtgat ggcgaagctc agcatgtcgc
     2641 catcggcgtc gcttcccgtc agcgtgatca tcgtcggcgt gttggcgaac agggtcaccg
     2701 aggtcggcac ggcgatcggc gtggtatgga tggaaccgag ccggagcgtc gtgttggggt
     2761 ccagcgtcag cacgaacgtg gtgtcggtgc tggtcggccc cgtgtgcggg ttggtgatga
     2821 cggtggtgta cctcagcgtc tcgccgggat tgagcaggcc gtcgtggttg gtgtcagatg
     2881 tgagctcgtc gtcaagcgtg gcggtcaggg cgggggcggg ggccagcggc ccggagaact
     2941 caatcgagcc ggcatcgcag ccgtcgcctt gcgggcggat cgtcccgcgc tgatcgacct
     3001 gcacgatgca ggattgcgga gagatcaggt caaccacgat cgagccctcc agcggattgt
     3061 gcgactcggt cgctccgccg ttatccttca gggtcggctc gaggatatcg tcgacggtca
     3121 gattgagctg cgtcagttcg atgtcggggt ccccctcgat cacctggggc gtctgctcct
     3181 gcgcggtcac gccgtgaccg tcgaagccgg aactgcccag cttcgtgcca atgatgttgt
     3241 tgccgccgct gaagaagatg ccggcgacat ccgagtcgta gagcgtcggc gtgacccccg
     3301 tgggggcgat ctcgagagtc tcctcgaacg gaataatctc gaacggttcg gtataggaat
     3361 ggttgccggc gacgatcgtc gcctggatcg agagcccttc ggaataaatc ccgccgccgg
     3421 ccccctcgtt cgtcatcgag gtgtcggccg tattctgcgt gacggtgcag tgcaacatgg
     3481 agatcgacgt gaagaagccg atatacatcc cgccgcccga ggtgccggcc gtgttcatgc
     3541 tgatcgtagt gttggagatg atgccgacca ggcccggaaa gtcgatcaaa atgccgccgc
     3601 catcgtagcg cgcactgttg tgggaaaccg tcgactgatc catgttgaat gtgccgctcg
     3661 cttcgttgga gcaggcgatg ccgccgcccc actcggcctg attgtggctg atggtggact
     3721 gggaaatctg cagatcggcg ccgttataga tcgcgccgcc gacgctcccc gcctcgttgt
     3781 cggacagcgt gcagcggttc aagctgaggg ttccgtgatt cacgatggcg ccgccggcaa
     3841 actccgcgac gccgttgcgc agggtcagct cattgatcgt caagttgccg ccgtcgatgt
     3901 agatcagccg gaaactcgcc tcggtgttgg cactacgttc caggatgccg ccgctccctt
     3961 cgatcgtcac gcgatgaccg ccgtcgaaca ggatcggcgg caagccgttt tcccccgccg
     4021 cgggcgaggt aagcgtaatg gtcagtccct gaaggtcgat cacgtcgtct tcagcattga
     4081 gattggcttg atcgatggcc agggacagtt cggattggtt ggtcgggaag aagatggcgc
     4141 cccatccggt ggaggtcagc gcgaagagca gcagatagag ccaacgctgc ggcgcggtgt
     4201 accggcggcc accggcggcc gcgggagatt gaaccgatgc ttcgcgcttg aagcgatcca
     4261 tgatgggcga cctttccagg tggaccacga acaacctgca ccaacccggc ggcaccggga
     4321 t
//
LOCUS       DAOPCO010000332         4263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_17026_length_4263_cov_5.233603, whole genome shotgun sequence.
ACCESSION   DAOPCO010000332 DAOPCO010000000
VERSION     DAOPCO010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17026_length_4263_cov_5.233603"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLS90_15790"
     CDS             complement(<1..371)
                     /locus_tag="PLS90_15790"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK03909.1"
                     /translation="MRRTALVTGITGQDGAYLAKFLLEKDYKVYGLIRRSVRHAFENL
                     EYLGVDREIEYLNGDMTDESSLIKAVHQTRPDEVYNLAAQSFVGASWEQPHLTTQVNS
                     IGVLNLLEAVKLHAPRARFYQA"
     gene            complement(468..1829)
                     /locus_tag="PLS90_15795"
     CDS             complement(468..1829)
                     /locus_tag="PLS90_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK03910.1"
                     /translation="MSAELPRTDDGRETRLNRRELLKAGGLLAAAVGFPTIVPSSVFG
                     QHAPSNRITMASIGPGRMGTWDVIEVLAFADVQFVAACDVDRKRLENARNTIESHYAR
                     RSGSGCYKGCFATTDFREVIARDDLDAVMITTPDHWHALPALAAARAGKDLFVQKPLT
                     LTIPEGRALSDAARRRGVVVQVGSQQRSARDFRRACELVRNGRIGRLQTIRIELPGDQ
                     GGPLAAPTPPPAHLDYDLWLGPAPWRDYVEQRVMPREGYDRPGWLRAEDYTNGMITNW
                     GVHHVDIAHWAMGCELGGPAEVEAKAERLSGGVWDVHGDFEVTYRYADGVTVIVAGNE
                     KSSEGVRFEGSEGWVRVTRFGMHAEPASLLTSRIGPEELHLYESNHHKRNWVDCIRSR
                     RETAAPVEVGHRANSACILGWIAMKLNRRLQWDAERERFLGDEEANRLLHRPMREPWH
                     GRA"
     gene            1984..2394
                     /locus_tag="PLS90_15800"
     CDS             1984..2394
                     /locus_tag="PLS90_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03911.1"
                     /translation="MSSETPTLSTRPVAPASIEDYFAGRYRLVLILVLLHQAWLFGLV
                     RLGRSGLLTEPRILLPSLAGPALLFLLLATANTLWERWSPRGGPDQPGGDPLGAWRRD
                     LPLHVVFFDAIALCFVSLLLGILGFMVLGLVLFD"
     gene            complement(2411..2701)
                     /locus_tag="PLS90_15805"
     CDS             complement(2411..2701)
                     /locus_tag="PLS90_15805"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HPK03912.1"
                     /translation="MGLYVMLGEDSPEGTARRPDVRPAHLAYWGPMSAQGRVRYAGPL
                     LDAHEKPVGSVIIFEAEDLAAARTLAQGDPYVREGVFGILRCHASKQVLPEG"
     gene            3031..3894
                     /gene="lpxI"
                     /locus_tag="PLS90_15810"
     CDS             3031..3894
                     /gene="lpxI"
                     /locus_tag="PLS90_15810"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HPK03913.1"
                     /translation="MSATADLHSLDYATPLDPLTLRHIGLIAGEGRFPILLAQAARDR
                     GISVTAVGINGITSPELADYVDTMRWVEFGQFNRLIQICHEHNIRQAIMAGRVKHRSI
                     FQISKMDGRGMKLLARSVSRKADDLLGAVTREFAIENIEMLDSTLLLRQCMPPSGLLT
                     PNCPPTQEILDDIEFGKPLANAIAGHDIGQTIVVKQGAVIAVEAMEGTNQTILRAGEI
                     AGEGCVVVKVAKPRQDRRFDVPVLGLTTVKKLIQARGRAIAFPGSQALFFEVEEAVAL
                     AESRGITLLAW"
     gene            3927..>4263
                     /locus_tag="PLS90_15815"
     CDS             3927..>4263
                     /locus_tag="PLS90_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HPK03914.1"
                     /translation="MTLASLHARVEQALRRREAELIELHRALVRLPTVNHGDGRSADE
                     TAAARLARDFLARAGIQAEILEPAPGRGNLLASSGAADGPALLLMSHSDVVPAGDEQA
                     WIHPPFGADL"
BASE COUNT          669 a         1599 c         1239 g          756 t
ORIGIN      
        1 gcctggtaaa acctcgcgcg cggcgcgtgg agcttcaccg cttccagcag gttcagcacg
       61 ccgattgagt tgacctgcgt cgtcaaatgg ggctgttccc acgacgcgcc gacaaacgac
      121 tgcgccgcca gattgtagac ttcatcgggc cgggtctggt gcaccgcctt gatcagcgac
      181 gactcatccg tcatgtcgcc gttcaggtat tcgatttcgc ggtccacacc cagatactcc
      241 aggttttcga acgcatgccg cacgctgcgg cggatcaggc cgtaaacctt gtagtccttc
      301 tccaacagaa atttggccag atacgctccg tcctgacccg tgatgcccgt caccagcgcc
      361 gtgcgccgca tcttgctacc atcctttcat ccgttgttgg ggggcggcgg ggcgcacccc
      421 gctcaagtct ggcccatgcg agcggaaatt ctccagcaag aaccgcatca cgcccgccca
      481 tgccatggct cgcgcatcgg ccggtgcagc agccggttcg cttcctcatc gccgagaaac
      541 cgttcccgct cggcgtccca ctggagccgg cggttcagct tcatcgcgat ccagccgagg
      601 atgcacgccg agttcgcgcg gtgtcccact tccaccggcg ccgccgtctc ccggcgcgaa
      661 cggatgcagt ccacccagtt ccgcttgtgg tggttcgatt catacagatg cagttcctcc
      721 ggcccgatcc ggctcgtcag cagcgacgcc ggctcggcgt gcatcccgaa acgcgtcacg
      781 cgcacccagc cttcgctgcc ctcgaagcgc actccctcgc tgctcttctc gtttcccgcc
      841 acgatcaccg tgacgccgtc ggcgtagcga taggtgacct caaaatcgcc gtgaacgtcc
      901 cagacaccgc cgctcagtcg ctcggctttg gcctcgacct ccgccgggcc gcccagttcg
      961 caccccatcg cccagtgggc gatatcgaca tgatgcactc cccagttggt gatcatcccg
     1021 ttggtgtagt cttcggcgcg cagccagccc gggcggtcgt agccctcgcg cggcatcacc
     1081 cgctgctcca cgtagtcgcg ccagggggcc ggcccgagcc acaggtcgta atcgaggtgg
     1141 gccggcggcg gcgtcggcgc ggcgagcgga ccgccctggt cgcccggcag ttcgatccgg
     1201 atggtctgca accggccgat ccggccgttg cgcaccagtt cgcacgcacg gcggaaatcg
     1261 cgcgccgagc gctgctggct gcccacctgc accaccactc cccgccggcg cgccgcgtcg
     1321 ctcagcgccc gtccctcggg gatcgtcagc gtcagcggct tctgcacgaa caggtccttg
     1381 cccgcccgcg ccgccgcgag cgccggaagc gcatgccagt ggtccggcgt cgtgatcatc
     1441 accgcgtcaa ggtcgtcgcg cgcgatgacc tcgcgaaaat ccgtcgtggc gaagcaaccc
     1501 ttgtaacacc ccgaaccgga gcgccgggcg taatgactct cgatcgtgtt ccgcgcattc
     1561 tccagccgct tgcggtccac gtcgcacgcc gccacgaact gcacgtccgc aaacgccagc
     1621 acctcaatca cgtcccaggt gcccatccgg ccggggccga tcgacgccat cgtgatgcgg
     1681 ttgctcggcg cgtgctgtcc gaacaccgac gacggcacga tcgtcggaaa tccgaccgcc
     1741 gccgccagca gcccgccggc cttgagcagt tcgcgacggt tcaggcgggt ttcccgtcca
     1801 tcgtcggtcc gtggcagctc cgcgctcaag atgtcaatgc ttcctccggg gagttctctt
     1861 tcccgcatcg gggaggatgt ggggggcaat gcactcgatg gccgcatcag gttttatctg
     1921 acaagcccgc gaacctccgt tacaatcgac tcctaccccg tctcccaatc agggccgccc
     1981 ggcatgtcgt ccgagactcc caccctttcg acccgccccg tcgccccggc gtcgatcgaa
     2041 gactatttcg ccggccgcta tcgcctggtg ctcatcctcg ttctgctgca tcaggcctgg
     2101 ttgttcggcc tcgtccggct cggccggagc ggcctgctca ccgaaccgcg gatcctgctg
     2161 ccctcgctgg cggggcccgc gctgctgttc cttctgctgg ccacggccaa cacgctgtgg
     2221 gagcgctggt cccctcgcgg cgggcccgat caacccggcg gcgatccact cggcgcctgg
     2281 cgtcgcgacc tgccgctgca tgtcgttttc ttcgacgcca tcgcgctctg tttcgtcagc
     2341 ctcctgctcg gcatcctcgg cttcatggtc cttggtctgg ttttgtttga ttaacttccc
     2401 aaaacacacc tcagccctcg ggcaggacct gcttgctcgc gtgacagcgc aggatgccga
     2461 acacgccttc gcgcacgtag gggtccccct gcgcaagcgt gcgcgccgcc gccagatcct
     2521 ccgcctcgaa gatgatcacc gagcccaccg gcttctcatg ggcatccagc agcggcccgg
     2581 cataacgcac ccgcccctgc gcgctcatcg ggccccagta ggccagatgc gccggccgga
     2641 cgtccgggcg tcgggccgtc ccctccgggc tgtcctcgcc cagcatcaca tagagaccca
     2701 tcgcgactcg tcctcctgca agggattgaa acgagccatc gtgcccatga ccagcctgaa
     2761 atttcaaccc gggaacttcg gccgacagtc tttgtcggag ggcagcggcg gggtcatccc
     2821 ggggtctcca gatcgacaag cgataaggct tgtcatggga tctacggatt gaccccacgc
     2881 cacctttctg atagaaggag agggttttga cgggctgccg aatcgggcgt tttgccctcc
     2941 cccgttgtta cttgcctcgc ctcccgcagt accacccttg ccccgtcaaa ccggcgaggg
     3001 ggacgatgta caagcgaaag cccttgtccc atgagcgcca ccgccgacct tcattcgctt
     3061 gattacgcca caccgcttga ccctctcacc ctgcgacaca tcggactgat tgccggcgag
     3121 ggacggttcc cgatcctgct ggcccaggcc gcccgcgatc gcggcatctc ggtcaccgcc
     3181 gtcggcatca acggcatcac ctcccccgag ctggccgact acgtcgatac gatgcgctgg
     3241 gtcgagttcg gccagttcaa ccgcctgatc cagatttgtc acgaacacaa catccgccag
     3301 gccatcatgg ccggacgggt caagcaccgg tcgatcttcc agatctccaa gatggacggc
     3361 cgcggcatga agctgctcgc ccgctcggtc agccgcaagg ccgatgatct gctgggcgcc
     3421 gtcacgcgcg agttcgccat cgaaaacatc gaaatgctcg attcgacgct cctcttgcgt
     3481 cagtgcatgc cccccagcgg cctgctcacc cccaactgtc cacccacgca ggagatcctc
     3541 gacgacatcg aattcggcaa gccgctggcc aacgccatcg ccggccacga tatcggccag
     3601 acgatcgtcg tcaagcaggg cgccgtcatc gccgtcgagg cgatggaagg gaccaatcag
     3661 accatcctgc gcgcgggcga aatcgccggc gaaggctgcg tcgtcgtcaa ggtcgccaag
     3721 ccgcgccagg accgccgttt cgatgtcccc gtgctgggct tgaccacggt caagaaactg
     3781 atccaggctc gcggccgggc catcgccttc ccgggcagcc aagccctctt cttcgaggtc
     3841 gaggaggccg tcgccctcgc cgaatcccgc ggcatcacgc tgctggcctg gtaatccgcc
     3901 gttcctcccc acgaagcccg ccgctgatga cactcgcttc gcttcacgcg cgcgttgagc
     3961 aggccctgcg ccggcgcgag gccgaactga tcgaactgca ccgcgccctg gtccgcctcc
     4021 ccaccgtcaa ccacggcgac ggccgctcgg ccgatgaaac cgccgccgcc cggcttgcgc
     4081 gcgacttcct ggcccgagcc ggcatccagg ctgaaatcct cgagcccgcc cccggccgcg
     4141 gcaacctgct ggcatcctcc ggcgccgccg acggccccgc tctgctgctg atgagtcaca
     4201 gcgacgttgt tccggccggc gatgagcagg cctggatcca ccccccgttc ggcgcggatc
     4261 tcg
//
LOCUS       DAOPCO010000333         4224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_17218_length_4224_cov_5.020149, whole genome shotgun sequence.
ACCESSION   DAOPCO010000333 DAOPCO010000000
VERSION     DAOPCO010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17218_length_4224_cov_5.020149"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLS90_15820"
     CDS             complement(<1..376)
                     /locus_tag="PLS90_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03915.1"
                     /translation="MCATRTLRAAAALLLLAIQAACAVHHGGRQATETTPRYRGLMPA
                     FAFEPGKPKRENAEYKRQGKPDLAWMWFYNHRVNENWVVPIAYREEALEHTKRYNQKR
                     PKDVAAAWSYVGPGGQVDAPSNG"
     gene            complement(624..1178)
                     /gene="rnhA"
                     /locus_tag="PLS90_15825"
     CDS             complement(624..1178)
                     /gene="rnhA"
                     /locus_tag="PLS90_15825"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPK03916.1"
                     /translation="MNSRAAAKPTKRATGSEVLIYCDGACSPNPGLGGWGVVLLAPEK
                     GLRKELSGAEAESTNNRMELTAAIEGLRALKRPSRVRVITDSEYLQKAFTQGWLERWR
                     RNGWRTADKKPVKNIELWQALMQAMAEHDVEWSWTRGHGQDEENNRCDKLAVAARQRL
                     KRELEAEGRPVGGGGGGESIGGGE"
     gene            1430..2503
                     /gene="lpxD"
                     /locus_tag="PLS90_15830"
     CDS             1430..2503
                     /gene="lpxD"
                     /locus_tag="PLS90_15830"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPK03917.1"
                     /translation="MSTALYRPPAAVQPTPQQVADWVAGELRDGASAPARLAGLATLD
                     NARPDEIAFVANDKAATLADQSQAGLLLLAEPHDRPGRPRVVVKSVWRAVIDLMDRLY
                     PPRSAAGGIHPTAVVAPTAKLGAGVAIGPYCVVEDEAELGDGVELGPHCIVSAGCRIG
                     TGCRLVARVTLVGVVFLGRRVTVHPGAVLGSDGFKYEVVDGAPRKVPQVGAVIIDDDA
                     EIGANTTIDRAFLHETRVGRGVKIDNLVQIAHNVSVGDCSLLAALVGIAGSTRLGDGC
                     VVGGHVGIADGLNIAPRTTVGGKTGLTFSVDEPGQTLFGYPARPIKQFWRIHSVQARL
                     PEMARRLAQLEAKLQSLEAARQE"
     gene            complement(2590..2853)
                     /locus_tag="PLS90_15835"
     CDS             complement(2590..2853)
                     /locus_tag="PLS90_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03918.1"
                     /translation="MKRYLLPCVLAAVVLACSPTLVSAHEYDRDDSEHPLRLVGYALH
                     PIGIAAEYLILRPIHWVVSQRNLDIIFGHEVNEDPDYNYFEWE"
     assembly_gap    3324..3423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3515..3931)
                     /locus_tag="PLS90_15840"
     CDS             complement(3515..3931)
                     /locus_tag="PLS90_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPK03919.1"
                     /translation="MHLIEFELREIQMKEEGLGSRQIIVLGEKDGEREFPIIIGFAEA
                     LALDLDLHGYVYPRPMTHDLIFNVMEGLKAELKHVVVDDLRDGTFFGKLVVRTADDTE
                     EWIDSRPSDAIVLATKKKVPIYVNEQILERILNENE"
BASE COUNT          621 a         1563 c         1151 g          789 t
ORIGIN      
        1 ggccgttcga cggcgcatcg acctgcccgc ccgggccgac ataggaccac gccgccgcca
       61 catccttcgg ccgcttctgg ttgtatcgct tcgtgtgttc cagcgcctcc tcgcggtagg
      121 cgatcggaac cacccagttc tcgttgaccc gatgattgta aaaccacatc cacgccaggt
      181 ctggcttgcc ctgacgcttg tattcggcgt tctcgcgttt gggtttgccc ggctcaaacg
      241 cgaaggcggg catcagcccg cggtagcgcg gcgtcgtttc ggttgcctga cggccgccgt
      301 ggtgaacggc gcaggcggcc tggatggcga gcagcagcag cgccgccgcc gctcggagcg
      361 ttcttgtggc gcacatggcg tgcagtctcc ccggaggcgg gtcgcggcgc gccctctccc
      421 ggcaatttcc acttcttatt gacgccccaa cggcggatga gcgtaggtaa ttctaaatgg
      481 tgagtcggtc ggaccgcaat cgaacaattt taacgttatg cgaaaaaacg accgccggcc
      541 ggcgcactga acatcactcc ggtcatcccg gtttccaccc atccccacgc cggatctcac
      601 ttcaagccgt cgcccgcacg ggcttattcc ccaccgccaa tgctttctcc gccgcccccg
      661 cctcccactg gccgcccttc cgcctccagc tcccttttca gtcgctgccg ggccgcgacg
      721 gccagtttgt cgcagcggtt gttttcctcg tcctgcccgt ggccccgcgt ccagctccac
      781 tcgacgtcgt gctccgccat cgcctgcatc agcgcctgcc acagttcgat gttcttgacc
      841 ggcttcttgt cggccgtgcg ccagccgttg cgccgccagc gttccagcca cccctgcgtg
      901 aacgccttct gcagatactc gctgtcggtg atcacccgca cgcgggacgg gcgcttcagc
      961 gcgcgcaacc cctcgatcgc cgccgtcagt tccatccggt tgttcgtcga ttccgcttcg
     1021 gcgcccgaca gttccttgcg cagccccttc tccggcgcca acagcaccac gccccaccct
     1081 cccaggcccg gattcggcga gcaggcccca tcgcagtaga tcagcacttc ggatccggtg
     1141 gcgcgtttcg tcggcttggc agcggctctt gaattcatcg cgtccatggg accatgcttc
     1201 cggccccttg tgaagcataa ccgtgtccgt aaatttccgt ctcgcgtctc tcgtcccccg
     1261 tcctcgtctc tcgaatcacc ccgatccggt ccccatcgcc gaaattgcgt tctcatcccg
     1321 cggggctttg actacgatgg ttccccgtgg cacgctgcgg cgcacccgtg cccgcgcggg
     1381 tgcagcgcgc gcgccccccg cgctcgatct ccgaagggaa tcctgctcca tgtcaaccgc
     1441 tctctaccgg ccccccgccg ctgttcagcc aacgccccag caggtcgccg actgggtcgc
     1501 gggagaatta cgcgacgggg cgtcggcccc cgcgcggctg gccgggctgg cgacgctcga
     1561 caacgcgcgc cccgacgaaa tcgccttcgt cgccaacgac aaggcggcga cgctggccga
     1621 ccagtcccaa gccggcctcc ttctgctcgc cgagccgcac gaccggccgg gacgcccccg
     1681 cgtcgtcgtc aagtcggtct ggcgggccgt cattgacctg atggaccggc tctacccgcc
     1741 gcgttcggcc gccggcggca tccatcccac ggccgttgtc gccccgacgg ccaaactcgg
     1801 cgccggcgtc gccatcggac cctattgcgt cgttgaggat gaggccgaac tgggcgacgg
     1861 cgtggaactg gggccgcact gcatcgtctc ggccgggtgc cggatcggca cgggctgccg
     1921 cctggtcgcc cgcgtcacgc tggtcggcgt ggtcttcctc ggccggcgcg tcaccgtcca
     1981 tccgggcgcc gtcctgggca gcgatgggtt caaatacgaa gtcgtcgacg gcgcgccgcg
     2041 caaggtgccg caggtcggcg ccgtcatcat cgacgacgac gccgagatcg gcgccaacac
     2101 gacgatcgac cgcgccttcc tgcacgaaac gcgcgtcggc cgcggcgtca aaatcgataa
     2161 cctggtccag atcgcgcaca acgtctcggt gggcgactgc agcctgctgg ccgcgctggt
     2221 cggcatcgcc ggctccaccc ggctcggcga cggctgcgtc gtcggcggcc acgtcggcat
     2281 cgccgacggc ttgaacatcg ccccgcgcac gaccgtcggc ggcaagaccg gcctgacctt
     2341 cagcgtcgac gagccgggcc agaccctctt cggctacccg gcccgtccga tcaagcaatt
     2401 ctggcggatt cacagcgtgc aggcgcggct gcccgaaatg gcgcgccgcc tcgcccaact
     2461 ggaggccaag ctccagtcgc tcgaggcggc gcggcaagaa taaggtctgc ctgatccgga
     2521 ccccggccgt tcaagccttc cggcgcggcc ggtccgtcac atgggtgcca tcgggcttcg
     2581 gcccggcttt cattcccatt cgaagtaatt gtagtcgggg tcctcgttga cttcgtggcc
     2641 gaagatgata tcgaggttcc gctgcgagac cacccagtgg atcgggcgca ggatcagata
     2701 ctcggccgcg atgccgatcg gatgcagtgc atagcccaca agacgcaggg gatgctccga
     2761 gtcgtcgcga tcgtattcat gcgccgacac cagcgtgggg gagcaggcca gcacgacggc
     2821 cgccagaacg cagggcagga gataacgctt catcagtcgg ggctccttcg ggaagttctt
     2881 gtgctggggc ggacggcgca agcccgcggg ccggtgacac tgcccccgcc tcgtttgtct
     2941 catcggcctc ccaacccctc accttaagct ttttcccaaa attcgtccct cgtcctcgtc
     3001 cccactcaac aatccgtcct tcgtcctcgt ccccatgatc gcttccgaag gctcgcccca
     3061 ttttgtccct tcgttgtaat ccgccggatc ccagccccaa cctccgtgct gcgctcctcc
     3121 gcgtctcggc gtctcggcgt gagagcttca cccccaagga cttccccaaa attcggctct
     3181 cggctcgcgt ccctcgtcct cggctctcga aaaacctcgg cgcgtcttta cattgtaaac
     3241 cgccggatcc cagccccaac ctccgtgcct gcgctcctcc gcgtctcggc gtctcggcgt
     3301 gagagcttca cccccaagga cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnggctctc ggctcgcgtc cctcgtcctc ggctctcgaa aaacctcggc gcgtctttgc
     3481 atggccaatg cccttcctcg cacccccccc cgcctcactc attttcattc aggatccgct
     3541 ccaggatctg ttcattgaca taaatcggca ccttcttctt cgtcgccagc acgatcgcat
     3601 cggacggtcg cgagtcgatc cactcctccg tgtcgtcggc cgtgcgcacc accagcttgc
     3661 cgaaaaacgt cccgtcgcgc aggtcgtcca cgacgacatg cttgagctcg gccttcagcc
     3721 cttccatcac attgaagatc aggtcgtgcg tcatcggccg gggataaacg tagccgtgca
     3781 ggtccaggtc cagcgccagc gcttcggcga acccgatgat gattgggaac tcgcgctcgc
     3841 catccttttc ccccagcacg atgatctggc ggctgcccag accctcttcc ttcatctgga
     3901 tctcgcgcag ttcaaattcg atcagatgca tcccgtgatc ttccatggcc gcccggcccc
     3961 cgatccgtaa acagtcctca cccggaaccc gagtctaccg gtcggtcgcg ccgcctgtca
     4021 aacaaccctc acccccaacg gccctcccca agattcggct ctcggctccc gtccctcgtc
     4081 ctcggctctg aatccccccg attcacaccg tccaacctcc ctccatcccc ccaccccagt
     4141 ttttctggca ttccaccgcc ccccgagtga aaatgtttgc tactctcaag tgcaatggat
     4201 tgaacgtctg ccccccgatg aaac
//
LOCUS       DAOPCO010000334         4145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_17621_length_4145_cov_4.972127, whole genome shotgun sequence.
ACCESSION   DAOPCO010000334 DAOPCO010000000
VERSION     DAOPCO010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_4145_cov_4.972127"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..3183)
                     /locus_tag="PLS90_15845"
     CDS             complement(124..3183)
                     /locus_tag="PLS90_15845"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPK03920.1"
                     /translation="MHVVAPIVARALRRAALAAGLVGALVRPLAAPPGAGPIDWERPA
                     PAAAKAAVEAIKLTPEEEERAQRLHAESLVIVAHSHDFLAADFADAHTGGITAVVLKL
                     ITDSIDWNRDTRTRYPVPGLEGWTERFLGYLGDIRQIADNPENKVRVIETVDDIFAAK
                     AAGEVGAIIGSEGAMHLEGELANVERFHSLGWRETQLRWAANQLLDGSSLSEFGRQVI
                     AECNRLGVLIDISHLYGSEVPAVMANTEDPVVRSHDAPAHMGGEASDALIQLVANSGG
                     GNGVFCVHFGYLQPLTKVLDAIDYIVNLVGVDHVGIGADWYPETGTTFALDAEEMLEL
                     TRGLVERGYSDEEIEKILGLNLVRLYRIVWGEETEEERAERLHNSSIIILAHDHTFTA
                     GDFDAAREAGLTAKVLKLTTDGIDWENGQRYEVTSYEGWRERHLGYLEGVQALADNPE
                     ANVTIIRTVEDLYQAKVEGKIGAIIGSEGVRYLEGELEALEEFHALGTREIQLWWPAG
                     NQAIVNGQLTAFGRALIAECNRLGIVIDVSHMGGLALDVIALSEDPVIVSHDTPSTIG
                     GEASDALIQAVAASGGGHGVFAIHFINGYVTPPTVARVAYAIDYVRDLVGIEHVALGG
                     DYFPEDGVIWAIPVSELKELTLELVRRGYSDTEIRQVLGLNLIRLYERVWNKTAGGEA
                     ADEQPIVAGIAGQELLVYEVREGGIDPAQPAASVTLASQAIDVAVTQLAPGGEPEIVV
                     LRQGTPDQLSVYRRAGGELELIAEGSVLASDGIAVGAAAPRQGTATPAIGVLIDNGTA
                     ADTFSIFELNGDAIERSEGPRVFAQVGQWDYRGEYSGFALGQIVHTGGAPPLGGDPNK
                     LNAAFGTIWPDGRNIIHYAEVGSSHLAPVYLTAGWGGAASVDFGLFFKVGAPGDHIVG
                     GGGHNLANRVAWWEVPQGVGGAWEGARDVGAAVVDVAAVQLDGDTEDEIVYLTAGTVG
                     WIDPGSSGAGAGGTAANLPGLVRIDGTRVGEGAPIIRTPAGHWTIWR"
     gene            3719..>4145
                     /locus_tag="PLS90_15850"
     CDS             3719..>4145
                     /locus_tag="PLS90_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03921.1"
                     /translation="MRLANFLLSLAALALLFLASPPAGAQPILIDSNANAVAEDLALG
                     NFGQDAHAGVVAAWRDSASEDLAIDFYRYEELPSSNWAAERIAQVSDVATSPTDSPAP
                     AVRIVLGDYSRDGKTDLTVMYNSNLTTTSLKHYQRTSPGF"
BASE COUNT          646 a         1680 c         1098 g          721 t
ORIGIN      
        1 cccgcgagtc agtcgcatcc gcccgcattc tccgcgctta tcgatcctta aatctcggtc
       61 aacccaaccc ctgaaagggt ggccggagat gctggctcct tccgccagcc ggccgccttg
      121 ggatcagcgc cagatcgtcc aatgacccgc cggcgtgcgg ataatcggcg ccccctcccc
      181 cacgcgcgtc ccatcgatcc ggaccagccc cggcaggttc gccgccgttc cgcccgcgcc
      241 cgcgccgctg cttcccgggt cgatccaacc gacggttccc gccgtcagat acacgatctc
      301 gtcttccgtg tctccatcca actgaaccgc cgccacgtcc acgaccgccg cgccgacgtc
      361 gcgcgcccct tcccacgcgc cccccacgcc ctgcggaact tcccaccagg ccaccctgtt
      421 ggcgagattg tggccgcctc ccccaacgat gtgatcgccg ggcgcgccga ccttgaagaa
      481 caaaccgaaa tccacgcttg ccgccccgcc ccaccccgcc gtcagataca ccggcgccag
      541 gtggctcgac cccacctccg cgtagtggat gatgttgcgc ccgtcgggcc agatcgttcc
      601 gaaggccgca ttcagtttgt tggggtcgcc ccccagcggc ggcgcgcccc ccgtatgcac
      661 gatctgaccc agcgcaaacc cgctgtattc cccccggtag tcccactgac ccacctgcgc
      721 gaacacccgc ggcccctcgc tgcgctcaat cgcgtcgccg ttcagctcga aaatggaaaa
      781 cgtgtccgcc gccgtgccgt tgtcgatcag caccccgatc gccggcgtcg ccgtgccctg
      841 ccgcggcgcc gccgccccca ccgcgatccc gtccgacgcc agcaccgacc cttccgcgat
      901 cagctccagc tctccgcccg cccgccggta gaccgacaac tgatccggcg tcccctgccg
      961 cagcacgacg atctccggct cgccccccgg ggccaactgc gtcaccgcca cgtcgatcgc
     1021 ctggctcgcc agcgtcaccg aagccgccgg ttgcgccgga tcgatccccc cctcgcgcac
     1081 ttcatacacc agcaactcct gccccgcgat ccccgccacg atcggctgct cgtcggcggc
     1141 ctcgcccccc gccgtcttgt tccacacccg ctcatacagc cggatcaggt tcaaccccag
     1201 cacctgccgg atctccgtgt cgctgtatcc gcgccgcacc agttccagcg tcagctcttt
     1261 caactccgac accgggatcg cccagatcac gccatcctcc ggaaagtagt cgccccccag
     1321 cgccacgtgc tcgatgccga ccaggtcgcg cacataatcg attgcatagg ccacgcgcgc
     1381 caccgtcggc ggcgtcacat agccgttgat aaagtggatc gcgaacacgc catgcccgcc
     1441 cccgcttgcc gccaccgcct ggatcagcgc gtcgctcgcc tcaccgccga tcgtcgacgg
     1501 cgtgtcgtgc gacacgatca ccgggtcctc cgagagggcg atcacatcca gggccagccc
     1561 gcccatatgg ctcacgtcga tgacgatccc cagccgattg cattcggcga tcagcgcccg
     1621 cccgaacgcc gtcaactggc cgttgacgat cgcctggttc cccgccggcc accagagctg
     1681 gatctcgcgc gtccccaacg cgtgaaactc ctccagcgct tcaagctcgc cctccagata
     1741 acgcaccccc tccgaaccga tgatcgcccc gatcttcccc tcgaccttcg cctgatacag
     1801 atcctccacg gtccggatga tcgtcacgtt cgcttccggg ttgtccgcca gcgcctgcac
     1861 cccctccaga taccccaaat gccgctcgcg ccagccctcg taactcgtta cctcgtagcg
     1921 ctggccattt tcccagtcga ttccgtcggt cgtcagcttc aggaccttcg ccgtcaaccc
     1981 cgcctcgcgc gcggcgtcga agtcccccgc cgtgaacgta tggtcgtggg ccagaatgat
     2041 gatcgacgaa ttgtgcagcc gctcggcgcg ctcctcctcc gtctcctccc cccacacgat
     2101 ccggtacagc cgcaccagat tgagccccag gatcttctcg atctcctcgt cgctgtagcc
     2161 gcgttccacg agcccgcgcg tcagctccag catctcctcc gcatccagcg cgaaggtcgt
     2221 ccccgtctcc ggataccagt ccgcgccgat cccgacatgg tcgacgccca ccaggttgac
     2281 gatgtaatcg atcgcatcga ggaccttcgt cagcggttgc aggtagccga agtgaacgca
     2341 gaacacgccg ttgcccccgc cgctgttggc caccagttgg atcagcgcat ccgaggcctc
     2401 gccccccatg tgcgccggcg cgtcatgcga tcgcaccacc ggatcctcgg tgttcgccat
     2461 caccgccggc acttcgctgc cgtacagatg gctgatgtcg atcagaacgc ccagccggtt
     2521 gcattccgcg atcacctgcc ggccgaactc gctcaggctg ctcccgtcca gaagctggtt
     2581 ggccgcccat cgcaattggg tctcgcgcca gcccaggctg tggaaccgtt ccacattggc
     2641 cagttcgccc tccaggtgca tcgcgccttc cgagccgatg atcgccccca cctccccggc
     2701 cgctttggcg gcgaaaatgt catccaccgt ttcaatcacc cggaccttgt tttccgggtt
     2761 gtccgcgatc tggcggatgt cccccaggta gcccaggaac cgctcggtcc acccctccag
     2821 ccccggcacc gggtagcgcg tccgcgtgtc gcggttccag tcaatgctgt cggtgatcag
     2881 cttcagcacg acggccgtga tcccgccggt gtgcgcgtcg gcgaagtcgg ccgccagaaa
     2941 atcgtgcgag tgcgccacga tcaccagcga ctcggcgtgc agccgctgcg cacgttcttc
     3001 ctcctccggc gtcagcttga tcgcctcgac cgcggccttt gccgcggccg gcgccggccg
     3061 ctcccagtcg atcgggcccg cgcccggcgg cgccgccaac ggccgcacca gcgcgccgac
     3121 cagaccggcc gcgagggcgg cgcgccgcag cgcgcgagcg acgatggggg ccacaacgtg
     3181 catgggaaat cgaataccgg aaatcattat ccggtaagac ggcccccgcg acaagcggaa
     3241 aacgccctcc ccccgcgccg cctggtcgtc ccgccgccgt tatcctgctg aaggctgaaa
     3301 tcccgctgcg gtctcgctgc aagcgagttc gcgcgtcacg ccccctcttt tcttcccctc
     3361 ttccctcgcc aaaatttccc ttgacgcgaa atcggtttcc gttaaacaat atccctacaa
     3421 ccgaatggtt cgataacttc tccggagccc cggctcacat caccctcttt gaacaccgcg
     3481 gccgcgtcgg ccccgcgccg atgccgcttc gcaacagcct gcgtccgatg gctctctccc
     3541 gccgcccagc gcccctgctc ccggctcccg cccggttcgc gcccatcgac gctccgccgg
     3601 ccgtccgctc acgcgccgcc gattccgtca ctccgaaaag gagatcgctg gttcgttttc
     3661 cgttgttgtc cggacgcctg gcgtgtccgt tgccaatttt gggaggaggc tctctcatat
     3721 gagactcgcc aactttttgc tctcactggc ggcgcttgct ttgctgttcc tcgcgtcgcc
     3781 tcccgccggg gctcagccca ttctgatcga tagcaacgcc aatgccgtgg ccgaggatct
     3841 cgccctcggc aactttggcc aggatgccca tgccggggtc gtcgccgcct ggcgcgacag
     3901 cgcctccgaa gatcttgcga tcgactttta ccgctacgag gaacttccct cgtccaactg
     3961 ggccgccgag cgcatcgccc aagtgagcga cgtcgccacc agccccaccg attcccccgc
     4021 tccggcggtc cgaatcgtgc ttggagacta cagccgggat ggcaagaccg acctgaccgt
     4081 catgtacaac tccaacctca ccaccaccag cttgaagcac tatcagcgca ccagccccgg
     4141 cttcg
//
LOCUS       DAOPCO010000335         4102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_17829_length_4102_cov_4.322461, whole genome shotgun sequence.
ACCESSION   DAOPCO010000335 DAOPCO010000000
VERSION     DAOPCO010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17829_length_4102_cov_4.322461"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLS90_15855"
     CDS             complement(<1..293)
                     /locus_tag="PLS90_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03922.1"
                     /translation="MDSRERIKRIIAGQPADRCGFWLGNPAPQSWPILHRHFGTSEDE
                     ELRRLLGDDFRWICPQFFPSTYRHPDGRAMFHLGADKKVSHGQAGPFAECEDLA"
     gene            complement(515..1057)
                     /locus_tag="PLS90_15860"
     CDS             complement(515..1057)
                     /locus_tag="PLS90_15860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPK03923.1"
                     /translation="MYRLEWTVKWLALLLVTITLCGCARKMTTPKNVIMDHLSNAAQY
                     SDLHSGFEQAFEYLTANDMKAMQPGEYAIDGDRIFCIVSQGEARSHEEANLEFHRKYI
                     DIQYVIEGTDIMGWKPMDQCRKVLHPYDPAKDIGFYEDRPVLWQKVRAGDFIIFFPDD
                     AHAPFVGQGEIKKAVIKVAR"
     gene            complement(1121..2731)
                     /locus_tag="PLS90_15865"
     CDS             complement(1121..2731)
                     /locus_tag="PLS90_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPK03924.1"
                     /translation="MNEIAHKFHFLDMIAIAAYFLLMMAIGIYFARRNRNTDDYFLGN
                     RSIPGWALGLSMMGAIISSITFLALPAGAYALDWRMILPNLMLPVSLLFAIFIFIPLF
                     RAGISASAFEYLEMRYGSIARLYGAVSFIILQVLRIGMVLYLVALPLKIMLGVPMLWV
                     ILVCGVITIIYTASGGFEAVVWTDVIQAVILLLGGVVCFTIILLKLPGGLGQVIDVGM
                     QYKKFSFGEVEFNLLERTMLTMIITGTWAWIAQYSSDQNVVQRYLAAPSLREARKATV
                     LCAATCIPTWFFFFFLGTCLFVYYKVNPSSIVQSMEGANENDGVLPYFIVTEIPAGIM
                     GLIIAGVLAAAMSTLSAGINAISTVTVSDFFRRYLVPGRDEKFYLDLARYTSLCAGGL
                     MIAFALIFWKLPKESMMDVNLIISAVFGGALSGLFLIGLFTRRVHNRAMLLGLGLAVA
                     FNIYLMLDSMMNLIPSPFSLGVHAYWVALLVNILFMVSAYAASFVLKSDPKDIHGLTI
                     WTIDKNGQAVDRKPRKATAPLEWAHGED"
     gene            complement(2751..>4102)
                     /locus_tag="PLS90_15870"
     CDS             complement(2751..>4102)
                     /locus_tag="PLS90_15870"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HPK03925.1"
                     /translation="TIFYNSGHIGPNHLQSIKSYTHWELESLPSGGWGYLHFPIAMRY
                     ARQYLPSVGMTGKFHSSWGDFNSYKNKTALEYECFQALALGAGCSIGDQMHPSGQLSP
                     ATYELIGSVYSAVARKEPWCREARAMADIAVVTPEACDPLITRNALPMSIFGVTRMLQ
                     ETQQQFDIIDLNADFEAYKLVVLPDDIRLTRDEAAKLDRYVQEGGSIIASHRSGMDKQ
                     ADEFVLKALNVDYVREAPYEPDFICAKPPLDRGLPGEPLVMYSRGLQVAPRADGRIAA
                     VVQRPYFNRTWRHYCSHMHAPSSGEVVYPGIVASPRCIYFAHPIFTQYQKNTPRWYKQ
                     LFVNAVESFLAAPLIRVFNAPSTLLVSINVQPKLNRYIVHLLHYIPERRGTEFDIIED
                     VIPLHDLEIGVHVPHAVTAVAMVPEKVPLDFRTLDGGIRVCVPKLAGHQMIELAYET"
BASE COUNT          961 a         1174 c         1169 g          798 t
ORIGIN      
        1 gcaaggtcct cgcattcggc gaagggcccc gcctggccgt gcgaaacttt cttgtccgcg
       61 ccaaggtgga acatcgcgcg cccatccggg tgccggtagg tcgagggaaa aaactgcggg
      121 cagatccagc gaaaatcgtc gcccagcagg cggcgcagct cctcatcctc gctggtgcca
      181 aagtggcggt gcaggatcgg ccagctctgg ggggcgggat tgcccagcca gaacccgcaa
      241 cgatcggcgg gctgacctgc aataatgcgt ttgatgcgct cgcgcgaatc catgacaagc
      301 cttcctgttt ggtttcgatc agccgcccgg cctatgaagc ccgccgatcg ggatgacacg
      361 gctgcttgcg gcggtcacgg gcgcgtgggc gacgccggga ccgcagcggg gctgccgcaa
      421 gctcgacccc tgccgcctga tgcgcgttcg gatctggcac aatgttaaga tcaggacgca
      481 gcggcacttc accgggcaat cgcgtatccg ctcatcagcg ggcaaccttg atgaccgcct
      541 tcttgatttc tccctgtcct acgaatggcg catgggcatc atccggaaag aagatgatga
      601 aatctccagc tctcactttc tgccacagca ccggtcgatc ttcataaaat ccaatgtcct
      661 tcgcggggtc gtaggggtgc agcactttcc ggcactgatc catgggcttc cagcccataa
      721 tatcggtccc ctcgataaca tactgtatat caatgtactt ccggtgaaat tcaagattgg
      781 cctcttcatg ggagcgcgct tccccttgac tgacaataca gaaaatccga tcgccgtcga
      841 ttgcatattc accgggctgc atcgctttca tatcgttcgc cgtcagatac tcaaaagcct
      901 gctcgaatcc agaatggagg tctgaatatt gagccgcgtt tgacaggtga tccatgatca
      961 cgtttttcgg ggtcgtcatt ttgcgtgcac atccacataa agtgatcgta accagcaaga
     1021 gcgccagcca tttcactgtc cattcgaggc gatacataac gttatccctc ttgatcaaga
     1081 taaacaatcc actcaccaga actcaatacg caatacgccg tcaatcttca ccatgcgccc
     1141 attcgagggg agcggttgcc ttgcgcggct tccgatcaac ggcctgtccg ttcttatcaa
     1201 ttgtccagat ggtcaaacca tgaatatcct tggggtcgct cttaagcaca aaactggccg
     1261 cataggccga caccatgaac aggatattca ccaacagcgc cacccagtaa gcgtgaaccc
     1321 ccaggctgaa gggggacgga atcagattca tcatgctgtc gagcatcaga tagatattga
     1381 acgcaacagc gaggccgagt cccagcagca tcgcgcggtt gtgcacgcgc ctggtgaaca
     1441 ggccaatgag gaacagaccc gaaagggccc cgccaaacac cgcgctaatg atcaggttca
     1501 cgtccatcat gctctctttg ggcagcttcc agaaaatgag ggcgaacgcg atcatcagac
     1561 cgccggcgca cagcgaggta tagcgggcca gatccaggta gaatttctcg tcgcgtccgg
     1621 gcacgaggta acggcggaaa aaatcggaca ccgttaccgt cgagatggca ttgattccgg
     1681 cggatagggt cgacatcgcg gcggccagaa cgccggcaat gatcaggccc atgatgcccg
     1741 ccggaatttc ggtgacaata aagtagggca gcaccccgtc attctcattg gccccttcca
     1801 tcgactgcac gatcgagctg gggttgacct tatagtacac gaacaggcag gtgccgagaa
     1861 aaaagaaaaa gaaccaggtc gggatgcagg tagcggcgca gagcaccgtc gccttgcgcg
     1921 cctcgcgcaa tgagggtgcg gcgaggtagc gttgcacaac attctggtcg ctcgaatact
     1981 gggcgatcca ggcccacgtg ccggtgatga tcatcgtaag catcgttcgt tcgagcaggt
     2041 tgaactcaac ttccccgaaa ctgaatttct tgtattgcat gccgacgtca atcacttgcc
     2101 ccaatccgcc gggcagttta agcaggataa ttgtgaaaca gacgacgccg cccaggagca
     2161 gaatgacggc ctggatcacg tccgtccaga ccaccgcttc gaagccgccg ctggcggtgt
     2221 agatgatcgt gatcacgccg cacacgagaa tgacccagag catcggaacg ccgagcatga
     2281 tcttaagcgg cagggctacg aggtagagca ccatgccgat gcgcagcacc tgaagaatga
     2341 tgaaactgac ggccccatac agtcgcgcaa tgcttccgta ccgcatttcg aggtattcaa
     2401 acgccgaagc cgaaattccc gctcgaaaga ggggaataaa gataaagatc gcaaacagca
     2461 gcgaaacggg cagcatcagg ttgggtagaa tcatgcgcca gtcgagggca taggcgccgg
     2521 ccggcagggc aagaaaggtg atcgaactga tgatcgcgcc catcatcgag aggcccagcg
     2581 cccaaccggg aattgatcgg ttgcccagga agtaatcgtc cgtgttccgg tttcgccggg
     2641 caaaatagat gccgatggcc atcatcagca aaaaatacgc ggcaatggcg atcatatcca
     2701 ggaagtggaa cttgtgggcg atttcgttca ttgcgccgtg ctccgggggt tcacgtttcg
     2761 taagctaatt cgatcatttg atgcccggcg agctttggta cgcaaacccg aataccgccg
     2821 tcgagggttc taaagtcaag aggaaccttt tcgggaacca tggcaaccgc ggtgaccgca
     2881 tgcgggacat ggaccccaat ttccaggtcg tgtagcggga tcacatcttc gatgatgtcg
     2941 aactccgtgc cgcgccgttc gggaatgtaa tgcagcaaat gcacgatata gcggttcaat
     3001 ttaggttgga cgttgatgga aaccagcaag gtactcggcg cattgaagac ccgaatcaac
     3061 ggcgccgcga gaaaggactc aaccgcattg acaaacagtt gtttatacca gcgcggcgtg
     3121 ttcttctgat actgcgtgaa gatcggatgg gcaaagtaga tgcaacgcgg gctggccacg
     3181 atgcccggat aaacaacttc gccgctggag ggtgcgtgca tgtgcgaaca gtagtgtcgc
     3241 caggtccggt tgaagtaggg gcgctgcaca acggctgcaa tccggccgtc cgcgcgcggc
     3301 gccacctgga gcccgcggct atacatcacc aacggttcac cgggcaatcc gcggtccagc
     3361 ggcggcttgg cgcagatgaa atcgggctca tacggcgctt cccgcacata gtccacattc
     3421 aacgccttga gcacaaattc gtccgcttgc ttgtccatac cacttctgtg ggaagcaatg
     3481 atcgaaccgc cctcttgcac atagcggtcc agtttggccg cctcgtcccg ggtcaaccgg
     3541 atgtcgtcgg gaaggacgac cagcttgtat gcctcgaaat ccgcattcag gtcgatgata
     3601 tcgaactgct gctgggtctc ctggagcatc cgcgtgaccc caaagatcga catcggcaag
     3661 gcattgcggg taatcagcgg atcgcaggcc tcgggcgtca caaccgcaat gtcggccatc
     3721 gcccgcgcct cccggcacca gggttccttc cgtgccacgg cgctatagac cgagccgatc
     3781 agctcatagg tggccggaga gagttggccc gagggatgca tctgatcgcc gatcgaacaa
     3841 ccggcgccca gcgcaagcgc ctgaaagcac tcgtactcca gggcagtctt gttcttatag
     3901 gaattgaaat cgccccagga tgaatgaaac ttgcccgtca tgccgacact gggcaggtac
     3961 tgacgcgcat aacgcatcgc gatgggaaag tgcaaatagc cccaaccgcc cgagggcagc
     4021 gattcgagtt cccagtgggt gtaggacttg atggattgga ggtgattcgg tccgatatgg
     4081 cctgaattgt agaaaatcgt gc
//
LOCUS       DAOPCO010000336         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_17919_length_4085_cov_4.228040, whole genome shotgun sequence.
ACCESSION   DAOPCO010000336 DAOPCO010000000
VERSION     DAOPCO010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17919_length_4085_cov_4.228040"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(518..1777)
                     /locus_tag="PLS90_15875"
     CDS             complement(518..1777)
                     /locus_tag="PLS90_15875"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK03926.1"
                     /translation="MDYLTVGALWARYGLDAEGWRDGILLRWRDGNVVACSPAPRPPA
                     DVDPAWRFDSHLLTPGLLNAHCHLDYSLLRGRLSGAAGFAAWLRQILAARRGLDDSAA
                     EPARAAAAAALDELIHDGVTEIWDVASFGWAVPLLRARNVPAIIFREWIAPDPARAAA
                     FDAWANATFTESDPAPTDGISPHAPYSVCAEGLRRADAWARRHARPVAIHLAESPEER
                     ELLLAGTGALWKLLSEIAGRDVAQTLGRGNSPIARAEEAGLIRPETLAIHANLPEPGE
                     PERLARVGCRVVFCPRSHAFFGYPRYPLKTWRAAGVGLALGTDSLASNDRLSIRAEAR
                     SLVAAHGWLRPADALACATGAWLGAAPPFGGRGRFTPGAPAHWALWKIAPPPASLPAD
                     RCADDLASQWLHGSAPLAASSLLPPIH"
     gene            complement(1765..2868)
                     /locus_tag="PLS90_15880"
     CDS             complement(1765..2868)
                     /locus_tag="PLS90_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPK03927.1"
                     /translation="MSRERRILIVDDEQRMATSLCALLTQCGYRCTAALSGREALELL
                     DREPVDVIVSDLRMPGLDGLEFIRQAHERHPRTLVIVITGHATTDSAIEAVRHGVFDY
                     LRKPFEFDLLRMAVEKAFARIDADRFREDVAAMITHDIKIPLTSIIGFASMIHESGEG
                     ELHPRSREFAQAIHANGQKVLELIENYLTSCKIEAGTLRVLRSRINLKAALHDVVEAL
                     HVVAARRGRRILTHLDEIPEFAPLDQVLLYRVFANLLTNAIRYSRGEEPILVEARALD
                     GEPPMLRLTLTNDMDQARPEDFAHVFERYQRSVATQSLEGVGIGLYVVKAAVQAQGGR
                     VDVEVPDPHHVRFTAWLPLVEEGLPVEEQPWTT"
     gene            complement(3004..3747)
                     /locus_tag="PLS90_15885"
     CDS             complement(3004..3747)
                     /locus_tag="PLS90_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03928.1"
                     /translation="MIYYASRLTQPLVPIFFVACLALTLACGPAGDAPDSSATDAGAA
                     GQAMQSPTADSTGQPDEGAPAPDATPADSADPATSPATPQPATERKYPPFVGDNLTTE
                     AMLQHLGEPGVGVIAWKTQSEENNFGFNVLRSENEEGPFVKVNRNPILGAGNSSTENR
                     YVYYDKTVKIGDVYSYYLESISMGGVREQFSPVITDVIRRPYYAELDDSPPVDDVLPE
                     GLDVPTTPATPVDVVPADAAQPAEQPSGD"
BASE COUNT          684 a         1087 c         1704 g          610 t
ORIGIN      
        1 gagggggcca gtagaaagtc cgccggcgcg ggctcgaagg acgccggctg gggatggagt
       61 tgagcggaca gggatggaat gccatggact ggatggacgg ggtggaataa gtggacgggg
      121 ttgattgggg ggttgggggg gggatcggca agaggttgag ccgattgcga acagaagcgt
      181 gggttggctt gctttcgaga gccgaggacg agggacgaga gccgagagcc gaatcttggg
      241 gagggcctct gggggcgatt acgagtcgct catgaacctg cgtttcacaa cgagagatga
      301 aatcgggggg gggtgagcag ccaattcgaa cagcccaggc cttgccggtt ccttcagcag
      361 ggcagtccca tcagggacgg cagacttcag cccgccagtt cactggcggg aaccgggata
      421 cccatcgcgc ccgagtcccg gtgggacggc agaaccgatc caagtatccc gagaataggg
      481 acaaatttcc gagtgaggtc gaggacgagg gatgaattca gtgtatgggg gggaggagag
      541 aggaggcggc gaggggggcg gagccgtgga gccactggct ggcgaggtcg tcggcgcagc
      601 ggtcggcggg caggctggcg ggcggcggcg cgattttcca gagggcccag tgcgcggggg
      661 cgcccggagt gaagcggccg cggccgccga aaggcggggc cgcgccgagc cacgcgcccg
      721 ttgcgcaggc gagggcgtcg gcggggcgga gccagccgtg cgccgcaacg agtgagcgcg
      781 cctcggcgcg gatcgacagc cggtcgttgc tggcgaggct gtccgtcccc agggcgaggc
      841 cgacgcccgc cgcgcgccag gttttgagcg gatagcgcgg atacccgaag aaggcatgac
      901 tgcgggggca gaagacgacg cggcagccga cgcgggcgag gcgttcgggc tcgcccggct
      961 cgggcaggtt ggcgtggatc gccagggttt cggggcggat gaggccggct tcctcggcgc
     1021 gggcgatggg ggagttgccc cggccgaggg tttgggcgac atcgcggccg gcgatctcgg
     1081 agagcagttt ccacaaagcg cccgtgccgg cgaggaggag ttcgcgttcc tcgggcgatt
     1141 cggccagatg gatggcgacg ggacgggcgt gccggcgggc ccaggcatcg gcgcggcgca
     1201 atccctccgc gcagacggaa tagggggcgt ggggcgagat gccgtcggtc ggcgcggggt
     1261 cggactcggt gaaggtggcg ttcgcccagg catcgaaggc ggcggcgcgg gcgggatcgg
     1321 gggcgatcca ttcccggaag atgatggcgg ggacgttgcg cgcgcggagc agggggacgg
     1381 cccagccgaa gctggcgacg tcccagattt cggtgacgcc gtcgtggatc agttcgtcga
     1441 gggcggcggc cgcggcggcg cgggccggct ccgcggcgga gtcgtcgagg ccgcggcgcg
     1501 ccgcgaggat ctggcgcagc caggcggcga accccgccgc gccggagagg cgtccccgga
     1561 gcagcgaata atcgagatgg cagtgggcgt tgagcaggcc gggcgtgagc aggtggctgt
     1621 cgaaacgcca ggcggggtcg acgtcggcgg ggggacgggg ggccgggctg caggcgacga
     1681 cgttgccatc gcgccagcgc aggaggatgc cgtcacgcca gccctcggcg tccagaccgt
     1741 agcgggccca gagcgcgccc acggtcaagt agtccacggc tgctcctcca cggggaggcc
     1801 ttcctcgacc agcggcagcc atgccgtgaa gcgaacgtgg tgggggtcgg ggacttcgac
     1861 gtcgacgcgt ccgccctggg cttggaccgc cgccttgacg acatagaggc cgatgccgac
     1921 cccttcgagc gactgcgtgg cgacggagcg ctgatagcgt tcgaagacat gggcgaaatc
     1981 ctcggggcgg gcctgatcca tgtcgttggt gagggtcagg cggagcatgg ggggctcgcc
     2041 gtcgagggcg cgggcctcga cgaggatcgg ttcctcgccg cgactgtagc ggatggcatt
     2101 ggtcagcaga ttggcgaaga cgcggtagag caggacctgg tcgagcgggg cgaattcggg
     2161 gatctcgtcc aggtgggtga ggatgcggcg gccgcggcgg gcggcgacga cgtggagcgc
     2221 ctcgacgacg tcgtgcaggg cggccttcag gttgatgcgg gagcggagga cacgcaaggt
     2281 ccccgcctcg atcttgcagg aagtcaggta attttcgatc agttcgagga ctttctggcc
     2341 gttggcgtga atcgcctggg cgaattcgcg gctgcgcgga tggagctcgc cctcgccaga
     2401 ctcgtggatc atcgaggcga aaccaataat ggaggtgagg gggattttga tgtcgtgggt
     2461 gatcatcgcg gcgacatcct cgcggaagcg gtcggcgtcg atgcgggcga aggccttttc
     2521 gacggccatg cggagcagat cgaattcaaa cggcttgcgc aggtaatcga agacgccgtg
     2581 gcggacggcc tcgatggccg aatcagtggt ggcatgaccg gtgatcacga tgacgagggt
     2641 gcgcgggtgg cgttcgtggg cttgacggat gaattccagg ccgtccaggc cgggcatgcg
     2701 cagatccgag acgatgacgt cgacgggctc gcggtcgagg agttcgaggg cctcgcggcc
     2761 gctgagggcg gccgtgcagc gatagccgca ctgggtgagc agggcgcaca gggaagtggc
     2821 catccgctgc tcgtcgtcga cgatcaggat tcgcctttca cgggacatga tgcgccgccg
     2881 tgcccgaaac gggagatggg acgattgtgg tgccgcggga gggaaaggga aagggaaaaa
     2941 gtcgggggcc ctggggccga ttaagaggac gggggacgga gcttgtcgag gctcggcgcg
     3001 gggtcagtcc ccggagggtt gctcggcggg ttgggcggcg tcggcgggga caacgtcgac
     3061 cggggtggcg ggggtggtcg gcacgtcgag gccttcgggg aggacatcgt cgacgggcgg
     3121 ggagtcgtcg agctcggcgt agtagggtcg gcggatgacg tcggtgatga cggggctgaa
     3181 ctgttcgcgg acgccgccca tggagatcga ttcgagataa taggaataga cgtcgccgat
     3241 cttgaccgtc ttgtcgtagt agacatagcg gttttcggtg gagctgttgc cggcgccgag
     3301 gatcggattg cggttgacct tgacgaaggg cccttcttcg ttttcggagc gcaggacgtt
     3361 gaaaccgaaa ttattttcct ccgactgggt tttccaggcg atgacgccga cacccggttc
     3421 gcccagatgc tggagcatgg cttcagtggt cagattatcg ccgacgaagg gagggtattt
     3481 ccgttcggtc gcgggctggg gggttgcggg ggaggtggcc ggatcggcgg agtcggcggg
     3541 ggtcgcgtcc ggcgccgggg cgccctcgtc gggctggccg gtcgaatcgg cggtcgggga
     3601 ttgcatggcc tgaccggccg cgccggcatc ggtggcggag gaatccgggg cgtcgccggc
     3661 cgggccgcac gcaagcgtca gcgcgaggca ggcgacgaag aagatcggaa cgagcggctg
     3721 cgtcagccgt gaggcgtagt agatcatgga ccaggtctct cgaggtgatg cgtccggccg
     3781 gcgcccacgt cgcattgcga acccacatcc cgacggccga atcggcagac tgcggaccat
     3841 gatttggttt gcgcgcgggc aaagtcaagc gaaagcgggc gagtgcgagg gcaagtgcag
     3901 gtagatcctg acccccccgt gggcgggcgc gggcgagtgg aggggggatg gagggcggga
     3961 gcggagggcg tcaggcggaa aaggcaagac cttggaatgg cggcgtggcg ggggggaacc
     4021 gttgaaacgg ttagaagaaa ttgtctcgtg gagtcaccgg gccgaggcct ggtgctaatg
     4081 acggg
//
LOCUS       DAOPCO010000337         4045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18137_length_4045_cov_4.090977, whole genome shotgun sequence.
ACCESSION   DAOPCO010000337 DAOPCO010000000
VERSION     DAOPCO010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18137_length_4045_cov_4.090977"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PLS90_15890"
     CDS             <1..1577
                     /locus_tag="PLS90_15890"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPK03929.1"
                     /translation="GVSNFAPHQLAPLHRRLALATHQIELSPLEITPFMNGLTDQALA
                     RRMTVMAWSPLGGGRLGDPNLFACWLAVLAVFGAGCLAPGERAAWRAAGGLSTAACAG
                     ALLLTGSRGGLLGLVLGGALAGYSVGRQVWPPRARRAGLMLIGLIGLIGLLAWMEANP
                     AGWAGGPTAAAAEAQAQPEGRSRLEAPAPEEGGWLERLGNIETIRERLYYWQIALRVW
                     QRQPLVGAGPGGFSLLYPTLKPSAARESRYAHNWLFEALSELGLVGAGLLSAFWVLLA
                     RRYLRVARMPRGGSLPEALWPLIALAVLCAAGLVQFVMQWREFLILIGVLAGLAGGAT
                     RRALSREQFDARTALTWGAGCCVMLLAILMTPRLRAATYYEWRGKDLMQAGDYAAADE
                     AFMRAAELLPDDAGLWVNVAAAKSRTKERSTAWPYLSRAAELNPYSASVCAAQARWLA
                     ERGRLVEAIVKLDHAVERYPSNVEYRLERAALLIQAERPEAAREDLRFIAEERLPLWE
                     YQEPTYEALKSALGAP"
     gene            complement(1632..2828)
                     /locus_tag="PLS90_15895"
     CDS             complement(1632..2828)
                     /locus_tag="PLS90_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPK03930.1"
                     /translation="MIQRHLTSALRQAAQRYPIVTLTGPRQSGKTTLVRATFPMHPYI
                     SLEDPDRRAFALEDPRGFLRQFDRGAVLDEVQRVPDLFSYLQTNVDADDTPGRFILTG
                     SQNFLLMQKISQSLAGRSAILHLWPFTRSELLDRESLAPRSIGRALPRSHTTGDDLFD
                     VMWRGFYPRIHDKQLPAQDWLANYTRTYIERDVRQLLNVGDLETFGRFLRFCAGRSGQ
                     LLNISSLAADCGVTHSTARRWLSVLEASFLILLLRPHFRNFNKRLVKSPKLYFLDPGL
                     LCYLLQIRSADELRFHAARGAVFETFVISELFKDIQHRAEQLSLYFWRDSTGHEIDLI
                     LDQGSHLTAIEIKSGETLAADFFEPLRFWRALPDQTEAPAALVYGGSESLSRNQVTVY
                     SWDAWP"
     gene            2999..3955
                     /gene="murQ"
                     /locus_tag="PLS90_15900"
     CDS             2999..3955
                     /gene="murQ"
                     /locus_tag="PLS90_15900"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HPK03931.1"
                     /translation="MTQETDPTPAPELPPARHAARLTEQRNPRSMRIDRASIREALAI
                     IHAEDRRCVEAAEACAPEVARAIERVVASFEAGGRLFYVGAGTSGRLGVLDASEQPPT
                     FGVAPTMVQGIIAGGYEALHRSVEGAEDDPSAGAAAIAERGIDARDTVCGITTGGRAP
                     YVLGSLAEARRRGAGTILLTCTPPLEGEEELADVQIHALVGPEVVTGSTRMKAGTVTK
                     LILNQITTVAMIRIGKVYENLMVDVRPTNRKLLDRAVRIVMTLTGLGREGAAELLERA
                     GRDVKVALVMQRAGVGAEEARRRIAAAGGHVARAIAATGAGE"
BASE COUNT          638 a         1088 c         1684 g          635 t
ORIGIN      
        1 tcggcgtcag caacttcgcg ccgcaccagc tggcgccgct gcaccgccgc ctggccctgg
       61 ccacgcacca gatcgagctg tcgccgctgg agatcacgcc cttcatgaac ggcctgaccg
      121 accaggcgct ggcccgccgc atgaccgtca tggcctggtc gcccctgggc ggcggccggc
      181 tgggcgatcc gaatctgttc gcctgctggt tggcggtgct ggcggtgttc ggtgcgggct
      241 gtctggcgcc gggggagcgg gcggcctggc gcgcggcggg ggggctgtcg acggcggcgt
      301 gcgcgggggc gttgctgctg acggggagcc gcggggggct gttggggctg gtgctggggg
      361 gggcgctggc gggctattcg gtggggcggc aggtgtggcc gccgcgggcg cggcgggccg
      421 ggctgatgct gatcgggctg atcggactga tcggactgct ggcctggatg gaggcgaacc
      481 cggccggctg ggccggcggt ccgaccgccg ccgcagccga ggcgcaggcg caaccggagg
      541 gacggtcgcg gctggaagcg ccggcgcccg aggagggcgg ctggctggag cggctgggca
      601 atatcgagac gatccgcgaa cggctttatt actggcagat cgcgttgcgg gtgtggcaac
      661 ggcagccgct cgtgggagcg ggaccgggcg gattttcgct gctctacccg acgctgaagc
      721 cgtctgccgc gcgcgagagc cgctatgcgc acaactggtt attcgaggcg ctgtcggaat
      781 tggggctggt gggcgccggg ctgctgagcg cgttctgggt gctgctggca cggcggtatc
      841 tgcgtgtggc gcggatgccg cgcggcggat cgctgcccga agcgctctgg ccgctgatcg
      901 cgctggcggt gttgtgcgcg gcggggctgg tgcagttcgt tatgcagtgg cgcgagttcc
      961 tgatcctgat cggcgtgctg gcggggctgg cggggggggc gacgcgccgg gcactgagcc
     1021 gcgaacaatt tgatgccagg accgcgctga catggggcgc ggggtgctgc gtgatgctgc
     1081 tcgcgattct gatgacgccg cggctgcggg cggcgacata ttatgaatgg cggggcaagg
     1141 acctgatgca ggccggggac tacgcagcgg cggacgaggc gttcatgcgg gcggcggaac
     1201 tgctgcccga cgacgccggg ctatgggtga acgtggcggc ggcgaaatcg cggacgaagg
     1261 agcggtcgac ggcgtggccg tatctgtcgc gggcggcgga gttgaacccc tattcggcgt
     1321 cggtttgcgc ggcgcaggca cgctggctgg ccgaacgggg gcggctggtg gaggcgatcg
     1381 tcaaactgga tcacgccgtg gagcgctacc ccagcaacgt ggaatatcgg ctggagcggg
     1441 ccgcgctgct gatccaggcg gagcggccgg aggcggcgcg ggaggatctg cggttcatcg
     1501 cggaggagcg gcttccgctg tgggagtacc aggagcccac ctatgaggcg ttgaagagcg
     1561 cgctgggggc gccgtgaggg cgggggcagg ggatgtgagg gacatccggg ggcgagaggc
     1621 cgtggggttg atcaaggcca tgcatcccag gaatagaccg tgacctgatt gcggctgagg
     1681 gattcggagc cgccatagac cagggcggca ggggcctcgg tctggtccgg gagtgcgcgc
     1741 cagaaacgca ggggctcaaa gaaatcggcg gcgagggtct cgccggactt gatctcgatg
     1801 gcggtcaagt gcgatccctg gtccagaatc aggtcgatct catggccggt cgaatcgcgc
     1861 cagaaataga ggctgagctg ctcggcgcga tgctggatgt ccttgaacag ttcggagatc
     1921 acgaaggtct cgaagacggc gccgcgggcc gcatgaaagc gcagttcgtc ggccgaccgg
     1981 atctggagca gatagcagag gagcccgggg tcgaggaagt agagtttggg cgatttgacg
     2041 agccgcttgt tgaagttacg aaagtggggc cgcaggagca ggatgaggaa actggcttcg
     2101 aggaccgaga gccagcggcg ggccgtcgaa tgcgtcacgc cgcagtcggc ggcgagcgag
     2161 gagatgttga ggagctgtcc gctgcgaccg gcgcaaaatc gcaggaagcg accgaatgtt
     2221 tccagatcac cgacgttgag cagttggcga acgtcgcgct cgatgtaagt gcgggtgtaa
     2281 ttggcgagcc agtcctgggc cggcagctgc ttatcgtgaa tccgcggata gaagccgcgc
     2341 cacatgacgt cgaacagatc gtcgccggtt gtgtgcgagc gcggcagcgc gcgaccgatt
     2401 gatcggggcg ccagggattc gcggtcaagc agttcggaac gggtaaacgg ccacagatga
     2461 agaatggcgg agcgcccggc caggctctgg ctgatcttct gcatgaggag gaagttctgc
     2521 gagcccgtca ggatgaagcg gccgggcgta tcatcggcat cgacattggt ttgaaggtag
     2581 gaaaataagt cgggaacgcg ctggacctcg tccaggacag cgccccggtc gaactgacgc
     2641 aggaagccgc gcgggtcctc cagagcgaaa gcgcgccggt cgggatcctc cagtgaaatg
     2701 taggggtgca tggggaaggt ggcgcggacg agggtcgttt ttccggattg acgcgggccg
     2761 gtgagcgtga caatgggata gcgttgggcg gcttggcgca aggcggaggt gaggtgacgc
     2821 tggatcaatt ggcggcctcc atttgtttac attttgaata ttcaaaattc aatttgcaag
     2881 ccccgatttg aaaagccgtg gctcggcctc gcccttgcgg tgtggttctg gtgctccgtc
     2941 gatcggtctt ggaaaccgcg ggcgggcgcg ataaggtttg cggcggaaag gcggcggcat
     3001 gactcaggag accgacccca cgcccgcccc ggaactgccg cccgcgcggc acgcggcgcg
     3061 gctgaccgag cagcgcaatc cgcgttcgat gcggatcgac cgggcgtcga tccgcgaggc
     3121 gctggcgatc atccatgcgg aggatcgccg ctgcgtcgag gcggccgagg cgtgcgcgcc
     3181 agaggtggcg cgggcgatcg aacgggtcgt ggcgagcttc gaggcgggcg ggcggctgtt
     3241 ttatgtgggg gcggggacgt cggggcggtt gggggtgctc gatgcgagcg agcagccgcc
     3301 gacgttcggc gttgcgccga cgatggtcca ggggatcatc gccggggggt atgaggcgct
     3361 gcatcggtcg gtcgaggggg cggaggacga tccgtcggcg ggggcggcgg cgatcgcgga
     3421 gcgcgggatc gacgcgcgcg atacggtctg cgggatcacg acgggggggc gcgccccgta
     3481 cgtgctgggg tcgctggccg aggcgcggcg gcgcggggcg ggcacgatcc tgctcacgtg
     3541 cacgccgccg ctggagggcg aggaggagct ggccgacgtg cagatccacg cgctggtggg
     3601 gccggaggtg gtgaccggct cgacgcggat gaaggcaggg acggtgacca agctgatcct
     3661 gaaccagatc acgacggtcg caatgatccg gatcgggaag gtgtatgaga acctgatggt
     3721 ggacgtgcgg ccgacgaacc gcaagctgct ggaccgggcg gtccggatcg tgatgacgct
     3781 gacgggtctg gggcgcgagg gggcggcgga gttgctggag cgggccgggc gcgatgtgaa
     3841 ggtggcgctg gtgatgcagc gggcgggggt tggggcggag gaggcgcggc ggcggattgc
     3901 ggcggcgggg gggcatgtgg cgcgggcgat cgcggcgacg ggagcggggg agtaggaagc
     3961 acgaaattcg aaatccgaaa ttcgtaataa agtggatcca aagccgtgtc ggcgcgttgc
     4021 gtggcgaagg tctggccgcg cgttc
//
LOCUS       DAOPCO010000338         4042 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18159_length_4042_cov_4.570855, whole genome shotgun sequence.
ACCESSION   DAOPCO010000338 DAOPCO010000000
VERSION     DAOPCO010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18159_length_4042_cov_4.570855"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /locus_tag="PLS90_15905"
     CDS             complement(<1..1852)
                     /locus_tag="PLS90_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03932.1"
                     /translation="MPDSDASADPKIRAFLRSAAGLLLILVVFVPLQLLRNAQEASLW
                     CDEIYSLMLAHHELEDLVDMTAVDDHPPGYYILLKTWNRIGRELEIAPGVFWSRLPGT
                     LGWITMLLFAWFILRGLLGPSGGTLACWALAASAQLLLHARNMRGYALALPALLIMFL
                     LLAWQFRLTRRGAGRPWNELPRAAATAGWIVYFLCGALALWCHLLASFVLLFLGFSWL
                     LLSGIIIARQGMRDKSAALFLWAGAHSHLFIILAFLPWLLFVPSNVAHKAGTLVEWAT
                     PPTLPNLLRVFLLWYPMGPVAGPHVAAEGLRLFQWLAALSALAPLSIVLYARFRPRGG
                     PRVDPSPSLFVPLAGVLIPFLFTLTIWLIDRLTAIPAFDGTRYAAMPVGIWAIGLAAA
                     VTLAARRLALPNRFAWLIMLPWFASSAVALAMEISIESRGGLANWIQLPESHLPPPGS
                     DLYATPSELIPYFADSLAAYNVHRIEEIANVPDGTDELAILRLNQWGGVERERDAVIL
                     AFVSAGKLAAAQQAYRFPVPSYTQFVTHRLEGFDPTAARALWQLGYNTAPRDFPADTL
                     LRMDAHDLAMRDGWNNLESDANRAALRWSRTELALAPLRGAEREFMGLFQR"
     gene            complement(1933..>4042)
                     /locus_tag="PLS90_15910"
     CDS             complement(1933..>4042)
                     /locus_tag="PLS90_15910"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03933.1"
                     /translation="GVGKTEVAKQLAYCLGISLIDPLQYKLVAKSLVSEGELFPPICI
                     EVPRSAVPSILQWLRETFGEDHLVEIGRVIDLRRDQMINELANWASMTDEERRLTHRE
                     KARLRSAGAAQRLDELAESTRSRRWRDPAFLGDIAARLAPRPRGWQGAGDRYALSGEP
                     LEWMVPRLRSTQGRVVSGLEEPAIDRLGLARLLFVPHGLLDILDKAMQAAHSQNPGLN
                     FAKIPLDDRPTYELLQRGDVSGIPPLENVTLRVMLRKHAPANLLQLLRVKTESGMVRP
                     GEERPHELSEELPDVLLSYQCAYLKANYPLAFYAAAIDAVIESHQNPSALIRETRRAG
                     FDVLPPDINLSQWDTTIHGHSIRLGLSAIRGFGERAWENVHAVRSGGNFTTLENFCER
                     VNTRIVNLRILRALIASGAMDALDPNRAAMDAVIARLQKRTRERESAADPLHRQATLF
                     ELEEWETEPDADEPVADVREWNAWEKLQRESEALGFFLSIDPVARFRIVLEHLAPIPI
                     ERINRRHLGKDIRITGLVCAVEERSPLLGREGDRLVDLEGLAVTLPQRIAEVSQSCLE
                     PGTEVFLTGRLNRRDGMLLFDADGIWRLCDMEDQATRVGRVRLGLAEENRRTLGMIAK
                     LVRDYPGNTALELADYPAPRGWTYRRLARRGVFFCSPLYQGLCKILTPNAVELFGQNG
                     EPLLARASDLEDTKDPDEVD"
BASE COUNT          723 a         1218 c         1502 g          599 t
ORIGIN      
        1 tgcgctgaaa caggcccata aactccctct ccgcgccgcg cagcggagcg agggccagtt
       61 cagtgcggct ccagcgcagc gccgcgcggt tggcgtcgga ttcgagattg ttccagccgt
      121 cgcgcatggc gagatcgtgg gcatccatcc ggagcagggt gtcggcgggg aaatccctgg
      181 gggcggtgtt gtagccgagt tgccacagcg cgcgggcggc cgttggatca aagccctcca
      241 gccggtgcgt cacgaactgg gtgtaactcg ggacggggaa ccgataggcc tgctgcgctg
      301 cggcgagctt gccggcgctg acgaaagcca gaatcacggc atcgcgttca cgctccacgc
      361 ccccccactg gttcagccgg aggatcgcca gctcgtcggt tccgtcgggg acattcgcga
      421 tctcttcgat ccggtgcacg ttgtaggccg ccaggctgtc ggcgaaatac gggatcagct
      481 ccgacggcgt ggcgtaaaga tcggacccgg ggggcgggag gtggctctcg gggagctgga
      541 tccagttggc gaggcccccg cgcgattcga tcgagatctc catcgccagc gcgacggcgg
      601 aggaggcgaa ccaggggagc atgatcagcc acgcgaaccg attgggcagc gcgagccgcc
      661 gcgcggcgag ggtgacggcg gcggccagcc cgatcgccca gatgccgacc ggcatggccg
      721 cgtagcgcgt tccgtcgaag gccgggatcg cggtcaggcg gtcgatcaac cagatcgtca
      781 gggtgaagag gaacgggatc aacaccccgg cgagcgggac gaagagggaa ggggagggat
      841 cgacgcgcgg gccgccgcgc gggcgaaatc gggcatacag aactatggac agcggcgcca
      901 gcgccgacag cgccgccagc cattggaaca gtcgcaatcc ctcggcggcg acatgtggac
      961 cggcaaccgg ccccatcgga taccacagca ggaacacgcg cagcagattc ggcagggtcg
     1021 ggggcgtcgc ccactcgacc agcgtcccgg ccttgtgggc cacattgctc ggcacgaaga
     1081 gcagccacgg caggaaggcc aggatgatga acagatgact gtgcgcgcca gcccagagga
     1141 atagggcggc ggatttgtcg cgcatcccct ggcgggcgat gatgatcccc gacaacagca
     1201 gccaggaaaa ccccaggaac aacaggacga acgacgcgag gagatggcac cagagggcca
     1261 acgcgccgca caggaagtag acaatccagc cggcggtcgc cgcggcccgg gggagttcat
     1321 tccatggccg gccggcgccg cgccgcgtca ggcggaactg ccacgccagc aacagaaaca
     1381 tgatcagaag cgcgggaagg gcgagggcgt agccgcgcat gttgcgcgcg tgaagcagca
     1441 gttgcgcgga agcggcgagc gcccagcagg ccagcgtgcc gccggagggc ccgagcaggc
     1501 cgcgcagaat gaaccaggcg aagagcagca ttgtgatcca gccgagcgtg ccgggcaggc
     1561 ggctccagaa aacgcccggc gcgatttcca gttcccggcc gatccggttc caggttttca
     1621 gcaggatgta gtagcccggg ggatgatcgt cgacggcggt catgtcgacc aggtcctcca
     1681 gctcgtggtg cgcgagcatg agcgaataga tctcgtcgca ccagaggctg gcctcctggg
     1741 cattcctgag aagctgaagg ggaacgaaga cgacaaggat caggagcagg ccggcggccg
     1801 agcgcagaaa ggcgcggatt ttcgggtcgg cggaggcgtc ggaatcaggc atcggcgact
     1861 ttccagggtg ggatcgcgga atgcaaggtg gtcactcggc acgcgagagc cagcgtgaag
     1921 gaggagctga cgctagtcga cttcatcggg atcctttgtg tcctccagat cggaggcgcg
     1981 tgcgagaagc ggctcgccgt tctggccgaa cagttcgacc gcattgggcg tcaggatttt
     2041 gcagagcccc tggtagagcg gggagcagaa gaagacgccg cggcgggcga ggcgccggta
     2101 ggtccagccg cgcggggcag ggtagtcggc cagttccagc gcggtgttgc cggggtagtc
     2161 gcgcaccagc ttggcgatca tcccgagcgt ccgccggttc tcctcggcca gtcccaggcg
     2221 gacgcggccg acgcgcgtgg cctgatcctc catgtcgcag aggcgccaga tcccgtcggc
     2281 atcgaacagc agcatgccgt cgcggcgatt gagccggccg gtcaaaaaga cttcggtacc
     2341 gggttcaagg caggattggg agacctcggc gatgcgctgg ggcagcgtga ccgcgagccc
     2401 ttcgaggtcg accagccggt cgccctcgcg gcccaggagg ggactgcgct cctcgacggc
     2461 gcagacaagg ccggtgatgc ggatgtcctt gcccaggtgg cggcggttga tcctttcgat
     2521 cgggatcggc gcgagatgtt ccaggacaat ccggaagcgt gcgaccgggt cgatggagag
     2581 gaagaagccg agggcctcgg actcgcgctg cagcttctcc caggcgttcc actcgcggac
     2641 gtcggccacc ggctcatcgg catcgggctc ggtctcccac tcttccaatt cgaaaagggt
     2701 ggcctggcgg tgaaggggat cggcggccga ttcgcgctcg cgggttcgct tttggaggcg
     2761 cgcgatgacg gcgtccatcg cagcgcgatt gggatcgagc gcgtccatcg cccccgaggc
     2821 gatgagcgcg cgcaggatac gcagattgac gatgcgcgtg ttgacgcgtt cgcagaagtt
     2881 ttccagcgtg gtgaaattgc cgccggagcg gacggcgtgg acgttttccc aggcgcgttc
     2941 gccgaagccg cggatggccg acaaccccag gcggatcgaa tggccgtgaa tcgtggtgtc
     3001 ccactggctg aggttgatgt cgggggggag gacgtcgaat ccagcgcggc gcgtctcgcg
     3061 gatcagcgcc gaggggttct ggtgcgattc gatgacggcg tcgatcgcgg cggcgtagaa
     3121 cgccaacggg tagttggcct tcaggtaggc gcactgatag gacaggagga cgtcggggag
     3181 ttcctcgctc agttcgtggg ggcgttcctc gcccggccgg accatgcccg attcggtctt
     3241 gacccgcagc aactggagca ggttggcggg ggcgtgcttg cgcagcatca cgcgcagggt
     3301 gacgttctcg agtggcggga tgccgctgac atcaccgcgc tggagcaact cgtaggtggg
     3361 ccggtcgtcg aggggaatct tggcgaagtt caggcccgga ttctgggaat gcgcggcctg
     3421 cattgccttg tcgaggatat cgagcaggcc gtgggggacg aacagcaggc gtgccagccc
     3481 gagccggtcg atcgccggct cctcgaggcc gctgacgacg cggccctggg tcgagcggag
     3541 gcgggggacc atccattcga gcggttcgcc gctgagggcg tagcgatcgc cggcgccctg
     3601 ccagccgcgg ggacgggggg cgagccgggc ggcgatgtcg ccgagaaacg cgggatcgcg
     3661 ccagcggcgc gagcgggtcg attcggccag ttcatcgagc cgctgggcgg cgccggccga
     3721 acgcaggcgg gccttttcgc gatgggtcag ccggcgttct tcgtcggtca tcgaggccca
     3781 gttggccagt tcattgatca tctgatcgcg gcgcaggtcg atgacgcggc cgatctcaac
     3841 caggtgatcc tcgccgaacg tctcgcgcag ccactgtaga atcgagggga cggcgctgcg
     3901 cggaacctcg atgcagatcg gcggaaagag ctcgccctcc gagaccaggc tcttggcgac
     3961 aagcttgtac tgcagcggat cgattagcga gatgcccagg caataggcca gctgcttggc
     4021 gacttcggtt ttgccgacgc cg
//
LOCUS       DAOPCO010000339         4030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18219_length_4030_cov_5.658113, whole genome shotgun sequence.
ACCESSION   DAOPCO010000339 DAOPCO010000000
VERSION     DAOPCO010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_4030_cov_5.658113"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..1203)
                     /locus_tag="PLS90_15915"
     CDS             complement(19..1203)
                     /locus_tag="PLS90_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPK03934.1"
                     /translation="MALNATVRGAMPTHGGKTGIAVAGYDPLVDDSPPTIPYEGGGRY
                     EISDLLRKAIEVGASDLHMAVGRPPILRLHGQLHNLTGEPLTPPETRRLIYGILNDQQ
                     KQKFEEEKELDFSLAVSNMGRFRVNVHLQRSTVAAAFRVIDSRIRSFQELNLPTRVME
                     YLSRRPNGFVLITGPTGSGKSTTLAAMVDLINTERDCHIITIEDPIEYLHHHKRALIE
                     QREVNEDTYSFQNALKYCLRQDPDVLMIGEMRDLETISAAITAAETGHLVFSTLHTVS
                     AVQTVDRMIDVFPPHQQEQIRIQLAAVIEGAICQKLLPSARGSREIALEIMIATDAIR
                     NLVREGNTHMIQGTIEAGGKWGMVTMDRSILDLYRQGAITKEVALLNCNKPVEMKRQM
                     GL"
     gene            complement(1368..3776)
                     /locus_tag="PLS90_15920"
     CDS             complement(1368..3776)
                     /locus_tag="PLS90_15920"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HPK03935.1"
                     /translation="MSFRLATPLPFGTRAEAAAPTDAPAPGSPFHLFWRRVTFFLVLL
                     GFLALLSTGELTFYAGGLFLVVWLVGYIHPGRPRWWTEWMSHMLIWASLGAIAFLAIQ
                     ARFDSLLYLLIFLGLYKCLTLREPLDHLHAMLMSFFMLLACSIISTSVAFVLFLLLFM
                     VLITLDLVCLSIGRGGAGGRLGGAPVDLRPALRVPGHFFQRMVLNSTLLGLTILLVAF
                     ALFLTMPHFLPRNLNSPWSRNDPTQGGSSSGYRDEVTFTPIDRIQLDDTQVITVAARW
                     ADRANTAPLPTTLRLRGEALDYFDERQWRRDAENAVYQSDTWGAVAMSGLNNRPAPIL
                     EQRIYQDLTMNRLFGAGAPVWFDFSALQDHGWRVDLTLNRSGQSLMMNNHPMFRRRAA
                     PRFNDNHMPYYVYSKIEEDHLALLRRLAGSSPGSASAQVRRPLQVYQDGRPTVLEQIE
                     ATELGLDPNLTLPTNERFRCTRLPDTPLIDKIRELSFNHATSGSRAGKVLQLVEWLRN
                     DFEYSLDLENTPRVHPLETFLTRSHKGHCEYFASSLALLLRAQAIPARVVSGFYTADF
                     QDRADGRRFIVRQSDAHAWTEVWLDGVGWLTIDPTPPLDRGRAAQRLLQVSRWERLYE
                     RARLLWQTYVLDYSSSQTGPLARMFSGGWITRLRNLVRPVGDDLASKFELRSRQGADS
                     DRLAGRLVRLIVPPAFLAALVLFLVLQGRLRRRQSTGAGQRSPVLFINQLIRRLQALG
                     WTRQPSQTLAEWFHEIDRTTRGQYELAWILDLYHRCRFGDERLGPRDEQRAQELIRAI
                     RRPG"
     gene            complement(3858..>4030)
                     /locus_tag="PLS90_15925"
     CDS             complement(3858..>4030)
                     /locus_tag="PLS90_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HPK03936.1"
                     /translation="RHRIVVEFLLSLGIPRDTALIDAEGIEHHVSQATLEAMRRFAEA
                     AVAADAPTPLSA"
BASE COUNT          688 a         1252 c         1475 g          615 t
ORIGIN      
        1 tacgccggcg gcggcgacct acagccccat ctggcgcttc atttcgaccg gcttgttgca
       61 gttgagcagc gcgacttcct tggtgatcgc cccctggcgg tacaggtcca ggattgaccg
      121 gtccatcgtg accatgcccc acttgccgcc ggcctcgatc gtcccctgga tcatgtgggt
      181 gttcccctcg cggaccagat tgcgaatcgc gtcggtggcg atcatgatct ccagcgcgat
      241 ctcgcggctg ccgcgcgccg aggggagcag cttctggcag atcgccccct cgatcaccgc
      301 cgccaactgg atgcggatct gctcctgctg gtgcggcggg aaaacgtcga tcatgcggtc
      361 gaccgtctgc accgcgctga cggtgtgcag cgtcgagaag accaggtggc ccgtttcggc
      421 ggccgtgatc gcggcgctga tcgtctccag gtcgcgcatc tcgccgatca tcagcacgtc
      481 ggggtcctgc cgcaggcagt acttcagcgc gttctggaac gagtaggtgt cttcgttgac
      541 ctcgcgctgt tcgatcagcg cccgcttgtg atggtgcaga tactcgatcg ggtcctcgat
      601 cgtgatgatg tggcagtcgc gttcggtgtt gatcaggtcg accatcgcgg cgagcgtcgt
      661 cgacttgccc gacccggtcg ggccggtgat cagcacgaag ccgttgggcc ggcgcgagag
      721 atactccatc acgcgcgtcg gcaggttgag ttcctggaag ctgcggatgc gcgagtcgat
      781 gacgcggaac gcggcggcca ccgtcgagcg ctgcagatgg acgttgacgc gaaagcggcc
      841 catgttcgag accgccagcg agaagtccag ttccttttcc tcttcgaact tctgcttctg
      901 ctggtcgttg aggatgccgt aaatcagccg gcgggtctcc ggcggcgtca gcggctcgcc
      961 ggtcaggttg tgcagctggc cgtggaggcg caggatgggc gggcggccga cggccatgtg
     1021 gaggtccgag gcgcccactt cgatcgcctt gcgcagcagg tcgctgatct cgtagcgccc
     1081 gccgccctcg tagggaatcg tcggcgggga atcgtcgacc aggggatcgt agccggcgac
     1141 ggcgatgccg gtcttgccgc catgggtcgg catggcgccg cggacggtgg cgttgagtgc
     1201 catggatgcc tccaactgtc aggaccgacg cgtgggggga cgcacactcc cccgccgggc
     1261 gacccgcaag ggtacggcac gacgtcgctg tctctggacc ctatcggcat cggcggggcg
     1321 aaagatgatc cggaaattga aagggaacaa ggcgggggac gcaggggtca gccgggacgc
     1381 cggatcgccc ggatcaattc ctgggcgcgc tgctcgtcgc gcggccccag tcgctcgtcg
     1441 ccgaatcggc agcggtgata cagatcgagg atccaggcca gctcatactg cccgcgcgtc
     1501 gtccggtcga tctcgtgaaa ccactcggcg agcgtctggc tcggctggcg ggtccacccc
     1561 agcgcttgca gccggcggat gagctggttg atgaagagga cgggagaccg ctggccggcg
     1621 ccggtgctct ggcgccgacg cagccgcccc tgcagaacga ggaacaggac cagcgcggcg
     1681 aggaacgccg gcggcacgat cagccgcacc aggcggccgg cgaggcggtc cgaatcggcc
     1741 ccctggcgcg agcgcagctc gaacttgctg gccaggtcgt cgcccaccgg gcggaccagg
     1801 ttacgcagcc gggtgatcca gccccccgaa aacatccgcg cgagcggacc ggtctgactg
     1861 ctcgaatagt ccagcacata ggtctgccac aggaggcgcg cccgttcgta gaggcgctcc
     1921 cagcggctga cttggagcag gcgctgcgcc gcgcggccgc gatccagcgg cggggtcggg
     1981 tcgatcgtca gccagccgac gccgtcgagc cagacttcgg tccaggcgtg cgcgtcgctc
     2041 tggcgcacga tgaaccggcg gccgtcggcg cgatcctgga aatccgccgt gtagaacccc
     2101 gagacgacgc gtgcggggat ggcttgggcc cgcagcagga gcgccagcga cgaggcgaaa
     2161 tactcgcagt gccccttgtg cgagcgggtc aggaaggtct ccagcgggtg gacgcgcggg
     2221 gtgttctcca ggtcgagcga atactcgaaa tcattgcgca gccactcgac cagttgcagg
     2281 accttgccgg cgcgcgagcc gctggtggcg tggttgaagg aaagctcgcg gatcttgtcg
     2341 atcagggggg tgtcgggcag gcgcgtgcag cgaaagcgct cgttggtcgg cagcgtcagg
     2401 ttcggatcca gccccagttc ggtcgcctcg atctgttcaa gcacggtggg gcggccatcc
     2461 tgatagacct ggaggggccg gcgcacctgg gcggacgccg agccgggcga cgagccggcc
     2521 agccggcgca gcagcgccag gtggtcctcc tcgatcttcg agtagacgta gtagggcatg
     2581 tgattgtcgt tgaaccgtgg cgccgcacgc cgccggaaca tcgggtggtt gttcatcatc
     2641 aacgactggc cggagcgatt gagcgtcagg tcgacgcgcc agccgtgatc ctgcagcgcg
     2701 gagaagtcga accagaccgg cgcgcccgcc ccgaacaggc ggttcatcgt caggtcctgg
     2761 taaatccgct gttcgaggat gggggcgggg cggttgttca ggccggacat cgcgacggcg
     2821 ccccaggtat cgctctggta gacggcgttt tcggcgtcgc ggcgccactg gcgctcgtca
     2881 aagtagtcca gggcctcgcc gcgcaggcgc agcgtcgtcg gcagcggcgc ggtattggcg
     2941 cggtcggccc agcgcgccgc gacggtgatc acctgcgtgt cgtccagttg gatccggtcg
     3001 atcggcgtga aggtcacttc gtcgcgatag cccgagctgg agcccccctg cgtcggatcg
     3061 ttgcgcgacc aggggctgtt cagattgcgc ggcaggaaat gcggcatggt caggaagagc
     3121 gcgaaggcga ccagcaggat cgtgagcccg agcagcgtac tgttcaggac catgcgctga
     3181 aagaagtggc cgggcacgcg cagcgccggc cggagatcga ccggcgcccc ccccaggcgc
     3241 ccgccggcgc ccccccggcc gatcgacagg cagaccaggt ccagcgtaat gagcaccatg
     3301 aacagaagca gaaagaggac aaacgcgacg gaggtcgaaa tgatcgagca ggccagcagc
     3361 atgaagaacg acatgagcat cgcgtgcaga tgatcgagcg gctcgcgcag cgtcaggcac
     3421 ttgtaaagcc cgaggaagat gagcaggtag agcaggctgt cgaagcgggc ctggatcgcg
     3481 aggaaggcga tcgcccccag cgaggcccag atgagcatgt gcgacatcca ctcggtccac
     3541 cagcgcgggc gacccgggtg aatgtagccg accagccaga cgacgaggaa caggccgccg
     3601 gcatagaaag tgagttcgcc ggtggagagg agcgcgagga agcccagcag gacgagaaag
     3661 aaggtgacgc gccgccagaa gagatgaaac ggggagccag gcgccggggc gtcggtgggc
     3721 gcggcggcct cggcgcgcgt cccgaacggc agtggcgtgg cgagtcgaaa cgacataaga
     3781 cagcgagtat aacgcaggga tggccgggcg ggcgcgggaa ttgcgttatg cgaggcggcc
     3841 gggccggcgg gtgagggtca ggcgctgagg ggggtcgggg cgtcggccgc gaccgccgcc
     3901 tcggcgaaac ggcgcatcgc ctcgagcgtc gcctgtgaga cgtggtgttc gatcccctcg
     3961 gcgtcgatca gcgcggtatc gcgggggatc ccgagcgaga ggaggaactc cacgacaatc
     4021 cggtggcgtc
//
LOCUS       DAOPCO010000340         3988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18445_length_3988_cov_4.171625, whole genome shotgun sequence.
ACCESSION   DAOPCO010000340 DAOPCO010000000
VERSION     DAOPCO010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18445_length_3988_cov_4.171625"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PLS90_15930"
     CDS             <1..896
                     /locus_tag="PLS90_15930"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HPK03937.1"
                     /translation="DGGGRFQESIRSFDGWYDRAVRNLREVRNRIDAHPERLQLVLRG
                     EDFEKARRDGRSAIVLGFEGGKPLEGSLEKLRDLFERGLRQLQFTWAFRNQLADAQDE
                     TEDNGLTPFGREVVREINRLGIVGDMTHISKRAFREVMELSDDPIIASHSSGFGFCPD
                     PGNLDDDQLRMIAQKGGVAGVHFASHVVKWPNTLATIEDVVDHACYMIDVAGIDHVGL
                     GVDFFPVDEPYRESQKVFLNEVDPRGRERLDLFTFVKGMDRFSRLRTLIPYLRLRGFH
                     DDEIRKLMGGNWLRVYRQILP"
     gene            944..3073
                     /locus_tag="PLS90_15935"
     CDS             944..3073
                     /locus_tag="PLS90_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03938.1"
                     /translation="MQPARFINGIVWPWRLIGFALLAGLCESAPETGFPGATFDSGIP
                     PAGEARGGEWATAEGAIRHAGAGETPGLLLLEDAIRSACTVEARVRLEASAVGAAAGL
                     VLQYTEPGNYALLTLEQRRGGPYAVLSMVTPDGKKTVGDQSWLTGTDLAQWHTLTVQM
                     NGSAMVASVDGQPAARFWFRGEPPAYNSHGRNWEQDPEAGRIGVYALGRAVFDDIRVG
                     PLEADAELVTPQRGRWDADGRLLAQQSYDETMDRTTDWLLRSDAVVDKSLAAESIRSL
                     PPYVLANFVLSNDSLYEPLGGEFAFNHAWQILGIVQYYNYTGDRDVLKVATEIADWHL
                     ENLTPDDWALPNLPPSVVTFEADGRWRGIDWGLELDKSAYFGLALLRLHAATGDARYR
                     QAALDIGETLRPHQRDEGNWPFRINAQTGEVQDGYTCSQLWYVWFFERLARETGDPED
                     QARSDRALAWLLEHPVRDNQWIGLYGDIESGRKSLDQWVAQETAMHLLERRNEIPGAV
                     ETARGILDWVERTLVVDYGLHPGVPGVLEQTEYRVVLTHHQLRLAELYATLYAATGEE
                     ALRRKAIETANSVTWCVMSDGKMRQGFWYHAAGVPLQVGFNMQFCRIMAAVPETAPRD
                     ENHLLQWTADLRRIEYGADGIRYETIGPGEETFVVARPPRAVRAEGEPLAQRERWGFG
                     LNGWRYEPETGRLRLRHEAPSVEITFE"
     gene            complement(3191..3715)
                     /locus_tag="PLS90_15940"
     CDS             complement(3191..3715)
                     /locus_tag="PLS90_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HPK03939.1"
                     /translation="MGSEPREVRMMAGADGDKAFRDQLCQMLRGGMAFTSVADALADF
                     PTDKRGISAKGVPYTPWQLLEHMRIAQWDILEFSRNPRHESPDWPDGYWPDDRAPAAA
                     DAWDRSVRTLLDDLKALQDLVRDPHNDLHAPLPHGDGQTLAREALLAAHHTAYHLGQF
                     ILLRRLLGCWPPRK"
BASE COUNT          639 a         1165 c         1521 g          663 t
ORIGIN      
        1 aagacggcgg cggccggttc caggaatcga tccgctcgtt cgacggctgg tatgaccgcg
       61 cggtccgcaa tctgcgcgag gtgcggaatc ggatcgatgc tcatccggag cggctgcaac
      121 tggtgctgcg aggggaggat ttcgagaagg cgcggcgcga cggccgctcg gcgatcgtgc
      181 tgggcttcga agggggcaag ccgctcgagg ggagcctcga gaagctgcgc gatctgttcg
      241 agcgggggct gcgccagctg cagttcacgt gggcgttccg caaccagttg gccgatgcgc
      301 aggacgagac cgaggacaac gggctgacgc cgttcgggcg ggaggtggtg cgcgagatca
      361 accggctggg gatcgtcggc gacatgacgc atatttcgaa gcgggcgttt cgcgaagtga
      421 tggaattgtc ggacgatccg atcatcgcct ctcactcgag cggattcggc ttctgccccg
      481 acccgggcaa cctcgatgac gaccaactgc ggatgatcgc gcagaagggg ggggtggcgg
      541 gggtccactt cgcctcgcac gtggtaaaat ggcccaacac gctggccacg atcgaggatg
      601 tcgtcgatca tgcctgttat atgatcgatg ttgccgggat cgatcacgtc gggctggggg
      661 tggacttttt ccccgttgat gaaccgtacc gggaatcgca gaaggttttt ctgaatgagg
      721 tcgatccgcg ggggagggag cggctcgatc tgttcacgtt cgtcaagggg atggaccggt
      781 tcagccgact gcggaccctg attccgtacc tccgcctgcg cggttttcat gacgacgaaa
      841 tccgcaaact gatgggcggg aactggctgc gcgtctatcg ccagatcttg ccctgaactt
      901 cgctcaaccc cacagcacat cgcgatacga caggaggtga acggtgcagc cggcgcgatt
      961 catcaatgga atcgtttggc cgtggcgtct gattggattc gcgctgctgg ccggcctctg
     1021 cgagtcagcc cccgagaccg gctttcccgg cgcaacgttt gactcaggca ttcccccggc
     1081 cggcgaggca cgcggcgggg agtgggcgac ggcggagggg gcgatccggc acgcgggcgc
     1141 gggggagacg ccggggctgc tgctgctgga ggatgcgatc cgttcggcgt gcacggtgga
     1201 ggcgcgggtg cggctggagg cgagcgccgt gggcgcggcc gccgggctgg tgctgcaata
     1261 tacggagccg ggcaattatg cgctgctgac gctggagcag cgccgcgggg gaccgtatgc
     1321 cgtgctgtcg atggtcacgc ccgacgggaa gaagacggtc ggcgaccagt cgtggctcac
     1381 ggggacggac ctggcgcagt ggcacacgct cacggtgcag atgaacggca gcgcgatggt
     1441 ggcgagcgtc gacgggcagc cggcggcgcg cttctggttt cgcggtgagc cgccggccta
     1501 caactcgcac gggcggaact gggagcagga tcccgaggcg gggcgcatcg gggtgtacgc
     1561 cctcggccgc gccgtgttcg atgacatccg cgtcggcccg ctggaggcgg atgcggagct
     1621 ggtcacgccg cagcgcggcc gctgggacgc cgacggccgg ctgctggcgc agcagtcgta
     1681 tgatgagacg atggatcgga cgaccgactg gctgctgcgc agcgatgcgg tggtggacaa
     1741 gtcgctggcg gctgaatcga tccggtcgct cccgccgtac gtgctggcga acttcgtgct
     1801 gtcgaacgac tcgctctatg agccgctggg gggcgagttc gcgttcaacc acgcctggca
     1861 gatcctgggg attgtgcaat attacaatta cacgggcgat cgcgacgtgc tgaaggtggc
     1921 gaccgagatt gcggactggc atctggagaa tctgacgccg gatgactggg cgttgccgaa
     1981 cctgccgccc agcgtcgtga cgttcgaagc cgacggccga tggcggggga tcgactgggg
     2041 tcttgagctc gacaagtcgg cgtatttcgg gctggcgctg ctgcgcctgc acgccgcgac
     2101 cggcgacgcg cgctaccgcc aggcggcgtt ggacatcggg gagacgctgc gcccgcatca
     2161 gcgcgacgag ggcaactggc cgttccggat caatgcccag accggcgaag tgcaggacgg
     2221 ctacacgtgc agtcaactgt ggtatgtctg gttctttgag cggctggcgc gggaaacggg
     2281 cgacccggaa gaccaggcgc gcagcgaccg cgcgctggcc tggctgttgg agcatccggt
     2341 gcgcgacaac cagtggatcg ggctgtatgg cgacatcgag agcgggcgca agtcgctgga
     2401 tcagtgggtg gcgcaggaaa cggcgatgca cctgctcgag cggcgcaacg agattcccgg
     2461 ggcggtggaa acggcgcgcg ggattctgga ctgggtcgag cggacgctgg tcgtcgatta
     2521 cgggctgcat cccggcgtgc ccggcgtgct ggagcagacc gaatatcggg tcgtgctgac
     2581 gcaccaccaa ctgcgtctgg ccgaactgta tgcgacgctc tacgcggcga cgggcgagga
     2641 agcgctccgg cgcaaggcga tcgagacggc caattccgtg acctggtgcg tgatgtcgga
     2701 cggcaagatg cgccaggggt tctggtatca cgccgccggg gtgcccctgc aggtcgggtt
     2761 caacatgcag ttctgccgga tcatggccgc cgtgccggag acggcgccgc gcgacgaaaa
     2821 ccatctgctg caatggaccg cggatctccg gcggatcgag tatggggccg acgggattcg
     2881 ctacgaaaca atcgggccgg gcgaggagac cttcgtggtg gcgcggccgc cgcgcgcggt
     2941 gcgggcggag ggcgaaccgc tggcgcaacg cgagcgctgg ggattcgggc tgaacggctg
     3001 gcggtatgag ccggagaccg ggcggctgcg gctgcggcac gaagccccat cggtggagat
     3061 tacgtttgag tgagcgttga acaccggttg tggcgtcggg ctgaaagaac gaaaactggt
     3121 cgcttgccct ggtttcgctg agagtcgagt ttttgaatga gccggcgggc cgggcggcgg
     3181 cgcggggcgt tcatttgcgc ggcggccagc agccgagcag ccggcgcaac aggatgaact
     3241 ggcccagatg ataggctgtg tgatgggcgg cgagcagcgc ctcgcgggcg agcgtctggc
     3301 cgtcgccatg ggggagcggg gcgtgcagat cgttgtgcgg gtcgcgcacg aggtcctgca
     3361 gcgccttgag gtcgtcgagc agcgtgcgga cgctgcggtc ccacgcgtcg gcggcggccg
     3421 gggcgcggtc gtccggccaa tacccgtcgg gccagtcggg cgattcatgg cgcgggttgc
     3481 ggctgaactc gaggatgtcc cactgggcga tgcgcatgtg ttcgagcagt tgccagggcg
     3541 tgtaggggac tcccttggcg ctgatgccgc gtttgtcggt ggggaagtcg gccagcgcgt
     3601 cggccaccga ggtgaaggcc atgccgccgc gcagcatctg gcagagctga tcccgaaacg
     3661 ccttgtcgcc gtcggcaccg gccatcatgc gcacctcccg gggttcgctt cccatcgtgc
     3721 gcctccggcg gtttcaattc aatgcctacc cgatcagatg tccccttggt ccctcgcggg
     3781 tcgcgcacga ccgagagctt gatccgggag atcgaatctt gccacggctt ctccgcgacg
     3841 ccgccaggct gatccgggcg tacacacggg tgggagggag cgggcgccgg cgtgagcgcc
     3901 gggcgccggc gcgcttcgga tcaggccggc tcacgacgtg gggcacatag tcggcgagaa
     3961 tgacgatctc ctggtacggg tcgtcgtc
//
LOCUS       DAOPCO010000341         3986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18458_length_3986_cov_4.721699, whole genome shotgun sequence.
ACCESSION   DAOPCO010000341 DAOPCO010000000
VERSION     DAOPCO010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18458_length_3986_cov_4.721699"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLS90_15945"
     CDS             complement(<1..1318)
                     /locus_tag="PLS90_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03940.1"
                     /translation="MSGFILWVLIAWTASGAATFEPWHAIFQVVGPIASEKAPGRWRP
                     WFEPRYEPLPLDGSATIPDELVVDGIRETPRSFDYEGQELDFEQLWHTTSGRGIVYFF
                     KEEAVPAAGLLQVQCRAVGWVKWWVNGRLAYSWGGDGDQWQDHLVQLPLDKGENVICM
                     RLAAGAGGWKLQMNRSPSVQTTPIGFEGGPIQIQMEDIEQVWSPVGFDEFAQFEKGLR
                     AKAPSHRSATAVLGEESDDPDRWVRLRIKPLIVDGFSTDFFIQIKDGCELGFTSNYDA
                     DEQKQQVIWQIRVDGRSVWEVDHYVVKDWAGISPLADARQISQTRESKALDLDSSLGD
                     RVGETNEIELIIASRGDELTLRTNHGAEDIGYRATGKAMEGLVLRKKIDGVGDRMGKV
                     GLSLRGLDIKIENFNAQPLPDSIKQTWALCDNPPIRLGVVPRDPRRP"
     gene            complement(1484..3295)
                     /locus_tag="PLS90_15950"
     CDS             complement(1484..3295)
                     /locus_tag="PLS90_15950"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK03941.1"
                     /translation="MVMNPIRLFTALALLSLAAFIPDGSVHAEVIDDFDDGAWNAGDN
                     WDAENIVQGRYPDTIEFDESLVPGMLAIRYTDTGAGDQATLILRDDQKLNVGQWVRVD
                     AAINSGNSRGEIRIGLGLAASRGRNAPAQQGRDRANILFWGLRSDRVVRAHAYGAEGE
                     EVGNAQAPLRNYAAGSFVTLAIRRVQPRQFELYFAPTGEPLEFVEAITFPDTEGSIPP
                     DIPGLMIDPGNSSHIVLVDEFEIGDGWPPPSRPARPASALLPLPAPEVPENQLALTLP
                     NNILDLPKLPGTRHVIYKPKGDGDNTYHHNAMIVRWRGRFYVAWHTSPKSEATSPFTG
                     MLATSVDGEQWSPPVSFDIIPRGWNPTEDRLYMWGDGRVFYTEDGEQWNELPAERLAN
                     VPKYILLTASNRSFGHLRDGRLMAYGLGPGAPQFPITTDPSGLSGWTLNLIDKGECWD
                     VGEPGGYEGPDGVLHGTIRCGTHVWHTYSRDGGETWDTLRRQTHFTDCPGNKQFGVFP
                     DKSVWYIGNPVPGSRMELVLGHSRDGWIFDDNYLVRWEPIEPIWPSDHKSGSSPGYEY
                     PGGLYHDGKMYVVYSRTRDYMEVSIVDVSGIVGELTE"
     gene            complement(3327..>3986)
                     /locus_tag="PLS90_15955"
     CDS             complement(3327..>3986)
                     /locus_tag="PLS90_15955"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK03942.1"
                     /translation="PDYSFSSSNKHYVRLRDGRLMQAELGRAQDPALSAKGLWAVSWL
                     THDPTGLTGWSGGYVDASACPDPGEPAGYEGPDGVLHYIARCGPRIFHAYSEDGGQTW
                     TQLVQQPQFTDSPSNKEFGTLPDGSVWCIANPIPGNRQHLVLGISRDGWNFNETYLVR
                     WEPHTPRFPAPHKLERPGYEYPGAWYHDGKLYAAYSIWREGIGVSIIDLAGEGLIKTD
                     Q"
BASE COUNT          799 a         1286 c         1077 g          824 t
ORIGIN      
        1 ccggacggcg cggatcacgt ggaacgaccc ctaaccgaat aggcggattg tcacaaagag
       61 cccacgtctg cttgatcgaa tcggggagag gttgagcgtt gaagttttca atcttgatat
      121 ccaaccccct taaactcaat ccgacctttc ccatccgatc acccacgccg tcaattttct
      181 tgcgcagaac cagcccttcc atcgccttgc ctgtcgcccg ataaccgata tcctcggctc
      241 catgattagt gcgtagcgtc aactcatcgc cgcgcgaggc aataatcagt tcgatttcgt
      301 tggtttctcc cacacggtcg cccagactgc tgtccagatc aagggctttg ctttcgcgcg
      361 tctggcttat ctgacgcgca tcggccagcg gagaaattcc cgcccaatcc ttgacaacgt
      421 aatgatcgac ctcccaaacc gaccgcccat cgactcgaat ctgccagatc acctgctgct
      481 tttgttcatc tgcgtcataa ttacttgtaa agcccagttc gcagccgtct tttatttgga
      541 tgaagaagtc ggtggaaaat ccgtcaacga tcaacggctt gatgcgcagc cgaacccaac
      601 gatccgggtc gtcactttcc tctcccagaa ctgctgtcgc actgcgatgc gagggcgcct
      661 tcgccctgag tcccttttcg aactgagcga attcatcaaa gcccaccggc gaccacacct
      721 gttcaatgtc ttccatttga atctggatcg gaccaccctc aaagccgatg ggtgtcgttt
      781 gaacgctggg ggaacggttc atttgcagtt tccagcctcc cgctcccgcc gccagacgca
      841 tgcaaatgac attttctcct ttatcgagcg gcaattgaac gagatgatcc tgccattgat
      901 cgccgtctcc gccccaggag taagccagtc ggccattgac ccaccatttc acccacccca
      961 cggcgcggca ttgcacctga agcaaccccg cggcaggcac cgcttcctcc ttgaaaaaat
     1021 agacaatgcc ccgtcccgac gtggtgtgcc atagttgttc gaaatccaac tcctgtcctt
     1081 cataatcgaa ggaacgcggt gtctctcgga tcccgtccac aacgagttcg tcgggaatcg
     1141 tcgcgctccc gtccaagggg aggggctcat accgcggctc aaaccagggc cgccaacggc
     1201 ccggcgcttt ttcgctggca atcgggccga ctacctgaaa aattgcatgc catggctcga
     1261 acgtcgctgc accgcttgcg gtccatgcga tcaggaccca aaggatgaac cccgacacaa
     1321 ttctgaatct catttcaagc cctcgaaccg gtctcttgaa ttccaaaatg ttcagaattc
     1381 aagccgatga tatccagcgt gaacactgtc caaccttcag agcgcgagcc tacggtcaac
     1441 cgcacattga ttgcctgctt ttccagtcaa ggcaaatcac cattcactcg gtcaattcgc
     1501 cgacgatccc tgagacatcc acaatcgaga cttccatgta atcgcgcgtg cggctgtaaa
     1561 caacatacat cttgccgtca tgatatagcc cgcccggata ttcataaccg ggcgaactgc
     1621 ccgacttgtg gtccgaaggc cagatcggct cgatcggttc ccagcgcacc aggtagttat
     1681 catcgaaaat ccagccgtcc cggctgtggc ccagcaccag ttccatccga ctgcccggca
     1741 cgggattgcc gatgtaccac accgacttgt cggggaagac gccgaactgc ttgttgcccg
     1801 gacagtcggt aaaatgggtt tgtcgccgca gcgtgtccca ggtctcgcca ccgtcgcggc
     1861 tgtaggtgtg ccagacatgg gtgccgcagc gaatcgttcc gtgcagcacg ccgtcgggcc
     1921 cctcgtaacc gcccggttct ccgacatccc agcactcgcc tttgtcgatg agattaagcg
     1981 tccagccgct cagtccgctc ggatcggtgg taatcgggaa ctgcggcgcg cccgggccca
     2041 gcccgtaggc catcagtctc ccgtctcgca agtgaccgaa actccggttg ctcgcggtca
     2101 gcagaatata ttttggaacg ttcgccaacc gctccgccgg cagttcgttc cactgttcgc
     2161 cgtcctccgt atagaacacc cgtccgtctc cccacatata aagccggtct tccgtcggat
     2221 tccagccgcg cggtatgatg tcgaacgaga cgggcggact ccactgttcg ccgtcaaccg
     2281 aagtcgcaag cattcccgtg aagggcgacg tcgcctcgct tttgggcgaa gtatgccacg
     2341 cgacgtaaaa cctcccgcgc cagcgcacga tcatcgcgtt gtgatggtag gtgttgtccc
     2401 cgtcgccctt gggcttgtaa attacgtgac gcgtgcccgg cagcttgggc agatccagaa
     2461 tgttattggg gagcgtcagg gcaagttggt tctcgggcac ttcgggcgcg ggcagcggga
     2521 gcagcgcgga agccggccgg gccgggcggc tcggcggcgg ccagccgtcg ccgatctcaa
     2581 attcatcaac cagtacgata tgcgaggagt tccctgggtc tatcatcaaa cccggaatat
     2641 ccggcggaat cgacccttcg gtatccggaa acgtgattgc ctcaacaaat tcgagcggct
     2701 cgcccgtggg cgcaaagtag agctcgaatt gccgcggctg aacacgccga atcgcgagcg
     2761 tgacgaatga tcccgccgca taattgcgca agggcgcctg agcgttgccg acctcttcgc
     2821 cctccgcccc ataggcatgc gcccgcacca cgcgatcgct gcgcaacccc cagaacagga
     2881 tgttggcgcg atcccgcccc tgctgcgcgg gggcgttgcg cccccggctc gcggccaatc
     2941 cgagcccgat ccggatttca ccccttgagt tgccactgtt gatcgccgca tcgacccgca
     3001 cccactgccc gacattcagc ttctgatcgt cgcgcagaat cagcgtcgcc tggtcaccgg
     3061 cgcccgtgtc ggtatagcgt atggcgagca tgcccggaac cagcgattcg tcgaactcaa
     3121 tcgtatccgg ataacggccc tgaacgatat tttcagcgtc ccagttgtcg ccggcattcc
     3181 acgcaccatc gtcgaaatcg tctatgactt cagcatgaac ggaaccgtcc gggataaatg
     3241 ctgcaaggct caacagcgcg agggctgtga acagccgaat cggattcatc accatgacca
     3301 gcaatccttt catcaagaaa tgacttttat tgatcggtct tgataagtcc ctcgcccgca
     3361 aggtcgatga tgcttacccc gatgccctcg cgccagatgc tgtaggcggc atagagtttg
     3421 ccgtcgtgat accaggcccc cgggtattcg tagccgggcc gttccagttt gtgcggcgcg
     3481 ggaaagcgcg gcgtgtgcgg ctcccagcgc acgaggtacg tttcgttgaa gttccaccca
     3541 tcccggctga tccccagcac caggtgctgc cggttgcccg gaatcggatt cgcaatacac
     3601 cagaccgaac cgtcggggag cgttccgaat tccttgttgg atggagaatc ggtgaattgc
     3661 ggctgctgga ccagttgcgt ccaggtttgg ccgccgtcct cgctgtaagc atggaaaatc
     3721 cggggaccgc agcgcgcaat gtagtgcaga acgccgtcgg ggccctcgta gccggcgggc
     3781 tcgcctggat ccggacaggc gctggcgtcc acgtatccgc cactccagcc cgtcaatccc
     3841 gtcggatcgt gcgtgagcca ggagacggcc cagagcccct tcgcgctgag tgcgggatct
     3901 tgcgcgcggc cgagttcggc ctgcatcagg cgcccgtctc ggagccgtac gtagtgcttg
     3961 ttggacgagg aaaagctgta atcagg
//
LOCUS       DAOPCO010000342         3976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18517_length_3976_cov_5.442489, whole genome shotgun sequence.
ACCESSION   DAOPCO010000342 DAOPCO010000000
VERSION     DAOPCO010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18517_length_3976_cov_5.442489"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="nuoF"
                     /locus_tag="PLS90_15960"
     CDS             <1..1188
                     /gene="nuoF"
                     /locus_tag="PLS90_15960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPK03943.1"
                     /translation="DLRPLADDVLALVKEANIRGRGGAGFPAGVKWGFIPRNRNLPHY
                     VCVNADESEPGTFSNRPILEQRPHLLLEGAALAAYATLAETVYIYIRKEYYEGVRQTQ
                     RAIDDAYAAGLLGRNILGTGFNLDIHVHTGAGAYICGEETALIESLEGKRGYPRLRPP
                     FPAVQGLYNCPTVINNVETLAIVAEVARRGGDWFKSLGTEKSPGMTVYSISGHVRKPG
                     NYELPLGVTMRELIYEHAGGIRDGNKFKAVFPGGSSVPMLFEDELDVVMDFDGPRQYG
                     TFLGSTGIIVMDETVCLVWAALNLMHFYWDESCGQCTPCREGTGWMHKILKRLEHGQG
                     VQHDVDLLLDICKNIVGRSICALGEFSTGSLVRTIPRFRDEFQAHVDQQGCPFEKKYT
                     EFR"
     gene            1443..1895
                     /gene="tnpA"
                     /locus_tag="PLS90_15965"
     CDS             1443..1895
                     /gene="tnpA"
                     /locus_tag="PLS90_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPK03944.1"
                     /translation="MAHTYGSCLYHFVFSTRGRIDAIKSEWIFDLWEYMGGIARNSKM
                     SAICIGGIENHVHALIMIPPSVAPAKGIQLIKANSSKWVNETLRPKIPFGWQEGYGVF
                     TIGFSQIAKTREYIMTQAEHHRHTSFEEEYRAFLRKHMIAFEEDYIFD"
     gene            2192..>3976
                     /locus_tag="PLS90_15970"
     CDS             2192..>3976
                     /locus_tag="PLS90_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPK03945.1"
                     /translation="MTPLNVPPPPDNVTVTVNGAPMQLPKGKNLLQALLDVGHYIPHY
                     CYHPRLSVAGNCRLCLIELEGRPKPEIACNMVVADGLKIRTDSELVDSCRKGMMEFLL
                     INHPLDCPICDRGGECMLQRYSVEYGWGTARTIDPRRRFHKPQFDPLIDIERNRCIMC
                     TRCVRFCDEIAGEHVMGVFGHGNRNYIGTYGNGPVSNPFSGNVIDICPVGCLTSKPFR
                     FKARVWELRQTNTTTRTCNGAVTAWTRAGKLYRVTPPTRRRHGAYTIDEDTDEFISNE
                     ARFGTHYANHDDRLTTPMIAAGDGAQMTTSWKSALEAAARALRNAGRGEACFLAGERS
                     TNEEFYLLGRLARGLCRAGHIDWRMRFTSEAAARAAGDALRASDGDLDLLARGAYGAA
                     LMIHADLLGTAPDIALRIKEAARAGKTRLALLDARVDEFFGRFADPVYLEQPEALATA
                     VNNLAAALEHGRDEAHAPADGAPLFNHLARHEQGLIVLGLDAAGGALNPALVPAALRL
                     VQALGAGWQFLAVTAARNARGAVAAGAQSDRLPTGPVTDPEALRSTSELYGAPLEAPE
                     ETPAAPELLRRAAAGQFHAEGGLRAVVEQ"
BASE COUNT          726 a         1286 c         1305 g          659 t
ORIGIN      
        1 gacctgcgtc cgctggccga cgacgtgctg gcgctggtga aggaggccaa tatccgcggg
       61 cgcggcggcg cgggctttcc cgccggcgtc aaatggggtt tcatcccgcg caaccgcaat
      121 ctgccgcact acgtttgcgt caacgccgac gaaagcgagc cgggaacgtt cagcaaccgc
      181 ccgatcctcg aacagcggcc gcatctgctg ctcgaggggg ccgcgctggc ggcctatgcc
      241 acgctcgccg agacggtcta catctacatc cgcaaggagt attacgaggg ggtgcgccag
      301 acgcagcggg cgatcgacga cgcctacgcc gccgggctgc tgggtcggaa cattctggga
      361 accggcttca acctcgatat tcatgttcac accggcgcgg gcgcctacat ctgcggcgag
      421 gagacggcgc tgatcgaatc gctggagggc aagcgcggct atccgcggct gcgcccgccg
      481 tttcccgccg tccagggact gtataactgc cccaccgtca tcaataacgt ggagacgctg
      541 gcgattgttg ccgaggtggc ccgccgcggc ggcgactggt tcaagtcgct ggggacggaa
      601 aaatcgccgg gcatgaccgt ctattcgatc tcgggccacg tgcgcaagcc gggcaattac
      661 gagctgccgc tcggcgtcac catgcgcgag ctgatctacg agcacgccgg cggcatccgc
      721 gacggaaaca agttcaaggc cgtgttcccc ggcggcagtt cggtgccgat gctcttcgag
      781 gacgaactgg atgtcgtgat ggacttcgac ggcccgcgcc agtacggcac ctttctcggc
      841 tcgacgggca tcatcgtgat ggatgaaacg gtttgcctcg tctgggccgc gctcaatctg
      901 atgcacttct actgggacga atcgtgcggc cagtgcaccc cctgccgcga ggggaccggc
      961 tggatgcaca agatcctgaa gcggctggaa cacggccagg gcgtgcagca cgatgtcgac
     1021 ctgctgctgg atatctgcaa gaacatcgtc ggccgctcga tctgcgcgct gggtgaattc
     1081 tcgaccggca gcctggtgcg tacgatcccg cgcttccgcg acgaattcca ggcccacgtc
     1141 gatcagcagg gctgcccgtt cgagaagaaa tacacggagt tcagatagga ggtcgtgccg
     1201 cggactgaac catggaagtt gaagccctgc cgctttcatc tcgcgtatta cccaggcaat
     1261 ttcggggcgg ccatcgcgga cttcgccgtc ggtttccggt caacccggta gctggggagg
     1321 gcgaaaacag agtgagtccc gtcagggaca gcagatttca gcccgccacg tcagtggcgg
     1381 gtggcaaggg gcaggtgaga aagtcccgtg gggacggcag acgctgtgtc cggagggaac
     1441 cgatggctca tacatacggc agttgcctct atcacttcgt cttctcaaca cggggccgga
     1501 tcgacgcaat caaatccgaa tggatttttg acttgtggga gtacatggga ggaatcgcaa
     1561 ggaactcaaa aatgtcagca atttgtatcg gtgggattga gaatcatgtt cacgcactca
     1621 tcatgattcc gccctcggtc gctccggcca aaggaattca gctcatcaag gcaaattcct
     1681 ccaaatgggt gaatgaaacg ctaagaccga agatcccttt tggctggcag gaggggtatg
     1741 gcgtttttac catcggtttc tcgcagatcg cgaagacgcg cgagtatatc atgacgcagg
     1801 ctgagcatca tcgacacaca tcattcgaag aggaataccg tgcattcttg aggaagcaca
     1861 tgatcgcttt cgaggaggat tacatctttg actgacgaga cgacaatctc tgtcgtcccg
     1921 atgggactcc gtaactttat tgtcttatac ccgccactga cgtggcgggc tgaagtctgc
     1981 cgtccctgag ggacttaagt cgcgtcccgt caccgacatg gcgggcctga aacgtgtgcc
     2041 attcctgagg gagttggggc cgcccgtcaa agtaccgccc actaagactt gagaatgcgc
     2101 cgccggacgt ggaaaatgtt gcgtccctgt tgcggcggtc aggacagacg acgagcctgt
     2161 caactcggat cggaaccgga atagaagctt catgacccca ttgaacgtcc ccccgccccc
     2221 cgacaacgtg accgtcacgg tcaacggcgc cccgatgcag ttacccaagg gcaagaacct
     2281 gctgcaggcg ctgctcgacg tcgggcatta catcccgcat tactgctacc atccgcggct
     2341 gagcgtggcg ggcaactgcc gcctctgcct gatcgaactg gaggggcggc ccaagccgga
     2401 gatcgcctgc aacatggtcg tggccgacgg gctgaagatc cgcaccgaca gcgagctggt
     2461 cgacagttgc cgcaagggga tgatggagtt cctgctgatc aatcatccgc tcgattgccc
     2521 gatctgcgat cgcggcggcg agtgcatgct gcagcgctac agcgtggaat acggctgggg
     2581 cacggcgcgc acgatcgatc cccgccgccg gttccacaag ccgcagttcg acccgctgat
     2641 cgacatcgaa cgcaaccgct gcatcatgtg cacgcgctgt gtccgcttct gcgacgagat
     2701 cgccggcgag cacgtgatgg gcgtctttgg ccacggcaac cgcaactaca tcggcaccta
     2761 cggcaacgga ccggtcagca acccgttttc gggcaacgtg atcgacatct gccctgtcgg
     2821 ctgcctgacg tccaagccgt tccggttcaa ggcgcgggtc tgggaactgc gtcagacgaa
     2881 cacgacgacg cgcacctgca acggcgcggt gaccgcctgg acgcgcgccg gcaaactcta
     2941 ccgcgttacg ccgccgaccc gccgccgcca cggcgcctac acgatcgacg aggacaccga
     3001 cgagttcatc agcaacgagg cgcgcttcgg cacgcactac gccaaccatg acgaccggct
     3061 gacgaccccg atgatcgccg ccggcgacgg cgcccagatg acgacctcgt ggaagagcgc
     3121 gctggaggcg gcggcccgcg cgctgcgtaa cgccggccgg ggcgaggcgt gctttctggc
     3181 gggcgagcgt tcgaccaacg aggagttcta cctgctgggc cgcctggcgc gcgggctctg
     3241 ccgcgccggc cacatcgact ggcggatgcg tttcacgtcc gaggcggcgg cgcgcgcggc
     3301 gggcgacgcc ctgcgcgcca gcgacggcga cctggacctg ctggcgcgcg gcgcctacgg
     3361 cgcggcgctg atgatccatg ccgatctgct cggcaccgcc cccgacatcg cgctgcgcat
     3421 caaggaggcc gcgcgggcgg gcaagacgcg cctggcgctg ctcgatgccc gcgtcgatga
     3481 gttcttcggc cggttcgccg acccggtcta cctggagcag cccgaagcgc tggcgacagc
     3541 cgtcaacaac ctggccgcag ccctggaaca cggacgggac gaagcccatg cgcccgccga
     3601 cggcgcgccc cttttcaacc atctggcgcg gcacgagcag ggcctgatcg tgctggggct
     3661 cgacgcggcg ggcggcgcgc tgaaccccgc gctcgtgccg gcggcgctgc ggctggtcca
     3721 ggcgctgggc gcgggttggc agtttctggc cgtcaccgcg gcgcgcaacg cccggggggc
     3781 cgtcgccgcc ggggcgcaga gcgaccggct gcccaccggc ccggtcaccg atcccgaggc
     3841 gctgcgctcg acctcggaac tctacggggc gccgctggag gcgccggagg aaacgccggc
     3901 cgcccccgag ctgttgcggc gggccgccgc cggccagttc cacgcggaag gtggactgcg
     3961 cgcggtggtc gagcag
//
LOCUS       DAOPCO010000343         3959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18612_length_3959_cov_3.928279, whole genome shotgun sequence.
ACCESSION   DAOPCO010000343 DAOPCO010000000
VERSION     DAOPCO010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18612_length_3959_cov_3.928279"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1320
                     /locus_tag="PLS90_15975"
     CDS             367..1320
                     /locus_tag="PLS90_15975"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03946.1"
                     /translation="MRFPAVLLSMSLLLASLAPLTGCVWATQMRLHGQLNQAERAFEQ
                     GNYPLAATRYESLSNRYPDGPMREKILMRQGIAYYTVQAYHEARDTFLRYLHEYPQGH
                     YAADANEYIAKVDVLLSPGNPAEEAALAAALQDLNALQKLRMEHPTDPAVATAIGDLY
                     RDLGDYDEAMRYYYEAQELGATFQERDLLQARLMLDANGAPVPMTPEQVRLRNLEDNP
                     LVIYDVYDYKARDPGENGFNNRLQDYVVTGRIRNQSSRIIHDVELEVRFLNAHHQVLD
                     VRMVRVGTLGPGEIRAFKAKADTYDNLYNIASHEILPRGWE"
     gene            1353..2315
                     /locus_tag="PLS90_15980"
     CDS             1353..2315
                     /locus_tag="PLS90_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03947.1"
                     /translation="MAARAWRFRLAVLATLIAVAAGPLPAQEADVTGPVLPGPAGMTA
                     PLPNQVRLEARVLEWQVNDELEFDFAVRYTPNPGSAGILQAADFTLPADPSLTSAARL
                     FFDDLDVGSSGGFDAMIETLQRVGEVSILSKPSIVLTKSDIPADTPEAPGFVTNSPQP
                     APVQHRKLSNSVKIPYETTKSVGVQLASVTEYRDTGVTMDVAVLDIVDDEVTLDITTT
                     VTDLTGFINIGLNTQEQPMRVPTIDSRTMSSRLIVPDRTVFIAGLMKTRRESQRRRGI
                     PWVSELPVLGWFLSSKINANEESELVFLVKPEVLTPYSLIEPGN"
     gene            2337..3521
                     /locus_tag="PLS90_15985"
     CDS             2337..3521
                     /locus_tag="PLS90_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HPK03948.1"
                     /translation="MHRWVRKDGWPWLVALSLLLTPVHAQEAAVEVPAGQEPAVEIAP
                     ASPPEVQVEAPAEAIADAPEETIAESPVETTIAEAPAAAPAVLSAEEAALVRRNDNNR
                     HMVAIEALVIEINEERTRDLGIRYGFTQVTRNDAGVPVPGGPGVLEGGDVRLGRPLNP
                     VRVPVLLRQIDGVTDIGFEQQRMPGLGITLAGMNVGTGAVSAQLRTLLQSGDASIRTR
                     PIVVALNNTRATIEVADEVPYLDINEHSQVAVAFEPVGVRMAVIPLIKELRPGIVQLE
                     IENLEVSSVSSFITQQNVQRPVFNKSQTSTNVTLSEGETFVIGGLKTQHLTHIEERVP
                     ILGAIPGLGWLFKSQEDRERIMDVLFFITPYILEPGENFLLPYDFKNHQALGLNFNVA
                     RR"
BASE COUNT          727 a         1230 c         1341 g          661 t
ORIGIN      
        1 tcggcggcgg catctaccac gcctacgatt ccaatgtgat gtatcgcgcc gggggtctgc
       61 tggtgctggg cggggcgggg ctgctggggg tgagccggat gctctacggt cggggaaaag
      121 ggcgtttcgg ggccgctttt ttcagcggcg acgactcgag ataaatcatt taaaatcagt
      181 catatacaat cttgttagag cgaggctcgc gcctcgtcgc ggctattcgg gggagaaaaa
      241 accaaaccta tcccccaggg agggggtcgg gtcccatgag tcccctcgcc gagggccgga
      301 ttgtggtcaa cccggggggc gaaaccggag tgtccggcat cggatccatg aggagggccc
      361 cccgtcatgc gtttccccgc ggtccttctg tcgatgtccc tgctgctggc ctcgctggcg
      421 ccgctgaccg gctgcgtctg ggccacccag atgcgcctgc acgggcaact gaaccaggcc
      481 gagcgcgcgt tcgagcaggg caactacccg cttgccgcga cgcgttatga gagcctgagc
      541 aatcgctatc ccgacgggcc gatgcgtgaa aaaattctca tgcgccaagg catcgcctat
      601 tacaccgtgc aggcctatca cgaggcgcgc gatacgttcc tgcgctacct gcacgagtat
      661 ccgcaggggc actacgcggc cgacgccaac gagtacatcg ccaaggtgga tgttctgctc
      721 tcgccgggca atccggccga ggaggccgcg ctggccgcgg cactgcagga cctgaacgcg
      781 ctgcagaagc tgcgcatgga gcaccccacc gatccggcgg tcgcgacggc gatcggcgat
      841 ctctaccgcg atctgggcga ctatgacgag gcgatgcgct actactacga agcccaggaa
      901 ctgggcgcga cgttccagga gcgcgacctg ctgcaggcgc gcctgatgct cgacgccaac
      961 ggcgcccccg tgccgatgac gcccgaacag gtccgcctgc gcaacctgga ggacaacccg
     1021 ctggtgatct atgacgtgta tgattacaag gcgcgcgatc cgggggagaa cgggttcaac
     1081 aatcgcctgc aggattacgt cgtcaccggg cggatccgca accagagcag ccggatcatc
     1141 cacgatgtcg agctggaggt gcgctttctg aatgcgcatc atcaggtgct cgacgtccgc
     1201 atggtgcggg tcgggacgct ggggccgggc gagatccgcg ctttcaaggc gaaggccgat
     1261 acctacgaca acctgtacaa catcgccagc catgagatcc tgccgcgggg gtgggaatga
     1321 tgtctcggcg taacacaccg gggtcgccgc atgtggcggc tcgcgcatgg cgcttccggc
     1381 tggcggttct ggcgacgctc atcgcggtcg cggccggccc gctgccggcc caggaggccg
     1441 acgtgacggg gccggtgctg ccgggtccgg cggggatgac ggccccgcta ccgaaccagg
     1501 tccggctgga ggcccgcgtg ctggagtggc aggtgaacga cgaactggag ttcgatttcg
     1561 ccgtgcgcta cacgccgaac cccggttccg ccggcatcct gcaggcggcc gacttcacgc
     1621 tgccggccga cccgtcgctg accagcgccg cccggctgtt cttcgacgat ctggacgtcg
     1681 gcagttccgg cggcttcgac gcgatgattg agacgctgca gcgcgtgggc gaggtcagca
     1741 ttctttccaa gccgtcgatc gtgctgacca agtcggacat cccggcggac acgcccgagg
     1801 cgccgggatt tgtcaccaac agcccgcaac cggcgcccgt ccagcaccgc aagctctcca
     1861 attcggtcaa gatcccttat gaaacgacca agtcggtcgg cgtgcaactg gcgtcggtga
     1921 ccgaataccg cgataccggc gtgacgatgg acgtggcggt gctcgacatc gtggacgacg
     1981 aggtgacgct cgatatcacc acgaccgtga ccgacctgac cgggttcatc aacatcgggc
     2041 tgaacacaca ggaacagccg atgcgcgtgc cgacgatcga ctcgcgcacg atgagcagcc
     2101 ggctgatcgt gccggaccgg acggtgttca tcgccgggct gatgaagacc cggcgcgaat
     2161 cgcagcgccg ccgcggcatc ccgtgggtca gcgagctgcc cgtgctgggc tggttccttt
     2221 ccagcaagat caacgcgaac gaggaatcgg agctggtctt cctggtgaag cccgaagtgc
     2281 tgacgcccta tagcctgatc gagccgggga attaggccat gaatcgaggc gacgccatgc
     2341 atcgatgggt tcggaaagac ggctggccgt ggctggtggc gctgagcctg ctgctgacgc
     2401 cggtccatgc gcaggaagcg gcggtcgagg taccggcggg ccaggagccg gccgtcgaga
     2461 tcgcccccgc gagtccaccg gaagtccagg tggaagcgcc ggccgaagcc attgccgatg
     2521 cccctgagga aaccatcgcc gagagcccgg tggaaacaac catcgccgaa gcgccggccg
     2581 cggcgccggc ggtgctttcc gccgaggaag cggcgctcgt gcggcgtaac gacaacaacc
     2641 ggcacatggt ggcgatcgag gcgttggtga ttgaaatcaa tgaggagcgg acgcgcgacc
     2701 tgggcatccg ctatggattt acgcaggtga cccgcaacga cgcgggggtg ccggtgccgg
     2761 gcgggcccgg cgtgctggaa gggggggatg tccgcctcgg ccggccgctc aatccggtgc
     2821 gcgtcccggt gttgctgcgc cagatcgacg gcgtgaccga catcggcttc gagcagcagc
     2881 ggatgccggg cctgggcatc acgctggccg ggatgaacgt cgggacgggg gccgtctcgg
     2941 cgcagcttcg cacgctgctg caatcgggcg acgcctcgat ccgaacgcgg ccgatcgtcg
     3001 tggcgctgaa caacacgcgc gcgacgatcg aggtcgccga cgaagtcccc tacctcgaca
     3061 tcaacgagca cagccaggtg gcggtggcgt tcgagccggt cggcgtgcgg atggccgtga
     3121 ttccgctgat caaggaactg cggccgggga tcgtccagct tgagatcgaa aatctggaag
     3181 tctcatcggt cagttcgttc atcacgcagc agaacgtgca gcggccggtg ttcaacaaat
     3241 cgcagacgtc gaccaacgtg acgctgagcg agggcgagac gttcgtcatc ggcgggctga
     3301 agacccagca cctgacccac atcgaagaac gcgtgccgat cctgggcgcg atcccgggcc
     3361 tgggctggct gttcaagagc caggaagatc gcgaacggat catggacgtg ctattcttca
     3421 tcacgcccta cattctggaa ccgggtgaaa acttcctgct gccctacgat ttcaagaatc
     3481 accaggcgct cggtctgaac ttcaacgtcg cgcgccggta ggatcgaacc cgcgcgcgcc
     3541 tcgcccctcc cccccacggg gaacgccgtc acgcaagagc cattcgccag gagaggttcc
     3601 gtcaaatatt aatgaagctg atccggcgcg tatcgggaac cgccggtagt ggatgtcgat
     3661 tttttgccgt gaacttttca tgacatgtcg gtgttcaccc aggaaagcat gagaatgggg
     3721 cgcctgccca tgtttcgctg agattcgccc cgttcgggga cggggaaatt ctgcgttggc
     3781 gtccccaggt tccgctgcgc ttcaccaggg gctattcaaa tcgcgccccg ctggggcggc
     3841 tgacgcggac ggctgtttat gaaattgagt cggccgggga tcggacggcg catgcggacg
     3901 gcgctatgga ggaattgagt cgatgggtgg aagagagtga aattctcata cgagttgct
//
LOCUS       DAOPCO010000344         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18617_length_3957_cov_5.321630, whole genome shotgun sequence.
ACCESSION   DAOPCO010000344 DAOPCO010000000
VERSION     DAOPCO010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18617_length_3957_cov_5.321630"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..795)
                     /locus_tag="PLS90_15990"
     CDS             complement(100..795)
                     /locus_tag="PLS90_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HPK03949.1"
                     /translation="MAIGLSRREQIALLGTVAVILCFGLGARAWQRRGTAGLVFVDGQ
                     GAWEKLAEFEAGDRLPAWPTGEAQTTTGAGAGLPEGGIDLNTASAEELDRLPGIGPAR
                     AADILATRERLGGFATVGALLEVPGIGPATLEKLRPYVRVEQGAGEPIRPAGQFIPAT
                     PAALALPRININTADAAELTRIKGVGPVLAGRIIEHRRRHGPFRRVEDLLAVNGIGPR
                     TLEAMRPQIALAP"
     gene            923..1726
                     /locus_tag="PLS90_15995"
     CDS             923..1726
                     /locus_tag="PLS90_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPK03950.1"
                     /translation="MALVRIWLYHPPVMKRAIIRGARAGLRHARRWIMRMEFVTLVLL
                     VLLAGSIHFFVELADDVLEGDTRELDEWIILGMRNPDDLADPVGPPWLEEMIRDITGL
                     GGIGVLSLVTLGVAGYLGLAGKRHAMVLVLAAVFGGMVASQLLKWGFERPRPDLVPHH
                     SEVYTTSFPSGHSMVSAVTYLTLGALLARINSRRRIKTYVLCLAVLVTVAVGVSRVYL
                     GVHWPTDVVAGWSAGAAWAMACWLAAIWLQKRGAIEAPESPASVDAAKT"
     gene            1978..3024
                     /locus_tag="PLS90_16000"
     CDS             1978..3024
                     /locus_tag="PLS90_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK03951.1"
                     /translation="MKESIPPAAWVFSQGNWRARYRSRHTIVLSGGEGTRMRPFIRQW
                     LGEDRPKQYCAFVGTRTMLQHSLDRAADLVDPGHTHLVLGPQHQRYLGHGLPGFAGRL
                     HPQPANRGTAVGIFLALAEILEADPEATVLVLPSDQFIHPEAAFRRYADRAFLLAEQF
                     RDHVVLMGVAATEPERDYGWIEPAEQVLGANWEADLRPPSRVRRFVEKPSANLAELLL
                     QRRALWNTMSLVARAETLWKMGRRFLPRLVRRFDEYRLMRRALGADARRLEAAALLTR
                     IYEQPGQIDFCQAILQSDPQSCLVLPMDDLHWSDWGRPERIEDTILRFGIRPNIPLAL
                     PETRGVDIHEYAIG"
     gene            3092..3499
                     /locus_tag="PLS90_16005"
     CDS             3092..3499
                     /locus_tag="PLS90_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03952.1"
                     /translation="MLTRNVLLFEANPQTRLYVKDILDYFSFNVQTAETWDEAEQHLQ
                     HGDYDVMIVDEAHVHLGHATPVRWIRERGSDLPIIIMSYMPLPELWSNCLEHDGVELI
                     SNPFSPSELKLMLDIVTSKRIRKGSPELSSEQT"
     gene            complement(3612..>3957)
                     /locus_tag="PLS90_16010"
     CDS             complement(3612..>3957)
                     /locus_tag="PLS90_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPK03953.1"
                     /translation="ESHETFNRLTMATRQRIRAELSLAIHHGVSPLPVAEWTALLTEA
                     GFVEPRIWLAPFRLLEPSQVLRDEGWLRAARIGVNLLREAEARRRVVEMRRAFRRHQA
                     HLRAVVIIARKA"
BASE COUNT          623 a         1295 c         1365 g          674 t
ORIGIN      
        1 tcaggtgaca tttctattgt gtccaggggg tgacattatc attgtggccc gacaggcggc
       61 cgatgcgtgc ccttagggcc cgccggccag agcgcggggc tacggggcaa gtgcgatctg
      121 cgggcgcatg gcctccagcg tgcggggtcc gatcccgtta accgcgagca ggtcctcgac
      181 ccgccgaaaa ggtccgtgac ggcggcgatg ttcgataatc cggccggcaa ggaccggtcc
      241 gacccccttg atccgggtca gctcggccgc atcggccgta ttgatgttga tgcgcggcag
      301 ggcaagcgcc gcgggcgtcg cgggaatgaa ttgccccgcg gggcggatgg gttcgcccgc
      361 gccctgctcg acgcgcacgt aggggcgcag tttttccagc gtggccggcc cgatgcccgg
      421 cacttcgagc agggcgccga ccgtggcgaa gccgccgagc cgctcgcgcg tcgccaggat
      481 gtcggccgcc cgagccggcc cgatgcccgg caggcggtcc agctcctcgg cggaggcggt
      541 attgaggtcg atgccgccct cgggaaggcc cgcgccggcg ccggtcgtgg tttgagcctc
      601 cccggtcggc caggcgggca gcctgtcgcc ggcctcaaat tcggccagtt tctcccaggc
      661 gccctgccca tccacgaaga ccagaccggc cgtgccacgg cgctgccagg cacgcgcgcc
      721 cagaccgaag cagaggatga ccgcgacggt tcccaacagc gcaatctgtt cgcggcgtga
      781 cagaccgatc gccatggagg aaagctagtc attcgccggg cggaagtaaa tcgcgattcg
      841 aagatccgca gatcctgagg cagcatccgt cgactccgac cgatccgtcc ggtccgacgg
      901 attgtgtggg acggccggtg gtgtggcgct ggtgcgcatc tggctgtatc atccccctgt
      961 catgaaacga gcgatcattc gaggagcgcg cgccggcctc cgccatgccc gccggtggat
     1021 catgcggatg gaattcgtca cgctggtcct gctcgtgttg ctggccgggt ccattcactt
     1081 cttcgtcgag ctggcggatg atgttctgga gggcgacacg cgcgaactgg acgaatggat
     1141 catcctcggc atgcggaacc ctgacgatct tgcggatccg gtcggcccac cgtggctgga
     1201 ggagatgatc cgcgacatca ccggcctggg gggcatcggg gtcctgagcc tggtgacgct
     1261 gggcgtggcc ggctatctgg ggctggccgg caagcgccat gcgatggtcc tggtgttggc
     1321 cgcggtgttt ggcgggatgg ttgcctcgca actgctcaaa tggggtttcg agcgcccgcg
     1381 gccagacctg gtcccgcatc actccgaggt ctacacgaca agctttccca gcggccactc
     1441 gatggtgtcg gcggtgactt atttgacgct cggggcgctg ctggcccgga tcaacagccg
     1501 gcgccggatc aagacctacg tcctgtgtct ggccgtgctg gtgacggtgg ccgtgggggt
     1561 cagccgggtc tatctgggcg ttcactggcc gaccgacgtg gtggccggat ggtcggcggg
     1621 ggccgcctgg gcgatggcct gctggctggc ggcgatctgg ttgcagaaac gcggggcgat
     1681 tgaggcgccc gaatcgccgg ccagtgttga tgcggcaaaa acctgagtca gggagggcgc
     1741 cggcggaggt ttttacctgc aaagggaagc cttttcacgg ccgtcggaac aggcgccctg
     1801 cttccgcgat ccgatttccc ttctgattct ttcaccgccc gcccgatggc cgtttagggg
     1861 attccctgat ttccggcgcc ggccgcagcg gctagactcg ctgggtcatc gacgacccac
     1921 agcgcgcgcc cagtcgagcc gactcggact cagcaagcaa tgaaggactt cgcccccatg
     1981 aaggaatcga tcccgccggc ggcctgggtg ttctcgcagg gcaactggcg cgcccgttac
     2041 cggtcgcgcc acacgattgt cctgtccggg ggggaaggca cgcggatgcg gccgttcatt
     2101 cgccagtggt tgggggagga tcggccgaag caatactgcg cgttcgtcgg gacccggacg
     2161 atgctgcaac actcgctcga ccgcgcggcc gatctggttg atcccggcca tacgcatctg
     2221 gtcctcgggc cgcaacatca acgctacctc ggccatgggt tgcccggctt cgcggggcgg
     2281 ctccacccgc aaccggcgaa ccgggggacg gccgtgggca ttttcctcgc cctcgccgaa
     2341 atccttgagg cggaccccga agcgaccgtg ctggtcttgc cgtcggatca attcatccat
     2401 cccgaggcgg ccttccggcg ctacgccgac cgcgccttcc tgctggcgga acagtttcgc
     2461 gaccatgtcg tgctgatggg cgtcgcggcg accgaaccgg agcgggatta cggctggatc
     2521 gagccggccg agcaggtgct cggcgccaat tgggaggccg acttgcggcc gccgagccgc
     2581 gtgcgccggt tcgtcgagaa accgtcggcc aatctggcgg aactcctgct ccagcggcgc
     2641 gcgctgtgga acacgatgag cctcgtggcg cgggccgaga cgctctggaa gatggggcgc
     2701 cggttcctgc cgcggctcgt gcgccggttc gatgaatacc ggctgatgcg gcgcgcgctg
     2761 ggcgccgacg cccgccgact ggaggccgcc gcgctcctga cgcggatata cgagcagccc
     2821 ggccagatcg acttttgcca ggcgatcctg caaagcgacc cccaatcgtg cctggtgctg
     2881 ccgatggacg atctgcactg gtcggactgg ggtcgacccg aacggataga ggatacgatc
     2941 ctgcggttcg gcattcgtcc gaacatccct ctggcgctgc ccgagacccg aggggtcgac
     3001 atccatgaat acgcgatcgg ctagtcgcgg agcgcccagg ggtggtatga tcatgtccag
     3061 tcctcgattc cggttcacga aggaatccga catgctgacc aggaacgtgc tgctgttcga
     3121 ggcgaaccct cagacccggc tctacgtgaa ggacatcctg gactacttct cgttcaacgt
     3181 gcagacggcc gagacgtggg atgaagccga gcagcacttg cagcacggcg attacgacgt
     3241 gatgatcgtc gacgaggcgc acgtccacct gggccacgcg accccggtgc gctggatccg
     3301 cgagcgcggc agcgacctgc cgatcatcat catgtcgtat atgccgctgc ccgagctgtg
     3361 gtccaactgc ctggagcacg acggcgtgga actgatcagc aacccgtttt cgcccagcga
     3421 gctgaagctg atgctggata tcgtgaccag caagcgcatt cgcaagggca gcccggaact
     3481 ctccagcgaa cagacctgat ccatcaggcc tatcttcgcg ctcctccccc ccaggccgcc
     3541 gcgtcacccc cccgacgcgg cggccatttt ttcaggcagg gacggcccga tagcgatccc
     3601 gataccgatg atcaggcttt gcgcgcgatg ataacgacgg cgcgcagatg ggcctggtgg
     3661 cgccggaagg cgcggcgcat ctcgaccacg cggcggcggg cctccgcttc gcgcagcagg
     3721 ttcacgccga tccgcgccgc gcgcagccag ccttcgtcgc gcagcacctg gctcggttcc
     3781 agcagccgga acggggcgag ccagatgcgc ggctccacga acccggcctc ggtcagcagg
     3841 gcggtccact cggcgacggg cagcggcgag acgccatggt ggatcgccag gctcagctcg
     3901 gcgcggatgc gctggcgcgt ggccatggtc agccggttga aggtttcgtg tgactcg
//
LOCUS       DAOPCO010000345         3931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18781_length_3931_cov_5.050826, whole genome shotgun sequence.
ACCESSION   DAOPCO010000345 DAOPCO010000000
VERSION     DAOPCO010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18781_length_3931_cov_5.050826"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3344
                     /locus_tag="PLS90_16015"
     CDS             <1..3344
                     /locus_tag="PLS90_16015"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK03954.1"
                     /translation="QQTIYLYVDGKLARATPTQGDDHEFAMYRAIGLSGDAMTGRPIH
                     GFSGRIDAVAESTFEGPFKTEYFQLTDVRDTDPAPAAFRAIELDVPLRVEELDRVPNR
                     RTIVHEPAGWDEPTYHHGPLIAFFKGRCYVAWHAADRTELDPATGMIASSSDLEQWSA
                     PSHLPGLPRNLFVQDDALYLWDAEGGIHVTRDGESWETPAERRHRFAGDIAAVGYRTD
                     LGGHEPLTDPDTDAPVDPSSYLDARAMERGAGHPFVQLPDGRWLAPVSVRSSGSAADD
                     QGTWLCAPVSASPIDPASWTGGLIDCSESPLPGDAAGWLGPDGVLHYVSRCGPRLWHA
                     SSADGGASWTPLLPQPDFPDAPSYKVFGALPDGATFYVGNPYPASRSQLVLSLSADGW
                     RFDDSYLVRWERVAPRFPSPYKNFQPGYEGVSAAVHDGKLYVVYSVCRERIEVAVIEL
                     DALTRTDEAGRPRLAGAPGPHPGLSAPPESEDEGAAVDAEPDGVRPEWLLNFEEGSLS
                     ADGRYLPSLAAGEIAPQGDGIAAVNQSGQLKLLRSEGRGVLQVASRDGSGDGLLFEHR
                     VEGRASRVLEAIVRPTAPQASEARLISLNDWDDRPPGQIRFFGIGAFGGAAGAVYQLP
                     YARIEPTIGPFRPRSGPGHYYLRGADLVEPGEEFHHLALVHEADREALYIYVDGKPSK
                     SLHFAAAANEFPIFRGIGLSVRALYGGQGIGFDGLIDAVAESSFTGEFRTSYFQLLEG
                     GDERPEAPAVDRKLTLNVPYQLATHPDDDGSHVSFFRAAKSRLPRLQTDTRMVFYPNS
                     HRDLCYNHNVIAHWFKDRLYVSWHTGQRGEHSFYYGGFVTSSADFEHWSPRVWLPSTP
                     RHMHATEDALTVWQFEHYSTTEDGIHWKTVPKDENNAELFNYLSNHHHARLSDGRLMT
                     VDVGRPAVSREDDIAGYQAVVNFTSDPSGMSGWSNGRIDVSPCADVGEPAGYEGPDGA
                     LHYISRSGFHIFHAASYDRGQTWTPLLPQPDFPDNGSNKEFGVFPDGSIWYVGNPIFG
                     DRRLVVLGISRDGWNFDEVYTIRDEEFTARFPAPFKAERPGYEYPSAYYHDGKLYIVY
                     SWYREGVELSIVDVEQLGVLGRAEE"
     gene            3536..>3931
                     /locus_tag="PLS90_16020"
     CDS             3536..>3931
                     /locus_tag="PLS90_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HPK03955.1"
                     /translation="MAKDARQSVGHIVSHTHWDREWRWPVWTTRLQLIEFFKQLLELM
                     ERREDFRYYTLDGQSVMVTDYLEMAPEDRTRIARLVTAGRLFIGPWFTLPDPWPIAGE
                     CMVRNLLVGRKVCRELGGALSVGYTTFGWG"
BASE COUNT          670 a         1189 c         1365 g          707 t
ORIGIN      
        1 ttcaacaaac catctatctg tatgtggatg gcaagctcgc gcgcgcgacg ccgacgcagg
       61 gggacgatca tgaattcgcc atgtatcgcg cgatcggcct gtcgggcgac gcgatgacgg
      121 gacggccgat ccacgggttt tccggccgga tagatgcggt ggccgaatcg acgttcgaag
      181 ggccgttcaa aaccgaatac tttcaattga cggatgtccg ggacaccgac cccgcccccg
      241 ccgcgttccg cgcgatcgag ctggacgttc cgctccgggt ggaagagctc gaccgcgtgc
      301 ccaaccggcg gacgatcgtt catgaaccag cgggctggga cgagccgaca taccaccatg
      361 ggccgttgat cgcgtttttc aaggggcgct gctacgtcgc ctggcatgcc gccgaccgca
      421 ccgaactgga tccggccacg gggatgatcg catcctcatc cgatctcgag cagtggtcgg
      481 cgcccagcca cctgcccggc ctgccgcgca acctgttcgt gcaggacgac gcgctctatc
      541 tctgggatgc cgagggcggg atccacgtga cacgcgacgg cgagagctgg gagacgcccg
      601 cggagcgccg ccatcggttt gccggggaca tcgcggcggt gggctaccgg acggacctcg
      661 ggggccatga gccgttgacc gatccggaca ccgatgcgcc ggtcgatccg tcgtcgtatc
      721 tggacgcgcg ggcgatggaa cgcggcgccg ggcatccctt cgtgcaattg ccggacgggc
      781 gctggctggc gccggtcagc gtgcggagtt cggggtccgc cgccgacgac caggggacat
      841 ggctctgtgc gccggtctcc gccagtccga tcgaccccgc gagttggacg ggcgggctga
      901 tcgattgttc ggaatccccg ttgccgggcg acgccgcggg ctggttgggg cccgatgggg
      961 ttctgcatta cgtctcgcgc tgcggaccgc gcctctggca tgcctcgagc gcggacggcg
     1021 gggcaagctg gaccccgctg ctgccgcaac cggatttccc cgacgccccg agctacaagg
     1081 tattcggcgc gctgccggat ggagcgacgt tctacgtggg gaacccatat ccggccagcc
     1141 ggtcgcaact ggtcctgtcg ctgagcgcgg atggctggcg gttcgacgac agctaccttg
     1201 tccgctggga gcgggtagcg ccccgcttcc cgtcgccgta caagaacttc cagcccgggt
     1261 atgaaggggt ctccgcggcg gtccatgacg ggaagctgta tgtggtctac agcgtctgcc
     1321 gggagcgaat cgaggttgcg gtcatcgaac tggacgcgct gacgcggacg gacgaggcgg
     1381 ggcggccgcg cctggcggga gcccctgggc cgcacccggg gctttccgct ccgcctgaga
     1441 gcgaagatga aggcgccgcg gtggatgccg agccggatgg cgtccggcca gagtggctgc
     1501 tcaattttga ggaaggaagt ctgagcgcgg atggccgcta tctgccgagt ctggccgcgg
     1561 gggagatcgc gccgcaaggc gacgggatcg cagccgtgaa ccagagtggg caactgaagc
     1621 tgctgcgcag cgaagggcgg ggcgtgcttc aagttgcatc gcgggatgga tcgggggatg
     1681 ggctgctctt cgagcaccgg gttgaggggc gcgcctcgcg ggtgctggag gcgatcgtcc
     1741 ggcccaccgc cccgcaggcg tcggaggccc ggttgatcag cctgaatgac tgggacgatc
     1801 ggccgccggg acagatccgc tttttcggca tcggtgcgtt cggcggcgcg gcgggagcgg
     1861 tgtatcaact gccctacgcg cggattgaac cgaccatcgg gccgttccgt ccgcgctcgg
     1921 gcccggggca ctactacctg cgcggggcgg acctggtcga acccggcgag gaattccatc
     1981 atctggcgct ggtccatgaa gccgaccgcg aggcgcttta tatctacgtg gacgggaagc
     2041 cctccaagag ccttcatttt gcggccgcgg cgaacgaatt cccgatcttt cgcgggatcg
     2101 gtttaagcgt gcgtgccctg tacggcgggc aagggatagg gtttgatgga ttaatcgatg
     2161 ccgtcgccga gtcgtcgttc accggggaat tcagaacctc ctattttcag ctcctggaag
     2221 ggggcgatga gcgtcccgag gcgccggccg ttgaccgcaa gctgacgctc aacgttcctt
     2281 accagttggc cacccatccc gatgacgatg gaagccatgt ttcattcttc agggcggcga
     2341 aatcacgcct gcccaggctt caaaccgata cgcggatggt cttctacccg aattcgcatc
     2401 gggatctgtg ctacaaccac aatgtcattg cccattggtt caaggaccgg ctctacgtct
     2461 catggcacac ggggcagcgg ggcgaacaca gtttttatta tgggggcttt gtcacctcct
     2521 ccgcggattt cgagcattgg tccccgcgag tctggctgcc ctctacgccg cggcacatgc
     2581 acgccaccga ggacgccctc accgtctggc agtttgagca ttattcgacg acggaagacg
     2641 gcatccactg gaagaccgtg cccaaggatg agaacaacgc cgagctgttc aattatctct
     2701 ccaatcatca tcatgcccga ctctccgacg ggcggctgat gacggtggat gtcggccggc
     2761 cggccgtatc ccgggaggac gacattgcgg gctatcaagc ggtcgtgaat ttcacatcgg
     2821 atccaagcgg catgtcgggc tggagcaacg gacgaattga tgtgagcccg tgcgccgatg
     2881 tgggtgaacc agccggatac gagggtcccg acggcgcgct gcactacatc tcgcggagcg
     2941 gcttccatat cttccatgcg gcaagctatg atcgggggca aacctggacg ccgctgttgc
     3001 cgcagccgga ttttccggac aatggttcga acaaggagtt cggggtgttc cccgatggat
     3061 cgatctggta cgtgggcaat ccgatcttcg gcgaccggcg gctcgtcgtc ctcggcatca
     3121 gccgggacgg gtggaatttc gacgaggtct atacgatccg ggatgaggaa ttcaccgcgc
     3181 gattccccgc gcccttcaag gcggagcggc ccggctacga gtatccctcg gcctattatc
     3241 atgatggcaa gctgtacatc gtgtatagct ggtatcgcga gggggtggag ctgagcatcg
     3301 tcgatgtgga gcagttgggc gtgctcgggc gcgcggagga gtgaccaggg gtcgaaaagc
     3361 cgtggcggta attaagacgg gcataatcag ttgacgcgcc aaattggatg ctataaggtc
     3421 cgcgggaagc gtttggcgcc gtgcggaggc tgtgtgccgc tgcgccgggc gaccccgccc
     3481 gcgacgcgcc gccgcatggc caatccgcgc gcacacgaag gggaagacac gggtaatggc
     3541 caaagacgca cgccaatccg tcggacatat cgtttcgcat acccactggg accgcgagtg
     3601 gagatggccg gtctggacca cgcggctgca gctgattgag tttttcaagc agttgctgga
     3661 gctgatggag cggcgcgagg acttccgcta ttacacgctc gacggccagt cggtgatggt
     3721 caccgattac ctcgagatgg cgccggagga ccgcacgcga attgcgcggc tggtgaccgc
     3781 cggccggctg ttcatcgggc cgtggttcac gctgcccgac ccatggccga tcgccgggga
     3841 atgcatggtg cgcaatctgc tcgtcgggcg gaaggtctgc cgcgaactgg gcggagcgct
     3901 gtcggtgggt tatacgacgt tcggctgggg g
//
LOCUS       DAOPCO010000346         3912 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_18889_length_3912_cov_3.710137, whole genome shotgun sequence.
ACCESSION   DAOPCO010000346 DAOPCO010000000
VERSION     DAOPCO010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18889_length_3912_cov_3.710137"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /locus_tag="PLS90_16025"
     CDS             complement(<1..1132)
                     /locus_tag="PLS90_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016872961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPK03956.1"
                     /translation="MTHTPRFATIDGNEAAAYVAHRINEVCAIYPITPSSPMGELADA
                     WSAANRPNIWGHIPQVVEMQSEGGAAGALHGALQAGALCTTFTASQGLLLMIPNMYKI
                     AGELTPTVLHVTARSLACQALSIFGDHSDVMAARQTGFALLASASVQEAMDMALIAQA
                     ASLRSRIPFLHFFDGFRTSHEVSKIALIPEDVMRRMIPDELVAAHRDRALNPDRPVLR
                     GSAQNPDVYFQARETVNPFYAACPGIVQRVMDEFAELTGRAYKLFDYVGAPDAERVVV
                     LMASGCETADETVEALIARGEKVGVLKVRLFRPFDVHAFSAALPETVKALAVLDRTKE
                     PGAAGEPLYQDVINALFETIGHNGFALKTIPTVVGGRYGLSSK"
     gene            1627..2301
                     /gene="yqeK"
                     /locus_tag="PLS90_16030"
     CDS             1627..2301
                     /gene="yqeK"
                     /locus_tag="PLS90_16030"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPK03957.1"
                     /translation="MSLNFPALVQLLTDRLPTVMSPARVQHVLGVTHVAATLAGRHGL
                     DPERAALAGLLHDQFKEVEPDRLRRELEALGERIAPDDLDFPKVWHGARAAAYARHEL
                     GIAAADLLESVALHSTSDAAATPLTHLVFIADYAEPGRKRAEAAEILRVARKDLAAGY
                     RLALRTKIGYLLRRGRAALHPRALRALEEAFGPGELDQLAREAASEPENGQRKPSSEK
                     TFELSH"
     gene            2315..2701
                     /gene="rsfS"
                     /locus_tag="PLS90_16035"
     CDS             2315..2701
                     /gene="rsfS"
                     /locus_tag="PLS90_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPK03958.1"
                     /translation="MLSPQVEPGLTTLEQRIGRICALIDEHQGEAIHVLDLRGISDFT
                     DAFVIATVRNPTHMQALSKHLVERLRAGGLRPINRPDEGLGRWALLDYGDVIIHLFER
                     EAREYYDLDNLWGDAQTVAWEKLAMA"
     gene            2778..2936
                     /locus_tag="PLS90_16040"
     CDS             2778..2936
                     /locus_tag="PLS90_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03959.1"
                     /translation="MRPWVEMAPGAAVSIHGLGIEPLTRFWNMRGSDDPTPGVSLRTR
                     FHGGRRCC"
     gene            3204..>3912
                     /locus_tag="PLS90_16045"
     CDS             3204..>3912
                     /locus_tag="PLS90_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03960.1"
                     /translation="MATEKGKSSSVRNAEAILNHVQEYLAIRFDHAGRINPPIRTVKE
                     DLLYHELANVRHSLLGEKIYGPIRQVVADVLLGDDRKKREGILSRNDQNSIQVEIVSH
                     CQDERSEATRGQIKIHDLKTFYASINPALSDFMDLVETWIWWDLPDAVELARFELKLG
                     VLQMIRQGRFTPEMRKRYAQHIQTQAGGAEEQGPMSNEDIVRYEVDELKSIRERWAYR
                     RASERGYMYVLKRDEVPP"
BASE COUNT          705 a         1017 c         1525 g          665 t
ORIGIN      
        1 ccttggagga gaggccgtag cggccgccga cgacggtggg gatcgttttg agggcgaagc
       61 cgttgtggcc gatggtttcg aacagggcgt tgatcacatc ctggtagagc ggttcgccgg
      121 cggcgccggg ttccttcgtg cggtcgagga cggcgagggc cttgaccgtt tcggggaggg
      181 cggcggaaaa ggcgtggacg tcgaaggggc ggaacagccg gaccttgaga acgccgacct
      241 tctcgccgcg ggcgatgagg gcttcgaccg tttcgtcggc ggtctcgcag ccggaggcca
      301 tcaggacgac gacgcgttcg gcgtcgggcg cgccgacgta gtcgaagagt ttgtaggcgc
      361 ggccggtcag ttcggcgaac tcatccatca cgcgctggac gatgccgggg caggcggcgt
      421 agaaggggtt gacggtctcg cgggcctgga aatagacgtc ggggttctgg gcggagccgc
      481 gcaggacggg gcggtcgggg ttgagggcgc gatcgcggtg ggcggcgacc agctcgtcgg
      541 ggatcatgcg gcgcatgacg tcctcgggga tgagcgcgat cttcgagact tcgtgcgagg
      601 tgcggaagcc gtcgaagaag tggaggaagg ggatgcgcga gcggagggag gcggcctggg
      661 cgatcagcgc catgtccatt gcctcctgga cggaagcgga ggcgagcagg gcgaagccgg
      721 tctggcgcgc agccatcacg tcgctgtggt cgccgaagat cgagagggcc tggcaggcca
      781 gcgagcgggc ggtgacgtgg aggacggtag gggtcagctc gccggcgatc ttatacatat
      841 tcgggatcat cagcaggagg ccctggctgg cggtgaaggt ggtgcagagg gcgccggcct
      901 ggagggcgcc gtgaagggcg ccggcggcgc cgccctcgct ctgcatctcg acgacctgcg
      961 ggatgtggcc ccagatgttg ggccggttgg cggcggacca ggcgtcggcc agttccccca
     1021 tgggagaaga gggggtgatg ggatagatgg cgcagacctc attgatgcgg tgggcgacgt
     1081 aggcggcggc ttcgttgccg tcgatcgtgg caaagcgggg cgtgtgcgtc atggctcggc
     1141 catccttccg ggatctgaat ctggggcgtt gggcgctgat gcgtcagggt aaaaagggat
     1201 cagggagggt cccttacggg ccatttgccc gctgcgggga tcgtataata aaaagactac
     1261 gatatccaga tatattttgc gagaaaaaaa tgcagccggc tgggaacggg gagggtgttc
     1321 gtgcgtgccg gcgaggatgc cggcggtccc aagcggtgat tgggataacg gacggcatgg
     1381 tttcgagagc cgaggacgag ggacgagagc cgagagccga atcttgggga ggaacctggg
     1441 gggcgattgc gaggatgagg acgacgttgg ggcgatgggg cttgaggcgg gggagggggg
     1501 gccgtataat tggagccggg ccgccatgac gacaggcaat tggcccgatt cctgttttcg
     1561 agtggatgca tcgccgcacg gaggtcaacg cgccgcaaca cacggcagga tacccgcaga
     1621 caaccgatga gcctcaattt tccggccctg gtccagctcc tgaccgatcg cctgccgacg
     1681 gtgatgtcgc ccgcgcgggt gcagcatgtt ctgggcgtta cgcatgtggc ggcgacgctg
     1741 gcggggcgtc acggtctgga ccccgagcgc gcggcgctgg ccggcctgct tcacgatcaa
     1801 ttcaaggaag tcgaaccgga ccggctgcgg cgcgagctgg aggcgctggg cgagaggatt
     1861 gcgcccgacg acctggattt tccgaaggtc tggcatggag cgcgggcggc ggcctacgcg
     1921 cgtcacgagc tggggatcgc ggcggccgat ctgctggaga gcgtcgcgct gcattcgacg
     1981 agcgacgcgg cggcgacgcc gctgacgcat ctggtgttca tcgccgatta cgccgaaccg
     2041 ggacgcaagc gcgcggaagc ggcggagatc ctgcgcgtgg cgcggaagga tctggccgcg
     2101 gggtatcggc ttgcgctgcg gacgaagatc ggttatctgc tgcggcgggg ccgcgcggcg
     2161 ctgcatccgc gcgcgctgcg cgccctggag gaagcgtttg gtccgggaga attggaccag
     2221 ttggcccgcg aagcggcgag cgagccggag aacggtcagc ggaaaccgag ttcagagaaa
     2281 accttcgaac tgagtcatta gaagcaggag gtgcatgttg tcccctcaag tggagccggg
     2341 gctgacgacc ctggaacaac ggattggccg gatctgcgcg ctgatcgacg agcatcaagg
     2401 cgaagcgatc catgtgctgg atctgcgcgg gatcagtgat ttcaccgacg cgttcgtgat
     2461 cgcgacggtg cgcaacccga cgcacatgca ggcgctttcg aaacatctgg tggagcggtt
     2521 gcgggcgggg ggcctgcggc cgatcaaccg gcccgacgag ggcctggggc gctgggcgct
     2581 gctggactat ggggacgtga tcatccatct gttcgagcgc gaggcgcggg aatattacga
     2641 tctggataat ctgtggggcg acgcgcagac ggttgcctgg gagaagctgg cgatggcgta
     2701 aatgcgggtt gggtggtgtc gctgcgcgca acaagggaca ttatcgaagc gatcaatcgg
     2761 ttctcttgaa ttggcggatg cgaccgtggg ttgaaatggc gccgggggcg gccgtttcaa
     2821 tccacggctt gggtatcgaa ccgctgacgc ggttctggaa catgcggggg agcgacgatc
     2881 ccacgccggg agtgtcgctg cgcacgcggt tccacggcgg tcgacgctgc tgctgacgcg
     2941 cgacccgctc gattcaccgt ggcaatccga cggatccgac gtatccggcg gattgggccg
     3001 gtcggacgga tgttgaagag tttaagctga atttcttagg gggcaagtcg gcgcggcggg
     3061 gaaccgaaag cggttgctcc gggggggcgg ggcgtgtcat agtatatgca tatctgaatt
     3121 cgcgcgacgg ccttttcttg ggaaaggggg ctgccgctgt ccgatccgtt gatcaagttc
     3181 gaatcacaaa ggagcgtggc ggcatggcga ccgagaaggg caagagcagc agcgtcagga
     3241 atgccgaggc gatcctgaat catgttcagg agtatctggc aatccgcttc gaccatgccg
     3301 ggcgaatcaa tccgccgatc cgcacggtaa aggaggacct gctgtatcat gagctggcga
     3361 acgtgcgtca ctcgctgctg ggcgagaaga tctatggtcc gattcgccag gtggtggcgg
     3421 atgtgctgct gggggacgat cgcaagaagc gggaagggat tctctcgcgc aacgatcaga
     3481 attcgatcca ggtggagatt gtcagccatt gccaggatga gcgatcggag gcgacgcgcg
     3541 ggcagatcaa gatccacgat ctgaagacgt tctacgcgtc aatcaatccg gcgctgtcgg
     3601 atttcatgga cctggtggag acgtggatct ggtgggatct acccgacgcg gtggagctgg
     3661 cgcggttcga gttgaagctg ggcgtgctgc agatgatccg gcaggggcgt ttcacgccgg
     3721 agatgcgcaa gcgttacgcg cagcacatcc agacgcaggc gggcggggcg gaggagcagg
     3781 gtccgatgtc gaacgaggac atcgtgcgct acgaggtgga tgagctgaag agcatccggg
     3841 agcgctgggc gtaccggcgc gcttcggagc ggggttacat gtatgtgctc aagcgcgacg
     3901 aagtgccgcc gc
//
LOCUS       DAOPCO010000347         3888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19006_length_3888_cov_4.594052, whole genome shotgun sequence.
ACCESSION   DAOPCO010000347 DAOPCO010000000
VERSION     DAOPCO010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19006_length_3888_cov_4.594052"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /gene="treY"
                     /locus_tag="PLS90_16050"
     CDS             complement(<1..2259)
                     /gene="treY"
                     /locus_tag="PLS90_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HPK03961.1"
                     /translation="MTVAPSPPGVAPRIPLSTYRIQFHAGFRFADALALADYLADLGI
                     GDLYASPLLMARRGSLHGYDISDHSRLNPELGDEEDFRRLSDALRRRDLGLLLDMVPN
                     HMGIGEETNRWWMDVLEHGPASIHADYFDIDWHPAKAELADKILLPVLEDQYGVVLED
                     GKLQLAYDTDRAAFVLRYHDYLLPVAPRTWDAILKAVDEACAPPPAGVADDEDETLRE
                     LRSIRSAARHLPPRSAAAPAQIQERYRESDVIRRRLLRLHRDSPRLRDALDRALGWLN
                     GRPGEPASFDPLDRLIAEQAYRLACWRVATDQINYRRFFDINDLAALRTERAEVFKAT
                     HRLVLRLIAEGHVSGLRIDHPDGLRNPRKYFRDLQAHALAARRGVCDDRARADIDRIL
                     APYDPEYDAERPADGPPFYVVAEKILTEGEPLPREWAIHGTVGYDFLNQTTALFVDSR
                     REAAFDRLYARFTGQALDFAELAYRNRLAIMEGSLASEINALARRIERIAERHRRFRD
                     FTLNNFIFALREIIACFAIYRTYIESARQAVLTHDRAYIERAVAEARRRNFITGSTIF
                     DFLRETLLLQNADAFAEADRPALTDFLLRFQQLTGPVMAKGLEDTAFYVFNRLVSLNE
                     VGGDPRRFGHSVDAFHQASLARRRDWPHAMLAGTTHDTKRSEDARARINLLSEIPGLW
                     RKALGRWSRMNARAKRTIATYHDREAPAPSRNDEYLLYQALIATWPLQPMDDAALADY
                     RRRIGEYMRKATR"
     gene            complement(2261..>3888)
                     /gene="treZ"
                     /locus_tag="PLS90_16055"
     CDS             complement(2261..>3888)
                     /gene="treZ"
                     /locus_tag="PLS90_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HPK03962.1"
                     /translation="PASRHQPRGVHGPSALPDPDFAWTDAPWRGIPLARLILYELHVG
                     TFTPAGTFDAAIDRLDELAELGVTAIELMPIAQFPGERNWGYDGVFPFAAQASYGGEP
                     ALKRLVDACHARGLAVVLDVVYNHLGPEGNALAAFGPYFTDRYRTPWGLALNFDGPDS
                     DPVRRFFIENALHWTVECHVDALRLDAVHAIFDCSACPFLRELADVVHAAAEHLGRRI
                     HLIAESDLNDTRLVRSPRSFGFGLDAQWNDEYHHIIHALLTGERDGYYTDYDSLLQLA
                     RAWREGFIFTGQYHRHRRRRHGHPSADIDPPRFVVFIQNHDQVGNRMRGERLSALVDL
                     ESCKLAAALVLLSPFTPLLFMGEEYADPAPFLYFVSHGDPELIDAVRKGRAGEFSSFG
                     WRAEPPDPQSEQTFARSRVTPALRRQGPHALLWAYYRELIRLRKALPAITDPERTGTE
                     ILPDLERDTLVVIRRTARHAVLLLFNLADAPRDLTLPPATGAWRPLLDSAAADWGGPG
                     VARLHFPAPGDRAAITAARRSACLFELQAKDAP"
BASE COUNT          608 a         1042 c         1665 g          573 t
ORIGIN      
        1 gcgggtggcc ttgcgcatgt attcgccgat tcgccggcgg taatcggcca gagcggcgtc
       61 gtccatcggc tgcaacggcc aggttgcgat gagcgcctgg tagagcaggt attcatcgtt
      121 gcggctgggg gcgggggcct cgcggtcgtg gtaggtggcg atggtgcgct tggcgcgggc
      181 gttcatgcgc gaccaacggc cgagggcctt gcgccagagg ccggggattt cggagagcag
      241 gttgatgcgg gcacgggcgt cctcgctgcg cttggtgtcg tgggtcgtgc cggcgagcat
      301 ggcgtgcggc cagtcgcggc ggcgggcgag gctggcctgg tgaaaggcgt cgaccgagtg
      361 gccgaaccgc cggggatcgc cgccgacttc gttgagcgaa acgaggcggt tgaagacgta
      421 gaaggcggtg tcctccaggc ccttggccat gacggggccg gtaagctgct ggaagcggag
      481 gagaaaatcg gtgagggcgg ggcggtcggc ctcggcgaag gcgtcggcgt tctggagcag
      541 gagggtttcg cggaggaaat caaagatggt ggagccggtg atgaaattgc ggcggcgggc
      601 ctcggcgacg gcgcgttcga tgtaggcgcg gtcgtgggtc aggacggcct ggcgcgccga
      661 ctcgatgtag gtgcggtaga tcgcgaagca ggcgatgatc tcgcgcaggg cgaagatgaa
      721 gttattgagg gtgaaatcgc gaaagcggcg gtggcgctcg gcgatgcgtt cgatgcggcg
      781 ggccagggcg ttgatctcgc tggcgagcga gccctccatg atggcgaggc ggttgcggta
      841 ggccagttcg gcgaagtcga gcgcctggcc ggtgaagcgg gcgtagaggc gatcgaaggc
      901 ggcctcgcgg cgcgaatcga cgaacagcgc cgtcgtctga ttgaggaagt cgtagccgac
      961 ggtgccgtgg atcgcccact cgcgggggag gggttcgccc tcggtgagga ttttttcggc
     1021 gacgacatag aaggggggtc cgtccgccgg ccgctcggcg tcgtattcgg ggtcataggg
     1081 ggcgaggatc cggtcgatgt cggcgcgggc gcgatcatcg cagacgccgc ggcgcgccgc
     1141 cagcgcgtga gcctggagat cgcggaaata cttgcgcggg ttgcgcaggc cgtcggggtg
     1201 gtcgatgcgc aggccgctga cgtggccctc ggcgatgagc cgcaggacca ggcgatgggt
     1261 cgccttgaag acctcggcgc gctcggtgcg gagcgcggcg aggtcgttga tgtcgaagaa
     1321 gcggcggtag ttgatctggt cggtggcgac gcgccagcag gcgaggcggt aggcctgctc
     1381 ggcgatcagg cggtcgagcg gatcaaagct ggcgggttcg ccggggcggc cgttgagcca
     1441 gccgagcgcg cggtccaggg catcgcgcag gcgcggggag tcccggtgga ggcgcagcag
     1501 gcggcggcgg atgacgtccg actcgcggta gcgctcctga atctgagcgg gagcggccgc
     1561 ggagcgcggg gggagatgcc gcgcggcgga gcgaatgctg cgcagttcgc gcagggtctc
     1621 gtcctcatcg tcggccacgc cggcgggggg cggcgcgcag gcttcatcga cggccttcag
     1681 gatggcgtcc caggttcgcg gggcgaccgg cagcagataa tcatggtagc gcaggacgaa
     1741 cgcggcccgg tcggtgtcgt aggcgagttg cagtttgccg tcctcgagga cgacgccgta
     1801 ctggtcctcg aggacgggga ggaggatttt gtcggcgagc tcggccttgg ccggatgcca
     1861 gtcgatgtcg aagtagtcgg catggatcga ggcggggccg tgctcgagga catccatcca
     1921 ccagcggttg gtttcctcgc cgatgcccat gtggttgggg accatgtcga gcaggaggcc
     1981 gaggtcgcgg cggcgcaggg cgtcggacag gcggcggaag tcctcctcgt cgcccagttc
     2041 ggggttcagg cgggagtggt cggagatatc gtagccgtgg aggctgccgc gacgggccat
     2101 gagcaggggc gaggcgtaga ggtcgccgat gccgaggtcg gccagatagt cggcgagggc
     2161 gagggcgtcg gcgaagcgga agccggcatg gaactgaatc cgataggtgg agagggggat
     2221 gcgcggcgcg acgccggggg gtgatggggc gacggtcatg tcagggtgcg tctttcgcct
     2281 ggagttcgaa caggcaggcg gagcggcgcg cggcggtgat ggcggcgcgg tcgccggggg
     2341 cggggaagtg gaggcgcgcg acaccggggc cgccccaatc ggcggcggcc gaatcgagca
     2401 gcggccgcca ggcgccggtc gccgggggga gcgtcaggtc gcgcggcgcg tcggccaggt
     2461 tgaagagaag gaggacggcg tggcgcgcgg tccggcggat cacgacgagc gtgtcgcgct
     2521 cgaggtcggg cagaatttcg gtgccggtcc gctcggggtc ggtgatggcc ggcagcgcct
     2581 tgcgcaggcg aatgagctcg cgatagtagg cccagaggag agcatggggg ccctggcggc
     2641 ggagcgcggg ggtgacgcgc gaacgggcga aggtttgctc gctctggggg tcggggggct
     2701 cggcgcgcca gccgaaggag gagaattccc cggcgcgccc cttacgcacc gcgtcgatga
     2761 gttccgggtc gccgtggctg acgaaataga ggaagggggc ggggtcggcg tattcctcgc
     2821 ccatgaagag gaggggcgtg aagggcgaga ggaggacgag ggcggccgcc agcttgcagg
     2881 attcgaggtc gacgagcgcg gagagccgtt cgccgcgcat acgattgccg acctgatcgt
     2941 ggttctggat gaagacgacg aaccgggggg ggtcgatgtc ggccgaaggg tggccgtgac
     3001 ggcgccggcg gtggcggtga tactgcccgg tgaagatgaa gccctcgcgc caggccctgg
     3061 ccagttgcag gaggctgtcg tagtccgtgt agtagccgtc gcgctcgccc gtcaggaggg
     3121 catggatgat gtggtgatat tcgtcgttcc actgggcatc gaggccgaac ccgaagctcc
     3181 gcggcgaccg gacaaggcgg gtgtcgttga ggtcgctctc ggcgatgaga tggatgcggc
     3241 ggccgaggtg ctcggcggcg gcatgaacga catcggcgag ttcgcggagg aaagggcagg
     3301 cggagcaatc gaagatcgca tggacggcat cgaggcggag cgcatcgacg tggcattcga
     3361 ccgtccaatg gagggcgttt tcgatgaaaa agcggcggac ggggtcgctg tcggggccgt
     3421 cgaaattgag cgcgaggccc cagggggtgc ggtagcggtc ggtgaagtag gggccgaagg
     3481 cggcgagggc gttcccctcg gggccgagat gattgtagac gacgtcgagg acgacggcga
     3541 ggccgcgggc gtgacaggca tcgacgaggc gcttgagggc cggctcgccg ccataggagg
     3601 cctgggcggc gaagggaaag acgccgtcgt aaccccagtt gcgctcgccg gggaactgcg
     3661 cgatgggcat gagctcgatc gcggtgacgc ccagctcggc gagctcatcg agccgatcga
     3721 tcgccgcgtc gaacgtgccg gccggggtga acgtgccgac gtgcagttcg tagaggatga
     3781 ggcgggcgag cgggatgccg cgccacggcg cgtcggtcca ggcaaaatcg gggtcgggaa
     3841 gcgccgacgg gccgtggacc ccccgcggct gatggcggga ggcgggat
//
LOCUS       DAOPCO010000348         3880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19061_length_3880_cov_5.569673, whole genome shotgun sequence.
ACCESSION   DAOPCO010000348 DAOPCO010000000
VERSION     DAOPCO010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19061_length_3880_cov_5.569673"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..1200
                     /locus_tag="PLS90_16060"
     CDS             361..1200
                     /locus_tag="PLS90_16060"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HPK03963.1"
                     /translation="MTASQTQQIDTLLRIQTLLPQLTEKERDVALYILEHDEVIYQTI
                     TQVVKSCGASYGSVDRFCKKLGCSGFQDLKIALARDQSSRAASRAEDGEGDPLQRAAR
                     AARRDIDCTLKLLDRAAVEKAARVLVKAEFVMVAGVSSSAGTALGIDYRLTRYGIASA
                     CLIDNHMQRHRAATLTSRDAAFLLSYSGSTREILATAEIAASSGATVIGLTNYAESPL
                     AEAADICLVTGIRTDPLTAEIASKVATEFVVTALFESVARLKRNAMDVLTTTFEATAD
                     RQL"
     gene            1770..3011
                     /locus_tag="PLS90_16065"
     CDS             1770..3011
                     /locus_tag="PLS90_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK03964.1"
                     /translation="MPYQLLRFALNKKYPEPCMFPTPERLKPRYDVVIIGGGGHGLAC
                     AYYLARDHGIRNVAVLEKSYIGAGGTGRNTAIVRSNYLTPEGVALYDESVRLFQDLSD
                     DLDLNLFYSRRGHFTLAHSDASLRTMRWRAEVNKHLGVDSEVVDRQFIKAKCPEIDLD
                     CGHNAPVMGALYHPPGAIARHDAVAWGYGRAACQRGVEIHQGTTVTGIRTEGDRVTAV
                     ETNRGVIETRCVLSAVAGYTPRITSMVGLRTPITVHPLQACVTEPLKPWLNVIMVSAS
                     LHIYVSQSSRGELVMGASLDPYEVHSTRSTLDFVEGLAGHLLELFPFLWDVKLLRQWA
                     GLSDMTPDFSPIMGTTRIDGFLIDAGWGTWGFKATPVSGKTMAWTIAHGRDHDLVKPF
                     RLSRFEEFDLVGEKGAASVGH"
     gene            3013..3276
                     /locus_tag="PLS90_16070"
     CDS             3013..3276
                     /locus_tag="PLS90_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009727198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HPK03965.1"
                     /translation="MKLLRCPINGLRPIQEFNYGGEYRPMPDPDASTDAQWADYVFNR
                     DGAPGVRLEWWYHLASGVWFLAEHDTARDVFLRTFLFEELGQG"
     gene            3273..>3880
                     /locus_tag="PLS90_16075"
     CDS             3273..>3880
                     /locus_tag="PLS90_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPK03966.1"
                     /translation="MSRRLAPQPGEWIDRNRPVTFRFEGRPYSGFAGDTITSALWAAG
                     VRVLGRSFKYHRPRGLYGLDGSDSNAMFETRDVVNIRGDRAPIEEGMDLRAVNTFGGV
                     ERDRLRIMNRFGRFTPVGFYYKAFHRPRSLFPFFENQIRRVAGLGTVNSGKALRHTPK
                     DYAFCDVLVIGAGPAGLAAALAAADAGARVLLVDESPRLGGSL"
BASE COUNT          697 a         1269 c         1259 g          655 t
ORIGIN      
        1 gggcggtctc ttctggggat gcggtggttg ccatgggaag tgtccgtggt ggatgccgac
       61 aggatcgaag cgcgggcggg atatcgcccc ggagaaccgt gtgtaaccga tcagtgacgt
      121 atgatgcatc atgcgcccga atccgatcaa gcgcaattca agcgccgctc aaaacccttc
      181 tctgcccttg gattgtctat accccaccgg tcctgcatcg catcccgcca gggatgccag
      241 agcgtagccg tgtggttgac cgcagcgcgg tcacttttgc acgaaaaaaa aagttgcctg
      301 cgccgcccgc tggagatatt atccacatca acctcaggta tcgaaacatt ctccggcgag
      361 gtgacggcgt cgcagaccca gcagatcgac accctcctgc gcatccagac cctcctgccg
      421 cagttgactg aaaaggaacg tgacgtcgcg ctctacatcc tcgagcacga cgaggtgatt
      481 taccagacga tcacgcaggt ggtgaaaagc tgcggcgcga gctacggctc cgtcgaccgc
      541 ttctgcaaga aactgggctg ttcgggcttt caggatctga agatcgcgct ggcgcgggat
      601 caatcctccc gcgccgccag tcgcgccgag gatggcgagg gcgatccctt gcagcgggcc
      661 gcgcgcgccg cccgccgcga catcgactgc acgttgaaac tgctcgatcg tgccgccgtc
      721 gaaaaggccg cccgcgtcct ggtcaaggct gaattcgtga tggttgccgg cgtcagcagt
      781 tcggccggca cggccctggg gatcgattac cggttgaccc gctacgggat cgcctcggcc
      841 tgcctgatcg acaaccacat gcagcgccat cgcgcggcga ccctgaccag ccgcgacgcg
      901 gcgttcctgc tgtcctactc gggcagtacc cgcgagattc tggcgacggc cgagatcgcc
      961 gcctccagcg gcgcgacggt gatcggcctg accaattacg ccgaatcgcc cctggccgag
     1021 gcggccgaca tctgcctggt gacggggatc cgcaccgacc cgctcaccgc cgagatcgcc
     1081 tcgaaggtgg cgactgagtt cgtcgtcacg gcgctgttcg aaagcgttgc gcggctcaag
     1141 cgcaacgcga tggatgtgct gacgacgacg ttcgaggcga ccgccgatcg gcagttgtag
     1201 cgggcggtcg tgacgaacag gccggcgtcg atcggccgac attcggaggg ccgatcacga
     1261 cgagcgcctg aatccgcggc ccacaacgag gggttcaaga aggaagccct tgaacgaccg
     1321 actccagcgg gtcaggcctt gacggattcg acccgccgcc agtcccgtca gcgacggcag
     1381 ggaatgccac cactcggcgc cgccgaaaat ctccgccgcc agtcccgtca gggacggtag
     1441 acttcagccc gccacttcag tggcgggaac cggcgagcaa ggaaacgaaa gagtcccgca
     1501 gggacgacag aaccatctga ttcgatcgcg attacgagtt gctcatgcac cggcggttca
     1561 caacgaggga tgaaaatggg ggggggcgat tcgaacgatt caggccttgc cggacctatc
     1621 agcagggcag tcccatcagg gacggcagac ttcagcccgc cacttcagtg gcgggaaccc
     1681 gcagggacga cagaaccgtt tgattcgatc acgaagacga gaacgaaaga cgattttgga
     1741 gcgcaggacg ttttcttgag gggaagtcga tgccgtatca attgctccgt ttcgcgctga
     1801 acaagaagta cccggagccg tgcatgttcc cgacgccgga gcgcctcaaa ccgcgctacg
     1861 acgtcgtgat tatcggcggc ggcggccatg gcctggcctg cgcctactac ctcgcgcgcg
     1921 accacggcat tcgcaacgtg gcggtgctgg agaagagcta catcggcgcg gggggaaccg
     1981 gccgcaatac ggccatcgtc cgctccaatt accttacgcc cgaaggcgtg gcgctctacg
     2041 acgaaagcgt ccggctgttc caggatctgt cggacgacct cgacctgaat ctgttctatt
     2101 cgcggcgcgg gcatttcacg ctggcccact ccgacgcgtc gctgcggacg atgcgctggc
     2161 gcgcggaggt caacaagcac ctgggcgtcg acagcgaggt cgtcgaccgc cagttcatca
     2221 aggccaaatg ccccgagatc gatctggatt gcggccacaa cgcgccggtg atgggggcgc
     2281 tctatcatcc gccgggcgcc atcgcccgcc acgacgccgt ggcgtggggc tacggtcgcg
     2341 cggcctgcca gcgcggcgtc gaaatccacc agggcactac cgtgaccggg atccgcaccg
     2401 agggcgaccg ggtgaccgcc gtagagacca atcgcggcgt aatcgagacc cgctgcgtgc
     2461 tctcggcggt ggcgggttac acgccgcgca tcacctcgat ggtcggcttg cggacgccga
     2521 tcaccgtcca tccgctgcag gcctgcgtca ccgagccgct caagccgtgg ctcaacgtga
     2581 tcatggtttc cgcctcgctg cacatctatg tgagccagtc ctcgcgcggg gagctcgtga
     2641 tgggggcgtc gctcgatccg tacgaagtcc actcgacgcg gtcgacgctc gacttcgtcg
     2701 aaggtctggc gggccacctg ctggaactct tcccgttcct ctgggacgtc aagctgctgc
     2761 ggcagtgggc gggcctctcc gacatgacgc cggacttttc gccgatcatg gggaccacgc
     2821 gcatcgatgg cttcctgatc gacgccggct ggggcacgtg gggcttcaag gcgacgccgg
     2881 tcagcggcaa gacgatggcc tggacgatcg cccacggccg cgatcacgat ctggtcaaac
     2941 ccttccggtt gagtcgcttc gaggaattcg atctggtcgg cgaaaaaggc gccgcttcgg
     3001 tgggacacta agatgaaact gcttcgatgc ccgatcaacg gcctgcggcc gatccaggaa
     3061 ttcaactacg gcggggaata tcggccgatg cccgatcccg acgcctccac cgacgcgcaa
     3121 tgggccgatt acgtcttcaa ccgcgacggc gcccccggcg tgcgcctcga atggtggtat
     3181 cacctggcca gcggcgtctg gttcctggcc gagcacgaca ccgcccgcga cgtgttcctg
     3241 agaacctttc tgtttgagga gttggggcag ggatgagcag acgactggcg ccgcaaccgg
     3301 gtgagtggat cgaccgcaat cggccggtga cgttccgttt cgagggacgt ccgtattccg
     3361 gcttcgccgg cgatacgatc accagcgccc tgtgggcggc gggcgtgcgc gtcctcggac
     3421 gcagcttcaa gtaccaccgg ccgcgcgggc tctacggcct cgacggcagc gacagcaacg
     3481 cgatgttcga gacgcgcgac gtggtcaata tccgcggcga ccgcgcgccg atcgaggagg
     3541 gcatggatct gcgcgccgtc aatacgttcg gcggcgtcga gcgcgatcgc ttgcggatca
     3601 tgaaccgctt cggccggttc acccccgtgg gcttctacta caaggcgttt catcggccgc
     3661 ggagcctctt cccgttcttc gaaaatcaga ttcggcgcgt cgcgggcctc ggcacagtca
     3721 acagcggcaa ggcgctccgc cacaccccga aggactatgc cttctgcgac gtcctcgtca
     3781 tcggcgccgg tccggccggg ctggcggcgg cgctggccgc ggccgacgcc ggggcccgcg
     3841 tgctgctcgt ggatgaatcc ccgcgcctgg gcggttcgct
//
LOCUS       DAOPCO010000349         3871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19127_length_3871_cov_4.689727, whole genome shotgun sequence.
ACCESSION   DAOPCO010000349 DAOPCO010000000
VERSION     DAOPCO010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19127_length_3871_cov_4.689727"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..964)
                     /locus_tag="PLS90_16080"
     CDS             complement(209..964)
                     /locus_tag="PLS90_16080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPK03967.1"
                     /translation="MRLNRFLAAAGLGSRRAVEQMIRDGRVAVNGQTVTQLATQVDER
                     RDRVELDGRPVRPAAQTLYLMLNKPRGYDVTRGGRHHHRRVYDLLPREVHPSVQAVGR
                     LDRNSTGLLLFTNDGDLNFRLTHPRHGCHKHYEVLVEGVVDDETLEIFREGIRLEDGP
                     AQAVEVARLEPTGPETTRLRIVMAEGRKHIVRRMCEAAGHPVLELDRTAVGPVRLGDL
                     ARGKVRPLTRREVAALKRSVGLTADPPTRSGRP"
     gene            complement(961..2178)
                     /gene="scpB"
                     /locus_tag="PLS90_16085"
     CDS             complement(961..2178)
                     /gene="scpB"
                     /locus_tag="PLS90_16085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPK03968.1"
                     /translation="MARRKTSNSISPDPPSVAAEPGPEPDPAADPDEISEPSPPADLE
                     TAPAEPPADEPTGTAQVEELTPDASANPANAPDEPLLGAGDLDEESAEIVDSQLGALL
                     RTPLGLRGAIEAILFAAPEPVGLRRMCNILGINDSRLVSAALQQLQADYDADRRGVQI
                     VESAQGYQMATREMFGELILRLRGKKRRPPLSPAALETLSIIAYRQPIIRAEIEAIRG
                     VESSGTIRNLIDMGLVEMVGRKDVLGRPPMYGTTETFLAAFGLKALEELPSIPELRAR
                     IDEQVRAEEDTARPPAPAVPAAPEPSPADDEAREPAADGSATPVADEEHADSSTPGAP
                     ERAEDDPEEGWDEGAGATDGEDGSEEDEDGDGEDEVDDPDDLEDEDEDEDEVDDELDD
                     EDDEADADEDARR"
     gene            2886..3740
                     /locus_tag="PLS90_16090"
     CDS             2886..3740
                     /locus_tag="PLS90_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03969.1"
                     /translation="MMSRTQPARFRSWVALLVALAAGVCAVPARAQLVRLSLEELVDK
                     ADNVFVAECVEKQCEFRDGLIMTRYRLKVEENWKGQVKTDKDGTVELVEPGGTLNKPV
                     PVTQFMLGMANMDKGEKVLLFTEKSEELARKAAGGETTRYPPDNPFIVGRYQGRFTVF
                     KHPETGEKLVVNPGPAVVPGSIGRSFERQLSVLTEENVAKAVAEREAAKTAGTADASG
                     DAAWRNWLAQRLLTRGGELAKRIEKRREADRRRVAQRSKADPKHAADIVQYEPLADVR
                     ARVAAMSQ"
BASE COUNT          608 a         1194 c         1429 g          640 t
ORIGIN      
        1 ggccattgtg cgcgtggcaa ggtcttttgc cttgagatcc cttgttcccg tcggcggcgg
       61 cggaaggtgc ggccgtcgcc tggatggctg attcaggcgc ggttaacgca cttccaagcg
      121 tatcggccgc cgggcgcacc ggacttgagc ccggcaagcc gccgcgtacg gcgccgccgg
      181 ttcggattcg ggggacgagg ggagttggtc agggccgacc ggatcgggtc gggggatcgg
      241 cggtcagccc gactgatcgt ttcagcgcgg cgacctcgcg tcgggtcaaa gggcgcacct
      301 tgccccgcgc caggtcgccc agccgcaccg gcccgacggc cgtccgatcg agctccagca
      361 ccggatgacc agccgcttcg cacatccgcc ggacaatatg tttgcgtcct tcggccatca
      421 cgatccgcag ccgcgtcgtc tcggggcccg tcggctcgag gcgcgcgacc tccaccgcct
      481 gggccggtcc gtcctccagc cggatcccct cgcggaagat ctccagcgtt tcgtcatcga
      541 ccaccccctc cacgagcact tcatagtgct tgtggcagcc gtggcgcgga tgggtcagac
      601 ggaaattcag atcgccgtcg ttggtgaaca gcagcaggcc ggtcgaattg cggtccagcc
      661 ggccgacagc ctgaaccgac gggtggacct cgcgcggcag caggtcgtag acgcgccggt
      721 ggtgatgacg gccgccgcga gtgacgtcgt agccgcgcgg cttgttcagc atcaggtaga
      781 gggtctgtgc cgccggccgg accgggcggc cgtccagctc gacgcggtcg cgccgttcgt
      841 ccacctgggt cgcgagttgc gtcaccgtct ggccgttgac ggcgacgcgg ccgtcgcgaa
      901 tcatctgctc cacggcgcgc cgcgacccga ggccggcggc ggccaggaag cggttaagcc
      961 tcatcggcga gcatcctcgt ccgcatccgc ctcgtcgtcc tcgtcgtcga gctcatcgtc
     1021 gacttcatct tcgtcctcgt cttcatcctc gaggtcgtcc ggatcgtcga cttcgtcctc
     1081 gccatcccca tcctcgtctt cctcggagcc gtcttcgccg tcggtcgctc cggctccctc
     1141 gtcccagcct tcttccggat cgtcttccgc ccgctccggc gctccaggag tagatgaatc
     1201 cgcgtgctcc tcatcggcaa ccggtgtggc cgagccgtcg gccgcgggtt cccgcgcctc
     1261 gtcgtcggcg ggggagggct ccggcgccgc ggggacggcg ggagccggcg ggcgggcggt
     1321 atcctcttcc gcgcggacct gttcgtcaat ccgcgcgcgc agttcgggga tcgacggcag
     1381 ttcctcgagg gctttcaggc caaatgccgc caggaaggtt tcggtcgtgc cgtacatcgg
     1441 cgggcggccg aggacatcct tgcggccgac catctcgacc aggcccatat cgatcaggtt
     1501 gcggatggtg ccgctggatt cgacgccgcg gatcgcctcg atctcggcac ggatgatcgg
     1561 ctggcggtag gcgatgatcg acagggtctc cagggcggcg ggcgagaggg ggggacggcg
     1621 cttcttgccg cgcagccgca ggatcagttc gccgaacatc tcgcgcgtgg ccatctggta
     1681 gccttgcgcc gactcgacga tctggacgcc gcggcggtcg gcgtcgtagt cggcctggag
     1741 ctgctggagc gcggccgaca cgaggcgtga atcgttgatt cccaagatgt tgcacatgcg
     1801 gcggaggccg accggctcgg gcgcggcgaa caggatcgct tcaatcgcgc cgcgcagccc
     1861 caacggcgtg cgcagcagcg cgccgagctg gctgtcgacg atctcggccg attcctcgtc
     1921 caggtcgccg gcgcccagga ggggttcgtc cggggcattt gcggggttgg cgctcgcgtc
     1981 gggggtcagt tcctctacct gggcggtccc ggtcggctcg tcggcgggtg gttcggccgg
     2041 ggccgtttcc aggtcggccg gcggggaggg ttcgctgatt tcatccgggt ccgcggcggg
     2101 atccggctcg gggccgggct cggccgcgac ggacggcgga tcgggtgaaa tcgagttgct
     2161 ggtcttgcgt cgggccatcg tgcgtggggg tccgtggcgg gggtttgact cggcggaggg
     2221 ccgagccagg cgcacattca taaaactctc tatcggcgcg gcggagcggg tcatgcttcc
     2281 cttggccggg gcgtgcgtcc cattatgccg atgcgtggga caaaaccaag cgggaattgg
     2341 gaggggcagg aatcgagccg gaaaggatat aaaggactaa aaaaacataa ataatataaa
     2401 taggtataaa gaaaaaataa atacaattcg ctcacgagga cgagggacga gagacggttc
     2461 ttgaagtggg ggcgtgcctc tgctggtgct tgtgggttgg cgggtggggg gaagtgccat
     2521 acactgcgaa ggcgagggat gcgctggccg gcgcagccgg agggtcccgc ccaagtcgcg
     2581 ggacgctccc agggcccgca gcaggtgtcg agtcgttccc cccgcgacgc gataatctaa
     2641 ggcttgacag ccctataggt ccaattatga tggctggagc cagtgcgccg attggacccg
     2701 gtcgcgggcc gattgtgcat gctcggaacc cgcgttgatc agtccctgcc gagcgcgaga
     2761 ccgccaatcg ggaccctcgc cattttccca atgcatcagc tgcgaaagcc acatcgtcgg
     2821 tcggccgcca gggagtgcaa cggcagcgcc gcaaggctgg cgcgacgcag ttacggaggt
     2881 ttagcgtgat gtcccgaacc caacctgccc ggttccggag ttgggtggcc ctgctggtcg
     2941 ccctggcggc cggggtgtgc gccgtcccgg cacgggccca gttggtccgg ctgagcctgg
     3001 aggaactggt ggataaggcg gacaatgtgt tcgtcgcgga atgtgtcgag aagcaatgcg
     3061 agtttcgcga cggcctgatc atgacgcgct accggttgaa ggtggaggag aactggaagg
     3121 ggcaggtcaa gaccgacaag gatgggacgg tggaactggt ggagccgggc gggacgctca
     3181 acaagccggt gccggtgacg cagttcatgc tcgggatggc gaacatggac aaaggggaga
     3241 aggtcctgct gttcacggag aagagcgagg aactggcccg gaaggcggcg gggggcgaaa
     3301 cgacgcgcta tccgcccgat aatccgttca tcgtggggcg gtatcagggc cgattcacgg
     3361 tcttcaagca ccccgagacg ggggagaagc tggtggtcaa cccgggtccg gcggtcgtgc
     3421 cgggcagcat cgggcggtcg ttcgagcgtc agttgtcggt gctgaccgag gagaacgtgg
     3481 ccaaggcggt tgcggagcgc gaggcggcca agacggccgg aaccgccgac gcgtcgggtg
     3541 acgcggcgtg gcgcaactgg ctggcgcagc ggctgctgac gcgcggcggc gaactcgcca
     3601 agcggatcga aaagcggcgg gaggctgacc ggcggcgggt cgcgcagcgc agcaaggcgg
     3661 acccgaaaca cgcggccgat attgtgcagt acgagcccct ggccgacgtc agggcgcgcg
     3721 tggcggcgat gagccagtag cgcgagcggc tggcggcggg cgggcgatgg gccgcaccgg
     3781 acggtctcag cgcggcgcga tcggacattg gggaaacagc gcgagaatcg gggattcagg
     3841 atgaaacatg ccccatcggc aggccggggt g
//
LOCUS       DAOPCO010000350         3849 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19253_length_3849_cov_4.087507, whole genome shotgun sequence.
ACCESSION   DAOPCO010000350 DAOPCO010000000
VERSION     DAOPCO010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19253_length_3849_cov_4.087507"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..414)
                     /locus_tag="PLS90_16095"
                     /pseudo
     CDS             complement(1..414)
                     /locus_tag="PLS90_16095"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(402..1361)
                     /locus_tag="PLS90_16100"
     CDS             complement(402..1361)
                     /locus_tag="PLS90_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPK03970.1"
                     /translation="MAELAAAPSPRPPLPAARRSHWLLRLIRWIFRTIAEFIRFWLYI
                     ILFFAVIVLVSYQVVAHYIRGEEILAPDLTGLSVLEALRTMREDGLMLVLDGEQPHPT
                     QREGQVISQIPAPGTRIKAGTPVRVLVSSGQALVTVPDLRGETKIPAGIRLRNLGLDV
                     GTIAEIEEPGEPSGAILQTDPPGGTGVPEGTRVNLLVVKGRQSEFERVPNLLGMTLDQ
                     ARIALSAQGLVLGEIREAPSDEVNVGEIHEQQPPPGETVLAQTSVSVTYVPSPSVEAE
                     VEYYELPRVDLDFSEEEEPDRPAATPAVPLPEETPREPSMWRR"
     gene            1709..2323
                     /locus_tag="PLS90_16105"
     CDS             1709..2323
                     /locus_tag="PLS90_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPK03971.1"
                     /translation="MAFDQLSSYQIGKMLDVSRQAVNQWIDKGYIPSYRTPGGHRRVR
                     REDFINFLNSRQIPIPDILQRAIDQERRSAAPRIILVDDDAEFLAVLRQAIQEQLPEA
                     RIEAYESGYDALVAIGQGLPDLLVLDIKMPKIDGVEVCRRLKRNDATRRLPVMIVSAH
                     DTPEWRESLQALKVQAIFSKGEPLLEIARQIRQFIETKAATLKI"
     gene            2615..3322
                     /locus_tag="PLS90_16110"
     CDS             2615..3322
                     /locus_tag="PLS90_16110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPK03972.1"
                     /translation="MAQPDSVTQWQLQVVSEHTVHCRQRYFRTKTLFDWCAAASGLVL
                     LAPVMFVIALAVRLTSRGPILYRQIRVGINNQPFLIYKFRTMRVDAEKDGAQWARRND
                     PRVTPIGRFLRRTHLDELPQLINVLKGEMSLVGPRPERPIFVSELSRQIEGYPLRLAV
                     KPGITGLAQVHHRYDESIEDVKVKLHYDLAYVQSCGFGMDLKIIWNTFWSLLTGRTHD
                     VGAPSGATTGADSAKAA"
BASE COUNT          640 a         1097 c         1430 g          682 t
ORIGIN      
        1 ggggtagagg tccaggccgc ccagatggaa gtaaatcgcg ttgatgaagc gctgcttgtt
       61 gcggaagcct cgggcccgca ctttcaccag cttgatccgg ctgttgatcg actctgctcc
      121 gccgttgtgt gccttgagca ccacggcatt aataattcac cataaatgct cgcggatcat
      181 ccccaccacc ttgcgtattg gttcctgacg gttgcggccg gcccaactca accaggcgcg
      241 ccacgcccgc tcggcccatc cgcggctgac gtaatgccat aggctcatcg ccgtctcctt
      301 cagcgcccag gccctggcga tgccgatccc gatagcgatc ccatcccgat cccgatggcg
      361 ataccgatat tgaaggcgat accgatcgcg gttcaggggc gtcagcggcg ccacatggag
      421 ggttcgcgag gggtttcctc ggggagggga acggcggggg tggcggcggg gcggtcgggt
      481 tcttcctcct cgctgaaatc gagatcgacg cgcggcagtt cgtagtattc cacctcggcc
      541 tcgaccgacg gggacgggac ataggtaacg gagacggagg tctgggcgag gacggtttcg
      601 ccggggggcg gctgctgctc gtggatctcg ccgacgttga cctcgtcgga gggggcctcg
      661 cggatttcgc ccaggacgag gccctgggcg gagagggcga tgcgcgcctg gtcgagcgtc
      721 atgccgagca gattggggac gcgctcgaac tcggactggc ggcccttgac gacgagcagg
      781 ttgacgcggg tgccctcggg gacgccggtg ccgccgggag ggtcggtctg gaggatggcg
      841 ccgctgggtt cgcccggttc ctcgatttcg gcgatcgtgc cgacatcgag gccgaggttg
      901 cgcagacgga tgccggcggg gattttggtc tcgccgcgca gatcggggac ggtgaccagg
      961 gcctgaccgg aactgacgag gacgcggacg ggggtgccgg ccttgatgcg ggtgccgggg
     1021 gcggggatct ggctgatgac ctggccctcg cgctgggtgg ggtggggctg ttcgccgtcg
     1081 aggacgagca tcaggccgtc ctcgcgcatc gtgcgcaggg cttccaggac gctcaggccg
     1141 gtcaggtcgg gggccaggat ctcctcgccg cggatgtagt gcgccaccac ctggtagctg
     1201 accagaacga tgacggcgaa gaagaggatg atgtagagcc agaagcggat gaattcggcg
     1261 atggtacgga agatccagcg gatcaggcgg aggagccagt ggctgcggcg cgccgcgggg
     1321 agcggggggc ggggggaagg ggcggcggcg agttcagcca tcgaggcggc gacctgcggg
     1381 gacccggcgg cagcgggcgg gttgggggtc ggggcgcggg cgcgtcgggg cgccccgtgc
     1441 cttcagcata atcgcgcggc ggtgggacgc aaccggaaac ggcggggcgg gggaatgacc
     1501 ggcgagggcg ccggtcccgg ggattacgcg gttgagcact cgggggcgtt cgatatttac
     1561 ggtttgttac tcttgatttt aattgcatcg gcctggggcg gggcctaaga tcggttggga
     1621 cttggaagag gaagagtccg aggatcgctc ggatcgggtc ccgaccgcag gccgaacagc
     1681 gctccgccag cgtgcgatag gaaccatcat ggcattcgat cagctttcca gttaccagat
     1741 cgggaagatg ctggatgtat cccgccaggc tgtcaatcaa tggattgaca agggttacat
     1801 tccgtcgtac cggacgccgg gggggcatcg gcgggttcgc cgggaagact tcatcaattt
     1861 tctgaactcg cggcagatcc cgattcccga cattctgcag cgggcgatcg accaggagcg
     1921 ccgttcggcg gcgccgcgga ttattctggt cgatgacgac gcggagtttc tggcggtgct
     1981 gcgccaggcg attcaggagc agttgcccga ggcgcggatt gaggcatacg aaagcggcta
     2041 cgacgcgctg gtcgcgattg gccaggggtt gcccgacctg ctggtgctgg acatcaagat
     2101 gccgaagatc gacggggtgg aagtctgccg gcgattgaaa cgcaacgacg cgacgcggcg
     2161 gctgccggtg atgatcgtct cggcgcacga cacgccggaa tggcgcgagt cgctgcaggc
     2221 gctcaaggtg caggcgatct tctcgaaggg ggaaccgctg ctggagattg cgcggcaaat
     2281 acggcagttt atcgaaacca aagcggcgac gttgaagatc tagcggggga ggggtgaaaa
     2341 cctctgcgca ttcgccgtgg ggccggcgaa agcgattgca caccgactgt tcacaccggg
     2401 cgacaacccc caccagcgcc tgcccggggc gcgacccggc ccaccggccg acccttgatt
     2461 ttgttgaagt ttgaatggag cgagacccgc gggccccccc cgcggggccg gccgtccggc
     2521 cttggcatcg gctttgctga aacctgtcgc cacgagcgtt aagcgcggag ccgagctccc
     2581 gcgagaattc tccgacaggt ggtcaggcga gacgatggcc caacccgatt cggtaactca
     2641 atggcaactg caggtcgtga gcgaacacac cgtccactgc cgccagcgct atttccgcac
     2701 caagacgttg ttcgactggt gcgcggccgc ctccggtctg gtgttgctgg cgccggtgat
     2761 gttcgtgatc gcgctggcgg tgcggctgac gtcgcgcggg ccgattctgt atcgccagat
     2821 ccgcgtcggg atcaataacc agccgtttct gatctacaaa ttccggacga tgcgggtgga
     2881 cgcggagaag gacggggcgc agtgggcgcg gcgcaacgac ccgcgggtga cgccgatcgg
     2941 ccggtttctg cgccggacgc acctggatga actgccgcag ttgatcaacg tgctgaaggg
     3001 ggagatgagc ctggtcgggc cgcggccgga acggccgatc ttcgtcagcg aattgagccg
     3061 gcagatcgag ggctacccgt tgcggctggc ggtcaagccg gggatcacgg gactggccca
     3121 ggtgcaccac cgctacgacg aatcgatcga ggatgtgaag gtgaagctgc attacgatct
     3181 ggcgtatgtc cagtcgtgcg ggttcgggat ggatctgaag atcatctgga acacgttctg
     3241 gtcgctgctg acggggcgga cgcacgacgt gggggcgccg tcgggggcga cgacgggggc
     3301 cgattcggcg aaggcggcgt aagcgcgggc catcactccg aaagatattg tcgatgcttg
     3361 ggatcgattg gggtgggtct gccgccccag cgggactctt tggtctttgg cgttccgttt
     3421 cccgccactg acgcagcggg ctgaagtctg ccgtccctga cgggactggc tggctgatcg
     3481 gtccggcaag gccacagccg ctggaatcgg tcgttcagag ttttccatta tcagctacca
     3541 gatagtcgga gggcgcgagc agcgccggcc acccctttca gggggttcga tgcgttgggg
     3601 ccggcgacgc agggtttcgg cctgtggccg tcaccctgcg cttaaatccg gtcgttcgct
     3661 tcgcggatta gaggattcgc tccttcgggg acggggggaa agggggtgtc cccaggttgc
     3721 gctgtgggca ccacttgggg ttattccaac ggcgccccgc tggggcggct cgcgcggatg
     3781 aaattttcgt ttggagatgc ggttccatct ggcatccctc cgggatgcgg gggaggaggg
     3841 gccgagtga
//
LOCUS       DAOPCO010000351         3839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19314_length_3839_cov_4.813689, whole genome shotgun sequence.
ACCESSION   DAOPCO010000351 DAOPCO010000000
VERSION     DAOPCO010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19314_length_3839_cov_4.813689"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLS90_16115"
     CDS             <1..650
                     /locus_tag="PLS90_16115"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK03973.1"
                     /translation="GVPVMTFDSDVPESARFAFYGVDDIQTGQAVMRELAGQLGGAGR
                     IAILAGNQNAPNLRRRVQGVEQEAANHSGIEIVGVFYHAETPQDAAAEVLRVMNAYPD
                     IDGWAMIGGWPLFTTTLLTDLNPEEVKVVSVDALPAELPYIEAGIAPVLLAQPTYLWG
                     YVSVEKIIDKLMGRDVPEMNRMELVRVTRENLGEWARQLRDWGFEDVPEQYLQME"
     gene            1136..1211
                     /locus_tag="PLS90_16120"
     tRNA            1136..1211
                     /locus_tag="PLS90_16120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1169..1171,aa:Pro,seq:cgg)
     gene            complement(1272..2093)
                     /gene="lpxA"
                     /locus_tag="PLS90_16125"
     CDS             complement(1272..2093)
                     /gene="lpxA"
                     /locus_tag="PLS90_16125"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPK03974.1"
                     /translation="MPTIIHPTAIVDPKAQLGVDVEIGPHSIIEAGVSLGDRTRIGPL
                     VHIQGTTTLGADNQVYTGATLGFPPQYLGFDGSPTRLVIGDRNVIREYVSIHRGLNHE
                     SETRIGDDCFLMATAHVAHDCQIGNRVILVNGVLLAGHVTVGDRANLSGNAVFHQFVR
                     IGAMAMVSGLSWVNKDVPPFALVEGHPARVRGLNSIGMKRAGIAPAARLEIKRVLRQV
                     NAPGRSHRENAAALDLASLCPEARLLAEFYLDSKRGVLPMARAGAETPGDAADAD"
     gene            2455..3159
                     /locus_tag="PLS90_16130"
     CDS             2455..3159
                     /locus_tag="PLS90_16130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPK03975.1"
                     /translation="MKERNLDGKLAVVTGASRGIGRHIARALHAAGATVAITGRNAET
                     LADAARRIGAHCHPYVCDQRSETAVQTMAQQVLAERGTPDILVNNAGVGGGGPVESLS
                     LAEWDRVIGTNLTGVFLTTRAFVPAMKARNRGDIFMISSMSGKKGDPGATAYAASKFG
                     LQGFSQALTYEVRGHNIRVMVLNPSSVDTRENEGPEYGTGLHLHAADLAEMIVHLACL
                     PGRTLFRDMDIWGTNP"
     gene            3184..3492
                     /locus_tag="PLS90_16135"
     CDS             3184..3492
                     /locus_tag="PLS90_16135"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HPK03976.1"
                     /translation="MVNHIVLLKVRANVRPEIVEGLFGELEALRDKIPGITSFTGGPY
                     SGPKGINKGFTHGFIMRFSDAGARDVYMDHPEHEVVKVRLREVLEAGPESLVAFDFEG
                     "
     gene            complement(3594..>3839)
                     /locus_tag="PLS90_16140"
     CDS             complement(3594..>3839)
                     /locus_tag="PLS90_16140"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HPK03977.1"
                     /translation="WRRLRAECRCAFCRDEMTGQRTLDVDAIPADIRPLEISVVGNYG
                     LRILWSDQHGTGIYHFGLLRELAEFTRPVPPDSGNPA"
BASE COUNT          685 a         1127 c         1386 g          641 t
ORIGIN      
        1 gcggcgtgcc ggtgatgacg ttcgacagcg acgtccccga atcggcgcgc ttcgcgtttt
       61 acggcgtcga cgacatccag accggccagg ccgtgatgcg cgaactggct gggcagctcg
      121 gcggcgccgg ccggatcgcg atcctcgccg gcaatcagaa cgcgcccaac ctgcgccggc
      181 gcgtgcaggg ggtcgagcag gaagccgcga accattcggg gattgagatc gtcggcgtgt
      241 tctatcatgc cgaaacgccg caggatgccg ccgccgaggt cctgcgcgtg atgaacgcct
      301 atcccgacat cgacggctgg gcgatgatcg gcgggtggcc gctgttcacc acgacgctgc
      361 tgaccgatct gaatccggag gaggtgaagg tggtctcggt ggacgcgctg ccggccgagc
      421 tgccgtatat cgaggccggc atcgcgccgg tgctgctggc ccagcccacg tacctgtggg
      481 gttacgtgtc ggttgagaag atcatcgaca agctgatggg ccgcgacgtg cccgagatga
      541 acaggatgga actggtgcgc gtgacccggg agaacctggg ggagtgggcg cgccagctgc
      601 gggactgggg atttgaggac gtgccggagc aatacctgca gatggagtaa tgccccctgc
      661 tccagaatcg gcgcttgtcg ctcgtgcgca aaacagtatt tcagacagga cagaaacata
      721 agggacgtta gggacgcatg cagttctgcc gtccctacgg ggactctgtt gctcttgcca
      781 ccgcggttcc cgccactgaa aggagcgggc tgaagtctgc cgcccctgac gggactgctc
      841 cgctgatgaa tccggcaagg gctgggttgt tcgagtcggc cgtgcccccc ttttcatccc
      901 tcgtagtgaa cagcaggttg atgagcaact cgaaatcgcc cccataggcc ctgcgctctg
      961 aggggcgaca ggggagggca tccgctctga aaaattgggc tgatttcgcc gttgcacaag
     1021 cacccggcgg ttgggctata atcctgaatt gctgccctcg ttgcagggca gcgcgggagg
     1081 ggctcgccgg ccgattcagg gccgcccttc cgttcgcggc ggatcgccgt caggtcgggg
     1141 cgtagcgcag ttggtagcgt gcgcggttcg ggaccgcgag gtcgctggtt caagtccagt
     1201 cgccccgacc attttttcca catcaaaaaa aatcaggaaa attgagagag cccgggcgct
     1261 ttggtcgccg gctagtcggc gtcggcggca tcgcccggcg tttcggcgcc ggcgcgcgcc
     1321 atcggcagca cgccccgctt gctgtccagg tagaattcgg ccagcaggcg ggcctcgggg
     1381 cagagcgacg ccaggtccag cgcggcggcg ttctcgcggt gactgcgccc gggggcgttg
     1441 acctgtctca ggacgcgctt gatttcgagc cgcgcggccg gcgcgatgcc cgcccgtttc
     1501 atcccgatgc tgttcagccc gcgcacccgc gccgggtgcc cctcgaccag cgcgaacggg
     1561 ggcacgtcct tgttgaccca cgacagcccg ctgaccatcg ccatcgcgcc gatgcgcacg
     1621 aactggtgaa agaccgcgtt gcccgagaga ttggcgcggt cgccgacggt gacatggccg
     1681 gccagcagga cgccgttgac cagaatcacg cgattgccga tctgacagtc atgcgcgaca
     1741 tgggcggtcg ccatcaggaa gcagtcgtca ccgatgcgcg tttcgctttc gtggttcagg
     1801 ccgcggtgaa tcgagacgta ttcgcggatg acgttgcggt cgccgatgac gaggcgcgtg
     1861 ggcgagccgt cgaagccaag gtattgcggc ggaaagccga gcgtggcgcc ggtgtagacc
     1921 tggttgtcgg cgccgagcgt cgtcgtgccc tggatgtgga ccagcggccc gatgcgggtg
     1981 cggtcgccga gcgagacgcc ggcttcgatg atcgaatggg gcccgatctc gacatcgacg
     2041 ccgagctggg ccttgggatc gacgatggcg gtgggatgaa tgatggtcgg cacgatgttc
     2101 agccttgtgg ctcgcgtggt tgaaggatca gcagcacgcc cggcgaggcg gaaccgcccg
     2161 ccatcggccg cccgccgcgc gccacgggca tcttaatcta ggagtgcaac tggggataag
     2221 caagtccatt gaaccgggcc agggggcgca gacggcttcc cgggccagcg gctccgcggc
     2281 gtcgttgacg cgcggcccgg gccgggcgag aatgcttcaa aacgctcgcc aagtcgttct
     2341 tgtgtctctg atgtccttga tgtccctgcc gatacccgat tcacgacgaa acagacagaa
     2401 gggcgggttt tcgagagccg aggacgaggg acgactttct gactgagggg tggaatgaaa
     2461 gagcggaatc tagacggaaa gctggcggtg gtgacgggcg cgagccgggg aattggccgg
     2521 catatcgccc gggcgctgca cgcggcgggg gcgacggtcg ccatcaccgg ccgcaacgcc
     2581 gagacgctcg ccgacgcggc gcggcgcatc ggcgcccatt gtcatccgta cgtctgcgac
     2641 cagcgctccg aaacggcggt tcagacgatg gcgcagcagg tgctggcgga acggggaacg
     2701 cccgacatcc tggtaaacaa cgccggcgtc ggcggcggcg gaccggtgga aagcctgtcg
     2761 ctcgccgaat gggatcgggt tatcgggacc aacttgacgg gcgtcttcct gacgacgcgc
     2821 gccttcgtgc cggcgatgaa ggcgcgcaat cgcggcgaca tcttcatgat cagttcgatg
     2881 agcggcaaga agggggaccc gggcgcgacg gcgtatgccg cgagcaagtt cgggctgcag
     2941 gggttctccc aggctctgac gtacgaggtg cgcgggcaca acatccgcgt gatggtgctc
     3001 aaccccagca gcgtcgacac gcgcgagaac gaagggccgg aatacggcac ggggctgcat
     3061 ctgcacgccg ccgatctggc cgagatgatc gtgcacctgg cgtgcctgcc cggccgcacg
     3121 ctgttccgcg acatggatat ctggggcacg aacccgtaac gcgagatgaa aggcgaaaga
     3181 gtgatggtca atcacattgt gctgttgaaa gtccgcgcca acgtgcggcc ggagatcgtg
     3241 gagggcctgt tcggtgaact ggaagccttg cgagacaaga ttccgggcat cacgtcgttc
     3301 acgggggggc cgtactccgg tcccaagggg atcaacaagg gttttacgca cgggttcatc
     3361 atgcggttca gcgacgccgg cgcgagggat gtctatatgg accatcccga gcacgaggtg
     3421 gtcaaggtgc ggctgcggga ggtgctggaa gcggggccgg agtcgctggt ggcgtttgat
     3481 ttcgaggggt gagattgaat cgctggaagc gaaggcaaaa gaatgcgctt ttgagaggcg
     3541 aggacgaggg gcgagagacg agagacgaga gacggattct tcagcggtga acttcaggcc
     3601 gggttgccgg agtcgggggg aaccgggcgc gtgaattcgg ccagttcgcg cagcagcccg
     3661 aagtgataga ttccggtgcc gtgctggtcg ctccagagga tgcgcaggcc gtaattgccg
     3721 acgacggaga tctcaagggg tcggatgtcg gcgggaatcg catcgacatc gagcgtgcgc
     3781 tggccggtca tttcgtcgcg acagaacgcg cagcggcatt cggcgcgcag gcggcgcca
//
LOCUS       DAOPCO010000352         3811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19492_length_3811_cov_3.469649, whole genome shotgun sequence.
ACCESSION   DAOPCO010000352 DAOPCO010000000
VERSION     DAOPCO010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19492_length_3811_cov_3.469649"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLS90_16145"
     CDS             complement(<1..920)
                     /locus_tag="PLS90_16145"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03978.1"
                     /translation="MIFIPHARSLAEPLRRVALPLLLLSIYQLILIEFVNPHMAIVWW
                     QLPLLPLLALLARRRRIAPPVAIAYGLIQLYLLSEPAVGWGVVIWFYLPGLLWLVHAC
                     PRPRALLGYGLWTGWMIAMGIYAWLWRAMASFWEIPLLHALPIFALVVVLIGFQMALI
                     FPLVRLAGGRLPFIPIAVGAVIIHAAVEFWMPLPFPIDVALAFSNLPLLLQPADRVGL
                     HGVGLIVLAAGAALAAAVIHARRRRWRRSVLALAALALILSLHLGYGLWAWRRYAPRP
                     SPLSINVALIQPVAPLKIHNADHAAKDRIAR"
     gene            1180..1497
                     /locus_tag="PLS90_16150"
     CDS             1180..1497
                     /locus_tag="PLS90_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPK03979.1"
                     /translation="MLDLKKFQKISKDLQSGMETMKEELAHKTVTGAAGGGVVQVVVN
                     GNQELVSIQIAPEAVDPDDVEMLQDLVLAATNQAIEKSKELQQESINQLTGGMKLPNL
                     PFF"
     gene            1513..2115
                     /gene="recR"
                     /locus_tag="PLS90_16155"
     CDS             1513..2115
                     /gene="recR"
                     /locus_tag="PLS90_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018301636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPK03980.1"
                     /translation="MALLPAALERLLHELTRLPGIGRRGAERIALHLLEAPPEQAGQL
                     AEALAALRREVRPCGLCGNWAEGERCAICADPRRATGQLCVVERAVDIWAFEAAEAYQ
                     GRYHVLGGTLSPLAGVTADDLRITELEDRLGPEGISEVILATNASVDGDATAHYLSQR
                     LSERGLRTTRIAQGVPLGGHLDYADPGTLRLALQGRRPLG"
     gene            complement(2119..3165)
                     /locus_tag="PLS90_16160"
     CDS             complement(2119..3165)
                     /locus_tag="PLS90_16160"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPK03981.1"
                     /translation="MTSPPDSTSSAPPAPLRFVADEAGPRLDRWLAQRLPDRSRNQIQ
                     QDLAAGRVHVNGAAAVARQSVAPGDVILYALPPPSDPRPAAEPIPLTILYEDPHLAVV
                     DKPAGLVVHPAPGHPGGTLVNALLARYGPPLQAVGGDARWGIVHRLDKDTSGLLLVAR
                     TAPAWAALTAAIAQRRVRREYLALVLGRLRDPAGVIDRPLARRERDRKKIGVVPDGRP
                     ARTDWRVLIQGDGLALLALRLHTGRTHQIRVHLQSIGRPVLGDPVYGHTRRRTLMDLP
                     PPLKPVVARLWPDRQLLHAARLVFEHPFLADCRLSITSPPPPDMRRLIAAALPEAEAA
                     IQAWLTTEPPPDAV"
     gene            complement(3178..3498)
                     /locus_tag="PLS90_16165"
     CDS             complement(3178..3498)
                     /locus_tag="PLS90_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPK03982.1"
                     /translation="MTKAVIINKVAQATGMSRKDSIAAVEIFLSSIKDALKDGRKVSL
                     VGFGTFYVKEKNARNGRNPRTGQKIKIPSKSIATFKPGKAFRLQVNDGVDYSQPGDDD
                     DNDV"
BASE COUNT          662 a          994 c         1620 g          535 t
ORIGIN      
        1 cgggcgatcc ggtccttcgc ggcatggtcg gcgttgtgga tcttgagcgg ggcgaccggc
       61 tggatgaggg cgacgttgat cgagaggggc gaggggcggg gggcatagcg gcgccaggcc
      121 cagaggccat agccgagatg cagggagaga atgagggcca gcgcggcgag ggcaaggacg
      181 gagcgacgcc agcggcgccg gcgggcgtga atgacggcgg cggcgagcgc ggccccggcg
      241 gcgagaacga tcaggccgac gccgtgcagg ccgacccgat cggccggttg gaggagaagc
      301 gggaggttgc tgaaggcgag cgcgacatca atggggaagg gcaggggcat ccagaattcg
      361 acggccgcat ggatgatgac ggcgccgacg gcgatgggga tgaacggcag gcggccgccg
      421 gcgaggcgga ccagcggaaa gatgagagcc atctggaagc cgatgagcac gacgactaga
      481 gcaaagatcg gaagggcgtg gaggagagga atttcccaga acgaggccat ggcgcgccag
      541 agccaggcgt agatgcccat ggcgatcatc catccggtcc agaggccgta gccgaggagg
      601 gcgcgggggc gcggacaggc gtgcacgagc cagagaaggc cgggaaggta gaaccagatg
      661 acgacgcccc agccgacggc gggttcgctg agcagataga gctgaatcag gccgtaggcg
      721 atggcgacgg gcggagcgat gcggcggcgg cgggcgagaa gcgccagcag gggaagcagc
      781 gggagttgcc accagacgat ggccatgtgc gggttgacga attcgatgag gatcaactga
      841 taaatggaga gcagcaggag gggaagggcg acgcgccgga ggggttcggc aagggagcgg
      901 gcatggggga tgaatatcat gctgggagga atcgcagggg gtttgggggg tgccggaagt
      961 cgcgggcgga ggatcggttt ggatcatgcg ccccagtcag gatatgcggc ggtgcgtgat
     1021 tggcgagggg aatttttgtt cgagagccga gggacgaggg acgattccgg gtgagatggg
     1081 cggtggcaat tggggaaggg gtggcgtatg ctcgttgggc cgcccgagaa ggggccgcgc
     1141 ggcgggcgac acgcgaaacg ctgacggaga gagtgatcga tgcttgacct gaagaagttt
     1201 cagaaaatca gcaaggactt gcagtccggc atggagacga tgaaggaaga gctggcgcac
     1261 aagacggtga ccggcgcggc gggaggcggc gtggtgcagg tggtcgtcaa cggcaatcag
     1321 gagctggtct cgattcagat cgcgccggag gcggtcgatc cggacgacgt ggagatgctg
     1381 caggacctgg tgctggcggc gacgaaccag gcgattgaga aatcgaagga actgcagcag
     1441 gaaagcatca atcaactgac cggcgggatg aagctgccga atctgccgtt cttctaggat
     1501 tggccgccgc cgatggcgct gttgcccgcc gcattggaac gcctgctgca cgagctgacc
     1561 cggctgccgg ggatcggccg gcgcggggcc gagcgcatcg cgctgcatct gctggaggcg
     1621 ccgccggagc aggcggggca gcttgcggag gcgctggcgg cgctgcgccg cgaggtgcgg
     1681 ccgtgcgggc tgtgcggcaa ctgggcggaa ggggaacggt gcgcgatctg cgccgatccg
     1741 cggcgggcga cgggccagct ttgcgtcgtg gaacgggcgg tcgacatctg ggccttcgag
     1801 gccgccgaag cgtatcaggg acgataccat gtgctggggg ggacgctttc gccgctggcg
     1861 ggggtgacgg ccgacgacct gcgcatcacg gagcttgagg accggctggg gccggagggg
     1921 atcagcgagg tgattctggc gaccaacgcg agcgtggacg gcgacgcgac ggcacattac
     1981 ctgagccagc ggctaagcga gcgggggctg cgcacgacgc ggatcgcgca gggggttccg
     2041 ctggggggcc atctggacta cgccgatccg gggacgctgc ggctggcact gcaggggcgg
     2101 cggccgctgg gctgatgctc agacggcatc cggcggcggt tcggtcgtga gccacgcctg
     2161 gatcgcggcc tcggcttcgg gcagagcggc ggcgatcagg cggcgcatgt cggggggcgg
     2221 cgggctggtg atcgacaggc ggcaatccgc gagaaaggga tgctcgaaga cgaggcgggc
     2281 cgcatggagc agctggcggt cgggccacag gcgcgcgacg accggtttaa gcggcggcgg
     2341 gaggtccatg agcgttcggc ggcgggtgtg accgtagacg ggatcgccga ggacggggcg
     2401 gccgatcgac tggagatgga cgcggatctg gtgggtgcgg ccggtgtgca ggcggagcgc
     2461 gagcagggcc aggccatcgc cctggatcag cacccgccag tcggtgcggg cggggcggcc
     2521 gtcggggacg acgccgattt tcttgcggtc gcgctcgcgc cgcgcgaggg gccgatcgat
     2581 gacgccggcg gggtcgcgca ggcggccgag gacaagcgcg aggtattcgc ggcggacgcg
     2641 gcgctgggcg atggcggcgg tgagggcggc ccatgcgggg gcggtccggg cgacgaggag
     2701 caggccgctg gtatccttgt cgagccggtg gacgatgccc cagcgggcgt cgccgccgac
     2761 cgcctggagc gggggaccgt agcgggccag gagcgcattg acgagcgttc cgccggggtg
     2821 gcccggcgcc gggtggacga ccaggccggc cggcttgtcc acgacggcca gatgtggatc
     2881 ttcatagaga atcgtgagcg ggatcggctc cgccgccggc cgggggtcgg acggcggggg
     2941 gagtgcgtag aggatgacat cgccgggggc gacggactga cgggcgacgg cggcggcgcc
     3001 gttgacgtgg acgcgtccgg cggcaagatc ctgctgaatc tggttgcggc tgcggtcggg
     3061 gaggcgctgg gccagccagc ggtcgagccg cggcccggct tcgtcggcga cgaagcgcag
     3121 gggagcgggg ggcgcggagg atgtcgaatc gggaggggag gtcacgacgc tcggcgatca
     3181 gacatcattg tcatcgtcgt cgccgggctg gctgtaatcg acgccgtcgt tgacctggag
     3241 gcggaaggcc ttgccgggct tgaacgtggc gatggatttg gaggggatct tgattttctg
     3301 gccggtgcgg gggttgcggc cgttgcgggc gttcttctcc ttgacgtaaa acgtgccgaa
     3361 accgacgagg gagaccttcc tgccatcctt gagggcgtcc ttgattgagg aaaggaagat
     3421 ctcgacggcg gcgatgctat ccttgcggct catgccggtg gcctgagcca ccttgttgat
     3481 gatcacggct ttggtcatgc cacgactcct cccgcggatg gcctgggtgg acgcacgggg
     3541 cgccgtcgga ccaaaccggc acctgccatc tcgttgcgca acccgactat tttgaaccct
     3601 tggcgggcag aggtcaagat taacccggcg ggggggaagg gggagtgggt ggaaggcgac
     3661 tgcgggcaag gttgccagtc cccgaagtcg gtggttagga agttgatacg gtttgtggac
     3721 aggattggcg aatttcgaga gttgaggacg agggacgggg ggaggtgttt tttgtgccag
     3781 ggactcaggt tggcgccggg gggttgccga t
//
LOCUS       DAOPCO010000353         3808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19503_length_3808_cov_6.366906, whole genome shotgun sequence.
ACCESSION   DAOPCO010000353 DAOPCO010000000
VERSION     DAOPCO010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19503_length_3808_cov_6.366906"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLS90_16170"
     CDS             <1..663
                     /locus_tag="PLS90_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HPK03983.1"
                     /translation="LKRVVELVMPNLRHYYRLPRKGMVVKSYASDGSYWADVQPLRND
                     DSVDEREPVISRVEIPILWGGPDRGVVCPPAPGTLCDITYYDGDPNYPRISDFRWARN
                     GAPACEVGAFIIQQAPGIFIKITPEGNIVHKTGADLVNETGGDKTETVGGNWGIEVSG
                     DALIKAPQITLDGEVTVTGNLTAGGNISDMGGAKSMSGMRETYNQHTHQGGGAGLPSQ
                     EM"
     gene            667..1101
                     /locus_tag="PLS90_16175"
     CDS             667..1101
                     /locus_tag="PLS90_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HPK03984.1"
                     /translation="MDLFGQDIKLDSNGLALVAATGELILTAGVETGIQDVRLRLGTP
                     LGELFYDIDFGSLIHEFFLDESTQGNRDAFEAEVERRIEEDPRVVLGTVSCSVASWNE
                     KGFTAQAAWQFIDEDHPYNLVISYDSDKKEMVISDVNPRSGL"
     gene            1076..2245
                     /locus_tag="PLS90_16180"
     CDS             1076..2245
                     /locus_tag="PLS90_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014524316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HPK03985.1"
                     /translation="MSIQDLVSRSLDEIRQSLFDRLTSKQEEYAARGWLPIRLNLNKG
                     IVRGLIELWAWGLWQLYQFLGEILKQAFPDTATGKWLDLHCRQVGVSRKAASKAKGVI
                     YFTRLEAGGNVAIPAGRVVKTKPDGAGNVYRYVTTAAAVLAAGTLEVAVPVEAEEFGA
                     VSNATAGQITEIAIVIPGVDAVENRADWLTVEGTDEELDEPLRLRYQLAWKSLNGCTK
                     HAYQAWALEVTGVVDVKILDQHPRGQGTLDVLIVGSAGVPTQQLIDAVAANILGTGND
                     DEKNPINDDVEVRGPTPINIDYAAELELISGDEAAIVAEAENRVRALFAAGPVLAGIS
                     RLGVGQDVTQDRLNWAVMLPTVKKINTDFDDVAVPEDGLAVLHSINITAAWAAEE"
     gene            2247..2888
                     /locus_tag="PLS90_16185"
     CDS             2247..2888
                     /locus_tag="PLS90_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03986.1"
                     /translation="MGRFWDYFRNELRFSPILRSRALALLADGGSETLDSARDAQMQL
                     RDQFLPARCEEAYLIHFARSRGITRHPLEPAEHWLTRVRFAYHWWSRGGRASAMAEAL
                     RSGFNFAAVMVFNHGSEGSLIDETSGVQLVDETSGAILNYERDPSRWAEFAVTIYLAG
                     NEYAYTMDQVIWAINEVKPGRSKLVELFFIAPLHDETSGAGLYDETTGIPLTS"
     gene            2911..>3808
                     /locus_tag="PLS90_16190"
     CDS             2911..>3808
                     /locus_tag="PLS90_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03987.1"
                     /translation="MAGESISWKTYLAGRAPATPPIVDDVFAILQGGTDVKKLRWEDL
                     VRGVANYCEDTGAANALVIASATNYAAYFAGLPFLVKVANTNTGPATLNINNLGAKTI
                     KKNGSAALGAGDLVVGQLIRVAYDGTNFQLLSPPASDLQYAVTTGADNAYAIALDPAP
                     GAYSEGMSVIIKANHTNTGAATLAVGELGAKPIKKHGSAALKANDIRDGQVVYLTYDG
                     TNFQLISSLALDLQYAVATGADNAYEIELTPAADAYLAGMLILFKANFDNTAAATLAI
                     NGMAAKAIKKSGSVALDAGDIKN"
BASE COUNT          812 a         1037 c         1226 g          733 t
ORIGIN      
        1 ctgaagcgcg tggtggagct ggtgatgccg aacctgcgcc actactaccg gctgccgcgc
       61 aagggcatgg tcgtgaagag ctacgcctcg gacggatcgt attgggccga cgtgcagccc
      121 ctgcgcaatg acgacagcgt ggacgaacgc gaaccggtca tttcgcgcgt cgagatcccc
      181 atcctctggg gcggtcccga tcggggagtc gtctgcccgc cggcgccggg aactctgtgc
      241 gacatcacat actatgacgg ggatcccaat tatccgcgga ttagcgattt ccgatgggcg
      301 agaaacggag cgcccgcttg cgaggtggga gccttcatca tccagcaggc gccggggatc
      361 ttcatcaaga tcacgccgga gggaaatatc gtccacaaga cgggcgcgga cctggtcaac
      421 gagaccggcg gtgacaagac tgaaacggtg ggggggaact gggggatcga ggtgtcaggg
      481 gacgcgctga tcaaggcccc ccagatcacg ctggacggag aagtgacggt tacgggcaac
      541 ctgacggccg ggggcaatat ctccgatatg ggcggagcga agagcatgtc tggcatgcga
      601 gagacttata accagcatac ccatcagggc gggggagcgg gactaccctc gcaggagatg
      661 taggaaatgg atctattcgg gcaggacatc aagcttgatt ccaacgggct ggcgctggtg
      721 gcggccaccg gtgagctgat actgacggcg ggcgtggaaa caggcatcca ggacgtgcgc
      781 ctgcgcctgg ggactcccct gggggagctt ttctacgata tcgatttcgg ctccctgatc
      841 cacgagtttt tcctcgatga atccacccag ggcaaccggg acgcgtttga ggccgaggtg
      901 gagcgccgca tcgaggagga cccccgcgtc gtcctcggga cggtttcctg ctcggtggcg
      961 tcctggaatg aaaaaggatt cacggcacag gctgcgtggc agttcatcga tgaggaccat
     1021 ccttacaacc tggtcatcag ttacgacagc gataaaaagg agatggtgat aagcgatgtc
     1081 aatccaagat ctggtctcta ggtcgctgga cgaaatacgt caaagcctgt ttgaccgcct
     1141 gacgagcaaa caagaggaat acgccgcccg gggatggctg ccgatacggc tgaacctgaa
     1201 taagggaatc gtccgcgggt tgatcgaatt gtgggcgtgg gggctctggc agctctatca
     1261 atttctcggg gagatcctga agcaggcatt tcccgatacg gccactggaa aatggctgga
     1321 cctgcactgc cgacaggtgg gcgtgagcag aaaagccgcg agcaaggcta aaggggtcat
     1381 ctattttacc cgccttgagg ccgggggcaa cgtcgctatt ccggccgggc gcgtcgtcaa
     1441 gaccaaaccc gatggggccg gaaacgtcta tagatatgtg acgacggctg ccgctgtatt
     1501 ggctgccgga acgttggaag tggccgtgcc cgtcgaggcg gaggaattcg gggcggtatc
     1561 caacgctaca gccgggcaga tcactgagat cgcaatagtg attcccggtg tcgacgcagt
     1621 ggaaaaccgc gccgactggt tgaccgtgga gggaaccgac gaagagctgg atgagcccct
     1681 ccggctccgt tatcaactgg cgtggaaatc cctgaacggc tgcaccaagc atgcctatca
     1741 ggcgtgggca ctggaggtga ccggcgtggt ggacgtcaag atcctcgatc agcatcctcg
     1801 tggccagggg actctggatg tgctcattgt cggctcagca ggcgtgccga cccagcaact
     1861 catcgatgcg gtggccgcca acatcctcgg caccgggaac gatgatgaaa aaaatccgat
     1921 caatgacgac gtagaggtga gagggccgac tcccatcaat atagactatg cggcggagct
     1981 ggaactgatt tccggcgacg aggcggcaat cgttgccgaa gctgaaaacc gggtgcgtgc
     2041 attattcgcg gccggtcccg tcttggccgg catcagtcgc ctgggcgtcg gccaggatgt
     2101 cacgcaggac cggctcaact gggctgtcat gcttcccacg gtaaagaaga ttaacactga
     2161 ttttgacgat gtggcagttc ccgaggacgg gctggcggtg ctgcatagca ttaacattac
     2221 cgcagcatgg gcggctgagg agtagcatgg gacgtttctg ggattatttc aggaatgaat
     2281 tacggttttc gcctatcctt cggtcgaggg ccttggccct gttggctgac ggcggcagcg
     2341 aaacgcttga ttctgcccgc gatgcccaga tgcaactgcg cgatcaattt ctgccggccc
     2401 gctgtgaaga ggcctacctc atccacttcg cgcggagccg cggcatcact cgtcacccgc
     2461 tggagcccgc ggagcattgg ctgaccagag tgcggttcgc ataccattgg tggtcgcgag
     2521 gcggccgcgc ctccgctatg gccgaggcgt tgcggtcggg gttcaatttt gcggccgtaa
     2581 tggtgttcaa ccatggctcc gaaggatcgt tgatcgatga aacgagcggg gttcaactgg
     2641 ttgatgagac gtctggcgcg atactgaatt acgagcgcga tccgtcgagg tgggcggaat
     2701 tcgcagtgac gatttatctg gccgggaacg agtatgccta caccatggac caggtaattt
     2761 gggcgatcaa tgaggtcaag ccgggtcgct ctaagctggt agagctgttc tttatcgcgc
     2821 ctctgcatga cgaaacgagc ggggccgggt tgtatgatga aacaacgggc ataccgttga
     2881 catcgtaatt ttattgaagg gagattaatt atggccggcg aatcgatatc atggaaaacc
     2941 tacttggcgg gacgtgctcc cgcgacccct ccgatcgttg atgatgtttt cgcgatcctc
     3001 caggggggaa cggatgtgaa aaaactccgg tgggaggacc tcgtgcgcgg ggtggccaat
     3061 tactgcgaag acacgggggc tgccaatgcg ctggtgattg cctctgcaac gaattatgcg
     3121 gcctatttcg cggggctccc gttcttggtg aaggttgcga acaccaatac cgggccggcg
     3181 accctgaaca tcaacaacct gggagccaag accatcaaaa agaacggctc cgccgcctta
     3241 ggggcggggg acctcgttgt cggccagctc attcgtgtgg cctatgacgg gaccaatttt
     3301 cagcttcttt cccctccggc ctcggatctg cagtatgccg tgaccaccgg cgccgacaac
     3361 gcctatgcga tcgccctgga tccagccccc ggcgcttaca gtgagggaat gtcggtcatt
     3421 attaaggcga accataccaa taccggggcg gcgacactgg cggttggaga gctgggggcc
     3481 aagcccatca aaaagcacgg ttcggccgcc ttgaaggcga atgacatcag agatgggcag
     3541 gtggtctacc tgacctatga tgggaccaac ttccagctta tttcctctct ggcgttggat
     3601 ctgcagtacg ccgttgccac cggggctgac aacgcctatg agatcgaact cacccccgcc
     3661 gcggacgcct acctcgccgg gatgctcatc ctctttaaag ccaatttcga caacacggca
     3721 gcggccaccc tggcgatcaa cggcatggcg gccaaggcga tcaaaaagag cggcagcgtt
     3781 gccctggatg ctggagatat taaaaacg
//
LOCUS       DAOPCO010000354         3787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19633_length_3787_cov_5.520632, whole genome shotgun sequence.
ACCESSION   DAOPCO010000354 DAOPCO010000000
VERSION     DAOPCO010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19633_length_3787_cov_5.520632"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLS90_16195"
     CDS             <1..378
                     /locus_tag="PLS90_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03988.1"
                     /translation="IHPHTRGGCMGDGQHIWAAAEWIMLVHNCFLYEEPALNRLVLGA
                     GIASEWLLAGEPLTFGPAPTPWGPVSIRVEPTDERVVVRWQARWRAEPPEIEVRLPEA
                     PPLIAGAGVDHVELARNLAPTLS"
     gene            412..1713
                     /locus_tag="PLS90_16200"
     CDS             412..1713
                     /locus_tag="PLS90_16200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK03989.1"
                     /translation="MNILMMTNTFTPHVGGVARSVSFFTHELRAMGHRVVVVAPEFED
                     MPEQEEDVIRVPAIQNFNGSDFSVIIPVPGYLEAKLEEDFQPDVVHSHHPFLLGSTAL
                     RVASQYQIPLVFTHHTMYEQYTHYVPGDSQRMKVFAVKQATGYCNLCDAVVAPSESVA
                     AILRERGVTVPIQSIPTGVYIEEFQRGDGLRLRRQFGLAPEDRVIGHLGRLAPEKNLP
                     FLARAVSQYLATTDRGHFLVVGGGPSVDEIQAIFDDAGLGDRLHLTGSLSGQDLVDAY
                     HAMDVFTFASKSETQGMVLVEALAAGVPVVALDANGAREVVRDGCNGRLLPHEEEAPF
                     AAALADVVDQLERDPEPLRRGALATAREFAMPRCAERLQALYLQVQRISEFHHMADHS
                     IWRAALEELKAEWDILANLTEAAVAALEEEVEPEEDAGAST"
     gene            1710..>3787
                     /locus_tag="PLS90_16205"
     CDS             1710..>3787
                     /locus_tag="PLS90_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK03990.1"
                     /translation="MIFYLDMFLRRLRRRISRSENLIQLLGLSRNPQGATLPGLVIIQ
                     IDGLSRQQMERAMARKRLPFLRKLLVRQQYKVFSHYSGLPSATPAVQGELFYGIPCAV
                     PAFSFFDRETRRVFRMYDCDDALTIERRLEAEGEPLLKGGASYSNIYSGGADEAHICS
                     TTLGWSGMFSKGSPFALHLFLLFNIYGLLRTVVLLLYEFILAFYDFFRGTIAGQDLWK
                     ELKFVPSRVGVSILSRELVTAGVKLDIARGLPVIHLNLLGYDEQSHRRGPTSAFAHWT
                     LRGIDDAVKRIWLAAQASPCRDYDVWIFSDHGQQEVVPYPVETGETIQEAVERVFDHS
                     LPHRGVGPQERRGIQSQRWRLFNWRFLMRPLIQEGEGRVAEEDHRPIIASMGPVGHIY
                     IQRELGSEQKRSLARRLATEARVPLVLVREGESRVAAFTPQGEFMLPEDGARILGPQH
                     PFLREVVPDLIRLVRHPNAGDFVISGWRRGGKPISFPVENGAHAGPGPDEVEGFVMMP
                     ADTLVSGADKGYLRPVDLHDTAMRLLGRRQPLERCPPVITRDTLRIMTYNVHSCIGMD
                     GKTSPHRVARVIAQYDPDIVCLQELDIGRLRTDGADQAQIIATYLRMDHHFHPALRLE
                     EELYGDAILSRYPMRLVRADALPGLPDRPQLEPRGALWTVVELNGCAIHVLNTHFGLR
                     RREQLCQARAL"
BASE COUNT          597 a         1429 c         1145 g          616 t
ORIGIN      
        1 attcatcccc acacccgcgg cggctgcatg ggcgacggcc agcacatctg ggccgcggcc
       61 gaatggatca tgctcgtcca caactgtttc ctttacgagg agcccgcgct caatcgcctc
      121 gtgctcggcg cgggaattgc ctccgaatgg ctgctggcgg gcgaaccgct cactttcggc
      181 ccggcgccca ccccctgggg cccggtctcg atccgcgtcg agccgaccga cgagagggtc
      241 gtcgtccgct ggcaggcccg ctggcgcgcc gagcccccgg agatcgaagt gcggctgccc
      301 gaggccccgc cgctcatcgc cggcgccggc gtcgatcacg tcgaattggc ccgtaacctg
      361 gcgccaaccc tgagttgaat cgcgaaccct gaccatcgga gcgtccccgg cgtgaacatc
      421 ctcatgatga ccaacacctt taccccccac gtggggggcg tggcgcgctc ggtctcgttc
      481 ttcacccacg agctgcgcgc gatgggccat cgcgtcgtcg tcgtcgcccc cgaattcgag
      541 gacatgcctg aacaggagga agatgtcatc cgcgtccccg cgattcagaa tttcaacggc
      601 agcgatttct ccgtcatcat cccggtcccc ggctacctcg aagccaaact cgaggaggac
      661 ttccagcccg acgtcgtcca ctcgcaccac cctttcctgc tgggcagcac ggcgctgcgc
      721 gtcgcctcgc aatatcagat tcccctcgtc tttacccacc acacgatgta tgagcagtat
      781 acgcactacg tgccgggcga ttcgcagcgc atgaaggttt tcgccgtcaa acaggccacc
      841 ggctactgca acctgtgcga cgccgtcgtg gcccccagcg aaagtgtggc cgccatcctg
      901 cgcgaacgcg gcgtgacggt cccgatccag agcatcccga cgggcgtcta catcgaggaa
      961 ttccagcgcg gcgacggctt gcgcctccgc cgccaattcg gcctggcgcc cgaagaccgc
     1021 gtgatcggcc acctcggccg gctggcgccc gaaaaaaacc tgccctttct ggcgcgggcc
     1081 gtcagccaat atctcgcgac aaccgaccgc ggccactttc tcgtcgtcgg cggcgggccc
     1141 tcggtcgacg aaatccaggc gatcttcgac gatgccggcc tcggcgaccg gctccacctg
     1201 accggcagcc tcagcggcca ggatctcgtc gacgcctacc acgcgatgga cgtgttcacc
     1261 ttcgcctcca agagcgaaac gcaggggatg gtcctcgtcg aggcgcttgc cgccggcgtg
     1321 ccggtcgtcg ccctggacgc caacggcgcg cgcgaagtcg tccgcgacgg ctgcaacggc
     1381 cgcctgctgc cgcacgagga ggaggctccc ttcgcggcgg cgctggccga cgtcgtcgat
     1441 caactcgagc gcgaccccga gccgctgcgc cgcggcgccc tcgccaccgc ccgggagttc
     1501 gccatgcccc gctgcgccga acgcctccag gcgctgtatc tgcaggtcca gcgcatcagc
     1561 gagttccacc acatggccga ccacagcatc tggcgcgccg ccctggagga actcaaggcc
     1621 gaatgggaca tcctggccaa tctgaccgaa gcggccgtcg ccgccctcga ggaagaagtc
     1681 gaaccggagg aggatgcggg cgcgagcaca tgatcttcta tctggacatg ttcctgcgac
     1741 gcctgcggcg tcggatcagc cgcagcgaaa atctgatcca actgctcggc ctatcgcgca
     1801 acccccaggg cgcgaccctc cccggcctgg tcatcatcca gatcgacggg ctcagccgcc
     1861 agcagatgga acgggcgatg gcccgcaagc gcctcccctt cctgcgcaag ctcctcgtcc
     1921 gtcagcaata caaggtcttc tcgcattact ccggtctgcc ctcggcgacg ccggccgtgc
     1981 agggcgaact gttctacggc atcccctgcg ccgtccccgc gttcagcttc ttcgaccgcg
     2041 agacgcggcg cgtctttcgc atgtatgact gcgacgacgc gctgacgatc gagcgccgcc
     2101 tcgaggccga gggcgaaccg ctgctcaagg ggggcgcctc gtactccaac atctactcgg
     2161 gcggcgcgga cgaagcgcat atctgctcca ccacgctcgg ctggagcggc atgttctcca
     2221 agggttcccc cttcgccctg catctcttcc tgctgttcaa tatctacggc ctgttgcgca
     2281 ccgtggtcct gctgctctac gaattcatcc tggcgttcta tgattttttc cgcggcacga
     2341 tcgccggcca ggatctgtgg aaggaactca agttcgtccc ctcgcgggtg ggcgtctcga
     2401 tcctctcgcg cgaactggtc accgccggcg tcaagctcga catcgcgcgc ggcctgccgg
     2461 tgattcacct caatctgctc ggctacgacg agcagtcgca tcgccgcggg ccgacctccg
     2521 ccttcgccca ctggacgctg cgcggcatcg acgacgccgt caagcgcatc tggctcgccg
     2581 cccaggcctc cccctgccgc gattacgacg tctggatttt ctccgaccac ggccagcagg
     2641 aggtcgtccc ctacccggtt gaaaccggcg agacgatcca ggaggccgtc gagcgggtct
     2701 tcgaccactc gctgccgcat cgcggcgtcg gcccccagga gcgccgcggc atccagtccc
     2761 agcgctggcg gctgttcaac tggcgcttcc tcatgcgccc gctgatccag gagggcgagg
     2821 gccgcgtcgc cgaggaggat caccgcccga tcatcgcgtc gatgggtccg gtcggccaca
     2881 tctacatcca gcgcgagctc ggcagcgaac agaagcgcag cctggcgcgg cggctggcga
     2941 cggaggcccg cgttccgctc gtcctagtcc gcgaggggga gtcgcgcgtc gccgcgttca
     3001 ccccccaggg cgagttcatg ctgcccgagg atggcgcgcg catcctcgga ccccagcatc
     3061 cgttcctgcg cgaggtcgtt cccgatctga tccggctcgt gcgccatccc aacgccggcg
     3121 atttcgtcat cagcggctgg cgtcgcggcg gcaaaccgat cagcttcccc gtcgaaaacg
     3181 gcgcccacgc cggccccggt cccgacgagg tcgaggggtt cgtcatgatg cccgccgata
     3241 cgctcgtcag cggcgccgac aagggctacc tgcggcccgt cgacctgcac gataccgcga
     3301 tgcgcctgct cggccggcgc cagccgctcg agcgctgccc ccccgtcatc acccgcgaca
     3361 cgctgcgcat catgacctac aacgtccata gctgcatcgg catggacggc aaaacgtctc
     3421 cgcatcgcgt cgcccgcgtc atcgcgcagt acgatcccga catcgtctgc ctgcaggaac
     3481 tcgacatcgg ccggctgcgc accgacggcg ccgaccaggc ccagatcatc gccacctacc
     3541 tgcgcatgga tcatcatttc caccccgcgc tgcggctgga ggaggagctc tacggcgacg
     3601 cgatcctgag ccgctacccg atgcggctcg tccgcgccga cgccctgccg ggcctgcccg
     3661 accggcccca actcgaaccg cgcggcgcgc tgtggaccgt cgtcgagctc aacggctgcg
     3721 cgatccacgt cctgaacacg catttcggcc tgcgccggcg cgaacagctc tgccaggccc
     3781 gcgcgct
//
LOCUS       DAOPCO010000355         3766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19768_length_3766_cov_3.524926, whole genome shotgun sequence.
ACCESSION   DAOPCO010000355 DAOPCO010000000
VERSION     DAOPCO010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19768_length_3766_cov_3.524926"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="PLS90_16210"
     CDS             <1..1275
                     /locus_tag="PLS90_16210"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPK03991.1"
                     /translation="ARYYPTNDLVTAHEIIFFWVARMIMMGLKFMGDVPFRQVVINPL
                     VMDEHGKKMSKSSGTAIDPLEVVEEIGADSIRLALASYPTQSRHISLSEKRFESMRNF
                     CNKLWNAARFVLMNTEDLPAEGFAELDRNPALAWEDHWLLGALAQAIARADQALESYA
                     FDGYVDAVYKFTWNQYCDWYVELVKDRLHGKAPAGSVRNRPESRRAAQAVLVVALETT
                     LRLLHPVAPFITEEIWGKIRARWGEGLELGAEGLMRTRWPEAGRFQQDASAAQAMELV
                     QEAISRIRNIRGEMNVAPGVAVDLELVSAEPARLEALRHGEPYFRSLVTVGSVGFGAV
                     RQRAGFAATSVIGDVTVHVYLPEELREREKGRLEKEIARLEKGVRGAEGKLGNEKFLT
                     GAPAEVVEAERERLERLRSELETIRAKRAALG"
     gene            1480..2799
                     /locus_tag="PLS90_16215"
     CDS             1480..2799
                     /locus_tag="PLS90_16215"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPK03992.1"
                     /translation="MAQAAPGATTRNDLTIRFEGDSGDGILSLGMIAARTASRSGLHV
                     YTYSSYLAVVRGGQSAMQVRIGRQPLLSQGDRPDVLVALNYQAVRNHAGHVAPGGLIL
                     HPHFVEPIADLVNEGVRCIEVNFREVALEETGVPRGMNLVAAGAMLQLLGLEEQVAVG
                     VVREQFAKKGPEVAELNVRALQAGYQTTAGVNGQAADLRLKSVDGRPRPLLSGNEAVA
                     FGALVAGVRFFAGYPITPATEILEWLAHQLPRVGGRIVQAEDEIAALAMCIGAAFGGA
                     KTMTATSGPGLSLMTELIGLAGMAELPIVIVDVQRAGPSTGMPTKEGQGDLRLAVHGT
                     HGEVPRVVLAPQTVEDCFRDTIRAVGIAHQFHPGDHPVEPVAGSADADDRPAGPGGDP
                     GLRGTVPSGAGGRGIRAVRADGRWARLAADDPGDAGGDVSHGGSGAR"
     gene            2768..3328
                     /locus_tag="PLS90_16220"
     CDS             2768..3328
                     /locus_tag="PLS90_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03993.1"
                     /translation="MYRTGGLEHDESGNPSYEPEVRNRMVARRRQRMDAIEREFRCGL
                     IRDGYRNGRCPLGVVAWGSTASVVQEVVESLQREEWKIARFFPHILWPMPDTAFEEFL
                     DSGIRTLFVCELNSTRQFAEMIQARYTARLIEHKIRVISITKDDGLPFTPEGIREGML
                     HHLSEKILRTEGLPFSPRQFKEMIFG"
     gene            3380..>3766
                     /locus_tag="PLS90_16225"
     CDS             3380..>3766
                     /locus_tag="PLS90_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK03994.1"
                     /translation="MKPAAIEPAEETPRRTRSYKSKYKPTWCAGCGDYSVLAALDKAL
                     MILGLPEHEIAIVSGIGCSSRFPFFMDTYGIHTAHGRALSVATGLKSVRPELTVIATG
                     GDGDALAIGGNHFFHTMRRNVDLTYLL"
BASE COUNT          641 a         1000 c         1525 g          600 t
ORIGIN      
        1 gcgcgctact atccgacgaa cgacctggtg acggcgcatg agatcatctt cttctgggtc
       61 gcgcggatga tcatgatggg cctgaagttc atgggcgacg tgccgttccg gcaggtggtg
      121 atcaatccgc tggtgatgga cgagcacggc aagaagatga gcaagtcgag cgggacggcg
      181 atcgacccgc tggaggtggt cgaggagatc ggcgccgatt cgatccggct ggcgctggcc
      241 tcctatccga cgcagagccg gcacatcagc ctgagcgaga agcggttcga atcgatgcgg
      301 aatttctgca acaagttgtg gaacgccgcg cgcttcgtgc tgatgaacac cgaggacctg
      361 ccggccgagg ggttcgccga gctggaccgg aatccggcgc tggcgtggga ggaccactgg
      421 cttctggggg cgctggcgca ggcgatcgcg cgggcggatc aggcgctgga aagctacgca
      481 ttcgacggat atgtcgatgc cgtctacaag ttcacgtgga atcagtactg cgactggtat
      541 gtggagctgg tgaaggaccg gctgcacggc aaggcgccgg cgggatcggt gcgcaatcgg
      601 ccggaatcgc ggcgggcggc gcaggcagtg ctggtggtgg cgctggaaac gacgctgcgg
      661 ctgctgcatc cggtggcgcc gttcatcacc gaggaaatct ggggaaaaat ccgcgcccgc
      721 tggggggagg ggctggagct gggcgcggag gggctgatgc ggacgcgctg gccggaagcg
      781 gggcggttcc agcaggacgc atcggccgcg caggcgatgg aactggtgca ggaggcgatc
      841 agccggatcc gcaatattcg gggcgagatg aacgtcgcgc cgggggtggc ggttgatctg
      901 gaactggtct cggcggaacc ggcgcggctg gaggcgctgc gccacggcga gccgtatttc
      961 cgcagcctgg tgacggtggg gagcgtgggg ttcggagcgg tgcgccagcg cgcggggttt
     1021 gccgcgacga gcgtgatcgg cgacgtgacg gttcacgtat atctgccgga ggaactgcgc
     1081 gaacgggaga aggggcggct ggagaaggag atcgcacgcc tggagaaagg ggtgcgcggc
     1141 gccgagggga agctgggcaa cgagaagttc ctgaccgggg cgccggccga ggtggtcgag
     1201 gccgagcggg agcggctgga gcggctgcgc tcggagctgg agacgatccg ggcgaagcgg
     1261 gcggcgctgg ggtgaggggc taaggctgga gtcgggggag atgggggacg aagtggacga
     1321 gggacgactt tggtgagagg cgacgggatt tgcgattttg gggtacgaaa ggcttgcaag
     1381 ggcgggggat ccgtggtatg aatccatact tgtggatctg tatatcacct aatgtgaacg
     1441 gtcgccgaat ccatttcaca atcccaggag acgactccga tggcccaggc cgcaccgggc
     1501 gcgacgacgc gcaatgacct gacgatccga ttcgaggggg attccggcga cgggatcctc
     1561 tcgctgggca tgatcgccgc ccgcaccgcc tcgcgcagcg ggctgcacgt ttacacttac
     1621 agcagctacc tggcggtggt gcggggcggg cagtcggcga tgcaggtccg gatcgggcgg
     1681 caaccgctgc tgtcgcaggg cgaccggccc gatgtgctgg tggcgctgaa ttaccaggcg
     1741 gtgcgcaacc acgcggggca tgtggcgccg gggggactga tcctccaccc gcatttcgtg
     1801 gagccgatcg cggatctggt gaacgagggg gtgcggtgca tcgaggtcaa cttccgcgag
     1861 gtggcgctgg aggagacggg ggttccgcgc gggatgaacc tggtggcggc cggggcgatg
     1921 ctgcaactgc tgggccttga ggagcaggtg gccgtgggcg tcgtgcgcga gcagttcgcg
     1981 aagaaggggc ccgaggtggc ggagctgaac gtgcgggcgc tgcaggcggg gtatcagacc
     2041 accgccgggg tgaacgggca ggcggcggat ttgcggctga agtcggtcga cggccggccg
     2101 cggccgttgc tgtcgggcaa cgaggcggtg gcgttcgggg cgctggtggc gggggtgcgg
     2161 tttttcgccg ggtatccgat cacgccggcg acggagattc tggagtggct ggcgcatcag
     2221 ttgccgcggg taggtggtcg gatcgtgcag gcggaggatg aaatcgcggc gctggcgatg
     2281 tgcatcgggg cggcgttcgg cggggcgaag acgatgaccg cgacgagcgg gccggggctg
     2341 tcgctgatga ccgaactgat cgggctggcg gggatggcgg agctgccgat cgtgatcgtc
     2401 gacgtgcagc gggccgggcc gtcgaccggg atgccgacga aggaagggca gggggacctg
     2461 cggctggcgg tgcacgggac gcatggcgaa gtgccgcggg tggtgctggc gccgcagacg
     2521 gtcgaggatt gtttccgcga cacgatccgg gcggtcggga tcgcgcacca gttccacccc
     2581 ggtgatcatc ctgtcgagcc agtcgctggg tcagcggatg cagacgatcg acccgccgga
     2641 cctggaggcg atcccggtct acgaggaacc gttccatccg gtgcgggagg gagaggaatt
     2701 cgtgcggttc gcgccgacgg aagatgggca cgcctcgccg cggacgatcc cggggatgcc
     2761 gggggggatg tatcgcacgg ggggtctgga gcacgatgaa tcgggcaatc cgagctacga
     2821 gccggaggtg cgcaaccgga tggtggcccg gcgccggcag cggatggacg cgattgaacg
     2881 ggagttccgt tgcgggctga tccgcgacgg ctaccgcaac gggcgctgtc cgttgggggt
     2941 cgtggcgtgg gggagcacgg cctcggtggt gcaggaggtg gtcgagtcgc tgcagcgcga
     3001 ggagtggaag atcgcgcgat tcttcccgca catcctgtgg ccgatgcccg atacggcgtt
     3061 cgaggaattt ctcgattcgg ggatccggac gctgttcgtc tgcgagttga actcgacgcg
     3121 gcagtttgcc gagatgattc aggcgcgcta cacagcccgg ttgatcgagc acaagatccg
     3181 ggtgatcagc atcacgaagg acgacgggct gccgttcacg ccggagggga tccgcgaggg
     3241 gatgctgcac cacttgagcg agaagatcct gcggaccgaa gggctgccgt tcagcccccg
     3301 gcagttcaag gaaatgatct ttggttgaag acacaccagg gagcgggaca ggatgaacaa
     3361 cccgaacggc aatggtcgga tgaagccggc ggcgatcgag ccggccgagg agacgccccg
     3421 gcggacgcgg agctacaagt cgaagtacaa gccgacatgg tgcgccgggt gcggggatta
     3481 cagcgtgctg gccgcgctgg acaaggcgct gatgatcctc gggctgcccg agcacgagat
     3541 cgcgatcgtt tcggggatcg gctgttccag ccggttcccg tttttcatgg atacgtatgg
     3601 gattcacacg gcgcatgggc gggcgctgtc ggtggcgacg ggactcaagt cggtgcggcc
     3661 cgagctgacg gtgatcgcga cgggtggcga cggcgacgcc ctggcgatcg gggggaacca
     3721 tttttttcat acgatgcggc gtaatgtcga cctgacctac ctgctg
//
LOCUS       DAOPCO010000356         3762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19789_length_3762_cov_4.301592, whole genome shotgun sequence.
ACCESSION   DAOPCO010000356 DAOPCO010000000
VERSION     DAOPCO010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19789_length_3762_cov_4.301592"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PLS90_16230"
     CDS             <1..190
                     /locus_tag="PLS90_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK03995.1"
                     /translation="AWGASGRWFKSSRPDHFFFHFSAILQFSASLTVPGSWFGTVGGE
                     KCGSGASISVLNSLSFYM"
     gene            789..1634
                     /locus_tag="PLS90_16235"
     CDS             789..1634
                     /locus_tag="PLS90_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK03996.1"
                     /translation="MGRFSRLETQPSARKADAAPGEFGGGGDYPPSEEAPPGDAGLDA
                     PGCVQRGWRALAREDYRSALRWFSRALDLDSRRPEPWIDVALTHLLLGDHKQAQTWIG
                     RGLIAFPEEPGLMALRAVCHARRGLLRQAMNHSDAVLERSPNLALAHVARGEVLMLAD
                     NPNQDYCFEQALKTAGGSDWMTPLVICLILRGRKQWVKALTYAIAATERNEREPALWY
                     HVGRLRAALGQGAKAERAFEQARALCAADDPLRPKLERKADGFLLTRLKAWFGPKGTK
                     AGKSG"
     gene            complement(1642..3462)
                     /locus_tag="PLS90_16240"
     CDS             complement(1642..3462)
                     /locus_tag="PLS90_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HPK03997.1"
                     /translation="MPYQPIENYAVIGDLHTVALVGMDGSIDFMAFPFFDSPTIFAAI
                     LDDERGGRFRLAPVLEQARQKQLYLPDSNVLLSRFLSREGVAEISDFMPIEPVAGRQH
                     HALVRRAKTVRGDFRYRMVCRPRFDYARAPHRVETRPGEVLFITEQPAPMALRLRLDV
                     PFRIENGDVVAEFHRAAGQTASFILEQVIPERDSPSAHPDYVAEQFKATVNFWRRWIG
                     RCAYRGRWREVVNRSALMLKLLTSAPHGSLVAAPTFGLPEAIGGRRNWDYRYTWIRDA
                     SFTLYALIRLGFTDETHAFMGWIQQRCKAAADGEGLQIIYGIDGRRDLPEVELQHLDG
                     YRGSQPVRIGNGAWNQLQLDIYGELMDSVYLYNKYGQPIDYDLWVSLVRLIHWVCDNW
                     RRPDQGIWEVRGGAHAFLYSRLMCWVALDRAVRLALKRSFPAPLEKWIAVRDEIYLEI
                     MTHYWNPERHAFVQTRGGSAMDASTLLMPMVKFISPTDPRWLSTLRCIERDLVDDSLV
                     FRYRLNEGVADGFLSGEGTFTMCSFWYVECLSRSGDIEKARLVLEKALGYANHVGLYA
                     EELGPRGEHLGNYPQAFTHLGLISAAFDLNRRLDRAGQAG"
BASE COUNT          630 a          981 c         1527 g          624 t
ORIGIN      
        1 cgcttggggt gcgagtggtc gctggttcaa atccagtcgc cccgaccatt ttttcttcca
       61 tttttcagca attttgcagt tttcggcgag tttgacggtt ccgggttcgt ggtttggaac
      121 cgttgggggg gagaaatgcg gaagcggcgc ctctatttct gtcttaaata gtttatcgtt
      181 ttatatgtaa ttcggttatt ttgatccaag cattgacgtt gtggacaggg atggacgggg
      241 ttcgggtcgg ggcggtgacg cagggttccg gcctgcggcc tcgaccctgc ccatgttccg
      301 gacgtccgct tcgcggactc gagggttcgc ccccctatgg ggacgggatg ttttgggatc
      361 gacgttcccc aggtttcgct gcgcttcatc cggggccatt caaagggtgc ccctgcgggg
      421 cgaggggtgg catccctccg ggatgcgggg aatgtggtgg ctgggtgccc ggtggtgtcg
      481 ctgcgctcaa ccaccggcta cttgctggca tccctgcggg atgcgatgcg ggacagggac
      541 ctacagggac ggacacgggc aacaggcagc ggtgaagggg tttggagcgg cgaggggcgc
      601 gggcccacat cgcggcggtc tcccacggag ccggtcccac gacgcggcgg ccgcacaccg
      661 tgcaaggcca atcgcgtggg cagattcgca tttggttttg gcggggcgtt ccaatatggt
      721 gaaggggtca ggtcggcgcg agcgggtgcg gcgccggatg gagcgctcag ccggggaagg
      781 tcagcgcgat gggccggttt tcacgcctcg agacccaacc ttcggcgcgc aaggctgatg
      841 cggcaccggg ggaattcggc ggcggggggg attatccgcc gagtgaggag gccccgccgg
      901 gcgacgcggg gctggatgcg ccgggctgcg tgcagcgggg gtggcgggcg ctcgcgcgcg
      961 aggattaccg gtcggcgctg cgctggtttt cgcgggcgct ggatctggac tcgcggcggc
     1021 ccgagccgtg gatcgacgtg gcgctgacgc atctgctgct gggcgatcac aagcaggcgc
     1081 agacgtggat cgggcggggg ctgatcgcgt ttcccgagga gccggggctg atggcgctgc
     1141 gggcggtgtg ccatgcgcgc cgggggctgc tgcgccaggc gatgaaccat tccgacgcgg
     1201 tgctggagcg gagcccgaat ctggcgctgg cgcacgtggc gcgcggcgaa gtgctgatgc
     1261 tggccgacaa ccccaaccag gattactgtt tcgagcaggc gctgaagacg gccggtggga
     1321 gcgactggat gacgccgctg gtgatctgcc tgatcctgcg cgggcggaaa cagtgggtca
     1381 aggcgctgac gtatgcgatt gcggcgaccg agcgcaacga gcgtgaaccg gcgctgtggt
     1441 atcacgtggg gcggttgcgg gcggcgctgg ggcagggggc gaaggcggag cgggcgttcg
     1501 agcaggcgcg ggcgctgtgc gcggcggacg atccgctgcg gccgaaactg gagcgcaagg
     1561 cggacgggtt cctgctgacg cggctgaagg cgtggttcgg gccgaagggg acgaaggcag
     1621 ggaaatcggg ttgagggggt ctcagccggc ctggccggcg cggtcgaggc ggcgattgag
     1681 gtcgaaggcg gcgctgatca ggccgaggtg ggtgaaggcc tggggataat tgcccagatg
     1741 ctcgccgcgc gggccgagtt cctcggcata gaggccgacg tgattggcat agccgagggc
     1801 tttttccaga acgagccggg ccttttcgat gtcgcccgaa cgcgagagac attcgacgta
     1861 ccagaacgag cacatggtga aggtcccttc gccgctgaga aagccatcag cgaccccctc
     1921 gttgaggcga tagcggaaga cgagcgagtc atcgacgaga tcgcgctcga tgcaacggag
     1981 ggtggaaagc cagcgggggt cggtggggct gatgaacttg accatgggca tgagcagggt
     2041 ggaggcatcc atcgccgagc cgccgcgggt ctggacgaag gcgtggcgct cgggattcca
     2101 gtagtgggtc atgatttcga ggtaaatttc gtcgcggacg gcgatccact tctcgagcgg
     2161 ggcggggaag gaacgcttga gggcgaggcg gacggcgcga tcgagcgcga cccagcacat
     2221 gaggcgagaa tagaggaacg cgtgggcgcc gccgcggacc tcccagatgc cctgatcggg
     2281 acggcgccag ttgtcgcaga cccagtggat gaggcgcacg aggctgaccc agaggtcgta
     2341 atcgatcggc tggccgtatt tattgtagag gtagaccgag tccatcagtt cgccgtagat
     2401 atcgagctga agctgattcc aggcgccgtt gccgatgcga acgggttggg agccgcgata
     2461 gccatcgagg tgctggagtt cgacttcggg cagatcgcgg cggccgtcga tgccgtagat
     2521 gatctgaagt ccctcgccgt cggcggcggc cttgcagcgc tgctggatcc agcccatgaa
     2581 ggcgtgggtt tcgtcggtga agccgaggcg gatgagggca taaagggtga aggaggcgtc
     2641 gcggatccag gtgtagcggt agtcccagtt gcggcggccg ccaatggcct cgggcagacc
     2701 gaaggtgggg gcggcgacga gcgagccgtg gggggcggag gtgaggagct tgagcatgag
     2761 ggcgctgcgg ttgacgacct cgcgccagcg gccgcggtag gcgcagcgtc cgatccagcg
     2821 gcgccagaaa ttgacggtgg ccttgaactg ctcggcgacg tagtcggggt gggcggaggg
     2881 ggagtcgcgt tcggggatga cctgttcgag gatgaaggaa gcggtctggc cggccgcgcg
     2941 gtggaattcg gcgacgacgt cgccgttctc aatgcggaag gggacgtcga ggcggaggcg
     3001 gagggccatc ggggcgggtt gctcggtgat gaacaggacc tcgcccgggc gggtctcgac
     3061 gcgatgcggg gcgcgggcgt agtcgaagcg cgggcggcag accatccggt agcggaagtc
     3121 gccgcgaacg gtcttggcgc ggcggacgag ggcgtggtgc tgccggccgg cgacgggctc
     3181 gatgggcatg aagtcggaga tttcggcgac tccctcgcgc gagaggaagc gcgagaggag
     3241 gacgttggaa tcgggaaggt agagttgctt ctggcgcgct tgctcgagga cgggggcgag
     3301 tcggaagcgg ccaccgcgct cgtcgtcgag gatcgcggcg aagatcgtgg gcgaatcgaa
     3361 gaaggggaag gccatgaagt cgatcgagcc atccatgccg acgagggcga cggtgtggag
     3421 gtcgccgatg acggcgtagt tttcgatcgg ctggtagggc atggggcgcg actccgcaag
     3481 aagagaggaa gcaagagggg tgccgtgcgg ggcggggccg tggccggagc gttgggggac
     3541 ttgagggaag gccggagcgg ggcgggcggc gggggacgcc atccggacat cccccggaat
     3601 tcatttttgg ttcaacatgc catcagatca cgttggattc atcgcggcgg cgcagctcga
     3661 tcttgcgctt gcgcaggtcg agatcccaga tggagcggtg ggtggcgagg acgaccacga
     3721 agagcatgaa cgcatagagc aggagagccc aggggaggag cg
//
LOCUS       DAOPCO010000357         3750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19867_length_3750_cov_4.423816, whole genome shotgun sequence.
ACCESSION   DAOPCO010000357 DAOPCO010000000
VERSION     DAOPCO010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19867_length_3750_cov_4.423816"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /gene="thpR"
                     /locus_tag="PLS90_16245"
     CDS             <1..488
                     /gene="thpR"
                     /locus_tag="PLS90_16245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPK03998.1"
                     /translation="PEAIHLTLVFLGSQPAGAVAPVAAALERLGRMYAPLRIELKRLG
                     VFPHWRGPRVLWVGVRDRTHQFEGLRRELELSLAPVGYEPDGRPFRPHLTLARFKSPK
                     GSPAVKKVVETHQEFLFGPFVAGELVLFQSELKPEGAVHTALHRTALVAPAPNRNEVE
                     P"
     gene            485..1048
                     /locus_tag="PLS90_16250"
     CDS             485..1048
                     /locus_tag="PLS90_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPK03999.1"
                     /translation="MKQQPVICCRALWHTPIGPMAGSASTRGIVELRFPPADQLVTPA
                     DLIGEVERIDYEEHAEGAPAAARAHLAALEDYLQAYFQGEEAPAPPLDETGLSEFTRR
                     VWRAARAVPAGETTTYRELARRAGSPAACRAVGGVMAANRAVLLMPCHRVLPTGGRAE
                     APGQFGAGTAMKSRLLRHERRLRAISV"
     gene            1718..2257
                     /locus_tag="PLS90_16255"
     CDS             1718..2257
                     /locus_tag="PLS90_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK04000.1"
                     /translation="MKNYLGEIIYASRQKDNLTQDQYGARYNVSGPAIFKFEKGYVKP
                     SLELWLKMAADAAISERRAVLLWVKSKLPEEYQEYIELQSAAVVESEAESVKKKGKKP
                     DYSKLENREQMREFLDKDKAMPKGLRDLLKDDELWALYKPTGHEINMLRDIFGPLGRG
                     SKTAYREALRLIREFAHSF"
     gene            2483..3301
                     /locus_tag="PLS90_16260"
     CDS             2483..3301
                     /locus_tag="PLS90_16260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK04001.1"
                     /translation="MKPGFPILMYHALWPAGLSPAEVDAHCRRDPQLADPGARRYALD
                     AAVFDRQLEQLARHAAPMAAQWDDLARGRGAPRQAWITFDDGHASNHELALPLLRRRG
                     LRAAFFITTDWIGAERFMTPAMLRALRDAGMLLGSHGCSHRYLSDLTTAELDRELRES
                     KARLEELLSEEIPALALPGGRGDGRVRERARAAGYRYLFTSRVGLAPPEGDPLALPRV
                     PIVNDLPEEFLSRLLAGETAELARMARGARRREFARRMLGNRIYDRLRGWVMRE"
     gene            complement(3433..>3750)
                     /locus_tag="PLS90_16265"
     CDS             complement(3433..>3750)
                     /locus_tag="PLS90_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase"
                     /protein_id="HPK04002.1"
                     /translation="VAEAACVGRAHDVKGQALVAFVTMKESAEVSDNVFEKLKEHVAN
                     KIGAIARPDEIILTADLPKTRSGKIMRRLLKDIAEGRMLGDTTTLADPAVVSMLQRQY
                     SEE"
BASE COUNT          609 a         1113 c         1368 g          660 t
ORIGIN      
        1 agccggaggc gatacacctg acgctggtgt ttctggggag ccagccggcg ggggccgtcg
       61 cgccggtcgc ggcggccctg gagcgactcg gccggatgta tgcgcccctg cggatcgagc
      121 tgaagcggct gggggttttt ccccactggc gcgggccgcg cgtgctgtgg gtcggcgtgc
      181 gcgaccggac ccatcagttt gaggggctgc gccgtgaact cgagttgtcg ctggcgccgg
      241 tgggttatga gcccgacggc cggccgttcc ggccccatct gacgctggcg cggttcaagt
      301 cgccgaaagg ctcgccggcg gtgaagaagg tggttgagac gcaccaggag tttctgttcg
      361 ggccgttcgt cgccggcgaa ctggtcctgt ttcagagcga gctgaaaccc gaaggggccg
      421 tgcatacggc gctgcaccgg acggcgctgg tcgcaccggc gcccaatcga aacgaggtgg
      481 aaccatgaaa cagcaaccgg tgatctgctg ccgggcgctc tggcatacgc cgatcggtcc
      541 gatggccggg tcggccagca cgcgcggcat cgtggaactg cggttcccgc cggcggacca
      601 gcttgtgacg ccggcggacc tgatcgggga agtggagcgg atcgattacg aggaacacgc
      661 cgagggggcg ccggcggcgg cgcgtgcgca cctggcggcg ctggaggact acctgcaggc
      721 gtattttcag ggggaggagg cgccggcgcc gccgctggat gaaacgggtt tatccgagtt
      781 cacgcggcgg gtgtggcggg cggcgcgcgc cgtgccggcc ggcgagacga cgacctaccg
      841 ggagttggcc cgccgcgctg ggtcgcccgc ggcctgccgg gcggtgggcg gtgtgatggc
      901 ggccaatcgg gcggtgctgc tgatgccgtg ccaccgggtg ctgccgacgg gggggcgggc
      961 cgaggcgccg gggcaattcg gcgcggggac ggcgatgaaa agccggctcc ttcggcacga
     1021 acggaggctg cgggcgattt ccgtttgagc ttggaggttc acgacggccg atgctgtccc
     1081 gtcagctcgc gcaattccgt caggattcac cattcattaa caattgccgg cggatcagcc
     1141 ccttcgtcaa ctgcggccgg tccatcccct tcttgaattc ctggcgtacg caccgggccg
     1201 gtgcgcttga aaaacctcat ggcaatctgt catggcccca aaggttaaac gctgcacaaa
     1261 tcgtcgcgtg ggtcggttgt cttccgttgg cgccagattc atggcgcggg gtgaggcctt
     1321 aatgagtccg tcgtcaagaa tttatgatcc aaaagacgcc gaattcatac aatttggcaa
     1381 attcgaaccg aatgcgccca ccgggatggc cggttgaaat gacttagaca ggcggattgc
     1441 ctgaccggtt tctgcatcaa aaaaaaactg ttttttccgg gttgtctttt caatttccgg
     1501 tgtgatagct tccctaacag ggtacggatg ctaactataa ccaccgcagg aatatcaagg
     1561 gaatcgaacc tgacctgctt gctgtctgtt gctggcaatc gctgcctcaa gccttccgtg
     1621 tcatcatcgc attgctggct catgctttcc ctaactggct tcgcggagaa tccgctgctc
     1681 gcgccaggtc gattcacctt caggagacac gaccgtgatg aagaactacc tcggtgagat
     1741 tatctatgcc agccggcaga aggataatct cacacaggat cagtacggcg cccgctacaa
     1801 tgtttcgggc cccgcaatct tcaagttcga aaaggggtat gtgaagccct cgctcgagct
     1861 gtggctgaag atggcggccg acgctgccat ctcggaacgc cgggcggtgc tgctctgggt
     1921 caagagcaaa ctgcccgaag agtatcagga gtatatcgag ctccagagtg ccgccgtcgt
     1981 cgagagcgag gcggaatcgg tcaagaagaa gggcaagaag cccgactact cgaagctgga
     2041 gaatcgcgag cagatgcgcg agttcctgga caaggacaag gcgatgccca aggggctgcg
     2101 cgacctgctc aaggacgacg agttgtgggc gctctacaag cccacgggac acgagatcaa
     2161 catgctgcgc gacatcttcg ggccgctcgg ccgcgggtcg aagaccgcct accgtgaagc
     2221 gctgcggctg atccgggaat tcgcgcactc cttctaatcg gcgccaggca ggaggccggc
     2281 gctcgccgcg aggagaggcc ccgcctgaac ggtgttccat cgatccgatc ccccgccggg
     2341 accatgcacc cggcggggga tctcgctttt gactcggcgg cgcgccggcg cgcggcgggt
     2401 ctggcgtttg cgcgccgggg cgcgctatga tttgaccgcg cgtcgacgcg atccagatcc
     2461 catcgcccga gacccgccgc ccgtgaaacc tggcttccca atcctgatgt accatgcgct
     2521 gtggccggcg gggctgtcgc ccgcggaggt cgacgcgcat tgccggcgcg acccgcaact
     2581 ggcggacccc ggggcgcggc gctatgcgct ggatgcggcg gtcttcgacc ggcagcttga
     2641 gcagcttgcc cgccatgccg cgccgatggc cgcgcagtgg gacgacctgg cgcgggggcg
     2701 gggcgcgccg cggcaggcgt ggatcacgtt cgatgacggc catgcgtcga atcacgagct
     2761 ggcgctgccg ctgctgcggc ggcgcgggct gcgcgcggcg ttcttcatca ccaccgactg
     2821 gatcggcgcc gagcggttca tgacgccggc gatgctgcgc gcgctgcgcg acgccggcat
     2881 gctgctgggc agccacggct gctcgcaccg ttacctgtcg gacctgacga ccgccgaact
     2941 ggaccgcgag ctgcgcgaat ccaaggcgcg gctggaggag ctgctgagcg aggaaattcc
     3001 ggcgctggcg ttgccgggcg ggcggggcga cgggcgggtt cgcgagcggg cgcgggcggc
     3061 cggctatcgt tatctgttca cgtcgcgggt cgggctggcg ccgccggagg gggatccgct
     3121 ggcgctgccg cgggtgccga tcgtcaatga tctacccgag gagtttctga gccggctgct
     3181 ggcgggggag acggccgagc tggcgcggat ggcgcgcggg gcgcggcggc gcgagttcgc
     3241 ccggcggatg ctgggcaacc ggatttacga ccggctgcga gggtgggtga tgagggaata
     3301 ggaattcaca attcgggtag agttttgggg gcgaggacga ggacgggaga cgatctttga
     3361 gtgagggatg atatgagaac gccccggctc gggatgagcc ggggcgttgg aatggcgcgc
     3421 gtaatcccgc ggttactcct cgctgtattg gcgttggagc atcgagacga cggcggggtc
     3481 ggccagggtg gtggtgtcgc cgagcatgcg gccctcggcg atgtctttga gcagacggcg
     3541 catgatcttg cccgagcggg tcttgggcag gtcggcggtg aggatgatct cgtcggggcg
     3601 ggcgatggcg ccgatcttgt tcgcgacgtg ttccttgagc ttttcgaaga cgttgtcgct
     3661 gacctcggcg ctttccttca tggtgacgaa ggcgacgagg gcctggccct tgacgtcgtg
     3721 ggcgcggccg acgcaggcgg cctcggcaac
//
LOCUS       DAOPCO010000358         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19934_length_3740_cov_4.008955, whole genome shotgun sequence.
ACCESSION   DAOPCO010000358 DAOPCO010000000
VERSION     DAOPCO010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19934_length_3740_cov_4.008955"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..927)
                     /locus_tag="PLS90_16270"
     CDS             complement(145..927)
                     /locus_tag="PLS90_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04003.1"
                     /translation="MAPTHNATQPRMSSVIPPFLGRWARAGAGRLPPALRDALMIFHV
                     QTQLIFSRTILLILACVFAFFTWLAIVAGIHQPNQAYDFLALQSIVMGLLTCMNLWSA
                     ESDGGTLELLMMRMRGPGHLIWFKLRVALVWMMILSFPLFAGLGWFVDLGLGRLLVFW
                     VFSMMAALPMMLLTCVVTTFVRNGLTASVLAVVMAVPVAALTENVPLRWIRFFRLFIP
                     PYKLLEESLPTILGNRLMWLAAVACLVWWLYNRLRRVERWIR"
     gene            complement(911..1828)
                     /locus_tag="PLS90_16275"
     CDS             complement(911..1828)
                     /locus_tag="PLS90_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK04004.1"
                     /translation="MSLRIEQLRKVYSDGKVALHGLDLELTPGVFGLLGPNGAGKSTL
                     LEILSLNLMPTSGRVLWDGRDIHRQPNAYRRILGYLPQSYGFYPELTVRAFLHYMGGL
                     YGLGGRRLRRRIAECLELVGLSAEQRKKTNALSGGMRQRLAIAHALLHEPKLLIVDEP
                     TTGLDPAERVAFRNLLFDLGRSATVILSTHIVKDVEFTCHRMMLLYDGRARFLGPPAD
                     FIERVRGRVLLTEIPAREFESFSRAHDVVAIQERGDRMKVRFLNGAPATNHGSPPGVR
                     PAAPNLEDAYVDFIRERRDEQEAPVGSDA"
     gene            complement(2065..3507)
                     /locus_tag="PLS90_16280"
     CDS             complement(2065..3507)
                     /locus_tag="PLS90_16280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPK04005.1"
                     /translation="MKSCRIASHCLVALGLLLAGPLPAAELFSDTIGYLNANAATDDG
                     DDSGLQIIPDRRGEWLAVWDTLTSLTGTSAGADIVVSRSSGSGSVWSPPTPISSIAAF
                     DDGGDEDPDAATDGHRNWVIVWESKATSGTQMAGDDADILSVRSTDGGLTWEPTGVLN
                     SNAWEDNDFDPKNEDLNPDVDTNRKEIWITVWQTEFNLGGTLGNDSDLLFARSIDKGA
                     TWSIARPLNTNAAFDAGNDNNPRLLYGGGSTWMCVWDSGDPLGGVDDGSQDIAYARSH
                     NDGLTWEAPRTLNSNAPIPSVDLDPSVATDERGNWVVVWESGATLGGTIGVDRDILFA
                     RSEDNGETWSEAAPLNTTAATDFTSTQVLHDRQPEIFTDTYGTWVCVWFTTDLQGVPE
                     SHQAINMAISTDNGATWTDPVTLDDSFYSQSDLDVDPIVRNDRLGHWIVGWRANNQPG
                     GGIGDTDLLFKPGLPQPIPSTSAKGWPLYD"
BASE COUNT          640 a         1099 c         1381 g          620 t
ORIGIN      
        1 gtagacgtaa tgcgtcgggg cggggcggcg ggtgctgtgc tcctcctgcc agaaaaaggg
       61 gaatagttga atataattga tgttatgcca gcgtcaagac aaatggactc tatgaataca
      121 taaagctggt gggggccggg ccggtcagcg gatccagcgc tccacgcggc ggaggcgatt
      181 gtagagccac cagacgaggc aggcgacggc ggcgagccac atcaggcggt tgccgaggat
      241 ggtcggcagg gattcctcga gcagcttgta gggcgggatg aagagccgga aaaaacggat
      301 ccagcgcagc ggcacgtttt cggtcagcgc ggcgacgggg accgccatga cgacggccag
      361 cacgctcgcg gtcaggccgt tgcggacgaa cgtcgtcacg acgcaggtca gcagcatcat
      421 cggcagggcg gccatcatgc tgaagaccca gaagacgagc agccggccga ggcccagatc
      481 gacaaaccag ccgaggcccg caaacagcgg gaaggagagg atcatcatcc aaaccagcgc
      541 gacgcgcagc ttgaaccaga tcagatgccc cggcccgcgc atccgcatca tcagcagctc
      601 gagggtgccg ccgtcgcttt cggcgctcca caggttcatg caggtcagca ggcccatgac
      661 gatcgactga agcgcgagga aatcgtaagc ctggttgggc tgatgaatcc cggcgacgat
      721 ggcgagccag gtgaagaaag cgaagacgca ggccaggatg agcaggatcg tccgggagaa
      781 gatgagctgc gtctggacgt ggaagatcat cagcgcatcg cgcagggcgg ggggcaggcg
      841 gccggcgccg gcgcgggccc agcgcccgag gaagggcggg atgacgctcg acatgcgcgg
      901 ctgcgttgcg ttatgcgtcg gagccaacgg gcgcctcctg ctcgtcgcgg cgttcgcgaa
      961 taaaatcgac gtaggcatcc tccagattgg gcgccgcggg gcggacgccc ggcggagagc
     1021 catggttggt cgccggggcg ccgttcagga agcgcacttt catgcggtcg ccccgttcct
     1081 ggatcgcgac cacatcgtgg gcgcggctga acgattcgaa ctcgcgggcg gggatctcgg
     1141 tcagcagcac gcggccgcgg acgcgctcga tgaagtcggc cgggggcccg aggaagcgcg
     1201 cccgcccgtc atagagcagc atcatccggt ggcaggtgaa ctcgacgtcc ttgacgatgt
     1261 gcgtcgagag gatcacggtg gccgagcggc ccaggtcgaa cagcaggttg cgaaaggcga
     1321 cgcgctcggc cggatcgagc ccggtcgtcg gctcatccac gatcagcagc ttgggctcgt
     1381 gcagcagggc gtgcgcgatc gcgaggcgct gacgcatgcc gccggagagg gcgttcgtct
     1441 tcttgcgctg ctcggcggac aggccgacca gttccaggca ctcggcgatg cgccggcgca
     1501 gccgccgtcc gcccagtccg tacaggccgc ccatgtaatg gaggaaggcg cgcacggtca
     1561 gctcggggta gaagccgtag ctttgcggca gatagcccag gatgcggcga taggcgttcg
     1621 gctggcggtg gatgtcgcgg ccgtcccaca gcacgcggcc ggaggtcggc atcaggttga
     1681 gcgagaggat ttccagcagc gtggacttgc cggcgccgtt gggtccgagc aggccgaaga
     1741 ccccgggcgt cagttccagg tccaggccgt gcagggcgac cttgccgtcg ctgtaaacct
     1801 tgcggagctg ttcgatccga agcgacatga aaggagatcg agctcccgtc gcgcggcgtt
     1861 gggcgcatcc gcggccgcat gctggaacca tcccgccgcg cgcggcgggt cgtctctatc
     1921 tccctagtgt tgcaggaccg acgaaagttg ccgagagaaa tgcggatcgt cgtagaaggc
     1981 gggccgcccc gcgagtggcg gggcggcgcc ggccgacggt tttgagttga acacgctggc
     2041 gcggggcacg ccgtccggtg gcgctcaatc gtagagcggc cagcctttgg ccgatgtcga
     2101 ggggatcggc tgcgggaggc cgggcttgaa gagcagatcc gtgtcgccga tgccgccgcc
     2161 gggctgattg ttcgcgcgcc agccgacgat ccagtgtccc aggcggtcgt tgcggacgat
     2221 cgggtcgacg tccagatccg actggctgta gaagctgtcg tccagcgtca cgggatcggt
     2281 ccaggtcgcg ccgttgtcgg tcgaaatcgc catattgatt gcctgatgcg attcgggcac
     2341 gccctgcagg tcggtcgtga accagacgca gacccaggtg ccataggtgt cggtgaagat
     2401 ctccggttgg cgatcgtgga ggacctgggt ggaggtgaaa tcggtggcgg cggtggtatt
     2461 gaggggcgcc gcctcgctcc aggtttcgcc gttatcctcg gagcgggcga agaggatgtc
     2521 gcggtcgaca ccgatggtgc cgccgagcgt cgcgccggat tcccagacga cgacccaatt
     2581 gccgcgctcg tcggtcgcga cgctggggtc gaggtcgacg ctcggaatcg gcgcattgga
     2641 attgagcgtg cgcggggcct cccacgtcag gccgtcgttg tgcgagcggg cgtaggcgat
     2701 gtcctgtgag ccgtcgtcga cgccgcccaa ggggtcgccg gagtcccaga cgcacatcca
     2761 cgtgctgccg ccgccgtaaa gcaggcgggg gttgttgtcg ttgcccgcgt cgaaggcggc
     2821 gttggtgttc agcggccggg cgatgctcca agtagcgccc ttgtcgatcg agcgcgcaaa
     2881 gagcaggtcc gaatcgttcc ccagggttcc gcccaggttg aattcggtct gccagaccgt
     2941 gatccagatt tccttgcggt tggtgtcgac gtcggggttg aggtcttcgt tcttggggtc
     3001 gaagtcgttg tcttcccagg cattgctgtt cagcacgccg gtcggttccc aagttagccc
     3061 gccgtcagtg gagcgcacgg agaggatatc ggcgtcatcg ccggccatct gcgtgccgct
     3121 ggtcgctttg gattcccaga cgatgaccca gttgcggtgg ccgtcggtgg cggcgtcggg
     3181 gtcctcgtcg ccgccgtcgt cgaaggcggc aatcgagctg atcggcgtcg ggggggacca
     3241 gacgctgccg gagccggagg agcgggaaac gacgatgtcg gctccggccg aggttccggt
     3301 cagagaggtt aacgtatccc acacggccag ccattcgccg cggcggtcgg ggatgatctg
     3361 cagcccggag tcatcgccgt cgtcggtggc ggcgttggcg ttcaggtatc cgatggtatc
     3421 ggagaacagc tcggcggcgg ggagaggccc cgcgagcagc aaacccagcg ccaccaggca
     3481 gtggctcgca atccggcatg acttcatcga cggcaccttc ctggttctga tgcaatgacc
     3541 cggagtccca aagtcaatgg ggttgggcac agtacggacg tgtcgctttt tccttatcgg
     3601 cgggtcgggg cggcacatta gtttgtcgcg gggcgggttc cgccgtgttg gattaagggc
     3661 gtcgggtcaa ttcaccaccg gtggcatccc tccgggatgc gggagacggg ggggtgccgg
     3721 gtgaccggtg gtatcgctgc
//
LOCUS       DAOPCO010000359         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_19991_length_3731_cov_4.693145, whole genome shotgun sequence.
ACCESSION   DAOPCO010000359 DAOPCO010000000
VERSION     DAOPCO010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19991_length_3731_cov_4.693145"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..196)
                     /locus_tag="PLS90_16285"
     tRNA            complement(120..196)
                     /locus_tag="PLS90_16285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Met,seq:cat)
     gene            complement(312..387)
                     /locus_tag="PLS90_16290"
     tRNA            complement(312..387)
                     /locus_tag="PLS90_16290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(352..354),aa:Met,seq:cat)
     gene            727..1680
                     /gene="grpE"
                     /locus_tag="PLS90_16295"
     CDS             727..1680
                     /gene="grpE"
                     /locus_tag="PLS90_16295"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPK04006.1"
                     /translation="MPLPTPNPVRRLSRLKDYFFPQEFVVDERPLPVYSPRPERIERP
                     RRRRKKRSSAPPPAPPAEVEVDQAPALEEAVPADSADAAQPEALPEAPTDAIEAVFGD
                     ETDQPPAGEPLEDAAEPAEDPGVEQVVPAAAAAESASPAQAEAAQPLADSDVEEEVEE
                     EQPEGQHQPPSQRDLLTQMTELLAEQARLELKTRQLEMNAGAGDDEFGRFVRQLLPFV
                     DNYSHLLDMAREQSHDPELDAWLRNIEALYFRIIRLLEDFGLRFISSVGKQVDLDYQE
                     VVEYRRTDEYPHNTVIRELQKGAVFRGKLLRDAKVVVACNE"
     gene            1842..3596
                     /gene="dnaK"
                     /locus_tag="PLS90_16300"
     CDS             1842..3596
                     /gene="dnaK"
                     /locus_tag="PLS90_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPK04007.1"
                     /translation="MPRIVGIDLGTTNSVVAVMDRNGPVVIPNSSGERLTASVGFTKS
                     GEILVGKKARRAAVMNVGRTVFSIKRHMGTTYRVRIDGREYTPQEISGMILQKLKQDT
                     EDFFGEEVSQAVITVPAYFTDAQRQATKDAGEIAGFNVRRVIDEPTAAAMAYGLDKSK
                     DQTIIVYDLGGGTFDVSIIEVMSGVFRVMAIHGDTQLGGDDFDKRIMDFLVEEFKKEH
                     GVNLEDDPVAMMRLRDAAEEAKIDLSEMTETHILVEAITMGDKGPLTLDVQLKRTQFE
                     SMIRDLVERTIEPMQSAIKDAGLSRDQIDTVLLVGGSTRIPLVQNIVKQTLGKNPNRD
                     ISPDEVVALGAAVQTLVLAPVEGDLEDSLATHYGKEKPVIIHMTPFSLGVALQSDQFA
                     PIIERNSTYPTEAKDIFTTTRDFQEAISFPIYEGEEPIASENTFLDLLRIEGITPAPR
                     GVPRIEVTFRLNADRILEARAEDLATGLEKTITIASTGSRLSDAEKNRMVREARERIA
                     QTLRERIKSNVREEATDAIRRGQELIAHDPGNPRAQQVRQLISTVERGIQSGDDDNLE
                     RYTTELIQLVNELEAASA"
BASE COUNT          733 a         1273 c         1067 g          658 t
ORIGIN      
        1 cccttcgtgc agaaaggaca cctgagcggc gttttttgtt tgaagtcggg cgacacggcc
       61 ggaattgccc gccagagcct gcggcgcggc cgtcaaaaaa gataagagtg aatgaaggct
      121 ggtggcgggg ggcggaatcg aaccgccgac ctacgggtta tgaatccgtc gctctaaccg
      181 cctgagctac cccgccgtac gctggaaccg acgcgccggg ttgccttggg caagcatcca
      241 tccgacgctg aatccattgg ctttagataa aacggaccag ccatcgccga cggctggtcc
      301 gttcatcaaa ctggtagcgg ggggcggact cgaaccgccg acctttgggt tatgagccca
      361 acgagctacc aactgctcca ccccgcatca cgttccggtc cgaccggaca cagggcctta
      421 cgatagcgga tcgagccgaa ccttgtcaat gccccgaagc cgactttttt gcgccgaacg
      481 acacccccca ggatcgctct ccctgatcgg tattcggtaa cggcatcggt cttttttcat
      541 ttatatcttt ttttaccttt acgcccctta tgtctcttcc cactttcgtc ccacccctct
      601 tcactaccca tcgatttttg tctttcatag tccttgtcaa tttctgcctg ctgacctatg
      661 atataaggtg agttgcgcgt tcgccactca accgcgctga aacaacctga cttccgcgaa
      721 ttggaaatgc ccctgccgac tcccaatccc gtccgccgcc tctcgcggct caaggattac
      781 ttcttccccc aggaattcgt cgtcgacgaa cggccgttgc cggtctacag cccccggccg
      841 gagcggatcg aacgtccgcg ccggcggcgg aagaagcggt cctccgcgcc cccgcccgcg
      901 ccccccgccg aggtggaagt ggatcaagct cccgccctcg aggaagccgt ccccgccgat
      961 tccgccgacg ccgcccagcc ggaagccctt ccggaagccc ccaccgacgc gatcgaggcc
     1021 gtcttcggcg acgaaaccga tcagcctccc gcgggcgagc ccctcgagga cgccgccgag
     1081 ccggccgagg atcccggcgt cgagcaggtc gttccggccg ccgcggcggc cgaatctgcg
     1141 tccccggcgc aggcggaggc cgcccaaccc cttgccgatt ccgacgtcga ggaggaggtg
     1201 gaggaagagc agccggaagg ccagcaccag cctccgtccc agcgtgatct gctgacgcaa
     1261 atgaccgaac tgctggccga gcaggcgcgg ctggagttga aaaccaggca actggaaatg
     1321 aacgccggcg ccggcgacga cgaattcggc cgtttcgtcc gtcaactgct ccccttcgtc
     1381 gataattact cccacctgct ggacatggcc cgcgaacaat cgcatgaccc cgagctcgac
     1441 gcctggctgc gcaacatcga agcgctctat ttccgcatca tccggctgct ggaggatttc
     1501 ggcctgcggt tcatcagttc agtcggcaaa caggtcgatc tggactacca ggaggttgtc
     1561 gagtatcgcc gcaccgacga gtatccccac aacaccgtca ttcgcgagtt gcagaaggga
     1621 gccgtcttcc gcggcaagct gctgcgcgac gccaaggtcg tcgtcgcctg caatgaataa
     1681 tcttgccgcc cggccgacgt tttctgtcgt cagttcgctg aattgagagt tactatatgc
     1741 cccggtcttg taccgcggat ttcatttcat atcagttcct tgcgtggccc ggcgcggcca
     1801 catccgcatt ccatcccttt ccacctgtcg gagtatgaat catgccgcgt atcgttggaa
     1861 ttgacctggg aacgaccaac tccgtcgtgg ccgtcatgga tcggaacggc ccggtcgtca
     1921 ttcccaacag ctcgggggaa cgcctgacgg cgtcggtcgg cttcaccaag agcggcgaaa
     1981 tcctggtcgg taagaaggcg cgccgcgccg ccgtgatgaa cgtcgggcgc accgtcttct
     2041 ccatcaagcg ccatatgggc accacctatc gggtccgcat cgatggccgc gaatacaccc
     2101 cccaggaaat ctcgggcatg attctgcaaa agctcaagca ggacaccgag gatttcttcg
     2161 gcgaggaggt cagccaggcg gtcatcaccg tcccggccta ctttaccgac gcccagcgcc
     2221 aggccaccaa ggacgccggc gagatcgccg gtttcaacgt ccgccgcgtg atcgacgagc
     2281 ccaccgccgc cgccatggcc tatggcctcg acaagagcaa ggaccagacg attatcgtgt
     2341 acgatctcgg cggcggcacg ttcgacgtct cgatcatcga ggtcatgtcg ggcgtgttcc
     2401 gagtcatggc gatccacggc gatacgcagc tcggcggcga tgatttcgac aagcgcatca
     2461 tggatttcct cgtcgaggaa ttcaagaaag agcatggcgt caacctggag gacgatcccg
     2521 tcgcgatgat gcgcctgcgc gacgccgccg aggaagccaa gatcgatctg tccgaaatga
     2581 ccgagacgca catcctcgtc gaagccatca cgatgggcga caagggaccg ctgacgctcg
     2641 atgtccagct caagcgcacg cagttcgagt cgatgatccg cgacctggtc gagcggacga
     2701 tcgagccgat gcagtcggcg atcaaggacg ccggcctctc gcgcgaccag atcgacacgg
     2761 tcctgctcgt cggcggctcc acgcgcatcc cgctcgttca gaacatcgtc aagcagacgc
     2821 tgggcaagaa tcccaaccgc gacatctccc ccgacgaagt cgtcgccctc ggcgcggccg
     2881 tccagaccct cgtcctggcg ccggtcgaag gcgacctcga ggattccctc gccacgcatt
     2941 acggcaagga aaagccggtc atcatccaca tgaccccgtt cagtctcggc gtcgcgctcc
     3001 agagcgacca gtttgcgccg atcatcgaac gcaattccac ctacccgacc gaggccaagg
     3061 acatcttcac cacgacccgc gacttccagg aggcgatctc gttcccgatt tacgaggggg
     3121 aagagccgat cgcgtcggag aacaccttcc tcgatctgct gcggatcgag gggattaccc
     3181 cggcgccgcg cggcgtcccg cgcatcgagg tcaccttccg cctcaacgcc gaccggatcc
     3241 tcgaggcccg ggccgaggac ctcgccaccg gcctcgaaaa aacgatcacg atcgcctcga
     3301 ccggatcgcg cctctccgat gcggagaaga accgcatggt gcgcgaggcg cgtgaacgga
     3361 tcgcccagac gctgcgcgaa cggatcaagt ccaacgtccg cgaggaggcg accgacgcca
     3421 tccgccgcgg ccaggaactg atcgcgcatg atccgggcaa cccccgcgcc cagcaggtgc
     3481 gccaattgat cagcaccgtc gagcgcggca tccagtcggg tgacgacgac aacctcgaac
     3541 gctacacgac cgagctgatc caactggtca atgaactgga agcggcatcg gcctgacccc
     3601 ccccacccgc gactgatgcg tagtaatcgg tatcgagatc ggtatcgcta tcggagtgga
     3661 ttatcggtac cgctatcttc ggtcgggatc gattcatccc cccccgcatc ccggagggat
     3721 gcccgatcgt a
//
LOCUS       DAOPCO010000360         3677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20364_length_3677_cov_5.901436, whole genome shotgun sequence.
ACCESSION   DAOPCO010000360 DAOPCO010000000
VERSION     DAOPCO010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20364_length_3677_cov_5.901436"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..1186
                     /gene="hemB"
                     /locus_tag="PLS90_16305"
     CDS             197..1186
                     /gene="hemB"
                     /locus_tag="PLS90_16305"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPK04008.1"
                     /translation="MPAAFPIRRLRRLRMNPAIRSMVRETRLAREDLVQPLFVCSGQG
                     VRREVPSMPGVFNLSVDLLVEECREVADLGIPAVLLFGIPESKDDQATGAWHDQGIVQ
                     QGLRAVRAALGDRLVLIADTCLCEYTSHGHCGIVGERGEIQNDPTLAVLARTAVSQAR
                     AGADVIAPSDMMDGRVAAIRRALDDAGLSNTPILSYAVKYASACYGPFRDAAQSPPSF
                     GDRKSHQMDPANAREALEEARSDLEEGADLLMVKPAGPNLDILAALRRDVNVPLAAYQ
                     VSGEYAMVKAAGARGWIDEAAVTWELALAIKRAGADLIITYFAKDLARRLGRS"
     gene            1273..1935
                     /locus_tag="PLS90_16310"
     CDS             1273..1935
                     /locus_tag="PLS90_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04009.1"
                     /translation="MRFLPLLIMLLAGAAGAIGIALTPGAGAAAAGMFLLTGLLIGGG
                     GRLAGARGAAAVAGPALFLLACAAGIGFSWPRAMRALAVADVGDALTEGAYREFAEDS
                     EILMNIEESQYPEFMVKRNYTDAARPEDVTEKELQQFWIGTVPTLIEMQRTHPSYETW
                     RERQLQVVADSHKLRQPLPKFAAARLSGLALLGALAGLVLSALPALRKPAPPAPETSE
                     TP"
     gene            complement(1954..>3677)
                     /locus_tag="PLS90_16315"
     CDS             complement(1954..>3677)
                     /locus_tag="PLS90_16315"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04010.1"
                     /translation="VRQPELWWPNGMGDQPLYDVAVELLDAGGSVLDTQRKRIGLRTL
                     NVRRRKDRWGESFEFVVNRVPFFAKGADWIPADVFAPRLTRDDYARLLGDAAAVHMNM
                     LRVWGGGIYEDDAFYAICDELGLCVWQDMMFSCATYPTFDEGWMANVEAEARDNIRRL
                     RHHPAIALWCGNNEVEFSVFVGDQWTEDRMSWESYGRLFDELLPRVAKELAPDIDYWP
                     GSPHTPVGDRAKTEDPHSGDAHLWDVWHGRQPFEWYHGAFHRFVSEFGFQSFPEPKTV
                     RGYTLPADRNLVSRIMEHHQRSYIGNTAIMQYLLDWFRLPGDFDSQLWLSQILQGLGI
                     RFGVEHWRRNMPRCMGALYWQLNDCWPVASWASIDYHGRWKALHYMARRFFAPLLVSG
                     VPDVQRRKVDLHVASDRGEAVDGRLRWRVTTVGGRTLEEAERAIRIAPRKSTPCGALA
                     LAGPLAQHPARDLIVWLALDVDGAVVSENTVLLARPKHMDLERPAFQTRVRPLKDGTF
                     SVRVKSDKPALWVWLELRRDDARFSDNFFDLPAGGAREIVIRPARKLSREALERQLSI
                     RSLVDTY"
BASE COUNT          581 a         1220 c         1298 g          578 t
ORIGIN      
        1 gcgctctaca gcgacatggc gccgcggctg gtcgtcggcg ggacggacga accggcgcgg
       61 taggcgtggt gggcgcggct tcttccccgg ccggcgccgt tggccgcggg cggcgaaggc
      121 ggcgcgccgc cagcatggaa aatcgcgcgc cgcccgctag aatgacgtga accctttagc
      181 cgggagccgc ctgccgatgc ctgccgcttt tccgatacgc cgcctcagac gcctgaggat
      241 gaaccccgcg atccgctcga tggtgcgcga gacgcgcctg gcgcgggagg acctggtcca
      301 gccgctgttc gtctgctccg gccagggggt gcgccgcgaa gtgccctcga tgcccggggt
      361 gttcaatctt tcggtggacc tgttggtgga ggaatgccgc gaagtggccg acctggggat
      421 cccggccgtc ctgctgttcg gcattccgga gagcaaggat gaccaggcga cgggcgcgtg
      481 gcatgaccag gggatcgtgc agcaggggct gcgagcggtg cgggcggcgc tgggcgaccg
      541 gctggtcctg atcgccgata cctgcctgtg cgaatatacc agccacggcc actgcgggat
      601 cgtcggcgag cggggcgaga tccagaatga cccgacgctg gccgtgctgg cccggacggc
      661 cgtttcgcag gcgcgggccg gggccgatgt gatcgcgccg tcggacatga tggacggccg
      721 cgtggcggcg atccgccgcg cgctggacga cgcggggctg agcaacacgc cgatcctgtc
      781 ctatgcggtc aaatacgcct cggcgtgtta cgggccgttc cgcgacgcgg cgcagagtcc
      841 gcccagcttc ggcgatcgca agagccacca gatggacccg gccaacgcgc gcgaggcgct
      901 ggaggaggcg cgcagcgacc tggaggaggg ggccgacctg ctgatggtca agccggccgg
      961 cccgaacctg gacattctgg cggccctccg gcgcgacgtg aacgtgccgc tggcggcgta
     1021 ccaggtctcg ggcgaatatg cgatggtgaa ggcggccggc gcgcgcggct ggatcgatga
     1081 ggccgccgtg acatgggaac tggcgctggc gatcaagcgc gccggggcgg acctgatcat
     1141 tacctatttc gcgaaagacc tggcgcggag gctggggcgg agctgaccgg ccccatgctt
     1201 cggcggcgga ggttggcatt ggaccgacac tccgttgcgt ggtcacgatt ccccgtcaga
     1261 aagataacct taatgagatt tctgccactc ctgatcatgc tgctggcggg ggccgccggc
     1321 gcaatcggca ttgcgctgac ccccggcgcc ggcgcggcgg cggcgggcat gtttctcctg
     1381 accggcctgc tgatcggcgg cgggggccgg ctggcgggcg cgcgcggcgc ggcggcggtg
     1441 gccggaccgg cgctgtttct gctcgcctgc gccgccggca tcggcttttc ctggccgcgc
     1501 gcgatgcggg cgctggccgt ggccgatgtc ggcgatgcgc tgaccgaagg ggcctatcgc
     1561 gagttcgccg aagattccga aattttaatg aatattgagg aaagtcagta tcccgagttc
     1621 atggtgaagc gcaattacac cgacgccgcg cggcccgagg acgtgacgga gaaggaactg
     1681 caacaattct ggatcggcac cgtgccgacg ctgatcgaga tgcaacggac gcatccgtcc
     1741 tacgagacgt ggcgcgaacg gcaactgcag gtcgtagccg attcccacaa gctgcggcag
     1801 ccgctgccga aattcgcggc cgcgcgcctg tcgggcctgg cgctgctcgg cgcgctggcg
     1861 ggtctcgtcc tgtcggcgtt gccggcgctg cgcaaaccgg cgccccccgc gccggaaacc
     1921 tcggaaaccc cctgaactat cgcgtcgatc gaatcaatag gtatcgacca ggctgcgaat
     1981 gcttaactgc cgctcgaggg cctcgcgcga gagcttgcgc gcggggcgga tcacgatttc
     2041 cctcgccccg cccgccggca ggtcgaagaa attatccgag aaccgcgcat cgtcccggcg
     2101 caattccagc cagacccaca acgccggctt gtcgctcttg acgcgcacgc tgaatgtccc
     2161 atccttcagc ggccgcacgc gcgtctgaaa tgccggccgc tccaggtcca tgtgtttcgg
     2221 ccgggcgagc agcacggtat tttctgagac gaccgcgccg tcgacatcga gcgcgagcca
     2281 cacgatcagg tcgcgggcgg ggtgttgcgc gagcggcccg gccagcgcca gcgcgccgca
     2341 gggggtcgat ttgcggggcg cgatccgaat cgcccgctcg gcctcctcca gcgtcctgcc
     2401 gccgacggtc gtgacgcgcc agcgcagccg gccgtcgacg gcctcgcccc ggtcgctcgc
     2461 cacgtgcaga tccaccttgc gccgctgcac gtcgggcacg ccgctcacca gcagcggggc
     2521 gaagaatcgc cgggccatgt agtgcagcgc cttccagcgg ccgtgataat cgatcgaggc
     2581 ccagctcgcc acgggccagc aatcgttgag ctgccagtaa agcgccccca tgcagcgcgg
     2641 catgttgcgc cgccagtgct cgacgccgaa ccgaatgccc agcccctgga ggatctgcga
     2701 gagccagagc tggctgtcga aatcgcccgg cagccgaaac caatccagca ggtactgcat
     2761 gatcgccgtg ttgccgatgt agctgcgctg gtggtgttcc atgatgcggc tgaccagatt
     2821 gcggtccgcg ggcagcgtgt agccgcgcac tgttttcggt tcggggaacg actggaaacc
     2881 gaactcgctg acgaaccggt gaaaggcgcc gtgataccac tcaaacggct gacggccgtg
     2941 ccacacatcc cacagatggg cgtcgccgct atgcgggtct tcggtcttcg cgcggtcgcc
     3001 gacgggggtg tgcgggctcc ccggccagta gtcgatgtcg ggggccagtt ccttcgcgac
     3061 gcgcggcagc agctcgtcga acagccgccc gtagctctcc cagctcatcc ggtcctcggt
     3121 ccactggtcg ccgacgaaga cgctgaattc gacttcattg ttgccgcacc agagggcgat
     3181 ggcgggatga tggcgcaggc ggcggatatt gtcgcgcgcc tccgcctcga cgttcgccat
     3241 ccagccctcg tcaaatgtcg gataggtggc gcaggagaac atcatatcct gccagacgca
     3301 caggcccagc tcatcgcaga tcgcgtagaa ggcgtcgtcc tcgtagatgc cgccgcccca
     3361 gacgcgcagc atgttcatat ggacggcggc ggcgtcgccg agcaggcggg cgtaatcgtc
     3421 gcgcgtgagg cgcggcgcga agacgtcggc cggtatccag tcggccccct tggcgaagaa
     3481 cggcacgcgg ttgacgacga actcgaacga ctcgccccag cggtccttcc gccgccgcac
     3541 gttgagcgtg cgcagtccga tccgcttgcg ctgcgtgtcg aggacggatc cgccggcgtc
     3601 gagcaattca acggcgacgt cgtacagggg ctggtcgccc atgccgttgg gccaccacag
     3661 ttcgggctgg cggacga
//
LOCUS       DAOPCO010000361         3676 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20374_length_3676_cov_4.958851, whole genome shotgun sequence.
ACCESSION   DAOPCO010000361 DAOPCO010000000
VERSION     DAOPCO010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20374_length_3676_cov_4.958851"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2949)
                     /locus_tag="PLS90_16320"
     CDS             complement(<1..2949)
                     /locus_tag="PLS90_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPK04011.1"
                     /translation="MVGLLCLTVALTSCRREPEQSPPVAEQTPPLRPWPDIDPHQTRP
                     ERPPAWQPAGSAAHLTAVESETTTLTLALRLPWDAPVPTRRTGFTVVLPAGATATPIV
                     DRVELGDRVVFDRGRTEDVPTTRAQSGFQPSAVRVESRGYLRQWPIHRLEIEPDFWAS
                     LRHAERAEGDDLSAILKIGWNDLPATATQDLPAADEANHGIARVAAHLVINPRDLARW
                     ATPSPPIPRAAEPFQPESLAPDAPGWMRVVVTETGLHRLSVDGLTTCGLIGNGFGLED
                     VRIFDGARPVPTHVGQQTTANGKSPLDGVYFYGWASQGPYSSRHVYWATVSKQAAAPE
                     WSTPPAFLLEEERQPLATIRGKERRDRDLTLQMLQDNFLNIRQMDWVEAELTNDQPFQ
                     FRMTPHAPVAPPAPQEARLHAHLYLHGPPEAWRGTELILSHGGRPIAKHRINGAGDTE
                     IDVAITTNSLLEAARPITLAVATPSSATLNADDELGIWLDWAELHYPAQPVLRDGRLR
                     LDGDAVTTAGLYRLSPARVFADAIDQAMAFALNPTAQAAAPLDWGEPAADSLPAIVRE
                     NWISEYWDMAAAIVPPVAPVAWRPDDLAAGRDADYLIITTPELEAALKPLIDLNAARG
                     LKSHLLHVDSLYDFFSDGALSPEAIRRYLAWLIEQAGTGAPDYVLLVGDSTSDYLNRT
                     RSPVVNQVPTYSFDYGSETWASDYWYTCVAGDDDLGDYLIGRLSLNNPDDLGRAVAKL
                     IEYAHGPQLGPWRATLAYVADNLEDFREAAEQARVDGTPAALDSRRIYLDRMPFEDNW
                     YVPKSYIEWVMAVERNWMKVSAHATDQIKAAFNEGVALLDFYGHGAPNLWTDERIFFG
                     GDSENRDTQYLAADGVYPFVTNFTCNTGAFDYPMTPWNICLSEDLLRTPHGGAIGLFV
                     PSGPGTTVVHRELAAQLRRALFQGWRARCRRRRSSVRRIRWRSSACSPASSCTSSLSA
                     AGALRAKMAAG"
     gene            3384..>3676
                     /locus_tag="PLS90_16325"
     CDS             3384..>3676
                     /locus_tag="PLS90_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04012.1"
                     /translation="MPPAARVNDPVSHPLPPVLNPGPGSPDVKIGFLPAWRGVPSAAA
                     ASIQSAKAISDAAIKSAEAATAAAAGTPGLPAAKTAEETTKANAATSMGSTITS"
BASE COUNT          603 a         1214 c         1245 g          614 t
ORIGIN      
        1 gcccgccgcc atcttcgcgc gcagggcacc agctgcggat aaagagctcg tgcaagagct
       61 ggcaggtgag cacgccgacg aacgccatct gatccgtctc actgaggagc gccgccgcct
      121 gcaccttgcg cgccagccct gaaacagcgc ccggcgcaat tgtgcggcca gctcgcggtg
      181 gacaacggtg gtgcccggac cggaggggac gaacaggccg atcgcgcccc cgtgcggcgt
      241 gcgcagcagg tcttcgctca ggcagatatt ccacggcgtc atcggataat cgaacgcgcc
      301 agtattgcag gtgaagttgg tgacgaacgg atagacgccg tcggccgcga ggtattgcgt
      361 gtcgcggttt tcggagtcgc cgccgaagaa aatgcgctcg tcggtccaga gattgggcgc
      421 cccgtggccg taaaagtcga gcagggcgac gccctcgttg aacgccgcct tgatctggtc
      481 ggtggcgtgc gcgctgacct tcatccagtt gcgttcgacc gccatcaccc attcgatata
      541 cgacttgggc acgtaccagt tgtcctcgaa cggcatccgg tccaggtaga tccggcgcga
      601 atccagcgcg gcgggtgtgc catcgacgcg ggcctgctcg gcggcttcgc gaaaatcctc
      661 caggttgtcc gcgacatagg ccagcgtcgc ccgccacgga cccagctgcg ggccatgcgc
      721 atactcgatc aacttggcga cggcacggcc cagatcgtcg gggttgttca gcgaaagccg
      781 accgatcagg tagtcgccca ggtcatcatc gccggcgacg caggtatacc agtagtccga
      841 ggcccaggtt tccgatccgt aatcgaacga ataggtcggc acctgattga ccaccggact
      901 gcgtgtccgg ttcaggtaat cgctcgtgct gtcgccgacc agcaggacgt agtccggggc
      961 gccggtcccg gcctgctcga tcagccaggc gaggtagcgc cggatggctt cgggcgagag
     1021 cgctccgtca ctgaagaaat cgtagagcga atcgacgtgg agcagatggg acttgagacc
     1081 gcgggcggcg ttcagatcga tgagtggctt gagtgcggct tccagttccg gcgtggtgat
     1141 gatcagatag tcggcgtcgc gtcccgccgc caggtcgtct gggcgccacg cgaccggagc
     1201 caccggagga acgatcgccg ccgccatgtc ccagtattcg ctgatccagt tttcgcgcac
     1261 gatcgcgggc agcgaatcgg cggcgggctc accccagtcg agcggggcgg ccgcctgggc
     1321 ggtgggattg agggcgaacg ccatcgcctg gtcgatcgcg tccgcaaaga cccgtgccgg
     1381 actgaggcga tacagccccg ccgtggtgac ggcgtcgccg tccagacgca ggcggccgtc
     1441 gcgcaacacc ggctgcgccg gatagtgcaa ctcggcccaa tccagccaga tgccgagttc
     1501 gtcgtcggcg ttgagcgtgg cgctcgaggg cgtggcgacg gcgagcgtga tcggccgcgc
     1561 cgcctcgagc agcgagttag tcgtgatcgc cacgtcgatt tcggtatcgc cggcgccgtt
     1621 gatccgatgc tttgcgatcg gccgaccccc atgcgacagg atcagctccg tcccccgcca
     1681 tgcttcgggc ggcccgtgca gatacaggtg ggcgtgcagg cgcgcctcct gtggggctgg
     1741 cggcgcgacg ggcgcgtgcg gcgtcatccg aaactgaaac ggctgatcat tggtcagctc
     1801 ggcctccacc cagtccatct ggcggatatt gaggaaatta tcctgaagca tctggagcgt
     1861 caggtcgcgg tcacggcgct ctttgccgcg aatcgtcgcc aacggctgtc gctcctcctc
     1921 aaggaggaac gccggcgggg tgctccattc cggcgcggcg gcctgttttg aaaccgtcgc
     1981 ccaatagacg tgccgcgagc tgtaaggacc ctgactggcc catccgtaga agtagacgcc
     2041 gtccaggggt gatttcccgt ttgcggttgt ttgctggccc acgtgggtcg ggacgggcct
     2101 ggcgccatcg aagatccgca cgtcttccag cccgaatccg ttgccgatca ggccgcaggt
     2161 ggtcaggccg tcaaccgaca accggtgaag ccccgtttcg gtgacaacga cccgcatcca
     2221 gccgggtgcg tcgggcgcaa gcgattccgg ttggaacggt tcggccgccc gcgggatcgg
     2281 cggtgacggc gtggcccacc gcgccaggtc gcgcgggttg atcaccagat gagcggccac
     2341 gcgcgcgatc ccgtggttgg cttcgtcggc ggcggggagg tcttgggtcg ccgtggcggg
     2401 gagatcattc cagccgattt tcaggatcgc cgatagatcg tccccttcgg cgcgctcggc
     2461 gtggcgcaac gacgcccaga aatccggctc aatttccagg cgatggatcg gccactggcg
     2521 cagatagccc cgcgattcga cccgaacagc cgaaggctga aatcccgact gcgcgcgcgt
     2581 ggtcggcaca tcctcggtcc ggcctctgtc gaaaacgacc cggtcgccca gttcaacgcg
     2641 gtcgacgatg ggcgtcgcgg tcgcccccgc gggcagaacc acggtaaacc cggtccggcg
     2701 cgtcggcacg ggggcatccc acggcagccg caacgccagc gtcaacgtcg tcgtttcgga
     2761 ttccaccgcc gtcaggtggg ctgcgctgcc ggccggctgc caggctggtg gtcgctcggg
     2821 tcgggtctga tggggatcaa tgtcgggcca cgggcgcagc ggcggggtct gttcggccac
     2881 gggcgggctc tgttcgggct cgcgccggca gctcgtcagc gcgaccgtca ggcacagcag
     2941 cccgaccaaa gcaagccggc atggcgccgc gcggcgatac ggcaggcata atcgcaggca
     3001 ggcgccgccc ggccgccggc cgggcaggcc tgtggtttcc cgggctgtcg aagtccgtcc
     3061 gattgctccg gcctccgcgc atggttccgg cgcatggctg caaggaaatc ccccacggcg
     3121 cacccttgat caggatacca gacgggtcaa gcgttgagaa gtccaggcgg ccgttttttc
     3181 gctaatttac gtccttcgtc cctgtgatcg actccgaagc cgcaccccgg ttttctctcc
     3241 tcaaagtgaa tcatagcgcg gcgaaccgcc ggttcagccc gcgtggcgag actgatcttc
     3301 aacttgtttt tggggcggtc tggcctatac ttgaggcaag gatcagcaat tcgcgcttgc
     3361 cgcgcctgga agaggacact gcgatgccgc cagccgctcg agtcaatgac cccgtcagtc
     3421 accccttgcc gccggtcctg aacccgggtc cgggcagccc cgatgtgaag atcggcttcc
     3481 tgcccgcctg gcggggggtt ccctccgcgg ccgcagcctc catccagtcg gccaaggcta
     3541 tcagcgatgc ggcgatcaag tcggcggagg ccgccaccgc cgccgccgcc gggacgcccg
     3601 gtctgccggc ggccaagacc gcggaagaaa ccaccaaggc caacgccgcg acgagcatgg
     3661 gcagcacgat cacttc
//
LOCUS       DAOPCO010000362         3668 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20447_length_3668_cov_3.756712, whole genome shotgun sequence.
ACCESSION   DAOPCO010000362 DAOPCO010000000
VERSION     DAOPCO010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20447_length_3668_cov_3.756712"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLS90_16330"
     CDS             complement(<1..244)
                     /locus_tag="PLS90_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPK04013.1"
                     /translation="MLSEEFLKPINMTQTQLAERIGVSYVRVNEIVNARRGITPDTAL
                     RLSTFGATPDFWLNGQLALGSLPHPTIARNPRRAQED"
     gene            complement(291..2732)
                     /locus_tag="PLS90_16335"
     CDS             complement(291..2732)
                     /locus_tag="PLS90_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPK04014.1"
                     /translation="MPPLPAADQSAIEDALASLQGSRRLRNNIALWHRIPAQPATYAP
                     FPDVLDPALADILRAQGIDQLYSHQAEAVARSLAGEHVVIVTPTASGKTLCYNLPVLQ
                     RLLRRPEARALYLFPTKALSHDQYGGLYDLTRALQRDIKVFTFDGDTPAAARRSIRSA
                     GQIVVTNPDMLHTGVLPHHTKWIKLFENLETVVIDELHQYRGVFGSHVANVIRRLRRI
                     CRFHGSDPRFICCSATVANPLEMAQTLVEAPFRLIDNNGAPRGERVVVFYNPPVVNQE
                     LGIRASSVKESRRVTLRFIERGLQTIVFARSRVRVEILTNYLKRAYAKMGGDPGRIRG
                     YRGGYLPNERREIERGIKNGEILCVVSTNALELGIDIGSLQAAVLCGYPGTIASAWQQ
                     GGRAGRKQQTAVMVVVGSSSPMDQYLMAHPEYFLGASPEHGILNPDNIMILSSHLKCA
                     VNEAPLRTDEPFGPAHPAALLDYLEEQRIVRKSGDRYHWSSDSYPAEGVSLRTATPQN
                     FIVHNKSDGNRVLAEVDYDSAQELIHPQAIYMHQGQTFYVDELDWDRRKAFVRLVKAD
                     YYTDAVSKSDLFVLHVDESEAWEEKSENPPANQRTENPSDPSDRTDRTDLVSISSPFS
                     PVLSRNFGEVRVDITVPKFKKVKFETQESIGMGDIDLPQYELHTQAAWWTFRPESQAW
                     LEARSLDLSSALKAIAWVVGNVAALHIMSDRRDMPALAMVRSTFDGLPALYVYDRFPG
                     GIGLARRAYGLDRQIFRAALEVIEACRCDHGCPACVGPPLEMGDRARAAARVLLRAMA
                     GKLSFGCKQNGRGHI"
     gene            complement(2943..3632)
                     /locus_tag="PLS90_16340"
     CDS             complement(2943..3632)
                     /locus_tag="PLS90_16340"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPK04015.1"
                     /translation="MRPPSPDPRAGLRGDLLFRWVVIPLVALLIRLIVFMQRRRYVGL
                     EHFRAAIDSGRPVVWAFWHEDLIGMAMTYLHLKSPRDVAIMISRSRDGDRLARLMQRL
                     GARPVRASSSRGAVRGFLEVLRRLRPGDAAPGTVAIALDGPRGPRRQPKPGAALLARK
                     AGALIVPVAFHHQRQWVFGSWDRTRLPKPFTRTTCVFGEPLDASRWDDEAALLQRMVE
                     ALDQMHGAAPV"
BASE COUNT          626 a         1018 c         1412 g          612 t
ORIGIN      
        1 gatcttcttg agcgcggcgc gggtttcggg cgatcgtcgg gtgtggtaga gatcccaacg
       61 cgagctggcc gttcagccag aagtccgggg ttgcaccgaa cgtggaaagg cgcagcgcgg
      121 tgtcaggcgt tataccacgg cgtgcattca cgatttcatt cacccgcaca taggaaacgc
      181 caatgcgctc cgcgagctgt gtctgagtca tgttgatcgg cttcaggaat tcctcagaca
      241 gcatcttgcc aggatgggtg gggggacgta tggatggaat gcgaagcatg tcagatgtgc
      301 cctcttccgt tttgtttgca accgaaggat aatttgcctg ccatggcccg cagcagcacg
      361 cgggcggcgg cgcgggcgcg gtcgcccatt tcgagcggcg gtccgacgca ggcggggcag
      421 ccgtggtcgc agcggcaggc ctcgatgacc tccagcgcgg cgcggaagat ctgacgatcc
      481 aggccgtagg cgcgccgggc caggccgatg ccgccgggga agcggtcgta gacgtagagc
      541 gccgggagcc cgtcgaacgt ggagcggacc atcgcgaggg ccggcatgtc gcgccggtcg
      601 ctcatgatgt ggagcgcggc gacgttgccc acgacccagg cgatcgcctt gagcgcgctg
      661 ctcagatcga ggcttcgggc ttcgagccag gcctgggact cggggcggaa cgtccaccag
      721 gcggcctggg tatgcagttc atactggggc aggtcgatgt cgcccatgcc gatcgattcc
      781 tgggtctcga acttgacctt cttgaatttg gggacggtga tatcgacgcg gacctcgccg
      841 aagttgcgcg agaggacggg cgaaaagggc gaggaaatcg aaacgagatc cgtccgatcg
      901 gtccgatccg acggatccga cggattttct gtacgctgat tggcaggggg attttcggat
      961 ttttcctccc aggcttcact ctcatcgaca tgcaggacga acaaatcgct cttgctgacc
     1021 gcgtcggtgt aatagtcggc cttgaccagg cggacgaacg ccttgcggcg gtcccagtcc
     1081 agttcgtcga cgtagaatgt ctggccctgg tgcatgtaga tcgcctgcgg gtggatcagt
     1141 tcctgggctg aatcgtaatc gacctcggcc agcacgcggt tgccatcgga cttgttgtgg
     1201 acgatgaaat tctggggcgt ggcggtgcgc agtgaaaccc cttccgccgg ataggaatcg
     1261 ctcgaccagt gataacggtc gcccgacttg cgcacgatgc gctgctcttc gaggtaatcg
     1321 agcagggcgg ccggatgcgc gggaccgaag ggttcatcgg tccgcagcgg cgcctcgttg
     1381 accgcgcatt tgagatgcga ggaaagtatc ataatgttat cgggattaag gatgccgtgc
     1441 tcgggcgagg cgccgaggaa atactcgggg tgggccatca gatactgatc catcggcgaa
     1501 gagctgccga cgacgaccat cacggcggtc tgctgcttgc gtccggcgcg tcccccctgc
     1561 tgccaggccg aggcgatcgt gccggggtag ccgcagagga cggcggcctg gagcgagccg
     1621 atgtcgatgc ctaattccaa tgcgttcgtc gagacgacgc agagaatctc gccgtttttg
     1681 atgccgcgct cgatttcgcg ccgttcgttg ggcagatagc ctccccgata gccgcggatg
     1741 cggccgggat caccgcccat cttcgcgtag gcccgcttga ggtagttggt gaggatttcg
     1801 acgcggacac gcgagcgggc gaagacgatg gtctgcaggc cgcgttcgat gaagcgcagc
     1861 gtgacgcggc gcgattcctt gacggagctg gcgcgaatgc cgagttcctg attgacgacc
     1921 gggggattgt agaacacgac gacgcgttcg ccgcgcgggg cgccgttgtt gtcgatcagg
     1981 cggaaggggg cttcgacgag cgtctgggcc atttcaaggg gattggcgac ggtcgccgag
     2041 cagcagatga agcggggatc ggagccgtgg aagcggcaga tgcgccgcaa gcggcggatc
     2101 acgttggcga cgtggctgcc gaagaccccg cggtactggt gcagctcgtc gatgaccacg
     2161 gtctcgaggt tttcgaacag cttgatccac ttggtgtgat gcgggaggac gccggtgtgg
     2221 agcatgtcgg ggttggtgac gacgatctgg ccggcggagc ggatcgagcg tctggcggcg
     2281 gcgggggtgt cgccgtcgaa ggtgaagacc ttgatgtcgc gctggagcgc gcgggtcagg
     2341 tcgtagaggc cgccgtactg atcgtgggag agcgctttgg tggggaagag gtagagcgcg
     2401 cgggcctcgg gccggcgcag caggcgctgg aggaccggga ggttgtaaca gagggttttg
     2461 cccgaggcgg tgggggtgac gatcacgaca tgctcgccgg cgagagaccg cgcgaccgct
     2521 tcggcctgat ggctgtagag ctgatcgatg ccctgggcgc gcaggatatc ggcgagggcc
     2581 gggtcgagga catcggggaa cggcgcgtag gtggccggct gggccgggat gcgatgccag
     2641 agggcgatgt tgttgcgcag gcggcggctg ccctgcaggc tggccagggc atcttcgatg
     2701 gcggattgat cggcggcggg cagggggggc atggtggagg ggaggacctc ggttcttcgc
     2761 aaaatcttcg cttgccagcc tagcgcagaa tgggtgttga atcgagggct tttgccgggg
     2821 ggcggggttg caagggaggg ggcgtaaagg agatagggga aagatgagac aggagggcag
     2881 aaccggcggg ggcgccggtc tcacaccggc tcagaccggc gaaggggcgg tctcacaccg
     2941 gctcagactg gcgccgcacc gtgcatctgg tccagcgcct caaccatgcg ctggaggagc
     3001 gccgcttcgt cgtcccagcg ggaggcgtcg agcggctcgc cgaacacgca ggtggtgcgt
     3061 gtgaacggtt tagggaggcg cgtgcggtcc caggagccga agacccactg gcgctggtgg
     3121 tggaaggcga cggggacgat gagggcgccg gccttgcggg cgaggagggc ggcgccgggc
     3181 ttgggctggc ggcgggggcc gcgcgggcca tccagcgcga tggcgaccgt gcccggcgcg
     3241 gcgtcgccgg ggcgcaggcg cctgaggact tcgaggaacc cgcggaccgc gccgcgcgag
     3301 gaagaggcgc ggaccggccg cgcgccgagc cgctgcatca accgggcgag gcggtcgccg
     3361 tcgcgcgagc ggctgatcat gatggccacg tcgcgcggcg acttcagatg cagataggtc
     3421 atcgccatgc caatcaggtc ctcgtgccag aacgcccaga cgaccggccg gccgctgtcg
     3481 atggcggcgc ggaaatgttc caagccgacg tagcgccggc gctgcatgaa gacgatcagg
     3541 cggatcagca gggcgacgag cgggatgacg acccagcgga agaggaggtc gccgcgtagg
     3601 ccggcgcggg ggtcaggact cggcggtcgc atcgggggca tccggctcgg gcggatggac
     3661 ggcaggga
//
LOCUS       DAOPCO010000363         3661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20497_length_3661_cov_4.414309, whole genome shotgun sequence.
ACCESSION   DAOPCO010000363 DAOPCO010000000
VERSION     DAOPCO010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20497_length_3661_cov_4.414309"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..770
                     /locus_tag="PLS90_16345"
     CDS             135..770
                     /locus_tag="PLS90_16345"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPK04016.1"
                     /translation="MARARYLTIRSDHPEFHRVGRVVSLLEQGGVVLIPTDTVHALVC
                     DPRRKEALARLRQIKPSLGDRPLTLMTRDLSVASRYAYLDDAAFKFIKGLTPGPYAFL
                     LKGTKEVPRLVLDPKRKQAGIRIPASPLCRALLEGLGGLLVSTSATLEAGAAPASHWD
                     VFEEYENKVDLIVDDEGELGTVPSTIIDMVSVPYRIAREGRGMAALAPIMA"
     gene            complement(828..1085)
                     /locus_tag="PLS90_16350"
     CDS             complement(828..1085)
                     /locus_tag="PLS90_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007619897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPK04017.1"
                     /translation="MSQFKPARRRIAVSVGESVRIIRELQELSQNQLSELTGIPQPTL
                     SAIENNRVNLGVERAKVLARALRCHPAVLLFPGWDAEQFAA"
     gene            complement(1089..1367)
                     /locus_tag="PLS90_16355"
     CDS             complement(1089..1367)
                     /locus_tag="PLS90_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HPK04018.1"
                     /translation="MWRIEEHRRVEKQLDAVPADILKRYEKWLDIATLSGPPGLRAIK
                     GFHDEALSGEWKGHRSSRLSLQDRVIYRVVTGEQRFEVLSVTAHEYRR"
     gene            1625..3019
                     /gene="hisD"
                     /locus_tag="PLS90_16360"
     CDS             1625..3019
                     /gene="hisD"
                     /locus_tag="PLS90_16360"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPK04019.1"
                     /translation="MQIWSWPRDRKAIAAHFAAVDETPRAIEAAVEAIIEDVRRDGDA
                     ALARYGRKFDKVDLKPGQFEVPRAALAAAWAAAPARLRRTLQTAKRRIEQFHRRQRLA
                     GWTVEEPGFGRIEQRVLPLERVGVYVPAGEAPLVSTVLMDVIPARVAGVDEICLFTPP
                     RRDGQPDGGILAAAHLAGVDRVFRLGSAWAVAAAAFGTETVPRVDKVVGPGNAYVTTA
                     KQRLFGRIDIESTAGVSEVLILADATARLEHIAADLLAQAEHSGDNPCGVILIGGDRK
                     RAEALRRELARRLGRLERGEMAARSIARHGYIILVKTAEEAIELANRKAPEHLEIIAR
                     GARRMARQVRHAGAIFVGPWTPEPLGDYLAGPNHTLPTGGTARFFSPLSVWSFYRTSH
                     TIEATREGLAKLADDIILLAEAEGLTAHAETIRARFEPGPEAGGKRGGKRAAPTAGKR
                     GAAGGKRAAKRADS"
     gene            3016..3336
                     /gene="sugE"
                     /locus_tag="PLS90_16365"
     CDS             3016..3336
                     /gene="sugE"
                     /locus_tag="PLS90_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008436485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HPK04020.1"
                     /translation="MTAWGWLVIAGLMEVVWAVGLKYTDGFRRPGPTALTLAAMAISF
                     YLLAVAVRTLPVGTAYAVWTGIGVLGTALVGVAALGEPATAARLGCLALIVGGIVGLR
                     LTAS"
     gene            3350..>3661
                     /locus_tag="PLS90_16370"
     CDS             3350..>3661
                     /locus_tag="PLS90_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018619863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPK04021.1"
                     /translation="MHRAPLLTRLRRHVPSDAVEAEHLQRMIEFVERENECFERSLAR
                     GHLTGSAFIIDEGRTHALLLHHAKLERWLQPGGYADGDQDLHAVALREAREETGLASI
                     QP"
BASE COUNT          572 a         1084 c         1425 g          580 t
ORIGIN      
        1 ttgttgggag ctgaggccgc tgatgcggag ttggcaaatt gacgatgagg ggttgtagga
       61 tagacggcgc cgcgtccggg ctggggacgc ggccgccaag caccaccacc gcaggcagga
      121 atcgaggtcg ggcgatggct cgcgcgcgct atctgacgat ccgcagcgat cacccggagt
      181 ttcaccgcgt cggccgcgtc gtatcgctgc tggagcaggg gggcgtcgtg ctgatcccga
      241 ccgatacggt gcatgcgctg gtgtgcgatc cgcggcgcaa ggaggcgctg gcgcggctgc
      301 ggcagatcaa accgagcctg ggcgaccggc cgttgacgct gatgacgcgc gatctgtcgg
      361 ttgcgtcgcg ctatgcctac ctggacgacg cggcgttcaa attcatcaag gggctgacgc
      421 ccgggccgta cgcgttcctg ctgaagggga ccaaggaggt gccgcggctg gtgctggatc
      481 cgaaacggaa gcaggcgggg atccggatcc cggcgtcgcc gctctgccgg gcgctgctgg
      541 aggggctggg gggtctgctg gtgagcacgt cggcgacgct ggaggcgggc gcggcgccgg
      601 ccagccactg ggatgtcttc gaggagtatg agaacaaggt ggatctgatc gtcgacgatg
      661 aaggggagct ggggacggtc ccctcgacga tcatcgacat ggtcagcgtt ccctaccgga
      721 tcgcgcggga ggggcggggg atggcggcgc tggcgccgat catggcgtag cggcctgacc
      781 cgtagcggcc tgacgatggg cggggtcccg catgttagag aagtcggtca agccgcgaac
      841 tgttcggcgt cccagccggg gaagagcaat accgccggat gacacctgag cgcgcgagcc
      901 agcaccttgg cgcgctcgac gccgaggttc acgcgattgt tttcgatagc cgagagggtc
      961 ggctgcggga tccccgtcaa ctcggacaac tggttctggc tcagctcctg caactcgcgg
     1021 atgatccgca ccgactcacc cacgctcacc gcgatgcgcc ttctggccgg tttgaactgg
     1081 ctcatcattc acctccggta ctcatgagcc gtgacggaga gcacctcgaa cctctgctcg
     1141 ccggtcacaa cgcgatagat aacccgatcc tgtaaggata aacgggatga cctgtgtccc
     1201 ttccactctc ccgacagtgc ttcatcatga aaccccttga tcgcgcgcag tccaggcgga
     1261 cctgagaggg tggcaatatc aagccatttt tcgtaacgct tgagaatgtc ggctggcacg
     1321 gcatcaagct gtttttccac gcgccggtgt tcctcaatgc gccacatatc ctattgtgcc
     1381 tatgtccaaa aggttatgtc aagccggttg ctcactcagt gaagtgtgtc ggggcgttcg
     1441 cccttgtgcc acgccatatc tcgacgatcc atccatcctt taggatggcg ccgacgtcgg
     1501 gccctcactt gcatgaaaga attccaagat gcgaactgta ttcctcgcgt gaggcttggc
     1561 tcttgtctgt cgggcggggg gtgacgcaga atggtcgttt gatcccgcgc cggaggaggg
     1621 ggcgatgcag atctggagtt ggccacgcga tcggaaggcg atcgccgcgc attttgcggc
     1681 ggtcgacgag accccgcgcg ccattgaggc ggcggtcgaa gcgatcatcg aggacgtgcg
     1741 gcgcgacggc gacgcggcgc tggcgcgcta tgggcgaaaa ttcgacaagg ttgacttgaa
     1801 accgggtcag ttcgaggtgc cgcgcgcggc gctggcggcg gcgtgggcgg cggcgccggc
     1861 ccggctgcgg cggacgctcc agacggccaa gcggcggatc gaacagttcc atcgccggca
     1921 gcggctggcg gggtggacgg tggaggaacc gggcttcggg cggatcgagc aacgcgtgct
     1981 gccgctcgaa cgggtgggcg tctacgtccc ggcgggcgag gcgccgctgg tctcgacggt
     2041 gctgatggac gtgatcccgg cgcgggtggc gggggtggac gagatctgcc tgttcacgcc
     2101 gccgcggcgc gacgggcagc ccgacggggg gatcctggcg gcggcgcacc tggcgggggt
     2161 cgaccgcgtc ttccggctgg ggagcgcctg ggccgtggcg gcggcggcgt tcggcaccga
     2221 aacggtgccg cgggtggaca aggtggtggg acccggcaac gcgtatgtga ccacggcgaa
     2281 gcagcggctg ttcgggcgga tcgacattga atcgacggcg ggggtaagcg aggtgctgat
     2341 tctggccgac gcgacggcgc ggctggagca catcgcggcg gatctactgg cgcaggccga
     2401 gcattcgggc gacaacccgt gcggcgtaat cctgatcggc ggggatcgga aacgggccga
     2461 ggcgctgcgg cgggagctgg cgcgccggct cgggcggctg gagcggggcg aaatggccgc
     2521 ccggtcgatc gcgcgccacg gctatatcat cctggtgaaa acggccgagg aggcgatcga
     2581 gctggccaac cgcaaggcgc cggagcatct cgaaatcatc gcgcgtggcg cgcgacgcat
     2641 ggccaggcag gtgcgccacg cgggggcaat ctttgtcggc ccgtggacgc ccgagccgct
     2701 gggcgattac ctggcggggc cgaatcatac gttgccgacg ggggggacgg cgcggttttt
     2761 ctcgccgctg agcgtctggt cgttctaccg gacgagccac acgatcgagg cgacgcgcga
     2821 ggggctggcg aagctggccg acgacatcat tctgctggcc gaggccgagg ggttgacggc
     2881 gcacgccgag acgatccgcg cgcggttcga accggggccg gaggccggcg gaaagcgggg
     2941 aggcaagcgg gccgcgccaa cggccggcaa gcggggggcg gcgggcggca aacgcgcggc
     3001 gaagagggcc gactcgtgac ggcgtggggc tggctcgtga tcgcggggct gatggaggtc
     3061 gtctgggccg tggggctgaa gtacaccgac ggtttccgcc ggccgggacc gacggcgctg
     3121 acgctggcgg cgatggcgat cagcttctat ctgctggcgg tggcggtgcg aacgctgccg
     3181 gtggggacgg cgtatgcggt gtggaccggg atcggcgtgc tggggacggc gctggtgggg
     3241 gtggcggcgc tgggcgagcc ggcgacggcg gcgcggctgg gctgcctggc gctgatcgtc
     3301 ggggggatcg tgggcctgcg gctgaccgcc tcgtgaggaa gggccgccga tgcaccgcgc
     3361 cccgctgctg acgcggctgc gccgccatgt tccgtccgac gcggtcgagg ccgaacacct
     3421 gcaacggatg atcgagttcg tggagcggga gaacgaatgc ttcgagcgtt cgctggcgcg
     3481 cgggcacctg accggctcgg cgttcatcat cgatgagggg cggacgcatg cgctgctgct
     3541 gcaccacgcc aaactggagc gctggctgca gccgggcggg tatgccgacg gcgatcagga
     3601 cctgcacgcg gtggcgctgc gcgaggcgcg cgaggagacc gggctggcgt cgatccagcc
     3661 g
//
LOCUS       DAOPCO010000364         3624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20712_length_3624_cov_5.190810, whole genome shotgun sequence.
ACCESSION   DAOPCO010000364 DAOPCO010000000
VERSION     DAOPCO010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_3624_cov_5.190810"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..722
                     /locus_tag="PLS90_16375"
     CDS             135..722
                     /locus_tag="PLS90_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPK04022.1"
                     /translation="MSALNPRGLTRAGAVRTAAVILLAVALLSTTGCSWFRRDKGVDV
                     DEQYIEDDTRTDLGAGSGATTGTVPSDAQAEGPRPGELLPFPELQTIYFDYDRSNIRA
                     DQVEKMQRNLQYLLDHPQDKVYIIGHCDERGTIEYNFNLGDRRAKEVAEFFRRGGVDA
                     SRIATVSRGEEEPVCTEHNESCWSRNRRAEFMRMY"
     gene            complement(858..2165)
                     /locus_tag="PLS90_16380"
     CDS             complement(858..2165)
                     /locus_tag="PLS90_16380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK04023.1"
                     /translation="MQQRRFFHPLAVAAGLALAAFLAACGQDISLTPTADGATQAPAT
                     LREKVREIQGLDAPVDVPVIELRRATIKETLSITGELAPRQSAMVKPMMEGRLTFLRR
                     IEVGTMVEEGETLAKIDDRDIEADIENQRKQVEISREKIELDRRDLLQKEKSFEFDKK
                     LKARNFITQIEFDRSELAVQQAQINLRQSQIMLEQEEKKLEKLLRQREKVPIVAPISG
                     MVVLASHLREQSTQSDLLNEEIMRLEDTLVGPSTELFGIISHDHFEARCMVNGRDKAR
                     LTPGQEVQARVIAHREITVPGRIRTVSRVQDQQSHAYKVWIELDEMDPAFTSGLFVRA
                     DVVLDQHEQALVVERDYLKERDGRPIVQIVRQGTVADMPVQLGLRNDKTVELLSGVNE
                     GDLLIASRDLYVPGQYVTPVELLARENPATDDEMTTLPAASAW"
     gene            complement(2239..>3624)
                     /gene="guaD"
                     /locus_tag="PLS90_16385"
     CDS             complement(2239..>3624)
                     /gene="guaD"
                     /locus_tag="PLS90_16385"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HPK04024.1"
                     /translation="RRESGPTSPPSKNSSAPASPAAPPIAALHLGELWHLTRRPRPGE
                     DPAAGLEVIARGALAVDPAGRIAGRGPADALRRRWPDAPVIDHGHALLLPGFVDAHLH
                     FPQLDIIGCWGEDLLGWLDRHVFPAEAAFADADLAARAAARFVTALLAAGVTAAAIFS
                     SSHLDATDRLLREIDARGLRAIVGKLSMDRHAPPPLLRPPHVDIPEIRQLIDRWHGRA
                     DRIQIALTPRFVPTCSPRLLRALGDLARERPDLPVQTHWAETEAEIDRVRRLHPRALD
                     YLELYEQAGLVRPRALLGHGVHPTPRELERLAPRGATVVHCPLANRFLGAGLFPLARY
                     RAAGVAVALGSDVGAGTSFSPWRVMLAAYEVQRRQGLTLHPVELLDLATLAGAAAIGL
                     EDQIGNFATGKRADFIVIDPSRHPLLAARANASRDPADRLFALIALGDERLTRATYLD
                     GNRAWAASTDE"
BASE COUNT          568 a         1094 c         1340 g          622 t
ORIGIN      
        1 aagcaggcga ttttgcttga aatttccgct gcacaggtta caactgttgc caactttgct
       61 cgtacatgag caaagctcgc tcggactggc ttgccgggcg aatgattcca cagaggccag
      121 gttaattaac agccatgagt gccttgaatc cacgtgggct tacccgcgcc ggcgcggtgc
      181 gtacggcggc ggtcatcctt ctggcggtcg cgctgctctc gaccaccggc tgctcctggt
      241 tccgccggga caagggtgtc gacgtcgatg agcagtacat cgaagacgat actcgcacgg
      301 acctcggcgc gggcagtggc gccacgacgg ggaccgtccc gagcgatgct caggccgagg
      361 gcccgcgtcc aggcgaactg ctgccgttcc cggagctgca gacgatctat ttcgattatg
      421 atcgctcgaa tatccgggcg gatcaggtcg aaaagatgca gcgcaacctg cagtatctgc
      481 tggatcatcc gcaggataag gtctacatca tcggccattg cgatgagcgc ggcacaattg
      541 agtacaattt caatctcggc gaccgccgtg cgaaggaagt tgccgagttc ttccgtcgcg
      601 gcggtgtgga cgccagccgc atcgccaccg tcagccgcgg cgaagaagag ccggtctgca
      661 ccgagcataa cgagagctgc tggtcccgga accggcgcgc cgaattcatg cggatgtatt
      721 aattcgcgcc atattttcgc aatttcaatc ccgggtggag gccgatttgg gccccacccg
      781 gtttttttta atggggtggc gggggcgcgg gtcccacacc tgtgatggtg caatcgatgg
      841 gcgtctaatt cctcgcgtta ccacgctgag gcggcgggga gggtggtcat ttcatcgtcg
      901 gttgcaggat tttcgcgggc gaggagctcc acgggggtca cgtactgacc cgggacatac
      961 aggtcgcgcg aggcgatcag caggtcgccc tcgttgacgc ccgagaggag ctcgacggtc
     1021 ttgtcattgc gcaggccgag ttggaccggc atgtcggcga cggtcccctg gcggacgatc
     1081 tggacgatcg gccggccgtc gcgctccttc aggtagtcgc gctcgaccac cagcgcctgt
     1141 tcatgctgat cgagaaccac gtcggcgcgg acgaagaggc cgctggtgaa cgccggatcc
     1201 atttcgtcca gctcgatcca gaccttgtag gcatggctct gctgatcctg gacgcgcgag
     1261 acggtgcgga tgcgtcccgg gacggtgatc tcgcggtggg cgatgacgcg agcctggacc
     1321 tcctggccgg gggtcagccg cgccttgtcg cggccgttga ccatgcagcg cgcctcgaag
     1381 tggtcgtggc tgatgatgcc gaacaattcg gtcgacgggc cgaccagcgt atcctccagc
     1441 cgcatgatct cctcgttgag caggtcgctc tgggtcgatt gttcgcggag gtggctggcc
     1501 aggacgacca tgccgctgat cggggcgacg atcggcacct tttcgcgctg gcgcaggagt
     1561 ttttcgagtt tcttttcttc ctgctcgagc atgatctggc tctggcgcag gttgatctgg
     1621 gcctgctgga cggccagttc ggagcggtcg aactcgatct gggtaatgaa gttccgggcc
     1681 ttgagtttct tgtcgaattc gaaacttttc tccttctgga ggagatcgcg gcggtcgagc
     1741 tcgatctttt cgcgcgagat ttcgacctgc ttgcgctgat tttcgatgtc ggcctcgatg
     1801 tcgcggtcat cgatcttggc cagcgtctcg ccttcctcga ccatcgtccc gacctcgatc
     1861 cggcggagga aggtgagacg cccctccatc atcggcttga ccatcgccga ctggcggggg
     1921 gccagttcgc cggtgatcga gagtgtctcc ttgatcgtgg cgcggcgcag ttcgatgacg
     1981 gggacatcga ccggggcatc caggccctgg atctcgcgca ccttttcgcg cagcgtcgcg
     2041 ggagcctgcg tcgcgccgtc ggccgtgggc gtcaggctga tgtcctggcc gcaggcggca
     2101 aggaacgccg ccagggccag ccccgcggcg accgccagag gatgaaagaa ccggcgctgt
     2161 tgcatgccag aacccccgcg tactatgtga cgatcttgcg cgattggttg caagagaatc
     2221 cgcgcggggg ccggggcgtc attcgtccgt tgaggcggcc caggcgcggt ttccgtcgag
     2281 ataggtcgcg cgggtgagcc gttcgtcgcc cagtgcgatg agggcgaaca gccggtcggc
     2341 ggggtcgcgg ctcgcgttgg cgcgcgcggc gagcagggga tggcgggagg gatcgatgac
     2401 gatgaaatcg gcgcgcttgc cggtggcgaa attgccgatc tggtcctcca ggccgatcgc
     2461 cgcggcgccg gccagcgtcg ccaggtcgag cagctcgacg gggtggagcg tgaggccctg
     2521 ccggcgctgg acctcatagg ccgcgagcat cacgcgccag ggagaaaacg aagtccccgc
     2581 gccgacatcc gagccgaggg cgacggccac ccccgccgcg cggtagcgcg ccagcgggaa
     2641 gaggcccgcg ccgaggaacc gattggccag cgggcaatgc acgacggtcg ctccgcgcgg
     2701 cgccagccgc tccagctcgc gcggcgtcgg gtgcacaccg tgcccgagca gtgcgcgggg
     2761 tcggacgagc ccggcctgct catagagttc gagatagtcc agcgcgcggg ggtggaggcg
     2821 gcgaacgcgg tcgatttcgg cctcggtttc ggcccagtgg gtctggaccg ggaggtcggg
     2881 gcgttcgcgg gcgaggtcgc ccagcgcgcg cagcaggcgg ggggaacagg tcgggacgaa
     2941 ccggggggtc agcgcgatct ggattcgatc ggcgcggccg tgccagcggt cgatgagttg
     3001 gcggatctcg gggatgtcga cgtgcggcgg gcgcaggagg ggcggcgggg cgtggcggtc
     3061 catgctcagc ttaccgacga tcgcccggag tccgcgcgcg tcgatctcgc ggaggagccg
     3121 gtcggtggcg tccaggtggg aggagctgaa gatcgcggcg gcggtgacgc cggcggccag
     3181 gagggccgtg acaaagcgcg ccgccgcccg ggcggcgagg tcggcgtcgg cgaaagccgc
     3241 ctcggcgggg aagacgtggc ggtcgagcca gccgaggagg tcctcgcccc agcagccgat
     3301 gatgtcgagc tgagggaaat gcaggtgagc atcgacgaag ccgggcagga ggagggcgtg
     3361 accgtggtca atcaccgggg cgtcgggcca gcggcggcgg agggcgtcgg cggggccgcg
     3421 cccggcgatc cggccggcgg ggtcgaccgc cagcgccccg cgcgcgatga cctccaggcc
     3481 ggcggccgga tcctcgcccg gccgcgggcg gcgcgtcaga tgccagagtt cgcccagatg
     3541 cagcgcggcg atggggggcg cagcggggga agcgggggct gatgaattct tggagggcgg
     3601 agacgtggga ccggattccc gtcg
//
LOCUS       DAOPCO010000365         3614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20785_length_3614_cov_5.357966, whole genome shotgun sequence.
ACCESSION   DAOPCO010000365 DAOPCO010000000
VERSION     DAOPCO010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20785_length_3614_cov_5.357966"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLS90_16390"
     CDS             complement(<1..366)
                     /locus_tag="PLS90_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HPK04025.1"
                     /translation="MRILFELAHPKHAHLFRYAILELQSRGHTIGITARRKDVTLRLL
                     DAWGFPYACLSDYPSGGILALGVELVIRDWRLWKFARRFKPDVFVARVGPAAATVGFL
                     MRKPVIVFEDTEGGTLQQKI"
     gene            complement(378..1364)
                     /locus_tag="PLS90_16395"
     CDS             complement(378..1364)
                     /locus_tag="PLS90_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK04026.1"
                     /translation="MKLIIQIPCYNEEATLPATLRDLPRRIDGIDAIEVLVINDGSKD
                     RTVEVARAHGVAHIVDFSRNQGLAAAFRAGLEAALAHGADIIVNTDADNQYRGDCIER
                     LVRPILANEAEMVIGDRGIWQHQEFGFVKKCLQALGSWVVSRFAGCRVADVTSGFRAY
                     SREAALRINVLSRYTYTHETVIQCGQAGMRIVSVPIEVNPKTRESRLFRSMWAYIRRS
                     ATTILRIYTLYRPLRAFFFAGLIPFALAFLIGLRFLIYYLQGQGGGHIQSLILCGVLF
                     NLSFMLFVLGIVADLIGANRKLLEEIIVSMRRDQLRLGTSNPDLAQIQHHSS"
     gene            complement(1642..3387)
                     /locus_tag="PLS90_16400"
     CDS             complement(1642..3387)
                     /locus_tag="PLS90_16400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPK04027.1"
                     /translation="MEQYRGILLNVWREACRHIELAESILTIGRILQEHLPPGRLRII
                     HFNPADGTLETLAAYPADDPEPRPRRARLSRGEMAAIRRWCEVGRVAPLPTARGGAAS
                     SLPAALREAAAAEGQGVLICPLRTGEGALGALILTLDAPGPPQERHRELLTALLEPLA
                     IAMENDLRLRELRALREAAESDKGALLRRLGRRDLSDTIVGADQGLRRVMARVEQVAP
                     SDLPVLIFGETGTGKELVARAIHQRSARHAGPVLRVNCGAIPPELIDSQLFGHERGAF
                     TGATDARRGWFERADRGTLFLDEIGELPLAAQSRLLRILQDGWLERVGGQRPIQVDVR
                     IVAATHRDLAGMVGTGAFREDLWYRLAVFPIYLPPLRERHDDIEALARHFAQRAAIRF
                     GLPPLELRPGDLELLLAYDWPGNVRELATVIDRAAILGDGRGLDVAGAMGVGQRPPRA
                     ASAGAAGGGVQPPGVGHGDGFTSPGGPDSSAGDPAARAGGLDSAGAGTGGFPAPGAGA
                     GGFPPAGAGEAGFLSLEEATRRHIEAALARTAGRVDGPFGAARLLGLNPNTLRSKMRK
                     AGIDAQRFRPTPPRD"
BASE COUNT          623 a         1331 c         1118 g          542 t
ORIGIN      
        1 gattttctgc tgcagggttc ccccttccgt atcctcaaac acaataaccg gcttgcgcat
       61 cagaaagcca accgtggccg cggcgggtcc gacccgcgcg acaaagacat ccggcttgaa
      121 cctgcgggcg aatttccata gacgccagtc acggatgacc agctcaaccc ccagagcaag
      181 aatcccgccg ctgggatagt ccgataaaca agcgtaaggg aatccccatg catccagcag
      241 gcgcagcgta acatcctttc gacgagctgt gataccaatc gtgtgcccgc gcgattggag
      301 ctcaaggatg gcatatctga acaaatgcgc atgctttgga tgcgcaagtt caaagagaat
      361 gcgcatgaaa ccctcgtcta ggagctatga tgctggattt gtgcaagatc aggattcgag
      421 gttccaagac gtagttgatc tcgccgcatc gaaacgatga tttcttccag cagcttccga
      481 ttggccccga tcagatcggc cacgattccc agcacgaaca acatgaaact gagattgaac
      541 agcacgccgc agagaatgag cgactggatg tggccgccgc cctgcccctg aaggtagtag
      601 atcaggaacc gcagcccgat caggaacgcc agcgcgaagg gaatcaagcc ggcgaaaaag
      661 aacgcacgga gcggacgata cagcgtataa atccgcagga tcgtcgtggc gctgcggcgg
      721 atgtaggccc acatcgaccg gaagagccgg ctttcgcgcg tcttcgggtt cacttcgatc
      781 ggcaccgaaa cgatccgcat cccggcctgc ccgcactgga tcacggtctc gtgcgtgtag
      841 gtgtagcggc tcagcacatt gatccgcagg gcggcttcgc ggctgtaggc gcggaacccg
      901 ctcgtcacgt ccgccacgcg gcagcccgcg aaccggctga cgacccacga accgagcgct
      961 tgcaggcatt tcttgacgaa cccgaactcc tgatgctgcc agataccgcg gtcgccgatc
     1021 accatctcgg cctcgttggc caggatcggc cggaccagcc gctcgatgca gtcgccgcga
     1081 tactggttgt cggcgtcggt gttgacgatg atgtcggcgc cgtgcgcgag cgcggcttcc
     1141 agcccggcgc ggaacgccgc cgccagcccc tgattgcggc tgaaatcgac gatgtgcgcg
     1201 acgccgtgcg cgcgggccac ctcgacggtg cggtccttcg acccgtcgtt gatgaccagc
     1261 acctcaatgg catcgatgcc gtcgatccgg cgcggcagat cgcgcagcgt cgccggcagc
     1321 gtcgcctcct cattgtaaca ggggatctgg atgatgagtt tcattcgcaa cgcacggccc
     1381 ggcggggcgg ccgccggcgc cgaccccgct cccaccgctc ctcccgcctc ccccgagtca
     1441 tgggacgccc gcggcggcgc taagcctaat cagcacaaag ccgcgccgtg gcatccggat
     1501 gacctgagcg ccgagaaaat aggaatcgca tatcttttcc cggggcgcaa gcaatttggc
     1561 ggcgggaccc cctcgcgccc tcgcgacgcg gctgcggccc gggacgccgg cccttgcgga
     1621 gccagccggg gcggcggcgg gtcagtcccg gggcggggtg gggcggaagc gctgggcgtc
     1681 gatgccggct ttgcgcatct tcgagcgcag cgtgttgggg ttgagcccca gcagccgcgc
     1741 cgcgccgaac ggcccgtcga cccggcccgc cgtccgcgcc agcgccgcct cgatatgacg
     1801 gcgcgtcgcc tcctcgagtg agaggaaccc ggcctccccc gctccagccg gtgggaaccc
     1861 gccggcccct gcccccggcg ccgggaaccc ccccgtcccc gcgccggccg aatccagtcc
     1921 gcccgcgcgc gccgcgggat cgccggcgct cgaatccggc ccgcccgggc ttgtgaatcc
     1981 atcgccgtgg cccacccccg gcggctgcac cccgcccccg gccgcccccg cactcgccgc
     2041 gcgcggcggc cgctggccga cacccatcgc accggccacg tccaaccccc gcccgtcgcc
     2101 cagaatcgcc gcgcggtcga tcaccgtcgc caactcgcgc acgttgcccg gccagtcgta
     2161 ggccagcagc agctccagat cgcccggccg cagctccagc ggcggcagcc cgaaccggat
     2221 cgccgcgcgc tgggcgaagt ggcgcgccag cgcctcgatg tcgtcgtggc gctcgcgcag
     2281 cggcggcagg tagatcggaa agaccgccag ccgataccac agatcctcgc ggaacgcacc
     2341 ggtgccgacc atgccggcca ggtcgcggtg cgtcgccgcg acgatccgca cgtcgacttg
     2401 gatcggccgc tggccgccga cgcgctccag ccagccatcc tgcaggatcc gcagcagccg
     2461 gctttgcgcc gccagcggca gctcgccgat ctcgtccagg aacagcgtgc cgcggtcggc
     2521 ccgctcaaac cagccccgcc gcgcgtcggt cgccccggtg aacgccccgc gctcatgacc
     2581 gaaaagctgc gaatcgatca gctcgggcgg aatcgccccg cagttgacgc gcagcaccgg
     2641 cccggcatgg cgcgccgagc gctgatggat cgcgcgcgca accagctcct tgcccgtccc
     2701 cgtctcgccg aagatcagca ccggcaggtc ggagggggcc acctgctcga cgcgcgccat
     2761 cacgcggcgc aggccctggt cggcgccgac gatcgtgtcg ctcaggtcgc gccgccccag
     2821 ccgccgcagc agcgccccct tgtccgactc ggcggcctcg cgcagcgcgc gcagctcgcg
     2881 cagccgcagg tcgttctcca tcgcgatggc cagcggctcg agcagcgccg tcagcagctc
     2941 gcgatgccgc tcctggggtg gccccggcgc gtccagcgtc agaatcagcg cccccagcgc
     3001 cccttcaccc gtgcgcagcg ggcagatcag caccccttgt ccctccgccg cggcagcctc
     3061 gcgcagagcg gcgggcagac tgctcgcggc gccgccccgt gcggtcggca atggggcgac
     3121 ccgccccacc tcgcaccagc ggcggatcgc ggccatttcc ccccggctca gccgcgcccg
     3181 ccgcggccgc ggctccggat cgtccgccgg gtaggcggcg agcgtctcca gcgtcccgtc
     3241 ggcggggttg aaatgaatga tccgcaggcg gcccggcggg agatgttctt gcaggatccg
     3301 gccgatcgtc aggatcgatt cggccaattc aatgtgccgg cacgcctcgc gccagacgtt
     3361 cagcagaatc ccgcggtatt gttccatcgc ctgtccccct cagaataatg atccatcgtc
     3421 ctcgtgctcg tgctcggccc cagtccaact tcgaaaccaa tggaccgttc catttagatg
     3481 atatctcgtt atatttcaag cattaaatcg ttaaatagaa tggatcacct ccacgacgca
     3541 acaccagttc accataaaac cttgtcaata cataacttaa catcgattcc acgaactggc
     3601 ccggtcctcg ccta
//
LOCUS       DAOPCO010000366         3599 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_20893_length_3599_cov_3.846219, whole genome shotgun sequence.
ACCESSION   DAOPCO010000366 DAOPCO010000000
VERSION     DAOPCO010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20893_length_3599_cov_3.846219"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLS90_16405"
     CDS             <1..580
                     /locus_tag="PLS90_16405"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HPK04028.1"
                     /translation="DVGYDRPTNLCFASLVEWDVPACRPLRTDTHVQPSTFPYVPGLL
                     SFREIPALLPLLRRLRRRPDLIIGDGQGIAHPRRIGLSSHIGVMLDQPSLGWAKSRLI
                     GTHDPLPPERGAAVALLDGDEQIGWVLRSRTACRPTYISPGHRVSLERALELARGLLG
                     PYRLCDPARLAHQLTRELLQRVRAEGPAAYVR"
     gene            708..1328
                     /locus_tag="PLS90_16410"
     CDS             708..1328
                     /locus_tag="PLS90_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04029.1"
                     /translation="MEPTAVQPDWFFLIAGIVLGLFGWMLYWAGLPIIGAVVGAGAIG
                     SLGYMASGFVSAAWAPLVFGGAGAVLGAFLGIALIRALQLYFFFISGAILGAALAYQF
                     TGTTALQGSSAAAIIIVVVGALVGGFVLLKARRFVVAIVTAAIGAMLVQTGLQSQNSL
                     VLVTSFVIFAAVQIGLVRRYVDQEAFDRRTRNRLRATETPDVRTID"
     gene            complement(1366..3240)
                     /locus_tag="PLS90_16415"
     CDS             complement(1366..3240)
                     /locus_tag="PLS90_16415"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPK04030.1"
                     /translation="MRVIAFLLCLVVVCCVLAGIVVELRGRESGPAELSLADEILADT
                     LPRVREIERQGDLLVDTTKALEQKARQLDERELALDRRQEEIAQAWDEVEASRAEVEA
                     RRKEIAQGQARLEQEQQRLNERQAAMEADHSQAAEEEAQALAEREAELAARERQLEEQ
                     SAELEQRAAALAQQEAELKQQRSELEAEQAAAAAAAADTAKNAILFIGDGMGPAIVSG
                     GRLMKGGPTARLALDAFSHTGYSRTYSTDGYVTDSAAGATALASGIKSYNGSIGVSDA
                     KVDPSGESRAVQKLTDVAVAAGKSVGIITTTRVTHATPACFYASVLSRGEEIAIAEQV
                     TTSSLSLLMGGGRSNFFPTNWIDVETSETGLRQDGRNLVAELQEQGWTYVESASQLEA
                     IDPSEPDQRVLALFDYDHMEYELEREQDRLGEPSLAEMVAFAIEFLERDPDGYFLMVE
                     AGRIDHAAHANQARRALGDFVALDEALSTALARAAEDTLIVVTADHDTGGLALNGYGP
                     HEAVAGTGILGNAYPNSEHPRGFLSWGTGPGGDAPARVPADAPNFQHRAAYRESGASH
                     SGVDVPVAAHGPGAARFTGYMENYLIPWKIAESLGLTFTEPANIENRRAWEARVGQGS
                     "
BASE COUNT          511 a         1401 c         1006 g          681 t
ORIGIN      
        1 tgacgtcggc tacgaccgcc cgacgaacct ctgtttcgcc tcgctggttg aatgggacgt
       61 gccggcatgt cggccgctcc ggaccgacac ccacgtccag ccgtccacgt tcccctacgt
      121 gcccgggctc ctcagcttcc gcgagatccc cgccttgctc cccctcctgc ggcggctccg
      181 gcgccggccc gacctgatca ttggcgacgg ccagggcatc gcccaccccc gccgcatcgg
      241 cctctcctcc cacatcggcg tcatgctcga tcagccgtcg ctcggctggg ccaagtcgcg
      301 cctgatcgga acgcacgacc cgctcccccc cgagcgcggc gcggccgtcg cgctgttgga
      361 cggcgacgag cagatcggct gggtcctccg ctcgcgcacc gcctgccggc cgacttacat
      421 ctcacccggt caccgcgtct cgctcgagcg cgcgctggag ctcgcccggg gtttgctcgg
      481 cccctaccgg ctctgcgatc cggcccgcct ggcccaccag ttgacgcgcg agttgctcca
      541 gcgggtccgc gccgaaggcc ccgcggcata tgtccgttga gaatgatccg ttttggtgca
      601 atcaataagg gtccgatcca ttgcgccgcg gccggcttcc gcaacccaaa ccgcaagccg
      661 gcccaccgga tccgcattcc atcactgacg cttctgggga tctcaccgtg gagccgaccg
      721 ccgttcagcc cgattggttc ttcctgatcg ccggcatcgt cctcggcctg ttcggctgga
      781 tgctctactg ggccgggctg ccgatcatcg gagcggtcgt cggcgccggc gcgatcggct
      841 cgctgggcta catggcttcg ggcttcgttt ccgccgcctg ggcgccgctg gtctttggcg
      901 gggccggagc ggtcctgggc gccttcctcg gcatcgccct catccgcgcc ctccagctct
      961 atttcttctt catcagcggc gcgatcctcg gcgccgccct cgcctaccag ttcaccggca
     1021 cgaccgccct ccagggctcc tccgccgccg cgatcatcat cgtggtcgtc ggggcgctcg
     1081 tcggcggctt cgtgctcctc aaggcacggc gcttcgtggt cgccatcgtc acggccgcca
     1141 tcggcgccat gctggttcag accggcctgc aatcgcagaa ttcacttgtg ctggtcacca
     1201 gcttcgtcat cttcgcggcg gtgcagatcg gcctggtgcg ccgctacgtc gatcaggaag
     1261 cgttcgatcg ccgcacccgc aaccgcctgc gcgccaccga aacccccgat gtccgcacga
     1321 tcgactgacg ccgattcaac cccgggcccc ctcgaattcg tgccctcacg acccctggcc
     1381 gacccgcgct tcccatgccc ggcggttctc gatgttcgcc ggctcggtga acgtcaaccc
     1441 caggctctcg gcgattttcc acgggatcag atagttttcc atgtatcccg taaatcgcgc
     1501 ggctcccggc ccatgcgcgg ccaccggcac atccacgccg ctatgggacg ctcccgattc
     1561 gcgatacgcc gcccggtgct ggaagttggg cgcatccgcc ggcacgcgcg ccggcgcgtc
     1621 cccgcccggt cccgtgcccc acgacaggaa tccccgcggg tgctcgctgt tcggatatgc
     1681 gttgcccaga atccccgtgc ccgccaccgc ctcgtggggc ccgtagccgt tcagcgccag
     1741 cccgcccgta tcgtgatccg ccgtcaccac gatcagcgta tcctccgccg cccgggccag
     1801 cgcggtggac agtgcctcat ccaacgccac gaagtcgccc agcgcccgtc gcgcctggtt
     1861 ggcatgcgcc gcgtgatcga tccgccccgc ctcgaccatc aggaagtagc cgtcggggtc
     1921 ccgctcgaga aactcgatcg cgaaggccac catctccgcc agcgacggct cgcccagcct
     1981 gtcctgctcg cgctccagtt cgtactccat atggtcgtag tcgaacagcg ccagaacccg
     2041 ctggtcgggt tcactggggt cgatcgcttc aagctgactc gccgattcca catatgtcca
     2101 accctgttcc tgaagctccg ccaccagatt ccgcccatcc tggcgcagcc ccgtttcgct
     2161 cgtttccacg tcgatccagt tcgtggggaa gaaattcgac cgcccgcccc ccatcaacag
     2221 cgacaggctg ctggtcgtca cctgttcggc gatggcgatc tcctcccctc ggctcagcac
     2281 cgaggcatag aaacacgccg gcgtggcgtg cgtcacccgc gtcgtggtga tgatccccac
     2341 cgacttgccc gccgccaccg cgacgtccgt cagcttttgc accgcccggc tttccccgct
     2401 cggatccacc ttcgcgtcgc tgacgccgat cgacccgtta tacgacttga tccccgacgc
     2461 cagcgccgtc gcccccgccg ccgaatcggt cacgtaacca tccgtcgagt acgtgcgcga
     2521 atagcctgtg tggctgaagg catcaagcgc cagccgcgcc gtcggtcctc ccttcatcag
     2581 ccgcccgccg ctgacgatcg cgggccccat cccgtcgccg atgaacagaa tcgcgttctt
     2641 cgcggtgtca gccgccgcgg ccgcggccgc cgcctgttcc gcctcaagct ccgaccgttg
     2701 ctgcttcagc tccgcttcct gctgcgccag cgcggcggcc cgctgttcca gctcggccga
     2761 ctgctcctcg agctgccgct cgcgcgccgc cagctccgcc tcccgctcgg ccagggcctg
     2821 cgcttcctct tccgccgctt gcgagtggtc ggcttccatc gccgcctgac gttcatttag
     2881 tcgctgctgc tcctgctcga gccgcgcctg cccctgcgcg atctccttgc gccgcgcctc
     2941 cacctccgcg cgcgaggcct ccacctcatc ccacgcctgc gcgatttcct cctgccgccg
     3001 gtccagcgcc aactcccgct cgtcaagctg ccgggccttc tgctccagcg ctttcgtcgt
     3061 atcgaccagc agatccccct ggcgttcaat ctcccgcacg cgcggcagcg tgtcggccag
     3121 gatctcatcg gcgagcgaca gttccgccgg tccgctttcg cggccgcgca gctcaaccac
     3181 gatccctgcc agaacgcagc acacgacgac gagacagagc agaaaggcaa tcacgcgcat
     3241 ggatggctac ctcggggttc cagtgattgg gcggcgggcc ggccgtttgc tcaccgatcc
     3301 aaactactgc ccgcccgcca cacggtcaag cgatgacatc tgaagtgctt tctatttgcc
     3361 gatctgaatc gcttccctcc tttgctccgc tcaggcatgg ggctgcattt ccgcctggcg
     3421 gcagtgggat caaaagatac aacggtcaca taatatcata aacgtacaca ttatccttga
     3481 taatcggacc tgaatctttc atagttactt ctcattgaga gaaagacatc caagtgagac
     3541 agcgattctg accatgacga cctcaagatc aacttcactt tgtgcgcttg catggatgt
//
LOCUS       DAOPCO010000367         3550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21234_length_3550_cov_4.541631, whole genome shotgun sequence.
ACCESSION   DAOPCO010000367 DAOPCO010000000
VERSION     DAOPCO010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21234_length_3550_cov_4.541631"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..2019
                     /locus_tag="PLS90_16420"
     CDS             166..2019
                     /locus_tag="PLS90_16420"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPK04031.1"
                     /translation="MNRTTLGVLLLMVGTLGHAAPAPGSPGPIELIPRPARLERQPGS
                     FVFSPGTRILAESGSAAARVAAQRLADLLRRAAGLELDVAESDAAEPVGGALLLTTRG
                     ARADLGPEGYELIVRPESIVIRAPGAAGLFHGAQSLRQLLPAAIESPRPVAGPFEWSV
                     PGLVIEDRPRFAWRGFLFDVGRHYYPREFIKRLIDLMAYYKVNTLHWHLTDDQGWRIE
                     IRRYPRLTEVGAWRAAAAPDSRRLGDGSAHGGFYTQDDIREIVDHARARGVTIVPEIE
                     MPGHALAALAAYPELSCTGGPFQVATEWGIYSDLFCAGNEAVFEFLEGVLTEVTELFP
                     GEYVHIGGDEAPKDRWRTCPRCQARIRSEGLADENALQGYFSGRINRILAARGRKLVG
                     WEEVIDGGFVPGATAMAWHLEGGDARAARAGHDVVMTPHLFTYLDYRESQLAGEPSPR
                     GWLPLRKLYEFEPVGKGLTREQAAHILGAQCCLWTEFFHAEAQVEHALLPRLSALAEL
                     TWSPAERKDWADYQARLAGHLRRLELGGVRFRIPTPEVAEIRAEGPRRRVSVALPFDG
                     AHVGYTLDGREPTFDSPRWRGPIELETSATLKLRTFLPGGRGSQVVEAHIP"
     gene            2103..3335
                     /locus_tag="PLS90_16425"
     CDS             2103..3335
                     /locus_tag="PLS90_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HPK04032.1"
                     /translation="MSKTVRAQLSVLMFFQFFGWGAWTVTMGTYLGRIGFQGIDIGNA
                     YSTTAWAAIISPFFVGMVADRFFSAQKVLGVLHILGGVTLFLASRMQTPAAFFWVLLL
                     HALCFMPTLALINAVSFHQMEDPSREFPGIRVLGTIGWIVAGWVIGLLRIEPTAIPLR
                     IAAANAVLLGLFCFTLPPTPPRSAGRRVAVRDILGLDALGLMKDRAFAVFVVSSLLIC
                     IPLAFYYNFTNPFLNESGMVNAAGKMTFGQMSEIFFMLVMPFFFARLGVKWMLIIGMA
                     AWTARYFLFAYGDNGALVWMFYLGILLHGICYDFFFVTGQIYVDQKAPPALRSSAQGF
                     LAFVTLGVGMLIGAKVSGWVVQHHQLMDGAQAVISHDWRRIWLVPAVMAGVVLVLFGA
                     LFNERRKGAQQATAMEAR"
BASE COUNT          499 a         1244 c         1167 g          640 t
ORIGIN      
        1 cgttcgatcc ggccgcgttc gacctgagca acacccggcc gctgctgatc ggcttcggcc
       61 aggagaacta cttcaacggg atgatccgcg acgtgaagct ctacaaccgc gcgttgtccg
      121 acgcgcagtt gcggcaaggg tcatagcggc cgacgggggg agacgatgaa ccggaccacg
      181 ctgggggtcc tgctgctgat ggtggggacg cttggccacg ccgcgccggc gccgggttcg
      241 cccggcccga tcgagctcat cccccggccc gctcggctgg agcgccagcc cggttcgttc
      301 gtcttcagtc ccggcacgcg gattctggcc gaatccggtt cggcggcggc gcgagtggcc
      361 gcgcagcggc tggccgacct gttgcgccgg gccgccggat tggagctcga cgtggccgaa
      421 tcggacgcgg ccgagccggt cggcggcgcg ctcctattga ccacccgcgg cgcccgggcc
      481 gatctcgggc cggagggcta cgagctgatc gtccggcccg aatcgatcgt gatccgggcg
      541 ccgggggccg ccggtttgtt ccacggcgca cagtcgttgc gccaactcct ccccgccgca
      601 atcgaaagcc cccgcccggt cgcgggcccg ttcgaatggt ccgttccggg cctcgtcatc
      661 gaggatcgcc cgcgcttcgc ctggcgcggc ttcctgttcg atgtcggccg ccactattac
      721 cccagggagt tcatcaagcg gctcatcgac ctgatggcct attacaaggt gaacaccctc
      781 cactggcacc tgaccgacga ccagggctgg cggatcgaga tccgacgcta cccgcggctg
      841 acggaagtcg gagcgtggcg cgcggcagcg gcccccgatt cgcgccggct gggcgatggc
      901 tcggcccacg gcgggttcta cacgcaagac gatatccgcg agatcgtcga ccatgcccgc
      961 gcccgggggg tcacgatcgt cccggagatc gagatgccgg gtcacgcgct cgccgcgctc
     1021 gccgcctacc ccgagttgtc gtgcaccggc ggcccgttcc aggtcgccac cgaatggggg
     1081 atttactccg acctgttctg cgccggcaac gaggccgtct tcgagtttct cgaaggggtc
     1141 ctcaccgagg tcaccgaact ctttccgggc gagtatgttc acatcggcgg ggacgaagcc
     1201 cccaaggacc gctggcgcac gtgcccgcgg tgccaggcgc ggatccggag cgagggcctg
     1261 gccgacgaaa acgccctgca gggctacttc tccggccgga tcaaccgtat cctggccgcc
     1321 cgcggccgca agctcgtcgg ctgggaggaa gtcattgatg ggggattcgt ccccggcgcg
     1381 acggcgatgg catggcacct cgaggggggc gacgcgcggg ccgcgcgcgc cggccacgac
     1441 gtcgtcatga cgccccacct gttcacctat ctggattatc gcgaatcgca actggccggc
     1501 gagcccagcc cgcgcggctg gctgccgctg cgcaaactct acgagttcga gccggtcggc
     1561 aaagggttga cccgcgaaca ggcggcgcat attctgggcg cgcagtgctg cctctggacc
     1621 gagtttttcc acgccgaggc gcaggtcgag catgcgcttc tcccccggct gtcggcgctg
     1681 gccgagctga cctggtcgcc cgccgagcgc aaggattggg cggactacca ggcgcgactg
     1741 gccggtcacc tgcgccggct cgagctgggg ggcgtccggt ttcgcatccc gacgccggag
     1801 gtcgctgaaa tccgggccga agggccccgg cgccgcgtga gcgtcgcgtt gccgttcgac
     1861 ggcgcgcacg tcggctatac gctcgacggc cgcgagccaa cgttcgattc gccccgctgg
     1921 cgcggaccga tcgagctgga aaccagcgcg acgctcaaac tgcggacgtt tctgccgggt
     1981 ggtcgcggca gccaggtggt cgaggcccac atcccatgaa cccccgcccc ccccggcatc
     2041 aaccgccgtt tctggaatga ccgaccctca ctctcgcgat ggaaggcgat ccccccatgg
     2101 ccatgagcaa aaccgttcgc gcccagctct ccgtcctgat gttcttccag ttcttcggct
     2161 ggggagcgtg gaccgtcacg atgggcacct acctgggccg gatcggcttt caggggatcg
     2221 acatcggcaa cgcctacagc acgaccgcct gggccgcgat catctcgccg tttttcgtcg
     2281 gcatggtcgc cgaccgcttc ttctccgccc agaaggtcct cggcgtgctc cacatcctgg
     2341 gcggcgtcac gctgttcctc gcctcacgca tgcagacccc ggcggcgttc ttctgggtgc
     2401 tgctgctgca cgcgctgtgc ttcatgccga cgctggcgct gatcaacgcc gtctcgttcc
     2461 accagatgga ggaccccagc cgcgagttcc cgggcattcg cgtgctgggg acgatcggct
     2521 ggatcgtcgc cggctgggtg atcggcctgc tgcggatcga gccgacggcg atcccgctgc
     2581 gcatcgccgc cgccaacgcc gtgctgctcg ggctgttctg cttcacattg ccgccgacgc
     2641 cgccgcgctc ggccggccgg cgcgtcgccg tgcgcgacat cctcgggctg gacgcgcttg
     2701 gcctgatgaa ggatcgcgcc ttcgccgtgt tcgtcgtcag ttcgctgctg atctgcatcc
     2761 cgctggcgtt ctattacaat ttcaccaatc cgttcctgaa tgaaagcggg atggtcaacg
     2821 ccgccggcaa gatgacgttc ggccagatgt cggagatttt cttcatgctg gtcatgccgt
     2881 tctttttcgc ccgcctcggc gtcaagtgga tgctgatcat cggcatggcc gcctggaccg
     2941 cccgttactt cctcttcgct tacggcgaca acggggcgct ggtctggatg ttctacctcg
     3001 gcatcctgct ccacggcatc tgctacgatt tcttcttcgt cacgggccag atttatgtcg
     3061 atcagaaggc gccgccggcg ctgcgctcga gcgcgcaggg gttcctcgcc ttcgtgacgc
     3121 tgggcgtcgg catgctgatc ggcgcgaagg tctccggctg ggttgttcag caccaccagc
     3181 tcatggacgg cgcccaggcg gttatcagcc acgactggcg gcggatctgg ctcgtcccgg
     3241 cggtgatggc cggcgtcgtg ctggttctgt tcggcgcgct gttcaacgag cggcgaaaag
     3301 gtgcacaaca ggcaaccgcg atggaagcga gatagacgga agggacataa cgcgagcccc
     3361 gtcgggattc gtccctcgtc tctcttccct cgtcctcgtc ctcgtgatcg cccccaaagg
     3421 cccgcccaga atccggctct cggctctcgt cctcggctct cgagcatccc ccgccggccc
     3481 gccccctctt cacatcaccc gttttgcgcc cattgttttt cggatcgggg gcgtagtaga
     3541 ctgggggggc
//
LOCUS       DAOPCO010000368         3542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21286_length_3542_cov_4.760539, whole genome shotgun sequence.
ACCESSION   DAOPCO010000368 DAOPCO010000000
VERSION     DAOPCO010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21286_length_3542_cov_4.760539"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            388..1689
                     /locus_tag="PLS90_16430"
     CDS             388..1689
                     /locus_tag="PLS90_16430"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018977469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPK04033.1"
                     /translation="MNVTVIGTGYVGLVTGVVFADLGNDVICVDKDDSKVERLSRGEP
                     TIYEPGLEEMLARNLNDGRLAFTSDLDSAVQKSDVIFIAVGTPPKEDGETDLSQVQAA
                     AEGIARAMDRYKVVVNKSTVPVGTGNFVRDIITRNQRRKVDFDVVSNPEFLREGSAIQ
                     DTLAPDRIVIGAPSQAVALTLLELYAPLEHPMLITDVESAEMIKYASNAFLATKISFI
                     NAVANLCEKVGADVVQVAKGMGYDKRIGAAFLQAGLGFGGSCFPKDTLSLIHTARKFD
                     EDNELLRAVVRINDQRVPRFCRRIEERLGALDGRVIGLLGLAFKPNTDDLREAKSLEI
                     TRCLLERGARVQAHDPVAMDGAKALIPELELTDNAYDAADGADALVIVTEWNEYKLLN
                     LERLKSLLTQPVVFDGRNIYSPERMRQLGFEYYAIGRGTIK"
     gene            1686..2780
                     /locus_tag="PLS90_16435"
     CDS             1686..2780
                     /locus_tag="PLS90_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPK04034.1"
                     /translation="MSDRLENLLNEMIERGSSDMFLKAGSPPYIRIDGEIISLDYEDL
                     TPEDTLEFARSSMTDNQWRSFEEVPEMDLAIGLSGVGRFRVNVFRQRGTIGMVLRHIN
                     NPEFSFQELNLPPVVKQLAEHKRGMTLVTGMTGAGKSTTLAAMINHINETRRCHIVTI
                     EDPIEFLHPDKKAVINQREVGFDTMGFADALKHVLRQSPDVILIGELRDLETIQTALS
                     AAETGHHVLSTLHTTDANQTVERMINYFPAYLHNQVRLELALSLNGIVCQRLLPRRVG
                     KGRVPALEIMINTPTIKKLLMEGRTTQIVEHIQDGEYYGMQTFNQSLLKLIQSNLIEY
                     EVALQYATSPEELRLAYQGMGADKLGTGTF"
     gene            3258..>3542
                     /locus_tag="PLS90_16440"
     CDS             3258..>3542
                     /locus_tag="PLS90_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPK04035.1"
                     /translation="MKWRTGRRSANVEDRRGRRPVGRMGGVPMVGGGMGLLLLLGISL
                     LAGLNPLEVLTLMSGQGGGPATYEQVEVDPAQAETAEFVSVVLADTEDVWH"
BASE COUNT          679 a         1281 c          980 g          602 t
ORIGIN      
        1 ctcggtggtt tcaaatgctc tcggttcgcg cgccatcttc ccggatcctt taacccttat
       61 tcacgtgacc accgcacaac gcggctatct ggaatctgac gcgtcccccc gcccaattgt
      121 tccgctccct cgcggccgga cgcgcggaag ggtcatacat caacaaaagg gatataaaag
      181 tcacaggccc gtccgccctt tcgtgcccct gcgtctctat tgtgatccag atcccccgcg
      241 tcctccatcg tgttgcaact tgccaacgga ttcgggtagc ctgtttcggc ttgcgggtcg
      301 ggggttgatc cgccggccgc cccgccctgc atgggcccgc ttcggtcccg gggcggtcat
      361 tatctgttcc cagggagctt ccgcgcaatg aacgtgaccg taatcgggac cggctatgtc
      421 ggtctggtga caggggtcgt tttcgccgat ctcggcaacg acgtgatctg cgtcgacaag
      481 gacgattcca aagtcgagcg gctcagccgc ggcgaaccga cgatctatga gcccggcctc
      541 gaggaaatgc tcgcccgcaa cctcaacgac ggccgcctcg ccttcacctc ggacctcgac
      601 agcgccgtcc agaagagcga cgtgattttc atcgccgtcg gcaccccccc caaggaggac
      661 ggcgagaccg acctgagcca ggtccaggcc gccgccgagg ggatcgcccg cgccatggac
      721 cgctataaag tagtggtcaa caagagcacc gtcccggtcg gcaccggcaa cttcgtccgc
      781 gacatcatca cgcgcaacca gcggcgcaag gtcgacttcg acgtcgtctc caaccccgaa
      841 ttcctccgcg aaggctccgc catccaggac acgttggccc ccgaccgcat cgtgatcggc
      901 gccccgagcc aggctgtcgc cttgacgctc ctggagttat acgccccgct ggagcacccc
      961 atgctgatca ccgacgtcga aagcgccgag atgatcaagt acgcctccaa tgccttcctg
     1021 gccaccaaaa tctcgttcat caacgccgtc gccaacctct gcgaaaaggt cggcgccgac
     1081 gtcgttcagg tcgccaaggg gatgggctac gacaagcgca tcggcgccgc cttcctccag
     1141 gccggcctcg gcttcggcgg ctcctgcttc cccaaggaca ctttatccct aatccacacc
     1201 gcgcggaagt tcgatgagga taatgagctg ctgcgggccg tggtgcgcat caacgaccag
     1261 cgcgtgccgc ggttctgccg gcggatcgaa gagcggctcg gcgcgctcga cggccgggtc
     1321 atcggcctgc tgggcctggc gttcaagccc aacaccgacg acctgcgcga ggccaaaagc
     1381 ctcgaaatca cgcgctgcct gctcgagcgc ggggcgcgcg tccaggcgca cgaccccgtg
     1441 gcgatggacg gcgccaaggc cctcattccg gaactggagc tgaccgacaa cgcctacgac
     1501 gccgccgacg gcgccgacgc gctggtcatc gtcacggaat ggaacgagta taaactgctc
     1561 aacctggagc gcctcaagag cctgctcacg cagccggtcg tcttcgacgg ccgcaacatt
     1621 tattcgcccg agcgcatgcg ccagctcggc ttcgagtatt acgccatcgg caggggaacg
     1681 atcaaatgag cgaccggctg gaaaacctcc tcaacgaaat gatcgaacgg ggctcctcgg
     1741 acatgtttct caaggcgggg agcccgccct atatccgcat cgacggcgaa atcatctccc
     1801 tggactacga ggacctgacg ccggaggaca ccctcgagtt cgcccgcagc tcgatgaccg
     1861 acaaccagtg gcgcagcttc gaggaagtcc ccgagatgga cctcgcgatc ggcctgtcgg
     1921 gtgtcggccg cttccgcgtc aatgtcttcc gccagcgcgg caccatcggc atggtcctgc
     1981 gccacatcaa caaccccgag ttctcgttcc aggaactgaa ccttcccccc gtcgtcaagc
     2041 aactggctga gcacaagcgc ggcatgaccc tcgtcaccgg catgaccggc gccggcaagt
     2101 cgaccactct cgccgcgatg attaaccaca tcaacgagac ccgccgctgc cacatcgtca
     2161 ccatcgagga cccgatcgag ttcctccacc ccgacaagaa ggccgtcatc aatcagcgcg
     2221 aggtcggctt cgacacgatg ggcttcgccg acgcgctcaa gcacgtcctg cgccagagcc
     2281 ccgacgtcat cctcatcggc gagctgcgcg acctggaaac gatccagacc gccctgtcgg
     2341 ccgccgagac cggccaccat gtcctctcga cgctccacac caccgacgcc aaccagaccg
     2401 tcgagcgtat gatcaactat ttccccgctt atctgcacaa tcaggtgcgc ctggagctcg
     2461 ccctcagcct caacggtatc gtctgccagc gcctgctgcc gcgccgcgtc ggcaagggac
     2521 gcgtccccgc cctcgaaatc atgatcaaca cgcccaccat caagaaactc ctgatggagg
     2581 ggcgcaccac ccagatcgtc gagcacatcc aggacggcga atactacggc atgcagacct
     2641 tcaaccagtc gctgctcaag ctgatccagt ccaatttgat cgagtacgag gtggccctgc
     2701 agtacgccac cagccccgag gaactccgcc tcgcctatca gggcatgggc gccgacaaac
     2761 tcggcaccgg caccttctga ccccgccccg gccccaccat cctgatgcat gcccgcgtaa
     2821 gaggcgggat tggatctcga ggcccatttg ttctccgtga acctccttca caatccccca
     2881 cccgcaagct tcgccgaaga tattctgaaa accggtctcc ggctggaaat ttagtcgctg
     2941 gagtccggac atccacaccc atctgtttta atccaaaaca atcgagcacc gccccctgca
     3001 tttcatttcg cttcgtgatg ccatcgcgtc cgcttcacca ggccggccgc cgcgaatgcc
     3061 ggcgcctccg aaagccgccc agctgactca acggaaaaag tccggacacc gggcaaccaa
     3121 gcgatcgatt cgatcccccg atcgcccgcc ccccccacca aatcatttcc cgaatcgatt
     3181 tccggccggt tttgtgctat cacgatcctg cctttcccgg ccgtcgcggc tcatcctgag
     3241 aaccgagtgc acccgacatg aaatggcgaa ccggtcgtcg cagtgccaac gtcgaggatc
     3301 ggcgcggacg gcggccggtc gggcgcatgg gcggcgtccc gatggtcggc ggcggcatgg
     3361 gcctgctcct cctgctcggc atcagcctgc tcgcggggct caacccactt gaggtcctga
     3421 ccctcatgtc cggccagggc ggcgggcccg ccacctacga acaggtcgag gtcgatcccg
     3481 cccaggccga aaccgccgag ttcgtctcgg tcgtgctcgc cgataccgag gatgtctggc
     3541 at
//
LOCUS       DAOPCO010000369         3530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21379_length_3530_cov_5.764604, whole genome shotgun sequence.
ACCESSION   DAOPCO010000369 DAOPCO010000000
VERSION     DAOPCO010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21379_length_3530_cov_5.764604"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..972)
                     /locus_tag="PLS90_16445"
     CDS             complement(256..972)
                     /locus_tag="PLS90_16445"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK04036.1"
                     /translation="MKLSPAARSFFRYQGFLRLPIGLEADVVTRLRETIQRHIREEIE
                     PYRRDGHGRIVRISNVWDRDPIFQETFTRPDLLDCLADLLGPNIEFMKNRHNHATLRL
                     RGETGDNDRPLLHRDALQWSRTIVSVVFYLEETTLENGCTHVVPGTQLLPITTSASMD
                     VEAGEYDAQLLEQLAPLPMPRGGIAVIDGMLIHGVGVNRTESSRMSMTAGYHSVDEMV
                     NEAPGHREVVRGSRFYLGNA"
     gene            complement(969..>3530)
                     /locus_tag="PLS90_16450"
     CDS             complement(969..>3530)
                     /locus_tag="PLS90_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK04037.1"
                     /translation="FLLGRRLTLQTAEGETLWETETIRHTSLLAAEDVDGDGRKELLL
                     TGYPPQLVVVSPEDGAMLSRIDLHPQATHITYWRLERLLPDAAGEQFLIYLKREDDKG
                     DVAGGWIALVDFSQGARDAGFLWQREIHLAAYPKTYMADANGDGVLEVIAQGHQEISA
                     LDARTGETIAHLKFGSGRRNYGFIEAVNLDEEPDDELVILTDESYPHMEVVKLGERPR
                     VLWYRSLVEENYPDEWRARYEALPTSVGDFNADGRIDMAYSIWSHAQGDRWHVRVDDA
                     RTSGVIAERPGYFLRGIERLTADGPALFLMSREPPDERGIRAAEGYRLEICRLTSDGF
                     ETLARVDDAGQLVTTGHELTLRQYSNTRSRPRPKLVAAEGGEAFLWKSAAGETAYSLS
                     ESNELVARASPPVPTPVGGPPRWQPPQHGNATPVIAADVTGDGVAEILLTRGDALVTL
                     QPAAGEPWREVWRFAPVKPGNVYFSVLALDLEGDGRHEVLAELADEGPGAKRLGLIDP
                     DGTLIREFRFEELKNPADSLKAWSTGDVNGDGRPDLIASLNLQTGSAMGKAGEPTVAV
                     DLATGERLWTTDRLSGTSWQGGDVRNTPFMAPTVPVITFRDLNGDGAEEVLYGCNYFN
                     FTVLNGPDGRPLDVYHVSNFIGEANSGYGFPVLADLDGDGRLEGLIEYGGFYGCAGAF
                     TLDFDAPPAKRKLWGESYNSRNGVYMLPQRYMLPQHYMTYGIGDLDGDERAELLMPHV
                     TEEMICRDGMTGRVLWRHAVGELPSTDVLVWDADGDGRDAFLFGTADGRLVALGAGGA
                     VQWETTLPATRDGAVSQVIAADVLERGAPQLIAVADGRVQVLAAAAGELAAEAHR"
BASE COUNT          527 a         1316 c         1074 g          613 t
ORIGIN      
        1 ctcgcagggg cgcgagctgt gaacggcgag ttcgtgaagc ttgtcggcga gatgttcgaa
       61 atcggtgaac caataggcgc ggtctacgct catggggcct ggccttcgca aattcccggc
      121 ggcggccgcc gcgccctggc cgagacccgc gcgcattcct cgcggccgtt tgccgcgtgc
      181 cccggttacg ggcatcactc ccgctgattc tatgggctga cgtccggttt tcactactga
      241 aatccttgct ccggttcagg cgttccccag gtaaaaacgg ctcccccgga ccacctcgcg
      301 gtgccccggc gcctcgttca ccatctcatc gacgctgtgg tagccggccg tcatgctcat
      361 ccggctcgat tcggtccggt tgaccccgac tccgtggatc agcatgccgt cgatcacggc
      421 gatgcccccc cgcggcatcg gcagcggcgc caattgctcc agcagttgcg cgtcgtattc
      481 gcccgcctcc acgtccatcg atgccgacgt cgtgatcggc agcagctgcg tgccgggcac
      541 gacgtgcgtg cagccgttct ccagcgtcgt ttcctccaga taaaagacga cgctgacgat
      601 cgtgcgcgac cactgcagcg cgtcgcggtg cagcagcggc cggtcgttat ccccggtttc
      661 cccccgcagc cgcagcgtcg cgtggttgtg ccggttcttc atgaattcga tgttcggccc
      721 cagcagatcc gccagacagt ccagcagatc gggccgggtg aacgtctcct ggaagatcgg
      781 gtcgcggtcc cacacattgg aaatccgcac gatccggccg tgcccgtcgc gccggtaggg
      841 ttcgatctcc tcgcggatgt ggcgctggat cgtctcgcgc aggcgcgtca ccacgtcggc
      901 ctccagcccg atcggcagcc gcagaaagcc ctggtagcga aagaagctgc gcgcggcggg
      961 gctcagcttc atcgatgggc ctccgcggca agttccccgg ctgcggcagc gagcacctgc
     1021 acccggccat cggcgacggc gatcaactgc ggcgcgcccc gttccagcac gtccgcggcg
     1081 atcacctggc tgaccgcgcc gtcgcgcgtg gccggcagcg tcgtctccca ttgcacggct
     1141 cccccggccc ccagcgccac gagccgtccg tcggccgtgc cgaacaggaa cgcatcgcgg
     1201 ccgtcgccgt cggcatccca caccagcacg tcggttgagg gcagctcgcc caccgcatgg
     1261 cgccatagaa cccggccggt catcccgtcg cggcagatca tctcctccgt cacgtgcggc
     1321 atcaggagtt ccgcccgctc gtctccgtcc agatccccga ttccgtaggt catgtaatgc
     1381 tgcggcagca tgtagcgctg cggcagcatg taaacgccgt tgcggctgtt ataggattcg
     1441 ccccacagtt tgcgcttggc ggggggcgca tcgaaatcca gcgtgaacgc gccggcgcag
     1501 ccgtagaacc cgccatactc gatcagcccc tccagacggc cgtcgccgtc cagatcggcc
     1561 agcaccggaa atccgtatcc gctgttggct tcgccgatga aattggagac gtgatagacg
     1621 tccagcggcc ggccgtcggg tccgttcagc accgtgaagt tgaaatagtt gcagccgtag
     1681 aggacctctt cggccccatc gccgttcagg tcccgaaacg tgatcaccgg gacggtcggc
     1741 gccatgaacg gcgtattccg cacgtccccc ccctgccaac tggtgccgct cagccggtcg
     1801 gtcgtccaca gccgctcgcc ggtcgccagg tccaccgcca ctgtcggctc gccggccttg
     1861 cccatcgcgc tgccggtttg caggttcagc gaggcgatca ggtcgggccg gccgtcgccg
     1921 ttgacgtcgc ccgtgctcca ggccttcagc gaatcggccg gattcttcag ttcctcaaac
     1981 cggaattccc ggatcagcgt cccgtccgga tcgatcagcc ccagccgttt ggcccccgga
     2041 ccctcatcgg ccagttcggc caggacctca tgacggccat ccccctccag atcgagcgcc
     2101 aggaccgaga agtagacgtt gcccggtttg accggcgcaa agcgccacac ttcgcgccac
     2161 ggttcgcccg cggccggttg cagcgtcacc agcgcgtccc cccgcgtcag caggatctcg
     2221 gcgacgccgt cgcccgtcac gtccgcggcg atcaccggcg tggcgttgcc gtgttgcggc
     2281 ggttgccacc ggggcggtcc gccaacgggc gtcgggaccg gcggcgacgc ccgcgcgacc
     2341 agttcgttcg actccgacag gctgtaggcc gtctcgccgg ccgcggactt ccacagaaac
     2401 gcctcgcccc cctcggccgc gacgagcttc ggccgcggac gcgaccgcgt gttgctgtat
     2461 tggcgcagcg tcagctcgtg ccccgtcgtg acaagctgcc ccgcatcgtc cacccgcgcc
     2521 agcgtctcga atccgtccga cgtcagccgg cagatctcca gccgatagcc ctcggcggcc
     2581 cggatccccc gttcgtccgg cggctcccgg ctcatcagga acagcgccgg cccgtcggcc
     2641 gtcaggcgtt caatcccccg cagaaaatat ccgggccgct ccgcgatcac gccgctcgtc
     2701 cgtgcgtcgt caacccgcac gtgccatcgg tcgccctgcg cgtggctcca gatgctgtag
     2761 gccatgtcga tccggccgtc agcattaaag tcgccaaccg acgtcggcag cgcctcatac
     2821 cgcgccctcc actcgtcggg ataattttcc tccaccaggc tgcgatacca cagcacccgc
     2881 ggccgctcgc ccagcttgac cacctccatg tgcggatacg actcgtcggt caggatcacc
     2941 agttcgtcgt ccggttcttc atccaggttc accgcttcga tgaacccgta gttgcgccgg
     3001 ccgctgccga atttcagatg ggcgatcgtc tcgcccgtcc gggcatccag cgcgctgatc
     3061 tcctgatgcc cctgcgcgat cacctccagc accccgtcgc cgttggcgtc ggccatatac
     3121 gtcttggggt aggcggccag gtggatctcg cgctgccaga gaaatccggc gtcgcgcgcc
     3181 ccctggctga agtccaccag cgcgatccag ccgccggcga catccccctt gtcatcctcg
     3241 cgcttcagat aaatcagaaa ctgctcgccg gcggcgtcgg gcagcaaccg ctccagccgc
     3301 cagtacgtga tgtgcgtcgc ctgcggatgc aagtcgatcc gcgacagcat ggccccgtcc
     3361 tccggcgaca cgaccaccaa ctgcggtggg tagcccgtca gcaggagttc cttccgcccg
     3421 tcgccgtcca cgtcctcggc ggccagcagg ctggtgtggc ggatcgtctc ggtctcccag
     3481 agcgtttcgc cctcggcggt ctgcagcgtg agccggcgac cgagcagaaa
//
LOCUS       DAOPCO010000370         3521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21443_length_3521_cov_5.930179, whole genome shotgun sequence.
ACCESSION   DAOPCO010000370 DAOPCO010000000
VERSION     DAOPCO010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21443_length_3521_cov_5.930179"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..1013
                     /locus_tag="PLS90_16455"
     CDS             342..1013
                     /locus_tag="PLS90_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPK04038.1"
                     /translation="MLDELRNQAGEDPSPSADNPKIPKAVIKRLSLYTRVLQKFSMSG
                     IEKISSKELGDALGLNSAQVRKDLAYFGQFGVPGLGYYVEELRTRIKSILGTDRTVRL
                     ALVGVGNLGQALLSYTTQFQRDGFRVVVAFDADKRKVGGERMGVPVFHVSDMGRHLKE
                     MAIDIVMLTVPAGVAQQVTDLAIQAGITAILNFVPERLLVPEHVKVHYVDLSIEIESL
                     SYYLK"
     gene            1288..2109
                     /locus_tag="PLS90_16460"
     CDS             1288..2109
                     /locus_tag="PLS90_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012900548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPK04039.1"
                     /translation="MSAPSAPRALILTGYGLNCEAETAVALARAGARVDRLHLNDLLA
                     RPARLADYQLLAIIGGFSFGDHLGAGRVLAIRLRHGLADQLADFVAAGGLVIGICNGF
                     QVLCKLGLLPAFTPFSRRSPQLVTLTHNDSGVFRDDWVHLRVNPASPCVFTRGLERLD
                     LPVRHGEGKFLALNDEVLARLHAGNQIVYQYADDAGAPTQSFPANPNGSVDAIAGICD
                     PTGRIFGTMPHPEAFILPEHHPDAPRLRALGLPLPEPMGMRIFANAVAALTPVPA"
     gene            2267..2980
                     /locus_tag="PLS90_16465"
     CDS             2267..2980
                     /locus_tag="PLS90_16465"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPK04040.1"
                     /translation="MESPANFPRPFNGDDDDMLPEYLDIPIFPLPNVTFFANTLLPLH
                     IFEPRYRTMVHNALCGDRLIGVTLLKEGWQNDYFGRPPICKTFGVGKIIDSEQLDDGR
                     FNIVLEGLYRVRLIEEFPTRPYRTGRVQVFQEMHIDDIRADISRLMKELQILTRRLSR
                     LLPASRELIQAAWAAHPHPLVVANHLASSLVIDPYDRQSILEQNDPIRRLNLILIQIR
                     NILLQLDARQIREEVIEED"
     gene            complement(3135..>3521)
                     /locus_tag="PLS90_16470"
     CDS             complement(3135..>3521)
                     /locus_tag="PLS90_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04041.1"
                     /translation="DEVPPPDQKELIHQIADKIREYDQVEMLSEVNPEMRQRYAREMH
                     LTPDKIERKHVLEHLQAELLEIEREMLRAADHERELGAPYNYKERQLVQMDRMLPTAQ
                     QMIDGGRATGPMEGEAKLPAEAGQPA"
BASE COUNT          569 a         1403 c          899 g          650 t
ORIGIN      
        1 cacccctcat ccctcatcct ctccgcctcg acccctcaat ccctaatccc tgacccccac
       61 atcatgttta aggctggaca cgccccgagt ttgtgaatta tgttacaaag cctgtgcagc
      121 acgcgccctc ccggcgtatg ctgaaggggt ggcggcgggg aggggcgccg cggccccgcg
      181 ggatttcccg ctcggttcgc attggcatgc cggtgccgct caccgcgcgc cgcggcctgc
      241 gaatgcgggc gaccccggcg acgtgtgccg ccccggcggg cgcgccggat ggggcggggg
      301 cgcctcacac atttcgaatc gccgtcacag ggtctgaccg gatgctcgac gagttgcgga
      361 accaggcggg ggaggatcct tccccgtccg ccgacaaccc caaaatcccc aaagccgtca
      421 tcaaacggct gtcgctctac acgcgcgtcc tgcaaaaatt cagcatgagc gggatcgaaa
      481 agatctccag caaggaattg ggcgacgccc tcggcctcaa ttccgcccag gtccgcaagg
      541 acctggccta cttcggccag ttcggcgtgc cgggcctggg ctattacgtc gaggaactcc
      601 gcacgcgcat caagtcgatc ctgggcaccg accgcaccgt ccgcctcgcc ctcgtcggcg
      661 tcggcaacct cggccaggcc ctcctcagtt acaccaccca gttccagcgc gacggcttcc
      721 gcgtggttgt cgccttcgat gccgacaaac gcaaggttgg cggcgaacgc atgggcgtcc
      781 ccgtctttca tgtcagcgac atgggccgcc acctcaagga aatggcgatc gacatcgtca
      841 tgctgaccgt tcccgccggc gtcgcccagc aagtcaccga tctggcgatc caggccggca
      901 tcaccgcgat cctcaatttc gtccccgaac gcctcctcgt ccccgaacac gtcaaggtcc
      961 actacgtcga tctctcaatc gaaatcgaaa gcctctccta ctacctcaaa taagaactcg
     1021 tccctcgtcc tcggctctcg aaaatctatc gggagcccgc gccagcgggc tttctactgg
     1081 cccctcgggg gcctgacatt agagccaggc ctcggcccgc tgaccgatcg ctttaaatct
     1141 tcttaaccgt ttcaacggtt tcctccacaa tccggacaag ccgatgttct cctcgacaaa
     1201 tcacttggcc ttccgcccgc ccgcgtgtca cgatggcaac agtccgcggc gttccgccgc
     1261 tcgaactcgc gaggtccctc gccccccatg tctgcgccat ccgccccccg cgccctgatc
     1321 ctcaccggtt acggcctcaa ctgcgaggcc gagaccgccg tcgctctcgc ccgcgccggc
     1381 gcccgcgtcg atcgcctcca cctcaacgac ctgctcgccc ggcccgcccg gctcgccgac
     1441 tatcaactcc tcgccatcat cggcgggttt tcattcggcg atcacctcgg cgccggccgc
     1501 gtccttgcca tccgcctgcg ccacggcctg gccgatcagc tcgccgactt cgtcgccgcc
     1561 ggcgggctcg tcatcggcat ttgcaacggc tttcaggttc tctgcaaact cggcctactc
     1621 cccgccttca cccccttctc gcgccgctcg ccgcagctgg tcacgctgac ccacaacgac
     1681 tcgggcgtct tccgcgacga ctgggtgcat ctgcgcgtca atcccgcttc tccctgcgtc
     1741 ttcactcgcg gtctcgagcg gctcgacctg ccggtgcgcc acggcgaggg caagttcctc
     1801 gcgctcaacg acgaagtcct cgcccggctc cacgccggca atcagatcgt ctatcaatac
     1861 gccgacgacg ccggcgcgcc gacccagtcc tttccggcca atcccaacgg ctcggtcgac
     1921 gccatcgccg gcatctgcga tcccaccggc cgcatcttcg gcacgatgcc gcaccccgaa
     1981 gccttcatcc tccccgagca ccatcccgat gccccgcgcc tgcgcgcgct cggcctcccc
     2041 ctgcccgaac cgatggggat gcgcatcttc gccaacgcgg tcgcggcgct cacgcccgtt
     2101 cccgcctgag cacccgaccg cctccgccgc gcccccatcc agcccggctt gtcaagcaat
     2161 tccgccgaaa aatcacgctt tttcgcccga tcccgccgcg gcgcgagatt ttctttgggc
     2221 ccttcccgcc ctccgtttat catggggcag agggaagatc gctccgatgg aatcgcccgc
     2281 aaacttcccc cgacccttca acggcgacga cgacgacatg ctccccgagt atctcgacat
     2341 cccgatcttc ccgctgccga acgtcacgtt cttcgccaat accctgctcc ccctgcacat
     2401 cttcgagccg cgctaccgca ccatggtcca caacgcgctg tgcggcgacc gcctgatcgg
     2461 cgtcacgctg ctcaaggaag gctggcagaa cgactacttc ggccgcccgc cgatctgcaa
     2521 aaccttcggc gtcggcaaga tcatcgacag tgaacaactc gacgatggcc gcttcaatat
     2581 cgtcctggag ggcctctacc gcgtccgcct gatcgaggag ttccccacca ggccctaccg
     2641 caccggccgc gtccaggtct ttcaggaaat gcacatcgac gacatccgcg ccgatatcag
     2701 ccgcctgatg aaagaactcc agatcctcac ccggcgcctc agccgcctgc tccccgcctc
     2761 gcgcgagctg atccaggccg cgtgggccgc gcacccccac ccgctcgtgg tcgccaacca
     2821 cctcgcttcc tcactggtca tcgaccccta cgatcgccag tccatcctcg agcagaacga
     2881 tccgatccgg cggctcaacc tgatcctgat ccagatccgc aacatcctgc ttcagctcga
     2941 cgccaggcag atccgcgagg aagtcatcga agaagactga ctccccttcg tccctccccc
     3001 tgaattcgtc cctcgtcctc gtaatcgccc caacggcccg ctcaaaaaag tcggctcccg
     3061 gctctcttct ctcgtcctcg gctctcgaaa tcaacccgtc cgccatacca tcccccccga
     3121 cacccccccg ccgcttacgc cggctgaccc gcttcggccg gcagtttcgc ttcgccttcc
     3181 atcggacccg tcgcgcgccc cccgtcgatc atctgctgcg ccgtcggcag catccggtcc
     3241 atctgcacca gttggcgctc tttgtaatta tacggcgcgc ccagttcccg ctcgtgatcc
     3301 gccgcgcgca gcatctcgcg ttcgatctcc agcaactcgg cctgcagatg ctccagcacg
     3361 tgcttccgct cgatcttgtc cggcgtcagg tgcatctcgc gcgcgtagcg ctggcgcatc
     3421 tccgggttca cttccgacag catttccacc tggtcatact cgcggatctt gtcggcgatc
     3481 tggtgaatca gctccttctg atccggcggc ggcacttcgt c
//
LOCUS       DAOPCO010000371         3498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21616_length_3498_cov_6.419983, whole genome shotgun sequence.
ACCESSION   DAOPCO010000371 DAOPCO010000000
VERSION     DAOPCO010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21616_length_3498_cov_6.419983"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..1022
                     /locus_tag="PLS90_16475"
                     /pseudo
     CDS             294..1022
                     /locus_tag="PLS90_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1551..2072
                     /locus_tag="PLS90_16480"
     CDS             1551..2072
                     /locus_tag="PLS90_16480"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HPK04042.1"
                     /translation="MQYHDGAAARPFSTRRIAAGGLVKIARRDGAVLRRAMLLGADGL
                     PRLLFHVDDEIIIAAEMLVEQPIDGGCQLGILVRDRFGNDVFGESRSSIQLGLPDTLQ
                     PGDLIRLRCQFRAALRQDLYFLTFGMTDLTWANTYFYGMDMVRLPLRPAGHEVFGIAN
                     LPHAFEAELIAGA"
     gene            2242..>3498
                     /locus_tag="PLS90_16485"
     CDS             2242..>3498
                     /locus_tag="PLS90_16485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPK04043.1"
                     /translation="MLNVDLVKDRARWDAVAEKYDTEINHVDRLLAAEIAAVLAPHLG
                     SNGHLLEAGSGSGHLSAVLAEHGYQTSLLDFSQVALEAGRRTFARYGLTGEFLEGNLF
                     DMQALGLARFDCVWNSGVLEHYQEEGTVAALREMAACSRNYVMALVPNGQSVFYRAFR
                     QFMHRHGEWEWGEENLHESLEPFFEQAGLEVLAETWVDGGYTEYFIRRMSQNEEFSRF
                     FWDLYDDNIIPPNQRYLLLTFGRIVTRQPGDFTATTRGQSTLAVMKRKLEAKNWMIAR
                     LAESLADQEKKIIQRERQLESQGGTREQLQVQIAELRERLAVKENTIQVLENEIQPKE
                     SRLVELMQQLAVREHEIQELTVQLHPLASELKGLRENLAMTQQALAEQRNHYEEQLAE
                     ARATIATLGQQRDQAQNELAIARHDLA"
BASE COUNT          598 a         1317 c         1030 g          553 t
ORIGIN      
        1 ccccgccgcc ccttctgacc cccacgccca acttcgtctc tcgtccccgg ctctccaaat
       61 ctcgccatgt cgctcctgcg tactacatcg ccgatccatt ccccgcggtc tctcaacgtg
      121 caagatcccc gtccccgcag ggggcgaatc cccgcggttc catggcgtgc aacccccaca
      181 accccaataa taccagttga agatcgccgc gctgcatcgg cacagtatcc cttgtgttgc
      241 cgaggagccc cagctctggt cccgcgcccc tcgcgccgga cgggtcgttt tccatgcagg
      301 cggcgagcgg ccgactcccg ctgatcgccc gcggcctctc caagtgctat cacatctacg
      361 accgcccaca ggaccggctc aaggcggccc tcctgccgcg cggcgcccgc ttttaccgcg
      421 agttctgggc actgcgcgac gtctccttcg aagtcgaacc gggcgaggcc gtcggcatca
      481 tcggccgcaa cggctcgggc aaaagcaccc tgctccagat cgtcgccggc acgctcctcc
      541 cctccgccgg cgaggtccac gtgaccggcc gcgtctccgc cctgctcgag ctgggcagcg
      601 gcttcaatcc ggaattcacc ggccgcgaga acgtcctgct caacggcagc atcctcggca
      661 tccgccgcga acggatggag gaaatcttcg acgagatcgt cgccttcgcc gacatcggcc
      721 ccttcgtcga tcagccggtc aaaaactact ccagcggcat gctcgtccgc ctggcgtttg
      781 ccgtcgccgt cgccgtccgc cccgacctgc tgatcgtcga tgaggcgctc gccgtcggcg
      841 acatcgcctt ccagcgcaag tgctatcgcc ggctcgccga aatgcgccag gacggcatga
      901 ccctcctctt cgtcacccat gaccccgggc tcatccgtca gctttgcggc aaggcggtcg
      961 tcctggagga ggggcgcatg ctcggctacg gccccgccgc cgaaatggcc gacctctatc
     1021 tggaactggt gctgggcaag gacgcccgcg tcaaccgccg cgtcgaagcc cggcacgcgc
     1081 ccgaacgcac ggccgccgtc tccacggccg acgacgagct gttcgcctcg gtccccagcg
     1141 aaatgcgctt cgagctgggc gaggaagccg agcgcgaggt cggcgtctac ggcgacgagg
     1201 aaggcgccgt cagcaacacg atggccccgt tcgagctcgc cgccggtcgc tatagcctgc
     1261 aggtcgattt ggcgcaccgc gccgaccgcg acgacgccct ctcgctgatg ctcgccgact
     1321 ggcagacgct caagaccgcc cacggcgcct atttccccgc cgccggcgcc tcgacccagc
     1381 tccgcaccca caccttcgag ttcgaagtcc gcgagccgct cgatcacgcc gaaatccgca
     1441 tcgtccgcac cggccgcttt cccgtcgatc tggccgagat ccgcctctac cgccgcgacg
     1501 acgaagaccc cgcccgcgtc gcgctgatcg gccgctggct cgttcaggag atgcagtatc
     1561 acgacggcgc ggccgcgcgc ccgttctcca cgcgccgcat tgcggccggc ggcctggtca
     1621 agatcgcccg gcgcgacggc gccgtcctgc gccgcgccat gctcctcggc gccgatgggt
     1681 tgccccggct gctgttccac gtcgacgatg aaatcatcat cgccgccgaa atgctcgtcg
     1741 aacagcccat cgacggcggc tgccagttgg gcatcctcgt gcgcgaccgg ttcggcaacg
     1801 atgtcttcgg cgaatcgcgc tccagcattc agctcggcct gcccgacacc ctccagcccg
     1861 gcgacctgat ccgcctgcgc tgtcagttcc gcgccgccct gcgccaggat ctctatttcc
     1921 tcaccttcgg catgaccgac ctgacctggg ccaacaccta cttctacggc atggatatgg
     1981 tccggctgcc gctgcggccg gccggccacg aggtcttcgg catcgccaac ctgccccatg
     2041 ccttcgaagc cgagctgatc gccggggcct gacggccgcg ggtccgccgg gccggagggc
     2101 ggcccgcccc gcgcctgaaa tgggcatgac agaaccccat gtcgcggtat aatgacgcgg
     2161 ggatcaatca aggtgaaacc ggatagggtc ggtcgccggc gggacgctcc cgcggccgcg
     2221 ggttccggtt cgaggataag catgctgaat gttgacctgg tgaaggaccg cgcgcggtgg
     2281 gacgcggtcg ccgaaaaata cgacaccgag atcaaccacg tcgatcgcct gctggcggcc
     2341 gaaatcgccg ccgtcctcgc cccgcatctc ggctccaacg gccacctgct cgaggccggc
     2401 tccggctccg gccatctgag cgccgtcctc gccgaacacg gctaccagac ctctctgctc
     2461 gacttcagcc aggtcgccct cgaagccggc cgccgcacct tcgcacgcta cgggctcacc
     2521 ggcgaattcc tcgagggcaa tctcttcgac atgcaggccc tcggcctggc gcgcttcgac
     2581 tgcgtctgga actccggcgt cctggagcac taccaggagg agggcaccgt cgccgccctc
     2641 cgcgaaatgg ccgcctgcag ccgcaactac gtgatggccc tcgtccccaa cggccagagt
     2701 gtcttctatc gcgcctttcg ccagttcatg caccggcacg gcgagtggga atggggcgag
     2761 gaaaacctcc acgagtcgct cgaaccattc ttcgagcagg ccggcctcga ggtcctcgcc
     2821 gaaacctggg tcgatggcgg ctacaccgaa tatttcatcc ggcgcatgtc gcaaaacgag
     2881 gagttctccc ggtttttctg ggatctctat gacgacaaca tcatcccccc caaccagcgc
     2941 tatctgctgc tgacgttcgg ccgcatcgtc acgcgccagc cgggcgattt caccgcgacg
     3001 acgcgcggcc agtccaccct cgccgtcatg aagcgcaagc tcgaggcgaa gaactggatg
     3061 atcgcccgcc tcgccgaatc gctggccgat caggaaaaga aaatcatcca gcgcgagcgc
     3121 caactcgaat cgcagggcgg aacgcgtgag caattgcaag ttcagatcgc cgagctgcgc
     3181 gaacgcctcg ccgtcaagga aaacacgatc caggtcctgg aaaacgaaat ccagccgaag
     3241 gaaagccggc tcgtcgaact gatgcagcag ctcgccgtgc gcgagcatga aatccaggaa
     3301 ctgaccgtcc agcttcatcc gctggcaagc gagctgaagg gcctgcgcga aaacctggcg
     3361 atgacgcagc aggccctcgc cgagcagcgc aatcactacg aggagcaact ggccgaggcg
     3421 cgcgcgacca tcgccacgct cggccagcag cgcgatcagg cgcagaacga actggccatc
     3481 gcccgccatg atctggcc
//
LOCUS       DAOPCO010000372         3487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21680_length_3487_cov_3.276224, whole genome shotgun sequence.
ACCESSION   DAOPCO010000372 DAOPCO010000000
VERSION     DAOPCO010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21680_length_3487_cov_3.276224"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..672)
                     /locus_tag="PLS90_16490"
     CDS             complement(310..672)
                     /locus_tag="PLS90_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04044.1"
                     /translation="MASTKTKPRPDLKRLRALAEEAAPHVERLTETLAVARHENAEVL
                     RLVQEYLASLPGGLHPHDIAGLDRSAYLDADALRPACFLLLKCMRRIDSLHQLAIRSS
                     LYPRQTLYIPNGHANGRH"
     gene            complement(674..2344)
                     /locus_tag="PLS90_16495"
     CDS             complement(674..2344)
                     /locus_tag="PLS90_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HPK04045.1"
                     /translation="MDRLQLKTATGAGRVERNGGVYGAGRITGVSVITRGEARGHALW
                     IDDVFLAQVATAGREAKHIKARFDHPSFLGDVLGTVVGVFRDFRKDGNRVRADLHLLA
                     TAKDAPHGNLGDYVMSLAAEAPDLFGASIEFSPHQAMQRQFVEAHTVAGRFRSPDADN
                     TNNFIHAQLDRLFACDLVDEPAANPAGAFAATTGGGATQFQSNRGAKMLTELHAERRI
                     DVAIGRQLQQLVAAARKSPSGVIELTAGSDEHGGYSDPYGGFLVPAGPLLDLRPSAFS
                     RAGEILSRCLAIETNAPEGAPSWPIIDETQRGDGYRWGGVSAKWTAETQTPGLSRFAV
                     AVGSLRFNTLAVLVAVSNEVLEAPGAFGTMVRRVLAAEASYKLEQSIMAGTGAGQPAG
                     VIGSPATIEVAAEDGQTTASALVWENVAKMRARLAPECVAGATWIVNHELLEFIADLD
                     APAGALDFSGPRPRILGLPVLESEAAAAVGERGDIVLADFSQYVVAHRPEAFAISAHV
                     RFEHHESVIRFTLRVDGSPLWKSPVKLANGSTTVSPFVTLAARPGEGE"
     gene            complement(2419..2661)
                     /locus_tag="PLS90_16500"
     CDS             complement(2419..2661)
                     /locus_tag="PLS90_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04046.1"
                     /translation="MRWREGDDIQTRFCGCEIPENLHRKMKIEAATRGCTMKQLLAEA
                     LALVYGGGVADDRARLPATSDGRNRMVPTGSERINL"
     gene            complement(3015..3335)
                     /locus_tag="PLS90_16505"
     CDS             complement(3015..3335)
                     /locus_tag="PLS90_16505"
                     /inference="COORDINATES: protein motif:HMM:NF016975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HPK04047.1"
                     /translation="MKIPTPPKNLERSGRRVWKTVLSEYELDGSAREVLAVACLALDR
                     AEGARELIAEHGILLVDRFGQQKANPAAAIERDARGQFLSAMRQLGLDLEPIFDGPGR
                     PAGR"
BASE COUNT          591 a         1174 c         1077 g          645 t
ORIGIN      
        1 tagtagcaac cgtggtagca accccctgcc ctcgcccgcc ggatcggaca ggacccgtca
       61 tttcggtagg gacgacagaa gaacgccccc ggacgcggat ggcggcccag gggcgaggat
      121 gtgatactcg gcgggttttg atggcggggg gtcgcggccg gtcgaatgcc ggtcaggggg
      181 ggaaactgcc ccgcccgccg catagggaac ggcaggcggg gcgattgccg gggcagagag
      241 taaggcgcgg ccctgcctcg gcggggaaga tctattcgcc ggcccgcgcg gcgcgccctc
      301 agtctccgat cagtggcgcc cgttcgcgtg gccgttcgga atgtagagcg tctggcgcgg
      361 gtataagctt gatcggatcg cgagctggtg aagggagtcg attcgtcgca tgcacttcag
      421 caacaagaag catgcgggtc gcaatgcatc ggcgtccaaa taggccgacc ggtccagccc
      481 cgcgatgtcg tgagggtgga gtccgccggg cagggacgcg agatactcct gcacgagccg
      541 caagacctcc gcgttttcgt gccgggcgac ggccagcgtc tcagtgagcc gctcgacatg
      601 cggcgcggct tcctccgcga gcgcccgcag ccgcttcaag tccggcctgg gttttgtctt
      661 ggtcgatgcc atcttattcc ccctcccccg ggcgagctgc gagcgtcacg aagggcgaga
      721 ccgtcgtgct gccattcgcc agcttcaccg gacttttcca gagcggcgaa ccgtcaacgc
      781 gcagcgtgaa ccgaataacc gattcgtgat gctcgaaccg cacgtgcgcg ctgatcgcga
      841 acgcttcggg ccgatgcgcg acgacatact gcgaaaaatc ggccaacacg atgtcgcccc
      901 gctccccgac ggccgcggcc gcttccgatt cgaggacagg cagccccagg atgcgcggcc
      961 gcggtcccga gaaatcgagc gcgcccgccg gcgcgtccag atccgcgatg aattccagca
     1021 gttcgtgatt gacgatccag gttgcgccgg ccacgcattc cggcgcaagc cgcgcgcgca
     1081 tcttggcgac gttctcccag accagcgccg acgcggtggt ttgtccatct tcggcggcga
     1141 cttcgatggt tgccggcgag ccgatgacgc cggcgggttg ccccgcgccc gtgccggcca
     1201 tgatgctctg ctccagctta tagctcgctt cggccgccag gacgcgccgc accatggtcc
     1261 cgaaggcgcc gggcgcctca agcacctcgt tcgacacggc caccagaacg gcgagcgtgt
     1321 tgaaacgcag cgagccgacg gccacggcga accgcgacag gccgggggtc tgagtctcgg
     1381 cggtccactt cgccgagacg ccgccccacc ggtagccgtc gccccgctgg gtttcgtcga
     1441 tgatcggcca actcggcgcg ccttcgggtg cattcgtttc gatggccagg catcgactca
     1501 aaatctcgcc cgcgcggctg aatgcgctcg ggcgcagatc gagcaacggc cccgccggca
     1561 ccaaaaaccc gccatacggg tcgctgtacc cgccgtgctc gtcgctcccc gccgtcagtt
     1621 cgataacgcc gctcggactc ttgcgggcag ccgcgacaag ctgctgaagt tgccggccga
     1681 tggcgacgtc gattcgccgt tcggcatgta attccgttag cattttcgct cctcgattcg
     1741 attgaaattg tgtcgcgccg ccgccggttg tcgccgcaaa cgcgccggcc ggattcgccg
     1801 ccggctcatc gaccaggtcg caagcaaaaa gccggtccag ttgcgcgtga atgaagttgt
     1861 tcgtgttgtc tgcgtccgga ctcctaaaac gcccggccac cgtatgcgcc tcgacaaatt
     1921 gccgctgcat ggcttgatgc ggcgagaatt caatgcttgc gccgaacaag tccggcgcct
     1981 cggcggccag actcattaca taatcgccca ggttgccgtg tggtgcgtcc ttcgccgtcg
     2041 cgagtagatg caaatcggca cgcaccctgt taccgtcttt tcggaaatct cgaaaaacgc
     2101 cgacgacggt tccgagaacg tcgccaagaa acgatggatg atcgaaccgg gccttgatgt
     2161 gctttgcctc gcggccggcc gttgccacct gcgcaagaaa aacatcgtcg atccacagcg
     2221 catgcccccg cgcctcgccc cgcgtgatca cgctcacgcc cgtaatccgg cccgcgccgt
     2281 aaacgccgcc gttgcgctcg acgcgcccgg cccccgtcgc agttttaagc tgaagtcgat
     2341 ccatgaaact ctcctttcct aatgccaatt aggcaatggc gaggggccgg aaagatagca
     2401 ttaagtttat tatcccgatt ataagtttat gcgttcactc ccggtcggga ccatgcggtt
     2461 gcgcccgtcg cttgtggcgg gcagtcgcgc ccggtcatcg gcaacgccgc cgccgtagac
     2521 cagcgccagc gcttcggcca gcagttgctt catcgtgcaa ccgcgcgttg cggcttcgat
     2581 tttcattttg cgatgcaagt tttctggaat ttcgcacccg caaaatcggg tctggatgtc
     2641 gtcaccctca cgccaacgca tggttctggc cccctctctc aattggcatt aacaattcgg
     2701 ggtgcgcccg gagatattcc cgttgatccg accacgtcgg cgacttgaag ccgtcgcccg
     2761 gccccggcgc cgccgacggg atcggttgcg gcagtcgcga gagcggccag cgccaccacg
     2821 gagcgggacg ggtgtagtgg cgcgccgggt ccgccagcca atcggcgaga atttgccgcc
     2881 ggttcgccgt ccagacgtcc cgatcagcgt cagacggcgc gaattgaata aaccacagtt
     2941 cgccgggcga taggtcgcgc ggccgtgcgt cccgtcgttc cggtttccgc cgtcgtcgat
     3001 tcgtagccat cccatcacct ccccgccggt cggcccggcc cgtcaaaaat gggttcaagg
     3061 tccaaaccga gttgtcgcat cgctgataaa aactgacctc tcgcgtcccg ttcgattgcg
     3121 gccgcgggat tcgccttctg ttgcccgaag cggtccacca gcaggatgcc gtgctcggca
     3181 atcagttccc tcgccccttc ggcacggtcc agcgccaggc aggcaacggc gagcacctcg
     3241 cgtgcgctcc catcaagttc gtattcagac aaaacagttt tccagactcg ccggccagac
     3301 cgttccaggt ttttgggtgg ggttgggatt ttcatgctgt cgaatccttg ccagattttt
     3361 tataagggga ccttaatttt tcatgcgcgc ggggccgggt gcggtcacta cgccgcgact
     3421 tgtagagttt tcaccccgcc ccccccgttg gcccccacgg cccccgccca gggtgatgcg
     3481 cccatcg
//
LOCUS       DAOPCO010000373         3474 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_21758_length_3474_cov_5.776250, whole genome shotgun sequence.
ACCESSION   DAOPCO010000373 DAOPCO010000000
VERSION     DAOPCO010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21758_length_3474_cov_5.776250"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1132
                     /locus_tag="PLS90_16510"
     CDS             119..1132
                     /locus_tag="PLS90_16510"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK04048.1"
                     /translation="MQAAVTGAGGFIGSHLTELLLREGWRVRALVHYNALDRHGHLDE
                     VARRGREEGAAWWHAGRLEVVAGDVGDARALREWVRGHGVVFHLAALIGIPYSYRAPQ
                     SYVGTNVTGTLHLLEACREEKIERLLHTSTSEVYGSAVRTPIDEAHPLQAQSPYAATK
                     IAADKLVESYARSFDLPALVVRPFNTYGPRQSARAVIPTVLSQVLSGECGEVEIGSPD
                     PVRDWVYAEDTARAFLALARTELPTEPAAVFNVATGRGWTIGEMVERVQAVTGVKKPL
                     RTAEGRRRPAASEVDRLIGDAKRLRQLTGWEPEISFETGLERTANWISRHLELYRSGA
                     FTV"
     gene            1132..1848
                     /locus_tag="PLS90_16515"
     CDS             1132..1848
                     /locus_tag="PLS90_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK04049.1"
                     /translation="MTDPTPAAPPGRAVVLAGGQGTRLRPYTTVLPKPLLPVGDRPIL
                     EHVLLRLAEAGFRRVTLSVGHLAELIQVFFGDGARFGMQLDYAIEDQPLGTIGPLARI
                     AGLGENFLVMNGDVLCDLPIGALWERHGASGAALTIATFRREVKIDFGVLRYDGQCRV
                     RAFEEKPTLPYDVSMGIYVLNRRCLDFVPAAGAFGFDQLVLALLAAGEKVEAFPHEGN
                     WLDLGRPEDYERATREWGEG"
     gene            complement(1867..3360)
                     /locus_tag="PLS90_16520"
     CDS             complement(1867..3360)
                     /locus_tag="PLS90_16520"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HPK04050.1"
                     /translation="MTPAPLIHFQSVSKRFPGVQALHCVELPIAAGSCHALVGENGAG
                     KSTLGKILAGLHTPDEGRLLIDGRPCQFHSPHDAARAGVAMVHQELLFCENLCVAENL
                     SLGRLPARATFLRRRRMEQLAAQWLEPIDARIDPRRRVGELPVSQQQLVQIAAAIGRG
                     ARILVFDEPTSSLAHAESERLFDLIARLRRRGVTMIYVSHRMDEIFRLCDTISVLRDG
                     RHVATRPAAEYDRDSLVHDMIGRPVSDYFPGHLAAQPGQELLRVEGLSSPGRFHDISF
                     SLRRGEVLGLAGLVGAGRTELAEALFGLDPHAVGRVWLKGRPLALGRPRRALERGLGL
                     VPEDRKRHGLVLSLRARENITLPILPRLSRLTWVRRRAERRLAREFFDRLSVRAPSLE
                     TPARSLSGGNQQKLVIARWLAARCDVLIVDEPTRGVDVGAKAEIHGLIDRVARDGNAV
                     LLISSELPEVLNLSTRILVLREGQLAGELSRDAADQAALLRLMARLD"
BASE COUNT          510 a         1007 c         1460 g          497 t
ORIGIN      
        1 gggggggcat tggggttgcc ttcccggggc gggatgctac cttacggaca agacacgtgt
       61 gcgcacgaac atggcccgac gagcttgggc ttgttgaccg ccggggagcg attgatcgat
      121 gcaagccgcc gtgaccggcg ccgggggatt tatcggcagc cacctgaccg agctgttgct
      181 gcgcgagggg tggcgggtgc gcgccctggt gcactataac gcgctggacc ggcacgggca
      241 cctggacgaa gtggcgcggc gcggccgcga ggagggggcc gcgtggtggc acgccgggcg
      301 gctggaggtg gtggcgggcg acgtcggcga cgcgcgggcg ttgcgcgagt gggtgcgggg
      361 gcacggtgtg gtgttccacc tggcggcgct gatcgggatc ccgtacagct atcgcgcgcc
      421 gcagagttac gtgggcacaa acgtgacggg gacgctccat ctgctcgagg cgtgccgcga
      481 ggagaagatc gagcggctgc tgcacacatc gacgagcgag gtctacggga gcgcggtgcg
      541 gacgccgatc gacgaggcgc acccgctgca ggcgcagtcg ccgtatgcgg cgacgaagat
      601 cgccgccgac aaactggtcg agagctacgc gcggtcgttc gatctgccgg cccttgtggt
      661 gcggccgttc aacacgtacg ggccgcggca gtcggcgcgg gcggtgattc cgaccgtcct
      721 gagccaggtg ctgagcggcg aatgcgggga agtggagatt ggctcgcccg atccggtgcg
      781 cgactgggtg tatgccgagg atacggcgcg ggcgtttctg gcgctggcgc ggaccgagct
      841 gccgacggaa ccggcggcgg tgttcaatgt tgcgacgggc cggggctgga cgatcgggga
      901 gatggtcgag cgggtccagg cggtgaccgg cgtgaagaag ccgctgcgga cggcggaggg
      961 ccggcggcgg ccggcggcaa gcgaggtgga tcggctgatc ggcgatgcga agcggctcag
     1021 acaactgacg ggatgggagc cggaaatcag ttttgaaacc gggctggagc gcacggcgaa
     1081 ctggatttcg cgccacctgg aactttaccg aagcggagca ttcaccgtct gatgaccgac
     1141 ccgacacccg ctgcgccgcc gggacgcgcc gtcgtcttgg cggggggcca gggcacgcgc
     1201 ctgcggccct atacgaccgt gctgcccaag ccgctgctgc cggtgggcga ccggccgatt
     1261 ctggagcacg tgctgctgcg gctggccgag gccggtttcc gccgggtgac gctgtcggtg
     1321 gggcatctgg ccgagctgat tcaggttttt tttggcgacg gcgcgcggtt cgggatgcaa
     1381 ctggactacg cgatcgagga ccaaccgctg gggacgatcg gcccgctggc gcggatcgcg
     1441 gggctggggg aaaacttcct ggtgatgaac ggcgacgtgc tgtgcgacct gccgatcggg
     1501 gcgctgtggg agcgccatgg ggcgagcggg gcggcgctga cgatcgcgac gttccgccgc
     1561 gaggtgaaaa tcgatttcgg cgtgctgcgc tacgacgggc aatgccgcgt gcgcgccttc
     1621 gaggaaaaac cgacgctccc ctacgacgtg tcgatgggaa tctatgtgct aaaccggcgc
     1681 tgcctggatt tcgtgccggc ggccggcgcg ttcggcttcg atcagctcgt gctggcgctg
     1741 ctggcggcgg gcgagaaggt ggaagcgttt ccgcacgaag ggaactggct ggatctgggg
     1801 cggccggaag actacgagcg cgccacccgg gagtgggggg aaggttaggg cgcgggccga
     1861 cgcgggtcaa tccaggcggg ccatcaggcg caggagcgcg gcctgatcag cggcgtcgcg
     1921 gctcagttcg ccggcgagct ggccctcgcg caggacgagg atccgcgtgc tgaggttgag
     1981 gacttcgggc agttcgctgg agatgaggag gacggcgttg ccgtcgcggg cgacgcggtc
     2041 gatgaggccg tggatttcgg ccttggcgcc gacgtcgacg ccgcgggttg gttcgtcgac
     2101 gatcagcaca tcgcagcggg cggcgagcca gcgggcgatg acgagcttct gctgattgcc
     2161 gcccgagagg gagcgcgcgg gcgtttcgag cgacggggcg cggacgctga ggcggtcgaa
     2221 gaattcgcgc gcgaggcggc gttcggcgcg ccggcggacc cacgtcaggc ggctgaggcg
     2281 gggcaggatc ggcagcgtga tgttttcgcg ggcgcgcagc gagaggacga ggccgtggcg
     2341 cttgcggtcc tcggggacga ggccgaggcc gcgctcgagc gcgcggcgcg gccggccgag
     2401 cgcgagcggg cgccccttca gccagacgcg gccgacggcg tgcggatcga gcccgaaaag
     2461 ggcctcggcc agctcggtgc ggccggcgcc gacgaggccg gccaggccga ggacctcgcc
     2521 gcgccggagc gagaaggaga tgtcgtggaa gcggccgggg ctgctcaggc cctcgacgcg
     2581 cagcagttcc tggcccggct gggccgccag gtggccggga aagtaatcgc tgacgggccg
     2641 gccgatcata tcgtgaacga gcgagtcgcg gtcgtactcc gcggcggggc gggtggcgac
     2701 gtggcggccg tcgcgcagaa ccgagatcgt gtcgcagagc cggaagattt cgtccatccg
     2761 gtgcgagacg tagatcatcg tgacgccgcg tcggcgcagg cgcgcgatca ggtcgaagag
     2821 gcgctcgctt tcggcgtggg cgaggctgct ggtcggctcg tcgaagacga ggatccgcgc
     2881 gccgcggccg atggcggcgg cgatctggac gagttgctgc tggctgacgg ggagttcgcc
     2941 gacgcgccgg cgcgggtcga tgcgcgcgtc gatcggttcg agccattggg cggcgagctg
     3001 ctccatgcgg cggcgccgga ggaaggtcgc ccgggcgggc aggcggccga gcgagaggtt
     3061 ttcggccacg cacaggtttt cgcagaagag cagttcctgg tggaccatgg cgacgccggc
     3121 gcgggcggca tcgtgggggg agtggaactg gcacggccgg ccgtcgatca ggagccggcc
     3181 ttcatcgggg gtgtggagcc cggccaggat cttccccagc gtgctcttgc cggcgccgtt
     3241 ctcgccgacg agggcgtggc acgagccggc ggcgatcggc agctcgacgc agtggagcgc
     3301 ctgcacgccg ggaaaccgct tgctgacgga ttggaagtgg atcagaggcg cgggcgtcat
     3361 cggggcatgg tggcgaagcg aggaggggaa tgcaaggcgg gagggagctg gggggtgcgg
     3421 ggcggcaagg acgccggcgg tcccaggcgg tggggatggc ggagggtggg gggg
//
LOCUS       DAOPCO010000374         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22196_length_3418_cov_5.162058, whole genome shotgun sequence.
ACCESSION   DAOPCO010000374 DAOPCO010000000
VERSION     DAOPCO010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22196_length_3418_cov_5.162058"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PLS90_16525"
     CDS             <1..314
                     /locus_tag="PLS90_16525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPK04051.1"
                     /translation="LLGADWLNCAECQGPVILCGDFNALPGSQVCRLLRRRLRDSQLE
                     LDRHRPHKTYSTRYPVGRIDHIFVSPHFEVVKVEVARNNLACLASDHLPLIIEVRLKR
                     D"
     gene            882..2084
                     /locus_tag="PLS90_16530"
     CDS             882..2084
                     /locus_tag="PLS90_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPK04052.1"
                     /translation="MAKMIDAVAAGHICLDITPVFASGRVERVGDLLKPGKLVNTEPA
                     RISLGGPVANTGLALKRYGLRLAAAAGIGEDKFGHIARELLEEAGIGGAIRPVGGACT
                     SYTVVLAPPGIDRIFLHCPGRNDEFQLDDIDFATVAAARLFHFGYPPLMRGVYENDGR
                     LLTAIFERAKACDATTSLDLSLPDPDSASGKVDWAGILARTLPAVDIFMPSIEELYFM
                     LERERYLELRRRHPDADLVELIPVEDYLRLAERAIALGCGLCVIKTARRGYYLRGGSP
                     ERLARFGGGAGAAAERWAHRQLWAHAYHVRAIASATGSGDSSIAGFLAAFLRELPPER
                     CMCLANASGHHNLLALDALSGLPDWDGLVAFVDRMPRAEDPLLEGSEFRFDEQLQVYV
                     GPRDGRAA"
     gene            2096..3064
                     /locus_tag="PLS90_16535"
     CDS             2096..3064
                     /locus_tag="PLS90_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK04053.1"
                     /translation="MKIACVIPAHNEERFIGGVLRAIPPRVERVFVVDDASRDGTAAA
                     VAAVGDPRVTLLRHEVNRGLGAAMATGYRAALATDAEIVVKLDGDGQMDPAEIERLVL
                     PIEEGLADYCKGVRFRGRDVIRRMPPVRLAGNLGLSFLTKIATGYWDLLDPTNGFTAI
                     GRPALRQLNLDRVARGFFFETDMLFHLYLAGAVAMDVAIPARYGEETSDLSPLRALIT
                     FPWWLLRVFVRRIVWRYFIIDFSAVSAFLIVGGALFGWGLVFGLWKWIQMAVFVGAAT
                     PTGTIMLAAVPLILGFQLLLQAVVLDIQNVPRIPLQRKQARHDERR"
     gene            3171..>3418
                     /locus_tag="PLS90_16540"
     CDS             3171..>3418
                     /locus_tag="PLS90_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04054.1"
                     /translation="MGLRQQLAEDWRWYRTVEGGRSFFRPLIEYGFWIVAVHRLGAWS
                     RALRVPLVGFALRQVYRVARFFVFAVTGVDIRPGARIGR"
BASE COUNT          493 a         1084 c         1284 g          557 t
ORIGIN      
        1 cgctgctcgg ggcggactgg ctcaactgcg ccgagtgcca ggggccggtg atcctctgcg
       61 gcgacttcaa cgcgctgccc ggctcgcagg tctgccgcct gctgcgccgc cgcctgcgcg
      121 acagccagct cgaactcgac cgccaccgcc cgcacaagac ctactcgacg cgttaccccg
      181 tcggccgcat cgaccacatc ttcgtcagcc cgcacttcga ggtcgtcaag gtggaagtcg
      241 cccgcaacaa cctcgcctgc ctcgcctcgg accacctccc gctgatcatc gaagtccgcc
      301 tcaagcgcga ttgagcgggc agggggcggc gatttccccg ctggggaagt attatacaag
      361 gggggaggga gagaaatgaa ttccctcagg aacccatagg ggaatcaggg ggaacataat
      421 tttggggggg agggaggaat ggggaagata tggatcacga aaggcacgaa agggcacgaa
      481 agaggtcgag agactcgcaa aagcgaaagg ctcaaagaag ggcatgatgg atcggtgtcg
      541 ggatcggaat cgagtcggcg cccggccgca tcgtgacgca cagacgcggc gtgcgtcttg
      601 ttttgggagg gcggcggcgt ctctgcgctt gcaggggaag agcaggcggg gcaaactcgg
      661 gggccggtcg gtgcggtgcg gaccgtcgcc cgcggcgcga cgaggccacg cggccggcgt
      721 cccgcgccgc gacgaacccg tgtcgccgcc aagcttagct tgcgcggcgc gacccagcgg
      781 cgctgcaacc gacgcgcgcc gaggcgaacc gattcgcgcc gcccatgccc ccgcgccgcg
      841 gctgacccgc gcggccgggc cacttcattc aggatgccga catggccaaa atgatcgatg
      901 ccgttgccgc cgggcacatc tgcctggaca tcacgccggt gttcgccagc gggcgcgtcg
      961 agcgcgtcgg cgacctgctc aagccgggca agctggtcaa caccgagccg gcgcggatct
     1021 cgctgggggg cccggtggcc aacacggggc tggcgctcaa gcgttacggg ctgcggctgg
     1081 ccgccgcggc ggggatcggc gaggacaagt tcgggcatat cgcgcgcgag ctgctggaag
     1141 aagcggggat cggcggggcg atccggcccg tcggcggcgc atgcacatcg tatacggtcg
     1201 tgctggcgcc gcccggcatc gaccggatct tcctgcactg cccgggccgg aacgacgagt
     1261 tccaactgga cgacatcgat ttcgcgacgg tggcggcggc gcggctgttc cacttcggct
     1321 acccgccgct gatgcgcggc gtgtatgaga acgacggccg gctgctgacc gcgatttttg
     1381 agcgtgccaa ggcgtgcgac gcgacgacgt cgctcgacct gtcgctgccc gacccggact
     1441 cggcctcggg caaggtcgac tgggcgggga ttctggcgcg gacgctgccg gcggtggaca
     1501 tcttcatgcc gagcatcgaa gagctgtact tcatgctgga gcgcgagcgg tatctggagc
     1561 tgcgccggcg ccatcccgat gccgacctgg tcgagctgat accggtggag gattatctgc
     1621 ggctggcgga gcgggcgatc gcgctgggct gcgggctgtg cgtgatcaag acggcgcggc
     1681 gcggctacta tctgcgcggg ggttcgcccg agcggctggc gcggttcggc ggaggggcgg
     1741 gcgcggccgc cgaacgctgg gcgcaccgcc agctctgggc gcacgcctac catgtgcggg
     1801 cgatcgcctc ggcgacgggg agcggggatt cgtcgatcgc cgggttcctc gccgcgtttc
     1861 tgcgcgagct gccgcccgag cgctgcatgt gcctggccaa cgcttcgggc catcataatc
     1921 tgctcgcgct cgatgcgctc tcggggctgc ccgactggga cggtctggtc gcgttcgtcg
     1981 accggatgcc gcgggcggag gacccgctgc tggagggatc ggagttccgg ttcgacgagc
     2041 aacttcaggt ttatgtcggc ccgcgcgacg ggcgcgcggc ctgagcccgg cgccgatgaa
     2101 gatcgcctgc gtcatccccg cgcataacga ggagcggttc atcggcgggg tgctgcgcgc
     2161 gatcccgccg cgggtggagc gggtgttcgt ggtggacgac gcgagccgcg acgggacggc
     2221 tgcagcggtc gcggccgtcg gcgatccgcg cgtgacgctg ctgcgccacg aggtcaaccg
     2281 cgggctgggc gccgcgatgg cgaccggcta ccgcgcggcg ctggcgaccg acgccgagat
     2341 cgtcgtcaag ctcgacggcg acggccagat ggacccggcg gagatcgagc ggctggtgct
     2401 gccgatcgag gaggggctgg ccgactactg caagggggtg cgcttccgcg gcagagacgt
     2461 gatccgacgg atgccgccgg tccggctggc gggcaacctg gggttgtcgt ttctgacgaa
     2521 aatcgccacc ggctactggg acctgctcga cccgaccaac gggttcaccg cgatcggccg
     2581 gccggcgctg cgccagctca atctggaccg ggtcgcgcgc ggtttcttct tcgagaccga
     2641 catgctgttt catctgtacc tggccggcgc cgtggcgatg gacgtggcga ttccggcgcg
     2701 ctacggcgag gagacgagcg atctgagccc gctgcgcgcg ttgattacgt ttccgtggtg
     2761 gctgctgcgg gttttcgtcc ggcgcatcgt gtggcgctat ttcatcatcg atttttccgc
     2821 ggtctcggcg ttcctgatcg tcggcggggc actgttcggc tgggggctcg tcttcggcct
     2881 gtggaagtgg atccagatgg cggtcttcgt gggcgcggcg acgccgaccg ggacgatcat
     2941 gctcgccgcc gtgccgctga tcctggggtt tcagctgctg ctgcaggcgg ttgtgctgga
     3001 catccagaac gtgccgcgga tcccgctgca gaggaaacag gcgcgccacg acgagcggcg
     3061 ctgaacgccg gcagccggcc gcgcgggggc tccggcccgc caagtagggg aagatttttg
     3121 ggggtgttcg ccttaggata agccgcgaac gggaatggcg gtcggcgcaa atgggtctca
     3181 ggcaacagct cgcggaggac tggcggtggt accgcacggt ggaggggggg cgctcgtttt
     3241 tccgcccgct gatcgagtat ggcttttgga tcgtcgcggt ccaccggctg ggagcgtggt
     3301 cgcgcgcgct gcgcgttccg ctcgtcggct tcgcgctgcg ccaggtctac cgtgtcgcgc
     3361 gcttctttgt gttcgccgtc acgggggtcg acatccgtcc cggggcgcgg ataggccg
//
LOCUS       DAOPCO010000375         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22199_length_3418_cov_3.915552, whole genome shotgun sequence.
ACCESSION   DAOPCO010000375 DAOPCO010000000
VERSION     DAOPCO010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22199_length_3418_cov_3.915552"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLS90_16545"
     CDS             complement(<1..1397)
                     /locus_tag="PLS90_16545"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPK04055.1"
                     /translation="MHRPLRLIASLALVLVAALAAGQYPPPGPTPGELQSPLGRAAAA
                     QRLQAAGQADRKQARAKAAARGDLIRGQTPAGQIFELVGEAAGIPLYYITDNQQAAIS
                     SGAALIRQMPPDQLDGAGLIAGIWDEGRCRLTHFEFTGRARSLDGAATSRHATHVAGT
                     LIGVGLMDTSRGMAPAAALDSYDWNLDLAEMTERAAAAPVPGARILISNHSYGTIAGW
                     ATGAYSGQTAPHWFGHWGLREDEKFGQYSNSASSWDQLCHSAPWYLPVKSAGNDRSNG
                     APRDGALFFYFDDAWTSATYNPAIHPLADFQHGGYDTLPTISVAKNILTVGAVSDAVS
                     GGQRHPPAAQMTSFSGWGPTDDGRIKPDLVANGVDLLSASSENDAAYASLTGTSMAAP
                     NAAGSALLLQQLHMKLNQGRALRSATLKALLIHTADDLGRPGPDYAFGWGLLNVAAAA
                     EILRRQAEAPGAPLVT"
     gene            1560..2348
                     /locus_tag="PLS90_16550"
     CDS             1560..2348
                     /locus_tag="PLS90_16550"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014996213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK04056.1"
                     /translation="MTESTASAELVRAEGLGLTFPSGVRALAEVELAVAAGEFVAIVG
                     PSGCGKSTFLRVLAGLIEPTAGRVTVDGMAPDQARRRSHELAFVFQHPTLLPWRRVID
                     NLRLPLELGRRKGQRRATAEELRAALRRVGLSDFARAWPHQLSGGMQMRLSLARALIT
                     DPRLLLMDEPFGALDDLTRQQLNEELLRLWQDRRWTGLFVTHNVAEAVYLSQRVLVMS
                     PRPGRFILDVPVPFDYPRGAELRGEAEFARLTRQISERLMERAA"
     gene            2345..3127
                     /locus_tag="PLS90_16555"
     CDS             2345..3127
                     /locus_tag="PLS90_16555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK04057.1"
                     /translation="MIQRALRFLADRLLPPVVVFVVFVLVWHELVVWLEVPPLLVPTP
                     ARVWGAALEHREALLRAMRLTGAAALCGLALAIVVGSLTAFVFSQSVVIQRGFYPYAI
                     FLQTVPVVAIAPLIILWFGQGFQSVVIVAFILSLFPIITNGTTGLTAIDRGHRELFAI
                     HNATRWQVLVKLRIPNAVPYLVTGARISSGLSVIGAIVGEFFAGFSDRNRGLGYLIQL
                     SHGQLRTAYLFAAILASTLLGLAIFALVTWIGNAIVARWREA"
BASE COUNT          451 a         1042 c         1338 g          587 t
ORIGIN      
        1 gtgacgagcg gcgcgccggg ggcttcggcc tggcggcgca ggatctcggc ggcggccgcc
       61 acgttgagca aaccccagcc gaaggcgtaa tccgggccgg gacggccgag atcgtcggcg
      121 gtgtggatca gcagcgcctt gagcgtcgcg gaccgaagcg cgcgcccctg gttcagtttc
      181 atgtgaagct gctggagcag cagagccgag ccggcggcgt tgggggcggc catgctcgtg
      241 ccggtgagcg aggcgtaggc cgcgtcgttt tcggacgagg cggaaagcag gtcgacgccg
      301 ttggcgacaa ggtccggttt gatccgtcca tcatcggtcg gaccccagcc gctgaaggag
      361 gtcatctgcg cggcgggcgg atggcgctgg ccgccgctga cggcatcgga aaccgcgccg
      421 acggtcagga tgttcttggc gacggagatc gtgggcagcg tatcgtagcc gccgtgctgg
      481 aagtcggcca gcggatggat ggcggggttg taggtcgcgc tggtccaggc gtcgtcgaag
      541 tagaagaaca gggcgccgtc gcgcggggcg ccgttgctgc ggtcgttgcc ggccgacttg
      601 accggcagat accagggcgc ggaatggcag agctggtccc agctgctggc cgagtttgaa
      661 tactggccga acttttcgtc ctcgcgcaga ccccagtggc cgaaccagtg gggcgcggtt
      721 tgcccggagt aggccccggt ggcccagccg gcgatggtgc cgtaggagtg gttggagatc
      781 aggatgcggg ccccggggac cggggcggcg gcggcccgtt cggtcatctc ggccagatcg
      841 agattccagt cataggaatc gagggcggcg gccggcgcca tgccgcgcga cgtatccatc
      901 aggccgacgc cgatcagcgt gccggcgacg tgggtggcat ggcggctggt cgcggcgcca
      961 tcgagcgacc gggcgcggcc ggtgaactcg aagtgggtca ggcggcagcg cccttcatcc
     1021 cagatgccgg cgatgaggcc cgcgccgtcg agctggtcgg ggggcatctg gcggatcagc
     1081 gccgcgccgc tggagatggc ggcctgctgg ttgtcggtga tgtagtagag tgggatgccg
     1141 gcggcttcac cgacgagttc gaagatctgg ccggccgggg tctggccgcg gatgagatcg
     1201 ccgcgcgccg ccgccttggc gcgcgcctgt ttccggtcgg cctggccggc cgcctgcaac
     1261 cgttgggcgg ccgccgcgcg ccccagcggg ctttgcagct cgccgggcgt cggacccggc
     1321 ggggggtact ggccggcggc cagggcggcc accagcacca gcgcaaggct ggcgatcagg
     1381 cgcaacggac ggtgcatggc tcattttacg gggagggccg ccgactcgca aggagcacgg
     1441 ttgcgggcgt tgccgggggc gtttccgcgc gcgcgggatg gtcggcgcgt tgacgagccg
     1501 cccccgcagt cgatatgatg cggccctccg cgagaatgac ggcgaaagga acccgcagcg
     1561 tgacggaatc gacggcatcg gcagagctcg tgcgggccga agggttgggc ttgacgttcc
     1621 cctccggggt gcgggcgctg gcggaggtcg agctggccgt cgcggcgggg gaattcgtcg
     1681 cgatcgtcgg gccgtcgggc tgcggcaaat caaccttcct gcgggttctg gcggggctga
     1741 tcgaaccgac cgcggggcgg gtgaccgtcg atgggatggc gccggatcag gcccggcgac
     1801 gatcgcatga actggcgttc gtgtttcagc acccgacgct gctgccgtgg cggcgggtga
     1861 tcgacaacct gcggttgccg cttgaactgg ggcggcgcaa ggggcagcgc cgcgcgacgg
     1921 cggaggaatt gcgggcggcg ctgcggcggg tcgggctgag cgacttcgcg cgcgcctggc
     1981 cgcaccagct ctccggcggg atgcagatgc ggctgtcgct ggcgcgggcg ctgatcaccg
     2041 atccgcggct gctgctgatg gatgagccgt tcggcgcgct cgacgacctg acgcgccagc
     2101 agctcaacga ggaactcctg cggctgtggc aggaccggcg ctggacgggg ctgttcgtca
     2161 cgcacaacgt ggccgaggcg gtctacctga gtcagcgggt gctcgtgatg agtccccgcc
     2221 cggggcggtt catcctggat gtgccggtgc cgttcgacta cccgcgcggc gcggaattgc
     2281 gcggcgaagc cgagtttgcc cggctgacgc gccagatcag cgaacgcctg atggagcggg
     2341 cggcatgatc cagcgcgcgc tccggtttct ggctgatcgg ctgctgccgc ccgtcgtcgt
     2401 cttcgtggtg ttcgtcctcg tctggcatga gctggttgtc tggttggagg tgccgccgct
     2461 gctggtgccg acgccggcgc gcgtgtgggg agcggcgctg gagcaccgcg aggcgctgtt
     2521 gcgggcgatg cggctgaccg gggcggccgc gctgtgcggt ctggcgctcg ccatcgtcgt
     2581 cggcagcctg acggcgtttg tcttctcgca gtcggtggtg atccagcggg gattttatcc
     2641 ctacgcgatc tttctgcaga cggtgccggt cgtggccatc gcgccgctga tcattctgtg
     2701 gttcggtcag gggtttcaga gcgtcgtgat cgtggcgttc atcctgagcc tgtttccgat
     2761 catcaccaat gggacgacgg gactgacggc gatcgaccgg ggccaccgcg agctgttcgc
     2821 gattcacaac gcgacgcgct ggcaggtgct ggttaaactg cggatcccga acgccgtgcc
     2881 ctacctcgtc acgggggcgc ggatttcgag cgggctgtcg gtgatcggag cgatcgtcgg
     2941 cgagttcttc gccgggttca gcgaccggaa tcgcggcctg gggtatctga ttcagttatc
     3001 gcacggccag ttgcggacgg cgtatctgtt cgcggcgatt ctggcctcga cgttgctggg
     3061 gctggcaatt ttcgcgctgg tcacgtggat cggcaatgcg atcgtcgccc gctggcgcga
     3121 ggcgtgacgc gcggagacgc aagagtcggg cggggttccg gccacccttt gccggggttc
     3181 ggggcgtcgg gggcggtgac gcagggttcc ggcctgcggc cgtcaccctg cgcttaaatc
     3241 cggtctccgc tgcgcggact taagcggtcc atcgacggcg gcgggccatg tcgattaatc
     3301 accgccttgt ttctggcatc cctcccggga tgcagggatg ttgagcggcc gaagaccggt
     3361 ggtatcgctt cgctcaacca ccggctacga tctggcatcc ctcccgggat gcgggggg
//
LOCUS       DAOPCO010000376         3359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22646_length_3359_cov_4.899213, whole genome shotgun sequence.
ACCESSION   DAOPCO010000376 DAOPCO010000000
VERSION     DAOPCO010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_3359_cov_4.899213"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLS90_16560"
     CDS             complement(<1..456)
                     /locus_tag="PLS90_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HPK04058.1"
                     /translation="MKTSITGKVYVIGDNIDTDQIIPAEHLVYSLTDPEERKLYGRYA
                     LSSVPPPQAGLPQGHVPFVRDGEFTSEYRIVIGGRNFGCGSSREHAPFALREAGIEAV
                     VAEFYARIFFRNCVNGGYLIPYESRERLIDLFKTGDEAEIDLATDTIRNL"
     gene            complement(576..1055)
                     /gene="rfaE2"
                     /locus_tag="PLS90_16565"
     CDS             complement(576..1055)
                     /gene="rfaE2"
                     /locus_tag="PLS90_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPK04059.1"
                     /translation="MRPKIHSRDELVALLQDRRRREPDFRLVFTNGCFDLLHIGHLRY
                     LWAARQLGSALLVAVNDDDSIRRLKGPGRPILALAERLELLAGFACVDYVTSFHEDTP
                     IPLLLLLKPEILVKGGNYTLDGVVGRDEVEAYGGAVRTVALTEGRSTTSLIDRMREQ"
     gene            complement(1094..1960)
                     /gene="amaP"
                     /locus_tag="PLS90_16570"
     CDS             complement(1094..1960)
                     /gene="amaP"
                     /locus_tag="PLS90_16570"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HPK04060.1"
                     /translation="MRLIRMLALIALQAIVIGLALDVLLSGVLTMGNRPLTHSLPVWL
                     QPNNRLTLKLFWSAALLVPLLYWIAAAASWKQRRRSIAVLSSDGEVMLLHPAALHKFV
                     RHQIETHPAVVSHKVDVRESGNKGIAISAVVNVEPIASLNAIKNDLQRSIRDGLEQVM
                     GISKIDDIRIVLGLDEKSITRRPGPERRPKARIEPPMRARRAAEATPAPSPPPASPAP
                     PVSEESGVIPMREVEETPAERDAAHGFGPPIEGDLPRPGSPADETTDKLSHDALRPDE
                     ARADAESPDQRP"
     gene            complement(1974..2375)
                     /locus_tag="PLS90_16575"
     CDS             complement(1974..2375)
                     /locus_tag="PLS90_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HPK04061.1"
                     /translation="MSVQEDLGRIQINNDVIATIASLAAVEVDGIVSVAGGSSLAEVW
                     GSKGMKKGITVTTDEATGNATIDLEVNVEYGVDVYKAAHQLQRSVKNAVESMTGLHVD
                     AVNVSISSIVLGERPRRPAALPAREAPSAED"
     gene            2924..>3359
                     /locus_tag="PLS90_16580"
     CDS             2924..>3359
                     /locus_tag="PLS90_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPK04062.1"
                     /translation="MGARRPIKTVNSICPIRINDLGGWTDTWFAEYGEVLNIGVYPYV
                     QCQMHVLPLDPDSENRVTIHAVDYGDRYGINPDKLIYDKHPLLEAAIDLMGVPRDLAV
                     EVSISSPVPGGCSTGTSAAVSVALIGALDRATYGRLTPAEVAM"
BASE COUNT          538 a          928 c         1305 g          588 t
ORIGIN      
        1 caggttgcgg atcgtgtcgg tggcgaggtc gatctcggcc tcgtcgccgg tcttgaagag
       61 gtcaatcagg cgctcgcgcg attcataggg gatcaggtag ccgccgttga cgcagttgcg
      121 gaagaagatg cgggcgtaga attcggcgac gacggcctcg atgccggcct cgcgcaaggc
      181 gaacggcgcg tgctcgcggg aggagccgca gccgaaattg cggccgccga tgacgatgcg
      241 gtattcgctg gtgaattccc cgtcgcggac gaaggggacg tggccctggg gcagcccggc
      301 ctgagggggc gggaccgagc tcagcgcata gcgcccgtag agcttgcgct cctcggggtc
      361 ggtcaggctg tagacgaggt gctcggcggg gatgatctga tcggtatcga tgttgtcgcc
      421 gatgacgtag accttgccgg tgatgctggt tttcatgggc cgggatcctg ggttgggcgt
      481 ggcgggattt gcggtcggga gcgacaccct gccccccagc gtagcaggcg gagggcgttc
      541 tgccaacgat gcgattgcgg ccgcgatcgt cgccgctact gctcgcgcat cctgtcgatc
      601 agagaggtgg tggagcggcc ttcggtcagg gcgacggtgc ggactgcgcc gccgtaggcc
      661 tcgacctcat cgcggccgac gacgccatcg agcgtgtaat tccccccctt gaccaggatt
      721 tcgggtttga gcaagaggag cagggggatc ggggtatcct cgtgaaacga ggtgacgtaa
      781 tcgacgcagg cgaagccggc caggagctca aggcgctcgg ccagcgcgag gatcggccgg
      841 ccgggcccct tgagccgccg gatcgagtcg tcgtcgttga cggcgacgag cagggcgctg
      901 cccaactggc gcgccgccca caggtagcgc aggtggccga tatggagcag gtcgaagcag
      961 ccgttggtga agaccaggcg gaagtcgggt tcgcggcgtc gccggtcctg gagcagggcg
     1021 acaagttcgt cgcgggaatg gatcttgggt cgcatgccgg ggaactgccg tgggattggc
     1081 gggcgagccg cgttcagggg cgttggtccg gggattcggc gtcggcccgc gcttcgtccg
     1141 gacgcagcgc atcgtggctg agtttgtcgg tcgtttcgtc ggcgggggaa ccgggtcgcg
     1201 gcagatcgcc ttcgatcggc gggccgaagc cgtgggcggc gtcgcgttcg gcgggggttt
     1261 cctcgacctc gcgcatcggg atgacgccgc tctcctcgga gacggggggg gcgggggacg
     1321 ccggcggagg cgagggggca ggggtcgcct cggccgcgcg gcgggcgcgc atcggcggct
     1381 cgatccgggc cttggggcgg cgttccggac cgggccggcg cgtgatgctt ttctcgtcga
     1441 ggcccaggac gatgcggatg tcgtcgatct tggagatgcc cattacctgc tccaggccgt
     1501 cgcggatcga gcgttgcagg tcgttcttga tcgcgttgag gctggcgatc ggctccacat
     1561 tgacgacggc cgagatggcg atgcccttgt tgcccgactc gcggacgtcg accttgtggc
     1621 tgacgaccgc gggatgggtc tcgatctgat ggcggacgaa cttgtgcagc gcggcggggt
     1681 ggagcagcat gacctcgccg tcgctgctca gcacggcgat ggaccggcgc cgctgtttcc
     1741 agctggcggc cgccgcgatc cagtagagca gggggacgag cagggcggcg gaccagaaca
     1801 gcttcagcgt gaggcgattg ttgggctgga gccagacggg cagggagtgg gtgagcgggc
     1861 ggttgcccat cgtgaggacg ccggacagga ggacgtcgag cgccagcccg atgacgattg
     1921 cctgcagggc gatcagggca agcatacgaa tcagtcgcat cgtcgtctcg ctttcagtct
     1981 tcggcggagg gcgcctcgcg cgcgggaagg gcggccgggc gtcgtgggcg ttcgccgagg
     2041 acgatgctgc tgatgctgac gttgacggcg tcgacatgca agccggtcat gctttcgacc
     2101 gcgtttttga ccgagcgttg caactggtgc gcggccttgt agacatcgac gccgtattcg
     2161 acgttgacct ccagatcgat cgtcgcgttg ccggtggctt cgtcggtggt gacggtgatg
     2221 cccttcttca tgcccttgct gccccagacc tcggccaggg aagagccgcc ggcgacgctg
     2281 acgatgccgt cgacttcgac ggccgcgagc gaggcgatcg tcgcgatgac gtcattgtta
     2341 atctggattc tgccgagatc ttcctgaacc gacatcgcca cgaggtctcc ctgggtgtca
     2401 cgcggggtca cggctcaaaa tgagccaaat cggtgccaga tcgaacgaag cggagccgcg
     2461 cggctcgggc gtggtgtcca actggtcacg gaccttcgcc tgtgaattaa catccgatat
     2521 ggccatttgc aagggaaacg aggaaggtgg ggatgcgagc gggatgagaa tcgcgggatc
     2581 gcgaccggta tcgctatcga gatcgtgatc gaagcggcgc ccgcagggcc cgctgcggcg
     2641 ggattgggct tggttcaccc gttgggctgt gataagtgat atcgttccgc atgcgggagg
     2701 agagggtccg agggaggcat tcaggatgcc gggggcggcg ccgcgggcgc tgggggatgg
     2761 ggagattttg ggaatgatgc ggaggctttc gcgagccaag gacgagggac gagagccgag
     2821 agccgaattc tggagaggac tttgggggcg atgacgggga cgagagacga gggacgatag
     2881 tcgagtgagg gacgacactt gatcgttgga gggttccagg cgaatgggcg cgcgacggcc
     2941 gatcaagacg gtcaacagta tctgtccgat ccggatcaat gatctggggg ggtggacgga
     3001 cacatggttc gccgaatacg gcgaggtgct caacatcggg gtgtatccct atgttcagtg
     3061 ccagatgcat gtgctgccgc tcgaccccga cagcgagaac cgggtgacga tccacgcggt
     3121 ggattacggc gaccgttacg ggatcaaccc cgacaagctg atctatgaca agcatccgct
     3181 gctggaggcg gcgattgatc tgatgggcgt gccccgggac ctggcggtgg aggtgagcat
     3241 ctcctcgccg gtgcccgggg ggtgttcgac ggggacgtcg gcggcagtct cggtggcgct
     3301 gatcggggcg ctggaccggg cgacgtacgg gcgcctgacg ccggccgagg tggcgatga
//
LOCUS       DAOPCO010000377         3358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22654_length_3358_cov_5.227975, whole genome shotgun sequence.
ACCESSION   DAOPCO010000377 DAOPCO010000000
VERSION     DAOPCO010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22654_length_3358_cov_5.227975"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1821)
                     /locus_tag="PLS90_16585"
     CDS             complement(148..1821)
                     /locus_tag="PLS90_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HPK04063.1"
                     /translation="MSPPRPELNYLNNGRSLWSWLTTTDHKRIALLYLFSITFFFALG
                     GLFAAGIRLELFTPEADLFTSDTYNRLFTMHGIIMVWFFLIPSIPTVFGNFLIPMMIG
                     ARDLAFPRLNLASWYVFMLGGLFTVIVMFIGGVDTGWTFYTPYSSTYSNSYVILAAAG
                     VFVVGFSSIFTGLNFIVSVHTLRAPGMHWFRLPLFVWSNYATSIIMVLATPVLAIVLV
                     LIALERAFGMPIFNPYKGGDPLLFQHLFWFYSHPAVYIMILPGMGVVSELVACFAHRR
                     IYGYVFVAWASLGIALLGFFVWGHHMFVSGQSMYAGFVFSFLSFMVAVPSAIKVFNWT
                     ATLHRGSITFHAPMLYALGSIGLFTVGGMTGLMLAAIAIDVHVHDTYFIIAHFHYIMV
                     GGMVMAYMGALHFWWPKMSGRLYPEWPAILAALIIFIGFNLTFFPQFILGYNGMPRRY
                     HVYPEEFQLLNVMSTAGASILGVGYVLPLFYLIWSFFKGKPAGPNPWDARGLEWEFAD
                     SPPIPNNFPATPLVTFAAYAYDRPDIRLLTGEQGQRFAIDAGPCAGETL"
     gene            complement(1818..2819)
                     /gene="coxB"
                     /locus_tag="PLS90_16590"
     CDS             complement(1818..2819)
                     /gene="coxB"
                     /locus_tag="PLS90_16590"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HPK04064.1"
                     /translation="MTSSLFQYLPRLLAQSRGPALIPIEASNIAPRTDLLFAAMLLIC
                     GGVTLAIAILIIFFSVRYRAGSAARRDRSPSTGYRLEIIWTLAPLGLFLLIFMWAAGI
                     FFQLHRRPAAAPLEINVMAKQWMWKFQHPGGQREIDELHLPVGRVVRLTMTSQDVIHS
                     FFVPAFRVKQDVLPDRFTTMTFAPTRPGMYYLFCAEYCGTDHSRMRGVVYVQQPREYE
                     LWLAARGAELDVALAGAAAFRRLGCSACHFADSGLQAPSLVGLWGRAVKLHDGAVVQA
                     DAQYLRDSILLPNKHRVAGYDPIMPSFQGVVTDDELFALVEYIKSLAAPAPAQGLQP"
     gene            complement(2816..>3358)
                     /locus_tag="PLS90_16595"
     CDS             complement(2816..>3358)
                     /locus_tag="PLS90_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04065.1"
                     /translation="PPPPEEPPLTAPRNTSHAEPTESTRHERDDAPARPLLIGLGVIV
                     LALIASMAGLAGLIRVFDRFIPRQELGPPHVLDLGPSAIPPAPTPPLLTSRRELDEAR
                     AADLALLSTYGWVDRDQRIVRLPIDRAMALVAERGLPAFAGDPASRDEFTTHAVRPAP
                     PRPVSPFLREALRGRGLDLP"
BASE COUNT          736 a          855 c         1289 g          478 t
ORIGIN      
        1 catatcccag gaacaggccg ccgaagagca ggagttcggt gacgagaaag atccacatcc
       61 ccatttcgta ggcgcggcgc tgctggagca ggtcgtcgaa ctggtgatgc ggcgccgtgg
      121 tccgggaggc gagttcgacg tgttccatca gagggtctcc cccgcgcaag gacccgcgtc
      181 gatggcgaag cgctggccct gttcgccggt cagcaggcgg atgtcggggc ggtcgtaggc
      241 ataggctgcg aaggtcacga gcggcgtagc gggaaagtta ttgggaattg gcggggaatc
      301 ggcgaattcc cactccaggc cgcgcgcgtc ccacggattg ggtccggcgg gtttgccctt
      361 aaaaaacgac cagatcaggt aaaacaaggg gaggacataa ccgacgccga gaatcgaggc
      421 gcccgcggtt gacatgacgt tcagcagttg gaattcctcg gggtagacgt ggtagcggcg
      481 cggcatgccg ttgtagccga ggatgaactg ggggaagaag gtcaggttaa atccaatgaa
      541 aataatcaag gccgccagga tggccggcca ttcgggatac aggcggccgc tcatcttggg
      601 ccaccagaaa tggagggcgc ccatgtaggc catcaccatg ccgccgacca taatgtaatg
      661 gaaatgggcg ataatgaagt aggtatcgtg gacgtggaca tcgatggcga tggcggccag
      721 catgaggccg gtcatgccgc cgacggtgaa caggccgata gatcccagtg cgtagagcat
      781 cggggcatga aaggtgatgc ttccgcggtg gagcgtggcg gtccagttga agaccttaat
      841 ggcggaggga acggcgacca tgaaactcag aaaggagaag acgaacccgg catacatcga
      901 ctggccgctg acgaacatgt gatggcccca gacgaaaaag ccgagcaggg cgatgccgag
      961 gctggcccag gcgacaaaga catagccgta gatgcggcgg tgcgcgaagc aggcgacgag
     1021 ttcgctgacg acgcccatgc cgggcagaat cataatgtaa acggcgggat gcgagtaaaa
     1081 ccagaacagg tgctgaaaga ggagcgggtc gccgcccttg taagggttga agatgggcat
     1141 gccgaaggcg cgttcgagcg cgatgagaac cagcacgatg gccagcaccg gagtggccag
     1201 gaccatgatg atgctcgtgg cgtagttgga ccagacgaag agcgggagcc gaaaccagtg
     1261 catgccgggc gcacgcagcg tgtggaccga aacgatgaag ttcagtccgg tgaagatgga
     1321 ggagaagccg acgacgaaga cgccggcggc cgccagaatc acataggaat tggaataggt
     1381 gctgctgtag ggggtataga acgtccaacc ggtatcgacg ccgccgatga acatgacgat
     1441 gaccgtgaac aggccgccga gcatgaagac ataccaactg gcgagattga ggcgggggaa
     1501 ggcgaggtca cgggcgccga tcatcatcgg gatgaggaaa ttgccgaaga ccgtcgggat
     1561 cgaggggatc aggaagaacc agaccatgat gatgccgtgc atcgtgaaga ggcggttata
     1621 ggtatcggag gtgaagaggt cggcctcggg ggtgaagagt tcgaggcgga tgccggcggc
     1681 gaaaaggccg ccgagggcga agaaaaaggt aatggaaaag aggtagagga gggcgatgcg
     1741 cttgtggtcg gttgtcgtca accacgacca gagtgagcgg ccgttgttaa gataattgag
     1801 ttcggggcgg ggagggctca tggttggagg ccttgcgccg gcgcgggcgc ggcgagcgat
     1861 ttgatatatt caaccagcgc gaacaattcg tcgtcggtga cgacgccctg gaaggacggc
     1921 atgatcgggt cgtagcccgc gacccggtgc ttgttgggga gcaggatgga atcgcggagg
     1981 tactgggcgt cggcctggac gacggcgccg tcgtggagct tgacggcgcg gccccacaga
     2041 cccacgagcg aaggggcctg caggccggag tcggcgaagt ggcatgccga gcagccgagg
     2101 cggcgaaagg ccgccgcgcc ggcgagggcg acgtcgagct ccgcgccgcg ggccgccagc
     2161 cacagctcat actcgcgggg ctgctggacg taaaccacgc cgcgcatgcg ggagtgatcg
     2221 gtgccgcaat attcggcgca aaaaaggtaa tacattccgg ggcgggtggg tgcgaacgtc
     2281 atcgtggtga agcgatcggg aaggacatcc tgcttcacgc ggaatgccgg aacgaagaag
     2341 ctgtggatga catcctgcga ggtcatggtg aggcggacga cgcggccgac ggggaggtgg
     2401 agttcgtcga tttcgcgctg gccgccgggg tgctggaatt tccacatcca ttgcttcgcc
     2461 atcacgttga tttccagggg agcggcggcg ggacggcggt gcagctggaa aaaaattccc
     2521 gccgcccaca tgaagatcag gagaaagagc ccgagcgggg cgagcgtcca gatgatttcg
     2581 aggcggtaac ccgttgaggg agaacgatcg cggcgcgcgg cggaacccgc ccggtagcgg
     2641 acgctgaaaa agataatgag gattgcgatc gcgagggtga cgccgccgca gatgaggagc
     2701 atggcggcga aaagcagatc ggtgcggggg gcgatgttcg acgcctcgat cgggatcagg
     2761 gccggtccgc gggactgcgc cagcaggcgg ggcagatatt ggaaaaggga cgaagtcatg
     2821 gcagatcgag tccgcggccg cgcagcgcct cgcgcagaaa gggggaaacg gggcgcgggg
     2881 gagccggccg gacggcgtgg gtggtgaatt cgtcgcgcga ggcgggatcg cccgcaaacg
     2941 cagggagccc gcgctcagcg acgagagcca tggcgcggtc aatcggcagg cgcacgatcc
     3001 gctggtcgcg gtcgacccag ccgtaagtcg agaggagggc aaggtcggcg gcgcgcgcct
     3061 catccagttc gcggcgactc gtgaggaggg ggggcgtggg ggccggggga atggccgagg
     3121 gcccgaggtc caagacgtgg ggcgggccga gttcctggcg cgggatgaag cggtcgaaca
     3181 cccggatcaa gccggccagg ccggccatgg aggcgatcag cgcaagcacg atgacgccca
     3241 gaccgatcag cagcgggcgc gcgggggcgt cgtcgcgctc gtggcgcgtc gattcggtcg
     3301 gctctgcgtg ggacgtgttg cgcggggcgg tcaatggggg ttcctcaggg ggcggggg
//
LOCUS       DAOPCO010000378         3356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22669_length_3356_cov_6.840351, whole genome shotgun sequence.
ACCESSION   DAOPCO010000378 DAOPCO010000000
VERSION     DAOPCO010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22669_length_3356_cov_6.840351"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLS90_16600"
     CDS             complement(<1..353)
                     /locus_tag="PLS90_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04066.1"
                     /translation="MTHRNFSQVEYFRQLAAAHRPRLAFDRAGLDFTAWRTALLAELH
                     TLLGPTPASAALNPEIVWEIEEDGLIKRRVLLDFEPGMSAAALVYIPRAALASPAPAI
                     LCSHGHGAYGKDSVMG"
     gene            complement(419..1594)
                     /locus_tag="PLS90_16605"
     CDS             complement(419..1594)
                     /locus_tag="PLS90_16605"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK04067.1"
                     /translation="MSRRKSKVTIKDVAFALGVNPSTVSLVMNGSRRISAPTRERVLK
                     VAREMGYIPDKRAQGLRFRRTRVLGIMVPALNEIYVPTVVDGAYRTARKAGYQLLIVY
                     SGQTAEEETDATMQLLAEGADGVMLIERRPDASGQPTEHPAYHLLLEHGVPTALLGTD
                     CPAGFNQVDIAMQSFVLQIMDAVLSRGRGASLITRHNGSLERFISEKQEQGFLEGMRK
                     AGVADPTRFIFSKCDHHELVELGRVAGQYLVENFGLRQAILTANDLIAEGVQNYVMEN
                     GIRTGGDFTFVSLSDLPYSPYGPTAFASAIVPMGQCGESLAELVIGQINAGSNWTPRR
                     RLFHPCPVTRHLERMDLSEEEKRELWNRQHEYRSRGFYQEFVRTESPVRAESTALPA"
     gene            2294..3169
                     /locus_tag="PLS90_16610"
     CDS             2294..3169
                     /locus_tag="PLS90_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04068.1"
                     /translation="MGYPITNLVDNVDTSEWVSDLSVGVGGGASVTFTWASPVAIGQV
                     SIFWTSPYMPREYTVDTWDGSQWVTHFHVTDTPAAAAYANHAYGFPASTTALRINIVQ
                     SHEANYPRFGEVNILTASEVPPLTDLMDLPVTLESSTDPTNFQGKTLVLDNDVNTDWV
                     SGLSVGVGGPATFTVTFPYAVKFSIVRIYWGFYPPEEFQVETWDEGASDWVVHDHITT
                     APPADDWTEHQDYYYAYHGDGNTMSGPDFPVETTKLRVNILVSSPDNFPRFSELEVLP
                     DPTKVPAELSDFTLE"
BASE COUNT          540 a         1128 c         1062 g          626 t
ORIGIN      
        1 cccatcaccg aatccttgcc gtaggcgccg tggccgtgcg agcagaggat cgccggcgcg
       61 ggggaggcga gcgcggcgcg ggggatgtaa accagcgcgg cggccgacat ccccggctcg
      121 aaatcgagca gcacgcggcg cttgatcagc ccgtcctctt cgatctccca gacgatttcg
      181 gggttgagcg cggcgctcgc gggggtcgga ccgagcagcg tgtgcagttc ggcaagcagc
      241 gccgtgcgcc aggcggtgaa atccaagccg gcccggtcga acgccaggcg cggccgatgg
      301 gcggccgcca gttggcgaaa atactccacc tgggaaaaat tacggtgggt catgccggag
      361 tttcgcgctc tttcgtgttg tttgtggttt tcgagatcaa aacttcaggg cttcgccctc
      421 aggccggcag cgcggtgctt tcggcccgca ccgggctttc ggttcgcacg aactcctggt
      481 agaaaccgcg gctgcggtat tcatgctgcc ggttccacag ctcgcgcttc tcctcctccg
      541 acaggtccat gcgctccagg tgacgcgtga ccgggcatgg atggaacagc cggcgccgcg
      601 gggtccagtt gcttcccgcg ttgatctggc cgatcaccag ctcggcgagc gactcgccgc
      661 attgccccat cggcacaatc gccgaagcga acgccgtcgg gccgtagggc gagtagggga
      721 gatcgctcag gctgacaaac gtgaagtctc ccccggtccg gatcccgttc tccatgacgt
      781 aattctgcac cccttcggcg atcaggtcgt tggccgtcag gatcgcctgg cgcaaaccga
      841 aattctcgac caggtattgc cccgccacgc ggcccagttc gaccagttcg tgatgatcac
      901 actttgagaa gataaaccgg gtcggatcgg cgacgcccgc cttgcgcatc ccctcgagga
      961 acccctgctc ctgcttctcg ctgatgaatc gctccagcga tccgttgtgg cgggtgatga
     1021 gcgaggcgcc ccggccgcgg ctcaggaccg cgtccatgat ctgcagcacg aacgactgca
     1081 tcgcgatatc cacctggttg aagccggcgg ggcagtccgt ccccagcagc gccgtcggca
     1141 cgccgtgctc cagcagcagg tggtaggcgg ggtgctcagt gggctggccc gacgcgtccg
     1201 gccgccgttc gatcagcatg acgccgtcgg ccccttcggc cagcaactgc atcgtggcgt
     1261 cggtctcctc ctcggccgtc tggccggagt agacgatcag cagttggtag cccgctttgc
     1321 gcgcggtgcg gtaggcgccg tcgaccacgg tcggcacgta gatctcgttg agcgccggga
     1381 ccatgattcc cagcacgcgc gtgcggcgga agcggagccc ctgggcgcgt ttgtcgggaa
     1441 tgtagcccat ctcgcgggcc accttgagca cccgctcgcg ggttggcgcg ctgatccggc
     1501 ggctcccgtt catgaccagg gagacggtgg aggggttgac cccgagcgca aaggccacgt
     1561 ctttgatggt gactttggat ttgcggcgcg acatgagaac tggcctcggg cagcgcaaac
     1621 tgggagggcg tgggacgcat gcacagcgcg agaaaagcgg agtccctgac cccgtgggag
     1681 gagtgtaagc gcgggggcgg ggggaattca acggcttttt gctcaaacgt ttgcaaggcc
     1741 atcgcgctcg cggcggcctg aacgcccgcg ggccggcttg gaacgccgcg ggacgacgtc
     1801 gcgggggagc gggacgctgc ggcggggtaa gcgctttctt tttcggttga ctttccgccg
     1861 ccgccgaccc tatacaatgg attgagcatt gagccgttgc ttattttcat cgttccgccc
     1921 ccccaaccgg ctggcatccc ggcgggttgg tagcccggtg gccgcacccg atccagagcg
     1981 gacgtcccgc ggaagccgta ttgtttcggt ccggggcgcg cgcggggacg aggctggagt
     2041 ttccagagaa aggagcgcaa tcgttcgact cgcgcttgac cccgcgtttc gattctcggc
     2101 ggcatggacg ccgcggctgt tggttatccc cattttactt tcctccaagg aggcactacc
     2161 tgccatgcgc tctttacttc ccacccgtcc cctaggcctg ttattcctcg ccggctggct
     2221 cctcgccgcc cctccgctgt gggcggggct cgaagactca gccaccctcg atgcgacccc
     2281 tcccggtaat ctcgtgggtt atcccattac gaacctggtg gacaatgtcg acacttcgga
     2341 gtgggtcagc gatctgagtg tcggcgtcgg cggcggcgcc tcggtcacgt tcacctgggc
     2401 cagcccggtc gcgatcggcc aggtctccat cttctggacg tcgccctaca tgccgcgcga
     2461 atacaccgtc gacacctggg acggctcgca gtgggtgacc cattttcacg ttacggatac
     2521 gcccgcggcg gccgcctacg ccaaccacgc ctacggcttc cccgcctcca ccaccgccct
     2581 gcggatcaac attgtccagt cgcacgaggc caactatccg cgcttcggcg aggtcaacat
     2641 cctgaccgcc tccgaagtgc ccccgctgac cgacctgatg gacctgccgg tcacgctcga
     2701 atcgtcgacc gatccgacca attttcaggg caagacgctc gtgctcgaca acgacgtcaa
     2761 caccgactgg gtgtccgggc tctccgtcgg cgtcggggga ccggccacgt tcaccgtcac
     2821 gttcccctac gcggtcaagt tctcgatcgt ccggatctac tggggattct atccacccga
     2881 ggagttccag gtggaaacct gggatgaagg cgcctcggac tgggttgtcc atgatcacat
     2941 caccacggcg ccccccgccg atgactggac cgagcatcag gattactact acgcctatca
     3001 cggcgacggc aacaccatga gcggccctga ttttccggtc gagacgacca agctgcgcgt
     3061 caacatcctc gtctccagtc ccgacaactt cccgcgcttc agcgaactgg aggtccttcc
     3121 cgatccgacg aaggtcccgg ccgaattgtc ggatttcacg ctggaataac ggtcagttcc
     3181 tttccctgtt cggggcggtc cgacgcgacc gccccgaaca ttttctcccg cgggtcaaat
     3241 cattccccca attcagaccg gatccggccg ccccttccag gggcttgaat cgcagggggc
     3301 ggcgacgcag ggttccggcc cgcggccgtc accctgcgct ttattccggt cgtccg
//
LOCUS       DAOPCO010000379         3333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_22866_length_3333_cov_3.754118, whole genome shotgun sequence.
ACCESSION   DAOPCO010000379 DAOPCO010000000
VERSION     DAOPCO010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22866_length_3333_cov_3.754118"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="PLS90_16615"
     CDS             <1..758
                     /locus_tag="PLS90_16615"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPK04069.1"
                     /translation="AYGKDSVMGVASLNDPARQQEIRRLNYDYGLQMARRGYVTIAID
                     YRGFGERGDPATPHGASSYDVPYPGRDKCNVHFIRGSMLGFNLLALDIFDARRTLDYL
                     TSLECVDAARIGAMGLSFGGVMTTWTALLDERIRAAAILAYSTTFQTMIATGNICGSQ
                     YLPGLYALCDLADLQGAIAPRPLLASFGTIDSCFAIDDALACGRRVRAIYAAADAAER
                     YEEEIHEGDHVFSGRKPFEFFDRHLGPAAPAPV"
     gene            789..2039
                     /locus_tag="PLS90_16620"
     CDS             789..2039
                     /locus_tag="PLS90_16620"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK04070.1"
                     /translation="MAPLRIGYIGVGMRSLGFTDFIKRHPGEFELVAVAELNLDKARV
                     VLDHFQLEAELMEDHEALTARDDLDAVMVLTPDYAHADPAISAIRNGKHVFIEKPLAT
                     TLADCDRVIAAAEGTDTVVYIGFNMRHTPVHEKIFNLIRSGELGRVTTIEANEWYNGG
                     KTYFRRWHRLRRFGGGLWLTKACHDFDLVTWIAGGRPTSIYAVDSLSHYRHREGVGPR
                     CRDCAEKATCPDYYDINKPYEHPLMETYRNMQLQMDQSVEWAPDICLWNSAKDTFDNG
                     MAILTYDNDIRASYTVNVLGARTTRQMKVVGTLAMVEGDLEEGIVKYYHRHDEGRNRV
                     YDLNAEIEGGHGGADERLMRDFLHCCRTGAAPRSGLAEGRLAVELSTAATRSADLGQV
                     IHLNGRGPQPAGAAIQNNAAARPR"
     gene            complement(2005..>3333)
                     /locus_tag="PLS90_16625"
     CDS             complement(2005..>3333)
                     /locus_tag="PLS90_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04071.1"
                     /translation="VDAASNTIRLTQPDGTRAEFAVAEAQLGPTRMLANPVPGGAPMH
                     DARPTNFRPRVADIDGDGRPEILLATEVATRTLILTAGDRPGAPPADLREDTSGQLLL
                     VYDYDRDGTNERLYLLAHPTGSRMRLLDARDQTLWETVFTGAPQTVNQYTYGDFTGDG
                     IEDICLTFNEYALRPEADYPIFTINNEYARIATWNGANGQFMWFGSIIADERFYGYGG
                     YPVVYDFNGDGCDDIISNTPEVRVINSGIDGKLLYDYRYDKSFWYCWGAYSIPYLDDF
                     DGDGRLDLFSQGSNYGVALATLPDRPLWLDELADQGLYGNLVDSRFQGVADVDGDGHL
                     EIGVGVCQGEFRCYDGADGTIQWRVPMASPTTEVCTADVDGDGLYEFIFGTEAGELYA
                     LGGPAGTEDPIKWRVELGAPLSPPTVADVDGDGLGEILVISGDGLLRCFE"
BASE COUNT          586 a         1117 c         1084 g          546 t
ORIGIN      
        1 gcgcctacgg caaggattcg gtgatgggcg tggcgtcact gaacgatccg gcgcgccagc
       61 aggaaatccg ccgcctcaac tatgattacg gcctgcagat ggcgcggcgc ggttacgtga
      121 cgatcgcgat cgactaccgc ggattcggcg agcgcggcga cccggcgacc ccgcatggcg
      181 catcgtcata cgacgtgccg tatcccggcc gcgacaagtg caacgtccac ttcatccgcg
      241 gctcgatgct gggcttcaac ctgctcgcgc tcgatatttt cgacgcgcgg cgcacgctgg
      301 actatctgac cagcctggag tgcgtcgacg ccgcgcgcat cggcgcgatg gggctgagct
      361 tcggcggcgt gatgaccact tggacggcgc tgcttgacga gcgcatccgc gcggccgcaa
      421 tcctggccta cagcacgacg ttccagacca tgatcgcgac gggcaacatc tgcggctcgc
      481 agtatctacc ggggctgtat gcgctatgcg atctcgccga tctgcaggga gcgatcgcgc
      541 cgcggccgct gctggcctcg ttcggcacga tcgactcgtg cttcgccatc gacgacgcgc
      601 tggcgtgcgg acgccgggtc cgcgcgatct acgcggcggc ggacgcggcc gagcgctatg
      661 aagaagaaat ccatgaaggc gaccacgtct tctccggacg caagccgttc gagtttttcg
      721 accgccatct gggcccggct gccccagcgc ccgtctgaac aacgcacaca ccgcttaagg
      781 catccatcat ggcaccgctt cgcatcggct acatcggcgt cggcatgcgc tcgctgggct
      841 tcaccgattt catcaaacgc catcccgggg aatttgagct ggtggctgtc gccgagctca
      901 atctcgacaa ggcgcgcgtc gtcctcgatc acttccagct cgaggccgag ctgatggagg
      961 accacgaggc cctgacggcg cgcgacgacc tcgacgcggt gatggtgctg acgccggact
     1021 acgcccacgc cgaccccgcc atctcggcca tccgcaacgg caagcatgtg ttcatcgaaa
     1081 aacccctggc gacgacgctg gccgattgcg accgcgtcat cgcggcggcc gaggggaccg
     1141 acacggtcgt ctacatcggg ttcaacatgc gccacacgcc ggttcacgaa aaaatcttca
     1201 acctgatccg gtcgggcgaa ctgggccggg tgacgacgat cgaagccaat gagtggtata
     1261 acggcgggaa gacctacttc cggcgctggc atcggctgcg ccgcttcggc ggcggcctct
     1321 ggttgaccaa ggcgtgccac gacttcgatc tggtcacctg gatcgccggc ggacggccca
     1381 cctcgattta cgccgtcgac agcctctccc actaccgtca tcgcgagggg gtcggcccgc
     1441 gctgccgcga ctgcgcggaa aaggcgacct gccccgacta ctacgacatc aacaaaccct
     1501 atgaacaccc gctgatggaa acctatcgca acatgcagtt gcaaatggac cagagcgtgg
     1561 agtgggcgcc ggacatctgc ctgtggaact cagcgaagga tacgttcgac aacggcatgg
     1621 cgatcctgac ctatgacaac gacatccggg caagctacac cgtgaacgtc ctcggggcgc
     1681 gcacgacccg tcagatgaaa gtggtcggca cgctcgccat ggtcgagggc gacctggagg
     1741 aagggatcgt caagtattac caccggcatg atgagggcag gaaccgggtc tacgacctga
     1801 acgccgaaat cgaggggggc cacggcggag ccgatgagcg gctgatgcgc gacttcctcc
     1861 actgctgccg gacgggggcc gcgccccgct ccggtctggc cgaaggccgg ttggccgtgg
     1921 aactgtcgac ggcggcgacg cgctcggccg acctcggcca ggtcatccat ctgaacggac
     1981 ggggcccgca acccgccggc gcggctattc aaaacaacgc agcagcccgt ccccgctgat
     2041 caccaggatc tcgcccagcc cgtcgccgtc cacatcggcc acggtcgggg gcgagagcgg
     2101 ggcgccaagt tccacccgcc acttgatcgg atcctccgtg cccgccggac cgcccagggc
     2161 gtaaagctcg cccgcctccg ttccaaagat gaactcatac agtccatcgc catcgacgtc
     2221 ggccgtgcac acctcggtcg tgggcgacgc catcggcacg cgccattgga tcgtcccgtc
     2281 ggccccgtcg taacagcgga attctccctg gcagacgccg accccgatct cgagatgccc
     2341 gtcgccatcg acgtcggcca cgccctggaa ccggctgtcg accaggttgc cgtaaaggcc
     2401 ctgatcggcg agctcgtcca gccacagcgg ccggtcgggc aacgtggcga gggcgacgcc
     2461 gtaattgctc ccctggctga acaggtcgag ccgcccgtcg ccgtcaaagt cgtccaggta
     2521 ggggatgctg taggcgcccc agcaatacca gaagctcttg tcgtagcgat agtcatacag
     2581 cagcttgccg tcgatccccg aattgatcac ccgcacttcg ggggtgttgc tgatgatgtc
     2641 gtcgcagccg tcgccgttga agtcataaac gacgggatag ccgccgtaac cgtagaagcg
     2701 ttcatcggcg atgatgctgc cgaaccacat gaactggccg ttggccccgt tccacgtggc
     2761 gatgcgggca tactcgttgt tgatcgtgaa gatcggatag tcggcctcgg ggcgcagcgc
     2821 gtattcattg aacgtcaggc agatgtcctc gatgccgtcg ccggtgaagt cgccgtaggt
     2881 atactggttg accgtttgcg gcgcgccggt gaagaccgtc tcccagaggg tctggtcgcg
     2941 ggcgtcgagc agccgcatgc ggctgcccgt cgggtgcgcc agcaggtaga gccgctcgtt
     3001 cgtgccgtcg cgatcgtaat cgtagaccag cagcaactgg ccggaggtgt cctcgcgcag
     3061 atccgcgggc ggcgcacccg gccgatcgcc cgccgtcagg atcagcgtgc gcgtggcgac
     3121 ttcggtggcc aggaggatct cgggccgccc atcgccgtcg atgtcggcca cgcgggggcg
     3181 gaaattggtc ggccgcgcgt catgcatcgg ggcgccgccg ggaacggggt tggccagcat
     3241 ccgcgtgggg cccagctgcg cctcggcgac ggcgaactcg gcgcgcgtgc catcgggctg
     3301 ggtcagccgg atcgtgttgc tcgcggcgtc gac
//
LOCUS       DAOPCO010000380         3277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23328_length_3277_cov_4.676288, whole genome shotgun sequence.
ACCESSION   DAOPCO010000380 DAOPCO010000000
VERSION     DAOPCO010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23328_length_3277_cov_4.676288"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1996)
                     /locus_tag="PLS90_16630"
     CDS             complement(<1..1996)
                     /locus_tag="PLS90_16630"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HPK04072.1"
                     /translation="MSRKFISTLLLLMVAWLGWETAVPAQLKPYGLPDQPHARAELLA
                     DVTAIEPGRPFALGIRYEMDPEWHIYWRNSGDVGLRTQIDWILPEGFAVGPLQWPVPE
                     RVEAGGLISYAYHDEVILFAEVTPPPDLTAGEPVTLRAHSEWLVCRVECVPGEADLEL
                     TLPVGEPSPSAAAPLFAAARARLPEPVLLAPQAGPLDEDLPLAIELADENPAPAPGET
                     RPIELRLSPEPPWQFAAETAGPAAAEIFPLVPARDWDAQHPSRTAADASRLVFRWSPM
                     VRKQAKGHAAPANLAEAGDEDLLRIVARIPLVNPETGEERLELFTVALPATIAAGTAE
                     AGAVAEPAVDGDPAAEAATPTAAGAEPESADRDGLSFLSAPGEGPARRMSLGLLLLYA
                     FLGGMLLNVMPCVLPVLSLKVLSFMRQGGEAHGRVLRLGLAFGAGVLASFGALALVVI
                     LLSLGGQRVGWGFQMQEPRFVIALAMIVLAFALSLFGVYSIELPGQSATKLDAAGRRE
                     GYPGAFMNGVLITALATPCTAPLLGPAIGLAFALPPPVTLTAFLAAGAGLAAPYVLLS
                     AFPAWTRWLPRPGAWMETFKQAMGFLLLATLVWLLSVLTSLVDPSTFVRTLVLLLVVG
                     FACWLWGLGTSPLAGLPRRATTMIAVPLLVAAAYYLLPERA"
     gene            complement(2207..>3277)
                     /locus_tag="PLS90_16635"
     CDS             complement(2207..>3277)
                     /locus_tag="PLS90_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK04073.1"
                     /translation="AGFGRKCKVLAPHTSEEYASFYEGTGDAIFRNIEFIERFSNVSE
                     VLILSGDHIYRMDYRPMLELHRAKGAAVTIATQEVPWEETSRFGLMISDEQGIIRQFQ
                     EKPKSHPLSNRASLGIYIFNREALVEYLQRDQEDPDSNHDFGGDIIPRIIYAETACEY
                     PFDGYWRDVGTIQSYYDTSMEALDPESGLELARWGVRTNHQQIPLAHQHPARLARGAR
                     VERSIISKGTVIEGEVIDSVLSPGVRVAAGARVHRSILLHNVQVGEGCHLDNVIVDKH
                     GVLERDVRIGDPELGDQVNQHKPELLFTGTSVIGKNSRIGRGVRIGRNCLIFPQVVVE
                     EAPGNTIESGRTIFFAEVGEHV"
BASE COUNT          545 a         1184 c         1076 g          472 t
ORIGIN      
        1 gcgcccgttc cggcagaagg tagtaggccg ccgccacgag cagcggcacg gcgatcatcg
       61 ttgtcgcgcg ccgcggcaga ccggccagcg ggctcgtccc cagcccccac agccagcacg
      121 cgaagcccac caccagcagc aggaccaggg ttcgaacgaa cgtggagggg tcgacgaggc
      181 tggtcagcac gctcagcagc cagacgagcg tcgccagcag caggaaaccc atcgcctgct
      241 tgaacgtttc catccacgcg cccggccggg ggagccagcg ggtccatgcc ggaaaggccg
      301 acagcagcac atagggggcg gccaggccgg cgcccgccgc cagaaacgcg gtcagggtca
      361 cgggcggcgg cagggcgaac gccagcccga tcgccgggcc cagcagcggc gccgtgcagg
      421 gcgtcgccag cgcggtgatc agcacgccat tcatgaacgc gccggggtag ccctcgcgcc
      481 ggcccgccgc gtccagtttc gtggcgctct ggcccggcaa ctcgatgctg taaacgccaa
      541 acaggctcaa ggcgaacgcc agcacgatca tcgccagcgc gatgacgaac cgcggctcct
      601 gcatctgaaa gccccacccc acgcgctggc cgcccagact cagcaggatg acgaccagcg
      661 ccagggcgcc gaaggaggcc aggacgcccg cgccgaacgc caggcccagc cgcaggacgc
      721 gcccgtgcgc ctcgcccccc tggcgcatga aactgagcac cttgagcgac agcaccggca
      781 gcacgcatgg cattacgttg agcagcatgc cgcccagaaa ggcgtaaagc agcagcagcc
      841 ccagcgacat ccgccgggca gggccctcgc ccggcgccga gaggaaactc agcccgtccc
      901 ggtcggcgct ttcgggttca gcgccggcgg ccgtcggggt cgccgcctcc gccgccgggt
      961 cgccgtccac cgccggctcc gcgaccgcgc cggcttcggc cgtgccggcc gcgatcgtcg
     1021 ccggcagggc cacggtaaac agttccagcc gttcctcgcc ggtctcgggg ttgaccagcg
     1081 gaatccgcgc gacgatgcgc agcagatcct catcgcccgc ctcggccaga ttggccggcg
     1141 cggcatgccc cttggcctgc ttgcgaacca tgggactcca gcggaacacc agccgcgagg
     1201 cgtcggcggc ggtgcggctt ggatgctggg catcccagtc gcgagccggc acgagcggga
     1261 agatctctgc cgcggcggga cccgccgtct ccgcggcgaa ttgccacggc ggttccggac
     1321 ttagccgcag ctcgatcggc cgcgtttcgc cgggggccgg cgcggggttc tcatccgcga
     1381 gctcgattgc gagcgggagg tcttcatcca gcggaccggc ctggggcgcg aggaggaccg
     1441 gttcggggag ccgcgcgcgc gccgcggcaa acaggggcgc cgccgccgac ggactcggct
     1501 cgcccaccgg cagcgtcagc tccagatccg cttctcccgg gacacactcc acccggcaga
     1561 ccagccactc cgagtgcgcg cgcagcgtca ccggctcgcc ggccgtcagg tccggcggcg
     1621 gggtgacctc cgcaaacaga atcacctcat cgtgataggc gtagctgatc aggccgccgg
     1681 cttcgacccg ctcggggacg ggccactgca gcggtccgac cgcgaaaccc tcgggcagaa
     1741 tccagtcaat ctgcgtccgc aggccgacat cgccgctgtt gcgccaatag atgtgccact
     1801 ccgggtccat ctcataccgg atgccgagcg cgaacggccg gcccggttcg atcgcggtca
     1861 cgtcggccag cagttcggcc cgggcatggg gctgatccgg caggccatat ggcttgagct
     1921 gggccgggac cgctgtttcc cagcccagcc aagccaccat cagcaggagg agcgtgctga
     1981 tgaactttcg cgacatcgag cggcgaggcg atggattcgg cgcaccgatt ccgagaaatc
     2041 cgttcatgag caacttccag accagcattt gcatgttgtc agcgcggcgg ccggatcctg
     2101 gcgcctcatc catcggtcgg gtcggcgggg cgcagcctga cagcccccct tggatcatct
     2161 agacgttcca tcgcgcggga tgttcccgat ccgccccgcg cccggctcag acgtgttcgc
     2221 ccacctcggc aaagaagatc gtccggcccg actcgatcgt gttgccgggc gcctcctcca
     2281 ccacgacctg cggaaagatc aggcagttgc gcccgatgcg cacgccccgg ccgatccgcg
     2341 aattcttgcc gatcacgctc gtgccggtga acagcagttc cggcttgtgc tggttgacct
     2401 gatcgcccag ctccggatcg ccgatccgca catcgcgctc cagcacgccg tgcttgtcca
     2461 cgatgacgtt gtccaggtgg cagccctcgc ccacctgcac gttatgcagc aggatcgagc
     2521 ggtgaacccg cgcccccgcg gcgacccgca cacccggcga gaggaccgag tcgatcacct
     2581 cgccctcgat caccgtcccc ttgctgatga tcgagcgctc gacgcgcgcg ccgcgcgcca
     2641 ggcgcgccgg atgctgatgc gccaacggga tctgctggtg attggtgcgc acgccccagc
     2701 gggccagttc cagccccgac tcgggatcga gcgcctccat cgacgtgtcg tagtaactct
     2761 gaatcgtgcc gacgtcgcgc cagtagccgt cgaacgggta ttcgcaggcg gtctccgcat
     2821 agatgatccg cgggatgatg tcgccgccga aatcgtggtt cgaatcgggg tcttcctggt
     2881 cgcgctggag atactcgacc agcgcctcgc ggttgaaaat gtagatcccc agcgaggcgc
     2941 gattcgacag cggatggctc ttcggcttct cctgaaactg gcggatgatc ccctgctcgt
     3001 cggagatcat caggccgaag cgcgacgtct cctcccacgg cacctcctgg gtggcgatcg
     3061 tcaccgccgc ccccttggcg cggtgcagtt cgagcatcgg ccggtaatcc atccggtaaa
     3121 tatgatcgcc cgagaggatc aggacctcgc tgacgttgga aaaccgctca atgaactcga
     3181 tgttgcggaa gatcgcgtcg ccggttccct cgtagaacga ggcgtattcc tcgctggtgt
     3241 gcggcgccag caccttgcac ttgcggccga agccggc
//
LOCUS       DAOPCO010000381         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23467_length_3264_cov_2.359302, whole genome shotgun sequence.
ACCESSION   DAOPCO010000381 DAOPCO010000000
VERSION     DAOPCO010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23467_length_3264_cov_2.359302"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..271
                     /locus_tag="PLS90_16640"
     CDS             53..271
                     /locus_tag="PLS90_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04074.1"
                     /translation="MNNHNTVLDTRRDHRLKELAELFSKGFLRLSKTPEWREQAALAA
                     PSRQMLTGQKQSAQSGQKTSGKSSELTG"
     gene            346..852
                     /locus_tag="PLS90_16645"
     CDS             346..852
                     /locus_tag="PLS90_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HPK04075.1"
                     /translation="MKLCLEKELESLKEMTVSQLQRRYAEVFGEPTRAHNKPFLWKRI
                     AWRLQMQAEGDISERARRRAAELAREEDLRIRAPKGAFAPSSPADKARTMVLPFKTAK
                     RDERLPAPGTMLGRIYKGRRVEVEVLEKGFRFKGRNYPTLTAVAQEITGTHWNGYEFF
                     GLRKRADS"
     gene            849..2477
                     /locus_tag="PLS90_16650"
     CDS             849..2477
                     /locus_tag="PLS90_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009094856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HPK04076.1"
                     /translation="MNATQPESRRGTSEREQPRTIRCAIYTRKSTDEGLDQEFNSLDA
                     QRESAEAYIASQRLEGWTLLPERYDDGGFSGGNMERPALKRLMADAEAGLIDCLIVYK
                     VDRLSRSLMDFARIMEILDRRNVSFVSVTQQFNTTNSMGRLTLNILLSFAQFEREIIA
                     ERTRDKMSAARRKGKWVGGMPVLGYDVDPRGGRLLVNEEEAAQVREIFEIYLQEQGLV
                     TAAQELARRGLVTKRWVTKKGIERGGHLFNKNSLFGLLTNIIYVGKVDYKGSIYDGEH
                     PAILGEGIWRRVQERLRHNGRSGGVEVRNKYGGLLKGLIFCEACHAAMIHTWTVTAKN
                     KRRYRYYVCSHAQKHGWDTCPTKSLPARDIEQFVVGRIRAIGQDDALLEETLRAARNE
                     NEDRLRTLQDEERALLRQLRELGAEERKLAKDIGKDNGHTGAMTESLSRLQTRMREAH
                     ERATGARNEIIAVSGQLVSEREMRLAFSLFDPIWDSLTPKEQTRAMRLIVERVGYDPT
                     EGAVSILFQPTGIRALAEQVNQSEEACEDIDKEQ"
     gene            2483..2917
                     /locus_tag="PLS90_16655"
     CDS             2483..2917
                     /locus_tag="PLS90_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04077.1"
                     /translation="MEKLKIVAHVHFGREKRGKRRLLAGRAPQPERQMEQGNIPRLAR
                     LMALAIHFEGLLARGEVKDMAELARLGHVTRARITQIMNLRLLAADIQEALLDLPRTM
                     KGRDRLQYRHVQPMTVTLSWGKQREMWAMLDGRFPAAADHKR"
     gene            complement(2918..>3264)
                     /locus_tag="PLS90_16660"
     CDS             complement(2918..>3264)
                     /locus_tag="PLS90_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04078.1"
                     /translation="TGLATAEFMGLQQIERGSTAIVEIRTNAPATPGVYRTAWGLRAG
                     STPFGEGAETWVQVVPCAADASEAATLIDVLLGRISIQSLAPGSLDLNGDGKIDVADL
                     VTMMNRTGAEFD"
BASE COUNT          728 a         1013 c         1000 g          523 t
ORIGIN      
        1 gggcggccat tcccaaccac agcgcctccg gaactgaacc aggagtaagc tcgtgaacaa
       61 ccacaacacc gtgctcgata cgcgaagaga tcatcgtctc aaggaactcg cggaactgtt
      121 ctccaaggga ttccttcgtc tctcgaaaac gcctgaatgg cgtgagcagg cggctctggc
      181 cgctccatcc cgccagatgc tcacaggcca gaagcagtcc gctcagtcag gtcagaaaac
      241 ctcgggcaag agcagcgaat tgactggatg agttcgcccc gcccaagcat tcatggaccc
      301 cgtggttaac agtgttcaaa aaccactgaa aggagagaca aacaaatgaa actgtgcctc
      361 gaaaaggaac tcgaatcgct caaggaaatg acggtctcgc agctccagcg cagatatgcc
      421 gaagtcttcg gcgaacccac gcgcgcccac aacaagccgt tcctctggaa gcgcattgcc
      481 tggaggctcc agatgcaggc ggagggggat atcagcgaac gtgcccgcag acgggcagcg
      541 gaacttgccc gcgaagagga cctgcgcatc cgcgcgccca aaggcgcctt cgcgccctcg
      601 tcgcccgccg acaaggctcg aacgatggtt ctgccgttca agactgccaa gcgcgatgaa
      661 cgacttcccg ctcccggcac gatgctgggc cgcatctaca aaggcaggcg cgtggaagtg
      721 gaagttctgg aaaagggctt ccgattcaaa ggaaggaact acccgaccct gacggctgtt
      781 gcccaggaga tcacgggaac gcactggaat ggctatgaat tcttcggcct gagaaagagg
      841 gcggacagtt gaacgcgacg caacctgaga gtagacgagg gacatccgag agggagcagc
      901 cgcgcacgat ccgctgcgcg atctacaccc gcaagtccac cgacgaggga ctcgatcagg
      961 aattcaactc gctcgacgcc cagcgcgaat ccgccgaagc ctatatcgcc agccagcggc
     1021 tcgaaggctg gacgctgctg ccggagcgtt acgacgatgg cggcttctcg ggcggcaaca
     1081 tggaacgccc ggcgctcaag cggctgatgg cggatgccga ggccggattg atcgactgcc
     1141 tcatagtcta caaggtcgac cgcctcagcc gctcgctgat ggactttgca cgcatcatgg
     1201 aaatcctcga ccgccgcaac gtctccttcg tctccgtaac gcagcagttc aacacgacca
     1261 actcgatggg gcgactcacg ctcaacatcc tcctgtcctt cgcgcagttc gaacgcgaga
     1321 tcatcgccga acgaacgcgc gacaagatgt ccgccgcccg gcgcaagggc aagtgggtcg
     1381 gcggcatgcc ggtgctcggt tacgatgtcg atcctcgcgg tggccggctc cttgtcaacg
     1441 aggaggaggc cgcgcaggtg cgcgagatat tcgagatcta ccttcaggag caggggctcg
     1501 tcaccgcggc gcaggaactg gcccgtcggg gcctcgtcac caaacgctgg gtgacgaaga
     1561 agggcattga gcgcggcggt catctattca acaagaacag cctgttcggc ttgctgacca
     1621 atatcattta cgtcggcaag gtggactaca aagggtccat ctacgacggc gaacatccgg
     1681 caatcttggg cgaagggatc tggcggcgtg tgcaggagcg gcttcgccac aacggtcgat
     1741 cgggcggagt agaggtccgc aacaagtatg gcggcctgct caagggcctc atcttctgcg
     1801 aggcgtgcca cgccgcgatg atccacacat ggaccgtcac ggccaaaaac aaacggcgct
     1861 accgctacta cgtctgttct cacgcgcaga agcacggttg ggacacctgc ccgaccaagt
     1921 cgctgcccgc gcgcgatatt gagcagttcg tcgtggggcg catccgggcc atcggccagg
     1981 acgacgcgct gctagaagag acgctccggg cggcgcgcaa cgagaacgaa gatcggcttc
     2041 ggacgctcca ggacgaggaa cgtgcattgc tcaggcagct tcgcgaactc ggtgcggaag
     2101 agcgcaagct cgccaaggat atcgggaaag ataacggcca tacgggagcc atgaccgaat
     2161 cactcagccg cctgcagacc cgcatgcgcg aagcacatga gcgtgcaacc ggagcgcgca
     2221 acgagatcat tgcggtctcc ggccagcttg tgtccgaacg ggaaatgcgg ctggccttct
     2281 cgctcttcga tcccatctgg gactcgctca cgcccaagga gcagacccgc gcgatgcgcc
     2341 tgatcgtcga acgcgtcggc tacgacccca ccgaaggcgc cgtctcgatc ctgttccagc
     2401 cgaccggtat ccgcgccttg gccgagcagg tcaatcaatc tgaagaagca tgcgaagaca
     2461 tcgacaagga gcaatgagca gcatggagaa actcaagatc gtcgcccacg tccacttcgg
     2521 gcgcgagaaa cggggcaaac gccgccttct ggccggcaga gcgccccagc ccgaacgaca
     2581 gatggaacag gggaacatcc cgcgcctggc gcgcctgatg gcccttgcga tccacttcga
     2641 ggggttgctg gcacgcggcg aggtcaagga catggccgaa ctcgcccgcc tgggtcacgt
     2701 cacccgcgcc cgcatcaccc aaatcatgaa cctccgcctc ctcgccgccg acatccagga
     2761 agccctgctt gacctaccac ggaccatgaa ggggcgggac cggttgcaat ataggcacgt
     2821 tcaaccgatg accgtgacat tgagttgggg aaagcaacgg gagatgtggg caatgcttga
     2881 tggtcgattt cctgccgcag ctgatcataa acggtaatca atcaaattct gcgccggttc
     2941 gattcatcat ggttaccaaa tcagccacat cgattttgcc gtcgccgttc agatcgagtg
     3001 atccgggggc aagcgactgg atggaaatcc ggccgagaag gacgtcgatc agtgttgcgg
     3061 cttctgaggc atcggcggcg caaggcacga cctgcaccca tgtttcggcg ccctcgccga
     3121 agggtgtcga gccggcgcgc aaaccccagg ccgtccggta gacgcccggc gtggcggggg
     3181 cattggtgcg gatctcgacg atggcggttg agccgcgctc gatctgctgg agacccatga
     3241 attcggccgt ggcgaggccc gtca
//
LOCUS       DAOPCO010000382         3253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23565_length_3253_cov_3.202314, whole genome shotgun sequence.
ACCESSION   DAOPCO010000382 DAOPCO010000000
VERSION     DAOPCO010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23565_length_3253_cov_3.202314"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..2456)
                     /locus_tag="PLS90_16665"
     CDS             complement(171..2456)
                     /locus_tag="PLS90_16665"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPK04079.1"
                     /translation="MSIDANSGALTYLSPILPDWGFSHSMPRAELADGRLSDTRTLAP
                     PSIDQSTAGPETLWKIQWLDPDLGLRFDQNVQTLEAGQSLLIATSLTNVGDRPLALEA
                     VHALDVNGQGNSRIHYQGDWRPFTIYTDNNGSTWTGVKRLDCFVSPLTYTPEEGQGMV
                     SFSIRTGRIHESWGGVSVLYDGARQAALACCALSWNRALTNIRLAIGDDGPLRLDARS
                     HFAGLAIQPGETVHTEPLWIASDRDPLRLLERYGDEVKARNQPLIWPYIPMGFMSWYA
                     TRLQGGADLVLRNADFVKQRFADYPFEYCHWGHGWQADNSMGNWVAHAEKNPNGMKWL
                     ADELDKRGMKLGIFAAFLLVTDDVPLFREHPDWLLREKDGQLSSSWLGIWQREVNPRV
                     KHDGTVYFLDGTHPEVQDFLRQTIRRLKSEGVKYFLFDFFGPYSSDRVFHDKTRVPGN
                     EALRATAQAQALELDPSDFVYYCSAPTMSFVGLAHCVNCAADIGGPGSHLDEGFVSAI
                     SCIGARWYAHRRLWLTQPDALCVGLPGTLSEARVRTALVGLCGGIFNLSEDLRDVPEE
                     RLRIAETAMPPYGVAARPLDLFTNPYPGGYPQVWHLPVETDWDRWDVVGLLNAEDRER
                     TFTVDPAEFAAESHSPCWVHEFWTSQDLGQPAGPLQITVPPRDTRLLVFRPRRPHPWV
                     IATDMHLTAGAMEIQQATWDADNLTLDLVLSRRRGADGALFIAVPDGYTPTEGTVLNG
                     RLALKVDFPTKEITRRLHFTR"
BASE COUNT          585 a          765 c         1392 g          511 t
ORIGIN      
        1 gggagaagcg agaagagaga agagagaggg gagaagaaag aaaagtgatg gacacgaaag
       61 acacgcaagg acacgaaagg ggaaggaaag gagaggaccg gcgagggcgc cggtcccaca
      121 cgggcgatag cgagggcgat agcgatcgcg atccggatac cgattgagaa ctagcgggtg
      181 aaatggaggc ggcgggtgat ttccttggtg gggaagtcga ctttgagggc gaggcggccg
      241 ttgaggaccg tgccttcagt gggggtgtag ccgtcgggga cggcgatgaa gagggcgccg
      301 tcggcgccgc ggcggcgcga gaggacgagg tcgagcgtca ggttgtcggc atcccaggtt
      361 gcctgctgga tttccatggc gccggcggtc aggtgcatat cggtggcgat gacccacgga
      421 tgcgggcggc gagggcggaa gacgagcagg cgggtgtcgc gcgggggcac ggtgatctgg
      481 agggggccgg cgggttggcc gagatcctgg ctggtccaga attcatggac ccagcatggg
      541 ctgtgcgatt cggcggcgaa ctcggcggga tcgacggtga aggtccgttc ccgatcctcg
      601 gcgttgagca ggccgacgac atcccagcga tcccagtcgg tttcgaccgg caggtgccag
      661 acctggggat agccgccggg ataggggttg gtgaagagat cgaggggccg tgcggcgacg
      721 ccgtaggggg gcatggcggt ttcggcgatg cggaggcgct cctcggggac gtcgcgcagg
      781 tcctcggaga gattgaagat gccgccgcag aggccgacga gggcggtgcg gacgcgggcc
      841 tcgctgaggg tgccggggag accgacgcag agggcatcgg gctgggtgag ccagaggcgt
      901 cgatgggcgt accagcgcgc gccgatgcaa ctgatggcgg agacaaagcc ttcgtcgagg
      961 tgggagccgg ggccgccgat atcggcggcg cagttgacgc agtgcgcgag gccgacgaac
     1021 gacatggtgg gggcggaaca gtagtagacg aagtcggagg ggtcgagctc gagcgcctgg
     1081 gcctgggcgg tggcgcggag ggcttcgttg ccggggaccc tggtcttgtc gtggaagacg
     1141 cggtcggagc tgtaggggcc aaagaagtcg aagaggaagt atttgacgcc ttcgctcttg
     1201 aggcgtcgga tcgtctggcg gaggaagtcc tggacctcgg ggtgggtgcc gtcgaggaaa
     1261 tagacggtgc cgtcatgttt gaccctgggg ttgacctcgc gctgccagat gccgagccat
     1321 gaggaagaga gctggccgtc cttctcgcgc aggagccagt cgggatgctc gcggaagagt
     1381 gggacgtcgt cggtgacgag gaggaaggcg gcgaagatgc cgagcttcat gccgcgcttg
     1441 tcgagttcat cggcgagcca tttcatgccg ttgggatttt tctcggcatg ggcgacccag
     1501 ttgcccatgg agttgtcggc ctgccagcca tgtccccagt ggcagtattc gaaagggtaa
     1561 tcggcgaagc gctgcttgac gaaatcggcg ttgcgcagga cgaggtcggc gccgccctgg
     1621 aggcgggtgg cgtaccagct catgaagccc atggggatat agggccagat caggggctgg
     1681 ttgcgggcct tgacctcgtc gccgtagcgt tcgaggaggc ggaggggatc gcggtcggag
     1741 gcgatccaga ggggttcggt gtggacggtt tcgccgggtt ggattgcgag gccggcgaag
     1801 tgggagcggg cgtcgaggcg cagggggccg tcgtcgccga tggcgaggcg gatattggtg
     1861 agggcgcggt tccaggagag ggcgcagcag gcgagggcgg cctggcgcgc gccgtcgtag
     1921 aggacgctga cgccgcccca ggattcatgg atgcgtccgg tgcggatgct gaaggagacc
     1981 atgccctggc cttcctcggg ggtgtaggtc agggggctga cgaaacagtc gaggcgcttg
     2041 acgccggtcc aggtgctgcc gttgttgtcg gtgtagatcg tgaaaggtcg ccaatcgccc
     2101 tgatagtgga tgcggctgtt gccctggccg ttgacatcga gggcgtggac ggcttcgagg
     2161 gcgagggggc gatcgccgac gttggtgagg gaggttgcga tgaggagcga ctggccggct
     2221 tcgagggttt ggacattttg atcgaagcgc aggccgaggt cgggatcaag ccattggatt
     2281 ttccagaggg tttcggggcc ggcggtggat tggtcgatgc tggggggggc gagggtgcgg
     2341 gtgtcgctga ggcggccgtc ggccagttcg gcacggggca tggaatggct gaagccccaa
     2401 tcggggagga tgggggatag ataggtcaaa gcgccggagt tggcgtcgat gctcatggtg
     2461 gagcgctcct gatcgaatgg cgagatcgag tcacgcggac ggcgtcacgg agtgaacgat
     2521 tccgcgggcg gctgaatcgg cgatccgtca agcgagggcg gcggcgcgcc agcgagagtg
     2581 accggggggt cagcgccggc cctcggcggc cgcgggccag aactcatttg acgggcaact
     2641 atagggccgg ggggaagggg gctcaacccg cccattgtcg aaacgttgac ggcaattgac
     2701 gcggcgaggg gggaggggag ggcaggcaag ggggagaaag gacgaggagc ggagcacggg
     2761 agttgacggt cgaaatccga aacaatttcg aaagatgaaa ttcgagggaa ggagataagg
     2821 ggtgaggcgt tgatgacgtc gtgatcggcg ggatagcggg agagtggtat tcgagagccg
     2881 aggacgaggg acgagagccg agggccgaat tccggggcgg gccgttgggg gcgatgatct
     2941 cacgccgaga cgccaagacg cggaggggcg ccggcgtgga ggatgggtct gggacgggat
     3001 gagtcgggct tgaacgagga gggaacaatc attgaattgc gcgtcggggc cggccatcct
     3061 ctggcaccgc tggcaggata cgggggccgt agggaggcga ggccgaggac gcgggacgaa
     3121 ttgtggcggg gggtcagtcg gggacgacga cgggttggtc gcgctggatg attttatgcg
     3181 gttcggtcgg cgcgagggtc gggaggccct cggcggcggg gtcgttgacc tcggggatcg
     3241 cgtcgggtgg ggg
//
LOCUS       DAOPCO010000383         3240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23671_length_3240_cov_4.171115, whole genome shotgun sequence.
ACCESSION   DAOPCO010000383 DAOPCO010000000
VERSION     DAOPCO010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23671_length_3240_cov_4.171115"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1144
                     /gene="ribD"
                     /locus_tag="PLS90_16670"
     CDS             68..1144
                     /gene="ribD"
                     /locus_tag="PLS90_16670"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPK04080.1"
                     /translation="MALTLAARGRGKTSPNPMVGAVVVNGGKVVGRGYHARAGESHAE
                     VLAIREAGDLNHGATLYVTLEPCCHHGRTPPCVAAIQQAGLARVVAAMEDPNPLVRGR
                     GIEELRKAGIQVDVGCLKGEARRLNEFFVTFHEQRRPFVTIKWAMSLCGRTAHDSRES
                     RWITNQQSREHGHRLRSQHDGVLVGIGTVLSDDPMLNVRLANYHGPQPKRIVIDGDLS
                     IPIRARLLREREKGEVILFTTPFAKREMVDYFEQEGCRVIVVPSKRRLINMRLVLDEL
                     ARMGLMSVLIEGGRQIHTSLLTMGLVDKVVAFIAPKIIGGMQLRAPVEDLGLPRLENA
                     LVLRQVKFQTFGDDVYVEGYLREI"
     gene            1148..2017
                     /locus_tag="PLS90_16675"
     CDS             1148..2017
                     /locus_tag="PLS90_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04081.1"
                     /translation="MPLGQLSHLPPDLASNPFHPFLTRFARFKTGSFEVLFAVEAFLL
                     RYSWFALLVLFALVLPPLEEVDLLSTLILFLTLVYPCFFIWSLTRLAGVELMQCVLEG
                     HWTAELLATPIDNRDLTLGFLTPIWLIVRQYGLISVFSLFLYTLETQPVIIDGELYAA
                     DMAPPMILSYGLFFSAAAWIVFIYVARMFAEVRLRSGLLKGLWTLMLLLGGLALFALY
                     AAMLLLLPAETQSAPFLTFLMLLTAALAALSTWFYRKLVRLFREYLAGQLDIDLVIFD
                     DIDPRATGWTSEG"
     gene            complement(2177..>2824)
                     /locus_tag="PLS90_16680"
     CDS             complement(2177..>2824)
                     /locus_tag="PLS90_16680"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPK04082.1"
                     /translation="VLGEVAAPGPVALEGPIDLWRLIGRAGGFGPRADRRHVLVSRSA
                     GDGEERYLLDFDAWRFGIAPQENLAIEPGDLVYVRPAGGDHLWVLGDVPRPGRIALES
                     TEPLSLAQALGLAGLAPESRRQILVLRTRVDAGSIASSGSGRLQPLIFVVTDTAAERP
                     YGIDPSLQPEDIVFVPERPIEDLQGFAGQWFRDGRWQLMPWWRAMNGPAATSGIE"
     assembly_gap    2825..2834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2835..>3240)
                     /locus_tag="PLS90_16685"
     CDS             complement(<2835..>3240)
                     /locus_tag="PLS90_16685"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPK04083.1"
                     /translation="TPEYDQTARVRPDGRISLPLVGDVEAAGRTPDALRESLRERFAD
                     FLSEPELSVQILDAGGPAESLRASIAGALNGQSRLCRVRPDGYLSLPVLGDVAAAGGT
                     LDALRPEIEAAYRVRGLGAVEVSLALAEPPATE"
BASE COUNT          490 a          997 c         1178 g          565 t
ORIGIN      
        1 ctggcgagac gggcggtatg ggaactccgt gacgacattc aatcgcagcg accaatccta
       61 catgcagatg gcgctgaccc tcgcggcgcg ggggcggggg aagacgtcgc ccaacccgat
      121 ggtcggcgcg gtggtggtca acggcgggaa ggtcgtgggc cggggctacc atgcgcgcgc
      181 gggggaatcg cacgccgaag tgctggcgat cagggaagcc ggggatctga accacggcgc
      241 cacgctgtac gtgacgttgg agccgtgctg ccatcacggc cgcacccccc cgtgcgtggc
      301 ggcgatccag caggcggggc tggcgcgggt cgtcgcggcg atggaggacc ccaacccgct
      361 ggtgcgcggg cgcggcatcg aggaattgcg caaggcgggg attcaggtgg acgtcggctg
      421 cctgaaaggg gaggcacgcc ggctgaacga gtttttcgtg acattccatg agcaacgccg
      481 tccgttcgtc acgatcaagt gggcgatgtc gctgtgcggc cggacggcgc acgacagtcg
      541 cgaatcgcgc tggatcacga accagcaatc gcgcgagcac ggccatcggc tccgctcgca
      601 gcacgacggc gtgctggtgg gcatcggcac ggtgttgagc gacgacccaa tgctcaatgt
      661 gcggctggcg aactatcacg ggccgcagcc gaagcggatc gtgatcgacg gcgacctgtc
      721 gatcccgatc cgcgcgcggc tgctgcgcga gcgggagaag ggggaagtaa tcctgttcac
      781 cacgccgttc gccaagcgcg agatggtgga ctacttcgag caggagggct gccgggtgat
      841 cgtcgtcccg agcaagcggc ggctgatcaa catgcggctg gtgctggacg aactggcgcg
      901 gatggggctg atgtcggtgt tgatcgaagg agggcggcag attcacacct cgttgctcac
      961 aatggggctg gtggacaagg tggtcgcgtt catcgcgccg aagatcatcg gcgggatgca
     1021 actgcgggcg ccggtggagg acctggggct gccgcggctg gaaaacgcgc tggtgctgcg
     1081 ccaggtgaaa ttccagacgt tcggcgacga cgtgtatgtt gaaggctacc tgcgggagat
     1141 ctgacacatg ccgctgggac agctttcgca tctgcccccc gacctggcca gcaatccgtt
     1201 tcatccgttc ctgacgcgct tcgcccggtt caagaccggg tcgttcgagg tgctgttcgc
     1261 cgtcgaagcg tttctgctgc ggtattcctg gttcgcgctg ctcgtcctgt tcgcgctggt
     1321 gctgccgccg ctggaggaag tcgatctcct ctccacgctg attctttttc tgacactggt
     1381 ctacccgtgc tttttcatct ggtcgttgac gcggctggcc ggggtggagc tgatgcaatg
     1441 cgtgctggaa ggccactgga cggccgagct gctcgccacg ccgattgata accgcgatct
     1501 gacactgggg tttctgacgc cgatctggct gattgttcgg cagtacgggc tgatctcggt
     1561 cttctcgctg tttctctata cgctggagac gcagccggtc attatcgacg gcgagctgta
     1621 tgccgccgac atggcgccgc cgatgatcct gtcctacggg ctgttcttca gcgcggcggc
     1681 gtggatcgtg ttcatttacg tggcgcggat gttcgccgag gtccgcctgc ggagcgggct
     1741 gctgaagggg ctgtggacgc tgatgctgct gctggggggg ctggcgctgt tcgcgctgta
     1801 tgcggcgatg ctgctgctgc ttccggcgga gacgcaatcg gcgccgtttc tgacgttcct
     1861 gatgctgctg acggccgcgc tcgccgcgct gtcgacgtgg ttttatcgca agctggtgcg
     1921 gctgtttcgc gagtatctgg ccggccagct cgacatcgac ctggtgattt tcgacgacat
     1981 tgatccgcgc gcgacggggt ggacgtcaga ggggtgattg cttgcgccga ttcgcccccc
     2041 tttggggacg ggggatctcc cgcgtgacca tctccaggtt ccgctgcgct tcacctggga
     2101 ctattcaact gccgccccgc cggagcggtt cacgcggatg gatattgagc tttcctcaat
     2161 gttgatcagt tgacggctac tcgattccgg atgtcgcggc cgggccattc attgcgcgcc
     2221 accacggcat caactgccag cggccgtcgc ggaaccattg gccggcgaag ccctgcaaat
     2281 cctcgatcgg cctctcaggg acgaagacga tatcctcggg ctggagcgac ggatcgatcc
     2341 cataggggcg ctcggcggcc gtgtcggtga cgacgaagat cagcggctgc agtcgccccg
     2401 aaccggacga tgcgatcgag ccggcatcga cgcgcgtgcg caggaccagg atctggcgcc
     2461 gggattcggg cgcgaggccc gccaggccca gggcctgggc gagcgagagg ggttcggtcg
     2521 attcgagcgc gatgcgtccc gggcggggga cgtcgcccag cacccagagg tgatcgcccc
     2581 ccgccgggcg gacgtaaacc agatcgccgg gctcgatcgc gaggttttcc tgcggggcga
     2641 tgccgaagcg ccaggcgtcg aagtcgagca ggtagcgctc ctcgccgtcg ccggccgagc
     2701 gcgagacgag gacgtgtcgc cggtcggcgc gcggcccgaa tccgccggcg cggccgatca
     2761 ggcgccacag gtcgatcggg ccttcgagcg cgacgggacc gggcgccgcc acttcgccga
     2821 gcacnnnnnn nnnncctcgg tcgcgggcgg ttcggccagc gcaagggaga cctcgaccgc
     2881 acccagtccc cggacccgat aggccgcctc aatctcgggg cgcaaggcat cgagggtgcc
     2941 gccggcggcg gcgacgtcgc ccagcacggg gagcgacagg tagccgtcgg gacggacgcg
     3001 gcagagccgg gactggccgt tgagggcgcc ggcgatcgag gcgcgcaggc tttcggcggg
     3061 gccgcccgca tcgagaatct gcacgctcag ctccggctcg ctgaggaagt cggcgaagcg
     3121 ctcgcgcaag ctttcgcgca gggcgtcggg ggttcggccc gccgcttcga cgtcgccgac
     3181 gagcggcagg ctgatccggc catcggggcg gacgcgcgcg gtctggtcgt actccggggt
//
LOCUS       DAOPCO010000384         3237 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23705_length_3237_cov_4.608737, whole genome shotgun sequence.
ACCESSION   DAOPCO010000384 DAOPCO010000000
VERSION     DAOPCO010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23705_length_3237_cov_4.608737"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="PLS90_16690"
     CDS             <1..1775
                     /locus_tag="PLS90_16690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPK04084.1"
                     /translation="ARVYYRPIPRDTVELDERSPDSRFHPSVIVDSERPPFVETDANG
                     RFKIEHLRPGRYQLLAVAAGYLPASEIVAVEQGHTTEASFALRPRPAGQGDLAGIVVD
                     AQTHDPLRGAHVWVIPAGNVVIAEAMIPAVILGEAITDNTGRYRIEDLPTGPVIVFAA
                     REGYRTARKRVEIPAGGEAVARFALQPIPTGPTGHLAGTVLDARTEQPLEGVLVALIP
                     ARPTDFSLSRTPDSPIFRVTDENGQFAFRNLPVGHYRVRATREDYLPAVQLAEVLEGE
                     VTRLEFLLELQPLQLHGALAGVVTDAATSAPIEGAWVRLAGDEHAWLSNTERLVTRTN
                     AIGEYQFEHVPVGVYKVLVVKRGYEPGEGEAAILADQTTELNFALTPVEHAGAVEGVV
                     TDAVTGAPIAQAIVFIPIVAEPLSANEDNTLWAQTDEDGRYRIEDVPAGQRTAVCFAG
                     GYFPDAQIVTVVMDATTTLDFALVPWPPEGERNVRFRVHDRDGKRLAGVRIHVVLNDW
                     IQPGSSWDAHWSFTNADGVATIHGVPAGNWSIVATLEGYEPRLTPLTPVDETDTPAGS
                     DQLVELQFDLQNSNNAAGNWQAYP"
     assembly_gap    2098..2197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2198..2461)
                     /locus_tag="PLS90_16695"
     CDS             complement(<2198..2461)
                     /locus_tag="PLS90_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04085.1"
                     /translation="MKKGEGGGENREERRKGGGKRREEEREVVGVRMARIREPRTRDE
                     SREPSFGATDGSEGELIMNRRVISRIKMVGFGEPRTRDESREPN"
     gene            2561..3157
                     /gene="hisB"
                     /locus_tag="PLS90_16700"
     CDS             2561..3157
                     /gene="hisB"
                     /locus_tag="PLS90_16700"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPK04086.1"
                     /translation="MGASERIADIERKTNETDIRIKLNLDGKGTFQGTVGIGFVDHML
                     ELFARHGRLDLEVAGKGDLHIDQHHLVEDLGITLGQALARAAGDKRGIARFGQAAVPM
                     EETLVDCVLDFCGRPFLAFNVEIGPAKVGDFDTELTEDFFRALAMHAGLTLHLNLKYG
                     RNAHHVIEALFKATARALALAVRIDPGVDGVLSTKGKL"
BASE COUNT          512 a         1269 c          844 g          512 t
ORIGIN      
        1 gcgcccgcgt gtactaccgc cccatcccgc gcgacaccgt ggaactggac gagcgctcgc
       61 ccgactccag gttccacccc tcggtgatcg tcgacagcga gcggcccccc ttcgtcgaga
      121 ccgatgccaa cggccgcttc aagatcgagc acctgcggcc cggccgctac cagttgctcg
      181 ccgtcgccgc gggctacctg ccggcgagcg agatcgtcgc cgtcgagcag ggccacacga
      241 ccgaagccag cttcgccctc cgcccccgcc ccgccggtca gggcgatctc gccggcatcg
      301 tcgtcgatgc ccagacgcat gacccgctgc gcggcgccca cgtctgggtc attccggccg
      361 gcaacgtcgt catcgccgaa gcaatgatcc ccgccgtcat cctcggcgag gcgatcaccg
      421 acaacaccgg ccgctaccgg atcgaggacc tgcccaccgg tccggtgatc gtcttcgcgg
      481 ccagggaagg ctaccgcacc gcgcgcaaac gcgtcgaaat ccccgccggc ggcgaggcgg
      541 tcgcccgctt tgccctccag ccgatcccga ccggaccgac cggccacctg gcggggaccg
      601 tcctcgacgc ccgcactgaa cagccgctgg agggcgtcct ggtggccctg atccccgccc
      661 ggccgaccga cttctccctc tcgcgcacgc cggactcacc catcttccgc gtcaccgatg
      721 agaacggcca gttcgccttc cgcaacctcc ccgtcggcca ttaccgcgtg cgggcaacca
      781 gggaggatta cctccccgcc gtccaactgg ccgaggtgct cgagggcgaa gtgacgcgcc
      841 tggaattcct gctcgagctc caaccgctcc agctccacgg cgcactcgcc ggcgtcgtca
      901 ccgacgcggc cacctccgcg ccgattgaag gcgcctgggt ccgcctggcc ggcgacgagc
      961 acgcctggct gagcaacacc gagcgcctcg tgacccgcac caacgccatc ggcgaatacc
     1021 agttcgagca cgtccctgtc ggcgtctaca aggtcctcgt cgtcaagcgc ggctacgagc
     1081 cgggcgaggg cgaagccgca atcctcgccg accagaccac cgagctcaac ttcgccctga
     1141 ccccggtcga acacgccggc gccgtcgagg gcgtcgtgac cgacgccgtc acgggcgcgc
     1201 ccatcgctca ggccatcgtc ttcatcccga tcgtcgccga gcccctctcc gccaatgagg
     1261 acaatacgct ctgggcgcaa acggatgagg acggccgcta ccggatcgag gatgtgccgg
     1321 ccggccagcg gaccgccgtc tgcttcgccg gcggctattt ccccgacgcc cagatcgtga
     1381 cggtcgtcat ggacgccacg accacgctcg acttcgcgct cgtgccgtgg ccgcccgaag
     1441 gcgagcgcaa cgtgcgcttc cgcgtccacg accgcgacgg caaacgcctg gccggcgtcc
     1501 ggatccacgt cgtcctcaat gactggatcc agcccggttc aagttgggac gctcactgga
     1561 gcttcaccaa cgccgacggc gtggccacca tccacggcgt cccggccggc aattggtcga
     1621 tcgttgccac gctcgaaggc tatgagccac gcctgacgcc gctgacgccg gtcgacgaga
     1681 ccgatacccc cgccggcagc gatcagctcg tcgagttgca gttcgacctg cagaacagca
     1741 acaacgccgc cggcaactgg caggcctatc cctgacgcaa acgcgaagag acggctggaa
     1801 cgacaagacg cggccccacc cccggggggc cgcgtctttg ccatcagcca ccgatctcct
     1861 gcccatccct aaccttcgtt cctcgccctc gcccccatcg acccgcccca aaattcggct
     1921 ctcgactctc gtccctcgtc ctcggctctc caaaaccgac catcttattc gcgatatgac
     1981 ccgccgattc atgatcaact cgccctcgcc cccaacgacc cgccccaaga ttcggctctc
     2041 ggctctcgtc cctcgtcctc ggctctccaa aaccgaccat cttattcgcg atatgacnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatt cggctctcgg ctctcgtccc
     2221 tcgtcctcgg ctctccaaaa ccgaccatct taattcgcga tatgacccgc cgattcatga
     2281 tcaactcgcc ctcgctccca tcggttgccc caaaactcgg ctctcggctc tcgtccctcg
     2341 tcctcggctc tcgaatccgg gccatcctca cgccaaccac ctctctctcc tcctctctcc
     2401 tctttcctcc tcctttcctc ctctcctccc tgttctcccc ccctccttcc ccctttttca
     2461 ttggttcatc cgcccccttc tggcatgttg ggggaaactg acgcgcgccc gcggtttgcc
     2521 ctccgcaatc cgcccgagcg cgaccataga ggggacggcc atgggcgcca gcgagcggat
     2581 cgccgacatc gagcgcaaaa ccaacgaaac cgatatccgg atcaaactca atctggatgg
     2641 caagggcacg ttccagggga ccgtcggcat cggcttcgtc gatcacatgc tcgagctctt
     2701 cgcccgccat ggccgcctcg acctggaggt ggcgggcaag ggcgacctgc acatcgacca
     2761 gcaccacctg gtcgaggacc tcggcatcac gctcggccag gccctcgccc gcgccgccgg
     2821 cgacaagcgc ggcatcgccc gcttcggcca ggccgccgtt cccatggagg agaccctcgt
     2881 cgactgcgtc ctcgatttct gcggtcggcc gttcctcgcc ttcaacgtcg agatcggccc
     2941 cgccaaagtc ggcgacttcg acaccgaact gaccgaggac ttcttccgcg ccctcgccat
     3001 gcacgccggc ctgacgctcc acctcaacct caaatacgga cgcaacgccc accacgtcat
     3061 cgaggcgctg ttcaaggcca ccgcccgcgc cctggccctc gccgtccgca tcgatcccgg
     3121 cgtcgacggc gtcctctcga ccaagggcaa actgtaacgt gcgcgaacac gattcagggc
     3181 tccccgtgtc cctgatttcc ctgaagtccc cttccttcca tgccgcttga ccccaca
//
LOCUS       DAOPCO010000385         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23811_length_3225_cov_5.127129, whole genome shotgun sequence.
ACCESSION   DAOPCO010000385 DAOPCO010000000
VERSION     DAOPCO010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23811_length_3225_cov_5.127129"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1810)
                     /locus_tag="PLS90_16705"
     CDS             complement(20..1810)
                     /locus_tag="PLS90_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04087.1"
                     /translation="MRSRSNLFVVLMCFLLAGVCPAALVHESDFDEAPGTNPISAGDV
                     TAAWAIDGQFHGIYGWYDYDGDGRFILHHPNHNWDSPWFEIETSGTLDLDNDMELVVN
                     LYPKFDSAGDNQYMEIMLTEAGGDRPNLDCAFQLWQEYNELWVGFGPDGLSYGDSLDL
                     TNDELSSFTAIITYDDSAQVWHAFYRLNGGPLIEAPGSPLFGNRDYTTVKVWAEMDFS
                     PAADELEYELWFEDLSIYDEVSTFPPPGVVKESTFSSTDIFGNPVTNGEFTAMWFRDG
                     TCQPCGGPWAAYSTTGSLKMRMHRFNWDFPWAEYNTAGIIPTDNDMQVEWDFYPHFTS
                     GGSKQIVRFQISDPAPDNTTQPPVDRELVIENTGNNAWAIFPTGSPLYLGKPADEIQV
                     MKVIQTYEDATGQFRGFVSVDNGPLREVPPIAPLDRTKTVVRLKLDQGFPTSNTDYQI
                     HLNNMKISNVITVTEGSQIPIGTPMRNSAFDYFNNGAFVPINDPRDVGGLFFPGANTQ
                     AYNGTGQWVITKSTDADFPNATIPTVTTADQSAEVTVRVKPGWTADGVGQNFNFKWLG
                     NPGSVFCSNTNDVLTIMFGASSSARCVVGR"
     gene            complement(2007..3086)
                     /locus_tag="PLS90_16710"
     CDS             complement(2007..3086)
                     /locus_tag="PLS90_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HPK04088.1"
                     /translation="MAFDGLNLHLGNLSRLSDAESRSLSAENPTGARGQGGMAPEENN
                     PASKGLGQGWKCRPCVSVPPGETLEIASIDGPGAIQQMWFTINLHWRHAILRIYWDGQ
                     QQPSVECPMGDFFCSGWGRYAQVSSLAVCVNPGSAFNCYWEMPFRKHCRITVENLNDE
                     WLCLFYQINYTLTRVPDDAAYFHAQFRRTNPVPYKQVHTILDGVRGRGHYVGTYMAWG
                     VNNNGWWGEGEVKFYLDGDGEFPTICGTGTEDYFCGSYNFENQATHQYQEFTTPYAGM
                     PQVIRPDGLYSSQQRFSLYRWHVTDPIRFKQDLRVTVQALGWRNGCKRYLPLRDDLAS
                     VAFWYQTLPTAPFPPLPDKDALEVI"
BASE COUNT          571 a          991 c         1037 g          626 t
ORIGIN      
        1 gccctcccag caaccccgat cacctcccca cgacacatct tgcggaactg ctggcgccga
       61 acatgatcgt cagcacgtcg ttggtgttgc tgcagaagac gctgcccggg ttgcccagcc
      121 acttgaagtt gaagttctgg cccacgccat cggccgtcca gcccggcttg acccggaccg
      181 tgacttcggc cgactgatcc gccgtcgtca ccgtcgggat cgttgcgttg gggaagtcgg
      241 cgtcggtgct cttggtgatc acccactgcc ccgtgccatt gtatgcctgg gtgttggcgc
      301 cggggaagaa cagcccgccg acgtcgcgcg ggtcgttgat cggcacgaag gcgccgttgt
      361 tgaagtaatc gaaggccgag ttgcgcatcg gcgtgccgat cggaatctgc gagccttcgg
      421 tcacggtgat cacgttgctg atcttcatgt tgttcaggtg gatctggtaa tcggtgttgc
      481 tggtcgggaa gccctggtcc agtttcagcc ggacgaccgt tttggtgcgg tcgagcgggg
      541 cgatcggcgg gacctcacgc agcggaccgt tatccaccga gacgaatccg cggaactggc
      601 cggtggcgtc ctcgtaggtc tgaatgacct tcatcacctg gatctcgtcg gccggcttgc
      661 ccagatacag cggcgaccca gtcgggaaga ttgcccaggc attgttgccg gtgttctcga
      721 tcaccagttc cctgtcgacc ggcggttgcg tcgtgttgtc cggcgcggga tcgctgatct
      781 ggaaccgcac gatctgcttg gagccgccgc tcgtgaagtg cggatagaaa tcccactcca
      841 cctgcatgtc gttatcggtg gggatgatcc cggcggtgtt gtattccgcc caagggaaat
      901 cccagttgaa gcggtgcatg cgcatcttga gcgaaccggt ggttgaatag gccgcccacg
      961 ggccgccgca cggctggcag gtgccgtcgc ggaaccacat cgcggtgaat tcgccgttgg
     1021 tcaccggatt gccgaagata tcggtggaac tgaaggtgct ctccttgacg acgcccgggg
     1081 gcgggaaggt cgagacctcg tcataaatcg acagatcctc gaaccacagc tcatactcca
     1141 gttcgtcggc ggccggactg aagtccatct cggcccagac cttgaccgtc gtatagtcgc
     1201 ggttgccgaa gagcgggctg cccggggcct cgatcagcgg gccgccgttg agccggtaga
     1261 aggcgtgcca cacctgggcc gagtcgtcgt aggtgatgat ggcggtgaac gaggacaact
     1321 cgtcgttggt cagatcgagc gagtcgccat aggacaggcc gtcgggcccg aagccgaccc
     1381 acaactcgtt gtattcctgc cagagctgga aggcgcaatc caggttcggc cggtcgcccc
     1441 ccgcctccgt cagcatgatt tccatgtact ggttgtcgcc cgccgaatcg aatttcggat
     1501 agaggttcac caccagttcc atgtcgttgt cgaggtcgag cgtgcccgac gtttcgatct
     1561 caaaccaggg gctgtcccag ttgtgattgg gatggtgcag aatgaaccgg ccgtcgccgt
     1621 cgtagtcata ccagccgtag atgccgtgga actgaccgtc aattgcccag gccgccgtta
     1681 catcaccggc cgaaatggga tttgtgccgg gcgcttcgtc gaaatccgac tcatgtacca
     1741 gcgcggccgg acagacgccc gccagcaaaa agcacatgag gacgacgaaa agattggacc
     1801 gtgacctcat ggtcgcatcc tccgttaaag tttgaatcaa gtgcatcgag gttcccgtgg
     1861 cgccgtccgc ccggctgttc cgtcgcgccg ggccggtgca cggtgctgga gagcaatcgg
     1921 cgcggccccg ttccgctctc ctgccgattg gctcccgtct gttgactccg gatcacctcc
     1981 tctcccgtgg aatttccgtc gcaaagctag atcacttcca gcgcgtcctt atccggcagc
     2041 ggcgggaacg gcgccgtcgg cagcgtctga taccagaacg ccaccgacgc caggtcgtcg
     2101 cgcagcggca gataacgctt gcaaccattg cgccagccga gcgcctgaac cgtcacccgc
     2161 aggtcctgct tgaaccggat cggatcggtc acatgccagc ggtagagcga gaaacgctgc
     2221 tgcgagctgt agagcccgtc ggggcggatc acctggggca tgccggcgta gggggttgtg
     2281 aactcctggt actggtgcgt cgcctggttt tcgaaattgt aggagccgca gaagtagtcc
     2341 tcggttcccg tcccgcagat cgtcggaaac tcgccgtccc catccagata gaacttgacc
     2401 tcgccctcgc cccaccagcc gttgttgttg acgccccagg ccatgtaggt gccgacgtag
     2461 tggccgcggc cgcgcacgcc gtccagaatc gtgtgaacct gcttataggg gaccgggttg
     2521 gtgcgacgga actgggcgtg gaaatacgcg gcgtcgtcgg ggacgcgcgt cagcgtgtag
     2581 ttgatctggt agaacaggca cagccactcg tcgttgaggt tctccaccgt gatgcggcag
     2641 tgcttgcgga acggcatctc ccagtagcag ttgaaggcgc tgccggggtt gacgcagacg
     2701 gccagggagg agacctgcgc gtagcgcccc cagccgctgc agaaaaagtc gcccatcggg
     2761 cactcgaccg agggctgctg ctgaccgtcc cagtagatgc gcaggatagc gtggcgccag
     2821 tgcagattga tcgtgaacca catctgctgg atcgcgccgg gtccgtcgat gctggcgatc
     2881 tccagcgtct cgccgggggg aacgctgacg cacggccggc acttccagcc ctggcccagg
     2941 cccttgctgg ccggattgtt ttcctcgggg gccatgccgc cctggccgcg ggcgccggtc
     3001 ggattttccg cgctcaacga gcgcgactcg gcatcggaga gccggctcag gttgcccagg
     3061 tgaaggttca agccatcaaa cgccatgcga ccggggaatc ctttttgccg atgggcgcgg
     3121 tcgcgggagc ggaaacaacg gttggcgagc aacgcatcgg aaaatcgagt tcggccagag
     3181 tggaaaacga gaccttgatc tgaccatttg tcaccctatc aatct
//
LOCUS       DAOPCO010000386         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_23954_length_3209_cov_4.999683, whole genome shotgun sequence.
ACCESSION   DAOPCO010000386 DAOPCO010000000
VERSION     DAOPCO010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23954_length_3209_cov_4.999683"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2324)
                     /locus_tag="PLS90_16715"
     CDS             complement(<1..2324)
                     /locus_tag="PLS90_16715"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04089.1"
                     /translation="MSKPTFLLNQPWELTHFPQESIDAGEVLAGTAELQWIPASVPGS
                     VVLDMRRAGLVPDPFVGMNPEEIRPLEYHEWWYRTQFVAPAEFAAPGGRLDLVFEGID
                     TFADIYLDGELIGSAANMLIPHRIALEGLTPGAPHTLMVRIKSDQYEVSQKDFDGVVE
                     LVGNDPSRVWIRKAGHTHGWDIFPRLLSAGIWKPVYLERTPEYSIDDLFVFTRRWGRE
                     RCTILARVTLNCPRVYRTDMAVRFRLLDAGQEVYGCTYAVQFPLVKFEFPLENPKMWW
                     PRPYGEPHLYEAEAVLLQDGREIDRQTAPFGIRSIEIVMEDLPDDRQSFYLKINDEPV
                     SLYGTNHVPLDSFPSQQAERLERAIELLDDSRCNCVRVWGGGVYEPQAFYDACDRLGI
                     LVWQDFMFSCALYPQEESFLQQVREEAAWVVKALRNHPSVVLYCGDNEIETHLSAYFG
                     PHQQGGNRAIAHELLPEWVTRYDGTRPYWPSSPYTPVAGLPAHSIKAGDTHIWCHGLY
                     YKAPVYSQDTSPFISEIGHLAFPDLATMQSFMPKDKLWPYDRFVLGYRCGSLSDPTFF
                     TYPDGRLKAIEKNVTNVFGALPDDLADLIFATQLVQAEAFKSWIERARRRKFDCGGIL
                     WWNLLDGCPQCSDAVVDYYFRKKLAYNYIRRAQDDVLICMEECEGELRVFLVNDNLMP
                     LDLSYQVRCDDAVVMEGRCEIPANSVVDLGSVGAPSETARLYLLRAEISDRRYCNHYL
                     DGRPPFAFEELREYWKRIEEHAVRPRADEGDVAL"
     gene            complement(2389..3150)
                     /locus_tag="PLS90_16720"
     CDS             complement(2389..3150)
                     /locus_tag="PLS90_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPK04090.1"
                     /translation="MLFPLLWMISTSLKAPGKEYIMPPSLIPDPVHWRNYLEVFRAMP
                     FGRFLFNTCLVTVAVTFGQALTCSMAAFAFSRLKFPGRDKIFLLYLTTLMIPGVVTLI
                     PLFVIMQYFGWIDTYQALIIPGLFSAYGTFLLRQFFLGIPLALEDSAHIDGCGPWGVY
                     WNVILPLSKPALVTLIIFTFMGAWRDFLWPLVITNSLEMRTLTVGLSIFRGAYVSDWN
                     LMMAGSCLVMAPLVFVFLFNQRFFVEGIQLTGIKG"
BASE COUNT          636 a         1028 c         1026 g          519 t
ORIGIN      
        1 tgcagggcga catccccctc gtcggcccgc gggcggaccg cgtgctcctc gatccgcttc
       61 cagtattcgc gcagttcctc gaacgcgaac ggcggccgcc catccagata gtgattgcag
      121 tagcggcggt cgctgatttc ggcgcgcagc agatacagcc gcgccgtttc ggacggggcg
      181 cccacgctgc ccaggtccac aacgctgttc gccggaattt cgcaccggcc ttccatcacg
      241 acggcgtcgt cgcagcggac ctgatagctc aggtcgaggg gcatcaggtt gtcattgacc
      301 aggaagaccc gcagctcgcc ctcgcactcc tccatgcaga tcagcacgtc gtcctgcgcg
      361 cgccggatat agttgtaggc gagcttcttg cggaagtagt agtcgaccac cgcgtcggag
      421 cactgcgggc agccgtccag cagattccac caaaggatcc ccccgcaatc gaacttgcgc
      481 cgccgcgccc gctcaatcca cgacttgaac gcttcggcct gaacaagctg cgtggcgaag
      541 atcaggtcgg cgaggtcgtc gggcagcgcg ccgaagacgt tcgtcacatt cttctcgatc
      601 gccttcaagc gtccgtcggg atacgtgaag aacgtcgggt cgctcagcga accgcagcga
      661 taccccagca cgaagcggtc gtacggccag agcttgtcct tcggcatgaa gctctgcatc
      721 gtggccagat cggggaaggc caggtgcccg atctcgctga tgaacggcga cgtatcctgg
      781 gaataaaccg gcgccttgta atacagccca tggcaccaga tgtgggtgtc gccggccttg
      841 atcgagtggg ccggcaggcc cgcgaccggc gtgtagggcg acgagggcca gtacggccgc
      901 gtgccgtcgt agcgcgtcac ccattccggc aggagctcgt gcgcgatggc ccggttgccc
      961 ccttgctggt gcggcccgaa gtacgcgctg agatgcgtct cgatctcatt gtcgccgcag
     1021 tagagcacga cggagggatg attgcgcagc gctttgacga cccaggcggc ctcctcgcgc
     1081 acctgctgca ggaacgattc ctcctgcgga tagagcgcgc aggagaacat gaaatcctgc
     1141 cagacgagaa tcccgagccg gtcgcacgcg tcgtagaacg cctgcggctc gtagacgccg
     1201 ccgccccaca cgcgcacgca gttgcagcgg ctgtcgtcga gcagctcaat cgcccgctcc
     1261 agccgctccg cctgctgcga cgggaacgaa tcgagcggga cgtgattcgt cccgtagagc
     1321 gacaccggct cgtcattgat tttcaggtag aatgactggc ggtcatcggg cagatcttcc
     1381 atgacgatct cgatcgagcg gatgccgaac ggcgccgtct gccgatcaat ctcgcggccg
     1441 tcctggagca gaaccgcctc ggcctcgtac aggtgcggct cgccataggg gcgcggccac
     1501 cacattttcg ggttctccag cgggaactcg aacttgacca gcggaaattg cacggcgtag
     1561 gtgcagccat agacttcctg accggcatcg agcaggcgga agcggacggc catgtcggtt
     1621 cgatagaccc gcgggcaatt gagcgtgacg cgggccagga tcgtgcagcg ctcgcgcccc
     1681 caccggcgcg tgaagacgaa cagatcgtcg atcgagtact cgggagtccg ctcgaggtag
     1741 accggcttcc agatgccggc cgacaggagc cgcgggaaga tgtcccagcc gtgcgtgtgg
     1801 ccggctttgc ggatccagac gcggctcggg tcgttgccga ccagttccac gaccccgtcg
     1861 aagtccttct gtgacacctc atactgatcg ctcttgatcc gcaccatgag cgtgtgcggc
     1921 gcgcccggcg tcagaccctc gagcgcgatg cggtgcggga tcagcatgtt ggcggcgctg
     1981 ccgatcagct cgccatcgag atagatgtcg gcgaatgtgt cgatcccctc gaaaacgaga
     2041 tcaagccggc cgccgggagc ggcgaactcc gccggcgcaa cgaactgcgt ccggtaccac
     2101 cattcgtgat actccagcgg gcgaatctcc tccgggttca tgccgacgaa cggatcggga
     2161 accagtcccg cccgccgcat atccagcacc acgctgccgg gcacgctggc cgggatccac
     2221 tggagctcgg ccgtgccggc aaggacctca ccggcgtcaa tggattcctg cgggaagtgc
     2281 gtcagctccc atggctgatt gagtagaaag gtaggcttgg acatcgatcg ttccttcggt
     2341 cgtcggcgtg atttgccgcg gccctgaagg gccgggccgg cgggcgggtc agcccttgat
     2401 ccccgtcaac tggatcccct ccacgaaaaa tctctggttg aacaggaaaa caaagaccag
     2461 cggcgccatc accaggcacg agccggccat catcaggttc cagtccgaga cgtaggcccc
     2521 ccggaaaatc gaaaggccga ccgtcagcgt ccgcatttcc agcgaattgg taatgaccag
     2581 cggccagagg aaatcgcgcc acgcgcccat gaacgtgaaa atgatcagcg tcaccagcgc
     2641 cggcttggag agcggcagga tcacgttcca gtagacgccc catggcccgc agccgtcgat
     2701 atgcgccgag tcctccaggg ccagcggaat gcccaggaaa aactggcgca gcaggaacgt
     2761 cccatacgcg gagaacaggc cgggaatgat cagtgcctga taggtatcaa tccagccgaa
     2821 atactgcatg atgacgaaca gcgggatgag cgtaacgacg cccggaatca tcagcgtcgt
     2881 caggtagagc aggaagatct tgtcgcgtcc ggggaacttg agccgggaaa aggcgaacgc
     2941 cgccatcgaa caggtcagcg cctggccgaa cgtgaccgcc accgtcacca ggcaggtgtt
     3001 gaacaggaac cggccgaacg gcatggcgcg gaagacttca aggtagttgc gccagtggac
     3061 gggatcgggg atcaggctgg gcggcatgat gtattccttc cccggcgcct tgagcgaggt
     3121 ggagatcatc cacagcaggg gaaaaagcat cgtgaacccg agcccccaga cgaacagcgt
     3181 gaagacgatg cggccgggcg tcagcccgc
//
LOCUS       DAOPCO010000387         3160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_24400_length_3160_cov_8.154267, whole genome shotgun sequence.
ACCESSION   DAOPCO010000387 DAOPCO010000000
VERSION     DAOPCO010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24400_length_3160_cov_8.154267"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            7..1176
                     /locus_tag="PLS90_16725"
     CDS             7..1176
                     /locus_tag="PLS90_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HPK04091.1"
                     /translation="MSIQDLVSRSLDEIRQNLFDRLTSKQEEYAATGWLPIRLNLNKG
                     IVRGLIELWAWGLWQLYQFLGEILKQAFPDTATGKWLELHCRQVGVSRKAWTKTQGVV
                     YFIRLAAGGNVAIPAGRVVKTKPDGAGNVYRYVTTAAVVLAAGTLEVAVPVEAEEFGA
                     ASNATAGQISEIATVIPGVDGVENRADWLTVEGTDEELDEPLRLRYQLAWQSLNGCTK
                     HAYQAWALEVTGVVDVKILDQHPRGQGTLDVLIVGSAGVPTQQLIDAVAANILGTGND
                     DEKNPINDDVEVRGPTPINIDYAAELELISGDEAAIVAEAENRVRALFAAGPVLAGIS
                     RLGVGQDVTQDRLNWAVMLPTVKKINTDFADVAVPEDGLAALHSINITATWAAEE"
     gene            1178..1819
                     /locus_tag="PLS90_16730"
     CDS             1178..1819
                     /locus_tag="PLS90_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04092.1"
                     /translation="MGRFWDYFRNELRFPPILRPGALALLADGGSETLDAVRDAQMQL
                     RDQFLPARCEDGYLVHFARSRGITRHPLEPVEHWSTRVRFAYHWWSRGGRACAMAEAL
                     QSGFNFAAVMVFNHGSEGSLIDETSGIQLVDETSGAILNYERDPSRWAEFSVTIYLAG
                     NEYAYTVDQVIWAINEIKPARSKLAELLWIAPLHDETSGAGLYDETTGTPLTS"
     gene            1846..3096
                     /locus_tag="PLS90_16735"
     CDS             1846..3096
                     /locus_tag="PLS90_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04093.1"
                     /translation="MAGESISWKTYLAARAPATPPLGDDVLTILQGGTDVKKLRWEDL
                     VRGVANYCEDTGAANALVIASATNYAAYFAGLPFLVKVASTNTGAATMNLNNLGAKSI
                     KKLGSAALDPGDLVAGQLVCLAYDGTYFQLLSPPVQGLRYAVATGADNSYAIDLAPAP
                     GAYIDGMQVIIKANHTITGPATLNINELGAIAIKKNGSAALRADDIRNGQVICVVYDG
                     ANFQLLSPPTSNLQYAVATGADNAYAIALTPAMDAYIEGMSIVFKANHDNAGAATLSV
                     DGMAAKAIKKNGSVALEAGDIKNGQIITVVYDGVNFQLINNGQVDMSQFGRNLGVTGY
                     QKLPGGLIIQWGKNELSGSTTQVISLPIAFQEDMYIGVCQSNTGGMTPGSAESAIYKT
                     SLSQISIQNCYTSILVIGWIVIGK"
BASE COUNT          694 a          823 c          977 g          666 t
ORIGIN      
        1 taagcgatgt caatccaaga tctggtctct aggtcgctgg acgaaatacg tcaaaacctg
       61 tttgaccgcc tgacgagcaa acaagaggaa tatgccgcca cgggatggct gccgatacga
      121 ctgaatctga ataagggaat cgttcgcggg ttgatcgagt tgtgggcgtg gggactctgg
      181 cagctctatc aattcctcgg ggagatcctg aagcaggcct ttcccgacac ggccacggga
      241 aaatggctgg aactgcactg ccgacaggtg ggcgtgagcc ggaaagcctg gaccaagacc
      301 cagggggtcg tctattttat ccgcttggcg gccggtggca atgtggccat tccggccggg
      361 cgcgtcgtca agaccaaacc ggacggagcc ggaaatgtct acagatatgt gacgaccgcc
      421 gccgtagtac tggctgccgg aacgctggaa gtggccgtgc ccgtcgaggc ggaggaattc
      481 ggcgccgcat ccaatgctac ggccggacag ataagtgaga tcgcaacagt gattcccggt
      541 gtcgacgggg tggaaaaccg cgccgattgg ctgaccgtgg agggaaccga tgaagagctc
      601 gatgagccgc tccggctccg ttatcaactg gcgtggcaat ccctgaacgg ctgcaccaag
      661 catgcctatc aggcgtgggc gctggaggtg acaggagtgg tcgatgtcaa gatcctcgat
      721 cagcatcctc gtggccaggg gactctggat gtgctcattg tcggctcagc aggcgtgccg
      781 acccagcaac tcatcgatgc ggtggccgcc aacatcctcg gcaccgggaa cgatgatgaa
      841 aaaaatccga tcaatgacga cgtagaggtg agagggccga ctcccatcaa tatagactat
      901 gcggcggagc tggaactgat ttccggcgac gaggcggcaa tcgttgccga agctgaaaac
      961 cgggtgcgtg cattattcgc ggccggtccc gtcttggccg gcatcagtcg cctgggcgtc
     1021 ggccaggatg tcacgcagga ccggctcaac tgggctgtca tgcttcccac ggtaaagaag
     1081 attaacactg attttgccga tgtggcagtt cccgaggacg ggctggcggc gctgcatagc
     1141 attaacatta ccgcgacatg ggcggctgag gagtagcatg gggcgtttct gggattattt
     1201 caggaatgaa ttacggtttc cgcctatcct gcggccgggt gcgttggcgc tcttggccga
     1261 tggggggagt gaaacgctcg atgctgttcg agatgcccag atgcaactgc gcgatcagtt
     1321 tctgccggcc cgatgtgaag atggctatct cgtccacttc gcgcggagcc gcggcatcac
     1381 tcgtcacccg ctggagccag tggagcattg gtcgaccagg gtgcggttcg cataccattg
     1441 gtggtcgcga ggcggccgcg cctgcgccat ggccgaggcg ttgcagtcgg ggtttaattt
     1501 tgcggccgtg atggtgttca atcatggctc cgaaggatcg ctgatcgatg aaacgagcgg
     1561 gattcaactg gttgatgaga cgtccggcgc gatattgaat tacgagcgcg atccgtcgag
     1621 gtgggcggaa ttctcagtta cgatttattt ggccgggaac gagtatgcct ataccgtgga
     1681 ccaggtcatt tgggctatca acgagattaa gccagctcgc tcgaagctgg cagagttatt
     1741 atggatcgca cctctgcatg atgaaacgag cggggccggg ttgtatgacg agacaacggg
     1801 cacgccgttg acatcgtaat tttcaatatg aagggagatt aaattatggc cggcgaatcg
     1861 atatcatgga aaacctatct agcggcacgt gctcccgcaa cccctccgct cggagatgat
     1921 gtgcttacga ttctccaggg ggggacggat gtgaaaaaac tccggtggga ggacctcgtg
     1981 cgcggggtgg ccaattactg cgaagacacg ggggctgcca atgcgctggt gattgcctcc
     2041 gcgacgaatt atgcggccta tttcgcgggg ctcccgttcc tggtgaaggt tgcgagcacc
     2101 aataccgggg ccgcaaccat gaacctcaac aacctgggag ccaagtccat caaaaaactc
     2161 ggatcggccg ccctggatcc gggggatctc gttgccggac aactcgtgtg tctcgcctat
     2221 gatggtacgt attttcagct cctttcgcct ccggtccagg gtctgcgtta cgcagtggcc
     2281 accggcgccg acaactccta tgcgattgac ctggcacctg cgccgggtgc ctacattgat
     2341 gggatgcagg tcattattaa ggcaaatcac accatcaccg gtcccgcgac gctcaatatc
     2401 aacgaactcg gggcgatcgc aattaagaag aacggctccg ccgccctgag ggcggatgac
     2461 atcaggaacg ggcaggtgat ctgtgtcgta tatgatgggg ccaacttcca gcttctttcc
     2521 cctccgacct cgaatctgca gtacgcggtc gccaccggtg cagacaacgc ctacgcgatt
     2581 gccctgaccc ccgccatgga tgcctatatc gaggggatgt cgatcgtctt taaggccaat
     2641 cacgacaatg ccggggcggc caccctgtcc gtagatggca tggcggcgaa ggcgatcaaa
     2701 aaaaacggaa gcgtcgccct ggaggctgga gatatcaaaa acggccagat catcactgtc
     2761 gtttacgatg gcgtgaattt tcagctcatc aataatggcc aagttgatat gagccagttc
     2821 ggcaggaatc tgggcgtcac ggggtatcag aaattgccgg gtggtctgat tattcagtgg
     2881 gggaagaacg aactttccgg ctcaactaca caggtcatct ctctccccat agcgtttcag
     2941 gaagacatgt atattggggt atgccagtcc aacacagggg ggatgacgcc tggcagtgcc
     3001 gaatcagcta tatacaagac gtcattgtcc caaataagca tccagaattg ctatacgtcc
     3061 atcctggtta ttgggtggat tgttataggg aaataaggag gagatatgct ttatttttct
     3121 gcgtcatctt cgggttttta tgatggggac atccacgggt
//
LOCUS       DAOPCO010000388         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_24489_length_3151_cov_5.397287, whole genome shotgun sequence.
ACCESSION   DAOPCO010000388 DAOPCO010000000
VERSION     DAOPCO010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24489_length_3151_cov_5.397287"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..890
                     /locus_tag="PLS90_16740"
     CDS             120..890
                     /locus_tag="PLS90_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04094.1"
                     /translation="MATLTEREEIERYQKLLEDWKPAPPSKKWKTQPVTSGPARPAPA
                     APAAPTNGQLLRQPGAILKLGDGPVVIYLRPVPQKEYHLVLALFPNGSIKTQGVALDA
                     YPVEELGAIPPVWLERMQQEMRWNRDLIVFHCYSYDDVRKLPDAMGAPADAPPAARTP
                     VPSPKAQPEAPAARPPDDPQPAPPRRGSRLQIRFGNNVWDAIYWGKDDQGHVVAHQTN
                     NQWALMHLDLDRFGNGVQVDPNPDLALVEQIEKSLLAQ"
     gene            complement(1141..1956)
                     /gene="truA"
                     /locus_tag="PLS90_16745"
     CDS             complement(1141..1956)
                     /gene="truA"
                     /locus_tag="PLS90_16745"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPK04095.1"
                     /translation="MHQKELTPSRRIRLIVQYDGAAFYGWQSQAGQRTVQDELEGALQ
                     RLLGLSRRVRVTAAGRTDTGVHALAMPVHFDLRHPIPTENLAVALTKFLPEEIGVLAA
                     AEAPPSFDARRRAILRWYRYQILRERLRRPLAPRGWRVTRPLDLERLAWGLEQLRGTH
                     DFRGFRSSQCQGKRTVLTMEQATLTEAGTLLALDFKCRSFLHHMIRFMVGSLAALAGG
                     RLSRERFLRILEQGERPQLAACAPPGGLCLMGVAYSEAEREELLARTPPPPSF"
     gene            2115..3095
                     /locus_tag="PLS90_16750"
     CDS             2115..3095
                     /locus_tag="PLS90_16750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPK04096.1"
                     /translation="MDRRRFLAALAAAGAVSGLSPSYLQAKEEAEMNPKGLQPVARRP
                     LGRTGEELSIIGFGGIIVMNQTPAESANYVAEAVDRGVNYFDVAPSYGNAQERLGPAL
                     KPHRDRCFLACKTGMRDAAGAEKELHESLRLLQTDHFDLYQLHGITDVEKDVNAAFAP
                     GGCMEPILKAREAGKIRFLGFSAHSEEAANAAMDQFDFDTILFPFNFAAWHKGQFGPS
                     IHQRAGERNMGRLALKAMAHQSVGNREKKWDKCWYIPIDETDTAALGLRFTLGLPVVA
                     AIPPGHWQLFDMALRLAQSGALDVPLNDQEQAAVQELARAADPLFSKSAA"
BASE COUNT          536 a         1152 c          936 g          527 t
ORIGIN      
        1 cgagccggtt tcgcgctccc cgcgcatcgt tgacgcttgt ccctttatgt catatcctgt
       61 tgttgtctgc ggttttttgc ccaccgcaca ccgataccca ttgaacagga cttccgtgag
      121 tggccacctt gactgaacgt gaagagattg aacgctacca gaagctcctg gaggactgga
      181 aaccggcgcc cccttccaag aaatggaaga cccagccggt cacctcgggc cccgctcgcc
      241 cggctccggc cgccccggcc gcgccgacga acggccagct cctccgtcaa cccggcgcga
      301 tcctgaagct gggcgatggc ccggtggtca tctacctgcg gcccgtgccg cagaaggagt
      361 accatctcgt cctggcgctc ttccccaacg ggtcgatcaa gacccagggg gtcgcgctcg
      421 atgcctaccc ggtggaagag cttggcgcaa ttcccccggt ctggctggag cggatgcaac
      481 aggagatgcg gtggaaccgc gatctgatcg tctttcactg ctacagttac gacgacgtcc
      541 gcaaactgcc cgacgcgatg ggcgctcctg cggacgcccc gccggccgcc cgcaccccgg
      601 tgccgagccc caaagctcaa cccgaggccc cggccgcgcg gcccccggac gatcctcagc
      661 ccgccccgcc gcgccgcggc agtcggctcc agatccgctt cggcaacaac gtctgggacg
      721 ccatctactg gggcaaggac gaccagggtc acgtcgtcgc gcatcagacc aacaaccaat
      781 gggcgctgat gcatctggat ctggaccgct tcggcaacgg cgttcaggtc gatcccaatc
      841 ccgatctggc cctcgtcgag cagatcgaga aaagcctcct ggcgcagtga gccggaccgc
      901 cgcttaaccc tcttggaacc ggagtcgcag taacccctaa cccgctttga agatgtgctg
      961 attggccgta ccaacgcact gatacatttc tcattcatcc cggaacgtcc ggccgccagg
     1021 ccggacgttc ttttttcctc gtctcggctt cgcgatcaat cctgtttttg ttggcattcc
     1081 agattcgaat ttgtttcgga ttttgaaatt cgcgtttcgt gtttcctatc cgatacttgt
     1141 tcaaaaactc ggcggcggcg gcgtccgcgc cagcagctct tcgcgctccg cttcgctgta
     1201 ggccacgccc atcaggcaca acccccccgg cggcgcgcac gccgccagtt gcggccgctc
     1261 gccctgctcc aggatccgca ggaatcgctc gcgcgacagc cgcccccccg ccaacgccgc
     1321 caggctcccc accatgaacc ggatcatgtg atgcagaaac gaccggcatt tgaagtcgag
     1381 ggcgagcaac gtccccgcct cggtcagcgt tgcctgctcc atcgtcaaca ccgtccgctt
     1441 cccctggcac tgactggatc gaaagcccct gaaatcatgc gtcccccgca actgctccag
     1501 cccccatgcc aaccgctcca ggtcaagcgg ccgggtcact cgccagccgc gcggcgccag
     1561 cggccggcgc agccgctccc gcaggatctg gtagcggtac cagcggagga tcgcccggcg
     1621 gcgggcatcg aacgagggcg gcgcctcggc cgccgccagc acgccgatct cctcgggcag
     1681 gaatttcgtc agcgcgacgg caaggttctc cgtcgggatc gggtggcgca aatcgaagtg
     1741 caccggcatc gccagcgcgt gaacgcccgt gtcggtccgc cccgcggccg tcacgcgcac
     1801 ccgccggctg agccccagca gccgctgcag cgccccctcc agctcatcct gcaccgtccg
     1861 ctgacccgcc tgcgactgcc agccgtaaaa cgccgcgccg tcatactgca cgatcagccg
     1921 gatccgccgg ctgggagtca gttctttctg atgcaccgcc cgcccgtccg tcgtagaatg
     1981 agcgccgtca tcaagcgtgc cggaatcgcg gtcggcgttg ggtccccggg ctcatgccga
     2041 gccgcgcggg ttcgcccccg gccgcgagtc ccgacgcgag cccactctac accgcccgcg
     2101 gaaaggtgtg catcatggat cgtcgtcgct ttctggccgc gctggccgcc gccggagccg
     2161 tctccggcct ctcgcccagc tatcttcagg caaaggagga ggccgaaatg aatcccaagg
     2221 ggctccagcc cgtcgcgcgc cggccgctcg gccggaccgg cgaggaactg tcgatcatcg
     2281 gtttcggcgg catcatcgtc atgaaccaga cccccgcgga gtcggccaat tatgtcgccg
     2341 aagccgtcga ccgcggcgtc aactacttcg atgtcgcccc cagttacggc aatgcgcagg
     2401 agcgcctcgg cccggcgctc aagccgcatc gcgaccgctg cttcctggcc tgcaagaccg
     2461 ggatgcgcga cgccgccggc gcggagaagg aactgcacga atcgctccgc ctgcttcaga
     2521 ccgatcattt cgatctgtat cagcttcatg ggatcaccga cgtggagaag gacgtcaacg
     2581 ccgccttcgc tccgggcggg tgcatggaac cgatcctcaa ggcgcgcgag gccggcaaga
     2641 tccgcttcct cggcttctcc gcccattccg aagaagcggc caacgccgcc atggatcagt
     2701 tcgatttcga cacgatcctc ttcccgttca atttcgccgc ctggcacaag gggcagtttg
     2761 gcccgagcat ccaccagcgc gccggcgaac gcaacatggg ccgcctcgcg ctcaaggcca
     2821 tggcgcatca gtcggtcggc aaccgcgaaa agaagtggga caagtgctgg tatattccga
     2881 tcgatgagac cgacaccgcc gcgctcggcc tgcgcttcac gctggggctg cccgtcgtgg
     2941 ccgccatccc gccggggcac tggcagctct tcgacatggc gctgaggctg gcccagagcg
     3001 gcgcgctcga cgtcccgctc aacgatcagg aacaggcggc ggttcaggaa ctggcgcgcg
     3061 ccgccgatcc gctgttcagc aaatccgccg cctgagaccg gccgcgaaaa gagttgtcat
     3121 ggctgacctg tggcccgagc ggatcgacgc c
//
LOCUS       DAOPCO010000389         3131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_24667_length_3131_cov_3.700910, whole genome shotgun sequence.
ACCESSION   DAOPCO010000389 DAOPCO010000000
VERSION     DAOPCO010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24667_length_3131_cov_3.700910"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..1115)
                     /locus_tag="PLS90_16755"
     CDS             complement(258..1115)
                     /locus_tag="PLS90_16755"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPK04097.1"
                     /translation="MPLAILIGFALVFSVCIFLLQNRMIYYPSRYDSAWLDRMRGAIT
                     FLPFETSQGSQQAYYVPPRAPAAHAAPPVRLWVLFHGNAARALDWLDTIEPLPDAGAA
                     YLLVDFPGYGASAGKPTPGTILEASQGALDALARDLNLDRAALNDRLHLMGYSLGAAA
                     ALQLARHTGARRIVLLAPFSDMLAMARRTVGWPLACLLRHRFDNRAALAALAERPERP
                     AVTILHGDADPLVPVEMSRQMAREHDWIELIELPRADHDWLLDMAGDRLPAAMGMTAA
                     PPISPSLAP"
     gene            1583..2053
                     /locus_tag="PLS90_16760"
     CDS             1583..2053
                     /locus_tag="PLS90_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPK04098.1"
                     /translation="MSKSYATADGAAGQIVLRDVSLRVAGGEFLALMGSSGSGKTTLL
                     NLIGGLDTPDSGRIHVGSQDLQRMSDNARSAFRLRHIGFVFQFFNLLPYLSVRENIAL
                     PLLFLRRPVPEAEDRSSALAAEVGLADKLDRQSNQLSGGEMQRVALARALVHEW"
     gene            complement(2396..>3131)
                     /locus_tag="PLS90_16765"
     CDS             complement(2396..>3131)
                     /locus_tag="PLS90_16765"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HPK04099.1"
                     /translation="GEWIVPADSGSAFVYRHLDDSWVLEAELNSGLEAQQNEFLGYSV
                     AVSGRHVAAGLPDWSANGVRVGAVRVFWLSDDGWTAETMLRHQGTESWNLGCSLALWE
                     NTMVAGARGGLGDAFVYVLNEDGWVQRDRLSAPEGDTNTGFGHAVAMWEEWIAVSAIW
                     GVNDGNPSGAVHLYRQVGEEWIHVERLSSPELDKAGAFGDQLAMAGGRLLISDPAADA
                     PEQDSGVAWLYDLNAKNAATAWSRYP"
BASE COUNT          597 a          961 c         1041 g          532 t
ORIGIN      
        1 caggtgacat ttctattgtg tccagggggt gacattatca ttgtggcccg acagcaggcg
       61 gggggagttg gggcggttgt ggttgtggga gaatgctgtg ggattgggat cgggttcctg
      121 caatcgacgg atttcgagag tcgagcacga gggacgagag ccgggagccg aattctgggg
      181 tgggcctttg ggggcgatca cgaggacgag gacgaggatg agagagacga cagacgaatt
      241 cttgagtgcg gtggggttca gggggcgagg gatggggaga tgggtggggc ggcggtcatt
      301 cccatcgccg ccggcaagcg gtcgccggcc atatcgagca gccagtcgtg atcagcgcgg
      361 ggaagctcaa tcagctcgat ccagtcgtgt tcgcgggcca tttgccgact catctcgacg
      421 ggcacaaggg gatcggcatc gccatggagg atcgtgacgg cggggcgttc gggccgctcg
      481 gcgagggccg ccagcgccgc gcggttgtcg aagcgatggc ggagcagaca ggcgagcggc
      541 cagccgacgg tgcggcgcgc catcgcgagc atgtcggaaa agggcgcgag caggacgatg
      601 cggcgggcgc cggtgtggcg ggcgagctgg agcgcggccg ccgcgcccag ggaataaccc
      661 atcaggtgca gccggtcgtt cagcgcggcg cggtcgaggt tgaggtcgcg ggcgagcgcg
      721 tcgagcgccc cctggctggc ttccaggatc gtgccggggg tgggcttgcc cgcgctcgct
      781 ccgtagccgg gaaagtcgac gaggaggtag gccgccccgg cgtcgggcag cggctcgatc
      841 gtatcgagcc agtccagcgc ccgcgccgcg ttgccgtgga acaggaccca gagccggacc
      901 ggcggcgccg cgtgggcggc aggagcgcgc ggcggcacgt aatacgcctg ctggctgccc
      961 tggctggtct cgaagggaag gaatgtgatt gccccacgca tgcggtcaag ccaggcggag
     1021 tcataccggc tcgggtaata gatcattctg ttctgcagca gaaagatgca gactgagaag
     1081 acgagggcga atccgatgag aatggcgagc ggcatccaga gcatcctgcg cggaatcccg
     1141 gggattcgag gtcgacgcga catggtattc tttctaacct tcgggagggg tggaggcaag
     1201 cgagttcgcc ggggtgcgca tcgagatggc cggcggaagg atcgtgaggg acctgccgat
     1261 gggcccgcgc agtggggacg cacaagaaac cgcccatctg aatcagtctg tggccccgaa
     1321 ggggcgaccg atgaatagcc gtagatgtca acctacggat tccggatcca acattcatcc
     1381 ggtccccgca agggggccta ctcctttcgg catagggaaa cgagaccaac agattcccca
     1441 cgtcaatagt gggtcggtgg cccgtccgaa acgccaagtt ccgtagggac ggctgagaat
     1501 cgattacgag gacgaggacg agggacgagg gacgaatttt ccaggtaggg cgagggacgg
     1561 ggtggtgatt gaagcggaca acgtgtcgaa atcctacgca accgcggatg gcgcggccgg
     1621 tcagatcgtc ctgcgcgacg tttcgttgcg cgtggccggc ggggagttcc tggcgctgat
     1681 ggggtcatcg ggcagcggca agacgacgct gctcaacctg atcggcggac tcgacacgcc
     1741 cgactccggc cgcattcacg tgggcagcca ggatctgcag cgcatgagcg acaatgcgcg
     1801 cagtgcgttc cgcctgcgtc acatcgggtt cgtcttccag ttcttcaacc tgctgccgta
     1861 cctgagcgtg cgcgaaaaca tcgcgctgcc gctcctcttt ctgcgccggc ccgttccgga
     1921 ggcggaggat cggtcctccg cgctggccgc cgaggtggga ctggcggaca aactggatcg
     1981 ccagagcaac caactctcgg gcggcgagat gcagcgtgta gcgctggcgc gggcgctcgt
     2041 tcacgaatgg taatatccgc tgccggctgc atcccatcgg cgcggaagat cacgcgaagg
     2101 gcgaaccgga gtcaataccg gcgccactgc acgaacctgt atccccttct ccccttccgc
     2161 cgccggctcc ccgcgccatc cgacacaatg atgcccgcgc cgcctatggt tggtgtcgcc
     2221 gtgtgaatca accccgtatg acccagccag ccctcgccca tcgcccgctc cccgccgatc
     2281 ggtccttcgt ctcgctcttt gctccctagc ggatccgggc caaaatgcag gccacggtgg
     2341 tcaagtcctt tcaaatagtt ctcgggaaaa cagtctttcc ccccatatgg atcagctagg
     2401 gatagcggga ccaggcagtc gccgcatttt tcgcgttcag gtcgtaaagc catgccacgc
     2461 cggagtcttg ttccggcgcg tcggctgccg ggtcacttat cagcaaccgc cctcccgcca
     2521 tggcgagttg gtcaccgaaa gctccagcct tatccaactc gggtgaagac aaccgctcca
     2581 cgtgaatcca ttcctctccg acctgacggt agaggtgaac ggccccggag gggttgccat
     2641 cattcacccc ccagatcgcg gagacggcta tccattcctc ccacatcgcc accgcatgac
     2701 cgaagcctgt attcgtgtcg ccttcgggcg cagacagccg gtcccgctga acccagccat
     2761 cttcattcag cacatacaca aaggcatcac caagtccgcc gcgggctccc gcgaccatcg
     2821 tattttccca tagagccaga gaacagccaa ggttccagct ctcagtgccc tggtgacgta
     2881 gcattgtctc ggcggtccaa ccgtcatccg acagccagaa gactcgcact gccccgactc
     2941 ttacaccgtt tgcagaccaa tcgggcagac ctgcggccac atgtcggccc gacacagcaa
     3001 ccgaataacc cagaaactca ttctgctgcg cttcaagacc gctgttcaac tctgcttcaa
     3061 gcacccacga atcatccagg tggcgataaa cgaatgctga gcccgaatcg gcgggcacga
     3121 tccactcccc a
//
LOCUS       DAOPCO010000390         3073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_25228_length_3073_cov_5.004307, whole genome shotgun sequence.
ACCESSION   DAOPCO010000390 DAOPCO010000000
VERSION     DAOPCO010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25228_length_3073_cov_5.004307"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..812)
                     /gene="rpe"
                     /locus_tag="PLS90_16770"
     CDS             complement(66..812)
                     /gene="rpe"
                     /locus_tag="PLS90_16770"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017814699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPK04100.1"
                     /translation="MSPATRSKKQPAPIFVPYDPNTAGVPVVIAPSLLSSDFAWAARE
                     LARCRRAGARWLHVDIMDGHFVPNITFGPPVLKKWVAAEPDLFYDTHLMIEHPMQYAE
                     RFVEAGSGLITIHVETVGRPRRELRALRRLGVKAGVVIKPRTPVREIQSLLDEVDLVL
                     VMTVEPGFGGQALIPSTLNKVRELDLIRRRESLPFRLQVDGGINAETAEMAVAAGADV
                     LVAGNAVFANGDIAGNLRALRQALARIKPT"
     gene            1353..2738
                     /locus_tag="PLS90_16775"
     CDS             1353..2738
                     /locus_tag="PLS90_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04101.1"
                     /translation="MLSLLLTSGGPTPAAQTLGFGSPGPLNSNAASDSGSDLVPVIAS
                     GGSGIWIAVWQSDDSLGGTLGADSDILYARSTDNGVSWTAPAALNSNAASDSGSDEDC
                     AIAGDGNGNLIVVWTSLDSLGSTIGTDEDILFSRSSDNGANWTAPAAIASYAPSDSDF
                     DNKPAIATDGAGRWVVLWQSSNSLGGAIGVDLDILYVRSTDNGANWSAPAAFNSNAAS
                     DSGQDYYVTLTADGQGLWIGAWTSTDSLGNTTSFDPDLLFTRSIDDGANWSAPQALNS
                     NAGSDSGDDYRAALAADGAGNWLVAWVSTDSLGGTLGTDQDILFSRSSNDGTSWTSPA
                     PVHDNADSDSTALDERPSLAVDADGNWLVCWQSTANLGGALGNDFDLLVSQSTDLGAS
                     WTAPAALADNADTDIGADEAPTILAHPDGTWLVVWNSADYLGSTIGLDNDILVSSSMS
                     VPIRFRRLTVD"
     gene            complement(2808..>3073)
                     /locus_tag="PLS90_16780"
     CDS             complement(2808..>3073)
                     /locus_tag="PLS90_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPK04102.1"
                     /translation="VQSGEPAEAGFGGAAEENRLGLPLLVLLSVTGVVTIGTLVGSML
                     PFFFKRIGLDPAVCSGPFIATFVDVSGIVLFFTLFRWIFGGYL"
BASE COUNT          531 a         1088 c          896 g          558 t
ORIGIN      
        1 gtcaatccat cgaacgtgcc cggatacagc gcacggtgtt ccttgtcggc cgacatggga
       61 ttgcctcagg tgggtttgat ccgcgccagc gcctggcgca gggcgcgcag attgccggcg
      121 atgtcgccgt tggcgaatac ggcgttgccc gccaccagca cgtcggcgcc cgccgccacc
      181 gccatctccg ccgtctcggc attgatcccc ccatccacct gcaggcggaa cggcaggctt
      241 tcgcggcgcc ggatcaggtc cagctcgcgc actttgttga gcgtcgacgg gatcaacgcc
      301 tggccgccga atcccggctc gaccgtcatg accagcacca gatccacttc gtcgagcagc
      361 gactggattt cgcgcacagg cgtccgtggc ttgatcacga cgccggcttt cactcccagc
      421 cggcgcagcg cgcgcagttc ccgccgggga cgcccgacgg tctccacgtg gattgtgatc
      481 aggcccgagc ccgcctcgac gaaccgttcg gcatactgca tcgggtgttc gatcatcaga
      541 tgcgtgtcgt agaacaggtc gggctccgcg gcgacccact tcttcaagac cggcggacca
      601 aatgtaatgt tgggaacgaa gtggccatcc atgatgtcga cgtgcaacca tcgggcccct
      661 gcgcgccggc aacgggcgag ttcgcgcgcc gcccaggcga aatcgctgct cagcagcgat
      721 ggcgcgatga cgaccggcac gccggccgtg ttcggatcgt agggcacgaa gatcggtgcg
      781 ggttgcttct tgcttcgagt tgcgggtgac atggcggatg ggccggatgg aaatctcgcc
      841 ggaccgcgcg cgagcggccg cggcgtgtcc atcctccgca tccgctccaa aatagcaagt
      901 ccggcgacaa cgcccgatga tagagtgccc ttcgtggcgc ccccggttcc tgggcaactc
      961 atcctcgtgc ttcggtcgtt cttccgttcc ttgtgtcttt atttcctttc tgtcctttct
     1021 gcccccttac tgtccccaaa ggctgaatga ttgttgacag atcatgacct cttggtctat
     1081 aaaacgctcg ttcggaagga acgaatgcac tcgtccttca aattccggct ctccaaacgc
     1141 cgccaccaat tcctcctgcc cttccccggt tctcgttccc tcgaacggga actttttact
     1201 atccggcgat ggtgcatacc gttccagggc aggattccgg cgccgtgtgt catggaaatc
     1261 tgatgccctg ttatcatctt tttgcccaac gcatcaggca cgccgcaaag cggtttgctt
     1321 cctcccggcg cactgccgcc ttcgtggcgc tgatgctctc cctcctgctc acgagcggcg
     1381 gtcccacgcc cgctgcgcag acgctgggct tcggttcccc cggccccctc aacagcaatg
     1441 cggcgagcga ttcgggaagc gacctggtcc cggtcatcgc ctcgggcgga agcggcatct
     1501 ggattgccgt ctggcaatcg gacgattcgc tgggcggcac gctcggcgcc gattcggata
     1561 tcctctatgc ccgctcgacc gacaatggcg taagctggac cgctcccgcg gccctgaaca
     1621 gcaatgccgc ctccgattcg ggcagcgacg aggactgcgc catcgccggc gacggcaacg
     1681 gcaacctgat cgtcgtctgg acctcgctcg attcgctcgg cagcacgatc ggcaccgatg
     1741 aggatattct cttttcccgc tcgagcgaca acggagcgaa ttggaccgcc ccggcggcca
     1801 tcgccagcta cgccccgtcc gattcggatt ttgacaacaa gcccgccatc gccaccgacg
     1861 gcgccggccg ctgggttgtc ctctggcaat cctccaactc gctgggcggc gccatcggtg
     1921 tcgacctgga catcctctat gtccgctcga cagacaacgg cgccaattgg tcggcgcccg
     1981 ccgccttcaa ttccaatgcc gcctccgatt ccggtcagga ttactatgtc acgctgacgg
     2041 ccgacggcca gggcctctgg atcggcgcct ggacctccac cgattccctg ggcaacacga
     2101 ccagcttcga ccccgatctg ctcttcaccc gctcgatcga cgacggcgca aactggtccg
     2161 cgccgcaggc cctcaacagc aacgcgggct ccgattcggg cgatgactat cgcgccgccc
     2221 tcgccgccga cggcgcgggc aactggctcg tggcctgggt ctcgaccgac agcctcggcg
     2281 gcacgctcgg cacggaccag gatattcttt tcagccgctc aagcaatgac ggcacgagct
     2341 ggacctcccc cgcgccggtc cacgacaacg ccgactccga ttcgaccgcg ctggatgaac
     2401 gtcccagcct cgccgtcgat gccgacggca actggctggt ctgctggcag tcgaccgcca
     2461 acctgggcgg cgcgctcggc aatgacttcg atctgctggt gagccagtcc accgacctgg
     2521 gcgcgagctg gactgcgccg gccgcgctgg ccgacaatgc cgacaccgat atcggcgcgg
     2581 acgaagcccc gacgatcctg gcccatcccg acgggacatg gcttgtcgtg tggaactcag
     2641 ccgactatct gggttcgacg atcggcctcg acaacgacat cctggtcagc tccagcatgt
     2701 cggttccgat ccgcttccgc cgcctcacgg tggattgaca agagtcggtt gagacctgcg
     2761 acacgaacgg gacgtatgcc cattcgcctc tttcgtgacc caaaaagcta cagataaccg
     2821 ccgaaaatcc agcggaacag cgtgaagaac agcacgatgc ccgacacgtc gacaaatgtc
     2881 gcgatgaacg gcccggagca gaccgccgga tccagcccga tccgcttgaa gaaaaacggc
     2941 agcatcgaac cgaccagcgt cccgatcgtc accacgccgg tcacgctcag cagcaccagc
     3001 agcggcagac cgagccggtt ctcctcggcc gcgccgccga accccgcttc ggccggttct
     3061 cccgactgaa cgg
//
LOCUS       DAOPCO010000391         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_25596_length_3039_cov_3.998995, whole genome shotgun sequence.
ACCESSION   DAOPCO010000391 DAOPCO010000000
VERSION     DAOPCO010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25596_length_3039_cov_3.998995"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PLS90_16785"
     CDS             <1..1091
                     /locus_tag="PLS90_16785"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HPK04103.1"
                     /translation="VNMGPQHPSTHGVLRLVVRTDGEIVREAWPQIGFLHRCFEKVAE
                     SVTYAQVTPYCDRMDYLASMNNGLAWCLAVERLGGIEVPERAAYLRVMMAELNRIASH
                     LLAFGTYALDLGAFTPFLYGFRERELILSIFEKISGGRLLYHYPRLGGVARDLTPELA
                     GEIRAFIAQMRRAWPEYNELLSENQIFIKRTANVGVIPAAMAVAYGLTGPCLRGSGVK
                     FDLRRAKPYSLYERFDFDIPVGEGLKGEVGDCWDRYWVRMREIIESLRIIEQALDGLP
                     EGEIQTKVSRTLKLPAGEVYVPCENPRGELGYYLISDGKTSPLRVKARGPSFNNLSIT
                     HALVRDVLIADVVAIVGSIDIVLGEVDR"
     gene            1160..2335
                     /locus_tag="PLS90_16790"
     CDS             1160..2335
                     /locus_tag="PLS90_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPK04104.1"
                     /translation="MPAWLGYSVLLLLGAALIIGWMSLLALAWTYGERRIAGLIQVRL
                     GPNRVGPQGILQPLADGIKLLVKEDVVPAVAYRFIYTLAPLIVFVGALLPFIVLPFSE
                     RLVISNIELGLYFVLAFEAIEVIGVLMAGWAPGSKWSLYGGMRLAAQMLSYEIPMGLC
                     ALVIIILTRSVNLGEIVAWQSRDAWFMEHPWIFGWAIFRSPAALLAFVMFYVGGLAAT
                     KRAPFDLPEAESELVAGYHTEYSGMRFAFFFMAEYASMYVICALATILFLGGWHAPIP
                     RPELAADASLMEIWSRLAAAHGAAGTGVAGKFGAFGGSLIALLTDPGALKILLHQTFG
                     VVNLLLKTFLLYFVMIWVRWTLPRLRIDQVMYLCLKVFLPFSVACVVWALIQVVILG"
     gene            2481..>3039
                     /locus_tag="PLS90_16795"
     CDS             2481..>3039
                     /locus_tag="PLS90_16795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HPK04105.1"
                     /translation="MKDYFRNLWAGIVTTWVGMRLTLRYLIKPSVTLRYPEERPVIPA
                     GYRGIHGYGEEKCSVCKSCAAACPVDCITIEQLGRGKDAMVMRFEVDYSRCLFCDLCT
                     IPCPTQCIWLSERYDLAGYTREECRIDFARPKSPEEIAAHEKLLRKKEEEKKAKAAAA
                     AAAKAKKETETKAAAEASAPQAGDAP"
BASE COUNT          513 a          849 c         1083 g          594 t
ORIGIN      
        1 tggtcaacat gggcccgcag catccgtcga cgcacggcgt gctgcggctg gtcgtgcgga
       61 ccgacggcga gatcgtgcgc gaggcgtggc cgcagatcgg cttcctgcac cgctgcttcg
      121 agaaggtcgc cgagtcggtg acctacgcgc aggtgacgcc ctactgcgac cggatggatt
      181 acctggcgtc gatgaacaac gggttggcgt ggtgcctggc ggtggagcgg ctcggcggga
      241 tcgaggtgcc ggagcgggcg gcgtatctgc gcgtgatgat ggccgagctg aaccggatcg
      301 cgtcgcacct gctggcgttc ggcacctatg cgctcgatct gggcgcgttc acgccgtttc
      361 tgtacgggtt ccgcgagcgc gagctgatcc tgtcgatctt cgagaaaatc agcggcgggc
      421 ggctgctcta ccattatccg cggctggggg gtgtggcgcg cgacctgacg ccggaattgg
      481 caggggaaat tcgcgcattc attgcgcaga tgcgccgggc gtggcccgaa tacaacgagc
      541 tgctgagcga gaatcagatt ttcatcaagc ggacggcgaa cgtcggcgtc attccggcgg
      601 caatggcggt ggcctacggg ctgaccggcc cgtgcctgcg cggcagcggc gtgaaattcg
      661 acctgcgccg ggcaaaaccc tattcgcttt acgaacgatt cgacttcgac atcccggtgg
      721 gcgagggcct caagggcgag gtcggcgact gctgggatcg ctactgggtg cggatgcggg
      781 aaatcatcga aagcctgcgg atcattgagc aggcgctcga cggcctgccc gagggggaga
      841 tccagaccaa ggtgagccgg acactgaagc tgccggcggg cgaagtgtac gtgccctgcg
      901 aaaatccgcg tggcgagttg ggctactacc tgatttcgga cggaaagacc tcgccgctgc
      961 gcgtgaaggc gcgcgggccg tcgttcaata atctgagcat cacgcacgcg ctcgtgcgcg
     1021 acgtgctgat cgccgatgtc gtcgcgatcg ttggctcgat cgacatcgtg ctgggcgagg
     1081 tggaccgctg atggaagcga cggccgcgga catttttctg cagcgggtgc tgggggcgat
     1141 ctggcccggc gcgcaaacga tgccggcctg gctcggctac agcgtgctgt tgctgctcgg
     1201 cgcggcgctg atcatcggct ggatgagtct gctggcgctg gcctggacgt atggcgagcg
     1261 gcggatcgcg gggttgatcc aggtgcggct cggacccaac cgggtagggc cgcagggcat
     1321 cttgcaaccg ctggccgacg ggatcaagct gctcgtcaag gaagacgtcg tccccgcggt
     1381 ggcgtatcgc ttcatctata cgctggcgcc gctgatcgtg tttgtcggcg cgttgctgcc
     1441 gttcatcgta ctgccgttca gcgagcggct cgtgatcagc aatattgaac tggggcttta
     1501 ttttgtattg gcttttgagg cgattgaagt gatcggcgtg ctgatggcgg gctgggcgcc
     1561 gggcagcaaa tggtcgcttt acggcgggat gcggctggcg gcgcagatgc tctcctacga
     1621 aattccaatg ggtctgtgcg cgctggtgat cattattctg acgaggagcg tgaacctggg
     1681 tgaaatcgtc gcgtggcaat cgcgcgacgc gtggttcatg gagcacccgt ggatcttcgg
     1741 ctgggcgatt ttccgttcgc cggcggcatt gctggcgttc gtcatgttct acgtcggcgg
     1801 gctggccgcc accaaacggg cgccgttcga cctgcccgaa gccgaaagcg aactggtcgc
     1861 cggctaccac acggaatact cgggaatgcg tttcgccttt ttcttcatgg cggaatacgc
     1921 ctcgatgtat gtgatctgcg cgctggcgac gatcctgttc ctgggcggct ggcatgcgcc
     1981 gatcccgcgg ccggagctgg cggccgatgc atcgctgatg gagatctggt cgcggctggc
     2041 ggcggcgcac ggggcggccg gcacgggggt ggcgggcaaa ttcggcgcct tcggcggcag
     2101 cctgatcgcg ctgctgaccg accccggcgc gctcaagatc ctgctgcatc agactttcgg
     2161 cgtcgtcaat ctgctgctca agacgttcct cctctacttc gtgatgatct gggtccgttg
     2221 gacgctgccg cgactgcgga tcgaccaggt gatgtatctc tgtctgaagg tttttctgcc
     2281 gttttcggtg gcctgcgtgg tgtgggcgct gattcaggtg gtaatactgg ggtgatgggg
     2341 gagctcgtgg tgaatatgag agagaaggac gatggttgtt cgagagccga aaacgagaga
     2401 cgagagacga gaggcgaatt tagggcgagg gtcgatcgag gattgattga cttatgttga
     2461 tgtcttcaac ggcacatccc atgaaggact acttccgcaa cctgtgggcg gggatcgtca
     2521 ccacgtgggt cgggatgcgc ctgacgctgc gctacctgat caagccatcc gtcacgctcc
     2581 gctatccgga agaacggccg gtgattccgg cgggctatcg cggcatccat ggctatggtg
     2641 aggagaaatg ctccgtttgc aagtcgtgcg cggcggcctg cccggtcgat tgcattacaa
     2701 ttgaacagct tggccgcggc aaggacgcga tggtgatgcg gttcgaggtg gattacagcc
     2761 gctgcctgtt ctgcgatctg tgcacgatcc cgtgcccgac gcagtgcatc tggctgtcgg
     2821 agcgctacga cctggcgggc tacacgcgcg aggaatgccg gattgatttc gcgcggccga
     2881 aatcgcctga ggaaatcgcg gcacacgaga aattgctacg gaagaaggaa gaggaaaaga
     2941 aggccaaggc cgcggcggcg gcggccgcca aggcgaaaaa ggaaaccgag accaaggccg
     3001 cggccgaggc ctccgctccc caggccggcg acgccccga
//
LOCUS       DAOPCO010000392         3034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_25645_length_3034_cov_4.732125, whole genome shotgun sequence.
ACCESSION   DAOPCO010000392 DAOPCO010000000
VERSION     DAOPCO010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25645_length_3034_cov_4.732125"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLS90_16800"
     CDS             complement(<1..461)
                     /locus_tag="PLS90_16800"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPK04106.1"
                     /translation="MNRKIRIATSWLDGCSGCHMSFLDIDERLLTVLDKFEIVYGPLV
                     DAKQVPEGIDVALVEGAVSNQDDLELIQQLRARSSLLISLGDCAVTSNVPAMRNPHPL
                     GKILERVYIENAQADQQIPANGVPPLLRHARPLHEVVKVDLHIPGCPPEPDL"
     gene            complement(458..1165)
                     /gene="hoxU"
                     /locus_tag="PLS90_16805"
     CDS             complement(458..1165)
                     /gene="hoxU"
                     /locus_tag="PLS90_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HPK04107.1"
                     /translation="MDRVNLKINGQPVQARAGQSLLAVARDNGIDIPTLCHLDGLSGV
                     GACRLCLVEVAGVGRLLPACTTPVQHDMDVRTDSPRLARYRRMILELLFSERNHVCAV
                     CVSNGHCDLQAMAQSQGMTHVRFPYLYPPLKVDASHRRYVLDHNRCILCTRCVRVCAE
                     VEGAHMWDVMSRGVHSRLVAELNQPWGDSQVCTSCGKCVAVCPTGALAEKGRAVGEMQ
                     KPPLHLTDLALLRGGLS"
     gene            complement(1216..>3034)
                     /locus_tag="PLS90_16810"
     CDS             complement(1216..>3034)
                     /locus_tag="PLS90_16810"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPK04108.1"
                     /translation="DYESLAQIGSIMGSGGMVVMDEDNCMVDVARYFIEFTHSESCGK
                     CVPCRVGLEHALRILNNVSQGAAATTDLEMLDELGRMIRDTSLCGLGQTAPNPVLTTL
                     RHFMEEYTDHIRGHRCPAGVCEHLALSPCENSCPLHMNIPRFLQLLKEERLEDAFESV
                     ILDNPLPASTGRVCQHPCDNRCRRVSLDQAVNMRDVHRHIADSVLFSDSFDAMAERLV
                     ARRLEPTGRRAAVVGAGPTGLTCAFYLALLGHDVTVYDSNPLPGGMLRYALPEYRLPK
                     QVLDREVELIRRLGVNFVGNVRVGDDLSLNQLDDQFDSVFISIGTWKEAWVYLAGTEL
                     KGVIPALPFLEAVARDSHVALGRRVIVIGGGNAAIDSARTALRMGSEVTVVYRRERRD
                     MPAIPEEIAAAEAEGVRFVFLAAPHRILGDPQGNVKAIEVVKTELGEFDRSGRRTPIL
                     TDEIYRFECDSVVLAVGERVDLDFAKASGLKIKESGTIEIDRYTMETSRRKFYAGGDV
                     VTGASNVSNAMAYGKQAAAVIDQFLTGERRFDRIFRAFNYSKLPPEPVDTPRHRLAEL
                     PPQARVRGFEEVMLGLSRADAVAETHRCLRCDIKEVSPV"
BASE COUNT          495 a          851 c         1197 g          491 t
ORIGIN      
        1 aggtcgggct cgggaggaca gccggggata tggagatcga ccttgacgac ttcgtgcagc
       61 gggcgggcgt ggcgcaagag cggcgggacg ccgttggccg ggatctgctg gtcggcctgg
      121 gcgttctcga tatagacgcg ctcgaggatc ttgccgagcg gatgcgggtt gcgcatggcg
      181 gggacgttgc tggtgacggc gcagtcgccg agcgagatga gcaggctgga ccgggcgcgc
      241 agttgctgga tcagctccag atcgtcctgg ttgctgacgg cgccctcgac cagtgcgacg
      301 tcgatgccct cggggacctg cttggcgtcg accagcgggc cgtagacgat ctcgaacttg
      361 tcgaggacgg tcaggagccg ctcgtcgata tcgaggaacg acatgtggca acccgagcag
      421 ccatcgagcc aactggtggc gatgcggatc ttccggttca tgagaggcct ccgcgcagca
      481 gggcaagatc ggtgagatgg aggggcggtt tttgcatttc gccgacggcg cggccctttt
      541 cggcgagggc gccggtgggg cagaccgcga cgcatttgcc gcaggacgtg cagacttgag
      601 agtcgcccca gggctggttc agttcggcga cgaggcgcga gtggacgccg cggctcatca
      661 cgtcccacat gtgggcgccc tccacctcgg cgcagacgcg cacgcagcgc gtgcagagga
      721 tgcagcggtt gtgatccagc acgtagcgcc ggtgcgaggc gtcgaccttg agcggggggt
      781 agagataggg gaagcggacg tgggtcatgc cctgggactg ggccatggcc tgcaggtcgc
      841 aatggccgtt ggagacgcag acggcgcaga cgtgattgcg ttcggagaag aggagctcca
      901 ggatcatgcg ccggtagcgg gcgaggcgcg gcgaatcggt gcggacatcc atgtcgtgct
      961 ggaccggcgt ggtgcaggcg ggcagcaggc ggccgacacc ggcgacctcg accaggcaga
     1021 ggcggcaggc gccgacgccg gaaagaccgt cgaggtggca gagggtcggg atgtcgatgc
     1081 cgttgtcgcg cgcgacggcg agcagggact ggccggcgcg ggcctgaacc ggttgaccat
     1141 tgatcttcag attgactcga tccaccttga gcgactcctc gaagcaaagg gttgcgcgcg
     1201 ccggttggag cggcgtcaga cggggctgac ttccttgata tcgcagcgca ggcagcgatg
     1261 ggtttcggcg acggcgtcgg cgcgcgagag gccgagcatc acctcctcga agccgcggac
     1321 gcgcgcctgg gggggcagtt cggccaggcg gtggcgcggg gtgtcgaccg gctcgggcgg
     1381 aagcttgctg tagttgaacg cgcggaagat gcggtcgaag cggcgctcgc cggtgaggaa
     1441 ctggtcgatg acggcggcgg cctgtttgcc gtaggccatg gcgttggaga cattggaggc
     1501 gccggtgacg acgtcgccgc cggcgtagaa tttgcgccgg ctggtctcca tcgtgtagcg
     1561 gtcgatctcg atcgtgcccg attccttgat cttgaggccg gaggccttgg cgaaatcaag
     1621 atcgacacgc tcgccgacgg ccagcacgac gctgtcgcac tcgaagcggt agatctcgtc
     1681 ggtcaggatg ggggtgcgcc gcccggagcg gtcgaactcg cccagttcgg tcttgacgac
     1741 ttcgatggct ttgacgttgc cctgcgggtc gccgaggatg cggtgggggg cggcgaggaa
     1801 gacgaaacgg acgccttcgg cctcggcggc ggcgatttcc tcggggatcg cgggcatgtc
     1861 gcgccgttcg cggcggtaga cgacggtgac ctcggagccc atgcgcaggg cggtgcgggc
     1921 cgagtcgatc gcggcattgc cgccgccgat cacgatgacg cggcggccga gcgcgacatg
     1981 gctgtcgcgg gcgaccgctt cgaggaaggg aagagctggg atcacgccct tgagctccgt
     2041 gcccgccagg tagacccagg cttccttcca ggtgccgatg gagatgaaga cggaatcaaa
     2101 ctggtcgtcg agttggttga gcgagaggtc gtcgccgacg cggacgttgc cgacgaagtt
     2161 gacgccgagg cggcggatca gttccacctc gcggtcgagg acctgtttcg gcaggcgata
     2221 ctccggcagg gcgtagcgca gcatgccgcc gggcaggggg ttgctgtcgt agacggtgac
     2281 gtcgtggccg aggagcgcga ggtagaacgc gcaggtcagg ccggtgggcc cggcgccgac
     2341 gacggcggcg cgcctgccgg tcggctcgag gcggcgcgcg acgagacgct cggccatcgc
     2401 atcaaagctg tcggagaaga gaaccgaatc ggcgatatgg cgatggacgt cgcgcatgtt
     2461 gacggcctga tcgaggctga cgcggcggca gcggttgtcg caggggtgct ggcagacgcg
     2521 gccggtggag gccggcaggg ggttgtcgag gatgaccgac tcgaacgcat cctcgaggcg
     2581 ttcctccttg agcagttgga ggaagcgggg gatgttcatg tggagcgggc agctgttctc
     2641 gcagggcgaa agggcgaggt gttcgcagac gccggcgggg cagcgatgcc cgcggatgtg
     2701 gtcggtgtat tcctccatga agtggcgcag ggtggtcagc acggggttgg gagccgtctg
     2761 gcccaagccg cagagcgagg tatcgcggat catgcggccc agctcgtcga gcatctcgag
     2821 gtcggtggtg gccgcggcgc cctgggagac gttgttcagg atgcgcaggg cgtgctcgag
     2881 gccgacgcgg caggggacgc acttgccgca cgattcggaa tgggtgaatt cgatgaagta
     2941 gcgggcgacg tcgaccatgc agttgtcttc gtccatgacg accatgccgc ccgatcccat
     3001 gatcgagccg atctgggcga gcgattcata atcg
//
LOCUS       DAOPCO010000393         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_25992_length_3001_cov_2.644263, whole genome shotgun sequence.
ACCESSION   DAOPCO010000393 DAOPCO010000000
VERSION     DAOPCO010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25992_length_3001_cov_2.644263"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PLS90_16815"
     CDS             complement(<1..1471)
                     /locus_tag="PLS90_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04109.1"
                     /translation="MDMNSSIFSSENWKHFLRQSRSRHQYHLVVVVCLVGMVLVSRGI
                     LAGLEPPDRRRPRFAFDDLPTAEVLVLGNSQLQRILPDELSLKTINSAEGAADYSVQD
                     AVLRSLAPRMPRLRHVIVGLDNIPLHLAAIEERHGDYSDIMSLGVPWWRIPDVGPVDR
                     LTYSIATSRFLKPFFRGPKLDYPGLESLIHSMLEPRSDSNESALSPGPAPDSPSAQPR
                     VLRAGLLSFDLAYPRLTFPIGLLPVTSAVADASTTPAQVDSTTPIPETPASAPRPPQY
                     PIRPDEGHLKIRGYIRKFESKDHFEANFRAFTRIVSYCDARNLNLIFVRVPTSRSFWS
                     NRPAQWDRELEMLKAAAASILHPKPLIVLDEESRYNYPDSYFNDPNHMTQAAAMEYTR
                     RLNERLLAIDERLDATGYQTASTQADAPEQTDVTTHIERNGQELLLNPEFAFWRGGEP
                     VSWHSSPRKDLVNRVDKLNGEHCVELAPGPQGSRSSLYQL"
     gene            complement(1440..2849)
                     /locus_tag="PLS90_16820"
     CDS             complement(1440..2849)
                     /locus_tag="PLS90_16820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPK04110.1"
                     /translation="MLFNSILFLLFFCIVYLLYVSLKHRGQNLLLLVASYLFYGTWDW
                     RFLILIFLTTLVDYTTALKVYHVASPRRRRIYVAISITFNLSILFFFKYFNFFVDSTL
                     SLFHFFGLNPDIVTIKILLPVGISFYTFQSMSYTIDVYRGQLTPTRSLLDYMLFVSFF
                     PQLVAGPIERARELLPQVQAPRCIDHAMVREGLWLILLGYFKKVVIADNMAPIANEVF
                     NNPDAHQGLPVLLGIYAFAFQIYGDFSGYSNIARGVARLMGFHLMVNFATPYFALNPR
                     DFWRRWHISLSTWLRDYLYIPLGGNRKGPVRTYANLSITMLLGGLWHGAAWNFVAWGL
                     YHGLLLILHRLAEHVGFWRSENAALSRPLRLLQIGVMFHLTCLSWVLFRINHLSDFPV
                     LMGNLFTASLDHVSWLWIIILITGPLLIFEIAWRRSDDLLKVKSLPPAARLAIYCLLL
                     GYILLTGKGDGYEFIYFQF"
BASE COUNT          717 a          785 c          937 g          562 t
ORIGIN      
        1 ccagttgata gagactggag cgacttccct ggggacccgg cgccagttcc acgcaatgct
       61 cgccattcag cttgtcaacc cggttgacca gatccttccg gggtgagcta tgccaactga
      121 cgggctcgcc tccgcgccag aacgcgaatt caggattaag cagcaactcc tgaccgttgc
      181 gctcgatatg ggtcgtgacg tcagtttgct ccggagcatc ggcttgcgtt gaggctgtct
      241 ggtaacccgt tgcgtcgaga cgttcgtcga tcgccagaag cctttcgttc agccggcggg
      301 tatattccat cgcagccgcc tgggtcatgt gattcggatc attgaaatag gaatccggat
      361 agttataccg gctctcctca tccaggacaa tcaacggctt gggatgcagg atggatgcgg
      421 ccgcggcctt caacatttcg agctcacgat cccattgagc ggggcggttg ctccagaagg
      481 aacgcgatgt cgggacgcga acgaaaatca gattcagatt tcgtgcgtcg cagtatgaga
      541 caattcgggt aaaggcccgg aagtttgctt cgaaatgatc tttcgactca aactttctga
      601 tataaccacg gatcttcaag tgcccttcat ccggtcgaat agggtattgc gggggacgcg
      661 gcgcgctggc cggcgtctcc gggatcggcg tcgttgaatc cacctgggcg ggcgtcgttg
      721 aggcgtcggc gacggccgat gtgaccggca atagaccgat ggggaaggtg agccgcggat
      781 aggcgagatc aaagctcaag agtcccgccc gcagaacgcg gggctgcgcc gatggcgaat
      841 cgggcgctgg gccgggggac aacgccgatt cgttcgagtc ggagcgaggc tccagcatgg
      901 aatggatgag cgattccagg ccggggtagt ccagcttcgg tccccggaag aagggcttga
      961 ggaacctgga cgtggcaatc gaatacgtta ggcgatcaac gggaccgaca tccggaatcc
     1021 gccaccaggg cactcccagg ctcatgatgt cgctgtagtc gccatggcgc tcctcgatcg
     1081 ccgccaggtg cagcgggata ttgtcgaggc cgacgatcac atggcgcaga cggggcattc
     1141 gcggcgcgag cgaacggagc acggcatctt gaaccgaata atccgccgcg ccttcggcgg
     1201 aattaatggt cttgagagac aactcgtccg gtaatatccg ctgcaattgc gagtttccga
     1261 gaaccaggac ctcggccgtg ggcagatcat caaaggcaaa acggggccgg cgccggtcgg
     1321 ggggctccaa ccccgccaga atgccgcgac tgaccaacac catgccaacc aggcaaacca
     1381 cgacaaccag gtgatactga tgccgcgacc ggctttgccg gaggaaatgt ttccagtttt
     1441 cagaactgaa aatagatgaa ttcatatcca tcgcctttgc cggtcaacag gatatatccg
     1501 agtaagaggc aataaatcgc gaggcgcgcc gcgggcggaa gactcttgac cttgagcaag
     1561 tcgtccgagc ggcgccacgc gatttcgaag atcagcaatg ggcccgtgat gaggatgatg
     1621 atccatagcc aggaaacatg atccagactc gcggtgaaga gatttcccat cagcacgggg
     1681 aaatcgctca gatggttgat gcggaataag acccagctca ggcaggtcag atgaaacatg
     1741 acgccgattt gcagaaggcg aaggggccgc gacaatgcgg cgttttccga ccgccagaag
     1801 cccacgtgct cggcgaggcg atgaagaatg agcaggagcc cgtggtagag gccccatgcg
     1861 acgaagttcc atgcggcgcc atgccagagt ccgccgagca gcatggtgat cgagagattg
     1921 gcgtaggttc tgaccggccc cttgcgattg ccgccaagcg ggatataaag atagtcccgc
     1981 agccaggttg acaggctgat gtgccacctt ctccagaagt ccctgggatt gagcgcaaag
     2041 tacggcgtgg cgaagttgac catcaggtga aaccccatca gtcgggccac gccgcgggca
     2101 atgttcgaat aacccgagaa atcgccgtaa atctggaagg caaaggcata gatgccgagc
     2161 aggacaggca gtccctggtg cgcgtcggga ttgttgaaaa cctcattggc gatcggcgcc
     2221 atgttgtccg cgatgacgac ttttttgaaa taccccagca ggatcaacca cagcccttcg
     2281 cgcaccatgg catggtcaat gcatcttggc gcctgaacct gggggagaag ctcccgcgcg
     2341 cgctcgatcg ggcccgccac gagctgcggg aagaaggaga caaagagcat gtaatcgagg
     2401 agactgcgcg ttggcgtcag ctggccgcgg tagacatcga tcgtgtagct catcgattgg
     2461 aaggtgtaga acgaaattcc gaccggcagc agaatcttga tcgtcacgat gtcggggttt
     2521 agaccaaaaa agtgaaacag actcagcgtg ctatcgacaa agaaattgaa gtacttgaag
     2581 aagaacagga tggacaggtt gaacgtgatg ctgatcgcca catagattct gcggcggcgc
     2641 ggcgatgcga cgtgatagac cttcagcgcg gtggtgtagt cgacgagggt ggtcaggaag
     2701 atgaggatca gaaatcgcca gtcccaggtt ccatagaaaa gataactggc gacgagcaac
     2761 agcagattct gtccgcgatg tttcagactg acgtagagca ggtagacgat gcaaaagaag
     2821 agcagaaaca gaatcgaatt gaacagcata cgatagactc agaagggaga ctgcgggata
     2881 tcggaagcga cgggtcggcg gttccggtgg gagattgctc ggcccgtcat ttcaggccat
     2941 tcctcacgcg atgtcgatga tcaagatgtt ctattttgaa gtgtgatggt attcacctga
     3001 c
//
LOCUS       DAOPCO010000394         2987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26144_length_2987_cov_4.658595, whole genome shotgun sequence.
ACCESSION   DAOPCO010000394 DAOPCO010000000
VERSION     DAOPCO010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26144_length_2987_cov_4.658595"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PLS90_16825"
     CDS             <1..462
                     /locus_tag="PLS90_16825"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPK04111.1"
                     /translation="RALARLGRQAADRPVATAPTAATGNADGIPWIPFSVETLEELVA
                     RNQTVFVDFTADWCVTCQINERAVLETAPVREAIARHGVVPVKADWTLRDEAIGAVLR
                     QLGASGVPVYVIFPAGRAHEPIVLPTVITAGLVIEKLAEATGGLDVAAATH"
     gene            816..1523
                     /locus_tag="PLS90_16830"
     CDS             816..1523
                     /locus_tag="PLS90_16830"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPK04112.1"
                     /translation="MLSVLRSAAIVAALGLGMLATPILADDPSPVPAQPAEEIMEGET
                     SAEVEVSVAAENETVQVGDMVKDFSLTDIDGKTHSLSDYEGKIVVLSWTNPDCPFIVD
                     HNKKRTVDALAEKYADKDVVVLQIDSTHTGTTERLVNMREEYDVKSPILEDYDGSIGR
                     YFGAKTSPHVFVIDTEGKLVYSGAIDNAPMGKLNDGESENVNYAEAVVEDLLAGREVS
                     YSTTKPYGCSVKYADAP"
     gene            complement(1720..2031)
                     /locus_tag="PLS90_16835"
     CDS             complement(1720..2031)
                     /locus_tag="PLS90_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006170959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HPK04113.1"
                     /translation="MGSNPCENQEPTQIILRDQLAIDRTVLAFERTYLAYVRTGLTFF
                     IAGVTFIKLLEGVIVQALGWVFLPIGLLVALLGYRRYRQIKRSLDACGKLTRSTDAGL
                     M"
     gene            complement(2069..2740)
                     /locus_tag="PLS90_16840"
     CDS             complement(2069..2740)
                     /locus_tag="PLS90_16840"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPK04114.1"
                     /translation="MTPPSIPPASSDAPPAEGAQAALSGIILAGGRSSRYGAPKDAAV
                     LAGQTLLEHVARTVGGLCRELIVVTRPGQAVELTRPARRVTDDPDAPEGPLRGIVAGL
                     AAARCELAVVVACDCPLIEPGLVRLLLARAGPELDAVVPIWRDHPQPLHAIYRVAALP
                     ALRALLAAGEASPTRALARLRVDRVAEHDWRGADPDARSFRNINTPADLELVRQAVQQ
                     DRPPA"
BASE COUNT          539 a          967 c         1009 g          472 t
ORIGIN      
        1 cgggcgctgg ctcggctggg tcgccaggcg gcggatcgcc ccgtcgcaac ggcgccgacg
       61 gcggcgacgg gaaacgcgga cggcatcccc tggatcccgt tcagcgtgga gaccttggag
      121 gagctggtcg ctcgcaacca gacggtcttc gttgatttca cggccgattg gtgcgtcacc
      181 tgccagatca acgagcgcgc ggtgctggag acggcgccgg tgcgcgaggc gatcgcgcgc
      241 cacggcgtcg ttccggtcaa ggccgactgg acgctgcgcg acgaggcgat cggcgccgtc
      301 ctgcgccagt tgggggcgtc cggcgtgccg gtctacgtga ttttccccgc cgggcgggct
      361 cacgagccga tcgtgctgcc gacagtgatc acggcggggc tggtgatcga aaaactggct
      421 gaggccacgg gcgggcttga cgtggcggcc gcgacccatt aagctgacgt ggcgaccgcg
      481 caccacgggc ctttgttccc gggaggggac agatcggagg ttcgcccctt cgggggcgat
      541 gatgtctcgc gatgattcac cgcctttggg ggccgatcac gagggacgag gacgcgagac
      601 ggaatttgag agtcaggctg gaccaggggt gcgccagaaa tacacgctgc gtgggcactt
      661 cttgcaaatg accggcggcg cggcgcatcc cagggatcgg tgaatcaggt aaattcgcgg
      721 tttcagcgcc cgtcggcaag atcaacccga ttcggcactg ctcttgcttg atggatcgcg
      781 caacgtcacc ccgatgtgaa cagggaggaa atgtcatgct ctcggtttta cgttcggcgg
      841 ccatcgtagc cgctctcgga ttgggtatgc tggcgacgcc gattctggcg gacgatccga
      901 gccccgttcc ggcccagccg gccgaggaga tcatggaggg cgaaacctcg gccgaggtcg
      961 aggtctccgt cgccgccgaa aatgagaccg tccaggtcgg cgacatggtc aaggatttct
     1021 cgctgaccga catcgacggc aagacccata gcctttcgga ctacgagggc aagatcgtcg
     1081 tcctgagctg gaccaatccg gattgcccgt tcatcgtcga tcacaacaag aagcggacgg
     1141 tcgatgcgct ggccgagaaa tacgccgaca aggatgtggt cgtgctgcag atcgacagca
     1201 cccacaccgg cacgaccgag cggctcgtca acatgcgcga ggagtacgac gtgaaatcgc
     1261 cgatcctgga agactatgac ggctcgatcg gccgctactt tggcgccaag acgagccccc
     1321 atgtcttcgt gatcgacacc gagggcaaac tggtctattc cggcgcgatc gacaatgcgc
     1381 cgatggggaa actgaatgac ggcgaaagcg agaacgtgaa ttatgccgaa gcggtggtcg
     1441 aggatctgct ggccggacgc gaggtcagct actcgacgac caagccgtat gggtgttcag
     1501 tcaaatatgc ggatgcgccg taggtcccgt cagcccccaa gctgaccgtt ccggcatgaa
     1561 cctcccgtat cccccccaac cccttcatgc cgggacaccc caagggcctg caggccgcgg
     1621 tccggtccgg aaacacaggg tcttctccgc cctccttccc cctcccaaca aagccagcgg
     1681 agaggatctt tccggaccgg accgctaagt ttttgcccat cacatcagcc ccgcatccgt
     1741 ggatcgcgtc agtttgccgc aggcatccag cgagcgcttg atctgccggt aacggcgata
     1801 acccagcagc gccacgagca gcccgatcgg caggaagacc cagcccagcg cctgcacgat
     1861 gaccccctcg agcagcttga tgaacgtcac ccccgcaatg aaaaacgtca gccccgtccg
     1921 cacataggcc agataggtcc gttcgaacgc gagcaccgtc cggtcaatgg ctagctggtc
     1981 gcgcaggatg atctgcgtcg gctcctgatt ctcgcacgga ttgcttccca tggcgccgcg
     2041 ggttcctttg cgtcgagggc ttggcggatc aggccggcgg cctgtcctgt tgaaccgcct
     2101 gccgcaccag ctccaggtcg gcgggcgtat tgatgttgcg aaagctgcgg gcgtcggggt
     2161 cggcgccgcg ccagtcatgc tcggcgacgc ggtcgacgcg caggcgggcc agcgcacgcg
     2221 tcggcgaggc ctcgcccgcc gcgagcaatg cgcgcagtgc gggtagcgcc gcaacgcggt
     2281 agatggcgtg gagcggctgg ggatggtccc gccagatcgg gacgacggcg tcaagctccg
     2341 gcccggcgcg cgccagcaac agccggacga ggcctggttc aatcagcgga caatcacagg
     2401 cgacgacgac ggccagctcg cagcgggcgg cggccagacc cgccacgatg ccgcgcaggg
     2461 ggccctccgg cgcatccggg tcgtccgtca cgcggcgcgc gggccgcgtc agctcaaccg
     2521 cctggccggg ccgggtcacg acgatcagct cgcggcacaa gccccccacg gtccgcgcga
     2581 catgctcgag cagcgtctgg cccgccagga ccgccgcgtc cttgggcgcg ccgtagcggc
     2641 tggagcggcc cccggccagg atgatgccgc tcaacgccgc ttgtgccccc tcggcggggg
     2701 gcgcatcgga actcgcgggc ggaatggatg gaggggtcat ccggtcatta tgcgaagcga
     2761 tggaggcgga tcaaagagaa tcgatggcca agccggagcg agtcgccccc cgccgagccc
     2821 gccagggggg aatggaaaaa cgccggctgt cttgcgtccc ttatgtccct aacgtccctt
     2881 gcccgctcga acgggccgct caaaccgcat ggtgcttcag atcgttgtgc atttcgcgag
     2941 cttgggcgac gatccacaga ctgcggccga tgatgaacag gtagaga
//
LOCUS       DAOPCO010000395         2983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26188_length_2983_cov_4.028347, whole genome shotgun sequence.
ACCESSION   DAOPCO010000395 DAOPCO010000000
VERSION     DAOPCO010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26188_length_2983_cov_4.028347"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PLS90_16845"
     CDS             <1..907
                     /locus_tag="PLS90_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK04115.1"
                     /translation="GFQSCVLLYVNKGSTLHPSFARGVKVEAGGRPIYLPNSNWTDPE
                     TGEIYHWGPQGPSEPNYGWTLPTCVDWDHDGDLDLFVTAQCWRTKYFENIGSRAQPQL
                     ARGRTVRVDGDERAFAWRSKPAAGDLDGDGVAELVGHSARDNIFYCFYPAREQHDASV
                     LELTRGPALLLDSGDPVRGRVGNKNNNGDNHAQLVDWNGDGRLDLLIGSLYGVWQYEN
                     VGTKAQPVFKARGRMQLAGRDLHTFNHAGSFDAADWDGDGRLDLVLGAECPSDQPIGS
                     PLHLFSRYYLEGEFASATVGAFQRR"
     gene            945..>2983
                     /locus_tag="PLS90_16850"
     CDS             945..>2983
                     /locus_tag="PLS90_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK04116.1"
                     /translation="MNRTMAITSLLFLSMLRTVVAQSWTHSTFEDFLAGTAEDGGVNL
                     YVTARGTVSGIYTFDYNRDGANDVLYLNSHDLNDSPFTYIYTNSASGLDQRHLWKLLN
                     DGAYSGTAADLNQDGRTDLVLCGTANGMNRVALDAYIYYGTERGFLLTHLTRLPTLFS
                     YSCAAGDLNNDGWKDLAFAQRGAGILIYWNSEDGFIPDRKTELQTEADYLQLVDVNRD
                     QLLDLVAAGKDRILILLNQPVGFNEDNVEEITAPGVTRFAASDVDQDAHVDLVVAAAA
                     KNSPSPSLIIFGGPDGFDQRPRAHFPTRHAVGCAVADLNSDGHSDIAFADQPTDDSVY
                     ETVNSLIYWGSPTGYDPARKLELPTDWATQCATGDLNGDGWPDLVFANKMSELSLNHS
                     SFVYWGSPDGFDIARRTELPTHGPLDVQISDVNDDQLPDVIFYNATAGHAFSYADLHW
                     NDGKGGFSGDRLTRLTICDGIGSAAADLNLDGYVDLAIANSFEFAPWLKDQGSFVFWG
                     SANGFSDDRKQVLRTKGALCVLTSDLNRDGWLDLIFSEWKNDGNNRIYWGGPEGFSEE
                     NTSALRTNMTVGLGLGDFDKDGWLDIAYGNYGEETAPIYFGSADGFSTASVVRLPSNG
                     SNSVTPADLNRDGWLDLVYSEFNNGKQNDTLSRIYWGAPDGFNPARCTELPTVGAV"
BASE COUNT          580 a          910 c          841 g          652 t
ORIGIN      
        1 cggctttcaa tcctgcgtcc tgctctatgt gaataagggc tctacgctgc atccatcgtt
       61 cgctcgcggt gtaaaggtcg aagccggcgg gcgtccgatt tatctgccca attcgaattg
      121 gaccgacccc gaaaccggtg aaatctacca ttggggcccc cagggaccct ccgaacccaa
      181 ctacggctgg accctcccca cctgtgtcga ttgggatcac gacggcgatc tggacctgtt
      241 cgtcacggcc caatgctggc ggacgaagta ctttgaaaat atcggctccc gagcccaacc
      301 ccagctcgcc cgcggcagaa ccgtccgcgt ggacggcgat gagcgtgcgt tcgcctggcg
      361 ctccaaaccc gcggccggcg atctcgacgg cgatggcgtc gcggagctgg tgggtcattc
      421 cgcccgggat aatatttttt attgctttta tccagctcgc gaacaacacg atgcctccgt
      481 gctggaattg acccgtggac ccgcgctgct ccttgattcc ggcgatcctg tccggggccg
      541 ggttggcaac aagaacaaca acggcgacaa ccacgctcag ttggtcgatt ggaatggcga
      601 cggcagattg gatctgctca ttggcagcct gtatggcgtc tggcagtatg agaatgtcgg
      661 cacgaaagcg cagcccgtct tcaaggcgcg cggccgcatg cagcttgccg ggcgggatct
      721 tcacaccttc aaccatgccg gatcattcga tgccgcggac tgggacgggg acggtcggct
      781 ggatctcgtc ctcggcgcgg aatgcccatc ggatcagccg atcggcagtc cattgcatct
      841 ctttagtcgt tattaccttg aaggcgaatt tgcgtcggcc accgtcggcg cctttcagcg
      901 gagataggtg gcttgccggt atattttgca ggaaaggtaa tgcaatgaat cgaaccatgg
      961 cgataacctc gctccttttt ctctccatgc tgcgaaccgt tgtcgcgcaa tcctggaccc
     1021 actccacttt tgaggatttt ctcgcgggca cggccgagga cggcggcgtt aatctctacg
     1081 tgaccgcccg tggaaccgtg agcggcattt ataccttcga ttacaatcga gacggtgcga
     1141 atgatgtcct gtatctcaac tcgcacgacc tgaacgactc tcccttcacc tacatttata
     1201 ccaattccgc gagcggcctt gatcagcgcc atctctggaa attgctcaac gacggcgcct
     1261 attccggaac cgccgcggac ctcaatcagg acgggcggac cgatctggtc ctctgcggca
     1321 ccgccaacgg gatgaatcgg gtcgcgctcg atgcctacat ttattacggc accgaacgtg
     1381 gtttcctgct gacgcatctc accagactgc ccaccctttt ttcctattcc tgcgcggccg
     1441 gcgatctgaa caacgatggc tggaaggatc tggcctttgc ccaacgcggc gccgggattc
     1501 tgatttactg gaatagcgaa gacggattca tccccgaccg caaaaccgaa ctgcaaacgg
     1561 aggccgatta tcttcagctt gtcgatgtca atcgcgacca gctcttggat ctggtcgccg
     1621 ccggcaagga ccgcatttta attctgttga atcaaccggt tggtttcaat gaagacaatg
     1681 tcgaggaaat caccgcgccc ggcgtcacgc gcttcgccgc ctccgacgtg gaccaggatg
     1741 cacacgtcga tctcgtcgtg gcggcggccg cgaagaattc tccctctccc tcactcatta
     1801 ttttcggcgg acccgatggc ttcgaccaac gcccgcgggc ccacttcccc acgcggcatg
     1861 ccgtcggctg cgctgtcgcg gatcttaact cggatggtca ttccgatatc gcatttgccg
     1921 accaaccgac ggatgacagt gtctacgaga ccgtgaacag cctgatctat tggggttctc
     1981 ctacaggtta tgatcccgcg aggaagttgg aactgcccac cgactgggcc acgcagtgcg
     2041 ccaccggcga cctgaacggt gacggctggc ccgatctggt cttcgccaat aagatgagtg
     2101 agctttctct caatcactca tcgtttgtct actggggatc gcccgatggc ttcgatatcg
     2161 cgcggcgcac cgaactaccc acccatggcc cgctcgacgt gcagatcagc gacgtgaacg
     2221 acgaccagtt gcctgatgtg attttttaca atgccaccgc cggccatgcc ttctcctatg
     2281 cggatttgca ttggaatgac ggcaagggcg gattctcggg cgaccggctg acccggctca
     2341 cgatctgtga cggcattggg tcggccgccg ctgatctcaa cctggacggt tacgtcgatt
     2401 tggcgatcgc caattcgttc gagtttgccc catggctcaa ggatcagggc agctttgttt
     2461 tctggggttc cgcgaacggc ttctccgacg accgtaaaca ggttctgcgc acgaaaggcg
     2521 cgctctgcgt cctgacgagc gatctgaatc gcgacggctg gctcgacctg atcttcagcg
     2581 aatggaaaaa cgacggtaat aaccgcatct actggggtgg cccggagggg ttcagtgagg
     2641 aaaatacctc cgcgctgcgc acgaacatga cggttggcct cggccttggc gatttcgaca
     2701 aggatggctg gctcgacatt gcctatggga attacggcga ggaaacggcc ccgatctact
     2761 tcggatcggc cgacggcttc tccacggcca gcgttgttcg cctgcccagc aacgggagca
     2821 attctgttac tccagccgat ttgaatcgcg acggctggct tgatctggtc tacagtgaat
     2881 ttaataatgg caagcagaat gacactttgt cgcgcattta ttggggcgcg cccgacggat
     2941 tcaatcccgc gcgctgtacc gaactgccga ccgtcggcgc cgt
//
LOCUS       DAOPCO010000396         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26234_length_2978_cov_4.673623, whole genome shotgun sequence.
ACCESSION   DAOPCO010000396 DAOPCO010000000
VERSION     DAOPCO010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26234_length_2978_cov_4.673623"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..2368)
                     /locus_tag="PLS90_16855"
     CDS             complement(332..2368)
                     /locus_tag="PLS90_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04117.1"
                     /translation="MLLTGGSPQVVASPLPEENGFTQFPYFNSRVGSSGMNMEYKFTP
                     EPGVVEMGRRVLELGGDAYKFQLGGDNYRTLYTDLPELPADQRNTTLEVLLNEPAYQA
                     VFDLPLKFFVMYAFGMEMPYYDVIRTGYGNTEWAQKEYDQLYELTRHLLQTYQGTGKV
                     FIITSWESDWPLLEGQGEQGTPTGNRIQGFIEWFNNRYKAINDARNSLPDVTGVEVFH
                     SEEINIIQKVLDDPSKKAIVNAVLPHVTVDLVAYSAYNAINHVEQLSQRLHTHLDYIE
                     SQAKLSGRWHYGRPVIIGEYNYGHLPNGPVRGERSLLALRSAGSWGCPLILYWMTYSG
                     VFTTAWDTGAWIDPDGTRTNEYHQQQEYAGKLHILKNATRVWLNRNPTEAEVNALSAN
                     FHEKSNAEIMREVVNSADFQAALSHRDYLELLFRVCLVTLNFSQTLFTTTLAELESGA
                     ITRWDACLRVIDSSQFAEKVNSADYLQYLAEWQHHFPRPETLIGTRSANFIAAVDGKA
                     FMMRELALRKLDVVDDEIYRRYLPYVDPSIRVGAAPIPQDGIHDLDTTTQAMGLRYFG
                     AAFMDRGVGTVSRLAGAVNDETLMLRDRAAWNSATGLAHAFDNELRFLFRGSGVDVHY
                     LVWDQTDRFHYDLDNGFAQGSMNTWGTPAIKTLRVAAPGSLSPWSRRRWKPGRR"
     gene            complement(2555..>2978)
                     /locus_tag="PLS90_16860"
     CDS             complement(2555..>2978)
                     /locus_tag="PLS90_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04118.1"
                     /translation="LESYRVDYNKYPPQHRDAPPQFPEIMTTPIAYISSVQSALDIFK
                     DKAPNGIAYIEYYWKVYHYWYWKSYFGTFPQTPLELFRASPRVDSFLFYSWGPDQTRD
                     WVEYYGPYGNSVESAPIPPHYDPSNGTVSRGDIITYGP"
BASE COUNT          586 a         1005 c          790 g          597 t
ORIGIN      
        1 cgcacacccc gcatcaatta tcgtcaattt tccccccgcg ttccagtcca ttccctcccc
       61 gttcgtccgc gtccctcgtc cccgtcctcg taatcgcccc cgaaggccct cctcaaaact
      121 cggctcccgg ctctcgttcc tcgtcttcgg gtctcgaaaa ccgatccgac gcgcccacac
      181 ccccccaccc cccaaagcac aagccggctg atgacccgcg ccttgccgac gcggaatcat
      241 caaccggctc actcgcttcg agaccccaat cccatccctg accgctagaa ttccacctcg
      301 atcgcatagg gaaccatctc aggatcgaca gtcagcgtcg tcccggcttc cacctccggc
      361 ggctccatgg cgaaagcgag cccggcgccg ccacgcgcag cgtcttgatc gccggagtgc
      421 cccaggtgtt catcgaaccc tgtgcaaatc cattgtccaa atcataatga aatcgatcgg
      481 tctgatccca gacgagatag tgcacatcga cgccgcttcc ccggaacagg aagcgcagtt
      541 cgttgtcgaa cgcatgcgcg aggcccgtcg ccgagttcca cgccgcgcgg tcgcgcagca
      601 tcagcgtctc gtcattgacg gcgccggcca ggcgcgacac cgtccccacc ccccggtcca
      661 tgaaggccgc gccgaagtac cgcaacccca tcgcctgcgt tgttgtgtcc aggtcgtgga
      721 taccgtcttg cggaatcggc gcggcgccaa cccggatgga tggatcgaca tacggcagat
      781 agcgccggta tatttcatca tccaccacat ccagcttgcg cagcgccagt tcgcgcatca
      841 tgaacgcctt gccgtccacc gcggcgatga aattggccga acgcgtgccg atcagcgtct
      901 ccggccgcgg aaaatggtgc tgccactccg ccagatattg aagataatcc gcgctattga
      961 ctttctcggc gaactgcgac gaatcgatca cccgcaggca tgcgtcccag cgcgtgatcg
     1021 ccccgctttc cagttccgcc agcgtcgtcg taaacagagt ctgactgaaa ttcaatgtaa
     1081 cgaggcagac gcggaagagc agttcgaggt agtcccggtg gctcagcgcg gcctggaaat
     1141 cggcggaatt caccacctcc cgcatgatct ccgcattgct tttctcatga aaattcgcgc
     1201 tcagcgcgtt gacctcggcc tccgtcgggt tccggttaag ccacacccgc gtcgcattct
     1261 tcaaaatgtg cagttttccc gcatattcct gctgctggtg atactcgttc gtccgcgtcc
     1321 cgtccggatc gatccacgcc cccgtatccc acgccgtcgt gaagacgccg gaatacgtca
     1381 tccagtagag gatcagcgga caaccccacg accccgccga ccgcagcgcg agcaggctgc
     1441 gctctccgcg aaccgggcca ttgggcaggt ggccgtaatt atattcacca ataataaccg
     1501 gccggccgta atgccagcga cccgataact tcgcctgcga ctcgatgtaa tcgaggtgcg
     1561 tgtgcagccg ctgggaaagc tgctccacat gattgatcgc gttgtaggcc gagtacgcca
     1621 ccaggtccac cgttacatgc ggcagcactg cattgacaat cgccttcttg ctcggatcat
     1681 ccagcacttt ctggatgatg ttgatttcct ccgaatgaaa aacctcgacg cccgtgacgt
     1741 ccggcagtga attgcgcgcg tcgttgatcg ccttgtagcg gttgttgaac cattcgatga
     1801 acccctggat gcggttcccc gtcggcgtcc cctgctctcc ctgcccttca agcaacggcc
     1861 agtccgattc ccagctcgtg atgatgaaca ccttgcccgt tccctggtag gtctgcagca
     1921 ggtgacgcgt cagctcatag agttggtcgt attccttttg cgcccattcc gtgttgccgt
     1981 accccgtccg gatcacgtcg taatacggca tctccatccc gaaggcatac atcacgaaga
     2041 attttaaggg caggtcgaac acggcctgat acgccggctc attcagcagc acctccagcg
     2101 tcgtgttccg ctggtcggcc ggcagttcgg gaagatccgt gtagagcgtc cggtaattat
     2161 cgccgccgag ctggaacttg taggcgtctc cgcccagctc cagcacgcgc cggcccatct
     2221 cgacgacgcc tggttcgggc gtgaacttgt attccatgtt catccccgaa ctgccgacgc
     2281 gcgaattaaa atacggaaat tgcgtgaagc cgttctcctc gggcaacggc gacgcgacga
     2341 cttgcgggct cccccccgtc agtagcaagg tcgcaacgag aagaagacct gttgaattac
     2401 ggaacatcat gattcagact gcattacctc ccgagacaga catccacgct tcacacattt
     2461 ccggcgcgag gccgggggcg gacccgcgta ggatgcgcga gcccgccccc ttcttcccga
     2521 gcccatcgtc cgccggcccg ccgagcaacc ggtttcacgg gccgtacgta ataatgtcgc
     2581 cccggctgac ggtgccattg gatggatcat agtgcggcgg aattggagcg ctctccaccg
     2641 agttgccata cgggccgtaa tactccaccc agtcgcgggt ctggtccggc ccccacgagt
     2701 agaacaggaa gctgtccacc cgcgggctgg cgcggaacaa ttccaaaggc gtctgcggga
     2761 acgtcccgaa atagctcttc caataccagt aatggtagac cttccaatag tattcgatat
     2821 aggcgatccc attgggggct ttgtccttga agatgtccaa tgccgactgg actgaggaaa
     2881 tgtacgcaat cggcgtcgtc atgatctccg gaaactgcgg cggagcgtcc cggtgctgcg
     2941 gcggatactt gttgtaatcc acacggtacg attccagc
//
LOCUS       DAOPCO010000397         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26286_length_2972_cov_4.471032, whole genome shotgun sequence.
ACCESSION   DAOPCO010000397 DAOPCO010000000
VERSION     DAOPCO010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26286_length_2972_cov_4.471032"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="PLS90_16865"
     CDS             complement(<1..770)
                     /locus_tag="PLS90_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPK04119.1"
                     /translation="MRRISPARSGIERKHNTEMVISHIPLLPRKADQSEQQSPDHSIH
                     QAIELRYRQVHLDFHTSEYIDDVAAQFDAEHFADTLLKAHVDSVTCFARCHHGWLYYD
                     SKLFPERIHPNLCRTDLLREQIEACHRRGIRVPIYVTVQWDAYSGRTHPEWKCIDGNN
                     NRPCGTLDPGFYAYLDVYHPGYRQFLKQHVQELLETLPVDGLFFDIVNPIYSRAPHWI
                     DGMLRLNLNPESEADCLKFAHRVVNEWKLEMTQWVRQF"
     gene            complement(770..>2972)
                     /locus_tag="PLS90_16870"
     CDS             complement(770..>2972)
                     /locus_tag="PLS90_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HPK04120.1"
                     /translation="DSIRVVRYDRETRKVIPASSGGGDYLPCKVERWQRIKRETPGSP
                     QELQPYRVSWLREPDDGDSVYAIYFDLIGHGETEYRAPPSYIGTGDALAFGTSEPQIS
                     TVRGVPIPVDWNGDGRKDLLCAKWTSPGKGVSLRLNLGEMNGEEIFSLPRPIYQPLPG
                     GLLQIDDVNEDGKLDLVCDRGYFTDFVGNRFGQWNHIPDPRAFNVKDVLPQSRSQNYY
                     VVDWDGDSVKDVLISCCVWFEYGWANAFDEKGEWKNGPLDGRFYFFKNLGSNDDFVLD
                     YPLRMTSNDGKPLSVYGYAKFVVQDFDNDGDLDIISSDFLNHIYFFENVGSRSRPEML
                     PAQPILTTQGPFESETQALNVVACDWDDDGDVDLLTRAEDERCALLENLLTDKGELVF
                     KPSRYLQCYDENPVMSQLVAIDLADWDGDGDQDLIAGNSAGYVGIYRNTGADNHLSFA
                     NLEMFKGGTKPIRFQAGYNGSVQGPAEALWGYAVPEVADWNQDGFPDLLCNSIWGLIV
                     WFENPGEIGTLNLTAERPVRVEWPQDPPKPVWRWWDPEPGQWSTQWRSKVQVLDYDDD
                     GLLDAVALDYDGYLVLHRRSKDQNGQLRLGPGERVFLNEDGDPWQINPMKPGSSGRNK
                     FELVDWDGDGDLDLLQSGKDLSITSWHMYPNIRYFENIKLDGTNRFIDRGDLMDVLLA
                     NHTASPAVLDIDQDGVLDLLVGSEDGHIYCYHRSYIEYRDALAAEVVQDAG"
BASE COUNT          639 a          890 c          800 g          643 t
ORIGIN      
        1 ttgaactgcc gaacccattg cgtcatctcg agcttccatt cgttgacgac gcggtgggca
       61 aatttcaggc aatcggcttc cgattccgga ttcaagttaa gccgcagcat gccgtcaatc
      121 caatgcggcg cgcgggaata gatcggattg acaatatcga agaacagccc gtcaaccgga
      181 agcgtctcca gcagttcctg gacgtgttgc ttgaggaact ggcgataccc gggatgatag
      241 acatccagat aggcgtaaaa cccaggatcc aatgtgccgc acggccgatt gttgttgccg
      301 tcgatacatt tccactccgg gtgcgtgcgc ccgctgtatg cgtcccactg caccgttaca
      361 tagattggca cccggattcc ccggcgatgg catgcctcga tttgttccct gagcaggtcc
      421 gtccggcaca ggttcgggtg aattcgctcg ggaaagagct tcgagtcgta atacagccag
      481 ccgtggtggc aacgcgcgaa acacgtgacc gaatcgacat gcgccttcag cagcgtatcg
      541 gcaaagtgct ctgcatcgaa ttgcgcggcg acgtcgtcaa tatactcgct tgtgtggaag
      601 tcaagatgaa cttgccgata gcgtaattca attgcttgat ggattgaatg atccggactc
      661 tgttgctcgg attggtcggc tttgcgcggg agcaggggga tgtgactaat aaccatttcc
      721 gtgttgtgtt tcctctcgat tccacttctc gccggggaga tgcgccgcac tacccggcat
      781 cttgaacgac ctcggccgcc agcgcgtccc ggtattcaat gtaactgcga tggtaacagt
      841 aaatgtgtcc gtcttcgctg ccaacgagca agtcaaggac cccgtcctga tcgatatcga
      901 gcaccgccgg gctggccgtg tggttggcca ggagcacgtc catcaagtcg ccccggtcga
      961 taaacctgtt cgtgccgtca agcttaatat tttcaaaata gcgaatattg ggatacatat
     1021 gccaactggt gatcgaaaga tccttaccgc tctgcagcag gtccaggtcg ccgtcgccgt
     1081 cccaatccac cagttcaaac ttgtttctgc cggacgaccc cggcttcatt gggttgatct
     1141 gccacgggtc gccgtcctca ttcagaaaaa cgcgctcacc cggtcccagc cgcaactgcc
     1201 cgttctgatc cttcgagcgc cggtgcagga ccagatatcc gtcgtaatcg agcgctaccg
     1261 catccagcag gccatcatcg tcgtagtcca ggacctgtac tttggagcgc cattgagtgg
     1321 accattgccc gggctcggga tcccaccatc gccagacggg cttgggggga tcctgcggcc
     1381 attcgacgcg gaccggtcgt tccgctgtca ggttcaaggt gccgatttcg cccggattct
     1441 caaaccagac aatcaagccc caaatgctgt tgcacagcaa gtcgggaaag ccgtcctgat
     1501 tccaatcggc gacttcggga accgcatacc cccacagggc ctcggccggc ccttgcaccg
     1561 acccgttata accggcctga aaacgaatcg gcttagtgcc gcccttgaac atttcgaggt
     1621 tagcgaagga gaggtgattg tccgcgccgg tgtttcgata aataccgaca tagccggcgc
     1681 tgtttccggc aatgaggtcc tgatcgccat cgccgtccca atccgccaga tcaatcgcca
     1741 ccagttggct catcacgggg ttttcgtcat agcattgcaa atatctggat ggcttgaaaa
     1801 ccagttcccc tttgtcggtc aagaggttct ccaggagtgc acagcgctca tcttcggcgc
     1861 gcgtcagcag atcgacgtct ccatcatcat cccaatcgca agcgaccaca ttgagcgcct
     1921 gggtctccga ctcaaacggt ccctgcgtcg tcagaatcgg ttgcgccggc agcatctcgg
     1981 gccggctgcg cgatccgaca ttctcgaaaa agtaaatatg attgaggaaa tcggaggaga
     2041 taatgtccag atcgccgtca ttgtcaaagt cctgaaccac aaacttggcg tagccataaa
     2101 cgctcaacgg cttgccgtcg ttggacgtca tgcgcagcgg ataatccagt acaaaatcat
     2161 cgttcgagcc aaggttcttg aagaagtaga accgcccgtc caacggaccg ttcttccatt
     2221 cgcccttttc gtcaaaggca ttggcccatc catactcgaa ccagacacaa caactgatca
     2281 gcacgtcctt gaccgaatcg ccatcccaat cgacgacata gtagttttgc gatcgcgatt
     2341 gcggcaggac gtccttcaca ttgaacgcgc gcggatcggg aatatgattc cattggccga
     2401 atcgatttcc gacaaagtcg gtaaaatagc cgcggtcgca aaccagatcg agcttgccat
     2461 cttcattgac gtcatcaatc tggagcaacc cgcccggcag cggctgataa atcggccggg
     2521 gcaaggaaaa aatctcttcg ccgttcatct cgccgagatt cagccgaagc gacacaccct
     2581 tgcccggcga cgtccatttg gcgcagagca ggtccttccg cccgtcgccg ttccagtcca
     2641 ccgggatcgg cacgccccgc acggtactga tttggggttc gcttgtgcca aaggcgagcg
     2701 cgtctcccgt cccgatatag ctgggagggg cgcgatactc cgtctcgccg tgtccaatca
     2761 ggtcgaaata gatggcgtag acgctatcgc cgtcgtcggg ttcgcgaagc caactgacgc
     2821 ggtagggttg aagttcctgt ggacttccag gagtttcacg cttgatgcgc tgccaccgct
     2881 cgaccttgca cggcagatag tcgccgccgc cgcttgaagc cggaatgacc ttccgggttt
     2941 cccgatcata gcgcacgacc cgaatcgagt cg
//
LOCUS       DAOPCO010000398         2967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26341_length_2967_cov_4.116071, whole genome shotgun sequence.
ACCESSION   DAOPCO010000398 DAOPCO010000000
VERSION     DAOPCO010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26341_length_2967_cov_4.116071"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLS90_16875"
     CDS             <1..358
                     /locus_tag="PLS90_16875"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HPK04121.1"
                     /translation="LPIKVWLMRQDRLVGLGNIYVSEALHRAGIHPARPARSLSFDEA
                     ARLHKALRLILRSAIRCGGTTIFNFENAVGAAGQFQRRLRVYGREGDPCARCRTPIQR
                     LVQQQRSTFFCPTCQH"
     gene            670..756
                     /locus_tag="PLS90_16880"
     tRNA            670..756
                     /locus_tag="PLS90_16880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:704..706,aa:Leu,seq:cag)
     gene            1067..>2967
                     /locus_tag="PLS90_16885"
     CDS             1067..>2967
                     /locus_tag="PLS90_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04122.1"
                     /translation="MWLKWLPWKFIVRRMARSKGFLDPIALLSYVRRFSQPSEVHEPI
                     ELLRAGVVLHARGLMNSRSIQQNLDWVWPMWVERQFNPHDVAFVPRAFSLTHINLTHR
                     NWTAVGIPDYEAYPLVDPHGLVIPHYDGWSLDAWIVTREGRLIAPSKLPAVEQELNYE
                     PNLAVVTRAEEDGFQIHSRVEMTLVDGQPVCRIAVAGRAPCDAWLVISARPYNPEGID
                     FIHEIELTGNRQGWLIDGKDEVRFSAPADLHSFSEYHAGDVSMHLTRPRDARRVVCNV
                     GMATAAVLFQLAPETPRAITCDVPVRPENAPPLPPPPSAEQAWADSLAHACRLRHPDP
                     QIQFLWDVHLRTLVQHAPGDVYPGPYTYKRFWFRDAAFMLHALACVGLDQRVERVLDR
                     FPERQTPFGYFLSQEGEWDSNGEALWIMQRYCALTGRPPKRGWRTAIERGAHWIDRKR
                     SREGESDPVHQGLFPPGFSAEHLGPNDYYYWDDFWGVAGLMAAAELMLAYGDEPQARQ
                     FERYALQFMQAIEHSLRLAAERLGRPAMPAAPDRRLDPGMIGCLVVGYPLQLWRGDDH
                     RLLDTLDYLMSQCRVHGGFFQDMIHSGINPYLTLHMAQVLLRAGDGRYLELMKAVARL
                     ASPTGQWPEA"
BASE COUNT          438 a         1167 c          835 g          527 t
ORIGIN      
        1 tttacctatc aaggtctggc tgatgcgcca ggatcggctc gtcggcctcg gcaatattta
       61 cgtctccgag gcgctccatc gcgccggcat ccatccggcg cggccggccc gctcgctctc
      121 cttcgacgaa gcggcgcgcc tgcacaaggc cctccgcctc atcctgcgca gcgccatccg
      181 ctgcggcggc accaccatct tcaacttcga aaatgccgtg ggcgccgccg gccagttcca
      241 gcgccggctc agggtctacg gccgcgaggg cgacccctgc gcccgctgcc gcacgccgat
      301 ccagcgcctc gtccagcaac agcgcagcac cttcttctgc cccacctgcc agcactagga
      361 tcgtccctcg tccctgtcct cgtggtcgct ctcgaagggc tgcccatttt cctcccccgc
      421 gtcgtgaacc gccggttcat gaccaactca cgtccctgct gtcccttatg tcccttgcgt
      481 cctttccgtc cctgcccttg gcgcaagcgc ccgccatttt tttgcctgat tccccccttg
      541 cattggcctg gccgacccgc tataagttgg gctgggtatg ggctgaacag acctgacgtg
      601 cggttgcacc gccgcggcgg cgacctccgc cggttcagcc ccacgcggtc cgcccggatc
      661 gcgccaggtg ccgaagtggc ggaattggca gacgcgcacg gttcaggtcc gtgtgggggt
      721 gaccccgtgg aggttcgagt cctctcttcg gcaccatctc tcccctattc gtccctcatc
      781 ttcgtcctcg ccctcagcgg ccccccataa ttcggctctc ggctctcgtt cctcgtcctc
      841 ggctctcgaa aaccggccat tttgtcccct cgttgcgacc accgggttca tgagcaactc
      901 atcatccgcc ccaacggact ctaccccgca ttcccgcccg ccaaatgcca tcgtgaaccc
      961 ttcacgcccg tcatgctaaa ctgattttcg cggatccatg gcccggtgat tgcttcccgc
     1021 ccagggggga cacgaccggg cgcacccgcg cggggaaggt caaggcatgt ggctgaaatg
     1081 gttaccgtgg aaattcatcg tgcgccgcat ggcgcgctcc aagggcttcc tcgacccgat
     1141 cgccctgctc agctacgtcc gccgcttctc ccagccctcc gaagttcacg agccgattga
     1201 gctcctccgc gccggcgtcg tcctccatgc ccgcggtctg atgaacagcc gctcgatcca
     1261 gcagaacctg gattgggtct ggcccatgtg ggtcgagcgc cagttcaatc cgcacgacgt
     1321 cgccttcgtc ccccgcgcct tctccctcac ccacatcaac ctgacgcatc gcaactggac
     1381 cgccgtcggc atccccgact acgaagccta cccgctggtc gatccacacg gcctggtcat
     1441 cccgcactac gacggctggt cgctcgatgc gtggatcgtc acgcgcgagg ggcgcctgat
     1501 cgccccctcg aaactccccg ccgtcgagca ggaactgaac tatgaaccca atctcgccgt
     1561 cgtgacccgg gcggaggagg atggcttcca gatccactcc cgcgtcgaga tgacgctcgt
     1621 cgacggccaa ccggtctgcc gcatcgcggt cgcgggccgc gccccctgcg atgcctggct
     1681 cgtcatctcg gcccgcccct acaacccgga agggatcgat ttcatccacg agatcgagct
     1741 gaccggcaac cgccagggct ggctgatcga cggcaaggac gaggtccgct tcagcgcgcc
     1801 ggccgacctg cactcgttct ccgaatacca tgccggcgat gtctcgatgc acctgacccg
     1861 gccgcgcgat gcgcgccgcg tcgtctgcaa tgtcggcatg gcgacggcgg ccgtcctgtt
     1921 ccagctcgcg ccggagaccc cccgcgccat cacctgcgac gtccccgtcc ggccggaaaa
     1981 cgctccgcca ctgccgccgc ccccctcggc cgaacaggcc tgggccgaca gcctggcgca
     2041 cgcctgccgc ctgcgccacc ccgaccccca gatccagttc ctctgggacg ttcacctgcg
     2101 cacgctcgtc cagcacgccc ccggcgacgt ctaccccggc ccatacacct acaagcggtt
     2161 ctggttccgc gacgccgcgt tcatgctcca cgccctcgcc tgcgtcggcc tcgaccagcg
     2221 cgtcgagcgc gtgctggatc gtttcccgga acgccagacc ccgttcggct acttcctctc
     2281 tcaggagggc gaatgggact ccaatggcga agcgctctgg atcatgcagc gctactgcgc
     2341 gctgaccggg cgcccgccga aacgggggtg gcgcaccgcc atcgaacgcg gcgcccactg
     2401 gatcgatcgc aagcgcagcc gcgagggcga atccgacccc gtccaccagg gcctcttccc
     2461 gcccggcttc agcgccgagc acctcgggcc gaacgattat tactactggg acgatttctg
     2521 gggcgtggcc ggcctgatgg ccgccgccga actgatgctc gcctacggcg acgaacctca
     2581 ggcccgccag ttcgaacgct acgcgctcca gttcatgcag gcgatcgagc acagcctgcg
     2641 cctcgccgcc gaacgcctcg gccgccccgc catgcccgcc gcccccgacc gccgcctcga
     2701 tcccggcatg atcggctgcc tcgtcgtcgg ctaccccctc cagttgtggc gcggcgacga
     2761 ccatcgcctg ctcgacaccc tggactatct gatgagccag tgccgcgtcc acggcggctt
     2821 ctttcaggac atgattcatt cggggatcaa cccctacctg acgctccaca tggcccaggt
     2881 attgctccgc gccggcgatg gccgctatct ggaattgatg aaggccgtgg cgcgcctcgc
     2941 ctcgccgacc ggccagtggc ccgaggc
//
LOCUS       DAOPCO010000399         2957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26439_length_2957_cov_6.049966, whole genome shotgun sequence.
ACCESSION   DAOPCO010000399 DAOPCO010000000
VERSION     DAOPCO010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26439_length_2957_cov_6.049966"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..2562)
                     /locus_tag="PLS90_16890"
     CDS             complement(142..2562)
                     /locus_tag="PLS90_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04123.1"
                     /translation="MVLSLALCLWASASASANPVWIEDFKQDPFGMDHKWKQPQIAMG
                     GGVAEFRSDGEVGRLLFRPDKSFRTTVELAHDIAWNPAENRYIQLRVSRLSPEARLKL
                     YVMSDADARRPQRVLNFRNQESVLPPTVLTVDLHDQLSPETRSIGLMLELDRDNRQQV
                     PGQVEVYIDWIKAGMSGDAEVPGTPRIAVSSEQGQAGAARRLEWTAPADYAGDLYTLS
                     LSRDPLFAGACTTLLDTVSGTSYTLPDDLPPGKWYWTVSATAADGRTGEFLTGETAQE
                     PPFIEIAGSDEETAGTFPYFSVRAGSQGFGCIEKFTDDPLIVEQAKLMLEMGSDAYKF
                     LFGSDMEANNYTKCYTDMSTETLHASHTLVELIEKEPAYKAVFDMPFKYFVMWTYAMD
                     LDYAVLGTGEYTGEVVKEEYDQLYELTRYLMKTYAGTGKIFLIGHWEGDNMLMQGKDG
                     MTASPSQTMIDDMRAWYINRQQAISDARASLPDVTGVEVYHYAEMNAVSPVLDKGLPR
                     LINAVLPDVPVDLVSYSAYNCMNHTDELPDRAYLHLDYILEHAAFTGAWKHGKPVFIG
                     EYGIPMPLVENRPARNLIALKSATSWGSLINLYWSVYTQLPNGNSSLIELDGQPNQEY
                     RYFEDFTAKMHILKHATRVWLGRNPTEIEANWFATEFDQLARHEVLACVIDSLEHRVT
                     VDDVTWLRRLLERCGIAGSDAEALAGELLDPLGREELTRFGALLRVFDSDAFARAVSN
                     EQFDAYLTGFMMPDGAEAPAWPAELKSRSERYLWVFDSDAFRRKNLQFANENRVTDAI
                     RAMYQPAF"
BASE COUNT          534 a          930 c          921 g          572 t
ORIGIN      
        1 agtttgcggg gaacaggagg caggtgcaat cagaatcggc gtccccggcc gcgaaaacgg
       61 gtccggtggg gaaccaggca ggcgcgaccc gctgggcaag ccatcgccga attcgatcag
      121 gcgcaagccc cgccctgttt ctcagaatgc cggctgatac atcgcgcgga tggcgtcggt
      181 cacccggttc tcattggcga attgcaggtt cttccggcgg aaggcatcgc tgtcgaaaac
      241 ccacaggtaa cgctccgacc ggctcttgag ttcggccggc caggccggcg cctccgcgcc
      301 gtccggcatc atgaacccgg tcaggtaggc gtcaaattgt tcgttcgaga cggccctggc
      361 gaacgcgtcg gagtcgaaaa cgcgcagcag cgcgccgaac cgggtcagct cctcccgccc
      421 caacgggtca agcagttcgc ccgccagcgc ctcggcgtcg gacccggcaa tgccgcaccg
      481 ctcgagcaac cggcgcagcc aggtcacgtc gtccaccgtc acccgatgct cgagcgaatc
      541 gatcacgcag gcgagcactt catggcgcgc gagctggtcg aactccgtgg cgaaccagtt
      601 cgcttcgatt tcggtcggat tgcgcccgag ccagacgcgc gtggcatgct tcaggatgtg
      661 catcttcgcc gtgaaatcct cgaaataccg gtattcctgg ttgggctggc catccagttc
      721 gatcaacgag gaattgccgt tgggcaactg tgtgtagacc gaccagtaga gattaatcag
      781 gctgccccaa ctggtcgcgg acttgagcgc gatcagattg cgcgccggcc ggttctccac
      841 gagcggcatc gggatgccgt actcaccgat aaagaccggc ttgccgtgct tccatgcgcc
      901 ggtgaaggcg gcgtgctcca ggatgtagtc cagatgcagg tacgcccggt cgggcagttc
      961 gtcggtgtgg ttcatgcagt tgtaggccga atacgagacc agatcgacgg gcacatcggg
     1021 caggacggcg ttgatcaggc gcggcagtcc cttgtcgagg accggggaaa ccgcattcat
     1081 ttcggcataa tgatagactt ccacgccggt cacgtcgggc agcgaggcgc gcgcgtcgga
     1141 gatcgcctgc tggcgattga tataccacgc gcgcatgtcg tcgatcatcg tctggctcgg
     1201 cgaggcggtc atgccgtcct tcccctgcat cagcatgttg tcgccttccc aatggccaat
     1261 gagaaagatc ttgcccgtcc ccgcataggt tttcatgaga taccgcgtca gctcgtaaag
     1321 ctggtcgtat tcctccttca cgacctcccc cgtgtattcc cccgttccca ggacggcgta
     1381 gtcaagatcc atggcgtagg tccacatgac gaaatacttg aagggcatgt cgaagacggc
     1441 cttgtaggcc ggttccttct caatcagctc gaccagcgtg tggctcgcgt gcagtgtctc
     1501 ggtcgacatg tcggtatagc atttggtgta gttgttcgcc tccatgtcgc tgccgaacag
     1561 gaatttatac gcgtcgctgc ccatctcgag catcagtttg gcctgttcca cgatgagggg
     1621 atcgtcggtg aatttctcga tgcagccgaa gccctggctg ccggcgcgga cgctgaaata
     1681 ggggaacgtc ccggccgtct cctcgtcgga cccggcaatt tcaatgaacg gcggctcctg
     1741 cgcggtttca cccgttagaa attcgccggt gcgtccgtcg gccgccgttg cgctcacggt
     1801 ccagtaccat ttcccgggcg gcagatcgtc cggcagcgtg tagcttgtcc cgctcaccgt
     1861 gtcgagcagg gtggtgcagg cgccggcgaa cagcggatcg cgcgacaggc tcagcgtata
     1921 gaggtcgccg gcgtagtcgg cgggcgccgt ccattccagg cggcgcgccg cgccggcctg
     1981 cccttgctcc gaggaaacgg cgattcgcgg cgtccccgga acttccgcgt cgccggacat
     2041 cccggcctta atccaatcga tatacacttc cacctgaccg ggaacttgct gccgattatc
     2101 ccggtccaac tccagcatca ggccgatgga ccgcgtctcg ggggaaagct ggtcgtgaag
     2161 atcgaccgtc agcacggtcg gcggcaggac ggattcctga ttcctgaaat tgagcacgcg
     2221 ttgcggccgc cgcgcgtcgg cgtcgctcat gacgtagagt ttcagtctgg cctcgggcga
     2281 gaggcggctg acccgaagct gaatgtagcg gttttccgcg ggattccagg cgatatcgtg
     2341 ggccagttcc accgtcgtgc ggaacgattt gtcgggccgg aagagaaggc ggccgacttc
     2401 gccgtcggag cggaactcgg ccacgccgcc ccccatggcg atctggggtt gcttccattt
     2461 gtgatccatg ccgaaggggt cctgcttgaa gtcctcgatc cacaccggat tggccgacgc
     2521 gctcgccgaa gcccacaggc agagcgccag cgacagaacc attcctgtca accgacgcct
     2581 gacgcaaacc ggggatcggt ccgttttcat tagaatttcc gtgctggtcg tcggcatgat
     2641 ggcggtgact ccttttcgtg acaatcgcct ggcttcaaaa tatcggtgcc tccacacaag
     2701 cggcaggaac ccgaccggct cgcgtcgatc cgcatttaat gctgatcact ttgggaggca
     2761 ggaacatccg ggagccacga gacgaaagga gcgggatgga cgcatggcca cagggcgccg
     2821 gccgcgtcaa tccattgcac accttctcgt ttctttcatg acccagaatt ccggaaatcc
     2881 cccgacccgc gggggattca catccggcgc tggatcgcca gcttatcgtt ccgcgtccgc
     2941 cggcgcgtgc agcagcg
//
LOCUS       DAOPCO010000400         2951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26504_length_2951_cov_4.533494, whole genome shotgun sequence.
ACCESSION   DAOPCO010000400 DAOPCO010000000
VERSION     DAOPCO010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26504_length_2951_cov_4.533494"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLS90_16895"
     CDS             <1..505
                     /locus_tag="PLS90_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPK04124.1"
                     /translation="GLETHTMSKGWDMIGWRLGFVAGHPRLVAAYADMKDNSDSGQFI
                     AIQKAGVAALDDAALLPAIRAKYERRLRKLVDALRELGFDANMPGGTYFLMVKAPRGV
                     EGGPRFDDAEAASQWLIREKMVCTVPYAEGEMLRFSTTYRVDGEAGEDALMAELNQRL
                     AGIRFLF"
     gene            694..>2951
                     /locus_tag="PLS90_16900"
     CDS             694..>2951
                     /locus_tag="PLS90_16900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK04125.1"
                     /translation="MWRPLRILILTPDRALAAELARLLAPLDDANECIPVSTADGLPE
                     TLADGPCAAAILDDAWGEDGLLRALNRLQSLHPMPPCIVLHRRLSLAGVVAAVKAGAA
                     DVIPHDDLPRAVAALHLALRPAERPPRPQAAQPEPCLSEATLRSLLERMPELVIVLDE
                     AARVLEVFTADPSLLWRPAAEIIGKSLPETAPPALAALVCAGLRRALESAQVQHLDDW
                     FELDGERRWFSSRLLALDGPGPTRLLWVARDLTPEKQTEEALRRGIARYETLARIAPV
                     GILLMDPEGRLQYVNDRLCQLAGRPQEALVGNHWRAVIHPEDAGWLEPQVQRSLERDL
                     PLRTECRLVQPGGDIRWVVVQTMIERDQAGNSVGYVGTITDITDRRKAEEHREHLEAQ
                     LRQSQKMESIGQLAGGIAHDFNNLLQAILGYTDMAMLGLDERDQRRLDLLEVRHAAER
                     ATMLTQQLLAFSRRQILRMSDIALNDLIQNLLLMLRPLLGEHIQVDFIAGRSLGTVRA
                     DAGQLEQVILNLCVNARDAMPDGGRLTIQTENVRLDGGDLPDHPWARPGHYVLLSVSD
                     TGCGIAPDHLEHIFEPFFTTKEVGQGTGLGLAMVHGIINQHGGIVRADSAAGIGTTLK
                     IYLPVAERRAAADGPRLADSISGGSETILVAEDEPRVRGLVRRLLEGAGYRVLLAADG
                     REAVEMARSHAGEFQLTILDVVMPRMNGREVREAIRRNNPAMKFLFSSGYSAGEGPTD
                     FLIGDEAVLIQKP"
BASE COUNT          470 a         1142 c          875 g          464 t
ORIGIN      
        1 cggccttgaa acccacacga tgagcaaggg ctgggacatg atcggctggc gcctgggctt
       61 cgtcgccggc catccccggc tggtcgccgc ctacgccgac atgaaggaca actccgactc
      121 cggccagttc atcgcgatcc agaaggccgg cgtcgcggcg ctcgacgatg ccgccctgct
      181 gccggcgatc cgcgccaaat acgagcgccg cctgcgcaag ctcgtcgatg cgctccgcga
      241 actgggcttc gatgccaata tgcccggcgg cacctatttc ctgatggtca aggccccccg
      301 cggtgtcgag ggcggacccc gcttcgacga tgccgaagcc gccagccagt ggctcatccg
      361 cgaaaaaatg gtctgcaccg tcccttacgc cgagggcgaa atgctgcgct tctccaccac
      421 ctatcgcgtc gacggcgagg cgggcgaaga cgctctgatg gccgaactga accaacgcct
      481 ggccggcatc cgcttcctgt tctgagccgc cccgccgcgc ccgctccggc ccggcttgcg
      541 aaatccgctt tcttttcctg aggggacggt tattctcttg ctcgacgtcc agggtcgtca
      601 ggcgtcctcc ccccgccacg cctggcgccg accggcgtcc ggttctcgtc cgttgtccgt
      661 tcgattcacc gcattgggat ctcgttcaaa accatgtggc gcccactccg catcctcatt
      721 ctgacgcccg atcgcgccct cgccgccgag ctcgcccgcc tgctggcgcc cctcgacgac
      781 gcgaacgagt gcatcccggt ttccaccgcg gacggcttgc ccgaaaccct ggcggacggc
      841 ccgtgcgctg cggcgatcct cgacgacgcc tggggcgagg acggtctgct ccgcgcgctc
      901 aaccggctcc aatcgctcca tccgatgccg ccctgcatcg tcctccatcg gcgcttgtcg
      961 ctggccggcg ttgtggcggc cgtcaaggcg ggcgccgccg acgtcatccc ccatgatgat
     1021 ttgccgcgcg cggtcgccgc gctccatctg gcgctgcgcc ccgccgaacg cccgccccga
     1081 ccgcaagccg cccagccgga accttgcctc tccgaggcaa ccctccggtc gctcctggaa
     1141 cggatgcccg agcttgtcat tgtattggat gaggcggcgc gcgtcctcga ggtcttcacc
     1201 gccgatccct cgctcctctg gcggcccgcc gcggagatca tcggcaagtc cctgccggaa
     1261 acggcgccgc cggcccttgc cgcgctcgtc tgcgccggcc tgcgccgcgc gctcgagagc
     1321 gctcaggtcc agcatctgga tgactggttc gagctcgacg gcgagcgccg ctggttctcc
     1381 agccgcctgc tcgcgctcga cggccccggg cccactcgcc tgctgtgggt ggcgcgcgat
     1441 ctgacgccgg aaaagcagac cgaggaagcc ctccgccgcg gcatcgcgcg ctacgaaacc
     1501 ctcgcgcgca tcgcccccgt cggcatcctg ctaatggatc ccgaaggccg cctgcaatac
     1561 gtcaacgacc ggctctgcca gctcgccggc cgcccgcaag aggcgcttgt cgggaatcat
     1621 tggcgcgccg tgatccaccc cgaggacgcc ggctggctcg agccccaggt ccagcgctcg
     1681 cttgagcgcg atctgcccct ccgaaccgaa tgccgcctcg tccagcccgg cggcgacatc
     1741 cgctgggtcg tcgtccagac gatgatcgag cgcgaccagg ccggcaacag cgtcggctat
     1801 gtcggcacaa tcaccgacat caccgaccgg cgcaaggccg aggaacatcg cgaacacctc
     1861 gaagcgcaac tccgccagtc gcagaaaatg gaaagcatcg gccagctcgc cggcgggatc
     1921 gcgcacgatt tcaacaacct gctccaggca atcctcggct acaccgacat ggccatgctc
     1981 ggcctggatg aacgcgatca gcggcggctc gatctgctcg aggtccgcca cgccgccgaa
     2041 cgcgccacca tgctcacgca acagttgctc gccttcagcc gccgccagat cctgcgcatg
     2101 agcgacatcg cgctcaatga cctgattcag aacctcctcc tgatgctgcg cccgttgctc
     2161 ggcgagcaca ttcaggtcga tttcatcgcc ggccggagcc tgggcaccgt ccgcgccgac
     2221 gccggccaac tcgaacaggt catcctcaac ctctgcgtca atgcgcgcga cgccatgccc
     2281 gatggcggcc gcctcacgat ccagaccgaa aatgtccgcc tcgacggcgg cgacctgccc
     2341 gatcacccct gggcccggcc cggacactat gtgctcctct cggtcagcga caccggttgc
     2401 ggcatcgccc ccgatcacct cgaacacatc ttcgaaccat tcttcactac caaggaggtc
     2461 ggccagggaa ccgggctcgg tctggccatg gtccacggca tcatcaatca gcacggcggc
     2521 atcgtgcggg ccgacagcgc ggcgggcatc ggcaccacgc tcaagatcta cctccccgtc
     2581 gccgagcgcc gcgccgccgc cgatggaccg cgtctcgccg attccatttc cggcggcagc
     2641 gaaaccattc tcgtcgccga ggacgaaccg cgcgtccgcg gactcgtccg ccgcctcctc
     2701 gagggcgccg gctaccgggt cctgctggcc gccgacggcc gcgaggccgt cgagatggcc
     2761 cgcagccacg ccggcgaatt ccagctcacg atcctcgacg tcgtcatgcc gcgcatgaac
     2821 ggccgcgaag tccgcgaggc gatccgaaga aataaccccg ccatgaagtt cctcttctcc
     2881 agcggctaca gcgccggcga aggccccacc gatttcctca tcggcgacga agcggtcctg
     2941 atccagaaac c
//
LOCUS       DAOPCO010000401         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26634_length_2941_cov_3.788981, whole genome shotgun sequence.
ACCESSION   DAOPCO010000401 DAOPCO010000000
VERSION     DAOPCO010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26634_length_2941_cov_3.788981"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLS90_16905"
     CDS             complement(<1..537)
                     /locus_tag="PLS90_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006912198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPK04126.1"
                     /translation="MSSAPLNPPPPRDAQPHGPLAWLVHNRVTPNLMMLVLLVGGVLT
                     ALSIKQEVFPEFSADQISIRVAYPGAGPEEVENGIILVIEEAVRAIEGIVEMQSTARE
                     GSADVMLEIEPNGDHQKIYTDVQQAIARIRTFPDDAEEPQIELVTRRRDVLEVQLYGD
                     VDEFTLRNIAEEVRDRLLQ"
     gene            complement(530..1825)
                     /locus_tag="PLS90_16910"
     CDS             complement(530..1825)
                     /locus_tag="PLS90_16910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPK04127.1"
                     /translation="MRPPHDPPPRGGTFARRLLRVALKLLFGLLLPVAVLLVSGLMAA
                     RLMQTAPEAERGGPRKREGRLVETLTLHAVDRTVTVHAQGVVVPARRVTLFPQVSGKL
                     QYVDDALIPGARIREGQKLFAIDDRDYQLAVRQRLAELQQAQSALKLEEGQQQIARQE
                     YELLGEQLDEEDAAWVLRRPQLENAQAAISLAEAALDAARLQLARTEITAPFDSIVLQ
                     KHVDRGTMVGPSTPLVELAGTQQFWVELSVPLDDLRWIRMPGPDGQPGAEVRLFQELT
                     WDPARFRMGRVIRLAGEINPASRLARLIVAVDDPLALAAEHDGEPPLLLNSYVTAEIA
                     GQPLQNVIPISREFVRNGEYAWIMDEAGLLQIRLLDIAFRGKEEVLVTGGVRDGEQLV
                     TTKLDSPVQGMLLRTREQHPEAAPAAEPLRPAVQDAADE"
     gene            complement(1890..>2941)
                     /locus_tag="PLS90_16915"
     CDS             complement(1890..>2941)
                     /locus_tag="PLS90_16915"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPK04128.1"
                     /translation="MYRQLYERRRRLAVSPAEVALAWYDLRAQQARLALLDEQTETAR
                     IILELIEARFRLGQARATDVLQQRQLVESLLGAREQVLAVVGLLQNQLAVLLGDVPGQ
                     VAFDAPPDLIDLPPLPQTGIPAEWVRARPDVRAAYRDLQAADQQLAAAIADRFPRVTL
                     AAALEAASPDWNAVHDNWVATLAANLVAPIMDGVRRRAEIDRRRALVAERLEQYAQAI
                     LLAVREVEDALTREARQRAFLASLERQIETAGQVLERTRFEYLRGAQDYLRVLDAQQT
                     LYDLQADRIAARSQLIEHRIQLCRALAGGWRLEPPDALQTVESNQRDNPPPPPTPTWE
                     RVPLPPPDPPPAAGA"
BASE COUNT          411 a          808 c         1255 g          467 t
ORIGIN      
        1 ctggaggaga cggtcgcgga cctcctcggc gatgttgcgc agggtgaact cgtcgacatc
       61 gccgtagagc tggacctcga ggacatcgcg gcggcgggtg accaattcga tctggggttc
      121 ctcggcgtcg tcggggaagg tgcggatgcg ggcgatggcc tgctggacgt cggtgtagat
      181 tttctggtgg tcgccgttgg gttcgatttc gagcatcacg tcggcggaac cctcgcgggc
      241 ggtcgattgc atttcgacga tgccctcgat cgcgcggacg gcttcctcga tgacgaggat
      301 gatgccgttt tcgacttcct cagggccggc gccgggatag gcgacgcgga tcgagatctg
      361 gtcggcgctg aactcgggga agacttcctg cttgatggaa agggcggtga ggacgccgcc
      421 gacgagcagg acgagcatca tcagattggg cgtgacgcga ttgtggacaa gccaggcgag
      481 ggggccgtgg ggctgggcgt cgcgcggcgg gggcgggttg agcggggcgc tactcatcgg
      541 cggcatcctg gacggcgggg cgcagcggtt cggcggcggg cgcggcctcg gggtgctgct
      601 cgcgggtgcg cagcagcatg ccctggacgg gggaatccag cttggtggtg acgagctgct
      661 cgccgtcgcg gacgccgccg gtgacgagga cttcctcctt gccgcggaag gcgatgtcca
      721 gcaggcggat ctggagcagg ccagcctcat ccatgatcca ggcgtattcg ccgttgcgga
      781 cgaactcgcg gctgatgggg atgacgttct ggaggggctg gccggcgatc tcggccgtga
      841 cgtagctgtt gaggagaagc gggggctcgc cgtcgtgctc ggcggcaagg gcgagggggt
      901 catcgacggc gacaatcagg cgggcgaggc ggctggcggg gttgatttcg ccggccaggc
      961 ggatcacccg gcccatgcgg aagcgggcgg ggtcccaggt caattcctgg aacaggcgga
     1021 cctcggcgcc gggttggccg tcggggccgg gcatgcggat ccagcgcagg tcgtcgaggg
     1081 ggacggagag ttcgacccag aactgttggg tgccggcgag ctcgacgagc ggggtggagg
     1141 ggccgaccat cgtgccgcgg tcgacgtgtt tctggaggac gatggaatcg aagggggcgg
     1201 tgatttcggt gcgggcgagc tggaggcggg cggcatcgag cgcggcctcg gccaggctga
     1261 tcgcggcctg ggcgttttca agctgaggcc ggcgcaggac ccaggcggcg tcctcttcat
     1321 cgagctgctc gccgagcagt tcgtattcct ggcgggcgat ctgctgctgg ccttcctcga
     1381 gcttgagggc gctctgggcc tgctggagct cggcgaggcg ctggcggacg gcgagctgat
     1441 agtcgcggtc gtcgatggcg aagagtttct ggccttcgcg gatgcgagcg ccggggatca
     1501 gcgcatcgtc gacgtattgg agtttgccgc tgacctgcgg gaacagggtg acgcggcggg
     1561 cgggaacgac gacgccctgg gcgtggacgg tgacggtgcg gtcgaccgcg tggagggtga
     1621 gggtttcgac cagtcgccct tcgcgtttgc gcgggccgcc gcgctcggct tcgggagcgg
     1681 tctgcatcag gcgcgccgcc atcaggccgg agacgagcag gaccgccacc ggcaggagca
     1741 ggccgaagag gagcttgagc gcgacgcgca gcagccggcg ggcgaaggtt cctccgcggg
     1801 gaggtgggtc atggggcggt cgcatggttg gggtgagttg gcctcgtggg ggaatcggtt
     1861 aagggttatt ggagattcgt tggcgcgtgt caggcgccgg cggccggcgg tggatcgggc
     1921 ggaggcaggg gcacccgctc ccaggtcggg gtcggcggcg gcggcgggtt gtcccgctgg
     1981 ttggactcga cggtttggag cgcatcggga ggttcaaggc gccagccgcc ggcgagggcg
     2041 cggcagagct gaatgcggtg ctcgatgagt tggctgcgcg cggcgatgcg atcggcctgc
     2101 aaatcataga gggtctgctg ggcgtcgagg acgcggaggt aatcctgggc gccgcggagg
     2161 tattcgaagc gggtgcgctc gaggacctgg ccggcggttt cgatctggcg ctcgagactg
     2221 gcaaggaagg cgcgctggcg ggcctcgcgg gtgagggcgt cctcgacttc gcggacggcg
     2281 aggaggatcg cctgggcata ttgttcgagg cgctcggcga cgagggcgcg ccggcggtcg
     2341 atctcggcgc ggcggcggac gccgtccatg atcggcgcga cgaggttggc ggcgagcgtg
     2401 gcgacccagt tatcgtgcac ggcattccag tcgggggagg cggcctcgag ggcggcggcg
     2461 agggtaacgc gcgggaagcg gtcggcgatg gcggcggcca gttgctgatc ggcggcctgg
     2521 agatcgcgat aagcggcgcg cacgtcgggc cgggcgcgga cccattcggc cgggatgccg
     2581 gtctggggga ggggcggcag atcgatcaga tcgggcgggg cgtcgaaggc gacctggccg
     2641 gggacgtcgc cgaggaggac ggcgagctga ttctggagga ggccgacgac ggccaggacc
     2701 tgttcgcggg cgccaaggag cgactcgacg agctggcgtt gctggaggac gtcggtggcg
     2761 cgggcctggc cgaggcggaa gcgggcctcg atgagttcga ggatgatgcg ggcggtttcg
     2821 gtctgctcgt caagcagggc gaggcgcgcc tgctgggcac gcaggtcata ccaggcgagg
     2881 gcgacctcgg ccgggctgac cgccagccgc cgccggcgct cgtagagctg gcggtacatg
     2941 a
//
LOCUS       DAOPCO010000402         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26799_length_2926_cov_4.673285, whole genome shotgun sequence.
ACCESSION   DAOPCO010000402 DAOPCO010000000
VERSION     DAOPCO010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26799_length_2926_cov_4.673285"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="PLS90_16920"
     CDS             complement(<1..379)
                     /locus_tag="PLS90_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04129.1"
                     /translation="MPRAQPITPHEPPSPRPARRGLRAGAAQTEITPRRPMFLFGYPH
                     VERTSTGVNDPLLASALYLETGGGRLLLLSVDLCCVTREITERVRAGLRDAAGLAPGE
                     IVLGATHTHSGPVTASLQVCRDDA"
     gene            526..1305
                     /locus_tag="PLS90_16925"
     CDS             526..1305
                     /locus_tag="PLS90_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016484137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK04130.1"
                     /translation="MLVSRQHIDQYNRDGFFLLDHVLSDDQLEGLRGECSRFIHAEDR
                     RMDELGTDTRDLNFRGKRYFLGMPHRESTVLTDFIFSPTMEELTRAVLGPDVWLYFTQ
                     YVVKGPEVGLKFAWHQDSAYIGHPHAPYLSCWIPLDDVTVENGTVYVLPYDRGGGRRL
                     VEHTKDPELNDLVGYHGDDPGIPAILPAGGMAVFSSLTLHRSGPNTTPRMRRIYLIQY
                     SPEPLMNKSGTGPFRDAIPFLKDGERIVNESLIPREVWVAT"
     gene            complement(1316..>2926)
                     /locus_tag="PLS90_16930"
     CDS             complement(1316..>2926)
                     /locus_tag="PLS90_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPK04131.1"
                     /translation="EDLDGAAPPEILIQGDGGLEIGRWNGNAIEIVDTLAGTEPVFAP
                     PAPLRDPTRFSPRAQFSNPHLWRPSPEASTFFLKDAGGVFVAELNADGQLRKVADAPA
                     DHPATGFVPVAERMKTTLAAAGNLHQILQEGQVLHEVEITSERQYLAPPALVGALGGA
                     VRILAKTAEANIESITPAGESDGILLKQHPATAGLHVEPTCLGSILDVDGDGENEFLS
                     STAAGGPAIVAVDAAGQEKLRIAPAGPLSELVLVATGKRNDQPGNWIVARWQTEPKTM
                     GVAWFVSGLGIDGSPLWQRTGYGMYQGEPTQFLQRAPTAVADYNADGGDDLFVSSENF
                     FGIMAGTDGTDLLGPKNILDKRPDDIWPAYSTPILVDLDGAAPPEMIHARSFGSTYVA
                     TWESEPLLSVKLSRDTTARRFPAAADLDGNGALEVITSQLNGLLIAYNVLPPSGTPGE
                     RWRHPLPGTVSDLISADLDDDGREELLVGCEDGRLYALREDAGQCAVLWSVDLGAAVG
                     SPILADMGEAGGPAILVPTEDGWLYCLR"
BASE COUNT          467 a          995 c          976 g          488 t
ORIGIN      
        1 ccgcatcgtc gcggcagacc tgaagactgg ccgtcaccgg gcccgagtga gtgtgggtgg
       61 cccccagcac gatctctccg ggcgccagcc ccgcggcgtc gcgcaggccg gcgcggaccc
      121 gctcggtgat ctcccgcgtg acgcagcaca ggtccaccga gaggagcagc aggcgcccgc
      181 cgccggtctc gaggtagagg gccgaggcga gcaacgggtc gttgaccccc gtgctggtcc
      241 gctccacatg ggggtagccg aagaggaaca tcggccggcg cggcgtgatc tcggtctgag
      301 ccgcgccggc ccgcaggccc cgtcgcgcgg gacgcggcga gggcggttca tggggcgtga
      361 tgggctgggc gcgcggcata gttgggaggg atcccaggcg tcgtgtgatc aggcaaaagg
      421 gccgaaaatc ccaattgaca ctcgtgtatc ataggcgtac catccgtgta aagcacaaac
      481 ggattggctt gctatctgtc tacagtcaac ttcggaggag tgacaatgct ggtctcccgt
      541 cagcacatcg atcagtataa ccgagacggc ttctttctgc tggaccacgt cctgtcggac
      601 gaccagttgg aggggctgcg cggggagtgt tcccgcttca tccacgccga ggaccgccgc
      661 atggacgaac tcggcaccga cacgcgcgac ctcaacttcc gcggcaagcg ctattttctc
      721 ggcatgcccc atcgcgagag cacggtgctg accgacttca tcttctcccc gacgatggag
      781 gaactgaccc gcgccgtcct cggccccgac gtctggctct atttcacgca gtacgtcgtc
      841 aagggaccgg aagtggggct caaattcgcg tggcaccagg attcggctta catcggtcat
      901 ccgcacgcgc cgtatctctc ctgctggatc ccgctcgacg acgtcacggt ggaaaacggg
      961 acggtctacg tcctgccgta tgatcgcggc ggcggacgcc gcctggtcga gcacaccaag
     1021 gaccccgagc tcaacgatct ggtcggctat cacggcgatg atccgggcat ccccgcgatc
     1081 cttcccgccg gcgggatggc ggttttctcc agtctgacgc tccaccgcag cggccccaac
     1141 acgacccccc ggatgcggcg catctacctg atccagtact cgcccgagcc gctgatgaac
     1201 aagtcgggga ccgggccgtt ccgcgacgcg atcccgtttc tgaaggacgg cgaacggatc
     1261 gtcaacgagt cgttgatccc gcgcgaggtc tgggtcgcca cctgaccggc gcggctcagc
     1321 gcaggcagta aagccagcca tcctcggtgg ggacgaggat cgcgggacct ccggcttctc
     1381 ccatgtcggc caggatgggc gagccgaccg cggcgcccag gtcgaccgac cagagtacgg
     1441 cgcactggcc cgcgtcttca cgcaaggcgt agagccgtcc atcctcgcat ccgacgagca
     1501 gttcctcgcg accgtcatcg tccaagtccg cacttatcag gtcgctcacc gtccccggca
     1561 gcgggtggcg ccagcgttcg cccggcgtgc ccgacggggg caggacgttg taggcgatca
     1621 gcagcccgtt aagctggctc gtgatcactt cgagcgcgcc gttgccgtcc agatcggccg
     1681 ccgcggggaa gcgccgggcc gtcgtgtcgc ggctgagctt gaccgacagc agcggctcgg
     1741 attcccaggt ggcgacatac gtcgaaccga acgagcgcgc gtggatcatt tcgggcggcg
     1801 ccgcgccatc caggtcgacc aggatcggcg tcgaatacgc cggccagatg tcatcgggtc
     1861 gtttgtcgag gatgttcttc ggcccgagca gatccgtgcc gtcggttccc gccatgatcc
     1921 cgaagaaatt ctccgaggaa acgaagaggt cgtcgccgcc gtcggcgttg taatccgcca
     1981 ccgccgtggg ggcccgttgc aggaactgcg tcggctcccc ctggtacatg ccataaccgg
     2041 tgcgctgcca cagggggctg ccgtcgatac cgaggcccga gacgaaccag gccacgccca
     2101 tggtcttcgg ctcggtctgc cagcgcgcca cgatccagtt gccgggctgg tcgttgcgct
     2161 tgccggtcgc cacgaggacg agttcgctca gcgggcccgc gggggcgatg cgcaactttt
     2221 cctggcccgc ggcatcgacg gcgacgatcg ccgggccgcc cgccgccgtg ctgctcagga
     2281 actcgttctc gccgtcgccg tcgacgtcca ggatcgagcc gaggcaggtc ggttccacgt
     2341 gcaggccggc ggttgccgga tgctgcttga gcaggatccc atcgctttcg cccgccggcg
     2401 tgatcgactc gatgttcgct tcggccgtct tggcgaggat gcggaccgct ccgccgagcg
     2461 cgccgacgag ggccggcggc gccaggtact gccgttcact ggtgatttcc acctcgtgca
     2521 ggacctgtcc ctcctgcagg atctggtgca aattgcccgc ggcggccagc gtggttttca
     2581 tcctttcggc cacggggacg aaccccgtcg ccggatgatc cgccggcgca tcggcgacct
     2641 tgcggagctg cccatccgcg ttcaattcgg caacgaatac gccgccagca tccttgagga
     2701 agaaggtgct cgcctcgggc gaggggcgcc agaggtgggg attgctgaac tgggcgcggg
     2761 ggctgaaccg cgtcggatcg cgcaacggcg cggggggcgc aaacacgggc tcggtccccg
     2821 ccagcgtatc gacgatttca atcgcgttgc cattccaccg cccgatctcg aggccgccgt
     2881 caccctgaat caggatctcg gggggcgccg cgccgtccag gtcctc
//
LOCUS       DAOPCO010000403         2918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26905_length_2918_cov_3.697869, whole genome shotgun sequence.
ACCESSION   DAOPCO010000403 DAOPCO010000000
VERSION     DAOPCO010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26905_length_2918_cov_3.697869"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /gene="sucD"
                     /locus_tag="PLS90_16935"
     CDS             <1..790
                     /gene="sucD"
                     /locus_tag="PLS90_16935"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPK04132.1"
                     /translation="GTQVVAGVTPGRGGQTCLDRPVYDTMQEAVRETQPNACCVFVPA
                     RFAADAALEAIDAEIPLVIVITEGIPTLQMMKVYWEAQRRGVRVIGPNCPGVITPGEA
                     KIGIMPGHIHMPGKVGVVSRSGTLTYEFVNELTRENFGQTTCIGIGGDPIVGTDFVDS
                     LRLFNEDPATEAVVMIGEIGGAAEERAAEYVKREMVKPVVGFIAGRTAPPGKRMGHAG
                     AIIAGGSGTAAEKISALEAAGIAVCERPGQVGEKLRQAGVRPLS"
     gene            1216..1386
                     /locus_tag="PLS90_16940"
     CDS             1216..1386
                     /locus_tag="PLS90_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04133.1"
                     /translation="MRIPVTGDMLYDFVLVVILIVVGMGLALKADKWWGYILVVVAGF
                     WALQLVRPIMGM"
     gene            1460..2068
                     /gene="pgsA"
                     /locus_tag="PLS90_16945"
     CDS             1460..2068
                     /gene="pgsA"
                     /locus_tag="PLS90_16945"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPK04134.1"
                     /translation="MNLSNKLTVLRILIVPVFLLALTPGRFLLPAAADLWLHPLALVL
                     FIIAAITDYYDGMLARRHGWVTNFGKLMDPLADKVLVMAAFVGMVELRLFAAWMVAFI
                     LAREFLITGLRLLATAEGRVLAADRWGKNKTITQMTAIIAGLVFLIWRDIVEQFALPE
                     AARASVGYLHGGLQALMLATVVLTIASGARYFIANRDVIAER"
     gene            complement(2075..>2918)
                     /locus_tag="PLS90_16950"
     CDS             complement(2075..>2918)
                     /locus_tag="PLS90_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04135.1"
                     /translation="TQAWVVFSLWWVLIVAALTLKLADPSRRRTLALHAAWFISGLFL
                     YGITNLYAVQAALLHAAARRGAVARRAAGSLLFALALLALGLAASCATGAYMDVRHEF
                     WIVTPSARGGSLSWILLRSLANFLLCSVSLPIQGRSLDPNPGIPGEWILSFPRLAYSP
                     LGWVLAGLWTAWLLAAARAALRAGDPPVRRLALGQAACVGFFIVFHCFYYVPWEGLWL
                     YTPAVVPALVAFQVPLLARVAAAPPRRRLALRLALLLFIALLALRHARFLLALPEIFA
                     PVWR"
BASE COUNT          519 a          755 c         1228 g          416 t
ORIGIN      
        1 cggcacgcag gtggtggcgg gggtgacgcc gggccgcggg ggccagacgt gtctggaccg
       61 gccggtgtat gacacgatgc aggaggcggt gcgcgagacg cagccgaacg cctgctgcgt
      121 gttcgtgccg gcgcggttcg cggccgacgc ggcgctggag gcgatcgacg ccgagattcc
      181 gctggtgatc gtgatcaccg agggcatccc gacgctgcag atgatgaagg tctactggga
      241 agcgcagcgc cggggggtgc gcgtgatcgg gccgaactgc ccgggggtga tcacgccggg
      301 ggaggcgaag atcgggatca tgccgggtca tatccacatg ccgggcaagg tgggggtcgt
      361 gtcgcggtcg gggacgttga cgtatgagtt tgtgaacgag ctgacgcggg agaatttcgg
      421 gcagacgacg tgcatcggga tcggcggcga cccgatcgtg gggaccgatt tcgtggattc
      481 gctgcggctg ttcaacgagg atccggcgac cgaggcggtc gtgatgatcg gcgagatcgg
      541 cggcgcggct gaagagcgcg cggcggagta tgtcaagcgc gagatggtga agccggtggt
      601 ggggttcatc gccggacgga cggcgccgcc gggcaagcgg atggggcatg ccggggcgat
      661 catcgccggg gggagcggga cggcggcgga gaagatatcg gcgctcgaag cggcggggat
      721 tgccgtctgc gagcggccgg ggcaggtcgg cgagaagctg cgccaggcgg gtgtgcggcc
      781 gctgagctga gcgcgcggcg ggggcggaga aaagcgcttt gcgccggcgc tgcggtggac
      841 tatcctttca gctggaagct gtgacaatcg acgttgcggg aatggacctg gcccgagcct
      901 tcggggactg ggggatgggg gtttggctgt cccggggttc cgccatgaag caagggatca
      961 ggaacgacac ggatgagcac gaaagggcga tccgtggggc gcagggcgga ggcgactcgg
     1021 cggagggttt tcgagagccg aggacgagag ccgagagccg aagtttgggg gggcgttgag
     1081 ggcgattacg agttgctcat gaaccggggg tgcacaagga ggggacaaaa ctgggcaggc
     1141 cttcgggagc gagagcgagg gacgaggacg agggacggag aattgttcga aagacgcggt
     1201 gagagggggt gggcgatgag aattccggtg acaggggaca tgctgtatga tttcgtgttg
     1261 gtcgtgatcc tgatcgttgt cgggatgggg ctggcactga aggccgacaa atggtgggga
     1321 tacattctgg tggtggtggc gggattctgg gcgctgcaac tggtgcggcc gatcatgggg
     1381 atgtaacgcg cggagtcggg cgtccggccc gccgaggtcg cccggccgaa cgcggcaatc
     1441 gggcagcagc cggggatcga tgaacctatc caacaagctg accgtccttc gcatcctgat
     1501 cgttccggtg tttctgctgg cgttgacgcc ggggcggttt ctgctgccgg cggcggccga
     1561 cctgtggctg catccgctgg cactcgtgct gttcatcatc gcggcgatca ccgattacta
     1621 cgacgggatg ctggcgcggc gccatggctg ggtcacgaat tttggcaagc tgatggatcc
     1681 gctggccgac aaggtgctgg tgatggcggc gttcgtgggg atggtggagc tgcggctgtt
     1741 tgcggcgtgg atggtggcgt tcatcctggc gcgcgagttt ctgatcacgg ggctgcggct
     1801 gctggcgacg gcggaggggc gcgtgctggc ggcggaccgc tgggggaaga acaagacgat
     1861 cacgcagatg acggcgatca tcgcggggct ggtgtttctg atctggcgcg acatcgtcga
     1921 gcagtttgcg ctgcccgaag cggcgcgggc gagcgtcggc tatctgcacg ggggactgca
     1981 ggcgctgatg ctggcgacgg tggtgctgac gatcgccagc ggcgcgcgct acttcatcgc
     2041 caatcgcgat gtgatcgcag agcgctgaac cgggttagcg ccagacgggg gcgaagatct
     2101 cggggagcgc gagcagaaag cgggcgtggc gcagcgcgag cagcgcgatg aagagaagca
     2161 gggcgagacg cagggccagg cggcggcgcg gcggggcggc ggcgacgcgg gcgagcaggg
     2221 gaacctggaa ggcgacaagg gccggcacga cggcgggggt atagagccag agcccttccc
     2281 acggaacata ataaaaacaa tgaaacacga tgaagaagcc gacacaggcg gcctggccga
     2341 gcgccagccg gcggacgggc gggtcgccgg cgcgcaacgc cgcgcgcgcg gcggccaaca
     2401 gccatgccgt ccacaggccg gcgaggaccc agcccagcgg cgagtaggcg agcctgggga
     2461 acgagaggat ccattcgccc gggatgccgg ggttgggatc gagtgaacgc ccctggatgg
     2521 ggaggctgac tgaacagagg aggaagttgg cgagcgaacg caggaggatc caggagaggc
     2581 tgcccccgcg tgcggagggg gtgacgatcc agaattcgtg gcggacatcc atataggcgc
     2641 cggtggcgca actggcggcg aggcccaggg cgagcagggc gagggcaaag aggagcgagc
     2701 cggccgcgcg gcgggcaacc gcgccccggc gcgcggcggc gtggagcaag gccgcctgaa
     2761 cggcgtagag gttcgtgatg ccgtagagga acaggccgga gatgaaccag gcggcgtgga
     2821 gggcgagcgt ccggcggcgc gaggggtcgg caagcttcag ggtcagcgcg gccacgatca
     2881 ggacccacca gagggaaaag acgacccagg cttgcgtt
//
LOCUS       DAOPCO010000404         2914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_26938_length_2914_cov_4.920252, whole genome shotgun sequence.
ACCESSION   DAOPCO010000404 DAOPCO010000000
VERSION     DAOPCO010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26938_length_2914_cov_4.920252"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /gene="acs"
                     /locus_tag="PLS90_16955"
     CDS             complement(<1..1587)
                     /gene="acs"
                     /locus_tag="PLS90_16955"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPK04136.1"
                     /translation="MAVTQEAIADLLQEDRHFEPSDAFRAQANINDPTIYDRAAADPE
                     TFWADFAKELHWHEPWHTVLDWNPPHAKWFVGGRTNVCYNCVDRHIKGPRRNKAALIW
                     EGENYDQRTLTYWDLYREVNKAGNMLRSFGVRKGDRVAIYLPMIPEAVIAMLACARIG
                     AVHSVVFGGFSAESLADRINDSKCKVLITADGGFRRGGLLPLKHDADYALQQCPSIEK
                     VVIVKRGDFPLRVKEGRDYWWHRLMDDQPQYCEPEWVDSEDLLYILYTSGTTGKPKGI
                     MHTTGGYMVHCYATSKWVFDLKEDDVFWCTADVGWVTGHSYLTYGPLANGATCVMYEG
                     APNWPDNDRFWRIVEKYGVTIFYTAPTAIRAFMKWGRKWPQSCDLSSIRLLGTVGEPI
                     NPEAWVWYYRFIGGERCPIIDTWWQTETGGIMITSLPGITTMKPGSATRPFPGISAEI
                     LNDAGEVVPTGGGYLALTRPWPGMLRGIYGDMERYENTYWSKWGKSIYFPGDGAKRDE
                     DGNFTVIGRVDDVINIAGHRI"
     gene            complement(1891..>2914)
                     /locus_tag="PLS90_16960"
     CDS             complement(1891..>2914)
                     /locus_tag="PLS90_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPK04137.1"
                     /translation="ELLDLRTLSLLGLLTLALIASIYLARPILMPIMLAFLLHLILRP
                     VHNTLLRFHLHRILSACLILGLLGGAVAWGIANLTEPAREWLDEIPATVRQTQWKLRE
                     LLEPVGRVTQLTEQVTELAKIEPADPAPRVQVAQGSATNLIWNTAQQVIASFSITFIM
                     LFFMLAYGERITRNMRARSKAVVLATEIGGHVSQYLLTVTLINVGLGVCIGAALYLLG
                     MPNAILWGVMGFVLNFIPYLGALVGVAIVFFAALIAFDGNTQIALIPLTYFALTAAEG
                     NFITPMILGRRFTLNPIFIFLALVFWGWMWGVMGALMAVPLLMAFKIVCDHIRSLQWL
                     SQVMSD"
BASE COUNT          533 a          738 c         1133 g          510 t
ORIGIN      
        1 gatgcgatgg ccggcgatgt tgatgacgtc gtcgacgcgg ccgatgacgg tgaagttgcc
       61 gtcctcatcg cgtttggcgc cgtcgccggg gaagtaaatc gacttgcccc acttggacca
      121 gtaggtgttt tcgtagcgtt ccatgtcgcc gtagatcccg cgcagcatgc cgggccaggg
      181 cctggtcagg gcgaggtagc cgccgccggt ggggacgacc tcgccggcgt cgttgaggat
      241 ttcggcggag atgccgggga aggggcgggt ggcggagccg ggcttcatgg tggtgatgcc
      301 ggggagcgag gtgatcataa tgccgccggt ttcggtctgc caccaggtgt cgatgatggg
      361 acagcgttcg ccgccgatga agcggtagta ccagacccag gcctcggggt tgatcggttc
      421 gccgacggtc ccgagcaggc ggatggagga gaggtcgcag ctctggggcc acttgcgccc
      481 ccatttcatg aaggcgcgaa tggcggtggg ggcggtgtag aagatggtga cgccgtattt
      541 ttcgacgata cgccagaagc ggtcgttgtc gggccagttg ggggcgcctt catacatgac
      601 gcaggtggcg ccgttggcga gcgggccgta ggtcaggtag ctgtggccgg tgacccagcc
      661 gacgtcggcg gtgcaccaga agacgtcgtc ttccttgagg tcaaaaaccc acttcgacgt
      721 ggcataacag tggaccatgt agccgccggt ggtgtgcatg atccccttgg gtttgccggt
      781 ggtgcccgag gtatagagga tgtagagcag atcctcgctg tcgacccatt ccggttcgca
      841 gtactgcggc tggtcgtcca tcaggcgatg ccaccagtag tcgcggccct ccttgacccg
      901 gagggggaaa tcgccgcgct tgacgatgac gaccttctcg atggaggggc actgctggag
      961 ggcatagtcg gcgtcgtgct tgagcgggag gaggccgccg cgacggaagc cgccgtcggc
     1021 ggtgatgagg accttgcact tggagtcgtt gatgcggtcg gcgagggact cggcggagaa
     1081 gccaccgaag acgacggagt ggacggcgcc gatgcgggcg caggcgagca tcgcaatgac
     1141 ggcctcgggg atcatcggca ggtagatggc gacgcgatcg cccttgcgca cgccgaagga
     1201 gcgcagcatg ttgccggcct tgttgacctc gcggtagaga tcccagtagg tcagggtgcg
     1261 ctgatcgtag ttttcaccct cccagatgag ggcggccttg ttgcggcggg ggcccttgat
     1321 gtgacggtcg acgcagttgt agcagacgtt ggtgcgtccg ccgacgaacc acttggcgtg
     1381 gggcgggttc cagtccagga cggtgtgcca cggctcgtgc cagtggagtt ccttggcgaa
     1441 gtcggcccag aaggtttcgg gatcggcggc ggcgcgatca tagatggtgg gatcgttgat
     1501 attggcctgg gcgcggaatg cgtcgctcgg ttcgaagtgg cgatcctcct ggagcagatc
     1561 ggcaatcgcc tcttgcgtga cagccatggt cgggacctcc tgcggccggt gggcggccgg
     1621 atcagtgaaa tggggcggga aaaaacgaca cgagagtcat cttaacaggc tgacgaatca
     1681 tactcccctt gtggactccc ccaagtcgtc tcgccggtgg aaggttttca gtttaaaccg
     1741 gttgagcggt tgcgtcaata aggaggtccg aaccggagtt gttgcaatga tccgtgaagg
     1801 gacggggggc ctcgagcgtc ccaggctggt ggttgatctg gcgaatgccc gactttgaga
     1861 gccgagaacg tggacgagag acgaattgag ctaatcggac atgacctggc tgagccattg
     1921 gagcgagcga atgtggtcgc agacgatctt gaacgccatc aggaggggga cggccatcag
     1981 ggcgcccatg acgccccaca tccagcccca gaagacgagg gcgaggaaga tgaagatcgg
     2041 attcagggtg aagcggcggc cgaggatcat cggggtgatg aagttgccct cggcggcggt
     2101 cagggcgaaa taggtgagcg gaatgagcgc gatctgggtg ttgccgtcga aggcgataag
     2161 ggcggcgaag aagacgatcg cgacgccgac gagggcgccc aggtagggga tgaaattgag
     2221 aacgaagccc atgacgcccc agaggatggc gttgggcatg ccgagcaggt agagcgcggc
     2281 gccgatgcag acgcccaggc cgacgttgat cagggtgacg gtgagcaggt actgggagac
     2341 gtggccgccg atttcggtgg cgaggacgac ggccttgctg cgggcgcgca tgttgcgggt
     2401 gatgcgctcg ccgtaggcca gcatgaagaa gagcatgatg aaggtgatgg agaaactggc
     2461 gatgacctgc tgggcggtgt tccagatgag gttggtggcg gagccctggg cgacctgaac
     2521 gcggggggcg gggtcggccg gttcgatctt ggccagttcg gtgacctgct cggtgagctg
     2581 ggtgacgcgg ccgaccggct cgagcaattc gcgcagtttc cattgggtct ggcggaccgt
     2641 ggcggggatt tcgtcgagcc actcgcgggc gggctcggtc aggttggcga tgccccaggc
     2701 gacggccccg cccaggaggc cgaggatcag gcaggcggag aggatgcggt gcaggtggaa
     2761 acggaggagg gtgttgtgga cggggcgcag gatgaggtga agcagaaagg cgagcatgat
     2821 cggcatcagg atggggcggg cgagataaat ggaggcgatg agcgccaggg tgagcaggcc
     2881 gaggagcgag agggtgcgca ggtcgaggag ctcg
//
LOCUS       DAOPCO010000405         2903 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_27075_length_2903_cov_5.729635, whole genome shotgun sequence.
ACCESSION   DAOPCO010000405 DAOPCO010000000
VERSION     DAOPCO010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27075_length_2903_cov_5.729635"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PLS90_16965"
     CDS             <1..1685
                     /locus_tag="PLS90_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04138.1"
                     /translation="AAMARAEGLAVGVYLHCGSFSDNFLDSRPDAADWLAWPAPGKPP
                     ERIAFAAADTPAGGNKHRTRYPVYRNHPEYLALLKELVAVAINEYKANLIHFDNWCNG
                     AGWSPRAIQDFGAFLAARHGEDGWRERLGADNLDELAELAQRDGTPAQAEWRYFEAWS
                     LGDALRQLARYIRELNPDVAVEINSSSFTINYPVPVDVSQLLPYVDAFWHEQTEQGWQ
                     ADSKRFVSSFRSLKLAQRFDASLFSYTPTRLSFCEALAGNVDTVGCPYWFLYGRLNWP
                     PWEPWCYEPSTDLLAPETRFFFEHRELFVERKPYVEVATLRGRATNLAGPRQAAENAY
                     LMEQVLLIGHLPFTILFDQDLIDLARYRAVVLPDVRTLEPRQIDLLLRYVEGGGHLLV
                     TDQTATLDIHGRPDDSGFRRLFGVAPNRAEPGLVVQQGAGRIAYLRTERDAEIEKGSL
                     PRNWRALKEGIEQLMGPSAVQTDAPIHLAMEFYRDERAVRVHLVDYSENPEHPGFSIT
                     LSHRLGRPRSARLLAPRREPLALTVTRQADTSTISIPGLDLYGVVDCELDND"
     gene            1712..2863
                     /locus_tag="PLS90_16970"
     CDS             1712..2863
                     /locus_tag="PLS90_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04139.1"
                     /translation="MQPTTTTLHVRAHILRNTSFTNWDAEVRGRWDWHQLMADENYRK
                     GWISFDGVLWHPELKQLFCGLMELEADIFYRFDPASGAFQSLGFREHGSPYDAKFHRS
                     MLLDDDGALFCATSMLHDPDRQAEAPGGRIIHYDPRAHAYRQVAIPIPRQYIQSIALD
                     RRRRLIYGFTYPAEYFFQHDLKTGETRQLAFLGNPNGMCQPHVAVVDRAGWLWGQWGE
                     NRAFDDVPCLNLHLFKYHPDEGRIHWLRHTLARPRDGDVGAIDGMCLAPDGNIYIGTK
                     GASLFRLRPEAEQLEYLGRPGPRARMSGMVAGPDGRIYLACGEREECGLYSLDPAHDT
                     LTHHGHIRDEALGASAERIHDLTVTEDLVLYAGENDNHDRSSYLWELRP"
BASE COUNT          481 a         1072 c          868 g          482 t
ORIGIN      
        1 tcgccgcgat ggcccgcgcc gagggccttg ccgtcggcgt ctacctgcac tgcgggtcgt
       61 tctccgacaa cttccttgat tcgcgccccg acgccgccga ctggctcgcc tggcccgcgc
      121 cgggcaagcc gcccgaacgg atcgcgttcg cggccgccga tacccccgcc gggggcaaca
      181 agcaccgcac gcgttatccg gtctaccgca accacccgga gtaccttgcg ctgctcaagg
      241 aactggtcgc ggtcgcgatc aacgaataca aggccaacct gatccatttc gacaactggt
      301 gcaacggggc ggggtggagc ccgcgcgcga tccaggattt cggcgccttt ctggccgcgc
      361 gccacggcga ggacggatgg cgcgaacggc tcggcgccga caatctggac gaactcgctg
      421 agctagccca gcgcgacggc acgcccgccc aggccgaatg gcggtatttc gaggcctggt
      481 cgctcggcga cgcgctgcgc cagctcgccc gctacatccg cgaactcaac cccgacgtcg
      541 ccgtcgagat caactcgagc agcttcacga tcaactaccc ggttcccgtc gatgtctccc
      601 agctgctgcc ctacgtcgac gccttctggc acgagcagac cgaacagggc tggcaggccg
      661 attccaagcg cttcgtctcg tccttccgct cgctcaagct ggcccagcgc ttcgacgcct
      721 cgctgttctc ctacacgccg acgcgcctat ccttttgcga ggcgctcgcg ggcaacgtgg
      781 atacggtcgg ctgcccctac tggtttctct atggccggct caactggccg ccgtgggaac
      841 cgtggtgcta cgagccgagc actgacctgc tcgccccgga aacccggttc ttcttcgagc
      901 accgcgagct gttcgtcgaa cgcaagccgt atgttgaggt cgccacgctg cgcggccgcg
      961 cgaccaacct ggccgggccg cggcaggcgg ccgaaaacgc ctatctgatg gaacaggtcc
     1021 tcctgatcgg ccacctcccg ttcaccatcc tgttcgatca ggacctgatc gacctggcgc
     1081 gctaccgcgc cgtcgtgctg cccgacgtcc gcacgctcga gccgcggcag atcgatctcc
     1141 tcctgcgcta cgtcgagggg ggcggccatc ttctcgtcac cgaccagacg gccacgctcg
     1201 acatccatgg ccggcccgac gacagcggct ttcgccggct gttcggcgtg gcgcccaacc
     1261 gcgccgaacc cggcctggtc gttcagcagg gcgccggccg gatcgcctat ctgcgcaccg
     1321 aacgcgacgc cgaaatcgag aaggggtcgc tcccgcgcaa ctggcgcgcg ctgaaggagg
     1381 gcatcgaaca gctcatgggc cccagcgcgg tccagaccga cgcgccgatc cacctcgcca
     1441 tggagttcta ccgcgacgag cgcgccgttc gcgtgcatct cgtcgattat tccgagaacc
     1501 ccgagcaccc cgggttctcg attacgcttt cgcaccggct gggccggccc cggtccgcgc
     1561 gtctgctcgc accccgccgc gaacccctcg cgctcacggt gacgcggcag gccgacacct
     1621 ccacgatcag catcccgggg cttgatctct acggcgtcgt cgattgcgaa ctggacaacg
     1681 attgaaacgg cggaaagcga caaccgacat catgcaaccc accaccacga ccctgcacgt
     1741 gcgcgcgcac atcctgcgca acacgagctt caccaactgg gacgccgaag tccgcggccg
     1801 ctgggactgg caccagttaa tggccgatga gaattaccgc aagggctgga tctcgttcga
     1861 cggcgtgctc tggcaccccg agctcaaaca gctcttctgc ggcctgatgg agctcgaagc
     1921 ggacatcttc tatcgcttcg acccagccag cggcgctttc cagtccctcg gctttcgcga
     1981 acacggctcg ccctacgacg ccaaattcca ccgcagcatg ctgctggacg acgacggcgc
     2041 cctcttctgc gcaacctcca tgctgcacga tcccgaccgc caggccgagg cccccggcgg
     2101 ccgcatcatc cattacgatc cgcgcgccca cgcctaccgt caggtcgcca tcccgatccc
     2161 gcgccaatat attcagtcga tcgcgctgga tcgccggcgg cgtctgatct acggcttcac
     2221 gtatccggcc gagtattttt tccagcacga tctgaaaacc ggcgagaccc ggcagctcgc
     2281 ctttcttggg aatcccaacg gtatgtgcca gccgcatgtt gccgtcgtcg accgcgcggg
     2341 ctggctctgg gggcagtggg gcgagaaccg cgcattcgac gacgtcccct gcctgaatct
     2401 ccatctgttc aagtatcacc ccgacgaggg gcggatccat tggctgcgcc atacgctcgc
     2461 ccgcccgcgc gacggcgacg tgggcgccat cgacgggatg tgcctcgcgc ccgacggcaa
     2521 catttacatc ggcaccaagg gcgccagcct gttccggctg cgccccgagg ccgagcagtt
     2581 ggagtatctc ggccgccccg gtccgcgcgc ccggatgtcc ggcatggtcg ccggccccga
     2641 cgggcggatc tatctcgcct gcggtgaacg cgaggagtgc gggctctaca gtctcgatcc
     2701 ggcccatgat acgctcacgc atcatgggca catccgcgat gaagcgctcg gggcgtcggc
     2761 cgaacgaatc cacgacctga ccgtcaccga ggatctggtc ctttacgccg gcgaaaatga
     2821 caaccatgat cgctccagct acctctggga gttgcgcccc tgaggagcgg aggcgatggt
     2881 gtcgctgcgt tcaaccaccg gct
//
LOCUS       DAOPCO010000406         2878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_27361_length_2878_cov_4.286575, whole genome shotgun sequence.
ACCESSION   DAOPCO010000406 DAOPCO010000000
VERSION     DAOPCO010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27361_length_2878_cov_4.286575"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            533..1648
                     /gene="rlmN"
                     /locus_tag="PLS90_16975"
     CDS             533..1648
                     /gene="rlmN"
                     /locus_tag="PLS90_16975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPK04140.1"
                     /translation="MSPIAAEMQEPPAELRGLTPAELTALLAEGDFEPYRARQLYHWL
                     YVKGAESLEEMTNLPRALREWLGERTRVGAIELARIRGAEGATRKALFRLGDGKFIES
                     VLMRDEPVAVDDTEAAAAPARISFCLSSQVGCALGCTFCRTGFGGFQRNLTPGEIVGQ
                     ALTMRRRLLAAGETLQHIVFMGMGEPMHNLDNVLTALRLLTDPEGFKLSRRRITVSTV
                     GWVPGIERLGASGVGVNLAVSLNATTDETRSTIMPVNRRYPIGALLEALRRYPLEPRR
                     RITIEYVLLRGVNDTPADARRLVGLLRGLRCKVNLIMFNACPELAYEPVEAEGLDRFA
                     AVLSEANMTVTVRWSKGRELEAACGQLAAHVLEAARG"
     gene            1675..2457
                     /gene="pgeF"
                     /locus_tag="PLS90_16980"
     CDS             1675..2457
                     /gene="pgeF"
                     /locus_tag="PLS90_16980"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPK04141.1"
                     /translation="MTCRRELTVSCADWAEVPGLVHGTTTRAALPEPGKLDFFDAVER
                     ARGVGALPEWPAVGADQVHGAHLEVIAELDGLRARGVRRNEGADAYEFPETDALIALA
                     PGVLLVIQTADCLPVLLVDAERRRVGLAHCGWRGLLAGLAEKTARAMIEAGSRPAALR
                     AWLGPCAGAARYEVGCELVDRFRRAFAEIDPAVISPEGTHLDLAAIARERLIGAGVEA
                     GNILASGECTLTDRARYHSYRAEGERAGRMLSFIGFEASAAR"
     gene            complement(2464..2646)
                     /locus_tag="PLS90_16985"
     CDS             complement(2464..2646)
                     /locus_tag="PLS90_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04142.1"
                     /translation="MARPHDIAMCPRAITRRLKEVFELNRLCRILGKARPLGPAEPPD
                     PNAPPDRKPPRDRASE"
     gene            complement(2698..>2878)
                     /locus_tag="PLS90_16990"
     CDS             complement(2698..>2878)
                     /locus_tag="PLS90_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04143.1"
                     /translation="AWGWTDEGKMARVRIAAPGAGARNPAFDVTPAALITGIITEHGI
                     IPPTRDAIARTIAKP"
BASE COUNT          414 a          785 c         1213 g          466 t
ORIGIN      
        1 agcacgaaat tcgaaatccg aaattcgtaa taaagtggat ccaaagccgt gtcgacgcgt
       61 cgtggcgaag gtctggccgc gcgttccaag aagacggcgt gagctcgctc cgatcggggg
      121 tgcggcggat ggggctgaca tgggggtttc ctgggtggga tggaaagcgt tgaaacggtt
      181 taaaaaaact atcgcgttcg ggaaccggcc gaggcccggt gctaatatcc gtgcctgtgg
      241 gggttccaat ggaaagcccg ctagcgcggg ctggcgggtg ggcttgcgcc gccgaatccg
      301 acgggttatt tgccgcagga tctctggcat ccctggcggg atgcgggggg atcgagaggc
      361 gaggacgagg gacgagagcc gaatttcgga gtgagaaaaa gcgggggaat gaccggcgag
      421 ggcgccggtc ccacaccggt ggtggtgcgt cgctcggctg tttgcgggca tccgcggccg
      481 gcgatggctt cccatcggtg accggttagg ctaaagtggg gggatgacgt ccgtgtcgcc
      541 catcgccgcc gaaatgcagg agccgccggc cgaactgcgc gggctgacgc cggcggagct
      601 gacggcgctg ttggcggagg gggacttcga gccgtaccgc gcgcgccagc tctaccattg
      661 gctttatgtg aagggcgccg agtcgctgga ggagatgacg aacctgccgc gcgcgctgcg
      721 cgagtggctg ggcgaacgga cgcgcgtcgg ggcgatcgag ctggcgcgga tccgcggcgc
      781 ggaaggggcg acgcgcaagg cgttgttccg gctgggcgac ggcaagttta ttgaaagcgt
      841 gctgatgcgc gacgagccgg tggccgtgga tgatacggaa gcggcggccg cgccggcgcg
      901 gatcagcttc tgcctctcct cgcaggtcgg ctgcgcgctg ggctgcacgt tttgccggac
      961 ggggtttggg ggctttcagc gcaatctgac gccgggcgag atcgtggggc aggcgctgac
     1021 gatgcgccgt cggctgctgg cggcgggcga aacgctgcag cacatcgtgt tcatgggcat
     1081 gggcgagccg atgcacaacc tggacaacgt gctgacggcg ctgcggctgc tgaccgatcc
     1141 ggaggggttc aagctgtcgc ggcggcggat tacggtgtcg acggtcggct gggtgccggg
     1201 gatcgagcgg ctgggggcgt cgggcgtcgg cgtaaacctg gcggtgtcgc tcaacgcgac
     1261 gaccgacgag acgcggtcga cgatcatgcc ggtcaaccgg cgctacccga tcggcgcgct
     1321 gctggaggcg ctgaggcgat atccgctcga accgcgccgg cggatcacga tcgaatacgt
     1381 cctgctgcgc ggggtgaacg acacgccggc ggatgcacgg cggctggtgg ggctgctgcg
     1441 cgggctgcgc tgcaaggtca acctgatcat gttcaacgcc tgcccggagc tggcgtatga
     1501 gccggtcgag gcggaggggc tggatcggtt cgcggcggtg ttgtccgaag cgaacatgac
     1561 ggtgacggtg cgctggagca aggggcggga gctggaagcg gcctgcgggc agttggccgc
     1621 gcacgtgctg gaggcggcgc gggggtgagc ggccgtccat ggagttgatt gccgatgact
     1681 tgccgccgcg agctgaccgt ctcatgcgcc gactgggcgg aagtgccggg gctcgtccat
     1741 gggacgacga cgcgggcggc gctgcccgag ccgggcaagc tggacttctt tgacgcggtt
     1801 gagcgggcgc ggggggtggg ggccctgccc gagtggccgg ccgtcggggc ggaccaggtg
     1861 cacggcgcgc atctggaggt gatcgcggag ctggatgggt tgcgcgcgcg aggggttcgc
     1921 cggaacgagg gggccgatgc gtatgagttt ccggaaacgg acgcgctgat cgcgctcgcg
     1981 ccgggcgtgc tgctggtgat ccagacggcc gactgcctgc cggtgttgct ggtcgacgcg
     2041 gagcggcggc gggtggggct ggcgcattgc ggctggcgcg ggctgctggc ggggctggcg
     2101 gagaagacgg cgcgggcgat gatcgaagcg ggatcgcggc cggcggcgct gcgagcatgg
     2161 ctgggcccgt gcgcgggggc cgcgcgctat gaggtcgggt gtgaactggt ggaccggttc
     2221 cggcgagcgt ttgcggagat cgatccggcg gtgatttcgc cggaggggac gcatctggac
     2281 ctggcggcga tcgcgcggga gcggctgatt ggggccgggg tggaggcggg caacatcctg
     2341 gccagcggcg agtgcacgct gaccgatcgc gcgcggtatc attcctaccg agccgagggg
     2401 gagcgggccg gccggatgct gtcgttcatc ggatttgagg cgtcggcggc gcgctgacgg
     2461 ggctcattcg gatgcgcggt cgcgcggggg cttgcgatcg ggaggggcgt tcgggtcggg
     2521 cggttcggcg ggtccgagcg gacgggcctt gccgagaatg cggcagaggc gattcagttc
     2581 aaagacttcc ttgaggcggc gggtgatcgc acgggggcac atggcgatgt catgaggtct
     2641 cgccatggga tgtgcgcgct ccgcgagttt ttcaaaatca gccagcttcc aggggggtca
     2701 gggcttggcg atcgtccggg cgatggcgtc gcgggtcggc gggatgatgc cgtgctcggt
     2761 gatgatcccg gtgatgagcg cggcgggtgt gacgtcgaag gcggggttgc gcgcgccggc
     2821 gccgggggcg gcgatgcgga cgcgggccat tttgccctcg tcggtccagc cccaggca
//
LOCUS       DAOPCO010000407         2845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_27768_length_2845_cov_4.105376, whole genome shotgun sequence.
ACCESSION   DAOPCO010000407 DAOPCO010000000
VERSION     DAOPCO010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27768_length_2845_cov_4.105376"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PLS90_16995"
     CDS             complement(<1..1177)
                     /locus_tag="PLS90_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPK04144.1"
                     /translation="MKKQRTTLKDIAQEVGVSINAVSSVLNPRSPNNIGVSANTRSRI
                     MAAARRLNYQRNMAAAHLAGIRTKTVGILLTNLLDYRWLPVADAFEAEVNRLGYHCFI
                     GCTRYDGALKLDYVRSFLQHRVDGLLLNKIWNEPEVNQAMELISDTKLPIAFAHTRWQ
                     GFPAPVIQCDNYRGGQLLARHLLEVGHRTFIYLASAEDFSLETVQARISGARAEVTAA
                     GISPENFRAFSAGYGTAEDYAEIAFAQLRGPNPPSVIMVCDDLSAVDVLAGLRAKGIR
                     VPEDVAMTGFDDAFDPFIRASVHTQSNVYPWTFPLTTVRRPHRDIGRKAAQVLIEMIE
                     KQLPLEAVDYVLHNVELVIRDSSRLPERRKVSAKRILRRRTPAGANGSGSEFEEDERL
                     "
     gene            complement(1727..>2845)
                     /locus_tag="PLS90_17000"
     CDS             complement(1727..>2845)
                     /locus_tag="PLS90_17000"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPK04145.1"
                     /translation="ILLPFILHAVGGWDSLLTQLRPEQTSLVAPTEIGLFYILIVAFN
                     ALVGNITQPTVLANCAAGRTEMDGRVGYMGGSLVKRVCTIPWALTGLAAIAYFGTTPV
                     EPDLVWGRVANEFLPIMMPGLIGIFLAALFASTMSVCSALMIVASGLFTENLYRRMVR
                     QRSERHYVQVARLASFGIVVGGVIFAFVIPTVIKGIELLWIIPTMMGIAFWLGLFWRR
                     ATVAGAWAATVVGFVAWWITTQAFFVDYLAVSDMGQRLRFVSPRNGVSEIYLPWQMLF
                     YLGAGGLAGVLFSLFSRPTSEEKLDRFYELVSTPIVPGEIIRKPCELPPEVRPRPMHK
                     LIPLRNWMIPVPSAVSLAGFAVGWIFVSLLIMFVYWIA"
BASE COUNT          611 a          847 c          876 g          511 t
ORIGIN      
        1 cgagtcgctc gtcctcctcg aattcacttc cagacccgtt cgcgccggcg ggagtgcggc
       61 gccgaagaat tctcttcgcg ctaactttcc gtctttcggg aagccggctt gaatcgcgga
      121 tgactaattc cacgttgtgc aggacgtaat ccaccgcctc gagcggtagc tgtttctcga
      181 tcatctcgat cagcacctga gcggccttgc gtcctatgtc gcgatgcggg cgacggacgg
      241 tcgtcagggg gaatgtccag ggatacacgt tggactgggt atgaacgctc gcgcggataa
      301 agggatcgaa cgcatcgtcg aagccggtca tggcgacatc ttccgggacg cggatcccct
      361 tggcacgcaa cccggcgagg acatccacgg ccgaaagatc gtcgcaaacc atgatgaccg
      421 acggtggatt gggcccccgt agttgcgcga aggcgatttc ggcgtaatcc tccgcggtgc
      481 catagcctgc ggagaaggct cggaaatttt ccggactgat cccggcggcg gtcacttcgg
      541 cgcgcgctcc ggaaatgcgc gcctggaccg tctccaggct gaaatcctcg gcgctggcca
      601 gatagataaa agtccggtgg ccaacctcaa gcagatgacg cgccaggagt tggccgccgc
      661 ggtagttgtc gcactgaatc accggagcag gaaagccctg ccagcgcgtg tgcgcgaagg
      721 caatcggcag cttggtgtcg ctgatcagtt ccatcgcctg gtttacttcg ggctcattcc
      781 agatcttgtt cagcagcagt ccatcaacgc ggtgctgcag aaaagagcgg acgtaatcca
      841 gcttgagggc gccatcatag cgcgtgcagc cgatgaagca gtggtagccc aggcgattga
      901 cctcggcctc gaaagcgtcg gccacgggca accagcggta atcgagcaga ttggtcagca
      961 ggatgccgac ggtcttggtg cgtatgccgg ccaggtgcgc ggcggccatg ttgcgctggt
     1021 agttgaggcg ccgagcggcg gccataatgc ggctgcgcgt attggcgctg acgccgatgt
     1081 tattcggcga acggggattg agcacgctgg agacggcgtt gatgctgacg ccgacctcct
     1141 gggcgatatc cttaagagtg gtgcgttgct ttttcatctc gccagtttaa cggagagccc
     1201 cgagcgatgt ctgtgttaaa gacgggtagc agccggcagc ccgccactgt catgtagcgt
     1261 tatattcttg aatgaaatct acccttgagc ggggaactcg tcaagaaaaa tgtttcccct
     1321 ttttttgggg gcgaccgggc cgccctgcca actgccatac agacccattc tcctttataa
     1381 acaaaagagc caaggctgat atcgcgggca atggtccgtt ccatactctg ccattccgaa
     1441 gcagagccgc gccatccaag ccgatagata taacgatata ttcatgaatc ccaagccccc
     1501 ctcccctgat ccggcccgcg gtgacgaagc cctcgcaggc cgtacttcgc cgtcacgaaa
     1561 ttgctcgaat cgaccatggt tcccagcagc gcatagagca agccgaaggt tttgcggccg
     1621 tctttggggg caacgctggg ggggggcgac tgcctgttca tcgcgttgcg ttccgggcag
     1681 gtgcggccct ggggtgcagg ggggataacc cgcttccgcc ggaacctcat gcaatccaat
     1741 agacaaacat gatgaggagc gacacaaaga tccagccgac cgcgaaaccg gccagggaga
     1801 cggccgacgg aacgggtatc atccagtttc gcagcgggat caatttgtgc attggtctgg
     1861 gccggacttc aggcggcagt tcacagggct tgcggatgat ttctccggga acgatcgggg
     1921 tgctgacgag ctcatagaac cggtcgagct tctcctcgga agtcggccgg gagaagaggc
     1981 tgaagaggac gccggccagg cctcccgcgc caaggtagaa gagcatctgc cacggcaggt
     2041 agatctcact gactccattc cggggggaaa caaaccgcag ccgctgtccc atgtccgaaa
     2101 ccgccagata atccacaaaa aacgcctgag tggtaatcca ccaggcgaca aaaccgacta
     2161 ccgtggcggc ccacgcaccg gcgactgtcg cgcggcgcca gaacagcccc agccagaagg
     2221 cgatgcccat catcgtcggg atgatccaca gcagttcgat gcctttgatc accgtcggaa
     2281 tgacaaaagc aaagatgacg ccgccgacga cgattccaaa cgatgccagc cgagccacct
     2341 gcacataatg ccgttcggag cgctgcctga ccatccttcg gtagagattc tcggtgaaga
     2401 gtcccgacgc gacgatcatc agcgcggagc agacactcat cgtcgaagcg aacagcgcgg
     2461 cgaggaaaat accgatcagt ccgggcatca tgatcggcag gaactcgttg gccacgcggc
     2521 cccagaccaa gtccggctca accggcgtcg tgccgaaata ggcaatggcg gcaagtcccg
     2581 tcagcgccca gggaatggtg cagacgcgct tgaccagtga gccgcccatg tagccgacgc
     2641 gtccatccat ctccgtgcgg ccggcggcgc aattggccag cacggtcggc tgcgtgatat
     2701 tacccaccag cgcgttgaag gccacgatca ggatgtagaa caggccgatc tcggtgggag
     2761 cgaccagcga cgtttgctcc ggccgcaact gcgtgaggag cgaatcccaa cccccgacgg
     2821 catggaggat gaacggcagc agaat
//
LOCUS       DAOPCO010000408         2839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_27853_length_2839_cov_5.010776, whole genome shotgun sequence.
ACCESSION   DAOPCO010000408 DAOPCO010000000
VERSION     DAOPCO010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27853_length_2839_cov_5.010776"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..1000)
                     /locus_tag="PLS90_17005"
     CDS             complement(266..1000)
                     /locus_tag="PLS90_17005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020885537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPK04146.1"
                     /translation="MPESPSPALSIVVPVYNEEECLREVMEELRAALDAAGLTDWEAI
                     MVDDGSRDRSPQIARELAAADPRFRLIRFTTNCGQTAAFDMGFRSARGRLVGMMDADG
                     QNDPRDFPRLIAALEQRGVDMICGVRQKRQDSFVRRISSRIANGVRNWATQEQVTDVG
                     CSIRVFRRECFHRIKLYEGMHRFFPTLFRIEGYTIGEIPVNHRPRTRGVSKYGIHNRL
                     WRGLRDLFAVRWMQRRALRYQVRTED"
     assembly_gap    1227..1326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1358..2758)
                     /locus_tag="PLS90_17010"
     CDS             complement(1358..2758)
                     /locus_tag="PLS90_17010"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPK04147.1"
                     /translation="MRATLLIACCLMIAAVVYAATVQEQNQTGLNHLEAGRYAEAVAA
                     FDAARRQLPGNDALEQNLSYAYNAWGVSLSEAGRFREALEKLEAARRLRPEDPLLKTN
                     LTTVLVNWGTQMANQGDLTEADRLLNQATQTAAPAAQDEIRKRRSQVAFQRAQRERER
                     NETTRARRNLEEALELDPRNVHALLELADLFYQQGDTLTALDHWLAADQIDPQVPGLA
                     DRIERAFREAESEAGFESRSNRHFTVSYEGEVNDQAAEKALAILEEAYILLARELRYR
                     PERKINVVIYTHEQFATVTISPHWTGGLYDGKIRIPLPGGPLAANQEERLRAVLIHEY
                     VHALVHAMAPRAEVPSWFNEGLAMYYELPEAEQRRRAAADRNLLAELAEDDALPAVHT
                     LPTQFAAMRDQAAVEQAYRVSRGFVHWLMDRYRTPKILELLREVADGVEFNHAVETTY
                     NRTLAELHEDWRASLL"
BASE COUNT          422 a          786 c         1074 g          457 t
ORIGIN      
        1 cttttcgccc ggcatgccgc ggaccaacca gcaggtcgtc agccatgaaa gggtgatcga
       61 caggcaagag gccattacca gggtgaccgt ccgttccttc atctgagggc tccgatcggc
      121 tgccatgttc cctcgaagct gagggaatcg atggatggtt atcgtcctcg agcgcgacgg
      181 gcgagaggcc cggccccgcg accccggacc gaatcagtaa caggcgatcg ccggttgttc
      241 gcgccagtct tggatcatca cgcgttcaat cttcggtgcg aacctggtag cgcagggcgc
      301 ggcgctgcat ccagcggacg gcgaacagat cgcgcaggcc gcgccagagg cggttgtgaa
      361 tgccgtattt ggagacgccg cgggtgcgcg ggcggtgatt gacggggatt tcgccgatgg
      421 tgtagccctc gatgcgaaag agggtcggga agaagcggtg catgccttcg tagagcttga
      481 tgcggtgaaa gcactcgcgg cggaagacgc ggatcgagca gccgacgtcg gtcacctgtt
      541 cctgggtcgc ccagttgcgg acgccgttgg caatgcgcga gctgatccgg cggacgaaag
      601 aatcctggcg cttctggcgg acgccgcaga tcatgtcgac gccgcgctgc tcgagcgccg
      661 cgatcaggcg ggggaaatcg cgcgggtcgt tctggccgtc ggcgtccatc atgccgacca
      721 gccggccgcg ggcggagcgg aagcccatgt cgaaggcggc ggtctggccg cagttggttg
      781 tgaagcggat gaggcggaag cgggggtcgg cggcggcgag ctcgcgggcg atctgggggg
      841 agcggtcgcg cgagccgtcg tcgaccatga tcgcttccca gtcggtgagg ccggcggcgt
      901 cgagcgcggc gcgcagttcc tccatcacct cgcggaggca ttcctcttcg ttatagacgg
      961 ggacgacgat cgagagggcg gggctggggg attcgggcat cggtcgggcc atacggatca
     1021 cgaaacgggc gcgccggcgg gcgcgcgcac ctgacagcat aaacgcggcg acgggcgatg
     1081 ccacggaaaa aagcgcggag cggcgggggc ggcggcggga cgccgggaga gcggaatgca
     1141 gtggggcggg gctggaattt gatccggaca tggccggtat tgccgctgtg ctcaattgcc
     1201 ggcgacgccc tggcatccct ccgggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnntcag gagcgtggcg tttccaaggg cggggggtta gaggagtgag gcgcgccagt
     1381 cttcgtgcag ctcggcgagc gtgcggttgt aggttgtctc gacggcgtgg ttgaattcga
     1441 cgccgtcggc gacctcgcgc aacagctcga gaatcttcgg ggtgcggtag cgatccatca
     1501 gccagtggac gaagccgcgc gagacgcgat aggcctgctc gacggcggcc tgatcgcgca
     1561 tggcggcgaa ctgcgtgggg agcgtgtgga cggcaggcag ggcgtcgtcc tcggcgagct
     1621 cggccagcag gttgcggtcg gcggcggcgc ggcggcgctg ctcggcctcg ggcagttcgt
     1681 aatacatcgc cagcccctcg ttgaaccacg aggggacctc ggcccgcggg gccatggcat
     1741 ggacgagggc gtgaacgtat tcatggatca ggacggcgcg gaggcgttcc tcctggttcg
     1801 ccgcgagcgg cccgccgggg agcggaatgc ggatcttgcc gtcgtagagc ccgccggtcc
     1861 agtggggcga aatggtgacg gtggcgaact gctcgtgggt gtagatgacg acgttgatct
     1921 tgcgctcggg gcggtagcgc agctcgcggg cgagaaggat gtaggcttcc tcgaggatgg
     1981 caagggcctt ctcggcggcc tgatcgttga cctcgccctc atatgagacg gtgaaatggc
     2041 gattggagcg gctttcgaag ccggcctcgg actcggcctc gcgaaacgct cgctcgatgc
     2101 ggtcggccag gccggggacc tgcggatcga tctggtcggc ggcgagccag tggtccaggg
     2161 cggtgagcgt gtcgccctgc tggtagaaga gatcggcgag ttcgaggagg gcgtggacgt
     2221 tgcgcggatc gagttcgagg gcttcctcga ggttgcgccg ggcgcgggtg gtttcgttgc
     2281 gttcgcgttc gcgctgggcg cgctggaagg cgacctgtga gcgccgcttg cggatctcat
     2341 cctgggccgc cggggccgcc gtctgggtcg cctggttcag cagccggtcg gcctcggtca
     2401 ggtcgccctg gttggccatc tgtgttcccc aattgaccag gacggtggtc aggttcgtct
     2461 tgaggagggg atcctcgggt cggaggcggc gggccgcttc gagcttctcc agggcttcgc
     2521 ggaaacggcc ggcctcgctg agcgagaccc cccaggcgtt ataggcatag gagagattct
     2581 gctcgagagc gtcgttgccg gggagctggc ggcgggcggc gtcgaacgcg gcgaccgctt
     2641 cggcgtagcg gcccgcttcc agatggttga gacccgtctg attctgttcc tgcacggtgg
     2701 cagcatagac caccgcggcg atcatcaggc agcaggcaat caggagggta gctcgcatgt
     2761 ctggacaacg ggcaggcggg gttgggctgc ggggggaacc cggccgcgcg aggcgggaca
     2821 ggccggcaag gccccctgc
//
LOCUS       DAOPCO010000409         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_28023_length_2825_cov_3.870397, whole genome shotgun sequence.
ACCESSION   DAOPCO010000409 DAOPCO010000000
VERSION     DAOPCO010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28023_length_2825_cov_3.870397"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..709)
                     /locus_tag="PLS90_17015"
     CDS             complement(140..709)
                     /locus_tag="PLS90_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04148.1"
                     /translation="MMMPWILYRGQSALLLLLGVAGMAACGGGESTTEPAPAAPPAAT
                     APAGTPAALIPTPADDAPAASAPVAAAQASPAPANSREGWPTEEEAKEAIFRVEHAIH
                     ASQTNKDVWHVEDMRHEVQSVRFAERTTQKQMDYGAQAITVYPARIHYTRITTYRDKP
                     ETREELGDNGVWFLYQDSFGDWTGKFGTE"
     gene            1009..1998
                     /locus_tag="PLS90_17020"
     CDS             1009..1998
                     /locus_tag="PLS90_17020"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HPK04149.1"
                     /translation="MLNSRTLLLHLRSQWFSLAMVALGVLAWAWPDLGPALNPRNGTK
                     HLIIAAVFLSQGLGLRTEEVRRGFSQWRLHLFIQLFSFALLPLACWLALLPWRATLPE
                     GLVIGFYLLAVLPTTITSCVVFTQLAGGNTAAALFNAIAGNLAGIVLSPALLLLLIGQ
                     SAPVRPLEILIQLSLLVALPFAVGQALHWLLHARVAGWRRRLSLFNSFSILAVLYFVF
                     SESFRSGTLQSIGADSIVPLALLIPAHLAILGLSWGGGRLLRFERRDLIAILFCAPQK
                     TLALGIPLITASLAARPDLLGPATLPLLVYHPFQLFIAGLLKDWVGADRAPHA"
     gene            2203..>2825
                     /locus_tag="PLS90_17025"
     CDS             2203..>2825
                     /locus_tag="PLS90_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04150.1"
                     /translation="MFRFRSLELLAFTASLMLTRSLAAQVMQIAPDDFIISEMGEPED
                     EIAAAHNPQVAWNPVDQEYLVVWEGQESVTPTTEHGVEIYAVRINRFGQRVGDQIRVS
                     DMGPAGDTEHEASDPAVAYNPDDHEYLVLWSGDDVNSGEFEIHGQRLSAAAEPLGENF
                     RITTTDVDENTSSDAEIPRVAYNSRDQEYMVICTVDDNSASANNHYE"
BASE COUNT          432 a         1056 c          795 g          542 t
ORIGIN      
        1 gatggttctt cttttttatg cgccaccctc tgagccgtta tgaaggatta aggtgccccc
       61 ccggcggccg gcacggtcgg ccacgaccac cccgccatct ttcggacttt ctccaagtcc
      121 ccgcccgcgc gagccggccc tattccgttc cgaacttccc cgtccagtcg ccaaagctgt
      181 cctggtacag aaaccacacg ccgttgtcgc ccagttcctc gcgcgtctcc ggcttgtcgc
      241 gataggtcgt gatccgcgtg tagtggattc tggccgggta gaccgtgatc gcctgcgccc
      301 cgtaatccat ctgcttctgc gtggtccgct cggcgaaccg caccgactgc acctcgtggc
      361 gcatgtcctc gacgtgccac acgtccttgt tggtctgcga ggcatggatg gcgtgctcga
      421 ccctgaagat cgcctccttg gcctcttcct cggtcggcca gccttcgcgg ctgttggcgg
      481 gcgcggggct ggcctgagcc gccgcaacgg gagccgacgc cgccggcgcg tcatcggccg
      541 gcgtcgggat cagcgcggcc ggtgtgccgg ccggcgcggt cgccgcgggc ggggcggccg
      601 gcgccggttc ggtcgtgctt tcgccgccgc cacaggccgc catgcccgcc acgcctagca
      661 gcagcagcag tgccgactgt ccgcgataga gaatccaagg catcatcatc acctcaaaac
      721 cacgacggcg cgtggatcgc ttgcgaaggg gagtgagtgc tccccttccg cggcagcgag
      781 cggggtattc cgcttgcccg cacggttgaa aaaggcagac agcaaaaaga cggggtgtga
      841 gtggggtacc cttatgatac gttgcgttac cacatggccg gccacaggga agggtcccgg
      901 aaggcaacaa cttcccacca cgatcctccg tctgtcaacg gcgattccaa acccgatgcg
      961 catgtcatcg ttccggccgc acccctggtc ggccgcgcac ccctcacgat gctgaactcc
     1021 cgaacgctcc tccttcatct ccgctcgcag tggttctcgc tggcgatggt cgccctcggc
     1081 gtgctggcct gggcctggcc ggatctcgga cccgcgctca acccccgcaa cgggaccaag
     1141 catctcatca tcgccgccgt cttcctcagc cagggactcg gcctgcgcac cgaagaagtc
     1201 cgccgcggct tcagccagtg gcgccttcat ctcttcattc agctcttttc gttcgccctc
     1261 ctgccgctcg cctgctggct cgcgctgctg ccgtggcgcg cgacgctgcc cgagggcctc
     1321 gtcatcggct tctacctcct cgccgtcctg cccacgacca tcacctcctg cgtcgtcttc
     1381 acccaactgg ccggcggcaa caccgccgcc gcgctgttca atgccatcgc cggcaacctc
     1441 gccggcatcg tcctttcccc cgccctcctg ctcctgctga tcggccagag cgcccccgtc
     1501 cggccgctcg agatcctcat ccagctctcg ctgctggtgg cgctgccgtt cgcggtcgga
     1561 caagccctcc actggctgct gcacgctcga gtcgccggct ggcgccgcag gctctccctg
     1621 ttcaacagtt tctccatcct cgccgtcctc tacttcgtct tcagcgaatc gttccgctca
     1681 ggcaccctgc agagcatcgg cgcggactcc atcgtccccc tggccctgct gatcccggcc
     1741 catctggcga tcctcgggct ctcctggggc ggcggccgcc tgctgcggtt cgaacggcgc
     1801 gacctgatcg cgatcctctt ctgcgcgccg cagaaaaccc tcgccctcgg catcccgctc
     1861 atcaccgcca gcctggccgc ccgccccgac ctgctcggcc ccgccacgct ccccctgctc
     1921 gtctaccatc cgttccagct cttcatcgcc ggcctgttga aggactgggt cggcgcggac
     1981 cgcgctccgc acgcctgacc ccgccgcgaa cgctccaccc gcggagcgcg actgaatcgc
     2041 gcggggatcg gccacgccgc atcgaattcc gcattacttg cttcgacggt ccttaacttg
     2101 ctaacatttt tcaaatcaaa tccgcgtctt cctgcgtatc cattcccgct tcgggaacgg
     2161 ctggcttgga tcatgaggcg actcacgaaa ggcaggcacg ccatgtttcg gttccgctcc
     2221 cttgagctgc tcgctttcac cgccagcctg atgctgacgc gcagcctcgc cgctcaggtg
     2281 atgcagatcg cccccgacga cttcatcatc agcgaaatgg gtgaacccga ggatgagatc
     2341 gccgcggctc ataacccgca ggttgcctgg aatccagtcg atcaggaata cttggtcgtc
     2401 tgggaggggc aggagtcggt caccccgacg accgagcacg gggttgaaat ctacgcggtc
     2461 cgcatcaacc gcttcggcca gcgcgtcggc gatcagatcc gcgtcagtga catgggcccc
     2521 gccggcgaca ccgagcacga ggcctctgat cccgccgtcg cctacaatcc cgacgatcac
     2581 gaatatctcg ttctgtggtc gggtgatgac gtcaactccg gcgaatttga aatccatggc
     2641 cagcgcctgt cggccgcggc tgagcccctt ggcgaaaatt tccgcatcac cacgaccgac
     2701 gtcgacgaga acacctcctc cgatgccgag attccccgcg tcgcttacaa ctcacgcgac
     2761 caggagtata tggtcatctg caccgtcgat gacaacagcg cctccgcgaa caatcattat
     2821 gaaat
//
LOCUS       DAOPCO010000410         2805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_28300_length_2805_cov_4.125818, whole genome shotgun sequence.
ACCESSION   DAOPCO010000410 DAOPCO010000000
VERSION     DAOPCO010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28300_length_2805_cov_4.125818"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1264)
                     /gene="dxr"
                     /locus_tag="PLS90_17030"
     CDS             complement(83..1264)
                     /gene="dxr"
                     /locus_tag="PLS90_17030"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPK04151.1"
                     /translation="MASNRKRLCVLGSTGSIGETTLAVVRDYADRFQVVGLSAHAAAD
                     ALAVQAREFGVEHVCLTGNGNGGELEALRPLRGEAGLVELIDRCEPDMVVVATVGAAG
                     LPPTLRAIERRIPIALANKEVLVTAGDLVMRRARRLDVPILPIDSEHNAIFQCLGGVS
                     DARRLPLRRIILTASGGPFRGRSRAELSGVTREEALNHPTWNMGRKITIDSATLMNKG
                     FEVIEVHHLFGVETARIEVIIHPQSVIHSLIEYHDGSMLAQMGVTNMYLPIANVLAYP
                     ERLENARFAPLDLAAVATLTFEAPDLHAFPCLRYAYEAVEAGQTYPTVLNAANEIAVR
                     RFLEGEIAYLGIPALIDAALQAHRPQPADELEAIAEADRWARAYCESRAPMEPTAERM
                     P"
     gene            1614..2711
                     /locus_tag="PLS90_17035"
     CDS             1614..2711
                     /locus_tag="PLS90_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK04152.1"
                     /translation="MLQKLRRLTVKLSRSGPTISRPFVFGVVGINHPHIYHQVKVLRE
                     AGGTLQSFFAPEDDLAARFQKKYPHASRARAEAEVLEDESISVIASAAIPADRAAIGL
                     RAMRHGKDVLMDKPGATTLDQVTELRRAHQQTGRFYTVLFSERLLTKSTVRALELTRS
                     GAIGRVLHTLGTGPHRPALATRPDWFFNKQRYGGILVDIAAHQIDQFLTFTNSTEARI
                     VAAQVGNLNHPEYPELEDFGDLTLHGNGGLGYIRVDWFTPAGLPVWGDSRLFILGTEG
                     TIEVRKYVDVGGRPGADHLFIVDAESARYEDCSRVELPFPREYGVDLAQRTQNAISQE
                     HVFRAMELAIRAELQATVVTPPQSGRALEKR"
BASE COUNT          472 a          990 c          876 g          467 t
ORIGIN      
        1 gcccttcgtc cctcgtcctt ggctctcgaa aatctgttgt tctgtccttc cgttgtgaaa
       61 cgcgcctgct gagcccgagg gttcagggca ttctctccgc tgtcggctcc atcggcgccc
      121 ggctctcgca gtatgcccgc gcccagcggt cggcctcggc gatcgcctcc agctcgtcgg
      181 ccggctgcgg gcggtgcgcc tgcagcgctg catcgatcaa cgccggaatc cccaggtagg
      241 cgatctcccc ctccaggaac cggcgcacgg cgatctcgtt cgcggcgttc aacaccgtcg
      301 ggtaggtctg ccccgcctcc accgcctcgt aggcgtagcg caggcaggga aaggcgtgca
      361 ggtcgggcgc ctcgaacgtc agcgttgcca cggcggccag atcgagcggc gcgaagcgcg
      421 cgttctccag ccgctccgga taggccagca cgttggcgat cggcagatac atgttcgtca
      481 cgcccatctg cgccagcatc gaaccgtcgt ggtactcaat caggctgtgg atgacgctct
      541 ggggatggat gatcacttcg atccgcgcgg tctcgacgcc gaacaggtga tgcacctcga
      601 ttacctcgaa ccccttgttc atcagcgtcg ccgagtcgat cgtgatcttg cgccccatgt
      661 tccaggtcgg gtgattgagc gcctcctcgc gcgtcacgcc gctcagctcg gcgcggctgc
      721 gcccgcggaa cggcccgccc gaggccgtca ggatgatgcg gcgcagcggg agccggcgcg
      781 cgtcggacac gccgcccagg cattgaaaga tggcgttgtg ctcgctgtcg atcggcagga
      841 tcgggacgtc gagccggcgc gcgcggcgca tcaccagatc gccggctgtt accaaaactt
      901 ccttgttcgc cagcgcgatc gggatccgcc gctcaatcgc gcgcagcgtc ggcggcaagc
      961 ccgccgcgcc gacggttgcc acgacgacca tgtcgggttc gcagcgatcg atcagctcga
     1021 ccagtcccgc ctcgccgcgc agcgggcgca gcgcttccag ttcgccgccg tttccgttgc
     1081 cggtcagaca aacgtgctcg acgccgaatt cgcgcgcctg caccgccagc gcgtccgcgg
     1141 cggcgtgggc cgacaggccg acgacctgga accggtcggc gtaatcccgc accaccgcga
     1201 gcgtcgtctc gccgatcgaa ccggtcgagc cgaggacgca cagacgtttt cgattggatg
     1261 ccatgcagtc ggcgtcatgc agccaggcgg ggtgggacgc gagcggcttg ccgtcggacg
     1321 gatgcagccc acggtctcca caaggcgggg tggcgcgaca aaccctgctc cagcttaagg
     1381 aaaccggccg agaagggtca attccttttg gctccgtccg ccgggtccac cccgtccatc
     1441 ttctcgggcc cccggcccgg cggtttggtt ttcatggcgc gccacgcggc aattcgctag
     1501 cctcaagcca acatatcgcc tcgactccgc caatacgcac tcccggcgtt ccggcctcgt
     1561 ccaaaacatc acgatccaca tgggatcgac gcacccgtcg agcgatgagc gccttgcttc
     1621 agaaactgag gagactaacc gttaaactgt caaggtccgg accgaccatc tcccggccct
     1681 tcgtcttcgg cgtcgtcggc atcaatcacc cgcatatcta tcatcaggtg aaggtcctgc
     1741 gcgaagccgg gggaacgctc cagtcatttt tcgcccccga ggacgatctg gccgcccgct
     1801 ttcagaaaaa atatccgcac gcgagccgcg cccgcgcgga ggccgaggtc ctcgaggacg
     1861 aatcaatctc cgtcatcgcc agcgcggcga tcccggccga ccgcgccgcc atcggcctgc
     1921 gcgcgatgcg ccacggcaag gacgtcctga tggacaagcc gggcgccacg acgctcgacc
     1981 aggtgaccga gctgcgccgg gcccaccagc aaacagggcg cttctacacc gtcctgttca
     2041 gcgaacggct gctcacgaag tccaccgtgc gggcgctgga gctgacccgg tcgggcgcta
     2101 tcgggcgcgt cctgcacacg ctcggcacgg ggccgcatcg cccggcgctc gccacccggc
     2161 ccgactggtt cttcaacaag caacgctacg gcggcatcct cgtcgatatc gccgcgcatc
     2221 agattgacca gttcctcacc ttcacgaatt cgaccgaggc gcggatcgtc gccgcacagg
     2281 tcggcaatct gaatcatccc gagtatcccg aactggagga tttcggcgac ctcacgctgc
     2341 acggcaacgg cggcctgggc tacatccgcg tcgattggtt cacgccggcc gggctgccgg
     2401 tgtggggcga ttcacggctg ttcatcctcg gcaccgaggg gacgatcgag gtccgcaagt
     2461 atgtcgatgt cggcggccgc cccggcgccg accatctctt catcgtggat gccgagagcg
     2521 cccgctacga ggactgctcc cgcgtggaac tcccgtttcc ccgtgagtac ggcgtggatc
     2581 tggcccagcg cacacagaac gcgatcagcc aggaacacgt cttccgcgcc atggaactgg
     2641 ccatccgcgc cgaattgcag gcgacggtgg tcaccccgcc gcagtccggg cgcgcgctgg
     2701 aaaagaggta atacccctaa aagagggtaa taataggggt aataacggaa tccggcgcgg
     2761 ccgcgcgcgc cgactacagg tagcgcagcg actgcgcttt gaaca
//
LOCUS       DAOPCO010000411         2798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_28381_length_2798_cov_4.161502, whole genome shotgun sequence.
ACCESSION   DAOPCO010000411 DAOPCO010000000
VERSION     DAOPCO010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28381_length_2798_cov_4.161502"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(504..>2798)
                     /locus_tag="PLS90_17040"
     CDS             complement(504..>2798)
                     /locus_tag="PLS90_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04153.1"
                     /translation="NQRPLVVSASGPARFDYPLGGSTHVDVPLSLQLDDFESPTLDVE
                     VEIFDLIANGFLPAQILQDVPRDSLQMRTISLPKAPPNDANPQFTIRVRAIDTTALNG
                     GDVHSQSEWSLPFNIMLNEIPRVTAAAAPPVVNYQGDPALVDIPITLTAQDKESATLQ
                     VFATAVATSGETYPDVYLGDVQRGVSTMLPFGLPPLNAPHTYNITVRVEDGHMVSSEP
                     SAVLPVRLNRPPVAELVTPYLGQVFLFTPPMTDIPYTILLHDDWSDDLVVHFAADGID
                     DVNQHSDPAFTSGVPQEKSGQITTTSSGDKSMTFTVTDEDGNATQGVVDFRLHQKPVV
                     EEILTPENQAFPFNGETTMITLDLRVSDDFSDPIQVLFDIDNNGEFDLDNTTNLPNDV
                     NASAVLTYPFNAWGFYTIRVVAVDEYGVHSDPVLRTIRLNAWPTVILHTPEAGSAPIL
                     EQNLAPIPFRLQVLEDFDADVRIAVDIQTNGTSTTQIIDNVAVDGIQTVPVSFAQWGP
                     TTIQTYAIDDNGYMSRTPDVRDILVNDRPQVMITQPTGEPQIRIDVPQNGTKAVANIN
                     FDATFSDTFGGALTHIWYFGDLTPDFYQAPPIMHAFENFRDYVVEYQVVDSRDAKGTD
                     TLLVAINDEPSLSLKVFPAIFVPDTNTWRIERNRPTTFSSHGENIYRDQDEELVLERD
                     LNSSDDPGMKLLDTKSAPLPGYTQFIDGVETLTFADLGDFTYALTITERFDGQPVTTT
                     GSWTIRVEEKIPELNQAEDWELYE"
BASE COUNT          464 a          742 c         1066 g          526 t
ORIGIN      
        1 gcgggggggc cgaaccggcg gcgggagaag ccgaagccgc gcagtagccg acgcctgatc
       61 ccgtgagggg gggggtgtgg gtgtggtcga gctggccggg ccggcagagc ctatcattga
      121 tgaagttgag ttgcctgaaa gcgaaccgag aggtcggttc gcttttttaa ttccgtggaa
      181 atcagcagga acgctccagc ccagctttcc atcgcccccg cacgcgaggc cacgcggaga
      241 gctgggcgag gatcttgata cgtccccgtg gacggaaggc gagcagctcc ggccccctct
      301 tgcaggggtt tgggcggcga cgcagggttt cggcctgcgg cctttaccct gcgcttgatt
      361 ccggccatcc gctccgcgga cttgagcgat tgtcgcccgg ttggacttga agcgtttgtc
      421 gcgcggccgg agccgaatgg aaaacgggcc ggtcggggat agccgaccgg cccggggtga
      481 aggcgcgcgg aattgcgaac gggctactcg tagagctccc aatcctcggc ctgattaagt
      541 tcgggaatct tctcctcgac gcggatggtc cagctcccgg tcgtcgtgac cggctggccg
      601 tcgaaccgtt cggtgatcgt gagggcgtag gtgaagtcgc ccaggtcggc gaaggtcagg
      661 gtttcgaccc cgtcgatgaa ctgggtgtaa ccgggcaacg gcgcactctt cgtatcgagg
      721 agcttcatgc ccgggtcgtc cgaggagttc aggtcgcgct ccagcacgag ctcctcatcc
      781 tgatcgcgat agatattctc gccgtgggag gagaaggtgg tcgggcggtt gcgctcgatg
      841 cgccaggtgt tggtgtcggg gacgaagatg gcggggaaga ccttcagcga aagcgagggt
      901 tcgtcgttga tcgccacgag cagggtgtcg gtgcccttgg cgtcgcggct gtcgacgacc
      961 tggtactcca ccacgtagtc cctgaagttc tcaaaggcgt gcatgatggg gggcgcctgg
     1021 tagaagtcgg gggtcaggtc gccgaagtac cagatatggg tcagcgcgcc gccgaacgtg
     1081 tcgctgaagg tcgcgtcgaa gttgatgttg gcgacggcct tcgtgccgtt ctgcgggaca
     1141 tcgatgcgga tctgaggctc gccggtcggc tgcgtgatca tgacctgcgg acggtcgttg
     1201 acgaggatgt cgcgcacgtc gggcgtccgc gacatgtagc cgttgtcgtc gatggcgtag
     1261 gtctggatcg tcgtcggccc ccactgggcg aagctgaccg ggacggtctg gatgccgtcg
     1321 acggcgacgt tgtcgatgat ctgggtcgtg ctggtgccgt tggtctggat gtcgacggcg
     1381 atgcgcacat cggcatcgaa gtcctcgagc acctggaggc ggaaggggat cggggcgagg
     1441 ttctgttcga ggatcggcgc ggagccggcc tcgggcgtgt ggaggatcac ggtcggccag
     1501 gcgttgaggc gaatggtgcg gaggaccgga tcgctgtgga cgccgtattc gtcgacggcg
     1561 acgacgcgga tcgtgtagaa gccccaggcg ttaaacggat aggtcaggac ggccgaggcg
     1621 ttgacgtcgt tgggcaggtt ggtcgtgtta tccagatcga actcgccatt gttgtcgatg
     1681 tcgaagagga cctggatcgg gtcgctgaag tcgtcgctga cgcgcaggtc gagggtgatc
     1741 atcgtcgttt ccccgttgaa ggggaatgcc tggttttcag gagtcaggat ctcctcgacg
     1801 acgggcttct ggtggagccg gaaatcgacg acgccctggg tggcgttgcc atcctcgtcg
     1861 gtgacggtga aggtcatcga cttgtcgccg gaggaggtgg tggtgatttg tcccgacttc
     1921 tcctgcggga cgcccgaggt gaacgccggg tcgctgtgct ggttgacgtc gtcgatcccg
     1981 tcggcagcga agtggacgac cagatcatca ctccagtcat cgtgcaggag gatggtgtag
     2041 gggatatcgg tcatgggcgg agtaaagagg aagacctggc ccagataggg ggtgacgagt
     2101 tcggcgaccg gggggcggtt gaggcgcacg ggcagcacag cggagggctc gctgctgacc
     2161 atgtggccat cctcaacccg gacggtgatg ttgtaggtgt gcggagcatt gagcgggggg
     2221 aggccgaacg gcagcatcgt cgagacgccg cgctgcacat cgcccagata aacgtcgggg
     2281 taggtctcgc cgctggtggc gacggccgtg gcgaagacct gcagcgtggc cgactcctta
     2341 tcctgcgcgg tgagggtgat cgggatgtcg accagcgcgg ggtcgccctg gtagttgacg
     2401 acggggggcg cggcggcggc ggtgacgcgc gggatttcat tcagcatgat gttgaacggc
     2461 agggaccact cgctctggga gtggacatcg ccgccgttga gggcagtcgt atcgatcgcg
     2521 cgcacgcgga tggtgaactg gggattggca tcgttgggcg gcgccttcgg caggctgatg
     2581 gtgcgcatct ggagcgaatc gcgcggaacg tcctgcagga tctgggccgg gaggaaccca
     2641 ttggcgatca gatcgaagat ctcgacctcg acatccaggg tcggcgattc gaagtcgtcg
     2701 agctggagcg acagggggac gtcgacgtgg gtcgatcccc ccagcgggta gtcaaagcga
     2761 gcggggccgg aggccgaaac gaccagcggc cgctgatt
//
LOCUS       DAOPCO010000412         2788 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_28526_length_2788_cov_2.189535, whole genome shotgun sequence.
ACCESSION   DAOPCO010000412 DAOPCO010000000
VERSION     DAOPCO010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28526_length_2788_cov_2.189535"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PLS90_17045"
     CDS             complement(<1..942)
                     /locus_tag="PLS90_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPK04154.1"
                     /translation="MTGRIVAVFGNMVVAETSGRVVQNSIGYCCRDDGAHLLSEVIRI
                     RGRLADLQVFEETRGLKVGDTVEFKEQMLSVVLGPGLLGQIYDGLQNPLPQLADKAGF
                     FLKPGEYLHGLDTARKWEFTPLAKPGDTVCAGDALGEVPEGIFKHQIMAPFAWGGEFR
                     VESVVGAGQYTVEQEIAVLAGPDGQKRPVTMQQEWPVKRALNVSRRRLMPTEPLVTRV
                     RIIDSMFPIVRGGTYCIPGPFGAGKTVLQQITSRHAEVDIVILAACGERAGEVVETIR
                     EFPELLDPRTGKSLMERTIIICNTSAMPVAAREASVYT"
     gene            complement(953..1687)
                     /locus_tag="PLS90_17050"
     CDS             complement(953..1687)
                     /locus_tag="PLS90_17050"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPK04155.1"
                     /translation="MAGNYYLLTSLPTLDALGAEPPMSAAEFAERVADADGPAEIVRA
                     ILLGDDLLQRDAFLAGELDQPSPAVLTPAQARNEQPLPEFLAASGAPEDSHTQRSTTD
                     RLWASYFRHAAGLAATRRNAFLSEWVRHEVGLRNALATARAKALDLNATDYLAAPELG
                     LDAAGFEETVGEWAGATDLVAAQRVLDAARWQWITSHDAWFGFGDDELAAYAAKLSLM
                     ERWRRLAEAQREQPDDTDRFATQADQ"
     gene            complement(1701..2339)
                     /locus_tag="PLS90_17055"
     CDS             complement(1701..2339)
                     /locus_tag="PLS90_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04156.1"
                     /translation="MAETIESFVSKLQKDGVEAGRQEAEKIKAEASQAAEKLLADAKK
                     QAEQIVADAEKQAKSLQDRSRTELELAARDIVLKLRDALGRAIGAVLAAGAGQKLQDV
                     EFLGSMLHDLILKYAEADIEHGGRIQIDVPEEIRQKLVDWALREMGQDKLEGLKISLD
                     LKGTLAQAGFEYNLTGATVEVTLESVVEMLTKMVSPAMRELLDRAMASGDDK"
     gene            complement(2514..>2788)
                     /locus_tag="PLS90_17060"
     CDS             complement(2514..>2788)
                     /locus_tag="PLS90_17060"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HPK04157.1"
                     /translation="THDLPNGLAVRLEAPEDWRVKQIAFREGLTDTEARLRVRAREQE
                     REYLRKIYEARFPRKPAFNLVYDCSVFPLSTIAQHVVQALKLLKII"
BASE COUNT          419 a          935 c          932 g          502 t
ORIGIN      
        1 cgtgtagacc gacgcctcgc gggcggccac cggcatcgcc gacgtgttgc agatgatgat
       61 cgtccgctcc atcaggctct tgcccgtgcg cgggtcgagg agttccggga actcgcggat
      121 ggtctcgacc acctcgcccg cgcgctcccc gcaggcggca agaatgacga tgtccacctc
      181 ggcgtggcgc gaggtgatct gctggaggac ggtctttccc gccccgaacg ggccggggat
      241 gcagtacgtc ccgccgcgga cgatggggaa catcgagtcg atgatccgca cgcgggtcac
      301 cagcggctcg gtgggcatca ggcggcggcg ggagacgttc agcgcccgct tgaccggcca
      361 ttcctgctgc atggtcacgg ggcgcttctg cccgtcgggc ccggcgagga cggcgatctc
      421 ctgctcgacg gtgtattgcc ccgcgccgac gaccgattcc acgcggaact ccccgcccca
      481 ggcgaacggc gccatgatct gatgcttgaa gatcccctcc ggaacctcgc ccagcgcgtc
      541 gcccgcgcaa acggtgtcgc cgggcttggc gagcggcgtg aactcccact tgcgggcggt
      601 gtccagcccg tggaggtatt cgcccggttt gaggaagaag cccgctttgt ccgccagttg
      661 cggcaggggg ttctggaggc cgtcgtaaat ctgccccagc aggcccgggc ccagcaccac
      721 cgagagcatc tgctccttga actcgacggt gtctccgacc ttcagcccgc gggtctcctc
      781 gaagacctgg aggtcggcca gccgcccgcg gatgcgaatg acctcgctga ggagatgcgc
      841 accgtcgtcg cgacagcagt agccgatgga gttctgcacg acccggccgc tcgtctccgc
      901 gaccaccatg tttccgaaca ccgctacgat tcttcccgtc acgtttcggt cctcactgat
      961 ccgcctgtgt cgcgaatcga tccgtgtcgt caggttgttc ccgctgcgcc tcggcgaggc
     1021 ggcgccatcg ctccatcagc gacagtttcg cggcgtaggc ggccaactcg tcatcgccga
     1081 agccgaacca tgcgtcgtgc gacgtgatcc actgccatcg cgccgcgtcg aggacgcgct
     1141 gcgcggcgac gaggtcggtc gcgcccgccc attcgccgac ggtttcctcg aaccccgccg
     1201 catcgagccc cagctccggg gcggccaggt aatcggtcgc gttcaggtcc agcgccttgg
     1261 cccgcgccgt cgccagggcg ttgcgcaggc ccacttcatg tcgcacccat tccgacagaa
     1321 acgcgttgcg ccgcgtcgca gccaggccgg cggcatggcg gaaatagctc gcccacaggc
     1381 ggtcggttgt cgagcgctgg gtgtgcgaat cttccggcgc gccggacgcg gcgaggaatt
     1441 ccggcagggg ctgctcgttg cgcgcctggg cgggcgtgag caccgccggt gacggctgat
     1501 cgagttcccc ggccaggaat gcgtcccgct ggagcaggtc atcgcccagc aggatcgcgc
     1561 ggacgatctc agcgggccca tcggcgtctg cgacgcgctc ggcgaactcc gcggcggaca
     1621 tcggcggctc ggctcccagg gcgtccaggg tcggcagcga ggtcaggagg tagtagttcc
     1681 ctgccatcgg gggtgcgttc ctacttgtcg tccccggacg ccatcgcgcg gtccagcagt
     1741 tcccgcatgg ccggggagac catcttcgtg agcatctcga cgaccgattc cagggtcacc
     1801 tcgacggtgg cgcccgtgag gttgtactcg aatcccgcct gggccagcgt gcccttgagg
     1861 tcgagggaga tcttcagtcc ttcgagcttg tcctgcccca tctcccgcag ggcccagtca
     1921 acgagcttct ggcgaatttc ctcgggcacg tcgatctgga tgcgcccgcc atgctcgatg
     1981 tccgcctcgg cgtatttgag aatcaggtcg tgcagcatcg agccgaggaa ttcgacgtcc
     2041 tggagcttct gtccggcgcc ggcggcgagg acggccccga tggcgcgtcc gagcgcgtcg
     2101 cgcagtttca gcacgatgtc gcgggcggcc agttccaatt ccgtgcggga gcggtcctgg
     2161 aggctcttgg cctgcttctc ggcgtcggcg acgatctgct ccgcctgttt cttggcatcg
     2221 gcgagaagct tttccgcggc ttgggaagcc tcggccttga ttttttcagc ctcttgtctg
     2281 ccggcctcca caccgtcttt ctgaagcttg gacacaaacg attcgatcgt ctcagccatg
     2341 gggggctcca caatgaaacc acagtttgta gtgcctaccg ttgggtcggt ggcaggatac
     2401 ggcgaagttt ccacttatcg caggaacagg gccgaatttc aagggcaatt gcgaagacgc
     2461 ccctccggcg aggggggcaa cgaccggtgg caaggggagg ggaaaaccgc ccgtcagatg
     2521 attttcagca gcttcagcgc ctgcacgacg tgctgggcga tcgtggacag cgggaaaacg
     2581 ctgcaatcat acaccaggtt gaacgcgggc ttgcggggga agcgtgcctc gtaaatcttg
     2641 cggaggtatt cgcgctcctg ctcccgggcg cgaacccgca ggcgggcctc ggtgtcggtg
     2701 agcccttcgc ggaacgcgat ctgcttgacg cgccagtctt ccggcgcctc caggcgcacc
     2761 gccagcccgt tgggcagatc gtgggtgg
//
LOCUS       DAOPCO010000413         2785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_28560_length_2785_cov_4.315751, whole genome shotgun sequence.
ACCESSION   DAOPCO010000413 DAOPCO010000000
VERSION     DAOPCO010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28560_length_2785_cov_4.315751"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /gene="lpxB"
                     /locus_tag="PLS90_17065"
     CDS             complement(<1..1164)
                     /gene="lpxB"
                     /locus_tag="PLS90_17065"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPK04158.1"
                     /translation="MSPQAEIFIVAGELSGDLIASLLVKRLRTLQPDIKITGLGGERM
                     LEAGVDLRFNLVRDLAIIGFAEVVSKFPQIRRVFKETVAYLERERPRVLILIDYPGFN
                     IRLAEQAHRLGIQVLYYVCPQVWAWHRSRIHKLRKYVDQALVILPFEENFLSQGGVNA
                     RFVGTPWLDMMKLTMTREEVFAHFGFAPEKKLVGLLPGSRAGEVERLLPIILEAAEKI
                     VAERPDTQFVIPRATTVKRELIDHLLTMARVPVKVIDAYRYNVRSAMDMAIVASGTAT
                     LETGLLGCPMVIVYKVSYLSWLIGKSLVNIPYIGLINIVAGDMVVPELLQDQCTPQNI
                     AEQCLKVLGDPKEIEKVKYQLARVKEKMGGPGASDRVARIVLAMLRGEDDPAAA"
     gene            complement(1148..2134)
                     /locus_tag="PLS90_17070"
     CDS             complement(1148..2134)
                     /locus_tag="PLS90_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPK04159.1"
                     /translation="MQVIRVGVVGVGHLGQHHARNYSEMEGCQLVGVADIDHKAATRV
                     ASAHRCQAFFDFRQLIGQVDAVSVVVPTVQHYEVARAFLEAGIHVLVEKPITSTIVEA
                     RALIDIARRRNLILQVGHIERFNAAIIKLQEVLTRPGFIESHRLGPYDPRVRDVGVVL
                     DLMIHDIDIILQIVRSPIVSIDAVGVPILSPREDIANARIKFQNGCTANLTVSRVTPN
                     RMRKIRIFQPNTYVSVDYMKQAMEIYQTVEIKDAREGEPQAQIVRKRLRLKKEEPLRR
                     ELVHFVSCVREGSQPAVTGEHGQNALEVAIRVVEQIQQRQALESLSEHEPAS"
BASE COUNT          494 a          794 c         1020 g          477 t
ORIGIN      
        1 ggcggcggcc gggtcgtcct ccccgcgcag catcgccagg acgatccggg cgacgcggtc
       61 gctcgcgccc ggtccgccca tcttctcctt cacgcgggcg agctggtatt tcaccttctc
      121 gatttccttc gggtcgccga gcaccttcag acactgttcg gcgatgttct ggggcgtgca
      181 ttgatcctgc agcaactcgg ggacgaccat gtcgcccgcg acgatgttga tcaggccgat
      241 gtaggggatg ttgacgaggc tcttgccgat cagccaggaa agataggaga ccttatagac
      301 gatgaccatc gggcagccga gcaggccggt ctcgagcgtg gcggtgccgg aggcgacgat
      361 cgccatgtcc atcgcgctgc gcacgttgta gcggtaggca tcgatgacct tgacggggac
      421 gcgagccatc gtgagcaggt gatcgatcag ttcgcgcttg acggtggtgg cgcgcgggat
      481 gacgaactgc gtgtcggggc gctcggcgac gatcttctcg gccgcctcga ggatgatcgg
      541 cagaaggcgc tcgacctcgc ccgcgcggct gcccggcagc aggccgacga gctttttttc
      601 cggggcgaag ccgaagtggg cgaagacctc ctcgcgggtc atcgtcagct tcatcatgtc
      661 gagccagggg gtgccgacga accgcgcgtt gacgccgccc tgcgagagga agttctcctc
      721 gaaggggagg atgaccagcg cctgatcgac atatttgcgc agcttgtgaa tccggctgcg
      781 gtgccaggcc caaacctgcg gacagacgta gtagaggacc tggatgccga ggcggtgggc
      841 ctgctcggcc aggcggatgt tgaacccggg atagtcgatg aggatcagga cgcgtggccg
      901 ctcgcgttcc agataggcga cggtctcctt gaagacgcgg cggatctggg gaaacttgct
      961 gacgacttcg gcgaagccga tgatggcgag gtcgcggacg agattgaagc gcaggtcgac
     1021 gccggcctcg agcatgcgtt cgccgccgag gccggtgatt ttgatgtcgg gttggagcgt
     1081 gcgcaggcgc ttgacgagga gcgaggcgat caggtctccc gacagctcgc cggcgacgat
     1141 gaaaatctca gcttgcgggc tcatgctcgc tcaacgactc cagcgcctgg cgctgctgaa
     1201 tctgttcgac gacgcggatc gcgacctcca gggcgttctg gccgtgctcg ccggtgacgg
     1261 ccggctggct cccctcgcgg acgcaggaga cgaagtgaac cagctcgcgg cgcagcggtt
     1321 cctccttttt caagcgcagc cgcttgcgga cgatctgcgc ctgcggctcc ccctcgcggg
     1381 cgtccttaat ctcgaccgtc tggtagatct ccatcgcctg cttcatgtaa tcgaccgaaa
     1441 cgtaggtgtt gggctggaag atgcggatct tgcgcatgcg gttgggggtg acgcggctga
     1501 cggtcaggtt ggcggtgcag ccgttctgga acttgatgcg ggcgttggcg atgtcctcgc
     1561 gcggcgagag gatcgggacg ccgacggcgt cgatcgagac gatcggcgag cggacgatct
     1621 gcaggatgat gtcgatgtcg tggatcatca ggtcgaggac gacgccgacg tcgcgcacgc
     1681 gcgggtcgta ggggcccagg cggtggcttt cgatgaagcc ggggcgggtg aggacctcct
     1741 ggagcttgat gatcgcggcg ttgaagcgct cgatgtggcc cacctggagg atcaggttgc
     1801 ggcggcgggc gatatcgatc agcgcgcgcg cctcgacgat cgtcgaggtg atcggctttt
     1861 cgacgaggac atggatgccg gcctcgagga atgcgcgcgc cacttcgtag tgctggacgg
     1921 tggggacgac gacggagacg gcgtcgacct ggccgatgag ctggcggaaa tcgaagaagg
     1981 cctggcagcg gtgtgcgctg gcgacgcggg tggcggcctt gtggtcgatg tcggcgacgc
     2041 cgaccagttg gcagccttcc atttcgctgt agttgcgggc gtgatgctga ccgaggtggc
     2101 cgacaccgac cacgccgaca cggatgacct gcatgggaga acacgcaccc cggttgactc
     2161 cgccggtcca cgagcgacgg cggggggacg gcgggtcgga gcggatcggc cgccgccggc
     2221 cggacccacc aagacgccgc attctcaggc aagcgccaaa gggaagcaac cggaaaaagg
     2281 ggcggcaggg ggggaggtcc gcgaggattc cgcgggtatt gcacgcgtcg cgttgggggt
     2341 attccgtgag gacaattggg gaaccgtgga gttaatcgag gttcggagat gaggacgagg
     2401 gacgatttta gcatgaggac ggaaaaaatg agatttagat gttgaaattg agcgggggag
     2461 ggcagaagat acttcttgac cgtaaggtcc gaatctcggg ccagccgagg cagctcggac
     2521 ggaatcggcg ccgcgggggg ctgaaacgac cgagcgggtg gctccgagcc gacacaccga
     2581 cgcgcatact tcaacgggag gcgatgtttc catgagcgag gacaaactca agcaactgcg
     2641 catctgcatc gagcgcggca aaggcacgca ggcggccccc tatccgccgg atatgaagga
     2701 tcagccgggg gcgcgcgagc tgaccgagca actgctggcg tccttcagcg gcgcacagtt
     2761 gctgactggc cgtcacggcg cagcc
//
LOCUS       DAOPCO010000414         2703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_29656_length_2703_cov_3.626133, whole genome shotgun sequence.
ACCESSION   DAOPCO010000414 DAOPCO010000000
VERSION     DAOPCO010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29656_length_2703_cov_3.626133"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLS90_17075"
     CDS             complement(<1..531)
                     /locus_tag="PLS90_17075"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPK04160.1"
                     /translation="MNRIQGWMALLIAAVLLAGLAGCGEAPEMPEGEPAAETAPASTP
                     SAVATPRFESGMIPPAIVIPTVTPDAAPAASPDAASATELPDAVTIAMIAKSSTNPVF
                     LSARTGAEAAARELSERHGVTITIDWRTPPTEDGQVQAQRIAQAVNEGADAILISSSD
                     AAKVTGAINDAVARGVP"
     gene            complement(656..1711)
                     /locus_tag="PLS90_17080"
     CDS             complement(656..1711)
                     /locus_tag="PLS90_17080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK04161.1"
                     /translation="MPPLPPRRLLQPLQHIVYVQQSGLLLVILALGALLAFLAGSHTD
                     RATGEAVNNFLNSYTLMQVATDASFFAIMAVGATMVIISGGIDLSVGSVYALAGVLTA
                     MALRHLTHLAPAAQVGCAMGLCLGLGLACGLANGLMIVGLRVHPFIITLGMMWVFRGI
                     AFVASKAQSILLPRPVTEAVKSTLGFGESLYPVPLIVMLAITAAGAIYLTRTVMGRHL
                     YAVGGNAEAARYAGLRLGPILVGVYAISGLTAGIAAFLGSGFYGSASCADATGYELYV
                     IAAAVVGGASLAGGRGSAVNAMLGALLIVLIRQSIRTLHFDQNYEWIIIGCAIVVAVV
                     LDQLNARLAARRLARGR"
     gene            complement(2077..>2703)
                     /locus_tag="PLS90_17085"
     CDS             complement(2077..>2703)
                     /locus_tag="PLS90_17085"
                     /inference="COORDINATES: protein motif:HMM:NF014456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPK04162.1"
                     /translation="LEERGHELIDTTCPLVRRAHAAACKLARMGCLVLVIGRRGHVEV
                     EGLTGDLEQCEVVQSAAEVRAYDAPRLGIVCQTTTAPAVANQIVDRIKELNPDREIHL
                     IATICRPTLDRQAAVRSLLGRVDALVVVGGRRSNNTRQLAGLAEAAGKPWLHVEGPDE
                     LQPEWFAAFETVGLTAGTSTPDDVIGAVHQRLRAIAAAHERDQLPEAM"
BASE COUNT          449 a          963 c          889 g          402 t
ORIGIN      
        1 cggcacgccg cgggcgacgg catcgttgat cgcgccggtc accttggcgg cgtcggagct
       61 ggagatcagg atcgcgtcgg ccccttcatt gaccgcctgc gcgatgcgct gggcctgcac
      121 ctgaccgtct tcggtgggcg gcgtgcgcca gtcgatcgtg atcgtcacgc cgtggcgctc
      181 ggagagctcg cgcgcggccg cctcggcccc cgtccgcgcc gagaggaaga ccggattggt
      241 cgagctcttg gcgatcatcg cgatcgtcac ggcgtcgggc agttcggtcg ccgacgccgc
      301 gtcgggcgac gcggccggcg cggcgtcggg cgtcacggtc ggtatgacga tcgcgggcgg
      361 gatcataccg gattcaaacc gcggcgtggc cacggccgac ggcgttgagg ccggcgccgt
      421 ctccgccgcc ggctccccct cgggcatctc cggcgcctcg ccgcagcccg ccaaccccgc
      481 cagcagcacc gccgcgatca gcagcgccat ccatccctgg atgcgattca tggttctcac
      541 tccttacgca agaatacgtc tctcgtcaga atacgtctct cgtctctcgt cctcgtaatc
      601 gcccccaaag gcccgcgcaa aattcagctc tcggctcgcg cccttcgtct tcggctcagc
      661 ggccccgcgc caaccggcgc gcggccagcc gcgcattgag ttgatccagc accaccgcca
      721 cgacgatagc acacccgatg ataatccact cgtagttctg gtcgaaatgc agcgtgcgga
      781 tcgactggcg gatcagcacg atcaagagcg cgccgagcat ggcgttgacg gcgctgccgc
      841 gcccccccgc gagcgacgcg ccccccacga ccgccgccgc gatgacatag agctcatagc
      901 cggtggcgtc ggcgcaggag gccgagccgt agaatccgct gccgagaaag gccgcgatac
      961 cggcggtcag gcccgagatg gcataaacgc cgaccaggat cggccccagc cgcaggccgg
     1021 cgtagcgcgc cgcctcggca ttgcccccca cggcgtagag gtggcgcccc atcaccgtgc
     1081 gcgtcaggta gatcgccccc gccgccgtaa tcgccagcat cacgatcagc ggaacggggt
     1141 agagcgattc gccgaacccc agcgtcgatt tgaccgcctc ggtgacgggc cgcggcaaca
     1201 ggatgctctg cgccttgctg gcgacgaacg caatcccgcg gaaaacccac atcatcccca
     1261 gcgtgatgat gaacgggtgg acgcgcagcc cgacgatcat cagcccgttg gcgaggccgc
     1321 acgccaggcc gaggccgagg cacaggccca tcgcgcagcc gacctgcgcg gccggcgcca
     1381 ggtgcgtcag gtgccgcaac gccatcgccg tcagcacgcc ggcgagcgca tagaccgacc
     1441 cgaccgacag atcgatgccg cccgaaatga tcaccatcgt cgcgccgacg gccatgatcg
     1501 caaagaacga cgcatcggtg gcgacctgca tcagcgtgta gctgttgagg aaattgttga
     1561 ccgcttcgcc ggtggcgcgg tcggtgtgcg agccggcgag gaacgcaagg agcgctccca
     1621 gcgccagaat gaccagcagc agccccgatt gctgcacgta gacgatgtgc tggagcggct
     1681 gaagcaggcg gcgtggcggc aaagggggca tgggcgcgat gggagccggg cacagccgcg
     1741 gcatggaatt caagcaaagc ggtcccgcga acgcgcccat tctaatcaaa ggggaggcga
     1801 atacaagacg cagtgaccgt ttgacggccc gacctggcgg ctggcccttg cccgcgagga
     1861 accgtggttt gatgctgaga tcgacgaccc gcggttcaag gggctgaatg aacggccgag
     1921 gcggccggcg aagttcgagc gttctcgggt cgatttcccc accgatctaa aatcgtccct
     1981 cgtccttcgt cctcgtcctg gtgctcgccg ccaacggcct acccccattt ttacccctcg
     2041 tagccaactc gcggttcatg aacacaccgc gatgaactac atggcctcgg gcagttggtc
     2101 cctttcgtgc gcggccgcga tggcgcgcag gcgctggtga acggcgccga tcacgtcgtc
     2161 gggcgtggag gtgccggccg tgaggcccac ggtttcaaag gcggcaaacc attccggctg
     2221 gagttcgtcg ggcccctcca cgtgcagcca cggcttgccc gccgcctcgg ccaggccggc
     2281 cagctggcgc gtgttgttcg accgccggcc gccgaccacg accagcgcgt cgacgcgccc
     2341 cagcaggctg cgcactgccg cctggcgatc gagcgtggga cgacagatcg tcgcgatcag
     2401 atggatctcg cggtccgggt tgagttcctt gatccggtcc acgatttgat tcgccacggc
     2461 cggcgcggtc gtcgtctggc agacgattcc cagccgcggc gcatcgtagg cgcgcacttc
     2521 cgccgccgac tgcacgactt cacactgctc cagatcgccg gtcagcccct ccacctcgac
     2581 atggccgcgc cggccgatca ccagcaccag gcaccccatg cgcgcgagct tgcaggcggc
     2641 cgcatgggcg cgccgcacga gcgggcaggt ggtgtcgatg agctcgtggc cgcgctcctc
     2701 cag
//
LOCUS       DAOPCO010000415         2684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_29963_length_2684_cov_4.393305, whole genome shotgun sequence.
ACCESSION   DAOPCO010000415 DAOPCO010000000
VERSION     DAOPCO010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29963_length_2684_cov_4.393305"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..432
                     /locus_tag="PLS90_17090"
     CDS             175..432
                     /locus_tag="PLS90_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPK04163.1"
                     /translation="MPPEGPQRPMRPRRLQGPLGQAGMAFLIPGLLLAGPVSGYLLGL
                     LIQRWTGWGQWVLLLMVALGLITGIRESIRIIRNLQRQSDS"
     gene            429..941
                     /locus_tag="PLS90_17095"
     CDS             429..941
                     /locus_tag="PLS90_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04164.1"
                     /translation="MTAQPLTARQPAMRRPSPPFTALRSAFLRRTAVAIGATACVVAA
                     LGAAGLGPRWGGQYFLAALWSTLFLALTPLIIKTMLFDRRMGLGVLLMGAKLGLMVVL
                     YLTCRAWVGGEAHRLQVMTAVLSGLVTPLLVVTLRAIGAIGGGESRESVLREIRGERR
                     PAAHQTENQA"
     gene            938..2053
                     /locus_tag="PLS90_17100"
     CDS             938..2053
                     /locus_tag="PLS90_17100"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPK04165.1"
                     /translation="MSPRTSMHLADGGAIMLAQLGTGEPHGEVTHGPGLHGDEQQFHG
                     AGEEQPLTGAHAAEAHAEAADGGEHHPPAQYLLPHEVPNIATLAEAVADPAHFEIHGS
                     HRPHPAAYVGWVPINFLFSICLAGCLVLFFRRAMRPRAVRRPGRLQLFAEMMLGGMRG
                     FIIDIMGEEQGRRHFPYLASLWFFILVNNLAVLVPGLKAPTASLKTTLALGLTSIVYV
                     RIATLRDIGPLGYVYHLAGSPKDVITWCMAPLFIVIELIGEVVKPVSLSLRLFGATLG
                     EDKVLAAFLGIGMMITAALLSDATPLVGLPLHVIFFPVVILLSTIQATVFAVLSAVYI
                     MMMQHHEEHEEGHGHEPAPATEGGRREAAAGAAGLAH"
     gene            2126..2383
                     /locus_tag="PLS90_17105"
     CDS             2126..2383
                     /locus_tag="PLS90_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPK04166.1"
                     /translation="MENFVPASLILFAYHLSVAVAAIGSGFGLGRAVGSAMEAMGRQP
                     EASGKIQLAMIIGCAFIEMLTIYALISIFFVGGVIGTGMEG"
     gene            2521..>2684
                     /locus_tag="PLS90_17110"
     CDS             2521..>2684
                     /locus_tag="PLS90_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04167.1"
                     /translation="MSYELWTQIGETITQIIAFLIFVWAMKKFVWPILAAVLDERQRK
                     IEEGFADIERR"
BASE COUNT          396 a          794 c         1019 g          475 t
ORIGIN      
        1 gggggagctt tgcggcatgc ggcggcggca tgggtcggct ccgcgcgtcc gcaaagggca
       61 gcagcgcgga ggtcgcagcg gaccgacggg ctgttttttt gcctgggacc gactggcaca
      121 agggactgag tttgtgatat ttttcgcgaa ctcggccgcg cgagggaagt cgccatgccc
      181 ccggaaggtc ctcagcgccc catgcggccc cggcggctcc agggtccgct cggccaggcc
      241 ggcatggcct tcctgatccc gggtctgctg ctggccggac cggtttcggg ctacctgctc
      301 gggctgctga tccagcgctg gaccgggtgg gggcagtggg tcctgctgct catggtggcc
      361 ctgggtttga tcacggggat tcgggaatcg atccggatca tccgtaacct gcaacgtcaa
      421 tcggattcat gaccgcacaa cccctcaccg cccggcagcc cgcgatgcgc aggccgagcc
      481 ctccctttac ggctttgcgc agcgcgtttc tgcgccgaac ggcggtcgcg atcggggcga
      541 cggcgtgcgt ggtggcggcg ctgggggccg ccgggctggg tccacgctgg ggcgggcagt
      601 attttctggc ggcgctgtgg tcgacgctct ttctggcgct gacgccgctg atcatcaaga
      661 cgatgctgtt cgaccggcgg atgggcctgg gcgttctgct gatgggggcc aagctggggc
      721 tgatggtcgt gctgtatctg acctgccggg cgtgggtcgg cggcgaggcc caccggctgc
      781 aggtaatgac ggcggtgctg agcgggctgg tgacgccgct gctggtggtg acgctgcgcg
      841 cgatcggggc gatcgggggc ggcgagtcgc gcgagagcgt gctgcgcgag attcggggcg
      901 aacggcgccc ggcagcccac cagacggaga atcaggcatg agtccacgga cgagcatgca
      961 cctggcggac ggcggcgcga tcatgctggc gcagcttggc acgggggagc cgcacggcga
     1021 ggtgacgcat gggccgggcc tccatggcga cgagcaacaa ttccatgggg cgggcgagga
     1081 gcagccgctg accggcgcgc acgcggccga ggcgcacgcg gaagccgccg atggcggcga
     1141 acatcatccg ccggcacagt atctgctgcc gcacgaggtg ccgaacatcg cgacgctggc
     1201 cgaggcggtg gcggatccgg cgcacttcga gattcacggg tcgcaccggc cgcatccggc
     1261 ggcgtatgtg ggctgggtgc cgatcaattt tctgttcagc atctgcctgg cggggtgtct
     1321 ggtgctgttt ttccggcggg cgatgcgacc gcgggcggtc cggcggccgg ggcggctgca
     1381 actgttcgcc gagatgatgc tgggcgggat gcgcggcttc atcatcgaca tcatggggga
     1441 ggagcagggc cgccgccact tcccgtatct ggcgtcgctg tggtttttca tcctggtcaa
     1501 caacctggcg gtgctggtgc cggggctgaa ggcgccgacg gcgagcctga agacgacgct
     1561 ggcgctcggg ttgacgtcga tcgtctacgt ccggatcgcg acgctgcgcg acatcggacc
     1621 gctggggtat gtctatcatc tggccggctc gccgaaggat gtgatcacgt ggtgcatggc
     1681 gccgctgttc atcgtgatcg agctgatcgg cgaggtggtc aagccggtgt cgctcagtct
     1741 gcgactgttc ggggcgacgc tgggggagga caaggtgctg gcggcgttcc tggggatcgg
     1801 gatgatgatc acggcggcgc tgctgagcga cgcgacgccg ctggtgggcc tgccgctgca
     1861 cgtcatcttc ttcccggtgg tcatcctgct gtcgacgatc caggcgacgg tgtttgcggt
     1921 cctgtcggcc gtctacatca tgatgatgca gcaccacgag gagcatgagg aggggcacgg
     1981 acacgagccg gcgccggcga cggaaggcgg acggcgcgag gcggccgcgg gtgcggcggg
     2041 cctggcgcac tgagccgaga ggcaacaagt ttgaaaaccc ctgacggggt ggacccaaca
     2101 cccgcttgct ggaggaaaga ggaccatgga gaacttcgtt cccgcatcgt tgatcctgtt
     2161 cgcatatcac ctgtcggtgg ccgtggcggc catcgggtcg ggcttcggcc tgggccgcgc
     2221 cgtcggttcg gcgatggagg cgatggggcg tcagcccgaa gcgtcgggca agatacagtt
     2281 ggcgatgatc atcggttgcg cgttcattga gatgctgacg atttacgcgt tgatctcgat
     2341 cttcttcgtg ggcggtgtga tcggcaccgg catggaaggc tgaggccgaa gatccggcaa
     2401 gcggttgaag cgccgcccgt tcgcgccgcc acggcgggcg ggcggccagc ctcttcgcca
     2461 tgatgagtgg cccttgcgcc gccgcgcggc ggcggtcaat gcgcaaagga agacgaccag
     2521 atgtcctacg aactgtggac tcaaatcggc gaaacgatca ctcagatcat cgccttcctg
     2581 atcttcgtgt gggcgatgaa gaagttcgtc tggccgatcc tggcggcggt gctcgacgag
     2641 cgccagcgca agatcgagga gggcttcgcc gacatcgagc gtcg
//
LOCUS       DAOPCO010000416         2671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_30145_length_2671_cov_4.939602, whole genome shotgun sequence.
ACCESSION   DAOPCO010000416 DAOPCO010000000
VERSION     DAOPCO010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30145_length_2671_cov_4.939602"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLS90_17115"
     CDS             complement(<1..402)
                     /locus_tag="PLS90_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPK04168.1"
                     /translation="MAERKRQVVIRAARRAMLCLRMDLDTLFTPYFNKWLERFGDGEL
                     QYVPRLLDQARELEVPLHFFAQGMGVIQYPDLLRRILAAGFAVDSHLHTHRITLIDDY
                     GLIFEEVLTAEHLLKSIGAAPTGIGATGMYPE"
     gene            complement(430..1284)
                     /locus_tag="PLS90_17120"
     CDS             complement(430..1284)
                     /locus_tag="PLS90_17120"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HPK04169.1"
                     /translation="MVTATQPRTVCELKQMTPEEQYFYDVNGYLVVENAIEPDLLRRI
                     LEWTAPLEAEARERIERMGPGENREYIFEDILNTETRLVELVANPTIVPYLNEMIECP
                     RLKSTWLTYKLKHGRTGAHSNHTPTRTCDYYHFNGRIYHNLFQVFYALDDIGPGEGAL
                     QVIPGSHKANFPLPENDAFVEKLCIEVHQPRGSALLFSHDCHHRSYNHSDKMRKTLIF
                     TYCPGVIANSYGTDALYERLFEESPEHSWQKYLLRSPNGYRETYPCPVDNPWVRQGAP
                     KNYSAGKK"
     gene            1916..>2671
                     /locus_tag="PLS90_17125"
     CDS             1916..>2671
                     /locus_tag="PLS90_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04170.1"
                     /translation="MSGLLKQACVLLLALAAVPAFAENQISWIDDNGTSITFVRVSDG
                     AVFTQDFAGVLPSYNVPYDWDGDGFDDVLHIVSKPSSNNGFGLEIWSPMANGMTGGVL
                     VETDDLNVNFSSAAVIYRGDLVRSTSLDDLAVVVPGGGGVWPRLCYLDGRPLDANGNV
                     VSDATAYQGYATGDRDGNGIDEIYYARGSGIWFREYDGTTNTGEQKFADHAGGDLGYL
                     QLIGDVDGDGTDDVVSLNNTNNHIVYAPIDATIP"
BASE COUNT          459 a          863 c          778 g          571 t
ORIGIN      
        1 ctccggatac atgccggtgg cgccgatccc cgtcggcgcg gcgccgatcg atttgagcag
       61 atgctcggct gtcagcacct cctcgaaaat caggccgtag tcgtcgatca gcgtgatccg
      121 gtgggtgtgc agatggctgt caacggcgaa cccggccgcg agaatccgcc gcagcaggtc
      181 ggggtattgg atcacgccca tgccctgcgc gaagaagtgg agcggaactt ccagctcgcg
      241 cgcctggtct agcagccgcg gcacgtactg cagctcgccg tcgccaaacc gctccagcca
      301 cttgttgaaa tagggcgtga acagcgtgtc gaggtccatg cgcaggcaaa gcatcgcccg
      361 ccgcgccgcg cgaatcacga cctggcgctt tcgttccgcc atcgtcccca ccccgccgcc
      421 gatccgggct tacttctttc ccgcggagta gtttttcggc gcgccctgcc gaacccacgg
      481 attatccacc gggcaggggt aggtctcgcg gtagccgttg gggctgcgca gcaggtactt
      541 ttgccaactg tgttccggcg actcctcgaa cagccgctcg tacagcgcgt cggttccgta
      601 ggagttggcg atcacgccgg ggcaataggt gaagatcagc gttttgcgca tcttgtcgga
      661 atggttgtag gaacggtggt gacaatcgtg cgagaagagc agcgctgagc cgcgcggctg
      721 atgcacctcg atgcacagct tctcgacgaa cgcgtcgttc tccggcagcg gaaagttggc
      781 tttgtggctt ccggggatca cttgcagcgc cccttctccc gggccgatgt cgtccagcgc
      841 gtagaatacc tggaacaggt tgtgatagat gcgcccgttg aaatgataat aatcgcacgt
      901 ccgcgtcggc gtgtgattgg aatgcgcccc cgtgcggcca tgcttcagct tgtaggtcag
      961 ccaggtcgat ttcagccggg ggcattcgat catctcgttc aggtagggaa cgattgtcgg
     1021 gttggccacc agctcgacca gccgcgtctc ggtgttcagg atgtcttcga aaatatattc
     1081 gcggttttcg ccgggcccca tccgttcgat ccgttcgcgg gcttccgctt ccagcggcgc
     1141 ggtccactcc aggatccggc gcagcaggtc cggctcgatc gcattctcaa cgaccagata
     1201 tccgttcacg tcgtaaaaat actgttcctc gggcgtcatc tgcttcaatt cgcagacggt
     1261 ccgcggctgc gttgctgtca ccatcttgag ctctcccgtg cggtagcggt atgggttgtc
     1321 gcttgcaggt tcccacttca gcatagtcgg ccgccggcgg catctggtca gttgaccttt
     1381 tgttttctcg gcttgtgctc gtctccaacc ttcctgtttg ccgtcgagtc tctgatctac
     1441 gtccctcgtc ctcatcctgg gctttcgacc gttgccggca tgcgtcggca tcttcgatcg
     1501 gcaggagtca tgaattccgg cgacccaaac cacaataaaa gaggtaacca gcttcaatta
     1561 tcgtgattca cgccacgctc ggcctcctct cgttcttctc ccggttcgcc tctccgcttt
     1621 cttcgtgctc ctgctgcctc tttcaagcca aaatcagcca agcattggac aagaggtggg
     1681 ttgtcccgcc ccggccgccg ggtctacgct gatttcagcg gttacattca gcagggtttg
     1741 agggccgtaa ccccccacgg acgggcggca cacgcaggac ccttggaccg acccagtcga
     1801 ccttgcggga ggattgccta tgcgaaggat gatctgacct ccgacccttt tgctgacatc
     1861 ccgtttccga tcatactgtc tttgcatcac ttatatttga ggggaggctc ttcacatgtc
     1921 cggattgtta aagcaagcct gtgttctcct gctcgcgctc gccgcggtgc cggcgttcgc
     1981 cgagaaccag atcagttgga tcgacgacaa tggcacaagc attaccttcg tgcgcgtctc
     2041 cgacggcgcc gtcttcaccc aggatttcgc gggtgtgctg ccctcctaca acgttccgta
     2101 cgactgggat ggcgacggct tcgatgacgt ccttcacatc gtcagcaagc ccagcagcaa
     2161 caacgggttc ggccttgaga tctggagccc gatggccaac ggcatgaccg gcggcgtgct
     2221 ggtcgagacc gacgacctga acgtcaattt ctcctccgcc gccgtgatct atcggggcga
     2281 cctggtccgc tccacgtcgc tcgacgacct ggcggtggtc gtccccggcg gcggcggcgt
     2341 ctggccccgc ctgtgctacc tcgacgggcg cccgctcgac gccaacggca acgtcgtctc
     2401 cgacgccacg gcctatcaag gttacgccac cggcgaccgc gacggcaacg ggatcgatga
     2461 gatctactat gcccgcggca gcggcatctg gttccgtgaa tacgacggca cgaccaacac
     2521 cggcgagcag aaattcgccg accatgccgg cggcgacctg ggctatctcc aattgatcgg
     2581 cgatgtcgac ggcgacggca ccgacgatgt ggtctcgctc aacaacacca acaaccacat
     2641 cgtctatgcc ccgatcgacg cgacgatccc c
//
LOCUS       DAOPCO010000417         2641 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_30577_length_2641_cov_4.804331, whole genome shotgun sequence.
ACCESSION   DAOPCO010000417 DAOPCO010000000
VERSION     DAOPCO010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30577_length_2641_cov_4.804331"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLS90_17130"
     CDS             complement(<1..484)
                     /locus_tag="PLS90_17130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPK04171.1"
                     /translation="MSQEDSPPAAPAAPRFSVIVPVRNRPADIRRCVEAVHALDFPRN
                     AYELIVVENGSTDATAREARAAGARVIHEPIVNRCRARNLGALAARAPWLVFLDSDCV
                     PDPDWLAAFDAFLENLSPADRERTAIVAGAVLDVPPANVVEEYIARRGWFDQEKYLRQ
                     Q"
     gene            998..1459
                     /locus_tag="PLS90_17135"
     CDS             998..1459
                     /locus_tag="PLS90_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04172.1"
                     /translation="MANERPTRLMIDGWCSLAVPTGWAWNQEEGITSVYREAEPQGAL
                     NISLYFRRGQRQPPSRPELVEYARKLARALGNPVETNAVGIEPVGQGEGSYVQLRDNL
                     GDRWRIWHTGAVGRVVCLAYQQPVESSDQEHEDVERMVRSFQWLEEKPVDL"
     gene            1521..>2641
                     /locus_tag="PLS90_17140"
     CDS             1521..>2641
                     /locus_tag="PLS90_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04173.1"
                     /translation="MLSEGPAATIAPRRARPALLLLCALYLLIFGRYLIGFAPAGGDI
                     VNQYLPYQQLVREAIRGGDAPLWNAMTFCGRPLMADIQVGVLYPPNWLHWLLPLPLGF
                     GLLLALHWGWMLWGCWKLGGHWRLAPEATALGALLFALSPFFTLKLSSGIILFLYVGS
                     WWPWLALGVSRLVERPSFARLAQLTLVLALSLLAGSPQITFYGWIMAAAFALGLGIAG
                     AGGGGGSRRRRAAAVARGLGWFTLAVALALGLTALQTAQTFHFIRQSFERGAGGAGWD
                     YVTDGSLSPGILWLLLNPGYLGIGHSELALYFGSRLDFSEACFYSPLWVSLLLIPAGA
                     PCILQLIRTESIEILRRRRLAALAVLAVVAVLLGLALALG"
BASE COUNT          396 a          788 c          999 g          458 t
ORIGIN      
        1 cctgctggcg caggtatttc tcctggtcga accagccgcg ccgcgcgatg tattcctcga
       61 cgacgttggc gggcgggaca tcgaggacgg cgccggcgac gatggcggtc cgttcacggt
      121 cggccggcga caaattctcc aggaaggcat caaacgcggc gagccagtcc ggatcgggga
      181 cgcagtcgct gtcgaggaag acgagccacg gggcgcgggc ggccagggcg cccaggttgc
      241 gcgcgcggca gcggttgacg atcggttcat ggatcacgcg ggcgccggcc gcgcgcgctt
      301 cgcgcgccgt ggcgtcggtg gagccatttt cgaccacgat caattcatag gcattgcggg
      361 ggaagtcgag ggcgtgaacg gcttcgacgc agcggcggat gtcggccggc cgattgcgca
      421 ccggaacaat gacggagaag cgcggagcgg ccggggcggc gggaggggaa tcttcttggc
      481 tcaacgacgg gagaaccttt agagtggggc gcgaacggga tggccatgtt cggatcgctc
      541 cagggtggag gttactgtag ctttcgaggg ggtggttggg aagaaaattg agggggatcg
      601 atcgtatcga atagctcatg accggggggg cggaaccagg gacaaaacgg ccggttttcg
      661 agagccgagg acgagggacg agcgccggga gccggatttt cgggcaggcc ttcgggggcg
      721 attacgagtt gctcatgaac cggcggttca caacgaggga tgaaaatggg gagggaacga
      781 ccgattcgac cgcttcaagc catgccggat cctcccgcag agcagtcccg tcagggacgg
      841 cagacttcag cccgccacgt cagtggcggg aaccgggatc cccagagcga ccgagtccca
      901 tagggacggc agaaccaatc caagtaaccc gagaataggg gacaaatttt cgagtaagga
      961 cgaggggcga gggacggatc tttgcgtggg ggacgagatg gcgaacgagc gaccgacacg
     1021 gctgatgatc gacgggtggt gctcgctggc ggtgccgacc ggctgggcgt ggaaccagga
     1081 ggaagggatc accagcgtct accgcgaggc ggagccgcag ggcgcgctca acatctcgct
     1141 ctattttcgg cgcggccagc gccagccgcc ctcgcggccc gaactggtcg aatacgcccg
     1201 caagctggcg cgggcgctgg gcaacccggt cgagacgaac gcggtcggca ttgagccggt
     1261 ggggcagggc gaggggtcct acgtccagtt gcgggacaac ctcggcgacc gctggcgcat
     1321 ctggcatacc ggcgccgtcg gccgtgtggt ctgtctggct tatcagcagc cggtcgaatc
     1381 cagcgaccag gaacatgagg acgtggaacg catggtccgc tcatttcagt ggctcgaaga
     1441 aaagccggtc gacctgtagc gatgctcggc gcgtgggtca ttggctcgat ggaaaccggc
     1501 gctgacggcg ggtcgcgcgc atgctgagcg agggtccggc agcgacgatt gccccccgcc
     1561 gcgcccggcc ggcgctgctg ctgctctgcg cgctttacct gctgatcttc ggccgctatc
     1621 tgatcggctt cgccccggcc gggggcgata tcgtcaacca gtatctgccc taccagcagt
     1681 tggtccgcga ggcgattcgc ggcggcgatg cgccgctgtg gaacgcgatg acgttctgcg
     1741 ggcggccgct gatggccgac attcaggtcg gcgtgctcta cccgcccaac tggctgcact
     1801 ggctgctgcc gttgccgctc ggcttcggcc tgctgctggc gctgcactgg gggtggatgc
     1861 tgtggggctg ctggaaactg ggggggcatt ggcggctcgc gccggaggcg acggcgctcg
     1921 gcgcgctgct gttcgcgctc tcgccgttct tcacgctgaa actcagctcg ggcatcatcc
     1981 tgtttctcta tgtcgggtca tggtggccct ggctggcgct gggcgtcagc cgactggtgg
     2041 agcggccgtc gtttgcgcgg ctggcgcagt tgacgctggt gctggcgctg tcgctgctgg
     2101 ccggatcgcc gcagatcacg ttttacggct ggatcatggc ggcggcgttc gcgctcggcc
     2161 tggggatcgc gggcgcgggg ggcggcggcg ggtcgcggag gcggcgcgcc gcggcggtcg
     2221 cgcgcggact gggctggttc acgctggcgg tcgcgctggc gctcggcctg acggcgctgc
     2281 agacggcgca gacctttcac ttcatccgcc agtccttcga acggggcgcg gggggcgccg
     2341 gctgggatta cgtcaccgac ggttcgttgt cgcccggaat cctgtggttg ctgctcaacc
     2401 ccggatatct gggcatcggc cattccgaac tggcgctcta cttcgggtcg cggctcgatt
     2461 tctccgaagc ctgcttctat tcgccgctct gggtgtcgct gctcctgatt ccggcgggcg
     2521 cgccgtgcat cctccagctg attcgaacgg agtcgattga gattctccgg cgccggcggc
     2581 tcgcggcgct ggcggtgctg gccgtggtgg ccgtcctgct cgggctggcg ctggcgctcg
     2641 g
//
LOCUS       DAOPCO010000418         2626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_30797_length_2626_cov_5.296772, whole genome shotgun sequence.
ACCESSION   DAOPCO010000418 DAOPCO010000000
VERSION     DAOPCO010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30797_length_2626_cov_5.296772"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLS90_17145"
     CDS             complement(<1..775)
                     /locus_tag="PLS90_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HPK04174.1"
                     /translation="MPRYRLAFGLVLLTLVLTYCLIVLGGIVHNTGSSLACPDWPKCF
                     GQWMPEMTGGVFYEHSHRMLGTLVGLCAIALCIVLWRPAPDFPSIRHHGLILLGIIII
                     QGILGGITVLYQLPDLVSTAHLGTSMLVMLYMIYLANETFRRGRRTGGQAAATTYDAA
                     LAAPYLRLIVIILAVLYAQIVFGAFIRHSGASAAAGQGMRNALLGSDPNTGQIAVWPS
                     NGCALLNVLHRYVGVVLGVAVVALSVKLNTGLGRQSSPHR"
     gene            complement(869..1486)
                     /locus_tag="PLS90_17150"
     CDS             complement(869..1486)
                     /locus_tag="PLS90_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04175.1"
                     /translation="MLTFREFVTSEPLPLATVQDGVFEFLRERSDFVLSGAQAVNAYV
                     DEPRMSQDIDILSARAAELAEEIRIDLANRFKIAVRVRTVANQMGFRLYQVRSTGNRH
                     LVNVRRMASLPPSRIVDGIPVAIAEEVIARKVLACHRREGQPKADTDRRDLKMLLFKF
                     PELKMADGAVRDRLADLGAEPEAVDLWHRLAAEEIVEPHEDDEFF"
     gene            complement(1599..>2626)
                     /locus_tag="PLS90_17155"
     CDS             complement(1599..>2626)
                     /locus_tag="PLS90_17155"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPK04176.1"
                     /translation="QLVIQEHKEERHPQIQLLSPEGKKKDLIATYNLTSGAIIVSGIQ
                     DGAQVKAGQILARLPRLRLKSKDITGGLPRIDELFEARKPKDAAIIAEIDGEVQIRGI
                     QKGARRVAVVGADGEETLYSVPLIRHLIVRDGEKVKAGDPLTDGSVNPHDLLRIKGEK
                     EVQEFLLGEVQEVYRLQGVTINDKHIESIVRQMLRKVVVEDVGDTRLLYGQMLDKFEF
                     QEENERTIAEGGKPAVARPKLLGLTKASLETDSFISAASFQETTRVLTDAAVRGRYDY
                     LRGLKENVIMGLLIPAGTGLNVYKQYDLVPDMSKQEVVEILTAKEPEEEEEGDLVGAE
                     DGGDGRV"
BASE COUNT          472 a          901 c          778 g          475 t
ORIGIN      
        1 cccggtgcgg cgacgattgc cggcccagac ccgtgttcaa cttgaccgac agcgcgacga
       61 cggcgacgcc cagcacaacc ccgacgtagc ggtgcagcac gttgagcagc gcacagccgt
      121 tcgacggcca gacggcgatc tgccccgtgt tcgggtcact ccccagcagc gcgttgcgca
      181 tcccctgccc ggcggcggcc gacgcgccgg aatgccggat gaacgcgccg aagacgatct
      241 gcgcgtagag cacggcgaga atgatcacga tcagccgcag atacggcgcg gccagcgcgg
      301 catcatacgt cgtcgcggcc gcctgcccgc cggtccgccg gccgcgccga aacgtctcgt
      361 tggccaggta gatcatgtag agcatgacga gcatcgatgt gccgaggtgc gcggtggaga
      421 caagatccgg aagctggtag agcaccgtga tcccgccgag gatcccctga atgataatga
      481 tgccgagcag aatcaatccg tgatgccgga tcgacggaaa atccggcgcc ggccgccaga
      541 gcacgatgca cagcgcgatc gcgcacagcc cgaccagcgt ccccagcatc cggtggctgt
      601 gctcgtaaaa cacgccgccg gtcatctcgg gcatccattg gccgaagcat ttgggccagt
      661 ccgggcaggc caggctggaa ccggtgttgt gcacgatgcc gcccagaacg atcaggcagt
      721 aggtgagaac cagagtcagc agtacaaggc cgaaggccag acgatagcgg ggcatgagac
      781 gatcctctcg cgatgcttgc gaagcaccag cttcaatcgt ccccatcgta gcaaaaatgg
      841 tccgtcggat cagcagcgac gggaggagtt agaagaactc atcgtcttca tgcggttcga
      901 cgatttcctc ggcggcgagc cgatgccaca ggtcgacggc ctccggctcc gcgccgagat
      961 cggcgagccg atcgcgtacg gcgccgtccg ccattttcag ttccggaaat ttgaacagca
     1021 gcatcttcag atcgcgccgg tcggtgtcgg ccttcggttg gccttcgcgc cgatggcagg
     1081 caagcacctt gcgcgcgatc acttcctcgg caatggccac cgggatcccg tccacgatgc
     1141 gactgggcgg cagggaggcc atccgccgca cgttgacgag atggcgattc cccgtgctcc
     1201 gaacctgata gagccggaat cccatctggt ttgccacggt tcgtacccga acggcaatct
     1261 tgaatcggtt ggcgaggtcg atccgaatct cctcggccaa ttccgcggcc cgtgcggaga
     1321 gaatatcaat atcctggctc atccgcggct catcgacata ggcattcacc gcctgagccc
     1381 cagacaggac gaaatcggag cgctcgcgca gaaactcgaa gaccccatcc tgaacggtcg
     1441 ccagcggcaa gggttcagac gtgacaaact ccctgaatgt gagcacgcca gccggaatca
     1501 tgatcctctc cgtatgtgaa cgagcttgat tatatgcaaa acgggcccga gtggtctact
     1561 cgggcccgca tgattcgctt tcgattccgg cgtctggctc aaacccggcc gtcgccgccg
     1621 tcctccgcgc cgaccagatc cccctcctcc tcttcttccg gttcctttgc cgtcaggatc
     1681 tcaaccactt cctgcttcga catgtcgggc accagatcat actgcttgta gacgttcagc
     1741 cccgtccccg ctggaatcag caggcccatg atcacgttct ccttcaggcc gcgcaggtaa
     1801 tcataccgtc cgcgcaccgc cgcgtcggtc agcacgcgcg tcgtctcctg gaacgaggcg
     1861 gccgagatga acgagtcggt ctcgagcgac gccttggtca gtcccagcag cttcggccgc
     1921 gcgacggccg gcttgccgcc ctcggcgatc gtccgctcgt tctcttcctg gaactcgaac
     1981 ttgtccagca tctggccgta gagcaggcgc gtgtcgccga cgtcctcgac gacgaccttg
     2041 cgcagcatct gccgcacgat cgactcgatg tgcttgtcgt tgatcgtcac gccctgcagg
     2101 cggtagacct cctgcacctc gcccagcagg aattcctgca cttccttctc gcctttgatc
     2161 cgcagcagat cgtgcgggtt gaccgacccg tcggtcagcg gatcgccggc cttcaccttc
     2221 tcgccgtcgc ggacgatcag gtgccggatc agcggcaccg agtagagcgt ctcttccccg
     2281 tcggcgccga cgaccgccac ccgacgggcg cccttctgga tcccgcggat ctgcacctcg
     2341 ccgtcgatct ccgcgatgat cgccgcgtcc ttcggcttgc gcgcctcgaa cagctcgtcg
     2401 atccgcggca gaccgcccgt gatgtccttg ctcttgagcc gcagccgcgg caggcgtgcc
     2461 agaatctggc ccgccttgac ctgcgctccg tcctgaatcc ccgacacgat gatcgcgccc
     2521 gacgtcaggt tgtacgtcgc gatcagatcc tttttcttcc cttcgggcga aagcagctgg
     2581 atctgcgggt gccgttcctc cttgtgctcc tggatcacga gctgca
//
LOCUS       DAOPCO010000419         2601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_31176_length_2601_cov_4.743126, whole genome shotgun sequence.
ACCESSION   DAOPCO010000419 DAOPCO010000000
VERSION     DAOPCO010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31176_length_2601_cov_4.743126"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLS90_17160"
     CDS             <1..937
                     /locus_tag="PLS90_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04177.1"
                     /translation="MVAAPRGWRLNWWGVWLWLLAANVGFYAVHADLITPGMRAALET
                     AEELDRSMPRGHQLIVLRDGIKEKHAVAEQISFRVPGREIYIDESLQDWFAEPVKVDE
                     NLTLETGLPADSTWVLTAGEWRFNRPPRGRVGSLRRYSMADGPLMFDRAQYERYLEDP
                     QTLKENDFRKVLKDLQFSVVSASVPAVLAAGEETPLRFRIRNDGFFATPEPGEVRYEI
                     GYHWSAPNITGDWTAIVWDDGNSAAIPPGVARGQDFELTIPVRAPEEPHGLLHLELRV
                     LGVKDDLREFGQDSVDLRQILVYPEGRMPDSTPTE"
     gene            1014..2297
                     /locus_tag="PLS90_17165"
     CDS             1014..2297
                     /locus_tag="PLS90_17165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HPK04178.1"
                     /translation="MPPKAKAVIMTVGLSTEPGRDVLDVLELDLRELEPEYLAVIASD
                     ASRANGRRLIERSGLAADRAEIVEVSDARDLDDIFRKTNELIQRLVEQGYAPDEIAVN
                     YTAGTKVMGSGAVLSAVYNKVMELRYITGLTAPGDRPQPVRHRIVTTRPGAVFAYQDL
                     LTGRNMLLDLRYRSALDVLAGIDEEFLSPSDRQQLAALIKLTRAYDAWDNFQYGQFLL
                     RYEEITFNGENLRAFRLEATQHELVRRVAHETAARNIGPHIIAEMFNNAERRLMVGRI
                     EDAMARLYRCLELLAQWVLLREYGIDTNDVDTRRVPPRDRVALEALRSLEDGMVKIGL
                     RKSFDLLRILGARVGQVLEQDPVLRQFLELRSGSILAHGLQPVARGDGERFMTHAREV
                     FRIEIEQFDELTRQMQFPWLRDRPREAGPDQVRGE"
BASE COUNT          445 a          697 c         1030 g          429 t
ORIGIN      
        1 gatggtcgcg gcgccgcggg ggtggcggct gaactggtgg ggcgtctggc tctggctgct
       61 cgcggcgaac gtcgggtttt acgcggtcca tgccgatctg atcacgccgg ggatgcgcgc
      121 ggcgctggag acggccgaag aactggaccg gtcgatgccg cgcggtcatc agttgatcgt
      181 gctgcgcgac gggatcaagg aaaagcacgc cgtcgccgag cagatcagct tccgcgttcc
      241 ggggcgggag atctacatcg acgaaagttt gcaggactgg tttgccgaac cggtgaaagt
      301 ggatgagaac ctcacgctgg agacggggct gccggcggac agcacgtggg tgctgacggc
      361 gggggagtgg cgattcaacc ggccgccgcg ggggcgggtc ggctcgctgc ggcgctactc
      421 gatggccgat gggccgttga tgttcgatcg cgcgcagtat gagcgatacc tggaggaccc
      481 gcagacgctg aaggagaacg acttccgcaa ggtgctgaag gatctgcaat tcagcgtggt
      541 ttcggcgagc gtgccggcgg tgctggcggc gggggaggag acgccgctgc ggttccggat
      601 ccgcaacgac gggtttttcg ccacgcccga gcccggggaa gtgcgctatg agatcgggta
      661 tcattggagc gcgccgaaca tcacggggga ctggacggcg atcgtctggg atgatgggaa
      721 ttccgcggcc atcccgcccg gagtggcgcg gggtcaggat tttgagttga cgatcccggt
      781 gcgggcgccg gaggagcctc acgggctgct gcacctggaa ctgcgcgtgc tgggcgtcaa
      841 ggatgacctg cgcgaatttg gccaggacag cgtggatctg cggcagatcc tggtgtatcc
      901 ggaggggcgg atgccggatt cgacgccgac ggagtgaccg gttattcaag cgaacgcggc
      961 cggggcgaat ctggccgtcg cgttcatacc ctgcacaagc ggaacccttg cccatgccac
     1021 ccaaagccaa ggcggtcatc atgacggtcg ggttgagtac ggaacccggc cgcgacgtgc
     1081 tggacgtgct ggaactcgat ctgcgggaac tggagccgga gtatctggcg gtgatcgcgt
     1141 cggacgcgag ccgggcgaac gggaggcggt tgatcgagcg gtcggggctg gcggcggatc
     1201 gcgcggagat cgtggaagtg agcgacgcgc gcgacctgga cgacatcttc cgcaagacga
     1261 acgagctgat ccagcggctg gtggagcagg gctacgcgcc cgacgagatc gcggtcaatt
     1321 acacggcggg gaccaaggtg atgggttcgg gggcggtgtt gtcggccgtg tacaacaagg
     1381 tgatggaact acgctacatc acggggctga cggcgccggg cgaccggccg cagccggtgc
     1441 gccaccggat cgtgacgacg cggccggggg cggttttcgc ctatcaggac ctgctgaccg
     1501 ggcgcaacat gctgctggac ctgcgctacc ggtcggcgct ggacgtgctg gcggggatcg
     1561 acgaggagtt tctatcgccg tcggaccggc agcaactggc ggcgctgatc aagctgacgc
     1621 gggcgtacga cgcgtgggac aattttcagt acgggcagtt tctgctgcgc tatgaggaga
     1681 tcacgttcaa cggggagaat ctgcgggcgt tccggctgga ggcgacgcag cacgaactgg
     1741 tgcggcgggt ggcgcacgag acggcggcgc gcaacatcgg gccgcatatc atcgccgaga
     1801 tgttcaacaa tgcggagcgg cggctgatgg tgggccggat cgaggacgcg atggcgcggc
     1861 tgtaccgctg cctggagctg ctcgcgcaat gggtgctgct gcgcgagtac gggatcgaca
     1921 ccaatgacgt cgacacgcgg cgggtgccgc cgcgcgaccg ggtcgcgctg gaggcgctgc
     1981 ggtcgctgga ggacgggatg gtgaagatcg ggctgcgcaa gtcgttcgat ctgctgcgga
     2041 tcctgggggc gcgggtcggt caggtgctcg agcaggatcc ggtgctgcgt cagtttctgg
     2101 aactgcgctc ggggtcgatc ctggcgcatg ggttgcagcc ggtagcgcgc ggggacgggg
     2161 aacggttcat gacgcacgcg cgcgaggttt ttcggatcga gatcgagcag ttcgatgagt
     2221 tgacgcgcca gatgcagttc ccgtggttgc gcgatcggcc gcgcgaggcg ggaccggatc
     2281 aggtgagagg ggaatgaggg gggaacgacg gggctctttg gaggacttca ggagattttg
     2341 agagccgagg acgagggccg agagccgaga gccgagtttt tggggggccg ttgggggcga
     2401 ttacgagggc gagggacgag ggatagcgat aaggatatcg attccggtta agcggcgggg
     2461 cggggcgacc gataagcagg atgggagcat gccgcccacg gaccctgcct ggagcacgcc
     2521 gcatgagcac cgcctcatcg tttctggaac agccagccct ggcgctggag ctggatattc
     2581 tgcgccaact cgtggtgctg t
//
LOCUS       DAOPCO010000420         2553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_31954_length_2553_cov_4.582866, whole genome shotgun sequence.
ACCESSION   DAOPCO010000420 DAOPCO010000000
VERSION     DAOPCO010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31954_length_2553_cov_4.582866"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..687
                     /locus_tag="PLS90_17170"
     CDS             118..687
                     /locus_tag="PLS90_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04179.1"
                     /translation="MMSAKTQVLSVDLPQVRLLLDSRDAQGVERTLTALAGALNWHHP
                     FSGVAEAARQVLLDGEPTVPQAGLIYYGLVLQCDHQPLGGEWLAEFYERAARQCPKLK
                     VEAAHCELLKGIIFGRTPHLLPMRSQEQITSCFMTDEEGLDLAAALARNNDLQQEADE
                     DVYETIYDHLIPTLSHTIPEGHGFYFLTM"
     gene            complement(719..1447)
                     /gene="lipB"
                     /locus_tag="PLS90_17175"
     CDS             complement(719..1447)
                     /gene="lipB"
                     /locus_tag="PLS90_17175"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HPK04180.1"
                     /translation="MPSPDATDAATPSPPHRPQPERVLADAARAAFIPWGLRDYGDVL
                     ARQHALHARRVADRTPDTWLVGEHPTVVTQGVRGSKADLLTAARYPVFRINRGGQTTI
                     HNPGQLVIYPIVKTQGGLLAQAWLSRLLLAAVSDWIANLTGVATQLEKGKPGLFVDGR
                     KVAAIGIAIHRRVAMHGIAINVCNDLAPWQSIIPCGEPERRPITLSEAAGRPLDPADL
                     VAAIPAWLHSAWGYRRVDTLADAD"
     gene            1873..>2553
                     /locus_tag="PLS90_17180"
     CDS             1873..>2553
                     /locus_tag="PLS90_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04181.1"
                     /translation="MPSALPHSPTDGCDPARPRRERLVALLLLLLLVLVFWYPSGAYP
                     LQALAADAGLLLLTVGAAAAAFRQARGRLLASLGTPPLVLGLAFALWGALRWSFLLVP
                     APGRAWVVGLLWLGVSLACGAALIAWSGPLGNLLCYLRRGLAVAGLVFAAYAFYQYFI
                     LYPASLAVLRGELRGPVADLMTQSQLHALELRRIGGRLGDPNLFACWLAVLAVFGAGC
                     LAPGERAAW"
BASE COUNT          348 a          736 c         1030 g          439 t
ORIGIN      
        1 gacgagggac gggagacgag agacgagttt tggggtagac cgttgggggc gagggcgaga
       61 gacgggagac gaagttttga tgggagaaag acgaccagat gcccgacagg tccatggatg
      121 atgagcgcga agacgcaggt attatcggtt gacctcccgc aggttcgcct gctgctggat
      181 tcccgcgacg cgcaaggggt cgagcgcacg ctgacggcgc tggccggcgc gctcaactgg
      241 catcacccgt tttcgggcgt ggcggaggcc gcgcgccagg tcctgctgga cggcgagccg
      301 accgtaccac aggcggggct gatctattac ggtctcgtgt tgcagtgcga ccaccagccg
      361 ctgggcggcg aatggctggc ggagttttat gagcgggccg cgcgccagtg ccccaagctg
      421 aaggtggagg cggcgcactg cgaactgctc aagggcatca tcttcgggcg gacgccgcac
      481 ctgctgccga tgcggagcca ggagcagatc acgtcgtgct tcatgaccga cgaagagggg
      541 ctggatctgg cggcggcgct ggcgcgcaac aacgacttgc agcaggaagc cgacgaagac
      601 gtctacgaaa cgatctatga ccacctgatc ccgacgctga gccatacgat ccccgaaggg
      661 catggatttt attttctgac gatgtagggg cgggccaggg ggcgcgctcg gggccgggtc
      721 agtcggcgtc ggcaagggtg tcgacgcggc gatagcccca ggccgagtgg agccaggccg
      781 ggatggcggc gacgaggtcg gccggatcaa gcgggcggcc cgccgcctcg ctcagggtga
      841 tcggccggcg ctcgggctcg ccgcagggga tgatggattg ccagggggcg aggtcgttgc
      901 agacgttaat ggcgatcccg tgcatcgcga cccggcggtg gatggcaatg ccgatggcgg
      961 ccaccttgcg gccgtcgacg aacaggccag gttttccctt ttcgagttgg gtcgccacgc
     1021 cggtcaggtt cgcgatccag tcgctgacgg cggcgagcaa caggcgcgag agccatgcct
     1081 gcgccagcag gcccccctgc gtcttgacga tcgggtagat cacgagctgg ccggggttgt
     1141 ggatcgtcgt ctggccgccg cggttgatcc ggaaaaccgg gtagcgggcg gcggtgagca
     1201 ggtcggcttt cgagccgcgc acgccctggg ttacgacggt gggatgttcg ccgaccagcc
     1261 aggtgtccgg ggtccggtcg gcgacccggc gggcgtggag cgcgtgctgg cgcgccagga
     1321 cgtcgccgta gtcgcgcagg ccccagggga tgaatgcggc gcgggcggca tcggccagga
     1381 cgcgctcggg ttggggccgg tgcggcggcg agggggtggc ggcgtcggtg gcgtcgggcg
     1441 aaggcatggg tcagggttct cgcccgatga tcccacaggg gagcggccgg catcccgcaa
     1501 aaagtgcgga gtgtggagtg aggagttagg ggtgattgag cggtggttga gcgaagcaat
     1561 ggtagcggta cgtgggaggg ggctgacacg ggggcccatg gaaagccggc tcacgagggc
     1621 ttcgcggagg gcgacgcagg gttccggccc gcggccgtca ccctgcgctt gattccggcc
     1681 gatcgttgcg cggacttggc attcgccccc ttcgggaacg gggatttttc gcgtcggtgt
     1741 ccccaggttc cgctgcgctc caccaccggc taccatctgg catccctgcg ggatgcgggg
     1801 gaatgaccgg cgagggggcg gttccacacc agtggacaat cggggcgggg ggcggcagaa
     1861 tccgtttccg cgatgccgag cgctcttccg cactctccga ccgatggatg tgacccggcg
     1921 cggccccgcc gcgaacggct cgtcgcgctg ctgctgcttc tgctgctggt gctggtgttc
     1981 tggtatcctt cgggggccta tccgttgcag gcgctggccg ccgacgcggg gttgctgctc
     2041 ctgacggtgg gcgccgcggc ggcggcgttc cgtcaggcgc ggggacgcct gctggcatcg
     2101 ctggggacgc cgccgctggt gctgggcctg gcgttcgcgc tgtggggggc attgcgctgg
     2161 agtttcctgc tggtcccggc gccggggcga gcctgggtgg tggggctttt gtggctgggg
     2221 gtgagtttgg cgtgcggcgc ggcgctgatc gcgtggagcg gtccgctggg gaatctgttg
     2281 tgttatctgc ggcggggtct ggccgtcgcg gggctcgttt tcgcggcgta tgcgttctac
     2341 cagtatttca tcctctatcc ggcgtcgctg gcggtgctgc ggggggagct gcgcggcccg
     2401 gtggcggatc tgatgacgca gagccagctt catgcgctgg agctgcggcg aatcggcggc
     2461 cggctcggcg atccgaatct gttcgcctgc tggctggcgg tgctggcggt gttcggcgcg
     2521 ggctgtctgg cgccggggga gcgggcggcc tgg
//
LOCUS       DAOPCO010000421         2539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_32183_length_2539_cov_5.251208, whole genome shotgun sequence.
ACCESSION   DAOPCO010000421 DAOPCO010000000
VERSION     DAOPCO010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32183_length_2539_cov_5.251208"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..2355
                     /locus_tag="PLS90_17185"
     CDS             412..2355
                     /locus_tag="PLS90_17185"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HPK04182.1"
                     /translation="MADLSIASSLFEFAPEIFARGLQAGCAVACDVAGARAQFEPLEP
                     PRQAPGELLFQWVERASGLHAAIRVRREPRGAAIIQTTLSNPAAAASPPLTRLRPLLL
                     EWPHGMAGRVRLRSLSGGVCSAVYPPEAYRVRTVERRYGGGPEAFRLESHPTGGSSNR
                     DLPFLLLSCDEAGLVLAMEWSGRWVIEAGWGWEYERLHLQAEIPVHQLVLAPGESLEL
                     PAVHLVFTDADGLAGCTNAIRKHIRDCIIPRRAHTHVPAPITYDSWFGVQLDFDARFL
                     RDQMQVAGEVGLESFTIDAGWATSSQPDGSDWGGGNYERIDSGKFPNGIVADAAHPAG
                     ELGLALGLWFDPEQAPPASDLGRKHPEWFLGDSHHLDLTIPAAAQYLEDLIAGWIERA
                     DLGWVKWDYNIVFDQFTIFERHDPTGKYAFHYFKALYGIWDRLLARYPHVIIEQCAGG
                     GRRLDLATLRRGHVQWISDFTWDSDICRAMQANLNLILPGCLCATALHTVKGAAEPGL
                     SELDFVARMMGAVQFMGDLSVWPADLRRRARRLVELFKGYRHLLDQNYYQLTPPPAMP
                     EDGQAVQFVSETGEEWIVFVFANRQTGAGPLRLRGLRPAATYRIEEPLSERPWGDVSG
                     SELMEHGLRVDAAAPFTLLRGQA"
BASE COUNT          385 a          866 c          822 g          466 t
ORIGIN      
        1 ccggcctgcg gccgtcaccc tgcgctttat tccggtcgtc cgctgcgcgg acttgatcca
       61 agagacaagt cggaggcgcc cgcattggat ttggagtcct cgagcggatt gcatattggg
      121 gaatgacatc tactgaagta gcggactgaa gtcggccgtt tctgacggga ctgcgccact
      181 ggacgatccg gcaagccccc ggccgttcga atcggtgatt catggtccgc cattttcagc
      241 ccgtgctgtg aaccgcggga ttacgaggaa ctggagatcg tccacttcca cgtaaccggc
      301 atccgcgccc tcgcccacaa ccgcatccgc ttcgcattgg cattcctcgc tgagctgtgt
      361 catgttcatc cctccatgaa atagccgcaa gccacccgag gattctcccg catggccgac
      421 ctgagcatcg cttcgagcct gttcgaattc gcgccggaaa ttttcgcgcg cggcctgcag
      481 gcgggttgcg ccgtggcctg cgacgtcgcc ggcgcccgcg cccaattcga gccgctcgag
      541 ccgccgcgcc aggcgccggg cgaactgctc tttcaatggg tggagcgcgc atccggcctt
      601 cacgcggcga tccgcgttcg ccgcgaaccc cgcggggccg cgatcatcca gaccacgctc
      661 agcaatcccg ccgccgcggc ctcgcctccg ctcacccggc tgcgaccgtt gctgctcgaa
      721 tggcctcacg gcatggccgg gcgggtccgg ctccgttcgc tgagcggagg agtctgcagc
      781 gcggtctatc cgcccgaggc ctatcgcgtg cgcacggtcg agcgccgtta cgggggcgga
      841 cccgaggcct tccgcctcga aagccatccc accggcggct cctccaaccg cgatctcccg
      901 tttctgctgc tcagttgcga tgaagcgggg ctggtcctcg cgatggaatg gtcgggtcgc
      961 tgggtgatcg aggccggctg gggctgggag tatgaacgac ttcaccttca ggccgagatc
     1021 ccggtgcatc agctcgtgct ggcccccggg gagtctctgg aactgcccgc cgtccacctg
     1081 gtctttaccg acgccgacgg cctggccggc tgcaccaacg ccatccgcaa acatattcgc
     1141 gactgcatca tcccgcgccg ggcccatacc cacgtgccgg cgccgatcac gtatgattcc
     1201 tggttcggcg tgcaactgga cttcgacgcc cggttcctac gcgatcagat gcaggtcgcc
     1261 ggcgaagtcg ggctggaatc gtttacgatc gatgcggggt gggccaccag cagccagccc
     1321 gacggttccg actggggggg cggcaattac gaacggatcg acagcggcaa attccccaac
     1381 gggatcgtcg ccgacgcggc tcaccccgcg ggcgagctgg ggttggccct cggcttgtgg
     1441 ttcgacccgg aacaggcccc gcccgcgagc gacctggggc gcaagcatcc ggagtggttt
     1501 ctcggcgact cccaccatct cgacctgacg atccccgccg ccgcgcaata cctggaggac
     1561 ctgatcgccg gctggatcga gcgggccgac ctcggctggg tcaagtggga ttacaacatc
     1621 gtctttgacc agttcacgat cttcgagcgc catgatccga ccggaaaata cgcgtttcac
     1681 tacttcaagg cgctctacgg gatctgggat cgcctgctgg ctcgttatcc acatgtgata
     1741 atcgaacaat gcgccggcgg cggccgccgc ctcgacctgg ccacgctgcg ccgcggccac
     1801 gtccagtgga tctccgattt cacctgggat agcgatatct gccgcgcgat gcaggccaat
     1861 ctgaatctga tattgccggg ctgcctgtgc gccacggcgc tccacaccgt caagggcgcc
     1921 gcggagcccg gcctgagcga gctggacttt gtcgcgcgca tgatgggcgc ggttcagttc
     1981 atgggcgacc tgtcggtctg gccggcggac ctccgccgtc gcgcgcgccg gcttgtcgag
     2041 ctgttcaagg gctatcgcca cctgctggat cagaactact accagctcac gccgcccccc
     2101 gcgatgcccg aggacggcca ggccgtgcag ttcgtgagcg agacggggga ggagtggatc
     2161 gtcttcgttt tcgcgaatcg gcagaccggc gcgggtccgc tccggctgcg gggactccgg
     2221 ccggcggcga cctatcgcat cgaggagccg ttgagcgagc ggccgtgggg ggatgtttcg
     2281 gggagcgagc tgatggagca cggcctgcgg gtggacgccg ccgcgccgtt cacgttgctg
     2341 cgcggccagg cctgaccggc ctttcaaaat ccggcgctcg ttcctcactc gaaagttcgt
     2401 ccgcgattcg cgggatcgcc ttgatcgggt ctgccgtccc tacgggactt tgtcgcgatg
     2461 gggctcccgg tttcccgcca ctgacgtggc gggctgaaat ctgtcgtccc tgacgggact
     2521 gccccgctga ccggttcgg
//
LOCUS       DAOPCO010000422         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_32492_length_2522_cov_3.867045, whole genome shotgun sequence.
ACCESSION   DAOPCO010000422 DAOPCO010000000
VERSION     DAOPCO010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32492_length_2522_cov_3.867045"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLS90_17190"
     CDS             complement(<1..398)
                     /locus_tag="PLS90_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPK04183.1"
                     /translation="MKKVFVDSNVFLRLFIVDDEAQLKAAKHLIEEAAKGRLRLVIGP
                     PVLFEVAWTLRAAKLPRPQILQALRSIVTIPGVALIDADVVAAALELADTSGQEFSDA
                     YIVASSRKAGAGAVATFNRKHFEKLGASLH"
     gene            complement(799..1464)
                     /locus_tag="PLS90_17195"
     CDS             complement(799..1464)
                     /locus_tag="PLS90_17195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPK04184.1"
                     /translation="MSRELWTGVDRYIEERLLGPDAALEGALADSAAAGLPPIQVSPA
                     QGRLLTLLALTRGAKRILEIGTLGGYSAICLARGLGAGGRLISLELVEQHAEVARRNV
                     ARAGLAEWVEIRVGPALESLAAMEREGPEPFDLVFIDADKQTIPQYFDWALKSSRMGT
                     LIIVDNVVRDGALIDGNVNDEAIQGVRKFHEQLKGEKRVTATTIQTVGCKGYDGLTFA
                     LVV"
     gene            complement(1663..1890)
                     /locus_tag="PLS90_17200"
     CDS             complement(1663..1890)
                     /locus_tag="PLS90_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04185.1"
                     /translation="MAIQDGAQYVIRNLDVPLRLRACFEQITGKWLFFGVNDHVLLQL
                     EADGHFTPLIMYPEPAQFTVKDLELVAEPTH"
     gene            complement(2043..>2522)
                     /locus_tag="PLS90_17205"
     CDS             complement(2043..>2522)
                     /locus_tag="PLS90_17205"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPK04186.1"
                     /translation="MTKGQAAPTSRTGMRSKSSPYGTTELPVDPVWAALTMGATFVAQ
                     GASFDVKQLTDLILMGIRHPGTAVINILSPCVTYNRDMDRTYFKEHSFPLPEAYDPHD
                     YEAALLLVRRNAGRFPLGLIYVNEEEGTFDRGFETVQQLAVHKMGQADYAKILEQLI"
BASE COUNT          444 a          902 c          689 g          487 t
ORIGIN      
        1 tcgtgaagcg acgctcccag cttttcgaaa tgctttcggt taaacgtcgc cacagcccct
       61 gctccggcct tgcgggaact ggcaacgatg taggcatcgc tgaattcctg accggaagta
      121 tccgcgagtt ccaaggcggc ggcgacgaca tccgcatcga tcagtgcaac cccggggatg
      181 gtgacgatcg accgcagcgc ctggaggatc tgcggccggg gaagtttcgc ggcacgcagc
      241 gtccacgcta cctcgaacag gacaggaggc ccgatcacaa ggcgcagccg tcccttggcg
      301 gcctcttcaa ttagatgctt ggccgctttt agttgagcct catcatccac aatgaacaaa
      361 cgaaggaaaa cgttgctgtc gacgaagacc ttcttcattc gtccttgccc atcacatgtc
      421 gcgccaccgc ttcctccatc cgctttcgct ctcgcgccgc gccgacgctc ctccccccga
      481 tcccaatcaa gtcaaatata tgcggcgcct tctggattac gatccggcca tccctggcct
      541 cgatcgtcac aaggtcgccg gtctggatgc ccaaccggct ccgcaatctc ttgggcagtg
      601 tgatctgccc tttgctcgaa acagtcgcag tgctcaccat cgcccttcct atgccacctt
      661 ctttaccatc ttagggaagt atgataatct ccatcaagct ttacttttcc caagggtaaa
      721 gaaaaaacgg ttgccccccg gtcatggcaa aacttccagc gtgtcccggc acggagtggg
      781 ggcaatgggt gcacgggctc agacgaccaa cgcaaacgtc agcccgtcat accccttgca
      841 gcccacggtc tggatcgtcg tggcggtgac gcgcttctca cctttgagtt gttcatgaaa
      901 cttccgcacg ccctggatcg cctcgtcgtt cacgttgccg tcgatcagcg cgccgtcgcg
      961 cacgacgtta tcgacgatga tcagcgttcc catccgcgag gacttgagcg cccagtcgaa
     1021 gtactgcggg atcgtttgct tgtcggcgtc gatgaacacg aggtcgaagg gctccggccc
     1081 ttcccgctcc attgcggcga gcgactcgag cgccggaccg acccggatct ccacccactc
     1141 ggccaggccc gcgcgtgcga cgttcctgcg cgccacctcg gcgtgttgct ccacgagttc
     1201 cagcgagatc aaccggcccc ccgcgcccag cccacgcgcc aggcagatcg cgctgtagcc
     1261 ccccagcgtc ccgatctcca gaattcgctt cgctccgcgc gtcagcgcca gcagcgtcag
     1321 cagccgccct tgcgccggcg agacctggat cggcggcaga ccggccgccg ccgagtcggc
     1381 cagcgccccc tccagcgcgg cgtcgggccc cagcagccgc tcctcgatgt atcgatcgac
     1441 cccggtccac agttcccgcg acatgctcgc gcagctcctt ccatggcgta agctgtcgca
     1501 cgatagcagg tggacgaacc gtgggtcgag gcatcttttg gcaatgcgcg cccgcggacc
     1561 gcgccgccgc cggcccgggc tcgcccacgg tcgcccgcga tccgcgcgcg attcggccag
     1621 atcgaggagg cggcggcctc ggggcgcgtt ttgccccgcg ggtcagtgcg tcggttccgc
     1681 caccagctcc agatccttca cggtaaactg cgccggttcg ggatacatga tcagcggcgt
     1741 gaagtgaccg tccgcctcca gttgcagcag gacgtggtcg ttgacgccga agaacagcca
     1801 cttgccggtg atctgctcaa aacaggcgcg tagtcgcagc ggaacgtcca ggttgcgaat
     1861 gacatattga gcgccgtcct gaatcgccat cgcctttctc catgtctaag ttaaacagat
     1921 ccttagatct caaatcttcg cacccaaatc caccccggcc ccgctgactc ccttgagccc
     1981 tttcgtccct tgcgtccctt atgtcctctg cgggccttcg cagcttgagc cgccggcccc
     2041 cgctaaatca gttgctccag aatcttcgca tagtcagcct gacccatctt gtgcacggcc
     2101 aattgttgca ccgtttcaaa accccggtca aacgtccctt cctcctcatt cacgtagatc
     2161 agccccagcg gaaaccgccc cgcgttgcgc cgcaccagca gcagcgccgc ctcgtagtcg
     2221 tgcgggtcat acgcctccgg cagcgggaac gaatgctcct tgaaataggt ccgatccatg
     2281 tcgcggttat acgtcacgca cggcgacaga atgttgatca cggcggtccc cggatggcgg
     2341 atccccatca ggatcagatc ggtcagttgc ttgacgtcga agctcgcccc ctgcgccacg
     2401 aacgtcgccc ccatcgtcag cgccgcccac accgggtcga ccggcagttc cgtcgtgcca
     2461 tacggcgagc tcttcgaccg catccccgtc cggctcgtcg gcgccgcctg ccccttcgtc
     2521 at
//
LOCUS       DAOPCO010000423         2521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_32510_length_2521_cov_3.978913, whole genome shotgun sequence.
ACCESSION   DAOPCO010000423 DAOPCO010000000
VERSION     DAOPCO010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32510_length_2521_cov_3.978913"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLS90_17210"
     CDS             <1..378
                     /locus_tag="PLS90_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04187.1"
                     /translation="RNAEEAVEIVKLIEELLHAAPTLRLQFLRYEDYTLYANSPFTYA
                     FDPFSSPMLADGKGWRYEAAEETHGWHVQLSYGGYSSSFLRPDYQITTASNDRLVGLR
                     RWITDAREWNLRQGPKVSGGIRQ"
     gene            464..700
                     /locus_tag="PLS90_17215"
     CDS             464..700
                     /locus_tag="PLS90_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04188.1"
                     /translation="MKLTIGVPGVMLKELMRLTGARTRREAVVTAIAEFNRSKRSAQL
                     RRYIGTCRDFISLEESMRLLAFARTSPDPVEKER"
     gene            703..>2521
                     /locus_tag="PLS90_17220"
     CDS             703..>2521
                     /locus_tag="PLS90_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK04189.1"
                     /translation="MPEHKAVYSHGILSAILLSLSFFVVSNLSPASDLPRPEHPRPDM
                     QRDAWLNLNGPWEFAETDDAEQTFLGDAAYPDTIIVPFPRESELSGLNRTGFIQHVWY
                     RRTFQMPATWQGGRVRLHIGAADWRTNVWVNGQHVGEHVGGSAPFAFEITHALQPGDN
                     AVIIHCFDDTASGIQATGKQSHRPESHGAVYTRVTGIWQTVWLERVGSSFIRGLRITG
                     DPKTGEVEFSAEVDGARPGMQLWVSVLPGEEYRSDTQAFGRADADRLNTRVRLKIQNP
                     IAWSIENPHLYNAQLQLRDGDETLDVMNTYFGLREITLHGAALLINGEPVFQRLVLDQ
                     GYYPDGIWTAPTDEALRRDIELSLAAGFNGARLHQKVFEPRFLYWADRLGYLVWGEFP
                     NWGLNYGNPRGHLPVITEWTELVQRDRNHPAVIGWCPFNETDGAGPLQNALFQLTRAI
                     DPTRPVIDTSGWTHSLPREDVLVTDDHDYNQDPESFRARWGFFDPLLHSLPPRYGVKS
                     YLGTAFFLSEYGGIGWNTGEGGWGYGNNPRTLEAFYARYQGLTDVLLDNPAMFGFCYT
                     QLYDIEQERNGIYTYDRKPKFDIDRIRAVNVREAAYEKEP"
BASE COUNT          483 a          873 c          721 g          444 t
ORIGIN      
        1 cggaacgcgg aagaggcggt tgagatcgtc aaactgattg aagagctgct gcatgccgcc
       61 cccacgctcc ggctacagtt tctccgttat gaagactata cactctacgc caactcccct
      121 ttcacctacg ctttcgatcc gttctcgtct cccatgcttg cggacgggaa gggttggaga
      181 tatgaggcgg cggaggagac ccatggttgg catgtgcagc tctcctatgg aggctactca
      241 tcgtccttcc tgcgacccga ctaccaaatc acgacagcat ccaatgacag gctcgtcgga
      301 ctgcggcgct ggatcaccga tgcccgcgaa tggaatttga ggcaagggcc aaaagtttcg
      361 ggcgggatca ggcaatgaac ttcgatccgg ttttgctcgg gcgccggcag ccttgcgggc
      421 cgacggcgtc catcccgcga cggatactgg gagggcaatg cacttgaagc tgaccattgg
      481 agtcccaggc gtgatgctga aggagttgat gcgcttgaca ggggcgcgga ccaggcgtga
      541 agcggtcgtc accgccatcg cggaattcaa tcggagcaag cgttcggcgc agctcaggcg
      601 atacatcggc acgtgccgcg acttcatcag tctcgaagaa tccatgcgac tgctggcttt
      661 cgcgcgaacc tcacccgatc ccgtcgaaaa ggaaagatga ccatgccaga gcacaaggca
      721 gtttattcac acggcatcct ttcggccatc ctcctctccc tgtctttttt cgtggtctcc
      781 aacctgtctc ccgcttccga cctcccccgc cctgaacacc cgcggcccga tatgcagcgc
      841 gacgcgtggc tcaatctgaa cggcccatgg gaattcgccg agacggatga tgcggagcag
      901 acatttctgg gtgacgcggc gtatcccgac acgatcatcg tccccttccc gcgcgagagc
      961 gaactctcgg gcttgaaccg cacgggcttc atccagcacg tctggtatcg gcgcacgttt
     1021 cagatgcccg caacatggca aggcggtcgc gtgcggctcc acatcggcgc ggccgactgg
     1081 cgcacgaacg tgtgggtcaa cggccaacat gtcggcgagc acgtcggcgg cagcgcccca
     1141 ttcgctttcg agatcaccca cgcgctccag cccggcgaca acgccgtcat catccactgc
     1201 ttcgacgaca ccgccagcgg catccaggcc accggcaagc agtcgcaccg ccccgagagc
     1261 cacggcgcgg tctacacccg cgtcaccggc atctggcaga ctgtctggct ggaacgcgtc
     1321 ggctcgtcct tcatccgcgg cctccgcatc acgggcgatc cgaagacggg cgaggtggag
     1381 ttctcggccg aagtggacgg cgccagaccg ggcatgcagc tctgggtgag cgtcctgccg
     1441 ggtgaggaat atcgttccga cacgcaggcc ttcggtcggg ccgacgcgga tcgactgaat
     1501 acgcgcgtga gactgaagat tcagaatccg atcgcctggt ccatcgagaa tccgcacctc
     1561 tacaacgcgc aacttcagct tcgcgatggc gatgaaacgc tggatgtgat gaacacctac
     1621 ttcggcctgc gcgagatcac gctccacggc gccgcgctgc tcatcaacgg cgagccggtc
     1681 ttccagcgcc tcgtgctcga ccagggctac taccccgacg gcatctggac cgccccgacc
     1741 gacgaagccc tgcgccgcga catcgagctt tcgctcgcgg ccggctttaa cggcgcgcgc
     1801 ctccaccaga aagtcttcga accacgcttc ctctattggg ccgaccggct cggctacctc
     1861 gtctggggcg agttccccaa ctggggcctc aactacggca acccgcgcgg tcatctgccc
     1921 gtcatcacag agtggactga gctggtccag cgcgaccgca accacccggc cgtcatcggc
     1981 tggtgcccgt tcaacgagac cgacggcgcc ggtccgctcc agaacgcgct ctttcaactg
     2041 acccgcgcca tcgatccgac acgccccgtc atcgatacca gcggctggac ccacagcctc
     2101 ccgcgcgagg acgtcctcgt caccgacgac cacgactata atcaggatcc cgaatccttt
     2161 cgagcgcgct ggggcttctt cgatcccctc ctccactcgc ttcccccgcg ctacggcgtc
     2221 aagtcgtacc tcggcacggc ctttttcctg agcgaatacg gcggcatcgg ttggaacacc
     2281 ggcgagggcg gctggggcta cggcaacaat ccccggaccc tcgaagcgtt ctacgcccgc
     2341 taccagggcc tcaccgacgt gctgctcgac aaccccgcca tgttcggctt ctgctacacg
     2401 cagctctacg atatcgaaca ggaacgcaac ggcatctaca cctacgaccg caagccgaag
     2461 ttcgacatcg accgcatccg cgcggtcaac gtgcgcgagg ccgcttacga aaaagagccc
     2521 c
//
LOCUS       DAOPCO010000424         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_32853_length_2501_cov_5.412510, whole genome shotgun sequence.
ACCESSION   DAOPCO010000424 DAOPCO010000000
VERSION     DAOPCO010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32853_length_2501_cov_5.412510"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..615)
                     /locus_tag="PLS90_17225"
     CDS             complement(106..615)
                     /locus_tag="PLS90_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04190.1"
                     /translation="MSDYNDDDELEQGAGLEPTPASLAHYLEEQYVGDDRFETIEVLD
                     PSPHEGEAVRVCFSYDDVSQFFVAVLEEDGLVRVGLMTQEKALSERLESAAQESADSL
                     TEFLEVSLESGEELDHEVQHFHDDAFYFCSDIPYQRDDDLASEILRDEIIYYLDGYIG
                     AFYGLLDEA"
     gene            complement(816..1205)
                     /gene="rpsR"
                     /locus_tag="PLS90_17230"
     CDS             complement(816..1205)
                     /gene="rpsR"
                     /locus_tag="PLS90_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPK04191.1"
                     /translation="MAERNDERQEPGRDRAEGSERPARGEREERPFQRADRGDRGGER
                     GDRGDDRGPRGGRRPRRHRGKVCQFTVEKINYIDYKDIDRLRNYISDSGKILPRRITG
                     TSARHQRMLAVALKRARYMALLPFKVR"
     gene            complement(1368..1868)
                     /locus_tag="PLS90_17235"
     CDS             complement(1368..1868)
                     /locus_tag="PLS90_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPK04192.1"
                     /translation="MIEMNKVFLCGNLTADPDYRNTASGLGVASLRLAVNRRGRFDRE
                     TGQAQEETLFIDVTAWDKLADFSRNYLSKGRRIFVEGRLKMDQWQDKETGGNRSKITV
                     VAERIQFADSKPQGEGGGAPGGGGRAEGGSSWGRADSGGGSQAREEPSYERQGTADNT
                     EDDLPF"
     gene            complement(1928..2458)
                     /gene="rpsF"
                     /locus_tag="PLS90_17240"
     CDS             complement(1928..2458)
                     /gene="rpsF"
                     /locus_tag="PLS90_17240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPK04193.1"
                     /translation="MARKLRNYEAMVILDAGLNDQETNALIERLQKVLTDGGANLKEV
                     SRWGRRRLAYPIRKKNDAYYVIFYFTLEGSGQVLEQFERSCRFDENVYRHMVVQVPLK
                     KRGLEVAQIVPSPGYLADFRVEPRAKAMARRRASSQYDEAGEGGGAATAVEERPAPEQ
                     PSEAERPADDAASSQE"
BASE COUNT          355 a          919 c          765 g          462 t
ORIGIN      
        1 acaccggccg cgtcgtcgac gtctcaacct actggaacca cctcgcctga tccccatcgc
       61 catcgctgtc gggatcggta tcgcgagcgc aatcgccacg gcggcctacg cctcgtcgag
      121 caggccatag aacgcgccga tgtaaccgtc caggtagtag atgatctcgt cgcgcaggat
      181 ctccgaggcg aggtcgtcgt cgcgctgata cgggatgtcg ctgcagaagt agaacgcatc
      241 gtcgtggaaa tgctggacct cgtgatcgag ctcctcgccg ctctccagcg agacctcgag
      301 gaactcggtc agggaatcgg ccgactcctg cgccgccgac tccagccgct cgctcagcgc
      361 tttctcctgg gtcatcagcc ccacgcgcac caggccgtcc tcctccagca ccgccacgaa
      421 gaactggctc acgtcgtcgt agctgaaaca gacgcgcacg gcctcgccct cgtgcgggct
      481 cggatcgagc acctcgatcg tctcaaatcg gtcgtcgccg acatactgct cttcgaggta
      541 gtgcgccagg ctggcgggcg tcggttccaa gcccgccccc tgctccagtt cgtcgtcgtc
      601 gttgtagtcg ctcatggtgc tgcgctcccc acgccggccg ccgctccagc gccgcgttct
      661 cgcgaaaatc ggtaagaagc aatctacgac cgaccgtcgg cgatgtcaaa aattccctga
      721 gcgaggcgcg ccggcgccgc ccgaaaaaca aaaacccggc cgcgcgggcc gggtccttgt
      781 cgagtgccaa ttcgctgatt ccgcttttgg cgcgctcaac gcaccttgaa cggcagcagc
      841 gccatgtagc gcgcccgctt cagggcgacg gccagcatgc gctgatggcg cgcgctggtg
      901 ccggtgatcc gccggggcag gatcttgccg ctgtcgctga tgtagttgcg cagccggtcg
      961 atgtccttgt agtcgatgta gttgatcttc tcgacggtga actggcagac cttgccgcga
     1021 tggcgccgcg gacgccgtcc gccgcgcggc ccgcgatcat cgccccggtc gccgcgctcg
     1081 ccgccccgat cgccgcgatc cgcccgctga aacggacgtt cctcgcgctc gccgcgtgcc
     1141 ggccgctccg atccttccgc ccggtcgcgc cccggttcct gccgctcgtc gtttctctcc
     1201 gccatggtct ttctcctcaa tctgagcctg gtccgccgcc gagcaaggag gctgcggcgg
     1261 gggaccggaa cgacggcccc ggcgggccgg tccggtcttg gttgggggcg agccgcgacg
     1321 gcccggtccc cggctgcgat ggccggtgcg gccatccctg acatccgcta aaacggcagg
     1381 tcgtcctcgg tgttgtcggc cgttccctgg cgctcgtagg aaggttcttc tcgcgcctgg
     1441 gaaccgccgc cgctgtcggc gcggccccac gacgatcccc cctcggcacg gccgccgccg
     1501 cccggcgcgc cgcctccctc gccctgcggc ttggaatcgg cgaactggat ccgctcggcc
     1561 accaccgtga tcttcgaccg gttgccgccg gtctccttat cctgccattg atccattttg
     1621 aggcggcctt cgacgaagat gcgccgcccc ttggaaaggt agttgcgact gaaatcggcc
     1681 agcttatccc acgccgtcac gtcgataaac agcgtctctt cctgcgcctg gccggtctcg
     1741 cggtcaaacc gcccgcgacg gttgacagcc agccggaggc tcgccacgcc caagccgctg
     1801 gcggtgttgc gatagtccgg atcggccgtc aggttcccgc acaggaacac cttgttcatc
     1861 tcaatcatgg tcgccgcctc tcatcgggcc agccggcccg tggttgccgg tcggttgccg
     1921 ccccgggtta ttcctgcgaa ctggccgcgt cgtccgccgg gcgctcggct tcgctgggtt
     1981 gctccggcgc cggccgctcc tcgacggccg tcgcggcgcc gcccccctcg ccggcctcgt
     2041 cgtattgcga cgacgcgcgc cgccgggcca tcgccttcgc gcgcggttcc acgcggaagt
     2101 cggccaggta gcccggcgag ggcacgatct gcgccacctc cagtccgcgc ttcttcagcg
     2161 gcacttgcac caccatgtgg cggtacacgt tctcgtcgaa gcggcaggac cgctcaaact
     2221 gctcgagcac ctggccgctg ccctccagcg tgaagtagaa aatcacgtaa taggcgtcgt
     2281 tcttcttgcg gatcggatag gccagccgcc gccgtcccca gcgcgagacc tccttcaggt
     2341 tcgcgccgcc gtcggtcagc accttctgca ggcgctcgat cagcgcattg gtctcctggt
     2401 cgttgaggcc ggcatccagg atcaccatcg cttcgtaatt gcgcaacttc ctggccatgt
     2461 gcgacatgac ctcccttcgg tttgatcacg agtgattcaa g
//
LOCUS       DAOPCO010000425         2501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_32866_length_2501_cov_2.559280, whole genome shotgun sequence.
ACCESSION   DAOPCO010000425 DAOPCO010000000
VERSION     DAOPCO010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32866_length_2501_cov_2.559280"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..1336)
                     /locus_tag="PLS90_17245"
     CDS             complement(113..1336)
                     /locus_tag="PLS90_17245"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPK04194.1"
                     /translation="MTVKRSPVCDYDRLRAAMDRAGLDLILASSRRNVGWLTDHQTEH
                     WTWQHAILHMMEKEYDGQDYLVFAGFPRDPAKRSYLVEFAHREEAIRRRGIFADDFYG
                     YWRRGRVPEITGDGISLEQEWTRTSVEAAVQGIKDNGLEAGTIGVEWPRISAAVMEQL
                     RALLPRAKFVDAFELLFDLRQVKSAEEIRRFRQAYAVASATYRRVFERAGVGVTPREL
                     LLLEMESIYSSGCSFSFAHVFFGDGQHDIAYTPPADRAIQAGDLGLFDLGVVYEGYGT
                     DYARMAEVAPGNQKLRQYFPAILAGRRAIEDALRPGARAGDVFLAGAKVLEEHGLTAS
                     ISCLGHGIGIGCHERPFLVANSEDRIEVGQMLVIEMYAEVRGLGPVLMEDGGLMTEEG
                     WQSFGEIPTDILRLG"
     gene            1552..>2501
                     /locus_tag="PLS90_17250"
     CDS             1552..>2501
                     /locus_tag="PLS90_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04195.1"
                     /translation="MSLLCRLARRPDPNRMIPRRLACRRAPSAPCLAILSCLAALAVS
                     AAAQPIQLQNDGLAIQLDRQTGKLRSLTNRLTDETFTVQASATGLETTQGILRTGEAV
                     SADQDEHGAHFQYVAGPVAIQVDYRLPAGQDYLEKIVTVRNQSATTLTLHQVIMEELT
                     VAPAFKQIVKHHDPNLWRTLLNVFLRTEKGGFFLGVENPVMQFWSRGATIGNSVMILA
                     YPPRWLLGPGQTYVSEPAFLGAYRNEGIYLFRELAKQFDALDAPRVQPSHMNWEQQIL
                     DWGEVWAMRKFIRDIMPARDSRRPGFYVRAVAMVGGSTSST"
BASE COUNT          419 a          934 c          721 g          427 t
ORIGIN      
        1 ggatatgccg cgccgcggag gttcgccctg gcgtggccgc gggcgcccgt cccgcgcgtg
       61 ttccgtgaaa accgccggtc ttgcccgggc ggatcaggcg atccggtttt gcctacccca
      121 gccgcaggat gtccgtggga atctcgccaa aggactgcca gccctcttcg gtcatcaggc
      181 cgccatcctc catcagcacc ggccccaggc cgcgcacttc ggcatacatc tcgatcacca
      241 gcatctggcc gacctcaatg cggtcctcgc tgttggcgac caggaacggc cgctcgtggc
      301 agccgatgcc gatgccatgg cccaggcagg aaatcgacgc ggtcaggccg tgctcctcga
      361 gcactttcgc gccggccagg aagacgtcgc ccgcgcgcgc gccgggacgc agtgcgtctt
      421 caatcgcccg ccgcccggcc agaatcgccg gaaaatactg gcgcagcttc tggttgcccg
      481 gcgccacttc cgccatccgc gcataatccg tcccgtaacc ctcgtagacc acgcccaggt
      541 cgaacagccc caggtcgccc gcctggattg cgcggtcggc cggcggcgtg taggcaatgt
      601 cgtgctggcc gtcgccgaag aagacgtgcg caaaactgaa tgagcacccg ctgctgtaga
      661 tcgactccat ctccagcagc agcagttccc tgggcgtcac gccgacgccc gcccgctcga
      721 acacccggcg atacgtcgcg ctggcgaccg cgtaggcctg gcggaagcgc cggatttcct
      781 cggccgactt cacctggcgc aggtcgaaca gcagctcgaa cgcatcgacg aatttcgccc
      841 ggggcaacag cgcccgcaac tgctccatca ccgccgccga gatgcgcggc cattccactc
      901 cgatcgtccc ggcctccagg ccgttgtcct tgatcccctg caccgccgcc tcgaccgacg
      961 tccgcgtcca ttcctgctcc agcgaaatcc cgtcgccggt gatctcgggc acccggcccc
     1021 gccgccagta gccgtaaaag tcgtccgcga agatcccccg ccggcggatt gcctcctccc
     1081 ggtgggcgaa ctcgaccaga tacgaccgct tcgccgggtc gcgcgggaaa ccggcgaaca
     1141 ccaggtagtc ctggccgtcg tactcctttt ccatcatgtg caggatcgca tgctgccacg
     1201 tccagtgttc ggtctggtgg tcggtcaacc agccgacgtt gcgccgcgag ctggccagga
     1261 tcaggtccag cccggcgcgg tccatcgccg cgcgcagccg gtcgtagtcg cacactgggg
     1321 atcgcttgac tgtcatggat gcaattacct cgatttccgc cgtgggggag atggtcgaaa
     1381 gtccgtcgcc gggcgcgggt ctgaaccgcc ccttttcggg ttgacaagcg gggacttatc
     1441 gcttcagaat aagctgcgaa aacgattatc ggataacgat tgtttgtgtc aagcggagat
     1501 ttcttcgccc ccccgggacc gctcataacc cagaccagga gtgctgattc gatgagcctg
     1561 ctctgccgcc tcgcgcggcg ccccgaccca aacaggatga tcccccgccg gcttgcgtgt
     1621 cgccgggcgc cttccgcgcc ctgcctcgcg atcctctctt gtctggccgc gctggcggtc
     1681 tccgccgccg cgcagccgat tcagctccag aacgacggcc tcgccatcca gttggatcgc
     1741 cagaccggca agctccgctc ccttacgaat cgactgacgg acgaaacctt caccgtccag
     1801 gcgagcgcca ccggcctcga aaccacccag ggcatccttc ggaccggcga ggccgtttcc
     1861 gccgatcaag acgaacacgg tgcgcacttc caatacgtcg ccgggccggt cgccatccag
     1921 gtcgattacc gcctgcccgc cggccaggat tacctcgaaa agatcgtcac cgtccgcaac
     1981 cagtccgcca ccacgctgac cctgcatcag gtcatcatgg aggagctgac cgtcgccccc
     2041 gccttcaaac agatcgtcaa gcaccacgac ccgaacctgt ggcggacgct gctcaacgtc
     2101 ttcctccgca ccgaaaaagg cggcttcttt ctcggcgtcg aaaaccccgt catgcagttc
     2161 tggtcgcgcg gcgcgaccat cggcaacagc gtcatgatcc tcgcctaccc gccccgctgg
     2221 ctcctcggtc ccggccagac ctatgtttcc gagcccgcct tcctgggcgc ctatcgcaat
     2281 gaagggatat atctcttccg cgaactggcc aaacagttcg acgcgctcga cgctccgcgc
     2341 gttcagccct cgcacatgaa ctgggagcag cagatcctcg actggggcga agtctgggcc
     2401 atgcgcaagt tcatccgcga catcatgccg gcccgcgact cgcgccgccc cggcttctat
     2461 gtccgcgccg tcgccatggt cggcggcagc acctcctcca c
//
LOCUS       DAOPCO010000426         2492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_33016_length_2492_cov_5.544932, whole genome shotgun sequence.
ACCESSION   DAOPCO010000426 DAOPCO010000000
VERSION     DAOPCO010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33016_length_2492_cov_5.544932"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="PLS90_17255"
     CDS             <1..666
                     /locus_tag="PLS90_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPK04196.1"
                     /translation="SGRVVLALVFIALMHSGVVSDRGIEMPPAVFITFGVLLWMTFSE
                     AVTMPLGVVGKSRHLVTQTSIVPEALILSVVWRVAFNSIFRIAILAIVAAALGSLSPV
                     GFLKFLLLFPAIVLLGLALGILLAPFNTIYNDIGRFVNILLRPLMYASPVLYARSMHP
                     LLDTFNACSPVSIVLVNLRSLATRNVLEQPGLLASCVGMSLLFLVGWFIFHVSIPVVA
                     ERA"
     gene            668..2002
                     /locus_tag="PLS90_17260"
     CDS             668..2002
                     /locus_tag="PLS90_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPK04197.1"
                     /translation="MKPALIVENVYKKYSRNAHAHLDYGIRDLFNTLMGHRQRLALRA
                     DEFYAVENVSFQLDQGDSLGLIGRNGSGKTTLLRMCNGLIKPDGGRIMVDGRVQALIA
                     LGAGFNPKLSGRENIYNAAAVMGMNSRQTRAIFDEIVQFSELEEFIDSPIGTYSSGMY
                     ARLGFSVSVHLQPDVLLIDEILSVGDHAFQNKCFRKLHQIKKQGVTFVLVSHSQTHIV
                     QMCERAIWLHQGRLMQLGPSTEVVQAYLDFLDQENLERLREQPDGAEAAPSRPKEEKR
                     QPIPPAGARISEATRKAAADGLYHAVYDEQDRVEDVRLEFYAGDSPVDVVTMHQRLTI
                     RYSFRLRQPVTDLNVSLVFYRADGLQLSTISTLNGHLLRDFHEGQIECEVEIEDFCLV
                     PGTYVLMMPIHEGQSYLYRNVVKEFVVRSNGRLSWAVLDFPYRYRVLSKTPR"
     gene            2078..>2492
                     /locus_tag="PLS90_17265"
     CDS             2078..>2492
                     /locus_tag="PLS90_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04198.1"
                     /translation="MTLTGQIKRRLRQWVEPPPAAPADEPPPTRAAAWLRVLEDYPLE
                     ALQQERPALWDQLLAWVREEAPFSRLINEDPARDAILADESFQRAAARDPRVLWSVLI
                     TDESMKFLRRHARGIRLAAEDGWFLVQLLREERVLE"
BASE COUNT          409 a          810 c          793 g          480 t
ORIGIN      
        1 tcgggccgcg tggtgctggc gctggtcttc atcgcgctga tgcattcggg cgtggtgtcg
       61 gaccgcggca tcgaaatgcc ccccgccgtc ttcatcacgt tcggcgtgct gctgtggatg
      121 accttcagcg aagcggtcac gatgccgctc ggcgtggtcg gcaaatcgcg ccacctcgtc
      181 acgcagacga gcattgtgcc cgaggcgctg atcctttcgg tcgtctggcg cgtcgccttc
      241 aattccatct tccgcatcgc gattctggcg atcgtggcgg cggcgctcgg ttcgctctcc
      301 cccgtcgggt tcctcaaatt cctgttgctg ttccccgcga tcgtcctgct ggggctggcc
      361 ctcggcattt tactggcgcc gttcaacacg atttataacg acatcggccg gttcgtgaac
      421 atcctgctgc gcccgctgat gtatgccagc ccggtgctct atgcgcgctc gatgcatccg
      481 ctgctcgata cgtttaatgc gtgcagcccg gtctcaatcg tgctggtcaa tctgcgctcg
      541 ctggcgacgc gcaacgtgct cgagcagccg ggtctgctcg cctcgtgcgt cggcatgagc
      601 ctgctgttcc tggtcggctg gttcatcttc cacgtctcga tcccggtcgt ggcggaacga
      661 gcctgaaatg aagccggcgc tgatcgtcga aaacgtctac aagaaataca gccgcaatgc
      721 ccatgcgcat ctggattacg gcatccgcga cctgttcaac acgctgatgg gccatcggca
      781 gcggcttgcg ctgcgcgccg atgaattcta cgccgttgag aacgtctcgt ttcaactcga
      841 ccagggcgat tcgctcggcc tcatcggccg caacggctcg ggcaagacca ccctgctgcg
      901 catgtgcaac ggcctgatca aacccgacgg cgggcggatc atggtggacg gccgcgtgca
      961 ggccctgatc gcgctggggg cggggttcaa cccgaagcta tcgggacgcg agaacatcta
     1021 caacgccgcc gccgtgatgg gcatgaacag ccggcagacg cgcgccatct tcgacgaaat
     1081 cgtgcaattc tccgagctcg aggaattcat cgacagcccg atcgggacct acagctcggg
     1141 catgtacgcc cgcctcggct tctccgtttc cgtccatctg caacccgacg tgttgctgat
     1201 cgacgaaatc ctttcggtcg gcgatcatgc cttccagaac aagtgtttcc gcaagctgca
     1261 tcagatcaag aagcagggcg tgacgtttgt gctcgtctcg cacagtcaga cgcatatcgt
     1321 gcaaatgtgc gagcgcgcga tctggctgca ccagggccgg ctgatgcagc tggggccgtc
     1381 gaccgaggtt gtgcaggcgt atctggattt tctggatcag gaaaatctgg agcggctccg
     1441 cgagcagccc gatggcgcgg aggcggcgcc atccaggccg aaagaggaga agcgccagcc
     1501 catcccgccg gcgggggccc gcatcagcga ggcgacgcgc aaggccgccg ccgatggcct
     1561 ctatcacgcc gtctacgacg agcaggaccg ggttgaggac gtccggctgg agttctatgc
     1621 cggcgattcg cccgtggacg tcgtgacgat gcaccagcgg ctgacgatcc gttacagctt
     1681 ccgcctgcgc cagccggtca ccgacctgaa cgtctcgctc gtgttctacc gggctgatgg
     1741 cttgcagctc tcgaccatct cgacgctcaa cgggcacctg ctgcgcgatt tccacgaggg
     1801 acagatcgaa tgcgaggtgg aaatcgagga cttctgcctc gttcccggca cttacgtgct
     1861 gatgatgcca atccacgagg gccagagcta tctctaccgc aacgtcgtca aggagttcgt
     1921 ggtccgcagc aacggccgtc tgtcgtgggc ggtcctcgat tttccctatc gctatcgggt
     1981 gctgtcgaag actccacgct gaacgccgac cgatatttgc tggcgagcgg gggccgcctt
     2041 ccgcatgata gggctgcatg aaagtgagtg gcgacggatg acgctgacgg gacagatcaa
     2101 gcgccgactg cggcaatggg tggagccgcc gcccgcggcg ccggccgacg agcccccgcc
     2161 gacgcgggcg gccgcctggc tgcgcgtgct cgaggactat ccgctcgaag ccctccagca
     2221 ggagcgaccc gcgctctggg accagttgct ggcctgggtg cgggaggaag ccccgttcag
     2281 ccggctgatc aacgaggacc ccgcccgcga cgccatcctg gcggatgaga gttttcagcg
     2341 cgccgccgcc cgcgatccgc gcgtgctgtg gtccgtcctg atcaccgatg agtcgatgaa
     2401 gttcctgcgc cggcacgcgc gcggcatccg gctcgcggcc gaggacggct ggttcctggt
     2461 ccagctcctc agggaggagc gtgtcctcga gt
//
LOCUS       DAOPCO010000427         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_33618_length_2458_cov_4.875572, whole genome shotgun sequence.
ACCESSION   DAOPCO010000427 DAOPCO010000000
VERSION     DAOPCO010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33618_length_2458_cov_4.875572"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLS90_17270"
     CDS             <1..480
                     /locus_tag="PLS90_17270"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HPK04199.1"
                     /translation="QGQPITDFNPPEEMNHYVQGGFYGHPFVVESRIVRYEFMNKPGI
                     VELAAKTTPPAWEGGAHWAANSMTFYAGDQFPGARGDAFVAYHGSWNRSEPAGYCVAR
                     VLFDSGRPYGELVYAGFLTAEGEILGRPVDTAVAPDGSLLISDDHGGRVYRMSYTGQ"
     gene            482..1132
                     /locus_tag="PLS90_17275"
     CDS             482..1132
                     /locus_tag="PLS90_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04200.1"
                     /translation="MGAMRMAVGVVALSAAVALGCGGKAEERPSAGGVGAAAVAAGPA
                     GAAPIEPAPADPAPAPVDLETGPIEEFESACARCHGPEGAFFAGRFRHAADDGELFAI
                     TATMMRGPGMLKPTEAEFQAMADYMKALERDRPFATINNGRALLTGETAAIAGAKREG
                     TTVGVIANGQPIEVQQEGRSWTAAPGATAFVVEARAGDQATSFTYPASQWPDLLRE"
     gene            1159..1725
                     /locus_tag="PLS90_17280"
     CDS             1159..1725
                     /locus_tag="PLS90_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04201.1"
                     /translation="MKRKNGTIVRGAAAVGCAVLSAAPAWAQTPGSEVFADPLALLMR
                     WLHILAAVVAIGGAFYLRFLVLPVAARQLGAEDFAKLRQALGERWRFFVHGAVALLLI
                     SGLYNYLAVTRVQHAGQPLYHMLFGIKFLLALIVFFVAEALVSSRGWSKGLRAQARLW
                     LGLLLGVALLVILIAGVMKMMGTAAPVG"
BASE COUNT          408 a          672 c          938 g          440 t
ORIGIN      
        1 cagggtcagc cgatcactga cttcaatccg ccggaagaga tgaaccatta cgttcagggc
       61 gggttctacg gccatccgtt cgtcgtcgag tcgcggatcg tgcgctatga attcatgaac
      121 aagccgggga ttgtggagct ggcggcgaaa acgacgccgc cggcgtggga agggggggcg
      181 cactgggcgg ccaactcgat gacgttctat gccggcgacc agtttccggg cgcgcggggc
      241 gacgcattcg tcgcctatca tggttcgtgg aatcggagcg agccggctgg ctactgcgtg
      301 gcgcgcgtgc tgttcgatag cgggcgcccc tatggcgaac tggtgtatgc cgggtttctt
      361 accgcggagg gggagattct ggggcggccg gtcgatacgg cggttgcgcc cgacggatcg
      421 ctgctgatca gcgacgacca cggcgggcgg gtctaccgca tgagctacac gggccaatag
      481 catgggcgcg atgcgcatgg cggtgggcgt ggtggcgcta agcgcggcgg tcgcgctggg
      541 gtgcggggga aaggcggagg aacgtccgtc cgccggtggg gtcggggcgg ccgccgttgc
      601 cgccgggccc gcgggagcgg caccgattga accggcgccg gccgaccccg cgcccgcgcc
      661 ggtggacttg gaaaccggcc cgatcgagga gtttgaatcg gcctgcgcgc ggtgccatgg
      721 gcccgagggg gcgttcttcg cgggccggtt ccgccacgcg gccgacgacg gcgaactgtt
      781 cgccatcacg gccacgatga tgcgcgggcc ggggatgctc aagccgacgg aggctgaatt
      841 ccaggcgatg gccgactaca tgaaggcgtt ggagcgggat cgaccgttcg cgacgatcaa
      901 caacgggcgc gccttgctga ccggcgagac ggccgcgatc gcaggggcca agcgcgaagg
      961 cacgaccgtg ggggttatcg ccaacggcca gccgatcgag gtccagcagg aaggccggag
     1021 ctggacggcc gcgccgggcg cgacggcctt cgtggtggag gcgcgggcgg gcgatcaggc
     1081 gacgtcgttc acctatccgg caagccaatg gcccgacctg ttgcgggaat gagccagccg
     1141 aagcgaaagg agcggaggat gaagcggaaa aacgggacca tcgtgagagg agcggcggcg
     1201 gtcggctgcg cggtgctgag cgccgcgccg gcctgggcgc agacgcccgg gagtgaagtg
     1261 ttcgccgatc cgctggcgct gctgatgcgc tggctgcaca ttctggccgc ggtcgtcgcc
     1321 atcggggggg cgttttatct gcgctttctg gtgctgccgg tggcggcgcg gcagcttggg
     1381 gccgaggatt tcgccaagct gcggcaggcg ctcggcgagc gctggcggtt tttcgtccac
     1441 ggggccgtgg cgctgttgct catcagcggg ctctacaact atctggcggt gacgcgggtg
     1501 caacatgcgg gtcagccgct gtatcacatg ctgttcggga tcaagtttct gctggcgctg
     1561 atcgtctttt ttgtggccga ggcgctggtg tcgagccggg ggtggtcgaa ggggctgcgg
     1621 gcccaggcga ggctgtggct cgggctcctg ctgggcgtcg cgctgctggt gatcctgatc
     1681 gcgggggtga tgaagatgat ggggaccgcg gcgccggtgg ggtaggcgat tggagaataa
     1741 ataatggaaa atggaaatca ggcattagaa atcaaggaaa cgaaattcga gaatggaaat
     1801 tcgaaattcg aattttgagt tcgaaactca ttggaatgca tacattttgg aagggaaatc
     1861 atgtcgagga aaccgtttta acggttgaga aaaacaggga acgggcggtc accgggccga
     1921 ggcccggtgc tgatgtcagg gcccccgagg gggccagaaa aaatcccgct tgcgcgggct
     1981 cttaagttgt tcgctcgagc caatgcgagt cggttccatc gcgacccgga ggcgcggtga
     2041 aatagacaat gatttattcc ggtcgtccgg cctgggggct gggtcattcg tccccttcgg
     2101 ggacggggat attttgcggt tgcggcccca ggttccgctg cgctccaacc gagggttatt
     2161 ccaatggcgc cccgctgggg cggctcacgc gaatgaaatt ttcgtttgaa gatgcggttc
     2221 catctggcat ccctccggga tgcgggggag gaggggccga gtgaccggtg gtgtcgctgc
     2281 gctcaaccac cggctattct ctggcatccc tccaggatgc gggggaggag gggccgagtg
     2341 accggtggtg tcgctgcgct caaccactgg ttattctctg gcatcccttc gggatgcgga
     2401 gggggagggg ccgagtgacc ggtggtgtcg ctgcgctcaa ccaccggcta ttctctgg
//
LOCUS       DAOPCO010000428         2451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_33741_length_2451_cov_3.256260, whole genome shotgun sequence.
ACCESSION   DAOPCO010000428 DAOPCO010000000
VERSION     DAOPCO010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33741_length_2451_cov_3.256260"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1349
                     /locus_tag="PLS90_17285"
     CDS             144..1349
                     /locus_tag="PLS90_17285"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPK04202.1"
                     /translation="MNHPIKSAIAARPELSLALDAESATVRRERLAGGRIAITSPKGL
                     HPRNALLVEAAAAAAGSHALTVMEPDGMAALVLRGLAPERRVGLWHLDLFHLERAVDV
                     ARQNQIERLEIHTGSELPVLDESPDLIILHTQIDSEAGLTLELVRQAHDRLAAGGKLL
                     TCINNPRDQWLRRQLEKTFGNLTLFIKSKGGLVYSCKRTGKPGTDQDARPETRFYVKT
                     VQVKARDTELPFETCYGVFSSDGLDGGSHALLDLLKYPEPCRSILDLGCGWGAMGILA
                     AHWFGAERLVLIDANARAVEMARRNAQRHGLAERTEIRHEANLESLAREAETGVYDLV
                     LSNPPYATDYRVTELFVQAAARALRAGGEAWMVGKNNPRLEALMTAAFGNAATQRHWG
                     YDVVRAEKG"
     gene            complement(1504..2013)
                     /locus_tag="PLS90_17290"
     CDS             complement(1504..2013)
                     /locus_tag="PLS90_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPK04203.1"
                     /translation="MLNSLHDLYVEQLRDLYNAETQLVGALPRMMSAASSPQLKDAFQ
                     LHLNQTREHVTRLNQIFNQLGQSPVGEKCAAMEGLLKEGEQLMNEPGDPSVKDAALIA
                     AAQRVEHYEIAGYGTVKAYAKFLDFDEAADLLDKTLDEEGETDKALTKLATGGMFTGG
                     INKDAKKRK"
     gene            complement(2316..>2451)
                     /gene="nadA"
                     /locus_tag="PLS90_17295"
     CDS             complement(2316..>2451)
                     /gene="nadA"
                     /locus_tag="PLS90_17295"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPK04204.1"
                     /translation="PHMKRNTLEKLYLCMRDRAPAIELDEELRQRALLPIRRMLEMSR
                     "
BASE COUNT          429 a          798 c          786 g          438 t
ORIGIN      
        1 ccagtcgcgc ccaccccgaa tcgcttgcag ccgcggggcg actctggctg ctagaatgag
       61 acgcgcgggc gcgcggcgcg ggccgcacca ggaattcgcc gcgcgaacag cgaatgctgc
      121 caatcaaccc gaaggaaacc gccgtgaacc atcccatcaa atcggcaatt gccgcccgtc
      181 ccgaactgag cctggcgctc gatgcggaat ccgccaccgt tcgtcgcgaa cgactcgccg
      241 gcgggcggat cgcgatcacc tcgcccaagg gcctgcatcc gcgcaatgct ctcctcgtgg
      301 aggccgcggc cgcggcggcc gggtcgcatg cgctgaccgt gatggagccc gatggcatgg
      361 cggcgctggt gctgcgcggc ctggcgccgg agcgccgggt cggcctgtgg cacctggatc
      421 tctttcacct ggagcgcgcg gtcgatgtgg cgcgacaaaa ccaaatcgag cgcctggaga
      481 tccacacggg ttccgaactg ccggtgctgg acgaatcccc cgatctgatc atcctgcaca
      541 cgcagatcga cagcgaggcg gggctgacgc tggaactggt ccgccaggcg catgaccggc
      601 tcgccgccgg cggcaaactg ctgacctgca tcaacaaccc gcgcgaccag tggctccggc
      661 ggcaactcga aaaaacgttc ggcaacctga cgttgttcat caaatccaaa ggcggcctgg
      721 tctactcctg caagcggacg gggaagcccg gaacggacca ggacgcacgg cccgagactc
      781 gattctatgt gaagaccgtc caggtcaagg cccgcgacac cgagctgccg ttcgagacat
      841 gctacggcgt gttcagttcc gacgggctgg atggcggaag ccacgccctg ctggatctgc
      901 tgaagtaccc cgagccgtgc cgctcgatcc tcgatctcgg ttgcggctgg ggtgcgatgg
      961 gcatcctggc ggcacactgg ttcggtgcgg agcggctcgt cctgattgac gccaacgcgc
     1021 gcgcggtgga gatggcgcgg cgcaacgccc agcgccacgg tctggcggag cggaccgaga
     1081 tccgccacga ggccaacctg gaaagcctgg cgcgcgaggc cgagaccggc gtctacgacc
     1141 tggtgctcag caacccgccc tacgcgaccg attaccgcgt cacggaactg ttcgttcagg
     1201 cggccgcgcg tgcgctgcgc gcggggggcg aggcgtggat ggtcgggaag aacaatccgc
     1261 gcctcgaagc tttgatgacc gccgccttcg gcaacgccgc gacccagcgc cattggggct
     1321 acgacgtcgt gcgggccgag aaaggatgac ggaaagaggt tcccccgctg cgctgagcgc
     1381 gcttcgaagc tcccatgcaa gaatcaaaga ggcgcctctc aagttggtgg gtagttctcg
     1441 atgtgagctt tccccaagtc ccttgagagg cgcctcggcg cgcaacttgt cggcgcatgc
     1501 gggctatttg cgtttcttcg cgtccttgtt gatgccgccc gtgaacatgc cgccggtcgc
     1561 cagcttggtc agcgccttgt cggtttcacc ttcttcgtcc agcgtcttgt cgagcagatc
     1621 ggccgcctcg tcgaagtcga ggaacttggc gtaggccttc accgtgccat agccggcgat
     1681 ctcgtagtgc tcgacgcgct gcgcggccgc gatcagcgcg gcgtccttga ccgagggatc
     1741 gcccggctcg ttcatcagtt gctcgccctc cttgaggagg ccctccatcg ccgcgcactt
     1801 ctcgccgacg gggctttggc ccaactggtt gaaaatctgg ttgagtcgcg tgacgtgttc
     1861 gcgcgtctga ttcagatgca actggaacgc gtccttgagt tgcggcgacg aggccgcgga
     1921 catcattctc ggcagcgcgc ccaccaactg cgtctccgcg ttatacagat cacgcagttg
     1981 ctcgacatac agatcatgca gtgaatttaa catgagacag gattcctttc tcttctgcat
     2041 attccggtcg ggcgagggaa tccgctcctc agactctccg gcccatgacc tactgcgtca
     2101 caagtttcag gccagagcag ccggcttgag tcgctgggtg cggcatgccc ggcgaatgcc
     2161 ggcgggatgg ataaaagcgc ggtcttgtaa gccaaagttg ttcagaaaga ttgaaatcgg
     2221 cggcttgacc cgagtgaact tggggaaagt ctatcgagcc gacgggattc cctcatttca
     2281 ccccgatgta atccctacac gttcgcccgt ccgcctcagc ggctcatctc gagcatgcgg
     2341 cggatcggca gcagggcgcg ctggcgcagc tcctcatcca gctcgatcgc cggcgcgcgg
     2401 tcgcgcatgc acagatacag cttttccagc gtgttgcgct tcatgtgcgg g
//
LOCUS       DAOPCO010000429         2406 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_34575_length_2406_cov_3.904296, whole genome shotgun sequence.
ACCESSION   DAOPCO010000429 DAOPCO010000000
VERSION     DAOPCO010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34575_length_2406_cov_3.904296"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLS90_17300"
     CDS             complement(<1..625)
                     /locus_tag="PLS90_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPK04205.1"
                     /translation="MTTYLLQGGRILDPANQRDETADLWLVEGRIATRPTASLQPDEV
                     LDCRGLIVAPGLIDMHVHLREPGREDKETIASGTRAAAAGGFTSVCAIPNTTPVIDSQ
                     TGIKFITSRSQTDAVVNVFPYAAVTKGQKGEELTEFGDLLQAGAVGFTDDGRPIMNNQ
                     VMRRALEYSVTFDTLILDHCEDLNLAEGGVMREGELATRLGLRGWPSV"
     gene            complement(1313..1525)
                     /locus_tag="PLS90_17305"
     CDS             complement(1313..1525)
                     /locus_tag="PLS90_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04206.1"
                     /translation="MRKALFVTQPNSTLRLEGDGLLVVTRDDYKLDVDNPERRKVLLR
                     STKLNEWVLQWSRAFLSAEIAPVQRD"
     gene            complement(2075..>2406)
                     /locus_tag="PLS90_17310"
     CDS             complement(2075..>2406)
                     /locus_tag="PLS90_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04207.1"
                     /translation="GVERVLGAKYPLLDPNKPPRRNGAPAEMDRACQIGQVAARLRGA
                     GRADLAEGFQGFWADVRNARNTLAHAQLGFDGNDPPALDSRSIQKWVARFETLCQELA
                     GLSGGSQ"
BASE COUNT          411 a          762 c          762 g          471 t
ORIGIN      
        1 cgaccgaggg ccagccgcgc aagccgaggc gcgtggccag ttcgccctcg cgcatgacgc
       61 ccccctcggc cagattcaga tcctcgcagt gatcgaggat gagcgtatcg aaggtgacgg
      121 aatactccag ggcgcggcgc atcacctggt tgttcatgat cgggcggccg tcgtcagtga
      181 aaccgacggc gccggcctgg agcagatcgc cgaactcggt cagttcctcg cccttctggc
      241 ctttggtgac ggcggcgtag gggaagacat tgacgacggc gtcggtctgc gagcgcgagg
      301 tgatgaattt gatgccggtc tgggagtcga tgaccggcgt ggtgttggga atggcgcaga
      361 ccgaggtgaa gccgccggcg gcggcggcgc gagtgccgct ggcaatcgtt tccttgtcct
      421 cgcggcccgg ctcgcgcagg tggacgtgca tgtcgatgag gccgggggcg acgatcaggc
      481 cgcggcaatc caggacctcg tcgggctgga gtgaggcggt cggccgggtg gcgatccgtc
      541 cttcaaccag ccacagatcg gccgtctcgt cgcgctggtt ggcggggtcg agaatgcgtc
      601 ccccctgaag caggtaggtg gtcatgaaac cgtcccggtc gtggagttaa gggcgcatgg
      661 gcgccgcggg cgcggtcgcg ggccggtggg gccagcgggg tgcggcgtgt cccgggcccg
      721 gcatccgggc cgaaatccgg acatgatgtc gcaagggggg atgaacatca agccgaagct
      781 gcgggccgcg ccggggcgcg ggggcccccg gccaaggaac atgccgtggg cctcttgccc
      841 accctcttcg gccattcccc cccgtttttc ctcattccgt ccgttctttc gttgaatccg
      901 aacccatcga ttatttttta cttgagattc ctttcgcatc tctgtatcta ttcggaaaca
      961 aaagacgcgc tccgtccacc agcacgatcc gacagcattg gaaaagtaag gccgtgaggc
     1021 gcttccattt cagtctgagg ttccgtgctt tcccatgcag ggtgacgtaa acaactttgc
     1081 cattcaattc gaacttctga ccggcagtcg cccttttccc tggcaggagg agctatacgc
     1141 ccgcttcatc agcgaccgga ctgacaacat cccggacgcc tgcctgctgc cgacgggcct
     1201 gggcaagacc tccgtcatcg ccatctggct gctggcgcgt ctcgcccacg gcaggcccca
     1261 aattcccccg gtccccggac cgctcgcaaa ccgggttgca aaccgtgtgc tttcagtctc
     1321 gttgaacggg ggctatttcc gcgctcaaaa aagcgcggct ccattgaagc acccactcgt
     1381 tgagtttcgt agaccgcagc agcaccttgc gccgttccgg gttgtccacg tccagcttgt
     1441 aatcgtcgcg cgtgaccacc agcagaccgt cgccttccag gcgcagcgtg gaattgggtt
     1501 gcgtgacgaa gagcgccttg cgcatgggcc ggttccccct gatcgcgtca tcgacaacgg
     1561 ccggcgggcc cgttgctgca tgggcgaagc atgccatggc ggcgcggtgc gcagtgggtt
     1621 gtccaatatt gaatcaggcg acaggaaatc tgagcggaaa gccgacggtc ggacccgatc
     1681 gggggcacgc ttttggaccg gcaggccctg agttcggacc gagaccgtga ggggatgacc
     1741 gcctctcagg tcgtccaggt ccgcctcgga caaccggttc gctttccgat ccgcagcctt
     1801 gtctgaaatc tgtgatccgc aatcaaatgc gaagtctgaa atcggaaaac tgaaatctga
     1861 aacccgaaat gggtcgcctc ccccctcggg tgaggagggg tgactttgca acgcagtatt
     1921 accgtacaga ttagtcatgg ctcgatgccg ctcgcgcgtc gcccatgcct tttgcggagc
     1981 attgctccta ttggctttgt acgcatgcgt aacctccccc caatttcgcg taccattttg
     2041 gttacgatat cgaacatctg gcccgcaaca cgtatcattg tgatccacct gataggccgg
     2101 ccaattcctg gcagagtgtc tcaaaccggg cgacccattt ctgaatgctg cgactgtcca
     2161 acgcgggcgg atcgttgccg tcgaagccga gctgggcatg ggccagcgtg ttgcgcgcgt
     2221 ttcggacgtc cgcccagaag ccctgaaacc cttcggccag atcggcgcgg cccgcgcccc
     2281 gcaggcgcgc ggcaacctgg ccgatctggc atgcccggtc catttccgcg ggcgcgccgt
     2341 ttcttctcgg cggcttgttc ggatccagca gcggatactt cgctcccagc acgcgctcga
     2401 cccccg
//
LOCUS       DAOPCO010000430         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_35683_length_2351_cov_4.505662, whole genome shotgun sequence.
ACCESSION   DAOPCO010000430 DAOPCO010000000
VERSION     DAOPCO010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35683_length_2351_cov_4.505662"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="PLS90_17315"
     CDS             <1..413
                     /locus_tag="PLS90_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPK04208.1"
                     /translation="IFGWKDAQQFLVLRRMARDLAVPVEMIGVETVREPDGLALSSRN
                     AYLSPAERGAAPAIYRGLSVAHEAFGRGERDAAKLIAAARAEIAREPLLTIEYLEARS
                     LETLAELTRAEEGNTLLAAAVRCGSTRLIDNVRL"
     gene            490..1458
                     /locus_tag="PLS90_17320"
     CDS             490..1458
                     /locus_tag="PLS90_17320"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPK04209.1"
                     /translation="MASYLITGGAGFIGSHLAEALLARGHHVWIIDDLSTGSIANLDP
                     IRDNPRLQYFVDTIMNTARMAELIDRVDGVFHLAAAVGVRLIFENPVHTIQTNIRGTE
                     IVLELAARKKKKVQIASTSEVYGKGSRVPFRESEDLVLGPTTCPRWSYACSKLIDEFL
                     ALAYWREKQMPTLVTRFFNTVGPRQTGRYGMVIPNFVGQALRGEALTVHGDGRQTRSF
                     AHVRDVVRAVIELMERDDTNGEVFNIGNPQEVSIIDLARRILELTGSHSEIRHVPYEE
                     VYGPDFEDLDRRVPDVTKLAGTIDWSALQDLDAILRAVIETERGKL"
     gene            2042..2272
                     /locus_tag="PLS90_17325"
     CDS             2042..2272
                     /locus_tag="PLS90_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04210.1"
                     /translation="MATGKHPYLITNATEFGIFRSTDDGSTWSEVSSGLTNLDVRALV
                     VDAEGTLYAGTAGGVFRSTYLVPAELSAFSVE"
BASE COUNT          439 a          664 c          797 g          451 t
ORIGIN      
        1 cgatctttgg ttggaaggac gcgcagcagt ttctggtgct gcggcggatg gcgcgcgacc
       61 tggcggtgcc ggtcgagatg atcggcgtgg agacggtgcg cgagcccgac ggcctggcgc
      121 tcagttcgcg caacgcctat ctgagcccgg cggaacgggg ggcggcgccg gcgatctatc
      181 gcgggttgag tgtcgcgcac gaggcgtttg ggcgagggga gcgcgacgcg gcgaaattga
      241 tcgcggcggc gcgggccgag atcgcgcgcg agccgttgct gacgattgaa tacctggagg
      301 cgcggtcgct ggagacgctg gcggagctga cacgcgccga ggagggcaac acgctgctgg
      361 ccgcggcggt ccgttgcggg tcgacgcggt tgattgataa tgtcaggttg tagggaggga
      421 gaacgaatcg attgcgatga ccagggaaga aggacgagag acgagagacg atctgattga
      481 ggggaattca tggcatccta tttgatcact ggcggggcgg ggtttatcgg atcgcatctg
      541 gccgaggcgc tgctcgcgcg cggccatcac gtctggatca tcgacgatct ctcgaccggc
      601 tcgattgcga acctggaccc catccgcgac aacccccggc tgcagtactt cgtcgatacg
      661 atcatgaaca cggcgcggat ggccgagctg atcgaccgcg tggatggcgt ctttcatctg
      721 gcggcggcgg tgggggtgcg gctgatcttc gagaacccgg tccacacgat tcagaccaac
      781 attcgcggga cggagatcgt gctggaactg gccgcccgca aaaagaagaa ggtgcagatc
      841 gcctcgacgt cggaggtata cggcaagggg tcgcgcgtcc cgttccgcga gagcgaggat
      901 ttggtgctcg ggccgacgac gtgcccgcgc tggtcgtatg cgtgttcgaa gctgatcgac
      961 gagtttctgg cgctggccta ctggcgcgaa aagcagatgc cgacgctggt gacgcggttc
     1021 ttcaacacgg tcggcccgcg gcagaccggc cgctacggca tggtgatccc gaacttcgtc
     1081 gggcaggcgc tgcgcggcga ggcgctgacg gtgcatggcg acggcaggca gacgcgcagt
     1141 ttcgctcacg tgcgcgacgt ggtgcgggcc gtgatcgagc tgatggagcg cgacgacacg
     1201 aacggcgagg tgttcaacat cggcaacccg caggaggtgt cgatcatcga cctggcgcgg
     1261 cggatcctgg aactgaccgg cagccattcg gagattcgcc acgttcccta cgaggaggtt
     1321 tacgggccgg acttcgagga cctggaccgg cgcgtgcccg acgtgacgaa gctggcgggc
     1381 accatcgact ggtccgcgct tcaggatctc gatgcgatcc tgcgggcggt gattgaaacg
     1441 gagcgcggga agctgtaagt ccgccgcatg gggaatcggc ttgtggccgc ggagacgggt
     1501 gccggcatcg ccggaatacc ctcagcatcc ccggtagtga cccggcgaca aaaggggttt
     1561 acgcctcaat ccctgcttct ccgaacaatg caaggccgtt ttcatcaatg gtttgtagat
     1621 cacggatcac ggaacgtcgt tctttgtttt tattgcaata ggttcacgtc ccaggtcata
     1681 ctagatcgtt cgaaaaatag cagtagcccg gcatgaattt ccattcgtgc cggacaatcc
     1741 attacgactc gctgcaggtg cgatcggcgt tatcgccgtc ctggcacggg tcccgatggt
     1801 gagaaaggac atgtccatgg gtcacgagtg aaacgtggga agagaaactc aggaaccaat
     1861 catccattac gcaactgacc ggagacgaag atgaactctt tgcagtcttc tatgactccg
     1921 gctatcatgg catatacctc tctgttaatg gcggctccgc ttggattagg aggaatttcg
     1981 ccttcctttc agtggaaggt tacgttcacg cgatcgtccc gcggcccttc ggcgaccttt
     2041 cgtggctacc ggcaaacacc catatctcat cacaaacgcg acggaatttg gcattttccg
     2101 ctcgaccgat gacggctcga cctggtcgga agtctcttcc ggcctgacca acctcgacgt
     2161 acgggcactg gtggtggacg ccgaaggcac gctctacgcc ggaacggccg gcggcgtctt
     2221 ccgctcgacc tacctggtgc cggcggagct gtcggccttc agtgtggagt aggttgtcgc
     2281 gttccggaca taacgcgctt agcaggaccg gcatcctgcc tgtcgccgga ctcctcaagg
     2341 ttttcatcgg c
//
LOCUS       DAOPCO010000431         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_35761_length_2347_cov_3.978185, whole genome shotgun sequence.
ACCESSION   DAOPCO010000431 DAOPCO010000000
VERSION     DAOPCO010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35761_length_2347_cov_3.978185"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..2322)
                     /locus_tag="PLS90_17330"
     CDS             complement(181..2322)
                     /locus_tag="PLS90_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04211.1"
                     /translation="MLKTIFLLALGLAGLPPRAEWVEPAEAVALGAEALAGADRHGGH
                     WLDESGVIRQDQATTETALLLFEDRGVNQYSIEARVRLDGLTTGSAAGLLLQARNPNH
                     YLFYTLEQRYGGIYAVLGFVNEVSENPFPGGSSSLVEGAFIVDLSRHPELNVGQWQHL
                     RADVQGAEVVCYLNGKPAARYWFKGALPKYEKAGRLWPADPVDGRVGLYSTRCVAEFA
                     DVTIGPIQSELPLATPLRGARDQAGKLLPLRSYDETMRLLNEWMITSVEVIDPDPAPA
                     SLRGAPPYLVSNWVTSDNRYYGNIGGEYGFNHSISILGAIQYYLYSGDARALEVARNL
                     AEWHLEHRVPADAAAPHLVQSVVFWREDGSFEGMEWGLEMDKSAYMGLAFLSLHAITS
                     DPRYLQGAREIASVLREYQHEEGNWPFRINALTGEVTEAYSCSQLWYVLFFEKLHEVE
                     GDAGDLERSRKALQWLIDHPIKDNRWFGVYGDVAVGGESFDQWIPIETALYLLEHRDE
                     VVGAVQMAEDILDYLGRELIVDEGFHPDVPGLTEQTTFPVVETIQQFRLAELYAKLYE
                     ATGREEYKDAAIAAANSTTWCQMSDGKMRQGLGSLAFPCPLVLNYNFQLCRIMACIPE
                     TAPRGEDHLLHNTSLLRRIDYADSRVSYETFGPGEEILTVRRQPAEVTSGGVAMNRLP
                     VWGFHEEGWWYDEATGQLRVRHRAPRLEIRF"
BASE COUNT          440 a          798 c          690 g          419 t
ORIGIN      
        1 cggcgccggc gtggtcagca agatcatcga ataactcccg ttcgcgcctc cgtcaaatcg
       61 aaatccccgg acccgccagg gttcggggat ttttgctttg cacgctagcg gtatcggttt
      121 cgacacatcg cccgcccgca tcccgccatg ggtgccgaaa tcattggcga accgccccca
      181 tcaaaatcga atctcgagcc ggggcgcgcg gtgccgcacg cgaagctgac cggttgcctc
      241 gtcgtaccac cagccctcct catggaagcc ccacacgggg agccgattca tggcgacgcc
      301 tcccgacgtg acttccgccg gctgccggcg gacggtcagg atctcttccc ccggcccgaa
      361 tgtttcatag ctcacccgac tgtcggcata atcgattcgc cgcagcagac tcgtattgtg
      421 aagcagatga tcctcgccgc gcggagcggt ctccggaatg cacgccatga tgcggcagag
      481 ctgaaagttg taattcagca ccagggggca cggaaaggcc agactcccca gcccctgacg
      541 catcttgcca tccgacatct ggcaccaggt cgtggagttg gccgccgcaa ttgccgcgtc
      601 cttgtattcc tcgcggcccg tggcttcata cagcttggcg tagagctccg ccagccggaa
      661 ttgctgaatc gtctcgacca cggggaacgt cgtctgctcc gtaagtccgg gaacatcggg
      721 gtggaagccc tcgtccacga tcagttcgcg ccccaggtaa tccagaatat cctcggccat
      781 ctgcaccgcc ccgaccactt cgtcgcggtg ctccagcagg tagagcgccg tctcgatcgg
      841 aatccactga tcaaaggatt cgccgccgac cgcgacatcg ccatagacgc cgaaccagcg
      901 gttgtccttg atcggatggt cgatcagcca ctgaagcgcc ttgcggctcc gttccagatc
      961 cccggcgtcc ccctccactt catgcagctt ctcgaaaaac aggacgtacc agagctgact
     1021 gcacgaatac gcttcggtca cttcccccgt cagggcattg atgcggaatg gccagttgcc
     1081 ttcctcatgc tgatattcac gcaggacgga ggcaatttcc cgggcgcctt gcaggtagcg
     1141 cgggtcgctc gtaatcgcgt ggagactcag aaacgccagc cccatatagg ccgacttgtc
     1201 catctccagc ccccactcca tcccctcgaa ggaaccgtcc tcgcgccaga aaaccacgct
     1261 ctggaccaga tgcggcgccg cggcgtcggc cgggacccgg tgctccagat gccactcggc
     1321 cagattgcgg gccacctcca gcgcgcgggc gtcgcccgaa taaaggtaat actgtatcgc
     1381 gccgagaatg ctgatcgagt gattaaagcc gtattccccg ccgatattgc cgtaataccg
     1441 attatcggag gtgacccagt tactgacgag gtacgggggc gcaccgcgca gcgacgccgg
     1501 cgcggggtcc ggatcgatca cctcgaccga ggtgatcatc cattcgttca gcaggcgcat
     1561 cgtttcatcg taggaacgca gcggcagcaa cttgccagcc tgatctcggg cgccgcgaag
     1621 cggtgtcgcc agcggaagct cggattgaat cggaccgatc gtcacgtcgg cgaattccgc
     1681 gacgcagcgc gtgctgtaaa gtcccacccg gccgtcgacg ggatccgccg gccagagcct
     1741 tcccgccttt tcgtacttcg gcagggcgcc tttgaaccag taacgcgccg cgggtttccc
     1801 gttcaggtaa cagaccacct ccgcgccctg cacatccgcg cgcaggtgct gccactgacc
     1861 gacgttgagt tccgggtgcc gcgagagatc aacgatgaag gcgccctcaa cgagcgacga
     1921 acttccgccg gggaacggat tctccgaaac ttcattgacg aaaccgagca ccgcataaat
     1981 gccgccgtag cgctgctcga gcgtgtagaa gaggtagtgg ttgggattgc gtgcttgaag
     2041 cagcaggccg gcggcggaac cggtggtcag cccgtcgagt cggacgcgcg cctcgatgct
     2101 gtactgattg acgccccggt cctcgaacag cagcagcgcg gtctctgtcg tggcctggtc
     2161 ctgacgaatc acgccgctct cgtcgagcca atgccccccg tgcctgtcgg cgcccgccag
     2221 ggcctccgct cccagggcga cggcctcggc cggttcgacc cactcggcgc gcggcggtag
     2281 gcccgccagg cccagggcga gcaggaaaat cgtcttcaac atagcgtgcc tgatcatgcg
     2341 cagactc
//
LOCUS       DAOPCO010000432         2346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_35781_length_2346_cov_4.584024, whole genome shotgun sequence.
ACCESSION   DAOPCO010000432 DAOPCO010000000
VERSION     DAOPCO010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35781_length_2346_cov_4.584024"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1993
                     /gene="htpG"
                     /locus_tag="PLS90_17335"
     CDS             41..1993
                     /gene="htpG"
                     /locus_tag="PLS90_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003922000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPK04212.1"
                     /translation="MTATAETFQFQTEARQLLDLMIHSVYSHKEIFLRELVSNASDAL
                     DKLRFEALTNESLKPLTHDLHIRLEPNATERTLTIHDNGIGMNRDELIRYIGTIARSG
                     TAETMRLLREAQQEGLSAELIGQFGVGFYSSFMVADRVTLVTRRAGEEQAWRWESAGD
                     GTYTIEEAERAEPGTSVTLHLKSPDEEDGLEDFTREWVLRDTIRKYSDFVAYPIRMQV
                     EREEIERDEEGKPKEGGERKKVVKDETLNSMKAIWLRPEKEVSADEYKEFYKHLSHDW
                     SDPLAWFTLKAEGTQEFRALLYLPSRAPFDLFMRDGQHGISLYIKRVFIMNDCKELLP
                     EYLRFARGVVDSEDLSLNISRELLQRNRQIQVIRKRVTKKLLDELGAMMRDEREKYLQ
                     FWKEFGRVLKEGLFQDHEQRETLLRLAIFHSTHEDSAWTTLEDYVGRMKDGQDTIYYM
                     TGESLEALKRSPHLEAFRDKGIEVLLMTDPVDEVWTQSVFEFKEKKFASAGKGEVELG
                     GEEERKAAEEKRKEQAEGLKSLFEALRAPLQDHIKEVRLSSRLTRSPACLVGDQHDLS
                     PQLEQLLRASGQAIPTVKRILELNPEHPILEKLKAIHQQDANDPRLGQYAELLYGQAL
                     LAEGGKLPDPAAFSKRIEELMTQTLG"
BASE COUNT          496 a          648 c          786 g          416 t
ORIGIN      
        1 gacgcctttt ttggatgaaa tcctggatgg agattgaacg atgactgcga ccgccgagac
       61 tttccagttt cagaccgagg cccggcagct cctggacctg atgatccatt cggtctattc
      121 gcacaaggag atcttcctgc gcgaactggt ttccaacgcg tccgatgcgc tggacaagct
      181 gcgtttcgaa gcgctgacca atgaatcgct caagccgctg acccatgacc tccacatccg
      241 gctggagccg aatgcgaccg agcggacgct gacgatccac gacaacggca tcgggatgaa
      301 ccgcgacgag ctgatccgct atatcggcac gatcgcccgc tcggggaccg ccgagacgat
      361 gcggctgttg cgcgaggcgc agcaggaggg gctctccgcc gagttgatcg gccagttcgg
      421 cgtggggttc tactccagtt tcatggtggc cgaccgggtg acgctggtga cgcgccgcgc
      481 gggcgaggag caggcctggc gctgggaatc ggccggcgac ggcacctaca cgatcgagga
      541 ggccgagcgc gccgaacctg gcacgtcggt cacgctgcac ctgaagagcc ccgacgagga
      601 ggacggcctg gaagacttca cgcgcgaatg ggttctgcgc gacacgatcc ggaaatactc
      661 cgatttcgtc gcctacccga tccggatgca ggtggaacgt gaggagatcg aacgcgacga
      721 ggaaggcaaa ccgaaggagg ggggcgagcg caagaaggtc gtcaaggacg agacgctcaa
      781 ctcgatgaag gcgatctggc tgcggccgga gaaggaggtc agcgcggacg aatacaagga
      841 gttctacaag cacctgagcc acgactggag cgatccgctg gcgtggttca cgctcaaggc
      901 cgaagggacg caggagttcc gcgcgctgct ctacctgccg tcgcgcgccc cgttcgatct
      961 gttcatgcgc gacggccagc acggcatcag cctctacatc aagcgcgtgt tcatcatgaa
     1021 cgactgcaag gagctgctgc cggaatattt gcgtttcgcg cgcggggttg tggattcgga
     1081 ggatctgtcg ctgaatattt cgcgagaact cctgcagcga aaccgccaga ttcaggttat
     1141 ccgcaagcgc gtgacgaaga agctgctcga tgagctgggg gcgatgatgc gcgacgagcg
     1201 cgagaaatac ctccaattct ggaaggaatt cggccgcgtg ctgaaggagg gcctgttcca
     1261 ggatcacgaa cagcgcgaga cgctgctcag gctggcgatt ttccactcga cgcacgagga
     1321 ctctgcgtgg accaccttgg aggactacgt cggccggatg aaggacggcc aggatacgat
     1381 ctactatatg accggtgagt cgctcgaggc gctgaagcgg tcgccgcatc tggaagcgtt
     1441 ccgcgacaag gggatcgagg tgctgctgat gaccgatccg gtcgacgagg tgtggacgca
     1501 gtcagtcttt gaattcaagg agaagaagtt cgcatcggcg ggcaagggcg aagtggaact
     1561 gggcggcgag gaggagcgca aggcggccga ggagaagcgc aaggaacagg ccgaggggct
     1621 gaagtcgctg ttcgaggcgc tgcgggcgcc gctgcaggat cacatcaagg aggtgcggct
     1681 gtcaagccgg ctgacccgtt cgccggcatg cctggtgggc gaccagcacg acctgagccc
     1741 gcagctcgag cagttgctgc gcgccagcgg gcaagcgatt ccgaccgtga agcgcatcct
     1801 ggagctgaat ccggagcatc cgattctgga gaagttgaaa gcgattcatc aacaggacgc
     1861 gaacgatccg cggctggggc agtacgccga gctgctttac gggcaggccc tgctggccga
     1921 ggggggcaaa ctgccggacc ccgccgcgtt cagcaaacgg attgaggaac tgatgaccca
     1981 gacgctgggg tgacggggcg gataatcagt gtcaacatag cgccgcgaga ttccccgtca
     2041 ggcgaatggc gtgtgagtgc agtagcagaa cagttggcgt tcaagtatgt taccgcctct
     2101 tcgagaatgt aaccgtgttt ctcctgattt atgcgatctt tggttggatc gaagtcaacg
     2161 cgtgaacggc cgaaaacaat gcgaaacgcc ccatcaggta ttcccagctt gtcaacgatg
     2221 tcgctgtctc ggattgtcat ttccgatggt ctgccacgtc cctctttgag ggaatgatcc
     2281 gtatcaatgc ccggagggct tcgttgaccg cttgagaggt ggggaaatat tcccttacgt
     2341 ccggtt
//
LOCUS       DAOPCO010000433         2333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_36025_length_2333_cov_4.286655, whole genome shotgun sequence.
ACCESSION   DAOPCO010000433 DAOPCO010000000
VERSION     DAOPCO010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36025_length_2333_cov_4.286655"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..824)
                     /locus_tag="PLS90_17340"
     CDS             complement(3..824)
                     /locus_tag="PLS90_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04213.1"
                     /translation="MTGLLAGMLCLLAQAVSASTLFFEDFKDNGANVAPGLLQYNWDD
                     NNHDMVVTETRTGELIEFNTTANGWHFNSYSFDYPASDTLTWDASQHQYLQAYFPWKN
                     DSDLIALQAKIDGGGITGDMWANRLDNAQQWYHTQPTLVDVNAMFGLTGVHEIQFRFF
                     FISTQYAADAPNEHIVEGTWDWVRGGMRAGANINIAKYCVGWPAAGGRILRRRSCALP
                     HAVAWRPGAVARDRQHADRLRRFHHRLPGRVPGSGGGLPRSLLAALRLLVLEVVF"
     gene            1521..>2333
                     /locus_tag="PLS90_17345"
     CDS             1521..>2333
                     /locus_tag="PLS90_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04214.1"
                     /translation="MICIHPPLRRAGLLQRCRAAWILPMITCLLPFAGCAQPGLIGYW
                     KLRGDSRDYSGRGHHAVNHGVALGPDGAMFDGLNHYLEVPDSSDLHLGASDFTISAWV
                     KCTGGVRNVPGDILNKYDPVGRRGINLHVAASSPGYCSLSDQRNLQFGIDNAVEGTWE
                     DCGRPWPSNTLVSTLVVFDGELYTGIADADRPEDTCRMFRYAGGQEWVDCGRVGLNDM
                     KNVSVFSTVVHQGRLYAATGNWDWEKTWHGRGVTTADLARVCRYAGGTTWEDC"
BASE COUNT          436 a          768 c          674 g          455 t
ORIGIN      
        1 gttcaaaata cgacttccaa taccagtagc cgtagagccg ccaataggga tcgaggtagc
       61 ccccctcctg aacctggaac acgtcctgga agccgatggt ggaagcgacg taggcgatcg
      121 gcgtgctgac gatctcgggc cactgcacct ggccgccatg ccactgcgtg gggtagcgcg
      181 catgatcgac ggcgtaggat tcgaccgcca gccgcaggcc agccaacaca gtacttggcg
      241 atattgatat tggcccccgc acgcatgccg ccccgcaccc agtcccaggt tccctccacg
      301 atgtgctcat tgggggcatc ggcggcatat tgagtcgaga tgaagaagaa gcggaactgg
      361 atttcgtgga cgccggtcag gccgaacatc gcgttgacgt caaccagcgt cggctgcgtg
      421 tgataccact gctgcgcgtt gtcgaggcgg ttggcccaca tgtcgccggt aatcccgccg
      481 ccgtcgatct tggcttgcag cgcaatcagg tcgctgtcgt tcttccacgg gaaatacgcc
      541 tgcaggtatt gatgctgcga cgcgtcccag gtcagcgtgt cagacgccgg gtagtcgaag
      601 ctgtaggagt tgaagtgcca gccgttggcg gtcgtattga actcgatcag ttcaccggtc
      661 cgggtctccg tcacgaccat gtcgtgattg ttgtcgtccc agttatattg caaaaggccc
      721 ggcgccacat tggccccgtt atccttgaaa tcctcaaaaa agagcgtcga ggcgctcacg
      781 gcttgcgcca gcaggcagag catgccggcc agcaggcccg tcattcggat tgttctccac
      841 atgggaagct tcccctctcc tggtatatag gaaacatctc acaggcaata cagcgacagc
      901 tctcagcctt gcgcggactc ggacacctcc tttcgggatc gactgagcgg agcagacatc
      961 ccgcggacct caactcaggg cgatcggttt caggatcttt aggtgcgaaa tgaaccgtct
     1021 cttgcgggcg gtcggcggtt gggcgcggcc cggaaggatg gcttcctatt tatcatgcga
     1081 taaattagtt cagttcggct cacctgtcaa cccttttatt tcagcctgtc gattgcgccg
     1141 gaacgggccc gaatcctgtc gatggcgatg caaattaaat attatcaata ttttctatcg
     1201 taacagcggc cctctggacc aggtaaggat catgacttcc aggttcttga attgcgtgac
     1261 aaaacatcaa gatgccccat cccgcctcga atcttgcacc gcgatgcgtc attggacctc
     1321 tccgcggcgg ggtccctccg tatattccca tccccccccc gccaaccggt ccctgcgccc
     1381 cggcccgcgc ggattgacct tgcagcccgg ccggcattgg cgcatatatg ggagccacga
     1441 accccgttta tcaggtgatc tatcccagcc ggtttgaagc cttatctgtc caaactccat
     1501 gaagggacgc gatttatcac atgatctgca ttcatccgcc gctgcgccgc gccggcctgc
     1561 tgcaacgatg tcgcgccgcc tggatcctgc ccatgataac ctgcctcctc ccctttgccg
     1621 gctgcgccca gcccggtctg atcggttact ggaaactgcg cggcgatagc cgggattact
     1681 ccggccgcgg gcaccacgcc gtcaatcacg gcgtcgcgct gggccccgac ggcgccatgt
     1741 tcgacggcct gaatcattac cttgaagtcc ccgactccag cgacctccat ctgggcgcct
     1801 ccgacttcac gatctccgcc tgggtcaagt gcacgggggg cgtgcgcaac gtcccgggcg
     1861 acatcctcaa caaatacgat ccggtcggcc gccgcggcat caatctccac gtcgccgcca
     1921 gctcgcccgg ctactgctcg ctcagcgatc agcgcaacct ccaattcggc atcgacaacg
     1981 ccgtggaggg gacctgggag gattgcggcc ggccctggcc gagcaacacg ctggtcagca
     2041 cgctggtcgt cttcgacggc gagctctaca ccggcatcgc cgacgccgac cggcccgagg
     2101 acacctgccg catgttccgc tacgccggcg gccaggaatg ggtcgattgc ggccgcgtcg
     2161 gcctgaacga catgaagaac gtctcggtct tcagtacggt cgtccaccag ggccggctct
     2221 acgccgccac cggcaactgg gactgggaga agacctggca cggccgcggc gtcaccaccg
     2281 ccgacctggc gcgcgtctgc cgctacgccg gcggcacgac ctgggaggac tgc
//
LOCUS       DAOPCO010000434         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_37473_length_2266_cov_2.708729, whole genome shotgun sequence.
ACCESSION   DAOPCO010000434 DAOPCO010000000
VERSION     DAOPCO010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37473_length_2266_cov_2.708729"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLS90_17350"
     CDS             <1..625
                     /locus_tag="PLS90_17350"
                     /inference="COORDINATES: protein motif:HMM:NF019146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HPK04215.1"
                     /translation="LATQHTFQVLTKRPERMRDYMLDPAMNNRFGMARARIFDAHSVC
                     DCDVWMSGQQFRPLPNVWLGVSVEDQATDNDRIPLLLQTPAAVRWISAEPLLGPVDLT
                     RALVRSDGLQSHEWRNLLTGEIYSESPAHGHRVFGTAERIDWVVVGGESGPSWRSCSV
                     DWIEDIHDQCKAAGVPVFIKQAAGPKPGMQGRIPDDLWSVKEFPLAA"
     gene            737..2116
                     /locus_tag="PLS90_17355"
     CDS             737..2116
                     /locus_tag="PLS90_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007636513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HPK04216.1"
                     /translation="MSKGLIIDNFAGGGGASTGIEAALGRAVSIAINHWGAALDVHRA
                     NHPETTHYVEDVFKVRPLEVTQGQPVDLAWFSPDCKHRSKAKGGKPVDRQIRGHAWVA
                     VKWADQVRPRVIILENVEEFRDWGPLDADGKPCKRRKGMFYKRFCTRLSNLGYQVEDR
                     ILTAADYGAPTTRTRLFLVARCDGQPIVWPEPTHSRDGASDLFGSRKPWRTAAECIDW
                     SIPCASIFERKRPLAENTLRRIAEGLRRYVIDSADPFIVGIDNKSSGTGAAWGTDEPL
                     RTITQENRFALVCAFLSKHHGGVVGHSLRQPLGTITGVDHHSLTTAHLTKFYGTNIGA
                     DLRQPLPTITATGQHIAEVRAFLVSYYGQGVGQSLRSPMRTITTKDRLGLVTVRGVLY
                     QIADIRLRMLQPHELALAQGFPASYILPLNKSLAVALIGNSVCPPLAEALVRANLPEQ
                     AVALEAAAS"
BASE COUNT          420 a          716 c          752 g          378 t
ORIGIN      
        1 gcttgcgact cagcacactt ttcaggtgct tacgaagagg ccggagagaa tgcgcgatta
       61 catgctcgat ccagccatga acaataggtt tggcatggcc agggcgcgta ttttcgatgc
      121 tcattccgtt tgcgactgcg acgtatggat gtcaggacag caattccgtc cgctgccaaa
      181 cgtctggctt ggcgtgtctg tcgaggacca agccaccgac aacgatcgca tcccgctgtt
      241 gctgcagacg ccggccgcgg tgcggtggat cagcgcggag ccgctgctcg ggccggtgga
      301 cctcactcgc gccctggttc gtagtgacgg cctgcaatcc catgagtggc gaaatctgct
      361 gactggcgag atttacagcg agagcccggc gcacggtcat agagtattcg gcaccgcgga
      421 gaggatcgac tgggtcgtcg tcggcggcga gagcgggccg agctggagat cctgtagcgt
      481 tgactggatt gaagacatcc atgatcagtg taaagcggcg ggcgtgcccg ttttcatcaa
      541 acaggcggct gggccaaagc cggggatgca gggccgaata ccagacgatc tctggtcggt
      601 gaaggagttt ccgctcgcgg catgaaaaaa ggccggccca tgaggaagaa tcccggccgg
      661 cccggccgcg gcgccagggg cggatgctgg agagcaccct gaagcgcggc gatccagctt
      721 agaaatgagg ggttgaatga gcaagggctt gatcatcgac aacttcgcgg ggggcggcgg
      781 cgcgtcgacc ggcattgagg cggcgctggg gcgagcggtg tcgatcgcga tcaatcattg
      841 gggcgcggct ttggacgtcc accgggcgaa ccacccggag acgacgcact atgtcgagga
      901 tgtattcaag gtccggccgc tcgaagtgac ccagggccaa ccggtagacc tcgcatggtt
      961 cagccccgac tgcaaacacc gctcgaaggc caagggcggg aagccggtgg accgccagat
     1021 ccgcgggcac gcatgggtcg cggtcaaatg ggccgatcag gtccggccgc gagtcatcat
     1081 cctggagaac gtcgaggagt tccgcgactg gggtccgctg gacgccgatg ggaagccgtg
     1141 caagcggcgc aagggcatgt tctacaagcg gttctgcacc cggctcagca atctgggcta
     1201 tcaggtcgag gatcgcatcc tgacggcggc agactacggc gcaccgacga cgcggacccg
     1261 cctgttcctc gtcgcccgct gtgacggaca gccgattgtc tggcctgagc cgactcacag
     1321 cagggacggc gcgtcggatc tgttcggttc gagaaagcct tggcggaccg cggccgagtg
     1381 catcgactgg agcatcccct gcgcgtcgat attcgagcgc aagcgtccgc tggctgagaa
     1441 cacgctcaga cgcatcgcgg agggactcag gcgttatgtc atcgactcgg ctgatccatt
     1501 tatcgtgggc atcgacaaca agagcagcgg cacgggcgcg gcgtggggca cggatgagcc
     1561 gcttcgcacg atcacgcagg agaaccgatt cgcgctggtc tgcgcattcc tctcgaagca
     1621 ccacgggggc gtcgttgggc acagcctcag acagcctctc ggaacgatta caggcgttga
     1681 ccaccattcc ctcacgacgg ctcacctgac gaaattctac ggcacgaaca tcggcgcaga
     1741 cctgcgccag ccgctgccca ccatcaccgc caccggccag catatcgccg aagtccgcgc
     1801 cttcctcgtc tcctactacg gccagggcgt cggccagtcg ctgcgctcgc ccatgcggac
     1861 gatcacgacg aaggaccggc tggggctcgt gacggtccgg ggcgtgctct accagatcgc
     1921 cgacatcagg ctcaggatgc tccagcccca cgagctggcc ctcgcgcagg gattcccggc
     1981 atcctatatc ctaccgctca acaagtcgct ggccgtggcc ctgatcggga actcggtctg
     2041 tccgcctctg gccgaggccc tcgtgcgcgc gaacctgccc gagcaggctg ttgcgctgga
     2101 ggcggcagca tcatgagccg tttcaccagc cccgcctacc ccgttcacac gcaccgcgac
     2161 cgcgacgtcg ccgatacgat ccggccggcc gatccgatca cgtgcgacga ctgcgcgcgg
     2221 ccgtggcagc cgggggctgt gttctggcga cgtgcttccc ggccgg
//
LOCUS       DAOPCO010000435         2258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_37664_length_2258_cov_4.260554, whole genome shotgun sequence.
ACCESSION   DAOPCO010000435 DAOPCO010000000
VERSION     DAOPCO010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37664_length_2258_cov_4.260554"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(392..>2258)
                     /locus_tag="PLS90_17360"
     CDS             complement(392..>2258)
                     /locus_tag="PLS90_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04217.1"
                     /translation="ILNFHNIRLTPIIHRKVTFAEQVRREALAWRPDVIAVELPATLA
                     SWIVRGALRLPQISAVVFEEQGAPGELSYLPIDPCDGLIEAVRLACEHGIPLEFIDLD
                     LAGLQEPWRYLPDDTMLDLTGLEPFVDRVAPLVATRPDDRTSLARERHMALRLRELAA
                     RHRRVLAVLGLGHFTRVRRLLEADHIDPPAAALGPGDPPIVAQRPDARLVHVSPASLS
                     HLLGEIPYLTWLFEQSREELELTGEVRFDKFAALQTIFKLAEHEYRQRYKETITLTQW
                     KALLQFTRNMALVRGGLRPDLYEIVLGARGVVDGDFGCEVLEIARSYPPQRKNDEALP
                     NFRIRNGRGLLEGRRERFRLRPRQEEPPSELVQLRFRRRPPAELKARWKEQWQRSAGR
                     GICSWPPEDERQERFMDYVRKRALQVVSEDRRQVQEFTTSMLDGLDIRETMRNWHTGK
                     LFVQTTPQPQGKVSAVVLVFQDEPIGELGSWRATLYAENQNESDISFYASPLGENVVG
                     PRIARTEFGGILSIYPALGIPDIWRFELPDEIRTCADALLAAGILFSSERYVAYVAAR
                     PPRQVLKRLAEIYRKHIIYMPLHGFSANHLKAIRKFHILDGHDVRQWAADYIFDD"
BASE COUNT          339 a          653 c          894 g          372 t
ORIGIN      
        1 gcgggggagg aggggccgag tgaccggtgg tgtcgctgcg ctcaaccacc ggctactctc
       61 tggcatccct tcgggatgcg gagggaggag gggccagcga ggggggggcg gtctcacatg
      121 ggccaggatg cggtcggaat ctccggccac cccttgcagg ggttcggggc ggttggggcg
      181 gcgacgcagg gttccggcct gcggccgtca ccctgcgctt tattccggtc gtccgctgcg
      241 cggacttggg cattcgcctc cttcggggat ggggatattt tgcgggggtg tccccaggct
      301 ccgctgcgct ccaaccgagg gttattccaa tggcgccccg ctggggcggc tcacgcgggt
      361 gaaattcgca tcgacgaggg acgagggggc gtcagtcgtc gaagatgtaa tcggcggccc
      421 attggcggac gtcgtggccg tcgaggatgt ggaatttgcg gatcgccttg aggtggttgg
      481 ccgaaaagcc atggagcggc atgtagatga tgtgcttgcg gtagatttcg gcgaggcgct
      541 tgaggacctg gcgcggcggc ctggcggcga cgtaggcgac atagcgctcg gatgaaaaga
      601 ggatcccggc cgccagcagg gcgtcggcgc aggtgcggat ctcgtcgggc agctcgaagc
      661 gccagatgtc ggggatgccg agcgcggggt agatggagag gattccgccg aattccgtgc
      721 gcgcgatgcg cggcccgacg acgttctcgc ccagcgggga ggcatagaac gagatgtcgc
      781 tctcattctg attttcggcg tagagcgtcg cccgccagga gccgagttcg ccgatcggtt
      841 catcctgaaa gacgaggacg acggcgctga ccttgccttg cggctgcggc gtcgtctgga
      901 cgaagagctt gccggtgtgc cagttgcgca tcgtctcgcg gatgtcgagg ccgtccagca
      961 tcgaggtcgt gaattcctgg acctggcggc ggtcctcgct gacgacctgc agggcccgct
     1021 tgcggacgta gtccatgaac cgttcctgcc gctcgtcctc ggggggccag gagcagatgc
     1081 ccctgccggc cgagcgttgc cactgttcct tccagcgggc cttcagttcg gcgggggggc
     1141 ggcggcggaa gcgcagttgc acgagttcgg agggcggttc ctcctggcgg ggccggaggc
     1201 ggaagcgctc gcggcggcct tcgagcaggc cgcggccgtt gcggatgcgg aaattgggca
     1261 aggcctcgtc gttcttgcgc tgcggggggt agctgcgcgc gatttcgagg acttcgcagc
     1321 cgaagtcgcc atcgacgacg ccgcgcgcgc cgagcacgat ctcgtagagg tcggggcgga
     1381 gcccgccgcg gacgagggcc atgttgcgcg tgaactggag cagcgccttc cattgggtca
     1441 gcgtgatcgt ttccttgtaa cgctggcgat actcgtgctc ggcgagcttg aagatcgtct
     1501 ggagggcggc gaatttgtcg aagcggacct cgccggtcag ctccagttcc tcgcggctct
     1561 gctcgaagag ccaggtcaag taggggatct cgcccaggag gtgcgagagc gaagcgggcg
     1621 acacgtggac aagccgcgcg tcggggcgct gggcgacgat cggcggatcg ccggggccta
     1681 gggccgccgc cggcggatcg atgtgatcgg cctcgagcag gcggcgcacg cgcgtgaaat
     1741 gaccgagccc caggaccgcc aggacgcggc gatggcgggc ggcgagttcg cgcaggcgca
     1801 gggccatgtg gcgctcgcgg gcgagcgagg tccggtcatc ggggcgcgtc gcgacgaggg
     1861 gcgccacgcg gtcgacaaac ggctccaggc cggtcaggtc gagcatcgtg tcgtcgggca
     1921 ggtagcgcca tggttcctgg aggccggcca ggtccagatc gatgaactcg agggggatcc
     1981 cgtgttcgca ggcgaggcgg acggcctcga tcaagccgtc gcacgggtcg atggggaggt
     2041 aggagagttc gcccggggcg ccctgttcct caaagacgac ggcggagatc tgggggagac
     2101 ggagcgcgcc gcggacgatc caggaagcga gggtcgcggg cagttcgacg gcgatcacgt
     2161 cgggccgcca ggccagcgcc tcgcggcgca cctgctcggc gaaggtcacc ttgcggtgga
     2221 tgatgggcgt caggcggatg ttgtggaagt tcaggatc
//
LOCUS       DAOPCO010000436         2219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_38568_length_2219_cov_3.466266, whole genome shotgun sequence.
ACCESSION   DAOPCO010000436 DAOPCO010000000
VERSION     DAOPCO010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38568_length_2219_cov_3.466266"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLS90_17365"
     rRNA            complement(<1..448)
                     /locus_tag="PLS90_17365"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1196..>2219)
                     /locus_tag="PLS90_17370"
     CDS             complement(1196..>2219)
                     /locus_tag="PLS90_17370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPK04218.1"
                     /translation="APNTRYHLLAPPNDPLYDQQADLNPLGELAADGDMHVQAAWGIR
                     NSAENVVVAIIDSGMQITHEDLSPNLWTNQAEANGEPGVDDDGNGYVDDLHGWNAVDD
                     NPELSDSLFHGTRVAGVLGARGDNGIGVAGTCWRIQMLPVRAFSDQETSSEILLAALD
                     YVLGIPEVRIINASWGSGEDNEALYEAFRVVQARGIMAVCGAGNGGHNNDEEPFYPGS
                     YGLDNIISVAAVNLTGELASFSNYGSSVEVAAQGSDVLTTDPYAGYYRAMGTSFATPR
                     VTGAVALMMAEFPGMSASVIRNRIIETSRWTAALAGASLMGGLIDFAPLLEATPTTVG
                     RWTAYP"
BASE COUNT          387 a          742 c          636 g          454 t
ORIGIN      
        1 ttcctgccga cagcggttta caatctcacg accttcatcc cgcacgcggc gtcgctcgtt
       61 cagggtttcc cccattgacg aagcctctag actgctgccc cccgtaggag tctgggccgt
      121 atctcagtcc cagtgtggct gaccatcctc tcagaccagc taccgatcgt cgccttggtg
      181 agccgttacc tcaccaacaa gctaatcggg gacgggccga tctcgaagcg accgaagcct
      241 ttaaccaacc agtccgggga ccggttggtc acatgcggta ttagcacgag tttccccgtg
      301 ttatccccca cttcgaggca cgttacccac ctgttactca ccagttcgcc gctttactcg
      361 ggggccgaag ccccctttct cgctcgactt gcatgcctaa tccacgccgc cagcgttcgt
      421 tctgagccag gatcaaactc tccgatggaa acctgtttcc atcccgggcc tttcggcccg
      481 gccggagaat ttcaacgaat tggtcggcca ggtttccgcc ttccaatccg tgttgcttgc
      541 tttacttggg cacctggttt ctcaaaaccg caaaggtttg ctccaagggc gcctcgcttt
      601 gcctggcgcc ctctctattc agttttcaaa gaacgacccg agtcctgcga ccccaacggc
      661 ggctcacttc cggccgcttc gggcccccgg aatgtctttt ccggtcaccc gcaaaacccg
      721 cattatacgg catcgctccg ctttgtcaag ccctcaaaat catcgtcttg aatggctttc
      781 ggaggattct ccgcctgcgg gccgccgcgc ttccgaccca cgggtcgtca ggcacagcga
      841 ggggatatca tacgggaatt ccctcacttg tcaacaccct ctttgaatga tttttttgat
      901 gacgataaac cttctgaaat cagtagtcta tgactgaaac aggcgcgatt tcagcacccg
      961 cggcggtatt ttttttcgca cgtcgaccct ggggaagctt cctcgcttcc tctttcttcg
     1021 caccgctggc gcggttccct gtggacaaaa tggccggtta tcgcacgccg gggacgaggg
     1081 gacgagagcc gagagccgag aactgatatt ttggaggcgc cggaggccgg tcacgaggac
     1141 gaggacgtga gacgaggcac ggattcgaga atgaggacga gaacgatcgg cgatttcagg
     1201 gatacgcggt ccagcggccg accgtggtgg gcgtggcctc cagcaacggg gcgaaatcga
     1261 tcaggccccc catcaggctg gcccccgcca gcgccgccgt ccagcgcgag gtctcaatga
     1321 tccggttgcg gattaccgag gccgacatcc cgggaaattc cgccatcatc agcgcgaccg
     1381 cgccggtcac ccgcggcgtg gcgaatgacg tccccatcgc gcggtaatac cccgcgtagg
     1441 gatcggtcgt cagcacgtcg ctcccctggg ccgccacctc cacgctcgaa ccgtaattgg
     1501 agaaactggc cagttccccg gtcaggttca ccgccgcgac cgaaatgata ttgtccaggc
     1561 catagctgcc cggatagaac ggttcctcgt cgttattgtg gccgccattg cccgcgccgc
     1621 agaccgccat gatgccccgg gcctggacga ccctaaaggc ctcgtagagc gcctcattgt
     1681 cctcgcccga cccccaactg gcgttgatga tccgcacctc ggggatcccc agcacgtagt
     1741 cgagcgcggc cagcagaatt tcggagctgg tttcctggtc gctgaacgcg cgcaccggca
     1801 gcatctggat ccgccagcag gtgcccgcga cgccgatccc gttgtcgccg cgcgcgccga
     1861 gcacgccggc gacccgcgtt ccgtgaaaca acgagtcgct cagctcgggg ttgtcatcga
     1921 ccgcgttcca gccatgaagg tcgtcgacgt agccattgcc gtcgtcgtcc acgcccggct
     1981 cgccgttcgc ctcggcctgg ttggtccaca ggttggggct cagatcctca tgcgtgatct
     2041 gcatcccgct gtcgatgatc gccaccacca cgttttcggc cgagttgcgg atcccccagg
     2101 ccgcctgcac atgcatgtcg ccgtcggcgg ccaactcgcc cagcggattg aggtcggctt
     2161 gctggtcgta gagcgggtcg ttgggcggag cgagcaggtg gtagcgggtg ttgggggcg
//
LOCUS       DAOPCO010000437         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_38610_length_2217_cov_5.287234, whole genome shotgun sequence.
ACCESSION   DAOPCO010000437 DAOPCO010000000
VERSION     DAOPCO010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38610_length_2217_cov_5.287234"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1228)
                     /locus_tag="PLS90_17375"
     CDS             complement(377..1228)
                     /locus_tag="PLS90_17375"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPK04219.1"
                     /translation="MIPLDRLIKKVEDLPPLPDIVVKLLHASRDPNVSTRDMVELIKH
                     DPALTIKVLRLCNSSYYGLPRKINSIQEALVYIGTDTLVNFVLAGCLSSFYQQAQDGY
                     GLSKGELWRHSVGCAIASQRIASRDNPDVTGQAFTAGLIHDIGKILLDAYVGQEIETI
                     LRLVEEESISFAEAEKRVLGFSHTEAGCELARHWNLPEPLIEAIAYHQMPDAAKLAPK
                     LVAQVHLGNILCISFGIGVGSDGLAYTFHPHALAAIGMEVSDLFRLSLDVHDDYKKAE
                     ELIGLAA"
     gene            complement(1225..1806)
                     /locus_tag="PLS90_17380"
     CDS             complement(1225..1806)
                     /locus_tag="PLS90_17380"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPK04220.1"
                     /translation="MSNKLILPNGTSQLVQPREERAAPSMSSAEGTQSSFVVVDLADM
                     KATNHPTGCLVTQSLGSCVAVAIFDPEARIGGLLNYILPDSHICSERAMTNPFLFADT
                     GIPLLFRRAYKLGALKERIVTKVAGAGNVLDPKENLRLGQQNYEALSRIMESNHVELK
                     GKFVGGFKGMALKMYLETGRVVVELGDGQEVEI"
     gene            complement(1958..>2217)
                     /locus_tag="PLS90_17385"
     CDS             complement(1958..>2217)
                     /locus_tag="PLS90_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPK04221.1"
                     /translation="LGFGLPGICVDTHVHRITNRWGYVATRTPEETEMALRERLPAEY
                     WIPMNDWLVTWGQQVCRPISPFCSRCALSRGCGRVGVARAR"
BASE COUNT          388 a          734 c          654 g          441 t
ORIGIN      
        1 tcgcttcccc aactcaatgc gcgcggattt caggttctaa tttgacacgg agcatgttca
       61 gggctgcatc gacgtcgtct tgagaagaag tcgctgagcg aatccgattt tcgaggtgta
      121 ggggagtggt ccggcgcggc cggggacatg gcagcggtca cctgacggtt tttgcgaaaa
      181 atcccaggtt ctggctcggc ccgccctgtc gttctgcccg cctcgccctt gtccactgcc
      241 ctgcatgcgg gggtaccatc cagcatccgt tcccccgtcg ccgtctccgt gagccaggga
      301 gacggcctca ctccagaatc gtccctcgtc cctggctctc gaaagcccgc tattctgaat
      361 tctggtcctc agtccttcac gcggccaggc cgatcagctc ctcggccttc ttgtaatcgt
      421 cgtgcacatc gagcgacagg cggaacaggt cggaaacttc catcccgatt gccgccaggg
      481 cgtgcggatg gaaggtgtag gccaggccgt cgctgcccac gccgatgccg aagctgatgc
      541 aaaggatatt gcccagatgg acctgggcca ccagcttcgg cgccagcttc gccgcgtcgg
      601 gcatctggtg ataggcaatc gcctcgatca gcggctcggg cagattccag tggcgcgcca
      661 gctcgcagcc ggcttcggtg tggctgaaac cgaggacccg cttttcggct tcggcgaaac
      721 tgatcgattc ctcctcgacc agccgcagaa tcgtttcgat ttcctgcccg acgtaggcat
      781 cgagcagaat cttgccgatg tcgtggatca gtccggcggt gaaggcctgg ccggtgacgt
      841 cggggttgtc gcgcgaggcg atgcgctggc tggcgatggc gcaaccgacc gaatggcgcc
      901 acaattcgcc cttgctcagc ccgtaaccat cctgcgcctg ctgataaaac gaggagaggc
      961 agccggccag cacgaaattc accagcgtgt cggtgccgat gtaaaccagc gcttcctgaa
     1021 tcgaattgat tttgcgcggc aggccgtaat acgacgaatt gcacagccgc aacactttga
     1081 tcgtcaatgc cgggtcgtgc ttgatcagct ccaccatgtc gcgcgtcgag acgttcgggt
     1141 cgcggctggc atgcagcagc ttgacgacaa tgtccggcag gggcggtaag tcctcaactt
     1201 tcttgatcag acggtccagc ggaatcatat ctcaacctct tgtccgtcgc ccaactcgac
     1261 gacgacgcgt ccggtttcca ggtacatctt caaggccatg cccttgaaac ccccgacaaa
     1321 ctttcccttc agctcgacgt ggttggattc catgattcgg ctcagcgcct cgtagttctg
     1381 ctggcccaga cgcagattct cctttggatc caacacgttg cccgcgccgg ccaccttggt
     1441 gacgatccgc tccttgagcg cccccagctt gtaggcccgc cggaacagca gcggtatgcc
     1501 ggtatccgcg aacaggaacg ggttggtcat cgcacgttcg ctgcagatgt gcgagtcggg
     1561 caggatatag ttgagcagtc ccccgatgcg ggcctcggga tcgaagatcg ccacggcgac
     1621 gcacgacccg agcgactgcg tcaccaggca tcccgtcgga tggttggtgg ccttcatgtc
     1681 ggccaggtcg accaccacaa aactggactg ggttccctcg gcggatgaca tgctcggcgc
     1741 cgcccgttcc tctcgcggct gcaccagttg actggttccg ttgggtagaa tcaacttatt
     1801 tgacatctca attcaccctg actcaatacg tcaacccttt cgggccgtgc gcgcggctac
     1861 cccctccgaa cctatcggca actcgggcga gcgacatgcg tcattttttt ggggttgacc
     1921 ctcgcggggc ggcccgccgg cccgtgcgac cggcgcctca ccgcgcccgc gccaccccca
     1981 ctcgcccgca gccgcggctc agcgcgcagc gcgagcagaa tggcgagatc ggccggcaga
     2041 cctgctggcc ccatgtcacc agccagtcat tcatcgggat ccaatactcc gccggcaggc
     2101 gctcgcgcag cgccatttcg gtctcctccg gcgtccgcgt cgcgacgtag ccccaacggt
     2161 tcgtgatccg gtggacgtgc gtgtcgacac agatccccgg caggccaaat ccgagcg
//
LOCUS       DAOPCO010000438         2215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_38663_length_2215_cov_3.860185, whole genome shotgun sequence.
ACCESSION   DAOPCO010000438 DAOPCO010000000
VERSION     DAOPCO010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38663_length_2215_cov_3.860185"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1573
                     /locus_tag="PLS90_17390"
     CDS             143..1573
                     /locus_tag="PLS90_17390"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HPK04222.1"
                     /translation="MTTRLSPRPELREFAPPAADRWRAPFGGRRLSDAITRSEGYAGH
                     ADPYSLFEEMEEKDPHLFSLLQTRKLGVLARPRKIEPAGETERDRNVAQWIERVLRAA
                     PGFDQALLHLLGALAHGIAILEILWGVDAAGRIVPRAFKPRAARRFTRDADGEWRLLS
                     DPLGWNRPETARPLPPRKFLTALSGASDEAPFGRGLCEKVYWLWWFKKHNLKFWLIYN
                     EKFGAPAIVAQHRAGLNPAERERLLEVIDALQTDAGVIVPEGITLSLLEATRAGGGDS
                     YRMLAQWCNDEMARAILGQTLTSGEGERAGSLALGRVHDAVRRDYQRADAWLLMDVVN
                     AQLVRWLVDLNFGEHVPAPRWAIDLSPELDLEREVAIDRQLLQMGVPLPLRYFHEKYG
                     RPAPVGEAPRLQYDDSNLFQYHLQFGILTINEVRVKLGLPPVPWGDAPTSPVDVRARP
                     PIPRGSTGEDPPEREFEEEVGKANEK"
     gene            1712..>2215
                     /locus_tag="PLS90_17395"
     CDS             1712..>2215
                     /locus_tag="PLS90_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04223.1"
                     /translation="MHDQLELEVFRAGDYGAKGVWDEAALERLAADYDPALHEAPVTL
                     DHAQAGPALGWVASVRRVGDRLIARLRGLNRRLLELIRRGAFRKRSIELYTALRETGR
                     PYLRAVSFLGAAAPEVKGLTDPLPAGQAGPLFAETGDDAGAGDGVVLFDDCRPPAALP
                     TEKPMENT"
BASE COUNT          375 a          780 c          741 g          319 t
ORIGIN      
        1 gcattcattt tatgttgctg caagggttca taagggcctc taagggaatt cacgcgccat
       61 ggcggtcggg attatgattg ccccatcgag cgcgagcggt ttcacgccgc cctcaccgac
      121 cccgccgacg gagacccgcc ccatgaccac ccgtctctcc ccccgcccgg aattgcgcga
      181 attcgcgccc cccgccgcgg atcgctggcg cgcgccgttc ggcggccgcc ggctcagcga
      241 cgcgatcacg cgcagcgagg gctacgccgg tcatgccgac ccctattcgc tcttcgagga
      301 gatggaagag aaggacccgc acctgttcag cctgctgcag acgcgcaagc tgggcgtgct
      361 ggcgcggccg cgaaaaatcg agccggcggg cgagaccgag cgggaccgga acgtcgccca
      421 gtggatcgag cgcgtgttgc gcgcggcgcc cggcttcgac caggcgctgt tgcacctgct
      481 cggcgcgctg gctcacggca tcgccatcct ggagatcctg tggggcgtcg acgcggcggg
      541 acggatcgtg ccgcgcgcgt tcaaaccgcg ggcggcgcgg cgcttcaccc gcgacgccga
      601 cggcgagtgg cgcctgctga gcgaccccct cggctggaac cggcccgaaa cggcgcggcc
      661 gctgcccccg cgcaagttcc tcaccgcgct cagcggcgcg agcgacgagg cccccttcgg
      721 ccgcgggctg tgcgaaaaag tctactggtt gtggtggttc aagaagcaca atttgaaatt
      781 ctggctgatt tacaatgaaa aattcggcgc gccggccatc gtggcccagc accgcgccgg
      841 actcaacccc gccgaacgcg agcgcctgct cgaggtgatc gacgccctgc agaccgacgc
      901 cggcgtcatc gtccccgaag gcatcacgtt gagcctgctc gaggcgacgc gcgccggcgg
      961 cggcgacagc taccgcatgc tggcgcagtg gtgcaacgac gaaatggcgc gcgcgatcct
     1021 gggccagacg ctcacctccg gcgagggcga gcgggccggc tcgctggccc tcggccgtgt
     1081 ccacgacgcc gtccgccgcg attaccagcg cgccgatgcc tggctgctga tggacgtggt
     1141 caacgcacag cttgtgcgct ggctggtcga tctgaacttc ggcgagcacg tgcccgcgcc
     1201 gcgctgggcg attgacctgt cgcccgagct cgacctggag cgcgaggtgg cgatcgaccg
     1261 ccaactcctc cagatgggcg tccccctgcc gctccgatat ttccacgaga aatatggccg
     1321 gcccgcgccg gtgggcgaag ccccccggct gcaatacgac gacagcaacc tgttccagta
     1381 ccatctgcaa ttcgggatcc tgacgatcaa cgaagtgcgc gtcaagctcg gcctgccgcc
     1441 ggtcccctgg ggggacgcgc cgacctcgcc ggtggacgtg cgagcccgcc cgccgatccc
     1501 ccgcggttcc accggcgagg acccgcccga acgtgaattc gaagaggaag tcggaaaagc
     1561 caatgaaaag taaagccggg tcgcatccat ccagcacggg gcaaggatca ataacggtac
     1621 ttgatcaacc aacaaatgta atagatggaa ccgacgttgg ataagcaccg gaccgcgaat
     1681 gccacgacag gccacgaaag gagaacgagc catgcacgat caattggaac tggaggtctt
     1741 ccgcgcgggc gattacggcg ccaagggcgt ctgggacgaa gccgccctcg agcgcctcgc
     1801 ggcggactac gaccccgcgc tgcacgaggc gccggtgacg ctcgaccacg cgcaggcggg
     1861 ccccgcgctg ggctgggttg cgtcggtccg tcgcgtcggc gaccggctga tcgcccgcct
     1921 gcgcgggctc aatcggcggc tgctggagct gatccgccgc ggcgcgttcc gcaagcggtc
     1981 gatcgagctc tataccgcgc tgcgcgagac cggccggccc tatctgcgcg cggtcagctt
     2041 cctcggcgcg gcagccccgg aggtcaaggg cttgaccgat ccccttcccg ccggccaggc
     2101 cggtccactg ttcgccgaga ccggcgacga cgcgggcgcc ggcgacggcg tggtgctgtt
     2161 cgacgattgc cgtccgcccg ccgcgctgcc gaccgaaaag ccaatggaaa atacg
//
LOCUS       DAOPCO010000439         2201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_38993_length_2201_cov_3.614632, whole genome shotgun sequence.
ACCESSION   DAOPCO010000439 DAOPCO010000000
VERSION     DAOPCO010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38993_length_2201_cov_3.614632"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PLS90_17400"
     CDS             <1..432
                     /locus_tag="PLS90_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04224.1"
                     /translation="NRFVDRPWMPAGEDAANAATRVVTLLLLAAWFGTALARRRASPA
                     GDDALFWAAALLMLLAPSLMWDHYAVQALPALMWLAGAPRRGPARATALLLILALLML
                     PWNYAAPPWRSGAGLLLMSIRLWPTLALYAWLLLESRHDAN"
     gene            complement(486..1205)
                     /locus_tag="PLS90_17405"
     CDS             complement(486..1205)
                     /locus_tag="PLS90_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04225.1"
                     /translation="MRFGHDHEPEVDLEARRREELRLSEGLAPLRELRPPAELRVRNR
                     RRIQAMLEELRIGNRLARRTPWWRRRMTLPWPVAVCAAALVVGLIGLDARRWLEGPAA
                     GPPTDVQSGQAGAGMETNGAKAAEATALGGAWWRMEASPVIVDGGRLIGRVTYRGGAG
                     FVVIAPGVGRWEVAAGPFEGALPAGRIENGVLRAGDLEIFGVGSGLAEGATVWVRRSR
                     EAGSGGGKGGPVRIEPAGAIR"
     gene            complement(1389..2015)
                     /locus_tag="PLS90_17410"
     CDS             complement(1389..2015)
                     /locus_tag="PLS90_17410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPK04226.1"
                     /translation="MTRADSTAEARVFDVAPRGIGGKPPEAAGGPEMTEEALLEGLRV
                     GTLEAYEVLMERFEAPLYRFFYYSHGHHDLAQDQCGETFINLLSAIQKMRGDAGCLKA
                     FIFGVARNVLRRGWRQRRLYSASEAMLARLSDARPSAYRLAASRQEYQRAMAAIEELT
                     EPARQVFLLRFVEELSLAEISEALDLPLGTVKSYIHRSRKRLLEMLRE"
BASE COUNT          317 a          997 c          540 g          347 t
ORIGIN      
        1 aaccgcttcg tcgatcgacc ctggatgccc gccggcgagg acgccgccaa cgccgccacg
       61 cgcgtcgtca cgctgctcct gctggcggcg tggttcggga ccgccctggc ccgccggcgc
      121 gcgtcgcccg ccggcgacga cgccctcttc tgggccgccg ccctgctgat gctcctcgcc
      181 ccgagtctga tgtgggatca ctatgccgtg caggccctgc cggcgctgat gtggctcgcc
      241 ggcgctcccc ggcgcggccc cgcgcgcgca accgcgctcc tcctcatcct ggcgctgctg
      301 atgctcccgt ggaattacgc cgccccgccc tggcgctccg gcgccggcct cctcctgatg
      361 tcaatccgcc tctggcccac cctggccctc tacgcctggc tcctcctcga atcccgccac
      421 gacgctaact agcggcctcg ccggtcctcc cccttttcgt gcccgtttgt gcctttcgtg
      481 atcccctatc gaatggcgcc cgccggttcg atccgaaccg gcccgccttt cccgcccccc
      541 gaacccgcct cccggctcct ccgcacccac acggtcgcac cctccgccaa ccccgaaccc
      601 acaccgaaaa tctccagatc acccgcccgc aacaccccgt tctcgatccg gccggcgggc
      661 aacgccccct cgaacggccc cgccgcaacc tcccaacgcc ccacccccgg cgcgatcacc
      721 acaaaccccg ccccgccgcg gtaggtcacc cgcccgatca gccgcccgcc gtccacgatc
      781 accggactgg cctccatccg ccaccacgcc ccccccaacg ccgtcgcctc ggcggccttc
      841 gccccgttcg tctccatacc cgcccccgcc tggccgctct gcacatccgt cggcggcccc
      901 gcggccggcc cctccaacca ccgccgcgcg tcgagcccga tcaggccgac caccagcgcc
      961 gccgcgcaga ccgccaccgg ccacggcagc gtcatccggc gccgccacca cggcgtgcgc
     1021 cgcgccagcc gattgccgat ccgcagttcc tcgagcatcg cctggatccg ccggcgattg
     1081 cgcactcgca gctcggccgg cggccgcagt tcacgcaacg gcgccagccc ctcgctcagc
     1141 cgcagctcct cgcgccgccg cgcctccaga tccacttccg gttcgtggtc gtgtccgaat
     1201 cgcaaattac cctcctcgcc tcacgcgtcc ctcatccctc gtcctcgtcc ccgtaatcgc
     1261 ccccaaaggc ctaccccaaa attcggctct cgtccctcgt cctcggctct cgaaaacccg
     1321 cccttttcca tccgtcgttc tgaaccgccg attcctgcgc acctggcagc tctttaaatc
     1381 ctcccccctt actcccgcaa catctccagc aaccgcttcc ggctccggtg aatgtaactc
     1441 ttcaccgtcc ccaacggcag atccagcgcc tcgctgatct ccgccagcga aagctcttcc
     1501 acgaatcgca gcagaaagac ctgccgcgcc ggctcggtca gttcctcgat cgccgccatc
     1561 gcccgctgat actcctggcg gctggccgcc agccgatacg ccgacggccg cgcgtcgctc
     1621 aaccgcgcca gcatcgcctc gctcgcgctg tagagccgcc gctggcgcca tccccgccgc
     1681 agcacgttgc gcgccacccc gaaaatgaac gctttcaggc accccgcatc gccgcgcatc
     1741 ttctgaatcg ccgacagcag attgatgaat gtctcgccgc actgatcctg cgccagatca
     1801 tgatgcccat gcgaataata gaaaaaccga tacagcggcg cctcgaaccg ctccatcagc
     1861 acttcatacg cctccagcgt gccgacccgc agcccctcca gcaacgcctc ctcggtcatc
     1921 tccggccccc cggcggcctc cggcggcttc cctccaatgc cccgcggcgc cacgtcgaac
     1981 acgcgtgcct cggctgttga atcggcgcgt gtcaagtccc cgacctcttc cttccggttc
     2041 cgccctcaag cgggaaccac accttcccgc cgtcccagcc cttccctccg gttccggcgg
     2101 cggcaatgcg ggaactatac gcccccgccg ccccacgcct tccccagctc aggtgccgcg
     2161 atcccaagaa aagttgcgcc ccccgaaaaa atcatacccc a
//
LOCUS       DAOPCO010000440         2164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_39895_length_2164_cov_3.411569, whole genome shotgun sequence.
ACCESSION   DAOPCO010000440 DAOPCO010000000
VERSION     DAOPCO010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39895_length_2164_cov_3.411569"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PLS90_17415"
     CDS             complement(<1..865)
                     /locus_tag="PLS90_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04227.1"
                     /translation="MNPVCHGTPRNPCLNSNMRFAMMNAFLSHLASARHGIRLITPLA
                     VLIFLFFIAVRSEAQGIPSSAWWPEYHHDSHRTGFTPLPGRIEEPVELWSYYLGAPSA
                     DLVTDATAAAATYDLDGRGGQYRVQTTTTSLIVTDAASGAEVWRRALTPYFNGYSFRV
                     GKTDPSIPGLQVAVWPATWFESTHEGNFGYLFGFGDGVAAGRELWQVELSAAAIKVPN
                     LLIADIDHDGIGDVVSVSWGLVTVWDGASGRLRYKIETNPGRNYGMAAAVNVDDDPYQ
                     EIVILADYVPHV"
     gene            complement(865..>2164)
                     /locus_tag="PLS90_17420"
     CDS             complement(865..>2164)
                     /locus_tag="PLS90_17420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPK04228.1"
                     /translation="DSLARVVNRSVGGAIGNWSSVPVAIDADGDGRMEIAIMNGRNQV
                     ELFKADGAGAPSRVWSLSGHGMVQYPGYTKANMMPAMADLDGDRRHEIIFAGASANGL
                     ATLAVHRADGGLIWEHVFDGAGFDGLYGSVERWFFGSFVGSPVRDVAVLFSRTGDNSS
                     EIAVLDGEDGSLAWARRTIGEPPLRGIFSPNLLPVTGDFTAGGADEIAYAWGSIAYVI
                     DGSDGSLLQAASLPQVFYYSGAYYSFATLLRNAGDPAPRLLWHIGNFMGLQEFDMTGV
                     WSHVLTAGNTFPPGLADADGDGVFEIGDAGADGVFRCYRADDGEPLWSAAVGQIPQSY
                     VGVADVNHDGVDDYFFTTTSGNLTVLRSQVAPGQNRLLWQHPIGGGQHPIFCDVDADG
                     AGEFLVSTADGKLKCIDDVFDRPLIEQPPLQSAASMGSYE"
BASE COUNT          414 a          670 c          737 g          343 t
ORIGIN      
        1 cgacgtgggg cacatagtcg gcgagaatga cgatctcctg gtacgggtcg tcgtcgacgt
       61 tgacggcggc cgccatgccg taattccggc ccggattcgt ctcgatcttg tagcgcaggc
      121 ggccgctggc gccgtcccag actgtgacga gcccccagga aacggaaacc acatcgccga
      181 tgccgtcgtg atcgatatcg gcgatgagga ggttggggac cttgatggcc gccgcggaga
      241 gctcgacctg ccacagctcg cgccccgcgg cgacgccgtc gccgaagccg aaaaggtaac
      301 cgaaattccc ttcatgggtg gattcgaacc aggtggccgg ccagacggcg acctgcaggc
      361 cggggatgga cgggtcggtc ttgcccaccc ggaaactgta tccattaaaa taaggggtca
      421 gcgcgcggcg ccagacctcc gcgccgctgg ccgcgtcggt cacgatcagc gaggtcgtcg
      481 tagtctgaac gcgatactgg ccgccgcggc cgtcgaggtc ataggtggcg gcggcggcgg
      541 tggcgtcggt caccagatcc gcggagggag cgcccagata gtagctccac agctcaacgg
      601 gctcctcgat ccggcccggc agcggggtga atcccgtgcg gtgggagtca tggtggtact
      661 ccggccacca ggcggaggat ggaattccct gtgcttcgga cctgacggcg atgaaaaata
      721 ggaaaatcag gaccgccagc ggagtgatca gcctgatgcc atggcgcgca gaggccaagt
      781 gggaaaggaa ggcgttcatc atcgcaaacc tcatgttgga gttcaggcac ggattgcgcg
      841 gcgtgccgtg gcatacggga ttcattactc atagctcccc atggatgccg cggactgaag
      901 cggcggttgc tcgatgaggg gtcgatcgaa cacgtcgtca atgcacttga gtttgccatc
      961 ggcggtcgag acgaggaact cgccggcgcc gtcggcatcc acgtcgcaga agatcgggtg
     1021 ctggccgccg ccgatcggat gttgccagag caggcgattc tggccggggg cgacctgcga
     1081 ccgcaggacc gtcaggttgc cggacgttgt cgtgaagaaa taatcgtcga cgccatcatg
     1141 attgacatcg gcgacaccga catagctttg cgggatttgc ccgacggcgg ccgaccagag
     1201 gggctcgccg tcgtcggcgc ggtagcagcg gaaaacgccg tcggcgccgg cgtcgccgat
     1261 ttcgaaaacg ccgtcgccgt cggcatcggc cagacccggc ggaaacgtgt tgccggccgt
     1321 cagcacgtga ctccacacgc cggtcatgtc gaattcctgc agacccatga aattgccgat
     1381 gtgccagagg aggcgcggcg ccgggtcgcc ggcgttgcgg agcaatgtgg cgaaggagta
     1441 atacgctccg ctataataga agacctgcgg gagcgacgca gcttgcagca gcgacccatc
     1501 cgaaccgtcg atcacgtagg cgatactgcc ccaggcatag gcgatttcgt cggcgccgcc
     1561 agcggtgaag tcgccggtga cggggagcag attgggcgaa aagattccgc gcaacggggg
     1621 ctcgccaatc gtccgccgcg cccaggcaag cgatccgtcc tcgccatcca gcacggcgat
     1681 ctcgctgctg ttgtcgcccg tccggctgaa gagaaccgcg acatcgcgga cggggctgcc
     1741 gacgaacgag ccgaaaaacc agcgttcgac ggagccgtac agtccatcga agcccgcgcc
     1801 gtcgaagacg tgttcccaga tcaggccgcc gtcggcccga tgaaccgcga gcgtggcgag
     1861 cccgttggcc gacgcgccgg cgaagatgat ctcatgccgc cgatcgccgt caagatcggc
     1921 catggccggc atcatgttgg ccttcgtata gccgggatat tgaaccatgc catggcctga
     1981 tagggaccac acccgggagg gggcccccgc gccgtcggct ttgaacagct cgacctgatt
     2041 gcggccgttc atgatggcga tttccatccg accgtcgccg tcggcgtcga tcgccacggg
     2101 gaccgaggac cagttgccga tggcgccgcc gaccgaccga ttaacgacgc gggcaagcga
     2161 atcg
//
LOCUS       DAOPCO010000441         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_40063_length_2157_cov_3.441484, whole genome shotgun sequence.
ACCESSION   DAOPCO010000441 DAOPCO010000000
VERSION     DAOPCO010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40063_length_2157_cov_3.441484"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLS90_17425"
     CDS             complement(<1..443)
                     /locus_tag="PLS90_17425"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017427280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HPK04229.1"
                     /translation="MPAPLRDLRQFIDLLRRERELVEIAAPVDPDQELAEIHRRVIAA
                     DGPALLFTQVKGSPFPVVTNLFGTPRRVDLAFGPRPIRFVEDAVRLARELVPPTPGKL
                     WRARGFALQAMRVGQRRARSAPVLAHLRRPPRLTDLPLVTSWPDDG"
     gene            744..>2157
                     /locus_tag="PLS90_17430"
     CDS             744..>2157
                     /locus_tag="PLS90_17430"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPK04230.1"
                     /translation="MATTTQEELSSRYDPHAIEPAILDRWNEGRLYEARPESAAEPYT
                     IVIPPPNVTGFLHIGHALNNTLQDILTRWRRMQGRDALWVPGTDHAGIATQHVVERRL
                     WNEEKKDRRDLGREAFLERVWAWKEQYGTRIINQLRRLGCSCDWSRTRFTMDEGLSRA
                     VLTVFKQLYEEGLIYRGDYLVNWSPALQTALSDDEVVHKEVRGHLWHIRYPLADGSGS
                     IEVATTRPETMLGDTAVAVHPEDERYRGMIGKQVALPLMGRSIPIIADPLVEREFGTG
                     AVKVTPAHDPNDYEMGRRHNLEFINILNPDGTLNANAGAYAGLAVAEARKRVVADLEA
                     QGLLVKVEDHVHAVGHDYRSGCVVEPYLSKQWFVRMRPLCEPAVEAVRAGRVRFVPEH
                     YANIYYHWMENVRDWCISRQLWWGHRIPIFYCGACEHVWCEIERTPARCPRCDAEQVT
                     QDPDVLNHVSSTSGSCVTCSA"
BASE COUNT          370 a          612 c          851 g          324 t
ORIGIN      
        1 ccgtcgtcgg gccaactggt gacgaggggc agatcggtga ggcgcggggg gcggcgcaga
       61 tgggcgagga cgggggccga acgcgcgcgg cgctggccga cacgcatcgc ctgaagggcg
      121 aagccgcgcg cgcgccagag cttgccgggg gtcggcggga cgagttcgcg ggccaggcgc
      181 acggcgtctt cgacgaagcg gatgggccgc gggccgaagg ccaggtcgac gcggcggggc
      241 gtgccgaaca aattagtgac gacggggaac gggctgccct tgacctgggt gaagaggagg
      301 gcgggtccgt cggcggcgat gacgcggcgg tggatttcgg ccagttcctg atcgggatcg
      361 accggggcgg cgatctcgac gagttcccgc tcgcggcgga gcaggtcgat gaactgacgg
      421 aggtcgcgca ggggagcggg catcccccct ctatagcatg gcgggggccg gaatcaaatt
      481 gcggagtgag gccgtcccgg gcggcggggg agcatcggtg acgtcgagcg agacgtgagg
      541 ggaggccgat tgatggcggg cggtagcgat ctcgcaagcg attgcgatac cgataccgat
      601 cgatcaggct cggactcgac agttgcgttg ggtttctcta tgctatgggc agggctggcc
      661 tggttgggga aggggagatg aacgagggac ccattcccgc cggccgtccg acctgttcat
      721 ccccaacctg gaaccgcgcg cgcatggcga ccacgaccca agaggaactc tccagccggt
      781 atgatccgca tgcgatcgag ccggcgatcc ttgatcggtg gaacgaaggg cggctgtatg
      841 aagcgcggcc cgagtcggcc gccgagccgt acacgatcgt catcccgccg cccaacgtaa
      901 cgggctttct gcacatcggc catgcgctga acaacacgct gcaggacatc ctgacgcgct
      961 ggcggcgaat gcaggggcgc gacgcgctgt gggtgccggg gacggatcac gccggcatcg
     1021 cgacgcagca cgtggtggag cgccggctgt ggaacgagga aaagaaagac cggcgcgacc
     1081 tgggacgcga ggcgtttctg gaacgggtct gggcgtggaa ggagcagtac ggcacgcgga
     1141 tcatcaacca gctgcggcgg ctgggctgct cgtgcgactg gtcgcggacg cggttcacga
     1201 tggacgaggg gctgtcgcgc gcggtgctga cggtgttcaa gcaactgtac gaagagggac
     1261 tgatctatcg cggcgactac ctggtcaact ggtcgccggc gctgcagacg gcgctgtcgg
     1321 acgacgaggt ggtgcacaag gaagtgcggg gccacctgtg gcatatccgt tatccgctgg
     1381 ccgacggttc ggggtcgatc gaggtggcga cgacgcggcc cgagacgatg ctcggcgaca
     1441 cggcggtggc ggtgcatccc gaggacgagc gctaccgcgg gatgatcggc aagcaggtgg
     1501 cgctgccgct gatggggcgg tcgatcccga tcatcgccga tccgctggtg gagcgggaat
     1561 tcgggacggg ggcggtcaag gtgacgccgg cgcacgatcc gaacgactac gagatgggcc
     1621 ggcggcacaa tctggaattc atcaacattc tgaatcccga cgggacgctc aacgccaacg
     1681 ccggggccta tgccgggctg gcggtggcgg aggcgcgcaa gcgggtcgtg gcggacctgg
     1741 aggcgcaggg tctgctggtc aaggttgagg atcatgttca cgcggtcgga cacgactatc
     1801 ggtcgggctg cgtggtggag ccctacctga gcaagcagtg gttcgtgcgg atgcggccgc
     1861 tgtgcgagcc ggcggtggag gcggtgcgcg cggggcgggt gcggttcgtg ccggagcatt
     1921 acgcgaacat ctactaccac tggatggaaa acgtgcgcga ctggtgcatc agccgccagc
     1981 tctggtgggg gcatcggatt ccgattttct actgcggcgc gtgcgagcac gtctggtgcg
     2041 agatcgagcg gacgccggcg cgctgcccgc ggtgcgatgc ggagcaggtg acgcaggatc
     2101 ccgatgtgct gaaccacgtg tccagcacat cgggatcctg cgtcacctgc tccgcat
//
LOCUS       DAOPCO010000442         2140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_40496_length_2140_cov_5.695444, whole genome shotgun sequence.
ACCESSION   DAOPCO010000442 DAOPCO010000000
VERSION     DAOPCO010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40496_length_2140_cov_5.695444"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLS90_17435"
     CDS             <1..1398
                     /locus_tag="PLS90_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04231.1"
                     /translation="ATAIPIGPWPAAAAVQGSFCASTKYSGSDNNSVGVLQSKPVTVE
                     VGDTALGYYLSTATAALNFDNLTVTLHDAADDSVLAGPQAVLQAGGIWYWQEFDLTGV
                     AAGTEVYVRFNDNDTLGFMVVDFVCVLGSGDFVAMVPETTPPPINYVTNGNLEDASPA
                     WTLTGGMQLKTDGAAIFGGAVSGSNYVTTFAGSPDGPAGSATSPDFTLPDEPQVILFN
                     AYSSGNTTKWALRRASDSAVLHEVVALAFGSPSWTTGALIGWPHMGELVNIYGNDPDT
                     PDYLMGDNFRTVPIRLLIDPFQSIGFLHPEAWDTSQAGDWDVTDAPTGAYPDPVPAHG
                     EGAASTRHDAVGNAGVGILQSQPFTVLAGDICVGYFISQATSSGNFDNLTVTLHDAAN
                     DSVIVGPPTPRQIGGVWYWQELSLAGVTPGTEVYVRFTDNDANGFMNIDFVCVLGTSG
                     PFTWVPTELSEFTLE"
     gene            1705..>2140
                     /locus_tag="PLS90_17440"
     CDS             1705..>2140
                     /locus_tag="PLS90_17440"
                     /inference="COORDINATES: protein motif:HMM:NF014766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HPK04232.1"
                     /translation="MKIQAIRTHNLCFEYPGRNGYLYAGGRVTGRVTTLVEVGTESGL
                     TGWGATYAHPDLARVIIEDHLAPHLIGRDAGAIESIWDLMYGLTRWYGRKGVAISALG
                     GIDIALWDLRGKREGRPLWQILGGERGQAPAYASALFWKARPE"
BASE COUNT          361 a          736 c          666 g          377 t
ORIGIN      
        1 gccactgcca ttccgatcgg cccctggccg gccgccgcgg ccgtgcaggg gtcgttctgt
       61 gcatcgacca aatactccgg cagcgacaac aacagcgtcg gcgtcctcca gagcaaaccg
      121 gtcacggtcg aggtcggcga caccgcgctc ggctactatc tctcgacggc aaccgccgcc
      181 ctcaacttcg acaacctgac cgtcacgttg cacgatgccg ccgatgactc tgtcctggcc
      241 ggaccgcagg ccgttctcca ggccggcggc atctggtact ggcaggagtt cgacctgacc
      301 ggcgtcgcgg ccggcaccga ggtttacgtc cgcttcaacg ataacgatac gcttgggttc
      361 atggtggtcg atttcgtctg cgtgctgggc agcggggatt ttgtcgcgat ggtccccgag
      421 acgacgcccc cgccgatcaa ctatgtgacc aacgggaacc tggaggacgc gtcgccggcg
      481 tggacgctga ccggcggcat gcagctcaag accgacgggg cggcgatttt cggcggcgcc
      541 gtcagcggca gcaattatgt gacgacgttc gccggctccc ccgacgggcc ggccggctcc
      601 gcgacgtcgc ccgatttcac actgcccgat gaaccgcagg tcatcctgtt caacgcctat
      661 tcgtcaggca acaccaccaa gtgggccctg cgccgcgcca gcgatagcgc ggtcttgcac
      721 gaggtcgtcg cgctggcctt tggcagcccc agttggacga cgggcgcgct gatcggctgg
      781 cctcacatgg gagaactggt caatatctac gggaacgacc cggacacccc ggactacctg
      841 atgggcgaca acttccgtac ggttcccatc cgcttgctga tcgatccgtt tcaatcgatc
      901 ggattcctgc atcccgaagc atgggatact tcgcaggccg gcgactggga cgtgaccgac
      961 gcgccgaccg gggcctatcc cgatccggtg ccggctcatg gcgaaggagc ggcttccacg
     1021 cgccacgacg ccgtcggcaa cgccggtgtc ggtattctgc aaagtcagcc gttcaccgtg
     1081 ctggcgggcg acatctgtgt cggctacttc atctcgcagg ccacctcgag cggcaacttc
     1141 gacaacctga ccgtgacgct gcacgacgct gcgaatgact cggtgatcgt cgggccgccg
     1201 acgccgcgac aaatcggcgg ggtctggtac tggcaggagt tgagtctggc cggcgtcacc
     1261 ccgggaaccg aggtttacgt ccgctttacc gacaatgacg ccaacggctt catgaacatc
     1321 gacttcgtct gcgtgctcgg aacgagcgga ccgttcacct gggtgccgac cgaattgtcg
     1381 gagttcacgc tggagtaacc ccccagcatg ttgtggccct gatgcgaagg gcagaggggt
     1441 aattcctctg cccttcccgt cggctgtaga attcgtctct tcatctcgcg ttctcgtgat
     1501 cacccccaat gacccgcccc caatgcttct ccctcgctct gacccgcggg ttcatgagca
     1561 actccgattc ggccgttctt cgggggagac tggccggggc ttggcctgcg gcggcagcgg
     1621 gcgcgcagct cgcgcgccgc tggaccggag cgtaacccgg aagcgcttgc ggcgcgaccc
     1681 aaaatcatgc atcatcgata aaccatgaaa atccaggcca tccgcaccca caacctctgc
     1741 ttcgaatacc ccggccgcaa cggctacctc tacgccggcg gccgggtcac cggccgcgtc
     1801 accacgctgg tcgaagtcgg cactgaatcc ggcttgaccg gctggggcgc cacctacgcc
     1861 catcccgatc tggcccgcgt gatcatcgag gatcatctgg cgccccacct gatcggccgc
     1921 gacgccggcg ccatcgaatc gatctgggat ctgatgtatg gcctgacgcg ctggtacggc
     1981 cgcaagggcg tggcgatctc ggccctgggg ggcatcgaca tcgccctctg ggatctccgc
     2041 ggcaaacggg aaggccgccc gctgtggcaa attctcggcg gcgaacgggg ccaggcgccg
     2101 gcctacgcca gcgccctttt ctggaaggcg cggcccgagg
//
LOCUS       DAOPCO010000443         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_41304_length_2109_cov_4.680623, whole genome shotgun sequence.
ACCESSION   DAOPCO010000443 DAOPCO010000000
VERSION     DAOPCO010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41304_length_2109_cov_4.680623"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PLS90_17445"
     CDS             complement(<1..115)
                     /locus_tag="PLS90_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPK04233.1"
                     /translation="MSILVDKSTRLVVQGMTGNEGKFHTAQMIDYGTQVVAG"
     gene            complement(201..1346)
                     /gene="sucC"
                     /locus_tag="PLS90_17450"
     CDS             complement(201..1346)
                     /gene="sucC"
                     /locus_tag="PLS90_17450"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020008663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPK04234.1"
                     /translation="MKIHEYQAKQIFRELGIPVPEGKVAETPDQAVEIARSLGTYPVA
                     VKAQVHVGGRGKAGGVKLAKSAEEAGQHARAILGMSIKGITVKRVLVEAGVDIQREIY
                     AGVIVDRERKAPVLILSAAGGVDIEEVAAADPEKILHLVLQPGLGLQAFQVRRACYFL
                     ELPKEAHRAMGETLHALERALFELDASLVEINPLVLTSRNEIIAADGKINLDDNALPL
                     HPDLEGLRDENEEEPLEAEARRHALSYVKLDGKIGCLVNGAGLAMGTMDLVKHFGGEP
                     ANFLDVGGGASPERIAEALRIVTSDASVNTILFNIFGGIVRCDRVAEGVLKARELTGI
                     DVPIVMRLVGTNEDKARELLEGTDLIMLPTMAEAAQKAVELSHRSGD"
     gene            complement(1835..2044)
                     /locus_tag="PLS90_17455"
                     /pseudo
     CDS             complement(1835..2044)
                     /locus_tag="PLS90_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
BASE COUNT          302 a          829 c          533 g          445 t
ORIGIN      
        1 cccccgccac cacctgcgtg ccgtaatcga tcatttgcgc ggtgtggaat tttccctcgt
       61 tgcccgtcat cccctgcacc accaggcggg tcgacttgtc taccagaata ctcatcgctt
      121 gactcgattc tctcgttctc gctgaatttc gtccctcgtc ctcgtcttcg cccccaacgg
      181 ccttcccaaa cttcggcccc ctaatcgccg ctccggtggc tcagttccac cgccttctgc
      241 gccgcttcgg ccatcgtcgg cagcatgatc aggtccgtcc cttccagcag ttcgcgcgcc
      301 ttgtcctcat tcgtgccgac caggcgcatc acgatcggga cgtcgatccc cgtcagttcg
      361 cgcgccttga gcaccccctc ggccacccgg tcgcagcgca caatcccccc gaaaatgttg
      421 aacagaatcg tgttgaccga ggcgtccgac gtcacgatcc gcagcgcctc ggcgatgcgc
      481 tcgggcgagg ccccgccccc cacgtccaga aagttcgccg gctccccccc gaaatgcttc
      541 accagatcca tcgtccccat cgccagtccc gccccgttga ccaggcagcc gatcttcccg
      601 tccagcttca cgtaactcag cgcgtggcgc cgcgcctcgg cctccagcgg ctcctcttcg
      661 ttctcgtcgc gcagcccctc caggtccgga tgcagcggca gcgcgttgtc gtccagattg
      721 attttcccgt cggccgcgat gatctcgttg cggctggtca gcaccagcgg gttgatctcc
      781 accagcgagg catccagctc gaacagcgcc cgctccagcg catgcagcgt ctcccccatc
      841 gcccggtgcg cttccttcgg aagctccaga aaataacacg cccgccgcac ctgaaacgcc
      901 tgcaggccca gtcccggctg caataccagg tgcaggatct tctccggatc ggccgccgcc
      961 acctcctcga tgtccacgcc ccccgccgcc gacaggatca gcaccggcgc cttgcgctcg
     1021 cgatccacga tcaccccggc gtagatctcg cgctggatgt cgacccccgc ctccaccagc
     1081 acccgcttga cggtgatccc cttaatgctc atccccagga tcgcgcgcgc gtgctggccc
     1141 gcttcttccg cgctcttggc cagcttcacg ccgccggcct tgccccggcc ccccacgtgc
     1201 acctgcgcct tgaccgcgac ggggtacgtc cccaggctcc tggcgatctc gaccgcctgg
     1261 tccggcgtct ccgccacttt cccttccggc accgggatgc ccagctcccg gaagatctgc
     1321 ttggcctgat actcatggat tttcataatc gtccgctcga aggccccggc ggggcggttt
     1381 gtctctggtc tggattgcgc aagccgtgca acgcatggtt ttacgccgcg attctactgg
     1441 cgcgattcgg gatgtcaaga catgccaacc ccccgcgtcc gttcacccca atcccgcccc
     1501 ttcgtcgatt ccatcgggct tatcctttac caggctctca ctccgctcgt ccgtcccttt
     1561 tgtcactgaa gtcccttatg tccctgaagt cccttacgtc ccttctgtcc ttccacgccc
     1621 cccagaacaa aaaaccgccc ggggacccaa cccccgggcg gcatcatcac caacttgaaa
     1681 atgatcagtc cttcgccgaa ccggtcgacg ccgaacttga cgccctcttc ggctcgctct
     1741 tcgccgcgct tcccgcggcc gctgtttcgc tcttgctctc gctcttgctc tccgatttgc
     1801 cgtcggcttt ggtttccgac ttgccttcgc cggttcccgc cttgccgttt gcgccgttat
     1861 tcttgtaatc ggtgatgtag aacccggagc ccttgaagat cagccccgcg cccgcgccga
     1921 tcagccgctt gaccgaccct tcggcgctgc acacctgaca cgtcgtcagc ggctcgtcct
     1981 tcatcgactg gaacgcctcg aaggtcgcgc cgcactggtt gcattcatac tcgtatgtcg
     2041 gcatggtgag gtgccctcgc tgccggggat gctcaggtcg ggggatgaat tcccgcgccc
     2101 gctggagcc
//
LOCUS       DAOPCO010000444         2070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_42336_length_2070_cov_4.886352, whole genome shotgun sequence.
ACCESSION   DAOPCO010000444 DAOPCO010000000
VERSION     DAOPCO010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42336_length_2070_cov_4.886352"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PLS90_17460"
     CDS             complement(<1..1064)
                     /locus_tag="PLS90_17460"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPK04235.1"
                     /translation="MAFVPLFCQSHHSPRGVASPADLVRRARALGYTTLGLCDEATLA
                     GFHAFDEACRAQGIRPVFGCRLMMEGLALAGMAFPLDLLIETEQGYRNLVRLMTVYHR
                     RGEGDRRPLRAEDLHDRVQGLFPILPPDGELLELVRLRERDKAELFLTRAGDLFGREL
                     VYGFSLQSEELVEAAGMTLPLARFVGARAIAAPQVRYPEPGDAAAAVYLDHPYGAPSR
                     SYRPPSDPKSLPALWGEDDLLSRWTDEYEELPHEAGALARRCTWRPGRIRRAYPTLDL
                     ERGFDPNSYLFDLVIRGATQRYGEITESLKQRVNRELEDVKANNLAPYLLLNHQIVQF
                     LDEKGISRGVGRSRAVSSVLA"
     gene            complement(1101..1583)
                     /locus_tag="PLS90_17465"
     CDS             complement(1101..1583)
                     /locus_tag="PLS90_17465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPK04236.1"
                     /translation="MTHPAFDIRPAQTGDLTAILELWSAMMRDHERSDERIRLAPGAV
                     AAYRTYANYHVSHPDSQVLVAEADSEIVGFVLLTISRNLPMFQPAHYGYLSDMAVRED
                     WRRRGVGQALLDRVRRWLMARDIHTIQLQYYDFNELGAAFWKAMGFRPYYTRMWLDLD
                     "
     gene            complement(1733..>2070)
                     /locus_tag="PLS90_17470"
     CDS             complement(1733..>2070)
                     /locus_tag="PLS90_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009753291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPK04237.1"
                     /translation="LPATEFPELAAVIERATLFVGGDTGPTHLAAALAKPTVAMMGPL
                     DARRNGPYGPGSVTIQHAVPRRAPWWANHRKWCDPRTDLRRVTVDEVLAACRAMLDRY
                     TGPTAGASP"
BASE COUNT          352 a          655 c          759 g          304 t
ORIGIN      
        1 gccaggacgg aggaaacggc gcgcgaacgc ccgacgccgc gcgaaatccc cttttcatcg
       61 aggaactgca cgatctggtg gttgagcagc agataggggg cgaggttgtt tgccttgacg
      121 tcctccagtt cgcgattgac gcgctgctta agggactcgg tgatctcgcc atagcgttgc
      181 gtggcgccgc ggatgaccag gtcgaacaga taggaattgg ggtcgaagcc gcgttccagg
      241 tcgagcgtcg gataagcccg gcggatccgt cccggacgcc aggtgcagcg ccgggcgagc
      301 gccccggcct cgtggggaag ctcctcatac tcatcggtcc agcggctcag cagatcatcc
      361 tcgccccaca gcgcggggag gcttttcggg tcactgggcg gtcgatagga gcgagacggc
      421 gcgccgtacg gatgatcgag atagacggcg gcggcggcgt cgcccggttc aggatagcgg
      481 acctgcgggg cggcgatggc gcgcgcgccg acgaaccgcg ccagcggcag cgtcattccg
      541 gccgcctcga ccagctcctc ggattgcaga gaaaaaccgt agaccaattc ccggccaaag
      601 aggtcccccg cccgcgtgag aaacagctcg gccttgtcgc gttcgcgcag gcggaccagt
      661 tcgagcaact cgccatcggg ggggaggatg gggaagagcc cctggacacg gtcgtgcagg
      721 tcctcggcgc gcagcggccg gcgatcgcct tcgccgcgcc ggtggtagac cgtcatcagc
      781 cgcaccagat tgcggtagcc ctgttcggtt tcgatcagca gatcgagcgg aaaggccatg
      841 ccggcgaggg cgagcccctc catcatcagg cggcagccga agacggggcg aatcccctgg
      901 gcgcggcagg cctcgtcaaa ggcatggaag ccggccagcg tggcctcgtc gcacaggccg
      961 agcgtggtat agcccaaggc gcgcgcgcgg cgcaccaggt cggccggtga ggcgacgccg
     1021 cgcggactgt ggtgggactg gcagaagagc ggaacgaagg ccatcggcgc cggcaggaat
     1081 cctcccctga agtaagccgc tcagtcgaga tcgagccaca tccgcgtgta ataagggcgg
     1141 aagcccatcg ctttccagaa ggcggcgccc agctcgttga agtcgtagta ttgaagctgg
     1201 atggtgtgga tatcgcgcgc catgagccag cggcggacgc gatcgagcaa ggcctggccg
     1261 acgccgcgcc gccgccagtc ctcgcgcacg gccatatccg acaggtagcc gtaatgggcc
     1321 ggctgaaaca tcggcagatt gcggctgatc gtcagcagga cgaagccgac gatctcgctg
     1381 tccgcttcgg ccaccagcac ctgggaatcg gggtggctga cgtgatagtt cgcgtaggtg
     1441 cgataggcgg cgacggcgcc cggggccagc cggatgcgct cgtcgctgcg ttcgtggtcg
     1501 cgcatcatgg cggaccacag ttcgaggatc gcggtgaggt cgccggtctg ggccgggcgg
     1561 atgtcaaacg ctgggtgggt catgggtggg agcggagccc catcgtggaa gtcgccttgc
     1621 cggcggatcg cgtcacgccg cgcacgtatg atgcaggaaa agccggcgaa tcgccagccg
     1681 tcccgtcgct gccggggtgt cgactcgatt cctcgctcat cgagccgaag gatcatgggg
     1741 atgcgccggc cgttgggccg gtgtaccggt ccagcatggc gcggcaggcc gccaggacct
     1801 cgtcgaccgt gacgcggcgc aggtcggtgc gcgggtcgca ccacttgcga tgattcgccc
     1861 accacggcgc gcggcgcgga acggcgtgct gaatcgtgac gctgccgggg ccgtagggcc
     1921 cgttgcggcg cgcatcgagc ggccccatca tcgccacggt cggcttcgcg agggccgcgg
     1981 ccagatgcgt cgggccggtg tcgccaccga caaacagggt cgcccgttcg atcacggcgg
     2041 ccagctcggg gaactccgtc gcgggcaacg
//
LOCUS       DAOPCO010000445         2066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_42451_length_2066_cov_4.357036, whole genome shotgun sequence.
ACCESSION   DAOPCO010000445 DAOPCO010000000
VERSION     DAOPCO010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42451_length_2066_cov_4.357036"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLS90_17475"
     CDS             <1..594
                     /locus_tag="PLS90_17475"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPK04238.1"
                     /translation="GITGQDGGYLAEQLLAEGVAAEKILNEALIPGMREVGRKFQNNE
                     AFVPDMLISADAMKKAMALLKTELANSNVEPKGTFIVGTVQGDMHDIGKNLVAMMVEG
                     AGWEVIDLGVDVSADKFLNAVEQHPKAAVGMSALLTTTMGAMKTTVEKIRAKFPNTTI
                     IIGGAPVTDSFAKEIKASGYAPDPQGAIELLEGIAVA"
     gene            complement(876..1796)
                     /locus_tag="PLS90_17480"
     CDS             complement(876..1796)
                     /locus_tag="PLS90_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04239.1"
                     /translation="MPPYSKAPVYLFYGNQVDAVLRARDQVLDAVLPAELRAENLTEY
                     FPSGAGDTVRFGELLDEIAGDMAMLSFIPEAPKCVVVTNPAELFSGAARGRARKTDKP
                     DSAERALEHAVRWIERDLPQTGHTLMFIALEDEAAAREVNERSPLFQAISRNGVARRF
                     SEKKAFFRIEEAILSRNANDLIQATRDLWKPGKGDQAVYGGVVRTLRYLIQANVARER
                     KLADTPAELALLFPDEAQRNLFKANPSVTRKYLGRAVYRTAHLLEAYQGMLDVYRALR
                     PRPDDLYVADAQGLMEQVLVRLLNSPRPAR"
BASE COUNT          385 a          558 c          791 g          332 t
ORIGIN      
        1 gggattaccg gccaggacgg tgggtacctc gccgagcaac tgctggccga gggcgtggcc
       61 gccgagaaga tcctcaacga agcgctgatc ccggggatgc gcgaggtcgg ccgcaagttt
      121 cagaacaacg aggcgttcgt gccggacatg ctgatctcgg ccgacgcgat gaagaaggcg
      181 atggcgctgc tcaagacgga gctggccaac agcaacgtcg agccgaaggg gacgttcatc
      241 gtcgggacgg tgcagggcga catgcacgac attggcaaga acctggtggc gatgatggtc
      301 gagggagccg gctgggaagt gatcgacctg ggggtcgacg tttcggcgga caagttcctc
      361 aatgccgtcg agcagcaccc gaaggcggcg gtcgggatgt cggcactgct gacgacgacg
      421 atgggggcga tgaagacgac ggtcgagaag atccgcgcga agttccccaa cacgacgatc
      481 atcattggcg gcgcgccggt gaccgacagt ttcgccaagg agatcaaggc cagcggttac
      541 gctcccgatc cgcaaggcgc gattgaattg ctcgagggga tcgccgtcgc gtagggcggt
      601 attggttcgg tccgaattga ggccccgcgc cccgcagcag gcacacgctg cggtggtgcg
      661 gggcctgttt gtgtttttcg aaaggctaat aagggccata agggacggat ccaaagcggc
      721 gccgacgcgt tgcgctgcaa tgctctggca ccggactccc aaagaattgc ctcaacctgc
      781 tgcctgagaa ggtcgaatgc ggggcggcgc gaagcgtggt gggatacacg cgccgtcaat
      841 gagcgattcg gcgacatccg aaatgcgtcc ggcggttaac gggccgggcg gggggagttg
      901 aggaggcgga ccaggacctg ctccatcagg ccctgggcgt cggcgacgta gaggtcgtcg
      961 ggccgggggc gcagcgcgcg gtagacgtcg agcatgccct ggtaggcttc gagcagatgc
     1021 gccgtgcggt agacggcgcg gccgaggtat ttgcgcgtga cggaggggtt ggccttgaac
     1081 agattgcgct gggcctcgtc ggggaagagc agggcgagtt ccgcgggcgt gtcggcgagt
     1141 ttgcgttcgc gcgcgacgtt ggcctggatc agatagcgca gcgtgcggac gacgccgccg
     1201 tagaccgcct ggtcgccctt gcccggtttc cagagatcgc gcgtggcctg gatcaggtcg
     1261 ttggcgttgc ggctgaggat tgcctcttcg atgcggaaga aggctttttt ctcgctgaag
     1321 cggcgggcca cgccgttgcg gctgatcgcc tggaacagag gggagcgctc attgacctcg
     1381 cgggcggccg cttcatcctc gagcgcaatg aacatcagcg tatggccggt ctggggcaga
     1441 tcgcgttcga tccagcgcac ggcatgttcg agggcgcgct cggcggaatc cggcttgtcg
     1501 gtctttctgg cgcggccgcg ggcggcgccg ctgaagagct cggcgggatt ggttacgacg
     1561 acgcacttgg gggcctcggg gatgaacgac agcatcgcca tgtcgccggc gatctcgtcg
     1621 agcagttcgc cgaagcggac ggtgtcgccc gcgccgctgg ggaaatattc ggtgaggttt
     1681 tcggcgcgca gttcggcggg caggacggca tcgagcacct ggtcgcgcgc gcgcaagacg
     1741 gcgtcgacct gattgccgta gaagagatag accggagcct tggagtaagg cggcatggtg
     1801 gagcctccac cccagcatct tttcggcagg ggcagcgaga ttcaatgaaa taacgcgcgc
     1861 gggcaagacg gggatggaag ggacatcagg gacgcaaggg acgtcaggga cagaaataaa
     1921 ggacagaaga aaaagtcaga cggaagcggg ccgccggggt cagagtgcct gtttttcggt
     1981 tagtggttcg ggaatggggg aggacgcgcc ggacccggct cgccggcggc ggaggaagag
     2041 gagcaggcag aggccggcga ggccgg
//
LOCUS       DAOPCO010000446         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_42811_length_2053_cov_4.407908, whole genome shotgun sequence.
ACCESSION   DAOPCO010000446 DAOPCO010000000
VERSION     DAOPCO010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42811_length_2053_cov_4.407908"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLS90_17485"
     CDS             <1..215
                     /locus_tag="PLS90_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04240.1"
                     /translation="WVVAFFRDAKDRDPIIVRQDHPGDGQWHTLEIDTRVPTDTLIRY
                     FKFTLNLRAQASQPAFVDSVCARLSK"
     gene            366..>2053
                     /locus_tag="PLS90_17490"
     CDS             366..>2053
                     /locus_tag="PLS90_17490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPK04241.1"
                     /translation="MIAHLRSQGYRVGLIAIEHPRSARRELKAQVDRLWIVKSQPDLP
                     VRERLRLAIRGAFRRLLPQPLYLLGKRVLYQFIEHPRPAPPAPRPTSFILRKINPRMR
                     AITARVVKEFWPRAVIAQYVWSAHALDVVPDQILTVIDTIDVQHRRREHALAAGSDLP
                     HHRCSREEETRELQRADVLIAIQRHETHDLAGMCPQARVICVGHAVDRLERLPSPEDG
                     ARRILFVGARYDPNIQGMRLFMDEAWPRIRAAVPDAELVICGRVCDDLHQLPPGVRLE
                     GLVPDLTARYAQAAVVINPVPYGTGLKIKTVEALSHGKCFVGTQAAVIGLEDLEPRPY
                     IVTADMEEMGDVVARLLLNPAERRAMEERAWQAARDRLAPQQVYRPLMEILEQAKTIP
                     RVRRRGEHGVPGDGNAGLPDQIRNGSFEQWAPGGPVAWTVTPEGHQALRGRTPDGAAA
                     VELPPAPDRQATLFQLVRTNGVAAGAAFEIEMEALASEPGKFGLVVTLFPRSGGTPVI
                     HASNHAGGGQWQRLALSIRLDNPSAVQQIKVVANLRAGATRPVWIRHCASRWEAA"
BASE COUNT          342 a          684 c          680 g          347 t
ORIGIN      
        1 gctgggtggt cgcctttttt cgcgacgcga aggaccgtga cccaatcatt gtgcgccagg
       61 atcaccccgg cgacggccaa tggcataccc tcgagattga cacccgcgtg ccgaccgaca
      121 cgctcatccg ctatttcaag ttcactttga atctgcgggc ccaggccagc caaccggctt
      181 tcgtcgatag cgtctgcgct cgcctcagca agtagatatc cagtgtcgcc cgctcacgag
      241 gcgaggcgtc atgatgggtt gttggaacga tggtcggtaa cagcgcaaac gtaccgtgga
      301 ttgtggtcgt aaccagtgat ccccttgtgc cggtgacggg aggcggcgcg cgccggatgc
      361 tgcgcatgat cgctcacctg cgctcgcagg gctatcgggt cgggctgatt gccatcgagc
      421 accctcgatc ggcgcggcgg gaactcaaag cgcaagtgga ccggctgtgg atagtcaaat
      481 cgcagcctga tctgccggtg cgggagcggc tccgcctggc aatcaggggg gcgttccgtc
      541 ggctgttgcc tcagcccctc tatctgctcg gcaagcgcgt tttatatcag tttatcgaac
      601 atccccggcc ggcccccccc gcgccgcgtc ccacctcctt cattctgcga aaaatcaacc
      661 cgcgcatgcg tgcgatcacc gcgagggtcg tcaaggaatt ctggccccgg gccgtgattg
      721 cacagtatgt ctggagcgcc cacgcgcttg acgtcgtccc ggatcagatt cttacggtga
      781 tcgacaccat tgatgttcag catcggcgtc gcgagcacgc cctggccgcc ggcagcgacc
      841 tgccgcatca ccggtgctcg cgcgaagaag aaacccgtga gctgcaacgc gccgatgtcc
      901 tgatcgccat acagcgccat gagacccatg acctggccgg gatgtgcccc caggcgcgcg
      961 tgatctgtgt cggccacgca gtggatcgtc tcgagcgcct cccctcgccg gaggatggcg
     1021 cgcgacggat tctgttcgtc ggagcgcgct acgatccgaa cattcagggg atgcgcctct
     1081 ttatggacga agcctggccg cgcatccgcg ccgccgtgcc cgatgcggaa cttgtgatct
     1141 gcggacgcgt gtgcgacgat ctgcatcagc tccctccggg cgtccggctt gaagggctcg
     1201 ttcccgatct gaccgcgcgg tatgctcagg ccgcggtcgt catcaatccc gtgccctatg
     1261 gaaccgggct gaagatcaag accgtggaag ccttgagcca cggcaaatgc tttgtcggca
     1321 cccaggcggc cgtcatcggg ctggaggact tggagccccg cccctacatc gtcacggccg
     1381 acatggagga gatgggcgac gttgtcgcgc ggctgttgct caacccggcc gagcggcgcg
     1441 ccatggagga gcgggcatgg caggcggccc gcgaccggct tgcgccgcaa caggtctacc
     1501 gcccgctgat ggaaatcctc gagcaggcga agacgatccc gcgggtccgc cgccggggcg
     1561 agcacggggt tccgggggat ggcaacgcgg gattgcccga tcagattcgc aacggctcgt
     1621 tcgagcaatg ggcgcccggc ggcccggttg cctggactgt gacgccggag ggacatcagg
     1681 cgctgcgggg gcggacgccc gacggcgccg ccgccgtcga gctgcccccc gcgcccgacc
     1741 ggcaggcaac cctgttccaa ctcgtgcgaa ccaacggcgt cgccgccggc gccgcgttcg
     1801 agattgaaat ggaggcgctg gcgagcgagc cgggcaagtt cgggttggtg gtcacgctct
     1861 tcccccggag cggcgggacg ccggtcatcc acgcatcgaa tcacgccgga ggtgggcaat
     1921 ggcaacgact ggcactgagc atccggctgg acaacccatc cgccgtccaa cagatcaagg
     1981 tggtggccaa cctgcgggcc ggcgccaccc ggccggtctg gatccggcac tgcgccagcc
     2041 gctgggaagc ggc
//
LOCUS       DAOPCO010000447         1993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_44608_length_1993_cov_3.021672, whole genome shotgun sequence.
ACCESSION   DAOPCO010000447 DAOPCO010000000
VERSION     DAOPCO010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44608_length_1993_cov_3.021672"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..437)
                     /locus_tag="PLS90_17495"
     CDS             complement(114..437)
                     /locus_tag="PLS90_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HPK04242.1"
                     /translation="MINVTEAAVREMKRLLKDRDLNGYGLRVGVKGGGCSGLTYVMDF
                     EAKPTDHDRVFEADGVSIFCDPKSYLYLNGLTLDYSNELMGGGFRFSNPNAQRTCSCG
                     TSFSA"
     gene            complement(630..1817)
                     /locus_tag="PLS90_17500"
     CDS             complement(630..1817)
                     /locus_tag="PLS90_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPK04243.1"
                     /translation="MGLQLPVYMDAHATTPCDPAVIDAMLPTLGRMFGNAASSHHAFG
                     WEAEALVEQARELLAGLIGATPSEIIFTSGATESDNLAIKGVAHAYRRKGRHLITSQI
                     EHHAVLHSFQALEREGFEVTYLPVDRSGRVDPEDLRRAIRNETILVSVMAANNEIGVI
                     QPLAEIGAICAENGIFFHTDAVQALAHVKIDVNTSAVDLLSVSAHKMYGPKGVGALYI
                     RRRNPRVRLEPLLSGGGHERGIRPGTLNVTGIVGLGKAAELVVAARDEESRRIARLRD
                     RLQQRLLDQLDGVRVNGGEDHRLPNNLHVTFQGVRAEWMLKAMRNVAVSSGAACASAS
                     NEPSHVLRALGATPEDAESSLRFGLHRFTTEQEVDFAADLTVKTVQWLRQNTPDANLE
                     YCK"
BASE COUNT          374 a          569 c          711 g          339 t
ORIGIN      
        1 gtcatcggcg ggaagccctt ccatcggcgc gcggccgggg gtggatgcca caggaacaag
       61 aaacccccgc cgggaccgga atcaggcccg cggcggggga gctgggatac gggtcaggca
      121 ctgaacgacg tgccgcagga acaggtgcgc tgggcgttgg ggttgctgaa acggaagccg
      181 ccgcccatca attcgttgct gtaatcgagc gtcaggccgt tcagatagag gtagctcttg
      241 gggtcgcaga agatggagac gccgtcggcc tcgaagacgc ggtcatggtc ggtcggtttg
      301 gcttcgaagt ccatgacata ggtcaggccg gagcaaccgc cgcccttgac gccgacgcgc
      361 agaccgtagc cgttcaggtc gcggtccttc agcaagcgtt tcatttcgcg gacggccgcc
      421 tcggttacat taatcatcgt tgaagatcct cccctaagcg ggtgtcagcg ggttggtcca
      481 cgtgctcaag cagagcgaga aggatgccgt ggtgtccgga tgcaggaagg gatcgccaca
      541 gatgggacga ccccttcgac actccgtttg atcgtagcag aacggggcag aagcgtcaag
      601 ggacgccccc aggatttcat gtaacctatt tatttacagt attcaagatt ggcgtcgggg
      661 gtgttctggc gaagccactg gacggttttg acggtcagat ccgcagcgaa atccacttcc
      721 tgttcggtgg tgaaccggtg caggccaaaa cgaagcgagg attcggcgtc ctcgggcgtg
      781 gcgcccaacg cgcgcaggac gtgggagggt tcgttggagg ccgaggcaca ggcggcgccc
      841 gaactgaccg cgacgttgcg catggccttg agcatccact cagctctgac gccctggaac
      901 gtcacatgca ggttgttggg gaggcggtgg tcttcgccgc cattgacgcg gacgccgtcg
      961 agctggtcga ggaggcgctg ctgcaaccgg tcgcggaggc gcgcgatgcg ccggctctcc
     1021 tcgtcgcgcg cggcgacgac cagttcggcg gccttgccca ggccgacgat gccggtgaca
     1081 ttcagcgtgc cggggcgaat gccgcgctcg tggccgccgc cgctgaggag gggttccagc
     1141 cggacgcggg ggttgcggcg gcggatatag agggcgccga cgcccttggg gccatacatc
     1201 ttgtgggccg aaaccgagag cagatcgacc gcggaggtat tgacgtcgat cttgacgtgg
     1261 gcgagggcct gaacggcgtc ggtatgaaag aaaatcccgt tttcggcaca gatcgcgccg
     1321 atttcggcca gcggctggat gacgccgatt tcattgttgg ccgccatgac cgagaccagg
     1381 atcgtttcat tgcggatggc gcggcgcaag tcctccggat cgacgcggcc ggaccggtcg
     1441 acggggaggt aggtgacctc gaacccctcg cgttcgagcg cctgaaacga atgcaggacg
     1501 gcgtgatgct cgatctggct ggtgatcagg tggcggccct tgcggcggta ggcgtgggcg
     1561 acgcccttga ttgccagatt gtcggactcg gtcgcgccgg aggtgaagat gatttcggaa
     1621 ggggtggcgc cgatcaggcc ggcgaggagc tcgcgcgcct gttcgaccag cgcctcggct
     1681 tcccagccga aggcgtggtg gctactggcg gcgttgccga acatgcgccc gagcgtgggc
     1741 agcatggcgt cgatgacggc gggatcgcat ggcgtcgtcg catgcgcatc catgtagaca
     1801 ggcaattgga gtcccatacg acttcccggg gaaaacaaca ataaccggcc acggccggag
     1861 gcgcaccccg tgtgagcttg cggagcagga accatacccc gaccgccagc gctactgtca
     1921 gaataagact aacacaggca aaatcaacag gagacgccaa atcgggggtc gatgcggatg
     1981 gagcaagcgg ggc
//
LOCUS       DAOPCO010000448         1803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_51600_length_1803_cov_2.794622, whole genome shotgun sequence.
ACCESSION   DAOPCO010000448 DAOPCO010000000
VERSION     DAOPCO010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51600_length_1803_cov_2.794622"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..445)
                     /locus_tag="PLS90_17505"
     CDS             complement(161..445)
                     /locus_tag="PLS90_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04244.1"
                     /translation="MALWDGRPPDAVAPADAVAVVHAFLTRCRRWALEQELPRRHREV
                     DQTWDAAAAARLHAWCAYLEFTEHALRELEAGALDDWFGEAQPEPPAEQR"
     gene            complement(448..747)
                     /locus_tag="PLS90_17510"
     CDS             complement(448..747)
                     /locus_tag="PLS90_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04245.1"
                     /translation="MADEDPQSGTQRDLIGHVWVESGRLLLTDPIYREEFDEETLARA
                     GDRAQRAGLLNDGMAALFQTGIRIGRYPVYAHRFANGALARVEIDLQASAPEAEG"
     gene            complement(1000..1521)
                     /locus_tag="PLS90_17515"
     CDS             complement(1000..1521)
                     /locus_tag="PLS90_17515"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPK04246.1"
                     /translation="MNQELIDAVRAGDPEKVRECLHAGADPNLKDRKGEDVLSIAIDD
                     SPESVEMVRVLLDAGADPNCQWRDCVPLTCAIIDKNLDVIELLLQRGANPNLVIDGGT
                     QYDFADFDYRYDEYDLRLPVEPTEAEMGNEEAWLAMLDRLALEYGKRPPEVLHLLRKY
                     GGRSLRELRDEPR"
BASE COUNT          305 a          626 c          563 g          309 t
ORIGIN      
        1 gccaggtcgg ccgcgcgccc gtaaaggcga tccccttgcg caggttctcc gccaggccgg
       61 cgacctgttc ccggatcgcg cctggcagtt caatcgcgat acaaccgcag ccggtccggc
      121 ccggtcggcg catcggcggc gggggggagg gcgtcggcgt tcatcgctgc tccgccggcg
      181 gctcgggctg cgcctcgccg aaccagtcat ccagcgcgcc cgcttccagt tcgcgcagcg
      241 cgtgctccgt gaattccagg taggcgcacc aggcgtgcag ccgggcggcg gcggcggcat
      301 cccacgtctg gtccacttcc cggtggcgcc gcggcagttc ctgctccagc gcccaccgcc
      361 ggcagcgcgt caggaaggcg tgcaccacgg cgaccgcatc ggccggcgcg acggcatcgg
      421 gcgggcgtcc gtcccacagc gccacgtcta gccctccgct tccggcgccg acgcctgcaa
      481 gtcgatttcg acgcgggcca gcgccccgtt ggcgaatcgg tgggcgtaga ccggatagcg
      541 cccgatccgg atcccagtct ggaacagcgc cgccatgccg tcgttgagca ggccggcgcg
      601 ctgcgcacgg tcccccgccc gcgccagcgt ctcctcgtcg aattcctcgc ggtaaatcgg
      661 atcggtcagc agcagccgtc ccgattcgac ccagacatga ccgatcagat cacgctgggt
      721 gccggactgc gggtcctcgt cggccatggt ccatgcaacc tcgcttgaaa atcgtccctc
      781 gtccccggct ttcgaaaaca tgctcgcgcc gcggggcccg cgagtccgcg ccaggggaaa
      841 ccgcttccgc cgccgctctc catggtacca gaaccaagcc gccgcgcaag cccgccgacc
      901 cagcgacgat gctcgtgtgg gagcggcccc cccgcgaagc cctacggcga gccgccgagg
      961 cgggcaaccg aattgctccc cattctctct gggcgggttt tacctcggtt cgtcccgcag
     1021 ttccctcagc gaccgtccgc catacttgcg cagcagatgc aggacctccg gcggacgctt
     1081 cccgtattcg agcgccaacc ggtccagcat cgcgagccat gcctcttcat ttcccatctc
     1141 tgcttccgtc ggttccacgg gcagtctcag atcatactca tcatatcgat aatcaaaatc
     1201 ggcgaaatcg tactgcgtcc ccccgtcgat cacgagattc gggttagccc cgcgctgcag
     1261 cagcaactcg atcacatcca ggttcttgtc gataatggcg caggtcaagg ggacgcaatc
     1321 acgccattgg caattcggat cggcgcccgc gtccagcagc actcgcacca tctccacgga
     1381 ttccggcgaa tcatcgatcg cgatggacaa cacgtcctca cccttccggt ctttcaaatt
     1441 gggatcggcg cccgcgtgca ggcactccct gactttctca ggatcccccg cccggacggc
     1501 atcgatcagc tcttgattca tcgttgatcc ctcccagccc cgattcaaac ggcgcctgtc
     1561 aagttccgta tcctgcccca gatattaaga agtgaaacct agtgtagtga ttgttgttca
     1621 aattttaaag tgaggttgca gggcaacagc aagcggccga atccggggtt gaattcggct
     1681 gggtgttgag gaggcgggaa gcgaggaaga cgcgatgaag ggagggggcg gatgccaatc
     1741 ggtgaggggc ggcggcgtga ggggcgcagg ggtgatcaag gagcgaggcg gctggaagga
     1801 ggg
//
LOCUS       DAOPCO010000449         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_52482_length_1782_cov_5.178923, whole genome shotgun sequence.
ACCESSION   DAOPCO010000449 DAOPCO010000000
VERSION     DAOPCO010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52482_length_1782_cov_5.178923"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLS90_17520"
     CDS             <1..1609
                     /locus_tag="PLS90_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPK04247.1"
                     /translation="SFWFAGTPPDYNSHGPTWENDPVQGRVGLITAQCAANFRNIAVE
                     PLSDFSSIVTPQRGRRDAEGNLLPRWSYAETIRKMGEWMIHADEIVDKSSVPEAFRDL
                     PPYVLSNFVRTDDVIWSLWGEAAFNHSLLITGCVAYYEFTGDDRILAVARKTADWHLE
                     NDTPDDWALPHLPPSVVEFKEDGTWQGQSWGLELDKSAYFGLALLRLYGVTGDARYLD
                     QAQRIADTLVGFQQEEGNWPFRVNAQTGEVQHGYACSQLWYVWFFNLLADVTGEDAHR
                     ERADRALQWLLENPVQTNQWIGLYGDVPSGAETYDQWVALETAMYLLEHRDRYPDALE
                     IAKKIEAFIEEKIMEPYGFHEGVPGVVEQSSYRVVLSHHQLRLAEFYATMYQATGDEN
                     YKQRAIETANSVTWIVMSDGKMRQGFWYHSQAAVLILSLVEQFMRVMACIPETAPDDE
                     SHLLRHQGLLNEIDYSTGTLRYRTTASAEEWLTLAGQPAAVQAGGVELPRAEAWPAAG
                     PGWWTAPDANAVRIRHDAPDVQINFAD"
BASE COUNT          325 a          694 c          484 g          279 t
ORIGIN      
        1 cagcttctgg ttcgccggca ccccccccga ttacaactcc catggtccca cctgggagaa
       61 tgatcccgtc cagggccgcg tcggcctgat caccgcccag tgcgccgcca acttccgcaa
      121 catcgccgtc gagccgctct ccgatttctc ctccatcgtc acccctcagc gcggccggcg
      181 cgacgccgag ggcaacctgc tcccgcgctg gtcatacgcc gaaaccatcc gcaagatggg
      241 cgaatggatg atccacgccg atgagatcgt cgacaaatcg agcgtccccg aagccttccg
      301 cgacctgccc ccctatgtcc tgagcaactt cgtccgcacc gacgacgtca tctggagcct
      361 ctggggcgaa gccgccttca accactcgct cctcatcacc ggctgcgttg cctactacga
      421 attcaccggc gacgaccgca tcctcgcggt cgcgcgcaag accgccgact ggcatctcga
      481 gaacgacacc cccgatgact gggcgctccc ccacctgccc cccagcgtcg tcgaattcaa
      541 ggaggatggc acctggcaag gccagtcctg gggcctcgag ctggacaagt ccgcgtattt
      601 cggcctcgcc ctgctgcgcc tctatggcgt caccggcgac gcccgctacc tcgaccaggc
      661 ccaacgcatc gccgacacgc tcgtcggctt ccagcaggag gagggcaact ggcccttccg
      721 cgtcaacgcc caaaccggcg aggtccagca cggctacgcg tgcagccagc tctggtatgt
      781 ctggttcttc aatcttctgg ccgacgtcac cggcgaggac gcccaccgcg agcgcgccga
      841 ccgcgccctg caatggctcc tcgaaaaccc cgtccagacc aaccagtgga tcggcctcta
      901 tggcgacgtc ccgagcggcg ccgaaaccta cgaccagtgg gtggcgctgg aaaccgccat
      961 gtacctcctc gaacaccgcg accggtatcc cgacgccctt gaaatcgcga aaaaaatcga
     1021 agccttcatc gaggaaaaaa tcatggaacc ttacggcttc catgagggcg tccccggcgt
     1081 cgtcgagcaa agctcctacc gcgtcgtcct ctcgcatcac cagcttcgcc tggccgagtt
     1141 ttacgccacg atgtaccagg ccaccggcga cgagaactac aagcagcggg ccatcgaaac
     1201 cgccaactcc gtcacctgga tcgtcatgtc cgacggcaag atgcgccagg gcttctggta
     1261 tcactcccag gccgccgtgt tgatcctgtc gctcgtcgag cagttcatgc gcgtcatggc
     1321 ctgcatcccg gaaaccgccc cggacgatga aagccatctg ctgcgccacc agggcctgct
     1381 caacgagatc gactattcga ccggcacgct gcgctaccgc accacggcgt cggccgagga
     1441 atggctcacc ctcgccggcc agcccgccgc ggtccaggcc ggcggcgtcg aactcccccg
     1501 cgccgaggcc tggcccgccg ccggccccgg ctggtggacc gcccccgacg caaacgccgt
     1561 ccgcatccgc cacgacgccc ccgacgtcca gatcaacttc gccgactgat ccaggaggac
     1621 ccggcttgcg cccgccttgc tgactcatgc tgtccagatt ccgttcgcgt cagccgcccc
     1681 agccataagc gaccgattcg aacgctcaaa gccttgccac actggacccg ccggcagtcc
     1741 cgccagggac tgccgacttc agcccgccac ttcagtggcg gg
//
LOCUS       DAOPCO010000450         1776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_52749_length_1776_cov_5.153980, whole genome shotgun sequence.
ACCESSION   DAOPCO010000450 DAOPCO010000000
VERSION     DAOPCO010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52749_length_1776_cov_5.153980"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1776
                     /locus_tag="PLS90_17525"
     CDS             114..>1776
                     /locus_tag="PLS90_17525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPK04248.1"
                     /translation="MTRDSRHPPEQAAERPAPFWRRPLLKLSPALVVLVLSLVATVYC
                     WRAIRQEVYAKTYAAFQEEARAARNRVEHRFDQISKALLGVQSLYAASQNVLDDELRD
                     YINRLGLVHAREEIQGLLLVRPVSSRDPGEWTADHLTEETLTGRLETRVLSADPPREE
                     GLDEAITRHPQFADTLIQSLARDTVICKPFNSADEIGDRDFYLAAVPLFQHQDSRSGE
                     FAGWICAWFRLDSLFASDDLASIPSLRFHLFKSSEVEGETPRVGLKPHLPVEVPRGQD
                     SFHFQETASLVFGGHNWVLQVGSRSDFDPLDLEAAPWAVLCTGLILSLLLYLLVYSLN
                     STRENAIAIARRMTTDLRRREEEASRLAMVVSRTDNGVVITDAQCRILWVNQSYERIT
                     GYQLAEIQGLRPSEFLHGPETDPLTVEMMRRHYQEGKPFKAEVLNYARNGRKFWVHVE
                     VQPVFDEAHHLTHFMGLTSDITERRLSEEALRESERRYRGLIESQHDIILRTDAHGLL
                     TYVNDVYCEFFDVTALDALGTPYSPEIHPDDQAKTREALQRLSMPP"
BASE COUNT          293 a          678 c          508 g          297 t
ORIGIN      
        1 gattccgagc gatggccccc gcatgcggag ccgcggagcg gttcgcttgc cggcccaggg
       61 ctggcctccg ccctccgcgc gcggccagcg cccgacctgg atggatcgac tgcgtgactc
      121 gtgactcgcg acaccctccg gagcaggctg ccgagcgtcc cgcccccttc tggcgccggc
      181 cgctgctcaa gctcagcccg gccctcgtcg tgctggtcct ttcgctcgtc gccaccgtgt
      241 actgctggcg cgcgatccgc caggaggtgt atgcaaaaac atacgccgcc ttccaggaag
      301 aggcgcgcgc cgcccgcaac cgcgttgagc accgctttga ccagatcagc aaggccctgc
      361 tcggcgtcca gagtctgtac gccgccagcc agaacgtcct cgacgacgag ctgcgcgatt
      421 acatcaaccg cctcggcctc gtccacgccc gcgaggagat tcagggcctg ctcctcgtcc
      481 ggccggtctc aagccgcgac cccggagaat ggaccgcgga ccacctgacc gaggagacgc
      541 tcaccgggcg cctcgagacc cgcgttcttt ccgccgatcc cccccgcgag gagggcctcg
      601 acgaggcgat cacccgccat ccgcagttcg ccgacacgct gatccagtcc ctcgcccgcg
      661 atacggtcat ctgcaagccg ttcaactccg ccgacgagat cggcgatcgc gatttctacc
      721 tggcggcggt cccgctgttc cagcatcagg acagccgcag cggcgaattc gccggctgga
      781 tctgcgcctg gttccggctc gacagtctct tcgccagcga cgatttggcc tcgatcccct
      841 cgctgcgctt ccatctgttt aagtcgagcg aggtcgaggg cgaaacgccc cgcgtcggct
      901 tgaagccgca tctgccggtc gaggtcccgc gcgggcagga ctccttccac tttcaggaaa
      961 ccgcgtcgct cgtcttcggc ggccacaact gggtgctgca ggtcggttcg cgctccgatt
     1021 tcgatcccct cgatctggag gccgccccgt gggccgtcct ctgcaccggc ctgatcctta
     1081 gcctgctgct ctacctgctc gtttattcgc tcaattcaac gcgcgagaac gccatcgcca
     1141 tcgcccgccg gatgaccacc gacctccgcc gccgcgagga agaggcgagc cgcctcgcca
     1201 tggtcgtcag ccgcaccgac aacggcgtcg tcatcaccga tgcccagtgc cgcatcctct
     1261 gggtcaatca gtcctacgag cggatcaccg gctatcaact cgccgaaatc cagggcttgc
     1321 gcccgagcga attcctccac ggtcccgaga ccgatccgct gaccgtcgaa atgatgcgcc
     1381 ggcactacca ggagggcaag ccgttcaagg ccgaggtcct caactacgcc cgcaatggcc
     1441 gcaagttctg ggtccatgtc gaagtccagc ccgtctttga tgaagcgcac cacctgaccc
     1501 atttcatggg cctgacctcc gacatcaccg aacggcgcct ctcggaagag gcgctgcgcg
     1561 aaagcgaacg gcgctaccgc ggcctgatcg agtcccagca cgacattatt ctgcggaccg
     1621 acgcgcacgg cctgctgacc tacgtcaacg acgtctattg cgagttcttc gacgtcaccg
     1681 cgctcgacgc cctcggcacc ccctacagcc ccgagatcca tcccgacgac caggccaaga
     1741 cgcgcgaagc cctccagcgc ctctcgatgc ccccct
//
LOCUS       DAOPCO010000451         1762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_53297_length_1762_cov_4.749854, whole genome shotgun sequence.
ACCESSION   DAOPCO010000451 DAOPCO010000000
VERSION     DAOPCO010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53297_length_1762_cov_4.749854"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1719)
                     /locus_tag="PLS90_17530"
     CDS             complement(28..1719)
                     /locus_tag="PLS90_17530"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPK04249.1"
                     /translation="MARKCALGLDFGTNSVRALIVDVANGEELGTAVHGYAHGQQGVI
                     LDGRDPNLARQHPQDYLDGMERAVHEALEQARGQADFTAESIIGIGIDCTGSTPIPLN
                     ADGRPLALTEEFNDDPNAMAWLWKDHTAHAEAAEITELAAKLRPHYLAKCGGGYSSEW
                     YWSKLLHCKRVAPRVFAAAHTWAEHTDWMPAVLTGTEHPSKLQRGICAAGHKAMFNPG
                     WGGYPDAEFLSALDPELARVRATLPDRAANVSQAAGGLTAEWAMRLGLPAGTPVAIGA
                     FDAHLGAVGSGIEPGVLVKIIGTSTCDMMVAPLSGDQRDIPGLCGIVPESILPGHFGF
                     EAGQSAVGDIFNWFVNQIEPGGKAVGSHEELTRLAETLRPGESGLLTLDWHNGNRTIL
                     IDQRLTGLTLGLTLATTPYEIYRSLIEATAFGARVIMERFEEYGCKVERVVNCGGISA
                     KNPLVMQIYADVMGRPLEISRSAQTCALGAAMCGAVVAGQEAGGHADFAAAARAMTGV
                     QPRKFTPIAENQRVYDRLYKLYRQVHDAFGVKGRQESLHNVMKDLLAIRDEMRAK"
BASE COUNT          255 a          701 c          503 g          303 t
ORIGIN      
        1 tgtttgccct atccgccccc gccgcgttca cttcgcccgc atctcatccc ggatcgccag
       61 cagatccttc atcacgttat gcaggctctc ctgccggcct ttcacgccga acgcgtcgtg
      121 aacctggcgg tacagcttgt agagccggtc gtagacccgc tgattctcgg cgatgggcgt
      181 gaacttgcgc ggctgcacgc cggtcatcgc ccgcgccgcc gcggcgaaat cggcgtgccc
      241 gccggcctct tgcccggcca ccaccgcgcc gcacatcgcc gcccccagcg cgcacgtctg
      301 cgcgctgcgc gagatctcca gcggccgacc catcacgtcg gcgtaaatct gcatcaccag
      361 cgggttcttc gccgaaatcc ctccgcagtt gaccacccgc tccaccttgc agccatactc
      421 ctcgaaccgc tccatgatca cccgcgcgcc gaacgccgtc gcctcgatca gcgatcggta
      481 tatctcatac ggcgtcgtgg ccagtgtcag ccccagcgtc agccccgtca gccgctggtc
      541 gatcaaaatc gtccggttgc cgttgtgcca gtccagcgtc agcagcccgc tctcgcccgg
      601 ccgcagcgtt tcggccagcc gcgtcagttc ctcgtgcgaa ccgaccgcct tgccccccgg
      661 ctcaatctga ttgacgaacc agttgaagat gtcgccgacg gccgattgcc ccgcctcgaa
      721 accgaaatga cccggcagga tcgactccgg aacgatcccg cacagacccg ggatgtcgcg
      781 ctggtcgccg ctcagcggcg ccaccatcat gtcgcatgtc gacgtgccga tgatcttgac
      841 cagcaccccc ggctcaatcc ccgaaccgac agcgcccagg tgcgcgtcga acgcgccgat
      901 cgcgaccggc gtccccgccg gcagccccag ccgcatcgcc cactccgccg tcagcccccc
      961 ggccgcctgc gagacgttcg ccgcccggtc cggcagcgtc gcccgcaccc gcgccagctc
     1021 ggggtccagc gcgctcagga attccgcgtc gggatacccg ccccacccgg ggttgaacat
     1081 cgccttgtgc cccgccgcgc agatcccccg ctgcagcttg ctcggatgct cggtccccgt
     1141 cagcaccgcc ggcatccagt cggtgtgctc ggcccacgtg tgggcggccg cgaacacccg
     1201 cggcgcgacc cgcttgcaat gcagcagctt cgaccagtac cactccgacg aatagcctcc
     1261 accgcacttg gccagataat gcgggcgcag tttcgccgcc agctcggtga tctccgccgc
     1321 ttccgcgtgc gccgtgtgat ccttccacaa ccacgccatc gcgttgggat cgtcgttgaa
     1381 ctcctcggtc agcgccagcg gccggccgtc ggcgttcagc gggatcggcg tgctcccggt
     1441 gcagtcgatg ccgatcccga tgatcgattc ggccgtgaag tcggcctggc cccgcgcctg
     1501 ctctagcgcc tcgtgcaccg cccgctccat gccgtcgaga taatcctgcg gatgctggcg
     1561 cgccaggttc ggatcccgtc cgtccaggat caccccctgc tgaccgtgcg catagccgtg
     1621 cacggcggtg cccagttcct ccccgttggc aacgtcgacg atcagtgcac gcaccgagtt
     1681 ggtgccgaaa tccagaccga gtgcgcattt gcgggccatg gtcttctctc ccttgcctgc
     1741 gttcactggc cgggcgcctg cc
//
LOCUS       DAOPCO010000452         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_54490_length_1736_cov_2.963712, whole genome shotgun sequence.
ACCESSION   DAOPCO010000452 DAOPCO010000000
VERSION     DAOPCO010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54490_length_1736_cov_2.963712"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PLS90_17535"
     CDS             complement(<1..832)
                     /locus_tag="PLS90_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04250.1"
                     /translation="MGESTLGKAAAALLHVLTLVLLTATLPAAAPAEASNGDDHPNLW
                     PRYRHDGQMSGRSPLRGGFDAAPGILWSADLGAATVADEAVRLDDVNGDGKTEIIRAL
                     SDRIICQSLDGDILWTADDLPGLQISQIRDWAGDGTRGILVTTSTGTRFHYLMVSGLT
                     GKTTLLETWYNAFGGSLRMGKILADLPGEQFCHWWSGEGFGGVEATGEGSLWSFEKGV
                     DHPTHVFHAQQKGTVYAPSHLFADMDLDGKLDMVMVSHEQLWIYDLGATEPKLYSAWG
                     H"
     gene            complement(858..1472)
                     /locus_tag="PLS90_17540"
     CDS             complement(858..1472)
                     /locus_tag="PLS90_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPK04251.1"
                     /translation="MNCCKRGFTLIELLIVVAIIAILAAIAVPNFLEAQTRSKVSRVK
                     ADQRSLAIGLEAYRVDNNGSLPGFSGNYWYANRYPLIPGLTSPVPYLTSVPEDQFLKQ
                     FFYNTYPPDVARALDWSPAVWDNNIVRALINGGSAPGLVGKPTGVDVGLLDIYVVAAF
                     RDNGHENASFMIRGWGPHWQTQSYVTAYDPTNGTVSAGHIYHFQ"
     gene            complement(1469..>1736)
                     /locus_tag="PLS90_17545"
     CDS             complement(1469..>1736)
                     /locus_tag="PLS90_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPK04252.1"
                     /translation="LDTVSSNAVDSYDYIDVKGTISTTYQQYWGEFTMDGSAAGMVPH
                     ILLSDYPNTSRGQAFILYIDNVDMFIPEFRVEVPNASNHWEVYQ"
BASE COUNT          325 a          491 c          595 g          325 t
ORIGIN      
        1 ggtgacccca ggcggaatag agcttgggtt cggtcgcgcc caggtcgtag atccataatt
       61 gttcgtgcga gaccatcacc atgtcgagct tgccgtccag gtccatgtcg gcaaacaggt
      121 gcgagggggc gtagaccgtt cccttctgct gggcgtggaa cacgtgagtc ggatgatcga
      181 cgcccttctc aaagctccac agactgccct ctcccgtcgc ctccaccccg ccaaaacctt
      241 cgccgctcca ccagtggcag aactgctcgc cggggagatc ggcgaggatc ttgcccatgc
      301 gcaggctgcc gccgaaggcg ttgtaccagg tttcgagcag agtcgtcttg ccggtcaggc
      361 cgctgaccat cagataatga aaccgggtgc cggtgctggt ggtgacgagg atgccgcggg
      421 tgccgtcgcc ggcccagtcg cggatctggg agatctgcag gccgggcaga tcgtcggcgg
      481 tccagaggat gtcgccgtcg agggactgac agatgatgcg gtcggagagg gcgcggatga
      541 tttcggtttt gccgtcgccg ttgacgtcgt cgaggcggac ggcctcgtcg gcgacggtgg
      601 cggcgccgag atccgccgac cagaggatcc cgggcgccgc gtcgaatcct ccccgcaggg
      661 gggagcggcc gctcatttgg ccatcgtgcc ggtagcgcgg ccagaggttg ggatggtcat
      721 cgccgttgga agcttcggca ggcgccgcgg ccgggagggt cgcggtcagg agaacaagcg
      781 tcaggacgtg cagcagcgcc gcggctgcct tgccaagcgt ggattcaccc acaagaatgc
      841 acccttctgt ttacaactta ctggaagtga taaatgtggc ccgcgcttac ggtgccgttg
      901 gtcggatcat aggcggtgac gtaggactgc gtctgccagt gagggcccca gccgcggatc
      961 atgaagctgg cgttctcatg gccgttgtcg cggaaggcgg ccacgacgta gatatcgagc
     1021 aggccgacgt cgacgccggt cggcttgccg accagccccg gggcgctgcc gccgttgatc
     1081 agagcccgga cgatattgtt atcccagacc gcgggggacc aatccagcgc gcgggcaacg
     1141 tcgggtggat acgtattgta gaagaactgc ttgaggaact gatcctcggg cacggacgtc
     1201 aaatacggaa caggcgaagt aagacctgga atcaggggat agcggttggc gtaccagtag
     1261 ttgcccgaga agccgggcaa tgaaccgttg ttgtccacgc ggtaggcctc cagcccgatc
     1321 gcgagtgacc gctggtcggc cttgacccgg gagactttgg agcgggtctg ggcttccagg
     1381 aaattgggaa cggcaatggc cgccagaatc gcaatgatcg ccacgacaat cagcaattcg
     1441 atcagggtga atccacgctt gcagcagttc attggtaaac ctcccaatgg ttcgaggcgt
     1501 ttggcacctc gacgcgaaat tcggggatga acatatcgac gttgtcgata tagagaatga
     1561 aggcttggcc gcggcttgtg ttcgggtagt cgctcagcag gatgtggggt accatgccgg
     1621 ccgcgctgcc gtccatggtg aactcgcccc agtactgctg ataggtggtt gagatcgtgc
     1681 ccttgacgtc gatgtaatca tacgagtcga ccgcgttgga gctgacggtg tcgagc
//
LOCUS       DAOPCO010000453         1735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_54535_length_1735_cov_3.193452, whole genome shotgun sequence.
ACCESSION   DAOPCO010000453 DAOPCO010000000
VERSION     DAOPCO010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54535_length_1735_cov_3.193452"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1735
                     /locus_tag="PLS90_17550"
     CDS             <1..>1735
                     /locus_tag="PLS90_17550"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPK04253.1"
                     /translation="IDIADPQLWWPNGLGPQNIYDATLTLLDPDGTPLDSHTIRIGIR
                     TITTLRNPGSHPDWFDWTYVVNGRPFFARGANYIPPVDLMLRPMPDKARHMVWMARQA
                     GMNMFRIWSGGTLETEDFYRLCDEYGILMQVESPLGMSTLESQVDPEQFRAVVTDLVL
                     AARNHPALAIYCGGNEWNADLPRNRALVEILRQVTTDLDGTRIFHDVSPWGGNSHNYS
                     VGSGGGGGNYYLYNHDASPFVSEAGNWSTPCIETLLSFIPEHEQKAWPPTRESSFGYH
                     TIYNNDWCIGLILGHAAEYGALRNYDDLPAQSMLTKARADSYMAGHVRSAWPRKSGVT
                     LWELKDVWPAITTSNIDYHGIPKTSYYFIKRAYAPMAVISHFDDDTWPAGSTFRAELH
                     AVSDREELTSATVLAEIFDPQLRRLAAREFTINLPADAATTVGTFQWTLPSGEAFPFL
                     YVATLRRPDGTLVASEPYWLNLGALIGYQAPDRERISFLLEDIERPNAVRCDGQEILF
                     EPIIPPVIPRQNALAFLVASAGSGGDALPATFTLDYHSKLERVDVQIPDWQSDGRWLD
                     GVRVPGLRFDHF"
BASE COUNT          313 a          733 c          419 g          270 t
ORIGIN      
        1 atcgacatcg ccgatcccca gctctggtgg cccaacggcc tcggccccca gaacatctat
       61 gacgcgacgc tgaccctgct cgatcccgac ggcacgcccc tcgattccca caccatccgc
      121 atcggcatcc gcacgatcac cacgctccgc aaccccggca gccaccccga ctggttcgac
      181 tggacctatg tcgtcaacgg ccgccccttc ttcgcccgcg gcgctaacta catccccccc
      241 gtcgacctca tgctccgccc gatgcccgac aaggcccgcc acatggtctg gatggcccgc
      301 caggccggca tgaacatgtt ccgcatctgg agcggcggca ccctcgaaac cgaggacttc
      361 taccgcctct gcgacgaata cggcatcctg atgcaggtcg aaagccccct cggcatgtcc
      421 accctcgaat cccaggtcga tcccgaacaa ttccgcgccg tcgtcaccga cctcgtcctc
      481 gccgcgcgca accacccggc cctcgcgatc tactgcggcg gcaacgaatg gaacgccgac
      541 ctcccccgca accgcgcgct ggtcgagatc ctgcgtcagg tcaccactga cctcgacggc
      601 acccgcatct tccacgacgt ctccccctgg ggcggcaaca gccacaacta ctcggtcggc
      661 agcggcggcg gcggcggcaa ttactatctc tacaatcacg acgcctcccc gttcgtcagc
      721 gaggccggca actggtccac cccctgcatc gaaacgctgc tctccttcat ccccgaacac
      781 gaacagaagg cctggccgcc gacccgcgaa agttcgttcg gctaccatac gatctacaac
      841 aacgactggt gcatcggcct catcctcggc cacgccgccg aatacggcgc cctgcgcaac
      901 tacgacgacc tccccgcgca atcgatgctc accaaggccc gcgccgacag ctacatggcc
      961 ggccacgtcc gttccgcctg gcccagaaaa tcaggcgtca ccctctggga actcaaagac
     1021 gtctggcccg ccatcaccac cagcaacatc gactatcacg gcatccccaa aacctcatac
     1081 tacttcatca agcgggccta tgcgccgatg gccgtcatct cccatttcga cgacgacacc
     1141 tggcccgccg gctccacgtt ccgcgccgaa ctccatgccg tcagcgaccg cgaggagctg
     1201 accagcgcca cggtcctcgc cgaaatcttc gacccgcagc tccggcgcct cgccgcccgt
     1261 gaattcacga tcaacctccc cgccgacgcc gccacaaccg tcggcacctt ccagtggacc
     1321 ctcccctcgg gcgaggcctt cccgttcctc tacgtggcga ccctccgccg ccccgacggc
     1381 acgctcgtcg ccagcgaacc ctattggctg aacctcggcg ccctcatcgg ctaccaggcc
     1441 cccgatcgcg agcgcatctc gttcctcctc gaggatatcg aacggcccaa cgccgtccgc
     1501 tgcgacggcc aggaaatcct cttcgagccg atcatccccc ccgtcattcc ccgacagaat
     1561 gccctcgcct tcctggtcgc ctcggccggc tccggcggcg acgccctccc cgccacattc
     1621 accctcgact accacagcaa gctcgaacgc gtcgacgtcc agatccccga ctggcaatcc
     1681 gacggccgct ggctcgacgg cgtccgcgtc cccggcctgc gcttcgatca tttcg
//
LOCUS       DAOPCO010000454         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_58242_length_1661_cov_4.919054, whole genome shotgun sequence.
ACCESSION   DAOPCO010000454 DAOPCO010000000
VERSION     DAOPCO010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58242_length_1661_cov_4.919054"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            542..1048
                     /locus_tag="PLS90_17555"
     CDS             542..1048
                     /locus_tag="PLS90_17555"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPK04254.1"
                     /translation="MHTALRIDTILTPDRVLLNLPQTDKEGVLRAMTDALARQGLIRD
                     SREITRLLLEREALMTTGVKRGFAFPHAFTNQVDESFLTVGVAPAGVEYQALDGHPVE
                     FIFLLLGPPGHQTVHLRILARISRITGDGEMLDLLRAAQSAEEIMDCLTHAERHLNGP
                     APRISSNP"
     gene            1045..>1661
                     /locus_tag="PLS90_17560"
     CDS             1045..>1661
                     /locus_tag="PLS90_17560"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPK04255.1"
                     /translation="MSASPSVRRRVLLVARALPAHVPGGLERHVQDLALGLAGAEVEL
                     HLLTHPLDPAASAPLRAAGLTLHFLPTPHPGRYTLAWLRGVGPLIQALHQRHRFSLIH
                     GQEFALGCWRPRPGDPPLVLSVHGTITSETPLHPDAWRELPWPRRPAALLRYGRRFLF
                     QPAWRRQLRAAARVLIDSAFTRGELIRALPELEPRLALIPLAVRPA"
BASE COUNT          244 a          663 c          462 g          292 t
ORIGIN      
        1 atccgcctgc gagatgaacc cctcgcgcac gagatactcc cccaggctcg gctgactccg
       61 tccctcggcc atggcccctc tgctcccgtg tcccgatact gtggggctca tcacctcctc
      121 ctcccggacg tgccccgtcc ggatcactgg gcggccgtgc cgcaacgggc gtgttcccat
      181 gcgccgccgc gcctcattcc gatgccgtta tgttccataa tccggtcgcg gctgagtggc
      241 gaatacaaaa cctaaattca attatcataa tgacatggca gcgccagctg tcaagcgcaa
      301 acgccgccca tcgtcatcgg catcgctatc gcggtcgccg ccgagctcaa ccgcgcagca
      361 ccccaacgta aaaaatctgt tatgaatttt aaaaatctaa tacgacgccc acccggcctc
      421 cggggcgccg cgccgcccgt tgtcaagaac attgcggcat atttacttgc cgtgtctccg
      481 tgcctgctct agactgcgat tgctggatcg ccctggaaag ggcctcgccg cgactgttcc
      541 catgcatacc gccctgcgca tcgacaccat tttgacgccc gaccgtgttc tgctgaacct
      601 cccgcagacc gacaaggaag gcgtcctgcg cgccatgacc gatgccctgg cccgacaggg
      661 actgatccgc gacagccgcg agatcacccg cctgctgctc gagcgcgaag ccctgatgac
      721 caccggcgtc aagcgcggct tcgccttccc ccatgccttc accaatcagg tcgacgagtc
      781 cttcctgacc gtcggcgtcg cccccgccgg cgtcgaatac caggccctgg acggccaccc
      841 cgtcgagttc atcttccttc tgctcggccc ccccggccat cagaccgtcc atctgcgcat
      901 tctggcccgc atctcccgca tcacgggcga tggggagatg ctcgacctgc tgcgcgccgc
      961 ccagtcggcc gaggaaatca tggactgcct gacccacgcc gagcgtcacc tcaacgggcc
     1021 ggcccctcgc atcagctcca acccttgagc gcgtcgcctt cggtccgccg ccgcgtcttg
     1081 ctcgtcgccc gggcccttcc ggcccatgtg ccgggaggac tcgaacgcca cgtccaggat
     1141 ctggccctgg ggctggccgg cgccgaggtc gaactgcatc tgctgaccca cccgcttgac
     1201 ccggcggcga gtgccccgct gcgcgcggcc ggcctgaccc tgcatttcct tcccaccccg
     1261 caccccggcc gctacacgct ggcgtggctg cgcggcgtcg ggccgctgat ccaggcgctc
     1321 caccagcgcc accgcttctc gctgatccat ggccaggaat tcgccctggg ctgctggcgg
     1381 ccccgtcccg gcgatccccc ccttgtgctc tcggtccacg gcacgatcac gagcgaaacg
     1441 ccgctgcacc ccgacgcctg gcgtgaactg ccctggcccc gccgcccggc ggccctgctg
     1501 cgctacggcc ggcggttcct gtttcaaccg gcctggcgcc ggcagcttcg cgccgccgcg
     1561 cgcgtcctca tcgactcagc cttcacgcgc ggcgaattga tccgcgcgtt gcctgagctc
     1621 gaaccgcgcc tggcgctcat cccgctcgcg gttcggccgg c
//
LOCUS       DAOPCO010000455         1638 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_59475_length_1638_cov_5.739103, whole genome shotgun sequence.
ACCESSION   DAOPCO010000455 DAOPCO010000000
VERSION     DAOPCO010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59475_length_1638_cov_5.739103"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..>1638)
                     /locus_tag="PLS90_17565"
     CDS             complement(148..>1638)
                     /locus_tag="PLS90_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPK04256.1"
                     /translation="RVAVQHEVEALALQGLGAYQVIAANGRPLTQMTAFDPYFVQITR
                     GQPGSRLYRLVLRELDAHQVDPAVEYAKAAKDAYGLPVKVLRMPARDRHESRIIVTLG
                     EFQTLEAARNYHQTLKNEKVRYIYEDRARAEQGEVRLLNRQGSIVARDSRFLRLVPLD
                     IAAGSLRLMELNPSRFKANDLLEARRYRGEMELTINEEGTLTAVNDLWIEYYLYSVVG
                     GEIGQDAPVEALKAQAVVARSEAVAKIQRGIVSSSFFDFFDTPIAQLYRGKQEEDPRV
                     LQAVDATRGEILVWNGQAVDAVYGHSCGGVIASAHDVWGGMHEGYSVRRLDRLVNDKA
                     PDLSNGSDAHAYTSKPTDALCNPEQAGFPAYAKKYFRWTRRFSGAEFSALAGVGRVKD
                     VVVERRSPSGRVTRLKIIGDRTWVADDELEIRRALDHVNSTFFTVLPVQDENGYLKSL
                     TVYGAGFGHGVGMCQMGAFMMGKKGYNYRQILAHYFTDVKIRRLYR"
BASE COUNT          291 a          586 c          474 g          287 t
ORIGIN      
        1 aaatcggccg gcacgccctc ggcgcgcctc tccgccgcca cgcccgaagc cagcacgcca
       61 aggcttagga gaagtaaaca cctgagggaa ttgaacatca ttgcccagaa tcgctatcgg
      121 tatcgcagtc gcaatcgatc ccaaccacta ccgatacaac cgccggatct tcacatcggt
      181 gaaataatgc gccagaatct gccggtaatt atagcctttt ttgcccatca tgaacgcccc
      241 catctggcac atcccgacgc cgtggccgaa tcccgcgcca tacacggtca gactcttcaa
      301 atacccgttt tcatcctgga ccggcagaac cgtaaagaac gtcgaattga cgtgatccag
      361 cgcgcggcgg atctccagct cgtcgtcggc cacccacgtg cggtcgccga tgatcttcag
      421 tcgggtcacg cggccgctgg ggctgcgccg ctcgacgacg acatccttca cccgcccgac
      481 gccggccagc gcgctgaact ccgccccgct gaaccgccgc gtccagcgaa aatatttctt
      541 ggcgtaggcc ggaaaacccg cctgctccgg gttgcacagc gcatcggtcg gcttggacgt
      601 gtaggcatgg gcgtcggatc cgttgctcag atcgggggcc ttgtcgttga ccagccggtc
      661 cagccgccgc acggaatagc cttcatgcat tccgccccag acgtcgtgcg cgctggcgat
      721 caccccgccg cacgaatgcc cataaacggc atcgaccgcc tggccgttcc agacgagaat
      781 ctcgccgcgg gtggcgtcga ccgcctgcag gacgcgcggg tcctcctcct gcttgccgcg
      841 gtagagctgc gcgatcggcg tatcaaaaaa gtcgaagaac gaactggaaa caatcccgcg
      901 ctggatcttg gccaccgcct cgctgcgcgc caccaccgcc tgcgccttga gcgcttcgac
      961 cggagcatcc tgcccgatct ccccgcccac cacgctgtag aggtagtatt caatccacag
     1021 gtcgttgacc gccgtcagcg tcccctcctc gttgatcgtc agctccattt cgccgcgata
     1081 gcgccgcgcc tcgagcaggt cgttggcctt gaaccggctc gggttcagct ccatcagccg
     1141 gagcgatccg gcggcgatgt ccagcggcac caggcgcaga aaccggctgt cgcgcgcgac
     1201 gatcgagccc tgccggttca acaggcgcac ttctccctgc tccgcccgcg cgcggtcctc
     1261 gtaaatgtag cgcaccttct cattcttcag cgtctgatgg tagttgcgcg ccgcctccag
     1321 cgtctggaac tcgcccagcg tcacgatgat ccggctctcg tgacggtcgc gcgccggcat
     1381 ccgcaggacc ttgaccggca agccataggc gtccttggcc gctttggcgt attcgacggc
     1441 ggggtcgacc tgatgcgcgt ccagctcgcg cagcacaagc cgatagagcc ggctgcccgg
     1501 ctgcccgcgc gtgatctgca cgaaataggg gtcgaaggcg gtcatctgcg tcagcggccg
     1561 gccattggcg gcgatgacct gatacgcgcc caggccctgc agcgcgagcg cctcgacctc
     1621 gtgctgaacg gcgacgcg
//
LOCUS       DAOPCO010000456         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_63866_length_1559_cov_4.689495, whole genome shotgun sequence.
ACCESSION   DAOPCO010000456 DAOPCO010000000
VERSION     DAOPCO010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63866_length_1559_cov_4.689495"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(450..1421)
                     /gene="fmt"
                     /locus_tag="PLS90_17570"
     CDS             complement(450..1421)
                     /gene="fmt"
                     /locus_tag="PLS90_17570"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPK04257.1"
                     /translation="MKVVFMGTPEFALPALQRLIASPHRIVLVVTQPDRPKGRGLKPV
                     ASPVHQLAAAHGLEIIQPVSVRREPIDERVRQSGADVTVVAAFGQILPRPLLEATRFG
                     CLNIHPSLLPEFRGAAPVQRCLLEGRDTTGVTIMQLVEELDAGPIIAQQRVEILPDDD
                     ARSVLNNCAVIGADMLLRVLDEAERTGIIDSTPQNDEEATYAPPIRKEEGLIPWRDST
                     EEIMYRLRALTPWPGAYSFLGGRKRLTLLQAEPLWSNEAAQLGVMNKVEPGTVTSLKR
                     GFGFTVKTGSGHLLVTALKPEGKNRMDGQAFVAGRGVSVGDKLGDPA"
BASE COUNT          225 a          443 c          600 g          291 t
ORIGIN      
        1 ccaggccagc gcctcgcggc gcacctgctc ggcgaaggtc accttgcggt ggataatggg
       61 ggtcaggcgg atgttgtgga agttcaggat cgacaccgcg ggggcaattc tccttccgca
      121 gaaatcggcg tttggatctc gtcgtttgcg ctcttgatcg cgatcgattc ggtgggttct
      181 gccgtcccta cgggactctt tgtcctttcc ttcgtctttc ccgccactga agtggcgggc
      241 tgaagtctgg cgtccctgac gggactggct tggggatcag tccggcaggg ccgcggccgt
      301 tcgagtcggt tgtataaggt cccccattat ccgccctgtg ctgaagcacc gacctcctta
      361 cctgcaaatt cgactctcag aagggatata aaggacgtaa gggacacaag gacgcgtgga
      421 gggcatggca tgggatttca ggctgccttt caggcggggt cgccgagttt gtcgccgacg
      481 ctgacgccgc ggccggccac gaaggcctgg ccatccatgc ggttttttcc ctcgggtttg
      541 agcgccgtca ccagcaggtg cccgctgccg gtcttgacgg tgaaaccgaa gccgcgcttg
      601 agcgaggtga cggtgcccgg ctcgaccttg ttcatgacgc cgagctgggc ggcctcgttg
      661 ctccagaggg gttcggcctg gagcagggtg aggcgcttgc ggccgccgag gaaggagtag
      721 gctcccggcc agggggtgag cgcgcgcagg cggtacatga tttcctcggt ggaatcgcgc
      781 caggggatca atccttcctc cttgcggatc ggcggggcgt aggtggcctc ctcgtcgttc
      841 tggggcgtgg agtcgatgat gccggtgcgt tcggcctcgt cgaggacgcg gaggagcatg
      901 tcggcgccga tgacggcgca gttgttgagc accgagcggg cgtcgtcgtc gggaaggatc
      961 tcgacgcgct gctgggcgat gatcgggccg gcgtccagtt cctcgacgag ttgcatgatg
     1021 gtgacgccgg tcgtatcgcg gccctcgaga aggcagcgct ggacgggggc ggcgccgcgg
     1081 aattcgggga gcagcgaggg gtggatgttg aggcagccga agcgggtcgc ttcgaggagc
     1141 gggcgcggga ggatctggcc gaaggccgcg acgaccgtga cgtcggcgcc ggactggcgg
     1201 acgcgctcgt cgatcggctc gcggcgcacg ctgaccggct ggatgatttc caggccgtgg
     1261 gcggccgcca gttgatggac gggcgaggcg accggtttca ggccgcggcc tttggggcgg
     1321 tcgggctgcg ttacgaccag gacgatccgg tgcgggctgg cgatgaggcg ttggagggcc
     1381 ggcagcgcga actccggcgt tcccatgaag acgactttca taatcggtcc ttgcccgacg
     1441 gggctggctg cggcgcgccc ccggtgcgcg ggtcggtgcg gggggcgcga agatgctcca
     1501 tgatagcgaa tccgggcatg gcattgccag caggcaggat cgggacggtt ggggcggcg
//
LOCUS       DAOPCO010000457         1366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_77923_length_1366_cov_4.843631, whole genome shotgun sequence.
ACCESSION   DAOPCO010000457 DAOPCO010000000
VERSION     DAOPCO010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77923_length_1366_cov_4.843631"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PLS90_17575"
     CDS             complement(<1..1127)
                     /locus_tag="PLS90_17575"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPK04258.1"
                     /translation="MQRVASIVLAGGRGPDFGPLSERRTKAAFPIAGTYRIIDFVLSN
                     LCLSGIRQVGIITQYLPAPLMSHIGSGRSWDFDMADRSLKFMTPFLGYSETRWFNGSA
                     DAIAKNINLFDLNEIDHLLILSGEHVYQMDYRELLEFHVRQQADVTLACVDLPPSRQN
                     PRFGHVVVNGEGRVVSFVEKPERSVGSLVSMGVFCFRRDVLLHLLEAGRPDEGEEFTL
                     AGDVLQPFVKHLRAVAWTFRRPWHYLGNLQEYHEFHMRLARGEIELFERDWEVMTNFS
                     DRTLSSRSPAFFAAGSRARDSIISPGSVIEGVVESSVLSPGVFVGRGAVVRDSIILHD
                     VRIEEGARLERVIADKDATFAGGARVGVPDVGAGGHPLTIIP"
BASE COUNT          252 a          512 c          390 g          212 t
ORIGIN      
        1 ggaatgatcg tcaacggatg ccccccggcg ccgacgtcgg gcacgcccac gcgcgcgccg
       61 cccgcgaacg tcgcatcctt gtccgcaatc acccgctcaa gccgcgcgcc ttcctcgatc
      121 cgcacgtcgt gcaggatgat cgaatcgcgg acgacggcgc cgcgcccgac gaacaccccc
      181 ggcgacagca cgctgctctc gacaaccccc tcgatcaccg agccgggcga gatgatgctg
      241 tcgcgcgccc ggctcccggc ggcgaagaac gccggtgacc gcgacgacag cgtccggtcc
      301 gaaaaattcg tcatcacttc ccagtcgcgc tcaaacagct cgatctcgcc ccgcgccagt
      361 cgcatgtgga actcgtgata ctcctgcaga ttgcccaggt agtgccacgg ccggcggaac
      421 gtccaggcga cggcgcgcag atgcttgacg aacggctgca gcacgtcgcc cgccagcgtg
      481 aactcctccc cctcgtcggg acggcccgct tccagcagat gcagcagcac atcgcgccgg
      541 aaacagaaca cccccatcga gacgagcgat cccacgctgc gctcgggctt ctcgacaaac
      601 gaaaccaccc gcccttcgcc gttcaccacc acatgcccga agcgcggatt ctgccgcgac
      661 ggcggcaggt cgacgcaggc cagcgtcacg tcggcctgct gccggacgtg gaactccagc
      721 agttcgcggt aatccatctg gtagacatgc tcgccggaga ggatcagcag atggtcgatc
      781 tcattcaggt cgaacaggtt gatgttcttg gcgatggcgt cggccgagcc gttgaaccag
      841 cgcgtttcgc tgtagcccag aaacggggtc atgaacttga ggctgcggtc ggccatgtcg
      901 aaatcccacg agcggcccga gccgatgtgc gacatcagcg gcgccggcag atactgcgtg
      961 atgatcccca cctggcggat gccggaaagg catagattac tgagaacgaa gtcgatgatc
     1021 cggtaggtcc cggcgatcgg gaacgccgct ttggtccggc gctccgacag cggcccgaaa
     1081 tccggcccgc gcccgccggc gagaacgatc gatgccaccc gttgcatgtc ggtcttgatt
     1141 ccgtccgccg agagctgatt ccgatcccgc ccgctcaaaa ccttatcacg acccctcagc
     1201 cgggccaagg cttgatatcc gtcccccccg tcgtcccccg tcctcgtcat cgccccccac
     1261 ggcctcccca gaattcggct cccggctctc ggccctcgtc ctcaagcgca tccgctcatc
     1321 acccgatgaa aaaaaatgcg ggtctccccc cgcccccgtt cccccc
//
LOCUS       DAOPCO010000458         1288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Sumerlaeota bacterium isolate AMR_MDS_3457
            NODE_85312_length_1288_cov_6.030819, whole genome shotgun sequence.
ACCESSION   DAOPCO010000458 DAOPCO010000000
VERSION     DAOPCO010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865192
            Sequence Read Archive: SRR5007224
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Sumerlaeota bacterium (wastewater metagenome)
  ORGANISM  Candidatus Sumerlaeota bacterium
            Bacteria; Candidatus Sumerlaeota.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 21:15:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,516
            CDSs (total)                      :: 3,473
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 1 of 27
            Pseudo Genes (incomplete)         :: 24 of 27
            Pseudo Genes (internal stop)      :: 2 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Candidatus Sumerlaeota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85312_length_1288_cov_6.030819"
                     /isolate="AMR_MDS_3457"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2107588"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLS90_17580"
     CDS             complement(<1..1173)
                     /locus_tag="PLS90_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPK04259.1"
                     /translation="MAVSHPATSPHPQSHPIGEHLARRILILDGAMGTMIQGFGLAEA
                     DFRNDPRLTDLPRDLKGNNELLCLTRPDVIEKIHADFLDAGADIIETNTFNANAVSQA
                     DYGTVHLVREINLAAARLARAAADRQSAATPDRPRFVAGAIGPTNRTASMSPDVNDPG
                     YRAITFDDLVEAYAEQARALIEGGVDLLLCETVFDTLNLKAALYAIDRVQGELGARLP
                     VMISVTITDRSGRTLSGQTVEAFWYSIEHARPLSVGINCALGADDMRPYVEELAGLAD
                     CLVSVYPNAGLPNAFGGYDDTPEKMAEVLGDFAQQGWLNMVGGCCGTTPAHIAAIAAA
                     VAPHPPRVPVGRPRTSRFSGLEPLRIEPETNLIMVGERTNITGSPRFARAIREGDFD"
BASE COUNT          208 a          365 c          505 g          210 t
ORIGIN      
        1 gtcgaagtcg ccctcgcgga tggcgcgggc gaagcgcggc gagccggtga tattggtccg
       61 ttcgccgacc atgatcagat tggtctccgg ctcgatgcgc agcggttcca ggccgctgaa
      121 ccggctggtc cgtgggcggc cgacggggac gcgcggggga tgcggcgcga cggccgcggc
      181 gatggcggcg atgtgggccg gcgtggtgcc gcagcagccg cccaccatgt tcagccaacc
      241 ctgctgtgcg aaatcaccca gcacttcggc cattttttca ggggtgtcgt cgtagccgcc
      301 gaaggcgttg ggcaggccgg cgttggggta gacgctgacc aggcagtcgg ccaggccggc
      361 cagttcctcc acatagggac gcatgtcgtc ggcgccgagg gcgcaattga tgccgaccga
      421 gagcgggcgc gcgtgttcaa ttgaatacca gaaggcttcc acggtctggc ccgagagcgt
      481 gcggcccgag cggtcggtga tggtgaccga gatcatcacg ggaaggcggg cgccgagctc
      541 cccctgcacg cggtcgatgg catagagcgc ggccttgaga ttgagcgtgt cgaagaccgt
      601 ttcgcagagg agcaggtcga cgcccccctc gatcagtgcg cgggcttgtt cggcgtaggc
      661 ttcgacaagg tcgtcgaacg tgatggcgcg atagccggga tcgttgacgt cgggcgacat
      721 cgaggcggtg cggttggtgg ggccgatggc gccggcgacg aagcgcgggc ggtcgggcgt
      781 ggcggcggat tggcggtcgg ccgcggcgcg ggccaggcgc gccgcggcca ggttgatctc
      841 gcgcacgagg tggaccgttc cataatcggc ctgcgagacg gcgttggcgt tgaacgtgtt
      901 ggtttcgatg atgtcggcgc cggcgtcgag gaaatcggcg tgaatcttct cgatgacgtc
      961 cggccgcgtc agacagagca gttcgttgtt gcccttgagg tcgcggggga ggtcggtcag
     1021 ccgcgggtcg ttgcggaaat cggcttcggc gaggccgaaa ccctgaatca tcgtccccat
     1081 tgcgccgtcg aggatcagga tgcggcgcgc aaggtgctcg ccaatcggat gggattgagg
     1141 atgcggggag gtcgccgggt gagaaaccgc catgaaaagg ccgccttgga ggtaatccag
     1201 ccgcgcggga cgaaccgggc cgggccgtgt gcactcagag tcagtttaac aggggaacgc
     1261 gccgacgaca aggaagggac gatgcggg
//
